LOCUS       DASVPL010000001         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_312580, whole genome shotgun sequence.
ACCESSION   DASVPL010000001 DASVPL010000000
VERSION     DASVPL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312580"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1231
                     /locus_tag="VF517_00005"
     CDS             293..1231
                     /locus_tag="VF517_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX8051345.1"
                     /translation="MDAIVVGIDVSKDRLDVAVRPSGEVFAVARDADGIDGLIARIAP
                     LCPTAVAVEATGGYETVVAASLAAAGLAVVVVNPAQVRGFARALGQRAKTDPIDARII
                     AHFVEATKPEIRPLADAETRMLADLVARRRQIVQMIVAERQRQRRLPDKRLQKSIARL
                     LAALQKELSSLETDIDDAVRGSPAWRDKEDLLASVPGIGKIIARTLLAELPELGALDR
                     RKIAALVGLAPWTRQSGQWKGKSFIGGGRADVRAALFTGAMVAARYNPDLKVFRDKLV
                     QAGKPKLVALVATARKLLTILNAIVRDQKPWRQQTA"
     gene            complement(1393..>2669)
                     /locus_tag="VF517_00010"
     CDS             complement(1393..>2669)
                     /locus_tag="VF517_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH family hydrolase"
                     /protein_id="HEX8051346.1"
                     /translation="DDSGRYPTIERFRPAPGYGQRPARQRPAPAYGGGYGAQQYYPGQ
                     LYYPGMQVYPEARQPAYGGYERSERGYERRAYGGEARPRVYRRAEPKRRQVPAAPRAA
                     QVKPKVEPSTFLVVFGDNLAEQVSSGLEDAYDEKPEIEVVDESKADTGLVRADVHDWP
                     KQIQDYLNGDPKITAAIVMLGANDRQAIREGETSHDPLSDRWRELYRERVDAALRPFV
                     DKRIPFVWVGAPPVKNEKAMADLMAINDILRERVARAGGTYVDIWPGFVNAENRYAPS
                     GPDVEGQTARLRLNDGVQFTTAGARKAAHFVVTELKRIIDEKATGAAVAALPKTPEIP
                     APAVPEQATPAVAAAPEAAAPAPVVKPPRPIAGPVLPLTKPETSPGGTLINARPKLEG
                     EAGPIAERALRDGVPPEPKPGRADDFTWPPKS"
BASE COUNT          368 a          932 c          975 g          394 t
ORIGIN      
        1 gagttcgctc gtgttcatcc gcggcgacat cgtctgcggc cgccgcacgg tcgccacggc
       61 gacggggatc tggaagatcc tgtgaaacgg ctctgaacgg ccctgcggct gcccttgtaa
      121 ctatgatggt gccgactcag gcaagatgct tggcgcggtg gcgggcgcta gaccgcaagc
      181 cctgagggac gccaagcccg tgggtgagca agggtcatcg cccgtcgttc catcgaaccc
      241 gaacagtcgc ttgggccgcc agcgaagccc gcttgcgcaa ggacgggaca ggatggacgc
      301 catcgttgtc ggtatcgacg tgtcgaagga ccgcttggac gtggccgtgc ggccgagcgg
      361 ggaggtgttc gcggtcgctc gcgacgcgga cggcatcgac gggctcatcg cccggatcgc
      421 gccgctgtgc ccgacggccg tggcggtgga ggcgaccggg ggctacgaga cggtcgtcgc
      481 ggcgagcctg gcggcggcgg ggctcgccgt cgtggtggtg aaccccgccc aggtgcgcgg
      541 cttcgcgcgg gcgctcggcc agcgggccaa gaccgacccg atcgacgcgc gcatcatcgc
      601 ccacttcgtc gaggcgacga agcccgagat ccggcccttg gccgacgctg agacgcggat
      661 gctcgccgac ctcgtcgcca ggcgccggca gatcgtccag atgatcgtgg ccgagcgtca
      721 gaggcagagg cgtctgcccg acaagcgcct gcagaagagc atcgcccggc tgctcgccgc
      781 gctgcagaag gagctctcga gcctcgagac cgacatcgac gacgcggtgc gcggctcgcc
      841 ggcctggcgc gacaaggagg acctgctcgc ctccgtgccc ggcatcggca agatcatcgc
      901 gcgcaccctg cttgccgaac tgcccgagct cggcgccctc gaccgccgca agatcgccgc
      961 cctcgtcggt ctcgcgccct ggacgcgcca gtccggccaa tggaaaggca agagcttcat
     1021 cggcggcggc cgggccgacg tccgcgccgc cctcttcacc ggcgccatgg tcgcggcgag
     1081 gtacaacccc gacctcaagg tcttccgcga caagctcgtc caggccggaa agccgaagct
     1141 cgtcgccctc gtcgccaccg cccgcaagct cctcaccatc ctcaatgcca tcgttcggga
     1201 ccaaaagccg tggcgccaac aaaccgcttg accgctaaga cagtcgctca cccggtcgcg
     1261 gctgacgccg cgactcgacc tctcccgcac atgcgggaga ggggacgcgc gcctcgcctc
     1321 gcccgcgcag cgggagaggt cgagtggcgc gtcagcgccg gccgggtgag ggcatcggcg
     1381 cgcttcgagc gatcaggatt tcggcggcca ggtgaagtcg tcggcgcggc cgggcttggg
     1441 ctcgggcgga acgccgtcgc gcagcgcccg ctcggcgatg gggccggcct cgccctcaag
     1501 cttcggacga gcgttgatga gggtgccccc cggcgaggtc tcaggcttcg tcaggggcag
     1561 caccggcccg gcgatgggac ggggcggctt gaccacgggc gccggcgcgg cggcctcggg
     1621 ggcggccgct acggccgggg tggcctgctc cggaacggcc ggcgccggga tctcgggtgt
     1681 cttgggcagg gccgcgacgg cggctccggt cgccttctcg tcgatgatgc gcttcagctc
     1741 cgtgacgacg aaatgcgccg ccttgcgggc gccggcggtc gtgaactgca cgccgtcgtt
     1801 caggcgcagg cgcgccgtct ggccctcgac gtccggcccg ctcggcgcat agcggttctc
     1861 ggcgttgacg aagcccggcc agatgtcgac gtaggtgccg cccgcccggg cgacacgctc
     1921 gcgcaggatg tcgttgatgg ccatcaggtc ggccatggcc ttctcgttct tcaccggcgg
     1981 cgcgccgacc cacacgaacg gaatgcgctt gtccacgaag ggtcgcaggg cggcgtcgac
     2041 gcgctcgcgg tagagctcgc gccagcggtc cgacagggga tcgtgcgagg tctcgccctc
     2101 gcggatggcc tggcggtcgt tggcgccgag catcacgatg gccgccgtga tcttggggtc
     2161 gccgttgagg taatcctgga tctgcttcgg ccagtcgtgc acgtcggcgc gcacgagacc
     2221 cgtatcggcc ttgctctcgt cgacgacctc gatctcgggc ttctcgtcgt aggcgtcctc
     2281 gaggccgctc gagacctgct cggcgaggtt gtcgccgaac acgacgagga acgtcgaggg
     2341 ctcgaccttc ggcttgacct gggctgcgcg gggcgcggcc ggcacctggc gccgcttcgg
     2401 ttcggcgcgg cgatagacgc ggggccgggc ctcgcccccg taggcgcgac gctcataacc
     2461 gcgttcggag cgctcgtaac cgccgtacgc cggctggcgc gcttccggat agacctgcat
     2521 gcccgggtag tagagctggc ccgggtagta ctgctgcgcg ccgtagccgc cgccgtaagc
     2581 gggcgcgggc cgttgccggg cagggcgctg gccgtaacca ggagccggac ggaagcgctc
     2641 gatcgtcggg taccgcccgc tgtcgtcgt
//
LOCUS       DASVPL010000002         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_39292, whole genome shotgun sequence.
ACCESSION   DASVPL010000002 DASVPL010000000
VERSION     DASVPL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39292"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1147
                     /locus_tag="VF517_00015"
     CDS             233..1147
                     /locus_tag="VF517_00015"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8051347.1"
                     /translation="MELVTGATGYVGGRLVERLTREGRSVRAMARNPARLEPLDGVEA
                     VRADVLRDEGLEEALDGVSSAYYLLHSMEAGDEDFAARDRRAATNFARAARAAGVEKV
                     VYLGGIAPPPGTRHSPHLTSRLEVESILLRELPRSVGLRASILIGARSSSLRMMVRLV
                     ERLHFLPLPAWAGNRTAPIDERDAIEFLARAPQVEEAEGRSLDIAGPDVMTFGGMVER
                     IAELMGVGRSPIRLSVSQTPAASAVVAELTGQPVDLVRSLMESLETDVLPRDRDAERI
                     LGLRPRSFDRAVEHALGEWERTEALAAR"
     gene            1144..1602
                     /locus_tag="VF517_00020"
     CDS             1144..1602
                     /locus_tag="VF517_00020"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8051348.1"
                     /translation="MTKLERAIEIDAPRERVYEVLTDPRCLGEWVTIQEELEEAPEGD
                     LVEGDTLRQRMKVAGQRFKLAWTVVEVDPPTRIVWEGCGPVGSKARAVYELEESARGG
                     TRFSYLNEYAMPGGPLGRLAGRAVTKASGGEADRSLERLKALVERRVAAG"
     gene            1744..2685
                     /locus_tag="VF517_00025"
     CDS             1744..2685
                     /locus_tag="VF517_00025"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX8051349.1"
                     /translation="MIVRLDRWAAQLPPPSDPDPDGAAAVQELMGGKFGEMSTLMNYT
                     FQSFNFRGKQGARPFYDLVANIAAEEFGHIELVAATINTMLTGASLDGAKGSRNAQQF
                     IAGGKSALPQDSNGRAWTGDYVTATGDLVEDLTHNFFLETGARQNKLKVYEMTDHPAA
                     RALTGYLLCRGGVHQVAYARAIEKLTGADLTKLFPSPRIPTEKIPECQPHIEKGEHRK
                     LYRFSPGDYGEIVAVFNGPHPETGEELEVVDGPPAGVLPQDLPPEPETFAPDYAPEEI
                     AEIARRLRKAAGLGDEPRAEVANSPSGLVDTIKEKVS"
     gene            2752..3423
                     /locus_tag="VF517_00030"
     CDS             2752..3423
                     /locus_tag="VF517_00030"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX8051350.1"
                     /translation="MAKVVDLTQPLGPQTVMWPGNPPLVAEQVGGYATDGFYARAIAL
                     AEHSGTHFDAPAHFAPDGMRAHAVPPERLVVPCAVVDVSAECSADPDYTLDTDRLARD
                     EAVHGPLPEGGALLLRTGWDAHAGDAERYVESMSFPGYGVDAAELAIERGCVGLGTDT
                     LSIDAGHDPAYPVHHLTLPAGLWHLEGLVNLAALPPRGATLFVGALKLVDGSGTPARV
                     LALVG"
     gene            3420..>3767
                     /locus_tag="VF517_00035"
     CDS             3420..>3767
                     /locus_tag="VF517_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8051351.1"
                     /translation="MTIPELSTERLRLRPFSQEDTDAFAALCADDEVMRWLGGTMDRS
                     TAWRHMATLVGHWDLHGFGRWAVEVRESGALVGHVGLWFPEGWPELEVGWTLAPAFRG
                     RGYATEAASAAVAW"
BASE COUNT          518 a         1345 c         1409 g          495 t
ORIGIN      
        1 gtcacaggac acgctctttc ccttcgccac cgtggcgaac ctccccagtt gctccgagtt
       61 gccgggcccg atccgcggcc cgaccgatcg cggcccatcc aacaacaatt cgcccccctc
      121 cgccaccccg cgtttccgca aatgcctgct tttcgttttg ggcctcccac ggaccccccg
      181 gtatttgcgg gcaatccgaa atcggcaccc ggttcgtacg ctccggaacg cgatggaact
      241 cgtgaccgga gcgaccggat atgtgggcgg acggctcgtc gaacggctga cgcgcgaggg
      301 ccgctcggtg cgtgcgatgg cgcgcaaccc cgcgcggctc gagccgctgg atggcgtcga
      361 ggccgtgcgc gccgacgtgc tgcgcgacga gggcctcgag gaggcgctcg acggcgtcag
      421 ctcggcgtac tacctcctcc actcgatgga ggccggcgac gaggacttcg ccgcgcgcga
      481 ccgccgcgcc gccacgaact tcgcccgcgc cgcccgcgcc gcgggggtcg agaaggtcgt
      541 ctacctcggg gggatcgcgc cgccgcccgg gacgcggcac tcgccgcacc tcacctcgcg
      601 gctcgaggtc gagtcgatcc tgctgcgcga gctgccacgc tcggtggggc tgcgggcgtc
      661 gatcctgatc ggggcgcgat cgtcctcgct gcgaatgatg gtccggctgg tcgagcggct
      721 gcacttcctg ccgctccccg cctgggcggg caaccgcacg gccccgatcg acgagcgcga
      781 cgccatcgag ttcctcgcgc gtgcgccgca ggtcgaggag gcggagggcc gatcgctcga
      841 catcgccggg ccggacgtga tgaccttcgg tgggatggtg gagcggatcg cggagctcat
      901 gggcgtcggg cgctcgccga tcaggctgag cgtgagccag acgccggcgg ccagcgccgt
      961 cgtggccgag ctgaccgggc agccggtcga cctcgtgcgc tcgctcatgg agtcgctcga
     1021 gaccgacgtg ctcccgcgcg accgcgacgc cgagcggatc ctcggcctgc ggccgcggtc
     1081 gttcgaccgc gctgtcgagc atgcgctcgg cgagtgggag cggacggagg cgctggcggc
     1141 gcggtgacga agctggagcg cgcgatcgag atcgatgccc cgcgcgagcg cgtctacgag
     1201 gtgctcaccg acccgcgctg cctgggcgag tgggtcacga tccaggagga gctcgaggag
     1261 gcccccgagg gcgacctggt cgagggcgac acgctgcgcc agcggatgaa ggtcgcgggc
     1321 cagcggttca agctggcctg gaccgtcgtc gaggtcgacc ctcctacgcg gatcgtgtgg
     1381 gaggggtgcg ggccggtcgg gtcgaaggcg cgcgcggtct acgagctcga ggagagcgcc
     1441 cgcggcggca cgcgcttctc gtacctgaac gagtacgcca tgccgggcgg ccccctgggc
     1501 cgcctcgccg gccgcgcggt cacgaaggca tcgggcggcg aggccgaccg ctcgctcgag
     1561 cggctcaagg cgctggtcga gcgccgggtc gccgcggggt agccgagcgc cgggtcgcgg
     1621 cggggtagcc gagcgccgcg gcgctcgccc gcgcggcttc gcgcctgccc cggaggcgct
     1681 tgttagggcg ccctaagtcg gggtagggcc ggggagtccg ctctcgaccg aagggttgtc
     1741 tcgatgatcg tccgtctcga ccgctgggcc gcgcagctcc cgccgccgtc cgatcccgat
     1801 cccgacggag ccgccgccgt gcaggagctg atgggcggca agttcggcga gatgtcgacg
     1861 ctgatgaact acacgttcca gtcgttcaac ttccgcggca agcagggcgc gcggccgttc
     1921 tacgacctcg tggcgaacat cgcggccgag gagttcggcc acatcgagct ggtcgcggcg
     1981 acgatcaaca cgatgctgac cggcgcgtcg ctcgacggcg cgaagggctc ccgcaacgca
     2041 cagcagttca tcgccggggg caagtccgcc ctcccgcagg actcgaacgg acgcgcctgg
     2101 accggcgact acgtgaccgc gacgggcgac ctcgtcgagg acctgaccca caacttcttc
     2161 ctcgagacgg gcgcgaggca gaacaagctg aaggtctacg agatgaccga ccacccggcg
     2221 gcgcgcgcgc tgaccggcta cctgctgtgc cgcggcggcg tccaccaggt cgcctacgcg
     2281 cgcgcgatcg agaagctcac cggcgcggac ctgacgaagc tgttcccctc gccgcggatc
     2341 ccgaccgaga agatccccga gtgccagccg cacatcgaga agggcgagca ccgcaagctc
     2401 taccgcttct cccccggcga ctacggcgag atcgtcgccg tgttcaacgg cccgcatccc
     2461 gagacgggtg aggagctcga ggtcgtcgac ggcccgcccg ccggcgtgct gccgcaggac
     2521 ctcccgccgg agccggagac gttcgcgccg gactacgccc cggaggagat cgccgagatc
     2581 gcgcgccggc tgcgcaaggc cgccgggcta ggcgacgagc cgcgagccga ggtggcgaac
     2641 agcccgagcg ggctcgtcga cacgatcaag gagaaggtga gctaggacgc acgagcgccc
     2701 cgcgggcctt gggccggcgg ggcgctcgga cgatccccat actccgcgcg gatggcaaag
     2761 gtggtggacc tcacgcagcc gctcgggccg caaaccgtga tgtggccggg caacccgccg
     2821 ctggtcgcgg agcaggtcgg cggctacgca acggacggct tctacgcgcg cgcgatcgcg
     2881 ctcgccgagc actcgggcac gcacttcgac gccccggcgc acttcgcgcc ggacggcatg
     2941 cgggcgcacg cggtcccgcc cgagcgcctg gtggtgccat gcgccgtggt ggacgtgagc
     3001 gccgagtgct ccgccgaccc cgactacacg ctcgacaccg accggctggc gcgcgacgag
     3061 gccgtgcacg gcccgctgcc cgagggcgga gccctgctgc tgcggaccgg ctgggacgct
     3121 cacgccggcg acgccgagcg ctacgtcgag tccatgagct tccccggcta cggagtcgat
     3181 gccgccgagc tggcgatcga gcgcgggtgc gtgggcctcg ggaccgacac gctgagcatc
     3241 gatgccgggc acgaccccgc gtacccggtg caccacctca cgctgcccgc cgggctgtgg
     3301 cacctggagg ggctcgtgaa cctcgccgcg ctgccgccgc gcggcgcgac gctgttcgtc
     3361 ggcgcgctca agctggtgga cgggtcgggc accccggcgc gcgtgctagc gctcgtcgga
     3421 tgacgatccc cgagctcagc acggagcggc tgcggctgcg cccgttcagc caggaggaca
     3481 cggacgcgtt cgccgcgctg tgcgcggacg acgaggtgat gcgctggctc ggcggcacga
     3541 tggaccgatc gacggcgtgg cggcacatgg cgacgctcgt gggccactgg gacctgcacg
     3601 gcttcggccg ctgggcggtg gaggtgcgcg agagcggcgc gctcgtcggt cacgtcgggc
     3661 tgtggttccc ggagggctgg ccggagctcg aggtggggtg gaccctggcg cccgcgttcc
     3721 gcgggcgcgg ctacgcgacc gaggcggcgt ccgctgcggt ggcgtgg
//
LOCUS       DASVPL010000003        24198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_117614, whole genome shotgun sequence.
ACCESSION   DASVPL010000003 DASVPL010000000
VERSION     DASVPL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24198
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117614"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF517_00040"
     CDS             complement(<1..189)
                     /locus_tag="VF517_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051352.1"
                     /translation="MGRGIGALGATLLTLGVLAASAAASTVPADVLGPNGDQHIDYPP
                     WDEAYGAPGFAVGAVGDVN"
     gene            327..399
                     /locus_tag="VF517_00045"
     tRNA            327..399
                     /locus_tag="VF517_00045"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:360..362,aa:His,seq:gtg)
     gene            complement(700..3105)
                     /locus_tag="VF517_00050"
     CDS             complement(700..3105)
                     /locus_tag="VF517_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX8051353.1"
                     /translation="MVALNWNRSLRLSAAFVLASLALALGAAQASAETLVSLGDSFSS
                     GEGAGSYDEGTDDDVGFPRKHRCHRSGNAWPRLLGVSRDHHLACSGAIIKSLVEGQAE
                     KPPDDIGQIERLRNLAAETRFDRVLLTMGGNDIGFASRIRDCFVHTRLCLNNLSAIKR
                     DLKKRVKPQLIQRYKEVRLAANASLLVVGYPNIVPSADKKIEGCWADKRDRKRAAELI
                     KTLDATFASAASTAGVDFVSLADVLKGHELCTRDSASWVRKINLKDGQEQAHPNQLGQ
                     LAMAKRVLEWINAHQAPCTPSDNVAAIVDDSGSMAGSDPEGIRRRALELLVSKPAAQM
                     RTFGAVEFAGDAGSLFEPGLVADSRPRMLEALAALADDGIGENDSGGTDYNDAFRATA
                     EHQPGATSRIFLTDGAHNDGEYEELHAGGPPTHVIGLDIGPGGEGNENAERLLQIADE
                     TGGRYFPLKLQDDDSAATQVARLQGVVNEIDAQLTCAKSDATTPVRLAEPGLPSSTVR
                     GLLASGAPAVEVVVSWGTDDVDVDLASADVRNRNGRVIADLDGGPGQSRLQPNVVEGR
                     TFDVVTLPVPRDGRSLGLSVSAAELPTPTDVTIQIRPTSTVAPPGRSDVGGPQSGGPP
                     ASEQPQPQPQPQPQPRPPPPPVRVSAYDHYKPAAAGHAMCRGNPGNSASMPGGTATQT
                     FTVPSGVASIDSALVQIDPDGSVTGHLAVYVNGTLRAQAASAAGGDTQFSFSAVPVRG
                     GDQVSLSISFTATFGKIITVYTTGSQPTGGGNFTASNSCPAGAPNVSTPNGLRAAVYG
                     WSG"
     gene            3959..4876
                     /locus_tag="VF517_00055"
     CDS             3959..4876
                     /locus_tag="VF517_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051354.1"
                     /translation="MFAAALVAVGGCGADDTKPVNLDDSSREPRNLDKRIEDAERRLR
                     RLQEGRSEPRAPRTTVGLRGAEDFLASLNAKAGVTAGPPGADEPETAGDLTSGPAWST
                     IKVPIALRVLDRAGGVSALGDTKRSQIESAITRSDNGAAARLFASLGSTAEAAADVQD
                     VLRQAGDDETHVSTEGRGEFSPYGQTDWALARQHRFMAALAGGCLGDKNNADYLLSLM
                     GAVTSDTWGLGSAGGPAKWKGGWGPGPEGRYLVRQMGVIEFGSRKLVVTLAAIPHDGQ
                     FATGQRVATAIAEWLRRHARGLAGPVNRC"
     gene            5614..6039
                     /locus_tag="VF517_00060"
     CDS             5614..6039
                     /locus_tag="VF517_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051355.1"
                     /translation="MLTPISQRPRFPYYWRVTKVRFRDLTGDGLPEIVWELFTSGGTG
                     SSPSLKGVDRWNGQSAARVFRFHNVDRKPPPDFEYVIGVSWRIVGAVDGGLAEIETSE
                     SLHRSDDGTCCPSAYRVTRHRWDGTTIAPVSGSETIEDQ"
     gene            complement(6199..6573)
                     /locus_tag="VF517_00065"
     CDS             complement(6199..6573)
                     /locus_tag="VF517_00065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX8051356.1"
                     /translation="MSRFTDGELDYLRSGRRLGRIATVGADGTPHVVPVGWSYNPEHD
                     TIDVTGRDLAATKKFRDAQRSGRAAIVIDDLASTNPWRPRAVEVRGRAEAIREPRPTI
                     RVHPERIVSWGLEGGRQSRSVS"
     gene            complement(6575..6913)
                     /locus_tag="VF517_00070"
     CDS             complement(6575..6913)
                     /locus_tag="VF517_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051357.1"
                     /translation="MSTRKEGTVAVAILSEVPEGTVEMYEAVTERLGGKGALAPGHLV
                     HVVGERGEGGLRVFDVWETYEDFERFDEEHLHPAVREVMGDRVSPPLRTRYEVYDLAI
                     ADQSTPVGAG"
     gene            complement(6910..7488)
                     /locus_tag="VF517_00075"
     CDS             complement(6910..7488)
                     /locus_tag="VF517_00075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX8051358.1"
                     /translation="MRIIGLCGSLRGGSYNRKLLDTVGSALPNGAELELYEGIGELPP
                     YSEEIDVEPAPPAVRRLREAIEHADALLIATPEYNSSIPGALKNAIDWASRPFPDNSL
                     RDKPAAVVGASRSPFGAAWAQAELRKVLKTCGADVIDREVPVGRVHEQFDAAGDLRDA
                     LLRGQLAGVAGELVRRATGSDGGPGDELSRAA"
     gene            complement(7687..8184)
                     /locus_tag="VF517_00080"
     CDS             complement(7687..8184)
                     /locus_tag="VF517_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8051359.1"
                     /translation="MNERARSPEDVSLTRGLVHVMLAMNATYTRVSRELGLTAQQAEL
                     LCLAQRRASVGELAHIMSCDRSNVSRLLDRVAGRGLVARRDAEGDGRMRVVELSDEGQ
                     ELVDRFTERLGAETQRLFGTWSEPEQRELALKLNEIANTLDASNAPAPQRRPAPSLDS
                     ALEAF"
     gene            8297..8974
                     /locus_tag="VF517_00085"
     CDS             8297..8974
                     /locus_tag="VF517_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8051360.1"
                     /translation="MITPGGADRLDVERRGSVTVVRMTHDENRFHPELLAGIEAVLDE
                     VEFTEDAGALVVTGSGKFFSNGLDLEYMSSHPDEVDEVLARVHALFGRVLGLEVPTVA
                     AINGHAFAAGAMLALAFDQAVMREDRGYFCLPEVDLGLPFTPGMNALITARLTPTVAH
                     EAMVTGRRYGGPDALAAGIVSEVAPEAEVLERAIARAEALAGKPRHALAAIKRGMYGP
                     VLDVLLP"
     gene            complement(9030..9464)
                     /locus_tag="VF517_00090"
     CDS             complement(9030..9464)
                     /locus_tag="VF517_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8051361.1"
                     /translation="MEDRTPAGAAFNDLVGQVVELNRRLTAAAETLTRPAGQTLARWL
                     VLAECRDEPATVADIARRLKLARQSVQRVADLLVDDGLCAYRDNPRHRRAKLLALTAR
                     GRATLGRIEAAQRDWADEVGGRVGERELRAAQKSLAAVLEQV"
     gene            9532..10629
                     /locus_tag="VF517_00095"
     CDS             9532..10629
                     /locus_tag="VF517_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX8051362.1"
                     /translation="MGQAELDDLAERLARTRWPDEMPGTGWDRGVPLGYLRELAEYWR
                     TSYSWRDQEARLNELPHFTTRIDGQRIHFVHVRSAREDAMPLLLLHGWPGSFVEFVDV
                     IEPLARDFHVVVPSLPGHCLSGPIDEPGWTDGRAAAAFLELMSRLGYERFGAQGGDTG
                     AFVAPEMGRRAPERLIGIHLNALVTMPSDDPDDLAALTDRERQRLEHHRRFRETEMGY
                     AQIQGTRPQTLAYALTDSPAGQLAWIVEKFKEWTNPAAELPEHAIDRDLLLTNVMLYW
                     VTGTARSSATSYYERFRDPAMWAPKEPSGVPTGVAVFPTDVAIRRFAERAHRIVHWSE
                     YEEGGHFAALEAPDVYVGDVRRFFERVRAVI"
     gene            10639..10947
                     /locus_tag="VF517_00100"
     CDS             10639..10947
                     /locus_tag="VF517_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051363.1"
                     /translation="MLVRIWRTRIDATRATDYDAFAEQRSRPMFERQEGIEGVVFARN
                     GADCAVITFWRDAAALEALAGSRSYSETVDALGATGILTGEASVEVLEVQPGSSAPPA
                     "
     gene            complement(10928..11842)
                     /locus_tag="VF517_00105"
     CDS             complement(10928..11842)
                     /locus_tag="VF517_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8051364.1"
                     /translation="MDSSRTATPAAAEQALRVNGVDLCVETFGALRDPALLLVGGASM
                     PMDWWHDGLCRTLAGAGRFVVRYDHRDTGRSTTYPPGAPGYGFDDLVADAVGLIDRLG
                     VERAHLVGISMGGGIAQRIALDHPDRVETQTLIATSPGLRPGARTDGDLPQRTPALLA
                     HFRDARPEVDWTDRDALIDLVVAGQRRLTGEGAFDATSVRRIAARVVDRSRDLRAAQT
                     NHGVIDPGEPYRSRLGRTRAPTLVVHGTADPLFPLGHAEALAREIPDAVLVRLDAVGH
                     QVPPPASWGTFIPALVWHVRDRVRLGVH"
     gene            11944..12315
                     /locus_tag="VF517_00110"
     CDS             11944..12315
                     /locus_tag="VF517_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8051365.1"
                     /translation="MKYLILIHHNQRSREAWEAYTEAERAAGIRAHVALVEDLAESGE
                     MVMSEALADPSHARRVHVRDGKTTTSDGPFAEVKEHLAGFYLVECETIDRAIEHAARI
                     PEAAFGNVEVRPVLSRNGVEM"
     gene            12312..13541
                     /locus_tag="VF517_00115"
     CDS             12312..13541
                     /locus_tag="VF517_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8051366.1"
                     /translation="MRTALRVEELLRELSPRVLAALVRRHGSFETCEDAVQEAMLAAI
                     DQWPRQGVPDNPHGWLVHVASRRWIEQWRSDSARARRERAVASLEVPAAAAQPEPAQE
                     DDTLTLLLLCCHPSLTPPSQVALTLRAVGGLTTAEIARAFLVPEATIGQRISRAKGRI
                     RASGGEFRLPPDSTLPERMAAVRHVLYLIFNEGYTASSGPALSRVDLTAEAIRLTRQL
                     LERLPDDGEVRGLLALMLLTDARRAARTDADGNLVPLSEQDRSRWDAELIAEGVELIS
                     EALASAPLGPYQLQAAIAAVHDEAADPGETNWPQILALYDLLRVVGPGPMVTLGRIVA
                     FAMVHGARAGLDELARSEAQVEEAAHHRVDAVRAHLLELTGDRRAARNHYELAARGTL
                     SIPEQRYLSKRAAALEV"
     gene            13543..14379
                     /locus_tag="VF517_00120"
     CDS             13543..14379
                     /locus_tag="VF517_00120"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8051367.1"
                     /translation="MQVIKTGIVLGESPRWRDGRLWFADWVAREIAAVDPEGNAEVIV
                     EGAGVLPICFDWLPDGRMLIVSGREGMLLRRELDGTVAPYVDLTELAPPPWNDIVVDG
                     RGNAYVGNTGFDFPEGEFAPGMLALVRPDGSARKVAGDVALPNGLAVSADNSTLVLAE
                     SYGRRLTAFDIADDGNLGGRRVWADLGDGVPDGICLDAEGAAWYGDVPNRRCVRVREG
                     GEVLETIELDRGCFACMLGGEDGTTLFMVAADWRGSTSMDGEPTGRVIATAASAPHAG
                     WP"
     gene            14385..15749
                     /locus_tag="VF517_00125"
     CDS             14385..15749
                     /locus_tag="VF517_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8051368.1"
                     /translation="MSERRQRAAVLGLMLGTLVAVLSSTVVATALHVIVTDLAGTQAD
                     YAWVITAALVTVTVSTPVWGKLADLVDRKVLLQAGLAIYAGASLAAGISGSTGELIAW
                     RAVQGVGVGGLTGLVQVVLSDLVPPRELGRYSGYLGGVLAAGSVSGPLLGGAIAETLG
                     WRWCFFLGVPIAVGVSAIVHRTLRLPRRRREVHIDVPGALLFCGGMTTLLIWVSLGGR
                     AYPWASWQTAALVLSGVLLSVAAILVERVAREPLIPLRLFQNRTVVIASVASVAVGTA
                     LFAASVFVPQYMQLARAKSPTESALLTMPLFAGLVIAAAVAGRTIVRTGLYKRWLVAG
                     AALLTAGAALMGSLDETTSLPFVGACLLALGAGIGMLMHSLTVVTQNAVGRRDVGAGT
                     SLVAFFRGIGGAFGVTVLSALFAGRAGAGTGHVFLAVAPIALAALVALALMRPVPLRL
                     RIDD"
     gene            15779..16819
                     /locus_tag="VF517_00130"
     CDS             15779..16819
                     /locus_tag="VF517_00130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEX8051369.1"
                     /translation="MGAVSTSPNSGRDAERSAVAAIRDGDEAAFVALAEHYRRRLQLH
                     CYRMVGSLEDSEDLVQEAILRAWRRRRSFEGRSTFQTWLYRIATNVCLDFLERRPVRE
                     LPAHYGLAIEPGSAVPEASELVWLDPYPERLLEGIASSQEDDPEAVLASKETIELAFL
                     AAIQHLPPRQRAVLIVRDVLGWSAKETAAALELSVPSVTSALQRARATLRERLPRQPL
                     EWSVGTAPSSDERALLERYIEAHERGDVDALAGLLRDDVRVAVPPLPGAREGREEFRA
                     SAMRSAAPGRFRCLPTRANGRPAAASYVLRDGAYRPLAIDVLGVEDGLLASITVFLRP
                     ELFAAFGLPDSL"
     gene            16894..17133
                     /locus_tag="VF517_00135"
     CDS             16894..17133
                     /locus_tag="VF517_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051370.1"
                     /translation="MLIEPDSRVAYDEADWEGALVVLERGAIELETLDGSRCGYAPGA
                     VLYLGRMPLRAIHNAGREVCILSALSRRHRPKEPR"
     gene            17130..17906
                     /locus_tag="VF517_00140"
     CDS             17130..17906
                     /locus_tag="VF517_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8051371.1"
                     /translation="MKTTQSADGTQIAYDELGEGPAVIMLAGAFNDRATTAPLAAALA
                     PRFTVLNCDRRGRGDSGDTQPYAVEREIEDIAALLDAAGGSASLFGYSSGAVLALEAA
                     AAGVPIDKLALYEPPFIVDDTRPRPPADLPEQVAALVEADRRGDAVELYQTKAIGLPE
                     EVVVQLRNAPFRPGLEAIAHTLVYDAILTRDLTPTTERFGKVTEPTLVLGGGESWEFL
                     RNGADALAEVLPNAERRTLPGESHDINPEATAEALGEFLA"
     gene            complement(17903..18853)
                     /locus_tag="VF517_00145"
     CDS             complement(17903..18853)
                     /locus_tag="VF517_00145"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8051372.1"
                     /translation="MTDRGHTDQRLLLASAQAGDERAFRQLVEPFRHALEVHCYRMLG
                     SSQDAEDLTQETLLRAWRALGRFEPRVQFQTWLYRIATNACLDELERRPRRPEPVDPF
                     PDRPADATAPPTYDPAARYAIREGMELALLRAIQELPGRQRAVLIFRDVLGWTAPEVA
                     ELLESTVASVNSALQRARGTIDPHLTEPASAEANPAEHQLLNRYVAAFEHDDIDGLIE
                     LLHEDAKLRMPPNPSLSGGAEIARFFLEIVAQGDLTRIRHRPTWANGRPAVTAELRGE
                     DGTLTPQGISVLEIEDGRIVGIEAFLDPALVPRFGVRPGR"
     gene            complement(18858..19610)
                     /locus_tag="VF517_00150"
     CDS             complement(18858..19610)
                     /locus_tag="VF517_00150"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX8051373.1"
                     /translation="MTVMTANEIVGREEWDAARDELLTREKEQTRLEDELAAQRRALP
                     WTPVEKGYTLETEAGPRTLVELFEGRSQLSMYHFMFGPDFTDGCPTNSSIADAFDPLV
                     PHLTARDVTMICVSRAPLDKLLAYRQRMGWSFNWASSYESEFSFDFGGSTRPQVREAG
                     LQAVPDGMPPIALRNAEACGVDVGTYISEGFAFATFARQGDDVYLTYSSGGRGVEFLM
                     TYYPVLDRTPRGRDEGEEFQMWLRRHDEYGGA"
     gene            complement(19672..20397)
                     /locus_tag="VF517_00155"
     CDS             complement(19672..20397)
                     /locus_tag="VF517_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051374.1"
                     /translation="MSLAIADKTVLVTGANRGLGRALVDEALSRGAARVYAATRQPMG
                     VTPERVTPLILDVTDAAQIREAAGAVDSLDVLINNAGVSVPDDLSDRSALEEHLAVNL
                     YGTLDVTQAFLPALVRSHGAVVNVVSLGAVSAVPVLPAYSVSKAASLSVTQSLRALLA
                     GRGVSVHAVLAGPIDTDMVRGLDIPKTPPEDVARATLEGVERGDEEIFPDPMSQSLAD
                     GWRAGVTKALERQNAAMVPAVAA"
     gene            complement(20488..21090)
                     /locus_tag="VF517_00160"
     CDS             complement(20488..21090)
                     /locus_tag="VF517_00160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX8051375.1"
                     /translation="MSETNTEHRLVIVVGSVREGRFGDTVAEWVAERAEEHGGFDVEV
                     VSLAKAGVGDPLPAVPPKFAGESYPRPPGMAPLTAALDEAQAFILVTPEYNHSYPASL
                     KAAIDWHFTQWTAKPVAFVSYGGAAGGRHAVLHLENVLTELHAVTIRDGLAFPNYFSA
                     WEDGRPLDPDAPAYATVVLDQLAWWADALRTARETAPYPA"
     gene            21201..22046
                     /locus_tag="VF517_00165"
     CDS             21201..22046
                     /locus_tag="VF517_00165"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8051376.1"
                     /translation="MGTPAGDFIRAKRDSLQPESLGLPERERRRAPGVRRSDLAARAG
                     ISVEYLTRIEQGRHRNPSASVISALADAMSLDPAESEHLRYLVKITGGACSGHERPEP
                     PSREVRPDVLATLRLLEPAVACVTNRLGDVLAWTDAFEVLMAEAGLLDSAEPNLTSYV
                     FTDPRAREFFPDWDRVADEQVFDLWLGPSAATSEWFRTELAPAAGKEFTKRFDRHVPP
                     PRAPLHVRHPGAGDLRWNRETLELSRSEAQQIVVFLPADDATTRAMEELSRRSRTGRA
                     LRAVT"
     gene            complement(22043..22693)
                     /locus_tag="VF517_00170"
     CDS             complement(22043..22693)
                     /locus_tag="VF517_00170"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8051377.1"
                     /translation="MSDDRPRRGRQAEALRNDTLVLDAARAVFAEGGADAPIAAVAER
                     AGVGMGTLYRRYGGKDELLQKLCLLSLEQNLAAAAGALAAATAWEGLCDYVRTCVGLA
                     VGAFGPLAGEIEATAEMTRLARAVRRRVTALAGRAQEAGDLRADANAIDVLQLIERLS
                     RAFPPSGDPAEEATRRRQVEIALAGLRAGAREPLPGPAPRPGDYERRWTRPRPAAS"
     gene            22770..23735
                     /locus_tag="VF517_00175"
     CDS             22770..23735
                     /locus_tag="VF517_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8051378.1"
                     /translation="MRAAVVRDFGGPEVLSVEAVPDPAADPGELVVEVEYAGITFVET
                     QVRAGRPPNPAMLPHLPFVPGNGVAGRVAEVGEGADRGLLGRAVVTTTGGSGGYAERV
                     AVPAALPIEIPEGVSARDAVALLADGRTALALARVAAVEAGDVVLVEAAAGGVGSCLV
                     QLARAAGATVVAGVGSVHKVPIARDLGAGRVVDYSRDGWEDEIRTELGGLDVVFDGVG
                     GAIGASALTLLRPGGRLCQYGMASGSFTNVPAERRDVQVLRGTALTPAESRELSIEAL
                     RLAAAGELSATVGQEYPLERAAAAHAAIEARSTIGKTLLRVARIS"
     gene            complement(23742..>24198)
                     /locus_tag="VF517_00180"
     CDS             complement(23742..>24198)
                     /locus_tag="VF517_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051379.1"
                     /translation="TAEQNGPVSLDLFRETFATNVLGAVAAIEAFLPLLTKAEHARIV
                     NVSSTMGSLADQGDPESPYYSVVVPAYQSSKAALNGITVALSKALEDAGIAIYSVCPG
                     FVQTDLTPMNRDNAPLTPEDAAETVARFALSEDASLSGQFLSSAGPVPW"
BASE COUNT         3418 a         8448 c         8673 g         3659 t
ORIGIN      
        1 gttgacgtcc ccgaccgcgc cgaccgcgaa gccgggcgcg ccgtaggcct cgtcccacgg
       61 cgggtagtcg atgtgctggt cgccgttggg gccgagcaca tcggcgggaa ccgtcgaggc
      121 ggcggcgctc gccgcgagca cgccgagcgt gagcagcgtc gcccccagtg ccccgatccc
      181 ccgccccata gccctcaatg ctcccaccgc ttgtgcgaac acgccagtgg ttcgactgga
      241 gcggtggtgt cttgcgctgc tggtgccggc ctgagcgccg gctgccaacg gcgggccgcg
      301 cccgcgctag attccgtagc gccctggtgg gcgtagctca gctggtagag ctcctggttg
      361 tggtccaggc ggtcgcgggt tcgagtcccg tcgctcaccc ttcgagaaag ccccgcaaac
      421 tcgcgtttct actttcgagt actcgcgtgg agttcgcaga ggcatcaata gcgtccatct
      481 gctcgcggca tggcccactc ggcgctgcct tgacggtcct acggttgacg ggtctggtcg
      541 gcggcgatcc tttgatcctc cgccggggag cacgaccggt cctactaaag acctatacca
      601 aggcaggtca ggtgactgca gaccgccggt gcacgcatgc gcatgggacc tgcggcgtgg
      661 ggagagcgtt gggttcctag ccaagaaccg cggtactggt tatccactcc agccgtagac
      721 ggccgcccgt aggccgttcg gggtcgagac gttcggagct ccggctgggc aggagttgct
      781 cgcggtgaag ttgccgcctc cggtaggctg gctccccgtt gtgtagacgg tgatgatctt
      841 gccgaacgtc gcggtgaacg agatcgagag cgaaacttgg tcgccgcccc taaccggcac
      901 ggccgagaag ctgaactggg tgtccccgcc tgccgcggac gccgcctgtg cgcgcagggt
      961 gccgttcacg tagacggcca ggtgaccagt gaccgatccg tcggggtcga tctgcacgag
     1021 cgccgagtcg atcgacgcga ccccggaggg caccgtgaag gtctgtgtcg cggtcccgcc
     1081 gggcatcgaa gccgagttgc cgggattgcc gcggcacatc gcgtgaccag cagcggccgg
     1141 cttgtagtgg tcgtacgcgc tgacccgcac gggtgggggc ggcggccgtg gttgcggttg
     1201 gggctgtggt tgcggttggg gctgctccga cgctggcggt ccgcccgact ggggtccgcc
     1261 gacatccgat cggcctggcg gcgcaaccgt gctcgtcggc cgaatctgga tcgtcacgtc
     1321 agtcggcgtt ggaagctcgg cggcggacac gctcagcccg aggctgcggc catcgcgcgg
     1381 aaccgggagg gtgacgacat cgaaggtccg cccctcgacg acattcggct gcaggcgcga
     1441 ctgaccgggg cctccatcca ggtcggcgat gaccctgcca ttccggttgc ggacgtcagc
     1501 ggaggcaagg tcgacatcca cgtcgtccgt accccacgag accacaacct cgacggcggg
     1561 ggcgcctgac gcgagaagcc cgcggaccgt ggaacttggc aaccccggtt cggcgagcct
     1621 gaccggggtc gttgcgtccg acttcgcgca ggtcagctgg gcgtcgatct cgttgacgac
     1681 gccctgcagc cgcgcgacct gcgtcgcggc gctgtcgtcg tcttggagct tcagggggaa
     1741 gtagcgtccg cccgtctcgt cggcgatctg caagaggcgt tcggcgttct cgttgccctc
     1801 accgccgggg ccgatgtcga ggccgatcac gtgcgtcggc ggtccaccgg cgtgcaactc
     1861 ctcgtattcg ccgtcgttgt gggcgccgtc ggtgaggaag atgcgtgacg tcgcaccggg
     1921 ctggtgctca gcggttgctc ggaacgcgtc gttgtagtcg gtcccgccgc tgtcgttctc
     1981 gccgatcccg tcgtcggcca acgccgcgag cgcctcgagc atccgcggcc gcgagtcggc
     2041 gacgagcccc ggctcgaaca gcgatccggc gtctccggcg aactcgactg caccgaaggt
     2101 tcgcatctgc gccgcaggct tggacaccag aagctcgagc gcgcgccgcc tgatgccctc
     2161 agggtcgcta ccggccatcg aacccgagtc atcgacgatg gcggcgacgt tgtcagacgg
     2221 ggtgcaaggc gcctggtggg cgttgatcca ctccaggacc cgcttcgcca tcgcgagctg
     2281 gcccagctga ttcggatgcg cctgctcctg cccgtccttc aagttgatct tccgcaccca
     2341 cgaggccgag tccctagtgc agagctcgtg ccccttgagg acatcggcga gcgacacgaa
     2401 gtcgacgccc gccgtggacg cggcgctcgc gaatgttgca tcaagggtct taatgagctc
     2461 ggcggcgcgc ttgcggtcgc gcttgtcagc ccagcagccc tcaatcttct tgtccgccga
     2521 cggcacgatg ttgggatagc cgacgacaag tagcgacgcg ttcgcagcca gccggacctc
     2581 cttgtaacgc tggatcagct gcggcttcac gcgcttctta agatctcgct tgattgccga
     2641 gaggttgttc aggcagaggc gcgtgtggac aaagcaatcg cgtatgcgac ttgcgaatcc
     2701 gatgtcgttt ccacccatcg tgagcagtac gcgatcgaac cgcgtctcgg ctgcgagatt
     2761 ccgcaggcgc tcgatctgcc cgatgtcgtc cggcggcttt tccgcctgtc cctcgacaag
     2821 gcttttgatg atcgcgccgg agcacgcgag gtggtgatcc cgcgagactc cgagcaggcg
     2881 cggccacgcg ttcccgctgc gatggcagcg gtgcttgcgc gggaatccca cgtcgtcgtc
     2941 cgtcccttcg tcgtacgagc cggccccctc accggaggag aacgagtcgc cgagcgacac
     3001 gagggtttca gcggacgcct gagccgcgcc tagggcaagg gcgagcgagg cgagcacgaa
     3061 ggctgccgaa agccgcaggc tgcgattcca attgagggcc accacgttgc gagtccgatg
     3121 cagcgaggtc tcccccgccg ccgacgaacc tatgcgcgtt ggcgcattga cgcaagcccc
     3181 cccaggcggg ggcaccggta gcggtcagcg taattcgagt tgacaaaccg acgcctgcag
     3241 cagagggtga gcgggcaggc gctcaacgat ggatggttcg agcgatcaca gccatggttg
     3301 gcgtgggact cgcagggtgt cttgacgatg cgctttccat ggcgggccgc cgcggccgct
     3361 cggctttagc tgcaacggag ggagcgatgg cgtcgtgtgc aagaagggca ttcgccgcgt
     3421 tacctggggt tattactacg actgatcgga tgggcatgag aacgaggacg tcaatctgtc
     3481 ggcgcccggg gcgcctcgca gcggtggcag cgtgcgctgc gattgccctt gcggttggtt
     3541 ttgggacggg cggtgggatc gcgatcgcgg gccccgtcgc cgcgtacgac tggccgggca
     3601 tgaagaagtg cggatccttc aaggcgaagg ggtaccggat ctacgtttac gcgaataagc
     3661 ggctttcgtg cgagcgggcc aggcgcgtca tgaaggcacg ctggggtccg tcgagtggga
     3721 tcgtcacgca taacggtggg accggagcga gcgggtggat aacgctcaag aagtatcccg
     3781 gatggaagtg ctactcaggg gccggagggg gcacgtgccg caagagcaag gcagtagctg
     3841 gctaccagag ccgggtctga cgccaagctc cacggcgtcc cctccccttg gttaggacgc
     3901 taggcccgcc tagcatttgc ccatcgcgaa tcgcatccgc cggaactttg cactctcggt
     3961 gttcgccgcc gcacttgtcg cggtgggggg gtgcggtgcg gatgacacca agcccgtcaa
     4021 cctcgacgac tcctcgcgcg agccgcgcaa tctcgacaag cgcatcgagg acgccgagcg
     4081 gcggctgagg cgcctgcaag aagggcggag cgagccccgg gcgccgcgca cgacagtggg
     4141 tcttcggggt gcggaggatt tccttgcgtc gctgaatgcc aaggctgggg tcaccgctgg
     4201 cccacccggg gccgatgagc ccgagacggc tggtgatctg acgagcggac cggcctggtc
     4261 gaccatcaag gttccgattg cactgcgcgt gctcgaccgg gctggtgggg tgagcgccct
     4321 tggcgatacg aagcggagtc agatcgaaag cgcgataacg agatccgaca acggtgcggc
     4381 agcccgtctg ttcgcgagcc tggggagtac ggcggaggcc gctgccgatg tccaagatgt
     4441 ccttcgtcag gccggcgacg acgagactca cgtctcgaca gagggtcgcg gagagttctc
     4501 accatacggc caaaccgatt gggcgctcgc gcggcagcac cgcttcatgg cggcactcgc
     4561 tggaggctgt ctgggcgaca agaacaacgc cgactacctg ctctcattga tgggcgcagt
     4621 tacctcagat acatgggggc tcggctctgc tggcggcccc gcgaagtgga agggcggatg
     4681 ggggccggga ccggaggggc gttacctcgt ccggcagatg ggcgtcatcg agttcggttc
     4741 ccggaagctc gtggtcacgc tggccgccat accccacgac gggcagtttg cgaccggaca
     4801 gcgcgtcgcg accgccatcg cggagtggct gcgccggcac gcgcgcggcc tcgccgggcc
     4861 ggttaatcgc tgctgacgcg ggtccccgat tcggggactg gcgcattcgc acgagtgcag
     4921 ataggttgcg cacaatggtt cagattcgtc gagcactaat tgggagtctc tgcctgctgg
     4981 ccatctcgtc gggtattgtc gcctgcggtg gcgacaacgg tcccagtgat gaagagatac
     5041 gcacggcgcg cgaggagggc gagaaggccg gtcgcgatcg cgagcggctg cgcgatctag
     5101 agcgcaagct tcgtgagcag gagaagaggg acccgggcgg tggccgctcg agtcccccga
     5161 gtagcggggg tcaacccgca ccgcggcgca ccaactgcgg tagcgacttg caggccgggc
     5221 cgaacacttc gtgcgcgttt gcgggcaacg tacgggagga gtactactca gcaggaggca
     5281 gtggatcgat gagcgtgacg gtctacagcc ccacaaccga tcgcacgtac accatgtcct
     5341 gctcggctgg cactccgcac gtgtgcaccg gcggcaataa cgcctccgtc tactttccgt
     5401 gattccctcg agcggacgtg tattcgcagc cctcgcggtg atgttggcga tgtcggcatg
     5461 gtctccgctc ggtgcgacgg cggatgcgaa gagctggcga gtggagcttg acggggacac
     5521 tgcgcgcgag actgtcgcga tcaagaagcg ccgctgcaag atgccctact acgggtgcac
     5581 gcaggtggtc cttcaggatg caagcggcgc aaggtgctga cgcccatcag ccagcggccg
     5641 aggtttccgt attactggcg cgtgacaaag gtcaggttcc gcgacctcac cggcgacggg
     5701 ctgccggaga ttgtctggga gctgttcacc tccggcggga ccggtagctc gccatccctg
     5761 aagggcgtcg accgatggaa cggacagagc gcggcccgcg tcttccgctt tcacaacgtc
     5821 gaccggaaac cgcccccgga ctttgagtac gtcatcggcg tgagttggcg gatcgtcggc
     5881 gcggtcgacg gtggcctagc cgagatcgag acctccgagt cactgcaccg atccgacgac
     5941 gggacctgct gcccgagcgc gtaccgagtg acacgccacc ggtgggacgg gacaacgatt
     6001 gcgccagtct cagggtctga gacgatcgag gaccagtaga cagcgtcggg cgcgccagga
     6061 cggatcaagg ctgtttctga gcaggatcgt cgagtcccgt cagcgttgcc gacgaccccg
     6121 tcgcgagctc ccgtgctcag cgcgatctca cgaggccgtt tccggcgcga tcagcggggc
     6181 aaccgcggga gtacgcggct agctgaccga ccgcgactgg cgcccaccct cgagccccca
     6241 ggagacgatc cgctcgggat ggacgcggat cgtcgggcgt ggctcgcgga tcgcctcggc
     6301 tcttccgcgc acctccacgg cccgcggtcg ccagggatta gtgctggcga ggtcgtcgat
     6361 cacgatcgcc gcccggccgg agcgctgcgc gtcgcggaac ttcttcgtgg cggccaggtc
     6421 gcggccggtg acgtcgatcg tgtcgtgctc ggggttgtac gaccagccaa ccggcacgac
     6481 gtggggcgtg ccgtcggcgc cgaccgtggc gatgcggccg agtcgacgac cggaccggag
     6541 gtagtcgagc tcgccgtcgg tgaatctgct catctcagcc cgctcccacc ggcgtcgact
     6601 ggtcggcgat cgcgaggtcg tacacctcgt accgggttcg cagcggaggc gagacgcgat
     6661 cgcccatgac ctcgcgcacg gcggggtgga ggtgctcctc gtcgaagcgc tcgaagtcct
     6721 cgtaggtctc ccacacgtcg aacacccgga gcccgccctc gccgcgctcg ccgacgacgt
     6781 ggacgaggtg gccgggcgcc agcgcgccct tgccgccgag cctctccgtc accgcctcgt
     6841 acatctcgac ggtcccctcc gggacctcgc tgagaatcgc gactgccacc gtgccctcct
     6901 tcctcgtgct cacgcggcgc gcgagagctc gtcgccgggc ccgccgtcgc tgccggtcgc
     6961 gcgccgaacg agctcgccgg ccacgccggc gagctgcccg cgcaggagcg cgtcgcgcag
     7021 gtcgcccgcg gcgtcgaact gctcgtgcac ccttccgacc ggcacctccc ggtcgatgac
     7081 gtcggcgccg caggtcttca ggaccttgcg gagctcggcc tgggcccagg cagccccgaa
     7141 cgggctccgg ctcgcgccca cgaccgcggc cggcttgtcg cgcagcgagt tgtcggggaa
     7201 cggccgtgac gcccagtcga tcgcgttctt gagcgcgccc ggaatcgacg agttgtactc
     7261 cggagtcgcg atcaggagcg cgtccgcgtg ctcgatcgcc tcgcggaggc gccggaccgc
     7321 tggcggcgcg ggctccacgt cgatctcctc gctgtagggc ggtagctcac cgatgccctc
     7381 gtagagctcg agctcggcac cgttcgggag tgcgcttccc acggtgtcga ggagcttcct
     7441 gttgtacgag ccacctcgaa ggctgccgca gagtccgatg atcctcatct tccgtcacct
     7501 cctcttaggg ttaggtgaca gtatcatgta aatacatgaa taagtcaact atccggtcga
     7561 agaagcactc gagtgctcgg ccgaagaagc tctcgagggc tcgcggcgag ctggtcccgg
     7621 tctccgggtc gttcgtgacc cgccaacccg cggaaccgca ggggccaggc cggcgcgccg
     7681 ctgcagctag aacgcctcga gcgccgagtc caggctcgga gccgggcgac gctggggggc
     7741 gggcgcgttc gacgcgtcga gcgtgttggc gatctcgttg agcttgagcg cgagctcgcg
     7801 ctgctcgggc tccgaccagg tgccgaaaag gcgctgggtc tccgcgccga gcctctcggt
     7861 gaagcgatcg acgagctcct gcccctcgtc gctgagctcg accacccgca tgcgcccatc
     7921 accttcggcg tcacgacgcg cgacgaggcc gcgccctgcg acgcggtcga ggagccgact
     7981 gacattcgag cggtcgcagc tcatgatgtg cgccagctcg ccgacggacg ccctgcgctg
     8041 cgccaggcag aggagctccg cctgctgcgc ggtgagcccg agctcccggc ttacgcgcgt
     8101 gtatgtggcg ttcatcgcca gcatcacgtg gacgagccct cgagtcaggc tgacgtcctc
     8161 cggggatcgc gcgcgctcgt tcatgggcac gatcctaatg gaagtagttg attaaggcac
     8221 cgagttgaat ggcatgtaca tatgctttgc tgaagacatc acggaacggc ggcgacggca
     8281 ggcggaggta gcgcggatga tcacaccagg aggcgcagac cggctggacg tcgagcgccg
     8341 cggctcggtg acggtggtcc ggatgacgca cgacgagaac cgcttccatc ccgagctcct
     8401 ggcggggatc gaagcggtcc tcgacgaggt cgagttcacc gaggacgccg gcgccctcgt
     8461 cgtcaccgga tccggcaagt tcttctcgaa cggcctcgat ctcgagtaca tgtcgagtca
     8521 cccggacgag gtggacgagg tcctcgcacg ggtgcacgcg ctgttcggcc gggtgctcgg
     8581 cctcgaggtg ccgacggtgg cggcgatcaa cggccacgcg ttcgccgcgg gggccatgct
     8641 cgcgctcgcg ttcgaccagg cggtgatgcg cgaggaccgc ggctacttct gccttcccga
     8701 ggtggacctc ggtctgccct tcacccccgg aatgaacgcc ctgatcaccg ctcgcctgac
     8761 gccgaccgtc gctcacgagg cgatggtcac cggacgccgc tacggaggcc cagacgcgct
     8821 cgcggccggc atcgtctcgg aggtcgcccc cgaggccgag gtgctcgagc gcgcgattgc
     8881 gcgcgccgag gcgctcgccg gcaagccccg ccacgccctg gcagcgatca agcgcggcat
     8941 gtacggcccg gtgctcgacg tgctgctgcc gtgagcagct gcccgaatcc gccatagcat
     9001 cgatcggcga cgcggaggcc gcggacccgt cagacctgct caagcaccgc ggccagcgac
     9061 ttctgcgccg cccgcagctc ccgctcgccc acgcgcccac cgacctcgtc ggcccagtcg
     9121 cgctgcgccg cctcgatgcg ccccagcgtc gcgcgtcccc tcgcggtcag cgcgaggagc
     9181 ttcgcgcggc ggtggcgcgg gttgtcacgg tacgcgcaga gaccgtcgtc gaccagcagg
     9241 tcggcgactc gctgcaccga ctggcgtgcg agcttcagcc gccgcgcgat gtcggccacg
     9301 gtcgccggct cgtccctgca ctccgccagc acgagccagc gcgcaagcgt ctgccccgcc
     9361 ggccgcgtga gcgtctcggc cgccgcggtc agccggcggt tcagctcgac cacctgcccc
     9421 acgaggtcgt tgaacgcggc gcccgccggc gtgcggtcct ccatattgac agcatcctgt
     9481 cgttcttcta atcttcgagg catcgacagc atcctgtcat attgcatcga agtgggccag
     9541 gcggagctcg atgacctcgc cgagcgcctg gcgcggaccc gctggccgga cgagatgccg
     9601 ggcaccggct gggaccgcgg cgttccgctg ggctacctgc gggagctggc cgagtactgg
     9661 cggacgagct acagctggcg cgaccaggag gcgcgcctga acgagctgcc gcacttcacc
     9721 acacgcatcg acggccagcg catccacttc gtgcacgtgc gctcggcccg cgaggacgcg
     9781 atgccgctcc tgctgctgca cggctggccg ggctcgttcg tcgagttcgt cgacgtgatc
     9841 gagccgcttg cgcgcgattt ccacgtggtc gttccgtcgc tgcccggcca ctgcctgtcg
     9901 ggccccatcg acgagcccgg ctggaccgac ggccgcgcgg cggcggcctt cctcgagctc
     9961 atgagccggc tcggctacga gcgcttcggc gcgcagggcg gcgacaccgg cgcgttcgtc
    10021 gcgcccgaga tgggacgccg ggcaccggag cgcctgatcg ggatccacct caatgccctc
    10081 gtgacgatgc cgagcgacga cccagacgac ctggcggcgc tgaccgaccg tgagcgccag
    10141 cggctcgagc accaccggcg gttccgggag accgagatgg gctacgcgca gattcagggg
    10201 accaggccgc agacgctcgc atacgccctc accgactcgc ccgccgggca gctcgcgtgg
    10261 atcgtcgaga agttcaagga gtggacgaac cccgccgccg agctgcccga gcacgcgatc
    10321 gaccgggatc tgctgctcac gaacgtgatg ctctactggg tcacgggcac cgcgcggtcg
    10381 tccgcaacgt cctactacga gcgcttccgc gaccccgcga tgtgggcccc caaggaaccg
    10441 tcgggcgtcc cgaccggggt agccgtgttc cccacggacg tggccattcg ccgcttcgcc
    10501 gagcgggccc accgcatcgt ccactggtcc gagtacgagg agggcgggca cttcgcggct
    10561 ctcgaggcgc cggacgtgta cgtcggggac gtgcggcgct tcttcgagcg ggtccgcgcg
    10621 gtgatctagc ttgaccgcat gctcgtgcgg atctggagga cacgcatcga tgcgacgcga
    10681 gccacggact acgacgcctt cgccgagcag cggtcgcgac cgatgttcga gcggcaggag
    10741 gggatcgagg gtgtcgtctt cgcgcgcaac ggagcggact gcgcggtgat caccttctgg
    10801 cgcgacgcgg cggcgctcga ggccctcgcc ggctcgcgct cctacagcga gacggtcgat
    10861 gcactcgggg cgacagggat cctgaccggc gaggcgtcgg tcgaggtact ggaggtccag
    10921 cccgggtcta gtgcaccccc agcctgaccc ggtcgcgcac gtgccacacc agcgcgggga
    10981 tgaacgtccc ccagctcgcc ggcggcggca cctggtgccc gaccgcgtcc agccgcacga
    11041 ggacggcgtc cgggatctcg cgcgcgagtg cctcggcgtg cccgagcggg aacagggggt
    11101 cggccgtgcc gtggacgacg agcgtcggcg cccgcgtccg gccgagccgc gaccggtacg
    11161 gctcgcccgg atcgatcacg ccgtggttgg tctgagcggc gcgtaggtcc cggctgcggt
    11221 ccacgacgcg agcggctatg cggcgcacgg atgttgcgtc gaaggccccc tcacccgtga
    11281 gccgccgctg cccggcgacg acgaggtcga tcagcgcgtc gcggtcggtc cagtcgacct
    11341 cgggccgcgc gtcccggaag tgtgcgagca gcgccggcgt cctctgtggc aggtccccgt
    11401 ccgtccgtgc gcccgggcgc agccctgggc tcgtggcgat gagcgtttgc gtctccactc
    11461 gatcggggtg atcgagcgcg atccgctgtg cgatcccgcc acccatcgag atcccgacga
    11521 ggtgggcgcg ctcgaccccg aggcggtcga tcagtccaac cgcgtccgcg accaggtcgt
    11581 cgaacccgta gccgggggcg ccgggtggat aggtggtcga gcgcccggtg tcgcggtggt
    11641 cgtagcgcac gacgaagcgc ccggctccgg cgagcgtccg gcagagcccg tcgtgccacc
    11701 agtccatcgg catcgatgcg cctccgacga ggagcagcgc ggggtcgcgc aatgccccga
    11761 acgtctcgac gcacaggtcg acgccgttaa ctctgagcgc ctgctccgcg gctgcggggg
    11821 tcgcggtccg tgacgagtcc atcgggctcc tagctttgat tcggcgaatg gctgtggagc
    11881 gaatgtcgaa accgagcagg ctgtttcgac accgatgaca cccgaccgtt gtctggaggc
    11941 cacgtgaagt acctgatcct gatccatcac aaccagcgct cgcgcgaggc gtgggaggcg
    12001 tacaccgagg ccgagcgcgc cgcgggcatc agggcccacg tggcgctggt cgaggacctc
    12061 gccgaatccg gcgagatggt gatgtccgag gccctcgccg acccctcgca cgcgcggcgt
    12121 gtccacgtgc gcgacggcaa gaccaccacc agcgacggcc cgttcgcgga ggtcaaggag
    12181 cacctggccg gcttctacct ggtcgagtgc gagacgatcg accgtgcgat cgagcacgcc
    12241 gcccgcatcc ccgaggccgc cttcgggaac gtcgaggtca ggcccgtgct gagccgcaac
    12301 ggggtggaga tgtgaggacg gcgctccgcg tcgaagagct gctccgggag ctctccccgc
    12361 gcgtgctcgc cgcgctcgtc cgccgccacg gcagcttcga gacctgcgag gacgccgtcc
    12421 aggaggcgat gctcgccgcg atcgaccagt ggccccgcca gggggtcccc gacaacccac
    12481 acgggtggct cgtccacgtc gcctcgcgga ggtggatcga gcaatggcgc agcgactcgg
    12541 ctcgcgccag gcgcgagcgc gccgtcgcct ccctcgaggt cccggccgcg gcggcgcagc
    12601 ccgagcccgc gcaggaggac gacacgctca ccctcctcct gctgtgctgc catccgtcgc
    12661 tcacgccgcc gtcccaggtg gccctcactc tgcgcgccgt cggcgggctg accacggccg
    12721 agatcgcccg agccttcctc gtgcccgagg ccacgatcgg ccagcgcatc agccgcgcca
    12781 aggggcggat cagggccagc ggaggcgagt tccgcctgcc gcccgactcg accctccccg
    12841 agcgcatggc cgccgtgcgg cacgtgctct acctgatctt caacgagggc tacaccgcca
    12901 gctccgggcc cgcgctgagc cgcgtcgacc tgaccgcgga ggcgatccgc ctcacgcgcc
    12961 agctcctgga gcgtcttccc gacgacggcg aggtgcgcgg tctgctggcc ctgatgctcc
    13021 tcaccgacgc acgccgggcg gcgcgaacgg acgccgacgg caacctggtc ccgctgtccg
    13081 agcaggaccg cagccggtgg gacgcagagc tcatcgcgga gggcgtcgag ctgatcagcg
    13141 aggccctggc ctccgcgccg ctcggcccat accagctgca ggccgcgata gccgcggtcc
    13201 acgacgaggc ggccgatccc ggcgagacca actggcctca gatcctcgcg ctctacgacc
    13261 tgctccgagt cgtcggcccc gggccgatgg tcacgctggg ccggatcgtc gcgttcgcga
    13321 tggtccacgg agcgcgggcg ggcctcgacg agctcgcgcg ctcggaggcc caggtcgagg
    13381 aggcggcaca ccatcgggtc gacgccgtgc gcgcccatct gctggagctg accggggacc
    13441 ggcgggcggc gcgcaaccac tacgagctgg ccgcgcgcgg aacgctgagc atcccggagc
    13501 agcgctacct gagcaagcgg gccgcggccc tggaggtgtg acgtgcaggt gatcaagacc
    13561 ggcatcgtgc tcggggagtc gccgcgctgg cgtgacgggc ggctgtggtt cgcggattgg
    13621 gtcgcgcggg agatcgcggc cgtcgacccc gagggcaacg ccgaggtgat cgtcgagggc
    13681 gccggggtgc tgccgatctg cttcgactgg ctgcccgacg gccgcatgct gatcgtctcg
    13741 gggcgcgagg ggatgctcct gcgcagggag ctcgacggca cggtggcgcc gtacgtcgac
    13801 ctgactgagc tcgcgccccc gccctggaac gacatcgtcg tggatgggcg cggcaacgcg
    13861 tacgtcggca acaccggctt cgacttcccg gagggggagt tcgcccccgg catgctcgcg
    13921 ctggtgcggc ccgacggctc ggcgcggaag gtggccggcg acgtcgcgct gccgaacggc
    13981 ctcgccgtca gcgccgacaa ctcgaccctc gtcctggccg agtcctacgg ccggaggctc
    14041 acggcgttcg acatcgccga cgacggcaac ctcggcggga ggcgcgtctg ggccgacctg
    14101 ggcgacggcg tgccggatgg catctgcctc gacgcggagg gggcggcctg gtacggcgac
    14161 gtccccaacc gtcgctgcgt gcgcgttcgc gagggcggcg aggtcctgga gacgatcgag
    14221 ctcgaccgcg gctgcttcgc gtgcatgctc ggcggtgagg acgggacgac cctgttcatg
    14281 gtcgccgccg actggcgcgg ctccacgagc atggacggcg agccaaccgg cagggtgatc
    14341 gcgaccgccg ccagcgcgcc gcacgccggt tggccgtagc ctcgatgagc gagcgccggc
    14401 agcgtgcggc cgtgctcggc ctcatgctcg gcacgctcgt agccgtgctg tccagcaccg
    14461 tcgtggccac ggcgctccac gtgatcgtca ccgacctcgc ggggacacag gcggactacg
    14521 cctgggtgat cacggcggcg ctcgtcaccg tcaccgtgag cacgcccgtg tggggaaagc
    14581 tcgcggacct ggtcgatcgc aaggtgctgc tccaggcggg tctcgcgata tacgccggtg
    14641 cgtcgctcgc cgccggcatc tccggctcga ccggggagct gatcgcgtgg cgggccgtcc
    14701 agggcgtcgg cgtgggcggg ctgaccggcc tggtccaggt ggtcctctcg gacctcgtcc
    14761 ccccaaggga gctcggccgc tacagcggct acctcggcgg ggtgctggcc gccggctcgg
    14821 tgagcgggcc gctgctcggc ggcgccatcg cggagacgct cggctggcgc tggtgcttct
    14881 tcctcggggt cccgatcgcg gtcggggtgt cggcgatcgt gcaccgcacg ctgcggctcc
    14941 cgcgtcgccg gcgagaggtg cacatcgacg tgcccggcgc cctcctgttc tgcggcggca
    15001 tgaccaccct gctgatctgg gtctccctcg gcggccgcgc gtacccgtgg gcctcgtggc
    15061 agacggcggc cctggtcctc tccggcgtcc tcctctccgt ggcggcgatc cttgtcgagc
    15121 gcgtcgcgcg tgagccgctc atcccgttgc ggctcttcca gaaccggacg gtggtgatcg
    15181 cctcggtcgc gagcgtcgcg gtcggcacgg ccctcttcgc cgcctcggtg ttcgtgccgc
    15241 agtacatgca gctcgcgcgg gcgaagtcgc cgacggagtc ggcgctgctc acgatgccgc
    15301 tgttcgccgg gctcgtgatc gcggccgcgg tggccggccg gaccatcgtg cgcacgggcc
    15361 tgtacaagcg ctggcttgtc gcgggcgcgg cgctgctcac ggccggcgcc gcgctcatgg
    15421 gctcgctcga cgagacgacg agcctcccgt tcgtgggcgc gtgcctcctg gcgctcgggg
    15481 ccggcatcgg catgctgatg cacagcctca cggtggtcac gcagaacgcg gtgggacggc
    15541 gcgacgtggg ggcgggcacc tcgctggtgg cgttcttccg agggatcggc ggcgccttcg
    15601 gcgtgaccgt cctgagcgcg ctgttcgccg gccgagccgg agccgggacc ggccacgtct
    15661 tcctcgccgt cgcgccgatc gcgctcgcgg ccctcgtcgc gctggcgctg atgcgtccgg
    15721 tcccgctccg cctgaggatc gatgattagt ttttcgccgc cgaccggtct taagggatat
    15781 gggtgctgtt tccacttccc ccaactccgg ccgtgacgcc gagcgctccg cggtagccgc
    15841 cattcgcgac ggggacgagg ccgcgttcgt cgcgctggcc gagcactacc ggcgccggct
    15901 ccagctccac tgctaccgga tggtgggctc gctcgaggac tcggaggacc tggtgcagga
    15961 ggcgatcctg cgcgcctggc gcaggcgccg gagcttcgag ggccgttcga cgttccagac
    16021 gtggctgtac cggatcgcca cgaacgtgtg tctcgacttc ctagagcgac gcccggtccg
    16081 cgagctgccg gcgcactacg ggctcgcgat cgagcccggt tcggcggttc ccgaggcgtc
    16141 ggagctcgtg tggctcgatc cgtatccgga gcgcctgctg gaggggatcg catcgtcgca
    16201 agaagacgac ccagaggccg tgctcgcctc gaaggagacc atcgagctcg cgttcctcgc
    16261 cgccatccag cacctgcccc cgcgccagcg cgccgtgctg atcgtgcgcg acgtgctcgg
    16321 ctggtcggcg aaggagacgg ccgcggcgct cgagctgagc gtcccctcgg tgaccagcgc
    16381 gctgcaacgc gcacgcgcga ccctccgaga gcgcctgccc aggcagccgc tggaatggtc
    16441 ggtcgggacc gcgccgagca gcgacgagcg ggctctgctg gagcgctaca tcgaggcgca
    16501 cgagcgcggc gacgtcgacg cgctcgccgg cctgctgcgt gacgacgttc gcgtcgcggt
    16561 gccgccgctg cccggtgccc gcgagggccg ggaggagttc cgggcctccg cgatgcgctc
    16621 ggccgccccc gggcgcttcc gctgcttgcc cacgagggcg aacggccggc cggcggcggc
    16681 gagctacgtg ctccgcgacg gggcctatcg ccccctcgcc atcgacgtgc tcggcgtgga
    16741 ggacggactg ctcgcgagca tcacggtctt cctccgcccg gagctgttcg cagcgtttgg
    16801 actgcccgat tcgctctgat ggaccgagcc gggtcgcgga caccggtctc gctcctgcgg
    16861 cacgagcccc cctcgtgggc ccatctccgg ctgttgctga tcgaaccgga cagccgggtc
    16921 gcctacgacg aggcggactg ggagggcgcg ctcgtcgtgc tcgagcgggg cgcgatcgaa
    16981 ctcgaaaccc tcgacggcag ccggtgcggg tacgcgccgg gcgcggtcct gtacctcggc
    17041 cgcatgccgc tgagagccat ccacaacgcc ggccgtgagg tgtgcatcct gagcgcgctc
    17101 tcgcggcggc accgaccgaa ggagccacga tgaagaccac tcagtcagcc gacgggacgc
    17161 agatcgccta cgacgagctg ggcgagggcc ctgcggtgat catgctcgcc ggtgcgttca
    17221 acgatcgcgc caccacggca ccgctcgcgg ccgccctcgc gccgcggttc accgtcctga
    17281 actgcgaccg ccgcggccgg ggcgacagcg gcgacacgca gccgtacgcg gtcgagcgcg
    17341 agatcgagga catcgcggcg ctgctcgacg ccgcgggcgg atcggcatcg ctgttcggct
    17401 actcgtcggg tgcggtgctc gcactggagg cggccgccgc gggcgttccg atcgacaagc
    17461 tcgcgctcta cgagccgccg ttcatcgtcg acgacacgcg cccgcggccg cccgcggacc
    17521 tcccggagca ggtggcggcc ctggtggagg cagaccgccg cggcgacgcc gtcgagctct
    17581 accagaccaa ggccatcggg ctgcccgagg aggtcgtcgt ccagctgcgc aacgcgccct
    17641 tccggcccgg cctcgaggcg atcgcacaca cgctcgtcta cgacgcgatc ctcacccggg
    17701 acctcacgcc cacgaccgag cgcttcggca aggtcaccga gcccacgctc gtgctcggcg
    17761 gaggcgagag ctgggagttc cttcgcaacg gcgcggacgc gctggccgag gtgctgccga
    17821 acgcggagcg gcggacgctg ccgggcgaga gtcacgacat caacccggag gccacggccg
    17881 aggcgctggg ggagttcctc gcctagcgcc cgggccgcac cccgaaccgc ggcaccagcg
    17941 cgggatcgag aaacgcctcg atccccacga tccggccgtc ctcgatctcg agcacgctga
    18001 tcccctgcgg ggtcagcgtg ccgtcctcgc cgcgcagctc ggcggtgacc gcgggccggc
    18061 cgttcgccca ggtcggcctg tgccggatcc gcgtgagatc gccctgcgcg acgatctcca
    18121 ggaagaagcg cgcgatctcg gcgccgccgc tcaaggacgg gttcggcggc atccgcagct
    18181 tcgcgtcctc gtgcagcagc tcgatcagcc cgtcgatgtc gtcgtgctcg aacgcggcca
    18241 cgtagcggtt gagcagctgg tgctcggccg gattcgcctc cgcggacgcc ggctcggtca
    18301 ggtgcgggtc gatcgtgccg cgtgcccgct gtagcgcgct gttgaccgac gcgaccgtcg
    18361 actccagcag ctcggcgacc tccggcgcag tccagccgag cacgtcccgg aagatcagca
    18421 ccgcgcgctg acgtcccggc agctcctgga tcgcacgcag cagcgccagc tccatcccct
    18481 cccggatcgc gtagcgcgcg gcggggtcgt aggtcggcgg agccgtcgcg tccgccggcc
    18541 gatccgggaa tggatcgacc ggctccgggc ggcgcgggcg gcgctcgagc tcgtccaggc
    18601 acgcgttcgt cgcgatccga tagagccacg tctgaaactg cactctgggc tcaaagcgtc
    18661 ccagcgctcg ccacgcccgc aacagcgtct cctgcgtcag gtcctcggca tcctgcgagg
    18721 accccagcat ccggtagcag tgcacctcga gtgcgtgccg gaacggctcg acgagctgac
    18781 ggaacgcccg ctcgtccccg gcctgcgcgg aggccagcag gagacgctga tcggtgtggc
    18841 cccggtcggt caccggctca cgcgccgccg tactcgtcgt ggcggcgcag ccacatctgg
    18901 aactcctcgc cctcgtcgcg gcccctgggc gtccggtcga ggaccgggta gtacgtcatg
    18961 aggaactcga cgccgcgccc gccgctcgag tacgtcaggt agacgtcgtc gccctggcgg
    19021 gcgaaggtgg cgaacgcgaa cccctccgag atgtacgtgc ccacgtcgac gccgcatgcc
    19081 tcggcgttgc gcagggcgat cggtggcatc ccgtcgggaa ccgcctgtag ccccgcctcc
    19141 ctcacctgcg gccgggtcga cccgccgaag tcgaagctga actcgctctc gtagctggac
    19201 gcccagttga agctccaacc catccgctga cggtacgcga ggagcttgtc gagcggcgcc
    19261 ctggagacgc agatcatggt cacgtcgcgg gccgtgaggt gcgggaccag gggatcgaac
    19321 gcgtcggcga tcgatgagtt cgtggggcat ccgtccgtga agtccggacc gaacatgaag
    19381 tggtacatgg agagctgcga gcggccctcg aacagctcga ccagcgtcct cgggccggcc
    19441 tcggtctcga gcgtgtaccc cttctcgacg ggcgtccacg gcagcgcccg gcgctgagcg
    19501 gccagctcgt cctccagccg ggtctgctcc ttctcgcgcg tcagcagctc gtcccgggcc
    19561 gcgtcccact cctcgcgtcc gacgatctcg ttggcggtca tgacggtcat acgttcatct
    19621 ccttttgctt gtagccgacg cccgtgcgtc gtgcgtgggg gtatccgtga gctaggcggc
    19681 gacggcgggg accatcgcgg cgttctggcg ttcgagcgcc ttcgtcacgc cggcgcgcca
    19741 gccgtcggcc agcgactgcg acatcgggtc ggggaagatc tcctcgtcgc cccgctcgac
    19801 gccctccagc gtggcccgcg ccacgtcctc cggcggcgtc ttcgggatgt cgagaccgcg
    19861 caccatgtca gtgtcgatcg ggccggccag gacggcgtga acgctcacgc ccctgccggc
    19921 gagcagtgcg cgcaacgact gggtgaccga cagcgaggcc gccttcgaga ccgaataggc
    19981 ggggagcacc gggaccgccg acaccgcgcc gagcgagacc acgttgacca ccgccccgtg
    20041 gctgcgcacc agtgcgggaa ggaacgcctg ggtgacgtcc agcgtcccgt acaggttcac
    20101 ggccaggtgc tcctcgagcg ccgagcgatc gctcaggtcg tccgggaccg acacgccggc
    20161 gttgttgatc agcacgtcga gcgagtcgac cgcgccggcc gcctcgcgga tctgcgccgc
    20221 gtcggtcacg tccaggatca ggggcgtgac gcgctcgggg gtgacgccca tcggctggcg
    20281 cgtggcggcg tagacccgcg cggcacccct gctgagggct tcgtccacga gcgcgcggcc
    20341 gaggccacga ttggcgcccg ttacgagcac cgtcttgtct gcgattgcca gggacatctc
    20401 cacgtctccg ttgtcgagtt cgggggtcgc accacgttca acggcggcgg gcgccgaaac
    20461 tcatcggtgg agatccggcc ggtcccctca ggccgggtag ggagcggtct cgcgggccgt
    20521 gcgcagggcg tcggcccacc aggcgagctg gtcgagcacg accgtggcgt aggcgggagc
    20581 atcggggtcg agcgggcggc cgtcctccca ggcgctgaag tagttcggga acgcgaggcc
    20641 gtcgcggatg gtcaccgcgt gcagctcggt gagcacgttc tcgaggtgca ggaccgcgtg
    20701 gcggccgccg gccgccccgc cgtagctgac gaaggcgacg ggcttcgcgg tccactgggt
    20761 gaagtgccag tcgatcgccg ccttgaggga ggccgggtag ctgtggttgt actccggcgt
    20821 gacgaggatg aatgcctgcg cctcgtccag cgcggcggtc aggggcgcca tccccggcgg
    20881 gcgcgggtac gactcgccgg cgaacttcgg gggcacggcg ggcagcggat ctccgacgcc
    20941 ggccttggcg aggctgacga cctcgacgtc gaacccgccg tgttcctccg cccgctcggc
    21001 gacccactcg gcgaccgtgt cgccgaaccg cccctcacgc acgcttccta cgacgatgac
    21061 caacctgtgt tctgtgtttg tttcgctcat gcatccaagg tgacgagctt ccgtgccctt
    21121 gaaaagggcc ggggtcaggc tggccccgac agggccaccc tgagcgctga aagtgcgtcg
    21181 cggccccgta gactcgactg atgggcacac ccgcgggcga cttcatccgg gcgaagcgcg
    21241 attcgcttca gcccgagtcg ctggggctgc ccgagcggga gcgccggcgc gcccccggcg
    21301 tccgccgctc cgacctcgcc gcgcgggcgg ggatcagcgt ggagtacctc acccgcatcg
    21361 agcaggggcg ccaccgcaac ccgtcggcct cggtcatcag cgccctcgcg gacgccatga
    21421 gtctcgaccc ggccgagagc gagcacctgc gctacctcgt gaagatcacg ggcggcgcct
    21481 gctccggcca cgagcggccc gagccgccga gccgggaggt gcgccccgac gtgctggcca
    21541 cgctccgcct cctcgagccc gcggtcgcct gcgtgaccaa ccggctcgga gacgtgctcg
    21601 cgtggacgga cgcgttcgag gtcctgatgg ccgaggccgg cctactcgac tccgccgagc
    21661 cgaacctgac gtcatatgtc ttcaccgatc cgcgcgcgag agagttcttc ccggactggg
    21721 accgggtcgc cgacgagcag gtcttcgacc tctggctcgg accgtcggcc gcgacctccg
    21781 agtggttcag gacggagctg gctcccgccg cgggcaagga gttcaccaag cgcttcgacc
    21841 gccacgtgcc cccaccgcgc gcccctctcc acgtccggca ccccggcgcg ggggacctgc
    21901 gctggaaccg ggagaccctc gagctctcgc gcagcgaggc tcagcagatc gtcgtgttcc
    21961 tcccggccga cgacgcgacg acccgcgcca tggaggagct gtcccgccgc tccaggacgg
    22021 gtcgagccct ccgcgcggtg acctagctcg ccgcgggccg cggccgggtc cagcggcgct
    22081 cgtagtcgcc cggcctgggt gcgggtccgg ggagcggctc cctcgcgccg gcacgcagcc
    22141 cggcgagcgc gatctcgacc tggcggcgtc gcgtggcctc ctcggctgga tcgccggacg
    22201 gcgggaaggc gcggctgagg cgctcgatca gctgaaggac gtcgatcgcg ttcgcgtcgg
    22261 ctcgcaggtc gccggcctcc tgcgcgcggc ctgcgagcgc ggtgacgcgg cggcgcaccg
    22321 cgcgtgcgag ccgagtcatc tcggccgtcg cctcgatctc gcccgcgagc ggtccgaagg
    22381 cgccgaccgc gagcccgacg cacgttcgga cgtagtcgca gagcccctcc caggccgtcg
    22441 cggcggcgag ggcgcccgcg gcggccgcga ggttctgctc cagcgacagg aggcagagct
    22501 tctggagcag ctcgtccttg ccgccgtagc ggcggtagag cgtgcccatg ccgacgcccg
    22561 cacgctcggc caccgccgcg atcggcgcgt cggccccgcc ttcggcgaag acggcccggg
    22621 cggcatccag gacgagcgtg tcgttgcgga gcgcctccgc ctggcggcca cggcggggcc
    22681 tgtcgtcgct caccgcgcga agtttatcgg agcgtttcgc tccggatatg tcatgctaac
    22741 ggagtgattc gctccgaaag agaccagggt tgagggcggc ggtcgtcagg gacttcggcg
    22801 gcccggaggt cctgagtgtc gaggcggtgc ccgatcccgc cgccgacccc ggcgagctcg
    22861 tcgtcgaggt cgagtacgcc gggatcacgt tcgtcgagac gcaggttcgg gcgggccgcc
    22921 ccccgaaccc cgcgatgctc ccgcacttgc cgttcgtgcc cggcaacggc gtcgccggtc
    22981 gcgtggccga ggtgggggag ggggccgacc gggggctgct cgggcgcgcg gtcgtgacga
    23041 ccaccggcgg cagcggcggc tacgccgagc gcgtcgccgt gccggccgcg ctgccgatcg
    23101 agatccccga aggggtctcc gctcgggacg cggtggcgct gctcgccgac gggcgcacgg
    23161 cgctcgcgct ggcccgcgtc gccgccgtcg aggccgggga cgtcgtgctc gtggaggccg
    23221 ccgccggcgg ggtggggagc tgcctcgtgc agctcgcgcg cgccgcgggt gcgaccgtgg
    23281 tggccggcgt cggatcggtg cacaaggtgc cgatcgcgcg cgacctcggc gccggcaggg
    23341 tcgtcgacta ctcgcgcgac gggtgggagg acgagatccg cacggagctc ggcgggctgg
    23401 acgtcgtgtt cgacggcgtg ggcggggcga tcggggcatc ggccctgacg ctgctgcggc
    23461 ccggcggccg cctctgccag tacgggatgg cgagcgggag cttcacgaac gtccccgcgg
    23521 agcgccgcga cgtgcaggtg ctgcgcggaa ccgcgctcac gccggccgag tcgcgcgagc
    23581 tgagcatcga ggcgctgcgg ctggccgccg ccggcgagct gtccgcgacc gtggggcagg
    23641 agtacccgct cgagcgggcg gcggcggccc acgccgcgat cgaggcgcgg tcgacgatcg
    23701 gcaagacgct gctgcgcgtc gcgcggatca gctgatcgcg ctcaccatgg aacgggtccg
    23761 gcgctgctca ggaactgccc gctgagagac gcgtcctcgg agagcgcgaa gcgagcgacg
    23821 gtctccgcgg cgtcctccgg ggtgagcggc gcgttgtcgc ggttcatcgg cgtgaggtcc
    23881 gtctgcacga agccggggca gaccgagtag atcgcgatgc cagcgtcctc gagcgccttg
    23941 gagagcgcca cggtgatccc gttcagcgcg gccttcgagg actgataggc agggacgacg
    24001 acggagtagt acggcgactc gggatctccc tggtcggcca gggaccccat cgtcgaggag
    24061 acgttgacga tccgggcgtg ctcggccttg gtgagcagag gcaggaacgc ctcgatggcg
    24121 gcgaccgcgc ccaggacgtt cgtcgcgaag gtctcgcgga acagatcgag gctcaccggg
    24181 ccgttctgct cggcggtc
//
LOCUS       DASVPL010000004         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_235244, whole genome shotgun sequence.
ACCESSION   DASVPL010000004 DASVPL010000000
VERSION     DASVPL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235244"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1575
                     /locus_tag="VF517_00185"
     CDS             220..1575
                     /locus_tag="VF517_00185"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX8051380.1"
                     /translation="MRTGLRIGAGSAWWGDRIEPARLSAERGDLDYLCFETMAEATVS
                     AAQVRKRRDPDFPGYDTYLDDRMRAVLPGCLERGTRIVSNQGWINPEGAAERVAEWLR
                     RLGARGVKVAAVSGSLIGEKVFGFGAAILENGRPVETLRGDMVSAEAYLGAEPIVEAL
                     RQGARVVMTSRVADPSLFLAPLMHEFGWGPLDFARLGPGTGVGHLLECGAQVTGGYFA
                     DPGFKDVPDPWDLGFPIAEVEPDGSTVITKLAGTGGAIDRMTATEQLLYEVHDPANYV
                     TPDVVADFSTARLEEVGPDRVRVSGIGGKPRTPTLKVSIGCQEGFIGEDMFFYAGPGA
                     LRRAQLAKRVLEERFRIVGLQAEEVRVDFLGVNAIHGSASPADGPEPYEVAVRVAART
                     RTREEAAKVGREVDGMAVSGIGMTGKRVPFQDRTREVIGVWSTLIPREAVPWRVTMHE
                     S"
BASE COUNT          229 a          564 c          650 g          212 t
ORIGIN      
        1 tcgaggcgct ccaggggcac gtggcggcca tcctgcggga gccggaggtc aaggaccgcc
       61 tgctcgagct cggcgccacc ccggccggcg acacgccgga cgagttccgc cggcggatcg
      121 cggccgagat cgagaagtgg cgggccgtgg cgcgggcggg caacatccgg atcgagtagc
      181 gcggcggcgg ggccggcggg catcgcggag gaacggacca tgcggacggg gctgcgcatc
      241 ggggctggat cggcgtggtg gggcgatcgc atcgagcccg cgcggctcag cgcggagcgg
      301 ggcgatctcg actacctctg cttcgagacc atggcggagg cgaccgtctc cgccgcgcag
      361 gtccgcaagc ggcgcgatcc ggacttcccc gggtacgaca cctacctcga cgaccggatg
      421 cgggccgtgc tgcccggctg cctcgagcgc ggcacccgga tcgtcagcaa ccagggctgg
      481 atcaacccgg agggcgcggc ggagcgcgtg gcggagtggc tgcgccgcct cggcgcgcgc
      541 ggggtgaagg tcgccgccgt ctccggcagc ctgatcggcg agaaggtgtt cggcttcggc
      601 gccgccatcc tcgagaacgg ccggccggtg gagaccctgc gcggcgacat ggtttcggcc
      661 gaggcctacc tcggcgccga gccgatcgtc gaggcgctgc ggcaaggggc ccgggtggtg
      721 atgacctcgc gcgtcgccga tccctccctg ttcctcgccc cgctgatgca cgagttcggc
      781 tggggcccgc tggacttcgc gcgcctcggc ccgggcaccg gcgtcggcca cctgctggaa
      841 tgcggcgcgc aggtgacggg gggctacttc gccgacccgg gcttcaagga cgtgccggac
      901 ccctgggacc tcggcttccc tatcgccgag gtggagcccg acggcagcac ggtgatcacc
      961 aagctcgccg gcaccggcgg cgcgatcgac cggatgacgg cgaccgagca gctgctctac
     1021 gaggtgcacg accccgcgaa ctacgtcacg cccgacgtcg tggccgactt cagcaccgcg
     1081 cggctcgagg aggtcggccc ggaccgggtg cgggtttccg gcatcggcgg caagccgagg
     1141 accccgacgc tcaaggtctc gatcggctgc caggagggct tcatcggcga ggacatgttc
     1201 ttctacgccg gccccggggc gctgcggagg gcgcagctgg cgaagcgcgt cctggaggag
     1261 cgcttccgca tcgtcggcct gcaggcggag gaggtgcggg tcgacttcct cggcgtcaac
     1321 gccatccatg gctcggcgtc gccggcggac gggcctgagc cgtacgaggt ggccgtgcgc
     1381 gtcgccgccc gcacccgcac gcgcgaggaa gcggcgaagg tgggccgcga ggtggacggg
     1441 atggcggtgt cggggatcgg catgaccggc aagcgggtgc cgttccagga ccgcacgcgc
     1501 gaggtcatcg gcgtatggtc cacgctgatc ccgcgcgagg cggtgccgtg gcgcgtcacg
     1561 atgcacgaga gctgaagggg aggcgcctat gcgggtcagg ctgcacaacg tcgcgcacgt
     1621 gcgctccggc gacaagggcg acaccgcgtg catca
//
LOCUS       DASVPL010000005        22230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_157018, whole genome shotgun sequence.
ACCESSION   DASVPL010000005 DASVPL010000000
VERSION     DASVPL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22230
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157018"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1356
                     /locus_tag="VF517_00190"
     CDS             115..1356
                     /locus_tag="VF517_00190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX8051381.1"
                     /translation="MLTSPTFVIVGAGLAGAKAAETLRAEGFDGRLLLVGQEAERPYE
                     RPPLSKAYLRGEADRDSLYLHADGFYAAHDIELRTSTPVRSIDPAGRRLELASGERIG
                     YDRLLLATGAAPRRLPVPGADLDGVHYLHSGQDADLLAAAATRAEHVVVVGTGWIGSE
                     AAASLRQLGRQVTLLGPDSAPLARVLGPEVAGVYRDLHADHGVRLVLGARVTGFRGHG
                     RVDAVVTEDGRAIECDLVLVGAGAVPRTELAEAAGLPVGNGVLVNEQLEAVGAAGVYA
                     AGDVAAAWHPRYQTYLRVEHWANALNQGPAAARNMLGRSMPYARLPYFYSDQYDLGME
                     YTGFATTWDDVVFRGDTGAREFIAFWLHDGRVVAGMNVNVWDVTEPIQELIRSRSQVD
                     MDALRDPSTPLDQVAREEVAR"
     gene            1669..2016
                     /locus_tag="VF517_00195"
     CDS             1669..2016
                     /locus_tag="VF517_00195"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEX8051382.1"
                     /translation="MAISNAMVKLYKDQFGRGPTRARTHYAGPDTIISTLQDSLTPAE
                     RSLVAMGEHQRLRDVRMFFQYASEDEFRGAVEEITGRKVWAFVSGIDSKQDVSVEVFY
                     LEPEVGSDGGDAA"
     gene            complement(2047..2994)
                     /locus_tag="VF517_00200"
     CDS             complement(2047..2994)
                     /locus_tag="VF517_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX8051383.1"
                     /translation="MAGFPEQQPGLLYLTEGGQETELMYKHGHELPEFAMYPLLDDRR
                     AVADLTDMYTRYLETAAAHGFAALMGGLDYRASPDWASKLGISRDGLAEYQLRSIEFL
                     RDVARPFQGQIPQVLIVGIVGPRGDAYGQNRTITAGEAEEYHRVQLETLRQAGVDLVS
                     AMTFNNVPEGVGVARAAARAGLPLSLSFMVDSSGRLLTGASLKDAVEAVDREAGDARP
                     DFYGINCSHPVEFEPALVAGPWLERIRSLRPNAAMAEKQALCRIGHLEAGDPEALGAH
                     MGGLARRLPRIDVWGGCCGTWDDHLRAIAQQLRPLESAV"
     gene            complement(3043..3342)
                     /locus_tag="VF517_00205"
     CDS             complement(3043..3342)
                     /locus_tag="VF517_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX8051384.1"
                     /translation="MNNSDLVVMTKASAKPGKEEEVLRALRDVAEAGRAQSGCVDYRI
                     LRAADDSTATINFERWSSEDARDAFLAGPEVERFSAAVSGAFAESPQPVSYEAID"
     gene            complement(3361..3837)
                     /locus_tag="VF517_00210"
     CDS             complement(3361..3837)
                     /locus_tag="VF517_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051385.1"
                     /translation="MHDQDYTTTFSVDETPEEVFDAIANVRGWWSEDIEGDTDRLGSV
                     FFYNFKDVHRGTFKITEYAHGRRIVWHVLQNYFNFVADSTEWTGTDIVFEVSRNGERT
                     DFRFTHVGLKPSEECYDVCHDAWKFYVETSLRDLISTGQGEPNKKDTLSENHPVPK"
     gene            complement(3883..4590)
                     /locus_tag="VF517_00215"
     CDS             complement(3883..4590)
                     /locus_tag="VF517_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051386.1"
                     /translation="MATIAGKSVLVTGSNRGIGRALVEEALRRGAGRVYAATREPFAP
                     SDERVTNLVFDVTDTAQIGRAVADVESLDILINNAGAVQYDDLSDRSAIEQQLAVNFF
                     GPYEVTQALLPLLTQARGAIVNNLSLNALAPMPLIPAYSISKAAAFSLTQSLRALLAG
                     KGVSVHAVITGPTDTDMNRGIDIPKAAPELVAAAILDGVERDEEEIFPDPMSSDLGES
                     WQAGATKALERSLAALA"
     gene            complement(4721..5443)
                     /locus_tag="VF517_00220"
     CDS             complement(4721..5443)
                     /locus_tag="VF517_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051387.1"
                     /translation="MQFTSKVRLDDGVLERDFTLGEIPGVLWTPGSAPAPLILIGHTG
                     GFHKRLPRLVARARLYAGEYGFAVAAIDAPGHGDRPRSAADEQARADLRRAAREGEPA
                     DEIVDAFILPLVEGAVPEWRTTLDALLSLPEIDGPVGYDGMMAIGIRLAAVEPRISAA
                     AFFAMSFVPGALREEARQLTIPLQVLLQWDDEGMERQPVLDLFDAFGAKEKTLHANLG
                     GHTGVPWFEVEAAARFFARHLS"
     gene            5676..6116
                     /locus_tag="VF517_00225"
     CDS             5676..6116
                     /locus_tag="VF517_00225"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8051388.1"
                     /translation="MAWTTEHTEYTSASPQRIWELWSDVSTWPLWDEGLERVTLDGPF
                     AAGTKGRLKPAGGPAVGLELTDVRPGAGFSDVTRLPLARMRFEHSAVRDGERTRVTHR
                     VTITGPGTPLFSRLIGRGIANDLPQTIGTLARMAAERDAVPTPG"
     gene            6113..6553
                     /locus_tag="VF517_00230"
     CDS             6113..6553
                     /locus_tag="VF517_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX8051389.1"
                     /translation="MSGGSHAPGDSPGFLLWRVTNRWQRTIVAELGPLDLTHVQFVLL
                     ASVWWLARNGEAPPTQRDVAEHARTDPMMTSQVLRVLERRGLVTRARDPDDRRARRLS
                     PTARGTRLAERAIAVVEAADRAFFEAAEPDVVLRVLRLLDGPGR"
     gene            complement(6578..7144)
                     /locus_tag="VF517_00235"
     CDS             complement(6578..7144)
                     /locus_tag="VF517_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8051390.1"
                     /translation="MGKLIYSMGVSADGFIAGPDGDFSWTEPDEELFRFHTDRVRQLG
                     AHLCGRRLYETMLYWETADQDPALGTAEREFAAIWQRLPKVVFSTTLENVEGNARLAR
                     DSVAEEVRRVEEQVDGDLEVGGAALAASCIELDLVDDYRLFVHPVIVGGGTPFFPPTD
                     ASLELELVETRSFPSRVVYLRYQRARQE"
     gene            complement(7149..7661)
                     /locus_tag="VF517_00240"
     CDS             complement(7149..7661)
                     /locus_tag="VF517_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8051391.1"
                     /translation="MATRPRGEPRSGCPINAAVEVIGDHWSLLVLRDIMFGNRRHFRV
                     LQESSEEGIASNILADRLQRLVSAGLLTRTDAGRGRRVTYSLTEAAIGLVPVMAVLGE
                     WGLRNRATTPTLRVRAELLAEGGPALWADFMADLRTEHLGAPPPPGGGPTATERLAAA
                     YAEAVARQED"
     gene            7754..8359
                     /locus_tag="VF517_00245"
     CDS             7754..8359
                     /locus_tag="VF517_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8051392.1"
                     /translation="MSDTTCHMSISLDGFVAGPDQSRDNPLGKGGREVHLWHLGHERA
                     NDANETAAGWLLRPRGAYVMGRNMFGPIRGEWDEDWDGWWGPEPPYHAPVFVLTHHAR
                     ESIEMEGGTSFHFVTEGFDAAYAQAREVAGEDGVDIAGGASTVRQALAAGVIDELTLD
                     VAPVLLGSGERLFDGVESFDFEPVEVLHSPLATHIRYRRAE"
     gene            complement(8734..9435)
                     /locus_tag="VF517_00250"
     CDS             complement(8734..9435)
                     /locus_tag="VF517_00250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8051393.1"
                     /translation="MTYHDLHDDFDAGLAARFHEEVLAATFSRDELVSPESLAENLRA
                     ATALVTIALDEQGSVLGGIVGDWFAEPHVLLISYLAVRPGLRGQAIGTELRARAVATW
                     PEQRPIHLVVAEVHDPRRWQSEEGEASLARLRLFERFGGQLLDVPFVQPALEPGTERV
                     RGFLLLVFHVDDRIRAGGGEAVRAGPIATWIRQYYAMTEGAEPPYDPELAALLARVEA
                     SDSVRLMPLTAVTAA"
     gene            complement(9432..11561)
                     /locus_tag="VF517_00255"
     CDS             complement(9432..11561)
                     /locus_tag="VF517_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051394.1"
                     /translation="MNAAVPWDAIDHEWHERRFDLLHDTVERFGGLDVERLDELFTPG
                     SQAEPEPVLADSFGAVDGSTEARGLLATYVYGKCLERWEKSEDHGPIEKVLEALRGVL
                     GRNGHDDPVLALVVEQTHCMEHAMGVENALMCSCPVAMEQQAAAVVEGTGRVLNGRRR
                     PAGNTQLVALLRGDAATQRIYYSAVRDTAAAVLAMLEAEAPGPAKLGKAIRELRAAER
                     HQSVRGDVYESELRAHRVGLAALEDAATLPRVWVDDADVVYLYPFAFEDQTEESLDAL
                     LARLDESWSPELGPKRIRPLSVERPLLTNVWEQPGAAEPMYRVVEIRLPDVTVIPTAG
                     EMWKFDCQVALRLSRIGNHCLRVGSQLEDTDVHDLNQALRRGSRSMGNEELISGELRW
                     DKFVDYAGEMIAGLAGALGVATVGEPSADFHVALDAHRLSIQRGRRRRRLPFERPRKS
                     SRPATAAELEGAVGASLLFHPVRHLASSLEEWVRYPPPEVRNLMQGAGYTDDLVVRTA
                     NTTVLYMPSSPDWSCSAYAELVEFVASVPPLLASWEAEVLEREERLRLDLEGASPLTL
                     EQVLDREKQLRALESNVRHELAKLHSRRLVRDRVHREFLDRLWMAAGLESLEDEIERE
                     LAVMATLLERLSTRAATIAEETRRKTRRKVEIILGVIAVISVTGFFSWVNDNFGLDAR
                     WVAGGESALLLVLIVAVVVLVSREDAT"
     gene            complement(11612..11821)
                     /locus_tag="VF517_00260"
     CDS             complement(11612..11821)
                     /locus_tag="VF517_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051395.1"
                     /translation="MALRALVAVLLVLASVAALPAFPSPVAAGGPRFRKLCKPGRLNV
                     AVGRSNDAARGATPGRVTVVVRGPR"
     gene            complement(12075..12148)
                     /locus_tag="VF517_00265"
     tRNA            complement(12075..12148)
                     /locus_tag="VF517_00265"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12112..12114),aa:Met,seq:cat)
     gene            12220..12702
                     /locus_tag="VF517_00270"
     CDS             12220..12702
                     /locus_tag="VF517_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051396.1"
                     /translation="MADTFLRIYMNDQLAAGLVFRELARRSQRSNAGTELGDALERVA
                     SAIAEDIETFERMMERLHLPRNRVKSVLALGGERAGRLKLNGRVRSYSPLSRFVELDA
                     LAIGIDGKKLLWSNLRDHANLAKRLPDVDFDHLIERAQWQRDQLEPFRAKAGSESLGA
                     "
     gene            complement(12723..13310)
                     /locus_tag="VF517_00275"
     CDS             complement(12723..13310)
                     /locus_tag="VF517_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263657636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(13149..13151),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX8051397.1"
                     /translation="MFGTSSERQTARAPGDAGELADMELENHAGEVRRLGDLWEDGPA
                     VLVFLRHYGUIHCRGHAVQLHRDREKFDAAGARLVLIGQGTPAHAQHFREEQNVEGLE
                     ILVDPDRAAYEVAGAKVATMSELFGVKSMVAGIKRATGDRVMQGAVKGHPAQLGGVLV
                     VAKGGKIAWAHLADNAGDNPPNDDVLAAVERAQAA"
     gene            13336..14253
                     /locus_tag="VF517_00280"
     CDS             13336..14253
                     /locus_tag="VF517_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX8051398.1"
                     /translation="MRVAVVDFGTNSTRLLVADVAGGRLTELDRRTNVTRLGQGVDAT
                     GRLADEAIERVLATVAGYRTAIDDLGAERIVAVATSAVRDSSNGDEFRARLRDGFDID
                     ARTISGDQEARLTFLGATSGRPAGDETLVIDIGGGSTELVVGRAGAEPEFHVSTQAGS
                     VRQTERHLPSEPVPDSELRALRDEVRGIVADAVPEDVRRAVEAGIAVAGTATSLAAID
                     QRLDPYDPDRVHGYSLTLAAAERILATLAGLPLERRRAVPGLHPDRAPTIVAGAAILV
                     EAMRAFALDAVEVSEADILHGAALSAAAE"
     gene            complement(14512..16167)
                     /locus_tag="VF517_00285"
     CDS             complement(14512..16167)
                     /locus_tag="VF517_00285"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HEX8051399.1"
                     /translation="MSVETQAASEQAALHGGRLVAKRLRAAGVSKLFTLSGGHLFSIF
                     DGCREEGIDLVDVRHEQSAAFAAEGWAKATREPGVAALTAGPGVTNGMSALASAQMNS
                     SPIVVLGGRAPAMRWGMGSLQEIDHVPFVAPVTKLARTAEPTAAISDMVDEALRAATE
                     PPTGPTFLDFPLDHVFMEGPISGAGPVELHDPLAAPAAEGVEAAAELLRRAERPVVMA
                     GTGLYWARGEHALLRLCEELGVPVFLNGLARGCVPADNPLFFSRARGAALKGADVALV
                     VGVPMDFRLGFGGSFGDETQIVVVGSSPAPRPQPRPVAAELYGGVAATLDALRSAGAG
                     GPDRTAWVTQLQGVEDEKRAAEQAELTDERAPLHPMRVYGELNQVLDRGTIVIGDGGD
                     FVSYAGRMIETYEPGCWMDPGPYGCLGSGPGYAIAAKVARPDRPVCLLLGDGAFGFSG
                     LEFDTMVRHGLPVVGVMGNNGIWGLEHHPMKFLYGYSVAAHLQPELRYDRVVEELGGH
                     GELVQRPEELRPALERAFASGKPALVNVLTDPEVAYPRKANLA"
     gene            complement(16179..17744)
                     /locus_tag="VF517_00290"
     CDS             complement(16179..17744)
                     /locus_tag="VF517_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8051400.1"
                     /translation="MLVLFARWAYRRLGSRYLYAYIAFEFVSALMVTLGTVGIFSLYQ
                     EMTGGELRRIVLVSGTCVVVGLVSGTVKLRRDLAPLQAWMSGTRGEHGANAAWRAAVS
                     APREVVMSEAWRPVVFVVAPVSVYITLELGLPPYSVLVCGAGTMVAVAYTAILHFFAS
                     ELALRPVLEEICEHLPPGFATEGLGVPLRWKLLGALPLINVITGVIVSGLSSDGQAHL
                     NELGVDVLVAVLVAFTVSFELTVLLTRSITVPIGALLDATERVRSGDLSARVPVVSGD
                     EMGQLARSFNAMMSGLEERERLRAAFGSYVAPAVVERVLEEGELLEGDEVEVTVLFVD
                     IQDFTAFAERASARETVSYLNDFFGLAVPILTDHGGHANKFVGDGILGVFGAPERMRD
                     HADRAVRAACEIAHATSERYGDALRIGVGINSGPVSAGTVGGGGRLEFTVIGDPVNVA
                     ARVERATRQTGDTVLLTEATRCLLSDPRIEVEERGELPFKGKSDPVALYTPRVDAERP
                     APVGEPQPARAGR"
     gene            complement(17773..19278)
                     /locus_tag="VF517_00295"
     CDS             complement(17773..19278)
                     /locus_tag="VF517_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8051401.1"
                     /translation="MPKETPSAGRMAAMAVFSLTCFGLLLFLWLSFGGPIPLKPKPYE
                     LKVAFPEATTLAEQADVRIAGVSVGKVQKKELDKGGSRTKVTLNIEQKYAPIPNDTRA
                     ILRQKTLLGETYVELTPGRSGAPKLADGGQLKIGQVEPTVELDEILRIFSPETRDSFR
                     DWIRSGARQIEGRSQDLNFALANLAGFAQDGAGVLKVLDDQRGATQRLVRNTGVVFNA
                     LTERNGQLRQLIKNSESTFAVTSAQQDNLARVFEIFPTFLSESRLTLARLQTFARNTS
                     PLVRDLKPVADDLGPTVRDLSFLGPDLERLFRRLEPNIDAAPKNLPQAERFLRGASPL
                     FEALHGFLPELNPIISFANFEQDPLAHFFVNGTAALKYQLAPGANPGVPRYLLGFAGA
                     INGKSLSLQQVRPQSDRGNAYIAPNNYRRGVRFGIPESFDCRPDHPDGDGRTGGSVGR
                     RDPVDEGGVEVPPCFVQPPFLWDGNYFPRLERGKAPLVKAPFGLEGTTRTP"
     gene            complement(19278..20645)
                     /locus_tag="VF517_00300"
     CDS             complement(19278..20645)
                     /locus_tag="VF517_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8051402.1"
                     /translation="MSVRIIRKHLRDFVAVGILILISLAVAAYIISQQEARPYFPVIE
                     SSPYEIKVEFSDAQAVIPGQGQSVRVAGVQVGKIGKVEVKNGVAVVSMNLERKKIENG
                     ELKVLSDATAQLRPRTGLKDMFIELDPGTDGEELKEKDVLPVQNSEPDVDIDEVLAAL
                     DSDTRSYLQLLITGLGKGLAGRGKDLNATFKALAPTQRDLRRVTEAIAGRKRELKSLI
                     HNYGDLTNTLADRDNDVRRLVTASNATLSAFAQENDNLAAAVSLLPGTLRQTERTLSK
                     VNTYAGVLQPTLQSLRQPFRQLATANRAVLPFVREAEPIVRKEIRPFVQVARPYVNTL
                     RPAARNLRIAAPDLSDALFELNRFFNMASFNPKGREALPQSQSAARARDEGYLFWIGW
                     VSQNTVSLFSTADAQGPFRRAYFGTTCDLVREEVARTPAAGLLLGFNELLAPTGICGG
                     GGTNP"
     gene            complement(20642..>22230)
                     /locus_tag="VF517_00305"
     CDS             complement(20642..>22230)
                     /locus_tag="VF517_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8051403.1"
                     /translation="TFQSANNLQPKSPVRIAGVDVGKVTKVEPLPGGTGAARVTMEIQ
                     KKGLPLHKDAQLKIRPRIFLEGNFFVDLQPGTPSAPLLNEEGSVPVQQTATPVQFGEV
                     LTALQRDTRSDLQTFFKEYAREGLGNGGAQAYNRALSDSPEALRNVAIANEASLGQRP
                     HDLSRLLRGQQRLLSQLGSDPEALKDLVTNLNLTAEALGRNDEALRAAVPALRDTLRV
                     GQPALVSLNNALPTLRVFAREALPGVQTSGPTIRASMPFIRQARLLVRREELRGLASD
                     LRRAIPDLARVDRATIPLLDNQRALSACTSNVLVPFARTPIPAPELEDKFPDSSNQPF
                     YKQGPRSFVGLSGESRITDANTPFFHIQFSSGPANLLVYNEGGEQAFAAVSSPPEGVR
                     PARPDKRPGFRPDVPCETQESPDMAAAGGTPDAAFSPQGELLPGGVIPPSLVPPLPKR
                     TAAAPKAQQLQLQWIQESMRRQAAGQATPDPLAFSTKTYPRELRRAGLATTPKGKIYK
                     RGDAKAKARALASEQQESVR"
BASE COUNT         3212 a         7987 c         7808 g         3223 t
ORIGIN      
        1 cgtcacctcc gccctgcacg gcctgctccg cagcgaccag cgctcgcggc aggagttctt
       61 cgcccttgct ggcacggccg cctaactacg acgacccaca aaggggaagt tccgatgctc
      121 accagtccga cgttcgtgat cgtcggtgcc ggcctggccg gtgccaaggc ggccgagacc
      181 ctgcgcgccg aaggcttcga cggccgcctc ctgctggtcg gccaggaggc cgagcggccg
      241 tatgagcgcc cgcccctgtc caaggcctac ctgcgcggcg aggccgaccg cgacagcctc
      301 tacctgcacg ccgacggctt ctacgccgcc cacgacatcg agctgcgcac ctcgaccccg
      361 gtccgctcga tcgacccggc cggccgccgg ctggagctgg cctcgggcga acggatcggc
      421 tacgaccggc tgctgctggc caccggggcc gccccgcgcc gcctccctgt gcccggggcg
      481 gacctggacg gcgtccacta cctgcacagc ggccaggatg ccgacctcct cgccgccgcg
      541 gccacccgcg ccgagcatgt cgtggtggtc gggaccggct ggatcggcag cgaggccgcc
      601 gcctccctgc gccagctcgg ccggcaggtc accctcctcg gcccggacag cgccccgctg
      661 gcccgggtgc tgggccccga ggtcgccggc gtctaccgcg acctgcacgc cgaccacggc
      721 gtccggctgg tcctcggggc gcgggtgacc ggcttccgcg gccacggccg ggtcgacgcg
      781 gtcgtcaccg aggacggccg cgccatcgaa tgcgacctgg tgctggtggg cgccggcgcc
      841 gtcccacgca ccgagctggc cgaggcggcc ggcctgccgg tcgggaacgg ggtcctggtc
      901 aacgagcagc tggaagcggt cggagcggcc ggtgtctacg ccgccggcga cgtggccgcc
      961 gcctggcacc cccgctacca gacctacctg cgggtcgagc actgggccaa cgcgctcaac
     1021 cagggcccgg ccgcggcccg caacatgctc ggccgctcga tgccgtatgc gcggctgccc
     1081 tacttctact ccgaccagta cgacctcggc atggagtaca ccggcttcgc cacgacgtgg
     1141 gacgacgtgg tcttccgagg cgacaccggc gcgcgcgagt tcatcgcctt ctggctgcac
     1201 gacgggcgcg tggtcgccgg catgaacgtg aacgtctggg acgtgaccga gccgatccag
     1261 gagctgatcc gctcgcggag ccaggtcgac atggacgcgc tccgcgaccc gagcacgccg
     1321 ctcgaccagg tcgcgcgcga ggaggtcgca cgatgaacgg ggtccaggcg ctcaccgagc
     1381 cggtgacgac cctcggtcgc gggccctgaa ctcgactatc ctctgaccaa gaggttcccg
     1441 gactccccga gcgacaggca agcgctcgac ggccgcttgc cttgtgcacc agcgactcgg
     1501 gactcttgcc ggttcatcga accgggcaag aggacccagc cggccagaag ccgcgcccgg
     1561 tccgtacccc ctcctccccg cgcggttctt gcctagaggg acttgccgct ggaggatcca
     1621 atggacacgc aggacgcacc cgatcgacag cagggccaat cagtctccat ggccatctcg
     1681 aacgcgatgg tcaagctcta caaggaccag ttcggccgcg ggccgacccg ggcccggacg
     1741 cactacgccg ggcccgacac gatcatcagc acgctgcagg actcactcac ccccgcggag
     1801 cgcagtctcg tggcgatggg cgagcaccag cgactgcggg acgtgcgcat gttcttccag
     1861 tacgcgtcgg aggacgagtt ccgaggcgcc gtcgaggaga tcaccggccg caaggtctgg
     1921 gcattcgtga gcggaatcga cagcaagcag gacgtctcgg tcgaggtctt ctacctggag
     1981 cccgaggtcg ggagcgacgg cggggacgcg gcgtagcgcg tcgcccgcgc cagggcagtc
     2041 cgccgatcag accgcgcttt cgagcggacg cagttgctgt gcgatggcgc ggaggtggtc
     2101 gtcccaggtc ccgcagcatc cgccccagac gtcgatgcgc ggaaggcgcc gagcgagccc
     2161 gcccatgtgc gcgccgagcg cttcgggatc gcccgcctcg agatgtccga tccggcatag
     2221 cgcctgcttc tcggccatcg ccgcgttcgg ccgcaggctc cgaatccgct cgagccacgg
     2281 gcccgcgacc agcgccggct cgaactcgac gggatgcgag cagttgatgc cgtagaagtc
     2341 cggccgggca tcgcccgcct cccggtccac cgcctccacc gcgtccttca gcgaagcgcc
     2401 tgtgagcagc cggccgctcg agtcgaccat gaacgagagc gagagcggca gaccggccct
     2461 cgccgcggcc ctcgcgacgc cgacgccctc ggggacgttg ttgaacgtca tcgcgctcac
     2521 gagatcgacg ccggcctgcc gcagcgtctc gagctggacc cggtggtact cctcggcctc
     2581 gccggcggtg atcgtgcgat tctggccata ggcgtcgccg cgtgggccaa caatgccgac
     2641 gatgaggacc tgcgggatct gcccctggaa cggccgggcg acatcacgca ggaactcgat
     2701 cgagcgcagc tgatactcgg cgagcccgtc gcgtgagatc ccgagcttcg acgcccagtc
     2761 cgggctggct cggtagtcca ggccgcccat cagcgcggcg aagccatgcg ccgcggcggt
     2821 ctccaggtac cgcgtgtaca tgtcggtgag gtccgccacg gcccgccgat cgtcgagcag
     2881 cgggtacatc gcgaactcgg gcagctcgtg cccgtgcttg tacatgagct ccgtctcctg
     2941 accgccctcg gtcaggtaga gcaggcccgg ttgctgttcc gggaatccag ccatgacggg
     3001 tttatatggc acgccgcggc gcgcggcttc gagggccgtg ggtcagtcga tcgcctcgta
     3061 cgacaccggt tgcggcgatt cggcgaaggc accggagacg gccgcactga acctctccac
     3121 ctccggaccg gcgaggaacg cgtcacgtgc gtcttcggac gaccaccgct cgaagttgat
     3181 cgtcgcggtg gagtcatccg cggcgcgcag gatgcggtag tcgacgcagc ccgactgcgc
     3241 acgaccggcc tccgcgacat cgcgcagcgc tcggagaacc tcctcctcct tgcccggctt
     3301 cgcgctggcc ttcgtcatca cgacgagatc tgagttgttc atggtcgttg gtcctttctg
     3361 ttacttgggg acggggtggt tctccgacag cgtgtccttc ttgttgggct cgccctggcc
     3421 cgtggagatc aggtcacgca gactcgtctc gacgtagaac ttccacgcgt cgtggcagac
     3481 gtcgtagcac tcctcggacg gcttcaggcc gacgtgggtg aagcggaagt cggtcctttc
     3541 accattcctc gacacctcga agacgatgtc ggtgcccgtc cactcggtgc tgtcggcgac
     3601 gaagttgaag tagttctgca gcacgtgcca gacgatcctc cggccgtgcg cgtactccgt
     3661 gatcttgaac gtgccgcgat ggacgtcctt gaagttgtag aagaacacgg agccgagcct
     3721 gtccgtgtct ccctcgatgt cctccgacca ccatccgcgc acgttggcga tggcgtcgaa
     3781 gacctcctcc ggcgtctcgt ccaccgagaa ggtggtcgtg taatcctggt cgtgcatcgg
     3841 cttgcctttc gtcgtgactg tgggttgatg gggacttgcg ctctaggcca gggccgccag
     3901 cgagcgctcg agcgccttgg tggcgccggc ctgccagctc tcgccgaggt cgctcgacat
     3961 cgggtcgggg aagatctcct cctcgtcgcg ctcgacgccg tcgaggatgg ccgccgcgac
     4021 gagctccgga gcggccttcg ggatgtcgat cccgcgattc atgtcggtgt cggtgggccc
     4081 ggttatgacg gcatgcacgc tcacgccctt gcccgccagg agcgcgcgca gcgactgcgt
     4141 cagggagaac gcggccgcct tcgagatcga gtaggccggg atgagcggca tcggcgcgag
     4201 ggcgttcagc gacaggttgt tcacgatggc gccccgggcc tgcgtgagca gtggcagcag
     4261 ggcctgcgtc acctcgtacg gcccgaagaa gttgacggcg agctgctgct cgatcgccga
     4321 gcgatcgctc aggtcgtcgt actgcaccgc ccccgcgttg ttgatgagga tgtcgagcga
     4381 ttcgacgtcg gcgacggccc gcccgatctg cgccgtgtcg gtgacgtcga agaccaggtt
     4441 cgtcacccgc tcgtccgacg gcgcgaacgg ctcgcgcgtg gcggcgtaga cccgccccgc
     4501 acctcgcctg agggcctcct ccaccagcgc ccgtccgatg ccgcggttgc tccccgtgac
     4561 cagtaccgac ttgcctgcga tggtcgccac gcgacctcct tgcccgttgt cgtgatgccg
     4621 tgtcgttgat ggagatacgg gcaggcgccg agatccatcg gtccgaccgc gcccgccgcc
     4681 caccgcccgc tggcggcgtg acggcggcgc cggccggcag ctagctcaga tgtcgggcga
     4741 agaaccgcgc cgcggcttcc acctcgaacc acgggacgcc ggtgtgcccg cccagattgg
     4801 cgtgcagcgt cttctccttg gcgccgaagg cgtcgaagag gtccaggacc ggctgccgtt
     4861 ccatcccctc gtcgtcccac tgcagcagga cctgcagcgg aatggtcaac tgccgggcct
     4921 cctcccgcag agcgccgggc acgaagctca tggcgaagaa agcggcggcc gagatgcgcg
     4981 gctcgaccgc cgccagtcgg atgccgatgg ccatcatccc gtcgtacccg accggcccgt
     5041 cgatctcggg cagggaaagg agggcgtcca gggtggtccg ccattccggc accgcccctt
     5101 cgaccagcgg gaggatgaac gcgtcgacga tctcgtcggc cggctcgccc tcccgcgccg
     5161 cccggcggag gtcggcgcgg gcctgctcgt cggcggccga acggggccgg tcgccgtgcc
     5221 cgggggcgtc gatggcggcc accgcgaagc cgtactcccc cgcgtagagc cgggcccggg
     5281 ccaccagccg cgggagccgc ttgtggaagc cgccggtgtg gccgatcagg atcagcgggg
     5341 ccggtgcgga tccaggcgtc cacaggacac cggggatctc gccgagggtg aaatcgcgct
     5401 cgaggacgcc gtcgtcgaga cgcactttgg aggtgaattg caaggtcgtg cctttcggga
     5461 gtgcttagac ggcgctcccg gacgacctag cgtccgaccg tgaccgcgga ggggagcacc
     5521 cagatcgata ctgcgttttt cacgggtgcc acctcctctt ctttctgtcg cacggcctgc
     5581 aggaaggcta ccgcggccgc cccttgacgc gggagcacaa gcggccagcg cgtattaata
     5641 tgtgcgcata ctatttcccc cggagaggac ggctcatggc ttggacgacg gagcacaccg
     5701 aatacacctc ggcttcgcca cagaggatct gggagctgtg gagcgacgtc tccacctggc
     5761 cgctgtggga cgagggcctc gagcgcgtga cgctcgacgg cccgttcgcg gcgggcacga
     5821 aggggcggct gaagccggcg ggcgggccgg ccgttggcct cgagctgacg gatgtacgcc
     5881 ccggcgccgg attctccgac gtcacgaggc ttcccctggc gcgcatgcgc ttcgagcaca
     5941 gcgcggtccg cgacggtgag cgcacccggg tgacgcaccg cgtcacgatc acggggccgg
     6001 gcacgccgct cttctcgcgg ttgatcggcc gtggcatcgc aaacgacctc ccgcagacga
     6061 tcgggacact cgcccgcatg gccgcggaac gcgacgccgt cccgacgccg gggtgagcgg
     6121 cggaagccac gctccggggg acagcccggg gttcctcctc tggcgggtca cgaaccgctg
     6181 gcagcggacc atcgtcgcgg agctgggccc actagacctg acgcacgtgc agttcgtgct
     6241 gctcgcgtcg gtctggtggc tggcgcgcaa cggcgaggcc ccgccgactc agcgcgacgt
     6301 cgccgagcac gcgcggaccg acccgatgat gacctcccag gtcctgcggg tactcgagcg
     6361 ccggggcctc gtcacgcgcg cccgcgaccc ggacgaccgg cgcgcgcgcc ggctgtcgcc
     6421 cacggcccgc gggacacggc tcgcggagcg cgcgatcgcg gtggtcgagg cggccgaccg
     6481 cgccttcttc gaggccgcgg agccggacgt ggtgctccgg gtgctcaggt tgctggacgg
     6541 ccccggtcgc tgaggggcgg ggcgccgtcc cgcaggctca ttcctgccgg gcccgctggt
     6601 agcgcaggta gacgacccgg gaggggaacg agcgcgtttc gacgagctcg agctcgaggc
     6661 tcgcatccgt gggtgggaag aacggcgtgc cgcctcccac gatcactggg tggacgaaga
     6721 ggcggtagtc gtccacgaga tcgagctcga tgcagctggc cgcgagcgcg gcaccgccga
     6781 cctcgaggtc gccgtcgacc tgctcctcga cacggcggac ctcctcggcg acgctgtcgc
     6841 gggcaagcct cgcgttccct tccacgttct cgagggtcgt cgagaagacc accttcggaa
     6901 gccgctgcca gatcgccgca aattcgcgct cggccgtccc gagcgcgggg tcttggtcgg
     6961 cggtctccca gtagagcatg gtctcgtaca ggcggcggcc gcagagatgc gcgccgagct
     7021 ggcgtacacg atcggtgtgg aagcggaaga gctcctcgtc cggctcggtc cagctgaaat
     7081 caccgtccgg accggcgatg aatccgtccg ccgagactcc catggagtag atcagcttgc
     7141 ccaccgcctc agtcctcttg gcgggcgacg gcttcggcgt aggccgcggc caggcgctct
     7201 gtcgcagtgg gaccaccgcc cggcggcggg ggtgccccga gatgctcggt acgcaggtcg
     7261 gccatgaagt ccgcccagag cgcggggccg ccctcggcca gtagttcggc tcgcacgcgg
     7321 agcgtggggg tggtggcccg gttgcgcagg ccccactcgc cgagcacggc catcacgggg
     7381 accagcccga tcgccgcctc ggtgaggctg taggtcacgc ggcgtccgcg accggcgtcc
     7441 gtgcgcgtga gcagcccggc ggagacgagc ctttgcaggc ggtcggcgag gatgttcgag
     7501 gcgatgccct cctccgagga ctcctgcaaa acgcggaaat gccgccggtt tccgaacatg
     7561 atgtcccgca ggacgagcag tgaccagtgg tcgccgatca cctccaccgc cgcgttgatc
     7621 gggcagcccg agcgcggttc gccgcgggga cgcgtcgcca tcgacgtgga tggtagttgc
     7681 agtttgccac caccctccgg ataaatacgg tacggtgccg cgcttgcaaa ccgcaatcac
     7741 ttggaggacg ccagtgtcgg acaccacctg ccacatgtcg atctcactcg acggtttcgt
     7801 cgccggaccg gaccagagcc gggacaaccc actggggaag ggtggcaggg aggtccacct
     7861 ctggcatctc ggccacgagc gcgcgaacga cgccaacgag acggcggcgg gctggctcct
     7921 gcgcccccgc ggggcctacg tcatgggtcg caacatgttc gggccgatcc gcggcgagtg
     7981 ggacgaggac tgggatggat ggtggggccc cgagccgccc taccacgcgc cggtcttcgt
     8041 gctcacccac cacgcccgtg agtcgatcga gatggagggc ggcacgagct tccacttcgt
     8101 caccgagggc ttcgacgccg cctacgcgca ggcacgcgag gtggccggcg aggatggggt
     8161 cgacatcgcg ggcggcgcat cgaccgtccg tcaggcgctc gccgccgggg tgatcgacga
     8221 gctcacgctc gacgtggccc ccgtcctgct cgggtccggc gagcggctgt tcgacggagt
     8281 cgagtccttc gacttcgagc cggtcgaggt actgcactcc ccgctggcca cgcacatccg
     8341 ctaccggcgg gcggagtgag gcgccgagct tcgtgagtag cgggggcggg gtttggacgc
     8401 ctgtccgcga cggcgtggcg agtcgttgag acacgcgtcc tcggatagct gccggaggcg
     8461 tacgtcggca ccgggcggta gacctcgtgc atggccagcc ccgtcagcga gttgctagca
     8521 accgaggccg ccgtcgcgaa gctcggtgcg cgtcaggctg gtcaaccttc cccctgtccc
     8581 gctactccgt agcgctcaac tgaagcctcc caaagaacga cgaaccggtc aaccgggagg
     8641 aatgagaggt tgcccacacg aacggagaag aacttcgtgc gggcgtggat ccgcgctggc
     8701 gctgttcgcg atgacggccg agcccgggcg gcgctaggcg gcggtcaccg cggtcagcgg
     8761 catcagccgc accgagtccg aggcctcgac gcgcgcgagc agtgctgcga gctccgggtc
     8821 gtaggggggc tctgcgccct cggtcatcgc gtagtactgc ctgatccagg tcgcgatcgg
     8881 cccggcgcgg accgcctcgc cgccgccggc ccggatccgg tcgtcgacgt ggaagaccag
     8941 cagcaggaag ccgcgcaccc gttcggtgcc gggctccagc gcgggctgga cgaacgggac
     9001 gtccagcagc tggccgccga agcgctcaaa aagtcgcagc cgggcgagtg aggcctcgcc
     9061 ctcctcgctc tgccagcgcc gcgggtcgtg gacctccgcc accaccaggt ggatcgggcg
     9121 ctgctcgggc caggtggcga ccgcacgcgc ccgcagctcg gtcccgatcg cctggccccg
     9181 caggcccggc cgcacggcga ggtacgagat cagaagcaca tgcggctcgg cgaaccagtc
     9241 gccgacgatg ccgccgagca cgctcccctg ctcgtccagc gcaatcgtga cgagcgcggt
     9301 ggccgcgcgc aggttctcgg ccaggctctc gggcgagacc agctcgtcgc gcgagaaggt
     9361 cgccgccagg acctcctcat ggaagcgcgc cgccaggcca gcgtcgaagt cgtcgtgcag
     9421 gtcgtggtag gtcacgtggc gtcctcgcgg gagacgagga cgacgacggc cacgatcagc
     9481 acgaggagca acgccgactc gccccccgcg acccagcggg cgtcgagccc gaagttgtcg
     9541 ttgacccagc tgaagaagcc ggtcacggag atgacggcga tcaccccaag gatgatctcc
     9601 accttccggc gggtcttccg gcgggtctcc tctgcgatcg tcgcggcgcg cgtggagagc
     9661 cgctcgagca gcgtggccat caccgccagc tcccgttcga tctcgtcctc gagcgactcg
     9721 agcccggccg ccatccagag cctgtccagg aactcgcggt gcacgcggtc ccgcacgagc
     9781 cggcgcgagt ggagcttggc cagctcgtgg cggacgttgc tctcgagcgc gcgcagctgc
     9841 ttctcgcgat cgagcacctg ctcgagcgtc agcggcgagg cgccctcgag atccagcctc
     9901 agtcgctcct cgcgctcgag cacctcggcc tcccacgagg cgagcagcgg cggcacggag
     9961 gcgacgaact cgaccagctc ggcgtaggcc gagcagctcc agtcgggcga cgacggcatg
    10021 tagaggacgg tcgtgttggc ggtgcgcacg acgaggtcgt ccgtgtagcc cgccccctgc
    10081 atcaggttcc ggacctcggg gggcggatag cggacccact cctcgagcga gctggcgagg
    10141 tgtcgcaccg ggtggaacag gagcgaggcg ccgacggccc cctcgagctc cgcggcggta
    10201 gcgggtcgcg acgacttccg cggccgctcg aacggcagcc gccgccggcg ccggccgcgc
    10261 tggatcgaga gccggtgggc gtcgagcgcc acgtggaagt cggcgctcgg ctccccgacc
    10321 gtggcgacgc cgagcgcgcc ggccagcccg gcgatcatct cgcccgcgta gtccacgaac
    10381 ttgtcccagc gcaactcgcc gctgatcagc tcctcgttgc ccatgctccg cgagccgcgg
    10441 cgcagggcct ggttgaggtc gtggacatcg gtgtcctcga gttgcgagcc gacgcgcagg
    10501 cagtggttgc cgatgcggct cagccgcagc gccacctggc agtcgaactt ccacatttcg
    10561 ccggcggtcg ggatgaccgt cacgtccggc aggcggatct ccacgacgcg gtacatcggt
    10621 tcggccgcgc caggctgctc ccagacgttg gtgagcagcg gccgctccac cgagaggggc
    10681 ctgatgcgct tcgggccgag ctcggggctc caactctcgt cgaggcgggc cagcagggcg
    10741 tcgaggctct cctcggtctg gtcctcgaac gcgaacgggt agaggtacac gacgtcggcg
    10801 tcgtcgaccc agacccgcgg cagcgtggcg gcgtcctcga gcgccgcgag cccgacacga
    10861 tgcgcccgca gctcgctctc gtacacgtcg ccgcgaaccg actggtgccg ctcggccgcg
    10921 cgcagctcgc ggatcgcctt gccgagcttc gccgggccgg gggcctcggc ctcaagcatc
    10981 gcgaggacgg cggcggccgt gtcgcggacg gcgctgtaat agatccgctg ggtcgcggcg
    11041 tccccgcgca gcagcgcgac gagctgcgtg ttcccggccg gacggcgccg gccgttcagg
    11101 accctcccgg tcccttcgac gacagcggcg gcctgctgct ccatggccac cgggcacgag
    11161 cacatgaggg cgttctcgac gcccatcgca tgctccatgc agtgcgtctg ctccaccacg
    11221 agggcgagga ccggatcgtc gtggccattg cggccgagca cgccgcggag cgcctcgagc
    11281 accttctcga tcgggccatg gtcctcggac ttctcccagc gttcgaggca cttgccgtag
    11341 acgtaggtgg cgaggagccc ccgggcctcg gtgctcccat ccaccgcgcc gaacgagtcc
    11401 gccagcacgg gctcgggctc ggcttgactg cccggcgtga agagctcgtc gagccgctcg
    11461 acgtcgaggc cgccgaagcg ctcgaccgtg tcgtggagca ggtcgaacct gcgctcgtgc
    11521 cactcgtggt cgatggcgtc ccacgggact gccgcgttca cacgccgacc atctcagtcg
    11581 agtgctggcg cgtcaacccg cccctggggg gtcagcgcgg accgcgaacg accacggtca
    11641 cgcggcccgg cgtcgcgccg cgcgcggcgt cgttcgagcg gccgacggcg acgttcagac
    11701 gccccggctt gcacagcttg cgaaaccgcg gtccacctgc tgcgacaggg ctgggaaagg
    11761 caggaagcgc cgcgaccgac gccaggacga gcaggacggc gacgagggca cggagagcca
    11821 tgcatcaccc tatgtggcac tccggatttg gcgcaagtcc ccaatccggg ggcatgaaca
    11881 ggtgccgggc tcggggccgg cttagcgaaa tcgccgcgcg tgtcgctgag gggctctggg
    11941 ccgctccgtc gcgcgagctc gggtcccgaa tctgcaagtt ccaaaacaaa ttggaacctt
    12001 tttgaaacct tgagtccgat gcgtccggcc cctaaaaaag cacgaagggc ccgcttttgc
    12061 aggcccttcg taagtagcgg gggcgggatt cgaacccgcg accttcgggt tatgagcccg
    12121 acgagctacc tggctgctcc accccgcgtc gcttcgacct aggttagcgc cgcgccaccc
    12181 ccggttcaag cacccgcgcc ggggtagccc cgtccccgaa tggccgacac gttcctgcgc
    12241 atctacatga acgaccagct cgccgcgggc ctcgtctttc gcgagctcgc ccgccgctcg
    12301 cagcgcagca acgcgggcac agagctcggc gacgcgctcg agcgggtcgc gagcgccatc
    12361 gccgaggaca tcgagacctt cgagcggatg atggagcgcc tccacctccc acgcaaccgc
    12421 gtcaagtccg tgctggcctt gggcggcgag cgcgccggcc ggctcaagct caacggccgc
    12481 gttcgctcct actcgccgct cagccgcttc gtcgagctcg acgccctggc gatcgggatc
    12541 gacgggaaga agctgctgtg gtcgaacctg cgcgaccacg cgaacctcgc gaagcgcctg
    12601 cccgacgtcg acttcgacca cctgatcgag cgcgcccagt ggcagcgcga ccagctcgag
    12661 ccgttccgcg cgaaggccgg ctcggagtcg cttggcgcct agcgccgggc ccggctaagc
    12721 ggctaagcgg cctgcgcgcg ctcgacggcc gcgagcacgt cgtcgttcgg cggattgtcg
    12781 ccggcgttgt ccgccaggtg cgcccaggcg atcttgccgc ctttcgccac cacgagcacg
    12841 ccaccgagct gcgcgggatg acccttgacc gccccctgca tcacgcggtc cccggtggcc
    12901 cgcttgatcc cggccaccat ggacttgacc ccgaacagct cgctcatcgt cgcgaccttc
    12961 gccccggcga cctcgtaggc cgcgcgatcg ggatcgacca ggatctcgag tccctcgacg
    13021 ttctgctcct cgcggaagtg ctgcgcgtgc gccggcgtgc cctgaccgat cagcacgagg
    13081 cgcgcgccag cggcgtcgaa cttctcgcgg tcgcggtgca actgcaccgc gtgtcctcgg
    13141 cagtgaattc agccgtagtg gcggaggaag accagcacag ccggcccgtc ctcccacagg
    13201 tcgccgagcc ggcgcacctc gcccgcgtgg ttctccagct ccatgtccgc cagctcgccc
    13261 gcgtcgccgg gtgcccgcgc cgtctggcgc tcgctggatg tgccgaacat gcccacagat
    13321 gaaaggctac cggccatgcg cgtcgccgtg gtcgactttg gcacgaactc cacccggctc
    13381 ctcgtcgccg acgtggccgg cggccgcctg accgagctcg accgccgcac caacgtgacg
    13441 cgcctcgggc agggcgtcga cgccaccggc cggctcgccg acgaggcgat cgagcgcgtc
    13501 cttgcgaccg tggccggcta ccgcaccgcc atcgacgacc tcggcgccga gcggatcgtc
    13561 gcggtcgcca ccagcgccgt ccgcgacagc tccaacggcg acgagttccg cgcccggctg
    13621 cgcgacggct tcgacatcga cgcccgcacg atctccggtg accaggaggc gcggctcacc
    13681 ttcctcggcg ccacgtccgg acgcccggcc ggcgacgaga cgctcgtgat cgacatcggc
    13741 ggcgggagca ccgagctggt cgtcggccgc gccggcgccg agccggagtt ccacgtctcg
    13801 acccaggcgg gctcggtccg ccagaccgag cgccacctcc cgagcgagcc agtgcccgac
    13861 tccgagctgc gcgcgctgcg cgacgaggtg cgcgggatcg tcgcggacgc ggtgcccgag
    13921 gacgtgcggc gcgcggtcga ggcgggcatc gcggtcgcgg gcaccgcgac ctcgcttgcc
    13981 gcgatcgacc agcgcctcga cccgtacgac cccgaccggg tgcacggcta ctcgctcacc
    14041 ctcgcggcgg ccgagcggat cctcgcgacg ctcgccgggc tgccgctcga gcgccgccgc
    14101 gcggtgcccg gcctccatcc ggaccgcgcg cccacgatcg tggcgggcgc ggccatcctc
    14161 gtcgaggcca tgcgcgcctt cgccctggac gcggtggagg tcagcgaggc cgacatcctg
    14221 cacggagccg cgctgtcggc cgcggcggaa taaatacggg gccggtcgca gtcgttgcga
    14281 ccggccccgc ttgaagcggc cccatcagga cgggcagggg tacgtgcctt gaaggggcct
    14341 gagcggtccg gacggcggga ggcgccggtt tgatgcggct tcgcccgaac ctatgtgccg
    14401 agactgccca ccagcaccta ttggcacaag tcggcccacc ccgacagcgc cagtataaac
    14461 cgtgtccccc tcctggggca agcacgaatg agcgtaaaag cgcctacgcc ctcaggccag
    14521 gttcgccttg cgcgggtagg ccacctcggg atcggtcagc acgttgacca gagcgggctt
    14581 acccgaggcg aacgcgcgct cgagcgcggg gcgcagctcc tccggtcgct ggaccagctc
    14641 cccgtgcccg ccgagttctt cgaccacgcg gtcgtagcgc agctccggct gcaggtgagc
    14701 cgccaccgag tacccgtaga ggaacttcat cggatggtgc tcgagccccc agatcccgtt
    14761 gttgcccatc acgccaacga ccggcagccc gtgccgtacc atcgtgtcga actcgagccc
    14821 ggagaagccg aacgcgccgt cgccgaggag caggcagacc gggcggtcgg gacgggcgac
    14881 cttcgcggcg atcgcgtagc cggggcccga gccgaggcag ccgtaagggc ccgggtccat
    14941 ccagcagccc ggctcgtagg tctcgatcat gcggcctgcg tacgagacga agtcgccgcc
    15001 gtcgccgatc acgatcgtcc cgcggtcgag cacctggttc agctccccgt acacgcgcat
    15061 tgggtgaagc ggtgcgcgct cgtcggtcag ctcggcctgc tcggccgcgc gcttctcgtc
    15121 ctcgacgccc tgcagctgcg tgacccacgc cgtccggtcc ggtccgcccg cgcccgcgga
    15181 ccgcaacgcg tcgagcgtcg cggccacgcc gccgtatagc tccgcggcca ccgggcgcgg
    15241 ctgcgggcgc ggcgccggcg acgagcccac gaccacgatc tgcgtctcgt ccccgaacga
    15301 gccgccgaac ccgagccgga agtccatcgg gacaccgacc accagcgcca cgtccgcgcc
    15361 cttgagcgcc gccccccgcg cgcgagagaa gaacagcggg ttgtcggcgg gcacgcagcc
    15421 gcgcgcgagc ccgttcagga acacgggcac cccgagctcc tcgcagagac gcagcagcgc
    15481 gtgctcgccg cgcgcccagt acagcccggt gccggccatc acgaccggtc gctcggcacg
    15541 gcgcagcagc tcggcggcgg cctcgacgcc ctcggcggcc ggcgcggcca gcgggtcgtg
    15601 cagctccacc ggcccggcac cggagatcgg gccctccatg aagacgtggt cgagcgggaa
    15661 gtcgaggaag gtcggcccgg tgggcggctc ggtggccgcg cgcagcgcct cgtcgaccat
    15721 gtccgagatc gcggccgtcg gctcggcggt gcgcgcgagc ttcgtgaccg gcgcgacgaa
    15781 cggcacgtgg tcgatctcct ggagcgaccc catcccccac cgcatcgccg gcgcccgccc
    15841 gcccagcacc acgatcggcg acgagttcat ctgcgcggac gcgagcgcgc tcatcccgtt
    15901 ggtcacgccc gggccggccg tcaaagcggc gacccccggc tcgcgcgtgg ccttagccca
    15961 cccctccgcg gcgaacgctg cggactgctc gtggcgcacg tcgacgaggt cgatcccctc
    16021 ctcgcggcag ccgtcgaaga tcgagaagag gtggccgccg gagagcgtga agagcttgct
    16081 gaccccggcc gcgcgcagcc gcttggccac cagccggccc ccgtgaagag ccgcctgctc
    16141 cgacgccgcc tgcgtctcga ccgacatcgc ggcgcagcct accggcccgc gcgcgccggc
    16201 tgcggctcgc cgaccggcgc aggccgctcg gcgtcgaccc gcggtgtgta gagggccacc
    16261 gggtcggact tgcccttgaa cggcagctcg ccgcgctcct cgacctcgat ccgcgggtcg
    16321 ctcagcaggc agcgcgtggc ctcggtgagc agcacggtgt caccggtctg gcgcgtggcg
    16381 cgctcgaccc gcgcggcgac gttgaccgga tcgccgatca ccgtgaactc gagcctcccg
    16441 ccgccgccca ccgtgcccgc cgacaccggc ccggagttga tcccgacgcc gatccgcagc
    16501 gcgtccccgt agcgctcgga cgtggcgtgc gcgatctcgc aggccgcgcg caccgcgcga
    16561 tcggcgtggt cgcgcatgcg ctccggcgca ccgaacaccc cgaggatccc gtcgccgacg
    16621 aacttgttcg cgtgcccgcc gtggtcggtc aggatcggca cggccagccc gaagaagtcg
    16681 ttcaggtagg agaccgtctc gcgcgcgctc gcgcgctcgg caaacgcggt gaagtcctgg
    16741 atgtcgacga acaggaccgt cacctcgacc tcgtcgccct cgagcagctc gccctcctcg
    16801 agcacgcgct cgaccacggc gggggcaacg tagctgccga acgccgcacg cagccgctcg
    16861 cgctcctcga gcccactcat catcgcgttg aagctgcgcg cgagctggcc catctcgtcg
    16921 cccgagacga ccggcacccg cgccgacagg tcacccgagc gcacccgctc cgtggcgtcg
    16981 aggagggcgc cgatcgggac cgtaatcgag cgcgtcagga gcacggtcag ctcgaacgag
    17041 acggtgaacg cgaccagcac cgcgacgagc acgtccacgc cgagctcgtt gaggtgcgcc
    17101 tggccgtcgc tcgagaggcc actcacgatc acccccgtga tcacgttgat cagcggcagc
    17161 gcccccagca gcttccagcg cagcggcacc ccgagtccct cggtggcgaa gccgggcggc
    17221 aggtgctcgc agatctcctc gagcaccggc cgcagcgcga gctccgacgc gaagaagtgc
    17281 aggatcgccg tgtaggcgac cgcgaccatc gtgcccgcgc cgcacacgag caccgagtac
    17341 ggcggcagcc ccagctcgag cgtgatgtac acggagaccg gcgcgacaac gaacacgacc
    17401 ggtcgccacg cctcgctcat caccacctcg cggggcgccg acaccgccgc ccgccacgcc
    17461 gcgttcgcgc cgtgctcccc gcgcgtgccg ctcatccacg cctggagcgg cgccaggtcg
    17521 cgccgcagct tcaccgtgcc cgagaccagt ccgaccacga cgcaggtccc cgacacgagc
    17581 acgatccgcc ggagctcgcc gcccgtcatc tcctggtaga gcgagaagat cccgacggtg
    17641 ccgagcgtca ccatcagagc ggagacgaac tcgaaggcta tgtaggcgta caggtaccgg
    17701 gagcccagcc gccggtacgc ccagcgggcg aacaggacga gcatcggccc ccgattgtgc
    17761 cgtaccggct ggctacgggg tccgcgtggt gccttcgagc ccgaacggcg ccttcacgag
    17821 cggggccttg ccgcgctcga gccgcgggaa gtagttgccg tcccacagga acggcggctg
    17881 gacgaagcac ggcggcactt ccacgccgcc ctcgtcgacg ggatccctgc gcccgaccga
    17941 cccaccggta cgcccgtcgc cgtccggatg gtccggcctg cagtcgaagc tctcggggat
    18001 gccgaaccgg acgccgcggc ggtagttgtt cggcgcgatg taggcgttgc cgcggtcgga
    18061 ctggggccgc acctgctgga gcgacaggct cttgccgttg atcgcgccgg cgaagccgag
    18121 caggtagcgc ggcaccccgg ggttggcgcc cggcgcgagc tggtacttga gcgcggcggt
    18181 gccgttcacg aagaagtggg cgagcgggtc ctgctcgaag ttcgcgaacg agatgatcgg
    18241 gttgagctcc ggcaggaacc cgtgcagcgc ctcgaacaga gggctcgcgc cgcgcaggaa
    18301 gcgctcggcc tggggcaggt tcttcggcgc cgcgtcgatg ttcggctcga ggcggcggaa
    18361 caggcgctcg aggtccggcc cgaggaagct gaggtcgcgc acggtcgggc cgaggtcgtc
    18421 ggcgaccggc ttgaggtcgc gcacgagcgg gctggtgttg cgcgcgaagg tctggagccg
    18481 cgcgagcgtg agccgcgact cggacaggaa cgtcgggaag atctcgaaga cccgagcgag
    18541 gttgtcctgc tgggcggacg tgaccgcgaa cgtgctctcg gagttcttga tgagctggcg
    18601 gagctggccg ttgcgctcgg tcagcgcgtt gaacaccacg ccggtgttcc tcacgagccg
    18661 ctgggtcgcg ccgcgctggt cgtccagcac cttgagcacg cccgcgccgt cctgggcgaa
    18721 gcccgcgagg ttggcgagcg cgaagttgag gtcctggctg cgaccctcga tctgccgcgc
    18781 cccggaacgg atccagtcgc ggaacgagtc gcgcgtctcc gggctgaaga tgcggaggat
    18841 ctcgtcgagc tcgacggtcg gctcgacctg cccgatcttc agctggccgc cgtccgccag
    18901 cttcggtgcg ccgctcctgc ccggcgtcag ctccacatag gtctcgccga gcagcgtctt
    18961 ctgccgcagg atcgcgcgcg tgtcgttcgg gatcggcgcg tacttctgct cgatgttcag
    19021 cgtgaccttc gtgcggctgc cgcccttgtc gagctccttc ttctggacct tgccgaccga
    19081 cacgcccgcg atccggacgt ccgcctgctc ggccagggtc gtggcctcgg ggaaggcgac
    19141 cttcagctcg tacggcttcg gcttcagcgg gatcggcccg ccaaacgaga gccacaggaa
    19201 gagcagcagg ccgaagcacg tcagcgagaa gacggccatc gcggccatcc ggccggcgct
    19261 gggggtttcc ttgggcatta ggggttcgtc ccccctccgc cgcagatccc ggtcggcgcg
    19321 agcagctcgt tgaagccgag cagcagtccg gcggccggcg tgcgcgcgac ctcctcgcga
    19381 accaggtcgc aggtggtgcc gaagtacgcc cggcggaacg ggccctgagc gtcagcggtc
    19441 gagaagaggc tgacggtgtt ctgagacacc cagccgatcc agaacaggta gccctcgtcg
    19501 cgagcgcggg cggcgctctg gctctgcggc agcgcctcgc ggcccttcgg gttgaaggag
    19561 gccatgttga agaagcggtt cagctcgaac agcgcgtctg agaggtccgg cgcggcgatc
    19621 cgcaggttgc gggccgccgg gcgcagcgtg ttcacgtacg ggcgcgcgac ctgcacgaac
    19681 ggccggatct ccttgcgcac gatcggctcg gcctcgcgca cgaacgggag caccgcgcgg
    19741 ttcgcggtcg cgagctggcg gaacggctgg cgcagcgact gaagcgtggg ctggagcacg
    19801 ccggcgtagg tgttgacctt cgacagcgtg cgctcggtct ggcgcagcgt ccccggcagc
    19861 aggctcaccg cggcggcgag gttgtcgttc tcctgcgcga acgccgacag cgtcgcgttc
    19921 gaggcggtca cgagccggcg cacgtcgttg tcgcgatcgg ccagcgtgtt ggtgaggtcg
    19981 ccgtagttgt ggatgaggct cttgagctcc cgcttgcgcc cggcgatcgc ctcggtcacg
    20041 cgccgcaggt cgcgctgcgt cggcgcaagc gccttgaagg tcgcgttgag gtccttgccg
    20101 cggcccgcga ggcccttgcc gaggcccgtg atcaggagct ggaggtacga gcgggtatcg
    20161 gagtcgagcg ccgcgagcac ctcgtcgatg tccacgtccg gctcgctgtt ctgcaccggg
    20221 aggacgtcct tctccttcag ctcctcgccg tcggtgcccg ggtcgagctc gatgaacatg
    20281 tccttcagac ccgtgcgtgg gcggagctgc gcggtcgcgt cggagagcac cttcagctcg
    20341 ccgttctcga tcttcttgcg ctcgaggttc atgctcacga cggcgacgcc gttcttgacc
    20401 tcgaccttgc cgatcttgcc cacctggacg ccggcgacgc gcacgctctg gccctggccc
    20461 gggatcacgg cctgggcgtc cgagaactcg accttgatct cgtacgggct cgactcgatc
    20521 acggggaagt agggccgcgc ctcctgctgg gagatgatgt aggcggccac cgcgagcgag
    20581 atcaggatca ggatcccgac cgcgacgaag tcgcgcaggt gcttgcggat gatgcgcacg
    20641 ctcatcgcac cgactcctgc tgctcggagg ccagtgcgcg cgccttcgcc ttcgcgtcgc
    20701 cgcgcttgta gatcttgccc ttcggcgtgg tcgccaggcc ggcgcggcgc agctcacgcg
    20761 ggtaggtctt cgtggagaag gcgagcgggt cgggtgtcgc ctggccggcc gcctggcggc
    20821 gcatcgactc ctggatccac tggagctgga gctgctgcgc cttcggcgcc gcggcggtgc
    20881 gcttcggcag cggcggcacc aggctcggcg ggatcacgcc gcccgggagc agctcgccct
    20941 gcggggagaa ggccgcgtcc ggtgtgccgc cggccgcggc catgtccggg ctttcctggg
    21001 tctcacacgg gacgtcgggc cggaagccgg gacgcttgtc cgggcgggcg ggccgcacgc
    21061 cctccggcgg gctcgacacc gcggcgaatg cctgctcgcc accctcgttg tagacgagca
    21121 ggttggccgg cccgctcgag aactggatgt ggaagaaggg cgtgttcgcg tcggtgatgc
    21181 ggctctcgcc ggagagcccg acgaagctgc gcgggccctg cttgtagaag ggctggttgc
    21241 tcgagtcggg gaacttgtcc tcgagctccg gcgcggggat cggggtgcgc gcgaacggca
    21301 cgaggacgtt gctggtgcaa gcgctgagcg cgcgctggtt gtcgagcagc gggatcgtgg
    21361 cccggtccac gcgcgcgagg tcggggatgg cgcggcgaag gtcggacgcc agcccgcgca
    21421 gctcctcgcg gcgcacgagc aggcgcgcct ggcggatgaa cggcatcgac gcgcggatcg
    21481 tcgggccgga ggtctgcacg cccggcagcg cctcgcgggc gaacacgcgc agcgtcggca
    21541 gcgcgttgtt gagcgacacg agcgccggct ggccgacgcg cagcgtgtcg cgcagcgccg
    21601 ggaccgccgc gcgcagcgcc tcgtcgttgc ggccgagcgc ctccgccgtc aggttcaggt
    21661 tggtcacgag atccttcagc gcctccgggt ccgacccgag ctggctgagc agccgctgct
    21721 ggccgcgcag cagccgcgag aggtcgtggg ggcgctggcc gagcgacgcc tcgttggcga
    21781 tcgcgacgtt ccgcagcgcc tccggcgaat cggagagcgc gcggttgtat gcctgagcac
    21841 cgccgttgcc gagcccctcg cgggcgtatt ccttgaagaa cgtctggagg tcgctgcggg
    21901 tatcgcgctg cagggcggtc aggacctcgc cgaactgcac cggcgtggcg gtctgctgca
    21961 ccgggacgct gccctcttcg ttcagcagcg gcgcactcgg cgtgcccggc tggaggtcga
    22021 cgaagaagtt gccctcgagg aagatgcgcg ggcggatctt gagctgcgcg tccttgtgca
    22081 gcggcaggcc cttcttctgg atctccatcg tgacgcgcgc ggcgccggtc ccgcctggca
    22141 gcggctcgac cttggtgacc ttcccgacgt ccacgcccgc gatgcgcacg ggcgacttcg
    22201 gctggaggtt gttcgcgctc tggaacgtcg
//
LOCUS       DASVPL010000006         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_235610, whole genome shotgun sequence.
ACCESSION   DASVPL010000006 DASVPL010000000
VERSION     DASVPL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235610"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1892)
                     /gene="serA"
                     /locus_tag="VF517_00310"
     CDS             complement(282..1892)
                     /gene="serA"
                     /locus_tag="VF517_00310"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX8051404.1"
                     /translation="MNDRARVLVKEKIGDSGVELLRESGFDVELGVDWDGDELERRIG
                     EFDGILIRSATKLDADLLARATRLRAVGRAGVGVDNVDVEAATKRGIVVANAPQSNVI
                     TAAEHTMALLLALARNVPQAHASLKAGKWERSSFSGVELYEKTLGIVGFGRIGQLVAQ
                     RARGFGMRVVAFDLFVGEERFRELGAERAATSDELYAAADFITIHLPKTPDTENWLDA
                     SAFAKMKDGVRVLNVARGPLVVDDDLRAAIDSGKVAGAALDVFREEPVTDHPLFGYPN
                     VIVTPHLGASTAEATDRAGYQAAEQVVAALAGGVVTSAVNMPAVRAEDLEELGPFVTL
                     ADELGRIGAALAAGASIDAVEVEYLGRIAERDTRLLTVQVLKGILRGHVEEHVNDVNA
                     PAIAEERGIAVAETNRPSARDFSDLVRVTVTSGDASARVVGTTLGRRYRPHLLEAWGS
                     RFNVQLEPHLAVFRYVDQPGMLGRVGTALGKAGVNVISAAVGLRPDAEPGSGEAVMVV
                     TAHSPVPQAVVDALVASNGFVEGRTVSL"
     gene            complement(1889..>2263)
                     /locus_tag="VF517_00315"
     CDS             complement(1889..>2263)
                     /locus_tag="VF517_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051405.1"
                     /translation="EENADLDFVMFLGDYIYESVNDPRFQSTTPDRAIKLPDGIDGAK
                     VPKLMRDRFGVTIAGGQGKLKGQIARIAHCGYFGAFDILTTFSAFEMALRELGHDVEL
                     GSGVGAAQAVFLEAGVPAAASA"
BASE COUNT          295 a          829 c          802 g          337 t
ORIGIN      
        1 gacgccgagg cgggcgagct gctgcgcgat ctcgagcttc tcggcgcggt tcagcgagat
       61 cccgggcgac tgctcgccat cgcgcagggt cgtgtcgaag atccgtaccc ggtcctcttc
      121 cctgcgcgct gtcgtcgtgt ccgtcatgtc gttcctttca gtgctccgct tccgtctcct
      181 ctggctggtc ttgcggctac gcggccgcca cgcgctattc cccccgaggg gggaggagga
      241 gaagtcgaag cgagagctgg aacgtcatct gcgggtccac tctacaggct cacggtgcgg
      301 ccctcgacga acccgttgct cgcgacgagc gcgtcgacga cggcctgggg caccggcgag
      361 tgcgcggtga cgaccatcac ggcctcgccc gacccgggct cggcgtccgg gcgcagcccg
      421 accgccgcgg agatcacgtt gacgccggcc ttgccgagcg ccgtgcccac ccgcccgagc
      481 atgccgggct ggtcgacgta gcggaagacc gcgaggtgcg gctcgagctg gacgttgaag
      541 cgcgagcccc acgcctcgag caggtgcggc cggtagcggc ggccgagggt ggtgcccacc
      601 acgcgcgcgg acgcgtcgcc gctggtgacg gtcacgcgga cgaggtcgct gaagtcgcgc
      661 gcgctcgggc ggttggtctc ggccacggcg atcccgcgct cctccgcgat cgcgggcgcg
      721 ttcacgtcgt tcacgtgctc ctcgacgtgc ccgcgcagga tgcccttgag cacctggacg
      781 gtgagcaggc gggtgtcgcg ctcggcgatc cggcccaggt actcgacctc cacggcgtcg
      841 atcgacgcgc cggcggccag cgccgcgccg atgcgcccga gctcgtccgc gagcgtcacg
      901 aacggcccga gctcctccag gtcctcggcg cgcaccgcgg gcatgttcac cgcgctcgtg
      961 acgaccccgc cggcgagcgc cgccaccacc tgctcggcgg cctgatagcc ggcgcggtcg
     1021 gtggcctcgg cggtggacgc gcccaggtgc ggcgtgacga tcacgttcgg gtagccgaag
     1081 agcgggtggt cggtgaccgg ctcctcgcgg aagacgtcca gcgcggcccc ggcgaccttg
     1141 cccgagtcga tcgccgcccg caggtcgtcg tcgaccacga gcggcccgcg cgcgacgttg
     1201 agcacccgga cgccgtcctt catcttcgcg aacgcgctgg cgtcgagcca gttctccgtg
     1261 tcgggggtct tgggcaggtg gatcgtgatg aagtccgcgg cggcgtagag ctcgtcggac
     1321 gtcgcggcgc gctccgcgcc cagctcccgg aagcgctcct cccccacgaa caggtcgaag
     1381 gcgaccaccc gcatgccgaa gccgcgcgcg cgctgggcca cgagctgccc gatgcgcccg
     1441 aagcccacga tcccgagcgt cttctcgtac agctcgacgc cgctgaagct cgaccgctcc
     1501 cacttgcccg ccttgagcga cgcgtgcgcc tgcggcacgt tgcgcgcgag cgcgagcagc
     1561 agcgccatcg tgtgctcggc cgcggtgatg acgttcgact gcggggcgtt ggcgacgacg
     1621 atgccgcgct tggtggccgc ttcgacgtcg acgttgtcga cgcccacccc ggcgcgcccg
     1681 accgcgcgca gccgcgtggc gcgcgcgagc aggtcggcgt ccagcttggt ggcggaccgg
     1741 atcaggatcc cgtcgaactc gccgatcctg cgctcgagct cgtcgccgtc ccagtcgacc
     1801 cccagctcca cgtcgaagcc cgactcccgc aggagctcta cgccggagtc cccgatcttc
     1861 tccttgacga ggacgcgcgc gcggtcgttc acgcggacgc ggcggccggc acgccggcct
     1921 ccaggaagac cgcctgggcg gcgccgacgc cgctgccgag ctcgacgtcg tgtcccagct
     1981 cgcggagcgc catctcgaac gccgagaagg tggtgaggat gtcgaacgcg ccgaagtagc
     2041 cgcagtgggc gatccgcgcg atctggccct tgagcttgcc ctggccgccg gcgatcgtga
     2101 cgccgaagcg gtcgcgcatc agcttgggca ccttcgcgcc gtcgatcccg tccggcagct
     2161 tgatcgcgcg atcgggcgtc gtcgactgga agcgcggatc gttgaccgac tcgtagatgt
     2221 agtcgccgag gaacatgacg aagtcgaggt cggcgttctc ctc
//
LOCUS       DASVPL010000007         4409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_196176, whole genome shotgun sequence.
ACCESSION   DASVPL010000007 DASVPL010000000
VERSION     DASVPL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196176"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VF517_00320"
     CDS             complement(<1..787)
                     /locus_tag="VF517_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8051406.1"
                     /translation="MPVEAAAVQAPAAASERPRTRRTRGLTLFVSYSGLMGGSERILL
                     DMAPRLEGGTVAVACPEGPLAERLRDAGVHVFPLRRRRLQLRASMRDRLEKPLRLAGQ
                     AREVGELVAALRPAVTVGWGTRAAMACAAAVHRSDPPSALVFQHNDLLQGPLIARAGR
                     TMASRSDLVVALSATVARDLDPSGRLDDRTVIVPPGVDLDAYAGIGPPPPGPPRALVL
                     GAIVGWKRPRLALEAVALAARRLPELQVTLAGPVIDDDGERLAE"
     gene            complement(802..1161)
                     /locus_tag="VF517_00325"
     CDS             complement(802..1161)
                     /locus_tag="VF517_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX8051407.1"
                     /translation="MLRVLLVTLAAAALVAAFPAAGLAQDAGSNQYQDPLAGEPGGGG
                     GGGNSGGGGGNGNAPSSGTQQNVQSQSGSDTTAAATPARDQLPATGSDTWMLALAGAM
                     LLSGGLGLRRVAGRPSL"
     gene            1283..2485
                     /locus_tag="VF517_00330"
     CDS             1283..2485
                     /locus_tag="VF517_00330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8051408.1"
                     /translation="MPTPNTPDVARVALVHDFLLDLRGAERVFLEMCELWPDAPIYTA
                     VYDEGGTEGRFAHRDVRATFLQKLRPSAGNFRALLPLYPTAIESVDLSDFDLVVSSSS
                     AWAHAVICDEHTVHVDYCHNPFRYAWNDRDRTLAARRDPVSRAVLRSLFHRWRQWDWI
                     AAQRVDGYVANSNTTRRRIRSYFNRESRVVYPPVDTARFRPGPVADHYLVLSELMPHK
                     RIDIAVEAFNRLKLPLVVAGDGPDYRRLRALAGPTIKFAGRVSDAEVARLLESCRGLV
                     VTAVEEFGIAAVEAQAAGRPVFSVKGGGALETVVEGVTGSFWDGGPDALAEAIVAFDA
                     ATVDPQECVRSAQRFDSTVFRRALPQEVERVLRESGERPSAPHRPERVPVRPGLRLSR
                     RGRVRPFV"
     gene            complement(2490..>4409)
                     /locus_tag="VF517_00335"
     CDS             complement(2490..>4409)
                     /locus_tag="VF517_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051409.1"
                     /translation="AVAIRSYTPWIGRVYAFDLGGAALGAGAAVPLLWVFDAPTLLVA
                     LGALGAAAALLFAGSGTDRLVAAAALGVVVFATALAGGTEAYYLKPEGPKPLVEKWTP
                     ISRVTSYGRLNGSPNGLVAYDRVIGEIVPYRRGTPLPDWRRTQEGPQSVGYEATGPGH
                     ALIIGGGGGRDILTALAHRQRVDVIELNRRIREVVDEDMRDFSGAPYSLPGVSTEIGD
                     GRSTLAERDTHYDQIHLGFTDTYSANSAQAFALTENNLYTVEAFEEYFEHLNPGGILN
                     VSRPHRENGNEGARATALAMEGLRRSGVRDPKRNVVVLLGTYTNPFNTFEYATTLAKK
                     TAFTAEELARIRAVAAERSEGIAYAPGGPYIEEWADLARAPSPAAFCAKTRYDLCPPT
                     DNRPFFFNIRRLQDLGNSDTRGSLRVPDPVLVLVVTLSVLLALAALAFVLPLWLVRRE
                     GRPPAGALMFFAAIGVGYLVLEIVLIQRFVLFLGFPTYALSVVLFSLLLFTGLGAHLS
                     TRPGEPRRRLVLTLGVAIALMVAAAFGLQPLLRALIELPFALRVAAALVLLAPFGVLL
                     GMAMPLGLQRIDALHPGAVTWAWAINGIASVVASALAVLVALEWGFVAATLVAAAAYV
                     GALLHAVRGRWPASA"
BASE COUNT          582 a         1628 c         1655 g          544 t
ORIGIN      
        1 cctcggcgag gcgctcgccg tcgtcgtcga tcacgggccc ggcgagggtc acctggagct
       61 cgggcagccg ccgcgcggcg agcgccaccg cctcgagcgc cagccgcggg cgcttccagc
      121 ccacgatggc gccgagcacc agcgcgcgcg gcgggccggg ggggggcggg ccgatgccgg
      181 cgtacgcgtc gaggtcgacc cccggcggga cgatcaccgt gcggtcgtcg aggcgtccgc
      241 tcgggtcgag gtcgcgcgcg accgtggccg acagcgccac gaccaggtcg gaccggctgg
      301 ccatcgtgcg cccggcgcgc gcgatgagcg ggccctggag caggtcgttg tgctggaaga
      361 cgagcgccga gggcgggtcg ctgcggtgga cggcggccgc gcaggccatc gccgcgcgcg
      421 ttccccagcc cacggtgacc gcgggccgca gcgccgccac cagctcgccg acctcgcgcg
      481 cctggccggc gagccgcagc ggcttctcga gccggtcgcg catgctcgcg cgcagctgga
      541 ggcgccggcg gcgcaggggg aagacgtgca ccccagcgtc acggagccgc tcggcgagcg
      601 gcccctccgg acaggcaacc gcgaccgttc cgccctcgag ccgcggcgcc atgtcgagca
      661 ggatccgctc ggagccgccc atgaggccgg agtagctgac gaagagggtc agcccgcgcg
      721 tgcggcgcgt gcgtggccgc tcggaagcgg ctgccggcgc ctggaccgcg gctgcctcga
      781 ccggcacggc gcccggcggg gctagaggct cggccggccg gctacccggc gcagcccgag
      841 gccgccgctc aggagcatgg cgccggcgag cgccagcatc caggtgtccg agccggtggc
      901 gggaagctgg tcgcgcgcgg gcgtggccgc cgcggtggtg tccgagccgg actgcgactg
      961 gacgttctgc tgcgtgcctg aggatggcgc gttgccgttt ccgccgccgc ctccgctgtt
     1021 gccgccgcct ccgccaccgc cgggctcgcc ggcgagcgga tcctggtact ggttgctgcc
     1081 cgcgtcctgc gcgaggcctg cggccgggaa ggccgcgacc agcgccgcag cggcgagcgt
     1141 cacgaggagg actcgtagca cggcgcgtat tggtagcaga ccggcgggac ggcgaccgcc
     1201 gggagtacgc gccagaacac cgctgggcct taacactggg accgtggcgt agcggccggt
     1261 tcggggctta gactggcgct gcatgcctac ccctaacacg cccgacgtcg cccgggttgc
     1321 gctggtccac gactttctcc tggatctgcg cggggccgag cgcgtgttcc tcgagatgtg
     1381 cgagctgtgg ccggacgcgc cgatctacac ggcggtctac gacgagggcg gcaccgaggg
     1441 ccgtttcgcg caccgcgacg tgcgcgcgac cttcctccag aagctgcgcc cgagcgccgg
     1501 caacttccgg gccctgctgc cgctctaccc gaccgccatc gagtcggtcg acctctccga
     1561 cttcgacctg gtcgtctcga gctcgagcgc gtgggcgcac gccgtgatct gcgacgagca
     1621 cacggtccac gtggactact gccacaaccc gttccgctac gcctggaacg accgcgaccg
     1681 caccctggcc gcgcgccgcg acccggtcag ccgcgcggtg ctgcgctcgc tgttccaccg
     1741 ctggcgccag tgggactgga tcgccgccca gcgcgtggac ggctacgtgg ccaactcgaa
     1801 caccacgcgg cggcgcatcc gctcgtactt caaccgcgag tcgcgcgtgg tgtacccgcc
     1861 ggtcgacacg gcgcgcttcc gccccggccc ggtcgccgac cactacctgg tgctctccga
     1921 gctgatgccg cacaagcgca tcgacatcgc ggtcgaggcg ttcaaccggc tgaagctgcc
     1981 gctggtcgtg gcgggcgacg gaccggacta ccgccgcctg cgcgcgctcg ccgggcccac
     2041 catcaagttc gccggccgcg tgagcgacgc cgaggtggcg cgcctgctcg agagctgccg
     2101 gggcctggtc gtcaccgccg tcgaggagtt cgggatcgcc gccgtcgagg cgcaggcagc
     2161 cggccggccg gtcttctcgg tgaagggcgg cggggcgctc gagaccgtcg tcgagggcgt
     2221 gaccggcagc ttctgggacg gcggcccgga cgcgctcgcc gaggcgatcg tggcgttcga
     2281 cgccgcgacc gtcgacccgc aggagtgcgt gcgcagcgcg cagcgcttcg acagcacggt
     2341 cttccgccgc gcgctcccgc aggaggtcga gcgggtgctg cgcgagagcg gcgagcgccc
     2401 gtcggcgccg caccgcccgg agcgcgtgcc ggtgcggccc gggctgcggc tctcgcgccg
     2461 tggccgcgtg cggccgttcg tctgagcctc taggcgctcg ccggccagcg tccgcgcacg
     2521 gcgtggagca gggcccccac gtacgcggcc gccgccacca gcgtcgccgc cacgaagccc
     2581 cactcgagcg ccacgaggac cgccagcgcg ctcgcgacca ccgacgcgat cccgttgatc
     2641 gcccacgccc aggtgaccgc gcctgggtgc agggcgtcga tccgctgcag cccgagcggc
     2701 atcgccatcc cgagcagcac gccgaacggc gcgagcagca ccagcgccgc cgccacccgg
     2761 agcgcgaacg gcagctcgat cagggcgcgc agcagcggtt ggaggccgaa ggccgccgcc
     2821 accatcagcg cgatggcgac gccgagcgtc agcacgagcc ggcggcgcgg ctcgcccggg
     2881 cgggtggaca ggtgggcgcc gagccccgtg aacaggagca gcgagaacag cacgaccgaa
     2941 agcgcgtacg tggggaagcc gaggaacagc acgaagcgct ggatcagcac gatctcgagc
     3001 accaggtagc cgacgccgat cgccgcgaag aacatcagcg ctcccgcggg cggccgtccc
     3061 tcgcgccgga ccagccacag cggcagcacg aacgccagcg ccgcgagcgc cagcagcacg
     3121 ctcagcgtga ccacgagcac cagcacgggg tcgggcaccc tcaggctccc gcgggtgtcg
     3181 ctgttgccga ggtcctggag gcggcggatg ttgaagaaga acgggcggtt gtcggtcggc
     3241 gggcagaggt cgtacctggt cttcgcgcag aacgccgccg gcgacggtgc gcgggcgagg
     3301 tccgcccact cctcgatgta cgggccgccg ggggcgtagg cgatgccctc ggagcgctcg
     3361 gcggccacgg cgcgtatccg cgcgagctcc tccgccgtga acgcggtctt cttcgcgagc
     3421 gtggtcgcgt actcgaacgt gttgaacggg ttcgtgtagg tcccgaggag cacgaccacg
     3481 ttccgcttcg ggtcgcgcac cccggagcgc cgcagcccct ccatggcgag cgcggtggcg
     3541 cgcgctccct cgttgccgtt ctcccggtgc ggccgcgaga cgttcaggat cccgccgggg
     3601 ttcaggtgct cgaagtactc ctcgaacgcc tccacggtgt agaggttgtt ctcggtgagc
     3661 gcgaacgcct gcgccgagtt ggcgctgtag gtgtcggtga agccgaggtg gatctggtcg
     3721 tagtgggtgt cgcgctcggc gagtgtcgag cggccgtccc cgatctccgt gctcacccca
     3781 ggcagcgagt acggcgcgcc ggagaagtcg cgcatgtcct cgtccaccac ctcgcggatg
     3841 cggcggttca gctcgatcac gtccacgcgc tgcctgtggg cgagagcggt gaggatgtcg
     3901 cgcccgcctc cgccgccgat gatcagcgcg tggccggggc cggtcgcctc gtagccgacg
     3961 ctctgcgggc cctcctgcgt gcggcgccag tcgggcagcg gcgtgccgcg ccggtagggg
     4021 acgatctcgc cgatcacccg gtcgtaggcg acgaggccgt tgggcgagcc gttcaggcgc
     4081 ccgtacgagg tgacccggct gatcggcgtc cacttctcga ccagcggctt cggcccctcg
     4141 ggcttcaggt agtacgcctc ggtgccgccg gccagggcgg tggcgaagac caccaccccg
     4201 agcgcagccg cggccacgag ccggtcggtg ccggagccgg cgaacagcag cgcggcggcg
     4261 gcgccgagcg cgccgagcgc cacgagcagc gtgggcgcgt cgaagaccca cagcagcggc
     4321 accgcggcac ccgcgccgag cgccgcgccg ccgaggtcga acgcgtagac gcgcccgatc
     4381 cacggcgtgt acgagcggat cgcgaccgc
//
LOCUS       DASVPL010000008        14489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_157194, whole genome shotgun sequence.
ACCESSION   DASVPL010000008 DASVPL010000000
VERSION     DASVPL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14489
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157194"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VF517_00340"
     CDS             <1..626
                     /locus_tag="VF517_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX8051410.1"
                     /translation="VLCLENTHNGGGGRVWPLDHLRAVVAEARRGGLNVHLDGARLMN
                     AVMASGVPPSDWASGFDTAWLDFTKGLGAPVGACMVGSRALIDEAWRLKQMMGGALRQ
                     AGIVAAAGLYALDHNVERLAEDHSNARALAEGLAELPGASLDVSKVETNIVIFEVDDA
                     VRRVGELAEAGVEVTPVGPERIRAVTHLDVDRDAIERALEAFRRVLG"
     gene            797..1060
                     /locus_tag="VF517_00345"
     CDS             797..1060
                     /locus_tag="VF517_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051411.1"
                     /translation="MALVRDAVSARPVRCRIERLEPGAPGIYACTAGRNADPGAGSGP
                     GPGPDPGPRRLRVEWQHYGTGAYTIRALPGGRVVARGTLSISQ"
     gene            1074..1523
                     /locus_tag="VF517_00350"
     CDS             1074..1523
                     /locus_tag="VF517_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX8051412.1"
                     /translation="MAGVVESIHIAAKKRQMPQSVERAVAVAGRGLEGDRYYEGKGSF
                     SHWRGERDLTLIEAEALEEASIDLEPGAARRNVVVRGLDLAALVGKRFRVGAVECEAL
                     ELNPPCKHLEKLTEPGVMRALADRGGIRAAIREGGEIAVGDPVEPVG"
     gene            complement(1861..2166)
                     /locus_tag="VF517_00355"
     CDS             complement(1861..2166)
                     /locus_tag="VF517_00355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX8051413.1"
                     /translation="MIVVSARARVKPEAREKAMEAARNMREASRAEPGCQEYNFWFAF
                     DDENELLVFEVWDDQAALDAHFATPHLAEFAQAIPTFVDGMPTITRYVVESAGPMGA"
     gene            complement(2176..2568)
                     /locus_tag="VF517_00360"
     CDS             complement(2176..2568)
                     /locus_tag="VF517_00360"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8051414.1"
                     /translation="MPAPEGFVPFEDGGSFLDLIGPVLVRGEGEDMVFGLDVEERHQN
                     SGGKAHGGLLASLVDFSLGRAVAAADAAGVTASLTTDYLAPVELGDFVEAHTEVEKAG
                     STLAFADCSLRVDGREVVRARAVFAVAG"
     gene            complement(2585..2776)
                     /gene="rpmB"
                     /locus_tag="VF517_00365"
     CDS             complement(2585..2776)
                     /gene="rpmB"
                     /locus_tag="VF517_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX8051415.1"
                     /translation="MPKVCYSCGKGPSFGHSRSHSMVATKRRFDPNLQKVRIEVKGSP
                     RREYVCTRCLKSGKVLKAV"
     gene            complement(2848..4182)
                     /locus_tag="VF517_00370"
     CDS             complement(2848..4182)
                     /locus_tag="VF517_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051416.1"
                     /translation="MPRFAVLLVALFALAAAPAAAQTTLQGGSVQDAVEALRTDPVYV
                     APGAQPGLSAAEQESVERAIAEADAGPVYVAVVEPDALDQAGGDAGELLRQIATGSAR
                     PGTYVLAAGRQLRAGSTRGLLAPGEAGRLAAEAIADHRAEGLAAILRGFVRSVGEARA
                     HGGRAEPSGGGGGGGGGLLLLGLVAIPLALFGFRGARRRREERAALAAELEDVSAEAR
                     RDVVALGDDIRALDLDIEMPGIDPAARDDYSRALAAYERAGQALDRARRPEDFGVVTE
                     AVEEGRYAIVATKARLEGRQPPERRPPCFFDPRHGPSVRDVEWAPPGGAPRPVPACAA
                     DAVTLEEGRDPAFREVMVGGQRRPYWEASPAYAGWAGGYYGGFGGFGFFPGLLFGTML
                     GGSMGGWGGDAYGADYGGGDMGGGGDFGDFGGGFGGGDFGGGGFGDFGGGDF"
     gene            complement(4317..4934)
                     /gene="def"
                     /locus_tag="VF517_00375"
     CDS             complement(4317..4934)
                     /gene="def"
                     /locus_tag="VF517_00375"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX8051417.1"
                     /translation="MADEHTHPTAEVEPEPGELEEAPPLDPEVEARRAAAMSFIRRLG
                     DPVLKSRATDVDRFDDGLRSQVARMAAIMNDALGVGLAAPQLGISQRLLVYRVGQDAP
                     LVALVNPQIEWKGPEEEVFEEGCLSIPGVQVDVPRHVHVRVRAQDEEGEERVVEASGL
                     EARVIQHEIDHLDGVLMLDRTPRDQRKEAMRTLRELERERNERAA"
     gene            complement(4972..7158)
                     /gene="priA"
                     /locus_tag="VF517_00380"
     CDS             complement(4972..7158)
                     /gene="priA"
                     /locus_tag="VF517_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX8051418.1"
                     /translation="MIAKVEPLTPARALRGPFDYLLPVGMSEVDVGSMLIVPFGRRRL
                     LGVVVDLAERSELPPERLVEPLAALDRGVPPELVRLGLWVAEEYCSTPARGLALVLPP
                     GAGTGALPRVGIRRALVARITKVGQAALVSDERLGLRQRAALERLDQEPATAAELARA
                     AGCTHSTLRSLEARGLIELERAEQRRARPVSVAVGAVGPDEPDLTGAQRAALDEIERR
                     MAAPDPGGGLLLHGVTGSGKTEVYLRAAAAALARGRSAIVLVPEIALTPQTASRFRRR
                     FGDTVAILHSKLGAGERHDEWLRLRRGEARVSVGPRSAVFAPVPDLGLIVVDEEHDGS
                     YKQEGDPRYDARRVAERRAEQAGAVLVAGTATPRPESSVRLARVSLPDRVDGRGMPPV
                     ELAPMLDAPTLLHPHSRAALEGVRERGEKAIVLLNRRGWSNFLSCRSCGRVWECPNCD
                     VSLVLHRSAARVACHHCGHDERVPSECPDCRSVSVARHGFGTERVESELEELMAPLPI
                     MRLDADVAAGRTAVQDLLRSFDEASSGVLLGTQMVAKGHDFPDVTLGLVLDADSTLRF
                     PDFRAEERTFALVAQLAGRSGRGPRGGRVVVQTLDPSARSLRYAAAHDSDGFVADELE
                     RRRALTYPPFSTLIRVVCLSPEPGPEIAAAMAVRERIDLPGVSALGPAALFRLKGRER
                     AQVLIKAPADDAGRAGAVRAVRRAVEALAGTKEHKGVGWSVDVDPQ"
     gene            7202..7705
                     /locus_tag="VF517_00385"
     CDS             7202..7705
                     /locus_tag="VF517_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX8051419.1"
                     /translation="MDLNPGEVVIFEGRPSWRAILGFYILGLLAVAAAFAIGLLAADA
                     GIGAAAGAVVLVIVLLWGWAKRYATRYAITNRRLHIRRGILSKSVEETRLDRVQDVKI
                     KQGVGQRLLGIGTVDFDTSAESGAGFRFQGIARPDHVARQVDRTIHEADAPAPPIPPP
                     PPPQPPA"
     gene            complement(7702..8334)
                     /locus_tag="VF517_00390"
     CDS             complement(7702..8334)
                     /locus_tag="VF517_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX8051420.1"
                     /translation="MHVAELWRYPVKSMRGERLDTTEVRPDGFAGDRLVQVREGDQVL
                     TGRTRPGLLGLSATLSADGRALVDGAAWDSPEAAAAVREVAGEGAALVADGVSRFHDT
                     TPLLVATDGMIEGFGIDFRRLRPNVVVGGVEGLSERDWEGRRLRVGDVVIRLDHLCER
                     CVMTTFDPDTQEQDPEVLRRINAELGGLAAINSSVEVPGRIAVGDPVELV"
     gene            complement(8366..8824)
                     /locus_tag="VF517_00395"
     CDS             complement(8366..8824)
                     /locus_tag="VF517_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051421.1"
                     /translation="MKTLRLTPLAVIAALALAAPAQADTPADGHTTGAGTVTDLGNPF
                     APVRINVNASSVDGVAQGRVSVRNGEFRFVGDVQCYVQVGNTVEIAGQVVKSKTLDGG
                     YYALSIQDNGETGDLVSLNASADIPLLCGQAGMPVFALEDGNLTVHAGLE"
     gene            complement(8879..10126)
                     /gene="metK"
                     /locus_tag="VF517_00400"
     CDS             complement(8879..10126)
                     /gene="metK"
                     /locus_tag="VF517_00400"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX8051422.1"
                     /translation="MSTQPLSDNEYLFTSESVTEGHPDKMADQISDGVLDAVMREDPT
                     GRVACETLVNTGLVVVSGEISTSTYVDVPKIARETIRKIGYDNASYGFDCDTCAVITA
                     IDEQSPDIAQGVDEAYEMRTDPADDDELDKAGAGDQGMMFGYATRETKELMPMPIALA
                     HKLAHRLAEVRRGDVMPYLRPDGKTQVTVRYENGRPIEIEKVLISTQHKPGVDSQTLI
                     KPDLFEHVLHPVLPSELYDEKKLLDGFLVNPTGKFEIGGPMGDCGLTGRKIIVDTYGG
                     AARHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLADRCEVQVAYAIGVARPVSVM
                     AETFGTERVSRGKIAQLIDEHFDLRPAAFRQELRLHRPIYQKTAAYGHFGRDDHDFTW
                     EKTDKADALREAAGLGSSEAVTA"
     gene            complement(10173..11486)
                     /gene="der"
                     /locus_tag="VF517_00405"
     CDS             complement(10173..11486)
                     /gene="der"
                     /locus_tag="VF517_00405"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX8051423.1"
                     /translation="MALPRVAVVGYPNVGKSTLVNRLTGSREAVVHEEAGVTRDRKEL
                     EADWNGRHFLLVDTGGVDLADDDELARLVQGQARAARDGAEAIVLVVDARAGVRPGDD
                     ELARELRGAPVPVIVAANKIDSAAAIPEAAEFHRLGLGEPVPVSAAQGLGTGDLLDRV
                     AEALEDAPEPPPDDETPRIAVIGRPNVGKSSLVNAWLGQERVIVSDRAGTTRDAVDTR
                     LEVDGREIVLVDTAGLRRRGKIAGTVDWYAQLRSERAAERADVAVVLCDASEALTTED
                     LRVGELAMQKECATLLALNKWDVGRTELEDAKERAQARLRQRPEVLAVSAKTGRGVRR
                     LLLHALELADRVNVRIPTSELNRFLSDVQSAREPPVVRGKRLKFLYMTQYDTRPPRFS
                     VQVSDRGRLTRDYGFMLENRLRERYKLHGVPLVIDYHGREEKRPR"
     gene            complement(11486..12106)
                     /gene="cmk"
                     /locus_tag="VF517_00410"
     CDS             complement(11486..12106)
                     /gene="cmk"
                     /locus_tag="VF517_00410"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX8051424.1"
                     /translation="MVVAIDGPAGAGKSTVARGVAERIGFGYLDSGAMYRAVALAQIE
                     QGGDAAERAAGARIELGERVRLDGRDVSEAIRRPDVSEAASRIATSRAVREAMVAKQR
                     ELVASGDWVAEGRDIGTVVAPGAELKIFLTASPEARARRRAADLGVDWRVVLRDQGLR
                     DAQDEQREHSPLRPAPDAIELDTTDRPAEDVIAQIAGLVADARRNS"
     gene            complement(12117..13457)
                     /gene="aroA"
                     /locus_tag="VF517_00415"
     CDS             complement(12117..13457)
                     /gene="aroA"
                     /locus_tag="VF517_00415"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX8051425.1"
                     /translation="MRRRFEPSGSLRGRCVPPPDKSISHRAALFAAMTDEPVSVRNYL
                     AAEDTLSTLDVVRALGAAVELEPTTVGGGTGAAASAGGLSVLVRGVGLGTAAPTTGGL
                     LDVGNAGTLLRILPGWLAGQPGGVWTLDGDASIRRRPIDRVADPLRLMGADIEAREGR
                     FTPLTIRGTQLGGIDYALPVASAQVKSCLLIAGLLAEGATTLTEPQRSRDHTERILTR
                     ARAPFNRSGDILTVSTADELELDDVVVPGDPSSAAFLAAAALIVPGSRLVIESVALNW
                     TRTAFFRIAERMGGVVLGDLEEPAEGVPADEPVGEIDVAQAPLEGTVVEADEVPLAID
                     ELPLVALLGCFAEGETVVRGAEELRVKESDRIAGVVDGLGALGADIEATPDGFVVRGT
                     GGLEGGVLEARGDHRLAMLGAVAGLASRSGVEVDGIEAAAISYPGFEADLASLA"
     gene            complement(13454..>14489)
                     /locus_tag="VF517_00420"
     CDS             complement(13454..>14489)
                     /locus_tag="VF517_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX8051426.1"
                     /translation="LIGGSIGLAARGEGHEVTGWDPDPGALERATGSGALSRAAASVE
                     EAAGVADIVFACAPVSALPGLVRQALGAAGPQTLVTDVGSTKRAIASIDDERFVGGHP
                     IAGAETAGVENARATLFHGATWYLTPSERSSGVLYERLHSLLARLGARPVAIDAEAHD
                     RLLAAVSHLPHVIANVLVSQVAARLRDQGEGLPRVGPSFRDVTRVAGANSAIWTDIYV
                     SNADAIADEIEALARGLGEVAGSLRDREAMAEWNERAAEDRRALLEADLAGGPVHELQ
                     VSVPNRPGIVAQVALALGRGGVNIADMALAPAPDNRSGAITLWITGDGAGARAAELIE
                     SLGFPVADVG"
BASE COUNT         1870 a         5419 c         5341 g         1859 t
ORIGIN      
        1 aggtcctctg tctcgagaac acccacaacg gcggcggcgg tcgcgtctgg ccgctcgacc
       61 accttcgcgc cgtcgtcgca gaagcgcggc gcggcgggct caacgtccac ctcgacggcg
      121 cgcgcctgat gaacgccgtg atggcctccg gcgtgccgcc ctccgactgg gcgtcgggat
      181 tcgacacggc ctggctggac ttcaccaagg ggctgggcgc gccggtcggc gcctgcatgg
      241 tgggctcgcg ggcgctgatc gacgaggcgt ggcgactcaa gcagatgatg ggcggcgcgc
      301 tgcgccaggc ggggatcgtg gcggcggccg ggctgtacgc gctcgaccac aacgtggagc
      361 ggctcgccga ggaccactcg aatgcgcggg cgctggcgga gggcctggcg gagcttccgg
      421 gggcgtcttt ggacgtctcg aaggtcgaga cgaacatcgt gatcttcgag gtggacgacg
      481 cggtcaggcg cgtgggagag ctggccgagg cgggcgtgga ggtcacgccg gtggggccgg
      541 agcgcatccg cgcggtcacg cacctggacg tggaccgcga cgcgatcgag cgggcgctgg
      601 aggctttccg acgcgtgctg gggtagtcgc gctcgccgtc ttggccctcg cgacggcttc
      661 ggctttcgcg gtcggctgct ccgaggagga gggcgagcgg tctccgcccg cctccactcc
      721 cccgcggccg tccccggccc cgtccgcggc tccggagcgg ggccgggggc tcgacagaag
      781 caatgaggag gaggtcgtgg cgctcgtgcg cgacgcggtc tccgcgcggc cggttcggtg
      841 ccgcatcgag cggctcgagc cgggtgcgcc ggggatctat gcgtgcaccg ccggccgcaa
      901 cgcggacccg ggcgcaggtt cgggcccagg cccgggtccg gacccgggcc cccggcggct
      961 tcgcgtcgag tggcagcact acggcacggg cgcctacacg atccgcgcgc tgccgggggg
     1021 ccgcgtggtc gcgcgcggga cgctgtcgat cagccagtag cgtggagggc cgcatggccg
     1081 gagtagtcga gtcgatccac atcgcggcga agaagcggca gatgccgcag tcggtcgagc
     1141 gcgcggtcgc cgtggccggg cgcgggctcg agggcgaccg ctactacgag ggcaagggca
     1201 gcttcagcca ctggcgcggc gagcgcgacc tgacgctgat cgaggccgag gcgctcgagg
     1261 aggcctcgat cgatctggag ccgggcgcgg cgcggcgcaa cgtggtggtg cgcgggctcg
     1321 acctcgcggc gctggtcggc aagcgcttcc gggtgggagc ggtcgagtgc gaggcgctgg
     1381 agctcaaccc gccctgcaag cacctcgaga aactgaccga gccgggcgtg atgcgcgccc
     1441 tggccgaccg cggcggaatc cgagcggcga tccgggaggg cggcgagatc gcggtgggcg
     1501 acccggtgga gccggtcggc tagagccggc ggcggctcgg ggagcccggc cggctcgggg
     1561 agcccagccg gctcggagag cccagtcggc acggcgagcc cggccggctc gggagccccc
     1621 gccgcccggc agcccgcggg gatcgccgtg ttacagaacg cgcatcccgt aacacggtgt
     1681 ccgtgggttt cttcggcatt cctgacttat gggtctataa ggcccgcagg gcaggaatgc
     1741 cgtccgaaac aggcgaccgg agggttacag gagtgttacg gccggctgtg gcgccgagcg
     1801 ggacggccgg ctgtggcgcc gagcgggacg gccggctgcg gcgccgagtg ggccagccgg
     1861 ctaggcgccc atcgggccgg ccgactcgac cacgtagcgc gtgatcgtgg gcatcccgtc
     1921 gacgaaggtc gggatcgcct gggcgaactc ggccaggtgc ggggtagcga agtgggcgtc
     1981 gagggcggcc tggtcgtccc agacctcgaa cacgagcagt tcgttctcgt cgtcgaacgc
     2041 gaaccagaag ttgtactcct ggcagcccgg ctcggcacgc gatgcctcgc gcatgttgcg
     2101 cgcggcttcc atggccttct cccgcgcctc gggcttcact cgtgcgcggg cgctgacgac
     2161 gatcacggcg cgatcctaac ccgcgacggc gaagaccgcg cgggcgcgca cgacctcgcg
     2221 accgtccacg cgcagcgagc agtcggcgaa ggccagggtc gagccggcct tctcgacctc
     2281 ggtgtgggcc tccacgaagt cgccgagctc gaccggggcg aggtagtcgg tggtgaggct
     2341 ggcggtcacc ccggcggcgt cggcggcggc cacggcgcga ccgagcgaga agtccacgag
     2401 ggaggcgagc aggccgccgt gcgccttgcc gccggagttc tggtggcgtt cctcaacatc
     2461 gaggccgaac accatgtcct ccccctcccc gcgcacgagc accggaccga tcaggtcgag
     2521 gaacgagccg ccatcctcga acgggacgaa cccctcgggg gcgggcacta ggccgccgcc
     2581 ccgatcagac ggccttgagg accttgccgg acttcaggca gcgcgtgcag acgtactcgc
     2641 ggcggggcga gcccttgacc tcgatgcgca ccttctggag gttcgggtcg aaccggcgct
     2701 tggtggcgac catcgagtgg ctgcggctgt gcccgaaaga cgggcccttt ccgcacgagt
     2761 aacagacctt cggcacggcc ggtgaggata gcggcgggcg gaaagcccgc gggggcggcg
     2821 cggcgcgaag ccgccgcggg cgcggcgcta gaagtccccg cctccgaagt cgccgaagcc
     2881 tccgcctccg aagtcgcctc cgccgaagcc ccctccgaag tcgccgaagt ctccgccgcc
     2941 gcccatgtcg ccgccgccgt aatcggcgcc gtaggcgtcg ccgccccagc cgcccatgga
     3001 tccgccgagc atcgtgccga acagcaggcc cgggaagaag ccgaagccgc cgaatccgcc
     3061 gtagtacccg cccgcccagc cggcgtacgc gggcgaggcc tcccagtacg ggcgccgctg
     3121 gcctccgacc atgacctcgc ggaaggccgg gtcgcggccc tcctccagag tcaccgcgtc
     3181 ggcggcgcag gcgggcacgg gccgcggggc gccgccgggc ggcgcccact ccacgtcgcg
     3241 caccgaagga ccgtgacgcg ggtcgaagaa gcacggcgga cgccgctcgg gcggctggcg
     3301 gccctcgagc cgcgccttcg tcgcgacgat cgcgtagcga ccctcctcga ccgcctctgt
     3361 gaccacgccg aagtcctccg gacgccgggc gcggtcgagc gcctgcccgg cccgctcgta
     3421 cgccgccagc gcgcggctgt agtcgtcgcg ggcggcgggg tcgatcccgg gcatctcgat
     3481 gtcgaggtcg agcgcgcgga tgtcgtcgcc gagcgcgacc acgtcgcggc gcgcctcggc
     3541 cgagacgtcc tcgagctccg cggcgagcgc ggcgcgctcc tcgcggcggc gcctcgcccc
     3601 gcggaacccg aacagcgcca gcggtatcgc gaccaggcca aggaggagca ggccgccccc
     3661 accaccgcca ccgccaccgg acggctcggc tcggccgccg tgcgcacgcg cctcacccac
     3721 gctccggacg aagccgcgca ggatcgcggc gagcccctcg gcgcggtggt cggcgatcgc
     3781 ctccgccgcg aggcggcccg cctcgccggg ggccagcagc ccgcgcgtgc tccccgcgcg
     3841 aagctggcgt ccggccgcca acacgtacgt gcccgggcgt gcggagccgg tcgcgatctg
     3901 gcgcagcagc tcacccgcgt cgccgccggc ctggtcgagc gcatccggct ccacgacggc
     3961 gacgtacacg ggacccgcat cggcctcggc gatcgcgcgc tccacggact cctgctcggc
     4021 ggcactgagg cccggctgcg cgcccggagc cacgtagacg ggatcggtgc gcagcgcctc
     4081 cacggcatcc tggacggagc cgccctggag ggtcgtctgc gcggcggccg gagcggcggc
     4141 gagcgcgaac agcgccacga ggagcacggc gaaacggggc acaccccgta gctacccgct
     4201 caggggtggc gaaatcccgg gaccgcggtt aggttggagc cagagcttgc aacagggcgg
     4261 actcgcacct cgtcctcggc agccgcgacc gccccggggg gctggtggtc gcggcgctag
     4321 gccgctcgtt cgttgcgctc gcgctccagc tcgcgcagcg tccgcatcgc ttccttgcgc
     4381 tggtcgcgcg gggtgcggtc gagcatgagc acgccgtcga ggtggtcgat ctcgtgctgg
     4441 atcacgcgcg cctcgagccc ggacgcctcg accacgcgct cctcgccctc ctcgtcctga
     4501 gcgcggacgc gcacgtggac gtggcggggc acgtcgacct ggaccccggg gatcgacagg
     4561 cagccctcct cgaagacctc ctcctcgggc cccttccact cgatctgggg gttgaccagg
     4621 gccacgagcg gcgcgtcctg ccccacgcgg tacaccagca gccgctggga gatcccaagc
     4681 tggggcgcgg cgagcccgac gccgagcgcg tcgttcatga tcgcggccat ccgcgcgacc
     4741 tggctgcgca gcccgtcgtc gaagcggtcc acgtcggtgg cgcggctctt caggacggga
     4801 tcgcccaggc gcctgatgaa cgacatcgcc gcggcccggc gcgcctcgac ctcgggatcg
     4861 agcggcggag cctcctcgag ctcgccgggc tccggctcga cctcggcggt cggatgagtg
     4921 tgctcgtcgg ccacggcgga cgagtttagg gcgtgaggag gggtcgcgct gctactgcgg
     4981 gtccacgtct accgaccagc cgaccccctt gtgctccttc gtgccggcca gcgcctccac
     5041 cgcgcgccgc accgcacgca cggcgcccgc gcgccccgcg tcgtccgccg gcgccttgat
     5101 cagcacctgc gcccgctcgc gccccttgag ccggaacagc gcggccgggc cgagcgcgct
     5161 cacgccggga aggtcgatcc gctcgcgcac cgccatcgcc gccgcgatct ccggccccgg
     5221 ctcgggcgag aggcagacga cccggatcag ggtggagaag ggcgggtagg tgagcgcacg
     5281 gcggcgctcg agctcgtcgg cgacgaagcc gtcggagtcg tgggcggccg cgtagcgcag
     5341 cgagcgcgcc gacgggtcga gcgtctgcac caccacgcgc cccccgcgcg gcccgcgccc
     5401 gctgcgtccc gccagctgcg cgaccagcgc gaacgtgcgc tcctcggcgc ggaagtcggg
     5461 gaaccgcagc gtcgagtcgg cgtccagcac gagcccgagc gtgacgtcgg ggaagtcgtg
     5521 gcccttggcg accatctgcg tgccgagcag aacgcccgag gacgcctcgt cgaacgagcg
     5581 cagcaggtcc tgcaccgcgg tgcgcccggc ggccacgtcc gcgtcgagcc gcatgatcgg
     5641 cagcggcgcc atcagctcct cgagctcgct ctccactcgc tcggtgccga agccgtgccg
     5701 cgcgaccgac accgaccggc agtccgggca ctcgctcggc acccgctcgt cgtgcccgca
     5761 gtggtggcag gccacgcgcg ccgccgagcg gtggagcacg agcgacacgt cgcagttggg
     5821 gcactcccac acccgcccgc acgaccggca cgagaggaag ttcgaccagc cgcggcggtt
     5881 gagcagcacg atcgccttct cgccgcgctc gcgcaccccc tcgagcgccg cgcgcgaatg
     5941 cgggtggagc agggtgggcg cgtcgagcat cggggcgagc tccaccggcg gcatgccgcg
     6001 cccgtcgacc cggtcgggca gcgacacgcg cgccagccgc acgctgctct ccggccgcgg
     6061 cgtggccgtg cccgcgacaa gcaccgcacc cgcctgctcg gccctgcgct cggccacgcg
     6121 ccgagcgtcg tagcgcgggt cgccctcctg cttgtacgag ccatcgtgct cctcgtccac
     6181 cacgatgagg ccgaggtcgg gcaccggcgc gaagaccgcc gagcgcgggc cgacggagac
     6241 acgcgcctcg ccgcggcgca gcctgagcca ctcgtcgtgg cgctcgccgg cgccgagctt
     6301 cgagtggagg atcgcgaccg tgtcgccgaa gcgccgccgg aagcgcgacg cggtctgcgg
     6361 cgtgagcgcg atctccggca cgagcacgat cgccgagcgc ccgcgggcga gcgcggcggc
     6421 ggccgcgcgc aggtacacct cggtcttgcc gctgccggtg acgccgtgca ggagcagccc
     6481 acctccgggg tccggggcgg ccatgcgccg ctcgatctcg tcgagcgccg cgcgctgggc
     6541 gcccgtgagg tcgggctcgt ccggcccgac ggcgcccacg gcgacgctga ccggccgcgc
     6601 gcgccgctgc tcggcgcgct cgagctcgat cagcccgcgc gcctccagcg accgcagcgt
     6661 cgagtgggtg cagccggccg cccgcgccag ctcggcggcg gtggcgggct cctggtcgag
     6721 ccgctcgagc gcggcgcgct ggcgcagtcc gagccgctcg tccgagacca gcgccgcctg
     6781 accgaccttc gtgatccgcg ccaccagcgc ccggcgaatg ccgacacgcg gcagcgcgcc
     6841 tgttcccgcg cccggcggca gcacgagcgc cagcccgcgc gccggggtcg agcagtactc
     6901 ctcggccacc cagagaccga gccggaccag ctcgggcggc acgccccggt cgagcgccgc
     6961 aagcggctcc acgagccgct ccgggggcag ctcgctgcgc tccgccaggt ccacgaccac
     7021 gcccagcagg cgcctgcggc cgaacgggac gatcagcatc gagccgacgt ccacctcgct
     7081 catccccacc ggcagcaggt agtcgaacgg gccgcgcagg gcgcgggcgg gggtcagcgg
     7141 ctcgaccttg gcgatcacgt cttcgaccgt agggtcgggg ccggacggta ggctgcgcca
     7201 catggacctg aatcccggcg aggtcgtgat cttcgagggg cgcccctcgt ggcgcgcgat
     7261 cctcggcttc tacatcctcg ggctgctggc ggtcgccgcg gccttcgcga tcggcctgct
     7321 ggccgccgac gcgggcatcg gggccgccgc gggcgccgtc gtgctcgtaa tcgtgctcct
     7381 ctggggctgg gccaagcgct acgccacgcg ctacgccatc acgaaccgcc ggctccacat
     7441 ccgccgcggc atcctgagca agagcgtcga ggagacgcgg ctcgaccgcg tacaggacgt
     7501 gaagatcaag cagggagtcg gacagcggct gctcggcatc gggacggtcg acttcgacac
     7561 gtcggccgag agcggagccg gcttccgctt ccaggggatc gcgcggcccg accacgtcgc
     7621 ccggcaggtc gaccggacga tccacgaggc ggacgccccg gcgccgccca tcccgccgcc
     7681 acccccgccg cagccgccgg cctagaccag ctcgaccgga tcgccgacgg cgatccgccc
     7741 tggcacctcc acgctcgagt tgatcgccgc gaggccaccc agctcggcgt tgatgcgccg
     7801 cagcacctcg gggtcctgct cctgcgtgtc cgggtcgaag gtcgtcatca cgcagcgctc
     7861 gcagaggtgg tcgagccgga tcacgacgtc gccgacgcgc agccggcgac cctcccagtc
     7921 gcgctcgctg aggccctcga cgccgcccac gaccacgttc ggccgaagcc ggcggaagtc
     7981 gatcccgaag ccctcgatca tcccgtcggt ggccaccagc agcggcgtgg tgtcgtggaa
     8041 gcgcgagacc ccgtccgcga ccagcgcggc accctccccg gccacctcgc gcacggccgc
     8101 ggccgcctcc ggcgagtccc aggccgcccc gtccacgagc gccctgccgt cggcggagag
     8161 cgtggccgag aggcccagca ggcccggccg cgtcctcccg gtcaggacct ggtcgccctc
     8221 acgcacctgc accagccggt cgccggcgaa gccgtccggt cggacctccg tggtgtcgag
     8281 ccgctcccct cgcatcgact tgaccggata gcgccagagc tccgcgacgt gcatcgcggc
     8341 cagtgaagca gacggctggg gggagctact cgagcccggc gtggacggtc aggttgccgt
     8401 cctcgagggc gaagaccggc atgccggcct gcccgcagag cagggggatg tcggccgacg
     8461 cgttgagcga caccaggtcg cccgtctcgc cgttgtcctg gatcgagagc gcgtagtagc
     8521 cgccgtcaag ggtcttggat ttcacgacct gcccggcgat ctcgacggtg ttgcccacct
     8581 gcacgtagca ctggacgtcg cccacgaagc ggaactcccc gttgcggacc gacacgcggc
     8641 cctgggccac gccgtccacg ctgctcgcgt tcacgttgat ccgcaccggc gcgaaggggt
     8701 tgccgaggtc cgtgaccgtg ccggccccgg tcgtatggcc gtcggcgggc gtatcggcct
     8761 gggcgggcgc ggcgagcgcg agggccgcga tgacggccag tggggtgagc ctgagtgtct
     8821 tcacccgccc actatcggcc cggcggcgcg cggccgcaag accgggtgga cgtttcgact
     8881 aggcggtgac ggcctcggac gagccgaggc ccgccgcctc gcgcagagcg tcggccttgt
     8941 cggtcttctc ccaggtgaaa tcgtggtcgt cgcggccgaa gtggccgtac gccgcggtct
     9001 tctggtagat cggccggtgc agccggagct cctggcggaa cgccgccggg cgcaggtcga
     9061 agtgctcgtc gatgagctgg gcgatcttcc cgcggctgac ccgctcggtc ccgaacgtct
     9121 cggccatcac ggagacgggc cgggcgacgc cgatcgcgta ggcgacctgg acctcgcagc
     9181 ggtccgcgag cccggcggcg accacgttct tcgccacgta acgggccgcg taggcggcgg
     9241 accggtcgac cttcgacggg tccttgcccg agaacgcgcc gccaccgtga cgggcggcgc
     9301 cgccataggt gtcgacgatg atcttgcgcc cggtgaggcc gcagtcgccc atcgggccgc
     9361 cgatctcgaa cttgcccgtc gggttgacga ggaagccgtc gagcagcttc ttctcgtcgt
     9421 agagctcgct cgggagcacg gggtgcagca cgtgctcgaa gaggtcgggc ttgatgaggg
     9481 tctgggagtc gacgcccggc ttgtgctggg tcgagatcag gaccttctcg atctcgatcg
     9541 ggcggccgtt ctcgtagcgg acggtgacct gggtcttgcc gtcggggcgc aggtacggca
     9601 tcacgtcgcc gcggcggacc tcggcgagcc ggtgcgcgag cttgtgcgcg agcgcgatcg
     9661 gcatcggcat gagctccttc gtctcacgcg tggcgtagcc gaacatcatc ccctggtcgc
     9721 ccgcgccggc cttgtcgagc tcgtcgtcgt cggccgggtc ggtgcgcatc tcgtacgcct
     9781 cgtcgacgcc ctgggcgatg tccggcgatt gctcgtcgat cgcggtgatc acggcgcagg
     9841 tgtcgcagtc gaacccgtag ctggcgttgt cgtagccgat cttgcggatc gtctcgcgtg
     9901 cgatcttcgg cacgtccacg taggtggagg tcgagatctc gccggagacc accacgagcc
     9961 cggtgttgac gagcgtctcg caggccacgc ggccggtcgg gtcctcgcgc atgaccgcgt
    10021 cgagcacgcc gtcggagatc tggtcggcca tcttgtccgg atgaccctcg gtgacggact
    10081 ccgaggtgaa caggtactcg ttgtcggaca gtggctgggt gctcattggt atgcggacgc
    10141 gctccggaat cgggttctga gaccgcccga ggttagcgag ggcgcttctc ctcgcggccg
    10201 tgatagtcga tcacgagcgg cacgccatgc agcttgtagc gctccctgag ccggttctcg
    10261 agcatgaagc cgtagtcgcg cgtgagccgc ccgcgatcgg acacctggac gctgaagcgc
    10321 ggcggccgcg tgtcgtactg ggtcatgtag aggaacttga gccgcttgcc gcggaccacc
    10381 ggcggctcgc gtgccgactg gacgtcggag aggaagcggt tgagctccga ggtcgggatc
    10441 cggacgttca cgcggtcggc cagctcgagc gcgtgcagca gcagccgccg cacgccgcgg
    10501 ccggtcttgg ccgacaccgc cagcacctcc gggcgctggc gcagacgggc ctgcgcacgc
    10561 tccttggcgt cctcgagctc ggtgcggccc acgtcccact tgttgagggc gagcagcgtg
    10621 gcgcactcct tctgcatcgc gagctcgccc acacgcaggt cctcggtggt gagcgcctcg
    10681 gaggcgtcgc agagcaccac cgccacgtcg gcgcgctcgg ccgcgcgctc cgagcgcagc
    10741 tgcgcgtacc agtcgacggt cccggcgatc ttgccgcgcc ggcgcagccc ggcggtgtcc
    10801 accagcacga tctcgcgccc gtccacctcg agccgcgtgt ccaccgcgtc gcgcgtggtg
    10861 ccggcccggt cggagacgat cacgcgctcc tggccgagcc aggcgttcac gagcgacgac
    10921 ttgccgacgt tcggccgccc gatcaccgcg atgcgcgggg tctcgtcgtc gggcggcggc
    10981 tcgggagcgt cctcgagcgc ctctgcgacg cggtcgagca ggtcgcccgt gccgagcccc
    11041 tgcgccgccg agaccggcac tggctcgccg agcccgaggc ggtggaactc ggccgcctcc
    11101 gggatcgcgg cggcgctgtc gatcttgttg gcggccacga tcaccggcac cggcgcgccg
    11161 cgcagctcgc gggccagctc gtcgtcgccc ggtcgcacgc cggcccgcgc gtcgaccacc
    11221 agcacgatcg cctcggcccc gtcgcgcgcc gcgcgcgcct ggccctgcac caggcgggcc
    11281 agctcatcgt cgtcggcgag atctaccccg ccggtgtcga ccagaaggaa gtggcgcccg
    11341 ttccagtcgg cctcgagctc cttgcggtcg cgcgtgacgc cggcctcctc gtgcacgacg
    11401 gcctcgcgcg agccggtgag ccggttgacc agcgtggact tgccgacgtt ggggtagccg
    11461 acgacggcca cgcggggcag cgccatcagg aattgcggcg agcgtccgcg acgagccctg
    11521 cgatctgggc gatcacgtcc tcggccggcc ggtcggtcgt gtcgagctcg atcgcgtccg
    11581 gcgcgggccg cagcggggag tgctcgcgct gctcgtcctg ggcgtcgcgc aggccctggt
    11641 cgcgcagcac cacgcgccag tccacgccga ggtccgcggc gcggcggcgg gcgcgcgcct
    11701 ccgggctcgc cgtgaggaag atcttcagct ccgcgcccgg tgccaccacc gtgccgatgt
    11761 cgcgcccctc ggcgacccag tcgccggagg ccaccagctc gcgctgcttg gcgaccatcg
    11821 cctcgcgcac ggccctgctc gtggcgatgc gcgaggcggc ctcgctcaca tcggggcggc
    11881 gaatcgcctc ggacacgtcg cggccgtcga gccgcacgcg ctcgcccagc tcgatccgcg
    11941 cgccggccgc gcgctcggcg gcgtcgccgc cctgctcgat ctgcgcgagc gcgaccgccc
    12001 ggtacatcgc tcccgagtcg aggtagccga agccgatccg ctccgcgacc ccgcgcgcga
    12061 cggtgctctt ccccgcgccc gcgggcccgt cgatggcgac gaccatggcg gggaggctat
    12121 gccagggacg ccaggtccgc ctcgaagccc gggtaagaga tcgcggcggc ctcgatgccg
    12181 tcgacctcca ccccgctccg cgacgcgagt cccgcgaccg ctcccagcat cgccagccgg
    12241 tggtcgccgc gcgcctcgag caccccgccc tcgagcccgc cggtgccccg caccacgaac
    12301 ccgtccgggg tggcctcgat gtcggcgccg agcgcgccga gcccgtccac cacgccggcg
    12361 atccggtcgg actccttcac gcgcagctcc tccgcgccgc ggaccacggt ctcgccctcc
    12421 gcgaagcagc cgagcagcgc cacgagcggc agctcgtcga tcgccagcgg cacctcgtcc
    12481 gcctcgacca ccgtgccctc gagcggcgcc tgggccacgt cgatctcgcc gaccggctcg
    12541 tccgccggaa cgccctccgc cggctcctct aggtcgccca gcaccacgcc acccatgcgc
    12601 tcggcgatcc ggaagaacgc ggtgcgggtc cagttgagcg ccacgctctc gatcacgagc
    12661 cgcgagcccg gcacgatcag cgcggcggcg gccaggaacg ccgcggagct cgggtcgccg
    12721 ggcacgacga cgtcgtcgag ctcgagctcg tccgccgtcg acacggtcag gatgtccccc
    12781 gagcggttga acggcgcgcg ggcgcgggtg aggatgcgct cggtgtggtc gcggctgcgc
    12841 tgcggctcgg tgagcgtggt cgcgccctcg gccaggagtc ccgcgatcag caggcaggac
    12901 ttgacctgcg cgctcgccac cggcagcgcg tagtcgatgc cgccgagctg cgtgcccctg
    12961 attgtgagcg gggtgaagcg gccctcgcgc gcctcgatgt cggcgcccat gaggcgcagg
    13021 gggtcggcga cgcggtcgat cgggcggcgg cggatcgagg cgtcgccgtc gagcgtccat
    13081 acgccgccgg gctgccccgc gagccagccg ggcaggatcc gcagcagcgt gccggcgttg
    13141 cccacgtcga gcaggccacc ggtggtcggc gcggcggtgc cgagcccgac gccgcgcacc
    13201 agcacgctca gcccgccggc ggacgctgcg gcgcccgtgc ccccgccgac cgtcgtcggc
    13261 tccagctcga cggcggcccc gagcgcgcgg accacgtcga gcgtcgagag cgtgtcctcg
    13321 gccgcgaggt agttgcggac gctcaccggt tcgtcggtca tggcggcgaa cagcgcggcg
    13381 cggtgcgaga tcgacttgtc cgggggcggc acgcagcgcc cgcgcagcga cccggagggc
    13441 tcgaagcggc gcctcacccg acgtccgcga ccgggaagcc gagcgactcg atcagctccg
    13501 cggcgcgcgc tccggcgccg tcaccggtga tccagagcgt gatcgcaccg gagcggttgt
    13561 ccggcgccgg cgcgagcgcc atgtccgcga tgttcacgcc gccgcggccg agtgcgagtg
    13621 ccacctgcgc gacgataccc ggccggttcg gcaccgagac ctggagctcg tgcacggggc
    13681 cgccggcgag gtcggcctcc agcagcgcgc ggcgatcctc cgccgcccgc tcgttccact
    13741 cggccatcgc ctcgcggtcg cgcaggctgc cggcgacctc acccagcccg cgcgccagcg
    13801 cctcgatctc gtcggcgatg gcgtcggcgt tcgagacgta gatgtcggtc cagatcgccg
    13861 agttcgcgcc cgccacgcgg gtcacgtcgc gaaagctggg gccgacccgg ggcaggccct
    13921 cgccctggtc gcgcagccgc gcggccacct gggacacgag cacgttggcg atcacgtgcg
    13981 ggaggtggct cacggcggcg agcaggcggt cgtgggcctc ggcgtcgatc gcgacgggcc
    14041 gcgcgccgag acgggcgagc agcgagtgca ggcgctcgta gaggaccccg gacgagcgct
    14101 cgctcggcgt gaggtaccag gtggcgccgt ggaacagcgt cgcgcgtgcg ttctcgaccc
    14161 ccgccgtctc ggcgccggcg atcgggtggc cgccgacgaa gcgctcgtcg tcgatggagg
    14221 cgatggcgcg cttggtggag ccgacgtcgg tgacgagggt ctgcgggccc gcggcgccga
    14281 gcgcctggcg cactagcccg ggcagcgcgc tcacgggcgc gcaggcgaac acgatgtccg
    14341 ccacgcccgc ggcctcctcc accgacgccg ccgcccggct cagggcgccg gacccggtgg
    14401 cgcgctcgag cgcgccgggg tcggggtccc agccggtcac ctcgtggccc tcgccccgcg
    14461 ccgcgagccc gatcgacccg ccgatcagg
//
LOCUS       DASVPL010000009        14912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_118177, whole genome shotgun sequence.
ACCESSION   DASVPL010000009 DASVPL010000000
VERSION     DASVPL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14912
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118177"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VF517_00425"
     CDS             <1..1300
                     /locus_tag="VF517_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX8051427.1"
                     /translation="DLHPLVDVIVVARGGGSVRDLATFDDERVCRAIRALSTPVVTAI
                     GHTDNNPVCNHVTHAAWVPRHAAERVVPDRRELLRDVDDAAAAARRAAGAQRMRIEEV
                     RARFAALSGRSERVRARRVELAAHGDVLDHAMRGFLDKTMRSLASAREAFARTPARVR
                     EELAVHGQHLVDFRAAARVAARVTAQAHRDASSFEEDLRLGGRRVLGDHRRNYGKAFD
                     RLAESTANETRKRFVREREGLERVGGELGVAARRGLRVAGDRASLFVGEQRAAQRSAA
                     RRRLDARRELVRERAATTARAGRRVARTHRENYGKALDRQVGTMRRSTERALEAERRR
                     LEQERERRGDAATRATRDIRRRLGAVVDLLHASDFRRRGLLPVTGAGGRVIRSIEDVV
                     VGRALTLHLHDGEVEASAERVRPYPALPNDSEDNGREEAQ"
     gene            1297..1617
                     /gene="xseB"
                     /locus_tag="VF517_00430"
     CDS             1297..1617
                     /gene="xseB"
                     /locus_tag="VF517_00430"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX8051428.1"
                     /translation="MSTEAAKAITFSDAYTELKSITDTLNADQVEVDALVPLLRRGKG
                     LEAALRAHLEQVEQEVEAIEAGEGITPYRIVAPEAAAPAAEPAARSLDAAPPNGSVAD
                     DIPF"
     gene            1805..2260
                     /locus_tag="VF517_00435"
     CDS             1805..2260
                     /locus_tag="VF517_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051429.1"
                     /translation="MPLCFIEPQEAIRVVAESIGREARRQLLGEVVRAWVDEIPEDEL
                     EVHYAKAVEDLDDDDLSMLAAWHASLEVGQPVANKDFLLKAFSSLGEHRREALKIAAG
                     FRGDIAFESGVSEEQALDTVLPWLSAARAEELAADAVELYCWDRMGSDN"
     gene            2553..3134
                     /locus_tag="VF517_00440"
     CDS             2553..3134
                     /locus_tag="VF517_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX8051430.1"
                     /translation="MLRRRSRRRRLRRAPATLVLLLIAAVAVSYGDRVGGSGANGGGG
                     APGSSGGSGPTAPTGAETARVERVVDGDTIRLRGVGPVRLIGVDTPETYGGRDECFGP
                     EATRYVEGLLPRGARVRYVVGEDSRDRYGRLLAYLYLRDGASLNHALVAAGYARTLTI
                     PPNDRYAPTFERAEEGARRARHGMWARPGCAGE"
     gene            complement(3234..3857)
                     /locus_tag="VF517_00445"
     CDS             complement(3234..3857)
                     /locus_tag="VF517_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX8051431.1"
                     /translation="MIRAVISDFGGVLTTPLLQSFVAYQDETGVDFRRFGEAMGRIME
                     RDGAHPLFELECGRITEQEFYAVIEAELGGGVSLADFRERYFAHLHPNEPVVEYMRGL
                     RGRGYRMALLTNNVREWEPLWRAKIPAVDEIFEIVVDSGFVGMRKPDPRIYELTLERL
                     GDVAASECVFVDDIDVNCDAARELGMTPVHFVDAGQAIAEVEELLRG"
     gene            complement(3854..4564)
                     /locus_tag="VF517_00450"
     CDS             complement(3854..4564)
                     /locus_tag="VF517_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051432.1"
                     /translation="MTEPAISLGFFDPGQEVHGILRAGVGVLFEGSGGRTLAELPEIA
                     PDGAGGYRARCGDEVDLAFRPFSPRAELGGASARVCRVDGRAGGQLVDCLGTVGETRT
                     PPAWAELDAVRLVSAVFDDEHAVVLAARRPRGAIGHGQELVVAHLLAGGELFGVEEAR
                     LSTVYDAHGRQRSVGLELWLPGEDFPRRASGSVRAGLSLELEELRVDAGVVAWRMEGR
                     DGAGSYEITARAEPDLAA"
     gene            complement(4561..5091)
                     /locus_tag="VF517_00455"
     CDS             complement(4561..5091)
                     /locus_tag="VF517_00455"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX8051433.1"
                     /translation="MPLLALIVIFIVVPLAELYVILKVGDSLGWEPTLLILAADSILG
                     SMLLKSQGRQVWRRFNEATAAGRIPHSEIIDGVLIIFGGAFLITPGFLTDILGLFFLL
                     PPTRALFRRMVRGGIERGTLWGRVGGAAVRAGRERQAAREARNGVPEGYYDVEGTATE
                     VDTDSRRLPRSGPGEP"
     gene            5117..5413
                     /locus_tag="VF517_00460"
     CDS             5117..5413
                     /locus_tag="VF517_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX8051434.1"
                     /translation="MPSVDEVTEALTNVIDPELGLDFVELGLVYEVEVDDSDVHVTFT
                     LTSPGCPIGPQVSEQMKEFVGELDGVESVHPHMTFNPPWTPDLMSEDAKFALGY"
     gene            complement(5352..6797)
                     /locus_tag="VF517_00465"
     CDS             complement(5352..6797)
                     /locus_tag="VF517_00465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8051435.1"
                     /translation="MPGHKDRLTSVDASFLVQEKRSSHMHVGAILLFEGPPPDYTDFL
                     EGIRSRMHLTPRYRQKLAFPRFEMGRPMWVDDPTFNLEYHVRHTALPTPGSVDQLLKL
                     AGRIYSQRLDRSKPLWETWLIQGMDDNRFALITKTHHSMIDGIAGVDLASVLFDIDPV
                     PRKVDPEPWTPRPVPSDAELIAEGVKDVAKAPIGAAQRALRAVQSPGRTLAQAREAVQ
                     ALGEVAWLGVNPAPSIPLNVPIGPHRRIDRVRAELGDLKRVKNELGGTVNDVVLTVVS
                     GALRRWLRSRGVRTEGLELRALVPVSIRAEDEQGQLGNRIAAMRGPLPVYVDDPVERL
                     AIVREAMEGLKQSKQALGAEVIAGLNDFAPPTILAQASRLNFSTRLFNAIVTNVPGPQ
                     FPLYLLGRELQEIVPLAFLPENHAMAVAIMSYNGTLDFGLLGDYDAMPDLDFFADAVR
                     ESLAELLDAAELSSRGRTSRPRSSGPVSTGG"
     gene            6840..7715
                     /locus_tag="VF517_00470"
     CDS             6840..7715
                     /locus_tag="VF517_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX8051436.1"
                     /translation="MTARTLTAAALAAAALALGCGGDDEVEAPAVEPAASVDEGRFDG
                     AAAMRWVRRQLAYGPRPAGSPPSVKLAKRLRDALPNGRYQAVPGGLRNVLGTVPGRDP
                     KRLVVVGAHYDTKDIPGFVGAVDGASGTAVVTQLARTIKPRSARPTIVFALFDGEEAP
                     AGRPDAEFERYGIRGSKVAARALRRAEAMILLDFVGEKDLQLPREANSDPALWRKLRA
                     GAATAGVIDRFPNRTAPPVSDDHTPFIRRGIPSIDLIDFEFECWHETCDDLSQIGEES
                     LDATGEAMVRMLATL"
     gene            7720..8676
                     /locus_tag="VF517_00475"
     CDS             7720..8676
                     /locus_tag="VF517_00475"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX8051437.1"
                     /translation="MPHAPERLLLAAPRGYCAGVDRAVQAVERALELYGPPVYVRKEI
                     VHNKHVVEQLAARGAVFVDEETEVPKGETVVFSAHGVAPSVHANALERSLRTIDATCP
                     LVTKVHVEAKKFAADGYTIVLIGHEGHEEVEGTMGEAPEHIVLVESEADVETLEVADP
                     ERVAFISQTTLSVDETTAIIARLRERFPAIVGPRTDDICYATTNRQMAVRQMARECDL
                     VLVIGSRNSSNSNRLVEVARAFGADSYLIDNEHEVREEWLEGKRTVGITSGASAPEEL
                     VQRLVDFFRERGTEDVSEFQVVQEDVRFMLPKEIRRELAARA"
     gene            complement(8686..9477)
                     /locus_tag="VF517_00480"
     CDS             complement(8686..9477)
                     /locus_tag="VF517_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8051438.1"
                     /translation="MRPPHDEDLYAPEFPKGIEWRNVAFLRMDKLLGRHGVLVEFWDF
                     ARINSLRTLPYLVAWHERYADAGLRVIAVHSPGYSFGRDPDVVSAAIERLGIPFAVAL
                     DPDYAVWRQYGNRGWPGRYVFDRRGILRYFHYGEGDYEGTELAVQEVLREIEPDAELP
                     APLEPFRPEDAPGVLMTPQTADVTLPAERERLQLVRDWTDGEDWIAAADAGASASVRF
                     EAGSAWAVLSQGGLEPGLYETDGTVVAEEAGLRLHGFQFTPVPPE"
     gene            complement(9506..10207)
                     /locus_tag="VF517_00485"
     CDS             complement(9506..10207)
                     /locus_tag="VF517_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051439.1"
                     /translation="MNSNELVLVQGMAATKATLAEWNSDPWGTLRGWLGWSLLTAVGL
                     LVAVYAVARLSTPDPTSLIIPGVQREATFADAEHVLLKNSLVLALHGMACVAGFLAGS
                     AVPQQAQYKTGVNKWVHEKAGPFAIAFVIAATTFSLCTQAYVIGSATSTVAANLGISP
                     LKLLLALSLHAIPELTALFLPLAAWVVASRRGDWHKLLAATVVTVGLAIPVLFIACMV
                     EVYATPDVIRSIAGL"
     gene            10322..11473
                     /locus_tag="VF517_00490"
     CDS             10322..11473
                     /locus_tag="VF517_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051440.1"
                     /translation="MHPFKTSRLGLLAAATALIAVLVAAPGAGAATFEGGPWKIDFKT
                     FTKAKKLKLTTTGANPKSSTGGTFGLGTGQITNNEQASGNISVGSSTSVIQFALGKKK
                     VTLTSLTQKLTAGKGQINAVVNKKGKAVTLFDVASQGKVGPNSTFTKLTLNSSNVSLT
                     KSGASALNKAFGFKGTAVLKAKQKVGTVGFTGERSLTVLSGTSTSLYDKAFGDALKSC
                     DITLSAVQPASGIPADANNPYGGVNLPVNGANGGTLDAETLTGQVNHEGGTSLDRPGP
                     GQPGNTTGKAEYHSPLVNFVFGFSPGSFTLTAFVTNSNNNLPIGSVTGSLDKNLTAEA
                     GTVTLSNGALNLSDAAAGTLSQAQPPLGANCQIPAGSKIGSLNSTFNVG"
     gene            complement(11536..12147)
                     /locus_tag="VF517_00495"
     CDS             complement(11536..12147)
                     /locus_tag="VF517_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX8051441.1"
                     /translation="MRPRTAIAVMVTTAVAFVALLVVVDRARDRANAGSEDSPATRFE
                     GGTMPPGVRAPDFALTDERGRRVSMRELRGRPVAVTFLYTTCEDTCPTEAQQIKGALD
                     LLDRPVPALAIAVDPPRDTPARAREFLAKYDLAGRMRWVLGSRTQLEKLWRGFAIQEQ
                     LPEHEHQARIVLVDGDGFQRVGFPASEATPERLAHDLRELGAN"
     gene            12393..13583
                     /gene="moeB"
                     /locus_tag="VF517_00500"
     CDS             12393..13583
                     /gene="moeB"
                     /locus_tag="VF517_00500"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX8051442.1"
                     /translation="MPTGAEVLRKIKQEIREVDPREVHDATANGNGSAPVLIDVREQH
                     EFEEGHIPGAVHVPRGHLETRIEGRAGDRSQPIVLYCASGNRSAFAAKTLQDLLGYED
                     VASMTGGITLWKDRGFEVETPRALTAEQKTRYSRHILLPEVGLEGQLALLDAKVLLLG
                     AGGLGAPTALYLAAAGVGTLGIVDDDEVDLSNLQRQVIHTTDRIGVSKVDSAEQTIAG
                     LNPDVDVVKYHTRLDASNIMEIIDGYDVIVDGVDNFPTRYLLNDATVRTKTPVVSASI
                     LGFDGQLSVFAPYEGPCYRCLYPTPPPAELAPSCGANGVLGVLPGTMGMLQATEVIKL
                     ITGQGEPLIGRLLLYEALGASFTELKVRRDPECPICSREPESISDDEMGVFPDYEAFC
                     AGAG"
     gene            13593..13865
                     /locus_tag="VF517_00505"
     CDS             13593..13865
                     /locus_tag="VF517_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX8051443.1"
                     /translation="MSNVKIPPVLRSSTGGEKEVSAAGGTVGEVLRALAEAHPDTESQ
                     IFSGDGQLNRYVNVYLNDEDVRVLDGLETPVKDADTLVILPAMAGG"
     gene            13952..>14912
                     /locus_tag="VF517_00510"
     CDS             13952..>14912
                     /locus_tag="VF517_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051444.1"
                     /translation="MFGVGSAHESLPRHGITHLVEHLAMVPLHWTPYASGAWVEDTTT
                     TFWVEGSLAEVGGFLGDVTRSLGELPLDRLDVERDVLASEAADSGSSFQRRLLGLRFG
                     PATYGVANFLELGLRSVSPEEVEDWRRRSFTAGNAAIWMTARPPDDLVLDLPDGPAVR
                     PPEPEPIPGLRLPAQSADWEGDVAFGMLASRSHALAAGGMIAERRAEDRLRVERGLTY
                     GVEWELFPLTAGTAHLFLSADSTDANAVVIRDGIGAVLEELARDGPTEEELERLVAED
                     ERWMHDPGFPGEILDRAAREHLRGAPLEQPGELMEERRRVRPAA"
BASE COUNT         2178 a         5516 c         5239 g         1979 t
ORIGIN      
        1 ggacctgcac ccgctggtcg acgtcatcgt cgtggcccgc ggcggcggct ccgtgcgcga
       61 cctcgcgacg ttcgacgacg agcgcgtatg ccgggcgatc cgcgcgctgt cgacccccgt
      121 cgtcaccgcg atcggccaca ccgacaacaa cccggtctgc aaccacgtca cccacgcggc
      181 gtgggtgccg cgccacgccg ccgagcgggt ggtgcccgac cggcgcgagc tgctgcgcga
      241 tgtggacgac gccgcggccg ccgcccgccg cgccgccggc gctcagcgca tgcggatcga
      301 ggaggtgcgg gcgcgcttcg ccgcgctgag cgggcgcagc gagcgggtgc gcgcgcggcg
      361 cgtcgagctc gccgcgcacg gcgacgtgct cgaccacgcg atgcgcggtt tcctggacaa
      421 gaccatgcgc tccctcgcga gcgcccggga ggccttcgcg cgcacgcccg cgcgcgtgcg
      481 cgaagagctt gcggtccacg gccagcacct ggtggacttc cgcgcggcgg cgcgcgtcgc
      541 cgcccgcgtc accgcgcagg cgcaccggga cgcatcgagc ttcgaggagg acctgcgcct
      601 gggcggccgg cgcgtgctcg gcgaccaccg ccgcaactac ggcaaggcgt tcgaccgcct
      661 ggcggagtcg accgcgaacg agacccgcaa gcggttcgtc cgcgagcgcg aggggttgga
      721 gcgcgtggga ggcgagctgg gcgtcgcggc ccggcgcggc ctgcgggtcg cgggcgaccg
      781 cgcttcgctg ttcgtcggcg agcagcgggc ggcgcagcgg agcgcggcgc ggcggcggct
      841 cgacgccagg cgcgagctcg tgcgcgagcg cgcggccacc acggcccgcg cgggacggcg
      901 cgtggccagg acgcaccgcg agaactacgg caaggcgctc gatcgccaag tggggacgat
      961 gcgccggtcg accgagcgcg cgctcgaggc cgagcgccgg cggctcgagc aggagcgcga
     1021 gcgccgtggc gacgcggcaa cgcgcgccac gcgcgacatc cgccgccgcc taggcgcggt
     1081 cgtcgacctg ctccacgcga gcgacttccg ccgccgcggg ctcctgcccg tcaccggcgc
     1141 gggtggccgc gtgatccgat cgatcgagga cgtcgtggtg ggccgggcgc tgaccctcca
     1201 cctgcacgac ggcgaggtag aagcgagtgc ggagcgggtg cgcccctatc ccgcgctccc
     1261 caacgactcc gaggacaacg gccgcgagga ggcccaatga gcaccgaggc agcaaaggcg
     1321 atcacgttca gcgacgcgta cacggagctg aagtcgatca cggacaccct gaacgcggat
     1381 caggtggagg tggacgcgct cgtcccgctc ctgcgcaggg gcaaggggct cgaggccgcg
     1441 ctgcgcgcgc acctggagca ggtcgagcag gaggtcgagg cgatcgaggc gggggagggc
     1501 atcaccccgt accgcatcgt cgcgccggaa gcggccgcgc cggccgccga gccggccgcg
     1561 cgctccctcg acgccgcgcc gccgaacggc tccgtggccg acgacatccc gttctagggg
     1621 tcggagggcg ccccatgcgg agcctaggcg gccgcacgca gcgctcctca ttcgaggtgt
     1681 tcgtcctgac tcacggtatg cgtgacttag aacgaacacc tcgcgtgcag tttttccgtc
     1741 taccccccgg cttaccttga gggtcgaccg caagcccgct atccccaggg agaccccacc
     1801 gcaaatgcca ctctgcttca tcgagccaca ggaagcgatc cgcgtggtgg cggagtcgat
     1861 cggccgcgag gcgcgccggc agctactcgg cgaggtggtc cgtgcgtggg tggacgagat
     1921 ccccgaggac gagctcgagg tccactacgc caaggcggtg gaggatctcg acgacgacga
     1981 cctctcgatg ctcgctgcct ggcacgcctc gctggaggtc ggccagccgg tggcgaacaa
     2041 ggacttcctg ctgaaggcat tctccagcct cggcgagcac cgccgcgagg cgctgaagat
     2101 cgcggcgggc ttccgcggcg acatcgcctt cgagtcgggc gtgagcgagg agcaggcgct
     2161 cgacacggtc ctgccctggc tctcggcggc ccgggcggag gagttggctg ccgatgcggt
     2221 tgagctgtac tgctgggacc gcatgggctc cgacaactag ggcggctgca aaccttcgcc
     2281 tggctgcgtc ctcggtcgct cgcgtgctag cgcacgcttc gctccctgcg tccttgccat
     2341 gctcggtttt cgccgccctc gcgctcccgg tgcggccccg gcgcgtctcg ctcccggcgg
     2401 ccctcgttca caggtccgcg ccccggcggg tctcgctccc cagcggctcg cgcacgcggc
     2461 tcgcgcacgc ggctcgcgca cgcggcccgc gcacgcgcca cgcgcacctc gcgttgcagc
     2521 ccgtccggtg actggcgcac cctgcttctc ccatgcttcg ccgccgctcg cgccgccgcc
     2581 gcctccggag ggctccggcc acactcgtcc tcctcctaat tgccgccgtt gccgtctcct
     2641 acggggaccg cgtcggcggt agcggagcca acggcggcgg cggcgcgccc ggctcgagcg
     2701 gaggctccgg ccccaccgcc cccaccggcg ccgagaccgc ccgcgtcgag cgcgtcgtcg
     2761 acggcgacac gatccgcctg cggggcgtcg ggccggtccg gctgatcggc gtcgacaccc
     2821 ccgagaccta cggcggccgc gacgagtgct tcgggcccga ggccacgcgc tacgtcgagg
     2881 gcctgcttcc gcgcggcgcg cgcgtgcgct acgtcgtcgg cgaggactcg cgcgaccgct
     2941 acgggcgcct gctcgcctac ctctacctgc gggatggcgc ctcgctcaac cacgcgctcg
     3001 tcgccgccgg ctacgcccgc accctcacga tcccgccgaa cgaccgctac gcgcccacct
     3061 tcgagcgggc cgaggaggga gcgcggcgcg cccgccacgg gatgtgggcg aggcccggct
     3121 gcgccggcga gtaggtcgcg ccatgaccgc gttgccgccg cgggtccgcg aggccgccgc
     3181 gggtccgcac cgccgccgcg gtccgcgagg ccgccgcgct gcggccgggc gccctacccc
     3241 ctcagcagct cctccacctc ggcgatcgcc tgccccgcat ccacgaagtg caccggcgtc
     3301 atgcccagct cgcgcgcggc gtcgcagttc acgtcgatgt cgtccacgaa cacgcactcg
     3361 gaggcggcca cgtcgccgag ccgctcgagc gtcagctcgt agatccgtgg gtccggcttg
     3421 cgcatcccca cgaaccccga gtcgaccacg atctcgaaga tctcgtcgac cgcggggatc
     3481 ttggctcgcc atagcggctc ccactcgcgc acgttgttcg tgagcagcgc catccggtag
     3541 ccacgcccgc gcagcccgcg catgtactcg accaccggct cgttcgggtg aaggtgagcg
     3601 aagtagcgct cgcggaagtc cgccaggctc actccgccgc cgagctcggc ctcgatcacc
     3661 gcgtagaact cctgctcggt gatccggccg cactcgagct cgaacagcgg atgcgccccg
     3721 tcgcgctcca tgatccgccc catcgcctcg ccgaagcggc ggaagtccac gcccgtctcg
     3781 tcctggtagg caacgaagga ctggagcagc ggggtagtga gcaccccgcc gaagtccgag
     3841 atgaccgcgc ggatcacgcg gcgaggtccg gctcggctcg cgccgtgatc tcgtaggagc
     3901 ccgcgccgtc gcgcccctcc atccgccagg cgaccacgcc ggcgtccacg cgcagctcct
     3961 cgagctcgag cgacagaccc gcccgcaccg agcccgacgc gcgccgcggg aagtcctcgc
     4021 caggcagcca cagctcgagc ccgaccgagc gctggcggcc gtgggcgtcg tagacggtcg
     4081 agagccgcgc ctcctcgacg ccgaacagct ctccgccggc gagcaggtgc gcgacgacca
     4141 gctcctgccc gtgcccgatc gccccccgcg ggcgccgcgc cgcgagcacc accgcgtgct
     4201 cgtcgtcgaa gacggccgac acgagccgga ccgcgtcgag ctccgcccac gccggcggcg
     4261 tgcgcgtctc gcccacggtc ccgaggcagt cgacgagctg accgcccgcg cgcccgtcca
     4321 cccggcacac acgcgcggag gcgccgccca gctccgcccg cggcgagaac ggccggaacg
     4381 ccagatccac ctcgtcgccg cagcgtgccc ggtacccgcc agccccgtcg ggagctatct
     4441 cgggcagctc ggcgagcgtc cggccgccgg acccctcgaa cagcacgccg accccggccc
     4501 gcaggatgcc gtgcacctcc tggcccgggt cgaagaaccc aagtgagatc gccggctcgg
     4561 tcacggctcg ccgggccccg agcgggggag ccggcgactg tctgtgtcga cctcggtggc
     4621 ggtgccctcg acgtcgtagt agccctcggg cacgccgttg cgcgcctcgc gcgccgcctg
     4681 gcgctcgcgc ccggcgcgca ccgccgcgcc gccgacgcgc ccccacagcg tcccgcgctc
     4741 gatccccccg cgcaccatgc ggcggaacag cgcgcgcgtg ggcggcagca ggaagaacag
     4801 ccccaggatg tccgtcagga acccgggcgt gatcaggaac gcccccccga agatgatcag
     4861 gacgccgtcg atgatctcgc tgtgcgggat ccgcccggcg gcggtcgcct cgttgaagcg
     4921 ccgccagacc tgccggccct gcgacttgag cagcatcgag ccgaggatcg agtcagcagc
     4981 gaggatcagc agcgtcggtt cccagccgag cgagtcgccc accttcagga tcacgtacag
     5041 ctccgccagc gggacgacga tgaagatgac gatcagcgcc aagaggggca caggccgagg
     5101 ctactaacat cgcaccatgc cctcagtcga cgaagtgacc gaagcgctga ccaacgtgat
     5161 cgaccccgag ctggggctcg atttcgtcga gctcggcctg gtctacgagg tcgaggtcga
     5221 cgattcggac gtgcacgtca cgttcacgct cacctctccc gggtgcccga tcgggcccca
     5281 ggtgtccgag cagatgaagg agttcgtggg ggagctcgac ggagtcgaga gcgtccaccc
     5341 ccacatgacc ttcaaccccc cgtggacacc ggacctgatg agcgaggacg cgaagttcgc
     5401 cctcggctac tgagctccgc cgcgtcgagc agctccgcga gcgactcgcg cacggcgtcg
     5461 gcgaagaagt cgagatccgg catcgcgtcg tagtcgccca gcaggccgaa gtcgagcgtg
     5521 ccgttgtagc tcatgatcgc caccgccatc gcgtggttct ccggcaggaa cgcgagcggc
     5581 acgatctcct gtagctcgcg accgagcagg tagagcggga actgcggccc cggcacgttc
     5641 gtcacgatcg cgttgaagag gcgcgtcgag aagttcagcc gcgaggcctg cgcgaggatc
     5701 gtcggcggcg cgaagtcgtt cagcccggcg atcacctcgg cgccgagcgc ctgcttggac
     5761 tgcttgagcc cctccatcgc ctcgcgcacg atcgccagcc gctcgaccgg gtcgtccacg
     5821 tacacgggca gcggcccccg catcgcggcg atccggttac cgagctggcc ctgctcgtcc
     5881 tcggcacgga tcgacaccgg cacgagcgcg cgcagctcga gcccctcggt ccgcaccccg
     5941 cgcgagcgca gccagcgccg cagcgcgccc gagacgaccg tgagcaccac gtcgttcacg
     6001 gtcccgccta gctcgttctt gacgcgcttg aggtcgccga gctcggcgcg cacgcggtcg
     6061 atgcggcggt gcggcccgat cggcacgttg agcgggatcg acggcgccgg gttgactccg
     6121 agccacgcca cctcgccgag cgcctggacc gcctcgcgcg cctgcgcgag ggtgcgcccc
     6181 ggcgactgca ccgcccgcag cgcccgctgc gccgcgccga tcggcgcctt tgccacgtcc
     6241 ttcacgccct ctgcgatcag ctcggcgtcc gagggcaccg gccgcggcgt ccacggctcg
     6301 ggatccacct tgcgcggcac cgggtcgatg tcgaagagca cgctggcgag gtccacgccc
     6361 gcgatcccgt cgatcatcga gtgatgcgtc ttggtgatca gggcgaagcg gttgtcgtcc
     6421 atgccctgga tcagccacgt ctcccacagc ggcttcgagc ggtccagccg ctgcgagtag
     6481 atccgcccgg cgagcttcag gagctggtcc accgacccgg gggtcggcag cgccgtgtgg
     6541 cggacgtggt actcgaggtt gaacgtgggg tcgtccaccc acatcggccg gcccatctcg
     6601 aagcgcggga acgcgagctt ctggcggtag cgcggcgtca ggtgcatccg cgaccggatg
     6661 ccctcgagga agtccgtgta gtccggcggc ggcccctcga agagcaggat cgcgccgacg
     6721 tgcatgtgcg acgagcgctt ctcctgcacc aggaacgacg cgtccaccga ggtgaggcga
     6781 tccttgtggc cgggcatggc gggagcgtat ccgctggcgg tgcgcgccac aatggagcca
     6841 tgaccgcccg gaccctgacc gccgccgcgc tcgccgccgc cgcgctcgcg ctcggatgcg
     6901 gcggcgacga cgaggtcgag gcgccggccg tggagcccgc ggccagcgtc gacgaggggc
     6961 gcttcgacgg cgccgccgcc atgcgctggg tgcgccgcca gctcgcctac ggcccgcgcc
     7021 cggctgggtc gcccccctcg gtcaagctcg ccaagcggct gcgcgacgcg ctcccgaacg
     7081 gccgctacca ggcggtgccc ggcggcctgc gcaacgtgct cggcaccgtc cccggccgcg
     7141 acccgaagcg gctcgtggtc gtgggcgcgc actacgacac gaaggacatt ccgggcttcg
     7201 tcggcgcggt cgacggcgcc tccggcaccg ccgtcgtcac ccagctcgcg cgcacgatca
     7261 agccgcgcag cgcgcgcccg acgatcgtct tcgcgctctt cgacggcgag gaggctccgg
     7321 ccggccgccc cgacgccgag ttcgagcgct acggcatccg cgggagcaag gtcgccgcgc
     7381 gcgccctgcg ccgcgccgag gcgatgatcc tgctcgactt cgtgggggag aaggacctcc
     7441 agctcccgcg cgaggccaac tccgaccccg cgctgtggcg caagctccgc gccggcgccg
     7501 ccacggcggg ggtgatcgac cgcttcccga accgcaccgc gccgcccgtc tcggacgacc
     7561 acaccccgtt catccgccgc gggatcccgt cgatcgacct gatcgatttc gagttcgagt
     7621 gctggcacga gacctgcgac gacctgtcgc agatcggcga ggagagcctc gacgcgacgg
     7681 gtgaggcgat ggtccgcatg ctcgctacgc tctgaggcga tgccgcacgc gcccgagagg
     7741 cttctcctgg cggctccgcg cgggtactgc gccggggtcg atcgcgcggt ccaggcggtc
     7801 gagcgcgcgc tcgagctcta cggcccgccg gtctacgtcc gcaaggagat cgtccacaac
     7861 aagcacgtcg tcgagcagct cgccgcgcgc ggcgcggtgt tcgtcgacga ggagaccgag
     7921 gtgcccaagg gcgagacggt tgtcttctcc gcccacggcg tcgcgccgtc ggtgcacgcg
     7981 aacgcgctcg agcgctcgct gcgcacgatc gacgccacct gcccgctcgt caccaaggtc
     8041 cacgtcgagg ccaagaagtt cgccgccgac ggctacacga tcgtcctgat cggccacgag
     8101 ggccatgagg aggtcgaggg cacgatgggc gaggcgcccg agcacatcgt gctcgtggag
     8161 agcgaggccg acgtcgagac gctcgaggtc gccgatcccg agcgcgtcgc cttcatctcg
     8221 cagaccacgc tttcggtcga cgagaccacc gccatcatcg cccggctgcg cgagcgcttc
     8281 ccggccatcg tcggcccccg caccgacgac atctgctacg ccaccaccaa ccgccagatg
     8341 gccgttcgcc agatggcccg cgagtgcgac ctagtgctcg tgatcggctc gcggaactcc
     8401 tcgaactcga accggctggt cgaggtcgcg cgcgcgttcg gcgccgactc ctacctgatc
     8461 gacaacgagc acgaggtgcg cgaggagtgg ctcgagggca agcgcacggt ggggatcacc
     8521 tccggcgcca gcgcgcccga ggagctggtc cagcggctcg tcgacttctt cagggagcgg
     8581 ggcaccgagg acgtctcgga gttccaggtc gtccaggagg acgtccgctt catgctcccg
     8641 aaggagatcc gccgcgagct cgcggcgcgc gcgtagggcg aggccctact ccggcgggac
     8701 cggcgtgaac tggaacccgt gcagtcgcag gcccgcctcc tcggcgacca ccgtgccgtc
     8761 ggtctcgtag agcccgggct cgagcccgcc ctgcgatagc accgcccacg cgctgcccgc
     8821 ctcgaagcgc acgctcgccg acgcacccgc gtcggccgcg gcgatccagt cctcgccgtc
     8881 ggtccagtcg cgcacgagct ggagccgctc gcgctcggcc ggcagcgtca cgtccgcggt
     8941 ctgcggcgtc atcaggacac ccggggcgtc ctcgggccgg aacggctcga gcggcgcggg
     9001 cagctcggcg tccggctcga tctcgcgcag cacctcctgc accgccagct cggttccctc
     9061 gtagtcgccc tcgccgtagt ggaagtagcg caggatcccg cgccggtcga acacgtagcg
     9121 gcccggccag ccgcggttgc cgtactggcg ccagacggcg tagtcggggt cgagcgcgac
     9181 ggcgaacggg atgccgagcc gctcgatcgc ggcgctcacg acgtccgggt cgcgcccgaa
     9241 cgagtagccg ggcgagtgga cggcgatcac gcgcaggccg gcgtcggcgt agcgctcgtg
     9301 ccaggcgacg aggtagggca gcgtgcgcag cgagttgatc cgcgcgaagt cccagaactc
     9361 cacgagcacg ccgtggcggc cgagcagctt gtccatccgc aggaacgcga cgttgcgcca
     9421 ctcgatcccc ttcgggaact cgggcgcgta caggtcctcg tcgtgcggcg gcctcacggg
     9481 ccgcgaggct atccgccgcg ggcggttaga gcccggcgat cgagcggatg acgtccggcg
     9541 tcgcgtagac ctcgaccatg caggcgatga agagcaccgg gatggcgagg ccgaccgtga
     9601 cgacggtcgc ggccagcagc ttgtgccagt cgccgcggcg gctcgccacg acccaggcgg
     9661 cgagcggcag gaacagcgcc gtcagctccg ggatcgcgtg gagcgagagc gccagcagca
     9721 gcttcagggg gctgatgccg aggttcgcgg cgaccgtgga cgtcgcgctg ccgatcacgt
     9781 aggcctgcgt gcagagcgag aacgtggtgg cggcgatcac gaaggcgatc gcgaacgggc
     9841 ctgccttctc gtgcacccac ttgttgacgc ccgtcttgta ctgcgcctgc tgcggcaccg
     9901 cgctgccggc gaggaagccc gcgacgcacg ccatcccgtg cagcgcgagc acgagcgagt
     9961 tcttcagcag cacgtgctcg gcgtcggcga aggtcgcctc gcgctgcacg ccggggatga
    10021 tcagcgacgt ggggtcgggg gtcgacaggc gcgcgacggc gtagaccgcg accagcagac
    10081 cgaccgcggt cagcagcgac cagccgagcc agccccgcaa cgttccccac ggatcgctgt
    10141 tccattcggc gagggtggcc ttcgtcgcgg ccatgccctg cacgaggacc agttcgttcg
    10201 agttcaccgg ttgaccatcg gcatgccgcc gcatgtccgt gagcatgcca ccgaatcgtt
    10261 tggcgagccc ggtgttctgg ctctagagtt ggactcgtcc ccctgcatca aaggagagat
    10321 agtgcatcca ttcaagactt cgcgcctagg cctgctggcc gcggccacgg cgctcatcgc
    10381 cgtgctcgtg gcggcgccgg gcgcgggcgc cgccaccttc gagggcggcc cgtggaagat
    10441 cgacttcaag acgttcacga aggcgaagaa gctcaagctc acgaccacgg gtgcgaaccc
    10501 caagtcgtcc accggcggca ccttcgggct cggcaccggc cagatcacca acaacgagca
    10561 ggccagcggc aacatcagcg ttggcagcag cacgtcggtg atccagttcg cgctcggcaa
    10621 gaagaaggtc accctgacct cgctcaccca gaagctgacc gccggcaagg gtcagatcaa
    10681 cgcggtggtc aacaagaagg gcaaggcggt cacgctcttc gacgtcgcga gccagggcaa
    10741 ggtcggcccg aacagcacgt tcaccaagct gacgctgaac tcgtcgaacg tctcgctcac
    10801 caagtccggc gccagcgcgc tgaacaaggc cttcggcttc aagggcacgg ccgtcctgaa
    10861 ggccaagcag aaggtcggca cggtcgggtt caccggggag cgctcgctca cggtgctcag
    10921 cggcacgtcc acgtcgctct acgacaaggc cttcggcgac gcgctgaaga gctgtgacat
    10981 cacgctgagc gcggtgcagc ccgcgtccgg catccccgcg gatgccaaca acccgtacgg
    11041 gggcgtcaac ctccccgtca acggtgccaa cggcggcacc ctcgacgcgg agacgctgac
    11101 cggccaggtc aaccacgagg gcggcacctc gctcgaccgt ccgggtcccg gacagccggg
    11161 gaacacgacg ggcaaggcgg agtaccactc gccgctggtc aacttcgtgt tcggcttcag
    11221 cccgggctcg ttcacgctga cggcgttcgt gacgaactcg aacaacaacc tgccgatcgg
    11281 ctcggtgacc ggctcgctcg acaagaacct gaccgctgag gcgggcacgg tgacgctgtc
    11341 gaacggcgcc ctcaacctgt cggacgcggc ggcgggcacg ctgtcgcagg cgcagccccc
    11401 gctcggggcc aactgccaga tcccggcggg ctcgaagatc gggtcgctca actcgacgtt
    11461 caacgtcggc tagcgaccga gcagacacgc agtctgggaa cggccccgct ccggcggggc
    11521 cgtctcgttc tggggctagt tcgcgccgag ctcgcgcagg tcgtgcgcga ggcgctcggg
    11581 cgtcgcctcg ctggcgggga agccgacgcg ctggaagccg tcgccgtcca ccagcacgat
    11641 ccgcgcctgg tgctcgtgct cggggagctg ctcctggatc gcgaacccgc gccagagctt
    11701 ctcgagctgg gtccgcgagc cgagcaccca gcgcatccgg ccggcgaggt cgtacttggc
    11761 gaggaactcg cgcgcgcgtg cgggcgtgtc gcgcggcggg tcgacggcga tcgccagcgc
    11821 cggcaccggc cggtcgagca ggtcgagcgc gcccttgatc tgctgcgcct ccgtcgggca
    11881 ggtgtcctcg caggtcgtgt acaggaacgt gaccgccacg gggcgcccgc gcagctcccg
    11941 catcgacacg cgccggccgc gctcgtcggt cagcgcgaag tccggcgcgc gcacgccggg
    12001 cggcatggtg ccgccctcga agcgggtcgc gggcgaatcc tcggagccgg cgttcgcccg
    12061 gtcgcgggcc cggtccacga ccacgagcag cgccacgaac gccaccgccg tggtcaccat
    12121 caccgcaatc gccgtccggg gccgcattgc tcctgatggt gacaaatggg gcctagaccc
    12181 catgcggggg tccgcccggg ggccgatgat gggggtgtaa ggcgatcagc acacgggtcg
    12241 ctctccggtt tccaaatact ccttgggtct gtggtcgggg cgtcgcgaaa gcggcgcccc
    12301 gagtgctttc cggggtcccc gacgggcctc gacagggtcg ctacactttt tgaagtgaaa
    12361 gccgaccagg attaggggag ttcgagaccg acatgccgac cggcgctgaa gtcctcagga
    12421 agatcaagca ggagatcagg gaggtggacc cccgcgaggt ccacgacgcc accgcgaacg
    12481 ggaacggcag cgcgcccgtc ctgatcgacg tgcgcgagca gcacgagttc gaggagggtc
    12541 acatcccggg cgccgtccac gtcccgcgcg gccacctcga gacgcgcatc gaggggcgcg
    12601 ccggcgaccg ctcccagccg atcgtcctct actgcgcctc cggcaaccgc tccgccttcg
    12661 cggcaaagac gctccaggac ctgctcggct acgaggacgt cgcgtcgatg accggcggca
    12721 tcacgctctg gaaggaccgc gggttcgagg tcgagacccc gcgcgcgctc acggccgagc
    12781 agaagacgcg ctactcgcgc cacatcctcc tcccggaggt cgggctcgag ggccagctcg
    12841 cgctgctcga cgcgaaggtg ctgctgctcg gcgccggcgg cctcggcgcg cccacggcgc
    12901 tctacctcgc cgcggcgggc gtcggcacgc tggggatcgt ggacgacgac gaggtcgacc
    12961 tctcgaacct ccagcgccag gtcatccaca cgaccgaccg gataggcgtc tcgaaggtcg
    13021 actcggccga gcagacgatc gccgggctga accccgacgt cgacgtggtc aagtaccaca
    13081 cgcgcctcga cgcctcgaac atcatggaga tcatcgacgg ctacgacgtg atcgtcgacg
    13141 gcgtcgacaa cttcccgacg cgctacctgc tgaacgacgc gacggtgcgc acgaagacgc
    13201 cggtcgtctc cgcgtcgatc ctcggattcg acggccagct ctcggtgttc gccccctacg
    13261 aggggccgtg ctaccgctgc ctctacccga cgccgccgcc ggccgagctg gctccgtcgt
    13321 gcggcgccaa cggcgtgctc ggcgtgctgc cggggacgat ggggatgctc caggccaccg
    13381 aggtcatcaa gctgatcacg ggccagggcg agccgctgat cgggcgcctg ctcctgtacg
    13441 aggcactcgg cgcgtcgttc accgagctca aggtccggcg cgacccggag tgcccgatct
    13501 gctcgcgcga gccggagtcg atctccgacg acgagatggg cgtcttcccg gactacgaag
    13561 cgttctgcgc gggcgctggc tagcctcccc ggatgagcaa cgtgaagatc ccgccggtcc
    13621 tccgctcctc cacgggcggg gagaaggagg tcagcgccgc gggcgggacc gtcggtgagg
    13681 tgctgcgcgc actcgccgag gcgcatcccg acaccgagtc gcagatcttc tccggcgacg
    13741 gccagctcaa ccgctacgtc aacgtctacc tcaacgacga ggacgtgcgc gtgctcgacg
    13801 ggctcgagac acccgtgaag gacgccgaca cgctggtgat ccttccggcg atggccgggg
    13861 gatagcggct cctctccgta tcgaggtcga ggggcgctca ggcgttcccg tcttctggtt
    13921 cgacgcgccg gggcccgtcg aggcgcggct cgtgttcggc gtgggctcgg cgcacgagtc
    13981 gctgccgcgg cacgggatca cccacctcgt ggagcacctc gcgatggtgc cgctgcactg
    14041 gacgccgtac gcctcgggcg cctgggtcga ggacaccacc accacgttct gggtggaggg
    14101 cagcctcgcg gaggtcggcg gcttcctggg cgacgtcacg cgctcgctgg gagagttgcc
    14161 gctcgatcgg ctggacgtcg agcgcgacgt gctcgcgagc gaggccgccg attcggggtc
    14221 gagcttccag cgccggctcc tgggtctgcg cttcggcccc gcgacctacg gcgtggccaa
    14281 cttcctggag ctcggcctgc ggtccgtgtc gcccgaggag gtggaggact ggcggcggcg
    14341 ctctttcacc gcgggcaacg ccgcgatctg gatgacggca cgcccgccgg acgacctggt
    14401 gctcgaccta ccggacggcc ccgcggtgcg gcctccggag cccgagccga tcccgggact
    14461 gcgcctcccc gcgcagagcg ctgactggga gggcgacgtc gcgttcggca tgctcgcgtc
    14521 gcgcagccac gcactcgccg cgggcggaat gatcgccgag cgccgcgccg aggatcgtct
    14581 gcgcgtcgag cgcggcctca cctacggggt cgagtgggag ctcttcccgc tcaccgccgg
    14641 caccgcccac ctgttcctct ccgccgactc gaccgacgcg aacgccgtcg tgatccgcga
    14701 cgggatcggg gcggtgctcg aggagctcgc gcgggacggg cccaccgagg aggagctgga
    14761 acggttggtg gccgaggacg agcgctggat gcacgacccc ggcttccccg gcgagatcct
    14821 cgaccgggcg gcgcgcgagc atctccgcgg ggcgccgctg gagcagcccg gggagctgat
    14881 ggaggagcgc cggcgggtgc gccccgccgc gg
//
LOCUS       DASVPL010000010         3979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_157267, whole genome shotgun sequence.
ACCESSION   DASVPL010000010 DASVPL010000000
VERSION     DASVPL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157267"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="VF517_00515"
     CDS             <1..1599
                     /locus_tag="VF517_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8051445.1"
                     /translation="RWGERALERLLGDWALAAWHPRERRLVLARDHYGPTALYYHRRG
                     DRLAFASSLKGLLALPDVPRRLDELQLARALVMDVADGAATMYEGIRRLPTARAVSFD
                     ASGVRTREYWSLMDVPEVRLASDGEYVEHLLDLLGAAVHTRLRSRAPLATTLSAGLDS
                     SAVTALAARELDGAPLTAYTVRPAYPEVAAEMPDVLVDEWPGAQLVAASYPNIEHVPV
                     DARTVSPVEAIEHSLAVHDEPEHAVPNLPWVRGLLDAAREGGARVLLTGQYGNGGVSW
                     PGDARGALNALAGGEPRSAAQRLRHLSRASRYGWGGAVWHGLAMPARSIVAAERMRRD
                     PTRRPGWRSSAIAPHFAARIGLRDAVRENGWDPEFTRAGPRERRLSYLLPGRLPTGAW
                     WHQRSAAQGVEMRDPTADVRVLEFCVGAPDEQFAGVGGIDRWLIRRALALLVPPEVAW
                     STRRGAQGADIAYRLRADAGAVSAAVERVVSSDAARDHLDVVALRRSWAEVAAGSSDG
                     VLGVTRALAFGLFLAGLGDDDGGL"
     gene            1612..1788
                     /locus_tag="VF517_00520"
     CDS             1612..1788
                     /locus_tag="VF517_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051446.1"
                     /translation="MSEHSNPHEPQRAATSSESGAGAKAPWHPPTLEEVDYSATEAAG
                     VGAVYDLTVYTGSV"
     gene            1910..2923
                     /locus_tag="VF517_00525"
     CDS             1910..2923
                     /locus_tag="VF517_00525"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEX8051447.1"
                     /translation="MAAEIVEGLGVPTTSEALRARVSERFDVGPEECERAVGAFVDEL
                     AALGVVETRPAAEAPPPLRRRYLDLLKLALVNLVYAEDALRLDTALSGKLGPDQLARQ
                     RMLRDVRYERPDDYAEVVANKRDGAVDGFWGARLSHTMIGLSGLENLERCAHRVFADG
                     VPGDFLEAGVCHGGASIFLRGLQVAYGEDDRVTWLADTFAGVPPPAHEVDREHDLDLT
                     EERIPWMVAGIQEVRDNFETYDLLSDRVRFLPGLFADTLPDAPVERLAILRIDGDLYE
                     STRDTLDALYDRVSDGGYVIVDDYHCLDPCRIAVDEFLAKRGLDVEMDQVDWTRVCWR
                     KGA"
     gene            2920..>3979
                     /locus_tag="VF517_00530"
     CDS             2920..>3979
                     /locus_tag="VF517_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051448.1"
                     /translation="MSGPGDPRLVVVYPRDLIGENAGLYGLHVTSLVELLTPLGWRVE
                     AYTVLDPRFPAAALGADLTVVQMLADPEVEGVILCRRDRGLPTVYEVTDNVLGVGDWL
                     PRTHPARSPLARQSLLYHAHLADSTQMLVPALAELFSGVNPRSILLSPRTPFPDEVPP
                     KPPGFVFGWSGSRSHSASLAAAAPAVIELCHRHPDATFAFMGDRSVFDEHFGAIRAEQ
                     RQVHPFGEWEAQLRFVGGLHVGIAPMAPSPFNAARSDTRVGLYAGHGVAAVLEDAPPH
                     RPHRDRARIYRTAAELLDVLEELFHDRPQVEAMARNGRAWMERERSAAALGAQRDGAY
                     RELLARSPNGRPGGPPDSP"
BASE COUNT          495 a         1466 c         1464 g          554 t
ORIGIN      
        1 cgctggggcg agcgggcgct cgagcgcctc ctgggcgact gggctctcgc ggcctggcat
       61 ccgcgcgagc gccggctggt cctggcccgc gaccactacg gcccgaccgc gctgtactac
      121 caccggcgcg gtgaccggct cgccttcgcg tcgagcctga agggcctgct cgcgctaccc
      181 gacgtgccgc gccggctcga cgagctccag ctcgcgcggg cgctcgtgat ggacgtcgcc
      241 gacggggcgg cgaccatgta cgaggggatc cggcgcctgc cgaccgcccg tgccgtcagc
      301 ttcgacgcga gcggcgttcg cacgcgcgag tactggagcc tgatggacgt accggaggtg
      361 cggctcgcga gcgacggcga gtacgtcgag cacctgctcg acctgctcgg agccgccgtg
      421 cacacgcgcc tgcgctcgcg cgcgccgctc gccacgacgc tcagcgcggg gctcgactcg
      481 agcgccgtca ccgccctcgc cgcccgcgag ctggacggcg cgcctctcac cgcgtacacg
      541 gtgcggcccg cctacccgga ggtggccgcc gagatgccgg acgtgctggt ggacgaatgg
      601 cccggcgcgc agctcgtcgc cgcgtcctac ccgaacatcg agcacgtgcc cgtcgacgct
      661 cgcaccgtga gcccggtgga ggcgatcgag cactcgctcg cggtgcacga cgagccggag
      721 cacgcggtac cgaacctccc ctgggtgcgc gggctgctcg acgcggcgcg cgagggcggc
      781 gcgcgcgtcc tcctgaccgg ccaatacggc aacggcggcg tgtcgtggcc gggcgacgcg
      841 cgtggggccc tcaatgccct cgcgggcggc gagccgcgct cggcggcgca gcgcctccgg
      901 cacctgagcc gcgccagccg ctacggctgg ggcggcgcgg tctggcacgg actggccatg
      961 ccggctcgga gcatcgtcgc ggccgagcgg atgcggcgcg acccgacccg ccggcctggc
     1021 tggcgcagtt cggcgatcgc gcctcacttc gccgcacgga tcggactgcg cgacgcggtg
     1081 cgcgagaacg gctgggaccc cgagttcacg cgcgccggcc cgcgcgagcg ccgcctgtcc
     1141 tatctcctac ccggcaggct gccgaccggc gcgtggtggc atcagcgcag cgccgctcag
     1201 ggggtcgaga tgcgcgatcc gaccgcggac gttcgcgtgc tcgagttctg cgtgggtgcg
     1261 ccggacgagc agttcgccgg cgtcggcggc atcgaccgct ggctcatccg gagggcgctc
     1321 gcgctgctcg tgccgcccga ggtcgcctgg agcactcggc gcggagcgca gggggccgac
     1381 atcgcgtacc ggctgcgggc cgacgccggc gccgtctcgg cggcggtgga gcgtgtcgtc
     1441 tcgtccgatg cggcgcgcga ccacctcgat gtcgtggcgc tccggcgctc gtgggccgag
     1501 gtcgccgcgg gcagctcgga cggcgtgctg ggcgtcacgc gcgctctggc gttcgggctc
     1561 ttcctggccg gtctcggcga cgacgacggg ggcctgtgat tcactatgcc gatgagcgag
     1621 cactcgaacc cccacgagcc gcagcgagcg gcaacgtcct ctgaatccgg cgctggcgcc
     1681 aaggcgccat ggcacccccc gacgctggag gaggtcgact actcggccac cgaggcggcc
     1741 ggcgtgggcg ccgtgtacga cctcacggtc tacaccggca gcgtctagcg gcagcaccgc
     1801 gccgcatttg aacacgctcc tccggtcgga gcgggcgttc gtacgccggc gcggcggcga
     1861 gtcgttcctg gtccggcccg cccagcgggc tgagtcgagg ctgagcgagg tggcggcgga
     1921 gatagtggag ggccttgggg tccccacgac ctccgaagcg ctccgcgcgc gcgtgtcgga
     1981 gcggttcgac gtcggccccg aggaatgcga gcgggcggtc ggcgcgttcg tcgacgagct
     2041 ggcggcgctc ggcgtcgtcg agacccggcc cgcggcggag gccccgccgc cgctgcgccg
     2101 gcgctacctc gacctgctca agctcgcgct cgtgaacctc gtgtacgcgg aggatgccct
     2161 gaggctcgac accgccctgt ccggcaagct cggcccggac cagctggccc ggcagcgcat
     2221 gctgcgcgac gtgcgctacg aacgccccga cgactacgcg gaggtggtcg ccaacaagcg
     2281 cgacggggcg gtggacgggt tctggggagc ccgcctctct cacacgatga tcggcctcag
     2341 cgggctcgag aacctcgagc ggtgcgccca cagggtcttc gccgacgggg tcccgggcga
     2401 cttcctcgag gccggcgtct gccacggcgg ggcgtcgatc tttctgcgtg gcctccaggt
     2461 cgcgtacggg gaggacgatc gcgtgacgtg gctcgccgac acgttcgccg gagtcccacc
     2521 gccggcgcac gaggtggacc gcgagcacga tctcgacctg accgaggagc gcatcccgtg
     2581 gatggtcgct gggattcagg aggtgcgcga caacttcgag acctacgacc tgctctcgga
     2641 tcgagtccgc ttccttccgg ggctgttcgc ggacacgctg ccggacgcgc ccgtcgagcg
     2701 gctcgccatc ctgcgcatcg acggcgacct ctacgaatcg acgcgcgaca cgctcgacgc
     2761 cctgtacgac cgcgtgtccg acggcggcta cgtcatcgtg gacgactacc actgcctcga
     2821 cccgtgccgc atcgcggtcg acgagttcct ggcgaagcgc gggctggacg tggagatgga
     2881 ccaggtcgac tggacccgcg tctgctggcg caagggcgcg tgagcgggcc gggcgacccg
     2941 cgcctcgtcg tcgtataccc ccgcgacctc atcggcgaga acgccgggct ctacggcctg
     3001 cacgtgacct cgctcgtcga gctgctgacg ccgctcggct ggcgcgtcga ggcgtacacg
     3061 gtcctggacc cccgctttcc ggcggcggca ctcggcgccg acctcacggt cgtccagatg
     3121 ctcgccgacc ccgaggtgga gggcgtgatc ctgtgccgtc gcgacagggg ccttccaacc
     3181 gtgtacgagg tgaccgacaa cgtcctcggg gtcggcgact ggctgccgcg cacgcatccc
     3241 gcgcgcagtc cgctcgcccg gcagagcctt ctgtaccacg cacatctggc cgactcgact
     3301 cagatgctgg tgccggcgct cgcggagctc ttctccgggg tgaatccgcg gtcgatcctg
     3361 ctcagccctc gcacgccgtt tcccgacgag gtgcccccga agccgcccgg cttcgtgttc
     3421 ggctggtcgg gcagccgctc gcactcggcc agtctcgcgg cggcggcccc cgcggtgatc
     3481 gagctctgcc accgtcatcc ggacgcgacc ttcgcgttca tgggggatcg gagcgtcttc
     3541 gacgagcact tcggggcgat ccgggccgag cagcggcagg tccatccgtt cggcgagtgg
     3601 gaggcgcagc tgcgctttgt cggcggcctg cacgtcggca tcgccccgat ggcgccgagc
     3661 cccttcaacg cggcgcggtc cgacacgcgc gtcggcctct acgcggggca cggggtggcc
     3721 gcggtgctgg aggacgcccc tccacatcgc ccccatcgag accgcgcacg gatctaccgg
     3781 acggcggcgg agctgctcga cgtgctggag gagctctttc acgaccgccc gcaggtcgag
     3841 gccatggccc gcaacgggcg cgcctggatg gaacgcgagc gcagcgccgc cgcgttgggg
     3901 gcccagcgag acggcgccta ccgcgagctg ctcgcgcggt cgccgaatgg acggccgggc
     3961 gggcctcccg attcgccga
//
LOCUS       DASVPL010000011         5768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_990, whole genome shotgun sequence.
ACCESSION   DASVPL010000011 DASVPL010000000
VERSION     DASVPL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VF517_00535"
     CDS             complement(<1..817)
                     /locus_tag="VF517_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX8051449.1"
                     /translation="MEALSPERARYDVDDRFYDEAFAARGEPHPHYRAAIEALEQRDL
                     RVLESASADAVRERGAYFGDNDAFAVDPVPRVLSAEEWSGLAAGLTQRVRALNAFLAD
                     IYGERRIVAAGVLPARVVESADHYEPWMAGVAVRGPRIAAAGLDVVRDASGRFMVLED
                     NVRTPSGIAYAEVAREVVGELLDRPGGDDFGPSFEALGAALRAAAPAGIDDPNVVLLS
                     DGESNSAWFEHRRIARRLGIPIVTFADLDIRDGRVFARHEGRREPVDVIYRRTD"
     gene            complement(827..2287)
                     /locus_tag="VF517_00540"
     CDS             complement(827..2287)
                     /locus_tag="VF517_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8051450.1"
                     /translation="MPSRLSTLDSSFLRVESPSAHMHVGWFSALELPRGVARLDAAAL
                     IDQIESRLHLAPRFRQRVVETPLGVGEPAWRDDPAFDMRDHVRVMEGPPLSAPAMRRA
                     VDAFLSEQLPRDRPLWSLLVLPAVDRRRAAFVGKVHHAMVDGLAAVELGLLLFDAGPE
                     ATPVKREEWRPASDNGPGRVLLDSLADSAVEQFRAARRVAALGLSPGSGLRMADTLRR
                     AAVSLAEDAIKPAPKSALNVPIGPRRTLVKHRVSMGRALRIKQQTGASLNDVVLTIAA
                     GALRKLALRTGDEPADLRVMVPVSVRRPDDERGEGNRITFAFVDLPVSEPAPLARLER
                     VREQMGELKESGRVAGTEILLRSVGSLPEPLKRRAAKLAASPRLYNLTVSNVPGPRIP
                     LYAAGARVRSIYPVIPIPEDHALSFGVLSYDGGLHFAAYADPEALPDAGRLPAMLEEA
                     VEELAVAVRPPGERVPAPRRRRGSHSRASLSSGRAG"
     gene            complement(2375..4102)
                     /locus_tag="VF517_00545"
     CDS             complement(2375..4102)
                     /locus_tag="VF517_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8051451.1"
                     /translation="MAAPDTCRVCAGALVLRHPGSAAAPDAESFSPSCHTPGAHGDLY
                     ACAECGTVQQPSLPPAAELAGLYREMSDEAYLAEEPGRRATARRLLDLIGEHVPAGRL
                     LDVGCGHGLLLDEARSRGYEVEGVELSHSAASYARDVLGLPVAETPLEEVEPADEGFA
                     AIVLADVLEHVEDPVAAIRRCRELLRPGGVLCVVTPDPGSRTARLAGARWWALLPAHV
                     CLVPRLTLRELLTAEGLVISDDVSFVRTFSARYWLAGLAERGGTLGTAVRAAARALPE
                     SASLSLSLGDERVVLAHNVEVLRPPKPLVAPRGREQDVHVVLPAYRAEPTIAQVAEEM
                     PVGAADRALLVDDASPDETVTAALRAGFEVIAHPANRGYGANQKTCYTRAVRDGADVV
                     VMVHADNQYDPALLAEMVRPIEDGEADVVMGSRLLEDETIAGGMPRWKWVGNRFLTWV
                     ENQAFRRDYSEYHTGYRAFSADFLRAVPFGRNSDGFVFDQEMFAQIVSRDARVVEQPI
                     PTRYFLEASSVSFRDSVAYGLRTLVVLARFRMHERGRTWPLLTQPAARLDDPPRGERR
                     FERGERTPV"
     gene            complement(4133..4564)
                     /locus_tag="VF517_00550"
     CDS             complement(4133..4564)
                     /locus_tag="VF517_00550"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8051452.1"
                     /translation="MLRSLVRAALVLACLVGILVSATVYRSEGRIESSVRTVVAGGAD
                     NAILAELAVARTKLNPDSLRDNSTAIVLARTGRADQAERLMLDVVKREPENQLVWVTL
                     ARVQVTAGHDDAARRSYRRAVALNSQTPPADLPPPLVRGKP"
     gene            4593..>5768
                     /locus_tag="VF517_00555"
     CDS             4593..>5768
                     /locus_tag="VF517_00555"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8051453.1"
                     /translation="MIALPVVLAFATGGYGLRRASFALAAVLLVLGALAVLAPWPLVP
                     RGKPALALAALAGFAAWTAVSIAWARVLGDAAADAYRLLLYVAAFMVALCCLRLPELR
                     RRVPDALLAGIVLVGLYGLAGRLLPAVVEVPEGARQDRLNEPFTYWNALGIFMVMGIV
                     LAVATASDEERPPWLRALACAAAAPCGVALYLTYSRASWLALAAGLVLLVVLRRTRAV
                     PLAAALALGSIAVLVAAVQAFPAVLGDEGTSTPAGLAFAGVIGVVGAAAALAHLRLSR
                     ARVAQAPLPIGPGLARVLAIVTIPAVIGVGLLVATGGGKNENVRSGGGRVATLDTNRD
                     ALWGVALDAFAEHPLTGVGTSSFAVEWLRERDEVDRALDAHSLYVETLAELGIVGALL
                     "
BASE COUNT          721 a         2140 c         2155 g          752 t
ORIGIN      
        1 cgtcggtgcg gcggtagatc acgtcgacgg gctcgcggcg gccctcgtgc cgagcgaaca
       61 cgcggccgtc gcggatgtcg aggtcggcga aggtgacgat cgggatcccg agccgccgcg
      121 cgatccggcg gtgctcgaac cacgcgctgt tggactcgcc gtcgctcagc agcacgacgt
      181 tcgggtcgtc gatgcccgcg ggcgccgccg cgcgcagggc cgctccgagc gcctcgaagg
      241 acggcccgaa gtcgtcgccg cccgggcgat ccagcagctc gccaaccacc tcgcgcgcca
      301 cctccgcata ggcgatcccc gacggcgtgc ggacgttgtc ctccagcacc atgaagcgac
      361 cggacgcgtc gcggaccacg tcgaggccgg ccgccgcgat ccgcgggccg cgcacggcca
      421 ccccggccat ccacggctcg tagtggtcgg cgctctcgac cacgcgcgcg ggcagtacgc
      481 ccgccgcgac gatgcggcgc tcgccgtaga tgtcggcgag aaacgcgttc agcgcgcgca
      541 cgcgctgcgt cagccccgcg gcgagcccgc tccactcctc ggcggagagc acgcgcggga
      601 ccgggtccac ggcgaacgcg tcgttgtccc cgaagtacgc gccgcgctcg cggaccgcat
      661 ccgcgctggc gctctcaagc acccgcaggt cgcgctgctc gagcgcctcg atcgcggcgc
      721 ggtagtgcgg gtggggctca ccgcgggcgg cgaacgcctc gtcgtagaag cggtcgtcga
      781 cgtcgtagcg ggcgcgctcg gggctcagcg cttccatcgc ttcggcttac ccggcacggc
      841 cggaactcaa agacgcgcgc gagtgcgacc cgcgcctgcg ccgcggcgcg ggcacgcgct
      901 cgcccggcgg gcgcactgcc acggccagct cctcgacggc ctcctcgagc atcgccggca
      961 gccggcccgc gtcgggcagc gcctcgggat cggcgtaggc ggcgaagtgc aggccgccgt
     1021 cgtagctcag cacgccgaac gaaagcgcgt gatcctcggg gatggggatg accgggtaga
     1081 tcgagcgcac ccgcgcgccg gccgcgtaca gcgggatgcg cggtccgggc acgttcgaga
     1141 cggtcaggtt gtagaggcgc gggctggcgg cgagcttcgc ggcgcggcgc ttgagcggct
     1201 ccggtagcga gccgaccgag cgcagcagga tctcggtgcc cgccacgcgg cccgactcct
     1261 tgagctcgcc catctgctcg cgcacgcgtt cgagccgcgc gagtggcgcc ggctcgctca
     1321 ccggcaggtc cacgaacgcg aacgtgatgc ggttgccctc gccgcgctcg tcgtcgggac
     1381 gccggacgct gaccgggacc atcacgcgta ggtcggccgg ctcgtcgccg gtgcgcagcg
     1441 cgagcttcct gagagccccg gcggcgatgg tcagcacgac gtcgttcagg gaggcgccgg
     1501 tctgctgctt gatccggagc gcccggccca tcgagaccct gtgcttgacg agtgtcctgc
     1561 gcggcccgat cggaacgttc agggccgatt tcggcgcggg cttgatcgcg tcctccgcca
     1621 gcgacaccgc cgcgcgcctg agcgtgtccg ccatccggag cccgcttccc ggcgagaggc
     1681 ccagcgcggc gacccggcgc gccgcgcgga actgctcgac ggccgagtcc gcgagcgagt
     1741 cgagcagcac ccggcccggg ccgttgtcgg aagcgggccg ccactcctcg cgcttgaccg
     1801 gcgtcgcctc cggcccagcg tcgaagagca gcagcccgag ttcgaccgcg gcgaggccgt
     1861 cgaccatcgc gtggtggacc ttgcccacga acgcggctcg acggcggtcg accgcgggga
     1921 gcaccaggag gctccagagc ggccggtcgc gcggaagttg ctcggagagg aaggcgtcca
     1981 cggcgcggcg catcgcgggc gcggacagcg gaggcccctc catgaccctc acgtggtcgc
     2041 gcatgtcgaa cgcggggtcg tcgcgccagg ccggctcccc gacccccagc ggcgtctcga
     2101 ccacgcgctg gcgaaagcgc ggcgcaaggt gcaggcgcga ctcgatctgg tcgatcagcg
     2161 ccgccgcgtc gagccgcgcg actccgcgcg ggagctccag cgcggagaac caccccacgt
     2221 gcatgtgggc gctcggcgac tcgacgcgca ggaacgacga gtcgagcgtt gacaaacgcg
     2281 agggcacgct cggatgatcg gcgccggcgc gccgcgcgca catggacccg tgtagaggat
     2341 tccgcggtcg gttttcgacc ggatgtcgaa gtgcttagac gggcgtgcgc tcgccgcgct
     2401 cgaagcgccg ctcgccgcgc ggcgggtcgt cgagccgcgc cgcgggctgt gtcaggagcg
     2461 gccacgtgcg gccgcgctcg tgcatccgga agcgcgcgag caccaccagc gtgcgcagcc
     2521 cgtaggccac gctgtcgcgg aacgagacgc tcgaggcctc gaggaagtag cgcgtcggga
     2581 tcggctgctc gaccacccgc gcgtcgcgcg agacgatctg ggcgaacatc tcctggtcga
     2641 acacgaagcc gtcgctgttg cgcccgaacg ggaccgcgcg caggaagtcc gccgagaagg
     2701 cgcggtagcc ggtgtggtac tcggaatagt cacgacggaa ggcctggttc tcgacccagg
     2761 tgaggaagcg gttgccgacc cacttccagc gcggcatgcc gccggcgatc gtctcgtcct
     2821 cgagcaggcg cgagcccatc accacgtcgg cctcgccgtc ctcgatcggg cggaccatct
     2881 cggcgagcag cgccgggtcg tactggttgt ctgcgtggac catcacgacc acgtcggccc
     2941 cgtcgcggac ggcgcgcgtg taacaggtct tctggttcgc gccgtagccg cggttggccg
     3001 ggtgggcgat cacctcgaag cccgcccgca gcgccgcggt gacggtttcg tcggggctcg
     3061 cgtcgtccac cagcagcgcg cggtcggcgg cccccaccgg catctcctcg gcaacctggg
     3121 cgatcgtcgg ctcggccctg taggcgggca gcaccacgtg cacgtcctgc tcgcgcccgc
     3181 gcggtgcgac cagcggcttc ggcgggcgca gcacctcgac gttgtgcgcg agcacaacgc
     3241 gctcgtcacc cagcgacagc gacaggctgg cgctctcggg cagcgcgcga gcggcggcgc
     3301 gcaccgcggt ccccagggtg ccgccgcgct cggccagccc ggcgagccag tagcgcgcgg
     3361 agaacgtgcg cacgaacgac acgtcgtcgg agatcacgag gccctccgcg gtgagcagct
     3421 cgcgcagcgt gaggcgcggc acgaggcaga cgtgcgccgg cagcagcgcc caccagcgcg
     3481 cgccggccag gcgggcggtg cgcgagcccg ggtcgggggt cacgacgcag agcacgccgc
     3541 cggggcgcag cagctcgcgg cagcggcgga tcgcggccac ggggtcctcc acgtgctcga
     3601 gcacgtcggc gagcacgatc gcggcgaatc cctcgtcggc cggctcgacc tcctcgagcg
     3661 gcgtctccgc cacaggcagg ccgagcacgt cgcgcgcgta gctggcggcg ctgtgggaga
     3721 gctcgacgcc ctcgacctcg tagccgcgac tgcgcgcctc gtcgagcagc agcccgtggc
     3781 cgcagcccac gtccagcagg cgccccgcgg gcacgtgctc accgatcagg tcgagcagcc
     3841 gtcgcgcggt ggcgcggcgc ccgggctcct cggcgaggta ggcctcgtcg ctcatctcgc
     3901 ggtagagccc ggcgagctcg gcggccggcg gcagcgacgg ctgctggacg gtgccgcact
     3961 ccgcgcacgc gtacaggtcg ccgtgggcgc cgggggtgtg gcagctcggg gagaacgact
     4021 cggcgtcggg agccgcggcg ctgccgggat gccggagcac gagtgccccc gcgcagacgc
     4081 ggcacgtgtc aggggcggcc ataacgcgcc gctaggttag ccgcgtgccg gactacggct
     4141 tcccacgcac gagcggcggc ggcaggtccg cgggcggcgt ctgcgagttg agcgccaccg
     4201 cgcgcctgta cgagcggcgg gccgcgtcgt cgtgcccggc cgtcacctgc acgcgcgcca
     4261 gcgtgaccca cacgagctgg ttctccggct cgcgcttgac cacgtcgagc atcagccgct
     4321 cggcctggtc ggcccgcccc gtgcgggcga gcacgatcgc cgtcgagttg tcccgcaggc
     4381 tgtcggggtt cagcttcgtc ctcgccaccg ccagctcggc caggatcgcg ttgtcggccc
     4441 cgccggccac cacggtgcgc accgaggact cgatgcgccc ctcggagcgg tagaccgtgg
     4501 cggacacgag gatccccacc aggcaggcga gcaccagcgc ggcgcgtacg agggacctga
     4561 gcacgctcgc attatgggca ggcgggatct tcgtgatcgc cctgccggtc gtcctggcct
     4621 tcgccaccgg cggctacggg ctgcggcgcg cgtcgttcgc gctcgccgcc gtgctgctgg
     4681 tgctcggcgc cctggccgtg ctcgcgcctt ggccgctcgt gcctcgcggc aagccggcgc
     4741 tggcgctggc cgcgctggcg ggattcgccg cctggaccgc ggtttcgatc gcctgggcgc
     4801 gcgttctggg cgacgccgcc gccgacgcct accgcctcct gctctacgtg gccgcgttca
     4861 tggtcgcgct ctgctgcctg cggctacccg agctgcgccg ccgcgtgccg gacgcgctgc
     4921 tcgccgggat cgtgctggtc gggctctacg ggttggccgg acgactgctc ccagccgtgg
     4981 tcgaagtgcc ggagggcgcg cgccaggacc gcctcaacga gccgttcacc tactggaacg
     5041 cgctcgggat cttcatggtc atggggatcg tcctcgccgt cgccaccgcc agcgacgagg
     5101 agcgcccgcc gtggctgcgc gcgctcgcct gcgcggccgc ggcgccgtgc ggcgtggcgc
     5161 tctacctgac ctactcgcgc gcctcctggc tcgcgctcgc cgccgggctc gtgctcctcg
     5221 tggtcctgcg ccgcacgcgc gcggtgccgc tggcggcggc gctggcgctc gggtcgatcg
     5281 ccgtgctggt ggccgcggtg caggcgttcc cggcggtgct cggcgacgag ggcacgagca
     5341 ccccggcggg cctcgcgttc gcgggcgtga tcggcgtggt gggcgccgcg gcggcactgg
     5401 cgcacctgcg gctctcgcga gcgcgggtcg cgcaagcccc gctgccgatc ggacccgggc
     5461 tcgcgcgtgt tctcgcgata gtgacgatcc cggcggtgat cggagtcggc ctgctcgtgg
     5521 cgaccggcgg cgggaagaac gagaacgtgc gctccggcgg cggccgcgtt gccacgctcg
     5581 acacaaatcg cgacgcgctc tggggcgtcg cgctcgacgc cttcgccgag cacccgctga
     5641 ccggcgtcgg cacgtccagc ttcgcggtcg agtggctgcg cgagcgcgac gaggtggacc
     5701 gcgcgctcga cgctcactcg ctctacgtcg agaccctcgc cgagctgggc atcgtgggcg
     5761 cgctgctg
//
LOCUS       DASVPL010000012         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_157478, whole genome shotgun sequence.
ACCESSION   DASVPL010000012 DASVPL010000000
VERSION     DASVPL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157478"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF517_00560"
     CDS             complement(<1..293)
                     /locus_tag="VF517_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051454.1"
                     /translation="MRIAAADLTRVGPLSPGAKLALALEIVATYARVRRSLRRANVRE
                     TLAELRGGGAGGEAARLAAPAPPGGRGSSPTSPIEGARRLGRVVRRTLALLPG"
     gene            complement(290..1252)
                     /locus_tag="VF517_00565"
     CDS             complement(290..1252)
                     /locus_tag="VF517_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051455.1"
                     /translation="MPAEPARWTARAFGLEIDASFPAPGLPAAGDGEPGRPTRADLVA
                     PEEIDRDWPADGGERVLEEWFDPDTPARTIDTHPEAGYRLYARGFGLARLSPRGDAIQ
                     CSPPDEEPWSWQRFLVGRILPWAAALRGLEVFHASAVAVDGGVLAFVAPTGGGKTSLA
                     IQLVARGGRFFTDDVLALDERDGVLRAHPGAAIAAVRDAEREKIGDDTWRALGSELGH
                     SGKTYMKIPREHAPAPLRAIYYLRPRPEPAIEPIESPGPRLLLSSTFVFGVRTPERLA
                     NQLDVCARIARTVPTFSLGISETVSSAAAAAAVEEHARRAVPAA"
     gene            complement(1242..1547)
                     /locus_tag="VF517_00570"
     CDS             complement(1242..1547)
                     /locus_tag="VF517_00570"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX8051456.1"
                     /translation="MSGAESSGDSMLGQRVRVPEHVVYRDFGDETVILNLQSGTYHGL
                     NETAATMLNALGESDSVAAAIDRLTGEFGQDRAVIERDVLSLCHALSERSLIERDAG"
     gene            complement(1547..2359)
                     /locus_tag="VF517_00575"
     CDS             complement(1547..2359)
                     /locus_tag="VF517_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8051457.1"
                     /translation="MKHAAALITAAGAICKRDFLIFASYRTRFFTTFFTTAVSLTMFF
                     YVSRLVSSPRIGSPDDYYAFVVVGIVIFGVLTSTLSTPVATLRAELQSGTFERMVLSA
                     FGPLRAIVSLLIFPLLLAFATALMSVAYAGIAFGLDIRWSTAAAALPVGLLGAIAFAP
                     FGLLMAAAVVLFKQTNAGATFFITGVTLLAGVYFPVALLPDWIEWASDVQPFTPAVDL
                     LRHVLVGTPLQGSVGVELAKLLGFTAVLMPLSLVTLRAALTRSRRKGTIIEY"
     gene            complement(2356..3015)
                     /locus_tag="VF517_00580"
     CDS             complement(2356..3015)
                     /locus_tag="VF517_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8051458.1"
                     /translation="MASPPLVLKGIVKRWPAVPRVLEGVDLLLEPGSAMAIVGRNGTG
                     KTTLLRIAAGLIAPESGSVSICGLDSERDRTEFQRRTGFVSAGNTGLYARLKVRHHLD
                     MWAKLALLPRAVRTSAIERVAHEFVLEELLGRRVDRMSMGQRQRLRLALAFLHDPMVA
                     LLDEPTTSLDDEGVAILGGAVRALKERGGAAIVCVPGHWEQIPAIDAGFALSDGRLAP
                     A"
     gene            3169..>3571
                     /locus_tag="VF517_00585"
     CDS             3169..>3571
                     /locus_tag="VF517_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051459.1"
                     /translation="MTAGGYPLLGVAGVQDLSFSSPDQPAPGGGGGTGADGATGDPGS
                     PGSPGSPGSPGGPGADGDPGANGDPGANGDPGAAGGDGQDIGGEDISSPGGDGGGSGG
                     GGAGGGSGGGSGGSGGGAAGGKLPFTGHQAGD"
BASE COUNT          505 a         1298 c         1328 g          440 t
ORIGIN      
        1 tcgccgggca gcagcgcgag tgtgcgccgc acgacccggc cgagccgccg cgcaccctcg
       61 atggggctgg tcggcgagga gccgcggccg cccgggggcg ccggcgcggc gagccgcgcg
      121 gcttcgccac ccgcgccgcc cccgcgcagc tccgccagcg tctcgcgcac gttggcccgg
      181 cggaggctgc ggcgcacgcg cgcgtacgtc gcgacgatct cgagcgccag cgccagcttc
      241 gcgcccggcg acagaggccc cacgcgcgtc aggtccgccg cggcgatcct cacgccgccg
      301 gcaccgcccg gcgcgcgtgc tcctcgacgg cggccgcggc cgccgcggag ctcacggtct
      361 ccgagatccc cagcgagaag gtcggcacgg tgcgcgcgat cctcgcgcac acgtcgagct
      421 ggttcgccag ccgttcgggc gtgcgtaccc cgaacacgaa ggtgctcgac aagagcagtc
      481 gcggcccggg gctctcgatc ggctcgatcg ccggctccgg ccgcggtcgc aggtaataga
      541 ttgcccgcag cggggcggga gcgtgctcgc gcgggatctt catgtaggtc ttgccactgt
      601 gaccgagctc ggagccgagc gcgcgccacg tgtcgtcgcc gatcttctcg cgctcggcgt
      661 cgcggaccgc ggcgatcgcc gcgcccgggt gggcgcgcag cacgccgtcg cgctcgtcga
      721 gcgccagcac gtcgtcggtg aagaagcggc cgccgcgcgc gacgagctgg atggcgagcg
      781 acgtcttgcc cccgcccgtc ggggccacga atgcgagcac cccgccgtcg acggcgaccg
      841 cgctcgcgtg gaacacctcg agcccgcgca gggcggcggc ccacggcagg atccgcccga
      901 ccaggaagcg ctgccagctc cacggctcct cgtcgggcgg cgagcactgg atcgcgtcgc
      961 cgcgcggcga cagtcgcgcg agcccgaagc cccgcgcgta gagccggtag ccggcctccg
     1021 ggtgggtgtc gatggtgcgg gcgggcgtgt cggggtcgaa ccactcctcg agcacgcgct
     1081 cgccgccgtc cgcgggccag tcgcggtcga tctcctcggg cgccaccagg tcagcgcgcg
     1141 tcggccgccc cggctcgccg tcgcccgccg cgggcaggcc cggcgcgggg aacgaggcgt
     1201 ctatctcgag gccgaacgcc cgcgccgtcc agcgcgccgg ctcagccggc atcccgctcg
     1261 atcaggctgc gctccgagag ggcgtggcag aggctcagca cgtcgcgctc gatcacggcg
     1321 cggtcctggc cgaactcgcc cgtgagccga tcgatcgccg ccgccaccga gtcgctctca
     1381 ccgagcgcgt tcagcatcgt cgcggcggtc tcgttgaggc cgtggtaggt gccgctctgg
     1441 aggttgagga tcacggtctc gtcgccgaag tcgcggtaca cgacgtgctc gggcacccgc
     1501 acgcgctggc ccagcatcga gtctccgctg ctctccgcgc cgctcatcag tactcgatga
     1561 tggtgccctt gcggcggctg cgggtcagcg cggctcgcag cgtgaccagc gacagcggca
     1621 tcagtaccgc ggtgaatccg agcagcttcg ccagctcgac cccgaccgag ccctggagcg
     1681 gcgtgcccac gagcacgtgg cgcagcaggt ccaccgccgg cgtgaacggc tgcacgtccg
     1741 acgcccactc gatccagtcc ggcagcagcg ccaccgggaa gtacacgccc gcgagcagcg
     1801 tcacgccggt gatgaagaac gtcgcgcccg cgttggtctg cttgaagagc acgacggccg
     1861 cggccatgag cagcccgaac ggcgcgaacg cgatcgcccc cagcaggccg accggcagcg
     1921 ccgccgccgc cgtggaccag cggatgtcga ggccgaaggc gatccccgca tacgccacgg
     1981 acatgagcgc ggtggcgaag gccagcagca gcgggaagat cagcagcgag acgatcgccc
     2041 gcagcggccc gaacgccgag agcaccatcc gctcgaaggt gcccgactgg agctccgcgc
     2101 gcagggtcgc gaccggtgtg gagagggtgg aggtgagcac tccgaagatc acgatcccga
     2161 ccacaacgaa cgcgtagtag tcgtccggcg acccgatccg gggcgacgac acgagccgtg
     2221 atacgtagaa gaacatcgta aggctcaccg ccgtcgtgaa gaaggtggtg aagaagcgcg
     2281 tccggtagct cgcgaagatc aggaagtcgc gcttgcagat cgcgcccgcg gctgtgatga
     2341 gtgctgcggc gtgcttcatg cgggtgccag ccgcccgtcg gagagcgcaa agcccgcgtc
     2401 gatcgccggg atctgctccc agtggcccgg tacgcagacg atcgccgccc cgccgcgctc
     2461 cttgagcgcc cggaccgcgc cgccgagaat cgccacgccc tcgtcgtcga ggctcgtggt
     2521 cggctcgtcc agcagcgcca ccatcgggtc gtggaggaag gccagggcga gtcgcaggcg
     2581 ctggcgctgg cccatcgaca tgcggtcgac gcgccgtccg agcagctcct cgagcacgaa
     2641 ctcgtgcgcg acgcgctcga tcgcggaggt ccgcaccgcg cgcgggagca gcgcgagctt
     2701 ggcccacatg tcgaggtggt ggcggacctt gagccgcgcg tagagcccgg tgttgccggc
     2761 cgagacgaac ccggtgcggc gctggaactc ggtgcggtcg cgctcggagt cgagcccgca
     2821 gatgctgacc gagcccgatt ccggggcgat aagacccgcc gcgatccgca gcagggtggt
     2881 cttcccggtc ccgttgcgcc ccacgattgc catcgccgaa cccggctcga gcagcaggtc
     2941 gacaccttcg aggacgcgcg gcacagccgg ccagcgcttg actatgccct tgagaacgag
     3001 cggggggctg gccatctggg gctattggac cacacgcggc ggggacagcg ccagcacggg
     3061 gtttggcgca tgaaagggcg ccacgggggt accgttcagg gttggatgcg tggatcgcat
     3121 ggaagctatg cgcctccgac ggtctccgac tatggggatc tggcggagat gacggcgggg
     3181 ggttaccccc tgctcggcgt cgcgggcgtg caggatctga gcttcagctc gccggaccag
     3241 cccgctccgg gcggaggcgg cggcacgggt gcggacggcg cgaccggcga tcccggctca
     3301 cccggctctc cgggttcgcc cgggtcgccc ggcggtcccg gggctgacgg cgatcccggc
     3361 gcgaacgggg atcccggcgc caacggcgat cccggcgccg ccggcggcga cggccaggac
     3421 atcggcggcg aggacatctc ctcgccgggc ggcgacggcg gcggctccgg tggcggcggt
     3481 gccggcggtg gctcgggcgg cggctccggt ggctccggcg gcggcgcagc tggcggcaag
     3541 ctgccgttca ccggccacca ggccggtgac g
//
LOCUS       DASVPL010000013        29330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_79584, whole genome shotgun sequence.
ACCESSION   DASVPL010000013 DASVPL010000000
VERSION     DASVPL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 29330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29330
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79584"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..11846
                     /locus_tag="VF517_00590"
     CDS             <1..11846
                     /locus_tag="VF517_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051460.1"
                     /translation="DGASAGLTAPNGPSQERVIRQALASAGLSPADVDAVEGHGTGTT
                     LGDPIEAQALLATYGQERSDGPLRLGSIKSNIGHTSGAAGVAGVIKMVEALRHELLPR
                     TLHAEEPSSHVDWSAGEVRLLTEAEPWERRERPRRAGISSFGVSGTNAHVIVEEAPVE
                     EEAPAERSDPPALPLLLSAHSEVALSAQAERLHGWLTERPELEPLDVAFTLATARARL
                     ERRAAVAVSGREELLDALAALGSGEPARGMTLGEERPGKTAVLFTGQGAQWAGMGAAL
                     YEQFEVFAEALDAVCAELDPRLERPLKELMFAAKGSDEAELLDRTEYTQTALFAIEVA
                     LYRLFESWGLRPDYLIGHSIGELTAAHVAGVLALPDACALVAARGRLMGALPEGGGML
                     AIKAPEDEVAESLAGFEGRLSIAAVNGPRALVVSGEGDALDELEPVWAERGNDVKRLR
                     VSHAFHSRLMEPMLEEFRTVAEGIDYEPPQLEVVSNLTGGLVSDELRDPDYWVRHVRE
                     AVRFADGVGELERLGVTRFVELGPDGVLTAMARMSVGDELGERATFAASMRARRDPLP
                     TLLGCLGALDVAGVELDWHRFFAGSGARLVDLPTYPFQRERYWLEGRGGPGDLVAAGL
                     ENAEHPLLGAAVRLAGEDEGWLFTGRLSTKTQPWLADHAVMDTVLLPGTAMVELALAA
                     AERVGAGGLEELTLVAPLVLDGERALQVAVAEPDDEGRRTIEVYAGPLGSAGDDADDG
                     DWTLHATGLLGADAAADDGALAAFATASWPPEGAEEQDVEFLYDRLAEAGYDYGPTFQ
                     GLRRAYRNGDAWFAEVALDGAADTDGFRLHPALSDAALHTMALGRREEDAPPAVPFTF
                     SGVRLESVGATALRVRIETTGDTDGDATTARVLALDESGAPVFAIEALEARPVDRAAL
                     SARASAGQDGLFALGWVEVEASDDASAPRAALLGEDDGALAGSGIDLEGYPDVVTLEE
                     AIAGGAAAPELVLVRAGAAGGTVHELAERTLELLQAWLGAEALGEARLVVVTDRALTA
                     TAGDSPNLSQAALVGLLRSAESENPGRVALVDLDGSEASAAALPGALASEEPELALRD
                     GVLLAPRLGRAASLTPPPEAGSWRLAIESKGSLDDLALVASEAAETPLAEGEVRVAMR
                     AAGLNFRDVLIALGIYPGEAPLGSEGAGVVVEVGPGVDRLAPGDRVMGFLGEAFASHA
                     VTDQSMLVPIPDGWSYAQAASVPTVFLTAYYALFDLAGVSRGERLLVHGAAGGVGMAA
                     LQLAEHAGAEVFATAHPRKSHTLRELGLSEERIASSRELAFKDEFLDRTGGEGMDAVL
                     DSLSGEFVDASLELLPRGGRFVEMGKADVRDAEEVGREHPGVEYRAFDLMEAGPERIQ
                     EMLAEVVELFERGVLRHLPISTWDIRRGPDAFRHMREARHVGKIVLSVPERPDPDGTI
                     LITGATGGLGALVARHLAAEHGARHLLLTSRRGPEADGAEELVAALAELGCEARVEAC
                     DVADREQVKALLTAIPDEHPLTAVVHAAGVLDDGTIVSLDRERLRAVMTPKVDGAINL
                     HELTRDADLAQFVLFSSVAGTLGSPGQGNYAAANTFLDALAHERSTRGLAATALVWGA
                     WERGMGEGLDDADRARGQRMGMTPLSDEDGLALFDAARAAGDPLLVPVRLDTAALRAG
                     ARDGLLPPILSSIAPAQPRRSRAGGGSLARRLAELPEEGWDEAVLELVRRDVATVLGH
                     GPPDSIDPTRSFKDLGFDSLSAVELRNRLTQTTGLRLSSTLVFDHPTVEAAARFVRSE
                     VAGAERGEPARPSVAARGDEPIAIVGMSCRYPGGVASPEELWELVAEGRDAIGRFPDD
                     RGWDVERLYDPDPDTPGTCYTREGGFLYDAADFDAGFFGVGRSEALAMDPQQRLMLEA
                     AWDALQSAGLDPASIRETETGVFAGAADTNYRRHLGEAYEAFRLTGTTMSVVSGRLAH
                     VFGLQGPAMTVDTACSSSLVALHLACQSLLRGDCSLALAGGVTVMAGPDLLLEFARQR
                     GLAPDGRCKSYAAGADGTGFSDGLGLLVLERLSDAQRLGHRVLALVRGSAVNQDGPSN
                     GLTAPNGPSQERVIRHALASAGLSPADVDAVEGHGTGTTLGDPIEAQALLATYGRERA
                     DGPLWLGSIKSNIGHTSAAAGVAGVIKMVEAMRHGLLPPTLHVDDPSPHVDWSAGEVE
                     LLTEPVDWPAGERPRRAAVSSFGVSGTNAHLILEEAPSTEAPEERSELPALPFPISAH
                     SEHALRAQADRLHGWVAERHELEPVDVAFTLATTRARLERRAAIVGSDREELLEELAK
                     LARGEAGAVEGAKSSGKTAVMFTGQGAQWAGMGAELYERYPRFAEALDAVCAEIDPKL
                     ERPLKELMFAEKGSGEAELLDRTEYTQAALFAIEVALYRLFEAWGLRPDYLIGHSIGE
                     LVAAHVVGMLSLADACALVAARGCLMGTLPEGGGMLAISATEDEVAESLKDYEGRLSL
                     AAVNAPRAVVVSGELEALDQLEPVWKERGANTKRLRVSHAFHSLLMEPMLEDFRQVAE
                     GLDFAEPRIEVVSNLTGGLAPAEMRDPGYWVRHVREPVRFADGVAELERLGVTRFVEL
                     GPDGVLAAMARLSVGEELGDHATFATAMSRKHAQVPTLVGCLAALHGAGAEFDWDAFF
                     EGSGARVVDLPPYAFQRERYWLDRADGAGDLAAAGQLAAEHPLLGATVELAGGEGWVF
                     TGRLSLKTHPWLADHAVMDTVLLPGTAFVELALAAAGRTGAGGVEELTLMAPLVLDGE
                     RAVQVAVAAPDEDGRRTIEIYSRPEAGADDADVEWTLHASGALAADGAAVPGLDDFAA
                     ASWPPEGTEEVDVGGLYDDAAAAGYDYGPAFQGLRRAYRNGDAWFAEVALDGDADGFR
                     LHPGLFDAALHTMLLERGADDGAPPAVPFSFSGVTLARDGAASLRVRIESAGEADDDG
                     ATTIRLLALDDSGAPAFAVEALEARPVDRTALRTQASSLRDALFAVEWTEVESADAAG
                     ASAASNGSGASANGSAARVALLGDGGDGAGLEGEGIELVRHPDLEALEKAIGEGAAAP
                     EVVLVRAPSAAAGAGVHGAAERTLELLQAWLASEALTEARLVLMTDGAVAVAADEAPN
                     LDQAALVGLVRSAHTENPGRFGIVDLDGSEASASRLGETLASEEPELALRRGTLYAPR
                     LARAGAATPPTGASAWRLGIEQKGSLENLALMPSEAAGAALEEGQVRVAMRAAGLNFR
                     DVLIALGIYPGEAPLGSEGAGVVVEVGPGVERLAPGDRVMGFFGEAFGSHAIGEHQML
                     VPIPAGWSYAQAAAVPTVFLTAYYALFDLAEIERGERLLVHGAAGGVGMAALQLAEHA
                     GAEVYATAHPGKWDTLRELGVDDDHIASSRDLDFRERFLSATGGEGMNVVLDSLANEF
                     VDASLELLPRGGRFVEIGKADVREADEVAREHEGVRYRAFDLIEAGPERIQEMLVEVV
                     ELFERGVLRHPPISAWDVRRGAEAFRHMREARHVGKIVLSIPGALEPEGTVLVTGGTG
                     GLGALLARHLAEEHGVRRLVLTSRRGAEAEGAKEVAAALEELGCEAELAACDVADRDQ
                     LEALLARIPDEHPLTAVVHAAGVLDDGLIGSLDAESVRRVMAPKVDGALNLHRLTRDL
                     DLAQFVLFSSIAATVGSPGQANYSAANAFLDALAHHRVTRGLAATAMAWGPWERGMAA
                     GQDEADRARAQRMGMTPLADEEGLALFDASLTAGEAVLVPVRLDTAALRAAGEGLLPP
                     ILSGLIRVRSEGGGDATGSLQRRLSGVPESKWSAIVLDMVRDQVASVRGLESGDAVKP
                     DLAFKEMGFDSLAAVELRNGLTRSTGIRLPATLVFDHPTPSAVAEHVLGRLPRPDAGP
                     SIEDEFDRIERLLHDASMDERTRSRMEARVRTFNSRVQSLLMGSSDGDDGDRDEDLGS
                     VSNEEVFALIDKEIGAS"
     gene            11843..19156
                     /locus_tag="VF517_00595"
     CDS             11843..19156
                     /locus_tag="VF517_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051461.1"
                     /translation="MSDEQRLNEYLRKATGELRAARRRVHELEQRDHEPIAIVGMSCR
                     YPRTVRSPKELWELVATGAEAVGPYPDDRGWDIDHLYDPDPDHIGTVFAREGAFLEGI
                     AEFDAEFFGIGPREAVAMNPQQRIMLETAWEAFEDAGIDPASLRGSRTGVFAGVFHDD
                     YVSGPARHPSSADAERFASTGEPTSLVSGRVAYAFGLEGPTMSVDTACSSSLVAMHLA
                     CHALRDRECSLALAGGVTTMSTTMMLRAFSRQRALSPDGRCKAFGADADGTGFSEGGG
                     LLVLERLSDARRRGHRVLAVMRGSAVNQDGASNGLTAPNGPSQERLIRDALADAGLSP
                     ADVDVVEGHGTGTTLGDPIEAQALLATYGQERSNGPLYLGSIKSNIGHTSAGAGVAGV
                     IKMVEAMRHDLLPRSLYCEEPSTHVDWSAGAVELLREPVEWPAGERPRRAGVSSFGVS
                     GTNAHVIIEEAPVEEEAPAERSELPALPFLVSGRSQAALRAQAERLRDWLTERPELEP
                     LDVAFTLATGRAKLQHRAALIASDRDDLVAGLEALAGGGSRPGMAVGEERPGKTAFQF
                     TGQGAQWPGMGAEFCERFPAFAEALDAVCGELDPHLERPLKELIFAAEDSPEAELLGS
                     TEFTQAALFAIEVALYRLFESWGVRPDYLIGHSIGELVAAHVAGVLSLSDACTLVAAR
                     GRLMGALPEDGAMLAIAASEHEVAGTLAELGGAVSLAAINSPLSVVVSGEREAVESCE
                     ATWSELDRKVSRLRVSHAFHSHLMEPMLDELRKVAEGLTFEAPQVEVISNLTGGLVSD
                     ELRDPDYWVRHVRETVRFADGVATLTRLGVTRFVELGPDGVLSAMARQSVSAELGKRA
                     VFAPAMSSRREPLATVLGCLGALHAAGGEVDWPAFFEGSGGRLVDLPTYAFQRERYWL
                     EAAGEVGEASAGDLTAAGQLAVEHPLLGAAVRLAAGQGGWLFTGRLSSQTHPWLFDHT
                     VTGVSVMPGTGFVELALAAAQRVGAEGVEELTLVAPLAFDEEGAVRLQVAVSEPDDDG
                     RRAIGVYSSPTADASDENGSTDWVLHATGRLGAPGATAPESAEAFRAEWPPADAQEVD
                     VESFYDRAAEAGYDYGPAFQTLRRAYRNGEASFAEVGLGETVQSDAGRFRVHPALFDG
                     ALHAMLLEALEGAGTDGAPRVPFAFSRVSLHREGAAALRVRIETTTDDASDATTLRLL
                     AVDESGAPVLVVDAIDVRPFDQATLAEAARKARPQAGEGSLLTVEWTGVEAAGSDDPW
                     LEAALLGDDDGVLEGSGLSLDPYADFEALEQALAGGTTAPEMVLMRPGAADGPLAGSV
                     AEFTQRALELLQAWLDSEALADTRLVFVTDRALAVAPGESPNLSQAALVGLLRSARSE
                     HPGRFALVDLDGSDESVVALFGALTSDEPELAVRNGVLYAPRLARAAGLTMPAGEGAW
                     RVASERKGSLEDLAIVTSNAGDDALAEGEVRVAMRAAGLNFRDVLIALGMYPYEAPLG
                     SEGAGIVVEVGPGVERLSPGDRVMGFVGEAFGSHAVTDARFLVPLPDGWSFVEAASVP
                     TVFLTAYYALFDLAELQSGERLLVHGAAGGVGMAALQLAEHIGAEVFATAHKRKWGTL
                     RDLGVERSHIGSSRELTFRERFLKATDGEGVDVVLDSLANEFVDASLELLPRGGRFIE
                     IGKADIREPDTVAAEHQGVRYRAFDLSDVEPERVQELLLEVVRLFEAGALRHLPIQSW
                     DVRSAPEAFRYMREARHVGKIVLTVPQQPDPEGTILITGATGGLGELVARHLADHHGA
                     RHLLLASRRGEEAGGARELVDALAEHGCEAKLAACDVADRGQLEKLLAEIPADHPLTA
                     VIHAAGTLDDGVIGSLDAERLRGVMAPKVDGAINLHELTADGELAQFVLFSSIAGTLG
                     TPGQANYAAANAFLDALAHDRSAQGLAAKSLAWGAWERGMAAGLSDTDRQRGERIGIG
                     ALSDEDGLALLDAANATAEPQLAPLAIDTKALSAHAGSGLLPPMLQGLVRESAREDRG
                     DAQNSLGQRLAAAPKSEWESIVVDLVRSEVASVLGHSSSDAVDPEREFKELGFDSLAA
                     VELGNRLTDATGLAIQPSVTFDLQTPHALAAYLAERLRDEAPDVPEGAPAAQESPGTP
                     VTQGAPADGAPVKRPSTAGTLTALLRGAHEQGTLGDFVPAAMATSKFARAFRSPAELE
                     HLPSLVPLSRGAGTRLICVPSFLAGSGPHQFSRLASHLDGVRTMSALSLPGFRTGELV
                     PGTWDALIEALAASVREAATDDPFVLVSYSHGGGPAHSLAGKLEEQGLAPAGLVMIDT
                     YAPESEEEMRQLFRKVVETIFENDHPLIEGSVDDGNLLAMGAYFRLAEEWEATPIETP
                     HLLIRATEPLGDAYEGGRLPWWQLPSEVTEVTGNHFGLIDQSAGETAQVIDAWVREKT
                     GEPAPTRDAEVARAG"
     gene            19251..19952
                     /locus_tag="VF517_00600"
     CDS             19251..19952
                     /locus_tag="VF517_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8051462.1"
                     /translation="MAATPKSAPPPRNGAGVTDPIADAVNRGDYDLFAQALAEDVVFR
                     SPVSRFRFRGPEITSALFERLVKQSDPDRWGVQDSWSLAPDAHAMALTTNVRGHQLDL
                     LIVTRLNERHQISQVTAYARPMASIAIFPAFVYPHLVERFRGPGRAAVVRTVFRPLPR
                     LLKVLVSAGLGFGRPPQAEFEEAELPTREEPPAAPSAGPSSASDGGQSSGGPRPSSDG
                     AQSPPAGPRSPFRRL"
     gene            20026..21786
                     /locus_tag="VF517_00605"
     CDS             20026..21786
                     /locus_tag="VF517_00605"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEX8051463.1"
                     /translation="MENRVHDVVVMGGGQAGLSLTLQLKQRDPDIDILVVERSEHPPP
                     SAAHKVGESTVEGGAYYFSKILGLKPYLDKEQLRKSGLRYYFSDGRNTDLARRVELGN
                     SRQLPFATYQLDRGTFEAKLGSLVSEAGAEFLDKAVVRDVTLGEGDDPHRIQIKRDGV
                     ESEVQARWVVDATGRFQFLKRKLDLAKQVDHKMNASWFRLPAKVVVTDWSDDPDWQNR
                     VGKFVLSDGTPAQLRSLSTVHLVGHGWWLWLIPLAGDVTSVGIVVDDRVFPISEINSL
                     EKSMAWIEEHEPQAYEAIAPHIDDVLDFKFLRHYSHSCERILSPERWCITGVAGAFHD
                     PLFSVGADIICWNNTIITDVIARDHAGEDIGSRVQLYNSIFLDQYVGPMYALFEDTYL
                     YMGNPQVFSVWVHWSTCWYWSILASIITHDKITDLETLSTLHDEVKRSIDLWHVMQKF
                     FTEWWEAAGDDYSHTDHFIPLFNQPWINRLQADLLADWDSEEFTQKLRENLENLEAAV
                     AYVFWMAVEGLPDPPERRPIDPYKITMDRDRWEADGLFDTDGVTPSNPVDLDAEFGGY
                     GWFSRDRAGAAEEAALSQSS"
     gene            complement(21800..23098)
                     /locus_tag="VF517_00610"
     CDS             complement(21800..23098)
                     /locus_tag="VF517_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8051464.1"
                     /translation="MNFALSDEALEFRDLCRRFAREVIRPAAAKHDAEESTPWEVIRA
                     SHEWGLNGLERMGQMGADPTGQCLVILPEEFHWGCAGIALAITGSVLATSGIVAAGAP
                     EQIAQWMPECLGTAEEPKLGAYGVTEPEAGSDLKSLKTRAELDGDEWILNGTKVFITN
                     GGIADVHVIVASVDPELGQEGQASFVIPKGTPGLRQGKKESKLGIRASHTAEVVLEDC
                     RVPVENLLGGMDRLNERLERARSGASRGQSSNAMATFEVTRPMVGAAAVGIAQAAYDW
                     TLDFLAEGGELNPIRTALDESSTAGEPPLRRQEVQQIIADVATEIDAARLLVQRAAWM
                     GRNGIPMTGGQGSMSKLKGGNVAVWATTTLMDLVGPYAQTADCPLEKFFRDAKVYQLF
                     EGTAEIQRLVVARMQVARREREGAVRAPAAAEPALDPAAA"
     gene            23218..23895
                     /locus_tag="VF517_00615"
     CDS             23218..23895
                     /locus_tag="VF517_00615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8051465.1"
                     /translation="MRAIDITAPPLEPEELTDRLRRAPWRRYVTLGDSLVEGIGDPVE
                     GYPEMGWAEATGRTFKELHADFEFLNLGKRFLTADRVRMTQLQPALDFEPDLAAVLAG
                     GNDLGDPFDPGGIERELDRMVAALSATGATVMVWSVLNMMSCGRYPEPMVDLLAPRLA
                     AHRDISLRVAERYDTVFFDCFNHPMCADPSILSEDLLHINMRGQALLTEMTLQGLASL
                     VPARTAA"
     gene            complement(23908..24081)
                     /locus_tag="VF517_00620"
     CDS             complement(23908..24081)
                     /locus_tag="VF517_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX8051466.1"
                     /translation="MTTQTQQLWICQECGFIYDPAEGDPDGGIPPGTPFEDIPMDWVC
                     PACGVHKDDFVPY"
     gene            complement(24126..25004)
                     /locus_tag="VF517_00625"
     CDS             complement(24126..25004)
                     /locus_tag="VF517_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8051467.1"
                     /translation="MTADDGTRIYYELFSPEPDLAREGAAPVLLLMGLGGNGRLWGPA
                     VRRLLGDGHDVIAVDNRGCGRSGTPWLPFTTSTMAADAVAVLDQLGIERAHIGGASFG
                     GMIAQEIALEFPERVGALVLLCTTGGLPRMDLSLLPRLDLVPRRGILPIFGTVLRSFR
                     PASDPEQRVRDFLSMAASQDYVANCRPEDEAWAAVAASLEVPSSQRGLVMQVLAAAGH
                     SSWSRLSRLRMPVLVHHGTEDRIVPIAAGEELARRIPTARFEAYEGAGHGIFERLDES
                     SGSILAFLGECDPLVA"
     gene            complement(25073..25783)
                     /locus_tag="VF517_00630"
     CDS             complement(25073..25783)
                     /locus_tag="VF517_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX8051468.1"
                     /translation="MPEHRYQQYCGLARALDVAGDRWTLLIVRELVPGPRRFTDLIDG
                     LPGISRNLLTERLRALERDGVIAREDLPPPAARQVYGLTADGRDLAEAMTGLIGWGAR
                     RLGERDPTDSFRPRWAAMAMANFADREAARGVSETYQYLVGRLAFYFTIDDGAIRLRD
                     GFAHDPAVTLTTDEETWEAVTSGKVTAASAAATGALTVAGDPRAAKRLGNIFSRHHLL
                     ALIGATDHRNNPELEHST"
     gene            complement(25793..27973)
                     /locus_tag="VF517_00635"
     CDS             complement(25793..27973)
                     /locus_tag="VF517_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8051469.1"
                     /translation="MERLAAGVRRWRWAVLAAWLVLVVVAAPLAARQSEDTVAGGNRV
                     PGSESEQVREALARDFDERAQLAVVLAPKPGSKAADRVQAVDALLRSIQDVPEATLPG
                     STRGRARAAAERGRTAILPVGVLASQDRESEVAKDIHDRLRGVDGAGRSVSSHVIGRG
                     ALDVAIQEEATTQLERAETIGLPIVLLILLTIFGSLAAAALPVVLGIAAVLITGAAIY
                     LASQEFLVSVFATNSASLFGIALAVDYSMFILVRFREEIRAGRDRDAAISRALSTSGV
                     AIVFSGLTVVVAIGAIAIVDNAGMRSMVGAILFTVITAVLLSAVLLPVLMRLLGNRLV
                     QQRRLPLLSRRSAKPATGSRPSFWERWTRVVLGRPILFATAATLTLLVLALPMLSLDP
                     GEHLVGQLPRDNDTRVGYDRAAAEQGPGATSPVQVVLQSERPQPVEREAVASARATIA
                     RDPEVAGVAPPKAAEGGRSVLLTATPRSDGSSPSTEELVRRLRSEFDEPVSPGVTAQV
                     GGVTAYSIDFQDQIFGNLWKVAVLGLAFSFVLLTVLLRSLVLPLKAVILNLLTVGAAY
                     GAVVAVFQWGWLDGFLGFKSEGSVNTFGLLPIMTGVFALSMDYEIFLMSRIREQYERT
                     GDPSSAIAQGLAMSARPITGAAVIMVALFCVFASAGLTSVREFGLGLAVGIALDATVV
                     RLVLVPSTMQLLGRRNWWLPRPLARVLDNRVFDEAKATTPLPDA"
     gene            28160..28669
                     /locus_tag="VF517_00640"
     CDS             28160..28669
                     /locus_tag="VF517_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8051470.1"
                     /translation="MAETTTAAGALTNEWVEEFGERYFAAWDSHDPDRLLELMTEDVV
                     YEDSAWPMPMNGHAEVREFLEFVYRAFPDIRFEHEVPLIAADEPRAAFPCVGWATNSG
                     PIEPPGIPATGEKLDWKGIDIIDFRDGMVSRLRILYNPAPGWLQLGLLQGVELDGGET
                     ARRTLGETE"
     gene            complement(28715..29191)
                     /locus_tag="VF517_00645"
     CDS             complement(28715..29191)
                     /locus_tag="VF517_00645"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8051471.1"
                     /translation="MNQTPDDRHEQWSTLAVGNPARERLLDTLAADAFDAHLGTEWLD
                     LGPDEARSRIPVENHHKGPVGFVHGGVFATMAEGICSAATYAAVINDGMTTWGQSNDT
                     TFLRPISEGHLNAVARPRHRGRTTWVWDVEISDDDERLCALVRMTIAVRPAPRAAG"
BASE COUNT         4092 a        10374 c        10684 g         4180 t
ORIGIN      
        1 aggacggcgc atcggccggg ctgaccgcgc cgaacggccc ctcgcaggag cgggtcattc
       61 gccaggcgct ggcgagcgcc gggctctcgc ccgccgacgt cgacgccgtg gagggccacg
      121 ggacgggcac cacgctcggc gacccgatcg aggcccaggc gctgctcgcc acctatggcc
      181 aggagcgctc cgacgggccg ctgcggctcg gctcgatcaa gtcgaacatc ggccacacct
      241 cgggggcggc gggcgtcgcc ggggtgatca agatggtcga ggcgctccgc cacgagctcc
      301 tgccgcggac gctccatgcc gaggagccct cgtcgcacgt cgactggtcg gcgggcgagg
      361 tgcgcttgct gacggaggcc gagccgtggg agcggcgcga gcgaccgcgc cgggcgggga
      421 tctcgtcgtt cggggtctcg ggcacgaacg cccacgtgat cgtggaggag gcgccggtcg
      481 aggaggaggc gccggccgag cgctccgacc ctcccgccct gccgctgctc ctctcggctc
      541 acagcgaggt ggccctgagc gcccaggccg agcgcctgca cggctggctc accgagcggc
      601 cggagctcga accgctcgac gtcgccttca cccttgccac ggcccgggcc aggctcgagc
      661 gccgggcggc ggtggcggtc tcgggtcgcg aggagctgct ggacgcactc gcggcgctgg
      721 gcagcggcga gcccgcgcgc gggatgacgc tcggcgagga gcggcccggc aagaccgcag
      781 tcctcttcac cggccagggc gcccagtggg cggggatggg cgcggcgctg tacgagcagt
      841 tcgaggtctt cgccgaggcg ctcgacgcgg tgtgcgcgga gctcgacccg cggctggagc
      901 ggcccctgaa ggagctgatg ttcgccgcca agggctccga cgaggccgag ctgctcgacc
      961 gcacggagta cacgcagacc gcgctgttcg cgatcgaggt cgccctctac cggctgttcg
     1021 agtcgtgggg cctgcggccc gactacctga tcggccactc gatcggcgag ctcacggcgg
     1081 cccacgtcgc cggcgtcctc gcactcccag atgcctgtgc tctggtcgcc gctcgcgggc
     1141 gcctgatggg tgcgcttccg gagggcggcg ggatgctcgc gatcaaggca cccgaggacg
     1201 aggtcgcgga gtcgctcgcc ggcttcgagg gccggctgtc gatcgcggcg gtcaacgggc
     1261 cgcgtgcgct cgtggtgtcg ggcgaggggg acgcgctcga cgagctcgag ccggtgtggg
     1321 ccgagcgcgg gaacgacgtc aagcgcctgc gcgtgagcca cgccttccac tctcggctga
     1381 tggaacccat gctcgaggag ttccgtacgg tcgccgaggg catcgactac gagccgccgc
     1441 agctcgaggt ggtctcgaac ctgaccggcg ggctggtctc cgacgagctc cgcgatccgg
     1501 actactgggt ccgccacgtg cgcgaggcgg tccgcttcgc cgacggcgtg ggcgagctcg
     1561 agcggctcgg cgtgacgcgc ttcgtcgagc tcggcccgga cggggtgctg acggcgatgg
     1621 cgcggatgtc ggtcggcgac gagctcggcg agcgggcgac gttcgcggcg tcgatgcgtg
     1681 cccgccgcga cccgctgccg acactgctcg gctgcctcgg cgcgctcgac gtggccggcg
     1741 tcgagctcga ctggcaccgc ttcttcgcgg gcagcggcgc gcgcctcgtc gacctgccca
     1801 cctacccctt ccagcgcgag cgctactggc tcgagggccg gggtggcccg ggcgacctcg
     1861 tggcggcggg gctggagaac gccgagcacc ccctcctcgg ggcggcggtc cgcctcgccg
     1921 gcgaggacga gggctggttg ttcaccgggc gcctttcgac caagacccag ccctggctcg
     1981 ccgaccatgc cgtgatggac acggtcctcc tgccggggac cgccatggtc gagctggcgc
     2041 tcgccgccgc ggagcgcgtc ggcgcgggcg ggctcgagga gctgacgctc gtcgcgccgc
     2101 tggtcctcga cggcgagcgg gcgctccagg tggccgtggc cgagcccgac gacgaggggc
     2161 gccggacgat cgaggtctac gcaggcccgc tgggcagcgc cggcgacgac gcggacgacg
     2221 gggactggac gctgcacgcg accggcctgc tcggcgccga tgcggccgct gacgacgggg
     2281 cgctcgcggc cttcgccacg gcgagctggc cgcccgaggg cgccgaggag caggacgtcg
     2341 agttcctcta cgaccgcctg gccgaggccg gctacgacta cgggccgacc ttccagggcc
     2401 tgcgccgcgc ctaccgcaac ggcgacgcct ggttcgccga ggtggccctc gacggcgccg
     2461 ccgacacgga tggcttccgc ctccacccgg cgctgtccga cgcggcgctg cacaccatgg
     2521 ccctcggccg ccgcgaggag gacgcaccgc ccgcggtccc cttcaccttc tccggggtgc
     2581 gtctcgagag cgtgggcgcc accgcgcttc gggtgcggat cgagacgacc ggcgacaccg
     2641 atggcgacgc caccaccgcg agggtgctgg cgctcgacga gtccggcgcg cccgtcttcg
     2701 cgatcgaggc gctcgaggca cgccccgtcg accgggcggc gctcagtgcc cgggcgagcg
     2761 cgggccagga cgggctgttc gcgctcgggt gggtggaggt cgaggcatcg gatgacgcct
     2821 cggcgccgcg cgccgccctc ctcggcgagg acgacggcgc cctggccggc tccggcatcg
     2881 acctcgaggg ttatcccgac gtcgtgacgc tcgaggaggc gatcgccggc ggggcggccg
     2941 cccccgagct ggtgctggta cgtgccggcg ccgccggcgg gaccgtccac gagctggccg
     3001 agcggaccct cgagctcctg caggcgtggc tcggggcgga ggcgctgggc gaggcgcggc
     3061 tcgtcgtcgt caccgaccgc gcgctgacgg ccacggcggg cgactcgccg aacctctccc
     3121 aggccgcgct ggtcggcctc ctgcgcagcg ccgagtcgga gaacccgggc cgcgtcgccc
     3181 tcgtcgacct cgacggaagc gaggcgtcgg cggccgctct ccccggcgcg ctcgccagcg
     3241 aggagcccga gctggccctc cgggatggcg tcctcctcgc gccgcggctg ggccgcgccg
     3301 ccagcctcac cccgcccccc gaggcgggtt cgtggcgcct cgccatcgag agcaagggct
     3361 cgctcgacga cctggcgctc gtcgcatccg aggccgccga gaccccgctc gcggaggggg
     3421 aggtccgcgt ggccatgcgc gccgccgggc tcaacttccg cgacgtcctg atcgccctcg
     3481 gcatctatcc cggcgaggcc ccgctcggca gcgagggggc cggcgtcgtg gtcgaggtcg
     3541 ggcccggcgt cgatcgcctc gcgcccggag atcgtgtgat gggcttcctc ggcgaggcct
     3601 tcgcctcgca cgcggtcacc gaccagtcga tgctcgtgcc catccccgac ggctggtcct
     3661 acgcccaggc ggcctcggtt ccgacggtct tcctgaccgc ctactacgcg ctgttcgacc
     3721 tggctggggt ctcccgcggc gagcggctgc tcgtccacgg cgccgccggt ggggtcggca
     3781 tggccgcgct gcagctcgcc gagcacgccg gggccgaggt cttcgccacc gcgcacccgc
     3841 gcaagtcgca cacgctgcgc gagctcggcc tgagcgaaga gcggatcgcc tcctcgcgcg
     3901 agctggcgtt caaggacgag ttcctcgacc gcaccggcgg cgagggtatg gacgcggtgc
     3961 tcgactcgct gagcggcgag ttcgtggacg cctcgctcga gctcctcccg cgcggagggc
     4021 gcttcgtcga gatgggcaag gccgacgtgc gcgacgccga ggaggtcgga cgcgagcacc
     4081 cgggtgtcga gtaccgcgcc ttcgacctga tggaggctgg tcccgagcgc atccaggaga
     4141 tgctcgccga ggtggtcgag ctcttcgagc gcggcgtcct ccgccaccta ccgatctcga
     4201 cctgggacat ccgccgcggc cccgatgcct tccgccacat gcgcgaggcg cgccacgtcg
     4261 gcaagatcgt cctgagcgtc cccgagcggc cggaccccga cggcaccatc ctcatcaccg
     4321 gcgcgacggg cggccttggc gcgctcgtgg ctcgccacct ggcggccgag cacggcgcgc
     4381 gccacctgct cctgacgagc cgccgcgggc ccgaggccga cggtgcggag gagctcgtcg
     4441 cggcactcgc cgagctcggc tgcgaggccc gggtcgaggc ctgcgacgtc gccgaccgag
     4501 agcaggtcaa ggcccttctc accgcgatcc ctgacgagca cccgctgacc gcggtcgtcc
     4561 acgcggccgg ggtgctcgac gacggaacga tcgtctcgct cgaccgcgag cggctcagag
     4621 cggtcatgac cccgaaggtc gacggcgcga tcaacctgca cgagctgacc cgcgacgccg
     4681 acctcgccca attcgtcctg ttctcctcgg tagccgggac gctcggctcg ccggggcagg
     4741 ggaactacgc ggccgccaac accttcctcg acgcgctggc acacgagcgc agcacacgcg
     4801 ggcttgcggc caccgcgctc gtgtgggggg cgtgggagcg cgggatgggc gagggcctgg
     4861 acgacgccga ccgggcacgg gggcagcgca tgggcatgac gccgctctcg gacgaggacg
     4921 ggctcgcgct gttcgacgcc gcccgcgccg ccggcgaccc gctgctcgta ccggtgcgcc
     4981 tcgacaccgc ggccctgcgc gcgggggcgc gcgacgggct gctgccgccg atcctgagct
     5041 cgatcgcccc ggcccagcca cggcgctcgc gcgcgggcgg tggatcgctc gcgcggcggc
     5101 tcgccgagct gcccgaggag gggtgggacg aggcggtgct cgagctcgtt cgcagggacg
     5161 tggccacggt gctcggacac ggcccgcccg attcgatcga tcccacgcgc tcgttcaagg
     5221 acctcggctt cgactcgctc tccgcggtcg agctgcgcaa ccggctgacg cagaccaccg
     5281 ggctgcgcct gtcgtcgacg ctggtgttcg accacccgac ggtcgaggcc gcggcacggt
     5341 tcgtgcgctc ggaggtcgcc ggcgccgagc gtggtgagcc cgcgaggccg agcgtggccg
     5401 cgcgcggcga cgagccgatc gccatcgtcg ggatgagctg ccgctatccg ggcggggtcg
     5461 cctcgcctga ggagctgtgg gagctcgtgg ccgagggccg cgacgcgatc ggccggttcc
     5521 ccgacgaccg tggctgggac gtggagcggt tgtacgaccc cgacccggac accccgggca
     5581 cctgctacac ccgcgagggc gggttcctct acgacgcggc ggacttcgac gcgggcttct
     5641 tcggggtcgg gcggagcgag gccctggcga tggatcccca gcagcggctg atgctggagg
     5701 cggcgtggga cgcgctccag agcgcgggcc tcgatccggc ctcgatacgc gagaccgaga
     5761 ccggcgtgtt cgccggtgcg gcggacacga actaccggcg gcacctgggc gaggcgtacg
     5821 aggcattccg gctgaccggc accacgatga gcgtggtctc gggccggctc gcccacgtgt
     5881 tcggcctcca gggcccggcg atgaccgtcg acacggcgtg ctcgtcgtcg ctcgtggcac
     5941 tgcacctcgc gtgccagtcg ctgctccgcg gagattgctc gctggcactg gccggcggcg
     6001 tgaccgtgat ggccggaccc gacctgttgc tcgagttcgc gcggcagcgc gggctggctc
     6061 cggacggccg gtgcaagtcc tacgcggccg gggcggacgg cacggggttc tccgacggcc
     6121 tcggcctcct cgtgctcgag cggctatcgg acgcgcagcg gctcggtcac cgcgtcctgg
     6181 cgctcgtgcg cggcagtgcg gtcaaccagg acgggccgtc gaacggcctg acggcgccga
     6241 acgggccgtc gcaggagcgt gtcatccgcc atgcgctggc gagcgcgggc ctctcccccg
     6301 ccgacgtcga cgcggtcgag gggcacggca ccggcacgac gctgggcgat ccgatcgagg
     6361 cgcaggcgct gctcgcgacc tacggccggg agcgcgcgga cgggccgctg tggctggggt
     6421 cgatcaagtc gaacatcggg cacacctcgg cggctgccgg cgttgcgggc gtcatcaaga
     6481 tggtcgaggc gatgcggcat ggcctgctgc cgccgacgct gcacgtggac gacccgtcgc
     6541 cgcacgtcga ctggtcggcc ggcgaggtcg agctgctcac cgagccggtc gactggccgg
     6601 cgggcgagcg cccgcgccgg gccgccgtgt cgtcgttcgg ggtctccggc acgaacgcgc
     6661 acctgatcct cgaggaggcg ccctcaaccg aggcgccgga ggagcgctcc gagcttccgg
     6721 cgctcccgtt cccgatctcc gcccacagcg agcacgcgct gcgagcccag gccgatcggc
     6781 tgcacggctg ggtcgcggag cggcacgagc tcgagccggt cgacgtggcg ttcacgctgg
     6841 cgacgacgcg cgcccggctc gagcgccggg cggcgatcgt cgggtcggac cgcgaggagc
     6901 tcctcgagga gctcgcaaag ctcgcgcgcg gcgaggcggg cgccgtcgag ggcgcgaagt
     6961 cctccggcaa gaccgccgtg atgttcaccg gccagggcgc gcagtgggcg gggatgggcg
     7021 ctgagctcta cgagcgctac ccgcggttcg ccgaggcgct cgacgcggtg tgcgccgaga
     7081 tcgacccgaa gctcgagcgg ccgctcaagg agctgatgtt cgccgagaag ggctccggcg
     7141 aggccgagct gctcgaccgc accgagtaca cgcaggccgc gctgttcgcg atcgaggtcg
     7201 ccctctatcg cctgttcgag gcgtgggggc tgcgacccga ctacctgatc gggcactcga
     7261 tcggcgagct cgtcgcggct cacgtcgtcg ggatgctctc gctcgccgac gcctgcgcgc
     7321 tcgtggcggc gcgcggatgt ctgatgggca cactgcccga gggcggcggg atgctcgcga
     7381 tctcggcgac cgaggacgag gtcgccgaga gcctgaagga ctacgagggt cggctgtcgc
     7441 tcgccgccgt caacgcgccg cgcgcggtgg tcgtctccgg cgagctcgag gcgctcgacc
     7501 agctcgagcc ggtctggaag gagcgcggcg ccaacacgaa gcgcctgcgc gtgagccatg
     7561 ccttccactc gctgctgatg gagccgatgc tggaggattt ccgccaggtg gcggagggcc
     7621 tcgatttcgc ggagccgcgg atcgaggtcg tctcgaacct gacgggcggc ctcgccccgg
     7681 ccgagatgcg tgaccccggc tactgggtcc ggcacgtacg cgagccggtg cgcttcgccg
     7741 acggggtcgc cgagctcgag cggctcggcg tcacccgctt cgtggagctg ggcccggacg
     7801 gcgtgctcgc cgcgatggcg cgcctgtcgg tcggcgagga gctcggcgac cacgccacct
     7861 tcgcgaccgc gatgagccgc aagcacgcgc aggtcccgac gctcgtcggc tgcctcgccg
     7921 cgctccacgg cgccggcgcc gagttcgact gggacgcgtt cttcgaaggc agcggcgcgc
     7981 gcgtcgtcga cctgccgccc tacgccttcc agcgcgagcg ctactggctc gaccgcgccg
     8041 acggggcggg cgacctcgcg gccgccggcc agctcgcggc cgagcatccg ctgctcggcg
     8101 cgacggtcga gctcgcgggc ggcgagggct gggtgttcac cgggcgccta tcgctcaaga
     8161 cccacccgtg gctcgcggac cacgcggtga tggacacggt gctgctgccc ggcaccgcgt
     8221 tcgtcgagct ggcgcttgcc gccgccggac gcaccggtgc cggcggggtc gaggagctga
     8281 cgctgatggc gccgctcgtg ctcgacggcg agcgggcggt gcaggtggcg gtggccgcgc
     8341 ccgacgagga cggccgccgg acgatcgaga tctactcgcg cccggaggcc ggggccgacg
     8401 acgccgatgt ggagtggacg ctgcacgcct cgggcgccct cgccgccgac ggcgctgccg
     8461 tccccggcct cgacgacttc gccgccgcga gctggccgcc ggagggcacg gaggaggtcg
     8521 acgtcggcgg cctctacgac gatgcggccg cggccggcta cgactacggc ccggcgttcc
     8581 aggggctgcg ccgggcatac cggaacggcg acgcctggtt cgcggaggtg gccctcgacg
     8641 gcgacgccga cgggttccgc ctccacccgg ggctcttcga cgcggcgctg cacaccatgc
     8701 tgctcgagcg cggtgcggac gacggcgcgc cgcctgccgt gccgttctcc ttctccggcg
     8761 tgacgctcgc gcgcgacggg gccgcctcgc tgcgcgtgcg gatcgagtcg gctggcgaag
     8821 cggacgacga cggcgccacc acgatccggc tgctggcgct cgacgactcc ggcgcccccg
     8881 cgttcgccgt cgaggcgctc gaggcgcgtc cggtcgaccg gacggcgctg aggacccagg
     8941 ccagctccct ccgggacgcg ctgttcgcgg tcgagtggac ggaggtcgag agcgcggacg
     9001 ccgccggcgc gagcgcggcc tcgaacggct ccggcgcttc cgcgaacggg tcggcggcgc
     9061 gcgtcgcgct gctcggcgac ggaggcgacg gcgccggtct cgaaggggaa gggatcgaac
     9121 tcgtgcgcca cccggatctc gaggctctcg agaaggcgat cggcgagggg gccgcggcgc
     9181 ccgaggtcgt gctcgtccgc gcgcccagcg cagccgccgg ggccggcgtg cacggcgccg
     9241 ccgagcggac cctcgaactg ctgcaggcat ggctcgcgag cgaggccctg accgaggcgc
     9301 gactcgtgct gatgaccgac ggcgcggtcg ccgtcgcggc cgacgaggca ccgaacctcg
     9361 accaggccgc gctggtcggc ctggtgcgga gcgcccacac cgagaacccc ggccgcttcg
     9421 gcatcgtgga cctcgacgga agcgaggcct cggcgagccg cctcggcgag accctcgcga
     9481 gcgaggagcc ggagctggcc ctgaggcgcg gcacgctgta cgcgccgcgg ctcgcgcggg
     9541 cgggcgccgc gaccccgccc accggcgcgt ccgcgtggcg gctcgggatc gagcagaagg
     9601 gctcgctcga gaacctcgcg ctcatgccgt ccgaggccgc cggcgccgcg ctcgaggagg
     9661 gccaggtccg cgtcgcgatg cgcgccgccg gactcaactt ccgcgacgtc ctgatcgccc
     9721 tcgggatcta ccccggcgag gcgccactcg gcagcgaggg agccggcgtc gtcgtggagg
     9781 tggggccggg cgtcgagcgc ctcgccccgg gcgaccgtgt gatgggcttc ttcggcgagg
     9841 cgttcggctc gcacgcgatc ggcgagcacc agatgctcgt gccgatcccc gccggctggt
     9901 cctacgccca ggccgccgcg gtcccgaccg tcttcctgac cgcgtactac gcgctgttcg
     9961 acctggccga gatcgagcgc ggtgagcggt tgctcgtcca cggcgcagcg ggcggcgtgg
    10021 gcatggcggc cctgcagctc gccgagcacg ccggggccga ggtctacgcc accgcccacc
    10081 ccggcaagtg ggacacgctg cgcgagctgg gcgtggacga cgaccacatc gcctcctcgc
    10141 gcgacctcga cttccgcgag cgcttcctgt cggccaccgg tggcgagggc atgaacgtgg
    10201 tcctcgactc gctggccaac gagttcgtcg acgcctcgct cgagctgctg ccccgcgggg
    10261 gtcgcttcgt cgagatcggc aaggccgacg tccgcgaggc cgacgaggtc gcacgcgagc
    10321 acgagggcgt gaggtaccgg gcgttcgatc tgatcgaggc cgggcccgag cgcatccagg
    10381 agatgctggt cgaggtggtc gagctgttcg agcgcggcgt gctccgccac ccgccgatct
    10441 cggcctggga cgtgcgccgc ggcgccgagg cgttccgcca catgcgcgag gcccgccacg
    10501 tcggcaagat cgtgctgagc atccccggcg cgctggagcc ggagggcacc gtgctcgtca
    10561 ccggcggcac cggcggcctc ggagcgctcc tggcccgcca cctcgcggag gagcacggcg
    10621 tccgccgcct cgtcctgacg agccgccgcg gcgccgaggc cgaaggggcg aaggaggtcg
    10681 cggccgcgct cgaggagctc ggctgcgagg ccgagctcgc cgcgtgcgac gtggccgacc
    10741 gcgaccagct cgaggccctg ctcgcacgta ttcccgacga gcacccgctg accgcggtgg
    10801 tccacgccgc gggcgtgctc gacgacgggc tgatcgggtc gctcgacgcg gagagcgtgc
    10861 gcagggtcat ggcccccaag gtcgacggcg cgctcaacct gcaccgcctc acgcgggacc
    10921 tcgacctcgc ccagttcgtc ctcttctcgt cgatcgccgc gaccgtcggc tcgcccgggc
    10981 aggcgaacta ctcggccgcg aacgcgttcc tcgacgcgct ggcgcaccac cgcgtcaccc
    11041 gcggcctggc cgcgacggcc atggcgtggg gcccctggga gcgcggcatg gccgccggcc
    11101 aggacgaggc cgaccgggcg cgcgcgcagc gcatggggat gacgccgctc gcggacgagg
    11161 agggcctcgc gctcttcgac gcgtcgctca ccgccggcga ggccgtgctg gtccccgtcc
    11221 gcctcgacac cgcggccctg cgcgcggcgg gcgagggcct attgccgccg atcctgagcg
    11281 ggctgatccg cgtccgctcg gaaggcggag gcgacgcgac gggctcgctg cagaggcgcc
    11341 tctccggtgt accggagtcg aagtggtccg cgatcgtcct cgacatggtg cgggaccagg
    11401 tcgcgtccgt gcgcggcctc gagtcgggtg acgcggtgaa gccggacctc gcgttcaagg
    11461 agatgggctt cgactcgctc gccgcggtcg agctgcgcaa cggcctcacg cgatccacgg
    11521 ggatccggct gcccgcgacg ctcgtgttcg accacccgac cccctccgcg gtcgccgagc
    11581 acgtgctcgg ccggctaccg cgtccggacg cggggccctc gatcgaggac gaattcgacc
    11641 gaatcgagcg actgctgcac gatgcgtcga tggacgagcg gacccggagc cggatggagg
    11701 cacgggtgcg cactttcaac tctcgcgttc agagcctgct gatggggtcg agcgacgggg
    11761 atgacggcga tcgcgacgag gacctcgggt cggtttcgaa cgaagaggtg ttcgccctga
    11821 ttgacaagga gattggagcc tcatgagcga cgagcagcgc ctgaacgagt acctgcgcaa
    11881 ggccaccggc gaactccgcg ccgctcgccg gcgcgtgcac gagctcgaac agcgtgatca
    11941 cgagccgatc gcgatcgtcg gcatgagctg ccgctatccg cggaccgtcc gctcgccgaa
    12001 ggagctctgg gagctcgtcg cgaccggggc cgaggccgtc ggcccgtatc cggacgaccg
    12061 cgggtgggac atcgaccatc tctacgatcc cgatcccgac cacatcggca ccgtcttcgc
    12121 ccgtgagggc gcgttcctgg aaggcatcgc cgagttcgac gccgagttct tcggcatcgg
    12181 cccgcgcgag gcggtcgcga tgaacccgca gcagcggatc atgctcgaga ccgcgtggga
    12241 ggcgttcgag gacgccggca tcgatcccgc gtcgttgcgc ggcagccgca ccggagtgtt
    12301 cgccggtgtc ttccacgacg actacgtcag cggcccggcc cgccacccgt ccagcgccga
    12361 cgccgagcgt ttcgcctcga cgggcgagcc gacgtccctg gtgtccggtc gcgtggccta
    12421 cgccttcgga ctcgagggac cgacgatgtc ggtcgacacg gcctgctcct cgtcgctcgt
    12481 cgccatgcat ctcgcgtgcc acgccctgcg cgaccgcgag tgctccctcg cgctcgcggg
    12541 cggcgtcacg accatgtcca cgacgatgat gctgcgcgcc ttcagccgtc agagggcgct
    12601 ctctccggat ggtcgctgca aggcgttcgg cgcggacgcc gacggtacgg gcttctcgga
    12661 gggcgggggc ctcctggtgc tcgagcgcct ctccgacgcg cgccggcggg gccaccgggt
    12721 cctggccgtg atgcgcggta gcgcggtcaa ccaggacggc gcgtctaacg ggctgaccgc
    12781 gccgaacggg ccttcgcagg agcgcctcat ccgcgacgcg ctcgcggatg cggggctgtc
    12841 gcccgccgac gtggacgtgg tcgaggggca cggcacggga acgacgctcg gcgacccgat
    12901 cgaggcccag gcgctgctcg cgacctatgg ccaggagcgc tcgaatggac cgctctacct
    12961 cggctcgatc aagtcgaaca tcggccacac ctcggcgggg gccggcgtcg ccggcgttat
    13021 caagatggtc gaggcgatgc gccacgacct gctgccgcgc tcgctctact gcgaggagcc
    13081 ctcgacgcac gtcgactggt cggccggcgc ggtcgagctg ctgcgcgagc cggtcgagtg
    13141 gccggcgggc gagcgcccgc gccgggcggg cgtctcctcc ttcggggtct cgggcaccaa
    13201 cgcccacgtg atcatcgagg aggcgccggt cgaggaggag gctccggccg agcgctccga
    13261 gctgccggcg ctgccgttcc tcgtctcggg ccgtagccag gcggccctgc gcgcgcaggc
    13321 cgagcgcctg cgcgactggc tcaccgagcg ccccgagctc gagccgctcg acgtggcctt
    13381 caccctggcc acggggcggg ccaagctcca gcaccgggcg gcactgatcg cctctgaccg
    13441 cgacgatctc gtggctgggc tcgaggcgct cgcgggcggc ggttcgaggc ccgggatggc
    13501 ggtcggcgag gagcggccgg gcaagaccgc gttccagttc acgggtcagg gcgcgcagtg
    13561 gccggggatg ggcgcggagt tctgcgagcg gttcccggcc ttcgccgagg cgctcgacgc
    13621 ggtctgcggc gagctcgatc cccatctcga gcggccgctg aaggagctca tcttcgccgc
    13681 ggaggactcg ccggaggccg agctcctggg atcgacggag ttcacgcagg cggccctgtt
    13741 cgcgatcgag gtggcgctct atcggctgtt cgagtcgtgg ggagtgcggc ctgactatct
    13801 gatcggccac tcgattggcg agctggtggc ggcgcacgtg gccggcgtgc tctcgctgtc
    13861 cgacgcctgc acgctcgtcg cggcgcgcgg gcgactgatg ggggcgctcc ccgaggacgg
    13921 ggcgatgctc gcgatcgcgg cgtcggagca cgaggtcgcc ggcacgcttg ccgagcttgg
    13981 cggcgccgtc tcgctggcgg cgatcaacag tccgctgtcg gttgtcgtct cgggtgagcg
    14041 cgaggcggtc gaatcgtgcg aggcgacctg gagcgagctg gatcgcaagg tctcgcgcct
    14101 tcgcgtcagc cacgcgttcc actcacacct gatggagccg atgctcgacg agctccggaa
    14161 ggtggccgag gggctgacct tcgaggcgcc gcaggtcgag gtcatctcga acctcaccgg
    14221 cggtctggtc tcggacgagc tgcgcgaccc cgactactgg gtccggcatg tgcgcgagac
    14281 cgttcgcttc gccgacggcg tggccacgct cacgcggctc ggcgtgacgc gcttcgtcga
    14341 gctcggcccg gacggtgtgc tgtcggcgat ggcacgccag tcggtcagcg ccgagctcgg
    14401 caagcgtgcc gtgttcgcgc cggcgatgag ctcgcgtcgc gagccgctgg cgactgttct
    14461 cggctgcctc ggcgccctgc acgccgccgg cggcgaggtc gattggccgg ccttcttcga
    14521 ggggagcggc ggccgcctgg tcgatctgcc gacgtacgcc ttccagcgcg agcgctactg
    14581 gctcgaggcg gcgggcgagg tcggcgaggc ctcggccggc gacctcacgg cagccggcca
    14641 gctcgcggtg gagcacccgc tgctcggcgc tgcggtgcga ctcgcggccg ggcagggcgg
    14701 ttggctgttc acgggtcggc tgtcgtccca gacgcatccg tggctgttcg accacacggt
    14761 gacgggcgtc tcggtgatgc cggggaccgg cttcgtcgag ctggcgctcg ccgcggcgca
    14821 gcgcgtggga gccgaggggg tcgaggagct gacgctggtg gcgccgctcg ccttcgacga
    14881 ggagggcgcg gtgcggctgc aggtcgcggt cagcgagccg gatgacgatg gccggcgggc
    14941 gatcggcgtc tactcgtcgc ctaccgccga cgcgagcgac gagaacggct cgacggactg
    15001 ggtgctgcac gccacggggc ggctcggcgc gccgggcgcg acggcccccg agagcgccga
    15061 ggcgttccgc gcggagtggc cgcccgcgga cgcccaggag gtggacgtcg agtcgttcta
    15121 cgaccgcgcg gcggaggcgg gttatgacta cgggcccgcc ttccagacgt tgcggcgtgc
    15181 ctaccgcaac ggcgaggcct cgtttgccga ggtcggtctc ggcgagacgg tccagagcga
    15241 cgccggccgc ttccgcgtcc atccggccct gttcgacggg gcgttgcacg cgatgctgct
    15301 cgaggcgctc gaaggggccg gaaccgacgg cgctccgcgc gtgccgttcg cgttctcccg
    15361 ggtgtcgctg caccgcgagg gtgccgcggc gctgcgcgtg cgcatcgaga cgaccacgga
    15421 cgacgccagc gacgccacca ccctcaggct gctggcggtc gacgagtcgg gggcgccggt
    15481 gctggtggtg gatgcgatcg acgtgcggcc gttcgaccag gcgactctcg cggaggccgc
    15541 ccggaaggcg cgcccgcagg cgggcgaggg ctcgctgctc actgtcgagt ggacgggggt
    15601 cgaggcggcg ggctcggacg atccgtggct cgaagccgcg ctgctcggtg acgacgacgg
    15661 agtgctcgag gggtcggggc tgagcctgga tccctatgcg gacttcgagg cgctcgagca
    15721 ggcgctcgcc ggcgggacga ccgctcccga gatggtgctg atgcgccccg gcgcggcgga
    15781 tggtccgctg gcgggctcgg tcgccgagtt cacgcagcgg gcgctggagc tgttgcaggc
    15841 ttggctcgac tccgaggcgc tggccgacac gcggctggta tttgtcacag atcgcgcgct
    15901 ggcggtggcg ccgggtgagt cgcccaacct gtcgcaggcc gcgctcgtcg gtctgttgcg
    15961 cagcgcgcgg tcggagcatc cgggccgttt cgcgctggtc gacctggacg gaagcgacga
    16021 gtcggtggtg gccctgttcg gggcgttgac gagcgacgag ccggagctgg cggtgcgcaa
    16081 cggcgtgctc tacgcgccgc ggctggctcg cgccgctggg ctcaccatgc ccgcgggcga
    16141 gggcgcctgg cgggtcgcga gcgagcgcaa gggctcactc gaggacctcg caatcgtcac
    16201 ctcgaacgct ggggatgacg cgctcgcgga gggcgaggtc cgggtcgcga tgcgtgctgc
    16261 cggcctgaac ttccgcgatg tgctgatcgc gctcgggatg tatccgtacg aggccccgct
    16321 cggcagcgag ggggcgggga tcgtggtcga ggtcggcccc ggcgtcgagc gcctttcgcc
    16381 gggcgaccgg gtgatgggct tcgtcggcga ggcgttcggt tcgcacgcgg tcaccgacgc
    16441 gcgattcctg gtgccgctcc cggacggttg gtcgttcgtc gaggcggcgt cggtgccgac
    16501 cgtcttcctg accgcctact acgcgctgtt cgacctggcc gagctccagt cgggtgagcg
    16561 gttgctggtg cacggcgctg ccggcggcgt cgggatggcg gcgttgcagc tggccgagca
    16621 catcggcgcg gaggtgttcg cgaccgccca caagcgcaag tggggcacgc tgcgcgatct
    16681 gggagtggag cgctcgcaca tcggctcctc gcgcgagctc acgttccgcg agcgcttcct
    16741 caaggccacc gacggcgagg gagtggacgt ggtcctcgac tcgctggcca acgagttcgt
    16801 cgacgcctcg ctcgagctgc tgccgcgtgg cgggcgattc atcgagatcg ggaaggccga
    16861 catccgcgag cccgacacgg tcgccgccga gcaccagggc gtgcgctacc gggccttcga
    16921 cctgtccgac gtcgagcccg agcgggtcca ggagctgctg ctcgaggtgg tccggctctt
    16981 cgaggcgggg gcgctgcgcc acctgccgat ccagagctgg gacgtgcgca gcgcaccgga
    17041 agcgttccgg tacatgcgcg aggcgcgcca cgtcggcaag atcgtgctca ccgtccctca
    17101 gcagccggac cccgagggca cgatcctgat caccggcgcg accggagggc tcggcgagct
    17161 cgtcgcgcgt cacctcgccg accaccacgg cgcccggcac ctactgctcg ccagccgccg
    17221 cggcgaagag gccggcggcg cccgcgagct cgtggacgcg ctcgcggagc acggctgcga
    17281 agccaagctc gcggcctgcg acgtcgctga ccgcggccag ctcgagaagc tgctcgccga
    17341 gatcccggcc gaccacccgc tgacggcggt gatccacgcc gccggcacgc tcgacgacgg
    17401 ggtgatcggc tcgctcgacg ccgagcggct gcggggagtg atggcgccga aggtcgacgg
    17461 ggcgatcaac ctgcacgagc tgaccgccga cggcgagctc gcgcagttcg tcctgttctc
    17521 ctcgatcgcc ggcacgctcg gcacgccggg ccaggccaac tacgcggccg ccaacgcgtt
    17581 cctcgacgcg ctcgcccacg accggagcgc ccaggggctc gccgccaagt cgctcgcatg
    17641 gggcgcctgg gaacgcggca tggccgcggg cctcagcgac accgaccgcc agcggggaga
    17701 gcgcatcggc atcggagcgc tctccgacga ggacggtctc gcgctgctcg acgccgcgaa
    17761 cgcgaccgcc gagccgcaac tcgcgccgct cgccatcgac accaaggccc tgagcgcgca
    17821 tgccggctcc gggctgctgc cgccgatgct gcagggactg gtgcgggagt ccgcccggga
    17881 ggaccgcggc gacgcgcaga actcgctggg gcagcggctg gccgctgctc caaagtccga
    17941 gtgggagtcg atcgtcgtcg atctcgtccg gagcgaggtg gcgagcgtgc tcgggcacag
    18001 ctcctcggac gcggtcgatc cggagcgcga gttcaaggag ctcggattcg attcgctggc
    18061 ggccgtggag ctcggcaacc gcctgaccga tgcgaccggc ctggcgatcc agccgagcgt
    18121 caccttcgac ctgcagacgc cacatgcact ggccgcatac ctcgccgagc gactgcgtga
    18181 cgaggctccc gatgtacccg agggagctcc tgctgcgcag gagtctccgg ggacaccggt
    18241 gactcagggt gcgccggcgg acggcgcgcc cgtgaagcgc ccgagcacgg ccggcacgct
    18301 gaccgcactg ctgcgcggtg cgcacgagca gggcacgctc ggcgacttcg ttcccgccgc
    18361 catggcgaca tcgaagttcg ctcgcgcctt ccggtcgccg gcggagctcg agcatctgcc
    18421 gtcgctcgtg ccgctctcgc gcggcgcggg cacgcggctg atatgcgtcc cgtcgttcct
    18481 cgccggatcc ggaccgcacc agttctcgcg gctggcgagc catctggacg gcgtccggac
    18541 gatgtccgcc ctctcgttgc cgggcttccg cacgggtgag ctcgttccgg gtacctggga
    18601 tgccctcatc gaggcgctgg ccgcttcggt tcgcgaggcc gcgaccgacg acccgttcgt
    18661 gctcgtcagc tactcccatg gcgggggccc agcgcactcg ctggctggaa agctcgagga
    18721 gcagggcctg gcgccggcgg ggctggtgat gatcgacacc tacgcgcccg agtcggagga
    18781 agagatgcgc cagctcttcc ggaaggtggt ggagaccatc ttcgagaacg accacccgct
    18841 gatcgaaggc tcggtcgacg acggcaacct gctggccatg ggcgcctact tccggctggc
    18901 cgaggaatgg gaggcgaccc ccatcgagac gccgcatctg ctcatccgtg cgaccgagcc
    18961 gctcggggat gcgtacgagg gcggccgcct gccctggtgg cagctcccgt ccgaggtgac
    19021 cgaggtcacg ggcaaccact tcggactgat cgaccagtca gccggggaga ccgctcaggt
    19081 catcgacgcc tgggtcaggg agaagacggg cgagccggca cccacgcgcg acgccgaggt
    19141 ggcccgtgcg gggtagagcg ccgcgatctc ccgacgccgg gtgatcgggc gattggtcga
    19201 tcggagcgct cgcgcgtagg gtgcgccacg gagagaagag gaggtcacag atggcggcca
    19261 cacccaagtc agcccccccg ccccgcaacg gcgcgggcgt caccgatccg atcgccgacg
    19321 ccgtcaacag gggcgactac gacctcttcg cgcaagcgct cgcggaggac gtcgtcttcc
    19381 gctcgccggt atcccgcttc aggttccgag ggccggagat cacatccgcg ctgttcgagc
    19441 ggctggtgaa gcaatcggac cccgaccggt ggggagtgca ggactcctgg agccttgcac
    19501 cggacgcgca cgccatggcc ctcaccacga acgtccgggg tcatcagctc gacctgttga
    19561 tcgtgacgcg actgaacgag cgtcatcaga tcagccaggt gacggcgtac gcgcggccga
    19621 tggcgtcgat cgccatcttt ccggcgttcg tctaccccca cctcgtcgag cgattccgcg
    19681 ggccgggacg agcggcggtg gtccggacgg tgttccggcc gcttccgcgg ctcctcaagg
    19741 tcctcgtctc ggcgggactc ggcttcgggc gcccgccgca agcggagttc gaggaggccg
    19801 agctcccgac gcgggaagaa ccgccggcgg cgccgtcggc cggcccgagt tcggcgtcgg
    19861 acggcggcca atcgtcaggt gggccgcgac cgtcgtcgga cggcgcgcaa tccccaccgg
    19921 ccggtccccg atcgccattt cgccggcttt gactgcgcat cgcaggtcgt gtgagctacg
    19981 gagtagcagt cccgcaaagt gctgtcgatc cttgggagca acgtgatgga gaaccgagtc
    20041 cacgacgtcg tagtcatggg gggcgggcag gcaggcctat cgctcacatt gcagctcaag
    20101 cagcgtgatc ccgacatcga catcctcgtc gtcgagcgga gcgagcaccc accgcccagc
    20161 gcggcccaca aggtcggaga gtcgacggtg gagggcgggg cctactactt cagcaagatc
    20221 ctcggattaa agccgtatct cgacaaggag cagctccgca agtccgggct ccggtactac
    20281 ttctccgacg gtcgcaacac cgacctcgcc cgccgcgtcg agctcggcaa cagcaggcag
    20341 ctcccgttcg ccacctacca gctcgaccgt ggcaccttcg aggcgaagct gggctcgctc
    20401 gtgagcgaag ccggggccga gttcctcgac aaggccgtgg tccgggacgt caccctcggc
    20461 gagggtgacg acccgcaccg cattcagatc aagcgcgacg gtgtcgagag tgaggtccaa
    20521 gcccgctggg tcgtcgacgc gacagggcga ttccagttcc tgaagcggaa gctcgacctg
    20581 gccaagcagg ttgaccacaa gatgaacgcg tcgtggttcc gcctccccgc gaaggtcgtc
    20641 gtcacggatt ggtcggacga tcccgactgg cagaaccgag tggggaagtt cgtgctctcc
    20701 gacggaaccc cggcgcagct ccgctcgctg agcacggtgc acctggtggg ccacgggtgg
    20761 tggctgtggc tgatcccgct ggccggcgac gtcacgagcg tcggcatcgt ggtcgacgac
    20821 agggtcttcc cgatctccga gatcaactcg ctggagaagt cgatggcgtg gatagaggag
    20881 cacgagccgc aggcctacga ggccatcgct ccgcacatcg acgacgtgtt ggacttcaag
    20941 ttcctacgtc actactcgca tagctgcgag cgcattctct cgcccgagcg gtggtgcatc
    21001 accggcgtcg ccggagcctt ccacgacccc ctcttctccg tcggggccga catcatctgc
    21061 tggaacaaca cgatcatcac cgacgtcatc gcaagagatc acgccggcga ggacatcggc
    21121 agtcgcgtcc agctgtacaa cagcatcttc ctggatcagt acgtgggtcc gatgtacgcg
    21181 ctgttcgagg acacctacct ctacatgggc aacccgcagg tgttctcggt gtgggtccac
    21241 tggagcacct gctggtactg gtccatcctc gcgagcatca tcacgcacga caagatcacg
    21301 gacctcgaga cgctctctac cctccacgac gaggtcaagc gctcgatcga cctgtggcac
    21361 gtgatgcaga agttcttcac cgagtggtgg gaagcggccg gtgacgacta ctcgcacacg
    21421 gaccacttca tcccgctctt caatcagccg tggatcaatc gcctccaggc cgacctgctc
    21481 gccgactggg attccgagga gttcacgcag aagcttcggg agaacctcga gaacctcgag
    21541 gcggcggtgg catatgtctt ctggatggcc gtcgagggac ttcccgaccc gccggagcga
    21601 cgcccgatcg acccctacaa gatcaccatg gatcgcgatc gatgggaggc ggacgggttg
    21661 ttcgacaccg acggggtcac cccgtcgaat cccgtcgacc tcgacgccga gttcggtggc
    21721 tatggctggt tcagccgcga tcgagccggt gcggccgagg aggccgcact gagtcagtcg
    21781 agctagcgga ccgagccggc tacgccgccg ctgggtcgag ggccggctcg gcggcggcgg
    21841 gagctcggac agctccctcc ctctcccgcc gggcgacctg catccgcgcg acgacgagcc
    21901 gctggatctc ggcggtgccc tcgaacagct ggtagacctt ggcgtcgcgg aagaacttct
    21961 cgagcgggca atcggccgtc tgcgcgtagg ggccgaccag atccatcagg gtcgtcgtcg
    22021 cccacacggc cacgttcccg cccttgagct tcgacatcga gccctggccg ccggtcatcg
    22081 ggatgccgtt gcgacccatc caggcggcac gctggaccag gagccgcgcc gcgtcgatct
    22141 cggtggcgac gtcggcgatg atctgctgga cctcttgacg gcgtagcggc ggctcgccgg
    22201 cggtcgagct ctcgtcgaga gcggtccgga tcgggttcaa ctcgccacct tcggcgagga
    22261 agtcgagcgt ccagtcatag gccgcttgag cgatgccgac ggccgcggcg cccaccatcg
    22321 gccgggtgac ctcgaaggtg gccatggcgt tcgagctctg gcccctcgac gcacccgacc
    22381 gggcccgttc gagcctctcg ttgagccgat ccatgccccc gagcaagttc tcgaccggca
    22441 cgcggcagtc ctcgaggacc acctcggccg tgtgactggc tcggatgccg agcttggact
    22501 ccttcttccc ctggcgcagg ccgggcgtcc ccttggggat gacgaaggag gcctgcccct
    22561 cctggccgag ctcgggatcg acgcttgcca cgatcacgtg gacgtcggcg atgccgccat
    22621 tggttatgaa gaccttggtg ccgttgagga tccactcgtc gccgtcgagc tcggcgcgcg
    22681 tcttcaggct cttgaggtcc gagccggctt cgggttcggt gaccccgtag gcgccgagct
    22741 tcggctcctc ggcagtgccg aggcattcgg gcatccactg ggctatctgc tcaggagcgc
    22801 cggcggcaac gatgccgctg gtggcaagca ccgacccggt gatcgcgagg gctatccccg
    22861 cgcagcccca gtgaaactcc tcggggagga tcacgaggca ttgacccgtc gggtccgcgc
    22921 ccatctgccc catgcgctcg agtccgttga ggccccactc gtgtgacgcc ctgatcactt
    22981 cccagggcgt cgactcctcg gcgtcgtgct tggccgctgc cggtcgaatc acctcgcgcg
    23041 cgaaccgacg gcagagatcg cgaaactcga gggcctcgtc cgagagagcg aagttcatgc
    23101 gggccgcacc gcgatcgtca tggcaaatac ctccgagcgc ggacgctcgc agaaacggga
    23161 ttcgtcggtc aaatccagtt atgcgctggc ggcaggctgc gtaacactcc gctctgcatg
    23221 cgcgccatcg acatcaccgc gccgccgctc gagccggagg agctgaccga ccgcctccgg
    23281 cgcgcgccgt ggcggcgtta cgtcacgctc ggcgacagcc tcgtcgaggg catcggcgac
    23341 ccggtcgagg gataccccga gatgggctgg gccgaggcca cgggccggac gttcaaggaa
    23401 ctgcatgcgg acttcgagtt cctcaacctc ggcaagcgct ttctcacggc ggaccgggtg
    23461 cgcatgaccc agctccagcc ggcgctcgac ttcgagccgg acctcgcggc ggtgctcgcc
    23521 ggcggcaacg acctcggcga tccgttcgac cccgggggga tcgagcgcga gctcgacagg
    23581 atggtcgccg cgctgagcgc caccggagcg accgtgatgg tgtggtcggt gctcaacatg
    23641 atgagctgcg ggcgctaccc cgagccgatg gtggatctgc tggcgcctcg cctcgcggcc
    23701 caccgcgaca tctcgctgcg ggtcgccgag cgctacgaca cggtcttctt cgactgcttc
    23761 aaccacccga tgtgcgccga cccgtcgatc ctcagcgagg acctgctcca catcaacatg
    23821 cgcggccagg cgctgctcac cgagatgacg ctgcagggcc tggcgtcgct cgtcccggcc
    23881 cggacggccg cctgagcggg ccgccgccta gtacgggacg aagtcgtcct tgtgcacgcc
    23941 gcaggccggg cagacccagt ccatggggat gtcctcgaac ggcgtgccgg gcgggatccc
    24001 gccgtcggga tcaccctcgg cggggtcgta gatgaacccg cattcctgac agatccacag
    24061 ttgctgcgtc tgggtggtca tgttgctcaa gttacgcgga tcgctcgggt agacgaaatc
    24121 cgacttcagg ccacgagcgg atcacactcg cccaggaaag ccaggatgct cccgctgctc
    24181 tcgtccaagc gctcgaagat gccgtgcccg gccccttcgt atgcctcgaa ccgggccgtc
    24241 gggatccggc gagcgagctc ctcgccggcc gcgataggca cgatccgatc ctcggtgccg
    24301 tgatgcacca gaaccggcat cctcaggcgg gacagccgcg accagctcga atgacccgcg
    24361 gcggcgagga cctgcatgac caagccgcgt tgactggagg gcacctcgag cgacgccgca
    24421 acggcggccc atgcctcgtc ctctggccgg caattcgcca cgtagtcctg tgacgcggcc
    24481 atggagagga agtctcgtac tcgctgctct ggatcgctcg ccggccggaa cgaccgcaac
    24541 acggtcccga agatcggcag gatccccctc cgcggcacca gatccaggcg cggcaacaga
    24601 ctcagatcca ttcgcgggag tccgcccgtg gtgcacagga gcaccagggc tcccacccgc
    24661 tcggggaatt cgagcgcgat ctcctgcgcg atcatcccac cgaacgaagc gcccccgatg
    24721 tgggcgcgct cgatccccag ctgatccagc accgcgaccg cgtcggccgc catcgtgctc
    24781 gtcgtgaagg ggagccatgg cgtgcccgaa cgcccgcagc cgcggttgtc cacggcgatc
    24841 acgtcgtggc cgtcgccaag caggcgcctc acggcgggcc cccagaggcg accgttgccg
    24901 cccagtccca tcaagagcag gacgggcgct gcgccttcgc gagccaaatc tggttccggt
    24961 gagaacagct cgtagtagat gcgcgtgccg tcatctgcgg tcacgtacga ctcgaccggg
    25021 cgattcctct gatcagatac gcgttgggat tgagacccga gcgacgtcgt gctcatgtgc
    25081 tgtgctccaa ttccgggttg ttgcggtggt ctgtcgcccc tatcagcgcg agcaggtggt
    25141 ggcgggagaa gatgtttccc aggcgcttcg ccgcccgggg atcgccggcg acggtcagcg
    25201 cgccagtggc agccgccgag gcagccgtga ccttcccgga ggtgacggcc tcccaggtct
    25261 cctcgtcggt ggtcagggtg acggcgggat cgtgcgcaaa cccgtcgcgg aggcggatcg
    25321 cgccgtcgtc gatggtgaag tagaaggcca agcggccgac gaggtactgg taggtctcgc
    25381 tgacgcccct tgcggcctct cggtcagcga agttcgccat cgccatcgcc gcccagcgcg
    25441 gccgaaagct gtccgtcggg tcacgctcgc cgagacgcct ggcgccccag ccgatcagcc
    25501 cggtcatcgc ctcggccaga tcgcgaccgt ccgcggttag cccgtacacc tgccgcgcgg
    25561 caggcggcgg gaggtcttcc cgggcgatga cgccatcgcg ttcgagggcg cgtagccgtt
    25621 cggtgaggag gttgcggctg attcctggca ggccgtcgat cagatcggtg aaccgtcgcg
    25681 ggccgggcac gagctcgcgg acgatcagca gggtccaccg atcgccggcg acgtcgaggg
    25741 cgcgcgccag gccgcagtac tgctgatagc ggtgctcagg catctgggcc cctcatgcgt
    25801 ccggtaacgg cgttgtggcc ttcgcctcgt cgaacactcg gttgtccagc acccgcgcaa
    25861 gcggccgtgg gagccaccag ttccgccgtc cgagcagctg catggtcgaa ggcacgagca
    25921 cgagccgaac gaccgtcgcg tcgagggcga ttccgaccgc gagccccaac ccgaactccc
    25981 tgacgctcgt gaggcccgcg ctcgcgaaca cgcagaagag ggccaccatg atcaccgccg
    26041 ctcccgtgat ggggcgcgcg ctcatcgcca gtccctgggc gatcgcggag ctgggatcgc
    26101 cggtgcgctc gtactgctcg cggatacggg acatcaggaa gatctcgtaa tccatcgaga
    26161 gggcgaacac gcccgtcatg atgggcagca agccgaaggt gttcaccgag ccctcggact
    26221 tgaagccgag gaatccgtcc agccatcccc actggaacac cgccaccacc gcgccgtatg
    26281 cggccccaac ggtcagcagg ttcaggatca ccgccttgag cggcaggacc agcgaacgca
    26341 gcagcacggt cagcagcacg aacgagaagg ccaggccgag cacggcgacc ttccagagat
    26401 ttccgaagat ctgatcctgg aagtcgatgc tgtaggccgt gacgccgccc acctgcgccg
    26461 tgacgcccgg ggacacgggc tcgtcgaact ccgaccggag ccgtctgacc agttcctccg
    26521 tggacggcga gctcccgtcg gagcgggggg ttgccgtcag caggacggac cgccctccct
    26581 cggcagcctt cggaggcgcg acgccggcga cctcggggtc gcgggcgatc gtcgcccgcg
    26641 cggaggcgac cgcctctcgc tcgaccggct gcggccgctc cgattgcagg accacctgga
    26701 cagggctcgt cgccccgggt ccctgttccg ccgcggcccg gtcgtagccc acccgggtgt
    26761 cgttgtcccg cgggagctgg ccgacgagat gctcacccgg gtcgaggctc agcatcggca
    26821 gcgcgaggac gagcagcgtc agggtcgcag cagtcgcgaa caggatcggc ctaccgagca
    26881 cgacgcgggt ccacctctcc cagaacgacg gtcgtgagcc ggtcgccggt ttggcgcttc
    26941 gccgcgataa cagcgggagc cggcgctgtt gcacgagccg gttgccgagc agccgcatca
    27001 ggacgggcag gagcaccgcg gagagcagca ccgcggttat caccgtgaac aggatcgcgc
    27061 ccaccatgga gcgcatgccg gcgttgtcga cgatcgctat ggcgccgatc gcgaccacga
    27121 ccgtcaggcc ggagaagacg atggcgacgc ccgaggtgga cagcgcccga gagatcgcgg
    27181 catcgcggtc gcgcccggct cgaatctcct ctcggaagcg cacgaggatg aacatcgagt
    27241 aatccaccgc cagggcgatg ccgaacagcg acgccgagtt cgtggcgaag accgacacga
    27301 ggaactcctg cgacgccagg tagatcgccg cccccgtgat taggaccgcg gcgataccca
    27361 ggacgaccgg caacgcagcg gcagccaggg agccgaagat cgtcaggagg atcagaagca
    27421 cgatcggcaa gccgatcgtc tcggcccgct cgagctgcgt cgtcgcctcc tcctggatgg
    27481 ccacgtccag ggcgccgcgg ccgatcacgt gcgagctcac ggaccgcccc gcgccgtcca
    27541 ccccacggag gcgatcgtgg atgtccttcg ccacctcgga ctcccgatcc tgactcgcca
    27601 gcacgccgac cgggagaatg gcggtgcgtc cgcgttcggc cgccgcccgt gcgcggccgc
    27661 gcgtagagcc ggggagcgtc gcctctggca cgtcctggat cgaacggagc agggcgtcga
    27721 ccgcctgcac tcgatccgcg gctttcgagc ccggcttcgg cgcgagcacg accgcgagct
    27781 gagcgcgctc gtcgaagtcg cgcgcgagcg cctcacgaac ctgctcggac tcagatccag
    27841 gaaccctgtt gccaccggca acggtgtcct cggactggcg agctgcgagc ggcgcggcca
    27901 caactacgag aaccagccac gcggcgagga cagcccagcg ccaccgccgg acccccgccg
    27961 cgaggcgctc catgagggcg ctcatcgatt ccgtctctcg gtggacatcg ctctacgcat
    28021 tcggctcggg gattccgtcg agtgctgcgc agccttcggg gattccgtcg agtgctgcgc
    28081 agcctttgtt cattgaaact gccccaggta tcgtaattga accaaacgtc gtacggacag
    28141 cgagccaaaa gggagtcaga tggcagagac caccacagca gcgggagctc tcacaaacga
    28201 gtgggtcgag gagttcgggg agcgttactt cgcggcctgg gactcacacg atcccgaccg
    28261 gctgctcgag ctgatgaccg aggacgtcgt ctacgaggac tcggcctggc cgatgcccat
    28321 gaacggtcac gccgaggtgc gtgagttcct cgagttcgtc taccgggcct ttcccgacat
    28381 aaggttcgag cacgaggtgc cgctgatcgc cgcggacgag ccaagggccg cgttcccctg
    28441 cgtcggatgg gcgacgaact ccggtccgat cgagccaccc gggataccgg cgacgggaga
    28501 gaagctcgac tggaagggca tcgacatcat cgacttccgc gacggcatgg tctcccggct
    28561 gcggatcctc tacaacccgg cgcccggctg gctgcagctg ggcctgctgc agggtgtgga
    28621 gctggacggc ggagaaacag ctcgccgcac gctcggcgag accgagtagg tctcagaagc
    28681 gatcgcccgc gctgccgccg gcggaccgta cggtctagcc ggcggcccgc ggcgcgggcc
    28741 gtaccgcgat cgtcatgcgt acgagcgcgc acaggcgctc gtcgtcgtcg ctgatctcga
    28801 cgtcccagac ccaggtggtc ctaccgcggt gtcgcggacg ggcgaccgcg ttcaggtgcc
    28861 cttcgctgat cgggcgaagg aaggtcgtgt cgttcgactg cccccatgtg gtcatcccgt
    28921 cgttgatcac cgccgcgtag gtcgcggccg agcagatgcc ctcggccatg gtcgcgaaga
    28981 cccccccgtg gacgaagccg acgggtccct tgtggtggtt ctccaccggg atccgggagc
    29041 gggcctcgtc cggtcccagg tcgagccatt ccgtgccgag gtgggcgtcg aaggcgtccg
    29101 cggccaacgt atcgaggaga cgctcgcggg ccggattgcc gaccgcaagc gtggaccatt
    29161 gctcgtggcg atcgtcgggc gtctggttca tttattaaac ggcagagtac ctacacaaaa
    29221 actcagctgg agacggcgtc cggcaggcgg gcgaccccct gcagctcggc gagctcgtcg
    29281 ttccagtcga tccgatcgcc cttccagtcg atcgggtcgg actccgtgct
//
LOCUS       DASVPL010000014        14237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_157571, whole genome shotgun sequence.
ACCESSION   DASVPL010000014 DASVPL010000000
VERSION     DASVPL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157571"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VF517_00650"
     CDS             <1..217
                     /locus_tag="VF517_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051472.1"
                     /translation="RDLVLGAGALGTTGGERRRWLQAAVVADVADALAAVAGHRNGYL
                     PTHGAIMLGLTAATGTALGIAALASSD"
     gene            217..1059
                     /gene="rfbD"
                     /locus_tag="VF517_00655"
     CDS             217..1059
                     /gene="rfbD"
                     /locus_tag="VF517_00655"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX8051473.1"
                     /translation="MRVVITGAAGMLGRDVVRAAQAENHEVVALARDELDLLDAEAVR
                     AAIAAERPGAVINCAAWTDVDGAEADEAAATRLNGDAAGALAAAAAEAGAAIVHPSTD
                     YVFDGSGTRPYVESDPTSPINAYGRSKLAGEHAVAAANVRHFVVRTSWLFGANGRNFV
                     ETMLGLASAGGPVLVVRDQVGCPTYTGHLAEALVRLVDGESYGLHHIAGGGECSWFEF
                     ANEIFERAATETRAMSCTSDEFPRPAPRPAYSVLRTERDYGLELPPWQDGLASYLAER
                     AVTR"
     gene            1056..2057
                     /gene="rfbB"
                     /locus_tag="VF517_00660"
     CDS             1056..2057
                     /gene="rfbB"
                     /locus_tag="VF517_00660"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX8051474.1"
                     /translation="MRLLVTGAAGFIGSTYARIVVEEHGDDVVVLDKLTYAGRRENLA
                     GIEDRVEFVEGGIEDRDLVGELVASCDAVVNFAAESHVDRSIADQDVFARTHVIGTGV
                     LLDAVREHGVSRYLQVSTDEVYGSIEEGSFTETSPLNPSSPYSATKAAGDLLVSAHVH
                     TYGIEAVICRGSNNYGPRQYPEKLIPLMILNALHGDALPVYGDGRQVRNWLFVEDFAR
                     AIDLTLRRGAPGEVYNVGGPDEAENIDLVRRLIELTGADESLIEHVTDRPGHDRRYSL
                     SSDKVRALGWEPLTSLDGGLERTVDWYRDNEWWWAPIRSGEYADYYARQYGRRLGTS"
     gene            complement(2054..2353)
                     /locus_tag="VF517_00665"
     CDS             complement(2054..2353)
                     /locus_tag="VF517_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051475.1"
                     /translation="MDDWTAVVLWAALALEAAVATSAARLGMDDRKDHHRRATLARRL
                     HEQGVLPEDLGSLLIRLNTERKHAVHEGRQPDLRGRDWDSVLDSIERAVAAADGS"
     gene            2455..3693
                     /locus_tag="VF517_00670"
     CDS             2455..3693
                     /locus_tag="VF517_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8051476.1"
                     /translation="MGRDSCVVVACIASLAIAWAGAPAVAAPAEPTDAAAETTTAPAE
                     TTTAPAETTTAPAEPTKDELERQKLIAERGKLDAERRKADRDNSWSARTLPALLTFIV
                     GLGTLLYAVKKGTDELQASRAESKRQHDLDAQARKDAEAESKRQHDLDAQARKDADAK
                     RFDERFITAMEASASKDAAARRGGAVVLDSLIRDTDRAAAAQGMNLLLALLQDLETRE
                     NDASTRRVLRQTLERRLRAPDASKVATEFDFRYLAVPGIDLSGVVLNGDVDLAFSDLS
                     GGTLRKADLSGTKGWHLVLVGTRLRRARLAHVEWAEADARTAKFHNAILTKANLWKAD
                     LRGADFYRASLRDANLRGAKLAGAQFNLANVAGAQFRQAELDAAAIASLAAAVGWEEA
                     EFDAPVRAEIEKAASDTGEQ"
     gene            3810..4547
                     /locus_tag="VF517_00675"
     CDS             3810..4547
                     /locus_tag="VF517_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8051477.1"
                     /translation="MPAALEINGLTKRYDDGTAALSDFDLTIPEGKFFGLLGPNGAGK
                     TTLISAVCNLIRITAGDVCVFGEPAHSLQARRWVGLAEQDINLDRFLTVEETLIYHGG
                     YFGMSKDDACARAYEMIGVFDLRAKVDVRTPKLSGGMRRRLLLARALMHRPRLVILDE
                     PTAGVDFELRQELWRYIRKLHEQGTTILLTTHYLEEAETLCEEIALIRAGRLIARDTG
                     AGLLERYEATSLADVYVKAMGAGVAPS"
     gene            4705..5478
                     /locus_tag="VF517_00680"
     CDS             4705..5478
                     /locus_tag="VF517_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8051478.1"
                     /translation="MQPYSGTAGMVWLIRRETLRVSKLWTQTVLAPIVSSGLFILVFG
                     FSLSSRIREFDGIPYREFIVPGLITMAMVQAAYSNNSSSVFQARSDRYINDVLSAPMK
                     AWQMNFGFNVGGVIRALAIGVGLGAVAVPVTGAPIEQPLVLIPAVLLGLVLFGALGTI
                     VGIFAETFDHHTFVNNIVILPLVFVGGVFYSVDLLPSPWHELTHFNPIFYLVNAVRYG
                     FLGTSDVSVALCLGVTAGLAIPAYLWSQWLFTSGRKLKA"
     gene            5514..6179
                     /locus_tag="VF517_00685"
     CDS             5514..6179
                     /locus_tag="VF517_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX8051479.1"
                     /translation="MSELGRLQQRVVDCRRCPRLVEWRERVAREKRASFAAETYWGRP
                     IPGFGDPAARVYVLGLAPAAHGGNRTGRIFTGDRSGDWLFASMHRTGFASQAEAFHVL
                     DGLRLDGIYVGAAVRCAPPANKPLPAERDNCLPYAEEELRLLADVRVVVCLGQFAWDA
                     ATRLLGVRPKPRFGHLAEHAVPEGPAMLASFHPSQQNTFTGKLTEPMLDAVFERAREL
                     AAA"
     gene            complement(6202..7035)
                     /locus_tag="VF517_00690"
     CDS             complement(6202..7035)
                     /locus_tag="VF517_00690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8051480.1"
                     /translation="MPDLPGYRIEGVAGRGGWSVVYRAVQLSLDRPVALKLIAPELAG
                     DPGFRDRFRRECELAASIDHPNVMPIYDAGEAGDSPFVAMRWVDGTDLRSLVAEHPLH
                     PERAVAIVVQVAMALDAAHARGLVHRDVKPGNVMIEPRAGGEHAYLADFGLAKPIDAD
                     PGLTEAGRWLGTVDFAAPEQIRGGPPDARSDVYALGGLLLHALTGAVPFPRADDAAKM
                     RAHLHEAPRPLPPPLARYEPVVRRALAKQPAERFASAAELGRALVAAGAAASPAPHAM
                     P"
     gene            complement(7038..7757)
                     /locus_tag="VF517_00695"
     CDS             complement(7038..7757)
                     /locus_tag="VF517_00695"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8051481.1"
                     /translation="MTRLLAALVAAGLALAAAPAPPVAQEPAECTAIVIDPGHDSRAN
                     PETEPIGPGSKKRKIKDGGGTRGVVSGTPEHVVTLAISKRLRKTLTARGYCVTMTRDR
                     ATGLSMGNVARARIANRARAALFVRVHADGSTNRSQHGSAMLYPAAHAGWTDDVLPES
                     RTAAKLLQRELVARIGSRDNGIVPRGDLTGFNWSDVPVVLAEIGFMTNPAEDRRLNDA
                     EYQQRAALGLARGIERFAPAD"
     gene            7834..10737
                     /locus_tag="VF517_00700"
     CDS             7834..10737
                     /locus_tag="VF517_00700"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX8051482.1"
                     /translation="MSKRLTIGLACAAAALATAPAALAAPHRTLNLSQANTSASPVTW
                     DGTLHDGTNSMYNSAAGAPCGESTDTYCDDTLLNVSLPAGFRGGVQVDIDNYRPNPLT
                     DFDLYVYESDAAGHALRLLENDGTFGEVLVGAGLPNGFPESYAFPGKSGYYLVRVVYF
                     DAPVASQYSASAQVTNLVARADKPPDVDAPAGLQEYLASNPTLGFKSHSEPHIAQSPI
                     DPDVLVAGSKQYYRDRDSLKEYEFKIGTYVSFDRGVTWTDLGQLDVCPIAQAPQSSYP
                     ASNTCYPDDDPSKGGTGAEDVKENQEDMEFDDRGSGDLAEEYTTSDIWVQFDDEGSAY
                     VMVLDHPPFPIQSDPLGLGTEENGWGMTLHKWESVSSDDLRTGKTWGPRVPINFYADE
                     VRERVFLDDKNTMAINNVGPDHDGKPGTIVTCWGRGAALVKQQIVCERSTDGGKTFPG
                     EPIPVSEPQNVGIGVFVAPDTKDEHTFHLMWNYYINPNSPLNQIYYTKSIDDGQTWLP
                     PRPITAPFTPLPSTYPGQQFRQLSIPMMAVAPNGDLYATYSHYRPAPDVATDEDGEQA
                     DVVFIKSTDGGLTWSDPAVVNQDKSNADQFQPQIAISPQGEVNIAYFDRRNDKRTVVG
                     GQVTHAGNYYVDEYLSRSTDGGATWQDTRLSHEMSDPEQNAPVSPSGLFFGDYQGLVA
                     DDCFTIPFYQDAHLALDRTRDDGFDAGFPRSEYQETFAWRVPNAKQGPACTKPVVEGP
                     SPPVTPEGLPTVSVTAPRLASDVGTSNKFNVRVNASAANIDFYQLEYRRRGSTKWKRL
                     SSKLLNADYRFRGSYSQTYAFRARAIDLSGREGPWSGEAVSVIPHDDRRAKGRPRYSR
                     GWARAKGTTAFKRTLTTTSRKGSSMRINFRGNRVYLIGRKGPRGGRALVILNGKRKQV
                     SFYKSKTAERQVVYSASIAGSRTSTLQVVALGRKGSKKSKGTRVEIDAVGYRG"
     gene            10783..>14237
                     /locus_tag="VF517_00705"
     CDS             10783..>14237
                     /locus_tag="VF517_00705"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8051483.1"
                     /translation="MSSMALDPLATFTPQVRDWFGRAFAEPTEAQRQAWPAIARGEDV
                     LLSAPTGSGKTLAAFLWGLDRLVAEPAPEGERHTRLVYVSPLKALSYDIERNLRAPLK
                     GIGGDVTVALRTGDTPQKERQAMLRNPADVLITTPESLYLMLTSRARDTLAGVEWVIV
                     DEIHAVAQTKRGAHLAITLERLERQAGQRVQRIGLSATQRPLEEVGRFLSGAGRECTV
                     VDAGVRKPLDVRIQVPVEDMAEPDVQEDVLNLESGDTATRRSIWPAIYPELLDLIQQH
                     QSTIVFVNARRGAERLAMRLNELAAERAELAIGRSSEGGSLSEPASVPPRSSARGPAP
                     VAPLEIARAHHGSLAREERLIVEEQLKSGALPCLVATSSLELGIDMGAVDLVLQVESP
                     KSVTRGLQRIGRAGHGVGETSKGRIFPKFRADLLEATVVVKRMRAGEIESTVVPRNPL
                     DVLAQQIVAMVAGDEELSVPEMHDLIRRAYPYSDLSREQLDNVLDMLDGRYPSEQFGD
                     LRPRIVWDRVADTIRARQGARQLAVTNAGTIPDRGMYGVHLPDGRRVGELDEEMVYEA
                     RPGQTFLLGASTWRIEEITRDRVIVSPAPGTPGAIPFWRGDGVGRPVELGRAIGEFSR
                     WAVDQEPAELAKSHDLDERAAKNLVQFLREQREATRVIPSDRTVVIERFRDEIGDWRL
                     CILSPFGGRVHSAWGLALSARIRDEAGLEADAIWSDDGIIVHLPDADEPPGADLVLID
                     PDELEDLVVRELGSSALFGARFRENAARALLIPRAYPGKRTPLWQQRLKAQSLLEVAK
                     HYGRFPIVLETYRECLRDVLDLPALTDLLRKLQRRELSLVEVETQRASPFASSLLFDY
                     VATYMYEGDTPNAERRAAALSLDRDLLRELLGQEELRELIDPDALAEVEADLQRISER
                     MRAANADALHDVLRAVGDLTTVEAQARSLEAVSAKRMLDQLRGERRAIAMRIGGEERW
                     IASEDAGLYRDALGAVPPGGLPETFLESVDEPLARLVRRYARTHGPFTTAEIGGRYGI
                     DPAPVLRELERAGELVRGELRPEGTEREWCDPEVLRRLRRASLASLRKEVEPAEHRAL
                     ARLLPEWQGVDGAPPGGAGVDRLREALVPLQGIALAPEVWERDVLPRRVGAYSPTWMD
                     SLCAAG"
BASE COUNT         2086 a         5138 c         5101 g         1912 t
ORIGIN      
        1 acgcgacctg gtcctcgggg cgggcgcgct cggcacgaca ggcggcgagc ggcgccgctg
       61 gctccaggcg gccgtggtcg ccgacgtggc cgacgccctc gcggccgtcg cgggccaccg
      121 caacggctac ctcccgacgc acggcgcgat catgctcggg ctgacggcgg ccacgggcac
      181 cgcgctcggc atcgcggcac tcgcctcctc cgactgatgc gggtggtcat cacaggcgcg
      241 gccggcatgc tcggccggga cgtcgtgcgc gcggctcagg ccgagaacca cgaggtggtg
      301 gcgcttgcgc gcgacgagct cgacctcctc gacgcggagg ccgtgcgcgc ggccatcgcc
      361 gccgagcgcc ccggcgcggt gatcaactgc gccgcctgga ccgacgtgga cggcgccgag
      421 gccgacgagg cggccgccac ccgcctgaac ggcgacgccg ccggcgccct ggccgccgcc
      481 gcggccgagg ccggcgcggc catcgttcac ccctcgaccg actacgtgtt cgacggctcc
      541 ggcacgcggc cgtacgtcga gtccgacccg acgagcccga tcaacgccta cgggcgctcc
      601 aagctcgccg gcgagcacgc cgtggccgcc gcgaacgtgc gccacttcgt ggtgcgcacc
      661 tcgtggctgt tcggcgcgaa cggccgcaac ttcgtcgaga cgatgctcgg gctcgcgagc
      721 gccggcggcc cggtgctcgt cgtgcgcgac caggtcggct gccccaccta caccggtcac
      781 ctcgccgagg cgctggtgcg gctggtcgac ggcgagtcgt acggcctcca ccacatcgcc
      841 ggcggcggcg agtgctcgtg gttcgagttc gccaacgaga tcttcgagcg cgcggccacc
      901 gagacccgcg ccatgtcctg cacgagcgac gagttcccgc gtcccgcgcc gcgccccgcg
      961 tactcggtgc tgcgcaccga gcgcgactac ggcctcgagc tgccgccctg gcaggacggc
     1021 ctggcgagct acctcgccga gcgagcggtc acgcggtgag gctgctcgtc acgggcgcgg
     1081 ccggcttcat cggctcgacc tacgcgcgga tcgtggtgga ggagcacggc gacgacgtgg
     1141 tcgtgctgga caagctcacc tacgccgggc gccgcgagaa cctggccggc attgaggaca
     1201 gggtcgaatt cgtcgagggc ggaatcgagg accgcgacct cgtgggcgag ctggtcgcga
     1261 gctgcgatgc ggtcgtgaat ttcgccgccg agtcgcacgt cgaccgctcg atcgccgacc
     1321 aggacgtctt cgcgcgcacg cacgtgatcg ggaccggggt gctgctcgac gcggtgcgcg
     1381 agcacggcgt gtcgcgctac ctgcaggtct cgaccgacga ggtctacgga tcgatcgagg
     1441 agggctcgtt caccgagacc tcgccgctca acccgtcctc gccctacagc gcgacgaagg
     1501 cggccggcga cctgctcgtc tccgcgcacg tgcacaccta cggaatcgag gcggtgatct
     1561 gccgcggctc gaacaactac ggcccgcgcc agtacccgga gaagctgatc ccgctgatga
     1621 tcctgaacgc gctgcacggc gacgcgctgc cggtgtacgg cgacgggcgt caggtgcgca
     1681 actggctgtt cgtggaggac ttcgcgcgcg ctatcgacct gacgcttcgc aggggcgcgc
     1741 ccggcgaggt ctacaacgtc ggcggtcccg acgaggccga gaacatcgac ctcgtgcggc
     1801 ggctgatcga gctaaccggg gccgacgagt cgctgatcga gcacgtcacc gaccgccccg
     1861 gtcacgaccg ccgctactcg ctctcgtccg acaaggtccg cgccctcggc tgggagccgc
     1921 tgacgagcct cgacggcggg ctcgagcgga cggtcgactg gtaccgcgac aacgagtggt
     1981 ggtgggcgcc gatccgcagc ggcgagtacg ccgactacta cgcccgccag tacggccgcc
     2041 ggctcggcac gagctaggag ccgtcggcgg cggcgaccgc ccgctcgatc gagtccagca
     2101 cggagtccca gtcccggccc cgcaggtcgg gctgcctccc ctcgtggacc gcgtgcttgc
     2161 gctcggtgtt caggcggatc aggaggctgc cgagatcctc gggcagcacg ccctgctcgt
     2221 gcagccggcg cgcgagcgtg gccctccggt gatggtcctt gcggtcgtcc atgccgagcc
     2281 gcgccgccga ggtcgcgacc gccgcctcga gcgccagagc cgcccacagg accaccgcgg
     2341 tccagtcgtc cacggcgtac gccgcgcgcg cccgtcccag gtgatcggcg gcgagggacc
     2401 gtggtttggg ggatgacccg ctggcgctca tacgcatatg atcgcgactg agtgatgggg
     2461 agggacagtt gcgtggtggt cgcgtgcatc gcatcgctcg cgatcgcctg ggctggcgcg
     2521 cccgcggtag ccgctcccgc cgagcccacg gacgctgccg ccgagaccac taccgctccc
     2581 gccgagacca caaccgctcc cgccgagacc acaaccgctc ccgccgagcc cacgaaggac
     2641 gagctcgagc ggcagaagct catcgccgag cgcgggaagc tcgacgccga acggcgcaag
     2701 gcggatcgcg acaacagctg gagcgcccgc accctgccgg ccctcctgac gttcatcgtc
     2761 gggctgggca cgctcctcta cgccgtcaag aagggaaccg acgaactgca ggcctcgcgc
     2821 gccgagtcga agcgccagca cgacctcgac gcccaggcgc gcaaggacgc ggaggcggag
     2881 tcgaaacgcc agcacgacct cgacgcccag gcgcgcaagg acgcggacgc gaagcgcttc
     2941 gacgagcgct tcatcaccgc gatggaggct tccgcgtcga aggacgcggc ggcgcggcgg
     3001 ggcggggcgg tggtgctcga cagcctgatt cgcgacaccg atcgggccgc cgcggcccag
     3061 ggcatgaacc tcctgctcgc gctgctccag gacctcgaga cgcgcgagaa cgatgcaagc
     3121 acgcgccgcg tgctgagaca gacgctcgag cggcgactcc gagcgccgga cgcgagcaag
     3181 gtggccaccg agttcgactt ccgctacctc gcggtgccgg ggatcgacct gagcggcgtg
     3241 gtgctcaacg gcgacgtcga cctcgcgttc tccgacctgt ccggcggcac gctgcggaag
     3301 gccgacctct cgggcacgaa gggatggcac ctcgtcctgg tcggcacccg cctgcgacgc
     3361 gcgcggctcg cgcacgtgga atgggcggag gccgacgcac gcacggcgaa gttccacaac
     3421 gcgatcctga ccaaggccaa cctgtggaag gcggacctgc gcggcgcgga cttctacagg
     3481 gccagcctgc gtgacgccaa cctgcgcggg gccaagctcg ccggcgcgca gttcaacttg
     3541 gcgaacgtcg ccggcgccca gttccgccag gccgagctcg acgccgcggc catcgcgagc
     3601 ctcgcggcgg cggtgggctg ggaggaggcg gagttcgacg ccccggttcg agcggagatc
     3661 gagaaggccg cgagcgacac gggcgagcag tagaggaaat cggacgccgg gcgctctgag
     3721 cgcagccccg tggggttgcg cgaccggccc ggcgtcctgg tcgagatgat aggcgctgat
     3781 gcgctgctcc gcaccactag cctcggaggg tgcccgccgc cctcgagatc aacggactga
     3841 cgaagcgcta cgacgacggc accgccgcgc tgagcgactt cgacctgacg atcccagagg
     3901 gcaagttctt cggtctgctc ggaccgaacg gggccggcaa gaccacgctg atcagcgcgg
     3961 tctgcaacct catcaggatc acggccgggg acgtctgcgt gttcggcgag cccgcccact
     4021 ccctccaggc gcgccgctgg gtcgggctgg cggagcagga catcaacctc gatcgcttcc
     4081 tcacggtcga ggagacgctg atctaccacg gcggctactt cgggatgtcg aaggacgacg
     4141 cgtgcgctcg cgcttacgag atgatcggcg tgttcgacct gcgcgcgaag gtggacgtgc
     4201 gcacgcccaa gctctccggc ggcatgcgcc ggcgcctgct gctggcccgg gcactgatgc
     4261 accggccgcg gctcgtgatc ctcgacgagc ccaccgccgg ggtggacttc gagctgcgcc
     4321 aggagctgtg gcgctacatc cgcaagctcc acgagcaggg cacgacgatc ctgctcacca
     4381 cgcactacct ggaggaggcc gagacgctct gcgaggagat cgcgctgatc cgcgccggcc
     4441 gcctgatcgc ccgcgacacg ggcgcggggc tgctcgagcg ctacgaggcg accagcctcg
     4501 cggacgtcta tgtcaaggcg atgggcgcgg gggtggcgcc gtcctaatgc aggtagcgcg
     4561 ggtcggagaa ggcggtcgcg cgccggcggc gcgccgtccg ggtgcgccgg atggcgccca
     4621 ccacgagggc accggcgaat gcgagggcga tcgtctccat gctcgtaaaa cgcgccggcg
     4681 cgggcgaggt tgagggttag cggcgtgcag ccctactcgg gaaccgcggg gatggtctgg
     4741 ctgatccggc gcgagacgct gcgcgtctcc aagctctgga cccagacggt cctagcgccg
     4801 atcgtctcct cgggcctgtt catcctcgtc ttcggcttct cgctgagcag ccgcatccgc
     4861 gagttcgacg ggatccccta ccgcgagttc atcgtgcccg gcctgatcac gatggcgatg
     4921 gtccaggccg cctactcgaa caactcgtcg tcggtcttcc aggcgcgctc ggaccgctac
     4981 atcaacgacg tcctgtcggc cccgatgaag gcgtggcaga tgaacttcgg cttcaacgtc
     5041 ggcggggtca tccgcgcgct ggcgatcggg gtgggactcg gcgcggtcgc ggtccccgtc
     5101 accggcgccc cgatcgagca gccgctcgtg ctcatccccg ccgtgctgct ggggctcgtg
     5161 ctgttcggtg cgctgggcac gatcgtcggg atcttcgccg agaccttcga ccaccacacg
     5221 ttcgtgaaca acatcgtgat cctgccgctc gtgttcgtcg gtggggtctt ctactcggtg
     5281 gacctgctgc cgtcaccctg gcacgagctg acccacttca acccgatctt ctacctcgtg
     5341 aacgcggtca ggtatggctt tctgggtacc agtgacgtta gtgtcgccct ttgcctcggg
     5401 gtgactgccg gactcgcaat cccggcctac ctctggtccc aatggctatt cacgagcggc
     5461 cgcaagctga aggcttagac ccactcctcg agctccgcac ggggctggat cccatgtccg
     5521 agctcggacg cctccagcag cgcgtggtcg attgccgccg ctgcccgcgg ctggtcgagt
     5581 ggcgcgagcg ggtggcacgg gagaagcggg cctcgttcgc cgccgagacc tactgggggc
     5641 ggccaatccc ggggttcggc gaccccgccg cgcgcgtgta cgtgctgggc ctcgccccgg
     5701 cggcgcacgg cggcaacagg accgggcgga tcttcacggg cgaccgctcg ggcgactggt
     5761 tgttcgcgtc gatgcaccgc acgggcttcg ccagccaggc ggaggcgttc cacgtgctcg
     5821 acggcctgcg cctcgacggc atctatgtgg gcgccgcggt gcgctgcgcg ccgccggcca
     5881 acaagcctct gccggccgag cgcgacaact gcctgccgta cgccgaggag gagctccggc
     5941 tgctggcgga cgtgcgcgtg gtcgtctgcc tcggtcagtt cgcctgggac gcggccacgc
     6001 ggctcctcgg cgtgcggccg aagcccaggt tcggccacct tgccgagcac gccgtccccg
     6061 aggggccggc gatgctcgcc tcgttccatc cgagccagca gaacacattc acggggaagc
     6121 tgaccgagcc gatgctcgac gccgtgttcg agcgcgcccg cgagctggcg gcggcctagc
     6181 ccggggtcga gcggccgctc gtcacggcat cgcgtgcggc gccgggctcg cagcggcccc
     6241 tgccgccacc agggcccggc cgagctcggc cgccgacgcg aaccgctcgg cgggctgctt
     6301 cgcgagcgca cggcggacca cgggctcgta gcgcgcgagc ggcggcggga gcggccgcgg
     6361 cgcctcgtgg aggtgggcgc gcatcttcgc ggcgtcgtcc gcgcgcggga acgggaccgc
     6421 gccggtgagg gcgtggagca ggaggccgcc gagcgcgtag acgtcggagc gcgcgtcggg
     6481 cgggccgccg cggatctgct cgggggcggc gaagtccacg gtcccgagcc agcgcccggc
     6541 ctcggtgagg cccgggtccg cgtcgatcgg cttcgcgagg ccgaagtcgg cgaggtacgc
     6601 gtgctcgccg cccgcgcgcg gctcgatcat cacgtttccg ggcttcacgt cgcgatgcac
     6661 gagcccgcgc gcgtgcgcgg cgtcgagcgc catcgcgacc tggaccacga tcgcgaccgc
     6721 gcgctcgggg tgcagcgggt gctcggcgac caggctgcgc aggtccgtgc cgtcgaccca
     6781 ccgcatcgcc acgaagggcg agtcgccggc ctcgcccgcg tcgtagatcg gcatgacgtt
     6841 cgggtggtcg atcgaggcgg ccagctcgca ttcgcggcgg aagcggtcgc ggaacccggg
     6901 gtcgccggcc agctccgggg cgatcagctt cagcgccacc ggcctgtcga gcgagagctg
     6961 caccgcgcgg tacacgacgc tccacccgcc gcgtccggca accccctcga tccgatagcc
     7021 gggcaggtcc ggcacggcta gtccgccggc gcgaagcgct cgatgccgcg cgccagcccg
     7081 agcgcggcgc gctgctggta ctcggcgtcg ttcagccggc ggtcctcggc cgggttcgtc
     7141 atgaagccga tctcggcgag cacgaccggc acgtcggacc agttgaagcc ggtgagatcg
     7201 ccgcggggga cgatgccgtt gtcgcggctg ccgatccgcg ccaccagctc gcgctggagc
     7261 agcttcgccg ccgtgcgcga ctcgggcagg acgtcgtcgg tccagcccgc gtgcgcggcc
     7321 ggatagagca tcgcgctccc gtgctgcgac cggttcgtcg agccgtcggc gtggacgcgc
     7381 acgaacagcg cggcccgcgc gcggttggcg atccgcgccc gcgcgacgtt gcccatgctg
     7441 agcccggtgg cgcggtcgcg ggtcatcgtc acgcagtagc cgcgggcggt gagcgtcttg
     7501 cgcagccgct tcgagatagc gagcgtgacc acgtgctcgg gggtcccgct cacgaccccg
     7561 cgcgtgccac cgccgtcctt gatcttgcgc ttcttcgagc cgggcccgat cggctccgtc
     7621 tccgggttcg cgcgcgagtc gtgccccggg tcgatcacga tcgccgtgca ctccgccggc
     7681 tcctgcgcga cgggcggtgc gggggccgcg gcgagcgcga gccccgccgc gaccagcgcg
     7741 gcgagcaggc gtgtcatgcg accgcctcga tccggtgacc gcttatctcg tacttctcag
     7801 ccaattggag taacgtgcca tgccggggtg gggatgagca aacgtctgac gatagggctg
     7861 gcctgtgccg cagcggcgct ggccaccgcg cctgcggcgc tggccgcgcc gcatcgcacg
     7921 ctgaacctga gccaggcgaa cacctcggcc agcccggtca cctgggacgg caccctccac
     7981 gacggcacca acagcatgta caacagcgcc gcgggcgcgc cgtgcggcga gagcacggac
     8041 acctactgcg acgacacgct gctgaacgtc agcctgcccg ccgggttccg cggcggcgtg
     8101 caggtggaca tcgacaacta ccggcccaac ccgctcacgg acttcgacct ctacgtctac
     8161 gagagcgacg ccgcggggca cgccctgcgc ctgctcgaga acgacggcac gttcggcgag
     8221 gtgctggtcg gcgcgggcct tccgaacggc ttccccgaga gctacgcgtt cccgggcaag
     8281 agcggctact acctcgttcg cgtcgtctac ttcgacgctc cggtcgcgtc gcagtactcg
     8341 gcctcggccc aggtcaccaa cctggtcgcg cgcgccgaca agccgccgga cgtcgatgca
     8401 ccggccgggc tccaggagta cctcgccagc aacccgacgc tcggcttcaa gtcgcattcc
     8461 gagccccaca tcgcgcagag cccgatcgat ccggacgtgc tggtcgcggg gtcgaagcag
     8521 tactaccgcg accgcgactc gctcaaggaa tacgagttca agatcggcac gtacgtctcg
     8581 ttcgaccgcg gggtcacgtg gaccgacctg ggccagctcg acgtgtgccc gatcgcgcag
     8641 gcgccgcagt cgtcctaccc ggccagcaac acgtgctacc cggacgacga tcccagcaag
     8701 ggcggcaccg gcgccgagga cgtcaaggag aaccaggagg acatggagtt cgacgatcgc
     8761 ggctccggcg acctcgccga ggagtacacg acctcggaca tctgggtcca gttcgacgac
     8821 gagggcagcg cgtacgtgat ggtgctcgac cacccgccgt tcccgatcca gtccgacccg
     8881 ctcgggctcg ggacggagga gaacggctgg gggatgaccc tccacaagtg ggagtcggtc
     8941 tcctcggacg acctgcgcac cgggaagaca tggggcccgc gcgtgccgat caacttctac
     9001 gcggacgagg ttcgcgagcg cgtcttcctc gacgacaaga acacgatggc gatcaacaac
     9061 gtcgggccgg accacgacgg caagcccggc acgatcgtga cctgctgggg ccgcggcgcc
     9121 gcgctcgtca agcagcagat cgtctgcgag cgctcgaccg acggcgggaa gaccttcccc
     9181 ggcgagccga tcccggtctc cgagccccag aacgtcggga tcggcgtgtt cgtcgcgccc
     9241 gacaccaagg acgagcacac cttccacctg atgtggaact actacatcaa cccgaactcg
     9301 ccgctcaacc agatctacta cacgaagagc atcgacgacg ggcagacctg gctgccgccg
     9361 cgcccgatca cggcgccgtt cacgccgctg ccgagcacct accccgggca gcagttcagg
     9421 cagctctcga taccgatgat ggccgttgcc ccgaacggcg acctgtacgc cacctactcc
     9481 cactaccgcc cggcgccgga cgtggccacc gacgaggacg gagagcaggc cgacgtcgtg
     9541 ttcatcaagt cgaccgacgg gggcctgacg tggagcgacc ccgccgtggt caaccaggac
     9601 aagagcaatg ccgaccagtt ccagccgcag atcgcgatca gcccgcaggg cgaggtcaac
     9661 atcgcctact tcgaccgccg caacgacaag cggaccgtgg tcggcgggca ggtcacgcac
     9721 gcgggcaact actacgtgga cgagtacctg tcgcggtcga ccgacggcgg cgccacctgg
     9781 caggacacgc gcctctcgca cgagatgtcg gaccccgagc agaacgcgcc ggtctcgccg
     9841 tccgggctct tcttcggcga ctaccagggg ctcgtcgccg acgactgctt cacgatcccg
     9901 ttctaccagg acgcgcacct cgcgctcgat cgcacccgcg acgacgggtt cgacgcgggc
     9961 ttcccgcgct cggagtacca ggagacgttc gcgtggcggg tgccgaacgc gaagcagggc
    10021 ccggcgtgca cgaagccggt cgtcgagggg ccgagcccgc cggtgacgcc cgagggcctg
    10081 ccgacggtga gcgtcacggc gccgcggctc gccagcgacg tcggcacgag caacaagttc
    10141 aacgtgcgcg tgaacgcctc ggccgcgaac atcgacttct accaactcga gtaccgccgc
    10201 cgcgggagca cgaagtggaa gcggttgagc tcgaagctgc tgaacgcgga ctaccgcttc
    10261 cggggcagct acagccagac ctacgcgttc cgcgcgcggg cgatcgacct gtcgggccgc
    10321 gagggcccgt ggtcgggcga ggccgtcagc gtgatcccgc acgacgaccg gcgcgccaag
    10381 ggccgcccgc gctactcgcg cggctgggcg cgagccaagg gcacgaccgc gttcaagcgc
    10441 acgctcacca ccacgagccg caagggctcc tcgatgcgga tcaacttccg cggcaaccgc
    10501 gtctacctga tcggtcgcaa gggaccgcgc ggcggccgcg cgctggtgat cctcaacggc
    10561 aagcgcaagc aggtgagctt ctacaagtcg aagaccgccg agcgccaggt ggtctacagc
    10621 gccagcatcg cggggtcgcg gacgagcacg ctccaggttg tcgcgctggg ccgcaagggc
    10681 agcaagaagt ccaagggcac acgcgtcgag atcgacgccg tcggctaccg cggctagggc
    10741 catccaggcg ccgcgggcgc gcgtactcgg gaaggtacgc tgatgagttc gatggctctc
    10801 gatccactcg cgacgttcac cccgcaggtt cgtgattggt tcggtcgcgc gttcgcggag
    10861 ccgaccgagg cgcagcgcca ggcctggccg gcaatcgctc gcggggagga cgtcctgctg
    10921 tcggccccga ccggctcggg caagacgctc gcggcgttcc tctggggcct cgaccgcctg
    10981 gtcgccgagc ccgcacccga gggcgagcgg cacacccggc tcgtgtacgt ctccccgctg
    11041 aaggcgctct cctacgacat cgagcgcaac ctgcgcgcgc cgctgaaggg gatcggcggc
    11101 gacgtgaccg tcgcgctgcg cacgggcgac acgccgcaga aggagcgcca ggcgatgctc
    11161 cgcaacccgg cggacgtcct gatcaccacg ccggagtcgc tctacctgat gctcacctcg
    11221 cgcgcgcggg acacgctcgc cggcgtcgag tgggtgatcg tggacgagat ccacgccgtc
    11281 gcgcagacca agcgcggcgc gcacctcgcg atcacgctgg agcgcctcga gcgccaggcc
    11341 gggcagcggg ttcagcggat cggcctgtcg gccacccagc gcccgctcga ggaggtcggg
    11401 cgcttcctct ccggcgcggg gcgcgagtgc acggtggtcg acgccggcgt gcgcaagccg
    11461 ctcgacgtcc ggatccaggt gccggtcgag gacatggccg agcccgacgt ccaggaggac
    11521 gtgctcaacc tggagtcggg cgacaccgcc acgcgccgct cgatctggcc cgcgatctac
    11581 cccgagctgc tcgacctgat ccagcagcac cagtccacga tcgtgttcgt gaacgcccgc
    11641 cgcggcgccg agcggctggc gatgcggctg aacgagctgg ctgccgagcg tgccgagctg
    11701 gccatcgggc ggagctccga ggggggctct ctgagtgagc ccgcgagcgt acccccgagg
    11761 agctctgcac gggggccagc ccccgtcgcg ccgctcgaga tcgcccgcgc ccaccacggg
    11821 tcgctggccc gcgaggagcg gctgatcgtg gaggagcagc tcaagtcggg cgcgctcccg
    11881 tgcctcgtgg cgacctcctc gctcgagctg ggcatcgaca tgggcgcggt ggacctcgtg
    11941 ctccaggtcg agtcgccgaa gtcggtcacg cgcgggctcc agcggatcgg ccgcgccggg
    12001 cacggcgtgg gcgagacctc gaagggccgg atcttcccga agttccgcgc cgacctgctc
    12061 gaggccacgg tggtcgtcaa gcggatgcgc gcgggcgaga tcgagagcac ggtggtaccg
    12121 cgcaacccgc tcgacgtgct cgcgcagcag atcgtggcga tggtggcggg cgacgaggaa
    12181 ctgagcgtgc ccgagatgca cgacctgatc cgccgcgcct atccctactc ggacctctcg
    12241 cgcgagcagc tcgacaatgt gctcgacatg ctcgacggcc gctacccgtc cgagcagttc
    12301 ggcgacctgc gcccgcgcat cgtgtgggac cgggtggccg acacgatccg cgcgcggcag
    12361 ggtgcgcgcc agctcgcggt cacgaacgcg ggcacgatcc cggaccgggg catgtacggc
    12421 gtgcacctcc cggacggccg ccgagtcggt gagctcgacg aggagatggt ctacgaggcg
    12481 cgcccggggc agacgttcct gctcggcgcc tcgacctggc ggatcgagga gatcacgcgc
    12541 gaccgggtga tcgtctcgcc cgcgccgggc acgcccggcg cgatcccgtt ctggcgcggc
    12601 gacggcgtcg ggcgcccggt ggagctcggc cgcgcgatcg gtgagttcag ccgctgggcg
    12661 gtggaccagg agccggccga gctggcgaag agccacgacc tcgacgagcg cgccgcgaag
    12721 aacctcgtcc agttcctgcg cgagcagcgc gaggccacgc gggtgatccc gtcggaccgg
    12781 accgtggtga tcgagcgctt ccgcgacgag atcggcgact ggcggctgtg catcctctcg
    12841 ccgttcggcg ggcgggtcca ctcggcctgg ggcctggcgc tgagcgcgcg gatccgcgac
    12901 gaggcgggcc tcgaggccga cgcgatctgg tcggacgacg gcatcatcgt ccacctcccc
    12961 gacgcggacg agccgcccgg cgcggacctc gtcctgatcg accccgacga gctcgaggac
    13021 ctggtcgtgc gcgagctcgg ctcctcggcg ctgttcggcg cgcgcttccg cgagaacgcc
    13081 gcccgcgcgc tcttgatccc gcgcgcgtat cccggcaagc gcacgccgct ctggcagcag
    13141 cgcctgaagg cgcagtcgct gctcgaggtc gccaagcact acggccgctt cccgatcgtg
    13201 ctcgagacct accgcgagtg cctgcgcgac gtgctcgacc tcccggcgct caccgacctg
    13261 ctgcgcaagc tccagcgccg cgagctgtcg ctcgtcgagg tcgagaccca gcgcgcgtcg
    13321 ccgttcgcct cgtcgctgtt gttcgactac gtcgcgacgt acatgtacga gggcgacacg
    13381 cccaacgccg agcgccgcgc ggccgcgctc tcgctcgacc gcgacctgct gcgcgagctg
    13441 ctcgggcagg aggagctgcg cgagctgatc gacccggacg cgctggcgga ggtcgaggcc
    13501 gacctccagc gcatcagcga gcgcatgcga gccgcgaacg cggacgcgct ccacgacgtc
    13561 ctgcgcgcgg tcggcgatct gaccaccgtc gaggcgcagg cgcgctcgct cgaggccgtg
    13621 tcggcgaagc gcatgctcga ccagctccgt ggcgaacgcc gcgcgatcgc gatgcggatc
    13681 ggcggcgagg agcgctggat cgcctcggag gacgccggcc tgtaccgcga cgcgctcggg
    13741 gcggtgccgc ccggcggcct gccggagacg ttcctcgagt cggtggacga gccgctggcg
    13801 cggctggtgc gccggtacgc gcgcacgcac ggcccgttca cgacggcgga gatcggcggc
    13861 cgctacggga tcgacccggc gccggtgctc cgggagctgg agcgcgcggg cgagctggtc
    13921 cgcggcgagc tgcgcccgga gggcaccgag cgcgagtggt gcgacccgga ggtgctgcgg
    13981 aggctgcggc gcgcctcgct ggcgtcgctt cgaaaggagg tggagccggc cgagcaccgc
    14041 gcgctcgcac ggctgctgcc cgagtggcag ggcgtggacg gtgcgccgcc gggcggcgcg
    14101 ggcgtggacc gcctgcgcga ggcgctcgtg ccgctccagg gcatcgcgct cgcgccggag
    14161 gtgtgggagc gcgacgtgct gccgcggcgc gtaggggcct actcgcccac ctggatggac
    14221 tcgctctgcg cggcggg
//
LOCUS       DASVPL010000015        24357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_118600, whole genome shotgun sequence.
ACCESSION   DASVPL010000015 DASVPL010000000
VERSION     DASVPL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24357
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118600"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VF517_00710"
     CDS             <1..306
                     /locus_tag="VF517_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8051484.1"
                     /translation="FASYLAVVEQIGEVSSGGFFLIFWMFQNPPDLLYSLIKGMAMAT
                     VIVIVGTYYGYTASGGPVGVGTATAKSMVLNTVMVHLVGMLGTQIFWGANPRAPIGG"
     gene            309..836
                     /locus_tag="VF517_00715"
     CDS             309..836
                     /locus_tag="VF517_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051485.1"
                     /translation="MLATPDRDAPREAAGLPPVAPLRVGGAGEALARRALRAQIARLE
                     TDLAAQAASARRPATFAELVRGDAKTGDGTAAMARTVPRLLSLGELEAQRDDLAARLA
                     DERADLALLADRQEGFRQLREEMLLDPAAYPDVRVSNADVGEPGCLDWHVRPNFGPLG
                     RLMRWWRVKISSGCP"
     gene            864..2315
                     /locus_tag="VF517_00720"
     CDS             864..2315
                     /locus_tag="VF517_00720"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8051486.1"
                     /translation="MTLVLPAAAAAQGDVVSIPVKFAVENVNRSALPCASDGQSYELA
                     GEIVGPRAVLEGSAPASATLYLHEFSYDDFFHFRAAPGVDYATEMARAGHVSVAIDRL
                     GYDDSPHPPGTATCLGAHADMARQVIDALRAGSYRPAQGRPRGFQRIVLAGHSVGGEV
                     AELEAYSFGGIDALVLFGVVDQGFQPRATQVAAEQGAVCARGGEDSGGAPGYAFYGDT
                     PESVRDLSFYDADPAVVAASERQRHRDPCGDVNSNVPALGSNNAHMGEIEVPVLLLYG
                     LEDGVMTRDAQRQHGEKYTGSADVTTAYFERTAHAMLLERTAPQVRGAVSRWLGERGL
                     ASEASPPAPPKPPTGCPARNAMVVGSDRADRLEGAGGADSLFGLAGDDRIAGKAGGDC
                     IRGGPGRDLLIGDGGADRLHGDAGDDRISGGSSRDRLEGGKGHDRLRGGSGDDRLFAR
                     DGARDLVDCGPGGADRATVDRLDRVRGCERVAR"
     gene            complement(2359..3552)
                     /locus_tag="VF517_00725"
     CDS             complement(2359..3552)
                     /locus_tag="VF517_00725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8051487.1"
                     /translation="MCAASAAALGVLPWAASAPAADTSASGGGRAYVGKPEIKQVNCA
                     SGCERGGKVRNGGSVRLKGSRLSYASKIVFLGGGGKADDVAVAVDPASDRTLKVKVPY
                     NARSGPLSAMASPQVRSKPTRPISIVAPPPPEPTGQLAPASGTADPGAPKLETAVNRG
                     KFFFDGAGGIVFTYRVDAPAAVAVDLVRLTDGAVVQSWSAPAVAANEVQEIRWDGRAG
                     GVAQPDARYAFRAAATGADGAATRSAAEGDAERDAFDLHGNIFPVRATHNFGGAGSQF
                     GAGRAGHSHQGHDVFAKCGARMVAARGGVVKAKKYQSSAGNYVVIDGQDTDVDYFYAH
                     LEQPTPLKVGDRVHTGQPIGTVGQTGNAQGCHLHFELWSGPGWYSGGAPFDPLPHLKA
                     WDSYS"
     regulatory      complement(3588..3767)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            3872..4309
                     /gene="rplM"
                     /locus_tag="VF517_00730"
     CDS             3872..4309
                     /gene="rplM"
                     /locus_tag="VF517_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX8051488.1"
                     /translation="MKTYVATPATRERNWVVIDASGLTLGRLATQIANTLRGKHKPEF
                     TPHCDVGDFVIVVNAERISVTGNKREEKRYYRHSGYPGGLRSRTLAEMLDRRPEEVIR
                     KAVKGMLPRNRLGRAQLRKLKVYAGPEHPHAAQKPEPMEVATR"
     gene            4975..5364
                     /gene="rpsI"
                     /locus_tag="VF517_00735"
                     /pseudo
     CDS             4975..5364
                     /gene="rpsI"
                     /locus_tag="VF517_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            5361..6689
                     /gene="glmM"
                     /locus_tag="VF517_00740"
     CDS             5361..6689
                     /gene="glmM"
                     /locus_tag="VF517_00740"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX8051489.1"
                     /translation="MSGQSTPPKLFGTDGVRGVAGEFLTADLAVALGRAAATLSPTEA
                     PQVLIVRDTRESGPTLEAALAAGVAAAGGHALLGGVLPTPGAALLVRRYGFDLAAVVS
                     ASHNPYRDNGIKFFGPEGTKIPDATEREMERLVQDPPAERADGRVRELHGAGGDYLRE
                     LESRFASLDLSGVRVLLDCANGATYRVAPEIFRRLHADVEAIAADPDGRNINDGVGST
                     HVEALAERVSAGGHDLGFAFDGDGDRVLAVDREGTVVDGDELIAIAALHLRAADRLPG
                     GGVAVTVMTNYGFHTAMREHDIEVATTPVGDRNVLAELIERGWALGGEQSGHIIDTGF
                     VPSGDGVAAALLTLEALAGRDLAARDAMSRLPQKLVNVRVGDRDKLDGATAVWEAVER
                     ESDGLKDRGRVLVRPSGTEPLVRVMVEAPEAAECDAVTARLVEVVERELA"
     gene            6768..8627
                     /gene="glmS"
                     /locus_tag="VF517_00745"
     CDS             6768..8627
                     /gene="glmS"
                     /locus_tag="VF517_00745"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX8051490.1"
                     /translation="MCGIVGYVGDRPCRDLLLRGLEKLEYRGYDSAGVSLLTDGRIES
                     VRAVGNLSYLRDAVARVDDGASVAVAEREPTTGIGHTRWATHGRVTEENAHPHYDTAD
                     RVHIVLNGIVENHVELRRRLTAAGAEFTSETDAEVVAHLIAEHLDGDLVAAVRAAYLE
                     LRGHYSFVAMSADDPDLLVGARKECPLVIGVGEGETFIASAIPAFLKETRRIQLIEDD
                     EVVVVRPQGVEFLSAHTGAPLEREVDEVDWDEAAAEKEGYETFMLKEIHEQPEAVAET
                     IADRVTGETIDLGDIGISDDELRAVRRVVIVACGTSYHAGLVGRYAVEEWGRVPVEMD
                     IASEYRYRNPVVDEHDLVIGITQSGETADTLAAMRLARERGARVIALTNVMGSQATRD
                     ADGVLFTRAGPEVSVAATKTFVAQAACMYMFGLKLAQVRGTLDAGTLRELIGGIRELP
                     HQIQQTIERTDQAAIDAAERFFAESEFCLYLGRHIGLPVCLEGALKLKEISYIPADAY
                     AAGEMKHGPIALLSERTPVICVANDSPVLDKVLSNVAEVRARGAHVIAVATEGSDEVE
                     ATAEAVLWVPRSHWLLQPILAIVPLQLLAYRVARSRGLNVDQPRNLAKTVTVE"
     gene            complement(8634..9272)
                     /locus_tag="VF517_00750"
     CDS             complement(8634..9272)
                     /locus_tag="VF517_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051491.1"
                     /translation="MRIRRPSPAMVVAIVALVMATAGTSIAAVTYATNAGKVDGLDAV
                     RAGSPDKAAGKLIATARSGIRKGTIPNYHLSGVPYSDTYGELFQVPDNQTGGVVTLDE
                     SELGRFSAQCSDENNTAGNENPTGRIVFTSTNAAPVNFARHVGSGGATSGVLQPNTEH
                     AFSIGTANTFRFHAELFGVNVVYEGFVRQVNQGTAEAQCMIVGTVQTMRPEK"
     gene            9361..10881
                     /locus_tag="VF517_00755"
     CDS             9361..10881
                     /locus_tag="VF517_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX8051492.1"
                     /translation="MAPPDWLDPLFSAEEMRAADAYAIGEAGVPSIDLMERASVGLAR
                     VVAAAAAGPGPIRVLVGKGNNGGDGLACARLLREEGRTVDVLAVGDLQETEGDARANL
                     ERLPGAAPETFAPERLDGSASIVDAMLGTGFEGSPREPVAGAIAAINAADAPVIACDV
                     PSGVNASTGEIEGEAVRADTTATFHGPKIGLHVAPGAFHAGRVETVEIGIPRGAPGAA
                     GAGLISDRVLDLVPARTRKGSKFTSGTVVIAGGAAGYTGAPCMVALGAQRSGAGYVQL
                     AVPESAQQAVALRLLEAVTRGLPEEGGMHVPAGVEEVEGMAERAGAVVLGPGLGRSDG
                     AAEFARGVARAIEKPLVIDADGLNAHVGALDSLRERPGPTVLTPHAGELARLMEVESD
                     ELSAHRLRFAREAAERSGCVVLLKGDDTIVAAPGGPVAVSPGGTAALATAGTGDVLAG
                     VLGALLSKGMDVLAAAAAGVRLHVYAGLAAAERLGADHTVAGDVIEGLPEAFRRAR"
     gene            10892..11356
                     /locus_tag="VF517_00760"
     CDS             10892..11356
                     /locus_tag="VF517_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8051493.1"
                     /translation="MAALVREVMSPDPLTASPETGVEALVRLLAEHDLPGVPVVNESG
                     HCVGMVTESDLVITDEEGEDLHIPHYIELMGGLIPLEPLRKFEERLKKAAAATVADMM
                     TSPAVTVEADDPTRKAARLIAESGHNRLPVEDEGRLVGIVTRADVLRALADE"
     gene            11356..12447
                     /gene="alr"
                     /locus_tag="VF517_00765"
     CDS             11356..12447
                     /gene="alr"
                     /locus_tag="VF517_00765"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX8051494.1"
                     /translation="MPAERAVARVDLAAIERNCAHLRGQIAGDAELCAVVKADGYGHG
                     AVASARAALDGGATWLAVATAREAAELRGAELDPPLLVMGALTPADLDLAAMAGAEVV
                     AWTPELADAAVAAGLRVHVKYDSGMGRLGTADPDEARALVERVAASGQLGGLMTHFAT
                     ADERGDDHFPAQLERFGALAEQVRAEHPDAVVHAANSAATLRDPRAHFDLVRCGIAVY
                     GLDPFQEDPAARGLDPALTLTSYVSAIKRFEPGTTAGYGRTWAASEPTWVGVLPIGYG
                     DGWRRGLSNEARVLIGGRSHPLVGTVSMDNVTVDLGPETGAEVGDEAVLIGAQGEERI
                     LAEEVAAALGTINYEVTCGLTQRVRREHG"
     gene            12444..13865
                     /locus_tag="VF517_00770"
     CDS             12444..13865
                     /locus_tag="VF517_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX8051495.1"
                     /translation="MSLDALFEAEAVRAAREALPGRRAWIVGGAVRDARLGRPIVDVD
                     LAVEGEPAAAAQAIARAGRGPAFQLSEEFGAWRALGGGPQRWVCDVSPLQGQTIEEDL
                     LRRDFAANALAVPLEGGDLLDPSGGARDIDERRLRVLPGAYEADRLRPLRLARLATEL
                     GFEPDEATAAATRATAHAVVGASPERVFAELRRILVADAVVEGLERCDRLGLLAAILP
                     ELSELHGVEQSRYHHLDVFGHTIEVLEHQVALERDPAAVFGPELGAALDAQLARVLGD
                     ELNVWQALRLVALLHDVAKPATRGVLPDGRVTFMGHHDVGVEMIDAICARLRTSEKLR
                     TFLGDVTRHHLVLGFLVHERPLARAMVYRYLKHCSPVELEVTVHSCADRMATRGDRSE
                     EAIAVHLELAREMAGEAIAWREHPPRVPVRGDDLARELGIPPGPEIGALLGRLEEAAF
                     TGEAPTREDALALARGLRENPDR"
     gene            13862..14836
                     /gene="corA"
                     /locus_tag="VF517_00775"
     CDS             13862..14836
                     /gene="corA"
                     /locus_tag="VF517_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX8051496.1"
                     /translation="MIVDCAVYADGRRQGDRLELRDAYEAGTASEGSFVWIGLHEPSP
                     DEFDSVAREFDLHELAVEDAIKAHQRPKLEVYEGTVFMVLKTASYIEETETVEIGQIM
                     LFAGTGFVITVRHGQHADIHDVRQRMEQRQDLLRCGTGAVLHAIVDRVVDDYLPVIAG
                     LDDDIEEVESDVFAPGRSNPTERIYKLQREVLQMHRATAPLVEPLRRLAEGHFDFVHE
                     DLRPYFRDVHDHAVRTNEQVEGFRDLLHGVLEANLAQVSVRQNEDMRKISAWVAILAV
                     PTMIAGIYGMNFEHMPELRWEFGYPTVLAVIAVSCSVLYAYFKRVGWL"
     gene            14854..15270
                     /locus_tag="VF517_00780"
     CDS             14854..15270
                     /locus_tag="VF517_00780"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX8051497.1"
                     /translation="MPDSDPDCIFCKILAGELPGEVVQEDEHTVAFMDINPWTRGPAV
                     VIPRRHAKNLYEIEDEDLERTVVAAKRLALQMKERLGCDGINLLNSCEAPAWQTIFHL
                     HVHVIPRYADDPLQLPTRPQRAEPEELARVAADIRG"
     gene            15263..16039
                     /locus_tag="VF517_00785"
     CDS             15263..16039
                     /locus_tag="VF517_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8051498.1"
                     /translation="MADGFRFEQDGPVGVLTLDAPPVNLFSAALVESLERSLDEIEAA
                     GPRALLLRAEGKVFTGGVDVNTFDGLSESDASEFCERLLQITHRVEDLPFPTIACVHG
                     LCLTAGFELSLACDMIWAAESARFGLVEIVVGVSPFMGGTQRVAERAGPARARELVMS
                     GGLYDAAVLERWNVVNRVLPDDELADKTMRFAERLANGPTLANVATKRVVRAFLDGGV
                     RGADAETCGIVAPLFETEDLPRAVRTFLDEGPGKATFEGR"
     gene            16241..16444
                     /locus_tag="VF517_00790"
     CDS             16241..16444
                     /locus_tag="VF517_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8051499.1"
                     /translation="MATGTVKWFNDEKGFGFIKPDEGDRDLFVHHTAINGTGFKSLQE
                     NAKVSYDEEAGDKGPKAVNVTKI"
     gene            16479..16697
                     /gene="infA"
                     /locus_tag="VF517_00795"
     CDS             16479..16697
                     /gene="infA"
                     /locus_tag="VF517_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX8051500.1"
                     /translation="MSKEEKIEMEGEVMEALPNTMFRVKLDNDHEVLGHISGKMRRHY
                     IRILPGDRVKIELSPYDLDRGRITYRHK"
     gene            16694..17578
                     /locus_tag="VF517_00800"
     CDS             16694..17578
                     /locus_tag="VF517_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051501.1"
                     /translation="MIRAARPGARRLAQLVFALGVAGGIALGIIGCVQGVGDADKYGR
                     VTLPGEDVVDLPEGEIALYYEERVTLSDNDSLDAPDGIRVVAVRENERIKSKKRGLSN
                     AISLDGRALDEWGKIEIPRAGKWRIVTRSRESGSNQPAVTLGRGQTDNLVDAGTTAGT
                     VMAAGLGAALLILLLGRIGYTRPTPQQLVAAMPRLPEAYGGSAPAWSPSPGSAPGGAM
                     PGGSAPPTGSPPAWSPPPGIMAAPASPPAPAAASGDPVEVQLRDLERRHAAGEFDEPE
                     YKRRRQAVLDAAFRRPGG"
     gene            complement(17652..18578)
                     /locus_tag="VF517_00805"
     CDS             complement(17652..18578)
                     /locus_tag="VF517_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8051502.1"
                     /translation="MLDVKRMKVLKEVAAEQSFSAAAQKLGYTQSAVSQQVAALERET
                     GTTLIERNPRGIRLTDAGEALVRHAEGILARLADAEAELEAIAGLRGGRLRLGSFPTA
                     GATLMPRAIAKFSRRHPAVELSLVEADPIESLPRLKAGELDLVLVDDTSGVLEQPDLD
                     FEHVLDDPLHLALAVDHPLAKRERVRMKDLADEAWIQPTQACACSVATRKACANAGFE
                     PRVAYESDDFGVVQGLVGAGVGVALIPELALIGERPDIVVRRIDIKPGVRKIYAGILA
                     NGYRSPALDEMLAILKETGSEFAPKPAITAVA"
     gene            18672..18965
                     /locus_tag="VF517_00810"
     CDS             18672..18965
                     /locus_tag="VF517_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051503.1"
                     /translation="MSHRDTILIRSSNEHDAPAIARLAVLDSAPAPSGRMLVAEVDGT
                     MRAALSLDGGRSIADPFAHTAHLVELLRAHAAALDTVRPAPQRAQRHLAPVTV"
     gene            complement(18980..20311)
                     /locus_tag="VF517_00815"
     CDS             complement(18980..20311)
                     /locus_tag="VF517_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8051504.1"
                     /translation="MSHPQDLPLREQAAAIRSGDLDPGELLRATLDRIEERNGALNAI
                     VATFPEESERMLAEAPQGPLHGVPVAIKDEWAIPWRAPRNGSARDALPASESDVFRLL
                     RDSGAVVAGVSQMHFWGAGTTGHISAYGPVGNPWDATRCAGGSSGGSASAVGSRMVAG
                     AVGADGGGSVRLPAAYCGITGLKCTNNVIPRAGHVHRHSALGAAGPMCRDAADARLFG
                     SALLGRELPAGDGAGLRVAVVRDPFWGDLDPAVDEACRDALAASGWQVDEISLEGAAY
                     ALIATVLIITVEALPEFDAEDLAGADPLSIALIKYQRLMPAEAFHRSNRIRSQLRRSL
                     ASAFGNYDLLVWPTIPVGAPPIEAPMVERPSGPSSPEPANIGQTALGNLTGIPGINVP
                     VGRDGNGMPVGLMLQAAWNREEILLDAAEHIERATDREWVDAVPPLPAAAG"
     gene            complement(20308..20499)
                     /locus_tag="VF517_00820"
     CDS             complement(20308..20499)
                     /locus_tag="VF517_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051505.1"
                     /translation="MDAFEQFVRAGMELAGETVNDTDLVIMRIADEVYGSQVRALMAE
                     DLRDVKPELAFDPSRAPTG"
     gene            20551..21225
                     /locus_tag="VF517_00825"
     CDS             20551..21225
                     /locus_tag="VF517_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051506.1"
                     /translation="MKRLMPLAAACLSLALAPSAGADVREIGQPTPFPAASCPENCQA
                     IAQVSGYNAEIGKSKNTFRINRPGRVVAFTMRLGDPNAEQLNYFRTTFGGVSQARVSI
                     LKPAKTKQRHRLLQQSELVNLEPYFGTTPTFALKKSLPVLKGNIIAITVPTWAPAFAH
                     GLENDNAWRSSHHDDDCTNAMPPPAAQMSLGSLRIFGCFYRTARLLYSVHFVLDPTPK
                     TTAPTK"
     gene            21260..21601
                     /locus_tag="VF517_00830"
     CDS             21260..21601
                     /locus_tag="VF517_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051507.1"
                     /translation="MSTEGWIFMVGFRVFDVGLLIVWLVWFFRLRDDDDQADDDGPGG
                     GGPDPSRDDGPGGGGLRLPLGRAKAGPRKREHGGLQRLPRRRGAEPMPNPAPARVRQP
                     TAPVPAHRRAV"
     gene            complement(21611..21880)
                     /locus_tag="VF517_00835"
     CDS             complement(21611..21880)
                     /locus_tag="VF517_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051508.1"
                     /translation="MSERQLYRLRSRSDREVIREVEPGTVYVDRDSGDEFEVVGKILP
                     LAPSPSRLPWAVENLRMCGCSLEQLVPKDVNDCPTCGRRQPAVEG"
     gene            21900..23441
                     /locus_tag="VF517_00840"
     CDS             21900..23441
                     /locus_tag="VF517_00840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8051509.1"
                     /translation="MRILIFHGYLLRGTGSNVYNASLVHALVALGHEVHLLCQDRHAA
                     ELEFVDALGTWEGGTLSVETIREPVRCTAYLPPIGEVLPVYVADEYEGFRALTYPELS
                     DAELDAYLDANVAAVRDVAALAEPDVALANHLIAGPVVLARGLGGSVPYAVKIHGSAL
                     EYVVRPHRERFIDLAREGLAGAGGVLVGSRHTGESLWEVLDDPELPARTRLGPPGVDV
                     HSFHPRTPDEAAARLHALADRLAGGEAAGWGGEAGAPEALRSLDPRRDPIVVYVGKLI
                     VSKGVDLLLAAWPLVTAAVPDARLLVVGFGTYREGLARLVEAFAAGDLDAARELAARG
                     REVEGGPAGELTYLAGFLDGLEGERRDGYLEAAATAFDGLRFTGRLEHGDLPDVLPAC
                     LAQIVPSTFPEAFGMVAVEGAACGALPLSAAHSGLAEVTRALEPAVAAEVAPLLSFER
                     GPGAVEEIASKLVAWLTLPPEDREPARAALSELARERYGWEGVAASVIAAARGELAGL
                     PEPRA"
     gene            23562..24275
                     /locus_tag="VF517_00845"
     CDS             23562..24275
                     /locus_tag="VF517_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8051510.1"
                     /translation="MRPPTNTLKTLAGLLAAGATLLAAGCGGDEADLANGKKLFAGEG
                     QCGSCHALNRAGTKGSVGPDLDKSFAQLVSDGMGRTTIEGTVRNQIAHPRRASAMKPG
                     LVTGEDARDVAAYVAYAAARPGKDSGVLAAAAQPKKGPPIAAKAGTLTIPTDPTGALA
                     YVSDKANAPAGPVTFTSPNESPIQHNIALKPGGPMGPVVGTGGKSTFKVTLKPGNYTF
                     YCSVPGHEAGGMKGTLTVK"
BASE COUNT         3295 a         8931 c         8952 g         3179 t
ORIGIN      
        1 ttcgcgagct acctggcggt ggtcgagcag atcggcgagg tctcatcagg cggcttcttc
       61 ctgatcttct ggatgttcca gaacccgccc gacctcttat acagcctgat caagggcatg
      121 gcgatggcga ccgtcatcgt gatcgtcggc acctactacg gctacaccgc ctcgggcgga
      181 ccggtcggcg tcggcaccgc caccgccaag tcgatggtgc tcaacaccgt gatggtgcac
      241 ctcgtcggca tgctcggcac gcagatcttc tggggagcca acccgcgggc gccgataggg
      301 ggatgagcgt gctcgcgaca cccgaccgcg acgcgccgcg cgaagcggcc ggcctgccgc
      361 ccgtcgcgcc gctgcgcgta ggcggcgccg gcgaggcgct cgcccgccgc gccctgcgcg
      421 cgcagatcgc acggctcgag accgacctcg ccgcacaggc ggcctcggcg cggcgtccgg
      481 ccaccttcgc ggagctggtc cggggggacg cgaagactgg cgatgggacc gcggcgatgg
      541 cacgcaccgt gccgcggctc ctctcgctgg gcgagctcga agcgcagcgc gacgacctcg
      601 ccgcgcgtct cgccgacgag cgcgccgacc tggcgctcct cgccgaccgc caggagggct
      661 ttcgtcagct ccgtgaggag atgctgctcg acccggccgc gtacccggac gtgcgggtct
      721 cgaacgccga cgtcggcgag cccggctgtc tggactggca cgtccggccg aacttcggcc
      781 cgctcggcag gctcatgcgc tggtggcgcg tgaagatctc gtcgggctgc ccgtagatgc
      841 gagtcgcgct cgcggtcctt ctggtgacgc tcgtcctacc cgccgccgcc gcggcgcagg
      901 gcgacgtggt ctcgatcccc gtgaagttcg cggtggagaa cgtgaaccgc tcggcccttc
      961 cctgcgcgag cgacggccag tcgtacgagc tggccggcga gatcgtcggg ccgcgcgcgg
     1021 tgctcgaggg cagcgcgccc gcgagcgcga cgctctacct ccacgagttc agctacgacg
     1081 acttcttcca cttccgcgcg gccccgggcg tcgactacgc gaccgagatg gcgcgggccg
     1141 gccacgtgtc ggtcgcgatc gaccgcctcg gctacgacga cagcccgcac ccgcccggca
     1201 ccgccacctg cctcggcgcg cacgcggaca tggcccgcca ggtcatcgac gcgctccgcg
     1261 ccggcagcta ccgcccggcg cagggcagac cgcgcgggtt ccagcgcata gtcctcgccg
     1321 gtcactcggt gggcggcgag gtggccgagc tcgaggcgta ctcgttcggc gggatcgacg
     1381 cgctcgtgct cttcggcgtc gtggaccagg gcttccagcc gcgcgcgacg caggttgcgg
     1441 ccgagcaggg agcggtatgc gcgcgcgggg gcgaggactc gggcggcgcg cccggctacg
     1501 cgttctacgg cgatacgccc gagagcgtgc gcgacctgag cttctacgac gccgacccgg
     1561 ccgtggtcgc ggcctccgag cgccagcgcc accgcgatcc gtgcggcgac gtgaactcca
     1621 acgtcccggc gctcggatcg aacaacgcgc acatgggtga gatcgaggtg ccggtcctgc
     1681 tgctctacgg gctcgaggac ggcgtgatga cccgcgacgc gcagcgccag cacggcgaga
     1741 agtacaccgg gagcgccgac gtgaccacgg cgtacttcga gcgcacggcc cacgcgatgc
     1801 tgctcgagcg caccgcaccg caggtccgcg gcgcggtgtc gcgctggctc ggcgagcgcg
     1861 ggctcgcctc cgaggcctcc ccgcccgcgc cgccgaagcc gccgaccggg tgtccggcgc
     1921 gcaatgccat ggtcgtggga agcgaccgag ccgaccggct cgagggcgcg ggcggcgcgg
     1981 acagcctgtt cggcctcgcc ggcgacgacc gcatcgcggg caaggccggc ggcgactgca
     2041 tccgcggcgg gcccggacgc gacctgctga tcggcgacgg cggagccgac cggctccatg
     2101 gcgacgcggg cgacgaccgc atctcgggcg gctcgagccg cgaccgactc gagggtggca
     2161 agggacacga ccgcctgcgc ggcggctcgg gcgacgaccg cctgttcgcc cgcgacggcg
     2221 cccgcgacct ggtcgactgc ggcccgggag gcgccgaccg cgcgacggtc gaccggctcg
     2281 accgcgtgcg cggctgcgag cgggtggcgc gctagccgga gcgggtcgcg ccgcctcgcg
     2341 cggcgcgccg gccgcggcct agctgtacga gtcccaggcc ttgaggtgcg gcagcgggtc
     2401 gaacggcgcc ccgccggagt accagcccgg cccggaccag agctcgaagt gcaggtggca
     2461 gccctgggcg ttgccggtct ggccgacggt gccgatcggc tgccccgtgt gcacgcggtc
     2521 gccgaccttc agcggcgtcg gctgctcgag gtgcgcgtag aagtagtcga catcggtgtc
     2581 ctgaccgtcg atgacgacgt agttgccggc cgaggactgg tacttcttgg ccttcaccac
     2641 gccgccgcgc gccgcgacca ttcgcgcgcc gcacttggcg aacacgtcgt ggccctggtg
     2701 cgagtggccc gcccgccccg cgccgaactg cgatccggct ccgccgaagt tgtgcgtggc
     2761 gcggacgggg aagatgttgc cgtggaggtc gaacgcgtcg cgctcggcgt cgccttcggc
     2821 cgcgctgcgg gtggcggcac cgtcggcacc ggtggccgcg gcgcggaatg catagcgcgc
     2881 atcgggctgc gcgacgccgc cggcgcggcc gtcccagcgg atctcctgga cctcgttcgc
     2941 cgcgacggcg ggcgcgctcc agctctggac gacggcgccg tcggtgagcc ggaccaggtc
     3001 gacggccacg gcggctggcg cgtccacccg gtaggtgaag acgatgccgc cggctccgtc
     3061 gaagaagaac ttgccgcggt tgacggcggt ctcgagcttg ggggcgcccg ggtccgcggt
     3121 gccggacgcc ggcgcgagct ggccggtcgg ctcgggcggc gggggagcga cgatcgagat
     3181 cgggcgcgtc ggcttcgacc gcacctgcgg gctcgccatc gccgagagcg ggccgctgcg
     3241 cgcgttgtac ggcaccttca ccttgagcgt gcggtcgctc gcggggtcca cggcgacggc
     3301 cacgtcgtcg gccttgccgc cgccgccgag gaagacgatc ttcgacgcgt acgagagcct
     3361 gctgcccttc agccggaccg agccgccgtt gcggaccttg ccgccgcgct cgcagccgga
     3421 cgcgcagttc acctgcttga tctcgggctt gccgacgtag gcgcgcccgc ccccgctggc
     3481 gctcgtgtcg gcggccgggg cggaggccgc ccaggggagg acgcccagcg cggcggcgct
     3541 cgctgcgcac acgatcgttc ggcggctatg gctcgactgc aaagtccgac ctctctcttc
     3601 agcgcctacg gggttagctg acggactcgc ggcaaaagag cgccgctacg ggtgagcggg
     3661 ccatgctggc cccacccgat tcgcccctgg gagccggtcc atcggctccc aactggttcc
     3721 cccgttcccc cggaccggcg gccccgggga ttcggcaggc aacgacttga agggcggcag
     3781 tataagcgca tcggtcggat ggttcgctct cgggggagcc cttccccggg cctgcaaccc
     3841 agcgcccgcc cgctaccctc cccgagtcct catgaagacc tacgtagcca cccccgcgac
     3901 gcgtgagcgc aactgggtag tgatcgacgc cagcggcctg acgctgggcc gcctcgccac
     3961 ccagatcgcg aacacgctca ggggcaagca caagcccgag ttcacgccgc attgcgacgt
     4021 cggcgacttc gtcatcgtcg tcaacgccga gcggatctcg gtgaccggga acaagcgcga
     4081 ggagaagcgc tactaccggc attccggcta tcccgggggg ttgcgctcgc ggacgctcgc
     4141 tgagatgctc gaccgccggc ccgaggaggt catccgcaag gcggtcaagg ggatgctgcc
     4201 gcgcaaccgg ctcgggcgcg cgcagctacg caagctgaag gtctacgccg ggccggaaca
     4261 cccgcacgca gctcagaagc ccgaaccgat ggaggtcgcc acccgatgat cgacgacgag
     4321 cgcccggacg ctcccgagga gcagcccacg cccggcggcg gcgacgccgc tggcgacgtg
     4381 cccgatccga gcgcttccgc cgatccgagc gagtcgccgg ccgccggcga cgacgccgtc
     4441 gaggacgccg ctcccgccga ggagtcggcc gccgagcccg cttccgagga gcccgccgct
     4501 tctgaggagc ccgccgcaga gcccgctgcc gctgacgcgc cccctgctgc cgaggagcct
     4561 gctgccgagg agtccgccgc agaggagtct cctgctgctg aagagcctcc tgctgctgag
     4621 gagcctgctg ctgctgctgc tgctgctgct gctgcggagc ctgctgcaga ggagcccgcc
     4681 tctgaggagc ccgctgccga gcagcccgcc gcttccgagg accgcccgcg ccgcgacaag
     4741 ggtcgcaagc gcgacgacga ggagaagtcc gagcccgagg tcgtgcccgg cgcccacctc
     4801 gagcccgacc tggtcctcga ggccgaccgg cccgagggcg aggacgacct ctcccgctac
     4861 gcggccgccg acgaggccga cgcccccgcc gacggcgacg cccccgcggg cgagccagcc
     4921 ggcgacggcg acgctgccga gcccgccgcc gcccgctcgg tcctcgacct ctccgccgac
     4981 gctcgctacc gcgccaccgg caagcgcaag accgccgtcg cgcgcgtgat cctgcgcccg
     5041 ggcgacggcg gctacagcat caacggccgc tcgctcgagg acttcttccc gcgcgcggcg
     5101 ctccagaagg ccgcccgcca gccgctcgag gttgccggct tcgagcgccg gatggacgtg
     5161 gtggcccgga tccacggcgg cggcgtctcg gcccaggccg gcgcgctgcg ccacggcgtg
     5221 gcccgcgcgc tcgtcgaggc cgacccggcg ctgcgccccg agctcaagcg gcgcggcctc
     5281 ctcacgcgcg acgcgcgtgc gaaggagcgc aagaaggccg gcctcaagaa ggcccggaag
     5341 cgcccgcagt tctcgaagcg ctgagcgggc agagcacccc cccgaagctc ttcgggaccg
     5401 atggcgtccg cggcgtcgcg ggcgagttcc tcaccgccga cctggcggtc gcgctcggtc
     5461 gcgccgcagc gaccctctcg cccaccgagg ccccgcaggt cctcatcgtg cgcgacaccc
     5521 gcgagtcggg ccccacgctc gaggcggcgc tggccgccgg ggtcgccgcc gcgggcggcc
     5581 acgcgctgct cggcggcgtg ctgccgaccc cgggcgcggc gctgctcgtg cgccgctacg
     5641 gcttcgacct cgccgccgtg gtctccgcct cccacaaccc ctaccgcgac aacggcatca
     5701 agttcttcgg cccggagggc accaagatcc cggacgcgac cgagcgcgag atggagcgcc
     5761 tggtccagga tcccccggcc gagcgtgccg acggccgcgt gcgcgagctg cacggcgcgg
     5821 gcggcgacta cctgcgcgag ctcgagtcgc gcttcgcgag cctcgacctg tccggcgtgc
     5881 gcgtgctgct cgactgcgcc aacggcgcca cctaccgcgt cgctccggag atcttccgcc
     5941 gcctgcacgc cgacgtcgag gcgatcgccg ccgaccccga cggccgcaac atcaacgacg
     6001 gcgtcggctc cacgcacgtc gaggcgctgg ccgagcgcgt gagcgccggc ggccacgacc
     6061 tcggcttcgc gttcgacggc gacggcgacc gcgtgctggc ggtcgaccgc gagggcaccg
     6121 tcgtggacgg cgacgagctg atcgccatcg ccgcgctcca cctgcgggcg gccgaccgcc
     6181 ttccgggcgg cggcgtggcc gtcacggtga tgacgaacta cggcttccac acggccatgc
     6241 gcgagcacga catagaggtg gccaccaccc ccgtgggcga ccgcaacgtt ctcgcggagc
     6301 tgatcgagcg cggctgggcg ctcggcggcg agcagtccgg ccacatcatc gacacgggct
     6361 tcgtgccctc gggcgacgga gtcgccgccg cgctgctcac cctcgaggcc ctagccgggc
     6421 gcgacctagc cgcccgcgac gcgatgtccc gcctgcccca gaagctcgtg aacgtgcggg
     6481 tcggcgaccg cgacaagctc gacggagcca cggcggtctg ggaggcggtc gagcgcgagt
     6541 ccgacggcct gaaggatcgc ggccgcgtcc tcgtccgtcc gtctggcacg gagccgctcg
     6601 tgcgtgtgat ggtggaggcc cccgaggcgg ccgagtgcga cgccgtcacg gcccgcctcg
     6661 tggaggtcgt ggagcgcgag ttggcgtagc cgccgggtgt gggcgaatgc ccaccccgcc
     6721 gataacctcg aaggtcagcc ggcccccgac ggcgaaggaa tcgccccatg tgcggcatcg
     6781 ttggatacgt aggtgacagg ccgtgccggg acctcctgct gcgcggcctc gagaagctcg
     6841 agtaccgcgg ctacgactcc gctggcgtct cgctgctcac cgacggccgg atcgagtcgg
     6901 tgcgcgccgt cggcaacctc tcctacctgc gcgacgccgt cgcccgcgtc gacgatggcg
     6961 cctccgtcgc ggtcgccgag cgcgagccca ccaccggcat cggccacacg cgctgggcca
     7021 cgcacggccg cgtcaccgag gagaacgccc acccgcacta cgacaccgcc gaccgcgtcc
     7081 acatcgtcct gaacgggatc gtggagaacc acgtcgagct gcgccgtcgg ctcaccgcgg
     7141 ccggcgccga gttcacctcg gagaccgacg ccgaggtggt tgcccacctg atcgccgagc
     7201 acctcgacgg cgacctggtc gccgccgtgc gcgccgccta cctcgagctc cgcgggcact
     7261 actcgttcgt cgcgatgtcg gccgacgacc ccgacctgct cgtcggcgcc cgcaaggagt
     7321 gcccgctggt gatcggcgtg ggggagggcg agaccttcat cgcctccgcg atccccgcgt
     7381 tcctgaagga gacgcgccgc atccagctga tcgaggacga cgaggtcgtc gtggtccgcc
     7441 cgcagggggt cgagttcctc tccgcccaca ccggggcgcc gctcgagcgc gaggtcgacg
     7501 aggtcgactg ggacgaggcg gccgccgaga aggagggcta cgagaccttc atgctcaagg
     7561 agattcacga gcagccggag gccgtggccg agacgatcgc cgaccgcgtg accggcgaga
     7621 cgatcgacct gggcgacatc gggatctccg acgacgagct gcgcgcggtg cgccgtgtcg
     7681 tgatcgtcgc ctgcggaacc tcgtaccacg cggggctggt cggtcgctac gccgtcgagg
     7741 agtgggggcg tgtcccggtc gagatggaca tcgcgtccga gtaccgctac cgcaacccgg
     7801 tcgtcgacga gcacgacctc gtcatcggga tcacgcagtc gggcgagacc gccgacaccc
     7861 tcgccgccat gcgcctcgcg cgcgagcgcg gcgcccgcgt gatcgcgctg accaacgtga
     7921 tgggcagcca ggccacgcgc gacgccgacg gcgtgctctt cacccgcgcc gggccggagg
     7981 tgagcgtcgc cgccaccaag accttcgtgg cccaggccgc ctgcatgtac atgttcgggc
     8041 tgaagctcgc ccaggtgcgc ggcacgctcg acgccggcac gctgcgcgag ctgatcggcg
     8101 gcatccgcga gctgccgcac cagatccagc agacgatcga gcggaccgac caggccgcga
     8161 tcgacgccgc cgagcgcttc ttcgccgagt cggagttctg cctctacctg gggcgccaca
     8221 tcgggttgcc ggtctgcctg gagggcgcgc tcaagctcaa ggagatctcg tacatcccgg
     8281 ccgacgccta cgcggccggc gagatgaagc acgggccgat cgcgctcctg agcgagcgga
     8341 cgccggtgat ctgcgtggcg aacgactcgc ccgtgctcga caaggtcctc tcgaacgtcg
     8401 ccgaggtccg cgcgcgcggc gcgcacgtga tcgccgtcgc gaccgagggg agcgacgagg
     8461 tggaggcgac cgccgaggcc gtcctctggg tgccccgctc gcactggctg ctccagccga
     8521 tcctggcgat cgtgccgctg cagctcctcg cgtaccgcgt cgcgaggtcg cgcgggctga
     8581 acgtggatca gccgcgcaac ctcgcgaaga cggtcacggt cgagtaggcc gggctacttc
     8641 tccggccgca tcgtctgcac ggtcccgacg atcatgcact gcgcctcggc ggtcccctgg
     8701 ttgacctgcc gcacgaagcc ctcgtagacc acgttcacgc cgaacagctc ggcgtggaag
     8761 cggaacgtgt tcgcggtccc gatcgagaac gcgtgctccg tgttcggctg gaggacgcca
     8821 ctggtggcgc cgccgctgcc gacgtggcgg gcgaagttga ccggggccgc gttcgtgctc
     8881 gtgaacacga tccttccggt cgggttctcg ttgcccgccg tgttgttctc gtcgctgcac
     8941 tgggcgctga agcggccgag ctcgctctcg tcgagcgtca cgactccacc ggtctggttg
     9001 tccggcacct ggaacagctc gccgtaggtg tcgctgtacg gcactcccga gaggtggtag
     9061 ttcgggatcg tgcccttgcg gatgccgctg cgggcggtgg cgatgagctt gcccgcggcc
     9121 ttgtcggggc tccctgcgcg gacggcgtcg agcccgtcca ccttccccgc gttggtcgcg
     9181 taagtgaccg cggcgatcga cgtccccgcc gtcgccatca cgagcgcgac gatggccacg
     9241 accatcgccg gcgacggtcg cctgattcgc atgctgcctc cctgggtggt tgagcggata
     9301 cccgcgtgaa ccgtgcgggc gggcgaaagt cgcggacccg gcgccggtag gctcgcggcc
     9361 gtggcgccgc cggactggct cgacccgctg ttcagcgcgg aggagatgcg cgccgccgac
     9421 gcgtacgcga tcggcgaggc gggcgtcccg tcgatcgacc tgatggagcg cgcgtcggtg
     9481 ggcctggcgc gcgtggttgc ggccgccgcc gccgggcccg ggccgatccg cgtcctggtc
     9541 ggcaagggca acaacggggg cgacggcctg gcctgcgccc ggctgctgcg cgaggagggg
     9601 cggacggttg acgtgctcgc ggtgggcgac ctccaggaga ccgagggcga cgcgcgcgcg
     9661 aacctcgagc ggctccccgg cgcggcgccg gagacgttcg cgccggagcg gctcgacggc
     9721 tcggcgtcga tcgtcgacgc gatgctcggt accggcttcg agggctcccc gcgcgagccg
     9781 gtggcgggcg ccatcgcggc gatcaacgcc gccgacgcgc cggtgatcgc ctgcgacgtg
     9841 ccctcgggcg tgaacgcctc caccggcgag atcgagggcg aggcggtgcg cgcggacacc
     9901 acggcgacct tccacggccc gaagatcggc ctgcacgtgg cgcccggtgc gttccacgcc
     9961 gggcgagtcg agacggtcga gatcggcatc ccgcgtggcg cccccggcgc ggccggggcc
    10021 ggcctgatct ccgaccgcgt gctcgacctc gtccccgcgc gcacgcgcaa gggctcgaag
    10081 ttcacctccg gcaccgtcgt gatcgccggc ggtgcggccg gctacacggg cgcgccgtgc
    10141 atggtcgcgc tcggagcgca gcgcagcggc gccggctacg tgcagctcgc ggtgcccgag
    10201 tccgcgcagc aggcggtggc gctgcggctg ctcgaggccg tcacgcgcgg gctgccggag
    10261 gagggcggga tgcacgtgcc cgcgggcgtg gaggaggtcg aggggatggc cgagcgggcc
    10321 ggcgcggtgg tgctcgggcc cggcctcggg cgcagcgacg gcgccgccga gttcgcgcgc
    10381 ggcgttgcgc gcgcgatcga gaagccactc gtgatcgacg cggacggcct gaacgcgcac
    10441 gtgggcgcgc tcgactcgct gcgcgagcgg cccgggccga ccgtcctcac cccgcacgcg
    10501 ggcgagctgg cgcggctgat ggaggtcgag tcggacgagc tgtccgcgca ccggctgcgc
    10561 ttcgcgcgcg aggcggcgga gcgcagcggc tgcgtggtgc tgctgaaggg cgacgacacg
    10621 atcgtcgccg cgccgggcgg gccggtcgcg gtgagcccgg gcggcacggc ggccctcgcc
    10681 acggcgggca cgggcgacgt gctcgcgggg gtgctcggcg cgctgctgtc gaagggcatg
    10741 gacgtgctgg cggcggcggc cgcgggggtg cgcctgcacg tctacgcggg cctggcggcc
    10801 gcggagcgcc tgggcgccga ccacaccgtc gcgggcgacg tgatcgaggg tctgcccgag
    10861 gcgttccggc gcgcccggta gcctgaccgt cgtggccgcc ctcgtgcgcg aagtcatgtc
    10921 gcccgacccg ctcaccgcct cgcccgagac gggagtcgag gcgctggtgc gcctgctcgc
    10981 cgagcacgac ctgcccggcg tgccggtggt caacgagtcc gggcactgcg tcgggatggt
    11041 cacggagtcc gacctcgtaa tcaccgacga ggagggcgag gacctgcaca tcccgcacta
    11101 catcgagctg atgggcgggc tgatcccgct cgagccgctg cgcaagttcg aggagcgcct
    11161 caagaaggcg gcggccgcga cggtcgccga catgatgacc tcgcccgccg tgaccgtgga
    11221 ggccgacgac ccgacccgca aggcggccag gctgatcgcc gagagcggcc acaaccgcct
    11281 ccccgtggag gacgagggcc gcctggtcgg gatcgtcacg cgcgcggacg tgctgcgcgc
    11341 gctcgcggac gagtagtgcc cgccgagcgc gcggtcgcac gggtcgacct ggccgcgatc
    11401 gagcgaaact gcgcgcacct gcgcgggcag attgcgggcg acgccgagct gtgcgcggtg
    11461 gtgaaggcgg acggctacgg gcacggcgcc gtggcgagcg cccgggccgc gctggacggc
    11521 ggggccacct ggctcgcggt ggcgaccgcg cgcgaggcgg cggagctgcg cggcgcggag
    11581 ctcgatccgc cgctgctggt gatgggagcg ctcacgcccg ccgacctcga cctggccgcg
    11641 atggcgggcg cggaggtggt ggcgtggacg cccgagctcg cggacgcggc cgtcgccgca
    11701 gggctgcgcg tccacgtgaa gtacgacagc gggatgggga ggctcggcac cgccgacccg
    11761 gacgaggcgc gcgcgctggt cgagcgcgtg gcggcgtcgg gccagctcgg cgggctcatg
    11821 acccacttcg ccaccgccga cgagcgcggc gacgaccact tcccggctca gctcgagcgc
    11881 ttcggtgcgc tcgccgagca ggtccgcgcc gagcacccgg acgccgtcgt gcacgcggcc
    11941 aacagcgccg cgacgctgcg cgacccgcgc gcgcacttcg acctcgtccg ctgcgggatc
    12001 gccgtctacg gactcgaccc gttccaggag gacccggcgg cgcgcgggct cgacccggcg
    12061 ctcacgctca cgagctacgt cagcgcgatc aagcgcttcg agcccggcac caccgcgggc
    12121 tacgggcgga cctgggccgc ctccgagccg acctgggtcg gggtgctccc gatcggctac
    12181 ggcgacggct ggcgccgcgg gctctcgaac gaggcgcgcg tgctgatcgg cggacgctcg
    12241 cacccgctgg tgggcaccgt cagcatggac aacgtgaccg tcgacctcgg gccggagacg
    12301 ggcgcggagg tgggcgacga ggcggtgctg atcggggcgc agggcgagga gcggatcctc
    12361 gccgaggagg tggccgccgc gctcggaacg atcaactacg aggtgacctg cgggctgacg
    12421 cagcgcgtgc gccgggagca cgggtgagcc tcgacgctct gttcgaggcg gaggccgtcc
    12481 gcgcggctcg cgaggcgctg cccggccggc gcgcgtggat cgtcggcggc gcggtgcgcg
    12541 acgcccggct cggccgcccg atcgtggacg tggacctcgc ggtggaaggc gagcccgccg
    12601 ccgccgcgca ggcgatagcg cgcgccgggc gcggcccggc gttccagctc tcggaggagt
    12661 tcggggcgtg gcgcgcgctt ggcggtggcc cgcagcgctg ggtgtgcgac gtctctccgc
    12721 tccagggcca gacgatcgag gaggacctcc tccggcgcga cttcgccgcc aacgcgctgg
    12781 ccgtcccgct ggagggcggc gacctcctcg atcccagcgg cggcgcgcgg gacatcgacg
    12841 agcgccgcct gcgcgtcctc cccggcgcgt acgaggccga tcggctgcgg ccgctgcgcc
    12901 tcgcgcggct cgccaccgag ctgggcttcg agcccgacga ggccaccgcc gccgccacgc
    12961 gcgccaccgc tcacgcggtg gtcggggcct cgcccgagcg cgtgttcgcc gagttgcgcc
    13021 ggatcctggt cgccgacgcg gtggtcgagg gcctcgagcg ctgcgaccgc ctcgggctcc
    13081 tcgccgcgat cctgccggag ctgagcgagc tgcacggggt cgagcagagc cgctaccacc
    13141 acctcgacgt cttcggccac acgatcgagg tgctcgagca ccaggtcgcg ctcgagcgcg
    13201 atcccgcggc ggtgttcgga cccgagctgg gcgcggcgct ggacgcgcag ctcgcccggg
    13261 tcctcggcga cgagctgaac gtgtggcagg cgctgcgcct cgtcgcactg ctgcacgacg
    13321 tggccaagcc ggccacgcgc ggcgtgctcc ccgacgggcg cgtcacgttc atgggccatc
    13381 acgacgtggg cgtcgagatg atcgacgcga tctgcgcgcg cctgcgcacg agcgagaagc
    13441 tccgcacgtt cctcggcgac gtgacccgtc accacctcgt gctcggcttc ctggtgcacg
    13501 agcgcccgct cgcgcgcgcg atggtctacc gctacctgaa gcactgctcc cccgtggagc
    13561 tcgaggtcac ggtgcactcg tgcgcggacc ggatggccac ccgcggcgat cgctccgagg
    13621 aggccatcgc cgtgcacctg gagctggctc gcgagatggc gggcgaggcg atcgcgtggc
    13681 gcgagcaccc gccgcgggtg ccggtgcgcg gggacgacct cgcgcgggag ctcggcatcc
    13741 cgccgggacc cgagatcggg gcgctcctcg gccgtctcga ggaggccgcc ttcaccggcg
    13801 aggcgccgac gcgcgaggac gcactggcgc tcgcgcgggg tctgagggag aatccggacc
    13861 ggtgatcgtc gactgcgctg tctacgcgga cgggaggcgc cagggcgacc gcctggagct
    13921 gcgcgacgcg tacgaggccg gcacggccag cgagggcagc ttcgtctgga tcgggctcca
    13981 cgagccgtcc cccgacgagt tcgactcggt cgcacgcgag ttcgacctcc acgagctcgc
    14041 cgtggaggac gcgatcaagg cgcaccagcg gccgaagctc gaggtctacg agggaaccgt
    14101 cttcatggtg ctgaagacgg cgtcgtacat cgaggagacc gagaccgtcg agatcgggca
    14161 gatcatgctg ttcgccggga ccggcttcgt gatcaccgtg cgccacggcc agcacgcgga
    14221 catccacgac gtccgccaga ggatggagca gcgccaggac ctgctgcgct gcgggaccgg
    14281 cgcggtgctg cacgcgatcg tcgaccgcgt ggtcgacgac tacctgccgg tgatcgccgg
    14341 actggacgac gacatcgagg aggtcgagtc cgacgtgttc gcgccggggc ggagcaaccc
    14401 gaccgagcgg atctacaagc tccagcgcga ggtgctccag atgcaccgcg ccaccgcgcc
    14461 gctggtcgag ccgctgcggc gcctcgccga gggccatttc gacttcgtcc acgaggacct
    14521 gcgcccgtac ttccgcgacg tgcacgacca cgcggtgcgc acgaacgagc aggtcgaggg
    14581 cttccgcgac ctgctgcacg gcgtgctcga ggccaacctc gcccaggtga gcgtccgcca
    14641 gaacgaggac atgcgcaaga tctcggcctg ggtcgcgatc ctcgcggtgc cgaccatgat
    14701 cgccggcatc tacggcatga acttcgagca catgccggag ctgcgctggg agttcggcta
    14761 ccccaccgtg ctggccgtga tcgcggtgtc gtgctcggtg ctctacgcct acttcaagcg
    14821 cgtcggctgg ctctaggggt agtctcccgc gccgtgcccg actccgaccc ggactgcatc
    14881 ttctgcaaga tcctcgcggg cgagctgccc ggcgaggtcg tgcaggagga cgagcacacc
    14941 gtcgcgttca tggacatcaa cccgtggacg cgcggccccg cggtcgtgat cccgcggcgc
    15001 cacgcgaaga acctgtacga gatcgaggac gaggatctcg agcgcaccgt ggtggcggcc
    15061 aagcggctcg cgctacagat gaaggagcgg ctcggctgcg acgggatcaa cctgctgaac
    15121 tcgtgcgagg ccccggcctg gcagacgatc ttccacctgc acgtccacgt gatcccgcgc
    15181 tacgcggacg acccgctcca gctccccacg cgcccgcagc gcgcggagcc cgaggagctc
    15241 gcgcgcgtcg cggccgacat ccgtggctga cgggttccgc ttcgagcagg acggtcccgt
    15301 cggcgtcctg acgctggacg cgccgccggt caacctcttc agcgccgcct tggtcgagag
    15361 cctggagcgc tcgctcgacg agatcgaggc cgccgggccg cgggcgctgc tgctgcgggc
    15421 ggagggcaag gtcttcaccg gcggggtcga cgtgaacacg ttcgacgggc tctccgagtc
    15481 cgacgcgagc gagttctgcg agcggctgtt gcaaatcacg caccgggtcg aggacctccc
    15541 gttcccgacg atcgcctgcg tgcacgggct ctgcctgacg gccgggttcg agctctcgct
    15601 ggcgtgcgac atgatctggg ccgccgagtc ggcgcgcttc ggactcgtcg agatcgtggt
    15661 gggggtcagc ccgttcatgg gcggcaccca gcgcgtggcg gagcgggccg ggccggcccg
    15721 cgcgcgcgag ctcgtgatga gcggcgggct ctacgacgcc gcggtgctgg agcgctggaa
    15781 cgtggtcaac cgcgtcctcc ccgacgacga gctggcggac aagacgatgc gcttcgcgga
    15841 gcggctcgcc aacgggccga cgctcgcgaa cgtggccacc aagcgcgtcg tccgcgcgtt
    15901 cctggacggc ggggtgcggg gcgcggacgc cgagacgtgc ggcatcgtcg ctccgctgtt
    15961 cgagaccgag gatctccccc gggcggtgcg cacgttcctc gacgaaggac ccggcaaggc
    16021 gaccttcgag ggccgctagg accatcccct atccgggtgg tcttggcgcc cccgcgatgg
    16081 ttcagatcgc acggcccatc cttccgccat tccgggtacc cgggctggta acgtgccgcg
    16141 cggcgccgga ccgattccgg cttgggagag ggccagggga ccgcgctcgg agggcgaccg
    16201 acgggagcgg aagcggccag agaggaagga tgcatcgcgc atggcgaccg gcaccgtcaa
    16261 gtggttcaac gacgagaagg gcttcggctt catcaagccg gatgagggcg acagggacct
    16321 cttcgtccat cacacagcga tcaacggaac gggcttcaag tccctccagg agaacgccaa
    16381 ggtctcctat gacgaggagg ccggggacaa gggtccgaag gccgtgaacg tcacgaagat
    16441 ctgagtccgg cgcggccccc gcggctagac tcccgaccgt gtcgaaggaa gagaagatcg
    16501 agatggaggg cgaggtcatg gaggccctgc ccaacacgat gttccgggtg aagctcgaca
    16561 acgatcatga ggttctcggg cacatctccg ggaagatgcg gcgccactac atccgcatcc
    16621 tcccgggcga ccgggtgaag atcgagctct ctccctacga cctcgatcgg ggcaggatca
    16681 cctaccgcca caagtgatcc gggccgcgcg gccgggcgcg cgcaggctcg ctcagctcgt
    16741 gttcgccctg ggcgtcgccg gcgggatcgc gctcggaatc atcggctgtg tgcagggggt
    16801 gggtgacgcc gacaagtacg gccgcgtgac gctgccgggc gaggacgtcg tcgatctgcc
    16861 cgagggcgag atcgcgctct actacgagga gcgcgtcacg ctctcggaca acgacagcct
    16921 ggacgcgccg gacgggatcc gcgtcgtggc ggtccgcgag aacgagcgca tcaagtcgaa
    16981 gaagcggggg ctctcgaacg cgatcagcct cgacgggcgg gcgctcgacg agtggggcaa
    17041 gatcgagatc ccgcgggcgg gcaagtggcg gatcgtcacc cggtcgaggg aatcggggtc
    17101 gaaccagccc gcggtgacgc tcgggagggg ccagaccgac aacctcgtgg acgccggcac
    17161 gaccgccggc accgttatgg cggccggtct gggcgcggcg ctgctgatcc tgctgctggg
    17221 ccggatcggc tacacccgcc cgacgccgca gcagctcgtg gcggcgatgc cgcgcctgcc
    17281 agaggcgtac ggcggctccg cgcccgcctg gtcgccgtcg cccggctccg cgcccggcgg
    17341 ggcgatgccc ggcggctccg cgcccccgac cggctcccca cccgcctggt cgccgccgcc
    17401 cggcatcatg gcggcgcccg cttcaccgcc cgcgccggcg gccgcgagcg gcgatccggt
    17461 cgaggtccag cttcgcgacc tcgagcgccg ccacgcggcc ggcgagttcg acgagcccga
    17521 gtacaagcgg cggcgccagg ccgtgctcga cgccgccttc cgccggcccg gcggctagcc
    17581 actccgcgcc gctaggcgcc tggcgccacc cgcgcggcta gagcccggcg ccaggcgcgc
    17641 cgcgcgcgcc gctaggcgac ggcggtgatc gccggcttcg gcgcgaactc ggacccggtc
    17701 tccttcagga tcgcgagcat ctcgtcgagg gcgggggagc ggtagccgtt ggcgaggatc
    17761 ccggcgtaga tcttccgcac gcccggcttg atgtcgatcc ggcgcaccac gatgtcgggg
    17821 cgctccccga tcagcgcgag ctccgggatc agcgccacgc cgacgcccgc gccgacgagc
    17881 ccctgcacca cgccgaagtc gtcgctctcg tatgccacgc gcggctcgaa gccggcgttc
    17941 gcacacgcct tgcgggtcgc gaccgagcac gcgcacgcct gcgtcggctg gatccacgcc
    18001 tcgtcggcga ggtccttcat gcggacgcgc tcgcgcttgg cgagcgggtg gtcgaccgcc
    18061 agggcgagat ggagcggatc gtcgaggacg tgctcgaagt cgaggtcggg ctgctcgagg
    18121 acgccgctcg tgtcgtcgac caggacgagg tcgagctcgc ccgccttcag ccgcgggagg
    18181 ctctcgatcg ggtcggcctc gaccagcgac agctcgaccg cgggatggcg gcggctgaac
    18241 ttcgcgatcg cgcgcggcat gagcgtcgcg cccgcggtgg ggaaggagcc gaggcgcagc
    18301 cgcccgccgc gcaggccggc gatcgcctcg agctcggcct ccgcgtcggc cagccgcgcg
    18361 aggatgccct cggcgtggcg gaccagcgcc tcacccgcgt cggtcagccg gatgccgcgc
    18421 gggttgcgct caataagggt ggtgccggtc tcgcgctcga gcgcggcgac ctgctgcgag
    18481 acggccgact gcgtgtagcc cagcttctgc gccgccgccg agaacgactg ctccgccgcg
    18541 acctccttca gcaccttcat ccgcttcacg tcgagcataa gggcaactta tcacacggcc
    18601 aagtaacaat cgtttgaatt tataccgccc agcgtcgata gtcctcgtaa cccccacata
    18661 cggagacagc catgagccac cgcgacacca tcctcatccg aagcagcaac gagcacgacg
    18721 cccccgccat cgcgcggctt gccgtactcg acagcgcgcc cgcgccgagc ggccggatgc
    18781 tggtcgccga ggtggacggg acgatgcgcg cggcgctttc gctggacggc ggccgctcga
    18841 tcgcggaccc gttcgcccac acggcgcatc tggtcgagct gctgcgcgca cacgccgccg
    18901 cgctcgacac cgtgcgcccc gcgcctcagc gcgcccagcg ccacctggcg cccgtaacgg
    18961 tctaaaaccg ggaccgccgc tagccggcag cggccggcag cggcggcacc gcgtcgaccc
    19021 actcgcggtc ggtcgcgcgc tcgatgtgct cggcggcgtc caggaggatc tcctcgcggt
    19081 tccacgcggc ttggagcatc aggccgacgg gcatgccgtt gccatcgcgt cccactggca
    19141 cgttgatccc ggggatgccg gtcaggttcc cgagcgcagt ctggccgatg ttcgccggct
    19201 cgggcgacga gggcccggac gggcgctcga ccatcggcgc ctcgatcggc ggcgctccga
    19261 ccggaatcgt cggccatacc aggaggtcgt agttcccgaa ggccgacgcg agggagcgcc
    19321 gtagctgcga gcggatccgg ttcgagcggt gaaacgcctc ggccggcatc aggcgctggt
    19381 acttgatgag cgcgatcgag agcgggtcgg ccccggcgag gtcctcggcg tcgaactcag
    19441 ggagcgcctc gaccgtgatg atcaggaccg tcgcgatcag cgcgtacgcg gctccctcga
    19501 gcgagatctc gtccacctgc cagccggagg ccgcgagcgc gtcgcggcag gcctcgtcga
    19561 cggccggatc gaggtcgccc cagaacgggt cgcgcaccac ggccacgcgc agcccggcgc
    19621 cgtcgccggc gggcagctcg cgcccgagca gggcggagcc gaacaggcgc gcatcggcgg
    19681 cgtcgcggca catcgggccc gccgcgccga gcgcggagtg ccggtgcacg tgccccgcgc
    19741 gcgggatgac gttgttcgtg cacttgagcc cggtgatccc gcagtaggcg gcgggcagcc
    19801 ggaccgagcc gccgccgtcg gcgcccaccg cgccggcgac catgcgcgac ccgacggccg
    19861 acgccgaccc gccggacgat cccccggcgc agcgcgtcgc gtcccacggg ttcccgaccg
    19921 gcccgtaggc cgagatgtga cctgtcgtcc ccgcgcccca gaagtgcatc tgcgagaccc
    19981 cggccaccac ggcgccggaa tcgcgcagca gcctgaacac gtccgactcg ctcgccggga
    20041 gcgcgtcgcg cgcggagccg ttgcgcgggg cgcgccacgg gatggcccac tcgtccttga
    20101 tcgccaccgg gacgccgtgc agcgggccct gcggcgcctc ggccagcatc cgctcggact
    20161 cctcggggaa ggtggccacg atcgcgttga gggcgccgtt gcgctcctcg atccgatcga
    20221 gggtggcgcg cagcagctcg cccgggtcga ggtcgccact gcggatggcg gccgcctgct
    20281 cgcgcagcgg gagatcctgg gggtggctca tccggtgggt gcccgtgacg ggtcgaaggc
    20341 cagctcgggc ttcacgtcgc gcaggtcttc ggccatcaga gcgcgaacct ggctaccgta
    20401 gacctcgtcg gcgatgcgca tgatcacgag gtcggtgtcg tttaccgtct cgccggccag
    20461 ctccataccg gcccggacga actgttcgaa cgcgtccatg tacgtatgca taccgcaacg
    20521 acgaccggag gtgcgtgttc aatgccctcg atgaagcgac taatgccgct cgcggccgca
    20581 tgcctgagcc tcgcgctggc gccctcggcg ggcgccgacg tgcgcgagat cggccagccg
    20641 acgccgttcc cggcggcgag ttgccccgag aactgccagg cgatcgcgca ggtctcgggc
    20701 tacaacgccg agatcggcaa gtcgaagaac acgttccgga tcaaccggcc ggggcgcgtg
    20761 gtcgcgttca cgatgcggct cggcgacccg aacgccgagc agctcaacta cttccgcacc
    20821 acgttcggcg gcgtctcgca ggcgcgcgtg tcgatcctca agccagcgaa gaccaagcag
    20881 cgccaccgcc tgctccagca gagcgagctg gtcaacctcg agccgtactt cggcaccacg
    20941 ccgacgttcg cgctcaagaa gtcgctgccg gtgctgaagg ggaacatcat cgccatcacg
    21001 gtgccgacct gggcgccggc cttcgcccac ggcctcgaga acgacaacgc ctggcgctcg
    21061 tcgcaccacg acgacgactg cacgaacgcg atgcccccgc ccgcggctca gatgagcctg
    21121 ggcagcctcc ggatcttcgg ctgcttctac cgcacggcgc ggctgctcta cagcgtccac
    21181 ttcgtgctcg atccgactcc gaagacaacc gcgccaacca agtagcggcg ggcctccgta
    21241 gcgcgctacc atcgcgcgag tgtccacgga gggttggatc ttcatggtcg gctttcgggt
    21301 tttcgacgtt ggcctgctga tcgtctggct ggtctggttc ttccgcctcc gtgacgacga
    21361 cgaccaggcc gacgacgacg gtcccggcgg cggcggtccg gacccctcgc gcgacgacgg
    21421 tcccggcggc ggcggtctgc gcctcccgct cgggcgcgcc aaggcgggtc cgcgcaagcg
    21481 cgagcacggc gggctccagc ggctgccgcg ccgacgcggc gccgaaccga tgccgaaccc
    21541 cgcgcccgcg cgcgtgcgcc agccgacggc gcccgtgccg gcgcaccgtc gcgcggtctg
    21601 acctgcggag ctagccctct acggcgggct ggcggcgccc gcaggtgggg cagtcgttca
    21661 cgtccttggg cacgagctgc tcgagcgagc agccgcacat gcggaggttc tcgacggccc
    21721 acgggagcct gctcggggac ggcgccaggg gcaggatctt tccgacgacc tcgaactcgt
    21781 caccgctgtc gcggtcgacg tatacggtgc cgggctcgac ctcccggatc acctcacggt
    21841 ccgacctgga ccgaagcctg tagagctggc gctcgctcac ggcggcgaaa gataccgact
    21901 tgcgaatcct catcttccac ggatacctgc tgcgcggcac cggcagcaac gtctacaacg
    21961 cctcgcttgt ccacgccctc gtcgcgctcg ggcacgaggt gcacctgctg tgccaggacc
    22021 gccacgcggc cgagctcgag ttcgtcgatg ctctcgggac gtgggagggg ggcacgctca
    22081 gcgtggagac gatccgcgag ccggtgcgct gcaccgccta cctgccgccg atcggcgagg
    22141 tgctgcccgt ctacgtggcc gacgagtacg agggcttccg cgcgctcacc tacccggagc
    22201 tgtccgacgc ggagctggac gcgtacctcg acgccaacgt ggccgccgtg cgcgacgtgg
    22261 ccgcgctcgc cgagccggac gtcgcgctcg ccaaccacct gatcgccggc ccggtcgtgc
    22321 tggcgcgcgg gctcggcggg tcggtgccct acgcggtgaa gatccacggc agcgcgctcg
    22381 agtacgtggt gcgaccccac cgcgagcggt tcatcgacct cgcgcgcgag gggctcgccg
    22441 gagccggcgg cgtgctggtc gggtcgcgcc acaccggcga gagcctgtgg gaggtgctcg
    22501 acgatccgga gctgcccgcc cgcacgcgcc tcggcccgcc cggcgtggac gtgcactcgt
    22561 tccacccgcg cacgccggac gaggcggccg cgcggctcca cgccctcgcc gaccggctcg
    22621 ccgggggcga ggccgccggg tggggcgggg aggccggcgc gcccgaggcg ctgcgatcgc
    22681 tcgacccgcg gcgcgacccg atcgtcgtct acgtcgggaa gctgatcgtg tcgaagggcg
    22741 tggacctgct gctggccgcc tggccgctcg tcaccgcggc ggtgccggat gcgcgtctgc
    22801 tggtggtggg cttcggcacc taccgcgagg gcctggcgcg gctggtcgag gcgttcgccg
    22861 caggggacct cgacgcggcg cgcgagctgg ccgcgcgcgg gcgcgaggtc gagggcgggc
    22921 ccgccggcga gctcacgtac ttggcgggct tcctggacgg cctcgagggc gagcgtcgcg
    22981 acggctatct ggaggcggcc gccaccgcgt tcgacggact gcgcttcacc gggcggctcg
    23041 agcacggcga cctgccggac gtgctgcccg cgtgcctcgc gcagatcgtg ccgagcacgt
    23101 tccccgaggc gttcgggatg gtggcggtcg agggcgcggc ctgcggggcg ctgccgctgt
    23161 cggcggcgca ctccggactg gcggaggtca cgcgcgcgct cgagccggcg gtggcggcgg
    23221 aggtcgctcc gctgctgtcg ttcgagcgcg gtccgggcgc cgtggaggag atcgcgtcca
    23281 agctcgtggc ctggctgacg ctgccccccg aggaccgcga gccggcccgc gcggcgctgt
    23341 cggagctggc gcgcgaacgc tatggctggg agggcgtggc cgcgagcgtg atcgcggcgg
    23401 cccgcggcga gctggcgggc ctgccggagc cccgcgcctg acgccgcggc cgcggctcca
    23461 tgccgccggt gcccccgtcg ctcccgcggc tccatgccgc cggcgccccc gtcgctcccg
    23521 cggcttcatg ccgccgccgg agcggataat cttcgcccgc cttgcggccc cccaccaaca
    23581 ccctcaagac cctcgccggc ctcctggcgg ccggtgccac gctgctcgcg gccggctgcg
    23641 gcggtgacga ggccgacctc gccaacggca agaagctgtt cgccggcgag ggccagtgcg
    23701 gtagctgcca cgccctgaac cgcgctggca cgaagggcag cgtcgggccc gacctcgaca
    23761 agtcgttcgc gcagctggtc agcgacggca tggggcgcac cacgatcgag gggaccgtcc
    23821 gcaaccagat cgcccacccg cgccgggcca gcgcgatgaa gcccggcctg gtcacgggcg
    23881 aggacgcgcg cgacgtggcc gcctacgtcg cctacgctgc ggcgcgcccc ggcaaggaca
    23941 gcggcgtgct ggcggcggcc gcgcagccga agaagggccc gccgatcgcc gcgaaggccg
    24001 gaacgctcac gatcccgacc gaccccaccg gggccctcgc gtacgtgagc gacaaggcca
    24061 acgcgccggc cggcccggtc accttcacct ccccgaacga gtcgccgatc cagcacaaca
    24121 tcgcgttgaa gcccggcggg ccgatggggc ccgtggtggg caccggaggc aagtcgacct
    24181 tcaaggtcac gctcaagccg ggcaactaca ccttctactg ctcggtgccc ggccacgagg
    24241 ccggcggcat gaagggcacg ctcaccgtca agtaggcggg cggagccggc ggcgcggcgg
    24301 cgctagccag cggcggcgcg tttggctgcg gcggcgcccg ccggctgcgg cggcgcg
//
LOCUS       DASVPL010000016         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_196772, whole genome shotgun sequence.
ACCESSION   DASVPL010000016 DASVPL010000000
VERSION     DASVPL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196772"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF517_00850"
     CDS             complement(<1..243)
                     /locus_tag="VF517_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051511.1"
                     /translation="MTRLLAVPRDWVSSLGDIAKFAARSFGEVFGLRVFRFFGEALRQ
                     AGVLILGSTMVIWSLAFILGLQCGIEGAYFNRSVGAP"
     gene            complement(240..1253)
                     /locus_tag="VF517_00855"
     CDS             complement(240..1253)
                     /locus_tag="VF517_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051512.1"
                     /translation="MRRPVITATATALAMTAAAPAAAAPSLDATFSSTAPGASTSLHP
                     TIDYESTDAAGQQRALRRHVFTFPAGTVYDSLGTEACRASAEELRADGLNACSPRSRV
                     GDGTLRAVATRPPASAAGTLEAGLTLFNASHPKDAPNVEHALLVAITVAGQVQTAFAV
                     PVVGHVATEQIPMTCATPGEQPPCPNGELTARSVDYTIAERAQTIDGRTHRLITTPPS
                     CPPSGRWEFDSAREYADGTTARMGATTPCTPTAAPGTRIALRVSPRSVRRCRAQRFSF
                     TATADGRALPGATVRFADRRAVTGADGRASIVARLCKSGLRRVTAKASGYRKGTATVR
                     VLR"
     gene            complement(1253..2059)
                     /locus_tag="VF517_00860"
     CDS             complement(1253..2059)
                     /locus_tag="VF517_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8051513.1"
                     /translation="MIRAIADRAFLPTKSLMEQVGDMMILTGKTVMSALRPPYPYGGE
                     LVQQFMFALRLCWFPLLISTVALSYGAPGLQAGNFLILFGAIDRLGGFFVLTSIREFG
                     PSVTAIILAGVAGTAITADLGARKIREELDALQVLGVDPVKNLVVPRFLSLMVVTGLF
                     NIYAIIFGILGGMIATMTFKAPLGPFFSTLLTNASTTDLWGSVVKTTCFGAIIAIVCC
                     YKGMTASGGAEGVGRAVNQAVVIAFLGIFAFNYVFTQTLLATHPEISVIK"
     gene            2128..2886
                     /locus_tag="VF517_00865"
     CDS             2128..2886
                     /locus_tag="VF517_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX8051514.1"
                     /translation="MAQSPGSPYYALDRGSELPLGTQLAWRLRSVIASGRLAEGDRLP
                     GVREMAALAGVNVNTVRSVYGRLADEGAIVSEHGRGTFVADRRAAEAELDRVAAAFGG
                     PPRAEGRERVGREARGAAEHAPAGRGATHEARRRATLRAEIAMLERELAAIEVPTNGE
                     TREAPDPRDRPTPQLLTADDLETIRDHLVERLQPLRADRAVARDQRERERHEATRDEI
                     ERPTRPSPTRQRAIATTMPKFETGSGGWSLRWRG"
     gene            2954..>3303
                     /locus_tag="VF517_00870"
     CDS             2954..>3303
                     /locus_tag="VF517_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051515.1"
                     /translation="MEARLKKPKTRAYRSFLYLNGDEVINSLSALEGGDVDEVLIRHS
                     EEGGGEIGAEVGISPAKARGGKKRVKRFEEEVRRKRTYHSAATLLLKKLHEQEALGIV
                     EGDYGSAIYEDLEE"
BASE COUNT          531 a         1111 c         1232 g          429 t
ORIGIN      
        1 gggggcgccg accgagcggt tgaagtaggc gccctcgatc ccgcattgga ggccgaggat
       61 gaaggcgagc gaccagatca ccatcgtcga gccgaggatc aggacgccgg cctggcgcag
      121 cgcctcgccg aagaagcgga acacgcgcag gccgaagacc tcgccgaagc tgcgcgcggc
      181 gaacttggcg atgtcgccga gcgaggagac ccagtcgcgc gggacggcga gcaggcgggt
      241 catcgcagca cccgcacggt cgcggtcccc ttgcggtagc cgctcgcctt ggcggttacg
      301 cggcggaggc cggatttgca cagccgcgcg acgatcgacg cgcggccgtc ggcgccggtg
      361 accgcacgcc ggtcggcgaa gcgcaccgtc gcccccggca gcgcgcgccc gtcggcggtc
      421 gccgtgaacg agaaccgctg cgcgcggcag cggcgcacgc tccgcggcga cacccgcagc
      481 gcgatgcgcg tgccgggcgc cgcggtgggg gtgcacgggg tggtggcgcc catgcgcgcg
      541 gtcgtcccgt cggcgtactc gcgcgcggaa tcgaactccc agcggccgct cggcgggcag
      601 gagggcggtg tcgtgatcag gcggtgcgtg cgcccgtcga tcgtctgcgc ccgctcggcg
      661 atcgtgtagt cgaccgaccg ggcggtcagc tcgccgttcg ggcagggcgg ctgctcgccg
      721 ggcgtcgcgc aggtcatcgg gatctgctcg gtcgcgacgt ggcccacgac cggcaccgcg
      781 aacgcggtct gcacctggcc cgcgaccgtt atcgccacga gcagggcgtg ctccacgttc
      841 ggggcgtcct tgggatggct cgcgttgaag agcgtcagcc cggcctcgag ggtgccggcg
      901 gccgaggcgg gcgggcgcgt ggcgaccgcg cgcagcgttc cgtccccgac ccgcgagcgc
      961 ggcgagcagg cgttgaggcc gtccgcgcgc agctcctcgg ccgacgcccg gcacgcctcc
     1021 gtgccgagcg agtcgtagac ggtgccggcc gggaaggtga acacgtgcct gcgcagcgcg
     1081 cgctgctggc ccgcggcgtc ggtggactcg tagtcgatgg tcggatggag cgacgtggac
     1141 gcgccggggg cggtcgagga gaacgtggcg tcgaggctgg gggccgcggc ggccggcgcg
     1201 gcggcggtca tggcgagcgc ggtcgcggtc gccgtgatga ccgggcgccg catcacttga
     1261 tcaccgagat ctcggggtgg gtcgcgagca gcgtctgcgt gaagacgtag ttgaacgcga
     1321 agatccccag gaacgcgatc accaccgcct ggttcacggc gcggcccacg ccctccgccc
     1381 cgcccgaggc ggtcatcccc ttgtaacagc agacgatcgc gatgatcgcg ccgaagcacg
     1441 tggtcttcac gaccgatccc cacaggtcgg tggtggacgc gttcgtgagc agcgtcgaga
     1501 agaacggccc gagcggggcc ttgaaggtca tcgtggcgat catgccgccg aggatcccga
     1561 agatgatcgc gtagatgttg aacaggccgg tgacgaccat caggctcagg aagcgcggta
     1621 ccacgaggtt cttgacgggg tcgacgccga gcacctggag cgcgtcgagc tcctcgcgga
     1681 tcttgcgcgc gccgaggtcg gcggtgatcg ccgtgccggc cacccccgcg aggatgatcg
     1741 cggtcacgct cgggccgaac tcgcggatcg acgtgagcac gaagaagccg ccgagccggt
     1801 cgatcgcgcc gaacaggatc aggaagttcc ccgcctggag acccggggcg ccgtagctca
     1861 gggcgacggt cgagatcagc agcgggaacc agcacaggcg cagcgcgaac atgaactgct
     1921 ggaccagctc gcccccgtac ggatagggcg ggcgcagcgc cgacatgacc gtcttgccgg
     1981 tcaggatcat catgtccccg acctgctcca tcaggctctt ggtggggagg aacgctctgt
     2041 cggcgatggc gcggatcacg actgatccgg tgttctagca cactttctcg cagtgtgtgt
     2101 ccggttatgc cattaaagtg tggtctgatg gcacagagcc ctggcagccc ctactacgcg
     2161 ctcgaccgcg gcagcgagct cccgctcggg acgcagctcg cctggcggct gcgctccgtg
     2221 atcgcgtcgg ggcggctcgc cgagggcgac cgcctgcccg gggtgcgcga gatggctgcg
     2281 ctcgcagggg tcaacgtgaa cacggtccgg tcggtctacg gccggctggc ggacgagggc
     2341 gcgatcgtct ccgagcacgg ccgcggcact ttcgtggcgg accgccgcgc ggcggaggcg
     2401 gagctggatc gcgtggcggc cgcgttcggt gggccgccgc gagccgaagg ccgcgagcgc
     2461 gtgggccgcg aagcgcgcgg cgcggccgag cacgcgccgg ccgggcgtgg cgcgactcac
     2521 gaggctcgcc gccgggccac cctccgcgcg gagatcgcga tgctcgagcg cgagctggcc
     2581 gcgatcgagg ttccgaccaa cggcgagacg cgcgaagcgc ccgatccgcg cgaccggccc
     2641 accccccagc tcctcacggc cgatgacctc gagacaatcc gcgaccatct ggtcgagcgc
     2701 ctccagccgc tgcgcgccga ccgcgccgtg gcgcgcgacc agcgcgagcg cgagcgccac
     2761 gaggcgacgc gcgacgagat cgagcgaccg actcgcccat cgccgacccg ccagcgcgcg
     2821 atcgcgacga ccatgccgaa gttcgagacc ggaagcggcg gctggtcgct caggtggcgc
     2881 ggctagcccg aacagagcaa gaggcacaca tagcgtctcg aggcagccgc gcagccgcat
     2941 acactgcgac ccagtggaag cgcgtctcaa gaaaccgaaa acccgcgcct atcgcagctt
     3001 tctatacctc aacggtgacg aggtcatcaa ctcgctgtcg gccctcgaag gaggcgatgt
     3061 cgatgaggta ctgattcggc attccgagga ggggggcggc gaaatcggcg ccgaggtcgg
     3121 gatttcacca gctaaggcgc gaggcgggaa gaagcgcgta aagcgctttg aagaggaggt
     3181 gcgccgaaag cggacctatc actcggctgc cacgctacta ctgaaaaagc tccatgaaca
     3241 agaggccctc gggatcgtcg agggagacta tggctccgcc atttacgaag atcttgaaga
     3301 gca
//
LOCUS       DASVPL010000017        12507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_236437, whole genome shotgun sequence.
ACCESSION   DASVPL010000017 DASVPL010000000
VERSION     DASVPL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236437"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VF517_00875"
     CDS             complement(<1..967)
                     /locus_tag="VF517_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8051516.1"
                     /translation="MSGRRVLITGLSTFWGGKLAQALERDDRFEAIIGVDDKDPTREL
                     ERTEFVRVSNQHALLRRIVEAAEIDTVVDSRLVVDSTQTSSRKAHENNVIGTMNILAA
                     CSHSTVRSFVFKSSAHFYGTAQDDPAFFTESMGRPHPPRTPIEKDIVEAEASVRDYAE
                     KNPHVKTTIMRMSNVLGPGVETAHTRFFGLPAVPTIFGFDPRYQFVHEDDVVAAAAHA
                     VQNELSGIYNVAADGVLALTEVASLLGKPPAPILMPFGTGLTASALRRIGVRIPPEML
                     GQLRFGRGVDNRKLKASGFRYRYTSREAVLAFGEHMRLAPVLRGTG"
     gene            complement(978..1886)
                     /locus_tag="VF517_00880"
     CDS             complement(978..1886)
                     /locus_tag="VF517_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX8051517.1"
                     /translation="MPNRSSLRVPDPEVADLAPSDPPGELTRREDDLRAHLPGVEPDR
                     ELNDWGRSERIEGLLDRTILDFLYNNWFRVETEGIENIPATGGALLVSNHSGALPPDA
                     TMIAKSIREEHPHPRNLNITVEHFFKGYPGFSMLMPKIGCVAAHPANVQRLLYDEQQL
                     VLVFPEGRKGTEKLFKDRYRLRRFGRGGFVEAAMRARVPIVPVCVIGAEEAAPIFAHL
                     NPLARLTGLTYFPLTPTFPHFGLVGMAGYLPAKFRIKFLPPIHFDEEGLDRDKSLVQS
                     VAQDVRARIQENVWDMVGKRQSVWFG"
     gene            complement(1900..2361)
                     /locus_tag="VF517_00885"
     CDS             complement(1900..2361)
                     /locus_tag="VF517_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051518.1"
                     /translation="MARPRALAAVALVALAAALVALAAVSAPTPVLAASQVRAPRSGS
                     EYTSGAPRDVLLRVSGRSVEIVAITFPCGKTFARASLSDFPLKRTSRGYRFNADANAI
                     VTYANGKPDENGAVHISGRFSLDARTLRGHIAAKTRRCGSTGDLSWKAKKV"
     gene            complement(2361..3188)
                     /locus_tag="VF517_00890"
     CDS             complement(2361..3188)
                     /locus_tag="VF517_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051519.1"
                     /translation="MTRGASVAAAFALALVAAAPAAAAPYTTRSGPERPNPVFGVTYE
                     GSGSYATTFHGEPPNDGGDDDTNDAHDSSRQSWDLGFRHGLALPDCAKAATDGSSACS
                     AVTGLTGARGATVMTGRVDHRHVDGLYSQLDRTVRCRLRGATSPRSKVDAELSVRHLP
                     DSAAFAVTARSPLTTVITAFQSQCPKQGDSIDRILDFYAMPGFSFDGAYGPDRWFTST
                     DVVIPESVFRGSTVIKVPLRLTKAGRSPKGCAVEHPSYERCRTGGSWRGVLTFTRRG"
     gene            complement(3251..3808)
                     /locus_tag="VF517_00895"
     CDS             complement(3251..3808)
                     /locus_tag="VF517_00895"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051520.1"
                     /translation="MARVALDIDSTLHHYWDLLDAIARERFGVALPYDEQHEWGITKL
                     DRGDLIECIRETHSEENIVNAEPYPGAVEVVARWHEQGHWIHVTSHRAVATREATERW
                     LERIAMPYDDLHCSYDKVTRCVELGIDVLVDDSPVNLEAAREAGIAAATIVHPWNARL
                     VEEGAAIGGRDWPELATRLAPLLAG"
     gene            3831..4835
                     /locus_tag="VF517_00900"
     CDS             3831..4835
                     /locus_tag="VF517_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8051521.1"
                     /translation="MAKKRFPRPVPPAAPWHAGRAGDDYGVRDEPDWRTIDWSEHLRR
                     VEIGGRAVNYVDIGPRESDEPATPVVFVHGISGNWQNWLENIPRFAQHRRVVALDLPG
                     SGESEDPAGKISMSGLGATVDALAEHLDLGEVALVGNSMGGFISAETTIQFPERVERL
                     VLVSAAGITTTDLARTPVMAWGRAIAMSGTRSAAEVRMAILRPRLRHLVFSLIMRHPS
                     RIPADTLFEISKGAGPDAFLDTLKAILEYDFRERLPEIRCPTLIVWGANDAIIPSRDA
                     YEYERLIPGTQPVVMLEDTGHVAMIERPVTFNDTVLEFLDGPTPDQGQGVGEPEAAPA
                     "
     gene            complement(4915..6588)
                     /gene="ilvD"
                     /locus_tag="VF517_00905"
     CDS             complement(4915..6588)
                     /gene="ilvD"
                     /locus_tag="VF517_00905"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX8051522.1"
                     /translation="MTETRRLRSRTILEGPDRAPARAYLKGIGYDDDALRRPIVGIAN
                     TWTETMPCNFHLRRLAERVKDGVRAAGGTPMEFNTIAISDGITMGTEGMRTSLVSREL
                     IADSIELVARGHMFDAVVALSGCDKTIPGCVMALARLDVPSVMLYGGSIAPGSFRGKD
                     VTIQDVFEAVGSHAAGDMSDADLAELESVASPGAGACGGQYTANTMATAFEMLGISAM
                     GTSMVPAEEGKKGQVAEDVGRLVMQLLADDVRPSTLITPDSLENAIACVAATGGSTNA
                     VLHLMALAKDAGVDLALDDFDRVSERTPLLADLKPSGRFVATDLWRAGGVRLVAKRLQ
                     DAGLLHADAPTVTGRTIGEEAADADEQQGQEVIRALDDPLKPTGGLAILRGNIAPDGC
                     VVKVSGSTRTAHTGPARVFESEEDAFAAVKAQEIKDGDVVVIRNEGPAGGPGMREMLG
                     VTAALMGEGLGETVALLTDGRFSGATRGLMAGHVAPEAARGGPIAAVRDGDTISFDVE
                     TRRLDVELSEDEIAERVKAYESPPPSADRGVLGKYARHVGSAAEGALTT"
     gene            complement(6585..7271)
                     /locus_tag="VF517_00910"
     CDS             complement(6585..7271)
                     /locus_tag="VF517_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051523.1"
                     /translation="MLLALLVLVLLAAVLVAPLALRAAAARRGRARSRARDRAAARAE
                     RRALRDPGRERRAEQRARELLRSCVNDEEWAMYRDLGFIRVHGTLRSEGPGRALQRRR
                     RRLEYSQHPDQPAPPQQQQAPGYAYLIYPHKPIVAFVPETGRLLSEYCVKFPDLTAAV
                     GATRLPDSDDVLAKWMALTGDERELIGRANMHLPGRQIDPGRVRRDLWRLAEWERQRR
                     GSDSVEARRR"
     gene            complement(7280..7513)
                     /locus_tag="VF517_00915"
     CDS             complement(7280..7513)
                     /locus_tag="VF517_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051524.1"
                     /translation="MKPGHGEVLLTEGDPRLREDEERLVEEFRRQLDEGMWAAVPTTR
                     TGSGRREALMVRSYGDIPQDADRVIFFPRAAGG"
     gene            complement(7629..8519)
                     /gene="thiD"
                     /locus_tag="VF517_00920"
     CDS             complement(7629..8519)
                     /gene="thiD"
                     /locus_tag="VF517_00920"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX8051525.1"
                     /translation="MSALLSELMAVPRLLSIAGSDSGGGAGIQADLKAFACAGAHGMT
                     AITAITAQNTVGVSAVHQVPPDMIVAQVAAVAEDIGVDAVKIGMLGTSETIAAVAEAL
                     DTLPGGTPVVLDPVMVAESGAVLLDEDARDALREMLVPRATVVTPNIPEARVLTESPG
                     GDTSPRELAVAVHAYGPRAVVVTGGHSEEGTDFLYDGIALTEIPGERHPGGAAHGSGC
                     THSSTLAACLARDGDLHAAAREARRVASEAVRLGLRGIGAGPGPVDVFGLELDQRAAV
                     EPWVDRESPIEEAPEREIPF"
     gene            complement(8516..9655)
                     /gene="dprA"
                     /locus_tag="VF517_00925"
     CDS             complement(8516..9655)
                     /gene="dprA"
                     /locus_tag="VF517_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX8051526.1"
                     /translation="MTACAPCLRRSALIGFLAAPIASLLESRRRAPQILSLPDEELID
                     AVGKAWRERALAARKSFDAEAARLEIEDTGLFAICRHDPGYPAPLAALGDGPAVLYGA
                     GSQSRLAELVAEPMVAVVGARRASAYGLEVARDLARGLSAARVTVVSGMALGIDAAAH
                     RGALDAGGNTIAVLACGAERAYPRTNSALYGRIAETGVVLGELPPRTPPRRWTFPARN
                     RIMAGLAAVTVVVEAAERSGSLITARFAEDLGRDVAAVPGRVTSRMAAGSNRLLRDGA
                     HVIRDAADVLDALFGAGGGDGRTAPPRPPRPPRPPRLSPPAEPALEPRLRRVLDAIEG
                     GASVDAAAPVAGLTAAEARAALGRLELLGLLRRDALGGYERAVVR"
     gene            complement(9652..11169)
                     /locus_tag="VF517_00930"
     CDS             complement(9652..11169)
                     /locus_tag="VF517_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX8051527.1"
                     /translation="MLASVATFSIEGIESREVTVEVDLRRGLPVFTIVGLPDRAVREA
                     RERVRAALLNSGLEFPLQRLTANLAPANLRKAGPSFDLALAVAVLAASEQLPMDEVAR
                     LAVSGELSLSGALRPAFGAVAVAAGARRAGYERLVVPVENAPEAALVDGIEVLGVESL
                     ERLVALVRGDWHPEPAVPVRGAGLPPANAPDLADVRGQADARRALEIAAAGGHNLLMV
                     GPPGVGKTMLARRLPGILPPPTHEEAVEITQVLSVAGLGDGRLASRRPFRAPHHTISA
                     SGLVGGGNLPRPGEITLAHRGVLFLDELAEFSRSALEALRQPLEHGSVEVMRGQRTLQ
                     FPAAAMLVGACNGCPCARDRGECTCNEVDRARYHRRLSGPLLDRIDIVCPLTRAPLAP
                     VKAGDPRPEPSSRVRERVVAARERQLARLDGTGAVCNADMDARLTGQHVRVGPGVHDR
                     LHTAACAGALTARGHDRVLRLARTIADLVGADRVEAQHVDEALSYRLGARAAVAA"
     gene            complement(11169..11558)
                     /locus_tag="VF517_00935"
     CDS             complement(11169..11558)
                     /locus_tag="VF517_00935"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX8051528.1"
                     /translation="MSPDPRRTRGSLGEQIAADHLVDSGYEILDRNFRTRRGEIDLIV
                     ADADAIVFCEVKTRVAGGRSGPALALDAVGPAKRRRLRRLAVAWLYERRASGRATHRE
                     RLRFDAIGITITPGGRLLALEHIPDAF"
     gene            complement(11781..>12507)
                     /locus_tag="VF517_00940"
     CDS             complement(11781..>12507)
                     /locus_tag="VF517_00940"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX8051529.1"
                     /translation="SAEAAAAIAEDAPAPVGAATLRPRPSKAAGPRGRKLFAFDRQFE
                     RRFVAGADEAGRGSLAGPLVAAAVLFDLDRLTLGDRRALARLNDSKQHSEAGREELYP
                     LVLRAAARSTIVVRCPRGIDERGLHKTNLEALRTALRTVACPDAICLVDGFRVKDLGY
                     EQRAVVGGDSCSAAIASASVLAKVTRDRYMRRAHERHPDWDFATNVGYSTPEHRAAIR
                     EFGISPLHRLSFASIAYQQMTLG"
BASE COUNT         1630 a         4357 c         4793 g         1727 t
ORIGIN      
        1 cgccggtgcc gcgcagcacc ggcgccagcc gcatgtgctc accgaacgcg aggaccgcct
       61 cgcgcgaggt gtagcggtag cggaagccgc tcgccttgag cttgcggttg tccacgccgc
      121 ggccgaagcg gagctggccg agcatctcgg gcgggatcct cacgccgatc cggcgcaggg
      181 cggacgccgt gaggcccgtg ccgaacggca tcaggatcgg ggcgggcggc ttgccgagca
      241 gcgaggcgac ctcggtgagc gccagcaccc cgtcggcggc gacgttgtag atgccggaca
      301 gctcgttctg caccgcatgc gcggcggccg cgaccacgtc gtcctcgtgc acgaactggt
      361 agcgcgggtc gaagccgaag atcgtcggga ccgccggcag cccgaagaag cgcgtgtggg
      421 cggtctccac acccgggccg agcacgttcg acatgcgcat gatcgtggtc ttgacgtgag
      481 ggttcttctc ggcgtagtcg cgcacgctcg cctcggcctc gacgatgtcc ttctcgatcg
      541 gcgtccgcgg cgggtgcggg cgtcccatcg actcggtgaa gaacgccggg tcgtcctgag
      601 ccgtgccgta gaagtgggcc gacgacttga acacgaagct gcgcacggtc gagtggctgc
      661 acgcggcgag gatgttcatc gtcccgatga cgttgttctc gtgggccttg cgcgagctgg
      721 tctgcgtcga gtcgaccacg agccggctgt cgaccaccgt gtcgatctcc gccgcctcga
      781 cgatccgccg cagcagcgcg tgctggttgc tgactcgcac gaactcggtg cgctcgagct
      841 cacgcgtcgg gtccttgtcg tcgacgccga tgatcgcctc gaagcggtcg tcgcgctcga
      901 gggcctgggc cagcttgccg ccccagaagg tcgacagccc ggtgatgagt actcgcctgc
      961 cgctcaccgg tgccccctta cccgaaccag accgactgtc gcttgcccac catgtcccac
     1021 acgttctcct ggatgcgggc gcgcacgtcc tgcgcgacgc tctggacgag cgatttgtcg
     1081 cggtcgaggc cctcctcgtc gaagtggatc ggcgggagga acttgatgcg gaacttcgcc
     1141 ggcaggtagc ccgccatccc gaccagcccg aagtgcggga acgtgggcgt gagcgggaag
     1201 taggtgaggc cggtgagccg cgcgagcgga ttcaggtggg cgaagatcgg cgcggcctcc
     1261 tcggcgccga tcacgcagac cgggacgatc ggcacacgtg cgcgcatggc ggcctccacg
     1321 aagccgccgc ggccgaagcg acgcagccgg tagcggtcct tgaagagctt ctcggtgccc
     1381 ttgcggccct cggggaagac gagcacgagc tgctgctcgt cgtagagcag gcgctgcacg
     1441 ttcgcggggt gggccgccac gcagccgatc ttcggcatca gcatgctgaa gccgggatag
     1501 cccttgaaga agtgctcgac ggtgatgttg aggttgcgcg ggtgcggatg ctcctcgcgg
     1561 atcgacttgg cgatcatcgt ggcgtcgggc gggagcgcgc cggagtggtt cgagacgagc
     1621 agcgcgccgc cggtggcggg gatgttctcg atgccctcgg tctcgacgcg gaaccagttg
     1681 ttgtagagga agtcgaggat cgtgcggtcg agcaggccct cgatgcgctc ggagcggccc
     1741 cagtcgttca gctcgcggtc gggctcgacc cccggcaggt gggcccgcag gtcgtcctcg
     1801 cggcgtgtga gctcaccggg cggatccgac ggcgcgagat cggccacctc cgggtccggc
     1861 acgcgcaggc ttgagcggtt cggcatccgc gcgatggtgt cagaccttct ttgctttcca
     1921 gctcaggtcg cccgtgctgc cacagcgcct cgtcttggcg gctatgtggc cgcggagcgt
     1981 gcgggcgtcg agtgagaacc ggccgcttat gtgcacggcg ccgttctcgt cgggcttccc
     2041 gttggcgtag gtgacgatcg cgttggcgtc cgcattgaag cggtagcccc tgctggtgcg
     2101 cttcagcggg aagtcgctga gcgaagcacg ggcgaacgtc ttgccgcacg gaaaggtgat
     2161 cgcgacgatc tccaccgacc ggcccgacac ccggagcagc acgtcgcgag gggccccgct
     2221 ggtgtactcg ctcccggacc tgggcgcgcg cacctgggag gcagcgagca cgggtgtggg
     2281 tgcggagacg gcggcgagcg cgaccagggc ggcggcgaga gccacgagtg cgaccgcggc
     2341 gagcgcgcgc gggcgggcca tcagccgcgc ctcgtgaagg tcaacacgcc ccgccaggag
     2401 ccgccggtcc ggcagcgctc gtacgagggg tgctcgacgg cgcagccctt gggagacctg
     2461 cccgccttgg tgaggcggag gggcaccttg ataacggtcg agccgcggaa gacggactcg
     2521 gggatgacga cgtcggtcga ggtgaaccag cgatcgggcc cgtaggcgcc gtcgaagctg
     2581 aacccgggca tcgcgtagaa gtcgaggatg cggtcgatgc tgtcgccctg cttgggacac
     2641 tgggactgga acgcggtgat caccgtcgtg agcgggctgc gggcggtgac cgcgaacgcg
     2701 gccgaatcgg gcaggtggcg caccgacagc tcggcgtcca ccttgctccg cggggaggtc
     2761 gcgccgcgca ggcggcacct caccgtgcgg tcgagctggc tgtagagccc gtccacgtgc
     2821 cggtgatcga ccctgccggt catcacggtg gcccccctgg cccccgtcag cccggtcaca
     2881 gcggagcagg cgctcgaccc gtcggtcgcc gccttcgcac agtcggggag ggcgaggcca
     2941 tgccggaacc cgaggtccca ggactggcgg ctcgagtcgt gcgcgtcgtt cgtgtcgtcg
     3001 tcgccaccgt cgttcggggg ctcgccgtgg aacgtcgtcg cgtacgagcc cgacccctcg
     3061 taggtgacgc cgaagaccgg gttcgggcgc tccggccccg accgcgtggt gtagggggcg
     3121 gcggccgcgg gcgcggctgc cacgagtgcc agtgcgaatg cggctgcgac cgatgctccg
     3181 cgcgtcatgg ggcccagcgt agccgcagaa agccggtccc gcacgtcgtc gcgggcgggc
     3241 ggtcgctggc tcagcctgcc agcagtgggg cgagcctggt tgccagctcc ggccagtccc
     3301 ggccaccgat ggcggcgccc tcctcgacga gccttgcgtt ccagggatgc acgatcgtgg
     3361 cggcggcgat gcccgcctcc ctcgcggcct cgaggttcac cgggctgtcg tccacgagca
     3421 cgtcgatccc cagctcaacg cagcgggtga ccttgtcgta ggagcagtgc aggtcgtcgt
     3481 agggcatggc gatgcgctcg agccagcgct cggtggcctc gcgcgtggcc acggcgcggt
     3541 ggctcgtcac gtggatccag tggccctgct cgtgccagcg ggccacgacc tccaccgccc
     3601 cggggtaggg ctccgcgttg acgatgttct cctcgctgtg ggtctcgcgt atgcactcga
     3661 tcaggtcgcc gcggtcgagc ttcgtgatcc cccactcgtg ctgctcgtca tagggcagcg
     3721 cgacgccgaa gcgctcgcgc gcgatcgcgt cgagcaggtc ccagtagtgg tgcagcgtcg
     3781 agtcgatgtc gagggctacc cgggccacga ggtgggtacc gtagaggccg atggcgaaga
     3841 aacggttccc ccgccccgtg cctcccgcgg ccccctggca tgccggtcgc gcgggcgacg
     3901 actacggcgt tcgcgacgag ccggactggc gcacgatcga ctggagcgag cacctgcggc
     3961 gggtggagat cggcggccgc gcggtcaact acgtcgacat cggtccgcgt gagtccgacg
     4021 agccggccac gccggtcgtg ttcgtccacg ggatctccgg caactggcag aactggctcg
     4081 agaacatccc gcgcttcgcg cagcaccggc gcgtggtggc gctcgacctg ccggggtccg
     4141 gcgagtccga ggaccccgcc gggaagatct cgatgtcggg gctcggcgcg acggtcgacg
     4201 ccctagcgga gcacctggac ctcggtgagg tggcgctggt cggcaactcg atgggcggct
     4261 tcatctcggc cgagaccacg atccagttcc ccgagcgcgt ggagcggctc gtgctcgtgt
     4321 cggcggcggg gatcacgacc accgacctcg cgcgcacgcc cgttatggcg tggggccggg
     4381 cgatcgcgat gagcggcacg cgcagcgccg ccgaggtgcg gatggcgatt ttgcgcccgc
     4441 gcctgcgtca cctggtcttc tccctgatca tgcgccaccc gagccggatc cccgccgaca
     4501 cgctgttcga gatctcgaag ggcgcggggc ctgacgcgtt cctcgacacg ctgaaggcca
     4561 tcctcgagta cgacttccgc gagcggctgc cggagatccg ctgcccgacg ctgatcgtct
     4621 ggggcgcgaa cgacgcgatc atcccctcgc gcgacgccta cgagtacgag cgcctgatcc
     4681 ccggcacgca gcccgtcgtg atgctggagg acaccgggca cgtggcgatg atcgagcggc
     4741 cggtgacctt caacgacacc gtgctcgagt tcctcgacgg gccgacgccg gaccagggcc
     4801 agggcgtcgg cgagccggag gccgcgccgg cgtagtcgcc gggcgggcgc ggcgggcgcg
     4861 ggcctcgcga tgcgcccggc gggcgcccgc ggccgtggcc gtgcggtggc gggcctaggt
     4921 ggtgagcgcg ccctcggcgg ccgagccgac gtggcgcgcg tacttgccga gcaccccgcg
     4981 gtcggcgctc ggcggcggcg actcgtaggc cttcacgcgc tcggcgatct cgtcttcgct
     5041 cagctccacg tcgagccggc gtgtctccac gtcgaagctg atcgtgtcgc cgtcgcggac
     5101 ggccgcgatc ggccccccgc gggcggcctc gggggcgacg tgaccggcca tcagcccgcg
     5161 cgtggctccc gagaagcggc cgtcggtgag gagggcgacg gtctcgccga gcccctcccc
     5221 catcagcgcc gccgttacgc cgagcatctc gcgcatgccg gggccgccgg cggggccctc
     5281 gttgcgaatc accaccacgt cgccgtcctt gatctcctgc gccttcaccg cggcgaacgc
     5341 gtcctcctcg gactcgaaca cgcgtgcggg accggtgtgg gccgtgcggg tggagccgga
     5401 gaccttcacc acgcagccgt cgggcgcgat gttgccgcgc aggatggcga ggccgccggt
     5461 gggcttgagc ggatcgtcga gcgcccggat gacctcctgg ccctgctgct cgtccgcgtc
     5521 cgcggcctcc tcgccgatgg tgcggccggt cacggtgggc gcgtcggcgt ggaggaggcc
     5581 cgcgtcctgg aggcgcttgg ccacgaggcg cacgccgccc gcgcgccaga ggtcggtggc
     5641 gacgaagcgg ccgctgggct tgaggtcggc cagcagcggg gtgcgctcgc tgacgcggtc
     5701 gaagtcgtcg agtgccaggt ccacgcccgc gtccttggcg agggccatca ggtgcagcac
     5761 ggcgttggtc gacccgccgg tcgcggccac gcaggcgatc gcgttctcga gcgagtccgg
     5821 ggtgatgagc gtgctcggcc tgacgtcgtc ggccagcagc tgcatcacga gccggcccac
     5881 gtcctcggcg acctggccct tcttgccctc ctcggccggg accatggagg tgcccatcgc
     5941 cgagatgccg agcatctcga acgcggtggc catcgtgttg gcggtgtact gaccgccgca
     6001 ggcgcccgcg cccggcgacg ccactgactc gagctcggcg aggtccgcgt cgctcatgtc
     6061 gcccgcggcg tggctgccga ccgcctcgaa cacgtcctgg atcgtcacgt ccttgcccct
     6121 gaagctgccg ggggcgatcg agccgccgta gagcatcacg ctcggcacgt cgaggcgagc
     6181 cagggccatc acgcagccgg ggatcgtctt gtcgcagccc gacagggcga ccaccgcgtc
     6241 gaacatgtgg ccgcgcgcga cgagctcgat cgagtcggcg atcagctcgc ggctcacgag
     6301 cgaggtcctc atgccctcgg tgcccatcgt gatcccgtcg ctgatcgcga tcgtgttgaa
     6361 ctccatcggc gtgccacccg cggcgcgcac gccgtccttg acgcgctcgg cgagacggcg
     6421 caggtggaag ttgcacggca tcgtctcggt ccaggtgttg gcgatgccga cgatcggccg
     6481 ccgcagggcg tcgtcgtcgt agccgatgcc cttgaggtag gcgcgcgcgg gggcgcggtc
     6541 cggaccctcg agaatggtcc ggctgcggag tcggcgtgtc tccgtcaccg gcggcgagcc
     6601 tcgacggagt cgctgccgcg gcgctggcgc tcccactcgg cgagccgcca gaggtcacgg
     6661 cgtacccggc ccgggtcgat ctggcggccg gggagatgca tgttcgcgcg cccgatcagc
     6721 tcgcgctcgt cgccggtgag ggccatccac ttggcgagca cgtcgtcgga gtcaggcagc
     6781 cgagtggcgc ccaccgccgc ggtgaggtcg gggaacttga cgcagtactc gctcagcagg
     6841 cgtccggtct cggggacgaa cgcgacgatc ggcttgtgcg ggtagatgag gtaggcgtag
     6901 ccgggggcct gctgctgctg gggcggggcc ggctggtccg ggtgctggga gtactcgagc
     6961 cggcggcggc ggcgctggag cgcgcggccg gggccctcgc tgcggagggt gccgtggacg
     7021 cggatgaagc cgaggtcccg gtacatcgcc cactcctcgt cgttcacgca cgagcgcagg
     7081 agctcgcggg cgcgctgctc ggcgcggcgc tcgcggcccg gatcgcgaag cgcccggcgc
     7141 tcggcgcggg cggcagcgcg gtcgcgcgca cgactgcggg cccggccgcg gcgggccgct
     7201 gcggcgcgca gcgcgagtgg agcgacgaga accgccgcga gcagcacgag caccagcagg
     7261 gcgaggagca tcggccggct cagcctccgg ccgcgcgcgg gaagaagatc acgcggtcgg
     7321 cgtcctgcgg gatgtcgcca tagctgcgga ccatgagcgc ctcgcgccgg ccactgcccg
     7381 tccgcgtcgt gggcaccgcg gcccacatcc cctcgtcgag ctggcggcgg aactcctcga
     7441 cgagccgctc ctcgtcctcg cgcagccgcg gatcgccctc ggtcaggagg acttcgccat
     7501 ggccgggctt catgcggagg aacttgatcg ggaggactcc tgtggcggcg ttgctgtgag
     7561 tggcggcgcg gcgctggaca tctcgcctcg atggcgttgc ccgcctccgc gccggcgcag
     7621 cgattatgtc aaaacgggat ctcgcgctcc ggggcctcct cgatcggact ctcgcggtcc
     7681 acccacggct cgacggcggc gcgctggtcg agctcgaggc cgaacacgtc gaccggtccg
     7741 ggaccggcgc cgatcccgcg caggccgagg cggaccgcct ccgaggcgac gcgcctggcc
     7801 tcgcgggccg ccgcgtggag gtcgccgtcg cgggcgaggc acgccgccag cgtggacgag
     7861 tgggtgcagc cggacccgtg ggcggcgccc cccggatggc gctcgccggg gatctccgtg
     7921 agcgcgatgc cgtcgtagag gaagtcggtg ccctcctcgg agtgcccgcc ggtcacgacc
     7981 accgcgcgcg gaccgtaggc gtgcacggcg acggcgagct cgcgcgggct cgtgtcgccg
     8041 ccggggctct cggtgagaac gcgggcctcc gggatgttcg gcgtcacgac cgtggcgcgt
     8101 gggacgagca tttcgcgcag tgcgtcccgc gcgtcctcgt cgaggagcac cgcaccgctc
     8161 tcggccacca tgaccggatc gaggacgacg ggtgtgccgc cggggagcgt gtcgagcgcc
     8221 tcggcgacgg cggcgatcgt ctcgctcgtg ccgagcatcc cgatcttcac ggcatcgacg
     8281 ccgatgtcct cggccacggc cgccacctgg gccacgatca tgtccggcgg gacctggtgc
     8341 accgcgctca ccccgaccgt gttctgggcg gtgatcgccg tgatcgccgt catcccgtgg
     8401 gcgccggcgc acgcgaacgc cttcaggtct gcctggatgc cggccccgcc gcccgagtcg
     8461 gagccggcga tggagagcaa tcgcgggacc gccatgagct ccgagagtag ggcgctcatc
     8521 tgaccaccgc ccgctcgtag ccgccgagcg cgtcgcgtcg caggaggccg aggagctcga
     8581 gccgcccgag cgcggcgcgc gcctccgccg ccgtgagccc ggccaccggc gccgcggcgt
     8641 ccacggaggc gccgccctcg atggcgtcga gcacgcggcg gagccggggc tcgagcgccg
     8701 gctcggcagg tgggctcagc cgcggcggcc gcggcgggcg cggcgggcgc ggcggagcag
     8761 tcctgccgtc cccgccgccg gcgccgaaga gggcgtcgag cacgtcggcg gcgtcgcgga
     8821 tcacgtgcgc gccgtcgcgg agaaggcgat tcgagccggc cgccatgcgc gaggtgacgc
     8881 ggcccggcac cgcggcgaca tcgcggccca ggtcctcggc gaagcgggcg gtgatgagcg
     8941 agccggagcg ctcggcggcc tccaccacga ccgtgacggc ggcgaggccg gccatgatgc
     9001 ggttgcgcgc cgggaaggtc cagcgccgcg gcggcgttcg cgggggaagc tcgccgagta
     9061 cgaccccggt ctcggcgatc cgcccgtaga gcgcgctgtt cgtgcgtggg taggcgcgct
     9121 cggcgccaca ggcgagcacg gcgatggtgt tgccgccggc gtcgagggcg ccgcggtggg
     9181 cggccgcgtc gatcccgagc gccatgccgc tgacaacggt gacccgagcc gcgctcaggc
     9241 cacgcgcgag gtcgcgggca acctccaggc cataggcgga cgcacgccgc gcgcccacga
     9301 ccgcgaccat cggctcggcc acgagctccg cgagccgcga ctgcgatccg gccccgtaca
     9361 gcacggccgg cccgtccccc agcgcggcca gcggcgccgg gtagccgggg tcgtgacggc
     9421 agatcgcgaa caggccggtg tcctcgatct cgagacgggc cgcctcggcg tcgaagctct
     9481 tgcgggcggc gagcgcgcgc tcgcgccacg ccttcccgac cgcgtcgatc agctcctcgt
     9541 ccggcagcga caggatctgc ggtgcgcggc gacgggactc gagcaggctc gcgatcggcg
     9601 ccgcgaggaa gccgatcagc gcactgcggc gaaggcacgg ggcgcacgcc gtcatgccgc
     9661 caccgccgcc cgggctccga gccggtagga cagggcctcg tcgacgtgct gggcctcgac
     9721 gcggtcggca ccgacgaggt cggcaatcgt gcgggcgaga cgcagcacgc ggtcgtggcc
     9781 gcgagccgtg agggcgcccg cacaggccgc ggtgtggagc ctgtcgtgga cgcccgggcc
     9841 cacgcggacg tgctggccgg tgaggcgggc gtccatgtcc gcgttgcaca ccgccccggt
     9901 cccgtcgagc cgcgcgagct gccgctcacg ggccgcgacc actcgctcgc gcacccgcga
     9961 ggagggctcc ggccggggat cccccgcctt cactggagcg agcggcgcgc gcgtgagcgg
    10021 gcagacgatg tcgatgcggt cgagaagcgg tccgctcagg cggcggtggt agcgggcccg
    10081 atccacctcg ttgcacgtgc actcgccccg gtcgcgtgcg cacgggcagc cgttgcaggc
    10141 tccgaccagc atggccgcgg ccgggaactg gagggtgcgc tgcccgcgca tgacctcgac
    10201 ggatccgtgc tcgagcggtt ggcgcagcgc ctcgagtgcc gagcgggaga actccgcgag
    10261 ctcgtcgagg aacaggacgc cgcggtgtgc gagggtgatc tccccgggcc gcggcagatt
    10321 gccgccgccg acgaggccgg aggcggagat cgtgtggtgg ggagcgcgga acggccgccg
    10381 cgaggcgagc ctgccgtcgc cgaggccggc cacgctgagc acctgcgtga tctcgacggc
    10441 ctcctcgtgg gtggggggcg ggaggattcc cggcaagcgc cgagccagca tcgtctttcc
    10501 cacgccgggc gggccgacca tcagcaggtt gtggccgccg gcggcggcga tctcgagggc
    10561 acggcgggcg tcggcctgac cgcgcacgtc ggcgaggtcc ggtgcgttcg cgggcggaag
    10621 gccggcgcct cgtacgggga ccgcgggttc ggggtgccag tctcccctga ctagcgcgac
    10681 gagccgttcg aggctctcca cgccgagcac ctcgattccg tccacgagcg cggcctccgg
    10741 cgcgttctcg accgggacca cgaggcgctc gtagccggcc cggcgggcgc ccgcggccac
    10801 ggccacggcg ccgaaggccg gacgcagggc gccgctcagc gagagctcgc cggagacggc
    10861 gaggcgtgcc acctcgtcca tcggaagctg ctcgctcgcc gccagcacgg cgaccgccag
    10921 ggcgaggtcg aagctcggcc ccgccttgcg taggttcgcc ggggcgaggt tcgcggtgag
    10981 gcgctggagc gggaactcga ggccggagtt gagcagggcc gcccgcacgc gctcgcgcgc
    11041 ctcgcggacc gcgcggtccg ggagcccgac gatcgtgaag accggcagcc cacggcgcag
    11101 gtccacctcg acggtgacct cccggctctc gatgccctcg atcgagaagg tggcgacgct
    11161 cgccagcatc agaacgcgtc cgggatgtgc tcgagggcga ggaggcggcc gcccggggtg
    11221 atcgtgatgc cgatcgcgtc gaagcggagg cgctcgcgat gcgtggcgcg accgctcgct
    11281 cggcgttcgt acagccacgc gacggcgagg cggcgcaggc ggcggcgctt cgcggggccg
    11341 acggcgtcga gcgcgagcgc cgggcccgag cgacctccgg ccacgcgcgt cttgacctcg
    11401 caaaagacga tcgcgtcggc atcggccacg atcaggtcga tctcgccgcg acgggtccgg
    11461 aagttgcgat cgaggatctc gtagccggag tcgacgaggt ggtcggcggc gatctgctcg
    11521 ccgagtgagc cgcgggttcg gcgggggtcg ggggacatgc cgcgaccgta cgcctgcgcg
    11581 cgttacaggt gtgtttcgta tgtgacgatt tgcgcgccgg tgtgttgcgg gagagtgaaa
    11641 agagcgtgcg cacagggaac tggtcgatct aatgcccgca tagcgggtac aaagtcgacc
    11701 agctgctgcg ggtgggcctg gccgctgtcg gcgagcgcgg cgctacgagc gggcgtggcg
    11761 ctgccggcgg cgcggggccg ctagcccagc gtcatctgct ggtacgcgat cgacgcgaac
    11821 gacagccggt gcaacggcga tatcccgaac tcgcggatcg cggcgcggtg ctcgggggtc
    11881 gagtagccga cgttcgtggc gaagtcccag tccgggtggc gctcgtgcgc gcggcgcatg
    11941 tagcggtcgc gggtgacctt cgctagcacc gaggccgacg cgatcgcggc gctgcacgag
    12001 tcgccgccga ccacggcgcg ctgctcgtag ccgaggtcct tcacgcggaa gccgtcgacc
    12061 aggcagatcg cgtccgggca cgcgacggtg cggagcgccg tcctgagggc ctcgaggttc
    12121 gtcttgtgga ggccgcgctc gtcgatgccg cgcgggcagc ggaccacgat cgtcgagcgt
    12181 gcggccgcgc gcaggaccag cgggtagagc tcctcgcggc cggcctcgct gtgctgcttc
    12241 gagtcgttga gccgcgcgag ggcgcggcgg tcgccgagcg tgaggcggtc gaggtcgaac
    12301 aggaccgccg ctgcgaccag ggggcccgcg agggagccgc ggccggcctc gtcggcgccg
    12361 gccacgaagc ggcgttcgaa ctggcggtcg aaggcgaaca gcttgcgccc gcgcggtccg
    12421 gcggccttgg agggccgcgg gcggagcgtg gcggcgccga cgggcgcggg cgcgtcctcg
    12481 gcgatcgcgg cggcggcctc cgcggac
//
LOCUS       DASVPL010000018         8156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_353377, whole genome shotgun sequence.
ACCESSION   DASVPL010000018 DASVPL010000000
VERSION     DASVPL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353377"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VF517_00945"
     CDS             complement(<1..511)
                     /locus_tag="VF517_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051530.1"
                     /translation="MSLTSAEHRGLRELYASTRQLANHWGVLADRLGGVHAEALRDGA
                     AAAERLLEELEPRMEEHGLAGYPAAKSAGGSLSGMRNLADAALERNQALRVAVLDVQH
                     LGTLLPYLAELARTRGDEGLARWYGGWRRRLAAVEKRAREQAVAAGSDPDAAVQPVLE
                     GAAGRAAHGL"
     gene            558..1229
                     /locus_tag="VF517_00950"
     CDS             558..1229
                     /locus_tag="VF517_00950"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8051531.1"
                     /translation="MDPASGTNLTALEELASDPGSRKRFLRMMGGGAAAATLSAVIAA
                     CGDERTTGNQFGGAGVGTAQYGQGDAGIVAYALFLERIETAFYEGAVASGKLSGRALE
                     LFQRFGQDEAAHAKTLEGALHQLGGKEARVPKFRFVFENQAGIVQTAGQLETVGAGAY
                     LGQVRRIQSKQVLSAALSIHSVEGRHAAALAVLRGQAITPDGAFASPSPSSDVLRQVT
                     PFLST"
     gene            1226..1849
                     /locus_tag="VF517_00955"
     CDS             1226..1849
                     /locus_tag="VF517_00955"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8051532.1"
                     /translation="MSGIGDIRVGGMTRGAFIARSVIAAGALSGAAAAGPALTRALAH
                     GEQGAFTGGDVGIVNFALTLEKIEAEFYKRALAVQGIEPNVKKVLESIAKNETEHVQS
                     LTQALAGLGGKPNPAPQVKLPALSDQAQVLRVAVELEDTGVAAYNGAATQLQSSDLLQ
                     AVASIAQVEARHAGALRELAGQFPTVAPFDRVLSGEQADEAFHRLTG"
     gene            1882..2862
                     /locus_tag="VF517_00960"
     CDS             1882..2862
                     /locus_tag="VF517_00960"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX8051533.1"
                     /translation="MAELSDQLTKYLTDAYSIEQQALAQLRTAPDLVGDPELARIFSE
                     HLTETEGQEERIGALLEERGASPSRIKDMVMAAGGKAFVLFARSQPDTTGKLTAHAHS
                     YEALEEASYEMLLGVAERAGEDEVVDAARSIRDQETRMRERLAGAFDAAAEQSLREVD
                     PDDLDQQLTKYLADAHAIEAQAIQLLQRGPKIGGVPELEQLYERHLEQSRDQQRRVEE
                     RLDALGGSPNRLQDAAMRLGALNWGAFFQAQPDTPGKLAAFAYAFEHLEIGGYELLKR
                     VAARAGDQETVAMADSIIVEERAMAGSVQALFAKALDASLDAQGVGGGAG"
     gene            2875..3309
                     /locus_tag="VF517_00965"
     CDS             2875..3309
                     /locus_tag="VF517_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8051534.1"
                     /translation="MDTSKIGKQYPPFEYEVGREKIREYANAVGETNPVHHDRAAAQA
                     AGYRDVVAPPMFVVVYSAGAMGPAILDPEVGINLMLMVHGGQEFIWGEPVCAGDTVTT
                     TGSVKDMYEKDGRKFYVFESVSTNQDGQETARATWTNIVRGG"
     gene            3311..3700
                     /locus_tag="VF517_00970"
     CDS             3311..3700
                     /locus_tag="VF517_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX8051535.1"
                     /translation="MDAGDQIPELRVTPDKYLPHRYAGASGDFNPIHIDPEFATNVGL
                     PGTILHGLYSMSQVARAQTAAAGGEPRSLKRLAVQFRGMGFPEQEIVVTGTVREVRDG
                     RAVIDTEAAQGDNRIIRNAEAEIEVSE"
     gene            3730..4752
                     /gene="hrcA"
                     /locus_tag="VF517_00975"
     CDS             3730..4752
                     /gene="hrcA"
                     /locus_tag="VF517_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX8051536.1"
                     /translation="MTARQSELLRRLVEAHVSLGQPVGSKWLAENCDMPWGPSTIRAE
                     LARLEELGLLQHPHTSAGRVPTDHGYRQYADALLAGGTTAELAAPVVRLPAVRAEVDE
                     AMRATTEQLSQVTNLLAIVSAPALGTTTIKHVEVLLLQPQVAMVVVITSTGGVTKRVA
                     SYRRPVDPGIVNWAHSYLNEVLGGMAVGARMVHSKLRDPELSDAEREFLATLEPAFSE
                     LDESAPDDLYVGGAARLMSEDRLQELSQINDLMKVLERRVVLLSMLRSALEEPRVYLR
                     IGTENETPELRSWTVVAANYGIARRNLGTVSVVGPTRMDYPTAIASVRQAAAELSRFV
                     GDLYDE"
     gene            4749..5870
                     /gene="dnaJ"
                     /locus_tag="VF517_00980"
     CDS             4749..5870
                     /gene="dnaJ"
                     /locus_tag="VF517_00980"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8051537.1"
                     /translation="MKRDYYEVLGVSRGAEEREIKKAFRRLARELHPDVNKHDPDAEE
                     KFKEAAEAYEVLTDSERRSVYDRYGHDGLQSSGFSSNFSGFGSFGDIFDAFFGGDSPF
                     GGGMRSGASQGRDVAVAVEITLEDAARGASVEVGYDAVEMCEHCRGNGAEPGTPIETC
                     PRCEGAGTLRAVQRTAFGQLVRQAVCDTCGGDGKLAKEPCRECRGRGRQASRKTISVD
                     VPAGIADEQRIRLTGRGHAGERGGPAGDLYVLVRVREDERFVRDGNDLVSQVSISAPE
                     AALGTTVAVDTLDGPEEIEVEAGTQPGTVVTLRGRGMPSLRHGQRGDQRVLVNVLIPQ
                     RLSAQQREMLERFRETLTDENLRGAEDDGFFARVRRSFR"
     gene            5878..6681
                     /locus_tag="VF517_00985"
     CDS             5878..6681
                     /locus_tag="VF517_00985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX8051538.1"
                     /translation="MIRLALRASVEDAERVLAALIELAPSGVEQVDGPGWVEYAVYGM
                     PGELPSLPEGPAEVGGARVTVRGEPVPDDWAERWKRFHVPVLVGGRLWVRPPWEQEAV
                     RPGVHEIVIDPGQAFGTGTHPTTRLCLELMLELDPRGSFADLGCGSGVLSIAAAKLGF
                     RPVCAYDSDRAAVAATLSNARDNGVALDRIERFDLRGAAPPVADVVAANLMRPLLLQV
                     RQSLRELPRVLVLSGLLEAEADEVVAAYAPLVERRRLVDRGWAAVLLAH"
     gene            complement(6720..>8156)
                     /locus_tag="VF517_00990"
     CDS             complement(6720..>8156)
                     /locus_tag="VF517_00990"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX8051539.1"
                     /translation="GLLLGAIVSATDGAAIFGLLRNSTLERKLARTLEGESGLNDPVA
                     ILLVLGFIEWIEEPGYGLLDMLGLFAQQLGIGVVIGVAIGSGAVYAFRRAQLETAGLY
                     PVASLATAGLAFGAAEFIHGSGFLAVYLSGLILGTAGIPAKRTIITFHQGMAWVAQIA
                     MFLALGLLVFPSELGDVWFEGTVLALVLVLVARPVGTVIGTAIDRFTSRERLVLGWAG
                     LRGAVPVVLATFPVIEDVPGSGEFFNIVFFLVVISTLLQGTTFEPLAKRLGLTTNQPA
                     LPSPLAEAGTIRRLGAEVLEYPVGAEDAIVGARVRDLGLPREAVVNVIVREDQAIPPR
                     GSTRLRAADRVHVLMRHEAAAQVPALTRRWRDGPIGPRPRAPRSPQGREPIMTARPWR
                     DADGDPAQPGAIGGRRVVDQLRVRRDRPGGLFVLEDGRYALAGPVLAVGGRTQVSAWA
                     RRRMSQVSPDERAWLQTVIGALATEVPE"
BASE COUNT         1190 a         2901 c         3034 g         1031 t
ORIGIN      
        1 gaaggccgtg cgccgcgcgt ccggcggcac cctcgagcac cggctggacg gcggcgtcgg
       61 ggtccgaccc cgccgccacg gcctgctcgc gggcgcgctt ctcgaccgcc gcgagccggc
      121 ggcgccagcc gccgtaccag cgcgccaggc cctcgtcccc gcgggtgcgg gccagctcgg
      181 cgaggtaggg gagaagcgtg ccgaggtgct gcacgtcgag cacggccacg cgtagcgcct
      241 ggttgcgctc gagcgcggcg tcggccaggt tgcgcatccc ggagagcgag ccgcccgcgc
      301 tcttcgccgc cgggtagccc gccaggccgt gctcctccat ccgcggctcg agctcctcga
      361 gcagccgctc ggccgcggcc gcgccgtcgc gcagcgcctc cgcgtggacg ccgccgaggc
      421 ggtcggcgag cacgccccag tggttggcga gctggcgcgt ggacgcgtac agctcgcgca
      481 gcccgcggtg ctcggcggag gtgaggctca tggtggggaa gctaccgccg agcgggtcgc
      541 gataccgtcc ccgcccgatg gatccggcca gcggcacgaa tctgaccgcc ctcgaggagc
      601 tcgcctccga cccgggctcg cgcaagcgct tcctgcggat gatgggcggc ggggcggccg
      661 cggccacgct ctcggcggtc atcgcggcct gcggcgacga gcgcacgacc ggcaaccagt
      721 tcggcggcgc cggggtcggg accgcgcagt acgggcaggg cgacgcgggg atcgtcgcct
      781 acgcgctgtt cctcgagcgg atcgagaccg ccttctacga gggcgcggtc gccagcggca
      841 agctgagcgg gcgcgcgctc gagctgttcc agcgcttcgg gcaggacgag gccgcgcacg
      901 cgaagacgct cgagggcgcg ttgcaccagc tcggcggcaa ggaggcccgc gtcccgaagt
      961 tccggttcgt cttcgagaac caggccggga tcgtgcagac cgcgggccag ctcgagaccg
     1021 tcggcgccgg ggcctacctt ggccaggtcc gccgcatcca gagcaagcag gtgctctcgg
     1081 cggcgctgtc gatccactcc gtcgagggcc gccacgccgc tgcgctcgcg gtgctgcgcg
     1141 gccaggcgat cacccccgac ggcgcgttcg ccagcccgtc cccgagctcc gacgtcctgc
     1201 ggcaggtcac gccgttcctg agcacgtgag cggcatcggc gacatccggg tcggcggcat
     1261 gacgcgcggc gcgttcatcg cgcgcagcgt gatcgccgcg ggtgcgctgt ccggcgccgc
     1321 cgcggccggc cccgcgctca cgcgggcgct tgcccacggc gagcagggtg cgttcacggg
     1381 cggcgacgtc ggaatcgtga acttcgcgct gacgctcgag aagatcgagg ccgagttcta
     1441 caagcgtgcg ctcgcggtcc agggcatcga gccgaacgtg aagaaggtgc tcgagtcgat
     1501 cgccaagaac gagaccgagc acgtgcagtc gctcacccag gcgctcgcgg gactcggcgg
     1561 caagcccaat ccggcgccgc aggtgaagct cccagcgctg tcggaccagg cgcaggtgct
     1621 gcgggtggcg gtcgagctcg aggacacggg cgtggccgcg tacaacggcg ccgcgacgca
     1681 gctccagtcg tccgacctgc tccaggccgt cgcgtcgatc gcgcaggtcg aggcgcggca
     1741 cgcgggcgcg ctgcgcgagc tggccgggca gttcccgacg gtcgctccgt tcgaccgggt
     1801 gctgagcggc gagcaggcgg acgaagcgtt ccaccggctc acaggctgat tcgcgcgccc
     1861 ggcccggggt aggaccggga catggccgaa ctcagcgatc agctcaccaa gtacctgacc
     1921 gacgcctact cgatcgagca gcaggcgctc gctcagctcc gcaccgctcc cgacctcgtc
     1981 ggcgatcccg agctcgcgcg catcttcagc gagcacctga ccgagaccga gggccaggag
     2041 gagcggatcg gggcgctgct cgaagagcgc ggcgcgtcgc cctcgcgcat caaggacatg
     2101 gtgatggcgg cgggcggcaa ggcgttcgtg ctgttcgccc gctcccagcc ggacacgacc
     2161 ggcaagctca ccgcccacgc gcactcctac gaggcgctcg aggaggccag ctacgagatg
     2221 ctgctcggcg tggccgagcg ggccggcgag gacgaggtcg tggacgcggc gcggtcgatc
     2281 cgcgaccagg agacgcggat gcgcgagcgg ctcgctggag ccttcgacgc cgcggccgag
     2341 cagtcgctcc gcgaggtcga ccccgacgac ctcgaccagc agctcaccaa gtacctcgcc
     2401 gacgcgcacg ccatcgaggc gcaggcgatc cagctactcc agcgcggtcc caagatcggc
     2461 ggcgtgccgg agctcgagca gctctacgag cgccacctcg agcagtcgcg cgaccagcag
     2521 cgccgcgtgg aggagcgcct cgacgcgctc ggcggctcgc ccaaccggct ccaggacgcg
     2581 gcgatgcggc tcggcgcgct caactggggc gccttcttcc aggcgcagcc cgacaccccc
     2641 ggcaagctcg ccgcgttcgc ctacgcattc gagcacctcg agatcggcgg ctacgagctg
     2701 ctcaagcgcg tggcggcccg ggcgggcgac caggagaccg tcgcgatggc ggactcgatc
     2761 atcgtcgagg agcgggcgat ggccggcagc gtgcaggcgc tgttcgcgaa ggcgctcgac
     2821 gcctcgctcg acgcgcaggg cgtcggcggc ggcgccggtt agactgccgc ctccgtggac
     2881 accagcaaga tcggcaagca gtacccaccc ttcgagtacg aggtcgggcg cgagaagatc
     2941 cgcgagtacg cgaacgcggt gggcgagacg aaccccgtcc accacgaccg cgccgccgcg
     3001 caggccgccg gctaccgcga cgtggtggcg ccgccgatgt tcgtcgtcgt ctactccgcc
     3061 ggcgcgatgg gccccgcgat cctcgacccc gaggtcggca tcaacctgat gctcatggtc
     3121 cacggcggcc aggagttcat ctggggtgag cccgtctgcg ccggtgacac cgtcaccacg
     3181 acgggcagcg tgaaggacat gtacgagaag gacgggcgga agttctacgt gttcgagtcg
     3241 gtctccacga accaggacgg ccaggagacc gcgcgcgcaa cgtggacgaa catcgtgcga
     3301 ggaggctgag gtggacgcag gcgaccagat ccccgagctg cgggtcacgc cggacaagta
     3361 cctgccgcac cgctacgcgg gcgcgtcggg cgacttcaac ccgatccaca tcgacccgga
     3421 gttcgcgacc aacgtggggc tgcccggcac gatcctgcac gggctctaca gcatgtccca
     3481 ggtcgcccgc gcgcagaccg cggcggccgg gggcgagccg cgcagcctga agcggctcgc
     3541 ggtgcagttc cggggcatgg gcttccccga gcaggagatc gttgtgaccg ggaccgtcag
     3601 ggaggttcgc gacggccgcg ccgtgatcga caccgaggcc gcgcagggcg ataaccggat
     3661 catccggaac gccgaggccg agatcgaagt ctcggagtag acgcgctccg cgaaaataga
     3721 cgtgtggact tgaccgcacg tcaatccgag ctgctccgcc gcctcgtcga ggcgcacgtc
     3781 tcgctgggcc agccggtcgg gtcgaagtgg ctcgccgaga actgcgacat gccgtgggga
     3841 ccgtccacga tccgtgccga gctggcgcgg ctcgaggagc tcgggctgct ccagcacccg
     3901 cacacctccg cagggcgcgt gcccaccgac cacggctacc ggcagtacgc cgacgcgctg
     3961 ctggccggtg ggaccacggc cgagctcgcg gcgccggtcg tccgcctgcc cgcggtgcgc
     4021 gccgaggtgg acgaggcgat gcgcgcgacc accgagcagc tctctcaggt gaccaacctc
     4081 ctggcgatcg tctccgcgcc ggcgctcggc accacgacga tcaagcacgt cgaggtgctc
     4141 ctgctccagc cgcaggtggc aatggtggtg gtgatcacct cgaccggcgg ggtcaccaag
     4201 cgagtggcga gctacaggcg gccggtggac ccgggcatcg tcaactgggc gcacagctac
     4261 ctcaacgagg tgctcggcgg gatggcggtg ggcgcgcgga tggtgcactc gaagctgcgc
     4321 gacccggagc tctcggacgc cgagcgcgag ttcctcgcca cgctcgagcc ggccttcagc
     4381 gagctcgacg agagcgcgcc cgacgacctc tacgtgggcg gcgcggcgcg gctgatgtcc
     4441 gaggatcggc tccaggagct ctcccagatc aacgacctga tgaaggtgct ggagcgccgc
     4501 gtggtgctgc tgtcgatgct gcgcagcgcg ctcgaggagc cgcgcgtgta cctgcggatc
     4561 ggcaccgaga acgagacgcc ggagctgcgc tcgtggacgg tcgtggccgc gaactacggc
     4621 atcgcgcggc gcaacctcgg caccgtgagc gtcgtgggcc cgacccggat ggactacccg
     4681 accgcgatcg cgtcggtgcg acaggcagcc gccgagctgt cgcgcttcgt cggcgacctc
     4741 tacgacgaat gaagcgcgac tactacgagg tcctgggcgt ctcgcgcggc gcggaggagc
     4801 gcgagatcaa gaaggcgttc cgccggctcg cgcgcgagct gcatcccgac gtgaacaagc
     4861 acgaccccga cgcggaggag aagttcaagg aggcggccga ggcgtacgag gtgctcaccg
     4921 actcggagcg ccgctcggtc tacgaccgct acggccacga cggcctgcag tcgagcgggt
     4981 tctcctcgaa cttctccgga ttcggctcgt tcggcgacat cttcgacgcg ttcttcggag
     5041 gcgactcgcc gttcggcggt gggatgcgca gcggggccag ccagggccgc gacgtggccg
     5101 tggcggtcga gatcacgctc gaggacgcgg cgcgcggcgc gagcgtggag gtgggctacg
     5161 acgcggtcga gatgtgcgag cactgccgcg gcaacggggc ggagcccggc acgccgatcg
     5221 agacctgccc gcgctgcgag ggcgccggga cgctgcgcgc ggtgcagcgc acggcgttcg
     5281 ggcagctcgt gcgccaggcc gtgtgcgaca cctgcggcgg agatggcaag ctcgccaagg
     5341 agccgtgccg tgagtgccgc ggccgcggcc gccaggcgtc gcgcaagacc atcagcgtgg
     5401 acgtgcccgc cgggatcgcc gacgagcagc gcatccggct cacagggcgc ggccacgccg
     5461 gcgagcgcgg cggcccggcc ggcgatctct acgtgctggt ccgtgtgcgc gaggacgagc
     5521 gcttcgtgcg cgacggcaac gacctggtct cgcaggtctc gatctccgcc cccgaggccg
     5581 ccctcggcac caccgtcgcg gtcgacaccc tcgacggtcc cgaggagatc gaggtcgagg
     5641 ccggcacgca gccgggcacc gtcgtcacgc tccggggacg cgggatgccg tcgctgcggc
     5701 acggacagcg gggggaccag cgggtgctgg tgaacgtgct gatcccgcag cggctcagcg
     5761 ctcagcagcg cgagatgctc gagcgcttcc gcgagacgct caccgacgag aacctccgcg
     5821 gcgccgagga cgacgggttc ttcgcgcgtg tgcggcggtc gttccgctga tcctgtcttg
     5881 atccgcctcg cgctccgcgc ctccgtcgag gacgccgaga gggtgctcgc agcacttata
     5941 gagctggctc cgagtggggt cgagcaggtc gatggtcccg ggtgggtcga gtacgccgtg
     6001 tacggcatgc ccggggagct cccctcgttg ccggagggtc ccgcggaggt cggcggcgcg
     6061 cgcgtgacgg tgcgcggtga accggtgccc gacgactggg ccgagcgctg gaagcgcttc
     6121 cacgtgccgg tgctggtcgg cgggcggctc tgggtgcggc cgccgtggga gcaggaggcg
     6181 gtgcggcccg gcgtgcacga gatcgtgatc gacccgggtc aggcgttcgg caccggcacg
     6241 cacccgacca cgcggctctg cctcgagctg atgctcgagc tcgacccgcg cggctcgttc
     6301 gcggacctcg gctgcgggtc gggggtgctg tcgatcgcgg cggcgaagct gggcttccgg
     6361 ccggtgtgcg cgtacgacag cgaccgcgcc gccgtggcgg cgaccctcag taacgcccgg
     6421 gacaacggcg tggctttgga tcgcatcgag cggttcgatc tccgcggggc ggcgcctcct
     6481 gtggccgacg tcgtcgcggc caatctgatg cgcccgctgc tgctccaggt acggcaatca
     6541 ctcagggagc tgccccgggt gctcgtcctg tccggcctcc tcgaggccga ggcggacgag
     6601 gtggtcgccg cgtacgcgcc gctcgtggag cgccgccggc tcgtcgaccg cggctgggcc
     6661 gcggtccttc tcgcccactg aacagttaat gaccctatgg ggtcatcaag tgttcagaac
     6721 tactccggaa cctccgtcgc cagcgcgccg atgacggtct ggagccaggc gcgctcgtcg
     6781 gggctcacct gggacatgcg ccggcgcgcc caggcggaga cctgcgttcg cccgccgacc
     6841 gcgagcaccg ggccggccag cgcgtagcgg ccgtcctcga gcacgaagag cccgcccggg
     6901 cggtcgcgcc gcacgcggag ctggtccacg acccgccggc cgccgatcgc gccgggctgc
     6961 gcggggtcgc cgtcggcgtc gcgccagggg cgcgcggtca tgatcggctc gcgtccctgc
     7021 gggctgcgcg gcgcgcgggg ccgggggccg atcggaccgt cgcgccagcg gcgcgtgagc
     7081 gccggcacct gcgcggccgc ctcgtggcgc atcagcacgt gcacgcggtc ggccgcgcgc
     7141 agccgggtcg agccacgcgg cgggatcgcc tggtcctcgc gcacgatcac gttgaccacg
     7201 gcctcgcgcg gcagccccag gtcgcgcacg cgcgcgccga cgatcgcgtc ctcggcgccg
     7261 accgggtact ccagcacctc ggcccctagc cgccggatcg tgccggcctc ggcgagcgga
     7321 ctcggcagcg ccggctggtt cgtggtgagg cccaggcgct tggcgagcgg ctcgaaggtc
     7381 gtcccctgga gcagcgtcga gatcacgacg aggaagaaga cgatgttgaa gaactcgcca
     7441 ctgcccggca cgtcctcgat caccgggaag gtggcgagca cgacgggcac ggcgccgcgc
     7501 agcccggccc agccgagcac caggcgctcg cgcgaggtga accggtcgat cgccgtgccg
     7561 atcacggtgc ccaccggtcg cgcgaccagc acgagcacga gcgccagcac ggtgccctcg
     7621 aaccacacgt cgcccagctc gctcgggaac acgagcaggc cgagcgcaag gaacatcgcg
     7681 atctgcgcca cccaggccat gccctggtgg aacgtgatga tcgtgcgctt ggccgggatg
     7741 cccgccgtgc cgaggatcaa cccggacagg tagaccgcga ggaagccgga cccgtggatg
     7801 aactccgcgg cgccgaacgc cagccccgcg gtggcgagcg aggccaccgg gtagagcccc
     7861 gccgtctcga gctgagcgcg gcggaaggcg tagacggcgc cggagccgat cgcgactccg
     7921 ataacgacgc cgatcccgag ctgctgcgcg aacaggccga gcatgtcgag cagtccgtag
     7981 ccgggctcct cgatccactc gatgaagccg agcacgagga ggatcgcgac cgggtcgttc
     8041 agccccgact cgccctcgag ggtgcgggcc agcttgcgct cgagcgtcga gttgcgcagc
     8101 aggccgaaga tcgcggcgcc gtcggtggcg gagacgatcg cgccgagcag gagtcc
//
LOCUS       DASVPL010000019         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_1796, whole genome shotgun sequence.
ACCESSION   DASVPL010000019 DASVPL010000000
VERSION     DASVPL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VF517_00995"
     CDS             <1..618
                     /locus_tag="VF517_00995"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8051540.1"
                     /translation="SRDYVFGDKHELSSVEEQGAAARAVVDAVLGDDFRRFLSSLARR
                     ELFVDPAFHGGGFHQGGDGSFLDPHVDFNVHPLHPDWLRVLNVLLYLNRDWRPEYGGS
                     LELRNGLTGETGAVEPLFNRAVVMLTADHTYHGYPRMSLPPGVTRKSVAVYAYQLVPA
                     NSVRARTTGWAPDDAGWAKRLLARRYDTLVRVKTRVAGSATAKNR"
     gene            713..1114
                     /locus_tag="VF517_01000"
     CDS             713..1114
                     /locus_tag="VF517_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8051541.1"
                     /translation="MRFMLLQDYGGVESDTEPMSRWTQDEVKAHIDFQRRLNAELREH
                     GELIAAEALTAPDQAKTVVSDGSAAPVVTDGPYPESKELLAGYRMVDVESVERAIEIA
                     ARASAAPGQGGAPIRQRIEVREVMSPLGDDV"
     gene            1111..>1991
                     /locus_tag="VF517_01005"
     CDS             1111..>1991
                     /locus_tag="VF517_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8051542.1"
                     /translation="MSRPGPRVEDLLRDAAPQVLAAVVRRFRRFADSEDAVQEAMIAA
                     AEQWPADGVPDNPVGWLVHVASRRMTDRARSETARRRREEAALAQDGVPPEPAAEHDD
                     TLALMFMCCHPELTAPSAIALTLRAVGGLTTGEIARAFLVPEATMAQRISRAKRTLSD
                     ADEPFRMPAGAEWDERLRSVLHVLYLIFNEGYLASGGPALARSELSNDAIRLARTVRL
                     ALPDHAEVAGLLALMLLTDARRPARTDAGGSLVPLAEQDRTLWRREPIAEGVALLAAT
                     LDGAALGEYQLQAAIAAV"
BASE COUNT          272 a          733 c          749 g          237 t
ORIGIN      
        1 agccgcgact acgtgttcgg cgacaagcac gagctgtcgt cggtggagga gcagggcgcg
       61 gcggcgcgtg cggtggtcga cgcggtgctc ggcgacgact tccggcgctt cctctcctcg
      121 ctcgcccgtc gcgagctgtt cgtggacccg gcgttccacg gcggcggctt ccaccagggc
      181 ggcgacggga gcttcctcga cccgcacgtt gacttcaacg tgcacccgct gcaccccgac
      241 tggctgcgcg tgctcaacgt gctgctgtac ctgaaccgcg actggcgccc cgagtacggc
      301 ggcagcctgg agctgcgcaa cgggctgacc ggcgagacgg gcgcggtcga gccgctgttc
      361 aaccgcgcgg tcgtgatgct gaccgcggac cacacctacc acggctaccc gcggatgtcg
      421 ctgccgccgg gagtgacgcg caagagcgtc gcggtctacg cgtaccagct cgtcccggcc
      481 aacagcgtgc gggcgcggac gacgggttgg gccccggacg acgcgggctg ggccaagcgg
      541 ctgctcgcgc gccgctacga cacgctcgtg cgggtcaaga cgcgcgtcgc cggcagcgcg
      601 acggcgaaga accgctagcc gcaacgcgcg cgcccccgcc gtgtagagaa tcctctcgcg
      661 gctccgacac ccccgaacgc gccgcgaggg cgcaccgacc aaggaggcaa ccatgaggtt
      721 catgctgctg caggactacg gcggggtcga gtcggacacc gagccgatga gccgctggac
      781 tcaggacgag gtcaaagcgc acatcgactt ccagcgccgg ctgaacgccg agctgcgcga
      841 gcacggcgag ctgatcgccg ccgaggcgct gaccgcgccc gaccaggcga agaccgtcgt
      901 ctccgacggc agcgccgcgc cggtggtcac ggacggcccg tacccggagt cgaaggagct
      961 cctggccggc taccggatgg tcgacgtcga gtcggtcgag cgggcgatcg agatcgcggc
     1021 gcgggcgtcg gccgcacccg gccagggcgg cgcgccgatc cggcagcgga tcgaggtccg
     1081 cgaggtgatg agcccgctcg gcgacgacgt gtgagccggc cgggaccgcg cgtggaggac
     1141 ctgctgcgcg acgccgcgcc gcaggtcctc gccgcggtcg tccgccgctt ccgccgcttc
     1201 gcggactccg aggacgccgt gcaggaggcg atgatcgccg ccgccgagca gtggcccgcg
     1261 gacggggtgc ccgacaaccc cgtcggctgg ctggtgcacg tcgcgtcgcg gcggatgacg
     1321 gaccgcgccc gcagcgagac ggcgcgccgc cgccgcgagg aggccgccct ggcgcaggac
     1381 ggggtcccgc cggagccggc cgccgagcac gacgacacgc tggccctgat gttcatgtgc
     1441 tgccacccgg agctcacggc gccgtcggcg atcgccctga ccctgcgggc ggtcggcggg
     1501 ctcacgacgg gcgagatcgc gcgggcgttc ctcgtgcccg aggcgaccat ggcgcagcgc
     1561 atcagccgcg ccaagcggac gctctccgac gcggacgagc ccttccgcat gccggccgga
     1621 gccgagtggg acgagcgcct gcgctccgtg ctccacgtgc tctacctgat cttcaacgag
     1681 ggctatctgg cgagcggcgg ccccgcgctc gcgcgcagcg agctgtcgaa cgacgcgatc
     1741 cgcctggcgc ggaccgtccg cctcgcgctg cccgaccacg cggaggtggc ggggctgctc
     1801 gcgctgatgc tgctcaccga cgctcgccgg cccgcgcgga ccgacgccgg cggcagcctg
     1861 gtcccgctgg ccgagcagga ccggacgctg tggcggcgcg agccgatcgc ggagggcgtc
     1921 gcgctgctcg cggcgacgct cgacggagcc gcgctgggcg agtaccagct gcaggccgcg
     1981 atcgcggccg t
//
LOCUS       DASVPL010000020         9361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_158006, whole genome shotgun sequence.
ACCESSION   DASVPL010000020 DASVPL010000000
VERSION     DASVPL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158006"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VF517_01010"
     CDS             <1..180
                     /locus_tag="VF517_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051543.1"
                     /translation="GLITPVDAGVARPLIEGLSTRTVVTDPSGTEPFDVEPTPLREAL
                     RRACAEEREAVAAAA"
     gene            complement(184..435)
                     /locus_tag="VF517_01015"
     CDS             complement(184..435)
                     /locus_tag="VF517_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051544.1"
                     /translation="MARKKRDGTSEFVESLMDTSLYSMGAFFSDQHPDLVEEVIAQSE
                     AIEDLGLRGYAEETDLSVEEVFETLLTGLAVRYYKAVAA"
     gene            471..1235
                     /locus_tag="VF517_01020"
     CDS             471..1235
                     /locus_tag="VF517_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX8051545.1"
                     /translation="MAHRDEIVAHLDELLDARAWDDYGPNGLQVTGREEVELVVTGVS
                     AHRELFERAAAAGAGMVLCHHGIFWRSTPQAVGPQMKGRLKALFDADMSLAAYHLPLD
                     AHEEVGNNVLICAELGLRRGERFSEAAGRPIGFVGHADATLTLDELARRCAAAFGREP
                     LVLPGGEPPGEVRRVGIVSGGGGSALADAAALGLDALVTGEPEEPAMADAAETGVSFL
                     ACGHYATETFGIRRLGEVLAERFGVRHTFIEVRNPV"
     gene            1607..3397
                     /locus_tag="VF517_01025"
     CDS             1607..3397
                     /locus_tag="VF517_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8051546.1"
                     /translation="MEAATGVSALEMGTHTKTIAAEVTAAVEKHSGAVAQMHKSGDEW
                     VTTTYEEMGVAAKEIALGLLDLGIGAQDRVAILSHTRPEWTWANFGTLSAGATSVSIY
                     QTNSPEECHYVLEHSESVAVFAEDEEQLAKLREIRDDLPALKHVIAFEPGSETDGVIS
                     LDELRERGRGGSDEDWQRAAEAVRPEDPCQFIYTSGTTGPPKGCVITHANYVAMRDMV
                     LPHGTVVPEDIVYLYLPLAHSFALLIQFISLDVGGTVAYWQKDPLKIVPDLQEVRPHY
                     FPSVPRIFEKIYTLATSNAPDPEKLQQAVKLGMKVREMQARGEDVPAELQEPFDQAEE
                     ALYKNVRAIFGGRVRQGVSGAAPIATEVLEFFYACGIPVFEGYGMTETSTAATHQTLT
                     EFRFGSVGRLFEPIEGKIAEDGELLLRGPNIFKGYYKNEEATAEALEDGWLHTGDLAR
                     IDEDGYVYITGRKKDIIITAGGKNITPANLENGLKQNRYISQAVVLGDRKPYLVALIT
                     LDPDELPGFAEQHGLKPEDVADSEEMRAVVQEAIDEVNSQVGRVEQIKKFKILAHDLS
                     LATGELTPTMKVKRNVVAEKFAGEVENLYA"
     gene            3451..3927
                     /locus_tag="VF517_01030"
     CDS             3451..3927
                     /locus_tag="VF517_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051547.1"
                     /translation="MASNDPSDTGGLFIGRRPGTAPLRYRGDTPEAGQARQRADRGFA
                     ALLLVIETLLCVSLWGPQPIGWLWVGSRVNYWTDSVSAGIVTAFLGMLLSLFVTLMIA
                     KRVDHAWKLVRRASGIRQEKGALERIFLVSAVVVGIAFTIWFILIEGPGPSLAPRD"
     gene            3952..5079
                     /locus_tag="VF517_01035"
     CDS             3952..5079
                     /locus_tag="VF517_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8051548.1"
                     /translation="MGLLDYYRQWDDVDHETINSELRARRAREKALALEHVPTLDLSS
                     TEWPDFPNAEIMNAAIYAARGRVNGYPDRHATQIRRQLAERHDVEPEQIVFGNGAGEL
                     IQATALTLMRSGDELITPWPSYPLYPLVAQRAGGRPVAIDLRGGNADLDAVRAAVTPD
                     TRVVVICNPNDPSGTYVSSDELGSLASSLPEHVHLLVDEAYIEFQDVEPRDSVLRLVE
                     AFPRMLVFRTFSKIYGLSGLRAGYAVGSRAAGELLSSIGPALGVNVLTQAGICYALQL
                     DDRDIERRRELVIEQRRRVHDELYDLPVDAPASQANFVWLHAAGMSGAELAARLEEKR
                     VLVAPGGPLGADDCVRASIRYSGATGRLLSALREAVGAEPS"
     gene            complement(5099..5947)
                     /locus_tag="VF517_01040"
     CDS             complement(5099..5947)
                     /locus_tag="VF517_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX8051549.1"
                     /translation="MKGKATAAMVIAGILFGLVVGALVAGDDDGDDDKSTPKRTATAS
                     LGAGGAKPPPGPVDKTERPPLPSRAAIAREPKGSGPAYVGVYLYPPENLRVRAGSPSD
                     TSLIFEWNERGYFDTFQVYRWSGSTWLRVREKVTNTSYTDKELTPGTQYWYTVCAVRG
                     APNAGPRGGRPPDVVECAPGVATGITATQPPPTPTALSAFPSGGKIRFQWTQPCFNYP
                     CPAIRSFEVYRNSSRGELRSWQLDGNTRFVEDDRVQPGVAYIYNVCATGNLGRACAPK
                     WVQAQL"
     gene            complement(6003..7268)
                     /locus_tag="VF517_01045"
     CDS             complement(6003..7268)
                     /locus_tag="VF517_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8051550.1"
                     /translation="MGKVVKLEQPEKRPAERAKPARRRGRRSKSALVLGGGGVTGGVY
                     EIGALRALDLLAVNRTINEFDVYVGTSAGSFVASLAANGVTPEEMMRVVNQQVPTPFR
                     DINMGTVLKLDYSELAKSLALMPLRSVGVVRTLLSNLRNVSAMDLALGFAEGLPPGIY
                     DGSGIERYLDEVLSDPDRVNDFRMLENELYLTATDLDTCERIVFGADGWDDVPISLAS
                     AASTALPMIYRPVEIKGRHLVDGGIRSTTNVDVAVEAGAKFVIVVNPLVPYVNDFQKL
                     VPTMTGSRVRRVADMGFPQIGYQAFKLLAHQRLHEAVRVWQEKYPGVDIILIEPDPND
                     ELMFDTNIMNFTRRVDIARHGFESVTLQLAKDYDRLRAVCARHGIEISATRVRKVIKH
                     FEREKEQTRAWRRILEQTTGALLRQSEDA"
     gene            7358..8599
                     /locus_tag="VF517_01050"
     CDS             7358..8599
                     /locus_tag="VF517_01050"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX8051551.1"
                     /translation="MPRARRPILLLALLLLALPAAPAAAQSERDKLRSVLTREMRNAA
                     SGSGAYVADAKTGTPLFAWKSGTRRTLASNTKLFTIAGALAAHGPDARLETDVLGVGA
                     IDPDTGTFPGDLYLRGAGDPSFGTSAYNRSQYSGGASIETLVDELRRAGLRRVRGAIV
                     GDETIWDRLRGNSYSGYHGSSAIGGPITALAFNHGRSSSGSFQQNPPVYAADRFAQAL
                     RDGGIKVDRAARAGATPSSAVELADVQSSPMARLTQITGVRSENWFAEMLVKGLSAQG
                     TTRAGAAAARGFARSLGSQVSMVDGSGLSASNRAAPREVADLLVGEYARPEYAAFFES
                     LPTAGVNGTLATRMRSGPARGRCHAKTGTLNAGHTALSGYCVTLGGDTIAFSILMNYA
                     SISAGRGQQDRMAQAIARYRG"
     gene            8642..>9361
                     /locus_tag="VF517_01055"
     CDS             8642..>9361
                     /locus_tag="VF517_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8051552.1"
                     /translation="MTIDQAVELLELKPPADLRDIQLARRALAKRWHPDRASAAQRHV
                     HERQMKSINSAADLLETRVTDAGPITAMDVRVSADVYRQQQREAGERAYAEAQEEPAT
                     RRAGTSAERSIVYRYVRSASFPEWGVGSIVDVRFSGEGDDIQRWAQVQFGTGVVLLPI
                     DNLTYVDFRQHEKDIERADRFLSAARDAVAEGKHDLAIKRLVYARNADPRRAEILSLL
                     AHEYLRDGRLKEAGRSVRDWIR"
BASE COUNT         1404 a         3334 c         3300 g         1323 t
ORIGIN      
        1 ggactgatca cgcccgtcga cgccggagtg gcgcgccctc tgatcgaggg cctctcgacc
       61 cgcacggtcg tgaccgaccc gtccggcacc gagccgttcg acgtcgagcc gactcccctg
      121 cgcgaggcgc tgcggcgcgc gtgcgcggag gagcgcgagg cggtcgcagc cgcggcgtga
      181 ccgctaggcc gccacggcct tgtagtacct gacggccagc ccggtcagga gggtctcgaa
      241 gacctcctcc accgagaggt ccgtctcctc ggcgtagccg cgcagcccga gatcctcgat
      301 cgcctcgctc tgggcgatca cctcctcgac caggtccggg tgctggtcgg agaagaacgc
      361 ccccatcgag tagaggctcg tgtccatcag ggactcgacg aactcgctcg tcccgtcgcg
      421 cttcttgcgg gccatcggcg ggcaggttac gcgtcgtcta cggtgcgcgc atggctcacc
      481 gcgacgagat cgtggcccac ctcgacgagc tgctcgacgc gcgtgcgtgg gacgactacg
      541 ggccgaacgg gctccaggtg accgggcgcg aggaggtcga gctggtggtg acgggcgtct
      601 ccgcccaccg agagctgttc gagcgagcgg ccgcggcggg cgcggggatg gtgctctgcc
      661 accacgggat cttctggcgc tcgacgccgc aggcggtcgg gccgcagatg aagggccgcc
      721 tgaaggcgct gttcgacgcc gacatgtcgc tcgccgccta ccacctcccg ctcgacgcgc
      781 acgaggaggt gggcaacaac gtgctgatct gcgccgagct cggcctgcgc cgcggcgagc
      841 gcttctcgga ggccgccggg cgcccgatcg gcttcgtcgg ccacgcggac gcaacgctca
      901 ccctcgacga gctcgcacgc cgctgcgccg ccgcgttcgg ccgcgagccg ctggtgctcc
      961 cgggtggcga gcccccgggc gaggtgcgcc gcgtcggcat cgtgtcgggc ggcggcggct
     1021 ccgcgctggc cgacgccgcg gcgctcggcc tcgacgcgct cgtcaccggc gagcccgagg
     1081 agcccgccat ggccgacgcc gccgagactg gcgtgagctt cctggcgtgc ggccactacg
     1141 ccaccgagac gttcgggatc cggcggctcg gcgaggtgct ggccgagcgg ttcggggtgc
     1201 gacacacctt catcgaggtc cgaaatccgg tctgatgtgc cgcatcggcg ttggaatcag
     1261 cgcaaaatgc agaggtcgga ttgcgacgtc cggcgcaagc ggtaacaatc cgagggccat
     1321 tcaccgacgg agggagacaa ctccatggcg atccacttga cgcccacaga gctcgcgcgc
     1381 gaggcaggta tgcatcggcg ggaggtgatc gtcaagtgca tggagttgca ggtcccgatc
     1441 ttccagggcc ggatcgacaa gacgctgttc ctctcggcac tcgccgaggc ccggcagcag
     1501 acgggcacgc gcgacgcgca gctggccggc gcctgacccg ctagcccccc gcccccctcg
     1561 cgtccctaga atcccccgcg caaccgacgc cggaggggac atctcgatgg aggccgcgac
     1621 gggcgtgagc gccctcgaaa tggggacgca cacgaagacg atcgccgccg aggtgaccgc
     1681 cgcggtagag aagcactccg gcgcggtcgc ccagatgcac aagtccggcg acgagtgggt
     1741 cacgacgacc tacgaggaga tgggcgtcgc cgcgaaggag atcgcgctcg gcctgctcga
     1801 cctgggcatc ggggcgcagg accgcgtcgc catcctcagc cacacgcgcc ccgagtggac
     1861 ctgggccaac ttcggcacgc tcagcgccgg cgcgacatcg gtctcgatct accagaccaa
     1921 ctcgccggag gagtgccact acgtgctcga gcactccgag tcggtggcgg tgttcgccga
     1981 ggacgaggag cagctcgcga agctacgcga gatccgcgac gacctgcccg cgctcaagca
     2041 cgtcatcgcc ttcgagcccg gatccgagac cgacggcgtg atctcgctcg acgagctgcg
     2101 cgagcgcggc cgcggcggaa gcgacgagga ctggcagcgc gccgcggagg ccgtccgccc
     2161 cgaggacccg tgccagttca tctacacgtc cggcaccacg ggcccgccga agggctgcgt
     2221 gatcacccac gccaactacg tcgcgatgcg cgacatggtg ctgccgcacg gcacggtcgt
     2281 gccggaagac atcgtctacc tctacctgcc gctcgcgcac tcgttcgcgc tgctgatcca
     2341 gttcatctcg ctcgacgtgg gcggcacggt cgcctactgg cagaaggacc cgctgaagat
     2401 cgtgccggac ctacaggagg tgcggccgca ctacttcccg tcggtgccga ggatcttcga
     2461 gaagatctac acgctcgcca ccagcaacgc gcccgatccg gagaagctcc agcaggccgt
     2521 caagctgggc atgaaggtgc gcgagatgca ggcgcgcggc gaggacgtcc cggcggagct
     2581 ccaggagcct ttcgaccagg ccgaggaggc gctctacaag aacgtgcgcg cgatcttcgg
     2641 gggccgggtc cggcagggcg tgagcggcgc cgcgccgatc gctaccgagg tgctcgagtt
     2701 cttctacgcc tgtggcatcc ccgtcttcga gggctacggc atgacggaga cgtcgaccgc
     2761 cgccacgcac cagaccctca ccgagttccg cttcgggtcg gtcggcaggt tgttcgagcc
     2821 gatcgagggg aagatcgccg aggacggcga gctgctcctg cgcggcccga acatcttcaa
     2881 gggctactac aagaacgagg aagcgaccgc cgaggcgctc gaggacggct ggctccacac
     2941 cggcgacctc gcgcgcatcg acgaggacgg gtacgtctac atcaccggcc gcaagaagga
     3001 catcatcatc acggcgggcg gcaagaacat caccccagcc aacctcgaga acgggctcaa
     3061 gcagaaccgc tacatctcgc aggccgtggt gctgggcgac cgcaagccgt acctggtggc
     3121 gctgatcacg ctcgaccccg acgagctgcc cggcttcgcg gagcagcacg gcctgaagcc
     3181 cgaggacgtg gcggactccg aggagatgcg cgccgtcgtc caggaggcga tcgacgaggt
     3241 gaactcgcag gtggggcggg tcgagcagat caagaagttc aagatcctcg cgcacgacct
     3301 ctcgctggcg accggtgagc tgacgcccac gatgaaggtc aagcggaacg tcgtggcgga
     3361 gaagttcgcc ggcgaggtcg agaacctcta cgcgtgacaa ggcgcacgcg gtagcgtccg
     3421 catcccccga tccgggtacc cccacccacc ttggcgagca acgacccctc agataccggc
     3481 ggccttttca tcgggcgccg gcccggaacc gcgccgctcc gctaccgcgg ggacacgccg
     3541 gaggccgggc aggcgcgcca gcgcgcggac cgcgggttcg ccgccctgct gctggtgatc
     3601 gagaccctgc tgtgcgtctc gctctgggga ccgcagccga tcgggtggct gtgggtcggc
     3661 tcgcgcgtga actactggac cgactcggtc tcggcaggca tcgtcacggc gttcctcggg
     3721 atgctgctct cgctgttcgt cacgctgatg atcgcgaagc gggtcgacca cgcgtggaag
     3781 ctcgtgcgcc gcgcctccgg catccggcag gagaagggag ccctcgagcg gatcttcctc
     3841 gtcagcgcgg tggtcgtcgg catcgcgttc accatctggt tcatcctcat cgaggggccc
     3901 ggaccgtcgc tcgcaccgcg ggactagcgg gacgccgggg cggtagggga catggggctg
     3961 ctcgactact accgccagtg ggacgacgtc gaccacgaga cgatcaacag cgagctgcgc
     4021 gcccgccgcg cgcgcgagaa ggcgctcgcc ctcgagcacg tcccgaccct cgacctgtcg
     4081 agcaccgagt ggccggactt cccgaacgcc gagatcatga acgcggccat ctacgccgcg
     4141 cgcgggcggg tgaacggcta cccggaccgg cacgccacgc agatccggcg ccagctcgcc
     4201 gagcgccacg acgtcgagcc cgagcagatc gtgttcggca acggtgccgg tgagctgatc
     4261 caggccaccg cgctcacgct catgcggagc ggcgacgagc tgatcacgcc gtggccgagc
     4321 tatccgctct acccgctggt ggcgcagcgc gccgggggcc gcccggtggc gatcgacctg
     4381 cgcggcggga acgccgacct cgatgccgtg cgtgccgcgg tcacgcccga cacgcgggtg
     4441 gtggtcatct gcaacccgaa cgacccgagc ggcacgtacg tcagctccga cgagcttggc
     4501 tcgctcgcct cctcgctgcc cgagcacgtg cacctgctcg tggacgaggc ctacatcgag
     4561 ttccaggacg tcgagccgcg cgactcggtg ctgcggctgg tcgaggcctt cccccgcatg
     4621 ctcgtgttcc ggaccttctc gaagatctac gggctctccg ggctgcgggc cggctacgcg
     4681 gtcgggtcgc gcgcggcggg cgagctgctc agctcgatcg gcccggcgct cggcgtgaac
     4741 gtgctgaccc aggcgggcat ctgctacgcg ctccagctgg acgatcgcga catcgagagg
     4801 cgccgcgagc tggtgatcga gcagcgccgc cgcgtgcacg acgagctgta cgacctgccg
     4861 gtcgacgcac cggcgagcca ggccaacttc gtctggctgc acgcggccgg gatgtccggc
     4921 gcggagctgg cggcgcgact cgaggagaag cgcgtgctgg tggcgcccgg cggcccgctc
     4981 ggagccgacg actgcgttcg cgcgtcgatc cgctactcag gcgccacggg ccggctgctg
     5041 agcgcgctgc gcgaggcggt cggcgccgag ccgagctagc gcccgggtcg gctcccggtc
     5101 agagctgcgc ctgaacccac ttcggcgcgc aggcgcggcc gaggttgccg gtcgcacaga
     5161 cgttgtagat gtaggccacg cccggctgca cgcggtcgtc ctcgacgaag cgggtgttgc
     5221 cgtcgagctg ccaggagcgg agctcgccgc gcgagctgtt gcgatacacc tcgaagctgc
     5281 ggatcgccgg acaggggtag ttgaagcagg gctgggtcca ctggaagcgg atcttcccgc
     5341 ccgacgggaa cgccgagagc gcggtgggtg tgggcggagg ctgggtggcg gtgatacccg
     5401 tcgccacacc cggtgcgcac tcgaccacgt cgggcggccg gccgccgcgc ggccccgcgt
     5461 tgggtgcccc acggacggcg cacaccgtgt accagtactg ggtaccgggt gtcagctcct
     5521 tgtccgtgta gctcgtgttg gtcaccttct cgcggacgcg cagccacgtg ctcccgctcc
     5581 agcggtagac ctggaaggtg tcgaaatagc ccctctcgtt ccactcgaag atcagcgagg
     5641 tgtccgacgg gctgcccgcg cgcaccctca ggttctcggg cggatagagg tagacgccga
     5701 cgtaggccgg accgctgccc ttcggctcgc gcgcgatcgc cgcgcggctc ggcagcggcg
     5761 ggcgctccgt cttgtcgacc ggcccgggcg gcggcttggc tccgcccgcc ccgagcgagg
     5821 cggtcgctgt ccgcttgggc gtgctcttgt cgtcgtcgcc gtcgtcgtca ccggccacga
     5881 gcgcccccac tacgaggcca aacaggatgc ccgcaatcac catcgccgct gtcgccttgc
     5941 ctttcatgag ccgtgctttt ctccacaccg ctcaggattc ctttcagcgg gtgaaggagc
     6001 tgctaggcgt cctcggactg gcgcagcagt gcgccggtcg tctgctcgag gatccggcgc
     6061 caggcgcggg tctgctcctt ctcacgctcg aagtgcttga tgaccttgcg cacgcgcgtg
     6121 gccgagatct cgatcccgtg gcgggcgcat accgcgcgca ggcggtcgta gtccttggcg
     6181 agttggaggg tgaccgactc gaagccgtgg cgcgcgatgt cgacgcggcg cgtgaagttc
     6241 atgatgttcg tgtcgaacat cagctcgtcg ttcgggtcgg gctcgatcag gatgatgtcc
     6301 acgcccgggt acttctcctg ccagacgcgg accgcctcgt gcagccgctg gtgggcgagc
     6361 agcttgaacg cctggtagcc gatctgcggg aagcccatgt ccgccacgcg gcgcacgcgg
     6421 ctgccggtca tcgtcgggac gagcttctgg aagtcgttca cgtacggcac gagcgggttc
     6481 acgacgatca cgaacttcgc ccccgcctcc accgcgacgt ccacgttcgt ggtcgagcga
     6541 atgccgccgt cgaccaggtg gcggcccttg atctcgaccg ggcggtagat catcggcagc
     6601 gccgtcgagg cggcggaggc gagcgagatc ggcacgtcgt cccagccgtc ggcgccgaag
     6661 acgatccgct cgcaggtgtc gaggtccgtg gccgtcaggt agagctcgtt ctcgagcatc
     6721 cggaagtcgt tgacgcggtc cgggtcgctc agaacctcgt cgaggtagcg ctcgatcccc
     6781 gagccgtcgt agatgccggg cggcagaccc tcggcgaagc cgagcgccag gtccatggcc
     6841 gagacgttgc ggaggttcga cagcagcgtg cgcacgacgc ccaccgagcg cagcggcatc
     6901 agcgcgaggc tcttggccag ctcgctgtag tcgagcttga ggaccgtgcc catgttgatg
     6961 tcgcggaacg gcgtgggcac ctgctggttg acgacccgca tcatctcctc gggcgtcacg
     7021 ccgttggccg cgaggctcgc cacgaacgag cccgcgctgg tgccgacgta cacgtcgaac
     7081 tcgttgatcg tgcggttcac cgcgagcagg tcgagcgcgc gcagggcgcc gatctcgtag
     7141 accccgccgg tgactccgcc gccgcccagg acgagcgcgg acttggaccg ccgcccgcgc
     7201 cggcgcgccg gcttggcgcg ttccgcgggc cgcttctcgg gctgctcgag ctttacgacc
     7261 ttgcccatcg accggcgagt atggcagcgg ccgaggcgtc cggacccgga ccgattccgc
     7321 aaccggcggc cgttatggtg gttctcccct gcgacgaatg ccccgcgccc gccgcccaat
     7381 cctcctgctg gccctgctgc tgctcgcgct gccggccgcg cccgcggccg cgcagagcga
     7441 gcgggacaag ttgaggagcg ttctcacgcg cgagatgcgc aatgccgcct ccggctccgg
     7501 cgcgtacgtg gcggacgcca agaccggcac ccctctgttc gcctggaagt cgggcaccag
     7561 gcgcacgctc gcctcgaaca cgaagctgtt caccatcgcc ggggcgctgg ccgcgcacgg
     7621 tccggatgcc aggctcgaga ccgacgtgct gggcgtgggc gccatcgacc cggacaccgg
     7681 cacgttcccc ggcgacctct acctgcgcgg cgccggcgac ccgagcttcg gcacaagcgc
     7741 ctacaaccgc tcgcagtact ccggcggcgc cagcatcgag acgctcgtcg acgagctccg
     7801 ccgcgccggg ctgcgccgcg tgcgcggcgc gatcgtgggc gacgagacga tctgggaccg
     7861 cctgcgcggc aactcgtact ccggctacca cggctcgtcg gcgatcggcg ggccgatcac
     7921 cgcgctcgcc ttcaaccacg gccgcagctc gtccgggagc ttccagcaga acccgccggt
     7981 ctacgccgcc gaccggttcg cccaggcgct gcgcgacggc gggatcaagg tggaccgcgc
     8041 ggcccgggca ggcgcgacgc cgagctccgc ggtcgagctc gccgacgtgc agtcctcgcc
     8101 gatggcccgc ctaacgcaga tcacaggcgt gcggtccgag aactggttcg cggagatgct
     8161 ggtgaagggg ctctcggcgc agggcaccac gcgcgccggc gccgctgcgg cccggggctt
     8221 cgcgcgctcg ctcgggtcgc aggtctcgat ggtcgacggc tcaggcctgt cggcctcgaa
     8281 cagggcggcg ccgcgcgagg tcgcggacct gctcgtgggc gagtacgccc ggcccgagta
     8341 cgccgcgttc ttcgagtcgc tccccaccgc cggcgtgaac ggcacgctcg ccaccaggat
     8401 gcgcagcggt ccggcacggg ggcgctgcca cgccaagacc ggcactctga acgcgggcca
     8461 caccgccctg tcgggctact gcgtcacgct cggcggcgac acgatcgcct tctcgatcct
     8521 catgaactac gcgagcatct ccgccgggcg cggccagcag gaccggatgg cgcaggccat
     8581 cgcgcgctac cgcggctagg cgggcgcccg cgcacccgcg ccgcgtccta ggcttcaggg
     8641 cgtgacgatc gaccaggcag tcgagctcct ggagctcaag ccgcccgccg acctgcgtga
     8701 catccagctc gcccgccgcg cgctcgcgaa gcgctggcac ccggaccgcg cctcggccgc
     8761 gcagcggcac gtgcacgagc ggcagatgaa gagcatcaac tccgcggccg acctgcttga
     8821 gacgcgggtg accgacgccg ggccgatcac cgcgatggac gtgcgcgtga gcgccgacgt
     8881 gtaccgccag cagcagcgcg aggccggcga gcgcgcctac gccgaggcgc aggaggagcc
     8941 ggccacacgg cgcgcgggca cctcggcgga gcgctcgatc gtgtaccgct acgtgcgctc
     9001 ggcgagcttc cccgagtggg gcgtgggcag catcgtggac gtgcgcttca gcggcgaggg
     9061 cgacgacatc cagcgctggg cgcaggtgca gttcggaacg ggcgtggtcc tgctgccgat
     9121 cgacaacctc acctacgtcg acttccgcca gcacgagaag gacatcgaac gcgccgaccg
     9181 gttcctctcc gccgcccgcg acgccgtggc cgagggcaag cacgacctgg cgatcaagcg
     9241 gctggtgtac gcgcgcaacg ccgacccgcg ccgggccgag atcctcagcc tgctcgccca
     9301 cgagtacctc cgcgacggcc ggctgaagga ggcgggccgc tccgtgcgcg actggatcag
     9361 g
//
LOCUS       DASVPL010000021        23935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_158017, whole genome shotgun sequence.
ACCESSION   DASVPL010000021 DASVPL010000000
VERSION     DASVPL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 23935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23935
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158017"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VF517_01060"
     CDS             complement(<1..934)
                     /locus_tag="VF517_01060"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8051553.1"
                     /translation="MTAAAPDPIRFAVAAEQAGARLDVVLGAHPEVGSRSAAERLIEA
                     DRVTVNGARRQKRHLVEAGDLIEARPEEIAPEGDADAAPHEVVFEDDHLLVVDKPAGV
                     VVHPAPGHPTGTLVEALAGRAAGGDDPYRPGIVHRLDRDTSGLLVVAKSDRVLRALQE
                     QLQRRELRREYLALVAGRPDARSGTIDVAIGRDRRNRTIHSPRTDTPRAARTHFSIER
                     ALAHTTLLRVRLETGRTHQIRVHLAEIGHPVCGDPDYGGSAYGAELGLTRQFLHSALL
                     GFTHPGTGELLACESKLPVDLLRALEAAAREPVSE"
     gene            complement(931..1473)
                     /gene="lspA"
                     /locus_tag="VF517_01065"
     CDS             complement(931..1473)
                     /gene="lspA"
                     /locus_tag="VF517_01065"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX8051554.1"
                     /translation="MREWRLAGVVAAAVVALDQVLKQAVVSGIPRGEERNVFFGIDLT
                     YVRNEGIAFGALGDGGALIVVLTAVALAFLIGYFALHARRPWLWLPVGAVAGGALGNL
                     ADRARDGSVIDYLDPALWPAFNLADAAIVIGILGLLYVVEGPRKPRGAAGEPRAGDAA
                     GEPRAGDAAGEARAGDAAGA"
     gene            complement(1479..1766)
                     /locus_tag="VF517_01070"
     CDS             complement(1479..1766)
                     /locus_tag="VF517_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX8051555.1"
                     /translation="MSRADVAGYIETLVIVYVILIFVRVLMSYITRMPYNVALDKVLT
                     FVRDVTDPYLNMWRRILPMARLGPAAIDLSPMVGTIVLIVVGNIVAALIRG"
     gene            complement(1763..2518)
                     /gene="proC"
                     /locus_tag="VF517_01075"
     CDS             complement(1763..2518)
                     /gene="proC"
                     /locus_tag="VF517_01075"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX8051556.1"
                     /translation="MRLGFIGAGNMASAIVRGLREPVLVSDLDRSRAEALASAVGGEA
                     LDSNSAVAAGADAVVLCHKPAQLEEVAGEIGAGARAVVSILGGVPLERVEAAYPDRPV
                     YRLMPSIPVEVGQGVVLYAPGRLAAQGPESELRGLLDRLGTVVDLPEKLIDAGMAVMS
                     CGPAFLALVVEALVDAGVKQGLTTDVAGRLAVETMAGTAAVLRESGGDTRGLRTRVTS
                     PGGSTARGLAELEAGGVRAAFDEAVAAASGAKS"
     gene            complement(2537..3070)
                     /gene="sepF"
                     /locus_tag="VF517_01080"
     CDS             complement(2537..3070)
                     /gene="sepF"
                     /locus_tag="VF517_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX8051557.1"
                     /translation="MALRDTWHKTLVYFGLEEEEDRYRDHDSEPQPEEEIEDRYRERP
                     NVRRLQSRRRRDDFDDIFADEPPRSARETRVLRPVSNGRAESVQVHLVVPKSFNDAQQ
                     IADQFKDDVPVILNLQGTDTDLSKRLIDFASGLTYALDGGMQRIADKVFLLTPRNVEV
                     SAEERARLIEKGFFNQS"
     gene            3126..3953
                     /locus_tag="VF517_01085"
     CDS             3126..3953
                     /locus_tag="VF517_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8051558.1"
                     /translation="MPPRLAAEESGSGPPVVLLHGLSATRRYVVHRSKALPRAGFRTI
                     AYDARGHGESEAPDDPDAYDYADLCADLAALMDEHGLDRAALAGVSMGAHTALRFALE
                     HPERVSAVVAITPGFTGEAATDTGPWDRMADGLERGGVEGFVSEWGARDVAERYREAA
                     LEHARQRLSQHRDLAAVARALRVVPRSEPFPGLSSLRAVEAPALVVGSRDGADPMHPL
                     AVAEAYAGELPDARLLVEDEGRPPLAWQGARLSKAIAQFLGGAGSGGAGAGAGGAAG"
     gene            complement(3950..4567)
                     /locus_tag="VF517_01090"
     CDS             complement(3950..4567)
                     /locus_tag="VF517_01090"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8051559.1"
                     /translation="MEAGAQRHGQDGGWARFYEKPQGFEFFLSNLHYHREFLAEIFEA
                     KPSKTLEAGSGPATMSVFLAMAGIEAVACDLDATVLERAGENAERWNTQLRLLQHDIF
                     ALSELDESFDVVFSQGVLEHYSDEDVRRLAQEALAAAPVFVFSVPSRWYGRQDFGDER
                     LLEGDDWARMLDGVGTVRTFEYMQTRLPKYLMRHRPLMVGGVVTR"
     gene            complement(4591..5421)
                     /locus_tag="VF517_01095"
     CDS             complement(4591..5421)
                     /locus_tag="VF517_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX8051560.1"
                     /translation="MRVHAISTGTVRVPERQQRGVGPGPTRPILTLLDRHWTGPLPIN
                     VWAIEHPEGVIVVDTGETARAMEPGWFPRWHPYFRLAVRLSVRPEDEIGPQLRGLGIE
                     PGDVRRVVLTHMHTDHAGGLAHFPDSEILLSRDEHRVASGSLAKLRGFLPHRWPEWFS
                     PSLVDFQPEPFGPFGESLPITEAGDVRILPTPGHTAGHVSVVVDEGDQVLFLAGDTSY
                     NEGLLVDGALDGIAPDAKAARATNERIRELALQRPIVYLPTHDPGAADRLAARRRVGA
                     "
     gene            complement(5453..6058)
                     /locus_tag="VF517_01100"
     CDS             complement(5453..6058)
                     /locus_tag="VF517_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HEX8051561.1"
                     /translation="MDPERIRANLDRVRERIGAEVEICAAIKYVALDDLPALAEAGIA
                     LVGENRAQDMVAKHDAHGDLFEWDFIGALQSRKVRDVAPIARLVHSVASDSALRQLDK
                     HPAREVLLEVNVAGEVGKAGIAPAELGEYVGRCPVPVTGLMTMPPFAERPEDSRRHFA
                     RLAELAAEHGLSRLSMGTTQDYEIAVQEGATIVRLGTVLYA"
     gene            complement(6058..6786)
                     /locus_tag="VF517_01105"
     CDS             complement(6058..6786)
                     /locus_tag="VF517_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEX8051562.1"
                     /translation="MSGPRAFEVALPGARAAFSTRLGGVSAGPYESLNLGILTDDDQE
                     LVAENRRRVLAAAGVEAVAFGHQVHGAEVREWEGPPPAGHAAARDAAPGAALDRVDGH
                     ATETPGLGLLVLAADCLPLALATPERVAMLHCGWRGLAGGIVERALAGFAAAPAAALG
                     PCIGQCCYEVGPEVLGAFADLDGVADGRMLSLRAVAEAELRAGGVSAAEHVDLCTSCR
                     PDLFFSHRRDDGVTGRQCGIVVRA"
     gene            complement(6783..8027)
                     /locus_tag="VF517_01110"
     CDS             complement(6783..8027)
                     /locus_tag="VF517_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051563.1"
                     /translation="MPERLAERVRALPGMDALLPALQGLAPAYLVGGAVRDLIRGQRA
                     VDLDVAVEGDAPAVAREVAARTGGEAIVHERFGTATVRAPRVVADLATTRRERYEHPG
                     ALPDVESAPLEEDLARRDFAINAMAIALAGDRLGHLHDPHGGRADLDAGVVRVLHELS
                     FLDDPTRLLRAVRYEARLGGRMDRDTETLAREAIAGGALATVSGHRIRDELMDLLGEV
                     EVGRAVGRMADLELDRALDPALSADAEPVASAALACLETGADRALAALATLVSRDPDA
                     LAPWVETLGLRRSDRERVLAAARQAPSLVPPLRMELPRSAVHALLRCEPPETLALALA
                     LGAPAGPLLRFLADLRDVHLEIGGDDLIAAGVERSPAIGRALDEVLRRKLDGEVSGRE
                     EELEAALAAARAARGEPGGEAR"
     gene            complement(8028..8783)
                     /gene="hisF"
                     /locus_tag="VF517_01115"
     CDS             complement(8028..8783)
                     /gene="hisF"
                     /locus_tag="VF517_01115"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX8051564.1"
                     /translation="MLLKRVIPCLDVDKGRVVKGTNFVDIRDAGDPVELAIRYEAEGG
                     DELVFLDITASHEKRDTIVELARRTANDVFIPFTIGGGIRSVEDAQAVLDAGADKVSV
                     NSAAVARPELIGELAEVFGSQCVVLAIDAKRAGDGYGVFVNGGRKPVGRDAVEWAREG
                     TERGAGEILLTSMDRDGTEDGYELALTRAVSDAVEVPVIASGGAGNLDHLVDAVEEGH
                     ADAVLCASIFHYGSYTVREAKDRMRAAGIPVRE"
     gene            complement(8783..9508)
                     /gene="hisA"
                     /locus_tag="VF517_01120"
     CDS             complement(8783..9508)
                     /gene="hisA"
                     /locus_tag="VF517_01120"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX8051565.1"
                     /translation="MILLPAIDIRDGHAVRLERGDFARETVYVDDPLDAARAWAEAGA
                     RMLHVVDLDGAREGEPQNLDHLRRITSELTVPVQYGGGLRSLVAVREALAAGASRVVV
                     GTAAYNDLDFLDEIVATWGVRICVAIDVRGGNVSVSGWTQTTQMPARHVIERLQGRGV
                     KQFAYTNVDRDGMLEGPDLEDVRRVSEAIRGRFLYSGGIASVDDLRALRELRCVNLAG
                     VISGKALYEQRFTVAEGNEVLGD"
     gene            complement(9505..10107)
                     /gene="hisH"
                     /locus_tag="VF517_01125"
     CDS             complement(9505..10107)
                     /gene="hisH"
                     /locus_tag="VF517_01125"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX8051566.1"
                     /translation="MIAVVNYGMGNLRSVEKAFERVGATVEVTSAHDRIRAADHVVLP
                     GVGAFPKAMERIRAQGLDEVIAERVDAGVPLLGICLGMQLLFDSSTELGGAEGLGLVP
                     GAIDKLDSDGLKLPQIGWNEVRWRNGSPLREALPDRTAMYHVHTYAPQSVREEDVLGT
                     ATYGAEFVTAVQRPPVYGVQFHPEKSGPHGLALLGAFVQR"
     gene            complement(10104..10694)
                     /gene="hisB"
                     /locus_tag="VF517_01130"
     CDS             complement(10104..10694)
                     /gene="hisB"
                     /locus_tag="VF517_01130"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX8051567.1"
                     /translation="MSRSAEITRTTRETDIAMKLDLDGSGAGERATGVGFLDHMLDAV
                     ARHGRLDLDVRASGDIAETGPHHTVEDVGIVLGQALDEALGDRAGILRYGEAVVPMDE
                     SRASAAIDISGRPLAVFEGDWPQRPVADLEEGLAQEMLRAVASTAKLTLHVRVEAAGN
                     DHHVIEAAFKAFALALRRAVALDPTETGVPSTKGLL"
     gene            complement(10691..11944)
                     /gene="hisD"
                     /locus_tag="VF517_01135"
     CDS             complement(10691..11944)
                     /gene="hisD"
                     /locus_tag="VF517_01135"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX8051568.1"
                     /translation="MRVERLRNATAAEIRSGSPPPPEAEVRAILEQVRAEGDIAVREL
                     TERFDHAELAPEELAVPPAEIDNAVAALEPGVLAGLRAAIANVRAVARAQLRDPVRVD
                     LEAGHSVSISEQPVRRAAAYIPGGRAPYPSTVVMCAITAQVAGVDEIAVCAPPGPGGR
                     AHPTILAACGLCGVNEVYRMGGAQAIGALAYGTESVRPVDVIVGPGNRYVAEAKRQVA
                     GVVGIDGIAGPSELVIVMTDRVLPYPLALDLLAQGEHGPDSLLVAIAPDRGDLNALQK
                     CLEHHVPTSRSVLAERVVLVQTDDAAAAVRLADELAPEHLQLAGAKAEALVGRVRSAG
                     CVFVGVDGATAFGDYIAGSNHVLPTGGAARFQSALSPATFRRRMARVSLPFGTAARLA
                     PPAAALARAEGFPVHGDSMEERARQ"
     gene            complement(11941..12567)
                     /gene="hisG"
                     /locus_tag="VF517_01140"
     CDS             complement(11941..12567)
                     /gene="hisG"
                     /locus_tag="VF517_01140"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX8051569.1"
                     /translation="MSGLVIAIPRGALLGDALGLLDRLGIDTAEIRSNDRKLFFREAG
                     VITVRPSDVPTYVESGAADVGITGKDVLLEQAERDVYELLDLGFGGCRMIVATREGDD
                     PLGEPLRRRGLVRIATKYPQIARDHFAATGRQAEIVEVKGSVELAPLTGLADAIVDLT
                     ASGRTLVENGLVEREPILDSTARLIANPVAHRLKAAEMDGFVERIRAL"
     gene            complement(12564..13517)
                     /locus_tag="VF517_01145"
     CDS             complement(12564..13517)
                     /locus_tag="VF517_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX8051570.1"
                     /translation="MIHPVPPGTRDVLPDEMRELRTLTLRLSEAFERAGYGEVSTPSI
                     EYEDVLRRGDDRDAGAGFRLFDETGRILALRSDPTIPIARLVASRYDSVEGPLRFSYF
                     ENAYRAVASGSGHAREFLQGGVELIGVPGAEGDAEVIALVASALEEAGLRRHRIGVGD
                     ARLFRDRLAALDVGDEDRSELLRLLWRRDLPGLETRVERLGLPGDAARGLVALASLRG
                     GPEVLEGQQGTENLRELCALLEARGLADRVIFDLGMQRELGYYTGTIFEVYDPAVAFA
                     LGGGGRYDELLGRFGRPRPACGVGLDVTRVHVAQIEEERGS"
     gene            complement(13539..14837)
                     /gene="murA"
                     /locus_tag="VF517_01150"
     CDS             complement(13539..14837)
                     /gene="murA"
                     /locus_tag="VF517_01150"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX8051571.1"
                     /translation="MEKFVIEGGATLSGTVVPAGNKNAALPALAACLLTEEEVVLRNV
                     PRIRDVEAMVELLGSLGVTHEWREDNVVALCAAGVDHTDVDATAAERIRASFLLAGPL
                     LARFGRADMPPPGGDVIGRRRLDPHLDAFRALGVDVKFDRWYYLSAPRGLRPTDFFMD
                     EPSVMATENALMAAALTPGSTVIHNAASEPHVQDLSRLLLQMGARIEGIGSNVLIVHG
                     QDRLGGADYAIGPDYIEIGSFVALAAVTGGGLRVKDVVSDDLRMTKLGFERLGCHWDY
                     EGSDIVVPPDQELRIRDDEGDAISKIEDGPWPAFPADLTSIALALATQAEGMVLIHEK
                     MFENRLFFTDKLVSMGARVLLCDPHRAVVSGPSRLHGERMESPDIRAGMAMLIAALCA
                     EGRSEIGNIRQIDRGYERIDERLRALGANIRRVATEPAPV"
     gene            complement(14953..15633)
                     /locus_tag="VF517_01155"
     CDS             complement(14953..15633)
                     /locus_tag="VF517_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051572.1"
                     /translation="MIIKQGMTDVSPLARGIATLAAHPAPVATPPLPDPPSGVSRFLA
                     REGSVLTSAELAEAMRSGMLSAPVSDADFDKQVEGAVKLALSVLSKQPSGPVGIALFA
                     AQLAGVSLGIAAAHAQDPELLKFAERVQTGTRVAKGAIGGDLAPAAEVAMGALIDAVD
                     READVTSAARYLDKRPRELTAAVVDAAGVLAALPPEDPAKPPDPAKPPEDPALPPDPP
                     AEPPTAPA"
     gene            15695..16264
                     /locus_tag="VF517_01160"
     CDS             15695..16264
                     /locus_tag="VF517_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8051573.1"
                     /translation="MTPTRDDAWKLFCEWTESDSLRKHVLGVEAAMRAYAREYGEDEE
                     RWAVTGIVHDLDYERYPNLDTGHPRVALEELPKLGYPEDVVEAVAGHATFLNVPRRTL
                     MAKTLFAVDELSGFITACAYVRPTGIHGLTPKSVKKKLKQPSFAAAVNRDEVRQGAEE
                     LGVDFDEHITKVIAAMEERSAELGLDGQA"
     gene            complement(16255..16596)
                     /locus_tag="VF517_01165"
     CDS             complement(16255..16596)
                     /locus_tag="VF517_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051574.1"
                     /translation="MTLPLETTCTWCGNPVGPGDGFRLAELPGDRAAAFCRLEHVVPW
                     AIQGPHWEPGLPVEPPGVADSIDTCAHCGEALGDVRLILVRHRGEYRVPDGFCSVDHL
                     KDWAQSGGRYA"
     gene            16600..17694
                     /locus_tag="VF517_01170"
     CDS             16600..17694
                     /locus_tag="VF517_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX8051575.1"
                     /translation="MAVRIIVLEGDETGQELLDQAIRLLDPEVTGVAVELEHYDLSLE
                     NRRATQNQVVSDAGRALREARFGIKAATITPEGADDVGSPNRILREELDGKVIIRTGR
                     RIPGVTPVAGVHYPISVVRMAVGDAYGAKQWRETEDGDEVAYRTERISRDVCRAVAEH
                     SFRTARKIHGRVYGGPKWTVSPVYEGMLKEELDAASERHPEVAYQPVLIDATYAGLVS
                     GAADAPLVIPALNRDGDCLSDFVLPLFGSIAGAESVVLSLDEGFEVRAVMTEAPHGTA
                     PALLGKDIANPMAMIMAAAALLHYIGVDGHAEAELASRAVYEAVLEATATGVRTPDLG
                     GHATTTEFTSDVVARVRTKIDIWSSLGSTV"
     gene            complement(17684..18652)
                     /locus_tag="VF517_01175"
     CDS             complement(17684..18652)
                     /locus_tag="VF517_01175"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEX8051576.1"
                     /translation="MIHLRLVVPEDLAEAVNSLLCESPAVSSVIHLPGAAAKPLGDVL
                     LADVAREDASVIIGDLKELRLHELGTIAVEEVDSALSTAATEAEKAAAGLPSDAVVWE
                     EVESRTSEQTELGASFLAFMAIATMIAAIGVLLDSQILIIGAMVVGPEFGPIAGFCVA
                     VVQKRRDLARRSITALAIGFPLAITSAFLLTLILRATGSAPDELTNRPLTDFISHPDE
                     FSVIVALLAGVAGVVSLTAAKSGALIGVLISVTTIPAAGNIGVAAAYGDGHEWVGAME
                     QLSLNLVAILLAGIATLYFQRRLYVRRRREHLREPSRRLAGLPTRR"
     gene            complement(18649..20583)
                     /locus_tag="VF517_01180"
     CDS             complement(18649..20583)
                     /locus_tag="VF517_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX8051577.1"
                     /translation="MDVPRGRRDALGLTHARAFTTAVVLFVTIAAYTIITAAVDGALS
                     NERGLALGLVLLALATGLGALIGWRLDRRTIADRIGRTDELEKRVAEAEERAERESGR
                     AQRIETERDAEREWHREFRAQLIEAHHEGGVLGDASDIRALVLRVAVTLLESEKGLLL
                     SRTDEDGDGDLDLVAYQGFDNDPEHSAIAQRFAGRVLDRDETVREDAPRQSEDGERGS
                     EADSEIENLVAIPIYIRDRFSGVVVCANRDGGFEEWDDDVLIALGDHAGAVLDNSRLQ
                     GELRGAYLGTVRVLAEAIQAKDPFLRGHSEEVSGYVAAVADRLGVDSRRREELVFGSL
                     LHDVGKIGISERILLKPAALTPEERTLIELHPRIGYRLVEQVPALRPIAGAILHHLER
                     YDGDGYPAGLAGEAIPLEARIICVADSFSAMTAERPYRGRMSLEQACAELERCAGTQF
                     DPQVVEAFVEEVRRDPFSGDGSAAAAMEDPQVDAHRHGDEPVLGFGAHAIVDNLTLLY
                     SHRYFHEAVEAAAVRAADGAEPFSVVLAELTELAHLNRLEGYPAGDEAIQNAARAVQR
                     AAVLCGGTACRYSGRRLALIAPSTTGDEARRLAATVASELHGGARVAVGAAEWQPGDT
                     GFDVVARARLAMAPAAAPAG"
     gene            complement(20626..21036)
                     /locus_tag="VF517_01185"
     CDS             complement(20626..21036)
                     /locus_tag="VF517_01185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8051578.1"
                     /translation="MAQSFRHTIRVRFGECDPQGVVFNANWLAYFDVVMTELWRDRAG
                     DYAEMLAAGTDMVVAEATVRFLGAAGFDDNVDFDVTIARLGNTAVSTRIEASVEGRPV
                     ASGDMRHVFIDPATKAKKAMPDEVRAGLEPLLAD"
     gene            21098..21505
                     /locus_tag="VF517_01190"
     CDS             21098..21505
                     /locus_tag="VF517_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051579.1"
                     /translation="MFPLLRTRAARVLDVAIEFATLGEYRLEDPIAPVAPARGATRGT
                     EPAERLHGASVRPAARLVAPASAAAVRLRPAPAPAVPGPVRTRPKPASPLQDVGAIKQ
                     LALDFDGALPRPAVRTRGGAAPAAPQPCESPRR"
     gene            complement(21809..22501)
                     /locus_tag="VF517_01195"
     CDS             complement(21809..22501)
                     /locus_tag="VF517_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051580.1"
                     /translation="MTAPEAGEASGEPQAGAPAAEPSGAPRAGEAASGAPAGDSPSAT
                     PAGEAARLIPGFAPENELEERVTGDPELLAGLAWGKPREAHPEGAVGIHVAQLLDEID
                     SDRETGARRADLRFIALVHDSFKYRVRNWLPRQGRNHHATRARRFAERYTDDERLLAT
                     IEFHDRPYHLWKGMKKSSELDRGAFEEMLDRVPDHALFQRFIEVDGASEAKNPEPIRW
                     LRGELEQRGLLA"
     gene            complement(22498..22878)
                     /locus_tag="VF517_01200"
     CDS             complement(22498..22878)
                     /locus_tag="VF517_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051581.1"
                     /translation="MSVLAIVLIVIGVLLLVLFIGGLIRARREANRPEAAARIVAADR
                     ALEQARASDRGWDRAVLERTARTALDEQRPGSSWERIELELVDDRPGMAEDAAHLVAT
                     GSGGSARVVLARTEGGDWYAERVE"
     gene            complement(23310..23426)
                     /gene="rrf"
                     /locus_tag="VF517_01205"
     rRNA            complement(23310..23426)
                     /gene="rrf"
                     /locus_tag="VF517_01205"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(23518..>23935)
                     /locus_tag="VF517_01210"
     rRNA            complement(23518..>23935)
                     /locus_tag="VF517_01210"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3244 a         8912 c         8601 g         3178 t
ORIGIN      
        1 cttcggagac tggctcccgt gcagctgctt cgagcgcgcg aagcaggtcc acgggcagtt
       61 tggactcgca cgcgaggagt tcccctgttc ccggatgagt aaacccgagt agcgcgctgt
      121 gcaggaattg gcgcgttaga ccaagttcgg ccccatacgc cgaccccccg tagtcgggat
      181 ctccgcacac gggatggccg atctcggcga gatgcaccct gatctggtgg gtccggccgg
      241 tctcgagccg cacgcgcagg agcgtggtgt gagctagcgc gcgctcgatc gagaagtggg
      301 tgcgagccgc gcgcggggtg tcggtgcgcg gggagtggat cgtgcggttg cgccggtcgc
      361 ggccgatcgc gacgtcgatc gtgccgctgc gcgcgtcggg gcgcccggcg acgagcgcca
      421 ggtactcgcg acgcagctcg cggcgctgga gctgctcctg gagcgcgcgg agcacccggt
      481 cggacttcgc caccacgagc aggcccgagg tgtcgcggtc gagccggtgg acgatgccgg
      541 ggcggtacgg gtcgtctccg ccggcggcgc gccccgcgag cgcctcgacc agggtgccgg
      601 tcggatggcc cggagccggg tgcacgacca ccccggccgg cttgtccacc accagcaggt
      661 ggtcgtcctc gaacacgacc tcgtgcggag cggcatcggc gtcgccctcc ggcgcgatct
      721 cctcgggccg cgcctcgatc aggtcgccgg cctcgaccag gtggcgcttc tggcggcggg
      781 cgccgttcac ggtcacgcga tccgcctcga tcagccgctc ggcggccgag cgcgagccga
      841 cctccgggtg cgcgccgagc accacgtcga gccgggcgcc ggcctgttcg gcggcgaccg
      901 cgaagcggat cgggtcgggc gccgccgcgg tcatgcgccg gccgcgtccc cggcgcgcgc
      961 ttcgccggcg gcgtccccgg cgcgcggctc gccggccgcg tccccggcgc gcggctcgcc
     1021 ggccgcgccg cgcggcttgc gcggcccctc gaccacgtac agcagcccga ggatcccgat
     1081 cacgatcgcg gcgtcggcca ggttgaacgc cggccacagc gccggatcca ggtagtcgat
     1141 caccgacccg tcgcgcgcac ggtcggccag gttgcccagt gctccgcccg ccacggcccc
     1201 cacgggcagc cagagccagg gccggcgcgc gtgcagcgcg aagtagccga tcaggaacgc
     1261 cagcgccacc gccgtcagca ccacgatcag tgcgccgccg tcaccgagcg ccccgaacgc
     1321 gatgccctcg ttgcgcacgt acgtgaggtc gatcccgaag aagacgttgc gctcctcccc
     1381 gcgcgggatc ccgctcacca ccgcctgctt gaggacctgg tcgagcgcca cgacggcggc
     1441 cgcgaccacg cccgcgaggc gccactcgcg cacgggcgct agccccggat gagagccgcc
     1501 acgatgttgc cgaccacgat caggacgatc gtgcccacca tcgggctcag gtcgatcgcg
     1561 gccgggccca ggcgcgccat cgggaggatg cgccgccaca tgttcaggta cgggtcggtc
     1621 acgtcgcgga cgaacgtcag caccttgtcg agtgcgacgt tgtagggcat ccgcgtgatg
     1681 tagctcatca atacgcggac gaagatcagg atcacgtaga cgatgaccag cgtctcgatg
     1741 tagccggcga cgtcggcgcg gctcacgact tcgcccccga cgccgcggcg accgcctcgt
     1801 cgaacgccgc gcgcaccccg cccgcctcga gctccgcgag cccgcgggcg gtcgacccgc
     1861 ccggcgaggt cacgcgcgtg cgcaggccgc gcgtgtcgcc gccgctctcg cgcagcacgg
     1921 cggcggtgcc ggccatggtc tcgacggcca gccgcccggc cacgtccgtg gtcagcccct
     1981 gcttcacgcc cgcgtccacg agcgcctcga ccaccagcgc caggaacgcc ggcccgcagc
     2041 tcatcacggc catgccggcg tcgatcagct tctccggcag gtccacgacg gtgccgagcc
     2101 ggtcgagcag gccgcgcagc tcggactcgg gcccctgggc ggccagccgc ccgggcgcgt
     2161 agaggacgac accctggccg acctcgaccg ggatgctggg catcaggcgg tacaccggac
     2221 ggtcagggta cgcggcctcg acacgctcca gcgggacccc gccgaggatc gacacgaccg
     2281 cccgcgcccc cgcgccgatc tcgccggcca cctcctcgag ctgcgcgggc ttgtggcaga
     2341 gcacgaccgc gtcggcaccg gccgccaccg cggagttcga gtcgagcgcc tcgccgccga
     2401 cggcgctcgc cagcgcctcg gcgcgggagc ggtcgaggtc ggagaccagg accggctcgc
     2461 gcaggccgcg cacgatggcg ctcgccatgt tgcccgctcc gatgaagccg agtcgcatcg
     2521 ggactccgcc ggcccgctag gactggttga agaagccctt ctcgatcagc cgagcccgtt
     2581 cctcggcgga gacctcgacg ttgcgcggcg tcaggaggaa gaccttgtcg gcgatgcgct
     2641 gcattccacc gtcgagcgcg taggtgaggc ccgatgcgaa gtcgatcagg cgcttcgaga
     2701 gatcggtgtc ggtgccctgg aggttgagga tcacgggcac gtcgtccttg aactggtcgg
     2761 cgatctgctg cgcgtcgttg aagctcttcg gcacgaccag gtggacctgc acggactcgg
     2821 cgcggccgtt gctcaccggc cgcaggacgc gcgtctcgcg cgcgctgcgc gggggctcgt
     2881 cggcgaagat gtcgtcgaag tcgtcgcgcc ggcggcggct ctggagacgg cgcacgttcg
     2941 ggcgctcgcg gtagcggtcc tcgatctcct cctcgggctg cggctcggag tcgtgatcgc
     3001 gatagcgatc ctcctcctcc tcgaggccga aatagaccag cgtcttgtgc caggtgtccc
     3061 ggagtgccat gtcgcggggt tcttcgcgtg agcgggcgaa aaccctttcg tttacaagtt
     3121 atcgggtgcc cccgcgcctc gccgccgaag aatccgggtc cggcccgccc gtggtgctgc
     3181 tgcacggcct gtcggccacc cggcgctacg tggtccaccg gtcgaaggcg ctcccccgcg
     3241 cggggttccg cacgatcgcc tacgacgcgc gcgggcacgg cgagtcggag gctccggacg
     3301 accccgacgc ctacgactac gccgacctct gcgcagacct cgccgcgctg atggacgagc
     3361 acggcctcga ccgcgcggcg ctcgccggcg tgtcgatggg cgcccacacg gcgctgcgat
     3421 tcgcgctcga gcaccccgag cgggtgagcg cggtcgtggc gatcacgccc ggcttcaccg
     3481 gcgaggcggc aaccgacacg gggccgtggg accgaatggc ggacggcctc gagcgcggcg
     3541 gcgtcgaggg cttcgtgtcc gaatggggcg cccgcgacgt ggccgagcgc taccgcgagg
     3601 ccgcgctcga gcacgcgcgc cagcggctct cccagcaccg cgacctcgcg gcggttgccc
     3661 gcgcgctacg ggtggtgccg cgctcggagc cgttccccgg gctgagctcg ctccgagcgg
     3721 tcgaggcccc ggcgctcgtg gtcggctcac gcgacggcgc ggaccccatg cacccgctcg
     3781 cggtggccga ggcctacgcc ggggagctgc cggacgcccg cctgctcgtc gaggacgagg
     3841 gcaggccgcc gctggcgtgg cagggggcgc ggctgtcgaa ggcgatcgcg cagttcctcg
     3901 gaggcgcggg gtccggcggg gccggcgccg gggccggcgg cgccgcgggc tagcgggtca
     3961 cgaccccgcc gaccatcagg ggacggtgcc tcatgaggta cttcggcagc cgcgtctgca
     4021 tgtactcgaa ggtcctgacc gtgcccacgc cgtcgagcat ccgcgcccag tcgtcgccct
     4081 cgagcaggcg ctcgtccccg aagtcctggc ggccgtacca gcgcgacggg acgctgaaga
     4141 cgaagacggg cgccgcggcg agcgcctcct gtgccagccg ccggacgtcc tcgtcggagt
     4201 agtgctccag cacgccctgg gagaagacca cgtcgaagct ctcgtccagc tccgagagcg
     4261 cgaagatgtc gtgctggagg agccgcagct gggtgttcca gcgctctgcg ttctcacccg
     4321 cacgctcgag caccgtcgcg tcgaggtcgc aggccaccgc ctcgatgccc gccatcgcga
     4381 ggaacacgct catggtcgcc ggccccgacc cggcctcgag cgtcttgctc ggcttcgcct
     4441 cgaatatctc ggcgaggaac tcgcggtggt agtgcaggtt gctgaggaag aactcgaagc
     4501 cctgcggctt ttcgtagaac cgcgcccaac cgccgtcctg gccgtgtctc tgcgcgcctg
     4561 cctccacgga cgccgaccgt accaggcgct ttaggccccg acccggcgtc gtgcggccag
     4621 ccgatcggcg gcgcccgggt cgtgcgtggg caggtagacg atcgggcgct ggagggccag
     4681 ctcgcggatg cgctcgttcg tggcgcgggc ggccttcgcg tcgggcgcga tgccgtcgag
     4741 cgcgccgtcc accagcagtc cctcgttgta cgacgtgtcg ccggccagga acagcacctg
     4801 atcgccctcg tccacgacga ccgacacgtg accggcggtg tgcccggggg tcgggaggat
     4861 ccgcacgtcg ccggcctcgg tgatcggcag cgactccccg aacggcccga acggctccgg
     4921 ctggaagtcg acgagcgacg gggagaacca ctccggccag cggtgcggga ggaagcccct
     4981 cagcttggcc agcgacccgg aggcgacccg gtgctcgtcg cgcgagagga ggatctcgct
     5041 gtcggggaag tgagcgagcc cgccggcgtg gtcggtgtgc atgtgcgtga ggaccaccct
     5101 gcgtacgtcg cccggctcga tgccgagacc gcgcagctgc ggcccgatct cgtcctccgg
     5161 gcgcaccgag agccgcacgg cgaggcggaa gtacggatgc cagcgcggga accagccggg
     5221 ctccatcgcg cgcgcggtct cgccggtgtc gaccacgatc acgccctcgg ggtgctcgat
     5281 cgcccagacg ttgatcggca gcggaccggt ccagtggcgg tcgagcagcg tcaggatcgg
     5341 acgcgtgggc ccggggccca cgccgcgctg ctggcgctcg ggcacccgga cggtgccggt
     5401 gctgatcgcg tggacgcgca cggtcaggcg ccgatcaggg gtggctcggc catcaggcgt
     5461 agagcaccgt accgaggcgc acgatcgtgg cgccctcctg cacggcgatc tcgtagtcct
     5521 gcgtggtgcc catcgagagc cggctcagtc cgtgctcggc cgccagctcg gcaagccgcg
     5581 cgaagtgccg gcggctgtcc tccgggcgct cggcgaacgg cggcatcgtc atcaggccgg
     5641 tcaccggcac cgggcagcgc ccgacgtact cacccagctc ggccggagcg atcccggcct
     5701 tgcccacctc ccccgccacg ttcacctcga gcagcacctc gcgcgcgggg tgcttgtcga
     5761 gctgcctcag cgcggagtcc gaggcgaccg agtggacgag gcgcgcgatc ggggcgacgt
     5821 cgcgcacctt gcgtgactgg agcgcgccga tgaagtccca ctcgaacagg tcgccgtgcg
     5881 cgtcgtgctt ggcgaccatg tcctgcgccc ggttctcgcc gaccagcgcg atgccggcct
     5941 cggcgagcgc cggcaggtcg tcgagcgcga cgtacttgat cgcggcgcag atctcgacct
     6001 cggcgccgat ccgctcgcgc acgcggtcga ggttcgcgcg gatgcgctcc gggtccatca
     6061 cgcgcgcacc acgatgccgc actggcggcc ggtcacgccg tcgtcgcggc ggtgggagaa
     6121 gaacaggtcc gggcggcagc tcgtgcagag gtccacgtgc tcggccgcgc tcacgccgcc
     6181 cgcgcgcagc tccgcctcgg ccaccgcgcg caggctcagc atgcgcccgt cggccacccc
     6241 gtcgaggtcg gcgaaggcgc cgagcacctc gggtccgacc tcgtagcagc actgcccgat
     6301 gcagggaccg agcgcggcgg ccggcgctgc ggcaaagccc gccagcgcgc gctcgacgat
     6361 gccgcccgcg agcccgcgcc aaccgcagtg cagcatcgcc acgcgctccg gcgtggcgag
     6421 cgcgagcggc aggcagtcgg ccgcgagcac gagcaggccg agcccgggag tctcggtggc
     6481 gtgcccgtcc acccggtcga gcgccgcccc cggggccgcg tcgcgcgcgg ccgcgtggcc
     6541 ggcgggcggg ggcccctccc actcgcgcac ctccgcgccg tgcacctggt gaccgaacgc
     6601 gaccgcctcg acgcccgcgg ccgcgagcac gcgccggcgg ttctcggcca ccagctcctg
     6661 gtcgtcgtcg gtgaggatcc cgaggttgag tgactcgtag ggaccggcgc tcacgccgcc
     6721 gagccgcgtc gagaacgccg cccgggcgcc cggcagggcg acctcgaagg cgcgcgggcc
     6781 gctcaccgcg cctctccgcc gggctccccg cgagccgcgc gcgcggcggc gagcgccgcc
     6841 tccagctcct cctcgcgccc cgagacctcc ccgtcgagct tgcgccgcag cacctcgtcg
     6901 agcgcgcggc cgatcgccgg ggagcgctcc acgccggcgg cgatcaggtc gtcgccgccg
     6961 atctcgaggt gcacgtcgcg caggtcggcg aggaagcgca gcagcggccc ggcgggcgcg
     7021 ccgagcgcga gcgccagcgc cagcgtctcc ggaggctcgc agcgcagcag cgcgtgcacc
     7081 gccgagcgcg gcagctccat ccgcagcggc ggcacgagcg acggcgcctg gcgggccgcg
     7141 gcgagcacgc gctccctgtc gctgcggcgc agcccgagcg tctcgaccca cggcgcgagc
     7201 gcgtcgggat cgcgcgacac cagcgtcgcc agcgcggcaa gtgcgcggtc ggcgcccgtc
     7261 tcgaggcacg cgagcgcggc cgaggccacc ggctccgcgt cggccgacag cgccggatcg
     7321 agcgcgcggt cgagctccag gtccgccatc cgccccaccg cccgccccac ctcgacctcg
     7381 ccgagcaggt ccatcagctc gtcgcgaatc cggtgtcccg agaccgtcgc gagcgccccg
     7441 cccgcgatcg cctcacgcgc gagcgtctcc gtgtcgcggt ccatgcgccc gccgaggcgc
     7501 gcctcgtagc gcaccgcgcg cagcaggcgc gtcgggtcgt cgaggaagct gagctcgtgc
     7561 agcacgcgca ccacgcccgc gtcgagatcc gcgcgcccgc cgtgcgggtc gtgcaggtgg
     7621 ccgagccggt cgcccgcgag cgcgatcgcc atcgcgttga tcgcgaagtc gcgccgggcg
     7681 aggtcctcct cgagcggcgc ggactccacg tcgggcagag cacccgggtg ctcgtagcgc
     7741 tcgcggcgcg tcgtggcgag gtcggcgacc acccgcggcg ccctcacggt cgccgtgccg
     7801 aagcgctcgt gcacgatcgc ctcgccgccg gtccgcgccg ccacctcgcg cgcaacggcg
     7861 ggcgcgtccc cctccaccgc cacgtcgagg tcgacggccc tctgcccgcg gatcaggtcg
     7921 cgcaccgcgc cgcccactag gtacgccgga gcgagaccct ggagcgccgg cagcagggcg
     7981 tccatccccg gcagcgcgcg cacccgctcg gccagccgct cgggcatcta ctccctcacg
     8041 ggaatcccgg ccgcccgcat gcggtccttg gcctcgcgca cggtgtagga cccgtagtgg
     8101 aagatcgacg cgcacagcac ggcgtcggcg tgcccctcct cgacggcgtc gacgaggtga
     8161 tcgaggttcc cggcgccgcc cgacgcgatc acgggcacct cgaccgcgtc cgagacggcg
     8221 cgcgtgagcg cgagctcgta gccgtcctcg gtgccgtcgc ggtccatgct cgtgaggagg
     8281 atctcccccg cgccccgctc ggtgccctcg cgcgcccact ccacggcgtc gcgcccgacc
     8341 ggcttgcgcc cgccgttcac gaacaccccg taaccgtcgc cggcgcgctt cgcgtcgatc
     8401 gcgagcacca cgcactgcga gccgaacacc tcggccagct cgccgatcag ctccggccgc
     8461 gccaccgcgg cgctgttcac cgagaccttg tccgcgcccg cgtcgagcac ggcctgcgcg
     8521 tcctcgaccg agcggatccc tccgccgatg gtgaacggga tgaagacgtc gttcgccgtg
     8581 cggcgggcca gctcgacgat cgtgtcgcgc ttctcgtgcg aggccgtgat gtcgaggaag
     8641 acgagctcgt cgccgccctc ggcctcgtag cgaatcgcca gctcgaccgg gtcgccggcg
     8701 tcgcggatgt ccacgaagtt cgtgcccttc acgacccgcc ccttgtctac gtcgaggcac
     8761 gggatcacgc gcttcagcag catcagtcgc cgagcacctc gttgccctcg gccacggtga
     8821 agcgctgctc gtagagcgcc ttgccggaga tcacgcccgc caggttcacg caacgcagct
     8881 cgcgcagcgc gcgcaggtcg tcgaccgacg cgatcccgcc cgagtagagg aaccgcccgc
     8941 ggatcgcctc cgacacgcgc cgcacgtcct cgaggtccgg cccctcgagc atcccgtcgc
     9001 ggtccacgtt cgtgtaggcg aactgcttga cgccgcggcc ctgcagccgc tcgatcacgt
     9061 ggcgtgcggg catctgcgtg gtctgggtcc agccggatac cgacacgttg ccgccgcgga
     9121 cgtcgatcgc cacgcagatg cgcacacccc aggtggccac gatctcgtcg aggaagtcga
     9181 ggtcgttgta cgccgcggtg cccaccacga cgcgcgacgc ccccgccgcc agcgcctcgc
     9241 gcaccgcgac cagggagcgc aggccgccgc cgtactgcac cggcacggtc agctcggacg
     9301 tgatccggcg taggtggtcg aggttctgcg gctcgccctc gcgcgcgccg tcgagatcca
     9361 ccacgtgcag catccgcgcg cccgcctccg cccaggcgcg cgccgcgtcg agcgggtcgt
     9421 ccacgtacac ggtctcgcgc gcgaagtcgc cgcgctcgag ccgcacggcg tggccgtcgc
     9481 ggatgtcgat cgccgggagg aggatcaccg ctgcacgaag gcgccgagca gggcgagccc
     9541 gtgcgggccc gacttctcgg ggtggaactg gacgccgtac accggcggcc gctgcacggc
     9601 cgtgacgaac tcggcgccgt acgtggcggt gccaagaacg tcctcctcgc gcacggactg
     9661 cggggcgtag gtgtgcacgt ggtacatcgc cgtgcggtcc ggaagcgcct cgcggagcgg
     9721 cgatccgttc ctccaccgca cctcgttcca gccgatctgc ggaagcttga gcccgtccga
     9781 gtcgagcttg tcgatcgcgc cgggcaccag cccgaggccc tccgcaccgc ccagctccgt
     9841 cgaggagtcg aacaggagct gcatgccgag gcagatcccg agcagcggca cgcccgcgtc
     9901 cacgcgctcg gcgatcacct cgtcgagtcc ctgcgcgcgg atgcgctcca tcgccttcgg
     9961 gaacgcgccc acccccggca gcacgacgtg gtcggcggcc cggatgcggt cgtgcgcgct
    10021 ggtcacctcc acggtcgcgc ccacgcgctc gaatgccttc tccaccgagc ggaggttgcc
    10081 catcccgtag ttcacgacgg cgatcacagg agccccttgg tggacggcac gcccgtctcg
    10141 gtgggatcga gcgccacggc gcgccgcagg gccagcgcga acgccttgaa cgcggcctcg
    10201 atcacgtggt ggtcgttgcc cgccgcctcg acgcgcacgt gcagcgtgag ctttgccgtc
    10261 gaggcgaccg cgcgcagcat ctcctgggcg agcccctcct cgaggtcggc caccggccgc
    10321 tgcggccagt cgccctcgaa caccgccagc ggccggcccg agatgtcgat cgccgcgctc
    10381 gcgcgcgact cgtccatcgg aacgacggcc tcgccgtagc gcaggatccc cgcgcggtcg
    10441 ccgagcgcct cgtcgagcgc ctgaccgagc acgatgccga cgtcctcgac cgtgtggtgc
    10501 ggccccgtct cggcgatgtc gccgctcgcg cgcacgtcga ggtcgagccg gccatggcgc
    10561 gccacggcgt cgagcatgtg gtcgaggaag ccgaccccgg tggcgcgctc gccggcgcca
    10621 gacccgtcga ggtcgagctt catggcgata tccgtctcgc gggtcgtgcg ggtgatctcg
    10681 gcgctgcggc tcattggcgg gcgcgctcct ccatcgagtc cccgtgcacc gggaagccct
    10741 cggctcgggc cagcgcggcg gcgggcggag cgaggcgcgc ggccgtaccg aagggcaacg
    10801 atacccgcgc catccggcgc cggaaggtgg ccggcgagag cgcgctctgg aagcgcgcgg
    10861 cgccgcccgt gggcagcacg tggttggacc ccgcgatgta gtcgccgaac gccgtcgcgc
    10921 cgtcgacccc gacgaacacg cagccggccg agcgcaccct tccgacgagt gcctcggcct
    10981 tcgccccggc cagctggagg tgctcgggcg cgagctcgtc ggcgagccgg acggccgccg
    11041 cggcgtcgtc cgtctgcacg agcacgacgc gctccgccag cacgctccgg gatgtcggca
    11101 cgtggtgctc gagacacttc tggagcgcat tgagatcgcc gcggtccggc gcgatcgcca
    11161 cgagcaggga gtccggcccg tgctccccct gcgcgagcag gtcgagtgcg agcgggtagg
    11221 gcagcacgcg gtcggtcatc acgatcacga gctcgctggg gccggcgatt ccgtcgatgc
    11281 cgaccacccc cgccacctgc cgcttcgcct ccgccacgta gcggttgccc ggtccgacga
    11341 tcacgtccac cggcctgacc gactccgtcc cgtacgcgag cgcgccgatc gcctgcgcac
    11401 cgcccatccg gtagacctcg ttcacgccgc agaggccgca cgcggcgagg atcgtcgggt
    11461 gcgcgcggcc gccgggtccg ggtggggcgc agaccgcgat ctcgtccacg ccggccacct
    11521 gcgcggttat cgcgcacatc acgacggtcg acgggtaggg cgctcgtccg cccgggatgt
    11581 aggccgcggc acggcgcacg ggctgctctg agatcgagac ggagtggccg gcctccaggt
    11641 ccacgcgcac ggggtcgcgg agctgcgcac gggccacggc gcgaacgttc gcgatcgccg
    11701 cccgcagccc cgcgagcacg cccggctcga gcgcggccac cgcgttgtcg atctcggccg
    11761 gcggcaccgc cagctcctcg ggcgcgagct cggcgtggtc gaagcgctcg gtgagctcgc
    11821 gaaccgcgat gtccccctcg gcccgtacct gctcgaggat cgcgcgcacc tcggcctccg
    11881 gcgggggcgg agagcccgac cggatctccg ccgccgtcgc gttgcgcagg cgctcgaccc
    11941 tcacagcgcc cggatcctct ccacgaaccc gtccatctcg gcggccttca gcctgtgcgc
    12001 gaccgggttg gcgatcaggc gcgcggtcga gtcgaggatc ggctcgcgct cgaccagccc
    12061 gttctcgacg agggtgcggc cgctggcggt gaggtcgacg atcgcgtcgg ccaggccggt
    12121 cagcggcgcc agctcgacgg accccttcac ctccacgatc tcggcctggc ggccggtggc
    12181 cgcgaagtgg tcgcgggcga tctgcgggta cttggtcgcg atacgcacga gcccgcggcg
    12241 gcgcagcggc tccccgagcg ggtcgtcgcc ctcgcgcgtg gccacgatca tccggcagcc
    12301 gccgaagccg aggtcgagca gctcgtagac gtcgcgctcg gcctgctcga gcagcacgtc
    12361 cttgccggtg atccccacgt cggccgcgcc cgactcgacg taggtgggca cgtcgctcgg
    12421 ccgcacggtg atcacgccgg cctcgcggaa gaacagcttg cggtcgttcg agcggatctc
    12481 cgcggtgtcg atgccgaggc ggtcgagcag gccgagggcg tcgccgagca gcgctccgcg
    12541 cgggatcgcg atcacgaggc cgctcacgac ccgcgctcct cctcgatctg cgcgacgtgc
    12601 acgcgcgtca cgtcgagccc gacgccgcac gcggggcgcg ggcgcccgaa gcggccgagc
    12661 agctcgtcgt agcgaccgcc gccgccgagc gcgaacgcca cggccgggtc gtagacctcg
    12721 aagatcgtgc ccgtgtagta ccccagctcg cgctgcatcc cgaggtcgaa gatcacgcgg
    12781 tcggccaggc cgcgggcctc gagcagcgcg cagagctcgc gcaggttctc ggtgccctgc
    12841 tggccctcga ggacctccgg cccgccgcgc aggctcgcga gcgccacgag accgcgggcg
    12901 gcgtcgcccg gcaagccgag ccgctcgacg cgggtctcga gcccgggcag gtcgcgccgc
    12961 cacagcagcc gcagcagctc gctcctgtcc tcgtcgccga cgtcgagggc cgccagccgg
    13021 tcgcggaaca ggcgggcgtc gcccaccccg atccggtgcc ggcgcagccc cgcctcctcc
    13081 agcgcagatg ccacgagcgc gatcacctcg gcgtcgccct ccgcgcccgg cacgccgatc
    13141 agctccaccc caccctggag gaactcgcgc gcgtgcccgc tcccgctcgc cactgcgcgg
    13201 tacgcgttct cgaagtacga gaagcgcagc ggcccctcga cggagtcgta gcggctggcc
    13261 acgagccgcg cgatcgggat cgtcgggtcg ctgcgcagcg cgaggattcg gccggtctcg
    13321 tcgaagagcc tgaagcccgc gccggcgtcc cggtcgtcgc cccggcgcag cacgtcctcg
    13381 tactcgatcg acggcgtcga tacctcgccg tagcccgcgc gctcgaacgc ctcggacagg
    13441 cgcagcgtga gcgtgcgcag ctcgcgcatc tcgtcgggca gcacgtcgcg cgtgccgggt
    13501 ggaaccgggt ggatcacaga ggcctccggc gccggaggct agaccggagc cggctcggta
    13561 gcgacgcggc ggatgttcgc gccgagcgct cggagccgct cgtcgatgcg ctcgtagccg
    13621 cggtcgatct ggcggatgtt gccgatctcg gacctgccct cggcgcacag cgctgcgatg
    13681 agcatcgcca tgccggcccg gatgtcaggg ctctccatgc gctcgccgtg aagccggctc
    13741 gggccgctta ccaccgcgcg atggggatcg cacagcagga cccgagcgcc catggatacg
    13801 agcttgtctg tgaagaaaag gcggttctcg aacatcttct cgtggatcaa gaccataccc
    13861 tcggcctgcg tggccagtgc cagcgcgatg ctggtgaggt ccgccgggaa ggccggccag
    13921 ggcccgtcct cgatcttcga gatggcgtca ccctcgtcgt cgcggatgcg cagctcctgg
    13981 tccggcggga ccacgatgtc gctgccctcg tagtcccagt ggcagccgag tcgctcgaat
    14041 ccgagcttgg tcatccgcag gtcatcgctc acgacgtcct tgacccgcag cccgccgccc
    14101 gtcacggccg cgagtgccac gaagctgccg atctcgatgt agtcgggccc gatcgcgtag
    14161 tcggcgccgc cgaggcggtc ctgaccgtgg acgatcagga cgttcgagcc gatgccctcg
    14221 atccgcgcgc ccatctggag cagcagccgc gagaggtcct gaacgtgcgg ctcggaggcc
    14281 gcgttgtgga tcacggtcga gccaggcgtc agcgccgcgg ccatcagcgc gttctcggtg
    14341 gccatcaccg acggctcgtc catgaagaag tcggtcgggc gcaggccgcg cggcgcgctc
    14401 aggtagtacc agcggtcgaa cttcacgtcg acgcccagcg cgcggaaggc atcgaggtgc
    14461 ggatcgagcc ggcggcggcc gatcacgtcg ccgccgggcg ggggcatgtc ggcgcggccg
    14521 aagcgcgcca gcagcgggcc ggccagcagg aacgaggcgc ggatccgctc ggcggcggtg
    14581 gcgtcgacgt cggtgtggtc gacccctgcg gcgcacagcg cgacgacgtt gtcctcgcgc
    14641 cactcgtgcg tcacgccgag gctgccgagc agctcgacca tcgcctccac gtcgcggatg
    14701 cgcgggacgt tgcgcagcac gacctcctcc tcggtgagga ggcaggcggc gagtgcgggc
    14761 agcgcggcgt tcttgttccc ggcgggcaca accgtgccgg agagcgtcgc gcccccttcg
    14821 atgacgaatt tctccatgcg agcggccgct gacgaggggt ctgcgagcgg cgccgagagc
    14881 gtaccgaagg ccatcgcggc ctcattcgac ggaggcgggc gcggccctcc cgcccgagcg
    14941 cggccctgcg gctcaggcgg gggcggtcgg aggctccgcg ggcgggtccg gcggcagcgc
    15001 ggggtcctcc ggcggcttcg cggggtccgg cggcttcgcg gggtcctccg gcggcagcgc
    15061 ggcgagcacc cccgcggcgt ccaccaccgc cgcggtcagc tcgcgcgggc gcttgtccag
    15121 gtatcgcgcg gcgctcgtca cgtcggcttc gcggtcgacc gcatcgatga gcgcccccat
    15181 cgcgacctcg gccgcgggtg cgagatcccc gccgatcgcc cccttcgcga ccctcgtgcc
    15241 cgtctggacg cgctccgcga acttcaggag ctccgggtcc tgcgcgtgcg ccgcggcgat
    15301 gccgagcgat acgccggcga gctgcgcggc gaacagcgcg atcccgaccg gcccgctcgg
    15361 ctgcttgctc agcaccgaga gcgcgagctt gaccgccccc tccacctgct tgtcgaagtc
    15421 cgcgtcggac acgggcgccg agagcatccc gctgcgcatc gcctcggcga gctcggcgct
    15481 ggtgaggacc gagccctcgc gtgcgaggaa ccggctcacg ccggacggcg ggtcgggtaa
    15541 cggcggggtc gcgaccggcg cgggatgggc cgcgagcgtc gcgatccccc gcgcgagcgg
    15601 gctcacgtcc gtcatgccct gcttgatgat catctggcgc ccacgcgctc atcgtaaacc
    15661 cgcgcgcgcc gatctcgcag tagctttggc cgccgtgacg ccgacccgcg acgacgcctg
    15721 gaagctcttc tgcgagtgga ccgaatcgga ctcgctgcgc aagcacgtgc tcggcgtgga
    15781 ggcggcgatg cgcgcctacg cccgcgagta cggcgaggac gaggagcgct gggcggtcac
    15841 cggaatcgtc cacgacctcg actacgagcg ctacccgaac ctcgacaccg ggcacccgcg
    15901 cgtcgccctc gaggagctgc cgaagctcgg ctatccggag gatgtggtcg aggcggtcgc
    15961 cggccacgcc acgttcctga acgtcccgcg ccgcacgctc atggcgaaga cgctgttcgc
    16021 ggtggacgag ctgtcgggct tcatcacggc gtgcgcgtac gtcaggccga ccggcatcca
    16081 cgggctgacc cccaagtccg tgaagaagaa gctcaagcag cccagctttg ccgcggcggt
    16141 gaaccgcgac gaggtgcgcc agggcgcgga ggaattgggc gtcgacttcg acgagcacat
    16201 cacgaaggtg atcgctgcga tggaggagcg ctcagcggag ctcgggctcg acggtcaggc
    16261 gtaacggccg ccggactgag cccagtcctt caggtggtcg acggagcaga agccgtcggg
    16321 gacgcggtac tcgccgcggt gtcgcacgag gatcagccgc acgtcgccga gggcctcgcc
    16381 gcagtgcgca cacgtgtcga tcgagtcggc gaccccgggc ggctcgaccg gcagcccggg
    16441 ctcccagtgc gggccctgga tcgcccacgg caccacgtgc tcgagccggc agaacgccgc
    16501 ggcgcggtcg ccgggcagct cggcgaggcg gaacccgtcg ccgggcccga ccgggttccc
    16561 gcaccaggtg cacgtggttt ccagaggtag agtcacggcg tggccgtgcg gattatcgtc
    16621 ctcgagggcg acgagacggg gcaggagctg ctcgaccagg cgatccgcct cctcgacccc
    16681 gaggtgacag gggtcgccgt cgagctcgag cactacgacc tctcgctgga gaaccgccgc
    16741 gccacgcaga accaggtggt gagcgacgcc gggcgggcgc tgcgcgaggc acgcttcggc
    16801 atcaaggccg cgacgatcac gcccgagggc gccgacgacg tcggcagccc caaccgcatc
    16861 ctccgcgagg agctcgacgg caaggtgatc atccgcaccg gccgccggat ccccggcgtg
    16921 acgccggtcg ccggcgtcca ctacccgatc tcggtggtgc gcatggccgt cggcgacgcc
    16981 tacggcgcga agcagtggcg cgagaccgag gacggcgacg aggtcgccta ccgcaccgag
    17041 cggatctcgc gcgacgtctg ccgcgccgtg gccgagcaca gcttccgcac cgcgaggaag
    17101 atccacgggc gcgtgtacgg cgggccgaag tggacggtct cgccggtcta cgaggggatg
    17161 ctgaaggagg agctcgacgc cgcgtccgag cgccacccgg aggtggccta ccagcccgtc
    17221 ctgatcgacg cgacctacgc ggggctcgtc tccggggcgg ccgacgcgcc gctggtgatc
    17281 ccggcgctca accgcgacgg cgactgcctg tccgacttcg tgctgccgct gttcggctcg
    17341 atcgcgggcg ccgagtcggt cgtgctctcg ctcgacgagg ggttcgaggt gcgcgccgtg
    17401 atgacagagg cgccgcacgg caccgccccc gcgctgctcg gcaaggacat cgcgaacccg
    17461 atggcgatga tcatggccgc cgcggcgctg ctccactaca tcggcgtcga cgggcacgcc
    17521 gaggcggagc tggcctcgcg cgcggtctac gaggccgtgc tcgaggcgac ggccacgggc
    17581 gtgcgcacgc cggacctcgg cggccacgcg accacgacgg agttcacctc agacgtcgtc
    17641 gcgcgcgtgc gcacgaagat cgacatctgg tcctcgctcg ggtctaccgt ctagtcggaa
    17701 gccccgcgag ccgccgtgac ggttcgcgca ggtgctcgcg ccggcggcgt acgtagaggc
    17761 ggcgctggaa gtagagggtc gcgatgcccg ccagcaggat cgccaccagg ttcagcgaga
    17821 gctgctccat cgcccccacc cactcgtgtc cgtccccgta ggccgccgcc acgccgatgt
    17881 tccccgcggc ggggatcgtc gtgaccgaga tcagcacgcc gatcagcgcg cccgacttcg
    17941 ccgcggtgag cgacaccacg ccggcgactc cggccagcag cgccacgatc accgagaact
    18001 cgtcggggtg cgagatgaag tccgtgagcg gccggttggt caactcgtcg ggcgccgacc
    18061 cggtggcgcg gaggatcagc gtgagcagaa aggcgctggt gatcgccagg gggaagccga
    18121 tcgcgagcgc ggtgatcgag cgccgcgcca ggtccctgcg cttctgcacg acggcgacgc
    18181 agaaccccgc gatcgggccg aactccggcc ccacgaccat cgcgccgatg atcaggatct
    18241 gcgagtcgag caggacgccg atcgcggcga tcatcgtcgc gatcgccatg aacgcgagga
    18301 agctggcgcc cagctcggtc tgctccgagg tgcgcgactc gacctcctcc cagaccacgg
    18361 cgtcggacgg caggcccgcg gcggccttct ccgcctcggt tgcggcggtg gagagcgcgc
    18421 tgtccacctc ctcgacggcg atcgtcccga gctcgtggag cctcagctcc ttcagatcgc
    18481 cgatgatcac gctggcgtcc tcgcgcgcca cgtcggccag cagcacgtcg ccgagcggct
    18541 tcgcggcggc gccggggagg tggatcacgc tcgagaccgc gggcgactcg cagagcaggc
    18601 tgttgacggc ttccgcgagg tcctccggca ctacgagccg gaggtggatc atccggccgg
    18661 agcggccgcg ggcgccatgg cgaggcgcgc tcgcgcgacc acgtcgaagc ccgtgtcccc
    18721 cggctgccac tcggcggcgc ccacggccac ccgagcgccg ccgtgcagct cggaggcgac
    18781 ggtcgcggcg agccgccgcg cctcgtcccc ggtcgtgctc ggcgcgatca gcgcgaggcg
    18841 ccggccgctg tagcggcagg ccgtgccgcc gcagaggacg gcggcgcgct ggacggcgcg
    18901 cgcggcgttc tggatcgcct cgtcgcccgc ggggtagccc tcgagccggt tgaggtgggc
    18961 cagctcggtg agctcggcca gcaccaccga aaatggctcc gcgccgtcgg ctgcccgcac
    19021 ggcggcggcc tcgacggcct cgtggaagta gcggtggctg tagagcagcg tgaggttgtc
    19081 cacgatcgcg tgcgcgccga agccgagcac cggctcgtcg ccgtggcgat gcgcgtccac
    19141 ctgggggtcc tccatcgcgg cggcggcgct gccgtcgccc gaaaacgggt cgcggcggac
    19201 ctcctccacg aacgcctcga ccacctgcgg gtcgaactgc gtgccggcgc agcgctcgag
    19261 ctcggcgcag gcctgctcga gcgacatgcg gcctcggtag gggcgctcgg cggtcatcgc
    19321 gctgaacgag tcggccacgc agatgatgcg tgcctcgagg gggatcgcct cgccggcgag
    19381 gccggccggg tagccgtcgc cgtcgtagcg ctcgaggtgg tggaggatcg cgccggcgat
    19441 cgggcgcagc gcgggcacct gctcgacgag ccggtagccg atccgcgggt gcaactcgat
    19501 aagcgtccgc tcctccggcg tgagcgccgc cggcttcagg aggatgcgct cgctgatgcc
    19561 gatcttgccc acgtcgtgga gcagcgaccc gaagacgagc tcctcgcgcc tgcgcgagtc
    19621 gaccccgagg cggtcggcca ccgccgccac gtagccggag acctcctccg agtgtcctcg
    19681 gaggaacggg tccttcgcct ggatcgcctc cgcaagcacg cggacggtgc cgaggtaggc
    19741 gccgcgcagc tcgccctgga ggcggctgtt gtcgagcacg gcgccggcgt ggtcgccgag
    19801 cgcgatcagc acgtcgtcgt cccactcctc gaagccaccg tcgcggttgg cgcagaccac
    19861 cacgccgctg aagcgatcgc ggatgtagat cgggatcgcg acgaggttct cgatctcgct
    19921 gtcggcctcc gagccgcgct cgccgtcctc tgactggcgc ggggcgtcct cgcggacggt
    19981 ctcgtcgcgg tcgagcacac gtccggcgaa gcgttgcgcg atcgccgagt gctccgggtc
    20041 gttgtcgaag ccctggtagg cgaccaggtc gaggtcgccg tcgccgtcct cgtcggtgcg
    20101 cgagagcagc aggcccttct cggactcgag cagggtcacg gcgacgcgca ggactagcgc
    20161 gcggatgtcg ctcgcgtcgc cgagcacgcc gccctcgtgg tgggcctcga tgagctgggc
    20221 gcggaactcg cggtgccact cgcgctcggc gtcgcgctcg gtctcgatgc gctgcgcgcg
    20281 ccccgactcg cgctcggcgc gctcctcggc ctcggccacg cgcttctcga gctcgtcggt
    20341 gcggccgatg cggtcggcga tcgtgcgccg gtcgaggcgc cagccgatca gagcgccgag
    20401 gccggtcgcg agcgcgagca ggacgaggcc cagcgccagg ccgcgctcgt tgctgagcgc
    20461 gccgtctacg gcggcggtga tgatcgtgta ggccgcgatc gtcacgaaca gcaccactgc
    20521 cgtcgtgaaa gcgcgtgcgt gcgtcagtcc gagcgcgtcc ctccgcccgc ggggtacgtc
    20581 catggagccg taggtacccc ggttcgcgtg cgggtatgcg cggagtcagt ccgcgagcag
    20641 cggctcgagg ccggcgcgga cctcgtccgg catggctttc ttggcctttg tggccgggtc
    20701 gatgaagacg tggcgcatgt ccccgctggc caccgggcgg ccctcgacac tcgcctcgat
    20761 gcgggtgctg accgcggtgt tgccgaggcg cgcgatggtc acgtcgaagt cgacgttgtc
    20821 gtcgaagccg gcagcgccga ggaagcgaac ggtggcctcg gcgacgacca tgtccgtgcc
    20881 ggcggcgagc atttcggcgt aatcgcccgc ccggtcgcgc cacagctcgg tcatcacgac
    20941 gtcgaagtag gcgagccagt tggcgttgaa cacgaccccc tgcgggtcgc actcgccgaa
    21001 gcggacgcgg atggtgtggc ggaagctctg ggccatgcaa tacatcttgc agcctgaggc
    21061 cccttccgtc cggcccgctc cttagcttgc cgaacacatg ttcccccttc tccgcacccg
    21121 cgccgcccgc gtcctagacg tcgcgatcga gttcgcaacc ctgggcgagt accgcctgga
    21181 ggacccgatc gccccggtcg ccccggcgcg cggagcgacc cgcggaaccg agccggccga
    21241 gcgcctccac ggcgcgtcgg ttcggccggc cgcgcggctc gtggcgccgg caagcgcggc
    21301 agccgtccgg ctccgccccg cccccgctcc tgcggttccc ggccccgtgc gcacgcgccc
    21361 gaagcccgcg tcaccgctcc aggacgtggg cgcgatcaag cagctcgcgc tcgatttcga
    21421 cggcgcgctg ccgcgcccgg cggtacgcac ccgcggcgga gcggcacccg cagcaccgca
    21481 gccctgcgag agcccgcgcc gctagtaggc gcccaagcga ccgcgccgcc aggcgcaccg
    21541 agcgccgagt ggcccgacgc ggcggtccca agccggccgc ggctcgaccc ggcgcgcccc
    21601 aagccggctc cgcggctccg cccggcgcgc cccaagccgg ctccgcgact ccacctggcg
    21661 cgccccaagc cggctccgcg actcgaccca gtcggcgcac gcgagccctc ccgcatcgat
    21721 tggagccttt ggtcgctatt tggtgctcaa ccctccaatc gatgaggagc tcgatgacga
    21781 gcgccgtgag tctggcgccg acgactggtt aggcgagcag gccgcgctgc tcgagctcgc
    21841 cgcggagcca gcggatcggc tccgggttct tggcctcgct ggcgccgtcg acctcgatga
    21901 accgctgaaa gagagcgtgg tcggggaccc gatcgagcat ctcctcgaac gcgccgcgat
    21961 cgagctcgct gctcttcttc atccccttcc agaggtggta tgggcgatcg tggaactcga
    22021 tcgtcgcaag caggcgctcg tcatcggtgt agcgctcggc gaagcggcgg gcgcgggtgg
    22081 cgtggtgatt tcggccctgg cgagggagcc agttgcgcac gcggtacttg aacgagtcgt
    22141 ggacaagggc gatgaagcgc aggtccgcgc gccgcgcgcc ggtctcgcgg tcggagtcga
    22201 tctcgtcgag gagctgagcc acgtggatgc cgaccgcacc ctcgggatgc gcctcccgcg
    22261 gcttccccca cgcgagcccc gcgagcagct ccggatcgcc ggtcacgcgc tcctcgagct
    22321 cgttctcggg cgcgaagccc ggaatgaggc gcgcggcctc gccggcgggg gtggccgaag
    22381 ggctgtcgcc cgcgggcgcc cccgacgctg cttcaccggc ccgcggtgcg cccgagggct
    22441 cggcggcggg tgcccccgcc tggggctccc cggaggcttc acccgcctcc ggcgcggtca
    22501 ttcgacccgc tcggcgtacc agtctccgcc ctcggtgcgg gcgagcacca cgcgagcgga
    22561 tccgcctgag cccgtggcga ccagatgggc ggcgtcctcc gccatgccgg gccggtcgtc
    22621 gacgagctcg agctcgatgc gctcccagct cgaaccgggg cgctgctcgt ccagcgccgt
    22681 ccgagcggtg cgctccagca cggcgcggtc ccagccacgg tcgctcgcgc gcgcctgctc
    22741 gagcgcgcga tcggcggcca cgatgcgcgc agcggcctcc gggcggttcg cctcccggcg
    22801 ggcacggatc aagccaccga tgaacagcac cagcagcaga actccgatga cgatgagcac
    22861 gatcgcgagg acggacatcg cggcgagtct aggcgcagcc cccgcgggat cccggtgtag
    22921 ggcgcgaccg gacctggcga ccgtgccgga gcgtggacgg gtggtgcggg atgcgcggag
    22981 ccgtggtgcg ggatgcgcgg agccgtggtg cgggatgcgt gcgcaacggt gcgccggcgc
    23041 cggcgcgatg cgggaggtgc cagggcgagc cgaaaccgcg cgcgccgggg ccacgcggga
    23101 accacgcggc accgagccgg ggtgcgggcg gtgctccgag gggggctgct gagggagcct
    23161 gcgaccgtac ccccgaggag cactgcacgc accccggcaa gcgcggcggt caggaggagg
    23221 cggccggagg ggacgcggcg gtcaggagga ggcggccggt cgtagaacga caaacgggcc
    23281 gcccaaagga cggcccgttc ggtcatttac tccggcggcg acctactctc ccaggccctt
    23341 tcaagccaag taccatcggc gctgtggggc ttaacttctc tgttcggaat gggaagaggt
    23401 gtgaccccca cgctatggcc accgaaaggc tgcgagaccg gcgctcctga cgagcaccgc
    23461 aggccccgcc gggccttgaa aaccgcacag ttggctgcca catcgggcaa aaagaaatcc
    23521 gtcaagccct cgaagcatta gtaccggttc gctaagcgca ttactgcgtg tacacgaccg
    23581 gcctatcaac ctggtggtct accagggctc ttactccctc gaagggatgg gagagctcat
    23641 ctcgaggtgg gcttcccgct tagatgcctt cagcggttat cccgtccaga cgtagctaac
    23701 cagcggtgcc cttggcagga caactggtac accagaggtc tgttcatccc ggtcctctcg
    23761 tactagggac gactcctctc aactctccaa cgcgcacggc agatagggac cgaactgtct
    23821 cacgacgttc tgaacccagc tcgcgtaccg ctttaatggg cgaacagccc aacccttggg
    23881 acctacttca gccccaggat gcgacgagcc gacatcgagg tgccaaaccg ggccg
//
LOCUS       DASVPL010000022         6648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_158340, whole genome shotgun sequence.
ACCESSION   DASVPL010000022 DASVPL010000000
VERSION     DASVPL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158340"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VF517_01215"
     CDS             <1..1312
                     /locus_tag="VF517_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8051582.1"
                     /translation="LITVGTLTWLKQIGIGDKYVQDDAPDPELAYQRAFTIDALSNLV
                     LLAVALVALPVLALAYGHWEIVAPGAVLAVAVAAQSFKTPVWIFYRDLRYGKQRAIEA
                     VDPLVSFVVTIGLAAAGAGYWSLVIGYAAGIAATGAVAVAASPYRLALRYDRAKLREY
                     FEFSWPIFVAGASALLIPQLSLLVGTWELGLAGAGVIALAGSIGSYTDRIDELVTWTL
                     YPAICRVRDRSDVLFEAFAKSNRLALMCGVPFGVGVALFAADLVDHGLGDEWQGAVGM
                     LQVFGLLAAAHHVGFNWVAFFSARGETRPMAVTAGVVLVAFLAITLPFTYAWGLDGYA
                     AGMAVVTAISLAMRGFYLRRLFRGFRLLPHALRAVAPTVPGAAAVLAVRVLLDLDRTV
                     AVALGELGLFVAVVLGATVVLERALLRELVAYARGRGAPLVQTA"
     gene            complement(1410..2168)
                     /locus_tag="VF517_01220"
     CDS             complement(1410..2168)
                     /locus_tag="VF517_01220"
                     /EC_number="1.1.1.333"
                     /inference="COORDINATES: protein motif:HMM:NF005912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenylphospho-beta-D-erythro-pentofuranosid-
                     2-ulose 2-reductase"
                     /protein_id="HEX8051583.1"
                     /translation="MRDATGAVQSVLVLGGTSEIGLATAKRLVRERVRRVVLAVRDPA
                     RAETSAAELRALGTEVEIVGFDALDFGSHGAFVEDVFDRFGDIDVALVAWGVLGDQER
                     LATDHDAAVEAVEVNYTGVVSVSVPLVKRMRRQGHGTIVYLSSVAGERARASNFVYGS
                     TKAGMDAFAQGLGDSLAGSGVQVMVVRPGFVKTKMTEGLDPAPLATDPDSVADAIAAG
                     LRRNAHTVWAPGPLRFVMSALRHVPRPIFRKLDL"
     gene            complement(2165..3490)
                     /locus_tag="VF517_01225"
     CDS             complement(2165..3490)
                     /locus_tag="VF517_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8051584.1"
                     /translation="MLTGWGRTAPSRAEVVPASAEAIVSALGSAGPRGVLARGLGRSY
                     GDAAQNAGGRVLLMEGLAGVHDVDIAAGVVTVDAGVSIETLTRALLPLGLFVHVTPGT
                     WHVTIGGAIAADVHGKNHHADGSFCDHVLSFELVKPDGSQVTVRPGDELFDATAGGMG
                     LTGVIVAATLRMLKVDSPNMVVDRERASDLDDVMARMSEHDDDYRYSVAWIDCLARGG
                     SLGRSVLIRGDHAPASHNGNGNGGHFGGGLVRLSAPAWTPPGLLRRSTIRIFNEAFFR
                     AAPRREVGRVEGLREFFYPLDAVGAWNRIYGPSGFLQYQLVVPDGAEDALRESLEHLS
                     GAGTPSFLAVLKRFGEQRTGIISFPMKGWTLALDIPAATPGLAPLLDGLDELVAEAGG
                     RVYLAKDSRLRPDVLAAMYGRLGDWRAVRERVDPHGVMRSDLARRLELA"
     gene            complement(3495..4445)
                     /locus_tag="VF517_01230"
     CDS             complement(3495..4445)
                     /locus_tag="VF517_01230"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX8051585.1"
                     /translation="MAEGVETVTPRAAAPGRVTSFDGTWRGTLPGGILRALRPKQWLK
                     NVLVAAAPGAAGVLTEGDVLVDVGLAFVAFCMVSSGTYLLNDVRDVEADRLHPRKRFR
                     AIAAGTVPVRLAVALAFVMLAGGFAVAAAVRVELFAVVVSYVTLTSAYTLWLKHVEVV
                     DIVAIASGFILRAVAGGAAVDVPLSRWFIIVASFGSLFIVAGKRHGEHIDLDETRGEV
                     RATLAGYSRDYLKYVWTMASGVTIAAYCLWAFEMAPRGSEVPLYELSIVPFVTFMLRY
                     AQLLEAGHGEAPEDVVLGDRVLAVLAGVWIALFGAGVYFL"
     gene            complement(4456..6330)
                     /gene="asnB"
                     /locus_tag="VF517_01235"
     CDS             complement(4456..6330)
                     /gene="asnB"
                     /locus_tag="VF517_01235"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX8051586.1"
                     /translation="MCGIAGFVAAPGRAPERAALERMARALEHRGPDDSGIAVEGQVG
                     LVHRRLAIVDPSPAGRQPMADASGRWLVTYNGEAFNHMELRAELPGVAWRGGSDTETL
                     VEALARWGEGAVARCNGLFAYAALDRERGRLLLVRDRFGVKPLYVARHAGALWFASEI
                     RALLEAGVEREVRRDVLAHAVERGWANGRRTPLAAVERVMPGTLVEVDLETLRTRERV
                     WYDPLDAVGAARGPAAAAPRGPAAASPRGPAAASPAAPPPPALDDALRASVRRRLMSD
                     VPLGTMCSGGLDSGLITALAAEAGTVHAFNARIVDQPEWDEGAYARAVAEHVGVELHT
                     VEMTAADWRAELVDVVRHVEYPLTHESSVPMAAIARLAHERGIKVLLSGEGADELFGG
                     YSWLHHHAFADFRARRRAPEAAARAALRALQSRGVLAGRAIPDPLPGPSGEVNAYERG
                     RVERALAAYSHHRGPRRRLEAGLAADLSTYLPHLLNRQDKTTMRHSVETRVPFLDPEV
                     VAAALAMPLERRVEPERKAPLRALGRRLLPAAVVDREKVGFGFDVARYLGGSVRPEFL
                     RDGLLREELGVEPAAWDANVAAMSAALAPVTAEIWLRGVVRGDSTDDVQAQLWAPGRP
                     "
     gene            complement(6337..>6648)
                     /gene="msrB"
                     /locus_tag="VF517_01240"
     CDS             complement(6337..>6648)
                     /gene="msrB"
                     /locus_tag="VF517_01240"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8051587.1"
                     /translation="PPFTGEYVYTKENGVYRCAACASELFSSETKFDSGTGWPSFYEP
                     MSEGGVEMREDRSHGMVRIEAVCANCGSHLGHVFDDGPNPTGQRFCINSAALDLDAGG
                     D"
BASE COUNT          911 a         2476 c         2409 g          852 t
ORIGIN      
        1 gctgatcacc gtgggcacgt tgacctggct caagcagatc gggatcggcg acaagtacgt
       61 gcaggacgac gcgcccgacc ccgagctcgc ctaccagcgc gcgttcacga tcgacgcgct
      121 ctcgaacctg gtcctgctcg cggtcgcgct ggtggcgctg ccggtgctcg cgctggccta
      181 cgggcactgg gagatcgtcg cgccgggcgc cgtgctggcg gtggcggtgg cggcgcaatc
      241 gttcaagacg cccgtctgga tcttctaccg cgacctccgc tacgggaagc agcgggcgat
      301 cgaggcggtg gacccgctcg tgtcgttcgt ggtcacgatc ggcctcgcgg ccgcgggcgc
      361 cggctactgg tcactcgtga tcggctacgc ggccggcatc gcggccaccg gggcagttgc
      421 ggtcgccgcg tcgccgtacc ggctggccct gcgctacgac cgcgcgaagc tgcgcgagta
      481 cttcgagttc tcctggccga tcttcgtcgc gggcgccagc gcgctgctga tcccgcagct
      541 ctcgctgctc gtcggcacct gggagctggg cctcgccggc gcgggcgtga tcgcgctggc
      601 cggctcgatc ggcagctaca ccgaccggat cgacgagctc gtcacctgga cgctctaccc
      661 ggcgatctgc cgcgtgcgcg accgcagcga cgtgctgttc gaggcgttcg ccaagtcgaa
      721 ccggctggcg ctgatgtgcg gggtgccgtt cggcgtcggc gtggccctgt tcgccgccga
      781 cctggtggac cacggactcg gcgacgagtg gcagggcgcg gtcgggatgc tccaggtctt
      841 cggcctgctg gcggcggcgc accacgtggg cttcaactgg gtcgcgttct tcagcgcgcg
      901 cggtgagacg cgcccgatgg cggtgaccgc gggcgtggtg ctcgtggcgt tcctggcgat
      961 cacgctgccg ttcacctacg cgtggggcct cgacggctac gcggccggga tggcggtggt
     1021 caccgcgatc tccctggcga tgcgcggctt ctacctgcgg cgtctgtttc gcgggttccg
     1081 gttgctgccg cacgcgctgc gggcggtggc gccgaccgtg ccgggcgccg cggccgtgct
     1141 cgctgtgcgg gtgctgctcg acctcgaccg cacggtggcc gtggctctcg gggagctggg
     1201 gctgttcgtg gcggtggtgc tcggcgcgac ggtggtgctc gagcgggcgc tgctgcggga
     1261 gctcgtggcg tacgcgcggg ggcgtggggc gccgctggtc caaaccgctt aacggcggca
     1321 ctcaggggag gcgggatcgg ccgcgctcat cgattggagg gttagccggc tataggcggc
     1381 aaaaggctcc aatcgatggg cgctccccgc tagaggtcga gcttgcggaa gatcgggcgg
     1441 ggcacgtggc gcagcgctga catgacaaag cgcagcggcc ccggcgccca gaccgtgtgg
     1501 gcgttgcggc gcaggccggc cgcgatcgcg tcggccaccg agtccgggtc ggtcgccagc
     1561 ggggccgggt cgaggccctc cgtcatcttc gtcttcacga agccgggccg gaccaccatc
     1621 acctgcacac cgctgccggc gagcgagtcg ccgagaccct gggcgaaggc gtccatcccc
     1681 gccttggtcg agccgtagac gaagttcgag gcccgggcgc gctcgcccgc gaccgacgac
     1741 aggtacacga tcgtcccgtg cccctggcgg cgcatcctct tgaccagcgg gacgctgacc
     1801 gacacgacgc cggtgtagtt gacctcgacc gcctcgacgg ccgcgtcgtg gtcggtcgcg
     1861 agccgctcct ggtcgccgag aacgccccac gcgacgagcg cgacgtcgat gtcgccgaaa
     1921 cggtcgaaga cgtcctccac gaaggcaccg tgagagccga agtcgagcgc gtcgaagccg
     1981 acgatctcca cctcggtgcc gagcgcgcgc agctcggcgg cggaggtctc ggcgcgggcg
     2041 ggatcgcgca cggcgagaac gacacgccgg acgcgctcgc gcacgagtcg cttcgccgtc
     2101 gcgaggccga tctcggacgt gccgccgagc acgagcaccg actggaccgc tccagtcgcg
     2161 tccctcacgc gagctccagg cgccgcgcga ggtccgagcg catcacaccg tgcgggtcca
     2221 cgcgctcgcg tacggcgcgc cagtcgccga gccgtccgta catcgcggcg agcacgtccg
     2281 ggcgcagccg cgagtccttc gcgaggtaca cgcggccgcc ggcctcggcc accagctcgt
     2341 cgaggccgtc gagcagcggc gccaggcccg gggtggcggc ggggatgtcg agtgcgagcg
     2401 tccagccctt catcggaaac gagatgatgc cggtccgctg ctcgccgaag cgcttgagca
     2461 ccgcgaggaa cgacggcgtg ccggcgccgc tgaggtgctc gagcgactcg cgcagcgcgt
     2521 cctcggcccc gtccggcacg acgagctggt actggaggaa cccgctcggc ccgtagatcc
     2581 ggttccaggc gccgaccgcg tcgagcgggt agaagaactc gcgcaggccc tcgacccggc
     2641 cgacctcgcg ccgcggcgcg gcgcgaaaaa aggcttcatt gaaaatgcgg atggtggagc
     2701 gccgcaagag cccgggcgga gtccaagcag gtgccgacag acgaacgagc cctcccccga
     2761 aatgcccgcc gttgccgttg ccgttatgcg aagccggcgc atgatccccc cggatcagca
     2821 ccgaccgccc gagcgacccc ccgcgcgcca ggcagtcgat ccacgccacc gagtagcggt
     2881 agtcgtcgtc gtgctcggac atgcgcgcca tcacgtcgtc gaggtccgac gcgcgctcgc
     2941 ggtccaccac catgttcggc gagtcgacct tcagcatccg cagcgtggcc gccacgatca
     3001 cgccggtgag ccccatcccg ccggccgtgg cgtcgaacag ctcgtccccc ggccgcacgg
     3061 tcacctgcga gccgtccggt ttcacgagct cgaacgacag gacgtggtcg cagaacgagc
     3121 cgtcggcgtg gtggttcttg ccgtgcacgt cggcggcgat cgcgccaccg atcgtcacgt
     3181 gccaggtccc cggcgtgacg tgcacgaaca gcccgagcgg cagcagcgcg cgcgtgagcg
     3241 tctcgatgct cacgccggca tccaccgtca cgacccccgc cgcgatgtcg acgtcgtgta
     3301 cgccggcgag cccctccatc aggagcaccc ggcccccggc gttctgcgcc gcatcgccgt
     3361 agctgcgccc cagcccccgg gcgagcacgc cccgcgggcc ggccgagccg agcgcggaca
     3421 cgatggcctc ggcggatgcc gggaccacct ccgcacgcga gggcgccgtg cggccccacc
     3481 cggtcagcac tgcctcagag gaagtagaca ccggctccga acagggcgat ccacacgccc
     3541 gcgagcaccg cgagcacgcg atcgccgagc acgacgtcct cgggcgcctc gccgtgcccg
     3601 gcctcgagca gctgcgcgta gcgcagcatg aaggtcacga acggcacgat cgagagctcg
     3661 tacagcggca cctcagagcc gcgcggcgcc atctcgaacg cccacaggca gtacgcggcg
     3721 atcgtcacgc cggaggccat cgtccacacg tacttgaggt agtcgcgcga gtagcccgcg
     3781 agcgtcgcgc ggacctcccc gcgcgtttcg tcgaggtcga tgtgctcacc gtggcgcttg
     3841 cccgccacga tgaagagcga gccgaacgag gcgacgatga tgaaccagcg cgagagcggc
     3901 acgtcgacgg cggcgccgcc cgccacggcg cgcaggatga accccgacgc gatcgcgacg
     3961 atgtccacca cctccacgtg cttgagccag agcgtgtagg cgctggtgag ggtcacgtag
     4021 gagacgacca ccgcgaacag ctctacgcgg accgcggcgg ccaccgcgaa gccgccggcc
     4081 agcatcacga aagcgagggc gaccgccagg cgcaccggca cggtgcccgc cgcgatcgcg
     4141 cggaagcgct tgcgcggatg gaggcggtcg gcctcgacgt cgcgcacgtc gttcaggagg
     4201 taggtcccgc tcgagaccat gcagaacgcc acgaacgcga gccccacgtc gacgagcacg
     4261 tcaccctcgg tgagcacgcc ggcggcgccc ggcgcggcgg ccacgaggac gttcttcagc
     4321 cactgcttgg ggcgcagcgc gcgcaggatc ccgcccggga gcgtgccgcg ccaggtcccg
     4381 tcgaacgacg tgaccctccc tggagcggcc gcccgcggcg tcaccgtttc gactccctcc
     4441 gccaccgcgc cgattctacg ggcgcccggg cgcccagagt tgcgcctgca cgtcgtccgt
     4501 cgagtccccg cggaccacac cgcgcagcca gatctccgcg gtgaccggcg cgagtgccgc
     4561 gctcatcgcc gccacgttcg catcccacgc cgcgggctcg acgccgagct cctcgcggag
     4621 cagaccgtcg cgcaggaact cggggcgcac ggacccgccg aggtagcgcg ccacgtcgaa
     4681 cccgaagccg accttctcgc ggtcgaccac cgccgccggc aggagccggc gcccgagcgc
     4741 gcgcagcggc gccttgcgct cgggctcgac gcgccgttcg agcggcatgg cgagcgcggc
     4801 ggccacgacc tcggggtcga ggaacggcac gcgcgtctcg accgagtggc gcatggtggt
     4861 cttgtcctgg cggttgagga ggtgcggcag gtaggtggac aggtcggccg ccagccccgc
     4921 ctcgagccgc cggcgcgggc cgcggtggtg ggagtacgcg gccagcgcgc gctccacgcg
     4981 cccacgctcg tacgcgttga cctcgcccga ggggccgggc agcgggtcgg ggatcgcccg
     5041 gccggcgagc acgccgcggc tctggagcgc gcgcagcgcg gcgcgggccg cggcctcggg
     5101 cgcgcgccgg cgcgcacgga aatccgcgaa cgcgtggtgg tgcagccacg agtagccgcc
     5161 gaacagctcg tcggccccct cgccgctcag cagcaccttg atcccgcgct cgtgcgcgag
     5221 gcgcgctatc gcagccatcg gcaccgagct ctcgtgcgtg agcgggtact ccacgtgccg
     5281 caccacgtcg acgagctcgg cgcgccagtc ggccgcggtc atctcgaccg tgtgcagctc
     5341 cacccccacg tgctccgcga ccgcgcgcgc gtaggcgccc tcgtcccact cgggctggtc
     5401 cacgatccgc gcgttgaacg cgtgcaccgt gccggcctcg gccgcgagcg cggtgatgag
     5461 accggagtcg agcccgccgg agcacatcgt gccgagcggg acgtcggaca tgagccggcg
     5521 gcggacggac gcgcgcaggg cgtcgtcgag ggccggcggc ggcggcgcgg ccggcgaagc
     5581 cgcggcgggt ccgcgaggcg aagccgcggc cggaccgcgg ggcgccgccg cggccggacc
     5641 gcgagccgcc ccaaccgcgt cgagcgggtc gtaccagacc cgctcgcgcg tgcgcagcgt
     5701 ctccaggtcc acctccacga gcgtccccgg catcacgcgc tccacggcgg ctagcggggt
     5761 gcggcgcccg ttcgcccagc cgcgctccac ggcgtgggcg agcacgtcgc ggcgcacctc
     5821 gcgctccacc ccggcctcga gcagcgcgcg gatctcgctc gcgaaccaga gcgcgcccgc
     5881 gtggcgcgcc acgtacagcg gcttcacgcc gaagcggtcg cgcacgagca gcaggcgccc
     5941 gcgctcgcgg tcgagcgccg cgtacgcgaa caggccgttg cagcgcgcca cggcgccctc
     6001 gccccagcgc gcgagcgcct cgaccagcgt ctcggtgtcg ctaccgccgc gccacgcgac
     6061 gcctggcagc tcggcgcgca gctccatgtg gttgaacgcc tcgccgttgt aggtgacgag
     6121 ccagcgcccg gaggcgtcgg ccatcggctg gcggccggcc ggcgaggggt ccacgatcgc
     6181 gagccgccgg tgcaccaggc ccacctgccc ctcgactgcg atcccggagt cgtcgggccc
     6241 gcggtgctcc agcgcccgcg ccatccgttc gagcgcggcg cgctccggcg cgcggcctgg
     6301 ggcggccacg aagccggcta tgccgcacac gccgggctag tcgccgccgg cgtccaggtc
     6361 gagcgcggcg gagttgatgc agaagcgctg cccggtcggg ttcgggccgt cgtcgaacac
     6421 gtgcccgagg tggctgccgc agttcgcgca cacggcctcg atccgcacca tgccgtggct
     6481 gcggtcctcg cgcatctcca cgccgccctc ggacatcggc tcgtagaagc tcggccagcc
     6541 ggtgccggag tcgaacttgg tctccgagct gaacagctcc gaagcgcacg cggcgcagcg
     6601 atagacgccg ttctccttcg tgtagacgta ctcgcccgtg aacggcgg
//
LOCUS       DASVPL010000023         2788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_158513, whole genome shotgun sequence.
ACCESSION   DASVPL010000023 DASVPL010000000
VERSION     DASVPL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158513"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VF517_01245"
     CDS             complement(<1..632)
                     /locus_tag="VF517_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX8051588.1"
                     /translation="MTRASGERGQTSILLLGVLATLIAISLMLAAFGQALGGRGRVQR
                     IADLAAISGAHAMRESYPRLFEPPQLAPNVPNPRHLSRAQYLALARAAALRAARRNGQ
                     ALLPADIQFPDAASMAPTRIAVSVRAQTDVRISQDRRGGRRRIPVKAHAEAELSPPAD
                     DGLGPRGHGEYTGPLAYRQGKPMRPDVAQAFDRMQRAAASAGVRLLVVSA"
     gene            complement(629..1222)
                     /locus_tag="VF517_01250"
     CDS             complement(629..1222)
                     /locus_tag="VF517_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051589.1"
                     /translation="MVAVLGLAPRAGASTVARAVAARLAAEDPCGAAILVTPDPPRSG
                     LGTAAASRLARTLAERGTEGPRPAGRLCVVNATEPLAPIVAQRSATVVADIPHGLPAE
                     GVVALADHVVLVCAPDVEPALAAAVESSLRGSAPAVSLVVNRALEGSPPGLQHAIAIP
                     ESRTAAQLTLACRGPRGPLAEPATELAERTLEVAWLR"
     gene            complement(1528..1851)
                     /locus_tag="VF517_01255"
     CDS             complement(1528..1851)
                     /locus_tag="VF517_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051590.1"
                     /translation="MSERGQASVELLGGLPAVILTALVTFQLLALGYSKVLAGNAAEA
                     AAIAAAADGDPRAAARQAVPGWSRARMRVESRGGRVWVRMRPPSPLRRVARVLEVEAS
                     AAVGR"
     gene            complement(1848..2210)
                     /locus_tag="VF517_01260"
     CDS             complement(1848..2210)
                     /locus_tag="VF517_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX8051591.1"
                     /translation="MHGAAKIRRGDERGQASAELVAVLPLVVLVAAIGWQLALAGYSA
                     WMCANAARVAARAEAVGGDREQAARSALPRSLERGLRVERTAAGAVRVELRVPLILRH
                     WRSPVAVSASAALPQGTR"
BASE COUNT          372 a         1074 c         1020 g          322 t
ORIGIN      
        1 aaggcgctga ccacgagcaa gcggacgccg gcgctcgccg cggcgcgctg catccggtcg
       61 aacgcctggg cgacgtcggg ccgcatcggc ttgccctgcc ggtacgcgag cggcccggtg
      121 tactcgccgt ggccacgcgg cccaaggcca tcatccgcgg gcggggacag ctcggcctcg
      181 gcgtgggcct tgaccgggat gcggcggcgg ccgccgcgcc ggtcctgcga gatccggacg
      241 tcggtctgcg cacgcacgct caccgcgatg cgcgtgggag ccatgctcgc ggcgtccggg
      301 aactggatgt ccgccgggag gagcgcctga ccgttccggc gcgccgcccg cagcgccgcg
      361 gcgcgcgcga gcgcgaggta ctgcgcgcgc gagagatggc gcgggttcgg gacgttcggc
      421 gcgagctgtg gcggctcgaa cagacgcggg tatgactcgc gcatggcgtg ggcaccgctg
      481 atcgcggcga ggtcagcgat ccgctgcacg cgcccacggc cacccagcgc ctgaccgaag
      541 gcggcgagca tgagcgagat cgcgatcagt gtggccagta cgcccaggag gaggatcgag
      601 gtctggccgc gttcgccgga agcgcgcgtc atcgcagcca cgccacctcg agggtccgct
      661 cggccagctc ggttgcgggt tccgccagtg gcccgcgcgg cccgcggcac gccagcgtga
      721 gctgggccgc cgtgcgtgac tccgggattg cgatcgcgtg ctggaggccc ggcggcgatc
      781 cctccagcgc gcggttgacc accagcgaga ccgccggcgc gcttccgcgc aggctgctct
      841 ccaccgcagc ggcaagcgcc ggctcgacgt cgggggcgca gacgagcacg acgtgatccg
      901 cgagcgccac gacgccttcc gccggcaacc cgtgcggtat gtcagcgacc accgtggcgg
      961 aacgctgcgc cacgatcgga gcgagcggct cggttgcgtt caccacgcag aggcgtcccg
     1021 cgggtcgcgg accttcggtg ccgcgctcgg ccagggtgcg cgcgagtcgc gaggcggccg
     1081 ccgtcccgag cccgctgcga ggcggatcgg gcgtgacgag gattgcggcc ccgcacgggt
     1141 cttccgcggc aagccgggcc gccacggccc gcgcgaccgt ggacgcaccc gcgcgcggag
     1201 cgaggccgag gaccgcgacc acggggggcc gcggctctgg ctcggcgcgt atcggcggcg
     1261 cgagactgag gggtgggcgc gcgtcggtgg accggtggga acggcgtggc gcggggtccg
     1321 cttccggcgc ggggatggga cggagccgct gcagcgccgg cgaccgctgc cggccgcgct
     1381 cggccgtggc tgcggcaaca gggcattcgg cagcgcgtgc ctccgcttct gcctcctccc
     1441 ccgccgcggg cggcgtgagt agccaccccg ccgcgcgagc gatcagcgcc ggcaccgaga
     1501 ccgcgcggag ctgctgcgac cacgcgctca tcggcccacc gccgcgctcg cctcgacctc
     1561 gagcacgcgc gccacccggc gcagcggcga cggcgggcgc atccgcaccc acacgcgccc
     1621 tccgcgcgac tcgactcgca tccgcgcacg cgaccagccc ggtaccgcct ggcgggcggc
     1681 cgcgcgcggg tcgccgtccg ccgcggccgc gatggcggcc gcctccgccg cgttgccggc
     1741 gagcaccttc gagtacccga gcgcgagcag ctgaaaggtg acgagggccg tgaggatcac
     1801 cgccggcaac ccgcccagga gctcgacact cgcctgccca cgctcgctca ccgcgtcccc
     1861 tgggggagcg cagccgacgc actcaccgcg accggactgc gccagtggcg caagatcagc
     1921 ggaacgcgca attcgacgcg aaccgcgccg gcagcggttc gctcgacgcg cagcccgcgc
     1981 tccagcgagc gcggcagcgc gctgcgcgcc gcctgctccc ggtcgccgcc caccgcctcc
     2041 gcgcgcgcgg ccacccgcgc ggcgttcgcg cacatccacg ccgagtatcc cgcgagcgcg
     2101 agctgccatc cgattgcggc cacgagcacc acgagcggca gcacggcgac cagttcggcg
     2161 ctcgcctggc cccgctcgtc accccgtcgg atcttcgccg ccccgtgcac gcacggacgg
     2221 tcccgcgatc cgtgttacgg ctcaagacgc gcgcgttaca agttcggcgc cggaacgtga
     2281 cgaaacgggg acagcccccg cggaccgggc ggcattcctg actttgcctc cctatgggtg
     2341 gtcaatccgg gaatgtcgac gccgggcggc gaggccggcg ggtgacgcga gcccggcggg
     2401 tcgcgccgag ctggccgtgg cgttcgagcc gctggtggcg gagcggccgc agcaagctga
     2461 gcgccacacg cggcggcctg cgcttccgct gaggccgcct ctccggcgtt cctgacttat
     2521 gggcccatat gacctaaaag tcaggaatgt ccaagatttc ttcccgtccc cccacccgag
     2581 cccggcgggg gcgcgagccc ggcgtgcggc gcgcccctac ccgtactcct gcgacgacct
     2641 ccaggtccag tacaccccat acgacgccag ccccagcagc gacagccatc cgatcacgcc
     2701 cagaccgccg ctgtcgaaca tccggttcgt cgccgcgaat accagcagcg ccagcccgat
     2761 cgacccgtat aggacctggc gctcgcgg
//
LOCUS       DASVPL010000024        15345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_119426, whole genome shotgun sequence.
ACCESSION   DASVPL010000024 DASVPL010000000
VERSION     DASVPL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15345
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119426"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VF517_01265"
     CDS             <1..432
                     /locus_tag="VF517_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051592.1"
                     /translation="LATVLAVFARPEFQRDDWRGAAEAIGPARADRALVVNPIAGGTP
                     LRLYLDNLEPYGEEFRPVSEIALVAVASRRPGETPRPPRPPSPAQPGFEVAERKETDT
                     FTLVLLRAPAPVAIASAGLTALKLEPSPDDLAVTLMQRPSR"
     gene            complement(429..1985)
                     /gene="selB"
                     /locus_tag="VF517_01270"
     CDS             complement(429..1985)
                     /gene="selB"
                     /locus_tag="VF517_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX8051593.1"
                     /translation="MAPRRDDRPPLTLGTAGHIDHGKTALISVLTGKNTDRLPEERSR
                     GISIELGYASLDLPSGRRLSVVDVPGHERFVRTMVAGASGVDLFLLCVASDDGVMPQT
                     REHLAVLRQLGIEHGVVAITKADVGEPELAAEEVAELVPGVPLVAVSSVERTGIDELV
                     AALDGVAARLPGRAGDDDGARLHVDRSFTLRGIGTVVTGTLWSGSIAAGDSVRILPRG
                     LEARVRTVQVHDEPVERAAAGQRVALNLGGIGWREVGRGDVVVAPDAEIAPGYLVDAA
                     VRLEPGARSLRGSTRVHVHHGTREAPARLVTLDGELRPGEEGFAQLRLEAPLVASAGD
                     RLILRQVAPPDTIGGGVVVDPRPRRHGARPEVVERLRARARGEPEPEAAPAPEPEATA
                     DEPPPLDDGALLIASLLRSDGDSPRTDADLAAAAGLDPRATAERLRQLERAGQAVRVA
                     RNLHIHPEPLAGLEARIVAICEREGSATIAGVRDELGTSRKYAQALLEHLDRTKVTRR
                     DGDAHVLRRR"
     gene            complement(2000..3475)
                     /gene="selA"
                     /locus_tag="VF517_01275"
     CDS             complement(2000..3475)
                     /gene="selA"
                     /locus_tag="VF517_01275"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX8051594.1"
                     /translation="MEKPAGSRELDPMAREALRSLPSVEELAARLDGVPHGVAVRAAR
                     EAIAARREAIADGVAPADGVAPAGGAAPAEGAAPAGGAGSADGAAAAGDLLADARARV
                     EATLRGTLRPVLNATGVIVHTNLGRAPLAEAAAAAAAEAGRGYSNLEYDLDAGARGSR
                     QSHAEAILRELTGAEAAIVVNNCAAAVLLAAAALAAGRELVVSRGQLVEIGGSFRVPD
                     VVAQSGARLVEVGTTNRTRPSDYERALGPDTGALMRAHQSNFRTVGFVEEVEIEELCG
                     AAARAGVPVIDDVGSGALAERVPELADEPSVRRSVAAGAAVVCFSGDKLLGGPQAGLM
                     VGSAAAIERCRTHPLARALRIDKLSLAALTATLRLYLDPQAALREVPTLRMLTATESE
                     LEARAESMRSAIADAGIAARTIRASGKVGGGALPLLELEGPVCAVDPAPLALDELAAR
                     LRRADPPVIARAHDGWLLLDPRTLDDAGAGMAAAALIRVFR"
     gene            complement(3429..4076)
                     /locus_tag="VF517_01280"
     CDS             complement(3429..4076)
                     /locus_tag="VF517_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX8051595.1"
                     /translation="MEVTVRLFAMLRERAGSSELTLELPDGARVGDALAALGELADGL
                     PLVMAVNREYAREDAPLDPGDELALIPPVSGGEAAVAHALVTAEPLSLDALAARVRDP
                     RAGAVVTFQGVTRDVERLDYEAYAEMAVGRIEAIVAEAIERHGLCAAAAEHRVGSVAL
                     GEPSVIVAVSAAHRGEAFAGAREIIDRVKAEAPIWKREVEGGEARWVEGARPDGA"
     gene            4108..5247
                     /locus_tag="VF517_01285"
     CDS             4108..5247
                     /locus_tag="VF517_01285"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX8051596.1"
                     /translation="MALAYRVALLDWLACAVGGAGEPATRAALGAAEGLEGRVAAAGT
                     AGHVLDFDDTYTPGLAHLSAPVAPAAVVLGGELGATVGEVVSAYATGFEAMAQLTRAN
                     HPGMRERGWHPTATCGAVGAAAAAAELLELDDGRRLAALRLALLATSGLRAAFGSDGK
                     SLQVGSAAAAGVRAARLAAAGASVGASAERGWEQAYGARWVEPEAAAGAIHANWIKAH
                     PCCLQTHGAIDAALASGASPGDGVEVTVHPVSLTAAEIDDPADGLQAKFSIPYLTAYA
                     LMRGAPTVESFRSIDDAVRVEARSIRVSTDASLLESEAVLSARGAVLARVEAAVGSPE
                     NPMTAERLAAKVGQLAGGRLDGALDDPSRDAAELARILPGGGHHP"
     gene            5231..6850
                     /locus_tag="VF517_01290"
     CDS             5231..6850
                     /locus_tag="VF517_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8051597.1"
                     /translation="MATTPEHKPSIAPIVGPDDPRRFTDSGIEIKTLYDETDVAPGLE
                     ERLGKPGEYPFTRGIHPDMYRKRTWTMRQYAGYATAAETNERFRYLIEHGSTGLSMAF
                     DLPTQLGRDSDDPLCEGEVGRTGVAIDTIDDMRRVFDQIPLDEVSTSMTINAPAAVLL
                     LLYELVGEEQGVPAEQLRGTTQNDILKEYIARGNFIYPPEPAMRLTTDLFAYCDEHIP
                     KWNTISISGYHMREKGCSAVQEVAFTLANAIAYVQAAIDAGLEVDRFGPRLAFFFNGH
                     NNVFQEVAKFRAVRRMWAQIMRERFGSENPKSQTIRFHTQTGGVTLAAQQPENNIVRV
                     ALQGFAAVCGGTQSLHTNGYDEALALPTERAARIALRTQQILAHESGATDAVDPFAGS
                     YFVESLTDEIQERAKELMSKVEELGGSVNAIQFVRAEVEESAWGYDQRYKNGQDIVVG
                     VNKYVTDDVDDVEILRVDPKTEAEQVGRLTAFKEDRNQEAVQKRLEEVRATARGDANL
                     LVPIRAALKERASIGEVCGVLREEFGEYHEA"
     gene            6993..7544
                     /locus_tag="VF517_01295"
                     /pseudo
     CDS             6993..7544
                     /locus_tag="VF517_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
     gene            7595..10015
                     /locus_tag="VF517_01300"
     CDS             7595..10015
                     /locus_tag="VF517_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8051598.1"
                     /translation="MRRGTAVHVHVALLALCALLIVAAPAAAAGKPKVRAEIKRTAYG
                     IPHIKASNYEGIAFGYAYALAQDNICELADIYTTVRAERSRYFDPQATYASRGNGATF
                     KNIDSDFFFQRAIDTKIVEGLIAKPPPEGPLPILKEAVRGYAAGYNKYLKRIGGPNGL
                     DDPRCKGKDWVKPITEMDVYRRFWQLGILASQGVAIDGIATAAPLAPGVPLLGGAGAA
                     ANPAMLDELGSRLGEELGIGSNAVGVGSEGTRSGGGLLLGNPHFPWDGSERFYQTHLT
                     IPGKLNVTGGSLLGVPIVLIGHTDKMAWSHTVSTARRFLIYELKLVPGSPTSYIHDGQ
                     VKQMKADNVTIQVRNPEGGTTPQTRTLYSSEHGSILTSILGLPVFPWTAERAYAMDDA
                     NASNFRYLNHFFEVNHAQSVRQVHAIENRYQGIPWVNTIAADKKGEAYYADIGSMPNL
                     PNEKLSACEVPVGTALRTAAGVFAIDGSRGECDPGQDPTAVAPGILGPDKQPFLFRRD
                     YVENSNDSYWLTNPKQPLEGYPTLIGPERTARALRTRLGLRIIQQRLDGTDGRPGTKF
                     GLADMTWAVFNNRQYAGELWRDEAVAMCNANASSMPSSKGPVNVADACPVLAAWNVRD
                     DLDSKGAILARRFINRARAVQGGPYRRAFDPADPVNTPSGLNTENTAVQQAFGDAVND
                     LRDAGIPLDAPLRGYQYEMRGDKAFPIHGGPGTDGVFNAINVSWSPPKGYPNIPHGSS
                     FVQVVHFPRKGCAEHRTILTYSQSTNPNSKHFSDQTSLFSKKRWVNPPFCASQLRKAK
                     GSTQRLAR"
     gene            complement(10026..12842)
                     /locus_tag="VF517_01305"
     CDS             complement(10026..12842)
                     /locus_tag="VF517_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8051599.1"
                     /translation="MFRRILVVVVAAATCAWVVPRDATADRRGGGVGADLPPAQAFAA
                     SDGGASRPHSGRVRALVTLRHSPMLRRADAATNRVAITGHLNERQVAVLRTGRHLGSL
                     ASHLEERRRRGLVDVRAAARAAAADQDHVAAAMRDVGAIILEHDLATGSVTALLPAQS
                     LAAVGALPNVRSVELVHVEADLSLQLSTAAIGAPAMWDAGHLGGRGASDTYPVDLAIL
                     HDKIEQEHPAFAGIHFQRPSDAPIGTSCGQRVSGCEHGTEVASMAISRGAAGCAACVS
                     SDSSRMGVAPRLDSVLDAQASGMADDTAWALGVGSFGIQGATDPAEVMSNSHGSVAEA
                     DDSGSLRRTDKLISAYGALIAYPAGNEGPARTVNANCIAYDTLCVGGFDHRGTVDPAD
                     DVVEDFSSRGPTPGGRKKPDLVAVSTSEFANQHWIRDGRLWSGGSGTSLAAPQGAGAA
                     ALLAGSGIAAPIAQKAILINSARQGRATPTSPMGTQAGWQPDWGWGALDLDAAVKERT
                     HFYMDDVAGGDAHFYRADVQAAGDRATLVWNRRALGCIDPGCDTTTLTLSNLELEQLD
                     PATGAVEARSASGIDNVEQVRSPATGPVIYKVKASSTVDGLPGEPYALASRRQITPLA
                     APRPRARVTLSTTVARPGEPVTVMAELSNPSPDLSAESASATIALPPGVAIDEGSATQ
                     PVGTLAPGASTVVSWTVRASGDAVNRIAVRSTASRYGETFGSEDQATFTSDGSPPSVA
                     IAVPARGVTDPAIPIAWSGTDAGAGLRDYTVEVATNDGAFSPWLTATSATAATYTGTP
                     GARYRFRVRATDRLGTTSGYATTDEVVVEAPRAEPQPVPRPPGPAIRTNPRLRVRSIA
                     RRGSRIELRGTIASDAFRRLTVELNAKSGTKRVRRNGAAFPQAGRFKLALRAPQRLRG
                     TLSLRYTGDERYEPATTLVKLRGL"
     gene            complement(12918..15308)
                     /locus_tag="VF517_01310"
     CDS             complement(12918..15308)
                     /locus_tag="VF517_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051600.1"
                     /translation="MSVVTACGSGTGCRSDAAAPHGDRAGVRLYGATVRVHDDSAPSV
                     VDTGQGRLDNGAWQRGTNAVGYRISDNVGLRRTRLYVDGNRLREDERGGCDFTRARPC
                     LDVPPDLYDVNTAGLPDGRHVVRVEGVDAAGNVGGMDREFHSDNTAPDAPRELRVEGG
                     DGWRQTNQFKLTWQNPASASPITIARYRLCSTATQQCTVGARQGGGINSITDLSVPAP
                     GHYTLRLWLEDQAGNVKEANWSDEVSLRFDNVAPGEAAPAKRNGWLNADEAANYEQDI
                     RMVDGAFQPVSGVVGYSVTTDGSEPDSVLDAVGETYRIDALPEGTTVFKARAVSGSGV
                     ASAAVGRSEIRVDKSIPTVSAALDPRADGWHREPVNVSITASDQDHLSGLEPAREHLP
                     IEDGAYIAYRLDGSVRQTVRGGVGRLAFADDGAHTLTYRAFDVAGNASIERAVSFKID
                     RTAPELVVFEAPDAADPRRVVVAASDRTSGVASATIEMQRLHGDGRWIELPTTQDGDR
                     FVATVDDESVERGVYQLRARVKDQAGNESAGDRRRDGSSATVDTAALRSGSKLSAALV
                     KQSTKKTKTVCSKKRPGKKRKCRKKATKNPGGELVGTLSVPFGKGAVARGTLESDTGA
                     PLGNAVIDVYAQSAATGSEFERVGAVRTDPKGAFEYVARAGTSRAIRFAFDGDSKHRS
                     SDGAVTLKVAAAATIAASHRTRRNGQSVTFRGKLRSLPIPSAGKVLDLQAFYRGKWRT
                     FATPRANAKAKWSYRYRFGATRGTVPYRFRVLIRPEAAYPYDRGYSETVRVVVRGP"
BASE COUNT         2202 a         5774 c         5261 g         2108 t
ORIGIN      
        1 ctcgcgaccg tgctcgcggt gttcgcgcgc cccgagttcc agcgcgacga ctggcgcggc
       61 gccgccgagg cgatcggccc cgcgcgggcc gatcgcgcgc tcgtggtgaa cccgatcgcc
      121 ggcggaaccc ccctgcgcct ctacctcgac aacctcgagc cctatggcga ggagttccgg
      181 cccgtgagcg agatcgcgct cgtcgccgtc gcgagccggc gccccggcga gacgccccgc
      241 ccgccgcggc cgccgagccc ggcgcagccc ggcttcgagg tggccgagcg caaggagacc
      301 gacacgttca cgctcgtgct cctgcgcgcg cccgcgccgg tcgcgatcgc atccgccggg
      361 ctcacggcgc tgaagctcga gccgagcccg gacgacctcg ccgtgacgct catgcagcgc
      421 ccgtcgcgct agcggcggcg gagcacgtgc gcgtcgccgt cgcggcgcgt gaccttggtg
      481 cggtcgaggt gctcgagcaa cgcctgcgcg tacttgcgcg aggtgccgag ctcgtcgcgc
      541 acgcccgcga tcgtcgcgct cccctcgcgc tcgcagatcg ccactatccg tgcctcgagg
      601 ccggccagcg gctcgggatg gatgtggagg ttgcgcgcca cgcgcaccgc ctggcccgcc
      661 cgctcgagct ggcgcagccg ctccgccgtc gcgcgcgggt cgagccccgc ggcggcggcc
      721 agatcggcgt ccgtgcgcgg cgagtcgccg tccgagcgca gcagcgacgc gatcagcagc
      781 gccccgtcgt cgagcggcgg cggctcgtcc gcggtcgcct ccggctcggg cgcgggggcg
      841 gcctccggct cgggctcccc gcgcgcccgc gcgcgcaacc gctccaccac ctcgggccgc
      901 gcgccgtggc gccgcggccg cgggtccacc accacgccgc cgccgatcgt gtccggcggc
      961 gcgacctggc ggaggatcag ccgatcgccg gcgctcgcga ccagcggcgc ctcgagccgt
     1021 agctgcgcga agccctcctc gcccggccgc agctcaccgt cgagcgtgac gagccgggcc
     1081 ggcgcctcgc gcgtgccgtg gtggacgtgc acgcgggtgc tcccgcgcag cgaccgcgcg
     1141 ccgggctcga gccggacggc cgcgtccacc aggtagccgg gcgcgatctc ggcatccggc
     1201 gccaccacca cgtcccccct cccgacctcg cgccagccga tccccccgag gttcagcgcg
     1261 acccgctgcc ccgccgccgc gcgctcgacc ggctcgtcgt gcacctgcac ggtccgcacg
     1321 cgcgcctcga gcccgcgcgg caggatccga accgagtcgc ccgcggcgat cgagcccgac
     1381 cagagcgtgc cggtcacgac ggtcccgatt ccgcgcaggg tgaacgagcg gtccacgtgc
     1441 aggcgcgcac cgtcgtcgtc gccggcgcgc ccgggcagcc gcgcggccac gccgtcgagc
     1501 gccgcgacca gctcgtcgat ccccgtccgt tccaccgacg acaccgccac cagcggcaca
     1561 cccggcacca gctcggcgac ctcctcggcc gccagctccg gctcgccgac gtcggccttc
     1621 gtgatcgcca ccacgccgtg ctcgatcccg agctggcgga gcacggcgag gtgctcgcgc
     1681 gtctgcggca tcacgccgtc gtcggatgcc acgcagagca ggaacaggtc gacgccggac
     1741 gcgcccgcca ccatcgtccg gacgaagcgc tcgtggcccg gcacgtcgac cacggacagc
     1801 ctcctcccgc tcggcagatc gagcgacgcg tagccgagct cgatcgaaat tccgcgcgac
     1861 cgctcctccg gcaggcgatc cgtgttcttg ccggtgagca cggagatcag ggctgtcttg
     1921 ccgtgatcga tgtgcccagc cgtgcccagg gtgagcggcg gccgatcgtc gcgtcgcggt
     1981 gccatttacg agcggatggc taccggaaga cgcgaatcaa cgcggccgcc gccatccctg
     2041 cgccggcgtc gtcgagcgtg cgcgggtcga gcagcagcca tccgtcgtgc gcgcgggcga
     2101 tcaccggcgg gtcggcgcga cgcaggcgcg cggccagctc gtcgagcgcg agtggcgcgg
     2161 ggtcgaccgc gcacaccggg ccctcgagct cgagcaacgg cagcgctccg ccgcccacct
     2221 ttccgctcgc gcggatcgtg cgcgccgcga tgcccgcgtc cgcgatggcc gagcgcatgc
     2281 tctcggcgcg cgcctcgagt tcggactcgg tcgcggtgag catccgcagc gtcggcacct
     2341 cgcgcagcgc cgcctgcggg tcgaggtaca ggcgcagcgt ggcggtgagc gccgcgagcg
     2401 agagcttgtc gatccgcagc gcgcgcgcca gcgggtgcgt gcggcagcgc tcgatggcgg
     2461 ccgccgagcc caccatcaac ccggcctgcg gcccgccgag cagcttgtcg ccggagaagc
     2521 agaccaccgc cgcgcccgcc gccaccgagc gccgcacgct cggctcgtcg gccagctccg
     2581 gcacgcgctc ggcgagcgcg cccgagccga cgtcgtcgat caccggcacg cccgcgcgcg
     2641 cggccgcgcc gcacagctcc tcgatctcga cctcctccac gaagccgacc gtccggaagt
     2701 tcgactggtg cgcgcgcatc agcgcgcctg tgtccgggcc gagcgcgcgc tcgtagtcgg
     2761 agggccgcgt gcggttcgtc gtccccacct ccacgagccg cgcgcccgac tgcgccacca
     2821 cgtcgggcac ccggaacgac ccgccgatct ccacgagctg cccgcgcgag accacgagct
     2881 cgcgccccgc cgcgagcgcc gcggcggcga ggagcacggc ggcggcgcag ttgttcacga
     2941 cgatcgcggc ctcggcgccg gtcagttcgc gcaggatcgc ctcggcgtgc gactggcgcg
     3001 acccgcgcgc tcccgcgtcg aggtcgtact cgaggttcga gtagccgcgc ccggcctcgg
     3061 cggcagcggc cgcggcggcc tcggcgagag gcgcgcgccc gaggttcgtg tggacgatca
     3121 cgccggtggc gttgaggacc ggccgcagcg tgccgcgcag cgtcgcctcg acccgagcgc
     3181 gcgcgtccgc gaggaggtcg ccggcggcgg ccgcgccgtc cgccgagccc gcgccgcccg
     3241 ccggggctgc gccctccgcc ggagccgcgc cgcccgccgg agccacgccg tccgccggag
     3301 ccacgccgtc cgcgatcgcc tcccgccgcg ccgcgatcgc ctcccgcgcc gcccgcacgg
     3361 ccacgccgtg cggcacgccg tcgagccgcg cggccagctc ctcgaccgac gggagggacc
     3421 tcagcgcctc acgcgccatc gggtcgagct ccctcgaccc agcgggcttc tccaccctcc
     3481 acctccctct tccagatcgg cgcctcggcc ttcacccgat cgatgatctc gcgcgcgccc
     3541 gcgaacgcct cgccgcggtg cgcggccgag accgccacga tcacgctggg ctcgccgagc
     3601 gcgaccgagc cgacccggtg ctcggccgcc gcggcgcaca gcccgtggcg ctcgatcgcc
     3661 tcggccacga tcgcctcgat ccgtccgacc gccatctcgg cgtaggcctc gtagtcgagc
     3721 cgctcgacgt cccgcgtcac gccctggaag gtgaccaccg caccggcccg gggatcgcgc
     3781 acgcgggcgg cgagcgcgtc gagcgacagc ggctcggcgg tcacgagcgc atgggccacg
     3841 gcggcctcgc cgcccgacac cggtgggatc agcgccagtt cgtcgccggg atcgagcggc
     3901 gcgtcctcgc gcgcgtactc gcggttcacc gccatgacca gcgggagccc gtcggccagc
     3961 tcgccgagcg ccgccagcgc gtcgcccacg cgcgcgccgt cgggcagctc cagcgtcagc
     4021 tcgctcgacc ccgcgcgctc gcgcagcatg gcgaagagcc ggaccgtgac ctccatgacg
     4081 caagcgtacg tccgtaggct ccgcgcgatg gccctcgcat accgcgttgc actgctcgac
     4141 tggctcgcgt gtgccgtcgg cggagccggc gagcccgcca cccgcgccgc gctcggggcg
     4201 gccgaggggc tcgagggccg cgtcgccgcc gcgggcacgg ccggccacgt gctcgacttc
     4261 gacgacacct acacgcccgg cctcgcccac ctgagcgcac cggtcgcacc cgccgcggtc
     4321 gtactcggcg gcgagctcgg cgcgaccgtg ggcgaggtcg tctccgccta cgcgaccggc
     4381 ttcgaggcga tggcgcagct cacgcgcgcg aaccatcccg gcatgcgcga gcgcggctgg
     4441 catccgacgg cgacgtgcgg agcggtcggc gcggccgccg cggccgccga gctgctcgag
     4501 ctggacgacg ggcgccggct cgcggcgctg cgcctcgccc tgctcgcgac gagcggcctg
     4561 cgcgccgcgt tcggctcgga cggaaagtcg ctccaggtcg gctccgccgc ggccgccggc
     4621 gtgcgcgcgg cgcggctggc cgccgcggga gcgagcgtcg gcgcgagcgc cgagcgcggc
     4681 tgggagcagg cctacggcgc ccgctgggtc gagccggagg cggcggccgg cgcgatccac
     4741 gcgaactgga tcaaggccca cccctgctgc ctccagaccc acggagcgat cgacgccgcg
     4801 ctcgcgagcg gagcctcgcc cggcgacggc gtcgaggtca cggtccaccc ggtgtccctc
     4861 acggcggccg agatcgacga ccccgccgac ggcctccagg caaagttctc gatcccctac
     4921 ctgaccgcct acgcgctcat gcgcggtgcg cccaccgtgg agagcttccg gtcgatcgac
     4981 gatgcggtcc gcgtcgaggc ccggtccatc cgcgtcagca cggacgcgag cctgctcgag
     5041 tccgaggcgg tcctcagcgc gcgcggcgcg gtcctcgcgc gcgtggaggc cgcggtcggc
     5101 tcgcccgaga acccgatgac cgccgagcgc ctcgcggcga aggtcggcca gctcgccggc
     5161 gggcgcctcg acggcgcact cgacgacccc tcccgagacg ccgctgaact cgctcgtatc
     5221 cttcccggcg gtggccacca cccctgagca caagccctcg atcgccccga tagtcggccc
     5281 ggacgacccg cgccggttca ccgactccgg catcgagatc aagacgctgt acgacgagac
     5341 cgacgtcgcg cccggcctcg aggagcgcct cggcaagccc ggcgagtacc ccttcacgcg
     5401 cgggatccac ccggacatgt accgcaagcg cacctggacg atgcgccagt acgcgggcta
     5461 cgccaccgcg gccgagacca acgagcgctt ccggtacctg atcgagcacg ggtccaccgg
     5521 gctgtcgatg gccttcgacc tccccaccca gctcggccgc gactccgacg acccgctctg
     5581 cgagggcgag gtcggccgca ccggcgtcgc gatcgacacg atcgacgaca tgcgccgcgt
     5641 gttcgaccag atcccgctcg acgaggtctc gacctcgatg acgatcaacg cgccggcggc
     5701 ggtcctgctg ctcctctacg agctggtcgg cgaggagcag ggcgtgcccg ccgagcagct
     5761 tcgcggcacc acccagaacg acatcctcaa ggagtacatc gcgcgcggga acttcatcta
     5821 cccgccggag ccggcgatgc gcctcaccac ggacctgttc gcctactgcg acgagcacat
     5881 cccgaagtgg aacacgatct cgatctccgg ctaccacatg cgcgagaagg gctgctccgc
     5941 cgtgcaggag gtcgcgttca ccctggccaa cgcgatcgcc tacgtgcagg cggcgatcga
     6001 cgcggggctc gaggtggacc gcttcggccc gcggctcgcg ttcttcttca acggccacaa
     6061 caacgtgttc caggaggtgg ccaagttccg cgccgtgcgc cgcatgtggg cgcagatcat
     6121 gcgcgagcgg ttcggctccg agaaccccaa gtcgcagacg atccgcttcc acacgcagac
     6181 cggcggcgtc acgctcgccg cgcagcagcc ggagaacaac atcgtgcgcg tggcgctcca
     6241 ggggttcgcg gcggtgtgcg gcggcacgca gtcgctgcac accaacggct acgacgaggc
     6301 cctggcgctc ccgaccgagc gcgccgcgcg catcgcgctg cgcacgcagc agatcctcgc
     6361 gcacgagtcc ggcgccaccg acgcggtcga cccgttcgcc ggctcgtact tcgtcgagtc
     6421 gctgaccgac gagatccagg agcgcgccaa ggagctgatg agcaaggtcg aggagctcgg
     6481 cggctcggtt aacgcgatcc agttcgtgcg cgccgaggtg gaggagtcgg cctggggcta
     6541 cgaccagcgc tacaagaacg gccaggacat cgtcgtcggc gtgaacaagt acgtgaccga
     6601 cgacgtggac gacgtcgaga tcctgcgcgt cgaccccaag accgaggccg agcaggtcgg
     6661 ccggctcacg gccttcaagg aggaccgcaa ccaggaggcc gtgcagaagc ggctcgagga
     6721 ggtgcgcgcc accgcccgcg gcgacgccaa cctgctggtg ccgatccgcg ctgcgctgaa
     6781 ggagcgcgcg tcgatcgggg aggtctgcgg ggtgctacgc gaggagttcg gcgagtacca
     6841 cgaggcgtga gctcgcggat cgccgcggtc accgccacga tccttgctgc cgccgccccg
     6901 cccgcgtccg cgccggaggc ggagccgcag gctcagccgg cactcacacc cgcgcagctc
     6961 gccggccagc gcatcgtctt cgggtacaac ggcaggggcg ggcgttgtct tcgagcccag
     7021 cgcgggtcac tcgactggcg ggcgcattcg cggcggggct gcgcgagggc ggcgtggccg
     7081 cgtgcgccaa gcacttcccc gggctgggcg gcgcgcggca cgaccaggac aagcgcaaca
     7141 accgggtcgg gttgagcgcg cggcgcctgc gcgccgagga ctacgcgccc taccgcacgc
     7201 tcgcgcggcg gggcatcgag ctggtgatga cgagcacggc cgactacccg gcgctcgacc
     7261 ggagccgccc cgcggtgttc agtccgcgga tcctggtgga cgagctgcgc gaacgtgtcg
     7321 ggttccgcgg cgtgacggtg acggactcgc tcggggtgga gtccgcgagt cggctcggct
     7381 cgccggaggc gcgtgcggtg cgtgcggcgc gcgccggcaa cgacctcctg ctgttcgcgt
     7441 cgagctacgc ggcgggggtg cgtggcgcgg aggcgctggc gaagcgcgcg ccgagcgacg
     7501 aggcgctcga ccgcatcgag gcgctgcggc gggcaattcg ctgagccgcg gctgccgccg
     7561 cgcgcgaccc ctcgcccctg ctaccttcgc gccagtgagg agggggaccg cagtccacgt
     7621 gcacgtggcg ctactcgcgc tctgcgcgct cctgattgtg gccgcgcccg ccgcggcggc
     7681 cggcaagccg aaggtccgcg ccgagatcaa gcgcacggcc tacggcatcc cgcacatcaa
     7741 ggcgtcgaac tacgagggca tcgcgttcgg ctacgcctac gcgctcgcgc aggacaacat
     7801 ctgcgagctc gccgacatct acaccaccgt ccgcgcggag cgctcgcgct acttcgaccc
     7861 gcaggccacc tacgcctcgc gcggcaacgg cgcgaccttc aagaacatcg actccgactt
     7921 cttcttccag cgcgcgatcg acacgaagat cgtcgagggc ctgatcgcca agccgccgcc
     7981 ggagggtccg ctgccgatcc tcaaggaagc agttcgcggc tacgcggcgg gctacaacaa
     8041 gtacctgaag cgcatcggtg gcccgaacgg cctcgacgac ccgcgctgca agggcaagga
     8101 ctgggtcaag ccgatcaccg agatggacgt ctaccgccgc ttctggcagc tcggaatcct
     8161 ggcgagccag ggcgtcgcga tcgacggcat cgccacagcc gcgccgctgg cgccgggtgt
     8221 cccgctgctc ggcggcgcag gcgcggccgc gaacccggcg atgctcgacg agctcggcag
     8281 ccgcctcggc gaggagctcg gcatcggctc gaacgcggtc ggcgtcggct cggagggcac
     8341 ccgctcaggc ggcggccttc tgctcggcaa cccgcacttc ccgtgggacg gctccgagcg
     8401 cttctaccag acccacctca cgatccccgg caagctcaac gtcaccggcg gcagcctgct
     8461 cggcgtcccg atcgtgctga tcggccacac cgacaagatg gcctggagcc acaccgtctc
     8521 caccgcgcgg cgcttcctga tctacgagct gaagctcgtc ccgggctcgc ccacgagcta
     8581 catccacgac ggccaggtca agcagatgaa ggccgacaac gtgacgattc aggtgcgcaa
     8641 ccccgagggc gggaccacgc cgcagacgcg tacgctctac tcctcagaac acggctcgat
     8701 cctcacgagc atcctcgggc tgccggtgtt cccctggacg gccgagcgcg cgtacgcgat
     8761 ggacgacgcc aacgcctcga acttccgcta cctcaaccac ttcttcgagg tcaaccacgc
     8821 gcagagcgtg cgccaggtcc acgcgatcga gaaccgctac cagggcatcc cctgggtcaa
     8881 cacgatcgcg gcggacaaga agggcgaggc ctactacgcg gacatcggct cgatgccgaa
     8941 cctcccgaac gagaagctgt ccgcctgcga ggtgcccgtc ggcaccgcgt tgcgtaccgc
     9001 cgcaggcgtg ttcgcgatcg acggctcgcg cggcgagtgc gatcccgggc aggacccgac
     9061 cgcggtcgct cccggcatcc tcggccccga caagcagccg ttcctgttcc gccgcgacta
     9121 cgtggagaac tcgaacgaca gctactggct caccaacccc aagcagccgc tcgagggcta
     9181 cccgacgctg atcgggcccg agcgcaccgc gcgcgccctg cgcacccggc tgggcctgcg
     9241 gatcatccag cagcgcctcg acggcaccga cggccgcccc ggcaccaagt tcgggctcgc
     9301 cgacatgacc tgggcggtct tcaacaaccg ccagtacgcg ggcgagctgt ggcgcgacga
     9361 ggccgtggcg atgtgcaacg ccaacgcctc gagcatgccc agctcgaagg ggccggtcaa
     9421 cgttgccgac gcctgcccgg tgctcgcggc gtggaacgtg cgcgacgacc tcgactcgaa
     9481 gggcgcgatc ctcgcgcggc gcttcatcaa ccgcgcccgg gccgttcagg gcgggccgta
     9541 ccgccgcgcc ttcgaccccg ccgatccggt caacaccccg agcggcctca acaccgagaa
     9601 caccgccgta cagcaggcgt tcggcgacgc ggtcaacgac ctgcgcgacg ccggcatccc
     9661 gctcgacgcg cccctgcgcg ggtaccagta cgagatgcgt ggcgacaagg cgttcccgat
     9721 ccacggcggg ccgggcaccg acggcgtctt caacgcgatc aacgtgagct ggtcgccgcc
     9781 gaagggctac ccgaacatcc cgcacggctc gagcttcgtg caggtggtgc acttcccgag
     9841 gaagggctgc gccgagcacc gcacgatcct cacctactcg cagtcgacca acccgaactc
     9901 gaagcacttc tccgaccaga ccagcctgtt ctcgaagaag cgctgggtta accccccgtt
     9961 ttgcgcgtcg cagctgcgca aggcgaaggg aagtacgcag cggctggcgc gctgacgcgc
    10021 cttctctaga ggccgcgcag cttgaccagg gtggtcgcgg gctcgtagcg ctcgtcgccc
    10081 gtgtagcgga ggctcagggt gccccgcagc cgctgtggcg cgcgcagggc gagcttgaac
    10141 cgtcccgctt gcgggaacgc cgcgccgttg cggcgcactc gcttcgtgcc ggacttcgcg
    10201 ttcagctcga cggtcagccg ccggaacgcg tcgctcgcga tggtgcccct gagctcgatc
    10261 cgcgaccctc ggcgagcgat cgaccggacg cgcagccgcg ggttcgtccg gatcgcgggg
    10321 cccggaggcc gcggaaccgg ctgtggctcg gcacgcggtg cctcgaccac gacctcgtcg
    10381 gtggtggcgt acccggaggt ggtgccgaga cggtcggtgg cgcgcacgcg gaagcggtag
    10441 cgagcgccgg gtgtgcccgt gtaggtggcc gcggtcgcgg aggtggcggt cagccacggt
    10501 gagaaggcgc cgtcgttcgt ggcgacctcg acggtgtagt cgcgcagtcc cgcccccgcg
    10561 tccgtaccag accaggcgat cgggatcgcc gggtcggtga cgccgcgcgc cgggacggcg
    10621 atcgcgacgc tcggaggcga cccgtccgag gtgaacgtgg cctggtcttc ggatccgaac
    10681 gtctcgccat agcgcgatgc ggtgctgcgc acggcgatcc tgttcacggc gtcgccgctg
    10741 gcgcggacgg tccacgacac aaccgtgctg gcgcccggcg cgagcgtgcc gaccggctgg
    10801 gtcgcggacc cctcgtcgat cgcgacgccg ggcggcaggg cgatcgtcgc gctggcggat
    10861 tccgcgctga ggtcgggtga ggggttcgac agctccgcca tcacggtgac gggctcgccg
    10921 gggcgcgcga ccgtcgtgga cagagtcacg cgcgcacgtg ggcggggtgc cgcgagcggg
    10981 gtgatctggc gacgcgaggc gagcgcgtag ggctcgccgg gcaggccgtc aacggtcgag
    11041 gaggccttca ccttgtagat cacggggccg gtggcgggcg agcgcacctg ctcgacgttg
    11101 tcgatgccgc tggcgctgcg ggcctcgacc gcgccggtgg ccggatcgag ctgctcgagc
    11161 tccaggttcg agagggtgag cgtcgtggtg tcgcaaccgg ggtcgatgca gccgagcgcg
    11221 cgcctgttcc atacgagcgt cgcccgatcg cccgcggcct ggacgtcggc gcggtagaag
    11281 tgagcgtcgc ccccagccac gtcgtccatg tagaagtggg tgcgctcctt gacggccgcg
    11341 tcgaggtcca gggcgcccca accccagtcg ggctgccagc cggcctgagt acccatcggg
    11401 ctggtcgggg tggcgcggcc ctgccgggcg gagttgatca ggatggcctt ctgagcgatc
    11461 ggagcggcga tgcctgagcc ggcgagcaga gcggccgcgc cggcaccctg cggggccgcg
    11521 agcgaggtac cgctgccgcc actccagagc cgcccgtccc ggatccagtg ctgattggcg
    11581 aactcgctgg tggacacggc aacgagatcc ggcttcttcc gaccacccgg cgtgggcccc
    11641 cggctggaga aatcctcgac gacgtcgtcg gcggggtcga cggtgccccg atgatcaaat
    11701 cctcccacgc aaagcgtgtc ataggcgatg cagtttgcgt tgacggtgcg cgcaggaccc
    11761 tcgttgccgg cggggtaagc gatcaatgca ccgtacgcag agatgagctt gtcggtacgt
    11821 cgcagtgaac ctgagtcatc agcctcagca acagaaccgt ggctattact cattacttcg
    11881 gctgggtcgg tcgcaccttg gattccgaac gacccaacgc cgagggccca tgcggtgtca
    11941 tcggccatac cagatgcctg tgcgtcgagg accgagtcca gacgcggcgc tacgcccatc
    12001 ctcgaagagt cgctgctcac gcacgccgcg caccccgcag cgccgcgact gatcgccatg
    12061 ctcgcgacct cggtcccatg ttcgcagccg ctcacccgtt gtccacaact cgttccgatt
    12121 ggcgcgtctg aaggacgttg aaagtggatg ccagcgaagg ctgggtgctc ctgctcaatc
    12181 ttgtcgtgaa ggatggctag gtcgaccgga taggtatcgc tagcgccacg tccgcccaga
    12241 tgtccggcgt cccacatggc tggtgcgccg atcgccgctg tggatagctg aagacttagg
    12301 tcggcctcga cgtgtacgag ctcgactgat ctcacattcg gcagcgcgcc aacagccgca
    12361 agcgactgtg ccggcagcag cgcagtgaca gatccggtag ccagatcgtg ctcgaggatt
    12421 atggctccga catcgcgcat cgccgcagcc acgtgatctt ggtccgcggc ggcagcacga
    12481 gcggcggcgc ggacgtcaac gagtccacgg cggcgccgtt cctcgaggtg gcttgcaagc
    12541 gaccccaaat gcctacctgt ccggaggacc gccacctgac gctcgttgag gtggccagtt
    12601 atcgctacac ggttggtcgc agcgtcagcg cgacgcagca tcgggctgtg cctcagtgtg
    12661 acgagggccc gaacccgccc actatgcggt ctgctcgcgc ctccatctga agcagcaaat
    12721 gcctgggccg gggggagatc cgctcccaca ccaccaccac ggcggtcagc ggttgcgtcg
    12781 cgtggaacga cccaagcaca ggttgccgca gcgactacga ctacaaggat tcgacgaaac
    12841 atcccagcca cgcgctcaga atcggcttag cggccgacac ggtttacccg tgccgaccgc
    12901 ctctcgccga atgtgcccta tgggccgcgt acgacgacac gcacggtttc ggagtaaccg
    12961 cggtcgtatg ggtacgcggc ttcgggtcgg atcaggaccc ggaaccgata gggcaccgtc
    13021 ccgcgcgtag ccccgaagcg gtagcggtag ctccacttcg ccttggcatt cgcgcgtgga
    13081 gtcgcaaacg ttcgccactt gccgcggtag aacgcttgta gatccagcac ctttcctgcc
    13141 gacgggatcg gcagcgagcg cagcttgccc cggaacgtga cgctctggcc gttgcgtcgc
    13201 gtccgatggc tggcggcgat cgtcgctgca gcggcgacct tgagggtcac ggctccgtcg
    13261 ctcgaccgat gcttcgagtc gccgtcgaat gcgaatcgga tcgcgcggct ggtaccggcc
    13321 cgcgcgacgt actcgaacgc acccttcggg tctgttcgga cagcacccac gcgctcgaac
    13381 tcgctccccg tagccgccga ctgtgcatac acgtcgatca cggcgttgcc caggggcgca
    13441 cccgtgtctg actcgagcgt gccgcgagcg acagccccct tcccgaaggg gacactcagg
    13501 gttccgacca gttcgccgcc gggattcttc gtcgccttct tccggcactt cctcttcttc
    13561 ccggggcgct tcttcgagca gaccgtcttg gtcttcttgg tcgattgctt caccaacgcg
    13621 gcggacagct tgctcccgct acgcagagcc gcggtgtcga ccgtggccga cgagccgtcg
    13681 cgacggcgat cgccggcgga ctcgttgccg gcctggtcct tcaccctggc ccgcagctga
    13741 tagacgccgc gttccaccga ctcgtcatcg acggttgcca cgaagcgatc cccgtcttga
    13801 gtagtcggca actcgatcca gcggccgtcg ccgtggaggc gctgcatctc gatcgtcgca
    13861 cttgcgacgc cggaggtgcg gtcgctcgcg gcgaccacga cgcgccgcgg gtctgcggcg
    13921 tcgggtgcct cgaacaccac gagctccggc gccgtgcggt cgatcttgaa gctgaccgct
    13981 cgctcgatcg acgcattgcc cgcgacgtca aacgctctgt aggtcagggt gtgggcacca
    14041 tcgtcggcga aagccagacg ccccacgccc cctcggactg tctgccggac agacccatcc
    14101 agcctgtatg cgatgtacgc gccatcctct atgggaaggt gctcacgagc aggctcgagg
    14161 cccgagaggt gatcctgatc tgacgctgta atggagacat tgactggctc acgatgccag
    14221 ccgtcagctc gaggatccag cgcagcgctt actgtcggaa tcgacttgtc gacacgtatt
    14281 tcgctgcgcc caaccgcggc agacgccacc cccgaacctg agactgccct cgctttgaag
    14341 acggtcgttc cctcgggaag cgcatcaatc cggtatgtct cgcctacagc atcgaggaca
    14401 gagtcaggct cgcttccgtc cgtggtcacc gaataaccga caacacccga cactggttgg
    14461 aaggcgccgt ccaccatcct gatgtcttgc tcgtagttcg cggcttcatc ggcgtttagc
    14521 cagccattcc gctttgccgg cgccgcctcc cccggcgcca cgttgtcgaa ccgcaaactc
    14581 acctcgtccg accagttcgc ctccttcacg ttgcccgcct ggtcctccag ccagagccgc
    14641 agcgtgtagt gacccggagc gggcaccgag aggtcggtga tcgagttgat cccgccgccc
    14701 tggcgagccc cgaccgtgca ctgctgcgtg gccgtgctgc acaggcggta gcgcgcgatc
    14761 gttatcggcg atgcgctcgc cgggttctgc catgtcagct tgaactggtt ggtctgccgc
    14821 cagccgtcgc cgccctcgac cctcagctcg cgcggagcat ccggcgccgt gttgtcgctg
    14881 tggaactcgc ggtccatccc gccgacattc ccggccgcat cgacgccctc gacccgcacg
    14941 acatgccggc cgtcgggcag ccccgcggtg ttgacgtcgt agaggtcggg cggcacgtcg
    15001 agacacgggc gcgctcgcgt gaagtcgcag ccgccgcgct cgtcctcgcg cagacggttc
    15061 ccgtccacgt agagccgcgt tcgacgcaac ccgacgttgt cgctgatccg gtacccgacc
    15121 gcgttggtcc ctcgctgcca ggccccgttg tcgagcctcc cctgcccggt gtccaccacg
    15181 ctcggcgccg agtcgtcgtg cacccgcacg gtcgccccgt acaggcgcac accggcgcgg
    15241 tcgccgtgcg gcgccgccgc gtcgctgcgg cagcccgtcc cacttccgca cgccgtcacg
    15301 accgacacct ggtggatctg gcccggggac catccccacg tcgag
//
LOCUS       DASVPL010000025         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_80994, whole genome shotgun sequence.
ACCESSION   DASVPL010000025 DASVPL010000000
VERSION     DASVPL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80994"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VF517_01315"
     CDS             complement(<1..490)
                     /locus_tag="VF517_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051601.1"
                     /translation="MTAPPLRVLLGVGLIAAATLALQVLLTRVLSAVLFYHFGFLAIS
                     LALLGIGAGALVIYVRPALVEGRSVERLLAFSSAAFALLMPVVAALLVRIDYTYTEVT
                     AGFVARLALACSLAVLPFLAAGVTLAVAIRSYTPWIGRVYAFDLAGAALGAAGVVPLL
                     WSV"
     gene            complement(487..1698)
                     /locus_tag="VF517_01320"
     CDS             complement(487..1698)
                     /locus_tag="VF517_01320"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8051602.1"
                     /translation="MTAPRNPRFPLFDSLRAIAALSVLLFHIAFVLEGFTDPNWGRYA
                     TQLNIGVTIFFLISGFLLYRPFARARYAREPLPGIRAYATRRLFRIVPAYWVALAGIA
                     LWVDLPVVFEHPWFHFTFTHVYNEEDLLTGVGHTWTLAVEMTFYIFLPVWAFLLRRLP
                     RGGSDRQFVLSEALPLVAIYAVGLIWNLTQVKHVNGLVLFSPEVAALPRFLDHFALGM
                     GLAVASVVIAGRERPPRLVRWVEERPWVPWALAGAGFVVLANLGSSYISAGVEPVRHE
                     LRGLIALCVLLPAVFGEARGGAVRRLLAWPPLVWVGLVSYSLYLWHPAIAQKIIYTDL
                     DESVGWLLPALLTVIASIAVAAVSFYLIERPALRLGRRLTSGGRHETPGDPGAEGGLG
                     SPADEQMAART"
     gene            complement(1695..3269)
                     /locus_tag="VF517_01325"
     CDS             complement(1695..3269)
                     /locus_tag="VF517_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051603.1"
                     /translation="MTRPRLDRFELLLLAALFALSVAVLVGLLLRVWTKGGVITGSDG
                     FLVVDQMQYLNWLRQAGEHVTVGNLYDLDPGGPRSFLHPGVLVSGLLHRLGLGVAAAY
                     MAWKPVAVIALFAAALAWTRRFFTRRDDRRLALALVLFTASPVAALVGWAGLGDPHDK
                     LSFDFLSNEMWSGNWLWGYLFTAIAVAMLPLALIAYERGRGSPSPRWLLAAGAAGMVC
                     AWFQPWQGATLAIVLVAGEALARRHDATPLGRSIRDLALPLALTAAPLVYYLVLSHTD
                     PAWELAGSSNDTETQPRWPVWVTIVGLAPLAVPALLAYRIPARDFAQIALRAWPLAAL
                     AVFYQPAGTFPFHALQGMAFPLAVLALLAVRERLGERPLPVLPALAVAAILIVPGTLY
                     NGDQMRDAINRGFQAHFMEPGERAALRHLDDEREEGGVLAPAYLGTAVPAYTGREVWA
                     GAGSWTPDFVERSDQANRLFGGRLPRPAAEALVRRSGARFLFSDCHDRADVTALLARV
                     TEPPRRFGCATVWRVR"
BASE COUNT          560 a         1192 c         1328 g          332 t
ORIGIN      
        1 tcaccgacca gagcagcggc acgacgccag ccgcgccgag cgccgcaccc gcgaggtcga
       61 acgcgtagac gcggccgatc cacggcgtgt acgagcggat cgcgaccgcg agcgtgaccc
      121 ccgcggcgag gaacgggagc accgccagcg agcaggccag cgcgaggcgt gcgacgaacc
      181 cggccgtgac ctccgtgtac gtgtagtcga tgcgaaccag cagcgccgcg acgaccggca
      241 tgagcagcgc gaacgcggcc gagctgaacg ccaggagccg ctcgaccgag cgcccctcga
      301 ccagcgccgg gcgcacgtag atgacgagcg cgccggcgcc gatgccgagc agcgccagcg
      361 agatcgccag gaagccgaag tggtagaaga ggaccgcgga gagcacgcgc gtgaggagca
      421 cctggagcgc gagcgtggcg gccgcgatca ggccgacgcc gaggagcacg cggagcgggg
      481 gcgccgtcac gtgcgcgccg ccatctgctc gtcggccggc gagccgagcc cgccctcggc
      541 gcccgggtcg cccggtgtct cgtgccggcc gccgctcgtg agccgccgcc cgagccgcag
      601 cgccgggcgc tcgatcaggt agaagctcac cgccgcaacc gcgatcgacg cgatcacggt
      661 caggagggcc ggcagcagcc agccgaccga ctcgtcgagg tccgtgtaga tgatcttctg
      721 cgcgatcgcc gggtgccaga ggtagagcga gtacgagacg agccccaccc agacgagcgg
      781 cggccaggcc agcaggcgcc gcacggcgcc cccgcgcgcc tcaccgaaca ccgcgggcag
      841 cagcacgcag agcgcgatca ggccgcgcag ctcgtggcga accggctcga ccccggccga
      901 gatgtagctg ctgcccaggt tggccagcac cacgaagccg gcgcccgcca gcgcccacgg
      961 cacccacggg cgctcctcga cccaacgtac gaggcggggc gggcgctcgc gtccggctat
     1021 caccacgctc gccacggcca gccccatccc gagcgcgaag tggtcgagga agcgcggcag
     1081 cgcggccacc tccggactga acagcacgag cccgttgacg tgcttgacct gcgtcaggtt
     1141 ccagatcagt cccaccgcgt agatcgcgac gagcggcagc gcctcgctca gcacgaactg
     1201 ccggtcgctc cctccgcgcg gcagtcgccg cagcaggaag gcccacaccg gcaggaagat
     1261 gtagaaggtc atctcgaccg cgagcgtcca cgtgtgcccg acgcccgtga gcaggtcctc
     1321 ctcgttgtag acgtgcgtga acgtgaagtg gaaccacggg tgctcgaaca ccaccggcag
     1381 gtccacccag agcgcgatgc cggcgagggc gacccagtac gcgggcacga tccggaacag
     1441 ccgccgcgtg gcgtacgcgc ggatgcccgg cagcggctcc cgcgcgtagc gggcgcgcgc
     1501 gaacgggcgg tagagcagga accccgagat caggaagaag atcgtcacgc cgatgttgag
     1561 ctgggtggcg tagcgccccc agttcgggtc ggtgaagccc tcgagcacga acgcgatgtg
     1621 gaagagcagg accgacagcg ccgcgatcgc gcgcagcgag tcgaacaggg ggaagcgggg
     1681 gttgcgcggg gcggtcatcg gacccgccag acggtggcac agccgaaccg gcgcggcggc
     1741 tcggtgacgc gtgcgagcag ggcggtcacg tcggcgcggt cgtggcagtc ggagaacagg
     1801 aagcgcgcgc ccgagcggcg cacgagcgcc tcggccgccg ggcgggggag ccggccgccg
     1861 aagagccggt tggcctggtc gctgcgctcg acgaagtctg gcgtccacga gccggcgccg
     1921 gcccagacct cgcggccggt gtacgcgggc acggccgttc cgaggtaggc gggagcgagc
     1981 acgccgccct cctcgcgctc gtcgtcgagg tggcgcagcg cggcgcgctc gcccggctcc
     2041 atgaagtggg cctggaagcc ccggttgatc gcgtcgcgca tctggtcgcc gttgtagagc
     2101 gtgccgggga cgatgaggat cgcggccacc gccagggcgg ggaggacggg cagcgggcgc
     2161 tcgccgagtc gctcgcggac ggcgagcagc gcgagcaccg ccagcgggaa cgccatcccc
     2221 tggagcgcgt ggaacgggaa ggttccggcc ggctggtaga agacggcgag cgccgcgagc
     2281 ggccacgcgc gcagcgcgat ctgcgcgaag tcgcgcgccg ggatccggta cgcgagcagg
     2341 gccgggaccg cgagcggtgc gagcccgacg atcgtgaccc agacgggcca gcgcggctgc
     2401 gtctcggtgt cgttgctcga tcccgcgagc tcccacgcgg ggtcggtgtg ggagaggacg
     2461 aggtagtaga cgagtggcgc ggccgtgagg gccaggggga gagccagatc gcggatcgag
     2521 cggccgagcg gggtggcgtc gtggcggcgg gcgagggcct cgccggcgac gaggacgatg
     2581 gcgagcgtcg cgccctgcca gggctggaac caggcacaca ccatgcccgc ggcgccggcg
     2641 gcgaggagcc agcgggggga cggcgagccg cggccgcgct cgtaggcgat gagcgcgagc
     2701 ggcagcatcg caaccgcgat ggccgtgaac aggtagcccc agagccagtt ccccgaccac
     2761 atctcgttgc tgaggaagtc gaagctgagc ttgtcgtgcg ggtcgccgag gccggcccag
     2821 ccgaccaggg cggcgacggg ggaggcggtg aagagcacga gcgcgagcgc cagacggcga
     2881 tcgtcgcggc gggtgaagaa ccgccgcgtc caggcgagcg ccgcggcgaa cagcgcgatc
     2941 accgcgaccg gcttccaggc catgtacgcc gccgccacgc cgagcccgag ccggtgcagc
     3001 agccccgaga ccagcacgcc ggggtgcagg aagctgcgcg gcccgccggg gtcgaggtcg
     3061 tagaggttcc ctacggtcac gtgctcgccc gcctggcgca gccagttcag gtactgcatc
     3121 tggtcgacga cgaggaagcc gtcggatccc gtgatcacgc cgcccttggt ccacacgcgc
     3181 agcagcagac cgacgaggac ggcgaccgac agggcgaaca gcgccgcgag caggagcagc
     3241 tcgaagcggt cgagccgcgg gcgcgtcaag accgcggttc ggcgggctcc gccacgagcg
     3301 cgtcgcgcgc ggcggcgccc ggtgcggagg cgcccggcgc gggggcgccg gaatcgctcg
     3361 gcgcgggctc gctcggcgcg gggcttgcgc ccgccgcggg gcttcggccc cc
//
LOCUS       DASVPL010000026        14596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_355097, whole genome shotgun sequence.
ACCESSION   DASVPL010000026 DASVPL010000000
VERSION     DASVPL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14596
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355097"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /locus_tag="VF517_01330"
     CDS             complement(<1..1882)
                     /locus_tag="VF517_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051604.1"
                     /translation="MSPRPARRLDRLRSRRRAIVGAVLAGLVAALVTGAPSSAEDACT
                     IAWDGGAGSDVWREAANWTGNRLPGPADTACIPGGTSVRYFFGSDTVTALGGEGSVTV
                     FNGLLTTTGSSSIGGLTLGSSGTARIDGELSVATMVQSGGNLLGSGRVSAGSLTWSAG
                     GQGGTGTTVVREGATLAGGIRSLFEARRFEVARGATATWTAGDIEIRDTARLANAGVL
                     EVRGDQDFTSSGFNSAAQILNEASGTIRKASGSGETVTFKLANAGAVEVLAGTVSVLS
                     GESTGSIHAGAGATVRFAVGGSTLAPGSTTTSSGDGRVLFAGGVVHAAGAYTARTVVN
                     GAGTLWFDAAVSIPRLELRNGNVSGAGTITTPDVAWSNGSLVGDGSTTIVPGGPGVVA
                     SGAGERRLERRTLAIEPGAGLRVADGSLMLADRARLENAGRVEVVDAARIELCCDQTP
                     VVHNLTGGVVAKTGAGRGEITPSFVNDGTVAADEGTLAFSGLLTNLRGRVLSGGTWRV
                     RAALELRDRIDRNAARLTLDGPASSVVDGFARADALRELTANDRAGELTLDNGRALAV
                     ATFENSGGVTLGRDSSLSVGARYVQHDGTTRLTSAGSRLSSGGGAADIRGGVLSGIGT
                     VAG"
     gene            complement(2168..5659)
                     /locus_tag="VF517_01335"
     CDS             complement(2168..5659)
                     /locus_tag="VF517_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX8051605.1"
                     /translation="MLAVAVALLAVPAGASAAQILAGVAVKDATWHVGASAGQYASGG
                     DLNENDAGGAFASVHSSDPHQHSTRRAPSYGVQSRLSARAIVVQGANGSKFALVKNDL
                     YIPQDLLWRRTAQILESKSIDIDRDNLTMTSSHNHSSPLYSSTGWGVWVFQDVFDFRY
                     FEYMAQTMASAVAEADRTKVPVKVGASVTQYDFVQRNVPGPSTADDGTPAGFPNDYTD
                     HDLMVVRFDRLDNGKPLANLVNYAVHPEDNEGNDLISADYLGPMERMTDRATGAVTIY
                     TQGAVGNTEPEDNRWHNVHERAFFSHAQYAQSEFKGRGIASNIVKTWKDIEAGTPENP
                     QRFVPFRSEFGADDVKFADKWFPGPASHPYPGISSCRTDSALAGDPRAPVVGFPDCAA
                     LSDYRQEYFDDNCRGNGPDEFCNGPPQAFEEIKGAGVTTDTIQVLGLPVPENYSAPAY
                     TGLEEDVSVHLQAFRIGDILMTVCSCEQWADQSKNIKSRTDRVAGNQHNGYDWTAQCV
                     RAGADWTCQHPNGQRTISDALYQKIRAQVNNDAKGWDDLTYVAQAESEPTDPALIKGN
                     YTHDDNAASAGLGYGLTLAIGMGNDYNGYIATYREYQRGDHYRKALTAWGPHSSDYMA
                     TRLVKMGRSLKPGQADPFPVVPGNLNVVSTVTPTGPLPGELPGGEAKTTADLQNNENR
                     AQVIGQFATGGMAAYEASLPNDLKAEPVKQPEDVERFGSSFFTWVGGSNYTDNPQVKV
                     QRFTGGNWTDWADQSGELPVTLKFPPSEDRPAYRVQGSRFEWTAHYEAFVSRYDLMDR
                     PVATPPGSYRFVVRGQRQQDGKPARYDLTSKEFVVKAWTGVTIEGFGADGGQPSFKVG
                     PRRTVKAFNIEARLGPIDYPDTWQSNYGSKIPFIKPDRRAYRDPAAPNDPDRFEWYCQ
                     PAQDAGDANKPHGCSFRPWLDVGDLERVVFTFVSKNGVVDRVSGKKVGGEWVADRKLK
                     SGEGVYIESGDACDAYGNYNGAPTKVAGNANAVPDKPPTGFSCVPRQPVGDTPGGSTA
                     GGGGGGGAGGGSGSAGGGSNPLGLPPKSKCLDRRRFKFKIHQPPRQRVVNVNVYINGK
                     RRYSRRGTKVTTVSIKKLPSTGTYVVRIVALTNRGNRVISTRRYKGCKKGRPTTRVDR
                     GGKKKTTKKTTAAPSRR"
     gene            complement(5747..7093)
                     /gene="radA"
                     /locus_tag="VF517_01340"
     CDS             complement(5747..7093)
                     /gene="radA"
                     /locus_tag="VF517_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX8051606.1"
                     /translation="MPRSVHVCSACAHESVKWHGQCPGCGEWNTLVEEVPAAAPAGRA
                     KRAAPALRPVALADVEAPRVPRLGTGIGELDRVLGGGLVPGSVVLLGGSPGIGKSTIT
                     ASALGNLAAAGRSVLYVSGEESAAQVKLRAERLGPDALRVPIVAETSLEPVVATLEAE
                     RPDVCVVDSVQTLQCADLSGTAGSVGQVREVAGRLARVAKERGVATVLVGHVTKEGAL
                     AGPRVLEHLVDCVLQFEGERERTYRTLRALKNRFGSTNETGVFEMRESGLVEVADASA
                     RFVEEATRAPGSVVLCAMEGSRPLLVEVQALVAPAQSEPPRRIANGIDRNRLSLVLAV
                     LARHGGVALGGSDVFVSVAGGVRVEEPGSDLAVALAVASAARGVRAAGDGLRPLAAFG
                     EIGLTGELRHVAHPDRRLAEAAKFGLADVVHPAGAVKTVRAALARAFDGAAEARAA"
     gene            7239..8435
                     /locus_tag="VF517_01345"
     CDS             7239..8435
                     /locus_tag="VF517_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX8051607.1"
                     /translation="MTPRRPFLALLAVAAAALGASPAAAAVATGPSGDAFYTPPKKLP
                     GKTHGDPIWIRKLTNQQTLAEAKVNESVLYRSTSVRGKTVAVSGTIHLPKGKAPRGGW
                     PVVSWAHATSGLGDQCAPSRNPGEGAIHAYHVYIEALLDRWLKAGFAVVRTDYEGLGT
                     KGVHPYLVGVSEGRGVLDIVRAARKVHPGLLSTRFAIGGHSQGGHAAVFATALARKWV
                     PELSLRGTTAFAPFSQGDEIFASLAQLDQPSRVSGYAALFVRGIEAGYPQLGVTSLLS
                     QRGADLYPLTLSVCIDEITSESSEFAKTAPRDLFREGADLEPIRTTLAANDPTDLKLT
                     TPLLVEQGSSDTTVNPNFTQAMVAGLRKSGAKVTLKLHENIDHTTVAFGEPQDEAFSW
                     VSKRLR"
     gene            complement(8436..8663)
                     /locus_tag="VF517_01350"
     CDS             complement(8436..8663)
                     /locus_tag="VF517_01350"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX8051608.1"
                     /translation="MPDPVEIKVRENGPYRVTGPITLVDADGNRFEIPDDGRPVALCR
                     CGGSTTKPFCDGTHSKIGFDAATRAVRAADE"
     gene            complement(9301..11934)
                     /locus_tag="VF517_01355"
     CDS             complement(9301..11934)
                     /locus_tag="VF517_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX8051609.1"
                     /translation="MFERFTERARQVVVLAQEEARILKHNYIGTEHILLGLLREEEGL
                     AARVLESLDITVERVRAQVVRIVGSGEEVTSGQIPFTPRAKKVLELALREALSLGHNY
                     IGTEHILLGLVRENEGVAARILLDFDADSEKIRNEVIRMLSGPGGRRQQGQGAQGQQG
                     EGKKSSKLLDQFGRNLTRLAAEAKLDPVVGRETEIERIMQILSRRTKNNPVLIGEPGV
                     GKTAVVEGLAQRITHGEVPELLKNKQIYTLDLAALVAGSKYRGEFEERLKKVMKEITQ
                     RGDIILFIDELHNLVGAGAAEGAIDAASILKPALARGELQTIGATTLDEYRKYLERDS
                     ALERRFQQIRVDQPSIEETEQILKGLRDRYEQHHRVDITDEALHAAAELADRYISDRF
                     LPDKAIDLIDEAASRMRIKSMTAPPVYRDLEEEIETTRRDKEAAIEAQEFEKAANLRD
                     QERQLTNKKRELEEQWRAGESGERPAIGEEEIADIVSMWTGIPVFKLTEAETQKLMRM
                     EDELHKRVIGQQAAIEAVSKAIRRSRAGLKDPKRPTGSFIFLGPSGVGKTELARTLAE
                     FLFGDEDAMVRIDMSEYMEKHSVSRLVGSPPGYIGYDEGGQLTEAVRRKPYSVLLLDE
                     IEKAHPDVFNVLLQILEDGRLTDAQGRTVDFRHAIVIMTSNVGASEIAKNTGVGFTIG
                     DDTGITYDDMKNRIMGELKKLFRPEFLNRIDEVIVFHKLTKEEITEIVELLLRRIRES
                     MAERELSLNLSDDARDLLVEKGWDPAMGARPLRRAIQRYIEDPLADEVLKADDMQPGA
                     TVEVDRAEGGDGEEPEVKISITAPKREPVTVGGDGEAEAGGDESQGSLPDTGSLPDVP
                     DVLPEAPDAPPSDEPGPPSES"
     gene            complement(12134..13597)
                     /gene="lysS"
                     /locus_tag="VF517_01360"
     CDS             complement(12134..13597)
                     /gene="lysS"
                     /locus_tag="VF517_01360"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX8051610.1"
                     /translation="MTEQPKTEAQALDDRRAKLERLRAEGIDPFPHAYPGAVAIESVH
                     GAHGELEAGQETEATYRVAGRLSARRGQGKAAFLDEVDRSGRIQVHARVDVLGPMEFE
                     RLLSLDLGDLVGIDGTAFKSRRGELSLRADGWTLLAKSLRPPPDKYHGLQDVETRYRQ
                     RELDLIANEDSRRLFLLRSQVVSRIRRHLDDRGFLEVETPVLQPLYGGAMARPFVTHH
                     NALDRDLYLRIATELYLKRLIVGGLERVYELGKDFRNEGISHKHNPEFTMLEWYEAYG
                     DYETGAERLEELVTDVVGASGAPGEGIDWQRPWARLKLRDAVLDATGVDVSAAEDTET
                     LVAAAKERGIELDPTESWPQLVDELLSKHVEPKLMNPTFVMDYPVALSPLAKRHRSED
                     GLVERWECFAGGIEIANAFSELNDPDDQRARFEEQQRLGAEGDEETQPYDEDYVRALE
                     HGMPPTSGVGLGIDRLVMLLAGRTTLREVILFPAMRN"
     gene            complement(13637..14113)
                     /gene="greA"
                     /locus_tag="VF517_01365"
     CDS             complement(13637..14113)
                     /gene="greA"
                     /locus_tag="VF517_01365"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX8051611.1"
                     /translation="MTRDEILTPKGLEDLKQKIEFLSTERRREVAERIKEAREFGDIS
                     ENSEYDDAKNEQAMLERQIMELEEKLRGAQVIDESAVSTESVTVGTTVHVKDQKTDKS
                     VKYKIVGSSEANPSDNKLSNQSPVGKALLGKKRGELVSVPVPRGPARKLKITKIEA"
     gene            complement(14159..>14596)
                     /locus_tag="VF517_01370"
     CDS             complement(14159..>14596)
                     /locus_tag="VF517_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX8051612.1"
                     /translation="HKGTPDYALAAELVQCLDAPVIISGGLADDERTLAAFERTGAAA
                     AMLARGSLGNPWRFERLLGLREGEPTQAEVIAELRWVIDRAEEHLGVERAGRYLRKFY
                     PWYAETLGLRKREAAPLVAAPTTADARAVLDRMSSGASAAVAA"
BASE COUNT         1906 a         5273 c         5146 g         2271 t
ORIGIN      
        1 cgccggccac ggtgccgatg ccgctcagca cgccgccgcg gatgtcggcc gcgccgccgc
       61 cgctgctcaa ccgcgagccg gccgacgtca gtcgcgtggt gccgtcgtgc tgcacgtagc
      121 gcgccccgac cgagaggctg ctgtcgcggc cgagcgtcac gccgccgctg ttctcaaagg
      181 tcgcgaccgc gagagcgcga ccgttgtcga gggtcagctc accggcgcgg tcgttcgcgg
      241 tcagctcgcg cagcgcgtcg gcccgcgcga acccgtccac gaccgaggag gccggtccgt
      301 cgagcgtgag ccgcgcggcg ttgcggtcga tccggtctcg cagctcgagc gcggcgcgca
      361 ccctccaggt gccgccgctc agcacgcggc cgcgcaggtt cgtgagcagc ccgctgaacg
      421 cgagcgtccc ctcgtcggcg gccacggtcc cgtcgttcac gaagctcggc gtgatctcgc
      481 cgcgccccgc gccggtcttc gccacgaccc caccggtgag gttgtgcacc accggcgtct
      541 ggtcgcagca gagctcgatc cgcgcggcgt cgacgacctc cacgcggccg gcgttctcga
      601 gccgcgcgcg gtcggcgagc atgaggctcc cgtccgccac gcgcaggccg gcgcccggct
      661 cgatcgcgag cgtgcgccgc tcgaggcggc gttcaccggc gccggacgcc accacgccgg
      721 ggccgccggg cacgatggtg gtactcccgt cgccgaccag gctgccgttc gaccaggcga
      781 cgtcgggtgt ggtgatggtc ccggcaccgg agacgttgcc gttgcgcaac tcgaggcgcg
      841 ggatcgagac ggccgcgtcg aaccagagcg tgccggcgcc gttcacgacg gtgcgcgcgg
      901 tgtaggcgcc ggcggcgtgg acgaccccgc cggcgaagag cacgcggccg tcgccggagc
      961 tggtcgtggt cgacccgggc gcgagcgtgc tgccgccgac cgcgaagcgc acggtcgcgc
     1021 cggcaccggc gtggatcgag ccggtcgact ccccgctgag caccgagacc gttccggcga
     1081 gcacctcgac ggcgcccgcg ttggccagct tgaaggtgac ggtctcgccg ctgccggagg
     1141 ccttgcggat cgtcccgctc gcctcgttca ggatctgcgc ggcggagttg aaccccgagc
     1201 tcgtgaagtc ctggtcgccg cggacctcga gcacgcccgc gttcgcgagc cgcgccgtgt
     1261 cacggatctc gatgtcgccc gcggtccacg tggccgtcgc gccgcgtgcg acctcgaacc
     1321 gacgggcctc gaagagcgag cggatgccgc ccgccagggt ggccccctcg cgcaccacgg
     1381 tcgtgcccgt gccgccctgc cccccggccg accaggtgag gctccccgcg ctgacgcggc
     1441 ccgatccgag taggttcccg cccgactgga ccatcgtcgc caccgagagc tcgccgtcga
     1501 tgcgggccgt cccgctgctc ccgagcgtga ggccgccgat cgacgagctg ccggtggtcg
     1561 tgagcagacc gttgaagacc gtgaccgacc cctcgccacc gagggcggtg acggtgtcgc
     1621 tgccgaagaa gtagcggacc gacgtgcccc cggggatgca ggccgtgtcc gccgggccgg
     1681 gcaggcggtt gccggtccag ttggccgcct cgcgccacac gtcggagccg gcgccgccgt
     1741 cccaggcgat cgtgcaggcg tcctcggccg aggagggcgc acccgtcacc agcgcggcga
     1801 cgagccccgc gagcaccgcg cccacgatcg cgcggcggcg ggagcggaga cggtcgagac
     1861 ggcgggcggg gcggggtgac attcgtggtc ctttcggggt gggctacgaa tgactaaggc
     1921 cggcgcggga gagctcgcca cccgccaaac ggcgggttgc gtcccacaaa cgggcgctct
     1981 ctaccccttt gttgccgcag cctgcggaaa aagccacccg gcggaacgaa gccgcacgct
     2041 gcggaaaaac ggcgtcaggg ggcggcggcg gcgagccgcc gggcgggcgc gtcgagccgc
     2101 cggggcggcg tcgagcggcg ggtgccgcgg tcgcggcggg gggcttacgc ggcggagggt
     2161 tcgaccgtca gcgccggctc ggcgccgccg tcgtcttctt cgtcgtcttc ttcttgccgc
     2221 cgcgatcgac gcgtgtcgtc gggcggccct tcttgcagcc cttgtagcgg cgggtgctga
     2281 tcacgcggtt gccgcgattc gtcagcgcga cgatccgcac cacgtaggtg ccggtgctcg
     2341 gcagcttctt gatcgacacc gtcgtgacct ttgtgccgcg ccggctgtag cggcgcttgc
     2401 cgttgatgta gacgttgacg ttgacgaccc gctggcgcgg cggctggtgg atcttgaact
     2461 tgaacctgcg ccggtcgagg cacttcgact tcggcggcag gccgagcggg ttcgagccgc
     2521 cgccggccga gccggagccg ccgcccgcgc cgccgccacc accgccccca gcggtcgagc
     2581 cgccgggggt gtcccccacc ggctgccgcg gcacgcagct gaagccggtg ggcggcttgt
     2641 cgggcacggc gttcgcgttg cccgcgacct tggtaggcgc gccgttgtag ttgccgtacg
     2701 cgtcgcaggc gtcgccagac tcgatgtaca cgccctcgcc gctcttcagc ttgcggtcgg
     2761 ccacccactc gccgccgacc ttcttgcccg acacgcggtc gaccacgccg ttcttggaca
     2821 cgaacgtgaa caccacccgc tcgaggtcac cgacgtcgag ccacggccgg aacgagcagc
     2881 cgtgcggctt gttcgcgtcc ccggcgtcct gagccggctg gcagtaccac tcgaaccggt
     2941 ccgggtcgtt cggcgcggcc gggtcgcggt aggcgcgccg gtctggcttg atgaaaggga
     3001 tcttcgagcc gtagttcgac tgccacgtgt cggggtagtc gattggcccg agccgcgcct
     3061 cgatgttgaa cgccttgacg gtccgtcgcg ggcccacctt gaacgacggc tggccgccgt
     3121 cggccccgaa gccctcgatg gtcacgcccg tccacgcctt cacgacgaac tccttcgacg
     3181 tgaggtcgta gcgcgcgggc ttgccgtcct gctggcgctg gcccctcaca acgaaccgat
     3241 acgagcccgg cggcgtcgcg accggacggt ccatcaggtc gtagcgcgag acgaacgcct
     3301 cgtagtgcgc ggtccactcg aagcgcgagc cctgcactcg gtaggcgggc cggtcctccg
     3361 acggcgggaa cttgagggtg accggcagct cgccgctctg gtcggcccag tcggtccaat
     3421 taccgccggt gaagcgctgg accttcacct ggggattgtc cgtgtagttc gagccgccga
     3481 cccaggtgaa gaacgacgat ccgaagcgct cgacgtcctc gggctgcttg acgggctccg
     3541 ccttcaggtc gttgggcagc gaggcctcgt acgccgccat cccgcccgtc gcgaactgcc
     3601 cgatcacctg cgcgcggttc tcgttgttct ggaggtccgc ggtggtcttg gcctcgccgc
     3661 ccggcagctc gccgggaagg gggccggtgg gggtgacggt gctgaccacg ttcaggttcc
     3721 ccggcacgac cgggaacggg tccgcctggc cgggcttgag cgagcggccc atcttcacga
     3781 gccgcgtggc catgtagtcg ctcgagtgcg ggccccacgc ggtcagcgcc ttgcggtagt
     3841 ggtcgccgcg ctggtactcg cggtaggtcg cgatgtagcc gttgtagtcg ttgcccatgc
     3901 cgatggcgag cgtgaggccg tacccgaggc cggcgctggc cgcgttgtcg tcgtgggtgt
     3961 agttgccctt gatcagggcc ggatcggtcg gctcgctctc ggcctgggcg acgtaggtga
     4021 ggtcgtccca gcccttcgcg tcgttgttca cctgcgcgcg gatcttctgg tagagcgcgt
     4081 ccgagatcgt ccgctggccg ttgggatgct ggcaggtcca gtcggcgccc gcccgcacgc
     4141 actgcgcggt ccagtcgtag ccgttgtgct ggttgccggc cacacggtcg gtccgtgact
     4201 tgatgttctt cgactggtcc gcccactgct cgcaggagca gaccgtcatc aggatgtcgc
     4261 cgatcctgaa cgcctggagg tggacgctca cgtcctcctc gagcccggtg taggccggcg
     4321 ccgagtagtt ctccgggacg ggcaggccga ggacctggat cgtgtcggtg gtgaccccgg
     4381 ctcccttgat ctcctcgaac gcctgcggcg ggccgttgca gaactcgtcc ggaccgttgc
     4441 cgcggcagtt gtcgtcgaag tactcctggc ggtagtcgct cagcgccgcg caatcgggga
     4501 agccgaccac cggcgcgcgc gggtcgccgg cgagcgccga gtcggtgcgg cacgacgaga
     4561 tccccgggta ggggtgcgac gccggcccgg ggaaccactt gtcggcgaac ttcacgtcgt
     4621 cggccccgaa ctccgacctg aacgggacga agcgctgcgg gttctccggc gtgccggcct
     4681 cgatgtcctt ccacgtcttg acgatgttcg aggcgatccc gcgccccttg aactccgact
     4741 gcgcgtactg cgcgtgcgag aagaaggcgc gctcgtggac gttgtgccag cggttgtcct
     4801 cgggctccgt gttgccgacg gcgccctggg tgtagatcgt caccgccccc gtggcgcgat
     4861 cggtcatccg ctccatcggg cccaggtagt cggcgctgat caggtcgttg ccctcgttgt
     4921 cctccggatg gaccgcgtag ttgacgaggt tcgcgagcgg cttgccgttg tcgagccggt
     4981 cgaagcggac caccatcagg tcgtggtcgg tgtagtcgtt ggggaagccg gccggcgtgc
     5041 cgtcgtcggc ggtcgacggc cccgggacgt tgcgctgcac gaagtcgtac tgcgtgaccg
     5101 aggccccgac cttcaccggg accttcgtcc ggtcggcctc ggctaccgcg gaggccatgg
     5161 tctgcgccat gtactcgaag tagcggaagt cgaacacgtc ctggaagacc cacacgcccc
     5221 agcccgtgga cgagtacagc ggcgacgagt ggttgtgcga cgacgtcatc gtgaggttgt
     5281 cgcggtcgat gtcgatgctc ttgctctcca ggatctgcgc ggtccggcgc cagagcaggt
     5341 cctgcgggat gtagaggtcg ttcttgacga gcgcgaactt cgacccgttc gcgccctgga
     5401 cgacgatcgc gcgggcgctc agccgcgact ggaccccgta ggacggcgcg cgccgcgtgg
     5461 agtgctggtg cggatcggag gagtggacgc tggcgaacgc cccgcccgcg tcgttctcgt
     5521 tcaggtcacc gccggacgcg tactggccgg ccgacgcgcc cacgtgccag gtggcgtcct
     5581 tgaccgccac gcccgccagg atctgcgcgg cgctcgcgcc cgcgggcacg gcgagcagcg
     5641 cgacggcgac cgccagcacc cagcccaaga gtctcgtgat tcgcatcccc cgcagccccg
     5701 gggcggaggg tagcggcgaa agccgccgct gtgcgccggg cacggatcag gccgcgcggg
     5761 cctcggcggc tccgtcgaaa gcccgtgcga gcgccgcgcg gaccgtcttg accgcgcccg
     5821 ccggatgcac cacgtccgcg aggccgaact tcgccgcctc ggcgagccgg cggtcggggt
     5881 gcgcgacgtg ccgcagctcg ccggtgagcc cgatctcgcc gaacgccgcg agcgggcgga
     5941 gcccgtcgcc cgccgcgcgc accccgcgcg cggccgacgc gaccgccagc gccacggcga
     6001 gatcggagcc gggctcctcc acccggacgc ccccggccac cgacacgaac acgtcgctcc
     6061 cgccgagcgc gaccccgccg tggcgagcga gcaccgcgag caccagcgac agccggttgc
     6121 ggtcgatccc gttcgcgatc cgccgcggcg gctccgattg agccggcgcg accagcgcct
     6181 gcacctcgac cagcagcggc cgcgacccct ccatcgcgca cagcaccacc gaccccggag
     6241 cgcgcgtggc ctcctcgacg aagcgcgccg aggcgtcggc cacctccacg aggccgctct
     6301 cgcgcatctc gaagacgccc gtctcgttcg tggagccgaa gcggttcttc agcgcgcgca
     6361 gcgtccggta ggtgcgctcg cgctcgccct cgaactggag cacgcagtcg accaggtgct
     6421 cgagcacccg cgggcccgcg agcgcgccct ccttggtcac gtgccccacg agcaccgtgg
     6481 ccacgccacg ctccttggcc acgcgagcga gccggcccgc cacctcgcgc acctggccta
     6541 cggagccggc ggtgccgctg aggtcggcgc actggagcgt ctgcaccgag tccacgacgc
     6601 acacgtccgg gcgctcggcc tccagggtgg cgaccaccgg ctcgaggctc gtctcggcga
     6661 cgatcggcac gcgcagcgcg tcgggtccga gccgctccgc gcgcagcttc acctgtgcgg
     6721 ccgactcctc gcccgacacg tacaggaccg accgcccggc ggcggccagg ttgccgagcg
     6781 ctgaggccgt gatcgtggac ttgccgatgc cgggcgaacc gcccagcagc accaccgatc
     6841 cggggacgag cccaccgccc agcacgcggt ccagctcccc gatgccggtg ccgagccgcg
     6901 gcacgcgcgg cgcctcgacg tcggccagcg ccacggggcg cagcgccgga gccgcgcgct
     6961 tcgcgcggcc ggcgggagcg gcggccggca cctcctcgac cagcgtgttc cactcgccgc
     7021 agccggggca ctggccgtgc cacttcacgc tctcgtgagc gcaggcgctg cagacgtgga
     7081 ccgagcgagg catcccgcca cggtaaggcg cgcggaggac gctttcgccc cccggggagg
     7141 ttggatgctc gaccggcgga ggctcactac gccggtcgag ccgttctagg ttcgcggtcg
     7201 gcgcatcgga acctcccgcg cggactaccc tcgcccgcat gacccctcgc cgcccgtttc
     7261 tcgccctcct cgccgtcgcc gcagccgcgc tcggggccag ccccgcggcg gccgccgtcg
     7321 cgaccggccc gtccggagac gccttctaca ccccgccgaa gaagctcccc ggcaagacgc
     7381 acggcgaccc gatctggatc cgcaagctca ccaaccagca gacgctggcc gaggcgaagg
     7441 tgaacgagtc ggttctgtac cgctcgacct cggtgcgcgg caagacggtc gccgtgtccg
     7501 ggacgatcca cctcccgaag ggcaaggcgc ccaggggcgg ctggccggtc gtgtcgtggg
     7561 cgcacgccac gagcggactc ggcgaccagt gcgccccgtc gcgcaacccg ggcgagggcg
     7621 cgatccacgc ctaccacgtc tacatcgagg cgctgctcga ccgctggctg aaggccggct
     7681 tcgccgtggt gcgcaccgac tacgaggggc tcgggaccaa gggcgtccac ccgtacctcg
     7741 tcggcgtctc cgagggacgc ggcgtgctcg acatcgtccg cgcggcgcgc aaggtccacc
     7801 ctgggctgct gagcacgcgg ttcgcgatcg gcggccactc gcagggcggc cacgcggccg
     7861 tgttcgccac cgcgctcgcg cggaagtggg tgccggagct gtcgctgcgc ggcaccacgg
     7921 ccttcgcgcc gttctcgcag ggcgacgaga tcttcgcctc gctcgcgcag ctcgaccagc
     7981 cctcgcgcgt gagcggctac gcggcgctct tcgtccgtgg gatcgaggcc ggctacccgc
     8041 agctcggcgt cacgtcgctg ctctcgcagc gcggcgccga cctctacccg ctgacgctgt
     8101 cggtctgcat cgacgagatc acctccgaga gctcggagtt cgcgaagacg gctccgcgcg
     8161 acctcttccg cgagggtgcc gacctcgagc cgatcaggac gacgctcgcc gccaacgacc
     8221 cgaccgacct gaagctgacc acgccgctgc tggtcgaaca gggcagctcg gacacgacgg
     8281 tgaacccgaa cttcacccag gcgatggtcg cggggctccg caagagcggc gcgaaggtca
     8341 ccctcaagct gcacgagaac atcgaccaca ccacggtcgc gttcggcgag ccgcaggacg
     8401 aggccttcag ctgggtctcg aagcgcctgc gctagctact cgtcggcagc gcgcacggcg
     8461 cgcgtagcgg catcgaaccc gatcttcgag tgcgtgccgt cgcagaacgg cttcgtggtc
     8521 gacccaccgc agcggcagag agcgaccggc cgcccgtcgt ccgggatctc gaacctgttg
     8581 ccgtcggcgt cgacgagcgt gatcgggccg gtgacgcggt agggcccgtt ctcgcgaacc
     8641 ttgatctcga cgggatcggg catgcgacga gtcaccctac aggggggcga aaacccccgc
     8701 gcagggcctt agacccctgt gtatggagga gtacgatgac cgcgatgccg gccgctggcc
     8761 tgatgaccgc agacgaattc ctcgcgctgc cccaggcgcc gcgccacgag cgctggagcc
     8821 tggtcgaggg tgagctcgtg atggagcagc ccctgccgag ccatcaggtc gcgtgcgtgg
     8881 agatcctgta tgccctcgag agttgggtcc gttcgcgacc cgggcgtggt cgggcgctca
     8941 taccgatcga cgtcgagctg gacgagcgga acatctacgc gccggacgtg ctctggtacg
     9001 cggagggccg cgaccctgcc cgggcgaacg tgcgcccgtc gccgatcccc gatctcgccg
     9061 tcgaggttcg ctcgccgtcg acctggcgct acgacatcgg cgcgaagaag tcggtctacg
     9121 agcgccgcgg acttccggag ctgtggctcg tcgacaccgc cgccagcgtc gtgctcgtgt
     9181 ttcgccgctc ggcaaccggg gccccgcgct tcgatgtcgc cctcgagctc accgcggccg
     9241 actcgctcga gtcgccgctg ctgcccggct cgcgctgccg cttgcgcggc tcctcgcgga
     9301 ctaggactcg gagggcgggc ccggctcgtc gctcggcggc gcgtccggcg cctccggcag
     9361 cacgtcgggc acgtccggca gcgagccggt gtccggcagt gagccctggg actcgtcgcc
     9421 gccggcctcc gcctcgccgt cgccgccgac cgtgaccggc tcgcgcttcg gagccgtgat
     9481 cgagatcttg acctcgggct cctcgccgtc gccgccctcg gcgcggtcga cctcgaccgt
     9541 cgcgcccggc tgcatgtcgt cggccttcag cacctcgtcg gcaagcgggt cctcgatgta
     9601 gcgctggatc gcccggcgca gcggacgggc tcccatcgcg ggatcccagc ccttctcgac
     9661 cagcaggtcg cgcgcgtcgt cggagaggtt gagcgagagc tcgcgctccg ccatcgactc
     9721 gcggatccgc cgcaggagga gctcgacgat ctcggtgatc tcctccttgg tgagcttgtg
     9781 gaagacgatc acctcgtcga tgcggttgag gaactcgggc cggaagagct tcttcagctc
     9841 gcccatgatc cggttcttca tgtcgtcgta ggtgatgccg gtgtcgtcgc cgatcgtgaa
     9901 gcccacgccc gtgttcttcg cgatctccga ggcccccacg ttcgaggtca tgatcacgat
     9961 cgcgtgacgg aagtcgaccg tgcggccctg cgcgtcggtg agccggccgt cctccaggat
    10021 ctggagcagc acgttgaaca cgtccgggtg ggccttctcg atctcgtcga ggagcagcac
    10081 cgagtacggc ttgcggcgca cggcctcggt gagctggccg ccctcgtcgt agccgatgta
    10141 gccggggggc gagccgacga gccgcgacac ggagtgcttc tccatgtact ccgacatgtc
    10201 gatgcgcacc atcgcgtcct cgtcgccgaa caggaactcg gcgagcgtgc gcgccagctc
    10261 ggtcttgccg acgccggacg gcccgaggaa gatgaacgag ccggtcgggc gcttcgggtc
    10321 cttgagcccg gcgcgcgagc ggcggatcgc cttcgacacg gcctcgatcg cggcctgctg
    10381 cccgatgacg cgcttgtgca gctcgtcttc catgcgcatc agcttctgcg tctcggcctc
    10441 ggtgagcttg aagaccggga tgccggtcca catcgagacg atgtcggcga tctcctcctc
    10501 gccgatcgcg gggcgctcgc cggactcgcc ggcgcgccac tgctcctcga gctcgcgctt
    10561 cttgttggtg agctggcgct cctggtcgcg caggttcgcg gccttctcga actcctgcgc
    10621 ctcgatcgcc gcctccttgt cgcgccgcgt ggtctcgatc tcctcctcga ggtcgcggta
    10681 cacgggcggg gcggtcatcg acttgatccg catgcgcgac gcggcctcgt cgatcaggtc
    10741 gatcgccttg tccggcagga agcggtcgga gatgtagcgg tcggccagct cggccgcggc
    10801 gtgcagcgcc tcgtcggtga tgtcgacgcg gtggtgctgc tcgtagcggt cgcgcagccc
    10861 cttgaggatc tgctcggtct cctcgatcga cggctggtcg acgcggatct gctggaagcg
    10921 gcgctcgagc gcggagtcgc gctccaggta cttgcggtac tcgtcgagcg tggtggcgcc
    10981 gatcgtctgg agctcgccgc gcgcgagggc cggcttgagg atcgaggcag cgtcgatcgc
    11041 gccctcggcc gcgccggcgc cgacgaggtt gtgcagctcg tcgatgaaca ggatgatgtc
    11101 gccgcgctgg gtgatctcct tcatcacctt cttgaggcgc tcctcgaact cgccgcggta
    11161 cttcgagccc gcgacgagcg ccgccaggtc gagcgtgtag atctgcttgt tcttgagcag
    11221 ctccggaacc tcaccgtgcg tgatccgctg cgcgaggccc tcgacgacgg cggtcttgcc
    11281 gacgccgggc tcgccgatca gcactgggtt gttcttcgtg cggcgcgaga ggatctgcat
    11341 gatccgctcg atctcggtct cccgacccac gacggggtcg agcttcgcct cggcggcgag
    11401 gcgggtcagg ttgcggccga actggtcgag cagcttcgag gacttcttgc cctcgccctg
    11461 ctgaccctgc gcgccctggc cctgctggcg gcgaccgccc gggccggaga gcatccggat
    11521 gacctcgttg cggatcttct cggagtcggc gtcgaagtcg agcaggatgc gcgcggccac
    11581 gccctcgttc tcgcgcacga ggccgagcag gatgtgctcg gtgcctatgt agttgtgacc
    11641 gagcgagagc gcctcgcgca gagcgagctc cagcaccttc ttcgcgcgcg gggtgaacgg
    11701 gatctggccg gaggtgacct cctcgcccga tccgacgatg cggacgacct gcgcgcgcac
    11761 gcgctccacg gtgatgtcca gcgactcgag cacacgcgcg gcgagaccct cctcctcgcg
    11821 gaggagaccg agcaggatgt gctcggtgcc gatgtagttg tgcttgagga tccgggcctc
    11881 ctcttgcgcg aggacgacca cttggcgggc ccgttcggtg aagcgctcga acacggtgcg
    11941 gtccttaact ctcggggtac cccctgggag ggaaacccgg gggttcttcc gtcggtatgg
    12001 gcaccttcgc cacccgttcc aagatccctg gcctcccgac ccgtgggagg agtcgagtct
    12061 agcaacggga accggccgcg aaagttgacg aattagccct taacgcgggg tacgtaccgg
    12121 ccccgcgagc gggtcaattc cgcatcgcgg ggaacaggat gacctcgcgc agggtggtcc
    12181 tcccggcgag cagcatcacg agcctgtcga tcccgagccc gacaccgctc gtcggcggca
    12241 tcccgtgctc gagcgcgcgc acgtaatcct cgtcgtaggg ctgcgtctcc tcgtcgccct
    12301 cggcgccgag gcgctgctgc tcctcgaagc gcgcgcgctg gtcgtcgggg tcgttcagct
    12361 cgctgaacgc gttcgcgatc tcgatgcccc cggcgaagca ctcccagcgc tcgacgagcc
    12421 cgtcctcgga gcggtggcgc ttcgccagcg gcgacagcgc gaccggatag tccatgacga
    12481 acgtcgggtt catgagcttc ggctcgacgt gcttcgacag cagctcgtcc acgagctgcg
    12541 gccacgactc ggtggggtcg agctcgatgc cgcgctcctt cgcggcggcg acgagcgtct
    12601 ccgtgtcctc ggccgcggac acgtccacgc ccgtggcgtc gagcaccgcg tcgcgcagct
    12661 tgaggcgcgc ccacggacgc tgccagtcga tgccctcgcc gggcgcgcca ctcgcgccga
    12721 ccacgtcggt gacgagctcc tcgagccgct cggccccggt ctcgtagtcg ccgtaggcct
    12781 cgtaccactc gagcatcgtg aactcggggt tgtgcttgtg cgagatgccc tcgttgcgga
    12841 agtccttgcc gagctcgtac acgcgctcga ggccgccgac gatcagccgc ttcaggtaca
    12901 gctcggtggc gatgcgcagg tagaggtcgc ggtcgagcgc gttgtggtgg gtcacgaacg
    12961 ggcgcgccat cgcgccgccg tagagcggct ggagcacggg cgtctcgacc tcgaggaagc
    13021 cgcggtcgtc gaggtggcgg cggatcctcg acacgacctg cgagcgcagc aggaagagcc
    13081 ggcgcgagtc ctcgttcgcg atcaggtcga gctcgcgctg gcggtagcgg gtctcgacgt
    13141 cctggaggcc gtggtacttg tcgggcggcg ggcgcagcga cttcgcgagc agcgtccagc
    13201 cgtcggcgcg cagcgacagc tcgccgcggc gcgacttgaa cgccgtgccg tcgatgccga
    13261 ccaggtcgcc gaggtcgagc gacagcaggc gttcgaactc catcgggcca aggacgtcca
    13321 cgcgcgcgtg gacctgtatc cgccccgacc ggtcgacctc gtcgaggaac gccgccttgc
    13381 cctgcccgcg ccgcgcgctc agccggcccg cgacccggta ggtcgcctcg gtctcctgac
    13441 ccgcctcgag ctcgccgtgc gcgccgtgca ccgactcgat cgcgacggcg ccggggtacg
    13501 cgtgcgggaa cgggtcgatg ccctcggcgc gcagccgctc gagcttcgcg cggcggtcgt
    13561 cgagtgcctg cgcctcggtc ttgggctgct cggtcacggc tcggcactgt aggcgccgcg
    13621 ccgggcgcct cgcgggctac gcctcgatct tggtgatctt cagcttgcgc gcggggccgc
    13681 gcgggaccgg cacggacacc agctcgccgc gcttcttgcc gagcagcgcc ttgccgaccg
    13741 gcgactggtt cgagagcttg ttgtcggagg ggttggcctc cgaggagccg acgatcttgt
    13801 acttgaccga cttgtcggtc ttctggtcct tcacgtgcac cgtcgtcccg accgtcacgg
    13861 actcggtcga gaccgcgctc tcgtctatca cctgggcgcc gcgcagcttc tcctcgagct
    13921 ccatgatctg gcgctcgagc atcgcctgct cgttcttcgc gtcgtcgtac tcggagttct
    13981 ccgagatgtc gccgaactcg cgcgcctcct tgatccgctc ggccacctcg cggcggcgct
    14041 cggtggagag gaactcgatc ttctgcttga ggtcctcgag ccccttgggg gtgaggattt
    14101 cgtctcttgt catcaggaaa agcgctccta cgaagggcga agggcgcgac aggatagctc
    14161 aggcagccac ggctgcggac gcgccactgg acatacggtc gaggaccgct cgggcgtccg
    14221 ccgtggtggg cgccgccacc agcggcgcgg cctcgcgctt gcgcagcccg agcgtctccg
    14281 cgtaccacgg gtagaacttc cgcaggtaac ggcccgcgcg ctcgacgccc aggtgctcct
    14341 cggcccggtc gatcacccag cgcagctcgg cgatcacctc cgcctgcgtc ggctcgccct
    14401 cgcgcaaccc gagcaggcgc tcgaacctcc acgggttgcc gagcgagccg cgggccagca
    14461 tcgcggcggc cgcgccggtc cgctcgaagg cggcgagcgt gcgctcgtcg tcggccaggc
    14521 caccggagat gatcaccggc gcgtcgaggc actggacgag ctcggcggcg agcgcgtagt
    14581 cgggcgtgcc cttgtg
//
LOCUS       DASVPL010000027         7631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_198124, whole genome shotgun sequence.
ACCESSION   DASVPL010000027 DASVPL010000000
VERSION     DASVPL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198124"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1364)
                     /locus_tag="VF517_01375"
     CDS             complement(93..1364)
                     /locus_tag="VF517_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8051613.1"
                     /translation="MYRLAILVVTAACAALLLAPAAQAGRVVPVQLGQKSGEVIFANG
                     QPSAPDPAILVHLEGLIDRAAPDSTIVASIYAITRNGRIEDALRRAYTDPARRIRVQI
                     VSNESGLKPGEPFDDWPASDRFKFKNCSGGCISLTNPENGNASIAHSKFFVFQATRRT
                     ATSPIVPVSWVASANLDANTGTDAWNDAITWYDDPDMNWGLYTVFLDMWNGPYYTKGF
                     PESHPWFDYFTWPNDNPTTAGNGAFFAPDSASTGIVSPELDSDLWVTQLSMIKGPGAG
                     GAPVCRVAVLHNKIFKERIAVVRQLRRLVNEDCVVRVLVNRTVGSADDPAHPEIDEPS
                     RTELCAPTNGDLQVGATTKLHHKAIISYGTFYGVSRPVVWAGSHNLSEVALKANDEVI
                     VRVDGSQSLWDAYAQQFLDAWNARAVTICTP"
     gene            complement(1467..2810)
                     /locus_tag="VF517_01380"
     CDS             complement(1467..2810)
                     /locus_tag="VF517_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8051614.1"
                     /translation="MAEQLAEVVGAPHVLVDDDVRAAYETDWTRRFHGRAAAVVRPGS
                     AAEVAGVLGVCAAHGVAVVPQGGNTGLVGGSVPRGGEVVLLLTRLTGLGEVDRASLQV
                     DAGAGVTLAALQDHARAAGLDAGVDLAARDTATVGGLVACDAGGLRALRHGTVRARVA
                     GLEAVLADGSVVRRMSGLMKDNAGYDLPALLVGSEGTLGVVTRVRWQLVARLDARALA
                     FVPLASLGAAAELLAELRPKLPSLIACEFITDDGLTLVLDHLDVRAPVDERAPAYVLL
                     ECGAAKDPLEELASALDGAGLGHALLVDDGASRDRLWRLREAHTEAIAAAGIPHKLDV
                     GVPLARLGEFAERVAGLLADVAPGARPILFGHLGDGNVHVNLLGLDPGDEAADVAVLR
                     LATECGGTISAEHGVGVAKARHLGLARSDEEIAAMRAIKRALDPGGLLNPGAVLP"
     gene            2827..4095
                     /locus_tag="VF517_01385"
     CDS             2827..4095
                     /locus_tag="VF517_01385"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEX8051615.1"
                     /translation="MGHETVFTLEATPVKFGPGAAAEAGWELKRLGVRRVLLVTDPGV
                     AAAGHADRVRESIAAEGIEVVVYDESRVEPTLESLEHACEAARDARVDGFVSVGGGSA
                     MDTAKVANLVDSHPAPVMDYVNPPVGEGRKPPTPLKPHLAMPTTSGTGSEATTVAVLD
                     IPHLKVKSGISHRYMRPSQAIVDPELTRTLGPEVTSSAGLDVVCHAAESYISRPFDSR
                     PRPASPDDRPPYQGSNPVADVWSAKALEFGGRYLRRAVADPGDVEARGRMMLAATMAG
                     VGFGSAGVHIPHACAYPIAGLKHEYEPPGYPTDHPFVPHGHSVIVTAPAAFRFTYDAD
                     PERHHRAAELLSGERVTDPGPDTLPDVLLELMRDVGAPSGVAELGYGEDDVPALVEGA
                     LKQQRLLVVAPKEAGADDLGHIISASMRNW"
     gene            4092..4520
                     /locus_tag="VF517_01390"
     CDS             4092..4520
                     /locus_tag="VF517_01390"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8051616.1"
                     /translation="MSSDYPHTLTIQTRWKDNDVYGHVNNVEYYSYFDTAINAWLIGE
                     GGLDIEGGEVIGVCAESFCKFSSGVAFPDAVDVHLRVGKLGRSSVRYELAVARAGEPD
                     PAATGHFVHVFVDRESRRPVPIPDRLRAALERLVPAAGGG"
     gene            complement(4528..6060)
                     /locus_tag="VF517_01395"
     CDS             complement(4528..6060)
                     /locus_tag="VF517_01395"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX8051617.1"
                     /translation="MKSIVPLILCAVALVAAAPAHAVVEVAGTGEPAYTNTTTNTQWV
                     SWQAPAGATQYRLRVTYYENNVQVQQETTGNVSASGTTWVNWAGVRSPLVEGKTYAIC
                     VQGEYTIAGDGTFSPDGSSCANGSATGKRTSTTIDRTAPAVTTTLADGAAAVKTATFG
                     MQIGFQDNLAGPYPANFVCLKAVESGTADTDCTYSLSDACSIPATGGKDTTFGCQVNV
                     SGLPDGPVSACVIGTDAAVPDNPTSSTQTSTASQANRATAACDRVTLDRVAPSVAISS
                     SKATATVGETISFSSQASDATSGLAGAGGWQFGDGATGAGAAADHSYAQAGTYTVRYT
                     VTDGAGNSATAEKVVTVTAPATSDPGTQNPGTQNPGTQNPGTQNPGTQNPGTQDPGTQ
                     NPGTQDPGPTPAPGSVQVGSVWVLAPKRFRIGSSRRLSVSVGASQAGQLTLTLVRGKK
                     TIARLGVGLSPGATKQAIKLPKKLAAGTYSLKIGFKPSGVTWSASGSAKIAFQAAKKK
                     KR"
     gene            6183..6833
                     /locus_tag="VF517_01400"
     CDS             6183..6833
                     /locus_tag="VF517_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051618.1"
                     /translation="MPRPPALALALMPLALAGCGDGPRSGEPAATDPCRGVGPVTVAS
                     DGTGDVSANRAVPKPPPPPAGIDLVQVQVARSATKLCAVFESEAPPPKTGGFRVGLRE
                     RGKGDSGTLVGVNAFRREGEWTVELGYPGADERADRGIVDADVEVRGNRVSLVMDTRT
                     LPEATPPASGSEWEASTLGVGDSPNLQYVDCAPESTQHITYPGGELVSFGNGRGSC"
     gene            complement(6843..>7631)
                     /locus_tag="VF517_01405"
     CDS             complement(6843..>7631)
                     /locus_tag="VF517_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051619.1"
                     /translation="EARAFGEWLDENFDAIREVAESTTSTGKLQDIEQYSASRILYTR
                     FNYTTGDAAGQNLTGKATQAACKWITENYPGIEHYFLESNFATDKKSSQVNMLNTRGK
                     RVIAEATIPNELFQRIMRSDSALMYRARQVSNLGGFMSGVNNNGAHSANGITAMFIAT
                     GQDAANVAESSAAFVYAELRPNGDYYYSVTIPSLIVASYGGGTGLATQRECLEMLGCY
                     GKDRVRKLTEIVAATVLCGELSLGSAIVAEEWVEAHDLFGRNRP"
BASE COUNT         1025 a         2718 c         2774 g         1114 t
ORIGIN      
        1 gcgccgccgg gggcgatcat cggcggccgc cgggggcgat catcggcggc ggccgggggc
       61 gaccatcggc cgctgggggc gatcccccgg ccctagggcg tgcagatcgt gacggcgcga
      121 gcgttccatg cgtccaggaa ctgctgggcg taggcatccc acagcgactg cgacccgtcc
      181 acgcgcacga tcacctcgtc gttcgccttc agggccacct ccgagaggtt gtgcgacccg
      241 gcccagacca ccgggcgcga gaccccgtag aaggtgccgt agctgatgat cgccttgtgg
      301 tgcagcttgg tggtcgcgcc gacctggagg tcgccgttcg tgggggcgca gagctccgtg
      361 cgtgacggct cgtcgatctc cgggtgtgcg gggtcgtccg cgctcccgac cgtgcggttc
      421 acgagcaccc gcaccacgca gtcctcgttg acgagccgcc gtagctgacg gaccaccgct
      481 atccgctcct tgaagatctt gttgtggagc acggcgacgc ggcagacggg cgcgccgccc
      541 gcgcccggcc ccttgatcat cgagagctgg gtcacccaga gatcgctgtc cagctccggc
      601 gaaacgatcc ccgtgctcgc cgagtcgggc gcgaagaacg cgccgttgcc agcggtcgtg
      661 gggttgtcgt tcggccaggt gaagtagtcg aaccacggat gcgactcggg gaagcccttc
      721 gtgtagtacg ggccgttcca catgtcgagg aacaccgtgt acaggcccca gttcatgtcg
      781 gggtcgtcgt accaggtgat cgcgtcgttc cacgcgtcgg tgccggtgtt ggcgtcgagg
      841 ttcgcggacg cgacccacga gaccgggacg atcggagacg tcgccgttcg cctggtcgcc
      901 tggaagacga agaacttgct gtgggcgatc gaggcgttgc cgttctcggg gttggtgagc
      961 gagatgcagc ctccggagca gttcttgaac ttgaagcggt ccgacgcggg ccagtcgtcg
     1021 aacggctcgc cgggcttgag gcccgactcg ttggacacga tctgaaccct gatccgccgc
     1081 gccgggtcgg tgtaggcccg tcgcagggcg tcctcgatcc gcccgttcct cgtgatcgcg
     1141 tagatcgagg cgacgatcgt cgaatcgggt gcggcgcgat cgatgagccc ctcgaggtgg
     1201 accaggatcg ccgggtcggg ggcgctcggc tggccgttgg cgaagatcac ctcacccgac
     1261 ttctggccga gctgcacggg cacgacgcgc ccggcctgcg cggcgggtgc cagcagcagg
     1321 gcggcgcacg ccgccgtgac gacgagaatg gccagtcggt acatcacgaa tccccctctt
     1381 cagcgttaaa gcgaccctaa gcgcacggag ggtctgaatg caagggccaa ccgtgcgggg
     1441 ccgccgtcag ggagtacggg ccgccgtcag ggcaggacgg cgcccgggtt cagcagcccg
     1501 cccgggtcga gcgcgcgctt gatcgcgcgc atcgcggcga tctcctcgtc cgagcgcgcc
     1561 aggccgaggt ggcgcgcctt cgcgacgccg acgccgtgct cggcgctgat cgtgccgccg
     1621 cactcggtcg cgagtcgcag gacggcgacg tcggccgcct cgtcgccagg gtcgaggccc
     1681 agcaggttca cgtgcacgtt gccgtccccg aggtggccga acaggatcgg gcgcgcgccg
     1741 ggcgcgacat ccgcgagcag cccggcgacc cgctccgcga actcgcccag ccgcgcgagc
     1801 ggcacgccca cgtcgagctt gtgcgggatc ccggcagccg cgatggcctc ggtgtgggcc
     1861 tcgcgcaggc gccagagccg gtcgcggctc gcgccgtcgt ccaccagcag cgcgtggccg
     1921 agcccggcgc cgtcgagcgc gctggccagc tcctcgagcg ggtccttggc cgcgccgcac
     1981 tcgagcagca cgtaggcggg cgcgcgctcg tccaccggcg cgcgcacgtc gaggtggtcg
     2041 agcacgagcg tgagcccgtc gtcggtgatg aactcgcacg cgatcagcga cggcagcttc
     2101 ggccgcagct cggcgagcag ctccgcggcc gcgccgagcg aggccagcgg cacgaacgcc
     2161 agcgcgcgtg cgtcgagtcg ggcgacgagc tgccagcgca cccgcgtgac cacgccgagc
     2221 gtgccctcgg agccgaccag cagcgcgggc aggtcatagc ccgcgttgtc cttcatcagg
     2281 ccggacatcc gtcgcaccac cgagccgtcg gcgagcacgg cctcgagccc ggccacgcgt
     2341 gcgcgcaccg tgccgtggcg cagcgcgcgc aggccgcccg cgtcgcaggc cacgaggccg
     2401 ccgacggtcg cggtgtcgcg tgcggcgagg tccacgcccg cgtcgaggcc cgcggcgcgc
     2461 gcgtggtcct gcagcgccgc gagcgtcacg cccgcgccgg cgtcgacctg aagcgacgcg
     2521 cgatcgacct cgcccaggcc ggtcaggcgc gtgagcagca gcaccacctc gccgccgcgc
     2581 ggcaccgagc cgccgaccag ccccgtgttg ccgccctgcg gcacgaccgc cacgccgtgt
     2641 gccgcgcaga ccccgagcac gcccgcgacc tccgcggcgg agccggggcg cacaaccgcg
     2701 gcggcgcgcc cgtggaagcg ccgcgtccag tcggtctcgt acgcggcgcg cacgtcgtcg
     2761 tcgactagca cgtgcggggc gccgaccacc tcggcgagct gttctgccaa tctcacgccc
     2821 tcaactatgg gacatgaaac tgtgttcacg ctcgaggcga cccccgtgaa gttcggcccg
     2881 ggggcggccg ccgaggcggg ctgggagctg aagcggctcg gcgtccggcg cgtcctgctc
     2941 gtcaccgacc ccggcgtggc ggccgcgggg cacgccgacc gcgtgcgcga atcgatcgcc
     3001 gccgagggca tcgaggtggt ggtctacgac gagagccggg tcgagccgac gctcgagtcg
     3061 ctggagcacg cgtgcgaggc ggcgcgcgac gcgcgggtcg acgggttcgt gtcggtcggc
     3121 ggcggctccg cgatggacac ggcgaaggtg gcgaacctcg tggactcgca cccggcgccg
     3181 gtgatggact acgtgaaccc gccggtgggc gagggccgca agccgccgac cccgctcaag
     3241 ccgcacctcg cgatgccaac cacctccggc accggcagcg aggcgaccac cgtcgcggtg
     3301 ctggacatcc cgcacctcaa ggtcaagagc ggcatctcgc accgctacat gcgaccgtcg
     3361 caggcgatcg tcgacccgga gctgacccgc acgctcgggc cggaggtgac ctcgtcggcg
     3421 gggctcgacg tcgtgtgcca cgcggccgag tcgtacatct cgcggccgtt cgactcgcgc
     3481 ccgcggccgg cctcgccgga cgaccgcccg ccctaccagg gctccaaccc ggtggccgac
     3541 gtctggtcgg cgaaggcgct cgagttcggc ggccgctacc tgcgccgcgc ggtggcggac
     3601 cccggcgacg tcgaggcgcg cgggcggatg atgctggcgg ccacgatggc gggcgtcggc
     3661 ttcggctccg cgggcgtgca catcccgcac gcctgcgcct atccgatcgc ggggctcaag
     3721 cacgagtacg agccacccgg ctacccgacc gaccacccgt tcgtgccgca cgggcactcg
     3781 gtgatcgtca cggcgccggc ggccttccgc ttcacctacg acgcggatcc cgagcgccac
     3841 caccgcgcgg ccgagctgct ctcgggcgag cgggtcaccg atccgggccc ggacacgctg
     3901 ccggacgtgc tgctcgagct gatgcgcgat gtcggtgctc cgagcggcgt ggcggagctc
     3961 ggctacggcg aggacgacgt gcccgccctc gtggagggcg cgctcaagca gcagcggctg
     4021 ctcgtggtgg cgccgaagga ggccggtgcc gacgacctcg gccacatcat ctcggcctcg
     4081 atgcgcaact ggtgagctcc gactacccgc acaccctcac gatccagacc cgctggaagg
     4141 acaacgacgt gtacgggcac gtcaacaacg tcgagtacta ctcgtacttc gacaccgcga
     4201 tcaacgcgtg gctgatcggc gagggcgggc tcgacatcga gggcggcgag gtgatcggcg
     4261 tgtgcgccga gtcgttctgc aagttcagct cgggcgttgc gttcccggac gccgtggacg
     4321 tgcacctgcg cgtcggcaag ctcgggcgct cgagcgtgcg ctacgagctg gcggtggcga
     4381 gggcgggcga gccggacccg gccgcgaccg ggcacttcgt tcacgtgttc gtggaccgcg
     4441 agtcgcgccg cccggtgccg atccccgatc ggctccgagc ggcgctcgag cggctcgtcc
     4501 cggccgccgg cggtggctag ccgaccgcta gcgcttcttc ttcttggcgg cctggaaggc
     4561 gatcttcgcg ctgccgctgg ccgaccacgt cacgccactt ggcttgaagc cgatcttcag
     4621 cgagtaggtc ccggcggcga gcttcttggg cagcttgatc gcctgcttcg tggcgccggg
     4681 agagaggccg acgccgagcc gcgcgatcgt cttcttcccg cgcaccaggg tgagggtgag
     4741 ctggcccgcc tggctcgcac ccaccgagac gctcagccgc cgcgagctgc cgatccggaa
     4801 gcgcttcggc gcgagcaccc agacggagcc gacctgcacg gagccgggcg cgggggtggg
     4861 ccccgggtcc tgagtgccgg ggttctgcgt ccccggatcc tgagtcccgg gattctgagt
     4921 cccgggattc tgagtcccgg ggttctgcgt gcccgggttc tgcgtgcccg ggttctgcgt
     4981 gcccgggtcg ctggtcgcgg gcgccgtcac cgtcaccacc ttctcggcgg tggccgagtt
     5041 tccggcgccg tcggtcaccg tgtagcgcac ggtgtaggtg cccgcctggg cgtagctgtg
     5101 gtcggcggcc gcgccggcgc cggtcgcgcc gtcgccgaac tgccagccgc cggcgcccgc
     5161 cagcccggag gtcgcgtcgc tggcctgcga ggagaacgag atcgtctcgc cgacggtcgc
     5221 ggtggccttc gacgagctga tcgccacgct gggcgccacg cggtcgagcg tgacccggtc
     5281 gcaggcggcc gtcgcgcggt tcgcctggct cgcggtcgac gtctgcgtgg agctggtcgg
     5341 gttgtcgggc accgccgcgt cggtgccgat cacgcaggcc gagaccggac cgtcgggaag
     5401 acccgacacg ttcacctggc agccgaaggt ggtgtccttc ccgcccgtgg cgggaatgct
     5461 gcacgcatcc gacaacgagt acgtgcagtc ggtgtccgcg gtgcccgact cgacggcctt
     5521 gaggcaaacg aagttcgcgg ggtagggccc ggcgaggttg tcctggaacc cgatctgcat
     5581 gccgaaggtc gccgtcttga cggccgcggc gccgtccgcg agcgtcgtcg tgacggccgg
     5641 cgcggtccgg tcgatggtgg tggatgtgcg cttgcccgtg gcggacccgt tcgcgcacga
     5701 gcttccgtcc ggcgagaacg tcccgtcgcc ggcgatcgtg tactcgccct gcacgcagat
     5761 cgcgtaggtc ttcccctcga cgagcgggct gcgtacgccc gcccagttca cccacgtggt
     5821 gccgctcgcc gacacgttgc cggtcgtctc ctgctgcacc tggacgttgt tctcgtagta
     5881 ggtcacgcgc agccggtact gggtcgcgcc cgccggagcc tgccatgaga cccactgggt
     5941 gttggtggtg gtgttggtgt acgccggctc accggtgccg gcgacctcga cgaccgcgtg
     6001 ggccggggct gccgccacga gcgcgacggc gcagaggatg agggggacga tcgacttcat
     6061 cacgtagggg tgcgtccacc gtaagcaccc aacggacgac gcgccagtac cccgcgatgt
     6121 gcgattgcgc caagatgggg tcatggcggt gaaggtgccc ggccctaccc tgagccgcga
     6181 caatgcctag gcctcctgcg ctcgcgctcg cgctgatgcc actggcgctc gccggctgcg
     6241 gtgacggccc gcggtccggc gagcccgccg cgaccgaccc gtgccggggc gtggggccgg
     6301 tgaccgtggc aagcgacggc accggggacg tcagcgccaa ccgggcggtt cccaagcccc
     6361 cgccgccgcc cgccggcatc gacctcgtcc aggtgcaggt ggcgcggagc gccacgaagc
     6421 tctgcgccgt gttcgagtcc gaggcgccgc cccccaagac cggcgggttt cgcgtcggcc
     6481 tccgggagag ggggaagggc gacagcggca cgctcgtcgg cgtcaatgcg ttccggcgcg
     6541 agggcgagtg gaccgtcgag ctcggctatc ccggagccga cgagcgagcg gatcgcggca
     6601 tcgtcgacgc cgacgtcgag gtccggggga accgggtctc cctcgtgatg gacacgcgca
     6661 cgctccccga ggcgaccccg ccggccagcg gctcggagtg ggaggcgtcc accctgggcg
     6721 tgggcgactc ccccaacctc cagtacgtgg actgcgcacc ggagagcacg cagcacatca
     6781 cctatcccgg cggggagctc gtctccttcg gcaacggccg cgggagctgc tgagcggcag
     6841 ccttaggggc ggttgcgacc gaacaggtcg tgcgcctcga cccactcctc ggccacgatc
     6901 gccgagccga gcgagagctc gccgcacagc accgtcgcgg ccacgatctc ggtgagcttg
     6961 cgcaccctgt ccttgccgta gcagccgagc atctcgaggc actcgcgctg ggtggcgagg
     7021 cctgtcccgc caccgtagct cgccacgatg agcgacggga tcgtgaccga gtagtagtag
     7081 tcgccgttcg gtcgcagctc cgcgtacacg aacgcggccg aggactccgc cacgttcgcc
     7141 gcgtcctgcc cagtggcgat gaacatcgcc gtgatcccgt tggccgagtg ggcgccgttg
     7201 ttgttcacgc ccgacatgaa cccgccgagg ttcgacacct gccgggcgcg gtacatcagc
     7261 gcgctgtccg agcgcatgat ccgctggaac agctcgttcg ggatcgtggc ctcggcgatc
     7321 acgcgcttgc cgcgcgtgtt cagcatgttc acctgcgagc tcttcttgtc ggtcgcgaag
     7381 ttcgactcga ggaagtagtg ctcgattccc gggtagttct cggtgatcca cttgcacgcc
     7441 gcctgggtcg ccttgccggt gaggttctgg cccgcggcgt cgcccgtcgt gtagttgaag
     7501 cgcgtgtaga ggatccgaga ggcggagtac tgctcgatgt cctgcagctt gcccgtcgag
     7561 gtcgtggact cggccacctc gcggatcgcg tcgaagttct cgtccagcca ctcgccgaac
     7621 gctcgcgcct c
//
LOCUS       DASVPL010000028        31444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_120246, whole genome shotgun sequence.
ACCESSION   DASVPL010000028 DASVPL010000000
VERSION     DASVPL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 31444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31444
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120246"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VF517_01410"
     CDS             <1..260
                     /locus_tag="VF517_01410"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX8051620.1"
                     /translation="RVAAVLCLAFPLEAPRRSGPPRSRLDELDAVTVPTLVVQGERDR
                     FGIPPPATNRRVTIVPGDHGLKKDVTAVAQTVANWLRDLAI"
     gene            320..529
                     /locus_tag="VF517_01415"
     CDS             320..529
                     /locus_tag="VF517_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEX8051621.1"
                     /translation="MDTKDIAVAALTGIGGVNWGLVALAEFDLVAKVTGNRFGTTNVA
                     SRVVYGLVGAAAAYQLARTAQAAKA"
     gene            complement(526..1545)
                     /locus_tag="VF517_01420"
     CDS             complement(526..1545)
                     /locus_tag="VF517_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8051622.1"
                     /translation="MPAERNLTVAVTGPTGTFGFGLVPLLQDDARVKRVVGIARRPFD
                     PAEHGWTKMEYRRGDVRERAALEEAFAGADVVVHLAFMITGTASRETIRAINVEGTLN
                     AFRAAAAAGARRFVYASSVAAYGFHPDNPVGMDESWPVRPAAHLFYAQEKAELEQLLG
                     EENERHPELDLYLLRPPIVLGPHAVGAKDILPAPVAALGRRLLELVARLPVPVPAPTP
                     DAPVQFIHEDDVGQALLRCVVAAGPPGAYNIAADGIMTGSDIARELGLSPVSIPAGVV
                     HAAAGAAAQLPKPPFAPPATDWVEAMSHPAVMDTSKAKQQLGWSPRYTAVEALRETLP
                     TSAGG"
     gene            complement(1610..2116)
                     /locus_tag="VF517_01425"
     CDS             complement(1610..2116)
                     /locus_tag="VF517_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051623.1"
                     /translation="MTAGFADELTATIAAFLVEIGIPVEPADLPDGPFLPGVALERGG
                     LLVDEPRLEYPGDLLHEAGHVAVAPAWARPSMSGAIDVEGLDTSDLEWAAIPWSYAAA
                     LAIGIDPAVVFHEGGYRGRSAGLLANFAVGVPIGAHLLVEAGMTASAPLAAELGVPPY
                     PHMLRWLR"
     gene            complement(2119..2757)
                     /locus_tag="VF517_01430"
     CDS             complement(2119..2757)
                     /locus_tag="VF517_01430"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX8051624.1"
                     /translation="MAAKRPLVLVLLLAALAGGCGEGDGGFGIVDAATADPNATDAAF
                     ARAIVPQEREALALARIARRRALRRELREIAKKMLAQPPERIRELGGIAADLEHDGVR
                     PAGAEMNRRPTGTSGLRDAVSFDFRFMELMIRHLERAVAMSEEEQDRGGHPRLKYLAG
                     ELMKTHERELDALRRWLRTWYGEGVLPGEGGGGGGGGEQPAPDGGAPADPPL"
     gene            complement(2810..3169)
                     /locus_tag="VF517_01435"
     CDS             complement(2810..3169)
                     /locus_tag="VF517_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8051625.1"
                     /translation="MPLDPRAPGIARELVRGLGVDDATRRTLELIVSELVTNSVVHGD
                     VAPEGELALRIKSDTDGVRGEVCGPGPTFEWEQHEPNLTTPGGLGLMLVDNLAESWGI
                     TRNGDTCVWFQCLSEKL"
     gene            3425..3658
                     /locus_tag="VF517_01440"
     CDS             3425..3658
                     /locus_tag="VF517_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051626.1"
                     /translation="MAKDHGPSVKNDKQYEGLRKKGMSKSRAAAISNSEGSESRGGKS
                     SGSSQSKKSSGSGSGGNKAQKAAAGRKGGKKSS"
     gene            3867..4361
                     /locus_tag="VF517_01445"
     CDS             3867..4361
                     /locus_tag="VF517_01445"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051627.1"
                     /translation="MKALTQSEIREVVAGVAEDEARWRPLVRHDPAQRLYEALDPGDA
                     EFEGRLGLWLICWMDGHDTGFHDHGVSSGTVRVVSGEVLDERPGPGGTIDARTYGAGE
                     SFDFGSWDIHRLSHAGRVPAVSIHAYSPPLERMNAYVVGPGGVLRPTEVTYGDELRPL
                     DAAA"
     gene            complement(4378..5646)
                     /locus_tag="VF517_01450"
     CDS             complement(4378..5646)
                     /locus_tag="VF517_01450"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8051628.1"
                     /translation="MHAARRQGQEQAPLALARPLTLRVRSAAAACALLACLLAPPSAL
                     AAPDCPGTAPPREVMSGLGVLEAIAADRAGRLYLSDMGGGRVLRVDRPGAEPHVLARF
                     PSPGGLTWDADGTLIAGYGNGAAQGVADNGQAGLNRVDPDSGETATIASGMGMANGVV
                     RGPDGAVYVTNTFGREGGRIDRVRGAQVDEAWSPVDGLNGLVIDRAGQNLYGSSTSGP
                     PRIARIPIARPAEVETYAAPTEADGLNGFDGMARDDADRLYVTTQPAEEVWRIDGPGR
                     ICALARGIEAPSAVAFGGGGAGFPADSLYVITFTGRVIEVPAATDRPPAPGPPLRLRL
                     AVEPRTVRAGRAARFRFRVRAADRGRTLPVAGAIVRFAGRRVATDGAGRAELVRRIVR
                     PRALPANATARGYQAARAQVRVRAPALRRR"
     gene            complement(5586..6338)
                     /locus_tag="VF517_01455"
     CDS             complement(5586..6338)
                     /locus_tag="VF517_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051629.1"
                     /translation="MWRGAIVVLMVAWACAWPGAAAAEPVSFAHGTIEHRYTTAQPNA
                     PTGFSFSGTYHAANDPSADPPYMRKMTFYSPAGLRYDTSVPDRCTATDVQLQVQGPAA
                     CPEGSRLGGGTARGKFMGSESKLDVDVFNNAGEQVMLIHSPLIATVSRGRIADDGSIE
                     YASPTCYPALVTCPTDTALQLGSDIVTQPYTRDGRSYTTTPPRCPKSGYWESPVRFWW
                     ADGSDETVVTKQPCTRPAAKAKSKRRSRSRAR"
     gene            complement(6532..7239)
                     /locus_tag="VF517_01460"
     CDS             complement(6532..7239)
                     /locus_tag="VF517_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051630.1"
                     /translation="MLSAAIVAGAVPGAAQAGPRFDYRQVFTTSAPGASTGIDTQILY
                     KHPDDPDAKPIPVRQEVFTFPVGTRFDESVVPDCTVSDLQLQLQGVSACPAATWLGSG
                     HGNTSMTGFPGAGENPVLVNAFDFGSDRFRVLGESEDLPLRFIAHGEGTGRTRTVDVP
                     ATPGGPPDGEGALRRVRNIFPPRSAGGRAAVRTPRKCPSSGTWTFKARLTFADGGVER
                     NVHRMPCRRRARRGTAR"
     gene            complement(7315..7749)
                     /locus_tag="VF517_01465"
     CDS             complement(7315..7749)
                     /locus_tag="VF517_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8051631.1"
                     /translation="MEADLPDLLPLMREYCTFYEAEPADERLLSLSRALLDDPRCEGF
                     QLIARDERGHALGFATVYWSWSTLAASRTAILNDLYVRPEARGSGLAEALIEEARVRA
                     GDRAASIGWQTAKDNLRAQRVYERVGARRSEWVDYSLDAAPS"
     gene            complement(7770..8240)
                     /locus_tag="VF517_01470"
     CDS             complement(7770..8240)
                     /locus_tag="VF517_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051632.1"
                     /translation="MPFPPRPIDSTLHGMTDYTVGALLMTAFPKLAGIEGSQAATQIR
                     VAGAIHAGYSTLTDYPLGAVKAIPYKAHLALDAIGALALAATPFVTGQWKKGTGQWVP
                     HLALAAFELGSLVMSDPTGNGNYHGDVKAVRAANMEDPARKIYEGPPAVTPAVD"
     gene            complement(8242..9432)
                     /locus_tag="VF517_01475"
     CDS             complement(8242..9432)
                     /locus_tag="VF517_01475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX8051633.1"
                     /translation="MRATVWSGRNTVQVEEVPDPKILNERDAIVKISSTAICGSDLHL
                     YDGYVPEMKRGDILGHEFMGEIVEKGKGVPNLEVGDRVVIPFPIACGNCWSCKHELYS
                     ACENSNPNSGIAEKMFGHSTCGIFGYSHITGGYAGGQAEYARVPYADVNQIKIEDDLT
                     DEQVLFLSDVFPTGYFGADLCDIKGGEVIAVFGAGPVGLFAIASAIMMGAERVIAIDQ
                     YDYRLQMARNRAGATDVINFSEDADIVEQLKELSGGRGPDAVIDAVGMEAVHGHGVLH
                     AVERQKQAMRAETDRGHALRDAILACRPGGIVSVMGVYGGLMDKFPTGAFLNKSLQLR
                     AGQAPVHKYMKRLYDHVRNGDIDPSFVVTHRLPLDEASNAYETFKHKQDDCVKVVLKP
                     GKDG"
     gene            complement(9432..9821)
                     /locus_tag="VF517_01480"
     CDS             complement(9432..9821)
                     /locus_tag="VF517_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051634.1"
                     /translation="MSSRAAITVMKPREELERLWSSSEYRPDYVDGTDAAVTFRDAPG
                     DRGTEIYVDLEGAPPGGKLGEMVAKLAGKDPRAKVMDDLRRFKQHVETGVIARSDGAP
                     EGELAERKLKQRPAQPLDDSEIEKAGV"
     gene            9933..12290
                     /locus_tag="VF517_01485"
     CDS             9933..12290
                     /locus_tag="VF517_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX8051635.1"
                     /translation="MSRAGACVAAILLVLAAAPAAQAFDTGPHHDMTVDALSAEGFAR
                     PAADIVAVNNYFVDLYSNEKENPFSGHHSWYHPLTIVGGGTFHNEHWDAALVEATDRL
                     HFDPHDQNQTSTTAEIEAEWERLQRGTTRSLKDAKDLWALSRGPSGYPQQAADAALTV
                     LSALGVSLHALQDFYTHTNWLEPGAGAGGQGPGWAAQGQGTTPTWYDLPKGLRDGADI
                     YAGRSPGHRPHGSWKEDRNENLNRGMNKDWSGRPLFTEAYRAAYFATRQWVRAVRRWL
                     ANEELWGRAMALAERPAELQRDVLGGAFLMSLYSGHWQGEGEPCNPKGGWPAPIGKGE
                     LSCGERSGWGGNLLDLRSATKSYFQRGKTRFRGFFEGAARAVGYHSPVGETFPVESSR
                     DIQAATRFVRLQVLSIRGVELGDPGPDDADMYTRASIAGQDFDSAVIHSHDRFSFPRP
                     YHAFTWLRSVPAGGSYGEPVSTMTVELRTSNDRSAGTDDDVSLRIGSGRTWTLDKRLY
                     DDFERGDRDTYSVPIDAATRRGLSVGDIQQVEIAKSKDGVGGGWKLAGVKLTVNGRVL
                     YRNDRVNRWLENSKRVWRAPDFAPSAPAGPALPVWLDLREDDTVYGGDDQGDVNPYDA
                     RDALARGYVPGTVAEQTISGGEKYRGRLGKDGDDGVLRYRIDTLDMVPPPPLPKPPDP
                     PPEDPPKPGDPPPPPKPDLIVTALNQYSFTVKNQGAGAAGPFTVTLVGAGTHNFDGLA
                     AGASASRTYNSSCEAGSSEARADSLDQVDESDETNNVTRGGPYFC"
     gene            complement(12304..12711)
                     /locus_tag="VF517_01490"
     CDS             complement(12304..12711)
                     /locus_tag="VF517_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051636.1"
                     /translation="MHDVALIVAGSLALLGAAIHGVGGQMLVVRNLSPETLPPSRFGG
                     PRTTMSMIHITWHMTTVGFVTVGVSLLLAGSVLDGDTARGVGVVAAAAATGFAAISIG
                     LGVANSRSARGLLRHPAPALLTTTALLAWLGSL"
     gene            12753..12959
                     /locus_tag="VF517_01495"
     CDS             12753..12959
                     /locus_tag="VF517_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051637.1"
                     /translation="MTLEQRAAAAAAEAKRAEEAGDRWAAHDAWRRYQRIVDAARPPG
                     ELVERANRLRRLAASVRVLDKPTR"
     gene            12956..13435
                     /locus_tag="VF517_01500"
     CDS             12956..13435
                     /locus_tag="VF517_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051638.1"
                     /translation="MIDTPFEVGRMLGRLNGSGIPYVVVGGIAVIVHGYVRPTEDLDV
                     LVPQRSPIGDALRVLLADWGATRLDGSALPDALFDGEHNVRARTPDGVVDFIPEGEPP
                     LDFEAVRDSAQRVEIDGVETRVCDLRHLAAFKRLAGRLRDRADLAELEAAHGALPDV"
     gene            complement(13432..15693)
                     /locus_tag="VF517_01505"
     CDS             complement(13432..15693)
                     /locus_tag="VF517_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8051639.1"
                     /translation="MLRGLRLSLCVVAVLAFMTATASAQASFDARGSAEQVDVTGLTP
                     GAEASLLDGAGRTVATREADAQGGLLFRKVKPGSGYRVRSGGTTSEPVTVLTNQAAPP
                     DPKVYDQQIPSSGYGYLTTRDGTKLAINVHPPQDITNASPAGFLPQVPTGPTPTLIEY
                     SGYGYANPAGPTNGIAIIANLMGFTVVDVNMRGTGCSGGAFDFFEPLQSLDGYDVVET
                     VARQSWVMHNKVGMMGISYGGISQLFTAQMQPPSLAAISPLSVLDGVQTTLYPGGVLN
                     TGFAVEWAKEREKEAKPAGPDAGQPWAYKRIQEGDQVCKDNQALHGQAADLQAKIRAN
                     NTYKPEIVDELSPITFVDKIKVPTFMACQWQDEQTGGHCPTLAARFTGTNRKWFTFTN
                     GTHVDSLAPETFNRWYDFLKLYVAKQAPATNSAHIRAGAPLIYQAAMGIDGQTLPPDP
                     IQDQPTYEGALAAFEQLDPVRVLFDNGAGGSPPGAPVPGFEHSFKSFPVPGTTARSWF
                     FSGKGALRGKPSARARASSFTWDAKARPLTDFTGDTAAGEDGLWTATPPYKWLEPPAG
                     TGASYVSSPLKATTTVVGAGAVRAWVRSSRKNVDLQATVTEVRPDGKETFVQSGWVRA
                     NRRKLDAKRSTPLAPVLSLRAKDVAAMPSNRFVKVTIPLYYQGHAYRAGSRIRVTITA
                     PNGDQPVWSFSETQPKGKATVAIAYSKRMPSSLTLPVVPDVAVPTELPPCPALRAQPC
                     RDYKPAANRTASR"
     gene            complement(15787..16248)
                     /locus_tag="VF517_01510"
     CDS             complement(15787..16248)
                     /locus_tag="VF517_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051640.1"
                     /translation="MSITKKFRAIALVAACIAALGASGTALAGSTRDADGDGIANVRD
                     RDIDGDSVRNGRDRNTDGDSLRNSRDRDVDADGARNSRDRDIDGDGQANSVDTDDDAD
                     GILDVDDPDADGDGVADAEEEQQREDCPDHGSRGDSSNDDSGSSAQPTPAT"
     gene            16363..16911
                     /locus_tag="VF517_01515"
     CDS             16363..16911
                     /locus_tag="VF517_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8051641.1"
                     /translation="MGSIHVHEFMSLDGVIDTPTWTFDYGFDPRMGEAIGGLTASSRG
                     ILLGRTTYEMFEPAWSSRTEEDDPGAPFFNDTTKYVVSSTLTEATWNNSEIVGPYDPD
                     AIRRLKDEVDGDLYVSGSGTLVRAMLADGLVDGLHLFVFPLTRGSGPRLFTEEDAPGK
                     LALTNTDTYPNGVVYLAYRPEV"
     gene            16983..17645
                     /locus_tag="VF517_01520"
     CDS             16983..17645
                     /locus_tag="VF517_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8051642.1"
                     /translation="MRLHDYGPSPNCHKVRILLAQLGRDYERVPVDIFGGDTLEPSYF
                     EKNPGLTTPVLEVEPGVYLPESGAILLYLAEGSEFLPDDPLERAHVHRWMMYEQARLF
                     GIVGTLRFFVMSGRLDPESQQARQQLRFSAAAIGLLEAHLASNEFFVAGRYTVADIAL
                     YGYLQVAHEAGVDMGAFPNVTAWLDRVREQPGHVADLEPYPDNARVGKGRSIYDLVTI
                     ES"
     gene            complement(17658..19073)
                     /locus_tag="VF517_01525"
     CDS             complement(17658..19073)
                     /locus_tag="VF517_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8051643.1"
                     /translation="MRALRAAALCAVCLAVLTGGARAQGLPAETIKARQHFFGAENVA
                     ADGTLPRGRVILSWFSVASFAAAIDGHAVLLDTYIHKGEDRPNYVPTTTDELAALRPE
                     AIFIGHGHYDHASTAGQLAARTNATVVGTPEHCDQTRAQAEAYAGHVLPIRCVEAVDR
                     GSAPGAEVRELRPLGDDVCVSVLKHVHSAAEPPDAEHRDPQLLPPPLIDPTLVLVHPP
                     GPSAVPGLANTAGDEGSSLLYQFRVGRFALVWNDSSGPLRERAPQVFDVMRRLPATDV
                     ELNAVVGFNNPTNGVRDPVDYIDAIRPKVMYPTHHDFVAEYGSGDDFEQYMYREMSRH
                     EGLTAATELRWLRDPYDYLRPGLMSFNVNDPRWASDDPARPEGACGCPRPRKSTFKLH
                     RVPGTRITRAELFVNGRRTLVRTGRDLRRVSAPLPATGRLKVRIVATHTSGARVVSTR
                     TYDGCTKTRPVIRVVRPPRRS"
     gene            complement(19084..19440)
                     /locus_tag="VF517_01530"
     CDS             complement(19084..19440)
                     /locus_tag="VF517_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051644.1"
                     /translation="MDEDGYLFIVDRKKDLIIRGGYNVYPREVEEVIYSHPAVREAAV
                     VGVPHDELGEEVGAAVAVRDGEELSPDDLRAYVKERVAAYKYPRVIWLVDELPKGPTG
                     KILKREIETPDEDGAT"
     gene            complement(19441..20580)
                     /locus_tag="VF517_01535"
     CDS             complement(19441..20580)
                     /locus_tag="VF517_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8051645.1"
                     /translation="MNFATLFSQSASEHADRQAVKLDDLALTYSMLDAGVARAAGLLR
                     SKGVGEGDRVAIQLPNVPYFPVLYFGALRIGAVLVPMNPLLKGREVSYYLNDAGVRLM
                     FAWPDFAEAAEAGAAEAGAECVITKPGEFEQLLGGAEPVEEVTDRADDEPAVIIYTSG
                     TTGAPKGATLTHANLSAGASTARELVEIGPEDVAVATLPLFHVFGMNSVMNVCLVHGG
                     LLTLVPRFGPEKVLEVIERDQATLFAGVPTMFSALLNHPGSKDTDTSSLKFCVSGGAA
                     IPVEVLRGFEDAFDATILEGYGLSETTGMGSFNLPDKRKPGSIGVPVGGTEMKVVDDD
                     DNDVPQGEPGEVVMRGPFVMREYWGKSEATDEVMRGGWFHTGDVA"
     gene            complement(20628..21644)
                     /locus_tag="VF517_01540"
     CDS             complement(20628..21644)
                     /locus_tag="VF517_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051646.1"
                     /translation="MPAQTDSATGAAAAAAELRRRAEAQGGSLIAMPRDVVALAGRKR
                     LSKRLRQDLEEAMTREGLVAEPRMTQATSFSIVIWTLASAPPGVLERQGLTRDGGGGH
                     TGAANGEHADDRAAFSAGLTQVLESQGFAIGLRAHDVKTEGAMKQGEHLVEAMTLLLG
                     GGVGPGLLSGGGAEASQVSEALVPFVPLSDVLALRFDAAGLAILVPVVFADELDANEV
                     GRRLTAFLELSPPLAEFGPRIYGTSSASCNTFPMVVYLDSARYAASVPSLLPLGFQKK
                     VMARAYLRAGFVDVPSGAVAWAERPGVWGWGNKLASALGKKPRDHFNFGPGELQAIAA
                     LTRR"
     gene            21751..22326
                     /locus_tag="VF517_01545"
     CDS             21751..22326
                     /locus_tag="VF517_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8051647.1"
                     /translation="MADQQRDVIEVLEHDHREVEEMFTELESLRGASTDEERTRRKEL
                     TEQVTIELVRHSVAEEVLVYPRVEAKVSAEEVEHAREEHAEAEETLQRLEKLDADASE
                     FDDELATLMEEIRHHIEDEEGEMFAHMRQVLDADELRDMGGAVEAFKKVAPTRPHPNV
                     PNEALPRLAVGPAASLFDRMRDLASGRGTGD"
     gene            complement(22362..22658)
                     /locus_tag="VF517_01550"
     CDS             complement(22362..22658)
                     /locus_tag="VF517_01550"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX8051648.1"
                     /translation="MQLFMIRRRTAWQTPEELGATAERSKQVAADEFPDDIRWIRSYV
                     VSESDGALGTVCIYEAANEQAVRDHAAKVDMPADEVLAVADTVVVRPDPEPATA"
     gene            22733..23395
                     /locus_tag="VF517_01555"
     CDS             22733..23395
                     /locus_tag="VF517_01555"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8051649.1"
                     /translation="MSGEVAARGAEAGFPRSRGRPSEGAREALVGAARELFTEHDFHA
                     VGTAQVLARAGVSRGAMYHHFPSKTDLFRAAWETSERDLLARFTERSTGGGSPFESLV
                     SGCRAYLVECATSRELQRIGLRQSRAVLGWEQWSEAVAALGLGVMKAGVGAAVESGEL
                     RTNDVDATARILLSALIEAGLLIATARDPRARLREIEPEAMRFVLGLGVAAVDSAGGG
                     DG"
     gene            23388..24770
                     /locus_tag="VF517_01560"
     CDS             23388..24770
                     /locus_tag="VF517_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8051650.1"
                     /translation="MADTDTRIGSGTRVAEPRASRRPPARGRPSSEVPGPRLPGLLQT
                     LLGVALPLEARLAMRKRYGRVFRTDDAIAGEMFHIADRDLVEQIFKWKPAQYNVGEPR
                     QLMEPVTGPTSVLLLDGAPHMRMRKLMLPPFHGEAIAHYAELIEQVTGREIDAWRPGD
                     TVRMRTVAQRITMEVIIRAVFGVTDPGRLAELQRVLPRLSSPSPFLLLVQKDLGPRSP
                     WGRFLRLRDHVDRLIYEEIAERRGEGDGGTRKDILTLLLSARDEAGDPLSDRELRDEL
                     ITLLLAGHETTATSIGWAFERLLRTPAALERLTAEVAAGESGEYLDAVVKETMRVRPV
                     VPEVFRSPTERTELGGYVFEPGAHLTAAILLVQLDPELYPPDPEAFRPERFLDGAPEP
                     YTWIPFGGGVRRCLGAAFATLEMEVVIATVLARTRLRASRSRGEAARFRGVTMLPSRG
                     GEAVVDRVLA"
     gene            24809..25855
                     /locus_tag="VF517_01565"
     CDS             24809..25855
                     /locus_tag="VF517_01565"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX8051651.1"
                     /translation="MRRQLLDPLGEVDALEVVRRLCGVQAQVPSAAALAVRVRQASPS
                     SDGVSRALEDRRLMRTWAMRGTLHLLAPDEAGAYLALAGAARSWEKGSWQKAYGLTPD
                     EMALLVEAVGEALDGRVLGRDELVAAVCERLGRPELEAALRSGWGSVLKPVAWQGGLC
                     HATSDGNRVRFARPDQWLADWTGVPAPEDAAQVVIPAYLRAYGPATPERFDAWLTRGS
                     SRRAALRSWFASLEDRLATVEVGGEPALLLADDVEELGATAPTQVVRLLPGFDQYVLG
                     PGTRAEEIIAPSHRADVSRAAGWISPVVVAGGRVVGTWEADGRTVRVRLFEDAGPVAG
                     DALDAEVARAEALL"
     gene            complement(25919..26389)
                     /locus_tag="VF517_01570"
     CDS             complement(25919..26389)
                     /locus_tag="VF517_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051652.1"
                     /translation="MNPFVRITVSVNALPAHMRHQDGEDIVNPSGPCGAAVAAKALPA
                     GAAPAADAAPTDAAPAPAPVVSNGTGASPGTAQAPASVSSPAEGNGVRGVAARSEDRS
                     PAGTTASRDRAKRSSGSSGSLPFTGFAVLFLVLIGTGAVLAGTKLRWSTRDQHQ"
     gene            complement(27020..27568)
                     /locus_tag="VF517_01575"
     CDS             complement(27020..27568)
                     /locus_tag="VF517_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8051653.1"
                     /translation="MGRTRDGDPADFADFYERNAALAYGLAIRIVGRERAEDVTQEAF
                     LTVWRSRGQYTAELSGARSWLLTIVRRRAIDALRREGARPRTLVLEGAETVGTDRLRG
                     AETGSPAVAVVERDEARVVRHALTSIPPDQRQALELAYFNGLAHSEIAERLGLPLGTV
                     KGRIRHGLVKLHDRLSDAGVCR"
     gene            27766..28551
                     /locus_tag="VF517_01580"
     CDS             27766..28551
                     /locus_tag="VF517_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051654.1"
                     /translation="MSEPVNNVYEAVEAAYSDRPAATRRQLVGGAAATVGSLTVLGAL
                     PELASAEHTNEPQTVLNVAATAEVLATIVNTVGREKVKLDAVTDANVAAAAREELIHE
                     RVLEASGGKPVTTQIHVPDEVFANATNLLSTLEVGDQIFINAYLLATTVFATQRYRNP
                     KLARYTAEFMGVEAVHRALARQSLGKLGNDRAFMRYVQSEEADAPNRGAGGFKRIETA
                     VAQLEAAGFGFGKAGSKPGQMYDFAEVSKRTPNPSGVNTTSPR"
     gene            complement(28608..29219)
                     /locus_tag="VF517_01585"
     CDS             complement(28608..29219)
                     /locus_tag="VF517_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX8051655.1"
                     /translation="MTADLRALALNCTLKPSPAESSTEALAQVVLDALAGLGATTEMI
                     RVVDHDVRPGVTSDEGGGDQWPLIHEKLLAADILVLASPTWLGQPSSIAQRVLERMDA
                     MLAETDELGRPVAYDRVAGIVVTGNEDGAHHVIANLGQALIDIGYTIPGQAWTYWNKG
                     PGPGEEVYLTSDDREWSDATGRTAAANLAAVARALRSKPMPAP"
     gene            complement(29248..29667)
                     /gene="msrB"
                     /locus_tag="VF517_01590"
     CDS             complement(29248..29667)
                     /gene="msrB"
                     /locus_tag="VF517_01590"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8051656.1"
                     /translation="MTARIEKSDQDWREELTPAQYEVLRRKGTERPFSGGYVHTEDDG
                     MYRCAGCGTALFSSDTKFDSGTGWPSFTEPAVAANIELHTDRSFLMKRTEVTCAACGG
                     HLGHVFDDGPREAGGKRYCINSAALALDPDTDTDGAS"
     gene            complement(29721..30320)
                     /locus_tag="VF517_01595"
     CDS             complement(29721..30320)
                     /locus_tag="VF517_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8051657.1"
                     /translation="MTRAPEVGTAQDPDAVLVQQASAGDMAAFEQLVMRHADALHGAL
                     LRFGLDHGEAQDVAQETFLRAWRSLSRFEARSRFFTWLYRIGFNEAQRRLAKRPAAGA
                     VVSTEDRPLDDLAAEGPGPVGTLEREELRATLAEGLRELPADLRAPVVLRDVEGLSTS
                     EAASVLDLGEAAFKSRLHRGRMALRAMLAPRLSGLPGFG"
     gene            complement(30304..30579)
                     /locus_tag="VF517_01600"
     CDS             complement(30304..30579)
                     /locus_tag="VF517_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX8051658.1"
                     /translation="MRNPFTALSFARDHAWSQQHMSEYIDAELGARGAGRIERHTDDC
                     PECAELLASLRMMVATLAALPGRPGQSVAAAVLAGVHDRLADDDSRA"
     gene            30689..>31444
                     /locus_tag="VF517_01605"
     CDS             30689..>31444
                     /locus_tag="VF517_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8051659.1"
                     /translation="MTSASAVGPLARLADVRTRTLELVEPLSDSDAEAVHSPLMSPLA
                     WDLGHIAAFEDLWLVHRAAGEPLLRPGLAEIYDALETPRAHRGELQFLGRSAALDYLA
                     EVRGRVEELAPSDVLLQELVIQHELQHNETMLQTLALARLDGYVPPGYVSPADQRLPQ
                     VEQRTPTGLETVVVPPGAFELGAPAGRFSYDNERPRHTTDVPAFEIGRVPVTNGAFGE
                     WIAAGGYARREWWSEAGWEWRRASGVERPGSWFD"
BASE COUNT         4294 a        11247 c        11458 g         4445 t
ORIGIN      
        1 cccgcgtcgc cgcggtgctc tgcctcgcgt tcccgctcga ggcgccgcgc cggtcgggtc
       61 cgccgcggag ccgcctcgac gagctcgacg ccgtgaccgt ccccacgctc gtcgtgcagg
      121 gcgagcgtga ccgcttcggc atcccgccgc cggccacgaa ccgccgcgtg acgatcgtgc
      181 cgggcgacca cgggctcaag aaggacgtca ccgccgtcgc gcagacggtc gcgaactggc
      241 tccgcgacct cgctatctga cgatctccgc gccccgctga gacgtactcc cggaaacgaa
      301 ggcaacaagg gagcagggaa tggacaccaa ggacatcgca gtcgcggcgc tgaccggcat
      361 cgggggcgtc aactggggcc tggtcgcgct ggccgagttc gacctcgtcg cgaaggtcac
      421 cggcaaccgc ttcggcacga ccaacgtcgc cagccgggtc gtctacgggc tcgtgggtgc
      481 ggccgcggcc taccagctcg cgcgcaccgc gcaggcggcg aaggcctagc cgccggcgct
      541 cgtcggcagc gtctcgcgca gcgcctcgac ggccgtgtag cgcgggctcc agccgagctg
      601 ctgcttggcc ttcgacgtgt ccatgaccgc cgggtggctc atcgcctcga cccaatcggt
      661 cgcgggcggt gcgaacggcg gcttcggaag ctgagcggcg gcgccggccg cggcgtgcac
      721 cacgccggcg gggatcgaga cgggcgacag gccgagctcg cgggcgatgt cgctgccggt
      781 catgatcccg tcggccgcga tgttgtaggc gccgggcggc cccgcggcca ccacgcacct
      841 gagcagcgcc tggccgacgt cgtcctcgtg gatgaactgc acgggcgcgt cgggcgtggg
      901 ggcggggacc ggcaccggca gccgcgcgac gagctcgagc aggcgccgcc ccagcgccgc
      961 cacgggcgcg gggaggatgt ccttcgcgcc cacggcgtgc gggcccagca cgatcggcgg
     1021 gcgcagcagg tacaggtcga gctccggatg cctctcgttc tcctcgccga ggagctgctc
     1081 cagctcggcc ttctcctgcg cgtagaagag gtgcgcggcg ggccggacgg gccagctctc
     1141 gtccatgccg accgggttgt cggggtggaa gccgtacgcc gccaccgacg aggcgtagac
     1201 gaaccgcctg gccccggcgg cggccgcggc ccggaacgcg ttcagcgtgc cctccacgtt
     1261 gatcgcgcgg atcgtctcgc gcgacgccgt gccggtgatc atgaacgcga ggtgcaccac
     1321 cacgtcggcg ccggcgaacg cctcctcgag cgcggcacgc tcgcgcacgt cgccgcggcg
     1381 gtactccatc ttcgtccagc cgtgctcggc cgggtcgaac gggcgccggg cgatcccgac
     1441 cacgcgcttc acgcgcgcgt cgtcctggag cagtgggacg aggccgaacc cgaacgtccc
     1501 ggtcgggccg gtgacggcga cggtcaggtt gcgctcggcg ggcatgcgag gccggtaccc
     1561 cgttcgcgac ctcgcgaacc cgccgccccg tcggacggtc gccgagcacc tagcgcagcc
     1621 agcgcagcat gtgcgggtac ggcggcacgc cgagctcggc ggcgagaggc gccgacgcgg
     1681 tcatccccgc ctcgacgagc aggtgcgctc cgatcgggac gcccaccgcg aagttggcga
     1741 gcaggcccgc cgaacgcccg cggtagcccc cctcgtggaa caccacggcg gggtcgatcc
     1801 cgatggcgag cgcggcggcg taggaccagg ggatcgccgc ccattcgagg tcgctcgtgt
     1861 cgagaccctc gacgtcgatc gcgccgctca tcgagggccg ggcccaggcg ggcgcgaccg
     1921 ccacgtgccc ggcctcgtgc agcaggtcgc ccggatactc gaggcgcggc tcgtccacca
     1981 gcagcccacc gcgctccagc gccacgccgg gcaggaacgg cccgtccggc aggtcggccg
     2041 gctcgacggg gatgccgatc tccaccagga aggcggcgat cgtcgcggtc agctcgtcgg
     2101 cgaagccggc cgtcatcgct agagcggggg atccgcgggc gcgccgccgt cgggcgccgg
     2161 ctgctcgccg ccgcctccac cgccgccgcc ctcgccgggc agcacgccct cgccgtacca
     2221 cgtccgcagc cagcgcctga gcgcgtcgag ctcgcgctcg tgcgtcttca tcagctcgcc
     2281 cgcgaggtac ttgaggcgcg ggtggccgcc gcggtcctgt tcctcctccg acatcgccac
     2341 cgcacgctcg aggtggcgga tcatcagctc catgaagcgg aaatcgaacg agacggcgtc
     2401 gcgcagtcca ctcgtgccgg tcgggcgccg gttcatctcg gcgccggccg gacgcacccc
     2461 gtcgtgctcg aggtcggccg cgatcccgcc gagctcgcgg atccgctccg gcggctgcgc
     2521 cagcatcttc ttggcgatct cgcgcagctc gcgccgcagc gcccgcctgc gggcgatccg
     2581 cgccaacgcc agcgcctccc gctcctgcgg cacgatcgcg cgggcgaagg ccgcgtcggt
     2641 ggcgttcggg tcggcggtcg ccgcgtcaac gatcccgaag ccgccgtcgc cctcgccgca
     2701 gccgccggcg agcgcggcca gcagcaggac gagcacgagc gggcgtttag cggccacgcc
     2761 cggatcgtag gcgcgggcga ctccccgcgc gaacggcgca gctagcgcgc tagagcttct
     2821 cgctcaggca ctggaaccag acgcacgtgt cgccgttgcg cgtgatgccc cagctctcgg
     2881 cgaggttgtc cacgagcatc aggccgagcc cgccgggcgt ggtcaggttg ggctcgtgct
     2941 gctcccactc gaacgtcgga cccggcccgc agacctcgcc gcgcaccccg tcggtgtcgg
     3001 acttgatccg cagggcgagc tcgccctcgg gcgcgacgtc gccgtgcacc acggaattcg
     3061 tcaccagctc gctcacgatc agctcgaggg tccggcgcgt agcgtcgtcg acccccagcc
     3121 cgcgcaccag ctcacgcgcg attccgggag ccctcgggtc gagaggcaat gtgtcttcga
     3181 ttagagcgct cacgcctgac cgttacccgg agcaagcgcg tcgaagcgtc tcgcgggaca
     3241 tgttggcccg cggcaacccg attttccgga gagagcgaaa cgactccagc ggccggcgaa
     3301 cccgttagac ggcaccgagc gagtcgccgc atcgccctca ccgcacgggt acccgccgcg
     3361 gtggtgccta accatggatg gcgggtaatg gatagatgac ggcaacgcag cggaggaggc
     3421 gacgatggcg aaggaccacg gacccagcgt caagaacgac aagcagtacg agggcctgcg
     3481 gaagaagggc atgagcaagt cgcgcgcggc ggcgatctcg aactcggagg ggagcgagag
     3541 ccgcggcggc aagagctccg gcagcagtca gagcaagaag agcagcggct cgggcagcgg
     3601 cggcaacaag gcgcagaagg cggccgccgg gcgcaagggc ggcaagaagt cgagctagcc
     3661 gctcggctgg cgcggaaggc ggcggcgcca ccgattagcc gcccggcgcg gcgggtaatc
     3721 caagcgtacg cccaagcctc acatgggggg agggcgccgc tgtagcgact gggaagcgtg
     3781 cgggccgcag tgccgcgggc tgaccttcgg ctggtgacca ttcgtcgtcc gaaggggaag
     3841 cgcatgccgc actcgcaggg gccgcgatga aggccctgac ccagtccgag atccgtgagg
     3901 tcgtcgccgg cgtggcggag gacgaggcgc gctggcgccc gctcgttcgc cacgaccccg
     3961 cccagcgcct gtacgaggcg ctcgatccgg gcgacgccga gttcgagggc aggctcggcc
     4021 tctggctgat ctgctggatg gacggccacg acaccggctt tcacgaccac ggcgtctcgt
     4081 cgggcacggt ccgggtcgtg tcgggggagg tgctcgacga gcgcccgggg cccgggggca
     4141 cgatcgacgc gcgcacgtac ggcgccgggg agtcgttcga cttcggttcg tgggacatcc
     4201 accgcctgtc tcacgcgggt cgcgtgccgg cggtctcgat ccacgcctac tcgcccccgc
     4261 tcgagcggat gaacgcctac gtcgtcgggc ccggcggcgt cctgcggccg acggaggtca
     4321 cctacggcga cgagctgcgt ccgctcgacg cggcggcgta gcgatgtcgc gcgtcggcta
     4381 gcggcgccgc agcgccgggg cgcgcacccg cacctgcgcg cgagcggcct ggtagccgcg
     4441 cgcggtcgcg ttcgccggca gcgcgcgcgg gcgcacgatg cggcggacca gctcggcgcg
     4501 ccctgcgcca tccgtggcga cccgccgtcc ggcgaagcgc acgatcgcgc cggccacggg
     4561 gagcgtccgg ccgcggtcgg cggcgcgcac ccggaaccgg aagcgcgcgg cccggccggc
     4621 ccggaccgtg cgcggctcga ccgccaggcg cagccgcagg ggcgggcccg gagccggggg
     4681 gcggtcggtc gccgcgggca cctcgatcac ccgtcccgtg aacgtgatga cgtagaggct
     4741 gtccgcgggg aagccggcgc cgccgccgcc gaacgcgacc gcgctcggcg cctcgatccc
     4801 gcgcgccagc gcgcagatcc gcccggggcc gtcgatccgc cagacctcct ccgccggctg
     4861 ggtggtcacg tacaggcggt cggcgtcgtc gcgtgccatg ccgtcgaagc cgttcaaccc
     4921 gtcggcctcg gtcggggccg cgtaggtctc gacctccgcc ggccgggcga tcgggatgcg
     4981 ggcgatgcgc ggcggcccgc tggtgctcga gccgtacagg ttctgacccg cgcggtcgat
     5041 cacgaggccg ttcagcccgt ccaccggcga ccaggcctcg tcgacctgcg cgccgcgcac
     5101 gcggtcgatc cggcctccct cgcgtccgaa cgtgttcgtc acgtacacgg cgccgtcggg
     5161 cccgcgcacg accccgttcg ccatgcccat gcccgatgcg atcgtcgcgg tctccccgct
     5221 gtcggggtcg accctgttga gccccgcctg gccgttgtcg gcgacaccct gcgcggcacc
     5281 gttgccgtag ccagcgatca gggtgccgtc cgcgtcccag gtgaggcctc ccggggaggg
     5341 gaagcgcgcg agcacgtgtg gctcggcgcc gggacggtcg acgcgcagca cccgcccgcc
     5401 gcccatgtcg gacaggtaaa ggcggccggc gcggtcggcg gcgatcgcct cgagcacgcc
     5461 gagcccgctc atgacctcgc gcggcggggc cgtgccgggg cagtcgggcg ccgcgagcgc
     5521 ggacggcggg gcgagcaggc aggcgagcag cgcgcacgcc gccgcggcgc tccggacgcg
     5581 cagggtcagc gggcgcgcga gcgcgagcgg cgcttgctct tggccttggc ggcgggccgc
     5641 gtgcatggct gcttggtgac cacggtctcg tccgagccgt ccgcccacca gaagcggacc
     5701 ggtgactccc agtagccgga cttcgggcac ctgggcggcg tggtcgtgta gctgcgcccg
     5761 tcgcgcgtgt agggctgcgt gacgatgtcc gagccgagct ggagcgcggt gtcggtgggg
     5821 caggtgacga gtgccgggta gcaggtgggc gacgcgtact cgatcgagcc gtcgtcggcg
     5881 atccgcccgc ggctcacggt ggcgatcagc ggcgaatgga tcagcatgac ctgctcgccc
     5941 gcgttgttga acacgtcgac gtcgagcttc gactcggacc ccatgaactt gccgcgcgcg
     6001 gtcccgccgc cgagccggct gccctccgga cacgccgccg gcccctgcac ctggagctgg
     6061 acgtcggtcg cggtgcaacg atccgggacg ctcgtgtcgt agcgcagccc ggcgggcgag
     6121 tagaaggtca tcttgcgcat gtacggcggg tcggcgctcg ggtcgttcgc ggcgtggtag
     6181 gtgccgctga agctgaagcc ggtcggagcg ttcggctggg cggtcgtgta ccggtgctcg
     6241 atcgtcccgt gggcgaagct gaccggctcg gccgcggccg cgccgggcca cgcgcacgcc
     6301 cacgcgacca tgagtacgac gatcgccccc ctccacatgg gggggttgtt ccccaacggg
     6361 cgcgtcgcca acctaaatcc cgagtgcggt tcgcgtggcg gcgcgcaggc acgtgatcgc
     6421 gcgacaaagc gtgctcgcct ggatgcgccg ggtcttcggc ggcgcgttcc tcgcgctggc
     6481 cggcaggctc gccctcgccg agcgctgagg gtccgggggc gcggccgcct actagcgcgc
     6541 ggtgccgcgc cgagcacggc gcctgcacgg catccggtga acgttgcgct cgaccccgcc
     6601 gtcggcgaac gtgaggcgcg ccttgaaggt ccacgtgccc gacgacgggc actttcgcgg
     6661 cgtgcgcacc gcggcacggc cgcccgccga ccgcggcggg aagatgttgc ggacccgccg
     6721 cagggcgccc tccccgtcgg ggggcccgcc gggcgtcgcc ggcacgtcca cggtccgggt
     6781 gcggccggtg ccctccccgt gcgcgatgaa ccgcagcggg aggtcctcgg actcgccgag
     6841 cacgcggaac cggtcagagc cgaagtcgaa cgcgttgacg agcaccgggt tctcgcccgc
     6901 acccgggaac ccggtcatcg acgtgttgcc gtgcccgctc cccaaccagg tcgctgccgg
     6961 gcacgccgac acgccctgga gctggagctg caggtcggac acggtgcagt ccgggacgac
     7021 ggactcgtcg aaccgcgtgc ccaccgggaa cgtgaagacc tcctggcgga ccgggatcgg
     7081 cttcgcgtcc gggtcgtcgg ggtgcttgta gaggatctgg gtgtcgatcc cggtggacgc
     7141 gcccggcgcg ctcgtggtga agacctgccg gtagtcgaac cggggccccg cctgtgcggc
     7201 gcccgggacc gcgccggcga cgatcgcggc gctcagcaca gcgatcgctc tccccctcga
     7261 cgacgtcccc cgagccatct ccacgaaggc tacgtgctcg ggcgcctccc cgcgtcagct
     7321 cggtgcggcg tccagcgagt agtcgaccca ctccgagcgc cgcgcgccga cccgctcgta
     7381 cacccgctgg gcgcgcaggt tgtccttcgc ggtctgccag ccgatcgacg cggcgcggtc
     7441 cccggccctc acgcgcgcct cctcgatcag cgcctcggcg aggcccgacc cgcgcgcctc
     7501 ggggcgcacg tacaggtcgt tcaggatcgc ggtccgcgag gcggcgagcg tcgaccagct
     7561 ccagtagacg gtggcgaagc cgagggcgtg tccgcgctcg tcgcgcgcga tgagctggaa
     7621 gccctcgcag cgcgggtcgt ccagcagggc gcgcgacagg gacagcagcc gctcgtcggc
     7681 cggctcggcc tcgtagaacg tgcagtactc gcgcatcagc gggaggaggt ccggcaggtc
     7741 ggcctccacc acgggtgaga cgtgcgggct cagtcgaccg cgggagtgac cgccggcggg
     7801 ccctcgtaga tcttgcgcgc cgggtcctcc atgttggccg cccgcacagc cttcacgtcg
     7861 ccgtggtagt tgccgttgcc ggtcgggtcg ctcatcacca gcgagcctag ctcgaaggcg
     7921 gccagcgcca ggtgcggcac ccactggccg gtgcccttct tccactggcc ggtgacgaac
     7981 ggagtcgcgg ccagcgccag tgccccgatc gcgtcgagcg ccaggtgcgc cttgtacggg
     8041 atcgccttga cggcgccgag cgggtagtcg gtcagcgtgc tatagccggc gtggatcgca
     8101 ccggcgaccc tgatctgcgt ggcggcctgc gagccctcga tgccggcgag cttcgggaac
     8161 gcggtcatca gcagcgcgcc gacggtgtag tcggtcatgc cgtgcagcgt cgagtcgatc
     8221 gggcgcggtg ggaagggcac gtcaaccgtc ctttccgggc ttgagcacga ccttcacgca
     8281 gtcgtcctgc ttgtgcttga acgtctcgta ggcattcgac gcctcgtcga gcgggagccg
     8341 gtgggtgacc acgaagctcg ggtcgatgtc gccgttgcgc acgtggtcgt agagcctctt
     8401 catgtacttg tggacgggtg cctggcccgc ccgcagctgg aggctcttgt tgaggaacgc
     8461 gccggtgggg aacttgtcca tgagcccgcc gtagacgccc atcaccgaca cgatgccgcc
     8521 cggccggcag gccaggatcg cgtcgcgcag ggcgtggccg cgatcggtct cggcgcgcat
     8581 cgcctgcttc tggcgctcga ccgcgtgcag tacgccgtgg ccgtgcaccg cctccatccc
     8641 gacggcgtcg atcaccgcgt cggggccgcg gccgccggac agctccttga gctgctcgac
     8701 gatgtcagcg tcctcggaga agttgatgac gtcggtcgcg cccgccctgt tccgggccat
     8761 ctggagccgg tagtcgtact ggtcgatcgc gatcacccgc tcggcgccca tcatgatcgc
     8821 gctcgcgatg gcgaacaggc cgaccggccc ggccccgaag accgcgatca cctcgccgcc
     8881 cttgatgtcg cacaggtcgg ctccgaagta gcccgtcggg aagacgtccg acaggaacag
     8941 gacctgctcg tcggtgaggt cgtcctcgat cttgatctgg ttgacgtcgg cgtacgggac
     9001 ccgcgcgtac tcggcctgac cgcccgcgta gccgccggtg atgtgcgagt agccgaagat
     9061 gccgcaggtc gagtgcccga acatcttctc ggcgatgccg gagttcgggt tcgagttctc
     9121 gcaggcggag tacagctcgt gcttgcacga ccagcagttg ccgcaggcga tcgggaacgg
     9181 aatgaccacg cggtcgccga cctcgaggtt cgggacgccc ttccccttct cgacgatctc
     9241 gcccatgaac tcgtggccga ggatgtcgcc gcgcttcatc tccgggacgt agccgtcgta
     9301 gaggtggagg tcggagccgc agatcgcggt ggagctgatc ttcacgatcg cgtcgcgctc
     9361 gttgaggatc ttggggtccg ggacctcctc gacctggacc gtgttgcgtc ctgaccagac
     9421 ggtcgcgcgc atcagacccc cgccttctcg atctcggagt cgtccagcgg ctgagcgggc
     9481 cgctgcttga gcttgcgctc ggccagctcg ccctccggcg cgccatcgga gcgggcgatc
     9541 acaccggtct cgacgtgctg cttgaagcgg cgcaggtcgt ccatcacctt cgcgcgcggg
     9601 tccttgccgg cgagcttggc caccatctcg ccgagcttgc cgccgggagg ggcgccctcg
     9661 aggtccacgt agatctcggt gccgcgatcg cccggcgcgt cccggaaggt cacggccgca
     9721 tcggtgccgt cgacgtagtc ggggcggtac tcggagctgc tccagagccg ctccagctcc
     9781 tcgcgcggct tcatcaccgt gatcgctgct ctcgaactca cgctctgcct cctcgaacca
     9841 tcgattccgt ggggggaaag ctgcccgatg gggccaaccg gaaaccgcgg cggcggcttg
     9901 acttggcgcg tgcgccggat cagactcggc cggtgtcccg cgcgggtgcc tgcgtggccg
     9961 cgatcctgct ggtcctggcc gccgcccccg cggcgcaggc gttcgacacg ggtccgcacc
    10021 acgacatgac cgtcgacgcc ctcagcgccg agggcttcgc gcggccggcg gccgacatcg
    10081 tggcggtcaa caactacttc gtggacctgt actcgaacga gaaggagaac ccgttctccg
    10141 ggcaccactc ctggtaccac ccgctcacga tcgtcggcgg gggcacgttc cacaacgagc
    10201 actgggacgc cgccctggtg gaggcgacgg accgcctgca cttcgacccc cacgaccaga
    10261 accagacctc caccaccgcc gagatcgagg ctgagtggga acgcctccag cgcggcacca
    10321 cgcgctcact caaggacgcg aaggacctgt gggcgctctc gcgggggccg tctggctacc
    10381 cgcagcaggc ggccgacgcc gcgctgaccg tgctcagcgc gctcggggtg agcctgcacg
    10441 cgctccagga cttctacacg cacacgaact ggctcgagcc cggcgccggg gccggcggcc
    10501 agggtccggg ctgggcggcc cagggccagg gcaccacgcc gacctggtac gacctcccca
    10561 aggggctccg cgacggcgcc gacatctacg ccgggcgctc gccgggccac cgcccgcacg
    10621 gaagctggaa ggaggaccgc aacgagaacc tcaacagggg gatgaacaag gactggtcgg
    10681 gtcgccccct gttcacggag gcgtaccgag ccgcgtactt cgccacccgc cagtgggtgc
    10741 gcgcggtgcg gcgctggctc gcgaacgagg agctgtgggg gcgtgcgatg gcgctcgcgg
    10801 agcggccggc ggagctccag cgcgacgtcc tcggcggagc gttcctgatg tcgctctact
    10861 cgggccactg gcagggcgag ggcgagccgt gcaaccccaa gggcggctgg ccggcaccga
    10921 tcggcaaggg cgagctgagc tgcggcgagc ggagtggctg gggcgggaac ctgctcgacc
    10981 tccggagtgc gaccaagtcg tacttccagc gcggcaagac ccgcttccgc ggtttcttcg
    11041 agggcgccgc cagggcggtg ggctaccaca gcccggtcgg cgagacgttc ccggtcgagt
    11101 cgagccgcga catccaggcc gccacgcgat tcgtccggct ccaggtgctg tcgatccgcg
    11161 gcgtcgagct cggcgacccg ggcccggacg acgccgacat gtacacgcgc gcgagcatcg
    11221 cggggcagga cttcgactcg gccgtgatcc acagccacga ccggttcagc ttcccgcggc
    11281 cctaccacgc gttcacgtgg cttcggtcgg tgccggccgg cggcagctac ggagagccgg
    11341 tgtcgacgat gaccgtcgag ctgcgcacct cgaatgaccg cagcgccggg accgacgacg
    11401 acgtctcgct gcggatcggc tccggccgta cctggacgct cgacaagcgc ctctacgacg
    11461 acttcgagcg cggcgaccgc gacacctaca gcgtgccgat cgacgccgcc acgcggcgcg
    11521 gcctgagcgt gggcgacatc cagcaggtgg agatcgccaa gtcgaaggac ggcgtgggag
    11581 gaggctggaa gctcgccggt gtgaagctga cggtcaacgg gcgcgtgctg taccgcaacg
    11641 accgcgtgaa ccgctggctc gagaacagca agcgcgtgtg gcgggctccg gacttcgcgc
    11701 cctcggcccc ggccggcccg gccctgccgg tctggctcga cctgcgcgag gacgacaccg
    11761 tctacggcgg cgacgaccag ggcgacgtga acccctacga cgcgcgcgac gcgctcgcgc
    11821 gcgggtacgt gccaggcacg gtcgccgagc agacgatcag cggcggcgag aagtacaggg
    11881 gccggctcgg caaggacggc gacgacgggg tcctgcgcta ccggatcgac accctcgaca
    11941 tggtgccgcc tccaccgctg ccgaagccac cggacccgcc gccggaggac ccgccgaagc
    12001 cgggcgaccc accgccaccg ccgaagcccg acctgatcgt caccgcgctg aaccagtact
    12061 cgttcacggt gaagaaccag ggcgccggcg cggctggtcc gttcaccgtc accctcgtgg
    12121 gcgccgggac gcacaacttc gacgggctgg cggcgggggc gtcggccagc cggacctaca
    12181 acagcagctg cgaggccgga agcagcgagg cccgcgccga ctcactcgac caggtcgacg
    12241 agagcgacga gacgaacaac gtgactcgcg gcggtccgta cttctgctga gctcgccctc
    12301 gcgctagagc gaccccaacc acgcgagcag tgcggtcgtg gtgaggagtg ccggggcggg
    12361 gtggcgcagc aggccccgcg cggatcgcga gttcgcgacg ccgagcccga tgctgatcgc
    12421 cgcgaagccc gtcgccgccg cggcggccac cacgccgacc ccgcgcgccg tgtcgccgtc
    12481 gaggaccgag cccgcgagca ggagcgagac acccacggtg acgaagccga ccgtggtcat
    12541 gtgccacgtg atgtggatca tgctcatggt cgtccgcggg ccgccgaagc gactcggcgg
    12601 cagcgtctcc ggcgacaggt tccgcaccac gagcatctga ccgcccacgc cgtggatcgc
    12661 ggcgccgagg agcgcgagcg acccggcgac gatcagcgcg acatcgtgca cgcccgcacc
    12721 gtaccgctag ccggctcctc tacgatctgg gcatgacgct cgagcagcgc gcggcggccg
    12781 ccgcggcgga ggctaagcga gctgaggagg cgggcgaccg ctgggccgcg cacgacgcgt
    12841 ggcggcgcta ccagcgcatc gtcgacgccg cacgaccgcc aggtgagctc gtcgagcgag
    12901 cgaaccggct gcggcgactt gccgccagcg tgcgggtgct cgacaaaccc acgcggtgat
    12961 cgacacgccc ttcgaggtgg gccgtatgct cggccgccta aatggctccg ggatccccta
    13021 tgtcgtggtc ggtggtatcg cggtgatcgt ccacggctat gtccgcccca ccgaggatct
    13081 cgatgtactg gtcccacaac gttcaccgat aggggatgcg ctgcgggtac tcctggcgga
    13141 ctggggcgcg acgcggctcg acggctcagc ccttcccgac gcgctgttcg atggcgagca
    13201 caatgtgcgc gctcggacgc ccgatggcgt cgtcgacttc atccccgagg gcgagccgcc
    13261 gctcgacttc gaagcggtcc gggactctgc tcagcgggtt gagatcgacg gcgtggagac
    13321 gcgcgtgtgc gaccttcgcc acctggcggc gttcaagcga ctcgccggaa ggcttcgcga
    13381 ccgcgccgac ctggccgagc tcgaggccgc gcacggggcg ctacccgacg tctagcgcga
    13441 cgccgtgcgg ttcgcggccg gcttgtagtc gcgacacggc tgggccctga gggcggggca
    13501 cggcggcagc tcggtcggca cggcgacgtc gggcacgacc gggagcgtca ggctcgacgg
    13561 catgcgcttc gagtacgcga tcgccaccgt cgccttcccc ttcggctgcg tctcgctgaa
    13621 cgaccagacg ggctggtcgc cgttcggcgc cgtgatcgtc acgcggatgc gcgatccggc
    13681 gcggtaggcg tgaccctggt agtagagcgg gatcgtgacc ttcacgaagc ggttgctcgg
    13741 catcgccgcg acgtccttgg cgcggaggct gagcacgggc gcgagcggcg tgctccgctt
    13801 ggcgtccagc ttgcgcctgt tcgcgcgcac ccagccgctc tgcacgaacg tctccttgcc
    13861 gtccggcctc acctcggtga ccgtggcctg gagatcgacg ttcttgcgcg acgaccgcac
    13921 ccaggcgcgc accgctccgg cgccgacgac ggtcgtggtg gccttcagcg ggctcgacac
    13981 gtacgacgcg ccggtcccgg ccggaggctc gagccacttg tagggcgggg tggcggtcca
    14041 cagcccgtcc tcgcccgcgg cggtgtcgcc ggtgaagtcc gtcagcggac gggccttggc
    14101 gtcccacgtg aacgagctcg cacgcgcgcg cgccgacggc ttgccgcgca gcgccccctt
    14161 gcccgagaag aaccacgagc gcgcggtcgt gcccggcacg gggaagctct tgaacgaatg
    14221 ctcgaagccc gggaccggcg cgcccggagg ggagccgccg gcgccgttgt cgaacaggac
    14281 gcggaccggg tcgagctgct cgaacgcggc gagcgccccc tcgtaggtcg gctggtcctg
    14341 gatcgggtcg ggcggcagcg tctgtccgtc gatgcccatc gccgcctggt agatcagcgg
    14401 cgcccccgcg cggatgtgcg cggagttcgt ggcgggcgcc tgtttggcca cgtacagctt
    14461 caggaagtcg taccagcggt tgaacgtctc gggcgcgagc gagtcgacgt gcgtcccgtt
    14521 ggtgaaggtg aaccacttgc ggttggtccc ggtgaagcgc gcggccagcg tcgggcagtg
    14581 cccgccggtc tgctcgtcct gccactggca ggccatgaac gtcgggacct tgatcttgtc
    14641 gacgaaggtg atcggcgaca gctcgtcgac gatctcgggc ttgtaggtgt tgttcgccct
    14701 gatcttcgcc tgaaggtccg ccgcctgccc gtggagggcc tggttgtcct tgcagacctg
    14761 atcgccctcc tggatccgct tgtacgccca cggctgcccg gcgtcgggcc ccgccggctt
    14821 ggcctccttc tcccgctcct tcgcccactc caccgcgaag cccgtgttga ggacgccgcc
    14881 cggatagagc gtcgtctgca cgccgtcgag gaccgacagc ggcgagatcg cggccaggct
    14941 cgggggctgc atctgcgccg tgaagagctg gctgatcccg ccgtaggaga tgcccatcat
    15001 cccgaccttg ttgtgcatca cccacgactg gcgcgcgacc gtctcgacca cgtcgtagcc
    15061 gtcgaggctc tgtagcggct cgaagaagtc gaacgcgccg cccgagcagc cggtgccgcg
    15121 catgttcacg tccaccaccg tgaatcccat gaggttcgcg atgatcgcga tgccgttcgt
    15181 cgggccggcc gggttcgcgt agccgtagcc cgagtactcg atcagggtgg gggtggggcc
    15241 ggtcgggacc tgggggagga agcccgcggg cgaggcgttc gtgatgtcct gcggcgggtg
    15301 cacgttgatc gcgagcttgg tgccgtcgcg cgtggtcagg tagccgtagc cgctcgacgg
    15361 gatctgctgg tcgtagacct tcggatccgg cggcgccgcc tgattcgtga ggaccgtgac
    15421 cggctccgac gtcgtaccgc cgctcctcac gcggtagccg gagcccggct tgaccttgcg
    15481 gaagagcagg ccgccctgtg cgtcggcctc ccgtgtggcg accgtgcggc cggcgccgtc
    15541 gagcagagac gcctccgcgc ccggcgtcag gccggtcacg tcgacctgct cggcgctgcc
    15601 gcgcgcgtcg aacgacgcct gggccgacgc cgtcgcggtc atgaacgcga ggacggcgac
    15661 gacgcaaagc gacaggcgga ggccccgaag cacggcgaca actcaagcag gacggggcgc
    15721 ggagtgtcgc agtcgcggca cgaccgggcg ggcgaggggg ccgcccgccc ggtcgctgcg
    15781 atcgcctcag gtggcggggg tcggctgggc cgagctgccc gagtcgtcgt tcgagctgtc
    15841 gccgcggctg ccgtggtccg ggcaatcctc gcgctgctgc tcctcctcgg cgtcggcgac
    15901 gccgtcgccg tccgcgtccg gatcgtcgac gtcgaggatc ccgtcggcgt cgtcgtcggt
    15961 gtcgacagag ttggcctggc cgtcaccgtc gatgtcgcgg tcgcgcgagt tgcgggcgcc
    16021 gtcggcgtcc acgtcgcggt cgcgcgagtt gcgcagcgag tcgccgtcgg tgttgcggtc
    16081 gcgcccgttg cggacgctgt cgccgtcgat gtcgcgatcg cggacgttcg cgatcccgtc
    16141 gccgtcggcg tcgcgtgtcg agccggccag cgccgtaccc gacgcgccca gtgcggcgat
    16201 gcacgccgcg accagggcga tggctcggaa cttcttggtg atgctcatga gatggctcct
    16261 ctcgtggggt atttgcacga gagacgtgcg gggcgcgccg gtgtttggcc gagcgtgtgt
    16321 tttcttaggt tgctcttcga ctgaacgaca cggaggtacg cgatgggatc catacacgtg
    16381 cacgagttca tgtccctcga cggcgtcata gacacgccga cgtggacgtt cgactacggc
    16441 ttcgatcccc ggatggggga ggcgatcggc ggcctcacag cgagctcgcg gggcatcctg
    16501 ctcgggcgga cgacctatga gatgttcgag cccgcgtggt cgagccggac cgaggaggac
    16561 gaccccgggg cgccgttctt caacgacacg accaagtacg tcgtctcgtc cacgctcacc
    16621 gaggcgacgt ggaacaactc ggagatcgtc ggaccgtacg accccgacgc gatccgccgg
    16681 ctgaaggacg aggtcgacgg cgacctctac gtgagcggca gcggcacgct ggtccgggcg
    16741 atgctcgccg acggcctcgt cgacgggctg cacctgttcg tcttcccact cacccgcggg
    16801 tccggcccgc ggctgttcac cgaggaggac gcgcccggca agctggcgct gaccaacacc
    16861 gacacgtacc cgaacggcgt ggtctacctc gcgtatcgac ccgaggtgtg agggcccggc
    16921 aggctagcgg tgggtgagat cgggcacgcc gggcggatcg gcggcggcgg atactgcccg
    16981 gcatgcgcct acacgactac ggaccatcgc ccaactgcca caaggttcga atcctcctcg
    17041 cccagctcgg ccgcgactac gagcgggtac cggtcgacat cttcggcggc gacacgctcg
    17101 agcccagcta cttcgagaag aacccggggc tcacgacgcc cgtgctcgag gtggagcccg
    17161 gcgtctacct ccccgagtcc ggagcgatcc tgctctacct ggccgagggg agcgagttcc
    17221 ttcccgacga tccgctcgag cgcgcgcacg tgcaccgctg gatgatgtac gagcaggcgc
    17281 ggctgttcgg gatcgtgggc acgctgcgct tcttcgtgat gagcggccgg ctcgatcccg
    17341 agagccagca ggcgaggcag cagctccgct tctcggcggc ggccatcggc ctgctggagg
    17401 cgcacctcgc gagcaacgag ttcttcgtgg ccggccgcta cacggtcgcc gacatcgcgc
    17461 tgtacgggta cctccaggtc gcgcacgagg cgggcgtgga catgggcgcg tttccgaatg
    17521 tcaccgcctg gctcgaccgg gtccgcgagc agccgggcca cgtggccgac ctcgagccct
    17581 acccggacaa cgcgcgggtc ggcaagggcc ggtcgatcta cgacctggtg acgatcgagt
    17641 cgtagcgcgc cggccgttca ggagcggcgc ggcgggcgca ccacgcggat gaccggcctg
    17701 gtcttcgtgc acccgtcgta ggtgcgggtg gagaccacgc gcgcgccgct cgtgtgcgtc
    17761 gcgacgatcc gcaccttcag cctgccggtc gccgggagcg gcgcgctcac cctgcgcagg
    17821 tcgcgaccgg tgcgtaccag cgtccgcctc ccgtttacga acagctcggc ccgcgtgatg
    17881 cgcgtgccgg gaacgcggtg gagcttgaag gtcgacttgc gcggccgcgg gcagccgcac
    17941 gcgccttccg gccgcgccgg gtcgtcgctc gcccagcgcg ggtcgttcac gttgaagctc
    18001 atgagcccgg ggcgcaggta gtcgtaggga tcgcgcagcc agcgcagctc ggtcgcggcg
    18061 gtcaggccct cgtgccgtga catctcgcgg tacatgtact gctcgaagtc gtcgcctgac
    18121 ccgtactcgg cgacgaagtc gtggtgcgtc ggatacatga ccttcggccg gatcgcgtcg
    18181 atgtaatcca ccgggtcgcg cacgccgttc gtcgggttgt tgaagccgac caccgcgttc
    18241 agctcgacgt cggtcgcggg caacctgcgc atcacgtcga agacctgcgg cgcgcgctcg
    18301 cgcagcgggc ccgagctgtc gttccagacg agcgcgaagc gtccgacgcg gaactggtag
    18361 agcaggctcg agccctcgtc gcccgccgtg ttcgcgaggc ccggtaccgc ggacgggccc
    18421 ggcgggtgga ccaggaccag cgtcggatcg atcagcggcg gcgggaggag ctgcgggtcg
    18481 cggtgctcgg cgtccggcgg ctcggccgcg ctgtgcacgt gcttgaggac cgagacgcag
    18541 acgtcgtcgc cgagcggccg cagctcgcgc acctcggcgc cgggcgcgga gccgcgatcg
    18601 accgcctcga cgcacctgat cggcaggaca tggccggcgt acgcctccgc ctgcgcccgg
    18661 gtctggtcgc agtgctcggg cgtgccgacg accgtcgcgt tcgtgcgcgc ggcgagctgc
    18721 ccggcggtcg acgcgtggtc gtagtgcccg tgaccgatga agatcgcctc gggccgcagc
    18781 gccgcgagct cgtcggtggt ggtgggcacg tagttgggac ggtcctcgcc cttgtggatg
    18841 taggtgtcga gcaggacggc gtggccgtcg atggccgccg cgaagctggc gacgctgaac
    18901 cacgagagga tgacccggcc gcgtggaagc gtgccgtccg cggcgacgtt ctcggcgccg
    18961 aagaagtgct gcctcgcctt gatcgtctcg gccgggagtc cctgagcccg ggcgccgccg
    19021 gtcagcacgg caaggcacac ggcgcacagc gctgccgcgc gaagcgccct catcggctcg
    19081 gcgctacgtc gccccgtcct cgtccggcgt ctcgatctcc cgcttgagga tcttcccggt
    19141 cggtcccttc gggagctcgt cgacgagcca gatcacgcgc gggtacttgt aggcagcgac
    19201 ccgctccttc acatacgcgc ggaggtcgtc cggcgagagc tcctcgccgt cgcgcaccgc
    19261 cacggcggcg cccacctcct caccgagctc gtcgtgcggg acgccgacca cggccgcctc
    19321 gcgcaccgcg gggtgcgagt agatgacctc ctcgacctca cgcgggtaga cgttgtagcc
    19381 gccgcggatg atcaggtcct tcttgcggtc gacgatgaag aggtagccgt cctcgtccac
    19441 ctacgcgacg tcgccggtgt ggaaccagcc gccgcgcatc acctcgtcgg tcgcctcgga
    19501 cttgccccag tactcgcgca tgacgaacgg cccgcgcatg acgacctcgc ccggctcgcc
    19561 ctgcggcacg tcgttgtcgt cgtcgtccac gaccttcatc tcggtcccgc cgaccgggac
    19621 gccgatcgac ccgggcttgc gcttgtccgg gaggttgaac gagcccatgc cggtggtctc
    19681 cgacagcccg tagccctcga ggatcgtcgc atcgaaggcg tcctcgaacc ctcgcagcac
    19741 ctcgaccggg atcgccgccc cgcccgagac gcagaacttg agcgagctcg tgtccgtgtc
    19801 cttgctcccg gggtggttca gcagcgcgga gaacatcgtc ggcacgcccg cgaacagcgt
    19861 ggcctggtcg cgctcgatca cctcgagcac cttttccgga ccgaagcgcg ggaccagcgt
    19921 gagcaggccg ccgtggacga ggcagacgtt catgacgctg ttcatgccga acacgtggaa
    19981 gagcggaagc gtggcgaccg ccacgtcctc gggtccgatc tccacgagct cgcgggcggt
    20041 ggaggcgccc gccgagaggt tcgcgtgcgt cagcgtcgcg cccttcggcg cgcccgtcgt
    20101 gccggacgtg tagatgatca cggcgggctc gtcgtcggcg cggtcggtga cctcctcgac
    20161 cggctccgcg ccgccgagca gctgctcgaa ctcgccgggc ttcgtgatga cgcactcggc
    20221 gccggcctcc gccgcgccgg cctcggcggc ctcggcgaag tccggccagg cgaacatgag
    20281 gcgcacgccc gcgtcgttca ggtagtagct gacctcgcgc cccttgagca gcgggttcat
    20341 cggcacgagg accgcgccga tgcgcagcgc gccgaagtag aggaccggga agtacgggac
    20401 gttcgggagc tggatcgcga cgcgatcgcc ctcgccgacg cccttcgagc gcagcagccc
    20461 ggccgcgcgc gcgacgcccg cgtcgagcat cgaatacgtg agggcgaggt cgtcgagctt
    20521 gacggcctgg cggtcggcgt gttcgcttgc ggactgcgag aacagtgtgg cgaagttcat
    20581 gcggctcccc tgactaggac actcggcggc acatgatgac gcgcagctca gcgccgcgtg
    20641 agcgcggcga tcgcctggag ctcgcccggc ccgaagttga agtggtcgcg cggtttcttg
    20701 ccgagggccg acgcgagctt gttgccccag ccccagacgc ccggacgttc cgcccaggcg
    20761 accgcgccgg agggcacgtc cacgaacccc gcgcgcaggt aggcgcgggc catcaccttc
    20821 ttctggaacc cgagcgggag gagggagggc acgctcgcgg cgtagcgcgc ggagtcgagg
    20881 tagacgacca tcgggaaggt gttgcagctc gcgctgctgg tgccgtagat gcgcgggcca
    20941 aactcggcga gcggcgggga gagctcgagg aacgcggtca gccggcgccc gacctcgttg
    21001 gcgtcgagct cgtcggcgaa gacgaccggg acgaggatgg cgaggccggc cgcgtcgaag
    21061 cgcagtgcga gcacgtcgct cagcgggacg aacgggacga gcgcctcgct cacctggctc
    21121 gcctcggcgc caccgcccga gagcagcccg gggccgaccc cgcctccgag cagcagggtc
    21181 atcgcctcga cgagatgctc gccctgcttc atcgcgcctt cggtcttcac gtcgtgggcg
    21241 cggaggccga tcgcgaatcc ctggctctcg agcacctgcg tcagcccggc ggagaacgcc
    21301 gcgcggtcgt cggcgtgctc cccgttcgcg gcgccggtgt ggccgccgcc cccgtcgcgg
    21361 gtgagaccct gccgctcgag caccccgggc ggtgcgctcg ccagcgtcca gatgacgatg
    21421 gagaagctcg tggcctgggt catcctcggc tccgcgacca atccctcccg cgtcatcgcc
    21481 tcctccaggt cctggcgcaa gcgcttcgac agccgcttgc gccccgccag ggccaccacg
    21541 tcgcgcggca tcgcgatgag cgacccgccc tgggcctccg cgcggcggcg gagctcggcg
    21601 gcggccgccg cggctccggt cgcgctgtcg gtctgggctg gcactcggcg cgcgagtcta
    21661 aggccgtccc cgtgttcgcg atccgccggg cgggtagggc cgatggagcc gctgcgcgcg
    21721 gccccgggca aactcttcga ggaggcagcg atggccgacc agcagcgtga cgtgatcgag
    21781 gtcctggagc acgaccatcg cgaggtggag gagatgttca cggagctcga gtcgctgcgc
    21841 ggcgcctcca ccgacgagga gcgcacgcgg cgcaaggagc tgaccgagca ggtcacgatc
    21901 gagctcgtcc ggcactcggt cgccgaggag gtgctcgtct acccgcgcgt ggaggccaag
    21961 gtgagcgccg aggaggtcga gcacgcgcgc gaggagcacg cggaggcgga ggaaacgctg
    22021 cagcggctcg agaagctgga cgcggacgct tcggagttcg acgacgagct cgccacgttg
    22081 atggaggaga tccgccacca catcgaggac gaggagggcg agatgttcgc ccacatgcga
    22141 caggtcctcg acgccgacga gctgcgcgac atgggcggcg ccgtcgaggc cttcaagaag
    22201 gtcgcgccga ccaggccgca cccgaacgtt ccgaacgagg cgctgccgcg actcgcggtg
    22261 gggcccgcgg catcgctgtt cgaccgcatg cgggacctgg cgagcggacg cggcacaggc
    22321 gactgagccg cggcgctcgc ctcactcccc gctcgacccg gctaggccgt cgccggctcc
    22381 gggtccgggc gcacgacgac cgtgtcggcg acggccagca cctcgtcggc cggcatgtcg
    22441 accttcgcgg cgtgatcgcg gacggcctgc tcgttcgctg cctcgtagat gcagacggtc
    22501 ccgagcgcgc cgtcgctctc gctcacgacg tagctgcgga tccagcggat gtcgtcgggg
    22561 aactcgtcgg ccgcgacctg cttcgaccgc tcggccgtcg cgccgagctc ctccggcgtc
    22621 tgccacgcgg tgcgccggcg gatcatgaag agctgcatgt gtcctcctcc gaggtggttg
    22681 ccgggcgcca ggctacagga tttaccgaca ggctgtcggt atagtcgcag ggatgtcagg
    22741 tgaggtggcg gcgaggggag cggaggccgg gttcccccgc agtcgcggcc gaccgagcga
    22801 gggtgcccga gaggcgctgg tcggggccgc gcgcgagctg ttcaccgagc acgatttcca
    22861 cgccgtgggc accgcgcagg tgctggcacg ggcgggtgtg agccgcggcg cgatgtacca
    22921 ccacttcccc tcgaagaccg acctgtttcg agccgcgtgg gagacgagcg agcgcgacct
    22981 gctcgcgcgc ttcaccgagc gctcgaccgg tggggggagc cccttcgagt cgctcgtgag
    23041 cggctgccgc gcatatctcg tcgagtgcgc gacctcgcgc gagctccagc gcatcggtct
    23101 tcgccagagc cgcgcggtcc tcggctggga gcagtggagc gaggcggtcg ccgcgctcgg
    23161 gctcggggtg atgaaggccg gcgtcggcgc ggcggtcgag tcgggcgagc tccggacgaa
    23221 cgacgtcgac gcgaccgctc ggatactgct gtccgcgctc atcgaggcgg gcctgctgat
    23281 cgccaccgcg cgggacccgc gcgcgcgcct gcgcgagatc gagccggagg cgatgcgctt
    23341 cgtgctgggc ctcggcgtgg cggccgtaga ctccgccggg ggtggggatg gctgataccg
    23401 atacgcgcat cgggtccggg actcgcgtcg ccgagccccg cgcgagccgc cgcccgccgg
    23461 cccgcgggag gccgtcgtcc gaggtcccgg ggccgcgcct gccgggcctg ctccagacgc
    23521 ttctcggcgt cgcgctcccg ctcgaggccc gccttgcaat gcgcaagcgg tacggacgcg
    23581 tcttccgaac cgacgacgcg atcgcgggcg agatgttcca catagccgat cgcgacctgg
    23641 tcgagcagat cttcaagtgg aagccggcgc agtacaacgt cggtgagcca cggcagctga
    23701 tggagccggt gaccgggccg acatcggtcc tgctgctcga cggcgcgccg cacatgcgga
    23761 tgcgcaagct gatgctcccg ccgttccacg gcgaggcgat cgcgcactac gccgagctga
    23821 tcgagcaggt caccggacgc gagatcgacg cgtggcggcc gggggacacg gttcgcatgc
    23881 gcacggtcgc gcagaggatc accatggagg tgatcatccg ggcggtcttc ggcgtcaccg
    23941 atcccgggcg cctggcggag ctccagcgcg tgctgccgcg gctgtcgtcg ccgagcccgt
    24001 tcctgctgct cgtgcagaag gacctcgggc cgcgcagccc ctggggccgg ttcctccgcc
    24061 tgcgcgacca cgtcgatcgg ctcatctacg aggagatcgc agagcgccgc ggcgaggggg
    24121 acggcggcac ccgcaaggac atcctcacgc tgctcctatc ggcccgcgac gaggcgggcg
    24181 acccgcttag cgatcgcgag ctgcgcgacg agctgatcac gctgctgctg gccgggcacg
    24241 agaccaccgc gacttcgatc ggatgggcgt tcgagcggct gctgcgcacg cccgcggcgc
    24301 tcgagcgcct caccgccgag gtcgcggccg gcgagtccgg ggagtacctc gacgccgtcg
    24361 tcaaggagac gatgagggtc aggccggtcg tgcccgaggt gttccggtct ccgacggagc
    24421 gaaccgagct gggcggctac gtgttcgagc cgggcgccca cctcacggcg gcgatcctgc
    24481 tggtccagct cgaccccgag ctgtacccgc ccgaccccga ggcgttccgt cccgagcggt
    24541 tcctcgacgg ggcgccggag ccctacacct ggatcccgtt cggcggcggg gtgcgccgct
    24601 gcctgggggc ggcgttcgcg acgctcgaga tggaggtcgt gatcgcgacg gtgctggcgc
    24661 ggacccgcct gcgagcctcg cgctccaggg gcgaggcggc tcggttccgc ggcgtgacca
    24721 tgctgcccag ccgcggcggc gaggcggtcg tcgatcgcgt gctggcctga ggctgcagac
    24781 cacctggccg caggtactcg cgtggcggat gcggcgtcag ctgctcgacc cgctgggcga
    24841 ggtcgacgcc ctcgaggtcg tccggcggct gtgcggcgtc caggctcagg tgccgtcggc
    24901 ggcagccctg gccgtgcggg tgcgccaggc gagtccgtct tccgatgggg tgagccgggc
    24961 tctggaggac cgccggctca tgcggacgtg ggcgatgcgc ggcaccctcc acctgctcgc
    25021 gccggacgag gcgggcgcct acctggcgct ggccggagcg gcgcggtcgt gggagaaggg
    25081 gagctggcag aaggcgtacg gcttgacccc ggacgagatg gcactgctcg tcgaggccgt
    25141 gggcgaggcc ctcgacggcc gggtgctcgg ccgcgacgag ctggtggccg cggtgtgcga
    25201 gcgcctgggt cgcccggagc tggaggccgc cctgcgctcg ggctggggct ccgtgctgaa
    25261 gccggtcgcc tggcagggcg ggctgtgcca cgccacgtcc gacggcaacc gcgtccgctt
    25321 cgctcggccc gaccagtggc tcgcggactg gaccggggtg cccgcgccgg aggacgcggc
    25381 ccaggtcgtg atccccgcgt acctccgggc ctacgggccg gccacgccgg agcggttcga
    25441 cgcatggctg acgcgcggga gctccaggag ggccgccctg cggagctggt tcgcgagctt
    25501 ggaggaccgg ctggcgacgg tcgaggtcgg cggcgagccg gccctgctgc tggccgacga
    25561 cgtggaggag ctcggcgcca ccgcgccgac gcaggtggtc cggcttctcc cgggattcga
    25621 ccagtacgtg ctcgggccgg gcaccagggc ggaggagatc atcgcgccgt cgcaccgagc
    25681 cgacgtgagc cgagcggcgg gctggatctc gccggtcgtg gtcgccgggg gccgggtggt
    25741 cgggacctgg gaggccgacg ggcggacggt ccgggtgcgc ttgttcgagg acgctggtcc
    25801 ggtcgcgggg gatgcgttgg acgccgaggt ggcccgcgcc gaggcgttgc tgtgacgcgg
    25861 ggctgagcgc cgaagccggg agggcgaccc ggcggccgcc ctcccagcgc tcggtcggct
    25921 actggtgctg gtcccgcgtc gaccagcgga gtttcgtgcc cgccaggacg gcacccgtcc
    25981 cgatcagcac gaggaacagg accgcgaagc cggtgaacgg cagcgatccc gacgacccgg
    26041 acgaccgctt cgcgcgatcg cgcgaagcgg tggtaccggc gggtgaccga tcctccgagc
    26101 gcgcggccac gccgcgaacg ccgttgccct cggccggcga gctcaccgac gcaggagcct
    26161 gcgccgtgcc gggcgaggcc cccgtgccgt tgctcacgac gggcgcgggc gccggggcag
    26221 cgtcggtcgg ggccgcgtca gccgccgggg ccgcgccagc cggcagggcc ttggcggcaa
    26281 ccgccgctcc gcaggggccc gacgggttga cgatgtcctc gccgtcctgg tgccgcatgt
    26341 gcgcgggcag cgcgttgacc gagaccgtga tccgcacgaa cgggttcatc gccgagcccg
    26401 tcgcgtggca gatcgtgacc ttgccgtccg ggccaccggc gcgcgacggc gcggagtcgc
    26461 cccggccgtg gtcgtgccgg ctcgagcggt gctccgtccc accggagccg cggctcctcg
    26521 agccgccggt gccgtggtgt ctcgagccat ggccgttcga gcggccacgc gaccctcgac
    26581 tgggctgggc cggggtgccg cgggagtggc cgcgatgcga actgcctcgc gagctcccac
    26641 gtgagggcgc ctgtgcgctc ttgccccgcg agtggcccct gccgccacgc gcgtgctgcg
    26701 cgttcgagcc cccggaccca tgcccgggcg cggcggcccc gttgccgtgc gagttggacc
    26761 tgccctcgcc ggcgagggct gtccctgcca ctaccgccat ccatgccgcc acgatgatca
    26821 gccagatcga aaggcgagtc cctggggacc ttctgtccac gtctgcctcc ttgcgtgaga
    26881 cgtcgttgtg gtcggaggaa cgcccgccgt gcggagtggt cgaggagttc tcacgcgcca
    26941 cgcgtggttg cagacccttt ggctccggac ccagcgcagg cgcgccaggt tcacgggccg
    27001 agcaagcgtc gggtcgccgt cagcgacaca cgcccgcatc actcaggcgg tcgtgaagct
    27061 ttaccaaccc atgcctgatg cgccccttga ccgtgccgag cggaagcccc aggcgttcgg
    27121 cgatctcgct gtgggcgagc ccgttgaagt acgcgagctc gagcgcctgg cgctggtcgg
    27181 gtggtatcga ggtcagcgcg tggcgtacga cgcgagcctc gtcgcgctcg acgacggcta
    27241 ccgcgggcga gcccgtctca gcgccgcgta ggcgatccgt gcccaccgtc tcagcgcctt
    27301 cgagaacgag cgtccgcggg cgcgcgccct cccggcgcag cgcgtcgatc gcccgcctgc
    27361 gcacgatcgt cagcagccac gaccgggcac cgctcaactc agccgtgtac tggcctcggc
    27421 tccgccacac cgtcaagaag gcttcctgcg tcacgtcctc cgcccgctcg cggcccacga
    27481 tccggatcgc gaggccgtac gcgagcgccg cgttgcgctc gtagaagtcg gcgaagtcag
    27541 ccggatcacc atcgcgcgtt ctccccatcc gtcggttccc ctacgccccg gacgacccat
    27601 cggatcacat atggggcccc cacctgcggc gggcggctcc gggtggggtg cgcccgctgg
    27661 ctcgtcgcgc caattcccga gcgcgcctga tccggactcc cgtgcgctgc ggatacgcgt
    27721 cttcgagcag cacgcgctgc acgtccgaca acgggagagt ccgcgatgag cgagccagtc
    27781 aacaacgtgt acgaagcggt cgaggccgcc tactcagatc gtccggcggc cacgcggcga
    27841 cagctcgtcg gcggcgcggc cgccacggtg ggcagcctga cggtgctcgg cgcgcttccc
    27901 gagcttgcct cggccgagca caccaacgag ccccagaccg tgctcaacgt cgccgcgacc
    27961 gccgaggtgc tcgcgacgat cgtcaacacg gttggccgcg agaaggtgaa gctggacgcc
    28021 gtcacggacg cgaacgtggc agccgcggcg cgtgaggagc tgatccacga gcgcgtgctc
    28081 gaggcgtccg gcggcaagcc ggtcaccacg cagatccacg tgccggacga ggtgttcgcg
    28141 aacgcgacca acctgctctc gacactcgag gtcggcgatc agatcttcat caacgcgtac
    28201 ctgctcgcga cgacggtctt cgccacccag cgctaccgca acccgaagct cgctcgctac
    28261 accgcggagt tcatgggcgt cgaggcggtc catcgcgccc tcgcccggca gtccctgggc
    28321 aagctcggaa acgatcgcgc cttcatgcgc tacgtgcagt cggaggaggc cgatgctccc
    28381 aaccgcgggg cgggtggctt caagcggatc gagaccgccg tcgcgcagct cgaggccgcc
    28441 ggtttcgggt tcggcaaggc gggttcgaag cccggccaga tgtacgactt cgccgaggtc
    28501 agcaagcgca cgccgaatcc cagcggcgtg aacacgacct cgccgcgcta ggtccggcga
    28561 cctgagcggc cggcggcagc gagctttgcg gcgagctgcg ccggccgcta cggcgcgggc
    28621 atcggcttcg accgaagtgc ccgcgcgacc gcggcgaggt tcgcggcggc cgtccgcccg
    28681 gtggcgtcgg accattcgcg gtcgtcgctt gtgaggtaga cctcctcgcc ggggcccggg
    28741 cccttgttcc agtaggtcca cgcctggccg ggaatcgtgt acccgatgtc gatcagggcc
    28801 tggccgaggt tggcgatcac gtgatgagcg ccgtcctcgt tgccggtcac gacgatgccc
    28861 gcgacccggt cgtacgcgac gggccggccc agctcgtcgg tctccgcgag catcgcgtcc
    28921 atccgctcca gcacgcgctg cgcgatgctc gacggctgac ccagccaggt gggggaggcg
    28981 agcacgagga tgtcggcggc cagcagcttc tcgtggatca gcggccactg gtcgccgccg
    29041 ccctcgtccg acgtcactcc ggggcgcacg tcgtggtcca ccacgcggat catctccgtg
    29101 gtcgccccga ggcccgcgag tgcgtcgagc accacctggg cgagggcctc ggtgctcgac
    29161 tcggcgggcg agggcttgag cgtgcagttg agcgcgaggg cgcgcaggtc cgccgtcacc
    29221 gggaggggtc ccgcctcgtg agcgcgttca cgaggcgccg tcggtgtcgg tgtcggggtc
    29281 gagcgcgagc gcggccgagt tgatgcagta gcgcttgccg ccggcctcgc ggggcccgtc
    29341 gtcgaacacg tgcccgagat ggccaccgca ggccgcgcag gtcacctcgg tgcgcttcat
    29401 gaggaagctc cggtccgtgt gcagctcgat gttcgccgcc acggcgggct cggtgaagct
    29461 cggccagccg gtgcccgagt cgaacttcgt gtccgaagag aacagcgccg tgccgcagcc
    29521 cgcgcagcgg tacatgccgt cgtcctccgt gtgcacatag ccgccgctga acgggcgttc
    29581 cgtccccttc cggcggagca cctcgtactg cgcgggcgtc agctcctcgc gccagtcctg
    29641 gtcgctcttc tcgatgcgtg cggtcatcct cgctcctctg ccggttgctc gacgtatgac
    29701 ccgtgcgccg cgaaaacggt tcatccgaac ccggggagcc cgctgagccg gggcgccagc
    29761 atcgcccgga gagccattcg cccgcggtgg aggcgactct tgaacgccgc ctccccgaga
    29821 tcgaggaccg acgcggcctc cgaggtcgag aggccctcga cgtcgcgcag cacgaccggt
    29881 gcgcgcaggt ccgcgggcag ctcgcgcagg ccctcggcga gggtcgcgcg gagctcctcg
    29941 cgctcgagcg tcccgacggg cccgggtccc tccgccgcga gatcgtcgag cgggcggtcc
    30001 tccgtggaca ccacggctcc cgccgcgggc ctcttcgcaa gccgccgctg cgcctcgttg
    30061 aagccgatcc ggtagagcca cgtgaagaac cgcgagcgcg cctcgaagcg cgacagcgat
    30121 cgccatgccc gcaggaaggt ctcctgcgcg acgtcctgcg cctcgccgtg gtcgaggccg
    30181 aaccgcagca gcgctccgtg gagcgcatcg gcgtgcctca tcaccagctg ctcgaaggcg
    30241 gccatgtcgc cggcgctcgc ctgctggacc agcaccgcgt cgggatcctg cgccgtcccg
    30301 acctcaggcg cgcgagtcat cgtctgccag tcggtcgtgt acccccgcca gcaccgccgc
    30361 cgccacggat tggcccggcc gtcccggcag cgctgccagg gtcgcgacca tcatccggag
    30421 gctggcgagc agctccgcgc actccgggca gtcgtccgtg tggcgctcga tccggccggc
    30481 cccgcgggca ccgagctccg cgtcgatgta ctccgacatg tgctgctgcg accatgcgtg
    30541 gtcgcgcgcg aagctaagcg ccgtaaacgg attcctcatc ggcaattaga ccctacgtgc
    30601 ggcgacccga ttcggatgac ctgctgaacc gttttacgag acgccgggtc gtacccccca
    30661 tgacgacgac gagaatcgag gcattcgcat gacgagcgcc tcagccgtcg gcccgctcgc
    30721 gcgcctcgcc gacgtgcgca cccggacgct cgagctcgtc gagccgctct ccgattccga
    30781 tgccgaggcc gtccactcgc cgctcatgag cccgctcgcg tgggacctcg gccacatcgc
    30841 ggcgttcgag gatctctggc tggtgcaccg cgcggcgggc gagccgctgc tccgacccgg
    30901 cctggccgag atctacgacg ccctcgagac cccgcgagcc caccggggcg agctccagtt
    30961 cctcggccgc tcggcggcgc tcgattacct ggccgaggtc cgcggccggg tcgaggagct
    31021 ggcgccgagc gacgtgctgc tccaggagct cgtgatccag cacgagctcc agcacaacga
    31081 gacgatgctc cagacgctcg cgctcgcgcg actcgacggc tacgtgccgc ccggctacgt
    31141 gtccccggcc gaccagcggc tgccccaggt cgagcagcgc acgcccaccg ggctcgaaac
    31201 ggtggtcgtg ccgcccgggg cgttcgagct cggagcgccc gcgggccgct tctcgtacga
    31261 caacgagcgg ccacggcaca ccacggacgt cccggcgttc gagatcgggc gcgtgcctgt
    31321 gacgaacggc gccttcggag agtggatcgc cgcgggcgga tacgcacgcc gtgagtggtg
    31381 gtcggaggcc ggctgggagt ggaggcgggc gtccggcgtg gagcggccgg gctcctggtt
    31441 cgac
//
LOCUS       DASVPL010000029         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_159524, whole genome shotgun sequence.
ACCESSION   DASVPL010000029 DASVPL010000000
VERSION     DASVPL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159524"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /gene="obgE"
                     /locus_tag="VF517_01610"
     CDS             <1..1304
                     /gene="obgE"
                     /locus_tag="VF517_01610"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX8051660.1"
                     /translation="DRAKVRVQAGAGGDGVVSFRREAHVPRGGPDGGDGGRGADVVLV
                     CDPSLRDLEEFRYRKLYQGARGGHGQGSNRHGASPDALVVGVPPGTRVEDGERGLSWE
                     LAVAGERVVVARGGLGGRGNRHFTTATRQAPRFAERGLPGEEGWLELRLRLLADAGLV
                     GLPNAGKSSLLARITRAAPKVANYPFTTLAPVLGTLESDDRQLVVADIPGLIEGASEG
                     AGLGHEFLAHVEKTRLLVHVLDIAPVDGSDPVASHATVEAELAAHGAGLERLPRILAL
                     SKSDLVPEERAEEARREWRERLGERVREVLVTSAATGRGLDDLRRAILEGVPAEPVPE
                     APAAAPDLDGLAEHRTYRPAGDGFQVERAGEGAFRVAGPRVERLLARHDPDNDDAMRY
                     VEERLRGMGVIAALEAAGFEPGDDVEIGGVVFELDPGAPFG"
     gene            1315..>2327
                     /gene="proB"
                     /locus_tag="VF517_01615"
     CDS             1315..>2327
                     /gene="proB"
                     /locus_tag="VF517_01615"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX8051661.1"
                     /translation="MKERVVVKVGSNTVLDRAGRFRPHLAVLATELAALHRGGADVAL
                     VTSGGIARGLRSLHVTRRTKRMKRDLQAASAVGQPDVYSHFEQLFARQRPRLATAQVL
                     FTFAEITELHHFRNVQQALTQLVEWRVVPIINENDTTTMDDITFENNDYLAAQVANML
                     RATRLVLFTNVDGVFTANPQASSGAELIEEITDVKDALTRYRIKKGAAQLGTGGMYSK
                     VVAAEMAAASGTRVTIANGVKADSLRAALRGERVGTIFPPAAGRQKKVFPRRDWIRSR
                     RPKGSIYVDAGAARALRTAGASLLPVGVVRVGGSFGREAIVDVLHEDELLGKGIVALS
                     ASQL"
BASE COUNT          312 a          804 c          914 g          297 t
ORIGIN      
        1 acgaccgcgc caaggtccgc gtgcaggcgg gggccggggg agacggcgtc gtctcgttcc
       61 gccgtgaggc gcacgtgccg cgcgggggtc ctgacggcgg cgacggcggc cgcggggccg
      121 acgtggtcct ggtctgcgac ccgtcgttgc gcgacctcga ggagttccgc taccgcaagc
      181 tctaccaggg cgcgcgcggg gggcacggcc aggggtcgaa caggcacggg gcctcgcccg
      241 acgctcttgt ggtgggcgtg ccgcccggca cgcgggtgga ggacggcgag cgcggcctga
      301 gctgggagct cgcggtagcg ggcgagcgcg tggtggttgc gcgcggcggg ctcggcgggc
      361 gcggcaaccg ccacttcacg accgccacgc ggcaggcgcc gcggttcgcc gagcgcgggc
      421 tccctggcga ggagggctgg ctcgagctgc ggctgcggct gctggccgac gccgggctcg
      481 tggggttgcc gaacgcgggc aagtcgtcgc tgctcgcgcg catcacccgc gctgcgccga
      541 aggtggcgaa ctatccgttc acgacgctcg cgccggtgct cggcacgctc gagagcgacg
      601 accgccagct cgtggtcgcc gacatccccg gcctgatcga gggggcgagc gagggcgccg
      661 gcctcggaca cgagttcctc gcgcacgtgg agaagacgcg gctgctggtg cacgtgctcg
      721 acatcgcgcc ggtggacggg tcggacccgg tggcgagcca cgcgaccgtc gaggccgagc
      781 tggcagcgca cggcgcgggc ctcgagcgcc tgccgcgcat cctcgcgctg tcgaagtcgg
      841 acctcgtgcc cgaggagcgc gccgaggagg cgcggcggga gtggcgcgag cgcctcggcg
      901 agcgcgttcg cgaggtgctc gtgacctccg cggccaccgg gcgcggcctc gacgacctca
      961 ggcgcgcgat cctcgagggc gtgccggcgg agccggtgcc cgaggcgccc gccgccgcgc
     1021 cggacctcga cggactcgcc gagcaccgca cgtaccgccc cgccggcgac ggcttccagg
     1081 tcgagcgcgc gggggagggg gctttccgcg tcgcgggccc gcgcgtggag cggctgctcg
     1141 cccgccacga ccccgacaac gacgacgcga tgcgctacgt cgaggagcgg ctgcgcggca
     1201 tgggggtgat cgcggcgctc gaggcggcgg ggttcgagcc gggcgacgac gtggagatcg
     1261 gcggggtcgt gttcgagctc gatcccggcg cgccgttcgg gtagcgtcgc caccgtgaag
     1321 gagcgcgtag tcgtgaaggt ggggtcgaac acggtgctcg accgagcggg gcggttccgc
     1381 ccgcacctcg ccgtgctcgc caccgagctc gccgcccttc accggggcgg cgcggacgtc
     1441 gccctcgtga ccagcggcgg catcgcacgc ggcttgcggt cgctccacgt cacccgcagg
     1501 acgaagcgca tgaagcgcga cctccaggcg gcgagcgccg tgggccagcc ggacgtgtac
     1561 tcgcacttcg agcagctctt cgcccgccag cgcccgcgcc tcgcgacggc ccaggtgctg
     1621 ttcacgttcg ccgagatcac cgagctgcac cacttccgca acgtccagca ggcgctcacg
     1681 cagctcgtcg agtggcgggt ggtcccgatc atcaacgaga acgacacgac cacgatggac
     1741 gacatcactt tcgagaacaa cgattacctc gctgcgcagg tggcgaacat gctgcgagcc
     1801 acgcgactgg tgttgttcac gaacgtcgac ggcgtcttca ccgcgaaccc tcaggcgagc
     1861 agcggggcgg agctgatcga ggagatcacc gacgtcaagg acgccctcac gcggtaccgg
     1921 atcaagaagg gggccgccca gctcggcacc gggggcatgt actccaaggt ggtcgccgcg
     1981 gagatggccg cggcgtcggg tacgcgggtg acgatcgcga atggcgtgaa ggcggattcc
     2041 ctgcgcgcgg cgctccgggg ggagcgtgtc gggacgatct tccctccggc cgctggccgg
     2101 cagaagaagg tcttcccgcg ccgcgactgg atccgcagcc gccgtcccaa gggttcgatc
     2161 tacgtggacg cgggcgccgc acgggcgctg cgcacggcgg gagccagtct gctcccggtg
     2221 ggcgtcgtac gcgtcggggg cagcttcggg cgcgaggcca tcgtcgatgt gctgcacgag
     2281 gacgagctgc tcgggaaggg gatcgtcgcg ctgtcggcga gccagct
//
LOCUS       DASVPL010000030         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_81805, whole genome shotgun sequence.
ACCESSION   DASVPL010000030 DASVPL010000000
VERSION     DASVPL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81805"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VF517_01620"
     CDS             <1..639
                     /locus_tag="VF517_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051662.1"
                     /translation="GVQFTRLAVGDGGQGAALVQQDGRMYLVTDVAGNGTRDCVPDYY
                     PEYNPSYYDDDPYLPRTHGGPGGGAWEFDDDPYSGPGGSGAPPANPVPVGSPAISAAR
                     AGQRKRTARVVVVCRTACTIDARARIAFAGQAKALVTGTARTSSNGKPKLVEIPLTMS
                     ASTAKTIDAALRDKKPKRALLLTIGVTATIPKQKKQTREVTILLGPSASPFR"
     gene            complement(655..>1971)
                     /locus_tag="VF517_01625"
     CDS             complement(655..>1971)
                     /locus_tag="VF517_01625"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX8051663.1"
                     /translation="AGDDPAQSGQTAVPRSDRPLPVRMRPTTADELVGQQHLLGPESP
                     LRRALDDGEPHSMILYGPPGTGKTTIARMLAVNANAAFEELSAVNAGRAEVRTVLARA
                     EERVRASGRHTIFFLDEIHRFNKAQQDALLPAVEEGLVSLVGATTENPYFEVNSALIS
                     RTRIYELQLLSDDDITRLLRRALDGFIDDPPEIDDEALAFLAARSAGDARSALVGLEL
                     AAENPDDGRITMAVAEQAMQRKAVLYDKGGDHHYDTISAWIKATRGSDPDASLLYLAA
                     MIEGGEDPRFIARRMVILASEDVGNADPRALGVAVDTAYAVEHVGMPECAHNLAQCSV
                     YLALAPKSNASYKALKAAREWVRENGPVIPPDSMRSAAYPGAKALGRGKGYDYPHDRP
                     EGVSPQELMPQEAEGERFLDLSEHGEERDLRERQAKIERARGRRDS"
BASE COUNT          257 a          730 c          713 g          271 t
ORIGIN      
        1 ggcgtgcagt tcacccgcct cgccgtcggc gacggcggcc agggcgccgc gctggtgcag
       61 caggacgggc gcatgtacct cgtgaccgac gtcgccggca acggcacgcg cgactgcgtg
      121 cccgactact accccgagta caacccgtcg tactacgacg acgacccgta cctgccgcgc
      181 acgcacggcg gtcccggcgg cggcgcgtgg gagttcgacg acgaccccta ctccgggccg
      241 ggcgggagcg gcgcgccgcc ggcgaacccg gtgccggtcg ggagcccggc gatcagcgcc
      301 gcgcgcgccg gtcagcgcaa gcgcaccgcg cgcgtggtcg tggtgtgccg cacggcgtgc
      361 acgatcgacg cgcgggcgcg gatcgcgttc gcgggccagg ccaaggcgct cgtgacgggg
      421 acggcccgca cgagctcgaa cggcaagccg aagctggtgg agatcccgct gacgatgtcc
      481 gcctcgaccg cgaagacgat cgacgcggcc ctcagggaca agaagccgaa gcgcgcgctg
      541 ctgctcacga tcggcgtgac cgcgacgatc cccaagcaga agaagcagac gcgcgaggta
      601 acgatcctcc tcgggccctc ggccagcccg ttccgctgag ggcgggctga cccgctagga
      661 gtcgcggcgc ccgcgggcgc gctcgatctt cgcctggcgc tcgcgcaggt cgcgctcctc
      721 gccgtgctcg gagaggtcga ggaagcgctc gccctcggcc tcctgcggca tcagctcctg
      781 cggcgagacc ccctcgggtc gatcgtgcgg gtagtcgtag cccttgccgc gcccgagcgc
      841 cttcgccccc gggtacgcgg cgctgcgcat cgagtccggc gggatcacgg gaccgttctc
      901 acgcacccac tcccgcgcgg ccttgagagc cttgtaggac gcgttcgact tgggtgccag
      961 cgctaggtag acggagcact gcgcgaggtt gtgcgcgcac tccggcatcc ccacgtgctc
     1021 gacggcgtac gcggtgtcga ccgcgacgcc gagcgcgcgc gggtccgcgt tgcccacgtc
     1081 ctcgctcgcg aggatcacca tccgccgggc gatgaagcgc gggtcctcgc cgccctcgat
     1141 catcgccgcg aggtagagca gcgacgcgtc ggggtccgat ccgcgcgtgg ccttgatcca
     1201 ggccgagatc gtgtcgtagt ggtggtcgcc gcccttgtcg tagagcacgg ccttgcgctg
     1261 catcgcctgc tcggcgaccg ccatcgtgat ccgcccgtcg tccgggttct ccgcggccag
     1321 ctcgagcccc acgagggccg agcgcgcgtc gccggccgag cgggccgcga ggaacgccag
     1381 cgcctcgtcg tcgatctccg gcgggtcgtc gatgaagccg tccagcgccc gccgcagcag
     1441 cctggtgatg tcgtcgtccg acaggagctg gagctcgtag atccgggtgc gcgagatcag
     1501 cgcgctgttc acctcgaagt acgggttctc ggtggtcgcg ccgacgagcg acacgaggcc
     1561 ctcctccacc gccggcagca gcgcgtcctg ctgcgccttg ttgaaccgat ggatctcgtc
     1621 gaggaagaag atcgtgtggc ggccgctcgc gcgcacgcgc tcctccgccc gcgccagcac
     1681 cgtgcgcacc tcggcgcgcc ccgcgttcac cgccgagagc tcctcgaagg ccgcgttcgc
     1741 gttcaccgcg agcatgcgcg cgatcgtggt cttgccggtc cccggcggcc cgtagaggat
     1801 catcgagtgc ggctcgccgt cgtcgagcgc gcgccgcagc ggggactcgg ggccgagcag
     1861 gtgctgctga cccacgagct cgtccgccgt ggtgggccgc atgcgcaccg gcagcgggcg
     1921 gtccgaccgc gggaccgccg tctgcccgga ctgcgcgggg tcgtcgccgg c
//
LOCUS       DASVPL010000031        14213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_316249, whole genome shotgun sequence.
ACCESSION   DASVPL010000031 DASVPL010000000
VERSION     DASVPL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14213
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316249"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VF517_01630"
     CDS             <1..514
                     /locus_tag="VF517_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX8051664.1"
                     /translation="VAAGLRPGAGAGEVAEACVLGAALGVRHGRAVALPPVNERIELA
                     LDAARAARDLDDALGRIESAVGTSMLAHESVPAAVGVFAAAGGNPLEAVRAGVLIGGD
                     TDTIAAMAGALAGALRGYACLKSGDRALLDAVTPPALAQLVPGLAAIAARRARAQPGA
                     SARSRPAAAD"
     gene            complement(511..1845)
                     /locus_tag="VF517_01635"
     CDS             complement(511..1845)
                     /locus_tag="VF517_01635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8051665.1"
                     /translation="MAEYDVIIVGQGAPGEHCAARLVEGGAKVAVVERELIGGECSFW
                     ACIPSKTLLRPGEALQAAREAPGAITGELDVERVLGWRDFQVSSYDDSGGADWLESVG
                     VELVRGEGRIAGPGRVAVGERELTADHVIVATGSEPIIPPVPGLRELDGVWTNREATG
                     LKGVPRRLIVLGGGPVGVEMAQAVRRMGAEVALVEGSEHVLAREPKRLGEALGERLAE
                     EGIELRFGVHVSAARRDGDDYVLELEDGSELRGDRLLVATGRRPRSGGIGLENAGVEL
                     GEHGEVPVDSRMSAGDGIWAIGDVTGIWPLTYVGKYQGRVAAANILGTPREANYDAVP
                     RVVFTDPQAASVGAAEGEREVTIDLSGVARTSTYTREYEHAGFMTLVSDGEVVTGAYA
                     LGPEAGEWLQQATLAIRAKVPLAVMADTIQPFPTFSEAFMEAFLELGSEPAG"
     gene            1875..3239
                     /locus_tag="VF517_01640"
     CDS             1875..3239
                     /locus_tag="VF517_01640"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX8051666.1"
                     /translation="MSDATLKFLLPESEIPTHWVNLMPDLPGPPPSPPLHPGTKEPAG
                     PDDLSPIFPMGLIEQEVSTEPEVEIPDEVRDVYRRWRPSPLYRARAFERELGTSAHIY
                     FKYEGVSPAGSHKPNSAVPQAYENKQAGIEKLVTETGAGQWGSALALASALFGIECEV
                     YMVGSSYDQKPYRRALMETWGATVHRSPSDRTESGRANASHETGSLGIAISEAVEVAA
                     GSDANYALGSVLNHVCLHQTVIGQEAIAQMGMAGEEPDVVIGCVGGGSNFAGIAIPFV
                     RRVLRDGAKTRFVAAEPAACPTLTRGAYRYDFGDTSGMTPLLPMYTLGHDFVPPPLHA
                     GGLRYHGDSPLVCGLVKAGLVEAVAFRQNECFDAALRFARTEGIVPAPEAAHAVRAAV
                     VEAAAADEAGEQRAILFNLSGHGHFDMSAYQAYLSGSLEDVEFSEADMEAALERLPEA
                     PSLA"
     gene            3332..3793
                     /locus_tag="VF517_01645"
     CDS             3332..3793
                     /locus_tag="VF517_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX8051667.1"
                     /translation="MHGRAAKRTVRHYDLDYDYQSRDVVPADPLPAEVEWLRERCAEL
                     AEKRPDELAQVLVSRYPPGAGIGWHRDAPMFGRVVGVSLGAPARMRFQRTTKEGERLV
                     EELDLEPRSGYLLSGSARWTWQHSIPAQKGLRHSITFRTLREPEPRARSAP"
     gene            complement(3747..4211)
                     /locus_tag="VF517_01650"
     CDS             complement(3747..4211)
                     /locus_tag="VF517_01650"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEX8051668.1"
                     /translation="MHQIAQLNLARAKAPLDSPELAEFMALLEPINALADGSPGFVWR
                     LQDDDGAGATGIRIADEDRVIVNISVWETIDALWAYVYESAHLEVMRRRREWFVHFGK
                     PFLVLWWVPAGHEPTVAEALERLALLESEGPSPDAFTFKERFAPAAPAPAAS"
     gene            complement(4211..4975)
                     /locus_tag="VF517_01655"
     CDS             complement(4211..4975)
                     /locus_tag="VF517_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051669.1"
                     /translation="MENLGPSIGWGLAIAASLLAGAVAAAALRLPERVAALLTAFGGG
                     TLLAAIALELVPEADAGAGPALTAAGLVAGTAVYVGADYLLTRNSGMRAHRRAAHAVA
                     AGRPMNMPDHGESARGEAIAAGIFIDGVPESIAIGLTVAEGEIGVALLAGVLIGNVVE
                     AYGAAQPIVKGGHTSGFAIRLLGGIGLTLAAATVLGATVLADASEELVGGAQAVAAGA
                     VLAVVSIAIIPHAFSEVSRLTAAATVAGFVAGYLLS"
     gene            5062..5853
                     /locus_tag="VF517_01660"
     CDS             5062..5853
                     /locus_tag="VF517_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8051670.1"
                     /translation="MIVDGISVRHADRGAARVEGDLPVLVLHGWGAHLEAIEPIVAAL
                     AGETRVLALDLPGFGQSDPPPEPWDADGYMRFVLRFLDELGVERVHVVGHSHGGRVAI
                     CMAAEHPERVARLLLVDAAGVPPKRGMKYRAKVSVAKAGKVAGRVGASGLQDRLRARV
                     GSTDYLNASEEMRATFRLVIGQDMTERLPRIGATTLLVWGDQDDDTPLWMAHRMEELI
                     PDAGLAVFEGAGHYSYADDPARFGSVARLFLCEQPRALAAGAPAA"
     gene            5850..7490
                     /gene="murF"
                     /locus_tag="VF517_01665"
     CDS             5850..7490
                     /gene="murF"
                     /locus_tag="VF517_01665"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX8051671.1"
                     /translation="MIATGFDLAFAGLFGLMAGRLHVLLHLFQLEHYEPARLRVWVKR
                     RGLRLDPNELGYLTGFAGLMVAATGFDLPGVVLGLGALAAIVGVRHGASVLRRDQIKP
                     LVFTPRARRMYATALGLLLLAAIAISVGAIVGDRESLAANLGLLLALGSYVLAPELLI
                     LANLLVKPVQALDNRRFVARARRKLAEVDPLVVGITGSYGKTTTKSCVAAVAELRGPA
                     YPTPASFNSFLGVVRAINEGLQSRHRTFVVEMGAYRRGDVAELCELAGPKIGILTAIG
                     PAHLERFGSLDETERAKGELGESLPADGALITRADDERCRRAAQRSPAPVRLFAPFPH
                     VDADVWAEDVELRDGRTVFRLCARDADAVTVKARLLGEHNVANLVAAAAAGLEMGLGL
                     DAIGRALAKVQPPAHRLAPIVNASAGVVVIDDAYNSNPQGAAAALDVLASHPADRRLL
                     VTPGMVELGDRQEEENRRFGERAAAVCDLVVLVGPEQTRPVRAGLEAAGFADSALHVV
                     PDAGAAQELLARTTRRGDVVLFENDLPDLYASGNGSGG"
     gene            7505..8734
                     /locus_tag="VF517_01670"
     CDS             7505..8734
                     /locus_tag="VF517_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051672.1"
                     /translation="MRVAVCFGGPSVEHDVSIISAQQLMAALEDRHEPVPIYLARDGR
                     WWTGDSLREIGSYGSDRPTGAEPVELRLGSSGAPFAVPAASRFRGGRDVPVDVAINAV
                     HGTGGEDGALLGALEQTDVPYAGGGVGPAAAAMDKALAKLVFRDAGVDVNPHLQVTRE
                     RFAGDPAGAAAEAVEAVGLPCFAKPSTLGSSIGVTRCADAAELEEALELCFELDRRAL
                     VEPSLEEAIEINAAVLGRPGGELRVSVLEQPVKGDAALSFEDKYLRAGGKGGAKDGAP
                     GSAPSGPKAGSSAGAKAGGGDGAGMASADRIIPAPIDEELADRIRQAALAAHRALGFA
                     GVVRYDFFVHEDRVILNEANTVPGSFAFYLYEPDGLTFPDLADALVEIALAEAAERRA
                     TTRTFESVLLSRHQGGG"
     gene            8734..9684
                     /locus_tag="VF517_01675"
     CDS             8734..9684
                     /locus_tag="VF517_01675"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX8051673.1"
                     /translation="MPGRVVVASGGRSLEREISLRSGRRATDALEALGYDAIPIDPDH
                     GFVGEIKRLRPEFVFVAMHGRGGEDGTLQDLLEILHVPYTGSDVHASARCLDKHAFKE
                     LMVGIGLATPVWHSFNRDAFAAFGAADTLPELIAQLGFPLVVKPAREGSSLGIKFVNS
                     SEEFSQAVVGALSYDDRVVIEHFVEGRELAATVLGPPDAPEVLPVVEIKTDEPFYTFR
                     AHYDAGVAELEVASLEPGVQESVESAARKAYTAAGCRDFARVDIILDTENEPQILEIN
                     TIPGLTETGPARFAAEAAGLDFPALVERVVARAAGEAGGA"
     gene            complement(9866..10447)
                     /locus_tag="VF517_01680"
     CDS             complement(9866..10447)
                     /locus_tag="VF517_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX8051674.1"
                     /translation="MGRLATAAAVVLTLALAAPAAAQEEQPTGGAAPPGQPSGAQAPV
                     EGMPAVPFANEIATAADANGVDRLLLAALVHAESNFDPEATSRSGARGLAQLMPSTAR
                     SLGLRVDLKRGVDDRIVPARSLDAGARYLRSQLDRFRGKIQLSLAAYNAGPRAVIRYR
                     GIPPYRETRAYVRKVVGYRNEYRAELAAARDSG"
     regulatory      complement(10473..10612)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            10732..11625
                     /locus_tag="VF517_01685"
     CDS             10732..11625
                     /locus_tag="VF517_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051675.1"
                     /translation="MPHLFRSRPVGLQVVLAGIVPALFGALTGWVLGLSEIGYLVLSL
                     LALAGAYAAGLEHDGAGEAALRGALGGALFGGFILIVHNATGEEAKAELPDPEILLIA
                     VTIAFSVGAGALGGRRRHRHEAEGPKDKGEKEAVNFKRLHWAEFIGFAGVGVLLASMF
                     LPWFSTSCDSLRPATPDGCNPNSTLNGLRGDFNAFETYKIMDILLVLACIAPFVLAYL
                     VIRESQLSWRPGEVTMIVGMVAAALIWLNGIILGRPGDSVDIGIEIGYWIGLIGANMI
                     LAGGLIRQALGGRTRKPPGTL"
     gene            11628..12626
                     /gene="glpX"
                     /locus_tag="VF517_01690"
     CDS             11628..12626
                     /gene="glpX"
                     /locus_tag="VF517_01690"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX8051676.1"
                     /translation="MPDPTGARPDRNLAMELVRVTEYGALAAARWVGLGEKEKADGAA
                     VDAMRLMLDTVRMDGVVVIGEGEKDEAPMLYNGEQIGDGSPPQVDIAVDPLEGTTLAA
                     EGSPRALAVMAMSERGSMFNPGPAVYMLKIAAQPAFAHLLDLDRPLSETLELIAREKG
                     TDVRDVTVMMLKRDRHNEAVEEIRRAGARIRFISHGDVSASLLAVQDESPIDLLWGIG
                     GTPEGVISAAAIKCLGGQMLGRVWPRSDEERQAIADFGLDLDEVLDVDRLIAGDDVFF
                     SATGVTDGDVLEGVHYTTTGATTESLVMRTRSGTVRRVSAVHDRAKLREISGQRAG"
     gene            complement(12630..13367)
                     /locus_tag="VF517_01695"
     CDS             complement(12630..13367)
                     /locus_tag="VF517_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051677.1"
                     /translation="MRPRDPRRLLERRRRAVARVNIVATPQGSSTEDGSVTTKQVADV
                     TLPRAELDKLWRAEYLERLARTYWVFLSRFTLGLIRVLYTPEGREVVLVARPLRLLTF
                     RPPEYETLPHRGTVTWRIEKGILVAPQGRNKGHLRVCVERPQPDPGGDEINARVSSEV
                     ANFYPAIAQSWVPRKLARLGAWLYQQTQLRIHVIVTNAFLRSLARLELPESVVGSLRE
                     EARKALAGENPAADPVAEQRAEQVQSA"
     gene            complement(13364..>14213)
                     /locus_tag="VF517_01700"
     CDS             complement(13364..>14213)
                     /locus_tag="VF517_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEX8051678.1"
                     /translation="TLAPGANIRRAGEDVRAGEVVLRRGAQIGPSELAMLAALGRTEV
                     RCGAVPRVAVVVAGDELVDPGAPLGPGQIRDSNGPALVALATASGARVDGHRRVGDSH
                     AATVAALRAALDKADVVCGSGGVSVGEHDHVKPALAELGVEQIFWGVALKPGKPTWFG
                     ATPTGALVFGLPGNPVSAMVTFQLFAHPAIRALAGADPAPARVRARLTAPVTRSERRE
                     QAIRCRLTASDDGWSAEPTGAQGSHRISSMLGAGALALIPRGTGELAPGALVDAEPFP
                     LGTFQG"
BASE COUNT         1835 a         5198 c         5279 g         1901 t
ORIGIN      
        1 ggtggccgcc ggcctgcggc ccggtgccgg agcgggcgag gtggccgagg cgtgcgtgct
       61 gggagccgcg ctcggcgtgc ggcacggccg ggcagtcgcg ctgccgcccg tgaacgagcg
      121 gatcgagctg gcgctggacg cggcgcgggc ggcgcgcgac ctcgacgacg cgctggggcg
      181 gatcgagtcc gcagtgggca cgtcgatgct cgcgcacgag tccgtaccgg cggccgtggg
      241 cgtgttcgcc gcggcgggcg gcaatccgtt ggaggccgtg cgggccggcg tgttgatcgg
      301 cggcgacacc gacacgatcg ccgcgatggc cggcgcgctc gccggcgcgc tgcgcgggta
      361 cgcgtgcttg aagtccggcg atcgcgcgct tctggatgcc gtgacgccgc ccgcgctggc
      421 ccagctcgta cccggcctgg cggcgatcgc cgctcggcgc gctcgcgccc agcccggcgc
      481 ctcggcccgc tcgcgcccgg cggccgccga ctagcccgcg ggctccgacc ccagctccag
      541 gaacgcctcc atgaacgcct ccgagaacgt ggggaagggc tggatcgtgt ccgccatcac
      601 cgcgagcggc accttcgcgc ggatcgcgag cgtcgcctgc tggagccact cgcccgcctc
      661 gggcccgagc gcgtaggcgc cggtcacgac ctcgccgtct gagacgagcg tcatgaaccc
      721 ggcgtgctcg tactcgcggg tgtaggtgga cgtgcgcgcg acgccgctca ggtcgatggt
      781 cacctcgcgc tcgccctcgg ccgcgccgac cgacgccgcc tgcgggtccg tgaacaccac
      841 gcgcggcacc gcgtcgtagt tggcctcgcg cggcgtgccg aggatgttcg cggcggccac
      901 gcggccctgg tacttgccga cgtaggtgag cggccagatg ccggtcacgt cgccgatcgc
      961 ccagatcccg tcgcccgcgg acatgcgcga gtccaccggc acctcgccat gctcgcccag
     1021 ctccactccg gcgttctcga ggccgatccc gccggaccgc ggccgccggc ccgtcgccac
     1081 gagcagccgg tcgccgcgca gctcgctccc gtcctcgagc tcgagcacgt aatcgtcgcc
     1141 gtcgcggcgg gccgcgctca cgtgcacgcc gaacctgagc tcgattccct cctccgcgag
     1201 ccgctcgccc agcgcctcgc cgagccgctt cggctcgcgc gcgagcacgt gctcggagcc
     1261 ctccacgagc gccacctccg cgcccatgcg gcgcacggcc tgcgccatct cgacgccgac
     1321 cggcccgccg ccgagcacga tcaggcgccg cgggaccccc ttcaggcccg tggcctcgcg
     1381 gttggtccac acgccgtcga gctcgcgcag cccgggcacc ggcgggatga tcggctcgga
     1441 gcccgtcgcg acgatgacgt ggtcggcggt cagctcgcgc tcgcccaccg cgacccgccc
     1501 cggcccggcg atccggccct cgccgcgcac gagctcgacg ccgaccgact cgagccaatc
     1561 cgcgccgccg gagtcgtcgt agctcgacac ctggaagtcg cgccagccga gcacgcgctc
     1621 cacgtccagc tcgcccgtga tcgcgcccgg cgcctcgcgc gcggcctgga gcgcctcgcc
     1681 cgggcgcagc agcgtcttcg acgggatgca cgcccagaac gagcactcgc ccccgatcag
     1741 ctcgcgctcc accacggcga ccttcgcgcc gccctccacc agtcgcgccg cgcagtgctc
     1801 gcccggcgcg ccctggccca cgatgatcac gtcgtattcg gccatgcgcc cagtatcccg
     1861 gtaccgtccg gcgcatgagc gacgccaccc tgaagttcct cctgcccgag tccgagatcc
     1921 cgacgcactg ggtcaacctc atgcccgacc tgcccgggcc gccgccgtcg ccgccgctgc
     1981 acccgggcac gaaggagccc gccgggccgg acgacctctc gccgatcttc ccgatggggc
     2041 tgatcgagca ggaggtctcg accgagccgg aggtcgagat ccccgacgag gtccgcgacg
     2101 tctaccggcg ctggcgcccg tcgccgctct accgcgcccg cgcgttcgag cgcgagctgg
     2161 gcacctcggc gcacatctac ttcaagtacg agggcgtgtc gccggccggc tcgcacaagc
     2221 cgaacagcgc cgtgccgcag gcgtacgaga acaagcaggc ggggatcgag aagctcgtca
     2281 ccgagaccgg cgcggggcag tggggctcgg cgctcgcgct cgcgtccgcg ctgttcggga
     2341 tcgagtgcga ggtctacatg gtcgggtcga gctacgacca gaagccgtac cggcgcgcgc
     2401 tgatggagac ctggggcgcg accgtccacc gctcacccag tgaccgcacc gagtccggcc
     2461 gggcgaacgc ctcgcacgag accggctcgc ttgggatcgc gatctcggag gccgtcgagg
     2521 tggcggcggg gtcggacgcg aactacgcgc tcgggtcggt gctcaaccac gtgtgcctgc
     2581 accagaccgt gatcgggcag gaggcgatcg cgcagatggg gatggcgggg gaggagcccg
     2641 acgtggtgat cggttgcgtc ggcggcggct cgaacttcgc cgggatcgcg atcccgttcg
     2701 tgcggcgcgt gctgcgcgac ggggcgaaga cgcgcttcgt cgccgccgag ccggcggcct
     2761 gcccgacgct cacgcgcggg gcctaccgct acgacttcgg cgacacctcc gggatgacgc
     2821 cgctgctccc gatgtacacg ctcggccacg acttcgtgcc gccgccgctg cacgccggcg
     2881 gcctgcgcta ccacggggac tccccgctcg tctgcgggct ggtcaaggcg gggctcgtcg
     2941 aggcggtggc cttccggcag aacgagtgct tcgacgcggc gctgcgcttc gcgcggaccg
     3001 agggcatcgt gcccgcgccc gaggcggcgc acgcggtgcg cgcggccgtg gtcgaggccg
     3061 cggccgccga cgaggcgggc gagcagcgcg cgatcctctt caacctctcc ggccacggcc
     3121 acttcgacat gtccgcgtac caggcgtacc tgtccggctc gctcgaggac gtggagttct
     3181 cggaggccga catggaggcc gcgctcgagc ggctgcccga ggcgccgtcg ctggcctgaa
     3241 ggggctcgtc tacaggcccg acctggtcac ggtcgcggag gaggagcagg tcctcgcgtg
     3301 gctcgacacg atcgactacc gcgaggtggt gatgcacggc cgcgcggcga agcgcaccgt
     3361 gcgccactac gacctcgact acgactacca gtcgcgcgac gtcgtgccgg ccgaccctct
     3421 tcccgccgag gtcgagtggc tgcgcgagcg ctgcgccgag ctggccgaga agcgcccgga
     3481 cgagctggcg caggtgctgg tctcgcgcta cccgccgggc gccggcatcg gctggcaccg
     3541 cgacgccccg atgttcggcc gcgtggtggg cgtatcgctc ggagcgcccg cgcggatgcg
     3601 cttccagcgc acgacgaagg agggcgagcg gctggtcgag gagctggacc tcgagccgcg
     3661 ctcgggctac ctgctgtcgg gcagcgcgcg ctggacgtgg cagcactcga tcccggccca
     3721 gaaggggctg cgccactcga tcacgttcag gacgctgcgg gagccggagc cgcgggcgcg
     3781 aagcgctcct tgaacgtgaa cgcgtccggc gacgggcctt ccgactcgag cagcgccagc
     3841 cgctccagcg cctccgccac ggtcggctcg tgccccgccg ggacccacca gaggaccagg
     3901 aacggcttgc cgaagtgcac gaaccactcg cgcctgcgcc gcatcacctc gaggtgcgca
     3961 ctctcgtaga cgtacgccca gagcgcgtcg atcgtctccc agacggagat gttcacgatc
     4021 acccggtcct cgtcggcgat gcggatgccg gtcgcgccgg ccccgtcgtc gtcctgaagg
     4081 cgccacacga acccaggact gccgtccgcc agggcgttga tcggctcgag cagcgccatg
     4141 aactcggcca gctcgggcga gtcgagcggc gccttcgcgc gcgcgaggtt gagctgcgcg
     4201 atctgatgca tcagctcagc aggtagcccg ccacgaagcc ggcgaccgtc gcggccgccg
     4261 tgaggcggct gacctcggag aacgcgtgcg ggatgatcgc gatcgatacg accgcgagca
     4321 cggcgccggc cgccacggcc tgggcgccgc cgaccagctc ttcgctggcg tcggcgagga
     4381 ctgtcgcgcc gagcacggtg gccgcggcga gcgtgagccc gatgccgccg agcaggcgga
     4441 tcgcgaaccc gctcgtgtga ccgcctttca cgatcggctg cgccgccccg taggcctcca
     4501 ccacgttgcc gatcagcacg cccgcgagca gcgcgacgcc gatctcgccc tccgcgaccg
     4561 tcagcccgat cgcgatcgac tcgggcacgc cgtcgatgaa tatcccggcg gcgatggcct
     4621 cgccgcgggc ggactcgccg tggtcgggca tgttcatcgg gcgtccggcc gctaccgcgt
     4681 gggcggcgcg gcggtgcgcc ctcatgccgc tgttgcgagt cagcaggtag tcggcgccga
     4741 cgtagaccgc cgtgcccgcc acgagcccgg ccgccgtgag ggcggggccg gcgccggcgt
     4801 ctgcctcggg caccagctcg agggcgatcg cggccaggag ggtgccgccg ccgaacgcgg
     4861 tgagcagcgc ggccacgcgc tccgggaggc ggagcgcggc ggccgccacg gcgcccgcga
     4921 gcaggctcgc cgcgatcgcg aggccccagc cgatcgacgg acccaggttc tccacgcccc
     4981 gattgtcgcg cacctcgggg agaggaaggt cgcgcacctc gcatggagga agccgccagt
     5041 cggccgggta ccgttttccg cgtgatcgtc gatggcatct ccgtgcgcca cgcggaccgc
     5101 ggcgccgcgc gcgtcgaggg cgatctgccc gtgctcgtcc tgcacggctg gggagcgcat
     5161 ctggaggcca tcgagccgat cgtcgcggcc ctggcgggcg agacgcgcgt gctcgcgctc
     5221 gacctcccgg gcttcggcca gagcgacccg ccgcccgagc cgtgggacgc cgacggctac
     5281 atgcgcttcg tgctgcgctt cctcgacgag ctgggcgtcg agcgcgtgca cgtggtgggc
     5341 cactcgcacg gcggccgcgt ggccatctgc atggcggccg agcaccccga gcgcgtcgcg
     5401 cgcctgctgc tcgtggacgc cgccggggtg ccacccaagc gcggcatgaa gtaccgcgcg
     5461 aaggtgtcgg tcgcgaaggc gggcaaggtg gcggggaggg tcggcgcctc cggcctccag
     5521 gacaggctcc gcgcgcgcgt cggctcgacc gactacctga acgcctccga ggagatgcgc
     5581 gcgacgttcc ggctcgtgat cggccaggac atgaccgagc gcctgccgcg catcggggcc
     5641 acgacgctgc tcgtctgggg cgaccaggac gacgacacgc cgctctggat ggcccaccgg
     5701 atggaggagc tgatcccgga cgccgggctc gccgtgttcg agggcgccgg ccactactcc
     5761 tacgcggacg accctgcgcg cttcggcagc gtcgcgcgcc tgttcctgtg cgagcagcca
     5821 cgcgcgctcg cggcgggggc gccggccgcg tgatcgccac cgggttcgac ctcgcgttcg
     5881 ccggactgtt cggcctgatg gcgggccgcc tccacgtgct gctgcacctg ttccagctcg
     5941 agcactacga gcccgcgcgc ctgcgcgtgt gggtgaagcg ccgcggcctg cggctcgacc
     6001 cgaacgagct cggatacctg accgggttcg ccgggctgat ggtggccgcc accgggttcg
     6061 acctgccagg cgtggtcctg gggctcggcg cgctcgccgc gatcgtgggc gtgcggcatg
     6121 gagcgagcgt gctgcggcgc gaccagatca agccgctggt cttcacgccg cgcgcgcggc
     6181 gcatgtacgc gaccgcactc ggcctcctcc tgctcgcggc gatcgcgatc tcggtcggcg
     6241 cgatcgtggg cgaccgcgag agcctcgccg cgaacctggg cctgctgctc gcgctgggct
     6301 cgtacgttct cgcgcccgag ctcctgatcc tcgcgaacct gctcgtcaag ccggtccagg
     6361 cgctcgacaa ccgccgcttc gtcgcgcgtg cgcgcaggaa gctcgccgag gtggacccac
     6421 tcgtggtcgg catcaccggc tcgtacggga agaccaccac gaagagctgc gtggccgccg
     6481 tcgccgagct gcgcgggccg gcttacccca cgcccgcctc gttcaacagc ttcctgggcg
     6541 tggtgcgcgc gatcaacgag ggactccagt cgcgccaccg gacgttcgtg gtcgagatgg
     6601 gcgcctaccg ccgcggcgac gtggccgagc tgtgcgagct cgcggggccg aagatcggga
     6661 tcctcaccgc gatcggcccc gcgcacctgg agcgcttcgg gtcgctcgac gagaccgagc
     6721 gcgcgaaggg ggagctgggc gagtcgctcc cggccgacgg cgcgctgatc acgcgcgcgg
     6781 acgacgagcg ctgccgccgg gcggcgcagc ggtccccggc gcccgttcgc ctgttcgcac
     6841 cgttcccgca cgtggacgcg gacgtgtggg ccgaggacgt cgagctgcgc gacggccgca
     6901 ccgtgttccg gctctgcgcg cgcgacgccg acgccgtcac ggtcaaggcg cggctgctcg
     6961 gcgagcacaa cgtggccaat ctcgtggccg ccgccgcggc cgggctcgag atgggcctcg
     7021 ggctcgacgc gatcggccgc gcgctcgcca aggtgcagcc gccggcgcac cggctcgccc
     7081 cgatcgtcaa cgcgagcgcc ggcgtggtcg tgatcgacga cgcctacaac tcgaacccgc
     7141 agggcgcggc ggccgcgctc gacgtgctcg cctcccaccc ggccgaccgg cgcctgctcg
     7201 tcacgccggg gatggtcgag ctgggcgatc ggcaggagga ggagaaccgc cgctttggcg
     7261 agcgcgccgc ggccgtctgc gacctcgtcg tgctggtcgg cccggagcag acgcggccgg
     7321 tgcgcgcggg cctcgaggcc gccgggttcg ccgactccgc cctccacgtg gtgcccgacg
     7381 cgggcgcggc ccaggagctg ctggcgcgca ccacgcgccg cggcgacgtg gtgctgttcg
     7441 agaacgacct gcccgacctc tacgcgtccg ggaacgggtc cggcggctga gtacagtgcc
     7501 gcgcatgaga gtcgccgtct gcttcggcgg cccgtcggtg gagcacgacg tctcgatcat
     7561 cagcgcccag cagctgatgg cggcgctcga ggaccgccac gagccggtcc ccatctacct
     7621 cgcccgcgac ggtcgctggt ggacgggcga ttcgctgcgc gagatcgggt cgtacgggtc
     7681 cgaccggccg accggcgccg agccggtgga gctgcgtctg ggcagctcgg gcgcgccgtt
     7741 cgcggtgccg gcggcctcgc gcttccgcgg tggccgcgac gtgccggtgg acgtggcgat
     7801 caacgccgtc cacggcaccg gcggcgagga cggcgccctg ctgggcgcgc tggagcagac
     7861 cgacgtgccc tacgccggcg gcggggtcgg gcccgcggcg gccgcaatgg acaaggccct
     7921 ggcgaagctc gtgttccgcg acgccggcgt ggacgtgaac ccgcacctcc aggtcacgcg
     7981 cgagcgcttc gcgggcgatc cggcgggcgc cgcggccgag gccgtcgagg ccgtggggct
     8041 gccctgcttc gcgaagccgt ccacgctcgg ctcctcgatc ggcgtcacgc gctgcgccga
     8101 cgccgccgag ctcgaggagg cgctggagct ctgcttcgag ctcgaccgcc gcgcgctggt
     8161 ggagccgtcg ctcgaggagg cgatcgagat caacgccgcc gtgctcggcc gccccggcgg
     8221 cgagctgcgc gtgtcggtgc tggagcagcc cgtgaagggc gacgccgcac tctcgttcga
     8281 ggacaagtac ctgcgcgccg gcgggaaggg cggcgcgaag gacggcgcgc ccggctccgc
     8341 gccctccgga ccgaaggcgg gctcctccgc gggcgccaag gccggcggcg gcgacggcgc
     8401 cggcatggcc agcgccgacc ggatcatccc cgcgccgatc gacgaggagc tggccgaccg
     8461 gatccgccag gccgcgctgg ccgcgcaccg cgcgctcggc ttcgccggcg tggtgcgcta
     8521 cgacttcttc gtgcacgagg accgcgtgat cctgaacgag gcgaacacgg tccccggctc
     8581 gttcgcgttc tacctctacg agccggatgg cctgaccttc cccgacctcg ccgacgcgct
     8641 ggtcgagatc gcgctggccg aggccgcgga gcgccgcgcc accacccgca ccttcgagtc
     8701 ggtgctgctc agccggcacc agggcggcgg ctgatgccgg gccgggtcgt ggtggcgagc
     8761 ggcggccgct cgctcgagcg cgagatcagc ctgcgctccg ggcgccgcgc gaccgacgcg
     8821 ctcgaggcgc tcggctacga cgcgatcccg atcgaccccg accacggctt cgtcggcgag
     8881 atcaagcgcc tgaggccgga gttcgtgttc gtggcgatgc acggtcgcgg cggcgaggac
     8941 ggcacgctcc aggacctgct cgagatcctc cacgtgccgt acacggggtc ggacgtgcac
     9001 gcgtccgcgc gctgcctcga caagcacgcg ttcaaggagc tgatggtcgg gatcggcctg
     9061 gccacgccgg tctggcactc gttcaaccgc gacgcgttcg ccgccttcgg cgccgccgac
     9121 acgctgcccg agctgatcgc gcagctcggc ttccccctcg tggtcaagcc tgcgcgcgag
     9181 ggatcgtcgc tcgggatcaa gttcgtgaac tcgagcgagg agttctcgca ggccgtggtc
     9241 ggcgcgctca gctacgacga ccgcgtggtg atcgagcact tcgtggaggg ccgcgagctg
     9301 gcggccaccg tgctcggccc gcccgacgcc cccgaggtgc tgccggtggt cgagatcaag
     9361 accgacgagc ccttctacac gttccgcgcg cactacgacg ctggcgtggc ggagctggag
     9421 gtggcgtcgc tcgagccggg cgtccaggag tccgtcgaga gcgccgcccg caaggcatac
     9481 accgcggccg gctgccgcga cttcgcgcgc gtggacatca tcctcgacac agagaacgag
     9541 ccccagatcc tggagatcaa cacgatcccg ggcctgacgg agacgggccc cgcgcgcttc
     9601 gccgccgagg ccgcgggact cgacttcccg gcgctggtgg agcgggtggt cgcgcgcgcg
     9661 gcgggcgagg cgggaggcgc atgatcggcc gctcgagccg ctcctagcgg tctaccccgc
     9721 aagcggtgtg tcagacctgt aacgctgcgt tcgcctcagg ctcgatctgg gccatgttgt
     9781 cgtcgtatag cgacccaaac atggcccagt agccccgggg ccgccacgcc gtgcggccgg
     9841 acgttacggg agggttacga gtgtttcagc cggagtcgcg tgcggccgcg agctccgcgc
     9901 ggtactcgtt ccggtacccc acgaccttgc ggacgtacgc gcgcgtctcg cggtagggcg
     9961 ggatgccgcg gtaccggatc accgcccgcg ggccggcgtt gtaggcggcg agcgagagct
    10021 ggatcttccc ccggaagcgg tcgagctgcg agcgcaggta gcgcgcgccg gcgtccagcg
    10081 agcgggcggg cacgatccgg tcgtcgacgc ccctcttgag gtccaccctc aggccgaggg
    10141 agcgcgccgt cgacggcatg agctgcgcga gcccgcgcgc gccggagcgg ctcgtggcct
    10201 cggggtcgaa gttcgactcg gcgtggacga gcgcggccag cagcaggcgg tccacgccgt
    10261 tggcgtcggc ggccgtggcg atctcgttcg cgaacgggac ggccggcatc ccctcgaccg
    10321 gcgcctgcgc gccggagggc tgcccgggcg gagcggctcc gccggtcggc tgctcctcct
    10381 gagcggcggc aggggcggcc agcgccagcg tcagcacgac ggcggcggct gtggcgagac
    10441 gacccatgcg ggctgcgacc ttcagcgaaa tcccctctcg tcttctgcgc ctacgaggtg
    10501 agctgacggg cttgcgctgt gcgctacgcc gctctcagac ggcgattcgc cccaaacgtc
    10561 ctggttcccc cgttccccac caccgacggc ggggactcgg cacgcccgga gtgtaaccct
    10621 gcgcgcggcg cgtgaaaccc gcggcgcacg acgtggtgct cacgacgctg gtcgagcgac
    10681 ctcccgctgc gttgagatag ggtgccgccc cgggcggggg agagggagag gatgccgcat
    10741 ctattcaggt cgcgaccggt cggattgcag gttgttctcg cggggatcgt ccccgcgctg
    10801 ttcggcgcgc tcacgggctg ggtgctcggg ctcagcgaga tcggctacct cgtgctctcg
    10861 ctcctcgctc tcgccggtgc gtacgcggcc gggctggagc acgacggcgc gggcgaggcc
    10921 gcgctgcgcg gagccctcgg cggcgcgctg ttcggtggct tcatcctcat cgtgcacaac
    10981 gcgaccgggg aagaggcgaa ggcggagctg cccgatcccg agatccttct catcgcggtc
    11041 acgatcgcgt tcagcgtggg cgccggtgcg ctcggcggcc ggcgccgtca caggcatgag
    11101 gccgaggggc ccaaggacaa gggggagaag gaggccgtga acttcaagcg actgcattgg
    11161 gccgagttca tcggcttcgc gggtgtgggc gtcctgctcg catcgatgtt cctgccgtgg
    11221 ttctccacga gctgcgacag cctgaggccg gcgacgccgg acggctgcaa cccgaactcg
    11281 accctcaacg ggctgcgcgg cgacttcaac gccttcgaga cctacaagat catggacatc
    11341 ctgctcgtcc tggcgtgcat cgcgccgttc gtgctcgcct acctggtcat ccgcgagtcg
    11401 cagctctcgt ggcgcccggg cgaggtcacg atgatcgtcg ggatggtcgc cgcggcgctg
    11461 atctggctga acgggatcat cctcggccgg ccgggcgaca gcgtcgacat cgggatcgag
    11521 atcggctact ggatcgggct gatcggggcg aacatgatcc tcgccggcgg gctcatacgc
    11581 caggccctcg gcgggcgcac gcgcaaaccg cccggaacgc tctagccgtg cccgatccca
    11641 cgggcgcgcg tcccgaccgc aacctggcga tggagctcgt cagggtcacc gagtacgggg
    11701 ccctggccgc ggcgcgctgg gtcggactgg gggagaagga gaaggccgac ggcgcggccg
    11761 tggacgcgat gcgcctgatg ctcgacaccg tgcggatgga cggcgtcgtc gtcatcggcg
    11821 agggcgagaa ggacgaggcg ccgatgctct acaacggcga gcagatcggc gacggctcgc
    11881 cgccgcaggt ggacatcgcg gtcgatccgc tcgagggcac gacgctcgcc gcggagggat
    11941 cgccgcgcgc gctcgcggtc atggcgatgt ccgagcgcgg gtcgatgttc aacccggggc
    12001 ccgccgtgta catgctgaag atcgccgcgc agccggcgtt cgcgcacctg ctcgacctcg
    12061 accggccgct gagcgagacg ctcgagctga tcgcgcgcga gaagggcacc gacgtccgcg
    12121 acgtgaccgt gatgatgctc aagcgcgacc gccacaacga ggccgtcgag gagatccgcc
    12181 gcgcgggcgc gcggatccgg ttcatctcgc acggggacgt ctcggcctcg ctgctcgcgg
    12241 tccaggacga gtcgccgatc gacctgctct gggggatcgg cggcacgccc gagggcgtga
    12301 tctcggcggc cgcgatcaag tgcctcggcg ggcagatgct cgggcgggtc tggccgcgca
    12361 gcgacgagga gcgccaggcg atcgccgact tcgggctcga cctcgacgag gtgctcgacg
    12421 tggaccgcct gatcgcgggc gacgacgtgt tcttctccgc cacgggcgtg accgacggcg
    12481 acgtgctcga gggcgtgcac tacacgacca cgggcgcgac gacggagtcg ctcgtcatgc
    12541 gcacgcgctc ggggacggtg cggcgggtct cggcggtgca cgatcgcgcc aagctgcgcg
    12601 agatcagcgg gcagcgcgcc ggctagctct caggcgctct ggacctgctc ggcgcgctgc
    12661 tcggccacgg ggtccgcggc cgggttctca cccgccagcg ccttccgcgc ctcctcgcgc
    12721 agcgagccga ctacagactc cggcagctcg agccgagcca gcgagcgcag gaacgcgttg
    12781 gtcacgatca cgtggatgcg cagctgggtc tgctggtaga gccaggcgcc caggcgcgcg
    12841 agcttgcgcg gtacccagct ctgcgcgatg gcggggtaga agttcgccac ctccgagctc
    12901 acgcgcgcgt tgatctcgtc gccgcccggg tcgggctgtg gccgctcgac gcagacgcgc
    12961 aggtggccct tgttgcgccc ctgcggtgcg accaggattc ccttctcgat ccgccaggtg
    13021 acggtgccgc ggtgggggag ggtctcgtac tcgggcgggc ggaaggtgag gagccgcagg
    13081 ggcctcgcga cgagcacgac ctcgcgaccc tcgggcgtgt agagcacgcg gatcaggccg
    13141 agcgtgaagc ggctgaggaa gacccagtag gtgcgcgcga ggcgctcgag gtactcggcc
    13201 cgccagagct tgtcgagctc cgcgcgcggg agggtgacgt cggccacctg cttcgtggtc
    13261 acgctgccgt cctcggtcga ggagccctgc ggcgtcgcga cgatgttcac gcgcgcgacc
    13321 gcgcggcggc ggcgctcgag cagtcgtctc gggtctctcg gtctcacccc tgaaacgtac
    13381 ccagcgggaa cggctcggcg tccacgaggg cgccgggtgc gagctcgccc gtgccgcggg
    13441 ggatgagggc cagggcgccg gcgccgagca tcgagctgat ccggtgggat ccctgggcgc
    13501 cggtgggctc ggcgctccag ccgtcgtcgc tcgcggtcag gcggcagcgg atggcctgct
    13561 cgcgccgctc gctccgggtc acgggagccg tgagtcgcgc gcgcacgcgt gcgggggcgg
    13621 ggtcggcgcc ggccagcgcg cggatggccg ggtgggcgaa gagctggaac gtgaccatcg
    13681 cggagaccgg gttgccgggc agaccgaaca cgagcgcgcc ggttggggtt gccccgaacc
    13741 aggtcggctt gcccggcttg agcgcgacgc cccagaagat ttgctcgacg ccgagctccg
    13801 cgagagccgg cttcacgtgg tcgtgctcgc cgaccgagac gccgccggac ccacacacca
    13861 cgtccgcctt gtcgagcgcg gcgcgcagcg ccgcgacggt ggcggcgtgg ctgtcgccga
    13921 cccgccggtg gccgtccacg cgtgcgccgg aggcggtggc gagggccacg agcgccggtc
    13981 cgttcgagtc gcggatctgg ccggggccaa gcggcgcgcc gggatcgacc agctcgtcgc
    14041 ccgcgaccac gacggccacg cgcggcaccg cgccgcagcg gacctcggtg cgcccgagcg
    14101 cagcgagcat cgccagctcg gacgggccga tctgcgcgcc ccggcgcagc acgacctcgc
    14161 cggcccgcac gtcctcgccg gcacggcgga tgttggcgcc gggcgcgagg gtc
//
LOCUS       DASVPL010000032         5367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_198843, whole genome shotgun sequence.
ACCESSION   DASVPL010000032 DASVPL010000000
VERSION     DASVPL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198843"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VF517_01705"
     CDS             complement(<1..568)
                     /locus_tag="VF517_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8051679.1"
                     /translation="MNRKRTPTPAWTLTLTAIASLMVVLDALVVSTALDSVRADLGAS
                     IEDLEWTVNAYVLTFAVLLMTGSAIGDRFGRRRTFAGGLALFAAASAACALAPTVGWL
                     IAARVLQGVGAAFVMPLALALVSAAYPPAARPRALGVFASVGGLAVALGPLLGGAVIE
                     GISWPWIFWLNVPAALVLIPLSLTRIEES"
     gene            658..1308
                     /locus_tag="VF517_01710"
     CDS             658..1308
                     /locus_tag="VF517_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX8051680.1"
                     /translation="MATPRTYGDRCAAARALDLVGQRWALLVVRELLLGAKRFTDLAE
                     GMPNAKPSVLSQRLRELEQGGVVQRRTLGPPARVSVYELTDMGRELEPVVIALGRWGR
                     RLPMPTGAAHGIDSLVLALKWRFNPEAAGDLSATYGLRMAGDGFRIGVADGRMEVQRG
                     EPDEPDSVIETDPRTLERVIFDGGDAAEAERSGELRLAGDRALATRLLKLYAPARS"
     gene            1393..2811
                     /locus_tag="VF517_01715"
     CDS             1393..2811
                     /locus_tag="VF517_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8051681.1"
                     /translation="MKATTRWIVVLTGIGSLMAALDTLVMSTALSTIRADLGASIEQL
                     EWTVNAYNLSFAVLLITGAALGDRFGRRRLFAAGLGLFAVASAACALAPSVGWLIAAR
                     AVQGAGSALLMPLGLALLSAAFPPEKRGAAIGIFSGITGLAVASGPLVGGAVIQGISW
                     EWIFWINVPIGVIAVPLVLTKMKESFGPDTALDLTGLGLITAAALGIVWGLVRGNSAG
                     WDSVEVIATIAVGVLLVAAFVAWERRAPAPMLPLELFRSRPFSAGNAAIFFTFAALFG
                     AVFFAAQLMQTGLGYDAWGAGLRLLPWTGTFMTIAPIAGAMADRIGERPLMVSGLVLQ
                     SVGMAWLALVAEPGVAYGSLLWPLLVAGVGVSLAIPAAQNSVLGSSAMDAIGKAAGVN
                     SMMRELGGVFGIAVAVAVFAGAGSYVSADAFFDGFGPAFGVIAGLSALGAMASMALPG
                     RRSTAAFDAVRPIPAFEADGGA"
     gene            2861..3217
                     /locus_tag="VF517_01720"
     CDS             2861..3217
                     /locus_tag="VF517_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051682.1"
                     /translation="MHLELHTRDLAAASAFYGELLHWRTEEITVRCRSYHALTLGGDL
                     DGGIVECGAPYGTWLPYVQVEEIEAMTERGRRLGASVLLEPREGPTGWRSVLATRDAG
                     DIALWQPKRGPRLEAV"
     gene            3214..4209
                     /locus_tag="VF517_01725"
     CDS             3214..4209
                     /locus_tag="VF517_01725"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8051683.1"
                     /translation="MTEERLLAAAAAGDETAYAELVEPHRRPLHAHCYRMLGSVQDAE
                     DAVQETLLRAWRSLGSFEGRSSFRSWLYTIATNVCLRTVERRQRRVLPVDYGAPADPH
                     DRLGAPLSETTWIEPYPEERFVLEDGLAGPEARYEQRESIELAFIAALQHLPPRQRAV
                     LILRDVLGFSGAEVAEALETTPASVYSILQRAHDAVDRRLPELSQQATLRSLGDGELR
                     EIVDRYVTAWEQHDVDAITSMLTDGATLAMPPTPTWYRGRAAIETGLRRGPLDGVRRW
                     SLSATYANGQAAAAASIWNDDEGRFVLHHTAILTLRGARIEAIDAFHPPAALRAA"
     gene            complement(4346..4948)
                     /locus_tag="VF517_01730"
     CDS             complement(4346..4948)
                     /locus_tag="VF517_01730"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX8051684.1"
                     /translation="MRNGGQIDIDYDAGPQPGGREPAPGRLALVQAFVNSRYDLELDH
                     GADLLGDPAGLRAWLARRGLLDGRATVTRRDLARALAVREGLHAMVVSNNGGVLDEDA
                     VQRLNETAARAPFGARFELAGPVLVPSARGVDGALAVILAATTEAMLAETWWRLKACQ
                     HHDCQWAFFDHSRNAAGNWCSMKVCGGRAKQRAHYRRTRR"
     gene            5089..>5367
                     /locus_tag="VF517_01735"
     CDS             5089..>5367
                     /locus_tag="VF517_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051685.1"
                     /translation="MMKTVLVTGANRGLGLATVRRLAASDTTVLVGARDIHSGERAAA
                     AIRADGNDAVAVELDVLSTESLARAAAAIDRRYGRLDLLVNNAGILPEA"
BASE COUNT          743 a         1920 c         1964 g          740 t
ORIGIN      
        1 ggctctcctc gatccgggtg aggctcagcg ggatgaggac cagcgccgcc ggcacgttga
       61 gccagaagat ccacggccat gagatcccct cgatgacggc gcccccgagc agcgggccga
      121 gcgccaccgc caggccgccc acgctcgcga acacaccgag cgcccggggg cgcgccgcgg
      181 gcgggtacgc ggcgctcacg agcgccaggg cgagcggcat cacgaaggcc gcaccgacgc
      241 cctggagcac acgcgccgcg atcagccagc cgacggtcgg cgccagcgcg caggccgcgg
      301 acgccgccgc gaacagggcg agaccccccg cgaacgtccg ccggcgtccg aagcggtcgc
      361 cgatcgcgga gcccgtcatc agcagcaccg cgaaggtgag gacgtaggcg ttgaccgtcc
      421 actcgaggtc ctcgatggag gcgccgagat cagcccgcac cgagtcgagt gccgtcgaca
      481 cgaccagcgc atcgagcacg accatcagcg aggcgatcgc ggtcagcgtc agcgtccaag
      541 ccggagtcgg cgtcctcttc ctgttcatcc ggtccccttc gtcatcgctt ggttgcgcaa
      601 cactagcgat cgatagtaat taacaagtag gtgatagcac gacgctagag tcggaccatg
      661 gcgacacccc gcacatacgg cgatcggtgc gctgccgcgc gtgcgctcga cctggtcggg
      721 cagcgctggg cgctcctcgt ggtgcgcgag ctgctgctcg gcgccaagcg cttcacggac
      781 ctggcggaag ggatgcccaa cgccaagccg agcgtgctgt cgcagcgcct gcgggagctc
      841 gagcagggcg gcgtcgtaca gcgccgcacg ctcgggccgc ccgcgcgcgt gtcggtctac
      901 gagctcaccg atatgggcag ggagctcgag ccggtcgtga tcgcgctcgg ccgctggggc
      961 aggcgcctgc cgatgccgac cggcgccgcg cacggcatcg actcgctcgt cctcgccctg
     1021 aagtggcgat tcaatcccga ggccgccggc gatctgagcg ccacctacgg gttgcgcatg
     1081 gccggggacg gcttccggat cggcgtcgcg gacgggcgga tggaggtcca gcgcggcgag
     1141 ccggacgagc cggacagcgt catcgagacc gacccgcgga cgctcgagcg agtgatcttc
     1201 gacggcggtg acgcggccga ggcggagcgc tccggggagt tgcgcctcgc gggcgaccgc
     1261 gcgctcgcga cccgcctcct gaagctctac gcgccggcga ggtcctgacc cacccgcgca
     1321 cccgaccgat ggatttcgcc gcccgcccgt atctcagttc aggacaacgg acggcgagcg
     1381 aagggcgggg cgatgaaggc gacaacgcgg tggatcgtgg tgctgaccgg catcggatcc
     1441 ctgatggcgg cactggacac gctggtgatg tcgacggcgc tgagcacgat ccgggccgac
     1501 ctgggcgcct cgatcgaaca gctggaatgg acggtcaatg cctacaacct gagcttcgcg
     1561 gtgctgctga tcacgggcgc ggcgctcggc gaccgcttcg ggcgccggcg gctgttcgcc
     1621 gcgggcctcg gcctgttcgc ggttgcgtcc gcggcgtgcg cgctcgctcc ctccgtgggc
     1681 tggctgatcg ccgcgcgggc ggtgcaggga gccgggtccg ctctgctgat gccgctggga
     1741 ctggcgttgc tgagcgctgc gttcccgccg gagaagcgag gcgccgcgat cgggatcttc
     1801 agtgggatca cgggcctggc ggtcgcgagc ggcccgctcg tcggcggcgc cgtgatccag
     1861 ggaatcagct gggagtggat cttctggatc aacgtgccga tcggggtgat cgccgtcccg
     1921 ctcgtgctga ccaagatgaa ggagagcttc ggcccggaca ccgctctcga cctcacggga
     1981 ctcgggctga tcaccgcggc ggcgctcgga atcgtgtggg gcctcgttcg tggcaactcc
     2041 gccggctggg acagcgtcga ggtgatcgcg acgatcgccg tgggcgtgct tctcgtcgcg
     2101 gcgttcgtcg cctgggagcg gcgcgcgccc gcgccgatgc tgccgctcga gctcttccgc
     2161 tcacggccgt tctcggccgg caacgccgcg atcttcttca ccttcgcggc cctgttcgga
     2221 gcggtgttct tcgccgctca gctgatgcag accgggctcg gctacgacgc ctggggcgcc
     2281 ggcctgcgac tgctgccgtg gaccgggacg ttcatgacga tcgcaccgat cgcgggcgcg
     2341 atggccgatc ggatcggcga gcggccgctg atggtcagcg gcctggtcct gcagtcggtc
     2401 ggaatggcct ggctcgcgct cgtcgccgag cccggggtcg cgtacgggtc gctgctctgg
     2461 ccgctgctcg tcgcgggcgt cggggtctcg ctggcgatcc ccgccgcgca gaactcggtc
     2521 ctcggctcgt cggcgatgga cgcgatcggc aaggccgcgg gcgtcaacag catgatgcgc
     2581 gagctcggag gcgtcttcgg catcgccgtc gccgtggcgg tgttcgccgg ggcggggagc
     2641 tacgtgtcgg cggacgcctt cttcgacggc ttcggcccgg cgttcggcgt catcgccggc
     2701 ctgtcggcgc tcggcgcgat ggcgagcatg gcgctgcccg gccggcgctc gaccgccgcg
     2761 ttcgacgcgg tccggccgat ccccgcgttc gaggcggacg gcggcgcatg agcgccgccg
     2821 tcgccctgcg gcacacggga ccggcaatat gagaccggtg gtgcacctcg aactgcacac
     2881 tcgcgacctg gccgccgcca gcgcgttcta cggcgagctg ctgcactggc ggaccgagga
     2941 gatcaccgtt cgctgccgct cctaccacgc actcacgctc ggcggcgacc tcgacggcgg
     3001 gatcgtcgaa tgcggtgctc catacgggac gtggcttccg tacgtgcagg tcgaggagat
     3061 cgaggcgatg accgagcggg ggcgccggct cggcgcgtcg gtgctgctcg agccgcgcga
     3121 gggcccgacg ggctggcgca gcgtgctggc cacccgcgac gcgggcgaca tcgcgctctg
     3181 gcagccgaag cgcggaccga ggctggaggc ggtatgaccg aggaacgcct gctcgcggcc
     3241 gccgcggccg gggacgagac cgcctacgcc gagctggtcg agccgcaccg ccgtccgctc
     3301 cacgcgcatt gctaccggat gctcggctcg gttcaggacg ccgaggacgc cgtgcaggag
     3361 acgctgctga gggcctggcg cagcctgggg tcgttcgagg ggcgcagctc gttccgctcg
     3421 tggctctaca cgatcgccac caacgtgtgc ctgcgcacgg tcgagcggcg ccagcggcgg
     3481 gtgctcccgg tcgactacgg cgcgccggcc gacccgcacg accggctcgg agcgccgctc
     3541 tcggaaacca cctggatcga gccgtacccc gaggagcggt tcgtgctcga ggacgggctc
     3601 gccgggcctg aggcccgcta cgagcagcgc gagagcatcg agctggcctt catcgccgcg
     3661 cttcagcatc taccgccccg ccagcgggcc gtcctgatcc tgcgcgacgt gctcgggttc
     3721 tcgggcgcgg aggtcgccga ggcactcgag acgaccccgg cgtcggtgta cagcatcctc
     3781 cagcgcgcgc acgacgccgt cgatcgccgc ctgccggagc tgagccagca ggccacgctg
     3841 cgcagcctcg gcgacggcga gctgcgcgag atcgtcgacc gctacgtcac cgcctgggag
     3901 cagcacgacg tcgacgcgat cacctcgatg ctgaccgacg gcgcaacgct cgcgatgccg
     3961 ccgacgccga cctggtaccg cggccgcgcc gccatcgaga ccggcctcag gagaggaccg
     4021 ctcgacggcg tgcggcgctg gagcctgagc gcgacctacg ccaacggcca ggccgcggcc
     4081 gcggccagca tctggaacga cgacgagggc cgcttcgtgc tgcaccacac cgcgatcctc
     4141 acgcttcgcg gcgcgcggat cgaagccatc gacgcgttcc accccccggc ggcgctgcgc
     4201 gcggcctaac gctcggccgg ccggcggcta cgcccagcgc tctccggcgg ctcccttcac
     4261 gagagccgga gcacgatgcg cccgtggtgg agggccgtgc tgcgtcggag gcgcaggtca
     4321 cggagcgccc cgatagccgc gctcgtcacc ggcgcgtccg gcggtaatgc gcgcgctgct
     4381 tcgcgcggcc gccgcagacc ttcatcgagc accagttgcc cgcggcgttg cgcgagtggt
     4441 cgaagaaggc ccactggcaa tcgtggtgct ggcacgcctt caggcgccac catgtctcgg
     4501 cgagcatcgc ctcggtggtg gccgcgagga tcaccgcgag cgcaccgtcg acgccccgcg
     4561 cactgggcac cagcaccggg cccgcgagct cgaagcgggc tccgaaggga gctcgggcgg
     4621 cggtttcgtt gagccgctgg acggcgtcct cgtcgagcac accgccgttg ttcgacacga
     4681 ccatcgcgtg cagcccctcg cgaaccgcga gcgcgcgcgc gaggtcccgg cgcgtcacgg
     4741 tcgcgcgacc gtcgagcagg ccgcgtcgcg cgagccaggc cctcagcccg gccggatctc
     4801 cgaggaggtc cgcgccgtgg tcgagctcga ggtcgtagcg cgagttgacg aaggcctgga
     4861 cgagcgcgag gcgccccggc gccggttcgc gcccgcccgg ttgcggaccg gcgtcgtagt
     4921 cgatgtcaat ctgaccaccg ttacgcattt cgatggttag attacttcgc gtgcccgcgc
     4981 ggtactggag acgccgcgcg ggccgcgacg cgcgggcggc ggcgaacctc acatatccac
     5041 ggcggcgcgc gtttaggggg caataaaccc cagcaggagg ccacaccgat gatgaagaca
     5101 gtgctcgtga ccggcgccaa cagagggctc gggctggcga ccgtgcggcg gctggccgca
     5161 tccgacacca cggtgctcgt gggtgcccgc gacatccaca gcggcgagcg cgccgccgcg
     5221 gcgatccgtg ccgacggcaa cgacgccgtc gcggtggagc tcgacgtcct gtcgacggag
     5281 tcgctggcgc gcgcggccgc cgcgatcgac cgccgctacg gccggctcga cctcctcgtc
     5341 aacaacgcgg gcatccttcc cgaggcg
//
LOCUS       DASVPL010000033         7413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_120773, whole genome shotgun sequence.
ACCESSION   DASVPL010000033 DASVPL010000000
VERSION     DASVPL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120773"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..622)
                     /gene="pyrE"
                     /locus_tag="VF517_01740"
     CDS             complement(68..622)
                     /gene="pyrE"
                     /locus_tag="VF517_01740"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX8051686.1"
                     /translation="MSARADLVAELREHALVLGEFTLTSGATSTYLIDAKRAILRPAG
                     FRALAQLVAAEATAVGAEAVGGMTMGADPIACAAIAGGAELPAFFVRKERKEHGLSRW
                     IEGPPLEPGTRCLIVEDVVTTGGSTIRAIERVRDEGHEICGVLVILDRLAGGDEAIRE
                     AAGREVPYTALATIDDVYPDRPDK"
     gene            complement(619..978)
                     /locus_tag="VF517_01745"
     CDS             complement(619..978)
                     /locus_tag="VF517_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051687.1"
                     /translation="MSFLVDPPWLFANGRAYARLAPEEAQGGLARAAGAACLAAYWGA
                     GVSLYLNAPYTKPLWPLLPGRDGRDFMVNWPGLRVPTRRRTRRLDAAAAAVMLGVYPL
                     ALWLGWDHGRRARPRTP"
     gene            1003..1836
                     /locus_tag="VF517_01750"
     CDS             1003..1836
                     /locus_tag="VF517_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8051688.1"
                     /translation="MPPDPFNIARGAGALANARRSLLPLPLVDRPGPIPRDLFPPPAQ
                     RPALTSGLGVAAATSGGAAVALTGTARAFEDAGVRPAAISVSSAAVLWGAMWAAGMSA
                     EEMADYSLGWRPEAALGVQWTGLPRLAVSAVRGFGGLARGAALEQLFPRHVWHMEAGA
                     TEVPLHAIAYDLDRDRLVPLGSEATPELTLGELARVAITPPRRADAVRIEGRYYADGG
                     ALDPLAARAKLTASGIEDVVELPAGGGGFYDLFLDRRRWPDHIRDGYAVTRRRRSESP
                     S"
     gene            complement(1802..2812)
                     /locus_tag="VF517_01755"
     CDS             complement(1802..2812)
                     /locus_tag="VF517_01755"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8051689.1"
                     /translation="MRTRSLLFTLLLALAVAAPAHAQVPGVGEPSTQPAPAPPAPTAQ
                     PAAAKLSVGVASMGDRGKRWVLAGESLVASGRLDPFVAGEKVSVELYKGKKRLGTRVV
                     EVKDDKGHGAFTASFGAVKTAGSYFVIAKHAASAGQAGATSERARFGAVRAASGSGSS
                     GQHVRLMQLGLRRLAYVTPVNGRHDGATGRAVMAFRKVNGMARTFSANRAIFRALFAR
                     RGGYKLRFPKAGRHAEADLSRAVLVLAEGGKPVRIYHTSPGKPSTPTVRGSFRVYRRQ
                     PGTNSHGMVHSAYFIRGYAVHGYNSVPAYPASHGCLRVPIPDARSIYNWFTMGTRIDV
                     YA"
     gene            complement(2881..3762)
                     /locus_tag="VF517_01760"
     CDS             complement(2881..3762)
                     /locus_tag="VF517_01760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX8051690.1"
                     /translation="MGHPAPPYGPQYGHGYAFPHPPPPDPPELPAGARAWPRWPWWYG
                     PTAMAVGFVVTLITVGILGAILVAAGTEDIEDAKGFLQIATVLQQIIFVSVAVFFASL
                     TVRPRAWQFGLRRSRFWPTLGWAALGFATYWVLAIAYSAAVQPDAEQETLESLGTDES
                     TLFLVGAAVLVIVLAPLAEEIFFRGFFYRALRTRLPIVAAALVDGAIFGAIHFENADM
                     AVILPVLAILGTIFCLVYEKTGTLFATIGLHAFNNFIAFGVETEEWAVAGVVGGLMIA
                     ACMTVPRLLPARTPAPA"
     gene            complement(3771..4430)
                     /locus_tag="VF517_01765"
     CDS             complement(3771..4430)
                     /locus_tag="VF517_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX8051691.1"
                     /translation="MRAVLLDSLGTLVELEPPVPRLHERLRDLGFRVDEELVAHAFGA
                     EVAYYVEHHVEGRDQSTLAELRNRCAAVLLDALGIPELPLPDARDALLASLSFRAFPD
                     AAPALRDLRERGLRLVVVSNWDCSLREVLDSAGLLELVDDAVSSAEAGAAKPDPAPFH
                     AALAAAGCEPGEAVHVGDSEENDVAGARAAGVRAVLLRRAETPGEPGAIASLSELAAV
                     L"
     gene            4457..5257
                     /locus_tag="VF517_01770"
     CDS             4457..5257
                     /locus_tag="VF517_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX8051692.1"
                     /translation="MRAEITALVTVALAFGAAQLVDRAVARREEPLAAAVPGGGSAAA
                     GTRLRLLRRLVYALILVIGIALALSQFEGVRRVATGVLASSAVLGLVVGFAARATLAN
                     VVAGVLLAITQPIRIGDSVTFEGSTGTVEDIRLTYTHIAMDDGTRVIVPNERLAQNTV
                     ANHTIGSGPARVEVALYLAPDADPIRALAVLGREEGVDVRVDEVEKDAIRIVAGATAP
                     SRAERAALEAGLRVGCLERLMREGLSSTGPPRAPSRHDPSPKKASPAS"
     gene            5220..7406
                     /locus_tag="VF517_01775"
     CDS             5220..7406
                     /locus_tag="VF517_01775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8051693.1"
                     /translation="MTHRQRKLRRRRSRTGRNGAFLAVLIVLSAILIAGLSGVGYVFA
                     VAASAPDIDGLKPIDKGASSIIYAADGSRLGYVQSDTIRTPVGWSKIPPIVRQATVAI
                     EDERFYRHKGVDVQAVGRAAFEDVVAQRSKQGGSTITQQLVRQLYIERPDRNIQRKIK
                     EAKMAADLEQERSKQWILREYLNVIPYGTVNGKTAIGIQAAAQLFFSRKAQDLTLTQA
                     AMLAGLPQAPSQYNPLRNPTDALERRNRVLEKMAEHGYITQAEATTASSEPLGLKRST
                     IFDKRREPYFFDYVQDKLIERYGVGVYRRGGLKIHTTIDPRAQEQGRAAINGRLGQPG
                     DPSSAIVAIDPKTGYIRAMASSGTYNDRTFNLAAQGHRQPGSAFKTMVLVTALRKGID
                     PNSTVYVSKPLQLNVPGYGPWKVKTYDGSYGGAMNLVQATIKSDNTVYAQLDIDVGPK
                     EVAETAHMLGIRTKLDGIPSEGLGGLRLGVSPLEMSSAYATLAAGGMYSEPKAIRRVE
                     FPDGKSDDLGKPKRKRVISDGVAYEVTKILQQNVQRGTGTRAQIGCPAAGKTGTTDNY
                     NDAWFAGYTPSLAAAVWVGYPNALKEMRNVHGIQVAGGTFPTEIWGDFIRGVKRDCRS
                     FPPPKEPARFSTFFGRNSASGRAGGGYRAPTYGGGGGSNSAGGGNYRGYDPRLYTEPP
                     QAPPRTTPPPSGGGNKGGGNGNGGGNDGGGNGGADGGNGGGAGADG"
BASE COUNT         1076 a         2711 c         2709 g          917 t
ORIGIN      
        1 acgcggcgag cctcaacacg ctgctgacga gcctgcgcgg taggcggccg ctgctctagc
       61 ggacgcgcta cttatccgga cggtccggat acacgtcgtc gatcgtcgcc agcgccgtgt
      121 acgggacctc gcgcccggcc gcctcgcgga tcgcctcgtc gccgcccgcg agccggtcga
      181 ggatcacgag cacgccgcag atctcgtggc cctcgtcgcg cacccgctcg atcgcgcgga
      241 tggtcgagcc gccggtcgtg accacgtcct ccacgatcag gcagcgcgtg cccggctcga
      301 gcggcggccc ctcgatccag cgcgacaggc cgtgctcctt gcgctccttg cggacgaaga
      361 aggccggcag ctcggcgccg ccggcgatag cggcgcatgc gatcgggtcg gcgcccatgg
      421 tcatcccgcc gacggcctcg gcgccgaccg ccgtcgcctc cgccgccacg agctgggcga
      481 gcgcgcggaa gccggccggc cggaggatcg cgcgcttggc gtcgatcagg taggtggacg
      541 tcgcgccgct cgtcagcgtg aactcgccga gcaccagggc gtgctcgcgc agctccgcga
      601 cgagatccgc gcgtgcactc acggcgtgcg cgggcgcgcg cgccgcccgt ggtcccaccc
      661 gagccacagc gcgagcgggt agacgccgag catgaccgcg gccgccgccg cgtcgagccg
      721 tcgcgtgcgc cgcctggtcg gcacccgcag cccgggccag ttgaccatga agtcgcgccc
      781 gtcgcggccg gggagcaggg gccagagcgg cttggtgtac ggcgcgttca ggtagagcga
      841 gacgcccgcg ccccagtagg ccgcgaggca ggcggcgccg gccgcgcggg cgaggccgcc
      901 ctgcgcctcc tcgggcgcca gccgcgcgta cgcgcggccg ttcgcgaaca gccagggagg
      961 atcgacgagg aacgacacgg cgcgcgtaat ctacccgcgc ccatgccccc cgacccgttc
     1021 aacatcgcgc gcggcgccgg cgcgctggcc aacgcccggc ggagcctcct cccgttgccg
     1081 ctggtggacc ggccggggcc gatcccgcgc gacctcttcc ccccgcccgc gcagcggccc
     1141 gccctgacca gcggcctcgg ggtggcggcc gcgacctccg gcggcgcggc ggtcgcgctc
     1201 accggcaccg cgcgcgcgtt cgaggacgcg ggcgtgcggc cggcggcgat ctcggtgtcc
     1261 tcggccgcgg tgctctgggg tgcgatgtgg gccgcgggca tgagcgcgga ggagatggcc
     1321 gactactcgc tcggctggcg tccggaggcg gcgctcggcg tgcagtggac cggcctcccg
     1381 cgactcgcgg tgtccgcggt gcggggcttc ggcggcctcg cgcgcggcgc cgcgctcgag
     1441 cagctcttcc cccgtcacgt ctggcacatg gaggcgggcg ccacggaggt ccccctgcac
     1501 gcgatcgcgt acgacctcga ccgcgaccgg ctcgtccccc tgggaagcga ggcgacaccg
     1561 gagctgacgc tgggggagct ggcgcgcgtg gcgatcacgc caccgcgccg cgccgacgcg
     1621 gtgcgcatcg agggccgcta ctacgccgac ggcggcgcgc tcgatccgct ggccgcccga
     1681 gcgaagctga ccgcgtcagg catcgaggac gtggtcgagc tgcccgccgg cgggggcggc
     1741 ttctacgacc tcttcctcga ccggcgccgc tggccggacc acatccgcga cggctacgcc
     1801 gttacgcgta gacgtcgatc cgagtcccca tcgtgaacca gttgtagatc gagcgggcgt
     1861 cggggatcgg gacgcgcagg cacccgtggc tcgccgggta ggcgggcacc gagttgtagc
     1921 cgtgcaccgc gtagccgcgg atgaagtacg ccgagtggac catcccgtgc gagttcgtgc
     1981 ccggctggcg gcggtagacg cggaacgagc cgcgcaccgt cggcgtcgac ggcttgcccg
     2041 gcgaggtgtg gtagatccgc accggctttc cgccctccgc gaggacgagc accgcgcgcg
     2101 agagatcggc ctcggcgtgc cggcccgcct tggggaagcg cagcttgtag ccgccgcgcc
     2161 tggcgaacag cgcgcggaag atcgcgcggt tggccgagaa cgtccgggcc atcccgttga
     2221 ccttgcggaa cgccatgacc gcgcgcccgg tggcaccgtc gtgcctgccg ttgacgggcg
     2281 tgacgtacgc gagccggcgc agcccgagct gcatcagccg cacgtgctga ccgctcgacc
     2341 cggacccgga ggcggcgcgc accgctccga agcgcgcgcg ctcggaggtg gcgccggcct
     2401 ggccggcgct ggccgcgtgc ttggcgataa cgaagtagga accggctgtc ttgaccgcgc
     2461 cgaagctggc ggtgaacgcc ccgtggccct tgtcgtcctt gacctcgacg acgcgggtgc
     2521 cgagccgctt cttgcccttg tagagctcga cgctgacctt ctcgcccgcg acgaacgggt
     2581 cgagccggcc ggacgcgacg agcgactcgc cggcgagcac ccagcgcttg ccgcggtcgc
     2641 ccatcgatgc cacgccgacc gacagcttgg ccgcggcggg ctgcgcggtg ggcgcgggcg
     2701 gcgcgggcgc gggctgggtc gagggctccc cgacgccggg cacctgggcg tgtgccgggg
     2761 cggcgacggc gagggcgagc agcagggtga agagcagcga gcgagttctc atatgggttc
     2821 cattctgtcc tatgtcgttg gctcctaccc ctgcctggcg cggatgcact tgcgcggcac
     2881 tcacgcgggg gcgggggtgc gcgcgggaag gaggcgcggc acggtcatgc aggcggcgat
     2941 catcaaaccg ccgacgacgc ccgcgacggc ccactcctcg gtctcgaccc cgaacgcgat
     3001 gaagttgttg aaggcgtgca ggccgatcgt cgcgaacagc gtgccggtct tctcgtacac
     3061 caggcagaag atcgtcccga ggatcgcgag caccgggagg atcacggcca tgtcggcgtt
     3121 ctcgaagtgg atcgccccga agatcgcgcc gtcgacgagc gcggcggcca cgatcggcag
     3181 cctcgtgcgc agcgcgcggt agaagaagcc gcggaagaag atctcctccg ccagcggcgc
     3241 gagcacgatc acgagcacgg cggcgccgac caggaagagc gtgctctcgt cggtgccgag
     3301 gctctcgagc gtctcctgct cggcgtccgg ctgcaccgcc gcgctgtagg cgatcgccag
     3361 gacccagtac gtcgcgaagc ccagggcggc ccagccgagg gtgggccaga agcggctgcg
     3421 ccgcaggccg aactgccacg cgcgcgggcg cacggtgagc gacgcgaaga agacggcgac
     3481 gctcacgaag atgatctgct ggaggaccgt cgcgatctgg aggaagccct tggcgtcctc
     3541 gatgtcctcg gtgcccgccg cgaccaggat cgcgccgagg atcccgaccg tgatcagcgt
     3601 gaccacgaag ccgaccgcca tggctgtcgg cccgtaccac cacggccagc gcggccaggc
     3661 gcgcgcgccc gcgggcagct cgggcgggtc cggcggcggc gggtgcggga aggcgtagcc
     3721 gtggccgtac tgcgggccgt acggaggcgc gggatgaccc atcgtcggac ctagagtacg
     3781 gccgcgagct cgctcaggct cgcgatcgcg cccggttccc cgggcgtttc ggcgcggcgc
     3841 agcaggacgg cgcggactcc ggcggcccgc gcgccggcga cgtcgttctc ctcggagtcg
     3901 cccacgtgca cggcctcgcc gggctcgcaa ccggccgccg cgagcgccgc gtggaacggc
     3961 gccgggtcgg gcttggccgc gccggcctct gccgacgaga cggcgtcgtc cacgagctcg
     4021 agcaggccgg ccgagtccag cacctcgcgc agcgagcaat cccagttgct cacgaccacg
     4081 agccgcagcc cgcgctcgcg caggtcgcgc agcgccggcg cggcgtccgg gaaggcccgg
     4141 aacgagagcg acgccaggag cgcgtcgcgc gcatcgggca gcggcagctc ggggatcccg
     4201 agcgcgtcga gcagcaccgc ggcacagcgg ttgcgcagct cggcgagcgt gctctggtcg
     4261 cgaccctcga cgtggtgctc gacgtagtac gccacctcgg cgccgaacgc gtgcgccacg
     4321 agctcctcgt ccaccctgaa gccgaggtcg cgcaggcgct cgtgcagacg cggcacgggc
     4381 ggctcgagct cgacgagcgt gccgagcgag tcgagcagta cggcgcgcat ccggacccca
     4441 ccgtaccctg cgcggcgtgc gggcggagat cacggctctc gtcacggtcg ccctcgcgtt
     4501 cggggccgct cagctcgtcg atcgcgcggt cgcgcgccgc gaggagccgc tcgcggcggc
     4561 ggtccccggc ggcggctcgg cggcggccgg cacccgcctg cggctcctga ggcggctggt
     4621 ctacgcgctg atcctcgtga tcgggatcgc gctggcgctc agccagttcg agggcgtccg
     4681 gcgcgtggcc acgggcgtgc tcgcgtcgtc cgcggtgctc ggcctcgtcg tcggcttcgc
     4741 cgcgcgcgcg acgctcgcga acgtcgtcgc cggggtgctg ctcgcgatca cccagccgat
     4801 ccgcatcggc gactcggtca cgttcgaggg cagcaccggc accgtcgagg acattcgcct
     4861 gacctacacc cacatcgcga tggacgacgg cacgcgcgtg atcgttccga acgagcggct
     4921 cgcgcagaac acggtggcca accacacgat cgggtcgggc cccgcgcgcg tggaggtggc
     4981 gctctacctg gctcccgacg cggacccgat ccgcgcgctc gcggtgctcg gtcgcgagga
     5041 gggcgtggac gtgcgcgtcg acgaggtgga gaaggacgcg atacggatcg tggccggcgc
     5101 gaccgccccg agccgcgccg agcgggccgc tctcgaggca ggactacgag tcggttgcct
     5161 cgaacgactg atgcgcgagg gactatcctc gaccgggccc ccgcgggccc cctcgcgcca
     5221 tgacccatcg ccaaagaaag cttcgccggc gtcgtagccg gaccggccgc aacggcgcgt
     5281 tcctggccgt cctgatcgtc ctgagcgcaa tcctgatcgc cgggctctcc ggcgtcggct
     5341 acgtgttcgc ggtggccgcc tcggcccccg acatcgacgg cctcaagccg atcgacaagg
     5401 gcgccagctc gatcatctac gcggcggacg gctcgcggct cggatacgtc cagtcggaca
     5461 cgatccgcac gccggtcggg tggtcgaaga tcccgccgat cgtgcggcag gcgacggtcg
     5521 ccatcgagga cgagcgcttc taccgccaca agggcgtgga cgtgcaggcg gtcggacgcg
     5581 cggcgttcga ggacgtggtc gcgcagcgct ccaagcaggg cggttcgacg atcacccagc
     5641 agctcgtccg ccagctctac atcgagcgtc cggatcgcaa catccagcgc aagatcaagg
     5701 aggccaagat ggccgccgac ctcgagcagg agcgcagcaa gcagtggatc ctgcgcgagt
     5761 acctgaacgt catcccgtat ggcaccgtca acggcaagac cgcgatcggc atccaggccg
     5821 ccgcgcagct cttcttctcg cgcaaggccc aggacctcac gctcacgcag gcggcgatgc
     5881 ttgccgggct gccgcaggcg ccgtcgcagt acaacccgct gcgcaacccg accgacgcgc
     5941 tcgagcgccg caaccgggtg ctcgagaaga tggccgagca cggctacatc acccaggccg
     6001 aggccacgac cgcctcgtcc gagccgctcg ggctcaagcg cagcacgatc ttcgacaagc
     6061 gccgcgagcc gtacttcttc gactacgtgc aggacaagct catcgagcgc tacggcgtgg
     6121 gcgtctatcg ccgcggcggc ctgaagatcc acacgacgat cgacccgcgc gcgcaggagc
     6181 aggggcgcgc cgcgatcaac ggccggctcg gccagcccgg cgacccgagc tcggcgatcg
     6241 tggcgatcga cccgaagacc ggctacatcc gcgcgatggc gtcgagtggc acgtacaacg
     6301 accgcacctt caacctcgcc gcgcagggcc accgccagcc gggctcggcg ttcaagacga
     6361 tggtgctcgt cacggcgctg cgcaagggga tcgaccccaa ctcgaccgtg tacgtctcga
     6421 agccgcttca gctcaacgtg ccgggctacg gcccgtggaa ggtgaagacc tacgacggca
     6481 gctacggcgg cgcgatgaac ctcgtccagg cgacgatcaa gtccgacaac acggtttacg
     6541 cgcagctcga catcgacgtc gggccgaagg aggtcgccga aacggcgcac atgcttggca
     6601 tccgcacgaa gctcgacggc atcccgtccg aggggctcgg cggcctgcgc ctcggcgtgt
     6661 cgccgctcga gatgtcgagc gcctacgcca ccctcgccgc gggcggcatg tacagcgagc
     6721 cgaaggcgat cagacgggtc gagttccccg acgggaagtc cgacgacctc ggcaagccca
     6781 agcgcaagcg cgtgatcagc gacggcgtgg cgtacgaggt cacgaagatc ctccagcaga
     6841 acgtgcagcg cggcaccggc acgcgcgcgc agatcggctg ccccgccgcc ggcaagaccg
     6901 gcacgaccga caactacaac gacgcctggt tcgcgggcta cacgccgtcg ttggccgcgg
     6961 ccgtgtgggt cggctacccg aacgcgctga aggagatgcg caacgtgcac ggcatccagg
     7021 tggcgggtgg cacgttcccg accgagatct ggggcgactt catccgcggc gtcaagcgcg
     7081 actgccgctc gttcccgccg ccgaaggagc ccgctcgctt cagcaccttc ttcggccgca
     7141 actcggcctc gggccgcgcc ggcggcgggt accgcgcgcc gacctacggc ggtggcggcg
     7201 gcagcaactc ggccggcggc ggcaactacc gcggctacga cccgcgtctg tacacggagc
     7261 cgccgcaggc gcccccgcgc acgaccccgc ccccgagcgg cggtggcaac aagggcggcg
     7321 gcaacggcaa tggcggtggc aacgacggcg gcggtaacgg cggggccgac ggcggcaacg
     7381 gcggaggcgc aggcgccgac gggtaggtcc tgc
//
LOCUS       DASVPL010000034         6995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_4199, whole genome shotgun sequence.
ACCESSION   DASVPL010000034 DASVPL010000000
VERSION     DASVPL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4199"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VF517_01780"
     CDS             complement(<1..1245)
                     /locus_tag="VF517_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8051694.1"
                     /translation="MMRIGVIGLGYVGLPLGVAFAEAGHEVMGLDTDPRRIDAVTNGE
                     SYIEDIASHRLKALDGRLRATARYADLSKCDAVIIAVPTPLTENREPDLGALMASGES
                     LAGVLQEGQLVVLESTTYPGTTRERLVPLLEKSGLKAGRDFNVAFSPERVDPGRTDYT
                     LRTTPKVLGGLTPDCLERARAMYREVCDKLVEVSTLEAAELSKLLENVFRSVNIALVN
                     ELAMLCDRMEIDIWEVVDAAATKPYGFMRFDPGPGMGGHCLPVDPFYLAWKAREYDTP
                     TEFIELAGEVNQRMPYFCVEKISRALNDHSKPVRGSRIAILGVSYKAGVGDVRESPGL
                     KIVTLLAALGADLRYHDPFVPDLPELGLSSRPFDEVIADADLALIVTAHPGVDHDAVV
                     AQVPLALDLRGVTRRSGGRAVLL"
     gene            1327..2331
                     /locus_tag="VF517_01785"
     CDS             1327..2331
                     /locus_tag="VF517_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8051695.1"
                     /translation="MPPTAPAPETALDRAEELAESILPTRPPKRRMLVIVNPYASTVS
                     DRLKNLVVYALQGRYEVDAIDTQAKAHATELCREAADEGYDVVVAFGGDGTVNEAANG
                     LAGSDTPLSALPGGSTNVFCRSLGIPNDVVDATEHLLGMADRFEPSRVDLGVANDRFF
                     TFAAGVGLDASVVEQVDRHPAMKARLSEWYFTGTALTTFAQKYLIRAPRVTVEAGGHE
                     IEGVTVIVQNSDPYTYFSSHPIRVCIGAGNRTGTLSAAVLKRATPFEVPTIIARIFAG
                     GPDSVSKHAQVEPLTDLRELRIVSKDGRRFPLQLDGDHVGDVTEVELGVRHLALPVVS
                     "
     gene            complement(2328..4586)
                     /locus_tag="VF517_01790"
     CDS             complement(2328..4586)
                     /locus_tag="VF517_01790"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX8051696.1"
                     /translation="MATTDRSTAVRPQVELDQFERNLLADLFAVIEEHEESGFPHLER
                     EPIERAFVFACERHADQRRRSGEDFIVHPVAVAKICSEMRLDTATVCAALLHDTVEDT
                     SASLDEVREEFGEEIATLVDGVTKLTGITFQSRDEKQAENYRKMIVAMAHDIRVVLIK
                     LADRLHNMRTLEAMPKQKQQETAKETLEIFAPLAHRLGIHAIKSELEDLAFVTLHPRK
                     FKEIKGLVNQQRTERERYVSRAGDYLRKELEEVGIEAEISGRAKHFYSIYSKMTKKGR
                     EFNEIYDLTAMRVLVGSVKDCYGAIGVIHSLWKPLPGRFKDWVAMPKFNMYQALHTTV
                     IGPEGRPLEIQIRTGDMHETAEYGIAAHWTYKDGNGGKGSGGTERIQWIEQLIDWQKD
                     LKDPQDFVEGLKVDLFEDEVFVFTPKGEVKSLAAGATPLDFAYEIHTDVGHRCVGAKV
                     NGKIVPLHYELKSGDICEVLTSKKERGPSRDWLSLAKTTRAQSKIRAWFKRERREDAE
                     RTGREILAESLKRAGLPAQKMSGSPLLADVIREMGFKKGEDFYIALGQAKVSAKVVTS
                     KLLTRLKQGEAAVEAPPGDELITHRRKEVAKPVQSSSNYGIRVQGIDDVPLRLAKCCR
                     PVPGDDVVGYISLGKGITIHRGDCPNARALKRNPERFTPVEWEGEGATSFRVELQIDG
                     WDRTRMLEDLSRTFAESGINILEARCTVDHPMVKNRFVVEVGDTHALKTCIQRLRNVD
                     SVFDAYRVTPAG"
     gene            4667..5674
                     /locus_tag="VF517_01795"
     CDS             4667..5674
                     /locus_tag="VF517_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8051697.1"
                     /translation="MSDGPVQVGVVGLGYWGPNIARNFDRLAESELRWICDSREEAVE
                     RFAPQLPGSRASTRLEDLLEDPELDAVAITTPVPTHAELALRVIEAGKHCFVEKPLAQ
                     SVEAAEEVTAAAERAGVVLMVGHLLEYHPGFERLKEIADAGELGEIHYLYSNRLNLGK
                     LRADENALWSLGAHDVSVVLRLAGEEPSECRAVGESYMRPGVEDVVFCYLRFPSGLAA
                     HLHLSWLDPHKDRRFTVVGSRRMATFDDMELERKLTVYDKGFDQDYSSYGEYIARSGD
                     VWSPKISNEEPLRIECRHFLERVRDGAEPRSGGRSGVRVVRVLQELQRSLEESSRAAR
                     V"
     gene            5658..6329
                     /locus_tag="VF517_01800"
     CDS             5658..6329
                     /locus_tag="VF517_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX8051698.1"
                     /translation="MLRESDRAPGLVLGDGALLPQSAEVGAHVVIHDGTEIGEFARIQ
                     DGAVLGKPVALGARSSGSREPVPPLRIGRNVQIGAGAVIVAGAELADGVTVADQAHVR
                     ERARIGHESLVGRGSAVDCDVTIGAGVRIQTNCYITALSEVEDDVFVGPGVSTLNDRA
                     AGRRPEGEPLRGVVLRRGCRIGGGSVVLPGVEVGADAFVGAGAVVTRDVPPGVLVVGV
                     PARAV"
     gene            complement(6326..>6995)
                     /locus_tag="VF517_01805"
     CDS             complement(6326..>6995)
                     /locus_tag="VF517_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8051699.1"
                     /translation="PELRELGLPVLEDAAQAAGATLDGRCAGSLGDAATFSFFPSKNL
                     PCLGDGGAIVTDDDAVAETARTLRFHGSADKETFTEVGYNSRLDEAQAAALRVLLPEL
                     DGWNEARRAAAAAYDEAGLREVEGLRVLDSAPGAVHHLFVIAHERPDELAAALAERGV
                     QARGYYRRPVHEQPAMAPYATSADLPGTDELARTNLALPMGPLLTTEQAREVVAALRG
                     AAAS"
BASE COUNT          946 a         2394 c         2564 g         1091 t
ORIGIN      
        1 cagcagcacc gcgcggccac ccgagcggcg cgtgaccccg cgcaggtcga gcgcgagcgg
       61 cacctgtgcg acgaccgcgt catggtcgac gccggggtgc gcggtgacga tcagcgcgag
      121 gtcggcgtcg gcgatcacct cgtcgaacgg gcggctggac aggccgagct ccggcaggtc
      181 gggcacgaac gggtcgtggt agcgcaggtc ggcgccgagc gcggccagca acgtcacgat
      241 cttcagcccc ggcgactcgc gcacgtcgcc gacgccggcc ttgtaggaca cgccgaggat
      301 cgcgatccgc gagccgcgca ccggcttcga gtggtcgttc agcgcgcgcg agatcttctc
      361 gacgcagaag tagggcatcc gctggttgac ctcgccggcg agctcgatga actcggtcgg
      421 cgtgtcgtac tcgcgcgcct tccaggcgag gtagaacggg tcgaccggca ggcagtggcc
      481 gcccatgccg gggccggggt cgaaccgcat gaagccgtac ggcttggtcg cggccgcgtc
      541 gacgacctcc cagatgtcga tctccatgcg gtcgcagagc atcgccagct cgttgaccag
      601 ggcgatgttg accgagcgga agacgttctc gaggagcttg ctgagctccg cggcctcgag
      661 cgtcgagacc tcgacgagct tgtcgcacac ctcgcggtac atggcgcggg cgcgctcgag
      721 gcagtccggc gtgaggccgc cgagcacctt cggggtcgtg cgcagcgtgt agtcggtccg
      781 gccggggtcc acacgctccg gcgagaacgc gacgttgaag tcgcggccgg ccttcaggcc
      841 cgacttctcg aggagcggta cgagccgctc gcgcgtggtg ccggggtagg tcgtcgactc
      901 gagcacgacc agctggccct cctggaggac gccggcgagc gactcgccgg aggccatgag
      961 ggcgccgagg tccggctcgc ggttctcggt gagcggggtg ggcacggcga tgatcaccgc
     1021 gtcgcacttg gagaggtcgg cgtagcgggc ggtggcgcgc aggcgcccgt cgagcgcctt
     1081 cagccggtgc gaggcgatgt cctcgatgta gctctcgccg ttggtcacgg cgtcgatgcg
     1141 gcggggatcg gtgtcgagcc ccatcacctc gtgccccgcc tcggcgaacg cgacccccag
     1201 cgggagcccc acataaccga gccctatcac cccgatgcgc atcatcgcca gactagtgtc
     1261 ctcggcagtc ggcggcgttc ctgaacggcc agtaacgact tgggcgcggc ggggttgtca
     1321 cctcccatgc ccccgacggc ccccgcgccc gagacggcgc tcgaccgggc tgaggagctc
     1381 gccgagagca tccttccgac ccgcccgccc aagcggcgca tgctcgtgat cgtgaacccg
     1441 tacgcgagca cggtgtcgga ccgcctgaag aacctcgtgg tctacgcact ccagggccgc
     1501 tacgaggtcg acgccatcga cacccaggcc aaggcccacg ccaccgagct gtgccgggag
     1561 gccgccgacg agggctacga cgtggtggtc gccttcggcg gcgacggcac cgtcaacgag
     1621 gccgccaacg gcctcgccgg ctcggatacg ccgctgtcgg ccctgcccgg gggctccacg
     1681 aacgtcttct gccgcagcct cgggatcccc aacgacgtgg tcgacgccac cgagcacctg
     1741 ctcggcatgg cggaccgctt cgagccctcg cgcgtggacc tcggggtggc gaacgaccgc
     1801 ttcttcacct tcgccgcggg cgtgggcctc gacgccagcg tggtcgagca ggtggaccgc
     1861 cacccggcca tgaaggcgcg cctcagcgag tggtacttca ccggcaccgc gctcacgacc
     1921 ttcgcgcaga agtacctgat ccgcgcgccg cgggtgaccg tcgaggcggg cgggcacgag
     1981 atcgagggtg tcacggtgat cgtgcagaac tccgacccct acacgtactt ctcgagccac
     2041 ccgatccgcg tgtgcatcgg cgccggcaac cggaccggaa cgctgtccgc cgcggtgctg
     2101 aagcgcgcca cgccgttcga ggtccccacg atcatcgcgc ggatcttcgc cggggggccc
     2161 gactcggtct cgaagcacgc ccaggtggag ccgctgaccg acctgcgcga gctgcggatc
     2221 gtgtcgaagg acggccgccg cttcccgctc cagctcgacg gcgaccacgt gggcgacgtc
     2281 accgaggtcg agctcggtgt gcgccacctg gcgctgcctg tcgtctccta gccggccggc
     2341 gtgacgcggt aggcgtcgaa caccgagtcc acgttacgca ggcgctggat gcaggtcttc
     2401 agcgcgtggg tgtcccccac ctcgaccacg aagcggttct tcaccatcgg gtggtcgacg
     2461 gtgcagcgcg cctcgaggat gttgatgccg ctctcggcga acgtgcgcga gaggtcctcg
     2521 agcatgcggg tgcggtccca gccgtcgatc tggagctcga cccggaacga ggtcgcgccc
     2581 tccccctccc actccaccgg cgtgaagcgc tccgggttgc gcttgagcgc ccgcgcgttg
     2641 gggcagtcac cgcggtggat cgtgatgccc ttgccgagcg agatgtagcc gaccacgtcg
     2701 tcgccgggca ccggccggca gcacttcgcc agccgcagcg gcacgtcgtc gatcccctgc
     2761 acgcggatgc cgtagttcga cgagctctgg accggcttcg cgacctcctt gcggcggtgc
     2821 gtgatcagct cgtcgccggg cggggcctcg acggccgcct cgccctgctt gaggcgcgtg
     2881 agcagcttgc tcgtgacgac ctttgccgag accttcgcct ggccgagcgc gatgtagaag
     2941 tcctcgccct tcttgaagcc catctcgcgg atcacgtccg ccagcagcgg ggagccggac
     3001 atcttctgcg ccgggagccc ggcgcgcttg aggctctcgg cgaggatctc gcggccagtt
     3061 cgctcggcgt cctcgcggcg ctcgcgcttg aaccacgcgc ggatcttcga ctgggcgcgc
     3121 gtggtcttgg cgagcgacag ccaatcgcgt gaggggccgc gctccttctt cgaggtgagc
     3181 acctcgcaga tgtcgccgga cttcagctcg tagtgcagcg ggacgatctt cccgttcacc
     3241 ttcgcgccga cgcagcggtg cccgacgtcg gtgtggatct cgtaggcgaa gtcgagcggc
     3301 gtggcgcccg cggcgagcga cttgacctcg cccttgggcg tgaagacgaa gacctcgtcc
     3361 tcgaacaggt ccaccttcag cccctccaca aagtcctgcg ggtccttcag gtccttctgc
     3421 cagtcgatga gctgctcgat ccactggatc cgctcggtcc ccccggatcc cttgccgccg
     3481 ttgccgtcct tgtaggtcca atgcgcggcg atgccgtact cggcggtctc gtgcatgtcg
     3541 cccgtgcgga tctggatctc gagcgggcgc ccctcgggcc cgatcacggt ggtgtggagc
     3601 gcctggtaca tgttgaactt gggcatcgct acccagtcct tgaagcgccc cggcagcggc
     3661 ttccagagcg agtggatcac gccgatcgcg ccgtagcagt ccttcaccga cccgacgagc
     3721 acccgcatgg ccgtgaggtc gtagatctcg ttgaactcgc ggcccttctt ggtcatcttc
     3781 gagtagatcg agtagaagtg cttggcgcgg cctgagatct cggcctcgat cccgacctcc
     3841 tcgagctcct tgcgcaggta gtcgccggcc cgcgagacgt agcgctcgcg ctcggtgcgc
     3901 tgctggttca cgaggccctt gatctccttg aacttgcgcg ggtggagcgt gacgaaggcg
     3961 aggtcctcga gctcggactt gatcgcgtgg atgccgagcc ggtgggccag cggcgcgaag
     4021 atctcgagcg tctccttggc cgtctcctgc tgcttctgct tgggcatcgc ctcgagcgtg
     4081 cgcatgttgt ggaggcggtc ggcgagcttg atgagcacga cgcggatgtc gtgcgccatc
     4141 gcgacgatca tcttccggta gttctcggcc tgcttctcgt cccggctctg gaaggtgatc
     4201 ccggtgagct tcgtgacgcc gtccaccagg gtcgcgatct cctcgccgaa ctcctcgcgc
     4261 acctcgtcca gcgaggcgct cgtgtcctcg actgtgtcgt gcagcagcgc ggcgcacacg
     4321 gtggccgtgt cgagccgcat ctcggagcag atcttcgcca ccgcgacggg gtggacgatg
     4381 aagtcttcgc cggagcgccg gcgctggtcg gcgtggcgct cgcaggcgaa cacgaaggcg
     4441 cgctcgatgg gctcgcgctc gaggtgcggg aagccgctct cctcgtgctc ctcgatgacg
     4501 gcgaacagat cggccagcag gttgcgctcg aactgatcca gctcgacctg cggccggacg
     4561 gcggtggagc gatccgtagt cgccatgggt cgagtttagt cccgggagcc gaccccaaag
     4621 cgcagtcggc gcggcgaggt ctggcgcgcc tgttattcct tcctccgtga gcgacgggcc
     4681 cgtccaagtc ggcgtcgtgg gcctcggcta ctggggcccg aacatcgcgc gcaacttcga
     4741 tcggctggcg gagtcggagc tgcgctggat atgcgactcg cgcgaggagg cggtggagcg
     4801 gttcgcgccg cagcttccgg gctcgcgggc gagtacccgc ctggaggacc tgctcgagga
     4861 cccggagctc gacgccgtgg cgatcaccac cccggtgccg acccacgcgg aactcgccct
     4921 gcgggtgatc gaggccggca agcactgctt cgtggagaag ccgctggcgc agtcggtgga
     4981 ggccgcggag gaggtcacgg ccgcggccga gcgcgcgggc gtcgtgctga tggtgggcca
     5041 cctgctcgag taccacccgg ggttcgagcg gctcaaggag atcgccgacg ccggcgagct
     5101 cggcgagatc cactacctct actcgaacag gctgaacctc gggaagctgc gagcggacga
     5161 gaacgcgctc tggtcgctgg gcgcgcacga tgtgtcggtg gtgctgcggc tcgcaggcga
     5221 ggagccgagc gaatgccgcg cggtcggcga gagctacatg cggcccgggg tcgaggacgt
     5281 ggtgttctgc tacctgcgct tcccgtccgg gctcgcggcg cacctgcacc tgtcgtggct
     5341 cgacccgcac aaggaccggc gcttcaccgt ggtcggctcg cgccggatgg ccacgttcga
     5401 cgacatggag ctcgagcgaa agctgaccgt ctacgacaag gggttcgacc aggactactc
     5461 gtcgtacggc gagtacatcg cgcgctcggg cgacgtgtgg agcccgaaga tctcgaacga
     5521 ggagccgctg cggatcgagt gccggcactt cctcgagcgc gtccgcgacg gggcggagcc
     5581 gcgctccggc ggtcgcagcg gcgttcgcgt ggtcagggtg ctccaggagc tgcagcggtc
     5641 gctcgaggag tcgtcgcgtg ctgcgcgagt ctgatcgcgc gccgggcctg gtgctcggcg
     5701 acggggcgct gctgccgcag tcggccgagg tgggcgcgca cgtggtgatc cacgacggca
     5761 cggagatcgg cgagttcgcg cggatccagg acggggcggt gctgggcaag ccggtggcgc
     5821 tcggggcgcg gtcgagtggg tcgcgcgagc ctgtgccgcc gctcaggatc ggccggaacg
     5881 tgcagatcgg ggcgggcgcg gtgatcgtgg cgggcgccga gctggccgac ggggtgaccg
     5941 tggcagatca ggcgcacgtg cgcgagcgcg cgcgcatcgg gcatgagtcg ctcgtgggcc
     6001 gcgggtccgc cgtggactgc gacgtgacga tcggcgccgg ggtccggatc cagaccaact
     6061 gctacatcac ggcgttgagc gaggtcgagg acgacgtgtt cgtcggcccc ggtgtgagca
     6121 cgctcaacga ccgcgcggcg gggcgccggc cggagggcga gccgctgcgc ggcgtggtcc
     6181 tgcggcgcgg ttgccgcatc ggcggcgggt cggtggtgct gccgggcgtg gaggtcggcg
     6241 ccgacgcgtt cgtcggagcc ggcgccgtgg tcacgcgcga cgtgccgccc ggtgtgctcg
     6301 tggtgggcgt gccggcgcgc gcggtctagc tcgcggcggc tccgcgcagc gcggcgacga
     6361 cttcacgggc ctgctcggtc gtgagcagcg ggcccatcgg cagcgcgagg ttcgtccggg
     6421 ccagctcgtc ggtccccggc aggtcagcgc tggtcgcgta gggcgccatc gccggctgct
     6481 cgtggacggg gcggcggtag tagccgcgtg cctggacccc gcgctcggcc agcgccgcgg
     6541 ccagctcgtc gggccgctcg tgggcgatca cgaagaggtg gtgcacggct ccgggcgccg
     6601 agtcgagcac ccgcaggccc tcgacctcgc ggaggccggc ctcgtcgtag gcggccgcgg
     6661 cggcgcggcg ggcctcgttc cagccgtcga gctcgggcag caggacccgc agagcggcgg
     6721 cctgggcctc gtcgaggcgc gagttgtagc cgacctcggt gaaggtctcc ttgtcggccg
     6781 agccgtggaa gcgcagggtg cgggccgttt ccgccaccgc gtcgtcgtcg gtcacgatgg
     6841 cgccgccgtc gccgaggcag ggcaggttct tcgaggggaa gaacgagaac gtggcggcat
     6901 cccccaggct gcccgcgcag cggccgtcga gcgtcgcgcc cgcggcctgc gccgcgtcct
     6961 cgagcaccgg cagccccagc tcgcgcagct cgggc
//
LOCUS       DASVPL010000035        29601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_160420, whole genome shotgun sequence.
ACCESSION   DASVPL010000035 DASVPL010000000
VERSION     DASVPL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 29601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29601
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160420"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VF517_01810"
     CDS             <1..290
                     /locus_tag="VF517_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051700.1"
                     /translation="IRQEMVDAPRPCDAVRRWAGNGWSPSASPGDPRQGLAAAQAAER
                     SLLRVGRTLSALGVSPRAAQGMTPGGLIELAAPTIITCRSRRELTAAQGCS"
     gene            399..1136
                     /locus_tag="VF517_01815"
     CDS             399..1136
                     /locus_tag="VF517_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051701.1"
                     /translation="MHGAHIRRAFPPHVLAMVLLAGLVVAGVAVAAESLDRPFGFFSR
                     EPSEVLGAPFYTGFLFHVGLLVWFASAVACLFGGCLVAALRGRRAAAPLLAAGLLSTV
                     LALDDALRIHEDLLPGKLGLPKIGTYALYAVAFGAWLWFFRDFVRRTPWPLLGFAGLM
                     LGTSVGLDRVFESHQRHLFEDGAKFVGIVAWTAYFVRTSFASALEAVAARPVSDESKA
                     ALAEPAAAPARGERRFERRRDRQPLPR"
     gene            complement(1145..3220)
                     /locus_tag="VF517_01820"
     CDS             complement(1145..3220)
                     /locus_tag="VF517_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEX8051702.1"
                     /translation="MRKLLLPAVLVLPLLFASEASAVKTVCAAGCDATTVQGGVDLAA
                     AGETVTVGAGTYTEQVDVAKNGLVLQGAGVGQTIIRSPAGPVSKFTTDTLAPDNAPVV
                     WVHDVAGATVEDMTIDGAGRGGPTCTLNFHGLAFRRAGGTADDLAITGFRHTPLDGCQ
                     VGNGVYVFNDDAAPPARTLTLSDIAVSDFQKNGVSISSTNLAMSLSNVDVTGAGAQTD
                     IAQNGIVVSSAIVPQGSPGGTQPTGPVPTGTISASEVKNHLCNQASCGPDPQTQSQSS
                     GIALIDSNVDLTGNVVSNNDNGIRPRSSVLTRAGTSSISGNTIAGNRFAGVYATAGVA
                     TLTSNVILGPGNAGVRAGAFLQGGNQFLPTLTLVANHIGSTSAGIDLFRNSSSSASPA
                     IVAARNRILGNTAGVNNTTPGSVGAENNWWGCNAGPGAAGCDPTTGTVDSDPRLVLGA
                     SASPATIETGGKTSSIVASVRRNSAGAVQPSPFFPAVPSTFAATLGSIAPTATTIADG
                     QATGTLTSGTQTGTSTVTSTLDNQSATTDVAFVDPPVDPGPGPGPGPGPGPDPGPGPG
                     PVTAPEAKLVISHRRVPYAGGRIKLKLICRGGAGARCTGRVNLIATSATRRVSGAASS
                     SARFDVAAGSSQTVRVQPPRRMVQQIRQRRRAIGKAIARLDAISGQTLAARLVKRLIT
                     VQARRSKRR"
     gene            complement(3306..5099)
                     /locus_tag="VF517_01825"
     CDS             complement(3306..5099)
                     /locus_tag="VF517_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051703.1"
                     /translation="MNSHRLIPLALILLVAAVAPAANAATKPIAGQAPRAEAPAPADA
                     VRGLLEATRGQKARTGLGLPHYKALLGSRWESKVRKADSAFAAAIAAPAAGGLRKSAA
                     GFGGDPLGAADSIKGKNAKQHRRLRIETALDSLCPTHNPGVDADGYWEIKGRARGEHV
                     VTTVERVGRYDVTTSVILDVQFEVHSQTGGASMVGIFAEAGKVSITRSQTARDRRTGK
                     TRKTGPTQRFSESLSPLLILDGGFDEFVEQQDRDESPAPRRPLRSGVWSDLAQRFVSV
                     VYLAIERDFAGAERHFQTPNTCVDMTIPAPEFLAPGGKVNLHGVPRLKQGSATVDHLI
                     ANSPVLAQELTTQGESYRSLAGATPWKAGEDWIEFTAPPQTWPASNPAGVRLILKSGA
                     GVAEAVASFKPIEGTVYFKVLEVSGGVDATDVKTTSGQCTFTGGPQHVEISPPAQPGT
                     VPHGKLEEQFGTIWTPITYRRGAYSTLHTCPTSQTPCNYAPESIPNQNLGINLRNTGD
                     GRVKLSWSFVAVQLGNSACSLQVPDLYVNRDLLETTHPLGDFTGSKPFVVETSNSQTY
                     SVQQGISTMTGKLSWNFRMVIQRVRQDGSAL"
     gene            5254..5943
                     /locus_tag="VF517_01830"
     CDS             5254..5943
                     /locus_tag="VF517_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX8051704.1"
                     /translation="MARLGSKARALLITVLMAAAVGGGWAIGASSDMDEMESHDTGAP
                     ASAAEDDRDVERGFLQAMVPHHESGIEMAQMALEHSQAPVIRRLSQAIVDTQAREIEQ
                     MRKMHERLFGEPLEPDGDAHMALGLSAEEAGMAGMSHGGEMMRKLADAKPFDRAYVDD
                     MVPHHQGAVAMAEVLLERTDDAELKRLAQAIIDAQRREIATMNDFRTKEYGGPVPEKP
                     GDGGHGGMKME"
     gene            complement(6138..6773)
                     /locus_tag="VF517_01835"
     CDS             complement(6138..6773)
                     /locus_tag="VF517_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8051705.1"
                     /translation="MSAPAPVTVTPIRVADLLAEDERMPVNVHVIDHPDGRVLVDTGM
                     TELHPAVADMDPRLQPLGEQDLDLGSIDLVVSTHLHFDHCGGNHLFAGRPIYVQRQEL
                     DDALSQDDYTIREWVEAPGVRYEPVDGELELLPGLRLVPAPGHTRGMQMVVIETGERP
                     VIVGGDVAIRLGELDEPQSEGQLKVHALQPELVWLAHVDEPWMPTSAAAAH"
     gene            6877..8319
                     /locus_tag="VF517_01840"
     CDS             6877..8319
                     /locus_tag="VF517_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX8051706.1"
                     /translation="MAPKVRPVGRQEASRPIDLVIAIDRDAPATLGAQIESHFRRAVR
                     DGQLRAGTRIPSTRDLARQLSVSRRVTVDAYAQLAAEGYLVLRQGAHPQVADGAIATD
                     RERRDGPELAPEVLPRFDFRPSRPDVSAFPRQAWSRSLRTAVASIADAELIYGNPCGV
                     DPLRTTLSEYLGRVRGVVGAPGNVVVTSGYLQGLGLACRALAAGGARRIALEDPSAPE
                     QGPIARRAGLEPVPVPVDASGLRVDALDAAGVDAVVVTPAHQHPTGVVLAPERRTALV
                     DWLRRTDAFAIEDDYDAEYRYDRAAVKAFQGLDPGRVVYAGSTSKTLAPALRMGWLVV
                     PGRLLDAVAEEKFLADQGSARIEQHALADFIARGELDRHLRRMRLRYRARRDLLIDAL
                     ADQLPDAQVQGIAAGLHVTVRLRGTFDELALREAARARRVELSTLSDYTPGAFSRDPV
                     LLVGYAQVSEAAMAAGVRELAAVVASSRTG"
     gene            complement(8342..8412)
                     /locus_tag="VF517_01845"
     tRNA            complement(8342..8412)
                     /locus_tag="VF517_01845"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8378..8380),aa:Cys,seq:gca)
     gene            complement(8501..8574)
                     /locus_tag="VF517_01850"
     tRNA            complement(8501..8574)
                     /locus_tag="VF517_01850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8538..8540),aa:Gly,seq:gcc)
     gene            complement(8662..9075)
                     /locus_tag="VF517_01855"
     CDS             complement(8662..9075)
                     /locus_tag="VF517_01855"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX8051707.1"
                     /translation="MAIESVVVHVDGGSRGNPGPSAVAAVASSPDGDPLLERSRYIGE
                     TTNNVAEYKAVQLGLELARELGASAVEVVNDSQLIARQIGGQYKVKHAGLKPLFLETM
                     RALREFDKWSVRDVRREHNKRADELVNEELDRAGR"
     gene            complement(9078..9302)
                     /locus_tag="VF517_01860"
     CDS             complement(9078..9302)
                     /locus_tag="VF517_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051708.1"
                     /translation="MTIYCVIPEALEAELYDRMVEHYKDDPDVEVIIDRRKGRDRRKM
                     MGNTGPDERREIRDRRQRRPGGSFPKLHGE"
     gene            complement(9299..10081)
                     /locus_tag="VF517_01865"
     CDS             complement(9299..10081)
                     /locus_tag="VF517_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051709.1"
                     /translation="MKKELRPVHEDVACDICGRTILKGERTEPYLAPGGQRMLVCDLC
                     TDRAYAEGWIRESAHGETPASLSRREPRRSILGRLRRRGPAAPAAPAVEAGPGSDGAS
                     DGLHDADGLPPLRDLEPEPEPYAEAAPPDAPPPPPPPSRPKDPRHVRAVPTNAQVKVE
                     RAVEVFNASEHPRTIAGIARSLGEPWVTATPLEASPSEVSIVVAWELSWYHYRVDLGT
                     EHDPVTLIGKGEELDELEPWAKEWNASASGDGSLAIGIRSQG"
     gene            complement(10138..12213)
                     /gene="fusA"
                     /locus_tag="VF517_01870"
     CDS             complement(10138..12213)
                     /gene="fusA"
                     /locus_tag="VF517_01870"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX8051710.1"
                     /translation="MPRQEADKIRNVALVGHRGSGKTSLNEAILFEAGAVNRLGSVAD
                     GTTVSDAAPDEKSRQMSISSSLASFTWQDRKINLIDTPGEPSFIADALAGLRVADGAV
                     FVVNAVMGVEVNTDRLWQRAAELGLARLIFINMLDRERADFFRSLESLKDAFGVHVVA
                     TEIPIGSEHEVRGVIDLVDMTAFEYEGAGRENCREIPIPDELREQAEQYRENLMDEVA
                     ENSDELMERYLEGEEIAHDEIVAALKKGVTEGHLFPVTCGVATQNLATNRLLDALVED
                     LPSPAMKGSIEAGNEVLEPDPSKEIVAYVFKTLADPFAGRINLFRVYQGTLTADTQVT
                     NCRAHVKERVGQLLVPQGKEMGQTDEFGPGDIGAVAKLKETHAGDFLAAVDHEVDLPR
                     PDLPAPVMAFAMEPKTRGDEDKVFTALRRLQEEDPTIDLHRDPQTGEQIVAGLSQIHV
                     EVIVERMRDRFGAEVTLKPPRVPYQETIKGRGKAQGRYKKQTGGRGQFGDCHIEIEPL
                     PHGEGFVFENAIKGGAIPHSFIPAVEKGIVETMQQGIVAGYPVKDVKVRLYDGSYHTV
                     DSSEMAFKMAGSMAFREAASQASPALLEPIMTVTVTVPEESVGDVIGDLSSRRGHPLG
                     MEPKGSMTAVKAEVPMAEMLTYAPDLRSITGGQGDYTMDFARYEEVPAHLAQKVIDAA
                     NEESEAVKA"
     gene            complement(12247..13182)
                     /locus_tag="VF517_01875"
     CDS             complement(12247..13182)
                     /locus_tag="VF517_01875"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8051711.1"
                     /translation="MEALREVAEVELAEPPRASLPARALWRERELPRLAARADALLAT
                     GPELPFRRLATPSIVVVHDLFPLTHPELTGRAKRLRFRAALPFVCSRATAIVCVSEAT
                     RASLEEHVAAARGKTTVMGEGPSELSPADPVAAERPYLLYAGELYRRKNLHTLLAALG
                     ALPDVDLVVVGRARAQTAAAFGRQVRDWGLTDRVRHEGFVPAERLAGLQAGALAVVLP
                     SLVEGFGRPLLDAMAAGTPALVSDIPALRELAGGAALLVPDPLDVRAWREAIERLCGE
                     PELRRELSERGRERAREFAWPRLAESMVELARTLG"
     gene            complement(13254..14039)
                     /gene="whiG"
                     /locus_tag="VF517_01880"
     CDS             complement(13254..14039)
                     /gene="whiG"
                     /locus_tag="VF517_01880"
                     /inference="COORDINATES: protein motif:HMM:NF004935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX8051712.1"
                     /translation="MAMQTNVKAVELKELWRKYKENGDERAREQLVLAFSPLVKYVAG
                     RMSTGLPAHVEEADLISYGLLGLISAIERFDPGREIKFETFAMTRIKGSIIDELRSLD
                     WVPRSVRAKAREIERANSKLEHQLHRAPTDQEVADELEMSMDDFQDALTRISNSSVIA
                     LDELWTLSDASGDQVSLLDTIQDPDAVDPAHAMDLTETKDRLAESIARLPEREKLVVA
                     LYYYENLTLREIGEVLGVTESRVSQLHTKAVLRLKSALQADSD"
     gene            14225..17512
                     /locus_tag="VF517_01885"
     CDS             14225..17512
                     /locus_tag="VF517_01885"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8051713.1"
                     /translation="MAPTPLTHTLGGGRGGILTLASALCLALLLAIAAPASAVTHKRA
                     AKKAVAALGSSHASGAVVVLGLPKPLRAGTLVTQQGKKKVVARVGRERAFFYYEDAAP
                     LQPFPHAGRVALVGVKSGKVRLTRALTRRPLLNGRLPAFLKSVRAYKNEGYVVYDSST
                     KLSAPAPQAPPVGSETTTQQDPFPIEPVGVNRRPTAFDQNVAAKLNSPKHIILTGSDP
                     DTAPGGDSEGTLLTFAVTKLPNHGSLSGQPPSLTYTPDPGWSGPDHFFFKVNDGDLDS
                     KEGKVSINVVALGAPPVVTTSTGCTEYLEQTAGVAIDGQLTASDPDDTVLDSAVVRIA
                     SGLEGGDNLLFTDQNGITGAYDERTGQLDLVGTASVATYQAALRTVRYQNLSSGTPAA
                     SKGIAFTVNDAGSDSAPATKEVCITEAGGPNNRPVGETSEGVLDYIENDGAVSVDPGF
                     FVLDPDSSQLSGATVRFTVSQPPEDDNGNPIGSPVNNFAPDEDDLAFTDQNGITGAYD
                     DSTGVLTLSGLASVADYEAAMRSVTYENSSEDPSPAPRTLRFQVKDASGLNSVASNRG
                     VLVTPVNDAPTATPSEGASSYTEDEPASAVDSQLDIGDVDNDQLQGAQVKIASGLQSG
                     DELAFEDQLGITGSYDAETTVLTLTGTASVDDYETALRSVTFRHVGDDPDPARSVEIV
                     VNDGELDSATATKEIAVTPVNDAPVATTGAGSTAYTENDPATTIDTAVALSDVDDDQL
                     EGAQVKIASGFQPGDELAFVDQLGITGEYDTTTGVLTLTGTAPIGDYETALRSVGFSS
                     FDDDPVASKTVEFTVNDGDDDSAAASREIAVTPVNDPPVVSTSDGSTEYEIGGPGVAV
                     DPQLTVSDPDDTSIASAEVTISGFEAGDELVFVDQLGITGTYDTETGVLSLTGTASVG
                     DYEAALRSIEFGTTNETPSASRSIGFTVNDGDAGSAAASRAIDLVEANDAPVVTTTTG
                     DSTYTLGDSAGVVVDDLLTVTDGDDTNLESAVVRIQGGEPGDELVFTDQAGITGTIDE
                     TGFLILTGTASAADYETALRSVRFRNTTATEAGTRTLEFQVNDGRDDSATATKGVALV
                     AAPPPAP"
     gene            complement(17565..18491)
                     /locus_tag="VF517_01890"
     CDS             complement(17565..18491)
                     /locus_tag="VF517_01890"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX8051714.1"
                     /translation="MAPSAPRDYEPTPRWSSALAAFDGSMRARGMAEKTRRAYGVDLG
                     QFAEWASAQGHEPDAMDHRTLRRFAGVLSERGNSRSTVARKLAAIRAFYRHLVERGEL
                     AGSPADLVSSPKRDSYLPRVLRADEVAAILDAMPASEPLELRDRAMFELTYSAGLRAE
                     EVVNLDAGDLDPDGEELRVTGKGDRTRVIPAGEPAWRAVERYLARARPRLANRADPDP
                     ALFLSKSGRRLSTSDVRRRLAVATRRAAAGAGVSPHTLRHSFATHLLEGGADLRTIQE
                     LLGHATLSTTQTYTRVESKRLRRVYAETHPRA"
     gene            complement(18503..19327)
                     /locus_tag="VF517_01895"
     CDS             complement(18503..19327)
                     /locus_tag="VF517_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEX8051715.1"
                     /translation="MNAGPAPGDCNDGNAAIKPGATDVPDNGVDEDCSGADAKSAPLD
                     SDGDGVPDAQDQCKTEKGTLANGCNPAPGPGPAPPDSDGDGVPDSSDPEPNDPTIPTK
                     FGADNGNNTVDGTAAGEIICALLGNDVVHAQGGNDTVFGDLCNVKARIASAQAGAGGS
                     DTLFGGTGNDTLYGAGGNDKLFGEDGADKLFGGGGDDSLNGGKGKDSLDGGKGNDKLT
                     GSGDVNKYAGGSGDDSINARNGKKETVDCGAGKKDSASVDRADKVKGCEKVKRAKK"
     gene            complement(19325..19489)
                     /locus_tag="VF517_01900"
                     /pseudo
     CDS             complement(19325..19489)
                     /locus_tag="VF517_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(19529..19864)
                     /locus_tag="VF517_01905"
                     /pseudo
     CDS             complement(19529..19864)
                     /locus_tag="VF517_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(19859..20407)
                     /locus_tag="VF517_01910"
                     /pseudo
     CDS             complement(19859..20407)
                     /locus_tag="VF517_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            20483..20767
                     /locus_tag="VF517_01915"
     CDS             20483..20767
                     /locus_tag="VF517_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051716.1"
                     /translation="MGSFFIDVQDGRVATRTQLEAAGVVSADVAGPPPRPWHPVQGPG
                     DASMMWYAVMRKRLRGVFIGTLCLRHTGRQAALEAEGWEEIPVERIGLGA"
     gene            20764..21477
                     /locus_tag="VF517_01920"
     CDS             20764..21477
                     /locus_tag="VF517_01920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8051717.1"
                     /translation="MTEAPGRRDRSWRLPFLDDPDWQMWAGERAALEGVLSALRPRLS
                     IEIGTAQGGSLRRIAAHSGAVHAFDVDPAVAEVVRAVPNAIAHVGDSAATLPPVLAEL
                     AAAGHNVDFALVDGDHSAAGVQRDVRALLASPACSRTVVILHDSANEAVRDGLEALGL
                     PDHPKVRVWMPDFVPGYLVGARHERAGEIWEGLALAVLDVDAPPGPAVQDEVHENVSA
                     VYRAFRDAKARAAGSPARP"
     gene            complement(21566..22474)
                     /locus_tag="VF517_01925"
     CDS             complement(21566..22474)
                     /locus_tag="VF517_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8051718.1"
                     /translation="MLVLIALALPASASAQATRTWVSGVGDDANPCSRTAPCKTFAGA
                     ISKTAAKGEINCLDPGGFGGVTITKSLAIKCHYTEGGVLVSGTNAIVVNAAATDNVTL
                     RGLDINGIGTGLNGVRVLQAKSVKVLDSEIFGFVRNAIDFEPANNTARLVVSNVDVHH
                     NLGNGVFVHPQSIAGGAKATVRKSDFDSNGCGLTVSSQGFDAGFNYAVNCGTAGPGAF
                     NVGAQLSGFRNSITDSNGVGVFVRGSNALQRMSQTEVTGNQVGLQQLDSGQLKSWGDN
                     RVDGNVTNATAGTPTGPNLTFTKRKR"
     gene            22569..23483
                     /locus_tag="VF517_01930"
     CDS             22569..23483
                     /locus_tag="VF517_01930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8051719.1"
                     /translation="MDQNGTKPTLEIIIVTSNGAKQHIRACLRSLELNPLTSGSMLVH
                     VVDNASPDGTPAVVREEFPWVKLTALDWNAGFCIGNNVGMREASAPYVLLLNPDTEVY
                     EGALDHMIRVMEERPDVGMSSCRLAQPDGTFDHAAKRSFPSPLGALAEFVGVGRGGRM
                     PGTLEGVADYRAHDIGEYEEGEVDAVNGAFMLCRREAIEAVGLLDEQYWLYMEDLDWC
                     YRFKQAGWKVWYDGRVTVMHVKGGTTKVKRHRGLRHNTAFHRGMGRFYRKFYAGERPV
                     LDWAVYLAIGVKLAVSVARSTVARRRLT"
     gene            complement(23476..24468)
                     /locus_tag="VF517_01935"
     CDS             complement(23476..24468)
                     /locus_tag="VF517_01935"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051720.1"
                     /translation="MARPRAAGAHGRSRRRLPGLDRVAELSRRVLLTGAAGFIGRPAL
                     AALLDGGADVHAVGRGPAPDWALRPAEWHRRDLLAPGAAAALVAEVAPEAVLHLAWET
                     TPGAYWTSPANEAWLRASLELAEAAAARGARVVTAGTCAEYDWSAGWLSEAGTPLAPA
                     TLYGRTKQALGSSLEELGRASGASVAHGRVFFLYGPHEAPARLVPSVTTALLRGERAP
                     TTDGVQRRDFMHVDDVAAALVALLDADAVTGPVNIASANPVAVRDVVAAIADEVGRPD
                     LVGWGEVERGASEPDLVAGDAARLRDEVGFSPRYGLRDGIRATVGWWRGRMGVR"
     gene            complement(24390..24935)
                     /locus_tag="VF517_01940"
     CDS             complement(24390..24935)
                     /locus_tag="VF517_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX8051721.1"
                     /translation="MTFEPSAVSGAVVVVPEPVEDERGLFARTVDRDLFAEHGLPTEW
                     VQCSASYTRHAGTLRGMHLQLAPHEEAKLVRCVRGAVYDVALDLRPDSPSFRAWAGVE
                     LSEANRRALHVPPGCAHGFLTLTDDVELLYMMDAPHVPGAARGVRWDDPAFAIEWPGR
                     VRPELMADRDAGYPDWTGSPS"
     gene            complement(24932..26197)
                     /locus_tag="VF517_01945"
     CDS             complement(24932..26197)
                     /locus_tag="VF517_01945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8051722.1"
                     /translation="MTTEGVSIAGVGSTTCRFCGAVARTSFADLGMSPIANDYLSPEQ
                     LNGMEPFYPLRALVCPDCFLVQLQDFETPEAIFADDYAYFSSYSTSWLDHCQRYVDYA
                     IERFRIGSGSTVVEVASNDGYLLQFFARRGVRVLGVEPAASVAQVAQVKGVPTVQRFF
                     GVNTARDLLPDAQADLLIGNNVLAHVPDLNDFVAGMKVLLAPQGVITMEFPHLQQLVD
                     HVQFDTIYHEHFSYFSFLTVRRVFAAHGLELFDVDELPTHGGSLRIYARHAESDHEPV
                     TRRVTELVEREREAGYEDLEVYRTFAERVKATKREVLRFFVDAKEQGASIAGYGAPAK
                     GNTLLNYCGIGPDMLDYTVDLSPHKQGKFLPGTRIPIHAPEEIARTRPDFVFILPWNL
                     RDEIVEQMAHVREWGGRFVVRTPSFEVIE"
     gene            26278..27288
                     /locus_tag="VF517_01950"
     CDS             26278..27288
                     /locus_tag="VF517_01950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8051723.1"
                     /translation="MRGALTATGPAVSAVVLTYSRQDLLPDCLSSLATALAEVPEPTE
                     IVVVDNGSPGNVATGRVREWCPDAKIVVLTENRGYAGGIVEGIRRARGEWILTVGDDS
                     TVDAGAVVALLAAGRSAPDVGSVAAKMLFAEAAGGAVINSAGLEIDRLGIAADRLVGQ
                     PADAGERDVTEVFGTSGGGALYRAAMLDAIGSFDASFGLYLEDADVAWRARRAGWRCL
                     YVPEAIVYHHHSATTGHRSPGKYRYVGRNRVRLVAKNATRRQLLRYALPMIGYDLAYV
                     AYAALANRTLAPLHGRLEGLRHWRADRRAARGEVEVELAPVLGLRAALRRNRAWRLGS
                     GG"
     gene            27285..28709
                     /locus_tag="VF517_01955"
     CDS             27285..28709
                     /locus_tag="VF517_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051724.1"
                     /translation="MSARETPPPRLSVPRSAGVFLAATTISHLSQFVWLAAGSRAMSH
                     DDFGAVLAAQVLYAVLQIAIDNGTAQLGAREAARGAMTDLDLGRLVRLRLALTAVAVP
                     AGLAIGAASAGLAATAPFMAALLLFALVNVWRPYGQGDPRPWAGYMFGRSVLPAAVAS
                     AYALSGGSFPVELAGGLECVVLVVLIAATGQRPLRELRLALRSRGMPWRPVLSIGVPA
                     MLQQASVAAGTIGLSAGGAPRAAGALAACVRLLTGLNALNGIVATAMFPSLAERGGLG
                     AGVRRVVLGLRMTTAVAFAATAAVCLVAKPLAQTLLDRTSDAIESALVLTTAGAAGMG
                     TIVMLTYLLVAASGERRVLPAYAAGAGVTLAGVTLVVASGGERVDTIAALLVAGQLTT
                     AAGLVLPVARSLPDYRGPARAALAGAVALAAVAVTAVVTDAAVPAGVVLAAVAVAVGW
                     GGARPLVGVLRTRGAGPRPTAGSR"
     gene            28706..>29601
                     /locus_tag="VF517_01960"
     CDS             28706..>29601
                     /locus_tag="VF517_01960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8051725.1"
                     /translation="MIDAVILTSNSRDMALRCVAHTKADPTVDRVFLVDNASSDDTVA
                     AVRAEHPEAEVIALAEHTGLAAALNRGAEAGSNPYVLYLNDDVFAAPGAVATLLAALE
                     GRPGAVAAGGRLVNEDLTTQDQYRPRDFPGPVTISLRLLGLDRLWPRNPFTGRHLRHP
                     LDDETTVEADQPAGACLLVRREAVEAVGGWDARYWFWYEDVDFSRRLADTGGATLYVP
                     SAPFRHLGGASTTRWSLPEMHRRTYYGMLLYAETHFARAGRLLVAATMAVVCLLRLAA
                     TRGGAGAGGYSGCLVQARARAAG"
BASE COUNT         3917 a        10589 c        10681 g         4414 t
ORIGIN      
        1 tgatccggca ggagatggtc gacgcaccgc gcccgtgcga tgcggtgcgg aggtgggccg
       61 ggaacggctg gtcgccgtcg gcctcgcccg gagatccgcg gcagggcctc gcggccgcgc
      121 aggcggccga gcgcagcctg ctgcgcgtcg gccggaccct ctcggcactc ggcgtctcgc
      181 cacgcgccgc ccaggggatg acaccgggcg ggctgatcga gctggccgcg ccgacgatca
      241 tcacgtgccg cagcaggcgt gagctgacgg cagcgcaggg ctgctcctag taccgagggc
      301 ttggagcggg cggcggtccc cgggatcgcc gcccgtctcc cccttccagt ttccagccac
      361 ccgactgggg gtaactcccc acgcagagag gcagaagagt gcacggggcg cacatcaggc
      421 gggcattccc gccgcacgtc ctcgcgatgg tgttgctcgc ggggcttgtc gtcgcagggg
      481 tggcggtggc ggccgagtcg ctcgaccgtc cgttcggttt cttcagccgc gagccgtcgg
      541 aggtgctggg cgctccgttc tacacgggct tcctcttcca cgtcgggctg ctcgtgtggt
      601 tcgcgagcgc cgtcgcgtgc ctgttcggcg gctgccttgt cgccgcgctg cgcggacgcc
      661 gggcggccgc tcctctgctt gccgccgggc tgctcagcac ggtgctcgcc ctcgatgacg
      721 cgctgcgcat ccacgaggac ctccttccgg gcaagctcgg gttgccgaag atcgggacat
      781 acgccctgta cgccgtcgcc ttcggcgcat ggctctggtt cttccgcgac ttcgtccgcc
      841 gcaccccctg gccactcctc ggcttcgcgg gcctgatgct cggtacttcg gttggcctcg
      901 accgcgtctt cgagtcccat cagcgccacc tgttcgagga cggggcgaag ttcgtgggga
      961 tcgtggcctg gaccgcgtac ttcgtcagga cgtcgttcgc gagcgcgctc gaggcggtgg
     1021 cggcgcgacc ggtgagcgac gagtcgaagg cagcgctcgc ggagccggct gcggcgcccg
     1081 cgcgaggcga acggcggttc gagcgccggc gggaccgcca gccgctcccg cgctagggcc
     1141 ctcgctagcg ccgcttgctg cggcgcgcct ggacggtgat caggcgcttg acgagccggg
     1201 ctgcgagcgt ctgcccggag atcgcgtcga gacgagcgat ggccttgccg attgcgcgcc
     1261 ggcgctgccg gatctgctgc accatccgcc gcggcggctg cacgcgaacc gtctggctgc
     1321 tgccggccgc cacgtcgaac cgcgccgacg acgacgccgc tccgctgacg cgcctggtcg
     1381 ccgacgtggc gatcaggttc acgcgccccg tgcagcgcgc acccgcgccg ccgcggcaga
     1441 tcagcttcag cttgatacgc ccgcccgcgt agggaacgcg ccggtgcgag atcacgagct
     1501 tggcctcggg cgcggtgacc gggccgggtc cggggccggg atcagggccg ggtccgggtc
     1561 cgggaccagg tccgggaccg ggatcgacgg gcgggtccac gaacgcgacg tccgtcgtcg
     1621 ccgactggtt gtcgagcgtg gacgtgaccg tgctcgtgcc cgtctgtgtg cccgaggtga
     1681 gcgtgccggt cgcctggccg tcagcgatgg tggtggcggt aggcgcgatc gagccgagcg
     1741 tggccgcaaa ggtcgacggc accgccggga agaacggcga cggctgaacc gcaccggccg
     1801 agttgcggcg cacgctggcg acgatcgacg aggtcttgcc gccggtctcg atcgtggccg
     1861 ggctggcgct cgcgccgagc acgagccgcg gatcgctgtc gacggtgccc gtggtcgggt
     1921 cgcagcccgc cgcccccggg ccggcgttgc agccccacca gttgttctcg gcacccacgg
     1981 agcccggcgt ggtgttgttg acgccggcgg tgttgccgag gatccggttg cgtgcggcga
     2041 cgatcgcggg cgaggccgag ctcgagctgt tgcggaagag gtcgatgccg gcgctcgtcg
     2101 agccgatgtg gttggccacg agggtgagcg tcgggaggaa ctggttccca ccctgaagga
     2161 acgctccggc gcgcacgccg gcgttgccgg gaccgaggat gacgttgctc gtcagcgtgg
     2221 cgacgccggc ggtggcgtac acgccggcga accggttgcc cgcgatcgtg ttgccgctga
     2281 tgctcgacgt gcccgcgcga gtgaggacgc tggaccgcgg cctgatcccg ttgtcgttgt
     2341 tcgacacgac gttgccggtg aggtcgacgt tcgagtcgat cagggcgatg cccgacgact
     2401 gcgactgggt ctgcgggtcc gggccgcacg aggcctggtt gcagaggtgg ttcttcacct
     2461 cactcgcgct gatcgtgccg gtggggaccg ggccggtcgg ctgtgtgccc ccggggctgc
     2521 cctgcggcac gatcgcgctg ctcacgacga tgccgttctg cgcgatgtcg gtctgcgcgc
     2581 ccgcgccggt cacgtccacg ttcgagagcg acatggcgag gttcgtgctg ctgatgctca
     2641 ccccgttctt ctggaagtcc gagaccgcga tgtcggacag cgtgagcgtg cgtgccggcg
     2701 gcgccgcgtc gtcgttgaag acgtagacgc cgttgccgac ctggcagccg tcgagcgggg
     2761 tgtggcggaa gccggtgatg gcgagatcgt cggcggtccc gcccgcccgg cggaacgcga
     2821 ggccatggaa gttgagcgtg caggtgggcc cgccgcgccc ggcgccgtcg atcgtcatgt
     2881 cctcgaccgt cgcgccggcc acgtcgtgca cccagaccac cggcgcgttg tcgggcgcga
     2941 gcgtgtcggt ggtgaacttg ctgaccgggc cggcgggcga gcggattatc gtctgcccga
     3001 cgcctgctcc ctggaggacg aggccgttct tcgcgacgtc gacctgctcg gtgtaggtgc
     3061 ccgccccgac cgtcaccgtc tcgccggccg cggcgaggtc gacgccaccc tgcacagtgg
     3121 tcgcgtcgca gccggcggcg cagacggtct tgacggcgct cgcctcgctc gcgaagagca
     3181 ggggcaggac caggacggcg ggcaggagca gcttccgcat cggcagcacg ctaccccagg
     3241 ccggggacac cactttgaag taaccgcggc tgcgcagctt ttagcgcgcg aggccgccga
     3301 ttagctcaca gcgcgctccc gtcctggcgc actcgctgga tgaccatgcg gaagttccag
     3361 ctcagcttgc ccgtcatcgt cgagattccc tgctggaccg agtaggtctg gctgttggag
     3421 gtctcgacca cgaacggctt gctgcccgtg aagtcgccca gcggatgggt cgtctccagg
     3481 aggtcgcggt tcacgtacag gtccggcacc tggaggctgc acgcgctgtt cccgagctgc
     3541 accgcgacga acgaccagct cagcttcacg cgcccgtcgc cggtgttgcg caggttgatc
     3601 ccgaggttct ggttcgggat cgactcgggc gcgtagttgc acggcgtctg cgaggtcggg
     3661 caggtgtgga gcgtggagta ggcgccccgc ctgtaggtga tcggcgtcca gatcgtgccg
     3721 aactgctcct cgagctttcc gtgcgggacc gtgcccggct gggccggtgg gctgatctcg
     3781 acgtgctgag ggccgcccgt gaaggtgcac tggccgctgg tggtcttgac gtcggtcgcg
     3841 tcgaccccgc cgctcacctc gagcaccttg aagtagaccg tgccctcgat cggcttgaag
     3901 ctcgcgaccg cctcggccac gccggccccc gacttcagga tgagccgcac gccggcgggg
     3961 ttcgacgccg gccacgtctg cggcggggcg gtgaactcga tccagtcctc ccccgccttc
     4021 cagggcgtgg cgccggcgag cgagcggtag ctctcaccct gggtcgtcag ctcctgggcc
     4081 aacacgggcg agttggcgat caggtggtcg acggtcgccg atccctgctt gaggcgcggg
     4141 accccgtgca ggttgacctt cccgcccgga gcgaggaact cgggggcggg gatcgtcatg
     4201 tcgacgcacg tgttcggcgt ctggaagtgc cgctcggcgc ctgcgaagtc gcgctcgatg
     4261 gcgaggtaga cgacgctcac gaagcgctgc gcgaggtcgg accagacgcc gctgcgcagg
     4321 ggccggcggg gggccggcga ctcgtcgcgg tcctgctgct cgacgaactc gtcgaagcca
     4381 ccgtcgagga tcagcagcgg cgacagcgac tcgctgaagc gctgggtcgg cccggtcttg
     4441 cgggtcttgc cggtgcggcg gtcgcgggcg gtctggctgc gcgtgatcga gaccttcccg
     4501 gcctcggcga agatgcccac catgctggcg ccgccggtct gcgagtggac ctcgaactgg
     4561 acgtcgagga tcacgctggt cgtcacgtcg tagcggccga cgcgctcgac cgtcgtgacg
     4621 acgtgctccc cgcgcgccct gcccttgatc tcccagtagc cgtccgcgtc cacgcccggg
     4681 ttgtgggtcg ggcagaggga gtcgagagcg gtctcgatcc tcagccggcg gtgctgcttg
     4741 gcgttcttgc ccttgatcga gtcggcggca ccgagggggt cgccgccgaa gcccgcggcc
     4801 gacttgcgca gcccgccggc cgcgggggcg gcgatggcgg ccgcgaacgc gctgtcggcc
     4861 ttcctgacct tcgactccca cctgctgccg agcagggcct tgtagtgcgg tagcccgagg
     4921 cccgtccggg ccttctggcc gcgcgttgcc tcgaggagtc cgcgcacggc gtcggccggg
     4981 gcgggcgcct ccgcacgcgg cgcctggccg gcgatcggct tggtggccgc attcgcggcg
     5041 ggcgcgacgg cggcgacgag gaggatcagg gcaagcggga tgagtcgatg cgagttcatg
     5101 ccgccacgct aagcgccgcc gggagccccc acatcggggc cgatccccaa ggatcccccc
     5161 aatctttggg cagccccccg ggcccaaggg attgggggtc gatcggtttg gagcgctcag
     5221 gacctcgtgt cgcgggtgtg attcactcac cctgtggcca ggctggggag caaggcgcgc
     5281 gcgctgctga tcaccgttct gatggcagcc gcggtcggcg gtggctgggc catcggggcg
     5341 agcagcgaca tggacgagat ggagtcgcac gacacggggg ctccggcgtc ggcggcggag
     5401 gacgatcgcg acgtggagcg cggcttcctc caggcgatgg tcccccacca cgagtcgggg
     5461 atcgagatgg cccagatggc cctcgagcac tcccaggcgc cggtgatcag gcggttgagt
     5521 caggcgatcg tcgacaccca ggcccgcgag atagagcaga tgcggaagat gcacgaacgc
     5581 ctcttcggcg agccgctcga gccggacggc gacgcccaca tggccctcgg gctgtcagcg
     5641 gaggaggccg gtatggccgg catgagccac ggcggggaga tgatgcggaa gctggcggat
     5701 gcgaagccgt tcgaccgcgc ctacgtggac gacatggtcc cgcaccacca gggcgcggtc
     5761 gcgatggccg aggtgctgct ggagcgcacc gacgacgccg agctgaagcg gctcgcccag
     5821 gcgatcatcg acgcacagcg gcgtgagatc gcgacgatga acgacttccg gaccaaggag
     5881 tacggcggcc cagtgcccga gaagccgggc gatggcgggc acggcgggat gaagatggag
     5941 tgacgggcgg cggcctccgg ccgcctacgg cgtcgtgcag gagcccggca cctcgacgtt
     6001 gcggtcgatg cactcgcgca ccggccgggc cgcgttgctc acatccgtga gagcctcgtc
     6061 ctcgtccgga ggcgccggaa cgggatcggt cccggtcggt ggcggcagga tgtcgccgcc
     6121 gcagttttac cgtcggttca atgcgcggcc gccgccgagg tcggcatcca cggctcgtcc
     6181 acgtgcgcga gccagaccag ctcgggctgg agcgcatgca ccttcagctg gccctcggac
     6241 tgcggctcgt cgagctcacc gagccggatc gccacgtcgc cgccgacgat gaccggacgt
     6301 tcgccggtct cgatcacgac catctgcatg ccgcgggtgt ggcccggcgc cgggacgagc
     6361 ctgagcccgg gaagcagctc aagctcgccg tcgaccggct cgtaccgcac tccgggcgcc
     6421 tcgacccact cgcgaatggt gtagtcgtcc tggctgagcg cgtcgtccag ctcctgacgc
     6481 tggacgtaga tcggcctgcc ggcgaagagg tggttgccgc cgcagtggtc gaagtgcagg
     6541 tgcgtgctga cgaccagatc gatgctgccg agatcgaggt cctgctcgcc gagcggctgg
     6601 aggcggggat ccatgtcggc caccgcgggg tggagctccg tcatgccggt gtcgaccagc
     6661 acgcgaccat cgggatggtc gatgacgtgc acgttgacgg gcatccgctc gtcctcggcg
     6721 agcaggtcgg ccacgcggat cggcgtgacg gtgactgggg cgggagcgct catctggtga
     6781 tcctctcgtg tcgtttcgaa ccggcgaccg ccgcgagcac gagcacggcg ctcgcggcag
     6841 tgtgcccgac cgctggacac gtgggaagat ccaggaatgg caccaaaggt gagaccagtt
     6901 ggacggcagg aggcgagccg gccgatcgac ctcgtcatcg ccatcgaccg cgatgcgccg
     6961 gcgacgctcg gcgcgcagat cgagtcgcac ttccggcgcg cggttcgcga cgggcagctg
     7021 cgggccggca cgcggatccc gtcgacgcgc gacctcgccc gccagctctc ggtgtctcgg
     7081 cgcgtgaccg tggacgcgta cgcgcaactc gccgccgagg gctacctcgt ccttcgccag
     7141 ggggcgcacc cgcaggtcgc cgacggcgcg atcgcgaccg atcgggagcg gcgcgacggc
     7201 ccggagctgg cgcccgaggt gctcccccgg ttcgacttcc ggcccagccg cccggacgtc
     7261 tcggcgttcc cgcggcaggc ctggagccgg tcgctgagga ccgccgtggc ctcgatcgcc
     7321 gacgccgagc tgatctacgg caacccgtgc ggcgtcgatc cgctgcgcac gacgctctcc
     7381 gagtacctcg gacgggtccg cggcgtcgtc ggcgctccgg ggaacgtcgt cgtcacgagc
     7441 ggctacctcc agggtctcgg ccttgcctgc cgtgcgcttg ccgccggcgg cgcgcggcgc
     7501 atcgcgctcg aggaccccag cgcgccggag cagggcccga tcgcacgccg ggccggcctc
     7561 gagccggtac ccgtgcccgt cgacgcgtcc ggcctgcgcg tcgacgcact cgatgcggcc
     7621 ggtgtggacg cggtcgtcgt cacgcccgcg caccagcatc cgaccggcgt ggtgctcgcg
     7681 cccgagcgcc gcaccgccct ggtcgactgg cttcggcgca ccgacgcgtt cgccatcgag
     7741 gacgactacg acgccgagta ccggtacgac cgtgccgcgg tcaaggcgtt ccagggcctc
     7801 gatcccggcc gcgtggtcta cgcgggctcg acaagcaaga cgctcgcgcc ggcgctgcgc
     7861 atgggctggc tcgtcgtccc ggggcgcctc ttggacgcgg tcgccgagga gaagttcctc
     7921 gccgaccagg gcagcgctcg catcgagcag cacgccctcg ccgacttcat cgcgcgcggc
     7981 gagctcgacc ggcacctgcg ccggatgcgc cttcgctatc gcgcgcggcg tgacctcctc
     8041 atcgacgccc tcgccgacca gcttcccgat gcacaggtcc agggcatcgc ggcgggtctg
     8101 cacgtgaccg tccggctccg cggcaccttc gacgaactgg ctttgcgcga agccgcacgc
     8161 gcgcggcgcg tcgagctcag cacgctcagc gactacaccc ccggagcgtt ctcccgcgac
     8221 cccgtactgc tcgtcggcta cgcgcaggtc tcggaggccg ccatggccgc gggcgtacgc
     8281 gagctcgccg cggtcgtcgc atcgtctcga accgggtgac tcagttcagg gcaggaagca
     8341 gaggcgccga ccggaatcga accggtgtga acggctttgc aggccgctgc gtaaccactc
     8401 cgccacggcg ccggtgtggc gcgcggaagg ctagcggcgg ggccggaggc cagcgcgtcc
     8461 ccagaacgcc gagagcccgc cgcagcgggc cctcgatagg agcggatgat gggattcgaa
     8521 cccacgacct tcgccatggc aaggcgacgc tctagccagc tgagctacat ccgcacggcg
     8581 cggcgagtat agacctcgac cccggcgccg tcgggcttga atggtgcgcg tgcgaagccg
     8641 cccggggcgg agcgccgcgc ctcagcggcc ggcccggtcg agctcctcgt tcacgagctc
     8701 gtccgcgcgc ttgttgtgct cgcggcgcac gtcgcgcacc gaccacttgt cgaactcgcg
     8761 cagggcgcgc atcgtctcca ggaacagcgg cttcagcccg gcgtgcttga ccttgtactg
     8821 accgccgatc tggcgggcta tgagctgcga gtcgttcacg acctcgaccg ccgaagctcc
     8881 cagctcccgc gcgagctcga ggccgagctg cacggccttg tactcggcca cgttgttggt
     8941 cgtctcgccg atgtagcggc tgcgctccag gagcgggtcg ccgtccgggc tggaggcaac
     9001 ggcggcgacg gccgacggcc cggggttgcc gcgggagccg ccgtccacgt ggaccacgac
     9061 cgactcgatg gccaccccta ctcgccgtgc agcttcggaa acgagccgcc ggggcggcgc
     9121 tggcgacggt cgcggatctc gcggcgctcg tcgggccccg tgttgcccat catcttgcgg
     9181 cggtcgcgac ccttccggcg gtcgatgatg acctccacgt cggggtcgtc cttgtagtgc
     9241 tcgaccatcc ggtcgtagag ctccgcctcg agcgcctcgg ggatcacgca gtagatcgtc
     9301 atccctgcga gcgtatcccg atcgcgagcg aaccgtcgcc cgaggcggac gcgttccact
     9361 ccttcgccca gggctcgagc tcgtcgagct cctccccctt gccgatcagc gtgacgggat
     9421 cgtgctcggt gccgaggtcc acgcggtagt ggtaccagga cagctcccag gcgacgacga
     9481 tcgacacctc gctcggcgag gcctcgagcg gcgtggccgt gacccacggc tcgcccaggc
     9541 tgcgcgcgat cccggcgatg gtccgcgggt gctcggaggc gttgaacacc tcgacggccc
     9601 gctcgacctt cacctgcgcg ttcgtcggga cggcgcgcac gtggcgcggg tccttcggac
     9661 gcgagggcgg cggcggaggg ggcggcgcat ctggaggagc ggcctcggcg tatggctccg
     9721 gctccggctc gaggtcgcgc aggggcggca ggccgtcggc gtcgtgcagg ccatccgagg
     9781 cgccatcgga ccccgggccg gcctcgacgg cgggcgctgc cggcgcggcc ggcccgcgcc
     9841 gccgcaggcg cccgaggatc gaacgccgcg gctcccggcg gctgagcgac gccggcgtct
     9901 ccccgtgggc cgactcgcgg atccacccct cggcgtaggc acgatcggtg cacaggtcgc
     9961 agaccagcat ccgctggcca ccgggggcga ggtacggctc ggtcctctcc cccttcagga
    10021 tcgtccgccc gcagatgtcg caggcgacgt cctcgtggac gggtcgaagc tccttcttca
    10081 tgggccgccg ccgatgctaa cgcgagcccg cgggcgcgcg ggcgccgtct tgcagggtca
    10141 ggccttgacc gcctcggact cctcgttcgc cgcgtcgatc accttttgtg cgaggtgcgc
    10201 tggaacctcc tcgtagcggg cgaagtccat cgtgtagtcg ccctgcccgc cggtgatcga
    10261 gcgcaggtcc ggcgcgtagg tcagcatctc cgccatcggc acctcggcct tcacggccgt
    10321 catcgacccc ttcggctcca tcccgagcgg gtggccgcgg cgcgagctga ggtcgccgat
    10381 gacatcgccg acggactcct ccggaaccgt gacggtcacc gtcatgatcg gctcgagcag
    10441 agccgggctc gcctgcgacg cggcctcgcg gaacgccatc gagccggcca tcttgaaggc
    10501 catctccgac gagtcgaccg tgtggtagga gccgtcgtag agcctgacct tcacatcctt
    10561 gaccgggtag ccggcgacga tcccctgttg catcgtctcg acgatcccct tctcgaccgc
    10621 cgggatgaag ctgtgcggga tcgcgccgcc cttgatggcg ttctcgaaca cgaacccctc
    10681 gccgtgcggc agcggctcga tctcgatgtg gcagtcgccg aactggccgc ggccgccggt
    10741 ctgcttcttg tagcgcccct gtgccttgcc cctgcccttg atggtctcct ggtagggcac
    10801 ccgcggcggc ttgagggtca cctccgcgcc gaagcggtcg cgcatccgct cgacgatcac
    10861 ctcgacgtgg atctgcgaga gcccggcgac gatctgctcg ccggtctgcg gatcgcggtg
    10921 caggtcgatc gtcgggtcct cctcctggag ccggcgcagc gcggtgaaga ccttgtcctc
    10981 gtccccgcgc gtcttcggct ccatcgcgaa cgccatgacc ggagccggga ggtccggccg
    11041 cggcaggtcg acctcgtggt ccaccgccgc gaggaagtcg cccgcgtggg tctccttcag
    11101 cttcgccacc gcgccgatgt cgccgggacc gaactcgtcg gtctggccca tctccttgcc
    11161 ctgcgggact agtagctggc cgacgcgctc cttcacgtgc gcgcggcagt tggtgacctg
    11221 cgtgtccgcg gtcagcgtgc cctggtagac gcggaacagg ttgatccgcc cggcgaacgg
    11281 gtcggcgagc gtcttgaaga cgtacgcgac gatctccttg gacgggtcgg gctcgagcac
    11341 ctcgttgccg gcctcgatgc tgcccttcat cgcgggcgag ggcaggtcct cgaccagcgc
    11401 gtcgagcagg cggttggtgg cgaggttctg cgtcgccaca ccgcacgtga ccgggaacag
    11461 gtggccctcg gtgacgccct tcttgagcgc cgccacgatc tcgtcgtgcg cgatctcctc
    11521 gccctcgagg tagcgctcca tcagctcgtc ggagttctcc gccacctcgt ccatcaggtt
    11581 ctcgcggtac tgctcggcct gctcgcgcag ctcgtcgggg atcgggatct cgcggcagtt
    11641 ctcgcgcccc gcgccctcgt actcgaaggc ggtcatgtcg accaggtcga tcaccccgcg
    11701 cacctcgtgc tcggagccga tcgggatctc ggtggccacg acgtgcacgc cgaaggcgtc
    11761 cttcagcgac tcgaggctgc ggaagaagtc cgcgcgctcg cggtcgagca tgttgatgaa
    11821 gatcaggcgc gcgaggccga gctcggcggc gcgctgccag aggcggtcgg tgttgacctc
    11881 gacgcccatc acggcattga cgacgaacac cgcgccgtcg gcgacgcgca ggccggccag
    11941 cgcgtcggcg atgaagctcg gctcgccggg cgtgtcgatc aggttgatct tgcggtcctg
    12001 ccaggtgaag gaggccaggc tcgaggagat cgacatctgc cgcgacttct cgtcgggcgc
    12061 cgcgtccgag accgtggttc cgtcagccac cgagccgagc cggttgaccg ccccggcctc
    12121 gaagaggatc gcctcgttca gcgacgtctt cccgctcccc cggtggccga ccaaggccac
    12181 gttgcggatc ttgtcggcct cctgtctggg catgtctctc tccccttcct cgctgaaggg
    12241 cgcaagttac ccgagcgtgc gcgccaactc caccatggat tcggcgagcc gcggccaggc
    12301 gaactcgcgc gcccgttcgc ggccgcgctc ggacagctcg cggcgcagct cgggttcgcc
    12361 gcacagccgt tcgatcgcct cgcgccaggc gcgcacgtcg agcgggtcgg gcaccagcag
    12421 ggctgcgccg ccggcgagct cgcgcagcgc cgggatgtcg ctcacgaggg cgggagtacc
    12481 cgccgccatc gcgtccagca gcggccggcc gaagccctcg accagcgacg ggagcaccac
    12541 cgcgagcgca cccgcctgga gccccgcaag ccgctcggcg ggcacgaatc cctcgtggcg
    12601 cacgcggtcc gtgaggcccc agtcgcgcac ctgccgaccg aacgccgcgg cggtctgcgc
    12661 ccgcgcgcga ccgacgacca ccaggtcgac gtccggcagc gcgcccagcg ccgcgagcag
    12721 cgtgtggagg ttcttgcgcc tgtagagctc cccggcgtag agcaggtagg gccgctcggc
    12781 agccaccggg tcggccggcg acagctccga cggcccctcc cccatcaccg tcgtcttccc
    12841 gcgcgcggcg gccacgtgct cctcgagcga cgcgcgcgtg gcctcgctga cgcacacgat
    12901 cgcggttgcc cgtgagcaga cgaacgggag cgccgcgcgg aagcggagcc gcttcgcccg
    12961 cccggtcagc tcggggtgcg tgagcgggaa gaggtcgtgc acgaccacga tcgacggcgt
    13021 ggcgagccgc cggaacggca gctccggacc ggtggcgagg agcgcgtccg cgcgcgccgc
    13081 gagccgcggc agctcgcgct cgcgccacag cgcccgcgcc gggagcgagg ctcggggcgg
    13141 ctcggccagc tcgacctcgg cgacctcgcg cagcgcctcc acgagcccgc gcgagtaggc
    13201 gctcagcccg ctcgcgccgc ctgccgctgc cgcggtgtct acgaggagcc gcgtcagtcg
    13261 gagtcggcct gcagcgcgga cttcagccgc aggacggcct tcgtgtggag ctgcgagacg
    13321 cgcgactcgg tcacgcccag cacctcgccg atctcgcgca gcgtcaggtt ctcgtagtag
    13381 tagagcgcca caaccagctt ctcgcgctcg ggcaggcggg cgatcgactc ggcgaggcgg
    13441 tccttcgtct cggtgaggtc catcgcgtgc gcggggtcga ccgcgtccgg gtcctggatc
    13501 gtgtcgagca gcgagacctg gtcgccggag gcgtccgaga gcgtccagag ctcgtcgagc
    13561 gcgatcaccg acgagttcga gatccgcgtg agcgcgtcct ggaagtcgtc catgctcatc
    13621 tcgagctcgt cggcgacctc ctggtcggtc ggtgctcggt ggagctggtg ctcgagcttc
    13681 gagttcgcgc gctcgatctc gcgcgccttg gcccggaccg agcgcggcac ccagtcgagc
    13741 gagcgcagct cgtcgatgat cgagcccttg atgcgggtca tcgcgaacgt ctcgaacttg
    13801 atctcgcgac ccgggtcgaa gcgctcgatg gcggagatca aaccgagcag tccgtaggag
    13861 atgaggtcgg cctcctcgac gtgcgcgggg aggccggtgg acatgcgccc ggcgacgtac
    13921 ttgacgagcg gcgagaaggc cagcacgagc tgctcgcgcg cgcgctcgtc gccgttctcc
    13981 ttgtatttgc gccagagctc cttgagctcg actgctttga cgttggtctg catcgccatg
    14041 aggccggtct ctccctgccg ccgctacgcg cgcgggtgcg cttccgcgta gacccttctg
    14101 aggcgtttcg actctatccg agtctgggtc cgagactcgc gtagcgcgcc cggcgaccgc
    14161 gcccgcacgg ccgcgtcgaa atccgtactt gcttcaacgc gccatttcgg ttacagatag
    14221 acacatggcc cctacacccc tcacccacac cctcgggggt gggcggggag gcatcctcac
    14281 gctcgcctcc gccctctgcc tcgccctgct tctggcgatc gccgcgcccg catccgcggt
    14341 cacgcacaag cgcgccgcca agaaggccgt cgccgccctc ggctcgtccc acgccagcgg
    14401 cgctgtcgtg gtcctcggcc tgccgaagcc gcttcgggcc ggcaccctcg tcacgcagca
    14461 ggggaagaag aaggtcgtcg cgcgggtcgg tcgtgagcgc gcgttcttct actacgagga
    14521 cgccgcgccg ctccagccgt tcccgcacgc cgggcgcgtc gcgctcgtcg gcgtcaagag
    14581 cggcaaggtc aggctcacgc gcgccctcac gcgcaggccg ctcctgaacg gccggctgcc
    14641 ggcgttcctc aagagcgtac gtgcgtacaa gaacgaggga tacgtggtct acgacagctc
    14701 gacgaagctg agcgcaccgg cgccacaggc gcccccggtg gggtccgaga ccaccacgca
    14761 gcaggatccg ttcccgatcg agccggtcgg ggtgaaccgc cggccgacgg ctttcgacca
    14821 gaacgtggcc gcgaagctga acagccccaa gcacataatc ctgacgggct cggaccccga
    14881 cacggcaccc ggcggcgaca gcgagggcac gctgctgacg ttcgcggtga ccaagctgcc
    14941 gaaccacggc tcgctctcgg gccagccgcc aagcctcacc tacacgcctg accccggctg
    15001 gtccggcccc gaccacttct tcttcaaggt caacgacggc gatctcgaca gcaaggaagg
    15061 gaaggtctcg atcaacgtcg tggcgcttgg cgccccgccg gtcgtgacca cgtcgaccgg
    15121 ctgcaccgag tacctggagc agaccgcggg cgtggcgatc gacggccagc tcacggcgtc
    15181 cgaccccgac gacacggtgc tcgacagcgc agtcgtccgc atcgcgagcg gcctggaggg
    15241 cggcgacaac ctgctcttca ccgatcagaa cgggatcacg ggcgcgtacg acgagcgcac
    15301 gggccagctg gacctggtcg ggacggcgag cgtcgcgacg taccaggccg cactgcgcac
    15361 cgtccgctac cagaacctct ccagcggcac gcccgcggcc tcgaagggga tcgcattcac
    15421 ggtcaacgac gccggcagcg actcggcccc cgcgaccaag gaggtctgca tcaccgaggc
    15481 cggcggtccg aacaaccggc cggtcggcga gacgagcgag ggcgtgctcg actacatcga
    15541 gaacgacggc gccgtctcgg tcgatcccgg gttcttcgtc ctcgatccag actcgagcca
    15601 gctctccggc gccacggtga ggttcaccgt aagccagccg cccgaggacg acaacggcaa
    15661 cccgatcggc agcccggtga acaacttcgc accggacgag gacgatctgg ccttcaccga
    15721 ccagaacggg atcacgggcg cctacgacga cagcacgggc gtgctgacgc tgagcggcct
    15781 ggcctcggtg gccgactacg aggccgcgat gcgctcggtc acctacgaga actcgtccga
    15841 ggatccctcc ccggcgccgc gcacgctgcg gttccaggtg aaggacgcca gcgggctgaa
    15901 ctcggtggcg agcaaccgcg gcgtcctggt cacgccggtc aacgacgctc cgacggccac
    15961 gcccagcgag ggcgcgtcct cctacaccga ggacgagccg gccagcgcgg tcgactcgca
    16021 gctcgacatc ggcgacgtgg acaatgacca gctccaaggc gcccaggtga agatcgcctc
    16081 gggcctccag tctggtgacg agctcgcgtt cgaggaccag ctcggcatca cgggcagcta
    16141 cgacgccgag accacggtgc tgacgctgac cgggaccgcc tcggtcgacg actacgagac
    16201 cgcgctgcgc tcggtcacgt tccgtcacgt cggtgacgac ccggaccccg cgcggagcgt
    16261 ggagatcgtg gtcaacgacg gcgagctcga ctcggcgacg gccacgaagg agatcgcggt
    16321 gacgcccgtg aacgacgcgc cggtcgcgac gaccggcgcc ggctcgaccg cctacaccga
    16381 gaacgacccg gccacgacga tcgacacggc cgtggcgctc tccgacgtgg acgacgacca
    16441 gctcgagggc gcccaggtga agatcgcctc gggcttccag ccgggtgacg agctcgcgtt
    16501 cgtggaccag ctcggcatca cgggcgagta cgacaccacc accggcgtgc tgaccctgac
    16561 gggcacggcg ccgatcggcg attacgagac ggcactgcgc tcggtcggct tctcgagctt
    16621 cgacgacgac ccggtcgcgt cgaagacggt cgagttcacg gtgaacgacg gcgacgacga
    16681 ctcggccgca gcctccaggg agatcgcggt cacgccggtg aacgacccgc cggtcgtctc
    16741 aacctccgat ggctcgaccg agtacgagat cggcggcccg ggcgtggcgg tggatccgca
    16801 gctcacggtc tcggaccccg acgacacgtc gatcgcgagc gccgaggtca cgatctcggg
    16861 cttcgaggcc ggcgacgagc tcgtgttcgt ggaccaactc ggcatcacgg gcacttacga
    16921 caccgagacc ggggtcctct cgctcaccgg cacggcctcc gtgggcgact acgaggccgc
    16981 gctgcgctcg atcgagttcg ggaccaccaa cgagacgccc tcggcgtcac gctcgatcgg
    17041 gttcacggtg aacgacggcg acgccggctc ggccgcggcc tccagggcga tcgacctcgt
    17101 cgaggcgaac gacgcgccgg tggtcacgac cacgacgggc gactccacct acacgctggg
    17161 cgactccgcc ggcgtggtgg tggacgacct gctgaccgtc accgacggcg acgacacgaa
    17221 cctggagagc gcggtggtgc ggatccaggg cggcgagccg ggcgacgagc tcgtcttcac
    17281 cgaccaggcc gggatcacgg gcaccatcga cgagacgggc ttcctgatcc tgaccggcac
    17341 ggcctccgcc gccgactacg agacggcgct tcggtccgtg cgcttccgca acacgaccgc
    17401 caccgaggcg gggacgcgga cgctcgagtt ccaggtgaac gacggtcgcg acgactccgc
    17461 gaccgcaacg aagggcgtcg ccctggtcgc cgccccgcca cccgcgccct gacccgtccg
    17521 ccaggctctg acccgcgccc cgcgcctgac ccatccgccg cgccctacgc gcgcggatgg
    17581 gtttcggcgt agaccctcct gagtcgcttc gactccaccc gcgtgtaggt ctgggtcgtg
    17641 ctgagggtgg cgtggccgag cagctcctgg atcgtgcgga ggtcggcgcc gccctcgagc
    17701 aggtgcgtgg cgaacgagtg gcgcagcgtg tggggcgaca cccccgcccc ggccgccgca
    17761 cgccgggttg ccacggccag ccggcgccgg acgtccgagg tgctgagacg gcggcccgac
    17821 ttcgacagga acagcgccgg gtcggggtcc gcccggttgg caagccgcgg gcgggcgcgg
    17881 gcgagatagc gctcgacggc gcgccaggcg ggctcgccgg cgggtatcac gcgcgtgcgg
    17941 tcgcccttgc cggtcacgcg cagctcctcg ccgtcggggt cgaggtcgcc ggcgtcgagg
    18001 ttcacgacct cctcggcgcg caggccggcc gagtaggtca actcgaacat ggcgcggtca
    18061 cggagctcga gcggctcgga cgccggcatc gcgtcgagga tggccgccac ctcgtccgcg
    18121 cggaggacgc gcgggaggta gctgtcgcgc ttcgggctcg agaccaggtc ggccgggctc
    18181 cccgcgagct cgccgcgctc gaccaggtgc cggtagaagg cacggatcgc cgccagcttg
    18241 cgcgcgaccg tgctgcgcga gttgccgcgc tccgagagga ctccggcgaa gcggcgcagc
    18301 gtgcggtggt ccatcgcgtc cggctcgtgc ccctgggcgc tcgcccactc ggcgaactgc
    18361 ccgaggtcca cgccgtaggc gcgccgggtc ttctcggcca tgccccgggc ccgcatcgaa
    18421 ccgtcgaagg cggccagagc gctggaccag cgcggcgtcg gctcgtagtc acggggggcg
    18481 ctcggggcca cgccccgagg ttctacttct tggcccgctt gaccttctcg cagcccttga
    18541 ccttgtcggc ccggtcgacc gaggcggagt ccttcttccc ggccccgcag tcgacggtct
    18601 ccttcttgcc gtttcgggcg ttgatcgagt cgtcgcccga gccgcctgcg tacttgttga
    18661 cgtcgcccga gccggtgagc ttgtcgttgc ctttgccccc gtccagcgag tccttgccct
    18721 tgccgccgtt cagggagtcg tcgccgccgc cgccgaacag cttgtcggcg ccgtcctcgc
    18781 cgaacagctt gtcgttgccg ccggccccgt agagggtgtc gttgccggtc ccgccgaaga
    18841 gcgtgtcgct gccgcccgcg ccggcctgcg cggaggcgat cctcgccttg acgttgcaga
    18901 ggtcgccgaa gaccgtgtcg ttgccgccct gcgcatgcac cacgtcgttg ccgagcaggg
    18961 cgcagatgat ctctccggcc gcggtcccgt cgaccgtgtt gttgccgttg tcggcgccga
    19021 acttcgtcgg gatcgtcggg tcgttgggct cggggtcgct cgagtcgggc accccgtccc
    19081 cgtcggagtc cggcggcgcg ggtccgggcc cgggcgcggg gttgcagccg ttggccaggg
    19141 tccccttctc ggtcttgcac tggtcctggg cgtcgggcac gccgtccccg tcggagtcca
    19201 gcggcgcgga cttcgcgtcg gcgccggagc agtcctcgtc cacgccgttg tccggcacgt
    19261 cggtcgcgcc gggcttgatc gcggcgttgc cgtcgttgca gtcgcccggg gcggggccgg
    19321 cgttcacccc gtcgccgtcg cggtccggat cgcacgcgtc gccgagcgag tccttgtcga
    19381 ggttctcctg gttcgggttc gcgacgagct ggcagttgtc ggcgctcgtc ccgccatcgg
    19441 ggacgccgtc gttgtcgtcg tcctcgtcct cgatgtcctt gcacccgtcg ccgtcggtgt
    19501 cccctccgaa ggtcgagccc gcagggcagt cgtccgcccc gtccccgaag ccgtcgttgt
    19561 cgtcgtcgtt gtcgcacgcg tcgccgccgt cgttcacgcc gtcggtgttt tcctgggcgg
    19621 cgttgggcac gttcgggcag ttgtccctcg cgttggggat cccgtcgccg tccgggtcgt
    19681 cctgcgtgtt gtcgcaggcg tccccgaggc cgtccccgtc ggtgtcctcc tgctcgggat
    19741 tggcggcgaa ccggcagttg tcgctcggat cgccgaagtc gtcgaccccg tcgttgtcgt
    19801 cgtcggcgtc gcacgcgtcg ccggcctggt cggcgtcgtt gttggcctgg tcggcattgg
    19861 gcacgaactg gcagttgtcg gccgtgttgg tgaccccgtc accgtccgag tccgaggggt
    19921 tcgcgccgct gtcgcaggcg tcgccgatgc cgtccccgtc cccgtcctgc tggcccgggt
    19981 tgaagacgaa ctggcagttg tcgatcggct cgccaacctc gccgaagtcg tcgacgccgt
    20041 cgttgtcgtc gtcggggtcg caggcgtcgc cgagcgagtc ctggtcgttg tccgtctggt
    20101 tcgggttggc gacgaaccgg cagttgtcgc tcggctgccc cgcgtcgagg acgccgtcgt
    20161 tgtcgtcatc ggcgtcgcac gcgtcgccct gggcgtcctc gtcgttgttc tcctgctcgg
    20221 tgttggccgc gttcgggcag ttgtcctgcg cgtccgggat cccgtcgttg tcggcgtcgc
    20281 ccgtgacggc catggccacg tccgtccgca gaaggggctg gtcgaggagc gacccggcgc
    20341 cggcggggac cgccagccac gccgcgacca gggcggcgag gagagctatt cggagggtgc
    20401 caggcatggg gtaacggcgc aaccggcgat caaaagtact ccccttcgcg gacgaggtca
    20461 acgacctaag ctcccgatcc tcgtgggatc gttcttcatc gacgtgcagg acgggcgcgt
    20521 ggccaccagg acccagctcg aggcggccgg ggtcgtgtcc gccgacgtgg ccggtcctcc
    20581 gccgcgcccg tggcaccccg tccaggggcc cggcgacgcc tccatgatgt ggtacgccgt
    20641 catgcgcaag cgactgcgcg gggtattcat cggcacgctc tgcctccgcc acacgggccg
    20701 ccaggccgcg ctcgaggcgg agggctggga ggagatcccg gtggagcgga tcggcctcgg
    20761 ggcgtgacgg aagcgccggg ccggcgggac aggtcgtggc ggcttccgtt cctcgacgat
    20821 cccgactggc agatgtgggc cggcgagcgc gcggcgctcg agggagtcct gtcggcactg
    20881 aggccgcgcc tgtcgatcga gatcgggacc gcccagggcg gcagcctgcg gcgcatcgcg
    20941 gcgcactcgg gcgcggtgca cgcgttcgac gtcgatccgg ccgttgcgga ggtcgtgcgc
    21001 gcggtcccga acgccatcgc ccacgtgggc gactccgcgg cgaccctccc gcccgtgctc
    21061 gccgagctcg cggccgccgg ccacaacgtc gacttcgcgc tcgtcgacgg agaccacagc
    21121 gccgcgggcg tgcagcgcga cgtgcgggcc ctgctcgcct ccccggcctg ctcgcggacc
    21181 gtggtgatcc tgcacgactc ggcgaacgag gcggtgcggg acggcctcga ggcgctcggc
    21241 ctccccgacc acccgaaggt gcgcgtctgg atgccggact tcgtccccgg ctacctggtc
    21301 ggcgcgcgac acgagcgcgc gggcgagatc tgggaggggc tggcgctcgc cgtcctggac
    21361 gtggacgccc cgccgggtcc cgccgtccag gacgaggtgc acgagaacgt gagcgcggtg
    21421 taccgcgcct tccgcgacgc aaaggcgcgg gccgcgggct cccccgcccg gccctagaac
    21481 gaccgagcac cccgcggtgc ggggtgctcg gcctccagtc attgcgccac gccccagcca
    21541 gggcggggac ccgactatct cgggatcagc gcttgcgctt cgtgaacgtc aggttcggcc
    21601 cggtcggcgt acctgcggtc gcgttggtga cgttgccgtc cacacggttg tcaccccacg
    21661 acttcagctg gcccgagtcg agctgctgga gcccgacctg gttgccggtg acctcggtct
    21721 gggacatccg ctggagggcg ttgctgccgc gcacgaacac cccgacgccg ttgctgtcgg
    21781 tgatgctgtt gcggaagccc gagagctggg cgcccacgtt gaacgcgccc gggcctgcgg
    21841 taccgcagtt cacggcgtag ttgaacccgg catcgaagcc ctgcgacgag accgtcaggc
    21901 cgcagccgtt cgagtcgaag tcgctcttgc gcaccgtcgc cttcgcaccg ccggcaatgc
    21961 tctgcgggtg aacgaacacc ccgttgccga ggttgtgatg gacgtccacg ttcgacacca
    22021 cgagccgcgc ggtgttgttg gccggctcga agtcgattgc gttacggacg aagccgaaga
    22081 tctcggagtc gaggaccttc acgctcttcg cctgaaggac cctgacgccg ttcagtccgg
    22141 tgccgattcc gttgatgtcg agcccgcgga gcgtgacgtt gtccgtcgcg gccgcgttga
    22201 cgacgatcgc gttcgtgccg ctcacgagga caccgccctc ggtgtagtgg cacttgatgg
    22261 cgagcgactt ggtgatcgtc actccgccga agccgcccgg gtcgaggcag ttgatctcgc
    22321 ccttcgcggc ggtcttcgag atcgcacccg caaaggtctt gcagggcgcg gtccggctgc
    22381 atgggttggc gtcgtcaccg actccggaga cccatgttcg agtagcctgg gccgaggccg
    22441 aggcggggag tgcgagtgcg atcagcacca gcactggaga cagcttgagg gtatgtaggg
    22501 gtaggcgcat aggcacacac taaccgcgtg ggccgcccag cgccagtttt ttcgctaacg
    22561 acttttccat ggaccaaaac ggcacgaaac cgacactcga gatcatcatc gtgacctcga
    22621 atggggcgaa gcagcacatc cgcgcctgcc tccgttctct cgagctcaac cccctcacga
    22681 gcggctcgat gctcgtccac gtcgtggaca acgcgagccc ggacggcacg ccggccgtgg
    22741 tgcgggagga gttcccgtgg gtcaagctga cggcgctcga ctggaacgcg ggcttctgca
    22801 tcggcaacaa cgtcgggatg cgggaggcga gcgcgccata cgtgctgctg ctgaaccccg
    22861 acaccgaggt ctacgaggga gcgctcgacc acatgatccg cgtcatggag gagcgccccg
    22921 acgtgggcat gtcgagctgc cgcctcgccc agccggacgg cacgttcgac cacgccgcga
    22981 agcgctcgtt cccctccccg ctgggagccc tggccgagtt cgtgggggtc gggcgcggcg
    23041 gacgcatgcc gggcaccctc gagggcgtgg ccgactaccg ggcccacgac atcggcgagt
    23101 acgaggaggg cgaggtggac gccgtcaacg gcgcgttcat gctctgccgg cgggaggcga
    23161 tcgaggccgt ggggctgctc gacgagcagt actggctcta catggaggac ctcgactggt
    23221 gctaccgctt caagcaggct ggctggaagg tctggtacga cgggcgggtc accgtcatgc
    23281 acgtcaaggg cgggaccacg aaggtcaagc gccaccgcgg gctgcgccac aacacggcct
    23341 tccaccgcgg gatgggacgc ttctaccgca agttctacgc cggcgagcgg ccggtgctcg
    23401 actgggcggt ctacctcgcg atcggggtca agctggcggt ctcggtcgcg cgcagcaccg
    23461 tggcgcgccg gcggctcacc tgacgcccat ccgtccccgc caccagccca ccgtggcgcg
    23521 gatcccgtcg cgcagcccgt agcgggggct gaagccgacc tcgtcgcgca gccgggcggc
    23581 gtcgccggcc acgaggtcgg gctcgctcgc tccgcgctcc acctcccccc agccgacgag
    23641 atcggggcgc ccgacctcgt ccgcgatcgc cgccaccacg tcgcgcacgg cgaccgggtt
    23701 cgccgacgcg atgttcaccg ggccggtcac cgcatcggcg tcgagcagcg ccaccagcgc
    23761 ggccgccacg tcgtccacgt gcatgaagtc gcgccgctgg acgccgtccg tcgtcggcgc
    23821 gcgctcgccg cgcagaagag ccgtcgtgac cgacgggacc agccgcgcgg gtgcctcgtg
    23881 cggcccgtac aggaagaaca cccggccgtg cgcgaccgag gctccgctcg cccgtccgag
    23941 ctcctccagg ctcgagccga gcgcctgctt ggtgcggccg tagagcgtcg ctggcgcgag
    24001 cggggtgccc gcctcgctca accagcccgc gctccagtcg tactcggcgc aggtgccggc
    24061 cgtgaccacg cgggcgcctc gcgccgccgc cgcctcggcc agctcgaggc tcgccctcag
    24121 ccaggcctcg ttcgcgggcg aggtccagta ggcgccgggc gtggtctccc acgcgaggtg
    24181 cagcacagcc tcgggcgcca cctccgccac gagggcggcg gcggccccgg gcgccagcag
    24241 gtcgcgccgg tgccactccg cggggcgcag cgcccagtcc ggagcggggc cacgacccac
    24301 cgcgtgcacg tccgcgccgc cgtcgagcag agccgcgagc gccgggcggc cgatgaagcc
    24361 ggcggcgccc gtgaggagga cccgccggct cagctcggcg acccggtcca gtccgggtag
    24421 ccggcgtcgc gatcggccat gagctccggc cgcacgcggc cgggccactc gatggcgaac
    24481 gccgggtcgt cccagcgcac gccgcgggcc gccccgggca cgtgcggcgc gtccatcatg
    24541 tagaggagct cgacgtcgtc ggtcagcgtc aggaagccgt gagcgcagcc cgggggcacg
    24601 tgcagggcac ggcggttcgc ctccgagagc tccaccccgg cccacgcgcg gaagctcggc
    24661 gagtccggcc tcaggtcgag tgcgacgtcg tagaccgccc cgcgcacgca gcgcacgagc
    24721 ttggcctcct cgtgtggagc aagctggagg tgcatcccgc gcagcgtgcc ggcgtgccgc
    24781 gtgtacgagg ccgagcactg cacccactcg gtcgggagcc cgtgctcggc gaacaggtcg
    24841 cggtcgaccg tgcgtgcgaa gagcccgcgc tcgtcctcga ccggctctgg cacgaccacc
    24901 acggcgccgg acaccgcgga tggctcgaac gtcactcgat gacctcgaag ctgggcgtgc
    24961 gcactacgaa ccggccgccc cactcgcgaa cgtgcgccat ctgctcgacg atctcgtcgc
    25021 gcaggttcca cgggaggatg aacacgaagt ccggccgcgt ccgggcgatc tcctcgggcg
    25081 cgtggatcgg gatccgcgtg ccgggcagga acttgccctg cttgtgcggg ctcaggtcga
    25141 cggtgtagtc gagcatgtcg ggtccgatcc cgcagtagtt caggagcgtg ttgccctttg
    25201 caggggcgcc gtagccggct atcgaggcgc cctgctcctt cgcgtcgacg aagaaccgca
    25261 gcacctcgcg cttcgtggcc ttcacgcgct cggcgaacgt ccggtagacc tcgaggtcct
    25321 cgtagccggc ctcgcgctcg cgctcgacca gctcggtcac ccgccgcgtg accggctcgt
    25381 ggtcggactc ggcgtgccgg gcgtagatcc gcagcgagcc gccgtgcgtg ggcagctcgt
    25441 ccacgtcgaa gagctccagc ccgtgcgcgg cgaacacccg ccgcacggtg aggaacgaga
    25501 agtacgagaa gtgctcgtgg tagatcgtgt cgaactggac gtggtccacg agctgctgga
    25561 ggtgggggaa ctccatggtg atcacgccct gcggcgcgag cagcaccttc atccccgcca
    25621 cgaagtcgtt caggtcgggc acgtgcgcga gcacgttgtt gccaatcagc aggtccgcct
    25681 gggcatccgg cagcaggtcg cgcgcggtat tgacgccgaa gaaccgctgc acggtcggaa
    25741 cgcccttgac ctgcgcgacc tgggcgacgc tcgccgccgg ctcgaccccc agcacccgca
    25801 cgccccgcct ggcgaagaac tggagcaggt agccgtcgtt cgaggcgacc tccacgaccg
    25861 tcgagcccga gccgatccgg aagcgctcga tcgcgtagtc cacgtagcgc tggcagtggt
    25921 cgagccagga ggttgagtac gacgagaagt aggcgtagtc gtcggcgaag atcgcctccg
    25981 gggtctcgaa gtcctggagc tggacgagga agcagtccgg gcagaccagc gcccggagcg
    26041 ggtagaacgg ctccatgccg ttgagctgct cgggcgacag gtagtcgttc gcgatcggcg
    26101 acatcccgag gtccgcgaag ctcgtgcgcg ccaccgcccc gcagaaacgg caggtcgtgg
    26161 agccaacccc ggcgatggag actccttcag tggtcaccga cgtccccgtg gacgaatccc
    26221 ccacgccccg caggttagag aagcgcgctt atcctttcgc cggatgcccg gcggatcgtg
    26281 cgtggagccc tgaccgccac cggccccgcc gtgtcggcgg tcgtgctgac ctacagccgc
    26341 caggacctgc tccccgactg cctgagctcg ctggcgacgg cgctcgcgga ggtgcccgag
    26401 ccgaccgaga tcgtggtggt ggacaacggg tcccccggga acgtggcaac cggacgggtc
    26461 cgtgagtggt gcccggacgc gaagatcgtc gtgctgaccg agaaccgcgg ctacgcgggc
    26521 gggatcgtgg agggaatccg gcgcgcccgc ggggagtgga tcctgaccgt cggcgacgac
    26581 tcgaccgtgg acgcaggggc cgtggtggcc ctgctggcgg cgggccgcag cgcgcccgac
    26641 gtcggatcgg tcgcggccaa gatgctgttc gcggaggccg cgggcggtgc cgtgatcaac
    26701 tccgccggcc tcgagatcga ccggctgggg atcgcggccg accgcctcgt agggcagccg
    26761 gccgacgccg gcgagcgcga cgtgacggag gtgttcggga cgagcggcgg cggggccctg
    26821 taccgggcgg cgatgctcga cgcgatcggc tcgttcgatg cgagcttcgg gctctacctg
    26881 gaggacgccg acgttgcctg gcgcgcccgc cgggcgggct ggcgctgcct gtacgtgccg
    26941 gaggcgatcg tctaccacca ccactcggcc acgaccgggc accgctcgcc cggcaagtac
    27001 cggtacgtgg gtcgaaaccg ggtgcggctc gtggcgaaga acgcgaccag gcggcagttg
    27061 ctgcgttatg cccttccgat gatcggctac gacctcgcgt acgtcgcgta cgcggccctg
    27121 gcgaaccgca cgctggcgcc actgcacggc cggctcgagg gcctgcgtca ctggcgggcg
    27181 gaccgccgcg ccgcgcgggg ggaggtggag gtcgagctcg cccccgtcct gggcctgcgc
    27241 gccgcgctgc gccgcaaccg cgcctggcgg ctcggctcag gcggatgagc gcgcgcgaga
    27301 cgcctccgcc gcggctgtcg gtgccccgct ccgcgggcgt cttcctcgcc gccacgacga
    27361 tctcgcacct ctcccagttc gtctggctgg cggccggcag ccgggcgatg tcgcacgacg
    27421 acttcggcgc cgtgctcgcg gcccaggtcc tgtacgcggt cctccagatc gcgatcgaca
    27481 acggcacggc gcaactcgga gcgcgcgagg cggcacgcgg cgcgatgacg gacctcgacc
    27541 tcggccggct cgtgcgcctg cggctggcgc tcaccgcggt cgccgtcccc gccgggctcg
    27601 cgatcggggc ggcgtcggcc gggctcgcgg ccaccgcccc gttcatggcc gcgctgctcc
    27661 tgttcgccct cgtcaacgtc tggcggccct acggccaggg cgacccgcgg ccgtgggccg
    27721 gctacatgtt cggccgctcc gtgcttccgg cggcggtcgc gtccgcgtac gcgctctccg
    27781 gcggctcgtt ccccgtggag ctcgccgggg ggctcgagtg cgtggtgctg gtggtgctga
    27841 tcgccgcgac cggccagcgg ccgctccgcg agctgcgcct ggcgctgcgc agccgcggca
    27901 tgccgtggcg gccggtgctc tcgatcggcg tgccggcgat gctccagcag gccagcgtgg
    27961 ccgcggggac catcgggctg agcgcgggcg gcgcgccgcg tgccgcgggc gcgctcgcgg
    28021 cctgcgtgcg cctgctgacc gggctcaacg cgctcaacgg gatcgtggcg acggcgatgt
    28081 tcccctcgct cgccgagcgc ggaggcctcg gggcgggcgt acgacgcgtc gtgctcgggc
    28141 tgcggatgac gaccgcggtg gcgttcgcgg cgacggcggc cgtctgcctg gtcgccaagc
    28201 ctctggcgca gaccctgctc gaccggacct cggacgcgat cgagtcggcg ctcgtcctga
    28261 ccaccgccgg cgcggcgggc atggggacga tcgtgatgct gacctacctg ctcgtggctg
    28321 ccagcgggga gcggcgcgtc ctgcccgcct acgcggccgg cgcgggtgtg acactcgcgg
    28381 gcgtgacgct ggtggtggcg agcggcggcg agcgcgtgga cacgatcgcc gcgctgctcg
    28441 tggccggtca gctcacgacc gcggccggcc tggtgctgcc ggtcgcgcgg agcctgccgg
    28501 actaccgcgg cccggcgcgc gcggccctgg cgggcgcggt cgcgctggcg gcggtggccg
    28561 tgaccgccgt cgtcaccgac gcggccgtcc cggcgggggt cgtgctcgcc gcggtcgccg
    28621 tcgcggtcgg gtggggcggc gcccggccgc tcgtgggggt cctgcgaacg cgcggcgccg
    28681 gtccgcgacc gaccgcggga tcacgatgat cgacgcggtg atcctcacct ccaactcgcg
    28741 cgacatggcg ttgcgctgcg tggcccacac gaaggcggac ccaaccgtgg accgcgtgtt
    28801 cctggtggac aacgcctcgt ccgacgacac cgtggcggcc gtccgcgccg agcaccccga
    28861 ggccgaggtg atcgccctgg ccgagcacac gggactcgcg gccgcgctga accgcggcgc
    28921 cgaggcgggg tcgaacccgt acgtcctcta cctgaacgac gacgtcttcg ccgcccccgg
    28981 agccgtggcc acactgctgg cggcgctcga gggccggccc ggagcggtcg cggcgggagg
    29041 gcggctggtg aacgaggacc tgacgacgca ggaccagtac cgcccgcgcg acttcccggg
    29101 cccggtcacg atctcgctgc ggctgctcgg gctcgaccgc ctctggccgc gcaacccgtt
    29161 cacgggccgg cacctgcgcc acccgctcga cgacgagacc acggtcgagg ccgaccagcc
    29221 cgccggcgcc tgcctgctcg tgcgccgcga ggcggtggag gcggtgggcg gctgggacgc
    29281 gcgctactgg ttctggtacg aggacgtgga cttctcgcga cggctcgccg acaccggcgg
    29341 ggccacgctg tacgtcccca gcgctccgtt caggcatctc gggggcgcca gcaccacccg
    29401 ctggtcgctt cccgagatgc accgccgcac ctactacggg atgctcctct acgccgagac
    29461 gcacttcgcg cgggcgggcc gcctgctcgt ggcggcgacc atggcggtcg tctgcctgct
    29521 gcggctcgcg gccacccgcg gcggcgccgg cgccggcgga tatagcggct gcctcgtcca
    29581 ggcgcgcgcg cgggcggcgg g
//
LOCUS       DASVPL010000036         9052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_316710, whole genome shotgun sequence.
ACCESSION   DASVPL010000036 DASVPL010000000
VERSION     DASVPL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316710"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VF517_01965"
     CDS             complement(<1..1171)
                     /locus_tag="VF517_01965"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8051726.1"
                     /translation="MRRTLLLAVGVLGVLLVVVGPALSVRPYQPRALDFELAPPALSA
                     AGAGPVESPVVRAPKRFNLVGMRWNGSAEPRISMRVRRAGGGWSHWVKVGADGEHGPD
                     PGSAEDHPGGTSDPVWAGDADELQYRLSRPVSGLRLHFVNTMGSATAADRAKTALRRA
                     ANAGLRTAARIAGVADADAQDPQPEIAPRSTWENGQCTPRSAPAYREVKVAFVHHTVS
                     ANEYTAEQVPAMLLSICRYHRNSNGWNDMGYNFLVDKYGRLWEGRAGGIDRPVVGAHT
                     EGMNTQSFAVSNIGDHSTLPAGEPAMEAMTRLIRWKLPLHGQPTSGTVTVVSNGGGGS
                     RFAAGEEATLERVSGHRDANSTACPGQALYDQLPQLRERVAGAPVAARPAPAVSMT"
     gene            complement(1257..2111)
                     /locus_tag="VF517_01970"
     CDS             complement(1257..2111)
                     /locus_tag="VF517_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8051727.1"
                     /translation="MSVRTGEFVHDGHRLVYDEYGSGPRPFLLLPGLMLPRLMHRPLA
                     QALAERGHRVITLDLLGHGESDQPRDMWRYSMSIFGEQAIALLDHLEIERAVVGGTSL
                     GANVTLEAAVREPDRMQGLVIEMPVLDNALLGCALAFTPLLVGLTFGAPVARVLSQVA
                     RRVPRGLANLTDVGLDWASRDPAPGSAILQGLFFGRIAPPRHERAGIETRSLVIGHGR
                     DPIHPFSDSDMLVRELRNGRLIEANSILELRLAPERLTGEIARFLDECWRRPAGAVGE
                     PPGRAATA"
     gene            2235..3782
                     /locus_tag="VF517_01975"
     CDS             2235..3782
                     /locus_tag="VF517_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8051728.1"
                     /translation="MEVSSLPHPGSGLLPRSRRVLAAFGDERLVDQLRRGNEVAFEVL
                     YDRHHRAILSFCRHMLSSRDEAEDAVQQTFISAYNDLAGSDKQLKVKAWLFTIARNRC
                     LSILRARREQAAELDDIPTAGLSQQVQERDDLRRMLADLRELPEEQRAALVLSELGDL
                     SHAEIAGVVGCETMKVKSLVFQARSSLLESRQARDIPCQEIREQLATLRGGALRRGPI
                     RKHLNACPGCREFREDVRRQRAALAALLPVVPSAGLKGGVMGAIGLGGGGAAGGGALG
                     LGAGGSALAGTGAAAGGTAGLGGIVSIIAGSGVTKVAVAAVLATGAAAGGVAVVSDAG
                     SSSSGGGDHGSGTPMAHRQPGDIARPSKFSSVGIATPKGEVATVKRHDPSKESPAAGV
                     FIGNNANPTPGEPRGNARGKDSKDDGYYFVDGPGRSASRGNSHGANGTRARARGNGNA
                     RGNGYGSRPVPPRSSRSPNAGQPAPPTTPPPAAAPSGGNGADKAPKEPKGARGGKGPK
                     LPRLPLP"
     gene            complement(3789..5939)
                     /locus_tag="VF517_01980"
     CDS             complement(3789..5939)
                     /locus_tag="VF517_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8051729.1"
                     /translation="MLDAIAGLAQRRARTVVVVALLAAVLAGAAGGSVADRLGPFSAE
                     DPGTESIEVAERLEAATGVASDGNVVAIVTPGGPVRSDAGRARVAQVARKLAADPGIG
                     AVRSPFGRGGSESMVARDGRSAFVVASLRSAAKEEDVADRVERTLGDARGVRLGGGAL
                     AARAANATVEEDLRRAEMLAFPILFLLSFWFFRSLVAALLPLLVGGLSIVLTFLVLRV
                     VSEGVDLSVFALNLVTGLGLGLAIDYSLFMVSRYREEIAVSGPGPEALARTVRSAGRT
                     VLFSSLTVAAALASLLVFPQRFLYSMGVGGAAVALIAAVVAIVVLPAVLALLGERVNS
                     LSPRWLRRAAEAEAGDNRSGGWYRLSRFVQRRPGRIALVASIVLLAAGTPFLGASFTT
                     VGPETLPKSSEPRQVFDELNVRFPANQDAPLVVAAETRDPKQAAALARRIRSVDGIAS
                     VAAPRPVEPGLWQIDAVSRTHYTAPASEDLVRDVRSLPGPVEFGVTGQAARFVDLKSS
                     LGDHIPYAVGLLALTTILILFVMTGSVVLPIKSLAMNLLTVSAAFGFLVLVFQDGRFE
                     GLLGFTSPGALDATQPLVLFATAFGLSTDYGVFLLARIKEARDAGASDSDAVAIGLQR
                     TGRIVTAASLLFAVAIGAFATSQMIFIKELGLGTAVAVLVDATIIRALLVPALMQLLG
                     HRNWWAPRPLRRLHARFGIDEGPAAPPPAGAAAG"
     gene            complement(5944..6489)
                     /locus_tag="VF517_01985"
     CDS             complement(5944..6489)
                     /locus_tag="VF517_01985"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8051730.1"
                     /translation="MSDLTPTARVILGMIRLGKRTGYEIKQLVDISTRFFWTASYGQI
                     YPELKRLEEGGLITGEPDPAGGRARTVYTLTPAGEAALEAWLDSRDQLVWELRDESLL
                     KLFFSGERDRDALREHMREAGRRSAEMAERLRALAPDLDDPDPGPMMTLRFGIELNEW
                     MADWWARAEERAGRPADPRRR"
     gene            6621..7610
                     /locus_tag="VF517_01990"
     CDS             6621..7610
                     /locus_tag="VF517_01990"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEX8051731.1"
                     /translation="MNNPPPNWYPDPRGEAELRYWDGSQWTEHTHSGQAAAAPASPPP
                     AQAPPPQAPAPAGPPAAGTPTAAGGYHQPAYQQGAAAPAGGGSTGGSKLPLIIGGALA
                     AVALVVVLVLVLAGGGGDDGGGEEAKVEDRVKEAITGKEPEGCEDFLTTSFVQKSTGL
                     SGSEGVEACKKTRGQNTVSDAEVKDVKVTGEKATATAIPGSGPLEDEELEISLVKQGD
                     EWKIDDLDRPSVAQGDDAERAIINTVLNFGSSEGVKACDYLSYAGLQRLGGRSGCESS
                     FKEGQPANYSPQDISVTGTTATMVVEERRQNKTIKFTLSREVGTWKIDSFEQQ"
     gene            7615..8544
                     /locus_tag="VF517_01995"
     CDS             7615..8544
                     /locus_tag="VF517_01995"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEX8051732.1"
                     /translation="MTDAPPNWYPDPSGAAQLRYWDGRQWTEYTQGGPAVAPGQQPAP
                     PPSAAPTAAGAVPGAGPAPGGGSTGGSKLPLIIGGALVAVALVVVLVLVLAGGGGEEG
                     GGEEAKVEETIEQVATSKDPAVCEERHTVSYLQKATGLAGGAAVQLCEESTPENDVTG
                     VEISDVEVSDTSAEAKAQIEGGDYSGETLEISLEKQGDDWKVDDLDRPKVASGAAAEK
                     AVQTTVLNFGSAEGIAACDYLSYPGLERLGGRSGCEKSFKEGQPANYTIQSVTVSGTS
                     ATTVVEEARQGKTIEFKLSRELGEWEIDSFAQR"
     gene            complement(8489..>9052)
                     /locus_tag="VF517_02000"
     CDS             complement(8489..>9052)
                     /locus_tag="VF517_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051733.1"
                     /translation="RGTTVNLLAVPLSVTSILGAMLLFTMAPAMRPPQRIALLAVFAL
                     LGGLTAMLIVRVAIGALPGSFAALTLVASLAIAAMAFTSAGIMSAVGPSGTMLSFLIF
                     LMVANPASGAASAPELLPDPWREGGQLLPAGAAATGLRNVAYFDGTSLAKPLAVLLAF
                     AAGGALVLVTTAARTSRSPTPPARGRA"
BASE COUNT         1375 a         3314 c         3296 g         1067 t
ORIGIN      
        1 gcgtcatcga gacggctggc gctgggcgcg ccgccaccgg cgcgccggcc acgcgctcgc
       61 gcagctgcgg gagctggtcg tagagcgcct gccccgggca cgccgtgctg ttcgcgtcgc
      121 ggtggccgga gacgcgctcg agggtcgcct cctcgccggc cgcgaagcgg cttccgccgc
      181 cgccgttcga gacgacggtc accgtgccgc tcgtgggctg gccgtgcagc ggcagcttcc
      241 agcggatcag gcgcgtcatc gcctccatcg cgggctcgcc cgccggcagc gtgctgtggt
      301 cgccgatgtt cgagaccgcg aacgactgcg tgttcatccc ctcggtgtgg gcgcccacca
      361 cgggacggtc gatgccgccg gcacggccct cccagagccg cccgtacttg tcgacgagga
      421 agttgtagcc catgtcgttc cagccgttcg agttgcggtg gtagcggcag atcgacagca
      481 gcatcgccgg cacctgctcg gcggtgtact cgttggcgct caccgtgtgg tgaacgaacg
      541 cgaccttcac ctcgcggtag gcgggtgccg agcgcggcgt gcactgcccg ttctcccagg
      601 tcgagcgcgg ggcgatctcg ggctgcgggt cctgcgcgtc cgcgtcggcc actccggcga
      661 tccgcgcggc cgtgcgcagc ccggcgttcg ccgcccggcg cagcgccgtc ttggcgcggt
      721 ccgcggcggt cgcgctgccc atggtgttca cgaagtgcag ccgcaggccg ctcaccgggc
      781 gcgagagccg gtactggagc tcgtccgcgt cgccggccca caccgggtcc gaggtgcccc
      841 ccgggtggtc ctccgcgctg cccgggtcgg gaccgtgctc gccgtccgcg ccgaccttca
      901 cccagtggct ccatccgccg cccgcgcggc gcacgcgcat ggagatccgc ggctcggccg
      961 agccgttcca gcgcatgccg acgaggttga accgcttcgg cgcgcgcacg accggagact
     1021 cgaccggtcc cgcgccggcg gccgagagcg ccggtggcgc gagctcgaag tcgagggcgc
     1081 gcggctggta gggccggacg gacagcgcgg gccccacgac gacgagcagc acgccgagga
     1141 cgcccacggc gagaaggagg gttcgccgca tcacgtggtc ggaacacgct agccagcgga
     1201 gcgttgcggg gctgaaaatc ctgttgcgag gggcgtgtcc gcccgcggac tggcgcctag
     1261 gcggtcgcgg cgcggccggg cggctcgccg acggctccgg ccgggcggcg ccagcactcg
     1321 tcgaggaagc gcgcgatctc gcccgtgagc cgctctggcg ccagccgcag ctcgaggatc
     1381 gagttcgcct cgatcagccg gccgttgcgc agctcgcgga ccagcatgtc ggagtcggag
     1441 aacgggtgga tcgggtcgcg gccgtgcccg atcaccagcg aacgagtctc gatccctgct
     1501 cgttcgtggc gcggcggcgc tatccgtccg aagaagagcc cctggaggat cgccgacccc
     1561 ggtgcggggt cgcggctggc ccagtcgagc ccgacgtcgg tgaggttcgc gaggccgcgc
     1621 ggcacgcgcc gcgccacctg gctgagcacg cgggcgaccg gcgcgccgaa cgtgagcccc
     1681 acgagcagcg gcgtgaacgc gagcgcgcag ccgagcagcg cgttgtcgag caccggcatc
     1741 tcgatcacga gcccctgcat ccggtccggc tcgcgcaccg cggcctccag cgtcacgttg
     1801 gcgccgagcg aggtgccgcc gaccaccgcg cgctcgatct cgaggtggtc gagcagcgcg
     1861 atcgcctgct ccccgaagat cgacatggag tagcgccaca tgtcccgcgg ctggtcggac
     1921 tcgccatgcc ccagaaggtc gagcgttatc accctgtgcc cgcgctccgc gagcgcctgg
     1981 gcgagcgggc gatgcatcag ccgcggcagc atcaggcccg ggaggaggag gaaaggacgc
     2041 gggccgctcc cgtactcgtc gtagacgagc cggtgcccgt cgtgcacgaa ctcgcctgta
     2101 cgcacgctca ccacggcagc ggcagcataa ctacccaacc tggggacacc tcacgcgttg
     2161 gagcggacgg gtatccgagg ggcccgatgc agacaagtgt ttcaactggc catgagagcg
     2221 cgcataatcc atctatggaa gtctcttcgc tgccccaccc aggcagcggt ctcctcccgc
     2281 gatcgaggcg ggtgctcgcg gcgttcggcg acgaacggct cgtggaccag ctccggcgcg
     2341 gcaacgaggt cgccttcgag gtcctctacg accgccacca ccgggcgatc ctctcgttct
     2401 gccggcacat gctctcctcc cgcgacgagg ccgaggacgc cgtgcagcag accttcatct
     2461 cggcctacaa cgaccttgcg ggcagcgaca agcagctcaa ggtcaaggca tggctcttca
     2521 cgatcgcccg caaccgctgc ctgtcgatcc tccgcgcccg ccgcgagcag gccgccgagc
     2581 tcgacgacat ccccactgcc gggctctccc agcaggtaca ggagcgcgac gacctgcgcc
     2641 ggatgctcgc cgacctgcgc gagctgcccg aggagcagcg cgcggccctc gtgctctcgg
     2701 agctcggcga cctctcgcac gctgagatcg ccggcgtcgt ggggtgcgag accatgaagg
     2761 tgaagtcact cgtcttccag gcgcgctcct cgctgctcga gagccgccag gcgcgcgaca
     2821 tcccctgcca ggagatccgc gagcagctcg ccaccctgcg cggcggcgcg ctccggcgcg
     2881 gtccgatccg caagcacctc aacgcctgcc cgggctgccg cgagttccgc gaggacgtgc
     2941 gccgccagcg cgccgcgctc gccgcactgc tcccggtcgt gccgagcgcg ggcctcaagg
     3001 gcggcgtcat gggagcgatc ggccttggcg gcggcggcgc ggcgggcggc ggtgcgctcg
     3061 gcctcggcgc cggtggcagc gccctcgcgg ggacgggcgc ggctgcgggc gggacggccg
     3121 gtctcggcgg catcgtctcg atcatcgccg gcagcggcgt cacgaaggtc gcggtggccg
     3181 cggtcctcgc gaccggcgcg gcggcgggcg gcgtcgcggt cgtgagcgac gcgggctcct
     3241 cctcctccgg cggcggtgac cacggctccg gcacgccgat ggcccaccgt cagcccggcg
     3301 acatcgccag gcccagcaag ttcagctcgg tcggcatcgc gactcccaag ggcgaggtcg
     3361 ccacggtcaa gcgtcacgat ccctccaagg agtcgcccgc ggccggcgtg ttcatcggga
     3421 acaacgccaa cccgacgccg ggcgagccgc gcgggaacgc ccgcggcaag gactcgaagg
     3481 acgacggcta ctacttcgtg gacggacccg ggcgctcggc ctcgcgcggc aactcgcacg
     3541 gcgcgaacgg cacgcgcgcc cgcgcgcgcg gcaacggcaa cgcgcgcggt aacggctacg
     3601 gctcccgccc ggtcccgccg cgctcctcgc gctcgcccaa cgcgggccag ccggccccgc
     3661 ccacgacgcc accgcccgca gccgctccca gcgggggcaa cggtgccgac aaggcgccca
     3721 aggagccgaa gggcgcgcgc ggcggcaagg gcccgaagct gccgaggctc ccgctcccgt
     3781 agcgggcgct agcccgccgc ggcgccggcg ggcggcggag cggccggccc ctcgtcgatc
     3841 ccgaaacgcg cgtgcaggcg gcgcagcggc cgcggcgccc accagttgcg gtggccgagc
     3901 agctgcatca gcgcgggcac gagcagcgcg cggatgatcg tggcgtcgac cagcacggcc
     3961 acggcggtgc ccaggccgag ctccttgatg aagatcatct gcgaggtcgc gaaggcgccg
     4021 atcgcaacgg cgaacagcag cgacgccgcg gtgacgattc gcccggtgcg ctggaggccg
     4081 atggcgaccg cgtcggagtc ggaggcaccc gcgtcgcgcg cctccttgat gcgggccagc
     4141 aggaacaccc cgtagtcggt cgataggccg aacgccgtcg cgaacaggac cagcggctgc
     4201 gtcgcgtcga gcgcgcccgg gctcgtgaac ccgagcagcc cctcgaagcg gccgtcctgg
     4261 aacacgagca cgaggaagcc gaacgccgcg ctcaccgtga gcaggttcat cgcgagcgac
     4321 ttgatcggca gcaccaccga gccggtcatc acgaacagga tcaggatcgt ggtgagcgcg
     4381 agcagcccga ccgcgtacgg gatgtggtcg ccgaggctgc tcttcaggtc gacgaagcgg
     4441 gcggcctgcc cggtcacccc gaactcgacc gggcccggga gcgaccgcac gtcgcgaacg
     4501 agatcctcgc tcgcgggcgc cgtgtagtgc gtgcgcgaga ccgcgtcgat ctgccacagc
     4561 cctggctcga ccggccgagg cgccgcgacc gatgcgatgc cgtcgaccga gcggatccgg
     4621 cgcgcgagcg ccgcggcctg cttcgggtcg cgggtctccg cggcgacgac gagcggcgcg
     4681 tcctggttgg cggggaagcg cacgttcagc tcgtcgaaga cctggcgcgg ctcggagctc
     4741 ttcggaagcg tctccggccc gaccgtcgtg aagctcgccc cgaggaatgg agtgccggcg
     4801 gcgagcagca cgatcgacgc cacgagcgcg atccgccccg gccggcgctg tacgaaccgc
     4861 gagagccggt accagccgcc gctgcggttg tcgcccgcct cggcctcggc cgcgcggcga
     4921 agccagcgcg gcgagagcga gttcacgcgc tcgcccagga gcgcgaggac cgccggcagc
     4981 accacgatcg cgacgaccgc ggcgatcagc gcaacggccg cgccgccgac ccccatggag
     5041 tagaggaagc gctgcgggaa cacgagcagc gaggcgagcg cggcggccac cgtgagcgag
     5101 ctgaacagga ccgtccgccc cgcgctgcgc accgtccgag ccagcgcctc cggccccggg
     5161 ccggacacgg cgatctcctc gcggtagcgc gagaccatga acaggctgta gtcgatcgcg
     5221 aggccgagtc ccagaccggt caccaggttc agcgcgaaga ccgacaggtc gacgccctcg
     5281 ctgaccacgc gcagcacgag gaacgtgagg acgatcgaca gtccgccgac cagcagcggg
     5341 agtagcgcgg cgacgaggct ccggaagaac cagaacgaga gcaggaacag gatcgggaaa
     5401 gcgagcatct ccgcgcgccg caggtcctcc tcaaccgtgg cgttcgcggc ccgggcggcc
     5461 agcgcgccac cgccgagcct cactccgcgc gcatcgccga gcgtccgctc gacacggtcg
     5521 gcgacgtcct cctccttcgc cgcggagcgc agggacgcga ccacgaacgc cgagcgtccg
     5581 tcgcgcgcga ccatcgactc cgagcctccg cgcccgaacg gcgagcgcac ggctccgatg
     5641 ccggggtcgg cggccagctt gcgcgcgacc tgcgcgaccc gcgcgcgccc cgcgtcggag
     5701 cggaccggcc cgcccggcgt cacgatcgct acgacgttgc cgtcggaggc gacgcccgtc
     5761 gcggcctcga ggcgctcggc cacctcgatg ctctcggtgc cggggtcctc cgcgctgaac
     5821 gggccgaggc ggtcggccac cgagccgcca gcggcgccgg cgaggacggc cgcgagcagc
     5881 gccaccacca cgacggtgcg cgcgcggcgc tgggcgagcc ctgcaatggc gtcgagcatg
     5941 tccctacctc ctcctcgggt cggccggccg tcccgcgcgc tcctcggcac gggcccacca
     6001 gtcggccatc cattcgttca actcgatccc gaagcggagc gtcatcatcg gccccgggtc
     6061 gggatcgtcc aggtcggggg cgagcgcacg caggcgctcg gccatctcgg cgctgcgccg
     6121 gcccgcctcg cgcatgtgct cgcgcagcgc gtcgcggtcg cgctcacctg agaagaagag
     6181 cttcagcagc gactcgtcgc gcagctccca gacgagctgg tcgcgcgagt cgagccacgc
     6241 ctcgagcgcg gcctcgccag ccggcgtgag tgtgtagacc gtgcgcgcgc ggccgcccgc
     6301 cgggtcgggc tcgccggtga tcagcccgcc ctcctcgagc cgcttcagct cggggtagat
     6361 ctggccgtag ctggcggtcc agaagaagcg cgtcgagatg tcgacgagct gcttgatctc
     6421 gtagcccgtc cgcttgccca gccggatcat cccgaggatc acgcgggcgg tgggagtgag
     6481 gtcgctcatg acccggcacg atatatcgtt ccgatacgtg ccaaccgcgc catccgtcac
     6541 acaagagggg tgtgacgaag gcgtcaccct gtgtttgtat aaaggcgcac acccctcttc
     6601 agaaagagca cgcacccgag atgaacaacc cgcctccgaa ctggtacccc gatccgcgcg
     6661 gcgaggccga gctccgctac tgggacggca gccagtggac cgagcacacc cactcgggcc
     6721 aggccgccgc cgcgccggcc tccccgccgc ccgcccaggc cccgccgcct caggcacccg
     6781 ctccggccgg cccccccgcc gcgggcaccc cgaccgccgc cggcggctac caccagcccg
     6841 cctaccagca gggcgccgcc gcgcccgcgg gcggcggctc cacgggcggc agcaagctcc
     6901 cgctgatcat cggcggcgcg ctggccgcgg tcgcactcgt cgtcgtgctg gtgctcgtgc
     6961 tcgccggtgg cggcggcgac gacggcggcg gcgaggaggc gaaggtcgag gacagggtca
     7021 aggaggccat caccggcaag gagccggagg gctgcgagga cttcctcacc accagcttcg
     7081 tccagaagtc gacgggcctc agcggctccg agggagtcga ggcgtgcaag aagacgcgcg
     7141 ggcagaacac ggtctccgac gccgaggtca aggacgtgaa ggtgaccggc gagaaggcca
     7201 ccgccaccgc gatccccggc agcggtccgc tcgaggacga ggagctcgag atctcgctgg
     7261 tcaagcaggg cgacgagtgg aagatcgacg acctcgaccg cccgtcggtg gcgcagggcg
     7321 acgacgccga gcgcgcgatc atcaacacgg tcctgaactt cggctcgtcc gagggcgtca
     7381 aggcgtgcga ctacctgagc tacgcggggc tccagcggct cggcgggcgc agcggctgcg
     7441 agagctcctt caaggagggc cagccagcga actactcgcc gcaggacatc tcggtcaccg
     7501 gcaccacggc gacgatggtg gtcgaggaga ggcgccagaa caagacgatc aagttcacgc
     7561 tctcgcgcga ggtcggaacg tggaagatcg actcgttcga gcagcagtag caccatgacc
     7621 gacgctcccc ccaactggta cccggacccg agcggcgcag cgcagctgcg ctactgggac
     7681 gggcgccagt ggaccgagta cacgcagggc gggccggcgg tggctcccgg ccagcagccc
     7741 gccccgccgc cgtccgcggc accgaccgcc gcgggcgcgg tgcccggcgc cggtccggcc
     7801 cccggcggcg gctcgacggg cggcagcaag ctgccgctga tcatcggcgg cgcgctggtg
     7861 gccgttgccc tcgtcgtggt gctggtgctc gtgctggccg gcggtggcgg cgaggagggc
     7921 ggcggcgagg aggcgaaggt cgaggagacg atcgagcagg tcgcgaccag caaggacccc
     7981 gccgtctgcg aggagaggca caccgtctcc tacctccaga aggcgacggg tctcgccggc
     8041 ggcgcggcgg tccagctctg cgaggagtcc acccccgaga acgacgtgac cggggtcgag
     8101 atcagcgacg tcgaggtgag cgacacgagc gcggaggcga aggcgcagat cgagggcggc
     8161 gactactccg gcgagacgct ggagatcagc ctggagaagc agggggacga ctggaaggtc
     8221 gacgacctcg accggccgaa ggtggccagc ggcgccgcgg ccgagaaggc ggtccagacc
     8281 acggtgctga acttcggctc ggccgagggc atcgccgcct gcgactacct gagctatccc
     8341 ggtctggagc gcctcggcgg ccgcagcggc tgcgagaagt cgttcaagga gggccagccg
     8401 gcgaactaca cgatccagag cgtgaccgtg agcggcacgt ccgcgaccac cgtggtcgag
     8461 gaggcccgtc agggcaagac gatcgagttc aagctctccc gcgagctggg ggagtgggag
     8521 atcgactcgt tcgcgcagcg gtagtcacca gcaccagcgc tccacctgcc gcgaaggcca
     8581 gcagcaccgc cagcggcttg gcgagcgagg tgccgtcgaa gtaggcgacg ttgcgcagtc
     8641 cggtggcggc cgcgcccgcc ggcaggagct ggccgccctc gcgccacggg tccggcagca
     8701 gctcgggtgc gctcgcggcg ccggaggcgg ggttggcgac catcaggaag atcaggaagc
     8761 tcagcatcgt gccggacgga ccgacggcac tcatgatccc ggccgaggtg aacgccatcg
     8821 ctgcaattgc gaggctcgcc accagcgtga gcgcggcgaa cgagccgggc agcgcgccga
     8881 tcgccacccg cacgatcaac atggcggtca gcccgccgag cagcgcgaac accgccagca
     8941 gcgcgatccg ctggggcggg cgcatggcgg gcgccatcgt gaacagcagc atcgcgccga
     9001 ggatcgaggt caccgagagc gggaccgcca gaaggttgac cgtggtgccg cg
//
LOCUS       DASVPL010000037        54832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_82734, whole genome shotgun sequence.
ACCESSION   DASVPL010000037 DASVPL010000000
VERSION     DASVPL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 54832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54832
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82734"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..13214
                     /locus_tag="VF517_02005"
     CDS             66..13214
                     /locus_tag="VF517_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX8051734.1"
                     /translation="MGSLTERLASPVQDCPSGNCTQTWDTTFSPAAGDQSRTPRRMRV
                     QATDQAGNTSSDRQRAPAPAVRWRLDDTVGNIAADSVPRAEQVTNTSFETDTSGWASV
                     GAFFLGNSTLTRIADSGAPHGGYVAKVVAPEWQRGIGTDVTVTAGVPYRVRVKVKRDP
                     GGQEIRIGLGEIAAPNEELVVASNATPSTTGYSDYTGVVVPNRSGTYRLYVRAANYGG
                     PPTTFYVDSASVKVDYGGTYAGGYTQDRPGATTDGDKAVSFDGTNGRVGTHYKPFVNG
                     TASTFEGWAKRTDTSGTHTLFGGNASTRAPILYVPEGSRDILWYSDVGTGQTRWPDAA
                     PTGAWFHWAYVFDEVADTAELFINGVSRGVRTVTDPYNATPGNLSFGAYAGGSNGFKG
                     SLDDVAVYPRALSATEIARLYSPDPSDARVAYWRLNESTGTTAADSAPHHQHVSNTSF
                     ESDTSGWASTGAFFLGSSTLTRVADSEAPDGDYVAKVEAPEWQRGIGTDMLLTAGRRY
                     RVRVKLKREPGSQQIRVGLGEIASPNEELVIASNAVPGSAGYTEYTGVATPSRTGTYR
                     LYVRAANAGGPPSTFYVDAATVTEDNAGTYTGGYTLNEPGATADADRAVRLNGSSGYV
                     AVPDAQTLDLGDRLSIEAWFKRSSLTGDQILVGKGQDAYVLMIAGGKLTLRKGGAGNI
                     VQSTATPGVDGQWHHVVATKDGPAAKIYLDGVDVSGAVSTLTLANNSAPLNIGTSAAA
                     DPEKWNGWVDDVALYSAALSGADVARLQKQHVYDWRVVFDNGAPVLDAPVRTPSGTAW
                     VNGTTQMQATLAASDPVSGIKRFKLFTAKQGGGEDLQTKSYDGCSENVDNLCDTSERH
                     TFTYTAGNMPEGSAQRVRAVAEDTVANVSAERSWDVKVDRTGANVGPVTGPLSTTGRW
                     VRPGVNYSFESGASDSLSGVESIQFFVNEQLQATQPGDNCTPQGCASNPSPKTFSWTV
                     PDPYTGQGDPSLRVKANDLAGNPGSSRTWNVRVDDDKPAVPTVTGTLKDAEGKFVNGA
                     SFTVHVDASDGTSQSPSSGVKQIRLLVGADPSSLTLRKESPVQTCATQNCTQPWDTTF
                     SPLSTDETRAGRLIRVEVEDQMGHVEKRDWYAGFDRTAPSAASPTHADPEDWWYSETA
                     QRTATVNATDSTSGVASVRLSVPQLGGGPSVQDHPFGCSNVVPATCATSGSHPFGYGL
                     ANVPEGQVSVTGRATDVAGNNSWDVTRTVKVDKSAPQFVSKSGTASVDGLWLDDGEHD
                     VTVDWNDAFSGVSSSRVAFQAPSLALDGFERTALAGWGEADAGGPWSAIEGPASDFSV
                     AAGRASVSSPQGSPRTAVLGSTPARAFMARVKVRLPDVPPASGWSEAAIVNSHSSGSF
                     HVRAALELYPDGHLELLFRDKANNELARTDLGMSHVAGDDYYLTFQKPGGMSIRAKAW
                     KVGTPEPAFPQVLISDPYVYSGGSRLGLRAINGAAVTQSTAFDDLDVQPLRSGDYEEP
                     FKSGGCDRTGCAGPMSHSFTWSTAGEPQGLHHVSPTAADPVGHQTQGTQWAVGLDRSG
                     PALALSGALKDADGQTVYREDMALHMDARDGRAGNDATRRSGTERLQVWVDRAGPQDP
                     ADHDQSQACTPDSCAMEGDYTFSPTEHGPGAHTITVRATDKVGNQTEQTVTVTVAADE
                     AGPELELSGDLRPPLATGRSVHIKALEERPADTGVRKIELWLDPDLTPTSDEREHGHE
                     PDCSAGCPSVAETDWALDQATPPGKHEILIVATDDAGHRTEERYEIHVLDLLPSSRSK
                     LGLEHWFDYDDTAAGGDSSVYVNGETGNTVWHSVPVVNPGRGLSTVVNLTYNSHDRGG
                     VLGSTLGRVPLVDLSGQDLNNDLPGLSYSEAGVGFSLGISGPTRLNEPLGGVLIAQAV
                     EEGEAAIPGFTGTLPGESNLVVTMTDSDGTVHTFTKGADGEWIAPPGVSMRLRRFQNG
                     GTLVNPIDEKWALTRPDGITHFFDNLGYLTKTKDRNDNVLEYIYEPYDALTGNVTKSV
                     VTSSGQNLDYALCDGVQLGKQTEVTLPDGTEVPVFCAKRVSRVKDPAGRELKIDYNDY
                     DSSYLGLADGKLPIEFRADFPGLIGGRAGRINKITDHANREYTFEYDDHGYLTKFVEA
                     ANRPEKRTTELAYEDWKPGLEAIGQDRQLIEVKEGETGRKTLIRHVHPDDRTTLPTPG
                     VLLLPRQACGVTKRNDGVGDLESVPVANQECRTADNDLEKTYDYTAPEGSTPRKFEVD
                     EILMRAQAVGESATVGTTVHEIDDEGRPEAVTDPNGVKTALTWNEDVNAVSSIEEASG
                     TDDASRTELDYDQANRTAVIAQQRKYPNFPSTDGARVTNFSYQFSNGRHHSTASGVSD
                     ADGQFVADMTEVRNPKTGTGQSFEIEQVAGAYTGNVTKRWDKPGKSGNVATTLYDGHG
                     QIISEDDEVAENGPTIYEQHDPTGQPQKVLDPSGKRWDYLYDAVGNLTTVVDPRATDR
                     SGAAGLPYTTTLGYDALDRLVNERIPRLSADSDLPDSKRFTLRSREFNRNGTLKTATD
                     GEGTTTSMTYTPMDQPLRVTAPGKAGRPETTDHVYDDADRLIARVEPKGTGATVATAG
                     EHQKACTDASVDPPVVDHMTRYCLDRGGQMIAEVRAATVLPPNGGPSALIKSFAYDRR
                     GNVTGIVDPLRNSGRSIREATDAAQVSDPLTTSRRLTYEYDRVDERTAQIEHVTETGP
                     DGRLPAKRTAYEYDANGNKLTVLPPKAFVGRSASDPDRDYQTKTSYDHRDLEVAVRTP
                     AGCSAYQRREDGRITAITAPRGTTATDADDCSAAGPFKYHTTKLTYDAQGNLASRTIP
                     FAQGQYGRADSEFENWKVTYERDDVGNATSIEDPRGNEFDNTFFDGGQLRSTTRPSWY
                     ELEWSDDHAPRDPAQRYKQEKDAGGDFELADGGPEIRESEIMSRNAQPEGERSDLPET
                     HGQGDFGKVDPQDPGDLLPEAGATNLDYDREMRLTTISDAAGGDRTISYDEAGRVIGK
                     SWPFKPDDPITHDFEYDLNGNLERYTDGRGKPTTYGYDGFDRRISETTRGAGRAPGDA
                     FTEEVTRFAYDANDNLEWRQTPRGTALRFDFAYDSTDHLISEANPANDTWEYDYDVNG
                     NRVKERSPRYQSATAGDKPDFERTFTYDPANRMTEDDPGLGDGFTTYEYDADGNVTEM
                     TSGGAADANGAASARRKQTSSYDGRRLAWRRTTSTAGGDDKRTQIQEYDANGNLRRMI
                     QGKGVEDHSRAKVLDAPGTSTADVTWHATLRDYDEDDQLVAVHSPWSRPPSEGSPAVD
                     DPDGPSDAETGGDDRKIVQDFLRPTAGNPLRRVTSIVAPHESSDTVAARTSYTYFANG
                     WVKTQSDEKLVRPGSTAPVRTANVTYDYDKAGNQTVWKTDEAGDSPDGRLVKRFFYDD
                     GTLKRREAYKTKAGTDDETRRTYDYFYNENRSLVRIDDFDATAGGTGRARSTKIKRDE
                     AERESVVNEDWDGGRDTVLEYDRAGNVTTRKTDGIYVNPTTYNGDERKVTTFDYDALD
                     RETSAVVDPAEGDTRTTTTTWWDSNDMKTRTKSNGTVESYFYNARGEMAQKRRNPDEG
                     DTETQDYDYDRDGNRTQDERGTHVFNPRGQLVKWTRGPKYSHSEQAKDKDGTVVTYER
                     NAGGDVTKEVDNWKPTGITGDTTTTYRYHGERLIWTETSRPTVSGTQTTHSDYSYDDF
                     GSVTKVVHEQTGPGSDPNEPPDGPPSSVSCSEMPNEAEAKVTRYCYDEFERRVQSRGS
                     GIEKPTIYVYDGLDRRDRKTVEENGDPKVHEYAYVGLNDLLSRETDKDGKKKTYDYDS
                     DGRRLGQSVKASGDSAPTHRPYATDASGSVEGLEDTEGRFGELNRDTYLYDPYGESEN
                     VGGLNETEDPGLSDAAKENPFRFQGFYFDSGVKTYDMQARDYRPDIGRFLSEDRYEAA
                     VGDKLLQADPLTQNRYAFAGGNPVNNVEFDGHHIMRCYSADHCSGAYEHWRWHRRQAQ
                     IQKQRRKDTIVKNAQNNARQALAQGDVKRYYRNLAVIIEQDQAKLNRRVQALQAAVGP
                     LEAAQPKKPGFWDYAKVVFDPTDPLDILLTAASAIPTGATQGAKGAKIALKGAKLAAR
                     VAKAGKVGKALSKVRTLLAGAVSRAGDNASLVNRLRQCGNSFTARTPVVMADGSRKPI
                     ARVRLGDYVVATDSATGRTVAARVNGLLRSSAGKDLTVITVGGRRITATDHHPVFVLQ
                     KGRWVDAADLEVGDLLLGADGEPVPVSAVRDVDADVPVYNLTVNGIHTYYAGDRPVLV
                     HNSGSCDVDAALEAAAAELKQLNRGKNRVTINTPGGRMVVDLQGKAHYSKAQGRNVPT
                     PHVKMQTRNQNPLDPNQFTFSDSDVFSAGWQEIRIVRQALSRRAGN"
     gene            13211..13612
                     /locus_tag="VF517_02010"
     CDS             13211..13612
                     /locus_tag="VF517_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051735.1"
                     /translation="MRFVGLREWPMNEPVIECETESHCYDLHNAGDFRGVEWTPPGAL
                     AFRFVYDPDHLHTPGEGDLRTALELRFTGVRSLHTVLAPDFAVESSALVEHWVWNRVS
                     DELGGFEFKVGELTLNFRATAVELITAPEPL"
     gene            13676..14989
                     /locus_tag="VF517_02015"
     CDS             13676..14989
                     /locus_tag="VF517_02015"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX8051736.1"
                     /translation="MDWTEAHVRRLFWRAGFGATPQEAARFAQAGMDATLRFVLRGDG
                     GAELEGPEPSADGQPLDPVNEWGHDVLWWLDRMVRSRRPLVEKMTLFWHDHFATSGQD
                     TPLMLRQNKMLRKHALGDFRALLKDVTRDHAMQLFLSLAGSTKWAPNENYARELMELF
                     TLGSGYTERDIREAARALTGFEARWTDDGFKGIRYRKDWHDTGQKRILGRRGRFDWRD
                     VCDIVCEHRRHAPFLVEKLWAFFVTEPLDPGTRRSLVKTYTRGKLRIEPVVAQILAHP
                     ALYARLDRPDMVKAPIVYLAGALRATGAGVQRESWVWLSEQMGQQLFHPPSVAGWDWG
                     PAWLSTNTMKARFQIVTYLMTDDGPLEVPDKSVAIGVPVGDALESARAATGRPWTSPE
                     TDAALDRLATTMVAGLKSWERQQRADQRERALRHLLLSGPDAQLH"
     gene            14989..16344
                     /locus_tag="VF517_02020"
     CDS             14989..16344
                     /locus_tag="VF517_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX8051737.1"
                     /translation="MPKSTHRACDDFHATSEATRRSFLDSGTTRRQILARGIGAGLAV
                     YASRAMSIADVFGAAEASAASGPEAPILVSVFLPGGCDLLDSFVPLEQYGRYADLRPK
                     LKQKDPAALGSTGLGVHPSLASGAGGGIKGLFEQGKIGLLPGIDYANPDLSHFHSRHF
                     WETGLITQTAGTGWLGRMLDRSGSADNPLQGISLSGTLSPLLRTAGAPVAAVHSPNAA
                     QFWIPGMWGSSQEIAMQAWSEMAERQGGGPGERAATTSARLAKQVADRLAPYRHDDDE
                     PNPLASPVEYPKDNDLAERLSQLGGLLSQPLGIRVAAVEAEGEFDTHDGQPADLARGL
                     KDVSEALAAFQADLEARGLADRVLTFVWSEFGRRPQENESSGTDHGAGGLAWVQGTRA
                     RSGVLSDYPDLGRLDRQDNLAVTVDFRRVYCSLLEQWMGADAGAVIPNAAGFGRMELV
                     S"
     gene            16341..16745
                     /locus_tag="VF517_02025"
     CDS             16341..16745
                     /locus_tag="VF517_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051738.1"
                     /translation="MTRRRAIAGALAAAAALAAPAASGAAGSGDGPLDVRGTAVGVSE
                     REFRISPYRRTVPTGTLKFNVRNFGEDVHDLVLVSPRGRKLGSTGAIAAGEQGVLRIK
                     IKTPGVYRLVCTQADHAARGMKSRIAVRATRR"
     gene            16952..26968
                     /locus_tag="VF517_02030"
     CDS             16952..26968
                     /locus_tag="VF517_02030"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HEX8051739.1"
                     /translation="MLLALASSASAAVTQKQAAKKALAALGSEKRSDAVVVFGLPTPL
                     KAGTKVTQAGTAKSTKGSTKGISSRLRSAGVRMVKAPAVLTVTKRSYFFYEDRGPFEL
                     YQHPGRVALVDAQTGAVRLSRTITWPPLLNGRLPAFLKSAGGYRSAKYQVLYRPYAID
                     GSAQQPRTANRNLFDQDPFGPGRPLPDMANSKAIADRLAAEHSCILRVTDTLPSFWNV
                     NQLNLTRTYVGTLFEKLERDNGGFIDDRYGARSGETLAQSVDRLVAAGCKDMLLYVAG
                     QGYDQGGDPVIQLGTSVMKGGLLAQQNLSASELRKLVSGRPGVTFKFKFDAPYSGGFM
                     SRLRDLSNVLLIETPAAEGDTAFSFIPEVAQNGQMVSAPSNPAGLLEFTNCELNGFKA
                     FFSSKEEIDRATQAQNEGVSFLAEMLARSQELCKGDGFADDLGSNPQLYAAKRGSGKG
                     TNLPPKADPQSVAATEDTPKAITLTGSDPDGDPLTFTITKQPDHGTLSGSGANVTYTP
                     AANYNGPDDFSFKVTDSKGAESNTAKVSIDVGAGNDAPVVTNSQNATPYTEQAAAVVV
                     DGNLTVSDVDDTQLEGATVRVSSGFQAGDKLNFTNTSAITGSYNAGTGVLTLTGSDTV
                     ANYQAALRSIKYENQTENNPSSPKTIEFKVNDGTATSAASTKNIAVTGVNDNPVLDTT
                     DAALAYSENSGAQAVDNGITVADSDSATLAGATVQITGNFAQPEDDLVFTNQNGISGS
                     YNDATGTLTLSGTASVANYQAALRSVKYQNTSDNPSTATRTVSFQVNDGAGANNLSNV
                     ATRDISVSTANDAPVVTTSAGNTSYTEGSPATTVDGSLTITDADDTNIEGGQVRISSG
                     FQSGDDLVFVNTANISGVYNTGTGVLTLSGTDTVANYQAALRSVKYRHTGDNPATSKT
                     VEFKVNDGDADSNLATKGLAITPVNDAPTVNTTATALTYTEGSGPQPADSGLGLSDPD
                     STQIQGATVQITGNFVSADDQLSFTTQNGITGSYNDTNGTLTLSGTSTVANYQAALRS
                     VAYENVNDNPSGTKTVSFQATDAEGAASNVGTRDINFTAANDAPVVDNSAGATSYTEG
                     DPGTTVDAAVTVSDADDTNLEGGQVRISTGFQSGDDLVFVNQNGISGVYNTGTGVLTL
                     TGTASVANYQTALRSIQYRHTGDNPTGGKTVEFKVNDGDVDSATDTKTINITGVNDSA
                     VLTTTAAALSYSEGSGAVPVDSGLTLDDPENHDITGATVKITGNFAQAEDDLVFVDAN
                     GITGSYDDATGTLTLSGTASEATYQTALRSVAYQNTSDNPSTTTRTVSFKTTGDTGSP
                     SQGSNTATRDINVSPANDAPVVTPTNGSTAYSEGGAATTVDGGLTVSDADDTNIEGGQ
                     VRISSGFQSGDDLVFVNQNGISGVYNTGTGVLTLTGTASVANYQTALRSIQFQSTNDA
                     PVGSKTIEFKVNDGDVDSNAPTKEITVTPVNDAPTLNTSGGSASFVEDGSGAVAVDPN
                     ITLTDPDSANMSGATVSITGNFDSSEDQLLFANTANITGNYNSGTGVLTLTGTDTVAN
                     YQAALRTVQYNNTDTVAPNTATRTVSFQATDSSAAASNTATRNVTVAQSNDAPVVNTT
                     GGDTAYTEGGAATAIDSGITVTDPEDTNLEGGQVRISSGFQSGDDLVFVNQNGISGVY
                     NTGTGVLTLSGTSSIANYQTALRSIQFQSTNDAPATSKTVEFKVNDGDVDSNLATKGL
                     AITPVNDAPTLNTSGGSAAFVEDSSGAVNVDPNLTLTDPDSANMSGATVEITTGFDSS
                     EDQLVFVDQAGITGAYNSGTGVLTLSGTASVADYQAALRSVKYNNTDTVAPDTTTRTV
                     RFQATDSSAATSNFATRNVTIASVNDAPVVATSAGSTPYTEDDPATTIDGSLTVTDQD
                     DTNIEGGQVRISSGFQTGDDLVFVNQSGISGVYNTGTGVLTLTGTSSVANYQAALRSI
                     KFRSTNQQPVTSKTVEFKVNDGDVDSNLATKELAVTPTNDGPIVDATNTTLSYTENDP
                     ATAIDSGLTIDDLENDNLSGGSASITANYQAGQDVLAWTDNNLADNITLDGVNSNAQT
                     VVLTGLDTEANYEAALRAVTYQNVSDNPITANRTVTFSATDQPGLTGSDTRTISVTAV
                     DDPPVAVNDSATVLEDAAATSVPVLTNDTDPDGGPKTISSATDPANGTVVLTGGSPGA
                     HTGLTYQPDPNYCNDPSGSPDTFSYTVNGGSNATVSMTVTCVNDAPVANNDTFGGAGN
                     VNDQAIGNTTMQVDDTNDGGDDNKPAPTNPHTEIQGDILANDTDVDGPTPRIVQSAGS
                     DAGATNGQTADGGTVTIEPDGDFVYQPPASVSCDNGTDTFNYKISDQQSSGAGPIPGT
                     AIGTVTINLEGCVWYVNNNAPGNAGTSTQPFDTLAQAETASGANHTVFVFDGDNTSTG
                     YQTGYQMNSGERLIGEHEGLVVDADQGGSMSADTLHPANPGAHPTLSGTNEDVVALDD
                     GNEVRGFNLDPVGTGSGIAGASGDTGGGTIDDVNILDTAVAGSEPLLELDSTTGTFNI
                     SNLVVNNQAAVSPPNTATGVRLNNAGTVNFAPSSQISIGINGAKGLDAASTGLGTSTF
                     DDITVAGSGVGGVRMDTTTGTVALGDGTGSDLSLTTTSGSEAALRVNSHTGGALTVDS
                     AGADDLHATGGPALDTTATSGATYSFDDVDSTNSANDGINIDGLGTGTFSATSGDIGG
                     YAGIGFDLNAGSGAITYPGTFANGSGTYVSEITGRSGGVVSLSGSISDTNDSGGGINV
                     TGNTGGSTVFSGATKQFNTGTVDGVDFQSSDGHTLVLSGGGTDIDTTSGRGVDAETSG
                     TIQISGSGNTVLTDTGRAINVANTDFAAADMTFDSVRVNSDLAGSGPDIGIRLDTTGN
                     AGNLAVTGTGGDCTSTASCTGGVIHNTTSDGVRLTSTAGTSLTDMYIGDSDGTGIYAP
                     SGTSNNTFNDLHIHNNGDAVSAPGVPEAGIAFGSITGTNSITGPNSSAGTTTVEASRN
                     NQIEWLQASGTGTLNISNTDVINGGQGAGTGSNGLSVESQGTATATLNVGNGGASADS
                     LIDNNPAAAMVTNATGAGATINSDVDNVDFDGPGSFGLSHLGRGTTVWRVNNSDFNGF
                     NVAPNNQSAASTVTINPIESGSMDGTFTNNRIGTAGVNGSGALRNFGLVLGIEDGSDA
                     DLNISNNTIRETAQEGIFLYARDFLLQGGSVSNVNLTLRDNVVSEIENLPSPDTFTNL
                     NGIEVEAAFDSNVCADIANNDSESVGSSSTTGTELRTRQLDDSIFRLEGLPADSGNNE
                     PLVESFLASRNPLIPVTEIDAGIEQDATPEGYKAQPGDCPMPITPP"
     gene            27005..28087
                     /locus_tag="VF517_02035"
     CDS             27005..28087
                     /locus_tag="VF517_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HEX8051740.1"
                     /translation="MTVQAPSGLEGVVAFQTQIAEPDKEGGALRYRGVDIEDLVGSVP
                     FAKVWGLLADDSYEPGLPPAVPYPIPLRTGDTRVDVQAGLAQIAPAWGFQQLLDISDE
                     EARDQLARASVMALSFVAQSARGMGQPPVPERDVDQARSIPERFLIRWRGEADPNHVK
                     AIDSYWISAAEHGMNASTFTARIVASTGADAAAALSAAVGALSGPLHGGAPSRVLQML
                     DEVEASGDAEGWVRDALDKHERLMGFGHRVYRAEDPRARVLRRTASEIGAPRLEVAEK
                     LEAAALAQLAERYPDRVLATNVEFWSAVVLDYAGVPPDLFTPMFACARVAGWSAHILE
                     QKREGRLIRPTAEYVGHAPRSPDEVG"
     gene            complement(28281..29099)
                     /locus_tag="VF517_02040"
     CDS             complement(28281..29099)
                     /locus_tag="VF517_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051741.1"
                     /translation="MADANRSEAPRAEAAPPEAARPEAARPEAARPETARAEAPTLSV
                     AGTWKRLNFDQRAAAVGAVLLIVSTLGPFSFVEAAIVLIGAGVLFLLRKRAEGKLFHV
                     PFGDGAVIAAAGAWAALLIVVRLLDRPPGQSLLALVCAAILLLAGLRAHANRPPDDLP
                     AGAAEPGPPAAPPPDPDDREPPPAPPRAGEPGPAPPGPPASDPPGPRELAPSTPPRPA
                     PGGEQPTAETQALPARVLPPATPRGARRPPGAPAEPAPPQPPLWDEPDADSSQS"
     gene            complement(29077..29451)
                     /locus_tag="VF517_02045"
     CDS             complement(29077..29451)
                     /locus_tag="VF517_02045"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX8051742.1"
                     /translation="MATRHYTVAEANEALPWVLERIERLRSARVRLTDEEVREALAEA
                     APMNGGGEPGRHVSEAFLDMRSALGELQASEIVLRDLDRGLIDFPAQRDGREVYLCWE
                     EGEDEVGYWHDLDSGYGGREPL"
     gene            complement(29451..29909)
                     /locus_tag="VF517_02050"
     CDS             complement(29451..29909)
                     /locus_tag="VF517_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX8051743.1"
                     /translation="MKTWILTGSPENFAATRERGFKLIGMKQIRRRMAEQVEPGDRIV
                     FYLTRIKEFAGIVRVTGEMFEDRTPVWPGKPGKVDAYPWRFETEPELVLEEHEYVPAE
                     ALADELEHVRKWPREHWTLAFQGQLRTVSDADARLLEERLRAASGSPAPS"
     gene            complement(29906..30130)
                     /locus_tag="VF517_02055"
     CDS             complement(29906..30130)
                     /locus_tag="VF517_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051744.1"
                     /translation="MEQQSSNGVPPQPDLSALIAVLEALRRAVPAELQEQLTTLQREL
                     LLTVRALIDWYLERLDKPELAPQVEDIPID"
     gene            complement(30111..31307)
                     /locus_tag="VF517_02060"
     CDS             complement(30111..31307)
                     /locus_tag="VF517_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEX8051745.1"
                     /translation="MPPRTILYTGKGGVGKTSVAAATARRCAAAGLRTVIVSTDPAHS
                     LSDSLETELGGSPTPCGDGLWGQEVQAQEEMERHWHAVQDWMGQMLTEHGVDRITADE
                     LTVPPGMDELFSLLQIKAHHESDEFDAVIVDCAPTGETLRLLSFPDVASWWLEKVFPI
                     ERRLMAAARPFARTLLDVNLPPERVFDDVERLVTNLGSMNGILRDRSTTSIRLVMNPD
                     RMVIREAMRTYTYLNLYGYLTDAVVVNRVFPEEAGEGYFAGWREVQQEQMELVRSAFA
                     PVPILTAPYLEQEVVGGAMLDRLGDEVFDGDERAEAILHQDLSQELVSENGSATLRLA
                     VPFAAKGDIDLKKIGLEVVVRVGGQKRTIMLPATMATYRPRSARFEDGALSVLFEKEE
                     HGAAVE"
     gene            complement(31329..32849)
                     /locus_tag="VF517_02065"
     CDS             complement(31329..32849)
                     /locus_tag="VF517_02065"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HEX8051746.1"
                     /translation="MTAVRFTCTLCLLALALAAPASASPVLHWDGDRLEQSDDPALPP
                     PALSDPPAPPGPCAQPALRASATSVNQAIDRASSRKQIDAPAAAEYKRIYREARATRA
                     KLSGSYRSELSSVISQLERIAAADRLTGSRMPALFLQLDRNDEFWGGDPEFPAREDVP
                     EEPCQPASSGRAGSRIMFSGSEIVYQYYPGNGLQIQPLANFGKANGIYTACRRKEARC
                     DPVALRKLLDELVAIRSSRGGFTTWEYWFAFGGGSPPWVSGMAQGTAMQALARGSILL
                     AEPSYLRVAKSAVGIFEKSTPTGVRVRGEGGGSHYVLYSFSPGLRVLNAFLQTITGIY
                     DYAKTAKDDAAMRLFRAGDRSARREVPRYDTGAWSLYSQGGAESNLNYHNVVTDFLEN
                     LCTRTKASVYCQKAERFNAYKKTAPKVAYAGPSSARTGRRTALTFTLNKVSCVTATIK
                     DAAGAQVYRSQIKFNRGRRSFTWQPQTAGKYTLEIDTLDLRKNRTVLTRTITVRSG"
     gene            32896..33903
                     /locus_tag="VF517_02070"
     CDS             32896..33903
                     /locus_tag="VF517_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051747.1"
                     /translation="MAALLAGCNGGGSEGDAREILQKGFGGSIGTARLTVDLTAKLEG
                     IPQQKGPVRIELNGPYRSGPPGRLPDADLDMAISGGGQTFSLGLISADERAFVEFGGR
                     AYEVSAATVARLNGGDSPAPGGGPGLRGALRQRFGVDPLDWIADPRDEGEATVGGDAT
                     RHVRGDVDVKKALGDLNRVVARTAAPGSQPPTLDDAQVERVSELVDDPKLDVYVGRDD
                     GKLRRFSLDLAFEVPERDRRRLNGLRSGTVTLDVELNEVGQPQRVEAPTSSRPYKELN
                     DLLGGRGIIGLLFGATTGAPQPGGPNSPTQQQLEAYRECIDQAKPSDTATIERCNELL
                     R"
     gene            complement(33910..34893)
                     /locus_tag="VF517_02075"
     CDS             complement(33910..34893)
                     /locus_tag="VF517_02075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8051748.1"
                     /translation="MRQRNFGTTDLTASEIAFGTWALGSTWWGETDDADRLIGRALEL
                     GVTFFDTGNVYGQGKNEEMVGRALAGADRDSIQISTKFGYVLDSGRQEHSEGERPHDW
                     SPAHVRESLEGSLRRLGTDYVDLYQLHNPRMDAIENDELFAVLDALRDEGKIRHYGIA
                     LGPAIGWRDEGVLALERRGIAALQTVYNLLEQDPGADFLAAAERSGAGIMARVPTSSG
                     LLEDKYTLDTEFGPGDHRRHRPRQWLVDGLQKIDRLRFLVIEGERSMTQAALKFILAR
                     PQMACVIPTIGTLAELEEFAAAADAPDLTDDELARVQELYVRNFDVEPAAA"
     gene            34947..35759
                     /locus_tag="VF517_02080"
     CDS             34947..35759
                     /locus_tag="VF517_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051749.1"
                     /translation="MGKDKLSGRRVVITGASSGIGLAAVPRFAAAGARLALIARDEER
                     LAEAVDAAGPAARAFSADLGEGDSADAAIAAAARWLGGIDVLVLNAAESSYGPFRDTP
                     AEDFERTIESTFGTGVNAVRAALPHLDESEGSVVANLSVLSRFPVPMFSGYVAAKHAL
                     RGFLGALRIELERDGSGVQVSIVHPGHVDTPFWGRVNSVTGRLPRLPPLAYDADRVAA
                     ALVDAAERPRREHTVGALARLQVVVAAVAMPLVDLSGRALGRWLESGEQAAA"
     gene            35771..36778
                     /locus_tag="VF517_02085"
     CDS             35771..36778
                     /locus_tag="VF517_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX8051750.1"
                     /translation="MVADIESIAILVAALLVFLWLDLHFFARGREPTFREGAIWSIGW
                     LVVSLLAGFVVAAVRDSDDAVLYTTVYLIERSLSLDNLFVFILLFSYFAVPQEQRGRL
                     LFWGISFALVLRGLAILGGVALIEQFHFVIYVLGVGLLVLAYRILRGVAENVDPDKNL
                     MVRVVRRFFPVTGEFHGRHWFARVQDKRHVTPLFLCLVAMVFADIAFAIDSIPAAFAI
                     TTDGFLIWMGNVFALLGLRALFVLVQALMKRFRYLDETIAIVLALVGLKLLIEDIVKI
                     SPEASLAGVALAFAAGISASLIADKRDPEGAPPERGEPLLDSTASGVEEDESEDRVQS
                     E"
     gene            complement(36731..37816)
                     /gene="cax"
                     /locus_tag="VF517_02090"
     CDS             complement(36731..37816)
                     /gene="cax"
                     /locus_tag="VF517_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX8051751.1"
                     /translation="MRRPVFLLAAFIPVAIALEIAHANPVLIFSAAALGVIPTAALMG
                     MATEELAAKSGPGIGGLLNVTFGNAPELIIAFFALLEGLQEVVKASIVGSVVGNVLLV
                     LGAAMLVGGWNREKQTFNATAAHAQSAMLLLALAALILPAVWQLIHGGGLPAVGEERV
                     DFGTDLEHLSFGVAIVLILTYVAGLVFSLKTHRELFNPYTEGEHEDTLGWSVRKSLGM
                     LAVAAVAVGVMSEILVGSISDASEDIGLSQFFVGVFVVAIVGNAAEHWVAVLVAAKNK
                     MDLAVNIAIGSSAQIALFVAPLLVLVSFVVGENPMALVFNGYELGALVLAVLIANFVT
                     QDGESNWFEGVQLLALYAVFGLVFFYA"
     gene            complement(37847..38188)
                     /locus_tag="VF517_02095"
     CDS             complement(37847..38188)
                     /locus_tag="VF517_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051752.1"
                     /translation="MASDRQFELDELIVRPGTYFNPQTEVLLVVDDSASLDNEIFNLE
                     DFEGADWVLISDDLPVDEARRDEMVEGFQARQAGGDGRDVTLDDEDEQERLDAVEDDE
                     DPKPEAAEEIE"
     gene            complement(38239..38685)
                     /locus_tag="VF517_02100"
     CDS             complement(38239..38685)
                     /locus_tag="VF517_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051753.1"
                     /translation="MIVHTVGVESAFETVLWVIVILATLAAFAAFVGAGGVYDRIGKG
                     DFALDQPDRTPGPAPGSAAADAEAAAEIRQMVEAKSARREARGEDALDIDAEIAALTR
                     PAGADAALRDEVRQLVVARNERRQRRGQEPLDVEAEVDRQLRDIGA"
     gene            38684..38821
                     /locus_tag="VF517_02105"
     CDS             38684..38821
                     /locus_tag="VF517_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051754.1"
                     /translation="MWRRLRRRTMAPSRPAIYPDQIGLVILTVVAIGLGIYLMVRVLA
                     G"
     gene            38915..40090
                     /locus_tag="VF517_02110"
     CDS             38915..40090
                     /locus_tag="VF517_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8051755.1"
                     /translation="MVDLTLTDEQKDLREMAHNFAANEIRKVAWEYDKEGTWPQDIID
                     KAWELGLMNSHLPEEYGGAGLDFMSGTLIEEELGWGCSGIGTSLTCNGLATTPIMLGG
                     SEETKKKYLGMLSEEPKLASFCLTEPDAGSDVSGMRTTAVKMGDEYVINGSKCFITNG
                     SYANYYTVYAKTDKDAGHKGISAFVVDRDLDGVTVDKKEDKLGQRASNTATISFNDVR
                     IPAENLLGEENRGFKLAMMTLDRTRPGVAAMAVGIARAAFEFATEYSKERVQFGVPIA
                     MHQAIQFMIADMATKVEAARLLTWQSASLLDQGKRNTLASSHAKRFAADSAMEVTTDA
                     VQVYGGYGFIKEYPVEKLMRDAKIMQLYEGTSQIQRLVIAREVLLPRQVSEPAAATA"
     gene            40204..40731
                     /locus_tag="VF517_02115"
     CDS             40204..40731
                     /locus_tag="VF517_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051756.1"
                     /translation="MKRLVLFAVVGALLVAPAADAKEYLFVGSDGTRCTVSTAHSTAP
                     EAGGTFSADISSTASCSRPLFGAFIRAYVGSADDPFGYEAGGFSDSCNPLYPYGTGCD
                     RAVVTAGGTRGGLAPGSYVHGTLVTLTLAQAPPPSWVVVPVHEDRPESISFVGTCRPV
                     YADTRCHFREPFTAG"
     gene            40915..41898
                     /locus_tag="VF517_02120"
     CDS             40915..41898
                     /locus_tag="VF517_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051757.1"
                     /translation="MASYDQVKAALTLMHDELSAKSSAVLAVGVGFADENATRREPGT
                     DCVIVVILDENGDDRAEAAFPSSVTLTADKLRSAAADDEIDGDVEIPVRIEREDIQPL
                     IVKEQGEPVVEPLDDPAARGAGAFLLGGDPIYTSAGDWIGTGGCVLRIYNTLCLVSNA
                     HVLSEMGGVGGRVRDHRGRVDFATVNELRTAPDSAWAQLDSDAGYRNDIPYIGQIRWV
                     GGPMQLGWHGRKYGSSTGWSDFHFRFEGTTAGGTPAVGGWWEGQPHGGKPTVGNGDSG
                     SVVVGDGGEVMGLVFAGSEKVGTSPGGSSFRKIWCVSNMWTAFPGPEVPAW"
     gene            41895..42917
                     /locus_tag="VF517_02125"
     CDS             41895..42917
                     /locus_tag="VF517_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051758.1"
                     /translation="MNAATGGSEENPPDPRLEIHPRIRTALRGMGHSIFPFNAFDEFG
                     ELTLTPLKIPAQYTELAKQVAQSPVFEAVYTHISVLEEEPDWRCYSLGLSFTPDYGIS
                     IIPVKSLDSLNVSREEAPRVAGEWATSLNELILENVQQVAGADYVLPDQIAKAVKELL
                     PIVRKLLPDKPGGGQGPGPGTEPGPGPGPEPEPGPEPGPEPIVPLDLLEAPGQHHAGV
                     PALEAADAALAAASPWSPVLGLELAAAVLSAAAMLRKKQSVTATIVWYPPDSWEGFDE
                     AEWDHYRKHLKPISYRGRRKVVHACWNPFSRKVLAFGPDDLWNSDNPHVPKRPSQVQR
                     AFYPIG"
     gene            complement(42927..43172)
                     /locus_tag="VF517_02130"
     CDS             complement(42927..43172)
                     /locus_tag="VF517_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051759.1"
                     /translation="MKSKGIEESMRSLGFLAFAVAVGAWVVGGAGGLDAGAVVAVVGV
                     LLSLGAFVRAVRRDELPGATMWAVAGLALDLALFAGV"
     gene            complement(43169..43399)
                     /locus_tag="VF517_02135"
     CDS             complement(43169..43399)
                     /locus_tag="VF517_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051760.1"
                     /translation="MGYAYGLAAIVFAAMAAIVAADGQPLGGLGLGAVAALVAYATFR
                     RDHEDDGVAPRYMAVFAFALVGLTVVLAVASA"
     gene            complement(43462..44400)
                     /locus_tag="VF517_02140"
     CDS             complement(43462..44400)
                     /locus_tag="VF517_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX8051761.1"
                     /translation="MLPDGFRVGHWSDSDGATGCTVVLAPEGTVAAGEVRGGGPGTRE
                     SELLSPAANAPGVQAVCFAGGSAFGLAAADGVVRWLEERGAGYSTPAGLVPLVAGAIV
                     YDLTLGDPKARPGPDAGRAACEAARAGEVERGSVGAGTGAAVGKVLGHEAWTKGGLGL
                     ASDSIGGARVTALAVVNAVGEVIAEDGAVLAGAWRDGGYVRTVDALKEGFGPPASARE
                     STTLVCLMTDAELDKRAAWLVARAASAGVGRAVQPSATAHDGDLVYCLASGDVEAAPF
                     AISALAADVTAAAIRDAVRSAAGTPVCPSAADRASS"
     gene            44436..44942
                     /locus_tag="VF517_02145"
     CDS             44436..44942
                     /locus_tag="VF517_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8051762.1"
                     /translation="MAAPRKPRPEPPKKRTQATREAIKRTTRVASARDAARTLEGPAA
                     ERQPRGGDRDPFTGELRKRGILPMLVLHFMSEGPSYGNQLIERISAVTVGVLHVNPNT
                     MYPLLRQLEAKGLIEGRWEHPERRSRRYYSLTVDGRREYKRLVKDVRPFLDSIARSIH
                     EIVREVYD"
     gene            44942..45394
                     /locus_tag="VF517_02150"
     CDS             44942..45394
                     /locus_tag="VF517_02150"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8051763.1"
                     /translation="MGTVREAISVRLDPQRAFRLWTDVRRWQTFVDGFARVKRLDDEW
                     PGAGAKLVWESTPEGRGTVTERVTEAEAGSRLVTHVFEERCAGTQTVTFEPDPEGAVA
                     QIEYEYELARGGPLRGITDVLFIRRAQSDALVRTLRRFATEAAEEAEL"
     gene            45430..46302
                     /locus_tag="VF517_02155"
     CDS             45430..46302
                     /locus_tag="VF517_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8051764.1"
                     /translation="MYVFKAAVVGAGTMGGEIAQVVASAGLQVVLKDVKQEFVDAGLE
                     KARGVTTGQIGKLVEKGKITQDQADQQIEEIVGRITGTLDYDGFGDVDFVIEAVPEKM
                     EIKQSVFAELDAVTPGHAILSSNTSSLSISEMAEATQRPDKVVGFHFFWPASMMRLIE
                     VIEGDETSEETMQATANFAQQIRKTAVRCAEVPGFVVNRILNSAASEIWRVTDEEGID
                     PQEVDKVIQESKTIPMGPYFLADMVGLDVTLHTAEHIAESYGDRFYVSERLKELVGAG
                     DLGQKTGKGFYEHG"
     gene            46289..47395
                     /locus_tag="VF517_02160"
     CDS             46289..47395
                     /locus_tag="VF517_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8051765.1"
                     /translation="MSTASTESQSLGTLVERFGLKTLVESCLVLEEGVASMKDIDIGM
                     MMGAGILPGPFQRADAQGLDEVLAALERAQAEWGEHFAPPLILRRLVSQGRLGQKTGQ
                     GFFPYPQPDADSSYETLAIETRGEVTIVWLNRPPANPLSPQVIRELADAWGKLDGKAR
                     AVVIASSNPFTFSAGADIKEFTKMNPDAEGRELIESGHGLMTSMEKSSTVTIAAINSL
                     AFGGGCELAMSCDFRIAAESASFAQPEINLGIIPGFGGTQRLPRLVGEGKALEMNLTG
                     DAIGAYEAFRYGLATQVVPDHELFDTALAWARKLSEKAPIAVEQIKSVSAMGELGEGL
                     EAEAHGFAKAFGSEDAKEGIGAFLGKRTPRFQGK"
     gene            47396..48157
                     /locus_tag="VF517_02165"
     CDS             47396..48157
                     /locus_tag="VF517_02165"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX8051766.1"
                     /translation="MAPAVGTSAAVRLAELLRGAERAVVLTGAGVSVPSGIPDFRTPG
                     KGIWEKVNPMEVAHIDAFRRSPDRFWQFYSDRFASLVDKRPNPAHEAIAELELRGIVR
                     GVITQNVDRLHRMAGSRKVIEMHGSIEHCVCLECGGRVALDDVIEILARHPGAPECTA
                     CIHPLKPDVVLFGEMLPAAAMAEAHALAAQADLMVCVGSSLEVFPVASLPGLTLESGG
                     RLALVTQGPTPYDGDAELKLTGDVADELQAVLSAL"
     gene            48217..49104
                     /locus_tag="VF517_02170"
     CDS             48217..49104
                     /locus_tag="VF517_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX8051767.1"
                     /translation="MDPKVRNVERKLARIASRSWGVVTVRELLSAGVSAQEIKRRVEK
                     GLLIREHRGVYRVGHRAPSVEATYMAAVKACGEGAVLSGRSAAYLQGLLRKCDPPAPE
                     VTAPTERNVKGVRTRRRHLDPRDTAKLHGIPITTVPCTLVDLAAELSLDDLARACHEA
                     GVRYRTTPRQVEAVLARRPNAPGGRNLRRILHGEARVTLSKLERRFLTLLRDAVLPLP
                     ETNRPAGGRRVDCRWPEHRLTVELDSYTYHGSRHAWEQDRRRERAAYARGDQFRRYTY
                     GDAFEGPAPMMRELRALLG"
     gene            complement(49101..49310)
                     /locus_tag="VF517_02175"
     CDS             complement(49101..49310)
                     /locus_tag="VF517_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051768.1"
                     /translation="MSRKAVTEIHDRRSRRVSRAIAARRRIDRAPLPARFGGEPARTG
                     GRVWVNGVEMGGTDPRHEALAASHD"
     gene            complement(49454..50326)
                     /locus_tag="VF517_02180"
     CDS             complement(49454..50326)
                     /locus_tag="VF517_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051769.1"
                     /translation="MEAPPPGARFRFVQFEFAFPLGPADGRYMTRADPEAEPERVLVL
                     RTSGAAPRPLVGRRRARRSGGDDGPLPVPVVRATVIDAEPLETDAGAAEWLDRLRRDR
                     DALEAEAGAAARDLNALLRAHRAAALDPSARDVAPSGANVVRVGYGSGEQVADGHFAE
                     AYELPLEDDTSKVRRRAAALAPDERLAAILGGRDELLACEELVLRARADLDAARPREA
                     ALQARIALEALLAELGGDAVGDLAADREPVGRAANEALAGSPGTELSAAVAAAVGRME
                     TALRRRRLGHVTNT"
     gene            complement(50331..52823)
                     /locus_tag="VF517_02185"
     CDS             complement(50331..52823)
                     /locus_tag="VF517_02185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX8051770.1"
                     /translation="MKRRLFFAILLAAVVASALAIFSMSASAQLRTFLVKLPTGNVIR
                     VTVDAPAGVPMDQVPGLPGTPIQELTPPGGGDEPAAPSGGGDGGGDQGGDKGTKPPAS
                     SGGGDQGSGSDKGSKPSSDGGSDQSRDRGDKKKDPSRADEPQSKAEEERAKERKKKAK
                     ERDRERTPLRNQDGSPTRTNPTFFDALPNPTPGTAVPNFVIRKFQVPIFLLPIYQAAG
                     IQYGVRWEILAAINEIETDYGRNLNVSSAGALGWMQFMPLTWKAYGVDANDDGKKDPY
                     NPVDAIFAAANYLKAAGAEKDIRRAIFAYNHADWYVDSVMLRAKLIAGVPADLVGSLT
                     GLTQGRFPVASRARYANDVLERELKKRVKRGENAANVLEDADRRSIDIFSDNGAPVVA
                     SNDGVIKKIGTDPKLGHYIVLQDVYGNQYTYSGLGSVSQLYPVPKVDLDIEDSSAKAV
                     AANMSDPRPGAPASAGRQASEKPDAASDSVTTEASDSTARVTYKPRIFAHPYRSKPRA
                     NGAIEQIFSRATSQKGFATYRGLFAHVYGLNAENAVLRPLKKGARVVAGTMLGRVGRP
                     DAQKAAHLTFSIRPAGRGAPTIDPKPILDGWKLLESTAIYRANGKNALYSSSDAFSIG
                     QILLLPKNLLQKRVLSDERVDIYPGGRNDIRSGQIDRRVLATLEFLAESGLRPTVTSL
                     RTGHGKMTSSGNVSHHWSGNAVDIAQLNGVPILGHQDKGGVTEQGVKRLMLLQGTMRP
                     AQIISLLDFGSNTLSMADHNDHIHVGFQPMFGENKKLGQQALAVLKPGQWSSLIDRLR
                     ELDNPVVPTKPSKYALPADKKNKRSSSAHRGE"
     gene            53298..53789
                     /locus_tag="VF517_02190"
     CDS             53298..53789
                     /locus_tag="VF517_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051771.1"
                     /translation="MDEEAQTDEGIRDRLTRQGEEALGKLAEELVGNPLLGGAVSRAF
                     EAREKAVQAQEAAMGALGIPSAADVERLTRRLRSVSQRLEGVEDGVDRLDARLERIEA
                     GVTALDGQLGGADADSGQATAAIELRLDEIARDLSALREAVAPGSEPPPRAQERLTVS
                     DAQ"
     gene            complement(53801..54493)
                     /locus_tag="VF517_02195"
     CDS             complement(53801..54493)
                     /locus_tag="VF517_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051772.1"
                     /translation="MPEPTPHLNPAAELAERVLLPGDPQRALTMAQALLESPRMFNTR
                     RGLWGYTGRASDGAALTIQSTGMGGPSAAIVVEELIRLGARRFVRTGTCGALDAGLRL
                     GDLVTVAEAIAADGTSRALGAQERVEADAELTAALAGGGGRAVTVVSSDLFYDPDPAR
                     ADGWRRAGAAAIEMEAAAVLTTAARRGARAGCVLLVTDLLAGGGRERMEHDEIDKRSP
                     RLGEVALRALAA"
     gene            54531..>54832
                     /locus_tag="VF517_02200"
     CDS             54531..>54832
                     /locus_tag="VF517_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051773.1"
                     /translation="MAERKSPAERLPDALREAVERTVQATRESVEATRESAKGTRGRA
                     QEAVDDLVKGAEAGAETLRDRVRGAVPATSEDLKDLRRELRAIGRRLDAIEERLPA"
BASE COUNT         8886 a        19791 c        18967 g         7188 t
ORIGIN      
        1 aggacggagt cgctgcgctc ccgagctccg gagtccggca gatcgagctc ttctccggca
       61 tcgacgtggg ctcgctcacc gagcgcctcg cgagcccggt gcaggactgc ccgtccggca
      121 actgcacgca gacgtgggac acgactttca gccccgccgc gggtgaccag agccgcacgc
      181 cgcggcgcat gcgcgtccag gccaccgacc aagccggcaa caccagctcg gatcgccagc
      241 gcgcgccggc gcccgcggtg cgctggcggc tggacgacac ggtcggcaac atcgccgccg
      301 actcggttcc gcgcgcggag caggtcacga acacgagctt cgagaccgac acgagcggct
      361 gggccagcgt gggcgccttc ttcctcggca actcgacgct cacgcgcatc gccgacagcg
      421 gcgcgccgca cggcggatac gtcgcgaagg tcgtggcccc cgagtggcag cgcgggatcg
      481 ggaccgacgt gaccgtcacg gccggggttc cctacagggt ccgcgtcaag gtcaagcgcg
      541 acccgggcgg tcaggagatc cgcatcgggc tcggcgagat cgccgccccg aacgaggagc
      601 tcgtcgtggc cagcaacgcc acgccgagca cgaccggcta ctcggactac acgggcgtgg
      661 tcgtgccgaa ccggtccggc acctaccgcc tgtacgtccg cgcggcgaac tacggcggac
      721 ctccgacgac cttctacgtc gactccgcgt cggtgaaggt ggactacggc gggacctacg
      781 cgggcggcta cacccaggac cggcccggcg cgacgaccga cggcgacaag gccgtttcct
      841 tcgacggcac gaacggccgg gtcggcaccc actacaagcc gttcgtcaac ggcaccgcga
      901 gcacattcga gggctgggcc aagcgcaccg acacgagcgg cacccacact ctcttcggcg
      961 gcaacgcgag tacgcgcgcg ccgatcctct acgtgccgga gggcagccgg gacatcctct
     1021 ggtactcaga cgtgggcacg gggcaaacgc gctggccgga tgccgcgccc acgggggcct
     1081 ggttccactg ggcgtacgtg ttcgacgagg tcgcggacac cgccgagctg ttcatcaacg
     1141 gggtgagccg gggcgtgagg acagtcaccg acccgtacaa cgccacgccg ggcaacctct
     1201 cgttcggggc atacgcgggc ggcagcaacg ggttcaaggg gtcgctcgac gacgtcgcgg
     1261 tctacccgcg cgcgctcagc gccaccgaga tcgcgcggct ctacagcccg gatccctccg
     1321 acgcgcgggt ggcgtactgg cggctcaacg agagcaccgg caccacggcc gccgactcgg
     1381 cgccgcatca ccagcacgtc tcgaacacga gcttcgaatc ggatacgagc ggctgggcca
     1441 gcaccggtgc gttcttcctc ggcagctcca ccctgacccg tgtggccgac agcgaggcgc
     1501 ccgacggcga ctacgtcgcg aaggtcgagg ccccggagtg gcagcgcggg atcgggaccg
     1561 acatgctcct caccgccggg cgccgctacc gggtgcgggt gaagctcaag cgcgagcccg
     1621 gcagccagca gatcagggtc gggctcgggg agatcgcgtc gcccaacgag gagctcgtga
     1681 tcgcgagcaa cgcggtgccg ggctcggcgg gctacacgga gtacacgggc gtcgccaccc
     1741 cgagccggac cggcacctac cgcctctacg tccgcgccgc gaacgcgggt ggcccgccgt
     1801 ccaccttcta cgtggacgcg gccaccgtca ccgaggacaa cgccggcacc tacacgggcg
     1861 gctacaccct caacgagccg ggcgcgacgg ccgacgccga cagggccgtc aggctcaacg
     1921 ggtcgtcggg ttacgtcgcg gtgcccgacg cgcagactct cgacctcggc gacaggctgt
     1981 cgatcgaggc ctggttcaag cgcagctcgc tgaccgggga ccagatcctc gtcgggaagg
     2041 gacaggacgc ctacgtcctg atgatcgccg gcggcaagct gacgctgcgc aagggaggcg
     2101 ccggcaacat cgtgcagtcg accgccacgc cgggggtcga cggccagtgg caccacgtcg
     2161 tggcgacgaa ggacgggccc gccgcgaaga tctacctcga cggcgtcgac gtgtccggcg
     2221 cggtctcgac cctgaccctc gccaacaact ccgcgccgct caacatcggg accagcgcgg
     2281 ccgccgatcc ggagaagtgg aacgggtggg tggacgatgt cgcgctctac tccgccgccc
     2341 tcagcggcgc cgacgtcgcg cgccttcaaa agcagcacgt ctacgactgg cgggtcgtct
     2401 tcgacaacgg tgcgccggtg ctggacgcac ccgtgcgcac tccgtcgggg accgcctggg
     2461 tcaacgggac gacccagatg caggcgacgc tcgccgcgag cgatcccgtc agcggcatca
     2521 agcgcttcaa gctgttcacc gccaagcagg gtgggggcga ggacctccag accaagtcgt
     2581 acgacggttg ctccgagaac gtggacaacc tctgcgacac ctcggagcgg cacacgttca
     2641 cctacaccgc cgggaacatg cccgagggca gcgcgcagcg ggttcgcgcg gtcgccgagg
     2701 acacggtcgc caacgtgtcg gccgagcgca gctgggacgt caaggtcgac cgcaccgggg
     2761 ccaacgtggg ccccgtaacc ggcccgctgt cgacgaccgg tcgctgggtc aggcccggcg
     2821 tgaactacag cttcgagtcg ggggcctccg actcgctctc cggggtcgag tcgatccagt
     2881 tcttcgtgaa cgagcagctc caggccaccc agcccggcga caactgcacc ccccagggct
     2941 gcgcgtccaa cccctcgccg aagacgttca gctggaccgt gcccgatccg tacacggggc
     3001 agggcgaccc gtcgctgagg gtgaaggcca acgacctcgc cgggaacccg ggctcgtccc
     3061 gtacctggaa cgtccgggtc gacgacgaca agcccgccgt gccgaccgtg accggcacgc
     3121 tgaaggacgc cgagggcaaa ttcgtgaacg gcgcgagctt caccgtccac gtggacgcga
     3181 gcgacggcac gtcgcagagc ccgagctcgg gcgtcaagca gatccgcctg ctcgtgggcg
     3241 cggacccgag ctcgctcacg ctgcgcaagg agagcccggt ccagacctgc gcgacgcaga
     3301 actgcacgca gccctgggac acgaccttca gcccgctcag caccgacgag acgcgcgcgg
     3361 gccggctgat ccgcgtcgag gtcgaggacc agatgggtca cgtcgagaag cgagactggt
     3421 acgccggctt cgacaggacg gcgccgtcgg cggcctcgcc gacccacgcg gaccccgagg
     3481 actggtggta ctcggagacc gcgcagcgga cggcgaccgt caacgcgacc gattcgacca
     3541 gcggagtggc cagcgtccgc ctgtcggtgc cgcagctggg gggcggaccc tcggtccagg
     3601 accatccgtt cggttgctcc aacgtggtcc cggccacgtg cgccacctcg ggctcgcacc
     3661 cgttcggcta cggcctggcg aacgtgcccg agggacaggt gtcggtgacc ggcagggcga
     3721 ccgacgtggc cggaaacaac agctgggacg tgacgcgcac cgtgaaggtg gacaagtcgg
     3781 cgccgcagtt cgtctcgaag tccggcaccg cttccgtgga cgggctctgg ctcgacgacg
     3841 gcgagcacga cgtgacggtc gactggaacg acgcgttctc gggcgtctcc tcgagccgcg
     3901 tcgccttcca ggcgccctcg ctcgccttgg acggcttcga gcgcaccgcg ctcgcgggct
     3961 ggggcgaggc cgacgcgggc ggcccgtgga gcgcgatcga gggcccggcg agcgacttca
     4021 gcgtcgccgc cggccgcgcg agcgtgtcct cgccgcaggg gagcccgcgg acggccgtgc
     4081 tcggctccac cccggcgcgc gcgttcatgg cgcgggtcaa ggtccggctg cccgacgtgc
     4141 cgccggcctc ggggtggtcg gaggccgcca tcgtgaacag ccattcgtcc ggcagcttcc
     4201 acgtccgcgc agccctcgag ctctaccccg acgggcatct cgagctcctg ttccgggaca
     4261 aggccaacaa cgagctcgcc cgcacggacc tcggcatgag ccacgtggcg ggcgacgact
     4321 actacctcac cttccaaaag ccggggggga tgagcatccg ggccaaggct tggaaggtcg
     4381 gcacgccgga gcccgcgttc ccgcaggtgt tgatctccga cccctacgtc tactccgggg
     4441 gctcgcggct gggcctgcgc gccatcaacg gcgccgctgt gacgcagtcc accgcgttcg
     4501 acgacctcga cgtccagccg ctgcgcagcg gggactacga ggagccgttc aagtcgggcg
     4561 gctgcgaccg gaccggctgt gccggcccca tgagccacag cttcacctgg agcacggccg
     4621 gcgagcccca ggggctccac cacgtctcgc ccaccgcggc cgatccggtc ggccaccaga
     4681 cgcaggggac gcagtgggcg gtcggcctcg accgcagcgg cccggcgctg gcgctctcgg
     4741 gcgcgctgaa ggacgccgac ggccagacgg tgtaccgcga ggacatggcg ctgcacatgg
     4801 acgcgcgcga cggccgcgcc ggcaacgacg ccacccgccg ctcgggcacc gagcggctcc
     4861 aggtgtgggt ggaccgcgcc ggcccgcagg acccggctga ccacgaccag agccaggcgt
     4921 gcacgccgga cagctgcgcg atggagggcg actacacgtt ctcgccgacc gagcacggcc
     4981 ccggggcgca cacgatcacg gtgcgcgcga ccgacaaggt cggcaaccag accgagcaga
     5041 ccgtgacggt cacggtcgcg gcggacgagg ccggccccga gctcgagctc tcgggcgacc
     5101 tgcggccgcc gctggctacc ggacgctccg tccacatcaa ggcgctcgag gaacggcccg
     5161 ccgacaccgg cgtcaggaag atcgagctct ggctcgaccc cgacctgacg cctacctccg
     5221 acgagcgcga gcacggccac gagccggact gctctgcggg ctgcccctcc gtggcggaga
     5281 ccgactgggc gctcgaccag gcgacgccgc ccggcaagca cgagatcctc atcgtcgcca
     5341 ccgacgacgc cggccacagg accgaggagc gctacgagat acacgtgctc gacctgctcc
     5401 cgtcgagccg gtcgaagctc ggcctcgagc actggttcga ctacgacgac accgccgcgg
     5461 gcggcgactc gtccgtctac gtgaacggcg agaccggcaa cacggtctgg cacagcgtgc
     5521 cggtcgtcaa ccccggtcgc ggcctctcga cggtcgtcaa cctgacctac aactcgcacg
     5581 accgcggcgg cgtgctcggc tcgacgctcg gccgcgtccc gctggtcgac ctgagcggcc
     5641 aggacctcaa caacgacctg cccgggctct cctactcgga ggcgggggtc gggttctcgc
     5701 tcgggatctc ggggcccacg cgcctgaacg agccacttgg cggcgtgctg atcgcgcagg
     5761 ccgtcgagga gggcgaggcg gcgatccccg gcttcaccgg cacgctgccc ggcgagagca
     5821 acctcgtcgt gacgatgacg gactccgacg ggacggtgca cacgttcacc aagggcgccg
     5881 acggcgagtg gatcgcaccg cccggggtca gcatgcgcct gcgccggttc cagaacggcg
     5941 gcacgctggt gaacccgatc gacgagaagt gggcgctcac ccggcccgac ggcatcacgc
     6001 acttcttcga caacctcggc tacctcacca agaccaagga ccgcaacgac aacgtcctcg
     6061 agtacatcta cgagccctac gacgcgctga ccggcaacgt caccaagagc gtcgtcacct
     6121 cgtccggcca gaacctcgac tacgcgctct gcgacggcgt gcagctcgga aagcagaccg
     6181 aggtgactct gcccgacggg accgaggtgc cggtgttctg cgcgaagcgc gtcagccggg
     6241 tcaaggatcc cgccgggcgc gagctgaaga tcgattacaa cgactacgac tcgagctacc
     6301 tcggcctcgc cgacggcaag ctccccatcg agttccgcgc cgacttcccg ggcctgatcg
     6361 gcgggcgcgc gggccggatc aacaagatca ccgaccacgc gaaccgcgag tacacgttcg
     6421 agtacgacga ccacggctac ctgaccaagt tcgtcgaggc ggcgaacagg cccgagaagc
     6481 ggacgaccga gctcgcctac gaggactgga agccgggcct cgaggcgatc ggccaggacc
     6541 gccagctcat cgaggtcaag gagggcgaga ccggccgcaa gactctgatc cggcacgtcc
     6601 acccggacga ccggaccacg ctgcccacgc cgggcgtgct gctgctcccg cgccaggcgt
     6661 gcggcgtcac gaagcgcaac gacggcgtcg gcgacctcga gtcggtgccg gtggcgaacc
     6721 aggagtgccg gacggccgac aacgacctcg agaagaccta cgactacacg gccccggagg
     6781 gctcgacccc acgcaagttc gaggtggacg agatcctcat gcgggcgcag gcggtggggg
     6841 agagcgcaac ggtcggcacc acggtccacg agatcgacga cgagggccgc cccgaggccg
     6901 tgaccgaccc gaacggggtc aagacggcgc tcacctggaa cgaggacgtc aacgccgtca
     6961 gctcgatcga ggaggcgagc gggacggacg acgcgtcgcg gaccgagctc gactacgacc
     7021 aggcaaaccg cacagcggtg atcgcccagc agcgcaagta cccgaacttc ccgagcacgg
     7081 acggcgcgcg cgtgacgaac ttctcctacc agttctcgaa cggccgccac cacagcaccg
     7141 ccagcggcgt cagcgacgcc gacggccagt tcgtcgcgga catgaccgag gtacgcaacc
     7201 cgaagaccgg caccggccag tcgttcgaga tcgagcaggt cgccggcgcc tacaccggca
     7261 acgtcaccaa gcgctgggac aagccgggta agagcggcaa cgtcgcgacg acgctctacg
     7321 acggccacgg ccagatcatc tccgaggacg acgaggtggc cgagaacggg ccgacgatct
     7381 acgagcagca cgacccgacc ggccagccgc agaaggtgct cgatccgagc ggcaagcggt
     7441 gggactacct ctacgacgcg gtgggcaacc tgacgaccgt cgtcgacccg cgcgcgaccg
     7501 accgcagcgg cgcggcgggc ctgccctaca ccaccacgct cggctacgac gcgctggacc
     7561 ggctcgtgaa cgagaggatc ccgcgcctgt cggccgattc ggatctgccc gactccaagc
     7621 ggttcaccct gcgcagccgc gagttcaacc gcaacgggac gctgaagaca gcgaccgacg
     7681 gcgagggcac gaccacgtcg atgacctaca cgccgatgga ccagccgctg cgcgtcaccg
     7741 cgccgggcaa ggccgggcgg cccgagacca ccgaccacgt ctacgacgac gcggaccggc
     7801 tgatcgcccg ggtcgagccg aaggggacgg gcgcgacggt ggcgacggcc ggcgagcacc
     7861 agaaggcgtg caccgacgca agcgtcgacc cccccgtcgt ggaccacatg actcggtact
     7921 gcctcgatcg cggcgggcag atgatcgcgg aggtgagggc ggcgacggtc ctcccgccga
     7981 acggcggccc gagcgccctc atcaagtcgt tcgcctatga ccgccgcggc aacgtgaccg
     8041 gcatcgtcga ccccctgcgg aactcggggc gcagcatccg cgaggcgacc gacgccgctc
     8101 aggtctccga tccgctgacc acctcgcgca ggctcacgta cgagtacgac cgcgtggacg
     8161 agcgcaccgc gcagattgag cacgtcaccg agaccgggcc cgacggccgt ctccccgcga
     8221 agcgcaccgc gtacgagtac gacgcgaacg gcaacaagct caccgtcctc ccgccgaagg
     8281 cgttcgtcgg ccgctccgcg agcgatcccg accgcgacta ccagacgaag acctcgtacg
     8341 accatcgcga tctcgaggtc gcggtgcgga cgcccgccgg ctgctcggcc taccagcgtc
     8401 gtgaggacgg ccgtatcact gccatcaccg cgccgcgcgg cacgaccgcc acggacgccg
     8461 acgactgctc ggcggccggc ccgttcaagt accacacgac gaagctcacc tacgacgcgc
     8521 agggcaacct cgcgtcgcgg accatcccgt tcgcacaggg gcagtacggc cgcgccgatt
     8581 ccgagttcga gaactggaag gtcacctacg agcgcgacga cgtcggcaac gcgacgtcga
     8641 tcgaggaccc ccgcgggaac gagttcgaca acacgttctt cgacggcggt cagctccgct
     8701 ccacgacgcg gccgtcctgg tacgagctcg agtggagtga cgaccacgcc ccccgcgacc
     8761 cggcgcagcg ctacaagcag gagaaggatg ccggcggcga cttcgagctg gccgacggcg
     8821 gaccggagat ccgcgagagc gagatcatgt cccgcaacgc acagcccgag ggcgagcgct
     8881 ccgacctgcc cgagacgcac ggacagggcg acttcggcaa ggtcgacccg caggatcccg
     8941 gcgacctcct cccggaggcg ggcgccacca acctcgacta cgaccgcgag atgcggctca
     9001 cgacgatcag cgacgcggcg ggcggcgatc ggacgatctc ctacgacgag gcgggccggg
     9061 tcatcggcaa gagctggccg ttcaagcccg acgacccgat cacgcacgac ttcgagtacg
     9121 acctgaacgg caacctcgag cggtacaccg acggtcgcgg caagccgacc acctacggct
     9181 acgacgggtt cgaccggcgg atcagcgaga ccacgcgcgg cgccgggcgg gcgccgggcg
     9241 acgcgttcac cgaggaggtc acgcgattcg cctacgatgc gaacgacaat ctcgagtggc
     9301 gtcagacgcc gcgcggcacg gcgctgcgat tcgacttcgc gtacgactcg accgaccacc
     9361 tgatcagcga ggccaacccc gccaacgaca cgtgggagta cgactacgac gtcaacggca
     9421 atcgggtcaa ggagcgctcg ccgcgctacc agagcgccac ggcgggcgac aagccggact
     9481 tcgagcggac gttcacctac gaccccgcga accgcatgac cgaggacgac ccgggcctcg
     9541 gtgacgggtt cacgacgtac gagtacgacg cggacggcaa cgtgaccgag atgaccagcg
     9601 gcggtgccgc ggatgccaac ggcgcggcct ccgcccggcg caagcagacg agctcgtacg
     9661 acggccgccg tctcgcctgg cgccgcacca cgtccacggc cgggggcgac gacaagcgga
     9721 cccagatcca ggagtacgac gccaacggaa acctgcgccg catgatccag gggaagggcg
     9781 tcgaggacca cagccgggcg aaggtgctcg acgcaccggg cacgtcgacg gccgacgtga
     9841 cctggcacgc gaccctgcgg gactacgacg aggacgacca gctcgtggcg gtccactcgc
     9901 cgtggagcag gccaccgagc gagggctcac ccgccgtcga cgacccggac gggccctccg
     9961 acgccgagac gggcggggac gacaggaaga tcgtccagga cttcctgcgc cccaccgcgg
    10021 gcaacccgct gaggcgcgtc acgtcgatag tcgcgccgca cgagtcgagc gacacggtgg
    10081 ccgcgcgcac ctcctacacc tacttcgcga acgggtgggt caagacgcag agcgacgaga
    10141 agctcgtccg gcccgggagc accgcgcccg tgcgcaccgc gaacgtgacc tacgactacg
    10201 acaaggccgg gaaccagacg gtctggaaga cggacgaggc gggcgacagc ccggacggcc
    10261 ggctcgtcaa gcgcttcttc tacgacgacg ggactctcaa gcgccgcgag gcctacaaga
    10321 ccaaggccgg cacggacgac gagacgaggc ggacctacga ctacttctac aacgagaacc
    10381 gctcgctggt gcggatcgac gacttcgacg caaccgccgg cggaacgggg cgtgctcgct
    10441 ccacgaagat caagcgcgac gaggccgagc gcgagagcgt cgtgaacgag gactgggacg
    10501 gcggccggga caccgtcctc gagtacgacc gagcgggcaa cgtgacgacc cgcaagaccg
    10561 acgggatcta cgtgaacccg acgacctaca acggcgacga gcgcaaggtc acgacgttcg
    10621 actacgacgc gctcgaccgc gagacctccg cggtcgtcga tccggccgag ggcgacacgc
    10681 ggacgacgac caccacgtgg tgggactcga acgacatgaa gacccgcacg aagtcgaacg
    10741 gcaccgtcga gtcctacttc tacaacgcgc gcggggagat ggcgcagaag cggcgcaacc
    10801 cggacgaggg cgacaccgag acgcaggact acgactacga ccgcgacggc aaccgcacgc
    10861 aggacgagcg cggcacccac gtcttcaacc cgcggggcca gctcgtcaag tggacgcgcg
    10921 ggccgaagta cagccactcc gagcaggcga aggacaagga cgggacggtc gtgacctacg
    10981 agcgcaacgc cggcggagac gtgacgaagg aggtcgacaa ctggaagccc accggcatca
    11041 cgggcgacac gaccaccacc taccgctacc acggcgagcg cctgatctgg accgagacct
    11101 cgcggcccac ggtctctggc acccagacga ctcactccga ctactcgtac gacgacttcg
    11161 ggtccgtcac gaaggtcgtg cacgagcaga cgggccccgg cagcgacccg aacgagcctc
    11221 cggacggtcc gccgtcgtcg gtcagctgct ccgagatgcc gaacgaggcc gaggcgaagg
    11281 tgacccgcta ctgctacgac gagttcgagc gacgcgtcca gtcgcgcggg tcggggatcg
    11341 agaagccgac gatctacgtc tacgacggcc tcgaccgccg cgaccgcaag acggtggagg
    11401 agaacggcga ccccaaggtc cacgagtacg cctacgtggg cctgaacgac ctcctctcgc
    11461 gggagaccga caaggacggc aagaagaaga cctacgacta cgactcggac ggccgccggc
    11521 tagggcagtc ggtcaaggca tcgggggact cggcgccgac gcaccggccg tacgcgaccg
    11581 acgccagcgg ctcggtggag ggtctcgagg acacggaggg gcgcttcggc gagctgaacc
    11641 gggacaccta cctgtacgac ccctatggcg agtccgagaa cgtcggcgga ctcaacgaga
    11701 cggaggaccc cggtctctcc gacgccgcga aggagaaccc gttccgcttc caggggttct
    11761 acttcgactc cggcgtcaag acctatgaca tgcaggcccg cgactaccgg cccgacatcg
    11821 gccgcttcct ctccgaggat cgctacgagg ccgcggtcgg cgacaagttg ctccaagcgg
    11881 atcccctcac gcagaaccgc tacgcgttcg ccggcggcaa tccagtcaac aacgtcgagt
    11941 tcgacggcca ccacatcatg cgctgctaca gcgccgacca ctgcagcggc gcgtacgagc
    12001 actggcgctg gcaccgccgc caggcgcaga tccagaagca gcgccggaag gacacgatcg
    12061 tcaagaacgc ccagaacaac gcgcggcagg cgctggccca gggcgacgta aagcgctact
    12121 accggaacct cgcggtgatc atcgagcagg accaggccaa gctcaaccgc cgcgtccagg
    12181 cgcttcaggc ggccgtcggc ccgctcgagg ccgcccagcc gaagaagccc gggttctggg
    12241 actacgcgaa ggtcgtgttc gacccgacgg acccgctgga catcctgctg acggccgcgt
    12301 ccgccattcc cacgggcgcc acgcagggcg cgaagggagc caagatcgcg ctcaagggcg
    12361 cgaagctcgc cgcccgggtc gccaaggccg ggaaggtcgg caaggcgctt tcgaaggtcc
    12421 gcaccctgct ggcgggcgcg gtgtcgcgcg cgggtgacaa cgcgagcctc gtgaatcgcc
    12481 tgcggcagtg cggcaacagc ttcacggcga ggacgcccgt ggtgatggcc gacggctccc
    12541 gcaagccgat cgcccgcgtg cgtctcggcg actacgtcgt cgcgacggac tcggccaccg
    12601 gtcgcacggt cgccgctcgg gtcaacggac tgctgcgctc ctcggcgggc aaggacctca
    12661 cggtgatcac cgtcggcggg cgccggatca ccgcgaccga tcaccatccg gtgttcgtcc
    12721 tgcagaaggg ccgctgggtg gacgcggcgg acctcgaggt cggcgatctc ctgctcggcg
    12781 ccgacggcga gcccgttccg gtgagcgcgg tgcgcgacgt ggatgccgac gtccccgtct
    12841 acaacctgac cgtcaacggg atccacacct actacgccgg ggaccggccg gtgctcgtcc
    12901 acaactccgg ctcgtgcgac gtggacgccg cgctcgaggc cgccgccgcc gagctcaagc
    12961 agctgaaccg cggcaagaat cgagttacca tcaacacccc gggcggtcgg atggtggtcg
    13021 acctccaggg caaggcccac tactccaagg cacagggccg gaacgtgccg actccccacg
    13081 tcaagatgca gacgcggaac cagaacccgc tcgacccgaa ccagttcacc ttctccgact
    13141 cggacgtctt ctccgcgggc tggcaggaga tccggatcgt gcggcaggcg ctcagccgtc
    13201 gcgccggcaa ttgaggttcg tggggctgcg cgagtggccc atgaacgagc cggtgatcga
    13261 gtgcgagacg gagtcgcact gctacgacct ccacaacgcg ggcgactttc gcggggtcga
    13321 gtggacgccg ccgggcgccc tggcgttccg cttcgtgtac gaccccgacc acctgcacac
    13381 gcccggggag ggcgacctac gcacggcgct cgagctcagg ttcaccggcg tacgtagcct
    13441 ccacaccgtg ctcgcgccgg acttcgcggt cgagagctcc gccctggtag agcactgggt
    13501 ctggaaccgg gtctccgacg agctcggcgg cttcgagttc aaggtcggcg agctgacgct
    13561 gaacttccgc gcgaccgcgg tcgagctgat cacggcaccc gagccgctct agccccactc
    13621 cgggtacacg gcgtccccct caagccgccg ctcccagcgc cgatcacaac ggtgcatgga
    13681 ttggaccgag gctcacgtcc ggcggctgtt ctggcgcgcc gggttcgggg cgacgccgca
    13741 ggaggcggcg cgcttcgcgc aagctggcat ggacgccacg cttcgcttcg tgctgcgcgg
    13801 tgacggtggc gccgagctcg agggcccgga gcccagcgcc gacggccagc cgctcgaccc
    13861 ggtgaacgag tggggccacg acgtcctctg gtggctcgac cggatggtcc gcagccgccg
    13921 cccgctcgtc gagaagatga cgctgttctg gcacgaccac ttcgccacga gcggccagga
    13981 caccccgctg atgctgcgcc agaacaagat gctgcgcaag cacgcgctgg gggacttccg
    14041 cgcgctgctg aaggacgtga cccgcgacca cgcgatgcag ctcttcctct cgctggccgg
    14101 ctcgaccaag tgggccccga acgagaacta cgcgcgcgag ctgatggagc tgttcacgct
    14161 cggctcgggc tacaccgagc gcgacatccg cgaggcagcg cgcgccctca cgggcttcga
    14221 ggcgcgctgg accgacgacg gcttcaaggg catccgctac cgcaaggact ggcacgacac
    14281 gggccagaag cggatcctcg gcaggcgcgg gcgcttcgac tggcgcgacg tctgcgacat
    14341 cgtctgcgag cacaggcgcc acgcgccgtt cctggtcgag aagctctggg cgttcttcgt
    14401 caccgagccg ctcgacccgg gcaccaggag gtcgctcgtc aagacctata cccgcgggaa
    14461 gctgcggatc gagcccgtgg tggcgcagat tctcgcccac ccggcgctct acgcgcggct
    14521 cgaccggccc gacatggtca aggcgccgat cgtctacctg gcgggagctc tgcgcgccac
    14581 cggcgccggg gtgcagcgcg agtcgtgggt ctggctgtcg gagcagatgg gccagcagct
    14641 cttccacccg ccgtcggtcg cgggctggga ctggggcccg gcctggctct cgacgaacac
    14701 gatgaaggcc cgcttccaga tcgtcaccta cctgatgacc gacgacggcc cgctcgaggt
    14761 gcccgacaag tccgtggcga tcggcgtgcc cgtgggcgac gcgctcgaga gcgcccgcgc
    14821 ggccaccggc cggccctgga cctcgcccga gaccgacgcg gcgctcgatc gcctcgccac
    14881 gacgatggtc gccggcctca agtcctggga gcgccagcag cgcgccgatc agcgcgagcg
    14941 cgccctgcgc cacctcctcc tctccggccc cgacgcccaa ctgcactgat gcccaagagc
    15001 acccaccgcg cctgcgacga tttccacgcc accagcgagg ccacccgccg ctcgttcctc
    15061 gactcgggga ccacccgccg tcagatactc gcgcgcggga taggcgccgg gctcgccgtc
    15121 tacgcctcgc gcgccatgtc gatcgccgac gtgttcggtg ccgcagaggc ttccgcagca
    15181 tccggacccg aggccccgat cctcgtgtcg gtcttcctgc ccggcggctg cgacctgctc
    15241 gacagcttcg tcccgctcga gcagtatggc cgctacgccg acctgcgccc gaagctcaag
    15301 cagaaggacc ccgcggcgct cggctccacc ggcctcggcg tgcatccctc gctcgcgtcc
    15361 ggcgccggcg gcggcatcaa ggggctcttc gagcagggca agatcggcct gctcccgggc
    15421 atcgactacg ccaacccgga cctctcgcac ttccactccc gccacttctg ggagacgggc
    15481 ctgatcacgc agaccgccgg gactggctgg ctcggccgga tgctcgacag gagcggttcg
    15541 gccgacaacc cgctccaggg catctcgctg tccggcacgc tctcgccgct cctgcgcacc
    15601 gccggtgccc ccgtcgcggc cgtccactcc cccaacgccg ctcagttctg gatcccgggc
    15661 atgtggggga gctcgcagga gatcgcgatg caggcctggt cggagatggc cgagcgtcag
    15721 ggcggcggcc cgggcgagcg cgccgccacc acctcggccc ggctcgcgaa gcaggtcgcg
    15781 gaccggctcg cgccctaccg ccacgacgac gacgagccga acccgctggc ctcgcccgtc
    15841 gagtacccga aggacaacga cctggccgag cgcctgtccc agctcggcgg gctcctctcg
    15901 cagccgctcg gcatccgcgt cgccgccgtc gaggccgagg gcgagttcga cacccacgac
    15961 ggccagccgg ccgacctcgc ccgcgggctg aaggacgtca gcgaggcgct ggccgcattc
    16021 caggccgacc tcgaggcgcg cgggctcgcc gatcgcgtcc tgacgttcgt gtggtccgag
    16081 ttcggacgcc gcccgcagga gaacgagtcg tccggcaccg accacggcgc cggcggcctc
    16141 gcctgggtcc agggaacccg cgcgcgctcc ggcgtgctga gcgactaccc ggacctcggg
    16201 cggctcgacc gccaggacaa cctcgccgtg accgtcgact tccgccgcgt ctactgctcg
    16261 ctgctcgagc agtggatggg ggcggacgcc ggagccgtga tcccgaacgc ggccggcttc
    16321 ggccgcatgg agctcgtctc gtgacgcgcc ggcgcgccat tgcgggcgcc ctggccgcgg
    16381 cggcggccct ggcggccccg gccgcatcgg gcgccgcggg ctccggcgac gggccgctcg
    16441 acgtgcgcgg caccgccgtc ggggtctccg agcgcgagtt ccggatctcg ccgtaccgcc
    16501 ggacggtccc aaccggaacc ctcaagttca acgtgaggaa cttcggcgag gacgtgcacg
    16561 acctggtcct cgtcagcccc cggggccgga agctcggctc cacgggcgcg attgcggcgg
    16621 gggagcaggg cgtcctgcgg atcaagatca agacgcccgg ggtctaccgg ctcgtgtgca
    16681 cgcaggcgga tcacgccgcg cgtggaatga agagccggat cgccgtccgg gcgactcgca
    16741 gatagggcat cccccgattc ggggggcctc acatcccttt aacaaattgc ctctagcgaa
    16801 ccgccgcgac cgatgttacg gtcaccccaa tggggggctt gtggcccagg cccaccatgc
    16861 cgatgggact cgtgacttga gtctgagact ggctactccc tcacccctcc gccggacccc
    16921 cgccctcacg gcggccctgc ttgcatgcct gatgctgctg gcgctggcgt cgagcgcctc
    16981 cgccgcagtc acgcagaagc aggcggccaa gaaggccctc gcggcgctcg ggtccgagaa
    17041 gcggagcgac gcggtcgtcg tcttcgggtt gccgaccccg ctcaaggccg gcaccaaggt
    17101 cacccaggcc ggcaccgcca agtccacgaa gggctctacg aaggggatca gcagccgcct
    17161 gcgctccgct ggcgtgcgca tggtcaaggc cccggcggtc ctcaccgtca ccaagcgctc
    17221 gtacttcttc tatgaggacc gcggcccgtt cgagctctac cagcaccccg gccgcgtcgc
    17281 gctcgtcgac gcccagaccg gggcggtccg gctgtcccgg acgatcacct ggccgccgct
    17341 cctgaacggg cggcttcccg cgttcctgaa gagcgccggc ggctaccgct cggccaagta
    17401 ccaggtcctc taccgcccgt acgcgatcga cggctccgcc cagcagccgc gcaccgcgaa
    17461 ccgcaacctc ttcgaccagg acccgtttgg ccccggacgt ccgcttcctg acatggcgaa
    17521 ctccaaggcg atcgccgacc ggctcgccgc ggagcactcc tgcatcctgc gcgtgaccga
    17581 cacgctgccg tccttctgga acgtcaacca gctcaacctc acccgcacgt acgtggggac
    17641 gctgttcgag aagctcgagc gcgacaacgg cggcttcatc gatgatcgct acggcgcccg
    17701 ctccggcgag accctcgccc agagcgtcga ccgcctcgtg gcggccgggt gcaaggacat
    17761 gctcctctac gtcgccggcc agggctacga ccagggcggc gacccggtga tccagctcgg
    17821 cacctcggtg atgaagggcg gcctgctcgc gcagcagaac ctgtccgcga gcgagctgcg
    17881 caagctcgtg tccggccgcc ccggcgtgac cttcaagttc aagttcgacg cgccgtactc
    17941 gggcgggttc atgagccgcc tgcgcgacct gtcgaacgtg ctcctgatcg agacgcccgc
    18001 cgccgagggc gacaccgcgt tctcgttcat ccccgaggtc gcgcagaacg gccagatggt
    18061 ctcggcgccc tcgaaccccg ccggcctgct cgagttcacg aactgcgagc tcaacggctt
    18121 caaggccttc ttctcctcga aggaggagat cgacagggcg acgcaggcgc agaacgaggg
    18181 cgtctcgttt ctcgccgaga tgctcgcccg ctcgcaggag ctgtgcaagg gcgacgggtt
    18241 cgccgacgac ctcggctcga acccgcagct ctacgccgcg aagcgcggct ccggcaaggg
    18301 cacgaacctg ccgccgaagg ccgacccgca gagcgtcgcg gccaccgagg acacgccgaa
    18361 ggccatcacg ctcaccggct cggaccccga cggcgatccg ctcaccttca cgatcaccaa
    18421 gcagcccgac cacggcacgc tctcgggcag cggcgcgaac gtcacgtaca cgccggccgc
    18481 gaactacaac ggcccggacg acttcagctt caaggtcacc gactcgaagg gcgcggagag
    18541 caacaccgcg aaggtctcga tcgacgtcgg cgccggcaac gacgccccgg tcgtgaccaa
    18601 ctcgcagaac gccacgccct acaccgagca ggccgccgcg gtggtcgtgg acggcaacct
    18661 cacggtctcg gacgtcgacg acacccagct cgagggcgcc acggtccgcg tcagctccgg
    18721 cttccaggcg ggcgacaagc tcaacttcac caacacctcg gcgatcaccg gcagctacaa
    18781 cgccggcacc ggcgtcctca cgctcaccgg ctccgacacg gtcgcgaact accaggccgc
    18841 gctccgctcg atcaagtacg agaaccagac cgagaacaac ccctcgagcc ccaagacgat
    18901 cgagttcaag gtcaacgacg gcacggccac ctccgcggcg tccaccaaga acatcgcggt
    18961 caccggcgtc aacgacaacc cggtgctcga cacgaccgac gcggcgctcg cgtacagcga
    19021 gaactccggc gcgcaggccg tggacaacgg gatcacggtc gcggattcgg actcggcgac
    19081 cctggcgggc gccaccgtcc agatcaccgg caacttcgcg cagcccgagg acgacctcgt
    19141 cttcaccaac cagaacggga tcagcggctc ctacaacgac gcgaccggca ccctgacgct
    19201 gagcggcacg gcctcggtgg cgaactacca ggcggcactc cgctcggtca agtaccagaa
    19261 cacgtccgac aacccgtcga ccgcgacccg cacggtcagc ttccaggtca acgacggcgc
    19321 cggcgcgaac aacctctcga acgtcgcgac gcgcgacatc agcgtctcca cggccaacga
    19381 cgctccggtc gtcacgacct ccgcgggcaa cacctcctac accgagggct ccccggccac
    19441 gacggtcgac ggctccctga cgatcaccga cgccgacgac accaacatcg agggcggcca
    19501 ggtccggatc tcgtccggct tccagtcggg ggacgacctg gtcttcgtca acacggcgaa
    19561 catctcgggc gtctacaaca ccggcacggg tgttctgacg ctgtcgggca cggacacggt
    19621 cgccaactac caggccgcgc tgcgctcggt caagtaccgc cacacgggcg acaacccggc
    19681 gacctcgaag acggtcgagt tcaaggtcaa cgacggcgac gcggactcga acctggccac
    19741 caagggcctc gcgatcacgc ccgtgaacga cgcgccgacg gtcaacacga ccgcgacggc
    19801 gctcacctac accgagggct ccggcccgca gcccgccgac tcgggtctcg gtctgagcga
    19861 tccggactcg acgcagatcc agggcgcgac ggtccagatc accggcaact tcgtctccgc
    19921 cgacgaccag ctctcgttca cgacccagaa cgggatcacc ggctcctaca acgacacgaa
    19981 cggcacgctg acgctgagcg gcacgagcac ggtcgcgaac taccaggccg cactccgctc
    20041 ggtcgcctac gagaacgtga acgacaaccc gagcgggacg aagacggtca gcttccaggc
    20101 gaccgacgcc gagggcgcgg cgtcgaacgt cggcaccagg gacatcaact tcaccgcggc
    20161 caacgacgcc ccggtcgtgg acaactcggc cggcgcgacg tcctacaccg agggcgatcc
    20221 cggcacgacg gtcgacgccg ccgtgacggt cagcgacgcc gacgacacga acctcgaggg
    20281 cggccaggtg cgcatctcga ccggcttcca gtccggcgac gacctcgtgt tcgtcaatca
    20341 gaacggcatc agcggcgtct acaacaccgg cacgggcgtg ctcacgctga ccggcacggc
    20401 ctcggtggcg aactaccaga ccgcgctgcg ctcgatccag taccgccaca cgggcgacaa
    20461 ccccaccggc ggcaagacgg tcgagttcaa ggtcaacgac ggtgatgtcg actcggcgac
    20521 cgacaccaag acgatcaaca tcacgggcgt caacgactcg gcggtgctca ccaccacggc
    20581 cgcggcgctc agctacagcg agggcagcgg cgcggtcccc gtcgacagcg gcctgacgct
    20641 cgacgatccc gagaaccacg acatcacggg cgcgacggtc aagatcaccg gcaacttcgc
    20701 gcaggccgag gacgacctcg tcttcgtcga cgccaacggg atcaccggct cgtacgacga
    20761 cgcgaccggc accctcacgc tgagcggcac ggcctccgag gccacctacc agaccgcgct
    20821 gcgctcggtc gcctaccaga acacctccga caacccgtcc acgacgaccc gcacggtcag
    20881 cttcaagacg accggtgaca cgggctcacc gagccaggga tcgaacaccg cgacgcgcga
    20941 catcaacgtg tcgccggcca acgacgcgcc ggtcgtgact ccgaccaacg gctcgacggc
    21001 gtacagcgag ggcggtgcgg cgacgaccgt cgacggtggg ctcaccgtca gcgacgccga
    21061 cgacaccaac atcgagggcg gccaggtccg gatctccagc ggcttccagt ccggcgacga
    21121 cctcgtcttc gtcaatcaga acggcatcag cggcgtctac aacaccggca cgggcgtgct
    21181 cacgctcacc ggcacggcct cggtcgcgaa ctaccagacc gcgctgcgct cgatccagtt
    21241 ccagagcacc aacgacgcgc cggtcggctc gaagacgatc gagttcaagg tcaacgacgg
    21301 cgacgtggac tcgaacgcgc cgaccaagga gatcacggtc accccggtca acgacgcccc
    21361 gacgctcaac acgagcggcg gaagcgcgtc cttcgtcgag gatggctcgg gtgcggtggc
    21421 ggtcgatccg aacatcaccc tgaccgaccc ggactcggcc aacatgagcg gcgcgaccgt
    21481 ctcgatcacc ggcaacttcg actcgagcga ggaccagctc ctcttcgcca acacggcgaa
    21541 catcaccggt aactacaact ccggcaccgg cgtgctcacg ctgaccggca ccgacaccgt
    21601 ggcgaactac caggcggcgc tgcggaccgt gcagtacaac aacacggaca cggtcgcccc
    21661 caacacggcc acacggaccg tcagcttcca ggcgacggac tcgagcgccg cggcgtcgaa
    21721 caccgcgacc cgcaacgtga cggtcgcaca gagcaacgac gcccccgtcg tcaacaccac
    21781 gggcggcgac acggcctaca ccgagggcgg cgcggctacg gcgatcgaca gcgggatcac
    21841 cgtcaccgat ccggaggaca ccaacctcga gggcggccag gtccggatct cgagcggctt
    21901 ccagtcgggc gacgacctcg tgttcgtcaa ccagaacggc atcagcggcg tctacaacac
    21961 cggcaccggc gtcctcacgc tgagcggcac cagctcgatc gccaactacc agaccgcgct
    22021 gcgctcgatc cagttccaga gcaccaacga cgcgccggcc acctcgaaga ccgtggagtt
    22081 caaggtcaac gacggcgacg tggactcgaa cctggccacc aagggcctcg ccatcacccc
    22141 cgtgaacgac gccccgacgc tcaacacgag cggcggctcg gcggcgttcg tcgaggacag
    22201 ctcgggtgcg gtgaacgtcg atccgaacct caccctgacc gaccccgact cggccaacat
    22261 gagcggcgcg acggtcgaga tcacgaccgg cttcgactcc tccgaggacc agctcgtgtt
    22321 cgtcgaccag gccggcatca ccggcgccta caacagcggc accggcgtcc tgacgctgag
    22381 cgggacggcc tcggtcgccg actaccaggc cgcgctgcgc tcggtgaagt acaacaacac
    22441 cgacacggtc gcgcccgaca cgaccacgcg gaccgtccgc ttccaggcca ccgactcgtc
    22501 cgccgcgacg tcgaacttcg cgacccgcaa cgtgacgatt gcctcggtca acgacgctcc
    22561 cgtggtggcc acctcggccg gctccacgcc ctacaccgag gacgacccgg ctacgacgat
    22621 cgacggctcg ctcacggtca ccgaccagga cgacaccaac atcgagggcg gccaggtccg
    22681 gatctcgagc ggcttccaga ccggcgacga cctggtcttc gtcaaccaga gcggcatctc
    22741 cggcgtctac aacaccggca ccggcgtcct cacgctcacc ggcacctcct cggtcgcgaa
    22801 ctaccaggcg gcgctgcgaa gcatcaagtt ccggagtacc aaccagcagc cggtcacttc
    22861 caagacggtc gagttcaagg tcaacgacgg cgacgtggac tccaacctcg ccacgaagga
    22921 gctcgcggtc acgccgacca acgacggccc gatcgtcgac gcgacgaaca ccacgctctc
    22981 ctacaccgag aacgatcccg ccacggcgat cgactccggg ctgacgatcg acgacctcga
    23041 gaacgacaac ctctcgggcg gctcggcgtc gatcacggcg aactaccagg ccggccagga
    23101 cgtcctcgcg tggaccgaca acaacctggc cgacaacatc acgctcgacg gcgtcaactc
    23161 gaacgcgcag acggtcgtcc tgaccggact cgacaccgag gccaactacg aggcggctct
    23221 gcgggcggtg acgtaccaga acgtgtccga caacccgatc accgccaacc gcacggtcac
    23281 cttctccgcc accgatcagc ccggcctgac cggctcggac acgcgcacga tctcggtcac
    23341 cgccgtcgac gacccgcccg tcgcggtcaa cgactccgcg accgtgctcg aggacgccgc
    23401 ggccacctcg gtcccggtcc tgacgaacga cacggacccc gacggcggcc cgaagaccat
    23461 cagcagcgcc accgacccgg cgaacggcac ggtcgtcctg accggcggct cgcccggcgc
    23521 gcacaccggc ctgacctacc agccggaccc gaactactgc aacgacccga gcggctcgcc
    23581 ggacaccttc agctacaccg tgaacggcgg ctcgaacgcg accgtctcga tgaccgtcac
    23641 ctgcgtcaac gacgccccgg tcgccaacaa cgacaccttc ggcggcgccg gcaacgtgaa
    23701 cgaccaggcg attggcaaca cgaccatgca ggtcgacgac acgaacgacg gcggcgacga
    23761 caacaagccc gccccgacca acccgcacac cgagatccag ggcgacatcc tcgccaacga
    23821 caccgacgtc gatggcccga cgccgcgcat cgtgcagagc gcgggctcgg acgccggtgc
    23881 gaccaacggc cagaccgccg acggtggcac ggtgaccatc gagcccgacg gtgacttcgt
    23941 ctaccagccg ccggcctcgg tcagctgcga caacggcacc gacaccttca actacaagat
    24001 ctccgaccag cagagctcgg gcgccgggcc gattccgggc accgcgatcg ggacggtcac
    24061 catcaacctc gagggctgtg tctggtacgt gaacaacaac gccccaggga acgccggcac
    24121 gagcacgcag cccttcgaca cgctcgccca ggccgagacc gcctccggcg ccaaccacac
    24181 ggtcttcgtc ttcgacggcg acaacacctc gaccggctac cagaccggct accagatgaa
    24241 ctccggcgag cggctgatcg gcgagcacga gggcctggtc gtagacgccg accagggcgg
    24301 ctcgatgtcg gccgacacgt tgcacccggc caaccccggc gcccacccga cgctgagcgg
    24361 caccaacgag gacgtcgtcg cgctcgacga cggcaacgag gtgcgcggct tcaacctcga
    24421 tccggtcggc accggcagcg gcatcgcggg cgcctccggc gacaccggcg gcggcaccat
    24481 cgacgacgtc aacatcctcg acaccgcggt cgcggggtcg gagccgctgc tcgagctcga
    24541 ctcgaccacc ggcacgttca acatctcgaa cctcgtggtg aacaaccagg ccgccgtctc
    24601 gccgccgaac acggccaccg gcgtgcgcct gaacaacgcc ggcacggtga acttcgcacc
    24661 ctcgagccag atctcgatcg gcatcaacgg cgccaagggc ctcgacgcgg ccagcaccgg
    24721 cctcggcacc agcaccttcg acgacatcac cgtcgcgggc tccggcgtcg gcggcgtgcg
    24781 gatggacacc acgaccggca ccgtcgcgct cggcgacggc accgggtccg acctgagcct
    24841 gaccacgacg tccgggtccg aggcggccct gcgcgtgaac tcgcacaccg gcggcgccct
    24901 gacggtcgac tcggccggag cggacgacct gcacgcgacc ggcggcccgg cgctcgacac
    24961 caccgccacc agcggcgcca cctacagctt cgacgacgtc gactcgacca acagcgccaa
    25021 cgacgggatc aacatcgacg gcctcggcac cggtaccttc agcgccacga gcggcgacat
    25081 cggcggctac gcgggcatcg gcttcgacct gaacgccggc agcggcgcga tcacctaccc
    25141 gggtaccttc gccaacggca gcggcaccta cgtgtccgag atcaccgggc gcagcggcgg
    25201 cgtcgtctcg ctctcgggca gcatcagcga cacgaacgac agcggcggcg gcatcaacgt
    25261 cacgggcaac acgggcggct caacggtctt ctccggcgcg accaagcagt tcaacacggg
    25321 cacggtcgac ggtgtggact tccagtcgag cgacggccac accctggtcc tgagcggcgg
    25381 cggcaccgac atcgacacga ccagcggtcg cggcgtcgac gcggagacca gcggcaccat
    25441 ccagatctcg ggcagcggca acacggtcct caccgacacc ggccgcgcca tcaacgtcgc
    25501 caacaccgac ttcgcggccg ccgacatgac cttcgactcc gtccgggtca acagcgacct
    25561 ggccggctcc ggacccgaca tcggcatccg cctcgacacc accggcaacg ccggcaacct
    25621 ggccgtgacc ggtaccggcg gcgactgcac ctccaccgcg agctgcaccg gcggcgtgat
    25681 ccacaacacg acctccgacg gcgtgcgcct gacgagcacc gccggcacct cgctgaccga
    25741 catgtacatc ggcgacagcg acggcaccgg catctacgcg ccgagcggga cgagcaacaa
    25801 caccttcaac gacctgcaca tccacaacaa cggcgacgcg gtcagcgcgc cgggggtccc
    25861 ggaggcagga atcgcgttcg ggtcgatcac cggcaccaac tcgatcaccg gaccgaactc
    25921 gtcggccggc accaccaccg tcgaggcgtc gcgcaacaac cagatcgagt ggctccaggc
    25981 ctccggcacc gggacgctga acatctccaa cacggacgtg atcaacggcg gccagggcgc
    26041 gggcaccggt tccaacgggc tctccgtgga gagccagggc accgcgaccg ccaccctcaa
    26101 cgtcggcaac ggcggtgcga gcgcggacag cctcatcgac aacaaccccg cggcggcgat
    26161 ggtcaccaac gcgaccggag cgggcgcgac gatcaactcg gacgtggaca acgtcgactt
    26221 cgacggaccc ggcagcttcg gcctctcgca cctcggccgc ggcaccacgg tctggcgcgt
    26281 caacaactcc gacttcaacg gcttcaacgt cgcgccgaac aaccagagcg cggccagcac
    26341 cgtcaccatc aacccgatcg agagcggctc gatggacggc accttcacga acaaccggat
    26401 cggcaccgcc ggcgtcaacg gctcgggcgc cctgcgcaac ttcggcctgg tgctcggcat
    26461 cgaggacggc tcggacgccg acctgaacat ctcgaacaac accatccgcg agaccgcgca
    26521 ggaggggatc ttcctctacg cccgcgactt cctgctccag ggcgggagcg tcagcaacgt
    26581 gaacctcacg ctgcgggaca acgtcgtgag cgagatcgag aacctgcctt cgccggacac
    26641 cttcacgaac ctgaacggga tcgaggtcga ggccgcgttc gactcgaacg tgtgcgccga
    26701 catcgcgaac aacgactccg agtcggtcgg ctccagctcc accacgggga cggagctgcg
    26761 cacgcgccag ctcgacgact cgatcttccg cctcgagggg ctccccgccg acagcggcaa
    26821 caacgagccg ctcgtcgagt ccttcctcgc cagcaggaac ccgctgatcc cggtcaccga
    26881 gatcgacgcg ggcatcgagc aggacgccac gcccgagggc tacaaggccc agcccggcga
    26941 ctgcccgatg ccgatcacgc ccccctgatc ggcccccgcg accgccgcgc aatatcctga
    27001 gcgcatgacc gttcaggcgc cctcggggct cgaaggcgta gtcgccttcc agacgcagat
    27061 cgcggagccc gacaaggagg gcggcgcgct ccgctaccgc ggggtcgaca tcgaggacct
    27121 ggtcggcagc gtgcctttcg cgaaggtctg ggggctcctg gccgacgact cgtacgagcc
    27181 ggggctgccg ccggcggtgc cgtacccgat cccgctgagg acaggcgaca cgcgagtcga
    27241 cgtgcaggct gggctcgcgc agatcgctcc ggcatggggc ttccagcagc ttctcgacat
    27301 ctccgacgag gaggcgcgcg accagctcgc gcgggcctcg gtgatggccc tgtcgttcgt
    27361 cgcgcagtcc gcgcgcggga tgggtcagcc gcccgtgccg gaacgggacg tcgaccaggc
    27421 ccgctcgatc ccggagcggt tcctgatccg ctggcgcggc gaggccgacc ccaatcacgt
    27481 caaggcgatc gactcctact ggatctccgc ggcggagcac ggtatgaatg cctcgacgtt
    27541 cacggcccgg atcgtggcct cgaccggggc cgacgccgcc gcggccctct cggcggccgt
    27601 gggcgccctg agcggccctc tgcacggcgg ggctccgtcg cgggtgctcc agatgctcga
    27661 cgaggtggag gcgtccggcg acgccgaggg ctgggtgcgc gacgcgctcg acaagcacga
    27721 gcggctgatg ggcttcggcc accgcgtcta ccgcgccgag gacccgcgcg cgcgggtgct
    27781 gcggcggacc gcgagcgaga tcggcgcgcc gcggctcgag gtggccgaga agctcgaggc
    27841 ggcagcgctc gcccagctcg ccgagcgcta ccccgaccgc gtgctcgcca ccaacgtcga
    27901 gttctggtcc gccgtcgtgc tcgactatgc gggcgtgccg cccgacctgt tcacgccgat
    27961 gttcgcgtgc gcgcgcgtgg ccggctggtc cgcgcacatc ctcgagcaga agcgcgaggg
    28021 tcggctgatc cggcccaccg ccgagtacgt cggccacgcg ccgcgctcgc cagacgaagt
    28081 cggctagccg ccagccggcg tcttgcggcc gcgcgcctcg cggcccgcgc cgtaacacgc
    28141 cgctcgcctg gcgcggccct cgcggcccgc gccgtaacac gtcgctcgcc tcgacctcgg
    28201 aactgggcaa agttagggtc gctatacgac gtcaacatgg cccagttgcc gatccggccc
    28261 cggcgagcgt tacgggaggg ttacgactgt gacgagtccg cgtcgggctc gtcccacagc
    28321 ggcggctggg gaggggccgg ctcggcgggg gctccgggtg gtcggcgggc gccccgcggc
    28381 gtcgcgggcg ggagcacgcg ggcgggcagg gcctgggtct cggcggtcgg ctgctcgccg
    28441 cccggcgccg ggcgaggcgg ggtggacggc gccagctcgc gcgggcccgg cggatccgag
    28501 gcgggggggc ccggcggagc cgggcccggc tcgccagcgc ggggtggagc gggcggcggc
    28561 tcgcggtcat ccgggtcggg cggcggcgcc gccggcgggc cgggctcggc cgcgcccgcg
    28621 gggaggtcgt cgggcggacg gttcgcgtgc gcgcggagcc cggcgagcag caggatcgcc
    28681 gcgcacacga gggccagcag gctctgaccc ggcgggcgat cgaggaggcg gaccacgatc
    28741 aggagcgcgg cccaggcgcc cgcggccgcg atgacggcgc cgtctccgaa cgggacgtgg
    28801 aacagcttgc cctcggcgcg cttgcgcagc aggaacagca caccggcgcc gatcagcacg
    28861 atcgccgcct ccacgaacga gaacggcccg agcgtcgaga cgatcagaag cacggcgccg
    28921 accgccgccg cacgctgatc gaagttcagt cgcttccagg tgccggcgac cgagagggtg
    28981 ggcgcctcag cgcgcgccgt ctcggggcgc gccgcctcgg gccgcgccgc ctcgggccgc
    29041 gccgcctcgg gcggcgccgc ctcggcgcgc ggcgcctcag agcggttcgc gtccgccata
    29101 gcccgagtcg aggtcgtgcc agtagcccac ctcgtcctcg ccctcctccc agcacaggta
    29161 gacctcgcgg ccgtcgcgct gcgccggaaa gtcgatcagg ccgcggtcga ggtcgcgcag
    29221 cacgatctcg ctcgcctgga gctcgcccag cgccgagcgc atatcgagga acgcctcgga
    29281 gacgtggcgg cccggctcgc cgccgccgtt catcggtgcg gcctcggcga gcgcctcgcg
    29341 cacctcctcg tcggtcagcc gcacgcgcgc cgagcgcagt cgctcgatcc gctcgagcac
    29401 ccacggcagc gcctcgtttg cctcggcgac cgtgtagtgg cgcgtcgcca tcaggacggg
    29461 gcgggcgagc cggaggccgc gcgcagccgc tcctcgagca gccgcgcgtc cgcgtcgctc
    29521 acggtgcgga gctgaccctg gaacgcgagc gtccagtgct ctcgcggcca cttgcggacg
    29581 tgctcgagct cgtccgcaag cgcctcggcg ggcacgtact cgtgctcctc cagcaccagc
    29641 tcgggctcgg tctcgaagcg ccacgggtag gcgtcgacct tcccgggctt gccgggccac
    29701 accggcgtgc gatcctcgaa catctcgccg gtcacccgca cgatcccggc gaactccttg
    29761 atgcgcgtca ggtagaagac gatccggtcg cccggctcga cctgctcggc catccggcgg
    29821 cggatctgct tcatgccgat cagcttgaac ccgcgctcgc gcgtggcggc gaagttctcg
    29881 ggcgagccgg tgaggatcca ggtcttcaat cgatcgggat gtcctcgacc tggggggcca
    29941 gctcgggctt gtcgagccgc tcgaggtacc agtcgatcag cgcgcgcacg gtcagcagca
    30001 gctcgcgctg gagcgtggtg agctgctcct ggagctcggc cggcacggcg cgccgcagcg
    30061 cctccagcac cgcgatcagc gcggacaggt cgggctgcgg cggtacgccg ttactcgact
    30121 gctgctccat gctcctcctt ctcgaacagc acgctgaggg ccccgtcctc gaaccgggcc
    30181 gagcgcggcc ggtacgtggc catcgtggcc ggcagcatga tcgtgcgctt ctggccgccc
    30241 acccggacca ccacctcgag gccgatcttc ttgaggtcga tgtcgccctt ggcggcgaac
    30301 gggaccgcca gccgcagcgt cgcgctcccg ttctcggaca cgagctcctg cgagaggtcc
    30361 tggtggagga tcgcctcggc gcgctcgtcg ccgtcgaaga cctcgtcgcc gagccggtcg
    30421 agcatcgcgc cgccgaccac ctcctgctcg aggtacggag cggtgaggat cggcacgggc
    30481 gcgaacgccg agcgcaccag ctccatctgc tcctgctgca cctcgcgcca gcccgcgaag
    30541 tacccctccc cggcctcctc cgggaagacc ctgttcacca ccaccgcgtc ggtcaggtag
    30601 ccgtagaggt tcaggtacgt gtaggtccgc atcgcctcgc ggatgaccat ccggtcgggg
    30661 ttcatcacga gccggatcga ggtggtggag cggtcgcgga ggatcccgtt catcgagccg
    30721 aggttggtca cgagccgctc cacgtcgtcg aagacgcgct cgggcggcag gttcacgtcg
    30781 agcagggtgc gcgcgaacgg gcgcgccgcc gccatcaggc ggcgctcgat cgggaacacc
    30841 ttctcgagcc accagctcgc cacgtcgggg aacgacagga gcctcagcgt ctcgccggtg
    30901 ggcgcgcagt cgacgatcac ggcgtcgaac tcgtcgctct cgtggtgggc cttgatctgg
    30961 agcagcgaga acagctcgtc catcccgggc gggacggtca gctcgtccgc ggtgatgcgg
    31021 tcgaccccgt gctcggtgag catctggccc atccagtcct gtacggcgtg ccagtggcgc
    31081 tccatctcct cctgggcctg cacctcctgg ccccagaggc cgtcgccgca gggggtgggg
    31141 gacccgccga gctcggtctc gagcgagtcc gacaggctgt gggccgggtc ggtcgagacg
    31201 atcacggtgc gcaggccggc cgccgcgcag cgacgcgcgg tggccgcggc cacgctcgtc
    31261 ttgccgacgc ctcccttgcc cgtgtagagg atcgtacggg gcggcattcg accgcccatc
    31321 gtaggtgact agccgctgcg gacggtgatc gtgcgcgtga gcaccgtgcg gttcttgcgc
    31381 aggtcgagcg tgtcgatctc gagcgtgtac ttgcccgccg tctgcggctg ccacgtgaac
    31441 gagcgccggc cgcggttgaa cttgatctgg ctgcggtaga cctgcgcgcc cgcggcgtcc
    31501 ttgatcgtcg ccgtcacgca cgagaccttg ttcagcgtga acgtcagggc ggtgcgccgc
    31561 ccggtccgcg cgctcgacgg cccggcgtac gcgaccttcg gcgcggtctt cttgtaggcg
    31621 ttgaagcgct cggccttctg gcagtagacg ctcgccttcg tgcgcgtgca gaggttctcg
    31681 aggaagtcgg tcaccacgtt gtggtagttg aggttcgact ccgcgccgcc ctggctgtac
    31741 agcgaccagg cgccggtgtc gtaacgcggc acctcgcgcc gcgccgatcg gtcgcccgcc
    31801 cggaacagcc gcatcgcggc gtcgtccttc gcggtcttcg cgtagtcgta gatcccggtg
    31861 atcgtctgga ggaaggcgtt cagcaccctc agcccgggcg agaacgagta gagcacgtag
    31921 tggctgccgc ctccctcgcc gcgcacgcgc acgccggtgg gcgtggactt ctcgaagatc
    31981 ccgaccgccg atttggccac gcgcaggtag gatggctcgg cgagcaggat cgagccgcgc
    32041 gcgagtgcct gcatcgccgt cccctgcgcc atgccgctca cccacggcgg ggagccgccg
    32101 ccgaacgcga accagtactc ccaggtggtg aagccgccgc ggctcgagcg gatcgcgacc
    32161 agctcgtcga gcagcttgcg cagcgcgacc gggtcgcagc gcgcctcctt gcggcggcag
    32221 gccgtgtaga tcccgttggc cttgccgaag ttcgcgagcg gctggatctg gagtccgttg
    32281 ccggggtagt actggtagac gatctcgcta ccggagaaca tgatccgcga gcccgcccgg
    32341 ccgctcgacg cgggctggca gggctcctcg ggcacgtcct cgcgggcggg gaactcaggg
    32401 tcgccgcccc agaactcgtc gttgcggtcg agctggagga agagcgcggg catccggctg
    32461 cccgtgagcc ggtcggcggc cgcaatccgc tcgagctggg agatcacgct cgagagctcg
    32521 ctgcggtagc tgccgctgag cttcgcgcgc gtggcgcgcg cctcgcggta gatgcgcttg
    32581 tactcggccg ccgccggggc gtcgatctgc ttgcgcgagg aggcgcggtc gatcgcctgg
    32641 ttcacgctgg tggccgacgc gcgcagggcc ggctgcgcgc aggggccggg cggggccggc
    32701 gggtcgctga gtgcgggcgg cggcagggcc gggtcgtcgc tctgctcgag ccggtcgccg
    32761 tcccagtgca ggacggggct cgcgctcgcc ggggccgcga gggcgagcgc caaaaggcac
    32821 agtgtgcagg tgaatctgac cgcagtcaca gcttgtagtc tcgatcgctt gcgcgcgttc
    32881 gtacttctag cagccgtggc agcgctcctc gccggctgca acggcggtgg cagcgagggc
    32941 gacgcgcgcg agatcctcca gaagggcttc ggcgggtcga tcggcacggc ccgcctcacg
    33001 gtggacctca ccgcgaagct ggagggcatc ccgcagcaga agggtccggt gaggatcgag
    33061 ctcaacggcc cgtaccgctc cggcccgccc gggcgcctgc ccgacgccga cctcgacatg
    33121 gcgatctccg ggggagggca gacgttctcg ctcggcctca tctcggcgga cgagcgcgcg
    33181 ttcgtggagt tcggggggcg cgcgtacgag gtgagcgccg cgacggtcgc gcgcctgaac
    33241 ggcggcgact ccccggctcc gggcggcggg ccgggattgc gcggcgcgct gcgccagcgc
    33301 ttcggcgtcg acccgctcga ctggatcgcc gacccgcgcg acgagggcga ggcgacggtc
    33361 ggcggggacg cgacgcgcca cgtgcgcggg gacgttgacg tgaagaaggc gctcggcgac
    33421 ctgaacaggg tcgtcgcgcg cacagcggca ccgggcagcc agccgcccac cctggacgac
    33481 gcgcaggtcg agcgggtctc cgagctggtc gacgacccga agctcgacgt ctacgtcggc
    33541 cgcgacgacg gcaagctcag gcgcttctcg ctcgacctcg ccttcgaggt gcccgagcgc
    33601 gaccgccgcc gcctgaacgg cctgcgcagc ggcacggtca cgctcgacgt cgagctgaac
    33661 gaggtggggc agccgcagcg cgtcgaggcg cccacctcgt cgcgcccgta caaggagctg
    33721 aacgacctgc tcggcggccg cgggatcatc ggcctcctgt tcggagcgac caccggcgcg
    33781 ccgcagcccg gcggcccgaa ctcgcccacg cagcagcagc tcgaggccta ccgcgagtgc
    33841 atcgaccagg ccaagccgag cgatacggcc acgatcgagc gctgcaacga gctgctgcgc
    33901 taggtccgac tacgccgcgg cgggctcgac gtcgaagttg cgcacgtaga gctcctgcac
    33961 gcgcgccagc tcgtcgtcgg tgaggtccgg cgcgtctgcg gcggcggcga actcctcgag
    34021 ctcggccagc gtgccgatcg tcgggataac gcaggccatc tgcggccggg cgaggatgaa
    34081 cttgagcgcc gcctgggtca tcgaccgctc gccctcgatc acgagaaagc gcagccggtc
    34141 gatcttctgg agcccgtcca cgagccactg gcgcgggcgg tggcggcggt ggtcgccggg
    34201 gccgaactcg gtgtcgagcg tgtacttgtc ctcgagcagg ccgctcgagg tgggcacgcg
    34261 ggccatgatg ccggcgccgc tgcgctccgc ggcggcgagg aagtccgcgc ccggatcctg
    34321 ctcgagcagg ttgtagaccg tctgcaacgc cgcaatgccg cgccgctcga gcgcgagcac
    34381 gccctcgtca cgccagccga tcgccggccc gagcgcgatg ccgtagtggc ggatcttgcc
    34441 ctcgtcgcgc agcgcgtcga gcacggcgaa cagctcgtcg ttctcgatcg cgtccatgcg
    34501 cgggttgtgg agctggtaga ggtcgacgta atcggtgccg agccgccgca ggctgccctc
    34561 gagcgactcg cgcacgtggg cgggcgacca gtcgtggggg cgctcgccct ccgagtgctc
    34621 ctgccggccc gagtcgagca cgtagccgaa cttcgtcgag atctggatcg agtcgcggtc
    34681 ggcgcccgcg agcgctcggc cgaccatctc ctcgttcttg ccctgcccgt agacgttgcc
    34741 ggtgtcgaag aacgtcacgc cgagctcgag cgcgcgcccg atcagccggt cggcgtcgtc
    34801 ggtctcgccc caccaggtcg agccgagcgc ccacgtcccg aacgcgatct cggacgcggt
    34861 gaggtcggtg gtgccgaagt tgcgctgcct catctccgag ccgcagtcta ggtcatccca
    34921 attgccgcct agggcgtatc tggcgcatgg gcaaggacaa gctgagcgga cggcgcgtgg
    34981 tgataacggg cgccagcagc ggcatcgggc tcgccgcggt gccgcgcttc gcggccgcgg
    35041 gtgcgcggct cgcgctgatc gcacgtgacg aggagcgtct ggcggaggcg gtggacgcgg
    35101 cgggcccggc cgcccgcgcc ttctcggccg acctcggcga gggtgactcc gcggatgcgg
    35161 cgatcgcggc cgccgcccgc tggctcggcg ggatcgacgt gctcgtgctg aacgccgccg
    35221 agtcctcgta cggaccgttc cgcgacacgc cggcggagga cttcgagcgc acgatcgaat
    35281 cgaccttcgg cacgggcgtc aacgccgtgc gcgccgccct cccgcacctc gacgagtcgg
    35341 agggctccgt ggtcgcaaac ctgtcggtgc tcagccgctt cccggtgccg atgttctccg
    35401 gctacgtggc cgccaagcac gcgctgcgcg gcttcctcgg cgcgctccgc atcgagctcg
    35461 agcgcgacgg cagcggcgtg caggtgtcga tcgttcaccc cggccatgtg gacacgccgt
    35521 tctggggccg ggtcaacagc gtgaccggcc ggctgccgcg ccttcccccg ctcgcctacg
    35581 acgccgacag ggtcgccgcc gccctggtcg atgcggccga gcgtccgcgt cgcgagcaca
    35641 ccgtcggggc gctcgccagg ctccaggtcg tggtcgccgc ggtggcgatg ccgctcgtgg
    35701 atctgagcgg gagggcgctc gggcgctggc tcgagagcgg ggagcaggcc gccgcgtagg
    35761 ctcctcggtg atggtcgccg acatcgaatc gatcgcgatc ctggtcgcgg cgctcctcgt
    35821 attcctctgg ctcgacctcc acttcttcgc gcgcgggcgc gagccgacgt tccgcgaggg
    35881 cgccatctgg tcgatcggct ggctcgtcgt gagcctgctc gccgggttcg tggtcgcggc
    35941 ggtgcgcgac agcgacgacg ccgtcctcta caccaccgtc tacctgatcg agcgctcgct
    36001 ctcgctcgac aacctgttcg tcttcatcct gctgttctcg tacttcgcgg tgccccagga
    36061 gcagcgcggg cggctcctgt tctggggcat cagcttcgcc ctcgtgctgc gcggcctggc
    36121 gatcctcggc ggcgtcgcgc tcatcgagca gttccacttc gtgatctacg tgctgggcgt
    36181 ggggctgctc gtgctcgcgt accggatcct gcgcggcgtc gccgagaacg tcgatcccga
    36241 caagaacctg atggtgcgcg tggtgcggcg cttcttcccg gtgaccgggg agttccacgg
    36301 gcggcactgg ttcgcgaggg tccaggacaa gcgccacgtc accccgctgt tcctctgcct
    36361 cgtggcgatg gtcttcgccg acatcgcctt cgccatcgac tcgatcccgg cggccttcgc
    36421 gatcaccacc gacggcttcc tgatctggat gggcaacgtc ttcgcgctgc tcggcctgcg
    36481 cgcgctgttc gtgctggtcc aggcgctgat gaagcgcttc cgctacctcg acgagacgat
    36541 cgcgatcgtg ctcgcgctgg tcggcctgaa gctgctgatc gaggacatcg tgaagatcag
    36601 ccccgaggcg agcctcgccg gcgtggcgct ggcgttcgcg gcgggaatca gcgcgtcgct
    36661 gatcgccgac aagcgcgacc ccgagggggc cccgcccgag cgcggcgagc cgctgctcga
    36721 ctcgacggcg tcaggcgtag aagaagacga gtccgaagac cgcgtacaga gcgagtagct
    36781 gcacgccctc gaaccagttc gactcgccgt cctgcgtgac gaagttggcg atgagcaccg
    36841 cgaggaccag ggcgcccagc tcgtagccgt tgaacacgag cgccatcggg ttctcgccga
    36901 ccacgaacga gacgagcacc aggagcggtg cgacgaacag cgcgatctgc gcgctcgacc
    36961 cgatcgcgat gttcacggcg aggtccatct tgttcttggc cgccaccagc accgcgaccc
    37021 agtgctcggc cgcgttgccc acgatggcga cgacgaacac gcccacgaag aactgagaca
    37081 ggccgatgtc ctccgaggcg tcggagatcg agccgacgag gatctcgctc atcactccga
    37141 cggccacggc ggccacggcg agcatcccga gcgacttgcg caccgaccag ccgagcgtgt
    37201 cctcgtgctc cccctcggtg tacgggttga agagctcgcg gtgggtcttc aggctgaaga
    37261 ccagccccgc cacgtaggtc aggatcagca cgatcgcgac gccgaacgag aggtgctcga
    37321 ggtcggtgcc gaagtcgacc cgctcctccc cgacggccgg caggccgccg ccgtggatca
    37381 gctgccagac ggcgggcagg atcagcgcgg cgagcgccag cagcagcatc gccgactgcg
    37441 cgtgcgcggc ggtggcgttg aaggtctgct tctcgcggtt ccagccgccg accagcatcg
    37501 cggcgccgag cacgagcagc acgttgccga cgacggagcc gacgatcgag gccttcacga
    37561 cctcctgaag tccctcgagc agcgcgaaga acgcgatgat cagctcgggc gcgttgccga
    37621 acgtgacgtt cagcaggccg ccgatgccgg ggccggactt ggcggcgagc tcctccgtgg
    37681 ccatgcccat cagcgccgcg gtggggatca cgcccagcgc cgcggcggag aagatcagga
    37741 ccgggttcgc gtgcgcgatc tcgagcgcga tcgcgaccgg tatgaatgct gcgaggagaa
    37801 agacggggcg gcgcacgcgg gcggagagcc tacgaagcgg ccggggctac tcgatctcct
    37861 cggctgcttc cggcttgggg tcctcgtcgt cctctacggc gtcgaggcgc tcctgctcgt
    37921 cctcgtcgtc gagcgtgacg tcgcggccgt caccgccggc ctggcgcgcc tggaagccct
    37981 cgaccatctc gtcgcggcgg gcctcgtcga ccggcaggtc gtcggagatc agcacccagt
    38041 cggcgccctc gaagtcctcg aggttgaaga tctcgttgtc gagcgacgcg gagtcgtcca
    38101 cgacgaggag cacctcggtc tgagggttga agtaggtgcc cggacggacg attagctcgt
    38161 cgagctcgaa ctggcggtcg ctggccatcg gtccgtatct atacccgaaa agcgcgcggc
    38221 gaacaggccg gcgtgcgctc aggcgcctat gtcgcggagc tggcggtcca cctcggcctc
    38281 cacgtcgagc ggctcctggc cgcgccgctg gcggcgttcg ttgcgcgcca cgacgagctg
    38341 gcgcacctcg tcgcggagcg ccgcgtccgc gccggccggg cgcgtgagcg ccgctatctc
    38401 ggcgtcgatg tcgagcgcgt cctcgccgcg cgcctcgcgc cgcgcggact tcgcctcgac
    38461 catctggcgg atctcggcgg cggcctcggc gtccgcggcc gcgctgccgg gcgcggggcc
    38521 gggtgtgcga tccggctggt cgagcgcgaa gtcgcccttg ccgatccggt cgtagacgcc
    38581 gccggctccg acgaaggccg cgaaggccgc gagcgtcgcc aggatcacga tcacccagag
    38641 gaccgtctcg aaggcgctct ccacacctac agtgtggacg atcatgtggc gccgtctacg
    38701 ccgccggacg atggccccga gccggccggc gatctacccc gaccagatcg ggctcgtgat
    38761 cctcaccgtc gtcgcgatcg ggctcgggat ttacctgatg gtccgggtgc tggcgggctg
    38821 aggcgctacc attcgcagtc attgactgac gagtcaatcg gactcttttg aaccaccgcc
    38881 cgcggccgtc cgcggcgcca cacaaggagc acgagtggtc gacctgacgc tgacagacga
    38941 gcagaaggat ctgcgcgaga tggcccacaa cttcgcggcc aacgagatcc gcaaggttgc
    39001 ctgggagtac gacaaggaag gcacctggcc gcaggacatc atcgacaagg cctgggagct
    39061 cggcctgatg aacagccacc tgccggagga gtacggcggg gccggcctcg acttcatgag
    39121 cggaacgctc atcgaggagg agctcggctg gggctgctcg ggcatcggca cgtcgctcac
    39181 ctgcaacggc ctcgccacca cgccgatcat gctcggcggc tccgaggaga ccaagaagaa
    39241 gtacctcggc atgctctccg aggagccgaa gctcgcgtcg ttctgcctca ccgagccgga
    39301 tgccggctcc gacgtctcgg gcatgcgcac caccgccgtg aagatgggcg acgagtacgt
    39361 catcaacggc tcgaagtgct tcatcaccaa cggcagctac gccaactact acacggtgta
    39421 cgcgaagacc gacaaggacg cgggccacaa gggcatctcc gcgttcgtgg tcgaccgcga
    39481 cctcgacggc gtgaccgtcg acaagaagga ggacaagctc ggacagcgcg cctccaacac
    39541 cgcgacgatc tcgttcaacg acgtccggat cccggccgag aacctgctcg gcgaggagaa
    39601 ccgcggcttc aagctcgcga tgatgacgct cgaccgcacg cgccccggcg tggcggcgat
    39661 ggcggtcggc atcgcgcgcg cggcgttcga gttcgcgacc gagtactcga aggagcgcgt
    39721 ccagttcggc gtgccgatcg ccatgcacca ggcgatccag ttcatgatcg ccgacatggc
    39781 gaccaaggtc gaggcggccc gcctgctcac ctggcagtcg gcctcgctgc tcgaccaggg
    39841 caagcgcaac acgctggcca gctcgcacgc gaagcgcttc gcggccgact ccgcgatgga
    39901 ggtcacgacc gacgccgtgc aggtctacgg cgggtacggc ttcatcaagg agtacccggt
    39961 cgagaagctg atgcgcgacg cgaagatcat gcagctctac gagggcacgt cgcagatcca
    40021 gcggctcgtc atcgcgcgcg aggtcctgct cccgcggcag gtctcggagc cggcggcggc
    40081 caccgcctag gcagcccgcc gacaagcgag ggattctcga gggccgtcct gcggggcggc
    40141 cctcgtcgtt ccggtacgat ccttcgcgct ggggcaatct ttttcggtac ggggggtcac
    40201 cgaatgaagc gtcttgttct gttcgccgtc gtgggcgcgc tgctggtcgc gccggccgcg
    40261 gacgcgaagg agtacctgtt cgtggggagc gacggcacgc gctgcaccgt cagcaccgcg
    40321 cattccacgg cgccggaggc gggcgggacg ttctcggccg acatctcgtc gaccgcgtcg
    40381 tgcagccgtc cgctgttcgg cgccttcatc cgcgcatacg tcggctcggc cgacgacccg
    40441 ttcggctacg aggccggcgg gttcagcgac agctgcaacc ccctgtaccc gtacggcacc
    40501 ggctgcgaca gggcggtcgt gacggccggc ggcacgcggg gcggacttgc gcccgggtcg
    40561 tacgtgcatg ggacgctggt cacgctcacg ctcgcccagg caccgccgcc atcgtgggtg
    40621 gtggtgccgg tgcacgagga ccggccggag agcatcagct tcgtgggcac gtgccggccc
    40681 gtctatgcgg acacgcgctg ccatttccgc gagccgttca cggcgggcta ggccggcagc
    40741 actctccttg cggaagcccc ctccggggcg gcttccggcg tttcggagcc gctcgttgtg
    40801 actcacccct ttagcggtca accgtcttga ctaggtagtc gcgggtcgct tagggtggct
    40861 cgacgcccgc gcgagtctgg aacgcggtcc gaagatcgat acgggggaga ggctgtggcg
    40921 agttacgacc aggtgaaggc ggcgctgacg ctcatgcacg acgaattgag cgcgaagtcg
    40981 agcgccgtat tggccgtggg ggtgggcttc gcggacgaga acgcgacccg gcgcgagccc
    41041 ggcaccgact gcgtgatcgt cgtcatcctc gacgagaacg gggacgatcg ggccgaagcg
    41101 gccttcccct cgagcgtgac gctcacggcc gacaagctgc gctcggccgc cgcggacgac
    41161 gagatcgacg gcgatgtcga gatcccggtg cgcatcgagc gcgaggacat ccagccgctc
    41221 atagtcaagg agcaggggga gccggtcgtc gagccgctgg acgatccggc ggcccgtgga
    41281 gcgggcgcat tcctcctcgg cggggacccg atctacacca gcgcgggcga ttggatcggc
    41341 accggcggct gcgtactgcg tatctacaac acgctctgcc tggtctcgaa cgcgcacgtg
    41401 ctcagcgaga tgggcggcgt gggcggcagg gttcgcgacc atcgcggccg cgtcgatttc
    41461 gcgaccgtca acgagctccg aaccgcacct gacagcgcgt gggctcagct cgactccgac
    41521 gccggttacc gaaacgacat cccgtacatc ggccagatcc gctgggtcgg cgggccgatg
    41581 cagctcggct ggcacgggcg aaagtacggc tcctcgacgg ggtggagcga cttccacttc
    41641 cgcttcgagg gcaccaccgc cgggggcacg ccggccgtcg gtggctggtg ggagggacag
    41701 ccgcacggcg ggaagccgac cgtcggcaac ggcgactcgg gatcggtcgt ggtcggggac
    41761 gggggcgagg tgatgggcct cgtattcgcc ggctcggaga aggtggggac ctcgccgggc
    41821 ggcagctcgt tccggaagat ctggtgtgtc tcgaacatgt ggacggcctt ccccgggccc
    41881 gaggtgccgg cctggtgaac gcggccacgg gcggcagtga ggagaacccg ccggacccgc
    41941 ggctcgagat ccacccgcgg atccgcaccg cgctgcgcgg gatgggccat tccattttcc
    42001 cattcaacgc gttcgacgag ttcggggagc tcaccctcac tccgctgaag atcccggcgc
    42061 agtacacgga gctggcgaag caggtcgccc agtcgcccgt gttcgaggcg gtgtacacgc
    42121 acatctcggt gctcgaggag gagccggact ggcgctgcta ctcgctcggg ctcagcttca
    42181 cgcccgacta cgggatatcg atcatcccgg tgaagtcgct cgacagcctg aacgtctcgc
    42241 gtgaggaagc gccgcgcgtg gccggggagt gggcgaccag cctcaacgag ctcatcctcg
    42301 agaacgtcca gcaggtcgcc ggcgcggact acgtgctccc ggaccagatc gcaaaggcgg
    42361 tgaaggaact gctgccgatc gtgcggaagc tgctccccga taagccaggc ggggggcagg
    42421 ggccggggcc ggggacggag ccggggcctg ggcctgggcc ggagccggag cctgggccgg
    42481 agccgggccc ggagccgatc gtgccgctgg acctcctgga ggcgcccggg cagcaccacg
    42541 ccggcgtgcc cgccctcgag gcggccgacg cagccctcgc ggcggcgagc ccgtggtcac
    42601 ccgtgctggg cctcgagttg gcggcggccg tcctgagcgc ggcggcgatg ctgaggaaga
    42661 agcagagcgt gacggccacg atcgtctggt acccgcccga cagctgggag gggttcgacg
    42721 aggcggagtg ggatcactac cgcaagcacc tcaagccgat ctcataccgc ggccggcgca
    42781 aggtggtcca cgcctgctgg aacccgttca gccgcaaggt gctcgcgttc ggccccgacg
    42841 acctctggaa cagcgacaac ccgcacgtgc cgaagcgccc gtcgcaggtc cagcgcgcgt
    42901 tctacccgat cggctgagcg ccgggctcag acgccggcga acagcgccag gtcgagcgcc
    42961 agcccggcga ccgcccacat cgtcgccccg gggagctcgt cgcgcctcac cgccctgacg
    43021 aacgccccga gcgacaggag cacgccgacg accgcgacga ccgcccccgc gtcgagcccg
    43081 ccggccccac cgaccaccca ggcccccacg gcaacggcga acgccaggaa gcccagcgag
    43141 cgcatcgact cctcgatgcc cttcgacttc atgccgaggc cactgccagg acgaccgtga
    43201 gtccgaccag ggcgaatgcg aagacggcca tgtagcgcgg agccactccg tcgtcttcgt
    43261 ggtcgcgcct gaacgtcgcg taggcgacga gggccgccac ggcccccagc ccaagccccc
    43321 ccagcggctg accgtccgcc gcgacgatcg ccgccattgc ggcgaagacg atcgcggcga
    43381 ggccataggc gtaacccatc cgcgcatcct aatcgctaat gggagccgaa gcgatgaagc
    43441 ggctgcgcgg ccccccgcgg ctcaggacga ggcgcgatcc gccgcgctcg ggcacacggg
    43501 ggtgcccgcg gccgagcgga ccgcgtcgcg gatcgccgcg gccgtgacgt cggcggcgag
    43561 cgcggagatc gcgaacggtg cggcctcgac atcgcccgac gcgaggcagt agacgaggtc
    43621 gccgtcgtgc gcggtcgcgc tcggctgcac ggcgcggccg acgcccgcgc tggccgcgcg
    43681 cgccacgagc cacgccgcgc gcttgtcgag ctccgcgtcg gtcatgaggc atacgagcgt
    43741 ggtcgactcg cgcgcgctcg ccggcggccc gaagccctcc ttcagcgcgt cgacggttcg
    43801 cacgtagccg ccgtcgcgcc acgcaccggc gagcaccgcg ccgtcctctg cgatcacctc
    43861 gcccacggcg ttcaccaccg cgagcgcggt cacccgcgcg ccgccgatcg agtcggaagc
    43921 gaggccgagc ccgcccttcg tccacgcctc gtggccgagc accttgccca cggccgcccc
    43981 ggtgcccgcc ccgacggagc cgcgctcgac ctccccggcg cgcgccgcct cgcaggctgc
    44041 gcgccccgca tcgggccccg gccgcgcctt cgggtcgccg agcgtgaggt cgtagacgat
    44101 tgcgcccgcc accagcggga ccaggccggc cggggtcgag tagccggcgc cgcgctcctc
    44161 gagccaccgc accacgccgt cggcggcggc gagaccgaag gcactgccgc cggcgaagca
    44221 gaccgcctgg actccgggcg cgttggccgc gggcgagagg agctcgctct cacgggtgcc
    44281 cggtccgccg ccgcgcacct ccccggcggc caccgtcccc tccggcgcca ggaccaccgt
    44341 gcagcccgtg gcgccgtcag agtcgctcca gtggcccacc ctgaatccat ccggcagcat
    44401 ggggtcgcac ggtacatggc acgattgggg tgccgatggc cgcacctcgc aagccccgcc
    44461 ctgagccccc gaagaagcgc acgcaggcga cgcgcgaggc gatcaagcgg acgacgcgcg
    44521 tcgcgagcgc gcgcgacgcc gcccgcacgc tcgaggggcc ggcggccgag cgccagccgc
    44581 gcgggggcga ccgcgacccg ttcaccggcg agctgcgcaa gcgcggcatc ctcccgatgc
    44641 tcgtcctgca cttcatgagc gagggcccgt cgtacggcaa ccagctgatc gagcggatat
    44701 ccgcggtgac cgtgggcgtg ctgcacgtga acccgaacac gatgtacccc ctgctgcgcc
    44761 agctcgaggc gaaggggctg atcgagggcc gctgggagca tcccgagcgc cgcagccggc
    44821 gctactactc gctgaccgtg gacggccggc gcgagtacaa gcggctcgtc aaggacgtgc
    44881 ggccgttcct cgactcgatc gcgcgctcca tccacgagat cgtgcgcgag gtctacgact
    44941 gatggggacg gtccgcgagg cgatctccgt caggctcgac ccgcagcgcg ccttccggct
    45001 ctggaccgac gtgcgccgct ggcagacctt cgtggacggc ttcgcgcgcg tgaagcgcct
    45061 cgacgacgag tggccgggag cgggcgccaa gctcgtgtgg gagtcgacgc cggaggggcg
    45121 cggcacggtc accgaacgcg tgaccgaggc ggaggccggc agcaggctcg tcacgcacgt
    45181 gttcgaggag cgctgcgccg gcacgcagac cgtgaccttc gagcccgacc cggagggcgc
    45241 cgtcgcgcag atcgagtacg agtacgagct cgcgagagga ggccccctcc gcgggatcac
    45301 cgacgtgctg ttcatacgcc gcgcgcagtc ggacgcgctg gtccgcaccc tgcggcgttt
    45361 cgcgacggag gccgcggagg aagcggagtt gtagccttcc ccggccgcct acaggaggga
    45421 catcgaagca tgtacgtgtt caaagccgcc gtcgtcggcg ccggcacgat gggaggagag
    45481 atcgcgcagg tcgtcgccag cgcgggcctc caggtagtgc tcaaggacgt gaagcaggag
    45541 ttcgtggacg ccggcctcga gaaggcccgc ggcgtgacca cgggccagat cggcaagctc
    45601 gtcgagaagg gcaagatcac ccaggaccag gccgaccagc agatcgagga gatcgtcggc
    45661 cgcatcaccg ggacgctcga ctacgacggg ttcggcgacg tcgacttcgt gatcgaggcc
    45721 gtgccggaga agatggagat caagcagtcc gtgttcgcgg agctcgacgc cgtcacgccc
    45781 ggccacgcga tcctcagctc gaacacctcg tccctctcga tcagcgagat ggccgaggcc
    45841 acgcagcggc ccgacaaggt cgtcggcttc cacttcttct ggcccgcgtc gatgatgcgg
    45901 ctgatcgagg tgatcgaggg cgacgagacc tccgaggaga cgatgcaggc gaccgcgaac
    45961 ttcgcccagc agatccgcaa gaccgcggtc cgctgcgctg aggtgcccgg gttcgtcgtc
    46021 aaccgcatcc tcaactcggc ggcgtcggag atctggcgcg tgaccgacga ggaggggatc
    46081 gacccgcagg aggtcgacaa ggtcatccag gagtcgaaga cgatcccgat gggcccgtac
    46141 ttcctcgccg acatggtcgg gctcgacgtg acgctgcaca ccgccgagca catcgctgag
    46201 agctacggcg atcgcttcta cgtctcggag cggctcaagg agctggtcgg ggccggcgac
    46261 ctcggacaga agaccggaaa gggtttctat gagcacggct agcaccgaat cgcagtccct
    46321 ggggacgctg gtcgagcgct tcggcctgaa gaccctcgtc gagtcgtgcc tcgtcctcga
    46381 ggagggcgtg gcgtcgatga aggacatcga catcggaatg atgatgggcg cggggatcct
    46441 ccccggcccg ttccagcgcg ccgacgcgca gggcctcgac gaggttctgg ccgcgctcga
    46501 gcgcgcgcag gccgagtggg gcgagcactt cgcgccgccg ctgatcctgc ggcgcctggt
    46561 gagccagggc cggctcggtc agaagaccgg ccaggggttc ttcccctacc cgcagccgga
    46621 cgccgactcg agctacgaga cgctcgcgat cgagacgcgc ggcgaggtca cgatcgtgtg
    46681 gctcaaccgc ccgccggcga acccgctctc gccgcaggtg atccgcgagc tcgcggacgc
    46741 gtggggcaag ctcgacggca aggcgcgcgc ggtcgtgatc gcgtcgtcaa acccgttcac
    46801 gttctccgcg ggggcggaca tcaaggagtt cacgaagatg aaccccgacg ccgagggacg
    46861 cgagctgatc gagtcgggcc atggcctgat gaccagcatg gagaagtcct cgacggtcac
    46921 catcgcggcg atcaactcgc tcgcgttcgg cggtggctgc gagctggcga tgtcgtgcga
    46981 cttccgcatc gccgcggagt cggcgtcgtt cgcccagccc gagatcaacc tggggatcat
    47041 ccccggcttc ggcggcaccc agcggctgcc gcggctggtg ggggagggca aggccctcga
    47101 aatgaacctc accggcgacg ccatcggtgc gtacgaggcg ttccggtacg ggctggccac
    47161 ccaggtcgtg ccggaccacg agctgttcga caccgcgctc gcgtgggctc gcaagctcag
    47221 cgagaaggcg ccgatcgcgg tcgagcagat caagagcgtg tccgccatgg gcgagctcgg
    47281 cgagggcctc gaggccgagg cgcacgggtt cgccaaggcg ttcgggtccg aggacgccaa
    47341 ggagggcatc ggcgccttcc tcggcaagcg gacgccgagg ttccagggca agtaggtggc
    47401 gccggccgtc ggtacgagcg cggcggtgcg cctcgcggag ctcctccgcg gggccgagcg
    47461 cgccgtcgtg ctcaccggcg ccggggtctc ggtgccctcg ggcatccccg acttccggac
    47521 gcccggcaag ggcatctggg agaaggtcaa cccgatggag gtcgcgcaca tcgacgcctt
    47581 ccggcgcagc ccggaccgct tctggcagtt ctactcggac cgcttcgcct cgctcgtgga
    47641 caagcggccg aatcccgcgc acgaggcaat cgccgagctc gagcttcgcg gcatcgtgcg
    47701 cggggtgatc acgcagaacg tggatcgcct gcaccggatg gccgggagcc ggaaggtgat
    47761 cgagatgcac ggatcgatcg agcactgcgt ctgcctggaa tgcggcgggc gggtggcgct
    47821 cgacgacgtg atcgaaatcc tcgcgcggca cccgggcgcc ccggagtgca cggcctgcat
    47881 ccacccactg aagcccgacg tggtgctgtt cggcgagatg ctgccggccg cggcgatggc
    47941 cgaggcccac gcgctcgccg cgcaggcgga cctgatggtc tgcgtgggct cgtcgctcga
    48001 ggtgttcccg gtggcctcac tcccgggcct gaccttggag tccggcgggc gcctggccct
    48061 cgtcacccag ggcccgaccc cgtacgacgg cgacgccgag ctgaagctca ccggcgacgt
    48121 ggcggacgag ctccaggccg tgttgtccgc tctgtagcgg cgcaatgggc cccgggaagg
    48181 gggcgaattt cgcgacgttc ctgacttatg ggtcgtatgg acccaaaagt caggaatgtc
    48241 gaacgaaaac tcgctcggat cgcgtcacgg tcgtggggtg tcgtgaccgt ccgggagctc
    48301 ctaagtgctg gtgtttccgc gcaggagatc aagcggcgcg tggaaaaggg tctcttgatc
    48361 cgtgagcacc gtggcgtcta ccgcgtcggg caccgcgctc cgagcgtcga ggccacttac
    48421 atggcggcgg tcaaggcctg cggggagggg gcggtgctct ccggacggtc cgccgcctac
    48481 ctccagggcc tcctgcgtaa atgcgatccg cccgcgccgg aggtgacggc cccgaccgag
    48541 cggaacgtga agggcgtgag aacgcgtcgc cggcacctcg atccgcgcga cacggccaag
    48601 ctccacggca tcccgatcac cacggtcccc tgcacgctgg tcgacctcgc ggcggaactc
    48661 agcctcgacg acctcgcccg cgcgtgccac gaggccggcg tccgctaccg caccacccct
    48721 cggcaggtcg aggccgtgct ggcacgcagg ccgaacgcgc cgggcgggag gaacctgaga
    48781 cgcatcctgc acggcgaagc cagggtcacc ctgagcaagc tggaacgccg gttcctgacc
    48841 ctattaagag atgccgttct gcccttgccg gaaaccaacc gtccagccgg cggccggcgc
    48901 gtcgactgcc gctggccgga gcacaggctc acggtcgagc tggacagcta cacctaccac
    48961 ggctcgcgcc acgcctggga gcaggaccgc cggcgcgagc gcgcggcgta cgcgcgcggc
    49021 gaccagttcc gccgctacac ctacggcgat gccttcgagg ggccggcgcc gatgatgcgc
    49081 gagctgcgtg cgctgctcgg ctagtcgtgc gacgcggcca gcgcctcgtg gcgcgggtcg
    49141 gtgccgccca tctcgacgcc gttgacccac acgcgcccac ccgtgcgcgc gggctcgccg
    49201 ccgaaccgcg cagggagggg agcgcggtcg atccggcggc gagccgcgat cgcgcgggag
    49261 acgcgcctgc tgcgcctgtc gtggatctcg gtgactgcct tcctgctcac gtcgtccatc
    49321 ttggtctccc gtccgctttg tgcctatggc cgccgggcca aacttccggc tccctgtctc
    49381 gaccaacggt cgtgacggcc aaaggttcgc cgcatactgg ccggatggcg taaaccagcg
    49441 gtgaagatcc ggctcaggtg ttcgtcacat gaccaagacg gcgccggcgc agtgccgtct
    49501 ccatccggcc gaccgccgcg gccaccgcag cgctcagttc ggtgccgggc gaacccgcga
    49561 gcgcctcgtt ggcggcgcgc ccgaccggct cgcggtcggc ggcgaggtcg cccaccgcgt
    49621 cgccccccag ctccgccagc agcgcctcca gggcgatgcg ggcctggagg gcggcctcac
    49681 ggggccgggc ggcgtcgagg tccgcacgcg cgcgcaggac gagctcctcg cacgccagca
    49741 gctcgtcgcg cccgcccagg atggccgcga gccgctcgtc gggcgcgagc gccgcggccc
    49801 ggcggcgcac cttcgacgtg tcgtcctcga gcggcagctc gtacgcctcg gcgaagtgcc
    49861 cgtcggccac ctgctccccc gagccgtatc cgacccgcac cacgttggcg cccgagggag
    49921 ccacgtcgcg cgcggaagga tccagcgccg ccgcccggtg cgcccggagc agtgcgttca
    49981 ggtcgcgcgc ggccgcgccg gcctcggcct cgagggcatc ccggtcgcgg cgcagccggt
    50041 cgagccactc ggccgcgccg gcgtcggtct cgagcggctc ggcgtcgatc accgtggcgc
    50101 gcacgaccgg cacggggagg ggcccgtcgt cgccgcccga tcgccgcgcc cggcggcgac
    50161 cgacgagcgg gcgcggggcc gccccggacg tgcgcagcac cagcacgcgc tcgggctccg
    50221 cctccgggtc ggcgcgggtc atgtagcggc cgtcggcggg accgaggggg aacgcgaact
    50281 cgaactggac gaagcggaag cgggcgcccg ggggcggcgc ttccaagcgg ctactcgccg
    50341 cggtgggcgc tactggagcg cttgttcttc ttgtcggccg ggagcgcgta cttcgagggc
    50401 ttggtcggca cgacggggtt gtccagctcg cgcaagcggt cgatgaggct gctccactgg
    50461 ccgggcttca gcacagccag cgcctgctgc ccgagcttct tgttctcgcc gaacatcggc
    50521 tggaagccca cgtggatgtg gtcgttgtgg tcggccatcg agagcgtgtt cgacccgaag
    50581 tcgagcagcg agatgatctg ggccgggcgc atcgtgccct ggaggagcat cagccgcttc
    50641 acgccctgct cggtgacgcc gcccttgtcc tggtggccga ggatcggcac gccgttgagc
    50701 tgcgcgatgt cgaccgcgtt gccgctccag tggtgcgaca cgttgcccga gctggtcatc
    50761 ttgccgtggc cggtgcgcag cgacgtgacc gtcggccgca ggccggactc ggccaggaac
    50821 tcgagcgtgg cgagcacgcg gcggtcgatc tggcccgagc ggatgtcgtt gcgaccgccg
    50881 gggtagatgt cgacgcgctc gtcggagagg acgcgcttct ggaggaggtt cttcggcagc
    50941 aggaggatct ggccgatcga gaaggcgtcg ctggaggagt agagcgcgtt cttgccgttg
    51001 gcgcggtaga tcgccgtcga ctcgagcagc ttccagccgt cgaggatcgg cttcgggtcg
    51061 atcgtggggg cgccgcgacc ggccgggcgg atcgagaagg tgaggtgggc ggccttctgc
    51121 gcgtcgggcc ggccaacgcg gccgagcatc gtgccggcga ccacgcgagc gcccttcttc
    51181 agcggccgca ggacggcgtt ctccgcgttc agcccgtaca cgtgggcgaa caggccgcgg
    51241 taggtggcga agcccttctg ggaggtcgcg cggctgaaga tctgctcgat agccccgttc
    51301 gcgcgtggct tggaccggta ggggtgcgcg aagatgcgcg gcttgtaggt gacgcgcgcg
    51361 gtcgagtcgc tcgcctcggt cgtgacgctg tcggatgccg cgtcgggctt ctccgacgcc
    51421 tggcggccgg cggaggcggg cgcgcccgga cgcgggtcgc tcatgttggc cgcgacggcc
    51481 ttggcggagc tgtcctcgat gtcgaggtcg accttcggga cggggtagag ctgcgagacc
    51541 gagccgaggc cgctgtacgt gtactggttc ccgtagacgt cctggaggac gatgtagtgc
    51601 ccgagcttcg ggtcggtgcc gatcttcttg atgacgccgt cgttcgaggc gacgaccggc
    51661 gcgccgttgt cggagaagat gtcgatcgag cggcgatctg cgtcctcgag gacgttcgcg
    51721 gcgttctcac cgcgcttcac gcgcttcttg agctcgcgct cgaggacgtc gttcgcgtaa
    51781 cgcgcgcggg aggcgaccgg gaagcgaccc tgggtgagcc cggtgagcga gccgacgagg
    51841 tcggccggga cgcccgcgat gagcttcgcg cggagcatca ccgagtcgac gtaccagtcg
    51901 gcgtggttgt aggcgaagat cgcgcggcgg atgtccttct cagcgccggc ggccttcagg
    51961 tagttggcgg cggcgaagat cgcgtcgacc gggttgtacg gatccttctt gccgtcgtcg
    52021 ttcgcgtcga cgccgtaggc cttccaggtg agcggcatga actgcatcca cccgagggcg
    52081 ccggccgagg agacgttgag gttccggccg tagtccgtct cgatctcgtt gatcgcggcg
    52141 aggatctccc agcggacccc gtactggatg ccggcggcct ggtagatcgg caggaggaag
    52201 atcgggacct ggaacttgcg gatgacgaag ttggggactg cggtgccggg ggtcgggttc
    52261 ggcagcgcgt cgaagaacgt ggggttggtg cgggtgggcg agccgtcctg gttgcggagc
    52321 ggcgtgcgct cgcggtcgcg ctccttcgcc ttcttcttac gctccttggc ccgctcttcc
    52381 tcagccttag attgaggctc gtccgcgcgg gacggatcct tcttcttgtc gccgcggtcg
    52441 cggctctggt ccgagccgcc gtcggagctc ggcttgctgc ccttgtcgct gccggagccc
    52501 tggtcgccgc cgccggagct ggcggggggc ttggtgccct tgtcgccgcc ctggtcgccg
    52561 ccgccgtcgc cgccaccgct gggggctgcg ggctcgtcac cgccgccagg cggcgtgagc
    52621 tcctggatcg gagtgccggg caggcccggg acctggtcca tcgggacgcc cgcgggcgcg
    52681 tcgaccgtga cgcggatgac gttgccggtc ggcagcttga cgaggaacgt ccgcagctgg
    52741 gcggacgccg acatgctgaa gatcgcgaga gcactcgcaa cgacggctgc gagcaggatg
    52801 gcaaagaaga gcctcctctt catgcctggg tacctctctc ctcccaaatg ctcctcccaa
    52861 agcgggctac gacgccggcg acgcgggaag ctatcagaag ctccagcggt aggacgcacg
    52921 ctatctgacg cccatttggc ctgcaaccgg gaaccgtcgc ggtagtcatc agtgtggtga
    52981 gggaacacgc tccgcggcac ggcgcttcgg agagtgggcc agttttaccc cacctttgcg
    53041 ttgcgtatcc gcacttagtg ttgtgctccc gggaagccgg agggccgatc cggcgcgctc
    53101 gggggcgcat acgggcgcac gtctacacgc gtgcgcgcgc gaagagcctg tcggcggggg
    53161 tggtgagacc cggcgccggg gcacaagcgg aaaactgact ctaaggttca agtcgagcgt
    53221 tagattcata cggcgcgagg agtttctggc gcgaccgggc ggacgctccc ggggattcgg
    53281 tgtacgctgc gcggcccgtg gacgaagagg ctcagaccga cgagggcatc cgtgaccgcc
    53341 tcacccgcca gggcgaggag gcgctgggca agctcgccga ggagctcgtc ggcaacccgc
    53401 ttctgggcgg agccgtgagc cgcgcgttcg aggcccgcga gaaggcggtc caggcgcagg
    53461 aggccgcgat gggcgcgctg ggcatcccgt cggctgcgga cgtcgagcgc ctgacgcgcc
    53521 ggctgcggtc tgtctcgcaa cggctcgagg gcgtggagga cggtgtggat cggctggacg
    53581 cacggctcga gcggatcgag gccggggtga cggccctcga cggccagctc ggcggggcgg
    53641 atgcggactc cggccaggcg accgcggcca tcgagttgcg tctcgacgag atcgcgcgcg
    53701 acctgtccgc gctgcgcgag gcggtcgcgc cgggctccga gccgccgccg cgcgcgcagg
    53761 agcgcctcac cgtctcagac gcgcagtagc gccggcgggc tcaggccgcc agcgcgcgca
    53821 gtgcgacctc gccgaggcgg ggcgaccgct tgtcgatctc gtcgtgctcc atccgctcgc
    53881 ggccgccgcc ggcgagcagg tccgtgacca gcaggacgca gcccgcccgg gctccacgcc
    53941 gggccgcggt cgtcagcacg gcggcggcct ccatctcgat cgcggcggcg ccggcccggc
    54001 gccagccgtc cgcgcgagca gggtccgggt cgtagaagag gtccgaggag acgaccgtga
    54061 ccgcgcggcc tccgccgccg gcgagcgccg cggtgagctc tgcgtcggcc tcgacgcgct
    54121 cctgggcgcc gagcgcgcgg ctggtgccgt cggccgctat tgcctccgca accgtcacga
    54181 ggtcgcccag ccgcaggccg gcgtccaacg cgccgcaggt accggttcgc acgaaccggc
    54241 gggcgccgag cctgatcagc tcctcaacga cgatcgcggc gctcgggccg cccatgccgg
    54301 tggactggat cgtgagcgcg gcgccgtcgg acgcgcggcc ggtgtagccc cacaggccgc
    54361 ggcgggtgtt gaacatccgc ggggactcga gcagcgcctg ggccatcgtg agggcgcgct
    54421 gggggtcgcc ggggaggagc acccgttcgg ccagctccgc cgcggggttc aggtggggag
    54481 tgggctcggg cacggcccca ccgtatccgg gcgtagactg gccaccttcc atggccgagc
    54541 gaaagtcacc agcggagcgc ctgcccgacg cgcttcgaga ggcggtcgag cggacggtgc
    54601 aggccacgcg cgagtcggtc gaggccacgc gcgagtccgc gaaggggacg cgcggtcggg
    54661 cgcaggaggc ggtggacgac ctcgtgaagg gcgccgaggc cggggccgag acgctccgcg
    54721 accgggtgcg cggcgcggtg ccggccacgt ccgaggacct gaaggacctg cggcgcgagc
    54781 tgcgcgcgat cgggcgccgg ctcgacgcga tcgaggagcg cctgccggcg aa
//
LOCUS       DASVPL010000038        10090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_121343, whole genome shotgun sequence.
ACCESSION   DASVPL010000038 DASVPL010000000
VERSION     DASVPL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121343"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>10090)
                     /locus_tag="VF517_02205"
     CDS             complement(<1..>10090)
                     /locus_tag="VF517_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX8051774.1"
                     /translation="NFSEPAPEIPLDELRLRVQQTLGSWPRPDRPLLAGVSSFGMGGT
                     NCHMVLADPPVQDGGERGERGEPETPETPTGVVPWVLSGRTESAVRDQARNLLAHVEE
                     RPDLEVGDVAYSLVTSRSRFDARAVVIGRDRERLLQGLGSLSRGEPAAGVVEGHARSG
                     DTVFVFPGQGSQWEGMALELMDAEPVFAESMRACAEALSRHVDWDLEEVLRGADGAPT
                     FERVDVVQPALFAVFVSLAALWRSYGVEPSAVVGHSQGEIAAAHVAGALSLDDAARVV
                     ALRSQAVRDGLAGHGGMVSVALTPEACEERIERFGERVSLAAVNGPASVVVSGQDEAL
                     DELLAECEADDIWARRINVDYPSHSARVEALEERLARDLEPIEPRPGTVPFFSTLEAE
                     VIDTTALDAGYWYRNLRNRVRFNDAVEALIRDGATAFVECSAHPVLTAGMQEAVDATL
                     EDEAAEVVVLGSLRRDEGGPDRFMRSLAALFVHGGAIDWGRSFEGSGARRVDLPTYAF
                     QRRRYWLEELPSAAGDEPDLAASSRGGGTADDGAGAETAAADAQSGGALAQRLDGLSE
                     KDGERAVRDLVLAQVAVVLGHDSAAEVDARRPWRDLGFDSPAAVELRNGLTRVTGLRL
                     PSSLLFDYPTPVAVADYLLKEATGTRTEAAAIAPVARNDEPIAIVGMSCRYPGGVDSP
                     EALWELVESGRDAIGEFPEDRGWDLDRLFDPDPDKPGTSYTRNGGFLYDAAEFDPHAF
                     SISPREALAMDPQQRVLLEAAWEALEDAGIPPSSLKGSLTGVFVGVMPSDYGPRMHEP
                     ADETEGYGLTGVTTSVASGRLAYTFGLEGPAMSVDTACSASLVALHLACQALRSGECE
                     LALAGGATVMSNPGIFVEFSRQRGLSQDGRCKAYGAGADGTGWSEGAGLLVLEPLSYA
                     RARGHEVLALIRGSAINQDGASNGLTAPNGPSQERVIRQALASAGLEPGDVDAVEGHG
                     TGTTLGDPIEAQALLATYGKERSNGPLYLGSLKSNVGHTQAAAGVAGVIKMVEAMRHG
                     VLPRTLHVEEPSPHVDWSAGAVELLREPVEWTPRDHPRRAAVSSFGVSGTNSHLILEE
                     AAPAEEAAEEPSEVVDGPRAELRALPLLVSAHNEAALRAQARRLRAWLAERPELEPID
                     MAFSLATARAQLERRAAVVGSGREELMARLEALARGERADGVVEGQAGSGDTVFVFPG
                     QGSQWEGMALELMDAAPVFAESMRACGEAIAQYVDWDLEDVLRGADGAPTFERVDVVQ
                     PALFAVMVSLAALWRSYGVEPSAVVGHSQGEIAAACVAGALSLDDAARVVALRSQAVR
                     DDLAGRGGMVSVALTPEACEERIASFGERLSLAAVNGPASVVVSGEDAALDELLATCE
                     REDVWARRIPVDYPSHSAAVEALEERLARDLGPIEPRTGSVPFFSTVTAEVVDGATLD
                     AAYWYRGLRNRVRFNDAVEALIGQGTAAFAEMSPHPALTVGMAAAAESAESGERVAVV
                     GSLRRDEGGLDRFLTSLAEAHVGGVSVNWATLYEGTGARRVALPAYAFQRERFWLESR
                     GGAGDLAAAGQSPAEHPVLGAAVQLAGDQGWALTGRVSLATHPWLADHAVLGVMILPG
                     AGLVELALAAAEHVGAGGLEDLTLVAPLVLEGERAIQVAVAEPDDEDRRAVNIYSRPQ
                     GGVRDDTGEAEWTLHASGLLGAADAPAGEGAEELEAFARESWPPEGAEELDVEDFYDR
                     VAGAGYEYGPAFQGVRQAYQVGEVWYAEVALDEAERSHAGEFGLHPALFDAALHPMLL
                     DVMERREGDAPPEVPFSFSGVRLERGGASALRVRMEASHDADADALTLRILALDEEGE
                     PVLAIETLKARAIDQAALKSQAASGGHESLFSVEWVDVPGAAPEGASVALLGGGDEDL
                     GSALEAAAIEVARYPDLEALEEMVGAGVAAPQIVAVRTGEADGAMPEAVHATAERTLE
                     LLKAWLASEALSEARLVLLTDRALAVSQGESPNLAHAALAGLLRSAQTEYPDRFALID
                     LDGSEASADRLADALASDEPEIALRDGTLYAPRLARAKVDDESATEPPDPDGTILITG
                     GTGGLGALVARHLASEHDARHLVLTSRRGIEAEGAKELVAELAELGCEARVAACDVGE
                     REQVEAVIAEIPDEHPLTAVIHAAGVLDDGVIDSLDGERLRRTMVPKVDGALSLHELT
                     ADLELAQFVLFSSVASTLGNPGQGNYAAANAFLDGLAHHRRAQGLPATAVAWGVWERA
                     TGMTAAVSDADRTRLERFGVSELSDEEGLVLLDTARAAADPLLVAVRLDNAALRGRAK
                     AGTLPALLQGLFRVRQASSDGQGSLAKRLASAPEPEWKAIVLEVVQTHAAGVLGHSSP
                     DTIDPQRAFKELGFDSLGAVELRNRLTQATGVKLPTTLIFDHPTPAAVADLLRSQVDG
                     AERTTQVGARRTRSHGDDPIAIVGMSARFPGGVRSPEDLWELVISGSDAIGEFPEDRG
                     WDLENLFDPDPDHPGTTYARNGGFLYDAGDFDPGFFSISPREALAMDPQQRLLLEGAW
                     EALEDAGIDPASLKGSQTGVFTGVMYHDYGISGYMPPELEGFLGTGGALLSGRTAYTF
                     GFEGPAVSVDTACSSSLVAIHLACQALEQGECELALAGGTTVLATPSAFIQFSRQRAL
                     APDGRCKSFGADADGASWSEGAGLLVLERLSDARANGHEVLALVRGSAVNQDGASNGL
                     TAPNGPSQERVIHQALASAGLSPSDVDAVEGHGTGTTLGDPIEAQALLATYGKDRASG
                     PLYLGSIKSNIGHTQAAAGVAGVIKMVESLRNGLLPRSLHCEEPSPHVDWSAGDVELL
                     AAPVDWSPGERTRRAGISSFGVSGTNAHLIVEEAPALDAAPVEHGAAPRVRRGGALPF
                     AISAATGPALGAQAGRLASFLEERQDLELYDVASTLALRRAHLPERAVAVAAERSELV
                     ENLRALERGERPAGVVQGGASGDGAVAFLFTGQGSQWPGMSIDLYQEFPVFAEALDEV
                     CAELDQYLERPLREVMFAPKDLDAAELLVETRFTQTALFALEVALYRLVQSFGIAADH
                     LIGHSIGEFAAAYVAGVFSLEDGCRLVAERARLMGALPAGGAMLAVQASEDEVRESLE
                     GYEGRLAVAAVNGPTAAVVSGEEEAVAEVEALWRERDRRTTRLRVSHAFHSPLMEPML
                     DELKEVAGSVEFSEPAIPIVSNVTGEQLTADEARSPDYWVRHVRETVRFGDGVSTLRE
                     AGVTRFLELGPDRTLITLAAQAVGAEDEGQLLFASSLRGPKTPQRAAFTAFLAAAHCN
                     HVAIDWGVLLDARGAGRVGLPTYAFQHERYWLEAAGDVGDLSAAGLRANDHPLLSAGQ
                     LLAGTNEWVFSGR"
BASE COUNT         1339 a         3886 c         3603 g         1262 t
ORIGIN      
        1 gtcgcccgct gaagacccac tcgttcgtgc cggccagcag ctgcccggcg ctgagcagcg
       61 gatggtcgtt cgccctcagc cccgccgccg ataggtcgcc gacatccccg gcggcctcga
      121 gccagtagcg ctcgtgttgg aaggcgtacg tcgggaggcc cacgcgcccg gcgccgcggg
      181 cgtcgagcag gaccccccag tcgatcgcga cgtggttgca gtgcgccgcc gccaggaacg
      241 ccgtgaaggc cgcgcgctgc ggcgtcttgg gcccccgcag gctgctggcg aacagcagct
      301 ggccctcgtc ctcggcgccc acggcctgcg ccgcgagcgt gatgagcgtg cgatcggggc
      361 cgagctcgag gaagcgcgtg acccccgcct cgcgcagagt gctcacgccg tctccgaagc
      421 ggaccgtctc gcgcacgtgc cgcacccagt aatcggggga gcgagcctca tccgcggtca
      481 gctgctcgcc ggtcacgttg gagacgatcg ggatcgccgg ctccgagaac tcgacggacc
      541 ccgcgacctc cttcagctcg tcgagcatcg gctccatgag cggcgagtgg aaggcgtggc
      601 tgacgcgcag gcgcgtcgtg cgccggtcgc gctctcgcca gagcgcctcg acctccgcga
      661 cggcctcctc ctcgcccgag acgacggcgg cggtgggccc gttgaccgcc gcgaccgcga
      721 gccggccctc gtagccctcc aggctctcgc ggacctcgtc ctcggacgcc tggacggcga
      781 gcatcgcgcc tccggccggc agcgccccca tcaggcgcgc gcgctcggcc accaggcggc
      841 agccgtcctc gagcgagaac acgccggcca cgtaggcggc ggcgaactcg ccgatcgagt
      901 ggccgatcag gtggtcggcg gcgattccga agctctggac gagccggtag agcgcgactt
      961 cgagcgcgaa cagggcggtc tgggtgaagc gggtctcgac gagcagctcg gccgcgtcga
     1021 ggtccttcgg ggcgaacatc acctcgcgca gcgggcgctc gaggtattgg tcgagctcgg
     1081 cgcagacctc gtcgagcgcc tccgcgaaga cggggaactc ctggtagagg tcgatgctca
     1141 tgccgggcca ctgcgagccc tggccggtga acaggaacgc gacggcgccg tcgccgctcg
     1201 cgccgccctg cactacgccg gccggccgct cgccgcgctc gagtgcgcgg aggttctcca
     1261 cgagctccga gcgctcggct gcgaccgcca cggcccgctc cggcagatgc gcacgccgca
     1321 acgcgagcgt gctcgccacg tcgtagagct cgaggtcctg ccgctcctcg aggaacgacg
     1381 cgagccgccc cgcctgcgcg ccgagcgccg ggccggtcgc ggccgagatc gcgaacggca
     1441 gggcgccgcc cctgcggacc cgcggtgcgg ccccgtgctc gaccggcgcg gcgtcgaggg
     1501 ccggcgcctc ctccacgatc agatgcgcat tcgtgcccga gaccccgaac gaggagatcc
     1561 ccgcccgccg tgtccgctcg cccggcgacc agtcgaccgg cgcggcgagg agctcgacgt
     1621 cacccgccga ccagtccacg tgcggtgagg gctcctcgca gtgcagcgac ctcggcagca
     1681 ggccgttgcg cagggactcg accatcttga tcacgccggc gacgccggca gccgcctggg
     1741 tgtggccgat gttcgacttg atcgagccga ggtagagcgg cccggacgcc ctgtccttgc
     1801 catacgtcgc gagcagcgcc tgcgcctcga tcgggtcgcc gagcgtcgtg cccgtgccgt
     1861 gcccctcgac cgcgtcgacg tccgagggcg acaggccggc gctcgcgagc gcctggtgga
     1921 tcacgcgctc ctgcgacgga ccgttgggcg ccgtcagccc gttcgacgcg ccgtcctggt
     1981 tgaccgcgct gccacgcacg agcgccagca cctcgtgtcc gttggcccgc gcatccgaga
     2041 gccgctcgag caccaggagg cccgcgccct ccgaccagct ggcgccgtcc gcatccgccc
     2101 cgaacgactt gcagcgtccg tcgggtgcga gggcgcgctg gcgcgagaac tggatgaacg
     2161 cgctgggggt cgcgagcacc gtcgtgccac cggccagcgc gagctcgcac tcgccctgct
     2221 ccagcgcctg gcaggccagg tggatcgcga cgagcgacga cgagcaggcc gtgtcgaccg
     2281 agaccgccgg cccctcgaag ccgaacgtgt aggccgtgcg accggagagc agagccccac
     2341 cggtgccgag gaagccctcg agctccggcg gcatgtaccc gctgatgccg tagtcgtggt
     2401 acatgacgcc ggtgaagacg ccggtctggc tgcccttgag gctcgccgga tcgattccgg
     2461 cgtcctccag ggcctcccac gcgccctcga gcaggagccg ctgctggggg tccatcgcca
     2521 gcgcctcgcg cggggagatc gagaagaagc cggggtcgaa atcgccggcg tcgtagagga
     2581 agccgccgtt gcgggcgtag gtcgtcccgg ggtgatcggg atccgggtcg aagaggttct
     2641 ccaggtccca gccgcggtcc tcggggaact cgccgatcgc atcggagccg gagatgacga
     2701 gctcccagag gtcctcgggc gaccgcacgc cgcccgggaa gcgggcgctc atccccacga
     2761 tcgcgatcgg gtcgtcgccg tgcgagcgcg tgcgccgcgc ccccacctgc gtggtgcgct
     2821 ccgcgccgtc gacctgggag cgcaggaggt ccgccaccgc cgccggggtc gggtggtcga
     2881 agatcaacgt cgtcggcagc ttcacgccgg tcgcctgggt caggcggttt ctcaactcca
     2941 cggcacccag cgagtcgaag ccgagctcct tgaacgcgcg ctgtggatcg atcgtgtcgg
     3001 gcgagctgtg cccgagcacg ccggcggcat gggtctggac gacctcgagc acgatcgcct
     3061 tccactcggg ctccggcgcg ctcgcgagcc gcttcgccag cgagccctgc ccgtcggagg
     3121 acgcctgccg gacgcggaac agcccctgca ggagcgccgg cagcgttccg gccttcgccc
     3181 gcccgcgcag ggcggcgttg tcgaggcgca ccgccacgag cagggggtcg gcggcggcgc
     3241 gcgcggtgtc gaggagcacg aggccctcct cgtccgacag ctccgagacc ccgaagcgct
     3301 cgaggcgcgt gcggtcggcg tcgctcaccg cggcggtcat cccggtcgcc cgctcccaca
     3361 cgccccacgc gaccgcggtc gcgggcaggc cctgcgcgcg gcggtgatgg gccagcccgt
     3421 cgaggaatgc gttggcggcc gcgtagttgc cctgccccgg gttgcccagc gtcgaggcga
     3481 ccgacgagaa caggacgaac tgcgcgagct cgaggtcggc ggtgagctcg tgcaggctga
     3541 gcgcgccgtc gaccttcgga accatcgtcc ggcgcaggcg ctcgccgtcg agcgagtcga
     3601 tcacgccgtc gtcgagcacg ccggcggcgt ggatcacggc ggtcagcgga tgctcgtccg
     3661 ggatctccgc gatcacggcc tcgacctgct cgcgctcgcc gacgtcgcag gccgcgaccc
     3721 gtgcctcgca gccgagctcc gcgagctcgg cgacgagctc cttcgcgccc tcggcctcga
     3781 tcccgcggcg gctggtcagg acgaggtggc gcgcatcgtg ctcggaggcg aggtggcgcg
     3841 ccacgagcgc gccgaggccg cccgtgccgc cggtgatgag gatcgtgccg tccgggtccg
     3901 gaggctcggt cgcgctctcg tcgtccacct tcgcgcgtgc gagccgcggc gcgtacagcg
     3961 tgccgtccct gagggcgatc tccggctcgt cgctcgcgag cgcgtcggcc aggcggtccg
     4021 ccgacgcctc gcttccgtcg aggtcgatca gggcgaagcg gtccgggtac tccgtctggg
     4081 cgctgcggag gaggccggcg agcgcggcgt gggcgaggtt cggcgactcg ccctgcgaga
     4141 cggccagggc acgatcggtc agcagcacga gtcgcgcctc ggacagggcc tcgctcgcga
     4201 gccaggcctt cagcagctcg agcgtccgct cggcggtcgc gtgaaccgcc tcgggcatcg
     4261 cgccatcggc ctcgccggtg cgcacggcca cgatctgggg tgccgccacg ccggcaccga
     4321 ccatctcctc gagcgcctcg aggtccgggt agcgcgcgac ctcgatcgcc gcggcctcga
     4381 gtgcggagcc gaggtcctcg tcgccgccgc cgagcagcgc cacgctcgca ccctccggtg
     4441 ccgcccccgg gacatccacc cactcgaccg agaacagcga ctcgtgcccc ccgctcgccg
     4501 cctgcgactt gagcgcggcc tggtcgatgg cgcgcgcctt gagggtctcg atcgcgagca
     4561 ccggctcgcc ctcctcgtcg agcgccagga tcctgagcgt gagggcgtcc gcgtccgcgt
     4621 cgtgactcgc ctccatgcgc acccgcagcg cggaggcacc cccgcgctcg aggcgaacgc
     4681 cggagaacga gaacggcacc tccggcggcg catcgccctc gcggcgctcc atgacgtcca
     4741 gcagcatcgg gtggagcgcg gcgtcgaaca gcgccgggtg gaggccgaac tcgcccgcgt
     4801 ggctgcgctc ggcctcgtcg agcgcgacct cggcgtacca gacctcgccc acctggtacg
     4861 cctggcgcac gccctggaac gccgggccgt actcgtagcc cgcgccggcg acccggtcgt
     4921 agaagtcttc gacgtccagc tcctcggcgc cctcgggcgg ccagctctcc cgggcgaagg
     4981 cctcgagctc ctcggcgccc tcgccggccg gcgcatcggc ggcgccgagc aggcccgagg
     5041 cgtggagcgt ccattccgcc tcgccggtgt cgtcgcgcac gccgccctgc gggcgcgagt
     5101 agatgttcac cgcccggcgg tcctcgtcgt caggctccgc cacggccacc tggatcgccc
     5161 gctcgccctc gagcacgagc ggcgcgacca gcgtcaggtc ctcgagcccg cccgcgccga
     5221 cgtgctccgc ggcggccagc gccagctcca cgagccccgc tccaggcagg atcatcacgc
     5281 ccaggaccgc gtgatcggcg agccacgggt gcgtcgcgag cgacacgcgg cccgtgagcg
     5341 cccacccctg gtcgcccgcg agctggaccg ccgccccgag caccgggtgc tcggccggtg
     5401 actggccggc cgccgcgagg tcgcccgcgc cccctcggct ctcgagccag aaccgctcgc
     5461 gctggaaggc gtaggccgga agggcgaccc ggcgcgcgcc ggtgccctcg tagagggtgg
     5521 cccagttgac ggagacgccg ccgacgtgag cctcggcgag cgacgtgagg aagcggtcta
     5581 gaccgccctc gtcgcggcgc agcgagccga ccacggccac ccgctcgccg gactcggccg
     5641 actcggccgc cgcggccatg cccacggtca gcgctgggtg cgggctcatc tcggcgaacg
     5701 ccgcggtgcc ctggccgatc agcgcctcga cggcgtcgtt gaagcgcacc cggttgcgca
     5761 ggccgcggta ccagtaggcg gcgtcgaggg tggcgccgtc gaccacctcg gcggtgaccg
     5821 tggagaagaa gggcacgctc cccgtccgcg gctcgatcgg ccccaggtcg cgagcgagcc
     5881 gctcctcgag cgcctcgaca gccgccgagt gcgaggggta gtcgaccggg atccggcggg
     5941 cccagacgtc ctcccgctcg cacgtcgcga gcagctcgtc gagggccgcg tcctcgcccg
     6001 agacgacgac cgaggccggt ccgttcactg cagcgagcga gagccgctcg ccgaagctcg
     6061 cgatccgctc ctcgcaggcc tccggcgtga gcgcgacgga gaccatcccc ccgcgcccgg
     6121 ccagatcgtc gcgcacagcc tggctgcgca gggccacgac ccgagcggcg tcgtcgagcg
     6181 acagggctcc ggccacgcag gcggcggcga tctcaccctg cgagtggccc acgaccgccg
     6241 agggctccac gccgtaggag cgccacagcg ccgcgagcga gaccatcacc gcgaacagcg
     6301 ccggctggac cacgtcgacg cgctcgaagg tgggggcgcc gtcggctccg cgaaggacgt
     6361 cctcgaggtc ccagtcgacg tactgcgcga tggcctcgcc gcacgcgcgc atcgactccg
     6421 cgaacacggg cgcggcgtcc atcagctcga gcgccatccc ctcccactgc gacccctggc
     6481 cggggaagac gaagaccgtg tcgccggagc cggcctggcc ctccacgacc ccgtccgcac
     6541 gctcgccgcg tgcgagcgcc tcgagccgcg ccatgagctc ctcgcgacct gagcccacca
     6601 ccgccgcgcg gcgctcgagc tgggccctgg ccgtggccag ggagaaggcc atgtcgatcg
     6661 gctcgagctc cggccgctcg gcgagccacg cgcgcaggcg ccgcgcctgg gcgcgcaggg
     6721 cggcctcgtt gtgggcggag acgaggagcg gcagggcgcg cagctcggcg cgcggcccgt
     6781 cgaccacctc gctcggctcc tccgccgcct cctccgcggg cgcggcctcc tccaggatca
     6841 ggtgggagtt cgtgcccgag acgccgaacg aagagaccgc cgcgcgccgc gggtggtcgc
     6901 ggggcgtcca ctcgaccggc tcgcgcagga gctcgaccgc accggccgac cagtccacgt
     6961 gcggggaggg ctcctcgaca tggagcgtcc gcggcagcac accgtggcgc atcgcctcga
     7021 ccatcttgat cacgcccgcg actccggccg cggcctgggt gtggcctacg ttcgacttca
     7081 gcgacccgag gtagagcggg ccgttcgacc gctccttgcc gtaggtcgcg agcagcgcct
     7141 gcgcctcgat cgggtcgccc agcgtcgtgc ccgtgccgtg accctcgacc gcgtcgacgt
     7201 cgccgggctc gagtccggcg ctcgcgagcg cctgccggat gacccgctcc tgcgacggcc
     7261 cgttcggagc cgtcagcccg ttcgacgcgc cgtcctggtt gatcgcgctg ccgcggatca
     7321 gggcgagcac ctcgtgtcca cgcgcgcgcg cgtaactaag tggctcgagg accaggagtc
     7381 cggcgccctc ggaccacccc gtgccgtcgg cgcccgcgcc gtacgccttg cagcgcccat
     7441 cctgcgagag cccgcgctgg cgcgagaact cgacgaagat ccccgggttc gacatcacgg
     7501 tggcgccgcc cgccagggcc agctcgcact cgccgctgcg cagcgcctgg cacgccagat
     7561 gcagcgccac gagcgaggcc gagcacgccg tgtcgaccga catcgccggc ccctccaggc
     7621 cgaacgtgta ggccagccgc ccggaggcga cgctggtcgt gacgccggtg aggccgtagc
     7681 cctcggtctc gtcggccggc tcgtgcatgc gcggcccgta gtccgagggc atcaccccga
     7741 cgaacacgcc cgtgaggctt cccttgaggc tcgacggcgg gatcccggca tcctcgagcg
     7801 cctcccacgc cgcctcgagc aggacgcgct gctgcggatc catcgccagc gcctcgcgcg
     7861 gcgagatgga gaaggcgtgg ggatcgaact ccgcggcgtc gtagaggaag ccgccgttgc
     7921 gcgtgtagct cgtgcccggc ttgtccggat ccgggtcgaa gagacggtcg aggtcccagc
     7981 cgcggtcctc ggggaactcg ccgatcgcgt cacgtccgga ctcgacgagc tcccacagtg
     8041 cctcgggcga atcgacgccg ccgggatagc ggcagctcat ccccacgatc gcgatcggct
     8101 cgtcgttgcg cgcgaccggg gcgatcgccg ccgcctcggt ccgggtgccg gtcgcctcct
     8161 tgagcaggta gtcggcgacc gccacgggcg tcgggtagtc gaagagcagg ctcgacggca
     8221 ggcgcagccc cgtcaccctg gttagcccgt tgcgcagctc caccgcggcc ggtgagtcaa
     8281 agccgaggtc acgccacggc cgcctggcgt ccacctcggc cgcggagtcg tggcccagca
     8341 ccacggccac ctgggcgagc acgaggtcgc ggaccgcccg ctccccgtcc ttctcggaca
     8401 ggccgtcgag ccgctgcgcg agcgcaccgc cggattgcgc gtcggcggcc gcggtctccg
     8461 cgccggcacc gtcgtcggcc gtccccccgc cccggctcga tgcggcgagg tcgggctcgt
     8521 cgccggcggc cgacgggagc tcctcgagcc agtagcgcct gcgctggaag gcgtacgtgg
     8581 gcaggtcgac gcgccgggcc cccgaaccct cgaacgaccg cccccagtcg atcgcaccgc
     8641 cgtgtacgaa gagcgcggcg agggagcgca tgaaccggtc cgggccacct tcgtcgcggc
     8701 ggagcgagcc gagcacgacc acctcggccg cctcgtcctc cagcgtcgcg tcgacggcct
     8761 cctgcattcc cgccgtcagc acggggtgcg cgctgcactc gacgaaggcg gtggcgccgt
     8821 cccggatcag cgcctccacg gcgtcgttga agcgaacgcg gttgcgcagg ttgcggtacc
     8881 agtagccggc gtcgagcgcg gtggtgtcga tcacctcggc ttcgagggtc gagaagaacg
     8941 ggaccgtgcc cggcctgggc tcgatcggct cgaggtcgcg cgccaagcgc tcctcgagag
     9001 cctcgacgcg agcagagtgc gaggggtagt cgacgttgat ccggcgcgcc cagatgtcgt
     9061 cggcctcgca ctccgcgagc agctcgtcga gggcctcgtc ctggcccgac acgaccaccg
     9121 aggccggccc gttgaccgcg gcgagcgaca cccgctcgcc gaagcgctcg atgcgctcct
     9181 cgcatgcctc gggcgtgagc gccaccgaga ccatcccgcc gtgcccggcg agtccgtcgc
     9241 gcacggcctg gctgcgcagg gccacgacac gggcggcgtc gtcgagcgac agcgccccgg
     9301 caacgtgcgc cgcggcgatc tcaccctgcg agtggcccac gaccgccgag ggctcgaccc
     9361 cgtagctgcg ccacagcgcg gccagcgaga cgaacaccgc gaacagcgcc ggttgcacca
     9421 cgtcgacccg ctcgaacgtg ggggcgccgt ccgctccgcg caggacctcc tcgaggtccc
     9481 agtccacgtg gcgcgagagg gcctcggcgc acgctcgcat cgattcggcg aacaccggct
     9541 cggcatccat cagctcgagc gccatgccct cccactgcga gccctggccg gggaagacga
     9601 agaccgtgtc gccggagcga gcgtggccct cgacgacacc cgcggccggt tcgccgcgcg
     9661 acaggctgcc gagaccctgg agcagccgct cgcggtcgcg gccgatcacc acggcccgtg
     9721 catcgaagcg cgaacgcgac gtgacgagcg agtacgccac gtcacccacc tcgaggtccg
     9781 gccgctcctc gacgtgcgcc agcaggttgc gcgcctggtc ccgcaccgcc gactccgtcc
     9841 gccccgacag cacccacggg accaccccgg tcggcgtctc cggcgtctcc ggctcgccgc
     9901 gctcgccgcg ctcgccgccg tcctgcaccg gcgggtcggc cagaaccatg tggcagttcg
     9961 tgccgcccat gccgaacgag ctgacaccgg cgagcagcgg ccggtccgga cgcggccacg
    10021 agcccagcgt ctgctggacc cgcaggcgca gctcgtccag gggtatctcg ggggccggct
    10081 cgctgaagtt
//
LOCUS       DASVPL010000039         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_317176, whole genome shotgun sequence.
ACCESSION   DASVPL010000039 DASVPL010000000
VERSION     DASVPL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317176"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2416
                     /locus_tag="VF517_02210"
     CDS             <1..>2416
                     /locus_tag="VF517_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX8051775.1"
                     /translation="DRHLERPLLEVMFAAEGSEEAELLADTRFTQVALFALEVALARL
                     VASFGIVPDYLIGHSIGEFSAAHVAGVFSLEDGARLVAERARLMGALPEGGAMLAVEA
                     SEAEALEGLAGFEDRLALAAINAPTAAVVSGEEQAIAEVEALWRARDRRTTRLRVSHA
                     FHSQLMDPMLDDLRAVAAEVELSAPRIPIASNLTGEQLTADEATSPDYWVRHLRATVR
                     FAAGVEHLRTNGVTRFLELGPDGTLSAMAAQTAGAEDEATGDGAAASNGASAAGGDGA
                     GASEGAGSAARAGEREPLFVSALRGRKGPQREALLGFLAAAHCDGAELDWSALLDDRG
                     VGRLELPTYAFQRSRYWLEAGASAGDLTAAGQTSAEHPLLGAAVRLAGEGEGWLFTGR
                     LSLSDQPWLADHAVADVVILPGAAFVELALAVAARTGAGALEELTLVAPLVLADDAAL
                     ALQITVGEPDEEGRRAISVYSAPQAAAGAGVGAEPGAADDPAVDWTLHAQGQLGAGGA
                     DAPESVTDAELAELAAAPWPPEGADPVDVATFYERVAAAGYDYGPAFQGLRRAYRDGD
                     DWFAEVALASDQESDAPGYGVHPALSDAALHTILLDALERIDPDAAPEVPFSFAGVRL
                     QRPGAASLRVAMRTSEQEGSDARTVRILALDESGAPAFAIEALETRAVDQAALKARAS
                     TGADALFGLSWTEVGTGSGSDTTAALVGSDDSGALDGASFELVRYPDLEALAEAIAAG
                     AAAPQVVLARAAALAPPAIDAPTSDGSSASLAGSVHDLTARTLALLQSWLAAEPLAES
                     RLVLLT"
BASE COUNT          268 a          977 c          870 g          301 t
ORIGIN      
        1 tcgaccgcca cctcgagcgt cccttgctcg aggtgatgtt cgccgccgag ggctcggagg
       61 aggccgagct gctcgccgac acgcgcttca cccaggtcgc gctgttcgcg ctcgaggtcg
      121 ccctcgcgcg cctcgtcgcg agcttcggga tcgtgccgga ctacctgatc ggccactcga
      181 tcggcgagtt ctcggccgcc cacgtggccg gcgtcttctc cttggaggat ggcgctcgcc
      241 tggtcgccga gcgggcgcgc ctcatgggcg cgctgcccga gggcggcgcg atgctggccg
      301 tcgaggcgag cgaggccgag gcgctcgagg gcctcgccgg cttcgaggac cggctcgcgc
      361 tggcggccat aaatgctccg accgccgctg tcgtcagcgg tgaggagcag gcgatcgccg
      421 aggtcgaggc gctctggcgc gcccgcgacc ggcgcacgac ccgcctgcgc gtcagccacg
      481 cgttccactc gcagctgatg gacccgatgc tcgacgacct gcgcgcggtc gcggccgagg
      541 tcgagctctc cgcgccgcgg atcccgatcg cctcgaacct gaccggcgag cagctgaccg
      601 ccgacgaggc cacctcaccc gactactggg tccgccacct gcgcgcgacg gtccgcttcg
      661 ccgcgggcgt cgagcacctg cgcaccaacg gcgtgacccg cttcctggag ctcggtccgg
      721 acggcacgct cagcgcgatg gcggcgcaga ccgccggcgc ggaggacgaa gccaccggcg
      781 acggcgcggc cgcgagcaac ggcgcgagcg cggccggcgg cgacggcgcg ggcgcaagcg
      841 agggcgcggg ctccgcggca cgggcgggcg agcgcgagcc gctcttcgtc agcgccctgc
      901 gcgggcgcaa gggaccgcag cgcgaggcgc tgctcggctt cctggcggcc gcgcactgcg
      961 acggcgccga gctcgactgg agcgcgctgc tcgacgaccg cggcgtcggg cgcctcgagc
     1021 ttccgaccta cgccttccag cgctcgcgct actggctcga ggccggcgcg agcgccggcg
     1081 acctgaccgc ggccggccag acatcggccg agcacccgct gctcggcgcc gccgtgcgcc
     1141 tcgccggcga gggcgagggc tggctcttca ccggccggct gtcgctctcc gaccagccct
     1201 ggctcgccga ccatgccgtc gccgacgtcg tgatcctgcc gggcgccgcg ttcgtcgagc
     1261 tggcgctcgc ggtcgctgcg cgcaccggcg cgggtgcgct cgaggagctg acgctggtcg
     1321 cgccgctggt gctcgccgac gacgccgcgc tggcgctcca gatcaccgtc ggcgagcccg
     1381 acgaggaggg ccgccgtgcg atctccgtct actcggcgcc ccaggccgcg gccggcgccg
     1441 gtgtcggcgc ggagcccggc gccgcggacg accctgccgt ggactggacc ctgcacgcgc
     1501 agggccagct cggcgccggc ggagccgacg cccccgagag cgtcaccgac gccgagctcg
     1561 ccgagctcgc cgccgcgccc tggccgcccg agggtgccga tccagtcgac gtcgcgacct
     1621 tctacgagcg cgtcgccgcc gccggctacg actacggccc cgccttccag ggcttgcgcc
     1681 gtgcctaccg tgacggcgac gactggttcg ccgaggtcgc cctcgcctcc gaccaggagt
     1741 ccgacgcccc cggctacggc gtccacccgg cgctctcgga cgccgccctc cacacgatcc
     1801 tgctcgatgc gctcgagcgc atcgaccccg acgccgcccc cgaggtcccg ttctccttcg
     1861 ctggcgtgcg cctgcagcgc ccgggcgcag catcgctgcg ggtcgcgatg cgcacgagcg
     1921 agcaggaggg ctccgacgcg cgcacggtcc gcatcctcgc gctcgacgag tcgggcgcgc
     1981 cggcctttgc gatcgaggcg ctcgagacgc gtgcggtcga ccaggccgcg ctcaaggccc
     2041 gcgcgagcac gggtgccgac gcgctcttcg gcctcagctg gaccgaggtc ggcacaggct
     2101 ccggctccga cacgaccgcc gccctggtcg gctcggacga cagcggcgcg ctcgacggcg
     2161 cctcgttcga gctggtccgc taccccgacc tcgaggcgct cgccgaggcg atcgccgccg
     2221 gtgccgcggc tccgcaggtc gtcctcgcgc gcgcggccgc gctcgcgccg cccgccatcg
     2281 acgcgcccac cagcgacggg tcctccgcga gcctcgccgg ctcggtccac gacctcaccg
     2341 cgcgcacgct cgcgctgctc cagtcctggc tcgccgccga gccgctcgcc gagtcccgcc
     2401 tcgtgctcct gaccga
//
LOCUS       DASVPL010000040        18106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_240236, whole genome shotgun sequence.
ACCESSION   DASVPL010000040 DASVPL010000000
VERSION     DASVPL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18106
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240236"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VF517_02215"
     CDS             complement(<1..648)
                     /locus_tag="VF517_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8051776.1"
                     /translation="MVGLQSAEVGGRTVAYRELGDGPSVLFLHGWPTSSYLWRNIMPA
                     VAEHNRAIAIDLPGFGASSKPPGTTYDFALYDRTLDGFLAELGIDRVALAVHDIGGPI
                     GLHWALERPGRLTKLALLNTLVYPEFSEMVDQFIHACSTSPIREQLTSPAGLEAAMKL
                     GLADEANLTDEVLAAVVEPFGTDAERRSLADAGIGLEIEGFEEIARLLPGLEAPVR"
     gene            732..1526
                     /locus_tag="VF517_02220"
     CDS             732..1526
                     /locus_tag="VF517_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8051777.1"
                     /translation="MRRAALMLALLAATVAAPAPAAAQDAAAPAALDWRPDVDAARSY
                     AATRRGTISFAVRTGGRLYGHRTTRGVPAASVMKAMFMVAYLNHPSVRDRALHAADMD
                     LLRPMITRSDNATATRVRNFVGDWRLRRLAERVGMRRFRTAAIWGNSRVDAADQTLFF
                     ERIDQYVPERHRERALDLLASIVPSQRWGVGRVRPAGWRLYFKGGWGSGSGAVDHQVA
                     LLRRGEHRVLLAVMTTSNGTHAYGKETLRGVAKRLLRGLAEAGARR"
     gene            complement(1480..2505)
                     /locus_tag="VF517_02225"
     CDS             complement(1480..2505)
                     /locus_tag="VF517_02225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8051778.1"
                     /translation="MLLSVLDQSPIAEGSTGAEALHNTVDLARLCDELGYHRYWVAEH
                     HGGVMLAGPSPEVLIGPLAAATTRIRVGSGGVMLPHYSPLKVAESFSVLAGLFPERID
                     LALGRAAGTDPLTTFALQRDRRMAAPDDFREGLAELLGYLDDSLPADHPFARLGAVLP
                     GRPQRPVPWLLGSSMQSAVWAAELGLPYAFADFINPSGVEIAEHYRANFRPSPWLATP
                     RLIVGAWTICAETDEEAERQTAPGRMAFTLLRQGRLVELPSIEKAQRFLDGQGPPRGR
                     RLIYGAPATVRARLDELAESYGADELMLVNVMYDHAARRRSYELVADAFGLSAERPLP
                     PAPAATA"
     gene            complement(2524..4767)
                     /locus_tag="VF517_02230"
     CDS             complement(2524..4767)
                     /locus_tag="VF517_02230"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX8051779.1"
                     /translation="MRRALLLLATLVALVALPASAQAASPTLEAGVGKADITPKLGYY
                     LGGWTRADRVAGGQHTRLHARALVLKRGDRKVALVAIDLFMVPGGLVEQIGDRLAARG
                     LSEQNIVISASHTHSGPGGYANYPTLNTAAPSLETAGNPTSFSAFFDPKPADPELYRF
                     LYDQISKAIERADDDLAPAAAGWGSTQIVGLTRNRSLEAHLADHGILKEYGEGREEDD
                     PGGYEHTVDPNVDVLRVDKLVRRKKKTVRVPIGAWTTFADHGTVTKSSFQFYNQDHHG
                     SAVQVFEQRVRRAGHVPAGQEVLNVYGNGAEGDMSAGLSRDGPAASDYVGRIEASAMV
                     RAWESAGKTLQSKPKLDVRWTRICFCGQEVEGGNVDDHSELGIPFLTGSEEERGPLYD
                     VTHEHFEGRRSAVDRGAQGHKNGGPIGNVPNRVPLLTVRVGSGVIASVPGEATKEVGE
                     RIRRATMTAMTGAGVDRVAISGLANEFILYFTTPEEYERQHYEGGNTHFGKLSSVLLI
                     QELARLAGTLVRGEPAPPPVEFDPTNGVQPNGPVYGQGAASASIIDQPAASYPRLGHA
                     AIAWRGGPDGLDRPVDSAFVTAERLVKGRWTAYDSDLGLAMLWRVDGEGRHDAFWEIP
                     RYTPTGTYRFTVQGKRYKLTSREFRVEPARSLTVHQVPAPHGYSAVVLEYPEAIRDVD
                     ITTRPKYADGGTVTFRVAGRPVRVTRGKEDVAITVRVSGGQPVFVDPGSARDRHGNAN
                     GQGLALK"
     gene            complement(4781..6229)
                     /locus_tag="VF517_02235"
     CDS             complement(4781..6229)
                     /locus_tag="VF517_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8051780.1"
                     /translation="MTAVAICGAGHNGLVAAAYLARAGVEVDVFERRDVAGGACVTEE
                     LWPGVRASPGAYTLSLLRSEIVRELDLAAHGLVVTPHEPYLFAPFPDGRRVVTWSDSA
                     RTREQLARDWSEEDARGYAEWSARWEAAAARARPLMLEPADRERWLDAVGPEILDGSI
                     ADDLRGIPSEQVRVPFAIQGLIGTLAAPEDPGTAFVGFYHDLGEAAGSPGAWGYARGG
                     MGAVTAALRSAAEAAGARVHLEAPVERVIVEDGRAAGLVLEGGREVRADAVLSNADPV
                     TTSRLAGTPLEGWAQAGPVVKVMLLLDGLPDFPSWPAGEAWHGAIDIGYTLADLTAAA
                     ADARAGRPAARPWIEAACQTTADDTLAPPGKHVLSLFCQCFPGDVDAEAAADAAIARF
                     AEACPGLPDRIEGRLALGPRELERRFGISGGHIFHGEMLPGQLLEQRHPARSFGGVEG
                     LYLAGSGAHPGGAVTGAPGYLAAQAALEDLRD"
     gene            complement(6226..6849)
                     /locus_tag="VF517_02240"
     CDS             complement(6226..6849)
                     /locus_tag="VF517_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8051781.1"
                     /translation="MTSARSDARGRLPAEERREQLLDVTKALVDERGFHGVSIEAVAR
                     EAGITRPVVYGHFADLRALLEALVDREGERALRQLADVLPTDLTEGDPRATLLAGMRG
                     YLEAVQADPATWRLVLMPPEGAPAVLRERVTQGRAAVVAALAAAVGAPGAPGRESPDP
                     ELTARMMSALSDEGARLVLTDPEQYPPERILAHTRWALDLLPVDDAE"
     gene            6924..8405
                     /locus_tag="VF517_02245"
     CDS             6924..8405
                     /locus_tag="VF517_02245"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8051782.1"
                     /translation="METFDVAIVGSGFSGLGMAIRLKQQGNHDFVVLERDDDVGGTWN
                     ANTYPGCACDVPSHLYSFSFAPNPTWSQTYSRQPEIRDYLQRCADDFGIRPHVRLGTD
                     VEEASWNEDDQRWEIETSKGSFAARVLVAGMGPLTEPKIPDLPGLDDFEGEIWHSARW
                     NHDYDLHGKRVASVGTGASAIQYVPAIQPEVEQLHVFQRTAPWIMPHTNRPTTRLERR
                     LYSAFPAVQQLVRRGIYWTREALVLGFVKRPALMKVVERLAKRHIAEQVADPELRRKV
                     TPDYTVGCKRILPSNKWYPALSADNVELVTGGLAEVRANSVVGADGVEREVDAIVFGT
                     GFQVTEMPAARRLRGRDGRLLSEVWGASPKAYRGCTASGFPNFFMLLGPNTGLGHGSM
                     VYMIESQIAYVLDALRTMKQRGAAAVEVRADALEAYNADIDARMEGTVWSTGCVSWYQ
                     DDTGRNGALWPDWTWRFRQELASFDADRYEIESPRPARTPVAA"
     gene            8402..9220
                     /locus_tag="VF517_02250"
     CDS             8402..9220
                     /locus_tag="VF517_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051783.1"
                     /translation="MSKRVLITGGAGGIGAAASERLRRQGARVVGLDLHGDGADVIEC
                     DVRDQESVDRAVVEAIERLGGLDVLVNNAGVGDPQSAGEPPDATALAVLDVNLIGPWR
                     VTSAALPALRESRGRVVNVASGLAHLAVPLATAYCMSKRGLVAYSDALRLEHGDALTV
                     TTIYPGYIRTPIHNASQEAGFGLEGIVPAEPLAQAARAIARAALGRPVRDLATTRRGA
                     FSYLLARHSPRPLVDWAVLSHLRRLARGGRVSDAGLGRDLVTRLREPRRVAGGG"
     gene            9472..10152
                     /locus_tag="VF517_02255"
     CDS             9472..10152
                     /locus_tag="VF517_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX8051784.1"
                     /translation="MLVHKYLPLARGLAARYRHTHVPLEDLVQVASMGLVMAAGRFDA
                     SRGTSFASYAVPTILGELRRHLRDHGWAARVPRGLQEDVMRVTSARNELAGRLGRAPT
                     PAELAHETGMSIESVLAAVEAGGAYEAESFDQTPTSEDDPGRSLHAVIGCEEPGFDLV
                     EYGVSVRETMADLSDHEREAVRLRFIEDLTQSEIAKRIGVSQMQVSRLLSRALARLRD
                     AAGESVAA"
     gene            complement(10204..10434)
                     /locus_tag="VF517_02260"
     CDS             complement(10204..10434)
                     /locus_tag="VF517_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051785.1"
                     /translation="MDTIHIAALAGGSWIMVGFMAFLFVGVVVGYYTQAGSGITARPY
                     GKIYSGAPGAIGPSDVSGKDHREVVSWSRGTR"
     gene            10558..12255
                     /locus_tag="VF517_02265"
     CDS             10558..12255
                     /locus_tag="VF517_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX8051786.1"
                     /translation="MESTLARRPADPATTDLRTAEFVAVDVETNGLAGDDCELTEVGA
                     VLVGGGELHDEFESLVRVRRPLSRGIERFTGITQAMVETAPEPRAVLERLAPMLDGRV
                     LIAHNASFDSRVLRQAFESEGLDWPDPPAICTVAMARRLAPLAGKRKLVPLAESLGIE
                     PEGAHRALVDARTCALVFCALFPKLCAGAVSVADALELVSPQRRARRKRAPKGGPVPR
                     EQRPDLSSLPDDPGVYVFRDERGRPLYVGKSVSLRSRARAHFCQPSGWTGRAEVVDYK
                     PTSSELGALVLENRLIKRWRPPGNVKLKRTGGYVYVRCRLDIPFPVLEVAPDPAPGHA
                     VNVGPIRGRTLASDLVDQLNSLFGLRHCGRGLKLREHASAYGQMGRCLSPCLGDLDPN
                     LYRRRLDEALAPFATPGAGGERLLAMLDERLREAVAAQRYESAAVLHRRRERMALLVE
                     GLGGLLQATHAQTRLVLARHPVKERWDAFWIVAGRVADWGPLPGPDELASRTAAALER
                     RPHAARRPAIPVEEVDEIRIVQSWIAANEPPQLELEDAAAVADWVASVTCGSAPAAV"
     gene            12314..12505
                     /locus_tag="VF517_02270"
     CDS             12314..12505
                     /locus_tag="VF517_02270"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX8051787.1"
                     /translation="MGIMDKITGRAKQAAGDVTGDSSLRREGKQEERKGDAKDELANA
                     QDKADAKADEVANLERKTS"
     gene            complement(12735..13361)
                     /locus_tag="VF517_02275"
     CDS             complement(12735..13361)
                     /locus_tag="VF517_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8051788.1"
                     /translation="MANTKCLVVDDHSVVQQGLQLLFGDIDDLELVGVVQTGEEAIDA
                     VAQLQPQVVLMDVRLPGIDGVSAVKRIQAASPNTQFVMFSAYGDKRLLSDALAAGARG
                     YVMKGSPPEDLVRAIRTVASGKPFVDPSLSPALLISDAGTAAQALSEREREILQLLAE
                     GLHTEEVARRIGLSVETVKSDTKRVIAKLQADSRTHAVAIALRRALID"
     gene            13485..13928
                     /locus_tag="VF517_02280"
     CDS             13485..13928
                     /locus_tag="VF517_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8051789.1"
                     /translation="MFGSIVVGTDGSETANEAVRQATDLAKAVGARVNLVSAFEPVGN
                     QRLREERQQVPEDMQWMVNAREDVEATLKTAAESIEEAGVEVETFARQGDPADAILDV
                     AEENNADLIIVGNKGMSGAKRFLLGSVPNKVSHHAPCSVMIIRTT"
     gene            complement(13933..15276)
                     /locus_tag="VF517_02285"
     CDS             complement(13933..15276)
                     /locus_tag="VF517_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8051790.1"
                     /translation="MRIAHLLPAAVAGLMLLAPAAVAAPYAGERVVVRYEKDASRAER
                     ADVQRATGTGFDDALPGGARILRIEDGESVAATARELEDEPGVEYAVPDYRISAAQAQ
                     PTFIPNDPGRGGPGNWADLQWNFAGEFGVNAPQAWAQTRATSAPGGRGAVVAVIDSGV
                     AYKNLGRFRRAPDLYKKRWVSPYDFIDEDRSPLDEDGHGTHVTGTIAQTTNNKLGVTG
                     LAYGVKIMPLRVLDENGDGDGSDLVRALRYAARNGADVINMSVEFTADQTAGEIPEVI
                     EAMRYASRRGAVLIGVSGNGGEQRVSYPGRYSHAIAVGGTTVGGCLAEYSNEGSGLDI
                     VAPGGGQDAAPDDSQWDRDHCDSTRRARLIYQQTIWGSPSKFQLVGFEGTSEAAPHVT
                     AAAALVVATMRARGLKPTPTAVQQRLQQTARDLGAPGYDLRYGHGLLDAAAAVAP"
     gene            15339..16451
                     /locus_tag="VF517_02290"
     CDS             15339..16451
                     /locus_tag="VF517_02290"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8051791.1"
                     /translation="MALVLVTGATGFVGGHVARLLAERGDQVRVTFRDPARLDRLAGL
                     EVETVRADVLDRASMRRAMRGCDLVFHTAGFVGSRPLDRLWQVNALAPRIVVEAAAAE
                     GVGRVVHTSTVAAIGTAAHGEAADESHVYRGGEFGLAYGDSKHEGELEALATGARHGV
                     EVVVVNPSYVLGVPVDPTQAGETSTRVIGNFLAGRLPAVVDAATNIVDVEDVAAGHLL
                     AAESGVPGERYILGGYNLTWVELIDRLAVESGVERPLVVLPREVGALARVQAELGGRW
                     PVAPEAFALMAQNWRYSSRKAKRELRYRTRSLETTIARTVEWYEELHSRGVLERRGAS
                     ALSLGAAGMRLADRAGLVAAARAAERYVGRRLVTGA"
     gene            16448..16915
                     /gene="tadA"
                     /locus_tag="VF517_02295"
     CDS             16448..16915
                     /gene="tadA"
                     /locus_tag="VF517_02295"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX8051792.1"
                     /translation="MTRAPFFPRDEYFMRLALREAERAAEHDDVPIGCVIAHEGEVLA
                     AAGNERELRDDPTAHAEVLALREAGRALGTWRLSGTAMYVTLEPCAMCAGAIVLGRVP
                     RVVYGTPDPKAGAAGSVFDVLAEPRLNHRPQVAGGLLAEESADLLRSFFALRR"
     gene            17000..17088
                     /locus_tag="VF517_02300"
     tRNA            17000..17088
                     /locus_tag="VF517_02300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17034..17036,aa:Ser,seq:cga)
     gene            complement(17176..17550)
                     /locus_tag="VF517_02305"
     CDS             complement(17176..17550)
                     /locus_tag="VF517_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051793.1"
                     /translation="MIETATRRNREHVDAGRALFEAIALEDAHLGDRRFDKVFAFNVA
                     PFWLQPEAALATLREHLARDGAVYLFWDARHSAPERARDLGNELADRLRKGGFSVDGV
                     LVEDLRPVPAVCAMGCRGADER"
     gene            complement(17771..>18106)
                     /locus_tag="VF517_02310"
     CDS             complement(17771..>18106)
                     /locus_tag="VF517_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051794.1"
                     /translation="QLHIARALRDAGVDVLDDTLPNPTALVSRAAESSPDAVVVGDGP
                     SSTPDLGARLRAAAPGATLVLWRTDASVVAVLVPGAASPRVMPAPTAAQLSSELFGPS
                     DKGETRPST"
BASE COUNT         2432 a         6466 c         6718 g         2490 t
ORIGIN      
        1 tcgcacgggc gcctcgaggc ccggcagcag gcgcgcgatc tcctcgaacc cctcgatctc
       61 gaggccgatg ccggcgtccg ccagcgagcg gcgctcggcg tcggtgccga acggctcgac
      121 caccgccgcg agcacctcgt cggtcaggtt ggcctcgtcg gcgagcccca gcttcatcgc
      181 cgcctcgagc cccgcgggag acgtgagctg ctcgcggatc ggcgaggtgc tgcacgcgtg
      241 gatgaactgg tcgaccatct cgctgaactc cgggtagacg agcgtgttca gcagcgcgag
      301 cttcgtcagg cgcccgggcc gttcgagcgc ccagtgcagc ccgatcggac cgccgatgtc
      361 gtgcacggcc agagccacgc ggtcgatccc gagctctgcc aggaagccgt cgagcgtgcg
      421 gtcgtagagg gcgaagtcgt aggtggtgcc gggcggcttg ctcgacgcgc cgaagcccgg
      481 caggtcgatc gcgattgcgc ggttgtgctc ggcgaccgcg ggcatgatgt tgcgccacag
      541 gtacgacgag gtgggccagc cgtggaggaa gagcaccgac ggcccgtcgc cgagctcgcg
      601 gtaggccacg gtgcggccgc cgacctccgc gctctgtaaa ccgaccatcg gtatgcaacc
      661 taccggggga ccggtcgccg gatgcgtgac accccgcacg cgcgcgaccg ggtcccgggt
      721 acgctcgccc ggtgcgacgc gccgcgctca tgctcgccct cctggccgcg acggtcgccg
      781 ccccggcgcc cgcggccgca caggacgcgg cggcgcccgc ggcgctcgac tggcggcccg
      841 acgtggacgc cgcgcgctcc tacgccgcca cgcggcgggg gaccatctcg ttcgccgtcc
      901 gcaccggcgg gcggctctac ggccaccgca ccacgcgcgg ggtccccgcg gcgagcgtga
      961 tgaaggcgat gttcatggtc gcctacctca accacccgtc cgttcgcgac cgcgcgctgc
     1021 acgcggcgga catggacctg ctgcggccga tgatcacgcg ctcggacaac gcgaccgcca
     1081 cccgcgtacg caacttcgtc ggcgactggc gcctgcgccg gctggccgag cgcgtcggga
     1141 tgaggcgctt ccgcacggcc gcgatctggg gcaacagccg ggtggatgcg gccgaccaga
     1201 ctctcttctt cgagcgcatc gaccagtacg tgcccgagcg ccaccgcgag cgcgcgctcg
     1261 acctgctcgc gtcgatcgtc ccgtcgcagc gctggggcgt cggccgcgtg cggccggccg
     1321 gctggcggct ctacttcaag ggcggctggg gctccggcag cggcgcggtg gaccaccagg
     1381 tcgcgctgct gcgccgcggc gagcaccgcg tcctgctcgc ggtgatgacg acgtccaacg
     1441 gcacccacgc ctacggcaag gagacgctgc gcggagtggc taagcggttg ctgcgggggc
     1501 tggcggaagc gggcgctcgg cgctgaggcc gaacgcgtcg gcgaccagct cgtacgagcg
     1561 ccgccgcgcg gcgtggtcgt acatcacgtt cacgagcatc agctcgtcgg cgccgtagga
     1621 ctcggccagc tcgtcgaggc gcgcgcgcac cgtcgccggc gcgccgtaga tcagccgccg
     1681 cccgcgcggc gggccctggc cgtcgaggaa gcgctgcgcc ttttcgatcg atggcagctc
     1741 gacgagccga ccctgacgca gcagcgtgaa cgccatccgg ccgggcgccg tctgccgctc
     1801 ggcctcctcg tcggtctcgg cgcagatcgt ccaggcgccg acgatcagcc gcggcgtcgc
     1861 cagccacggc gacggcctga agttcgcgcg gtagtgctcg gcgatctcga ccccgctcgg
     1921 gttgatgaag tccgcgaacg cgtacggcag gccgagctcg gccgcccaca cggcgctctg
     1981 catcgacgag ccgagcagcc acggcaccgg ccgctgcggc cggcccggga gcaccgcgcc
     2041 gagacgcgcg aacgggtggt cggccggaag cgagtcgtcc aggtagccga gcagctcggc
     2101 gagcccttcg cggaagtcgt ccggcgcggc catccgccgg tcgcgctgga gcgcgaaggt
     2161 cgtgagcggg tcggtgccgg cggcgcggcc gagcgcgagg tcgatccgct ccgggaagag
     2221 gccggcgagc acgctgaagc tctccgccac cttgagcggg ctgtagtggg ggagcatcac
     2281 gccgccgctg ccgacgcgga tgcgggtggt agccgccgcc agcgggccga tcagcacctc
     2341 ggggctcggc cccgccagca tcacaccgcc gtgatgctcg gccacccagt agcggtggta
     2401 gcccagctcg tcgcagagcc gggcgaggtc gaccgtgttg tgcagcgcct cggcgccggt
     2461 cgatccctcg gcgatcggcg attggtcgag gacgctcagc agcatccgcc cgactgtagg
     2521 cggttacttg agcgccagtc cctggccgtt cgcgttgccg tggcggtcgc gcgcgctgcc
     2581 cgggtccacg aacaccggct gtccgcccga cacgcgcacc gtgatcgcga cgtcctcctt
     2641 gccgcgcgtg acgcgcaccg gccggccggc gacgcggaac gtaacggtgc cgccgtccgc
     2701 gtacttcggg cgcgtggtga tgtccacgtc gcggatcgcc tccgggtact cgagcacgac
     2761 ggcggagtag ccgtgcggcg cgggcacctg gtggaccgtg agcgagcgcg ccggctcgac
     2821 gcggaactcg cgcgaggtga gcttgtagcg cttgccctgg accgtgaagc ggtaggtgcc
     2881 ggtcggcgtg tagcggggga tctcccagaa cgcgtcgtgg cggccctcgc cgtccacgcg
     2941 ccagagcatc gccagaccga ggtcgctgtc gtacgcggtc cagcggccct tgaccagccg
     3001 ctcggcggtc acgaaggcgc tgtccaccgg ccggtcgagc ccgtcggggc cgccgcgcca
     3061 cgcgatcgcg gcgtggccga ggcgcgggta cgacgcggcg ggctggtcga tgatcgaggc
     3121 gctcgcggcg ccctggccgt acaccggccc gttcggctgc acgccgttgg tcgggtcgaa
     3181 ctcgaccggg ggcggcgccg gctcgccgcg cacgagcgtg cccgcgaggc gcgcgagctc
     3241 ctggatgagc aggaccgacg acagctttcc gaagtgcgtg ttgccgccct cgtagtgctg
     3301 gcgctcgtac tcctcgggcg tcgtgaagta gaggatgaac tcgttggcca ggccggagat
     3361 ggccacgcga tcgacgcccg cgccggtcat cgcggtcatg gtcgccctgc gaatccgctc
     3421 cccgacctcc ttggtggcct cgcccggcac cgaggcgatc acgccgctac cgacccgcac
     3481 ggtgagcagc gggacccggt tcggcacgtt tccgatcggt ccgccgttct tgtggccctg
     3541 cgcgccgcgg tcgaccgccg agcggcgccc ctcgaagtgc tcgtgggtga cgtcgtagag
     3601 cgggccgcgc tcctcctcgg acccggtcag gaaggggatg ccgagctcgg agtggtcgtc
     3661 gacgttgccg ccctcgacct cctggccgca gaagcagatc cgcgtccagc ggacatcgag
     3721 cttgggcttg ctctgcagcg tcttgcccgc gctctcccac gcgcgcacca tcgccgaggc
     3781 ctcgatgcgg ccgacgtagt ccgaggccgc cgggccgtca cggctgagcc cggccgacat
     3841 gtctccctcg gcgccgttgc cgtagacgtt cagcacctcc tggcccgccg gcacgtgccc
     3901 ggcgcggcgc acgcgctgct cgaagacctg aacggccgac ccgtggtggt cctggttgta
     3961 gaactggaac gaggacttgg tgacggtccc gtggtccgcg aaggtcgtcc aggccccgat
     4021 cggcacccgc acggtcttct tcttccgccg cacgagcttg tccacgcgga gcacgtcgac
     4081 gttcgggtcg accgtgtgct cgtagccgcc cgggtcgtcc tcctcgcgtc cctcgccgta
     4141 ctccttgagg attccgtgat cggccaggtg ggcctcgagg ctgcggttgc gcgtgagccc
     4201 gacgatctgc gtcgagcccc agccggcggc cgcgggcgcg aggtcgtcgt cggcccgctc
     4261 gatcgccttc gagatctggt cgtagaggaa gcggtacagc tccgggtcgg ccggcttcgg
     4321 gtcgaagaac gcggagaagc tggtcgggtt gcccgcggtc tcgaggctcg gcgccgccgt
     4381 gttcagcgtg gggtagttgg cgtagccgcc ggggccggag tgggtgtgcg aggccgagat
     4441 gacgatgttc tgctccgaga gcccgcgtgc ggcgagccgg tccccgatct gctccacgag
     4501 gccgcccggg accatgaaca ggtcgatcgc gacgagcgcg accttgcggt cgccccgctt
     4561 caggaccagc gcgcgagcgt gcagccgcgt gtgctggccg cccgcgacgc ggtcggctcg
     4621 cgtccagccg ccgaggtagt agccgagctt cggcgtgatg tccgccttgc cgacgcccgc
     4681 ctcgagcgtg ggggaggccg cctgcgcgct cgcgggcagc gcgacgaggg ccaccagggt
     4741 ggcgaggaga aggagggcac gccgcatccg cctcattgtc tcagtcgcgg aggtcttcga
     4801 gagccgcttg ggcggcgagg taccccggcg cgccagttac ggccccgccc gggtgcgccc
     4861 cggagccggc cagatacagg ccttcgacgc cgccgaagct gcgggcgggg tgacgttgct
     4921 cgagcagctg gccgggcagc atctcgccgt ggaagatgtg ccccccgctg atcccgaagc
     4981 gccgctcgag ctcgcgcggg ccgagcgcga ggcggccctc gatccggtca ggcaggcctg
     5041 ggcacgcctc ggcgaagcgg gcgatggcgg cgtcggccgc ggcctcggcg tccacgtcgc
     5101 cggggaagca ctggcagaac agcgacagca cgtgcttgcc gggaggggcg agcgtgtcgt
     5161 cggcggtggt ctggcaggcg gcctcgatcc acggccgcgc ggctgggcgg ccggcgcgcg
     5221 cgtccgcggc cgcggcggtc aggtcggcga gcgtgtagcc gatgtcgatg gccccgtgcc
     5281 acgcctcccc ggccggccac gacgggaagt cggggaggcc gtccaggagg agcatgacct
     5341 tcacgaccgg cccggcctga gcccagccct caagcggcgt gccggccagc cgggaggtcg
     5401 tgaccgggtc cgcgttcgag agcacggcgt ccgcccgcac ctcgcgcccg ccctcgagca
     5461 cgagccccgc cgcgcggccg tcctccacga tgacccgctc caccggcgcc tccaggtgaa
     5521 cgcgcgcgcc cgccgcctcg gcggccgagc gcagcgccgc ggtgaccgcg cccatccccc
     5581 cgcgcgcgta gccccacgcg ccgggactcc cggccgcctc cccgaggtcg tggtagaagc
     5641 cgacgaacgc ggtgcccggg tcctccggcg cggcgagcgt gccgatcagg ccctggatcg
     5701 cgaacggcac gcggacctgc tcggacggga tcccgcgcag gtcgtccgcg atcgagccgt
     5761 cgaggatctc cgggccgacg gcgtcgagcc agcgctcgcg gtcggccggc tcgagcatca
     5821 gcggccgggc gcgcgcggcg gcggcctccc agcgcgccga ccactcggcg tagccgcgcg
     5881 cgtcctcctc cgaccagtcg cgggcgagct gctcgcgcgt gcgcgcggag tccgaccagg
     5941 tgacaacgcg gcgaccgtcc gggaacggcg cgaacaggta cggctcgtgc ggcgtcacga
     6001 ccaggccgtg cgccgccagg tcgagctcgc gcacgatctc ggaacgcagg agcgacagcg
     6061 tgtaggcccc gggcgacgcg cgaacgccgg gccacagctc ctcggtcacg cacgcccctc
     6121 cggccacgtc gcggcgctcg aacacgtcca cctccacgcc cgcgcgcgcg aggtacgcgg
     6181 ccgcgacgag gccgttgtgc cccgcgccgc agatcgcgac ggcggtcact cggcgtcgtc
     6241 cacgggcagc aggtcgagcg cccagcgggt gtgggcgagg atccgctcgg gcgggtactg
     6301 ctcggggtcg gtgagcacca gccgcgctcc ctcgtcggag agcgcggaca tcatgcgcgc
     6361 cgtcagctct gggtcgggcg actcgcgccc cggcgcgccg ggcgcgccca ccgccgccgc
     6421 gagcgcggcg accacggccg cgcggccctg ggtcacacgc tcgcgcagga ccgcgggcgc
     6481 gccctcgggc ggcatcagca cgagccgcca ggtggcgggg tcggcctgca ccgcctcgag
     6541 gtagccgcgc atcccggcca gcagcgtcgc gcgcgggtcg ccctcggtca ggtcggtggg
     6601 gagcacgtcg gcgagctggc gcagcgcgcg ctcgccctcg cggtccacca gcgcctcgag
     6661 cagcgcccgg aggtccgcga agtgcccgta gacgaccggc cgcgtgatgc ccgcctcgcg
     6721 cgcgaccgcc tcgatcgaga cgccgtggaa cccgcgctcg tcgaccagcg ccttggtcac
     6781 gtcgagcagc tgctcgcgcc gctcctcggc cgggagccgc ccgcgtgcat cgctccgtgc
     6841 agacgtcacg gaagcccacg ttacagctcc gtaggttacg ggcatgtaag ttacgtcagc
     6901 gtaaccaagg gagggtgatc gcgatggaga cattcgacgt ggcgatcgtc ggaagcgggt
     6961 tctcgggact cggcatggcg atccgcctca agcagcaggg caaccacgac ttcgtggtgc
     7021 tcgagcgcga cgacgacgtc ggcggcacct ggaacgcgaa cacctacccg ggctgcgcct
     7081 gcgacgtccc gtcgcacctc tactcgttct cgttcgcgcc gaacccgacc tggtcccaga
     7141 cctactcgcg ccagccggag atccgcgact acctccagcg ctgcgccgac gacttcggca
     7201 tccgtcccca cgtccggctc ggcaccgacg tcgaggaggc gagctggaac gaggacgatc
     7261 agcgctggga gatcgagacc tcgaagggct cgttcgccgc ccgcgtgctg gtcgccggga
     7321 tgggcccgct gacagagccg aagatccccg acctgcccgg gctcgacgac ttcgagggcg
     7381 agatctggca ctccgcccgc tggaatcacg actacgacct gcacggcaag cgcgtcgcct
     7441 cggtcggcac cggcgcctcg gcgatccagt acgtgccggc gatccagccc gaggtcgagc
     7501 agctccacgt gttccagcgc acggcgccgt ggatcatgcc ccacacgaac cgtcccacca
     7561 cgcgcctcga gcggcgcctc tacagcgcgt tcccggcggt ccagcagctc gtgcgccgcg
     7621 gcatctactg gacgcgcgag gcgctcgtgc tcggcttcgt caagcgcccg gcgctgatga
     7681 aggtggtcga gcgcctggcc aagcggcaca tcgccgagca ggtcgccgat ccggagctgc
     7741 gccgcaaggt cacgcccgac tacacggtcg gctgcaagcg gatcctgccg tcgaacaagt
     7801 ggtacccggc gctgtcggcc gacaacgtcg agctcgtgac cggcgggctc gccgaggtcc
     7861 gcgcgaactc ggtggtgggc gccgacggcg tcgagcgcga ggtggacgcg atcgtcttcg
     7921 gcaccggctt ccaggtcacc gagatgccgg ccgcgcgcag gctgcgcggg cgcgacgggc
     7981 gcctgctgag cgaggtctgg ggcgcgagcc cgaaggcata ccgcggctgc accgcgtcgg
     8041 gcttcccgaa cttcttcatg ctgctcggcc cgaacaccgg gctcgggcac ggctcgatgg
     8101 tctacatgat cgagtcacag atcgcctacg tgttggacgc gctgcgcacg atgaagcaga
     8161 ggggcgccgc cgccgtcgag gtgcgcgccg atgcgctcga ggcctacaac gccgacatcg
     8221 acgcgcgcat ggagggcacc gtgtggagca ccggctgcgt gagctggtac caggacgaca
     8281 cgggtcgcaa cggagcgctc tggcccgact ggacctggcg cttccgacag gagctcgcga
     8341 gcttcgacgc cgaccgctac gagatcgagt cgccgcggcc ggcgcggacc ccggtggcgg
     8401 cgtgagcaag cgcgtcctaa tcaccggcgg cgcgggcggc atcggcgcgg cggcgagcga
     8461 gcggctgcgc aggcagggcg cgcgcgtggt cggcctcgac ctgcacggcg acggcgccga
     8521 cgtgatcgag tgcgacgtgc gcgaccagga gtcggtcgac cgcgcggtgg tggaggccat
     8581 cgagcggctc ggcgggctcg acgtgctcgt caacaacgcg ggcgtcggtg atccgcagag
     8641 cgccggagag ccgcccgacg cgaccgcgct ggcggtgctc gacgtgaacc tgatcggccc
     8701 gtggcgcgtg acgtccgccg cgctgcccgc cctgcgcgag tcgcgcgggc gcgtggtcaa
     8761 cgtggcgtcc ggactcgcgc acctggcggt gccgctcgcc accgcctatt gcatgagcaa
     8821 gcgtggcctc gtcgcctact cggatgcgct ccggctcgag catggcgacg cgctcaccgt
     8881 cacgacgatc tacccgggct acatccgcac cccgatccac aacgcctccc aggaggccgg
     8941 gttcgggctc gaggggatcg tgcccgccga gccgctcgcg caggccgcgc gggcgatagc
     9001 gcgcgccgcg ctcggcaggc ccgtccgcga cctcgcgacc acgcgccggg gcgccttttc
     9061 gtacctgctc gcgcggcact cgccgcggcc gctggtggac tgggccgtgc tctcgcacct
     9121 acgccgcctc gcgcgcggcg gcagggtctc ggacgcgggc ctcggacgcg atctcgtcac
     9181 gcgcctgcgc gagccgcgcc gtgtggctgg cgggggatag agaaggcgcc aatcacccgt
     9241 ttggcggagt ttgactcgac cggtaccgga tacgatgtcg gtagacgcga ttccgtccct
     9301 ctcagggggc cgggctcgca gccaaaactt tcacgatcga gacacctgag gtcgctttcg
     9361 cggccggcgc gtggatctat ctgggcctcc gggccgtaca gaaaaggatc cccccacatg
     9421 gttgtcttgg tagcccttcc cccacaagca cccgacacgg ctgagcgcga cgtgctcgtg
     9481 cacaagtacc tcccgctggc gcgcgggctg gccgcgcggt accggcacac gcacgttccg
     9541 ctcgaggacc tcgtgcaggt cgccagcatg ggcctcgtca tggcggccgg ccgcttcgac
     9601 gcgagccgcg gcacgtcgtt cgcgagctac gcggtgccga cgattctcgg cgagctgcgc
     9661 cgtcacctgc gcgaccacgg ctgggccgcg cgcgtgccgc gcgggctcca ggaggacgtg
     9721 atgcgcgtga cctcggcgcg caacgagctg gcgggccggc tgggccgtgc cccgacgccg
     9781 gccgagctgg cacacgagac cggcatgagc atcgagagcg tgctggcggc cgtcgaggcc
     9841 ggcggcgcct acgaggcgga gtcgttcgac cagacgccca cgtcggagga cgaccccggc
     9901 cgttcccttc acgccgtgat cggctgcgag gagcccggct tcgacctcgt cgagtacggc
     9961 gtctcggtgc gcgagacgat ggccgaccta tccgaccacg agcgcgaggc cgtgcggctg
    10021 cgcttcatcg aggacctcac ccagtcggag atcgccaagc gcatcggcgt ctcgcagatg
    10081 caggtctcgc ggctgctcag ccgcgcgctc gcccgcctgc gcgacgcggc cggcgagagc
    10141 gtggccgcct agccgccgtg ccctgcccgc cgccgcctca cgggcggcgg cggtgcctcg
    10201 ccgctagcgg gtaccgcggc tccaagagac cacctcgcgg tggtccttgc ccgagacgtc
    10261 gctcggcccg atggcgcccg gcgcaccgct gtagatcttg ccgtagggcc gcgcggtgat
    10321 gccgctgccc gcctgcgtgt agtagcccac gacgacgcct acgaagagga aggccatgaa
    10381 gccgaccatg atccagctgc cgcctgcgag cgctgctatg tggatggtgt ccatggcgtg
    10441 caagctatgc gtgcggcaac gcgccagagg tgaggtcagg cacatgggtg tcggcgcgca
    10501 cggaggcgat gcaacgaccc gggcatcgcg tccgccccgg gtcgtaggac tcttgggatg
    10561 gagagcaccc tcgcccgccg gccggccgat ccggccacca cggacctccg cacggcggag
    10621 ttcgtggcgg tcgacgtgga gaccaacggc ctcgccggcg acgactgcga gctgaccgag
    10681 gtcggtgccg tgctcgtcgg cggtggcgag ctgcacgacg agttcgagtc gctcgtgcgc
    10741 gtgcggcggc cgctgtcgcg cggcatcgag cgcttcaccg ggatcaccca ggcgatggtg
    10801 gagacggcgc ccgagccgcg cgcggtgctg gagcggctgg cgccgatgct cgacggccgt
    10861 gtgctgatcg cgcacaacgc ctcgttcgac agccgcgtgc tgcgccaggc gttcgagtcc
    10921 gagggtctcg actggcccga cccgcccgcg atctgcaccg tcgctatggc acgccggctg
    10981 gcgccgctcg cgggcaagcg caagctcgtg ccacttgccg agtcactcgg gatcgagccc
    11041 gagggcgcgc accgggcgct ggtcgacgcg cgcacctgcg cgctcgtgtt ctgcgccctg
    11101 ttcccgaagc tgtgcgcggg cgcggtgagc gtggccgacg cgctcgagct cgtctcgccc
    11161 cagcgccgtg cgcgtcgcaa gcgtgcgccg aagggtgggc cggtgccgcg cgagcagcgg
    11221 ccggacctca gctcgctgcc ggacgatccc ggcgtgtacg tcttccgcga cgagcgcggg
    11281 cggccgctct acgtgggcaa gtcggtgtcg ctgcgcagcc gcgcgcgggc gcacttctgc
    11341 cagccgtcgg ggtggaccgg acgcgcggag gtggtcgact acaagccgac ctcgtcggag
    11401 ctgggcgcgc tggtgctcga gaaccggctg atcaagcgct ggcgcccgcc ggggaacgtg
    11461 aagctcaagc gcaccggcgg ctacgtctac gtgcgctgcc ggctggacat cccgttcccg
    11521 gtgttggagg ttgcgccgga cccggcgccg gggcacgccg tgaacgtcgg tccgatccgc
    11581 gggcgcacgc tggcgagcga cctggtcgac caactcaact cgctgttcgg actgcgtcac
    11641 tgcggccggg ggctcaagct gcgcgagcac gcgtccgcct acggacagat gggccgctgc
    11701 ctgtcgccct gcctcggcga cctcgacccg aacctctacc gccgccgcct cgacgaggcg
    11761 ctggcgccgt tcgcgacgcc cggcgccggc ggcgagcggc tgctcgcgat gctcgacgag
    11821 cggctgcgcg aggcggtcgc cgcgcagcgc tacgagtccg ccgcggtgct gcaccgccgc
    11881 cgcgagcgga tggcgctgct cgtggagggg ctcggcggcc tgctccaggc cacgcacgcg
    11941 cagacgcggc tcgtgctcgc gcgccatccg gtcaaggagc gctgggacgc gttctggatc
    12001 gtggccgggc gcgtggcgga ctggggtccg ctgcccggcc cggacgagct ggcctcgcga
    12061 acggccgcgg cgctggagcg ccgcccgcac gccgcgcggc gcccggcgat ccccgtcgag
    12121 gaggtggacg agatccgcat cgttcagtcc tggatagcag cgaacgagcc gcctcagctg
    12181 gagctggagg acgcggccgc ggtggcggat tgggtggcat ctgtcacgtg cggatcggcg
    12241 cccgcggccg tttgaccggc gcgccccacg ggcacgcaac cagtaggcag taaccccgct
    12301 caggaggtac gacatgggaa tcatggacaa gatcacaggc cgcgcgaagc aggccgccgg
    12361 cgacgtaacc ggcgatagct cccttcgccg cgagggcaag caggaggagc gcaagggcga
    12421 cgcgaaggac gagctcgcca acgcgcagga caaggctgat gccaaggccg acgaggtcgc
    12481 caacctggag cgcaagacct cttagcccca agtaagtcgc tggggctgat ccgcaccgtc
    12541 ggaggtgcgt gaggaccctt actcgggggg ctctctgagg gagcctgcga ccgtaccccc
    12601 gaggaaggcg tccgcacgca cctccgccgc agcggcccga gctaaagccg gatgatcgcg
    12661 atcaccgcac tgatcagcac gcagatcacg atcacgatct gcatcgcgat ccagaagcgc
    12721 ggcatttcgc ccacttaatc gatgagggcg cggcggaggg cgatggccac cgcgtgcgtg
    12781 cggctgtccg cctgaagctt cgcgatcacg cgcttggtgt cggacttcac ggtctcgacc
    12841 gagagtccga tccggcgcgc gacctcctcc gtgtggagcc cctccgcgag tagctggagg
    12901 atctcccgct cgcgctcgct gagcgcctgc gccgcggtgc ccgcgtcgct gatcagcagg
    12961 gccggcgaca gcgacgggtc cacgaacggc ttgccggaag cgaccgtgcg tatcgcgcgc
    13021 acgaggtcct ccggcggcga gcccttcatc acgtatccac gcgcgcccgc ggcgagggcg
    13081 tcggagagga gccgcttgtc gccgtacgcc gagaacatca cgaactgcgt gttcggcgac
    13141 gccgcctgga tgcgcttgac cgccgagacc ccgtcgatgc cggggagccg gacgtccatc
    13201 agaacgacct ggggctggag ctgggcgacg gcgtcgatcg cttcctcgcc cgtctggacc
    13261 actccgacca gctcgaggtc gtcgatgtcg ccgaagagaa gctggaggcc ctgctggacg
    13321 acggagtggt cgtcgaccac gaggcacttg gtgttggcca tctggccaac catcctagct
    13381 ttccggccgc cagtcaagag ccgcacggcg cgcgtgggcc acgcagcggc cccctccggg
    13441 ccatatgatc cgccgcgtta aggcccggcc ttgagaggag gcagatgttc ggctcgatcg
    13501 tggtgggaac tgacggatcc gagaccgcca acgaggcggt gcggcaggcg accgaccttg
    13561 cgaaggcggt cggcgcgcgc gtgaacctgg tgagtgcgtt cgagcccgtg gggaaccagc
    13621 ggctgcgcga ggagcgtcag caggttcccg aggacatgca gtggatggtc aacgcgcgcg
    13681 aggacgtcga ggcgacgctc aagaccgcgg ccgagtcgat cgaggaggcg ggcgtcgagg
    13741 tcgagacgtt cgcgcgccag ggcgatcccg ccgacgcgat cctcgacgtg gccgaggaga
    13801 acaacgccga cctcatcatc gtcggcaaca agggcatgag cggcgccaag cggttcctgc
    13861 tcgggagcgt tccgaacaag gtctcccatc acgctccgtg cagcgtgatg atcatccgta
    13921 cgacctagcg cgctagggcg cgacggcggc cgcggcgtcc agcaggccgt ggccgtagcg
    13981 caggtcgtag ccgggcgcgc ccaggtcgcg agcggtctgc tgtaggcgct gctgcaccgc
    14041 agtgggcgtc ggcttcaggc cgcgggcgcg catcgtggcc accacgagcg cggccgccgc
    14101 ggtcacgtgc ggcgcggcct cggaggtgcc ctcgaagccg acgagctgga acttcgacgg
    14161 gctgccccag atcgtctgct ggtagatgag acgtgcgcgg cgggtgctgt cgcagtggtc
    14221 gcggtcccac tggctgtcgt cgggcgcggc gtcctggccg ccgcccggcg ccacgatgtc
    14281 gagccccgat ccctcgttcg agtactcggc caggcagccg ccgaccgtgg tgcccccgac
    14341 ggcgatcgcg tggctgtagc gaccggggta ggacacgcgc tgctcgccgc cgttgcccga
    14401 cacgccgatc aggacggcgc cccgcctact ggcgtagcgc atcgcctcga tcacctccgg
    14461 gatctcgccg gcggtctggt cggcggtgaa ctccacgctc atgttgatca cgtcggcgcc
    14521 gttgcgagcg gcgtagcgca gcgcgcgcac gaggtcgctg ccgtcgccat cgccgttctc
    14581 gtcgagcacg cgcagcggca tgatcttgac cccgtaggcg aggccggtca cgccgagctt
    14641 gttgttggtg gtctgcgcga tcgtgccggt cacgtgcgtg ccgtggccgt cctcgtcgag
    14701 cggcgagcgg tcctcgtcga tgaagtcgta cggcgagacc cagcgcttct tgtacagatc
    14761 gggcgcgcgg cggaagcgcc cgaggttctt gtaggccacg cccgagtcga tcacggcgac
    14821 gaccgccccg cggccgccgg gcgcgctcgt cgcgcgcgtc tgggcccagg cctggggggc
    14881 gttcacgccg aactcgccgg cgaagttcca ctggagatcg gcccagttgc cggggccgcc
    14941 gcggcccggg tcgttgggga tgaacgtcgg ctgggcctgc gcggcgctga tgcgatagtc
    15001 gggcaccgcg tactcgacgc cgggctcgtc ctcgagctcg cgcgcggtgg cggccacgga
    15061 ctccccgtcc tcgatccgga ggatgcgagc gccgcccggc agcgcgtcgt cgaagccggt
    15121 gccggtggcc cgctgcacgt cggcgcgctc ggcccgcgag gcgtccttct cgtagcgcac
    15181 gaccacgcgc tcgcccgcat atggcgcggc gaccgcggct ggggccagca gcatgaggcc
    15241 ggccaccgcg gccggtagga gatgggcaat tcgcattggg ctacggtgct cgagcgagtg
    15301 tacgccggag ggaacagccg aaatagcagg aagttgcagt ggcgttagtc ctcgtcacgg
    15361 gcgccacggg gttcgtcggc ggacatgttg cgcgcctgct cgcggagcgc ggcgaccagg
    15421 ttcgcgtgac cttccgggac ccggcgcgcc tcgaccggct cgcggggctc gaggtcgaga
    15481 cggtgcgggc ggacgtgctc gaccgagcgt cgatgcgcag ggcgatgcgt ggctgtgacc
    15541 tggtgttcca cacggcgggg ttcgtcgggt cgcgcccgct cgaccggctc tggcaggtga
    15601 acgccctggc tccgcggatc gtggtggagg cggccgccgc cgagggggtg gggcgcgtgg
    15661 tgcacacgtc gacagtcgcg gcgattggca cggccgctca cggcgaggcc gccgacgagt
    15721 cccacgtcta ccgcggtggc gagttcgggc tggcgtacgg cgactcgaag cacgaggggg
    15781 agctcgaggc gctggcgacc ggtgcgcggc acggggtcga ggtggtcgtg gtgaacccgt
    15841 cgtacgtgct cggggtgccg gtggacccga cgcaggcggg ggagacctcc acgcgcgtga
    15901 tcggcaactt cctcgccggc cggttgcccg cggtcgtgga cgcggcaacg aacatcgtcg
    15961 acgtcgagga cgtggcggcc gggcacctgc tcgcggccga gtccggcgtg ccgggcgagc
    16021 gctacatcct gggcggctac aacctgacgt gggtggagct gatcgaccgc ctggccgtcg
    16081 agtccggggt cgagcgcccg ctcgtggtgc tcccgcgcga ggtcggcgca ctggcgcgag
    16141 tgcaggcgga gctcggcggg cgctggccgg tcgcgccgga ggcgttcgcg ctgatggcgc
    16201 agaactggcg ctactcctcg cgcaaggcca agcgcgagct gcgctacagg acgcgttcgc
    16261 tcgagacgac gatcgcgcgg accgtggagt ggtacgagga gctgcactcc cgcggcgtgc
    16321 tcgagcggcg cggcgcctcc gcgctgtcgc tcggagcggc agggatgagg ctggcggacc
    16381 gggctgggtt ggtggccgcg gcccgcgcgg cggagcgcta cgtggggcgg cgcctcgtga
    16441 ccggcgcgtg acccgcgcgc ccttcttccc ccgcgacgag tacttcatgc ggctcgcgct
    16501 gcgcgaggcc gagcgcgccg ccgagcacga cgacgtgccg atcggctgcg tgatcgctca
    16561 cgagggcgag gtgctcgccg cggcgggcaa cgagcgcgag ctacgcgacg acccgaccgc
    16621 ccacgcggag gtgctcgccc tgcgggaggc cggccgcgcg ctcgggacct ggcggctgag
    16681 cggcacggcg atgtacgtga cgctcgagcc gtgcgcgatg tgcgcgggcg cgatcgtgct
    16741 cggccgcgtg ccgcgcgtcg tctacgggac gcccgacccg aaggccggcg ccgccggcag
    16801 cgtgttcgac gtgctcgccg agccgcggct gaaccaccgg ccgcaggtgg ccggcggcct
    16861 gctggccgag gagtcggccg acctgctgcg cagcttcttc gcgctgcggc gctagtgctc
    16921 gatcacgcgc gcgcgacgag ccacgcgcgc gtggctaacc ttccgccgtc ccggccgcga
    16981 cgtgcggcgg gcaccttacg gaggggtgcc ggagtggttg aacggggcgg tctcgaaaac
    17041 cgttaacggc ggtttcgtcg ttcgagggtt cgaatccctc cccctccgct gaagcaggcg
    17101 agtatgggct cgtttgaacc tgggctcgag agttctcacc gcatgggaac gagacgcggt
    17161 attgcgggtc cacgttcatc gttcatctgc cccacggcag cccatcgcgc agaccgcggg
    17221 gaccgggcgc aggtcctcga ccagcacccc gtcgacggag aatccgccct tgcgtagccg
    17281 gtcggccagc tcgttcccga ggtcccgggc tcgctccggc gccgagtggc gcgcgtccca
    17341 gaagaggtag acagccccgt cccgggcgag gtgctcgcgg agggttgcga gcgccgcctc
    17401 cggctgcagc cagaacggcg cgacgttgaa ggcgaagacc ttgtcgaagc gccgatcgcc
    17461 gagatgcgcg tcctcaagcg caatggcctc gaacagagcc ctgcccgcat cgacgtgctc
    17521 gcggttcctc cgcgtggccg tctcgatcat ctgggcgagc ggtcgatcgc ggtgatcgtt
    17581 cccgtcgtca gcctctcgca gaccagggaa accgcgacgc cgtggccgca gccgacctcg
    17641 aagaccgatc tactcgatga gcgtctcggt ggacggcttt atcgccggcc gtgaggggcg
    17701 cgttcgggtg gacgacccct agcgacgcgt tatctacgag cgctggacct cctcggctta
    17761 gacgccgggg tcatgtactg gggcgggtct ctcctttgtc tgaagggccg aacagctcgc
    17821 tcgagagctg ggctgcggtc ggcgcgggca tgacgcgcgg cgaggcggca ccgggaacga
    17881 gcaccgccac gacgcttgcg tccgtccgcc agagcacgag cgtcgcgccg ggtgccgcgg
    17941 cgcgcagccg cgcgccgagg tccggagtac tgctcgggcc gtcgccgacc acgaccgcat
    18001 ccgggctgct ttcggcggcc cggctcacca gcgcggtcgg gttggggagg gtgtcgtcga
    18061 ggacgtccac gcccgcgtcc ctgagcgccc gcgcgatgtg aagctg
//
LOCUS       DASVPL010000041        20762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_121957, whole genome shotgun sequence.
ACCESSION   DASVPL010000041 DASVPL010000000
VERSION     DASVPL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 20762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20762
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121957"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VF517_02315"
     CDS             <1..410
                     /locus_tag="VF517_02315"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8051795.1"
                     /translation="YCKQVLEHVEDPRPLLADVARVLRPGGWLMGSTSQLEPYHSLSV
                     GNITPYGLHRLLAAAGIDLDEVRPGIDGLTLTTHRALGMPAFTRRYWARESPLNRAVG
                     VAARARRLDARRANAAKLVLCGQFAFAGVRRPA"
     gene            581..2095
                     /locus_tag="VF517_02320"
     CDS             581..2095
                     /locus_tag="VF517_02320"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8051796.1"
                     /translation="MANGLVNELREVKPPTATAVELPARDVRAPERLLRGRLIRWETF
                     RKLLRVVILTVGDAAGVFLAIWTALELKAVIRTGANYDFGFFFDQASDYAPLACLVTI
                     LLFARSDLYADRTRRPGFARIVAAMFQVTIVILAYTIVQGERFSSYYIFYGSLLFALF
                     YVSAFRWGFDRISGMLLRAAGYRRRAVLVGTGSQIEAVAHALAGGQHGQVDPVGFVSL
                     TPRPENGLKDLGPLEELEQHFAKIDEVLIADPAFPQEQAVDLVDRCHRAGIKVCVAPS
                     TMEILMDRVEFVPGQTLPLFELKPPVFEGIDYFVKRSFDMVVASLLILFVAPVMLLSM
                     LAIKLSSRGTVIYRSMRPGIGGKPFACLKFRTMYSNADERQAELEAANEKGGALFKIR
                     NDPRVTPVGRILRRWSIDELPQLFNVLRGEMSLVGPRPLPQRDYDRLEDWHRKRNLVM
                     PGITGLWQVSGRSELDFDELVRLDFLYLERWSVFLDLTILLKTLPAVIRARGAW"
     gene            2167..2679
                     /locus_tag="VF517_02325"
     CDS             2167..2679
                     /locus_tag="VF517_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8051797.1"
                     /translation="MAEWVRSLGRAESALDLGAGDGRCAELLDARELVAADVSALALE
                     RARRRLGPDARLVELEPDAPLPLEDNAFDLVLCADTIEHVRDVQLLLSEARRVLRPGG
                     RLALSTPAHGRRTALGLLVRGFESGFDPLSPHLRFLTARSLRRLLEALGFEVESLRRD
                     GGDLLALARR"
     gene            complement(2676..4118)
                     /locus_tag="VF517_02330"
     CDS             complement(2676..4118)
                     /locus_tag="VF517_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8051798.1"
                     /translation="MRDEERYRAIVESAPDGVWMLDADDRTSYVNRAMTDMIGMEPEE
                     MLGRRPTEFVDDPTRDTVQAALDRRRAGLTERYELTFKRSDGSELITEISATPLFDED
                     GSYAGSTAIVIDATERHRARQARAELEARLQQAQRLETVGQLAGGIAHDFNNLLAVML
                     NYAHFVHEQLPEDSALRDDVEQITRAAARASELTRQLLIFSRRDPATPQAVDVNALIE
                     ETERLLKHSLGERVSLSSSPCSSRCFVHVDPAQLEHVLLNLVVNARDAMPGGGAIEIT
                     TELEDDRIVISVSDDGPGMEPEVAARAFEPFFTTKPKGAGTGLGLATVYGTIASAGGE
                     ARIETEPGSGTTIRLSLPGVSEPEEVTPDPSTGGATVLLVEDEDAVRELTRRILTKGG
                     FSCLDAPSADAALELYRESPDDIDLVLTDVVMPGMSGPELAERIEALPVLFMSGYAGD
                     AAGALPEGAPPLLEKPFSADELLAAVRAAL"
     gene            complement(4123..6300)
                     /locus_tag="VF517_02335"
     CDS             complement(4123..6300)
                     /locus_tag="VF517_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8051799.1"
                     /translation="MVRLAPAAASVALAAGFAASAFVAESGANLERTTWVEIAWIVAS
                     GLVVAVAIAFARPGRVGGWLALGGFAALTAVTAASILWSVAPDLSWIETNRALTYMWA
                     FAAALAAAHLAPRGSAVVLHALLLGAFAIVAYALVTRVFPESLAQDEVYARLGAPYGY
                     WNALGTTAGLAVPGALWLGSRRSGYGPANALAYPLLGLLFVALFLSYSRGALVVAGGA
                     TLLWLVLVPLRLRTITLLAVSLAGAAPVIGWALSRDAFTENDVPMLVRESAGPEFGIF
                     LLATVLVLLIAGLAIGFRVARRAPGATARLRVGLAAAAVAVLVPIGLVTALALSDRGI
                     PKTVTASVESLTNPNASTEGGPGRLTQASSSRGRYWRQAIDVFEENPEAGAGAGTFQV
                     TRLRHRKDQFVSRHAHGYVVQTMADLGIAGLIASVLAVLLWLGASARASGFVPRGRER
                     HHDSERVALVALALCAVAFGVHSAVDWTWAVPAPSLMAVVAGGFVAGRALRRREGATA
                     SIGWPLRAPFALATVAAALLCAWAAWQPQRSDAESQKALALIAGGKLNEADRAADRAE
                     DIDPLASKALLVRAAVEDARGNQPRAHDILEQAVERFRGEPQVWIRLAEYELYTLNRP
                     ADAEKTITGALYLDPLSRAAQQIYLEAKQAQRPTPPPAPPAAAVPGQPAPVPGAAAPA
                     QPGAAAAPQPTPGPTPGKPSGPTPRTPEPVPLTPPSGGAAPGG"
     gene            complement(6272..6886)
                     /locus_tag="VF517_02340"
     CDS             complement(6272..6886)
                     /locus_tag="VF517_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051800.1"
                     /translation="MTRFRHIALLLTAVACALAAPAPALADSDDVIRDCYEDGKLDGS
                     YTQDELKEAEDDLPSDVDEYSDCRQVIASARARGGRGKRGGTGGVPGAGFRGGGTAGA
                     DPGLSTGSGAIAGSREDLDALKSFTDSDKRPSKEPKVPVGAGQPIQPAAGATSVGESA
                     NDIPKPLLAALAAMGALGALGGLAAAWRHRPQRLRRGPLSPGRG"
     gene            complement(6904..7947)
                     /locus_tag="VF517_02345"
     CDS             complement(6904..7947)
                     /locus_tag="VF517_02345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8051801.1"
                     /translation="MKPVALVTGTVSPYRREPFRLLAEAEGVEVIAWEDPGKPVPGLT
                     VHKASQARAARLVASGRYRAVIAGLGGRVALPGTYAAARARGIPFVLWASIWSHPRTA
                     AHTLSLLPTRLLYRTADAVVTYGPHVTRHVELARRRGNVFEAPQAVSAALFGAAVPDA
                     DRRAWRERAGASETGFLALFAGRLVEEKGVPVLLDAWRAAGLEDAALALAGEGPLGSG
                     ATVLGAVPRAKLPALYAAADVLVLPSIRTATFTEPWGLVVNEAMHQGTPVITTDTVGA
                     AAGGLVRDGRNGLVVPERDAPALADALRRLAGDRALRERLGSAAKQDVAAYSEEAWVA
                     GMRRALGSVGAAR"
     gene            complement(7944..9098)
                     /locus_tag="VF517_02350"
     CDS             complement(7944..9098)
                     /locus_tag="VF517_02350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8051802.1"
                     /translation="MRVCVVAEYYPRRRDPVSGAWAHRQAVAARDAGADVRVLVLERP
                     LPSAAAVRETVRGRPGASARELRAAWAQPRHDTIDGIAVEYVRFGSPPRERSYASWHR
                     WAQRPLARALDRLHAQAPLDLVHAHYALPAGGAALPWARKSGLPLVVSVHGGDLLSPL
                     LEGSEVAKVLREADVVVANSRGMLDRAAGLAGSAERMRVIHPPGEPPPADPPPKRLEP
                     TIATLGTVDPRKRHVDVLHALAELPRDVRWVVIGAGPERARLETRAAELGLTERIEWT
                     GALPPERAIAELARCHVMALPSVDEAFGVAYAEALLCRVPAIGCAGEPGPEEIASLGP
                     GLTLVPQRDPVALAAAITEALDADPLPPAEHFSLATCGRRTVEAYEAALR"
     gene            complement(9095..10078)
                     /locus_tag="VF517_02355"
     CDS             complement(9095..10078)
                     /locus_tag="VF517_02355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8051803.1"
                     /translation="MREQGAEAGAGAAGGASAGAGAETGAEAGSAGAGAAGGAGAGAE
                     ARAPDPERFLSGSSGDPGRGLVLRARRIADRLLDAHVARAAGPRLDELAAAAPEREVL
                     VLSVYRGEGGLLPAALAELRGTRHAVRFALGSMGTAAEQLATATAATGMSGGKFENLD
                     ALLGTAGDRADWTLVVDDDVVLPTRFLDRLLGLAEHHDLALAQPAQSLASHAAWPVTR
                     RRRGSLLRVSRFVEIGPVTLFRRDALDALTPFPPLKFGWGLDLHWAAVAEERGWQLGI
                     ADALAVRHEQARVAGTYSSREAIEEAREFLAGRPFLDSAAVQETLRVHPLR"
     gene            complement(10075..10917)
                     /locus_tag="VF517_02360"
     CDS             complement(10075..10917)
                     /locus_tag="VF517_02360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX8051804.1"
                     /translation="MSPPLVSVVVPTKDRPGYLEVTLRSLRDQDLPGPYELIVVDDGT
                     DPSTRAAAHAAGARLLRPRGGRGPNTARNTGIEDAESDLVALVDDDVFAPRGWLRALV
                     EGAERHPDADAYGGPIRARLEGPAPRSCGREGPPITALDLGPEDREAELVWSANMLLR
                     RDAWERVGPFDPRFSTGGDEEEWLRRLHACGGRVVYLAAAGLDHRRAGDDARLRSLMR
                     ASYHRGRNMRSYDRMRGTAPELGGELRVLAGCGWHTVRRGCPQGLVAGAHSAGRLVEA
                     VRPR"
     gene            10941..11990
                     /locus_tag="VF517_02365"
     CDS             10941..11990
                     /locus_tag="VF517_02365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8051805.1"
                     /translation="MRQGPDIALVSLGTTPGLRRSDEAFAELVERAGATCAIVPVRIG
                     ASGRLRRHPALTDLVEALAARRSARGVEARAVVYSTITAALLQPPRVGGGAAGGGNVP
                     YGIRFDAIAALNRPGAGGLWQRRRERGVLQRARLLMPVSRGAEAAVAHVDRPRVRVPI
                     PIDPIAGAAVRDIDAVAYASYPRKRRLELLCSAWAGIDGRLVIGGADRAKGVAWLRRH
                     GVEEPAGVEWAGELPRAQWLDRVQRARVFLNASAWEDFGIAPMEALAAGTPVVTLPAA
                     GSFEALPLLRELDPRLVTNEESFADAIRTALALTDAERADYARRARELLAPYGEDAIA
                     RVVAEQVLPALGIER"
     gene            11987..13138
                     /locus_tag="VF517_02370"
     CDS             11987..13138
                     /locus_tag="VF517_02370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8051806.1"
                     /translation="MKVLVVHNRYRQRGGEERAVDLQLAALARAGIDHDVLFRDSDGA
                     GRARAARAMLRGGERPEEVGDAVRRLGADVVHVHNMHPLFGPRSLAAARDAGARVVLH
                     LHNFRLFCSIAVAFRDGETCFRCRRRFTLPGAVLDCRESAPESAVYATALARHQPLVF
                     DSVDRFVTPSHFAAGQLARLGLPAGRLDVVPNYLPAGEFAESSSAGGGTYALAFGRLS
                     REKGFEHAIAAAAASHVPLRLAGDGPLAGELRERADALGAPVEFLGAVAPEWVRELLR
                     GAAMALVPSVGPDVMPFAALEAMAAGVPVVAARSGSLPEVVGPQRCVARRDPAALAGA
                     MSALWDDSGHRADEGEAALARARELFGEERYLCELLAVYRGAGGPVLNT"
     gene            complement(13210..14178)
                     /locus_tag="VF517_02375"
     CDS             complement(13210..14178)
                     /locus_tag="VF517_02375"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051807.1"
                     /translation="MARVLVTGGAGTIGGAVVRRLVRDPEWEVRVADQREVPGWMRES
                     CEVHTGDLLDPGECAAAAEGCTHVIHLAAIVGGIANFHKLPHTLLEVNNGLFNTVFRA
                     ALHRRVERLVYVSSSMVFERATEFPTTEAHIRDTGIPDSAYGFSKLAGEVYCRALADE
                     HGLPYTICRPFNAYGPGEMPEDEPGIAHLVPDLIKKVLDGQRPLQIFGSGDQTRTLTH
                     IDDIADGVVTAMGHPAGENEDFNISASEELSVTEIARLVWEACGERPDDFLLERLPSY
                     RVDVQRRWPSVEKARELIGFEARVPLADGLRDTVAWLREQRAATPA"
     gene            complement(14206..15897)
                     /locus_tag="VF517_02380"
     CDS             complement(14206..15897)
                     /locus_tag="VF517_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8051808.1"
                     /translation="MERDGDGRGVRGGTRAGSQPLTATPRQKRLTLVACILSSAIVFV
                     DGTVVNVALPAISDDLDAGLATQQWVIESYLLMLGSLILIGGSLSDLFGRRRVFALGV
                     GGFGATSVLCAVAPSAELLIAARALQGVAGALLVPSALAVIVSTFEPSERGAAIGSWT
                     AWSGVSTLIGPFLGGVLVDAVSWRLVFAIGLLPIAATLYLIARHVPRAIDHRLDRHVD
                     VPGAVLCAVGLSGVVFALIEQPSYGWSDPLVGLPLVVGAICLALFLLQERRSPDPMLP
                     LGLFRRRNFAAGNAATFLIYGGLGGAFFFMPIFLQGVAGFSAAKAGLALVPMTIVMFL
                     LAKRFGALADRIGARPLMSLGPLVATAGILLLLRLDEDTSSLTDVVPAVLAFGLGMSM
                     VVAPLTASVLADADGEHAGIASGVNNAISRVAGLVAIAAVGALVASWFGSALDERLDA
                     ASGVSPAARAAAADAKARPLDPQPPPPGLPATQRELIESAVGDASVEAFRFAASASAL
                     LMLLGGVVSAAGMTRPRREVPCAECPGGALVGAPEDVARVAEAPRRLPEPAGAPG"
     gene            complement(15834..17069)
                     /locus_tag="VF517_02385"
     CDS             complement(15834..17069)
                     /locus_tag="VF517_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8051809.1"
                     /translation="MPADVAIVGLGRIGLPLALSFADRGLDVIGVDREPRVLDTVRAG
                     QMPFDETGVQELLERVVAGDGLRLSQRIQDASEAEFIVLTLGTPTHVHIEIDISQIRS
                     AIDALLPLLREGQTIILRSTVAPGTTEWVAGYIEQRRGFTVGQDVHVAHVPERIAVNH
                     FLDEISTLPTIVGGVGEGSGEAAARLFGVFGTEIVQTSAVEAELAKIWTNILRYTQFA
                     LPNLLMMECEQHGANVFGVIDLINHDYPRGGMAAPGLTAGTCLRKDFAFSEERSRAPG
                     MLLSVSRVHESVPNFLVEGLKKRVGGSLRDCKVAVLGLTFKRDSDDVRDSLAAKLIRL
                     LERELAHVARHDPYVTEGTETLESALSGADAVVVATNHSEYEDILPLLPHDAVIVDPW
                     NVTGTGEVFAAVRELARNR"
     gene            complement(17080..17973)
                     /locus_tag="VF517_02390"
     CDS             complement(17080..17973)
                     /locus_tag="VF517_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX8051810.1"
                     /translation="MISPEPQPITGEPLPRSLISFPETRSPSPLDYPAALPSPRDVRT
                     REILGIPIGMTDYDQALDVMDGMIARRERGYVCVAPVHSVMEAQTDAELRAALLGSSL
                     TVPDGMPVVWAANLMGENLDDRVYGPELMHRYNERCRDRGHRVWLYGGRDQGSLAQLA
                     LNMHKVNPGMRIVGGYSPPFRPLTEAEEEEIARQINAARPDVLWVGIGVPKQEKWMAH
                     MRDRLDVPVMCGVGAAFDFLAGRINQAPEWMQRRGLEWSYRIAQEPRRLLPRYVSHNP
                     RFLAAATRQILSERRRSHPRG"
     gene            complement(18104..19174)
                     /gene="rfbG"
                     /locus_tag="VF517_02395"
     CDS             complement(18104..19174)
                     /gene="rfbG"
                     /locus_tag="VF517_02395"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEX8051811.1"
                     /translation="MGVDAGFWSGRRVLVTGHTGFKGSWLSLWLQELGAEVSGFGLEA
                     PTDPALYDAARVGDGMAGVRTGDVRDRAAVADAVAALEPDVLLHLAAQPIVRASFVDP
                     VGTYETNVTGTANVLDAAVRSGVRAVVVVTSDKCYAPRADSAPCREGDPLGGADPYSS
                     SKACAELVASAWRDSFGRAGPAIATARAGNVIGGGDWAADRLVPDAVRAALAGEALMV
                     RNPDAVRPWQHVLNPLSGYLEVAQRLGEEPGAADAWNFGPAPGDELPVRAIADRMAAL
                     WGEGLEWRTDGCPPIGGHPPETAVLRLDSSRARELLGWEPRWHLDEALAATVDWFRAW
                     RGGADARAITAAQIAAFAAGGV"
     gene            complement(19165..19938)
                     /gene="rfbF"
                     /locus_tag="VF517_02400"
     CDS             complement(19165..19938)
                     /gene="rfbF"
                     /locus_tag="VF517_02400"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEX8051812.1"
                     /translation="MKAVILAGGLGTRIAEETGVKPKPMVEIGERPILWHIMKGLYAA
                     GGIDEFVVCLGYRGAVIKEWFANYYLHASDVTFDLAGNSMEVHQETAEPWKVTLIDTG
                     SATATGGRLKRVLRYVDGDEFVFTYGDGVSDLDVRALLDYHRSHGRLATVTAVQPPGR
                     WGTMAVDEDRVESFHEKPRGDGAWVNGGFFVLSPRVGDYIDGDATVWEEEPVERLARD
                     GELRAYRHEGFWRAMDTVRDRNHLEELWRTGQAPWKTWE"
     gene            complement(19935..20465)
                     /gene="rfbC"
                     /locus_tag="VF517_02405"
     CDS             complement(19935..20465)
                     /gene="rfbC"
                     /locus_tag="VF517_02405"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX8051813.1"
                     /translation="MQALATKLEGPVLIAPKVHGDARGFFQETYRRNVLADLGVGDEF
                     VQDNHSRSRLGVVRGMHFQPGQSKLVRCARGSIVDVLVDIRRGSPSFGRWEAFELNDE
                     TLHQLYVPDGFAHGFCVTSEVADVLYKCGDYYDPARESGFRYDDPEVGIDWPDVELIA
                     SERDVAAPLLSELTLP"
     gene            complement(20465..>20762)
                     /locus_tag="VF517_02410"
     CDS             complement(20465..>20762)
                     /locus_tag="VF517_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051814.1"
                     /translation="RGRPLLRRLPDLAIYTLMAAGVMLVIGHADFPAYLGGGPNFRQA
                     RYLLPLLPLYAVLLGLAARAPGRRFGPAFGAVIVLAAFAHTLFAQLLTISRFYG"
BASE COUNT         2701 a         7720 c         7676 g         2665 t
ORIGIN      
        1 tctactgcaa gcaggtgctc gagcacgtcg aggaccctcg cccgctgctc gccgacgtcg
       61 ctcgcgtgct gcgcccgggc ggctggctca tgggctcgac gtcgcagctc gagccctacc
      121 actcgctgag cgtcggcaac atcaccccgt acggcctcca ccgcctcctc gcggccgcgg
      181 ggatcgacct cgacgaggtc cggcccggca tcgacgggct cacgctcacg acccaccggg
      241 ccctgggaat gccggcgttc acgcgccgct actgggcgcg cgagtcgccg ctcaaccgcg
      301 ccgtcggcgt ggcggcccgc gctcggcgcc tcgacgctcg ccgggccaac gccgcgaagc
      361 tggtgctctg cggccagttc gcgttcgcgg gcgttcgcag accggcctga cgggtagctg
      421 gatcgctatg ctcggccctg atcgggagca ggttcgtgcg gtcgcgcgtc ctggcacagg
      481 ccagcgccac cacgtcgggg gagacggccg ttcccggtcc gataccaccg atacatttcg
      541 cccgctccgt tgttggagcc ggtgcgggtc gcactgaacg atggccaacg gactcgtcaa
      601 cgagctcaga gaggtcaagc cgccgacagc cacggcggtg gagttgcccg cgcgcgacgt
      661 ccgggccccc gagcgcctgc tgcgcggccg gctgatccgc tgggagacct tccgcaagct
      721 gctgcgcgtc gtgatcctca cggtcggcga cgccgccggc gtgttcctcg cgatctggac
      781 cgccctggag ctgaaggccg tcatccgcac tggcgcgaac tacgacttcg ggttcttctt
      841 cgaccaggcg tcggactacg ccccgctcgc gtgtctcgtc acgatcctgc tgttcgcgcg
      901 ctcggacctg tacgccgacc gcacgcgccg gcccggattc gcgcggatcg tcgcggccat
      961 gttccaggtg acgatcgtga tcctggccta cacgatcgtg cagggcgagc gcttctcgtc
     1021 ctactacatc ttctacggga gcctgctgtt cgcgctcttc tacgtctccg cgttccgctg
     1081 gggcttcgat cgcatctcgg ggatgctcct gcgcgccgcg ggctaccgcc gccgcgccgt
     1141 gctggtgggc acgggatcgc agatcgaggc cgtggcgcac gcactcgccg gcgggcagca
     1201 cgggcaggtc gaccccgtcg ggttcgtctc gctcaccccg cgccccgaga acggtctgaa
     1261 ggacctcggc ccgctcgagg agctcgagca gcacttcgcc aagatcgacg aggtcctgat
     1321 cgccgacccg gcgttcccgc aggagcaggc cgtcgacctg gtcgaccgct gccaccgcgc
     1381 cggcatcaag gtgtgcgtcg ccccgtcgac catggagatc ctgatggacc gggtcgagtt
     1441 cgtccccggc cagacgctcc cgctgttcga gctcaagccg ccggtcttcg aggggatcga
     1501 ctacttcgtc aagcgcagct tcgacatggt cgtcgcctcg ctgctgatcc tgttcgttgc
     1561 gccggtgatg ctcctgtcga tgctcgcgat caagctcagc tcgcgcggca cggtgatcta
     1621 ccgctcgatg cggccgggga tcggcggcaa gccgttcgcg tgcctgaagt tcaggacgat
     1681 gtactcgaac gccgacgagc gccaggccga gctcgaggcc gcgaacgaga agggcggcgc
     1741 cctcttcaag atccgcaacg acccgcgggt gacgccggtc ggccgcatcc tgcggcgctg
     1801 gtcgatcgac gagctgccgc agctcttcaa cgtgctccgc ggcgagatgt cgctcgtcgg
     1861 gccgcgcccg ctcccgcagc gcgactacga ccgcctcgag gactggcacc gcaagcgtaa
     1921 cctcgtgatg ccgggcatca ccggcctctg gcaggtctcg ggccgctccg agctcgactt
     1981 cgacgagctg gtacggctcg acttcctcta cctcgagcgc tggtcggtct tcctcgacct
     2041 gacgatcctg ttgaagaccc tccccgcggt catccgggcc cggggagcct ggtaggcatc
     2101 gccagccgct acgacgacga gctctgggag ctcgtccccg aggatcgcgg gccgccgccg
     2161 gcgcacctgg cggagtgggt tcgttcgctc ggccgcgccg agagcgcgct cgacctgggt
     2221 gcgggggacg ggcgttgcgc cgagctgctg gacgcgcgcg agctggtggc ggcggacgtc
     2281 tcggcgctgg cgctcgaacg ggcacgcagg cggctcgggc cggacgcccg tctggtcgag
     2341 ctcgagcccg acgcgccgct gccgctcgag gacaacgcct tcgacctcgt gctctgcgcc
     2401 gacacgatcg agcacgtgcg cgacgtccaa ctcctgctgt cggaggcgcg gcgggtgctg
     2461 cggcccggcg ggcgcctggc gctctcgacg ccggcgcacg gccgccgcac cgcgctcggc
     2521 ctgctcgtgc ggggcttcga gagcggcttc gacccgctct ccccgcacct gcgcttcctc
     2581 actgcgcgct cgctgcggcg gctactggag gcgctcgggt tcgaggtcga gtccctgcgc
     2641 cgcgacggcg gcgacctgct cgcgctcgcc cgccgctaga gcgcagcgcg cacggccgcg
     2701 agcagctcgt ccgcgctgaa cggcttctcg agcaagggcg gggccccctc gggcagcgcc
     2761 ccggcggcgt cgcccgcgta gccggacatg aacaggaccg gcagggcctc gatccgctcc
     2821 gcgagctcgg ggcccgacat ccccggcatg accacgtctg tcagcacgag gtcgatgtcg
     2881 tccgggctct cgcggtagag ctcgagggcg gcgtcggcgc tcggcgcgtc gaggcagctg
     2941 aagccaccct tcgtgaggat ccgccgcgtc agctcgcgca cggcgtcctc gtcctcgacc
     3001 agcaggacgg tggcgccgcc ggtgctggga tccggtgtga cctcctcggg ctcggacacg
     3061 ccgggcagcg acaggcggat ggtcgtgccc gaccccggct cggtctcgat ccgagcctcg
     3121 ccgcccgcgg acgcgatcgt gccatagacc gtcgccaggc cgagcccggt cccggcgccc
     3181 ttcggcttgg tcgtgaagaa cggctcgaac gcgcgcgcgg ccacctcggg ctccattccc
     3241 ggcccgtcgt ccgagaccga gatgacgatg cgatcgtcct cgagctctgt cgtgatctcg
     3301 atcgcaccgc cgcccggcat ggcgtcgcgc gcgttgacca cgaggttgag caggacgtgc
     3361 tcgagctgcg cggggtcgac gtggacgaag cagcgcgacg agcacggcga gctcgacagg
     3421 ctcacccgct ctccgagcga gtgcttgagc agccgctcgg tctcctcgat gagcgcgttc
     3481 acgtcgaccg cctgcggcgt cgccgggtcg cggcggctga agatgaggag ctggcgcgtg
     3541 agctcggagg cgcgagcggc ggcgcgcgtg atctgctcca cgtcgtcgcg cagcgcgctg
     3601 tcctcgggca gctgctcgtg gacgaagtgc gcgtagttga gcatcaccgc gaggaggttg
     3661 ttgaagtcgt gcgcgatgcc gccggcgagc tggccgacgg tctcgagccg ctgggcctgc
     3721 tggaggcgag cctcgagctc ggcgcgcgcc tgccgggcgc ggtggcgctc ggtggcatcg
     3781 atgacgatcg cggtcgaccc cgcgtacgag ccgtcctcgt cgaacagcgg ggtggcggag
     3841 atctcggtga tgagctcgct gccgtcgctg cgcttgaagg tcagctcgta gcgctcggtc
     3901 aggcctgcgc gccggcggtc gagcgccgcc tggaccgtgt cgcgcgtggg gtcgtccacg
     3961 aactcggtgg gacgccggcc gagcatctcc tcgggctcca tgccgatcat gtcggtcatc
     4021 gcgcggttca cgtagctggt ccggtcgtcg gcgtcgagca tccagacgcc gtccggggcg
     4081 gactcgacga tcgcgcggta gcgctcctcg tcgcgcatcg ggctagcccc cgggcgctgc
     4141 tccgccgctg ggcggcgtca gagggacggg ttccggcgtg cgcggagtgg ggccggaggg
     4201 cttgccgggc gtgggaccgg gcgtgggctg gggggcagcc gcggcgccgg gctgggcggg
     4261 agccgcggcg ccggggaccg gtgcgggctg gcccggcacc gcggccgccg ggggtgccgg
     4321 cggcggggtc ggccgctggg cctgcttcgc ctccaggtag atctgctgcg cggcgcgcga
     4381 gagcgggtcg aggtagagcg cgccggtgat ggtcttctcc gcgtcggccg gccggttcag
     4441 cgtgtagagc tcgtactccg ccaggcggat ccagacctgc ggctccccgc ggaagcgctc
     4501 gaccgcctgc tcgaggatgt cgtgtgcacg cggctggttc ccgcgggcgt cctccactgc
     4561 cgccctcacg agcagggctt tcgacgcgag ggggtcgatg tcctcggcac ggtcggccgc
     4621 cctgtcggcc tcgttgagct tgccgccggc gatcagggcg agcgccttct gtgactcggc
     4681 gtccgagcgc tgcggctgcc aggcggccca cgcgcacagc agggcagcgg ccaccgtggc
     4741 cagcgcgaag ggcgcgcgca gcggccagcc gatcgacgcg gtcgcgccct cgcgccggcg
     4801 cagggcgcgc ccggccacga agccgcccgc cacgaccgcc atgagcgacg gcgcgggcac
     4861 cgcccaggtc cagtcgaccg ccgagtggac tccgaaggcg accgcgcaca gcgcgagggc
     4921 gacgagcgcc acgcgctcgg agtcgtggtg gcgctcgcgg ccccggggca cgaacccgga
     4981 cgcgcgcgcg gaggcgccga gccacagcag taccgcaagc accgaggcga tcaggccggc
     5041 gatgccgagg tcggccatcg tctgcaccac gtatccgtgc gcgtggcgcg agacgaactg
     5101 gtccttgcgg tggcgcagcc gggtcacctg gaacgtgccc gcgcccgcgc cggcctcggg
     5161 gttctcctcg aacacgtcga tggcctggcg ccagtagcgc ccgcggctag acgaggcctg
     5221 cgtgagccgc ccgggcccgc cctcggtgct ggcgttgggg ttcgtgagcg actccacgct
     5281 cgcggtcacg gtcttcggga tcccgcgatc gctcagcgcg agcgcggtca cgaggccgat
     5341 cggcacgagc accgccacgg ccgcggccgc gagcccgaca cgcagccgcg cggtcgcgcc
     5401 gggggcgcgc cgggccacgc ggaagccgat cgcgagtccg gcgatcagca gcacgagcac
     5461 cgtggccagg aggaagatcc cgaactccgg cccggcgctc tcgcgcacga gcatcgggac
     5521 gtcgttctcg gtgaacgcgt cgcgcgagag cgcccacccg atcacgggcg ccgcgccggc
     5581 gagcgagacc gcgagcaggg tgatcgtgcg caggcgcagc ggcaccagga ccagccacag
     5641 cagggtcgcc ccgccggcca cgaccagcgc tccgcgcgag tagctgagga acagcgccac
     5701 gaacagcagg ccgagcagcg ggtacgcgag cgcgttggcg ggtccgtagc cggagcgccg
     5761 cgaaccgagc cagagcgccc cgggcaccgc gagcccggcg gtcgtgccga gcgcgttcca
     5821 gtacccgtac ggcgcgccga gccgcgcgta gacctcgtcc tgcgcgagcg actcggggaa
     5881 cacgcgcgtg acgagcgcgt aggcgacgat cgcgaaggcc ccgagcagca gcgcgtggag
     5941 cacgacggcg gagccgcgcg gcgcaaggtg ggcggccgcc agcgccgccg cgaacgccca
     6001 catgtacgtg agcgcgcggt tggtctcgat ccacgagagg tcgggggcga ccgaccacag
     6061 gatcgatgcg gccgtgaccg cggtcagcgc cgcgaagccg ccgagtgcca gccagccgcc
     6121 gacccggccc ggtcgcgcga acgcgatcgc gaccgccacc acgaggcccg acgcgacgat
     6181 ccaggcgatc tccacccagg tcgtccgctc gaggttcgcg ccggactccg cgacaaaggc
     6241 actcgccgcg aaccccgcgg cgagcgcgac gctagccgcg gccggggcta agcggaccac
     6301 ggcgaagtcg ctgcggccgg tggcgccacg cggcggcgag gccgccgagc gcaccgaggg
     6361 cacccatcgc ggcgagggcc gccagcagcg gcttgggaat gtcgttggcg gactcgccga
     6421 cgctggtcgc gcccgcggcc ggctggatcg gctgaccggc gccgacgggc accttcggct
     6481 ccttggaggg acgcttgtcg ctgtccgtga acgacttgag cgcgtcgagg tcctcgcgcg
     6541 agccggcgat cgcaccggag ccggtggaga gcccggggtc ggcacctgcc gtgccgccgc
     6601 cgcggaaccc tgcgccgggg acgccgccgg tgccgccgcg cttgccgcga ccgccgcggg
     6661 cgcgggccga tgcgatcacc tggcggcagt cgctgtactc gtcgacgtcg ctcgggaggt
     6721 cgtcctcggc ctccttcagc tcgtcctgcg tgtagctgcc atcgagcttg ccgtcctcgt
     6781 aacagtcgcg gatcacgtcg tcggagtcgg ccagcgcagg ggccggcgcg gcgagcgcgc
     6841 acgcgaccgc cgtgagcagc agggcaatgt gcctgaaccg cgtcacgtcg cgtatcgacg
     6901 ctatcaacgc gccgctccca cggagcccag agcgcggcgc attcccgcca cccaggcctc
     6961 ctcggaatag gccgccacgt cctgcttcgc cgcgctgccg agccgctcgc gcagggcgcg
     7021 gtcgcccgcg agccggcgca gcgcgtccgc gagtgcgggg gcgtcgcgct caggcacgac
     7081 gagcccgttg cggccgtcgc ggaccagccc accggcggcg gccccgactg tgtcggttgt
     7141 tatgaccggc gtcccctggt gcatggcctc gttcacgacc aacccccacg gctccgtgaa
     7201 agttgcggtt cggatcgagg gcagcacgag cacgtcggcg gccgcgtaga gggcgggcag
     7261 cttcgcgcgc gggaccgctc cgagcacggt cgcgccggag ccgaggggac cctcgccggc
     7321 cagcgcgagc gcggcgtcct cgagccccgc ggcgcgccag gcgtcgagca gcacgggcac
     7381 tcccttctcc tccaccaggc gtccggcgaa cagcgcgagg aaacctgtct cggaggcgcc
     7441 cgcgcgttcc cgccaggccc gccggtcggc gtccggcact gcggccccga acagcgcggc
     7501 ggagaccgcc tgcggcgcct cgaagacgtt gccgcgccgg cgcgcgagct cgacgtggcg
     7561 ggtcacgtgc gggccgtagg tcacgacggc gtcggcggtt cggtagagca gacgggtggg
     7621 gaggagcgag agcgtgtgcg cggccgtgcg ggggtgcgac cagatcgagg cccagagcac
     7681 gaacgggatg cccctcgcgc gcgcggccgc gtacgtgccg ggcagcgcca cgcggccgcc
     7741 gagaccggcg atcaccgcgc ggtagcggcc ggaggcgacg aggcgagcgg cccgcgcctg
     7801 gctcgctttg tggacggtca ggccggggac cggcttgccc gggtcctccc acgcgatcac
     7861 ctcgacgccc tccgcctcgg cgagcaggcg gaacggctcg cgccggtacg gcgacacggt
     7921 gccggtgacg agcgcgacgg gcttcaccgc agtgctgcct cgtacgcctc aacggtgcgc
     7981 cggccgcagg tcgccagcga gaagtgctcg gccgggggga gtgggtccgc gtccagcgcc
     8041 tccgtgatcg cggcggcgag cgcgaccggg tcgcgctgcg ggaccagcgt gaggcccggc
     8101 ccgagcgacg cgatctcctc cggcccgggc tcgcccgcac agccgatcgc cggcacccgg
     8161 cagaggagcg cctcggcgta ggcgacgccg aacgcctcgt cgacgctcgg cagcgccatc
     8221 acgtggcagc gggccagctc ggcgatcgcg cgctctgggg gcagcgcacc cgtccactcg
     8281 atgcgctcgg tcagcccgag ctccgccgct cgggtctcga ggcgcgcgcg ctcgggaccg
     8341 gcgccgatca cgacccagcg cacgtcccgc ggaagctccg cgagcgcgtg aagcacgtcg
     8401 acgtggcgct tgcgggggtc cacggtgccg agcgtggcga tcgtgggctc gaggcgtttc
     8461 gggggcgggt cggccggcgg cggctcgccg ggcgggtgga tcacgcgcat ccgctcggcc
     8521 gagccggcca ggccggcggc gcggtccagc atcccgcggc tgttggcgac gaccacgtcc
     8581 gcctcccgga ggaccttcgc cacctcgctt ccctccagca gcggggagag gaggtcgccg
     8641 ccgtggaccg acacgacgag cggcagcccg ctcttgcgag cccaggggag cgccgccccg
     8701 ccggcgggga gcgcgtagtg cgcgtggacg aggtcgagcg gcgcctgggc gtgcaggcgg
     8761 tcgagcgcgc gcgccagcgg ccgctgcgcc cagcggtgcc agctcgcgta ggaacgctcg
     8821 cgcgggggcg agccgaagcg gacgtactcg accgcgatgc cgtcgatcgt gtcgtggcgc
     8881 ggctgggccc acgcggcgcg cagctcgcgg gcgctcgcgc cggggcgccc gcgcaccgtc
     8941 tcgcgcacgg cggcggcgct cggcagcggt cgttcgagca cgagcacacg cacgtcggcg
     9001 cccgcgtcgc gtgccgccac ggcctggcgg tgagcccacg cgccggacac cgggtcgcgc
     9061 cggcgcgggt agtactcggc gacgacgcag accctcatcg cagcggatgc acgcggagcg
     9121 tctcctgcac ggcggcgctg tcgaggaacg gccggccggc taggaattcc cgggcctcct
     9181 cgatcgcctc ccgcgacgag tacgtgcccg ccacccgcgc ctgctcgtgg cggacggcga
     9241 gggcatcggc gatcccgagt tgccatccgc gctcctcggc cacggcggcc cagtggaggt
     9301 cgaggcccca gccgaatttc agcggcggga agggggtgag cgcgtcgagg gcgtcgcggc
     9361 gaaagagcgt gacggggccg atctccacaa agcggctcac gcgcagcagc gacccgcgcc
     9421 ggcggcgcgt gacgggccag gcggcgtggc tcgcgagcga ctgggccggc tgggccagcg
     9481 cgaggtcgtg gtgctcggcg aggccgagga ggcggtcgag gaagcgggtg gggaggacca
     9541 cgtcgtcgtc gaccacgagc gtccagtcgg cgcggtcgcc ggcggtgccg agcagtgcgt
     9601 cgaggttctc gaacttcccg ccgctcatgc cggtggcggc ggtcgcggtt gcgagctgct
     9661 cggcagccgt gcccatcgac ccgagggcga agcggacggc gtggcgcgtg ccgcgcagct
     9721 ccgcgagcgc cgcgggcagc agcccgccct ctccgcggta gaccgagagc acgagcacct
     9781 cgcgttcggg tgcggcggcg gcgagctcgt cgaggcgcgg gccggccgcg cgcgcgacgt
     9841 gcgcgtcgag cagccggtcg gcgatccgcc gcgcccgcag cacgagcccg cgaccggggt
     9901 cgccgctgga gccggagagg aagcgctcgg ggtcgggcgc gcgggcctcc gcgcccgcgc
     9961 cggcccctcc ggccgcgccc gcgccggccg agcccgcctc cgcgccggtc tccgcgcccg
    10021 cgcccgcgct ggcccctccg gccgcgcccg cacccgcctc cgcgccctgc tccctcaccg
    10081 cgggcgcacc gcctccacca gccgccccgc cgagtgcgcg cccgccacca gtccctgcgg
    10141 gcagccgcgg cgcacggtgt gccagccgca gccggccagc acgcgcagct cgccgcccag
    10201 ctcgggcgcc gtgccgcgca tgcggtcgta cgagcgcatg ttgcgcccgc ggtggtagga
    10261 cgcgcgcatc agcgagcgca ggcgcgcgtc gtcgcccgcg cgccggtggt cgaggcccgc
    10321 cgccgccagg tacaccaccc gcccgccgca ggcgtgcagg cggcgcagcc actcctcctc
    10381 gtcgccgccc gtcgagaagc gcgggtcgaa cggcccgacc cgctcccacg catcccggcg
    10441 cagcagcatg ttcgcgctcc acacgagctc cgcctcgcga tcctcgggac cgaggtccag
    10501 cgccgtgatc ggcggcccct cgcgcccgca gctgcgcggc gccgggccct cgaggcgcgc
    10561 tcgtatcggc ccgccgtacg catcggcgtc cgggtgccgc tcggcgccct ccaccagcgc
    10621 gcgcagccag ccgcgcggag cgaacacgtc gtcgtccacc agcgcgacca ggtcggactc
    10681 ggcgtcctcg atccccgtgt tgcgggccgt gttcggtccg cgcccgccgc gcggtcgcag
    10741 cagccgcgct cccgcggcgt gggcggccgc ccgcgtggac ggatcggtgc cgtcgtcgac
    10801 gacgatcagc tcgtacgggc cgggcaggtc ctggtcgcgc agcgaccgga gcgtgacctc
    10861 caggtagccg gggcggtcct tggtcggcac gaccaccgac acgaggggtg gagacacgct
    10921 gcgaggttac cctcaggccc ttgcgccagg gccccgacat cgcgctcgtc tcgctcggga
    10981 cgacgcccgg gctgcgccgc tccgacgagg ccttcgcgga gctcgtcgag cgcgccggcg
    11041 cgacctgcgc gatcgtcccg gtgcggatcg gcgcgtcggg caggctgcgc cgccacccgg
    11101 cgctgaccga cctggtcgag gcgctagcgg cgcggcgctc ggcccgcggg gtcgaggcac
    11161 gcgcggtggt gtattcgacg atcaccgccg cgctgctcca gccgccccgg gtcggcgggg
    11221 gagccgccgg cggcggcaac gtgccctacg gcatccgctt cgacgcgatc gcggcgctca
    11281 accgccccgg cgccggcggc ctctggcagc gccgccgcga gcgcggtgtg cttcagcgcg
    11341 cgcggctgct catgccggtc agccgcgggg cggaggcggc cgtcgcgcac gtcgaccgcc
    11401 cgcgtgtgcg cgtgccgatc ccgatcgacc cgatcgcggg cgccgcggtg cgcgacatcg
    11461 acgccgtcgc gtacgcaagc tatccgcgca agcgccgcct cgagctgctg tgctccgcgt
    11521 gggcgggcat cgacggccgc ctcgtgatcg ggggtgccga ccgcgcgaag ggggtcgcct
    11581 ggctgcgtcg ccacggggtg gaggagccgg ccggcgtcga gtgggcgggc gagctgccgc
    11641 gggcgcagtg gctcgaccgc gtgcaacgcg cgcgggtctt cctcaacgcc tcggcgtggg
    11701 aggacttcgg catcgccccg atggaggcgc tggcggctgg cacgcccgtg gtcacgcttc
    11761 cggcggcggg ctcgttcgag gcgctgccgc tgctgcgcga gctcgacccc cgtctggtga
    11821 cgaacgagga gagcttcgcg gatgcgatcc gcacagcgct cgcgctgacc gacgccgagc
    11881 gcgccgacta cgcgcgccgc gcgcgcgagc tgctcgcgcc ctacggcgag gacgcgatcg
    11941 cgcgggtggt ggccgagcag gtgctgccgg cgctggggat cgaacggtga aggtgctcgt
    12001 cgttcacaac cgctaccgcc agcgcggcgg cgaggagcgc gcagtcgatc tccagctcgc
    12061 cgcgctcgcc cgcgccggga tcgatcacga cgtgctgttc cgcgactcgg atggcgccgg
    12121 tcgcgcgcgg gccgcccgcg cgatgctgcg cggcggcgaa cgccccgagg aggtgggcga
    12181 tgccgtgcgg cgcctgggcg ccgacgtggt ccacgtgcac aacatgcacc cactgttcgg
    12241 accgcgctcg ctcgcggccg cgcgcgacgc gggtgcgcgc gtggtgctcc acctccacaa
    12301 cttccggctg ttctgctcga tcgcggtcgc gttccgcgac ggcgagacgt gctttcgctg
    12361 ccgccggcgc ttcacgctcc cgggcgccgt gctcgactgc cgcgagtcgg ccccggagtc
    12421 ggcggtctac gccaccgccc tcgcgcgcca ccagccgctg gtgttcgatt cggtcgaccg
    12481 cttcgtcacc ccgtcgcact tcgccgccgg tcagctcgcg cgcctcgggc tgccggccgg
    12541 ccggctcgac gtggtcccca actacctgcc ggcgggcgag ttcgcggagt cgagttccgc
    12601 gggcggcggg acgtacgccc tcgcgttcgg gcggctctcg cgggagaagg ggttcgagca
    12661 cgcgatcgcc gccgcggcgg cgtcgcacgt gccgctgcgc ctggccggtg acggcccgct
    12721 cgccggggag ttgcgcgagc gcgccgacgc gctcggcgcg ccggtcgagt tcctgggagc
    12781 ggtcgcgccc gagtgggtgc gcgagctgct gcgcggtgcg gcgatggcgc tagtgccgtc
    12841 ggtcgggccg gacgtgatgc cgttcgctgc gctcgaggcg atggccgccg gggtgccggt
    12901 ggtggcggcg cgcagcgggt cgctgccgga ggtggtggga ccgcaacgct gcgtcgcgcg
    12961 gcgcgacccg gccgccctcg caggggcgat gtccgcgctc tgggacgact ccggccaccg
    13021 cgccgacgag ggcgaggccg ccctcgcgcg cgcgcgcgag ctattcggcg aggagcgcta
    13081 cctgtgcgag ctgctcgcgg tgtacagagg ggccggcgga cccgttctga acacttagcg
    13141 gggctagagc accgttaacc gttcagaacg cgagtggccg cggttaggcc gccgtcgcgg
    13201 aggccgccgt tacgccggcg tcgccgcgcg ctgctcgcgc agccacgcca ccgtgtcgcg
    13261 cagcccgtcc gccagcggca cgcgcgcctc gaacccgatc agctcgcgcg ccttctcgac
    13321 cgacggccag cgccgctgca cgtccacgcg gtagctcggc agccgctcga gcaggaagtc
    13381 gtcgggtcgc tccccgcacg cctcccagac gagccgcgcg atctcggtca cgctcagctc
    13441 ctcggaggca gagatgttga agtcctcgtt ctccccggcg ggatggccca tggcggtcac
    13501 taccccgtcc gcgatgtcgt cgatgtgcgt cagcgtgcgc gtctggtcgc ccgagccgaa
    13561 gatctgcaac ggccgctgac cgtccagcac cttcttgatc aggtcgggca cgagatgcgc
    13621 gatccccggc tcgtcctccg gcatctcgcc gggcccgtag gcgttgaatg ggcggcagat
    13681 cgtgtacggc aggccgtgct cgtcggccag cgcgcggcag tagacctcgc ccgcgagctt
    13741 cgagaagccg tacgcggagt ccgggatgcc ggtgtcgcgt atgtgggcct cggtggtcgg
    13801 gaactcggtc gcgcgctcga agaccatcga gctcgacacg tacacgagcc gctcgacccg
    13861 gcggtggagc gccgcgcgga acaccgtgtt gaagagcccg ttgttgacct cgagcagcgt
    13921 gtgcggcagc ttgtggaagt tggcgatgcc gcccacgatc gcggcgaggt ggatcacgtg
    13981 cgtgcagccc tcggccgccg ccgcgcactc gccggggtcg agcaggtcgc cggtgtggac
    14041 ctcgcacgac tcgcgcatcc agccgggcac ctcgcgctgg tccgccacgc ggacctccca
    14101 ctccgggtcg cgaacgagcc ggcgcaccac ggcaccgccg atcgtccccg ccccgccggt
    14161 cacgagcact ctggccatgg cgtcgagggt acccgcggag cgccgctagc cgggcgcgcc
    14221 cgcgggctcg ggcaggcgcc gcggcgcctc tgccacgcgc gccacgtcct ccggcgcgcc
    14281 caccagcgcc ccgcccgggc actccgcgca cggcacctcg cgccgcggcc gggtcatgcc
    14341 cgcggccgac accaccccgc cgaggagcat cagcagtgcg gacgcggagg ccgcgaaccg
    14401 aaacgcctcc accgaggcgt cgccgaccgc gctctcgatc agctcccgct gcgtcgccgg
    14461 cagccccggc gggggcggct gcgggtcgag cgggcgcgcc tttgcgtcgg ccgcggccgc
    14521 gcgcgcggcc gggcttaccc cggacgcggc gtcgaggcgc tcgtcgagcg cggatccgaa
    14581 ccacgaggcc accagcgcgc ccaccgccgc gatcgccacg agccccgcca cgcgcgagat
    14641 cgcgttgttc accccggacg cgatcccggc gtgctccccg tcggcgtcgg ccagcaccga
    14701 ggccgtcagc ggcgcgacca ccatcgacat accgagcccg aacgcgagca ccgccgggac
    14761 cacgtcggtc agggacgagg tgtcctcgtc gagtcgcagc agcaggagga tcccggccgt
    14821 cgcgaccagc ggccccaggc tcatcagcgg gcgcgccccg atccggtcgg ccagcgcgcc
    14881 gaagcgcttg gccagcagga acatcacgat cgtcatcggc accagcgcga gccccgcctt
    14941 cgcggcgctg aagccggcca ccccctggag gaagatcggc atgaagaaga acgcgccccc
    15001 gagcccgccg tagatcagga acgtggccgc gttgccggcc gcgaagttgc gccgccggaa
    15061 cagcccgagc ggcagcatcg ggtcgggcga gcggcgctcc tggagcagga acagcgcgag
    15121 gcagatcgcg cccacgacga gcggcaggcc cacgagcgga tcgctccacc cgtacgacgg
    15181 ctgctcgatc agtgcgaaca ccacgccgct cagcccgacc gcgcacagca cggccccggg
    15241 cacgtcgacg tggcggtcga gccggtggtc gatcgcacgc ggcacgtgcc gggcgatcag
    15301 gtacagggtg gccgcgatcg gaagcaggcc gatcgcgaac accagccgcc acgagacggc
    15361 gtccacgagc acgccgccaa ggaacgggcc gatcagcgtg gacacgccgc tccacgccgt
    15421 ccacgagccg atcgccgccc cgcgctcgct cggttcgaag gtcgacacga tcaccgccag
    15481 tgcgctcggc acgagcagcg cgcccgccac gccctggagc gcgcgcgccg cgatcagcag
    15541 ctcggcggac ggcgcgacgg cgcagaggac ggacgtcgcg ccgaacccgc ccacacccag
    15601 cgcgaacacc cggcggcggc cgaacaggtc gctcagcgag ccgccgatca ggatcagcga
    15661 gccgagcatc agcaggtacg actcgatcac ccactgctgg gtggcgagcc cggcgtcgag
    15721 gtcgtcgctg atcgccggca gcgcgacgtt caccacggtc ccgtccacga agacgatcgc
    15781 cgacgagagg atgcaggcga ccagcgtgag ccgcttctgg cgcggcgtcg cggtcagcgg
    15841 ttgcgagcca gctcgcgtac cgccgcgaac acctcgcccg tccccgtcac gttccacggg
    15901 tcgacgataa cggcgtcgtg cggcagcagc gggaggatgt cctcgtactc cgagtggttc
    15961 gtggccacga cgacggcgtc ggcgcccgac agcgcgctct cgagcgtctc ggtgccctcg
    16021 gtgacgtacg ggtcgtggcg agcgacgtgg gccagctcgc gctcgagcag gcggatcagc
    16081 ttggcggcga gcgagtcgcg cacgtcgtcg gagtcgcgct tgaaggtgag cccgagcacg
    16141 gcgaccttgc agtcgcgcag cgagccgccc acgcgcttct tcagcccctc gaccaggaag
    16201 ttcgggaccg actcgtgcac gcgcgagacc gacagcagca tgcccggcgc gcgcgagcgc
    16261 tcctcggaga acgcgaagtc cttgcgcagg cacgtgcccg cggtgaggcc cggcgcggcc
    16321 atgccgccac gcgggtagtc gtggttgatc aggtcgatca cgccgaagac gttcgcgccg
    16381 tgctgctcgc actccatcat caggagattt ggcagggcga actgcgtgta gcggaggatg
    16441 ttggtccaga tcttcgcgag ctccgcctcg accgccgagg tctgcacgat ctcggtgccg
    16501 aacaccccga acagccgcgc ggcggcctcg ccggagccct cgccgacgcc gccgacgatc
    16561 gttgggaggg tggagatctc gtcgaggaag tggttcacgg cgatccgctc cggcacgtgc
    16621 gcgacgtgca cgtcctgacc gacggtgaag ccgcggcgct gctcgatgta gccggccacc
    16681 cactcggtgg tgccgggggc gacggtggag cgcaggatga tcgtctggcc ctcgcgaagc
    16741 agcggcagca gcgcgtcgat ggcgctgcgg atctgcgaga tgtcgatctc gatgtggacg
    16801 tgggtgggcg tgccgagcgt cagcactatg aactcggcct ccgaggcgtc ctggatgcgc
    16861 tgcgagagcc gcagcccgtc gccggcgacc acgcgctcga gcagctcctg cacgccggtc
    16921 tcgtcgaaag gcatctggcc ggcgcgcacg gtgtcgagca cgcgcggctc gcgatcgacg
    16981 ccgatcacgt ccaggccgcg gtcggcgaac gagagtgcca ggggaaggcc gatgcggccc
    17041 agccccacga tcgcgacgtc ggccggcatg ccgccgagcc tacccgcgcg ggtgcgagcg
    17101 tcgccgctcg ctgaggatct gccgcgtggc ggcagcgagg aagcgggggt tgtgcgagac
    17161 gtagcgcggc agcagccggc gcggctcctg cgcgatccgg tagctccact cgaggccgcg
    17221 gcgctgcatc cactcgggag cctggttgat acggccggcg aggaagtcga aggcggcgcc
    17281 gaccccgcac atgaccggca cgtcgagccg gtcgcgcatg tgggccatcc acttctcctg
    17341 cttcggcacg ccgatgccga cccacagcac gtccgggcgc gccgcgttga tctgccgcgc
    17401 gatctcctcc tcctcggcct ccgtcagcgg acggaacggc ggggagtagc cgcccacgat
    17461 gcgcatgccg gggttgacct tgtgcatgtt gagagcgagc tgggcgagcg agccctggtc
    17521 gcggccgccg tagagccaga cgcggtggcc gcggtcgcgg cagcgctcgt tgtagcggtg
    17581 catcagctcg ggcccgtaga cccggtcgtc gaggttctcg cccatcaggt tcgccgccca
    17641 aacgaccggc atgccgtcgg ggaccgtcag gctcgacccg aggagcgcgg cgcgcagctc
    17701 ggcgtcggtc tgggcctcca tgacggagtg caccggcgcc acgcagacgt aaccgcgctc
    17761 gcgccgcgcg atcatgccgt ccatcacgtc gagcgcctgg tcgtagtcgg tcatgccgat
    17821 cgggatgccg aggatctccc tggtccggac gtccctcggg gagggcagcg cggcggggta
    17881 gtcaaggggc gacgggctcc gcgtctccgg gaacgagatg agcgagcgcg gcagcggctc
    17941 gcccgtgatc ggctgaggct cgggggaaat cacttcgtaa tccggtgccc gaaggggagg
    18001 gcgtgcaaac cgtctacgac ccccgctcgg tcagtccttt cgagtctaga gaccaaggtg
    18061 tcctcagaac tcgcggccgg gcggaaagtt gcgcgcagtc aactcagacc ccgccggcgg
    18121 cgaacgccgc tatctgcgcg gccgtgatcg cacgggcgtc agcgccgccg cgccaggccc
    18181 ggaaccagtc cacggtcgcc gccagcgcct cgtcgaggtg ccagcgcggc tcccagccga
    18241 gcagctcccg ggcccgcgag gagtcgagcc gcagcaccgc ggtctccggc ggatgccccc
    18301 cgatcggggg gcatccatcc gtgcgccact cgagtccctc cccccagagc gccgccatgc
    18361 ggtcggcgat cgcgcgcacc ggcagctcgt cgccgggcgc ggggccgaag ttccaggcgt
    18421 ccgccgcgcc cggctcctcg ccgaggcgct gggccacctc gaggtagccg ctcagcgggt
    18481 tgagcacgtg ctgccagggg cgcaccgcgt ccgggttgcg caccatcagc gcctcgccgg
    18541 caagcgcggc gcgcaccgcg tccggcacga gccggtccgc cgcccagtcg ccgccgccga
    18601 tcacgttgcc cgcgcgcgcg gtggcgatcg cgggcccggc gcggccgaac gagtcccgcc
    18661 aggcggacgc cacgagctcg gcgcaggcct tgctgctcga gtacgggtcc gcgccgccga
    18721 gcgggtcgcc ctcgcggcag ggggcgctgt cggcgcgcgg ggcgtagcac ttgtcgctcg
    18781 tcaccacgac caccgcgcgc acgcccgagc ggaccgccgc gtcgagcacg ttcgcggtac
    18841 ccgtcacgtt ggtctcgtag gtgccgacgg gatccacgaa cgaggctcgc acgatcggct
    18901 gtgcggccag gtgtaggagc acgtcgggct cgagcgccgc caccgcgtca gccacggccg
    18961 cgcggtcgcg cacgtcgccg gtgcgcacgc cggccatgcc gtcgcccacc cgtgcggcgt
    19021 cgtagagcgc cgggtcggtc ggggcctcaa gcccgaagcc gctcacctcg gcccccagct
    19081 cctggagcca gagcgagagc cagctcccct tgaaccccgt gtgcccggtc acgagcacgc
    19141 ggcgcccgct ccagaagccc gcgtctactc ccacgtcttc cagggcgcct ggccggtgcg
    19201 ccacagctcc tcgaggtggt tgcggtcgcg caccgtgtcc atcgcccgcc agaagccctc
    19261 gtgccggtac gcgcgcagct cgccgtcgcg cgcgagccgc tcgaccggct cctcctccca
    19321 gacggtcgcg tcgccgtcga tgtagtcgcc cacgcgcggc gagaggacga agaacccgcc
    19381 gttcacccag gcgccgtcgc cgcgcggctt ctcgtggaag ctctcgaccc ggtcctcgtc
    19441 gaccgccatc gtgccccagc ggccgggcgg ctgcacggcg gtgacggtcg cgagccgccc
    19501 gtgcgagcgg tggtagtcga gcagcgcgcg cacgtcgagg tcgctcaccc cgtcgccgta
    19561 ggtgaacacg aactcgtcgc cgtccacgta gcgcagcacc cgcttcaggc gcccgccggt
    19621 ggcggtggcc gagccggtgt cgatcagcgt gaccttccac ggctcggccg tctcctgatg
    19681 gacctccatc gagtttccgg ccaggtcgaa ggtcacgtcg ctcgcgtgca ggtagtagtt
    19741 cgcgaaccac tccttgatca ccgcgccgcg gtagccgagg cagaccacga actcgtcgat
    19801 cccgccggcc gcgtacagcc ccttcatgat gtgccagagg atcgggcgct cgccgatctc
    19861 gaccatcggc ttgggcttca cgccggtctc ctcggcgatg cgcgtgccga ggcccccggc
    19921 caggatcacc gccttcacgg cagcgtgagc tccgacagga gcggggccgc gacgtcgcgc
    19981 tcggaagcga tgagctcgac gtccggccag tcgatgccga cctccgggtc gtcgtagcgg
    20041 aagccgctct cgcgcgccgg gtcgtagtag tcgccgcact tgtagagcac gtccgcgacc
    20101 tcgctcgtca cgcagaagcc gtgcgcgaag ccgtcgggca cgtagagctg gtgcagggtc
    20161 tcgtcgttca gctcgaacgc ctcccagcgc ccgaacgacg gcgacccgcg gcggatgtcc
    20221 acgagcacgt cgacgatcga gccgcgcgcg cagcgcacca gcttcgactg cccgggctgg
    20281 aagtgcatcc cgcgcaccac gccgagccgc gagcgcgagt ggttgtcctg cacgaactcg
    20341 tcgccgacac cgaggtcggc gagcacgttg cggcggtagg tctcctggaa gaagccgcgc
    20401 gcgtcgccat gcaccttggg cgcgatcagg accggcccct cgagcttcgt ggcgagggcc
    20461 tgcactatcc gtagaaccgg gagatggtca gcaattgagc gaacagcgta tgcgcgaacg
    20521 cggccagcac gatgaccgcc ccgaacgcgg gcccgaaccg gcgcccgggg gcgcgcgcgg
    20581 ccaggccgag cagcaccgcg tagagcggga gcaggggcag caggtagcgc gcctggcgga
    20641 agttcggccc gccgccgagg taggccggga agtccgcatg gccgatcacg agcatcaccc
    20701 cggcggccat cagggtgtag atcgccaggt cgggcaggcg gcgcaggagc ggccggccgc
    20761 gg
//
LOCUS       DASVPL010000042        12145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_200133, whole genome shotgun sequence.
ACCESSION   DASVPL010000042 DASVPL010000000
VERSION     DASVPL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12145
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200133"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VF517_02415"
     CDS             complement(<1..1589)
                     /locus_tag="VF517_02415"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX8051815.1"
                     /translation="MRRATLAAAAVLAVLAAAPGAFAAEYEWNEGNDIVTSPDGRNVY
                     AAGATTLSFRADPEGGALTLIGHTEPGADRPQMAISPDGRHVYVGRGRNYPSGAILVM
                     SRDAESGLLTHEETFTGADPGTAAVGPVADLEMSVDGRQLYVAESNPDLVAVYERNVE
                     TGALTQRQVLYRGADLPVAYALDLAGSPDGRHIYVAGSNLAILRRDPTTGLLSAAGTA
                     QDNGSDFAVAVSADGGRVYGGMTNVDSWRRDPEGGALEHIGHGEFVAQSCGACDSGRM
                     IVASPDGRVYSSSPPDSALLEARPADDGLVLERRYVDGQDGVRGLGHPNAMAWSANGR
                     FAYVASARYIGSGGSYYSSSGNEGAVAAFRRTGAGLDVVGAHGPGLQVDEAPQKSYRP
                     PAVTINDGALYTNDPNVEVTVSNDPGAASLRLSNTGDFAAAPALRIATAEQTFPWRLA
                     DGGSERSVRHVHVRFTPLRREEVRISDDIILDQRPPELITARIRGTRLLLKARDNRSG
                     VKRVQVTTSRKRPGKARKFKG"
     gene            complement(1586..3301)
                     /locus_tag="VF517_02420"
     CDS             complement(1586..3301)
                     /locus_tag="VF517_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX8051816.1"
                     /translation="MSNYLPPGGFGHRMLATARSLCGAIALAVLALALSSAPAGASST
                     RCCVPFQQPGQLAMSDDGQHLYVADNQSTLALRRDPASGALTLIDSYDGGGGPLELSP
                     DGRHLYVISDAPTMGPTVHAFKRDAVTGALSYVGRWSQTRGGGPRDIEFRDDRTAYMT
                     DELRDALMVFDRDPATGRLDMRSELRNGQNGVEGLATPDGIEVGDGWLYVNQRAGPHS
                     IGVFRLAENGDPRSEPDPGCDCSGYRDLELMPDGRRLLAGPFGPYLYDRDPATGRLAD
                     RPQTNAVAAGGDELADGTLAIDPGGAVVYGTDWWANRLNQYDHGGDGIALRRSYYEGR
                     DGQGINRPRSVVLSPDGRHLYLAAGSLGGGEAGTVSAFRRDPASGELSFASLYRGPVF
                     DGRPHHLEGQPPRLLINGGDEYTNDPDVMLSVEDIDRGTFALEVSNDGGFKKSERRTI
                     DETNAYPWTLASSGPERLPKTVYARLSGMSRFGGQPITDEIVLDERAPTIVFARQAKR
                     RLDLKARDDLSGVSHVQATRNPKRPGRWRRFAKSVSVPGGRGRIHVRVRDRANNRSRW
                     RVVGR"
     gene            3383..5005
                     /locus_tag="VF517_02425"
     CDS             3383..5005
                     /locus_tag="VF517_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8051817.1"
                     /translation="MEAATQTSPATNGAGPGRPELESFNPATGERIGAVPKLDPGEIQ
                     AIVDDVAEVQPFWAQLPLSDRARYMRRTAQVVIDNLEDLTTLLSREQGKPKAESYAME
                     LVPTIDALHWIADNGPRILAPEPVRGVPVFAKQKSHHFSYEPLGVVGVIAPWNYPWSI
                     PFGEVAMALMAGNGVVLKPASLTPLIGERIRWAFERAGLPEGIVRTIHGGGAVGNALV
                     ESTASKIFFTGSVEIGRNVGIACAERMKGCVLELGGKDSAIVLGDANLDHAVAGTLWG
                     SFANAGQTCSGIERAYVMREVADRYVAGVVEGARRLRVGDPLRPDTEIGPMVSQDQYD
                     IVVELVDDAVANGAKLECGGPVEAAGLSGSFYAPAVLTGVTPDMRIMREEIFGPVVTV
                     TTVDSEDEAIALANDSEFGLGNSVWTCDRAKGERIAERLESGMVWINEHMYTHGLCGC
                     AWGGVKNSGIGRAHSQHGFYECVNVKQVAWDPSLWRDFWWHPYDESNSRGLHAAAQLL
                     YGRDEDKAAALRRGARPLARLARKSLKGAFRR"
     gene            complement(5002..5766)
                     /locus_tag="VF517_02430"
     CDS             complement(5002..5766)
                     /locus_tag="VF517_02430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX8051818.1"
                     /translation="MPSPGYFTDDSMLRIVHRERAIALSGPRALLMQAAHPVAVKGLL
                     AHSSALDEPYDRLARTAEVMSTIGFGSREDAERVTARVRAMHRKANVDKPDLLLWVLF
                     SLVDSGIVVYRMYVRGMDDEQQAAYWEDYKVVGELFGLSRAEMPDTLADLREYKREML
                     EGGTLTVNDWARTRAREIVLEPPVPIVAQPLVELVNFITISLLPGEIRRQYRFSPLPP
                     APLRRALVAGGAEYVKRAVIPFMPDRLRLVPAARAA"
     gene            5804..6541
                     /locus_tag="VF517_02435"
     CDS             5804..6541
                     /locus_tag="VF517_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX8051819.1"
                     /translation="MPAQAYAGKECVCQFRRGICDQTCVRDMLETPFYIACLRLKGRR
                     CVVVGGGEIGLEKVEGLLACDGAVTLIAPEAGPELEQLAREGSIAWERREYETGDLEA
                     TFIAIAATDDTDVNIRVYEDAERRAMLVNVVDVPPLCNFILPAIVRTGPLAIAISTAG
                     ASPALAKRIKSEIAEQYGEPYARLAEILNDARGWAKATLPTYQDRKVFFESIVNGEPD
                     PVELLRAGDEGAVRDLVAAAQRTAVPA"
     gene            6538..7005
                     /gene="moaC"
                     /locus_tag="VF517_02440"
     CDS             6538..7005
                     /gene="moaC"
                     /locus_tag="VF517_02440"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX8051820.1"
                     /translation="MAGLSHIDDEGRARMVDVGGKPPTDRRARARVTVRMSPSTARLV
                     ETGSGPKGDVVAVARIAGIQAAKRTSELIPLCHPLALTYVDVAIAVGEDSVTVEAETR
                     TTAETGVEMEAMTAASVAALTIYDMVKGVERGVAIEELVLLEKTGGKEDWRRP"
     gene            7002..7469
                     /locus_tag="VF517_02445"
     CDS             7002..7469
                     /locus_tag="VF517_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX8051821.1"
                     /translation="MTRAAVLTISTTLSAGEGEDVSGRELVRLCEEAGLDVSHEVLPD
                     ERAAIVEALTRLADREGCRFVFTTGGTGLTPDDVTPEATLEAIDREAPGFAEAIRVES
                     REHTPLGILTRGVSGVRGRTLIVNFPGSPKAIGQSWPVVAPTLKHAAQTLERG"
     gene            7462..8127
                     /locus_tag="VF517_02450"
     CDS             7462..8127
                     /locus_tag="VF517_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8051822.1"
                     /translation="MADAIALDGLERRYGDRVVLHDLTLAVPAGATLTLFGANGAGKT
                     TLLRILATLLRPHDGTAEVLGSKLPDDAWRVRGRVGLLGHEPLLYPQLSARENLTLHA
                     RLHGVPHERIDAVLDAVGLRERAGDPVDELSKGMVQRLAAARAVLHDPPLLLLDEPRA
                     GLDPAAAEQLEPLIGRSSGRTRVLVTHDVDRGLEEGDVVVGLRDGRAEPCADAAAVRR
                     LYG"
     gene            8124..8798
                     /locus_tag="VF517_02455"
     CDS             8124..8798
                     /locus_tag="VF517_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX8051823.1"
                     /translation="MIGHAAAILRKDLRIELRTRESVPAMALFSVSAFVLFHFGLHRD
                     AIEGDLASGVLWVTLLFASVLGVNRLFAGEHEGGALDGLLLAPIDRTAIYVAKASALF
                     LYLVLLELVAVPAFALLLLGPGLGAALPELLLILLLADLGLAAVGALVAGLAAEARSR
                     DLIVPLLLLPLLVPLLIAAARATEPLLATPAGPEDLAGWLGFMALYDTVFLLVAVAVF
                     DFLLED"
     gene            8809..9576
                     /gene="ccsA"
                     /locus_tag="VF517_02460"
     CDS             8809..9576
                     /gene="ccsA"
                     /locus_tag="VF517_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX8051824.1"
                     /translation="MRSRPLFGNGLRALSITSAVLLAAGFGLAFFYAPLDADQGIIQK
                     IFYVHVPMAIVALCGFVAGGIMAIQHLRTGDRSWDMRSYVAIHMSIVLGVGVLVTGGI
                     WGKASWGHWWEWDEPVLVSFLIVFLLYCCYQPLRFSIADPERQGRYASVFAIAAGAFV
                     PINFLAVRMATQELHPRVLSETGGSMPGEMRLAFFVCLAGMALLFATLWKLELSSKNA
                     SEQLKRLRMRLEETPFEAPGGATARPPAVPARPSGVE"
     gene            9579..9746
                     /locus_tag="VF517_02465"
     CDS             9579..9746
                     /locus_tag="VF517_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051825.1"
                     /translation="MDLLPLALTGRQYVAAAYLVFLALVLLYVVIISSKIARLEREVN
                     TLGELADKRPR"
     gene            9743..11083
                     /gene="hemA"
                     /locus_tag="VF517_02470"
     CDS             9743..11083
                     /gene="hemA"
                     /locus_tag="VF517_02470"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX8051826.1"
                     /translation="MSAAILAIGASHKTAPLALRERLALPEGRAAHVLSEIVSHPEIR
                     EAVAISTCNRTELYMVVGDPVEAESAALGCVSRQVGMRPTELVGSLYSHRDGEAVRHL
                     FEVAAGLDSMVVGEAEVLGQVKRAYELALVEGATGPISNRLFRDAIATGKRVRTETAL
                     GRAHDSVSSVAVDLARSLLGDLAARSVLMIGAGETAELTAKALHQRGADTIFVANRRH
                     DRAIGLAQRFGGSAVRFEDLPARLLDTDIVVSSTASPHQIVGADELVEVMGQREGRPL
                     LIIDIAVPRDIDASVRDVPGVTLYDMDDLQREAARNLSVQSAEATRARALVEDEAARF
                     ERWSASLDVVPTIAALRERGEEIVAQVLRENESRWVGLGEEDRARVELMARAVVSRLL
                     HEPTLRLKAAEGDESYVFVQALRELFAIDAGELEAPPSAAEVTSLDERRRRQSR"
     gene            11134..>12145
                     /gene="hemC"
                     /locus_tag="VF517_02475"
     CDS             11134..>12145
                     /gene="hemC"
                     /locus_tag="VF517_02475"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX8051827.1"
                     /translation="MVAGWLDADVELIEITTSGDERTAGGAPGPAARTGRPGPPARAG
                     RPGPPARAGRVASPPTDKSRFVKEIEEALLRGDVDLAVHSAKDVPGELPEGLAIVGVP
                     ERADPRDSLCGAASLDALAEGARVGTSSLRRRAQLLAARPDLRVEDVRGNVDTRLRRL
                     ADGDYDALVLAAAGLARLGRDEGSAIPAATMVPAPGQGCLALEARAGDAEMAVLAAAL
                     TDRAALTALTAERALVGELEATCNTPLAAYAELVTSAAPGGAAPPPGEAAAPGEAAAP
                     GEAAAPGEAAAPGEAAAPGGAGAGTLRLTAFAGLPDGSHWITDVLEGDAADPAALGRA
                     MAE"
BASE COUNT         1540 a         4295 c         4607 g         1703 t
ORIGIN      
        1 ctgcccttga acttgcgcgc cttgccgggc cgcttgcgcg aggtcgtgac ctgcacgcgc
       61 ttcacgccgg agcggttgtc gcgcgccttc agcaggaggc gcgtgccgcg gatgcgcgcg
      121 gtgatcagct cgggcgggcg ctggtcgagg atgatgtcgt cggagatgcg gacctcctcg
      181 cgccggagcg gcgtgaagcg cacgtgcacg tgcctgaccg agcgctccga cccgccgtcg
      241 gccagccgcc acgggaaggt ctgctcagcc gtcgcgatcc gcagcgcggg cgccgccgcg
      301 aagtcgccgg tgttcgacag gcgcaggctc gcggcgcccg ggtcgttgga caccgtcacc
      361 tcgacgttcg ggtcgttcgt gtagagcgcg ccgtcgttga tggtcaccgc cggcggccgg
      421 taggacttct gcggtgcctc gtcgacctgg aggccggggc cgtgcgcgcc cacgacgtcg
      481 agccccgcgc ccgtgcgccg gaacgcggcg accgcgccct cgttaccgct gctcgagtag
      541 tagctgccgc cgctgccgat gtaccgggcc gacgccacgt aggcgaagcg gccgttcgcg
      601 ctccacgcca tcgcgttcgg gtggccgagc ccgcgcactc cgtcctggcc gtcgacgtag
      661 cggcgctcga ggacgaggcc gtcgtcggcc ggccgcgcct cgagcagcgc ggagtccggg
      721 ggcgacgagc tgtacacgcg gccgtcggga gacgccacga tcatccgtcc cgagtcgcac
      781 gccccgcagc tctgcgcgac gaactcaccg tggccgatgt gctcgagtgc gccgccctcg
      841 gggtcgcggc gccagctatc gacgttcgtc atgccgccgt acacgcggcc gccgtctgcc
      901 gagaccgcga cggcgaagtc gctgccgttg tcctgcgccg tgccggccgc gctgaggagg
      961 cccgtggtgg ggtcgcgccg caggatcgcg aggttgctgc ccgccacgta gatgtggcgg
     1021 ccgtcgggcg aaccggcgag gtcgagcgcg taggcgaccg gcaggtcggc gccccggtag
     1081 agcacctggc gctgggtgag cgcgccggtc tcgacgttgc gctcgtagac ggcgacgagg
     1141 tcggggttgc tctcggccac gtagagctgg cggccgtcga ccgacatctc cagatccgcg
     1201 accgggccga ccgcggccgt ccccggatcg gccccggtga acgtctcctc gtgcgtgagc
     1261 aggccgctct cggcgtcgcg cgacatcacc aggatcgctc ccgacgggta gttgcggccg
     1321 cgccccacgt acacgtggcg accgtcgggc gagatcgcca tctgcggccg gtcggcgccg
     1381 ggctcggtgt gcccgatcag cgtgagcgca ccgccctcgg ggtcggcgcg gaagctgagg
     1441 gtggtggcac ctgctgcata cacgttgcgc ccgtccggtg aggtgacgat gtcgttgccc
     1501 tcgttccact cgtactccgc ggcgaaggcg ccgggagcgg ccgcgagcac ggcgagcacc
     1561 gcggcggctg cgagcgtggc gcgcctcatc gcccgaccac ccgccagcgc gagcggttgt
     1621 tcgcgcggtc gcgcacgcgc acgtggatcc gcccgcggcc gccagggacg ctgacgctct
     1681 tcgcgaagcg cctccacctc ccgggccgct tcgggttgcg tgtggcctgc acgtgcgaga
     1741 cgccggagag gtcgtcgcgg gccttcaggt cgagccgcct cttcgcctgc cgcgcgaaca
     1801 cgatggtcgg cgcccgctcg tccaggacga tctcgtcggt gatcggctgg ccgccgaagc
     1861 ggctcatccc gctcaggcgg gcgtagacgg tcttcgggag ccgctccggg ccgctgctcg
     1921 cgagcgtcca cgggtaggcg ttggtctcgt cgatcgtgcg ccgctcgctc ttcttgaagc
     1981 cgccgtcgtt cgagacctcg agcgcgaagg tgccgcggtc gatgtcctcg acgctcagca
     2041 tcacgtcggg gtcgttcgtg tactcgtcgc cgccgttgat cagcagcctc ggtggctggc
     2101 cctcgaggtg atgcgggcgc ccgtcgaaga ccgggcccct gtacagcgac gcgaacgaca
     2161 gctctccgct ggcggggtcg cgcctgaagg cgctcacggt gccggcctcg ccgccgccca
     2221 gtgacccggc ggcgaggtac aggtgcctgc cgtcggggga gaggacgacc gagcgcggcc
     2281 ggttgatccc ctggccgtcg cggccctcgt agtaggaccg gcgcagggcg atcccgtcgc
     2341 cgccgtggtc gtactggttg agccggttcg cccaccagtc ggtcccgtag acgacggcgc
     2401 cgccgggatc gatcgcgagc gtcccgtcgg cgagctcgtc gccgcccgcc gcgacggcgt
     2461 tcgtctgcgg gcggtcggcc aggcggccgg tggccgggtc gcggtcgtac aggtacgggc
     2521 cgaacgggcc ggccagcagg cgccgcccgt cgggcatcag ctcgaggtcg cggtagccgg
     2581 agcagtcgca gcccgggtcg ggctcggatc tcgggtcgcc gttctcggcc aggcggaaca
     2641 cgccgatcga gtggggcccg gcgcgctggt tcacgtagag ccagccgtcg ccgacctcga
     2701 ttccgtcggg cgtcgcgagg ccctccacgc cgttctgccc gttgcgcagc tcgctgcgca
     2761 tgtcgagccg gccggtggcg ggatcgcggt cgaagaccat cagcgcgtcg cgcagctcgt
     2821 cggtcatgta tgcggtgcgg tcgtcgcgga actcgatgtc gcgcggacct ccgcccctcg
     2881 tctggctcca gcgcccgacg tagctgaggg cgcccgtgac ggcgtcgcgc ttgaacgcgt
     2941 gcacggtggg gcccatggtg ggcgcgtcgg agatcacgta caggtggcgg ccgtcgggcg
     3001 agagctcgag cggcccgccg ccgccgtcgt aggagtcgat gagtgtgagc gcgccgctgg
     3061 cgggatcgcg gcgtagcgcg agggtgctct ggttgtccgc gacgtagagg tgctgcccgt
     3121 catccgacat cgccagctgg cccggttgct ggaacggcac gcagcagcgc gtgctcgagg
     3181 cgcccgccgg ggcggagctc agcgcgaggg cgaggacggc gagggcgatt gccccgcaca
     3241 gggatcgggc cgtggccagc atgcggtgtc caaacccgcc tgggggcagg tagttgctca
     3301 tcgtaggtgc gtacgtgcca aacggcaatg ggccgaggat ccccggtagg gcacaatggt
     3361 ccggaccagc gaggaggcag acgtggaggc agctacgcag accagcccgg ccaccaacgg
     3421 cgccgggccc gggcggccgg agctcgagtc gttcaacccg gccaccggcg agcgcatcgg
     3481 cgcggtgccg aagctcgacc cgggcgagat ccaggcgatc gtcgacgacg tggccgaggt
     3541 ccagccgttc tgggcgcagc tcccactgtc ggaccgcgcg cgctacatgc gccgcaccgc
     3601 gcaggtggtc atcgacaacc tcgaggacct caccacgctg ctctcgcgcg agcagggcaa
     3661 gccgaaggcc gagtcgtacg cgatggaact cgtgccgacg atcgacgccc tgcactggat
     3721 cgccgacaac ggcccgcgga tcctcgcgcc cgagccggtc cgcggggtgc ccgtcttcgc
     3781 caagcagaag agccaccact tctcctacga gccgctgggc gtcgtgggcg tgatcgcccc
     3841 gtggaactac ccgtggtcga tcccgttcgg cgaggtcgcg atggcgctga tggcgggcaa
     3901 cggcgtggtg ctcaagccgg cctcactcac gccgctgatc ggcgagcgga tccgctgggc
     3961 gttcgagcgc gccgggctcc cggagggcat cgtgcggacg atccacggcg gcggggcggt
     4021 cggcaacgcg ctggtcgagt cgaccgcgtc gaagatcttc ttcaccggct ccgtcgagat
     4081 cggccgcaac gtgggtatcg cgtgcgccga gcggatgaag ggctgcgtgc tcgagctggg
     4141 cggcaaggac tcggcgatcg tgctgggcga cgcgaacctc gaccacgccg tggccggcac
     4201 gctgtggggc agcttcgcga acgcggggca gacctgctcg gggatcgagc gcgcctacgt
     4261 gatgcgcgag gtggcggacc gctacgtggc cggcgtcgtc gagggcgccc ggcggctgcg
     4321 agtcggtgac ccgctgcggc cggacaccga gatcggcccg atggtctcgc aggaccagta
     4381 cgacatcgtg gtcgagctcg tggacgacgc ggtggcgaac ggcgcgaagc tcgagtgcgg
     4441 cgggccggtc gaggccgccg ggctgagcgg ctcgttctac gcgcccgcgg tgctcaccgg
     4501 cgtgacgccc gacatgcgga tcatgcgcga ggagatcttc ggaccggtcg tgacggtgac
     4561 gaccgtcgac tccgaggacg aggcgatcgc gctcgcgaac gactccgagt tcgggctcgg
     4621 caactccgtg tggacgtgcg accgcgcgaa gggcgagcgg atcgcggagc ggctcgagag
     4681 cgggatggtc tggatcaacg agcacatgta cacgcacggg ctgtgcggct gcgcgtgggg
     4741 cggcgtgaag aactcgggca tcggacgcgc gcactcgcag cacggcttct acgagtgcgt
     4801 gaacgtgaag caggtcgcct gggacccgtc gctgtggcgc gacttctggt ggcaccccta
     4861 cgacgagtcg aactcgcgcg gcctgcacgc cgcggcgcag ctcctctacg ggcgtgacga
     4921 ggacaaggcc gccgcgctgc ggcgcggggc gcggccgctg gcgcggctgg cgaggaagtc
     4981 gctgaagggc gcgttcaggc gctaggccgc gcgcgccgcc ggcaccagcc gcagccggtc
     5041 cggcatgaac gggatcaccg cgcgcttgac gtactcggcg ccgcccgcca cgagcgcgcg
     5101 gcgcagcggc gccgggggca gcgggctgaa gcggtactgc ctgcggatct ccccaggcag
     5161 cagcgagatc gttatgaagt tgacgagctc gaccagcggc tgcgcgacga tcggcaccgg
     5221 cggctcgagc acgatctccc gcgcgcgcgt gcgggcccag tcgttcaccg tgagcgtgcc
     5281 gccctcgagc atctcgcgct tgtactcgcg caggtcggcg agcgtgtcgg gcatctcggc
     5341 gcggctcagc ccgaacagct cgcccacgac cttgtagtcc tcccagtagg cggcctgctg
     5401 ctcgtcgtcc atcccgcgca cgtacatccg gtagaccacg atcccgctgt ccacgaggct
     5461 gaacaggacc cagaggagga gatcgggctt gtcgacgttc gccttgcggt gcatcgcgcg
     5521 cacccgcgcc gtgacccgtt cggcgtcctc gcgcgagccg aagccgatcg tgctcatcac
     5581 ctcggcggtg cgcgccaggc ggtcgtacgg ctcgtcgagc gcgctcgagt gcgccagcag
     5641 ccccttgacc gcgacggggt gcgccgcctg catcaggagg gcgcgcggtc ccgacagtgc
     5701 gatcgcccgc tcgcggtgca cgatgcggag catcgagtcg tccgtgaagt agccaggcga
     5761 gggcatctca cagagggtac ctgtccgtac actcgattgg accatgccag cccaggccta
     5821 cgccggtaag gagtgcgtct gccagttcag gcgcggcatc tgcgatcaga cctgcgtccg
     5881 cgacatgctc gagacgccgt tctacatcgc gtgcctgcgg ctcaaggggc gccgctgcgt
     5941 ggtggtgggc gggggtgaga tcgggctcga gaaggtcgag ggcctgctcg cctgcgacgg
     6001 cgccgtgacg cttatcgcgc ccgaggcggg gccggagctc gagcagctcg cccgcgaggg
     6061 ctcgatcgcg tgggagcgcc gcgagtacga gacaggcgac ctcgaggcga ccttcatcgc
     6121 gatcgccgcc accgacgaca ccgacgtgaa catccgcgtg tacgaggacg ccgagcggcg
     6181 cgcgatgctc gtgaacgtgg tggacgtgcc tccgctctgc aacttcatcc tgccggcgat
     6241 cgtgcgcacc ggcccgctcg ccatcgcgat ctcgaccgcg ggggcgagcc ccgcgctggc
     6301 caagcgcatc aagtccgaga tcgccgagca gtacggcgag ccgtacgcgc gcctggccga
     6361 gatcctgaac gacgcccgcg gctgggcgaa ggcgactctc ccgacctacc aggaccgcaa
     6421 ggtcttcttc gagtcgatcg tcaacggcga gcccgacccg gtcgagctgc tgcgagcggg
     6481 cgacgagggg gccgtccgcg atctggtcgc ggcggcgcag cggacggccg tccccgcata
     6541 gcgggcctct cccacataga cgacgagggc cgcgcgcgga tggtggacgt cggcggcaag
     6601 cccccgaccg atcgccgcgc ccgcgcccgg gtgacggtgc gcatgtctcc ctccacggcg
     6661 aggctggtgg agacgggctc cggcccgaag ggggacgtgg tcgcggtcgc ccgcatcgcc
     6721 ggcatccagg ccgcgaagcg cacctccgag ctgatcccgc tgtgccaccc gctggcgctg
     6781 acgtacgtgg acgtcgccat cgccgtgggg gaggactcgg tgaccgtcga ggccgagacg
     6841 cgcacgacgg ccgagaccgg cgtcgagatg gaggccatga ccgcggcctc ggtcgcggcg
     6901 ctgacgatct acgacatggt caagggggtc gagcgcgggg tcgcgatcga ggagctggtc
     6961 ctgctggaga agacgggggg caaggaggat tggcggcgac cgtgacgcgc gcggcggtgc
     7021 tcacgatctc gacgaccctc tccgccggcg agggcgagga cgtctcggga cgggagctcg
     7081 tgcggctgtg cgaggaggcc ggcctcgacg tctcgcacga ggtgctgccg gacgagcgcg
     7141 ccgcgatcgt ggaggcgctc acgcggctgg ccgaccgcga gggctgccgg ttcgtgttca
     7201 ccaccggcgg gaccgggctc acgcccgacg acgtgacgcc ggaggcgacg ctcgaggcga
     7261 tcgaccgcga ggcgccgggc ttcgcggagg cgatccgcgt ggagtcgcgg gagcacacgc
     7321 cgctcggcat cctcacgcgc ggtgtctcgg gcgtgcgcgg gcggacgctg atcgtgaact
     7381 tcccgggctc gccgaaggcg atcgggcagt cgtggccggt ggtcgcgccg acgctgaagc
     7441 acgcggcgca gacgctcgag cgtggctgac gcgatcgcgc tcgacgggct cgagcggcgc
     7501 tacggcgacc gcgtggtcct ccacgacctc accctcgcgg ttcccgccgg cgcgacgctg
     7561 acgctgttcg gcgcgaacgg ggcgggcaag acgaccctgc tgcgcatcct cgcgaccctg
     7621 ctgcggcccc acgacgggac cgccgaggtg ctcggctcca agctgccgga cgacgcatgg
     7681 cgcgtgcgcg ggcgcgtggg gctcctgggc cacgagccgc tgctctaccc gcagctctcc
     7741 gcgcgcgaga acctgaccct ccacgcgcgg ctgcacggcg tgccgcacga gcggatcgac
     7801 gccgtgctcg acgccgtcgg cctgcgcgag cgcgctggcg acccggtcga cgagctgtcg
     7861 aaggggatgg tgcagcgcct cgccgccgca cgcgcggtcc tgcacgaccc gccgctgctg
     7921 ctgctcgacg agccgcgcgc ggggctcgac ccggccgcgg ccgagcagct cgagccgctc
     7981 atcggccgct cgtctgggcg aacccgcgtg ctggtgacac acgacgtcga ccgcgggctc
     8041 gaggagggcg acgtggtcgt cgggctgcgc gacgggcggg cggagccgtg cgcggacgcg
     8101 gcggcggtgc ggaggctcta cgggtgatcg ggcacgccgc cgccatcctc cgcaaggacc
     8161 tccgcatcga gctgcgcacg cgcgagtccg tgccagccat ggcgctcttc agcgtcagcg
     8221 cgttcgtgct cttccacttc ggcctgcacc gggacgcgat cgagggcgac ctcgcgtcgg
     8281 gcgtcctgtg ggtcacgctg ctgttcgcga gcgtgctcgg tgtgaaccgt ctgttcgcgg
     8341 gcgagcacga gggcggcgcg ctcgacgggc tgctgctggc cccgatcgac cgcaccgcga
     8401 tctacgtggc caaggcgagc gcgctgttcc tctacctcgt gctgctcgag ctcgtggccg
     8461 tgccggcgtt cgcgctgctc ctgctcgggc ccggcctcgg ggccgccctc ccagagctcc
     8521 tcctcatcct gctgctggcc gacctcggcc tcgccgcggt gggcgcgctg gtcgccggcc
     8581 tggcggctga ggcgcgctcg cgcgacctga tcgtgccgct gctcctgctg ccccttctcg
     8641 tgccgctgct gatcgccgcc gcgcgggcca cggagccgct gctggccact ccggccgggc
     8701 ccgaggacct cgccggttgg ctgggtttca tggctctcta cgatacggtc ttcctgctgg
     8761 ttgcagtagc cgtcttcgac ttcctcctag aggactgagg ggctgacagt gcggtccaga
     8821 ccgctgttcg gcaacgggct tcgcgccctc tcgatcacct ccgccgtact gctcgcggcg
     8881 ggcttcgggc tggcgttctt ctacgccccg ctcgacgccg accaggggat catccagaag
     8941 atcttctacg tgcacgtgcc gatggcgatc gtcgcgctct gcggcttcgt ggcgggcggc
     9001 atcatggcca tccagcacct gcgtaccggc gaccggagct gggacatgcg ctcgtacgtg
     9061 gcgatccaca tgtcgatcgt gctcggcgtg ggcgtgctgg tcaccggggg gatctggggc
     9121 aaggcgtcct ggggccactg gtgggagtgg gacgagccgg tgctggtctc gttcctgatc
     9181 gtgttcctcc tgtactgctg ctaccagccg ctgcgcttct cgatcgccga ccccgagcgc
     9241 cagggccgct acgcgtcggt cttcgcgatc gccgcgggcg cgttcgtgcc gatcaacttc
     9301 ctcgcggtgc ggatggccac gcaggagctc cacccgcgcg tgctctcgga gaccggcggc
     9361 agcatgccgg gcgagatgcg gctggcgttc ttcgtctgcc tcgccggcat ggcgctgctg
     9421 ttcgcgacgc tgtggaagct cgagctgtcg tcgaagaacg cgtcggagca gctcaagcgg
     9481 ctgcggatgc ggctcgagga gacgccgttc gaggccccgg gcggcgccac ggcacgcccg
     9541 ccggcggtcc cggcgcgacc gagcggggtg gagtgaaggt ggacctgctt ccgctcgcgc
     9601 tcaccggccg ccagtacgtg gcggcggcgt acctcgtctt cctcgccctc gtgctcctgt
     9661 acgtggtgat catcagctcg aagatcgcgc gcctcgagcg cgaggtgaac accctcggcg
     9721 agctggccga caagaggccg cgatgagcgc cgcgatcctc gccatcggcg cctcgcacaa
     9781 gaccgctccg ctggcgctgc gcgagcggct cgcgctgccg gagggccgcg cggcgcacgt
     9841 gctgtccgag atcgtgtcgc acccggagat ccgcgaggcg gtggcgatct cgacctgcaa
     9901 ccgcaccgag ctgtacatgg tcgtgggcga tcccgtcgag gccgagagcg ccgcgctcgg
     9961 ctgcgtctcg cgccaggtcg ggatgcggcc caccgagctg gtcggcagcc tctactcgca
    10021 ccgcgacggc gaggccgtgc ggcacctgtt cgaggtggcg gcggggctcg actcgatggt
    10081 cgtgggcgag gccgaggtgc tcggccaggt caagcgcgct tacgagcttg cgctcgtcga
    10141 gggggccacc gggccgatct cgaaccgcct gttccgcgac gcgatcgcga ccggcaagcg
    10201 ggtgcggacc gagaccgcgc tcgggcgcgc gcacgactcg gtgtcgagcg tggcggtgga
    10261 cctcgcgcgc tcgctgctcg gcgacctcgc cgcgcgcagc gtgctgatga tcggcgcggg
    10321 cgagaccgcg gagctcaccg cgaaggcgct gcaccagcgc ggcgccgaca cgatcttcgt
    10381 ggccaaccgc cgccacgacc gcgcgatcgg cctcgcgcag cgcttcggcg gcagcgcggt
    10441 ccgcttcgag gacctgcccg cgcggctgct ggacaccgac atcgtggtga gctcgacggc
    10501 gtcgccgcac cagatcgtcg gcgctgacga gctggtcgag gtcatgggcc agcgcgaggg
    10561 caggccgctg ctgatcatcg acatcgcggt gccgcgcgac atcgacgcgt cggtgcgcga
    10621 cgtcccgggc gtcacgctct acgacatgga cgacctccag cgcgaggccg cgcgcaacct
    10681 gtcggtgcag tccgcggagg ccacgcgcgc gcgggcgctg gtcgaggacg aggcggcgcg
    10741 cttcgagcgc tggtccgcga gcctcgacgt ggtgccgacg atcgcggcgc tgcgcgagcg
    10801 cggcgaggag atcgtggcgc aggtgctgcg cgagaacgag tcgcgctggg tggggctcgg
    10861 cgaggaggac cgcgcgcgcg tggagctgat ggcgcgggcg gtggtgagca ggctgctgca
    10921 cgagccgacg ctgcggctga aggccgccga gggcgacgag tcgtacgtgt tcgtccaggc
    10981 gctgcgcgag ctgttcgcga tcgacgccgg cgagctcgag gcgcccccga gcgcggccga
    11041 ggtcacctcg ctcgacgagc gccgccggcg ccagtcgcgc tgacggtccg gctcggcacg
    11101 cgcggcagcg cgctcgcgct cgcgcaggcg cggatggtgg ccgggtggct cgacgcggac
    11161 gtggagctga tcgagatcac gacgtcgggc gacgagcgga cggcgggcgg agcccccggg
    11221 cctgcggccc gcaccgggcg ccccgggcct ccggcccgtg ccgggcgccc cgggcctccg
    11281 gcccgtgccg ggcgcgtggc ttcgcccccc accgacaagt cgcgcttcgt caaggagatc
    11341 gaggaggcgc tgctgcgcgg cgatgtcgac ctcgcggtcc actccgcgaa ggacgtgccg
    11401 ggggagctgc ccgagggtct cgcgatcgtc ggggtgccgg agcgcgcgga cccgcgcgac
    11461 tcgctctgcg gcgccgcctc gctcgacgcg cttgccgagg gcgcgcgcgt gggtacgagc
    11521 agcctgcgcc gccgcgcgca gctgctcgcc gcgcggcccg acctgcgcgt ggaggacgtg
    11581 cgcgggaatg tggacacgcg gctgcggcgc ctcgccgacg gcgactacga cgccctggtc
    11641 ctcgcggcgg cggggctggc gcggctcggc cgcgacgagg gctccgcgat cccggccgcc
    11701 acgatggtgc ccgcgcccgg ccagggttgc ctcgcgctcg aggcacgcgc gggcgacgcc
    11761 gagatggccg tgctcgccgc ggcgctcacc gaccgcgcgg cgctgacggc gctgacggcc
    11821 gagcgcgcgc tggtgggcga gctggaggcg acgtgcaaca cgccgctggc ggcgtatgcg
    11881 gagctcgtga cgagcgccgc tcccggcgga gccgcgccgc ctccgggcga agccgctgcg
    11941 ccgggcgaag ccgctgcgcc gggcgaagcc gccgcgccgg gcgaagccgc cgcgccgggc
    12001 gaggccgctg cgccgggcgg agccggtgcc ggcacactcc gcctcaccgc cttcgccggc
    12061 ctcccggacg gcagccactg gatcaccgac gtgctcgagg gcgacgcggc cgatcccgcc
    12121 gcgctcggcc gcgccatggc cgagc
//
LOCUS       DASVPL010000043         6278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_358000, whole genome shotgun sequence.
ACCESSION   DASVPL010000043 DASVPL010000000
VERSION     DASVPL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358000"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..362)
                     /locus_tag="VF517_02480"
     CDS             complement(60..362)
                     /locus_tag="VF517_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8051828.1"
                     /translation="MEVQRSLHDLAGRFDLKDLDALEAEPRRRPGRTADLTRRERDVL
                     ERLAEGRATGEVAELLHVSPHTVRSRVKSVLRKLGARNREHAVAIALSEGAIEPEL"
     gene            446..601
                     /locus_tag="VF517_02485"
     CDS             446..601
                     /locus_tag="VF517_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051829.1"
                     /translation="MRLPVQLLVLAAAFAGGALIAGAAGAANLGTALAFGQIAFVIAL
                     VATLLRT"
     gene            598..954
                     /locus_tag="VF517_02490"
     CDS             598..954
                     /locus_tag="VF517_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEX8051830.1"
                     /translation="MTTALVAVGGLAGVLARYGIGRATAGSEALLWSTVGINLAGSFM
                     LGLLVGSSWFSRDVREGLGVGFLGGFTTFSTFSVQIVTEADAGRTGTAALYLAASVVG
                     GIAAAAAGYALARTAH"
     gene            1110..2027
                     /locus_tag="VF517_02495"
     CDS             1110..2027
                     /locus_tag="VF517_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HEX8051831.1"
                     /translation="MASDEHVAAVRGFNRFYTNVAGLLREGLLGTDLTLTEARVVFDL
                     AQREQTEVAALRAALALDAGYLSRVIGRLESRGLLERERSAQDGRRQVLTLTARGRRR
                     FQTIDARSAREVAALLDPVPESDRRRLVGAMATIEDVLGACERPASFVLRPPAPGDYG
                     WIVENHARVYSDEFGFDVRFEALIARIVGEWAADHDPHREACWIAEVDGEPAGSIFCM
                     RKSERVAKLRLLCVAPRARGTGVGSRLVDECLRFARSAGYRRIVLWTQDNLTGARRIY
                     ERAGFRLDDEEAHTMFGPEVRGQNWSRTL"
     gene            complement(2101..2661)
                     /locus_tag="VF517_02500"
     CDS             complement(2101..2661)
                     /locus_tag="VF517_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX8051832.1"
                     /translation="MIGAILAGGRGRRMGGGKPGRELAGRPLIAWPAAALAQACERVV
                     VVAKAVVELPALPGVERWDEPDDGHHPALGIAHALERAGGPVLVCAADMPFVTAEACA
                     RVAEALGDGRAAVAVAGGVIQPVFGAYAPDAAAALRAAPPDAALTRTVDALGPVLVEV
                     PAAVVRSVDTEEDLARAEAELGLRGA"
     gene            2683..3660
                     /locus_tag="VF517_02505"
     CDS             2683..3660
                     /locus_tag="VF517_02505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8051833.1"
                     /translation="MALGTFISVGRSLDSSVRRASRAEELGYDSVYTTHIAGLDSLTV
                     LAAYACATERVRLGTGVMPLYSRTPVATAQQALTIDQLSEGRTVLGIGVSHKITVENW
                     FGTEIGKPVADMREYVATLRAIFAGEEPPQDHERFRTQFRFMGPQPRADLPIYLAALS
                     PGMLRLAGEVGDGVVLWLCNPEYIREVVIPELRTGRERAGKDLDGFDVVAAVPSAVTD
                     DPDAARERLRGELITYFSLPFYRKMLERSGFGDDIERFDAKMSGGDAKGATAEISDRF
                     LNLLGAIGSADEAKASVQRYVDAGATSPCVGGVSGTDFDATLEALAPTQ"
     gene            complement(3661..4122)
                     /locus_tag="VF517_02510"
     CDS             complement(3661..4122)
                     /locus_tag="VF517_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051834.1"
                     /translation="MGDRLRIGRVLREPLFLAAVAGVVVIVAVAVIRDGDDGPPAVSG
                     TPKEAVETVEAFRRALAARDFATICDQLYTTEAREAAGGDDCQSVLAQETAKLREPRV
                     QIVGLTVRGETADVTVQASVRGQKPVQDTIRLAREKGRFRIASRGPAAGER"
     gene            4172..4780
                     /locus_tag="VF517_02515"
     CDS             4172..4780
                     /locus_tag="VF517_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051835.1"
                     /translation="MEGSAADSLGTSASWLRCYRCWSQNLEVQVHYEGVLKVDPQSGE
                     AVEPVEEIQEAVVQCLDCLHDQPHLSLAFYEENGRRISRIEAVEDRWERMISGTPWVA
                     SCTVTVDSDAVESCSGPEAADSLAFGAFGDHGTREFFTHVRFHKHDGDRIVVHMLVEL
                     YARSSEEANEVLEEAARGPLSITSLAEESRPPLATPDDEDAH"
     gene            complement(4800..>6278)
                     /locus_tag="VF517_02520"
     CDS             complement(4800..>6278)
                     /locus_tag="VF517_02520"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX8051836.1"
                     /translation="KELAANMPNARRTKYDNLNVQTEVLARSKASTFLVLDDPDSSSQ
                     QAISREEGERVARLQDEYIAGRDMVVVDGYIGNDPDFRTPARLYIEESNANIAGMQKQ
                     LYFDVEDRDGFEPELTVIYTPNLKVEGFPNDRVISVDLERGVTRVCNSDYFGESKKGG
                     LRMWNKLVFDRGGLPLHAGCKVIPTDSGDKVGLIVGLSGTGKTTTTFTRQNGSLPVQD
                     DFVAMMPGGKVYATEAGCFAKTFALSTEDEPTIHGAVTRPEAYLENVSQDESGELDFF
                     DTSYTQNGRATFSFSSIEAGDARDLQAADFLLILNRNENIIPAVAKLNAEQAAAYFML
                     GETQGTSAGGAEEAGKFLRVPGTNPFFPDDHDHQGNRFLELMAEHQLDVYLMNTGRVG
                     GPDGDERSAKVRIPHSSAIVKGVAEGTIEWETDPDFGYQVATRVPGIEDSDAGVLRPR
                     ELYESQGREDEYRRIVDTLTGERVEFLQKFPGLTSEIVDAVR"
BASE COUNT          815 a         2387 c         2134 g          942 t
ORIGIN      
        1 cgcgcagcgg ctccgcccgc gccgccgccc acgcgccgcg cggccgcccg ccgcgccgcc
       61 tacagctccg gctcgatcgc gccctcgctc agtgcgatcg ccactgcgtg ctcgcggttg
      121 cgcgcgccga gcttgcgcag cacgctcttc acacggctgc gcacggtgtg cgggctcacg
      181 tggagcagct cggccacctc cccggtcgcc cggccctccg cgagccgctc gagaacgtcg
      241 cgctcgcgac gggtgaggtc ggccgtgcgg cccggccgcc ggcgcggctc ggcctcgagc
      301 gcgtcgaggt ccttcaggtc gaaccggccc gcgaggtcgt gcaagctgcg ctgtacctcc
      361 acctctcgga aagctactgg tgacgcaggc gtcaaacacc gcctgggcgg ccttgcaacg
      421 gtccttgcgc ctatcctgcc cgcgcgtgcg gctccccgta cagctcctgg tgctcgccgc
      481 ggcgttcgcg ggcggagctc tgatcgccgg cgcggccggc gcggctaacc tcggcacagc
      541 cctcgcgttc gggcagatcg cgttcgtcat agcgctggtc gcgactctgc tgcgcacatg
      601 accaccgcgc tcgtggcggt cggcggcctc gcgggggtgc tcgcgcgcta cggcatcggc
      661 cgtgccacgg ccggcagcga ggcgctgctg tggtcaacgg tcgggatcaa cctcgccggc
      721 tcgttcatgc tcggcctgct cgtcggcagc tcgtggttct cgcgcgacgt gcgcgagggg
      781 ctcggcgtcg ggttcctggg cggcttcacg accttctcca cgttctcggt ccagatcgtc
      841 accgaggcgg acgccggccg cacgggcacc gccgcgctgt acctggcggc gtcggtggtg
      901 ggcggcatcg ccgctgcggc cgcggggtac gcactcgcac gcaccgcgca ctagggcgct
      961 gcgccataca ccgctcggcg cgcgccgcgc actaggcgcc gcgccatacg ccgctcggcg
     1021 cgcgccgccg ctcagcgccg cgcccgtacg cagctccccg cggtttcgtg cttccgcatt
     1081 agttgcctca ggcaagctat tatctgcctg tggccagtga cgagcacgtc gccgccgtgc
     1141 gcggcttcaa ccgcttctac acgaacgtcg ccggcctgct gcgcgagggg ctcctcggta
     1201 ccgacctgac cctcaccgag gcgcgggtgg tcttcgacct cgcccagcgc gagcagaccg
     1261 aggtggcggc gctgcgcgcg gccctggcgc tggacgccgg ctacctgagc cgcgtgatcg
     1321 gccggctgga gagccgcggc ctactggagc gcgagcgctc cgcgcaggac ggtcgccgcc
     1381 aggtgctcac cctcacggca cgcggccggc gtcgcttcca gacgatcgac gcgcgctcgg
     1441 cgcgcgaggt cgccgcgctg ctcgaccccg tcccggagag cgaccggcgc cggctcgtgg
     1501 gcgcgatggc gacgatcgag gacgtgctcg gcgcgtgcga gcgccccgcg tcgttcgtgc
     1561 tgcgcccgcc cgctcccggc gactacgggt ggatcgtcga gaaccacgcc cgcgtctact
     1621 ccgacgagtt cggcttcgac gtgcgcttcg aggcgctgat cgcgcggatc gtcggcgagt
     1681 gggcggccga ccacgacccc caccgcgagg cgtgctggat cgcggaggtg gacggcgagc
     1741 cggccgggag catcttctgc atgcgcaagt cggagcgggt ggcgaagctg cgtctgctgt
     1801 gcgtcgcccc gcgggcgcgc gggacgggcg tcggcagccg cctggtggac gagtgcctgc
     1861 gcttcgcccg cagcgcgggc taccgccgca tcgtcctctg gacccaggac aacctcaccg
     1921 gcgcgcgtcg gatctacgag cgcgcgggct tcaggctcga cgacgaggag gcgcacacga
     1981 tgttcgggcc cgaggtgcgc ggccagaact ggtcgcgcac gctctaggcg cccggcgtcg
     2041 agcacccgaa gtcagaaacg tccctatgtg ggacgcttct gacttcaccc ggccgcgcac
     2101 ctacgcgccg cgcaggccga gctccgcctc ggctcgcgcc aggtcctcct ccgtgtccac
     2161 gctccgcacc acggctgccg gcacctctac cagcaccggc cccagcgcgt ccacggtccg
     2221 cgtgagcgcc gcatcgggag gggccgcgcg cagcgccgcg gcggcgtccg gcgcgtaggc
     2281 cccgaacacc ggctggatca ccccgcccgc gaccgccacc gccgcccgcc catcccccag
     2341 cgcctccgcc acgcgcgcgc acgcctcggc ggtcacgaac ggcatgtccg ccgcgcacac
     2401 gagcacgggc ccgccagcgc gctcgagcgc gtgtgcgatc ccgagcgcgg ggtgatggcc
     2461 gtcgtcgggc tcgtcccagc gctcgactcc cggcagcgcg ggcagctcga ccaccgcctt
     2521 cgccaccacc acgacgcgct cgcacgcctg cgccagcgcg gccgctggcc acgcgatcag
     2581 cggccgcccc gcgagctcgc ggcccggctt tccaccgccc atccgccgcc cccggccgcc
     2641 ggcgagtatc gccccgatca tggcgcgtat gctcccagcc ccttggccct cggcacgttc
     2701 atctcggtag gacgctcact cgactcctcc gtgcgccgcg cgagccgcgc cgaggagctc
     2761 ggctacgact cggtctacac gacgcacatc gcggggctcg actcgctcac ggtcctcgcc
     2821 gcctacgcct gcgccaccga gcgcgtgcgg ctcggcaccg gcgtaatgcc gctctactcg
     2881 cgcacgccgg tcgcgaccgc gcagcaggcg ctcacgatcg accagctctc ggagggccgg
     2941 accgtgctcg gcatcggcgt ctcgcacaag atcaccgtcg agaactggtt cggcaccgag
     3001 atcggcaagc cggtggccga catgcgcgag tacgtggcca cgctgcgcgc gatcttcgcg
     3061 ggcgaggagc cgccccagga ccacgagcgc ttccgcaccc agttccgctt catgggcccg
     3121 cagccgcgcg cggacctgcc gatctacctg gccgcgctct cgcccgggat gctgcgcctg
     3181 gccggtgagg tcggcgacgg ggtcgtgctc tggctctgca acccggagta catccgcgag
     3241 gtggtcatcc ccgagctgcg caccggccgc gagcgcgccg gcaaggacct cgacgggttc
     3301 gacgtggtgg ccgcggtgcc gagcgccgtg accgacgacc ccgacgccgc tcgcgagcgc
     3361 cttcgcggcg agctgatcac ctacttctcg ctgcccttct accgcaagat gctcgagcgg
     3421 tcgggcttcg gcgacgacat cgagcgcttc gacgcgaaga tgtccggcgg cgacgccaag
     3481 ggggccacgg ccgagatctc ggaccgcttc ctgaacctgc tcggcgcgat cggatcggcc
     3541 gacgaggcga aggcctccgt gcagcgctac gtggacgcag gcgccacctc gccgtgcgtc
     3601 ggcggcgtct cgggcacgga cttcgacgcg acgctcgaag cgctcgcgcc gacccagtag
     3661 ctaccgctcg cccgcggcgg gcccccgcga ggcgatccgg aagcgcccct tctcgcgcgc
     3721 cagccggatc gtgtcctgca ccggcttctg gcccctcacg ctcgcctgca cggtcacgtc
     3781 ggcggtctcg ccgcgcacgg tcagcccgac gatctgcaca cgcggctcgc gcagcttggc
     3841 ggtctcctgc gccaggaccg actggcagtc gtcgcccccg gccgcctcgc gggcctcggt
     3901 cgtgtagagc tggtcgcaga tcgtcgcgaa atcgcgcgcc gcgagcgccc gcctgaacgc
     3961 ctcgaccgtc tcgaccgcct ccttaggcgt gccgctcacc gcggggggtc cgtcgtcccc
     4021 gtcgcggatc accgcgaccg ccacgatcac gaccacgccc gcgaccgccg cgaggaacag
     4081 aggctccctc aggacccgcc cgattcgcaa ccgatcgccc atccgggact tatagctgtt
     4141 cgggctggac gctctccgcc ataatccgcg catggaggga tcggccgcgg actcgctcgg
     4201 gacgtcggcg agctggctgc gctgctatcg ctgctggtcg cagaacctgg aggtccaggt
     4261 ccactacgag ggcgtgctta aggtcgatcc ccagtcgggg gaggccgtcg agcccgtgga
     4321 ggagatccag gaggcggtcg tccagtgcct cgactgcctg cacgaccagc cccacctgag
     4381 cctcgccttc tacgaggaga acggccgccg gatcagccgc atcgaggccg tcgaggaccg
     4441 ctgggagcgg atgatctccg gcacgccgtg ggtggcctcc tgcaccgtca cggtggactc
     4501 cgacgccgtc gagagctgct ccggccccga ggccgccgac tcgctggcgt tcggcgcctt
     4561 cggcgaccac ggcacgcgcg agttcttcac ccacgtgcgc ttccacaagc acgacggcga
     4621 ccggatcgtc gtgcacatgc tcgtcgagct ctacgcgcgc tcgtccgagg aggcgaacga
     4681 ggtgctggag gaggccgcgc gcggccccct ctcgatcacc tcactcgccg aggagtcgcg
     4741 acccccgctc gccactcctg acgacgaaga cgcgcactag cggcgcgtct ccgcccccac
     4801 tagcgaaccg cgtcgacgat ctcgctggtc aacccgggga acttctgcag gaactcgacg
     4861 cgctcgcccg tgagcgtgtc gacgatccgc cggtactcgt cctcgcgccc ctgcgactcg
     4921 tagagttcgc gcgggcgcaa cacgccggcg tccgagtcct cgatgccggg cacgcgggtg
     4981 gcgacctggt atccgaagtc cgggtcggtc tcccactcga tcgtgccctc ggcgacgccc
     5041 ttcacgatcg cgctcgagtg cgggatgcgc accttcgccg agcgctcgtc gccgtccgga
     5101 ccgcccacgc ggccggtgtt catcaggtac acgtcgagct ggtgctcggc catgagctcc
     5161 aggaagcggt tgccctggtg gtcgtggtca tcggggaaga acgggttcgt ccccggcaca
     5221 cgcaggaact tcccggcctc ctcggccccg cccgcgctcg tgccctgcgt ctcgccgagc
     5281 atgaagtacg ccgcggcctg ctcggcgttc agcttggcga ccgccgggat gatgttctcg
     5341 ttcctgttga ggatcagcag gaagtcggcg gcctggaggt cccgcgcgtc gcccgcctcg
     5401 atcgacgaga acgagaacgt cgcgcgcccg ttctgcgtgt agctcgtgtc gaagaagtcg
     5461 agctctccgc tctcgtcctg cgacacgttc tcaaggtacg cctcgggccg ggtgaccgcc
     5521 ccgtggatcg tcggctcgtc ctcggtcgag agggcgaacg tcttggcgaa gcagccggcc
     5581 tcggtcgcgt agaccttgcc gcccggcatc atcgccacga agtcgtcctg caccgggagc
     5641 gagccgttct gacgcgtgaa cgtcgtcgtg gtcttgccgg tgcccgacag gcccacgatc
     5701 aggccgacct tgtcgcccga gtccgtcggg atgaccttgc agcccgcgtg cagcggcagc
     5761 ccgccgcgat cgaacacgag cttgttccac atgcgcagcc cgcccttctt cgactcaccg
     5821 aagtagtcgg agttgcagac gcgcgtgacg cctcgctcga ggtccacgga gatcacgcga
     5881 tcgttcggga agccctcgac cttgaggttg ggcgtgtaga tgacggtgag ctccggctcg
     5941 aagccgtcgc gatcctcgac atcgaagtag agctgcttct gcatgccggc gatgttggcg
     6001 ttcgactcct cgatgtagag ccgtgcgggc gtgcggaagt ccgggtcgtt gccgatgtag
     6061 ccgtccacga cgaccatgtc acgaccggcg atgtactcgt cctgcaggcg agcgacgcgc
     6121 tccccctcct cgcgcgagat ggcctgctgc gaagaggagt cggggtcgtc tagaaccagg
     6181 aaggtggacg ccttcgagcg ggcgagcacc tccgtctgga cgttcaggtt gtcgtacttc
     6241 gtccgccggg cgttcggcat gttcgccgcg agctcctt
//
LOCUS       DASVPL010000044        12911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_318073, whole genome shotgun sequence.
ACCESSION   DASVPL010000044 DASVPL010000000
VERSION     DASVPL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12911
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318073"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VF517_02525"
     CDS             complement(<1..596)
                     /locus_tag="VF517_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051837.1"
                     /translation="MKRKLTRRVAVIAGATVLALSGGTYAVAHGIGGGEKEQQAFLDD
                     AANRLNVTPEQLTKALKEAAAARIDQAVKDGRITKAQGDEMKQRLEQGGGVPFLGGPG
                     GPGHGGPGGHHGPPPGMDAAADYLGLSEDQLRDQLMDGKSLADVAKAKNKSVDGLKDA
                     MEKAIRADIAKAVKAKRLTQQQADRILQDLDSRLDERV"
     gene            complement(633..2129)
                     /locus_tag="VF517_02530"
     CDS             complement(633..2129)
                     /locus_tag="VF517_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8051838.1"
                     /translation="MIRSPRLPSDERLATLAGSGSDPAFAMLYRRYDGALHGYSLSIV
                     RNADDARDVLQSAWMKALVALRAQSRKAPVRPWLFRIVHNEAIDLLRRRRGDDEPLVD
                     SERLRGSAGADEEVVGRERMAEVVADLHALPEGQRAALVMREWADLEYADIALALATS
                     EGNARQLVFTARSGLVESRAGREQSCDAIRMELAGADGRRLRNRRIRSHLETCAGCRE
                     FSSETRGRRRAAAAALLPWGVGAPELFGGILSAGGAAGGVTGGSALLGGLAKGAAVAA
                     LLTGGVGTAELALDREGASGAGDRAGAVEVAGGLPQRVPGDVSMTPAVAGRKAVVAAA
                     GGGGGATRRTVALAAEREAADVVLVRDGSGDGAPGRDGERRERGGEGPGGDGTREAAR
                     GRGGPGGPGGSGGSGGSGGPGDGSGSGWDGGGSRSGSGRGGGSGSAWGSSASTSGSGS
                     GWDGGSTSGSGWDGGSGSGWDGGSTERQRRTSTHTDFLRAPSGSHTDG"
     gene            complement(2237..3022)
                     /locus_tag="VF517_02535"
     CDS             complement(2237..3022)
                     /locus_tag="VF517_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051839.1"
                     /translation="MFQRKILMLAVLVASFALLAPSALARGGHHGGPGERDARGAAID
                     RQDVPSRVSSRLKRASRALDKAEERVDDGETAGAISSLSAVRKNLASAQKSGNKRVTA
                     NADDGPASAWALTNAQHKIVDATAALFDGVTETTLVSEIEETLDAALTGRDATVDAIG
                     ALPVADQQAYRHVLEGIDEEIDDEVEAIDEALSDDTLTPEATTALNDAKAQAQATQTE
                     VRALLTALGSSSTSASQVSDGQAGDGRDCPEDGSTRAGAPARS"
     gene            complement(3096..3809)
                     /locus_tag="VF517_02540"
     CDS             complement(3096..3809)
                     /locus_tag="VF517_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051840.1"
                     /translation="MTVRDKTVLVTGANRGIGQALAAEALARGARRVYAGTRQTLDHP
                     DDRVTPVSLDVTDSEQIQAAAEQVESLDMLINNAGIALFEDLSDPDALERHLAVNLFG
                     TYAVTQAFLPALTRSRGAIVNMLSVNSLAPLPFIIPAYSVSKAAAFSLTQSLRARVAE
                     QGVRVHAVLPGPVDTDMTRGADIPKASPESVALAIFDAVENGEEEIFPDPASAPMAAP
                     WRDGAVKALERQFAQMAPS"
     gene            complement(3847..4845)
                     /locus_tag="VF517_02545"
     CDS             complement(3847..4845)
                     /locus_tag="VF517_02545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX8051841.1"
                     /translation="MSIPATMRALQQTSLDGPRDLRLISDAPVPSPGPGEILIRVTAA
                     GVNFVDISQARGTFAGGPQPPYLAGIEGAGEVTAVGEGVTGLEPGMHVIGTGIKGGAF
                     AEYMVLPAVAALPVPAGWTDEQALGMVVHWPTAMAALKPLGGVTAGQTVLIHAAAGGT
                     GQAAVKIAKHYGATVIATASPDKHEVVRALGADHVIDSRGTDIAAEVVGLTDGAGVDL
                     VLESVGGATLDASLAAAKRVTGRVVVYGLAGGEAAITNWDLVYRHQVHVIGLNIGILI
                     QTAPQIFGEVMGQLFELLAAGVLGPAQPTAYELTDGPKALADLEARATVGKLALVP"
     gene            complement(4974..5960)
                     /locus_tag="VF517_02550"
     CDS             complement(4974..5960)
                     /locus_tag="VF517_02550"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8051842.1"
                     /translation="MPDRAEFVRDTEPFRRELLAHCYRMLGSIQDAEDLVQETYVRAW
                     QSYGRFEGRSSVRTWLYQIATNRCLTELGRSSRRVLPSGLSDPEPDPGAYPEEAGPEV
                     SWLEPVPDAMVTPDSADPATIVAAREGLRLALIASLQHLPPRQRAVLVLRDVLAFPAT
                     EVADMLGTTTASVKSALQRARARVEELGLAAGHVSEPTAPQARALLDQYIEAFENADA
                     SALERLLVEHATIEATPLRTWFAGRETCIPFLRDHLLGSPGDWRMLATSANGQPAAAV
                     YTRDRDGDYQPYGICVFTMTDAGILRVVSFGDPRLVTTVFGFRPDMAQATPA"
     gene            complement(5985..6617)
                     /locus_tag="VF517_02555"
     CDS             complement(5985..6617)
                     /locus_tag="VF517_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051843.1"
                     /translation="MPDATQLVTLPLRLGVRAARLVLDPLVGAAREALGLAGDESDGH
                     REEERTESRPPDPRRSAPVAPTTEASHAEPASEPEPGLRENEPSGAPDAAPPSALVEP
                     APVLTEDAAGPVPPSEYDEPVHVSEEPVLVAESADPETADGIHTDLHVAEPWDGYRSQ
                     NAAEVIAALEDSSPAQIAVVQLYEGTHRRRKTVLEAAERQLRLASGPGRS"
     gene            complement(6623..8206)
                     /locus_tag="VF517_02560"
     CDS             complement(6623..8206)
                     /locus_tag="VF517_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8051844.1"
                     /translation="MTIGRRLSLSAAVAVAVAVALASVASYVAVRSKMRGEVDNSLRD
                     RAAAVTRFAREDPGGPEGRRGGEAGGPGGADAGGGPGGADAGGGPGGAPPPGGPPPGV
                     PTRDGSAPGTPGGDDARRPPRGPLPPPPSGRFGGAEGYVQFVRADGTAYAPRGQSGTA
                     LPVDDRTRGVASGGSEEFLADRDVDGEHLRVLTAPVESGEAVQVARPLAEVDSVLRSL
                     TAIFATFTLIGVALATGLGAIVTRAALAPVRRFTERTESVARERDVGRRLPEGEDDEL
                     GRLARTFNSTLDELERSVTAQRQLVADASHELRTPLASLRTNIELLQSGREIPERDHR
                     ELMDDVREEIDELTRLVSDVVELARRGESEADRDEVALDEIVAAAVERARRRAPATIF
                     DTRLEPALVHGAPDRLDRAVTNLLDNAAKWGGDGRVEVHLTGAGELTVRDHGPGFEEA
                     DLPHVFDRFYRARAARGMRGSGLGLAIVRQTAESHGGTVGAENAPGGGALLRLRLPVA
                     DAPERARDGSGWPESQPGT"
     gene            complement(8203..8868)
                     /locus_tag="VF517_02565"
     CDS             complement(8203..8868)
                     /locus_tag="VF517_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8051845.1"
                     /translation="MLVTDDERSVRSAVRRALTLEGYRVSEAEDGAEALSSISRDRPD
                     ALVLDVMMPELDGLQVCRALRDSGDALPVLMLTARDEIDQRVRGLDAGADDYLVKPFA
                     LEELLARLRALLRRSVPDDGGVLRFEELELDLNLYEARVSGASVRLTRTEFGLLELFL
                     RHPRQVLPRSMINERVWGYDFGPGSNSLGVYVGYLRRKLSDAGARELIQTVRGVGYIL
                     REP"
     gene            complement(8941..9462)
                     /locus_tag="VF517_02570"
     CDS             complement(8941..9462)
                     /locus_tag="VF517_02570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX8051846.1"
                     /translation="MRRVLLCTALALVCLGSADASAYEIAGRAWPAGDITYHVPAGEL
                     KKPVKRAARVWNARNLGVRFVSSAEDAARVLVSRGQSNCGGAARVGYPGRRGVSVMQI
                     HPGCGSGVSTLTAVHEFGHVLGLGHEGETCARMNPTFGSGGTPGRCDRHSLSYWLDHP
                     LTKDDVRGARSLY"
     gene            complement(9459..10436)
                     /locus_tag="VF517_02575"
     CDS             complement(9459..10436)
                     /locus_tag="VF517_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8051847.1"
                     /translation="MAARARAEDALSFAAPGFLAAFALVPLAIAALVLSRRRARRYAV
                     RFPAASSLALAAGAVPAWRRQLPTVAALLAICSLVLALARPERTVAVAVERSSIVMVT
                     DHSRSMLAEDVNPDRMSAAKRAARSFLDQLPAAVRVGAVAFSDFPDAIRAPSQDHDDA
                     RGVIDGQVADGGTATGEALASALDLLRRERQGERKTPTAIVLLSDGKTTIGRNPVEVA
                     VESRRLGIPIYTVSLGTTNATVPNPGFGPPLPATPDPETLAEIADRSGGRAFSAEDEG
                     ELATVYKTLGSRLGTKQRKREVTGAFAAGGLVLLLAAAAMSIRGGARIP"
     gene            complement(10403..11314)
                     /locus_tag="VF517_02580"
     CDS             complement(10403..11314)
                     /locus_tag="VF517_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8051848.1"
                     /translation="MRRSSLLRPPAARQGPGPMPSRLVDELDTPMARRASGALPGDRR
                     AAGLGAGTELAQMRPYEPGDDVRHIDAAATARTGEPYVKLHVPERTLTTWLVVDVSPS
                     MAFGTAARLKSDVAEGVAIVMSRLAVRRAGRVALLTFGSGESRLLRPRASRPGVMAVR
                     RALEHGVAPDGRTEPDALATALNHIRKVAAQPGLVVVVSDFRDQEDWTRPLGALTMRH
                     SVVAVEVRDPRESTVPAVGRLALVDPETGQRVEVDTSRRAVRERFATIEGERRAAVAE
                     AMRRMRVEHVVLSTADDWLRELGRRMH"
     gene            complement(11311..12336)
                     /locus_tag="VF517_02585"
     CDS             complement(11311..12336)
                     /locus_tag="VF517_02585"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8051849.1"
                     /translation="MDYEHDTAPGYREDPRVALGEALSEMKRVIVGQDAMLERLMVAL
                     LAGGHVLLEGVPGLAKTLTVKTLAEVLGGSFKRIQFTPDLVPADLVGTRIYRPNTGQF
                     DVELGPVFGNFLLADEINRAPAKVQSALLEVMQEQQVTIGGTTYEVPRPFLVLATQNP
                     IEAEGTYPLPEAQVDRFLMKILVDYPTRGEEAAVVGRSLGEPAEVRELLSLEELARFS
                     HATRSVLVDREVIGYTVAIADATRRPAAYGLDPLAPLIEWGASPRGPIGLVQAARALA
                     LLRGRGHVTLTDIRDLAADVLRHRLVLSYEALSADVSADDVIDRVVSAVIGPRAEQME
                     HHMAVAG"
     gene            complement(12357..>12911)
                     /locus_tag="VF517_02590"
     CDS             complement(12357..>12911)
                     /locus_tag="VF517_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8051850.1"
                     /translation="QPRVLQSPTTDRQEINTAIDSMEPSGGTATGEAIGASLRALDRR
                     PNATGRRPPAALVLLSDGASTSGRDPIAAAQDAKRLGVRVYTVTLGTAEGTINVPRKG
                     GGTEVQQVPPDPQSLAAIARASGGKAFTAETATGLQEVYEQLGSQLSHRDEKRQVTSA
                     FAGGGLVLLLAAAALSLRWFGRLI"
BASE COUNT         1609 a         4911 c         4678 g         1713 t
ORIGIN      
        1 tcgacccgct cgtcgaggcg gctgtcgaga tcctggagga tccggtcggc ctgctgctgg
       61 gtgagccgct tggccttgac cgccttggcg atgtcggcgc ggatcgcctt ctccatcgcg
      121 tccttgaggc cgtcgacgga cttgttcttc gccttggcca cgtcggcgag cgacttgccg
      181 tccatcagct ggtcgcgcag ctggtcctcg gacaggccga gatagtcggc cgcagcgtcc
      241 atgccgggcg ggggaccgtg atggccgccg gggccaccgt ggccggggcc gccgggcccg
      301 ccgaggaacg gcacgccgcc gccctgctcg agccgctgct tcatctcgtc gccctgcgcc
      361 ttcgtgatcc ggccgtcctt gacggcctgg tcgatgcgcg cggccgcggc ctccttgagc
      421 gccttcgtga gctgctcggg ggtcacgttg aggcggttgg cggcatcgtc gaggaacgcc
      481 tgctgctcct tctcgccgcc gccgatgcca tgcgccacgg cgtaggtccc gccgctgagc
      541 gccaggaccg tcgcgccggc gatgacggcc acgcgtcgtg tcagctttct cttcatcgct
      601 gctccttcgt tcgggggtgg aaggtcatgg catcacccgt ccgtgtgaga accggaaggg
      661 gcgcgtaaga agtccgtatg agtggaggtg cgccgctggc gctcggtgga gccgccgtcc
      721 cagccggagc cgcttccgcc gtcccagccg gagccgctcg tggagccgcc gtcccagccg
      781 gagcccgagc cgctcgtcga ggcggagctg ccccaggcgg agccggagcc gccgcctcgg
      841 cccgagccgg agcggcttcc gccgccgtcc cagccggagc cggagccatc gccgggaccg
      901 cccgagccgc ccgagccgcc cgagcctccg gggccgccgg gaccgccgcg cccgcgcgcg
      961 gcttcgcgcg tgccgtcccc tcccggtccc tcgccgccgc gctcgcgccg ctcgccgtcg
     1021 cgcccgggag caccgtcgcc cgagccgtcg cgcaccagca ccacgtccgc cgcctcgcgc
     1081 tcggcggcca gcgcgaccgt gcgtcgcgtc gcgccgcctc cgccgcccgc ggcggccacg
     1141 accgccttgc gccccgccac cgcgggggtc atgctcacat cgcccgggac ccgctggggc
     1201 aggccgcccg cgacctccac cgcgcccgcg cggtcgcccg ccccggaagc gccctcgcga
     1261 tcgagcgcca gctccgccgt cccgacgccg ccggtcagga gcgccgccac cgccgctccc
     1321 ttcgcgagcc ctccgagcag cgccgagccg ccggtcacgc cgcccgcggc gccgccggcc
     1381 gagaggatcc cgccgaacag ctcgggcgct cccacgcccc acggcagcag cgcggccgct
     1441 gcggcgcggc ggcggccgcg ggtctcagac gagaactcgc ggcacccggc gcacgtctcg
     1501 aggtgcgatc ggatccgccg gttgcgcagc cgcctgccgt cggcgcccgc gagctccatc
     1561 cggatcgcgt cgcacgactg ctcgcgcccg gcccgcgact cgacgagccc cgaccgagcg
     1621 gtgaacacga gctggcgcgc gttgccctcg ctggtcgcca gcgcgagcgc gatgtcggcg
     1681 tactcgaggt ccgcccactc gcgcatgacg agcgcggcgc gctgcccctc gggaagagcg
     1741 tgcaggtcgg cgaccacctc ggccatccgc tcgcgaccca ccacctcctc gtccgcaccc
     1801 gcgctgccgc ggaggcgctc ggagtcgacc agcggctcgt cgtcgccgcg ccggcgccgg
     1861 agcaggtcga tcgcctcgtt gtggacgatc cggaagagcc acggccgcac gggcgccttg
     1921 cgcgactgcg cccgcagcgc caccagcgcc ttcatccagg cgctctggag cacgtcgcgc
     1981 gcgtcgtcgg cattgcgcac gatcgacagg gagtagccgt gcagcgcgcc gtcgtagcgg
     2041 cggtagagca tcgcgaacgc cgggtcgctg cccgatccgg cgagcgtggc gagccgctcg
     2101 tcggagggga gtcggggcga gcggatcacg gtgggcagtc tcgtctcggg ttctaaaagc
     2161 cgtttgtgag tgacgtaaga atcggcgaag aacggcggcg gacgggcgcg aaagcccgcc
     2221 cgccgccgtc gctccgtcag ctacgggcgg gtgctcccgc gcgggtcgac ccgtcctcgg
     2281 ggcagtcgcg accgtcgccg gcctgaccgt cggacacctg gctcgccgac gtgctgctcg
     2341 agccgagcgc cgtcagcagc gcccggacct ccgtctgcgt cgcctgggcc tgcgccttcg
     2401 cgtcgttcag cgcggtcgtc gcctccggcg tgagcgtgtc gtcgctcagc gcctcgtcga
     2461 tcgcctcgac ctcgtcgtcg atctcctcgt cgatgccctc gagcacgtgc ctgtacgcct
     2521 gctggtcggc caccggcagc gcaccgatcg cgtcgacggt cgcgtcacga ccggtcagcg
     2581 ccgcgtcgag ggtctcctcg atctcgctca cgagcgtcgt ctcggtcacg ccgtcgaaca
     2641 gcgcggccgt ggcgtcgacg atcttgtgct gtgcattcgt gagcgcccac gcggaggcgg
     2701 ggccgtcgtc ggcgttcgcc gtgacccgct tgttgcccga cttctgagcc gaggcgaggt
     2761 tcttgcggac cgccgacagc gagctgatcg cccccgccgt ctcaccgtcg tccacgcgct
     2821 cctcggcctt gtcgagcgcg cgactggcac gcttgagccg gctcgacacg cggctcggca
     2881 cgtcctggcg gtcgatcgcg gcgccgcgcg cgtcgcgctc gccggggccg ccgtggtgcc
     2941 cgccgcgcgc cagcgccgac ggcgccagca gggcaaagct cgcgaccaac accgcgagca
     3001 tcaggatctt gcgttggaac atctggcctc ctcgtggggc gtcgactggg gacacccgaa
     3061 ctacgagggg gcgcggcgtg cgttaggagg ttcctctacg agggagccat ctgcgcgaac
     3121 tggcgctcga gagccttgac cgcaccatcg cgccagggcg ccgccatcgg cgcggaggcg
     3181 ggatcgggga agatctcctc ctccccgttc tcgaccgcgt cgaagatggc cagcgcgacc
     3241 gattccgggg atgccttcgg tatgtcggcg ccgcgcgtca tgtcggtgtc caccgggccc
     3301 ggcaggaccg cgtgaaccct caccccctgc tcggcgacac gagcgcgaag cgactgggtc
     3361 agggagaacg cggccgcctt cgagacggag taggccggaa ttatgaacgg caacggggcg
     3421 agcgagttca ccgagagcat gttcacgatg gccccgcggg agcgggtcag cgccggcagg
     3481 aaggcctgcg tcacggcgta ggtcccgaag aggttcacgg ccaggtggcg ttcgagtgcg
     3541 tcggggtcgg acaggtcctc gaacagggcg atgccggcgt tgttgatcag catgtcgagc
     3601 gactcgacct gctcggcagc cgcctggatc tgctccgagt cggtgacgtc caggctcacg
     3661 ggcgtcaccc ggtcgtccgg atggtccagc gtctggcggg tcccggcgta cacccgcctg
     3721 gcgcccctgg ccagggcttc ggccgccagc gcctgcccga taccgcggtt ggccccggtc
     3781 accaggactg tcttgtctct cacggtcatg tctttctctc ctgtcatagg cgtgatcgag
     3841 cggaggtcag gggaccaggg ccagcttccc cacggtggcc cgggcttcca gatccgcgag
     3901 cgccttcggg ccgtcggtga gctcgtacgc ggtgggctgc gcgggcccga gcaccccggc
     3961 ggcgagcagc tcgaacagct ggcccatgac ctcaccgaag atctgcggtg cggtctggat
     4021 caggatgccg atgttcaggc cgatcacgtg gacctggtgc cggtagacca ggtcccagtt
     4081 ggtgatcgcg gcctccccgc cggccagtcc gtagacgacg acccggccgg tgacccgctt
     4141 ggccgcggcc aggctggcgt cgagggtggc gccgccgacc gactccagca ccaggtcgac
     4201 gcccgcgccg tcggtcagcc ccaccacctc ggcggcgatg tcggtgccgc gggagtcgat
     4261 cacgtggtcg gcgcccagcg cccgcaccac ctcgtgcttg tccggcgagg ccgtggcgat
     4321 caccgtcgcc ccgtagtgct tggcgatctt caccgcggcc tggccggtcc cgccggcggc
     4381 ggcgtggatc agcacggttt ggcccgcggt cacgccgccc agcggcttga gcgccgccat
     4441 cgccgtcggc cagtgcacga ccatgcccag cgcctgctcg tcggtccagc cggccggcac
     4501 cggtagcgcg gcgaccgcgg gcagcaccat gtactcggcg aaggcgccgc ccttgatgcc
     4561 ggtgccgatg acgtgcatgc cgggctccag gccggtcacc ccctcaccga ccgcggtgac
     4621 ctcgccggcg ccttcgatgc ccgccagata cggcggctgc gggccgcccg cgaacgtgcc
     4681 gcgagcctgc gagatgtcga cgaagttgac gccggcggcg gtcacgcgga tcaggatctc
     4741 gccggggccc gggctcggta ccggcgcgtc gctgatcagg cgcaggtccc gcgggccgtc
     4801 cagggaggtc tgctggaggg cgcgcatggt ggcggggatg ctcattccga tctgggtcct
     4861 tttccttggc ttgcgtgtcg aggtcgaact gccttcagcc agggagaccc gacgagaacg
     4921 gaaaaggaat cgctgaacgc ccaatccctc cggcgagcac cggagagagc cggctaagcg
     4981 ggcgttgcct gcgccatgtc cggccggaac ccgaacacgg tggtgaccag tcgcgggtcg
     5041 ccgaaggaga cgacccggag gatgcccgcg tcggtcatcg tgaacacgca gattccgtac
     5101 ggctggtagt ccccgtcccg gtcacgggtg tagaccgccg cggcgggctg cccgtttgcg
     5161 ctggtggcca gcatgcgcca gtcaccgggc gaccccagga ggtggtcgcg caggaatgga
     5221 atgcaggtct cgcggccggc gaaccaggtg cgcagaggcg tcgcctcgat cgtggcgtgc
     5281 tccaccagga gccgctccag tgcggaggcg tcggcattct cgaatgcctc gatgtactgg
     5341 tcgaggagag cgcgggcctg cggcgcggtc ggctcgctga cgtgcccggc ggccaggccc
     5401 agctcctcca cccgcgcccg cgcgcgctgc agcgcgctct tgaccgacgc cgtcgtcgtg
     5461 ccgagcatgt ccgcgacctc ggtggcggga aacgccagca cgtcccgcag gacgagcacg
     5521 gcccgctgcc tcggcggcag gtgctggagg ctggcgatga gcgccaggcg cagtccctca
     5581 cgcgccgcga cgatggtcgc cggatcggcc gagtccgggg tcaccatcgc gtccggcacc
     5641 ggctcgagcc agctcacctc cggcccggcc tcctccgggt acgccccggg atccggctcg
     5701 gggtcggaca ggccggacgg cagcactcgc cggctgctcc ggcccagctc ggtcaggcac
     5761 cggttcgtgg ctatctggta cagccaggtg cgtaccgagg accgtccctc gaaccgtccg
     5821 taggactgcc aggcccgcac gtacgtctcc tggaccaggt cctccgcatc ctggatcgag
     5881 ccgagcatgc ggtagcagtg cgcgagcagc tcacgccgga acggctcggt atcgcggacg
     5941 aattcagcgc gatcgggcac cagctcaagc tagcgcccgc gtactcagga ccggccgggc
     6001 ccgctcgcca ggcgaagctg gcgttcggcg gcctcgagca ctgtcttgcg gcgccggtgc
     6061 gtgccctcgt agagctgcac gacggcgatc tgcgccggcg acgagtcctc gagcgcggcg
     6121 atgacctcgg cggcgttctg gctgcggtag ccgtcccacg gctccgccac gtggaggtcg
     6181 gtgtggatgc cgtccgcggt ctccgggtcg gccgactcgg ccacgagcac cggctcctcc
     6241 gagacgtgaa ccggctcgtc gtactcgctc ggcgggaccg ggcccgcggc gtcctcggtg
     6301 aggacgggcg ccggctcgac caacgccgag ggcggggccg cgtccggcgc gccgctgggc
     6361 tcgttctcgc gcaggccggg ctccggctcg ctcgcgggct cggcgtggga cgcctccgtg
     6421 gtcggcgcga ccggcgcgga tcggcgcggg tcgggggggc gcgactcggt ccgctcctcc
     6481 tcccggtggc cgtcactctc gtcgcccgcg aggccgagcg cctcccgggc ggcaccgacc
     6541 aggggatcga gcacgaggcg agccgcgcgc acgccgaggc ggagcggcag ggtcacgagc
     6601 tgcgtggcgt cgggcacggc cgctacgtac ccggctgcga ctccggccaa ccagagccgt
     6661 cgcgtgcgcg ctcgggtgcg tcagcgacgg gcagccgcag ccgcagcagc gcgcccccgc
     6721 ccggggcgtt ctccgcgccc accgtgccgc cgtgcgactc ggccgtctgg cgcacgatcg
     6781 ccagccccag acccgagccg cgcatcccgc gggcggcgcg ggcgcggtag aagcggtcga
     6841 acacgtgcgg caggtcggcc tcctcgaagc cggggccgtg gtcgcggacg gtgagctcgc
     6901 ccgcgccggt caggtggacc tccacccggc cgtccccgcc ccacttcgcc gcgttgtcga
     6961 gcaggttggt caccgcgcgg tcgagccggt ccggcgcgcc gtgcaccagc gcgggctcga
     7021 gccgcgtgtc gaagatcgtg gccggcgccc gccggcgcgc gcgctccacg gcggcggcca
     7081 cgatctcgtc gagcgccacc tcgtcgcgat cggcctcgga ctcgcccctg cgcgccagct
     7141 cgaccacgtc cgacacgagc cgggtgagct cgtcgatctc ctcgcgcacg tcgtccatca
     7201 gctcgcggtg gtcgcgctcg gggatctcgc gcccgctctg gaggagctcg atgttcgtgc
     7261 gcaggctcgc gagcggcgtg cgcagctcgt gcgaggcgtc ggccacgagc tggcgctgcg
     7321 cggtgaccga gcgctccagc tcgtcgagag tcgagttgaa agtgcgcgcg aggcggccga
     7381 gctcgtcgtc ctcgccctcg ggcaggcgcc gccccacgtc gcgctcgcgc gcgaccgact
     7441 cggtgcgctc ggtgaagcgc cgcacgggcg cgagcgccgc gcgcgtcacg atcgcgccca
     7501 ggccggtcgc gagggcgacg ccgatcagcg tgaaggtggc gaagatcgcg gtgagagagc
     7561 gcagcacgct gtcgacctcc gcgagcgggc gggccacctg cacggcctcg ccgctctcca
     7621 ccggggccgt cagcacgcgc aggtgctcgc cgtcgacgtc ccggtccgcg aggaactcct
     7681 cggagccgcc gctcgcgacc ccgcgcgtac ggtcgtccac cggcagcgcc gtgccgctct
     7741 ggccgcgcgg cgcgtaggcg gtgccgtcgg cccgtacgaa ctggacgtag ccctcggcgc
     7801 cgccgaagcg cccggacggg ggcggcggca gaggcccgcg cggaggcctc cgggcgtcgt
     7861 cccctccggg ggtgcccggc gccgatccgt cgcgagtcgg gaccccggga ggcgggccgc
     7921 ccggaggcgg ggcgccgccg ggcccgccgc ccgcatcggc gccgccgggc ccgccgcccg
     7981 catcggcccc gccgggccct ccggcctcgc cgccgcgccg cccctccggc ccaccggggt
     8041 cctcccgcgc gaaccgcgtc accgcggccg cgcggtcgcg cagcgagttg tccacctccc
     8101 cgcgcatctt ggagcggacg gccacgtacg aggcgaccga ggcgagcgcc accgcgacgg
     8161 ccaccgccac ggcggccgac agcgacaggc ggcgaccgat cgtcacggct cgcgcaggat
     8221 gtacccgacg ccgcgcaccg tctggatcag ctcgcgcgca cccgcgtccg agagcttgcg
     8281 ccgcaggtac cccacgtata cgccgagcga gttcgagccc ggcccgaagt cgtagcccca
     8341 cacgcgctcg ttgatcatcg agcgcggaag cacctggcgc gggtggcgca ggaatagctc
     8401 gagcagcccg aactcggtcc gcgtgagcct cacggaggcg cccgacacgc gcgcctcgta
     8461 caggttcagg tcgagctcca gctcctcgaa gcgcagcacg ccgccgtcgt cgggcacgct
     8521 gcggcgcagc agggcgcgca gccgcgcgag cagctcctcg agcgcgaacg gcttgacgag
     8581 gtagtcgtcg gcaccggcgt cgagcccgcg cacgcgctgg tcgatctcgt cgcgcgccgt
     8641 gagcatcagc acgggaagcg cgtcgcccga gtcgcgcagc gctcggcaca cctggagccc
     8701 gtcgagctcg ggcatcatca cgtcgagcac gagcgcgtcg ggccgatcgc gtgagatcga
     8761 gctgagcgcc tccgcgccgt cctcggcctc gctcacgcgg tagccctcga gcgtcagcgc
     8821 ccgccgcacc gccgagcgga ccgagcgttc gtcgtctgtg acgagcacgt gcatgccatc
     8881 agtctggccg ctccggcgaa gaggctcgta agaaagcccg cgcgcggcgg gcggagcccg
     8941 ctagtagagc gaccgcgccc cgcgcacgtc gtccttcgtg agcgggtggt cgagccagta
     9001 cgacagcgag tgccggtcgc aacgtcccgg ggtgccgccc gacccgaagg tcgggttcat
     9061 gcgcgcgcag gtctcgccct cgtggcccag cccgagcacg tgtccgaact cgtggaccgc
     9121 cgtgagcgtc gacaccccgc tcccgcagcc cgggtggatc tgcatcaccg agacgccgcg
     9181 cctgccgggg tatcccaccc gcgccgcgcc gccgcagttg ctctgcccgc gtgagacgag
     9241 cacccgcgcc gcgtcctcgg cggagctcac gaaccgcacg cccaggttgc gggcgttcca
     9301 cacccgcgcc gcgcgcttga ccggcttctt cagctcaccg gcgggcacgt ggtaggtgat
     9361 gtcgccggcg ggccaggcgc ggccggcgat ctcatacgca ctcgcgtcgg cggagccgag
     9421 acagacgagc gcgagcgccg tgcagaggag gacgcgcctc acggaatccg cgctcccccg
     9481 cggatcgaca tcgccgcggc ggccagcagc agcacgagtc cgccggccgc gaacgccccg
     9541 gtgacctcgc gcttgcgctg cttggtgccg agccgcgagc cgagcgtctt gtagaccgtc
     9601 gccagctcgc cctcgtcctc ggccgagaac gcccggccgc ccgagcgatc ggctatctcg
     9661 gcgagcgtct ccggatcggg agtggcggga agcggcggcc cgaagccggg gttcggcacg
     9721 gtcgcgttgg tcgttccgag gctgaccgtg tagatcggga tgccgagccg cctggactcg
     9781 acggccacct ccaccgggtt gcgcccgatc gtggtcttgc cgtcggagag cagcacgatc
     9841 gccgtgggcg tcttgcgctc gccctggcgc tcgcggcgta acaggtcgag cgctgacgcc
     9901 agggcctcgc ccgtggcggt gccgccgtcg gccacctggc cgtcgatcac gccgcgcgcg
     9961 tcgtcgtggt cctgactcgg cgccctgatc gcgtcgggga aatcggagaa ggcgaccgca
    10021 cccacgcgca ccgccgcggg gagctggtcg aggaagctgc gcgcggcgcg cttggcggcc
    10081 gacatccggt ccgggttcac gtcctcggcg agcatcgagc gcgagtggtc ggtgaccatc
    10141 acgatcgacg agcgctcgac cgccaccgcc acggtgcgct ccggccgcgc gagcgcgagc
    10201 acgagcgagc agatcgcgag cagcgccgcg accgtgggga gctgccggcg ccaggcgggc
    10261 accgcaccgg ccgcgagcgc gagcgacgag gccgcgggga agcgcaccgc gtaacgccgc
    10321 gcgcggcgtc ggctcagcac gagcgcggcg atcgcgagcg gcaccagcgc gaacgccgcg
    10381 aggaatccgg gggccgcgaa gctcagtgca tcctccgccc gagctcgcgc agccagtcgt
    10441 cggccgtcga gagcacgacg tgctcgacgc gcatgcggcg catcgcctcc gccaccgccg
    10501 cgcggcgctc gccctcgatc gtcgcgaagc gctcacgcac cgcccgccgc gaggtgtcga
    10561 cctcgactcg ctggcccgtc tccgggtcca ccagcgcaag gcgtccgacg gcggggaccg
    10621 tcgactcgcg cgggtcgcgc acctcgacgg ccaccacgga gtggcgcatc gtcagggcgc
    10681 ccagcgggcg ggtccagtcc tcctggtcgc ggaagtccga cacgaccacc acgagccccg
    10741 gctgcgccgc caccttgcgg atgtggttga gcgccgtcgc cagcgcgtcg ggctccgtgc
    10801 ggccgtccgg cgccacgccg tgctcaaggg cgcggcgcac cgccatcact ccgggccgcg
    10861 acgcgcgcgg acgcaggagg cgcgactcgc ccgagccgaa ggtcagcagc gccacgcgcc
    10921 cggcgcgccg gacggccagc cgcgacatca cgatcgccac gccctcggcc acgtcggact
    10981 tcagccgcgc cgcggtgccg aacgccatcg acggcgacac gtccaccacg agccaggtcg
    11041 tcagcgtccg ctcgggcacg tggagcttca cgtacggctc gccggtgcgg gcggtcgcgg
    11101 ccgcgtcgat gtggcgcacg tcgtcgcccg gctcgtacgg acgcatctgc gccagctcgg
    11161 tcccggcgcc gagcccggcc gcccggcggt cgcccggcag cgcgccggag gcgcggcgcg
    11221 ccatcggcgt gtcgagctcg tccacgagcc gcgacggcat cgggccgggg ccctgccgcg
    11281 cggccggcgg ccgcagtagc gacgaccgtc tcacccggcc accgccatgt ggtgctccat
    11341 ctgctccgcg cgcgggccga tcacggcgct caccacgcgg tcgatcacgt cgtcggcgct
    11401 cacgtcggcg ctgagcgcct cgtacgagag caccagccgg tggcgcagca cgtcggccgc
    11461 gaggtcgcgg atgtcggtga gcgtcacgtg cccgcgcccg cgcagcagcg cgagcgcgcg
    11521 cgcggcctgc acgagcccga tcggcccgcg cgggctggcg ccccactcga tcagcggcgc
    11581 gagcgggtcg agcccgtacg ccgccggccg ccgcgtggcg tcggcgatcg cgaccgtgta
    11641 gccgatcacc tcgcgatcca ccagcaccga ccgcgtcgcg tgcgagaagc gcgcgagctc
    11701 ctcgagcgac agcagctcgc gcacctcggc gggctcgccg agcgagcggc cgaccacggc
    11761 ggcctcctcg ccgcgcgtgg ggtagtccac gaggatcttc atcaggaacc ggtccacctg
    11821 cgcctcgggc agcgggtagg tcccctcggc ctcgatcggg ttctgcgtgg ccagcacgag
    11881 gaacggccgc ggcacctcgt aggtcgtgcc gccgatcgtg acctgctgct cctgcatcac
    11941 ctcgagcagc gccgactgca ccttcgcggg tgcgcggttg atctcgtcgg cgagcaggaa
    12001 gttgccgaac accggcccga gctccacgtc gaactggccc gtgttcggcc ggtagatccg
    12061 cgtgcccacg aggtcggccg gcacgaggtc cggcgtgaac tggatccgct tgaacgagcc
    12121 gccgagcacc tcggcgagcg tcttcaccgt gagcgtcttc gccaggccgg gcacgccctc
    12181 gagcagcacg tgaccgcccg cgagcagcgc caccatcagc cgctcgagca tcgcgtcctg
    12241 accgacgatc acccgcttca tctcgctgag cgcctcgccc agcgccacgc gcgggtcctc
    12301 gcgatacccg ggcgccgtgt cgtgttcgta gtccatgtcg gtctcctcgt gtttggtcag
    12361 atcagtcggc cgaaccagcg cagcgacagc gccgcggcgg ccaggagcag gaccagccct
    12421 ccgcccgcga aggcggaggt gacctgccgc ttctcgtccc ggtgggacag ctgcgagccg
    12481 agttgctcgt agacctcctg gaggcccgtc gcggtctcgg cggtgaaggc cttgccgccg
    12541 ctcgcgcgtg cgatcgcggc gagcgactgc gggtccggcg gcacctgctg gacctcggtg
    12601 ccgccgccct tgcggggcac gttgatcgtc ccctcggcgg tcccgagcgt gacggtgtag
    12661 acgcgcacgc ccagccgctt cgcgtcctgc gcggcggcga tggggtcgcg gccgctggtg
    12721 gaggcgccgt ccgagagcag cacgagcgcg gcgggcgggc gccgcccggt cgcgttcggg
    12781 cgccggtcga gcgcgcgcag tgacgcgccg atcgcctcgc ccgtcgcggt gccgccgctc
    12841 ggctccatcg agtcgatcgc ggtgttgatc tcctggcggt cggtcgtggg cgactggagc
    12901 acgcgcggct g
//
LOCUS       DASVPL010000045         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_44906, whole genome shotgun sequence.
ACCESSION   DASVPL010000045 DASVPL010000000
VERSION     DASVPL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44906"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VF517_02595"
     CDS             complement(<1..818)
                     /locus_tag="VF517_02595"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8051851.1"
                     /translation="MSETETHVIVGAGLAGATAAEALREHGFAGRIVLVGADPELPYA
                     RPPLSKDYLRGESPREKARVHSDGWYAEHDVDLRIATTAERIDTHARAVTLSSGESVA
                     YDRLLIATGAEPRRLSVPGAELDGLHYLRDLADADAIAGRLEHGGRLVVIGAGWIGAE
                     VAASARMKGLDVTVVERSDVPLEHVLGHDVGALYAHIHREQGVELLTGVELEAFEGAH
                     GRVERVRLAGGRALDTDFVVVGVGVVPRTALAEHAGIAVDDGIVVDARLETSVSG"
     gene            967..1428
                     /locus_tag="VF517_02600"
     CDS             967..1428
                     /locus_tag="VF517_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051852.1"
                     /translation="MSSAVAAVLRPIPIPKTDVLRRDPAAQAFTLLRIAFTVAPILFG
                     LDKFAEVMIDDWPKYLATEFNDIIPGSAQDAMYIVGAVEIAAGLTVLVSPRFGGLLVA
                     GWLAGIIVSLLLVGGYGDIALRDFGLLLGALTLSRLASAFSDGGGAEVQRR"
     gene            complement(1491..1922)
                     /locus_tag="VF517_02605"
     CDS             complement(1491..1922)
                     /locus_tag="VF517_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8051853.1"
                     /translation="MPTRRKRTSPRSELFELIGVLARRRHQAAERSYAALGLNHTEAR
                     LLTMLAEEAGATTQDALSRRIVVDRSNAGRAFKRLEQAGYVSRRKDDADSRTNLVAIT
                     AKGRRAAAEVDELRREMARTFFRDLTDADAAAALALLRKAL"
     gene            1944..3389
                     /locus_tag="VF517_02610"
     CDS             1944..3389
                     /locus_tag="VF517_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8051854.1"
                     /translation="MRTHLSHPTIADRAEATEERGARTGRGALAGLSLAVLLASLGTS
                     IANVALPTLTQAFGASFQGAQWVVLAYLLASTTVIVSVGRLGDITGRRRLLVVGIAVF
                     TVASALCAAAPTLPALIAARAAQGLGAAIMLALAMALVSEAVPKARTGSAMGLLGTMS
                     AIGTALGPSLGGLLIGALGWRSIFLVMVPASAVAGLLAHRHVPADRRVPKRARAAFDN
                     RGTVLLALTLASYTLAMTVGRGRFGALNAALLTAAAVGVALFALAQARTRSPLIRPAL
                     LRSTRLSAGLATSALVSTVMMTTLVVGPFYLTGALGLGAAAVGLVMTIGPVVAALTGV
                     PGGRIADRFGARRTTVAGLTGIAAGSLALAAIPTTLDVAGYIAPIVVITASYAIFQTA
                     NNTSVMTGAGSDQRGVTSGMLNLSRNLGLITGASVMGAVFSLAAGTSAIETAAAGAVA
                     TAMRVTFLVAAALILAALAIATAQRTRVARA"
     gene            3415..>4535
                     /locus_tag="VF517_02615"
     CDS             3415..>4535
                     /locus_tag="VF517_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8051855.1"
                     /translation="MTSPVLWTPTAERIERAAITGFARRRGLPEDYAALHRWSVEHLE
                     EFWAAIWDEYGVEGSYDAVLASREMPGAEWFPGARVNYAGHIFKGRDGDALAIQHASE
                     SRPLQAWTWGELREQTARIRAGLVALGVGEGDRVAAFLPNIPETIAAFLATASLGAIW
                     SSAAPEFGARSVIDRFAQIEPKVLLAVDGYRYGGRDFSTAEKLEAISAEMPGTDVVRL
                     GYLDESGWEDGFLGDGPLEFASVPFDHPLWVLYSSGTTGLPKAIVHGHGGILLEHLKK
                     MHLHLDAQAGGRVFWFSTTGWMMWNFLVGVLLTEASIVLFDGSPAGDVLWDLAADSGM
                     TIFGGGAAYFAGAMKEGAEPAAGRDLSALHAVGSTGSPLS"
BASE COUNT          534 a         1654 c         1659 g          688 t
ORIGIN      
        1 ccggagacgc tcgtctcgag acgagcgtcg acgacgatgc cgtcgtccac ggcgattccc
       61 gcgtgctccg ccagcgcggt ccggggcacg acgcccactc cgacaaccac gaagtcggtg
      121 tcgagcgcgc ggccgccggc gagcctcacc cgctcgaccc gcccgtgcgc accctcgaag
      181 gcttcgagct cgacgcccgt gaggagctcg acgccctgct cgcggtggat gtgcgcgtac
      241 agcgcgccca cgtcgtggcc gagcacgtgc tcgagcggga cgtcgctgcg ctcgacgacg
      301 gtcacgtcga ggcccttcat gcgtgccgag gccgccacct ccgcgccgat ccacccggcg
      361 ccgatcacga ccagtcggcc gccgtgctcg agccgccccg cgatcgcgtc ggcgtccgcg
      421 agatcgcgca ggtagtggag cccgtcgagc tcggcaccgg gcacggacag ccgtcgcggc
      481 tcggcgccgg tcgcgatcag gaggcggtcg tacgcgacgc tttcgccgct cgacagcgtc
      541 accgcacgcg catgcgtgtc gatccgctcg gcggtggtgg cgatgcggag gtcgacgtcg
      601 tgctcggcgt accaaccatc ggagtgcacg cgtgccttct cgcgcgggga ctcgccgcgc
      661 aggtagtcct tcgacagcgg cgggcgcgcg tagggcagct cggggtcggc gccgacgagc
      721 acgatgcgtc cggcgaagcc gtgctcgcgc agggcctcgg ccgcggtggc gccggccagg
      781 ccggccccga cgatcacgtg ggtctcggtc tcggacatgg ggtggtcctc tctgcgtccg
      841 tcgggtgttc ggtcgtctgc ggtcacatgg ggtttgcgag cgcgaccgcg cgttccttac
      901 gcgacgtaag ttctgccgcg ctcccgacgc caacggcgca acaacgaact cccgaggagg
      961 agcgtcatga gtagcgctgt tgcggccgta ctgcggccga tcccgattcc caagacggac
     1021 gtgcttcgcc gcgacccggc cgcccaggcg ttcaccctgc tgcggatcgc gttcacggtc
     1081 gcgccgatcc tgttcggcct cgacaagttc gccgaggtga tgatcgacga ctggccgaag
     1141 tacctggcca ccgagttcaa cgacatcatc cccggcagcg cccaggacgc gatgtacatc
     1201 gtcggcgccg tggagatcgc cgccgggctc accgtgctcg tgagcccgcg cttcggcggt
     1261 cttctggtcg ccggttggct cgccggcatc atcgtcagcc tgctgctcgt cggcggttac
     1321 ggcgacatcg cgctgcgcga cttcggtctg ctgctcggcg cgctcacgct gtcacggctc
     1381 gccagcgcgt tctcggacgg cggcggggcc gaggtgcagc ggcgctgagg cgcggctgga
     1441 cctgaggggt gtggaggggc cgcccgcggg cggcccttcc gctttccggc ctacagcgcc
     1501 tttcgcagca gcgccagggc ggccgccgcg tcggcgtcgg tgaggtcgcg gaagaacgtc
     1561 cgggccatct cgcgacggag ctcgtcgacc tcggcggcgg cgcggcggcc cttggccgtg
     1621 atcgcgacga ggttggtgcg gctgtcggcg tcgtccttgc gccggctcac gtagccggcc
     1681 tgctcgaggc gcttgaaggc ccggccggcg ttgctgcggt ccacgacgat ccggcgcgag
     1741 agggcgtcct gcgtggtcgc gccggcctcc tcggcgagca tggtcagcag ccgggcctcg
     1801 gtgtggttga gccccagcgc ggcgtagctg cgctcggccg cctggtggcg gcggcgggcg
     1861 agcacgccga tcagctcgaa cagctcgctg cgggggctgg tcctcttgcg tcgggttggc
     1921 atgcgcgcta gtgtaataca tgcgtgcgca cgcatctatc tcatccgacc atagccgacc
     1981 gggccgaagc cacggaggag agaggcgctc gcacgggtcg cggcgcgctc gccggcctct
     2041 cgctggcggt cctgctggcc tcgctcggca cgagcatcgc gaacgtcgcg ctgcccacgc
     2101 tgacgcaggc gttcggcgcg tcgttccagg gcgcgcagtg ggtggtcctc gcctacctgc
     2161 tggcgagcac cacggtgatc gtcagcgtcg ggcggctcgg cgacatcacg ggccgccgcc
     2221 ggctgctggt cgtgggcatc gccgtgttca cggtggcctc ggcgctctgt gccgccgcgc
     2281 ccacgctccc ggcgctgatc gctgcgcgcg ccgctcaggg tctcggcgcg gcgatcatgc
     2341 tggcgctcgc catggcgctg gtgagcgagg ccgtcccgaa ggcgaggacc gggagcgcga
     2401 tgggcctgct cggcacgatg tcggccatcg gcaccgcgct ggggccgtcc ctgggcggcc
     2461 tgctgatcgg cgcactcggc tggcggtcga tcttcctcgt catggtcccc gcgagcgccg
     2521 tggcgggcct gctggcccat cgccacgtcc ccgccgaccg gcgcgtgccc aagcgcgcac
     2581 gggcggcgtt cgacaatcgc ggcacggtcc tgctcgccct gaccctcgcg tcctacacgc
     2641 tggcgatgac cgtcggccgc gggcgcttcg gcgcgctcaa cgccgccctg ctcaccgcgg
     2701 ccgccgtcgg ggtggccctg ttcgcgctgg cccaggccag gacccgctcg ccgctgatcc
     2761 gccccgcgct gcttcgctcc actcgactga gcgccggcct cgccacgagc gcgctggtct
     2821 ccacggtcat gatgaccacg ctcgtcgtcg gcccgttcta cctgacgggc gcgctcggcc
     2881 tcggcgccgc ggcggtcggg ctcgtgatga cgatcgggcc cgtggtcgcc gcgctgaccg
     2941 gcgtcccggg cggccggatc gccgaccgct tcggcgcgcg gcgcacgacc gtcgccggtc
     3001 tgaccggaat cgccgcgggc tccctggccc tcgccgcgat accgaccacg ctcgacgtcg
     3061 ccggctacat cgccccgatc gtcgtgatca ccgccagcta cgcgatcttc cagaccgcga
     3121 acaacaccag cgtcatgacc ggcgccggct ccgaccagcg cggcgtgacc tccggcatgc
     3181 tcaacctctc gcgcaacctg ggactgatca ccggcgcgtc ggtgatgggc gccgtgttct
     3241 cgctggcggc gggcacgagc gcgatcgaga cggcggcggc cggcgcggtc gccaccgcga
     3301 tgcgcgtcac gttcctggtg gccgcggccc tgatcctcgc cgccctcgcc atcgcgaccg
     3361 cgcaacgcac ccgcgtcgct cgggcctgac cggccgggtt accatcgccg cggcatgacg
     3421 agccccgtgt tgtggacgcc gacggccgag cggatcgagc gcgcggcgat caccggcttc
     3481 gcccggcgcc gcgggctgcc cgaggactac gcggcgctgc accgctggtc ggtcgagcac
     3541 ctggaggagt tctgggcggc gatctgggac gagtacggcg tggagggctc ctacgacgcc
     3601 gtgctcgctt cgcgcgagat gccgggcgcg gagtggttcc cgggcgcgcg ggtcaactac
     3661 gccgggcaca tcttcaaggg ccgggacggc gacgcgctgg cgatccagca cgcgagcgag
     3721 tcgcggccgc tccaggcgtg gacgtggggt gagctgcgcg agcagaccgc tcggatccgc
     3781 gcggggctgg tggccctcgg cgtgggggag ggcgatcgcg tcgcggcgtt cctgccgaac
     3841 atccccgaga cgatcgcggc gttcctggcc acggcgtcgc tgggcgcgat ctggtcgagc
     3901 gcagcgccgg agttcggggc gcgcagcgtg atcgaccgct tcgcgcagat cgagcccaag
     3961 gtgctgctgg cggtcgacgg ctatcgctac ggcggcaggg acttctcgac cgccgagaag
     4021 ctcgaggcga tctcagccga gatgccgggc accgacgtag tgcgcctcgg ctacctcgac
     4081 gagtccggct gggaggacgg cttcctgggc gacggcccgc tcgagttcgc gtccgtgccg
     4141 ttcgaccacc cgctctgggt gctctacagc tcgggcacga ccgggctgcc caaggcgatc
     4201 gtccacggcc acggcgggat cctgctcgag cacctgaaga agatgcacct gcacctcgac
     4261 gcgcaggcgg gcggccgcgt gttctggttc tccaccaccg gctggatgat gtggaacttc
     4321 ctcgtcggcg tgctgctcac cgaggcgtcg atcgtcctgt tcgacggcag cccggccggg
     4381 gacgtcctgt gggacctggc ggccgattcc ggcatgacga tcttcggcgg cggcgcggcg
     4441 tacttcgcgg gcgcgatgaa ggagggcgcg gagcccgccg ccggacgcga cctctcggcg
     4501 ctgcacgcgg tgggctcgac cggctcgccg ctctc
//
LOCUS       DASVPL010000046         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_358463, whole genome shotgun sequence.
ACCESSION   DASVPL010000046 DASVPL010000000
VERSION     DASVPL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358463"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VF517_02620"
     CDS             complement(<1..358)
                     /locus_tag="VF517_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HEX8051856.1"
                     /translation="MLGGAFNPPHIGHLALAQEAFARLELDEVLLMPMARAPHREIEP
                     EPGAEVRLRMTELAVQGDVRLSACPLEVDRGGRSYTVETLRALRDRSPADARFLILGG
                     DAAAGLPAWGEPEEVLR"
     gene            complement(376..1647)
                     /locus_tag="VF517_02625"
     CDS             complement(376..1647)
                     /locus_tag="VF517_02625"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX8051857.1"
                     /translation="MATTTQSVAAICAEAKQASRALASADSGTRDAALDLMAAALEMR
                     VDEILEANERDMAAGAEAGLSSALLDRLKLTPGRIDAMAGDVRTIVALPDPVGETIEG
                     HRLPNGLDVRKVRVPLGVVAVVYESRPNVTIDCSALCLKSGNAIVLRGSSMAAHSNAV
                     LAGIARDAAVAAGLPAAVISLVAGGGREELPELARQEEHLDLIIPRGGEGLKAVLKEH
                     AKVPVIYAASGNCHVFVDASADVDDAVAIAVNSKVQRPGVCNAAETLLVHSSIAPEFL
                     PRALGELRESGVELRVDARARSLAGELASSLNDATEEDWATEYLALVLAVRVVDSVDE
                     AIDHVTRYGSGHSEAIVTASTESARAFTTGVDAACVYVNASTRFTDGGQFGMGAEIGN
                     STQKLHARGPIGIRELCTFKYVAEGSGQVRS"
BASE COUNT          236 a          675 c          573 g          225 t
ORIGIN      
        1 ggcgcagcac ctcctccggc tcgccccagg ccggcagccc agcggccgca tcgccgccca
       61 ggatcagaaa ccgcgcgtcg gccggggagc ggtcgcgcag cgcccgcagg gtctcgaccg
      121 tgtacgagcg cccgccgcga tccacctcga gcgggcacgc cgacaggcgc acgtcgccct
      181 gcacggccag ctcggtcatc cgcaggcgca cctccgcgcc cggctcgggt tcgatctcgc
      241 gatgcggcgc ccgcgccatc ggcatcagga gcacctcgtc cagctcgagg cgcgcgaacg
      301 cctcctgcgc cagcgccaga tgtccgatgt gcggcgggtt gaacgccccg ccgagcatgc
      361 ccacccgctg agcggctacg acctcacctg cccggagccc tcggcgacgt acttgaacgt
      421 gcacagctcg cgaatcccga tcggcccgcg cgcgtggagc ttctgcgtcg agttaccgat
      481 ctcggcaccc atgccgaact gaccgccgtc ggtgaagcgc gtcgacgcgt tcacgtacac
      541 gcacgcggcg tcgaccccgg tcgtgaacgc ccgcgccgac tcggtgctgg cggtgacgat
      601 cgcctcggag tggccgctgc cgtagcgggt gacgtggtcg atcgcctcgt ccaccgagtc
      661 gaccacccgc accgccagga ccagcgccag gtactccgtc gcccagtcct cctcggtggc
      721 gtcgttcagc gacgaggcca gctcgccggc gagcgaacgc gcccgcgcgt cgacccgcag
      781 ctcgactccg gactcgcgca gctcgcccag cgcccgcggg aggaactcgg gagcgatcga
      841 ggagtgcacg agcagggtct ccgccgcgtt gcagacgccc ggccgctgca ccttcgagtt
      901 gaccgcgatc gcgaccgcgt cgtccacgtc ggccgacgcg tccacgaaga cgtggcagtt
      961 ccccgacgcg gcatagatga ccggcacctt cgcgtgctcc ttgagcacgg ccttcagccc
     1021 ctccccgccg cgcgggatga tcaggtcgag gtgctcctcc tggcgcgcga gctcgggcag
     1081 ctcctcgcgc ccacctccgg ccaccagcga tatcaccgcg gccggcagcc ccgcggccac
     1141 cgccgcgtcg cgcgcgatcc cggcgagcac cgcgttcgag tgcgccgcca tcgaggagcc
     1201 gcgcagcacg atcgcgttgc cggacttcag gcacagcgcc gagcagtcga tcgtcacgtt
     1261 cggccgcgac tcgtacacga ccgccaccac gccgagcggg acgcgcacct tgcgcacgtc
     1321 gagcccgttc ggcagccggt ggccctcgat ggtctcgccg accgggtcgg gtagcgccac
     1381 gatcgtgcgg acgtcgcccg ccatcgcgtc gatccgcccg ggggtgagct tcaggcggtc
     1441 cagcagcgcg gagctgagcc cggcctcggc gccggccgcc atgtcgcgct cgttggcctc
     1501 gaggatctcg tcgacccgca tctcgagcgc ggccgccatc aggtcgagcg cggcgtcgcg
     1561 cgtacccgag tcggcactcg ccagcgcgcg cgaggcctgc ttcgcctcgg cgcagatcgc
     1621 ggctacggac tgggtggtgg tggccacgat cccagggtac agccagccgc cgctcagacc
     1681 agcacgaagt tgtcccggtg gatcgcctc
//
LOCUS       DASVPL010000047         5052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_5781, whole genome shotgun sequence.
ACCESSION   DASVPL010000047 DASVPL010000000
VERSION     DASVPL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5781"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VF517_02630"
     CDS             complement(<1..398)
                     /locus_tag="VF517_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051858.1"
                     /translation="MIRNRPGLLAVPAIALVVWLVYGSGFVDYDALYALIWGDDLAGG
                     HRPPDLGARHSPTSHPLSTALGAPLSLLGDGALGALQAISILSFAALGWAAFRLGQRL
                     FGAPAGAVFALILLTRPLLVKQALSANVDIP"
     gene            complement(412..1980)
                     /locus_tag="VF517_02635"
     CDS             complement(412..1980)
                     /locus_tag="VF517_02635"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX8051859.1"
                     /translation="MARQSHPESFEEKLAQLQELREASRSGSEAAVEKQHAKGKFTAR
                     ERIEKLLDPGTFAELDTFVRHRTYDFDMQKSRPWGDAVVTGHGLIDGRPVCVFSQDFT
                     VFGGSLGEVMAEKMVKIMDLAARIGCPVIGINDSGGARIQEGVVSLGAYGDVFVRNVQ
                     CSGVIPQISLIMGPCAGGAVYSPAMTDFIFMVKETSHMFITGPDVIKTVTGEETTFEE
                     LGGAMSHNTKSGVAHFAAEDEEACLEDARYLFSFLPQNNLQTPPRVEPTDDPDRMDEA
                     LDSVVPDNPNKPYDMRDVVSLIVDDGEFFEVHEHFAKNIIVGFSRLNGRSVGIVGNQP
                     AQLAGVLDIESSEKAARFVRTCDAFNIPLVTFCDVPGFLPGTSQEWGGIIRHGAKLLY
                     AFTEATVPKLTVVTRKAYGGAYDVMNSKHMLADFNFAWPQAEVAVMGPEGAVNIIFRR
                     DIAKSPTPDDRREKLISDYKARFANPYSAAERGYIDDVIAPHETRPKLIQALEMLQTK
                     RVDRPKRKHGNIPL"
     gene            complement(2003..2350)
                     /locus_tag="VF517_02640"
     CDS             complement(2003..2350)
                     /locus_tag="VF517_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051860.1"
                     /translation="MSRTLVKSPPELWEELQGDCIQQAVGEAVVEPREPERELAWRAE
                     GAAGTARIEPSNWGTKVVLSAKVEEQVAEVGFWARLRGAKPLPSRHDDLEQKLEGVLD
                     SLGSAHRKPFARD"
     gene            complement(2409..3950)
                     /locus_tag="VF517_02645"
     CDS             complement(2409..3950)
                     /locus_tag="VF517_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8051861.1"
                     /translation="MEAVPRGYKSPYLVRARTLIAAAVCALALLVAPGSASAVTCSHP
                     ASAYAQGAGGVAAFGGPTTGDPLAPAQWGLSQIKAPAAWARGARGAGITVAVLDTGAD
                     LTHPDLQGKLLPGTDYVLKDDGCPGPQDENGHGTHVAGIVAAVTGNGTGVAGVAPDAK
                     VMPVRVLDAEGGEADTGVVYDAIRWAADNGADVINLSLADLPLSAKVDGTDEDAERAV
                     DHAWSKGAVVVAAASNESFPLCNYPAAARHAVCVAATDSAGMPSYYSNFPNSPAGALG
                     LRAPGGEGSPIFCEYSADVWSTVWPGDAVDSNPNCDLKGYETFAGSSMAAPHVSGVAA
                     LLAGRGLTNEQIVECLRTTSSNGGAYDPVYGYGSVNADAATARCAAATTPAYQPPAPS
                     AGGPSQPAPSQPPPAPGGGSAEPQVSVTVKRTTRASLAKSGRLRATVRSDGPVSVKLR
                     AVLTRGKKKSTAGTKTVKLGGAGTTKAVVSLSRSARKALTRSGSRLVLRWRAGSRTGS
                     ATATR"
     gene            4113..>5052
                     /locus_tag="VF517_02650"
     CDS             4113..>5052
                     /locus_tag="VF517_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8051862.1"
                     /translation="MRTQSNSNSLIGQVVATNVLMVVATIFAASAAANLDLGVGDQRA
                     QFGLLALSIVLVLLVNMMMLRRRFSPLDRLIERVEAIDPANPAAFEAPEGSEAVEEID
                     RLAASFRNMLGRIDAERRRSGRLVLRAQEEERRRLARDLHDEVNQALTAILLRLEALS
                     HAAPEVGDELSELKKLVNQAMAELLHLARQLRPTALDDHGLVPAIESQLRRFSAQTGI
                     KADMRTVDLPGVVQSDSEIVIYRVAQEALSNVAQHASAGHVEIGLHGRNGRGLELVVR
                     DDGRGFDPDETRDTLGLLGMAERARLVGGKLDVASTP"
BASE COUNT          676 a         1801 c         1830 g          745 t
ORIGIN      
        1 gggatgtcaa cgttcgccga aagcgcctgc ttcacgagca gcgggcgggt gaggaggatc
       61 agcgcgaaca cggcccccgc cggcgccccg aacagccgct ggccgagccg gaacgcggcc
      121 cagccgagcg cggcgaagga caggatcgag atcgcctgga gcgcgcccag cgcgccgtca
      181 ccgagcagcg acagcggcgc gccgagcgcc gtcgacagcg ggtgcgaggt gggcgagtgg
      241 cgcgcgccga ggtccggcgg ccggtgccca ccggccaggt cgtcccccca gatcagcgcg
      301 tacagcgcgt cgtagtccac gaacccgctc ccgtagacca gccagacgac gagtgcgatc
      361 gccggcacgg cgaggaggcc gggccggttg cggatcacgg cgctgctccg cttacagcgg
      421 gatgttgccg tgcttgcgct tcggccggtc gacgcgcttg gtctggagca tctccagcgc
      481 ctggatcagc ttcggccgcg tctcgtgcgg cgcgatcacg tcgtcgatgt agccgcgctc
      541 ggccgcgctg taggggttcg cgaagcgcgc cttgtagtcg gagatgagct tctcgcggcg
      601 gtcgtccgga gtcggcgact tcgcgatgtc gcggcggaag atgatgttga ccgcgccctc
      661 ggggcccatg accgcgacct ccgcctgcgg ccacgcgaag ttgaagtcgg ccagcatgtg
      721 cttcgagttc atgacgtcgt aggcgccgcc gtacgccttg cgggtgacca cggtcagctt
      781 cggcacggtc gcctcggtga acgcgtagag cagcttcgcg ccgtggcgga tgatcccgcc
      841 ccactcctgg ctcgtgccgg gcaggaagcc gggcacgtcg cagaacgtga cgagcgggat
      901 gttgaacgcg tcgcacgtgc ggacgaagcg ggccgccttc tcggaggact cgatgtcgag
      961 cacgcccgcg agctgcgccg gctggttgcc cacgatcccg accgagcggc cgttgaggcg
     1021 cgagaagccg acgatgatgt tcttcgcgaa gtgctcgtgg acctcgaaga actcgccgtc
     1081 gtccacgatc agcgacacga cgtcgcgcat gtcgtacggc ttgttcgggt tgtccggcac
     1141 gacgctgtcg agcgcctcgt ccatgcggtc cgggtcgtcg gtcggctcga cccgcggcgg
     1201 cgtctggagg ttgttctgcg gcaggaacga gaacaggtag cgcgcgtcct cgaggcacgc
     1261 ctcctcgtcc tcggcggcga agtgggccac gcccgacttc gtgttgtgcg acatcgcgcc
     1321 gccgagctcc tcgaacgtcg tctcctcgcc cgtgaccgtc ttgatcacgt cgggacccgt
     1381 gatgaacatg tgcgaggtct ccttgaccat gaagatgaag tcggtcatgg ccggcgagta
     1441 gaccgcgccg cccgcgcacg ggcccatgat caggctgatc tgcgggatca cgccggagca
     1501 ctggacgttg cggacgaaca cgtcgccgta ggcgccgagc gagaccacgc cctcctggat
     1561 ccgcgcgccg ccggagtcgt tgatcccgat gacggggcag ccgatgcggg ccgccaggtc
     1621 catgatcttc accatcttct cggccatcac ctcgccgagc gagccgccga acaccgtgaa
     1681 gtcctgactg aagacgcaga ccgggcggcc gtcgatcaga ccgtggccgg tgaccacagc
     1741 gtcgccccag ggccggctct tctgcatgtc gaagtcgtag gtgcggtggc gcacgaaggt
     1801 gtcgagctcg gcgaaggtgc cggggtcgag cagcttctcg atgcgctcgc gcgcggtgaa
     1861 cttgcccttg gcgtgctgct tctcgaccgc ggcctcggag ccgctgcggc tcgcctcgcg
     1921 cagctcctgg agctgggcga gcttctcctc gaagctctcc ggatgcgact ggcgggccat
     1981 gggcggccgg gagtttacga gctcagtcgc gcgcgaacgg cttgcggtgt gcggagccga
     2041 ggctgtcgag cacgccctcc agcttctgct cgaggtcgtc gtgccgcgac gggagcggct
     2101 tggcgccgcg caggcgcgcc cagaaaccaa cctcggcgac ctgctcctcc accttcgcgg
     2161 agaggaccac cttggtgccc cagttcgacg gctcgatccg cgcggtgccg gctgcgccct
     2221 cggcgcgcca ggccagctcg cgctcgggtt cgcgcggctc gaccaccgcc tctcccacgg
     2281 cctgctggat gcaatcgccc tggagctcct cccagagctc gggaggcgac ttcacgagcg
     2341 tcctcgacac ctgcacgcgt ctcatcggcc ccgggtttcg cgcgccgcgg cggcgttcct
     2401 gccgaggctc agcgcgtggc cgtggcgctg ccggtgcgcg agccggcgcg ccagcgcagc
     2461 accaggcggc tgcccgagcg cgtcagcgcc ttccgggcgc tcctagacag gctcacgacc
     2521 gccttcgtcg tgcccgcacc gcccagcttc acggtcttcg tgccggccgt gctcttcttc
     2581 ttcccgcgcg tgaggacggc gcggagcttg accgagacgg ggccgtcgct gcggaccgtc
     2641 gcccgcaggc ggccggactt cgcgaggctc gcgcgggtgg tgcgcttgac ggtgacgctc
     2701 acctgaggct cggcggagcc gccgcccggg gccgggggcg gctgggaggg cgcgggctga
     2761 ctcggaccgc cggcgctcgg cgcgggcggc tggtaggccg gcgtcgtggc ggctgcgcag
     2821 cgcgcggtgg ccgcgtcggc gttcacgctc ccgtagccgt agaccgggtc gtaggcgccc
     2881 ccgttcgacg acgtcgtgcg caggcattcg acgatctgct cgttcgtcag gccgcgcccc
     2941 gccagcagcg cggccactcc ggacacgtgc ggggcggcca tcgagctgcc cgcgaacgtc
     3001 tcgtagccct tcaggtcgca gttggggttg ctgtcgaccg cgtcacccgg ccagaccgtg
     3061 gaccacacgt cggccgagta ctcgcagaag atcgggctgc cctcgccgcc gggggcccgc
     3121 aggccgagcg cgccagccgg ggagttcggg aagttcgagt agtaggacgg catgcccgcg
     3181 gagtcggtgg ccgcgacgca caccgcgtgc cgcgcggcgg ccgggtagtt gcagagcggg
     3241 aacgactcgt tgctcgccgc ggccaccacg acggcgccct tcgaccaggc gtggtccacc
     3301 gcccgctcgg cgtcctcgtc cgtcccgtcg accttcgcgc tcagcggcag atccgcgagg
     3361 ctcaggttga tcacgtcggc gccgttgtcg gccgcccagc ggatcgcgtc gtagacgacg
     3421 ccggtgtcgg cctcgccgcc ctcggcgtcg agcacccgca ccggcattac cttcgcgtcc
     3481 ggcgccacgc cggcgacccc ggtgccgttg cccgtaaccg ccgcgacgat gcccgccacg
     3541 tgcgtgccgt ggccgttctc gtcctgcggg ccggggcagc cgtcgtcctt cagcacgtag
     3601 tcggtgccgg ggagaagctt gccctggagg tcggggtgcg tcaggtcggc gcccgtgtcg
     3661 agcacggcga cggtgatgcc ggcgccgcgc gcgccgcggg cccacgcggc cggcgccttg
     3721 atctggctga ggccccactg cgccggggcg agcgggtcgc cggtggtggg gccgccgaac
     3781 gctgcaacgc cgccggcgcc ctgcgcgtag gccgaggcgg ggtgcgagca cgtgacggcg
     3841 ctcgccgagc cgggcgcgac gagcagcgca agggcgcaga cggccgcggc gatgagggtc
     3901 ctggcacgca cgaggtacgg gctcttgtac ccccggggga ccgcctccat gcgcccgact
     3961 gacgcaaccg gggcgccctt tggaggcgcc ccggtggtcg accctggaga acttctgcgg
     4021 aagcgagcga cggctcagtg cgctcaagtc tgtacacggg ttgtcgtcgg ccgcgcattc
     4081 tgccgatatg gggtgatgac cccatcttcc ggatgcgaac ccagagcaac tccaactcac
     4141 tgatcggcca ggtcgtcgcg accaacgtcc tgatggtcgt cgcgaccatc ttcgccgcca
     4201 gcgccgccgc caacctcgac ctcggggtgg gtgaccagcg cgcgcagttc ggcctgctcg
     4261 cgctgtcgat cgtgctggtg ctgctggtca acatgatgat gctgaggcgg cgcttctcgc
     4321 cgctcgaccg cctgatcgag cgcgtcgagg cgatcgaccc cgccaacccc gccgcgttcg
     4381 aggcgcccga gggctccgag gcggtcgagg agatcgaccg gctggcggcg tcgttccgca
     4441 acatgctcgg ccgcatcgac gccgagcgca ggcgcagcgg ccggctcgtg ctgcgcgccc
     4501 aggaggagga gcgccggcgc ctcgcgcggg acctccacga cgaggtcaac caggcgctca
     4561 cggccatcct gctccggctc gaggcgctga gccacgcggc gcccgaggtg ggcgacgagc
     4621 tctccgagct gaagaagctc gtgaaccagg cgatggcgga gctgctgcac ctcgcccgcc
     4681 agcttcgccc cacggcgctc gacgaccacg gcctcgtgcc cgcgatcgag agccagctgc
     4741 ggcgcttctc ggcccagacc gggatcaagg cggacatgcg cacggtggac ctccccggcg
     4801 tggtgcagtc cgacagcgag atcgtgatct accgcgtggc gcaggaggca ctttcgaacg
     4861 tcgcgcagca cgcgtccgcg ggccacgtcg agatcgggct gcacggacgc aacggccgtg
     4921 gtctcgagct ggtcgtgcgc gacgacggcc gcggcttcga cccggatgag acccgcgaca
     4981 ccctcggcct gctcggcatg gcggagcgcg cgcgcctcgt cggcggcaag ctcgacgtgg
     5041 cctcgacgcc cg
//
LOCUS       DASVPL010000048         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_318655, whole genome shotgun sequence.
ACCESSION   DASVPL010000048 DASVPL010000000
VERSION     DASVPL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318655"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VF517_02655"
     CDS             complement(<1..820)
                     /locus_tag="VF517_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051863.1"
                     /translation="MRTRQLLLLVLACAAVAPAGAHAATGAEAVARLNEQRAAHGIPA
                     GIAHNAEWSRWCGLHNEYQRINGGQLTHSEDPSKPGYTDEGNQAAGASVLSAGGNWDR
                     ANPWETAPIHLHQLLAPRLDAMGVDDTNGFTCATTLLSRNRPAPASNAVYTYPGPGTS
                     HRASETAAEGPYTPGERVGISKGTRTGPYIYVSVDGPQLNPFSKAHMTGASLSGPDGP
                     VEVRTVDNFTSGLEGYLPTGGQVIPVQPLKARSTYTASVNLSVTQGDATIPFERT"
     gene            899..1729
                     /locus_tag="VF517_02660"
     CDS             899..1729
                     /locus_tag="VF517_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX8051864.1"
                     /translation="MLEAFDAPYMQRALVAALLLSVPLGLLGTWIVLRGLAFFAHAVG
                     VATFPGLVVGLGVPAIGPFLGALGAAGAFSGSVTLLERDERVRGGAVTGLVLSTAMAL
                     GAVLLVSVFEETVPVEGVLFGSLLAVGDTDVVRAAVLAGAGTAALLLLGPRLAAGTFD
                     RTWAGPAGARTGLVDAGLLALTAVCVVVALPIIGSLLVSGMLVVPAATARLLSRGTGA
                     MLAWSVGLSACALVGGLIAAHALDAPPGGAAAALAGLGFAIAYTRDALARRPAAGAAA
                     "
     gene            1782..2627
                     /locus_tag="VF517_02665"
     CDS             1782..2627
                     /locus_tag="VF517_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX8051865.1"
                     /translation="MTADPRAASPPSPPRREPADGRPPTATLAVEAEGLGVTFGARPA
                     LVDVTFSIPAGSLVGVLGPNGAGMTTLLRALLGELPHSGRAELRGRPGYVPQHTAAHE
                     GFPIDALGVVLMGRYPRLGWLRRPTRADRTLALDLLDRVDLGEHARRQFSELSGGQRQ
                     RALVARALAQEGDVLLLDEPFTGVDAVSHEMLLTVLREQAAAGSTVLMTTHDVGEAGR
                     VCDRLLMLNRELIAAGPTAEIFTPETLRRAYGAQVLELGTGTVLLDEGVHHHHGDEPP
                     AGLTG"
     gene            2627..>2960
                     /locus_tag="VF517_02670"
     CDS             2627..>2960
                     /locus_tag="VF517_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX8051866.1"
                     /translation="MDWLTDPWTSELMRRAFAESVLVGATCGVLGCFVVARGLAFLGE
                     AVSHVLILGAVVALLAGGPVGLGAFAAAAVAVWLASIVSADRRFSPDTAIGILLPTFF
                     GLAVVLVAL"
BASE COUNT          278 a         1123 c         1105 g          454 t
ORIGIN      
        1 acgtgcgctc gaacgggatg gtggcgtcac cctgggtgac gctcaggttt accgaggccg
       61 tgtaagtcga ccgcgccttg agcggctgca ctgggatcac ctggccgccc gtgggcaggt
      121 agccctcgag cccgctcgtg aagttgtcca ccgtcctgac ctcgaccggg ccgtccggac
      181 cggacagcga ggccccggtc atgtgcgcct tcgagaacgg gttgagctgg gggccgtcga
      241 ccgacacgta gatgtacggg cccgtccgcg tgcccttgga gatgccgacc cgctcgcccg
      301 gtgtgtaggg gccctccgcc gcggtctcgc tcgcgcgatg ggaggtgccc gggccgggat
      361 aggtgtagac cgcgttcgag gcgggcgccg ggcggttgcg tgagagcagc gtggtggcgc
      421 aggtgaaccc gttcgtgtcg tcgacgccca tcgcgtcgag gcgcggtgct agcagctggt
      481 gcaggtggat cggcgccgtc tcccatgggt tcgcacggtc ccagttgccg ccggccgaga
      541 ggacgctcgc tccggccgcc tggttgccct cgtcggtgta gcccggcttg ctcgggtcct
      601 cggagtgggt cagctggccg ccgttgatgc gctggtactc gttgtggagc ccgcaccagc
      661 gcgaccactc cgcgttgtgg gcgatccccg cggggatgcc gtgggccgcg cgctgctcgt
      721 tcagccgggc caccgcctcc gcgccggtgg ccgcgtgcgc accggccggg gccacggccg
      781 cgcacgcgag cacgaggagg aggagctgcc gtgtgcgcac cgatctcgac cgtagtggcc
      841 gggggcgggc ggcgccagcc ttcgccccta aacgcgaatc gttatcgttt tcgtcagcat
      901 gctcgaggcc ttcgacgcgc cctacatgca gcgcgcgctg gtcgcggcgc tgctgctttc
      961 ggtcccgctc gggctgctcg gcacgtggat cgtgctgcgc gggctcgcgt tcttcgcgca
     1021 cgcggtaggc gtggcaacct tccccgggct cgtcgtgggg ctcggggtgc cggcgatcgg
     1081 gccgttcctg ggagcgctgg gggccgcggg cgccttcagc ggatcggtga cgctgctcga
     1141 gcgcgacgag cgcgtgcgtg gcggcgcggt caccgggctc gttctctcga ccgcgatggc
     1201 ccttggcgcc gtgctgctcg tgtcggtctt cgaggagacg gtgccggtcg agggcgtcct
     1261 gttcgggtcg ctgctcgcgg tcggcgacac cgacgtggtc cgggcggccg tgctcgccgg
     1321 cgccggcacc gcggcgctgc tgctgctcgg gccgcgactg gccgcgggca ccttcgatcg
     1381 cacctgggcc gggccggccg gcgcgcgcac cggcctcgtg gacgcgggcc tgctggccct
     1441 caccgccgtg tgcgtggtgg tggcgctgcc gatcatcggc tcgctgctcg tctcggggat
     1501 gctggtggtc ccggcggcca ccgcccggct gctcagccgc ggcaccggcg cgatgctcgc
     1561 gtggtcggtg gggctgtcgg catgcgcact cgtgggagga ctgatcgcgg cccacgccct
     1621 ggacgcgcct ccgggcggag ccgcggccgc cctggcgggc ctcggcttcg cgatcgcgta
     1681 cacccgcgac gccctggcgc gccgcccggc ggcgggagcc gcggcgtgat ggctgacccg
     1741 cgcgatgcgc tggcgcgccg cccggcggcg ggagcggccg cgtgacggcg gacccgcgcg
     1801 ccgcgagccc gccctcgccc ccgcgccgcg agcccgccga cggccggccg ccgaccgcca
     1861 ccctcgccgt cgaggccgag ggcctcggcg tgaccttcgg cgcgcggccc gccctcgtcg
     1921 acgtcacgtt ctccatcccc gccgggtcgc tcgtcggcgt gctcggaccc aacggcgcgg
     1981 gcatgacgac ccttctgcgc gccctgctcg gtgagctccc ccactccggc cgcgccgagc
     2041 tgcgcggccg ccccggctac gtgccgcagc acaccgcggc ccacgagggc ttcccgatcg
     2101 acgcgctcgg cgtcgtgctc atgggccgct acccgcggct cggctggctg cgccgcccga
     2161 cccgcgccga ccgcacgctc gcgctcgacc tgctcgaccg ggtcgacctg ggcgagcacg
     2221 cgcgccgcca gttcagcgag ctctccggcg gtcagcgcca gcgcgccctc gtcgcgcgcg
     2281 ccctcgccca ggagggcgac gtgctcctgc tcgacgagcc gttcaccggg gtcgacgccg
     2341 tctcgcacga gatgctcctc acggtgctgc gcgagcaggc ggccgccggc tcgacggtcc
     2401 tcatgaccac ccacgacgtc ggcgaggccg gccgcgtctg cgaccgcctc ctgatgctca
     2461 accgcgagct gatcgccgcc ggcccgaccg ccgagatctt cacccccgag acgctccgcc
     2521 gcgcgtacgg cgcgcaggtg ctcgagctcg gcaccggcac ggtcctgctc gacgagggcg
     2581 tgcatcacca tcacggggac gagcccccgg cgggcctgac cggctgatgg actggctcac
     2641 cgacccctgg acctccgagc tcatgcgccg cgcgttcgcc gagtcggtgc tggtgggcgc
     2701 cacctgcggg gtgctcggct gcttcgtcgt ggcccgcggg ctcgcgttcc tcggcgaggc
     2761 ggtctcgcac gtcctgatcc tcggcgcggt ggtcgcgctg ctggcaggcg ggcccgtcgg
     2821 cctcggcgcg ttcgccgcgg ctgccgtggc ggtctggctc gcgtcgatcg tctccgccga
     2881 ccgccgcttc tcccccgaca ccgcgatcgg gatcctgctg ccgacgttct tcggcctcgc
     2941 cgtcgtgctc gtggccctga
//
LOCUS       DASVPL010000049        24537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_162435, whole genome shotgun sequence.
ACCESSION   DASVPL010000049 DASVPL010000000
VERSION     DASVPL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24537
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162435"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VF517_02675"
     CDS             complement(<1..282)
                     /locus_tag="VF517_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX8051867.1"
                     /translation="MRILVAGGGIGGLAAAIACSEAGHEVEVFERAKELTDVGAGLTL
                     WANGLQALRRLGVDLPPPQRPIRRLRMTRAGGGDFATIDVAKLEARHGAG"
     gene            complement(294..1082)
                     /locus_tag="VF517_02680"
     CDS             complement(294..1082)
                     /locus_tag="VF517_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051868.1"
                     /translation="MELRGRRILLTGASGGIGQAIARRLSADGARLVLTGRRRDVLEG
                     LAAELGDAEARPADLTDRDALAELAGLDLDGFVSNAALPGSGGLDEYSLDQLDRVLDV
                     NLRAPVVLARLLAPRLRERGSGHIAFVTSVSARMPAAWASLYCTTKFGLRGFALSLRQ
                     ELRGTGVGVSLVLPGMVRDAGMFAEAGVDPPRGAGTSSPEEVAAAVVRAIERDRAELT
                     VAALAVRLNMELASAAPELAGMIGSRLGADDFMAALARGQRHKR"
     gene            complement(1088..1942)
                     /locus_tag="VF517_02685"
     CDS             complement(1088..1942)
                     /locus_tag="VF517_02685"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8051869.1"
                     /translation="MTRRVLITGASTGIGATTALRLADRGWKVLAAVRRAEDGERLVA
                     RGGANVSWLRLDVTDAAEIEAAAAQVEAAGGLDALVNNAGIVVPGPLETLPIDDLRRQ
                     LEINFVGHVAVTQALLPALRRSRGRIVFMGSIGGRVGQPFISAYTASKHALEAVADAL
                     RVELAPWGIRVSLIEPGSVRSETWGKSAAYFDDLFEGLPAEQRELYRKPVGRVRAVAR
                     MQERGAVPATWVARAVTHALESGRPRSRYLVGPEAWSLRAFRVLPDRARDAIVVGALG
                     LRRGAAGG"
     gene            1974..3371
                     /locus_tag="VF517_02690"
     CDS             1974..3371
                     /locus_tag="VF517_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8051870.1"
                     /translation="MSASVASRASAQGRLTLLDGWGRLTPSAAFVARPHRPGEVDALL
                     ANPPRGGLIVRGLGRSYGDAAQNTGGLVVDARSLDRILDLDLGAGTVTAEGGVSLDRL
                     MRVLLPLGRFVPVTPGTRFVTLGGAIAADIHGKNHHRDGAFCEHVTEIVLRTPRGRIV
                     VAPDRDPDVFWATAGGMGLTGLIESATIAIPPVETAWIRADHERARDLDDCLGRLDSG
                     DRRYQYSVAWIDCRARGRRLGRSVLERGDHAAVADLDGPAAADPLRFEPATQLAAPPW
                     PPGGVLRPLTIPALNEAYFRRVPREERDRLVPLASFFHPLDGVAAWNRLYGSRGFAQL
                     QALFPFGAEAELRETLERLAGARAASSLALLKRFGAAGAGPLSFPGPGWSLATDVFAH
                     AADGGLLDRLHEVVAGCGGRVYLAKDSRLRPAAAAAMYPRLGELNAVRTRLDPDGVLR
                     SDLSRRLALGGGVPA"
     gene            3368..3853
                     /locus_tag="VF517_02695"
     CDS             3368..3853
                     /locus_tag="VF517_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051871.1"
                     /translation="MSATRPTPLQPREFFTPPWSGTGRWTPRAAISPFAPARRFAFRT
                     FTTFLSDELWLIHDETAWEDGRVERRDGMAVLLAPDRFRLTYDGMPGGAEITLRADGF
                     EASPYSLSVDVHPLPLSMTVRCTDACSLRPDGELVDVIDVALLGVRLGRVTMSLRAET
                     G"
     gene            3911..5440
                     /locus_tag="VF517_02700"
     CDS             3911..5440
                     /locus_tag="VF517_02700"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX8051872.1"
                     /translation="MRFLGGSAATARDPDAIPTGRFQRAAATSAVLGPRSMRFLASSI
                     TSIARSPGRPEGPQGGSLVSHADEGMQRRCAEFADRTREVLGHARGGVMKVGQVASFI
                     DAQFLPPELRAIYQQRLAALRDGAPPMSWARVRCVIESELGARVESLFEEFDHDAAAA
                     ASIGQVHRAVLPGGRVVAVKVQYPDIEHALDADVQTAASLIPLSKPLLPGLDAQPLVR
                     ELRERVLEELDYELEARHQRLFADAYRGHPFIHVPDVVDGLSRRRVLVTDWVDGLGFE
                     EMCALPQAERDRVGEIIERFYYVPIYRLGRFNTDPHPGNYLLRPDGRVAFVDFGSVRA
                     IDPRQVRELVSSIEAALDGDARRLARRFAAMGFLDWPDEIDADALLERTLASGDWYLR
                     DRELRMDPDYVARCVAALLDPRSVRGGLELARHTNLPPHHLWLLRVELGVLSVLGHLR
                     ARGNWHRVARELWFSEESETELGRAEQAFFRGPAGSARPARSAPRRAASPARTPAAAT
                     A"
     gene            complement(5349..7115)
                     /locus_tag="VF517_02705"
     CDS             complement(5349..7115)
                     /locus_tag="VF517_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8051873.1"
                     /translation="MAGGTDTTRSLTRRGLLGSAAAAGAAGALARPGRAHAARRDGRS
                     VAVLGGGIGGLSAAHELVERGFRVTVYEHGALGGKARSIPVPGSGSGGRRDLPGEHGY
                     RFFPGFYRHLPDTMRRIPFGKNAEGVFENLVEASQAGFARNGGREDLIIPLAVHPWTP
                     QEAAQAVVAGFAQISNVPPEESAYFGRKLSVFMSSSDERRLGEWERQSWWNFVGADRF
                     SDEYKRFFGAGIARNFAAAKPESASARTIGLMAEQIVYQNAMKPRGEYQGADRVLNGP
                     TSEAWLDPWVAELRRRGVTFKLRRRVMGLDVRKGKIAGVRLRGPRGDEVARADWYFCA
                     LPVEQARKLWSPAVLAADPRLAGTGSLVTDWMTGIQFYLDRELPILHGHVSYVDSPWA
                     LTSISQQQFWTHDFAKDGDGRVRDCLSVVISDWHTPGILYGKPARELRPEQIAEEVWA
                     QIKGHLNDTGRDALPDGARKSWFLDPAIGYRPGDRVRARNATPLLINTVETWAKRPDS
                     VTGIHNLFLASDYPQTNTDIACMESANESARRAVNGLLARAGSQVDPCAIFKLWQPPE
                     FEPAKQLDAERYARGEPNLLDL"
     gene            7181..8530
                     /locus_tag="VF517_02710"
     CDS             7181..8530
                     /locus_tag="VF517_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8051874.1"
                     /translation="MTRVNVSGSFIAPALVRRPAAEGLPAGPWLPELIQSAQYGLDPY
                     GFFFRAHRRFGDAYTVRVLGQTWVVVAHPDSVRELFQLGPEDVNSGETNVDLRPILGT
                     GNVGLLDGDEHLQRRRIVLPSLQRERMPAYAEMVGEVAGRRIAELPVDTPVSVLPRMR
                     RITFDVLLRGVLGAEDRAAVDRIDAELESLLAYSMNPVRGAVFALLGPEGLARMPGFR
                     RRHAALDEDVFGEIRRRRDDPRLGEREDLLSRLLRIRDERGVALPDEELRDEIVTLLV
                     AGQETTASLLSWAVHELARAPEAQERLAAGEEGWAEAVIRETLRLHPPIVIILRRLRH
                     ELRVAGHDLPTGTTIAIAALLVHRRPDLYPEPDAFRPERFLGKRTTPGDWMPFGGGAR
                     RCLGAPLAQLEARMVLEQLAREHTIRPARARRERAGRRGIVTVPGRGGRVVLSRRSA"
     gene            8532..10328
                     /locus_tag="VF517_02715"
     CDS             8532..10328
                     /locus_tag="VF517_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8051875.1"
                     /translation="MTAPANDSGLSRRRFIRDTAVAGAGLAALPGASAAAARPKRRTR
                     RTVAIFGAGVGGLTAAQEMRERGFDVVVYERKALGGKSRSNTVPGSARGGRRELPGEH
                     GLRFVPGFYHNLPDTMRRIPVAGNENGVEGNLIATRSSSFARDGGREDLVFPPDSQEA
                     SGESLFQWLNGGFTQGSNIPPQEIAYLSNRLAIFFSSSDERRLGQWEHVSWWDYLNAD
                     RRSEEYRRLLVNGVTRNFVAAKPQLASTRTIGRIFEAFAYTSMRRDGGGEFDRVLNAP
                     TNEAWIDPWVKHLRKLGVRFRSGTVRSLALKRGRIASACVRDSKGRTRTVDADWFVCA
                     MPGDRARSLWTPALRRADPRLDAMRTLQTAWANGIQFYLRDEVPVLHGHISYVDSPWA
                     LTSLSQAQMWEGRDFARDYGDGSVRDCLSVTLSDWDDPGILFGRPASRCTPDEISQEV
                     WAQVKAHLEDTGRTYLPEGVLHSYHLDPAISYKPRRPGDGGVRARNDEPLMLNAVGSW
                     SSRPEARTAIPNLFLAADYVKVDIDLATMEGANEAARAATNALLDASGSNAPRAEIRE
                     LYRPPEFEEAKRQDAERFARGEPHVLDVPEPT"
     gene            complement(10329..11096)
                     /locus_tag="VF517_02720"
     CDS             complement(10329..11096)
                     /locus_tag="VF517_02720"
                     /inference="COORDINATES: protein motif:HMM:NF022696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3050 domain-containing protein"
                     /protein_id="HEX8051876.1"
                     /translation="MPVTYSADVEVVRARLIDHELYGRLNSVEAVQIFMKHHVFAVWD
                     FFSLLKRLQRTVTCVDVPWQPPANPTYARLINSIVLAEESDEDEQGNHGSHFELYLEA
                     MGELGADTGPIHAYHARLAAGEDPIEALSTAGVPGRVDEFVTHTLETALEGADHEVAA
                     SFCYAREDVIPDMFEPIVASLKAGGHSIPRLSYYLDRHITLDHDEHGPLAQQLLNSIC
                     GDDETLRAEADVAAVRALEARSALWDGVLEEVEEAGV"
     gene            11512..12315
                     /locus_tag="VF517_02725"
     CDS             11512..12315
                     /locus_tag="VF517_02725"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX8051877.1"
                     /translation="MRDLVERRRPDLLALAVELVGADPFERIDAGTAYLQPAIYCTTI
                     ASLHAVRALEPDLYAGHSLGELAALAAAGSISEDDGLRLVAARGRLMQRAAEAEGDGA
                     MLAVGAGIDAATELAHAYGLTVANDNSPEQTVLSGPAAAVTDARDDVKARGLRAFRLP
                     IDGAFHSPAMAAALPEFRALLDATEVRPPRRPVFSCVTAQEFDDVRARLAESLVRGVR
                     WREVLLALHARGADRFVEVGPGQVLTKLARATLPGVDALPAEELEAVGA"
     gene            12308..13315
                     /locus_tag="VF517_02730"
     CDS             12308..13315
                     /locus_tag="VF517_02730"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX8051878.1"
                     /translation="MPDAAAGAVAEPLRPPRATRGAGIAALGVALPRTAVPNAPIAER
                     LGVDDHWIVKRIGVHERRMVQDDERLSDLVERASRQALERAGVEPDQLDLILVGTFTQ
                     DEILPNAAPLTAGALGATRAGAVDLGAACMGFLSGVALAAAQIEAGRADTVLVVGAEV
                     LTRFLDMDDRRTAALIGDGAGAVVMTAIDGPSRIGPCILRADDRSREVVYMTREDGKL
                     RMEGQQTFAIAVDSLSDVSRQAVAAAGMTLDDIDLFVYHQANARITKAVGERLGLRGE
                     RVVDCIEALGNTSAASIPLALGVAAGEGRLHDGDRILVAAIGAGFTWGAAVIEWSGRA
                     V"
     gene            13305..14039
                     /gene="fabG"
                     /locus_tag="VF517_02735"
     CDS             13305..14039
                     /gene="fabG"
                     /locus_tag="VF517_02735"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX8051879.1"
                     /translation="MPSDGGCALVTGASRGIGAATARVLAGEGWPVGVNYRTDEDGAR
                     AVVEEIEREGGEAVVAQADVSQSAAVDEVFRALEERYERVAVLVNNAGTRADGLSVQL
                     DDEQWDTVMSTNLSAAFWTCRRALMPMVRSRFGRIVNIASVVGPCVANPGQANYAAAK
                     AGLVGLTRTVAREVAHRGVTVNAVAPGFVETQLTGDVSPDFIKAVPARRAGKPEEVAA
                     CVRFLASRDATYVTGALLTVDGGMTA"
     gene            14062..14313
                     /locus_tag="VF517_02740"
     CDS             14062..14313
                     /locus_tag="VF517_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX8051880.1"
                     /translation="MPSTETDPQIVEEAIIESIAYLGAPRADIKREATFEELDLDSLD
                     LVEIAQMAEEKWGASLEPQDLGGVKTVGEAIDLVIARLP"
     gene            14310..15515
                     /locus_tag="VF517_02745"
     CDS             14310..15515
                     /locus_tag="VF517_02745"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX8051881.1"
                     /translation="MSRRVVITGVGAVTPLGVGARTLLRRWAEGQSGLEGGLGRCDEF
                     DPEQSLSRKEAHRSDRFTQLALTACAEALADAGWDDGPPCPPERAGCVMGTGIGGIAT
                     IEVQHDTLRERGQKSLSPFGVPMMMGNAAAGALAMRHGLTGPNWGVVSACAAGAHAVG
                     SAVRLVQSGEADAVVTGGTEAALTPVAMGLFARMGATSPTGVSRPFDARRDGFVLGEG
                     AGVLVLEEAEAARARGAATLGEVLGYGATADAYHLTAPDPAGDGAARAIRAALDDAGI
                     EPEALDYVNAHGTSTPLNDRSETAALKAALGDHARRVPVSSLKSAIGHLCGAGGAVEA
                     IATVLALRERVAPPTLNWSEPEEGLDLDYVPEHARPLRDGANGGRAVAISNSFGFGGH
                     NAVLCLAAD"
     gene            15518..16012
                     /locus_tag="VF517_02750"
     CDS             15518..16012
                     /locus_tag="VF517_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX8051882.1"
                     /translation="MAAGTETARAALGCYFDELEAGQGFGSRGRTITEADIVAFSALT
                     GDWHPQHSDAVWAAASGFGERIAHGMLVLSFALGLVELDPDRVVALRRVRTATFKAPV
                     AIGDTIRVEGRLGGMREVDGERGLVETSLRVVNQHGRAVASFSLDLVWRRSEARPREA
                     KAIT"
     gene            16009..17196
                     /locus_tag="VF517_02755"
     CDS             16009..17196
                     /locus_tag="VF517_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051883.1"
                     /translation="MRAAAPRATSPAPGPLAIVGGYGLVGAQAARLLRERHPDLPLVL
                     AGRRPATARELAASLRATTARVDVGEERPLRSLPHRPAAVLAAVSDPHDHLLADAMRA
                     GIPIADINRGGAAAVLDAFVRATTERPRAPVLLSGSWLSGLTALVAAAAVREIGGAER
                     VEVAVLVSSGDRVGDDAWGFSKRLAFPYHAMAGGRRQAVQPLTGVRHVRCADGRERPA
                     ARVGTLEQITLPIALGVPTVETRIAPHDAAALWALVGLKRSGALRTLELPALRRVRAA
                     LLHRSGPGDFAGFGVTAHGDGCHTTIEVLDVRGQSHLNAVGAARAAERVLGLAGAELT
                     PGVSFPEQSADPPADLAALSEAGVVVRRGGFRRPAPAPGPPATDADLVFMPTEIPEEN
                     LTR"
     gene            17193..17561
                     /locus_tag="VF517_02760"
     CDS             17193..17561
                     /locus_tag="VF517_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051884.1"
                     /translation="MSSRRARAPRLTLSILRLVGGAAFLLPRVGVKQLGLRDDAESAY
                     LMRLFAARNVAMTTGLLLSRGDARRLWWQVGIVCDVLDAGAGLLGMREGKERTSAVVD
                     TGASLVATGLGVAGLIADRG"
     gene            17571..18917
                     /locus_tag="VF517_02765"
     CDS             17571..18917
                     /locus_tag="VF517_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8051885.1"
                     /translation="MTTIERTPSRSDDGASARAERVCVVGAGSAGIAMCRALAVRGIP
                     FDCYERTDAVGGLWRYPPAGGGSCAYASLYPNTSKTVMQFPSYPMPEDYPHYPHHTAV
                     ARYFDDYVDHFGFRDAIRFGTEVTSVAPAAGGGWDVTLGDGTTHPYRAVAVASGGRHA
                     EPVYAELEGEFEGRQLHSFDYDGPADFRDRRVVVLGLGATSADIASELSLVARATYLS
                     VRTGHYVVPKLIEGRPIDVLSPLMKRLSVEQRRPLLTFLLKFVNGSPTDFGLPKPPYK
                     PGQGPLIATTDLLPAIAHGRVVPKPVVATVAGSTVRFADGSEVEADAIVHCTGYRIGF
                     PFLDRSLVSGGDDAPPLYRLVVAPELDGLYFIGLLHSMMSLMPLAEAQSEWVAEVLEG
                     SVALPPRERMWSEIRRERRRQDRRFHDSSGHLLVDPNEYERLLARERAKHGAPARL"
     gene            18955..19362
                     /locus_tag="VF517_02770"
     CDS             18955..19362
                     /locus_tag="VF517_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051886.1"
                     /translation="MKNEEQQPSRGGRPGGLDLSRLSQAELAELKEEIIRRQRPAGSR
                     LEPTMATTDDGSFFFTDDRLAWDDRWVTVIDAISQGVTRIAAAQIAAGSAMASSQMMR
                     AGNDRMAKATLGVAAAAAAGLAGTALRDARRQR"
     gene            19407..20405
                     /locus_tag="VF517_02775"
     CDS             19407..20405
                     /locus_tag="VF517_02775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8051887.1"
                     /translation="MIARDAAATLPACLASVAAVADEMVVVDTGSSDDTAAVAREHGA
                     RVIPFRWCDDFAAARNAYVRAARGSWVLSLDADEVLDPVDREGLRAVLAANPRTACVV
                     TVRNYFALGELNWPYMPSEFLGEVSPGIGCMTSRTIRLFPRATLPEYRFPVHESLVPS
                     LRHRGVAIRHCRLGIHHTGYLGGRGEFEARAALYGRLGERKLELFPRHPLAHLELGRV
                     RLFEGEIDDAARLFRNCIRLRPRLVPAHFFYVLALAHGGHSGEAHGHLARALELMPGD
                     PDLLYLRGLLALESAGPEAVAADLAPALRRMSAGAPERAQMPPRVLASASSRSDAP"
     gene            complement(20351..22198)
                     /locus_tag="VF517_02780"
     CDS             complement(20351..22198)
                     /locus_tag="VF517_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051888.1"
                     /translation="MAVVFLALNGVGLGHLVRATTVCDALAQTGERPVVFSQGIFPLD
                     ERSEHPGKVVPSLWGTDEPVRRRVSGEIASMAALSLPAVLIEDTHPNPLTLPQEIRRA
                     LVVRPTTFAYLQDLNDHYSSVYSSFVLCDAAGSPTWPYSESETRVLSQWPQWGQVGPV
                     YRRASEADADEVRRRHGIDGERRLCVFSMGGGGLHRSTDPDAVEFARLAGEVADAILA
                     VDPSARLMFVRGPYFPDGVELDPRLEVVDQEPRMPALLSLAHAAVVRSGFNTIWECVA
                     GGVPFLPFVGSTFAEPNDERLARLRAEGLLCDDPQRLWWDEDWRASFRERCRAITARN
                     PGRPDAARLRTLLVGERRRVRVRPPRPAPVRGRSALRTPRRRRPARAGSPLAIRVDDV
                     AVPELTLGWLLRILAERRLRASLDVVPYLNELNEASLAPFDPGGDLFEVAQHGYAHVP
                     HSDAAGRRHEFAPDSERPAPDEVRLIRRGRAELLAAFPDRFGGGLSPPFDALPLWLAD
                     VWHGAGGLFVSHLFDRSLPPVPIPVVRAGTDIWDWDRARPRPHQAILEAIEADRARHG
                     HAGIVLHPRCLRRRSAQEHLVTLLDALAARGFYGASLRELALASTRGGI"
     gene            complement(22201..24192)
                     /locus_tag="VF517_02785"
     CDS             complement(22201..24192)
                     /locus_tag="VF517_02785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8051889.1"
                     /translation="MSRPRVALLVTDRRLMPAWTGNRVRILGVVRALRAQGLRVVVIG
                     AQDPSLHDVRPLVDGVIAVRARPFQGGPIAAFDPRPFRRAVDRVARRFEPEVAIAEYA
                     WLAPALSGLPRGTRRWVDCHDVLHERCARFQAAGLDPWTVCTLEEEVERLRTADVLIV
                     SQHRDEELLGSLLPHREVVTLLPEIPLPDGYRPAAAGGRTVLAVGSAQPGNEMVREFA
                     RVGWPRVLSRVPDARLRVIGAVGEGLAGLEGVDAPGDVDDLAGEYARAAAVVCPVAVG
                     SGVKVKLLEALRLGRPTVATPLASEGLPRATLAWAEAESVAGCADAVAGLLLAPASAA
                     AHGRAAFAFGERHLSATAASACLRPLLESPSATRASRVRGLPVASASVIVPATSWSDP
                     LRACIESLQAQRVEQPVEIVVVMNGADPPAERTLPGVSVVHEPRRGPAAARNAGVRAS
                     SGDVLAFVDSDCVARPGWLASGLATMRTGAPGGIVAGAIVRPDAGRNPVSLYDSVTYL
                     QQERYVNGPGAFVTANLLVHRRTFERVGPFDASFEEAAFEDWEWALRARRAGLPITYE
                     AAAAVEHPCMTTLDELRQKAERLARGRLVMHRKLGEDAEALTLTESVWSHARRALREE
                     RVARGDRLRVAGVGSLVGFWAWSTDREQRRLHDRADRRG"
     gene            complement(24189..>24537)
                     /locus_tag="VF517_02790"
     CDS             complement(24189..>24537)
                     /locus_tag="VF517_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051890.1"
                     /translation="TFETSQVVAGYGDSFRLEVSGDGGTLIVDSERDGEIRLLSGEAL
                     ERRATWTTDIPARALPADLALSGAPASPGAIVEALRGERVEFPTFADGVAAQRVLGAL
                     LESTRTGSWSAVG"
BASE COUNT         3094 a         9248 c         9087 g         3108 t
ORIGIN      
        1 ccccgcgccg tgccgcgcct ccagcttggc cacgtcgatc gtcgcgaagt cgccgccgcc
       61 ggcgcgcgtc atgcgcaggc gccggatcgg ccgctgcggc ggcggaaggt ccacgccgag
      121 ccggcgcagc gcctggagcc cgttggccca cagcgtcagc ccggccccca cgtcggtgag
      181 ctccttggcg cgctcgaaga cctcgacctc gtgaccggcc tccgagcagg cgatcgcggc
      241 cgcgagcccg ccgatgccgc cgcccgcgac gaggatgcgc atccgccgcg cgctcaccgc
      301 ttgtgccgct gcccgcgcgc gagcgccgcc atgaagtcgt cggcgccgag ccggctgccg
      361 atcatccccg cgagctccgg cgccgcgctc gccagctcca tgttcagccg cacggccagc
      421 gcggccacgg tcagctccgc gcggtcgcgc tcgatcgcgc gcaccaccgc ggccgcgacc
      481 tcctccggcg agctggtgcc cgccccgcgc ggcggatcga cgcccgcctc ggcgaacatc
      541 cccgcgtcgc gcaccatccc cggcagcacc agcgacaccc ccaccccggt gccgcgcagc
      601 tcctggcgca gcgagagcgc gaagccgcgc agcccgaact tcgtggtgca gtagagcgac
      661 gcccacgcgg ccggcatccg cgccgacacc gaggtgacga acgcgatgtg cccgctgccc
      721 cgctcgcgca gccgcggcgc gagcagccgg gccagcacga ccggcgcgcg caggttcacg
      781 tcgagcacgc ggtcgagctg gtcgagcgag tactcgtcga ggccgccgct gccgggcagc
      841 gcggcgttcg atacgaagcc gtcgaggtcg aggccggcca gctcggcgag ggcgtcgcgg
      901 tcggtcaggt ccgccggccg cgcctccgcg tcgcccagct cggcggccaa cccctcgagc
      961 acgtcccggc ggcggccggt gagcaccagc cgcgcgccgt cggcgctcag cctccgcgcg
     1021 atcgcctggc cgatgccgcc ggaggcgccg gtgaggagga tcctgcggcc gcgcagctcc
     1081 atcccgctca cccgcccgcc gcgccgcggc gcagccccag cgcgccgacc acgatcgcgt
     1141 cgcgcgcccg gtccggcagc accctgaacg cgcgcaggct ccaggcctcg gggccgacca
     1201 ggtagcggct gcgcgggcgg ccggactcga gcgcatgcgt gacggcgcgt gcgacccagg
     1261 tggccggcac cgccccgcgc tcctgcatcc gcgccacggc ccgcacgcgg ccgaccggct
     1321 tgcggtagag ctcccgctgc tcggccggga gcccctcgaa caggtcgtcg aagtacgccg
     1381 ccgacttgcc ccaggtctcg ctccggaccg aacccggctc gatcaggctc acgcggatgc
     1441 cccacggcgc gagctccacg cgcagcgcgt cggccaccgc ctcgagcgcg tgcttgctcg
     1501 ccgtgtaggc ggagatgaac ggctgcccga cgcgcccgcc gatcgacccc atgaacacga
     1561 tccgcccgcg cgagcgccgc agcgccggca ggagcgcctg cgtgaccgcg acgtggccga
     1621 cgaagttgat ctcgagctgc cggcgcaggt cgtcgatcgg gagcgtctcg agcgggcccg
     1681 gcacgacgat gccggcgttg ttcacgaggg cgtcgagccc gccggcggcc tcgacctgcg
     1741 cggccgcggc ctcgatctcg gcggcgtccg tcacgtcgag gcgcagccag ctcacgttcg
     1801 cgccgccgcg cgcgactagc cgctcgccgt cctcggcccg ccgcaccgcg gcgaggacct
     1861 tccagcctcg gtcggccagg cgcagcgcgg tggtcgcgcc gatgcccgtc gacgccccgg
     1921 tgatgagcac gcggcgggtc accgcgcgcg ggtgcggggg tagagtgcgc gcgatgagcg
     1981 cgtccgtcgc gtcccgtgcc tccgcgcagg gacggctgac cctcctggac ggctggggca
     2041 ggttgacgcc gagcgccgcg ttcgtcgcgc gcccgcaccg tcccggcgag gtcgacgcgc
     2101 tgctcgcgaa cccgccgcgc ggaggcctga tcgtgcgtgg cctcggccgc agctacggcg
     2161 acgccgcgca gaacaccggc gggctcgtcg tcgacgcgcg ctcactggat cggatcctag
     2221 accttgacct gggcgccggg accgtgaccg cggagggcgg cgtgagcctc gaccggctga
     2281 tgcgagtcct cttaccgctg gggcggtttg tgcccgtcac gcccggcacg cgcttcgtca
     2341 cgctgggcgg cgcgatcgcg gccgacatcc acggcaagaa ccaccaccgc gacggcgcct
     2401 tctgcgagca cgtcaccgag atcgtcctgc gcacgccgcg cggccggatc gtcgtggccc
     2461 ccgaccgcga ccccgacgtg ttctgggcga cggcgggcgg gatgggactg accgggctga
     2521 tcgagagcgc gacgatcgcg atcccgccgg tcgagaccgc gtggatccgc gccgaccacg
     2581 agcgtgcccg cgacctcgac gactgcctgg gccggctcga ctccggcgac cgccgctacc
     2641 aatactcggt cgcgtggatc gactgccgcg cgcgagggcg ccggctcggc cggtcggtgc
     2701 tcgagcgcgg cgaccacgcc gcggtcgccg acctcgacgg cccggccgcc gccgacccgc
     2761 tgcgcttcga gccggccacc cagctcgccg cgccgccgtg gccgcccggc ggcgtgctgc
     2821 ggcccctgac gatccctgcg ctcaacgagg cctacttccg ccgcgtgccg cgcgaggagc
     2881 gcgaccggct cgtcccgctg gcgtcgttct tccacccgct cgacggcgtt gccgcatgga
     2941 accgcctcta cggcagccgc ggcttcgccc agcttcaggc gctgttcccg ttcggcgccg
     3001 aggcggagct gcgcgagact cttgaacggc tggccggcgc ccgcgcggcg tcgtcgctcg
     3061 cgctgctcaa gcgcttcggc gcggccggcg ccgggccgct ctcgttcccc ggccccggct
     3121 ggagcctcgc caccgacgtg ttcgcgcacg cggccgacgg cgggctgctc gaccgcctcc
     3181 acgaggtcgt cgccggctgc ggcgggcgcg tctacctcgc gaaggactcg cgcctgcgcc
     3241 cggcggccgc ggccgcgatg tacccgcggc tcggcgagct gaacgccgtc cgcacgcggc
     3301 tcgacccgga cggcgtgctg cgctccgacc tgagccgccg cctcgccctc ggcggtggag
     3361 tgccggcgtg agcgcgaccc gccccacccc gctccagccg cgcgagttct tcacgccccc
     3421 gtggtcgggc accggccgct ggacaccgcg cgcggcgatc tccccgttcg cccccgcgcg
     3481 ccgcttcgcg ttccgcacct tcaccacgtt cctctccgac gagctctggc tgatccacga
     3541 cgagaccgcg tgggaggacg gtcgggtcga gcgccgcgac ggcatggcgg tgctgctcgc
     3601 ccccgaccgc ttccggctga cctacgacgg catgcccggc ggcgccgaga tcaccctgcg
     3661 cgcggacggc ttcgaggcct cgccgtactc gctgtcggtc gacgtgcacc cgctgccgct
     3721 gtcgatgacc gtgcgctgca ccgacgcgtg cagcctgagg cccgacggcg agctcgtgga
     3781 cgtgatcgac gtggcgctgc tgggcgtccg cctgggccgc gtgacgatga gcctgcgcgc
     3841 cgagacgggc tagcgcgccc gcctccgaat cggaggattc gagctggcgc cgaggggaaa
     3901 caacgcgcac atgaggttcc tggggggcag cgccgcgacg gcgcgggatc cggacgcgat
     3961 cccgactggg cgattccagc gcgcggcggc cacctccgcg gtgctcgggc cgcgctcgat
     4021 gcgcttcctc gccagctcga tcacgagcat cgcccgctcc cccggccgcc ccgagggtcc
     4081 gcagggcggc tcgctcgtct cccacgccga cgagggcatg cagcggcgct gcgccgagtt
     4141 cgccgaccgc acgcgcgagg tgctcggcca cgcgcgcggc ggcgtgatga aggtcggcca
     4201 ggtcgcgtcg ttcatcgacg cgcagttcct gccccccgag ctgcgcgcga tctaccagca
     4261 gcggctcgcg gcgctgcgcg acggcgcccc gccgatgagc tgggcgaggg tccgctgcgt
     4321 gatcgagagc gagctgggcg ctcgggtcga gtcgctgttc gaggagttcg accacgacgc
     4381 cgccgcggcg gcgtcgatcg gccaggtgca ccgcgcggtg ctgccgggcg ggcgcgtggt
     4441 cgcggtgaag gtgcagtacc cggacatcga gcacgcgctc gacgccgacg tgcagaccgc
     4501 ggcgagcctg atcccgctct cgaagccgct gctcccgggc ctcgacgccc agccgctcgt
     4561 ccgcgagctg cgcgagcgcg tgctcgagga gctcgactac gagctcgagg cgcgccacca
     4621 gcggctgttc gccgacgcct accgggggca cccgttcatc cacgtgccgg acgtcgtgga
     4681 cggcctctcg cgccgccgcg tgctcgtgac cgactgggtc gacggtctgg gcttcgagga
     4741 gatgtgcgcg ctcccgcagg ccgagcgcga ccgggtgggc gagatcatcg agcgcttcta
     4801 ctacgtgccg atctaccggc tcgggcgctt caacaccgac ccgcaccccg gcaactacct
     4861 gctgcggccc gacggcaggg tcgcgttcgt cgacttcggc agcgtgcggg ccatcgatcc
     4921 gaggcaggtc cgcgagctgg tcagctcgat cgaggcggcg ctcgacggcg acgcgcggcg
     4981 gctggcgcgc cgcttcgccg ccatgggctt cctcgactgg cccgacgaga tcgacgccga
     5041 cgcgctgctc gagcgcacgc tcgcgtccgg cgactggtac ctgcgcgacc gcgagctgcg
     5101 gatggacccg gactacgtgg cccgctgcgt ggcggcgctg ctcgacccgc gctcggtccg
     5161 cggtggcctc gagctggccc ggcacacgaa cctgccgccc caccacctct ggctgctgcg
     5221 cgtggagctg ggcgtgctgt cggtgctcgg ccacctgcgc gcccgcggca actggcaccg
     5281 ggtcgcgcgc gagctgtggt tctccgagga gtccgagacc gagctgggcc gcgccgagca
     5341 ggcgttcttc agaggtccag caggttcggc tcgccccgcg cgtagcgctc cgcgtcgagc
     5401 tgcttcgccg gctcgaactc cggcggctgc cacagcttga agatcgcgca cgggtcgacc
     5461 tgcgagccgg cgcgcgcgag caggccgttg accgcgcgcc gcgcggactc gttggccgac
     5521 tccatgcacg cgatgtcggt gttggtctgc gggtagtccg aggccaggaa caggttgtgg
     5581 atgccggtga ccgagtccgg ccgcttggcc caggtctcga cggtgttgat caggagcggc
     5641 gtggcgttgc gcgcccgcac gcggtcgccg gggcggtagc cgatcgccgg gtcgaggaac
     5701 caggacttac gcgcgccgtc ggggagggca tcgcggccgg tgtcgttgag atgtcccttg
     5761 atctgcgccc acacctcctc ggctatctgc tccgggcgca gctcgcgcgc gggcttgccg
     5821 tagaggatgc ccggcgtgtg ccagtcggag atcacgaccg agaggcagtc gcgcacccgc
     5881 ccgtcgccgt ccttcgcgaa gtcgtgggtc cagaactgct gctgcgagat cgaggtgagc
     5941 gcccacggcg agtccacgta gctgacgtgc ccgtggagga tcggcagctc gcggtcgagg
     6001 tagaactgga tgccggtcat ccagtcggtc acgagcgagc ccgtgccggc gaggcggggg
     6061 tcggccgcga ggacggcggg gctccagagc ttgcgggcct gctcgaccgg cagcgcgcag
     6121 aagtaccagt ccgcgcgcgc gacctcgtct ccgcgcggac cgcggaggcg gacgccggcg
     6181 atcttgccct tgcgcacgtc gaggcccatc acccgccgcc tcagcttgaa cgtgaccccg
     6241 cgccggcgca gctccgccac ccacgggtcg agccaggcct cgctcgtcgg accgttgagc
     6301 acccggtcgg ctccctggta ctcgccgcgc ggcttcatcg cgttctggta gacgatctgc
     6361 tcggccatca gcccgatcgt gcgcgcgctc gcgctctcgg gcttggcggc cgcgaagttg
     6421 cgcgcgatcc cggctccgaa gaagcgcttg tactcgtcgg agaagcggtc ggcgccgacg
     6481 aagttccacc agctctggcg ctcccactcg cccaggcggc gctcgtcgga gctcgacatg
     6541 aagacggaca gcttgcggcc gaagtaggcg gactcctcgg gcgggacgtt cgagatctga
     6601 gcgaacccgg cgacgaccgc ctgcgcggcc tcctgcggcg tccacgggtg gacggcgagc
     6661 gggatgatga ggtcctcgcg cccgccgttg cgcgcgaagc ccgcctgcga ggcctccacc
     6721 aggttctcga agacgccctc ggcgttcttg ccgaacggga tccggcgcat ggtgtcgggg
     6781 aggtggcggt agaagccggg gaagaagcgg tagccgtgct cgcccgggag gtcgcggcgg
     6841 ccgccggagc ccgagcccgg caccgggatg ctgcgcgcct tgccgcccag cgcgccgtgc
     6901 tcgtagaccg tcacgcggaa gccgcgctcg accagctcgt gcgcggcgct cagcccgccg
     6961 atccccccgc cgaggacggc cacgctgcgg ccgtcgcggc gggcggcgtg tgcacggccc
     7021 gggcgagcga gcgcgcccgc ggcaccggcc gcggcggcgc tgccgagcag gccgcggcgc
     7081 gtgaggctgc gcgtggtgtc ggtccccccc gccacgcgtc catcgtaacc cgcgatcgca
     7141 cgtcgccgcc agtcttgcgc tatgaggtaa cacagcgata atgacccgag tgaacgtctc
     7201 cgggtccttc atcgctccgg ccctcgtccg gcggccggca gccgagggcc tgccggccgg
     7261 cccgtggctg ccggagctga tccagtcggc gcagtacggg ctcgacccgt acgggttctt
     7321 cttccgcgca cacaggcgct tcggcgacgc ctacacggtg cgcgtgctcg gtcagacgtg
     7381 ggtcgtggtc gcgcaccccg actcagtgcg cgagctgttc cagctcggac ccgaggacgt
     7441 gaactcgggc gagacgaacg tggacctgcg cccgatcctc ggcaccggca acgtcggcct
     7501 cctcgacggc gacgagcacc tccagcggcg ccgcatcgtg ctgccctcgc tacagcgcga
     7561 gcggatgccg gcgtacgccg agatggtcgg cgaggtggcg ggccgccgga tcgccgagct
     7621 gcccgtggac acgccggtgt cggtgctgcc gcggatgcgc cggatcacct tcgacgtgct
     7681 gctgcgcggc gtgctcggag ccgaggaccg cgcggcggtg gaccggatcg acgcggaact
     7741 cgaatcgctg ctcgcctact cgatgaaccc ggtccgcggc gcggtgttcg cgctgctcgg
     7801 ccccgaaggg ctcgcccgga tgcccggctt ccgccgccgg cacgcggccc tcgacgagga
     7861 cgtgttcggc gagatccgcc gccgccgcga cgacccgcgg ctcggcgagc gcgaggacct
     7921 gctctcgcgg ctgctgcgga tccgcgacga gcgcggcgtg gcgctgcccg acgaggagct
     7981 gcgcgacgag atcgtgacgc tgctggtcgc gggccaggag accaccgcct cgctgctgag
     8041 ctgggccgtc cacgagctgg cccgcgcgcc cgaggcccag gagcgcctcg ccgccggcga
     8101 ggagggctgg gcggaggccg tgatccgcga gaccctgcgg ctccacccgc cgatcgtgat
     8161 catcctgcgg cggctgcgcc acgagctgcg cgtggccggc cacgacctgc ccacgggcac
     8221 gaccatcgcg atcgccgccc tgctcgtgca ccgccggccg gacctctacc ccgagccgga
     8281 cgcgttccgg cccgagcgct tcctgggcaa gcggaccacg ccgggggact ggatgccgtt
     8341 cggcggcggc gcgcggcgct gcctcggcgc gccgctggcg cagctcgagg cgcggatggt
     8401 gctcgagcag ctcgcgcgcg agcacacgat ccggcccgcc cgcgcacgcc gcgagcgcgc
     8461 cggccggcgc ggcatcgtga ccgtccccgg ccgcggcggg cgcgtcgtcc tctcccggcg
     8521 gagcgcctga cgtgaccgcc cccgcgaacg actccgggct ctcccgccgc cgcttcatcc
     8581 gcgacaccgc cgtcgccggc gccgggctcg cagcgctccc gggcgcctcc gcggcggccg
     8641 cgcgccccaa gcgccgcacc cggcgcacgg tggcgatctt cggcgcgggc gtgggcgggc
     8701 tcaccgccgc gcaggagatg cgcgagcgcg gcttcgacgt ggtcgtctac gagcgcaagg
     8761 cgctcggcgg caagtcgcgc agcaacaccg tgccgggcag cgcgcgcggc ggccgccgtg
     8821 agctgccggg cgagcacggg ctgcgcttcg tgcccggctt ctaccacaac cttcccgaca
     8881 cgatgcggcg catcccggtg gcggggaacg agaacggcgt cgagggcaac ctgatcgcca
     8941 cgcgctcgtc gagcttcgcg cgcgacggcg gccgcgagga cctcgtcttc ccgccggact
     9001 cgcaggaggc gagtggcgag tccctgttcc agtggctgaa cggtggcttc acgcagggct
     9061 cgaacatccc tccgcaggag atcgcgtacc tctcgaaccg gctggcgatc ttcttctcga
     9121 gcagcgacga gcggcggctc ggccagtggg agcacgtcag ttggtgggac tacctcaacg
     9181 ccgaccgccg ctccgaggag tacaggcggc tgctcgtgaa cggcgtgacc cgcaacttcg
     9241 tcgcggccaa gccgcagctc gccagcacgc gcacgatcgg ccggatcttc gaggcgttcg
     9301 cctacacctc gatgcgccgc gacggcggcg gcgagttcga ccgcgtcctg aacgccccga
     9361 cgaacgaggc ctggatcgac ccgtgggtca agcacctgcg caagctcggc gtgcggttcc
     9421 gctcgggcac ggtgcggagc ctcgcgctca agcgcgggcg catcgcctcc gcgtgcgtgc
     9481 gcgactcgaa gggccgcacg cgcacggtcg acgccgactg gttcgtgtgc gcgatgccgg
     9541 gcgaccgcgc gcggagcctg tggacccccg cgctgcgcag ggcggacccg cgcctcgacg
     9601 cgatgcgcac gctccagacc gcctgggcca acgggatcca gttctacctg cgcgacgagg
     9661 tgccggtgct gcacgggcac atcagctacg tcgactcgcc gtgggcgctg acctcgctca
     9721 gccaggcgca gatgtgggag ggccgcgact tcgcccgcga ctacggcgac ggatcggtgc
     9781 gcgactgcct ctcggtgacg ctctcggact gggacgaccc gggcatcctg ttcggccgcc
     9841 cggcgtcccg gtgcacgccc gacgagatct cgcaggaggt ctgggcgcag gtcaaggccc
     9901 atctcgagga caccgggcgc acctacctgc ccgagggagt gctgcactcc taccacctcg
     9961 acccggcgat cagctacaag ccccgccgcc ccggcgacgg cggcgtgcgc gcgcgcaacg
    10021 acgagccgct catgctgaac gcggtcggct cgtggagcag ccgcccggag gcgcgcacgg
    10081 cgatcccgaa cctcttcctc gcggccgact acgtcaaggt cgacatcgac ctcgccacga
    10141 tggagggcgc gaacgaggcg gcgcgcgcgg ccacgaacgc gctgctcgac gcgtccggtt
    10201 cgaacgcgcc gcgcgctgag atcagggagc tgtaccggcc gcccgagttc gaggaggcca
    10261 agcgccagga cgccgagcgg ttcgcgcgcg gcgagccgca cgtgctcgac gtcccggagc
    10321 cgacctagct agacccccgc ctcctcgacc tcctcgagca cgccgtccca gagggcgctg
    10381 cgcgcctcga gggcgcgaac cgcggccacg tccgcctcgg ctcgcagggt ctcgtcgtcg
    10441 ccgcagatcg agttcaggag ctgctgcgcg agcggaccgt gctcgtcgtg gtcgagggtg
    10501 atgtggcggt cgaggtagta cgacagccgc gggatcgagt ggcccccggc cttcagcgac
    10561 gcgacgatcg gctcgaacat gtccgggatc acgtcctccc gggcgtagca gaacgaggcg
    10621 gccacctcgt ggtcggcgcc ctccagcgcc gtctcgagcg tgtgcgtcac gaactcgtcg
    10681 acccggcccg ggacgccggc cgtggacagc gcctcgatcg ggtcctcgcc ggccgccagc
    10741 cgcgcgtggt aggcgtggat cggaccggtg tcggcgccga gctcgcccat cgcctcgagg
    10801 tagagctcga agtggctgcc gtggttgccc tgctcgtcct cgtcggactc ctcggccagc
    10861 acgatgctgt tgatcagccg cgcgtaggtc gggttcgcgg gcggctgcca cggcacgtcc
    10921 acgcacgtca ccgtccgctg gagccgcttc agcaggctga agaagtccca gaccgcgaag
    10981 acgtggtgct tcatgaatat ctgcaccgcc tccacgctgt tcaatcgtcc gtagagctcg
    11041 tggtcgatca gtcgcgcgcg cacgacttcc acgtcggcgc tgtaggtcac cggcatcagg
    11101 ttcccctctc cgtatggata cggccctgcg cgacgtgctg cggacccggt ccgcagggcc
    11161 gcgactttac agaacggaac cgctccgcgg gcgatccgcc cctgtcgacg ggggcctttt
    11221 cacccgttcg gtcgaaccga ctcgtcgcac cgggggtgaa gcgccgcacg cgctgccgga
    11281 cggctgcgcc acgcgagtgc gaggccgctc caccatcggc gtcgaattaa gacacccgcc
    11341 tgggtggata ctccattccg gcccgcgaac cgcgctcccc acggtggaag tgtgcggcgc
    11401 ggcgagcagg cgggcgcccc gcccgatcgc ctctgtgtcc tcgaaaaggg tgggggtgtc
    11461 gcgatgtcta cggtgctgct cttccccggc caagggagcc agcagacagg gatgcgcgac
    11521 ctcgtcgagc gccgccgtcc ggacctgctc gccctcgcgg tcgagctggt gggggccgac
    11581 ccgttcgagc ggatcgacgc cggaaccgcg tacctccagc cggccatcta ctgcacgacc
    11641 atcgcctcgc tgcacgccgt gcgcgcgctc gagcccgatc tctatgccgg ccactcgctc
    11701 ggcgagctgg ccgcgctcgc cgcggccggc agcatctccg aggacgacgg gctgaggctc
    11761 gtcgccgcgc gcgggcgcct gatgcagcgt gcggcggagg ccgaggggga cggcgcgatg
    11821 ctcgcggtcg gcgcggggat cgacgccgcc acggagctcg cgcacgcgta cgggctgacc
    11881 gtggcgaacg acaattcccc cgagcagacg gtgctgtcgg ggcccgccgc ggcggtcacg
    11941 gacgcgcgcg acgacgtcaa ggcccgcggg ctgcgcgcgt tccggctgcc gatcgacggc
    12001 gcgttccact cccccgcgat ggcggcggcg ctgcccgagt tccgcgcgct gctcgacgcc
    12061 acggaggtgc gcccgccgcg gcggccggtc ttctcgtgcg tgaccgcgca ggagttcgac
    12121 gacgtgcgcg cccgcctggc ggagtcgctc gtccgcggcg tgcgctggcg cgaggtgctg
    12181 ctggcgctcc acgcccgcgg cgccgaccgc ttcgtggagg tcgggcccgg ccaggtgctg
    12241 acgaagctcg cgcgggccac cctccccggc gtcgacgcgc tgccggccga ggagctggag
    12301 gcggtcggtg cctgacgccg cggccggcgc ggtcgccgag ccgctgcgcc cgccgcgcgc
    12361 gacccgcggc gcgggcatcg cggcgctcgg cgtggccctg ccgcgcacgg cggtgcccaa
    12421 cgccccgata gcggagcggc tgggtgtgga cgaccactgg atcgtcaagc gcatcggggt
    12481 gcacgagcgg cggatggtgc aggacgacga gcggctgagc gacctggtcg agcgcgcgtc
    12541 gcgtcaggcg ctcgagcggg cgggggtcga gccggaccag ctcgacctga tcctggtcgg
    12601 cacgttcacc caggacgaga tcctgccgaa cgctgcgccg ctcaccgcgg gcgcgctcgg
    12661 cgccacgcgc gcgggcgcgg tggacctggg cgcggcctgc atgggcttcc tctcgggcgt
    12721 cgcgctggcg gctgcgcaga tcgaggccgg ccgcgccgac acggtgctcg tggtgggcgc
    12781 cgaggtgctc actcgcttcc tcgacatgga cgaccgccgc accgccgcgc tgatcggcga
    12841 cggcgccggc gcggtggtga tgaccgcgat cgacgggccg agccggatcg gcccgtgcat
    12901 cctgcgcgcc gacgaccgct cgcgcgaggt cgtctacatg acccgcgagg acggcaagct
    12961 gcgcatggag ggccagcaga ccttcgcgat cgcggtcgac tcgctgtcgg acgtgagccg
    13021 gcaggccgtg gcggcggccg gcatgacgct cgacgacatc gacctgttcg tctaccacca
    13081 ggcgaacgcg cgcatcacga aggcggtcgg cgagcgcctc gggctgcgcg gcgagcgcgt
    13141 cgtggactgc atcgaggcgc ttggcaacac gtcggcggcg tcgatcccgc tggcgctggg
    13201 cgtcgccgcg ggcgaggggc gcctgcacga cggcgacagg atcctggtgg ccgccatcgg
    13261 cgccgggttc acctggggcg cggctgtcat cgagtggagc ggccgtgccg tctgacggcg
    13321 gctgcgccct ggtcaccggc gcctcgcgcg ggatcggcgc ggccaccgcg cgcgtgctcg
    13381 ccggcgaggg ctggcccgtg ggcgtgaact accgcaccga cgaggacggc gctcgcgcgg
    13441 tggtcgagga gatcgagcgc gagggcggcg aggccgtggt cgcgcaggcg gacgtgtcgc
    13501 agtccgccgc ggtggacgag gtcttccgcg cgctcgagga gcgctacgag cgcgtggccg
    13561 tgctcgtgaa caacgccggc acgcgcgccg acgggctctc cgtgcagctc gacgacgagc
    13621 agtgggacac cgtgatgagc acgaacctgt cggccgcgtt ctggacctgc cggcgcgcgc
    13681 tgatgccgat ggtgcgctcg cgcttcggcc ggatcgtcaa catcgcgtcg gtcgtaggcc
    13741 cgtgcgtggc gaaccccggt caggccaact acgccgcggc caaggctggg ctggtcggcc
    13801 tcacccgcac ggtcgcgcgc gaggtcgcgc accgcggcgt gaccgtcaac gcggtggcgc
    13861 ccggcttcgt cgagacccag ctcaccggcg acgtgagccc cgacttcatc aaagccgtac
    13921 cggcgcgccg cgccggcaag cccgaggagg tcgcggcgtg tgtccgcttc ctcgcatcac
    13981 gcgacgcgac ctacgtgacc ggcgcgttgc tcaccgtgga cggcgggatg accgcctagc
    14041 cgcccgaccg aaggaggacc cgtgccatca accgaaaccg acccgcagat cgtggaggaa
    14101 gcgatcatcg agtcgatcgc gtacctgggg gccccgcgcg ccgatatcaa gcgcgaggcg
    14161 acgttcgaag agctggacct cgactcgctc gacctggtcg agatcgcgca gatggccgag
    14221 gagaagtggg gtgcgagcct cgagccccag gacctcggcg gcgtgaagac ggtgggcgag
    14281 gcgatcgacc tggtgattgc ccggctgccg tgagccggag ggtcgtgatc accggcgtcg
    14341 gggcggtcac accgctcggc gtcggcgcac gcaccctgct gcgccgctgg gcggaggggc
    14401 agagcgggct cgagggcggc ctcggccgct gcgacgagtt cgaccccgag cagtcgctgt
    14461 cgcgcaagga ggcccatcgc agcgaccgct tcacccagct cgcgctgacc gcgtgtgcgg
    14521 aggcgctcgc ggacgccggc tgggacgacg gccccccgtg cccgcccgag cgcgccggct
    14581 gcgtgatggg caccgggatc ggcggcatcg ccacgatcga ggtccagcac gacacgctcc
    14641 gcgagcgcgg ccagaagtcg ctgtccccgt tcggcgtccc gatgatgatg ggcaacgcgg
    14701 ccgcgggcgc gctcgcgatg cgccacggcc tgaccgggcc gaactggggc gtcgtctccg
    14761 cctgcgcggc cggcgcgcac gcggttggga gcgcggtgcg gctcgtgcag agcggcgagg
    14821 ccgacgcggt ggtgacgggc gggaccgagg cggcactcac gccggtggcg atggggctgt
    14881 tcgcgcggat gggagcgacc tcgccgaccg gggtctcacg acccttcgac gcgcgccgcg
    14941 acggcttcgt gctcggcgag ggcgccggcg tgctcgtgct ggaggaggcc gaggcggcac
    15001 gcgcgcgtgg ggccgcaacg ctcggcgagg tgctgggcta cggcgccacc gccgacgcct
    15061 accacctgac cgctccggac ccggccgggg acggcgcggc gcgcgcgatc cgggcggcgc
    15121 tggacgacgc ggggatcgag ccggaggcgc tcgactacgt gaacgcgcac ggcacctcca
    15181 ccccgctcaa cgaccgctcg gagacggccg ccctcaaggc ggcgctgggc gaccacgccc
    15241 ggcgcgtgcc ggtctcgtcg ctcaagtcgg ccatcgggca cctgtgtgga gcgggcggcg
    15301 cggtcgaggc gatcgccacg gtgctggcgc tgcgcgagcg tgtggctccg ccgacgctga
    15361 actggagcga gccggaggag ggcctcgacc tcgactacgt gcccgagcac gcgcggccgc
    15421 tgcgcgacgg cgccaacggc ggtcgcgccg tggccatctc gaactccttc ggcttcggcg
    15481 ggcacaacgc ggtgctgtgc ctggcggcgg actagccgtg gcggccggga ccgagaccgc
    15541 gcgcgccgcc ctcgggtgct acttcgacga gctcgaggcc gggcagggct tcggctcgcg
    15601 cgggcggacg atcaccgagg cggacatcgt cgcgttctcc gcgctgacgg gcgactggca
    15661 cccgcagcac tccgacgccg tctgggcggc ggcgagcggc ttcggcgagc ggatcgccca
    15721 cggcatgctg gtcctgtcgt tcgcgctcgg cctggtcgag ctcgatcccg accgcgtcgt
    15781 cgcgctgcgg cgcgtgcgga cggcgacgtt caaggcgccg gtggcgatcg gcgacacgat
    15841 ccgcgtcgag ggccgcctcg gcggcatgcg tgaggtcgac ggcgagcgcg gactggtcga
    15901 gacgagcctc agggtggtca accagcacgg gcgcgcggtc gcgtcgttct cgctcgacct
    15961 ggtctggcgc cggtccgagg cccgtcctcg agaggcgaag gcgatcactt gagggccgcc
    16021 gcgccacgcg cgacgtcgcc ggcgcccggt cccctggcga tcgtgggcgg ctacggcctc
    16081 gtgggagcgc aggcggcgcg gctgctccgc gagcgccacc cggacctgcc gctcgtgctc
    16141 gcggggcgcc gtccggcgac ggcgcgtgag ctggccgcct cgctgcgcgc gaccacggcg
    16201 agggtcgatg tcggcgagga gcgcccgctg cggtcgctgc cccaccgccc tgcggccgtg
    16261 ctcgccgccg tgtccgaccc gcacgaccac ctgctcgccg acgcgatgcg cgcgggcatc
    16321 ccgatcgccg acatcaaccg cggcggggcg gcagcggtgc tcgacgcgtt cgtgcgcgcc
    16381 acgaccgagc gcccgcgcgc gccggtgctc ctgtcgggga gctggctgtc ggggctcacc
    16441 gcgctggtcg cggcggcggc cgtccgcgag atcggcggcg cggagcgcgt cgaggtggcc
    16501 gtgctcgtct cctccggcga ccgcgtcggc gacgacgcct ggggcttcag caagcgcctc
    16561 gcgtttccct accacgcgat ggccggcggc aggcggcagg cggtccagcc gctcacgggt
    16621 gtgcggcacg tgcgctgcgc ggacgggcgc gagcgccccg ccgcgcgcgt gggcacgctc
    16681 gagcagatca ccctgccgat cgcgctcggc gtgccgacgg tcgagacgcg catcgcaccg
    16741 cacgacgccg ccgcgctgtg ggcgctcgtc gggctcaagc ggtcgggcgc gctgcggacg
    16801 ctcgagctgc cggcgctgcg gcgcgtgcga gcggcgctgc tgcacaggtc ggggcccggc
    16861 gacttcgccg gcttcggcgt gaccgctcac ggggacgggt gccacacgac catcgaggtg
    16921 ctcgacgtgc gcgggcagtc gcacctgaac gccgtcggag cggcgcgcgc cgccgagcgc
    16981 gtgctcgggc tcgccggcgc cgagctgacg ccgggcgtct cgttccccga gcagtccgcc
    17041 gatcccccgg ccgacctcgc cgcgctgagc gaggccgggg tggtggtgcg ccgcggcggg
    17101 ttccgccggc ccgcgcccgc cccgggcccg ccggccaccg acgcagacct cgtgttcatg
    17161 cccacggaga tcccagagga gaacctgacc agatgagctc cagacgcgcg cgtgcgccac
    17221 gcctgaccct gtccatcctc cgcctcgtgg gcggcgcggc gttcctgctg ccacgcgtgg
    17281 gcgtgaagca gctcggcctg cgcgacgacg ccgagagcgc ctacctcatg cgcctgttcg
    17341 cggcccgcaa cgtcgcgatg acgaccggcc tgctcctcag ccgcggcgac gcacgccggc
    17401 tgtggtggca ggtggggatc gtgtgcgacg tgctcgacgc gggcgcggga ctgctcggca
    17461 tgcgggaggg caaggagcgc acgtcggccg tcgtggacac gggcgcgtcc ctggtggcca
    17521 ccggtctcgg cgtggccggg ctgatcgcgg acaggggctg agggggagcg atgaccacga
    17581 tcgagcgaac accgagccgc agcgacgacg gagcgagcgc gcgcgcggag cgggtgtgcg
    17641 tggtcggggc aggctcggcc gggatcgcga tgtgccgcgc gctggccgtt cgcgggatcc
    17701 cgttcgactg ctacgagcga accgacgcgg tcggcggcct gtggcgctac ccgcccgcgg
    17761 gcggcggctc gtgcgcgtac gcctcgctct acccgaacac ctcgaagacc gtcatgcagt
    17821 tcccgagcta cccgatgccg gaggactacc cgcactaccc gcaccacacg gcggtcgcgc
    17881 gctacttcga cgactacgtg gaccacttcg gcttccggga cgcgatccgg ttcgggaccg
    17941 aggtcacgtc ggtcgctccg gcggcgggcg gcggctggga cgtgacgctg ggcgacggga
    18001 ccacccaccc ctaccgggcg gtcgccgtcg cgtcgggcgg ccggcacgcg gagccggtgt
    18061 acgccgagct ggagggcgag ttcgaggggc gccagctcca ctcgttcgac tacgacggcc
    18121 ccgccgactt cagggacagg cgcgtggtgg tgctcgggct gggcgcgacg tcggcggaca
    18181 tcgcgtcgga gctgtcgctc gtcgcccgcg cgacatatct gtcggtgcgc accgggcact
    18241 acgtcgtgcc gaagctcatc gagggccgcc cgatcgacgt gctgtcgccg ctgatgaagc
    18301 ggctgtcggt cgagcagcgc cggccgcttc tcaccttcct tctgaagttc gtgaacggga
    18361 gccctacaga cttcgggctg ccgaagccgc cgtacaagcc ggggcagggc ccgctgatcg
    18421 cgaccaccga cctgctgccc gcgatcgcgc acgggcgcgt ggtgccgaag cccgtcgtcg
    18481 cgacggtggc gggatcgacc gtgcgcttcg ccgacgggtc cgaggtggag gcggacgcga
    18541 tcgtgcactg caccggctac cgcatcggct tcccgttcct cgaccgctcg ctcgtctccg
    18601 gcggcgacga cgccccgccg ctctaccggc tggtcgtggc gcccgagctc gacggcctct
    18661 acttcatcgg gctgctccac tcgatgatgt cgctgatgcc gctcgccgag gcccagtccg
    18721 agtgggtggc cgaggtgctc gaggggagcg tcgccctgcc gccgcgcgag cggatgtggt
    18781 cggagatccg gcgcgagcgc cggcgccagg atcgccgctt ccacgactcc tccggccacc
    18841 tgctggtcga ccccaacgaa tacgagcgcc tgctcgcgcg cgagcgggcc aagcacggag
    18901 cgcccgcgcg cttatgatcg ggcctcaccg gcaacgcccg aggggagctc gaggatgaag
    18961 aacgaggaac agcagccgtc acggggaggc cgtcccggcg gcctggatct atcaaggctg
    19021 agccaggccg agctcgccga gctcaaggag gagatcatcc gccgccagcg gcccgcgggc
    19081 tcgcgcctcg agccgacgat ggcgacgacc gacgacggct cgttcttctt caccgacgac
    19141 cggctggcgt gggacgacag gtgggtcacc gtcatcgacg cgatcagcca gggcgtgaca
    19201 cgcatcgccg ccgcgcagat cgccgccggc agcgcgatgg cctcgtcgca gatgatgcgc
    19261 gcggggaacg accgcatggc caaggccacc ctcggtgtcg cggccgctgc cgcggccggt
    19321 ctcgccggta ccgccttgcg ggacgcacgc cgccagcgct gatcccgcgc aggcctggta
    19381 ttcgcaggcc cgacatcagc ttctgcatga tcgcgcgcga cgcggccgcg acgctgcccg
    19441 catgcctggc gagcgtcgcg gccgtcgccg acgagatggt cgttgtcgac accggctcga
    19501 gcgacgacac ggcggcggtc gcgcgcgagc acggcgcccg cgtgatcccg ttccggtggt
    19561 gcgacgactt cgccgccgcg cgcaacgcgt acgtgcgggc ggcgcgcggc tcgtgggtgc
    19621 tgtcgctcga cgccgacgag gtgctcgacc cggtcgaccg ggaggggttg cgggccgtcc
    19681 tcgcggcgaa cccgcgaacc gcgtgcgtcg tcacggtccg caactacttc gcgctgggcg
    19741 agctgaactg gccgtacatg ccgagcgaat tcctcggcga ggtgagcccc ggcatcgggt
    19801 gcatgacgag ccggacgatc cggctcttcc cgcgggccac gctgcccgag tatcgcttcc
    19861 ccgtgcacga gtcgctcgtg ccctcgctga ggcaccgcgg cgtggcgata cgccactgcc
    19921 ggctcggcat ccaccacacc ggctacctcg gcgggcgcgg cgagttcgag gcgcgcgcgg
    19981 ccctctacgg gcggctcggg gagcggaagc tcgagctctt ccctcgccac ccgctcgcgc
    20041 acctcgagct cgggcgggtg cggctgttcg agggcgagat cgacgacgcc gcgcggctgt
    20101 tccgcaactg catccggctg cgcccccggc tcgtgcccgc gcacttcttc tacgtgctcg
    20161 cgctggcgca cggcgggcat tccggcgaag cgcacggtca cctggcgcgg gcgctggagc
    20221 tgatgcccgg ggatccggac ctcctgtacc tgcgcgggct gctcgccctc gagtcggccg
    20281 ggcccgaggc ggtcgccgcg gacctggcgc cggcgctgcg gcggatgtcc gccggcgcgc
    20341 cggagcgcgc tcagatgccg ccgcgggtgc tggcgagcgc gagttcgcgc agcgacgcgc
    20401 cgtagaaccc gcgcgcggcc agggcgtcga gcagcgtgac caggtgctcc tgcgcgctcc
    20461 tgcggcgcag gcagcgcggg tggagcacga tcccggcgtg cccgtggcgg gcgcggtcgg
    20521 cctcgatcgc ttcgaggatc gcctggtggg ggcgcgggcg tgcgcggtcc cagtcccaga
    20581 tgtcggtccc cgcgcgcacg accgggatcg gcacgggcgg cagcgaccgg tcgaagaggt
    20641 gcgacacgaa caggccgccc gcgccgtgcc agacgtcggc cagccacaac ggcagcgcgt
    20701 cgaacggcgg cgagaggccg ccgccgaagc ggtccgggaa ggcggcgagc agctcggccc
    20761 gcccgcggcg gatcaggcgc acctcgtcgg gcgccggccg ctccgagtcc ggggcgaact
    20821 cgtgacggcg cccggcggca tccgagtgcg ggacgtgcgc gtagccgtgc tgtgcgacct
    20881 cgaacaggtc gccgccgggg tcgaacgggg caaggctcgc ctcgttcagc tcgttgaggt
    20941 aggggaccac gtcgagcgag gcgcgcagcc gccgctcggc gaggatgcgc aggagccagc
    21001 cgagggtcag ctcgggtacg gcgacgtcgt cgacgcggat cgcgagcggc gacccggcgc
    21061 gcgcgggccg gcgccggcgt ggggtccgca gcgcagacct tccgcgaacc ggcgccgggc
    21121 gcgggggccg cacgcgcacg cggcggcgct ctcccacgag gagggtgcgc agccgggcgg
    21181 cgtccggcct gccggggttg cgcgcggtga tcgcccggca gcgctcgcgg aagctcgcgc
    21241 gccagtcctc gtcccaccag agccgctgcg ggtcgtcgca caggaggccc tcggcgcgca
    21301 gccgcgcgag ccgctcgtcg ttcggctccg cgaacgtgct gcccacgaac ggcaggaacg
    21361 ggacgccccc cgcgacgcac tcccagatcg tgttgaaccc cgacctgacc accgccgcgt
    21421 gcgcgaggct cagcagcgcc ggcatgcgcg gctcctggtc gacgacctcc aggcgcgggt
    21481 cgagctccac cccgtccgga aagtaggggc cgcgcacgaa catcagccgc gcggacgggt
    21541 cgacggcgag tatcgcgtcc gccacctcgc cggcgagccg cgcgaactcg accgcgtcgg
    21601 gatccgtcga gcggtgaagc ccgccgcccc ccatgctgaa cacgcacagc cggcgctcgc
    21661 cgtcgatccc gtggcggcgg cgcacctcgt cggcgtcggc ctccgaggcg cgcctgtaga
    21721 cgggcccgac ctgaccccac tgcggccact gcgacaggac gcgcgtctcg ctctcggagt
    21781 acggccacgt cggcgagccg gcggcgtcgc agagcacgaa gctcgagtag acggaggagt
    21841 agtggtcgtt caggtcctgg aggtacgcga acgtcgtcgg ccgcaccacg agggcgcgcc
    21901 ggatctcctg cggcagcgtg agcgggttcg ggtgggtgtc ctcgatcagc acggccggga
    21961 gcgagagcgc ggccatcgag gcgatctcac ccgacacgcg ccggcgcacc ggctcgtcgg
    22021 tcccccacag cgacggtacg accttgcccg ggtgctccga gcgctcgtcc agcgggaaga
    22081 tgccctgcga gaagaccacc ggccgctcgc cggtctgcgc cagcgcgtcg cacaccgtgg
    22141 tcgcgcgcac gagatgtccg agcccgacgc cgttgagcgc caggaagacg accgccatcc
    22201 tcagccgcgc cggtcggcgc ggtcgtgcag ccgccgctgc tcgcggtcgg tgctccaggc
    22261 ccagaagccg acgagcgagc ccacgccagc cacccgcagc cgatccccgc gcgccacgcg
    22321 ctcctcgcgc agcgccctgc gcgcgtgcga ccagaccgac tcggtcagcg tgagcgcctc
    22381 ggcgtcctcg ccgagcttgc ggtgcatcac gagccgcccg cgcgcgagcc gctcggcctt
    22441 ctgccgcagc tcgtcgagcg tggtcatgca cggatgctcg acggccgccg ccgcctcgta
    22501 ggtgatcggc aggcccgccc gccgcgcccg cagcgcccac tcccagtcct cgaaggccgc
    22561 ctcctcgaag ctcgcgtcga acggaccgac gcgctcgaag gtccgccggt gaaccagcag
    22621 gttcgccgtc acgaacgcgc cgggcccgtt cacgtagcgc tcctgctgga ggtaggtgac
    22681 gctgtcgtag aggctgaccg ggttgcgccc ggcatcgggc cgcacgatcg cccccgcgac
    22741 gatgccgccg ggcgcgccgg tccgcatcgt ggccagcccg gacgcgagcc agccggggcg
    22801 cgccacgcag tcgctgtcga cgaaggccag cacgtcgccg ctcgaggcac gcaccccggc
    22861 gttgcgcgcg gcggccggcc ccctgcgcgg ctcgtgcacg accgacacgc ccggcaacgt
    22921 ccgctccgcg ggcgggtccg cgccgttcat caccacgact atctcgaccg gctgctcgac
    22981 ccgctgcgcc tggagcgact cgatgcaggc tcggagcgga tcgctccagc tcgtcgccgg
    23041 aacgatcacg ctggccgacg cgacgggcag cccgcgcacc ctggacgcgc gggtggcgga
    23101 cggcgactcg agcagcgggc gcaggcaggc gctcgccgcc gtggccgaca ggtgacgctc
    23161 gccgaacgcg aaggccgccc gcccgtgggc ggccgcggag gcgggggcga gcagcagccc
    23221 ggccaccgcg tcggcgcacc ccgccacgga ctccgcctcg gcccaggcga gcgtcgcgcg
    23281 cggcaggccc tcggaagcga gcggcgtggc cacggtcggc ctcccgagcc gcagcgcctc
    23341 gagcagcttg accttcaccc cgcttcccac cgcgacgggg cagacgaccg cggcggcccg
    23401 cgcgtactcg cccgcgaggt cgtccacgtc gcccggcgcg tcgactccct cgagcccggc
    23461 gagaccctcc cccacggcac cgatcacgcg caaccgcgcg tccggcaccc ggctcagcac
    23521 gcgcggccat ccgacccgcg cgaactcgcg gaccatctcg ttgcccggct gtgcggaccc
    23581 gaccgcgagc acggtgcggc cgcccgccgc ggccggcctg tagccgtcgg gcagcggtat
    23641 ctcgggcagc agcgtgacca cctccctgtg cgggaggagc gacccgagga gttcctcgtc
    23701 gcggtgctga gagacgatca gcacgtcggc ggtgcggagc cgctcgacct cctcctcgag
    23761 cgtgcagacc gtccacgggt cgagcccggc ggcctggaac cgggcgcagc gctcgtgcag
    23821 cacgtcgtgg cagtcgaccc agcgccgcgt gccgcgcgga agcccggata gcgcgggggc
    23881 aagccacgcg tactcggcga tcgccacctc gggctcgaac cgccgcgcga cccgatccac
    23941 agcgcgccgg aacgggcgcg ggtcgaacgc cgcgatcgga ccgccctgga acggccgcgc
    24001 gcgcaccgcg atcacgccat ccacgagcgg ccggacgtcg tgcagcgacg gatcctgggc
    24061 gccgatcacc accacgcgaa ggccctgcgc gcgcagcgcg cgcaccacgc cgaggatccg
    24121 cacgcggttc ccggtccacg ccggcatcag acgccggtcg gtgacgagca gggcgacccg
    24181 cgggcggctc acccgaccgc gctccagctg ccggtccggg tcgactcgag cagcgcgccg
    24241 agcacgcgct gcgccgcgac cccgtcggcg aacgtgggga actcgacgcg ctccccgcgc
    24301 agcgcctcga cgatcgcgcc gggcgaggcg ggcgcgccgg acagcgcgag gtccgccggc
    24361 agggcccgcg ccgggatgtc ggtggtccag gtcgcgcggc gctccagcgc ctcacccgac
    24421 agcaggcgga tctcgccgtc gcgctcgctg tccacgatca gcgtcccgcc gtcgcccgag
    24481 acctccagcc ggaacgaatc gccgtagccc gccaccacct gcgaggtctc gaacgtg
//
LOCUS       DASVPL010000050         6153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_201376, whole genome shotgun sequence.
ACCESSION   DASVPL010000050 DASVPL010000000
VERSION     DASVPL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201376"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VF517_02795"
     CDS             complement(<1..193)
                     /locus_tag="VF517_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8051891.1"
                     /translation="MEAAGAAAPAGRGGPAERRLDSDKARRIVAAMRASVARRGAAGS
                     TFDHVAREAGVSRGLLHYYF"
     gene            269..1471
                     /locus_tag="VF517_02800"
     CDS             269..1471
                     /locus_tag="VF517_02800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8051892.1"
                     /translation="MARRKPLQLTLAAVTLAAGFQAASTLSSSQAQTARPAAASAEAF
                     ARQDGRGTHSRVVARGNGTSARGGASVSASTRGGKGRASASAGVAHVNLFDGLVRASS
                     VRVAASSGPGGRRAGRVAGLRIAGAKRGTVTRRRGYDLDGYGRLVVLAAGRHGITGMR
                     ATLTRKYRGTPRGTTVTIAFASASARDAVRPPKPTEPDRGGSGGRKGGGHKRSKEPPR
                     RKAPRTPESRLRAISTKGGFAFPVKMGEHHYSDDWGAARQNTGHHEGNDVFAPAGTPV
                     LAVTDGTLRRVGTARVPGNRLYLYSDSGDYYFYGHLSSFESDAREGAEVKAGQVLGFV
                     GSTGDAEPTPPHVHFEIHPGGGGPVNPYPFLRAWEKKRDVPTAAWLGRYAGARPGTLV
                     VVRDYLAQ"
     gene            complement(1553..2032)
                     /locus_tag="VF517_02805"
     CDS             complement(1553..2032)
                     /locus_tag="VF517_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051893.1"
                     /translation="MASKKKKAASAGAGALAAGKAAKDNPYVQRLVEDDELRDNLRTA
                     YDSARNAYERIANGKGPAKAILDDKKTQKELREAATSLRDAADSLRGSKKKKKGHKGR
                     NLLVLLIAGGAAMALSEGLRKKVLDALFGAEEEFEYTSTTTSSSSGSSAGQPAGAPS"
     gene            complement(2121..4622)
                     /gene="lon"
                     /locus_tag="VF517_02810"
     CDS             complement(2121..4622)
                     /gene="lon"
                     /locus_tag="VF517_02810"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX8051894.1"
                     /translation="MSIHIAHPGSGEANGDGALPDGLPVLPLRDTVAYPHTLTPLAIG
                     QERSIQLVNDVLGRERMLAMVTARDPDLDEPGPDDLYDVGVAGTVARMIKVPDGTLRI
                     LVQGGPRVKLNGFAAEKPYLVARIEEMPDVVEETPELEALVRNVQQTFGQIIQQVPYL
                     PEELQVAVANVDDPSELSWMIAGALRVKPEERQELLAEASLAKRLRRLSELLAREAEL
                     ISIGSRIQSQVQSEMEKGQREYFLRQQLKAIQEELGEVDEQQAEARELREQIDAAELP
                     EHAKKQAERELERFERLPPQSAEHGVIRTYLEWIVSLPWSKSTEDHLDLKEARSVLDR
                     DHYDIDRIKDRIIEFLAVRKLKPDARSAILCFVGPPGVGKTSLGRSIAAAMGREFERI
                     SVGGVRDESEIRGHRRTYIGAIPGTIMRALRDAGSNNPVLMIDEIDKMGSDFRGDPSS
                     AMLEVLDPEQNGTFRDHYLDLPFDLSNVMFITTANQLEPIPPPLRDRMEVIQLAGYTA
                     EEKLEIAKRYLVPRQIERNGLTPGKIEFTEPALELVIDGYTREAGVRNLEREVGSLAR
                     KVAREYAEGTRRRKRIIRPKQVGELLGKRRFLSEVARRTDEPGVATGLAWTPVGGDVL
                     FVEATSFPGDGKLQITGQLGDVMKESAAAALSYVKATAWRWGAGVPDDWFRTNDVHVH
                     VPAGAVPKDGPSAGITMVTALVSLVTGRPVMSDTAMTGEVTLTGQVLPIGGLKEKSLA
                     AQRAGIERVIAPKLNEADTDDFPAHLLDDIEFVFVDSVDRVLDAALEPQRDADGNAVK
                     LPRRRRSSRAAPAAAARADAFLGGTVAGSLRAPPE"
     gene            complement(4619..5077)
                     /locus_tag="VF517_02815"
     CDS             complement(4619..5077)
                     /locus_tag="VF517_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX8051895.1"
                     /translation="MPPERDLFANFERMRREIDELFGDVFERARLGPGRRGFSPPVDV
                     YYCGGRSPRAVVKADLAGIDIEDVGLEIRGRQLVIAGERKPQDAEGRLYQQIEIAHGP
                     FRRVVELGTDVIAEEASATYDDGILRVEMPIATEDPRQVPIRSREPGEPA"
     gene            complement(5101..5895)
                     /locus_tag="VF517_02820"
     CDS             complement(5101..5895)
                     /locus_tag="VF517_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8051896.1"
                     /translation="MRLTVLGKSPSWQDAGGACSGYLVEEDGTTVVVDCGNGVFGKLR
                     KYRDYTRVDAVVISHIHADHWLDLVPFSYALTYAPRQQPVPVDRWPGTEAPARPRLIV
                     PRGALDCFRRVVGVWGNEDLIEKAFTIEEYGEDDTPRVGSLELSFQSVPHFTETFAIS
                     VESTNGGGKLAFGADSRPSVDLVSFARGSDLLLVEATLPRPERSGTRGHLTPREAGEH
                     GKAAGARRLVLTHISDELDALWARNEAEDAFGDRVDLAREGAVYTL"
BASE COUNT          812 a         2244 c         2195 g          902 t
ORIGIN      
        1 cgaagtagta gtgcagcagg ccgcgtgaga ccccggcctc gcgcgcgacg tggtcgaagg
       61 tcgagccggc ggcgccgcgg cgcgcgacgc tcgcgcgcat cgccgccacg atgcggcgcg
      121 ccttgtcgct gtcgagccgg cgctccgcgg gcccgcctcg gcccgccggc gctgctgccc
      181 ctgcggcttc catcgctcgt aatctagctg gacggccagc cagcaggggg ccggcgcgcc
      241 gcctgcggct ctgctacacg atatccccgt ggcccgccga aagcccctgc aactgacgct
      301 cgccgccgtc accctcgcgg cgggcttcca ggccgcgtcc acgctcagct cctcgcaggc
      361 gcagacggcc cgcccagccg cagcctccgc cgaggcattc gcgcgccagg acggccgagg
      421 cacgcacagc cgcgtggtcg cgcgcgggaa cggcacgagc gcacgcggcg gggcctcggt
      481 cagcgcctcc acccgcggcg ggaagggccg cgccagcgct agcgcgggcg tcgcgcacgt
      541 gaacctgttc gatgggctcg tgcgcgcgtc gagcgtgcgc gtggccgcct catcgggacc
      601 gggcgggcgg cgcgcgggcc gtgttgccgg cctgcggatc gcgggcgcga agcgcggcac
      661 cgtcacgcgc cggcgcggtt acgacctcga cggctacggc cgcctcgtcg tgctcgccgc
      721 aggtcgccac gggatcaccg gcatgcgcgc gacgctgacg cgcaagtaca ggggcacccc
      781 gcgcggcacc acggtcacga tcgccttcgc ctccgcgagc gcgcgcgacg ccgtgcgccc
      841 gccgaagccc accgagcccg accgcggcgg cagcggcggc cgcaagggcg gtggccacaa
      901 gcgctcgaag gagccgccgc gccgcaaggc gccccggacg ccggagagcc ggctgcgcgc
      961 gatcagcacc aagggcggct tcgcgttccc ggtgaagatg ggcgagcacc actacagcga
     1021 cgactggggc gccgcgcgcc agaacacagg ccaccacgag ggcaacgacg tcttcgcgcc
     1081 ggccggcacc cccgtgctcg cggtcaccga cgggacgctg cgccgtgtcg gcaccgcgcg
     1141 cgtgccgggc aaccgcctct acctctactc cgacagtggc gactactact tctacggcca
     1201 cctctcgtcg ttcgagagcg acgcgcgcga gggcgccgag gtcaaggcgg ggcaggtgct
     1261 ggggttcgtg ggcagcacgg gcgacgcgga gccgaccccg ccccacgtcc acttcgagat
     1321 ccacccgggc ggcggcgggc cggtgaaccc gtacccgttc ctgcgcgcgt gggagaagaa
     1381 gcgcgacgtg ccgaccgccg cctggctcgg ccgctacgcc ggcgcccggc cgggcacgct
     1441 cgtggtggtg cgggactacc tggcccaata aatccgcgcc aggaacccct gaacgcggaa
     1501 gaggcgcccg ggggcgcctc ttcgcagggt tccgtctaag tccgcggctg gttcagcttg
     1561 gggcgccggc cggctggccc gcgctcgacc cggagctcga ggacgtcgtg gtcgacgtgt
     1621 actcgaactc ctcctcggcg ccgaacagag cgtcgaggac cttcttgcgg aggccctccg
     1681 acagcgccat cgcggccccg ccggcgatca ggagcaccag caggttgcgg cccttgtgcc
     1741 ccttcttctt cttcttcgag ccgcggagcg agtcggcggc gtcgcgcagc gacgtcgcgg
     1801 cctcgcgaag ctccttctgc gtcttcttgt cgtccaggat cgccttcgcg gggcccttcc
     1861 cgttggcgat ccgctcgtag gcgttgcgcg cggagtcgta cgccgtgcgc aggttgtcgc
     1921 gcagctcatc gtcctcgacg aggcgctgca cgtaggggtt gtccttcgcc gccttaccgg
     1981 ccgcgagagc acccgcgccg gccgatgcgg ccttcttctt cttcgatgcc atgactctcc
     2041 tcggtcggct ccttacccgg aacatacccg agtagttgat tcaagacacc ggagccgcaa
     2101 tcggcggcgc gtgcagaccc ttactcgggg ggcgctctga gggagcctgc gaccgtaccc
     2161 ccgaggaagg cgtctgcacg cgccgccgcc gcgggggccg cgcgcgagct ccttcggcgt
     2221 cggggaagct tgaccgcgtt gccatccgca tcacgctgcg gctcgagggc cgcgtcgagc
     2281 acccggtcga ccgagtccac gaacacgaac tcgatgtcgt cgagcaggtg ggcggggaag
     2341 tcgtcggtgt cggcctcgtt cagcttgggc gcgatcacgc gctcgatccc ggcacgctgg
     2401 gcggccagcg acttctcctt caggccgccg atcggcagca cctggccggt gagcgtgacc
     2461 tccccggtca tcgccgtgtc ggacatgacc ggccgcccgg tcacgagcga gacgagcgcg
     2521 gtgaccatcg tgatcccggc gctcggcccg tccttcggca cggcgcccgc cggcacgtgc
     2581 acgtgcacgt cgttcgtgcg gaaccagtcg tcgggtacgc cggcgcccca gcgccaggcc
     2641 gtcgccttca cgtacgagag cgcggccgcg gcggactcct tcatcacgtc gccgagctgg
     2701 ccggtgatct ggagcttgcc gtcgccgggg aacgacgtgg cctccacgaa cagcacgtcg
     2761 ccgccgaccg gcgtccaggc gagcccggtg gccacgcccg gctcgtcggt ccggcgcgcg
     2821 acctccgaga ggaagcgacg cttgccgagc agctcgccga cctgcttcgg ccggatgatc
     2881 cgcttgcgcc tgcgcgtgcc ctccgcgtat tcgcgcgcca ccttgcgggc gagcgacccg
     2941 acctcgcgct cgaggttgcg cacgccggcc tcgcgcgtgt agccgtcgat cacgagctcg
     3001 agcgccggct cggtgaactc gatcttccct ggcgtgagcc cgttgcgctc gatctggcgc
     3061 ggcaccaggt agcgcttggc gatctcgagc ttctcctccg cggtgtagcc cgcgagctgg
     3121 atcacctcca tgcggtcgcg cagcggcggc gggatcggct cgagctggtt cgccgtggtg
     3181 atgaacatga cgttcgagag atcgaacggc aggtccagat agtggtcgcg gaaggtgccg
     3241 ttctgctcgg ggtctagcac ctcgagcatc gcgctcgacg ggtcgccgcg gaagtccgag
     3301 cccatcttgt cgatctcgtc gatcatgagg accgggttgt tggaccccgc gtcgcgcagc
     3361 gcgcgcatga tcgtgccggg gatcgcgccg atgtaggtgc ggcggtgacc gcggatctcg
     3421 gactcgtcgc gcaccccgcc gaccgagatg cgctcgaact cgcgccccat ggccgccgcg
     3481 atcgagcggc cgagcgaggt cttgcccacg cccggcgggc cgacgaagca gaggatcgcc
     3541 gagcgcgcgt cgggcttcag cttccgcacc gccaggaact cgatgatcct gtccttgatc
     3601 cggtcgatgt cgtagtggtc gcggtcgagg accgagcgcg cctccttgag gtcgaggtgg
     3661 tcctcggtcg acttcgacca cggcagggag acgatccact cgaggtaggt gcggatcacg
     3721 ccgtgctcgg ccgactgcgg cgggagccgc tcgaagcgct cgagctcgcg ctcggcctgc
     3781 ttcttcgcgt gctcgggcag ctccgccgcg tcgatctgct cgcgcagctc gcgcgcctcg
     3841 gcctgctgct cgtccacctc gccgagctcc tcctggatcg ccttgagctg ctggcggagg
     3901 aagtactcgc gctggccctt ctccatctcc gactgcacct gcgactggat ccgcgacccg
     3961 atcgagatca gctcggcctc gcgcgcgagc agctcggaga ggcgccgcag ccgcttggcg
     4021 aggctcgcct cggccagcag ctcctgccgc tcctcgggct tcacccgcag cgcgccggcg
     4081 atcatccacg acagctcgct cgggtcgtcc acgttcgcga cggccacctg aagctcctcg
     4141 ggcaggtacg ggacctgctg gatgatctgg ccgaacgtct gctgcacgtt gcgcacgagc
     4201 gcctcgagtt ccggcgtctc ctccaccacg tccggcatct cctcgatgcg cgcgacgagg
     4261 tagggcttct cggcggcgaa gccgttcagc ttcacgcgcg ggccgccctg cacgaggatc
     4321 cggagcgtgc cgtcgggcac cttgatcatg cgcgcgacgg tgcccgcgac gcccacgtcg
     4381 tagaggtcgt cggggcccgg ctcgtcgagg tcggggtcgc gcgcggtgac catcgcgagc
     4441 atccgctcgc ggccgagcac gtcgttcacg agctggatcg agcgctcctg gccgatcgcc
     4501 agcggcgtga gcgtgtgcgg gtaggcgacg gtgtcgcgca gcggcaggac cggtagcccg
     4561 tcgggcagcg cgccgtcgcc gttcgcctcg ccgctgcccg ggtgcgcgat gtggatgctc
     4621 acgcgggctc gcccggctcg cgcgagcgga tcggcacctg ccgcgggtcc tcggtcgcga
     4681 tcggcatctc cacgcgcagg atgccgtcgt cgtaggtggc gcttgcctcc tcggcgatca
     4741 cgtcggtgcc gagctcgacc acgcgccgga acgggccgtg cgcgatctcg atctgctggt
     4801 agagacggcc ctcggcgtcc tgcggcttgc gctcgcccgc gatcacgagc tggcggccgc
     4861 ggatctcgag gccgacgtcc tcgatgtcga tgccggcgag gtcagccttc accaccgcgc
     4921 gcggcgagcg gccgccgcag tagtacacgt ccacgggcgg cgagaagccg cgccggccgg
     4981 ggccgagccg ggcgcgctcg aacacgtcgc cgaagagctc gtcgatctca cggcgcatgc
     5041 gttcgaagtt ggcgaacaga tcgcgctcgg gcggcatcgc tcggagggta ttacccgcga
     5101 tcagagggtg tagacggcgc cctcgcgtgc caagtccacg cggtcgccga aggcgtcctc
     5161 ggcctcgttg cgcgcccaga gcgcgtcgag ctcgtcggag atgtgcgtga gcacgaggcg
     5221 gcgggcgccc gcggccttgc cgtgctcgcc ggcctcgcgc ggggtgaggt ggccgcgcgt
     5281 gccgctgcgc tcgggccgcg gcagggtggc ctcgacgagc agcaggtcgg agccgcgcgc
     5341 gaacgagacg aggtcgacgc tcggccggct gtcggcgccg aacgcgagct tgccgccgcc
     5401 gttggtcgac tcgacgctga tcgcgaacgt ctcggtgaaa tgcgggaccg actggaagct
     5461 cagctcgagc gagcccacgc gcggcgtgtc gtcctcgccg tactcctcga tcgtgaacgc
     5521 cttctcgatc aggtcctcgt tgccccacac gccgaccacg cgccggaagc agtcgagcgc
     5581 gccgcgcggc acgatcaggc gcggccgcgc cggcgcctcg gtgcccggcc agcggtccac
     5641 cggaacgggc tgctggcgcg gcgcgtaggt gagcgcgtac gagaacggga ccaggtcgag
     5701 ccagtggtcc gcgtgtatgt gcgagatgac gacggcatcg acccgcgtgt agtcgcggta
     5761 cttgcggagc ttgccgaaca cgccgttgcc gcagtcgacg acgacggtcg tgccgtcctc
     5821 ctcgacgaga tacccggagc acgcgcctcc ggcgtcctgc cacgacggtg acttcccgag
     5881 cacggtaagt cgcatctgcc gcgaactcta atccgcgcgg cgcgtgcgcg aagcggcatg
     5941 cagcacaccg cccgcggcgg gcgacggcgc cggactgccc gctactgcgg ttgcgggaac
     6001 gcgtacacgg cgcgcgtgcg ctcggtccgg aacacgaagg cgagctgacg ctcgggcttg
     6061 aggccgcgct cggcgcagcg ataggtcgcg catggcttgt ccacgttcaa ggcgcacagc
     6121 atgttgcgct tgaagtagca gtcgtcacag gac
//
LOCUS       DASVPL010000051         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_45609, whole genome shotgun sequence.
ACCESSION   DASVPL010000051 DASVPL010000000
VERSION     DASVPL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45609"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VF517_02825"
     CDS             complement(<1..831)
                     /locus_tag="VF517_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8051897.1"
                     /translation="MGFFSYVTGFGGRDMAVDLGTANTLVYVRGRGIVLSEPSVVAID
                     SRTGEVHAVGIEAKRMLGRTPGTIQAIRPLKDGVIADFDVTEQMLRHFIQKVHQNRWA
                     HPRVVVCVPSGVTGVEKRAVEEACLSAGARQAYLIEEPMAAAIGAGLPVSEPTGNMIV
                     DIGGGTTEVAVISLGGIVVSQSIRVGGDELDEAIINYAKREYKLLIGQQTAEEVKLEI
                     GSAFPLGEEVQAEIRGRDLVTGLPKTVVLSSAEIRQAIEVPLSQIIDAVKQTLDKTPP
                     E"
     gene            complement(966..1511)
                     /locus_tag="VF517_02830"
     CDS             complement(966..1511)
                     /locus_tag="VF517_02830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX8051898.1"
                     /translation="MLTLASRSPQRRAILEQLGVEFTVVPADVEEETSGDPRGLVVEN
                     ALRKARAVEGERVLGVDTDVVLDGHVFGKPADEDGAGMLLRRLAGRTHEVWSGIVLRS
                     GGAERTASACTRVRFRPLEEAELRWYLRSGEWRDRAGGYAIQGRGAALVEAIDGDFWN
                     VVGLPVPELLRLAPDLARERA"
     gene            complement(1511..>1865)
                     /gene="ndk"
                     /locus_tag="VF517_02835"
     CDS             complement(1511..>1865)
                     /gene="ndk"
                     /locus_tag="VF517_02835"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX8051899.1"
                     /translation="GEILARFERKGLTIAAMKLMTVDRELAERHYAEHSERPFFGELV
                     EFITSGPLVALVLEGHEAVTAARQLIGATNPLEATPNSIRGAFGLEVQTNLVHGSDSP
                     ESAKREIGLFFPELD"
BASE COUNT          258 a          690 c          644 g          273 t
ORIGIN      
        1 ctcgggcggg gtcttgtcga gcgtctgctt cacggcgtcg atgatctgcg acagcggcac
       61 ctcgatcgcc tggcggatct cggcgctcga gagcacgacc gtcttcggca gcccggtgac
      121 gaggtcgcgg ccgcggatct cggcctgcac ctcctcgccg agcgggaagg cggagccgat
      181 ctcgagcttg acctcctcgg ccgtctgctg gccgatgagg agcttgtact cgcgcttggc
      241 gtagttgatg atcgcctcgt cgagctcgtc tccgccgacg cggatcgact ggctcacgac
      301 gatgccgccc agcgagatga cggccacctc ggtggtgccg ccgccgatgt cgacgatcat
      361 gttcccggtg ggctccgaca ccgggaggcc ggcgccgatc gcggcggcca tcggctcctc
      421 gatgaggtac gcctgccggg cgccggccga caggcaggcc tcctcgaccg cgcgcttctc
      481 gacgccggtc acgcccgacg gcacgcagac caccacgcgc gggtgcgccc agcggttctg
      541 gtggaccttc tggatgaagt gccggagcat ctgctcggtc acgtcgaagt ccgcgatgac
      601 gccgtccttg agcggccgga tcgcctggat cgtgcccggc gtgcggccca gcatgcgctt
      661 ggcctcgatg cccaccgcgt ggacctctcc ggtgcgcgag tcgatcgcga cgaccgacgg
      721 ctcagacagc acgatcccgc gcccgcgcac gtagacgagc gtgtttgcgg tgcccagatc
      781 gaccgccatg tcgcggccgc cgaagccggt tacgtacgag aagaaaccca tcaggcgaag
      841 agttggctag ccactgctgg cgcgcccccc tggcgaaccg actcgggtgc gcgtcgtgcg
      901 ccggtccgcg agcggccaag tatacgagcc tgaatcgggc ctccaccgcg cctgcaactc
      961 acggatcagg cccgttcgcg cgccagatcg ggggccagcc ggaggagctc cggaacgggc
     1021 agcccgacga cgttccagaa gtcgccgtcg atcgcctcga cgagcgctgc gccgcgaccc
     1081 tggatcgcgt agccgcccgc gcggtcgcgc cactcgccgc tccgcaggta ccagcgcagc
     1141 tccgcctcct cgagcggccg gaagcgcacg cgcgtgcacg cgctggcggt gcgctccgcg
     1201 ccgccggacc gcaggacgat cccgctccac acctcgtggg tgcggcccgc caggcgccgc
     1261 agcagcatgc cggcgccgtc ctcgtcggcg ggcttgccga acacgtggcc gtcgagcacc
     1321 acgtcggtgt ccacgcccag cacgcgctcg ccctcgacgg cgcgggcctt gcgcagggcg
     1381 ttctcgacca ccagcccccg cgggtcgccg ctcgtctcct cctccacgtc cgccgggacg
     1441 acggtgaact cgacgcccag ctgctcgagg atcgcccggc gctgcggcga gcgcgaggcg
     1501 agggtgagca tcagtccagt tcggggaaga acagcccgat ctcgcgcttg gcggactccg
     1561 gggagtccga gccgtgcacg aggttggtct gcacctcgag cccgaaggct ccgcgaatcg
     1621 agttcggcgt cgcctcgagc gggttggtcg cgccgatgag ctgccgcgcg gcggtgaccg
     1681 cctcgtggcc ctcgaggacg agggcgacca gcggcccgct ggtgatgaac tccacgagct
     1741 cgccgaagaa cggccgctcg ctgtgctcgg cgtagtgccg ctccgccagc tcgcgatcca
     1801 ccgtcatcag cttcatcgcg gcgatcgtca ggcccttgcg ctcgaagcgc gcgaggatct
     1861 cgccg
//
LOCUS       DASVPL010000052         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_319074, whole genome shotgun sequence.
ACCESSION   DASVPL010000052 DASVPL010000000
VERSION     DASVPL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319074"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VF517_02840"
     CDS             <1..233
                     /locus_tag="VF517_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8051900.1"
                     /translation="ARHAEASSVSVDLRTGDEGTSLVVRDDGRGFDVDRRGPSSRGLG
                     RGGMAERARLVGGELTIDSRPGFGTELSLKVP"
     gene            233..868
                     /locus_tag="VF517_02845"
     CDS             233..868
                     /locus_tag="VF517_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8051901.1"
                     /translation="MLHILVADDHGIVRSGLKMLIERQDGMRVVAEADDGVAAVEAAM
                     RERPDVAVLDVSMPRMTGVQAAREIRARCPNTSVVLLSMHEEERYLFDGLKSGAQGYV
                     LKRAADEDLIEAVRSVAAGRPFLSAIAQTGAVKEWLDQGEKAPRDPLTPRELDVVKLI
                     AEAYTNKQIAEILKVSEKTVESHRANVLAKLGMRDRVELVRYAIRRGLIDA"
     gene            complement(835..1686)
                     /locus_tag="VF517_02850"
     CDS             complement(835..1686)
                     /locus_tag="VF517_02850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8051902.1"
                     /translation="MVVAVCTARPPDRVAEALAALRAQVPEGRLAVVANGRAPATGEL
                     HEPRPGLSHARNRALEWAAAEGADVVAYVDDDAVADPGWYEALARRWAEAPPAVACIG
                     GPIRPRFETQPPPWYSDAIRHVLTLLDRGPEVRDLDPEEEAVYGANISFRVEPLRAIG
                     GFDPGLGHSGRRVFFGEEDAVQRELHRRGYAIRYVPDAAVEHVIPPERLTRRSFVRRR
                     FAFGRALGMRRGRERGLAARKAVAGAGGAVIATMRGDGAVAMDRAVRAAENAGALYAS
                     IRPRRIA"
     gene            1756..2235
                     /locus_tag="VF517_02855"
     CDS             1756..2235
                     /locus_tag="VF517_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX8051903.1"
                     /translation="MVIYGVSFDMVGKQPIVLLKTRDGNKFLPIWIGHPEAAAILMKL
                     QGANTPRPMTHDLMDDMLTELDISCSRVAVTELKENTFYASITLQLNGREVEIDSRPS
                     DALALAVRTKAPIFAAEDVIADSAIEFEHEVEDQDEVVEKFKDFLDNVTPEDFAAGE"
     gene            complement(2350..>2825)
                     /locus_tag="VF517_02860"
     CDS             complement(2350..>2825)
                     /locus_tag="VF517_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051904.1"
                     /translation="DQGMWRDYVDSLNEIHRARRDAMLDALAEFFPAEATWTRPSGGL
                     FIWATLPDFIDTTDLLARALRENVAFVPGEGAFLDGRGRSSMRLNFSGSDEDTIREGI
                     RRIGEVVAEQVALYGTLTGEESPTAKRPAPKPAQKEAAKPPTDGAQVVPMRRRAG"
BASE COUNT          401 a         1019 c         1002 g          403 t
ORIGIN      
        1 tggcgcgaca cgccgaggcg tcgtcggtat ccgtcgacct gcgcacggga gacgagggca
       61 cttctctggt ggtgcgcgac gacggccgcg gcttcgacgt cgaccgccgc ggccccagct
      121 cgcgtggcct cggccgcggc ggcatggccg agcgcgcgcg gctcgtgggc ggcgagctga
      181 cgatcgactc gcgaccgggc ttcggcaccg agctgtcgct gaaggtgccg tgatgctgca
      241 catcctcgtg gccgacgacc acggcatcgt ccgctccggg ctgaagatgc tgatcgagcg
      301 ccaggacggc atgcgcgtgg tggcggaggc cgacgacggc gtcgccgccg tcgaggccgc
      361 catgcgcgag cggcccgacg tggccgtgct cgacgtctcg atgccgcgca tgaccggcgt
      421 gcaggccgca cgcgagatca gggcccgctg cccgaacacc tcggtcgtcc tgctctcgat
      481 gcacgaggag gagcgctacc tcttcgacgg gctgaagtcg ggcgcccagg gctacgtgct
      541 caagcgcgcc gccgacgagg acctgatcga ggccgtccgg tcggtcgccg cgggccgtcc
      601 gttcctctcg gcgatcgccc agaccggagc ggtgaaggag tggctcgacc agggcgagaa
      661 ggccccgcgc gacccgctca cgccgcgcga gctcgacgtg gtcaagctga tcgccgaggc
      721 ctacacgaac aagcagatcg ccgagatcct gaaggtctcg gagaaaaccg tcgagtcgca
      781 ccgcgcgaac gtgctggcga agctcggcat gcgcgaccgc gtggagctcg tgcgctacgc
      841 gatccgccgc ggcctgatcg acgcgtagag cgccccggcg ttctcagccg cgcgcacggc
      901 gcggtccatc gccacggcgc cgtctccccg catggtcgcg atcaccgctc ctcccgcccc
      961 ggcgaccgcc ttacgcgcgg caaggccgcg ctctcggccg cgcctcattc ccagcgcccg
     1021 tccgaaggca aagcggcgcc tgacgaagga gcgccgggtg aggcgctcgg gggggatcac
     1081 atgctcgacc gcggcgtccg ggacgtagcg gatcgcgtac ccgcggcggt gcagctcgcg
     1141 ttggacggcg tcctcctcgc cgaagaagac gcgccggccg ctgtgtccga gcccggggtc
     1201 gaacccgccg atcgcgcgca gcggctcgac ccggaacgag atgttcgccc cgtagacggc
     1261 ctcctcctcg gggtcgaggt cgcgcacctc ggggccgcgg tcgagcagcg tgaggacgtg
     1321 gcggatcgcg tccgagtacc acggcggcgg ctgggtctcg aagcgcgggc ggatcgggcc
     1381 gccgatgcag gcgaccgcgg gcggcgcctc ggcccagcgc cgggcgagcg cttcgtacca
     1441 gcctgggtcg gcgacggcgt cgtcgtccac gtacgcaacc acgtcggcac cctccgctgc
     1501 ggcccactcg agcgcacggt tgcgggcgtg tgagagcccc gggcggggct cgtggagctc
     1561 gcccgtggcg ggggcgcgcc cattcgcgac gacggccagc cggccctccg gaacctgggc
     1621 gcgcagggcc gccagcgcct cggccacccg atcgggcgga cgggcggtgc agacggcgac
     1681 taccacgccg cctcctgatc tagactctgg ctcgccctct ccacccgctt tccgtttggg
     1741 gtccctttgc aggaaatggt catatacggg gtgagcttcg acatggtcgg caagcagccc
     1801 atcgtcctgc tcaagacccg ggacggcaac aagttcctcc cgatctggat cggccatccc
     1861 gaggccgcgg cgatcctgat gaagctccag ggagcgaaca ccccgcgccc gatgacccac
     1921 gacctcatgg acgacatgct caccgagctc gacatctcgt gctcacgggt cgcagtcacg
     1981 gagctgaagg agaacacgtt ctacgcgtcg atcacgctcc agctcaacgg gcgcgaggtc
     2041 gagatcgact ctcggccctc cgacgcgctg gcgctcgccg tgcgcaccaa ggcgccgatc
     2101 ttcgcggccg aggacgtgat cgccgactcg gccatcgagt tcgagcacga ggtcgaggac
     2161 caggacgagg tcgtcgagaa gttcaaggac ttcctcgaca acgtcactcc tgaagacttt
     2221 gcggctggcg agtagcgggc cgctgcaaac cggcgcctgg cggcgtcatc ggccactcgc
     2281 gtggctctgc cacgcgtcgc ggcctgcttc cttgccaggc ttggttttcg cggcctgcgg
     2341 ttctccggct taaccagctc ggcgccgcat cggcaccacc tgcgcgccgt cggtcggcgg
     2401 cttcgccgcc tccttctgcg caggcttcgg agccgggcgc ttcgcggtcg gactctcctc
     2461 gcccgtgagc gtgccgtaga gcgcgacctg ctcggcgacc acctcgccta tccggcggat
     2521 cccctcgcgg atcgtgtcct cgtcggaccc cgagaagttc agccgcatcg agctgcgtcc
     2581 gcgcccgtcg aggaacgcgc cctcgccggg cacgaacgcg acgttctcgc gcagggcgcg
     2641 cgcgaggagg tcggtggtgt cgatgaagtc cggcagcgtg gcccagatga agaggccgcc
     2701 gctcggccgg gtccacgtgg cctcggcggg gaagaactcg gccagcgcgt cgagcatcgc
     2761 gtcgcggcgg gcgcggtgga tctcgttcag cgagtcgacg tagtcgcgcc acatgccctg
     2821 gtcga
//
LOCUS       DASVPL010000053         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_6564, whole genome shotgun sequence.
ACCESSION   DASVPL010000053 DASVPL010000000
VERSION     DASVPL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6564"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1737
                     /locus_tag="VF517_02865"
     CDS             190..1737
                     /locus_tag="VF517_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051905.1"
                     /translation="MLPWTRSKWFRRGVTGFSTVFVAVVLATSLMAEGSALKRADVEY
                     DAVDCVKDKNTEGRARPATGCKRKKRVVIPIKDGAAVSKEDLERALDEMPRLQGPRGL
                     QGARGEHGAAGRRGPAGTRGANGVGRAGRPGTDGDDGAAGRQGAQGARGSQGSDGERG
                     ARGETGETGDTGATGATGATGATGATGATGDKGELGETGPQGPKGETGSAGPQGASGP
                     AGPQGIQGETGAQGGLGPQGPQGDVGPQGVQGETGTQGPQGIQGETGAQGAQGIQGET
                     GAQGPQGVQGIQGETGAQGAQGIQGETGPTGPQGPKGDTGPAGPQGIQGATGAQGPQG
                     VAGPQGETGPTGPTGPQGNTGPAGPAGPQGTTGATGATGSQGPQGAIGPQGPTGATGA
                     TGATGATGAQGPTGPQGADAPRRWAVVASDGTLVRSSGAGVSSTRVGFSQYRVDFGTD
                     VSNCMYLATVADPGGSMGDHGVARPRRNSANTNQVMVETVGDLDGFGSDPAIGPRDLS
                     FHIAAFC"
     gene            1742..>2855
                     /locus_tag="VF517_02870"
     CDS             1742..>2855
                     /locus_tag="VF517_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8051906.1"
                     /translation="MSSPAPKRSHLDSVPRPVSAFVAAIRGVAAGVTLIYAACLLTGI
                     GHEWSDQGLTHAINSGLLATAAFLCLSRSALARAQRIAWFVGGLALASWSAGEVLFAL
                     GHPGGPFSTANLLSLGFYPLACLALSLMLRAQLESFFTTLWLDGLAGALSASALVAAF
                     VFPPVLANSQGSTTEILTSISYPLADLLLVAFVLFALAMTGWRPSATLGGACAAFVVI
                     AITDAFSLWAAATSNSTRNELDWLWPAAALGLAECAWRAAPAVKRISSTSLRLLMFPV
                     VVSLTALGLLLSGLVNELHMAGYELATASLFLIVVRLALTVLENLQIADASKREALTD
                     ALTGLGNRRRLMQDVEAALEHGGTTLLVLYDLDGFKG"
BASE COUNT          391 a         1087 c         1018 g          359 t
ORIGIN      
        1 cgagcgtcgg gatgatccac tcctacgggc ggccgggctt cagcgacacg atcgtggtcg
       61 agacgttcga gggccagcac ccaagcggcg gcaaccccgg cgaccgtccg ttccacgtcg
      121 ccgtctactg ctgagagaaa cctgcccgat gccgtcaagg acgcggctcc ggccaccgat
      181 agaacctgaa tgcttccttg gacgcgctcc aagtggttcc gccgtggagt caccggattc
      241 tcgaccgtgt tcgtcgcggt ggtcctcgcg acgtccctca tggccgaggg cagcgcactc
      301 aagcgcgcgg acgttgagta cgacgccgtc gactgcgtca aggacaagaa caccgagggc
      361 cgcgccaggc ctgccacagg ctgcaagcgg aagaagcgcg tcgtgattcc gatcaaggac
      421 ggcgccgccg tctcgaagga ggacctcgag cgggcgctcg acgagatgcc gcgccttcag
      481 ggcccgcgtg gcctccaggg cgcccgcggc gagcacggcg cggccggacg gcgcggaccc
      541 gccggcacac gcggcgccaa cggcgtcggc cgggccgggc ggccgggcac cgatggtgac
      601 gacggcgcgg ccgggcgcca aggtgcgcag ggcgcgcgcg gcagccaggg ctccgacggc
      661 gagcgcggcg cgcgcggcga gaccggtgag accggcgaca cgggtgcaac gggtgccacg
      721 ggtgcaacgg gtgccacggg tgcgacgggt gcgacgggcg acaagggaga acttggcgag
      781 accgggccgc agggcccgaa gggtgagacc ggatcggccg gccctcaggg cgcgagcggc
      841 ccggccggcc cgcagggcat ccagggcgag actggagctc agggcggcct cgggccacag
      901 ggcccgcagg gcgacgtcgg cccgcaggga gttcagggcg agaccggcac gcagggtcct
      961 caggggatcc agggcgagac cggagcgcag ggcgcccagg gcatccaggg cgagaccggc
     1021 gcgcagggcc cccagggagt tcagggcatc cagggcgaga ccggagcgca gggcgcccag
     1081 ggcatccagg gcgagaccgg cccgaccggt ccgcagggcc cgaagggcga caccgggccc
     1141 gcgggcccgc agggaattca gggcgcgacc ggggcgcagg gaccgcaggg cgtcgccggg
     1201 ccccagggcg agaccgggcc gaccggcccc accggccccc agggcaacac cggccccgcc
     1261 ggcccggccg gcccgcaggg cacgaccggc gcgacgggcg cgaccgggtc ccagggcccg
     1321 cagggcgcga tcggcccgca gggaccgacc ggcgcaactg gcgcgaccgg cgcgaccggc
     1381 gcgaccggtg cccagggccc caccggcccg cagggtgccg acgccccgcg gcgctgggcc
     1441 gtggtcgcct ccgacggcac cctcgtgcgc tcgagcggcg cgggagtcag ctccacccgt
     1501 gtcggcttct ctcagtaccg cgtcgacttc ggcaccgacg tctcgaactg catgtacctc
     1561 gccacggtcg ccgatccggg cggctcgatg ggcgaccacg gcgtcgcgcg cccgcggcgc
     1621 aacagcgcga acacgaacca ggtgatggtc gagacggtcg gtgacctcga cggcttcggc
     1681 tccgaccccg ccatcggccc gcgcgacctc tccttccaca tcgcggcctt ctgctgatcc
     1741 gatgagctcc cccgccccca agcgctctca cctcgactcg gtcccgcgtc cggtctcggc
     1801 gttcgtcgcc gcgatccgcg gcgtcgcggc cggcgtcacg ctcatctacg ccgcgtgcct
     1861 gctcacgggc atcggccacg aatggagcga tcagggactg acccacgcga tcaacagcgg
     1921 actgctcgcc accgccgcct tcctctgcct gtcgcgctcg gcgctcgcgc gcgcccagcg
     1981 gatcgcctgg ttcgtgggcg gcctcgcgct ggcaagctgg tctgccggcg aggtgctgtt
     2041 cgcactgggc cacccgggcg gaccgttctc gacggccaac ctgctctcgc tcggcttcta
     2101 cccgctcgcc tgcctggcgc tctcgctgat gctgcgcgct cagctcgagt cgttcttcac
     2161 cacgctgtgg ctcgacggcc tcgccggcgc gctgtccgcg tcggcgctgg tcgccgcgtt
     2221 cgtcttcccg ccggtcctcg cgaactcgca ggggagcacg accgagatcc tcacctcgat
     2281 cagctacccg ctggccgacc tgctgctcgt cgctttcgtc ctcttcgcgc tcgccatgac
     2341 cggctggcgt cccagcgcga ccctgggcgg tgcctgcgcc gcgttcgtcg tgatcgcgat
     2401 caccgatgcc ttctccctct gggctgccgc caccagcaac tccacgcgca acgagctcga
     2461 ctggctctgg ccggccgccg cgctcggcct ggccgagtgc gcctggcgcg ccgcgcccgc
     2521 ggtcaagcgg atcagcagca cgagtctgcg cctgctgatg ttccccgtgg tcgtctcgct
     2581 gaccgcgctc gggctgctgc tctccggcct cgtcaacgag ctgcacatgg ccggctacga
     2641 gctggcgacc gcctcgctct tcctgatcgt ggtccggctg gcgctcaccg tgctcgagaa
     2701 cctccagatc gccgacgcca gcaagcgcga ggcactcacc gacgccctca ccggcctagg
     2761 caaccgccgc cgcctgatgc aggacgtcga ggccgcgctc gagcacgggg gcaccacgct
     2821 gctcgtcctc tacgacctcg acggcttcaa gggct
//
LOCUS       DASVPL010000054         6791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_359393, whole genome shotgun sequence.
ACCESSION   DASVPL010000054 DASVPL010000000
VERSION     DASVPL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359393"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..950)
                     /gene="murB"
                     /locus_tag="VF517_02875"
     CDS             complement(42..950)
                     /gene="murB"
                     /locus_tag="VF517_02875"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX8051907.1"
                     /translation="MSAPPGAEAHYPLARLTTIRTGGRADWFARPDRSERLADLLGWA
                     HEQRVEVGVVGSGSNLLVADEGFRGLVLKLDGELATIEPEGRRLLCGGGARLPKASAR
                     AASLGLTGLEFGVNIPGTVGGAVRMNANAYGGDLSRVLEWVEVASAEGVGRRGPDELG
                     FSYRRSNLRPGEVVARASFALDPAEPAAVKRTLAEMRAQRKAAQPSGIKTFGSTFKNP
                     DDARAEGRSAGQLLDAAGCRGLAVGGARFSEKHANFVENMGSATTADVVALMVEGRRR
                     VRERFGVELEPEVQLLGDVDATGLYS"
     gene            complement(947..2302)
                     /gene="murC"
                     /locus_tag="VF517_02880"
     CDS             complement(947..2302)
                     /gene="murC"
                     /locus_tag="VF517_02880"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX8051908.1"
                     /translation="MAPWTGRRLHFVGIGGAGMSGLALVAHALGASVTGSDRAWSPYC
                     EPLVAAGVSPVVGHDAANVPDGAEVVVSTAIAPDNPELAAAGVVLHRADLLAELTRLK
                     PCIAVGGTHGKTTTASMAAHVLLETGRSPAYLIGGELRTTGLNAAWGEGEWIVVEADE
                     SDRSFLKLAPRVAVVTNTELDHHATYGSQADIEAAYAEFAGLAEVVIAPPDAGLGGIG
                     FGIGEGEVAARDVKLGPDGSRFVVDGVSLALRVPGLHNVQNALAALAACREAGVGLEE
                     AAPALATFPGAGRRFEWRGRTASGAVVYDDYAHHPTEVRATLEAARTLGAARVVACFQ
                     PHLFSRTEALAREFGAALGLADVACVLDVYPARELAADFPGVSGWLVARAAADAAGGR
                     PVFWTPSLEDAERALTAELREGDLLLTLGAGDVDRLAARLVAEAEPPSVTAAGEGTAP
                     S"
     gene            2572..3237
                     /locus_tag="VF517_02885"
     CDS             2572..3237
                     /locus_tag="VF517_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX8051909.1"
                     /translation="MPWDEITPRQFDSMEKDLDGISKKTMQDHYKLYEGYCKKTNECR
                     KILNEFDYSEIEGNQVYSALRAVSVDYTFALLGMKNHELYFGHLGGSGGEPNGRFAEL
                     VEQELNGVDKWKDAVRKATKAARGWVMVGYDLNDGSLFNYIMDTQNLWAVYNMVPVLA
                     IDVYEHAYAADFGATPDGRGEYVEAFFRNLDWDHVNRQVAAAEAAKQGADSSSATGGG
                     EDA"
     gene            3295..3627
                     /locus_tag="VF517_02890"
     CDS             3295..3627
                     /locus_tag="VF517_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8051910.1"
                     /translation="MTTLDVTTTDIPRGVHVKLAGELDLATVPKLDDEMKRIEDAGPE
                     LILLDLQALSFMDSSGLRSLLGADGRAQEAGRRLVLVRGDERVQRVLRITRLDERLEI
                     VDSPEAVG"
     gene            complement(3640..4104)
                     /locus_tag="VF517_02895"
     CDS             complement(3640..4104)
                     /locus_tag="VF517_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8051911.1"
                     /translation="MRNPAKFESRVTGSRLHAVALAEGAAGTGRTGMSVELEPGTMAA
                     GEARAALAVLDGRIDRGILDDIRLLVSELVTNSVRHARGAGRELVGLDVVRSGGTVRV
                     EVSDGGTGFQPRARTKAHDEPGGWGLHLVDRLADRWGVEAGRRARVWFEIDR"
     gene            complement(4185..5291)
                     /gene="murG"
                     /locus_tag="VF517_02900"
     CDS             complement(4185..5291)
                     /gene="murG"
                     /locus_tag="VF517_02900"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX8051912.1"
                     /translation="MSAPRVAIAAGGTAGHVVPALAIADALRDSGAQVEFVGGERAEA
                     ELVPQAGYPFHRLAVAGLDRRNPLKAARAVGLAGAAVLRARALLREIGADAVIGGGGY
                     VAGPVGLAAVSLRIPLVLTEADSHLGVSNRLLARFARRVFLAFPIEGRAGERYEVVGR
                     PVPRDTGRVERGAARARLGLSEDAPCLLVFGGSIGARTLNDAALEAFAAPGSPCQVLH
                     ACGRRDHPDLRRRLDEAGSPPHYRLFAYVTPFADALAAADLAVARSGGSVFELAAAGV
                     PSILVPYPHATADHQAGNARYMAAAGAAVVMGDDEVSAERLRTEVTALLAAPERLEAM
                     RAAARAAGRPDAADRVAAAALESAGRVFATHARE"
     gene            complement(5203..6465)
                     /gene="ftsW"
                     /locus_tag="VF517_02905"
     CDS             complement(5203..6465)
                     /gene="ftsW"
                     /locus_tag="VF517_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX8051913.1"
                     /translation="MAASTRARKRAKPPLEYSILYTATLCLLAAGAVMVYSASSAESL
                     QSGGDAAGYLKRYVVFGALGLVAMHVLSRRSLTVARNATPLILGAGFVLTLAVMVPGV
                     GVTVNGATRWLGAGPVQFQPSELLKIGVIMFGAAVLAARPSRLESVGAMFKPLLLVVG
                     AAALILLKQPDMGTAMVICFSMFALLLAAGARIRHLATIGGTLMVAAMLLAIAEPYRR
                     DRLLSFIDPWGDAGDTGFQGVQAMIAIGSGGMFGVGLGESVQKIHYLPEAHTDMILAI
                     VGEELGLFGIVSLAALYGLIAYAGFRAAKLAKDRYAKLLAAGITGLILTQAVINFFAV
                     MGMAPLTGVPLPFISYGSSNMVVLLGAMGLLLNVASNPGQAAARRSDGKLRVVDGGRG
                     ERSTRRDSGGRDGGARRSRTGDRRRAAG"
     gene            complement(6499..>6791)
                     /locus_tag="VF517_02910"
     CDS             complement(6499..>6791)
                     /locus_tag="VF517_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051914.1"
                     /translation="ILGGSLKGESFAGLREPLAERAVAAYLIGAAADELQSALEGAVA
                     LHRAGDLETAVAAARAAASPGDVVLLSPACASYDQYSDYEARGDHFRRLATG"
BASE COUNT          954 a         2565 c         2478 g          794 t
ORIGIN      
        1 cgcgcaccgg cgcggcgagc cgtgcgcgtg gggctccgcc ctcacgagta cagccccgtc
       61 gcgtcgacgt cgcccaggag ctgcacctcg ggctcgagct cgaccccgaa gcgctcgcgc
      121 acgcggcggc gcccctccac catcagcgcc accacgtccg ccgtggtggc cgagcccatg
      181 ttctcgacga agttggcgtg cttctcggag aagcgggcgc cgccgaccgc cagcccgcgg
      241 cagccggccg cgtcgaggag ctgacccgcg ctgcgtccct ccgcgcgcgc gtcgtccggg
      301 ttcttgaagg tcgagccgaa ggtcttgatg cccgagggct gcgccgcctt gcgctgggcg
      361 cgcatctcgg caagcgtgcg cttcaccgcg gctggctcgg cgggatcgag cgcgaacgag
      421 gcgcgcgcca ccacctcgcc ggggcgcagg ttcgagcgcc ggtacgagaa gccgagctcg
      481 tccggcccgc ggcggccgac gccctcggcg ctcgccacct cgacccactc gagcacgcgc
      541 gagaggtcgc cgccgtaggc gttggcgttc atccgcaccg ccccgccgac ggtccccggg
      601 atgttcacgc cgaactcgag cccggtcagc ccgaggctcg ccgcgcgggc cgacgccttc
      661 gggagccggg cgccgccgcc gcagagcagc cgccgaccct ccggctcgat cgtggccagt
      721 tcgccgtcca gcttcagcac gagtcctcgg aagccctcat ccgccaccaa caggttcgag
      781 ccggacccca ccactcctac ctcgacccgc tgctcgtgcg cccatccgag taggtcggcg
      841 agccgctccg agcggtccgg ccgggcgaac cagtcggcgc ggccgcccgt gcggatcgtg
      901 gtgagccgcg cgagcgggta gtgggcctcg gctcccggcg gagcgctcac gacggggccg
      961 tcccctcccc agccgccgtg accgaaggcg gctccgcctc cgcgacgagc ctggcggcga
     1021 gccgatcgac gtccccggcg ccgagcgtga gcagcagatc cccctcccgc agctccgccg
     1081 tgagcgcccg ctccgcgtcc tcgagcgacg gcgtccagaa caccgggcgt ccgcccgccg
     1141 cgtccgccgc cgcccgcgcc acgagccagc cggacacgcc cgggaagtcc gccgccagct
     1201 cgcgcgccgg gtacacgtcg agcacgcagg cgacgtcggc cagccccagc gcggcgccga
     1261 actcgcgcgc gagcgcctct gtgcgcgaga agaggtgcgg ctggaagcag gccaccacgc
     1321 gcgcggcgcc cagcgtgcgc gccgcctcga gcgtggcgcg cacctcggtc gggtggtggg
     1381 cgtagtcgtc gtagaccacc gcgcccgacg ccgtgcgccc gcgccactcg aaccgccgcc
     1441 ccgcgcccgg gaaggtggcg agcgccggcg cggcctcctc gaggcccacc ccggcctcgc
     1501 ggcacgccgc cagcgccgcc agcgcgttct gaacgttgtg cagccctggc accctcagcg
     1561 cgaggctcac gccgtcgacc acgaaccgcg acccgtccgg cccgagcttg acgtcgcgcg
     1621 cggccacctc gccctcaccg attccgaagc cgatcccgcc gaggccggcg tccggaggcg
     1681 cgatcacgac ctccgccagc cccgcgaact ccgcgtacgc cgcctcgatg tcggcctgcg
     1741 acccgtaggt cgcgtggtgg tcgagctcgg tgttggtcac caccgccacc cgcggcgcga
     1801 gcttcaggaa cgagcggtcg gactcgtcgg cctcgaccac gatccactcc ccctcccccc
     1861 acgccgcgtt cagcccggtc gtgcgcagct ccccgccgat caggtacgcg ggcgagcggc
     1921 cggtctcgag gagcacgtgc gcggccatcg aggcggtggt ggtcttgccg tgcgtgccgc
     1981 ccacggcgat gcagggcttg agccgggtca gctccgcgag caggtcggcg cggtggagca
     2041 cgacgccggc cgccgccagc tccgggttgt cgggtgcgat ggcggtcgag accacgacct
     2101 cggccccatc gggcacgttc gccgcgtcgt gcccgaccac gggcgatacg cccgcggcga
     2161 ccagcggctc gcagtacggc gaccaggccc ggtcggaccc cgtcaccgaa gcgccgagcg
     2221 cgtgcgcgac cagcgccagc ccgctcatcc cagcaccgcc gatgccgacg aagtgcagtc
     2281 gccggccggt ccagggagcc accccggcga cattagcggc gcgggcggag agctagatct
     2341 gcgggccggg gcccggcggc atcggcgcgg ggcccggcgc gggaggagcg ggctcgggcc
     2401 ccggcggcag cggcggtccg gggccggggc cgggtgtcgg cggcgacggg tcggggccgg
     2461 gcggggccgg ggccggaggg cgttcggcgc gcgtgctcac cgggccatcg tcgcacacgc
     2521 tacgtcggta gggtccgacg tcgaatcgca ttacgaggaa aggacgggca aatgccctgg
     2581 gacgagatca cgccccgcca gttcgactcc atggagaagg acctggacgg gatctccaag
     2641 aagacgatgc aggaccacta caagctctac gaggggtact gcaagaagac caacgagtgc
     2701 cgcaagatcc tgaacgagtt cgactactcg gagatcgagg gcaaccaggt ctactcggcg
     2761 ctgcgcgcgg tgtccgtgga ctacaccttc gcgctgctcg ggatgaagaa ccatgagctc
     2821 tacttcggcc acctgggcgg gagcgggggc gagccgaacg gccgcttcgc cgagctcgtc
     2881 gagcaggagc tgaacggcgt cgacaagtgg aaggacgccg tccgcaaggc gaccaaggcc
     2941 gcccgcggct gggtcatggt cggctacgac ctgaacgacg gctcgctctt caactacatc
     3001 atggacaccc agaacctgtg ggccgtctac aacatggtcc cggtcctggc gatcgacgtg
     3061 tacgagcacg cctacgcggc ggacttcggc gcgacgccgg acgggcgcgg ggagtacgtc
     3121 gaggcgttct tccgcaacct cgattgggat cacgtgaacc gtcaggtcgc cgccgcggag
     3181 gccgccaagc agggcgccga ctcgagctcc gcgacgggtg gaggcgagga cgcgtaatag
     3241 ggacgatcgt ggggggcgcg ccagggtaat gtctgcccgg ggcgcgcccc gccgatgacc
     3301 accctggacg tcaccacgac cgacatccct cgcggtgtcc acgtgaagct cgcgggcgag
     3361 ctcgacctcg cgaccgtgcc gaagctcgac gacgagatga agcggatcga ggacgccggg
     3421 cccgagctga tcctgctcga cctccaggcg ctgtcgttca tggactcgag cggcctgcgg
     3481 tcgctcctgg gcgccgacgg ccgcgcacag gaggccggac ggcgcctcgt gctggtgcgc
     3541 ggcgacgagc gcgtccagcg cgtgctgcgg atcacccgcc tcgacgagcg gctcgagatc
     3601 gtggacagcc ccgaggccgt cggctagccg gtcgcgccgc tagcggtcga tctcgaacca
     3661 gacgcgcgca cggcgcccgg cctcgacgcc ccagcggtcg gccaggcggt cgaccaggtg
     3721 gagcccccag ccgccgggct cgtcgtgcgc cttggtgcgc gcgcgcggct ggaagccggt
     3781 cccgccgtcg gagacctcga cgcggacggt gccgccgctg cgcaccacgt cgagcccgac
     3841 cagctcgcgc cctgcgccgc gggcgtggcg gacgctgttg gtgacgagct cgctcacgag
     3901 caggcggatg tcgtcgagga tgccgcggtc gatgcggcca tcgagcacgg cgagcgcggc
     3961 ccgcgcctcc ccggccgcca tcgtgccggg ctcgagttcc acgctcatcc cggtccggcc
     4021 cgtccccgcg gcgccctcgg caagcgcaac ggcgtgcagg cggctgccag tcacccggct
     4081 ctcgaacttc gctgggtttc gcatttcgct cgcacgcaca gtcgcttggc gggccatctg
     4141 gggaagctca cctcaggggg gtcagggcgc tccgtccggg tgggttattc ccgggcatgg
     4201 gttgcgaaaa cacgtccggc gctctccagg gcagccgccg ccacccggtc ggccgcgtcg
     4261 ggccgtccgg cggcgcgggc ggcggcgcgc atcgcctcga gccgctctgg cgccgccagc
     4321 agcgccgtga cctcggtgcg cagccgctcg gcgctcacct cgtcgtcacc catcaccacg
     4381 gcggcgcccg cggcggccat gtaacgggcg ttgccggcct gatggtcggc ggtcgcgtgc
     4441 gggtagggga cgaggatcga gggcacgccg gcggcggcca gctcgaagac ggagccgccc
     4501 gagcgcgcga ccgcgaggtc ggccgcggcg agcgcgtccg cgaacggggt cacgtacgcg
     4561 aacaggcgat agtgcggcgg cgagccggcc tcgtcgagcc ggcggcgcag gtccgggtgg
     4621 tcgcggcgcc cgcaggcgtg caggacctgg cagggggagc cgggggcggc gaacgcctcg
     4681 agcgcggcgt cgttgagggt gcgagcgccg atcgagccgc cgaagacgag caggcacggg
     4741 gcgtcctcgc tcagccccag gcgcgcacgc gcggcgccgc gctcgacgcg cccggtgtcg
     4801 cgcggcacgg gccggccgac cacctcgtag cgctcgcccg cgcgcccctc gatcgggaac
     4861 gcgaggaaga cgcgccgcgc gaagcgcgcg agcaggcggt tcgagacgcc gaggtggctg
     4921 tcggcctcgg tcagcaccag cgggatccgc agcgagaccg ccgccagccc gacgggcccg
     4981 gccacgtagc cgccgccgcc gatcacggcg tccgccccga tctcgcgcag aagcgcccgc
     5041 gcgcggagca ccgccgcccc ggcgagcccg acggcacgtg cggccttcag cgggttgcgc
     5101 cggtcgagcc cggccaccgc cagccggtgg aacgggtagc cggcctgcgg cacgagctcc
     5161 gcctcggcgc gctcgccgcc gacgaactcg acctgcgcgc cgctatcccg cagcgcgtcg
     5221 gcgatcgcca gtgcgggaac gacgtgcccc gccgtcccgc ccgccgctat cgcgacgcgt
     5281 ggagcgctca ccgcgtccac cgtccaccac gcgcagcttg ccgtcgctgc gccgcgcggc
     5341 cgcctgcccc gggttcgagg cgacgttcag cagcaggccc atggcgccga gcagcacgac
     5401 catgttcgag ctgccgtagg agatgaacgg cagcggcaca ccggtgagcg gcgccatgcc
     5461 catcacggcg aagaagttga ttaccgcctg cgtgaggatc aggccggtga tcccggcggc
     5521 cagcagcttc gcgtagcggt ccttggcgag cttggccgcg cggaagccgg cgtacgcgat
     5581 cagcccgtac agcgccgcca gcgagacgat cccgaacagg ccgagctcct cgcccacgat
     5641 cgccaggatc atgtccgtgt gggcctcggg caggtagtgg atcttctgca ccgactcgcc
     5701 gaggccgacc ccgaacatgc ccccggagcc gatcgcgatc atcgcctgga cgccctggaa
     5761 gccggtgtcg cccgcgtccc cccacgggtc gatgaacgag agcaggcgat cgcgccggta
     5821 gggctcggcg atcgccagga gcatcgccgc gaccatcagc gtgccgccga tcgtggccag
     5881 gtggcggatg cgcgcgccgg cagccaggag cagggcgaac atcgagaagc agatgaccat
     5941 cgcggtgccc atgtcgggct gcttgagcag gatcagcgcg gccgcgccca ccacgagcag
     6001 cagcggcttg aacatcgcgc cgaccgactc gagccgcgac ggtcgcgcgg ccagcacggc
     6061 ggccccgaac atgatcacgc cgatcttcag gagctcggag ggctggaact ggaccgggcc
     6121 ggcgccgagc cagcgcgtgg cgccgttcac ggtcacaccc acgcccggca ccatcacggc
     6181 cagcgtgagc acgaacccgg ccccgaggat gagcggcgtg gcgttgcggg cgacggtcag
     6241 gctgcggcgc gagagcacgt gcatggcgac caggccgagc gcgccgaaca ccacgtagcg
     6301 cttgaggtag ccggcggcgt cgccgcccga ctggagcgac tcggccgacg aggccgagta
     6361 gaccatcacc gcgcccgccg ccagcaggca cagcgtggcc gtgtagagga tcgagtattc
     6421 gaggggcggc ttcgcgcgct tgcgcgcgcg cgtggaggcg gccatccggg acccgttcga
     6481 cgcgcggcgg cggaatcctc atccggtggc gagcctcctg aagtggtccc cacgggcctc
     6541 gtagtcgctg tactggtcgt agctcgcaca ggccggtgag agaagcacca cgtcgcccgg
     6601 actcgcggcc gcgcgcgcgg ccgccacggc ggtctcgaga tcgcccgcgc ggtgcagggc
     6661 gaccgcgccc tcgagggcgc tctgcagctc gtccgccgcg gccccgatca ggtacgccgc
     6721 cacggcgcgc tcggcgagcg gctcgcgcag cccggcgaag ctctcgccct tgaggctgcc
     6781 gcccaggatg g
//
LOCUS       DASVPL010000055         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_202038, whole genome shotgun sequence.
ACCESSION   DASVPL010000055 DASVPL010000000
VERSION     DASVPL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202038"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3286)
                     /locus_tag="VF517_02915"
     CDS             complement(<1..>3286)
                     /locus_tag="VF517_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8051915.1"
                     /translation="IDAPTSDGSSASLAGSVHDLTARTLALLQSWLAAEPLAESRLVL
                     LTDNALATSGVESPNLAQAALLGLVRSAHSENPGRFGVIDLDGADASASALAGALAAE
                     EPELALREGRALAPRLARASGLIPPTAGAWRLGIERTGSLEDLTLVPSDAADAPLAEG
                     HVRVAMRAGGLNFRDVLIALGMYPGEAPLGSEGAGVVVETAADVEHLQPGDRVMGIVR
                     EPFGPYGVTDHHMLVPIPDAWSFAEAASVPSVFATAYYALVDLAGLKQGERILIHAAA
                     GGVGMAAVQIAKHLGAEVYATASPAKWDTVRGLGVPDDHIASSRDLDFRDRFLDATEG
                     AGMDVVLDALAREFVDASLDLLPQGGRFVEIGKADIREPDEVAAQHPGVRYRAFDLAE
                     AGPDRIQAMLLEVVALFERDALHHLPVSAWDLRHAVDAFRYMREAKHVGKIVLNVPQP
                     PEPDGTVLITGGTGGLGALVARHLAAEHGARRLLLTSRRGLEAEGARDLVAALAELGC
                     EAQVAACDVADRAQLADLLAAIPAEHPLTAVVHAAGVLDDGVIDSLDGERLRQVMAPK
                     VDGALNLHELTEDARLSQFVLFSSVASILGTPGQGNYAAANSFLDGLAHHRRAEGLPA
                     TALAWGAWERVGGMVGGLSEADRMRGERLGITGLSDELGLTLLDTARGAAQPLLVPVA
                     LDSGALRAQAKAGLLPPVLQGLVRTPVRRSSEATGSLATRLAAAPEAEWPAIVLDLVQ
                     SHVGAVLGHGSGDAIDPGRAFKELGFDSLSAVELRNRLIQATGLKLPTTLIFDHASPA
                     AVAEFLRSKVAGADRPKPTARRAPASTDEPIAIVGMSARFPGGVHSSGDLWRLVESGS
                     DAIGGLPDDRGWDLERLYDPDPDHPGTTYSREGGFLYDAGEFDADFFSISPREALAMD
                     PQQRLLLEGAWEALEHAGIVPASLRGSQTGVFVGVSSANYGIGPGTPPDVEGFRLTGS
                     TTSVASGRLAYTFGLEGPAVSVDTACSSSLVAMHWACQALRRGECEMALAGGVTVLAT
                     PGLFIEFSRQRGLSRDGRCKAFGAAADGTGWSEGAGILVLERLSYARRNGHRILAVIR
                     GSAVNQD"
BASE COUNT          440 a         1156 c         1298 g          392 t
ORIGIN      
        1 gtcctggttg acggcgctgc cgcggatgac ggccaggatc cggtggccgt tgcggcgggc
       61 gtaggacagc cgctccagga ccaggatgcc ggcgccctcg gaccagccgg tgccgtcagc
      121 ggcggcgccg aacgccttgc agcgcccgtc gcgcgagagc ccgcgctggc gtgagaactc
      181 gatgaacagg cccggggtgg cgagcaccgt gacgccgccg gccagcgcca tctcgcactc
      241 gccgcggcgc agtgcctggc acgcccagtg catggccacg agcgaggacg agcacgccgt
      301 gtccaccgag acggcgggcc cctccagccc gaacgtgtac gcgagccggc cggaggcgac
      361 gctggtcgtg ctgccggtca ggcggaagcc ctcgacgtcc ggaggcgtcc cgggaccgat
      421 gccgtagttc gcggacgaga cgccgacgaa gactcccgtc tgcgatcccc tgaggctcgc
      481 gggcacgatc ccggcgtgct ccaacgcctc ccacgctccc tccaggagca gccgctgctg
      541 gggatccatc gccagcgcct cgcgcgggga gatcgagaag aagtcggcgt cgaactcccc
      601 ggcgtcgtag aggaatccgc cctcgcgcga gtaggtggtg ccggggtggt cggggtcggg
      661 gtcgtagagg cgctcgaggt cccagccgcg gtcgtcggga agcccgccga tcgcgtccga
      721 gcccgattcg acgagccgcc agaggtcgcc ggacgagtgc accccaccgg ggaagcgcgc
      781 gctcatcccg acgatcgcta tcggctcgtc cgtggacgcc ggcgcgcgcc tggccgtcgg
      841 cttcggacgg tccgcgcccg cgaccttgga gcgcaggaac tcggccaccg ccgcggggct
      901 ggcgtggtcg aagatgagcg tggtcggcag cttcagtccg gtcgcctgga tcaggcggtt
      961 gcgcagctcg actgcgctaa gcgagtcgaa gcccagctcc ttgaaggcgc gccccgggtc
     1021 gatggcgtcg cccgagccgt gcccgagcac ggcgccgacg tggctctgca cgaggtcgag
     1081 cacgatcgcc ggccactcgg cctcgggcgc ggccgcgagc ctcgtcgcca gcgagcccgt
     1141 ggcctcggag gagcggcgga cgggcgtgcg caccagtccc tggaggaccg gcggcagcag
     1201 cccggccttg gcctgcgcgc ggagggcgcc gctgtcgagg gcgacgggca ccagcagcgg
     1261 ctgcgcggcg ccgcgggccg tgtcgagcag ggtcagtccc agctcgtcgg agagcccggt
     1321 gatgccgagg cgctcgccgc gcatgcgatc ggcctcgctg aggccgccga ccatgccgcc
     1381 gactcgctcc caggctcccc acgcgagcgc cgtggcgggc agcccctcgg cgcggcggtg
     1441 gtgggcgagg ccgtcgagga acgagttggc cgcggcgtag ttgccctgcc cgggggtgcc
     1501 gaggatggag gcgaccgacg agaacaggac gaactgcgag agacgcgcgt cctctgtgag
     1561 ctcgtgcagg ttgagggcgc cgtcgacctt cggggccatc acctgcctga gccgctcgcc
     1621 gtcgagcgag tcgatcacgc cgtcgtcgag cacgcccgcg gcgtgcacca ccgcggtcag
     1681 cggatgctcg gcggggatcg cggcgagcag gtcggcgagc tgcgcgcggt cggcgacgtc
     1741 gcacgcggcg acctgcgctt cgcagcccag ctcggccagc gcggcgacga ggtcccgggc
     1801 gccctcggcc tcgaggcccc gccggcttgt gagcagcagc cgccgcgcgc cgtgctcggc
     1861 ggccaggtga cgcgccacga gcgcacccag cccgccggtg ccgccggtga tcaggacggt
     1921 gccgtcgggc tccggcggct gcgggacgtt cagcacgatc ttgcccacgt gcttcgcctc
     1981 gcgcatgtag cggaaggcat ccacggcgtg gcgcaggtcc caggccgaga cgggcaggtg
     2041 atggagcgca tcgcgctcga agagcgccac gacctcgagc agcatcgcct ggatgcggtc
     2101 gggcccggcc tcggccaggt cgaacgctcg gtagcgaacg cccgggtgct gcgccgcgac
     2161 ctcgtcgggc tcgcggatgt cggccttgcc gatctcgacg aagcgcccgc cctgcggcag
     2221 gagatcgagc gaggcgtcga cgaactcgcg cgcaagggcg tcgaggacga cgtccatgcc
     2281 ggcgccctcc gtcgcgtcga ggaagcggtc gcggaagtcg aggtcgcggg aggaggcgat
     2341 gtggtcgtcg ggcacgccga gcccgcggac cgtgtcccac ttggccgggc tcgccgtggc
     2401 gtagacctcc gcgccgaggt gcttggcgat ctgcaccgca gccatgccaa cgccgccggc
     2461 cgccgcgtgg atcaggatcc gctcgccctg cttcaggccg gcgaggtcga cgagcgcgta
     2521 gtaggcggtc gcgaatacgg acgggaccga cgcggcctcg gcgaacgacc aggcgtcggg
     2581 gatcggcacc agcatgtggt ggtcggtcac gccatagggg ccgaacggct cgcggacgat
     2641 gcccatgacg cggtcgcccg gctggaggtg ctcgacgtcg gcggcggtct cgaccacgac
     2701 gccggcgccc tcgctgccga gcggagcctc gccgggatac atgccgaggg caatgaggac
     2761 gtcgcggaag ttgaggccgc cggcgcgcat cgcgacgcgc acgtggccct cggcgagcgg
     2821 cgcatcggcg gcgtccgacg ggacgagcgt gaggtcctcg agcgagccgg tgcgctcgat
     2881 gccgagccgc caggcgccgg cggtgggcgg gatgagcccg ctcgcccgcg ccagccgcgg
     2941 ggcgagcgca cggccctcgc gcagggcgag ctccggctcc tcggcggcca gcgcgccggc
     3001 gagcgcggag gccgaggcgt cggcgccgtc gaggtcgatc acgccgaagc gtcccgggtt
     3061 ctcggagtgg gcgctgcgca cgaggccgag cagggcggcc tgggcgaggt tcggcgactc
     3121 gacgccggag gtcgccaggg cgttgtcggt caggagcacg aggcgggact cggcgagcgg
     3181 ctcggcggcg agccaggact ggagcagcgc gagcgtgcgc gcggtgaggt cgtggaccga
     3241 gccggcgagg ctcgcggagg acccgtcgct ggtgggcgcg tcgatg
//
LOCUS       DASVPL010000056         2560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_359974, whole genome shotgun sequence.
ACCESSION   DASVPL010000056 DASVPL010000000
VERSION     DASVPL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359974"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="VF517_02920"
     CDS             complement(<1..1711)
                     /locus_tag="VF517_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX8051916.1"
                     /translation="MADPAYSETSLAEVLRNRAESTPDDTAFIFLEEDGSEQPATYGE
                     LHSEARKVAAVIDAQNSSPGGPVLLLFAPGLDYVAALFGCFYAGAPAVPAFPPDPFRI
                     SRTLPRLLAILEDSAADLVLTTEPLRESLEEWLRSGGSSSRVVATDVPHGGAENPAAG
                     DPPSDALAMLQYTSGSTRSPSGVMLAHEQLLSNAARMQVAFEFSERDVATIWLPPYHD
                     MGLIGGIIQSVYSGMRCVLMSPLTFLKRPITWLSALSRHRATVSGAPNFAYDLCVRRT
                     KPEEREGLDLSNLELTFSGSEPVSAATLTEFSRTFAPYGFRHEAFLPAYGLAEATLMV
                     SGTARLAGPRLFTADRDQLERGCVVESASGGDDARVLVSCGAPPIDHTVAIVDPSSLQ
                     KCPEGRVGEIWFSGPNVARGYWRKPEETRETFHATTSDTGEGPFLRTGDLGFLLDGQV
                     FVSGRLKDVIVVNGRNYHPVDIEKGCEAAVPGVRRGCGAVFAMESPDGREGIGVVYEV
                     GAGNGDHAETLDAVRRAISMETSMKPEAVALVAPRTIPKTSSGKIQRYLCRERLLADE
                     LDTV"
     gene            complement(1704..>2560)
                     /locus_tag="VF517_02925"
     CDS             complement(1704..>2560)
                     /locus_tag="VF517_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051917.1"
                     /translation="ALSERVGCAEWVVEGILDPLVDAELVRRDADGYATVAGLEPVLD
                     DSARRAAVEAELRSALAGPPGSLGGDDPPAAAYDGTAFGLAEALFERHLPELEGLDDL
                     LRGGSRRVLVIGPGSAELAIELCRWLAGVHIVGLEREPAALSQGMADVRAAGLAERVE
                     VVGDLAADAPFALAVVPVAFQPRAVVERELAALRDAVEPSGWVLSVAPNQPRDPLGAA
                     VRRLRTARSGGDPGLGEAELEELLGGVGFPVTRVLPPDGQFGLRIVAARNLPTRDDGD
                     GSGGGGNG"
BASE COUNT          375 a          939 c          900 g          346 t
ORIGIN      
        1 cgacggtgtc cagctcgtcc gcgagcaagc gctccctgca caggtagcgc tggatctttc
       61 cgctcgaggt cttcgggatc gtcctcggcg caacgagcgc gacggcctcc ggcttcatgc
      121 tcgtctccat cgatatggcg cggcggacgg cgtccagcgt ctccgcatgg tcgccgttgc
      181 cggcgccgac ctcgtagacg accccgatgc cctcgcgacc gtccggggac tccatggcga
      241 acacggcccc gcagccccgc cgcacgcccg gtaccgcggc ctcacatccc ttctcgatgt
      301 ccacggggtg gtagttgcgt ccgttgacga cgatgacgtc cttcagccgg cccgacacga
      361 acacctggcc gtcgagcagg aagccgaggt cgcccgtcct caggaacggt ccctcgcccg
      421 tgtcggaggt cgtcgcgtgg aacgtctcgc gggtctcctc gggcttgcgc cagtaaccgc
      481 gcgcaacgtt ggggcccgag aaccagatct cgccgacgcg gccctcgggg cacttctgca
      541 gtgacgaggg atcgacgatc gcgaccgtgt ggtcgatcgg cggagctccg cagctcacga
      601 gcacgcgcgc gtcgtcgccg ccgctcgcgg actcgacgac gcagcctcgc tcgagctggt
      661 cccggtctgc ggtgaagagt cgcggtcccg cgagccgcgc ggtgccgctg accatcagtg
      721 tcgcctccgc gaggccgtag gcgggaagga acgcctcgtg ccggaacccg tacggggcga
      781 aggtgcgcga gaactccgtg agcgttgcgg cgctcaccgg ctccgacccg ctgaacgtga
      841 gttccaggtt gctcaggtcg agcccctcgc gttcctccgg cttcgtgcgc cgcacgcaca
      901 ggtcgtacgc gaagttgggg gcgccgctga ccgtggcccg gtggcgtgac agtgcactca
      961 accaggtgat cggacgcttc aggaacgtga gcggcgacat cagcacgcag cgcatccccg
     1021 agtagaccga ctggatgatg ccgccgatga gccccatgtc gtggtacggc ggcagccaga
     1081 tcgtggcgac gtctcgttcc gagaactcga acgcgacctg catccgggcg gcgttgctca
     1141 gcagctgctc gtgcgccagc atgaccccgg agggcgaccg ggtggagccg gacgtgtact
     1201 ggagcatcgc gagcgcgtcg ctgggcggat cgcccgcggc cgggttctcc gccccgccgt
     1261 gggggacgtc ggttgccacg acgcgagacg aggagccgcc ggatcggagc cactcctcga
     1321 ggctttcgcg cagaggctcc gtagtcagga cgaggtcggc cgccgagtcc tcgagaatcg
     1381 cgagcagccg tggcagcgtc cggctgatgc ggaacgggtc cggcggaaag gccggaaccg
     1441 ccggcgctcc ggcatagaag cacccgaaga gggccgcgac atagtccagc cccggcgcga
     1501 acagcaacag caccgggccg ccgggactgc tgttctgcgc atcgatcacc gccgccacct
     1561 tgcgtgcttc gctgtggagc tcgccgtacg tcgccggttg ctcggagccg tcctcctcca
     1621 agaagatgaa cgccgtgtcg tccggagtgg actcggcacg gttgcggagc acctcggcca
     1681 agctcgtctc ggagtaggcg ggatcagcca ttgccgccac cgcccgaacc gtcgccgtca
     1741 tcgcgcgtgg gcagattgcg cgccgccacg atgcgcaggc cgaactggcc gtcagggggg
     1801 agcacccgtg tcaccgggaa gcccacgcct ccgagaagct cctcgagctc cgcctcgccg
     1861 aggcccggat cgccgccgga tcgtgccgtg cggaggcggc gcacggctgc gccgagcggg
     1921 tcgcgaggtt ggttcggcgc cacggacagc acccagccgc tcggctcgac ggcgtcgcgc
     1981 agcgcggcga gctcgcgctc gaccaccgcg cggggctgga aggccaccgg cacgaccgcg
     2041 agcgcgaacg gcgcgtctgc ggcgaggtcg cccacgacct ccacgcgctc ggcgagcccg
     2101 gcggcgcgga cgtccgccat cccctgcgac agcgccgcgg gctcgcgctc caacccgacg
     2161 atgtgcacgc cggcgagcca gcgacacagc tcgatggcga gctccgccga ccccggaccg
     2221 atcacaagca cgcgccgcga gccgccccgg aggaggtcgt cgagcccctc cagctccggc
     2281 aggtggcgtt cgaacagcgc ctcggcgagg ccgaaggcgg tgccgtcgta tgccgccgcg
     2341 gggggatcgt cgccaccgag cgaccccggc gggcccgcca gcgccgagcg aagctccgcc
     2401 tcgaccgcgg cgcgccgcgc gctgtcgtcg agcaccggct cgagcccggc gacggtcgcg
     2461 tacccgtcgg cgtcgcgccg gaccagttcc gcgtccacga gggggtcgag aatcccttcc
     2521 accacccatt cggcgcagcc gacgcgttcg ctcagggccg
//
LOCUS       DASVPL010000057         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_163361, whole genome shotgun sequence.
ACCESSION   DASVPL010000057 DASVPL010000000
VERSION     DASVPL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163361"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..343
                     /locus_tag="VF517_02930"
     CDS             68..343
                     /locus_tag="VF517_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051918.1"
                     /translation="MSAEHPDGGGRREAAGPTARPRYEEAERRMRAIVADAGLPEPDD
                     VEYEDTSIFLYWHEQKLVVEIADIPVTPPGPAEEPPATGGDAACRDA"
     gene            complement(449..521)
                     /locus_tag="VF517_02935"
     tRNA            complement(449..521)
                     /locus_tag="VF517_02935"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(486..488),aa:Ala,seq:ggc)
     gene            complement(1004..1354)
                     /gene="rsfS"
                     /locus_tag="VF517_02940"
     CDS             complement(1004..1354)
                     /gene="rsfS"
                     /locus_tag="VF517_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX8051919.1"
                     /translation="MTVASPDRLAERIAAVASDRKARDIRVLDVASLVGYTDYFLICS
                     GGTERQTKAIHDAIHEALKGDGVYPARTEGAREARWILMDYGDCVVHIFTPEARAFYR
                     LEQLWGEAPAHSVS"
     gene            complement(1351..>1701)
                     /locus_tag="VF517_02945"
     CDS             complement(1351..>1701)
                     /locus_tag="VF517_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051920.1"
                     /translation="LEALAKERPDDELWWILGGDQAAKLRSWRDPDRVLELATIAAAE
                     RGAWRRPGIFVEASHLRNSGRLVFFDMPPVGLSSTMIRRRVARGQPVRYLVPDGVAGL
                     IEERGLYRAGVAAR"
BASE COUNT          250 a          626 c          593 g          232 t
ORIGIN      
        1 gcaggtccgt cgccgatttt tcacgcctgt aacccacgtg taacgcggcg tcggcgaagc
       61 tcgcggcatg agcgcggagc atcccgatgg cggcggccgg cgcgaggcgg ccgggcccac
      121 ggcgcgtccc cgatatgagg aggcggagcg ccggatgcgc gcgatcgtcg ccgacgcggg
      181 ccttcccgag cctgacgacg tcgagtacga ggacaccagc atcttcctgt actggcacga
      241 gcagaagctc gtggtcgaga tcgccgacat cccggtcacg ccgccaggcc cagccgagga
      301 gccgccggcg acaggcggag atgcggcttg ccgcgacgcg tgacgacgat cggctcgccc
      361 gcggcggcgc gctccatgta ccagccgaag cggttgcgga actcgtgggc cccgatgacg
      421 gtccccgcgg cgcggtcgag cgaaggagtg gagctagtcg gactcgaacc gacgaccccc
      481 tcgctgccag cgaggtgctc ttccagctga gctatagccc cgagggtgta ctcgtcggcg
      541 aagtgtagcc ctgcgcgctg accgtttcgc gcaggcgcga gccggaggct gaccgccgtc
      601 cggccgccca cgagcgagat cgggagcgcg tagcagcggt ccagctccgc gcagtaggcc
      661 gcgacgtagt cgacctcctc ggacgtgtac gtcgtggtgg cgaatccgtc gcgcgtgcgc
      721 cggtgcgtgc ggatcttgac ctcgacgacg tcgccccgca ggaccgccca cttgcactgc
      781 actcgcgcca cgcggcggcc cagatcgaag gccaggtcgt agcgcagggc ctcggtcatc
      841 gggcgcagca ccggtatccc cagcttcgcc gcctccgccg cgatcgccag ctccgcgatg
      901 ccgcccttct cgcttgcgtt catgacgcga acactagttc gctcaacgga cggaaccgcc
      961 gcagaaatcc cctgcaaatc cgcccttcgg ggggtcggat cccctaactg acggagtggg
     1021 cgggagcctc gccccagagc tgctcgaggc ggtagaaggc gcgcgcctcg ggcgtgaaga
     1081 tgtggaccac gcagtcgccg tagtccatga ggatccagcg cgcctcgcgc gctccctccg
     1141 tgcgcgccgg atacacgccg tcccccttca gcgcctcgtg gatcgcgtcg tggatcgcct
     1201 tcgtctggcg ctcggtgccg ccgctgcaga tcaggaagta gtccgtgtag cccacgagcg
     1261 acgcgacgtc gagcacgcgg atgtcccggg ccttgcggtc cgaggcgacc gcggcgatgc
     1321 gctcggccag gcggtcgggc gacgcgaccg tcatcgcgcg gccacccccg cgcgatacag
     1381 gccgcgctcc tcgatcagcc ccgcgacccc gtccggcacg aggtacctga cgggctggcc
     1441 gcgcgcgacg cggcggcgga tcatggtgga cgacagcccc accggcggca tgtcgaagaa
     1501 cacgagccgc ccgctgttgc gcaggtgcga ggcctccacg aagatccccg gccgccgcca
     1561 cgcgccgcgc tcggccgccg cgatcgtcgc gagctccagc acgcggtcgg ggtcgcgcca
     1621 cgagcgcagc ttggccgcct ggtcgccgcc gaggatccac cagagctcgt cgtccggccg
     1681 ctccttcgcg agcgcctcga g
//
LOCUS       DASVPL010000058        13122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_360240, whole genome shotgun sequence.
ACCESSION   DASVPL010000058 DASVPL010000000
VERSION     DASVPL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360240"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VF517_02950"
     CDS             complement(<1..1078)
                     /locus_tag="VF517_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8051921.1"
                     /translation="MVAVGALAAFTAWSYLTIAWADQRADAWDGSNRTALYAAVFALF
                     ALWRIRARAAVAIVGAFALGVAAIGVVELVSVISSADPTGHFIDGRLAEPVEYANANA
                     ALWSSAFWPCLVLGSRRETGPLLRALFVGAAVLLGGLALMGQSRGWLFALPIVALLFL
                     VITPQRVRTTLTLALVLGAVGVCVPAILDVYDESGPPLAGATDDAGTAILGAAAVAAA
                     LAGMGAFADRAQRRSRRVDQAAGWVLGAAAAVAIVAGTALYVAERGSPFTDISDAWQE
                     FKTTGTPGGGSNRLVGRLGSNRYDFWRVAWDEFEAAPVGGIGADNFQAAYLERSESGE
                     QPRYPHSVELRTISQTGLVGTALLV"
     gene            1579..1875
                     /locus_tag="VF517_02955"
     CDS             1579..1875
                     /locus_tag="VF517_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051922.1"
                     /translation="MRFRNLIVGAIVAASMLIAAPAFAQTASEGYSKPAGSIQERIST
                     ENPPNDPPEEITETRAARSELPFTGLDVALIVGAGGLLLAMGFGMRRLSRTEVA"
     gene            2115..3515
                     /locus_tag="VF517_02960"
     CDS             2115..3515
                     /locus_tag="VF517_02960"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8051923.1"
                     /translation="MLEGKSWTLTALCVDGFMLLLAVAAALFGATAADVHATTWPVFL
                     FPPIVMGLLAIRGVYRPKITVQFLDEVGHVVGACSVAAMSIIAVSIFTAGGGEQAELL
                     ARVWLFGMVYLAGSHYVLDITQRRARIERAIAVPTLIFGAGKIGAQVERRLDEKPELG
                     LRPVGYVDPYPPSDEQVPGRRVPILGAPEDLEGIVARTGAKHVVMAFMSSRGSDATLV
                     PVVRKCDELGLDVSLVPRLFESINVRVKLEHIGGMPLYRLRAVNPKGWQFAIKHALDR
                     VMAAVLIVLLAPLLLAALAAVRISSPGPVFFRQRRVGRDGKPFDLLKFRSMRLTEARP
                     KDNVKMLLPDDTAPGGVEGADRRTAIGKLIRRLSIDELPQLFNVLKGEMSIVGPRPER
                     PEFVELFERRVTRYEDRHRVKAGITGWAQVHGLRGKTSLSDRIEWDNYYIENWSLWLD
                     MKILLLTVAAVFQPAE"
     gene            3529..4314
                     /locus_tag="VF517_02965"
     CDS             3529..4314
                     /locus_tag="VF517_02965"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX8051924.1"
                     /translation="MIDLHCHILPGLDDGPVNLDFSVAMARAAAENGIQVMVATPHIR
                     ADYDVDPGAIADLVEGLNAAVEEDDTQLLVLAGGEVSLQKATELEDDVLRTVCLGSSD
                     YLLLESPYKAIDIDIEGIVREVQGRGFKPLLAHPERCPIFQHDPDRLQRLVNMGVLCS
                     ITASSLAGGFGSTVRRFTLELVHDGLIHDIASDAHDHLHRPPDLLAGFESAEGEIPGI
                     QSQAPWFTVTAPVAILAGRPLPARPDPPPRPPTSRWRRLVNRG"
     gene            4415..5839
                     /locus_tag="VF517_02970"
     CDS             4415..5839
                     /locus_tag="VF517_02970"
                     /inference="COORDINATES: protein motif:HMM:NF020069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8051925.1"
                     /translation="MSLRRPLLAGIAAAIALAVPASASAATCDKVAAPGGSNTAAGTE
                     AAPYASFQKLVDSLAPGQAGCLRGGTYREENVTVGRGGTADARITVRSYPGERALHIG
                     RLYVTDRANFLTFEQLDLNGSGGPKNCNPDGCGLLPSPTVNGDDVVFQDNDVTNDHTS
                     ICFNVGNPGWGAALRVTIQRNRIHNCGRMPASNHDHGIYLVETQDVQILDNVIYDNAD
                     RGIQLYPNADRTTIRSNILDGNGQGIIFSGVGGQTSDNNVAEHNIVTNARLRYNIESY
                     YPDGTGTGNVARNNCLYGGERGNVSAQDGFVAISNLVADPQYVDRAAKDFRLKAGSPC
                     AAILAGADVPATPAAPTSPTSPTSPTSTTPTGTDQGTDEPAGKPGEVILENVSVKRSK
                     RTGRLRLRVSGRLTGWGADKLKVQVRRGNGWKTLGTIRGVRKIFRATVRPPSANLRKS
                     NRLAVRVVIPGVDQSNTVLARTRR"
     gene            complement(5852..6625)
                     /locus_tag="VF517_02975"
     CDS             complement(5852..6625)
                     /locus_tag="VF517_02975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8051926.1"
                     /translation="MSDLVVLCYHAVSPDWPAALSLRPEVLERQLTFLSRRGYRGIPA
                     SEALESPSGRAVAITFDDAYRSVLEIAKPILDRLDMRASVYVPTDWPDREGPMSWPGV
                     AHWVGGPHESELSCMSWDELNGLADAGWEIGSHTCSHPRLAELATEEEIARELRESRE
                     VCQERLGRPCRSIAYPYGSYDHRVVDAAGDAGYDFGLTLPHGLHGPEPLAWPRIGIYH
                     ADRTYRWRLKLSPTIRRARALRAWEVASGLKRRLRGSES"
     gene            complement(6622..7590)
                     /locus_tag="VF517_02980"
     CDS             complement(6622..7590)
                     /locus_tag="VF517_02980"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HEX8051927.1"
                     /translation="MATDLAPGRERGRLERFLSRSFGAPYGHHVDHEKVGGYPIDFRA
                     KANQLKMWPGFHERPGSELWVGAIQRGLGCYERFLCDEGEQWLESARETADLLVRAQR
                     EDGSWVHTFDYPHTFALSPPWTSGMAQGEGASLLVRLHDQTGEERYAEAALRAVERLY
                     VASSDGGAAAELNGTLVPEEYPTQPRSLVLNGLMFGLWGLRDVAVATGDERARAAFDD
                     GVAALIGSIGHWDTGNWSRYDLFPHRVANVASMAYHHLHITQLDATGRLARRPELVAV
                     AWRWAGYADRREYEALAFARKALFRLAEPRNARVARLLPWVRGGTS"
     gene            7660..8814
                     /locus_tag="VF517_02985"
     CDS             7660..8814
                     /locus_tag="VF517_02985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8051928.1"
                     /translation="MPDAPNSERRLRVLTLVDHLGASGGGERLAMEISKRLDRDRFEA
                     IHCASRWHPEASGEAMRAAADELRAAGVRVIGLGRGSTAAVWSWAPLVRLLRRERVDV
                     LHSHKFGSNVWGVVLGGLAGVPAVVAHEHTWSYEGQPLRRFLDRELIARRSDAFVAVS
                     HEDRRRMIEVERIDPEDVVFVPNGIDALPDGDGARVRSELGIEPGAPVIGAVAVLRPQ
                     KALHLLVRAAAELVREHPGLRVLIAGDGPEREALEALVRELGLEGVVTLLGLRRDVPD
                     LLAALDVAVVSSDFEGTPLAVLEYMDAGLPVVATRVGGLSDLIEHGRHGLLVDPGDAG
                     VLAQAIGTLLRDPERARELGARGRERRRAEFSIDRTVRTLEDMYVNLAGR"
     gene            8811..9854
                     /locus_tag="VF517_02990"
     CDS             8811..9854
                     /locus_tag="VF517_02990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8051929.1"
                     /translation="MIEEVRELEPLREDWQRLAEAAGSPFSTYEWAETWWRHYGAGRE
                     LHLLRCRDDAGRAFAILPLFLARSRPVRILRLVGQGAADQLMPICAPQDADAAAAALH
                     EALRDGLAGWQALIADRLPGEEWAERLGGRALDRTPSPVLEAGGASFDDWLATRSKNF
                     RDQVKRRERKLAKSHELAFRLTDDPARLDEDLGTLIRLHRERWGDASGSFEGGREEFH
                     RDFARVALDRGWLRLWTLELDGRPAASWLGYRFGGDEWYYQAGRDPELEREAVGFVLM
                     AHTVRAALDDGVARYRLLLGGEGYKDRFANADHGLHTTMLGRGVLGQVLAVGAETAAT
                     RPALRARLKRFAR"
     gene            complement(9835..11226)
                     /locus_tag="VF517_02995"
     CDS             complement(9835..11226)
                     /locus_tag="VF517_02995"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8051930.1"
                     /translation="MAARLNTGSHLAYQALLGTIAVGLGLLAGLDPRLAVAAAIGLAF
                     VVLVLGDLTFGLCLFAIVSFLDLLPTLGGSLLSFSKIVGLLIAVSWLAKVSTSNDARN
                     DFLAAHPVFSYVLGLFLGWTALSLTWAEDAAAGRTSVMRFALNLILFLIVYTAVRTPK
                     QFVLTVGAYVTGAAVAAAYGLLFPPQDTAYYDVSRVGGTLGDPNELAAVLVPGMILGA
                     ALAFSLKRFPMVRIAAVVASILCAAGVFLTLSRGGLIAAVVALSASAFVAGRWRIQAI
                     TLAVVAVFGGLFYFGFVASQDAVNRVTEVEGGSGRTDLWKVGLRMVQAKPVSGVGVGN
                     FQTASIHYLLQPGALKRDEFIVDNPKSAHNTYLHVLAELGFVGFALFAAIILFALLCI
                     LRAARIFDRIGRRDLELLARALLVAITGVLAADFFISEMFGKQLWLLLGLGPALLGVA
                     RTVQAERLSARSA"
     gene            complement(11242..12834)
                     /locus_tag="VF517_03000"
     CDS             complement(11242..12834)
                     /locus_tag="VF517_03000"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX8051931.1"
                     /translation="MTPPESSRSAEWLRPRVEQQGLQRYVNTIRERIKLIAIVTVLTT
                     LAAIAYVMLAPKVYESQSELLVTPVPADDPALSGLPIIRGSSDPTRDVETAARLVANR
                     QVAQAVRRKTPGLPEPLLEHVKAEPVAQSNIVAITAEAPSGKEAQQIANGFAESSIQV
                     QTQKLHAALDKSIANLEARVEALSGPDEAAGGGEDTSLRSQLARLQTLRGQDDPTLSI
                     QTRAADPDEAASPKKKLSLISGVLAGLILGIGGAFALQVLDPRLRREDQLRTLYGLPI
                     LGRIPRETKRTDSALAPERLSPGTLESYRTLRATLAASRRGMRIDEAPAVLVTSPSPS
                     EGKSTTAINLASSLALAGNSVILIEADLRRPAIGAALGVKGEYGTGSVLLGMAELEDA
                     LVETKAYGRYLRVLLADTSSGASGFMADQLFLPAAQNLVTDAKRIADYVIVDSPPLSE
                     VIDALPLAQQVDEVLVVVRLGRTQLTKLANLGELLARHDIEPVGFAVVGVPPQGDGYY
                     YTASGAEPARGKGRAAQGAGRG"
     gene            12918..>13122
                     /locus_tag="VF517_03005"
     CDS             12918..>13122
                     /locus_tag="VF517_03005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8051932.1"
                     /translation="MPHVDASVLVPVLNEERFIRDTVRAMQGQRFDGEVEFLFMDGRS
                     DDATRAILDEIAQEDPRVRVLDNP"
BASE COUNT         1952 a         4723 c         4714 g         1733 t
ORIGIN      
        1 ctaccagcag agcggtgccg accaggcccg tctgcgagat cgtgcgcagc tccacgctgt
       61 gcgggtagcg cggctgctcg ccgctctcgg agcgctcgag gtacgcggcc tggaagttgt
      121 ccgcgccgat gccgccgacc ggcgcggcct cgaactcgtc ccaggcgact cgccagaagt
      181 cgtagcggtt cgagccgagc cgcccgacca agcggttgga gccgccgccg ggcgtgccgg
      241 tggtcttgaa ctcctgccac gcgtcggaga tgtccgtgaa cggactcccg cgctcggcca
      301 cgtacagcgc ggtgccggcc acgatcgcca ccgccgcggc ggccccgagc acccatcccg
      361 ccgcctgatc gacgcggcgc gagcgccgct gcgcccgatc ggcgaaggcc cccatgccgg
      421 ccagggcggc cgccacggcc gcggcgccca ggatcgcggt gccggcgtcg tcggtggcgc
      481 cggcgagcgg gggcccggac tcgtcgtaga cgtcgaggat cgccggtaca cagacgccga
      541 ccgcgccgag cacgagcgcc agggtcagcg tcgtgcgcac gcgctgcggc gtgatcacca
      601 ggaacagcag cgccacgatc ggcagcgcga acagccagcc gcggctctgc cccatgagcg
      661 ccaggccgcc gagcagcacg gcagcgccca cgaacagcgc gcgcagcagc gggccggtct
      721 cacgccgcga tccgagcacc aggcagggcc agaacgccga cgaccagagc gccgcattgg
      781 cgttcgcgta ctcgaccggc tcggccaggc ggccgtcgat gaaatgcccc gtcggatcgg
      841 cggaggagat gacgctcacg agctcgacca cgccgatcgc ggcgaccccg agcgcgaagg
      901 cgccgactat cgccacggcc gcccgcgcgc gtatgcgcca caacgcgaac agcgcgaaga
      961 ccgccgcgta gagcgccgtc cggttcgagc cgtcccaggc gtcggcgcgc tggtcggccc
     1021 atgcaatcgt gaggtagctc cacgccgtga aggcggcgag cgcgccaacg gcgaccacta
     1081 cggggaccgg cgggcgcggc cccgtgggca gcgccagcag cccgaggagg agcagcgcga
     1141 ctaggaaaag ccctgctggg taccactgcc cagggaagta gccgcccttg acagcggaca
     1201 gaatgaagag gggaaccatc gccaacgcgc caagaacggc ggcaggagat aacgcgatcg
     1261 gcctacgcag cgtttctgca acggtgcgca ggggcggcac tcgcaagatt ctagggcggg
     1321 taccgcacgg atcgggccgc gcatcctgcg caaaggctga tccgaaaaac cgtgccgccc
     1381 gaacggttcg tactatccac ggaactgcag tgcgtacccc aacaaccgat atcttgcaga
     1441 acaggctctt tgcggctact gtttttcagc cgcggggagg ggtaccagag gtactcacga
     1501 aagaccgggg ccatggagcg gttctggttt acgaggctca cggcaaggaa gccaaatcaa
     1561 atggtaggga gaccacctat gcgttttagg aacctgattg tcggcgcgat cgtcgccgcc
     1621 agcatgctca tagccgcccc cgcgttcgcg cagacggcat cagagggcta ctcgaagccc
     1681 gcaggcagca tccaggagcg gatctccacg gagaacccgc cgaacgaccc gcccgaggag
     1741 atcaccgaga cccgtgccgc gcgcagcgag ctgccgttca ccgggctcga cgtcgcgctg
     1801 atcgtcggcg ccggcggact cctgctggcc atgggcttcg ggatgcgtcg cctcagccgt
     1861 accgaggtag cctgacctga acccggtttc ccgcgccggg ctaacaaaaa cgcgggcccg
     1921 ccccgataca gggcgtggag agtgatcgct tgcctggcca aaccagacgg gacgacctag
     1981 cgctcgccga ttcggcgagg gtctcggaca cgtcccctgg cacggccggg gccgcgatcg
     2041 acccgatcga agactcagcc gcagcgacgc cgccggagca tctccctaca gcccggcggc
     2101 gttcgcgcgc ctggatgctc gagggcaagt cgtggacgct gacggccctc tgcgtcgacg
     2161 ggttcatgct tctgctggcc gtcgccgcgg cgctcttcgg cgcgactgcc gcggacgtgc
     2221 acgccacgac ctggcccgtc ttcctgttcc cgcccatcgt gatggggctg ctcgcgatcc
     2281 gcggggtcta tcgccccaag atcaccgtcc agttcctcga cgaggtcggg cacgtggtcg
     2341 gcgcgtgctc ggtcgcggcc atgtcgatca tcgccgtgtc gatcttcacc gcgggcggcg
     2401 gcgagcaggc cgagctcctc gcccgcgtgt ggctcttcgg gatggtctac ctggccggca
     2461 gccactacgt cctcgacatc acgcagcggc gtgcccgcat cgagcgcgcg atcgccgtgc
     2521 ccaccctgat cttcggcgcc ggcaagatcg gcgcccaggt cgagcgccgc ctcgacgaga
     2581 agcccgagct cgggctgcgg ccggtcggct acgtcgatcc ctacccgccg tcggacgagc
     2641 aggtgcccgg ccggcgcgtg ccgatcctgg gcgctcccga ggacctcgag ggcatcgtcg
     2701 cgcggacggg cgccaagcac gtcgtcatgg cgttcatgtc ctcacgcggc tccgacgcca
     2761 cgctcgtccc ggtcgtgcgc aagtgcgacg agctggggct cgacgtctcg ctcgtgccgc
     2821 gcctgttcga gagcatcaac gtgcgcgtca agctcgagca catcggcggg atgccgctct
     2881 acaggctgcg cgcggtcaac ccgaagggct ggcagttcgc catcaagcac gcgctcgacc
     2941 gcgtgatggc cgccgtgctg atcgtgctgc tcgcgccgct gctgctggcc gccctggccg
     3001 ccgtacggat ctcctccccc ggccccgtct tcttccgcca gcgccgcgtg ggccgcgacg
     3061 gcaagccgtt cgacctcctg aagttccgct cgatgcgact caccgaggcg cgcccgaagg
     3121 acaacgtgaa gatgctgctg cccgacgaca ccgcgccggg cggggtcgag ggcgccgacc
     3181 ggcgcaccgc gatcggcaag ctgatccgcc gcctgtcgat cgacgagctg ccccagctct
     3241 tcaacgtgct gaagggcgag atgagcatcg tggggccgcg tcccgagcgg cccgagttcg
     3301 tcgagctgtt cgagcggcgc gtgacgcgct acgaggaccg ccaccgcgtg aaggccggga
     3361 tcaccggctg ggcgcaggtg cacgggctgc gcggcaagac gtcgctctcg gaccggatcg
     3421 agtgggacaa ctactacatc gagaactggt cgctctggct cgacatgaag atcctgctgc
     3481 tcacggtcgc ggcggtcttc cagcccgccg agtagcgtcc gcacggacgt gatcgacctc
     3541 cactgccaca tcctgccggg gctcgacgac ggcccggtca acctcgactt cagcgtggcc
     3601 atggcccgcg ccgccgccga gaacgggatc caggtgatgg tcgccacgcc gcacatccgc
     3661 gcggactacg acgtggaccc gggagccatc gcggacctcg tggaggggct caacgcggcc
     3721 gtggaggagg acgacacgca gctgctcgtc ctggccggcg gcgaggtctc gctccagaag
     3781 gccaccgagc tcgaggacga cgtgctgcgc accgtctgcc tcggctcgag cgactacctg
     3841 ctgctcgaaa gcccctacaa ggcgatcgac atcgacatcg aggggatcgt ccgcgaggtg
     3901 cagggacgcg gcttcaagcc gctgctcgcg cacccggagc gctgcccgat cttccagcac
     3961 gaccccgacc gcctccagcg gctcgtgaac atgggggtgc tgtgctcgat cacggcgtcc
     4021 tcgctggccg gcggcttcgg ctcgaccgtt cggcgcttca cgctcgagct ggtccacgac
     4081 gggctgatcc acgacatcgc ctcggacgcg cacgaccacc tgcaccgccc gccggacctc
     4141 ctggcgggct tcgagtcggc cgagggcgag atcccgggca tccagagcca ggcgccctgg
     4201 ttcaccgtca cggcaccggt ggcgatcctc gccggacggc cgctgccggc gcgcccggac
     4261 ccgccgccac gcccgccgac ctcgcgctgg cgccggctcg tgaaccgcgg ctagatacca
     4321 cgtccaaggg tgtgttcagg cgccacctgg acatgtgtcc tagcaaggcg tcataaccct
     4381 ctgaaactcc ggcgcgcggc ccgatgcagg cgtcatgtcc ctccgccgcc ctctcctcgc
     4441 aggcatcgcc gccgccatcg cactcgccgt ccccgccagc gcctccgccg ccacctgtga
     4501 caaggtcgcc gctcccggtg gctcgaacac cgccgccggg accgaggccg ctccctacgc
     4561 cagcttccag aagctcgtcg actcgctcgc tcccggccag gccggctgcc tgcgcggcgg
     4621 cacctaccgt gaggagaacg tcacggtcgg ccgcggcggc acggcagacg cccgcatcac
     4681 cgtccgcagc tacccgggcg agcgcgccct ccacatcggc cgcctctacg tgaccgaccg
     4741 ggccaacttc ctcacgttcg agcagctcga cctgaacggc tcgggcggcc cgaagaactg
     4801 caaccccgac ggctgcgggc tgctgccgag cccgacggtc aacggcgacg acgtcgtctt
     4861 ccaggacaac gacgtgacga acgaccacac gtcgatctgc ttcaacgtcg gcaaccccgg
     4921 ctggggcgcc gcgctccggg tcaccatcca gcgcaaccgc atccacaact gcggccgcat
     4981 gcccgccagc aaccacgacc acggcatcta cctggtcgag acccaggacg tccagatcct
     5041 cgacaacgtg atctacgaca acgccgaccg cgggatccag ctctacccga acgccgaccg
     5101 gaccaccatc cgcagcaaca tcctcgacgg caacggccag ggcatcatct tctcgggcgt
     5161 cggcggccag acctcggaca acaacgtcgc ggagcacaac atcgttacca acgccaggct
     5221 ccgctacaac atcgagtcct actaccccga cggcaccggc accggcaacg tcgcccgcaa
     5281 caactgcctc tacggcggcg agcgcggcaa cgtcagcgcg caggacggct tcgtcgccat
     5341 cagcaacctg gtcgcggacc cgcagtacgt cgatcgcgcc gccaaggact tccggctgaa
     5401 ggccggcagc ccgtgcgcgg cgatcctcgc cggcgccgac gtgcccgcca ccccggcggc
     5461 ccccacgtcg cccacgtcgc ccacgtcgcc cacgtccacc acgcccacgg gtaccgacca
     5521 gggcaccgac gagccggccg gcaagcccgg cgaggtcatc ctcgagaacg tctcggtcaa
     5581 gcgcagcaag aggactggcc gcctgcgcct gcgcgtctcc ggccgcctga ccggctgggg
     5641 cgccgacaag ctgaaggtcc aggttcgccg cggcaacggc tggaagacgc tcggcacgat
     5701 ccgcggcgtg cgcaagatct tccgcgcgac cgtccggccg ccctccgcca acctccgcaa
     5761 gtcgaaccgc ctcgccgtcc gggtcgtcat ccccggcgtc gaccagtcga acacggtcct
     5821 cgcgcgcacc cggcgctaac gcgccgagcg cctagctctc cgagcccctc aggcgccgct
     5881 tgaggccgga ggccacctcc caggcacgca gcgcgcgcgc ccggcggatc gtcggggaga
     5941 gcttcaggcg ccagcggtag gtgcggtcgg cgtggtagat gccgatccgc ggccaggcca
     6001 gcggctccgg gccgtgcagg ccgtgcggca gggtgagccc gaagtcgtag ccggcgtccc
     6061 ccgcggcgtc gaccacgcgg tggtcgtagg agccgtaggg gtaggcgatc gagcggcacg
     6121 gccgcccgag gcgctcctgg cacacctcgc gcgactccct gagctcgcgg gcgatctcct
     6181 cctcggtcgc cagctcggcc agccgcgggt gcgagcacgt gtgcgagccg atctcccaac
     6241 ccgcgtcggc caggccgttc agctcgtccc agctcatgca ggagagctct gactcgtgcg
     6301 ggccgccgac ccagtgcgcc acgcccggcc agctcatcgg tccctcgcgg tccggccagt
     6361 cggtaggcac gtagaccgac gcgcgcatgt cgagccggtc gaggatcggc ttagcgatct
     6421 cgagcacgga gcggtaggcg tcgtcgaagg tgatcgcgac cgcgcggccc gacggcgact
     6481 cgagcgcctc ggaggccggg atgccgcggt agccgcgtcg gctcaggaac gtgagctggc
     6541 gctcgaggac ctcgggccgc agcgacagcg ccgccggcca gtcggggctc acggcgtggt
     6601 agcagagcac gacgaggtcg ctcacgaggt gcctccgcgc acccacggca gcagccgcgc
     6661 cacgcgtgcg ttgcgcggct cggcgagccg gaacagcgcc ttgcgggcga acgcgagcgc
     6721 ctcgtactcg cggcggtcgg cgtacccggc ccagcgccac gcgaccgcga cgagctcggg
     6781 ccgccgcgcg agcctgccgg tggcgtcgag ctgggtgatg tggaggtggt ggtaggccat
     6841 gctcgcgacg ttcgcgaccc ggtgcgggaa caggtcgtag cgcgaccagt tgccggtgtc
     6901 ccagtggccg atcgagccga tcagcgcggc cacgccgtcg tcgaacgccg cccgggcgcg
     6961 ctcgtcgccg gtcgccacgg ccacgtcgcg caggccccag agcccgaaca tgagcccgtt
     7021 cagcaccagc gagcgcggct gggtcggata ctcctccggg acgagagtgc cgttcagctc
     7081 ggccgcggcg ccgccgtcgg aggaggccac gtagagccgc tccacagcac gcagggccgc
     7141 ctccgcgtag cgctcctcgc ccgtctggtc gtgcaggcgg accagcaggc tggcgccctc
     7201 accctgcgcc atcccggagg tccagggcgg gctcagggcg aacgtatggg gatagtcgaa
     7261 ggtgtgaacc caagatccgt cctcgcgctg cgcgcgaacc aggaggtccg ccgtctcgcg
     7321 cgcggactcg agccattgct cgccctcgtc gcagaggaag cgctcgtagc agccgagccc
     7381 gcgctggatc gcgcccaccc acagctccga gcccggccgc tcgtggaagc cgggccacat
     7441 cttgagctgg ttcgccttcg cgcggaagtc gatcgggtac ccgccgacct tctcgtggtc
     7501 gacgtgatgg ccgtaggggg cgccgaacga gcggctcagg aagcgctcga gccggccgcg
     7561 ctcccggccg ggtgcgaggt cggtggccac cgagcggggc cgggcccgct cgtcttcggg
     7621 aatcgcggtc atcgggtgaa taggtcaggc tattaagcgg tgccggacgc cccgaacagc
     7681 gagcggcggc tgcgagtcct cacgctggtc gaccatctcg gcgcctccgg cggcggcgag
     7741 cggctggcca tggagatctc gaagcggctc gaccgcgacc gcttcgaggc gatccactgc
     7801 gcgagccgct ggcaccccga ggcgtccggg gaggccatgc gcgcggccgc cgacgagctg
     7861 cgcgcggcgg gcgtgcgggt gatcggcctc gggcgcggct ccaccgccgc cgtatggtcg
     7921 tgggccccgc tcgtgcggct cctgcgccgc gagcgcgtgg acgtgctcca ctcgcacaag
     7981 ttcggctcga acgtgtgggg ggtcgtgctg ggcgggctcg ccggggtgcc ggccgtggtg
     8041 gcgcacgagc acacgtggtc gtacgaggga cagccgctgc ggaggttcct cgaccgcgag
     8101 ctgatcgccc ggcgcagcga cgccttcgtg gcggtctcgc acgaggaccg gcgccggatg
     8161 atcgaggtgg agcggatcga ccccgaggac gtggtgttcg tgccgaacgg catcgacgcg
     8221 ctgccggacg gcgacggcgc ccgcgtgcgc tccgagctgg gaatcgagcc cggggcgccg
     8281 gtgatcggtg ccgtggccgt gctgcgcccg cagaaggcgc tccacctgct cgtgcgcgcc
     8341 gccgcggagc tggtgcgcga gcacccgggg ctgcgggtgc tgatcgcggg cgacgggccc
     8401 gagcgcgagg cgctcgaggc actcgtgcgc gagctcggcc tggagggcgt cgtgacgctg
     8461 ctcgggctgc ggcgcgacgt gccggacctg ctggccgcgc tcgacgtggc cgtggtctcc
     8521 tccgacttcg agggcacccc gctcgcggtg ctcgagtaca tggacgcggg gctgccggtg
     8581 gtcgccacgc gcgtcggcgg cctgtccgac ctgatcgagc acggccgtca cggcctgctg
     8641 gtcgacccgg gtgacgcggg cgtgctggcc caggcgatcg gcacgctgct gcgcgacccc
     8701 gagcgcgccc gcgagctggg cgcgcgcggc cgcgagcggc gccgcgcgga gttctcgatc
     8761 gaccgcacgg tgcgcacgct cgaggacatg tacgtgaatt tggcggggcg atgatcgagg
     8821 aggttcgcga gctcgagccc ctgcgggagg actggcagcg gctggcggag gcggcgggga
     8881 gcccgttctc cacctacgag tgggccgaga cgtggtggcg gcactacggc gcgggtcggg
     8941 agctgcacct gctgcgctgc cgcgacgacg cggggcgcgc gttcgcgatc ctcccgctgt
     9001 tcctcgcccg ctcgcgaccg gtgcggatcc tgcgcttggt cgggcagggg gcggccgacc
     9061 agctcatgcc gatctgcgcg ccccaggacg cggacgccgc ggccgccgcg ctgcacgagg
     9121 cactgcgcga cggcctcgcc ggctggcagg cgctgatcgc cgaccgcctg ccgggcgagg
     9181 agtgggccga gcggctcggc gggcgtgctc tcgaccgcac gccctcgccc gtgctcgagg
     9241 ccggtggcgc gagcttcgac gactggctcg ccacgcgctc gaagaacttc cgcgaccagg
     9301 tgaagcgccg cgagcgcaag ctcgcgaagt cgcacgagct cgcgttccgg ctgaccgacg
     9361 accccgcgcg gctggacgag gacctcggca cgctcatacg gctccaccgg gagcgctggg
     9421 gcgacgcgtc tggctcgttc gagggcgggc gcgaggagtt ccaccgcgac ttcgcgcgcg
     9481 tcgcgctgga ccgcggctgg ctgcggctgt ggacgctcga gctcgatggc cgcccggccg
     9541 cgagctggct cggctaccgc ttcggcgggg acgagtggta ctaccaggcc gggcgcgacc
     9601 cggagctcga gcgcgaggcc gtcggcttcg tgctgatggc gcacacggtg cgcgcggcgc
     9661 tcgacgacgg cgtggcccgc taccggctgc tgctcggcgg cgagggctac aaggaccgct
     9721 tcgccaacgc ggaccacggg ctgcacacga cgatgctcgg ccgcggtgta ctcgggcagg
     9781 tgctcgccgt cggcgcggag acagcggcga cgcggcccgc cctgcgggcg cggctcaagc
     9841 gcttcgcgcg ctgaggcgct cggcctgcac cgtcctggcc acgccgagca gcgcggggcc
     9901 caggccgagc agcagccaga gctgcttgcc gaacatctcc gagatgaaga agtcggcagc
     9961 gaggacgccg gtgatcgcga cgagcagggc gcgcgccagc agctcgaggt cacgccggcc
    10021 tattcgatcg aagatccgcg ccgcgcgcag gatgcacagc agggcgaaca ggatgatcgc
    10081 cgcgaacagc gcgaagccga cgaacccgag ctcggccagc acgtggaggt aggtgttgtg
    10141 ggcggacttc gggttgtcga cgatgaactc gtcgcgcttg agggcgcccg gctggaggag
    10201 gtagtggatc gaggcggtct ggaagttccc gacgccgacg cccgacaccg gcttggcctg
    10261 gaccatgcgc agcccgacct tccacaggtc ggtgcgcccg ctgccgcctt cgacctcggt
    10321 cacccggttg acggcgtcct ggctcgccac gaagccgaag tagaagagac cgccgaacac
    10381 cgcgacgacg gccagcgtga tcgcctggat ccgccagcgg ccggcgacga acgcgctggc
    10441 cgacagcgcc accaccgccg cgatcaggcc gccgcgcgag agcgtgagga acacgccggc
    10501 ggcgcagagg atgctcgcca ccaccgccgc tatgcgcacc atcgggaagc gcttgagcga
    10561 gaacgccagg gcggcgccga ggatcatgcc ggggaccagc accgcggcca gctcgttcgg
    10621 gtcgccgagc gtgccgccca cgcgtgagac gtcgtagtag gccgtgtcct gcggcgggaa
    10681 cagcagcccg taggccgcgg cgacggccgc tccggtcacg tacgcgccga cggtcagcac
    10741 gaactgcttc ggcgtgcgca ccgccgtgta gacgatcagg aacaggatca ggttgagcgc
    10801 gaagcgcatc accgacgtgc gccccgcggc ggcgtcctcg gcccacgtga ggctcaacgc
    10861 cgtccagccg aggaacaggc cgagcacgta ggagaagacc gggtgggctg cgaggaagtc
    10921 gttgcgggcg tcgttgctcg tcgagacctt ggcgagccac gagacggcga tcagcaggcc
    10981 gacgatcttc gagaagctca gcagcgagcc gccgagcgtg ggcagcaggt cgaggaacga
    11041 gacgatcgcg aacaggcaga ggccgaacgt gaggtcgccg agcacgagca ccacgaacgc
    11101 gagcccgatg gcggcggcga ccgccagccg cgggtcgagc ccggccagca ggccgaggcc
    11161 gacggcgatc gtgcccagca gcgcctggta ggcgaggtgc gagccggtgt tcagtcgcgc
    11221 ggccatgccg cggagagggc gctagccgcg tccggcgccc tgcgctgcgc ggcccttgcc
    11281 gcgcgcgggc tcggcgccgg aggccgtgta gtagtagccg tcgccctgcg gcggcacgcc
    11341 gaccacggcg aacccgacgg gctcgatgtc gtggcgggcg agcagctcgc cgaggttcgc
    11401 gagcttcgtg agctgggtgc ggccgaggcg gacgaccacg agcacctcgt ccacctgctg
    11461 cgcgagcggc agggcgtcga tcacctcgct gagcggcggc gagtcgacga tcacgtaatc
    11521 ggcgatccgc ttcgcgtcgg tcacgaggtt ctgcgcggcg ggcaggaaga gctggtcggc
    11581 catgaacccg ctggcgccgc tgctcgtgtc ggcgagcagc acgcgcaggt agcggccgta
    11641 ggcctttgtc tcgaccagcg cgtcctcgag ctcggccatg ccgagcagca cgctgccggt
    11701 gccgtactcg cccttcacac ccagggccgc gccgatcgcg ggacggcgca ggtcggcctc
    11761 gatcaggatc acgctgttgc ccgcgagcgc gagcgacgag gcgaggttga tggcggtcgt
    11821 gctcttgccc tcggacgggg acgggctcgt gacgagcacg gcgggcgcct cgtcgatccg
    11881 catgccgcgg cgcgaggccg cgagcgtggc gcgcagggtc cggtacgact cgagcgtgcc
    11941 cggcgagagc cgttccggag ccagggcgct gtcggtgcgc ttggtctcgc gcgggatgcg
    12001 gccgaggatc ggcaggccgt agagcgtgcg gagctggtcc tcgcggcgca ggcgcgggtc
    12061 gagcacctga agcgcgaagg cgccgccgat gccgaggatc aggccggcga gcacgcccga
    12121 gatcagggag agcttcttct tcggcgacgc ggcctcgtcg gggtcggcgg cgcgggtctg
    12181 gatagagagg gtgggatcgt cctggccacg gagcgtctgg aggcgagcga gctgggagcg
    12241 cagcgaggtg tcctcaccgc cgccggcggc ctcgtcgggc cccgacagcg cctctacgcg
    12301 cgcctcgagg ttcgcgatcg acttgtcgag cgcggcgtgc agcttctggg tctggacctg
    12361 gatcgagctc tcggcgaagc cgttggcgat ctgctgggcc tccttgccgc tcggggcctc
    12421 cgcggtgatc gcgacgatgt tgctctgtgc gaccggctcg gccttcacgt gctcgagcag
    12481 cggctccggc aggccggggg tcttgcggcg caccgcctgg gccacctggc ggttggcgac
    12541 gaggcgggcg gcggtctcga cgtcgcgcgt cgggtcgctc gagccgcgga tgatcggcag
    12601 gccggagaga gccggatcgt cggccgggac cggcgtgacg agcagctcgg actgcgactc
    12661 gtagaccttc ggcgccagca tcacgtaggc gatcgcggcg agcgtcgtca ggaccgtcac
    12721 gatggcgatc agcttgatcc gctcgcggat cgtgttgacg tagcgctgta ggccctgctg
    12781 ctctacgcgc ggacgcagcc attcggcgga ccggctgctc tcggggggtg tcatcgccac
    12841 aactatggca tcacatcggc aataaccatg aggtctttta cggtgttcgg tcccggtccc
    12901 ctcctatggt gcgggccgtg ccccacgtcg acgccagcgt cctggtcccg gtcctcaacg
    12961 aggagcgctt catacgcgac accgtgcgcg cgatgcaggg gcagcgcttc gacggcgagg
    13021 tcgagttcct gttcatggac ggccgctccg atgacgcgac gcgcgcgatc ctcgacgaga
    13081 tcgcgcagga ggacccgcgc gtgcgcgtgc tcgacaaccc gc
//
LOCUS       DASVPL010000059         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_46389, whole genome shotgun sequence.
ACCESSION   DASVPL010000059 DASVPL010000000
VERSION     DASVPL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46389"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VF517_03010"
     CDS             <1..813
                     /locus_tag="VF517_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8051933.1"
                     /translation="VVDDGSLSPVAATGARVVRRAVAGGPSAARNDGWPLARAPLVAF
                     VDDDCVTTPGWLAALVAAAEAAPGAVVQGRTLPDPAEADRLGPFARSLWVEEPGPYYQ
                     ACNILYPRAVLESVGGFDAEAFPHVGEDTDLAWRARAAGARIEWAPDALVHHAVVDLG
                     PAGKLRMAARWTPSIRLFARHRALRREHLTRGLFWKGTHYLLVRSLLGLALRRRSRWL
                     ALWLGAPYVRNLLLRGVSEGGGPAAAPFYLVHDLVELVAVLRGAVRYRTPVL"
     gene            complement(788..2023)
                     /locus_tag="VF517_03015"
     CDS             complement(788..2023)
                     /locus_tag="VF517_03015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8051934.1"
                     /translation="MSAFTKRLAIILAGALALRLLYTIAVAGNEYSPGPFGDFFFFHE
                     IANLIADGHGFAAPFTLEHQGLVEPTAEHPPLWPLVLAAASELGGNGLLAHRLVGVPI
                     GVATVGILGLIGRRLGGERAGLTAAAIGAAHPLLIAADGSLMSETLYGLWVALALLLT
                     LQLRKDPTALRAAALGATIGLAALTRGEGLLLLLLLAAPALWRRKRLLLPALAATVLV
                     LAPWTIRNATTFDRPVLISTNDATVLTGANCHDTYHGRDLGFWRTECRAPVTEEDEAE
                     QSAKWRADGLEYAGDHAGRLVAVVVPVRVARTWDLWQPARQVELAEGRDRTMEALGVI
                     VYFLLLPLAILGAVLLRRREAPLWVLMAPPIAVTFVSVTGYGYTRFRHSADLAIVVLA
                     GLALAHLAERRATARASGT"
     gene            complement(2020..>2409)
                     /locus_tag="VF517_03020"
     CDS             complement(2020..>2409)
                     /locus_tag="VF517_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051935.1"
                     /translation="LGRTRALIVSEFPTAASAALAHALGEPLAIEALRRPDRPDRAAA
                     RGLRARYAEDDGILRRAGALAAHAKSKASPIRTLLTSPAERRALWEVAAVARRVRRAG
                     VTEVHADAGARERAALIAALAGAELRA"
BASE COUNT          293 a          862 c          954 g          300 t
ORIGIN      
        1 gtggtggacg acggctcgct cagcccggtc gcggcgaccg gcgctcgggt ggtccgccgc
       61 gccgtcgcgg gtggtccgtc ggccgcccgc aacgatggct ggccgctcgc gcgcgcgccg
      121 ctcgtcgcct tcgtggacga cgactgcgtg accacgccag gctggctcgc cgcgctggtg
      181 gctgcggcgg aggccgcgcc aggcgcggtg gtgcagggcc gcacgctgcc cgacccggcg
      241 gaggcggacc gcctcggacc gttcgcacgc tccctgtggg tggaggagcc gggcccgtac
      301 taccaggcgt gcaacatcct ctacccgcga gcggtgctcg agagcgtcgg cgggttcgac
      361 gccgaggcgt ttccccacgt cggcgaggac accgacctcg cctggcgtgc gcgggcggcg
      421 ggggcgcgga tcgagtgggc gcccgatgcg ctcgtgcacc acgcggtggt ggacctcggc
      481 ccggccggca agctgcgcat ggccgcgcgc tggacgccgt cgatccgcct gttcgcgcgc
      541 caccgcgcgc tgcggcgcga gcacctcacc cgcgggctct tctggaaggg cacgcactac
      601 ctgctggtgc gctctctgct cgggctcgcg ctgcggcggc gctcgcgctg gctcgctctc
      661 tggctcggcg cgccctacgt gcgcaacctc ctgctgcgcg gcgtgagcga gggtggcggg
      721 cccgcggcgg ctccgttcta cctcgtccac gacctcgtgg agctggtcgc cgtgctgcgc
      781 ggcgcggtca ggtaccggac gcccgtgctg tagcgcgccg ctcggccagg tgcgcgagcg
      841 cgaggccggc gagcaccacg atcgcgaggt cggcggagtg gcgaaagcgg gtgtagccgt
      901 acccggtgac gctcacgaac gtcacggcga tcgggggcgc catcagcacc cagagtggcg
      961 cctcgcggcg gcgcagcagc acggccccga ggatcgccag cgggagcagc aggaagtaga
     1021 cgatcacgcc aagcgcctcc atcgtgcggt cgcgcccctc cgccagctcg acctggcggg
     1081 cgggctgcca gaggtcccac gtgcgcgcga cccgcaccgg caccaccacc gcgacgagcc
     1141 gcccggcgtg gtcgccggcg tactcgagcc cgtccgcgcg ccacttcgcc gactgctcgg
     1201 cctcgtcctc ctcggtcacg ggcgcgcggc actcggtgcg ccagaagccg aggtcgcggc
     1261 cgtggtaggt gtcgtggcag ttggcgccgg tgaggacggt ggcgtcgttg gtggagatga
     1321 ggaccgggcg gtcgaaggtc gtggcgttgc ggatggtcca gggggcgagc acgagcacgg
     1381 ttgccgcgag ggccgggagc agcagccgct tgcgccgcca gagcgccggc gctgccagca
     1441 gcagtaggag gaggaggccc tcaccgcgtg tcagcgccgc caggccgatc gttgctccga
     1501 gcgccgctgc ccggagcgcg gtggggtcct tgcgcagctg gagtgtgagc aggagggcga
     1561 gcgccaccca cagcccgtag agcgtttcgc tcatcagcga tccgtcggcg gcgatcagga
     1621 gcgggtgcgc ggcgccgatc gcggcggcgg tgagcccggc ccgctcgccg ccgagccggc
     1681 ggccgatcag cccgaggatc ccgacggtcg cgacgccgat cggcacgccc accagccggt
     1741 gcgcgagcag cccgttccca cccagctcgg aggcggcggc gagcacgagc ggccacagcg
     1801 gcgggtgctc ggccgtgggc tcgaccagcc cctggtgctc cagggtgaac ggcgcggcga
     1861 acccgtgccc gtccgcgatt aggttcgcga tctcgtggaa gaagaagaag tcgccgaacg
     1921 ggccggggct gtactcgttg cccgcgacgg cgatcgtgta gaggagccgc agggcgagcg
     1981 ccccggcgag gatgatggcg aggcgtttgg tgaaggcgct catgcgcgca gctcggcccc
     2041 ggcgagcgcg gcgatcaggg ccgcgcgctc acgggccccg gcgtccgcgt gaacctcggt
     2101 gacgcccgcc cggcgaacgc gccgcgcgac cgccgcgacc tcccacagtg cccgccgttc
     2161 ggcgggtgaa gtcaaaagcg tccttattgg ggacgctttt gacttcgcgt gagcagcgag
     2221 cgccccggcg cggcgcagga tcccgtcgtc ctcggcgtag cgggcgcgca gcccgcgcgc
     2281 cgcggcgcgg tccgggcgat ccgggcggcg gagggcctcg atggcgagcg gctcgcccag
     2341 cgcgtgggcg agcgcggcgg aggcggcggt cgggaactcg gagacgatca gcgcgcgggt
     2401 gcgcccgag
//
LOCUS       DASVPL010000060         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_124122, whole genome shotgun sequence.
ACCESSION   DASVPL010000060 DASVPL010000000
VERSION     DASVPL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124122"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VF517_03025"
     CDS             <1..488
                     /locus_tag="VF517_03025"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8051936.1"
                     /translation="GRKIRTLPARFGHTTACEITDEGDVRELGRHCMDALGLHGVAKL
                     DFKRSPAGELKLLEVNPRFNLWHLPGAVAGVNLPALVYADLAGLPRPRAELTRRGVRW
                     SLPWNDLRAARQQGVGLARWLRWQAGCETRHIVALDDPMPFVRGFAWRRIRRRLGPAR
                     A"
     gene            628..>1912
                     /locus_tag="VF517_03030"
     CDS             628..>1912
                     /locus_tag="VF517_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX8051937.1"
                     /translation="MANGLVRELRDVAPRDGAAGLPVRDARAAERLVRGPLLRFEVMR
                     RLLRVALLFAIDVGAILLAIWTALELKLLVRGHSDLALAWGQTKTHAPLACLVTVLLF
                     ARSGLYGDRAVRPGFARVVASLFQVTLLILVFTIVQGQEFSSYYIFYGSLLFALVYVS
                     GLRHAFDRASGALLRASGYRRRAVLVGSGPQIEAVAHALAAGGNSELEPIGFVSLTPL
                     PENGLKDLGPLDELEQRFETTDIDEVLIADPAFPEELAVDLVDRCHRHGVRVRVAPST
                     MEILMDRVEFVPGQTVPLFELKPPVFEGVDFVVKRTFDMVVAGLVVVVLAPVMLVVAL
                     AIKLTSRGPVIYRSSRPGIGGMPFACFKFRTMYGDADARQADLEPSNEKGGAIFKIRN
                     DPRVTPIGRFLRRWSIDEMPQLFNVLRGQMSLVGPR"
BASE COUNT          229 a          708 c          673 g          302 t
ORIGIN      
        1 cgggccgcaa gatccgcacg ctgccggccc gcttcggcca caccaccgcg tgcgagataa
       61 ccgacgaggg cgacgtgcgc gagctggggc gtcactgcat ggacgcgctc ggcctgcacg
      121 gcgtcgcgaa gctcgacttc aagcgctcgc cggcgggcga gctcaagctg ctcgaggtga
      181 acccgcgctt caacctgtgg cacctgcccg gcgccgtcgc cggcgtgaac cttcccgcgc
      241 tggtctacgc cgaccttgcg gggctgccgc ggccgcgcgc cgagctgacg cggcgcgggg
      301 tgcgctggtc gctcccctgg aacgacctcc gggcggcccg ccagcagggc gtcggcctcg
      361 cgcgctggct gcgctggcag gccggctgcg agacgcgcca catcgtcgcg ctggacgacc
      421 cgatgccgtt cgtgcgcggc ttcgcgtggc gccgcatccg gcgccggctg ggccccgcgc
      481 gggcctgacc gacgaccagc ggcgtcgcta tgctcgcgcc cttggggagt cgactgcggt
      541 gaatcccgct cacagcgccg gatcgcggcg tcgacaccgc taccttgtcc ccgcttcgct
      601 gttcaagccc gtgcggtacc caccaccatg gccaacggac tcgtaaggga gctcagggac
      661 gtcgcgcccc gcgacggcgc ggccgggctc ccggtccgcg acgcgagggc cgccgagcgg
      721 ctcgtgcgcg gtccgctcct gcgcttcgag gtgatgcgcc ggctgctgcg cgtggcgctc
      781 ctgttcgcga tcgacgtggg cgcgatcctg ctggcgatct ggaccgcgct cgagctgaag
      841 ctgctggtgc gcggccactc cgacctggcg ctcgcctggg gccagaccaa gacccacgcg
      901 ccgctcgcct gcctcgtcac cgtgctgctg ttcgcgcgct ccggcctcta cggcgaccgc
      961 gccgtacgcc cggggttcgc gcgcgtggtc gcgtcgctct tccaggtgac gctgctgatc
     1021 ctggtgttca cgatcgtcca gggccaggag ttctcctcgt actacatctt ctacggcagc
     1081 ctgctcttcg cgctcgttta cgtgtccggg ctgcgccacg ccttcgaccg agccagcggc
     1141 gcgctcctgc gcgcgtcggg ctaccgccgc cgggccgtgc tcgtgggcag cggcccgcag
     1201 atcgaggccg tggcccacgc gctcgccgcg ggcgggaaca gcgagctcga gccgatcggg
     1261 ttcgtctcgc tcacgccgct gccggagaac gggctgaagg acctcggacc gctcgacgag
     1321 ctcgagcagc gcttcgagac gacggacatc gacgaggtgc tgatcgccga ccccgcgttt
     1381 ccggaggagc tggccgtgga cctcgtggac cgctgccacc gccacggcgt gcgcgtccgc
     1441 gtggcgccgt ccacgatgga gatcctgatg gaccgggtcg agttcgtgcc cggccagacg
     1501 gtgcccctct tcgagctgaa gccgccggtg ttcgagggcg tcgacttcgt cgtcaagcgc
     1561 accttcgaca tggtcgtggc ggggctcgtg gtcgtcgtgc tggcgcccgt catgctcgtg
     1621 gtcgcgctcg cgatcaagct cacctcgcgc ggcccggtca tctaccggtc ctcgcgcccc
     1681 ggcatcggcg gcatgccgtt cgcctgcttc aagttcagga cgatgtacgg ggacgcggac
     1741 gcgcgccagg ccgatctcga gccgagcaat gagaagggcg gcgcgatctt caagatccgc
     1801 aacgacccgc gcgtcacgcc gatcgggcgc ttcctgcggc gctggtcgat agacgagatg
     1861 ccgcagctct tcaacgtgct gcgcggccag atgtcgctcg tcggcccgcg gc
//
LOCUS       DASVPL010000061        16452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_320672, whole genome shotgun sequence.
ACCESSION   DASVPL010000061 DASVPL010000000
VERSION     DASVPL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16452
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320672"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VF517_03035"
     CDS             complement(<1..628)
                     /locus_tag="VF517_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051938.1"
                     /translation="MNGHTAIIKSFGTTGVLLVLALVLLAIVSAIVAFDRWPESGSSS
                     TVERIAVDRPEARRVETQLVAERRPASLVRGVVVVRSSGTSATASGAGDVFLVADGGR
                     GGQSDGGGFGPPPPPVPFDAPGEGGGTGSRLGGNAAASTPLPGGAPPPSENPIQRAAC
                     DARQALGQAGTPLDPACKSGSRELVRQVIADAGAVTGAVPEVAGVELQL"
     gene            complement(678..1250)
                     /locus_tag="VF517_03040"
     CDS             complement(678..1250)
                     /locus_tag="VF517_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051939.1"
                     /translation="MILFLGLALAPRAFEFRAWPEPSRHEAVEEVVDRPVAPVAEVHV
                     AGVTARSSRREPGGSVRVERRRRPERAPARTPKAAPSRAGSRDNAAGRSGRTAPVAPQ
                     APEIVVETPAPPAEVEEPPAQPAQLADRAPVDPVLRPEPEAIVEEGSPLNTFGTRDAG
                     RGAREGDRKARGRGRHGRGNGHRGHHAERR"
     gene            complement(1372..2109)
                     /locus_tag="VF517_03045"
     CDS             complement(1372..2109)
                     /locus_tag="VF517_03045"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX8051940.1"
                     /translation="MQPELQIGPLELKTFGICFALGFVAAGLVGARRLRELDLPVDWA
                     YEIIFAGLAGGFVGARVDYVIQNWDTASEDLVGAAFSGSGLVWLGGVVGGAIAVLAWA
                     RWRGFLGLALLDLTAPLIALGYAIGRVGCQLSGDGDYGKASDLPWAMAYPDGTEPIDT
                     AVHPTPIYESIAMGLVALALWRLRDRLRPGLIFALWLVLTGLERLLVEFVRRNETAAL
                     SLTLAQLVSVAMIAAGAAWLVRARQPA"
     gene            complement(2134..3312)
                     /locus_tag="VF517_03050"
     CDS             complement(2134..3312)
                     /locus_tag="VF517_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8051941.1"
                     /translation="MRRRFFTYITLALGMYFLLPLPAQTKPLPQKIDEARERVERKRG
                     EERVLSTDISRYAVRIRGLQGEIRSVSRRQAKLEVEVDQKRAELEGIRDRLDSARIRL
                     GLLRERLIESQKTLAARLVAIYKDGDPDILSVVLDSTSFGDFLDRTEFLDRIADQDQK
                     VITRVRRVKREVADLTKQLTGLETQAEAARNTILSKRNEVLVVKNRIVSRRDDLDRAR
                     DGRAAILARVRSSRGHAEDDLASLERIQARVSAQVRSAGAGAATPAAGPIRRGSGRFI
                     WPVNGSVVSPFGQRWGRLHAGIDIGSPTGTPIRAAASGRVILMGPTGGYGNYTCVGHG
                     GGISTCYAHQSRYGTSNGASVRQGQVIGYVGCTGHCFGPHLHFEVRVGGSPVDPLGYL
                     "
     gene            3573..5333
                     /locus_tag="VF517_03055"
     CDS             3573..5333
                     /locus_tag="VF517_03055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8051942.1"
                     /translation="MSTAADLSRSTDPAAAGNLDAGSRALLDLSLRQGVSDEDIARVL
                     GTEAGDVARRREEALAQVAQSLAARGGFAPGASDLVSDASGFAPGASGLAPDASSFAP
                     GAGDLAPGTATPDPVGPRASAPGLFPGAIGPRVEAADPGERAAWTSRGSRREEPKSSA
                     VDVVLRVACALYLVVLLAGVVVFKAAFVQAITTDPLLTAYGLLVCGYIVSRFVLSLLY
                     RGAPDRGLEPRVAIVMPAFNEEEAIARSLRSLLAVDYPHAKLEIVAVDDGSTDGTSAA
                     MRAVAAQTDRVRVIEFPENRGKRAAMAAGIAATDAEIVAFVDSDSTIERDALRKLVQG
                     FADPEVGAICGHADVANPRASLMARMQAVRYFVAFRVVKAAESVFSTVTCCSGCFSAY
                     RREAILPRLDWWANQSFLGVPSTFGDDRSLTNCVLRDWKVRYEAKAVSHTLVPTTFST
                     FMRQQLRWKRSWTRESLIVSRFMWRKNPLAALATYIGILLPLVAPVIAVRAVLWMPLV
                     EGEGAPLIYMLGVYAMAIAYGLYYAAKHPRYDTLWVFGVAFCFFYLAFLLWQTYWAIL
                     SVRTASWGTRPATAGTEGPT"
     gene            5330..6448
                     /locus_tag="VF517_03060"
     CDS             5330..6448
                     /locus_tag="VF517_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8051943.1"
                     /translation="MSRLELLLERPLALLLLPLSILPFLLLVPQMVESRELYERRHES
                     GPLPAPAVVLTAAQVHRFQPVAPYRGAVPVLAYGRVADDASDARAVSRRTFAEQMAAL
                     KRMGFQTISTEQFARFRAGDGTGLPARPILITFDGGRLDSYRAADRVLAQHGLRATMF
                     VSTAPIEAEDPSHLTWAELHRMADSGRWDVEPQTHDGARRVAYDELGSTAPFYAVRRY
                     TRSGGLETFADYERRVTGDLFAVAERMRDQGFEPHALALPGGEYGQGRTNDPAIAPFM
                     RALLARQFGVYFARHQRNEPEYARALGEPQRLELHAGTTTDRLYMWLRDHSPGAAKPR
                     GGAPEAEPRGSSARRRGSSAKRRGPSAKPRGSDRRARD"
     gene            6448..8280
                     /locus_tag="VF517_03065"
     CDS             6448..8280
                     /locus_tag="VF517_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX8051944.1"
                     /translation="MRRLVLVLAVLAATFAAATAPASAARISCDRNAGLGFERAPGRT
                     AGTLTWSRRPSAAKGTRYRVFRNGAVVGQTRGLKMRVRVRIGGTYTFAVRPVSKRGKV
                     ARCGQRLRRKVAYVRPGRATAIGATNPAGAASVRLSWEPATRGDGRLVGYRVFRDGAT
                     VGQTAETGIDVRISSNRRYELRVAAVDSRGTLGALSDAVTVETGHEPPPAPTGLSASD
                     VDAGAVTLSWAASAPPRGRVVGYRVFRDDRLVRQVQGTSFRLTNLAAQTTHGLTVVAV
                     DSLGYLSPPSAPLAVTTARPEQTAGRAHAFLLASTDRSFEDLQANYRRIGTVYPTYYD
                     CSATAAVTGRDDPLVTNWSLQRGLRVLPRFNCQRNTVLNRVLNEPALREAWIARILEL
                     VETHGYSGANLDFETGYGTDRDAYTSFVAELAQRLHDRGKLLSIAVSAKRADVANHPR
                     STFFDYIALSQHADTIFVMAWGFHWTTSGPGAPDDIEWVKGVVNYVKTMPNRHRFVLG
                     SPIYGMDWAGDGGSDQPADHYEHEDVMALAARVGATPTRDAATDTMHFDYVDERGTPH
                     QVWYADATTLATRIALAREAGIGTGFWRLGREDQRLWSDPLLVP"
     gene            8471..9388
                     /gene="htpX"
                     /locus_tag="VF517_03070"
     CDS             8471..9388
                     /gene="htpX"
                     /locus_tag="VF517_03070"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX8051945.1"
                     /translation="MARTRTSFGRDTGLQVRMILTLFLLGLLYAVFAGVIVAAGAGVT
                     IMVVVLGALMAAQFFFSDKLALAAVGAKEVGPDQAPGLHAMIDRLCIQADLPKPKIAV
                     ADSPVPNAFAMGRSPKSATVCATTGIMETLQPHELEGVMAHELSHVKNRDVLIITIAS
                     FFASIASMIVQFGFFFGGGHNDYGDDDNPSILVVILVSMVVYVISFFLMLALSRYREF
                     SADRTAALITGRPSALASALMRISGKMQQIPESDLREVGRMNAFFIVPTSVKKTAATL
                     FSTHPPMEARIEALNKLEAQLQAGGAIAR"
     gene            9410..9979
                     /locus_tag="VF517_03075"
     CDS             9410..9979
                     /locus_tag="VF517_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051946.1"
                     /translation="MGFLDSLFGGSKKLKAPAQDRLFAMSTAHITFETSLGMTHRTAS
                     GIVFQPLATADFAGIVKEAEELLRGQAEDTGTTVETSDDQFGYRWMVLRDDDFEDLVV
                     AVNVVSSQLEASGYGDRLLAAVFAFEQDGKPVYFIYNFKRGAYYPFVPKGERQRDNEH
                     ELRLKAQVGTELPVEQELDRWFPLWEIPL"
     gene            complement(10235..10513)
                     /locus_tag="VF517_03080"
     CDS             complement(10235..10513)
                     /locus_tag="VF517_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051947.1"
                     /translation="MIVRILSEGQFKLADDVQTKLNEIDNEAVAAVEAGDEARFHELF
                     EQMIALVESGGEPLGDDELVESQIIIPPRNLTFAEAKGEFTGDGIIPD"
     gene            complement(10510..11313)
                     /locus_tag="VF517_03085"
     CDS             complement(10510..11313)
                     /locus_tag="VF517_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX8051948.1"
                     /translation="MPGLAGRMSTVVKSKISKLLDRAEDPGETLDYGYQKQVELLQNV
                     KKGIADVVTSKKRLQMQSKKLEESVVKLDTQARQALSAGREDLARTALERKTLAQSEL
                     QSLDTQIKELEGQQEQLIANEKKLRSKIEAFRTKKEVIKAQYSASEAQVRISEAANGV
                     GEEMADLGLAMQRAQDKTEDMRARASAVEELEKAGAFEDITALGSGETDIDRELSQLT
                     SGVQVDDELAKMRAELGTGSGGGEEAPALPASGTGEQGGGTTPSQGGAA"
     gene            complement(11411..13837)
                     /locus_tag="VF517_03090"
     CDS             complement(11411..13837)
                     /locus_tag="VF517_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8051949.1"
                     /translation="MSRLTLALVVAALCALAAAPAALAADYAGTALNILPSGQYQTPG
                     PEADKQAQMYNALTPRYDNVTDADLPSFFKSEALGPTTVVREETIPGRPGVLIKRDEY
                     NVPHIYGQTNDDVIFGAGWAIASDRSLLLNQGRFNGITAAVDAPNLSAIDLIRELYQF
                     EPSAQANAIVARQTKVIQQAGPKGVQLLHDIDLYVGGINAWYAANSPSSPKFTRNDIY
                     AFNAIKGQFLGEGGGSEPATSQLLDGLQRKFGAKRGFAIWQDLRNRKDPEQKATLRNK
                     APYDTATGSRKGTVTLRNGSFKGEGGLSGASSARAEASNVLMVSGQRSATGHPLFVGG
                     PQISYFFPGLVAEMGLYGPDIQVRGATSVPFPGYMLIGRGEDFAWTLTSAGGDIIDTY
                     AETLCGGSKTKYLYKGRCRSMDLVNAGTLTKGTTKRVVRFRQTVHGPVVGYAKTSGGK
                     TVAISRRRSSYGKDTLDQLFFQDLTFGRVKSYDDFAASAAQTPQTFNAFYADHQNTGL
                     YTTGLLPLRPKGADGSLVNDGRGGYEWKGYLRASQHPRGSNPDNGLIVNWNNRPARGF
                     VSGDDRFGSEGPVPRNDLLQRELARTDKHSLGSVTAAMNAAAVQDPRGNLFWPTMRAM
                     LSKVDSPSPRAAAMVAQLDAWNARRAPRIDQDRDGNMDDAGVPIMDGIWRQVADASLC
                     DAIGKPLCEQLADTLHGRFATPPGGQYSGWHHYMDKDFRSLLGAKVRGAFSQHYCAKS
                     AKACATKLWKAIDAAGTSLAEKFGPDPATWRQPRAQTQIKFSPLPLIDMDYTNRPSGI
                     QQVISFNGHR"
     gene            complement(13853..14647)
                     /locus_tag="VF517_03095"
     CDS             complement(13853..14647)
                     /locus_tag="VF517_03095"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX8051950.1"
                     /translation="MISLDPAGLVVVALLVWLHARATRRLARRGHRVPRGQVAAWYGG
                     VGLIALGLFGPPDRLGEDLLVAHMGQHLLIADLAGPLLLVGARSPVLFFMPPRSLLVA
                     LARQPRLRALSRSVRQPLVALGVYITVLYTWHLPFAFEAALGNDVVHGLQHQSFVAIG
                     LLVWWPVLEPNRSRLPGELWKAGFVLGTRVLTMFVGMAFIVQRTPAYDGYSGAHGISA
                     LADQQLAGGLMMVVDLLIMLSALAFFFWRAGVDHDRAEARDVARVG"
     gene            complement(14703..16055)
                     /locus_tag="VF517_03100"
     CDS             complement(14703..16055)
                     /locus_tag="VF517_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8051951.1"
                     /translation="MSDTIDWSVNYDGGTYAVDFRLTDEQKEWQAHCHRFAEEHMRPV
                     ADKHDREQSVPWEVMKEARKWDLSGLDYIQKMANDPDGLFGVIYAEELHWGCAGIALA
                     ISASSLAAAGVAASGTPEQIGRWIPEIYGTSKEDMKLGAYAVTEAGAGSDVKSLRTTA
                     KRDGDEWVLNGTKVFISNGGIADVTVVVATVDATLGHRGHASFIVPKDTPGFSMGKKE
                     DKIGIRASQTAEVVLDNCRLPMEYLLGGIEKLEAKLERARSGQATGKQSGALATFEIT
                     RPLVGAAALGIARAAYEWTLDYLEEKSDADGPLLDQQRHQQVLADVATEIDAARLLVY
                     RAAWMGRNGVPMTGGQGSMSKLKAGDVTMWATTTLMDLVGEYAQTTECPLEKWFRDAK
                     IYQIFEGTAQMQRLVVARMQRQDYQERINTAQEILEQAPGASTGGVIGDAIDAVTSAA
                     "
     gene            16101..>16452
                     /locus_tag="VF517_03105"
     CDS             16101..>16452
                     /locus_tag="VF517_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051952.1"
                     /translation="MPRLTLLVAAALFALTALGGCSLKSEDSGGDPAQRVGEGVSSGD
                     DSAVEALGFPTTATRDTIRVPGSDPAADAAGVASALFPATTTETRPPAVALVDGDPWQ
                     AGVAAAVRAGAPLRA"
BASE COUNT         2212 a         6009 c         5816 g         2415 t
ORIGIN      
        1 cgagctggag ctcgacgccg gcgacctcgg ggacggcgcc ggtgacggca ccggcgtccg
       61 cgatcacctg acggacgagc tcgcggctgc cggacttgca ggccgggtcg agcggggtac
      121 cggcctgacc gagggcctgg cgcgcatcgc aggcggcgcg ctggatcggg ttctcgctgg
      181 gcggcggagc gccgccgggc agcggagtgc tcgcggccgc gttgccaccg aggcgcgagc
      241 cggttccccc gccctcgccc ggagcgtcga acggcaccgg cggcggcggc ggaccgaagc
      301 cgccgccgtc ggactggccg ccgcgccccc catcggcgac caagaacaca tcgcctgctc
      361 ccgaggccgt cgcggacgtg ccggacgagc gaacgaccac gaccccccgc accaaggagg
      421 ccgggcgacg ttcggcgacc agctgcgtct cgacgcgccg ggcctcgggt cggtccaccg
      481 cgatgcgctc caccgtgctc gagctcccgc tctccggcca gcgatcgaac gcgactatcg
      541 cgctcacgat cgccagcagg accagtgcca ggaccaggag gactccggtc gtaccgaagg
      601 acttgatgat tgccgtgtga ccgttcacgg ggacgcgcag gggtagtacg cggtctattc
      661 cccgtaaggg ctgaggccta ccgtctttct gcgtggtggc cgcggtggcc gttgccgcgc
      721 ccgtgccggc cgcgtccgcg tgctttccgg tcgccctcgc gggcgccccg gccggcgtcg
      781 cgggtgccga aggtgttcag cggagagccc tcctcgacga tcgcctccgg ctcgggccgc
      841 aggaccgggt ccaccggcgc gcgatcggcg agctgggccg gctgcgccgg cggctcctcc
      901 acctcggcgg gcggggcggg ggtctccacg acgatctcgg gcgcctgagg cgccacgggg
      961 gcggtcctgc cggagcgtcc ggcggcgtta tcacgcgagc ccgcgcggct cggagcggcc
     1021 ttcggggtac gcgcgggggc gcgctcgggg cgccgcctac gctcgacgcg cacggacccg
     1081 ccgggctcgc ggcgcgagct ccgcgcggtc acgcccgcga cgtgcacctc ggcgaccggc
     1141 gcgaccgggc gatcgacgac ctcctcgacc gcctcgtggc gcgagggctc gggccaggca
     1201 cggaactcga atgcccgcgg cgcgagggcc agaccgagga agaggatcac cgctaatagc
     1261 cccgcgacgg ggaggctggg gatgcctgac ggattggagg gcgtaggcat ggtacggctc
     1321 ctatcggcac ccccgcaaaa acctttaggc ctgaacgcag ttcgtggacg atcaggcggg
     1381 ttggcgcgca cgcacaagcc acgcggcgcc ggcggcgatc atcgccacgc tcaccagctg
     1441 cgccagcgtc aggctcaggg cagccgtctc gttgcggcgc acgaactcga cgagcagccg
     1501 ctcgagcccc gtcagcacga gccagagcgc gaagatgagc cccggccgca gccggtcgcg
     1561 cagacgccag agcgcaaggg cgaccaaccc catcgcgatc gactcgtaga tcggcgtcgg
     1621 gtggaccgcg gtgtcgatcg gctcggtccc gtcgggatag gccatcgccc acggcaggtc
     1681 ggacgccttg ccgtagtcgc cgtcgccgga gagctggcac ccgacgcgcc cgatcgcgta
     1741 cccgagcgcg atcagcggag cggtgaggtc gagcaacgct aggccgagga agccgcgcca
     1801 gcgggcccag gcaagcaccg cgatcgcgcc gcccaccacc ccgccgagcc acacgagccc
     1861 ggagcccgag aacgccgcgc cgaccagatc ctcggaggcc gtgtcccagt tctggatgac
     1921 gtagtcgacg cgcgcgccga cgaacccgcc ggcgagcccc gcgaagatga tctcgtaggc
     1981 ccagtcgacg ggtaggtcga gctcgcgcag ccgccgggcg ccgaccagcc cagcggcgac
     2041 gaagccgagg gcgaagcaga tcccgaaggt cttgagctcg agcgggccga tttgcagctc
     2101 gggctgcacg gccgcgaggc tatccggacg gcgctacagg tacccgagcg ggtccacggg
     2161 gctgccgccc acgcgcacct cgaagtgcag gtgcggcccg aagcagtgcc cggtgcagcc
     2221 gacgtagccg atcacctggc cctgccggac actggcgccg ttcgaggtcc cgtagcgcga
     2281 ctggtgggcg tagcaggtgg atatcccgcc gccgtggccg acgcatgtgt agttgccgta
     2341 gccgccggtg ggccccatca ggatcacgcg gcccgaggcg gcggcacgga tcggcgtgcc
     2401 ggtgggcgag ccgatgtcga tgcccgcgtg caggcggccc cagcgctggc cgaacggcga
     2461 gaccaccgag ccgttgaccg gccagatgaa gcggcccgag ccgcggcgga tcgggccggc
     2521 ggcgggcgtc gcggcgccgg cgccggcgga gcgcacctgc gcgctcacgc gcgcctggat
     2581 ccgctcgagc gaggccaggt cgtcctccgc gtgtccgcgg ctgctccgga cgcgggcgag
     2641 gatcgccgcg cggccgtcgc gggcgcgatc gaggtcgtca cggcgcgaga cgatgcggtt
     2701 cttcaccacg agcacctcgt tgcgcttcga gaggatcgtg ttgcgagcgg cctccgcctg
     2761 cgtctcgagg ccggtgagct gcttggtgag gtcggccacc tcgcgcttca cgcgccgcac
     2821 gcgcgtgatg accttctggt cctggtcggc gatgcggtcg aggaactcgg tgcggtcgag
     2881 gaagtcgccg aagctggtcg agtcgagcac cacgctgagg atgtccgggt cgccgtcctt
     2941 gtagatcgcg acgaggcggg cggccagggt cttctgcgac tcgatcaggc gctcccgcag
     3001 caggccgagg cggattcggg cgctgtcgag ccggtcgcgg atgccctcca gctcggcgcg
     3061 cttctggtcg acctccacct ccagcttggc ctggcggcgc gaaaccgagc ggatctcgcc
     3121 ctggagcccg cggatccgca ccgcgtagcg cgagatgtcg gtcgagagca cccgctcctc
     3181 gccgcgcttg cgctcgacgc gctcgcgggc ctcgtcgatc ttctgcggca gcggcttggt
     3241 ctgtgccggg agcggcagca ggaagtacat cccgagcgcg agcgttatgt acgtgaagaa
     3301 tcggcgtcgc atggctttga gcgcagcgcc gtgaccggcg cgcgtttacc cgcgttatgc
     3361 agggctttcc tgctcaacga ggcgcccggc aggctagcac ggctctaacg ctccagccca
     3421 ccttcaggct gaggttggtc gagactttgc aagcaggagg aggacgcccc tcgacagcga
     3481 ctgggcgcaa tttcccacac aaggttcaag tccggctgcc gataagtggg ctgggcagca
     3541 agcccgccga accggacgta cccctccccc cgatgtcaac cgccgccgac ctctcacgtt
     3601 caaccgatcc cgcagccgcc gggaacctcg atgcgggcag ccgcgcgctg ctcgacctgt
     3661 ccctgcgcca gggcgtctcc gacgaggaca tcgcccgcgt gctcggcacc gaggccggcg
     3721 acgtggcgcg ccggcgcgag gaggcgcttg cgcaggtggc gcaatcgctg gcggcgaggg
     3781 gcggcttcgc gccgggggcg agcgacctcg tgtcggacgc gagcggcttc gcgccgggag
     3841 cgagcggact cgcgccggac gcgagcagct tcgcgccggg ggcgggcgac ctcgcgcccg
     3901 gcaccgcgac cccggacccg gtcggcccgc gtgcctccgc ccccggcctc ttccccggcg
     3961 ccatcggccc ccgcgtggag gcggccgacc ccggcgagcg cgccgcctgg acctcgcgcg
     4021 gctcgcgccg cgaggagccg aagtcctcgg cggtcgacgt cgtgctgcgc gtcgcctgtg
     4081 ccctctatct cgtcgtgctg ctcgccggcg tggtggtctt caaggccgcc ttcgtgcagg
     4141 cgatcaccac cgacccgctg ctcacggcgt acgggctgct cgtctgcggc tacatcgtca
     4201 gccgcttcgt gctgagcctg ctctaccgcg gcgcgcccga ccgcggcctc gagccgcgcg
     4261 tcgcgatcgt gatgcccgcg ttcaacgagg aggaggcgat cgcgcgctcg ctgcggtcgc
     4321 tgctcgcggt cgactacccg cacgcgaagc tcgagatcgt cgcggtggac gacggctcca
     4381 ccgacggcac ctccgcggcc atgcgcgcgg tcgccgccca gaccgaccgc gtccgcgtga
     4441 tcgagttccc ggagaaccgc ggcaagcgcg cggcgatggc cgccggcatc gcggccaccg
     4501 acgccgagat cgtcgccttc gtggactccg acagcacgat cgagcgcgac gcgctgcgca
     4561 agctcgtcca gggcttcgcc gacccagagg tcggcgcgat ctgcggccac gccgacgtgg
     4621 ccaacccgcg cgcgtcgctc atggcgcgga tgcaggccgt gcgctacttc gtggccttcc
     4681 gcgtggtcaa ggcggccgag tcggtgttca gcactgtcac ctgctgctcg ggctgcttct
     4741 ccgcctaccg ccgcgaggcg atcctgcccc ggctcgactg gtgggccaac cagtcgttcc
     4801 tcggagtgcc ctcgacgttc ggcgacgacc gttcgctgac caactgcgtg ctgcgcgact
     4861 ggaaggtgcg ctacgaggcg aaggcggtct cgcacacgct cgtcccgacg accttctcga
     4921 cgttcatgcg ccagcagctg cgctggaagc ggagctggac gcgcgagtcg ctgatcgtct
     4981 cgcgcttcat gtggcgcaag aacccgctgg cggcgctcgc cacctacatc gggatcctgc
     5041 tgccgctcgt cgctcccgtc atcgccgtgc gcgcggtgct ctggatgccg ctcgtcgagg
     5101 gcgagggcgc gccgctgatc tacatgctgg gcgtctacgc gatggcgatc gcgtacgggc
     5161 tctactacgc cgccaagcac ccgcgctacg acacgctctg ggtcttcggc gtcgcgttct
     5221 gcttcttcta cctggccttc ctgctgtggc agacgtactg ggcgatcctg tccgtgcgca
     5281 ccgcctcgtg gggcacgcgg cccgccaccg ccggcacgga ggggccgacg tgagccggct
     5341 cgagctgctc ctggagcggc cgctcgcgct gctcctactg ccgctgtcga tcctcccgtt
     5401 cctgctgctc gtgccgcaga tggtcgagtc gcgcgagctc tacgagcgcc gccacgagag
     5461 cgggccgttg ccggccccgg ccgtggtgct cacggccgcg caggtgcacc gcttccagcc
     5521 ggtggcgccg taccggggtg ccgtgccggt gctggcctac gggcgcgtcg cggacgacgc
     5581 gagcgacgcc cgcgccgtga gtcgccgcac gttcgccgag cagatggccg cgctgaagcg
     5641 gatgggcttc cagacgatct ccaccgagca gttcgcgcgc ttccgcgcgg gcgacgggac
     5701 cggtctcccg gcgcgtccga tcctcatcac gttcgacggc ggcaggctcg actcgtaccg
     5761 cgccgcggac agggtgctcg cccagcacgg cctgcgcgcg acgatgttcg tgagcacggc
     5821 gccgatcgag gccgaggacc cgtcgcacct gacctgggcc gagctgcacc gcatggccga
     5881 ctccgggcgc tgggacgtgg agccgcagac gcatgacggc gcccgccgcg tggcctacga
     5941 cgagctcggc agcaccgcgc cgttctacgc cgtgcgccgc tacacgcgca gcggcggtct
     6001 cgagaccttc gccgactacg agcggcgcgt gaccggcgac ctgttcgccg tcgccgagcg
     6061 catgcgcgac cagggcttcg agccgcacgc gctcgcgctg ccgggcggcg agtacggcca
     6121 gggccggacg aacgaccccg cgatcgcccc gttcatgcgc gcgctcctgg cgcggcagtt
     6181 cggcgtctac ttcgcgcgcc accagcgcaa cgagcccgag tacgcgcgtg cgctgggcga
     6241 gccgcagcgc ctggagctgc acgccggcac gacgaccgac cgcctgtaca tgtggctgcg
     6301 cgaccactcg ccgggtgcgg cgaagccgcg cggtggcgcg ccggaggcgg agccgcgcgg
     6361 ctcgtcggcg aggcgccgcg gctcgtccgc gaagcgccgc ggcccgtcgg cgaagccgcg
     6421 cggctccgac cggagggctc gcgactgatg cgccggctcg tcctcgtcct cgcggtgctc
     6481 gccgcgacct tcgcggcggc cacagcgccc gccagcgccg cgcgcatcag ctgcgaccgc
     6541 aacgccgggc tcggcttcga gcgcgcgccc ggccgcaccg cgggcacgct cacctggtcg
     6601 cgccgcccgt cggcggcgaa gggcacgcgc taccgcgtgt tccgcaacgg cgccgtcgtg
     6661 ggccagacac gcggcctgaa gatgcgcgtg cgcgtgcgca tcggcggcac gtacaccttc
     6721 gcggtccgcc cggtctcgaa gcgcggcaag gtcgcccgct gcgggcagcg cctgcgccgc
     6781 aaggtcgcct acgtgcgccc gggccgcgcc accgccatcg gagcgacgaa tcccgccggc
     6841 gccgcgagcg tccgcctctc gtgggagccg gccacccgcg gggacggccg cctcgtcggc
     6901 taccgcgtct tccgcgacgg ggcgaccgtc ggccagacgg cggagaccgg gatcgacgtg
     6961 cgcatcagct ccaaccgcag gtacgagctg cgcgtggccg ccgtggactc gcgcggcacg
     7021 ctgggcgcgc tcagcgacgc cgtcaccgtc gagaccggac acgagccgcc gcccgcgccc
     7081 accgggctga gcgcgagcga cgtcgacgcg ggggccgtga ccctgtcgtg ggcggccagc
     7141 gccccgccgc ggggtcgcgt cgtcggctac cgcgtgttcc gcgacgaccg cctcgtccgc
     7201 caggtccagg gcacgagctt tcggctcacg aacctcgccg cgcagaccac gcacggcctg
     7261 acggtcgtcg ccgtcgacag cctcggctac ctcagcccgc cgagcgcgcc gctcgccgtc
     7321 accaccgcgc gccccgagca gaccgccggc cgcgcccacg ccttcttgct cgcgtccacg
     7381 gaccgcagct tcgaggacct ccaggccaac taccgccgca tcgggaccgt ctacccgacc
     7441 tactacgact gctcggccac ggccgcggtc accggccgcg acgaccccct cgtcacgaac
     7501 tggtcgctcc agcgcggcct gcgcgtgctg ccgcgcttca actgccagcg caacacggtc
     7561 ctcaaccgcg tgctcaacga gcccgcgctg cgcgaggcct ggatcgcgcg gatcctcgag
     7621 ctggtcgaga cccacggcta cagcggcgcg aacctcgact tcgagaccgg ctacggcacg
     7681 gaccgcgacg cctacacgag cttcgtggcc gagctcgccc agcgcctcca cgaccgcggc
     7741 aagctgctct cgatcgcggt ctcggccaag cgcgccgacg tggccaacca cccgcgctcg
     7801 accttcttcg actacatcgc cctctcccag cacgcggaca cgatcttcgt gatggcgtgg
     7861 ggcttccact ggaccacctc cgggccgggc gcgcccgacg acatcgagtg ggtgaaggga
     7921 gtcgtgaatt acgtgaagac gatgccgaac aggcaccgct tcgtgctcgg cagcccgatc
     7981 tacgggatgg actgggcggg cgacggcggc agcgaccagc ccgccgacca ctacgagcac
     8041 gaggacgtga tggcgctggc cgcccgcgtc ggcgccaccc cgacgcgcga cgccgccacc
     8101 gacacgatgc acttcgacta cgtggacgag cgcggcacgc cgcaccaggt ctggtacgcc
     8161 gacgcgacca cgctcgccac ccgaatcgcg ctcgcgcgcg aggccgggat cggcaccggc
     8221 ttctggcggc tcggccgcga ggaccagcgc ctctggtcgg acccgctgct cgtgccctga
     8281 gcgccacccg gcgtccccgc gcgcggcgcc cggcgccccc tcgggcgccc gcccagcgcc
     8341 ccctcgcgcg cagcccggca ccccttcggc gcccccgtcc ggcgccccct cgggcgcccg
     8401 tccggcgccc gccgcttccg cgcccgcggc cctttaggtc gacctaagtc cccgccctag
     8461 actcaagggc atggctcgca cacgaacttc attcggcagg gacacgggcc ttcaggtccg
     8521 gatgatcctc acgctgttcc ttctcggctt gctctacgcg gtgttcgcgg gcgtgatcgt
     8581 cgcggccggt gccggcgtga cgatcatggt cgtggtgctc ggcgcgctga tggccgcgca
     8641 gttcttcttc tcggacaagc tcgccctcgc cgccgtcggt gcgaaggagg taggccccga
     8701 ccaggcgccg ggcctccacg cgatgatcga ccgcctctgc atccaggccg atctgcccaa
     8761 gccgaagatc gcggtcgccg acagccccgt gccgaacgcc ttcgcgatgg gccgctcgcc
     8821 caagtccgcg accgtctgcg cgaccacggg gatcatggag acgctccagc cccacgagct
     8881 cgagggcgtc atggcgcacg agctctcgca cgtgaagaac cgcgacgtcc tcatcatcac
     8941 gatcgcgagc ttcttcgcct cgatcgcgtc gatgatcgtc cagttcggct tcttcttcgg
     9001 cggcggccac aacgactacg gcgacgacga caacccgagc atcctcgtgg tgatcctcgt
     9061 gtcgatggtc gtctacgtga tctcgttctt cctgatgctc gcgctctcgc gctaccgcga
     9121 gttctcggcc gaccgcaccg cggcgctgat caccggccgc ccgagcgcgc tcgcctccgc
     9181 gctgatgcgg atctccggca agatgcagca gatccccgag tccgacctgc gcgaggtcgg
     9241 ccgcatgaac gccttcttca tcgtgcccac cagcgtgaag aagaccgccg ccacgctgtt
     9301 ctcgacgcac ccgccgatgg aggcccgcat cgaggccctc aacaagctcg aggcgcagct
     9361 ccaggccggc ggcgcgatcg cccgctaggg ccgcgctagc ctcccgaccg tgggcttcct
     9421 cgactccctc ttcggaggca gcaagaagct gaaggcgccg gcgcaggacc ggctcttcgc
     9481 gatgtcgacg gcgcacatca cgttcgagac ctcgctcggg atgacccacc gcacggcctc
     9541 cggcatcgtc ttccagccgc tcgcgacggc cgacttcgcc gggatcgtca aggaggccga
     9601 ggagctgctg cgcgggcagg ccgaggacac aggcaccacg gtcgagacga gcgacgacca
     9661 gttcggctac cgctggatgg tcctgcgcga cgacgacttc gaggacctgg tggtggccgt
     9721 gaacgtggtc tcgagccagc tcgaggcgag cggctacggc gaccgcctgc tggccgccgt
     9781 cttcgccttc gagcaggacg gcaagccggt ctacttcatc tacaacttca agcgcggcgc
     9841 gtattacccg ttcgtcccga agggcgagcg ccagcgcgac aacgagcacg agctgcgcct
     9901 gaaggcccag gtcgggaccg agctgcccgt cgagcaggag ctcgaccgct ggttcccgct
     9961 ttgggagatc ccgctctagg cgtcacggag cccgtgcgcg cctccgggta gggggcgccg
    10021 cggcgccggt ccacgagggg cggtgtgaac cggcgccgag cggcgcccga cgaccggcgc
    10081 ggggggccga accggtcgct tacgtgggtc caagacgggc acgccgcgaa gcttccatcc
    10141 aggggctccc cgtcttgtgc ctgagtatct cgtccctata acggtggtat cgggacacgc
    10201 gacgcctaaa cgcctatttc gcgaaacggg cccgtcagtc gggaatgatc ccgtcccccg
    10261 tgaactcgcc cttcgcctcc gcgaaggtca ggttccgggg cgggatgatg atctgcgact
    10321 cgaccagctc gtcgtccccg agcggctcgc cgccgctctc cacgagcgcg atcatctgct
    10381 cgaacagctc gtggaagcgg gcctcgtcgc cggcctccac ggccgccacg gcctcgttgt
    10441 cgatctcgtt gagcttcgtc tgaacgtcgt cggccagctt gaactggcct tcggagagga
    10501 ttcggacgat cacgcggcac ctccctgcga gggcgtggta ccgccgccct gctcgcccgt
    10561 tccgctcgcg ggaagcgccg gggcctcctc gccgccacct gagccggtgc cgagctcggc
    10621 gcgcatcttc gccagctcgt cgtcgacctg aacgcccgag gtgagctgcg agagctcacg
    10681 gtcgatgtcg gtctcacccg agccgagcgc cgtgatgtcc tcgaaggcac ccgccttctc
    10741 gagctcctcg acagccgacg cgcgcgcccg catgtcctcg gtcttgtcct gggcgcgctg
    10801 catcgcgagc ccgaggtcgg ccatctcctc gccgacgccg ttggcggcct cggagatccg
    10861 cacctgggcc tcggaggcgg agtactgcgc cttgatgacc tccttcttgg tgcggaaggc
    10921 ctcgatcttc gagcgcagct tcttctcgtt ggcgatgagc tgctcctgct ggccctcgag
    10981 ctccttgatc tgcgtgtcga gcgactggag ctcgctctgc gcgagcgtct tgcgctcgag
    11041 cgccgtgcgg gcgaggtcct cgcggccggc cgagagcgcc tgacgcgcct gcgtgtcgag
    11101 cttgacgacc gactcctcga gcttcttgga ctgcatctgg agccgcttct tcgacgtgac
    11161 gacgtcggcg atccccttct tcacgttctg aagaagctcg acctgcttct ggtagccgta
    11221 gtcgagcgtc tcgcccgggt cctcggcgcg atcgaggagc ttcgagatct tcgacttcac
    11281 gacggtggac atgcgaccgg cgaggcctgg catgccgcct cctttttctt ccgttgcctg
    11341 taggtcggcg aagactaacg ggcggccacg gttggcgcgg gcgctcccgc gtcagccggg
    11401 cgcggcccag tcagcggtgc ccgttgaacg agatgacctg ctggatgccg ctcgggcggt
    11461 tcgtgtagtc catgtcgatg agcggcagcg ggctgaactt gatctgggtc tgcgcgcgcg
    11521 gctggcgcca tgtcgcgggg tccggaccga acttctcggc gagcgaggtg ccggcggcgt
    11581 cgatcgcctt ccacagcttc gtggcgcacg ccttcgccga cttggcgcag tagtgctgcg
    11641 agaacgcccc cctcaccttc gcgccgagca ggctgcggaa gtccttgtcc atgtagtggt
    11701 gccagccgct gtactggccg cccggcgggg tggcgaagcg cccgtgcagc gtgtcggcga
    11761 gctgctcgca gagcggcttg ccgatcgcgt cgcacagaga ggcgtcggcc acctggcgcc
    11821 agatgccgtc catgatcggc acgccggcgt cgtccatgtt gccgtcgcgg tcctggtcga
    11881 tccgcggcgc tcgccgcgcg ttccaggcgt cgagctgggc caccatcgcg gccgcgcgcg
    11941 ggctcggcga gtccaccttg ctgagcatcg cgcgcatggt cggccagaac aggttgccgc
    12001 gcgggtcctg caccgccgcc gcgttcatgg ccgccgtgac cgacccgagg gagtgcttat
    12061 cggtgcgcgc gagctcgcgc tgcagcaggt cgttgcgcgg cacggggccc tcggacccga
    12121 agcggtcgtc gccgctcacg aagccgcgcg ccggccggtt gttccagttg acgatcagcc
    12181 cgttatcggg gttcgacccg cgcgggtgct gcgaggccct gaggtacccc ttccactcgt
    12241 agccgccgcg gccgtcgttg acgaggctgc cgtcggcccc cttgggccgg agcggcagca
    12301 ggccggtcgt gtagaggccg gtgttctggt ggtcggcgta gaacgcgttg aaggtctgag
    12361 gcgtctgggc ggcggaggcg gcgaagtcgt cgtacgactt gaccctgccg aaggtgaggt
    12421 cctggaagaa gagctgatcg agcgtgtcct tgccgtagga cgagcgcctg cgcgagatcg
    12481 cgaccgtctt cccgccgctc gtcttcgcgt agccgacgac cggaccgtgc acggtctggc
    12541 ggaagcggac gacccgcttc gtggtgccct tggtcagcgt gccggcgttg accaggtcca
    12601 tgctgcggca cctgcccttg tagaggtact tcgtcttgct gccgccgcag agcgtctccg
    12661 cgtaggtgtc aatgatgtcg ccgccggccg acgtgagggt ccacgcgaag tcctccccgc
    12721 gcccgatcag catgtagccc gggaacggca ccgacgtggc gccgcgcacc tggatgtcgg
    12781 gcccgtagag gcccatctcc gccacgaggc cggggaagaa gtagctgatc tgcggaccgc
    12841 ccacgaacag cggatggccg gtggccgagc gctgcccgct caccatcagg acgttgctcg
    12901 cctccgcgcg ggcgctcgac gcgccggaca gcccgccctc gcccttgaag gagccgttgc
    12961 gcagcgtgac cgtgcccttg cgcgagcccg tggccgtgtc gtacggggcc ttgttgcgca
    13021 gcgtggcctt ctgctcgggg tccttgcggt tgcgcaggtc ctgccagatc gcgaagccgc
    13081 gctttgcccc gaacttgcgc tggagcccgt cgaggagctg gctcgtggcc ggctcgctgc
    13141 cgccgccctc gccgaggaac tggcccttga tcgcgttgaa cgcgtagatg tcgttgcgcg
    13201 tgaacttggg cgacgacggc gagttggccg cgtaccaggc gttgatgccg cccacgtaga
    13261 ggtcgatgtc gtgcaggagc tgcacgccct tcggccccgc ctgctggatc accttggtct
    13321 gccgcgcgac gatcgcgttg gcctgtgcgc tcggctcgaa ctggtacagc tcgcggatga
    13381 ggtcgatcgc cgacaggttg ggcgcgtcga ccgcggcggt gatgccgttg aagcggccct
    13441 ggttgagcag caggctgcgg tcggacgcga tggcccagcc ggcgccgaag atcacgtcgt
    13501 cgttggtctg cccgtagatg tgcgggacgt tgtactcgtc gcgcttgatg aggacgccgg
    13561 ggcgccccgg gatcgtctcc tcgcgcacga cggtggtcgg cccgagcgcc tcggacttga
    13621 agaagctcgg caggtccgcg tccgtgacgt tgtcgtagcg cggcgtgagc gcgttgtaca
    13681 tctgcgcctg cttgtcggcc tcgggcccgg gcgtctggta ctggcccgac gggaggatgt
    13741 tgagcgcggt gccggcgtag tcggcggcca gggcggccgg ggcagcggcg agggcacaca
    13801 gcgcggcgac tacgagggcg agggtgagac ggctcatgcg acgtctggca cgttacccga
    13861 cgcgggcgac gtcacgcgcc tccgcccggt cgtggtcgac ccccgcccgc cagaagaaga
    13921 acgccagagc actcagcatg atcagcaggt cgacgaccat catcagcccg cccgcgagct
    13981 gctggtcggc gagcgcgctg atgccgtgcg cgccgctgta gccgtcgtac gcgggcgttc
    14041 gctggacgat gaacgccatg ccgacgaaca tcgtcagcac gcgggtgccg agcacgaagc
    14101 cggccttcca cagctccccg ggcaggcgcg agcggttcgg ctcgagcacg ggccaccaga
    14161 cgagcagccc gatcgcgacg aacgactggt gctggagccc gtgcacgacg tcgttgccaa
    14221 gcgcggcctc gaacgcgaac ggcaggtgcc acgtgtagag caccgtgatg tagaccccca
    14281 gcgcgacgag cggctggcgg acagagcgcg agagcgcgcg cagtcgcggc tggcgcgcga
    14341 gcgcgacgag cagggaccgc ggcggcatga agaacagaac cggcgagcgc gccccgacga
    14401 gcagcagcgg cccggccagg tcggcgatca gcaggtgctg gcccatgtgg gcgacgagca
    14461 ggtcctcgcc gagccggtcg ggcgggccga acaggccgag cgctatgagc cccacgccgc
    14521 cgtaccaggc ggccacctgc ccgcgcggga cgcggtggcc gcggcgagcg agcctgcgcg
    14581 tcgcgcgcgc gtgcagccac acgagcagcg cgacgacgac gaggccggcg gggtcgagcg
    14641 agatcacgtg cggggattgt gacccgcgcc ggggcgcggg tcacgcacgc cgcaccgcgc
    14701 ggctaggccg cgctggtcac ggcgtcgatc gcgtccccga tcacgccacc cgtggacgcg
    14761 ccgggcgcct gctccaggat ctcctgggcg gtgttgatcc gctcctggta gtcctggcgc
    14821 tgcatgcgcg cgacgaccag ccgctgcatc tgggccgtgc cctcgaagat ctggtagatc
    14881 ttggcgtcgc ggaaccactt ctcgagcggg cattcggtgg tctgcgcgta ctcgccgacg
    14941 aggtccatca gcgtggtggt ggcccacatc gtcacgtcgc ccgccttgag cttggacatc
    15001 gagccctggc cgccggtcat cggcacgccg ttgcgtccca tccaggccgc gcggtagacg
    15061 aggagccggg ccgcgtcgat ctcggtcgcc acgtcggcga gcacctgctg gtggcgctgc
    15121 tggtcgagca gcgggccgtc ggcgtcggac ttctcctcga ggtagtcgag cgtccactcg
    15181 tacgccgcgc gcgcgatgcc gagggccgcc gcgccgacga gcgggcgcgt gatctcgaac
    15241 gtggcgagcg cgccggactg cttgccggtg gcctggcccg accgcgcacg ctcgagcttc
    15301 gcctcgagct tctcgatccc gccgagcagg tactccatcg ggagccggca gttgtcgagc
    15361 acgacctcgg cggtctgcga ggcgcggatg ccgatcttgt cctccttctt gcccatggag
    15421 aagccgggcg tgtccttcgg gacgatgaag gaggcgtggc cgcggtggcc gagcgtcgcg
    15481 tcgacggtgg cgacgacgac ggtgacgtcg gcgatcccgc cgttcgagat gaacaccttg
    15541 gtgccgttga ggacccactc gtcgccgtcg cgcttggccg tcgtgcgcag cgacttgacg
    15601 tcggagcccg cgccggcctc ggtcacggcg taggcgccga gcttcatgtc ttccttgctc
    15661 gtcccgtaga tctcggggat ccaccggccg atctgctcag gggtgcccga ggccgcgacg
    15721 ccggcggcgg ccagcgagga ggcggagatg gcgagcgcga tgccggcgca gccccagtgc
    15781 agctcctcgg cgtagatcac gccgaacagg ccgtcggggt cgttggccat cttctggatg
    15841 tagtcgaggc cggagaggtc ccacttgcgg gcctccttca tgacctccca cggcacgctc
    15901 tgctcgcggt cgtgcttgtc ggccaccggc cgcatgtgct cttcagcgaa gcgatggcag
    15961 tgagcctgcc actccttctg ttcgtcggtg agccgaaagt ccacggcgta cgtgcctccg
    16021 tcatagttga ctgaccagtc aatcgtatcg gacaccaaat cgtgaagagg tcttcaggct
    16081 cgctcggtat cgtgccgtcc gtgccccggc tgaccctcct cgtggccgcg gccctgttcg
    16141 cgctcacggc gctcgggggc tgctcgctca agtccgaaga ctccggtggc gacccggcgc
    16201 agcgcgtcgg cgagggagtg agctccggcg acgactccgc ggtcgaggcc ctcggcttcc
    16261 ccaccaccgc cacgcgcgac acgatccgcg tacccgggag cgacccggcg gccgacgcgg
    16321 ccggcgtggc cagcgcgctg ttccccgcca cgaccaccga aacgcggccg ccggcggtcg
    16381 cgctcgtcga cggcgacccg tggcaggccg gcgtggccgc ggccgtgcgc gcgggcgccc
    16441 cgctgcgcgc cc
//
LOCUS       DASVPL010000062         7854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_124819, whole genome shotgun sequence.
ACCESSION   DASVPL010000062 DASVPL010000000
VERSION     DASVPL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124819"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF517_03110"
     CDS             <1..258
                     /locus_tag="VF517_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8051953.1"
                     /translation="VAVSATGNGEAMMRMVAAHEIASLVRHAGLTLGAAADRVMALDG
                     PDGGLIAVGRDGALAMPFTTAALYRGWGRGAEPVRTGIGAD"
     gene            complement(255..779)
                     /locus_tag="VF517_03115"
     CDS             complement(255..779)
                     /locus_tag="VF517_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051954.1"
                     /translation="METYFRVEPGDRDPEAMLDPDNQETEPWSGTVQGRCDKCGGSGE
                     TEHECESCTDDQADPDCPACGGKVRYTDECPACEGSGEIDDSCRDGVSVFPDEDGLYR
                     YMLKRDADLDERCQLVKLEGEQSQDEDFDADEGALLVKPRRIVASGKLDWDRIEELRS
                     ELSANGELSGGDGG"
     gene            complement(832..1137)
                     /locus_tag="VF517_03120"
     CDS             complement(832..1137)
                     /locus_tag="VF517_03120"
                     /inference="COORDINATES: protein motif:HMM:NF025590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEX8051955.1"
                     /translation="MSPRVPAAAALAALALAGCGGGDDDNDTKVLDVRPVERGIARGV
                     EKDNPKTDVVAVECPDDVEQRKGVVFKCRVRGSREGEQAEATVTQVDDEGRVRYVVP"
     gene            complement(1134..2579)
                     /locus_tag="VF517_03125"
     CDS             complement(1134..2579)
                     /locus_tag="VF517_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051956.1"
                     /translation="MRIAARARARWDGLGRPERIALAGLALLLAGGIALRVVAMYAYR
                     PAFIGYPDTREYALGADGDLWADPFRTVGYPLFLRALHAFTDHLSFVVVVQHLLGVAT
                     ALLLFAAVRRCGGPAWLGLVPAAVVLLGGDHVLFEHAVLTESLYTFLTAAALYAGVRC
                     LDGAAFGWTAAAGLLLGLAATVRIAGLVLVPLLAAWMLLVPSTRRRERALHAGVAAVA
                     GGLVLLGYLLAAHAGTERWSFARTGAYNFYGRVSSFADCERFDEPAGTEVLCDRTPPH
                     ERRAPQWYIFTGPAATELGYPEDEVPRATVERISLFARRAALAQPLDWLDAAARDFAR
                     YVVPDAFGRPSNTPTARDYRRLLNHPIGIPTNYHAVDAYWTIPGVRNRAPLFGFLKDY
                     EAVTAVEGAPIGIMLLLTLGAPIVLRGRERRGALLLGGIALAILVVPVTTINYDGRLG
                     VPAYGPLAAAAALGGLGGVRRVRARLPSSRS"
     gene            complement(2576..3340)
                     /locus_tag="VF517_03130"
     CDS             complement(2576..3340)
                     /locus_tag="VF517_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8051957.1"
                     /translation="MPGETTDRSGVLSDRITPTDPDPVVSFVVPAYNEAANIEDVLRR
                     VYALPLRAEVIAVDDASSDRTPEILASWAESHGLTAIRHGRNSGKGAAVRSALPHTTG
                     DIVVIQDADMEYDPADVVDLIRPIVQGYADVVYGSRLTGGRPQRAYMFWHLVGNRILA
                     LITNVLWNTTLTDMETGYKAFRGEIIRSLTLEESDFRIEPEITSEILRRKLRLYQIPI
                     AYYGRTPEEGKKISWRDGLPAVWTLVRLRFTRGRGA"
     gene            complement(3364..3780)
                     /locus_tag="VF517_03135"
     CDS             complement(3364..3780)
                     /locus_tag="VF517_03135"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX8051958.1"
                     /translation="MASERKHKLSTFVSTKLFNPGVRFMLDRGIAPKSVALLETTGRK
                     SGEPRRTPVGNGLRDGHFWIVTEHGRRAAYVKNIEADPRVRVKVGRRWYSGTAHVLPD
                     DDPLERMRILGRAANDSMVRLMGTEHLTIRIDLDAS"
     gene            complement(3785..4480)
                     /locus_tag="VF517_03140"
     CDS             complement(3785..4480)
                     /locus_tag="VF517_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX8051959.1"
                     /translation="MKLVLWDIDGTLVDSAKLGRDAFFEAFEQVIGHTPTTIVPFAGR
                     TDLEIAYDMFETEGVEAAPETLLDDFEEALVAAMAARLDELERIGRALPGTSEALERL
                     HGEPGVIQSLLTGNVERNAMLKLKPFGLARWLEFGCGGYGSDHRVRGELVAIARARAE
                     RAHHARIDPASVVLIGDTPLDVVAAREGGARAVGVATGPYDEQALADAGADAVLPDLS
                     DTERVVDAVLGNH"
     gene            complement(4477..5109)
                     /locus_tag="VF517_03145"
     CDS             complement(4477..5109)
                     /locus_tag="VF517_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051960.1"
                     /translation="MTPVTIESITVADPPQAWAEAGFRVDGETCRIGSIDVRLAGREA
                     GKGLVGWSLRGIAGTELDGLPTTAATGAPDPRVASRAPHPNGALRIDHVVAFTPDRAR
                     TVAALEAAGLDLRRLRDEPTPAGGGFQAFFRLGESILEVVEAPPGSPVPPDAPARLWG
                     LAFLVDSLDQTQRLLGDRLGEPRDAVQPGRRIATLRKSAGLSFGCAFMSG"
     gene            5164..5976
                     /locus_tag="VF517_03150"
     CDS             5164..5976
                     /locus_tag="VF517_03150"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8051961.1"
                     /translation="MPDATEFDRATAVRATGDGVYEAAVDERWNVMRGPHGGYIAAML
                     LRALTDAVGDEARVPRSLTVYYPAAPKHGPVTIRCQVERAGRSMTTVSARLEQDGKAA
                     ALAVAAFSAEWPRTVEFDDTQRPEVPEPEAIEPLDRGGRVPPFTRYFDFRPAVGERWF
                     SGAERALSGGWMRLNDDHALDGPLVAALADAWPPAIFPVATDFVAAPTIELTVHFRAA
                     LPVAGDWVLGLFESKVGRDGFFEEDGTLWTRSGDLIAQSRQLALAIDMGAAS"
     gene            complement(6062..>7854)
                     /locus_tag="VF517_03155"
     CDS             complement(6062..>7854)
                     /locus_tag="VF517_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8051962.1"
                     /translation="VGVAAASGADVDAAARERLSELVSDLRSLPERQRSALVLRELSG
                     LDYEELGAALDISPNAARQTVFKARVSMQANVEGRTAHCDEVRAAMSKNDGRFRRRRS
                     IQAHLERCPVCTEFATGLEERPRDLAALFPPLPGGVAAAMLAAASGGAGAASSAAGGA
                     GASGAASSAAAAAGSGGAASSGAAGAADAASSGAAGASGAASSGAAGAASSGSAGAAS
                     SGSAATGASPRSEDRPRRRALVLALLLLLLVGGTVATALLANGGDEGSDSPGEVAQQP
                     AAGEGDAGSNGSGGGAGDDGAGSGLQPDATDDEKDTGSARDRGDGGDTSESGSDSDSE
                     SDSGSGAAPGSGSGDSSDSDSGDADSGDSGSGDDSASGSAPKSSGSGGSRDDRSSEDT
                     PARTRECVERTVTRRSSPAQDAYSNRGGRVEVQIREDCTPTRAAQDCVGSGSASDSGS
                     GANGGSEGSGSGGDGSGSGAGSGSGEGSDDGSTSGDGSGSGDRSGSGSGGEDGSGSSS
                     GGDGSGSGDGTTSGDGSASGGGDDSGSGAGSGSGSGGSRDNAGASGSSPCARATSDGL
                     PITGLELGLIAGCALLLLGLGLALRRVASA"
BASE COUNT         1016 a         2823 c         3010 g         1005 t
ORIGIN      
        1 gtcgccgtct cggcgaccgg caacggcgag gcgatgatgc gaatggtggc cgcgcacgag
       61 atcgcatcgc tcgtgcgcca cgccgggctc acgctcggcg cggccgcgga ccgcgtcatg
      121 gctctggacg gtcccgacgg cgggctgatc gccgtcggcc gcgacggcgc gctcgcgatg
      181 ccgttcacca cggccgccct ctaccgcggc tgggggcgcg gggccgagcc ggtgcggacc
      241 gggatcgggg cggactagcc gccgtcgccg ccggagagct ctccgttcgc gctcagctcg
      301 ctgcgcagct cctcgatgcg gtcccagtcg agcttgccgc tcgcgacgat ccgtcgcggc
      361 ttcacgagca gcgcgccctc gtcggcgtcg aagtcctcgt cctgcgactg ctcgccctcg
      421 agcttcacga gctggcagcg ctcgtcaagg tccgcgtcgc gcttgagcat gtagcggtag
      481 agcccgtcct cgtccgggaa gacgctcacg ccgtcgcggc aggagtcgtc gatctcgccg
      541 ctgccctcgc aggccgggca ctcgtccgtg tagcggacct tgccgccgca cgcggggcag
      601 tccggatcgg cctggtcgtc ggtgcaggac tcgcactcgt gctcggtctc ccccgacccg
      661 ccgcacttgt cgcagcggcc ctgcacggtc ccgctccagg gctcggtctc ctggttgtcg
      721 gggtcgagca tggcctcggg atcgcgatcc ccgggctcga cgcggaagta ggtctccatc
      781 gcgggcgagc ataggcccgg ccccggggca cgacccggcg ccgccgcggc cctaggggac
      841 cacgtaccgc acgcgcccct cgtcgtccac ctgcgtcacg gtcgcctcgg cctgctcacc
      901 ctcgcgcgag ccgcgcaccc ggcacttgaa cacgacgccc ttcctctgct ccacgtcgtc
      961 ggggcattcg accgcgacca cgtcggtctt cgggttgtcc ttctcgacgc cgcgcgcgat
     1021 cccgcgctcg accgggcgga cgtcgaggac cttggtgtcg ttgtcgtcgt cgccgccccc
     1081 gcaaccggcg agtgccaggg cggcgagcgc ggctgcagcg ggcacgcggg ggctcacgag
     1141 cgcgatgatg gcagccgcgc gcgcacccgg cgcaccccgc cgagaccccc gagcgcggcc
     1201 gccgcggcca gcggtccgta ggcgggcacg ccgagccgcc cgtcgtagtt gatcgtcgtc
     1261 acgggcacca cgaggatcgc cagcgcgatg cctccgagga gcagcgcgcc gcggcgctcg
     1321 cgcccgcgca ggacgatcgg ggcgccgagc gtgaggagga gcatgatccc tatcggcgcg
     1381 ccctccaccg cggtcacggc ctcgtagtcc ttcaggaagc cgaagagcgg cgcgcggttg
     1441 cgcacgccgg ggatcgtcca gtaggcgtcg accgcgtgat agttggtcgg gatcccgatc
     1501 gggtggttca acagtcggcg gtagtcgcgc gccgtgggcg tgttgctcgg ccgcccgaac
     1561 gcgtcgggca cgacatagcg ggcgaagtcg cgcgcggcgg cgtcgagcca gtcgagcggc
     1621 tgggcgagcg cggcgcggcg cgcgaacagc gagatccgct cgaccgtggc gcgcgggacc
     1681 tcatcctctg gatagccgag ctcggtggcg gccgggccgg tgaagatgta ccactgcggc
     1741 gcgcggcgct cgtgcggcgg ggtgcggtcg cagagcacct cggtgccggc cggctcgtcg
     1801 aagcgctcgc agtcggcgaa gctcgacacg cgcccgtaga agttgtaggc gccggtgcgc
     1861 gcgaacgacc agcgctccgt cccggcgtgg gcggccaaca gatagccgag cagcacgagg
     1921 ccgcccgcca cggcggccac tccggcgtgc agggctcgct cgcgccggcg ggtcgatggc
     1981 acgagcagca tccaggccgc gagcaggggc acgagcacca gcccggctat tcgcacggtg
     2041 gcggccaggc cgagcagcag cccggcggcg gccgtccagc cgaacgcggc cccgtcgagg
     2101 cagcgcacgc ctgcgtacag cgcggcggcg gtgaggaagg tgtagagcga ctcggtgagg
     2161 acggcgtgct cgaacagcac gtggtcgccg ccgagcagca ccacggcggc gggcacgagc
     2221 ccgagccacg cgggtccgcc gcaacggcgc acggcggcga acaggagcag cgcggtggcc
     2281 acgccgagca ggtgctggac caccaccacg aacgacaggt ggtcggtgaa cgcgtgcagc
     2341 gcgcgcagga acagcgggta gccgaccgtg cggaacgggt ccgcccagag gtcgccgtcg
     2401 gcgccgagcg cgtactcgcg cgtgtcgggg tagccgatga aggcgggccg gtacgcgtac
     2461 atggcgacca cgcgcagggc gatcccgccg gcgaggagca gcgcgagccc ggcgagcgcg
     2521 atgcgctcgg ggcggccgag accgtcccag cgcgcccgtg cgcgcgcggc gatcctcaag
     2581 cgccgcggcc gcgggtgaag cgcagccgca cgagcgtcca gaccgcgggc aacccgtcgc
     2641 gccacgagat cttcttaccc tcctcgggcg tgcggccgta gtaggcgatc gggatctggt
     2701 agagccgcag cttgcggcgg aggatttcgg acgtgatctc gggctcgatc cggaagtcgc
     2761 tctcctcgag cgtgaggctg cggatgatct cgccgcggaa cgccttgtag ccggtctcca
     2821 tgtcggtgag cgtggtgttc cagaggacgt tggtgatcag cgcgaggatg cggttgccga
     2881 ccaggtgcca gaacatgtac gcgcgctgcg gccgcccgcc ggtgaggcgc gagccgtaga
     2941 ccacgtcggc gtacccctgg acgatcgggc ggatgaggtc caccacgtcg gcggggtcgt
     3001 actccatgtc ggcgtcctgg atcacgacga tgtcgccggt cgtgtgcggc agcgcgctgc
     3061 gcaccgccgc gcccttgccg gagttgcgcc cgtggcggat cgcggtgagg ccgtggctct
     3121 cggcccagct cgcgaggatc tcgggggtgc ggtcgctcga cgcgtcgtcg acggcgatca
     3181 cctcggcgcg cagcggcagc gcgtacacgc gccggagcac gtcctcgatg ttcgccgcct
     3241 cgttgtacgc cggtactacg aagctgacga cggggtcggg gtcggtcggg gtgatgcggt
     3301 cgctgaggac gcccgacctg tctgtcgtct cccccggcat cggcgcgggc gaagggtagg
     3361 gcttcagctg gcgtcgaggt cgatgcggat ggtgaggtgc tcggtgccca tgaggcggac
     3421 catcgagtcg ttggcggcgc ggccgagaat gcgcatgcgc tcgagcgggt cgtcgtccgg
     3481 aaggacgtgg gcggtgccgc tgtaccagcg ccggccgacc ttcacgcgca cgcgcgggtc
     3541 ggcctcgatg ttcttcacgt aggcggcgcg gcgaccatgc tcggtcacga tccagaagtg
     3601 gccgtcgcgc aggccgttgc ccacgggggt gcggcgcggc tcgccggact tgcgcccggt
     3661 ggtctcgagc agggccacgc tcttgggcgc gatcccgcgg tcgagcatga accgcacgcc
     3721 cgggttgaag agcttcgtgg agacgaaggt gctgagcttg tgcttgcgct cggaggccat
     3781 gccctcagtg gttccccagg acggcgtcga ctacgcgctc ggtgtcggac aggtcgggga
     3841 ggaccgcgtc ggcgcccgca tcggcgagcg cctgctcgtc gtacggcccg gtcgcgacgc
     3901 cgaccgcccg ggcgccgccc tcgcgcgcgg cgaccacgtc gagcggggtg tcgccgatga
     3961 ggacgacgct cgcggggtcg atccgggcgt ggtgcgcgcg ctccgcgcgg gcgcgggcga
     4021 tcgccacgag ctccccgcgc acgcgatggt cggagccgta gccgccgcag ccgaactcga
     4081 gccagcgcgc gagtccgaac ggcttcagct tgagcatcgc gttgcgctcg acgttgccgg
     4141 tcaggagcga ttggatcacg ccgggctcgc cgtgcaggcg ctcgagcgcc tcggacgtgc
     4201 cgggcagcgc gcggccgatc cgctcgagct cgtcgaggcg ggcggccatt gcggcgacga
     4261 gcgcctcctc gaagtcgtcc agcagtgtct cgggcgcggc ctcgacgccc tcggtctcga
     4321 acatgtcgta ggcgatctcg aggtcggtgc ggccggcgaa cgggacgatg gtcgttggcg
     4381 tgtggccgat tacttgttcg aacgcctcga agaaggcgtc gcggcccagc ttcgccgagt
     4441 cgacgagcgt gccgtctatg tcccagagga cgagcttcat cccgacatga aagcgcagcc
     4501 gaacgacagg ccggcgctct tgcggagggt ggcgatgcgg cggcccggct ggacggcgtc
     4561 gcggggctcg ccgaggcggt cgccgagcag gcgctgggtc tggtcgaggg agtccacgag
     4621 gaaggcgagc ccccacaggc gcgctggggc atccggcggt accggggatc cgggcggggc
     4681 ctcgaccacc tcgaggatcg actcgccgag gcggaagaag gcttggaagc cgccgcccgc
     4741 gggagttggc tcgtcgcgca ggcggcgcag gtcgaggccg gcggcctcga gcgccgcgac
     4801 ggtgcgggcg cggtcgggtg tgaaggcgac cacgtgatct atgcggagag cgccgttggg
     4861 gtgcggggcg cgagacgcga cgcgcgggtc cggtgcaccg gtcgcggcgg tggtcgggag
     4921 cccgtcgagc tccgtgccgg cgatcccgcg cagtgaccag ccgacgagcc ccttcccggc
     4981 ctcgcgcccc gcgagccgca cgtcgatcga cccgatgcgg caggtttcac catccacgcg
     5041 aaagccggcc tcggcccagg cctgcggcgg gtcggcgacg gtgatcgact cgatcgtgac
     5101 cggggtcatc tgtggtgcac cacacgggca tcttcgccgc tcgcccttag gatcgcccgt
     5161 cccgtgcccg acgcgaccga gttcgaccga gccaccgccg tccgcgcgac gggcgacggg
     5221 gtctacgagg cggcggtcga cgagcgctgg aacgtgatgc gcggcccgca cggcggctac
     5281 atcgcggcga tgctcctccg cgcgctcacc gacgcggtcg gcgacgaggc gcgggtgccg
     5341 cgctcgctca cggtctacta cccggccgcg ccgaagcacg ggccggtgac gatccgctgc
     5401 caagtcgagc gggcgggccg gtcgatgacc accgtgtcag cgcggctcga gcaggacggg
     5461 aaggcggcgg cgctggccgt ggcggcgttc tcggccgagt ggccgcggac ggtcgagttc
     5521 gacgacaccc agcgacccga ggtgcccgag cccgaggcga tcgagccgct cgaccgcggc
     5581 gggcgggtcc cgccgttcac ccgctacttc gacttccgcc cagccgtcgg cgagcggtgg
     5641 ttctcgggtg ccgagcgggc gctgagcggc ggctggatgc ggctgaacga cgaccatgcg
     5701 ctggacgggc ccctggtcgc cgcgttggcc gacgcctggc cgccggcgat cttcccggtg
     5761 gcgacggact tcgtggcggc gccgacgatc gagctgaccg tgcacttccg cgccgcgctg
     5821 ccggtcgcgg gcgactgggt cctcggcctg ttcgagtcga aggtcggccg cgacggtttc
     5881 ttcgaggagg acggcacgct ctggacccgc tccggagacc tgatcgccca gtcccgccag
     5941 ctcgcgctgg cgatcgacat gggcgcggcg tcgtaggcgc gccgctccgc gcgaggtgcc
     6001 cgcgcgaccc gaagtcaaat gcgtcccgaa tagggacgtt tctgacttac ccctcgccgc
     6061 gctaggccga cgcgacgcgg cggagcgcta ggccgaggcc gagcagcaga agcgcgcagc
     6121 cggcgatgag gccgagctcg aggccggtta tggggaggcc gtcgctcgtg gcgcgggcgc
     6181 aggggctgga gccggaggcg ccggcgttgt cgcgcgagcc gccggacccg gagccgctgc
     6241 ccgcaccgct gcccgagtca tccccgccgc cggaggcgga gccatcgccg gaagtggtgc
     6301 catcgccgga gccggaaccg tcgccgccgg agctggagcc ggagccgtcc tcgccgcccg
     6361 aaccggaacc ggagcggtcg ccggaaccgg aaccgtcgcc ggaagtggag ccatcgtccg
     6421 agccctcgcc ggagccggag cccgcgcccg agccggaacc gtcgccgccc gagccactcc
     6481 cctcactgcc gccgtttgcg ccggacccgg aatccgacgc cgagccgctg cccacgcagt
     6541 cctgcgcggc tcgggtcggc gtgcagtcct cgcggatctg gacctcgacc cggccgccgc
     6601 ggttcgagta agcatcttga gccggcgagg agcgccgggt gaccgtgcgc tcgacgcact
     6661 cgcgggtgcg ggcgggcgtg tcctcggagg accgatcgtc gcgcgaaccg ccggagccgg
     6721 aggacttggg tgcggagccg gaagcggagt cgtctccgga accggagtcg ccggagtcgg
     6781 cgtcgccgga gtcggagtcg gaggagtcgc cagagccaga gccgggcgcg gcgcccgagc
     6841 ccgagtcgga ctcggagtcg gagtcgctgc cggactcgct ggtgtcgccg ccatccccgc
     6901 gatcgcgcgc cgagccggtg tccttctcgt cgtcggtcgc gtccggctgc aagccggagc
     6961 ccgcgccgtc gtcgcccgcg ccccctccgc tgccattcga gcccgcgtca ccttcgccgg
     7021 cggccggctg ctgggcgacc tcgccggggg aatcggagcc ttcgtcgccg ccgttcgcga
     7081 gcagggcggt cgccaccgtg ccaccgacga gcaggagcag gagcagcgcg agcaccagcg
     7141 cgcgacgccg agggcggtcc tcgctgcgcg gagaggcacc ggtcgcggcg gagccggaag
     7201 acgccgcgcc agcagagccc gaagacgccg cgccagccgc gccggaggaa gccgcgccgc
     7261 tcgcgccggc cgcgcccgag gaggcggcgt cggccgcgcc ggccgcgccg gaggaggcgg
     7321 cgccgcccga gcccgccgcc gccgccgcgg acgaagccgc tccgctcgcg cccgctccgc
     7381 ccgccgcgga cgaggccgct ccggcgccgc cggacgcggc cgccagcatc gccgccgcca
     7441 ccccgccggg cagcggcggg aagagcgccg cgaggtcgcg cgggcgctcc tcgaggccgg
     7501 tcgcgaactc ggtgcagacc gggcagcgct cgaggtgggc ctggatcgag cgccggcggc
     7561 ggaagcggcc gtcgttcttc gacatcgccg cgcgcacctc gtcgcagtgc gcggtccgcc
     7621 cctccacgtt cgcctgcatc gacacgcgcg ccttgaagac cgtctgccgg gccgcgttcg
     7681 gcgagatgtc cagcgcggcg ccgagctcct cgtagtcgag cccgctcagc tcgcgcagca
     7741 cgagcgccga gcgctgccgc tcgggcagcg agcgcaggtc ggatacgagc tcggacagac
     7801 gctcacgggc ggcggcgtcc acgtcggccc cggaggcggc cgcgaccccc accg
//
LOCUS       DASVPL010000063         9784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_321061, whole genome shotgun sequence.
ACCESSION   DASVPL010000063 DASVPL010000000
VERSION     DASVPL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321061"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VF517_03160"
     CDS             complement(<1..480)
                     /locus_tag="VF517_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051963.1"
                     /translation="MLNRLTAPHGSNLGDPGRLIAHVTAIFMLGAGIIHLSAASDHEA
                     LPLMMVGFLIVALLQIAVGGLLLAGRPRRLLLWGAVALTVSSLGLWFMSRTMGLPFLP
                     DGHMEPIGFKDGVTVVFQLATLPGLFLLMNRHLAGMVVPAAFGSQAMSVMGVGATLLM
                     "
     gene            complement(593..1447)
                     /locus_tag="VF517_03165"
     CDS             complement(593..1447)
                     /locus_tag="VF517_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051964.1"
                     /translation="MVVGGALRHGSFARAAVAAAVAVLACLAASPASAQTTQTTSGTV
                     RDCHEIVALVNVPKGPVREQVPAQYTLYEETPGQATVYLRGSKCAGVDLSGREIPTYM
                     ANFGAVIESPDHSGCMSHGERDFGFEAFQGDYVKYCTFYPFYVATDNREWARWLRADG
                     TSDYPVYVTRDWEWDLRPGLVPGQFGFRFRVGGSVPSPFELEGSGIEREGTIPLQITY
                     WYPSGDKQVKLYAENDLKLGHATGEVRTPAGTDLARMLGATSSSFASGFGLNYFAQAL
                     LVKEIRSP"
     gene            complement(1607..2155)
                     /locus_tag="VF517_03170"
     CDS             complement(1607..2155)
                     /locus_tag="VF517_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8051965.1"
                     /translation="MARLPYVDPEDASDPVREALERVPPINIFRMVANAETAFRPWLA
                     FGGALLSSLELDARVRELVILHVGRLSGAEYEWAQHVPIALAVGATRDEVAAVERGDL
                     SAFGESDRAVLRFTEEVVREVRASDETLAEVRRSLSPREVVELLLVIGQYMLVARVAE
                     TAGIEIDEGGTAVADAAVRRPR"
     gene            2316..>9784
                     /locus_tag="VF517_03175"
     CDS             2316..>9784
                     /locus_tag="VF517_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051966.1"
                     /translation="MLAAAFAASSAHAATPPPNDNFASAELLSGRSASAGGTTDGATS
                     EPGEPYDYWYGKTIWYRWVAPADGTVAADVGASAIPAVVTAFTGSSVGALTRVPSPET
                     NGSTSKRARFTVSAGTTYHLRVESGSYDRNGPVAIALELRDPPPNDRFASPQVLTGDE
                     ATATGDTSWATRETGEPRAASGSGGGTVWYSWTAPSTGGVTLSTEGSAIDTVLGVYTG
                     AALSSLVQVSANNDARSGVRTSRVTFRAEAGTTYRFVVDGYSSGQVGAVRLALSLRPP
                     PANDAFAGATVLDSARDLTVIGNNNGASAELGEPQHYSYYAPKSSVWFRWTAPSTGSL
                     TIRATTDFAPVLAAYTGNSVAALTRVPNQPQQWNGGPEQIRLRVNAGETYYIALDSYV
                     VHGDFSLNLALIDNPGNDDLADAVELEGAFVEVTSDNVGATQEPCEPRHDDNYYDPSV
                     WYEWTAPADGGVVVDTSGSDLLTAVGIYTGDSLCSLTRLPVTRLSATGTPAVRTFTAK
                     GGTTYRIAIDGQAARMGRFRLSLRLTPPPDNDMFAGAQPLAGPSASAAGNTLGASAEA
                     GEPAANGPSSVWFEWTAPSSGRTTLAALRATGELAVYTGDAVGSLSAVYTRGYRSQLV
                     FHARAGVTYRIALHGGAGAAQGPYELGLTHVTGPANDDFADATPLPATDATVVGSTAG
                     ATRELNEPRLDYDYAERSIWYSWTATADANVVLDTYGSGFRTGMGVYTGESAAALVRV
                     AGHYGSDKPARVGFRATAGTTYRIKIDGAYEADFGDVKLALAVRGNPPNDNIADAKSL
                     PSAPAVSIAGWNAGATAETGEPTHNSYWSANQSVWYRWIAPRTGSLTIRSDAHFQTVI
                     AAYTGSVIGSLSRVANQPQDWNGGKEQIRIRVEAGKQYMIAVDGDTSGYRGYGDFQLS
                     LQLAQGPPNDEFSSPLPLSGALTELVGSTVHATQEPCEPVHDGNYYDPSVWYEWTAPE
                     TGPVTIDTTGSDFQTVVAVYTGDVLCTLARVETLAFSTGTQPAKRGFRAKAGVRYRIA
                     VDGANGKMGSFKLGLRLDPRPANDAFANATVLTGSDVTTEGTNFGATVEPGEPSNSLE
                     AASVWYSWTATTTGLVKVDLPRRTSSDLRPAVYTGDSVDKLTRVVYAGTPLHFRAEAG
                     TTYRIPVEGWSRAQGDFTLRVNEVPPPPNDHFANAIHLPGESGERRDTTSGATREAGE
                     WGPNDWERASVWYTWTAPADGLAVVRTKLTERSIYVRAYSGDSLSSLKLLTDTYGRSG
                     FRVRAGTTYRIAANASEGSEKGAFTLTWDFVRAPANDAFEDAIELTGMRAAARGRTTG
                     ATSEPEEKVNEWSGATVWYEWTAPSTGLTIVRSSRGTPTAYTGDSVATLTPLGSAYSS
                     EVRFRAVQGTTYRIRVANAINEPDGDFDLALEQFAPPPNDDFANAAPLTGSPARAVGI
                     MGGATSQPGEPDHGAGANPGTLWYSWTAPSSGTAVVTVPYAYPYNYAAAYTGDELGSL
                     RPRGTFQDRQLRFHARQGTTYRVAIAGELTGCCRDVPIELNLVSGPANDDFEDAAELV
                     GMTDVRTQGTANATREPREPNHDYYYNGSVWFRWTAPVSGRVTVDATGGETGAWIGAY
                     AGTSVDKLTLLRGASTKVSVPVRAGATYHFVAVGYRVDSAIRVGLAHIPAPANDDFAN
                     ATVLPSLAARGSGDSFASSREPAEPDHRSTTETNGTVWYRWTAPRSGSALVSLRGTKV
                     NAAFAAYSGSEIGSLTKLSSGYHAAAATSPTPLSFRATEGVTYSFVVEGWGHPGGPHE
                     IAVDMGTGPPNDDIAYATVLSGQTGSLDGNNSTATTESGEAPSNAGRSVWYRWTAPVS
                     KQVTFEAVGSGFDTQVAAYRGTQHGQLATMSSGNRQVTFEATAGTTYSIKVDGLYASS
                     AGGFFAFRWAQVEPPVNDNFAAAAPLRGSVAQMIGTTDGATAENQEGTHAGYGWLRSV
                     WFRWRAPGTGQVLITTDGSSFDTVLAAYGPAGSAAAARYNAVAANNDYGGGKQSAIAF
                     DATAGTIYNIAVDGVSGFSGRYRINLRFGATKPPNDDFASARELSGASVEDSGTTIGA
                     TPEAAEPAHAGARADASTWYRWTAPNDGTLRVSTAGSDFDTRLGVYTGEGLGSLSSIA
                     SDDDGAGNGASAAELEVAAGTTYRIAVDGADDGQGARQGRVKLTLGLTEPPPMPVPAP
                     LSAPDEGSEAASAPSSEGQPPAEEAPVSDASGDEPPAAGPSADPQPFEIEPPLEEEPP
                     PAAEGPAENTSAEEPPPAQPAEQPAAPATSANPPVEEPPAAEPPAQQSPVEETLKEEP
                     APGPAPDAAPGPAAPAPAPADEPAAAAPSLQPGDQSSGTDGSAPADTQPLEPTRPLEP
                     TRQEPPHQGPPVVDPADPGPRPDVTPQPPAAAPDPSPAPRLQLAATYPKQKLAAVLAK
                     GLAASFTCSIDCDLTVVVTVDAPTAKKLGLRGSRLTTIRATGRATAGRATKLVIKVPK
                     SLQAKLRKAR"
BASE COUNT         1511 a         3645 c         3433 g         1195 t
ORIGIN      
        1 catcaggaga gtcgcgccga cgcccatcac cgacatcgcc tgcgacccga acgcggccgg
       61 caccaccatg cccgcgaggt ggcggttcat cagcaggaag agccccggca gggtggccag
      121 ctggaagacg acggtcacgc cgtccttgaa gccgatcggc tccatgtgcc cgtcgggcag
      181 gaacggcagg cccatggtgc gcgacatgaa ccacaggccg agcgagctga ccgtgagcgc
      241 gaccgccccc cacagcagga gccgccgcgg gcgacccgcc agcaggaggc cgccgaccgc
      301 gatctggagc agcgcgacga tcaggaagcc gaccatcatc aacggcagcg cctcgtggtc
      361 ggaggcggcc gagaggtgga tgatcccggc gccgagcatg aagatcgccg tgacgtgcgc
      421 gatcagccga cctggatccc caaggttgga tccgtgtggc gccgtcaagc ggttgagcat
      481 gcccctgctt cctcccctgt taccgaccgt gctccggacc ccactcgcgg tctcgtatcc
      541 cccacccgcc cacggttaaa cccgccgtcg cgttcagcca gcattgagca cgtcacgggc
      601 tgcggatctc cttgacgagc agggcctgcg cgaagtagtt cagcccgaag cccgaagcga
      661 agctcgagct ggtcgcgccg agcatccgcg ccaggtccgt gcccgcgggc gtcctgacct
      721 cgccggtggc gtgcccgagc ttgaggtcgt tctcggcgta gagcttgacc tgcttgtcgc
      781 ccgacgggta ccagtaggtg atctggagtg ggatcgtgcc ctcgcgctcg atccccgacc
      841 cctcgagctc gaagggcgac ggcacgctcc cgcccacccg gaagcggaac ccgaactggc
      901 ccggaacgag ccccggccgc aggtcccatt cccagtcgcg cgtgacgtag acggggtagt
      961 cggaggttcc atcggcccga agccacctgg cccactcgcg gttgtcggtc gcgacgtaga
     1021 acgggtagaa ggtgcagtac ttgacgtagt cgccttggaa cgcctcgaat ccgaagtcgc
     1081 gctctccgtg ggacatgcag ccgctgtggt ccggcgactc gatcaccgcg ccgaagttgg
     1141 ccatgtaggt ggggatctcc ctgcccgaca ggtcgacccc cgcacacttg gagcccctga
     1201 ggtagaccgt cgcctggccg ggcgtctcct cgtagagcgt gtactgggcc gggacctgct
     1261 cgcgcacggg tcccttgggc acgttcacca gcgcgacgat ctcgtggcag tcgcgcaccg
     1321 tgccggaagt cgtctgggtg gtctgcgccg atgccgggct cgccgccagg cacgcgagta
     1381 ccgcgaccgc cgccgctacc gccgcccgcg cgaacgatcc atgtcgcagc gcccccccaa
     1441 cgaccatttc tacctccgcg atttccggtt gagcatgccc ctacttcttc ccccgcgtgc
     1501 ccggctgcgc gtagacgccg aggcccccct ggcgccctgc gcgtggcgcc ggacccccgg
     1561 gggctctcgt accccgggct aggtcactta aacctggact tcgcgctcag cgcgggcgcc
     1621 gtacggccgc gtccgcgacc gccgtaccgc cctcgtcgat ctcgatcccg gccgtctcgg
     1681 ccacgcgcgc gaccagcatg tactggccga tcacgagcag cagctcgacg acctcgcgag
     1741 gggagagcga gcggcgcacc tcggcgagcg tctcgtccga cgcgcgcacc tcgcgcacga
     1801 cctcctcggt gaagcgcagc accgcccggt cgctctcgcc gaaggccgac aggtcgccgc
     1861 gctcgacggc cgcgacctcg tcccgcgtcg cgccgaccgc cagcgcgatc ggcacgtgtt
     1921 gggcccactc gtactccgcc cccgacaggc gaccgacgtg caggatcacg agctcgcgca
     1981 cgcgcgcgtc gagctccagg gacgacagca gggcgccccc gaacgcgagc cacggcctga
     2041 acgcggtctc ggcgttcgcg accatcctga agatgttgat cggcggcacc cgctcgagcg
     2101 cctcgcgcac cggatcggag gcgtcctcgg ggtccacgta gggaaggcgt gccacggcac
     2161 gagcttacgg cgcgtcaccc cgtctgcgtc gcggcggcag gtgggggtta cgcgccaccg
     2221 acccgagggg tatgctgccg ctcgtacccc gggggaacgc ccatgcgcca ggacacatag
     2281 cggtgcggcg cgctctggcc ttttgcgcgc ttgtcttgct cgcggccgca ttcgcggcct
     2341 cgtcggcaca cgcggcaacg cccccgccga acgacaactt cgccagcgcc gagctgctga
     2401 gcggccgctc cgcgtcggcc ggcgggacga ccgacggcgc cacgtccgag cccggcgagc
     2461 cctacgacta ctggtacggc aagacgatct ggtaccgctg ggtcgccccc gccgacggca
     2521 ccgtcgccgc tgacgtcggg gccagcgcga taccggcggt cgtcacggcg ttcacgggca
     2581 gttccgtcgg cgcgctgacc cgcgtgccgt ctcccgagac gaacggcagc acgtcgaagc
     2641 gggcgcggtt caccgtctcc gccgggacga cgtaccactt gcgagtcgaa tccggctcgt
     2701 acgaccggaa cggcccggtc gcgatcgccc tcgagctgcg cgatccgccg ccgaacgaca
     2761 ggttcgcctc gccccaggtg ctgaccggcg acgaggccac ggccaccggt gacacgagct
     2821 gggcgacccg cgagaccggc gagccgcgcg cggcgagcgg gtccggcggc ggaaccgtct
     2881 ggtactcgtg gaccgcgccc tcgaccggcg gggtcacgct gagcaccgag ggcagcgcga
     2941 tcgacacggt gctcggcgtg tacacgggcg ccgcgctcag ctcgctcgtg caggtaagcg
     3001 cgaacaacga cgcgcgctcg ggagtccgca cgagccgcgt gacgttccgc gcagaggcgg
     3061 ggaccaccta ccgcttcgtc gtcgacggct actcgagcgg ccaggtcggc gcggtgcggc
     3121 tggcgctcag cctccgcccg ccaccggcca acgacgcgtt cgcgggcgcc accgtcctcg
     3181 acagtgcccg cgacctgacg gtgatcggca acaacaacgg cgcgagcgcg gagctgggcg
     3241 agccgcagca ctactcctac tacgcgccga agagcagcgt gtggttccgc tggaccgcgc
     3301 cgagcaccgg ctcgctcacg atccgcgcga cgaccgactt cgcaccggtg ctggcggcgt
     3361 acacgggcaa ctcggtcgcc gcactcacgc gcgtgccgaa ccagccgcag cagtggaacg
     3421 gcgggccgga gcagatccgc ctgcgcgtga acgccgggga gacgtactac atcgcgctcg
     3481 acagctacgt cgtacacggc gatttctcgc tgaacctcgc gctgatcgac aaccccggca
     3541 acgacgacct cgcggacgcc gtcgagctgg agggcgcatt cgtcgaggtg acgagcgaca
     3601 acgtcggcgc cacccaggag ccgtgcgagc cgcgccacga cgacaactac tacgacccgt
     3661 cggtctggta cgagtggacg gcgcccgccg acggcggcgt ggtcgtggac acctcgggca
     3721 gcgacctgct caccgcagtc gggatctaca cgggcgactc gctctgctcg ctcactcgcc
     3781 tgcccgtgac gcgtctgtcg gccacaggga ccccggcggt gcgcacgttc accgccaagg
     3841 gcggcaccac gtaccggatc gcgatcgacg gccaggccgc gcggatgggg cgcttccggc
     3901 tctcccttcg gctcacgccg ccgccggaca acgacatgtt cgccggcgcg cagccgctgg
     3961 ccggcccctc ggcgagcgcg gcggggaaca cgctcggcgc ctccgccgag gccggcgagc
     4021 ccgcggcgaa cgggccctcg agcgtgtggt tcgaatggac ggcgccgtcg agcgggcgga
     4081 ccaccctcgc ggcgctcagg gccaccggcg agctggcggt ctacaccggc gacgccgtcg
     4141 gctcgctctc cgccgtctac acgcgcggat accgcagcca gctcgtcttc cacgcgcgcg
     4201 cgggagtgac gtaccggatc gcgctccacg gcggcgccgg agccgcgcag ggcccgtacg
     4261 agctcggcct gacgcacgtg accgggccgg cgaacgacga cttcgcggat gcgacgccgc
     4321 tccccgccac cgacgcgacg gtggtcggct ccaccgccgg cgcgacgcgc gagctgaacg
     4381 agccgcgcct cgactacgac tacgcggagc gctcgatctg gtactcgtgg accgccacgg
     4441 cggacgccaa cgtcgtgctc gacacctacg ggagcggctt ccgcaccggc atgggcgtct
     4501 acaccgggga gtcggccgcc gcgctcgtgc gggtcgccgg gcactacggg tccgacaagc
     4561 ccgcgcgcgt cggcttccgc gccacggccg ggacgaccta ccggataaag atcgacggcg
     4621 cctacgaggc cgacttcggc gacgtcaagc tcgcgctggc cgtgcgcggg aaccccccga
     4681 acgacaacat cgcggacgcg aagtcgctgc cctcggctcc cgcggtgtcg atcgcgggct
     4741 ggaacgccgg cgcgaccgcg gagaccggcg agccgaccca caactcgtac tggtccgcga
     4801 accagtcggt ctggtaccgg tggatcgctc cgcggaccgg gtccctcacg atccgctccg
     4861 acgcccactt ccagacggtg atcgccgcct acacgggcag cgtgatcggc agcctcagcc
     4921 gggtcgccaa ccagccgcag gactggaacg gcggcaagga gcagatccgc atccgggtcg
     4981 aggccggcaa gcagtacatg atcgccgtcg acggcgacac gagcggctac cgcggctacg
     5041 gcgacttcca gctctcgctc cagctcgcgc agggcccgcc gaacgacgag ttcagcagcc
     5101 ccctgccgct gtccggcgcg ctcaccgagc tcgtcggcag caccgtccac gcgacgcagg
     5161 agccctgcga gccggtccac gacggcaact actacgaccc gtcggtctgg tacgagtgga
     5221 ccgcgcccga gaccggcccg gtcacgatcg acaccaccgg cagcgacttc cagaccgtcg
     5281 tcgcggtcta caccggcgac gtgctgtgca cgctggcgcg cgtcgagacg ctggccttca
     5341 gcacgggcac gcagccggcg aagcgcgggt tccgcgcgaa ggcgggagtg aggtaccgga
     5401 tcgccgtaga cggcgcgaac ggcaagatgg gcagcttcaa gctcgggctg aggctcgatc
     5461 cgcggcccgc caacgacgcc ttcgcgaacg ccacggtgct caccgggagc gacgtcacga
     5521 ccgaggggac caacttcggc gccacggtgg aaccgggcga gccgagcaac agcctcgagg
     5581 ccgcgtcggt ctggtactcg tggacggcga cgacgacggg gctcgtgaag gtcgacctcc
     5641 cgcgcaggac gtcgtccgac ctgcgtcccg ccgtctacac gggcgactcc gtcgacaagc
     5701 tcacgcgcgt cgtatacgcc ggcacgccgc tgcacttccg tgccgaggcg ggcacgacct
     5761 accgcatccc ggtggagggc tggtcgcgcg cgcagggcga cttcacgttg cgcgtgaacg
     5821 aggtcccccc gccgccgaac gatcacttcg cgaacgcgat ccacctgccg ggcgagtcgg
     5881 gcgagcggcg cgacactacc tcgggcgcga cccgcgaggc cggcgagtgg ggtccgaacg
     5941 actgggagcg cgcgtcggtc tggtacacgt ggaccgcgcc cgcggacggc ctcgcggtgg
     6001 tcaggacgaa gctcaccgag cgcagcatct acgtccgcgc ctacagcggc gactcgctca
     6061 gctcgctgaa gctgctcacc gacacctacg gccgctccgg gttccgcgtg agggcgggca
     6121 cgacctaccg catcgcggcc aacgcgagcg agggctccga gaagggcgcg ttcaccctga
     6181 cctgggactt cgtccgcgcg cccgccaacg atgcgttcga ggacgcgatc gagctgaccg
     6241 ggatgcgtgc ggccgcgcgc ggcaggacca ccggggcgac cagcgagccc gaggagaagg
     6301 tgaacgagtg gtcgggcgcg accgtctggt acgagtggac cgcgccgtcc acgggcctga
     6361 cgatcgtccg gtcgagccgt ggcacgccga ccgcctacac cggcgactcg gtggccacgc
     6421 tcacgccgct cgggagtgcg tactcgagcg aggtgcggtt ccgtgcggtg cagggcacca
     6481 cctaccggat ccgcgtcgcg aacgcgatca acgagccgga cggggacttc gacctcgcgc
     6541 tcgagcagtt cgctccgccc ccgaacgacg acttcgccaa cgcggccccg ctgacgggga
     6601 gcccggcgcg ggcggtcggg atcatggggg gagcgacgag ccagcccggc gagccggacc
     6661 acggcgcggg ggccaatccc ggcacgctct ggtactcgtg gacggcgccg tcgagcggca
     6721 ccgcagtcgt cacggtgccg tacgcgtacc cgtacaacta tgcggcggcg tacacgggcg
     6781 acgagctcgg gtcgctgcgt ccgcgcggca cgttccagga ccgccagctg cgcttccacg
     6841 caaggcaggg caccacctac cgggtcgcga tcgccggtga gctgaccggt tgctgccgcg
     6901 acgtgccgat cgagctcaac ctggtcagcg gacccgcgaa cgacgacttc gaggacgcgg
     6961 ccgagctcgt tggcatgacc gacgtgcgga cgcagggcac ggccaacgcg acgcgcgagc
     7021 cgcgcgagcc gaaccacgac tactactaca acggcagcgt ctggttccgc tggaccgcgc
     7081 cggtcagcgg gcgggtcacc gtcgacgcca ccggcggtga gacgggggcg tggatcgggg
     7141 cgtacgcggg cacgagcgtc gacaagctca cgctgctgcg cggcgcgtcg acgaaggtca
     7201 gcgtgccggt gcgcgccggc gcgacctacc acttcgtcgc ggtcgggtac cgggtggact
     7261 ccgcgatacg ggtcgggctg gcgcacattc ccgcccccgc gaacgacgac ttcgcgaacg
     7321 cgaccgtgct gccgtcgctc gcggcgcgcg gcagcggcga cagcttcgcc tccagtcgcg
     7381 agccggcgga gccggaccac cgctcgacca ccgagacgaa cggcacggtc tggtaccgct
     7441 ggacggctcc gcgcagcggc tccgcgctcg tgagcctgcg cgggacgaag gtcaacgctg
     7501 ccttcgcggc ctactcgggc tcggagatcg gctcgctgac gaagctcagc tcgggctatc
     7561 acgcggcagc cgccacgtcc ccgacgccgc tgtcgttccg cgcaaccgag ggagtcacgt
     7621 acagcttcgt ggtcgagggg tggggtcatc ccggcgggcc gcacgagatc gcggtcgaca
     7681 tgggcacggg gccgccgaac gacgacatcg cgtacgcgac cgtgctgagc gggcagaccg
     7741 gcagcctcga cggaaacaac tcgaccgcga cgaccgagtc gggcgaggca ccgtcgaacg
     7801 ccggcaggtc cgtctggtac cgctggaccg cgcccgtgag caagcaggtc accttcgagg
     7861 cggtcgggtc cggcttcgac acgcaggtcg ccgcgtaccg ggggacacag cacgggcagc
     7921 tcgcgacgat gtcctcgggc aatcggcagg tcaccttcga ggcgacggcg ggcaccacgt
     7981 actcgatcaa ggtcgacggc ctgtacgcga gcagcgccgg cgggttcttc gcgttccgct
     8041 gggcgcaggt ggagccgccg gtgaacgaca acttcgccgc ggccgcgccg ctcaggggct
     8101 cggtggcgca gatgatcggg acgaccgacg gagcgacggc ggagaaccag gaggggacgc
     8161 atgccggtta cggctggctc aggtcggtct ggttccgctg gcgtgcgccc ggtacgggcc
     8221 aggtcctcat cacgaccgac ggcagctcgt tcgacaccgt gctcgcggcc tacggaccgg
     8281 cggggtccgc cgccgccgcg cgttacaacg cggtcgccgc gaacaacgac tacggcggtg
     8341 gcaagcagag cgccatcgcg ttcgacgcga cggcggggac gatctacaac atcgccgtgg
     8401 acggagtaag cgggttctcc ggccgctaca ggatcaacct gcggttcggt gccacgaagc
     8461 cgccgaacga cgacttcgcg agtgcgcgcg agctgagcgg ggcctcggtc gaggactcgg
     8521 gtactacgat cggcgcgacc cccgaggccg ctgagccggc acacgcgggc gctcgcgccg
     8581 acgcgtcgac gtggtatcgc tggacggcgc cgaacgacgg gacccttcgc gtgtccacgg
     8641 ccgggagcga cttcgacacc cgactcggcg tctacacggg tgaggggctc ggctcgctgt
     8701 cgtcgatcgc gtcggacgac gacggggcgg gcaacggcgc gagcgcggcc gagctcgagg
     8761 ttgccgccgg gaccacgtac cggatcgcgg tggatggcgc ggacgacggg cagggcgccc
     8821 ggcagggtcg cgtgaagctg acgctcggtc tgaccgagcc gccgccgatg ccggtgccgg
     8881 cgccgctgtc cgcgccggac gagggcagcg aagcggcctc cgcgcccagc tcggaggggc
     8941 agccgcccgc cgaggaggcg ccggtgagcg acgcgtccgg agacgagccc ccggcagcgg
     9001 gaccatccgc cgacccgcaa cccttcgaga tcgagccgcc gctcgaggaa gagccgccgc
     9061 cggccgcgga agggccggcc gagaacacct ccgccgagga gcctccgccg gcgcaacccg
     9121 ccgagcagcc ggctgccccc gcgacgagcg ccaacccgcc ggtcgaggag ccgccggccg
     9181 cggagccgcc cgcccagcag tctccggtcg aggagaccct caaggaggag ccggctcccg
     9241 ggccggcgcc cgacgcggct cccgggccgg cagcacccgc cccggctccg gccgacgagc
     9301 ccgccgccgc cgcgccgtcg ctgcagcccg gcgaccagtc ctccggaacg gatgggtcgg
     9361 cgcccgccga cacgcagccg ctggagccca cgcggccgct ggagcccaca cgccaagagc
     9421 cgccgcacca gggtccgccc gtcgtcgatc cggcggaccc cggcccgcgc ccggacgtga
     9481 cgccgcagcc accggccgcc gctcccgatc cttcccccgc cccccgcctc cagctcgccg
     9541 cgacctaccc gaagcagaag ctcgccgccg tgctcgccaa gggactcgcc gcaagcttca
     9601 cgtgctcgat cgactgcgac ctcacggtcg tcgtgaccgt cgacgcaccc accgcgaaga
     9661 agctcgggct gcgcggcagc agactcacca cgatcagggc gaccggccgc gccaccgccg
     9721 ggcgcgccac gaagctcgtg atcaaggtgc ccaagtccct ccaggcgaag ctcaggaagg
     9781 cgcg
//
LOCUS       DASVPL010000064         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_321132, whole genome shotgun sequence.
ACCESSION   DASVPL010000064 DASVPL010000000
VERSION     DASVPL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321132"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VF517_03180"
     CDS             <1..336
                     /locus_tag="VF517_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051967.1"
                     /translation="QSRLSGLVKGTFRGIDVVQTGSSPRIVYADVVGSSGRTRVTGPT
                     LRARLGLYDTWFTVKKVTGTEAKRYKPTPTKRTQRGTPSSLQSSGWVQSSPATSPGSS
                     PAESWQWLG"
     gene            267..1232
                     /locus_tag="VF517_03185"
     CDS             267..1232
                     /locus_tag="VF517_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8051968.1"
                     /translation="MGAIVARDLAREFSGGVVAVAGIDLDVAEGEIYAFLGPNGAGKT
                     TSVRMLTTLLRPTGGSATVAGHDVVADASAVRRNIGVALQEAALDPLMTGREMMQLQS
                     ALHAVPRREAKPRSDALIGRVGLDYAADRRVGTYSGGMRRRLDLAMALVHEPRVLFLD
                     EPTTGLDPASRSAIWNEVRALNDEGTTVFLTTQYLEEADQLADRVGIIDQGGMVAEDT
                     PENLKAEVGRPSLEVSVSGDRARATEVLGRFGPVRGQKDSLAVPLEDHSGVTDVVRAL
                     DEAGLMVDSLEVRRPTLDDVFLVKTGHALDPVDAAADEDEAAAPA"
     gene            1229..2008
                     /locus_tag="VF517_03190"
     CDS             1229..2008
                     /locus_tag="VF517_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8051969.1"
                     /translation="MIAADLRATGALARRSLSQTFRYPRYLAPLVVFPSLFLAVNVGA
                     AGRSTALPGFPDVHGFLDFELAAAMLQASMLTGVSSGMALGIDIERGFIDRLIAAPVS
                     RPVVVTGRLAANAALGVFIGTWFLTIGLVFGAHVAGGVIGVLQVMLLLALAALAFGAL
                     GAALALWAGNAGLVQGTFPLVFVVLFVSTAFFPRALMQEPAASIAAWNPLSLIAHGLR
                     EPVIDGVTLASFAEGLAGVGIIGAISSGMAALALRHRLRGA"
     gene            2009..2782
                     /locus_tag="VF517_03195"
     CDS             2009..2782
                     /locus_tag="VF517_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8051970.1"
                     /translation="MSEWRADLAVASALVRRNFNEILRVPGGAVPGIIAPTIFLVGLT
                     SVFGEIQRLPGFTADEFISFLIPITLLQAAAFSGAATGVNLARDIELGWFDRILVSRA
                     PRPVILAGLLAAASLRSLLPIAVALVVAFSLGAAFPGAAGLLLAILIACVFAGIAAAW
                     AMIIAMRFRTQSAAPLMQATGFMAVLFTTSYAPEDLLSGWFGDIAHYNPMTKVVEAVR
                     QGFVGGLSWADTWPGLVVISALAALLVAFALRGLLRMGH"
     gene            2920..3105
                     /locus_tag="VF517_03200"
     CDS             2920..3105
                     /locus_tag="VF517_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051971.1"
                     /translation="MRCMQCAAVSFSAAARTMVEQGYRCGQCGGELVLVPVEAPVAVA
                     VAGDADDGAHERRFERE"
BASE COUNT          400 a         1145 c         1124 g          480 t
ORIGIN      
        1 cagtcgcgcc tgtccggcct cgtgaaggga accttccgag gaatcgacgt cgtccagacg
       61 ggcagctcgc cgcgcatcgt ctacgccgac gtggtcggct cgagcggccg cacgcgcgtg
      121 accggcccga cgctgcgtgc ccggctcggc ctctacgaca cctggttcac ggtgaagaag
      181 gtgaccggca ccgaggccaa gcgctacaag cccacgccga cgaaacgcac acagcgcggc
      241 acccccagct cattacagtc atccggatgg gtgcaatcgt cgcccgcgac ctcgccaggg
      301 agttctccgg cggagtcgtg gcagtggctg ggatagacct cgacgtagcc gagggcgaga
      361 tctacgcgtt cctggggccg aacggcgccg ggaagacgac cagcgtgcga atgctcacca
      421 ccctgctgcg cccgaccggc ggcagcgcga cagtggccgg ccacgacgtg gtcgcggacg
      481 cgtcagcggt gcgacgcaac atcggcgtcg cgctccagga ggccgcactc gacccgctca
      541 tgacgggccg cgagatgatg cagctccagt ccgcgctcca cgcggtgccg cgccgcgagg
      601 ccaagccgcg ctcggacgcc ctgatcggac gcgtcgggct cgactacgcc gcggaccgcc
      661 gcgtcggcac ctactccggc gggatgcgcc gccggctcga cctcgcgatg gccctcgtgc
      721 acgagccgcg cgtgctgttc ctcgacgagc cgaccaccgg cctcgacccc gccagccgct
      781 cggcgatctg gaacgaggtg cgcgcgctca acgacgaggg cacgacggtc ttcctcacca
      841 cccagtacct ggaggaggcc gaccagctcg ccgaccgcgt cgggatcatc gaccagggcg
      901 ggatggtggc cgaggacacg ccggagaacc tgaaggccga ggtcgggcgg cccagcctcg
      961 aggtgagcgt gagcggcgac cgcgcccgcg ccacggaggt gctgggacgc ttcggcccgg
     1021 tccgcgggca gaaggacagc ctcgccgtgc cgctcgagga ccactcggga gtgaccgacg
     1081 tggtgcgcgc tctcgacgag gccgggctga tggtcgactc gctcgaggtc cgccggccca
     1141 cgctggacga cgtgttcctc gtgaagaccg gccacgcgct cgacccggtc gacgctgccg
     1201 cggacgagga cgaggccgcc gcgcccgcgt gatcgccgcc gacctccgcg ccaccggcgc
     1261 gctcgcccgc cgctcgctca gccagacctt ccgctacccg cgctacctcg cgccgctggt
     1321 cgtcttcccc tcgctgttcc tcgcggtgaa cgtgggggcc gctgggcgct ccaccgccct
     1381 gcccggcttc cccgacgtgc acggcttcct cgacttcgag ctggccgccg cgatgctgca
     1441 ggcctcgatg ctcacgggcg tcagctcggg gatggcgctc gggatcgaca tcgagcgcgg
     1501 cttcatcgac cgcctgatcg cggctccggt ctcgcgcccg gtcgtggtga ccgggaggct
     1561 ggcggccaac gccgcgctcg gggtcttcat cggcacctgg ttcctgacga tcggcctggt
     1621 gttcggcgcg cacgtcgcgg gcggggtgat cggcgtgctc caggtgatgc tgctgctcgc
     1681 gctggcggcg ctcgcgttcg gcgcgctcgg cgcggcgctc gccctctggg ccggcaacgc
     1741 cgggctcgtg caggggacgt tcccgctcgt gttcgtggtg ctgttcgtct cgaccgcgtt
     1801 cttcccccgt gcgctgatgc aggagccggc ggcgtcgatc gcggcgtgga accccctgag
     1861 cctgatcgcg cacgggttgc gcgagcccgt gatcgacggc gtcacgctgg catcgttcgc
     1921 ggaggggctc gccggggtgg ggatcatcgg cgcgatcagc tccggcatgg ccgccctggc
     1981 cctgcgccac cggctgaggg gcgcttaggt gagcgagtgg cgcgccgacc tggccgtggc
     2041 gagcgccctc gtgcggcgga acttcaacga gatcctgcgc gtgcccggcg gagcggtgcc
     2101 cgggatcatc gcgccgacga tcttcctggt cggcctcacg agcgtgttcg gcgagatcca
     2161 gcggttgccc ggcttcactg ccgacgagtt catctcgttc ctcatcccga tcacgctgct
     2221 ccaggcggcc gcgttctccg gagccgcgac cggcgtcaac ctcgcgcgcg acatcgagct
     2281 cggctggttc gaccgcatcc tcgtctcgcg cgccccccgc cccgtgatcc tcgccgggct
     2341 gctggcggcg gcgagcctgc gctcgctgct cccgatcgcc gtcgcgctcg tggtggcgtt
     2401 ctcgctcggc gcggcgttcc cgggcgccgc cgggctgctc ctggccatcc tgatcgcgtg
     2461 cgtgttcgcg ggcatcgcgg ccgcgtgggc gatgatcatc gccatgcgct tccgcacgca
     2521 gtcggcggcg ccgctcatgc aggccaccgg cttcatggcg gtcctgttca cgacctccta
     2581 cgcccccgag gacctcctca gcggctggtt cggcgacata gcgcactaca acccgatgac
     2641 gaaggtcgtg gaggccgtcc ggcagggctt cgtcggcgga ctctcgtggg ccgacacgtg
     2701 gcccggactg gtcgtgatct ccgcgctggc ggcgctgctc gtggccttcg cgctgcgcgg
     2761 cctgctgcgc atgggccact gatctctggc gcggttcggc gccaggcaaa ttcgattggt
     2821 ggcataaaac ccccagttcg gggataatca caaggagccg ggcgttcggc aggggaaagt
     2881 tcggacgccc cgcgagggga agaacaaggc ttggaccaga tgaggtgcat gcagtgcgcc
     2941 gccgtgtcgt tctcggcggc ggctcgaacc atggtcgagc agggctaccg ctgcgggcag
     3001 tgcggcgggg agctggtgct cgtccccgtc gaggcccccg tggccgtcgc cgtggccggc
     3061 gacgcggacg acggcgcgca cgagcgccgc ttcgagcgcg agtagccgcc cgcccgcccg
     3121 ctagggcgtg aacttgatgg tgatcacgt
//
LOCUS       DASVPL010000065         3275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_361436, whole genome shotgun sequence.
ACCESSION   DASVPL010000065 DASVPL010000000
VERSION     DASVPL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361436"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VF517_03205"
     CDS             <1..439
                     /locus_tag="VF517_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051972.1"
                     /translation="DGYKPFPIVEGRWIHMTNQERYADGTLIDPDKIDSFMFARVGND
                     WIPVGGMYMYGNRADPNPPNPTGCLLMWHRHASYEGYAATAAYTWDDVTISEKTMWMA
                     HVWTYGNLDPWGRDFDGTEPASWSWPSSMLPNGCDRKNFCPTP"
     gene            546..1790
                     /locus_tag="VF517_03210"
     CDS             546..1790
                     /locus_tag="VF517_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX8051973.1"
                     /translation="MDHFDDLAGALRRRMIEQARDGAAAGGDLAADARALVEREAAAL
                     PPAEREALVARVIMLATGLGPLDPLLADPAVDEVMVNGPGEVWVERGGRVEPTGVAFG
                     DEAELMHAIERILAPLGRRVDEASPLCDARLADGSRVNVVIPPLALSGACLTIRRFRP
                     AGFSLDDLVDNGTLPRGLAEFLGSCVRARAAVLVSGGTGSGKTTTLGALSGAVPDGER
                     IVTIEDAAELRLRQRHVVRLESRPANVEGRGEVTIRQLVRNALRMRPDRIVVGEVRGA
                     EALDMLMALNTGHEGSLTTVHANSPEDALRRVETLALMAGLGLPHAAVREQVASAIDL
                     VVHQTRCADGRRRIECVAEVVRAAGGAGTRELYRLRGDRPLWRPPAAGRLAERLGRAG
                     GGPASRPPDGAASPDGAASRAP"
     gene            1787..2653
                     /locus_tag="VF517_03215"
     CDS             1787..2653
                     /locus_tag="VF517_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8051974.1"
                     /translation="MSTAAALAFAAGALGLLAAVDLAREVGDAAGRRAPGFARAAGGL
                     VESVLRAGREGRDPGAAERRRLLVTAGAVAFAIGRFVAGPAIGVAAAVAGPVAIARAL
                     RAGRERYRAAAGAGAAALAAALADALAGGHSVRGAIALAAPAIEGPAGAELRRVASEL
                     ELGARTDDALEALRARVRSHGIDAIVAALLLQRASGGDLARLLRDCARSLDEEARVEA
                     DARAATAQARFTGVIVVLLPLGGALLAELGSPGFAARMAGSFLTAWLAGLAVGLQVVA
                     AILIRRLGRVRA"
     gene            2694..>3275
                     /locus_tag="VF517_03220"
     CDS             2694..>3275
                     /locus_tag="VF517_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051975.1"
                     /translation="MTASAALAGLAAAAGAVGLAELSAGLRPRGTRRSPGDGPALRRV
                     LARAGARLRAIAGARAPVTLEARIAAAGAPAGLGPRELMAAKLAGALASAPVAALLGA
                     AAPGRLGPLLVVAAPVGGFLAPDWWLARRARERARVVRRDLPALLDLLRVSVEAGLAP
                     PAALRAVAERGSGPLAAEWATVAREVALGVPLAT"
BASE COUNT          331 a         1209 c         1335 g          400 t
ORIGIN      
        1 agacggctac aagccgttcc cgatcgtcga aggccgctgg atccacatga ccaaccagga
       61 gcgctacgcc gacggcacgc tcatcgaccc cgacaagatc gactcgttca tgttcgcgag
      121 ggtcgggaac gactggatcc ccgtcggggg catgtacatg tacggcaacc gggcggaccc
      181 gaacccgccg aacccgacgg gctgcctgct gatgtggcat cgccacgcga gctacgaggg
      241 ctatgcggcg acggcggcgt acacctggga cgacgtcacc atctcggaga agaccatgtg
      301 gatggcgcac gtgtggacct acgggaacct cgacccctgg ggccgcgact tcgacgggac
      361 cgagccggcg agctggtcgt ggccgtcgtc gatgctcccg aacggctgcg accgcaagaa
      421 cttctgcccg accccctgac gcgctagccg gggcgaggat cgcgcggcac cccgccagcc
      481 ggggcgaatg gcgtgccgcg gccgctggcc ggggcgaaaa tcgtgcggca cgcccttaac
      541 ggggcgtgga ccacttcgac gacctcgccg gcgcgctgcg ccggcgcatg atcgagcagg
      601 cacgcgacgg cgctgccgcg ggcggcgacc tcgcggccga cgcccgggcg ctggttgagc
      661 gcgaggcggc cgcgcttccg ccggccgaac gcgaggcgct ggtggcgcgc gtgatcatgc
      721 tcgccaccgg cctcggtccg ctcgacccgc tgctcgccga cccggccgtg gacgaggtga
      781 tggtgaacgg gccgggggag gtgtgggtcg agcgcggcgg gcgcgtggag ccgaccggcg
      841 ttgcgttcgg ggacgaggcg gagctgatgc acgcgatcga gcggatcctg gctccgcttg
      901 gccggcgggt ggacgaggcg tcgccgctgt gcgacgcgcg gctggccgac gggtcgcgcg
      961 tgaacgtggt gatcccgccg ctcgcgctct cgggcgcgtg cctgacgatc cggcgcttcc
     1021 gcccggctgg cttctcgctc gacgatctcg tcgacaacgg aacgctgccc cgcgggctcg
     1081 cggagttcct cggttcgtgc gtgcgcgcgc gggccgcggt gctcgtgagc ggcggcaccg
     1141 ggtcggggaa gacgacgacg ctgggtgcgc tgtcgggtgc cgtcccggac ggggagcgga
     1201 tcgtgactat cgaggacgcg gccgagctgc ggctgcgcca gcggcacgtg gtccggctcg
     1261 agtcgcggcc ggcgaacgtg gaggggaggg gggaggtgac gatccgccag ctcgtacgca
     1321 acgccctgag gatgcgcccg gaccggatcg tggtgggcga ggtgcgcggg gcggaggcgc
     1381 tcgacatgct gatggcgctg aacaccgggc acgagggatc gctcacgacc gtgcacgcga
     1441 actcgcccga ggacgcgctg aggcgggtgg agacgctcgc gctgatggct gggctcgggc
     1501 taccgcacgc ggcggtgcgc gagcaggtgg cgagcgcgat cgacctggtg gtgcaccaga
     1561 cgcgctgcgc ggacggccgc cggcggatcg agtgcgtggc ggaggtggtg cgcgcagcgg
     1621 gcggagccgg cacgcgcgag ctctaccggc tgcgcggcga ccggccgctc tggcgcccgc
     1681 cggccgcggg ccggctggcg gagcggctgg ggcgggcggg cggggggccg gcgtcgcgcc
     1741 cgccggacgg agcggcttcg ccggacggag cggcttcgcg cgcgccgtga gcaccgccgc
     1801 cgcgctcgcc ttcgccgccg gcgctctcgg tcttctcgcc gccgtcgatc tcgcgcgcga
     1861 ggtgggcgat gcagccgggc ggcgggcgcc cggcttcgcg cgggccgccg gcgggctcgt
     1921 cgagagcgtc ctgcgcgctg ggcgcgaggg gcgcgacccg ggcgccgccg agcgccggcg
     1981 gctgctcgtg accgcggggg cggtcgcctt cgcgatcggg cggttcgtcg ccgggcccgc
     2041 gatcggcgtg gccgccgcgg tcgcggggcc ggtcgccatc gcccgcgcgc tgcgcgctgg
     2101 ccgcgagcgc taccgcgcgg ccgccggagc gggtgccgcc gccctcgccg cggcgctcgc
     2161 ggatgccctc gcgggcggcc actccgtgcg gggtgcgatc gcgctcgccg ctccggcgat
     2221 cgagggcccg gccggtgccg agctgcggcg cgtggcgagc gagctcgagc tgggcgcccg
     2281 gaccgacgac gcgctcgagg cgttgcgtgc ccgggtgcgc tcgcacggga tcgacgccat
     2341 cgtcgcggcc ctgctgctcc agcgcgcctc gggcggagac ctcgcccggc tcctgcgcga
     2401 ctgcgcccgc tcgctcgacg aggaggcccg cgtggaggcc gacgcccgcg ccgccaccgc
     2461 gcaggcgcgc ttcacggggg tgatcgtggt gctgctcccg ctgggaggcg cgctgctggc
     2521 cgagctgggc agcccaggct tcgccgcgcg catggccggg tcgttcctca cggcgtggct
     2581 cgctggactc gccgtcgggc tccaggtcgt ggcggcgatc ctgattcgcc ggctggggag
     2641 ggtgcgggcg tgaagccggc gccgcgctcc cgcggccagg ccggggaccc gcggtgacgg
     2701 cctccgccgc cctcgccggc ctcgccgcgg ccgcaggcgc cgtcggcctc gcggagctct
     2761 ccgctggcct gcgcccgcgg ggcacgcgcc gatcccccgg cgacggaccg gccctccggc
     2821 gcgtgctcgc cagagcgggc gctcgccttc gcgccatcgc cggcgcccgc gcaccggtca
     2881 cgctcgaggc gcggatcgcg gccgcgggcg cacccgccgg gctgggcccg cgcgagctga
     2941 tggccgcgaa gctggccgga gcgctcgcct cggcaccggt cgccgcgctc ctcggcgccg
     3001 ccgcgcccgg gcggctcggg ccgctgctcg tcgtcgcggc gccggtcggc ggcttcctcg
     3061 cccccgactg gtggctcgcg cgtcgcgcgc gcgagcgcgc gcgagtggtc cgacgcgacc
     3121 tgcccgccct cctcgacctc ctccgcgtgt cggtcgaggc ggggctcgcg cccccggccg
     3181 cgctgcgggc ggtcgccgag cgcggctccg gtccgctcgc ggcggagtgg gccaccgtgg
     3241 cgcgcgaggt cgcgctcggc gtgccgctcg ccacc
//
LOCUS       DASVPL010000066         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_361581, whole genome shotgun sequence.
ACCESSION   DASVPL010000066 DASVPL010000000
VERSION     DASVPL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361581"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="VF517_03225"
     CDS             <1..1783
                     /locus_tag="VF517_03225"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl reductase"
                     /protein_id="HEX8051976.1"
                     /translation="GARADGTWLVLRDRGEVATELVAAIEARGGRALLVDDDIASPEV
                     ARTLIASAESPLRGVVHLRGLDSPEMPAGGLAELRDATRRDCGALLHVVQALAAAEKP
                     QPQLWLATRGVQPAGGAAPAPLHAPLWGLGRVVALEHSELWGGMVDLDPAGMPGEAAL
                     LLDEILASDGEDQVAHRRGERYAARLVPGALDAGRGVLLRPDASYLVAGGRGALGLKL
                     AAWMADRGARHLILTGRGPVTREAAAAVARLEERGVSVDCPRADVADPDAMAAVLDAV
                     EPPVRGVVHAAGVFEPRRAADMSRAEFEEVAAAKIEGTWVLHELTRATELDFFVMFSS
                     AASVWGSALAGHYVAGNHFQDVAAHHRRGLGLPALAVNWGWWEGSDMVPEEAKAYFGA
                     LGLEVVPEDAGFDALERLLESKAVQRTIAPVDWRTFKAAFSARRRRPLLDLIEVPVEP
                     GGAASAEGLELIRALEQAPARRRGELIVDFLQRLVGAALGLDRPMDPQLGFFDAGMDS
                     ITAVEVKTRIESSLGVAIPATAAFEHPNIEALATYLLGDVLELAAPEPVEEKPPEANG
                     NGHAELDGLSEDELLDLLENQLAGAER"
     gene            1780..>2020
                     /locus_tag="VF517_03230"
     CDS             1780..>2020
                     /locus_tag="VF517_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8051977.1"
                     /translation="MSAPAERREEIIRRALVELGEARDRVAELERARKEPIAIVGLAC
                     RFPGGADTPERYWELLAAGRDAVTEVPPERWSLDAV"
BASE COUNT          235 a          746 c          787 g          252 t
ORIGIN      
        1 cggcgcgcgc gccgatggga catggctcgt gctgcgcgac cgcggcgagg tcgcgacgga
       61 gctggtcgcg gccatcgagg cgcgtggcgg tcgcgcgttg ctcgtggacg acgacatcgc
      121 gagcccggag gtcgcccgga cgctgatcgc gagcgccgag tcgccgctgc gcggggtcgt
      181 ccacctccgc gggctcgact cgccggagat gccggccggc ggcctcgccg aactgcgcga
      241 cgccacgcgg cgcgattgcg gtgcgctcct gcacgtcgtg caggcgctcg ccgccgccga
      301 gaagccgcag ccgcagctct ggctcgccac gcgcggggtc cagcccgcgg gcggcgccgc
      361 cccggcgccg ctgcacgcgc cgctctgggg cctcggtcgc gtggtcgcgc tcgagcactc
      421 cgagctgtgg ggcggcatgg tcgacctgga cccggccggc atgcccggcg aggcggcgct
      481 gctgctcgac gagatcctcg cctccgacgg cgaggatcag gtcgcccacc gccgcggcga
      541 gcgctacgcc gcgcggctcg tgcccggagc gctcgacgcc ggccgcggcg tgctgctccg
      601 cccggacgcg agctacctgg tcgccggcgg ccgcggcgcg ctcggcctca agctcgccgc
      661 ctggatggcc gatcgcggcg cgcgtcatct gatcctcacc ggccgcggcc cggtgacgcg
      721 ggaagccgcc gcggcggtcg cgcgactcga ggaacgcggc gtgagcgtcg actgcccgcg
      781 cgcggacgtg gcggacccgg acgcgatggc cgccgtgctc gacgccgtgg agccgccggt
      841 gcgcggcgtc gtccacgcgg cgggcgtgtt cgagccgcgg cgggcggccg acatgtcgcg
      901 ggcggagttc gaggaggtcg ccgcggccaa gatcgagggc acctgggtgc tgcacgagct
      961 gacccgcgcc acggagctcg acttcttcgt gatgttctcg tcggccgcgt ccgtgtgggg
     1021 ctcggcgctc gccggccact acgtagccgg caaccacttc caggacgtcg ccgcgcacca
     1081 ccggcgcggc ctcgggcttc ccgccctcgc ggtcaactgg ggctggtggg aggggagcga
     1141 catggtcccg gaggaggcca aggcctactt cggcgcgctc ggtctggagg tcgtgccgga
     1201 ggacgccggc ttcgacgcgc tcgagcgcct gctcgagtcg aaggcggtcc agcggacgat
     1261 cgcgcccgtc gactggcgca ccttcaaggc agccttcagc gcgcgccggc ggcggccgct
     1321 gctcgatctg atcgaggtcc cggtggagcc gggcggcgcg gcctccgcgg aggggctgga
     1381 gctgatccgg gcgctcgagc aggcgcccgc caggcgcaga ggcgagctga tcgtcgattt
     1441 cctccagcgc ctggtcggcg cggcgctcgg tctcgaccgc ccgatggacc cgcagctcgg
     1501 cttcttcgac gcgggcatgg actcgatcac ggccgtcgag gtgaagacgc gcatcgagtc
     1561 cagcctcggg gtcgccatcc ccgccaccgc ggcgttcgag cacccgaaca tcgaagcgct
     1621 cgccacctac ctgctcggcg acgtgctcga gctggcggcg ccggagccgg tcgaggagaa
     1681 gccgccggag gcgaacggca acggtcacgc ggagctcgac ggactgtccg aggacgagct
     1741 gctcgacctg ctcgagaacc agctggcggg ggcggagcgg tgagcgcgcc ggcggaacgc
     1801 cgcgaggaga tcatccggcg ggcgctcgtc gagctgggcg aggcgcgcga ccgggtggcc
     1861 gagctcgagc gggcgcgcaa ggagccgatc gccatcgtcg gcctcgcctg ccgctttccc
     1921 ggcggcgccg acacccccga gcgctactgg gagctcctgg ccgccgggcg cgacgccgtc
     1981 accgaggtcc cgcccgagcg ctggtcgctc gacgccgtct
//
LOCUS       DASVPL010000067         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_282669, whole genome shotgun sequence.
ACCESSION   DASVPL010000067 DASVPL010000000
VERSION     DASVPL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282669"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VF517_03235"
     CDS             <1..305
                     /locus_tag="VF517_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8051978.1"
                     /translation="PLATALDRSTARLPLPEVRELAAAINRASRHGAPLGRTLAAQAA
                     EARAARRRRIEEEAARAGPKIQLVIALLLVPSVLLLVAAALASALLGGGGSLPVGP"
     gene            332..1000
                     /locus_tag="VF517_03240"
     CDS             332..1000
                     /locus_tag="VF517_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX8051979.1"
                     /translation="MLSARQLRTRFGPGARAGAPYALAAALVAASFGVLAEPVFGGFA
                     TVAFSAIVFAGSAQFAALAVLADGGGAGTAIVAGILLNARFVTMGIAFGPSTTGGWLA
                     RALQGQAVVDASWALANRGGGRFDRDFLLGSTALAYPAWVLGTLVGVLVGDRLGSPQE
                     LGLDALFPAFFLGLLVEEIRDRVATAVALLGAVIALALTPIAPPGIPILAASLAVLIG
                     AWRR"
     gene            997..1305
                     /locus_tag="VF517_03245"
     CDS             997..1305
                     /locus_tag="VF517_03245"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX8051980.1"
                     /translation="MNAAWTTIAGLAVATFAIKASGPVALGGRSLPDWGLRLIALVAP
                     ALLGALVVVETFGRGKELVIDARVAGMVAAAAVLAARRSVLWAVAAAAVVTALVRALG
                     "
     gene            1609..2043
                     /gene="mraZ"
                     /locus_tag="VF517_03250"
     CDS             1609..2043
                     /gene="mraZ"
                     /locus_tag="VF517_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX8051981.1"
                     /translation="MAFRGHFDYSLDAKNRLNVPAKFRAAFSSGVVLAKGLEPCVAVW
                     APDTFERWTESFLSNLNPVSPERRKLTRYFAGSSFDAELDSAGRVTLNQALLEHAGIQ
                     KEVVIVGNLDHIELWDRDRWSEDQQALNAEVAGIAESLGHPS"
BASE COUNT          237 a          798 c          713 g          311 t
ORIGIN      
        1 tgccgctcgc caccgccctc gaccgctcca cggcccgtct gccgctcccg gaggtgcgcg
       61 agctcgccgc ggccatcaac cgcgcctcac gccacggcgc cccgctcggc cgcacgctcg
      121 ccgcccaggc ggccgaggcg cgcgccgcgc gccggcggcg gatcgaggag gaggccgcgc
      181 gcgccggtcc caagatccag ctcgtgatcg cgctgctgct cgtgccgtcc gtgctcctgc
      241 tggtcgcggc cgcgctcgcg tcggccctgc tcgggggagg agggtcgctg ccggtcgggc
      301 cctgagaacc cgcgctcgtc agactggcgc cgtgctcagc gctcgtcaac tccgcacgcg
      361 cttcggcccc ggcgcccggg cgggcgcccc ctacgcgctc gccgcagcgc tcgtcgccgc
      421 ctcgttcggc gtgctggccg agccggtgtt cggcgggttc gccacggtcg ccttctccgc
      481 gatcgtcttc gccggcagcg cccagttcgc cgcgctggcc gtgctcgccg acggcggcgg
      541 cgccggcacc gcgatcgtcg ccggcatcct cctcaacgcc cgcttcgtga cgatgggcat
      601 cgccttcggg ccctcgacca cgggcggctg gctcgcccgc gcgctccagg gccaggccgt
      661 cgtcgacgcc tcctgggcgc tcgccaaccg cggcggtggg cgcttcgacc gcgacttcct
      721 gctcggctcc acggcgctcg cctatcccgc atgggtgctg ggcacgctcg tgggggtcct
      781 ggtcggcgac cgcctcggca gcccccagga gctcggcctc gacgccctct tcccggcgtt
      841 cttcctggga ctgctcgtgg aggagatccg cgaccgcgtc gcgaccgcgg tggcgctgct
      901 gggggccgtg atcgccctgg cgctcacccc gatcgctccg ccgggcatcc cgatcctggc
      961 ggcctcgctg gccgtgctga tcggagcgtg gcgccggtga acgcggcttg gaccacgatc
     1021 gccggcctcg cggtcgcgac gttcgcgatc aaggcctccg gccctgtcgc gctggggggc
     1081 cgctcgctcc cggactgggg cctgcggctg atcgccctgg tcgcgcccgc cctcctcggc
     1141 gcgctggtgg tggtcgagac cttcggccgc gggaaggagc tcgtgatcga cgcgcgggtg
     1201 gccgggatgg tggccgcggc cgccgtgctc gcggcgcgcc ggtcggtgct ctgggcggtg
     1261 gcggccgcgg ccgtggtcac ggcgctggtc cgcgccctcg gctgacaggc ccctgggcgc
     1321 aaacgcccac ccaagcacgc cggatgcaat aaagcacccg gcctccgtgg gcccgcctcc
     1381 caccgtccga cccaccccgt ttaccgctct acagagcgta atccggtctc ccgcgcccac
     1441 cgagagacgc gcgttcggcg gctctgtgga caaaagtgtt gcaatgtggg gaggagtggg
     1501 ttacagtggc cccgccttca tggaccgggg tggcggagtt cctcccactc ggccactccg
     1561 gtccgaagaa cttcccccgc cgcacgatgc aaagggtcca ccacgcacat ggcgttccgc
     1621 gggcacttcg actactcgct cgacgcgaag aacaggctca acgtcccggc aaagttccgg
     1681 gcagccttct cgtccggagt ggttctcgcg aaggggctcg agccctgcgt ggcggtctgg
     1741 gcgccggaca ccttcgagcg ctggaccgag agcttcctct cgaacctgaa ccccgtcagc
     1801 cccgagcgcc gcaagctcac gcgctacttc gcgggcagct ccttcgacgc ggagctcgac
     1861 tcggccggcc gcgtgacgct caaccaggcg ctgctggagc acgccgggat ccagaaggag
     1921 gtcgtgatcg tcgggaacct cgatcacatc gagctctggg atcgcgaccg ctggagcgag
     1981 gaccagcagg ccctgaacgc ggaagtcgcc ggaatcgctg agagccttgg ccatccttct
     2041 tgacatgaca cgcacccac
//
LOCUS       DASVPL010000068         5902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_203684, whole genome shotgun sequence.
ACCESSION   DASVPL010000068 DASVPL010000000
VERSION     DASVPL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203684"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF517_03255"
     CDS             complement(<1..272)
                     /locus_tag="VF517_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051982.1"
                     /translation="MTTFLLDIWHDLREKRLWPVAVALLAAIVAIPAVLIKPAETPQV
                     DAAPPSATTAALPTVASDPSIVDGSNLDAFDIKDPFSSAADRTSGSV"
     gene            complement(269..1180)
                     /gene="pilO"
                     /locus_tag="VF517_03260"
     CDS             complement(269..1180)
                     /gene="pilO"
                     /locus_tag="VF517_03260"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX8051983.1"
                     /translation="MSLTPRDRKIVLILLPIVVLLGYWFMVLAPKRAESAKATEELTK
                     ARGERDTAQAQVGQLNAAKASFASDYQTVIRMGKAVPETVDMPSLLVQLDRAARGTGI
                     EMREIKVSPATASASAAPTSTPPGGGGTPAAGGSGAQSPMGKTAQGAGNAVNDANAKG
                     QAAGGDPAAGGGAGAAAPGAPAAGGQQAPGLSSVGLQFQLRGDFFDLADFFHRMKRFV
                     RVVNDQIVVRGRLIAIDSFSFTVDETGELEADVASTVYLSPKAAGGVSAGAGPTGPAA
                     APGGAGGTVAADPASQSTPPTAAAPTP"
     gene            complement(1177..1971)
                     /locus_tag="VF517_03265"
     CDS             complement(1177..1971)
                     /locus_tag="VF517_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051984.1"
                     /translation="MNAVNLLPAKHRPRTPTGGQAGSSYAVIGVLGALCVMVLLFVLT
                     VNGINSSKQAKSKTEAETAQAKKQATELTPYGNFITVKEQRVSSVKQLATGRIDWERL
                     TLGLAHVLPEDVWLMSATAAASGAPGQGGSQSAAPAAPAPAPGGGGATAAPSDPSGGA
                     AGPKPSLVLNGCAGSHSQVAVTLVRLRHLPAVEDVELASIQKPDNPGAVGGAASSGGG
                     GSTGEDCGTLKKKLAYKWEAKVTFKPQAGSTPTPEKKVPAALGGGA"
     gene            complement(1968..2903)
                     /gene="pilM"
                     /locus_tag="VF517_03270"
     CDS             complement(1968..2903)
                     /gene="pilM"
                     /locus_tag="VF517_03270"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX8051985.1"
                     /translation="MGLSMRKNNNRGTVGLDLDGGFISAVQATADGVARAASFDLAPG
                     VISDGEVTDVNALTEGLRDFFKETGLPRRVRLGIANQQIVVRQLDIPKMEEEHERAAA
                     VRFQAAEAIAMPLDEVVLDYQIIGEVQSPEGSPRLRVVVVAARESMISRIVEAVRDAG
                     LRPEGIDLNAFALVRTLAVPTDSTEETARVYCHLGGVTNLAIAIGTTCLFTRPLSAQW
                     DQDDDQVANTLAEEIRLSIDFYMAQPEARWVGEIVLSGPGSRREGLADSLSGLIEVPV
                     TVAEPLGRLQTAKLPSDEDPTRHTIAAGLALGAVA"
     gene            complement(2947..3693)
                     /locus_tag="VF517_03275"
     CDS             complement(2947..3693)
                     /locus_tag="VF517_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX8051986.1"
                     /translation="MNFALAAPLGLVIGSFLNVVAYRLPRGESLVTPGSHCTTCGTAV
                     KPWDNIPVLSWIFLRGRCRGCGDKISARYPLIELVTGAVFVLIAVVNGLDTDLVLELP
                     FAAMLITVAGIDLEHRIIPNKIVVPMAVFGIGATAVVRSDFLVEALIGGAAAFTFMFL
                     IALVHPKGMGMGDVKLAGVMGIFLGLSVVPALVLGFLTGSLVGVAVIARYGAAGRKRG
                     VPFAPFLALGGLVALVAGSELVDLYVKNFL"
     gene            complement(3853..4290)
                     /locus_tag="VF517_03280"
     CDS             complement(3853..4290)
                     /locus_tag="VF517_03280"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8051987.1"
                     /translation="MLRKLRNEESGFTLIELLVVILIIGILAAIALPAFLGQQEKGQD
                     ADAKSNARNLVSQVESCFATEQTYVSCDTAAELPNTGLSYGTGAGQVQVTASTSSTYT
                     ISAVSKAGGATPHTFTIQKTATGDSVRACTPTGKGGCPNSGAW"
     regulatory      complement(4357..4449)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4568..4966
                     /locus_tag="VF517_03285"
     CDS             4568..4966
                     /locus_tag="VF517_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051988.1"
                     /translation="MQLRRGLLTFALVLVAVSLGAALAAPDEEGEPATTTPQRTHSST
                     PNASVVTLRHPVEARAPVRRVRSGAHVVLRVTAGTAGSVEIPGLGLLEPVAPGSPATF
                     DLLASPGRHQVVLVTAGGERIPLGTLAVGD"
     gene            complement(4973..>5902)
                     /locus_tag="VF517_03290"
     CDS             complement(4973..>5902)
                     /locus_tag="VF517_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX8051989.1"
                     /translation="FQQYRSLFDRTPAAVLGLLLVALTILVLLLEARARGRARVASSS
                     RGTPREATPVALGRWRWPALAICATVVAAALVLPVAVLVYWVTRGVEGDVVDAMWLAA
                     RNSLLASGLAAGAAILAALPVAALVVRHPARWTRALERTSYASNALPGIVIALSLVFF
                     AANYASPIYQTLALLVIAYVVRFFPQALAGAHGALLRVDPRLEEAARGLGRTPRGAFA
                     SITARLIAPGLLAGATLVFLSAMKELPATLLLRPIGFETLATEVWSATGVGSYSQAAV
                     PALVLIVLAAPVVWLLIARPDPATARVPTDPPE"
BASE COUNT          850 a         1989 c         2233 g          830 t
ORIGIN      
        1 accgagcccg aggtgcggtc ggcggcggag ctgaacggat ccttgatgtc gaaggcgtcg
       61 aggttcgagc cgtcgacgat gctcgggtcg ctggcgaccg tcggcagcgc cgcggtggtc
      121 gcgctcgggg gagccgcgtc cacctgcggg gtctcggcgg gcttgatcag caccgccggg
      181 atcgcgacga tcgcggccag gagcgcgacg gccaccggcc agaggcgctt ctcgcggagg
      241 tcatgccaga tgtcgagcag gaaagtggtc atggggtggg ggctgctgcg gtcgggggag
      301 tggactgcga agcggggtcg gccgcgacgg tgccaccggc gccgccggga gctgccgcgg
      361 ggccggtcgg gccggcgccg gcggatacgc cacccgcggc cttggggctg aggtagacgg
      421 tcgaggcgac gtccgcctcg agctcgccgg tctcgtcgac cgtgaagctg aagctgtcga
      481 tcgcgatcag gcggccgcgg accacgatct ggtcgttgac cacccggacg aagcgcttca
      541 tccggtggaa gaagtcggcg aggtcgaaga agtcgccgcg aagctggaac tggaggccga
      601 ccgacgagag gccgggagcc tgctggccgc ccgccgcggg agcaccgggc gcagccgcgc
      661 ccgcgccgcc gccggccgcc gggtcgccgc cagctgcctg gcccttcgcg ttcgcgtcgt
      721 tgaccgcgtt gcccgcgccc tgggcggtct tgcccatcgg gctctgagcg ccgctcccgc
      781 cggccgcggg ggtgccgccg ccgcccgggg gcgtcgaggt cggggccgcg ctggccgagg
      841 ccgtggcggg gctgaccttg atctcacgca tttcgatgcc cgtgccgcgc gcggcccggt
      901 cgagctgcac caggaggctc ggcatgtcca cggtctcggg gacggccttg cccatccgga
      961 tgacggtctg gtagtcgctg gcgaagctgg ccttggcggc gttgagctgc ccgacctgcg
     1021 cctgcgcggt gtcgcgctcg ccacgggcct tggtcagctc ctcggtcgcc ttggccgact
     1081 cggcgcgctt gggcgcgagg accatgaacc agtagccgag cagaacgacg atcggcagca
     1141 ggatcagcac gatcttgcgg tcacgcggtg tcaggctcat gctccacctc cgagcgccgc
     1201 cgggaccttc ttctccgggg tgggtgtgga gcccgcctgc ggcttgaagg tgaccttggc
     1261 ctcccacttg tatgcaagct tcttcttgag ggtcccgcag tcctcaccgg tgctgccgcc
     1321 gccaccggag gaggcggcac cgcccacggc gccggggttg tccggcttct ggatgctggc
     1381 gagctcgacg tcctcgaccg ccggcaggtg ccggagccgg acgagcgtga ccgcgacctg
     1441 ggagtggctc cccgcgcagc cgttgagcac gaggctgggc ttggggccgg ccgcgccgcc
     1501 cgaggggtcg gagggagccg ccgtcgcgcc gccgccaccg ggagccggag cgggggcggc
     1561 cggggccgcg ctctgggagc cgccctgacc gggagcgccg ctcgcggccg ccgtggccga
     1621 catcagccac acgtcctcgg ggagcacgtg cgcgagtccg agcgtgagcc gctcccagtc
     1681 gatgcgaccg gtcgcgagct gcttgaccga ggagacgcgc tgctccttga ccgtgatgaa
     1741 gttgccgtac ggggtgagct cggtggcctg cttcttggcc tgagccgtct cggcctcggt
     1801 cttcgacttc gcctgcttgc tggagttgat gccgttcacc gtgagcacga agaggaggac
     1861 catcacgcag agggcgccga gcacgccgat gacggcgtac gagctgcccg cctggccgcc
     1921 ggtgggcgtc cgcggacggt gcttcgcggg gagaaggttg actgcgttca tgccacggcc
     1981 cccagcgcga gacccgcggc gatggtgtgc cgggtcggat cctcgtcgga cggaagcttg
     2041 gcggtctgga gacggccgag cggctcggcc acggtgaccg ggacctcgat gaggccgctg
     2101 agcgagtcgg ccaggccctc acggcgcgag ccggggccgg agaggacgat ctcgcccacc
     2161 cagcgcgcct cgggctgcgc catgtagaag tcgatcgaca ggcggatctc ctcggcgagg
     2221 gtgttggcga cctgatcgtc gtcctggtcc cactgggccg agagcggccg cgtgaagagg
     2281 caggtcgtgc cgatcgcgat ggcgaggttg gtgacgccgc cgaggtggca gtagacgcgc
     2341 gcggtctcct cggtggagtc ggtcggcacg gcgagcgtgc ggaccagcgc gaaggcgttc
     2401 aggtcgatcc cctcggggcg caggccggcg tcgcgcacgg cctcgacgat gcgggagatc
     2461 atcgactcgc gggcggcgac gacgacgacg cggaggcgcg gcgagccctc ggggctctga
     2521 acctcgccga tgatctggta gtcgagcacg acctcgtcga gcggcatcgc gatggcctcg
     2581 gcggcctgga agcggacggc ggcggcgcgc tcgtgctcct cctccatctt cgggatgtcg
     2641 agctggcgga cgacgatctg ctggttggcg atgccgaggc gcacgcggcg cggtaggccg
     2701 gtctccttga agaagtcgcg aaggccctcg gtcagcgcgt tgacgtcggt gacctcaccg
     2761 tcgctgatga ctcccggggc gaggtcgaag ctcgcggcgc gagcgacgcc gtcggcggtg
     2821 gcctgaacgg ccgagatgaa gccgccgtcg aggtcgaggc ctacggttcc gcggttgttg
     2881 ttcttgcgca tcgagagtcc catgaggttc cttacaagcg aggtatcgac cgttcgctga
     2941 cgggctttag aggaagttct tgacgtagag atcgacgagc tcggagcccg ccacgagcgc
     3001 caccaacccg cccagggcga ggaagggtgc gaacggcacg ccgcgcttgc gaccggcggc
     3061 tccgtacctg gcgatgaccg ccacgccgac gagcgatccg gttaggaagc cgagcaccag
     3121 agccggcacc acggacaggc cgaggaagat ccccatcacg cccgcgagct tcacgtcgcc
     3181 catgcccatg cccttcgggt ggacgagcgc gatcaggaac atgaaggtga acgcggcggc
     3241 tccgccgatc agcgcctcga cgaggaagtc cgaacgcacg accgcggtcg cgccgatgcc
     3301 gaagaccgcc atcgggacga caatcttgtt cgggatgatc cggtgctcga ggtcgatgcc
     3361 cgcgaccgtg atcagcatcg ccgcgaacgg cagctcgagc acgaggtcgg tgtcgaggcc
     3421 gttgaccacc gcgatcagga cgaacaccgc gccggtgacc agctcgatca gcgggtagcg
     3481 ggcggagatc ttgtcgccgc agccgcggca gcgcccacgc aggaagatcc acgagaggac
     3541 cgggatgttg tcccagggct tgaccgccgt gccgcatgtg gtgcagtgcg agccgggcgt
     3601 gacgagcgac tccccgcgcg gcagccggta ggccaccacg ttcaggaacg agccgatcac
     3661 caggccgaga ggggcagcga gtgcgaagtt catgtgaggg tctaaggagg gggagggtga
     3721 tactcgccgg ggggcgagtg gaggtacgga cctgatcggc gcgaggcgcg cgcggcttga
     3781 gcgggccgtg aaaggcgaga ggcgggcccg ggggcccgcc tctcggaaac tgccggttga
     3841 acctttcggt tcttaccagg cgccgctgtt ggggcagccg cccttgccgg tcggggtgca
     3901 ggcgcggacg ctgtcgcccg tcgcggtctt ctggatggtg aaggtgtgcg gcgtggcgcc
     3961 acccgccttc gagacggccg agatcgtgta ggtgctggag gtggaggcgg ttacctgcac
     4021 ctggccagcg ccagtgccgt acgagaggcc cgtgttgggc agctcggcgg ccgtgtcaca
     4081 cgacacgtac gtctgctcgg tcgcgaagca cgactcgacc tgggacacca ggttacgggc
     4141 gttggacttc gcgtccgcgt cctggccctt ctcctgctgg ccgaggaagg ccggcagagc
     4201 gatcgcggca aggatgccga tgatcaggat tacgacgagg agctcgataa gcgtaaaacc
     4261 gctctcctcg ttgcggagct tccgcagcat atgactctcc ttgagtccga attgcattta
     4321 cgccagctcc gcggatagcc ccggagactt tcgtcccgtg gtaacccggc ttgctcttgg
     4381 gtctgagcct caccctcgag ccccgtggct ttgcgccccc gcctcacgac gggtttgcct
     4441 ttcacaccgt ggcctggctt ctgtagccgg ttatcgactg caccccccgg cgcctttaac
     4501 ttcatttgag gtcgccccgc ccgtcatccg tccagtgcgc tccggcgccg caccgtacga
     4561 tcggcggatg cagctccgtc gcggccttct cacattcgcg ctggtgctcg tcgcggtcag
     4621 cctcggggcg gccctggcgg cgcccgacga ggagggcgag ccggccacga caacgccgca
     4681 gcggacgcac tcgagcaccc cgaacgcgag cgtggtgacg ctgcgtcacc cggtcgaggc
     4741 cagggcgccg gtgaggcggg tgcgcagcgg cgcgcacgtg gtgctgcggg tcacggcggg
     4801 cacggcgggc agcgtggaga tcccgggcct cgggctgctc gagccggtcg cgcccggctc
     4861 cccggcgacc ttcgacctgc tcgcctcccc cgggcgccac caggtcgtgc tggtcacggc
     4921 gggcggtgag cggatcccgc tcgggacgct cgccgtcggc gactaacggt cgctactccg
     4981 gcgggtcggt gggcacccgc gcggtggccg ggtcgggccg cgcgatcagc agccagacga
     5041 ccggggccgc gagcacgatc agtacgagcg cgggcacggc ggcctgcgag tacgagccga
     5101 cgccggtggc gctccacacc tcggtcgcga gcgtctcgaa gccgatcggg cgcagcagca
     5161 gcgtggccgg cagctccttc atcgccgaga ggaacacgag cgtcgcaccc gcgagcagtc
     5221 cgggcgcgat caggcgggcg gtgatcgacg cgaacgcgcc gcgcggcgtg cgcccgagcc
     5281 cgcgggccgc ctcctcgagt cgcgggtcga cgcgcagcag agcaccgtgc gcgcccgcca
     5341 gcgcctgggg aaagaagcgg accacgtagg cgatcacgag cagcgcgagc gtctggtaga
     5401 tcggcgaggc gtagttggcg gcgaaaaaga cgagcgacag cgcgatcacg atgccgggca
     5461 gcgcgttcga cgcgtacgag gtgcgctcga gagcgcgtgt ccagcgcgcc ggatggcgga
     5521 ccacgagcgc cgcgaccggc agggcggcaa ggatcgcggc cccggccgcg aggcccgacg
     5581 ccagcagcga gttgcgcgcc gcgagccaca tggcgtccac cacgtcgccc tccaccccgc
     5641 gcgtgaccca gtagacgagc accgccaccg gcaggacgag tgcggctgcc acgaccgtcg
     5701 cgcagatcgc gagcgccggc cagcgccagc ggccgagcgc gaccggggtc gcctcgcgcg
     5761 gcgtgccccg tgaggagctg gccacgcgcg cgcggccgcg ggcccgcgcc tccagcagca
     5821 gtacgaggat cgtgagcgcc acgagcagca gcccgagcac ggccgccggc gtcctgtcga
     5881 acagcgagcg gtactgctgg aa
//
LOCUS       DASVPL010000069         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_282916, whole genome shotgun sequence.
ACCESSION   DASVPL010000069 DASVPL010000000
VERSION     DASVPL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282916"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..659
                     /locus_tag="VF517_03295"
     CDS             153..659
                     /locus_tag="VF517_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8051990.1"
                     /translation="MELVRGLPPARDALDLGAGDGRLSGEIAAERLVLADVSRVALER
                     AARRHPGARTEPLAPDEPLPFADSAFDLVVCVNVLNHVRDVQLFVSEARRVLRPGGRL
                     AVTTAAHGRATALAILARGFERPFDPLSPHLRFLTGRSLARLLGDMGFEVESLRRRRG
                     DLLAVARR"
     gene            complement(656..>1838)
                     /locus_tag="VF517_03300"
     CDS             complement(656..>1838)
                     /locus_tag="VF517_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8051991.1"
                     /translation="GRYLGAVSMVADMTEHHVARTERERLENRLQQSQRLETVGQLAG
                     GVAHDFNNLLAVILNYAYFVRERLPEDSEVRADLEEIRHAAERASELTHQLLVFSRRE
                     TIRPVVLDLNDVVRDMQRLLGRTLGERVELVTELADEPCLVEADSSQLEQVVLNLVVN
                     ARDAMPRGGTIRVETRLGEEVLLAVSDEGEGMEPEVAARAFEPFFTTKPKGAGTGLGL
                     ATVYGTVTQAGGHAEIDSEPGRGTTLRVYLPQAAPRESDAPEVVAVDSSPTDGATVLV
                     VEDEDPVRRLTCRILAGSGYECLEARGGEEALRLYEQHRSRVALLLSDVVMPGMSGQE
                     LAQRIGGEGSGPPVLFMSGYTDEAVLPRERADEGALLHKPFTADALLRSVREALREDR
                     D"
BASE COUNT          209 a          699 c          674 g          256 t
ORIGIN      
        1 tacctcgagc gctggtcggt cttcctcgac ctgtcgatcc tgctgaagac gatccccgcg
       61 gtgatcaggg cgaagggcgc ctggtagccc gctacgacga cgagctctgg gagctcgtgc
      121 cggaggatcg cgggccggcc ccggaccacc tcgtggagct cgtgcgcggc ctgccgcccg
      181 cgcgcgacgc gctcgacctg ggcgccggcg acgggcgcct gtctggcgag atcgcggccg
      241 agcgcctcgt gctggcggac gtctcgcgcg tcgccctcga gcgggcggcg cggcgccatc
      301 cgggcgcacg caccgagccg ctcgcgccgg acgagccgct gccgttcgcc gactcggcgt
      361 tcgacctcgt cgtctgcgtg aacgtgctta accacgtccg ggacgtgcag ctcttcgtgt
      421 ccgaggcgcg ccgcgtgctg cggcccggcg ggcgcctggc cgtgaccacc gccgcgcacg
      481 ggcgcgcgac ggcgctcgcg atcctcgccc gcggcttcga gcgcccgttc gacccgctgt
      541 cgccgcacct gcgcttcctc accggccggt ccctggcgcg gctgctcggc gacatggggt
      601 tcgaggtcga gagcctgcgc cggcggcgcg gcgacctgct ggccgtcgcc cggcgctagt
      661 cgcggtcctc gcgcagcgcc tcgcgcacgc tccggaggag cgcgtcggcc gtgaacggct
      721 tgtggaggag tgcgccctcg tccgcccgct cgcgcggcag gaccgcctcg tcggtgtagc
      781 cggacatgaa cagcaccggc gggccggacc cctcgccgcc gatccgctgg gccagctcct
      841 ggccggacat gccgggcatc acgacgtcgg agaggagcag cgcgacgcgg ctgcggtgct
      901 gctcgtacag gcgcagcgcc tcctcgccgc cgcgcgcctc gaggcactcg tacccgctgc
      961 cggcgaggat ccggcaggtc agccggcgca cggggtcctc gtcctcgacg accaggaccg
     1021 tcgcgccgtc ggtcggcgag ctgtccacgg ccaccacctc cggggcgtcg gactcgcgcg
     1081 gcgcggcctg cgggaggtag acgcgcaggg tggtgccgcg acccggctcg gagtcgatct
     1141 cggcgtgccc gccggcctgc gtcacggtgc cgtagacggt cgcgagcccg agccccgtgc
     1201 cggcgccctt gggcttggtc gtgaagaacg gctcgaacgc gcgcgccgcc acctcgggct
     1261 ccatgccctc gccctcgtcg gagacggcaa ggagcacctc ctccccgagc cgggtctcga
     1321 cccggatcgt gccgccgcgc ggcatcgcgt cgcgcgcgtt gacgaccagg ttcagcacga
     1381 cctgctcgag ctgcgacgag tcggcctcga ccagacaggg ctcgtcggcc agctccgtga
     1441 cgagctcgac gcgctcgccg agcgtccgcc cgagcagccg ctgcatgtcg cgcacgacgt
     1501 cgttcaggtc gagcaccacc ggccggatgg tctcgcggcg gctgaagacc agcagctggt
     1561 gggtcagctc cgaggcgcgc tcggcggcgt ggcggatctc ctcgaggtcg gcccggacct
     1621 cgctgtcctc gggcagccgc tcgcggacga agtaggcgta gttgaggatc accgcgagca
     1681 ggttgttgaa gtcgtgggcc accccgccgg cgagctgccc gaccgtctcg agccgctgcg
     1741 actgctgcag gcggttctcg agccgctcgc gctcggtccg cgcgacgtgg tgctcggtca
     1801 tgtcggcgac catcgacacc gcgcccaggt agcgcccg
//
LOCUS       DASVPL010000070         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_362294, whole genome shotgun sequence.
ACCESSION   DASVPL010000070 DASVPL010000000
VERSION     DASVPL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362294"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VF517_03305"
     CDS             <1..555
                     /locus_tag="VF517_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051992.1"
                     /translation="GLGRRAPLDATLGDAGSFASYLWQFYLPKLSFQDQFPGLGERPL
                     WNVWHKTSWGAFGQLEVTLPDAAYVILAVVTVAVAAAAVAAVVRRRVRPDRMAAAFLG
                     LFAVVLLLGLHWVEFRSLGRIEGTTNQGRYLLPLMPIVAVAVAAGLTNLPSRLRAAGA
                     GLVLAGMFALQLLSLAVVAGRFYA"
     gene            548..1231
                     /locus_tag="VF517_03310"
     CDS             548..1231
                     /locus_tag="VF517_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8051993.1"
                     /translation="MRSGVAALAAGCAIALAVLAVVAAGDRRERSFGPNVRAERVLAV
                     ATPAREICQSDVGVAADFDTVELLLGTYGRPGPPLAVRVRDTRSGGQLAAGTLPAGAA
                     DNRAARVRVEPEVERGGSVDVCVRTAARRVALYGGADSDVITEARAGGRRVDGDLWIA
                     FGRSEPRSALALLPDMLRRASVFRPEPVGPWAFWVLLAGVVAGVPLLLAGALRRAAGG
                     TAAKRANSR"
     gene            1397..2992
                     /locus_tag="VF517_03315"
     CDS             1397..2992
                     /locus_tag="VF517_03315"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8051994.1"
                     /translation="MSEGQLADAPAIDVTEPTAPPLTGKRRFVHDARTRAGGGMTSPL
                     RIPAVRRATTDVRALEAEARSTSTPIREGDRAAETLDRDALHRRALAVADMFSAAAVL
                     LGAVLIGGGTPRFGLVAILPVVVLISKVVGLYDRDEHVLHKTTLDETPKLFHVALTFA
                     FLAFLADDLLATHGFDKPEILFTLGALVILLGANRSLARRLALRAATAERILVLGSAV
                     ECRRIEEKLDTVSPVKAEIVGRVPVSHEPRSERGDEVLGMLPELDFVLRRERIDRVVI
                     CPHGDSSTDMLDTIRTVKGLGVKVSVMPRLFEVVGSSVEFDELGGMTLLGIRRYELTK
                     SSAFLKRALDVAGASVGLVLLAPFFITMAIAIKVGSPGHVFFRQRRVGRGGTAFDMFK
                     FRTMYAGADRAKADLLDLNEADGFFKIADDPRVTGIGRILRRSSLDELPQLINVLRGE
                     MSLVGPRPLVYEEDARIEGFSRRRLDVTPGMTGAWQVLGSSRVPMRDMITIDYLYRAN
                     WSLWLDLKILLRTVPHVVRRRGM"
BASE COUNT          382 a         1103 c         1118 g          474 t
ORIGIN      
        1 gggctcggtc gccgcgcgcc gctcgacgcg acgctcggcg acgcgggcag cttcgccagc
       61 tacctgtggc agttctacct gcccaagctc tcgttccagg accagttccc cggtctcggc
      121 gagcggccgc tctggaacgt ctggcacaag acctcgtggg gtgcgttcgg gcagctcgag
      181 gtcacgctgc cggacgcggc gtacgtgatc ctcgcggtgg tgaccgtcgc cgtggctgcg
      241 gccgccgtgg cggcggtcgt tcgccggcgg gtccggcccg atcgaatggc ggcggcgttc
      301 ctcgggctct tcgcggtggt gctgctcctc ggcctgcact gggtcgagtt ccgctcgctc
      361 ggccggatcg agggaaccac gaaccagggt cgctacctgc tcccgctgat gccgatcgtc
      421 gccgtcgccg tcgccgcggg gctgacgaac ctaccgtcgc ggctgcgcgc cgccggcgcc
      481 ggcctggtcc tcgcggggat gttcgcgctt cagctcctct cgctcgccgt cgtggcgggg
      541 cgcttctatg cgtagcggcg tcgccgcact ggccgccggc tgcgccatcg ccctcgcggt
      601 cctggcggtc gtggcagccg gcgaccggcg cgagcgctcg ttcggcccga acgtgcgcgc
      661 cgagcgcgtg ctcgcggtgg ccacgcccgc gcgggagatc tgccagagcg acgtcggcgt
      721 ggccgcggac ttcgacaccg tcgagctcct gctcggcacg tacggccgcc ccggcccgcc
      781 gctcgcggtg agggtccgcg acacgcgcag cggcgggcag ctcgcggcgg gcacgctccc
      841 cgccggggcg gccgacaacc gcgcggcgcg tgtgcgcgtg gagcccgagg tggagcgcgg
      901 cggcagcgtc gatgtctgcg tgcgaacagc ggcgcgccgc gttgcgctgt acggcggtgc
      961 ggacagcgac gtcatcaccg aggcccgcgc gggcgggcgg cgcgtggacg gcgatctgtg
     1021 gatcgcattc ggccgcagcg agccgcgcag cgcgctcgcg ctcctgcccg acatgctccg
     1081 gcgagcctcg gtgttccgcc ccgagccggt cggcccgtgg gcgttctggg tgctgctcgc
     1141 gggggtcgtg gccggggtcc cgctgctgct cgccggggcc cttcggaggg ccgccggcgg
     1201 aaccgctgcc aaacgggcga attcgcgcta ggctggcccg agcccaggca gggtgagagg
     1261 gagcagccaa ggattcggtt tctccacccc ccactcaggg gatagggggg ttcagccgca
     1321 agaagcggct ggggggacag ccggtggtgc ggcgacctct ggaattccgg actagcgacc
     1381 ggggggactg tgaggcatga gtgaggggca actggcggat gcgccagcga tcgatgtgac
     1441 ggagccgacg gctccgccgc tgacggggaa gcggcgcttc gtgcacgacg cgcggacccg
     1501 cgcaggcggt ggcatgacct cgccgctgcg catcccggcg gtgcggcgcg cgacgaccga
     1561 cgtccgcgcg ctcgaggccg aggcacgcag cacctccacg ccgattcgcg agggcgatcg
     1621 cgccgccgag accctcgatc gcgacgcgct tcaccggcgc gccctggcgg tcgccgacat
     1681 gttctccgcc gcggcggtcc tgctcggcgc cgtcctcatc ggcggtggca cgccccgatt
     1741 cggcctcgtg gcgatcctgc cggtcgtcgt gctgatcagc aaggtcgtcg gcctctacga
     1801 ccgcgacgag cacgtcctcc acaagacgac cctcgacgag acgcccaagc tcttccacgt
     1861 cgccctcacc ttcgcgttcc tcgcgtttct agcggacgac ctgctcgcga cccacggctt
     1921 cgacaagccg gagatcctct tcacgctcgg cgcgctcgtg atcctgctcg gcgccaaccg
     1981 ctcgctcgcg cgtcgcctcg cgctccgcgc cgccaccgcc gagcggatcc tcgtgctcgg
     2041 cagcgcggtc gagtgccgcc ggatcgagga gaagctcgac acggtctccc cggtgaaggc
     2101 cgagatcgtc gggcgcgtcc cggtgtcgca cgagccgcgc tcggagcgcg gcgacgaggt
     2161 cctcggcatg ctccccgagc tcgacttcgt cctgcgccgc gagcggatcg accgcgtcgt
     2221 gatctgcccg cacggcgaca gctcgaccga catgctcgac acgatccgca ccgtcaaggg
     2281 cctgggcgtg aaggtcagcg tgatgcctcg cctgttcgag gtggtcggct cgtcggtcga
     2341 gttcgacgag ctcggcggga tgacgctgct gggcatccgc cgctacgagc tgacgaagtc
     2401 ctcggccttc ctgaagcgcg cgctcgacgt ggcgggggcc tccgtcggcc tcgtgctgct
     2461 ggcgccgttc ttcatcacga tggcgatcgc gatcaaggtc ggctcgccgg gacacgtgtt
     2521 cttccgccag cgccgcgtcg gccgcggcgg caccgcgttc gacatgttca agttccgcac
     2581 gatgtacgcc ggcgccgacc gcgcgaaggc ggacctgctc gacctcaacg aggccgacgg
     2641 cttcttcaag atcgcggacg acccccgcgt gacggggatc ggccgcattc tgcggcgcag
     2701 ctcgctcgac gagcttcccc agctcatcaa cgtgctgcgc ggcgagatga gcctggtcgg
     2761 cccgcgtccg ctggtctacg aggaggacgc tcggatcgag ggcttctcgc ggcgccggct
     2821 cgacgtgact ccgggcatga cgggcgcctg gcaggtgctc ggcagctcgc gggtcccgat
     2881 gcgggacatg atcacgatcg actacctcta tcgcgcgaac tggtcgctct ggctcgacct
     2941 caagatcctg ctgaggaccg ttccccacgt ggtgcgccga cgcggtatgt gacactgagg
     3001 ggcatgagcc ccaaggtcga gaagtcagat caggagtggc gtgagcagct cacgccggag
     3061 cagtacgagg tgcttcg
//
LOCUS       DASVPL010000071        18861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_283258, whole genome shotgun sequence.
ACCESSION   DASVPL010000071 DASVPL010000000
VERSION     DASVPL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18861
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283258"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /gene="rbfA"
                     /locus_tag="VF517_03320"
     CDS             <1..222
                     /gene="rbfA"
                     /locus_tag="VF517_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX8051995.1"
                     /translation="DLRHARVYVSVLGSEVEREAALDGLTSARGFLQSQVGTELRMKR
                     TPTLEFVNDPSIEHGMKMERLISEVTDEG"
     gene            219..1187
                     /locus_tag="VF517_03325"
     CDS             219..1187
                     /locus_tag="VF517_03325"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX8051996.1"
                     /translation="MSDDRTQVLDELRAADKLLLTTHENPDGDALGSLRATQIVMEAL
                     GKDSEMFMAADNFPLPHEYRHMPFDDVLNEPPADLSERLVVFLDCGNIDRMPIDLTGG
                     VRIVNIDHHHDNTRFGDVNYVVADASCTAQIVHDLARELGVTIDKPLAEALYIALVTD
                     TGKFMFENTTPAAHQMAADLIAAGVQPQEVHQRLYEGLPFSRIQLLARALAAVQRFDD
                     GSITLTHLTIDDFNHSGAKESDSEGIVDHMRQVEGTLVAALVRELLEDNGRRKVSLRA
                     TNGRVDVSKIARGFGGGGHRQAAGFTTDVELPEMIERLRTEVNAQL"
     gene            1184..2008
                     /gene="truB"
                     /locus_tag="VF517_03330"
     CDS             1184..2008
                     /gene="truB"
                     /locus_tag="VF517_03330"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX8051997.1"
                     /translation="MNGVVLFAKPAGVTSHDVVAQVRRSLGCKAGHAGTLDPFATGLL
                     LVLVGRATRVQRFLMALPKTYRVTARLGWTSDTGDRDGALAHTGRVPETLSIPVGEIL
                     QRPPAYSAVKVGGERLYARARRGDPVEGEPRPVTVHRAELLWHEGERAGFEIECSSGT
                     YVRQLVTGLGDAYCEELERTAIGGFRLEDADPERVVPLEEALGFMPEAPLGEEDAAKV
                     SHGVGVSHGVGVSAPADGSAGPASSTEAVRLTHGGRLLAIGRPEGDLLKPFVVFPE"
     gene            2005..2892
                     /gene="ribF"
                     /locus_tag="VF517_03335"
     CDS             2005..2892
                     /gene="ribF"
                     /locus_tag="VF517_03335"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEX8051998.1"
                     /translation="MKVTSLPDAGRRPDGPRRVAVGTFDGVHLGHRAVIEGCDTVLTF
                     DPHPLAVIAPHALPKLLDSFPIKRDLIAGLGVEELVVIPFDAEFATRSAEDFIDNELV
                     ERLGAEVVCVGENFRFGKGARGDAAMLDAHPAFETRVVPMVEAAGETVSSTHIRGLVL
                     AGEVARAAEFLGAPFMMEGEVVHGDKRGRELGMPTANLVPDPRLAVPGHGVYAAWAHG
                     YPAAVNVGIRPTFDTGRGLLIEAYLLDFDGDLYGQTLRIAFAERLRGEKRFDSVDGLV
                     EQMQRDVEHVRRAANFARR"
     gene            2889..3158
                     /gene="rpsO"
                     /locus_tag="VF517_03340"
     CDS             2889..3158
                     /gene="rpsO"
                     /locus_tag="VF517_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX8051999.1"
                     /translation="MSLTIDAKREIIQKHGKTEADTGSPEVQIALLSARISHLTEHLR
                     EHKHDHHSRRGLLMLVGQRRRLLNYLQRKDLDRYRALIAELGLRR"
     gene            3155..3619
                     /locus_tag="VF517_03345"
     CDS             3155..3619
                     /locus_tag="VF517_03345"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8052000.1"
                     /translation="MIEAGTPAPDFALPDQDGNEVTLESLRGRRVVLVFYPADFSPVC
                     TDQLNVYQEVLGELKERGAELYGVSVDSNWCHRAFREHLGVEIPLLADFHPKAAVSQA
                     YGVYAEKYGVSLRALVMIGPDGTVEWTHVSPSPLEIPGANLIFDALEAHAAA"
     gene            3663..4109
                     /locus_tag="VF517_03350"
     CDS             3663..4109
                     /locus_tag="VF517_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8052001.1"
                     /translation="MRGDGPLVVEYGDYQCPYCAQADALLAELPVRRVFRHFPVVSKH
                     PRARVLAAAAEAAGIQGAFWEFHDSLYADQGRLDDPHLWSRVESLGLDLDRFEADRRA
                     EAVAERVGRDFKSAIRAGVTTTPTLFVDGVAHPGVPDPELLARLRG"
     gene            complement(4158..4439)
                     /locus_tag="VF517_03355"
     CDS             complement(4158..4439)
                     /locus_tag="VF517_03355"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX8052002.1"
                     /translation="MPEALRHVTAVELVGNHRLRLAFDDGAEGVIDASHWSWHGVFAP
                     LEDPEYFARVELDEGLGTIVWPNGADVAPETLHADATGTAPNPLTHRRE"
     gene            4847..7177
                     /locus_tag="VF517_03360"
     CDS             4847..7177
                     /locus_tag="VF517_03360"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX8052003.1"
                     /translation="MNQDVTRVSVEIGGTELSFETGKLAKQASGSVVVQAGDTMVLGT
                     ATAGNERDVDFLPLTVDVEERMYAAGKIPGSFFKREGRAGEKGTLTARMIDRPLRPLF
                     PKGWHRETQLVSIPMSVDHVHPYDILAMNGASAALIISDIPFDTPVGAVRIGQDEEGG
                     FIVNPDEEWLLETPLDLIVAGTEEAILMVEAGAKEVSEAVILDALDIAHGEIKKLCAL
                     QRELQQKVGKEKIVLEAPKVDESVLSQLKDQFGAKLDEATQVTDKLERQDATKAVETE
                     AVEALAGDPDAEDHAQKRAAAQLAFDKLEKEVIRRRIAVDKKRPDGRSAEEIRDIWIE
                     VGVSPRAHGSAVFTRGQTQAFSVAALGTTREEMRLDTLGLETTKHYFHHYNFPPFSVG
                     EAGFMRGPKRRDIGHGALAERALVPVVPSTEDFPYTIRVVSDILESNGSSSMASVCGS
                     SLSLMQAGVPLKAPVAGIAMGLIKEGDDYVVLSDIAGVEDHLGDMDFKVAGTEQGITA
                     LQMDIKITGVTFEILSDALAQAKQGRTHILGKMNDVISEPSSELSPHAPRITSIQIDT
                     DKIGMVIGKGGETIRALSEEFDAQIDMQDDGMVLVYASNGEQGDALVERIRSMTKDVE
                     VGDEFTGKVVKTTTFGAFVELSKGTDGLLHISNLKPGERVESVEDVVNRGDEVSVKVV
                     EVDRERGRIGLRMADDPDVAGKSPEELANVGTGGGGGGGGGGRDRDRGGRGGGDRGGR
                     GGGRGRDGDRGGRGSRGGDRGERGGGDRPNYARDER"
     gene            7236..8468
                     /locus_tag="VF517_03365"
     CDS             7236..8468
                     /locus_tag="VF517_03365"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8052004.1"
                     /translation="MSERLPSVRSIALGLFVGTGSRNETPEQGGISHFLEHLLFKGTD
                     RFSSIEIDQTFDGMGAEANAGTGKETTSVYSRFLDQHLERAYDVLADMVLRPAYPDID
                     SERQVVIEEIAMYEDEPADKVHDVLAQALFGDHPLGRPIIGTADVIANVPVPDIASYH
                     DSRYVGPNLVVSAAGNVDHGRLVELTERFFTAPTGDADGPTAAPASCPPRAAFHEKTT
                     EQYHLCLGGPGIARHDDRRFALRVLDAILGGSSSSRLFQEVREKRGLAYSVYSYASHF
                     ADAGQVGVYVGTRPDNVREAMDVIGTELRRIVEEPVAGDELERAKENVTGRMVLSFES
                     TLTRMNRLGGSVLMDVPLLSLDEMIAAVDAVDLDAVSELSRELFAPEKLSAAGVGGSE
                     DAFRAALEPVSGELAAAA"
     gene            8465..9121
                     /locus_tag="VF517_03370"
     CDS             8465..9121
                     /locus_tag="VF517_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8052005.1"
                     /translation="MINVAVSGAAGRMGQAVVAAVEGADDMALVGKADPLFEQSLQDV
                     LGDADVVVDFTTPDTCLSNARLCLEAGVHCVMGTTGADFSQLEGVGSANLFVAPNFAI
                     GAVLMMEASKLAAKHMPECEIVELHHDRKLDAPSGTAARTAELIREAGGNVHEPIHSI
                     RLPGLVAHQEVVFGGLGQTLSIRHDSIARESFMPGVLLAVRKVGTLERSPTFGLETLL
                     "
     gene            9254..10450
                     /locus_tag="VF517_03375"
     CDS             9254..10450
                     /locus_tag="VF517_03375"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX8052006.1"
                     /translation="MRLALALALLGALVLAPSAHAYKRLSYKFPSRTITYYDATGSRY
                     KNEVTAAAAAWNKSGARVHWVKASKSRARVPIRVNSKIGAAGLGWYFGNGRGLIELQP
                     TLKKGQLTKRAGEGVATQVIVHEMGHVMGLDHVTNVCAIMQPSLGSGCPQPAEVWRFR
                     CRLLEKDDIRGAVALFGGKIGKIGPEYCDRVAPPAAPSNLAVAPSSEEGTAQVTWTTP
                     KGSEIESVRVMRRRDVCPTGPDDPAADLVGQETANPGQAQSTIDYSGFPASGRYCYSV
                     QAFGALQRPGKVATVQYDYTGPVAPGAKPSADFNWQPEDGDGQTIAFFDGSEPADDDG
                     QIVAWRWDFADGQSSSERNPTHRFAPGSYSVTLTVTDDQGRTDFITRTVSVEEPIDGG
                     GEPEAP"
     gene            complement(10447..10944)
                     /locus_tag="VF517_03380"
     CDS             complement(10447..10944)
                     /locus_tag="VF517_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8052007.1"
                     /translation="MRLADGTRVVIRPIRPADRRLLEHSRDRFSDESMRRRFLAPKPR
                     LTSSELRYLTEVDGEDHYAVVAVPLDDLETIVGVGRFVRLADEPGAAEAAIIVADYVQ
                     GKGLGKRLAHELADAARDRGIRRFRASMLSDNRAALALMRTLSERLESHYDQGYREVV
                     SELAA"
     gene            11048..12145
                     /locus_tag="VF517_03385"
     CDS             11048..12145
                     /locus_tag="VF517_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8052008.1"
                     /translation="MPFLKLALVGVVILAAPLVPGLPDLDPAKPPPPQQAPPEQPPPP
                     RSESSAPPPSKEALRVCKRMAAPWGNDRASGKPREPFRSPGRLATSLRPGWTGCLRSG
                     TYSESEVVVKRRKITLRSSPGERATWRGRIVLQGRRQKLVDLTLDGTDGRKSLPNPTV
                     NAPEVTISDSDITNRKGICINVRSWRGPRPDGFVIQRNRIHDCGRRPPTNHDHGIYVV
                     DAVGGLIRDNVIFRNADRGVQLFPNAERVTVVRNTIDGNGSGVIFSNDSSNNLVRDNV
                     ISNSVERWNAESHKLRGAGNRFESNCLKPGNDEEGYNQNGGVMLPAIVAQSGNVVTDH
                     EPYRARSDGDYTPAGDACGKRGARGPARKPR"
     gene            12232..12558
                     /locus_tag="VF517_03390"
     CDS             12232..12558
                     /locus_tag="VF517_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052009.1"
                     /translation="MLTLESALATALIVSNTGSLSRGAKWIALLELGLSVAWLAVGYV
                     GRDRALAHRRDLEEAGRAWAAEAGLEPGYRPVGAGPKVVLVAVAGPALLIVGWAALFV
                     VLLLID"
     gene            complement(12574..13071)
                     /locus_tag="VF517_03395"
     CDS             complement(12574..13071)
                     /locus_tag="VF517_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX8052010.1"
                     /translation="MAHETEADLRELQRLLDECHAGAGAHLKRIFSDERRVNAADLPA
                     RLPGVQVTALATVTAKGEPRVAPVDGLFYRGRLWFGSAPDSQRFRNIRARPAVSATIA
                     HGEEFALVVHGTAREVDIGEPEHEGFRDYIGEVYGRDRIEWFSGHPFAWIEPTWMFTF
                     GGVSG"
     gene            complement(13170..13616)
                     /locus_tag="VF517_03400"
     CDS             complement(13170..13616)
                     /locus_tag="VF517_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HEX8052011.1"
                     /translation="MATSHVDYSHRSTVQKLGVREGDRVEVVGDVGSGLRRDVKEAAG
                     RGLVRSGDLDGAIVLVESLEEAERCLDSYRPRLRDDGYLWLITWKRGHDAYLNQIHLV
                     PPAKERSLIDNKTCSVDDERSGIRFVVPRALRKKPDANGGGNGNGA"
     gene            13707..14558
                     /gene="dapA"
                     /locus_tag="VF517_03405"
     CDS             13707..14558
                     /gene="dapA"
                     /locus_tag="VF517_03405"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX8052012.1"
                     /translation="MPIGGVLTAMVTPFGADGRVDEDAAAELIRHLLANGSDGIVVAG
                     TTGEGATLDDEEKCRLFELAVAESGDALVVAGTGSNDTAHTVHLTERATAIGVDAHLV
                     VTPYYNKPNARGIMAHFEAVAAATDLPIIAYNIPSRCVVDVPNELLREMAATIPNVQA
                     VKQANDDNLEAIEGLDLLAGNDDVLARTMDAGGTGGILVASHLFGREMKRIVEEPDAR
                     QEIHESLTDAFEALAVTTNPMPIKAALRMAGHSVGGFRLPMVELSDEEEAVVRAMLER
                     YQLLSAV"
     gene            14555..16240
                     /locus_tag="VF517_03410"
     CDS             14555..16240
                     /locus_tag="VF517_03410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX8052013.1"
                     /translation="MSGAPNGTLRVLPLGGLGEIGKNMTVVEYDGRIVVVDTGLMFPT
                     PDQLGVDLVLPDFAYLRERAADIEAIVLTHGHEDHVGALPYVLRELGPDHRPPVYGGP
                     LTVGLVRSKLDEHKLGERTKLIDVGPGETFDAGPFSIEPIRLSHSIPDSLGMALTCDL
                     GTTLITGDYKFDQTPVDGQPADMARLAELGREGLLLLCGDSTNVDRSGVALSEASIGP
                     HLVEAIARCKGRVVVTCFASNIHRVQQVVDAAGAVGRKVSLVGRSMRKNVNIGRGLGH
                     IHVPDGMLVGVREIEDFPDHKLVVISTGSQGEPLSALRRMAHGDHPQVELREGDTVIF
                     SATPIPGNERAVNETINRIYQLGADVITARDAPIHASGHGYAEELKLMINLTSPEYVM
                     PMHGDYRRMRLHRELAESVGVPRENAFVGANGLPLEIDKRGARLGKSEQAGMIFVDGV
                     DVGDVEDIALRDRRMLSADGIFIVVATVSEQDGRSVAPPELIFRGVPFLDSGDDDLLD
                     GLRMAVDESLARSAKEQISEIDLLQSHLHDDVARFVYEKIKRRPMVLPVVVEV"
     gene            complement(16197..17651)
                     /locus_tag="VF517_03415"
     CDS             complement(16197..17651)
                     /locus_tag="VF517_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8052014.1"
                     /translation="MNALRVLPIRYRLAAISAVLTFVILLLFSLMVGKFAGDQVRSSF
                     DDDLRRTAADLEERLPAKTDFQGSVFFPTAHDVYEAAASGDAEVRILSLSTGAVYLPR
                     GADPILGPPRTDIVDHGHFRVASRILRAPGGQPIAVLQYGKPRDHINATVTRIRVLLG
                     LGVIGGAALAFLAGLALARRAMAPIASLTQAAREIERTRDPAVELPKPEADDEVADLA
                     RTLEGMLRALDAARHETESALARQREFVADASHELRTPLTSILANLELLEAGLHGDDA
                     ETAAAALRSSRRMRRLVGDLLLLARADAGREGLRERVDLASVAAEAAAEASPVGADHR
                     VELALPPRGPVVFGVSDDLHRLMLNLIENALVHTPPGTEVTVRVGGAAGWATLEVEDS
                     GPGIPAQARERIFDRFVRGAGETGGGSGLGLAIVRAVAERHGGGVEVSEGTVGGARFT
                     VRLPEAAPPPPPDPGQRLGGLIPPPPRAAPSDAA"
     gene            complement(17648..18325)
                     /locus_tag="VF517_03420"
     CDS             complement(17648..18325)
                     /locus_tag="VF517_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8052015.1"
                     /translation="MSKPRVLVVEDDGDIADVLRRSLRLEGYDVRVAGDGVVALDEAS
                     VFEPDAVVLDLGLPRLDGVEVCRRLRADGDVPILMLTARDGVDARVAGLDSGADDYLV
                     KPFERQELLARLRALLRRRPPRGSAFLTAGDLRLNPDTREVSRGERMIDLTAREFELL
                     EYLMRNERLVVSREALLEEVWGYAPFSETNTVDVFVSNLRRKLEGGGEPRLLHTVRGA
                     GYVLRSS"
     gene            18431..>18861
                     /locus_tag="VF517_03425"
     CDS             18431..>18861
                     /locus_tag="VF517_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8052016.1"
                     /translation="MPATTTASTATRAEAFEHAWDDFFAASRRARGRAAQDRSGGLTP
                     AQYHLVAPLDGAAPLACGELALAAGVAAPTASRMLDALVRDGIVTREGSTADRRVVSV
                     ALTAKGRRLVRDKAKLISSKRRAVYASLTEDEREQAIVLLGR"
BASE COUNT         2851 a         6766 c         6662 g         2582 t
ORIGIN      
        1 gatctccgcc acgcgcgcgt ctacgtgagc gtgctgggca gcgaggtcga acgtgaggcg
       61 gccctcgatg ggctgacctc ggcgcgcggc ttcctccagt cgcaggtcgg caccgagctg
      121 cgcatgaagc gcacgccgac actggagttc gtgaacgacc cgtcgataga gcacgggatg
      181 aagatggagc gactgatctc ggaagtgacg gacgagggat gagcgacgac cgcacccagg
      241 tgctcgacga gctgcgcgcg gcggacaagc tcctcctgac gacccacgag aaccccgacg
      301 gcgacgcgct cggctccctg cgcgccacgc agatcgtgat ggaggcgctc ggcaaggact
      361 ccgagatgtt catggcggcg gacaacttcc cgctgccgca cgagtaccgc cacatgccgt
      421 tcgacgatgt cctgaacgag cccccggccg acctctccga gcggctggtc gtgttcctcg
      481 actgcggcaa catcgaccgc atgccgatcg acctcaccgg cggggtgcgg atcgtcaaca
      541 tcgaccacca ccacgacaac acgcgcttcg gcgacgtgaa ctacgtggtc gccgacgcgt
      601 cgtgcacggc tcagatcgtc cacgacctcg cccgcgagct cggggtgacg atcgacaagc
      661 cgctcgccga ggcgctctac atcgcgctcg tcaccgacac cggcaagttc atgttcgaga
      721 acacaactcc cgccgcacac cagatggctg cggacctgat cgccgccggc gtgcagcccc
      781 aggaggtcca ccagcgcctc tacgagggcc tgccgttcag ccggatccag ctcctcgcgc
      841 gcgcgctggc cgcggtgcag cgcttcgacg acggctcgat cacgctcacc cacctgacga
      901 tcgacgactt caaccactcg ggggcgaagg agtccgactc cgagggcatc gtcgaccaca
      961 tgcgccaggt cgagggcacg ctggtcgccg cgctggtgcg cgagctgctc gaggacaacg
     1021 gccgccgcaa ggtctcgctg cgcgccacca acggccgcgt ggacgtctcg aagatcgcgc
     1081 gggggttcgg cggcggcggt catcgccagg cggccggctt cacgaccgac gtcgagctgc
     1141 ccgagatgat cgagcggctg cggaccgagg tcaacgcgca gctctgaacg gcgtcgtcct
     1201 cttcgcgaag cccgcgggcg tgacctcgca cgacgtggtc gcccaggtgc gcaggtcgct
     1261 cggatgcaag gccgggcacg ccggcacgct cgatccgttc gccaccggcc tgctgctcgt
     1321 gctggtgggc cgcgcgaccc gcgtgcagcg gttcctgatg gcgctgccga agacctatcg
     1381 cgtcaccgcc cggctcggct ggacctccga cacaggggac cgcgacggcg cgctcgctca
     1441 tactggtcgc gtgcccgaga ccctctcgat cccggtcggc gagatcctcc agcgcccgcc
     1501 cgcgtactcc gccgtgaagg tcgggggcga gcggctctac gcgcgcgccc gccgcggcga
     1561 cccggtcgag ggcgagccgc gccccgtcac cgtgcaccgc gccgagctgc tctggcacga
     1621 gggcgagcgg gccggcttcg agatcgaatg ctcgtccggg acctatgtcc gccagctcgt
     1681 gaccgggctc ggcgacgcct actgcgagga gctcgagcgc accgcgatcg gcgggttccg
     1741 gctcgaggac gccgaccccg agcgggtggt gccgctcgag gaggcgctgg ggttcatgcc
     1801 cgaggcgccg ctcggcgagg aggacgcggc gaaggtgtcc cacggggtgg gggtgtccca
     1861 cggggtgggg gtgtccgcgc ccgcggacgg ctccgccggc cccgcctcct ccaccgaggc
     1921 cgtccgcctg acccacggcg gccgcctcct cgccatcggc cgtcccgagg gggacctgct
     1981 caagccgttc gtggtgttcc ccgaatgaag gtcacctcgc tcccggacgc cggccggcgc
     2041 cccgacgggc cacggcgcgt ggccgttggc accttcgacg gggtccacct cggtcaccgc
     2101 gccgtgatcg agggctgcga caccgtgctc acgttcgacc cgcacccgct cgcggtgatc
     2161 gcgccgcacg cgctgccgaa gttgctcgac tcgttcccga tcaagcgcga cctgatcgcc
     2221 ggcctcgggg tggaggagct agtcgtcatc ccgttcgacg ccgagttcgc cacccgcagc
     2281 gccgaggact tcatcgacaa cgagctggtc gagcggctcg gcgcggaggt cgtctgcgtc
     2341 ggggagaact tccgcttcgg caagggcgca cgcggggacg cggcgatgct cgacgcgcat
     2401 ccggccttcg agacgcgcgt cgtcccgatg gtcgaggccg cgggggagac cgtgtcctcg
     2461 acccacatcc gcgggctcgt gctcgctggc gaggttgccc gcgccgccga gttcctcgga
     2521 gcgccgttca tgatggaggg cgaggtcgtg cacggtgaca agcgcggtcg cgagctgggg
     2581 atgccgaccg ccaacctggt gccggacccg cggctcgccg tgcccggcca tggggtctac
     2641 gccgcgtggg cccacgggta cccggcggcc gtcaacgtcg gcatccgccc gaccttcgac
     2701 accggccgcg gcctgctgat cgaggcatac ctcctcgact tcgacggcga cctctacggg
     2761 cagacgctgc gcatcgcgtt cgcggagcgc ctgcgcggcg agaagcgctt cgactcggtc
     2821 gacgggctgg tcgagcagat gcagcgggac gtcgagcacg tccgtcgcgc tgctaacttt
     2881 gcgcgccgat gagcctgacc atcgacgcca agcgcgagat catccagaag cacggcaaga
     2941 ccgaggccga caccgggtcg cccgaggtcc agatcgccct gctcagcgcg cgaatcagcc
     3001 atctcaccga gcacctgcgc gagcacaagc acgaccacca ctcgcgccgc ggcctgctga
     3061 tgctcgtggg ccagcgccgg cgtctcctga actacctcca gcgcaaggac ctcgacaggt
     3121 accgcgcgct catcgccgag ctcggcctca ggcgatgatc gaggcgggca cgcccgcccc
     3181 ggatttcgcg ctgcccgacc aggacggcaa cgaggtcacg ctcgagtcgc tgcgcggcag
     3241 gcgcgtcgtg ctcgtcttct acccggccga cttcagcccg gtctgcaccg accagctcaa
     3301 cgtgtaccag gaggtgctcg gcgagctgaa ggagcggggc gccgagctgt acggcgtctc
     3361 ggtcgactcg aactggtgcc acagggcgtt ccgcgagcac ctcggcgtgg agatcccgct
     3421 gctggcggac ttccatccga aggcagctgt gtcgcaggcg tacggcgtgt acgccgagaa
     3481 gtacggcgtg tcgctgcgag cgctcgtgat gatcggtccc gacggcaccg tcgagtggac
     3541 gcacgtctcg ccgtcgccgc tggagatccc gggcgcgaac ctgatcttcg acgccctcga
     3601 ggcccacgcc gccgcctgac ctgacgtcgg ccgggccgcc gccgcccggc cccgaggacc
     3661 acatccgcgg cgacggtccc ctcgtcgtcg agtacggcga ctaccagtgc ccctactgcg
     3721 cgcaggccga tgcgctgctc gccgagctac ccgtccggcg cgtcttccgc cacttcccgg
     3781 tcgtctcgaa gcacccgcgg gcgcgcgtgc tggccgccgc cgccgaggcc gccgggatcc
     3841 agggcgcctt ctgggagttt cacgactcgc tctacgccga tcagggccgc ctcgacgacc
     3901 cgcacctctg gagccgggtg gagagcctcg gtctggacct cgaccgcttc gaggccgacc
     3961 gccgcgccga ggccgtggcc gagcgcgtcg ggcgcgactt caagtcggcg atccgcgcgg
     4021 gcgtgaccac cacgccgacg ctgttcgtgg acggcgtcgc ccaccccggc gtgcccgacc
     4081 cggagctgct ggcgcggctc cgcggctagc gcaagcttcg cgggctggcg acgaatgccg
     4141 agctgacgca agcggcgtta ctcgcgccgg tgtgtgagcg gattgggcgc cgtgccagtg
     4201 gcatcggcgt gcagggtctc cggtgccaca tccgccccgt tcggccagac gatcgtgccg
     4261 agtccctcgt cgagttcgac ccgagcgaag tactcgggat cctctagtgg tgcgaagacc
     4321 ccatgccagc tccaatgaga cgcgtcgatc acgccctcgg cgccgtcgtc gaacgcgagg
     4381 cggaggcggt ggtttccgac cagttcgacg gccgtcacgt gtcgtagggc ttcaggcact
     4441 cggaccattt taagcaagcg ggtcgatgcg cacgagcggc tgcccgcgcc tcgcgcgctc
     4501 ccagttgtgg agcagttcac gacgccgtag gcgtccccag tcgcggacca tccgcatggc
     4561 gcgtggcgga agctcgccgg cgagggggcg gagatcatca atcgcgaacg tcgcccacac
     4621 acctgcgtac gaagcgtgaa agtgcggcac gccgtgttcc tcgccgaagt acatcgctat
     4681 gacgattccg tagaactcgc agattcgcgg catccctcgg tacagccacg ggaccgcgtt
     4741 tctccgcccc cgccgcctcc gcacccactg ctagcctctc ccgaacttca tttcgaagcg
     4801 attaggcaat gagccgccaa ccggaggcgg cgagagagga atcctcatga atcaggacgt
     4861 aacccgggtc tccgtagaga tcggcgggac cgaactttcg ttcgagaccg ggaagctcgc
     4921 caagcaggcg agcgggtccg tagtagtcca ggccggcgac accatggtgc tcggcaccgc
     4981 caccgccggc aacgagcgcg atgtcgactt tctgccgctg acggtggacg tcgaggagcg
     5041 catgtacgcc gcgggcaaga tccccggcag cttcttcaag cgcgagggcc gtgccggcga
     5101 gaagggcacc ctcaccgcgc gcatgatcga ccgcccgctg cgcccgctct tcccgaaggg
     5161 ctggcaccgc gagacgcagc tcgtctcgat cccgatgtcg gtggaccacg tccaccccta
     5221 cgacatcctc gcgatgaacg gcgcgagcgc cgctctgatc atctccgaca tccccttcga
     5281 cacccccgtc ggcgccgtcc gcatcggcca ggacgaggag ggtgggttca tcgtgaaccc
     5341 ggacgaggag tggctgctcg agacgccgct cgacctcatc gtcgccggca ccgaggaggc
     5401 catcctcatg gtggaggccg gcgccaagga ggtctccgag gccgtcatcc tcgacgcgct
     5461 cgacatcgcc cacggcgaga tcaagaagct ctgcgccctc cagcgcgaac tccagcagaa
     5521 ggtcggcaag gagaagatcg tcctcgaggc ccccaaggtc gacgagtctg tcctcagcca
     5581 gctcaaggac cagttcggcg cgaagctcga cgaggccacc caggtcacgg acaagctcga
     5641 gcgccaggac gcgaccaagg ccgtcgagac cgaggccgtc gaggcgctcg cgggcgaccc
     5701 cgacgcggag gaccacgccc agaagcgcgc cgccgcccag ctcgcgttcg acaagctcga
     5761 gaaggaagtg atccgcaggc ggatcgccgt cgacaagaag cgccccgacg ggcgttcggc
     5821 ggaggagatc cgcgacatct ggatcgaggt gggcgtctcg ccgcgtgcgc acggctcggc
     5881 cgtcttcacc cgcggtcaga cccaggcgtt cagcgtcgcg gccctcggca ccacgcgcga
     5941 ggagatgcgg ctcgacacgc tcggcctcga gaccacgaag cactacttcc accactacaa
     6001 cttcccgccg ttctcggtgg gcgaggccgg cttcatgcgc ggtccgaagc gccgcgacat
     6061 cggccacggc gcgctcgctg agcgggcgct cgtgccggtc gtcccgagca cggaggactt
     6121 cccgtacacg atccgcgtgg tctcggacat cctcgagtcg aacggctcgt cgtcgatggc
     6181 ctcggtctgc ggctcgagcc tctcgctgat gcaggcgggc gtgcccctca aggcgccggt
     6241 cgccggtatc gcgatgggcc tgatcaagga gggcgacgac tacgtcgtgc tctccgacat
     6301 cgcgggcgtg gaggaccacc tcggcgacat ggacttcaag gtcgccggca ccgagcaggg
     6361 catcaccgcc cttcagatgg acatcaagat cacgggcgtc acgttcgaga tcctgagcga
     6421 tgcgctcgcg caggccaagc agggccgcac gcacatcctc ggcaagatga acgacgtcat
     6481 ctcggagccc agctccgagc tctccccgca cgcgccgcgg atcacctcga tccagatcga
     6541 caccgacaag atcgggatgg tcatcggcaa gggcggcgag acgatccgcg cgctgtcgga
     6601 ggagttcgac gcgcagatcg acatgcagga cgacggcatg gtcctcgtct acgcctcgaa
     6661 cggcgagcag ggcgacgccc tcgtcgagcg gatccgctcg atgaccaagg acgtcgaggt
     6721 cggcgacgag ttcaccggca aggtcgtgaa gacgaccacg ttcggcgcgt tcgtcgagct
     6781 ctcgaagggc accgacggcc tgctgcacat ctcgaacctg aagccgggtg agcgcgtcga
     6841 gtccgtcgag gacgtggtca accgcggcga cgaggtgagc gtcaaggtgg tcgaggtcga
     6901 ccgcgagcgc ggccgcatcg gtctgcggat ggcggacgac ccagacgttg ccggcaagtc
     6961 gcccgaggag ctggccaacg tcggcaccgg tggtggcggc ggaggcggcg gaggcgggcg
     7021 tgaccgcgat cgcggcgggc gcggcggcgg cgaccgcggt ggccgtggtg gtggccgcgg
     7081 gcgcgacggc gaccgtggcg gccggggtag ccgcggcggc gaccgtggcg agcgcggcgg
     7141 cggcgaccgt cccaactacg cgcgcgacga gcgctagagc actgaccacc accgacggtc
     7201 acagcctcac tcggctcgac tcgggagtcc gggtcgtgtc cgagcggctg ccgtcggtgc
     7261 ggtcgatcgc gctcggcctc ttcgtcggca cgggctcgag gaacgagacc cccgagcagg
     7321 gcgggatctc gcacttcctc gagcacttgc tgttcaaggg gaccgaccgg ttcagctcga
     7381 tcgagatcga ccagacgttc gacggcatgg gtgccgaggc caacgccggc accggcaagg
     7441 agaccacctc ggtctactcg cgcttcctcg accagcacct cgagcgcgcg tacgacgtgc
     7501 ttgcggacat ggtcctgcgg ccggcgtacc cggacatcga ctccgagcgc caggtggtga
     7561 tcgaggagat cgcgatgtac gaggacgagc cggcggacaa ggtccacgac gtgctggcgc
     7621 aggcgctgtt cggcgaccat ccgctcgggc gcccgatcat cggcaccgcc gacgtgatcg
     7681 cgaacgtgcc ggtgcccgac atcgcgagct accacgacag ccgctacgtc ggcccgaacc
     7741 tcgtggtctc cgccgcgggg aacgtggacc acggccgcct ggtcgagctc accgagcgct
     7801 tcttcacggc gcccacgggc gacgccgacg gccccaccgc cgcacccgcc agctgcccgc
     7861 cccgcgccgc cttccacgag aagacgaccg agcagtacca cctctgcctg ggcggccccg
     7921 gcatcgcgcg ccacgacgat cgccgcttcg ccctgcgcgt gctcgacgcg atcctcggcg
     7981 ggtcgtcctc gtcgcgactg ttccaggagg tgcgcgagaa gcgcggcctc gcctactcgg
     8041 tctactcgta cgcgagccac ttcgccgacg cgggccaggt cggcgtctat gtcggcaccc
     8101 gcccggacaa cgtgcgtgag gcgatggacg tgatcggcac cgagctgcgc cggatcgtgg
     8161 aggagcccgt ggcgggcgac gagctcgagc gcgcgaagga gaacgtgacg ggccggatgg
     8221 tgctctcgtt cgagtccacg ctcacgcgca tgaaccgcct cggcggctcc gtgctgatgg
     8281 acgtgccgct gctctcgctc gacgagatga tcgccgcggt ggacgccgtc gacctcgacg
     8341 ccgtgtcgga gctctcacgc gagctgttcg cgcccgaaaa gctgtcggcg gccggagtgg
     8401 gggggagcga ggacgcgttc cgcgcggcgc tcgagccggt cagcggcgag ctggcggccg
     8461 ctgcgtgatc aacgtcgccg tgtccggtgc ggccggtcgg atgggccagg cagtggtcgc
     8521 ggcggtcgag ggcgccgacg acatggcgct ggtgggcaag gccgacccgc tgttcgagca
     8581 gtcgctccag gacgtgctcg gcgacgccga cgtggtggtc gacttcacga cccccgacac
     8641 ctgcctctcg aacgcgcggc tgtgcctgga ggcgggcgtg cactgcgtga tgggcaccac
     8701 cggcgccgac ttctctcagc tcgagggggt cggcagcgcg aacctgttcg tggcgccgaa
     8761 cttcgcgatc ggcgcggtgc tgatgatgga ggcctcgaag ctcgcggcca agcacatgcc
     8821 cgagtgcgag atcgtcgagc tccaccacga ccgcaagctc gacgcgccca gcggcaccgc
     8881 cgcccgcacg gcggagctga tccgcgaggc cggcggcaac gtgcacgagc cgatccactc
     8941 gatccgcctg ccgggtctcg tcgcccacca ggaggtcgtc ttcggcgggc tgggccagac
     9001 gctctccatc aggcacgact cgatcgcccg cgagtcgttc atgcccggcg tcctgctggc
     9061 cgtgcgcaag gtcggcaccc tggagcgctc gcccacgttc gggctcgaga cgctgctcta
     9121 gcgcgcgcgg ccacgccccc ctccgttgga gccggagcac gggagcccgg cgtgcggcga
     9181 ccctaagcgg gccgggccgg cgccgtcaag cgtggggctg gcggttctcg gcttgcgcgc
     9241 ttaccctcga cacgtgcgcc tcgctctcgc cctcgcgctc cttggcgcgc tcgtactcgc
     9301 gccctcggca catgcctaca agcggctgag ctacaagttc ccgtcgcgca cgatcacgta
     9361 ctacgacgcg accggctcgc gctacaagaa cgaggtcacc gccgctgccg ccgcctggaa
     9421 caagagcggg gccagggtcc actgggtgaa ggcctcgaag agccgcgcgc gggtgccgat
     9481 ccgggtcaac tcgaagatcg gcgccgccgg gctcggctgg tacttcggca acgggcgcgg
     9541 gctcatcgag ctccagccga ccctcaagaa gggccagctc acgaagcgcg ccggtgaggg
     9601 tgtcgcgacc caggtgatcg tccacgagat gggccacgtc atgggcctcg accacgtgac
     9661 gaacgtctgc gcgatcatgc agccgtcgct cggctcgggc tgtccccagc cggccgaggt
     9721 ctggcgcttc cgctgccgcc tgctcgagaa ggacgacatc cgcggagccg ttgccctgtt
     9781 cggcgggaag atcggaaaga tcgggccgga gtactgcgac cgggtggccc cgccggcggc
     9841 gccctccaac ctggcggtgg cgccgagctc ggaggagggg accgcacagg tcacctggac
     9901 taccccgaag gggtccgaga tcgagtccgt gcgcgtgatg cgccgccgcg acgtgtgccc
     9961 gaccgggccc gacgaccctg cggctgatct cgtcggccag gagaccgcga atccgggcca
    10021 ggcgcagtcg acgatcgact acagcgggtt cccggcgagc gggcgctact gctactcggt
    10081 gcaggccttc ggcgcgctgc agcgcccggg caaggtcgcc acggtccagt acgactacac
    10141 cgggcccgtg gcccccggag ccaagccgtc ggccgacttc aactggcagc cggaggacgg
    10201 cgacggccag acgatcgcct tcttcgacgg ctcggagccg gcggacgacg acggtcagat
    10261 cgtcgcgtgg cgctgggact tcgccgacgg ccagtcgtcg agcgagcgca acccgacgca
    10321 caggttcgcg cccggcagct acagcgtgac gctgaccgtg accgacgatc aggggcgcac
    10381 ggacttcatc acccggaccg tctcggtgga agagccgatc gacggcggcg gcgagcccga
    10441 ggctccctag gccgcgagct ccgacacgac ctcgcggtac ccctgatcgt aatgcgactc
    10501 gagccgctcg gagagcgttc gcatgagcgc gagcgcggcc cggttgtcgg agagcatcga
    10561 ggcgcggaag cggcggatgc cgcggtcgcg ggccgcgtcg gccagctcgt gggccagccg
    10621 cttgccgagg cccttgccct ggacatagtc ggccacgatg atcgcggcct cggcggcgcc
    10681 gggctcgtcg gccagacgca cgaagcgccc gacgccgacg atcgtttcga ggtcgtcgag
    10741 cgggaccgcc accacggcgt agtggtcctc gccgtcgacc tcggtgaggt agcgcagctc
    10801 gctcgaggtc aggcgcggct tgggggcgag gaagcgccgg cgcatgctct cgtcggagaa
    10861 acggtcgcgg ctgtgctcga gcaaccggcg gtcggccggg cggatggggc ggatgaccac
    10921 ccgggtgccg tctgcgagcc tcacgaccat agttcgaccg tagtcgaact gccggtggcg
    10981 gtgtgagtgc ttggtcacgc cgggctaccc ttggcgcgat gacagggacg tcatcaggag
    11041 gaggcccatg ccgttcttga agcttgcatt ggtgggagtg gtgatcctcg cggcgccgct
    11101 cgtccccggg ctccccgacc tcgacccggc caagccgccg ccgccgcaac aggcgccgcc
    11161 ggagcagccg cctccgccgc gatcggagtc ctcggccccg ccgccgtcga aggaggcgct
    11221 gcgcgtgtgc aagcgcatgg cggctccatg gggcaacgac cgggcctccg gcaagccacg
    11281 cgagccgttc cggagcccgg ggcggctcgc cacgtcgctg cggccggggt ggacgggctg
    11341 cctgcgctcc ggcacctaca gtgagtccga ggtggtcgtg aagcgccgca agatcacgct
    11401 gcgcagctcg cccggcgagc gcgccacctg gcgcgggcgg atcgtgctcc aggggaggcg
    11461 tcagaagctc gtggacctga cgctcgacgg caccgacggg cgcaagtcgc tcccgaaccc
    11521 gacggtgaac gcccccgagg tgacgatctc cgacagcgac atcacaaacc gcaagggcat
    11581 ctgcatcaac gtgcggagct ggcgcggccc gcggcccgac ggcttcgtga tccagcgcaa
    11641 ccgcatccac gactgcggcc ggcgcccgcc caccaaccac gaccacggca tctacgtggt
    11701 cgacgccgtc ggcgggctga tccgcgacaa cgtgatcttc cgcaacgccg accgcggcgt
    11761 gcagctcttc ccgaacgccg agcgcgtcac cgtcgtgcgc aacacgatcg acggcaacgg
    11821 aagcggcgtg atcttctcca acgacagctc gaacaacctc gtgcgcgaca acgtgatctc
    11881 gaactcggtg gagcgctgga acgcggagag ccacaagctg cgcggcgcgg gcaaccgctt
    11941 cgagtcgaac tgcctgaagc ccgggaacga cgaggagggc tacaaccaga acggcggcgt
    12001 gatgctgccg gcgatcgtgg cgcagtccgg gaacgtcgtg accgaccacg agccctaccg
    12061 cgcccgctcg gacggcgact acacgcccgc cggcgacgcc tgcggcaagc gcggtgcccg
    12121 cgggcccgcg cgcaagccgc gctgatgccg aacgcgctcc tcgaggacta caagctgaag
    12181 gtggccttcg ccaccgagca ggtcaatcgc ctccagaccc agttctaggt gatgctcacc
    12241 ctcgaatccg ctctcgccac tgctctgatc gtctcgaaca cggggtcgtt gagcaggggt
    12301 gcgaagtgga ttgcgctgct ggagctgggc ctctcggtgg catggctggc ggtcggctac
    12361 gtcggccgcg accgagccct ggcacacagg cgcgacctcg aggaggcggg gcgtgcttgg
    12421 gccgcggagg cggggctcga gcccggctac cgcccggtgg gagccgggcc gaaggtcgtc
    12481 ctggtcgccg ttgccggtcc cgccctgctc atcgtcgggt gggcggcgct gttcgtagtg
    12541 ctgcttctga tcgactgaga ggcgcccgcg gcctcagccg ctcacgccgc cgaacgtgaa
    12601 catccacgtc ggctcgatcc acgcgaacgg gtggccgctg aaccactcga tccgatcgcg
    12661 gccgtagacc tcgcctatgt aatcgcggaa gccctcgtgc tcgggctcgc cgatgtcgac
    12721 ctcgcgggcg gtgccgtgca ccaccagcgc gaactcctcg ccgtgcgcga tcgtcgcgct
    12781 caccgccggt cgcgcgcgga tgttgcggaa gcgctgcgag tccggcgccg agccgaacca
    12841 cagccggccg cggtagaaga ggccgtcgac cggcgccacg cgcggctcgc ccttcgcggt
    12901 cacggtcgcg agcgccgtga cctgcacgcc tggcagccgc gccggcaggt ccgcggcgtt
    12961 cacccgccgc tcgtcgctga agatccgctt gaggtgcgcg cccgcgccgg catggcactc
    13021 gtccagcagc cgctggagct cgcggaggtc cgcttcggtc tcgtgcgcca tcgaaacatc
    13081 ctcgcgattc gcgcgactgg tcgactttgt gcccgctata ggtgcattag atcgaccagg
    13141 tgcagcggcc gcgcgctgca gcccggtggc tacgcgccgt tgccgttgcc gccgccgttg
    13201 gcgtcgggct tcttgcgcag cgcgcgcggg accacgaagc ggatcccaga gcgctcgtcg
    13261 tcgaccgagc aggtcttgtt gtcgatcagg ctgcgttcct tggcgggcgg gaccaggtgg
    13321 atctggttga gataggcgtc gtgcccgcgc ttccaggtga tcagccagag gtaaccgtcg
    13381 tcgcgcaggc gcgggcggta gctgtcgagg cagcgctcgg cctcctcgag cgactcgacc
    13441 aggacgatgg cgccgtccag gtcgccggag cgcacgagcc cgcgcccggc ggcctccttc
    13501 acgtcgcggc gcaggccgga accgacgtcg ccgaccacct cgacgcgatc gccctcccgc
    13561 accccgagct tctgcacggt ggagcggtgg ctgtaatcga cgtgggacgt ggccatttgc
    13621 aggcgtgatt gtaggaggcc gccaaaaggc ccctggcggc cgccgccccg acgattaccc
    13681 gctggcgcgc gcgataatct cgtcggatgc cgatcggggg cgtcctcacc gcgatggtca
    13741 cgccgttcgg cgcggacggg cgcgtggacg aggacgccgc ggccgagctg atccgccatc
    13801 tgctggccaa cggatccgac gggatcgtcg tcgccgggac caccggcgag ggcgcgacgc
    13861 tggacgacga ggagaagtgc cgcctgttcg agctggccgt cgccgagtcc ggcgacgccc
    13921 tggttgtggc cggcacgggc tcgaacgaca ccgcccacac ggtgcacctc accgagcgcg
    13981 cgaccgcgat cggcgtggac gcccacctcg tggtcacgcc ctactacaac aagccgaacg
    14041 ctcgcgggat catggcccac ttcgaggcgg tcgccgcggc caccgacctc ccgatcatcg
    14101 cctacaacat cccctcgcgc tgcgtggtgg acgtcccgaa cgagctgctg cgcgagatgg
    14161 ccgccacgat ccccaacgtc caggccgtca agcaggccaa cgacgacaac ctcgaggcga
    14221 tcgaggggct cgacctgctg gccggcaacg acgacgtgct cgcgcgcacg atggacgccg
    14281 gcggcaccgg cgggatcctc gtcgcctccc acctgttcgg cagggagatg aagcggatcg
    14341 tggaggagcc cgacgcccgc caggagatcc acgagtcgct cacggacgcg ttcgaggcgc
    14401 tggcggtcac gaccaacccg atgccgatca aggcggccct gcgcatggcg ggccacagcg
    14461 tcggcggctt ccgcctgccg atggtggagc tctccgacga ggaggaggcg gtggtccgcg
    14521 cgatgctcga gcgctaccag ctcctcagcg cggtttgagc ggcgccccga acggcaccct
    14581 ccgcgtgctc ccgctcgggg ggctggggga gatcggcaag aacatgacgg tggtcgagta
    14641 cgacggccgg atcgtcgtgg tcgacaccgg gctgatgttc ccgacacccg accagctcgg
    14701 cgtcgacctc gtcctgcccg acttcgccta cctgcgcgag cgcgccgccg acatcgaggc
    14761 gatcgtgctc acccacggcc acgaggacca cgttggcgcg ctgccgtacg tgctgcgcga
    14821 gctcggcccg gaccaccgtc cgccggtcta cggcgggccg ctgaccgtgg ggctggtgcg
    14881 ctcgaagctc gacgagcaca agctcgggga gcggaccaag ctgatcgacg tgggaccggg
    14941 ggagacgttc gacgccggcc cgttcagcat cgagccgatc cgcctctcgc actcgatccc
    15001 ggactcgctc ggcatggcgc tcacgtgcga cctgggcacc acgctcatca ccggcgacta
    15061 caagttcgac cagacgccgg tcgacggcca gccggccgac atggcgcggc tcgcggagct
    15121 cggccgcgag ggcctcctgc tgctctgtgg cgactcgacc aacgtcgacc gctccggcgt
    15181 ggcgctatcg gaggcgagca tcggcccgca cctggtcgag gcgatcgcgc gctgcaaggg
    15241 ccgggtggtg gtcacctgct tcgcgtcgaa catccaccgc gtgcagcagg tcgtggacgc
    15301 cgccggcgcc gtcgggcgga aggtctcgct cgtcggccgc tcgatgcgca agaacgtgaa
    15361 catcggccgc ggcctcggcc acatccacgt tcccgacggc atgctcgtgg gcgtgcgcga
    15421 gatcgaggac ttcccggacc acaagctcgt ggtgatctcg accggcagcc agggcgagcc
    15481 gctctcggcg ctgcgccgga tggcccacgg cgaccacccg caggtcgagc tgcgcgaggg
    15541 cgacacggtg atcttctcgg ccaccccgat ccccgggaac gagcgcgccg tgaacgagac
    15601 gatcaaccgg atctaccagc tcggcgccga cgtgatcacc gcgcgcgacg cgccgatcca
    15661 cgcctcgggc cacggctacg ccgaggaact gaagctgatg atcaacctga cctcgccgga
    15721 gtacgtgatg ccgatgcacg gcgactaccg ccggatgcgg ctgcaccgcg agctggccga
    15781 gagcgtgggc gtgccgcgcg agaacgcgtt cgtcggggcc aacgggctgc cgctcgagat
    15841 cgacaagcgc ggggcgcggc tcggcaagag cgagcaggcg gggatgatct tcgtggacgg
    15901 cgtcgacgtc ggcgacgtgg aggacatcgc gctgcgcgac cgccggatgc tgtccgcgga
    15961 cggcatcttc atcgtcgtgg ccacggtgtc cgagcaggac ggccgctcgg tggcaccgcc
    16021 ggagctgatc ttccgcgggg tcccgttcct cgacagcggc gacgacgacc tgctcgacgg
    16081 cctgcgcatg gcggtcgacg agtcgctcgc gcgctccgcc aaggagcaga tcagcgagat
    16141 cgacctcctc cagagccacc tgcacgacga cgtcgcgcgg ttcgtctacg agaagatcaa
    16201 gcggcgtccg atggtgctgc ccgtggtggt ggaggtatga ggccgccgag gcgctgaccg
    16261 gggtccggcg gcggaggcgg cgcggcctcg ggcaaccgga ccgtgaagcg ggcgccgccg
    16321 acggtcccct cggagacctc cacgccgccg ccgtggcgct cggccacagc gcggacgatc
    16381 gccagtccca ggccgctgcc gccgccggtc tcgccggcgc cgcgcacgaa gcggtcgaag
    16441 atccgctcgc gcgcctgcgc gggaataccg gggcccgagt cctccacctc gagcgtggcc
    16501 cagccggccg cgccgcccac gcgcacggtg acctccgtgc cgggcggggt gtggacgagc
    16561 gcgttctcga tcaggttcag catgagccgg tggaggtcgt cggagacgcc gaacacgacc
    16621 gggccgcgcg gcgggagggc gagctcgacc cggtggtcgg cgccgacggg ggaggcctcc
    16681 gccgccgcct cggcggcaac cgacgccagg tccacccgct cgcgcagtcc ctcgcggccg
    16741 gcgtccgcgc gggcgagcag cagcaggtcg cccaccagcc ggcgcatccg ccgcgaggag
    16801 cgcagcgccg ctgcggcggt ctcggcgtcg tcgccgtgga ggcccgcctc gagcagctcg
    16861 aggttcgcca ggatgctggt gagcggggtg cgcagctcgt gcgaggcgtc ggccacgaac
    16921 tcgcgctggc gggcgagggc gctctcggtc tcgtgccggg ccgcgtcgag cgccctgagc
    16981 atcccctcga gcgtgcgcgc gaggtcggcc acctcgtcgt cggcctcggg cttcggcagc
    17041 tcgacggccg ggtcgcgcgt gcgctcgatc tcgcgggcgg cctgggtgag gctggcgatc
    17101 ggggccatcg cgcggcgggc cagcgcgagc cccgccagga acgcgagcgc ggcgccgccg
    17161 atcacgccga ggccgagcag cacgcggatg cgcgtgaccg tcgcgttgat gtggtcgcgt
    17221 ggcttgccgt actggagcac cgcgatcggc tgcccgcctg gggcgcggag gatccgcgag
    17281 gccacgcgga agtgcccgtg gtccacgatg tcggtccgcg gcgggccgag gatcgggtcc
    17341 gcgccccgcg ggaggtagac cgcgccggtg ctcaggctga gaatgcgcac ctcggcgtcg
    17401 ccggacgccg ccgcctcgta gacgtcgtgc gcggtcggga agaacaccga gccctggaag
    17461 tcggtcttcg ccggcaggcg ctcctccagg tccgccgccg tccggcgcag gtcgtcgtcg
    17521 aacgacgagc gcacctggtc gcccgcgaac ttgccgacca tcagcgagaa cagcagcagg
    17581 atcacgaacg tgagcaccgc cgagatcgcg gccagccgat accggatcgg caggacgcgg
    17641 agggcgttca cgaggacctg agcacgtagc ccgcgccgcg cacggtgtgg agcagccgcg
    17701 gctcgccgcc gccctcgagc ttgcggcgca ggttcgagac gaacacgtcc accgtgttcg
    17761 tctcggagaa cggggcgtag ccccagacct cctcgagcag cgcctcgcgc gagacgacca
    17821 ggcgctcgtt gcgcatcagg tactcgagca gctcgaactc gcgcgcggtg aggtcgatca
    17881 tgcgctcgcc gcgtgagacc tcgcgcgtgt ccgggttcag ccgcaggtcg cccgcggtca
    17941 ggaacgcgct tccgcgcggt gggcggcggc gcagcagggc gcgcaggcgg gcgagcagct
    18001 cctggcgctc gaacggcttg acgaggtagt cgtcggcgcc ggagtcgagc ccggccacgc
    18061 gcgcgtcgac gccgtcgcgc gcggtgagca tcaggatcgg cacgtcgccg tcggcgcgca
    18121 gccgccggca gacctccacg ccgtcgaggc gggggagccc caggtcgagc acgaccgcgt
    18181 ccggctcgaa cacggacgcc tcgtcgagcg cgacgacgcc gtcgccggcc acgcgcacgt
    18241 cgtagccctc caggcgcagc gagcggcgca gcacgtcggc gatgtcgccg tcgtcctcca
    18301 caaccagcac gcggggcttg ctcatgccgt cgatcttagg cgccgaaagt cccgctcccg
    18361 gcaagattta gcgacttatt aggcgcgcgt ccgcattcgg tagcaagcta accattgcta
    18421 agctaatcaa atgcccgcca ccaccaccgc ctcgaccgcg acccgagccg aagccttcga
    18481 gcacgcctgg gacgacttct tcgcagccag ccgccgtgcg cgcggccgcg ccgcgcagga
    18541 ccgctccggc gggctcacgc ctgcgcagta ccacctcgtg gctccgctcg acggcgccgc
    18601 accgctcgcg tgcggcgagc tggcgctggc cgccggggtc gcggccccca ccgcgagccg
    18661 gatgctcgac gcgctggtgc gcgacgggat cgtgacgcgc gagggctcga ccgccgaccg
    18721 ccgcgtggtc tcggtcgcgc tgaccgcgaa gggccggcgg ctcgtgcgcg acaaggccaa
    18781 gctgatctcg tccaagcgcc gcgccgtgta cgcgagcctc accgaggacg agcgcgagca
    18841 ggcgatcgtg ctgctgggcc g
//
LOCUS       DASVPL010000072         7603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_283443, whole genome shotgun sequence.
ACCESSION   DASVPL010000072 DASVPL010000000
VERSION     DASVPL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283443"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5112)
                     /locus_tag="VF517_03430"
     CDS             complement(<1..5112)
                     /locus_tag="VF517_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX8052017.1"
                     /translation="MTESSAIAVVGLACRLPAAPDPDAFWQLLREGRDAIGDAPADRW
                     DTDALAGAEKSMPGLLRGGFLDRVDEFDAEFFGISPREAAVMDPQQRLILELSWEALE
                     DAGIVPDDIDGSRTGTFVGAISGDYGELLRRHGGEAITRHALTGTNRGIIANRVSYIL
                     GLRGPSLTVDAAQSSALVAVQLACESLLRGESTLALAGGVNLNISIDSAISAHRLGAL
                     SPDGRCFTFDSRANGYVRGEGGGVVALKPLESALAEGDPIHCVIRGAAVNNDGGGDGL
                     TAPDRDAQEEVLRLACERAGVESGDVQYVELHGTGTPLGDQVEAAALGAVLGAGRDGE
                     RPLVVGSAKTNVGHLEGAAGIVGLIKAILCIEHRELPGSLNFSEPAPGIPLEELHLRV
                     QRSLDSWPAPDRPLLAGVSSFGMGGTNCHVVLAEPPERGDAGAAAEEPAAGGPEVVPW
                     VLSGTSEGAVRDQARNLLAHVGDRPDLGAGDVGYSLVTTRSAFGHRAVVLGGDRERLL
                     EGLGSLSQGEPAAAVVAGEASSRGGGTVFVFPGQGSQWRGMALGLLDSAPVFAESMRA
                     CAEALAPHVDWSLEDVLRGVDGAASFERVDVVQPALFAVMVSLAALWRSYGVEPAAVV
                     GHSQGEIAAAHVAGALSLEDAARVVALRSQAVADDLAGHGGMVSVALAPGGAEELIGR
                     WGERVSIAAVNGPASVVVSGEMEALDELLAACEADGTWARKIPVDYPSHSVRVEQIRE
                     RMARDLAPIEPRSGTVPFFSTVEAEAIDTAGLDADYWYRNLRQRVRFYDVVRALIEEG
                     VGAFLEISPHPVMTAGAQETVEEMLGDDAADVAVLGTLRRDHGDLDRFFTSLAALYVR
                     GGAVDWRTVFAGSGARRVDLPTYAFQRRRHWLEGLGSSSAGAVSALAGAPGAEVDGDG
                     GSDAARPAGALAQRLDGLPKHDQVRTVLDVVLAQAAVVLGHDSAAAVDSKRAFKDLGF
                     DSAGAVELRNGLTAATGLRLPSSLLFDHPTPVAVADFLLAEATGTGRKASAVARASNA
                     EEPIAIVGMSCRYPGGVASAEALWELVASGRDAIGEFPDDRGWALDALYDPDPDSSGT
                     SYTRHGGFLYDAGEFDPDVFSISPREALAMDPQQRLLLEGAWEAFEDAGIAPSSLRGT
                     QTGVFVGVMASDYGPRLHEASGSTEGYALTGSLTSVASGRLAYMFGLEGPAVSVDTAC
                     SASLVALHLACQALRQGECELAVAGGATVMANPGIFVEFSRQRGLAVDGRCKAYGADA
                     DGTGWSEGAGLLVLEPLSRAQANGHEVLALVRGSAVNQDGASNGLTAPNGPSQERVIR
                     QALANAGLSPSDVDAVEGHGTGTTLGDPIEAQALLATYGADRSNGPLYLGSLKSNTGH
                     TQAAAGVGGVIKMVEALRHGLLPKTLHAGEPTPHVDWSAGAVELLQEPVEWPAGERVR
                     RAGVSSFGVSGTNAHVILEEPPVLTPRRVNGDGPVLEGSGANGGAPDLQAQAVNGDGS
                     ALNGDAPHGGVGDAEGGEVAEAGDAAEGDAAEGDAGAADGDAAAADGDAAASAARALP
                     FLVSAATPQALGEQAGRLAGFLERRPELDAYAVAAALATRRAQLPQRAVAVAADHAEL
                     VASLRALERGELANGVVRGSAAGGGATAFLFSGQGSQWAGMGSVLHREFPVFAQALDE
                     VCAELDRHLDRPLLEVMFAAEDSEEAELLADTRFTQ"
     gene            complement(5375..6571)
                     /locus_tag="VF517_03435"
     CDS             complement(5375..6571)
                     /locus_tag="VF517_03435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8052018.1"
                     /translation="MLDEGIQSGGGSAVLEEILPQVREFAKTELLPQQAHFDTLPDVP
                     VPANEAFHSLGLSGWWLPEEYGGRGLGLEESVDIVSELAYGDAGVAFTQFISILGTTT
                     VQLYGSDEVKERYLTTIPRDGGFCATLGSERAAGSELAKIVTTAARAGEDVVINGEKF
                     FSTNAGFAGFLIVIARSAANPEEHMAVVVPGDSQGVRMVKRWEMIGLRASGTYQVSFE
                     RCRVPSQGVLQGPGLRLLEIGLNASRILIASTALGISRRVRDLCMEYARRKRLGNSPL
                     TGNAVFAAKLGQMEMEIDAMRHVCRAAAREFDAIMQRDDAAEHFLRKGTLKSALTAKA
                     FCGQTGWKIAGVGSEMFGGLGYTREMVIDKLVRDIRHVSIIEGGDDVIRDLVYNRYVV
                     PEDNRV"
     gene            complement(6664..>7603)
                     /locus_tag="VF517_03440"
     CDS             complement(6664..>7603)
                     /locus_tag="VF517_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052019.1"
                     /translation="VLWDLAAETGMTIFGGGAAFFAGGMKEGVEPAAGRDLSALHAVG
                     STGSPLSPEAFAWIYDKLGSDTWLFSTSGGTDVCTAFVGGVPTLPVHRGELQAASLGC
                     KLEAWSEDGTALIGEVGELVITEPMPSMPVFFWGDDDGSRYRESYFEMFPGVWRHGDW
                     IEITDRGTAIIYGRSDSTINRGGVRMGTSEIYRAVLADDDVTDALVVDVPQENAENWM
                     PLFVVLREGVELGDDLAKRIKARVRTDCSPRHVPNTVHQIAEVPRTLSGKVLEVPVKR
                     ILMGTPPEEAASRDSLANPAALDPFTALADRRDPLT"
BASE COUNT         1066 a         3007 c         2560 g          970 t
ORIGIN      
        1 ctgggtgaag cgggtgtcgg cgagcagctc ggcctcctcc gagtcctcgg cggcgaacat
       61 cacctcgagc agcgggcgat cgaggtggcg gtcgagctcg gcgcagacct cgtcgagcgc
      121 ctgggcgaag accgggaact ccctgtgcag caccgagccc atcccggccc actgcgaccc
      181 ctggcccgag aacagaaacg cggtcgcgcc gccaccggcc gcgctccccc gcacgacgcc
      241 gttggccagc tcgccgcgct cgagcgcgcg caggctcgcc acgagctccg cgtggtcggc
      301 ggcgaccgcg acggcgcgct gcggcagctg ggcgcggcgg gtcgcgagcg ccgcggcgac
      361 ggcatacgca tcgagctccg ggcggcgctc caggaagccg gcgagccggc ccgcctgctc
      421 gccgagcgcc tgcggggtgg cggcggagac caggaagggc agggcccgcg ccgcggaagc
      481 ggcggcgtcg ccgtcggccg cggcggcgtc gccgtcggcc gcgccggcat cgccctcggc
      541 ggcatcgccc tcggcggcat cgccggcctc ggcgacctcg ccaccctccg catctcccac
      601 acccccatgc ggcgcatcgc cgttcagcgc cgacccgtcc ccgttcaccg cctgcgcctg
      661 aaggtcgggc gcgcccccgt tcgcgcccga cccctcaagc accggcccgt ctccgttcac
      721 gcgccgaggc gtgaggaccg gcggctcctc caagatcacg tgcgcgttcg tcccggacac
      781 gccgaacgac gacacgcccg cgcgccgcac ccgctccccc gccggccact cgaccggctc
      841 ctggagcagc tcgaccgcgc ccgccgacca gtccacgtgc ggcgtcggct cgcccgcgtg
      901 cagcgtcttc ggcagcagcc cgtggcgcag cgcctcgacc atcttgatca cgccgccgac
      961 gccggccgcc gcctgggtgt gacccgtgtt cgacttcagc gaccccaggt agagcggacc
     1021 gttcgagcga tcagcgccgt aggtggccag cagcgcctgc gcctcgatcg gatcgcccag
     1081 cgtcgtgccg gtgccgtggc cctcgaccgc gtctacgtcc gacggcgaga gcccggcatt
     1141 ggccagcgcc tggcggatga cccgctcctg cgacggcccg ttcggcgccg tcaacccgtt
     1201 cgacgcgccg tcctgattca cggcgctgcc gcgcacgagc gccagcacct cgtgcccgtt
     1261 cgcctgcgcc cgcgacagcg gctccagcac cagaaggccc gcgccctcgg accagcccgt
     1321 cccgtccgca tccgccccgt acgccttgca gcgcccgtcg accgcgagcc cgcgctggcg
     1381 cgagaactcc acgaagatcc ccgggttcgc catcaccgtc gcgccgcccg ccacggccag
     1441 ctcgcactcc ccctggcgca gcgcctggca cgcgaggtgc aaagcgacga gcgaggcgga
     1501 gcacgccgtg tccaccgaca ccgccggccc ctccagcccg aacatgtagg ccagccgccc
     1561 cgacgcgacg ctggtcaggc tgccggtgag cgcgtagccc tccgtgctcc ccgacgcctc
     1621 gtgcaggcgc ggcccgtagt ccgacgccat cacgccgacg aagacgcccg tctgcgtccc
     1681 gcgcaggctc gacggcgcga tcccggcgtc ctcgaacgcc tcccaggcac cctccagaag
     1741 caaccgctgc tggggatcca tcgccagcgc ctcgcgcggg gagatcgaga agacgtcggg
     1801 gtcgaactcg cccgcgtcgt agaggaagcc gccgtgccgc gtatagctcg tgccggagct
     1861 gtcgggatcc gggtcgtaga gcgcgtcgag cgcccagccg cgatcgtcgg ggaactcgcc
     1921 gatcgcgtcg cgccccgacg ccacgagctc ccacagcgcc tccgccgagg ccacgccgcc
     1981 gggatagcga cagctcatcc ccacgatcgc gatcggctcc tcggcgttcg acgcccgcgc
     2041 gaccgccgac gccttgcgac cggtgccagt cgcctcggcg agcaggaagt cggccaccgc
     2101 gaccggagtc gggtggtcga agagcaggct cgacggcagg cgcagcccgg tggccgccgt
     2161 cagcccgttg cgcagctcca ccgctcccgc cgagtcgaag ccgaggtcct tgaaggcccg
     2221 cttcgagtcc accgccgccg ccgagtcgtg cccgagcacg accgccgcct gggcgagaac
     2281 gacgtccagc acggtccgga cctggtcgtg cttgggcaga ccgtcgagcc gctgcgcgag
     2341 cgctccggcc ggccgcgccg catccgagcc gccgtcgccg tccacctcag cacccggagc
     2401 ccccgccaga gcggagacgg cgccggcgga ggacgaaccc agcccctcga gccagtggcg
     2461 ccggcgctgg aacgcgtagg tggggagatc gacccgccgc gcgcccgagc cggcgaacac
     2521 ggtccgccag tcgacggcgc cgccgcgcac gtagagcgcg gcgagcgacg tgaagaagcg
     2581 gtcgaggtcg ccgtggtcgc ggcgcaacgt gccgagcacc gccacgtcgg cggcgtcgtc
     2641 gcccagcatc tcctcgacgg tctcctgcgc gcccgccgtc atcaccggat gcgggctgat
     2701 ctcgaggaac gcaccgacgc cctcctcgat cagcgccctg acgacgtcgt agaagcgcac
     2761 ccgctggcgc aggttgcggt accagtagtc ggcgtcgagc cccgcggtgt cgatcgcctc
     2821 ggcctcgacc gtcgagaaga acggcaccgt gcccgagcgc ggctcgatcg gcgccaggtc
     2881 gcgcgccatc cgctcgcgga tctgctccac gcgcaccgag tgcgacgggt agtccaccgg
     2941 gatcttgcgc gcccaggtgc cgtcggcctc gcacgcggcc agcagctcgt cgagcgcctc
     3001 catctcgccc gagacgacca ccgaggcggg cccgttgacc gccgcgatcg acacccgctc
     3061 gccccagcgc ccgatcagct cctcggcgcc gcccggcgcc agcgccaccg acaccatgcc
     3121 gccgtggccc gcgaggtcgt cggcgaccgc ctggctgcgc agcgccacga cccgcgcggc
     3181 gtcctccagc gacagcgcgc cggccacgtg cgccgccgcg atctcgccct gcgagtggcc
     3241 gaccacggcc gcgggctcga cgccgtacga gcgccacagc gccgccagcg acaccatcac
     3301 cgcgaacagc gcgggctgca cgacgtccac gcgctcgaac gaagcagcgc cgtccacccc
     3361 gcgcagcacg tcctcgagcg accagtcgac gtgcggcgcg agcgcctccg cgcacgcgcg
     3421 catcgactcc gcgaataccg gcgccgagtc gagcagcccg agcgccatcc ccctccactg
     3481 cgatccctgc cccgggaaga cgaacacggt ccccccgccg cggctgctcg cctcgccggc
     3541 cacgacggcg gccgccggct cgccctgcga caggctgccg aggccctcca gcaggcgctc
     3601 gcggtcgccc ccgagcacca cggcgcggtg accgaaggcc gagcgcgtcg tcaccagtga
     3661 gtaccccacg tcgcccgcgc cgagatccgg ccggtccccc acgtgcgcca gcaggttgcg
     3721 cgcctggtcg cgcaccgcgc cctcgctcgt cccggacagc acccacggga ccacttcggg
     3781 gccgcccgcc gccggctcct ccgcggccgc gcccgcgtcg ccgcgctccg gcggctcggc
     3841 cagcaccacg tggcagttcg tcccgcccat gccgaacgag ctgacgcccg ccagcagcgg
     3901 acggtccggc gccggccagg agtcgagcga ccgctggacc cgcagatgca gctcctccag
     3961 cgggatcccg ggcgccggct cgctgaagtt gaggctgccg ggcagctccc tgtgctcgat
     4021 gcagagaatc gccttgatca gcccgacgat cccggccgcg ccctcgaggt ggccgacgtt
     4081 cgtcttggcc gaccccacca ccagcggccg ctcgccgtcg cggcccgcgc ccagcaccgc
     4141 gccgagcgcc gccgcctcca cctgatcgcc cagcggagta cccgtgccgt gcagctcgac
     4201 gtactggaca tccccgctct ccacgcccgc gcgctcgcag gcgagtcgca ggacctcctc
     4261 ctgggcgtcg cggtccggcg ccgtcaggcc gtcgccgccg ccgtcgttgt tcacggcggc
     4321 gccgcggatc acgcagtgga tcgggtcacc ctccgcgagc gcgctctcga gcggcttcag
     4381 cgccaccacg ccaccgccct cgccgcggac gtagccgttc gcgcgcgagt cgaaggtgaa
     4441 gcagcgcccg tccggggaca gcgcgccgag ccggtgggcg ctgatcgcgc tgtcgatgct
     4501 gatgttcagg ttgaccccgc ccgcgagcgc gagcgtggac tcgcccctga gcaggctctc
     4561 gcacgcgagc tggaccgcca cgagcgcgga cgactgcgcc gcgtccacgg tcaggctcgg
     4621 cccccgtagc cccaggatgt aggagacccg gttggcgatg atgccgcggt tcgtgccggt
     4681 gagcgcgtgc cgcgtgatgg cctcgccccc gtgccggcgc agcagctcgc catagtcgcc
     4741 ggagatcgcg cccacgaacg tgccggtccg gctgccgtcg atgtcgtcgg ggacgatccc
     4801 cgcgtcctcc agcgcctccc agctcagctc gaggatcagg cgctgctgcg ggtccatcac
     4861 cgcggcctcg cgcggcgaga tgccgaagaa ctcggcgtcg aactcgtcga cccggtcgag
     4921 gaagccgccg cgcagcaggc cgggcatcga cttctccgca ccggccaggg catccgtgtc
     4981 ccagcggtcg gccggagcgt ccccgatcgc gtcgcggccc tcgcggagca gctgccagaa
     5041 cgcgtccgga tccggggccg ccggcaggcg acaggccagg ccgaccaccg cgatcgcaga
     5101 gctctcagtc accgacgacc acccgccgca acggcggcga ggctcgcagc cgcgtacctg
     5161 ccgtcgctat agagcaatcg accgccaaag agcagcacta tccccccaac aacctggatt
     5221 ttcgagcgcc gttgggtgac ggcgacgcct atgaccgcgc tggcttcacg ctaaaggaat
     5281 gtcgccgtaa gcgggacgtt tccagtcaca cgtgaagcgc gccgttcaca cgagatgcgc
     5341 ggcgggcgga gcgacccctc accgggccgc ccggctacac ccggttgtcc tcgggcacca
     5401 cgtagcggtt gtagacgagg tcgcggatca cgtcgtcccc gccctcgatg atcgacacgt
     5461 gcctgatgtc gcggaccagc ttgtcgatga ccatctcgcg cgtgtaaccg aggccgccga
     5521 acatctcgga cccgacgccg gcgatcttcc agccggtctg cccacagaat gccttcgcgg
     5581 tgagcgccga cttgagcgtg cccttgcgga ggaagtgctc ggcggcgtcg tcacgttgca
     5641 tgatcgcgtc gaactcgcgc gcggcggcgc ggcacacatg ccgcatggcg tcgatctcca
     5701 tctccatctg accgagcttc gctgcgaaca ccgcgttgcc cgtgagcggc gagttgccga
     5761 gccgcttgcg ccgcgcgtac tccatgcaca ggtcgcgaac ccgccgggaa atcccgagcg
     5821 cggtgctcgc gatgaggatc cggctcgcgt tcaggccgat ctcgagaagc cgcaggcccg
     5881 ggccctggag cacgccctgc gacgggacgc ggcaacgctc gaacgacacc tggtaggtgc
     5941 ccgacgctcg caggccgatc atctcccagc gcttgaccat ccgcacgccc tggctgtcgc
     6001 cgggcacgac cacggccatg tgctcctcgg gattcgcggc cgacctcgcg atgacgatca
     6061 ggaagcccgc gaagcccgcg ttggtggaga agaacttctc gccgttgatg accacgtcct
     6121 cgccggcgcg cgcggcggtg gtcacgatct tggccagctc gctgccggcc gcgcgctcgc
     6181 tgccgagcgt cgcgcagaag ccgccatccc tcgggatcgt ggtgaggtag cgctccttga
     6241 cctcgtcgct cccgtagagc tgcaccgtgg tcgtgccgag gatcgagatg aactgcgtga
     6301 acgccacgcc cgcgtcgccg taggccagct ccgagacgat gtccacgctc tcctccagcc
     6361 cgagccccct gccgccgtac tcctcgggga gccaccagcc gctcaacccg agggagtgga
     6421 acgcctcgtt ggccggcacc gggacgtccg ggagcgtgtc gaagtgcgcc tgctggggca
     6481 gcagctcggt cttcgcgaac tcgcggacct gcggcaggat ctcctccaga accgcgctcc
     6541 cgccgccgct ttgaatgccc tcgtccagca agaagcagcc ccccttacgc ccgttgccca
     6601 ggttcgtcgc caggcagtgt gcccattcag aggcccggct gcatcacacg tgaattgcgc
     6661 gattcacgtg agagggtccc ggcggtccgc gagcgccgtg aacgggtcga gcgccgcggg
     6721 gttcgcgagg ctgtcgcggc tcgcggcctc ctcgggcggg gtgcccatca ggatgcgctt
     6781 cacgggcacc tcgagcacct tgcccgacag cgtgcgcggc acctcggcga tctggtgcac
     6841 cgtgttgggc acgtggcgcg gcgagcagtc cgtccgcacg cgggccttga tgcgcttggc
     6901 gaggtcgtcg ccgagctcga ccccctcgcg cagcaccacg aacagcggca tccagttctc
     6961 ggcgttctcc tgcgggacgt ccaccacgag ggcgtcggtc acgtcgtcgt cggcgagcac
     7021 ggcgcggtag atctcgctgg tgcccatgcg cacgccgccg cggttgatgg tcgagtcgct
     7081 gcggccgtag atgatcgcgg tgccgcggtc ggtgatctcg atccagtcgc cgtggcgcca
     7141 gacgcccgga aacatctcga agtagctctc gcggtagcgc gagccgtcgt cgtcgcccca
     7201 gaagaagacc ggcatgctcg gcatgggctc ggtgatgacc agctcgccga cctcgccgat
     7261 cagcgcagtg ccgtcttccg accacgcctc gagcttgcag ccgaggctcg cggcctggag
     7321 ctcgccccgg tggacgggca gcgtggggac gccgcccacg aacgcggtgc acacgtcggt
     7381 gccgccggag gtgctgaaca gccacgtgtc ggatccgagc ttgtcgtaga tccaggcgaa
     7441 cgcctcgggc gacagcgggg atcccgtgct gcccaccgcg tgcagggccg agaggtcgcg
     7501 gccggcggcc ggctcgacgc cctccttcat gccgccggcg aagaacgccg cgccgccccc
     7561 gaagatcgtc atgccggtct cggcggcgag gtcccacagc acg
//
LOCUS       DASVPL010000073         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_48397, whole genome shotgun sequence.
ACCESSION   DASVPL010000073 DASVPL010000000
VERSION     DASVPL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48397"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VF517_03445"
     CDS             complement(<1..586)
                     /locus_tag="VF517_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8052020.1"
                     /translation="MRFVSRAALAALELSPFPRRFSRLPREKRARHIERMESSRSLNR
                     RSIVLLTKTLTCVTYTRDPRVRAAVGWEPRCELAEGAAAEAAVPLRCAAPPPAGEALR
                     CDVVVVGSGAGGATAARVLAESGLDVVVLEEGEHHDARDFTTDPVHALRTLYRDGGLT
                     ACDGRPPIPLPVGRCVGGTTVINSGTAVRPPGDVL"
     gene            complement(773..1204)
                     /locus_tag="VF517_03450"
     CDS             complement(773..1204)
                     /locus_tag="VF517_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052021.1"
                     /translation="MATMRLLVASFAVVGILFIATPDGVVHRIEQFGELFGDFARGAE
                     TEQKMWLGLAFAYMTVITGLALVVSLDVVRFRPLLLVLAAGKLASSLTTGAFFAFDDD
                     VFIYLLNFLVDGSLVGTSLFCWSLAGRVETAREAGALAAPG"
     gene            1197..>1915
                     /locus_tag="VF517_03455"
     CDS             1197..>1915
                     /locus_tag="VF517_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052022.1"
                     /translation="MATTMRSAVAGLIAAGILTPAASADPLLDRHRPVLRYDSSERDR
                     STAVDAIVPRLDPDRERVDLEPEQPGREVVYGHRARGGDGRTWLQYWLFFLDNPQDRG
                     ILRGGRHEGDWELVQLRLDAAGRPDRVTFAQHSWAEGCEWSDTRREGGAPVAYVANAS
                     HALYSRPKTHDRQWPDPDDEADGRGRRVRPPVVPIADRDPAWTRWPGRWGDSEAGIVP
                     IEQSSPRGPAFQERRPWQDPSA"
BASE COUNT          289 a          738 c          674 g          214 t
ORIGIN      
        1 gcaggacgtc gccaggcggc cgcacggcgg tgccggagtt gatgacggtc gtgccgccca
       61 cgcagcgtcc gaccggcagc gggatcggcg ggcggccgtc gcacgcggtg aggccgccgt
      121 cgcggtagag cgtgcgcagc gcgtggaccg gatcggtcgt gaagtcgcgc gcgtcgtggt
      181 gctcgccctc ctcgagcacg accacgtcga ggccggattc ggcgagcacg cgcgctgcgg
      241 tcgcaccgcc cgcccccgag ccgaccacca ccacgtcgca acgcagcgcc tcgccagccg
      301 ggggcggcgc ggcacagcgc agcgggaccg ccgcctccgc ggccgctccc tccgccagct
      361 cgcagcgcgg ctcccatccg accgccgcgc gcacccgcgg gtcgcgcgtg tacgtgacgc
      421 acgtgagcgt cttggtgagc aggacgatcg agcggcggtt gagcgaccgg ctcgactcca
      481 tgcgctcgat gtggcgggcc cgcttctcgc gcgggaggcg cgaaaaccgg cgggggaagg
      541 gggagagctc gagcgcggcg agcgcggcgc gggagacgaa ccgcatccgc ggtggggctc
      601 ccgccagcag cgccatcgcg ggctcgacga catcgattgg agggttttgc cgcctatggc
      661 cggcaaaggc tccaatcggt gcggaatcag cggctcccac cgccggcggc accgcataac
      721 cacccggcag cagcgcctcc gcgaacgcgc cgagcgtgcg ccgcgcggct cttcagcccg
      781 gcgcggccag cgcgcccgct tcgcgcgccg tctcgacccg tcccgccagc gaccagcaga
      841 acagcgacgt gccgaccagg ctgccgtcca ccaaaaagtt caggaggtag atgaagacgt
      901 cgtcgtcgaa ggcgaagaac gcgcccgtcg tgagcgacga cgcgagcttg cccgccgcga
      961 gcaccagcag cagcggccgg aaccgcacca cgtcgagcga caccacgagc gccagcccgg
     1021 tgatcaccgt catgtaggcg aacgcgagcc cgagccacat cttctgctcg gtctccgcac
     1081 cccgtgcgaa gtcgccgaac agctcgccga actgctcgat ccgatgcact acgccgtccg
     1141 gcgtggcgat gaacaggatt ccgacaaccg cgaaggacgc gacgagtagg cgcatcgtgg
     1201 ccacgacgat gcgttcggcg gtcgcgggac tcatcgcggc cgggatccta acccccgccg
     1261 catccgcgga cccgctgctc gaccgccacc ggccggtgct ccgctacgac agctccgagc
     1321 gcgaccgcag caccgccgtc gacgcgatcg tgccgcggct cgacccggac cgcgagcggg
     1381 tggacctcga gcccgagcaa ccgggccgtg aggtcgtcta cggccaccgc gcccgcggcg
     1441 gcgacggacg cacctggctc cagtactggc tcttcttcct cgacaacccc caggatcgcg
     1501 ggatcctgcg cggcggcagg cacgagggcg actgggagct cgtccagctc cgcctcgatg
     1561 ctgccgggcg ccccgaccgc gtcaccttcg cgcagcactc ctgggccgag ggctgcgagt
     1621 ggagcgacac cagacgcgag ggcggcgcgc cggtggcgta cgttgccaac gcctcgcacg
     1681 cgctctactc gcgccccaag acccacgatc gccagtggcc cgatcccgac gacgaggccg
     1741 acggccgcgg ccgtcgcgtg cgccctccgg tcgtcccgat cgccgaccgc gaccccgcct
     1801 ggacccgctg gccaggccgg tggggcgaca gcgaggccgg catcgtcccg atcgagcagt
     1861 cgagcccgcg cgggcccgcc ttccaggagc gccgcccgtg gcaggatccg tccgc
//
LOCUS       DASVPL010000074        10212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_48566, whole genome shotgun sequence.
ACCESSION   DASVPL010000074 DASVPL010000000
VERSION     DASVPL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48566"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF517_03460"
     CDS             <1..543
                     /locus_tag="VF517_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8052023.1"
                     /translation="PTPAPDPRWAWKALPGPRWWAHLAHTLTHDDIGAADQQRREAAM
                     HGLEIRHPLRDPELIDLVLALPPEPGFDPHLDRPLIRAALAGELPEATLRDSHKPFFN
                     SLLDGALSGTDAPALRALLRDPHPELAALARTEAIQELLEPSRGGVRTPTWGVDLWRL
                     GTLELWLEHRYGKPPNDGSG"
     gene            609..743
                     /locus_tag="VF517_03465"
     CDS             609..743
                     /locus_tag="VF517_03465"
                     /inference="COORDINATES: protein motif:HMM:NF033521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso RiPP family leader peptide-containing
                     protein"
                     /protein_id="HEX8052024.1"
                     /translation="MQYEAPEITDYGTLTDLTAGTQDGNLTDRDFPVNTPRDEITFSD
                     "
     gene            694..1854
                     /locus_tag="VF517_03470"
     CDS             694..1854
                     /locus_tag="VF517_03470"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8052025.1"
                     /translation="MTSRSTRPATRSPSPTSPSGPVFLLAGCTARRAALSERARRALA
                     ETDHEALAYELARRRLLPLVGTRALEAAGDVCPPSFHAHVRAAVTAARARGIAVEAEL
                     RRTVALLADHGIRALPLKGPLLAAEAHGDYGLRETDDVDLLVPPARLHEAADLLAAAS
                     YARMDDPIRPNGLPDLHLALQHPQGPRIELHWRVHWYEEAFSEDMLARAEPGPDGLLR
                     PRPDDLAAALLLFYARDGLYGVRLAADIAGWWDRHGDALPPGFLEGHARCYPALAPAL
                     TAAAEAAERLTGVPATGWLGSAAVRGRRVEIATRLSNWTQSGDRDQLAANISLADGLL
                     GPRGSVGAFARRQLQPRAGALVPHATKTVARYAIALWRVRGGRWWSPLPDAR"
     gene            complement(1866..3260)
                     /locus_tag="VF517_03475"
     CDS             complement(1866..3260)
                     /locus_tag="VF517_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052026.1"
                     /translation="MDRVPRSALLAAVCVLLGVATLLIPASPSYDPWSWIIWGREVLD
                     GDLRTATGPSWKPLPVLFTAPLSLFGDAAPALWLVVARAAQFAAAVPAALIGFRLAGR
                     LGAILSGGLLLSFTALWESTLQGHAEGALVLCVLAAVERHLAARHGPAFAWALGAALL
                     RPEAWAFLGLYAAWLALRQRGRLAWIVPALAFVPLLWFLPEQWGSGSLSRGAERAQLA
                     GADSPGAAEHPAVEVVRNAFDLSPGIVFLGLAAGLAACWVRRPSRRALLLAIALAALG
                     VGWVAQVAVMSELGYSGIDRYLLPAVAIAHVLAGVGLAALLAGLVGARRSAATGAAVL
                     LVLLGAGLMVRAGSADWPRSMDQIERIDAITDEFDTAVARAGGRPRLERCGAISASQL
                     ATPVVAWDFDRHLEQVTNHPRVPGSILRARLRGDLPVDPPLNALARTPGRTVLARTRW
                     WTVEAACSPAAAGP"
     gene            complement(3341..4234)
                     /locus_tag="VF517_03480"
     CDS             complement(3341..4234)
                     /locus_tag="VF517_03480"
                     /inference="COORDINATES: protein motif:HMM:NF009125.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8052027.1"
                     /translation="MSYETILYAVEDAVATITLNRPEQLNTIVPPMPDELEAAVHRAV
                     RDEAVKVIVLRGAGRSFCAGFDFGDGFHHWDEGLTTDGEWDPGKDFVANTSPATAPVP
                     KIMSIWRSPKPVIAQVHGWCVGGGSDMALCADLVVASDDARIGTPYARMWGCYLTGMW
                     IYRLGMARVKWHALTGHPLSGAEAAEAGLINVSVPFERLEGEVAGLAARLASVPSSQL
                     AAMKLVVNQAYEGMGIAQTQVLGPILDGLMRNTPDARRWIEHAQEHGVGAAIADRDGP
                     FGDYSQAPADQKPDPSHVIHP"
     gene            4311..5231
                     /locus_tag="VF517_03485"
     CDS             4311..5231
                     /locus_tag="VF517_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052028.1"
                     /translation="MRGPIAAVLVLGALVLAAPAAAVTTTLGRLDPSSDPNSGSCAYV
                     RALYIADDTPPNRDEDGYPGGWIPAGGGVITSWTTSKHPPGSRFRLAIVKRNNQLPET
                     SHGVVAVAWSAIETVTVPDGRPQTFRTNLRVTGEERQYIALDAVEDDYIGDSCHYGTP
                     FANFVYVAGEQTDSKGYTPFVTGDGYDHNRVNLEVTLDRGDNPPPQPPAGEASQPSGD
                     PMPPAPTPSGSEASQPTPSDPGPSQPGPSGPATSQPAPGSPSADAAHERRIALKRHCR
                     RLKSRLGARHPKARRACRRWHQARDAAARG"
     gene            complement(5235..6116)
                     /gene="era"
                     /locus_tag="VF517_03490"
     CDS             complement(5235..6116)
                     /gene="era"
                     /locus_tag="VF517_03490"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX8052029.1"
                     /translation="MTRSGFVALAGRPNVGKSTLLNALVGEKVAIVSNRPQTTRRAIR
                     GIATLDEAQLVLVDLPGVQRPRDPLTERMQARMEQELRDSDAALMLLNGQQGVGPGDR
                     FIARAIASAGVPHVIAVNKVDVLDRAKTLDALEQAATLELPGDVFPVSALRGSGVPEL
                     VEALIAFLPEGPFLYPPEDRTDLSLTVRLAEIVREQVLARTRDELPHAVEVEVDDPEE
                     RDDGLVVVVARAWAETESQKGILIGSGGKMVRAIGSAARKEMEALLGSRVHLDLSVRV
                     RKGWRRDDALLDRLGID"
     gene            complement(6113..6475)
                     /gene="ybeY"
                     /locus_tag="VF517_03495"
     CDS             complement(6113..6475)
                     /gene="ybeY"
                     /locus_tag="VF517_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX8052030.1"
                     /translation="MTLDIESPPELRGAVEAALAAAGLAEGHVAVGLVGEDEIRRLNR
                     EHRGRDEPTDVLSFPVDEAGPAAGPRELGDVVICPEHTADLTEATVHGVLHLCGYDHE
                     TDAGEMFALQDRVMEGLR"
     gene            complement(6472..7416)
                     /locus_tag="VF517_03500"
     CDS             complement(6472..7416)
                     /locus_tag="VF517_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX8052031.1"
                     /translation="MRTQVELSNDVAKELAGPGDAIMKTLEGHLGADIFLRGNILTLD
                     GEESDVAAAEEVVREVSDLVRAGHEIAPGTIAAITGALDQHASPSAILDDVIWRHRGL
                     KVAPKTVNQKRYVDAIREQTIAVGIGPAGTGKSFLAVAMAVAALSRREVNRIVLTRPA
                     VEAGERLGFLPGDLMAKVDPYLRPLFDALYSMLEPEKVNQYLDRGTIEVAPLAFMRGR
                     TLNDSFIILDEAQNTSPEQMKMFLTRLGFNSKMVVTGDITQIDLEGDRRSGLVVIGDI
                     LGQVEGIEFVRFGGEDVVRHKLVQRIVAAYDQYGQRTS"
     gene            complement(7416..7856)
                     /locus_tag="VF517_03505"
     CDS             complement(7416..7856)
                     /locus_tag="VF517_03505"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX8052032.1"
                     /translation="MSLLEQVQNDATTALKAGERERCNALRLVVSALQTAHKEGSPSE
                     ADEVAVLQRERKRRLESADAYREAGRDDLAGTEQAEAELISSYLPEQMSDEELGAIVG
                     DVVAETGAASPKEMGRVMSMVMPRVQGRADGKRVSALVKEKLTA"
     gene            7884..8561
                     /locus_tag="VF517_03510"
     CDS             7884..8561
                     /locus_tag="VF517_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX8052033.1"
                     /translation="MRPSIATFDCYGTLIDWEGGFGAFLYDKALRHGDTELVPGRELR
                     ERFETIQFELIQGDYRPYKEVLAETLKRLCAERGWPYEDGDAAAVLRSMRSWQPFPDT
                     RPALRRVRAAGIRLMIVSNTDRDIIEHSLRHLEVPFDEVQVAEDVRAYKPADAVFTAA
                     LERIGEPPERVLHVAFGFKYDIGPATRAGMQTAWVNRHQEPPPGSTPYDHEWRDLWGL
                     ASLAEAD"
     gene            8568..9836
                     /locus_tag="VF517_03515"
     CDS             8568..9836
                     /locus_tag="VF517_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8052034.1"
                     /translation="MSLSPPAISPGWELGDLHTAALLAIGLAIILAAVAISREHERTF
                     SASVLYVAIGAAGALALSLLDVEGLDPEGDHLVMERLSELALIAAVFSAGLTIERHVQ
                     RRSLVSLATLLVVVMPLTIAAIALFGMWAMGLSFGAALLLGAVLAPTDPVLAGDVGLS
                     APGGEVYGEPRLSLHTEAGFNDGLASPFVVLGLFVAELGGSDWVGEWIGADLLYGAGV
                     GLALGAAAGFAAAAGLTAARGAGVITGRLEGFVTIGLMLAIYGASEAVGTYGLLAVFA
                     AGFTFRRYEFDHEMHHPVHQGAHAAGKALELIVLLMLGTMLTTAGLGAPGVEGWLLVP
                     VLLLLIRPALVFATAGRRFMDVRGRLFLAFFGVRGVAALFYAAVVVEAGVLEPGEQHV
                     VVWTTIVCVVCSIVVHGVSAAPLTRRWLGG"
     gene            9898..>10212
                     /locus_tag="VF517_03520"
     CDS             9898..>10212
                     /locus_tag="VF517_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8052035.1"
                     /translation="MHVDEYILIASALLAGGLGASLMARRLRLPSLLLFLGLGMAVGS
                     DGAGWIEFNDYELARGIGVIALSLILFEGGLSAGFAEIRPVLRPAIGLALLGTLATAV
                     ITG"
BASE COUNT         1364 a         3911 c         3539 g         1398 t
ORIGIN      
        1 ccaacccccg cccccgaccc gcgctgggcc tggaaggccc tccccggccc gcgctggtgg
       61 gcgcacctcg cccacacgtt gacccacgac gacatcgggg ccgcggacca gcagcgccgc
      121 gaggccgcca tgcacgggct cgagatccgc cacccgctgc gcgaccccga gctgatcgac
      181 ctcgtgctgg cgctgccccc ggagcccggc ttcgaccccc acctcgaccg tccgctcatc
      241 cgcgccgcgc tcgccggcga gctgcccgag gccacgctcc gcgactcgca caagccgttc
      301 ttcaactcgc tgctcgacgg cgcgctgtcc ggaaccgacg ctcccgccct gcgcgcgctg
      361 cttcgcgacc cccacccgga gctggccgca ctggcgcgaa ccgaggcgat ccaggagctg
      421 cttgaaccgt cccgaggagg ggtgcgtaca cccacctggg gcgtcgacct ctggcggctt
      481 ggcacgttgg agctctggct ggagcaccgg tacggcaagc ctccgaacga cggttccggt
      541 tgagaaaggc gttttggatc cctatattgc ggcgatccag gacggggaca cctatgcagg
      601 aggggcaaat gcagtacgaa gcgccggaga tcactgacta cggcacgctg acggatctga
      661 ccgcgggcac gcaggacggc aacctcaccg accgtgactt cccggtcaac acgccccgcg
      721 acgagatcac cttctccgac tagcccctcc ggcccagtct tcctgctcgc gggctgcacg
      781 gcccgccgag ccgccctgag cgaacgcgcc cgccgcgcgc tcgccgagac cgatcacgag
      841 gcgctcgcgt acgagctcgc ccggcgccgg ctgttgcccc tcgtcggcac acgcgcgctc
      901 gaggcggccg gtgacgtctg cccgccgagc ttccacgcgc acgtgcgcgc cgcggtcaca
      961 gccgcgcgcg cccgcggcat cgcggtcgag gccgagctgc gccgcaccgt ggccctgctc
     1021 gccgaccacg gcatccgcgc gctcccgctg aagggaccgc tgctcgccgc cgaggcgcac
     1081 ggcgactacg gcctgcgcga gaccgacgac gtggacctgc tcgtcccccc cgcccgcctg
     1141 cacgaggccg cggacctcct ggccgccgcg agctacgcgc gcatggacga cccgatccgc
     1201 ccgaacgggc ttccggacct gcacctcgcg ctgcagcacc cgcaggggcc gcggatcgag
     1261 ctgcactggc gcgtccactg gtacgaggag gccttctcgg aggacatgct cgcgcgcgcc
     1321 gagcccggac cggacggcct cctacgcccc cgccccgatg acctcgccgc ggcgctgctc
     1381 ctcttctacg cccgcgacgg gctctatggg gtccgcctcg ccgccgacat cgcaggctgg
     1441 tgggaccgcc acggcgatgc gctgccaccc ggattcctcg agggccacgc ccgctgctac
     1501 cccgcgctgg cgcccgcgct gaccgccgcc gccgaggcgg cagagcggct caccggcgtc
     1561 ccggcgacgg gctggctcgg ctcggcggcc gtgcgcggcc gccgcgtcga gatcgccacc
     1621 cgcctgtcga actggaccca gagcggcgac cgcgaccagc tggccgccaa catctcgctc
     1681 gccgacgggc tgctcggacc gcgcgggtcg gtcggggcgt tcgcccgccg gcagctccag
     1741 ccgcgcgcgg gcgcgctggt gccgcacgcc accaagaccg tcgcccgcta cgcgatcgct
     1801 ctctggcgcg tgcgcggcgg ccgctggtgg tcgccgctgc ccgacgcgcg ctagaccgcc
     1861 gagcgtcagg gcccggctgc ggccggcgag cacgccgcct cgaccgtcca ccacctggtg
     1921 cgagccagga cggtccggcc gggcgtccgg gcgagagcgt tcagaggcgg gtcgaccggt
     1981 aggtcaccac gcagccgcgc acggaggatc gagcccggca cccgcggatg gttcgtgacc
     2041 tgctccaggt ggcggtcgaa gtcccacgcg accaccggcg tggcgagctg gctggcgctg
     2101 atcgccccgc accgctcgag gcggggcctg ccgcccgcgc gcgcgaccgc ggtgtcgaac
     2161 tcgtcggtga tcgcgtcgat ccgctcgatc tggtccatgc tgcgcggcca gtcggcgctc
     2221 cccgcccgga ccatcagccc cgcgccgagt agcaccaaca gcacggcggc gcccgtggcg
     2281 gccgagcgcc gggcgccgac cagccccgcc agcaacgcgg cgagcccaac acccgcgagc
     2341 acgtgcgcga tcgccaccgc gggcagcagg tagcggtcga tccccgaata cccgagctcg
     2401 ctcatgaccg ccacctgcgc gacccagccc actccgagcg cggccagcgc gatcgccagc
     2461 agcagcgcgc gccgtgacgg ccggcgcacc cagcaggccg ccagtccggc ggcgagcccg
     2521 aggaacacga tccccggtga caggtcgaac gcgttgcgca ccacctccac ggccgggtgc
     2581 tcggccgcgc ccggcgagtc cgccccggcg agctgcgcac gctctgctcc gcgcgagagg
     2641 cttcccgacc cccactgctc cgggaggaac cacagcagcg gaacgaacgc gagcgccggc
     2701 acgatccacg cgagccggcc tcgctgccgc agcgcgagcc acgccgcgta gaggccgagg
     2761 aacgcccagg cctcgggtcg aagcaacgcg gctccgaggg cccaggcgaa cgcggggccg
     2821 tggcgcgcgg cgaggtggcg ctcgaccgcg gcgagcacgc acaggacaag cgcgccctcg
     2881 gcatggccct ggagcgtcga ctcccacagc gccgtgaacg agagcaggag cccgccgctg
     2941 aggattgcgc cgagccggcc ggcgaggcga aacccgatca gtgccgcggg gacggcggct
     3001 gcgaactgcg ccgcgcgcgc gaccacgagc cacagcgcgg gcgccgcgtc accgaacagc
     3061 gacagcggcg cggtgaacag gaccgggagc ggcttccacg acgggccggt cgcggtccgc
     3121 aggtcgccgt cgaggacctc gcggccccag atgatccagg accaggggtc gtaggacggc
     3181 gatgccggga tcaagagcgt cgcgacgccg aggagcacgc agaccgccgc gaggagggcc
     3241 gatctcggaa cgcggtccaa gaggctgacc gtacccgcac cgccgccggc ccggtgccaa
     3301 agggtcgccg tacgaacgta ccttggcgcc cggcccgtgg tcaggggtgg atcacgtggg
     3361 aaggatcggg cttctggtcg gccggcgcct ggctgtagtc gccgaacggt ccgtcgcggt
     3421 ctgcgatggc cgcccccacg ccgtgctcct gcgcgtgctc gatccagcgc ctggcgtcgg
     3481 gcgtgttgcg catcagcccg tcgaggatcg ggccgagcac ctgcgtctgc gcgatcccca
     3541 tgccctcata cgcctggttg acgaccagct tcatcgcggc gagctgcgag gacggcaccg
     3601 acgcgagtcg cgcggccagt cccgcaacct ccccctcgag ccgctcgaac ggcaccgaga
     3661 cgttgatcag gcccgcctcc gccgcctccg cgcccgacag cggatggccg gtgagcgcgt
     3721 gccacttgac ccgcgccatg ccgagccggt agatccacat gccggtcagg tagcagcccc
     3781 acatgcgcgc gtagggggtc ccgatgcggg cgtcgtcgga ggcgacgacg aggtccgcgc
     3841 agagcgccat gtcgctgccg ccgcccacgc accacccgtg cacctgcgcg atcaccggct
     3901 tgggcgagcg ccagatcgac atgatcttcg gcaccggggc cgtcgccggc gaggtgttgg
     3961 cgacgaagtc cttgccgggg tcccactcgc cgtcggtggt gagtccctcg tcccagtggt
     4021 ggaagccgtc gccgaagtcg aacccggcgc agaacgagcg cccggcgccg cgcagcacga
     4081 tcaccttcac ggcctcgtcg cgcaccgccc ggtgcacggc cgcctcgagc tcgtcgggca
     4141 tcggcggcac gatggtgttc agctgctcgg gccggttcag cgtgatcgtg gccaccgcgt
     4201 cctcgaccgc gtacaggatc gtctcgtagc tcatcgggcg gagttcgccg ccggggcgcc
     4261 gattcccgcg ggttcatgct cgggcggttc gtgggtatca cccacccacg atgaggggcc
     4321 cgatcgccgc agtcctcgtg ctgggcgcgc tcgtgctggc cgcgcccgcc gcggcggtga
     4381 ccaccacgct cggccgcctc gatcccagca gcgacccgaa cagcggctcg tgcgcgtacg
     4441 tgcgcgcgct ctacatcgcc gacgacacgc cgccgaaccg cgacgaggac ggctaccccg
     4501 gcggctggat accggcgggc ggcggcgtga tcacgtcgtg gaccacgagc aagcacccgc
     4561 ccgggtcgcg cttccgcctc gcgatcgtga agcgcaacaa ccagctcccc gagacgagcc
     4621 acggggtggt cgcggtcgcg tggagcgcaa ttgagacggt caccgtgccc gacgggcgcc
     4681 cgcagacctt caggaccaat ctccgagtga ccggcgagga gcgccagtac atcgcgctcg
     4741 acgccgttga ggacgactac atcggcgata gctgccacta cggcacgccg ttcgcgaact
     4801 tcgtctacgt cgccggcgag cagaccgact ccaagggcta cacgccgttc gtcaccggtg
     4861 acggctacga ccacaaccgc gtgaacctcg aggtgacgct cgaccggggc gacaacccgc
     4921 cgccgcagcc gcccgccggg gaagcgagtc agccgagcgg cgacccgatg cccccggccc
     4981 ccacgcccag cggctcggag gccagccagc ccactcccag cgaccccggc ccgagccagc
     5041 ccggcccgag cgggcccgcc acgagccagc ccgccccggg cagtccctcc gccgacgccg
     5101 cgcacgagcg ccggatcgcg ctgaagcggc actgccgccg gctgaagagc cggctcggcg
     5161 cgcgccaccc gaaggcgcgg cgcgcgtgcc gccgctggca ccaggcgcgc gacgccgcgg
     5221 cgcgcggcta ggcgctagtc gatcccgagc cggtcgagca gcgcgtcgtc gcggcgccag
     5281 cccttgcgca cgcgcaccga caggtcgagg tgcacgcggc tgccgagcag cgcctccatc
     5341 tccttgcgcg cggcgctccc gatcgcccgc accatcttgc ccccggagcc gatcaggatg
     5401 cccttctgcg actcggtctc ggcccaggcc ctggcgacca ccaccacgag gccgtcgtcg
     5461 cgctcctccg ggtcgtcgac ctcgacctcg accgcgtgcg gcagctcgtc gcgcgtgcgg
     5521 gccagcacct gctcgcgcac gatctcggcg aggcggaccg tcagcgacag gtcggtgcgg
     5581 tcctcgggcg ggtagaggaa cggtccctcc ggcaggaagg cgatcagcgc ctcgaccagc
     5641 tccggcaccc ccgacccgcg cagcgcgctc acggggaaga cgtcgccggg cagctcgagc
     5701 gtcgccgcct gctcgagcgc gtccagcgtc ttcgcgcggt cgagcacgtc gaccttgttc
     5761 accgcgatca cgtggggcac ccccgcactc gcgatcgcgc gcgcgatgaa cctgtccccc
     5821 ggcccgacgc cctgctggcc gttcagcagc atcagagcgg cgtccgagtc gcgcagctcc
     5881 tgctccatcc gggcctgcat ccgctcggtg agcgggtcgc gcgggcgctg cacccccggg
     5941 aggtcgacca gcacgagctg cgcctcgtcg agcgtcgcga tcccgcggat cgcgcggcgc
     6001 gtggtctgcg gccggttcga gacgatcgcg accttctccc ctacgagcgc gttcaagagc
     6061 gtcgacttgc ccacgttggg gcggccggcg agcgcgacga agccgctgcg cgtcaccgca
     6121 ggccctccat cacgcggtcc tgtagggcga acatctcgcc ggcgtcggtc tcgtggtcgt
     6181 agccgcagag gtgcagcacg ccgtgcacgg tggcctcggt caggtcggcg gtgtgctcgg
     6241 ggcagatcac cacgtcgccg agctcgcgcg ggccggccgc cggccccgcc tcgtcaaccg
     6301 ggaacgagag cacgtcggtg ggctcgtcgc ggccgcggtg ctcgcggttg agccgccgga
     6361 tctcgtcctc ccctaccagc cctaccgcca cgtggccctc ggcgaggccc gccgccgcga
     6421 gcgcggcctc gaccgcgccg cgcagctcgg gcggcgactc gatgtcgagc gtcagctcgt
     6481 cctctgcccg tactggtcgt aggccgccac gatccgctgc acgagcttgt ggcgcaccac
     6541 gtcctcgccg ccgaagcgca cgaactcgat tccctcgacc tggcccagga tgtcgccgat
     6601 cacgacgagt ccgctgcgcc gatcgccctc gaggtcgatc tgagtgatgt cgccggtcac
     6661 gaccatcttc gagttgaacc cgagccgcgt gaggaacatc ttcatctgct cggggctcgt
     6721 gttctgcgcc tcgtcgagga tgatgaaaga gtcattaagc gtgcggccac gcataaacgc
     6781 taaaggcgcc acctcgatcg tcccgcggtc gaggtactgg ttgaccttct ccggctcgag
     6841 catgctgtag agcgcgtcga agagcgggcg caggtatgga tcgaccttcg ccatcaggtc
     6901 gccggggagg aatcccagcc gctcgccggc ctcgaccgcc ggccgcgtca gcactatgcg
     6961 attcacctcg cgccgcgaga gcgccgccac cgccatcgcc accgccagga acgacttgcc
     7021 cgtgcccgcc gggccgatgc cgaccgcgat cgtctgctca cggatcgcgt ccacgtagcg
     7081 cttctggttc acggtcttcg gcgcgacctt cagcccgcgg tggcgccaga tcacgtcgtc
     7141 gaggatcgcg ctcgggctgg cgtgctgatc gagcgcgccc gtgatcgcgg cgatcgtgcc
     7201 gggggcgatc tcgtgcccgg cgcgcaccag gtcggacacc tcgcgcacca cctcctcggc
     7261 ggccgcgacg tcgctctcct cgccgtcgag ggtgaggatg ttcccgcgca ggaagatgtc
     7321 ggcgccgagg tgcccctcga gcgtcttcat gatcgcatcg ccggggccgg ccagctcctt
     7381 ggcgacgtcg ttggacagct cgacctgcgt acgcactagg cggtgagctt ctccttgacc
     7441 agcgcgctca cgcgcttgcc gtcggcgcgc ccctgcacgc gcggcatgac catcgacatc
     7501 acgcggccca tctccttcgg cgaggcggcg ccggtctcgg ccaccacgtc gccgacgatc
     7561 gcgccaagct cctcgtcgga catctgctcg ggcaggtagc tcgagatcag ctcggcctct
     7621 gcctgctcgg tgccggcgag gtcgtcgcgg ccggcctcgc ggtaggcatc ggcggactcg
     7681 agccggcgct tgcgctcgcg ctggagcacc gccacctcgt ccgcctcgct cggcgagccc
     7741 tccttgtggg ccgtctgcag cgcggagacg accagccgca gcgcgttgca gcgctcgcgc
     7801 tcgcccgcct tcagcgcggt ggtggcgtcg ttctgcacct gttcgaggag ggacatcgcc
     7861 tgtcaagtct aggctggacg gccgtgcggc ccagcatcgc cacattcgac tgctacggca
     7921 ccctcatcga ctgggagggc ggcttcggcg cgttcctcta cgacaaggcg ctccgccacg
     7981 gcgacaccga gctcgtgccc ggccgcgagc tgcgcgagcg cttcgagacg atccagttcg
     8041 agttgatcca gggcgactac cgcccgtaca aggaggtcct cgcggagacc ctgaagcgcc
     8101 tctgcgccga gcgcgggtgg ccctacgagg acggcgacgc cgccgcagtg ctgcgctcga
     8161 tgcgcagctg gcagccgttc cccgacaccc gccccgccct gcggcgcgtg cgcgcggcgg
     8221 gcatccgcct gatgatcgtc tccaacaccg accgcgacat catcgagcac agcctgcgtc
     8281 acctggaggt gccgttcgac gaggtccagg tggccgagga cgtgcgcgcc tacaagccgg
     8341 ccgacgccgt cttcacggcc gccctcgagc gcatcggcga gccgcccgag cgcgtgctgc
     8401 acgtcgcgtt cgggttcaag tacgacatcg gccccgctac ccgcgccggc atgcagaccg
     8461 cctgggtcaa ccgccatcag gagcccccgc ccggatcgac gccctacgac cacgagtggc
     8521 gcgatctctg gggactggcg agcctggccg aggccgatta ggcgggcatg agcctcagcc
     8581 ctcccgccat ctcgcccgga tgggagctcg gcgaccttca caccgcggcc ctgctggcca
     8641 tcgggctcgc gatcatcctc gcagcggtcg ccatctcacg cgagcacgag cggacgttct
     8701 cggcgtcggt gctctacgtc gcaatcggcg cggccggggc gctcgcgctg tcgctcctcg
     8761 acgtggaggg gctcgacccc gagggagatc acctcgtcat ggagcgactt tccgagctgg
     8821 cgctgatcgc ggcggtgttc tcggcgggcc tcacgatcga gcgacacgtg cagcgccggt
     8881 cgctcgtctc gctcgccacg ctgctcgtcg tcgtcatgcc gctgacgatc gccgccattg
     8941 ccctgttcgg catgtgggcg atgggcctct cgttcggtgc ggcgctgctg ctcggtgccg
     9001 tgctcgcgcc caccgatccc gtgctcgccg gcgacgtcgg gctatccgcg cccggcggtg
     9061 aggtgtacgg ggagccgcgc ctgtccctgc acaccgaggc gggcttcaac gacgggctcg
     9121 cctcgccgtt cgtggtgctc ggcctcttcg tcgccgagct cggcgggagc gactgggtgg
     9181 gtgagtggat cggcgccgac ctgctctacg gcgccggggt gggactcgcg ctcggcgcgg
     9241 cggccggctt cgccgcggcg gcggggctca cggccgcgcg cggcgccggt gtgatcaccg
     9301 gccggctcga gggcttcgtg accatcggac tgatgctcgc gatctacggg gcgagcgagg
     9361 cggtcggaac ctacggcctg ctggccgtct tcgcggccgg cttcacgttc cgccgctacg
     9421 agttcgacca cgagatgcac catcccgtcc accagggagc ccacgcggcg gggaaggcgc
     9481 tcgagctgat cgtcctgctg atgctcggca cgatgctcac caccgccggg ctcggcgcac
     9541 ctggggtcga gggctggctg ctcgtcccgg tcctgctgct cctgatccgc cccgccctgg
     9601 tgttcgccac cgcgggcagg cgcttcatgg acgtgcgcgg ccgcctgttc ctcgcgttct
     9661 tcggagtgcg tggggtcgcc gctctcttct acgccgcggt ggtggtcgag gcgggcgtgc
     9721 tcgaaccggg cgagcaacac gtggtggtgt ggacgacgat cgtgtgcgtg gtctgctcga
     9781 tcgtggtgca tggcgtgagc gccgcgccgc tcacccgccg ctggctcggc ggctagctgc
     9841 gggcgttctc ggcggtgaca tgggtgcttc gcggtcggcc tgacaagctt gccgcccgtg
     9901 cacgtggacg agtacatcct gatcgcctcc gcactgctgg ccggcggact cggcgcgtcg
     9961 ctgatggcgc ggcggctgcg gctgcccagc ctgctgctgt tcctggggct cggcatggcc
    10021 gtcgggtcgg acggggccgg gtggatcgag ttcaacgact acgagctcgc gcgcggcatc
    10081 ggcgtgatcg cgctgtcgct gattctgttc gagggcgggc tgagcgctgg cttcgccgag
    10141 atccggccgg tgctgcgccc cgcgatcgga ctagccctgc tgggcacgct ggcgaccgcg
    10201 gtgatcaccg gc
//
LOCUS       DASVPL010000075         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_9494, whole genome shotgun sequence.
ACCESSION   DASVPL010000075 DASVPL010000000
VERSION     DASVPL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9494"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VF517_03525"
     CDS             <1..432
                     /locus_tag="VF517_03525"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052036.1"
                     /translation="AAPGARLRAASDLRYSGQAFELTVPGAAAPEPAELRRAFDRAHE
                     ERYGYADPEAALELVTVRVAVALPATELPPEPRADQPRRGSRRAWFAGDHLEIAVLSG
                     PPDAALEGPAIVELDEATLVVPPGWRCGTAADGTIEMTWTR"
     gene            420..1913
                     /locus_tag="VF517_03530"
     CDS             420..1913
                     /locus_tag="VF517_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX8052037.1"
                     /translation="MDPVALQVMVGALRAACEEMGVVLVRSAHSANIKERRDASTALF
                     DARGEMAMQAEHIPVHLGAMPAAVEAVAAERPEPGDAWILNDPYRGGTHLPDITVVSP
                     VHADGELMGYAASRAHHADVGGKVPGSMPADSTTLDEEGVVIEPCRLVRGGELDRELL
                     DSITARMRGPRQRVADLRAQLAANRAGAARLVERTERHGLDAVREAMAETLDYAERRT
                     RAAIERLGDGEREARDVLEAPEGDLELRLRARVEGGSIVLDFTGSADQHGGNLNCPLA
                     VTLSACYFAVRVLADPDVPPCAGAYRPVEVIAPEGSVLNARPPAAVVAGNVETSSRVA
                     DLVLAAFGHALGQGTMNNLTLGNDGFTYYETIGGGQGACADADGPSAVHVAMSNTLNT
                     PVEALELEFPLRAVEYSLRRGSGGDGARRGGDGVVREVEALEEMQYSLLTERRRTRPA
                     GADGGSAGACGRNLVDGEELPPKAAGTLRPGQRLRIETPGGGGHGRT"
     gene            1900..>2561
                     /locus_tag="VF517_03535"
     CDS             1900..>2561
                     /locus_tag="VF517_03535"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8052038.1"
                     /translation="MDAPRRIAFLGMGIMGRPMASNLAAAGFEVTVWNRTASRARDTG
                     LPVADTPAEASAGADVTITMVVDSPQVEEVLFGENGGSEGMRDGHVAVDMSTIAPTAA
                     RSIGERLSVPFLDAPVTGSRPKAEDATLTIMAGGDEAAYERVRPVLEAMGKLVLRVGP
                     SGHGQMAKLINNTLAAIHAAALAEGLALAEGFGLDTDALRKVVGAGSGASAMLFLKAG
                     PML"
BASE COUNT          333 a          913 c          990 g          325 t
ORIGIN      
        1 gcggccccgg gcgcccggct gcgagccgcc tccgatctgc gctactccgg ccaggccttc
       61 gagctgacgg tgcccggggc ggcggcgccg gagcccgccg agctgcgccg agccttcgac
      121 cgcgcccacg aggagcgcta cgggtacgcc gatcccgagg cggcgctcga gctggtgacg
      181 gtgcgggtgg cggtcgcttt gccggcgacc gagctgccgc cggagccgcg tgccgaccag
      241 ccccggcgcg gcagccggcg cgcgtggttt gccggagatc acttggagat agcggtcttg
      301 tcaggaccac cggatgcggc actcgagggg cccgcgatcg tggagttgga cgaggccacg
      361 ctggtggtgc cgccgggctg gcgctgcggc acggccgcgg acggaacgat cgagatgacg
      421 tggacccggt agcgctccag gtgatggtcg gcgcgctgcg cgccgcgtgc gaggagatgg
      481 gcgtggtgct cgttcgctcg gcccactcgg cgaacatcaa ggagcgccgc gacgcatcca
      541 cggccctgtt cgacgcccgc ggcgagatgg ccatgcaggc cgagcacatc cctgtgcacc
      601 tcggtgcgat gccggccgcc gtcgaggccg tggccgccga gcggcccgag cccggcgacg
      661 cgtggatcct caacgatccc taccgcggcg gcacgcatct ccccgacatc acggtggtct
      721 cccccgtgca cgcggacggc gagctcatgg gctacgcggc cagccgcgcg caccacgccg
      781 acgtgggcgg gaaggtgccg ggctcgatgc ccgccgactc gaccacgctc gacgaggagg
      841 gcgtggtgat cgagccgtgc cggctggtgc gcggcggcga gctcgaccgc gagctgctcg
      901 actcgatcac cgcgcgcatg cgcggcccgc ggcagcgcgt tgccgatctg cgcgctcagc
      961 tcgccgccaa ccgcgccggc gccgcgcggc tggtcgagcg gaccgagcgc cacggcctcg
     1021 acgcggtccg cgaggcgatg gccgagacgc tcgactacgc cgagcgccgc acgcgcgccg
     1081 cgatcgagcg cctcggcgac ggcgagcgtg aggcgcgcga cgtgctcgag gcgcccgagg
     1141 gcgacctcga gctgcgcctg cgcgcgcgtg tggagggcgg ctcgatcgtg ctcgacttca
     1201 cgggctcggc cgatcagcac ggcgggaacc tcaactgccc gctggcggtc acgctgtcgg
     1261 cctgttactt cgccgtgcgg gtgctggccg atcccgacgt tcccccgtgc gcgggcgcgt
     1321 accgcccggt cgaggtgatc gcgccggagg gctccgtgct gaacgcccgc ccaccggcgg
     1381 ccgtggtcgc gggcaacgtg gagacctcgt cacgcgtggc cgacctcgtg ctggcggcct
     1441 tcgggcacgc gctcggccag gggacgatga acaacctcac cctcggcaac gacgggttca
     1501 cctactacga gacgatcggc ggtggtcagg gcgcgtgcgc ggacgccgac gggccgagcg
     1561 ccgtgcacgt ggcgatgagc aacacgctca acacgccggt ggaggcgctg gagctcgagt
     1621 tcccgctgcg ggcggtggag tactcgctgc ggcgcggctc gggcggggac ggcgcacggc
     1681 gcggtggcga cggcgtggtg cgcgaggtcg aggcgctcga ggagatgcag tactcgctcc
     1741 tcaccgagcg ccggcggacg cggccggccg gggcggacgg cggctcggcg ggagcctgcg
     1801 ggcgcaacct cgtggacggc gaggagctgc cgcccaaggc cgcgggtacg ctgcgccccg
     1861 gccagcgatt gcgcatcgag acccccggag gtggaggaca tggacgcacc tagacggatc
     1921 gccttccttg gcatggggat catgggcagg ccgatggcga gcaacctcgc cgccgcgggg
     1981 ttcgaggtca cggtgtggaa ccgcaccgcg tctcgggctc gcgacacggg tctccccgtc
     2041 gcggacaccc cggccgaggc gagtgcgggc gccgatgtga cgatcacgat ggtcgtcgac
     2101 tctcctcagg tcgaggaggt cctcttcggt gagaacggcg gcagtgaggg catgcgggac
     2161 gggcacgtcg cggtggacat gtcgacgatc gcgcccaccg ccgcacgctc catcggcgag
     2221 cggctctccg ttcccttcct cgacgccccc gtgaccggct cgcgccccaa ggccgaggac
     2281 gcgacgctca cgatcatggc cggcggcgac gaggccgcgt acgagcgcgt gcgcccggtg
     2341 ctcgaggcga tggggaagct cgtgctgcgg gtcgggccgt ccggccacgg tcagatggcc
     2401 aagctgatca acaacacgct ggcggccatc cacgccgccg cgctggccga gggcctcgcg
     2461 ctggccgagg gcttcggcct cgacacggac gcgctgcgca aggtcgtggg cgccggctcc
     2521 ggcgcgtcgg cgatgctctt cctcaaggcg ggccccatgc t
//
LOCUS       DASVPL010000076         6535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_166140, whole genome shotgun sequence.
ACCESSION   DASVPL010000076 DASVPL010000000
VERSION     DASVPL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166140"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="VF517_03540"
     CDS             complement(<1..1803)
                     /locus_tag="VF517_03540"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX8052039.1"
                     /translation="MSVTVGVNERLQALTAAGTSVWLDQIRRSLIEGGELKRLMDEMS
                     LRGVTSNPAIFEKAILGSDDYDDELRELANQGCDAKQIYDAIAIKDVQLAADVLRPVY
                     DELRGYDGYVSLEVGPEAAHDTEATLEQARDYWKRVDRPNVMIKIPGTTAGLPAIEEC
                     ISEGINVNVTLLFSVESYANVADAYVRGLERLRENGGDVTNVRSVASFFVSRVDSKVD
                     KLLEARGNTELQGAAGLWNARAAYVRFKEIFHGDRFAELREAGAPVQRPLWASTGVKN
                     PNYSETMYVDGLVAPETVNTMPMPTLLAAGEKAEISGATADADPAEVEAAMKDLEDAG
                     IDMEQVVEELLDEGVTLFVDAMEKLIIGVESAREAAVTGRPATFESNIPDELEPAIAA
                     RVKQATEEDVARRVWRRDESLWGGPGPEIGDRLGWLTISDTMLEHCADLKAFAQACRE
                     DGLTDAVLLGMGGSSLGPEVIRRSFGDGTDGMRLHVLDSTDPGAVLEAERAIDLKSAL
                     FIVSSKSGGTIETLSQFRYFHARASEALGAEEAGKHFVAITDPDTSLAELAREKGFLH
                     TFVNDPEIGGRYSVLSYFGLVPAALMGVDVSAVLE"
     gene            complement(1805..3832)
                     /gene="tkt"
                     /locus_tag="VF517_03545"
     CDS             complement(1805..3832)
                     /gene="tkt"
                     /locus_tag="VF517_03545"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX8052040.1"
                     /translation="MTTTATDTRALDELCVNTIRTLSMDAVQKANSGHPGAPMGLAPL
                     AYVLYTRVMRHNPANADWPNRDRFVLSAGHASMLLYSMLHLTGYEMSLDDIKNFRQVG
                     SPTAGHPERKYSPGIESTTGPLGQGIATAVGLALGERMLAAQFNEGEHEIVNHHTFTI
                     ASDGDMQEGVAAEASSLAGHLGLGKLIAFYDDNKIQLASPVDVVMTEDVGKRYEAYGW
                     HVIDVGDTDDLDRIETATREAMLVEDRPSLVIVQTHIGYGSPNKQDSTKAHGSPLGED
                     EIRLTKEAYGWDPDKQFFVPDEALEHFRESCARGSKVESAWKALFDRYREDHPEKAAL
                     FEAIQRGEMPEGFEEGLPRFDADPKGMATRAASGKVIQWAAEKVPHLVGGSADLASSN
                     NTNIDGGGDVATGDFGGRNIHFGVREHGMGAIVNGLGLQGLRAYGGTFLVFSDYMRGA
                     VRLSALMKLPSIWIWTHDSIGLGEDGPTHQAVEHLCALRAIPRLNVLRPADANETALC
                     WRFALRNTEAPSAFALSRQGLPVIDPEKIPDDAVERGAYVLHETGEGEPDLILIGTGS
                     EVSLCMEAAELLDGIAVRVVSMPCMDTFTEQDESYRESVLPRSVPARVAVEAASPMGW
                     DRWIGPDGAFLGMTTFGESGPAPAVYEHFGITAEKAAELGRSVLDRVGARS"
     gene            complement(3829..4815)
                     /locus_tag="VF517_03550"
     CDS             complement(3829..4815)
                     /locus_tag="VF517_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8052041.1"
                     /translation="MARGTEPARGAAEPAVIGVDVGGTKVAAARIAGTEVIDPVVHPT
                     DLSGSDALLAGIESAIREVGERSGAPEAVGIGMPSQIDFATGTIVSSVNIPFEGVALG
                     EELTARIGVPVYLDNDANCAALAEAQFVEDAPAQHLVMLTLGTGVGGGVVIGGRIFRG
                     ASGLGAELGHTVIDPDGPECPGSCPNRGCLEALASGTALEREATARAGGERVKGKQVV
                     EAARNGDAGAQELLRDLARWLGIGISNFVNVFEPAHFVIGGGLSAAADLFLSDAVEEA
                     QSRALPALFERVRVSVAKAGNDAGVIGAGLLAAQELSLKGDTAGLASDGRSA"
     gene            4963..5193
                     /locus_tag="VF517_03555"
     CDS             4963..5193
                     /locus_tag="VF517_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEX8052042.1"
                     /translation="MADQTDGQVLAPPSEEIHLPDPAYTPVVLAFGITLMIVGVVLTW
                     VMVAIGAVIALVALIRWIRQVREEMAELPLEH"
     gene            5195..5461
                     /locus_tag="VF517_03560"
     CDS             5195..5461
                     /locus_tag="VF517_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052043.1"
                     /translation="MLGPNVRWTLQRIALLDAPLLMIAAVASLFTDRPLLGIALALGS
                     FGMFMLGRPTIGRRYPPAEEERRGETPLATPVAKPRRRRDPRGF"
     gene            complement(5458..5853)
                     /locus_tag="VF517_03565"
     CDS             complement(5458..5853)
                     /locus_tag="VF517_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8052044.1"
                     /translation="MSSVVLTVGPDHTLREAAKQMAKRSVGAAVVIDPEEQGPGIVTE
                     RDLLNSLGAGEDPDTERVCDHLSENLTFAGPDWSLERAAEEMVRGGFRHLVVIDGGEI
                     AGVLSMRDIVRCWTEAGSTTEVETARTRA"
     gene            6122..6487
                     /locus_tag="VF517_03570"
     CDS             6122..6487
                     /locus_tag="VF517_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8052045.1"
                     /translation="MEIIEQEYASDLELDAVARRLATSRRQLQRAFAEAGRTSFRTRL
                     AEVRMQKALELLREGTLPVRDVATSVGYRQAAQFAKTFRRHHGRPPSSIRRERNAGAP
                     GDPGQPHTMATLDQPAAAA"
BASE COUNT          887 a         2438 c         2182 g         1028 t
ORIGIN      
        1 ctcgaggaca gcggacacgt ccacccccat cagcgccgcc gggaccagcc cgaagtacga
       61 gagcacgctg tagcgcccgc cgatctccgg gtcgttcacg aacgtgtgca ggaagccctt
      121 ctcgcgcgcg agctccgcca gcgacgtgtc cgggtcggtg atcgccacga agtgcttgcc
      181 ggcctcctcc gcgcccagcg cctcgctcgc gcgggcgtgg aagtacctga actgcgacag
      241 cgtctcgatc gtgccgcccg acttgctcga cacgatgaac agcgccgact tcaggtcgat
      301 ggcgcgctcg gcctcgagca cggcgccggg gtcggtcgag tcgagcacgt gcaggcgcat
      361 gccgtcggtg ccgtcgccga acgagcggcg gatcacctcg ggcccgaggc tcgagccgcc
      421 catgccgagc agcacggcgt cggtcagccc gtcctcgcgg cacgcctgcg cgaacgcctt
      481 caggtcggcg cagtgctcga gcatcgtgtc cgagatcgtg agccagccga gccggtcccc
      541 gatctcgggc ccgggcccgc cccagagcga ctcgtcccgg cgccacacgc gcctggccac
      601 gtcctcctcg gtggcctgct tcacgcgcgc cgcgatggcc ggctcgagct cgtcggggat
      661 gttgctctcg aacgtggcag ggcggcccgt gacggcggcc tcgcgcgcgc tctccacgcc
      721 gatgatcagc ttctccatcg cgtccacgaa cagcgtcaca ccctcgtcga gcagctcctc
      781 cacgacctgc tccatgtcga tccccgcgtc ctcgaggtcc ttcatggcgg cctcgacctc
      841 ggcggggtcg gcgtcggccg tggccccgct gatctcggcc ttctcgccgg cggcgagcag
      901 cgtgggcatc ggcatcgtgt tcaccgtctc gggcgccacc aggccgtcca cgtacatcgt
      961 ctccgagtag ttcgggttct tcacgccggt ggacgcccag agcgggcgct gcacgggcgc
     1021 gccggcctcg cgcagctcgg cgaagcggtc gccgtggaag atctccttga agcgcacgta
     1081 ggcggcccgc gcgttccaga ggccggcggc gccctggagc tcggtgttgc ccctggcctc
     1141 gagcagcttg tcgaccttgc tgtccacgcg cgagacgaag aagctcgcca ccgagcggac
     1201 gttcgtcacg tcgccgccgt tctcgcgcag gcgctccagc ccgcggacgt acgcgtcggc
     1261 cacgttcgcg tacgactcga cgctgaacag cagcgtcacg ttcacgttga tgccctcgga
     1321 gatgcactcc tcgatcgccg gcaggccggc ggtcgtgccc gggatcttga tcatcacgtt
     1381 cgggcggtcg acgcgcttcc agtagtcgcg cgcctgctcg agcgtggcct cggtgtcgtg
     1441 ggccgcctcg ggccccacct cgagcgagac gtagccgtcg tagccgcgca gctcgtcgta
     1501 gaccgggcgc agcacatcgg cggcgagctg cacgtccttg atcgcgatcg cgtcgtagat
     1561 ctgcttcgcg tcgcagccct ggttcgcaag ctcgcgcagc tcgtcgtcgt agtcgtcgga
     1621 cccgaggatc gccttctcga agatcgccgg gttcgaggtc acgccgcgca gcgacatctc
     1681 gtccatcagc cgcttcagct cgccgccctc gatcaggctg cggcggatct ggtcgagcca
     1741 gacgctcgtc ccagccgccg tcagggcctg gagccgctcg ttcactccga cagtcacgct
     1801 catctcagct cctcgctcct acgcggtcaa gaacgctccg acccagttcc gccgccttct
     1861 ccgccgttat cccgaaatgc tcgtagaccg cgggggccgg ccccgactcg ccgaacgtgg
     1921 tcatgcccag gaaggccccg tcagggccga tccacctgtc ccagcccatc gggctcgcgg
     1981 cctcgaccgc cacccgcgcg ggcaccgagc gcggcagcac cgactcgcgg taggactcgt
     2041 cctgctcggt gaaggtgtcc atgcacggca tcgacacgac gcgcacagcg atgccgtcga
     2101 gcagctcggc ggcctccatg cacagggaca cctcggagcc ggtcccgatc aggatcaggt
     2161 cgggctcgcc ctcgcctgtc tcgtgcagca cgtaggcgcc gcgctcgacg gcgtcgtcgg
     2221 gaatcttctc cgggtcgatc acgggcagcc cctggcgcga gagcgcgaag gcgctcggag
     2281 cctccgtgtt gcgcagggcg aagcgccagc acagggcggt ctcgttcgcg tccgccgggc
     2341 gcagcacgtt cagccgcggg atcgcgcgca gggcgcagag gtgctcgacc gcctggtggg
     2401 tcggaccgtc ctcgccgagc ccgatcgagt cgtgcgtcca gatccagatc gacggcagct
     2461 tcatcagcgc cgacagccgc acggcgccgc gcatgtagtc gctgaacacg aggaacgtgc
     2521 cgccgtaggc gcgcagcccc tggaggccga gcccgttgac gatcgcgccc atgccgtgct
     2581 cgcgcacgcc gaagtggatg ttgcgcccgc cgaagtcgcc ggtcgcgacg tcgccgccgc
     2641 cgtcgatgtt cgtgttgttc gagctggcga ggtcggccga gccgccgacg aggtggggaa
     2701 ccttctcggc cgcccactgg atgaccttgc ccgacgcggc gcgggtggcc atccccttcg
     2761 ggtcggcgtc gaagcgcggc agcccctcct cgaagccctc gggcatctcg ccgcgctgga
     2821 tcgcctcgaa cagcgccgcc ttctcgggat ggtcctcgcg gtagcggtcg aacagcgcct
     2881 tccaggcgct ctcgaccttc gacccgcgcg cgcacgactc gcggaagtgc tcgagcgcct
     2941 cgtccgggac gaagaactgc ttgtcgggat cccagccgta cgcctccttg gtcagccgga
     3001 tctcgtcctc gccgagcggc gacccgtggg ccttggtgct gtcctgcttg ttcgggctgc
     3061 cgtagccgat gtgggtctgc acgatcacga gcgacggcct gtcctcgacc agcatcgcct
     3121 cgcgcgtggc ggtctcgatg cggtcgaggt cgtcggtgtc ccccacgtcg atcacgtgcc
     3181 agccgtaggc ctcgtagcgc ttgcccacgt cctcggtcat caccacgtcg accgggctcg
     3241 cgagctggat cttgttgtcg tcgtagaacg cgatcagctt gcccagtccg aggtggcccg
     3301 ccagcgacga cgcctcggcg gccacgccct cctgcatgtc gccgtcgctc gcgatcgtga
     3361 acgtgtggtg gttcacgatc tcgtgctcgc cctcgttgaa ctgcgcggcc agcattcgct
     3421 cgccgagcgc gagcccgacg gccgtggcga tcccctggcc gagcgggccg gtggtggact
     3481 cgatccccgg cgagtacttg cgctcgggat ggccggcggt cggcgagccg acctggcgga
     3541 agttcttgat gtcgtcgagc gacatctcgt agcccgtgag gtggagcatc gagtagagga
     3601 gcatcgacgc gtgccccgcg ctcagcacga agcggtcgcg gttcggccag tcggcattcg
     3661 ccgggttgtg gcgcataacg cgcgtgtaga gcacgtacgc cagcggagcg aggcccatcg
     3721 gggcgccggg gtggccggag ttcgccttct ggacggcgtc catcgagagc gttcggatgg
     3781 tgttgacgca gagctcgtcg agcgctctcg tgtcggtcgc cgtcgtggtc atgcgctcct
     3841 cccgtcgctt gccaggcccg cagtatctcc tttgagcgac agctcctgag ctgccagcag
     3901 gccggcacct attaccccgg cgtcgttgcc cgccttcgca acgcttaccc ggacgcgctc
     3961 gaacaacgcg gggagggccc ggctttgcgc ctcttcgacc gcatcgctca ggaagaggtc
     4021 ggccgccgcc gagaggccgc cgccgatcac gaagtgggcc ggctcgaaga cgttcacgaa
     4081 gttcgagatg ccgatgccga gccagcgggc gaggtcgcgc agcagctcct gcgcgccggc
     4141 atcgccgttg cgcgcggcct cgaccacctg ctttcccttc acccgctcgc cgccggcccg
     4201 cgccgtggcc tcccgctcca gcgcggtccc cgacgccagc gcttccagac accctctgtt
     4261 gggacacgat cccgggcatt cgggcccgtc ggggtcgatc accgtgtggc ccagctccgc
     4321 gccgaggccg ctggcgccgc ggaagatccg accgccgatc accacgccgc cccccacgcc
     4381 ggtgccgagc gtgagcatca cgaggtgctg ggccggcgcg tcctcgacga actgggcctc
     4441 ggcgagcgcg gcgcagttgg cgtcgttgtc gaggtagacc ggcacgccga tgcgggcggt
     4501 cagctcctcg cccagggcca cgccctcgaa cgggatgttc acgctgctca cgatcgtgcc
     4561 ggtggcgaag tcgatctgcg acggcatccc gatcccgacg gcctcgggcg cgccggagcg
     4621 ctcgccgacc tcgcggatgg cggattcgat cccggccagg agggcgtcgc tgccgctcag
     4681 gtcggtcggg tggaccaccg gatcgatcac ctcggtgccc gcgatccgcg cggcggcgac
     4741 cttcgtgccg cccacgtcca cgccgatcac cgcgggctca gccgctcccc gcgccggctc
     4801 tgtcccccgg gccacgccgg aaacctacgc cctttgtgct agaaacgatc cctcgatgga
     4861 ccggcgactc gccaacaaga acctgcgtac cggcctcatc gccggcgcga tcagcttcgt
     4921 cttcttcggc gcggcgttcc tggtcgccgc cgcgtactag cgatggccga ccagaccgac
     4981 ggccaggtgc tcgctccgcc gagcgaggag atccaccttc ccgatcccgc ctacaccccc
     5041 gtggtgctgg cgttcggcat cacgctcatg atcgtgggcg tcgtcctcac ctgggtgatg
     5101 gtcgcgatcg gcgccgtcat cgccctcgtg gcgctcatcc gctggatccg ccaggtccgc
     5161 gaggagatgg cggagctccc gctcgagcac tagcgtgctc ggcccgaacg tccgctggac
     5221 cctccagcgc atcgcgctgc tcgacgcgcc cctgctgatg atcgccgcgg tggcgagcct
     5281 gttcaccgac cggccgctgc tcgggatcgc cctcgcgctc ggctcgttcg gcatgttcat
     5341 gctcggccgg ccgacgatcg gccgccgcta cccgccggcg gaggaggagc gccgcggcga
     5401 gacgccgctc gccacgccgg tggccaagcc gcgccgccgg cgcgacccgc gcgggttcta
     5461 agcgcgcgta cgcgccgtct cgacctcggt ggtcgagccg gcctcggtcc agcagcgcac
     5521 gatgtcgcgc atcgacagga cgccggcgat ctcgccgccg tcgatcacca cgaggtgacg
     5581 gaagccgccc ctgaccatct cctcggccgc gcgttcgagc gaccaatccg gccccgcgaa
     5641 ggtgaggttc tcggagaggt ggtcgcagac gcgctcggtg tcggggtcct cgcccgcgcc
     5701 cagggagttc aggaggtcgc gctcggtgac gatcccgggt ccctgctcct cggggtcgat
     5761 caccacggcg gcgccgacgc tacgcttcgc catctgcttc gccgcctcgc gcagggtgtg
     5821 gtccgggccg accgtgagaa ctaccgagct catcccctcg ctgactcgca aatccactcc
     5881 cctgtcgatc aagtgacgat tgccgcgagc ttaccgcccg cgccctgacg atgcgtcgcg
     5941 agtgtgcgtg cgccaagccc gtgcaggaaa ggtgacccaa gcgtcatatt cgctaacttg
     6001 gccgctgatc cgcccgttgg tgttggcacg tacccgacac gaccccggac tcgcaccact
     6061 tacccctatg cccattagaa catccacagt tgcgcgccgc cgtgccctct acgaggaagc
     6121 ggtggagatc atcgagcagg agtacgcgag cgacctcgag ctcgacgccg tcgcccgccg
     6181 tctggcgacg tcgcgacgcc agttgcagcg cgccttcgcc gaggcgggac ggaccagctt
     6241 ccgcacccgc ctggccgagg ttcgcatgca gaaggcgctc gagctgttgc gcgagggcac
     6301 gctgcccgtg cgcgacgtcg cgaccagcgt cggctaccgg caggcggccc agttcgcgaa
     6361 gacgttccgc cgccaccacg gccgcccgcc gtcgagcatc cgccgcgagc ggaacgccgg
     6421 ggcgccaggc gatccgggcc agccgcacac gatggctacg ctcgaccagc cggccgcagc
     6481 cgcgtagcgc ccggctcgaa agctaggccg gcacagccgg ccgcaggagc gcgcg
//
LOCUS       DASVPL010000077         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_89034, whole genome shotgun sequence.
ACCESSION   DASVPL010000077 DASVPL010000000
VERSION     DASVPL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89034"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VF517_03575"
     CDS             complement(<1..1261)
                     /locus_tag="VF517_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8052046.1"
                     /translation="MPRVRRPRTPAQIARLRRRIRRPPGHWTLLAFCLVTMLVLLGVQ
                     GIAAHTTERSSTRDAAGGGEPFGGAGPLLTLDRGRLVSREPPAGRAVALTFDDGPDPR
                     WTPRIAAELKRLGAPATFFVLGSKVVRHPDLVRDLHRDGFELGNHTFSHADVTGLPGW
                     ELSLQLGLTENAVAGASGVRPRLFRPPYSATPEAVTSAGARGLRRVAKHGYAIVLSDL
                     DTRDWSRPGVEAIVRAATPPSDAGGLLLMHDGGGNRSQTVAALRRIVPELRGRGFRFV
                     TASQLAGIDRAAAEPATSSWERGRGRLLLATLGVAHFVTDALTLMLVPIALLALLRMA
                     ALYFFARRHARIVRAAAAADRDEFLPPVSIVVPAFNEAAGIERAVRSLDASDYPDFEV
                     LVVDDGSEDGTGDIVETLELDHVRVLRQ"
     gene            complement(1270..1929)
                     /locus_tag="VF517_03580"
     CDS             complement(1270..1929)
                     /locus_tag="VF517_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052047.1"
                     /translation="MERTEAPGLPAPQREDERPVFASETQRRARVLRVLGWTLASATA
                     LWLVALVLGAFGLHPLPSLGLPDVPGGSGATAQTDRGAGDQAVRNVERAGADRDATRL
                     AAARDEAARDDAAGSARGERSARRGSGDREAPATETGGDDSAPAGTGSGGSGTTTPTA
                     PAAPPTTAPAAGGNSATAPGAVNRPDRTTDAPRGNSGSAPGATTSGERRGGPKADTTT
                     P"
     gene            2035..3120
                     /gene="gcvT"
                     /locus_tag="VF517_03585"
     CDS             2035..3120
                     /gene="gcvT"
                     /locus_tag="VF517_03585"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX8052048.1"
                     /translation="MAAVAETLRRTPLHDRHAAAGARLVPFAGWEMPVQYEGIRQEHV
                     AVRTSAGIFDVSHMGEVETRGPDAEAFLQRILSNDVTKIPVGGAQYSVLCKENGGVLD
                     DLFTYRLGDDRYLTVTNASNHERDHDWIAKHAADFDVTVTDAAADWSMIALQGPNARA
                     ILNDLIEGEAPARMRASEARVANVPALVCGTGYTGEDGVELLVAPDDAETVWDALAAA
                     GATPAGLGARDTLRLEVCFHLYGNDLAEDRNPIEAGLDWVCKLDTGFVGSDVLRTVRP
                     EQRIVPFAFTGPGIPRQGNPIHTEHGDGVVTSGTMSPCLEIGIGMGYVPLAAGEPGTP
                     IEVDVRGKKRPAEVRERPLYRREAASG"
     gene            3113..3505
                     /gene="gcvH"
                     /locus_tag="VF517_03590"
     CDS             3113..3505
                     /gene="gcvH"
                     /locus_tag="VF517_03590"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX8052049.1"
                     /translation="MAESYPDDLKYHPEHDWARIDGEEATFGITWFAQDQLGEVVFFD
                     PPTVGDTVTANESYAEVESVKAVSDVVAPLSGEVLAVNEELAEAPEKVNDDPYGDGWL
                     VRVKLTDPSQVDGLLDVAAYQELVKGAS"
     gene            3502..>4359
                     /gene="gcvPA"
                     /locus_tag="VF517_03595"
     CDS             3502..>4359
                     /gene="gcvPA"
                     /locus_tag="VF517_03595"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX8052050.1"
                     /translation="MTRYTSATDDDRRAMLERIGAGSVEELFEDVPASVRLDRPLDLP
                     QGMSEQDVFDHLSALAERNRHADSEVTFAGAGMYDHYQPALIDSIISRSEFLTPYTPY
                     QPEISQGGLQVMFEYQTAISELTGLPVSNASLYEGPSSVASAGYLAKLENKRTRFVTS
                     RGLHPHSRAALATYARGFGATVEETPLDENGATDIEALAAAVDDDTAAVFVQQPNFLG
                     TVEDLGPLVEAGKRTGALVVCQVDAMTLGILKPPGEYGVDIAVGEGQPLGNRLDYGGP
                     SFGFFAAQER"
BASE COUNT          603 a         1597 c         1627 g          532 t
ORIGIN      
        1 cctggcgcag cacccgcacg tgatcgagct cgagcgtctc gacgatgtcg ccggtgccgt
       61 cctcggagcc gtcgtcgacc accagcacct cgaagtccgg gtagtcgctc gcgtcgagcg
      121 agcgcaccgc ccgctcgatc cccgccgcct cgttgaaggc cgggacgacg atcgataccg
      181 gcggcaggaa ctcgtcgcgg tccgcggcgg cggccgcgcg cacgatccgc gcgtggcggc
      241 gcgcgaagaa gtacaacgcg gccatgcgca gcagcgcgag cagcgcgatc gggacgagca
      301 tgagcgtgag cgcgtccgtg acgaagtgcg caacgccgag cgtggccagc agcaggcgcc
      361 cgcggccgcg ctcccacgac gaggtggccg gctcggcggc ggcgcggtcg atcccggcca
      421 gctgcgaggc ggtcacgaag cggaagccgc gcccgcgcag ctccggcacg atgcgccgca
      481 gcgccgcgac ggtctgcgag cggttgcccc caccgtcgtg catcagcagc aggccgcccg
      541 cgtccgaggg cggggtggcg gcgcgcacga tcgcctccac ccccgggcgc gaccagtcgc
      601 gcgtgtcgag gtccgacagc acgatcgcgt agccgtgctt cgccacgcgg cgcagcccgc
      661 gcgcgccggc gctggtcacc gcctccggcg tggccgagta gggcggacgg aacagtcgcg
      721 ggcgcacgcc gctcgcgccc gcgacagcgt tctcggtgag gccgagctgc agcgacagct
      781 cccagccggg caggccggtc acgtcggcgt ggctgaacgt gtggttgccg agctcgaagc
      841 cgtcccggtg gaggtcgcgc acgagatccg ggtggcgcac caccttgctg ccgagcacga
      901 agaacgtcgc gggggcgccc aggcgcttga gctcagccgc gatgcgcggc gtccagcgtg
      961 ggtcggggcc gtcgtcgaag gtgagcgcga ccgcccgccc ggccggcggc tcccgcgaca
     1021 ccagccgccc gcggtccagc gtcagcagcg gacccgcccc gccgaagggc tcaccgccgc
     1081 ccgcggcgtc gcgcgtggag ctgcgctcgg tggtgtgcgc ggcgatcccc tgcacgccga
     1141 gcagcacgag catcgtcacc aggcagaacg ccagcagcgt ccagtggccg ggcgggcgcc
     1201 ggatgcgccg ccgcaggcgc gcgatctggg ccggggtgcg gggtcggcgg acccgtggca
     1261 cgccggacct cagggagtgg tcgtgtcggc cttgggaccg ccgcggcgct cgccggaggt
     1321 ggtggcgccg ggggccgagc cggagttgcc gcgcggggcg tccgtcgtgc ggtcgggacg
     1381 gttgaccgct ccgggcgcgg tggccgagtt gccgccggcg gcgggggcgg tcgtgggcgg
     1441 cgcggcgggc gcggtgggag tggtcgtgcc cgagccgccg ctgccggtgc cggctggagc
     1501 cgagtcatcg ccgcccgtct ctgtggccgg cgcctcgcgg tcccccgagc cgcggcgggc
     1561 cgagcgctcg ccgcgggccg agcccgccgc atcgtcgcgc gcggcctcgt cgcgcgcggc
     1621 ggccaggcgc gtggcgtcac ggtccgctcc ggcgcgctcg acattgcgga ccgcctggtc
     1681 gccggcgccg cggtcggtct gcgcggtcgc tccgctgccg cccggcacgt cgggcaggcc
     1741 gagcgagggg agcggatgca gtccgaacgc gccgagcact agcgccacca gccacagcgc
     1801 ggtggcggag gcgagcgtcc agccgagcac gcgcagcacg cgggcgcggc gctgggtctc
     1861 ggaggcgaac acgggccgct cgtcctcgcg ctggggcgcg ggcaggccgg gcgcttcggt
     1921 cctctccacg gcgggcatcg agatctcaag cgtagaaacg cgctctcacc ccgaaagttg
     1981 gacccgtgtc cccggtgcgg gtcagccggg gtgcgtcggt agagtctcga cggcttggcc
     2041 gccgtcgccg aaacgcttcg ccgcaccccg ctccacgacc gtcacgccgc ggccggggcg
     2101 cgcctcgtgc cgttcgccgg ctgggagatg ccggtgcagt acgagggcat ccggcaggag
     2161 cacgtggcgg tccggacgag cgccggcatc ttcgacgtct cgcacatggg cgaggtcgag
     2221 acgcgcgggc ccgacgcgga ggccttcctc cagcgcatcc tctcgaacga cgtcacgaag
     2281 atccccgtcg gcggagccca gtactcggtg ctctgcaagg agaacggagg ggtgctcgac
     2341 gacctcttca cctatcgcct cggcgacgac cgctacctga ccgtcacgaa cgcgagcaac
     2401 cacgagcgcg accacgactg gatcgccaag cacgccgccg atttcgacgt gaccgtgacc
     2461 gacgcggccg ccgactggtc gatgatcgcc ctccaggggc cgaacgcgcg cgcgatcctg
     2521 aacgacctga tcgagggtga ggcgcccgcg cgcatgcgcg cgagcgaggc ccgtgtggcg
     2581 aatgtgcccg cgctcgtctg tggcaccggc tacacgggcg aggacggcgt cgagctgctg
     2641 gtcgcgccgg acgacgccga aacggtctgg gacgccctgg ccgcggccgg cgccaccccc
     2701 gccggcctgg gcgcccgcga cacgctgcgc ctggaggtct gcttccacct ctacggcaac
     2761 gacctcgccg aggaccgcaa cccgatcgag gccgggctcg actgggtctg caagctcgac
     2821 accggcttcg tcggctcgga cgtgctgcgc acggtcaggc ccgagcagcg gatcgtcccg
     2881 ttcgccttca cgggcccggg catcccgcgc cagggcaacc cgatccacac cgagcacggc
     2941 gacggcgtcg tgaccagcgg gacgatgtcc ccgtgcctgg agatcgggat cggcatgggc
     3001 tacgtaccgc tggcggcggg cgagccgggc acgccgatcg aggtggacgt ccgcggcaag
     3061 aagcgccccg ccgaggtgcg cgagcggccg ctctacagac gggaggcggc gagtggctga
     3121 gtcgtatccg gatgacctga agtaccaccc cgagcacgac tgggctcgga tcgacggcga
     3181 ggaggcgacc ttcgggatca cctggttcgc gcaggaccag ctcggcgagg tcgtgttctt
     3241 cgaccccccc acggtcggcg acaccgtgac cgccaacgag tcgtacgcgg aggtcgagtc
     3301 cgtgaaggcg gtatccgacg tggtcgcgcc gctgagcggc gaggtcctgg ccgtgaacga
     3361 ggagctggcc gaggcgcccg agaaggtcaa cgacgacccc tacggcgacg gctggctcgt
     3421 gcgcgtgaag ctcaccgacc cgtcgcaggt ggacggcctg ctcgacgtgg ccgcctacca
     3481 ggagctcgtg aagggagcct cgtgacgcgc tacaccagcg cgaccgacga cgaccgccgc
     3541 gcgatgctcg agcgcatcgg cgcgggctcg gtcgaggagc tgttcgagga cgtgccggcc
     3601 tcggtgcgcc tcgaccgccc gctcgacctg ccgcagggca tgtccgagca ggacgtgttc
     3661 gaccacctga gcgcgctcgc cgagcgcaac cgccacgccg actcggaggt caccttcgcc
     3721 ggcgccggga tgtacgacca ctaccagccg gcgctgatcg actcgatcat ctcgcgcagc
     3781 gagttcctca ccccgtacac gccgtaccag ccggagatct cccagggcgg gctccaggtg
     3841 atgttcgagt accagaccgc gatctccgag ctcaccggcc tgccggtctc gaacgcctcg
     3901 ctgtacgagg gaccgtcgag cgtggcgagc gccgggtacc tggcgaagct cgagaacaag
     3961 cgcacgaggt tcgtcacctc gcgcggcctg cacccgcact cgcgcgctgc gctcgccacc
     4021 tacgcgcgcg gcttcggcgc caccgtcgag gagacgccgc tcgacgagaa cggcgccacc
     4081 gacatcgagg cgctagccgc cgccgtcgac gacgacaccg ccgccgtgtt cgtccagcag
     4141 ccgaacttcc tcggcacggt cgaggacctg ggcccgctgg tcgaggccgg caagcgcacc
     4201 ggcgcgctgg tcgtctgcca ggtcgacgcg atgacgctcg gcatcctcaa gccgcccggc
     4261 gagtacggcg tcgacattgc ggtcggtgag gggcagccgc tcggcaaccg gctcgactac
     4321 ggcggtccgt cgttcggctt cttcgccgcg caggagcgc
//
LOCUS       DASVPL010000078         5338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_204762, whole genome shotgun sequence.
ACCESSION   DASVPL010000078 DASVPL010000000
VERSION     DASVPL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204762"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1954)
                     /gene="lon"
                     /locus_tag="VF517_03600"
     CDS             complement(<1..1954)
                     /gene="lon"
                     /locus_tag="VF517_03600"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX8052051.1"
                     /translation="MDDTVVFPTMNLTLALEVGDEERVVLVPRHEDAFASVGTVADVV
                     ERVRLPGGGRAVSLVGLHRGRIGAAQTGPSGDLRVEVEPHPDDEPVDGRTRELAREYR
                     AVVEEILELRGDDGRIQAFLRSISEPGALADTAGYSPDISYEQKIELLETLDVTERLE
                     KSLAFQRDRLTELEVRARIREDVRSGTENQQREYFLRKQMESIRKELGDDDGSVVEDY
                     RTQIEEAGMPDEVREQAEKELARLERSGEQSGESSMIRSYLDWLIAVPWSERSDERLD
                     PVHARKVLDADHAGLDEVKDRITEYIAVRRLRKERGIAEDRKSGAILTLIGPPGTGKT
                     SIGESIARATGREFVRMSLGGVRDEAEIRGHRRTYIGALPGRLVRALRDARTMNPVIM
                     LDEVDKVGADWRGDPSAALLEVLDPAQNHSFRDHYLDVELDLSEVVFIATANVAETIP
                     GPLLDRMEIIRFDGYTTAEKLAIARGYLWPRQRERNGLREDEVTVSDDVLRTVVTEYT
                     REAGVRQLERELGTVLRKTATRIASGSAEAPVAIEADAVRDALGRQRFFQEAAERTAI
                     PGVATGLAVTGTGGDVLFVEATGMKGKNDLVLTGQLGDVMKESARIALSYVRGHAERL
                     DVDEDAFEDREFHVHVPAGAIPKDGPS"
     gene            2024..2980
                     /locus_tag="VF517_03605"
     CDS             2024..2980
                     /locus_tag="VF517_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8052052.1"
                     /translation="MGSLAGMKLEGIHHITCITGDAPRNVDFYARVMGLRLVKKTVNQ
                     DDPTIYHLFYGDEEGAPGHDLTFFEYPGAQPGKAGAGMVHRLIHRVGSADTLDFWAER
                     LGAEEVATRRENGSLVFADPEGLEHELAVFEVPDPPLIAHASEVPDEHALQGFEAVRT
                     YTADPEASRRLLEEGLGFEQRSGDEWEARGGERGGRMVYDRSQVRGVQGAGTVHHVAW
                     SVGLDELDAWRERVLAAGARPTPLIDRFWFHSIYFREPSGVLFELATRGPGFTADEPL
                     DTLGEALTLPPDYEQYRERLEQVLTPLPDPRQWRRAGSPQSG"
     gene            2977..3258
                     /locus_tag="VF517_03610"
     CDS             2977..3258
                     /locus_tag="VF517_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052053.1"
                     /translation="MTNEAKLLVFVRYVLPALIVVAGLATILIGDSKSALHGGLGIVG
                     GGIAVLLLSYFYRVGASGELERDAEVEARDYFDRHGRWPDEDQQLPGKS"
     gene            complement(3241..3783)
                     /locus_tag="VF517_03615"
     CDS             complement(3241..3783)
                     /locus_tag="VF517_03615"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8052054.1"
                     /translation="MSLEPVPEAELRTTEDGVIPASEGWFVVNVADAKAISSDRFGSS
                     ARFEDRVQASFPEFGINVRVLEPGQPSALYHRENAQEAFLVLSGECVLVVEEEERPLR
                     RGDFVHLPEGTAHVLVGAGDGPSSVLMVGTRKDPEELLYPVSETAAKYAASAERETND
                     EAEAYGPHNVLRPTNLGLPW"
     gene            complement(3780..3998)
                     /locus_tag="VF517_03620"
     CDS             complement(3780..3998)
                     /locus_tag="VF517_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052055.1"
                     /translation="MADLTNRPKYTPRRTREQRAFRLVQVGGVAGAVAVVGFVLALFG
                     VIGLGIPLLAAIIAVVSIVLFRSTVGKR"
     gene            4043..>5338
                     /locus_tag="VF517_03625"
     CDS             4043..>5338
                     /locus_tag="VF517_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052056.1"
                     /translation="MELGALQQQPAGAAAAPRLRPLDVGQKLDAAIKLATRNLGTLTA
                     IVLIVLVPINLLSFLITVSTLPDDFTAGGQLGPGATPDAGADSSGTFVAGQLVVNLLA
                     FVAFFLVPAVCFQAIGQAYLGERTGWRESLVYGLRRLHSILWIVLLMGLALLLVFIAG
                     VFFIGVAFLFPLLLLVVIPLALAPFVYLGVSWSLALPALLLEGARGVKALGRSHSLVQ
                     GRWWRTFGLLVLAWLLVLFVTTVVAGALAGVTYVVSDENTVTAVGLNFLANLVASVLT
                     TPFLATVTIVLYFDLRVRKEAFDLQLLAGAIGGRAPSEALARKELPWEAPPHGAPPPP
                     GWAPPPHGAPPPQAWAPPPGSAPPHGAPPAGWGHAPSLAPPLPPQGGWPPQLQAPQGW
                     GGPPQGGPAPTHAPPLPQAPPPHAPPPPPTPWAPPARQWD"
BASE COUNT          671 a         1855 c         1985 g          827 t
ORIGIN      
        1 cgctcgggcc gtccttcggg atcgcgcccg cggggacgtg gacgtggaac tcgcggtcct
       61 cgaaggcgtc ctcgtcgacg tccaggcgct cggcgtggcc gcgcacgtag gacagggcga
      121 tgcgcgccga ctccttcatc acgtcgccga gctgtcctgt gagcacgaga tcgttcttgc
      181 ccttcatgcc ggtggcctcg acgaacagca catcgccgcc ggtcccggtg accgcgagcc
      241 cggtggccac accggggatc gccgtgcgct cggcggcctc ctggaagaag cgctggcgcc
      301 cgagggcgtc gcgcacggcg tcggcctcga tcgcgaccgg tgcctcggcc gatcccgatg
      361 cgatccgcgt cgccgtcttg cggagcacgg tgcccagctc gcgctcgagc tggcgcacgc
      421 cggcctcgcg cgtgtactcg gtgacgaccg tgcggagcac gtcgtccgac accgtcacct
      481 cgtcctcgcg caggccgttg cgctcgcgct ggcgcggcca caggtagccg cgggcgatcg
      541 ccagcttctc ggcggtggtg tagccgtcga agcggatgat ctccatgcgg tcgagcagcg
      601 ggccggggat cgtctcggcg acgttcgccg tggcgatgaa cacgacctcg gagaggtcga
      661 gctcgacgtc gaggtagtgg tcgcggaacg agtggttctg cgctgggtcg aggacctcga
      721 gcagggccgc cgacggatcg ccgcgccagt cggcgccgac cttgtcgacc tcgtcgagca
      781 tgatcaccgg gttcatcgtc ctggcgtcgc gcagggcacg caccaggcgt cccggcagcg
      841 cgccgatgta ggtgcgccgg tgcccgcgga tctcggcctc gtcgcgcacg ccgccgagcg
      901 acatgcgcac gaactcgcgg ccggtggcac gcgcgatcga ctcgccgatc gaggtcttgc
      961 cggtgccggg cgggccgatc agggtgagga tcgccccgct cttgcggtcc tccgcgatgc
     1021 cgcgctcctt gcgcagcctg cgcacggcga tgtactcggt gatgcggtcc ttgacctcgt
     1081 cgagcccggc gtggtcggcg tccaggacct tgcgggcatg caccgggtcg agccgctcgt
     1141 ccgagcgttc cgaccacggc accgcgatca gccagtcgag gtagctccgg atcatcgacg
     1201 actcgccgct ctgctcaccg ctgcgctcga gccgggccag ctccttctcg gcctgctcgc
     1261 gcacctcgtc gggcatgccg gcctcttcga tctgggtgcg gtagtcctcg accaccgagc
     1321 cgtcgtcgtc gcccagctcc ttgcggatcg actccatctg cttgcgcagg aagtactcac
     1381 gctgctggtt ctcggtgccg gagcgaacgt cctcgcggat gcgcgcgcgc acctcgagct
     1441 cggtcaggcg gtcacgctgg aacgcgaggg acttctcgag gcgctcggtc acgtcgaggg
     1501 tctcgagcag ctcgatcttc tgctcgtagg agatgtcggg ggagtagccg gcggtgtcgg
     1561 ccagcgcgcc cggctcgctg atcgagcgca ggaacgcctg gatgcggccg tcgtcgccgc
     1621 gcagctcgag gatctcctcg accacggcgc ggtactcgcg cgcgagctcg cgggtgcgcc
     1681 cgtcgacggg ctcgtcgtcg gggtggggct cgacctccac gcggaggtcg ccgctcgggc
     1741 cggtctgcgc ggcgccgatg cgcccgcggt gcagcccgac caggctcacg gcgcggccgc
     1801 cgccgggcag ccgcacgcgc tcgaccacgt cggccacggt gcctacgctt gcaaacgcgt
     1861 cctcgtgacg cgggaccagc accacgcgct cctcgtcccc gacctcgagg gcgagcgtca
     1921 agttcattgt ggggaagacg acggtgtcgt ccacgggtac gagcagaagc tggtccacgg
     1981 cgctttcctt tcggaagctg gttacgatac gtaagtgagt gtagtgggta gcctcgcggg
     2041 catgaagctc gagggcatcc accacatcac ctgcatcacc ggggacgcac cgcggaacgt
     2101 cgacttctac gcgcgggtca tgggcctgcg gctcgtgaag aagacggtca accaggacga
     2161 cccgaccatc taccacctct tctacggcga cgaggagggt gcgccggggc acgacctgac
     2221 gttcttcgag tacccgggcg cgcagccggg caaggcgggg gcggggatgg tgcaccggct
     2281 gatccatcgg gtcgggtccg ccgacacgct cgacttctgg gccgagcggc tcggggcgga
     2341 ggaggtcgcg acacggcgcg agaacgggtc gctcgtgttc gccgacccgg aggggctcga
     2401 gcacgagctg gcggtcttcg aggtgccgga cccgccgctg atcgcccatg cgagcgaggt
     2461 accggacgag cacgcgctgc aagggttcga ggcggtgcgc acgtacaccg cggaccccga
     2521 ggcgagccgg cggttgctcg aggaggggct cgggttcgag cagcgctcag gcgacgagtg
     2581 ggaggcgcgc ggcggcgagc gcggcggacg gatggtgtac gaccgcagcc aggtgcgcgg
     2641 cgtgcagggc gccggcacgg tgcaccacgt ggcgtggtcg gtcgggctcg acgagctcga
     2701 cgcgtggcgc gagcgcgtgc tggccgccgg cgcgcggccg acgcccctca tcgaccgctt
     2761 ctggttccac tcgatctact tccgcgagcc gagcggcgtg ctcttcgagc tggccacgcg
     2821 cggccccggc ttcaccgccg acgagccgct tgacacgctc ggggaggccc tgacgctgcc
     2881 gccggactac gagcagtacc gcgagcgcct cgagcaggtg ctcaccccgc tgcccgaccc
     2941 gcgccagtgg aggcgggccg ggtcgcccca gtcggggtga cgaacgaggc caagctcctc
     3001 gtattcgtcc ggtacgtgct gccggcgctg attgtcgtgg ccgggcttgc gacgatcctg
     3061 atcggcgact cgaagagcgc gctgcacggc ggcctcggga tcgtcggcgg ggggatcgcc
     3121 gtgctgctgc tcagctactt ctaccgcgtg ggcgccagcg gcgagctcga gcgcgacgcc
     3181 gaggtggagg ctcgcgacta cttcgaccgc cacggccgct ggccggacga ggaccagcag
     3241 ctaccaggga agtcctaggt tcgtgggccg cagcacgttg tgcgggccgt atgcctcggc
     3301 ctcgtcgttc gtctcgcgct cggctgaggc cgcgtacttg gcggccgtct cgctgacggg
     3361 gtagaggagc tcctccgggt ccttgcgggt gccgaccatc agaaccgagc tcgggccgtc
     3421 cccggcaccc acgagcacgt gtgcggtgcc ctccgggagg tggacgaagt cgcccctgcg
     3481 cagcggtcgc tcctcctcct cgacgaccag cacgcactcg ccgctcagga ccaggaatgc
     3541 ctcctgggcg ttctcgcggt gatagagcgc cgacggctgg ccgggctcga gcacgcggac
     3601 gttgatgccg aactcgggga acgaggcctg gacgcggtcc tcgaagcgtg ccgagctgcc
     3661 gaagcggtca gagctgatgg ccttcgcgtc ggcgacgttg acgacgaacc agccctcgct
     3721 cgccgggatc acgccatctt ccgtggtgcg gagctcggcc tctggcaccg gctcgaggct
     3781 caccgcttgc cgacggtgct gcggaacagc acgatgctga ccacggcgat gatcgcggcg
     3841 agcagcggga tcccgaggcc gatgacgccg aacagcgcga ggacgaatcc cacgaccgcc
     3901 acggcgccgg cgacgccgcc gacctggacg agcctgaacg cgcgctgctc gcgcgtgcgg
     3961 cgcggcgtgt acttcgggcg gttggtcagg tcggccatgc gtcgctagag atcctaccgc
     4021 cggggtagag tccgccgggt cgatggagct gggggccttg cagcagcagc ccgccggagc
     4081 ggcggcggcg ccccgcctgc gtccactcga cgtggggcag aagctcgacg cggcgatcaa
     4141 gctcgcgacg cggaacctcg ggacgctcac ggcgatcgtc ctgatcgtcc tggtgccgat
     4201 caacctgctg tcgttcctga tcacggtctc gacgctgccg gacgacttca cggccggcgg
     4261 tcagctcggg ccgggcgcga cgcccgacgc cggcgccgac tcctccggca ccttcgtcgc
     4321 cggccagctc gtggtgaacc tgctcgcgtt cgtcgcgttc ttcctggtgc cggcggtctg
     4381 cttccaggcg atcgggcagg catatctcgg tgagcggacg ggctggcgcg agtcgctcgt
     4441 ctacgggttg cggcggctgc actcgatcct ctggatcgtg ctcctgatgg ggctggcgct
     4501 gctgctggtc ttcatcgcgg gggtcttctt catcggggtc gcgtttctgt tcccgctgct
     4561 gctcctggtg gtgatcccgc tggcgctggc gccgttcgtg tacctcgggg tgtcgtggtc
     4621 gctggcgctg ccggcgctgt tgctcgaggg cgcgcggggg gtcaaggcgc tcgggcggtc
     4681 gcattcgctc gtgcaggggc gctggtggcg gacgttcggg ctgctcgtgc tggcgtggct
     4741 gctggtcctg ttcgtgacga cggtggtggc gggcgcgctc gcgggggtga cgtacgtcgt
     4801 gagcgacgag aacacggtga cggcggtcgg cctgaacttc ctggcgaacc tcgtggcgag
     4861 cgtgctgacg accccgttcc tggcgaccgt gacgatcgtt ttgtatttcg acctgcgggt
     4921 gaggaaggag gcgttcgatc tgcagctgct cgcgggggcg atcgggggcc gggcgccttc
     4981 ggaggcgctg gcgcggaagg agctgccgtg ggaggcgccg ccgcatgggg ctccgccgcc
     5041 gccggggtgg gccccgccgc cgcacggggc cccgccgccg caggcatggg cgccgccgcc
     5101 gggctcggct ccgccgcacg gggctccgcc ggcgggctgg gggcacgcgc cgtcgctggc
     5161 tccgccgctg ccgccgcaag gcgggtggcc gcctcagctt caggcgccgc agggctgggg
     5221 agggccgccg cagggtggac cggcgccaac ccacgcaccg ccgctgccgc aagcaccgcc
     5281 gccccacgca ccgccgcccc cgccgacgcc ctgggcaccg cccgcgcgac agtgggac
//
LOCUS       DASVPL010000079         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_166618, whole genome shotgun sequence.
ACCESSION   DASVPL010000079 DASVPL010000000
VERSION     DASVPL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166618"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF517_03630"
     CDS             complement(<1..357)
                     /locus_tag="VF517_03630"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052057.1"
                     /translation="MASELISVEEARSRVLAAVRPAAAETVPCERALGRVLAEDVASG
                     EDLPPFDSSAMDGYAVAPGASGELAVLGEARAGAPADRAVGEGEAIAISTGATVPEGA
                     GAVVPIERVERVGDRHI"
     gene            562..1521
                     /locus_tag="VF517_03635"
     CDS             562..1521
                     /locus_tag="VF517_03635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8052058.1"
                     /translation="MDSPLSRLDESRGEIAKAWLQRFVERATLEEIERLPMQRLVREL
                     PELITEIARAAAAPVPETEANYLDWAQRLVELSGRDSIAAQELVRDLAVLQSAMISTL
                     HRGLERLDPVAALETVERLAELFSAMQADAVGEVLTVRSGETEWLGETDGLTGLHNTR
                     FMQQHLHHLVGVQKRYGHPFALLLVDIDGLKRINDAYGSGAGDRTLMGVATALGETIR
                     TVDTPIRMGGDEFCVLLPHQTASRAKVLADRLAGAIERIETPAPHPLRVAIGVVSCPQ
                     HSTQADVLLEQADGAMYRAKAAGESVAVGDGAEQAPANGDIAE"
     gene            complement(1660..2277)
                     /locus_tag="VF517_03640"
     CDS             complement(1660..2277)
                     /locus_tag="VF517_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8052059.1"
                     /translation="MTPDVLRCLILDEHPVVRQGIRALLERELPGVEVADAPSAEVAA
                     RAGGPSPDVVVVDPSHAGGNVEAMVAELRESLDSAIVVFTGNGGPRLLADALKAGVKG
                     YVRKDSPPEDLVRAIRAAHAGEFYVDPALSSTIVLEEGERTLTARQREILQMLADGLQ
                     TDAVARELGLSTETVRTHTKRILAKLHADTRTQAVAIAIRNGLIQ"
     gene            complement(2421..2552)
                     /locus_tag="VF517_03645"
     CDS             complement(2421..2552)
                     /locus_tag="VF517_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052060.1"
                     /translation="MREERVEQRAGQDPLTSALRAGMAVGDAGVKVLAKIVQRLPRR"
     gene            2590..>2881
                     /locus_tag="VF517_03650"
     CDS             2590..>2881
                     /locus_tag="VF517_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052061.1"
                     /translation="MPRPPDFARAVLASTLLPPALIAFAAFPRDVLAFARDALDPVAF
                     ARVALDPVAFLRVPPEPAAFAEPPAFARDAPEPAAFAEPAVFARDALDPAAFA"
BASE COUNT          386 a         1096 c         1004 g          395 t
ORIGIN      
        1 aatgtgtctg tcgcccacgc gctcgacccg ttcgatcggc accaccgcgc ccgccccctc
       61 ggggaccgtc gccccagtgg agatcgcgat cgcctccccc tcgccgaccg cgcggtccgc
      121 gggtgcgccc gcgcgcgcct cgcccaagac cgccagctcg ccgctcgcgc ccggcgcgac
      181 cgcgtagccg tccatcgccg aggagtcgaa cggcggcagg tcctcgccgc tcgcgacatc
      241 ctccgccagc acccgaccca gcgcgcgctc gcacggcacc gtctcggccg cggcggggcg
      301 caccgccgcc agcacgcggc tacgcgcctc ctccaccgag atcagctcgc tcgccacgcg
      361 cgcatattca cacgcgcccg ccagaacccc gctctttgcg gatctttcgg gcctgggcac
      421 ccccggtttg gcagatatct tgcatggaga acgccgggca tttgcagtcc ggaccgaggg
      481 gtacagtcgg cgatcacctt gatcaccgac ccgccgacaa tcaccgccag ccgcctttcc
      541 gccgcaccca cgcacgcgcc tgtggactcg ccactgtccc gtctcgacga atcgcgcggc
      601 gagatcgcga aggcttggct tcagcgtttc gtcgagcgcg cgacgctgga ggagatcgag
      661 cggctcccga tgcagcggct cgtccgcgag ctgccagagc tgatcacgga gatcgcgagg
      721 gcggccgcgg cgcccgtgcc ggagaccgag gcgaactacc tcgactgggc gcagcgcctg
      781 gtcgagctga gcggccgcga cagcatcgcc gcgcaggagc tcgtccgcga tctcgcggtc
      841 ctccagtcgg cgatgatctc gacgctgcac cgcggtctcg aacggctcga cccggtggcg
      901 gcgctcgaga cagtggagcg gctcgctgag ctgttcagcg cgatgcaggc ggacgcggtc
      961 ggggaggtgc tgacggtccg ctcgggcgag accgagtggc tcggcgagac cgacgggctc
     1021 accgggctgc acaacacgcg cttcatgcag cagcacctgc accacctggt cggcgtccag
     1081 aagcgctacg ggcatccgtt cgcgctgctg ctcgtcgaca tcgacgggct caagcggatc
     1141 aacgacgcct acggcagcgg cgcgggcgac cgcacgctga tgggggtcgc gacggccctc
     1201 ggcgagacca tccgcaccgt ggacacgccg atccggatgg gcggcgacga gttctgcgtc
     1261 ctcctcccgc accagacggc gtcccgcgcg aaggtgctcg cggatcgtct cgccggggcg
     1321 atcgagcgga tcgagacacc cgcgccgcac ccgctgcggg tggcaatcgg cgtcgtctcg
     1381 tgcccgcaac actcgacgca ggccgacgtc ctgctcgagc aggccgacgg cgcgatgtac
     1441 cgcgcaaagg cggccggcga atccgtggcc gtgggcgacg gcgccgagca ggcgcccgcc
     1501 aacggcgaca tcgccgagta gcgcggcggc gatcctggcg cggtgcaaca cgcactccta
     1561 gcggggtgcg gggcggcggc tctccccgcg gggactggtc gatccaagca cccgcatacg
     1621 gtggtcaatg tcgaccagta ggggctcggg ccccggggtc tactggatga gaccgttgcg
     1681 gatcgcaatc gccacagcct gcgtccgcgt gtccgcgtgc agcttggcga ggatccgctt
     1741 ggtgtgagtg cgcacggtct cggtagagag cccgagctca cgcgcgaccg cgtcggtctg
     1801 aaggccgtcg gcgagcatct ggaggatctc gcgctggcgg gcggtgagcg tgcgctcgcc
     1861 ctcctcgagc acgatcgtcg acgacagcgc cgggtcgacg tagaactccc cggcgtgtgc
     1921 agcgcggatc gcacggacga gatcctcggg cggggagtcc ttgcgcacgt agcccttgac
     1981 gccggccttc agcgcgtcgg caagaagtcg cgggccgccg ttgccggtga agacgacgat
     2041 cgccgagtcg agcgactccc gcagctcggc caccatggcc tcgacgttgc cgcccgcgtg
     2101 cgatggatcc accaccacga cgtcggggga gggaccgccg gcgcgcgcgg cgacctcggc
     2161 gctcggcgcg tcggccacct cgacgccggg aagctcacgc tcgagcagcg cgcggatgcc
     2221 ctggcggacg acgggatgct cgtccaggat caggcaccga agcacgtccg gagtcatcgc
     2281 aacagcggtt ccgccggccc gcgtgcgcgg gccagcgtgc acgtaggggt cacggaatcc
     2341 accctaccgc tatttgggcc gcctgtcacc gtcctggttc caggcgctcc ccggtggcgg
     2401 gtaccgcggc cggggcgtgg tcagcggcgc ggcaggcgct gcacgatctt cgcgagcacc
     2461 ttgaccccgg cgtcgccgac cgccatgccg gcgcgcagtg ccgaggtcag cggatcctga
     2521 cccgcgcgct gctcgacgcg ctcctcgcgc accgccgtgc gcggagcccg ttcgccggcc
     2581 gaagccccct tgccacgccc gcccgacttc gcgcgcgcgg tgctcgcgtc cacgttgttg
     2641 ccgcccgcgc tgatcgcctt cgcagccttc ccgcgcgacg tgctcgcctt cgcgcgcgac
     2701 gcgctcgacc cggtcgcctt cgcgcgcgtg gcgctcgacc cggtggcctt cttgcgggtc
     2761 ccgcccgagc cggcggcctt cgcggagccc ccggccttcg cccgcgacgc gccggagccc
     2821 gcggccttcg cggagcccgc ggtctttgcc cgcgacgcgc ttgacccggc ggccttcgcg
     2881 c
//
LOCUS       DASVPL010000080         5002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_323030, whole genome shotgun sequence.
ACCESSION   DASVPL010000080 DASVPL010000000
VERSION     DASVPL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323030"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..286)
                     /locus_tag="VF517_03655"
     CDS             complement(35..286)
                     /locus_tag="VF517_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052062.1"
                     /translation="MWSIRLTDSQRDALRLLLDAQGELGPSLTGAREALDLARWDDLP
                     EAHLPWDRVSELAGLQGCGEADVVWDLATGMPAPRADAA"
     gene            complement(329..1693)
                     /locus_tag="VF517_03660"
     CDS             complement(329..1693)
                     /locus_tag="VF517_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX8052063.1"
                     /translation="MTALALIALFALVAINGFFVAAEFSLVRVRREKMEALVEERARG
                     AALSIRQLDAIDRYLSACQLGITMASIGIGFLGEPAIASLLEDPLGEVVGHGVAVALA
                     FAVAFTVATALHITTGEQVPKIFAISRPEAVARRIARPLQWFASSFRPFEWALTRISN
                     GILRMFGVNAQAEFEEQTRSEDLKMLIARGVTGGTLDPGEAGMLSGVFHLHEQEARQV
                     MTPIPAVVTVDLSENVQTALRRCVDSGHTRLVVTEDDNTDRVKGIVHNNSLARLLMAQ
                     GADAAIDTAVKDALIVPETKALDDLLADLQRERSSVAVVVDEYGRTVGLVTVEDIIEE
                     VVGEISDETDPLGGAVRRLANGDWYVRGHVPITDLEDYGMHLPVESDAYNSIGGLVFS
                     ELGRLPKRGDMVRANGYSLRVESVRENRVEAVRIRDHEPDRRGGPRGGESTPQPEESV
                     RDGG"
     gene            1801..3366
                     /locus_tag="VF517_03665"
     CDS             1801..3366
                     /locus_tag="VF517_03665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8052064.1"
                     /translation="MKTQLDRRTFLAGTGALTLGGVPVGARAANTRHTIPFAKDGAFA
                     EGVASGEPGTRTATLWTRLSGLSADRRVTLEIAHDPDFRRLALRRDMVARLSEGGAVK
                     VRVPARAKLKPGERYWYRFATKTTSSPVGRFQTMRPPDSREPVRIAFFSCQDWQAGYY
                     GAHRTIAEDDSLDFVLCLGDYIYEKNYYEGPREDRLGANGDGEVETREEYRQKYALYR
                     SDADLRAMHAAHPFVPIWDDHEVEDNWAGATGGHQTPNRRVPYEQRRFNGLRTFYESM
                     PLRRPERGGFKLYRSIPLGASAELFLLDERQYRDPQPCDDTFVIPCPEAETEPRRFLG
                     DAQKAWLKGALEGSRAAWKLVANQAMIMALENGPSSPSNKDQWDGYGVERRDIMEHVA
                     SRNIQDVAFLTGDIHTFFAGEVGTDGRGPESVATEFVGGSITSLGIPESVESVSNVPA
                     DVVFQATQNFRTVNPHLKFDDQKRRGYGVVEARADELRVEFRAVEALRRTTTAEPIAR
                     FRVARGTPRVEVL"
     gene            complement(3361..3768)
                     /locus_tag="VF517_03670"
     CDS             complement(3361..3768)
                     /locus_tag="VF517_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8052065.1"
                     /translation="MRDRTRTRLRGLVHDALVVIERDLERDIALDDVARTIATSRRSL
                     QRAFEGEGITFREAVAVARMRRAKALLSQGGMPVYRVADRVGYRSKAEFTKAFKRYSG
                     QTPTDYMRALRDGDVAIEARANGNGGSLNGHPS"
     gene            complement(3917..>5002)
                     /locus_tag="VF517_03675"
     CDS             complement(3917..>5002)
                     /locus_tag="VF517_03675"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX8052066.1"
                     /translation="VDRGAGRDQVLVARSADSGRTWSKPVRATGRPAGAVDEWWPAVA
                     LGPKGRATIAWVDRSTDRERVYFAQSADGGRRWGAPRPLDPSPPRGVAQWRPALAAGR
                     GEVVHAAFVDEREVSADDALPQAHVFYSRIRAGQAEPARRLDQGEPEALAARLDNSWA
                     PRVSARGERVLVTWIDFARYDWDVFARESGDGGGSFADQRDVNSTPPADEALDDSPEA
                     ALGGTKPFVAWTDWRKNDSSATTPLRQYDTFVAVPGAPNVQADPYGSGPVSTFAPSIC
                     ATRGDDALVAFQDASRGQNDIRLVHLRGGTRRGPARRVDDAGDRGGNAWRPRLGCWGD
                     RVVAAWEDERDGPAQVYAAVSTAGRLR"
BASE COUNT          700 a         1820 c         1804 g          678 t
ORIGIN      
        1 ggcgctttgt caagcgccga tgtgccagcc ggttctaggc ggcgtccgcg cgcggcgcgg
       61 gcatcccggt ggccaggtcc cacaccacgt cggcctcgcc gcagccctgg agccccgcga
      121 gctcgctcac gcggtcccac ggcaggtgcg cctcgggcag gtcgtcccag cgcgcgaggt
      181 cgagcgcctc gcgagcgccg gtgagggagg gaccgagctc tccctgcgcg tctaatagga
      241 gtcgcagcgc gtcacgctgt gaatcggtga gtcgtatcga ccacatctgg caggacctcc
      301 tgggaggaga agctcgtgcc gctcgaaact agcccccgtc gcggacggat tcctccggct
      361 ggggggtcga ctcgcctccg cgcggcccgc cgcggcgatc cggctcgtgg tcgcggatgc
      421 gcactgcctc cacgcggttc tcgcgcacgg actcgacgcg gagggagtag ccgttcgcgc
      481 ggaccatgtc cccgcgcttc ggcagccgcc cgagctcgct gaacaccagg ccgccgatcg
      541 agttgtaggc gtcggactcc accggcaggt gcatcccgta gtcctcgagg tcggtgatcg
      601 gcacgtgacc gcgcacgtac cagtcgccgt tggcgagccg gcgcacggcg ccgccgagcg
      661 ggtcggtctc gtcggagatc tcccccacga cctcctcgat gatgtcctcg accgtgacga
      721 gcccgaccgt ccggccgtac tcgtccacca ccaccgcgac ggacgagcgc tcgcgctgca
      781 ggtcggccag caggtcgtcg agcgccttgg tctccggcac gatcagggcg tccttcaccg
      841 cggtgtcgat cgcggcgtcc gccccctgcg ccatcagcag ccgggcgagc gagttgttgt
      901 gcacgatccc cttgacgcgg tcggtgttgt cgtcctcggt caccacgagc cgtgtgtggc
      961 ccgagtccac acagcggcgc agcgcggtct gcacgttctc tgagaggtcc accgtgacga
     1021 ccgccgggat cggcgtcatc acctggcgcg cctcctgctc gtgcaggtgg aagacgcccg
     1081 agagcatccc cgcctcgccg gggtcgagcg tgccgcccgt gacgccgcgg gcgatcaaca
     1141 tcttcaggtc ctccgagcgc gtctgctcct cgaactccgc ctgggcgttg accccgaaca
     1201 tccggaggat cccgttcgag atgcgcgtga gcgcccactc gaacggccgg aagctcgagg
     1261 cgaaccactg gagcgggcgc gcgattcgcc gcgccacggc ctccgggcgc gagatcgcga
     1321 agatcttcgg cacctgctcg ccggtggtga tgtggagcgc ggtggcgacc gtgaacgcca
     1381 ccgcgaacgc cagcgccacc gccacgccgt ggccgacgac ctcgccgagt gggtcctcga
     1441 gcagcgacgc gatcgccggc tcgccgagga agccgatgcc gatcgaggcc atggtgatgc
     1501 cgagctggca ggccgacagg tagcggtcga tcgcgtcgag ctggcggatc gagagcgccg
     1561 cgccgcgggc gcgctcctcg accagcgcct ccatcttctc gcggcgcacg cgcacgagcg
     1621 agaactccgc ggccacgaag aacccgttga tcgccacgag ggcgaacagt gcgatgaggg
     1681 cgagcgccgt cacgacccgg tcagggcgat ccccggcggc cggtgcgtcg tgctacttcg
     1741 aggaggatcg tcgtcgctca tcggcggacg tgcgcaaggt agcagggata ctggaggcca
     1801 atgaagaccc aactcgacag acgtacgttc ctggccggca ccggtgcgct gacactcggc
     1861 ggggtgcccg tgggcgcccg ggcggcaaac acgcgccaca ccatcccgtt cgccaaggac
     1921 ggggcattcg cggagggagt ggcctcgggc gagcccggca cgcgcacggc cacgctctgg
     1981 acgcggctca gcggcctcag cgcggaccgg cgggtgacgc tcgagatcgc ccacgacccg
     2041 gacttccgcc ggttggcgct gcgccgcgac atggtcgcgc ggctctccga gggtggcgcc
     2101 gtgaaggtgc gcgtgcccgc gcgcgcgaag ctgaagccgg gcgagcgcta ctggtaccgc
     2161 ttcgcgacga agaccacgtc gagcccggtc gggcgcttcc agacgatgcg cccgcccgac
     2221 tcgcgcgagc cggtccggat cgccttcttc tcgtgccagg actggcaggc cggctactac
     2281 ggcgcgcacc gcacgatcgc cgaggacgac tcgctcgact tcgtcctctg cctcggcgac
     2341 tacatctacg agaagaacta ctacgagggc ccgcgcgagg accggctcgg cgcgaacggc
     2401 gacggcgagg tcgagacccg cgaggagtac cgccagaagt acgcgctcta ccgctccgac
     2461 gccgacctgc gcgccatgca cgccgcgcac ccgttcgtcc cgatctggga cgaccacgag
     2521 gtcgaggaca actgggcggg cgcgaccggc gggcaccaga ccccgaaccg ccgggtgccc
     2581 tacgagcagc gccgcttcaa tggcctgcgc acgttctacg agtcgatgcc gctgcggcgg
     2641 ccggagcgcg gcggcttcaa gctctacagg tcgatcccgc tcggggcgag cgccgagctg
     2701 ttcctgctcg acgagcgcca gtaccgcgac ccgcagccgt gcgacgacac gttcgtgatt
     2761 ccctgccccg aagccgagac cgagccgcgc cgcttcctcg gcgacgcgca gaaggcgtgg
     2821 ctcaagggcg ccctcgaggg gtcgcgagcg gcctggaagc tcgtcgccaa ccaggcgatg
     2881 atcatggccc tcgagaacgg cccgagcagc ccgtcgaaca aggaccagtg ggacggctac
     2941 ggggtcgagc ggcgcgacat catggagcac gtcgcgagcc gcaacatcca ggacgtcgcg
     3001 ttcctgaccg gcgacatcca cacgttcttc gcgggcgagg tgggcaccga cggccgcggg
     3061 ccggagagcg tcgccaccga gttcgtcggc gggtcgatca cctcgctcgg catcccggag
     3121 tcggtcgagt cggtctcgaa cgtgccggcc gacgtggtgt tccaggccac gcagaacttc
     3181 aggaccgtga acccgcacct gaagttcgac gaccagaaac gccgcggcta cggcgtggtg
     3241 gaggcgcggg cggacgagct gcgggtggag ttccgcgcgg tcgaggcgct gcggcgcacc
     3301 acgaccgccg agccgatcgc gcgctttcgc gtggcgcgcg gcaccccgcg ggtcgaggtc
     3361 ctatgagggg tggccgttga gactgccgcc gttgccgttg gcacgggcct cgatcgccac
     3421 gtcgccgtcg cgcagggcgc gcatgtagtc cgtcggggtc tggcccgagt agcgcttgaa
     3481 cgccttggtg aactcggcct tcgagcggta gccgacgcgg tcggccactc ggtagaccgg
     3541 catcccgccc tgcgacagca gcgccttcgc gcggcgcatg cgcgccacgg ccacggcctc
     3601 gcggaacgtg atcccctcgc cctcgaacgc gcgctggagc gagcgccgcg acgtggcgat
     3661 ggtcctcgcg acgtcatcga gtgcgatgtc gcgctcgaga tcgcgctcga tcacgacgag
     3721 cgcgtcatgg acgagtccgc gcagccgggt ccttgttctg tccctcacct tcgtccctcc
     3781 gcggcattgt gggcgccgca gcccttgttt gcgagtccta gcagtccgta tgacaacgcg
     3841 ccaaaggatg acggtgttgg gcggagtccc cgggattggc acgtagccgc tacgggcggt
     3901 agcggaggcg ataccgctag cgcaggcgtc cggcggtgct gaccgccgcg tagacctgcg
     3961 cgggcccgtc gcgctcgtcc tcccaggcgg ccaccacgcg atccccccag cagcccagtc
     4021 gcggccgcca agcgttgccg ccgcggtcgc cggcgtcgtc gacccgccgc gccgggccgc
     4081 gccgggtgcc gccgcgcagg tgcaccagcc ggatgtcgtt ctggccgcgg ctcgcgtcct
     4141 ggaacgccac gagcgcgtcg tcgccgcggg tggcgcagat cgatggggcg aacgtggaga
     4201 ccgggccgct gccgtagggg tcggcctgca cgttgggggc gccggggacg gcgacgaagg
     4261 tgtcgtactg gcgcagcggg gtggtggccg agctgtcgtt cttgcgccag tcggtccagg
     4321 cgacgaacgg cttggtcccg ccgagtgccg cctcgggcga gtcgtcgagc gcctcgtcgg
     4381 ccgggggcgt gctgttcacg tcgcgctggt cggcgaacga cccgccgccg tcgcccgact
     4441 cgcgcgcgaa cacgtcccag tcgtagcggg cgaagtcgat ccaggtgacg agcacgcgct
     4501 cgccgcgcgc agagacccgc ggcgcccacg agttgtcgag ccgcgccgcc agcgcttccg
     4561 gttcgccctg gtcgagccgc cgcgcgggct cggcctgccc cgcgcggatg cgcgagtaga
     4621 agacgtgggc ctgcgggagc gcgtcgtcgg cggagacctc gcgctcgtcc acgaacgcgg
     4681 cgtgcaccac ctcgccgcgg ccggccgcca gcgcggggcg ccactgggcc acgccgcgcg
     4741 gaggcgacgg gtcgagcggc ctcggcgcgc cccagcgccg gccgccgtcg gccgactgcg
     4801 cgaagtagac gcgctcgcgg tcggtcgagc ggtcgaccca ggcgatcgtg gcgcgcccct
     4861 tcggaccgag cgcgaccgcc ggccaccact cgtccaccgc ccccgccggg cggccggtcg
     4921 cgcgcacggg cttcgaccac gtgcggccgg agtcggcgga gcgggccacg agcacctggt
     4981 cgcgcccggc gccgcggtcc ac
//
LOCUS       DASVPL010000081         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_323081, whole genome shotgun sequence.
ACCESSION   DASVPL010000081 DASVPL010000000
VERSION     DASVPL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323081"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VF517_03680"
     CDS             <1..289
                     /locus_tag="VF517_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052067.1"
                     /translation="DLRAAGAEGSREARLAEGLLADAETRVIYAQPTDELERTRELLG
                     LTETEVELLPTLRRGEALWKVGQRSFLVYHRVSTFEARLVDTDARMAVHPR"
     gene            286..2031
                     /locus_tag="VF517_03685"
     CDS             286..2031
                     /locus_tag="VF517_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEX8052068.1"
                     /translation="MNAPAPGHATGLAPWEIAAALLLASTFLLGALLWLWGGIAGALF
                     GGGWPEASAADTARVAVRLPSRLGDPAEAWPSPVRDELPGAPGFYLSLALLVAALAVV
                     GYLLIRAFRRGRAGGAARTRRPRTAAWARRGELRPLTVRAPVAGRLTLGRIGGRLAAA
                     EPRQSVIVVAPTQSLKTTGLAIPAVLEWEGPVVAASVKSDLLRDTLACRRGVGQVLVY
                     DPTGATGVPSSGWTPLAGSATWGGAQRTAAWLTSAAQPARAALADADFWYAAAAKLLA
                     PLLFAAVSSGRPMADVVRWVDTQEEAEVQQALEDAGEKAALVAAEASWRRDERQRSSI
                     YTTAETALAAYADPGVMASADTSEITPEALLDGGSHTLYVCAPAHEQQRLRPLFATLT
                     QTIVSAVFERASRTGKPLDPPLLLVLDEAANIAPLRDLDALAATAAGQGIQLVSVFQD
                     LAQLHERWGARSGSIVNNHRARIFGGGIADTETLDYVGRLLGDAEVRQHSSTAGADGR
                     GSTTESSGFRALAPANVLREGRPGRAVLVYGHLPPAELELRPWFADRSLRRLVAGEHP
                     VRAREDLAEGDDAAA"
     gene            2135..2497
                     /locus_tag="VF517_03690"
     CDS             2135..2497
                     /locus_tag="VF517_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052069.1"
                     /translation="MHGPPQGAHAAPDAQIARARHVADRFLAHYVPFLYGRGRAERIP
                     NVSATVRRGLTRARARVSPASRMRRPRIAGLTLTPQARGTVIARAAIEDGGVASYPVT
                     FTLVLRGGRWIVNALGSD"
     gene            2504..3589
                     /locus_tag="VF517_03695"
     CDS             2504..3589
                     /locus_tag="VF517_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052070.1"
                     /translation="MAAATGSRFGQVAALGVASLIAAAVIGIAILGSLLGKDGCDAGS
                     PPSKAAERDIPRDFLALYQRLGARYGLPWPILAGIGKEECDHGRLRHPACTPRPGQSG
                     RGPANFAGAAGPMQIGISGAAGCMFCSVRVDGGGDGRIGTHDPADSIAMAARTLLEHK
                     GARKNRPLTDYRSAVRAYNGAGPQAEAYATRVLADARAYASWDFATASSASGCDGPGL
                     TGTGVRGKVVVAAGANRPGVQLRRTALDYLAAMAGLYGKPIIVSTGTNHDRLTVNGNV
                     SDHWDGHAADLGMAINGGSDGSRVGDLLMTACLRVAGVPAARAAAQAARGGLYTLTRG
                     ALRIQCIWKTYAGGNHYTHVHVGVQPG"
BASE COUNT          466 a         1364 c         1391 g          512 t
ORIGIN      
        1 cgacctgcgg gcagccgggg cggagggcag cagggaggcg cggctcgccg aaggcctcct
       61 ggccgacgcc gagacgcgcg tcatctacgc ccagccgacc gacgagctcg agcgaacgcg
      121 tgagctgctc ggccttacgg agaccgaggt cgagctgctg ccgacgctgc ggcgggggga
      181 ggcgctctgg aaggtcggcc agcggtcgtt cctcgtctac caccgggtct cgacgttcga
      241 ggcgaggctc gttgacaccg atgcgcggat ggcggtgcac ccgcgatgaa cgcgcccgcc
      301 ccgggtcacg ccacgggcct cgctccatgg gaaatcgcgg ctgcgttgct gctggcgtcg
      361 acctttctgc tcggtgcact cctctggctc tggggcggca tcgccggtgc actgttcggc
      421 ggtggctggc cggaggcgtc cgctgccgac acggcgcgcg tcgccgtgcg gctcccgtcc
      481 cgcctcggcg acccggcgga ggcgtggccg tcgccggttc gggatgagct gccgggggcg
      541 cccggcttct acctctcgct tgcactgctg gtcgcggcgc tggccgtggt gggctacctg
      601 ctcattcggg cgtttcgccg ggggcgcgcc gggggtgcgg cgcgtacgag gcgaccgcgt
      661 acggcggcct gggcccggcg gggcgagttg cgcccgctca cagtgcgcgc gccggttgca
      721 gggcgtctga cgctcggccg gatcggcgga cgcctcgccg cagccgagcc ccgccagtcg
      781 gtaatcgtgg tggcaccgac gcagagcctg aagacgacgg gcctcgccat tccggccgtg
      841 ctcgagtggg agggacccgt cgtcgcggcc agcgtcaaaa gcgacctcct gcgcgacacg
      901 ctcgcctgtc ggcgtggggt cgggcaggtg ctcgtctacg acccgaccgg cgccaccggc
      961 gttccgagct ccgggtggac cccgctcgcc gggtccgcca cgtggggagg agcgcaacgc
     1021 acggccgcgt ggctgacatc ggccgcgcag ccggcccgtg ccgcactggc cgacgcggac
     1081 ttctggtacg ccgcggcggc gaagctgctc gcgccgttgc tgttcgccgc cgtttcgagc
     1141 ggtcggccga tggccgacgt cgtccgctgg gtcgacacgc aggaggaggc cgaggtccag
     1201 caggcgctcg aggacgccgg ggagaaggcg gcgctcgtcg ccgccgaggc gagctggcga
     1261 cgcgacgagc gccagcggtc gtccatctac acgaccgcgg agaccgcgct cgccgcctac
     1321 gccgaccccg gcgtgatggc ctcggccgac acctccgaga tcacgccgga ggctttgctc
     1381 gacggcggct cacacacgct ctacgtctgc gcgcccgccc acgagcagca gcgcctgcgc
     1441 ccgctgttcg cgaccctcac gcagacgatc gtgtcggccg tgttcgagcg ggcctcacgg
     1501 acgggcaagc cgctcgaccc tccgctgctc ctggtgctcg acgaggcggc caacatcgcg
     1561 ccgctccgcg atctcgacgc actagcggcc acggctgccg gccagggcat ccagctcgtc
     1621 tccgtcttcc aggacctcgc gcaattgcac gagcgctggg gcgcgcggtc tggatcgatc
     1681 gtcaacaacc accgcgctcg catcttcggc gggggcatcg ccgacaccga gacgctcgac
     1741 tacgtagggc gcctcctcgg cgacgcggag gtgcgccagc actcgtcgac ggcgggcgcg
     1801 gacgggcgcg gctcgaccac tgagtcgagc ggcttccgcg cgctcgcacc ggcgaacgtc
     1861 ctgcgcgagg gccgcccggg acgggccgtg ctggtctacg gacacctgcc gccggcggaa
     1921 ctcgagttgc gcccctggtt cgcggacagg tcgctccggc gcctggtggc tggtgagcat
     1981 cccgtgcggg cgcgcgagga cctcgcggag ggggacgatg cggccgcgtg aggcggcggt
     2041 cgctctcggc ctggtcgcgc tactccttct cgcgctgagc ggcggtgggt cccgtgaacg
     2101 cggcatttcc gcgcctcctc cgtccccgcc cgtggtgcac ggaccgcctc agggcgccca
     2161 tgccgcgccc gatgctcaga tcgcccgggc gcggcacgtc gccgaccgct tcctcgcgca
     2221 ctacgtgccg ttcctctatg gccgcggccg ggccgagcgg atcccgaacg tgagcgcgac
     2281 cgtccgaagg ggcctcacgc gcgcgcgtgc gcgcgtgagc ccagcgtcgc ggatgcggcg
     2341 gccacgcatc gcagggctga ccctgacgcc gcaggcccgc ggcacggtga tcgcacgcgc
     2401 cgcgatcgag gacgggggtg tggcctcgta tcccgtgact ttcacgctcg tcctccgcgg
     2461 cggtcgctgg atcgtcaacg cgctgggaag cgactgagcc gagatggccg cggcgacggg
     2521 cagccggttc gggcaggtcg cggccctcgg cgtcgccagt ctcatcgcgg cagcagtcat
     2581 cgggatcgcg atcctcgggt cgctgcttgg caaggacggc tgcgatgcgg gcagcccgcc
     2641 gtcgaaggcg gcggagcgag acatcccgcg cgacttcctc gctctttacc agcgcctcgg
     2701 cgcgcgctac gggctcccgt ggccgatcct ggccggaatc gggaaggagg agtgcgacca
     2761 cggccgcctc cgccacccgg cttgcacgcc gcggcctggc cagtcaggac gcgggccggc
     2821 gaacttcgcg ggcgcggcgg ggccaatgca gatcgggatc agcggggcgg ccggctgcat
     2881 gttctgcagc gtgcgggtcg acggcggtgg cgacggccgg atcgggaccc acgaccccgc
     2941 cgactcgatc gcgatggcgg cgcgcaccct gctcgagcac aagggcgcgc ggaagaaccg
     3001 gccgctcacc gactaccgca gcgcggtgcg cgcgtacaac ggcgcgggcc cgcaggccga
     3061 ggcgtatgcg acgcgcgtgc tcgccgacgc ccgcgcctac gccagctggg actttgcgac
     3121 ggcctcgagc gccagcggct gcgacgggcc tggcctgacg ggaacggggg tccgcggcaa
     3181 ggtcgtcgtc gccgcgggcg cgaatcgccc cggcgttcag ttgcgccgaa ccgccctcga
     3241 ctacctcgcc gcgatggcgg gcctctacgg caagccgatc atcgtcagca ccggcacgaa
     3301 ccacgaccgg ctgacggtca acggcaacgt ctccgatcac tgggacggcc acgccgcgga
     3361 tctcggtatg gccatcaacg gaggcagcga cggcagtcgc gtcggggacc tactgatgac
     3421 ggcgtgcctc cgggtcgcag gtgtcccggc cgcgcgcgcc gcggctcagg cggcgagggg
     3481 cgggctctac acgctcaccc gtggtgcgct gcggatccag tgcatctgga agacctacgc
     3541 cggcggcaac cactacacgc acgtccacgt cggagtccag ccgggatgag ccggtcagct
     3601 cctgtccgcg gtcactcgcg cgtggtcgag atcgggcttc ctggcgggcg cattgtcggc
     3661 gacggctcgg catccgcgac aggcctgcgg cctgcggcct tgccccagcg cctgcggcgc
     3721 tgcccttcgg gtg
//
LOCUS       DASVPL010000082         8458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_205130, whole genome shotgun sequence.
ACCESSION   DASVPL010000082 DASVPL010000000
VERSION     DASVPL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205130"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1500)
                     /locus_tag="VF517_03700"
     CDS             complement(133..1500)
                     /locus_tag="VF517_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8052071.1"
                     /translation="MRLEITSGADAGRTANVSGDLFTLGREGNTNLVLRDAKASRRHA
                     TLKALPDGRVELTDLGSRNGTFVNGQQVTGSVVLNGGEEIRIGDTVMRAVREQPAAPA
                     PAAAPPPPAAPPPPPGAAGPPQPQPAQPPPPLAPGFQPPTEPIPHAPQPVAQAPGARP
                     VVRQVDAPPPPGPSASTMQRLVIQRGLKRVTILGIVVLVLLVGVVVALVTGVFTGGGD
                     DELSPAEVVDQVRDRTLLVVNNKGFGSGGRGSGWVWDASEGLIVTNAHVTAGGQSYTV
                     STGEKLSISVGTDIKIQGEDAREAEVKGQALCDDIAVLQVKDTKGLETMPRVPSKKEL
                     KVGEEVVGVGYPSTINLTEGQERQDADLTGNTGVISTVSTTFPAIPGEGPDDPTVGPY
                     KDVILTDTIINQGNSGGPLVNREAQLVGMNSAQRTDVQGQFYAVGIDRINEVVPKLIE
                     GDDPC"
     gene            complement(1513..1884)
                     /locus_tag="VF517_03705"
     CDS             complement(1513..1884)
                     /locus_tag="VF517_03705"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8052072.1"
                     /translation="MITHHDGCFGCGQANLFGLQLETDGVRGRFFVKQDHQGPPGYAH
                     GGIVATALDEAMALALHAQGLDTRTRRLELDLLAPAPVGAFLEVEASVETREGGRIRT
                     RASAREGDTLVAEAVAVFVVS"
     gene            complement(1881..4442)
                     /gene="acnA"
                     /locus_tag="VF517_03710"
     CDS             complement(1881..4442)
                     /gene="acnA"
                     /locus_tag="VF517_03710"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX8052073.1"
                     /translation="MASLGDYRIFRLDELQAKYDVARLPYSLKVLLENALRNGHDESV
                     EAIATWDAKAEPSKEIAYEPARVLMQDFTGVPAIVDLAAMRDAADDPSKINPLVPAEL
                     VIDHSIQVDEFATKLAFVHNAEREFERNGERYAFLRWGQTAFDDFAVVPPNTGICHQV
                     NLEYLARVVYTRDGQAFPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQPISML
                     LPQVVGFRLTGELPEGSTATDLVLTVTQMLRAKGVVGKFVEFFGPGLPTLPLADRATI
                     GNMAPEYGATCGIFPVDDQTLRYLEFTGRDTELVEAYMREQGMFHDEGADDATYSDTL
                     ELDLGDVEPSLAGPKRPQDRVPLREAKASALEALGEMVEYKGQDEAVSESFPASDPPT
                     NGREDVSRESEVVAVADKTPQGEGLDHGAVVIAAITSCTNTSNPSVMLAAGLLAKKAV
                     EMGLESKPWVKTSLAPGSKVVTEYLERAGLLEPLSKLGFDLVGYGCTTCIGNSGPLPE
                     EISKEILDRDLAVCSVLSGNRNFEGRIHPEVRLNYLASPPLVVAYALAGRMDIDLYDD
                     FPLREIWPSQAEVTAAMQYVEREMFTRSYGEVFEGDENWNSIQVPEGDRYEWDDESTY
                     VKRPPYFEMEPYAGAVEGARVLAKLGDSVTTDHISPAGAIKKDSPAGRYLTERGVEDF
                     NSYGSRRGNHEVMMRGTFANVRLRNLLAPGTEGGVTIKDGRELPIFDAAMEYADEGVP
                     LCVIAGKEYGSGSSRDWAAKGPRLLGVRFVIAESYERIHRSNLVGMGILPLQAAVEVD
                     GDTVIGVPEIEDGQKTVTIDIDGSSVEATVRIDTPNEWQYYRAGGILPFVLNRLR"
     gene            complement(4435..5484)
                     /locus_tag="VF517_03715"
     CDS             complement(4435..5484)
                     /locus_tag="VF517_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052074.1"
                     /translation="MAVMETVARTVGEFGEAQSLWPRGDRPQAIRAAAEQFRARFKEQ
                     GQVLSVQTVDLVAAGYPLNFAFGGAAKGLNPFVSISNRLVVVRFEDFNGAEKVLAWEP
                     TVPEGSQEAPFYEQLVERYGEWLSNNVFATYYNDIGQALAKCGLTPEDVDYIAFDHLH
                     VQDPRMMLGTATQSAFYPNAKMIAQRAEVDTFRSIHPTQWAWYVEGGMDGVPEDKLVL
                     VEGDVEIGKGLAFLATPGHTDGNMSLCVNTPDGIWVSSENGVAADNWHPHLSKIPGVR
                     KWAEFFNRDVVMNSNTLEDSSDQYDSMIKEHAVADPDSRDPRWKQVFPSSELARFRRQ
                     WPVLPTHWFGGINYG"
     gene            5511..6647
                     /locus_tag="VF517_03720"
     CDS             5511..6647
                     /locus_tag="VF517_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HEX8052075.1"
                     /translation="MHEYERYENAFGDRDAPFAFVDLDAMWANAAEMLGRAGGKPIRV
                     ASKSVRCRELLARVLARDGFSGLMTYTLRESQWLDEHGFEDLLLAYPTADREALDREP
                     LPVLMVDSTEQLDLIGDRHARVCIEVDLSFPIAGRRIGPKRSPIRTPAQAVALARTAL
                     ARGFTLVGVMGYEGHLAGVGDRPEGRLRGMAIRAMQRASVREMAERRAEIVAAVREVA
                     PLEFVNGGGTGSLHTTSREDAVTELTAGSGFYAPALFDSYSAFTLRPAAMFALPVVRR
                     PSARVATCLGGGYIASGAAAGDRLPVPHLPAGLELDAQEGAGEVQTPVSGAAARHMRV
                     GDRVYFRHAKAGELCEHFDALLLVEGGRVVDEVPTYRGEGKLFL"
     gene            6708..6938
                     /locus_tag="VF517_03725"
     CDS             6708..6938
                     /locus_tag="VF517_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052076.1"
                     /translation="MSESDLREFIREQSLIANRRTDAFVKEISRQREEMRLHFEKQDR
                     KIDEMVAEGKAGRAALFAILDRLDNGGPATAG"
     gene            complement(6955..8253)
                     /locus_tag="VF517_03730"
     CDS             complement(6955..8253)
                     /locus_tag="VF517_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX8052077.1"
                     /translation="MPAPWRNWTGDQRCAPELVEEPASDEQVAAAIGRAARAGRPVRV
                     AGAGHSFSDIVLTDGHLLRLHRMNRVLAADASTGLAEIEAGASIRELGPALAEHGLAL
                     ENQGDVDPQSLAGAIATATHGTGARFPNLSAQVAGLRLIGDGGEAVDCSEGHEPELFR
                     AARVSLGALGVITRVTLRCAPLYTLRRLDAPRPLDETLERLDELVDGHERFELFVFPY
                     TRVALTRVTEATTDPPAPLAPAAEWVQDVLLENLALELFSRAGRALPRGIPAMNRAIA
                     RMLQPSERVDRSYRIYANQRRVRFTEMEYAVPREHAGEALERVLAMIERRRLPISFPI
                     EFRFVAPDDAHLSPSNGRATAYVAVHVYRGTEHETYFRAVESIMREYGGRPHWGKRHY
                     RTAADLAPDYPDWERFAAARERLDPNRRFRNDYTDRVLGP"
BASE COUNT         1167 a         2952 c         3048 g         1291 t
ORIGIN      
        1 catgaacgcc atcgtgccgg tggcgtcctg cagcagggtc tgatcgatcc gcagcccgat
       61 cggcgacccg ggcttcagct ccccgtcgac caggtggtcg gcgagaatct gccgggtcag
      121 gctgtcaggc acctaacagg gatcgtcgcc ctcgatgagc ttcgggacga cctcgttgat
      181 ccggtcgatc cccaccgcgt agaactggcc ctgtacgtcg gtgcgctggg ccgagttcat
      241 cccgacgagc tgcgcctcgc ggttcacgag ggggccgccg gagttgccct ggttgatgat
      301 cgtgtcggtg aggatcacgt ccttgtacgg cccgacggtc gggtcgtcgg ggccctcgcc
      361 ggggatggcc gggaacgtgg tgctcacggt cgagatcacg ccggtgttgc cggtcagatc
      421 cgcgtcctgg cgctcctggc cctcggtcag gttgatcgtc gacggatagc ccacgccgac
      481 gacctcctcg cccaccttca gctccttctt gctcgggacg cgcggcatcg tctcgagccc
      541 cttcgtgtcc ttgacctgga ggaccgcgat gtcgtcgcag agcgcctggc ccttgacctc
      601 ggcctcgcgc gcgtcctccc cctggatctt gatgtcggtg cccaccgaga tcgagagctt
      661 ctcgccggtg ctcaccgtgt agctctggcc acccgccgtg acgtgggcgt tggtgacgat
      721 gaggccctcg ctggcgtccc agacccagcc cgacccgcgg ccgcccgagc cgaagccctt
      781 gttgttgacg acgagcaggg tgcggtcgcg cacctggtcg acgacctcgg cgggcgagag
      841 ctcgtcgtcc cctccgccgg tgaagacccc cgtgacgagc gcgaccacca cgccgaccag
      901 cagcaccagc acgacgatgc cgaggatcgt gacgcgcttg aggccacgct ggatgacgag
      961 tcgctgcatc gtcgaggcgc tgggcccggg cgggggcggc gcgtccacct ggcgcacgac
     1021 cggtcgcgct cccggggcct gagccacggg ctgcggggcg tgcgggatcg gctcggtcgg
     1081 cggctggaag ccgggtgcca ggggcggcgg aggctgcgcg ggctgaggct gcgggggacc
     1141 cgcggcaccc gggggcggcg gtggggccgc ggggggcggc ggagcggccg ccggcgcggg
     1201 cgccgccggc tgctcgcgca ccgcgcgcat caccgtgtcg ccgatgcgga tctcctcgcc
     1261 gccgttcagg accacgctgc ccgtgacctg ctgtccgttg acgaacgtgc cgttgcgcga
     1321 gccgagatcg gtcagctcga cgcgaccgtc cggaagcgcc ttcagggtgg cgtgccggcg
     1381 cgaggccttg gcgtcccgca ggacgaggtt cgtgttcccc tcgcggccga gcgtgaacag
     1441 gtcgccgctg acgttcgcgg tgcgccccgc gtcggcgccg gaggtgatct cgagtctcat
     1501 cgcgcgggca atctagctca caacgaagac ggcgacagcc tccgccacca gggtgtcgcc
     1561 ctcgcgcgcg ctcgcgcgag ttcggatgcg cccaccctcg cgcgtctcga cgctcgcctc
     1621 gacctcgagg aacgccccca caggcgccgg ggccagcagg tccagctcga gccggcgggt
     1681 gcgcgtatcg agcccctgcg cgtggagcgc gagcgccatc gcctcgtcga gcgcggtcgc
     1741 gacgatcccg ccgtgcgcgt acccgggcgg cccctggtgg tcctgcttga cgaagaaccg
     1801 cccccgcacg ccgtccgtct cgagctggag cccgaacagg ttcgcctgcc cgcagccgaa
     1861 gcagccgtcg tgatgcgtga tcaacgcagc cggttcagga cgaacggaag gatcccgccg
     1921 gccctgtagt actgccactc gttcggcgtg tcgatgcgga cggtggcctc cacggaagaa
     1981 ccatcgatgt cgatcgtcac ggttttctga ccatcctcga tctccggcac gccgatcacc
     2041 gtgtccccat ccacctcgac cgccgcctga agcggcagga tgcccatccc gacgaggttc
     2101 gatcggtgga tccgctcgta actctcggcg ataacgaacc gcacgccaag caaccgcggc
     2161 cccttcgcgg cccagtcacg cgaggacccc gacccgtact ccttgccggc gatcacgcac
     2221 agcggcacgc cctcgtcggc gtactccatc gccgcgtcga agatcggcag ctcgcgaccg
     2281 tccttgatcg tcacgccgcc ctcggtcccc ggtgccagca ggttccgcag ccgcacgttc
     2341 gcgaacgtgc cgcgcatcat cacctcgtgg tttccgcggc gcgagccgta ggagttgaag
     2401 tcctcgaccc cccgctccgt caggtaccgg cccgccgggg agtccttctt gatcgctccc
     2461 gcgggcgaga tgtggtcggt ggtcacgctg tcgccgagct tcgcgagcac tcgcgcaccc
     2521 tcgacggccc ccgcatacgg ctccatctcg aagtacggcg gccgcttcac gtacgtggac
     2581 tcgtcgtccc actcgtagcg atcgccctcg ggcacctgga tcgagttcca gttctcgtcg
     2641 ccctcgaaca cctcgccgta gctgcgcgtg aacatctcgc gttcgacgta ctgcatcgcc
     2701 gcggtcacct cggcctgcga cggccagatc tcgcgcaggg ggaagtcgtc gtagaggtcg
     2761 atgtccatgc ggcccgcaag cgcgtaggcc acgaccagcg gcggcgaggc gaggtagttg
     2821 agccgcacct cggggtggat gcgcccctcg aagttgcggt tgcccgacag gaccgagcag
     2881 acggccaggt cgcggtcgag gatctccttc gagatctcct ccgggagcgg gcccgagttc
     2941 ccaatgcagg tggtgcagcc gtagccgacg aggtcgaagc cgagcttgct gagcggctcg
     3001 aggagcccgg cgcgctcgag gtactcggtg acgaccttcg agccgggagc gagcgacgtc
     3061 ttgacccacg gcttgctctc gagccccatc tcaaccgcct tcttggccag cagcccggcc
     3121 gcgagcatca cgctcgggtt cgaggtgttg gtgcagctcg tgatggccgc gatgacgacg
     3181 gcgccgtggt cgaggccctc gccctggggc gtcttgtcgg caaccgcgac cacctcgctc
     3241 tcgcgcgaga cgtcctcgcg gccgttcgtc ggcgggtcgc tcgccgggaa gctctcactc
     3301 accgcctcgt cctggccctt gtactcgacc atctcgccga gggcctcgag ggcggacgcc
     3361 ttcgcctcgc gcagcggcac gcggtcctgc gggcgcttcg gcccggcgag cgacggctcg
     3421 acgtcgccga ggtcgagctc gagcgtgtcc gagtacgtcg cgtcgtcggc gccctcgtcg
     3481 tggaacatgc cctgctcgcg catgtatgcc tcgaccagct cggtgtcgcg gccggtgaac
     3541 tcgaggtagc ggagcgtctg gtcgtcgacc gggaagatcc cgcaggtcgc gccgtactcg
     3601 ggcgccatgt tgccgatcgt cgcgcggtcg gccagcggca gcgtcggcag gccggggccg
     3661 aagaactcca cgaacttgcc gacgaccccc ttcgcgcgca gcatctgcgt gacggtgagg
     3721 acgaggtcgg tggcggtcga tccctcaggc agctcccccg tgagccggaa cccgaccacc
     3781 tgcggcagca gcatcgagat cggctggccg agcatcgcgg cctcagcctc gatcccgccg
     3841 acgccccagc cgagcacgcc gagcccgttg accatcgtgg tgtgcgagtc cgtgccgacc
     3901 agcgtgtcgg ggaacgcctg gccgtcgcgc gtgtagacga cccgcgcgag gtactcgagg
     3961 ttgacctggt gacagatgcc cgtgttgggc ggaacgacgg cgaagtcgtc gaacgcggtc
     4021 tggccccagc gcaggaacgc gtagcgctcg ccgttgcgct cgaactcgcg ctcggcgttg
     4081 tgcacgaacg cgagcttggt ggcgaactcg tccacctgga tcgagtggtc gatcaccagc
     4141 tcggcgggca cgagcgggtt gatcttgctc gggtcgtcgg cggcgtcgcg catggcggcg
     4201 aggtccacga tcgcgggcac cccggtgaag tcctgcatga gcacgcgcgc gggctcgtag
     4261 gcgatctcct tgctcggctc ggccttcgcg tcccacgtcg cgatcgcctc gacggactcg
     4321 tcgtggccgt tgcgcagcgc gttctcgagc agcaccttca gcgagtacgg caggcgcgcg
     4381 acgtcgtact tcgcctggag ctcgtccagg cggaagatcc tgtaatcgcc gaggctagcc
     4441 atagttgatg ccgccgaacc agtgggtcgg gaggacaggc cactggcgcc tgaaccgcgc
     4501 cagctctgac gacgggaaga cctgcttcca gcgcgggtcg cgcgagtcgg ggtcggcgac
     4561 cgcgtgctcc ttgatcatcg agtcgtactg atcggaggag tcctcgagcg tgttcgagtt
     4621 catgacgacg tcgcggttga agaactcggc ccacttgcgc acgccgggga tcttcgacag
     4681 gtgcgggtgc cagttgtcgg cggcgacgcc gttctcgctc gagacccaga tcccgtccgg
     4741 cgtgttcacg cacagcgaca tgttcccgtc ggtgtggccg ggagtcgcga ggaacgcgag
     4801 ccccttgccg atctcgacat ccccctcgac caggaccagc ttgtcctcgg gaacgccgtc
     4861 catgccgccc tcgacgtacc aggcccactg ggtggggtgg atcgacctga acgtgtcgac
     4921 ctccgcgcgc tgggcgatca tcttcgcgtt cgggtagaag gccgactgcg tggcggtccc
     4981 gagcatcatg cgcggatcct gcacgtggag gtggtcgaac gcgatgtagt cgacgtcctc
     5041 gggggtcagg ccgcacttcg cgagcgcctg gccgatgtcg ttgtagtagg tcgcgaagac
     5101 gttgttggag agccactcgc cgtagcgctc gacgagctgc tcgtagaacg gcgcctcctg
     5161 cgagccctcg gggacggtcg gctcccaggc caggaccttc tcggcgccgt tgaagtcctc
     5221 gaagcggacg acgacgaggc ggttgctgat gctcacaaac gggttgagcc ccttcgcggc
     5281 gccgccgaac gcgaagttga gcgggtagcc ggcggcgacg aggtcgacgg tctggaccga
     5341 gagcacctgg ccctgctcct tgaagcgcgc ccggaactgc tcggcggcgg cgcggatcgc
     5401 ctgcggccgg tcgccgcggg gccagagaga ctgggcctcg ccgaattcgc cgaccgtgcg
     5461 cgcgaccgtc tccataaccg ccatggttag agcctattag ggtcgcctcg atgcacgaat
     5521 acgagcgcta cgagaacgct ttcggggacc gcgacgcgcc gttcgcgttc gtggacctgg
     5581 acgccatgtg ggccaacgcc gccgagatgc tcgggcgcgc gggcggcaag ccgatccggg
     5641 tggcgagcaa gtcggtgcgc tgccgcgagc tgctcgcgcg cgtgctggcg cgggacgggt
     5701 tcagcgggct gatgacctac acgctgcgcg agagccagtg gctcgacgag cacggcttcg
     5761 aggacctgct cctcgcgtat ccgacggccg accgtgaggc gctcgaccgc gagccgctgc
     5821 cggtgctgat ggtggactcg accgagcagc tcgacctgat cggtgatcgc catgcgcgcg
     5881 tgtgcatcga ggtggacctg agcttcccga tcgccgggcg gcggatcggg ccgaagcgct
     5941 cgccgatccg cacgccggcg caggcggtcg cgctcgcgcg aacggcgctc gcccgcggct
     6001 tcacgctcgt cggcgtaatg ggctacgagg gccacctggc gggcgtcggc gaccggcccg
     6061 agggccggct ccgggggatg gcgatccggg cgatgcagcg cgcgtcggtg cgcgagatgg
     6121 ccgagcggcg cgccgagatc gtggccgcgg tgcgtgaggt ggcgccgctc gagttcgtga
     6181 acggcggcgg caccggcagc ctgcacacga cctcgcgcga ggatgcggtc acggagctca
     6241 cggcgggctc cggcttctac gcccccgccc tgttcgactc ctactcggcg ttcacgctga
     6301 ggcccgctgc gatgttcgcg ctgccggtgg tgcggcggcc gagcgcgagg gtcgccacct
     6361 gcctcggcgg cggctacatc gcgtccggcg cggcggcggg cgatcggctc ccggtccccc
     6421 acctcccggc cggcctggag ctcgacgcgc aggagggcgc cggggaggtc cagacccccg
     6481 tgagcggggc cgcggcccgc cacatgcgcg tgggcgaccg cgtctacttc cgccacgcga
     6541 aggcgggcga gctgtgcgag cacttcgacg cgctgctgct ggtcgagggc gggcgcgtgg
     6601 tggacgaggt gccgacctat cggggcgagg ggaagttgtt cctgtagcga cgcggagtcg
     6661 tcacgttcgt caccttcgcg caacgcggtc cgtgcgagaa tgggcgcatg agcgagagcg
     6721 atctgcgcga gttcatacgc gagcagagcc tgatcgcgaa tcgccgaacc gacgccttcg
     6781 tgaaggagat cagcaggcag cgcgaggaga tgcgcctgca cttcgagaaa caggaccgca
     6841 agatcgacga gatggtggcc gagggcaagg ccgggcgcgc cgcgctgttc gcgatcctcg
     6901 atcggctcga caacggcggc cccgctaccg ccggttagtg cccgcgcaga tcctctacgg
     6961 gccgagcacg cggtccgtgt agtcgttgcg gaagcggcgg ttcgggtcga gccgctcgcg
     7021 cgccgcggcg aagcgctccc agtccgggta gtcgggcgcg aggtcggcgg cggttcggta
     7081 gtggcgcttg ccccagtgcg gccggccccc gtactcacgc atgatcgact cgaccgcgcg
     7141 gaagtacgtc tcgtgctccg tgccgcggta gacgtgcacc gcgacgtacg cggtcgcgcg
     7201 gccgttcgac ggcgacaggt gggcgtcgtc gggcgcgacg aagcggaact cgatcgggaa
     7261 cgagatcggc aggcggcggc gctcgatcat cgcgagcacg cgctcgagcg cctcgcccgc
     7321 gtgctcgcgc ggcaccgcgt actccatctc cgtgaagcgc accctgcgct ggttcgcgta
     7381 gatccggtag ctgcggtcga cccgctcaga cggctggagc atccgcgcga tggcgcggtt
     7441 catggcgggg atgccgcgcg gcagagcgcg gccggcgcgg ctgaacagct cgagcgcgag
     7501 gttctcgagc agcacgtcct gcacccactc cgccgccggc gccaggggtg cgggcggatc
     7561 ggtcgtggcc tcggtcacgc gggtgagggc gacccgcgtg taggggaaga cgaacagctc
     7621 gaagcgctcg tggccgtcga ccagctcgtc gagccgttcg agcgtctcgt ccagcggccg
     7681 cggggcgtcg aggcggcgca gggtgtagag cggcgcgcag cgcagcgtca cccgcgtgat
     7741 caccccgagc gcgcccaggc tcacgcgggc cgcgcggaag agctccggct cgtggccctc
     7801 cgagcagtcg accgcctcgc cgccgtcgcc gatcaggcgc aggccggcca cctgcgccga
     7861 caggttcgga aagcgcgcgc cggtgccgtg ggtggcggtg gcgatcgcgc ccgccagcga
     7921 ctgggggtcc acgtcgccct ggttctcgag cgcgagaccg tgctcggcga gggcggggcc
     7981 gagctcgcgg atgctggcgc cggcctcgat ctcggcgagc cccgtggacg cgtccgccgc
     8041 gagcacgcgg ttcattcggt gcagccgcag gaggtggccg tcggtcagca cgatgtcgct
     8101 gaacgaatgg cccgcgcccg cgacgcgcac gggccggccg gcgcgagcgg cgcgcccgat
     8161 cgccgcggcc acctgctcgt cgctcgccgg ctcctcgacc agctcgggag cgcagcgctg
     8221 gtcgccggtc cagttccgcc agggcgcggg catcgcgcga gaatacgtcc ggcaccccgc
     8281 ccggagcgac tgcgtaccct ccgttcgggg tacaacgagt acgaggggat cgtgaccggg
     8341 gaacacggaa gttcgcatcc ggcgcagctc agagccgccg cactggtggc cgccgcgctg
     8401 gcaggcacgt cgctggcggc gacggcggtt gccgacggca agccggctca gagcgagc
//
LOCUS       DASVPL010000083        20254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_323103, whole genome shotgun sequence.
ACCESSION   DASVPL010000083 DASVPL010000000
VERSION     DASVPL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 20254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20254
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323103"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VF517_03735"
     CDS             <1..744
                     /locus_tag="VF517_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052078.1"
                     /translation="EALPTQLVLAPEVAAPARGRFSEYGRDVAPVEGSAYRRRRRGVR
                     RPRGAVVLGADGVSYLRKGEEPVTVRFEEAVALIDDKPGSFSVKSRDATTVTVMPAEL
                     RGGDEVERTIRARVPEALWVPHAPNVAALTRLVGALPKPGRVGAELDELPDLLSPTET
                     PLALAEARRRRRRIGTVVATDRRIVWLYSGEDEEKVELPWEAVTAVRLDGRTLVVEGG
                     EEPLRLRRVRPASAAEAIARLAAERAAGD"
     gene            802..1035
                     /locus_tag="VF517_03740"
     CDS             802..1035
                     /locus_tag="VF517_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052079.1"
                     /translation="MGSVQTVVALAGGSFIFLGVALLMLVGVIYGFFTRAGSGIDQHP
                     SGKNAGAPGAVGRTEESGRDEGESSALETHGTR"
     gene            1113..1529
                     /locus_tag="VF517_03745"
     CDS             1113..1529
                     /locus_tag="VF517_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052080.1"
                     /translation="MRHAIRKQFYARLRPYLPFGRVIVAIAVAVGVATLTRQLLAQDE
                     DLYPAFLKALAAAFAVMVALGTGREFWRNKPVASAQGPAGTGGLGFGDESKATQQAVD
                     DLNTRVTTQMEDVNKRLFDLESAVFKPDDSGDDGGE"
     gene            1570..1746
                     /locus_tag="VF517_03750"
     CDS             1570..1746
                     /locus_tag="VF517_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052081.1"
                     /translation="MAREQNESNGRDGRSPAERYRDFARKNDDWDRKVKHSIEKIEKA
                     SRSGKRSERTISSN"
     gene            1825..2796
                     /locus_tag="VF517_03755"
     CDS             1825..2796
                     /locus_tag="VF517_03755"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX8052082.1"
                     /translation="MIGAPPRNRPCGGQYADIVEAIGHTPLVELRRLSPRPGVRIWAK
                     LESYNPTGSVKDRVAKSLIEDAESRGAIRPGQTILEPTSGNTGISLAMICSRKGYPLK
                     VVMPDNVTSERTQLLHMYGAEIVYSPGEQGSNGAVAKALEMAEADASYYMPYQYGNEA
                     NPLAHYNGTALEILEELDEVTAFVGGLGTGGTLMGNARRLKEENPDTKIVAAEPLQGE
                     LVQGLRSLEDGYIPPIIDISLLDRKIFVSNRDAVVWTKRLLEEEGLFLGVSSGAIAAI
                     ASRVANDLDEGNVVFVAPDDGWKYLSSGVYTKPIEELEATLDSASFW"
     gene            complement(2801..3124)
                     /locus_tag="VF517_03760"
     CDS             complement(2801..3124)
                     /locus_tag="VF517_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX8052083.1"
                     /translation="MVNVGPLEMMLVAIIALIVLGPARLPEVARSVGRGMREFRSALT
                     GAADYRDDDEDDDDDDEHELEPPDDDEDDDAPDDKGPNASPQAVEPAPVEPPPLPRTP
                     PGAAT"
     gene            complement(3140..3673)
                     /locus_tag="VF517_03765"
     CDS             complement(3140..3673)
                     /locus_tag="VF517_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052084.1"
                     /translation="MRVTRRSALAAAGAASLAAAAPARAADPARERAKRAVRAAVAGE
                     QVTAVAFEAIANSDAIADEHGETMQVLLDHAKAHATALAELFEAQTGEDAPLAPTRTK
                     IPGLDGLRGERQALRLALRLQEEAIALHLDAVRLYRNPGMLRLIAGTVGTDAQHLVLL
                     RSLLGREPVPEPFERGA"
     gene            complement(3670..4248)
                     /locus_tag="VF517_03770"
     CDS             complement(3670..4248)
                     /locus_tag="VF517_03770"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8052085.1"
                     /translation="MRTTYDLAIEKLPRRAALGLAAAALLAGCDGDDQDAQDTTQTIS
                     PERKRGDAAVLASLLDAERTAVFAYATARRRLGRLASRFLEHERAHARALENALADLG
                     VEPEPPRPRSAYATDFPPLRTREDALRFALDVEETQIAAYGDSMPALFTPEVRVTLTT
                     IFATQAEHVAVLLGELGEPQAPRDFVVGEPPE"
     gene            4247..4630
                     /locus_tag="VF517_03775"
     CDS             4247..4630
                     /locus_tag="VF517_03775"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX8052086.1"
                     /translation="MRISRAHHDELIAHAREEAPNECCGYARIDDGTIDAVFRGHNER
                     QSPYGFVMDGRSLLAANELDDDGFGVATYHSHPKSDPVPSEQDRNVAMYPHWLNLIVS
                     LKGEPHVRAWWIVDGDVREEPLDVD"
     gene            4599..5120
                     /locus_tag="VF517_03780"
     CDS             4599..5120
                     /locus_tag="VF517_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052087.1"
                     /translation="MCARSHSTSTDGLACPSCGRRYALDERFCDVCGMPLVHGGPARV
                     EEPRNEAHERARKIRPEYTRGELVRVAGGRNQAEAELIQGLLLEEGVPSMLRRTAGFD
                     VPDFLAAGPRDVMVPESGAARAHETLNDAQLGAAAFEEQSFRSRAVRIFAGLLLVGFA
                     GVLLALVLFQAAK"
     gene            5133..5825
                     /locus_tag="VF517_03785"
     CDS             5133..5825
                     /locus_tag="VF517_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052088.1"
                     /translation="MEGSGSGISRRVLLERAGLAGAGALLAQVPGFLSAKGWLEEAQA
                     QTADLSRDTLNGLVAFVVPGPDPYSVAQGQSTPEPGAIDAGATDQLIELLDRFVGVPA
                     GTLPASRAVSEVLNGYAAQVNPAAAGGAFPSHFARLSFDHKVEVFRRFESETEGSALR
                     FLSGILIGAVGFLAYNEGAVMDRGTRTLTSTPVGWTLSKYDGVAEGRPELRGYWKGRR
                     KVRTHPRYRVKR"
     gene            5822..7594
                     /locus_tag="VF517_03790"
     CDS             5822..7594
                     /locus_tag="VF517_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX8052089.1"
                     /translation="MRDVIVIGAGGGGPVIAKELAARGLDVLVLEAGPRNADPEREWS
                     HFENDANNPVTGYFRWGPSDRARSAWLRELPQNSFVWQLSGVGGTTQHYYGNCPRAYP
                     GVFRGYDGPDRNAYDRAHEFPFSYRKLIPYYEWVEHTLPVQTAPMGNKEMAWMRGASR
                     MGVPLQKTKDTLGAGYRPQENSILQSHGTAGRTSDQSKLVYPKARGCTFCGYCFQGCY
                     EPRNAPRNLIAKRSTDNSYVPMMLTADRWKKGGKAATLLTDAFATRIGTVDEGGATSA
                     RTVTWRVASTGEMVTEEAKVVVMAGGCTENPRLWLNSGLPNPNDWVGRGYTDHYFDWL
                     TGVMDDYTGSSKGPGSNVRMDYPGHGGLEQVGLPPGLQAFSATFSDGGINDVYLNGYD
                     RLPGVEGQGRRVGTALKSLLSDVDRLLNVLVITDDDVEPQNRVSLSSTLPPDEHGPVP
                     RIEFRYRERTKRTLENRRFMVERGVEMLRRAGAKQVYRINWAPLILHVQSTMRMGLDP
                     TNSVLDTKCESRAVKRLFVGDNSALPNALGGPNPTLTSQALATRTAEKIFHLYFDGDP
                     WVKRESPVSSISDRVTRAVIDRKL"
     gene            complement(7591..8274)
                     /locus_tag="VF517_03795"
     CDS             complement(7591..8274)
                     /locus_tag="VF517_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8052090.1"
                     /translation="MRRALVTGAARGIGAAIAERLRADGLEVVTLDRDAGCDIRADLA
                     RDALPDLGAIDVCVSNAAITDTIAPAHRMTEEQWRRDVDVNLSGAFRAVQACLAGMRE
                     RGFGRVIAISSGAAVAGLPGQVAYAASKAGLLGMVKTIAAENARRGITANAVLPGMVA
                     TEKVSAMPPDVLERVIAALPAGRMAEPAEVAALVAYLASGEAGYVNGQTIGIDGGLGL
                     NTLTLGSAP"
     gene            complement(8271..9809)
                     /locus_tag="VF517_03800"
     CDS             complement(8271..9809)
                     /locus_tag="VF517_03800"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX8052091.1"
                     /translation="MTTTAPERQIDRSGTRRLPPMTLLVFVVGAGSLGSEIAGARLLA
                     PYFGASTVIWANTIATVLTALSIGYWIGGRLADRRPDLAVMCRVVQCAAIATALVPLL
                     GRPFLGGAVEALDEVAAGAFLGSLLAVTVLLAVPILMMGTVAPFAIRLSVASIEESGQ
                     VAGRLYAISTIGSLAGVFASALLLIPLVGTQRTFLAFALAMSIVAATALPRRWWLVPL
                     GIAAALAIPTGGIKDEADNGRVIHESDTEYQYARVIEQPDGERDLELNEGVAIHSIYR
                     PGTYLTANYWDDTVVLPLAALDRPPRRMAILGNAAGTTARMYGHYFPQTSIDGVEIDG
                     ELTEIGRRYFDMRNPRLRTHTDDARPFLRRSEGGYDAIMVDAYRQPYIPFYLITEEFF
                     ELCRDRLADGGVVFVNVGHPDGNDDLEKVATAAMRRVFPHVIRDVTEDTNTLLLASER
                     EPSAQRLGEAGTRLPRELGEVAEEVSAQLEPPLTGGESYTDDRAPVEWLIDRSIVEYA
                     AGQP"
     gene            9890..10225
                     /locus_tag="VF517_03805"
     CDS             9890..10225
                     /locus_tag="VF517_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052092.1"
                     /translation="MSLFSKAAKFAKSPQGKKAMEKAKAFADKPETKEKIEQVREKVS
                     GKDKAQEASGSGDAAQAATSGSGSGSGPGQPGPEPSTPAAGGTPPINDPDAAAVGEQP
                     AQGNPPTAP"
     gene            complement(10373..10864)
                     /locus_tag="VF517_03810"
     CDS             complement(10373..10864)
                     /locus_tag="VF517_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052093.1"
                     /translation="MSDFDPQGLFERLGGAGIDYVVIGGWAVNAHGHRRLTADLDICP
                     SPARDNLERLARLLAELGAEHLGADEFERNEVPGDPTDPDSLALGGNFRLTTELGGLD
                     LMQWLTGLDTERAYERLASRAIEADVFGVRVRVCSLDDLLAMKRAAGRPVDREDIDAL
                     SAD"
     gene            complement(10861..11043)
                     /locus_tag="VF517_03815"
     CDS             complement(10861..11043)
                     /locus_tag="VF517_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052094.1"
                     /translation="MSPGPSTDARRLEREREHERRARREAVRLNAAKGPGRNLEEAIR
                     LARAADDFRTAFRPSR"
     gene            11106..11810
                     /locus_tag="VF517_03820"
     CDS             11106..11810
                     /locus_tag="VF517_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052095.1"
                     /translation="MAERWFSEEELRDMARPTMDRAIEAIDRGDLEEARVLCEAMKHE
                     WRHLHDLMVEGIGGLITFVQERFGEDAVAEAWEDGQGRGWTRDVEKIAARDRRQIVHA
                     LAATWRAHSCSGTGPHPGAFTIEEDDEKFIFRMNPCGSGQRLVRMGRYEGPNALGVTK
                     EAHDWSYGRAGFPLYCTHCSFMNESLPIRWIGYPLYPSDPPDDFGSDPCTWYWYKDPA
                     DIPARHWERHGLAKRT"
     gene            11849..12127
                     /locus_tag="VF517_03825"
     CDS             11849..12127
                     /locus_tag="VF517_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052096.1"
                     /translation="MADDDAEWVLPPAEPGDWHVFVSMDEEAQLSPRLKDAVKRLGEA
                     AEAENPKRYAAKPNNSCFNNWCGENEVSCFTLINPGDKCPRYDCPKMR"
     gene            12149..12391
                     /locus_tag="VF517_03830"
     CDS             12149..12391
                     /locus_tag="VF517_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052097.1"
                     /translation="MSDEQGAKWLVEPPQPGQIRVAISIPEETEVSDRLRSAVDEVIA
                     ALEDEEGVKTGDCWLDNVCSKNACGKQHWARTEGTG"
     gene            12391..13728
                     /locus_tag="VF517_03835"
     CDS             12391..13728
                     /locus_tag="VF517_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8052098.1"
                     /translation="MRESAYNMWVEHGEDDYVFNGVSGGLLRVPREERARLEAFLGEE
                     SDDCSPDLLERLARGRMLIGDETDELALLSRRYHGTRWNRGRLGLTVVTSLGCNFDCP
                     YCFEDKHHSLLSSEVEAAIIGIVDEQIPQLDHVAVTWYGGEPLLGKRALFRLSDELIA
                     RCDAADVAYSGSIVTNGYLLDEATCEELASRRIDNAQVTIDGPPDVHNKMRPLVSGGK
                     SFDRIVANLKAAVRHMSIAVRVNLDTSNVHRVEELMKILADEGLSGKLGVYAAQLTQV
                     DDGAPAPSASYATACFSCGEFSEVQSEFRELAESYGFDSPSLPGPSGAPCTAVRANEL
                     VIGSKGELYKCWDSVGNPAEVVGHIRDYRKAETRAAKWLSYDPFENDEWRSCIALPVC
                     MGGCAHHGMDEKLYDDRCMPFRWNYAEQVRSFVKANADGTAEPVVLSAAGHTR"
     gene            13765..14586
                     /locus_tag="VF517_03840"
     CDS             13765..14586
                     /locus_tag="VF517_03840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8052099.1"
                     /translation="MLRRSAIAVAAVLAALAAAPPAGASLVALGDSFSSGEGAPPFEP
                     GHCHRSPHAWPLVLAARLDAPGTSRACSGAVVADVVQDQIPRVAPGADVLTLTIGGND
                     LGFAEVLRRCVLADCVRHYARAGRDAIDARVRALRARLPALYRRVRSAAPGAGLVVLG
                     YPRLFPRRPGRFTCAALGAISRAEALYLNAKTAAADRAIRAAARDAGALYVETLDAFD
                     GGEVRCAPRRRYVNDLTFLRGGVISPYSFHPTAAGHARLAALALDGLAANVRDRR"
     gene            14598..15872
                     /locus_tag="VF517_03845"
     CDS             14598..15872
                     /locus_tag="VF517_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8052100.1"
                     /translation="MDFSPSPRVEELKGRIDAFLDEHYYPHELDIFNELDEEVGPGVA
                     YPKRLVELRERAKAEGLWNLFLPGEEHGPGLTNWEYGMICEAMGRSPIAPMVFNCSAP
                     DTGNAEILVDHGTPEQKQEWLEPLLEGTIRSCFSMTEPETAGSDPTGLRGTAVLDGDE
                     WVIDAHKWFTSGAVGASVAIVMVVTDPDAAPHKRASMILVPTDAPGFNLVRPVSVMGH
                     SGGPGHCEIRYESCRVPKDNLLGERGAGFKIAQDRLGPGRIHHCMRAIGAAERAHEMM
                     CRRATERHAFGGPLAEKQFVQDFIAKSRIEIDGARLMVLNAAWQMDELGKREARQAIS
                     MTKVVAANVVMDVLDRAIQVHGALGVSDDTPLAAMWRGLRMLRLADGPDEVHKMVIAL
                     RELNRYRDKEPSGGGNGLAPVQPPAGVGSGAS"
     gene            15875..17041
                     /locus_tag="VF517_03850"
     CDS             15875..17041
                     /locus_tag="VF517_03850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8052101.1"
                     /translation="MLDATLTRNNDIALSDEQQDFVRSIRDFCQREFPKDKLEQLTDG
                     FKDIHNDDVARKMAELGWFGLTIDEEYGGSGGTFLDAALFLEESAHGRAPVSGYPVTL
                     IVVGALNRFGTEEQKRDLLGRVAAGGGLAIAMSEPESGSDVASLKTKARRENGEWVLS
                     GSKMWCSYAHKASHTLIVCRTGEGSSNHEGLSMIFVPRDAEGFTITPIPTLGGEETNE
                     LYLDDVRVPEENLLGTEGNGWTQLMAGLNNERVILGAGALGLAQRAFDDALAYAKERK
                     QFGRPIGGFQAIQHKFADMATGLAQARLLVRWVARLTDEDAGRMLPQEASRVKLACTE
                     LAKKCTLEGVQIMGGYGYATEYPMERYLRAAVVTTIYGGTSEIQKNIIAKTLGL"
     gene            17218..18852
                     /locus_tag="VF517_03855"
     CDS             17218..18852
                     /locus_tag="VF517_03855"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX8052102.1"
                     /translation="MTAVSACSSAYAQVPDLPQAPRLPDTPVTQPAQNVADQASDTAN
                     QVTGQVNQTVNPQGGGSQPSGGGGGGTGGGGGGGNMPSGNGGGSSGGGSNSGDGGSDG
                     GGDNGSSRGGSTAAERRADRRARTRAAARRAERRRERTAARGDEGEDRPSRDGDNGPG
                     PLRPIRDVVEVIPVPMWIALGLLALLAALFYVRGFLTARRARQLEAQRSELLDDVGLL
                     QKALLPDVPERIGGLEASVAYRPADGPAAGGDFYDVFEMDAGRVGIIVGDVCGHGRQA
                     LAVTALMRYTLRAYLNAGGEPRAALQIAARALENEQHAELTTVVLAVYDAKAGTLTYA
                     CAGHEPPILVGPAAHKPVTVGSSPPIGAGLVTGLRQTTVALPAGSLACFFTDGLVEAR
                     LDDDALLGRDRLCTIVEQLGDGACADALLAKIASDAERAPDDMAACIIRAAADAPVVS
                     DVRIEEFELDDNGVGEERARGFLAACGVPESEADTVIKTARGARAEFGGAMLRVRLSG
                     SGGGVKVVPLELRPLAPSTNGHHPPAVVPVAPPISA"
     gene            18936..19262
                     /locus_tag="VF517_03860"
     CDS             18936..19262
                     /locus_tag="VF517_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052103.1"
                     /translation="MRKLPIAVVLAVVASLAFSSVASAHTLRYNTAKNLAVKLAKKQE
                     RNDPVKRWRIFGAERHSDHEISWTYNVDYDDGSLCDAELVVRYTTKSNGKPIVRAFFR
                     HVECTR"
     gene            complement(19259..20008)
                     /locus_tag="VF517_03865"
     CDS             complement(19259..20008)
                     /locus_tag="VF517_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052104.1"
                     /translation="MPWIESHSPSFSARHEERDTDAAAAMLEQLERFRDELDDAFGST
                     PGEVAVIMHPGPLALSLAHPWLPVARMAAAPAARRYMAGWFGRREIHVLAPAALEERA
                     SAVPGSREALLLSPLHEYAHVVIGANNTTLPPPFTPRTFARYLRWAWLCEGAATHLAG
                     QTKHLRPAIARRLREGGRPAFPPQARDAVLLGGTVFAMLEDELGPEECVRLAARPPEA
                     GDAAAIQRAFGRPASEVEADWRRRLDELRAS"
     gene            complement(20058..>20254)
                     /locus_tag="VF517_03870"
     CDS             complement(20058..>20254)
                     /locus_tag="VF517_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052105.1"
                     /translation="VNGKRRLRVKGKDVRRIALEKLPQKRFVVRIRAVHSNGTEIIST
                     RVYKGCKQSKPKTTRHGPKR"
BASE COUNT         2993 a         7010 c         7473 g         2778 t
ORIGIN      
        1 gaggcgctgc cgacgcagct cgtgctggcg cccgaggtcg cggcgcccgc gcgcgggcgg
       61 ttctccgagt acgggcgcga cgtggcgccg gtcgagggct ccgcgtatcg caggcggcgc
      121 aggggcgtgc gccgcccgcg cggggcggtc gtgctcggcg cggacggggt gagctacctg
      181 cgcaaggggg aggagccggt gaccgtgcgg ttcgaggagg ccgtcgcgct gatcgacgac
      241 aagccggggt cgttctcggt gaagtcgcgc gacgcgacca cggtgaccgt catgccggcg
      301 gagctgcgcg ggggcgacga ggtcgagcgg acgatccgcg cgcgcgtgcc ggaggcgctg
      361 tgggtgccgc acgcgccaaa cgtcgcggcg ctcacgcggc tcgtcggggc gctcccgaag
      421 ccggggcgcg tgggcgctga gctggacgag ctacccgacc tgctcagccc gaccgagacg
      481 ccgctcgcgc tcgccgaggc ccgccgccgc cggcgccgga tcgggaccgt ggtggcgacc
      541 gaccggcgca tcgtctggct ctactccggc gaggacgagg agaaggtgga gctgccgtgg
      601 gaggccgtca cggcggtgcg cctcgacggc cgcacgctgg tggtcgaggg cggcgaggag
      661 ccgctgaggc tgcggcgcgt ccggccggcc agcgccgcgg aggcgatcgc gcggctcgcc
      721 gccgagcgag ccgccggcga ctgatccggg tcccgccggg gtgcgaatag ccccaaaggg
      781 ctacgcaggg aggcgcggat tatgggttcg gttcagacgg tggtcgcgct ggcgggcggc
      841 tcgttcatct tcctgggagt cgcactgctg atgctggtcg gggtcatcta cggcttcttc
      901 acgcgagcgg gcagcggtat cgaccagcat ccgagcggca agaacgccgg ggcaccgggc
      961 gccgtcgggc gcaccgagga gtccgggcgc gacgagggcg agagcagcgc cctcgagact
     1021 cacggcacgc gctaggagcg gtcaccgcgg ccacgcgcga tcgtgccagt gtgaaccgga
     1081 ggcacgtccg tcggcgcgct tagcctgggc tagtgcgcca cgcgatccgg aagcagtttt
     1141 acgctcgact gcggccgtat ctgccattcg gccgcgtgat cgttgccatc gcagtggcgg
     1201 tcggggtggc gaccttgacg cggcaactcc tggcccaaga cgaggatctc taccccgcat
     1261 tcttgaaagc tcttgctgcg gcgttcgcag tcatggtcgc cctcggtacc gggcgcgagt
     1321 tctggcggaa caagccggtg gcttcggccc aggggcctgc cggaaccggg gggctcggct
     1381 ttggcgacga atcgaaggcg acccagcagg cggtggacga cttgaatacg cgggtgacga
     1441 cccagatgga ggacgtgaac aagcggctgt tcgatctcga atccgccgtg tttaagccgg
     1501 acgactcagg ggacgacggg ggagagtagg ctttctccac ttggattccc ttttgaaagg
     1561 gagcttggga tggcacgcga gcagaacgaa agcaacggcc gcgacggtcg ttcccccgct
     1621 gagcgatacc gcgacttcgc gaggaagaac gacgactggg accgcaaggt caagcactcg
     1681 atcgagaaga tcgagaaggc gtctcgctca ggcaagcgca gcgagcgcac catctccagt
     1741 aactagtccg agcgggtaga ccgctcctgg gtggcggaat cgatagggcc gccgccgcgc
     1801 gcgggtcact acactttctg aagcatgatc ggggcgcctc ctcgaaaccg tccgtgcggc
     1861 gggcagtacg ccgacatcgt cgaggccatc ggccacacgc ccctggtcga gctcaggcgc
     1921 ctctcgccgc ggcccggcgt ccggatctgg gccaagctcg agagctacaa cccgaccggc
     1981 tcggtcaagg acagggtcgc gaagtcgctg atcgaggacg ccgagtcgcg cggggcgatc
     2041 cgtcccggcc agacgatcct cgagcccacc tccggcaaca ccggcatctc gctggcgatg
     2101 atctgctcgc gcaagggcta ccccctcaag gtggtcatgc ccgacaacgt cacctccgag
     2161 cgcacgcagc tcctgcacat gtacggcgcc gagatcgtct actcgccggg cgagcagggg
     2221 tcgaatggcg cggtggccaa ggcgctcgag atggcggagg ccgacgcgtc gtactacatg
     2281 ccctaccagt acggcaacga ggcgaacccg ctggcccact acaacggcac cgccctggag
     2341 atcctcgagg agctcgacga ggtcaccgcg ttcgtgggcg gcctcggcac cggcggcacg
     2401 ctgatgggca acgcgcgccg gctcaaggag gagaaccccg acaccaagat cgtcgccgcc
     2461 gagccactcc agggcgagct cgtccagggc ctccgctcgc tcgaggacgg ctacatcccg
     2521 ccgatcatcg acatctcgct gctcgaccgc aagatcttcg tctcgaaccg cgatgcggtg
     2581 gtctggacca agcggctgct cgaggaggag ggcctgttcc tgggcgtctc gtcgggcgcg
     2641 atcgccgcga tcgcctcgcg cgtggccaac gacctcgacg agggcaacgt cgtcttcgtc
     2701 gcgcccgacg acggctggaa gtacctcagc tccggcgtct acacgaagcc gatcgaggag
     2761 ctcgaggcga cgctcgactc cgcgtcgttc tggtaggtcc ctaggtcgcg gcgcccggcg
     2821 gggtgcgcgg aagcggcggt ggttcgactg gcgccggctc gacggcctgc gggctcgcgt
     2881 tcggcccctt gtcgtccggc gcgtcgtcgt cctcgtcgtc gtccggcggc tcgagctcgt
     2941 gctcgtcgtc gtcgtcgtcg tcctcgtcgt cgtcgcggta gtccgcggcg cccgtcagcg
     3001 ccgagcggaa ctcgcgcatg ccgcgcccga ccgagcgcgc gacctcgggc agccgggcgg
     3061 ggccgagcac gatcagcgcg atgatcgcga cgagcatcat ctcgaggggg ccgacgttga
     3121 ccattcagcc agggtagtcc taggcgccgc gctcgaacgg ctcgggcacc ggctccctgc
     3181 cgagcagcga gcgcaacagc accaggtgct gggcgtccgt gccgacggtg ccggcgatca
     3241 gtctgagcat cccggggttg cgatagaggc gcacggcgtc gaggtgcagg gcgatcgcct
     3301 cctcctggag ccgcagcgcg agccgcagcg cctggcgctc gccgcgcagg ccgtcgagcc
     3361 cgggaatctt cgttcgcgtg ggggcgagcg gggcgtcctc gccggtctgc gcctcgaaaa
     3421 gctccgcgag agcggtcgcg tgcgccttcg catggtcgag cagcacctgc atcgtctcgc
     3481 cgtgctcgtc ggcgatcgcg tcgctgttgg cgatcgcctc gaacgcgact gccgtgacct
     3541 gctcgccggc caccgcggcg cgcaccgcgc gcttcgcccg ctcgcgcgcg gggtccgccg
     3601 cgcgcgccgg cgcggccgcg gcgagcgacg cggcgccggc ggccgccagc gcagatcggc
     3661 gcgtaaccct cactccggcg gctccccgac cacgaagtcg cgcggcgcct gcggctcgcc
     3721 gagctccccg agcagcaccg cgacgtgctc ggcctgggtg gcgaagatcg tggtgagcgt
     3781 gacgcgcacc tccggcgtga agagcgccgg catcgagtcc ccgtacgcgg cgatctgcgt
     3841 ctcctccacg tcgagcgcga agcgcagcgc gtcctcgcgc gtgcgcagcg gcgggaagtc
     3901 ggtcgcgtac gcggagcgcg gtcgcggcgg ctcgggctcc acgccgaggt cggcgagcgc
     3961 attctcaagc gcccgcgcgt gcgcgcgctc gtgctcgagg aaccggctcg cgaggcggcc
     4021 gagccgccgg cgcgcggtgg cgtacgcgaa cacggcggtg cgctcggcgt cgagaagcga
     4081 ggcgagcacc gcggcatcgc cgcgcttgcg ctcggggctg atcgtctggg tggtgtcctg
     4141 cgcgtcctgg tcgtcgccgt cgcaccctgc cagcagggcg gcggcggcga gcccgagagc
     4201 ggcgcggcgg gggagcttct cgatggccag atcgtatgtt gtccgcatgc ggatctcgcg
     4261 ggctcaccac gacgagctga tcgcgcacgc gcgcgaggag gcgccgaacg agtgctgcgg
     4321 ctacgcgcgc atcgacgacg ggacgatcga cgcggtgttc cgcggccaca acgagcgtca
     4381 gtccccgtac gggttcgtga tggacggcag gagcctgctc gccgcgaacg agctcgacga
     4441 cgacggcttc ggggtcgcca cctaccactc gcacccgaag agcgacccgg tcccctcgga
     4501 gcaggaccgc aacgtcgcca tgtacccgca ctggctgaac ctgatcgtgt cgctcaaggg
     4561 ggagccgcac gtgcgcgcgt ggtggatagt ggacggggat gtgcgcgagg agccactcga
     4621 cgtcgactga cgggctggcc tgcccgtcgt gcgggcgccg ctacgcgctc gacgagcgct
     4681 tctgcgacgt gtgcggaatg ccgctcgtcc acggcggtcc cgcacgcgtg gaggagccgc
     4741 gcaacgaggc ccacgagcgc gcgcgcaaga tccgcccgga gtacacgcgc ggcgagctcg
     4801 tccgcgtggc cggcgggcgc aaccaggccg aggccgagct gatccagggg ctgctgctcg
     4861 aggagggggt gccgagcatg ctccgccgca ccgcggggtt cgacgtgccc gacttcctcg
     4921 cggccgggcc gcgcgacgtg atggtgcccg agtcgggtgc cgcacgcgcg cacgagaccc
     4981 tcaacgacgc gcagctcgga gccgccgcgt tcgaggagca gtcgttccgc tcacgcgcgg
     5041 tgcgcatctt cgccgggctg ctcctggtcg gcttcgccgg cgtgctgctc gcgctggtgc
     5101 tgttccaggc cgcgaagtag taccgtcgcg ccatggaggg gagcgggagc gggatctcgc
     5161 gtcgcgtgct gctcgagcgc gccggtctcg ccggtgcggg ggcgctgctc gcccaggtgc
     5221 cggggtttct ctcggcgaag ggctggctcg aggaggccca ggcgcagacg gccgacctct
     5281 cgcgcgacac gctcaacggc ctcgtcgcgt tcgtcgtgcc cggcccggac ccgtactccg
     5341 tcgcgcaggg gcagagcacc cccgagcccg gggccatcga cgcgggagcg accgaccagc
     5401 tgatcgagct gctcgaccgc ttcgtgggtg tgccggcggg cacgctgccg gcgtcgcgcg
     5461 ccgtgtcgga ggtcctcaac ggctacgcgg cgcaggtgaa ccccgcggcg gcgggcggag
     5521 cgtttccgtc gcacttcgcg cggctgagct tcgaccacaa ggtggaggtt ttccgccgct
     5581 tcgagagcga gacggagggc tccgcgctgc gcttcctctc cgggatactg atcggcgcgg
     5641 tcggcttcct cgcctacaac gagggtgccg tgatggacag gggcacgcgc acgctcacct
     5701 cgaccccggt gggctggacc ctgtcgaagt acgacggggt ggccgagggc cggccggagc
     5761 ttcgggggta ctggaagggc cgccggaagg tgcgcaccca cccgcgctac cgggtgaagc
     5821 gatgagggac gtgatcgtga tcggcgcggg cggcggcggg cccgtgatcg ccaaggagct
     5881 ggccgcgcgc gggctcgacg tgctggtgct ggaggccggg ccgcgcaacg cggaccccga
     5941 gcgcgagtgg agccacttcg agaacgacgc gaacaacccg gtgaccggct acttccgctg
     6001 ggggcccagc gaccgcgcgc gctcggcgtg gctgcgcgag ctgccgcaga acagcttcgt
     6061 gtggcagctc tcgggcgtcg gcggcacgac gcagcactac tacggcaact gcccgcgcgc
     6121 gtatcccgga gtgttccgcg gctacgacgg tcccgaccgc aacgcctacg accgcgcgca
     6181 cgagttcccg ttctcgtacc gcaagctgat cccgtactac gagtgggtcg agcacacgct
     6241 gcccgtgcag accgccccga tgggcaacaa ggagatggcc tggatgcggg gcgcgagccg
     6301 gatgggcgtt ccgctccaga agacgaagga cacgctcgga gcgggctacc ggccgcagga
     6361 gaactcgatc ctccagtcgc acggcaccgc ggggcgcacc agcgaccaga gcaagctcgt
     6421 ctatcccaag gcgcgcggct gcacgttctg cggctactgc ttccagggct gctacgagcc
     6481 gcgcaacgcc ccgcgcaacc tgatcgccaa gcgctccacc gacaacagct acgtgccgat
     6541 gatgctgacc gccgaccgct ggaagaaggg cggcaaggcg gcgaccctgc tcaccgacgc
     6601 gttcgcaacc cgcatcggca cggtcgacga gggcggggcc acgagcgcgc gcacggtcac
     6661 ctggcgggtg gccagcacgg gcgagatggt caccgaggag gcgaaggtcg tggtgatggc
     6721 cggcgggtgc acggagaacc cgcggctgtg gctcaacagc gggctgccga acccgaacga
     6781 ctgggtcggt cgcggctaca ccgaccacta cttcgactgg ctgaccggcg tgatggacga
     6841 ctacacgggc tcgtccaagg gacccgggag caacgtccgg atggactacc cgggccacgg
     6901 cgggctcgag caggtggggc tcccgcctgg gcttcaggcc ttctcggcga cgttctccga
     6961 cggcggcatc aacgatgtct acctcaacgg ctacgaccgg ctgcccgggg tggaggggca
     7021 ggggcgccgc gtcgggaccg cgctgaagtc gctcctctcc gacgtcgacc ggctgctgaa
     7081 cgtcctggtg atcaccgacg acgacgtcga gccacagaac cgcgtctcgc tctcgagcac
     7141 gctgccgccg gacgagcacg ggccggtgcc gaggatcgag ttccgctacc gcgagcggac
     7201 caagcggacg ctcgagaacc gccgcttcat ggtcgagcgc ggggtcgaga tgctccggcg
     7261 cgcgggggcg aagcaggtct accggatcaa ctgggccccg ctgatcctgc acgtgcagtc
     7321 gaccatgcgt atggggctcg accccacgaa ctcggtgctc gacaccaagt gcgagtcgcg
     7381 cgccgtgaag cgcctgttcg tgggcgacaa cagcgcgctg ccgaacgccc tcggcggccc
     7441 gaacccgacg ctcacgtcgc aggcgctcgc cacccgcacg gcggagaaga tcttccacct
     7501 ctacttcgac ggcgacccgt gggtgaagcg cgagagcccg gtctcgtcga tcagcgaccg
     7561 cgtgacgcgg gccgtgatcg accgcaagct ctagggcgcc gagcccaggg tcagcgtgtt
     7621 gaggccgagg ccgccgtcga tgccgatggt ctggccgttc acgtagccgg cctcgccgga
     7681 ggccaggtat gccacgagcg cggccacctc cgccggctcc gccatgcgcc cggcgggcag
     7741 cgccgcgatc acgcgctcga gcacgtccgg cggcatcgcg ctcaccttct cggtggcgac
     7801 catgccgggc aggaccgcgt tggccgtgat cccgcggcgc gcgttctcgg cggcgatcgt
     7861 cttgaccatg ccgagcagcc cggccttgga cgccgcgtac gcgacctggc cagggagccc
     7921 ggccaccgcg gcgccgctcg agatggcgat cacccggccg aagccgcgct cgcgcatccc
     7981 cgccaggcag gcctggaccg cgcggaaggc gccgctcagg ttcacgtcga cgtcgcgccg
     8041 ccactgctcc tctgtcatgc ggtgcgcggg agcgatcgtg tcggtgatcg cggcgttcga
     8101 gacgcagacg tcgatcgcgc cgaggtcggg gagcgcgtcg cgcgcgaggt cggcccggat
     8161 gtcgcagccc gcgtcgcggt cgagcgtgac cacctcgagc ccgtccgccc gcagccgctc
     8221 ggcgatggcg gcgccgatgc cccgcgcggc gccggtcacg agcgcgcgcc tcacggctgg
     8281 cccgccgcgt actccacgat cgagcggtcg atcagccatt cgacgggggc gcggtcgtcg
     8341 gtgtaggact cgccgcccgt gagtggcggc tcgagctggg ccgagacctc ctcggcaacc
     8401 tcgccgagct cgcgcggcag tcgcgtgccc gcctcgccga gccgctgcgc cgagggctcg
     8461 cgctcggagg cgagcagcag ggtgttcgtg tcctcggtga cgtcgcggat cacgtgcggg
     8521 aagacccttc gcatggccgc ggtcgcgacc ttctcgaggt cgtcgttccc gtcggggtgg
     8581 ccgacgttga cgaacaccac gccgccgtcc gcgagccggt cgcggcagag ctcgaagaac
     8641 tcctcggtga tcaggtagaa cgggatgtac ggctgacggt acgcgtcgac catgatcgcg
     8701 tcgtagccgc cctcgctgcg acgcaggaac ggcctagcat cgtcggtgtg cgtgcgcagg
     8761 cgcgggttgc gcatgtcgaa atacctgcgc ccgatctcgg tcagctcgcc gtcgatctcg
     8821 acgccgtcga tcgacgtctg cgggaagtag tgaccgtaca tgcgcgccgt ggtgccggcc
     8881 gcgttgccga ggatcgccat gcgccgcggc gggcggtcga gcgcggccaa ggggagcacg
     8941 accgtgtcgt cccagtagtt ggcggtcagg taggtgccgg gacggtagat cgagtggatt
     9001 gccacgccct cgttcagctc gagatcgcgc tcgccgtcgg gctgctcgat cacccgcgcg
     9061 tactggtact cggtgtcgct ctcgtggatc acgcggccgt tgtcggcctc gtccttgatg
     9121 ccgccggtgg ggatcgccag cgcagccgcg atgccgagcg ggacgagcca ccagcgccga
     9181 ggcagtgccg tggcggcgac gatcgacatc gccagcgcga aggcgaggaa cgtgcgctgc
     9241 gtgccgacca gcgggatcag caatagcgcc gaggcgaaca cgcccgcgag cgacccgatc
     9301 gtcgagatcg cgtagagccg gccggccacc tggccgctct cctcgatcga cgcgaccgac
     9361 agccggatcg cgaacggcgc gacggtgccc atcatcagga tcgggacggc cagcaggacg
     9421 gtgacggcga gcagcgaccc gaggaatgcg ccggcggcga cctcgtcgag ggcctcgacc
     9481 gcaccgccga ggaacggacg accgagaagc ggaacgagcg cggtggcgat ggcggcgcac
     9541 tggacgacgc ggcacatgac ggcgaggtcg gggcggcggt cggccagccg tccgccgatc
     9601 caatagccga tcgacagcgc cgtgagcacc gtggcgatcg tgttcgccca gatgaccgtg
     9661 gacgcgccga agtacggcgc aagcaggcgc gcgcccgcta tctcgctgcc gagcgagccg
     9721 gcgccgacga cgaataccag gagcgtcatg ggcggcagcc ggcgcgtgcc ggagcggtcg
     9781 atctgcctct ccggagcggt cgtcgtcacc cgattggcca tgctaccggc gccgcttcgc
     9841 cgctcggggg cgccgcttcg gcgtgcgagc cgctgggtag gctcggcgca tgtccctctt
     9901 ttcaaaagca gcgaagttcg caaagagccc tcagggcaag aaggcgatgg agaaggccaa
     9961 ggcgttcgcc gacaagccgg agaccaagga gaagatcgag caggtgcggg agaaggtgtc
    10021 cgggaaggac aaggcgcagg aggcgtcggg cagcggggac gcggcgcagg ccgcgaccag
    10081 cggctccggt tctggttccg gtcccggtca gcccgggcct gagccgagca cgccggccgc
    10141 cggcggtacg ccgcccatca acgatccgga tgccgccgct gtcggtgagc agccggcgca
    10201 ggggaatcct ccgaccgctc cctaacggct gcggttcgtg acgccgccgc ggggcggcgc
    10261 gtgcacatag ttcctcgggg gtacggtcgc aggctccctc agcgagcccc cctcggaact
    10321 acgtgcacgc gccgccgtca tcgtgtgcgt cctccggcgc tctgctacgc ggtcagtcgg
    10381 cgctgagtgc gtcgatgtcc tcgcggtcga ccgggcgtcc ggccgcgcgc ttcatcgcga
    10441 ggaggtcgtc cagtgagcag acgcggaccc tgacgccgaa gacatcggcc tcgatcgctc
    10501 tcgacgcgag ccgttcgtaa gcgcgctcgg tgtcgagacc ggtaagccat tgcatcaggt
    10561 cgagtccgcc cagttccgtg gtcaagcgga agttgccgcc gagcgcaagg ctgtccggat
    10621 cagttgggtc accggggacc tcgttgcgct cgaactcgtc ggcgccgagg tgctcggcgc
    10681 ctagctccgc gagcagccgc gcaagtcgct cgaggttgtc gcgcgccggg ctcgggcaga
    10741 tgtcgaggtc ggccgtgagg cgcctgtgcc cgtgcgcgtt gacggcccag cctccgatga
    10801 cgacgtagtc gatgcccgcg ccgccgagcc gctcgaagag cccctgcgga tcgaagtcgc
    10861 tcaacggctg gggcggaagg cggtgcggaa gtcgtccgcg gcacgcgcga ggcggatcgc
    10921 ctcttcgaga ttgcgtcccg gtcctttggc tgcgttgagt cgcaccgcct cccggcgcgc
    10981 acggcgctcg tgctcgcgct cccgctcgag gcgtcgggcg tcggtcgacg gtccgggcga
    11041 catgcacgga atgctacgcc tgcgatcagc tgtagcgtcc ccgcgcgggc gagctaggag
    11101 gagctatggc ggagcggtgg ttcagcgagg aggagctgcg ggatatggcg cggccgacta
    11161 tggaccgggc catcgaggcg atcgatcggg gggacctcga ggaggcgcga gtgctctgcg
    11221 aggcgatgaa gcacgagtgg cgtcatctcc acgacctgat ggtcgagggg atcggcgggc
    11281 tcatcacgtt cgtgcaggag cgcttcggtg aggacgccgt cgcggaggcg tgggaggacg
    11341 ggcaggggcg cggctggacg cgcgacgtcg agaagatcgc cgcccgcgac cgccggcaga
    11401 tcgtccacgc gctcgccgcg acctggcgag cgcactcgtg cagcggcacc ggcccgcacc
    11461 cgggcgcttt cacgatcgag gaggacgacg agaagttcat cttccggatg aacccgtgcg
    11521 gctccggcca gcggctcgtg cgcatggggc gctacgaggg gccgaacgcc ctcggggtga
    11581 cgaaggaggc gcacgactgg agctacggcc gcgcgggctt cccgctctac tgcacccact
    11641 gctcgttcat gaacgagtcg ctcccgatcc gctggatcgg ctacccgctc tacccgagcg
    11701 acccgccgga cgacttcggc agcgacccgt gcacctggta ctggtacaag gatccggccg
    11761 acatccccgc gcgccactgg gagcggcacg ggctggccaa gaggacatag cgctggacat
    11821 gtattgacgt gccggttagt gtcgcgccat ggcggacgac gacgccgagt gggtcctgcc
    11881 tccggcggag cccggcgact ggcacgtttt cgtttcgatg gacgaggagg cgcagctcag
    11941 cccgcgcctc aaggacgccg tgaagcgcct tggcgaggcg gcggaggcgg agaatcccaa
    12001 gcgctacgcc gcgaagccca acaacagctg cttcaacaac tggtgcgggg agaacgaggt
    12061 ctcctgcttc acgctgatca acccgggcga taagtgcccg cgttacgact gcccgaagat
    12121 gagatgaacg acgagggagg gagggcccat gtccgatgag caaggggcga agtggctggt
    12181 ggagccgccg cagcctggtc agatcagggt ggcgatctcg attcccgagg agaccgaggt
    12241 gagtgaccgg ttgcgctcgg cggtcgacga ggtgatcgcg gcgctcgagg acgaggaggg
    12301 cgtgaagacc ggcgactgct ggctcgacaa cgtctgttcc aagaacgcct gcggcaagca
    12361 gcactgggcg cgcacggagg ggaccggcta gtgcgcgagt ccgcgtacaa catgtgggtc
    12421 gagcacggcg aggacgacta cgtcttcaac ggggtgagcg gagggctgct gcgggtgccc
    12481 agggaggagc gggcccgcct ggaggcgttc ctcggggagg agagcgacga ctgctcgccc
    12541 gacctcctcg agcggctggc ccgcggccgc atgttgatcg gagacgagac ggacgagctc
    12601 gcgctgctgt cgcgccgcta ccacggcacg aggtggaatc gcggccggct cgggctgacg
    12661 gtggtcacga gcctcggatg caacttcgac tgcccgtact gcttcgagga caagcaccac
    12721 tcgctgctgt cgagcgaggt ggaggcggcg atcatcggca tcgtcgacga gcagatcccg
    12781 cagctcgacc acgtggccgt cacgtggtac ggcggtgagc cgctgctcgg caagcgggcg
    12841 ctgttccggc tctccgacga gctgatcgcg cgctgcgacg ccgccgacgt cgcctactcc
    12901 ggctcgatcg tcacgaacgg ctatctgctc gacgaggcga cctgcgagga gctcgcgagc
    12961 aggcggatcg acaacgcgca ggtcacgatc gacgggccgc cggacgtcca caacaagatg
    13021 cgacccctgg tcagcggggg aaagtcgttc gaccggatcg tggcgaacct gaaggccgcg
    13081 gtccgtcaca tgagcatcgc ggtcagggtc aacctggaca cctcgaacgt gcaccgggtc
    13141 gaggagctca tgaagatcct cgcggacgag ggcctgtcgg ggaagttggg cgtctacgcc
    13201 gctcagctca cgcaggtcga cgacggggca cccgcgccgt cggcgagcta cgccacggcg
    13261 tgcttctcgt gcggcgagtt ctccgaggtg cagtcggagt tccgcgagct ggccgagtcg
    13321 tacgggttcg acagtccgtc gcttccgggg ccgtccggcg cgccgtgcac ggcggtgcgc
    13381 gcgaacgagc tcgtgatcgg gagcaagggc gagctgtaca agtgctggga ctcggtcggg
    13441 aacccggcgg aggtcgtcgg gcacatccgc gactaccgca aggcggagac gcgcgcggcg
    13501 aagtggctgt cctacgaccc cttcgagaac gacgagtggc gctcgtgcat cgcgctgcca
    13561 gtctgcatgg gcgggtgcgc gcaccacggg atggacgaga agctctacga cgaccggtgc
    13621 atgccgttca ggtggaacta cgcggagcag gtgcggtcgt tcgtgaaggc gaacgccgac
    13681 gggacggccg agccggtcgt gctgagcgcc gcggggcata cccgctaggg gggcgaactt
    13741 cggcggcgcg gcccttaagg gggcatgctc cgcagatcag ccatcgccgt ggcggccgtg
    13801 ctcgccgccc tcgccgccgc cccgcccgcg ggcgcgagcc tcgtcgcgct cggtgactcg
    13861 ttctcgtccg gcgagggcgc ccctccgttc gagccgggcc actgccaccg ctccccgcac
    13921 gcgtggccgc tggtcctggc cgcgcggctc gacgcgccgg gcacgtcgag ggcgtgcagc
    13981 ggtgcggtgg tggcggacgt cgtccaggat cagatccccc gggtggcccc cggcgcggac
    14041 gtcctgacgc tcacgatcgg cggcaacgac ctgggcttcg ccgaggtgct gcggaggtgc
    14101 gtgctcgctg attgcgtgag gcactacgcg cgcgccggcc gcgacgcgat cgacgctcgg
    14161 gtacgggccc tgcgcgcgcg gctgcccgcg ctgtaccggc gggtccgctc ggccgccccc
    14221 ggcgccgggc tcgtcgtgct gggctatccg cggctcttcc cgcgccgccc cggccgcttc
    14281 acctgcgcgg cgctcggcgc gatctcgcgg gccgaggcgc tctacctgaa cgcgaagacc
    14341 gcggcggccg accgggcgat ccgggcggcg gcgcgcgacg ccggcgccct ctacgtggag
    14401 accctggacg cgttcgacgg cggcgaggtc aggtgcgcgc cccgccggcg ctacgtcaac
    14461 gacctcacgt tcctgcgcgg cggcgtgatc agcccctact cgtttcaccc gaccgccgcg
    14521 ggacacgcgc gcctcgccgc cctcgccctc gacggcctcg ccgcgaacgt gagggaccgc
    14581 cggtagcctg cctgaccatg gacttctcac cctcaccgcg cgtcgaggag ctcaagggcc
    14641 ggatcgacgc cttcctcgac gagcactact acccgcacga gctggacatc ttcaacgagc
    14701 tcgacgagga ggtcgggccc ggcgtcgcgt acccgaagcg cctggtcgag ctgcgcgagc
    14761 gcgcgaaggc ggagggactc tggaacctgt tcctgcccgg cgaggagcac ggccccggcc
    14821 tgaccaactg ggagtacggg atgatctgcg aggcgatggg gcgcagcccg atcgcgccga
    14881 tggtcttcaa ctgctcggcg cccgacaccg gcaacgccga gatcctcgtg gaccacggca
    14941 cgccggagca gaagcaggag tggctcgagc cgctgctcga gggcacgatc cgcagctgct
    15001 tctcgatgac cgagcccgag accgcgggct ccgatcccac cggcctgcgc ggcacggccg
    15061 tgctcgacgg cgacgagtgg gtgatcgacg cgcacaagtg gttcacgtcc ggcgcggtgg
    15121 gcgcctcggt ggcgatcgtg atggtcgtga ccgaccccga cgccgccccg cacaagcgcg
    15181 cgagcatgat cctcgtgccc accgacgcgc cgggcttcaa cctcgtccgg cccgtctcgg
    15241 tgatgggcca ttccggcggc ccgggtcact gcgagatccg ctacgagtct tgccgggtgc
    15301 cgaaggacaa cctcctcggc gagcgcgggg ccgggttcaa gatcgcccag gaccgccttg
    15361 ggccggggcg catccaccac tgcatgcgcg cgatcggcgc ggccgagcgc gctcacgaga
    15421 tgatgtgccg gcgcgcgacc gagcggcacg cgttcggcgg tccgctggcg gagaagcagt
    15481 tcgtccagga cttcatcgcg aagtcgcgga tcgagatcga cggcgcacga ctgatggtgc
    15541 tgaacgccgc gtggcagatg gacgagctcg gcaagcgcga ggcgcgccag gcgatctcga
    15601 tgaccaaggt cgtggccgcg aacgtggtga tggacgtgct cgaccgcgcg atccaggtcc
    15661 acggcgcgct cggggtttcc gacgacaccc cgctcgcggc gatgtggcgc gggctgcgga
    15721 tgctgcggct cgccgacggc ccggacgagg tccacaagat ggtgatcgcg ctgcgcgagc
    15781 tgaaccgcta ccgcgacaag gaaccgtccg gcggaggcaa cgggctcgcc ccggtgcagc
    15841 ccccggcggg cgtcggctcg ggggcgtcgt agccgtgctc gacgccacgc tcacgcgcaa
    15901 caacgacatc gccctcagcg acgagcagca ggacttcgtc cgctcgatcc gcgacttctg
    15961 ccagcgcgag ttccccaagg acaagctcga gcagctcacc gacggcttca aggacatcca
    16021 caacgacgac gtcgcgcgca agatggccga gctcggctgg ttcgggctca ccatcgacga
    16081 ggagtacggc ggctcgggcg gcacgttcct cgacgcggcg ctgttcctcg aggagagcgc
    16141 ccacggccgc gcgccggtga gcggctaccc ggtcacgctg atcgtggtgg gcgcgctcaa
    16201 ccgcttcggc accgaggagc agaagcgcga cctgctcggc cgcgtggccg cgggcggcgg
    16261 gctcgccatc gccatgtccg agccggagtc gggctccgac gtcgcgtcgc tgaagaccaa
    16321 ggcgcgtcgc gagaacggcg agtgggtcct gagcggttcg aagatgtggt gctcgtacgc
    16381 gcacaaggcg agccacacgc tgatcgtgtg ccgcaccggc gagggctcga gcaaccacga
    16441 gggcctatcg atgatcttcg tgccgcgcga cgccgagggt ttcacgatca cgccgattcc
    16501 gacgctcggc ggcgaggaga cgaacgagct ctacctcgac gacgtccgcg tgcccgagga
    16561 gaacctgctc ggcaccgagg gcaacggctg gacgcaactc atggccggcc tcaacaacga
    16621 gcgcgtgatc ctcggggccg gcgcgctcgg cctcgcgcag cgcgcgttcg acgacgcgct
    16681 ggcgtacgcg aaggagcgca agcagttcgg gcgcccgatc ggcggcttcc aggcgatcca
    16741 gcacaagttc gccgacatgg cgaccgggct cgcgcaggcg cggctgctcg tgcgctgggt
    16801 cgcgcggctg accgacgagg acgccgggcg gatgctcccg caggaggcgt cgagggtgaa
    16861 gctcgcgtgc acggagcttg cgaagaagtg cacgctcgaa ggcgtgcaga tcatgggcgg
    16921 ctacggctac gcgaccgagt atccgatgga gcgctacctg cgcgcggcgg tggtcaccac
    16981 catctacggc ggcacctccg agatccagaa gaacatcatc gcgaagacgc tcggccttta
    17041 gcgcgcgacg cgcttcgccc aacggtggcc ccgaggggct tctgggtcta ttcttccgag
    17101 cgttgaagtg actcgttcgg tgcgtcttga ccggcgcgcc cggaatgccg atctggattg
    17161 aacatggccg gattcgtcgg cgggagggct aggagactcg cagcgttggc tgtcagcgtg
    17221 acggccgttt cggcgtgctc gagcgcgtac gcgcaggttc ccgacctccc gcaggcgccg
    17281 cggctgcccg acacgccggt cacgcagccg gctcagaatg tcgccgacca agcgtccgac
    17341 acggcgaacc aggtgaccgg tcaggtcaac cagaccgtga acccgcaggg cggcggcagc
    17401 cagccgagtg ggggcggagg cggcggcacc ggaggcggcg gtggtggtgg caacatgccg
    17461 agcggcaacg gcggcggctc aagcggcggc ggatcgaaca gcggcgacgg cggctccgac
    17521 ggcggcggcg acaacggctc gtccagaggc ggctcgacgg ccgccgagcg ccgggccgac
    17581 cggcgtgccc gcacgcgggc cgcggcacgg cgcgccgagc gccggcgcga gcgcaccgcg
    17641 gcgcgtggcg acgagggcga ggaccgcccg tcccgcgacg gcgacaacgg ccccggcccg
    17701 ctgcggccga tccgcgacgt ggtcgaggtg atccccgtcc cgatgtggat cgcgctgggg
    17761 ctactcgccc tgctcgcggc gctgttctac gtgcgcggct tcctcacggc ccggcgagcg
    17821 cgtcagctcg aggcgcagcg ctcggagctg ctcgacgacg tcggcctgct ccagaaggcc
    17881 ctgctgcccg acgtgcccga gcggatcggg ggtctcgagg cgtcggttgc ctaccgcccg
    17941 gccgacggcc cggcggccgg aggcgacttc tacgacgtgt tcgagatgga cgccggccgc
    18001 gtcgggatca tcgtcggcga cgtgtgcggc cacggccgcc aggcgctcgc cgtcaccgcg
    18061 ctgatgcgct acacgctgcg cgcctacctg aacgccggcg gcgagccgcg cgcggcgctc
    18121 cagatcgcgg cgcgggcgct cgagaacgag cagcacgccg agctgaccac cgtcgtcctc
    18181 gccgtttacg acgcaaaggc cggcaccctc acctacgcgt gcgcggggca cgagccgccg
    18241 atcctcgtcg gcccggccgc gcacaagccg gtcaccgtgg gctcctcccc gccgatcggc
    18301 gccggtctcg tgaccggcct gcgccagacc accgtcgcgc tcccggccgg ctcgctggcc
    18361 tgcttcttca cggacgggct cgtcgaggcc cgcctcgacg acgacgccct gctcggccgc
    18421 gaccggctct gcacgatcgt cgagcagctt ggcgacgggg catgtgcgga cgcgctgctc
    18481 gccaagatcg ccagcgatgc cgagcgggcg ccggacgaca tggccgcgtg catcatccgc
    18541 gcggccgccg acgctccggt cgtctccgac gtccgcatcg aggagttcga gctcgacgac
    18601 aacggggtcg gggaggagcg cgcccgcggc ttcctcgcgg cctgcggcgt tccggagagc
    18661 gaggccgaca ccgtgatcaa gaccgcgcgc ggcgcccgcg ccgagttcgg cggggcgatg
    18721 ctgcgcgtgc gcctgagcgg cagcggcggc ggcgtcaagg tcgtcccgct cgagctgcgc
    18781 ccgctggcgc cctccacgaa cgggcaccac ccgccggccg tcgtgccggt cgcgccgccg
    18841 atctcggcgt aaccccgctt acgcagcgct ccttgcggtt attgccgtat tccgctccct
    18901 gggttacgtt gaaaagccca acaggaggct caaacttgcg gaagctaccg atcgctgtcg
    18961 tactcgccgt cgtcgcctcg ctggcgttct ccagcgtcgc ctcggcgcac accctgaggt
    19021 acaacacggc caagaacctg gccgtgaagc ttgcaaagaa gcaggagagg aacgatcccg
    19081 tcaagcgctg gcggatcttc ggggccgagc ggcacagcga ccacgagatc tcctggacct
    19141 acaacgtgga ctacgacgac ggctcgctat gcgacgccga gctcgtcgtg cgctacacga
    19201 ccaagtcgaa cggcaagccg atcgtgcgcg cgttcttccg ccacgtggag tgcactcgct
    19261 aggaggcgcg cagctcgtcg agccgccgcc gccagtccgc ctcgacctct gaggccggac
    19321 gcccgaaggc ccgctggatc gccgccgcgt cccccgcttc aggggggcgg gcggcgagcc
    19381 gcacgcactc ctccgggccc agctcgtcct cgagcatcgc gaacacggtg ccgcccagca
    19441 gcacggcgtc gcgcgcctgc ggcgggaagg cgggccgccc gccctcccgc agacgccgcg
    19501 cgatggccgg ccgcaggtgc ttggtctgcc ccgcgaggtg ggtggcggct ccctcgcaca
    19561 gccacgccca gcgcaggtag cgggcgaacg tgcgcggcgt gaacggcggc ggcagcgtgg
    19621 tgttgttcgc gccgatcacg acgtgggcgt actcgtggag cgggctgagc agcagcgcct
    19681 cgcgcgagcc ggggaccgcg gaggcccgct cttcgagcgc ggcgggcgct agcacgtgga
    19741 tctcgcggcg gccgaaccag cccgccatgt agcggcgcgc cgcgggggcc gccgccatgc
    19801 gggccaccgg gagccagggg tgagcgagcg aaagcgcgag cggtccgggg tgcatgatca
    19861 cggccacctc gccgggggtg ctgccgaagg cgtcgtcgag ctcgtcgcgg aagcgctcga
    19921 gctgctcgag catggcggcg gcggcatcgg tgtcgcgctc ctcgtggcgc gcggaaaacg
    19981 acggcgagtg cgactcgatc cagggcacgc cgcctactct cgcagcgggc gcgccgcgcg
    20041 ggcgcccgcc cccggcgcta ccgcttcggc ccgtgccgcg tggtcttcgg cttcgactgc
    20101 ttgcagccct tgtagacccg cgtgctgatg atctcggtgc cgttcgagtg gacggcgcgg
    20161 atgcggacca cgaagcgctt ctgcggcagc ttctcgagcg cgatccggcg cacgtccttg
    20221 cccttcacgc gcagcctgcg cttgccgttc acgt
//
LOCUS       DASVPL010000084         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_284752, whole genome shotgun sequence.
ACCESSION   DASVPL010000084 DASVPL010000000
VERSION     DASVPL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284752"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF517_03875"
     CDS             complement(<1..317)
                     /locus_tag="VF517_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052106.1"
                     /translation="MRRLLATFAVLAAALAVPATAQTPGDPVRFDPSVAGKASHLIVD
                     VRSAEDPQANGRSPQSVVMAVTQGFKFDPRARSERCSDAQAKAFDCPGNSKIGSGTAN
                     ITA"
     gene            complement(416..586)
                     /locus_tag="VF517_03880"
     CDS             complement(416..586)
                     /locus_tag="VF517_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052107.1"
                     /translation="MVDVALSLAELDASWGDYNHALRFLDSADELTSGAIRPRWAARR
                     RRWVDAAQGFSP"
     gene            789..1298
                     /locus_tag="VF517_03885"
     CDS             789..1298
                     /locus_tag="VF517_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX8052108.1"
                     /translation="MDLEAAIRTRRTHKAYGPDPVAREVLDALFELARWAPNHNLTNP
                     WRFRVVGPESLERLKAAAGPEGATKLDRAPTLVVATALQCDDAVQDEEDFAAAACAVY
                     VVLLAAHARGLAGYWRTPAVLRTPEGRAAVGIPDGERVLGLIHLGPPRQGRDAPERAP
                     VDEYVTYLA"
     gene            1295..>2086
                     /locus_tag="VF517_03890"
     CDS             1295..>2086
                     /locus_tag="VF517_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8052109.1"
                     /translation="MIAREDALAAFEADRFDMVVIGGGITGAGVALDAASRGYSVALV
                     ERDDFAAGTSSRSSKLVHGGLRYLQNFDLGLVREALLERALMVNLAPHLVHPLALLIP
                     TFEGKHLDRPTGVGLNAYDVMATERIRLPSLRRRRDAPSPGLSLEEWSPERHRVVDAD
                     EVVRLIPALAGRNPDSAYLFYDCQTDDVRLVLTVLGEAERFGAVMANRSRVLELLEEN
                     GRADGVRCRDELGGGEFELRADTVVNATGVWADTIRSEEVRDAAEV"
BASE COUNT          275 a          736 c          785 g          290 t
ORIGIN      
        1 ctggccgtga tgttcgccgt cccggagccg atcttgctgt tgcccgggca gtcgaacgcc
       61 ttggcctgcg cgtccgagca gcgctccgac cgcgcgcggg ggtcgaactt gaagccctgg
      121 gtgacggcca tgacgacgga ctggggcgag cggccgttcg cctgcgggtc ctcggcgctg
      181 cgcacgtcca cgatcaggtg cgacgccttg cctgccacgg acgggtcgaa gcgcacggga
      241 tcgccgggcg tctgcgcggt cgcgggaacc gccagcgccg cggcgaggac tgcgaaggtt
      301 gcgaggaggc ggcgcacggg tcgaagcatt tctagcagcc gcccggaagt tgcgcgccgc
      361 cggccggcga ggctcatccc cggccggcgg acgcagttct cccccttcga gggggtcagg
      421 gggagaaacc ttgtgccgcg tctacccagc ggcggcgccg ggccgcccag cgcgggcgga
      481 tcgccccgct cgtgagctcg tcggcggaat cgaggaagcg aagcgcgtgg ttgtagtcgc
      541 cccagctcgc gtcgagctcg gcgagcgaga gggcgacgtc gaccatggcg cggcgctcgt
      601 agtcgtggcg acgctcatgc gtgggggctt ttagctggga cacgggacct tggagggagg
      661 tcgtgcgggg gacgacggag tctcctccgg cggcaccgga tgacaaggac gttgcagaat
      721 cgatgtgagc tatttgcggg aagcgccgca ctcgccgcag gattcgcgcc ttcgctagct
      781 tcggccccat ggacctcgag gccgcgatac gaacgcgccg cacgcacaag gcctacggtc
      841 cggacccggt cgcgcgcgag gttctcgacg cgctgttcga gctggcccgc tgggccccca
      901 accacaacct cacgaacccg tggcgcttca gggtcgtggg ccccgagtcg ctcgaacgcc
      961 tgaaggccgc ggctggcccc gagggcgcca ccaagctcga ccgcgcgccg acgctcgtgg
     1021 tcgccaccgc gctccagtgc gacgacgcgg tgcaggacga ggaggacttc gccgcggccg
     1081 cctgcgcggt gtacgtggtg ctgctcgccg cgcacgcgcg cgggctcgcc ggctactggc
     1141 gtacgccggc ggtgctgcgc acgccggagg gtcgcgcggc ggtggggatc ccggacggcg
     1201 agcgcgtgct cggcctgatc cacctgggcc cgccgcgcca gggccgcgac gcacccgagc
     1261 gcgcgccggt ggacgagtac gtaacgtacc tggcgtgatc gcgcgcgagg acgcactggc
     1321 cgcgttcgag gccgaccgct tcgacatggt cgtgatcggc ggcgggatca ccggcgcggg
     1381 cgttgccctc gacgccgcct cgcgcggcta cagcgtggcc ctggtcgagc gcgacgactt
     1441 cgcggcgggc acctcgagcc gctcgtcgaa gctcgtgcac ggcggtcttc gctacctcca
     1501 gaacttcgac ctcgggctgg tccgcgaggc gctgctcgag cgcgcgctga tggtcaacct
     1561 cgcgccgcac ctcgtccacc cgctggctct cctgatcccg accttcgagg gcaagcacct
     1621 cgaccgcccg accggcgtgg gcctcaacgc ctacgacgtg atggcgaccg agcgcatccg
     1681 gctgccgagc ctgcgccgcc gccgcgacgc gccctcgccg gggctctcgc tcgaggagtg
     1741 gagcccggag cgccaccgcg tggtcgacgc cgacgaggtg gtgcgcctga tccccgcgct
     1801 cgccggtcgc aatccggact cggcctacct cttctacgac tgccagaccg acgacgtgcg
     1861 gctggtgctc acggtgctcg gggaggcgga gcgtttcggc gcggtgatgg cgaaccgcag
     1921 ccgcgtgctc gagctgctcg aggagaacgg ccgcgccgac ggagtgcgct gccgcgacga
     1981 gctcggcggg ggcgagttcg agctgcgtgc cgacaccgtg gtgaacgcga ccggcgtctg
     2041 ggctgacacg atccgctcgg aggaggtgcg cgacgccgcg gaggtc
//
LOCUS       DASVPL010000085        13053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_246474, whole genome shotgun sequence.
ACCESSION   DASVPL010000085 DASVPL010000000
VERSION     DASVPL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246474"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..5950
                     /locus_tag="VF517_03895"
     CDS             11..5950
                     /locus_tag="VF517_03895"
                     /inference="COORDINATES: protein motif:HMM:NF028019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052110.1"
                     /translation="MVVSATNAAATVAATSNQTSTVAAAPPVNTGLPSITGTPQDGQT
                     LSASNGSWSGTAPFTYADQWRRCDASGASCVNIAGATGSTYVVQAADVTSPASTLRAV
                     LTATNSAGNAAATSAQTASVTPAPPVNTALPAISGTPEHGATLSASTGTWTGSTPIAY
                     TYQWRRCDSSGATCSNISGATSSSYTVQSADLTSPASTIRVVVTATNAAGNAAATSAQ
                     TASVTPAPPVNTALPTISGTPQHGQTLTAAPGTWTGTPTISYIYQWRRCDSTGAACVD
                     ISSATGSTYVVQQADVTTPASTIRVRVTASNAGGFGSANSSQTTAVAAAAPANSSPPS
                     ISGTTQDGQTLSSTNGSWSGTAPFTYAYQWRRCDSAGVNCANIAGATGATYALAPADI
                     GSTLRSVVTATGPGGTQSSTSSQTAVVTAIPPTNTAPPTISGSPQEGSTLTASNGTWT
                     GSPTIAYAYQWRRCDSAGASCSSISGATASTYVVQQADVTSPATTIRVAVTATNAGGS
                     QSATSAQTGAVGGFSPVNTGAPAISGTTRDGQTLSATNGSWTGTAPFSYAYQWQRCNA
                     AGASCSNITGATATTYDLTPADVGATIRVVVTATNPAGDGNATSSQTAVVTARPPVNT
                     SLPTISGTTEDGRTLSATTGAWTGTPTITYAYQWHRCDSAGAGCSDISGATSASYALT
                     PADVGATIRVRVTASNAGGDDSASSTQTAAVAPTAPVNTVAPVVTGTTEDGATLTAST
                     GIWSGTPTIGFGYQWQRCDTAGASCSDISGATATTYGLTPADVGATIRVVVTGTNAAG
                     SDDAASAATDTVGVDEPANSGAPTISGTPEDGETLTAHPGSWTGTPTIGFTYQWQRCD
                     AAGAGCVDVGGATGSTYDLGPADVGARMRVVVTGTNAGGGASSPSAATAEVAPDAPVN
                     TSLPTVSGTSEDGHVLSAGNGGWTGTPTIDFDYQWRRCDSAGASCSDISGATGSTYEL
                     TPADVGATLRVVVTATNAGGSDSATSAKSSTIAATAPVNTAPPVVSGTLEHGSTLSST
                     TGAWTGTPTIGFGYQWRRCDSAGASCSNISGATGSTYQLTAADIGATIRVVVTGTNGA
                     GSDSAASAASAKVGAAAPTAASPAPSILGLTRDGEILSATTGGWNGTAPSSYGYQWRR
                     CDATGAACQNVSGATGSIYTLVPADVGSTMRVVVTATNAAGSASATSDKSAVVTVVAP
                     ANTAAPVVTGTPTDGSTLSSTTGAWTGTPTIAFARQWRRCDAAGASCADIAGATGATY
                     TLTSADIGATIRVRVTATNAGGSASASSTASATVAHRGPSSTSAPTISGTPRHGQTLT
                     ATPGGWDGSAPIAHGYQWRRCNLAGTSCTDIPGATSSTYVLGAGDVGSTIRVVVTGTN
                     PGGSASATSGQTAAVAGNPPVNSAAPVISGTLEDGSTLSSTTGSWTGTPTIAFARQWR
                     RCDAAGAACVDIAGATGTTYGLTAADLGKTIRVVVTATNMAGSAGATAAQTGAIDPAK
                     PTSTGAPTISGTPKQGETLTATPGTWTGTAPLSHAYQWQRCAADGSSCVDIPGAGDPT
                     YVLAGDDVDKTIRVKVTTTNGGGSTSSGSSQTARVEPLPVVEPKPPVEEPKPPVVEPK
                     PPVNDGIPTISGEPKSGTPLTADPGHWAPAADRFDYQWERCDAAGANCTTIPGATAIT
                     YTPTDADDGARIRVSVTGINGDGHRSASSVPTAAVQVDLGAIDESLVGPGKCTKITAG
                     TGVKRKVVKGLGTVKILLRASAFVAPADPLRLSTTASSGTLKAVRYTLDGRNVGQPKR
                     KPYWQNIMPSALSVSGGDAHKVGVTLVPARGPAVTFAFDVKTKPCDNMLSTTQWKTAK
                     GAGLRLRVDSRGQLGPVQFRVPAAMLPKARDVNKGVGRVRVFMKSGPPKPYTLAMTKE
                     KKMVLLDGEGKPRVEIVRGGAVVSNLPAGTGIVELTLYTQKATSPRALLAKGKRATLG
                     ATTTSAGAPVTLSSVLVGRGR"
     gene            6038..9511
                     /gene="dnaE"
                     /locus_tag="VF517_03900"
     CDS             6038..9511
                     /gene="dnaE"
                     /locus_tag="VF517_03900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX8052111.1"
                     /translation="MPPAVHLHVHSEYSLLDGACKIDGLAARAAELGQPALGLTDHGV
                     MNGAVEFQKACQKHGIKPILGFEAYLCDDLTSDAVRYERNHLTLLARTDEGLRNLVKL
                     TSAGFLEGNKRGKCNVDMALLERHSAGVIALTGCLQSRFCRRLVDDLPGEARAHADDL
                     ARVFGEDNVYFEVQKNGIADQDKANEGIVRIAREMGRPLVATADVHYLRREDYSNHSA
                     LLCVQTKSTLAAPKLSFDTNEFFLKSNEEMADSFVEWPEAIATTTEIAERCDASIELG
                     KMLIPSYPVPDGRPEPEYLRELAMDGLRRRYGDPIPAAAVERLDMELEVIVKMGFAAY
                     FLIVWDFVKFAKDNGIAVGPGRGSAAGSIVSYALSITDVDPLRYGLLFERFLNAERVS
                     MPDIDIDFSVKGRERVIRYVAAKYGQDSVAQIVTFGRMFPRAATRDAARVLGFDYGAG
                     DRLAKLIPDPIMGRPPSFEDCLKPGEELARAYKEDAQAKQIVDVARGLEGVVRNSSIH
                     AAAVVIADRPLTDIVPLQLAEDRGEVNEDGGRAYRTVTQYSMKPIEEIGLLKMDFLGL
                     RNLDVIEAALDIVERSSGERPDMAKLPLDDAKTYEMLARGDSIGVFQFESEGMREALK
                     KVRPTEFEDLVALVALYRPGAMRHIDTYARNKRNPDAINYIDDRLRPITDTTYSVILY
                     QEQSMQIAKEIAGFTGPQADDLRKAIGKKDRAKMASMKDLFVQGARATGTADRVIQDL
                     WETNEAAADYSFNKSHAACYALIAYRTAWLKANYPAEYMAALISSVMSTKDKVPFFVS
                     RCEEMGIEVLPPDVNESGHDFVVTAGNIRFGLDAVKNVGAAAVDAVMTAREEDGPFTS
                     IWDFCERVDCQRVNKKAIESLVRCGAFDSLGASRRGMLEILPQAQAAGQKVQQDAQMG
                     QASIFDLDEGPAAMQAPRQHPPVPLIEDERAELNAMEKETLGLFLSSHPFKEVRAALR
                     LKVDCGLADLAKKRDGEWVTVGGMIAEAKKIRTKKGDPMLFATLDDLEAQVEILVFSK
                     AYSANAEKIDTDNVVIVRGRVDHKEQGETKVVVQECEPFEPTPDEVERAREQAAVILA
                     PPRLKLRVSPGIDAGWLEDLKELVKHNKGDHELVVCVGDRTLVLGEQYRVSPGRFCAE
                     LQELPGAPELV"
     gene            complement(9537..9920)
                     /locus_tag="VF517_03905"
     CDS             complement(9537..9920)
                     /locus_tag="VF517_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8052112.1"
                     /translation="MGQPVMHFEVIGKDGQKLRSYYSELFGWELHEVGGPMDYATVDR
                     EQNLSPEGIGIGGGIGGSSEADGHVTFYVGVDDAEAYLAKAEELGGTRVWGPDEVPGA
                     GIVLGHLKDPEGHLVGVVQPTGDQQ"
     gene            10117..10503
                     /locus_tag="VF517_03910"
     CDS             10117..10503
                     /locus_tag="VF517_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052113.1"
                     /translation="MAQTAHPGQLSECRQCCSFCDRVLLPSGCLDSNCQYLYLYDDED
                     NGRRYMGCLNKVFKVEIDLELFRESERTRHGFGGVKLTGTPIPRCRTAVERAYHGGGD
                     AFDCVNPMFFDDPAPPAADFDLRDAL"
     gene            complement(10617..11852)
                     /locus_tag="VF517_03915"
     CDS             complement(10617..11852)
                     /locus_tag="VF517_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX8052114.1"
                     /translation="MLIGGHVSPAGGLVNAFHRGVERDCDAIQIFNQSPRMWRPTNYR
                     DDDIAEFRELLAAGPVTSIVIHAVYLINCASKDPVIREKSLASLSHALRVGDAIGASG
                     VVVHPGSTVGEPLGESLRRVGEAVRHVLEESENCMLLLENTAGAGGTIGRSFGELAEL
                     LELGGGGPRLGICLDSCHMLASGFDVRTAEGLSEVLDECVASLDLDRLRCLHVNDSMM
                     PLGSNRDRHAPLGDGELGPEGCAAFFSEPRFEGLPAIFEGPGLAGKGAAYEDMAVARR
                     LRNEGLAARGLPVVERTPHGKPEAKAKPKAKAKVAKGKAGAKAKPKAAAKPKAAAKPK
                     AAAKPRGSARTAAPKGGGSKAKPKAGAKPKAGSAKPKAAAAPKAGSAKPKAAAKPKPK
                     AAAAPKAGGSAKRSSKAST"
     gene            11871..>13053
                     /gene="gltX"
                     /locus_tag="VF517_03920"
     CDS             11871..>13053
                     /gene="gltX"
                     /locus_tag="VF517_03920"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX8052115.1"
                     /translation="MRLRFAPSPTGALHIGGARTALYNWLLARQSGGAFVLRIEDTDR
                     ERSTPENVAQIHDALDWLELDVDEGPHSQSERRPRHEEEIARLLGEDHAYYDEGAVRL
                     RVPDGGQTIVNDVIRGEIVFDHEAIDDFVIARSDGSPLYNLAVAVDDRDMGITHVVRG
                     ADHLSNTPRQLMILKALGAEPPVYAHVPLLHGTDGKKLSKRHGAASVQELRAAGYLPE
                     AVRNYVALLGWGYDESTTFFTTEELIEHFSLERVSKSPAVFDEQKLRWMNGRYQREMT
                     LDELHARLERFHDRPLPREAVAISQEKMQTLAEFWPLAGFLVEPQPTDEKAWKKVMGD
                     GAGERLARAREAIASVEPFEPEALESRLAPLVDELGVKARDLYQPIRVAISGSTISPG
                     IF"
BASE COUNT         2042 a         4992 c         4390 g         1629 t
ORIGIN      
        1 gacgatccgc gtggtcgtct ccgccacgaa cgccgccgcg acggtcgcgg ccacgagcaa
       61 ccagacctcg acggtcgccg ccgccccgcc ggtcaacacg ggcctgccct cgatcaccgg
      121 caccccgcag gacggccaga cgctcagcgc cagcaacggc agctggagcg gcaccgcgcc
      181 gttcacctac gccgaccagt ggcgccgctg cgacgcctcc ggcgcgagct gcgtgaacat
      241 cgcgggtgcc acgggctcga cctacgtggt gcaggccgcc gacgtgacct cgcccgcgtc
      301 gacgctccgc gccgtgttga ccgcgaccaa cagcgcgggc aacgccgccg cgacgagcgc
      361 ccagacggcg agcgtcaccc cggccccgcc ggtgaacacg gcgctgccgg cgatcagcgg
      421 caccccggag cacggcgcga ccctgagcgc ttcgaccggc acctggaccg gcagcacgcc
      481 gatcgcctac acctaccagt ggcgccgctg cgactcctcg ggcgcgacct gctcgaacat
      541 ctccggtgcc acgagctcct cctacacggt ccagtccgcc gacctcacct cgccggcatc
      601 gacgatccgc gtggtcgtga ccgccaccaa cgccgccggc aacgccgcgg ccaccagcgc
      661 gcagaccgcg agcgtgaccc cggccccgcc ggtgaacacc gcgctgccca cgatctccgg
      721 cacgccgcag cacggccaga ccctcaccgc cgcgcccggc acctggaccg ggacgccgac
      781 gatcagctac atctaccagt ggcgccgttg cgactccacc ggcgccgcgt gcgtcgacat
      841 ctcgagcgcg acgggctcca cctacgtggt gcagcaggcc gacgtgacca cgccggcatc
      901 caccatccgg gttcgcgtta cggcgtccaa cgcgggcggc ttcggctcgg ccaacagctc
      961 ccagaccacg gcggtcgccg cggccgcgcc ggccaacagc tccccgccgt cgatctccgg
     1021 cacgacccag gacggccaga cgctcagctc gaccaacggc agctggagcg gcaccgcgcc
     1081 gttcacctac gcctaccagt ggcgccgctg cgactcggcc ggcgtgaact gcgcgaacat
     1141 cgcgggcgcg acgggcgcta cctacgcgct ggccccggcc gacatcggct cgacgctgcg
     1201 ctctgtcgtc accgcgaccg gccccggcgg cacccagagc tcgaccagca gccagaccgc
     1261 cgtcgtcacc gcgatcccgc cgaccaacac ggcgccgccg acgatctcgg gcagcccgca
     1321 ggagggctcg acgctgaccg cgtcgaacgg gacctggacc ggatcgccga cgatcgccta
     1381 tgcctaccag tggcgccgct gcgacagcgc gggcgcgagc tgctcgagca tctccggcgc
     1441 gaccgcctcg acctacgtgg tgcagcaggc ggacgtgacc tcgcccgcga cgaccatccg
     1501 cgtggccgtg accgccacca acgccggcgg cagccagagc gccaccagcg cgcagacggg
     1561 cgccgtcggc ggcttcagcc cggtcaacac cggcgcgccg gcgatctccg gcaccacgcg
     1621 cgacggccag accctgagcg cgaccaacgg gagctggacc ggcacggcgc cgttcagcta
     1681 cgcgtaccag tggcagcgct gcaacgccgc gggcgcgagc tgctcgaaca tcaccggcgc
     1741 cacggcgacg acctacgacc tgaccccggc cgacgtgggt gcgacgatcc gcgtggtcgt
     1801 gaccgccacg aacccggccg gcgacggcaa cgcgaccagc tcgcagaccg cggtcgtgac
     1861 cgcgcgcccg ccggtcaaca cctcgctccc gaccatctcc ggcacgaccg aggacggccg
     1921 cacgctgagc gcgacgaccg gcgcatggac gggcaccccg acgatcacct acgcctacca
     1981 gtggcaccgc tgcgactccg ccggggcggg ctgctcggac atctccggcg cgacctccgc
     2041 gagctacgcg ctgaccccgg ccgacgtggg cgccacgatc cgcgtgcggg tcacggcatc
     2101 gaacgcgggc ggcgacgaca gcgccagcag cacccagacc gcggccgtcg ccccgaccgc
     2161 ccccgtcaac acggtcgccc ccgtcgtcac gggcacgacc gaggacggcg ccacgctcac
     2221 cgcgagcacc ggcatctgga gcggcacccc gacgatcggc ttcggctacc agtggcagcg
     2281 ctgcgacacc gccggcgcga gctgctctga catctccggc gcgacggcga cgacctatgg
     2341 cctgaccccg gccgacgtgg gcgcgacgat ccgcgtggtc gtgaccggca cgaacgccgc
     2401 cggcagcgac gacgctgcca gcgcggccac ggacacggtc ggcgtggacg agccggccaa
     2461 cagcggcgcg ccgacgatct cgggcacccc ggaggacggc gagaccctca cggcccaccc
     2521 ggggagctgg accggcaccc cgaccatcgg cttcacctac cagtggcagc gctgcgacgc
     2581 ggccggcgcg ggctgcgtgg acgtgggcgg cgccaccggc tcgacctacg acctcggccc
     2641 ggccgacgtg ggcgcgcgga tgcgcgtcgt cgtgaccggg accaacgccg gcggcggcgc
     2701 cagctccccg agcgccgcca ctgcggaggt cgcccccgac gcgcccgtga acacctcgct
     2761 gccgaccgtc tccggcacga gcgaggacgg gcacgtgctc agcgccggca acggcggctg
     2821 gaccggcacc ccgaccatcg acttcgacta ccagtggcgc cgctgcgaca gcgccggcgc
     2881 gagctgctcg gacatctccg gcgcgaccgg ctccacctac gagctcaccc ccgccgacgt
     2941 gggcgcgacc ctccgcgtgg tggtcaccgc cacgaacgcc ggcggcagcg acagcgccac
     3001 gagcgcgaag agctccacga tcgccgccac ggcacccgtc aacacggccc cgcccgtggt
     3061 gagcggcacg ctcgagcacg ggtccacgct cagctcgacc accggtgcct ggaccggcac
     3121 cccgaccatc ggcttcggct accagtggcg ccgctgcgac tccgccggcg cgagctgctc
     3181 gaacatctcc ggcgcgaccg gctccaccta ccagctgacc gccgccgaca tcggcgcgac
     3241 gatcagggtg gtcgtgaccg gcacgaatgg cgccggcagc gacagcgccg ccagcgcggc
     3301 cagcgccaag gtcggcgccg ccgctccgac ggccgcctcg cccgccccgt cgatcctcgg
     3361 cctgacccgc gacggcgaga tcctcagcgc caccaccggc ggctggaacg gcacggcccc
     3421 gagctcgtac ggctaccagt ggcgccgctg cgacgccacg ggcgccgcct gccagaacgt
     3481 gagcggcgcc accggctcga tctacacgct ggtcccggcc gacgtcggct cgaccatgcg
     3541 cgtcgtcgtg accgcgacga acgcggccgg cagcgcgagc gcgaccagcg acaagtccgc
     3601 cgtcgtgacc gtcgtcgcgc cggcgaacac cgcggccccg gtcgtgaccg gcacgcccac
     3661 ggatggctcc acgctcagct cgaccaccgg tgcctggacc ggcaccccga cgatcgcctt
     3721 cgcgcgccag tggcgccgct gcgacgccgc gggcgcgagc tgcgcggaca tcgccggtgc
     3781 cacgggcgcg acctacacgc tgacctccgc cgacatcggc gcgacgattc gcgtgagggt
     3841 gaccgccacg aacgccggcg gctccgcctc agcgagcagc accgcgagcg ccacggtcgc
     3901 ccacaggggc ccgtcgagca cctcggcccc cacgatctcc ggcacgccac gccacggcca
     3961 gacgctcacc gccacgcccg gcggctggga cggctcggcc ccgatcgccc acggctacca
     4021 gtggcggcgc tgcaacctcg ccggaacgag ctgcacggac atccccggcg ccacgagctc
     4081 cacctacgtg ctcggcgcgg gcgacgtcgg ctcgacgatc cgcgtggtcg tgaccggcac
     4141 gaaccccggc ggtagcgcca gcgcgacgag cggccagacc gccgccgtgg ccggcaaccc
     4201 gccggtcaac agcgcggcgc cggtgatctc gggcacgctc gaggacggct ccacgctcag
     4261 ctcgaccacc ggcagctgga ccggcacgcc gacgatcgcc ttcgcgcgcc agtggcgccg
     4321 ctgcgacgcc gcgggcgccg cctgcgtcga catcgcgggc gccacgggca ccacctacgg
     4381 gctcacggcc gcggacctcg gcaagaccat ccgcgtggtc gtgaccgcca cgaacatggc
     4441 cggcagcgcc ggcgcgaccg ccgcccagac cggcgccatc gaccccgcga agccgacgag
     4501 caccggcgcc cccacgatct ccggcacgcc caagcagggc gagaccctca ccgcgacgcc
     4561 cggcacctgg accggcaccg cgccgctcag ccacgcctac cagtggcagc gctgcgccgc
     4621 cgacggcagc tcctgcgtcg acatccccgg cgccggcgac ccgacctacg tgctcgcggg
     4681 cgacgacgtc gacaagacca tccgcgtgaa ggtgacgacc accaacggcg gcggctccac
     4741 gagctccggc tcgtcgcaga ccgccagggt cgagccgctc ccggtcgtcg agccgaagcc
     4801 gccggtcgag gagcccaagc cgccggtcgt cgagccgaag cccccggtca acgacggcat
     4861 ccccacgatc tcaggcgagc cgaagtccgg caccccgctg acggccgacc cggggcactg
     4921 ggccccggcg gcggaccggt tcgactacca gtgggagcgc tgcgacgccg ctggcgcgaa
     4981 ctgcacgacg atccccggcg ccaccgccat cacctacacc ccgaccgacg ccgacgacgg
     5041 cgcgcgcatc cgcgtttcgg tgaccggcat caacggcgac ggccatcgct ccgccagctc
     5101 ggtgcccacc gcagccgtcc aggtggacct cggcgcgatc gacgagagcc tcgtcggccc
     5161 cggcaagtgc accaagatca cggccggcac cggcgtcaag cgcaaggtgg tcaagggcct
     5221 aggcaccgtc aagatcctgc tgcgcgcgag cgccttcgtg gcgcccgccg acccgctgcg
     5281 gctctcgacg accgcctcca gcggcacgct caaggccgtc cgctacaccc tcgacgggcg
     5341 caacgtcggc cagcccaagc gcaagcccta ctggcagaac atcatgccct cggccctcag
     5401 cgtgagcggc ggcgacgccc acaaggtcgg cgtgacgctc gtgcccgcca ggggcccggc
     5461 cgtgaccttc gcgttcgacg tgaagaccaa gccctgcgac aacatgctca gcacgaccca
     5521 gtggaagacc gccaagggcg ccggcctgcg cctgcgcgtg gacagccgcg gccagctcgg
     5581 cccggtccag ttcagggtcc cggcggcgat gctgccgaag gcccgcgacg tcaacaaggg
     5641 tgtcgggcgc gtccgcgtct tcatgaagag cggcccgccg aagccctaca cgctggcgat
     5701 gacgaaggag aagaagatgg tcctgctcga cggcgagggc aagccgcggg tcgagatcgt
     5761 gcgcggcggg gcggtcgtct cgaacctgcc cgcgggcacc ggcatcgtcg agctgaccct
     5821 ctacacgcag aaggcgacca gcccgcgggc gctcctcgcc aagggcaaga gggccaccct
     5881 gggggcaacg acgacctccg ccggggcgcc cgtcacgctc tcctcggtgc tcgtcggccg
     5941 cggccgctag gcccggcgcg ccgggctccc cgcagaaccc gcaaactgcg gctccggccc
     6001 gggcgttccg gccggaccca cgcttaatgt ggagcccgtg ccgcccgcgg ttcatctcca
     6061 cgtgcactcc gagtactcgc tgctcgacgg tgcctgcaag atcgacggcc tggccgcccg
     6121 cgccgcggag ctcggccagc ccgccctcgg cctgaccgac cacggcgtca tgaacggcgc
     6181 cgtcgagttc cagaaggcct gccagaagca cgggatcaag ccgatcctcg gcttcgaggc
     6241 ctacctctgc gacgacctga cctccgacgc cgtccgctac gagcgcaacc acctcaccct
     6301 gctggcgcgc accgacgagg ggctgcgcaa cctcgtcaag ctcacctccg ccggcttcct
     6361 cgagggcaac aagcgtggca agtgcaacgt cgacatggcg ctgctggagc gccattcggc
     6421 cggcgtgatc gccctgacgg gctgcctcca gtcgcgcttc tgccgcaggc tcgtggacga
     6481 cctgcccggc gaggcgcgcg cacacgcgga cgacctggcg cgcgtattcg gcgaggacaa
     6541 cgtctacttc gaggtccaga agaacgggat cgccgaccag gacaaggcca acgaggggat
     6601 cgtccggatc gcgcgcgaga tgggccggcc gctggtggcc acggccgacg tccactacct
     6661 gcgccgcgag gactactcga accacagcgc gctgctgtgc gtgcagacga agtcgacgct
     6721 ggcggcgccg aagctgtcgt tcgacaccaa cgagttcttc ctcaagtcga acgaggagat
     6781 ggcggattcg ttcgtcgagt ggccggaggc gatcgcgacc accaccgaga tcgccgagcg
     6841 ctgcgatgca tcgatcgagc tcggcaagat gctgattccg agctaccccg tgccggacgg
     6901 acggccggag cccgagtacc tgcgcgagct ggccatggac gggctgcggc gccgctacgg
     6961 cgacccgatc ccggcggccg cggtcgagcg cctcgacatg gagctcgagg tgatcgtgaa
     7021 gatgggcttc gcggcctact tcctgatcgt gtgggacttc gtgaagttcg cgaaggacaa
     7081 cgggatcgcg gtggggccgg ggcgtggctc ggccgcgggc tcgattgtct cctacgcgct
     7141 gagcatcacc gacgttgacc ccctgcgcta cggcctgctg ttcgagcgct tcctgaacgc
     7201 cgagcgcgtg tcgatgccgg atatcgacat cgacttctcg gtgaagggcc gcgagcgcgt
     7261 gatccgctac gtggcggcca agtacggcca ggactcggtc gcgcagatcg tcacgttcgg
     7321 ccggatgttc ccgcgcgcgg ccacgcgcga cgcagcgcgg gtgcttgggt tcgactacgg
     7381 cgccggcgac cggctggcga agctgatccc cgacccgatc atgggccgcc cgccgtcgtt
     7441 cgaggactgc ctgaagccgg gggaggagct ggcgcgcgcc tacaaggagg acgcgcaggc
     7501 gaagcagatc gtggacgtgg cgcgcgggct cgagggcgtg gtgcgcaact cgtcgatcca
     7561 cgcggcggcc gtggtgatcg ccgaccgccc gctcaccgac atcgtgcccc tccagctcgc
     7621 cgaggaccgc ggcgaggtga acgaggacgg cggccgcgcc taccggacgg tcacgcagta
     7681 ttcgatgaag ccgatcgagg agatcggcct gctcaagatg gacttcctcg ggctgcgcaa
     7741 cctcgacgtg atcgaggccg cgctcgacat cgtggagcgc tcgtccggcg agcggcccga
     7801 catggcgaag ctgccgctcg acgacgcgaa gacctacgag atgctcgccc ggggcgactc
     7861 gatcggcgtc ttccagttcg agtcggaggg catgcgcgag gcgctcaaga aggtgcgccc
     7921 gaccgagttc gaggacctcg tcgcgctcgt ggccctctac cgcccgggcg cgatgcgcca
     7981 catcgacacc tacgcgcgca acaagcgcaa cccggacgcc atcaactaca tcgacgaccg
     8041 cctgcgcccg atcaccgaca ccacctactc ggtgatcctc taccaggagc agtcgatgca
     8101 gatcgccaag gagatcgccg ggttcaccgg gccccaggcg gacgacctgc gcaaggcgat
     8161 cggcaagaag gaccgggcca agatggcctc gatgaaggac ctgttcgtgc agggcgcccg
     8221 ggcgaccggc acggccgacc gggtgatcca ggacctgtgg gagaccaacg aggcggcggc
     8281 cgactactcg ttcaacaagt cgcacgccgc ctgctacgcg ctgatcgcgt accgcacggc
     8341 gtggctcaag gccaactacc cggccgagta catggcggcg ctgatctcgt cggtcatgtc
     8401 gaccaaggac aaggtgccgt tcttcgtctc ccgctgcgag gagatgggga tcgaggtgct
     8461 gccgccggac gtgaacgagt ctgggcacga cttcgtggtc accgcgggca acatccgctt
     8521 cgggctcgac gcggtgaaga acgtgggcgc tgccgcggtc gacgcggtga tgaccgcgcg
     8581 cgaggaggac ggtccgttca cgtcgatctg ggacttctgc gagcgggtcg actgccagcg
     8641 ggtgaacaag aaggcgatcg agtcgctggt ccgctgcggg gcgttcgact cgctgggagc
     8701 ctcccggcgc gggatgctcg agatcctgcc ccaggcgcag gccgccgggc agaaggtgca
     8761 gcaggacgcg cagatgggcc aggcctcgat cttcgacctc gacgaggggc cggcggcgat
     8821 gcaggcaccg cgccagcacc cgcccgtgcc gctcatcgag gacgagcgcg ccgagctgaa
     8881 cgccatggag aaggagaccc tcgggctctt cctgtccagc cacccgttca aggaggtccg
     8941 ggccgcgctg cgcctgaagg tcgactgcgg cctcgcggac ctcgcgaaga agcgtgacgg
     9001 cgagtgggtc acggtcggcg gaatgatcgc cgaggccaag aagatcagga cgaagaaggg
     9061 cgacccgatg ctgttcgcca ccctggacga tctcgaggcg caggtcgaga tcctcgtctt
     9121 cagcaaggcc tactcggcga acgcggagaa gatcgacacc gacaacgtcg tgatcgtccg
     9181 tgggcgggtc gaccacaagg agcagggcga gaccaaggtg gtggtccagg agtgcgagcc
     9241 gttcgagccg acgcccgacg aggtcgagcg ggcccgcgag caggccgcgg tgatcctcgc
     9301 gcccccgcgt ttgaagctac gcgtgtcgcc ggggatcgac gccggctggc tagaggacct
     9361 gaaggagctc gtcaagcaca acaaggggga tcacgagctg gtcgtctgcg tgggcgaccg
     9421 cacgctcgtc ctcggcgagc agtaccgcgt gtcacccggc cgcttctgcg cggagctcca
     9481 ggagctcccg ggcgcgccgg agctggtgta gccccggcgc gcgacggtcc gctgcgttac
     9541 tgctggtcgc cggtcggctg caccacgccg acgaggtgtc cctcgggatc cttcaggtgg
     9601 ccgagcacga tgccagcgcc ggggacctcg tccgggcccc agacgcgggt gccgccgagc
     9661 tcctcggcct tggccaggta cgcctcggcg tcgtccacgc cgacgtagaa ggtcacgtgg
     9721 ccgtcggcct ccgacgagcc gccgatcccg ccgccgatcc cgatgccctc cgggctgagg
     9781 ttctgctcgc gatcgacggt cgcgtagtcc atcggccctc cgacctcgtg gagctcccag
     9841 ccgaacagct cgctgtagta gctgcgcagc ttctgcccgt ccttgccgat gacctcgaag
     9901 tgcatgaccg gctgtcccat gtgtcctcct ccgtcgcgat tgttcacgtc tgtcccatag
     9961 atccggcgcg cccgcgaaat taatcgccgc gggcggattc tgctggcacc cgggcggacg
    10021 ccgctccggc accgtcaccc gaactgggca accttttggt cgtatttgac acctaacgtg
    10081 acccaggtct tcggccggca ggcgtaagct gccgtcatgg cgcagaccgc gcacccgggt
    10141 cagctcagcg agtgccgtca gtgctgctcc ttctgcgacc gggtcctgct cccatcgggc
    10201 tgcctcgact cgaactgcca gtacctctat ctctacgacg acgaggacaa cggccgccgc
    10261 tacatgggct gcctgaacaa ggtcttcaag gtggagatcg acctcgagct gttccgggag
    10321 tccgagcgca cccggcacgg cttcggggga gtgaagctca ccggcacgcc gatcccacgc
    10381 tgccggacgg cggtcgagcg cgcctaccac ggcggcggcg acgccttcga ctgcgtcaac
    10441 ccgatgttct tcgacgaccc ggccccaccg gccgcggact tcgacctgcg cgacgcgctc
    10501 tagccggccc gcccgccgcc tccggggggc gtccctccat cgattggagc cttgaccacg
    10561 ctatagcgga gtaaccctcc aatcgatgaa aaccggcgcc gccggagccg gctgtcctaa
    10621 gtgctcgcct ttgacgagcg cttggcggag ccgccggcct tcggcgccgc ggcggccttc
    10681 ggcttcggct tcgcggcggc cttcggcttc gcggagccgg ccttcggcgc cgcggcggcc
    10741 ttcggcttcg cggagccagc cttcggcttg gcgccggcct tcggcttcgc cttcgagccg
    10801 cctcccttcg gtgctgccgt tctcgcggag ccgcgcggct tggccgccgc cttgggcttg
    10861 gccgccgcct tgggcttggc cgccgccttc ggcttggcct tcgcgccggc cttccccttc
    10921 gccaccttgg ccttcgcctt cggcttggcc ttcgcctccg gcttcccatg cggcgtccgc
    10981 tccaccaccg gcagcccccg cgccgccagc ccctcgttcc gcagccggcg cgccaccgcc
    11041 atgtcctcgt aagccgcgcc cttcccggcg aggcccggcc cctcgaagat cgccggcagc
    11101 ccctcgaagc gcggctcgct gaagaacgcc gcgcaaccct ctggccccag ctcgccgtcg
    11161 cccagaggcg cgtggcggtc gcggttcgag cccagcggca tcattgagtc gttcacgtgc
    11221 aggcagcgca ggcggtcgag gtcgagcgac gccacgcact cgtccagcac ctccgagagt
    11281 ccctcggcgg tgcgcacgtc gaaccccgac gccagcatgt ggcacgagtc gaggcagatc
    11341 cccaaccgcg gcccgccgcc gcccagctcc agcagctccg ccagctcccc gaatgagcgc
    11401 ccgatcgtcc caccggcgcc agcggtgttc tccagtagga gcatgcagtt ctcggactcc
    11461 tcgagcacat gccggacggc ctcgcccacg cgccggaggg actcgcccag aggctcaccc
    11521 acggtcgagc ccggatggac tacgacgccg gacgccccga tcgcatcacc gacccgcagg
    11581 gcatgcgaca aagaagccag cgacttctcg cgaatgaccg ggtccttcga ggcgcagttg
    11641 atcaggtaca ccgcgtggat cacgatcgac gtgaccggcc cggcggcgag cagctcgcgg
    11701 aactccgcga tgtcgtcgtc gcggtagttc gtcggccgcc acatgcgcgg cgactggttg
    11761 aagatctgga tcgcgtcgca gtcgcgctcc accccgcggt ggaaggcgtt cacgagaccg
    11821 cccgccggtg agacgtggcc gccgattagc atcgcggtgt ggcagaaggc atgaggctga
    11881 ggttcgcgcc ctccccgacg ggggcgctgc acatcggcgg ggcgaggacg gcactgtaca
    11941 actggctgct ggcccgccag tcgggcggcg cgttcgtcct gcgcatcgag gacaccgacc
    12001 gcgagcgctc cacgccggag aacgtcgcgc agatccacga cgcgctcgac tggctggagc
    12061 tcgacgtcga cgagggccct cactcccaga gcgagcgccg cccccgccac gaggaggaga
    12121 tcgcgcgcct gctgggcgag gaccacgcct actacgacga gggcgcggtg cgcctgcgcg
    12181 tgccggacgg cgggcagacg atcgtgaacg acgtgatccg cggggagatc gtcttcgacc
    12241 acgaggcgat cgacgacttc gtcatcgcgc gctcggacgg gagcccgctc tacaacctcg
    12301 ccgtcgcggt cgacgatcgc gacatgggaa taacccacgt cgtccgcggg gcggaccacc
    12361 tctccaacac cccgcgccag ctcatgatcc tgaaggccct cggcgccgag ccgcccgtct
    12421 acgcccacgt gccgctcctt cacggcaccg acggcaagaa gctctcgaag cgtcacggcg
    12481 ccgccagcgt ccaggagctg cgcgcggccg gctacctgcc cgaggccgtc cgcaactacg
    12541 tcgccctgct cggctggggc tacgacgaga gcaccacctt cttcaccacc gaggagctga
    12601 tcgagcactt ctcgctcgag cgcgtctcga agtcgcccgc cgtcttcgac gagcagaagc
    12661 tccgctggat gaacggccgc taccagcgcg agatgacgct cgacgagctg cacgcccgcc
    12721 tcgagcgctt ccacgaccgg ccgctgccgc gcgaggcggt ggcgatctcc caggagaaga
    12781 tgcagacgct cgcagagttc tggccgctcg cgggcttcct ggtcgagccg cagcccaccg
    12841 acgagaaggc ctggaagaag gtcatgggcg acggcgcggg ggagaggctc gcgcgcgcac
    12901 gcgaggcgat cgcgagcgtg gagcctttcg agcccgaggc gctcgagtcg cggctcgcgc
    12961 cgctggtcga cgagctgggc gtgaaggcac gcgacctgta ccagccgatc cgcgtcgcga
    13021 tcagcggaag caccatctct ccgggcattt tcg
//
LOCUS       DASVPL010000086         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_10792, whole genome shotgun sequence.
ACCESSION   DASVPL010000086 DASVPL010000000
VERSION     DASVPL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10792"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VF517_03925"
     CDS             <1..459
                     /locus_tag="VF517_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052116.1"
                     /translation="AVAMTVTVSWAPPDAEALAALVYKQRRTGFGAGPVGAFIQPARE
                     RGAQLRVDRDVADVLVFAEDPQEGFAGGAGDVVDVEGDDLGDPRAGVERDERERLVAR
                     RRAALDFAEERSWARWSSAAAPWRRSRRGRRSRARGRGGCRSRRRRRARC"
     gene            643..1725
                     /locus_tag="VF517_03930"
     CDS             643..1725
                     /locus_tag="VF517_03930"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX8052117.1"
                     /translation="MAKARSLVGLDVHAAKIVAAVLDAETGELQYFTLGGDVIEAAGL
                     CAGLPRPVRVAYEAGPTGYGLARELRRRKVACVVAAPSKIPRAPGDRVKTDRRDAELL
                     VRLLWAGKLHAVRVPGPEEEALRDLVRAREAVRVDLMRCRHRLSKLLLRHGVRFDDGP
                     AWTQRHRDWLAQVQLGWPAAQATMLDARGAIDVLAHRRDALEREITALLPASVWAVQV
                     GRLRCLRGIDTLSAVGLCAEIGDFERFARAEQLMSYVGLVPCESTTGQQRRLGAITKT
                     GSGHARRLLIEAAWHYRPRPRIGKTLAERHSGQPAEAVAVAWSAQQRLHRTWTRLEAR
                     AKRRTIIAVAAARELVGFCWAITRIE"
BASE COUNT          248 a          606 c          728 g          272 t
ORIGIN      
        1 gccgtggcga tgacggtgac ggtgtcgtgg gcaccgccgg acgctgaggc gctggccgcg
       61 ctggtttaca aacagcgccg gactggtttt ggcgccgggc cggtcggcgc gttcatccag
      121 ccagcccgcg agcgcggcgc gcagctgagg gtggatcggg acgtggcgga cgtgttggtc
      181 tttgccgagg atccgcagga aggctttgcg ggcggagcgg gagatgtcgt cgacgtcgag
      241 ggcgacgatc tcggtgatcc gcgcgccggc gtagaacggg atgagcgcga gcgcctggtc
      301 gcgcggcgac gggcagcgct ggatttcgcg gaggagcgca gctgggcgcg ttggtcgagc
      361 gccgcggcgc cgtggcggcg atctcgacgc gggcggcgct cgcgggcccg aggccgcggc
      421 gggtgtagaa gtcgtcgacg gcggcgagcg cgttgttgac ggtcgcgggg cttcgcttga
      481 ggaccgtttg gacgtgcgtg cgggttagac catatggatt gtcggtccgt ccggcccagg
      541 cggtagaagg ggccggttcc cccacgacgg tcatgggcgg atgttcgtgc cgcgcggctc
      601 ctaccccgcg cggtgaccca caatcgcaga ggagaaccag ccatggcgaa ggctagaagc
      661 ctcgtcggtc tggacgtgca cgccgcgaag atcgtggcgg cggtgctcga cgccgagacc
      721 ggggagttgc agtacttcac gctgggcggt gacgtcatcg aggcggccgg gctgtgcgcg
      781 gggctgccgc gtccggtgcg cgtggcctat gaggccgggc cgacggggta cgggttggcg
      841 cgtgagctac gtcggcgcaa ggttgcgtgt gtggttgcgg cgccgagcaa gatccccagg
      901 gcgcctggtg acagggtcaa gaccgaccgc cgcgatgccg agctgttggt caggctgctt
      961 tgggccggca agctgcacgc ggtccgggtt ccgggccctg aggaggaggc gttgcgcgat
     1021 ctcgtccggg cgcgtgaggc ggtccgcgtg gatctgatgc gctgtcggca ccggctgtcc
     1081 aagctcttgc tgcgtcacgg cgttcgcttt gacgacggtc cggcgtggac gcagcgtcac
     1141 cgcgactggc tcgcgcaggt gcagctgggc tggccggcgg cgcaggcgac gatgctcgac
     1201 gcccggggcg cgatcgacgt gctcgcccat cgccgcgacg cgctggagcg cgagatcacc
     1261 gcgctgctgc cggcctcggt gtgggcggtt caggttggca ggctgcgctg cctgcgcggg
     1321 atcgacacgc tgtccgcggt cggactgtgc gccgagatcg gcgacttcga gcgcttcgcc
     1381 cgcgccgagc agctgatgag ctacgtcggg ctcgtgccct gcgagagcac gaccggccaa
     1441 caacgccgac tcggcgcgat caccaagacc ggctccggcc acgcgcggcg gctgctcatc
     1501 gaggcggcct ggcactaccg gccgcggcca cggatcggca agacactcgc cgagcgccac
     1561 agcggacagc ccgccgaagc ggtcgcggtc gcctggagcg cccagcagcg cctgcaccgc
     1621 acctggacac gcctggaggc gcgcgccaag cgacgcacga tcatcgccgt cgccgccgcc
     1681 cgcgaactcg tcggcttctg ctgggccatt acccgcatcg aatgacgcgc cggcacccga
     1741 ccccataact tcccgtcggc tgggtcggtg gcggcccggc aacgcgcggg gaatccgcga
     1801 cgcgactatg agcaacccgc gccccgcaac cggggcgctg gccacgcccg atgt
//
LOCUS       DASVPL010000087        11142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_49957, whole genome shotgun sequence.
ACCESSION   DASVPL010000087 DASVPL010000000
VERSION     DASVPL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11142
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49957"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VF517_03935"
     CDS             <1..1385
                     /locus_tag="VF517_03935"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX8052118.1"
                     /translation="ELAVAAQRLAARLLVELCGARLVPGTLDEYPRPAEHREVELRPE
                     RCEKLLGERVPPEQVEAILGRLGFAPRAAGERIAVTVPPWRDSDVQREADLIEEVARV
                     HGLDKLPVTLPAREHAVGRLTPAQRLRRRLEDVLRDRGLYECVAYSFVAPATLARLRA
                     DATDVLRVENPLSEELSVMRPLLLPGLLDAARHNAAHGRGGVALFESAHVYAPAGPLA
                     GAPAGSPHGALPAGESHHLAALLTRALPATWRSPEREADYFAARALLDALMDAAGVEW
                     EPRAGGEPDGTADGEADAGGAGAAPPAGWLHPGRSARVLAGGRDVGWIGEVHPSVARE
                     WDLDGAVAGFEVDFDALAELASGPAYYTDVTTFPSVLLDIAVVLPADVSADRAEAAVR
                     AGGGELLGSARVFDVYTGEQVGEGKRSLALRLEFRAPDRTLTDDEVAELRAAIERELA
                     ELGGSLRARS"
     gene            1372..2367
                     /gene="argC"
                     /locus_tag="VF517_03940"
     CDS             1372..2367
                     /gene="argC"
                     /locus_tag="VF517_03940"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX8052119.1"
                     /translation="MPGAEATGRRVAVAGAAGFAGAVAAHLVQRHPSLELSAVTARSD
                     AGRKLDSIYPRYRVGLELEAFDADRIAERSDAAIVAYPHGAAAPVVEALRRRGLKVVD
                     LSADFRLGRAAYERYYQPHEAPALLDEAVYGLTELHRDAIRDADLVAAPGCYPTAAIL
                     ALAPLRELIADVIVDAKSGVSGAGREATETTHFVMVDENINPYKVEGHRHRAELEQEI
                     GGTITFTPHLLPIDQGLLASCYVTPTRDLSAAELRDLFAAAYDDEPFVELVDSPPGVR
                     EVRETNFCRVHATVEPATGRVLVFAAIDNLWKGAAGQAVQDLNLMLGLPEGEGLT"
     gene            2364..3614
                     /gene="argJ"
                     /locus_tag="VF517_03945"
     CDS             2364..3614
                     /gene="argJ"
                     /locus_tag="VF517_03945"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX8052120.1"
                     /translation="MTFFRSRWVTRPPHVRELEPTALPAGFRAGAAAAGLKAEGLDVG
                     VLVSDEPGTTSAARFTTNARVGAPVTVSREAALDRLRAVVANSGGSNTGDGERGIATA
                     EAAQARAAELLGVEPEQVGVASTGVIGAELPRDKLLTGVDAAIDALGPDAAGLAEAIV
                     TTDRGPKRACLEVVLPSGASVRIAGQAKGAGMISPRFATMFCFVQTDAALEQETVELL
                     TGVCVKRSFDRISVDGQLSTSDTVFVLASGASGVEVAPESDDEVALGEAMDALMRQLA
                     VEIVADGEGSERIGRVVVRGDAAAVEPVARAVANSPLVKTALHGADPNFGRILQAAGM
                     AWSAVADGSPMYVDLDIEGHTVVSGNAAAIDFGGPEWSELEQAVRVSEVEYVLTLPGE
                     GAETEVFFSDLGHEYIRINAEYTT"
     gene            3628..4467
                     /gene="argB"
                     /locus_tag="VF517_03950"
     CDS             3628..4467
                     /gene="argB"
                     /locus_tag="VF517_03950"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX8052121.1"
                     /translation="MRDIATLLEALPYIREFHEKTIVIKYGGAAMTDEELREDFARDV
                     VLLKYVGMNPIVVHGGGPDITSYMKRLGMHVEFVEGLRVSDAETVEIAKMVLVGKQNK
                     DIVLRLNRFGQPAVGLCGDDGLLFRAEKQFAGERDIGFVGRIERVDVNVLNHIAEDYI
                     PVIASVGADAEGQSYNINADEAAAAVAEAMGAEKVIFLTDVRAWLEDPADPATRISQA
                     TAAEVRAKLEGVTGGMRPKLTACAHAVENGVSAAHIIDGRTPHSLLLELFTDAGIGTK
                     IFP"
     gene            4464..5618
                     /locus_tag="VF517_03955"
     CDS             4464..5618
                     /locus_tag="VF517_03955"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX8052122.1"
                     /translation="MTLADLQSLERDHLMPTYARQPVEFVRGEGTVLWDSDGNEYLDF
                     LAGISVVQIGHCHPALVEAVSAQAARLMHVGNLYYTEPGLRLARRLGERSLGGKVFFA
                     NSGAEANECAIKLARKHRRGGEIVVFEGGFHGRTMGALSATPQETKQEPFAPLVPGFV
                     TIPRGGSLAGAVTERTAAVLVEPIQGESGIWEIPEATLAEARELCDRTGALLIFDEVQ
                     CGMGRTGSLWAYEQYGIRPDVMTLAKGLGGGLPIGACVTTPEHADVLAAGDHGSTFAG
                     GPVPAAGANAVLDVVDDEGFLAEVDRKGAALSGGLARLGLDVRGRGLMLAFATGDGPG
                     LARRALLEERLVVNATGPDTVRLLPPLTVSDEEIDAAVERIARLVGGPAR"
     gene            5633..6907
                     /locus_tag="VF517_03960"
     CDS             5633..6907
                     /locus_tag="VF517_03960"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX8052123.1"
                     /translation="MAHDRRTLIKTASYEAEPDDVGRVLLLYSGGLDTSVMLKWIQDE
                     YKAEVVALTVNLGQPDEDWDVIRGKALDIGAVDCMLVDAREEFVEDYVLPAIKANALY
                     GGGYPLFTALGRPLIARLAVEHARATGCDTIAHGCTGKGNDQVRIEATIATLAPELKI
                     IAPVRGWQMGREEEIAYAREHGIPVKGGTEAPPYSIDDNLWGRSSEGGPIEDLDEPAR
                     DDVFELVTLPQNAPDEPELVPIEFERGRPVAIGGERLGLVDLLERAGEIGARHGIGIV
                     DHIEDRIVGLKVRDIYEVPAAELILTAHKELEKLVGTIHQNQFKPGLDHHWAFLVYAG
                     LWHEPLLGDLNAFMDRVNEQVTGTITLKLYKGSAKPVARSSPNALYDAALAGFGESGG
                     LFSQQASPGFIELWSLQSRMAYGIRNRVQEDA"
     gene            6904..7068
                     /locus_tag="VF517_03965"
     CDS             6904..7068
                     /locus_tag="VF517_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052124.1"
                     /translation="MNSKGYTVLGWVVWQVVSRVAKRKVRQNRTKVGAAAAVLLVLVL
                     GLAAARSSSD"
     gene            7222..>11142
                     /locus_tag="VF517_03970"
     CDS             7222..>11142
                     /locus_tag="VF517_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052125.1"
                     /translation="MVLAALASTASARTFSQRFGEVARGDLSIAANSSLSCPSSGSTN
                     GNSCYQTRNGTGNVLDNAGVVMTNVDVDSDGATFNSTTSDLTLPAGATVLYAGLYWGA
                     DSSGGAGGAAPPNAAARDTVRFKPPGAASYSTVAAAQLDTDPATANRYQGFADVTTQV
                     QAAGAGTYTVANIQAGTGNDRYGGWTLAVAYKATGLPVKWVALYDGMLSIGSGGTANT
                     ASITLDGFTAPASGTVAAELGLAGYEGELAYAGETMALGATTIGSGARPATNPFNASV
                     TNLGANVTTRNPAYVNNLGFDAGIMAADGAIAPNATSATLALTSPTDLFLPGLVTLVS
                     DHEASAPANTALPSISGTTQLGQTLTASSGTWTGTSPITYAYQWQRCNSSGASCANIA
                     GATNLTYTVAAGDVGSTFRVAVTATNVVSSTSATSNATATATGVPPTNTGLPTLSGTT
                     QDGQTLSSTNGSWSGTAPFTYAYQWRRCDSAGVNCANISGATASTYALTPADIGSTVR
                     SVVTATNSGGSQSATSAQSAVVTAIPLANTALPAITGTPQHGQTLTASNGTWTGSPTI
                     TYAYQWRRCDSTGANCVNISGATGSTYVVQQADVTSPASTIRVVVTATNPGGSQSATS
                     TQTAGVSAALPVNTALPAITGTPQHGQTLTASNGTWTGTTPQTYAYQWRRCDSSGASC
                     VDISGATSQTYVVQQADVTSPASTLRVVVTATNAAGSQSATSAQTATVTAAPPVNTGL
                     PTVTGTAQEGQTLSSTNGSWSGTAPFTYAYQWRRCDASGASCVNIAGATGSTYVVQQA
                     DVTSPASTLRAVVTATNSAGNAAATSAQTASVTGAPPVNTALPAISGSAQHGATLTAS
                     TGTWSGSTPIAYTYQWRRCDASGASCSSISGATSSSYVVQQADVTTPASTIRVVVTAT
                     NTAGNAAATSAQTASVTPAPPANTALPTISGTPQHGQTLTASTGTWTGTPTISYVYQW
                     RRCDSSGATCSDISGATSSTYVVQQADVTTPASTIRVRVTASNAGGFSSANSAQTTAV
                     AAAAPTNTSPPSISGTTQDGQTLTSTNGSWSGTAPFTYTYEWRRCDSAGANCVSISGA
                     TASTYALTPADIGSTLRSVVTATGPGGTQSSTSSQTAVVTAIPLANTAAPTISGTPQD
                     GQTLTASNGTWTGSPTITYAYQWRRCDASGASCVNISGATASTYVAQQADVTGTLRVL
                     VTATNPGGSLAATSAQSTTVAASPPVNTSLPAISGTPQHGQTLTASSGTWTGTTPITY
                     AYQWRRCDSAGANCANIAGATASTYVVQQADVTTPASTIRVVVSA"
BASE COUNT         1620 a         4245 c         3907 g         1370 t
ORIGIN      
        1 cggagctcgc ggtagcggcc cagcggctcg cggcgcggct gttggtcgag ctctgcgggg
       61 cgcgactcgt gccgggcacg ctcgacgagt acccgcgccc ggccgagcac cgcgaggtcg
      121 agctgcggcc ggagcgttgc gagaagctgc tcggcgagcg cgtgccgccg gagcaggtcg
      181 aggcgatcct cgggcggctc gggttcgcgc cgcgcgccgc gggtgagcgg atcgcggtga
      241 ccgtgccgcc ctggcgcgac tccgacgtgc agcgcgaggc cgacctgatc gaggaggtcg
      301 cgcgcgtgca cggcctcgac aagcttccgg tcaccctgcc ggcgcgcgag cacgccgtcg
      361 gccggctcac gcccgcgcag cgcctgcgcc gccggctcga ggacgtgctg cgcgaccgcg
      421 gcctgtacga gtgcgtggcg tattcgttcg tagcgcccgc gacgctcgcg cgcctgcgcg
      481 cggacgcgac cgacgtgctg cgcgtcgaga acccgctgag cgaggagctg agcgtgatgc
      541 gcccgctgct cctgcccggg ctgctcgacg cggcgcgcca caacgccgcg cacgggcgcg
      601 ggggcgtggc cctgttcgag tccgcgcacg tgtacgcgcc ggccggcccg ctcgccggcg
      661 ctccggcggg ctcgccgcac ggagcgctcc cggccggcga gagccaccac ctggccgcac
      721 tgctcacccg cgcgctcccg gcgacctggc ggagccccga gcgcgaggcc gactacttcg
      781 cggcgcgcgc cctgctggac gcgttgatgg acgccgcggg cgtcgagtgg gagccgcgcg
      841 cgggtggcga gcccgacggg accgcggatg gggaggccga cgccggcggc gcgggcgccg
      901 ccccacccgc cggctggctc caccccggcc gctccgcgcg ggtgctcgcc ggcggccgcg
      961 acgtcggctg gatcggcgag gtccatccga gtgtcgcgcg cgagtgggat ctcgacggcg
     1021 cggtcgcggg attcgaggtc gacttcgacg ccctagcgga gctcgcgtcc ggccccgcgt
     1081 actacaccga cgtgaccacc tttccgtcgg tgctcctcga catcgccgtg gtactgcccg
     1141 ccgatgtgag cgcggatcgt gccgaggcgg ccgtgcgcgc gggcggcggc gagctgctcg
     1201 gctccgcccg cgtgttcgac gtctacacgg gcgagcaggt cggggaggga aagcggtcgc
     1261 tggcgctgag gctcgagttc cgcgcccccg atcgcacgct caccgacgac gaggtcgccg
     1321 agctgcgcgc cgcgatcgag cgcgagctcg ccgagctggg agggagtctc cgtgccagga
     1381 gctgaagcca ccggacgcag ggtcgccgtc gcgggcgcgg ccgggttcgc cggcgccgtc
     1441 gccgcccacc tcgtccagcg ccatccctcg ctcgagctga gcgccgtgac cgcgcgctcg
     1501 gacgccgggc gcaagctcga ctcgatctac ccgcgctacc gcgtcgggct cgagctcgag
     1561 gccttcgacg ccgaccgtat cgccgagcgc tccgacgccg ccatcgtcgc ctacccgcac
     1621 ggagccgccg cgccggtggt cgaggcgctg cgccgccgcg gcctgaaggt cgtggacctc
     1681 tcggccgact tccggctcgg ccgcgcggcc tacgagcgct actaccagcc gcacgaggcg
     1741 cccgccctgc tcgacgaggc cgtgtacggc ctgaccgagc tgcaccgcga cgcgatccgc
     1801 gatgccgacc tcgtggccgc gcccggctgc tacccgaccg ccgcgatcct ggcgctcgcc
     1861 ccgctgcgcg agctgatcgc cgacgtgatc gtggacgcga agtccggcgt ctccggcgcc
     1921 ggccgcgagg ccaccgagac gacgcacttc gtgatggtgg acgagaacat caacccgtac
     1981 aaggtcgagg gccaccgcca ccgcgccgag ctcgagcagg agatcggcgg cacgatcacg
     2041 ttcaccccgc acctgctccc gatcgaccag ggactcctgg cgtcgtgcta cgtcacgccc
     2101 acgcgcgacc tctccgccgc cgagctgcgc gacctcttcg cggccgccta cgacgacgag
     2161 ccgttcgtgg agctggtcga ctcgccgccg ggcgtgcgcg aggtgcgcga gaccaacttc
     2221 tgccgcgtcc acgccaccgt cgagccggcc accggccgcg tgctcgtgtt cgccgccatc
     2281 gacaacctct ggaagggcgc ggccggccag gccgtgcagg acctcaacct catgctcggg
     2341 ctgccggagg gggaggggct cacgtgacgt tcttccgctc ccgctgggtc acgcgccccc
     2401 cgcacgtgcg cgagctcgag cccaccgcgc tgccggcggg cttccgcgcc ggcgccgccg
     2461 cggccggcct caaggccgag ggcctcgacg tgggcgtcct ggtctccgac gagcccggca
     2521 cgacctccgc tgcccgcttc accaccaacg cgcgcgtggg cgcgcccgtg acagtctcgc
     2581 gcgaggcggc gctcgaccgg ctgcgcgcgg tggtggccaa ctcgggcggc tcgaacaccg
     2641 gcgacggcga gcgcggcatc gccacggccg aggccgcgca ggcgcgcgcg gcggagctgc
     2701 tgggcgtcga gccagagcag gtcggcgtgg cgtcgacggg cgtgatcggc gccgagctgc
     2761 cgcgcgacaa gctgctgacc ggtgtcgacg ccgccatcga cgcgctcggc ccggacgccg
     2821 caggcctggc cgaggcgatc gtgaccaccg accgcgggcc gaagcgcgcc tgcctcgagg
     2881 tggtgctgcc gtcgggcgcg agcgtgcgca tcgccggcca ggccaagggc gccgggatga
     2941 tctcgccgcg cttcgcgacg atgttctgct tcgtgcagac cgatgccgca ctcgagcagg
     3001 agaccgtcga gctgctgacg ggcgtctgcg tgaagcgctc gttcgaccgg atctcggtgg
     3061 acggtcagct atcgaccagc gacacggtgt tcgtgctggc gagcggcgcg tcgggcgtcg
     3121 aggtggcgcc cgagtccgac gacgaggtgg cgctcggcga ggcgatggac gcgctgatgc
     3181 gccagctcgc cgtcgagatc gtggccgacg gcgagggctc cgagcgcatc ggcagggtgg
     3241 tcgtgcgcgg cgacgcggcc gccgtcgagc cggtggcccg cgcggtcgcc aactcgcccc
     3301 tcgtaaagac cgcgctgcac ggcgccgacc ccaacttcgg gcgcatcctc caggccgccg
     3361 gcatggcctg gtcggcggtg gccgacgggt cgccgatgta cgtcgacctc gacatcgagg
     3421 gccacaccgt cgtgtccggc aacgccgccg cgatcgactt cggcgggccc gagtggagcg
     3481 agctcgagca ggccgtccgg gtgagcgagg tcgagtacgt gctgaccctg ccgggcgagg
     3541 gcgccgagac cgaggtcttc ttcagcgacc tcggccacga gtacatccgg atcaacgcgg
     3601 agtacacgac atgagggggc cgagctgatg cgcgacatcg cgaccctcct cgaggcgctc
     3661 ccctacatcc gcgagttcca cgagaagacg atcgtcatca agtacggcgg cgcggcgatg
     3721 accgacgagg agctgcgcga ggacttcgcc cgcgacgtgg tcctcctcaa gtacgtcggg
     3781 atgaacccga tcgtggtgca cggcggcggc cccgacatca cctcctatat gaagcgcctc
     3841 ggcatgcacg tcgagttcgt ggaggggctg cgcgtctccg acgccgagac cgtggagatc
     3901 gcgaagatgg tcctggtcgg caagcagaac aaggacatcg tgctgcggct gaaccgcttc
     3961 gggcagcccg cggtcggact ctgcggcgac gacggcctgc tcttccgcgc cgagaagcag
     4021 ttcgcgggcg agcgcgacat cggcttcgtg ggccggatcg agcgcgtgga cgtgaacgtg
     4081 ctgaaccaca tcgccgagga ctacataccg gtcatcgcat cggtcggggc cgacgccgag
     4141 ggccagtcct acaacatcaa cgccgacgag gccgcggccg cggtggccga agccatgggc
     4201 gccgagaagg tcatcttcct caccgacgtg cgcgcctggc tcgaggaccc cgccgacccc
     4261 gccacgcgga tcagccaggc gaccgcggcc gaggtgcgcg cgaagctcga gggcgtgacc
     4321 ggcggcatgc ggccgaagct caccgcctgc gcgcacgcgg tcgagaacgg cgtgagcgcc
     4381 gcgcacatca tcgacggccg cacgccgcac tcgctgctgc tcgagctgtt caccgacgcc
     4441 ggcatcggca cgaagatctt cccgtgacgc tcgccgacct ccagtcgctc gagcgcgacc
     4501 acctgatgcc gacctacgcg cggcagccgg tcgagttcgt gcgcggcgag gggacggtcc
     4561 tgtgggactc cgacgggaac gagtacctcg acttcctggc cggcatctcg gtcgtgcaga
     4621 tcgggcactg ccacccggcg ctggtcgagg ccgtgagcgc gcaggcggct cggctcatgc
     4681 acgttggcaa cctctactac accgagcccg ggcttcggct ggcacggcgc ctcggcgagc
     4741 gctcgctcgg cggcaaggtg ttcttcgcca acagcggcgc cgaggccaac gagtgcgcca
     4801 tcaagctcgc tcgcaagcac cgccgcggcg gcgagatcgt cgtgttcgag ggcggcttcc
     4861 acggccgcac gatgggcgcc ctctcggcca ccccgcagga gaccaagcag gagccgttcg
     4921 caccgctcgt gcccggcttc gtcacgatcc cgcgcggcgg ctcgctcgcg ggcgccgtga
     4981 ccgagcgcac cgccgcggtg ctggtcgagc cgatccaggg cgagtcgggg atctgggaga
     5041 tacccgaggc gacgctggcc gaggcgcgcg agctgtgcga ccggacgggc gcgctgctga
     5101 tcttcgacga ggtccagtgc ggcatggggc gcacgggctc gctctgggcc tacgagcagt
     5161 acggcatccg gcccgacgtg atgacgctcg ccaaggggct cggcggcggg ctgccgatcg
     5221 gcgcgtgcgt gaccacgccc gagcatgccg acgtgctggc cgcgggcgac cacggttcca
     5281 ccttcgcggg cggcccggtg ccggcggcgg gcgccaacgc ggtgctcgac gtggtggacg
     5341 acgagggctt cctggcggag gttgaccgca agggcgcggc gctctccggg ggcctcgccc
     5401 ggctcgggct cgacgtgcgc ggccgcggcc tgatgctggc gttcgcgacc ggcgatggcc
     5461 ccggcctcgc ccgccgcgcg ctgctcgagg agcggctcgt cgtgaacgcc accggccccg
     5521 acaccgtccg cctcctcccg ccgctcaccg tgagcgacga ggagatcgac gccgcggtcg
     5581 agcggatagc ccgcctcgtc ggcggaccgg cgcgctgata ggttcggcgc ccatggccca
     5641 cgaccgcaga actctcatca agaccgcctc ctacgaggcc gagcccgacg acgtcggccg
     5701 cgtgctgctg ctctactccg gcggcctcga cacgagcgtg atgctcaagt ggatccagga
     5761 cgagtacaag gccgaggtcg tggcgctcac cgtgaacctc ggccagcccg acgaggactg
     5821 ggacgtgatc cggggcaagg cgctcgacat cggcgcggtc gactgcatgc tggtcgacgc
     5881 gcgcgaggag ttcgtcgagg actacgtgct gcccgcgatc aaggcgaacg ccctctacgg
     5941 gggcggctac ccgctcttca ccgcgctcgg ccggccgctg atagcgcggc tcgcggtcga
     6001 gcacgcgcgc gcgaccgggt gcgacacgat cgcccacggc tgcaccggca agggcaacga
     6061 ccaggtcagg atcgaggcga ccatcgccac gctcgctccc gagctgaaga tcatcgcgcc
     6121 cgtgcgcggc tggcagatgg ggcgcgagga ggagatcgcg tacgcgcgcg agcacggcat
     6181 tccggtcaag ggcgggaccg aggcgccgcc gtactcgatc gacgacaacc tctggggccg
     6241 ctcgtccgag ggcgggccga tcgaggatct cgacgagccc gcgcgcgacg acgtgttcga
     6301 gctcgtcacg ctgccgcaga acgcgccgga cgagcccgag ctcgtcccga tcgagttcga
     6361 gcgcgggcgg ccggtggcga tcggcggcga gcgcctcggc ctcgtggacc tgctcgagcg
     6421 cgcgggtgag attggcgcgc gccacggcat cgggatcgtc gaccacatcg aggaccggat
     6481 cgtcggcctc aaggtgcgcg acatctacga ggtgccggcg gccgagctga tcctcaccgc
     6541 gcacaaggag ctagagaagc tcgtcgggac gatccaccag aaccagttca agcccggcct
     6601 cgaccaccac tgggcgttcc tcgtgtacgc ggggctctgg cacgagccgc tgctcggcga
     6661 cctgaacgcg ttcatggatc gcgtcaacga gcaggtcacc ggcacgatca ccctgaagct
     6721 ctacaagggc agcgcgaagc cggtcgcgcg ctcttcgccg aacgctctct acgacgccgc
     6781 gctggccggc tttggcgagt ccggcgggct gttctcgcag caggccagcc cgggcttcat
     6841 cgagctgtgg agcctccagt cgcgcatggc ctacggaatc cgcaaccggg tacaggagga
     6901 cgcatgaact cgaagggcta cacggtcctc ggctgggtcg tctggcaggt cgtctcgcgt
     6961 gtcgcaaagc gcaaggtcag gcagaaccgg acgaaggtcg gggccgccgc cgcggtcctc
     7021 ctggtgctcg tgctcggcct cgccgcggcg cgttcgtcct cggactagcg gccctcccgg
     7081 tcaagtcgcg gggcaccggg ccgataaggc cttggccccg gcgtacccat tttggggcca
     7141 gaccgcatgg ccccgaaccg accaacacac gctctccgcc cgcgcgccct ccggctcgcg
     7201 gccgcgtttg cgctcggttt ggtggtgctc gcggcactgg cctcgacggc ctcggcacgc
     7261 acgttcagcc agcgtttcgg cgaggttgcg cgtggcgacc tgagcatcgc ggccaactcg
     7321 agcctgtcgt gtcccagcag cggctcgacc aacggcaact cctgctacca gacccgcaac
     7381 ggcacgggca acgtcctcga caacgcgggc gtagtgatga cgaacgtcga cgtcgactcc
     7441 gacggcgcga cgttcaactc gaccacctcg gacctgacgc tgcccgcggg cgcgaccgtc
     7501 ctctacgccg ggctctactg gggcgccgac tccagcggcg gcgccggcgg ggccgcgccc
     7561 ccgaacgcgg ccgcgcgcga cacggtgcgc ttcaagccgc cgggcgccgc cagctactcg
     7621 acggtggccg cggcccagct cgacaccgac cccgccaccg ccaaccgcta ccagggcttc
     7681 gccgacgtga cgacccaggt gcaggccgcc ggcgcgggca cctacaccgt cgccaacatc
     7741 caggccggca ccggcaacga ccgctacggc ggctggacgc tcgccgtcgc gtacaaggcg
     7801 accgggctcc cggtgaagtg ggtcgccctc tacgacggca tgctctcgat cggcagcggc
     7861 ggcacggcca acaccgcctc gatcaccctc gacggcttca ccgcaccggc ctccggcacg
     7921 gtcgccgccg agctcggcct cgccggctac gagggcgagc tggcctacgc gggcgagacg
     7981 atggcgctcg gcgccaccac catcggctcg ggcgcgcggc cggcgaccaa cccattcaac
     8041 gcctcggtca cgaacctcgg ggccaacgtc accacgcgca acccggcgta cgtgaacaac
     8101 ctcggcttcg acgcgggcat catggccgcc gacggggcga tcgcgcccaa cgcgacgagc
     8161 gcgacactcg cgctcaccag cccgaccgac ctcttcctcc ccgggctcgt gaccctggtg
     8221 tccgaccacg aggcgagcgc gcccgcgaac acggcgctgc cctcgatctc gggcaccacg
     8281 cagctcggtc agacgctcac ggcctcgtcc ggcacctgga cgggcacctc cccgatcacc
     8341 tacgcctacc agtggcagcg ctgcaactcc tccggcgcca gctgcgcgaa catcgccggg
     8401 gccacgaacc tgacctacac ggtcgcggcc ggcgacgtcg gctcgacctt ccgcgtggcc
     8461 gtgaccgcca ccaacgtcgt cagctcgacg agcgccacct cgaacgccac cgccaccgcg
     8521 acgggcgtgc cgccgaccaa cacgggcctg cccacgctct cgggcacgac ccaggacggc
     8581 cagacgctca gctcgaccaa cggcagctgg agcggcaccg cgccgttcac ctacgcctac
     8641 cagtggcgcc gctgcgactc ggccggcgtg aactgcgcga acatctccgg cgccacggcc
     8701 tcgacctacg cgctcacccc ggccgacatc ggctcgacgg tgcgctcggt cgtgaccgcg
     8761 acgaactccg gcggcagcca gagcgcgacc agcgcccaga gtgccgtggt cacggcgatc
     8821 ccgctggcca acaccgcgct gccggcgatc acgggcacgc cgcagcacgg ccagacgctc
     8881 acggcctcga acgggacctg gaccggctcg ccgacgatca cctacgccta ccagtggcgc
     8941 cgctgcgact cgaccggcgc gaactgcgtg aacatctccg gcgccacggg ctcgacctac
     9001 gtcgtgcagc aggcggacgt gacctcgccg gcctcgacga tcagggtcgt cgtgaccgcg
     9061 accaaccccg gcggcagcca gagcgccacc agcacgcaga ccgccggcgt gagcgccgcc
     9121 ctgccggtga acacggcgct gccggcgatc acgggcacgc cgcagcacgg ccagaccctg
     9181 accgcgtcga acggcacctg gaccggcacg accccgcaga cctacgccta ccagtggcgg
     9241 cgctgcgact cctcgggcgc gagctgcgtc gacatctccg gtgcgacgag ccagacctac
     9301 gtcgtgcagc aggccgacgt gacctcgccg gcctcgacgc tgcgggtggt cgttacggcg
     9361 acgaacgccg ccggcagcca gagcgccacg agcgcccaga ccgcgacggt caccgcggct
     9421 ccgccggtca acaccggcct gcccaccgtc acaggcaccg cgcaggaggg ccagacgctc
     9481 agctcgacca acgggagctg gagcggcacc gcgccattca cctacgccta ccagtggcgc
     9541 cgctgcgacg cctccggcgc gagctgcgtg aacatcgcgg gtgccacggg ctcgacctac
     9601 gtggtgcagc aggcggacgt gacctcgccc gcctccacgc tccgcgccgt ggtgaccgcg
     9661 accaacagcg cgggcaacgc cgccgcgacc agcgctcaga ccgcgagcgt gaccggggcc
     9721 ccgccggtga acacggcgct gccggcgatc agcggctcgg cgcagcacgg cgccacgctc
     9781 accgcctcga ccggcacgtg gagcggcagc acgccgatcg cctacacgta ccagtggcgc
     9841 cgctgcgacg cctccggcgc gagctgctcg agcatctcgg gcgcgacgag ctcctcctac
     9901 gtggtgcagc aggccgacgt gaccacgccg gcctcgacga tccgcgtggt cgtgaccgcc
     9961 accaacaccg cgggcaacgc cgcggccacg agcgcgcaga ccgcgagcgt gaccccggct
    10021 ccgccggcca acaccgcgct gcccacgatc tccggcaccc cgcagcacgg ccagacgctc
    10081 acggcctcga ccggaacctg gacgggcacg cccaccatca gctacgtcta ccagtggcgc
    10141 cgctgcgact cgtcgggcgc gacctgctcg gacatctccg gcgcgaccag ctccacctac
    10201 gtggtccagc aggccgatgt gaccacgccg gcctcgacca tccgggtccg cgtgacggcc
    10261 tcgaacgccg gcgggttcag ctccgcgaac agcgcgcaga cgacggccgt cgccgcggct
    10321 gccccgacga acacctcgcc gccgtcgatc tcgggcacga cccaggacgg ccagacgctc
    10381 acctcgacca acggcagctg gagcggcacc gcgcccttca cctacaccta cgagtggcgg
    10441 cgctgcgact cggccggcgc caactgcgtg agcatctcag gcgccacggc ctccacgtac
    10501 gcgctgaccc cggcggacat cggctcgacg ctgcgctcgg tcgtgaccgc gaccggcccg
    10561 ggcggcaccc agagctcgac cagcagccag acagcggtcg tcaccgcgat cccgctggcc
    10621 aacacggcgg cgccgacgat ctcgggcacg ccccaggacg ggcagacgct cacggcgtcg
    10681 aacgggacct ggaccggctc gccgacgatc acctacgcct accagtggcg ccgctgcgac
    10741 gcctcgggcg cgagctgcgt gaacatctcg ggtgcgacgg cctcgaccta cgtcgcccag
    10801 caggcggacg tcacgggcac gctgcgggtc ctcgtcacgg ccacgaaccc gggcgggagt
    10861 ctcgcggcca cgagcgcgca gagcacgacg gtcgcggcta gccccccggt gaacacgtcg
    10921 ctgccggcga tcagcggcac cccgcagcac ggccagacgc tcacggcctc gagcggcacc
    10981 tggaccggga ccaccccgat cacctacgcc taccagtggc gccgctgcga ctccgcgggc
    11041 gcgaactgcg ccaacatcgc cggtgcgacc gcctcgacct acgtggtgca gcaggcggac
    11101 gtgaccacgc ccgcgtcgac gatccgcgtg gtcgtctccg cc
//
LOCUS       DASVPL010000088        38709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_127560, whole genome shotgun sequence.
ACCESSION   DASVPL010000088 DASVPL010000000
VERSION     DASVPL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 38709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38709
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127560"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /gene="rarD"
                     /locus_tag="VF517_03975"
     CDS             complement(<1..540)
                     /gene="rarD"
                     /locus_tag="VF517_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX8052126.1"
                     /translation="MNHGEAEHRRGIVYGTGAYLLWGLFPLYFPLLEPANPVEIVGHR
                     IVWSLVAVLVILALGAGFRPFLALLRDRRRAGLIALAAVLVAVNWGVYIYAVNSEHVI
                     EAALGYFINPLVSVLFGILVFRERLRPWQAAALILGAVAVLVLTLDYGRPPWIALILA
                     VTFGSYALVKKIADVGSAES"
     gene            595..1266
                     /locus_tag="VF517_03980"
     CDS             595..1266
                     /locus_tag="VF517_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052127.1"
                     /translation="MEATPPIPPPPPPPPTTALANPALHDHFVLRQRWAPFVNRYEFS
                     VPGHEDGAPELPVCFVQQKRFKFKEDIRFYSDASKTVELMRIKARQRFDPRARYDITD
                     PNGYKIGEIQKVFGESLLRSTFRVYDANGVETAKATEKSMAVALIRRFIGFVPWVGSF
                     ADWLPIPYHFVFLRGDHVLATNSRRLFKIRDTYDIDLTGDPQRTLDRRLVLAAVVGMD
                     ALQAR"
     gene            1314..2303
                     /locus_tag="VF517_03985"
     CDS             1314..2303
                     /locus_tag="VF517_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052128.1"
                     /translation="MGEALRAGFVAGTALGPLAPPLREEYIVSGLLTSGVPTLDTELR
                     RVLRPFGFDLTVGGVFCHGTPKVQLKGGGSCELADLLVVVRYENGGFVERNALLLQAK
                     KEGGAPPKGNQLRLYEDWPEFVYSSAAGLKGLKRSVHPKAPGPGAQFVELGTCPSCKK
                     WAAGCPVDGWAADSWRPGTARRPFLNELSDVIRGWSGRRFELPPRARTRGWDRVIEDL
                     LRVTAERAFRVAKRAMGSAGRGDVAHDYAQILREERCWPVQPRPLALYRDLGLSEEAA
                     DPSAWLSEVRVPPTLPPDALAGPVDDDDGAVSLLLVEISRGKDSRAVLSDEFT"
     gene            complement(2369..2614)
                     /locus_tag="VF517_03990"
     CDS             complement(2369..2614)
                     /locus_tag="VF517_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX8052129.1"
                     /translation="MRLLPRRRMTDVQEVAIRGDMIRLGQLLKLAGIVGGGGDAKALL
                     AEGHVHVNGEPESRRGRQLHAGDEITIGDVAIRVGGG"
     gene            complement(2587..2796)
                     /locus_tag="VF517_03995"
     CDS             complement(2587..2796)
                     /locus_tag="VF517_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052130.1"
                     /translation="MSPLAERVSEALRRDPLAGISASARDELAAALVAAQELEDLPGK
                     WQAAILRAESDAPDAPSCGCCHGGG"
     gene            2832..3170
                     /locus_tag="VF517_04000"
     CDS             2832..3170
                     /locus_tag="VF517_04000"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8052131.1"
                     /translation="MLKQRVPLDLTFQALADPTRRDMVERLSRGPASVSELAEPLTMS
                     LPAVVQHLQVLEAGGLVRSEKVGRVRTCHIEPDALGTAQQWIADRRTHWERRLDRLGD
                     VLADQPKGAE"
     gene            3167..3883
                     /locus_tag="VF517_04005"
     CDS             3167..3883
                     /locus_tag="VF517_04005"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX8052132.1"
                     /translation="MNIGTRDEWLAARVELLEREKEVTRLSDEVAEQRRALPWVPVEK
                     EYVFHTNDGPKTLAELFDGRSQLLVYHFMLGPNTPDGCPGCSFGADHWSGAVPHLNNH
                     DVTFILASRADLATINAYRERMGWTIPWVSSGGTDFNLDFSLFTEEQRASGTGFNFGT
                     PHRAKHHFDDMEVMALSTFVLAAGRVHHTYTTMDRGTDVLITTWQFLDRTPRGRFLVP
                     SEAEAAGGWPKRHDEYAQTT"
     gene            3880..4323
                     /locus_tag="VF517_04010"
     CDS             3880..4323
                     /locus_tag="VF517_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8052133.1"
                     /translation="MSVEHATFVLERDYPAPPARVFNAFADPELKQRWFGAPGWKWEM
                     DFREGGREHTSGEFEDGTAITFDSVYYDIVPDERIVYAYEMHSDGRRISVSVATFELG
                     PRGDGTRLRLTEQGAFLDGADTPGTREGGTVSLLDALGEVLASAA"
     gene            complement(4310..4630)
                     /locus_tag="VF517_04015"
     CDS             complement(4310..4630)
                     /locus_tag="VF517_04015"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8052134.1"
                     /translation="MELIEFPADDAERALSFWRGLLSLELDPRDSAEGSGWQTHGDGP
                     ALGIHERGRGPGDRFALPYFAVDDLDAALASVAELGGEVIHPGESWAVCRDSEGTPFG
                     LTQR"
     gene            complement(4641..4871)
                     /locus_tag="VF517_04020"
     CDS             complement(4641..4871)
                     /locus_tag="VF517_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052135.1"
                     /translation="MTRPSRPPPHVGMPVQVMRFGTVEPAVVEEVRDEGRTLVVAGET
                     YTLRPVNAHHVLEGEPSYGTRLLLGPGAEGGA"
     gene            complement(4886..6712)
                     /gene="typA"
                     /locus_tag="VF517_04025"
     CDS             complement(4886..6712)
                     /gene="typA"
                     /locus_tag="VF517_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX8052136.1"
                     /translation="MAARTDIRNVAIIAHVDHGKTTLVDAMLRQSGVFGAHEAVSDRV
                     MDSMDQERERGITILAKNAAVRYGEVKLNIVDTPGHADFGGEVERGLSMVDGVLLLVD
                     ASEGPLPQTRFVLRKALERRLPVILVVNKVDRPDARIGEVVDEVYELFLDLDADEAQI
                     EFPIAYTNARAGWASLDPGEEGTDLRPLLDILVEHVPAPEYEDGHGLQALVTNLDASP
                     YVGRLALCRIHNGVLRRGEAVAWCRRDGSVERAVVGEMYVAEGLGRVDAPEAGPGEIV
                     AVAGIPEITIGETLADPGDPRPLPPINVDEPALGMTIGINTSPLAGRSGSRLTARQVR
                     ERLDAELVGNVSIRVLDTERPDTWEVQGRGELQLAVLVESMRREGFELTVGKPQVVTR
                     EIDGKLHEPVERLAIDVPEDFLGVTTQLLALRKGRMEQMVNHGTGWVRLEYIVPARGL
                     IGFRTEFLTETRGSGILHSVFERYDPWYGDIKTRPTGSLVADRRGETGQFALLKMQER
                     GQLFVGPGEEVYEGMVVGENARADDLDVNAVREKKQTNIRAAGSDDTVRLVPHHQLSL
                     DQALEFIRTDECVEVTPDKLRLRKVVLPAGERQKLARSAKWG"
     gene            6757..7119
                     /locus_tag="VF517_04030"
     CDS             6757..7119
                     /locus_tag="VF517_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052137.1"
                     /translation="MSRAATIFAGPVMTLAGVMHFVKPQWYERIMPPYLPAHRELVYA
                     SGVAEIAGALGTMHPRTRRPAGLFLVATLAAVFPANVHMAINPDEFEAVPGGQAALYA
                     RLPLQALFVWWVWSATQR"
     gene            7161..8804
                     /locus_tag="VF517_04035"
     CDS             7161..8804
                     /locus_tag="VF517_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052138.1"
                     /translation="MVSALRKSLLAAFVAACALPAATPAAEPASRYNANGQLHALVCV
                     PLALPEADLRFSDGSPTLFKVTNQLTYNDGRCPPNTVRLDYHETFPTSAGPLVFHRGG
                     NGYTDEPNTKYGQIRTADLATQPGEPVPSGGGRGAPCERLTAEPAYSVDVRSMPEEMH
                     YKRPQDLPNGSNTGSSYEHYGDPGADQGDRTDVHYTYLLWSFVNVRGGGIVRTLLAPR
                     QTVRTCDVEPVTMDSWDRDGNVNGSVTARYVQALSGTCRVYGWMVWSHRYAPDGPVPV
                     THAQPAPSAPADPAADPACPVAAGAAPPTVATGDAAGQPDGSATIGGTAHPEGTPTSY
                     RFEYGTGPELGQSTEPRNLSNAMRTNAVSAQLAGLALATTYHYRIVATGEHGTSAGEV
                     RTFRMPDPPPPPPPPPPPPPPPPPPEPVVLSGLQVSPAAVRRSRNRLGSSASIRYALT
                     KPATVTLTFSRATTGMLVGTTCRTAPRRGIPRGRRRCTLWKIAPGVMTQLAPAGPAKL
                     RFGGWVGRRPLAAGRYRVTAQPADGGPPALARVAGFRIR"
     gene            8863..10056
                     /locus_tag="VF517_04040"
     CDS             8863..10056
                     /locus_tag="VF517_04040"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX8052139.1"
                     /translation="MTTTPAHRRPRLSAVLREEERVTPLELFFDLVFVLALTQCTALM
                     AAVPSWEGLAQALLIMALLWWSWSGYAWLTSVLDPEEGAVRIVMFAAMAAFLVCALCV
                     PTAFDDNALLFACAYGAVRLAHIGLFALASRDDPALRQSVIALGMSTAVSVGLLAGAS
                     ATDDVLQGAIWAVAIVLDFGGPYFFGISGWRIMPEHFAERFGLIVIIALGESIVAIGV
                     GAEVGVDAGVVAAAVLGTAGAAALWWLYFDVVVIVATRHLAKAAPGSEQNAIGRDSYG
                     YLHFPMIAGIVMGAMGLKKTIGDVGDSLELVPAVALFGGAAVYLLAHVAFRLRNIGTL
                     NRQRLGCAVLLCALIPLAKEVPSLASLGILVGLLSALIAYEAIRFSGARDRIRHQLAR
                     EAAAD"
     gene            complement(10270..11457)
                     /locus_tag="VF517_04045"
     CDS             complement(10270..11457)
                     /locus_tag="VF517_04045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX8052140.1"
                     /translation="MKAVAWHGKRDVRVDNVEDPTIQEPTDAIVRITTTAICGSDLHL
                     YEFMGAFLDEGDILGHEPMGIVEAVGSAVTGIAPGDRVVIPFNVSCGHCWMCERNLQS
                     QCETTQNHEWEKGASLLGYTKLYGQVPGGQAEYLRVPHADYGPVKVPHGPSDDRFVYL
                     SDVLPTAWQAVAYADVPDDGTLGVLGLGPIGDMCGRIGLHQGKRVIAFDLVPERIERA
                     KAQGMEAYNLEELDDPTAAVLDMTGGRGPDSVIDAVGMEAHGSTAANFALQMTTMLPD
                     AIVRPIMQRMGTDRMSALLLAIDMVRRGGTISLSGVYGGETDPMPMFTLFDKQVQLRM
                     GQANVRAWTDDILPLLTGDDDPLGVEDFATHRLPLDDAPDAYERFQKKDDGMVKVLLK
                     PEG"
     gene            11565..12071
                     /locus_tag="VF517_04050"
     CDS             11565..12071
                     /locus_tag="VF517_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052141.1"
                     /translation="MGSVTGMRLVVYGMVLVLAAIVLALRGGDDRAAAGTAAMYHGQT
                     PEKLLAAAVVRDGRVRGVYMRWRMTCERDRTPEISTIRFGEQFGDEFDYDGRRFSFAG
                     RSDQDVRRGLTTRYDVTLAGSVSADGRTVSGRGRTVETWLRGTRVVDRCHSSDVPFTA
                     HRGAVVSR"
     gene            complement(12061..12900)
                     /locus_tag="VF517_04055"
     CDS             complement(12061..12900)
                     /locus_tag="VF517_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEX8052142.1"
                     /translation="MVVDAPSLLFRAFYALPKSITDADDRPVNALLGTANMILFELER
                     HSPRAVVLCFGPDAATYRVEAFAGYHADRPEVPEELAHQFAGCAEFFGAFGWVIATHD
                     ELEADDLLGSYARAEVAAGGSALVMTGDRDMYQLAGEGITVLYVRTGGKQGAEVVDAA
                     EVKRRYGVPPELVPDFIALRGDPSDGIPGAKGIGEKTAADLLKRHGSLEAVLAAATRE
                     RPARVSGALIDGREQLPVFKHMATLQHVDVELPPDTPLDREGAAEAARARGMERLAKR
                     VSG"
     gene            complement(12912..13844)
                     /locus_tag="VF517_04060"
     CDS             complement(12912..13844)
                     /locus_tag="VF517_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8052143.1"
                     /translation="MRVFVAGATGAIGRPLVARLLEAGHEVIGMTRSDERAADLREGG
                     AEAVVCDAHDSEALRAAVTGARPEVLVHQLTDLPQEFNPRYDYGTTNELRSQVTRDLI
                     AAARDAGAGRFVAQGVAFFYVPTGSMVKDEEDPVTTSEVADGKFAEAGDALADLERQV
                     LGAEGMDGLVLRYGFFYGPGTWFAEDTKLAKAYRLRLAPIVGSGEGVFSFVHVDDAAA
                     ATVAAVERAAPGIYNVVDDEPAAANDWMPAFAEAVGGEPPLRVPAVVGRAAGGQNAEA
                     METMRGASNAKAKRELGWEPRYASWRQGFKEGLR"
     gene            complement(13841..14209)
                     /locus_tag="VF517_04065"
     CDS             complement(13841..14209)
                     /locus_tag="VF517_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052144.1"
                     /translation="MGLAAFALINGLTGLYAAVDPQGFYDNVGPFAPYNEHFIRDAAA
                     MQGTLGIAMAIAIWRRSWRVGVLGYGLLHFAFHAVNHLVDIGDADPESVGVVDFVGLA
                     IGALTLGWLFMRAVREDRAA"
     gene            complement(14295..15716)
                     /locus_tag="VF517_04070"
     CDS             complement(14295..15716)
                     /locus_tag="VF517_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX8052145.1"
                     /translation="MTKRLLIALSLIGCASLAGTVDADAATKKKARVNPAPAVKSIVP
                     MRAQVGEKLTINGTNFIAGKGKTKVYFLREGGGVAWVAADSGTRTRLIVTVPEKLIPL
                     LRGTAQNREATKFQVRILAKRFGSATKLAKSPIFTAPNAAPGDDGTPGTGTPAPGTPP
                     DGDCDGDGVKNSLDADDDNDALTDDLEVLPAHTDPCKPDTDGDTVADGYEYYSAKDIN
                     VTALPYPAKKPWPNPLDSEDAKIDHDGDGLDMVDEYLLWKYYGNLSVPLNYSAGLQKS
                     QDQDAPTQIELAYMDMDGDGKLSDDERDGDGDGLTNWDESHGRMLQDWWDAKYDGQNY
                     PSRKETRYPNTFPGVSIFDPDSDGDGLKDGADDQDHDGLTNAFENARPANWLSTYTWL
                     GYPDTFPYTNASDNPWSRVQPYNPCKPVWSYSCHLHPPFGHYESGEPWAGQMRSDAGP
                     QPTAPWTWPGSDPVSSLPSPPTG"
     gene            complement(15762..17480)
                     /locus_tag="VF517_04075"
     CDS             complement(15762..17480)
                     /locus_tag="VF517_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8052146.1"
                     /translation="MAEQVDVCIVGSGFGGSISAWRLAELYAAAGADPRSILVLERGR
                     RFKHTDFKQSMHIDHLASVYKLIQSSGGPGTIMASGPQGCQMVTANAVGGGSNLYLAA
                     SLRAPTEVFERRDRHPDDGPERRMWPAEISRRTLNGFYARAERALRVNQPGWDRVSKS
                     GGLWAATLHNAGHTCDRVPLAIDPGRCVEAKWCHTGCIFGAKNTVTTNYLASAERAGV
                     QVRPNRQVERIGRAGTAGYRYLVTAAQMDNEGEAPTRRPNGTNEEIECKVLILAAGAM
                     GTTPILMRSRAELPSLSGQLGRHLGSNGDHVAAIEYDPKRVRDVLGLPGYGAIHKGKP
                     ITTMTYDFYAGRRGSRYDGSRFTLQEIFLSSLTNFLYDDGREPAGDPSWWGLQKKQAI
                     SNWSNRIELLAMVEDTHDGEFVSSPPSGEALQPNAGPVEIGLFSYRPSEQSLRVRELA
                     DKAMRQIASRDGLGRFMQLTETQGVYCAHPLGGCRMAASSDLGVVDHRCEAFGNEGLF
                     CIDSSAIPTSLAVNPSLTIAAVAERAAAHLTARSHDLGLPRRPPGFKHRTPGVLVGER
                     VVPKKS"
     gene            complement(17473..18132)
                     /locus_tag="VF517_04080"
     CDS             complement(17473..18132)
                     /locus_tag="VF517_04080"
                     /inference="COORDINATES: protein motif:HMM:NF039951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5987 family protein"
                     /protein_id="HEX8052147.1"
                     /translation="MGEPRGPVARELSRRDLLARAGGLGLGAMTLAALPIAERMIAAD
                     PALAAVALDDATLQAFADTMIPGRPAERTDLGDEIHPQAIAGADPEPGAVEADALRLY
                     KNPLVGFAPALAGPFLSDLSVRSLRHGGAFLTLSFEQRTAVCIEALSFDNGDRVLYEA
                     AAAVPFTAFCAAAEHPIGTDENASGYRVMGYPGAAPDGYRSFSYHRKLANERTRRGSL
                     G"
     gene            18170..19210
                     /locus_tag="VF517_04085"
     CDS             18170..19210
                     /locus_tag="VF517_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052148.1"
                     /translation="MRSLVAIALAAGALAAAASPAAGQTGATGWDGANPFDCTLQQAG
                     SEAEVPQPDADPYCVEFDKRHQNVSEGGVVGFLALEPARVAAASPKCFYFQRDHWVGS
                     LQQGNSATQTYAWDGSYYFDKARGTGGAYVENFSFNGQSGDPSQLPGFPAEYRPFFGP
                     GRGGVQRSEAVEADPSCIAKAAEHPPYRQDRPGGPAGSEGMDRCRVAGGRVDRGIGGV
                     RLGVRRAGVRAKLGAPTIESARYASWCMTGGGTLVAAFDRSGERGRAVLVLTTAPPFD
                     ARGFRTGSKAYKARRGLHRERAIAKNVLAMKRRKDVLIAGLARGRVAYLASASRSLSA
                     KRTARFLAKAPR"
     gene            complement(19260..20240)
                     /locus_tag="VF517_04090"
     CDS             complement(19260..20240)
                     /locus_tag="VF517_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052149.1"
                     /translation="MAEPRAEARFPAVPRAKGHYESFYIRACHPTEPLGVWVRYTVHK
                     RPGAAPKGSLWFVLFDSAAGSPVASKLTTEDLSAGGGDYIRIGESSFRPGLAEGSART
                     EQADPRWRLALETREEPFPYLPAGWMYRAPVPRTKAESPYPDATFSGELDVDGRRIEV
                     DGWRGMVGHNWGTEHAERWIWTHGTLFEGGAWFDATLGRIKLGSVTTPWVANGALSID
                     GRRHRLGGPGRRTEVHERPDGAEFTISGKGVKVRGRVEAERKDLVGWVYADPDGPEHN
                     TVNCSIAQMTLTVERDGRPPVELTTPHGAAYELGMRETDHGIPLQPFPDG"
     gene            complement(20269..21288)
                     /locus_tag="VF517_04095"
     CDS             complement(20269..21288)
                     /locus_tag="VF517_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX8052150.1"
                     /translation="MRIALLALVLSLVAAAPAAAAVEIHAHRGGPVAAGVPVTPEDTQ
                     ESFAFGDSIGADVVELDAKLSSDRVPMVMHDATLDRTTDCDGQISQRTAAELAACHVD
                     VIGTEADIRQVPGATVAIPKLADVLAWAKANDVHLNLEIKNQPTDPDYDGSSGFATAV
                     LDAVTASGVPRDHVLIQSFWPPNLDVAKAAGFRTSFLTLAQSNNGSIEVAQSRGYDVM
                     SPAWPPSSNPTDYVQRAHAAGKPVVPYTFNKADEVQAAVEAGVDGVIANDVIVAQHVI
                     YGVDCPTSRTREASQRKSLARIRKARNRARGTNRIRLSAEVKRINGKRQAAKRLRLKV
                     CTPGA"
     gene            21320..22012
                     /locus_tag="VF517_04100"
     CDS             21320..22012
                     /locus_tag="VF517_04100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052151.1"
                     /translation="MADGPVLLITGASTGIGAATARQAVEAGYRVVLAARSEDKLRAL
                     AEELGGDEHGIAQRCDVAVWEDQEALVERALDAFGGIDAVFANAGFGAKRGFLEETPE
                     QWRSMVETNVLGCAYSIRSALPHFRERNAGHFVLTSSVAGRRALPGSLYSSTKWAVCG
                     MGESLRQELADTPIKVTLIEPGMVDTPFFDDRPQRALEDQDIARTVMFALSQPPHVDV
                     NEILVRPINQPT"
     gene            22028..23602
                     /locus_tag="VF517_04105"
     CDS             22028..23602
                     /locus_tag="VF517_04105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8052152.1"
                     /translation="MRGRIGGAAWLAIGTFAVATVINVVWALVDQDLPDYDAGRHLLY
                     AVSYRDAFATGDLLYPLTSWDPGQLGPLTYVVGALSMLLGGIDDDPAIIAADLIFGGA
                     LAFGCYGAGRRAFGSTAGALAVVFAFGAPLVVSQLHTFLADLPAAALVALAAWLLFDS
                     DRLTRLGPAAAAGVAIGLGVLTRPEVAVWLLGLIVVMLVRGAWRQWRGVLVLGVAALV
                     VGGPWYVLRWDDLRDRADSATRANPGSVGDVQGLIPDRFSGQNLGWYSWSTLNIELFL
                     PLFLLALGGAVWAAVRYVRNRDERDFTPELIGGYLTGYVLQTLVFTLKDPRYALPGLV
                     YLCVLGTGWIAAIGPPRRRMLAAAAVIAIAVLNVATIDLGIGGTLRATLPGAPDPPSL
                     PRHREIIVASNGRDIGLAATTPEDTGEPLLRVMEGARRDGARYVWFGGSDFPLFFAPA
                     GLTFFARAADLNFVRDGHPEFLGPKDVSLIKRAVEPGFPEPCVRLPDGTGIYVGRGQE
                     WRIPPSSYRPYCPIDD"
     gene            23652..24746
                     /gene="dinB"
                     /locus_tag="VF517_04110"
     CDS             23652..24746
                     /gene="dinB"
                     /locus_tag="VF517_04110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX8052153.1"
                     /translation="MTDVPRRCIAHVDMDAFYVSVELQRRPELRGKPVVVAGTGPRAV
                     VTTASYEARKFGVFSATPAERARRLCPQAIFVAPDFELYRARSREVMVVLHEHIDRVE
                     VVGLDEAYLDLSELERPKAAARRLKQAVRDRTGRNCSIGIGPNKLVAKVASDAEKPDG
                     FVVLSREEACVRFAGASPGLIPGIGPKTVQRLEAHGISTLGELAAIPGSALADWFGDR
                     LGPHLGALARFQDERAVQTVRVAKSESRETTFDTDLRGLDALEPVLERLAGELCQTLV
                     KQGASGRTIGIKVRLSDFSTHTRARTREQPTNDYATVSAVARELLREFDPPKPVRLIG
                     VRVAGLDEGERVRKQAVPAAAPAGQLEMTL"
     gene            24776..25048
                     /locus_tag="VF517_04115"
     CDS             24776..25048
                     /locus_tag="VF517_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052154.1"
                     /translation="MLATAVAFFWDDAEETVVEIVCLVALALLAVRLGGGRWFVVLVA
                     PLPAFVLLHERIPVDHWVAYGPAALSLAIAAWADGAWRTSSTTTAG"
     gene            complement(25032..25685)
                     /locus_tag="VF517_04120"
     CDS             complement(25032..25685)
                     /locus_tag="VF517_04120"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX8052155.1"
                     /translation="MSTYPRTRGRRYARALTAIALAGLATAASAPSTAAAADGITVNA
                     RHHVLAGEKVRISGRLGSGEAGRTVIVRVRHGGGWTTVDRVKTAAGGRYAAEWTPRGL
                     DRYGVRAGTLDGSVKRSLTDGITVYRRSAASWYGPGFYGSRTACGRTLAPGMVGVAHK
                     SLRCGTKVTLRYNGRTVVAPVIDRGPYAGNREYDLTAETKRRLGFGSTGTVWSSPQS"
     regulatory      complement(25689..25866)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(25941..27083)
                     /locus_tag="VF517_04125"
     CDS             complement(25941..27083)
                     /locus_tag="VF517_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8052156.1"
                     /translation="MYADQVLRPWLDAARALVPGVALFDAHTHTGSNDPDGFKCTHEE
                     LIAGLDVADARAVVFTTQEPDGYREANDRIIAEAEASGGKLVPFCRVDPAAEPLAEAE
                     RALGAGARGIKLHPRAERFRLADERLEPVFALAEERRVPVLTHAGRGIPALGRDALEI
                     CARHPDLPLILAHDGVSDLGWLWRHAAEHRNLYFDTSWWSTTDHLTLFGLVPPGQILF
                     ASDMPYGTSTVGAIMALRCALEVGLEAEQVRHIAGGQLERLLAGEDAADLGPAPGHQE
                     LRLDIVTQRIHDYLVAALSRMLIGDPAADYVGLARLACEVGEDAVHVDTARSVRELID
                     QIDPHVESTGYKPPEAGVPGPRLPYPAINIVVIAANVAMTPHVSVP"
     gene            27135..28607
                     /locus_tag="VF517_04130"
     CDS             27135..28607
                     /locus_tag="VF517_04130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8052157.1"
                     /translation="MAEDRLTGLDASFLHIESDSAHMHVAGVMLFQGSPPEHDELVEA
                     LERKMHLVPRYRQKLAFVPLQQGRPRWVDDPHFNLDYHVRLTALPAPGSEEQLRNLAG
                     RVFAQQLDRDKPLWEMWIVEGLDGGERFAVLSKTHHALVDGISGVDIASVLFDLSPDP
                     APVPDPPRRWLPRPLPSRGQLLAEALVERATVPAEVVRGVRAVFRAPRRIASAAVDAL
                     TGIGAMAWNGMSPAPPSPYNTSIGPHRRFTWARASLADVKAIKNSLGGTVNDVMLAVV
                     TGALRRDLARRGDLAAITELTAMVPVSVRSDDARGALGNQVAAMMAPLPVGHDDPVAR
                     LQTISAAMRRIKDSGQAMGAQMLTELTGFAPPTIMGQASRLVVRQRFFNLVVTNVPGP
                     QVPLYMMGREMIEIFPMVPLAPNQALGVAIMSYNGRINFGLVGDYDVMGNLDQVALDF
                     RASLEELAEAAGVELSARAATNGSSPSRRASLSAVPPPSA"
     gene            28732..29193
                     /locus_tag="VF517_04135"
     CDS             28732..29193
                     /locus_tag="VF517_04135"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX8052158.1"
                     /translation="MVERVTRAARELGLDIEVRTLDRPTRTVAEAAAAVGADPAQIAK
                     SLVFVSDGDPVLAVVSGAHRANIDVLADVFDCAEVRQASPDEVRGATGFSVGGVPPFG
                     HDLPVVFDEALCDQELIYAAGGDGNTLFAVDPKELARAIGAKIITLGGAPA"
     gene            complement(29190..31349)
                     /locus_tag="VF517_04140"
     CDS             complement(29190..31349)
                     /locus_tag="VF517_04140"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HEX8052159.1"
                     /translation="MTDLHPLVGRLTEAARVGRGGRLTWLGASVGVPAPAPETWVVAV
                     AGHLYGRFYVVGGPVPIAEAPVVGTEGHVFKGELARRTRTRRVPRELAVDHANGSLVH
                     ARRGELRIAVPRSALLDPPGGSPGTARVLVPGASFQDALGFLYLRGRRPEPEGAPDIV
                     RVYWNVTPAQALWLTRALPAVLDDADLAWEMKALDDPADYHRADSVVLYLPATAWPAA
                     RTAIAGVYARLPGAVAGPVPATTHELAPGLGLAEDPGDGRSFGEHLCTALATGLLAAG
                     PGADRNERASAMAGALQRAGMQPAAPHRRLGSDAAYSLTPARRRRARRRRPRDAGSAA
                     FEIGAELARRAIVWGDRATWLAAPLEEPGMGAGGASAETLDPFLYDGTAGMALFFAAL
                     ARADGDERWARLAHAAATQAITQVRPEGEPPGAGFHGGAGGLAWALVQTGRLLDSDEL
                     IESGVAVACAPRATTTTADEDLIAGLAGDLLAFALLERLAPGRVPAGRIDDAAARLDG
                     DKQPPKARSTGLAHGASGAALALAEAANTRGDDGLARSAAAWCEREAAWFDPERGNWA
                     DLRRTRSRAVAPAMTAWCHGAPGIGLARVCGGERLGPERAAEARSAADTTERWLRAAL
                     DRPQGDLGLCHGIAGNAWVLTRLDPGADALVEQAVQLVAHEVTAPRSAREVWTRPTRP
                     GLMIGWAGLGLFLLRAAGAADLPDPLAPAGWAGAPAG"
     gene            complement(31342..32373)
                     /locus_tag="VF517_04145"
     CDS             complement(31342..32373)
                     /locus_tag="VF517_04145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX8052160.1"
                     /translation="MAVSLGPDLGDGAAVLAWACRAGVLSPGQVVDERLTVTGFRGRN
                     DVFVVDSAAGRLVLKRFRQARDAAAEVRAHSRLERTSIAPHLLRLVARDGLLVVYESP
                     EGVVGLGRRLYGARPPNARLLRGMGRVLGGLHGCGAPPARTRRGLPWILGIADPAPVG
                     ILAQGVKLQRLVADLQARADVARALDRLAAAPGPRVPCHNDVKWENWLVAERGPLASR
                     LRLIDLEHAGPGPAAWDVGSVLANLCELWVDAVVERALGFREVRQLAAALAAGYAEAG
                     GTASSARCVAFAGARLLQTVYESVSYSAYVGEHAHRLLEMAAHLLTRPESAAVHLFGL
                     DARAFRPRD"
     gene            complement(32377..32877)
                     /locus_tag="VF517_04150"
     CDS             complement(32377..32877)
                     /locus_tag="VF517_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052161.1"
                     /translation="MADEDTLRLEGVLTGGDEGRLRLYVDDVLEEFIEFPEAGTIAIE
                     PLPREDDDFVQAVVVTLAAAAPTRRGRMDPAQAEHEARVAIEPEEQAAGGMRIQALGL
                     SRDKGDKSKISRGCTGPISDIICPSSKCTDGRLCHATEHCTNDAYCPVPTRVRRCDPD
                     TSTGPC"
     gene            complement(32902..34365)
                     /locus_tag="VF517_04155"
     CDS             complement(32902..34365)
                     /locus_tag="VF517_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8052162.1"
                     /translation="MAIDVQPQAPTHDAPPIERDQLYIGGEWVDPSGSGRFEVIDATT
                     EQVMGSVPAGDASDLDRAVAAARGAFEAWSAVSPYERADFCQAIAAKLAERSMEIAAL
                     VSREVGMPLHLSMQIQAGLPAMDFASMPAVVEDTVWEEQVGNSLIVREPVGVVGCITP
                     WNYPLHQICAKVAPALAAGCTVVVKPSEVTPLNAFILAEIMDELGLPAGVFNLVTGDG
                     PTVGEAMTRHPDVDMISFTGSTRAGKLVSQAASDTVKRVALELGGKSPNVILDDADLE
                     SAVVDGIGKCYLNSGQTCSALTRMLVPRAKLEAAEQIAAAAVSQIKVGDPFDPEVTLG
                     PLASEAQLERVRGYIRKGIDEGAKLVAGGEEAPEGASEGYFVRPTVFSEVDPKSTIAQ
                     EEIFGPVLSIIPYDSEDDAVRIANDTMYGLAGGVWSGDPEHAQAVARRIRTGQIEING
                     ATFNPLAPFGGFKQSGHGRELGKFGLEEFLEVKSLQL"
     gene            complement(34379..36382)
                     /locus_tag="VF517_04160"
     CDS             complement(34379..36382)
                     /locus_tag="VF517_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX8052163.1"
                     /translation="MEPQLSESQRDALRAFCDTIVPSIERAPDPDGFWGLSASDLAID
                     AAVADLVLGIPDETIRGGLLQLLDVLASQGIARPISQLSREQIIRNLQLASPEAAAGV
                     NALQGMTCFLHYGAPDPETGQNPNWKVFGYPGPTSAPPDVPKPLAPLDPEDGATLEAD
                     AVVVGSGSGGGVVAGTLAKAGLKVIVVEAGGYFNESDFAQLELKAYQEMYWRGGPNPT
                     ADGNISLQAGTTLGGGTVINWQNCLRTKPWVREDWARDYGLEGLDGPEYDRHLDTVLE
                     RISATDQASDLNGPHQRLKEGCEALGWGFETIVRNADRESYRPELAGYGGFGDVSGSK
                     QSADKTWLLDAAENGADILVRTRAQRVLVEGGRAAGVECVRLNDDGTPRGSVVVRAPR
                     VVVACGSLESPALLLRSGIGGPAVGQYLRLHPAEAVLGIYSEDQQGWWGAPQTGLCDE
                     FVDTGDGYGFLVEGAQYAPAIGASALPWTGGAEHKERMFDWKYGATIISLIRDRGYGR
                     VTIDDSGEAVPWYSVEDEVDLRNLRHALEAKIRINHAAGAKAIVSLAAGSPMWRYGDD
                     LEAFIQRARRMPMRAGGQKLFSAHQLGSCRMGSDPQTSVANPWGELHDTPGVWIGDGS
                     AFPTATGTNPMVSIMALAHRTAEAIAGKTDEPARGEPAAATAS"
     gene            36431..36607
                     /locus_tag="VF517_04165"
     CDS             36431..36607
                     /locus_tag="VF517_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052164.1"
                     /translation="MRRADDPRLQPIVWIVFAGTLILLMASGDSIGERLAMGAVFAVL
                     LAIYVLVILPRRRG"
     gene            complement(36624..37340)
                     /locus_tag="VF517_04170"
     CDS             complement(36624..37340)
                     /locus_tag="VF517_04170"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX8052165.1"
                     /translation="MRLLEAALNGEREHPAAPRTAAELAAAARACVDEGARAVHLHPW
                     SGGVQTLEAGPCAEALRAVREACPSALVSLSTSSAIEPDPERRRALVAGWTELPDLVS
                     ANQGEDGIVALCEDLLARGVGIEAGLLSVPDAEAFVESGLVERCAWALVEPLDAQPEA
                     AERHAAEIESVLAGAGVTLPQIHHGDGVASWAVSERGLARGHGIRTGLEDTTVLPDGR
                     PARDNAELVRAAAALMDGAA"
     gene            37476..37820
                     /locus_tag="VF517_04175"
     CDS             37476..37820
                     /locus_tag="VF517_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX8052166.1"
                     /translation="MIGIVRPEKANDVLEALYRAEVRGISIARVQGHGGELDRVETYR
                     GTTVQMGLADKVRFDIAVSDEFVDPTIDALCEGGRTGDVGDGKIFVVPLERAVRIRTG
                     ETDHGAVTPVGA"
     gene            37817..>38709
                     /locus_tag="VF517_04180"
     CDS             37817..>38709
                     /locus_tag="VF517_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX8052167.1"
                     /translation="MTLLAQAAKIDTGDTAWMLIATALVLLMTPALGLFYAGLVRGKN
                     TLNTFMMCVGAIAVATITWAAVGYSLAFDGTGDLVGGLHHAFLNDVTFEPRAGTTIPH
                     LLFFAFQATFCIITVALISGAVVERMRFGAYLVFAALWSVLVYSVLAHWGFGGGWLFE
                     GGTLDFAGGIAVEMASGFSALAAALVVGVRKDYARQALLPHNSMYVLLGAGLLWFGWF
                     GFNGGSGFTTGQNSVLAFTNTLLCPAATLVVWFALDLIRGRRVTAIGAATAIIVGCVG
                     ITPAAGFVSPVWAMVLGAIAAV"
BASE COUNT         5096 a        14152 c        13873 g         5588 t
ORIGIN      
        1 gctctcggcg ctgccgacgt cggcgatctt cttcaccagc gcgtagctcc cgaacgtgac
       61 cgcgaggatc agggcgatcc acggcggccg gccgtagtcg agcgtcagga ccagcaccgc
      121 cacggcgccc aggatcagcg ctgccgcctg ccacggacgc agccgctcgc ggaagacgag
      181 gatcccgaac aggacgctga ccagcgggtt gatgaagtag ccgagcgccg cctcgatcac
      241 gtgctcggag ttgaccgcgt agatgtagac gccccagttg accgccacca gcaccgcagc
      301 aagtgcgatc agccccgcgc ggcggcggtc gcgcagcagc gcgaggaacg gcctgaagcc
      361 ggcgccgagc gcgaggatca cgagcaccgc cacgagcgac cagacgatgc ggtggcccac
      421 gatctcgacc gggttcgcgg gctcgagcag cgggaagtac agcgggaaca gaccccagag
      481 caggtaggcg ccggtgccgt agacgatccc gcgcctgtgc tcggcctccc cgtggttcac
      541 ccgtttcact taagcagcgg cgaacgcccg gcggggccga tagcctgaga ccgcatggag
      601 gccacgcccc cgatcccgcc gccgcccccg ccgccgccga ccaccgcgct cgcgaacccc
      661 gcgctgcacg accacttcgt gctgcgccag cgctgggcgc cgttcgtgaa ccgctacgag
      721 ttctcggtgc cgggccacga ggacggggcg cccgagctgc ccgtgtgctt cgtgcagcag
      781 aagcggttca agttcaagga ggacatccgc ttctactcgg acgcctcgaa gaccgtcgag
      841 ctgatgcgga tcaaggcgcg ccagcggttc gacccgcggg cccgctacga catcaccgat
      901 ccgaacggct acaagatcgg tgagatccag aaggtattcg gcgagagcct gctgcgctcg
      961 acgttccggg tctacgacgc gaacggcgtc gagactgcga aggcgaccga gaagagcatg
     1021 gcggtcgccc tgatccgccg cttcatcggc ttcgtgccgt gggtgggctc gttcgccgat
     1081 tggctgccga tcccatacca cttcgtgttc ctgcgcggcg accacgtgct cgccacgaac
     1141 tcgcgccgcc tcttcaagat ccgcgacacc tacgacatcg acctgaccgg cgacccgcag
     1201 cggaccctgg accgccggct ggtgctggcg gcagtcgtgg ggatggacgc gctgcaggct
     1261 cgttagggcg catgacgacc gcggggtccc cgacgtcgac gatcgaagac gccgtggggg
     1321 aagccctgcg cgcgggcttc gtcgccggca ccgccctcgg ccccctcgcg ccgccgctcc
     1381 gcgaggagta catcgtgtcc gggctgctca ccagtggcgt gcccaccctc gacacggagc
     1441 tgcgccgcgt gctgcgcccg ttcggcttcg acctgacagt gggcggcgtg ttctgccatg
     1501 gcacacccaa ggtgcagctc aaaggaggcg gcagctgcga gctcgccgac ctcttggtcg
     1561 tagtgcgcta cgagaacggc gggttcgtcg agcgcaacgc cctgctcctc caagcgaaga
     1621 aagagggtgg tgctccgccg aagggcaacc agctccgcct gtacgaggat tggccggagt
     1681 tcgtctattc gagcgccgct ggactcaagg gcctcaagcg gagcgtgcac ccgaaggccc
     1741 cgggcccggg ggctcagttc gtcgaattgg ggacttgtcc gagctgcaag aagtgggcgg
     1801 ccggctgccc cgtggacggg tgggctgccg actcgtggcg accgggaaca gcgcggcgtc
     1861 cgttcctcaa cgagctcagt gacgtcatcc gcggatggag cggccgccgc ttcgagttgc
     1921 cgccgcgagc gcgcacgcgt ggatgggacc gggtgatcga ggatctgctg cgtgtgaccg
     1981 cggaacgcgc gttccgcgtg gccaagcgtg ccatggggtc cgcgggccga ggtgatgtcg
     2041 cccacgacta cgcccagatt ctccgtgagg agcgatgctg gccggttcag cctcggccgc
     2101 tggcgcttta ccgcgaccta ggtctcagcg aggaggccgc ggacccgtct gcgtggctca
     2161 gcgaggtccg agtcccgccg accctcccgc ccgacgcgct ggcgggaccc gtggacgacg
     2221 atgacggagc ggtctcactg ctgctggtcg agatcagccg cggcaaggac tcgagggccg
     2281 tcctttcgga cgagttcaca taagatccgt tatcgagcat cgatttgaga ttttccggcc
     2341 caaggccgga aggcgccaat tggtgggctc acccgccccc gacgcggatc gcgacgtcgc
     2401 cgattgtgat ctcgtcgccg gcgtggagct gccggccgcg gcgggactcc ggctcgccgt
     2461 tcacatgcac gtggccctcg gccagcagcg ccttggcgtc gccgccgccg ccgactatgc
     2521 ccgcgagctt caggagctgg ccgaggcgga tcatgtcgcc tcggatggcg acctcctgga
     2581 cgtcggtcat ccgccgccgt ggcagcagcc gcacgacggc gcgtccggcg cgtcgctctc
     2641 cgcgcgcagg atcgccgcct gccacttgcc cggaagatcc tcgagctcct gggccgcgac
     2701 cagcgccgcc gccagctcgt cgcgggcgga cgcgctgatg ccggcgagcg ggtcgcgccg
     2761 cagcgcttcg ctcacgcgtt ccgcgagggg gctcacggca tcagcgtacg cggagcccta
     2821 cacttaagcg agtgcttaag caacgcgtcc cgctggatct cacgttccag gcgctggccg
     2881 atcccacccg ccgcgacatg gtcgagcgcc tcagccgcgg cccggcatcc gtgagcgagc
     2941 tggccgagcc gctcaccatg tcgctgccgg cggtcgtgca gcacctccag gtgctcgagg
     3001 ccggcggcct ggtccggtcg gagaaggtgg gtcgcgtgcg cacctgtcac atcgaaccgg
     3061 acgccctcgg caccgcgcag cagtggatcg ccgaccggcg gacgcactgg gaacggcgcc
     3121 tcgaccgcct cggggacgtt ctcgccgacc aaccgaaggg agcggaatga acatcggaac
     3181 ccgcgacgaa tggctcgccg cccgcgtcga gctcctcgag cgcgagaagg aggtgacccg
     3241 cctgagcgac gaggtcgccg agcagcgccg cgccctgccg tgggtgccgg tcgagaagga
     3301 gtacgtcttc cacaccaacg acggcccgaa gaccctcgcc gagctcttcg acgggcgctc
     3361 gcagctcctc gtctaccact tcatgctcgg cccgaacacc cccgacggct gccccggctg
     3421 ctcgttcgga gccgaccact ggagtggtgc cgtcccccac ctcaacaacc acgacgtgac
     3481 gttcattctc gcctcgcgcg cagacctggc cacgatcaac gcctacaggg agcgcatggg
     3541 ctggaccatc ccgtgggtct cctccggagg gaccgacttc aacctcgact tcagcctgtt
     3601 caccgaggag cagcgtgcgt caggcacggg gttcaacttc ggcacccccc accgcgccaa
     3661 gcaccacttc gacgacatgg aggtcatggc gctgagcacg ttcgtcctag cggccggccg
     3721 cgtgcaccac acctacacga ccatggaccg cggcaccgac gtgctgatca ccacctggca
     3781 gttcctcgac cgcacgccgc gtgggcggtt cctcgtgccg agcgaggcgg aggccgccgg
     3841 tggctggccc aagcgccacg acgagtacgc gcagaccacg tgagcgtcga gcacgccacg
     3901 ttcgtcctcg agcgcgacta cccggcgccg ccggcgcggg tgttcaacgc gttcgccgac
     3961 ccggagctca agcagcgctg gttcggggct cccggctgga agtgggagat ggactttcgc
     4021 gagggcggcc gcgaacacac cagcggcgaa ttcgaagacg gaaccgcgat caccttcgat
     4081 tccgtctact acgacatcgt cccggacgaa cggatcgtct atgcgtacga gatgcactcc
     4141 gacggcagac gcatctccgt ctcggtcgct acgttcgagc tcgggccgcg cggtgacggc
     4201 acccgcctga ggctcaccga gcagggggcc ttcctcgacg gcgcagatac gcccggcact
     4261 cgcgagggtg gaacggtgag cctcctcgat gcccttggcg aggtgctggc tagcgctgcg
     4321 tgagcccgaa cggcgtgccc tcgctgtcgc ggcacacggc ccagctctcg cccggatgga
     4381 tcacctcgcc gccgagctcg gcgaccgacg ccagcgccgc gtcgaggtcg tcgaccgcga
     4441 agtacgggag ggcgaagcgg tccccgggcc cgcggccgcg ctcgtggatc ccgagcgccg
     4501 gaccgtcgcc gtgtgtctgc cagcccgagc cctcggcgga atcgcgcggg tcgagctcga
     4561 ggctcaggag accgcgccag aagctcagcg cgcgctcggc gtcgtcggcg gggaactcga
     4621 tcaactccat gccgcgcact ctacgccccg ccctcggcgc ccggaccgag cagcagccgc
     4681 gtgccgtagg acggctcgcc ctccagcacg tggtgcgcgt tgaccggtcg cagcgtgtag
     4741 gtctcgcccg cgacgacgag cgttcgcccc tcgtcgcgca cctcctcgac caccgccggc
     4801 tcgaccgtgc cgaagcgcat cacctgcacg ggcatgccca cgtgcggcgg cgggcgcgac
     4861 gggcgggtca aggcgaaccc cgcgcctagc cccacttcgc cgagcgcgcg agcttctgcc
     4921 gctcgcccgc cggcagcacg accttgcgca gccgcagctt gtcgggcgtg acctccacgc
     4981 actcgtcggt gcggatgaac tcgagcgcct ggtcgagcga gagctggtgg tgcggcacca
     5041 ggcgcaccgt gtcgtcggag ccggcggcgc ggatgttggt ctgctttttc tcgcggacgg
     5101 cgttcacgtc gaggtcgtcg gcgcgcgcgt tctcgcccac caccatcccc tcgtagacct
     5161 cctcgccagg ccccacgaag agctgcccgc gctcctgcat cttcagcagc gcgaactggc
     5221 ccgtctcgcc ccgccggtcg gcgacgagcg atccggtcgg gcgcgtcttg atgtccccgt
     5281 accacgggtc gtagcgctcg aagaccgaat ggaggatgcc gctaccacgg gtctcggtga
     5341 ggaactcggt ccggaagccg atcaggccgc gcgcgggcac gatgtactcg agccggaccc
     5401 agccggtgcc gtggttgacc atctgctcca tccggccctt gcgcagggcg agtagctgcg
     5461 tggtgacgcc gaggaagtcc tccggtacgt cgatcgccag gcgctcgact ggctcgtgca
     5521 gctttccgtc gatctcccgc gtgaccacct gcggcttgcc gacggtcagc tcgaagccct
     5581 cacgccgcat cgactcgacc agcaccgcga gctggagctc gccgcgcccc tgcacctccc
     5641 acgtgtcggg gcgctcggtg tcgagcacgc ggatcgagac gttgcccacc agctcggcgt
     5701 cgagccgctc gcgcacctgg cgcgcggtga gccgcgagcc cgagcgcccg gcgagcggcg
     5761 aggtgttgat cccgatcgtc atgccgagcg ccggctcgtc cacgttgatg ggcggcagcg
     5821 gccgcgggtc gcccgggtcg gcgagcgtct cgccgatggt gatctccggg atgccggcga
     5881 ccgcgacgat ctcccccggg cccgcctcgg gggcgtcgac ccggccgagg ccctccgcca
     5941 cgtacatctc ccccaccacg gcgcgctcga ccgacccgtc gcgccggcac cacgccaccg
     6001 cctcgccgcg gcgcagcacg ccgttgtgga tccggcaaag cgcgaggcgc ccgacgtacg
     6061 gggaggcgtc taggttggtc acgagcgcct ggagcccgtg cccgtcctcg tactcggggg
     6121 ccggcacgtg ctcgaccagg atgtcgagca gcggccgcag gtcggtgccc tcctcccccg
     6181 gatcgagcga cgcccagccg gccctcgcgt tcgtgtaggc gatcgggaac tcgatctgcg
     6241 cctcgtcggc gtcgaggtcg aggaacagct cgtagacctc gtccacgacc tcgccgatgc
     6301 gcgcgtccgg gcggtcgacc ttgttgacaa cgagaatcac cggcaggcgc cgctctagcg
     6361 cctttcgcag cacgaagcgc gtctgcggga gcggcccctc ggaggcgtcc acgagcagga
     6421 gcacgccatc gaccatcgac aggccgcgct ccacctcgcc gccgaagtcg gcgtggccgg
     6481 gggtgtcgac gatgttcagc ttcacctcgc cgtagcgcac ggcggcgttc ttcgcgagga
     6541 tcgtgatccc gcgctcgcgc tcctggtcca tggagtccat gacccggtcg gagacggcct
     6601 cgtgggcgcc gaacacgccc gactggcgca gcatggcgtc cacgagcgtg gtcttgccgt
     6661 ggtcgacgtg cgcgatgatc gcgacgttgc ggatgtcggt gcgggcggcc ataacggggc
     6721 cgccccaaca tacgagggcg gtagggtccg cccggcatga gtcgcgccgc cacgatcttc
     6781 gccgggccgg tcatgacgct cgccggcgtc atgcatttcg tgaagccgca gtggtacgag
     6841 cgcatcatgc cgccgtacct gccggcgcac cgcgagctcg tctacgcgag cggcgtggcc
     6901 gagatcgccg gggcgctcgg aaccatgcac cccaggacgc ggaggcccgc gggcctgttc
     6961 ctggtcgcga cgctcgcggc ggtcttcccg gccaacgtgc acatggcgat caacccggat
     7021 gagttcgagg ccgtgccggg cggccaggcc gcgctctacg cgcggctgcc cctccaggcc
     7081 ctgttcgtgt ggtgggtctg gagcgcgacg cagcgctaac tgacgccgca gcaaaccgcg
     7141 cctaccgtgg tttctcaccg atggtgagcg ccctccggaa gtccctgctc gccgcgttcg
     7201 tggccgcctg cgcactgccc gcggcgacac ccgcggccga gcccgccagc cgctacaacg
     7261 ccaacgggca gctccacgcg ctcgtgtgcg tgccgctggc cctccccgag gcggacctgc
     7321 gcttcagcga cggctccccc acgctcttca aggtcaccaa ccagctcacc tacaacgacg
     7381 gccgctgccc gccgaacacg gtgcggctgg actaccacga gaccttcccc acctcggccg
     7441 ggccgctcgt gttccaccgc ggcggcaacg gctacacgga cgagccgaac accaagtacg
     7501 ggcagatccg gacggccgac ctagccaccc agcctggcga gccggtgccc tccggcggcg
     7561 gccgcggcgc cccgtgcgag cggctcaccg ccgagcccgc gtactccgtc gacgtgcgct
     7621 cgatgcccga ggagatgcac tacaagcgtc cgcaggacct gccgaacggg tcgaacacgg
     7681 gttcctccta cgagcactac ggagaccccg gcgccgacca gggcgaccgc accgacgtgc
     7741 actacaccta cctgctctgg agcttcgtga acgtgcgcgg cggcggcatc gtgcgcacgc
     7801 tgctcgcgcc gcggcagacc gtgcgcacct gcgacgtcga gccggtgacg atggactcct
     7861 gggaccgcga cggcaacgtg aacggcagcg tgaccgcgcg ctacgtgcag gcgctctccg
     7921 ggacctgccg cgtgtacggc tggatggtct ggtcgcaccg ctacgcgccc gacggcccgg
     7981 tgccggtcac gcacgcgcag ccggcgccct ccgcgccggc cgaccccgcc gccgacccgg
     8041 cctgcccggt agcggccggc gccgcgcctc ccaccgtcgc gaccggcgac gccgcgggcc
     8101 agcccgacgg cagcgccacc atcggcggca ccgcccaccc ggagggcacg ccgacctcgt
     8161 accgcttcga gtacggcacc ggcccggagc tcggccagag caccgagccg cgcaacctct
     8221 cgaacgcgat gcgcacgaac gccgtgagcg cgcagctcgc cggcctcgcg ctcgcgacga
     8281 cctaccacta ccggatcgtc gcgaccggcg agcacggcac gagcgcgggc gaggtgcgca
     8341 cgttcaggat gcccgacccg ccgccgccgc caccgccccc gccgcctcct cctcccccac
     8401 ccccgccgcc ggagccggtc gtcctgagcg gcctccaggt gagcccggcc gcggtcaggc
     8461 gctcgcgcaa ccggctgggg tcgagcgcga gcattcgcta cgcgctcacg aagccggcca
     8521 cggtcacgct cacgttcagc cgcgccacga cgggcatgct ggtcggcacg acgtgccgca
     8581 cggccccgcg tcgcgggatc ccgcgcgggc gcaggcgctg cacgctgtgg aagatcgcgc
     8641 cgggcgtcat gacccagctc gcgcccgccg ggccggccaa gcttcgcttc ggcggctggg
     8701 tcgggcggcg ccccctcgcc gccggccgct accgggtgac ggcgcagccg gcggacggcg
     8761 gtccgcccgc cctcgcgcgc gtcgcgggct tccgcatccg ctgatccaac ccggggagcg
     8821 cccctggcac gtctgcacgg gctgcttgag taggcgccgg acgtgacgac gacgcccgca
     8881 caccgccgcc cccgcctcag cgccgtgctg cgcgaggagg agcgggtcac gccgctcgag
     8941 ctcttcttcg acctcgtgtt cgtgctcgcg ctgacccagt gcaccgcgct gatggcagcg
     9001 gtgccgagct gggagggcct cgcgcaggcg ctgctgatca tggcgctcct gtggtggtcg
     9061 tggagcgggt acgcgtggct caccagcgtg ctcgaccccg aggagggcgc cgttcggatc
     9121 gtgatgttcg ccgcgatggc cgcgttcctc gtctgcgcgc tgtgcgtacc gacggccttt
     9181 gacgacaacg cgctcctgtt cgcctgcgcc tatggagcgg tgcgcctcgc gcacatcggg
     9241 ctgttcgcac tggcctcgcg cgacgatccg gcgctgcgcc agtcggtcat cgcactgggg
     9301 atgagcaccg cggtgagcgt gggcctgctc gccggcgcct ccgccaccga cgacgtgctg
     9361 cagggagcga tctgggcggt cgcgatcgtg ctcgacttcg gcggccccta cttcttcggc
     9421 atctccggct ggcgcatcat gcccgagcac ttcgccgagc gcttcgggct gatcgtgatc
     9481 atcgcgctgg gcgagtcgat cgtggcgatc ggcgtaggcg cggaggtcgg ggtcgacgcc
     9541 ggtgtggtcg cggccgccgt gctcgggacc gcgggcgccg cggcactgtg gtggctgtac
     9601 ttcgacgtgg tggtcatcgt cgcgacccgg cacctggcga aggcggcgcc cggcagcgag
     9661 cagaacgcca tcggacgcga ctcctacggc tacctccact tcccgatgat cgccgggatc
     9721 gtgatgggcg cgatggggct gaagaagacg atcggcgacg tcggcgactc gctggagctc
     9781 gtgcccgcgg tcgcgctgtt cggcggcgcg gccgtgtacc tcctcgcgca cgtggcgttc
     9841 cggctgcgca acatcggcac gctcaacagg cagcggctcg gctgcgcggt cctgctgtgc
     9901 gcgctgatac cgctcgcgaa ggaggtcccg tcgctcgcgt cgctggggat cctcgtgggg
     9961 ctcctgagcg ctctgatcgc ctacgaggcg atccgcttct cgggtgcgag ggaccggatc
    10021 cggcaccagc tggcgcggga ggccgcggcg gactgacgcc cgcatcaggg caaaaaaagt
    10081 cccgccgcgg cgaggccggt ccgtgaaggc cggcctcgcc gacggcgggt gatccggggg
    10141 gcgcgggatc ccgacgccgt cggggttgtc ccgaacctca gcccgaggtt ctgcttcatg
    10201 tgtgttcgac gcttgtctcg atagagcagc ggcgtctaac gacgttgtcg gcgcggggtg
    10261 cgcggacctt tagccctccg gcttgagcag gaccttcacc atcccgtcgt ccttcttctg
    10321 gaagcgctcg taggcgtcgg gcgcgtcgtc gagcgggagc cggtgggtgg cgaagtcctc
    10381 caccccgagt gggtcgtcgt cgccggtgag cagcggcagg atgtcgtcgg tccaggcgcg
    10441 cacgttggcc tggcccatgc ggagctggac ctgcttgtcg aacagcgtga acatcggcat
    10501 cgggtcggtc tcgccgccgt agacgccgct cagcgagatg gtgccgccgc ggcggaccat
    10561 gtcgatcgcc agcagcagcg cgctcatccg gtcggtgccc atccgctgca tgatcggccg
    10621 cacgatcgcg tcggggagca tcgtggtcat ctggagggcg aagttcgcgg cggtggaacc
    10681 gtgcgcctcc atgccgaccg cgtcgatcac cgagtcgggg ccgcggccgc cggtcatatc
    10741 gagcacggcc gcggtggggt cgtcgagctc ctcgaggttg tacgcctcca tcccctgcgc
    10801 cttcgcgcgc tcgatccgct ccggcacgag gtcgaacgcg atcacgcgct tgccctggtg
    10861 gagcccgatc cgcccgcaca tgtccccgat cggaccgagg ccgaggacgc cgagggtgcc
    10921 gtcgtcgggc acgtccgcgt acgccaccgc ctgccaggcc gtggggagca cgtcggacag
    10981 gtagacgaag cggtcgtcgg agggcccgtg cgggaccttc accgggccgt agtcggcgtg
    11041 cggcacgcgc aggtactcgg cctgcccgcc cggcacctgc ccgtagagct tggtgtagcc
    11101 gagcagcgag gcgcccttct cccactcgtg gttctgcgtg gtctcgcact gcgactggag
    11161 gttgcgctcg cacatccagc agtggccgca ggagacgttg aacgggatca cgacgcggtc
    11221 gcccggcgcg atgccggtca ccgcgctgcc gaccgcctcg acgatgccca tcggctcgtg
    11281 gccgaggatg tcgccctcgt cgaggaaggc gcccatgaac tcgtagaggt gcaggtcgga
    11341 gccgcagatc gcggtggtgg tgatccgcac gatcgcatcg gtcggctcct ggatcgtcgg
    11401 atcctcgacg ttgtcgactc gtacgtcgcg cttgccatgc caggcgactg ccttcatgcg
    11461 ggtcctctcc tccagacggg ttcccggttc gcgtacccgc gggttgccgc gcgaaacgcc
    11521 tctgggcgct caggcgcggg ccgcggcatg ccgattccga accggtggga tcggtcaccg
    11581 gaatgcggct cgtggtctac ggcatggtcc tcgtgctggc ggcgatcgtc ctcgcgctgc
    11641 gcggcggcga cgaccgcgcg gccgctggca ccgccgcgat gtaccacggg cagacgccgg
    11701 agaagctgct ggccgcggcc gtggtgcgcg acgggcgggt ccgcggcgtc tacatgcgct
    11761 ggcgcatgac ctgcgagcgc gaccgcaccc cggagatctc gacgatccgc ttcggtgagc
    11821 agttcggcga cgagttcgac tacgacgggc ggcggttcag cttcgccggg cgctccgacc
    11881 aagacgtgcg ccgcgggctg accacgcgct acgacgtgac gctggccggg agcgtgtcgg
    11941 cggacgggcg aaccgtgagc ggccgcgggc gcaccgtcga gacctggctg cgcggtacgc
    12001 gtgtcgtgga ccgctgtcac tcgtctgacg tgcccttcac cgcgcaccgc ggcgcggtcg
    12061 tcagccgctg acgcgcttcg ccagccgctc catgccgcgc gcgcgggcgg cctcggcggc
    12121 tccctcgcgg tcgagcggcg tgtccggcgg gagctcgacg tccacgtgct ggagcgtggc
    12181 catgtgcttg aagaccggga gctgctcgcg gccgtcgatc agcgcgccgg agacgcgcgc
    12241 cgggcgctcg cgcgtggccg ccgcgagcac ggcctcgagc gagccgtggc gcttcagcag
    12301 gtcggccgcg gtcttctccc ctatcccctt ggctccgggg atcccgtcgg acgggtcgcc
    12361 gcgcaacgcg atgaagtcgg gcacgagctc gggcggcacg ccgtagcggc gcttgacctc
    12421 cgcggcgtcc accacctcgg ctccctgctt gccgccggtg cgtacgtaca ggacggtgat
    12481 gccctccccg gcgagctggt acatgtcgcg gtcgccggtc atgaccagcg cgctgccgcc
    12541 ggcggcgacc tcggcacgcg cgtaggagcc gagcaggtcg tcggcctcga gctcgtcgtg
    12601 ggtggcgatc acccagccga acgccccgaa gaactccgcg cagcccgcga actggtgcgc
    12661 cagctcctcc gggacctcgg gccggtccgc gtggtagccg gcgaacgcct cgacccggta
    12721 ggtggcggcg tcgggtccga agcagagcac gacggcgcgc ggcgaatggc gctcgagctc
    12781 gaacaggatc atgttcgcgg tgccgagcag cgcgttgacc ggccggtcgt cggcgtccgt
    12841 gatcgacttg ggcagcgcgt agaacgccct gaacaggagc gacggcgcgt cgaccaccat
    12901 gagcgggccg gctatcggag tccctccttg aatccctggc gccagctcgc gtagcgcggc
    12961 tcccagccga gctcgcgctt ggccttcgca ttgctggcgc cgcgcatggt ctccatggcc
    13021 tcggcgttct gaccacccgc ggcccgcccg accacggcgg gcacgcgcag cggcggctcg
    13081 ccgccgaccg cctccgcgaa cgccggcatc cagtcgttcg cggcggccgg ctcgtcgtcc
    13141 accacgttgt agatccccgg cgctgcgcgc tcgaccgcgg caaccgtggc cgccgccgcg
    13201 tcgtcgacgt ggacgaacga gaacactccc tcgcccgacc cgactatcgg cgcgaggcgc
    13261 agccggtacg ccttcgcgag cttcgtgtcc tcggcgaacc acgtccccgg cccgtagaag
    13321 aagccgtagc gcagcacgag gccgtccatg ccctcggccc cgagcacctg gcgctcgagg
    13381 tcggccagcg cgtcgccggc ctcggcgaac ttgccgtcgg ccacctcgga ggtcgtcacg
    13441 ggatcctcct cgtccttgac catcgagccg gtgggcacgt agaagaacgc tacgccctgc
    13501 gcgacgaacc gtcccgcgcc cgcgtcgcgc gccgcggcga tcaggtcgcg cgtgacctgt
    13561 gagcggagct cgttcgtcgt cccgtagtcg tagcgcggat tgaactcctg cgggaggtcg
    13621 gtgagctggt gcacgagcac ctccggccgt gccccggtga ccgccgcgcg cagcgcctcg
    13681 gagtcgtgcg cgtcgcagac cacggcctcg gcgcccccct cgcgcaggtc cgcggctcgc
    13741 tcgtccgagc gcgtcatgcc gatcacctcg tgacccgctt ccagcaggcg cgccacgagc
    13801 ggtcggccga tcgcgccggt cgcgcccgcg acgaagaccc tcaagccgcg cggtcctccc
    13861 gcacggcgcg catgaacagc cagccgagcg tgagggcgcc gatcgcaagt cccacgaagt
    13921 cgacaacgcc gacggactcg gggtcggcgt ccccgatgtc cacgaggtgg ttgacggcgt
    13981 ggaacgcgaa gtgcagcagc ccgtagccga gcaccccgac ccgccaagag cggcgccaga
    14041 tcgcgatcgc catcgcgatc cccagcgtgc cctgcatcgc ggcggcgtcg cggatgaagt
    14101 gctcgttgta gggcgcgaac ggcccgacgt tgtcgtagaa cccctggggg tccaccgcgg
    14161 cgtagaggcc ggtgaggccg ttgatcagcg cgaaggccgc gagccccacg ccgacgaggt
    14221 cacgccgtgt gccggtcggt ccgatgggcg caagttaacg cgtgcgccgc ctgcctgcgt
    14281 gccgggaagg ccgctcagcc ggtcggcggg ctcggcagcg agctcaccgg gtccgacccg
    14341 ggccaggtcc acggggccgt gggctgcggg ccggcgtcgg agcgcatctg accggcccac
    14401 ggctcgcccg actcgtagtg gccgaagggc gggtgcaggt ggcagctgta cgaccagacc
    14461 ggcttgcagg ggttgtacgg ctgcacgcgg gaccaggggt tgtccgacgc gttggtgtag
    14521 gggaaggtgt cggggtaccc gagccacgtg taggtggaga gccagttcgc ggggcgggcg
    14581 ttctcgaagg cgttggtcag gccgtcgtgg tcctggtcgt cggcgccgtc cttgaggccg
    14641 tcgccgtcgc tgtccgggtc gaagatgctg acgccgggga aggtgttcgg gtagcgggtc
    14701 tccttgcgcg acgggtagtt ctggccgtcg tacttggcgt cccaccagtc ctggagcatc
    14761 cggccgtgcg actcgtccca gttggtgagg ccgtcgccgt cgccgtcgcg ctcgtcgtcg
    14821 gagagcttgc cgtcgccgtc catgtccatg taggcgagct cgatctgcgt cggcgcgtcc
    14881 tggtcctggc tcttctggag gccggcgctg tagttcagcg gcaccgagag gttcccgtag
    14941 tacttccaca ggaggtactc gtcgaccatg tcgaggccgt cgccgtcgtg gtcgatcttc
    15001 gcgtcctcgg agtcgagcgg gttcggccac ggcttcttgg ccgggtacgg gagcgccgtg
    15061 acgttgatgt ccttggccga gtagtactcg tagccgtcgg ccacggtgtc gccgtcggtg
    15121 tcgggcttgc acgggtccgt gtgggccggc agcacctcga ggtcgtcggt cagggcgtcg
    15181 ttgtcgtcgt cggcgtcgag gctgttcttg acgccgtcgc cgtcgcaatc gccatcgggc
    15241 ggggtgcccg gggcgggcgt gcccgtgccc ggggtgccgt catcgccggg cgctgcgttc
    15301 ggcgccgtga agatcgggct cttggcgagc ttggtcgcgc tgccgaagcg cttggcgagg
    15361 atccggacct ggaacttggt ggcctcgcgg ttctgggcgg tgccgcgcag cagcgggatc
    15421 agcttctccg ggaccgtgac gatcaggcgc gtcctggtgc cgctgtcggc cgcaacccag
    15481 gcgacgccgc cgccctcacg caggaagtag accttggtct tgcccttgcc cgcgatgaag
    15541 ttggtgccgt tgatcgtcag cttctcgccg acctgggctc gcatcgggac gatcgacttg
    15601 accgccggcg cggggttgac cctggccttc ttcttcgtgg cggcgtcggc gtcgacggtg
    15661 ccggcgaggg acgcgcagcc gatcagggaa agggcaatga ggagtcgctt ggtcacgatt
    15721 ccgggtatcg gcccggcccc gcgctccctt taggggtcgg ttcaggattt tttcgggacg
    15781 acgcgctcgc ccacgagcac gcccggcgtg cggtgcttga agccgggcgg gcggcgcggc
    15841 aggccgagat cgtgcgagcg ggccgtcagg tgcgccgccg cacgctcggc gaccgccgcg
    15901 atggtgagcg acgggttcac cgccagcgag gtcgggatcg cggaggagtc gatgcagaac
    15961 agcccctcgt ttccgaacgc ctcgcagcgg tggtcgacca cgccgaggtc gctcgacgcc
    16021 gccatccggc agcccccgag cgggtgcgcg cagtagacgc cctgcgtctc ggtgagctgc
    16081 atgaagcggc cgagaccgtc gcgcgacgcg atctggcgca tcgccttgtc ggccagctcg
    16141 cgcacccgca gcgactgctc cgacgggcgg tacgagaaca ggccaatctc caccgggccg
    16201 gcattcggct ggagcgcttc cccggacggc ggcgaggaga cgaactcgcc gtcgtgggtg
    16261 tcctccacca tcgccaggag ctcgatccgg ttgctccagt tggagatcgc ctgcttcttc
    16321 tggagtcccc accaggacgg gtccccggcg ggctcgcggc cgtcgtcgta gaggaagttc
    16381 gtgaggctgg agaggaagat ctcctggagc gtgaagcggc tcccgtcgta gcgcgagccg
    16441 cggcgaccgg cgtagaagtc gtaggtcatc gtcgtgatcg gcttgccctt gtggatggcg
    16501 ccgtagccgg gcaggcccag cacgtcgcgg acgcgcttcg ggtcgtactc gatcgcggcc
    16561 acgtggtccc cgttcgagcc gaggtggcgg ccgagctgcc cgctgagcga cgggagctcg
    16621 gcccgcgagc gcatcaggat cggcgtcgtg cccatggcgc ccgcggccag gatcagcacc
    16681 ttgcactcga tctcctcgtt cgtcccgttc gggcgacggg tcggagcctc gccctcgttg
    16741 tccatctgcg cggccgtgac caggtagcgg tagccggccg tgccggcgcg cccgatccgc
    16801 tccacctggc ggttggggcg cacctgcacc ccggcgcgct cggcggacgc gaggtagttc
    16861 gtggtcaccg tgttcttggc cccgaagatg cagcccgtgt ggcaccactt ggcctcgacg
    16921 cagcggccgg ggtcgatggc gagcggcacc cggtcgcagg tgtggccggc gttgtggaga
    16981 gtcgcggccc agaggccgcc ggacttggag acgcgatccc agccgggctg gttcacgcgc
    17041 agggcccgct ccgcgcgcgc gtagaagccg ttcagcgtcc ggcgcgagat ctcggccggc
    17101 cacatgcggc gctcggggcc gtcgtcgggg tggcggtcgc ggcgctcgaa gacctccgtc
    17161 ggcgctcgca gcgaggcggc caggtagagg ttcgagccgc ctcccaccgc gttcgcggtg
    17221 accatctggc agccctgcgg gccggacgcc atgatcgtgc cgggcccgcc gctcgactgg
    17281 atcagcttgt agacgctcgc gaggtggtcg atgtgcatcg actgcttgaa gtcggtgtgc
    17341 ttgaagcggc gcccgcgctc gagcacgagg atcgacctcg ggtcggcgcc cgcggccgcg
    17401 tagagctcgg ccagccgcca cgccgagatc gacccgccga agccggagcc gacgatgcag
    17461 acgtcgacct gctcagccaa ggctgccccg cctggtccgc tcgttggcga gcttgcggtg
    17521 gtagctgaac gagcggtagc cgtccggcgc ggcgcccgga tagcccatca cgcggtagcc
    17581 ggaggcgttc tcgtcggtgc cgatcgggtg ctccgctgcc gcgcagaacg ccgtgaacgg
    17641 cacggcggcg gcggcctcgt agagcacgcg gtcgccgttg tcgaaggaga gcgcctcgat
    17701 gcagacggcg gtgcgctgct cgaagctcag cgtgaggaac gccccgccgt ggcggagcga
    17761 gcggacgctc aggtcggaca ggaacgggcc cgcgagcgcc ggcgcgaagc cgacgagcgg
    17821 gttcttgtac aggcgcagcg cgtcggcctc gaccgcgccc ggctccgggt cggcgccggc
    17881 gatcgcctgc gggtggatct cgtcgcccag gtcggtgcgc tcggccgggc ggccggggat
    17941 catcgtgtcg gcgaacgcct ggagcgtggc gtcgtcgagc gcgacggcgg cgagcgccgg
    18001 gtcggcggcg atcatccgct ccgcgatcgg cagcgcggcc agcgtcatcg cgcccagccc
    18061 gaggccgccg gcccgcgcga gtaagtcgcg gcgggagagc tcgcgcgcga ctggcccccg
    18121 aggttccccc atgccgccaa cgtacctcag gcgcgcgtat gctcgcccca tgcgctcgct
    18181 cgtcgcgatc gcgctcgcgg cgggggccct ggcggccgcc gcgagtcccg cggccggcca
    18241 gaccggcgcc accggctggg acggtgcgaa cccgttcgac tgcacgctcc agcaggccgg
    18301 cagcgaggcg gaggtgccgc agccggacgc cgacccgtac tgcgtcgagt tcgacaagcg
    18361 ccaccagaac gtgtcggagg gcggcgtcgt gggcttcctc gccctcgagc cggcgcgcgt
    18421 ggccgcggcc agcccgaagt gcttctactt ccagcgcgac cactgggtcg gctcgcttca
    18481 gcagggcaac tccgccacgc agacctacgc ctgggacggg agctactact tcgacaaggc
    18541 gcgcggcacc ggcggcgcgt acgtggagaa cttctcgttc aacggccaga gcggcgatcc
    18601 gtcgcagctt cccgggttcc cggcggagta caggccgttc ttcgggccgg gccgcggcgg
    18661 cgtgcagcgc agcgaggcgg tcgaggccga cccgagctgc atcgcgaagg cggccgagca
    18721 tccgccgtac cggcaggacc ggccgggtgg gccggcgggc tccgagggga tggaccgctg
    18781 ccgcgtggcc ggcgggcggg tggatcgcgg gatcggcggc gtgcggctcg gagtgcggcg
    18841 ggccggcgtg cgcgcgaagc tcggcgcgcc gacgatcgag agcgcgcgct acgcgagctg
    18901 gtgcatgacg ggcggcggca ctctggtggc cgccttcgac cgcagcggcg agcgcggccg
    18961 ggccgtgctc gtgctcacca cggcgccgcc gttcgacgcg cgcggcttcc gcaccggctc
    19021 gaaggcgtac aaggcgcgcc gcgggctgca ccgcgagcgg gcgatcgcga agaacgtgct
    19081 ggcgatgaag cgccgcaagg acgtgctgat cgcggggctc gcgcgcgggc gcgtggcgta
    19141 cctggcgagc gcgtcgcggt cgctcagcgc gaagcgcacc gcgcgcttcc tggcgaaggc
    19201 gccgcgctag cgcacctggt cgatcctgtg cacgctatac gtgcattggg tcgaccagtt
    19261 cagccgtccg ggaacggctg gagcgggatg ccgtggtcgg tctcgcgcat cccgagctcg
    19321 taggcggcgc cgtgtggggt cgtcagctcg accggcggcc ggccgtcgcg ctcgacggtc
    19381 agcgtcatct gcgcgatcga gcagttcacc gtgttgtgct ccggcccgtc cggatcggcg
    19441 tagacccagc cgacgaggtc cttgcgctcg gcctccaccc gcccgcggac cttcacgccc
    19501 ttgccgctaa tggtgaattc cgcgccatcc ggccgctcgt gcacctcggt cctgcggccc
    19561 gggccgccga ggcggtggcg gcggccgtcg atcgacagcg ccccgttcgc cacccacggc
    19621 gtggtcaccg acccgagctt gatccggccg agcgtcgcgt cgaaccaggc gccgccctcg
    19681 aagagcgtgc cgtgcgtcca gatccagcgc tcggcgtgct cggttcccca gttgtggccc
    19741 accatcccgc gccagccgtc gacctcgatc cggcgcccgt cgacgtcgag ctcgccgctg
    19801 aaggtcgcgt ccgggtacgg gctctcggcc ttcgtgcgcg gtaccggcgc gcggtacatc
    19861 cagccggcgg gcaggtacgg gaagggctcc tcgcgcgtct cgagcgcgag ccgccagcgc
    19921 gggtcggcct gctcggtccg cgcgctgccc tcggccagac ccggccggaa cgagctctcc
    19981 ccgatgcgta tgtagtcgcc gccgcccgcg ctcaggtcct ccgtggtgag cttcgaggcg
    20041 accggcgagc cggccgcgga gtcgaatagc acgaaccaga gcgagccctt cggggccgcc
    20101 cccgggcgct tgtggaccgt gtaccggacc cacacgccga gcggctcggt cgggtggcag
    20161 gcgcggatgt agaagctctc gtagtgcccc ttcgcccgcg gcaccgccgg gaagcgagct
    20221 tcggcgcgcg gctcggccat cacgcccgca ggctacccgc gaacgccgct acgcgcctgg
    20281 cgtacagacc ttcagtcgca ggcgcttcgc ggcctggcgc ttgccgttga tgcgcttgac
    20341 ctccgcgctc aaccggatgc gattggtgcc tcgcgctcgg ttgcgggcct tgcggatgcg
    20401 cgcgaggctc ttgcgctggc tcgcctcgcg ggtgcgcgag gtcgggcagt ccacgccgta
    20461 gatcacgtgc tgggcgacga tgacgtcgtt cgcgatcacg ccgtcgaccc ccgcctcgac
    20521 cgccgcctgc acctcgtcgg ccttgttgaa ggtgtagggc acgaccggct tgccggcggc
    20581 gtgggcgcgc tggacgtagt ccgtggggtt cgaggacggc ggccacgccg gcgacatcac
    20641 gtcgtacccg cgtgactggg ccacctcgat cgagccgttg ttcgactgcg cgagtgtgag
    20701 gaacgaggtc cggaacccgg ccgccttcgc cacgtcgagg tttggcggcc agaagctctg
    20761 gatcaacacg tggtcccgcg ggacgccgga cgccgtgacc gcgtcgagca ccgccgttgc
    20821 gaagcctgag ctgccgtcgt agtcggggtc ggtcggctgg ttcttgatct cgaggttgag
    20881 gtgaacgtcg ttcgcctttg cccaggcgag cacgtcggcc agcttcggga tggccaccgt
    20941 cgcgccgggc acctgcctga tgtcggcctc ggtcccgatc acgtcgacgt ggcacgcggc
    21001 cagctcggcc gccgtgcgct gggagatctg accgtcgcag tcggtggtgc gatcgagggt
    21061 ggcgtcgtgc atgaccatcg ggacgcggtc ggagctgagc ttggcgtcga gctcgaccac
    21121 gtccgcgccg atcgagtcgc cgaacgcgaa ggactcctgg gtgtcctcgg gcgtcaccgg
    21181 cacgccggcg gccaccggcc cgccgcggtg cgcgtggatc tccacggcgg ccgcggcggg
    21241 cgcggcggcg acgaggctca ggacgagggc gagcagtgcg atgcgcatgg cctcattgtg
    21301 cactgataga aatgcagcgg tggcagacgg gccagttctc ctcataaccg gagcaagcac
    21361 cgggatcggc gccgccacgg cgcgccaggc cgtggaggcc ggctaccggg tggtgctcgc
    21421 ggcgcgctcg gaggacaagc tgcgggcgct cgccgaggag ctgggcgggg acgagcacgg
    21481 catcgcgcag cgctgcgacg tggccgtgtg ggaggaccag gaggcgcttg tcgagcgggc
    21541 gctcgacgcg ttcggcggca tcgacgcggt cttcgccaac gccgggttcg gcgccaagcg
    21601 cggcttcctc gaggagacgc cggagcagtg gcgctcgatg gtcgagacga acgtgctcgg
    21661 ctgcgcctac tcgatccgct cggccctccc ccacttccgc gagcgcaacg cgggccactt
    21721 cgtcctaacc agctcggtcg cgggccgccg cgcgctgccg gggtcgctct actcgtccac
    21781 gaagtgggcg gtctgcggga tgggcgagtc gctgcgccag gagctggcgg acacgccgat
    21841 caaggtcacg ctgatcgagc ccggcatggt cgacacgccg ttcttcgacg accggccgca
    21901 acgcgcgctg gaggaccagg acatcgcccg gacggtgatg ttcgcgctct cgcagccgcc
    21961 gcacgtggac gtgaacgaga tcctcgtgcg cccgatcaac cagccgacct agccccgcat
    22021 gcgacacatg cggggacgga tcggcggcgc ggcgtggctc gcgatcggga cgttcgccgt
    22081 cgccacggtc atcaacgtgg tctgggccct ggtggaccag gacctcccgg actacgacgc
    22141 cgggcgccac ctcctctacg cggtctccta ccgggacgcg ttcgccacgg gcgacctgct
    22201 ctacccgctg acgagctggg accctgggca gctcggaccg ctcacgtacg tggtgggcgc
    22261 gctgtcgatg ctgctcggcg gcatcgacga cgacccggcg atcatcgccg ccgacctgat
    22321 cttcggcggc gcgctggcgt tcgggtgcta cggggccggc aggcgcgcgt tcgggtcgac
    22381 cgcgggcgcc ctggccgtcg tgttcgcgtt cggggcgcca ctcgtcgtct cgcagctcca
    22441 caccttcctc gccgacctgc cggcggcggc gctggtggcg ctggccgcct ggctcctgtt
    22501 cgacagcgac cggctcacgc gcctcggtcc ggctgccgcc gcgggggtcg ccatcggcct
    22561 cggcgttctc acccgcccgg aggtcgccgt ctggctcctc gggttgatcg tggtgatgct
    22621 cgtccgtggc gcctggcggc agtggcgggg cgtgctcgtg ctcggggtcg ccgcgctcgt
    22681 cgtcggcggg ccctggtacg tgctccgctg ggacgacctg cgcgaccgcg cggactcggc
    22741 cacgcgcgcg aatccgggct cggtgggcga cgtgcaggga ctgatcccgg accgcttcag
    22801 cggccagaac ctgggttggt acagctggag caccctcaac atcgagctgt tcctgccgct
    22861 cttcctgctg gcgctcgggg gggccgtctg ggccgcggtg cgctacgtgc gcaaccgcga
    22921 cgagcgcgat ttcacgcccg agctgatcgg cggatacctc accggctacg tgctccagac
    22981 gctggtcttt acgctcaagg acccgcgcta cgcgctgccg gggctcgtgt acctgtgcgt
    23041 gctggggacc gggtggatcg cggcgatcgg ccccccgcgc cggcggatgc tggcggccgc
    23101 ggccgtcatc gcgatcgccg tgctgaacgt tgcgacgatc gacctcggga tcggcggcac
    23161 gctgcgggcc acgctcccgg gggcgccgga cccgccgtcg ctgccgcgcc accgggagat
    23221 catcgtcgcc tcgaacggcc gcgacatcgg cctggccgcg acgaccccgg aggacaccgg
    23281 cgagccgctg ctgcgggtga tggagggagc acgccgcgac ggcgcgcgct acgtctggtt
    23341 cggcgggagc gacttcccgc tgttcttcgc accggcgggg ctcacgttct tcgcgcgcgc
    23401 ggcggacctc aacttcgtcc gcgacgggca tccggagttc ctcgggccga aggacgtctc
    23461 gctgatcaag cgcgcggtcg agccgggctt cccggagccg tgcgtccggc tgcccgacgg
    23521 gaccgggatc tacgtggggc gcgggcagga gtggcggatc ccgccgtcca gctacaggcc
    23581 gtactgcccg atcgacgact gacagcccga gcgcccgggt cgagccgcac gcgcgcgtac
    23641 gctggaggcg gatgaccgac gtgccgcggc gctgcatcgc tcatgtggac atggacgcct
    23701 tctacgtcag cgtcgagctc cagcgccgcc ccgagctgcg cggcaagccg gtagtcgtcg
    23761 cgggcacggg cccgcgcgcg gtcgtcacca ccgccagcta cgaggcgcgg aagttcggcg
    23821 tcttctcggc caccccggcc gagcgcgcgc ggcggctgtg cccgcaggcg atcttcgtcg
    23881 cgcccgactt cgagctctac cgcgctcgct cgcgcgaggt catggtcgtc ctgcacgagc
    23941 acatcgaccg ggtcgaggtg gtcgggctcg acgaggcgta cctcgacctc agcgagctcg
    24001 agcggccgaa ggccgccgcg cgccgcctca agcaggccgt gcgcgatcgg accgggcgga
    24061 actgctcgat cggcatcggg ccgaacaagc tcgtggcgaa ggtggcatcc gacgccgaga
    24121 agcccgacgg cttcgtggtc ctctcgcgtg aggaggcgtg cgtgcgcttc gccggcgcgt
    24181 ccccgggcct gatcccgggc atcggcccga agaccgtcca gcggctcgag gcccacggca
    24241 tcagcacgct gggcgagctg gccgcgatcc cgggcagcgc gctggccgac tggttcggcg
    24301 accggctcgg cccccacctc ggcgcactgg cccggtttca ggacgagcgc gccgtgcaga
    24361 ccgtccgcgt ggccaagtcc gagtcgcgcg agacgacctt cgacaccgac ctgcgcggcc
    24421 tcgacgcgct cgagcctgtg ctggagcgcc tcgccggcga gctgtgccag acgctcgtca
    24481 agcagggcgc gagcggccgg accatcggca taaaagtccg cctgtccgac ttctcgaccc
    24541 acacgcgcgc ccgcacgcgc gagcagccga cgaacgacta cgcgaccgtg agcgccgtgg
    24601 cccgcgagct gctgcgcgag ttcgaccccc cgaagcccgt gcggctgatc ggagtgcgcg
    24661 tggcggggct cgacgagggc gagcgcgtgc gcaagcaggc ggtacccgcc gccgcgcccg
    24721 cgggccagct ggagatgacg ctctgagaag cctcccggcg ctgctggccg cctacgtgct
    24781 ggcgaccgcc gtggcgttct tctgggacga cgccgaggag acggtcgtcg agatcgtctg
    24841 cctggtggcg ctcgcgctcc ttgccgtccg gctgggcggc ggccgctggt tcgtcgtgct
    24901 ggtcgcgccg ctccccgcgt tcgtgctcct gcacgagcgg ataccggtgg accactgggt
    24961 cgcctacggc cccgccgccc tgtcgctcgc tatcgcggcg tgggcggacg gggcctggcg
    25021 gacgtccagt actacgactg cgggctagac cagaccgtgc cggtcgagcc gaagccgagc
    25081 cggcgcttgg tctcggcggt gaggtcgtac tcgcggttgc ccgcgtacgg gccgcggtcg
    25141 atcaccggag cgacgaccgt gcgcccgttg tagcggagcg tcaccttcgt gccgcagcgc
    25201 aggctcttgt gcgcgacgcc gaccatgccg ggggccagcg tgcgcccgca ggccgtccgg
    25261 ctgccgtaga agccgggccc gtaccacgag gcggcgctgc gccggtagac ggtgatcccg
    25321 tccgtgaggc tgcgcttgac gctgccgtcg agggtgccgg cgcgcacgcc gtagcggtcg
    25381 agacccctgg gggtccactc cgccgcgtag cggccgccgg cggcagtctt cacgcggtcg
    25441 acggtcgtcc agccgccgcc gtggcggacc cggacgatga ccgtccggcc ggcctcgccc
    25501 gagccgagac ggcccgagat gcggaccttc tcgccggcca gcacgtggtg gcgggcgttg
    25561 acggtgatgc cgtcggccgc cgcggcggtg ctcggggcgc tcgccgccgt ggcgaggccg
    25621 gcgagcgcga ttgctgtcag ggcacgggcg tatcgacgcc cgcgtgtgcg ggggtacgtg
    25681 ctcacgtctc ttcctttccg gccggcgcct gcggggttag ctgtcgggct cgcgacgcgc
    25741 ctggcgcgcc gctacggccg gatcaccggc cgattcgccc cgtcagcggc gttgggtgac
    25801 gccgctgcag gtgggtcccc cgcccgctgc cactcggtgt gggcagcggt tcggctagag
    25861 gccgatcgaa cgaacgttcg taacggtacg aggcgctgcg gccgactccg tgagtcggcg
    25921 cacagtgcaa cggatcgccc tcagggcacg gagacgtgcg gcgtcatcgc cacgttcgcc
    25981 gcgatcacga cgatgttgat cgccgggtac ggcagccgcg gcccgggcac gcccgcctcg
    26041 ggcggcttat agccggtgga ctccacgtga ggatcgatct ggtcgatcag ctcgcgcacc
    26101 gaccgcgcgg tgtccacgtg cacagcgtcc tcccccacct cgcacgccag tcgcgcgagg
    26161 ccgacgtagt ccgccgcggg gtcgccgatc agcatccgcg agagcgcagc gacgaggtag
    26221 tcgtggatgc gctgggtcac gatgtcgagc cgcagctcct ggtggccggg cgcggggccg
    26281 aggtccgccg cgtcctcccc agcgaggagc cgctcgagct gaccgccggc gatgtggcgc
    26341 acctgctcgg cctctagccc gacctcgagc gcgcagcgca gggccatgat cgcgccgacc
    26401 gtgctcgtgc cgtacggcat gtcgctcgcg aacaggatct gccccggcgg taccagcccg
    26461 aacagcgtga ggtggtcggt ggtgctccac caggaggtgt cgaagtagag gttgcggtgc
    26521 tcggcggcgt ggcgccagag ccagccaagg tcggacaccc cgtcgtgggc gaggatcagc
    26581 ggcaggtcgg ggtggcgggc gcagatctcg agagcgtcgc gccccagggc ggggatgccg
    26641 cggccggcgt gggtgagcac cggcacgcga cgctcctcgg ccagcgcgaa cactggctcg
    26701 agccgctcgt ccgccaggcg gaaccgctcc gcgcgcgggt ggagcttgat cccgcgcgcg
    26761 ccggcgccga gcgcgcgctc ggcctcggcc agcggctcgg cggcgggatc gacgcggcag
    26821 aacgggacca gcttgccgcc ggaggcctcg gcctccgcga tgatccggtc gttcgcctcg
    26881 cggtagccgt ccggctcctg cgtcgtgaag accaccgcac gcgcgtccgc gacgtcgagc
    26941 ccggcgatca gctcctcgtg cgtgcacttg aagccgtcgg ggtcgttgct gcccgtgtgc
    27001 gtgtgtgcat cgaacagtgc gactccggga acgagcgccc tagcggcgtc gagccagggc
    27061 cggagaactt ggtcggcgta cacgggccgc gtaatgtaag gcccggcgat cccgactttg
    27121 agaggagggg agagatggcg gaggacaggc tcactggact cgacgcgtcg ttccttcaca
    27181 tagagagcga cagcgcccac atgcacgtgg cgggcgtgat gctgttccag ggctcgcccc
    27241 cggagcacga cgaactggtc gaggcgctcg agcgcaagat gcatctcgtc ccccgctacc
    27301 gccagaagct ggcgttcgtg ccgctccagc aggggcgccc gcgctgggtc gacgatcccc
    27361 acttcaacct cgactaccac gtgcgcttga ccgcgctgcc cgcccccggc agcgaggagc
    27421 agcttcgcaa cctcgccggg cgcgtgttcg cgcagcagct cgaccgcgac aagccgctgt
    27481 gggagatgtg gatcgtcgag gggctcgacg gcggcgagcg cttcgccgtg ctgagcaaga
    27541 cccaccacgc gctggtcgac gggatctccg gcgtcgacat cgcgagcgtg ctcttcgacc
    27601 tctcccccga tcccgccccg gtcccggacc cgccgcggcg gtggctgccg cggccgctgc
    27661 cctcgcgcgg gcagctgctc gccgaggcgc tcgtggagcg cgcgacggtg ccggccgagg
    27721 tcgtccgcgg cgtgcgggcg gtgttccgcg cgccgcgccg gatcgccagc gcggccgtgg
    27781 atgccctgac ggggatcggc gcgatggcgt ggaacgggat gagtccggcg ccgccttcgc
    27841 cgtacaacac gtcgatcggc ccgcaccggc gcttcacctg ggcccgagcg tcgcttgccg
    27901 acgtcaaggc catcaagaac tcgctcggcg gaaccgtgaa cgacgtgatg ctggcggtcg
    27961 tgacgggcgc gctgcggcgc gacctcgccc ggcgcggcga cctcgccgcg atcaccgagc
    28021 tgaccgccat ggtgccggtg agcgtgcgta gcgacgacgc ccgcggcgcg ctcggcaacc
    28081 aggtcgcggc gatgatggcc ccgctgcccg tcgggcacga cgacccggtc gcccgcctcc
    28141 agacgatctc cgccgcgatg cggcggatca aggacagcgg ccaggcgatg ggagcgcaga
    28201 tgctcacgga gctgaccggc ttcgcgccgc cgacgatcat gggccaggcg tcgcggctcg
    28261 tggtgcgcca gcggttcttc aacctggtcg tgacgaacgt gccggggccg caggtgccgc
    28321 tctacatgat gggccgggag atgatcgaga tcttcccgat ggtcccgctg gcgccgaacc
    28381 aggcgctggg tgtggcgatc atgagctaca acggccggat caacttcggt ctggtcgggg
    28441 actacgacgt catggggaac ctggaccagg tggcgctgga cttccgcgcg tcgctggagg
    28501 agctggccga ggcggcgggc gtggagctga gcgcacgcgc ggccaccaac ggcagcagcc
    28561 cgtcccgccg cgcgagcctg agcgcggtgc ccccgccctc ggcgtagccc gaccgtcctg
    28621 ccctagaggg acatagtccc cctaatccag gacaaacgcc gccttttccc cacgagcggc
    28681 tgcgcgccgg gccggtggcg gtggggtggc gcccgtcgta ggctggaatc gatggtcgag
    28741 agggttacga gggcggcgcg cgagctcggg ctcgatatcg aggtgcgcac gctcgacagg
    28801 ccgacgcgga ccgtggccga ggccgccgcg gcggtgggag ccgatccggc gcagatcgcg
    28861 aagtcgctgg tgttcgtctc cgacggcgac ccggtgctcg cggtggtctc cggtgcgcac
    28921 cgcgcgaaca tcgacgtgct ggccgacgtg ttcgactgcg cagaggttcg tcaggcatcg
    28981 ccggacgagg ttcgcggcgc cacgggcttc tctgtgggcg gggtaccgcc cttcggccac
    29041 gacctgccgg tggtcttcga cgaagccctc tgcgaccagg agctgatcta cgcggccggc
    29101 ggggacggca acacgctctt cgcggtggac ccgaaggagc tggcccgggc gatcggcgcg
    29161 aagatcatca cgctgggcgg cgctccggcc tagccggctg gcgctccggc ccagccggcc
    29221 ggggccagcg ggtccggtag gtcggcggct cccgccgctc gcaggaggaa gaggccgagg
    29281 ccggcccatc cgatcatcag gcccggccgc gtggggcggg tccagacctc ccgggccgat
    29341 cgcggcgccg tgacctcgtg tgcgacgagc tgcaccgcct gctcgaccag cgcgtccgca
    29401 cccgggtcga ggcgtgtgag cacccatgcg ttgccggcga tcccgtggca gagcccgaga
    29461 tcgccctgag gacggtcgag ggcggcccgc agccagcgct ccgtcgtgtc ggccgcgctg
    29521 cgtgcctccg cggcccgctc gggcccaagc cgctcgcccc cacagacgcg cgccagcccg
    29581 atccccggtg cgccgtggca ccacgccgtc atcgccggcg ccacggcgcg cgagcgcgtg
    29641 cgccggaggt cggcccagtt gccgcgctcc ggatcgaacc aggcggcctc gcgctcgcac
    29701 caggcggccg ccgaacgagc cagcccgtcg tccccgcgcg tgttcgcggc ctccgcgagc
    29761 gcgagcgccg cgcctgacgc accgtgcgcc aggcccgtgc tccgcgcctt cggcggctgt
    29821 ttgtcgccgt cgagccgggc ggccgcgtcg tcgattcgac ccgccgggac gcggcccggc
    29881 gcgaggcgct ccagcagggc gaacgcgagg aggtcacccg cgaggcccgc gatgaggtcc
    29941 tcgtccgcgg tcgtggtcgt cgcgcgcggc gcgcacgcca ccgcgacccc gctctcgatc
    30001 aactcgtcgc tgtccagcag ccgcccggtc tgcaccagcg cccaggccag gccgccggca
    30061 ccgccgtgga acccagcgcc cggcggctcc ccctcgggcc ggacctgcgt gatcgcctgg
    30121 gtcgccgccg cgtgcgccag ccgcgcccag cgctcgtcgc cgtccgcacg cgcgagcgcc
    30181 gcgaagaaca gcgccatgcc ggccgtgccg tcgtagagga atgggtccag cgtctccgcc
    30241 gaggcgccgc ccgcgcccat gccgggctcc tcgagcgggg cggcgagcca cgtcgcccgg
    30301 tcgccccaga cgatcgcgcg cctcgcgagc tcggcgccga tctcgaacgc agcggacccc
    30361 gcgtcgcgag gacgccgccg gcgggcgcgc cgccgccgcg ccggcgtcag cgagtaggcg
    30421 gcgtcggagc cgagccggcg gtgcggtgcc gcgggctgca ttccggcgcg ctggagtgcc
    30481 ccggccatgg cgctggcgcg ctcgttccgg tccgcacccg gcccggccgc gagcagtccc
    30541 gtagccagcg ccgtgcagag gtgctccccg aagctcctcc cgtcacccgg gtcctcggcc
    30601 agcccgagac ccggagcgag ctcgtgcgtc gtcgcgggca ccggcccggc gacggcaccc
    30661 ggcaggcgcg cgtagacgcc ggcgatggcc gtgcgcgcgg cgggccatgc cgtggccggc
    30721 aggtagagca ccacgctgtc ggcgcggtgg tagtccgccg ggtcgtcgag ggccttcatc
    30781 tcccaggcca ggtcggcgtc gtcgagcacg gcaggcaggg cgcgcgtcag ccagagtgcc
    30841 tgggcggggg tgacgttcca gtacacgcgc acgatgtcgg gcgcgccctc cggctccggt
    30901 ctccggccgc gcaggtacag gaagcccagc gcgtcctgga acgaggctcc cgggaccagc
    30961 accctcgccg tccccggtga cccaccgggc gggtcgagaa gcgcggaccg cgggaccgcg
    31021 atccggagct cgccacggcg tgcatgcacc agcgagccgt tcgcgtggtc caccgccagc
    31081 tcgcgcggga cacggcgggt gcgggtccgg cgcgccagct cgcccttgaa cacgtggccc
    31141 tccgtgccca ccaccggggc ctcggcgatg ggcaccggac cgccaacgac gtagaagcgc
    31201 ccgtacaggt ggcccgccac cgccacgacc cacgtctccg gcgccggcgc cggcacaccg
    31261 acactcgcgc cgagccaggt cagccgcccg ccgcggccca cccgcgcggc ctcggtgagc
    31321 cgcccgacca gtggatggag atcagtcacg tggacggaac gcgcgggcat cgagtccgaa
    31381 caggtgcacc gccgccgatt ccgggcgggt cagcaggtgg gcggccatct ccaggagccg
    31441 gtgcgcgtgc tcgccgacgt atgcgctgta cgacaccgac tcgtacaccg tctgcagcag
    31501 gcgtgcgccc gcgaacgcga cgcagcgggc gctgctcgcg gtgccgccgg cctccgcgta
    31561 gccggcggcg agagcggcgg cgagctggcg cacctcccgg aagccgagcg cgcgctccac
    31621 gaccgcgtcc acccagagct cgcacaggtt ggcgagcacc gagcccacgt cccacgcagc
    31681 tggccccggg ccggcgtgct cgaggtcgat gagccgcagc cggctcgcga gtgggccgcg
    31741 ctccgcaacg agccagttct cccacttcac gtcgttgtgg cacgggaccc gtgggccggg
    31801 cgccgccgcc agccggtcga gggcgcgtgc aacgtcggcg cgcgcctgga ggtccgccac
    31861 gagccgctgc agcttcacgc cctgagcgag gatcccgacc ggcgccgggt ccgcgatccc
    31921 gagtatccat ggcaggccgc gccgcgtccg cgccggaggc gctccgcacc cgtgcagccc
    31981 gccgagcacg cggcccatgc cccgcaggag gcgcgcgttg gggggccggg ctccgtagag
    32041 ccggcggccc agaccgacca cgccctcggg gctctcgtac acgaccagga gaccgtcgcg
    32101 cgcgacgagg cgaagcaggt ggggagcgat cgacgtgcgc tcgagacgcg agtgcgcgcg
    32161 cacctcggcg gccgcgtcac gcgcctgccg gaagcgcttg agaaccaggc ggccggcggc
    32221 cgaatcgacg acgaagacgt cgttgcggcc cctgaagccg gtgaccgtca agcgctcgtc
    32281 gacgacctgc ccgggcgaga gcacgcccgc ccggcacgcc cacgcgagca ccgcagcccc
    32341 gtcgccgagg tcaggcccga ggctgaccgc caccggctag cacggacccg tactggtgtc
    32401 cggatcgcac cggcgaacgc gcgtcggcac cgggcagtac gcgtcgttgg tgcagtgctc
    32461 ggtggcgtgg cagagtctgc cgtcggtgca cttcgagctc gggcagatga tgtcgctgat
    32521 cgggccggtg catccccggc tgatcttgga cttgtcgccc ttgtcgcggc tcagacccag
    32581 cgcctgtatc cgcatcccgc ccgcggcctg ctcctcgggc tcgattgcca cacgcgcctc
    32641 gtgctcggcc tgtgccgggt ccatccggcc gcgccgggtg ggtgccgccg ccgcgagtgt
    32701 gaccacgacc gcctggacga aatcgtcgtc ctcgcgcggg agcggttcga tcgcgatcgt
    32761 gccggcctcg gggaactcga tgaactcctc gagaacgtca tcgacgtaga gccgcaggcg
    32821 accctcgtcg ccaccggtca gcacgccctc gaggcgcagc gtgtcttcgt cggccatccc
    32881 tcagcccgtt cggcgcgcag cctagagctg gagcgacttc acctcgagga actcctccag
    32941 cccgaacttc cccagctccc gcccgtgccc cgactgcttg aagccgccga acggcgccag
    33001 cgggttgaac gtcgccccgt tgatctcgat ctggccggtc cggatccggc gcgccacggc
    33061 ctgcgcgtgc tccgggtccc ctgaccagac gccgccggcc aggccgtaca tcgtgtcgtt
    33121 ggcgatgcgc acggcgtcgt cctcggagtc gtaggggatg atcgagagca ccggcccgaa
    33181 gatctcctcc tgcgcgatcg tggacttggg atcgacctcg ctgaacacgg tcggccgcac
    33241 gaagtagccc tccgaagcgc cctcgggggc ctcctccccg cccgccacga gcttcgctcc
    33301 ttcgtcgatg cccttcctga tgtagccccg cacgcgctcg agctgggcct ccgacgccag
    33361 cggacccagc gtcacctccg gatcgaacgg gtcccccacc ttgatctgcg agaccgccgc
    33421 ggccgcgatc tgctccgccg cctcgagctt cgcccgcggc accagcatcc gcgtcagcgc
    33481 cgagcaggtc tgcccggagt tcaggtagca cttgccgatc ccgtcgacaa cggcggactc
    33541 gaggtccgcg tcgtcgagga tcacgttcgg cgacttgccg ccgagctcca gcgccacgcg
    33601 cttcacggtg tccgaagccg cctgggagac cagcttcccc gcccgcgtcg agcccgtgaa
    33661 cgagatcatg tccacgtccg ggtgccgcgt catcgcctcg ccgacggtcg ggccgtcgcc
    33721 cgtgacgagg ttgaacacgc cggccggaag cccgagctcg tccatgatct cggcgaggat
    33781 gaacgcgttc agcggcgtca cctcgctcgg cttcaccacc acggtgcagc cagcggccag
    33841 cgccggcgcc accttcgcgc agatctggtg cagcgggtag ttccacggcg tgatgcaccc
    33901 cacgacgccg actggctcgc ggacgatcag cgagttgccg acctgctcct cccagaccgt
    33961 gtcctccacg acggcgggca tcgaggcgaa gtccatcgcc ggcagccccg cctggatctg
    34021 catcgagagg tgcagcggca tccccacctc gcgcgagacc agcgccgcga tctccatcga
    34081 ccgctccgcc agcttcgccg cgatcgcctg gcagaaatcg gcgcgctcgt agggcgacac
    34141 ggccgaccac gcctcgaacg ctccccgcgc cgcggccacc gcgcgatcga ggtcggaggc
    34201 gtcgcccgcg ggcacgctgc ccatcacctg ctcggtcgtc gcgtcgatca cctcgaagcg
    34261 gccggacccg ctcgggtcca cccactcgcc gcctatgtag agctggtcgc gctcgatcgg
    34321 cggagcgtcg tgcgtcgggg cctggggctg gacgtcgatt gccatggtgc agagcctcct
    34381 agctcgcggt ggccgccgcg ggctcgccgc gcgccggttc gtcggtcttg cccgcgatcg
    34441 cctccgccgt gcggtgggcg agcgccatga tcgagaccat cgggttcgtg ccggtggccg
    34501 tcgggaacgc gctgccgtcg ccgatccaga caccgggcgt gtcgtgcagc tcgccccacg
    34561 ggttcgccac ggaggtctgc ggatcggacc ccatccggca cgagccgagc tggtgggcgc
    34621 tgaacagctt ctgcccgccg gcccgcatcg gcatccgcct ggcgcgctgg atgaacgcct
    34681 cgagatcgtc gccgtagcgc cacatcgggc tgcccgccgc gagcgagacg atcgccttcg
    34741 ccccggcggc gtggttgatc cggatcttcg cctcgagcgc gtggcgcagg ttgcgcaggt
    34801 ccacctcgtc ctcgacggag taccacggca cggcctcgcc gctgtcgtcg atcgtcaccc
    34861 ggccgtagcc gcggtcgcgg atcagcgaga tgatcgtggc cccgtacttc cagtcgaaca
    34921 tgcgctcctt gtgctccgcg ccaccggtcc acgggagcgc cgaggcgccg atcgcgggcg
    34981 cgtactgcgc tccctccacg aggaagccgt agccgtcgcc ggtgtcgacg aactcgtcgc
    35041 agagccccgt ctgcggcgcc ccccaccagc cctgctggtc ctccgagtag atcccgagca
    35101 ccgcctcggc cggatgcagc cgcaggtact ggcccaccgc cggcccgccg atcccgctgc
    35161 gcagcagtag cgccggcgac tcgagcgatc cgcacgcgac cacgacccgc ggcgcgcgca
    35221 cgaccacgct cccacgcggc gtaccgtcgt cgttcagccg cacgcactcc acgcccgcgg
    35281 cgcgcccgcc ctccaccagc acccgctgcg cgcgcgtgcg cacgaggatg tcggcgccgt
    35341 tctcggcggc gtcgagcagc catgtcttgt cggcgctctg cttgctgccg gacacgtcgc
    35401 cgaacccgcc gtagccggcc agctcgggcc tgtagctctc gcggtcggcg ttgcgaacga
    35461 tcgtctcgaa gccccagccg agcgcctcgc agccctcctt caggcgctgg tgcggaccgt
    35521 tcaggtcgct cgcctggtcg gtcgccgaga tccgctcgag caccgtgtcg aggtggcggt
    35581 cgtactccgg cccgtccagc ccctcgagcc cgtagtcgcg cgcccagtcc tcgcgtaccc
    35641 acggcttggt gcgcaggcag ttctgccagt tgatcacggt gccgccgccg agcgtggtgc
    35701 ccgcctggag cgagatgttg ccgtcggcgg tcgggttcgg tcccccgcgc cagtacatct
    35761 cctggtatgc cttcagctcg agctgcgcga agtcggactc gttgaaatag ccgccggcct
    35821 cgaccacgat caccttcagg cccgccttcg cgagcgtccc ggcgaccacg ccgccgcccg
    35881 agccggaccc cacgacgacg gcgtcggcct cgagcgtcgc cccgtcctcg gggtcgagcg
    35941 gtgcgagcgg cttcggcacg tccggcggcg cgctcgtcgg gcccgggtag ccgaagacct
    36001 tccagttcgg gttctgcccc gtctcggggt ccggcgctcc gtagtggagg aagcaggtca
    36061 tcccctggag cgcgttcacg cccgcggccg cctccggcga ggcgagctgg aggttgcgga
    36121 tgatctgctc gcgcgagagc tgcgagatcg gccgggcgat gccctgcgag gcgagcacgt
    36181 cgaggagttg gagcaggccg ccgcggatcg tctcgtcggg gatgcccagc acgaggtcgg
    36241 ccacggccgc gtcgatggcg agatcgctcg cgctgagacc ccaaaagccg tcgggatccg
    36301 gcgcgcgctc gatcgacggg acgatcgtgt cgcagaaggc gcgcagcgcg tcgcgctggc
    36361 tctcgctgag ctggggctcc atgacggtct ctcctcggtg cgtcgtgtgc cggtgggaga
    36421 ctaaaccccc gtgcgacgcg ccgacgaccc acgcctccag ccgatcgtct ggatcgtctt
    36481 cgcgggcacg ctcatcctcc tgatggcgag cggcgactcg atcggcgagc gcctcgccat
    36541 gggcgccgtc ttcgccgtgc tcctggcgat ctacgtgctc gtgatcctgc cgcggcggcg
    36601 gggctagggc gcgcttcggg gcgctaggcc gcgccgtcca tcagcgccgc ggccgcgcgg
    36661 accagctccg cgttgtcgcg cgcgggacgc ccgtccggca gcaccgtcgt gtcctcgagc
    36721 ccggtgcgga tgccgtgccc gcgcgcgagc ccacgctcgc tgactgccca gctcgccacg
    36781 ccgtcgccgt gatggatctg cggcagcgtc acgcccgccc cggcgagcac ggactcgatc
    36841 tccgcagcgt gccgctccgc cgcctccggc tgggcgtcga gtggctcgac cagcgcccac
    36901 gcgcagcgct cgaccagccc ggactccacg aacgcctcgg cgtcgggcac cgacaggagc
    36961 cccgcctcga tccccacccc acgcgcgagc aggtcctcgc acagcgcgac gatcccatcc
    37021 tccccctgat tggcgctcac caggtcgggc agctcggtcc accccgccac gagcgcgcgg
    37081 cggcgctcgg ggtccggctc gatggcggac gaggtgctga gcgacacgag cgcgctcggg
    37141 cacgcctccc gtaccgcccg cagcgcctcc gcgcacggtc ccgcctcgag cgtctgaacg
    37201 ccgcccgacc acggatgcag gtgcaccgcg cgggcaccct cgtccacgca cgcccgtgca
    37261 gcggcggcca gctcggccgc cgtgcgcggg gccgccgggt gctcgcgctc gccgttcagc
    37321 gctgcctcga gtagccgcaa ggcggcggat tcaaccaccc tcggccattc gtacaaccgc
    37381 gtgacgcgaa tcctgtacgg agggctaatg acgctcgggc gctgcgcgcc gacactcgcc
    37441 gccataagcg aaaaggagcg cacaacttga agctcgtgat cggcatcgtc cggcctgaga
    37501 aggccaacga cgtcctcgaa gccctctacc gcgccgaggt gcgcggcatc tccatcgccc
    37561 gcgtccaggg ccacggcggc gagctcgacc gggtcgagac ctaccgcggc acgaccgtcc
    37621 agatggggct cgcggacaag gtgcgcttcg acatcgcggt gtccgacgag ttcgtcgacc
    37681 ccaccatcga cgccctctgc gagggcggcc ggaccgggga cgtcggggac ggcaagatct
    37741 tcgtcgtgcc gctcgagcgg gccgtgcgca tccgcaccgg cgagaccgac cacggcgccg
    37801 tcaccccggt gggcgcatga ccctcctcgc tcaggcagcc aagatcgaca cgggcgacac
    37861 cgcctggatg ctcatcgcca ccgcgctcgt gctcctgatg acgccggcgc tcggcctctt
    37921 ctacgcgggc ctcgtccgcg gaaagaacac gctcaacacg ttcatgatgt gcgtcggcgc
    37981 gatcgccgtg gccaccatca cctgggcggc cgtcggctac tcgctcgcgt tcgacgggac
    38041 cggcgacctc gtcggcggcc tccaccacgc gttcctcaac gacgtcacgt tcgagccgcg
    38101 cgccggcacg acgatcccgc acctcctgtt cttcgcgttc caggccacgt tctgcatcat
    38161 cacggtggcg ctgatctccg gcgcggtggt ggagcggatg cgcttcggcg cgtacctcgt
    38221 gttcgctgcg ctgtggtcgg tgctcgtcta ctcggtgctc gcccactggg gcttcggcgg
    38281 cggctggctg ttcgagggcg ggacgctcga cttcgccggt ggcatcgccg tcgagatggc
    38341 gtccgggttc tcggcgctcg cggccgccct cgttgtcggc gtgcgcaagg actacgcgcg
    38401 ccaggcgctg ctgccgcaca actcgatgta cgtgctgctc ggggcgggcc tgctgtggtt
    38461 cggctggttc gggttcaacg gcggcagcgg cttcacgacc ggccagaaca gcgtcctcgc
    38521 gttcacgaac acgttgctgt gcccggccgc gacgctggtg gtctggttcg cgctcgacct
    38581 gatccggggg cgccgggtga cggccatcgg cgcggccacg gcgatcatcg tcggctgcgt
    38641 gggcatcacc ccggccgcgg gcttcgtcag cccggtgtgg gcgatggtgc tgggcgcgat
    38701 cgccgcggt
//
LOCUS       DASVPL010000089         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_50076, whole genome shotgun sequence.
ACCESSION   DASVPL010000089 DASVPL010000000
VERSION     DASVPL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50076"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VF517_04185"
     CDS             complement(<1..748)
                     /locus_tag="VF517_04185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8052168.1"
                     /translation="MARAHTLALLGIVLVGIAVRVAICDQSLFGDELFTSYVTEPDGL
                     GGVLDRVREIESNPPLFSVLAWGGAQLGDPEVWIRLPSMLAGIALIPLIGALGTLIGD
                     SRTGLTAAALVALSPFSTFFATEARAYATMTFLVALTTLALLCALSGRGRWWWAVFVV
                     AAVAALYTHYTTAFPLGVQAVWALWQHRGQARALLFTHGLAVLLFLPWIPLIRENEAE
                     QAIGGAFDLTPGSALSAMLRLVFGHAIESFD"
     gene            complement(824..1042)
                     /locus_tag="VF517_04190"
     CDS             complement(824..1042)
                     /locus_tag="VF517_04190"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX8052169.1"
                     /translation="MSDEALITPYRDGPLLVRGPFRMTDQDGNEIVVDRETVALCRCG
                     KSRMRPFCDGTHKLVRFRAPSAAEDGRA"
     gene            complement(1039..1449)
                     /locus_tag="VF517_04195"
     CDS             complement(1039..1449)
                     /locus_tag="VF517_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8052170.1"
                     /translation="MFTTSPPTADLSDAWIEGDPAARWRSSAGHGPGEGAKASGSSVL
                     EVDAGCRLPRHTDSAEETIVVVSGTASVTVGEEVAEVSAGGVALVPECVPHDVRNAGD
                     ETLRFVAVYAGTEVTTTYEDDVRPDGGRERDPIG"
     gene            1527..2969
                     /locus_tag="VF517_04200"
     CDS             1527..2969
                     /locus_tag="VF517_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX8052171.1"
                     /translation="MSSPSAQFSLTLRIEFDRRPGMLGSVATAIGDCGGSIGEIEMLE
                     FGDMKAVREITVDAVDREHGQAIVAAIQSIEGAELIDATDRTFELHRGGKLFTGLKTG
                     IRTRDDLSMAYTPGVARVCQAIEEDRDKAHELTIKSNTVAVVSDGTAVLGLGDIGPEA
                     AMPVMEGKAVLFKEFADVDAFPICLDTKDTDEIVQAVKLMAPGFGGINLEDISSPRCF
                     EIENRLIEELDIPVFHDDQHGTAIVVLAALLNACRLTDRRPEDLRVAMVGAGAAGVAV
                     AKIIMNEGVSEIVACDRNGSIHTDREDYVDGTMNTPKVWLADNTNGAVRAGSIQDALE
                     GADVFIGLSGPGIITAEDLQRMNPDPIVFAMANPNPEVRPEDAAKYARVIATGRSDYP
                     NQINNVLAFPGVFRGALDVRASAITEEMKVAAAHGIAAVVTDDELDEDHIVPSVFNRQ
                     VSHSVAEAVSREARQSGVARVGRDHAHIAY"
     gene            2988..3908
                     /locus_tag="VF517_04205"
     CDS             2988..3908
                     /locus_tag="VF517_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX8052172.1"
                     /translation="MRVAVTGATGLIGSALVRELLGRGDEVVALTRDPARAREQLGED
                     VSAVAWASPAAEPAPAHALSGCDGVVHLLGEPVNQRWSDEAKERIRNSRVFGTRYLVA
                     GLREAEPRPRVLVSQSAAGFYGPHGDEPVDETAPAAGDFLATVARDWEAEARVAEEEL
                     DMRVALMRTGVVLSESGGALGKMMTPFKLGVGGPVAGGRQYIPWIHMDDVVGGLVCAL
                     EDDRLRGPINLTAPEPATNRELSKALGRALHRPALAPAPGFAMKLMYGEMSSIVTTGV
                     RAVPARLAELGYRFRRPDLDDALRAAVGRD"
     gene            complement(3985..4707)
                     /locus_tag="VF517_04210"
     CDS             complement(3985..4707)
                     /locus_tag="VF517_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052173.1"
                     /translation="MPRERVDVSLPPAGKLAIAGGGLLLLVMVALLVAQLSVLADSRE
                     HIVAQDRKINRIVKGADPVLGELGPVADDARGAFRQATPFVRDLRATMVPLLRELRES
                     DLPGAIDVTARLVTSLERDGRLVRLVDQSNELIDALRASEFIPRTLRAADLAPEAFRI
                     ATEVLDVQRRTLHTQRRTLRVQRQTRAIQKETRAIQKQTLVIQQRLLAIQEEALVHIR
                     SIDRKTVAPPTDPAPLPAAPRR"
     gene            complement(4744..>4953)
                     /locus_tag="VF517_04215"
     CDS             complement(4744..>4953)
                     /locus_tag="VF517_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052174.1"
                     /translation="LGIVIWLLFPAKGDSKWKLVGPFPWSRGSGLTLAEARAQLAEGG
                     DGKTAAAKPAARDDGPPAEPPHVSP"
BASE COUNT          740 a         1794 c         1785 g          634 t
ORIGIN      
        1 cgtcgaacga ctcgatggcg tggccgaaga ccaaccgcag catcgcgctc agcgcgctgc
       61 ccggggtcag gtcgaacgcc ccgccgatcg cctgctcggc ctcgttctcg cggatcagcg
      121 ggatccaggg aaggaacagc agcaccgcga gcccgtgggt gaacagcagc gcgcgcgcct
      181 gaccgcggtg ctgccagagc gcccacacag cctgcacccc gagcgggaac gcggtcgtgt
      241 agtgcgtgta cagcgcggcg acggcggcga ccacgaacac cgcccaccac caccgcccgc
      301 gcccgctcag agcgcacagg agcgcgagcg tggtgagcgc cacgaggaac gtcatcgtcg
      361 cgtacgcgcg cgcctcggtc gcgaagaagg tggagaacgg cgagagcgcc accagcgccg
      421 ccgcggtcag gcccgtgcgc gagtcgccga tcagcgtgcc tagcgctccg atcagcggga
      481 tcagcgcgat cccggcgagc atcgagggaa gccggatcca tacctcgggg tcgccgagct
      541 gcgcgccgcc ccaggcgagc accgagaaca gcggcgggtt gctctcgatc tcgcggacgc
      601 ggtcgagcac gccgccgagg ccgtccggct cggtgacgta ggacgtgaac agctcgtccc
      661 cgaacaggct ctggtcgcag atggcgaccc gcactgcgat gcccacgagc acgatcccca
      721 gcagagcgag ggtgtgcgcg cgcgccatcg cgaggatcct atgtccggcg gaccggaggc
      781 cccaggcgcg gaggaagccc gcccgccccg gccacccctg ccgctaggcc cggccgtcct
      841 ccgccgcgct cggcgcgcgg aagcgcacga gcttgtgcgt gccgtcgcag aacgggcgca
      901 tccgcgactt gccgcagcgg cagagcgcca cggtctcgcg gtcgaccacg atctcgttgc
      961 cgtcctggtc ggtcatccgg aacggccccc gcacgagcag cggaccgtcg cggtacggcg
     1021 tgatcagcgc ctcgtcgctc atccgatcgg gtcgcgctcg cggccgccgt cgggccggac
     1081 gtcgtcctcg taggtggtgg tcacctcggt gccggcgtag acggcgacga agcgcagcgt
     1141 ctcgtcgccg gcgttgcgca cgtcgtgcgg cacgcactcg ggcacgagcg cgacgccccc
     1201 ggcggacacc tccgcgacct cctcgccgac cgtcaccgac gcggtgccgg acacgaccac
     1261 gatcgtctcc tccgcgctgt cggtgtggcg cggcagccgg cagcccgcgt cgacctcgag
     1321 caccgagctg cccgatgcct tggcaccctc gccggggcca tggccggcgg acgagcgcca
     1381 gcgggccgcg gggtcgccct cgatccaggc gtcgctcaaa tcggccgtcg gcggagaggt
     1441 cgtgaacatg cccggctgag atacccggac ccgctcgcgc gcaaccgatt tacccgcaaa
     1501 ggggcgctct atatgctcac ctgcccatgt cctccccgag cgcgcagttc tctctgaccc
     1561 tccgcatcga gttcgaccgc cgtcccggca tgctcggctc ggtcgcgacg gcgatcggcg
     1621 actgcggcgg ctcgatcggc gagatcgaga tgctcgagtt cggcgacatg aaggcggtgc
     1681 gcgagatcac ggtcgacgcg gtggaccgcg agcacgggca ggcgatcgtc gcggccatcc
     1741 agtcgatcga gggcgccgag ctgatcgacg ccaccgaccg aaccttcgag ctgcaccgcg
     1801 gcggcaagct cttcacgggc ctgaagaccg gcatccgcac gcgcgacgac ctctcgatgg
     1861 cctacacgcc cggcgtcgcg cgcgtctgcc aggcgatcga ggaggaccgc gacaaggccc
     1921 acgagctgac gatcaagagc aacaccgtcg ccgtggtctc cgacgggacg gccgtgctcg
     1981 gtctcggcga catcggcccc gaggccgcga tgcccgtgat ggagggcaag gcggtgctgt
     2041 tcaaggagtt cgccgacgtg gacgcgttcc cgatctgcct cgacaccaag gacacggacg
     2101 agatcgtcca ggccgtgaag ctgatggccc cgggcttcgg cggcatcaac ctcgaggaca
     2161 tctcctcgcc gcgctgcttc gagatcgaga accggctgat cgaggagctc gacatccctg
     2221 tgttccacga cgaccagcac ggcaccgcga tcgtcgtgct ggcggcgctg ctgaacgcct
     2281 gccggctgac cgaccgccgc cccgaggacc tgcgcgtggc gatggtcggc gccggtgctg
     2341 ccggtgtggc cgtcgcaaag atcatcatga acgagggtgt cagcgagatc gtggcctgcg
     2401 accgcaacgg gtcgatccac accgatcgcg aggactacgt cgacggcacg atgaacacgc
     2461 ccaaggtctg gctcgccgac aacaccaacg gcgcggtgcg cgcgggctcg atccaggacg
     2521 cgctcgaggg cgccgacgtc ttcatcggcc tctccgggcc cgggatcatc accgccgagg
     2581 acctccagcg gatgaacccc gacccgatcg tgttcgcgat ggcgaacccg aacccggagg
     2641 tgcggcccga ggacgccgcc aagtacgcgc gcgtcatcgc gaccggacgc tccgactacc
     2701 cgaaccagat caacaacgtg ctcgccttcc cgggcgtgtt ccgcggcgcc ctcgacgtgc
     2761 gcgccagcgc catcaccgag gagatgaagg tggcggccgc gcacggcatc gccgccgtcg
     2821 tgaccgacga cgagctcgac gaggaccaca tcgtccccag cgtgttcaac cgccaggtga
     2881 gccattcggt cgccgaggcc gtatcccgtg aggcgcgcca gagcggcgtc gcccgcgtgg
     2941 ggcgcgacca cgcgcacatc gcctactagg agaggaccgg gctgaccatg agggtcgccg
     3001 tcaccggagc gacaggcttg atcggctcgg ccctggtgcg cgagctgctg ggacgcggcg
     3061 acgaggtggt ggcgctcacg cgcgacccgg cgcgggcgcg cgagcagctc ggcgaggacg
     3121 tctcggcggt cgcctgggcg agccccgcgg ccgagccggc acccgcgcac gcgctctcgg
     3181 gctgcgacgg cgtcgtgcac ctcctcggcg agccggtcaa ccagcgctgg agcgacgagg
     3241 ccaaggagcg cattcgcaac tcgcgcgtgt tcggcacccg ctacctggtg gccggcctgc
     3301 gcgaggcgga gccgcgcccg cgcgtgctcg tgtcgcagtc ggccgccggt ttctacggcc
     3361 cgcacggcga cgagcccgtg gacgagacgg ctccggccgc cggcgacttc ctcgccacgg
     3421 tcgcgcggga ctgggaggcc gaggcgcggg tggccgagga ggagctcgac atgcgcgtgg
     3481 cgctcatgcg caccggcgtg gtgctgtcgg agtccggcgg cgcgctcggg aagatgatga
     3541 cgccgttcaa gctcggggtc ggcgggcccg tcgccggcgg gcggcagtac atcccctgga
     3601 tccacatgga cgacgtggtg ggcgggctcg tgtgcgcgct cgaggacgac cggctgcgcg
     3661 gccccatcaa cctcacggcg ccggagccgg ccacgaacag ggagctgtcg aaggcgctcg
     3721 gccgcgcgct gcaccggccc gcgctggcgc ccgcgccggg attcgcgatg aagctgatgt
     3781 acggggagat gtcgtcgatc gtgacgacgg gcgtccgcgc ggtcccggcc cggctggccg
     3841 agctgggcta ccgcttcagg cgcccggatc tggacgacgc actgcgcgcg gcggtcggcc
     3901 gcgactagcc atcgattgga gccttgtgta ccgcatagcg gagcaaccct ccaatcgatg
     3961 cgctcccgcg cgtccgcggg gtagctagcg ccgcggcgcg gccggcagcg gcgcggggtc
     4021 cgtcggcggc gcgaccgtct tgcggtcgat gctgcggatg tggaccagcg cctcctcctg
     4081 gatcgcgagg agccgctgct ggatcaccag ggtctgcttc tggatggcgc gcgtctcctt
     4141 ctggatcgcg cgggtctggc gctggaccct gagcgtgcgc cgctgggtgt gcagcgtgcg
     4201 ccgctgcacg tcgagcacct ccgtcgcgat ccggaacgcc tcgggcgcca ggtcggccgc
     4261 ccgcagagtg cgcgggatga actcgctcgc ccgcagggcg tcgatcagct cgttcgactg
     4321 gtccacgagc cgcacgaggc ggccgtcgcg ctcgagcgag gtgacgagcc gcgcggtcac
     4381 gtcgatggcg ccgggcaggt ccgactcgcg cagctcgcgc agcagtggga ccatcgtcgc
     4441 gcgcaggtcg cgcacgaacg gcgtggcctg ccggaacgcg ccgcgcgcgt cgtcggcgac
     4501 cggtccgagc tcgccgagca cgggatcggc gcccttcacg atccggttga tcttgcggtc
     4561 ctgcgcgacg atgtgctccc gcgagtcggc caggaccgag agctgcgcca cgagcagcgc
     4621 aaccatgacc aggagcagca ggccgccgcc cgcgatagcg agcttgccgg caggcggcag
     4681 ggacacgtcg acgcgctcgc ggggcaaacc gcaggacacc tacccccaaa cgggggacga
     4741 aaactaaggt gagacgtgcg gcggctcggc cggcgggccg tcgtcgcgag cggccggctt
     4801 ggccgccgcg gtcttgccgt cgccgccctc ggcgagctgc gcgcgcgcct cggcgagcgt
     4861 cagccccgac ccgcggctcc aggggaaggg gcccaccagc ttccacttcg agtccccctt
     4921 cgcggggaac agcagccaga tgacgatccc gag
//
LOCUS       DASVPL010000090         3445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_127614, whole genome shotgun sequence.
ACCESSION   DASVPL010000090 DASVPL010000000
VERSION     DASVPL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127614"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VF517_04220"
     CDS             <1..393
                     /locus_tag="VF517_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052175.1"
                     /translation="AARPAKSAAGSRAAAIAWSGSVATFAAALGFFGWQVAAGRDPAI
                     GAGPPDSQKPKKVIVRRIVKRTVIITTPAPSGGGGGGGGMPAGGAASGGGAAPSGGGA
                     PSGGGGGSAPAPAPAAAPAPAPPATSAS"
     gene            421..1389
                     /locus_tag="VF517_04225"
     CDS             421..1389
                     /locus_tag="VF517_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX8052176.1"
                     /translation="MAEHADITFTCMGCDMRVLVEDGPRSHAAARGARRQLEAIDAQL
                     SRFREDSELARLNADPRLVVPASPLLRAAVRAAIWAAEASGGLVDPTLLGDLEHAGYR
                     HSLTGIARVPLAEALKEAPPRAAARPRGDRRWRAIRVDDARGTIARPPGLRIDTGGTT
                     KGLAADAVAHVLGGTRHIVDCAGDLRLDAGAGDAFGVIVEHPLTRATADTVDVRGGAI
                     ATSGPGRRLWRDADGAIGHHLLDAATGAPAWTGLVSATALAPTALEAETLAKAALLGG
                     PTAARRVLARRGGVLIHDDGATERVGPIARPRLRLRTTERGLAVVL"
     gene            1386..1985
                     /locus_tag="VF517_04230"
     CDS             1386..1985
                     /locus_tag="VF517_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX8052177.1"
                     /translation="MRGPDPVDYAWWLSSRAAGVVAFALIARSVLLGLAMANRLVRGK
                     SIVKLHEHLALAGLVAIAVHGITLLGDSWLNPGFKGLLVPFTMEYKPFFTGLGILSGY
                     LAAVLGLSFYARRRIGAKRWRKLHRLTVLTYLLGVVHTLGAGTDASAPWMRAVMLATG
                     APIFFLFLLRVLPADPEPKPAPRAKPQPQRRPTPQPEAT"
     gene            1982..3163
                     /locus_tag="VF517_04235"
     CDS             1982..3163
                     /locus_tag="VF517_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8052178.1"
                     /translation="MTPPRHIVIAGAGLAAQRATQALRRRGHDGPIAIVGDEATVPYD
                     RPPLSKEYLSGAMEASALELRPSSWYADHGVELILDDPATSLDLAERRVNLASGARLG
                     YDRLLVATGARPRELPGTATFANVHVLRSAADAQRLRAVLRPGTRLAIAGAGFIGQEA
                     AATARSLGAEVTMIEAAPTPLFGQLGPRMGAWFAELHREEGARVLLGAQIASLRGDGD
                     ELTACVLADGTVVETDVLLVGIGVRAAGDWLAGSGLPTDGIPVDAAGRSGAPGVFAAG
                     DVSRPIDPATGEPGRSDHWEAAVAQAAAAAHGMLGLDPPRGPRPSFWSDQYGTRIQFA
                     GDARGADGVEIDGDPAARDFSALFHRGGVVRAGLLVGRPRALPKLREQLGQPIPDRRT
                     A"
     gene            3160..3372
                     /locus_tag="VF517_04240"
     CDS             3160..3372
                     /locus_tag="VF517_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX8052179.1"
                     /translation="MNAVARVDESACAAHGDCLVEAPQAFALNGDDIAEVIGSAPLDD
                     LTRAAESCPSVAISIFDQDTGEQLYP"
BASE COUNT          408 a         1400 c         1224 g          413 t
ORIGIN      
        1 gctgcgcggc ccgccaagtc cgccgccggc tcgcgcgccg cggccatcgc ctggagcggg
       61 tcggtcgcga cgtttgcggc cgccctcggg ttcttcgggt ggcaggtcgc cgccgggcgc
      121 gatcccgcca tcggcgcggg cccgcccgat tcgcagaagc cgaagaaggt gatcgtgcgc
      181 cggatcgtca agcgcacggt gatcatcacg acgcccgccc cctcgggtgg cggcggcggt
      241 ggcggcggca tgcccgccgg cggcgcggcc tccggcggcg gcgcggcgcc ctccggcggc
      301 ggagccccct ccggaggcgg tggcggctcg gcacccgcac ccgctcctgc cgccgccccg
      361 gcacccgcgc cccccgcgac cagcgcctcc tgaccccccg caccgacgcc gatcgaaccc
      421 atggccgaac acgccgacat cacattcacc tgcatgggct gcgacatgcg cgtgctcgtc
      481 gaggacgggc cgcgctcgca cgcggcggcg cgcggcgccc gccgccagct cgaagccatc
      541 gacgcgcagc tctcccgctt ccgcgaggac tccgagctcg cgcgcctgaa cgccgacccg
      601 cggttggtcg tccccgcctc gccgctgctg cgcgccgccg tgcgcgcggc gatctgggcc
      661 gccgaggcct ccggcggcct ggtcgacccg acgctgctcg gcgacctcga gcacgcgggc
      721 taccgccact cgctcaccgg catcgcccgc gtccccctgg ccgaggccct aaaggaggcg
      781 cccccgcgcg cggccgcccg cccgcgcggc gaccgccgct ggcgcgcgat ccgcgtggac
      841 gacgcgcgcg gcaccatcgc ccgcccaccc ggcctgcgca tcgacaccgg cggcaccacc
      901 aagggcctgg cggccgacgc cgtcgcgcac gtcctcggcg gcacgcgcca catcgtggac
      961 tgcgccggcg acctgcgtct cgacgccggc gcgggggatg ccttcggcgt gatcgtcgag
     1021 cacccgctca cgcgcgcgac ggccgacacg gtggacgtcc gcggcggagc catcgccacc
     1081 tccggacccg gccgccgcct ctggcgcgac gccgacggcg cgatcggcca ccacctgctc
     1141 gacgcggcca ccggcgcccc cgcctggacc ggtctcgttt ccgccacggc gctcgccccg
     1201 accgccctcg aggcggagac gctcgcgaag gccgcgctgc tcggcgggcc gaccgccgcg
     1261 cggcgcgtgc tcgcccgccg cggcggtgtc ctgatccacg acgacggcgc cacggagcgc
     1321 gtcggcccga tcgcgcgacc gcgactgcgg ctgcgcacca ccgaaagagg actggcggtg
     1381 gttctatgag gggcccggac ccggtcgact acgcctggtg gctgagcagc cgcgccgcag
     1441 gggtcgtcgc cttcgcgctg atcgcccggt ccgttctcct cggcctcgcg atggcgaacc
     1501 gcctggtgcg cggcaagtcg atcgtgaagc tccacgagca cctcgcgctc gcgggacttg
     1561 tcgcgatcgc cgtgcacggc atcacgctcc tgggcgacag ctggctgaac cccggcttca
     1621 agggcctgct cgtcccgttc accatggagt acaagccgtt cttcacgggg ctcgggatcc
     1681 tcagcggcta cctggcggcg gtgctcggcc tgtcgttcta cgcgcgccgc cggatcggcg
     1741 ccaagcgctg gcgcaagctg caccggctga cggtgctcac ctacctgctc ggcgtggtgc
     1801 acacgctcgg cgcgggcacc gacgcatcgg ccccctggat gcgcgccgtg atgcttgcca
     1861 ccggcgcccc gatcttcttc ctcttcctgc tgcgggtcct gcccgcggac cccgagccga
     1921 agccggcccc gcgcgcgaag ccgcagccgc agcggaggcc gaccccacaa ccggaggcga
     1981 catgacccca ccccgccaca tcgtcatcgc gggcgccggc ctcgcagcgc agcgcgccac
     2041 ccaggccctg cgccgccgcg gacacgacgg cccgatcgcc atcgtcggcg acgaggcgac
     2101 cgtcccctac gatcgcccgc cgctctcgaa ggagtacctg agcggagcga tggaggcgag
     2161 cgctctcgag ctccgcccgt cgagctggta cgcggaccac ggcgtcgagc tgatcctcga
     2221 cgaccccgcg acgagcctcg atctcgccga gcgccgcgtg aacctcgcga gcggagcccg
     2281 gctcggctac gaccggctgc tcgtcgcgac cggcgcccgc ccgcgcgagt tgcctggcac
     2341 cgcgacgttc gcgaacgtgc acgtgctgcg ctcggcggcg gacgcccagc gcctcagggc
     2401 ggtcctccgc cccgggacgc gcctcgcgat cgccggcgcc ggcttcatcg gccaggaggc
     2461 ggctgccacg gcgcgctcgc tcggcgccga ggtgacgatg atcgaggccg ctcccacccc
     2521 gctgttcggc cagctcggcc cgcgcatggg cgcgtggttc gcggagctgc accgcgagga
     2581 gggcgcgcgc gtgctgctcg gcgcgcagat cgcgagcctg cgcggcgacg gcgacgagct
     2641 gaccgcctgc gtgctcgcgg acggcaccgt ggtcgagacc gatgtcctgc tcgtcgggat
     2701 cggcgtgcgc gccgccggcg actggctcgc cggcagcggt ctgcccaccg acgggatccc
     2761 ggtcgacgcg gcgggacgca gcggcgcgcc cggcgtgttc gccgcaggcg acgtctcgcg
     2821 tccgatcgac ccggccaccg gtgagcccgg ccgcagcgat cactgggagg ccgccgtcgc
     2881 gcaggccgcg gcggccgcgc acgggatgct cgggctcgac cccccgcgcg gtccgcgccc
     2941 gagcttctgg agcgaccagt acggaacccg gatccagttc gccggggatg cccgcggggc
     3001 cgacggcgtc gagatcgacg gtgacccggc cgcgcgcgac ttcagcgcgc tcttccaccg
     3061 cggcggcgtg gtccgcgccg gcctgctggt ggggcgcccg cgagccctcc cgaagctgcg
     3121 cgagcagctc ggtcaaccca tccccgacag gaggacggca tgaacgcagt ggcacgagtt
     3181 gacgagagcg cctgcgcggc gcacggcgac tgcctggtgg aggcgccgca ggccttcgcc
     3241 ctgaacggcg acgacatcgc cgaggtcatc ggcagcgcgc ccctcgacga cctcacgcgc
     3301 gcggccgagt cgtgcccgtc ggtcgcgatc tcgatcttcg accaggacac gggtgagcag
     3361 ctctacccct gaccgatcgt cccctccagc cagtccacgg ccgcaggcac gtgcgccggg
     3421 tcgatgtggt gcgcggcgtc ggact
//
LOCUS       DASVPL010000091         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_364844, whole genome shotgun sequence.
ACCESSION   DASVPL010000091 DASVPL010000000
VERSION     DASVPL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364844"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..276
                     /locus_tag="VF517_04245"
     tRNA            204..276
                     /locus_tag="VF517_04245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Glu,seq:ttc)
     gene            315..791
                     /locus_tag="VF517_04250"
     CDS             315..791
                     /locus_tag="VF517_04250"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX8052180.1"
                     /translation="MRTWTEYESPIGRLTLRGDGGVLTAIHFPGRIGRLGDATRDPGA
                     FAEPVRQLDEYFAGERSEFRLDVVLDGTDFERAVWRELLKIPYGETRSYGALARAVGQ
                     PDEARAVGTAVGRTPVPIVVPCHRVVGSDGSLTGYGGGLDRKRSLLDLEAGQLALL"
     gene            complement(816..>1520)
                     /locus_tag="VF517_04255"
     CDS             complement(816..>1520)
                     /locus_tag="VF517_04255"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052181.1"
                     /translation="ASAPDQRTAMPLARHLSLAFPDDPTAAGRQRELMFGDWLLAAPV
                     LEPDARTRRLYLPPGRWIDLWRSVRYVERDGQLLMGRARVLSGGREETLPAPLDELPL
                     LARAGALIALAPRDVDTLADDLGAPGVVDLADRGRSRELLAFPRGSSEAWFDGDERLV
                     SREGRGRWTLQIRGKRKRTWGIAASLATLERPFRPCSVRAGGRPVRKWRWLAKERVLR
                     AMVKGKALTLDVRACR"
BASE COUNT          203 a          577 c          554 g          186 t
ORIGIN      
        1 cgccgacgag gccgcacgcg gaacgaatcc ggtcgatcgc gactcggacg acgacgggct
       61 gaccgacggc gacgaggtcg ctcgctcgac caacccgacg aaccccgaca ccgacggcga
      121 cgggatcccc gacggggtcg atcccaaccc gcgcagcccg ttcctcccct aacgccccgc
      181 acctagactc tgcctcccgc catgggggcg tggtctagtg gttaggacgc cggcctttca
      241 agccggaggt cacgggttcg atccccgtcg cccctacctc acataacgtc gtgcccgttc
      301 gttctaatgg gtgaatgcga acctggaccg agtacgagag cccgatcggg cggctgacgc
      361 tccgcggcga cggtggtgtt ctcacggcga tccacttccc gggccggatc ggacggctcg
      421 gcgacgccac gcgcgacccg ggcgccttcg ccgagccggt gcgccagctc gacgagtact
      481 tcgccggcga gcggagtgag ttcaggctcg acgtggtcct cgacgggacg gacttcgagc
      541 gggcggtctg gcgggagctg ttgaagatcc cgtacggcga gacgcgcagc tacggggcgc
      601 tcgcccgcgc ggtcggccag ccggacgagg cgcgcgccgt gggaacggcc gtcgggcgga
      661 cgccggtgcc gatcgtggtc ccgtgccacc gcgtggtcgg ctccgacggc tcgctcaccg
      721 gctacggcgg cgggctcgac cgcaagcgct cgctgctcga cctcgaggcc ggccagctcg
      781 cgctgctgta gtcgcgcggg ggcgcagccg cgcccctagc ggcacgcgcg cacgtcgagc
      841 gtcagcgcct tccccttcac catcgccctc agcacacgct ccttcgcgag ccagcgccac
      901 ttgcgcacgg gcctgccccc ggcgcgcacc gagcacggcc ggaacgggcg ctcgagcgtg
      961 gcgaggctcg ccgcgatgcc ccaggtgcgc ttgcgcttgc ccctgatctg gagcgtccag
     1021 cggccgcggc cctcgcgcga gacgagccgc tcgtcgccgt cgaaccacgc ctccgacgac
     1081 ccgcgcggga aggccaggag ctcgcggctg cgcccgcggt cggccaggtc cacgaccccg
     1141 ggcgccccga ggtcgtcggc gagcgtgtcg acgtcgcggg gcgcaagggc aatgagcgcg
     1201 cccgcgcggg cgagcagtgg cagctcgtcg agcggggccg gcagggtctc ctcgcgcccg
     1261 ccggacagca cccgcgctcg gcccatgagg agctggccgt cgcgctccac gtagcgcacg
     1321 ctgcgccaca ggtcgatcca gcggcccggc ggtagataca gccgccgcgt gcgcgcgtcg
     1381 ggctcgagca cgggcgccgc cagcagccag tcgccgaaca tcagctcgcg ctggcggccg
     1441 gccgccgtcg ggtcgtcggg gaacgcgagc gacaggtgcc gcgcgagcgg catcgcggtg
     1501 cgctggtccg gcgccgaggc
//
LOCUS       DASVPL010000092        13058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_246990, whole genome shotgun sequence.
ACCESSION   DASVPL010000092 DASVPL010000000
VERSION     DASVPL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13058
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246990"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VF517_04260"
     CDS             complement(<1..635)
                     /locus_tag="VF517_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8052182.1"
                     /translation="MEPVVAVHGGAGSGLRGGEGTLHREALAAAVEIARRALAEGAGA
                     LDAAVAAVVFLEDCPLFNAGRGAVPTEAGGYELDAALMDGADRRFGAVAAVTTVRNPV
                     LLARAVMERTPHVMLAGPGADAFAERAGLERAGPDWFEQRSEAHEPGDAGGVSSEPGD
                     AGGGSSEPGDAGDASSAGTVGAVVRDRDGRLAAATSTGGMRGQLPGRVGDTP"
     gene            complement(640..1464)
                     /locus_tag="VF517_04265"
     CDS             complement(640..1464)
                     /locus_tag="VF517_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX8052183.1"
                     /translation="MSRARRDVAIFLPAIAAVLVLYDKRGDLFPGLNREVRFATVVVL
                     VILGWAFARAIGRSLAPALFKRVDPATAGTIGFLIRLATITVALLVALRIAGLDARTL
                     AIGGAVTAVVLGLAAQQTVGNLFAGTVLLSARPFRVGERIRLQGGAIAGQIEGIVSSL
                     GLLYTTLAAGDDRIMIPNSVVLNMAITPMREPDAVELKARLPADVRPTAVQEMLDDTV
                     KVLTRSRPDIELQELDGDEVVVRITAAPERPADGAQLADEILSAISRFTRNGRPGG"
     gene            1644..2156
                     /locus_tag="VF517_04270"
     CDS             1644..2156
                     /locus_tag="VF517_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8052184.1"
                     /translation="MADEERNEQNEERDEEEASSSSDDEGSSGGGGGGTTVGDVMTKD
                     PQSVQPGDSIEDAAKKMKEADTGALLVVDGDELKGIVTDRDIVVNAVAAGNSDATVED
                     VASTDTTTTDPDSSLEDAIKLMRDNKVRRLPVVGDGGKPVGIVSLGDLAIEADDDSAL
                     KDISAAEPNA"
     gene            2166..2771
                     /locus_tag="VF517_04275"
     CDS             2166..2771
                     /locus_tag="VF517_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052185.1"
                     /translation="MRELAPGVFHWTALHPNLGIEVSSYFVAASGTLLNPLMPAEGIE
                     WFDGDRRPQRVLLTNRHHLRDSERFAEAFAIPIACHEAGLHEFSGGPAVKGFAWDEEV
                     APGITALEVGAICPEETALRIDTGDGFLAFADAVVNYGELGFAPDQYIGDDPPAIKRG
                     IRESVGRLLDATFDSLLPAHGDPVVGGGKEALRRFVEGGPE"
     gene            2857..3246
                     /locus_tag="VF517_04280"
     CDS             2857..3246
                     /locus_tag="VF517_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052186.1"
                     /translation="MYETLLFLHVLAAFALMAGIVMFTGTAFGVELGPRQLFVANRLT
                     DVGATVVLVFGVWIVLREDVYDITDGWILGAIVLWVIAVGLGAMTGRAMAESHGSRPE
                     GRAAVLHWATTAVTVAILVLMIWKPGV"
     gene            3243..3707
                     /locus_tag="VF517_04285"
     CDS             3243..3707
                     /locus_tag="VF517_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052187.1"
                     /translation="MSVIAALRPDDVDFPLFIHVLGAMVLVGSLLLAGVVLAGAWREG
                     AAAPTRTAYRVLLIGTLPAWLVTRVFAQILLDKPIYEPSEESTWVQIGFISTEPMLLV
                     LIGAIVAAGVGSRKALRENVAVGTSGRVAAGLTWLLLAVYVVTIWAMTTKPD"
     gene            3710..4189
                     /locus_tag="VF517_04290"
     CDS             3710..4189
                     /locus_tag="VF517_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8052188.1"
                     /translation="MPNLKRLEFDDVREGDGFEARRARLGLQTGVERVGASYWEVPPG
                     QAPYPYHFHLTEDEIVIVLEGKLSLRTPDGWRDLEEGEVLGFPVGEAGAHQLVNRGET
                     TARFLAVSNSGAPEAVVQPDANKIGVFERAPGGGGIREWYLRGDAVSYLEGVPPPES"
     gene            complement(4156..4848)
                     /locus_tag="VF517_04295"
     CDS             complement(4156..4848)
                     /locus_tag="VF517_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX8052189.1"
                     /translation="MPDLHVLRVFCAPDGSGGNPLGVFLDGSAIAPGRRLEVAAELGF
                     SETVFVDDPASGAIQIFTPGTELPFAGHPTVGTAWLLRHAGTPVQKLRPPAGEVHVRH
                     DGELTAVTARSEWAPPFNHHQLGSPAEVDALDGPPGGDELGAAWAWEDEAAGRIRSRV
                     FPVALGIDEDEATGAAAVRLCAELGRPIEIRQGRGSIIVARPVGDRIEIGGRVELDAV
                     RDYDSGGGTPSR"
     gene            4872..5627
                     /locus_tag="VF517_04300"
     CDS             4872..5627
                     /locus_tag="VF517_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8052190.1"
                     /translation="MPGTVTIERRGEVALVRIDRPPANAFDPGLLADGRAVLEELRAD
                     EPGAVVLTGRPGFFSAGVDLKLAPTLDPDGQREMVDGINRTFLGWYEFPRPVVAAVNG
                     HAIAGGLILALAADRRVGNGEGKLGLTELRAGIPYPAAAIAIVKAELTPPVARLLALG
                     AELVDQARGLDLGLLDEVVEPDSLLDRAVAVAEELAALPRSAYARVKEQLRGETAAAL
                     RAQLDRGEDPMLAGWLGDETAAASAAILERDSG"
     gene            5628..5804
                     /locus_tag="VF517_04305"
     CDS             5628..5804
                     /locus_tag="VF517_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052191.1"
                     /translation="MTGMTHENMKPGDEAPPEEPSAAKNTCEHCGGTGQHEGGETCPV
                     CQGTGEVQEAVGGG"
     gene            complement(5801..6565)
                     /locus_tag="VF517_04310"
     CDS             complement(5801..6565)
                     /locus_tag="VF517_04310"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX8052192.1"
                     /translation="MLKRMREQAATLRVTDHRPWPVPEGRPWVMAQSWHDLLFAHWRV
                     PAAALRPVIHPEIPIDEFDGSAWIGITPFRVEGSRPRLGPPPPLVSSFPECNVRTYAT
                     IDGKPGIWFFSLDAASRLAVHGARRAWRLPYFHADMAASTRGDAVAYRTDRVSPDGPQ
                     AAFEGTYRPSGPVRHALPGSLEHFLTERYCLYTLDGRGRVHRGDIHHPPWPLQPAEAE
                     LERNTMTAQVGLDLAGEPLLHFARRQDVLMWRIRPI"
     gene            6635..7378
                     /locus_tag="VF517_04315"
     CDS             6635..7378
                     /locus_tag="VF517_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8052193.1"
                     /translation="MKVRFYSIPGSHPSMAARLMLEAKGVEYKRTDMLSPFHRPVMRL
                     AGFPGNTVPAVNADGRRVQGTRAIAEWLDELRPDPPLFPADPERRRAVEEAERWGDQD
                     LQARTRRLSWWAMERDPSGVESFLQGARLGLPVPLLAKTARPAIWSAARANHVTEEAA
                     QADLAALPAAFDRVDGFIADGTIGGEQLNVADFQIATSIRLLMAFDDLRPSLEGRPVG
                     RHALRVVPDFPGRIPPLFDPAERAAALGA"
     gene            7440..10988
                     /locus_tag="VF517_04320"
     CDS             7440..10988
                     /locus_tag="VF517_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052194.1"
                     /translation="MPLFSRSPNDGPRRRRDDRADDAAWLSFDVDSFEFVPAGGGIGL
                     LRLSGRWSADPEQMVALPIEVEIERDGEAVRLEVMPDPSAPFAVGAPGGEEWRGAFMA
                     DVELAEDRRSDFALVAGGEVVAALPRPGDWEVVDEAIEAVAADEVDEADEAAASPARS
                     AADVLPELLAALDEVAALEEAAEDPPSLDPGAEELTARHLAAQADAERRADAAAAELA
                     EVQAELATLHDALHAAAAEAEREFATRAEHDATLAALRAELENERATNTQIQVALTDA
                     RAAAEREQATHEEIETALAEAHASAEAERTAREQADAALAEAHASAEAERTAREEADA
                     ALAEARASAEREQATREEIETALAELRTEIERERAAGAGSQAALAALHAELESLRHAE
                     EEASATLTDLQAEIDRERTARAEAEESVAAADAHAAREREARAEAEAAAAALRGELDA
                     ALSAATESDAARVEAETAAAALRDERETALAAATESAAALAEADAALTDARVEADRER
                     AARAEADASLSDARAETARERTLREEAEATLAESGTNLDDERAAREQAEAETTAAREE
                     IAAERTAREQAEAAAAAAAREEIAAERTAREEAEAGAAAMREEVAAERTAREQAERAV
                     AAARQEAEIASAELRADAENQRQAATAAVGALDALRAEFATERTAREEAEAALTELRS
                     EVGRHRAATENGESARIKLLAAADQERDRHQAAESELRGELEEARRELEATAAEAARS
                     RAALEGSQADRDAALHDVQAELDRVRADGERRDAEHEAALEELRTRLEDERAEHVTAV
                     ETMRAELDQARSDDRQVAEAAAAAEALRAELAAARAEHETTVEALRAELAEAGSEEAE
                     AADELRAELDRARAEHEAAADELRAELAEHATTVEALRAELAEAGSEEAAAADELRAE
                     LAAARAEHENTVEALRAELAEAVPAEDPAAADELRAELDRARAELAAASAEHADALEE
                     LRAALADADAETERRVAGEAATVEALRAELDAVGDERRAGEDTTIAALREEHEAELAA
                     LRDELERERADARRQVDDQGVEIEELRTRIEEERAVTAEAAESATGEQSDHDLPALRN
                     ELTQVRRDLEIERRRRVISDEELRVHLSMERQLRATIAGPDADFAGTDANLIRRVRAA
                     EQRRDARDSADEAPVLDTEDFFARLEQAKRLSENPV"
     gene            complement(10985..>13058)
                     /locus_tag="VF517_04325"
     CDS             complement(10985..>13058)
                     /locus_tag="VF517_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX8052195.1"
                     /translation="THGPTTDWGGGFRMTWDGTSATTPHVAGVAALVLARGRAQGLDL
                     SAAEVMQVVRMSADDLADPAKGYAPGWDLLSGWGRVNAFAAVERAAPGRVPPVADIQA
                     PEWYEPVRGDVVVTGIARGRSAVSWQLEIGDGQQPSEWRAIGSGTSTAAGGARLGRVA
                     AGALGAGPHTLRLRVTDADGNVGEDRALFHALRDPALRRGFPRRLGTSGEASPVLADL
                     DRDGAQDIVLATSDGEVRVLSGRTGRPLRGWPRRMKATPGSGAAGRRIGTLRPGILAT
                     PAIGDVTGGREPEMLAAGLDGRLYGWTARGRPLRGFPYRIRLRRPAENGRLDAAIYAS
                     PALADLDGDRKLDAIFGAADQRVYAVRGNGRSVRGWPVLARDTAAGGGDGAAAKILSS
                     PAVGDLDGDGRPDVVEGTGEAYGSTPSMSGRVHAWNAHGEVLAGWPVAPSGLAVNSIP
                     LVGLGVPMSPVLADVDGDGRDEVAAAAFTGEPELYRGDGSRMSGAGGDGHFAFAGRGP
                     SSPSGSPGAIALGANALFGPARPGGPLRLFGGMVDSRLAAAQSSPATRVEFEHLVGGW
                     DAASGQWLDAWPRPIEGWTIVAGPALADVDGDGAAELIAGSSGNVLHAFREDGSEPSG
                     WPKDTAGWLLAAPAVGDVDGDKRLEVVAVTRDGWLYVWDTPARATAAGAPWPSFRHDV
                     RNTGRYGG"
BASE COUNT         1767 a         4970 c         4795 g         1526 t
ORIGIN      
        1 ggcgtgtcgc cgacccggcc ggggagctgg ccgcgcatcc cgcccgtaga ggtcgcggcg
       61 gcaagccggc cgtcgcggtc gcgcaccacg gcgcccacgg tgcccgcgga ggaggcgtcc
      121 ccggcatcgc ccggctcgga cgagccgccg cccgcgtcgc ccggctcgga ggagacgccg
      181 cccgcatcgc ccggctcgtg cgcctccgag cgctgctcga accagtccgg cccggcccgc
      241 tcgagcccgg cgcgctcggc gaaggcgtcg gcgccgggtc ccgcgagcat cacgtgcggc
      301 gtcctctcca tcaccgcgcg agcgagcagc accgggttgc gcaccgtcgt gaccgcggcc
      361 acggcgccga agcgccgatc ggccccgtcc atcagggccg cgtcgagctc gtaaccgccg
      421 gcctcggtgg gcaccgcccc gcgcccggcg ttgaacagcg ggcagtcctc gaggaacacg
      481 acggcggcca ccgccgcgtc gagcgccccc gcaccctccg ccagcgctcg gcgggcgatc
      541 tccaccgcgg cggcgagtgc ctcccggtgc agcgtgccct cgccgccgcg cagaccgctg
      601 ccggcgccgc cgtgcaccgc cacgacgggc tccaagccct cagccgcccg gccggccgtt
      661 gcgcgtgaag cgcgagatcg cgctcaggat ctcgtcggcg agctgtgcgc cgtcggccgg
      721 ccgctccggc gcagccgtga ttcgcaccac cacctcgtcc ccgtcgagct cctggagctc
      781 gatgtcgggc cgcgaccgcg tgagcacctt cacggtgtcg tcgagcatct cctgcaccgc
      841 cgtcggccgc acgtcggccg gcagccgcgc cttgagctcc accgcgtcgg gctcgcgcat
      901 cggcgtgatc gccatgttca gcacgacgct gttggggatc atgatccggt cgtcgcccgc
      961 ggcgagcgtc gtgtagagca ggccgagcga gctgacgatc ccctcgatct ggccggcgat
     1021 cgcgccgccc tggagcctga tgcgctcgcc cacccggaac ggtcgcgcgc tcagcagcac
     1081 ggtgccggcg aacaggttgc ccacggtctg ctgcgccgcg agccccagca ccacggccgt
     1141 cacggcgccg ccgatcgcga gcgtgcgcgc gtcgagcccc gcgatacgca gcgccacgag
     1201 cagcgcgacc gtgatcgtcg cgaggcggat gaggaagccg atcgtgcccg ccgtggccgg
     1261 gtcgacgcgc ttgaacagcg cgggcgcgag gctgcgcccg atcgcccgcg cgaatgccca
     1321 gccgaggatc acgagcacca cgacggtggc gaagcgcacc tcgcggttga gtcccgggaa
     1381 caggtcgccg cgcttgtcgt agagcacgag cacagccgcg atcgccggca ggaagatcgc
     1441 cacgtcgcgg cgtgcgcggc tcacccgttg cgggctcaac tcccgctcca ggccgacctc
     1501 gcgccacgca tgggtgcgcg tctcgaacat gtcgcgcatg gggatttccg ggcgttttct
     1561 cggcaccgcc cctagggata gcaacccagc ccatcgggta cgggtgacgg gaccgcacga
     1621 ccccgacatg ggaggcacac gacatggccg acgaggaacg caacgagcag aacgaggagc
     1681 gcgacgagga ggaggcctcg tcgtcgagcg acgacgaggg ctcgtcgggc ggaggcggcg
     1741 gcgggaccac cgtcggcgac gtgatgacca aggacccgca gtcggtccag cccggcgact
     1801 cgatcgagga cgccgccaag aagatgaagg aggccgacac cggcgcgctg ctcgtcgtcg
     1861 acggcgacga gctcaagggc atcgtgaccg accgcgacat cgtggtgaac gcggtggccg
     1921 ccggcaactc cgacgcgacc gtcgaggacg tggcgtccac cgacacgacc acgaccgacc
     1981 ccgactcctc gctcgaggac gcgatcaagc tgatgcgcga caacaaggtc cgccggctgc
     2041 ccgtcgtggg tgacggcggc aagcccgtcg gcatcgtctc gctcggcgac ctcgccatcg
     2101 aggccgacga cgactcggcg ctcaaggaca tcagcgcggc ggagccgaac gcgtaggcga
     2161 tggcgatgag ggagctggcc ccgggcgtct tccattggac cgcgctacat ccgaacctcg
     2221 ggatcgaggt cagctcctac ttcgttgcgg cctccggcac gctgctgaac ccgctcatgc
     2281 ccgccgaggg catcgagtgg ttcgacggcg accggaggcc gcagcgcgtc cttctcacca
     2341 accgccacca cctgcgcgac agcgagcgct tcgcggaggc gttcgcgata ccgatcgcct
     2401 gccacgaggc cggcctgcac gagttcagcg gtggcccggc cgtcaagggg ttcgcgtggg
     2461 acgaggaggt ggcgcctggg atcacggccc tcgaggtcgg cgcgatctgc cccgaggaga
     2521 ccgccctgcg gatcgacacc ggcgacggct tcctcgcgtt cgccgacgcc gtcgtcaact
     2581 acggcgagct cggcttcgcg ccggaccagt acatcgggga cgacccgccg gccatcaagc
     2641 gcgggatccg cgagtcggtg ggccggctcc tcgacgcaac gttcgactcg ctgctccccg
     2701 cccacggtga ccccgtggtg ggagggggca aggaggcgct gcgccgtttc gtggagggcg
     2761 ggccggagta gcggcgcgcg acgtgatcga tcgcgtcgca cttgaggtct tcgagctcct
     2821 cggtgccgac cggcccgccg cttagtctcc gcgcgcgtgt acgagacgct cctctttctg
     2881 cacgtcctcg ccgccttcgc gctgatggcg gggatcgtga tgttcaccgg gacggcgttc
     2941 ggcgtcgagc tgggcccacg gcagctcttc gtcgcgaacc ggctcaccga cgtgggggcc
     3001 accgtcgtgc tcgtgttcgg cgtgtggatc gtgctccggg aggacgtcta cgacatcacc
     3061 gacggctgga tcctgggcgc gattgtgctg tgggtgatcg cggtcggcct gggggccatg
     3121 accgggcgcg ccatggccga gtcccacggc tcgcgtcctg aggggagggc ggccgtcctc
     3181 cactgggcga ccaccgccgt caccgtcgcg atcctcgtgt tgatgatctg gaagcccggc
     3241 gtatgagcgt gatcgcggcc ctccgcccgg acgacgtcga ctttccgctg ttcatacacg
     3301 tgctcggcgc gatggtgctg gtcggctcgc tgctgctggc gggcgtcgtg ctcgcgggcg
     3361 cctggcgtga gggagccgcg gcgcccacgc gcacggccta cagggtgctg ctgatcggga
     3421 cgctgccggc gtggctcgtc acgcgtgtgt tcgcgcaaat cctgctcgac aagccgatct
     3481 acgagccctc cgaggagtcc acctgggtcc agatcgggtt catctcgacc gagccgatgc
     3541 tcctggtgct gatcggcgcg atcgtcgcgg cgggcgtcgg gtcgcggaag gcgctgcgcg
     3601 agaacgtcgc cgtgggcacg tcgggccgcg tggccgccgg gctgacctgg ctgcttctcg
     3661 ccgtctatgt cgtcaccatc tgggcgatga ccaccaagcc ggactagcga tgccgaacct
     3721 gaagcgactc gaattcgacg acgtccgtga gggggacggc ttcgaggccc ggcgggcgcg
     3781 gctcggcctt cagaccggcg tcgagcgcgt cggggcgagc tactgggagg tgccgcccgg
     3841 ccaggctccc tacccgtacc acttccacct caccgaggac gagatcgtga tcgtgctgga
     3901 gggcaagctg agcctgcgca cgccggacgg ctggcgcgat ctcgaggagg gcgaggtcct
     3961 cggcttcccg gtgggggagg ccggggcgca ccagctggtc aaccgcggcg agacgaccgc
     4021 gcgcttcctc gcggtgagca actccggcgc gcccgaggcg gtggtgcagc ccgacgcgaa
     4081 caagatcggg gtgttcgagc gcgcgcccgg tggcggcggg atccgcgagt ggtacctccg
     4141 cggcgacgcc gtgtcctacc tggagggcgt gccgccgccc gagtcgtagt cgcggaccgc
     4201 gtcgagctcg acgcggccgc cgatctcgat gcggtccccg accgggcgcg cgacgatgat
     4261 cgagccgcgg ccctggcgga tctcgatcgg gcggcccagc tccgcgcaca gccgcaccgc
     4321 cgccgcgccg gtggcctcgt cctcgtcgat gcccagggcg acggggaaca cgcgcgagcg
     4381 aatgcggccg gcggcctcgt cctcccacgc ccacgcggcg cccagctcgt cgccgccggg
     4441 cggtccgtcg agcgcgtcca cctccgccgg ggagccgagc tggtggtggt tgaacggcgg
     4501 ggcccattcg ctgcgggcgg tcacggccgt cagctcgccg tcgtggcgca cgtgcacctc
     4561 gcccgcgggc gggcgcagct tctgcaccgg cgtgccggcg tgacgcagca gccaggcggt
     4621 gcccacggtc gggtggcccg cgaacggcag ttcggtcccg ggggtgaaga tctggatcgc
     4681 cccgctggcg gggtcgtcca cgaagacggt ctcgctgaag ccgagctcgg cggccacctc
     4741 caggcgccgg cccggcgcga tcgcggagcc gtcgaggaac acgccgagcg ggttgccgcc
     4801 gctgccgtcg ggggcgcaga acacgcgcag gacgtggagg tcgggcacgc cgcgagggta
     4861 ccctccggcc ggtgcccggc acggtgacca tcgagcggcg cggcgaggtc gccctggtgc
     4921 ggatcgaccg cccacccgcg aacgcgttcg acccggggct gctggccgac ggccgcgccg
     4981 tactcgagga gctgcgcgcc gacgagccgg gggccgtcgt gctgaccggg aggccggggt
     5041 tcttctcggc cggggtcgac ctgaagctcg cgccaacgct cgaccccgac ggccagcgtg
     5101 agatggtgga cggcatcaat cggaccttcc tcggctggta cgagttcccg cgaccggtgg
     5161 tggccgctgt gaacggccac gccatcgcgg gcggcctcat cctcgcgctg gcggccgatc
     5221 gccgggtggg caacggcgag ggcaagctcg gcctcaccga gctgcgcgcc ggcatcccgt
     5281 acccggccgc cgcgatcgcc atcgtgaagg ccgagctgac gccgccggtc gcgcggctgc
     5341 tggccctcgg ggcggagctg gtcgatcagg cgcgcggcct cgatctcggg ctgctcgacg
     5401 aggtggtcga gccggactcg ctcctcgatc gggcggtcgc ggtggcggag gagctcgccg
     5461 cgctgccccg ctcggcctac gcgcgcgtga aggagcagct ccgcggtgag accgcggcgg
     5521 ccctgcgcgc gcagctcgac cgcggcgagg acccgatgct cgccggctgg ctcggcgacg
     5581 agacggccgc cgccagcgcc gcgattcttg agcgcgactc cgggtaggtg accggcatga
     5641 cccacgagaa catgaagccg ggcgacgagg ctccccccga ggagccgtcg gccgcgaaga
     5701 acacctgcga gcactgcggc ggcaccgggc agcacgaggg cggcgagacc tgtcccgtgt
     5761 gtcagggcac gggcgaggtc caggaggccg tcggcggcgg ctagatcggc ctgatcctcc
     5821 acatcagcac gtcctggcgg cgcgcgaagt ggaggagcgg ctcgccggcg aggtcgagtc
     5881 cgacctgcgc cgtcatggtg ttgcgctcca gctcggcctc ggccggctgc agcggccacg
     5941 gcgggtgatg gatgtcgccg cggtgcacgc ggccgcggcc gtcgagcgtg tataggcagt
     6001 agcgctcggt gaggaagtgc tcgagcgagc cgggcagcgc atgcctgacc ggtccagacg
     6061 ggcggtaggt cccctcgaat gcggcctgcg ggccgtcggg cgagacgcgg tcggtgcggt
     6121 acgcgacggc gtcaccgcgc gtggaggcgg ccatgtcggc gtggaagtag ggcagccgcc
     6181 aggcgcggcg ggcaccgtgc accgcgaggc ggctggccgc atcgaggctg aagaaccaga
     6241 tgcccggctt gccgtcgatg gtcgcgtagg tgcgcacgtt gcactcgggg aagctcgaca
     6301 cgagcggcgg cggggggccg aggcgcgggc gcgagccctc cacccggaac ggcgtgatgc
     6361 cgatccacgc gctgccgtcg aactcgtcga tcgggatctc ggggtggatg accggacgca
     6421 gcgcggcggc gggcacgcgc cagtgggcga atagcaggtc gtgccagctc tgtgccatga
     6481 cccacgggcg tccctcgggc accggccacg gccggtggtc ggtcacccgc agcgtcgctg
     6541 cctgctctcg catccgcttc agcacggtgg cgcccgacgc tatccgttcg catgtcacgc
     6601 ccgctgcccc cgggcaggcc gtacgatccg ggtcgtgaag gtccgcttct actcgatccc
     6661 cgggtcgcac ccgtcgatgg cggcgcggct gatgctcgag gcgaagggcg tggagtacaa
     6721 gcgcaccgac atgctctcgc cgttccaccg ccctgtcatg cgcctcgcgg gattccccgg
     6781 gaacacggtg ccggccgtga acgcggacgg tcgcagggtc cagggcacgc gcgcgatcgc
     6841 cgagtggctc gacgagctgc gacccgaccc gccgctcttc cccgccgacc ccgagcgccg
     6901 acgtgcggtc gaggaggccg agcgctgggg cgaccaggac ctccaggcgc gaacgcggcg
     6961 cctgtcgtgg tgggcgatgg agagggatcc ctcgggcgtg gagagcttcc tccagggagc
     7021 gcggctgggg ctgccggtgc cgctgctggc caagacggcg cgacccgcga tctggtcggc
     7081 cgcgcgcgcc aaccacgtga ccgaggaggc agcgcaggct gacctcgccg cgctgccggc
     7141 ggcgttcgac cgcgtcgacg gtttcatcgc cgacggcacg atcggcggtg agcagctgaa
     7201 cgtggccgac ttccagatcg ccacctcgat ccgcctgctg atggcattcg acgacctgcg
     7261 cccgtcgctc gaagggcgtc ccgtggggcg gcacgcgctg cgcgtcgtgc cggatttccc
     7321 ggggcggatc ccgccgctgt tcgacccggc ggagcgagcg gcggcgctgg gcgcgtagac
     7381 ccggttccga cgggggttca agccaggggc ggttccgtgc cgatacaggc caggcccgga
     7441 tgcccctgtt ctcccgctca cccaacgacg gcccccgccg gcggcgcgac gaccgcgccg
     7501 acgatgccgc atggctgtcg ttcgacgtcg attccttcga gttcgtgcca gccgggggcg
     7561 gcatcgggct gctgcgactg agcggccgct ggtcggcgga tccggagcag atggtggcgc
     7621 tcccgatcga ggtcgagatc gagcgtgacg gcgaggccgt gcggctggag gtcatgccgg
     7681 acccgtccgc gcccttcgcc gtcggcgcgc ccgggggcga ggagtggcgc ggagccttca
     7741 tggcggacgt cgagctggcg gaggaccgcc gctccgattt cgcgctcgtg gccggtggcg
     7801 aggtcgtcgc cgcgctgccg cggccggggg actgggaggt tgtggacgag gccattgagg
     7861 ccgtcgcagc tgacgaagtc gacgaggccg acgaggccgc cgcgagcccg gcccgcagcg
     7921 ccgccgacgt gctgcccgag ctgcttgcgg ccctcgacga ggtggcggcg ctggaggagg
     7981 ccgccgagga tcccccctcg ctcgacccgg gcgctgagga gctgaccgca cggcacctgg
     8041 ccgcgcaggc ggacgcggag cgccgcgccg acgcggccgc cgccgagctc gccgaggtcc
     8101 aggccgagct ggcgaccctt cacgacgccc tgcacgccgc cgccgccgag gccgagcgcg
     8161 agttcgcgac ccgcgcggag cacgacgcga ccctggccgc cctccgggcg gagctcgaga
     8221 acgagcgcgc gaccaacacg cagatccaag tcgccctcac cgatgcccga gccgccgccg
     8281 agcgcgagca ggccacccac gaggagatcg agaccgccct cgccgaggcc cacgcttccg
     8341 ccgaagccga gcgcaccgcc cgcgagcagg ccgacgccgc cctcgccgag gcccacgctt
     8401 ccgccgaagc cgagcgcacc gcccgcgagg aggccgacgc cgccctcgcc gaggctcgcg
     8461 cctccgccga gcgcgagcag gccacccgcg aggagatcga gaccgccctc gccgagctgc
     8521 gcacggagat cgagcgcgag cgtgccgcgg gcgccggttc gcaggcggcc ctggctgcac
     8581 tgcatgccga gttggagagc ctgcgccacg ccgaggagga ggcgagcgcg acgctcacgg
     8641 acctccaggc cgagatcgac cgcgagcgca ccgcccgcgc cgaggccgag gagtcggtcg
     8701 cggccgccga cgcccatgcg gctcgcgagc gcgaggcccg cgccgaggcc gaggcagccg
     8761 ccgcggccct ccgcggcgag ctcgatgcag cactctccgc cgcgacggag tccgacgcgg
     8821 cccgcgtcga ggccgagacg gccgccgccg cccttcgcga cgaacgcgaa accgcacttg
     8881 ccgccgctac cgagtccgcc gcggccctag ccgaggccga cgccgcgctc accgacgccc
     8941 gcgtcgaggc cgaccgcgag cgtgccgcac gcgccgaggc cgacgcctcg ctgagcgacg
     9001 cccgcgccga gacggcccgc gagcggacgc tccgcgaaga ggccgaggca acgctggccg
     9061 agtccggcac gaacctcgat gacgagcgcg ccgcacgcga gcaggccgag gccgagacca
     9121 ccgccgcccg ggaggagatc gccgccgagc gcaccgcacg cgagcaggcc gaggccgccg
     9181 ccgccgccgc cgcccgcgag gaaatcgccg ccgagcgcac cgcacgcgaa gaggccgagg
     9241 ctggtgccgc agcgatgcgc gaggaggtcg ccgccgagcg caccgcacgc gagcaggccg
     9301 agcgtgccgt cgccgccgcc cgtcaggagg ccgaaattgc cagtgccgag ctgcgcgccg
     9361 acgccgagaa ccagcgtcag gccgccaccg cggcggtcgg tgccctcgac gccctgcgcg
     9421 ccgagttcgc caccgagcgt accgcacgcg aagaggccga ggccgctctc acggagctgc
     9481 gctccgaggt cggccgccac cgcgcggcca ccgagaacgg cgagtccgct cgcatcaagc
     9541 ttcttgccgc cgccgatcag gagcgcgatc gtcatcaggc cgccgagtcg gagctgcgcg
     9601 gcgagctcga ggaggcacgg cgcgagctcg aggcgacggc tgccgaggcg gcgcgctcgc
     9661 gcgcggcact ggagggctcg caggcagacc gcgacgccgc gctccacgat gtacaggcag
     9721 agctcgaccg cgtgcgcgcc gacggcgagc gacgcgatgc cgagcacgag gcggctctgg
     9781 aggaacttcg cacccgcctc gaagacgagc gcgccgagca cgtcacggcg gtcgagacga
     9841 tgcgtgccga gctcgatcag gcccgctcgg acgaccgcca ggtcgccgag gcggcggctg
     9901 ccgcagaggc cctgcgcgcg gagcttgccg ccgcccgcgc cgagcacgag accaccgtcg
     9961 aggccctgcg cgccgagctc gccgaggccg gctccgagga ggcggaagcg gccgacgaac
    10021 tgcgcgccga gctcgatcgg gctcgtgccg agcacgaggc tgccgcggac gagctgcgcg
    10081 ccgaactcgc cgagcacgcg accaccgtcg aggcgctgcg cgcggagctt gccgaggccg
    10141 gctccgagga agcggccgcc gccgacgagc tgcgcgcgga gcttgccgcc gcccgcgccg
    10201 agcacgagaa caccgtcgag gcgctgcgcg ccgagcttgc cgaggccgtc ccggcggagg
    10261 acccggcagc cgccgacgaa ctgcgcgcgg agctggatcg ggcccgcgcc gagcttgccg
    10321 ccgccagcgc tgagcatgcc gatgcgctcg aggagctgcg ggctgccctg gccgacgccg
    10381 acgctgagac cgagcgccgc gtggcagggg aggccgcgac cgtcgaggcc ctcagggccg
    10441 agctcgatgc cgtgggcgac gagcgccgcg ccggcgagga caccaccatc gccgccctgc
    10501 gcgaggagca cgaggccgag ctggccgccc tccgcgacga gctcgagcgc gagcgcgccg
    10561 acgcccggcg gcaggtcgac gatcagggtg tcgagatcga ggagcttcgg acccggatcg
    10621 aggaggagcg cgccgtcacc gccgaggccg ccgagagcgc cacgggggag cagtcggacc
    10681 acgatctccc cgccctgagg aacgagctga cgcaggtgcg ccgcgacctc gagatcgagc
    10741 gccgccgccg cgtgatcagc gacgaggagc tacgtgtcca cctctcgatg gagcgccagc
    10801 tccgcgccac catcgccggg ccggacgccg acttcgcggg caccgacgcc aacctcatcc
    10861 gccgcgtccg cgccgccgag cagcgccgcg acgcccggga ctccgccgac gaggcccccg
    10921 tgctcgacac cgaggacttc ttcgcgcgcc tcgagcaggc caagcggctg agcgagaacc
    10981 cggtctagcc gccgtaccgc ccagtgttcc gcacgtcgtg gcggaacgag ggccacggcg
    11041 ccccggcggc cgtcgcccgc gccggtgtgt cccagacgta gagccagccg tcccgcgtca
    11101 cggccaccac ctcgagccgc ttgtcgccgt ccacgtcccc gacggcgggc gccgccagca
    11161 gccacccggc cgtgtccttc ggccacccgg acggctccga cccgtcctcg cggaacgcgt
    11221 gcagcacgtt cccgctcgac ccggcgatca gctcggccgc tccgtcgccg tcgacgtcgg
    11281 ccagtgccgg ccccgccacg atcgtccagc cctcgatcgg ccgcggccag gcgtcgagcc
    11341 actgcccgct ggcggcatcc catccgccca ccaggtgctc gaactcgacc ctggtcgccg
    11401 gcgaggactg cgccgccgcc agtcgcgagt cgaccatccc gccgaacagc cgcagcggcc
    11461 caccgggccg tgccggcccg aacagcgcat tcgcaccgag cgcgatcgcg cccggcgagc
    11521 ccgagggcga gctcggccct cgcccggcga acgcgaagtg gccgtcgccc ccggccccgc
    11581 tcatccgcga gccgtcgccg cggtagagct cgggctcgcc tgtgaacgcc gccgccgcca
    11641 cctcgtcgcg tccgtcgcca tccacgtccg ccagcaccgg cgacatcggc actcccagcc
    11701 ccaccagcgg gatcgagttc accgcgagcc cgctcggcgc caccggccag cccgcgagaa
    11761 cctccccgtg cgcgttccag gcgtggaccc ggccgctcat cgacggcgtc gacccatacg
    11821 cctcgccggt gccctcgacc acgtcgggcc ggccatcccc gtcgaggtcg ccgacggcgg
    11881 gggaggagag gatcttcgcc gccgcgccgt cgccaccgcc ggccgctgta tcgcgtgcca
    11941 gcaccggcca gccgcgcacc gaccggccgt tgcccctcac ggcgtagacg cgctggtcgg
    12001 cggcgccgaa gatcgcgtcg agcttgcggt cgccgtcgag gtccgcgagc gccgggctcg
    12061 cgtagatcgc cgcgtcgagc cgaccgttct cggccggccg ccgcaaccgg atccggtacg
    12121 ggaacccgcg cagcggacgc ccgcgagcgg tccacccgta gagccgcccg tcgagccccg
    12181 ccgccagcat ctccggctcg cgcccgccgg tcacgtcgcc gatcgccggc gtggccagga
    12241 tcccgggccg gagcgtcccg atccgccgtc ccgcggcacc cgacccgggg gtcgccttca
    12301 tgcgtcgcgg ccacccgcgc agcggccgtc ccgtccgccc cgagagcacg cgcacctcgc
    12361 cgtcgctcgt ggcgagcacg atgtcctgcg caccgtcgcg gtcgaggtcc gccagcaccg
    12421 gtgacgcctc acccgacgtc cccagccgcc gcgggaagcc gcgccgcagc gccgggtccc
    12481 gcagcgcgtg gaagagcgcg cggtcctcgc ccacgttgcc gtcggcgtcc gtgacccgca
    12541 gccgcagcgt gtgcggcccg gccccgagcg caccggccgc cacgcgcccg agccgggcac
    12601 cgccggccgc cgtggacgtc ccggacccga ttgcccgcca ctcgctcggc tgctgcccgt
    12661 cgccgatctc gagctgccag ctcaccgccg agcgcccgcg cgcgatgccc gtcaccacca
    12721 cgtcaccgcg caccggctcg taccactcag gggcctggat gtcggccacc ggcggcaccc
    12781 gccccggcgc ggcgcgctcc accgccgcga acgcgttcac ccggccccag cccgagagca
    12841 ggtcccaccc gggcgcgtag cccttcgccg gatcggcgag gtcgtcggcg ctcatgcgca
    12901 cgacctgcat cacctccgcc gccgagaggt cgaggccctg cgcgcgcccg cgcgcgagaa
    12961 cgagcgccgc cacgcccgcc acgtggggcg tcgtcgccga ggtcccgtcc caggtcatcc
    13021 tgaagccgcc gccccagtcg gtcgtggggc cgtgggtc
//
LOCUS       DASVPL010000093        18193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_247474, whole genome shotgun sequence.
ACCESSION   DASVPL010000093 DASVPL010000000
VERSION     DASVPL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18193
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247474"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VF517_04330"
     CDS             <1..849
                     /locus_tag="VF517_04330"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX8052196.1"
                     /translation="DEFGERRVRDTPISENTIVGMGVGAAMAGLRPVVEIMTVNFALL
                     AMDQIVNCAASIRYMFGGQVTVPMVIRMPQGAGHQLGPTHSHSFEALYLHVPGLLVAV
                     PSTPEDARGLLKAAIRDDNPVIFIEHETLYGKKGEVPDEAEPMRFGEAAIRREGEDVT
                     IVGISRMAHTAADAAETLAEEHEIEAEVIDPRTLRPLDLDTILESVRKTNRAVIVEEG
                     WPHGGVGANLAALIQEQAFDHLDAPIERVTGADVPMPYSKPLEQAAFPHSEHVVQAAL
                     ATFRDV"
     gene            complement(1580..2293)
                     /locus_tag="VF517_04335"
     CDS             complement(1580..2293)
                     /locus_tag="VF517_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052197.1"
                     /translation="MARSWPAAAAAIATVLAAFAAPPSPAGAAPRDVSTFVPSYAEPY
                     ASESNSFYSWSGPVLAGGSAAWARSRPQGGWVVERAAPGQPVTRLATSERPTIGSRVI
                     HTVRPAGGSASRVAWTDTAFDVLDAHGMSYRSIRSRLYATGAGGASIVTGCGDGPSEC
                     RCPQFCSNFSYAADLDGDVLAYVEGNYPHRTIVVDDLASADAPVRIEVGGDAGDHIRI
                     AGDHVAYVGFSNLGSHTVV"
     gene            2356..3699
                     /locus_tag="VF517_04340"
     CDS             2356..3699
                     /locus_tag="VF517_04340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX8052198.1"
                     /translation="MATDVTMPRLSDSMEEGTILKWLVEQGGEVKKGEPLVEIETDKA
                     NMTYDADTSGVLIEVVAGEGDTLPIGEVIARIGEAGEKPAEDSGDSGADDQEEPEEAE
                     EPEEAEEPKEKPKREPQPQASGASNPVGGPGGGGGGNGGSADGERVKASPVARRVAKD
                     LGIELAQLEGTGPGGRIVKADVEAAAQGNGKPQDEAPPAEAEKEEAPAEAPAPKADAG
                     AKGEVETQELSRLQKTVSRRMAESKATAPDYTLHMQIDMTLCVELRKRLKEVEDPAPS
                     FNDMIVKACANALREHPRVNGAYRDGRFELYGNVNVGVAVAASDALVVPTIFEADKKS
                     LGEIARQARALIEKVRDGKITPPEVSGGTFTVSNLGMYGVDEFSAVINPPQAAILTVG
                     SLKKRPVVDGDGRVVARDTLTLSMVSDHRILYGADAAQFLARVRELLEQPLALAL"
     gene            3699..4073
                     /locus_tag="VF517_04345"
     CDS             3699..4073
                     /locus_tag="VF517_04345"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEX8052199.1"
                     /translation="MLRALVLVLSAAALLAGCDDEGDGVVFKDPKGEIAVEEGMEFTL
                     ELRVNASVGYDWVPVAPGRSGPVRLKATSVDYPNEESIGDSGFKRFTYEATRTGQGTI
                     ELRRLFRGDPQERRLLIVRVRG"
     gene            complement(4070..5122)
                     /locus_tag="VF517_04350"
     CDS             complement(4070..5122)
                     /locus_tag="VF517_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052200.1"
                     /translation="MPTFDRLADLPIQIDGYALEGLEADVSSSFTRLSTLVRMSGGGE
                     EGVGEDVVYDALDHIAFQATGPVHDLTGPRTFGELCELTGSLDLFEDSPPVRDVSRLY
                     RRWAFESAALDLALRQAGESLAARLGLEPRPVTFVVSLRLGDPPTLEPITSRLASYPT
                     LRFKLDPTSDWSEELIQELVATGAVDSVDFKGYYVGTPVDQAADPDLYRRVAEAFPDA
                     WIEDPALTDETDPVLEPYRDRITWDAPIHSIADIESLPFAPRMVNIKPSRVGGVADVC
                     ATYDYCAEHGIGAYGGGQFELGAGRGHIQYLASLFHPDTPNDVAPSGFNLPDPEPGLP
                     VSPLEPQIAPAGFRWG"
     gene            complement(5134..5838)
                     /locus_tag="VF517_04355"
     CDS             complement(5134..5838)
                     /locus_tag="VF517_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052201.1"
                     /translation="MDTTELTRVVIALPAAGVVPAMVPRLGRAGRALAAVPLAMWAAA
                     PTLDDWRGWALVALAVVAAQLARSIDGEVRESFARAVLCTVVLAGASALLLDGSAAAE
                     SIEDALLDDQVAIVAAGALLTVFVGGAAIAWLLTPFAALVHETAADAGVVTPTRAGLY
                     IGWLERTLIYGFVVAGAPGAVAVVVAVKSIARFPSFTEEKFAEYFLIGTLASVVVAVG
                     LGVAVRAILGLMPVLA"
     gene            complement(5820..6419)
                     /locus_tag="VF517_04360"
     CDS             complement(5820..6419)
                     /locus_tag="VF517_04360"
                     /inference="COORDINATES: protein motif:HMM:NF027589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEX8052202.1"
                     /translation="MFVLTLDQRASRATPDLVRAEANSLNDQFSSGLRLPFVRTAGDE
                     MQALVSDPADLVDLVLRATRSEQWWAGVGIGAIDDIGETARDSRGPAFVSARAAVEQA
                     KKNAWGCSVQGEPEALATALEDSLALLVFLRASRTSRAWELVDMATGEGMRAIDIARE
                     LGISKQAVSKQLRAAGFAEEQRGRGLAIALLQGSWTPLS"
     gene            6638..7417
                     /locus_tag="VF517_04365"
     CDS             6638..7417
                     /locus_tag="VF517_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052203.1"
                     /translation="MGRLEHVDLSSRLGKDEYAERLEAAQRRLATLRLQLAGLIGDGR
                     LGPPVLALFEGWDASGKGGAIKRLTEELDHRHVRVAAFSAPTHDEKRHHFMWRFWPQL
                     PGWGGVAVFDRSWYGRVLVERIEGFATEEQWRRAYEEIVSTERTLAAEGMILMKFWLH
                     VSEEEQLKRFEARRDDPLKRWKLTDEDWRNREQRAAYEEAIEEMFERTDHSAGPWHIV
                     PGDSKKYARVHVVETVVAEVERGMRERDFEPIDVDALKAAG"
     gene            complement(7437..8057)
                     /locus_tag="VF517_04370"
     CDS             complement(7437..8057)
                     /locus_tag="VF517_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052204.1"
                     /translation="MAAEERFWTRRLRWRLIGAWRWPAFAAAILLDAVILHELPPLGS
                     LETEPGNLNWPVSFIVSGFGNLALIALSDALGRFAESKRRAQGIHDPHIEVQSDRLAV
                     GALAVGVVGVLAFGLATQGTAVTETEATQKNAQAVRDYVLTHGSEEYKRNLQTANTER
                     LAEGYFRTCVADNSRRRFLCVFVDTKKDPPEVVRDPSMLPNQNRTP"
     gene            8208..9278
                     /locus_tag="VF517_04375"
     CDS             8208..9278
                     /locus_tag="VF517_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX8052205.1"
                     /translation="MATTTALPAAFELGVEPAGALLTPDDRARLLRFMLLMRSTEERA
                     LTLYRQGKVPGSFYDGRGQEAVSVGTAYALGPRDRACILHRDLGAHLIRGVRPGRVLA
                     QMMGRAGGITRGRDGNMHFGDRELGCVGMVSMLPDMALVACGLALSFQMRREPRVAMT
                     WFGEGSTANGVWHEAMNVAGVRRLPVVFVLENNQFAYSTPNDREFAVDPVARAAGYGF
                     PGVKVDGNDVEAVFEAAAEACSRARSGAGPTLIECETMRMHGHGAHDDMRYVPPELLE
                     QWAGRDPIETYTARLRADGIDVDAVSAAVRDELELETEWALAQPMPEPASAVEGVFAD
                     EAVPLGDGAAPWSRWETEARHA"
     gene            9271..10239
                     /locus_tag="VF517_04380"
     CDS             9271..10239
                     /locus_tag="VF517_04380"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX8052206.1"
                     /translation="MPELTYLQAISDALREEMRADDSVFCLGEDIGAFGGAFKVTDGF
                     MEEFGADRVWDTPLAEAAIVGCAVGAAVEGMRPVCEMQFADFVACGFDQLVNVAAKMH
                     YRQGLAVPLTLRLPSGGGFSGGPFHSQNPEAWFLQAPGLKVVAPSTPEDAKGLLAAAI
                     RDPNPVVYLEHKHLYRRAKGEVPAGRYELPLGEARVAREGSDLTVIAYGAMMPVALEA
                     TEDVDGASVEVIDLRSLVPLDGEAVLRSVRKTSKVVILHEATRSCGVGAEVAALIAEH
                     AFEYLDGPVVRVTAPDVPIPFSPPLERAVLPQVDDVREACRELLAY"
     gene            10220..11524
                     /locus_tag="VF517_04385"
     CDS             10220..11524
                     /locus_tag="VF517_04385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX8052207.1"
                     /translation="MSSSPTETIVEVTMPQMGVSVAEGTVVEWRKRVGEWVEADEVIV
                     EISTDKVETEVPSPTSGRVASIAVEVGETVDVGTVLATIEVDGREPGAAGAGAGRAPE
                     SAHTHTLPHSNEILESRSSGNGGACEPEAVCEPEAAGEPEAAASNGNGHAPPVSPVVR
                     RMADAHGLDLAQISGSGRRGRVTKRDVLAFLAGAEPAPAAEPVLHSESPYREEEPPAA
                     PPPGEPLSLMRRQIGEHMVRSLRTAAHCTTVVEADMQRVEEARGRLSYLPFVARATIA
                     ALREHPRLNATLAGERLTIHDEVHLGIAVALEPDGLIVPVVHNAHELSHEGLAARIAD
                     LAERARAKRLTPDETRGGTFTITNPGRYGALLATPIINQPQVAILDLEAVVRRPVVVG
                     GDSIAIRPMTYLCMSWDHRALDGVLAARFLGSVRRRLEEWDG"
     gene            11517..12206
                     /gene="lipB"
                     /locus_tag="VF517_04390"
     CDS             11517..12206
                     /gene="lipB"
                     /locus_tag="VF517_04390"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX8052208.1"
                     /translation="MAELWVANLGRRPYAEAVAIQDRVRSARQSGAIPDSLLLLEHDP
                     VYTKGRRTAASDLPMGDEWYRAQGIEVAETTRGGRVTYHGPGQLVGYPIVAIGDVIAY
                     VRTLEVAVIAALADEGVEAGTREGLTGIWVGERKIGSIGVHVSRGVTMHGFAVNVDCD
                     LQPFEWIVPCGIEGVRMTSLLAQTGRTGAMSCFRKRMAWRFAEAFGLRQRIVTADRLL
                     EASADREPVPA"
     gene            12203..13144
                     /gene="lipA"
                     /locus_tag="VF517_04395"
     CDS             12203..13144
                     /gene="lipA"
                     /locus_tag="VF517_04395"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX8052209.1"
                     /translation="MSERSTRSRSNPGGARVLDMGEVRPFRERKPPWFKVRAPGGPKF
                     MELKGMIDGQGLNTVCEEAACPNIGDCWDAGTATFMILGDTCTRRCGFCNVRTGKPDA
                     PDPLEPLRVAQSVKRMGLRHAVITSVDRDDLPDLGSIAFVGVIRSIRRFAPGCKVEVL
                     TPDFRGQEMPLARVIAERPDVFNHNVETVPRLYPLARRGSNFLRSCRVLANAKEMGGD
                     AVVTKSGLMTGLGETKEELIETFGVLREHGVQVLTVGQYLRPTREHLPVVRYWHPDEF
                     AELEREAYAMGFDSVAAGPLVRSSYHAEQTLAQASSQ"
     gene            13141..14097
                     /locus_tag="VF517_04400"
     CDS             13141..14097
                     /locus_tag="VF517_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX8052210.1"
                     /translation="MRPCLALLVAALAVTGCGEAQREPAATQRWKPLARSKLSRTEVA
                     AARIGRSIYVVGGFDQRSGGRTVAAVERYSIASDSWTRMRSMPVALNHAAAAAWNGRL
                     YVVGGYGSATGLDDEQRVLLRYDPKRHAWKRLAKPPSARAALAAAVVGDRLYAVGGVR
                     DGHPLATLEVYDLRTGRWSEGPDMGVAREHLAATAAGGQVYALAGRAAGAGNFATAER
                     YDPKSGAWERLPDMGKPRGGIAAAAVGGDVVVFGGEEAAGTIPQVERFEPAARAWSAL
                     PDMRTPRHGLGGAALGRRVYSIEGGPQPGLYFSTDLEVLDVP"
     gene            14113..14925
                     /locus_tag="VF517_04405"
     CDS             14113..14925
                     /locus_tag="VF517_04405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8052211.1"
                     /translation="MLPLKDNIPTDRFPVVTVVIIVLNVIMFFGFQHAEWAPGGGFAV
                     DNQNTIEYAEIPYEITHPGENCGVIGDVDDPGAKIVCGEPADLDRAGADPGGDQPSSW
                     LTLLTSMFMHGGLLHIAGNMLFLWIFGNNVEDSMGPVKFVAFYLLGGLAASAAQIGFS
                     PNEPVPTLGASGAVAAVLGAYALLYPRARVVTLVFIIIFITVIELPAALVLFGWAVLQ
                     VLSASSEAAVGGGGGGVAYWAHIGGFAFGLALIKLFATKQKEDRVQPRLPVY"
     gene            14925..15101
                     /locus_tag="VF517_04410"
     CDS             14925..15101
                     /locus_tag="VF517_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052212.1"
                     /translation="MGRNLALILSLAFIGMLAYLTIHVAITDGVTFLVVVSILLLAVL
                     GFGVLGALTHPSDD"
     gene            15121..16092
                     /locus_tag="VF517_04415"
     CDS             15121..16092
                     /locus_tag="VF517_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052213.1"
                     /translation="MRRRRRRPLAILVLVLLAVGAASAGIVVGHEGDNGRSRDRASVG
                     QSQWRARLGNDEREAAVRAGPPGIEFDGDTQVHVRFKRPPRAGLLLDVDSGQVLWSRK
                     PLTSVPIASLTKIMTALLVVDRIGPRAKTRISKAATKFRGSGVGVLPRGKKVPIEALL
                     YGMLLPSGNDAAVALAEAVAGSDRRFAKLMTRHAQELGLGCTRYVDSYGLRAGNQSCA
                     ADLAALTRLAIHQPRIARIVRQAHASIPFPIKGRRLDLRSTNPLLREPRYPGTIGLKT
                     GYTDRAGHCLIAVAHRGDHTLAAIVLNSPNTRDQTRRMLDTGFRALL"
     gene            complement(16093..16686)
                     /locus_tag="VF517_04420"
     CDS             complement(16093..16686)
                     /locus_tag="VF517_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8052214.1"
                     /translation="MRRRVAIAMSLLALAAAAAAEAKSIGGTARADRIAGTNAADRIV
                     GRGGDDKIDGRRGNDKIFGNAAADRLSGSEGNDQIRGGGENDAVLGGNDDDVLRGEAG
                     DDKVVGGDRDDELDGGLGNDRLTGGPGFDLVQGGEGDDRVNGGLGEDDLFGGPGNDTI
                     QARDSGDDTIDCGDGEDTVIVDDFEDGVFDCETVVLP"
     gene            complement(16683..18161)
                     /locus_tag="VF517_04425"
     CDS             complement(16683..18161)
                     /locus_tag="VF517_04425"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8052215.1"
                     /translation="MSDIRDPEYAEQLAHESPDEYLRRRDFLQRTAATAGLAAGMATA
                     LNPNALVAEAARRQRRKPLPSPRNLPIDTFVVLMMENRSFDHYLGWLPGADGRQAGLT
                     YLDDDGVPHATRRWAPDFQGCDNVDPSHSWNGGREQFNGGRMDGFRRAAGNDDFALSY
                     YEKDDLPFISHAAVASTTYDRFFCSLLGPTFPNREYMHAAQSYGKKDNSLPVDEGGFP
                     DTTIFAALAARGLTGRCYFNDLPAAGLWGAPGLARSARVEEYYARAASGTLPNLSFVD
                     PFFGGNDPASAPGVRGDEHPHGDIRAGQAFMSDVAHAFMESPQWSRGALFLVYDEWGG
                     FFDHVRPPRVPDDRNSRNLAEDYGRMGMRIPAVVLSPYARTGHVEHGTYGFESILKMI
                     EYRFGLGPLTRRDRYAANIARSFDWESKPREDPPELPSPEQIASAPCSNEGPAGAAPD
                     HELMKLHTSGFLERLGFDYRPATSASTFRYPDSFERAYFGTA"
BASE COUNT         2650 a         6323 c         6737 g         2483 t
ORIGIN      
        1 gacgagttcg gggagcggcg ggtgcgtgac acgccgatct ccgagaacac gatcgtcggg
       61 atgggggtcg gagcggcgat ggccgggctg cgaccggtcg tggagatcat gaccgtcaac
      121 ttcgccctcc tcgcgatgga ccagatcgtg aactgcgcgg cgtcgatccg ctacatgttc
      181 ggcggccagg tgacggtgcc gatggtgatc cggatgccgc agggggcggg gcaccagctc
      241 ggtccgaccc actcacactc gttcgaggcc ctgtacctgc acgtgccggg cctgctggtg
      301 gccgtcccgt ccacgcccga ggacgcgcgg gggctcctga aggccgcgat ccgcgacgac
      361 aacccggtga tcttcatcga gcacgagacg ctctacggca agaagggcga ggtgcccgac
      421 gaggccgagc ccatgcgctt cggcgaggcc gccatccgcc gcgagggcga ggacgtgacg
      481 atcgtcggga tctcgcgcat ggcgcacacc gcggccgacg cggccgagac gctcgcggag
      541 gagcacgaga tcgaggccga ggtgatcgac ccgcgcacgc tgcgcccgct cgacctcgac
      601 acgattctcg agtccgtgcg caagaccaac cgcgcggtga tcgtcgagga gggctggccg
      661 cacggcggcg tgggcgcgaa cctcgcggcg ctgatccagg agcaggcgtt cgaccacctc
      721 gacgcgccga tcgagcgcgt gaccggcgcc gacgtgccga tgccgtactc gaagccgctc
      781 gagcaggccg cgttcccgca ctccgagcac gtggtgcagg cggcactcgc gacgttcagg
      841 gacgtttgac ggagcccgct cgggtcgtgg cgacccgccg tccgcgcccg tcccggccgt
      901 cgaacagcgc ctgaaggcga agctgacgcg ggcgagcggc gagccgccgc cggtctgcgg
      961 ctgagggcat cagggtgatg cgccgtacgc gagtgctcgc gcgcaggtcg agggaacgga
     1021 ccgcgagcgg cgccgtggcc cccagcgccg cgtccgcgtc cgcccgcagt cgtccgcgac
     1081 agccgcgacg gcacaccacc ttgaccacga ccgcctcgcc ggatccaagt ccgatcccct
     1141 gtatgcgggg tggtgtgcag ggcggtggag ccggctcgcc gcgcggaggg tcgaccgagt
     1201 ccacgtcggg atcgaccagc acggagacga acccgcagcc ggatgcggtc catgtggcgc
     1261 gccgtccgtc gaagtcgaac ccggaagctc tggcgggagc gccggccacg tcatgcccct
     1321 ggccgtcgag atccagcacg gcgagccccg cgccgccgcc ggactcgacc gcgcgcgtgg
     1381 cgagtatccg gtccccggcg atccgcgcgc tcaggtagcg ctcccctggg ggcggctcga
     1441 tcgcgtgtgc tcgcggctcc gccggactga accagacgag ccgtccctgg gagtcgacgt
     1501 cgacgagctt gccgtcggcc tgcagctccg gagacgagta gccggagcgg cggtagacct
     1561 cgcgccgcgc gcgccagtct tacacgacgg tgtgcgagcc caggttggag aagcccacgt
     1621 acgcgacgtg gtcgccggcg atgcggatgt ggtcgccggc gtcgccgccg acctcgatac
     1681 ggaccggcgc atccgccgag gcgaggtcgt ccacgacgat cgtgcggtgg gggtagttgc
     1741 cctccacgta ggcgagcacg tcgccgtcga ggtccgccgc gtacgagaag ttgctgcaga
     1801 actggggaca ccggcactcg ctgggcccgt cgccacagcc ggtgacgatc gaggcgccgc
     1861 cggcgccggt cgcgtacagg cggctgcgaa tggaccggta cgacatcccg tgcgcgtcca
     1921 gcacgtcgaa ggcggtgtcc gtccacgcga cccggctcgc cgatccgccc gcgggccgga
     1981 ccgtgtggat cacccggctt ccgatggtcg gtcgctcgct cgtcgccagc cgcgtgaccg
     2041 gctggccggg cgccgcacgc tccacgaccc agccgccctg cggacggctg cgcgcccagg
     2101 cggccgaccc acccgcgagc accggtccgc tccagctgta gaacgaattg gactccgacg
     2161 cgtacggctc cgcgtaggac gggacgaagg tcgacacgtc acgcggcgcg gcgccggccg
     2221 gggacggagg ggccgcgaat gccgccagca cggtggcgat cgctgcggct gcggcgggcc
     2281 agctacgggc catgcttcag ctaacccgcg ccaaacgcaa gcgatgcgcc cgcgggtggt
     2341 gacagaatca ccgacgtggc caccgacgtc accatgccgc ggctgtccga ctcgatggag
     2401 gagggcacga tcctcaagtg gctcgtcgag cagggcggcg aggtgaagaa gggcgagccg
     2461 ctcgtcgaga tcgagaccga caaggcgaac atgacctacg acgccgatac gagcggcgtg
     2521 ctgatcgagg tggtcgcagg cgagggcgac acgctgccga tcggcgaggt gatcgcgcgg
     2581 atcggcgagg cgggcgagaa gccggcggag gactccgggg attcgggtgc ggacgaccag
     2641 gaggagccgg aggaggccga ggagccggag gaggccgagg agccgaagga gaagccgaag
     2701 cgcgagccgc agccacaggc gtcgggcgcg agcaacccgg tcggcggtcc gggcggtgga
     2761 ggaggcggca acggggggtc cgccgacggc gagcgcgtga aggcctcgcc cgtggcgcgc
     2821 cgggtggcga aggatctcgg tatcgagctc gcgcagctcg agggcacggg acccggtggg
     2881 cggatcgtga aggcggacgt cgaggccgcc gcgcagggga atggaaagcc gcaggacgag
     2941 gctccgccgg cggaagccga gaaggaggag gcccccgccg aggctccggc ccccaaggcc
     3001 gacgccggcg ccaagggtga ggtcgagacg caggagctgt cgcggctcca gaagaccgtc
     3061 tcgcggcgga tggccgagtc gaaggccacg gcgcccgact acacgctgca catgcagatc
     3121 gacatgacgc tgtgcgtcga gctgcgcaag cgcctcaagg aagtcgagga cccggcgccg
     3181 tcgttcaacg acatgatcgt caaggcgtgc gcgaacgccc tgcgcgagca cccgcgcgtg
     3241 aacggcgcat accgcgacgg acgcttcgag ctgtacggga acgtcaacgt cggcgtggcg
     3301 gtggccgcga gcgacgcgct ggtggtgccg accatcttcg aggccgacaa gaagtcgctc
     3361 ggcgagatcg cgcgtcaggc gcgggcgctg attgagaagg tgcgtgacgg aaagatcacg
     3421 cccccggagg tctccggcgg caccttcacg gtgtcgaacc taggcatgta cggcgtcgac
     3481 gagttctcgg ccgtgatcaa cccgccgcag gccgcgatcc tcaccgtcgg ctcgctcaag
     3541 aagcggccgg tcgtggacgg cgacggccgc gtggtcgcgc gcgacacgct caccctcagc
     3601 atggtgagcg accaccggat cctctacggg gccgacgccg cgcagttcct cgcccgcgtc
     3661 cgcgagctgc tcgagcagcc gctcgcgctc gcgctctagt gctgcgcgcg ctcgtcctcg
     3721 tcctcagcgc cgccgccctg ctggccggct gcgacgacga gggagacggg gtcgtcttca
     3781 aggatccgaa gggcgagatc gccgtggagg agggcatgga gttcaccctc gagctccggg
     3841 tcaacgcgag cgtgggctac gactgggtcc cggtcgcgcc cggccggagc gggccggtga
     3901 ggctgaaggc gacgagcgtc gactacccga acgaggagag catcggcgac tcgggcttca
     3961 agcgcttcac ctacgaggcg acccgcacgg gtcaggggac gatcgagttg cgcaggctgt
     4021 tccgcgggga cccgcaggag cgccggctgc tgatcgtgcg cgtgcggggc tagccccagc
     4081 ggaagccggc cggcgcgatc tgcggctcga gcgggctgac cggcaggccc ggctccgggt
     4141 cgggcaggtt gaagcccgag ggtgcaacgt cgttcggcgt gtcggggtgg aagagcgagg
     4201 ccagatactg gatgtggccg cgccccgcgc cgagctcgaa ctgcccgccg ccgtaggcgc
     4261 cgatgccgtg ctcggcgcag tagtcgtagg tcgcgcagac atccgcgacc ccgccgacgc
     4321 gcgagggctt gatgttcacc atccgcgggg cgaacggcag cgactcgatg tcggcgatcg
     4381 agtggatcgg cgcgtcccag gtgatgcggt cgcggtaggg ctcgagcacc ggatcggtct
     4441 cgtcggtgag cgcgggatcc tcgatccatg cgtccgggaa cgcctcggcg acccgtcggt
     4501 acaggtcggg gtcggccgcc tggtcgacgg gagtgccgac gtagtagccc ttgaagtcga
     4561 ccgagtcgac agcgccggtg gcaaccagct cctggatcag ctcctcgctc cagtcgctgg
     4621 tcgggtcgag cttgaagcgg agcgtcgggt agctcgcgag gcggctggtg atcggctcga
     4681 gcgtgggcgg atcgccgagc cgcagcgaga cgacgaacgt gaccggacgc ggctcgagcc
     4741 cgagccgcgc ggccagcgac tcgccggcct ggcgcagcgc caggtcgagc gccgccgact
     4801 cgaaggccca gcggcggtag agccgcgaga cgtcgcggac gggcggcgag tcctcgaaca
     4861 ggtcgagcga gccggtcagc tcgcaaagct cgccgaacgt ccgcggcccc gtcaggtcgt
     4921 ggacgggccc ggtggcctgg aacgcgatgt ggtcgagcgc gtcgtacacg acgtcctcgc
     4981 caacgccctc ctcgccgccg ccactcatcc ggaccagcgt gctgagccgg gtgaaggagc
     5041 tcgacacgtc ggcctcgagt ccctctaggg cgtagccgtc gatctgaatc gggaggtccg
     5101 cgaggcggtc gaaggtgggc atgaacgcgg agattatgcg agtacaggca tgaggccgag
     5161 gatcgcacgc acggcgaccc cgagcccgac ggcaacaacc acgctcgcaa gcgtgccgat
     5221 caggaagtac tcggcgaact tctcctccgt gaacgacggg aagcgcgcga tcgacttgac
     5281 cgcgaccact acggccacgg caccgggcgc gccggcgacc acgaacccgt agatcagcgt
     5341 ccgctcgagc cagccgatgt agaggcccgc gcgagtgggg gtcacgacac ccgcgtcggc
     5401 cgcggtctcg tgcaccagcg cggcgaacgg cgtgagcagc catgcgatcg ccgcgccgcc
     5461 gacgaacacc gtgagcaggg cgcccgccgc cacgatcgcg acctggtcat cgaggagcgc
     5521 gtcctcgatg ctctccgcgg ccgctgaccc gtcgagcagc agcgccgacg ccccagccag
     5581 gaccaccgtg cacaggacgg cgcgagcgaa cgactcgcgc acctcgccgt caatcgaccg
     5641 cgcgagctgc gccgccacga ccgccagtgc cacgagcgcc caaccgcgcc agtcgtcgag
     5701 cgttggcgcc gcagcccaca tggcaagcgg cacagcggcg agcgcgcgac ccgcgcgacc
     5761 aagccgcggg accatggccg gcacgacgcc ggcggccggc agcgcgatca ccacacgcgt
     5821 cagctcagtg gtgtccatga tccttgcagt agcgcgatcg ccaatcctcg cccccgttgc
     5881 tcctccgcga agccggcggc cctgagctgc ttgctgacgg cctgcttcga gattccgagc
     5941 tcgcgcgcaa tgtcgatggc acgcatcccc tcgccggtgg ccatgtcgac gagctcccat
     6001 gcccgcgacg ttctcgaagc cctcagaaag accagcagcg ccaggctgtc ctcgagcgcg
     6061 gtcgcgagcg cctcgggctc cccctggacc gaacagcccc acgcgttctt ctttgcctgc
     6121 tcgacggccg cccgcgcgct cacgaaggcc gggccgcgcg agtcgcgcgc tgtctcgccg
     6181 atgtcgtcga tcgccccaat tcccacgccc gcccaccact gctccgaacg tgtggcgcgg
     6241 agcaccaggt ccacgaggtc ggcgggatcg ctcacgagcg cctgcatctc gtcaccggcc
     6301 gtgcggacga aaggaaggcg caggccggag gagaactgat cgttgagcga gttcgcctcg
     6361 gcccgcacca ggtcgggtgt cgcgcggctg gcacgctggt ccagggtcag cacgaacatc
     6421 gtgcgtcaac cttatgcggt tgactaccga tagtcaaccg ttcacggttg acttcagggc
     6481 ttcgctgtca accgggaccg accggcgcac cgccggtgaa gtcaacccaa ctaggttgtc
     6541 tgtcatcccc tggcggttga cttcaagaag tcaaccgata tgggttgacc agtcgtgcgc
     6601 gtggcccaag acgctcgaga gtgagaggat tcggcccgtg gggaggctcg agcacgtcga
     6661 cctgtcgagc aggctcggca aggacgagta cgcggagcgg ctcgaggcgg cgcagcgccg
     6721 gctcgccacc ctgcggctcc agctcgccgg cctgatcggc gacgggcggc tcgggccgcc
     6781 ggtgctcgcg ctgttcgagg gctgggacgc gtctggcaag ggcggcgcca tcaagcgcct
     6841 cacggaggag ctcgaccacc gccacgtgcg cgtggccgcg ttctccgccc cgacccacga
     6901 cgagaagcgc caccacttca tgtggcgctt ctggccgcag ctcccggggt ggggcggcgt
     6961 ggccgtcttc gaccgctcct ggtacggccg cgtcctggtc gagcggatcg agggcttcgc
     7021 gaccgaggag cagtggcggc gcgcctacga ggagatcgtc tcgaccgagc gcacgctcgc
     7081 ggccgagggc atgatcctca tgaagttctg gctgcacgtc tccgaggagg agcagctcaa
     7141 gcgcttcgag gcccgccgcg acgacccact caagcgctgg aagctgaccg acgaggactg
     7201 gcgcaaccgc gaacagcgcg ccgcctacga ggaggccatc gaggagatgt tcgagcgcac
     7261 cgaccactcc gccggcccgt ggcacatcgt ccccggcgac tcgaagaagt acgcgcgcgt
     7321 gcacgtggtc gagacggtcg tcgccgaggt cgagcgcggg atgcgggagc gcgacttcga
     7381 gccgatcgac gtcgacgcgc tgaaggccgc cggctgaacg cgccgccgcc gcccgctcag
     7441 ggcgtgcggt tttggttcgg cagcatgctc gggtcgcgca cgacctcggg cgggtccttc
     7501 ttcgtgtcga cgaagacgca caggaagcgc cggcgcgagt tgtcggccac acacgtcctg
     7561 aagtagccct ccgccagccg ttcggtgttg gcggtctgga ggttgcgctt gtactcctcc
     7621 gagccgtgcg tgaggacgta gtcgcgcacc gcctgggcgt tcttctgggt cgcctccgtc
     7681 tccgtcaccg cggtgccctg ggtggcgaga ccgaaggcga gcaccccgac gactccgacg
     7741 gcgagcgccc ccaccgccag ccggtccgac tggacctcga tgtgcgggtc gtggatcccc
     7801 tgcgcgcggc gcttgctctc ggcgaagcgt ccgagcgcgt ccgacagcgc gatcagcgcg
     7861 aggttgccga agccggagac gatgaacgac accggccagt tgaggttgcc gggctcggtc
     7921 tccagcgagc cgagcggcgg cagctcgtgg aggatcaccg cgtcgagcag gatcgccgcc
     7981 gcgaaggcgg gccagcgcca cgcgccgatc agtcgccagc gcaggcgccg cgtccagaac
     8041 cgctcctcgg ctgccacagg cggagggtag acagacggtg gccgagcggc cggatgcagg
     8101 agtaccatcg ctcgagacac gcgttgcccg cgcggagacc ccgcagggcg cgcaaccgat
     8161 tcgagacccg cgcggagacc ccgacgggct cgagggagga gacatagatg gcgacgacga
     8221 ccgcgctacc cgcggccttc gaactcggcg tcgaaccggc aggggccctc ttgacgcccg
     8281 acgacagggc tcgcctcctg cggttcatgc tgctgatgcg ctcgaccgag gagcgcgcgc
     8341 tcacgctcta ccggcagggc aaggtgccgg gctcgttcta cgacggacgc ggccaggagg
     8401 ccgtgtcggt cgggacggcg tacgcgctcg gtccacgcga ccgcgcgtgc atcctccacc
     8461 gcgacctcgg cgcccacctg atccgcgggg tccgtcccgg acgagtgctc gcccagatga
     8521 tgggacgcgc cggcggcatc acccgcggcc gggacggcaa catgcacttc ggcgatcgcg
     8581 agctgggctg cgtggggatg gtctcgatgc tgccggacat ggcgctggtc gcgtgcgggc
     8641 tggccctgtc gttccagatg cggcgcgagc cgcgcgtggc gatgacctgg ttcggcgagg
     8701 gctcgaccgc caacggcgtg tggcacgagg cgatgaacgt cgccggcgtg cggcggctgc
     8761 cggtcgtttt cgtgctcgag aacaaccagt tcgcgtactc gacgccgaac gaccgcgagt
     8821 tcgcagtcga tccggtcgcg cgcgcggcgg gctacgggtt cccgggcgtg aaggtcgacg
     8881 gcaacgacgt cgaggcggtc ttcgaggccg cggccgaggc gtgctcgcgc gcgcggtccg
     8941 gcgccggccc gacgctgatc gaatgcgaga ccatgcgcat gcacggccac ggcgcgcacg
     9001 acgacatgcg ctacgtgccg ccggagctgc tcgagcagtg ggccgggcgc gacccgatcg
     9061 agacctacac ggcccggctg cgggccgacg ggatcgacgt ggacgccgtg tcggcggccg
     9121 tgcgcgacga gctcgaactt gagaccgagt gggcgctcgc gcagccgatg ccggaacccg
     9181 cgtccgcggt cgagggcgtg ttcgcggacg aggctgtccc gctgggcgac ggcgccgcgc
     9241 cgtggtcgcg ctgggagacg gaggctcgcc atgcctgagc tcacctacct ccaggcgatc
     9301 tccgacgcgc tccgggagga gatgcgagcc gacgactcgg tgttctgcct cggcgaggac
     9361 atcggggcgt tcggcggcgc gttcaaggtc accgacggct tcatggagga gttcggggcc
     9421 gaccgcgtgt gggacacgcc gctggcggag gccgcgatcg tgggctgcgc ggtgggcgcc
     9481 gccgttgagg ggatgcggcc cgtctgcgag atgcagttcg cggacttcgt cgcgtgcgga
     9541 ttcgaccagc tcgtgaacgt ggcggcgaag atgcattacc gccaggggct cgcggtccca
     9601 ctgacgctgc gcctgccgtc cggcggcggc ttctccggcg ggccgttcca ctcgcagaac
     9661 ccggaggcgt ggttcctcca ggcacccggg ctgaaggtgg tcgcgccgtc gacgccggag
     9721 gacgccaagg gcctgctcgc ggctgcgatc cgggacccga acccggtcgt ctacctcgag
     9781 cacaagcatc tctacaggcg cgcgaagggc gaggttccgg cgggtcggta cgagctgccg
     9841 ctgggcgagg cgcgggtggc gcgcgagggc agcgacctga ccgtgatcgc ctacggcgcg
     9901 atgatgcccg tggctctcga ggcaaccgag gacgtcgatg gcgcgagcgt ggaggtgatc
     9961 gacctgcgct cgctcgtgcc gctcgacggc gaggcggtgc tgcgctcggt gcgcaagacc
    10021 agcaaggtcg tgatcctgca cgaggccacg cgctcgtgcg gggtcggggc ggaggtggcc
    10081 gcgctgatcg ccgagcacgc cttcgagtac ctcgacggcc cggtggtgcg ggtgaccgcg
    10141 ccggatgtgc cgatcccgtt cagcccgccg ctcgagcgcg cggtgctgcc ccaggtcgac
    10201 gatgtgaggg aggcgtgccg tgagctcctc gcctactgag acgatcgtcg aggtcacgat
    10261 gccgcagatg ggcgtctccg tcgccgaggg gaccgtggtc gagtggcgca agcgcgtcgg
    10321 cgagtgggtc gaggcggacg aggtgatcgt cgagatctcg accgacaagg tggagaccga
    10381 ggtgccctcg ccaacatcgg gccgggtcgc gtcgatcgcg gtggaggtcg gcgagacggt
    10441 ggacgtgggg acggtgctcg cgacgatcga ggtggatggg cgcgagccgg gggccgcggg
    10501 cgcgggggcg ggccgggcgc cggagtcggc ccacacgcac accctcccgc actcgaacga
    10561 aattctagaa tctcgttcta gtggtaacgg gggtgcgtgc gagcccgagg cggtgtgcga
    10621 gcccgaggcg gcgggcgagc cggaggcggc ggcctcgaac ggcaacggtc acgcgccgcc
    10681 ggtctcgccg gtggtccggc ggatggccga cgcgcacggg ctcgacctgg cgcagataag
    10741 cgggagcggt cggcgcgggc gggtcacgaa gcgggacgtg ctcgcgttcc tggcgggggc
    10801 ggagcccgcc cccgcggcgg agcccgtgct ccattcggag tcgccctacc gggaggagga
    10861 gccaccggcg gctcccccgc ccggcgagcc gctgtcgctc atgcggcgcc agatcggcga
    10921 acacatggtc cgctcgctcc ggaccgcggc gcactgcacc acggtcgtcg aggccgacat
    10981 gcaacgcgtc gaggaggctc gggggaggct ctcgtacctc ccgttcgtcg cccgcgcgac
    11041 gatcgcggcc ctgcgcgagc atccgcgtct gaacgccacc ctcgcgggcg agcggctgac
    11101 gatccacgac gaggtccacc tcgggatcgc ggtcgcactg gaacccgacg ggctgatcgt
    11161 tccggtcgtg cacaacgccc acgagctctc gcacgagggc ctcgcggccc gcatcgcgga
    11221 cctcgccgag cgcgcgcggg cgaagcggct cacccccgac gagacccgcg gcgggacgtt
    11281 cacgatcacc aaccccggtc gttacggcgc gctgctcgcg accccgatca tcaaccagcc
    11341 gcaggtcgcg atcctcgacc tcgaggcggt cgtccgccgg ccggtcgtgg tcggtggaga
    11401 tagcatcgca atcaggccga tgacgtacct ctgcatgtcc tgggaccacc gcgcgctcga
    11461 cggagtgctg gcggcgcgat tcctcggcag cgtgcgccgt cgcctggagg agtgggatgg
    11521 ctgagctgtg ggtggcgaac ctcggccgcc gcccgtacgc ggaggccgtc gcgatccagg
    11581 accgggtccg ctcggcgcgc cagtccggcg cgattcccga ctcgctgctg ctgctcgagc
    11641 acgaccccgt ctacacgaag ggccgccgga cggccgcaag cgacctcccg atgggcgacg
    11701 agtggtaccg cgcgcagggg atcgaggtgg ccgagaccac ccgcggcggc cgcgtcacct
    11761 accacggccc gggccagctc gtgggctacc cgatcgtggc gatcggcgac gtgatcgcct
    11821 acgtgcgcac gctcgaggtc gcggtgatcg ccgcgctcgc cgacgagggc gtcgaggcgg
    11881 gcacccgcga ggggctgacc gggatctggg tcggcgagcg caagatcggc tcgatcggcg
    11941 ttcacgtctc gcgcggcgtg acgatgcacg gcttcgcggt caacgtggac tgcgacctcc
    12001 agccgttcga gtggatcgtc ccgtgcggga tcgagggagt gcggatgacc tcgctgctcg
    12061 cgcagaccgg ccgcaccggc gcgatgagct gcttccgcaa gcggatggcg tggcgcttcg
    12121 ccgaggcgtt cgggctgcgc cagcggatcg tgacggcgga tcgcctgctg gaggcgagcg
    12181 cggaccgcga gccggtgccg gcatgagcga gcgctccacc cggtcgcgct ccaaccccgg
    12241 cggcgcgcgc gtgctcgaca tgggcgaggt ccgtccgttc cgcgagcgca agccgccgtg
    12301 gttcaaggtc cgtgccccag gcggcccgaa gttcatggag ctgaagggga tgatcgacgg
    12361 ccaggggctc aacacggtct gcgaggaggc ggcctgcccg aacatcggcg actgctggga
    12421 cgccggcacg gcgacgttca tgatcctcgg cgacacctgc acccgccgct gcgggttctg
    12481 caacgtccgg accggcaagc cggacgcgcc cgacccgctc gagcccctgc gcgtcgcgca
    12541 gtcggtcaag cgcatgggcc tgcgccacgc ggttatcacg agcgtcgacc gtgacgacct
    12601 ccccgacctc ggttcgatcg cgttcgtcgg cgtgatccgc tcgatccgcc gcttcgcgcc
    12661 aggctgcaag gtcgaggtgc tcacccccga cttccgcggt caggagatgc cgctcgcccg
    12721 tgtcatcgcc gagcgcccgg acgtgttcaa ccacaacgtc gagaccgtgc cgcggctcta
    12781 cccgctcgcg cggcgcggct ccaacttcct gcgctcgtgc cgcgtgctcg cgaacgcgaa
    12841 ggagatgggc ggcgacgcgg tcgtgacgaa gtccgggttg atgaccggcc tcggcgagac
    12901 caaggaggag ctgatcgaga ccttcggcgt gctccgcgag cacggcgtgc aggtgctcac
    12961 ggtcggccag tacctgcgcc cgacgcgcga gcacctgccg gtcgtgcgct actggcaccc
    13021 ggacgagttc gccgagctcg agcgcgaggc ctacgcgatg ggcttcgact cggtcgcggc
    13081 gggcccgctc gtccgctcga gctaccacgc cgaacagacg ctggcgcagg cctcgtccca
    13141 atgagacctt gccttgccct cctggtcgcc gcgcttgcgg tgaccgggtg cggggaggca
    13201 caacgcgagc cggccgccac gcagcgctgg aagccgctcg cgcggtcgaa gctctcgcgc
    13261 accgaggtcg ccgccgcccg catcggccgc tcgatctacg tggtcggcgg cttcgaccag
    13321 aggagcggcg ggcgcactgt cgcggcggtc gagcgctaca gcatcgcgag cgacagctgg
    13381 acgcgcatgc gctcgatgcc ggtcgcgctc aaccacgcgg ccgcggcggc gtggaacgga
    13441 cgcctctacg tggtgggcgg ctacggctcg gccaccgggc tcgacgacga gcagcgcgta
    13501 ctgctccgct acgaccccaa gcgccacgcc tggaagcggc tcgccaagcc gccgtcggcc
    13561 cgcgccgccc tcgccgcggc cgtcgtcggc gaccggctct acgcggtcgg cggcgtgcgc
    13621 gacggccacc cgctcgcgac gctcgaggtc tacgacctcc gcacgggccg ctggtcagag
    13681 ggcccggaca tgggcgtcgc gcgcgagcac ctggcggcga ccgcggcagg tggccaggtc
    13741 tacgcgctcg ccgggcgcgc cgccggcgcg ggcaacttcg ccaccgccga gcgctacgac
    13801 ccgaagtcgg gcgcctggga gcggctgccg gacatgggca agccgcgcgg cgggatcgcc
    13861 gccgcggcgg tgggcggtga cgtggtcgtc ttcggcggcg aggaggccgc gggcacgatc
    13921 ccccaggtcg agcgcttcga gcccgccgcg cgcgcctggt ccgctctgcc cgacatgcgc
    13981 acaccgcgcc acggcctcgg cggcgccgcc ctgggccggc gcgtctactc gatcgagggc
    14041 gggccgcagc cgggtctcta cttctcgacc gacctcgagg ttctcgacgt tccctaacct
    14101 gaagcggccg ccatgctgcc gctgaaggac aacatcccga ccgaccgctt cccggtggtc
    14161 acggtcgtga tcatcgtcct caacgtgatc atgttcttcg gcttccagca cgccgagtgg
    14221 gcaccgggcg gcgggttcgc cgtcgacaac cagaacacga tcgagtacgc cgaaattccg
    14281 tacgagatca cccacccggg cgagaactgc ggcgtgatcg gcgacgtgga cgatccgggg
    14341 gccaagatcg tctgcggcga gcccgcagac ctcgatcgcg cgggggccga cccgggcggc
    14401 gaccagccct cgagctggct cacgctgctc acctcgatgt tcatgcacgg tgggctgctc
    14461 cacatcgcgg ggaacatgct gttcctctgg atcttcggga acaacgtcga ggactcgatg
    14521 ggcccggtca aattcgtggc cttctacctg ctcggcgggc ttgccgcgtc ggccgcccag
    14581 atcgggttct cgccgaacga gccggtgccg acgctcggcg cgagcggcgc ggtggcggcg
    14641 gtcctcggcg cctacgccct gctctatcca cgcgcgcgcg tggtcacgct cgtcttcatc
    14701 atcatcttca tcaccgtgat cgagctgccg gcagcgctcg tcctgttcgg ctgggcggtg
    14761 ctccaggtgc tcagtgcgag ctcggaggct gcggtcggcg gtggaggcgg cggggtcgcc
    14821 tactgggccc acatcggcgg cttcgcgttc ggcctggcgc tgatcaagct gttcgcaacc
    14881 aagcagaagg aagaccgcgt ccagccgcgg ctgcccgtct actgatgggg cgcaacctcg
    14941 cgctgatcct ctcgctcgcg ttcatcggca tgctcgcgta cctgacgatc cacgtcgcga
    15001 tcaccgacgg tgtcaccttc ctcgtggtcg tctcgatcct gctgctcgcc gtcctcggct
    15061 tcggggtgct cggagcgctc acccacccct cggatgacta gcccgctcca gggtgggacg
    15121 atgcggcgac ggcgccggcg accgctggcg atcctggtac tcgtactgct cgcggtcggg
    15181 gcggcctcgg cggggatcgt ggtcgggcac gagggggaca acgggcggtc gcgcgaccgg
    15241 gcgtcggtcg ggcagtcgca gtggcgggcg cggctcggca acgacgagcg cgaggccgcc
    15301 gtgcgagccg gcccgcccgg gatcgagttc gacggcgaca cccaggtgca cgtgcgcttc
    15361 aagcgcccgc cgcgcgccgg actcctgctc gacgtcgact ccgggcaggt gctctggtcg
    15421 cgcaagccgc tcaccagcgt gccgatcgcg agcctgacga agatcatgac cgcgctgctg
    15481 gtggtggacc gaatcggccc gcgcgcgaag acgcggatca gcaaggcggc cacgaagttc
    15541 cgcgggtccg gggtcggcgt gctcccgcgc gggaagaagg tgccgatcga ggcgctgctg
    15601 tacgggatgc tgctgccgtc gggcaacgac gccgccgtgg cgctcgccga ggccgtggcg
    15661 ggctccgacc gccgcttcgc gaagctgatg acccggcatg cccaggagct cggcctcggc
    15721 tgcacgcgct acgtggactc gtacgggctg cgcgccggca accagtcgtg cgccgcggac
    15781 ctcgccgcgc tcacgcgcct ggcgatccac cagccccgca tcgcgcggat cgtccgccag
    15841 gcccacgcga gcatcccgtt cccgatcaag ggccgccggc tcgacctgcg cagcacgaac
    15901 ccgctgctgc gcgagccgcg ctacccgggc acgatcggcc tgaagaccgg ctacaccgat
    15961 cgcgccgggc actgcctgat cgcggtggcg caccgcggcg accacacgct ggccgccatc
    16021 gtgctcaact cgcccaacac ccgcgatcag acgcggcgga tgctcgacac ggggttccgc
    16081 gcgctgctct agctacggca ggacgacggt ctcgcagtcg aagacgccgt cctcgaagtc
    16141 gtcgacgatc acggtgtcct cgccgtcccc gcagtcgatc gtgtcgtcgc cggagtcgcg
    16201 cgcctggatc gtgtcgttgc cggggccgcc gaagaggtcg tcctcgccga ggccgccgtt
    16261 cacgcggtcg tcgccctcgc cgccctggac gagatcgaag ccgggcccac cggtgaggcg
    16321 gtcgttgccg agcccgccgt cgagctcgtc gtcgcggtcg ccgcccacga ccttgtcgtc
    16381 gccggcctcg ccgcgcagga cgtcgtcgtc gttgccgccg agcacggcgt cgttctcgcc
    16441 accgccgcgg atctggtcgt tgccctcgct gccgctgaga cggtcggcgg ccgcgtttcc
    16501 gaagatcttg tcgttgccgc ggcggccgtc gatcttgtcg tcgccgccgc gccccacgat
    16561 gcggtcggcg gcgttagttc cggcgatccg gtcggcccgg gcggtgccgc cgatgctctt
    16621 cgcctcggcg gcggcggccg cggcgagggc aaggagggac atggcgatgg cgactcgtcg
    16681 tctcatgcgg tcccgaagta tgcgcgttcg aagctgtcgg ggtagcggaa ggtgctcgcg
    16741 gaggtggccg ggcggtagtc gaagccgagc cgctcgagga agccggaggt gtgcagcttc
    16801 atgagctcgt ggtccggagc ggcgccggcc gggccctcgt tcgagcacgg cgccgacgcg
    16861 atctgctccg ggctcggcag ctccggaggg tcctcgcgcg gcttggactc ccagtcgaac
    16921 gagcgcgcga tgttcgctgc atagcggtcg cggcgcgtga gcggccccag gccgaagcgg
    16981 tactcgatca tcttgaggat cgattcgaag ccgtaggtcc cgtgctccac gtgaccggtg
    17041 cgcgcgtacg gcgagagcac gaccgcgggg atgcgcatgc ccatccgccc gtagtcctcg
    17101 gccaggttgc ggctgttgcg atcgtcgggc acgcgcggcg ggcgcacgtg gtcgaagaag
    17161 ccgccccact cgtcgtagac gaggaacagc gcgccgcggc tccactgcgg cgactccatg
    17221 aacgcgtggg ccacgtccga catgaacgcc tggccggccc ggatgtcgcc gtgcgggtgc
    17281 tcgtcgccgc gcacgcccgg cgcgctggcg gggtcgttgc cgccgaagaa ggggtccacg
    17341 aacgagaggt tcgggagcgt gcccgaggcg gcccgggcgt agtactcctc cacgcgcgcg
    17401 gagcgcgcga ggccgggggc gccccagagg ccggcggcgg gcaggtcgtt gaagtagcag
    17461 cggccggtca gcccgcgcgc ggcgagcgcc gcgaagatcg tcgtgtcggg gaagccgccc
    17521 tcgtccaccg gcaggctgtt gtccttcttg ccgtacgact gcgcggcgtg catgtactcg
    17581 cggttgggga aggtggggcc gagcagcgag cagaagaagc ggtcataggt ggtggaggcg
    17641 accgccgcgt gcgagatgaa cggaaggtcg tccttctcgt agtaggagag agcgaagtcg
    17701 tcgttgccgg cggcgcggcg gaagccgtcc atccggccgc cgttgaactg ctcgcggccg
    17761 ccgttccagc tatggctcgg atccacgttg tcgcagccct ggaagtcggg cgcccagcgg
    17821 cgggtcgcgt gcggcacgcc gtcgtcgtcg aggtaggtga gccccgcctg gcggccgtcc
    17881 gcacccggca gccagccgag gtagtggtcg aacgagcggt tctccatcat cagcacgacg
    17941 aacgtgtcga tcgggaggtt gcgcggcgac ggcagcggct tgcggcgctg gcgccgggcg
    18001 gcctccgcga cgagcgcgtt gggattcagg gcggtcgcca tcccggcggc gaggcccgcg
    18061 gtcgcggcgg tgcgctggag gaagtcgcgc cggcgcaggt actcgtccgg cgactcgtgg
    18121 gccagctgct cggcgtactc cgggtcgcgg atgtcgctca tggcgcgcac gctaccgagt
    18181 ctctctacgc tag
//
LOCUS       DASVPL010000094         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_206686, whole genome shotgun sequence.
ACCESSION   DASVPL010000094 DASVPL010000000
VERSION     DASVPL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206686"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2928
                     /locus_tag="VF517_04430"
     CDS             <1..2928
                     /locus_tag="VF517_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX8052216.1"
                     /translation="FLSYCAAGRAAAHHALLAELLELYDVRYAALKDARSALDFEDLE
                     LRARDLLRARPDLCTTVAERYRQVMVDEFQDTNKLQNEIVDLVAGERLFAVGDERQSI
                     YGFRHADVELFRERGAAAAAAGRAASLRTNFRSRPEVLAAVNAAFGSVWEEHEPLAPA
                     PGSSSTPPRMDPAVELLMVDRSKPRWEAAVGADAFGTGVGESIWRGAEARLLAKRIGE
                     LAGPGHAFEYGEVAILLRASTDMALYERALVERGIPAYSHGGRGFSEAQQVGDLRAYL
                     AALANPLDELALTMLMASPLVGASLGCVAAIARRSKRMRRDPWWALEQAFRPGGDGSD
                     GLAAGLPADDRARVAAFVERFARERAEAPRLALETVIDRAVTESGYDRVVLAMPGGDR
                     RLANVRKLMRIARRFEAAEGRDLRRFIDHLDEREELRAHEGEAPVEGEARTPAVRLMT
                     IHAAKGLEFPVVCVADLGRPMRGGDDGGIQVAEDGRVGLRLASLSGESRGALEWDRLE
                     EEQTARAEEEERRIFYVAMTRAEEHLVLSGATDLSKWPEPRPLREPLDWIWRAVAPGA
                     KDLLDGAPVGVDEYDTDHGHVRVACTALSPASVDELLASEDRRPGEPAAAAAEPPAAA
                     SPPAAADPPAAASPPAAAEPAELPASPPRDRLAAPAQPLPVARLSYSSLEGYRRCGYR
                     FYLERVARLGSLDNAALAGRSRAAEAAAANAAAAPEDGQLVLALDTARPIAAPTEGIT
                     PLLRGTIVHQLLERVDMRAPETPDADAIERSLRGHGSPAEPEEIEWIATLIEGFVRSP
                     LRERIAAAQRVRTELAFAFELPAADRPPVLVNGVVDVHAEEPDGLLIVDYKTDPLDGQ
                     DPAAVVERGYTTQRLVYALAGLRAGAERVEVAYSLLEAPNDPVAATFGPADRETLEHE
                     LTALAGGLLGGDFRPTDTPHRDLCWTCPGRPALCSWGPEMTLRERPANGSVRPPAYVR
                     RSL"
     gene            2940..>3509
                     /locus_tag="VF517_04435"
     CDS             2940..>3509
                     /locus_tag="VF517_04435"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX8052217.1"
                     /translation="MDAVTVSAYAVKIQVAAKRAGSATVEGEVQKPRKTGGGAFMFDL
                     TDGRSRLSCKVLPRRLRGGISHVPKNGDLVRIQAAHPDFWPQAGLLSVVVEDIELAGD
                     GELLRRREELLATLQREGLTDRAGFPALPRFPRAVGIVAGSDSDALKDVLQGLRERYP
                     IARAVTACCPVQGAAAPDAVVAALARLDLH"
BASE COUNT          427 a         1300 c         1350 g          432 t
ORIGIN      
        1 ttcctgagct actgcgcggc cggccgcgcc gccgcgcacc acgcgctgct ggccgagctg
       61 ctcgagctct acgacgtgcg ctacgccgcg ctgaaggacg cgcgctcggc actcgacttc
      121 gaggacctcg agctgcgcgc ccgcgacctg ctgcgcgcgc ggcccgacct ctgcacgacc
      181 gtggccgagc gctaccggca ggtgatggtg gacgagttcc aggacaccaa caagctccag
      241 aacgagatcg tcgacctggt cgcgggcgag cggctgttcg ccgtcggcga cgagcggcag
      301 tcgatctacg gcttccggca cgcggacgtg gagctgttcc gcgagcgcgg tgcggcggcg
      361 gccgcggcgg ggcgcgcggc gagcctgcgc acgaacttcc gcagccgtcc cgaggtgctg
      421 gcagcggtca acgcggcgtt cgggtcggtg tgggaggagc acgagccgct ggcgccggcg
      481 ccgggcagct cgtccacgcc gccgcggatg gacccggcgg tcgagctgct catggtcgat
      541 cgctccaagc cgcgctggga ggccgccgtg ggcgccgacg ccttcggcac cggcgtgggg
      601 gagagcatct ggcgcggcgc cgaggcgcgg ctgctcgcca agcggatcgg tgagctggcg
      661 gggccggggc acgcgttcga gtacggcgag gtggcgatcc tgctgcgggc ctcgaccgac
      721 atggccctgt acgagcgcgc actggtcgag cgggggattc ccgcgtactc gcacggcggg
      781 cgcgggttct ccgaggcgca gcaggtgggc gacctgcgcg cctacctggc ggcgctcgcc
      841 aacccgctcg acgagctggc gctgacgatg ctgatggcat cgccgctcgt gggggcgtcg
      901 ctcggctgcg tggcggcgat cgcgcggcgg tcgaagcgca tgaggcgcga cccgtggtgg
      961 gctttggagc aggcgttccg gccgggcggc gacggctccg acgggctcgc cgccggcctg
     1021 cccgcggacg accgcgcgcg ggtggcggca ttcgtggagc gcttcgcgcg cgagcgggcg
     1081 gaggcgccgc ggctggcgct cgagaccgtg atcgaccggg cggtgaccga gtccggctac
     1141 gaccgggtcg tgctcgcgat gccgggcggc gaccggcggc tcgcgaacgt gcgcaagctg
     1201 atgcggatag cgcggcggtt cgaggcggcg gaggggcgcg acctgcggcg gttcatcgac
     1261 cacctcgacg agcgcgagga gctgcgcgcg cacgagggcg aggcgccggt cgagggcgag
     1321 gcgcggacgc ccgccgtgcg gctgatgacg atccacgcgg cgaaggggct cgagttcccg
     1381 gtggtgtgcg tcgccgacct ggggcggccg atgcgcggcg gcgacgacgg tgggatccag
     1441 gtggccgagg acggccgggt cggactgcgg ctggcgtcgc tgtcgggcga gtcgcgcggg
     1501 gcgctcgagt gggaccggct cgaggaggag cagaccgcgc gcgccgagga ggaggagcgg
     1561 cggatcttct acgtggcgat gacccgcgcg gaggagcacc tggtgctgag cggagcgacc
     1621 gacctgtcga agtggcccga gccgcgaccg ctgcgcgagc ccctggattg gatctggcgc
     1681 gcggtggccc cgggggcgaa ggatctgctc gacggcgctc cagtaggtgt ggacgagtac
     1741 gacaccgacc acggccacgt ccgcgtggcc tgcacggctt tgtcgccggc gagcgtggac
     1801 gagctgctgg cgtcggagga tcgccggccg ggggagcccg ccgcggccgc cgcggagccg
     1861 cccgccgccg cttcgccccc cgccgccgcg gacccgcccg ccgccgcttc gccccccgcc
     1921 gccgccgagc ccgccgagct ccccgcgagc ccgccccgcg accggctcgc cgccccggcc
     1981 cagccgctcc ccgtcgcccg cctcagctac tcctccctcg agggctaccg ccgctgcggc
     2041 taccgcttct acctcgagcg ggtcgcgcgg ctcggctcgc tcgacaacgc cgccctcgcc
     2101 gggcgctcgc gcgccgccga ggccgcggcc gccaacgccg ccgccgcgcc cgaggacggg
     2161 cagctcgtgc tcgcgctcga caccgctcgc ccgatcgctg ccccaaccga ggggatcaca
     2221 cccctgctcc ggggaacgat cgtccaccag ctactcgagc gggtcgacat gcgcgccccg
     2281 gagacaccgg acgccgacgc gatcgagcgt tcgctgcgcg gccacggctc ccccgcggag
     2341 cccgaggaga tcgagtggat cgcgacgctc atagagggct tcgtccgctc gcccctgcgc
     2401 gagcggatcg cggccgcgca gcgcgttcgc acggagctcg cgttcgcctt cgagctcccg
     2461 gcggcggacc gcccacccgt gctcgtgaac ggcgtcgtgg acgtgcacgc cgaggagccg
     2521 gacggcctgc tgatcgtgga ctacaagacc gacccgctcg acggccagga cccggccgcg
     2581 gtggtcgagc gcggttacac gacccagcgc ctcgtctacg cgctggcggg cctgcgcgcg
     2641 ggagcggagc gggtcgaggt cgcctacagc ctcctcgagg cgccgaacga cccggtcgcg
     2701 gcgaccttcg gcccggccga ccgcgaaacg ctcgaacacg agctgaccgc gttggcgggc
     2761 ggcctcctcg gcggcgactt ccgccccacc gacacgccgc accgcgatct ctgctggacc
     2821 tgcccgggcc ggcccgccct ctgctcctgg ggcccggaga tgaccctgcg cgagcgcccg
     2881 gctaacggct ccgtccgccc cccggcttat gttcgtcggt ccctatgagc cggatcgcca
     2941 tggacgcagt caccgtctcc gcctacgcgg tgaagatcca ggtcgcagcc aagcgcgccg
     3001 gctcggccac ggtcgagggc gaggtgcaga agccgcgcaa gacgggcggc ggcgccttca
     3061 tgttcgacct caccgacggc cgcagcaggt tgagctgcaa ggtcctcccg cgccggctcc
     3121 gtggcggcat ctcgcacgtg ccgaagaacg gtgacctggt tcgcatccag gcggcgcatc
     3181 cggacttctg gccgcaggcg ggcctcctga gcgtcgtcgt cgaggacatc gagctcgcgg
     3241 gcgacggcga gctgctgcgc cggcgcgagg agctgctcgc caccctccag cgcgagggcc
     3301 tgaccgaccg cgccgggttc cccgcgctgc cgcgcttccc acgcgcggtg gggatcgtcg
     3361 cgggcagcga ctcggacgcg ctgaaggacg tcctccaggg cctgcgcgag cgctacccga
     3421 tcgcgcgggc ggtcaccgcc tgctgcccgg tgcagggagc ggccgcgccc gacgcggtgg
     3481 tcgcggcgct ggcgcggctg gacctgcac
//
LOCUS       DASVPL010000095         8363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_128535, whole genome shotgun sequence.
ACCESSION   DASVPL010000095 DASVPL010000000
VERSION     DASVPL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128535"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..2062)
                     /locus_tag="VF517_04440"
     CDS             complement(98..2062)
                     /locus_tag="VF517_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX8052218.1"
                     /translation="MLLPDRAGVLHPSSESEVQELVASARASNVPLRVRGTGHSVEPA
                     ILARHGIHVALDRLDTIDYDDARMRVTVGAGVRLGGNPRDGSAVETGLCWRLEQRGWA
                     LPVTAGITHQTVAGFLMTGSAGGSRRHALTEQVVGLRLIDGTGAIHDLHPGDERFDAA
                     GVSMGLLGVITAITLQCVERFDVEGVERIVSAADADPAALLRDNEYARAYWWPQRGVD
                     KLSTWTARSIPGPPSPDPRPYRLLPAVAGSTTPAQAVAGTAIGAACRAGRWNRGLAAL
                     YNAFLPEGEEQHFRDAWWRVLPQDDELDERFFDSTWCELWIPADATDEAIRRLREHYR
                     TGGHPATGAYTLEIYGAPPSRFWLSPGYARESTRLNICWHESTKGDPRETYFPQFWSL
                     LGDLDFRMHWAKHRFPDPPRAARHARAAHPRLGEFLELRENMDPDGVFLSDYWREQLG
                     VSDAPRRPAPQDQNGRAPRIAGTPGRRWPLVFKLRPSDASFGERAPHAIDVTETLAAS
                     PEAIYDGIVNLTEARDWLPDFVRARWIAGPNERGEQVVEETFKFMSQRVRTFVAEPGR
                     HWMASIDACTLPLGRELMEDLELEPLPGGGTRVRWRYYYDPYTALKPIRKLLHKEFDH
                     MLRLSLEQLDAHITGEPVTDDGAVVHTPAG"
     gene            complement(2062..3618)
                     /locus_tag="VF517_04445"
     CDS             complement(2062..3618)
                     /locus_tag="VF517_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8052219.1"
                     /translation="MIRSDQVPAEADVVVVGAGLSGLTAARELEAAGASVVVLEARER
                     VGGRLLGVRVGEGAVVDLGGEYFGPLGHKIIEAARSVGVPEQKVNNTGDKLLELSGRV
                     RRYKGYIPNAGALVLADSAQGLLRFERMVRQVPPAAPWTAAKAREWDSQTLATWVRRN
                     FATRGARELFEMATEAIWCASTADFSLLHALFYSRSYESFEYLGSVRKGSQERRFQGG
                     AVAIPERMAEGLDAPIALGCAVRDVAHDRGRVRVSGEGFAVSARRAIVALPPVMAGRI
                     VYDPPLPGQRDQLVQRLNPGSALKYVAVYDRAFWRENGLCGQATSTRGPLAAIFDTIP
                     AGGNCAVIGGFGGGRTARELALLPERARREAVLDQFVRLFGERARTPDEFHEKNWSDD
                     PWARGCYNAIATTGALTAFGPALRKPVGRIHWAGAETGIHANGSMGGAVDAGERAAAE
                     VLEELAMERGGEGASGAGAAASSAGAAAGAGEAAGSTPDAHAAAAPSAGNGASAPDGA
                     SAPAGAPAAT"
     gene            complement(3635..4447)
                     /locus_tag="VF517_04450"
     CDS             complement(3635..4447)
                     /locus_tag="VF517_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8052220.1"
                     /translation="MPDYETLIYEQDEHVVTLTYNRPDQHNAVSRTMNEELHHAWQRF
                     RDDETAFVLVITGAGETTFCAGWDLQDASELTEMPDFDEFRTQLYNSPGDCGYTRRAD
                     IFKPVIAAVNGYAFAAGLETALLADIRIAAENAEFGALERRWNIVGGDGLTVRLPLVV
                     GYAKAMELIITGRRIDVHEAERIGLVNEIVPRGESLARAQALAHEIAALPQGAIRSDK
                     ESVMRGVGHTLEERLRIEFEMALSMFMRRDKHTVGASAFKRGERPEWPHHGL"
     gene            4468..5052
                     /locus_tag="VF517_04455"
     CDS             4468..5052
                     /locus_tag="VF517_04455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8052221.1"
                     /translation="MPDLHVDLGTGDGKYVLRCARANPERHHVGVDALEAPMAASIRR
                     AAAKPARGGVENARFIVRDALDPPADLLGRASLVTVNYPWGSLLRAVAAPEPAGLAAV
                     VSLLRPGGRLVALLNASASEDSSYAERLELPALDGEHIEERLVPGWSAEGLRDVSARL
                     LDAGEEPPHRTTWGQRLVRGSGRETLLVSGTLQS"
     gene            complement(5043..5999)
                     /locus_tag="VF517_04460"
     CDS             complement(5043..5999)
                     /locus_tag="VF517_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HEX8052222.1"
                     /translation="MGAITGTAAGHPNIRATHGKTLELTREAEIGARATCVVGVATRL
                     DEEALAELHGRVELTLAAGGETVHVRGRLNPAFRPGDPLVVRRAPDVARNAVVVGADR
                     AAADVARSFVAAAASEARIAVRVEELAHETAPGVLVVAPAPAPASGAPAEDLGQVRGH
                     IRPKDGPVLRTDPNSVTRALQEGQRVTLCVDLATDAEARTAIAQAHAAGHTVLPAPTL
                     SLNDAVLAVAGIANAELQMDGAELRPREAELDARTALIANAPADRVPKWLRKATRSGH
                     TRGVIGLDLGTEREQFVSWRAGEAVEVPGARGRTATLVLVQD"
     gene            complement(6068..6562)
                     /locus_tag="VF517_04465"
     CDS             complement(6068..6562)
                     /locus_tag="VF517_04465"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8052223.1"
                     /translation="MRRYLLVAAVLVGGLIVLELATHDGGDDGPGAPPLPAAVLVPPK
                     VTVESLRGKPAVVNFWASWCEPCRKEAPLLQRFYERNRGRIGVVGVSYTDNAPGARKF
                     IRRYGWTFPNLSDPDGLAGSEYRLLGLPVTVILDSDGRIATQLRGPQTEAKLAAAVRA
                     AEES"
     gene            complement(6568..7221)
                     /locus_tag="VF517_04470"
     CDS             complement(6568..7221)
                     /locus_tag="VF517_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX8052224.1"
                     /translation="MPSRFRLPLGLVLGVALILGATACGDDEGGGGSKQNSVAGDQKK
                     GSMPDYAGAVATPPKPAPPLVLEDSTGERFDISQHRGKAVLVTWLYVNCPDVCPLIVS
                     NLKVAQTKLGAKAKDMVIVAVSTDPRRDTPKAVNKFIAGRGMSGRMQYLVGGEGELGR
                     TWKNWGIVAEPAAANPELIEHSSPIYGISGSGKITTLYPANFKPEQIVRDVPLLAQQ"
     gene            complement(7282..>8363)
                     /locus_tag="VF517_04475"
     CDS             complement(7282..>8363)
                     /locus_tag="VF517_04475"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX8052225.1"
                     /translation="ALGDHVAAGFPRRRRVLALGDHVAAGFPRRGRRVPSLARPPRVA
                     SPDAAPRLPALAVPRRRMLALAGAAASLLVVIGVALSVSGTDTRDAAPVGETAEPAAQ
                     APSDDLALRTPPAGGAVQVPPAGAGAARVAPGERERRIERSAELTLAAPEDELDRVAD
                     SIVGVTDRHEGFVLRSSVSTGDDSEGGGTFDLRIPAASLQEALRDLSKLGHVRARSQS
                     GRDVTPAFVSSRDRLSAARAERRSLLRRLAAADSDRAAEAIRRRLDAVSLRIDRLRRE
                     LGALRERTDYVAVAVTLEAEGDDGGAATGGTREALDDSLGLLAGSLNLALRALGVLIP
                     VGIVAGLGWLGGTMLRRRRREAALR"
BASE COUNT         1054 a         3038 c         3142 g         1129 t
ORIGIN      
        1 taggagggac gctcgatgtg cgcgagcgcg agcgccggca gcgccagcgc cacgatcgca
       61 gcggcgatgg ctgcccacgc catgcgagtg ccccgggtca tccggcagga gtatggacga
      121 cggcgccgtc gtctgtcacc ggttctccgg tgatgtgcgc gtcgagttgc tcgaggctga
      181 gccgcagcat gtggtcgaac tccttgtgca ggagcttgcg gatcggcttc agcgcggtgt
      241 acgggtcgta gtagtagcgc cagcgcacgc gcgtgccgcc gccgggcagg ggctcgagct
      301 cgaggtcctc catcagctcg cggccgagcg gcagcgtgca cgcgtcgatc gacgccatcc
      361 agtgcctgcc gggctcggcg acgaaggtgc gcacgcgctg gctcatgaac ttgaaggtct
      421 cctcgaccac ctgctcgccg cgctcgttcg gcccagcgat ccagcgcgcc cggacgaagt
      481 cgggcagcca gtcgcgggcc tcggtgaggt tcacgatgcc gtcgtagatc gcctccggcg
      541 aggcggcgag cgtctccgtc acgtcgatcg cgtgcggtgc gcgctcgccg aagctcgcat
      601 cgctcgggcg gagcttgaag acgagcggcc agcggcggcc gggggtgccg gcgatgcgcg
      661 gcgcgcggcc gttctggtcc tgcggggccg ggcggcgggg cgcgtcgctc acgccgagct
      721 gctcgcgcca gtagtcggac aggaacacgc catcggggtc catgttctca cgcagctcca
      781 ggaactcgcc gagtcgtggg tgggcggcgc gggcgtggcg ggcggcgcgg ggcgggtccg
      841 ggaaccggtg cttggcccag tgcatgcgaa agtcgaggtc gccgagcagg ctccagaact
      901 gcgggaagta ggtctcgcgc gggtcgccct tcgtgctctc gtgccagcag atgttgaggc
      961 gcgtggactc gcgggcgtag ccgggggaga gccagaagcg gctcggcggg gcaccgtaga
     1021 tctcgagcgt gtaggcgccg gtggccgggt gcccgcccgt gcggtagtgc tcgcgcaggc
     1081 ggcggatcgc ctcgtccgtg gcgtcggccg ggatccacag ctcgcaccag gtgctgtcga
     1141 agaaccgctc gtcgagctcg tcgtcctggg ggagcacgcg ccaccaggcg tcgcggaagt
     1201 gctgctcctc gccctccggg aggaacgcgt tgtagagcgc ggcgaggccg cggttccagc
     1261 gcccggcgcg gcaggcggcg ccgatcgcgg tgccggcgac ggcctgcgcg ggcgtggtcg
     1321 acccggcgac ggcggggagg agacggtacg ggcgcgggtc cggcgacggc ggcccgggga
     1381 tcgagcgggc ggtccaggtc gagagcttgt ccaccccgcg ctggggccac cagtacgcgc
     1441 gggcgtactc gttgtcgcgc aggagcgcgg ccgggtctgc gtccgccgcg gagacgatgc
     1501 gctccacgcc ctccacgtcg aagcgctcga cgcactggag cgtgatcgcc gtgatcacgc
     1561 ccagcaggcc catcgacacg cccgcggcgt cgaagcgctc gtcgccgggg tggaggtcgt
     1621 ggatcgcgcc cgtgccgtct atcagtctca ggccgacgac ctgctcggtg agcgcgtggc
     1681 ggcggctgcc tccggccgag ccggtcatca ggaagccggc gacggtctgg tgcgtgatgc
     1741 ccgcggtcac cggcagcgcc cagccgcgct gctcgagccg ccagcagagc ccggtctcga
     1801 ccgcgctccc gtcgcgcggg ttgccgccga gccgcacgcc cgcgcccacg gtcacccgca
     1861 tgcgcgcgtc gtcgtagtcg attgtgtcga ggcggtcgag cgcgacgtgg atgccgtggc
     1921 gggcgaggat cgcgggctcg accgagtggc ccgtgccgcg gacgcgcagc ggcacgttcg
     1981 acgcgcgggc cgaagcgacg agttcctgca cctcggactc gctcgacggg tggaggaccc
     2041 cggcgcggtc ggggaggagc actacgtggc ggcgggcgcg cccgccgggg cggaggcgcc
     2101 gtccggagcg gaggcgccgt tgccggcgga gggggccgcg gccgcgtggg cgtcgggcgt
     2161 cgagccggcg gcttcgcccg cgcccgcggc ggcgcccgcg ctcgaagcgg cggcgcccgc
     2221 gcccgaagcg ccttcgcccc cgcgctccat cgccagctcc tcgagcacct ccgccgccgc
     2281 tcgctcgccc gcgtccacgg ctccgcccat cgagccgttc gcgtggatgc ccgtctccgc
     2341 gcccgcccag tggatccggc cgaccggctt gcgcagggcg gggccgaagg cggtcagcgc
     2401 acccgtggtc gcgatcgcgt tgtagcaacc gcgcgcccac ggatcgtcgg accagttctt
     2461 ctcgtggaac tcgtccggag tccgcgcgcg ctcgccgaac agccgcacga actggtcgag
     2521 caccgcctcg cgccgcgcgc gctcgggcag cagcgccagc tcgcgcgccg tgcgcccgcc
     2581 gccgaagccg ccgatcaccg cgcagttgcc gccggcgggg atcgtgtcga agatcgcggc
     2641 gagcgggccg cgggtgctcg tggcctgccc gcacagcccg ttctcgcgcc agaaggcgcg
     2701 gtcgtagacc gccacgtact taagcgccga ccccgggttg agccgctgga cgagctggtc
     2761 gcgctggccc ggcagcggcg ggtcgtacac gatgcgtccc gccatgaccg gcggcagcgc
     2821 cacgatcgcc cggcgggcgc tcaccgcgaa gccctcaccg gacacgcgca cgcgcccgcg
     2881 gtcgtgcgct acgtcgcgca ccgcgcagcc gagcgctatc ggcgcgtcga ggccctcggc
     2941 catccgctcg gggatcgcca cggcgccgcc ctggaagcgg cgctcctggc tccccttccg
     3001 cacgctcccc aggtactcga acgactcgta gctgcgcgag tagaagagcg cgtgcaggag
     3061 cgagaagtcg gcggtcgacg cgcaccagat cgcctcggtg gccatctcga acagctcgcg
     3121 cgcgccgcgg gtggcgaagt tgcgccgcac ccaggtcgcc agcgtctgcg agtcccactc
     3181 gcgcgccttc gcggccgtcc acggcgccgc cggcggcacc tgccgcacca tccgctcgaa
     3241 gcgcagcagc ccctgcgccg agtcggccag cacgagcgcg cccgcgttcg ggatgtagcc
     3301 cttgtagcgg cgcacgcggc cgctcagctc gagcagcttg tcgccggtgt tgttgacctt
     3361 ctgctccggc acccccaccg accgcgccgc ctcgatgatc ttgtggccga gcggaccgaa
     3421 gtactcgccg ccgagatcga ccacggcgcc ctcgccgacg cgcaccccga gcagccgccc
     3481 gcccacgcgc tcgcgcgcct cgagcaccac gaccgacgcg cccgcggcct ccagctcgcg
     3541 cgccgcggtg agccccgaga ggcccgcgcc cacgacgacg acgtcggcct cggctggcac
     3601 ctggtcggag cggatcacgg cgccggggat cctctcacag cccgtggtga ggccactcgg
     3661 gccgctcgcc gcgcttgaaa gcgctcgccc cgaccgtgtg cttgtcacgc cgcatgaaca
     3721 tcgacagcgc catctcgaac tcgatccgca gccgttcctc gagcgtgtgg ccgacgccgc
     3781 gcatcacgga ttccttgtcg ctccgtatgg cgccctgcgg gagcgcggcg atctcgtgcg
     3841 cgagcgcctg cgcccgcgcg agcgactcgc cgcgcgggac gatctcgttc accagcccga
     3901 tccgctccgc ctcgtgcacg tcgatccgcc gaccggtgat gatcagctcc atcgccttcg
     3961 cgtagccgac cacgagcggc aggcgcacgg tcaggccgtc gccgccgacg atgttccagc
     4021 gtcgctcgag cgccccgaac tccgcgttct cggcggcgat gcggatgtcg gcgagaaggg
     4081 cggtctcgag gccggcggcg aacgcgtagc cgttcacggc ggcgatcacc ggcttgaaga
     4141 tgtccgcgcg gcgcgtgtag ccgcagtcgc cgggcgagtt gtagagctga gtgcggaact
     4201 cgtcgaagtc cggcatctcg gtgagctccg aggcgtcctg taggtcccac cccgcgcaga
     4261 aggtcgtctc gccggcgccg gtgatcacga ggacgaatgc ggtctcgtcg tcgcggaagc
     4321 gctgccacgc gtggtggagc tcctcgttca tcgtgcggct gaccgcgttg tgctggtcgg
     4381 gccggttgta ggtgagcgtg acgacgtgct cgtcctgctc gtagatcagc gtctcgtagt
     4441 cgggcatggc gcgtaccgta cgcctcggtg cccgacctgc atgtagacct cgggacgggt
     4501 gacgggaagt acgtgctgcg ctgcgcgcgg gcgaatcccg aacggcatca cgtaggagtc
     4561 gatgcgctgg aggcgccgat ggcggcgtcg atccggcgtg cggcggccaa gcctgctcgg
     4621 ggcggagtgg agaacgctcg gttcatcgtg cgcgatgcgt tggatccgcc cgcggacctg
     4681 ctcggtcgcg cttcgctcgt gacggtgaac tacccgtggg ggtcgctgct gcgggcggtg
     4741 gccgcgccgg agccggccgg gctggcggcg gtcgtgtcct tgttgcggcc cggcgggcgg
     4801 ctcgtcgcgt tgctcaacgc gagcgcttcg gaggacagct cgtatgccga gcggttggag
     4861 ttgccggcgc tcgacggcga gcacatagag gagaggctcg ttcccggttg gtctgccgag
     4921 ggtctccgtg atgtctcggc gcggctgctc gatgcggggg aggagccgcc gcaccggacg
     4981 acgtggggtc agcggctagt gcggggctcc ggtcgcgaga cgctgctcgt gtccggcacg
     5041 cttcagtcct gaacgaggac gagggtggcg gtgcgcccgc gcgctcccgg gacctcgacg
     5101 gcttcgccgg cgcgccatga cacgaactgc tcgcgctcgg tgccgaggtc gaggccgatc
     5161 acgccgcggg tgtggccgga gcgcgtggcc ttgcgcagcc acttcgggac gcggtcggcc
     5221 ggggcgtttg cgatgagagc cgtgcgcgcg tcgagctcgg cctcgcgggg gcggagctcg
     5281 gcgccgtcca tttggagttc ggcgttcgct attcccgcca ccgctagaac ggcgtcattt
     5341 agggagaggg tcggggcggg gaggacggta tggcccgccg cgtgggcctg cgcgatcgcg
     5401 gtcctcgcct ccgcgtccgt ggccaggtcg acacaaagcg tgacgcgctg accctcttgt
     5461 aacgctcgtg taacggagtt cgggtcggtt cggagaactg ggccatcttt tggtcgtata
     5521 tgacccctaa cttggcccag gtcctccgcg ggggcgccgg aggcgggggc gggggccggc
     5581 gcgaccacga ggacacccgg cgcggtctcg tgggccaact cctccaccct cactgcaatc
     5641 cgcgcttcgg acgcggcggc agccacgaag gagcgcgcga cgtcggcggc cgcgcggtcc
     5701 gcgcccacca cgacggcgtt acgcgccaca tcaggcgctc gccgcaccac aagcggatcg
     5761 cccggccgga acgccgggtt gagccggccg cgcacgtgca cggtctcccc gccggcggcg
     5821 agggtcagct ccacgcgccc gtgcagctcc gccagcgcct cctcgtcgag ccgagtcgcc
     5881 acgccgacca cgcacgtcgc ccgcgccccg atctcggcct cgcgagtcag ctccagcgtc
     5941 ttgccgtgcg tcgcgcggat gttcggatgg ccggcggctg taccggtgat agcgcccatc
     6001 gcggcaggct acgccgcgcg cacatcgatc gcgggcttcg cgccgggccg ccgccgcgcc
     6061 ccgaccctca gctctcctcc gccgcgcgca cggccgcggc gagcttcgcc tccgtctgcg
     6121 ggccgcggag ctgcgtcgca attcgcccgt cggagtcgag gatcaccgtc accggcaggc
     6181 cgagcaggcg gtactcgctc ccggccaggc cgtccgggtc gctcaggttc gggaacgtcc
     6241 acccgtagcg gcggatgaac ttgcgcgcac cgggcgcgtt gtcggtgtag ctcacgccca
     6301 ccacgccgat ccgcccgcgg ttgcgctcgt agaagcgctg gaggagcgga gcctccttgc
     6361 ggcacggctc gcaccagctc gcccagaagt tcacgacggc cggcttgccg cgcagcgact
     6421 cgacggtcac cttcggcggc acgaggaccg cggccgggag gggcggagcg cccggcccgt
     6481 cgtcgccccc gtcgtgcgtg gccagctcga gcacgatcag tccgcccacg aggacggcgg
     6541 ccacgagcag gtagcgccgc acggccccta ctgctgggcg aggagcggca cgtcgcggac
     6601 gatctgctcc ggcttgaagt tcgccgggta gagcgtggtg atcttccccg agccgctgat
     6661 cccgtagatg ggcgacgagt gctcgatcag ctccgggttg gcggcggccg gctcggcgac
     6721 gatgccccag ttcttccagg tccgtccgag ctcaccctcg ccgccgacca ggtactgcat
     6781 ccgcccgctc atgccgcgcc cggcgatgaa cttgttgacg gccttgggcg tgtcgcgcct
     6841 gggatcggtc gagaccgcca cgatcaccat gtccttcgcc ttcgcgccga gcttcgtctg
     6901 cgcgaccttg aggttgctca cgatcagcgg gcacacgtcg gggcagttca cgtacagcca
     6961 cgtgacgagc acggccttcc cgcggtgctg cgagatgtcg aaccgctcgc cggtcgagtc
     7021 ctcgagcacg agcggcggcg ccggcttcgg cggcgtggcc accgcgccgg cgtagtcggg
     7081 catcgagccc ttcttctgat cgccggcgac cgagttctgc ttgctcccgc cgccgccttc
     7141 atcgtcgccg cacgcggtgg cgcccaggat cagggcgacg ccgaggacga ggccgagcgg
     7201 caggcggaat cgcgaaggca cggcgggcag cctaggggac cgcgctaaac gcgtcgtgaa
     7261 gcccgccggg aagggccgcg gctaccgcag ggcggcctcg cgccggcggc ggcggagcat
     7321 ggtgcctccg agccacccga gccccgcgac gatccccacc ggaatcagca caccgagcgc
     7381 acgaagtgcg aggttcaggg agccggcgag gagaccgagc gagtcgtcga gcgcctcacg
     7441 cgtgccgccc gtcgcggccc cgccgtcgtc gccctcggcc tcgagggtga ccgcgaccgc
     7501 gacgtagtcg gtccgctcac gcagcgcgcc gagctcgcgg cgcagccggt cgatgcgcag
     7561 cgacaccgcg tcgaggcggc gccggatcgc ctcggccgcg cggtcgctgt cggcggctgc
     7621 gagccggcgc agcaggctgc ggcgctcggc ccgcgcggcg ctgaggcggt cgcgcgagga
     7681 gacgaacgcg ggcgtcacgt cgcgtcccga ctggctgcgc gcgcgcacgt gaccgagctt
     7741 cgagaggtcg cgcaacgcct cctggagcga cgcggcggga atgcgcaggt cgaacgtgcc
     7801 gccgccctcg gagtcgtcgc cggtcgagac cgacgagcgc agcacgaacc cctcgtggcg
     7861 gtcggtcacg ccgacgatcg agtccgccac gcggtcgagc tcgtcctccg gcgccgcgag
     7921 cgtgagctcg gcggagcgct cgatgcgccg ctcgcgctcg ccgggtgcga cgcgcgcggc
     7981 gccggcgccc gccggcggca cctgcaccgc gccgcccgcc ggcggagtcc gcagcgcgag
     8041 gtcgtcggag ggagcctgcg ccgccggttc ggcggtctcg cccacgggag cggcatcgcg
     8101 ggtatcggta ccggagacgg acagggccac gccgatcacg acgagcaggg aggccgcggc
     8161 gcccgcgagc gcgagcatcc gccggcgggg gacggccagc gcgggcaggc ggggcgccgc
     8221 gtccggggag gcgacgcgcg gcggccgggc cagcgagggg acgcggcgcc cccggcgcgg
     8281 gaacccggcc gcgacgtggt cgcccagcgc gagcacacgc cgccggcgcg ggaacccggc
     8341 cgcgacgtgg tcgcccagcg cga
//
LOCUS       DASVPL010000096         5173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_12345, whole genome shotgun sequence.
ACCESSION   DASVPL010000096 DASVPL010000000
VERSION     DASVPL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12345"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VF517_04480"
     CDS             <1..212
                     /locus_tag="VF517_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052226.1"
                     /translation="IARTSPKGGGATIQLDRGRRRVVKFRSGSSRNRVLVATVGTKGA
                     GRHVLRLVTLGGGPVEIDGVGVAAR"
     gene            complement(209..1303)
                     /locus_tag="VF517_04485"
     CDS             complement(209..1303)
                     /locus_tag="VF517_04485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8052227.1"
                     /translation="MPRLVGLSQGDPYSARTNSGVAKFLYDALERRYGLAGRYSVELT
                     QPQRALAAALTFHPSRPRWRGRYYWQWALANRLRSRNAAARLREVEGGVDLAVQIFSL
                     FQARGAPYVLYTDNTMAITLEHNPGWAPDDPQSALDWERKVFNEARHVFVVGQIMADS
                     MVEHYGVPEERISIVSGGSNFHPLPEPKQRTHEPVILFVGRFWEHKGGPHLIEAFRAV
                     RARLPEARLVVLGTDDAPTEPGVEVLGTIDDRARVAELYADAGVFCLPSRWEGFGGSL
                     AEAMGHSLPCVTTAVGALPEVVLDGETGLVVPREDPEALGAALLRILEDPKWGDELGR
                     AGRRRVEEYLTWDATVERMGPGLEKALAGG"
     gene            complement(1308..1604)
                     /locus_tag="VF517_04490"
     CDS             complement(1308..1604)
                     /locus_tag="VF517_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX8052228.1"
                     /translation="MESGGATIELPRVGDPGSGLGGAWRVIVLNDNHNTFDGVAQALA
                     RIVPGVTLEKGHAIANQIHNTGQAIVWSGQREPAEHYWEQLREAGLTMAPLEQG"
     gene            complement(1604..2428)
                     /locus_tag="VF517_04495"
     CDS             complement(1604..2428)
                     /locus_tag="VF517_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8052229.1"
                     /translation="MARPIDVMHMGRDRVICAYEQDGVIVDPGPATSIGALDGIEPRA
                     ILLTHIHLDHAGGTGTLVGRHPDVKVYVHEVGAPHVVDPTRLLRSAERLYGDWMDRLW
                     GEVVPVPEENVVVLHGGEVVEGFRVAYTPGHAGHHVAYLNESTGEAFTGDVAGVRIPP
                     FEHTIAPTPPPEIDVELWLRSIDLVESWEPERLCLTHFGPVDEPAAQLARVREALRLQ
                     LEWADQGADAFAYEYGMTVSKALPEEEAVRLQQAAPAEQLYLGLERYLTKRAEATA"
     gene            2320..4440
                     /locus_tag="VF517_04500"
     CDS             2320..4440
                     /locus_tag="VF517_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HEX8052230.1"
                     /translation="MLRCSSPAPDPRSRRPARTRRSRGRAPCASRQSAAPPGPPYNST
                     MEPRLPEPDRPWVMRTYAGHSDARESNALYRKNIEKGQTGLSIAFDLPTQTGYDPDHE
                     LARGEVGKVGVSIAHKGDMHTLLDGIPLEEMNTSMTINATAAWLLALYVAAAEENGVD
                     AKVLQGTTQNDIIKEFLSRGTYAFPPGPSMRLIADMIAHCVDEVPKWNPINVCSYHLQ
                     EAGATPVQEIAYALSTAQAVLDQVRERVPEETFPRVFGRISFFVNAGIRFVEEHAKLR
                     AMGQLWREMGRERYGVEDERYLRFRYGVQVNSLGLTEAQPENNVIRIVLEALAVTLGR
                     DARARALQLPAWNEALGLPRPWDQQWSLRIQQILALETDLLEYPDIFEGSKVMDGLVA
                     ELVEGSRQEMAVVAEHGGAVEAVDYMKTQLVESHRARVGRIESGDLKVVGQNTFTSTE
                     PSPLAEGEDGGILTVDPNVERDRIAAVEEWRSQRDEDAVRESLEQLRAAAEDEGTNIM
                     PATLACARAGVTTGEWGGTLREVFGEYRAPTGVEHAAGAIEDDRLTELREKVEAVSET
                     LGRRVKILVGKPGLDGHSNGAEQIAVRARDAGMDVVYEGIRLTPARIAATAVQEGVHV
                     VGLSILSGAHRELIPDVLRELSDAGAAVPVVVGGIIPPADEKALLEAGVARVYTPKDF
                     EITRIMGDIVELVAEHHGAAAAAA"
     gene            4437..>5173
                     /locus_tag="VF517_04505"
     CDS             4437..>5173
                     /locus_tag="VF517_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8052231.1"
                     /translation="MTDPADLGQRLRDGDRSAAPAALNVIEDRSPAGRERARILLAEV
                     APARLGGEAGAHLIGVTGPPGAGKSSLLSALVAHWREADRSVAVLAVDPSSKRSGGSL
                     LGDRARIEHDPRDDGVLIRSTAAGTRLGGLAAPTREAAQVLAPAYDLVVVETVGVGQS
                     ETDVEELCDTTAVVVQPASGDTLQFIKAGIMEVPDVLVVTKADLGDSARRARRDLIAA
                     LRALGSSGTPVVSVSSTPPPRGIEELAA"
BASE COUNT          719 a         1909 c         1861 g          684 t
ORIGIN      
        1 tgatcgcgcg cacgagcccg aagggcggtg gcgcgacgat ccagctcgac cgcgggcgcc
       61 gcagggtcgt caagttccgc tcgggctcgt cccgcaaccg cgtcctggtg gccacggtcg
      121 gcacgaaggg cgcgggccgc cacgtgctgc ggctggtcac gctgggcggc ggcccggtcg
      181 agatcgacgg cgtgggcgtg gcggcacgct agccgccggc cagcgccttc tcgagcccgg
      241 gccccatccg ctccaccgtc gcgtcccagg tcaggtactc ctcgacccgg cggcgcccgg
      301 cgcggcccag ctcgtcgccc cacttggggt cctcgaggat gcgcagcagc gcggcgccga
      361 gcgcctcggg atcctcgcgc ggcaccacca ggccggtctc gccgtcgagc acgacctcgg
      421 gcagcgcgcc caccgcggtc gtcacgcacg gcagcgagtg ccccatcgcc tccgcgagcg
      481 acccgccgaa gccctcccag cgcgacggca ggcagaacac gcccgcgtcg gcgtagagct
      541 cggccacccg cgcgcggtcg tcgatcgtgc ccagcacctc cacgccgggc tcggtcggcg
      601 cgtcgtcggt ccccagcacg accagccgcg cctcgggcag gcgcgcgcgc accgcgcgga
      661 acgcctcgat cagatgcggc ccgcccttgt gctcccagaa gcgccccacg aacaggatca
      721 cgggctcgtg cgtgcgctgc ttgggctcgg gcagcggatg gaagttggac ccgccgctca
      781 cgatgctgat ccgctcctcc ggcacgccgt agtgctcgac catcgaatcg gccatgatct
      841 gcccgaccac gaacacgtgg cgcgcctcgt tgaagacctt gcgctcccag tcgagcgccg
      901 actgcgggtc gtccggggcc cagccgggat tgtgctcgag cgtgatcgcc atggtgttgt
      961 cggtgtagag cacgtagggc gcgccgcgtg cctggaacag cgagaagatc tgcaccgcga
     1021 ggtccacccc gccctcgacc tcgcgcagcc gcgccgccgc gttgcgcgag cgcagccggt
     1081 tggccagcgc ccactgccag tagtagcgcc cgcgccagcg cggccgcgag gggtggaacg
     1141 tgagcgcggc ggccagcgcc cgctgcggct gggtcagctc gacgctgtag cggccggcca
     1201 gcccgtagcg gcgctcgagc gcgtcgtaga ggaacttcgc gacgcccgag tttgtccggg
     1261 ccgagtacgg gtcgccctgt gagagcccga cgagccgcgg cacgcggcta gccctgctcg
     1321 agcggcgcca tcgtgagccc cgcctccctg agctgctccc agtagtgctc ggccggctcg
     1381 cgctggccgg accagacgat cgcctggccg gtgttgtgga tctggttggc gatcgcgtgg
     1441 cccttctcga gggtcacgcc cgggacgatc cgggcaagcg cctgggccac gccgtcgaag
     1501 gtgttgtggt tgtcgttcag cacgatcacg cgccacgccc cgccgaggcc ggagcccggg
     1561 tcgcccacgc gcgggagctc gatggtggcg ccgcccgact ccactacgcg gtcgcctccg
     1621 ccctcttggt caggtagcgc tcgagcccca ggtagagctg ctcggccggc gcggcctgct
     1681 ggagccgcac ggcctcctcc tcgggcagcg ccttgctcac cgtcattccg tactcgtagg
     1741 cgaacgcgtc ggcgccctgg tcggcccact cgagctggag ccgcagcgcc tcgcgcacgc
     1801 gcgcgagctg ggcggccggc tcgtccaccg ggccgaagtg ggtcaggcag agccgctccg
     1861 gctcccacga ctcgacgagg tcgatcgagc gcagccacag ctcgacgtcg atctccggcg
     1921 gcggggtggg cgcgatcgtg tgctcgaacg gcgggatccg caccccggcc acgtcgccgg
     1981 tgaacgcctc gccggtcgac tcgttcaggt aggcgacgtg gtggcccgcg tgcccgggcg
     2041 tgtatgccac ccggaagccc tcgaccacct ctcccccgtg cagcaccacc acgttctcct
     2101 ccggcaccgg cacgacctcg ccccagagcc ggtccatcca atcgccgtag agccgctccg
     2161 cacttcgcag caggcgcgtc gggtcgacca cgtgcggcgc ccccacctcg tgcacgtaca
     2221 ccttcacgtc cggatgccgg ccgacgagcg tgccggtccc gcccgcgtgg tcgaggtgga
     2281 tgtgcgtgag gaggatcgcg cgcggctcga tgccgtcgag tgctccgatg ctcgtcgccg
     2341 gccccggatc cacgatcacg ccgtcctgct cgtacgcgca gatcacgcgg tcgcgcccca
     2401 tgtgcatcac gtcaatcggc cgcgccaccc gggccaccat acaattcgac catggagcca
     2461 cgactgcctg agccggatcg cccctgggtg atgaggacct acgcgggcca ctcggacgcc
     2521 cgcgagtcca acgcgctgta ccgcaagaac atcgagaagg gccagaccgg cctgtcgatc
     2581 gcgttcgacc tcccgaccca gacgggctac gaccccgacc acgagctcgc gcgcggcgag
     2641 gtcggcaagg tcggcgtctc gatcgcgcac aagggcgaca tgcacacgct cctcgacggc
     2701 atcccgctcg aagagatgaa cacgtcgatg acgatcaacg ccaccgcggc gtggctgctg
     2761 gcgctctacg tcgcggcggc cgaggagaac ggcgtcgacg ccaaggtcct ccagggcacg
     2821 acccagaacg acatcatcaa ggagttcctc tcgcgcggga cctacgcgtt cccgcccggg
     2881 ccgtcgatgc ggctgatcgc ggacatgatc gcccactgcg tcgacgaggt cccgaagtgg
     2941 aacccgatca acgtctgctc gtaccacctc caggaggcgg gcgcgacgcc cgtccaggag
     3001 atcgcctacg cgctctccac cgcccaggcg gtcctcgacc aggtgcgcga gcgcgtgccg
     3061 gaggagacct tcccgcgcgt gttcggccgg atctcgttct tcgtcaacgc cggcatccgc
     3121 ttcgtcgagg agcacgcgaa gctgcgcgcg atggggcagc tctggcgtga gatgggccgc
     3181 gagcgctacg gggtcgagga cgagcgctac ctgcgcttcc gctacggcgt gcaggtcaac
     3241 tcgctcgggc tcaccgaggc gcagccggag aacaacgtga tccggatcgt gctcgaggcg
     3301 ctggccgtca cgctcggccg cgacgcgcgc gcacgcgcgc tccagctccc cgcctggaac
     3361 gaggcgctcg ggctgccgcg tccctgggac cagcagtggt cgctgcggat ccagcagatc
     3421 ctcgcgctcg agaccgacct gctcgagtac cccgacatct tcgaggggtc gaaggtcatg
     3481 gacggtctgg tggccgagct ggtcgagggc tcgcggcagg agatggccgt ggtggccgag
     3541 catggcggcg ccgtcgaggc ggtggactac atgaagaccc agctcgtcga atcgcaccgt
     3601 gcgcgcgtcg ggcggatcga gagcggcgac ctcaaggtgg tcgggcagaa cacgttcacg
     3661 agcaccgagc cctccccgct cgccgagggc gaggacggcg ggatcctgac ggtcgaccct
     3721 aacgtcgagc gcgaccggat cgccgcggtc gaggagtggc gctcgcagcg cgacgaggat
     3781 gccgtgcgcg agtcgctcga gcagctccgc gccgccgccg aggacgaggg cacgaacatc
     3841 atgccggcga cgctggcctg tgcccgcgcg ggcgtgacca ccggcgagtg gggcggcacc
     3901 ctgcgcgagg tcttcggcga gtaccgcgcg ccgaccggcg tagagcacgc cgcgggcgcg
     3961 atcgaggacg accgcctgac cgagctgcgc gagaaggtcg aggcggtctc ggagacgctc
     4021 gggcggcgcg tgaagatcct ggtcggcaag ccggggctcg acggccactc gaacggcgcc
     4081 gagcagatcg ccgtgcgcgc gcgcgacgcc ggcatggacg tcgtctacga gggaatccgc
     4141 ctcacgcccg cccggatcgc cgccacggcg gtccaggagg gcgtgcacgt ggtggggctc
     4201 tcgatcctct cgggggcgca ccgcgagctg atcccggacg tgctccgcga gctgagcgac
     4261 gcaggtgcgg ccgtgccggt cgtggtgggc gggatcatcc cgcctgccga cgagaaggcg
     4321 ctgctcgagg cgggcgtcgc gcgggtctac acgccgaagg acttcgagat cacgcgcatc
     4381 atgggcgaca tcgtggagct cgtcgccgag catcacggcg ccgccgccgc ggcggcttga
     4441 cggaccccgc ggacctcggt cagcgcctcc gcgacggcga ccgatccgcg gcgccggccg
     4501 ccctgaacgt catcgaggac cgctcgccgg ccgggcgcga gcgtgcgcgg atactgctgg
     4561 cggaggtggc gccggcccgg ctcggcggcg aggcgggcgc gcacctgatc ggcgtgaccg
     4621 ggccgccggg ggccggcaag tcgtcgctgc tgtcggcgct cgtcgcgcac tggcgcgagg
     4681 cggaccgcag cgtggccgtg ctcgccgtgg acccgtcgtc gaagcgctcg ggcgggtcgc
     4741 tgcttggcga ccgggcgcgg atcgagcacg acccgcgcga cgacggcgtg ctgatccgct
     4801 ccacggccgc cggcacgcgc ctcggagggc tcgccgcgcc cacgcgcgag gccgcccagg
     4861 tgctggcccc tgcctacgac ctggttgtgg tcgagacggt gggcgtcggc cagtccgaga
     4921 ccgacgtgga ggagctctgc gacacgaccg ccgtggtggt gcaacccgcg tcgggcgaca
     4981 cgctccagtt catcaaggcg gggatcatgg aggtgcccga cgtgctggtc gtgaccaagg
     5041 ccgacctcgg cgactcggcc cgccgcgcgc ggcgcgacct gatcgccgcg ctgcgtgcac
     5101 tcggctcgag tggcacgccg gtggtcagcg tctcctccac acccccgccg cgcggcatcg
     5161 aggagctggc cgc
//
LOCUS       DASVPL010000097         6134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_128759, whole genome shotgun sequence.
ACCESSION   DASVPL010000097 DASVPL010000000
VERSION     DASVPL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128759"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VF517_04510"
     CDS             <1..1062
                     /locus_tag="VF517_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052232.1"
                     /translation="TFEVWADVARLYKLDPDWTAVSGYSMGGFGTFRMLARWPDLFAR
                     GMSTVGAPGSVSDQLVSLRNTPLMTWNSAGDELVNIRTSEQTVTDMTAAGIRFNHWLF
                     PGADHLTLSTNDQYAPAAEFLGEHRVDRDPPHVSYVIDTGEDSSRAEVVADHAYWVSG
                     LTLRDGRAVRGVFDVRSAGFGVGDPPVLDIETSQGTLEGGAHGPMPYQRRERRWGPAP
                     PLARTDKLFVKAGNVRSATIDGTRARVGCAPKVYLAIDGPLSLRLTCADKVRSKPPRC
                     TKRVSLTLPRVAARPNVAVTAGRDGKLLARATGKRVLRIAFRRPTRKAFTVEVAASSE
                     GATPGVGLTIARRVGACRR"
     gene            1076..2065
                     /locus_tag="VF517_04515"
     CDS             1076..2065
                     /locus_tag="VF517_04515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX8052233.1"
                     /translation="MRAVQITALSGPSEALELVDVPEPQEPSHMMTPGSGVVVEVHAA
                     GVSFPEVLQSRGEYQVKPDVPFVPGSEVAGVVRSAPEGSGFTAGQRVAAFCMLGGFAE
                     VAVSPEWLTFPLSDELDYAQGAGLILNYHTAYFALKLRGRLAEGETVLVHGAAGGVGT
                     ASIQVAHGLGARAIAVVSSEEKERVAREAGADEVVRSDGPWKDQAKELSGGGVDLVLD
                     PVGGDRFTDSLRSLAEGGRAVVVGFTGGSIPEVRVNRLLLKNTEVIGAGWGAYVLGKP
                     DVNREIGAAVNRMVDDGVVRPLVGARFPLERASEALELIDGRGATGKVVLDVR"
     gene            complement(2073..2555)
                     /locus_tag="VF517_04520"
     CDS             complement(2073..2555)
                     /locus_tag="VF517_04520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX8052234.1"
                     /translation="MRLVPAELRLLDAALESDADLARELGGIEVAPGWNSFPEALRAT
                     RDAVAADPESVAWGTRLFVDGETLVGWGGFKGAPAKGVVELGYEIAPEHQGRGLATDA
                     VRELIVEAHAAGEVRSVVAHTLPEHNASTRVLEKNGFVHDGEVEDGEDGTVWRWRLRR
                     "
     gene            complement(2552..4021)
                     /locus_tag="VF517_04525"
     CDS             complement(2552..4021)
                     /locus_tag="VF517_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8052235.1"
                     /translation="MTDAPKLQERRWLALALLATAQFVVVLDASIVNVALPTIGEALD
                     FSQSNLAWVVNAYVLTFGGFLLLGGRVADLMGRRRVFMGGLVLFALASLAGGLSETEG
                     QLIAARAVQGLGAAILSPAALSIVTTTFQDGAERNRALGVWGAVAGSGGAAGVLLGGV
                     LTDGLGWEWVLWVNVPIGLIAAALAPALIIESRSQSETRHFDVLGAATVTAGLSILVY
                     ALVDAVDAGWGSTQTIGLLALAVALLAVFVAIELRSRAPLVPFRIFRLRTLTGANIVG
                     LLIGASLFSMFFFISLYMQQVLGYSAIKAGLSYLPLAITIILSAGIASQLVTRVGFKP
                     VLAGGLTSIALGLLWFSQVSVGGSFGSDVLGPSVLAALGLGFSFVPVTIAAVAGVSDR
                     EAGLASGLINTSQQVGGALGLAILATVANTRTEDLMAGGGGADRTAALNEGFQSAFLT
                     GSGIAVLGIIATLTLIRTSDSRAHVELEGAGAEPVRAEA"
     gene            4077..4823
                     /locus_tag="VF517_04530"
     CDS             4077..4823
                     /locus_tag="VF517_04530"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX8052236.1"
                     /translation="MPHRRTYETDAIRVHWDSARCIHTGICLRALPEVFDLDRRPWVD
                     VEGADADAIAATVERCPTGALRYERLDGAPGDEPRRPTVALPIPDGPVLMVGDLLVES
                     PDGEEIAHENRLTLCRCGLSRNQPFCDNSHMRRRWESGEVNAPAKDYPPPREGAAEGP
                     TKVVPLEDGSLCLSGHVRVHHPDGEQLLEAGRTFLCRCGQSANKPFCDGSHERAEFRT
                     RAPEAPRDRLEAETPAAFTPNPRVPDPRGV"
     gene            4908..5237
                     /locus_tag="VF517_04535"
     CDS             4908..5237
                     /locus_tag="VF517_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8052237.1"
                     /translation="MNAADALRAARLRAGLTQHALAARAGTSQATISAYEAGRKQPSL
                     RTLERLLAATGARLEVVPDGVAAREPTPEQLARAGRTLLDVLELAAALPVRHRPTLDF
                     PRLPRSA"
     gene            5234..5791
                     /locus_tag="VF517_04540"
     CDS             5234..5791
                     /locus_tag="VF517_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052238.1"
                     /translation="MNLPERLVALHDALAAASVPHAFGGAIALAYWTLDPRGTSDIDC
                     NLFVPAAEPGPALDALPDGIRRPPGLADVIARDGQVRLWWDETPVDLFFDYEPVHEQA
                     ARNSRRVPFASTEIPILGPVELVVFKVMFDRTRDWADVEAVIEAGRVDLRAVRDTLRT
                     LLGPGDPRLDRLAETERRVRPEGVA"
     gene            5788..>6134
                     /locus_tag="VF517_04545"
     CDS             5788..>6134
                     /locus_tag="VF517_04545"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX8052239.1"
                     /translation="MSAIDIDTPHGLARAHLHSASDPRGALVLGHGAGGGVQAPDLVA
                     VTGTATAVGFSVALVEQPYRVAGRRSAAPAHQLDASWIAAVEQLGTGALRRLPLVAGG
                     RSSGARVACRTAAA"
BASE COUNT          820 a         2302 c         2263 g          749 t
ORIGIN      
        1 acgttcgagg tctgggccga cgtcgcgcgc ctgtacaagc tcgacccgga ctggaccgcg
       61 gtgagcggct actcgatggg cgggttcggc accttccgca tgctcgcgcg ctggcccgac
      121 ctgttcgccc gcggcatgtc cacggtcggc gcgcccggca gcgtctccga ccagctcgtc
      181 tcgctgcgca acacgccgct catgacctgg aactccgccg gcgacgagct cgtgaacatc
      241 cggaccagcg agcagacggt caccgacatg accgccgcgg ggatccggtt caaccactgg
      301 ctcttccccg gcgccgacca cctgacgctg tccacgaacg accagtacgc gccggccgcc
      361 gagttcctcg gcgagcaccg cgtcgaccgc gacccgccgc acgtgagcta cgtgatcgac
      421 acgggcgagg actcgtcccg ggccgaggtc gtggccgatc acgcctactg ggtctccggg
      481 ctcacgctgc gcgacgggcg cgccgtgcgc ggcgtgttcg acgtgcgctc ggccggcttc
      541 ggcgttggcg acccgccggt gctcgacatc gagacctcgc aggggacgct ggagggcggc
      601 gcccacgggc cgatgccgta ccagcgccgc gagcgccgct ggggcccggc gccgcccctc
      661 gcgcgcaccg acaagctgtt cgtgaaggcc ggcaacgtgc gctcggccac catcgacggc
      721 acccgcgcgc gggtcggctg cgcgccgaag gtctacctcg cgatcgacgg cccgctctcc
      781 ctgcgcctca cctgcgcgga caaggtgcgc agcaagccgc cgcgctgcac caagcgcgtc
      841 tcgctcaccc tgccgcgcgt ggccgcgcgc ccgaacgtgg cggtcaccgc cggccgcgac
      901 ggcaagctgc tggcgcgcgc gaccggcaag cgcgtgctgc ggatcgcgtt caggcggccc
      961 acgcgcaagg cgttcacggt cgaggtggcc gcgagctcgg agggcgccac gccgggggtg
     1021 gggctgacga tcgcgcggcg cgtcggggcc tgccgccggt agggtccggc ccctgatgag
     1081 ggccgtgcag atcaccgcgc tgagcgggcc gtccgaggcg ctcgagctcg tggacgtccc
     1141 cgagccgcag gagccctcgc acatgatgac ccccggcagc ggcgtggtcg tagaggtcca
     1201 cgcggccggg gtctcgttcc cggaggtgct ccagtcgcgc ggcgagtacc aggtcaagcc
     1261 cgacgtgccg ttcgtgcccg gcagcgaggt ggccggcgtc gtccgcagcg cccccgaggg
     1321 cagcgggttc accgccggcc agcgggtggc cgccttctgc atgctcggcg gcttcgccga
     1381 ggtggcggtg tcgcccgagt ggctcacctt cccgctctcc gacgagctcg actacgccca
     1441 gggcgcgggc ctcatcctca actaccacac ggcctacttc gcgctgaagc tgcgcgggcg
     1501 gctggcggag ggggagaccg tgctggtgca cggcgcggcc ggcggcgtcg ggaccgcctc
     1561 gatccaggtc gcgcacgggc tgggcgcgcg ggcgatcgcc gtcgtgtcga gcgaggagaa
     1621 ggagcgggtc gcgcgcgagg cgggcgccga cgaggttgtg cgctccgacg ggccctggaa
     1681 ggaccaggcc aaggagctct cgggtggggg agtggacctg gtgctcgacc ctgtgggcgg
     1741 cgaccgcttc accgacagcc tgcgctcgct ggccgagggc gggcgcgccg tggtggtggg
     1801 cttcaccggg ggctcgatcc cggaggtgag ggtcaaccgc ctgctgctga agaacaccga
     1861 ggtgatcggc gccggctggg gcgcctacgt gctcggcaag cctgacgtga accgcgagat
     1921 cggcgccgcc gtgaaccgga tggtcgacga cggcgtggtc cgcccgctcg tcggcgcgcg
     1981 cttcccgctg gagcgcgcga gcgaggcgct cgagctgatc gacggccgcg gggcgaccgg
     2041 caaggtcgtc ctcgacgtgc gctgagcgag cctcagcgcc gcaggcgcca gcgccagacg
     2101 gtcccgtcct cgccgtcctc gacctcgccg tcatggacga agccgttctt ctccagcacg
     2161 cgggtcgatg cgttgtgctc ggggagcgtg tgcgccacga cgctgcgcac ctcgccggcc
     2221 gcgtgcgcct ccacgatcag ctcgcgcacc gcgtcggtcg cgaggccgcg gccctggtgc
     2281 tccggcgcga tctcgtagcc gagctcgacg acccccttcg ccggcgcgcc cttgaagccg
     2341 ccccaaccga cgagcgtctc gccgtccacg aacagccgcg tgccccacgc gacggattcg
     2401 gggtcggcgg ccacggcgtc gcgcgtggcc cgcagcgcct ccgggaacga gttccagccg
     2461 ggcgcgacct cgatcccgcc cagctcgcgc gccaggtcgg cgtcggactc gagcgcggcg
     2521 tcgagcagcc gcagctcggc cgggacgagc ctcacgcctc ggcgcggacc ggctcggcgc
     2581 cggcgccctc gagctccacg tgcgccctgc tgtcgctggt ccgtatcagc gtcagcgtcg
     2641 cgattatgcc gagcaccgcg atgccggagc cggtcaggaa ggcggactgg aagccctcgt
     2701 tcagcgcggc ggtgcggtcg gcgccgccac ccccggccat gaggtcctcg gtgcgcgtgt
     2761 tcgcgacggt cgcgaggatc gccagcccga gcgcgccgcc cacctgctgc gaggtgttga
     2821 tgagcccgct ggcgaggccg gcctcgcggt cggacacccc ggccaccgcg gcgatggtga
     2881 cgggcacgaa cgagaagccg agcccgagcg cggcgagcac ggacggcccg agcacgtcgc
     2941 tgccgaagct tccgccgacc gagacctgcg agaaccagag cagcccgagc gcgatcgacg
     3001 tgagcccgcc ggcgagcacc ggcttgaagc ccacgcgcgt gacgagctgc gacgcgatcc
     3061 ccgccgacag gatgatcgtg atcgcgagcg gcaggtacga cagccccgcc ttgatcgcgc
     3121 tgtagccgag cacctgctgc atgtagagcg agatgaagaa gaacatcgag aagagcgacg
     3181 cgccgatcag cagcccgacg atgttcgcgc cggtgagcgt ccgcaggcgg aagatgcgga
     3241 acgggacgag cggggcgcgg gagcgcagct cgatcgcgac gaagaccgcg agcagcgcca
     3301 ccgcgagcgc gagcagcccg atcgtctgcg tcgagcccca accggcgtcg accgcgtcca
     3361 ccagcgcgta gaccaggatc gacaggccgg cggtgacggt ggcggcgccc agtacgtcga
     3421 agtggcgcgt ctccgactgc gatcggctct cgatgatcag cgcgggcgcg agcgcggcgg
     3481 cgatgagccc gatcggcacg ttcacccaca gcacccactc ccagccgagg ccgtcggtga
     3541 gcacgccgcc gagcagcacc ccggccgcac cgccgctgcc cgcgaccgcg ccccacacgc
     3601 cgagggccct gttgcgctcg gcgccgtcct ggaacgtggt ggtgacgatc gagagggcgg
     3661 cgggggagag gatcgcggcg ccgaggccct ggaccgcgcg cgcggcgatg agctgaccct
     3721 cggtctcgga taggccgccg gccagcgagg ccagcgcgaa caggaccagc ccgcccatga
     3781 acacgcgccg gcgccccatc aggtcggcca cgcgcccgcc gagcagcagg aagccgccga
     3841 aggtgagcac gtacgcgttc acgacccagg ccaggttgct ctgcgagaag tcgagcgcct
     3901 cgccgatcgt cgggagcgcg acgttcacga tcgacgcgtc gagcacgacc acgaactggg
     3961 cggtggcgag cagtgcaagg gcgagccagc ggcgttcttg gagcttgggg gcgtcggtca
     4021 tgtctcctct tcgacgttat cgaggcccgt tcggcccgtt tgatgcaata cgtcacgtgc
     4081 cccaccgccg gacctacgag accgatgcga tccgcgtgca ttgggactcc gcccgctgca
     4141 tccacaccgg gatctgcctg cgcgcgctgc cggaggtgtt cgacctcgac cggcgcccgt
     4201 gggtggacgt cgagggcgcc gatgccgacg cgatcgccgc gacggtcgaa cgctgcccga
     4261 ccggggcgct gcgctacgag cgcctcgacg gcgcgcccgg cgacgagccg cgccggccca
     4321 cggtggccct cccgatcccg gacgggccgg tcctgatggt cggcgacctg ctggtcgaga
     4381 gcccggacgg cgaggagatc gcgcacgaga accggctgac cctctgccgc tgcgggctga
     4441 gccgcaacca gccgttctgc gacaactccc atatgcgccg ccgctgggag tccggcgagg
     4501 tgaacgcgcc cgccaaggac tacccgccgc cgcgcgaggg cgccgccgag gggccgacga
     4561 aggtggtgcc gctcgaggac ggctcgctct gcctgagtgg gcacgtgcgc gtgcaccacc
     4621 ccgacggcga gcagctcttg gaggccggtc gcacgttcct gtgccgctgc gggcagagcg
     4681 cgaacaagcc gttctgcgac ggcagccacg agcgggcgga gttccgcacc cgcgcgccag
     4741 aggcgccgcg cgaccggctc gaggccgaga cgccggcggc gttcacgccg aacccgcgcg
     4801 tgccggaccc gcgcggcgtc tgaggcgcgg ggggagccgc ccggccgccg cgcgcggctt
     4861 cgccgccggc ctcgccagcc gatatccgca gggatatact cccggacgtg aacgctgcgg
     4921 acgcgctccg cgccgccagg ctgcgcgcgg ggctcacgca acacgcgctt gccgctcgcg
     4981 ccgggacctc gcaggcgact atctcggcgt acgaggccgg ccgcaagcag ccgtcgctgc
     5041 gcacgctcga gcgactcctc gccgccaccg gggcgcggct cgaggtggtg cccgacggcg
     5101 tcgccgcgcg cgagccgacg cccgagcagc tcgcgcgcgc cggccgcacg ctgctcgacg
     5161 tgctcgagct ggccgcggcg ctgcccgtgc gccaccggcc gacgctcgac ttcccgcgcc
     5221 tgccgcgctc ggcatgaacc tcccggagcg cctcgtcgcg ctccacgacg cactcgcggc
     5281 ggccagcgtg ccgcacgcgt tcggcggagc gatcgccctg gcctactgga cgctcgaccc
     5341 gcgtggcacg agcgacatcg actgcaacct gttcgtgccc gccgcggagc ccggccccgc
     5401 gctcgacgcg ctgccggacg ggatccgccg accgcccggc ctggccgatg tcatcgctcg
     5461 tgacgggcag gtgcgcctct ggtgggacga gaccccggtc gatctcttct tcgactacga
     5521 gccggtgcac gagcaggccg cgcggaacag ccgccgcgtc ccgttcgcga gcaccgagat
     5581 cccgatcctc ggaccggtcg agctggtcgt gttcaaggtg atgttcgacc gcacgcgcga
     5641 ctgggcggac gtcgaggcgg tgatcgaggc cggcagggtg gacctgcgcg ccgtgcgcga
     5701 caccctgcgc acgctgctcg gccccggcga cccgcgcctc gaccgcctgg ccgagaccga
     5761 gcggcgcgtg cgcccggagg gcgtcgcgtg agcgcgatcg acatcgatac cccgcacggc
     5821 ctcgcgcgcg cccacctgca ctcggcgagc gacccgcgcg gcgcgctcgt gctgggtcac
     5881 ggcgcgggcg ggggagtgca ggccccggac ctcgtcgcag tgaccggcac cgccaccgcg
     5941 gtcgggttca gcgttgcgct ggtggagcag ccgtaccgcg tcgcgggccg gcgctcggcg
     6001 gcgcccgcgc atcagctcga cgcctcctgg atcgccgccg tcgagcagct cgggaccggc
     6061 gccctgcgcc gcctgccgct cgtggccggc ggccgctcgt ccggcgcgcg cgtggcgtgc
     6121 cgcacggccg cggc
//
LOCUS       DASVPL010000098         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_128864, whole genome shotgun sequence.
ACCESSION   DASVPL010000098 DASVPL010000000
VERSION     DASVPL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128864"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VF517_04550"
     CDS             <1..1368
                     /locus_tag="VF517_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX8052240.1"
                     /translation="DARRSRLEDLLFGSILSVEGLDLALAAAVAMLAAALVAGAGKEL
                     ALAAFDRRMARAMGYRLRVLDLALFGAVALAAIVALRAVGNVLVAALLLGPPLIARHV
                     CRSFASMCAASGAIGAAAGVGGLYWSWYENVGAGAAIVLVIAALYALVATASLALRLR
                     PVAPAAALTALAAAALLAGCGDSGDSGKPRVVATTMQLADIARQVGGDRVQVDGILGP
                     DSEPHEYEPKPSDADAVADADLVLQNGGGLDDWLDGLLDSAGGDAERVTIVGDGARPA
                     VADGGDPHVWHDPHAAKLMVDAVERGLAQVAPNAAARIHANATRYRARIDRMAREIRA
                     LLAPIPRERRKLVTSHDAFGHFARVYGIDVVGSVLPSVSTDTEPSGEQVRQLVDAIRR
                     AGVDTIFTERAVNAKLERQVAAEAGAQVSTSLYADTLGEPGTPAATLIGAELLNARAM
                     AEAWR"
     gene            1365..1805
                     /locus_tag="VF517_04555"
     CDS             1365..1805
                     /locus_tag="VF517_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX8052241.1"
                     /translation="MKGAAWREHTETRLRAAGYRAGGGRRAVIEVLARHGCLLTANAI
                     LAELRDERPGIGFATVYRALETLTDLGLVRRVDAGTGSATYEPVDPSGEHHHHVVCDR
                     CGYVTAFADDELERTIEQLSRRLGHRIESHEVVLRGPCRDCRSR"
     gene            1836..>2928
                     /locus_tag="VF517_04560"
     CDS             1836..>2928
                     /locus_tag="VF517_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052242.1"
                     /translation="MDELVAYERRFRKAGLPLFIEDYSAAGDVFNRAIPLLGLVFVVE
                     LLGAIDLDWAWYANVAAAFGGLVVLLTATGLVNRMRGRPFLSVPERLGVPELTTFVLV
                     PALLPLIFSGQVESAIVTAAGNLLLLLAIYLVVGYALPAILREAAIGLATELANAVVT
                     LTRALPLLLLFAVVLFVNTEMWQVLSNTPDALLWLLGALLIGLGTLFLVARLPQEVRE
                     LESAAPDGPPLNRRQRINVALVVLVSHALQVIVVAAGLGAVFVVFGALAVGPEVREAW
                     IGSTGDSLFALDVLGERVEVTAELLRVSGGVAALSGLYYAIAVLTDATYREQFLDRLS
                     TEMCASFEARAEYLRLRERLSGDALAPRPR"
BASE COUNT          335 a         1106 c         1061 g          426 t
ORIGIN      
        1 gacgcgcgcc gctcgcggct cgaggacctg ctgttcgggt cgatcctgag cgtcgaagga
       61 ctcgacctcg cgctggcggc cgccgtggcc atgctcgccg ccgcgctggt cgcgggcgcg
      121 ggcaaggagc tggcgctcgc ggcgttcgac cgtcgcatgg cgcgcgccat gggctaccgg
      181 ctgcgcgtgc tcgatctcgc gctgttcggc gccgtcgcgc tggcggcgat cgtggcgctg
      241 cgcgccgtcg gcaacgtgct ggtcgcggcg ctgctgctcg gcccgccgct gatcgcccgt
      301 cacgtctgtc gctcgttcgc ctcgatgtgc gcggcgagcg gcgccatcgg cgcggccgcc
      361 ggggtgggcg gcctgtactg gtcctggtac gagaacgtcg gggccggagc ggcgatcgtg
      421 ctcgtgatcg ccgccctgta cgcgctggtc gcgaccgcct cgctcgcgct gcggcttcgc
      481 cccgtggctc ccgccgcggc cctgacagcg cttgccgcgg cggcgctcct cgccggctgc
      541 ggcgactccg gcgactcagg caagccgcgc gtggtggcca ccactatgca gctcgcggac
      601 atcgcgcgtc aggtgggcgg cgaccgcgtg caggtggacg ggatcctcgg ccccgactcc
      661 gagccgcacg agtacgagcc gaagccgtcg gacgccgacg ccgtggcgga cgccgacctg
      721 gtcctccaga acggcggcgg gctcgacgac tggctcgacg gcctgctgga cagcgcgggc
      781 ggcgacgccg agcgggtcac gatcgtcggc gacggcgccc ggccggccgt cgcggacggc
      841 ggcgatcccc acgtctggca cgacccgcac gccgccaagc tcatggtcga cgccgtggag
      901 cgcggcctcg cccaggtcgc cccgaacgcg gccgcacgca ttcacgcgaa tgcgacccgc
      961 taccgcgcgc ggatcgaccg catggcgcgc gagatccgcg ccctgctagc gccgatcccg
     1021 cgcgagcgcc gcaagctcgt tacgagccac gacgcgttcg gccacttcgc gcgtgtgtac
     1081 gggatcgacg tggtcgggag cgtgctgccg agcgtcagca ccgacaccga gccgtcgggc
     1141 gagcaggtgc gccagctggt cgacgccatc cgccgtgccg gggtcgacac gatcttcacc
     1201 gagcgggccg tgaacgcgaa gctcgagcgc caggtcgcgg cggaggccgg cgcgcaggtg
     1261 tcgacctccc tctacgcgga cacgctcggc gagcccggca cgccggcggc cacgctgatc
     1321 ggggcggagc tgctcaacgc acgcgcgatg gccgaggcgt ggcgatgaag ggcgccgcct
     1381 ggcgcgagca caccgagacc cgcctgcgcg cggccggcta ccgcgcgggt ggcggccggc
     1441 gggcggtgat cgaggtcctg gcccgccacg gctgcctgct caccgcgaac gcaatcctcg
     1501 ccgagcttcg ggacgagcgt ccgggcatcg gcttcgcgac cgtctaccgc gcgctcgaga
     1561 cgctcaccga ccttgggctc gtccggcgtg tggacgccgg caccggcagc gccacctacg
     1621 agccggtgga cccgagcggc gagcaccacc accacgtcgt atgcgaccgc tgcggctacg
     1681 tgaccgcctt cgccgacgac gagctggagc gcaccatcga gcagctcagc cgccgcctcg
     1741 gtcaccggat cgagagccac gaggtcgtgc tgcgcggccc ctgtcgcgac tgccgctcgc
     1801 gctgacgtcc gtagcgcccg ctcgaatacc gggcgatgga cgagctcgtc gcctacgaac
     1861 gccgcttccg caaggcgggg ctcccgctgt tcatcgagga ctattcggcc gccggcgacg
     1921 tcttcaaccg ggcgatcccg ctgctcggac tcgtgttcgt ggtcgagctg ctcggcgcga
     1981 tcgacctcga ctgggcctgg tacgccaacg tcgcggcggc gttcggcggg ctcgtcgtgc
     2041 tgctcacggc caccggcctg gtcaaccgca tgcgcggccg cccgttcctc tcggtgcccg
     2101 agcgcctcgg cgtgcccgag ctgaccacgt tcgtgctcgt ccccgcgctg ctgccgctga
     2161 tcttcagcgg ccaggtcgag agcgcgatcg tcacggccgc cggcaacctc ctcctcctgc
     2221 tcgcgatcta cctggtcgtg ggctacgcgc tcccggcgat cctgcgcgag gccgccatcg
     2281 gactcgccac cgagctggcc aatgccgtcg tcacgctcac gcgcgcgctc ccgctgctcc
     2341 tcctgttcgc ggtggtgctg ttcgtgaaca cggagatgtg gcaggtgctc tccaacacgc
     2401 cggacgcgct cctctggctg cttggcgcgc tgctgatcgg cctcggcacc ctgttcctgg
     2461 tcgcgcggct cccgcaggag gtgcgcgagc tcgagtcggc cgcgcccgac ggcccgccgc
     2521 tcaaccgccg ccagcggatc aacgtcgcgc tggtcgtgct cgtgagccac gcgctccagg
     2581 tgatcgtcgt ggccgccggg ctcggcgccg tgttcgtggt gttcggcgcg ctcgcggtcg
     2641 gcccggaggt gcgcgaggcg tggatcggct cgaccggcga ctcgctgttc gcgctcgacg
     2701 ttctcggcga gcgcgtggag gtcacggccg agctgctgcg cgtctcgggc ggggtcgccg
     2761 cgctcagcgg gctctactac gcgatcgccg tgctcaccga cgcgacctac cgtgagcagt
     2821 tcctcgaccg gctgagcacc gagatgtgcg cgagcttcga ggcccgcgcc gagtacctgc
     2881 ggctgcgcga gcggctctcc ggcgacgcgc tcgcgccccg gccgcggg
//
LOCUS       DASVPL010000099         9079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_286514, whole genome shotgun sequence.
ACCESSION   DASVPL010000099 DASVPL010000000
VERSION     DASVPL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286514"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VF517_04565"
     CDS             <1..419
                     /locus_tag="VF517_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8052243.1"
                     /translation="ARLGGGTWSGGERVRCSSTRELSRALVELNMGRPDQRPLAGRVV
                     DALVPRVRDIRRGGSAAAALAQVATGRAEGYWGPGLRAWDGAAGLLLVTEAGGIVGDL
                     AGSSPARWPASGDVLAAAPALFEPLRSALRAALPSP"
     gene            433..1272
                     /locus_tag="VF517_04570"
     CDS             433..1272
                     /locus_tag="VF517_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052244.1"
                     /translation="MPDPPQQQTPPGSDERLDPQADHGEQSYRGSGRLAGKAAIVTGA
                     DSGIGKAVAIAYAREGADVLISYLDEHDDARDTQRWVEEAGRRAVLIAGDLAEPAHCR
                     EIVAKAVEEFGRVDVLVSNAAYQMTRDSLEEIPDEEWDHTVAVNLSAMFHLCKAAVPH
                     MPRGGSIIGSTSINSDQPLPTLLPYDVTKAGIANFIAAAAQMLAERGIRANSVAPGPI
                     WTPLIPSTMPPDQVETFGEDVPLGRPGQPAECAPVYVMLASDEASYVSGARVAVTGGK
                     PIL"
     gene            complement(1269..2144)
                     /locus_tag="VF517_04575"
     CDS             complement(1269..2144)
                     /locus_tag="VF517_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8052245.1"
                     /translation="MRIATWNVNSAKQRVPRLLPWLDERRPDVVCLQETKLADEAFTD
                     LLGEELADRGYAVALHGEPSWNGVAILSRVGLDDVVAGIPGGPGFPHPEARAVSATCD
                     GVRVVSVYVPNGRAPDSEHYHYKLAWLAALRDLIGADPDTTVVAGDMNIAPTDDDVFD
                     PEAYVGHTHVTPPERAALAELQALGLRDVVRERWPDERVFTYWDYRAGMFHQDLGMRI
                     DLVLAGGAVADRVRAAWVDRHARKGSGPSDHAPVIVDLDEAPDGDIGPVVPPPSAPAR
                     RRGSRKLPQSPEPPG"
     gene            2250..4172
                     /locus_tag="VF517_04580"
     CDS             2250..4172
                     /locus_tag="VF517_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052246.1"
                     /translation="MGSRPTKPTFQGVVDLGLRDVGVRNEPRRLAIDFGKGSLQAFAL
                     VFLSSVAMCAIVAGTGAGGWIAIPFALTFTGAVLALGLRSARRSPMMVVRVTAEGTNQ
                     AAWMMMVPLVLLALLALISGSPLMVPLLVLPAAMAALVLRTRGYVPRALRRLRPLLGA
                     DEPVLGDGVGRADGVRGWHNAFRLVVATDRRLLVATSTRSTERFPLLDVPYDRVSRFG
                     IEWRQLGRVGVLSLTVTGAGGAPSETHVIGSIAPANLLSIAEALQSNGVQADDPATVS
                     SARLAWDEARAAASARRGRAAEPRTPRTRLLDRADVNTAAFDRGLWLLLALTAFAFYV
                     NPFGVGLGAGRDPDLAVLALVPVVCGVSSYVSGTRSALAYAVPLNLLAAPAFFFAPAG
                     YVIALMVVMSAVAAAGLWAGSALRRATGGPVDAGAGKRAVRGSLRYTLGGAGLVRITG
                     VMLAGMLAIVATAGAAGFELTSLRLAIDEATLEQVPVDGRSNLTGDVASVTYTTGPDL
                     HEFITDQPTGGGVTGAARWELRSSFTKGRNVVSLARYTYEPALDDPAAVADFVAEKDR
                     EHSRLAGSDVTHSERVVDGRRGYVWNHRSPYGYWYYAAWFPQRVHTVRVECIAKQQEA
                     RFKRLCAEAVGSLEFR"
     gene            complement(4169..6190)
                     /locus_tag="VF517_04585"
     CDS             complement(4169..6190)
                     /locus_tag="VF517_04585"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8052247.1"
                     /translation="MEDPRVSRRELLAAAAAAAAALALRDPAGAAAAGWTGGIDFGSA
                     GAGEGWPKWRCAGVANLRRSNGVGVLEAGSDVFPNDPRPVAFAVDRRFRDGEISASVD
                     AAGAGAGLVLRRTGPRDYYAAVYDDEQAALLLLRRSPAGLSELARTALARPSTPFRLA
                     LTASGANPTTLAAALDSGGAPVTVAAADSAAALQRAGDPGVLATARTLFPAEGPGPAA
                     ALGNLHLLPYGVQEGQAVIGSPAGAGVMEAIRERSTAAFTRIDLRAAERPAPTPASVV
                     AASGGTPVTGGATLRVAADVPARVEIEIADNSAFRRSRRIRPARTNGFDAAFAKATGL
                     RQGRRVYWRARLRRRGLETVGPARSFRALPRAGSGKHATIAIGSCASQFGPIFDELAR
                     REPDLFVWQGDLNYPDTVGPLAQTVSGYGGMWRELLANPRLAPILERSLFATQRDDHD
                     YGLQDANSTNLVPWGLAPWEALMEPRVHHRFSAGLADFWILDQRLHKTDPAAPDTPDK
                     TLLGAAQRDWLLRTLTASRAPFKVVCSPCTLAPLKANERDGSWAAGYTAERDLIRAHI
                     AERVPGRTLFVTGDTHWTMVYERDGLFEARPCPLGIPTPNDITLTQPNVAEDARGVPG
                     VAYADDERGHFALLEVRRDGATAHLDLTLVRDDGATPFSTRFEQPRVRT"
     gene            complement(6194..7090)
                     /locus_tag="VF517_04590"
     CDS             complement(6194..7090)
                     /locus_tag="VF517_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8052248.1"
                     /translation="MRAGIVILPEQRWADARARWTRAEEYGFAHAWTYDHLGWRDLVE
                     GPWFDSIVTLTAAAGATESIRLGTLVASPNFRHPVHFAREITALDDISGGRLVLGLGA
                     GAAAPSFDALVLGQPELSPGERVDRFAEFVELLDLLLREQRVTWRGSYYEAVDARTTP
                     GCVQSPRVPFVVAANGRRSMGLAARFGEGWITMGGSADDLESWWRSVADRAARFEGPG
                     DRYLMLDSAPRFSLSSRAFFEEQVGRAAELGFTDVITHWPRPSSWYAGDEAVLEEAAA
                     EVLPHLAPRGAPPGMLGEMGES"
     gene            complement(7134..7556)
                     /locus_tag="VF517_04595"
     CDS             complement(7134..7556)
                     /locus_tag="VF517_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8052249.1"
                     /translation="MSYTVLNAADAFWRPSNLMGVLNTDLAGQLGASTFGARMWRLAP
                     GQASTRHRHPDQHELYVVLEGTGRVRIGDETLTLAPHSALLVEPATVRQVFNDTDADA
                     LWLVVGAPPEALDTQTMTAEQLDWIYPEGPRVLPPELA"
     gene            7689..8054
                     /locus_tag="VF517_04600"
     CDS             7689..8054
                     /locus_tag="VF517_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8052250.1"
                     /translation="MPRRKPGTLLPLEIEILEAALSMPASGQRGFHGFGLAQTMREQS
                     GAQSLTAHGTLYKALSRLEEFGLLTSRWEDAASAEGRPRRRLYELTGAGAQAAERARA
                     GRAPAKAVGRSPRVASRSA"
     gene            8051..8839
                     /locus_tag="VF517_04605"
     CDS             8051..8839
                     /locus_tag="VF517_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052251.1"
                     /translation="MRPERMARLVARWVRIYTRDLPDPLGRRRAGEIDADLHDHIAHE
                     RAHGTSDGRIALSVLSRMLRGLPADASWHRRHAEARRTVLRSAVRVALATSLVLLVPL
                     FAMLVTDAVAWSPFDFAAAGFLLFGAGLAYELVVRRPGAIAYRAGVGLALAAAFVLVW
                     LVGAVGVIGEDGDRADLMYGGVLAVGIVGAAVARLRPRGMARALLAMAVAQALVAAIA
                     LLAGKHQDTVSSLPELLGLNGFFVALFAASAWLFRRAAGGQPPA"
     gene            complement(8857..>9079)
                     /locus_tag="VF517_04610"
     CDS             complement(8857..>9079)
                     /locus_tag="VF517_04610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX8052252.1"
                     /translation="EFMGLSTVLVAPTSTQARPASFRPTIEIQGQETRVLVEQTTVVD
                     PQRLGGSDGRLDASEMRAVDDAMRLLLGL"
BASE COUNT         1145 a         3329 c         3403 g         1202 t
ORIGIN      
        1 aggcgcggct cggcggcggc acgtggagcg ggggcgagcg cgtgcgctgc agctccacgc
       61 gcgagctcag ccgcgcgctc gtggagctga acatgggccg ccccgaccag cgaccgctcg
      121 cggggcgcgt ggtcgacgcg ctcgtgccgc gggtgcgcga catccgccgc ggcggcagcg
      181 ccgccgccgc gctcgcgcag gtcgcgaccg gccgcgcgga gggctactgg gggcccggcc
      241 tgcgggcctg ggacggagcg gcgggactgc tgctcgtcac cgaggctggc gggatcgtcg
      301 gcgacctggc cggcagctcg cccgcccgct ggcccgcgag cggcgacgtg ctcgccgccg
      361 cccccgcgct cttcgaaccg ttgcggagcg ccctgcgcgc agccctcccc tccccctgac
      421 caggagctgc cgatgcccga cccgccccag cagcagaccc cgcccggcag cgacgagcgg
      481 ctcgacccgc aggcggatca cggcgagcag agctacaggg ggtccgggcg cctggccggc
      541 aaggcggcga tcgtgacggg cgccgacagc ggcatcggca aggcggtggc gatcgcgtac
      601 gcccgcgagg gcgccgacgt gctgatctcc tacctcgacg agcacgacga cgcgcgcgac
      661 acgcagcgct gggtcgagga ggcaggccgg cgcgcggtgc tcatagccgg cgacctggcg
      721 gagccggcgc actgccgcga gatcgtggcg aaggcggtcg aggagttcgg ccgcgtcgac
      781 gtgctggtga gcaacgcggc gtaccagatg acgcgcgact cgctcgagga gatccccgac
      841 gaggagtggg accacacggt ggcggtcaac ctctcggcga tgttccacct ctgcaaggcg
      901 gcggtcccgc acatgccgcg gggtgggtcg atcatcggct cgacgtcgat caactcggac
      961 cagccgctgc ccacgctgct cccgtacgac gtgacgaagg cgggcatcgc caacttcatc
     1021 gccgccgcgg cgcagatgct ggccgagcgc gggatccgcg cgaacagcgt ggccccggga
     1081 ccgatctgga ccccgctgat cccctccacc atgccgcccg accaggtcga gaccttcggc
     1141 gaggacgtgc cgctcggtcg ccccggtcag ccggcggagt gcgcgccggt gtacgtgatg
     1201 ctcgcgtccg acgaggcgag ctacgtctcc ggcgcgcgcg tggcggtcac cggcggcaag
     1261 ccgatcctct agccgggcgg ctccggcgat tgcggcagct tgcgcgagcc gcgcctgcgc
     1321 gcgggagcag agggcggtgg gacgacgggc ccgatgtcgc cgtcgggcgc ctcgtcgagg
     1381 tcaacgatca cgggcgcgtg gtcgctcggc ccgctgccct tgcgggcgtg gcgatcgacc
     1441 cacgccgccc ggactcggtc ggccaccgcg ccgcccgcca gcaccaggtc gatccgcatg
     1501 ccgaggtcct ggtggaacat gccggcccgg tagtcccagt aggtgaacac gcgctcgtcc
     1561 ggccagcgct cgcgcacgac gtcgcgcagg ccgagcgcct gcagctcggc gagcgcggcg
     1621 cgctcggggg gcgtcacgtg cgtgtggccg acgtaggcct cgggatcgaa gacgtcgtcg
     1681 tcggtcggcg cgatgttcat gtcgccggcc acgacggtcg tgtccgggtc ggcgccgatg
     1741 agatcgcgca gcgccgcgag ccaggccagc ttgtagtggt agtgctcgga gtccggcgcg
     1801 cgtccgttcg ggacgtagac cgacacgacc cgcacgccgt cacacgtcgc cgacaccgcg
     1861 cgggcctcgg gatgcgggaa gcccggcccg ccggggatac ccgccaccac gtcgtcgagc
     1921 cccacgcgcg agaggatcgc cacgccgttc cagctcggct cgccgtggag cgcgacggcg
     1981 tagccgcggt ccgcgagctc ctcgccgagc aggtcggtga acgcctcgtc ggcgagcttg
     2041 gtctcctgga ggcagaccac gtccgggcgc cgctcgtcca gccagggcag gagccgcgga
     2101 acccgctgct tcgccgagtt gacgttccag gtggcgatgc gcacggggca tcacgctagc
     2161 cggggcgctc cggcgctaca gggagagcac cgctcggccg atctagtgcg tgccggcggc
     2221 gggcgttgcg actgcgtggg cggatgcgag tgggatcgag accgacaaag ccgaccttcc
     2281 agggcgtcgt ggacctcggt ctgcgcgacg tcggcgtcag gaacgagccg cggcggctcg
     2341 ccatcgactt cgggaaggga tcgctccagg cattcgcgct ggtcttcctc tccagcgtcg
     2401 cgatgtgcgc gatcgtggcc ggcacgggag cgggcggctg gatcgccatc ccgttcgcgc
     2461 tgaccttcac gggcgccgtc ctcgcgctgg ggctgcggtc ggcgaggcgc tcgccgatga
     2521 tggtcgtcag ggtcacagcc gagggcacca accaggcggc ctggatgatg atggtgccgc
     2581 tcgtcctcct cgctctcctc gccctgatct ccgggagccc gctgatggtc ccgctgctcg
     2641 tcctcccggc ggccatggcc gcgctcgtgc ttcgcacgcg tggatacgtg ccccgggcgc
     2701 tgcgcaggct ccgccccctg ctgggggcgg acgagccggt cctgggcgac ggagtcggcc
     2761 gggccgacgg ggtgcgcggc tggcacaacg cgttccgcct ggtggtggcg accgaccggc
     2821 gcctgctcgt ggccacctcg acccgctcga ccgaacgctt cccgctgctc gacgtgccct
     2881 acgaccgcgt gagccgtttc ggcatcgagt ggaggcagct ggggcgcgtg ggggtgctct
     2941 cgctgaccgt gaccggagcc ggcggcgcgc cctccgagac gcacgtcatc ggctccatcg
     3001 caccggcgaa cctgctgtca atcgcggagg cgctccagtc gaacggcgtg caggcggacg
     3061 atcccgcgac cgtctccagc gcgcggctcg cgtgggacga ggcgcgggcg gcagcatccg
     3121 cgaggcgcgg ccgggcggcc gagccgcgca ccccgcgcac gcgcctgctc gaccgcgcgg
     3181 acgtgaacac ggcggcgttc gaccgcggcc tgtggctgct gctggcgctg acggccttcg
     3241 ccttctacgt caacccgttc ggcgtcgggc tcggggccgg gcgcgacccg gacctggcgg
     3301 tgctggcgct cgtgccggtg gtgtgcggag tctcgagcta cgtgtctggc acgaggtcgg
     3361 cgctcgccta cgccgtcccg ctcaacctgc tcgccgcccc cgccttcttc ttcgcgcccg
     3421 cgggctacgt gatcgccctg atggtcgtca tgagcgccgt cgcggccgcc ggcctgtggg
     3481 ccggatcggc gcttcgccga gcgactggtg ggcccgtgga cgcgggcgcc ggcaagaggg
     3541 cggtccgcgg gagcctgcgc tacacgctcg gcggcgcggg cctggtccgg atcaccgggg
     3601 tcatgctcgc cgggatgctg gcgatcgtcg cgaccgccgg cgccgccggc ttcgagctca
     3661 cctcgctgcg cctcgcaatc gacgaggcga ccctcgagca ggtgcccgtc gacggcagat
     3721 ccaacctcac cggcgacgtc gcctcggtca cctatacgac cggcccggac ctgcacgagt
     3781 tcatcaccga ccagccgacc ggcggcggcg tgaccggcgc cgcccgctgg gagctccgct
     3841 cctccttcac caagggacgc aacgtggtct cgctcgcgcg ctacacctac gagcccgccc
     3901 tggacgaccc cgctgccgtc gcggacttcg tggccgagaa ggatcgcgag cactcccgcc
     3961 tcgccggatc cgacgtgacc cacagcgagc gcgtcgtgga cgggcgcagg ggctacgtct
     4021 ggaaccaccg cagcccctac ggctactggt actacgcggc gtggttcccg cagcgggtcc
     4081 acaccgtgcg cgtcgagtgc atcgccaagc agcaggaggc tcgcttcaag cgcctctgcg
     4141 ccgaggcggt cggctcgctc gagttccgct aggtcctcac gcgcggctgc tcgaagcgcg
     4201 tgctgaacgg cgtggcgccg tcgtcgcgca cgagcgtcag gtcgaggtgc gccgtggccc
     4261 cgtccctgcg cacctcgagc agcgcgaagt gcccgcgctc gtcgtcggcg taggcgaccc
     4321 cggggacccc gcgcgcgtcc tccgccacgt tcggctgcgt gagggtgatg tcgttcggcg
     4381 tcgggatgcc gagcgggcac ggccgtgcct cgaacaggcc gtcgcgctcg tagaccatcg
     4441 tccagtgggt gtcgccggtg acgaacagcg tccggcccgg cacgcgctcg gcgatgtgcg
     4501 cgcggatcag gtcgcgctcg gccgtgtagc ccgccgccca gctcccgtcg cgctcgttcg
     4561 ccttgagcgg agcgagggtg cacggcgagc agaccacctt gaacggagcg cgcgacgcgg
     4621 tcagcgtcct caagagccaa tcgcgctgag cggccccgag gagcgtcttg tccggcgtgt
     4681 cgggggccgc cggatcggtc ttgtgcaggc gctggtcgag gatccagaag tcggcgagcc
     4741 cggcggagaa gcggtggtgc acgcggggct ccatcagcgc ctcccacggc gccagccccc
     4801 agggcacgag gttggtcgag ttcgcgtcct gcaggccgta gtcgtggtcg tcgcgctgcg
     4861 tcgcgaagag cgaccgctcg aggatcggcg cgagcctcgg gttcgcgagc agctcccgcc
     4921 acatgccacc gtaaccggag accgtctgcg cgagcggccc cacggtgtcg ggatagttga
     4981 ggtcgccctg ccagacgaac aggtcgggct cgcggcgggc gagctcgtcg aagatcgggc
     5041 cgaactgcga ggcgcacgag ccgatggcga tcgtcgcgtg cttgccgctg cccgcgcggg
     5101 ggagcgcgcg gaagctgcgc gccgggccga cggtctcgag gccccggcgg cgcaggcggg
     5161 cgcgccagta cacgcgccgg ccctgcctca ggccggtcgc ctttgcgaac gcggcgtcga
     5221 acccgttggt acgggccggg cggatgcggc ggctgcgcct gaaggccgag ttgtcggcga
     5281 tctcgatctc cacgcgggcg ggcacgtcgg cggcgacgcg cagggtcgcg ccgcccgtca
     5341 ccggcgtgcc gccgctcgcc gcgaccacgg aggccggtgt cggcgcgggg cgctcggcgg
     5401 cgcggaggtc gatgcgcgtg aaggccgcgg tcgagcgctc gcgtatggcc tccatcactc
     5461 ccgcgcccgc cggagagccg atcaccgcct ggccctcctg aaccccgtac gggagcaggt
     5521 gcaggttgcc gagcgccgcc gcggggccgg ggccctcggc ggggaagagg gtgcgggcgg
     5581 tcgcgagcac gcccgggtcg ccggcgcgct ggagggcggc cgcggagtcc gcggcggcga
     5641 cggtgaccgg cgctccgccc gagtcgaggg ccgcggccag cgtggtcggg ttcgcgccgc
     5701 tcgcggtgag ggcgagccgg aacggtgtgc tggggcgcgc gagcgctgtg cgtgcgagct
     5761 cgctgaggcc ggccggcgag cggcgcagga gcagcagcgc ggcctgctcg tcgtcgtaga
     5821 ccgcggcgta gtagtcgcgc ggtcccgtgc gccgcagcac cagtccggcg cccgccccgg
     5881 ccgcgtcgac cgacgcgctg atctcaccgt cgcgaaagcg ccggtcgacg gcgaaggcga
     5941 ccggccgcgg gtcgttgggg aacacgtcgg aaccggcctc cagcacgccc acgccgttcg
     6001 agcggcgcag gttggcgacg cccgcacagc gccacttcgg ccaaccctca cccgccccgg
     6061 ccgagccgaa gtcgatcccg ccggtccagc cggccgccgc cgcgccggcg gggtcgcgga
     6121 gggcgagcgc ggcggccgcc gcggcggcgg cggccagcag ctcgcggcga ctgacgcgcg
     6181 ggtcctccac cggtcagctt tcccccatct cgcccagcat gcccggcggc gcgccgcgcg
     6241 gcgccaggtg cggcaggacc tccgccgcgg cctcctccag taccgcctcg tcgcccgcgt
     6301 accagctgct cggccgcggc cagtgcgtga tcacgtcggt gaagccgagc tcggccgccc
     6361 gcccgacctg ctcctcgaag aacgctcggc tcgacagcga gaagcgcggc gcggagtcga
     6421 gcatcaggta gcggtcgccc ggcccctcga agcgagccgc acggtcggcg accgaacgcc
     6481 accaggactc gaggtcgtcg gcgcttccgc ccatcgtgat ccaaccctcg ccgaagcggg
     6541 cggcgagtcc catcgaccgg cgcccgttcg ccgcgaccac gaacggcacg cgcggcgact
     6601 gcacgcagcc cggggtcgtg cgggcgtcga cagcctcgta gtacgagccg cgccaggtca
     6661 cgcgctgctc gcgcagcagg aggtcgagca gctcgacgaa ctccgcgaag cggtccacgc
     6721 gctcgcccgg cgacagctcc ggctggccga gcacgagcgc gtcgaagctc ggtgccgccg
     6781 cgcccgcgcc gaggccgagc acgaggcgcc cgccgctgat gtcgtccagc gcggtgatct
     6841 cgcgcgcgaa gtgcacgggg tggcggaagt tcggcgacgc gacgagcgtc ccgagccgga
     6901 tcgactctgt cgcgccggcc gccgccgtga gcgtcacgat cgagtcgaac cacgggccct
     6961 cgacgaggtc gcgccagccc agatggtcgt aggtccacgc gtgcgcgaac ccgtactcct
     7021 cggcgcgggt ccagcgtgcg cgcgcatcgg cccagcgctg ctccggcagg atcacgatgc
     7081 cggcgcgcac gtggcgatcg tagccctgtc cagccgcgcc ggcgagggag cgcctacgcg
     7141 agctcgggcg gcaggaccct ggggccctcg gggtaaatcc agtcgagctg ctcggcggtc
     7201 atcgtctggg tatccaaggc ctcgggcggc gcgccgacga cgagccagag cgcgtcggcg
     7261 tcggtgtcgt tgaagacctg gcgcacggtg gccggctcca cgagcagcgc cgagtgtgga
     7321 gccagcgtca gggtctcgtc gcctatgcga acgcggccgg tgccctcgag cacgacgtag
     7381 agctcgtgct ggtcggggtg gcggtggcgc gtgctggcct gccccggggc cagccgccac
     7441 atccgcgcgc cgaacgtcga ggctccgagc tgtccggcga ggtccgtgtt cagcacgccc
     7501 atcaggttcg acggacgcca gaaggcatcg gcggcgttca ggaccgtata gctcacgcgt
     7561 gaagcgtatg gaggaggtgc ccggacttac tggcggtatg cgccacaaaa gccggacacc
     7621 tcccgggcgc cggcgaccga cccggcccgc gcttgacaat gggaagatat cttcctattg
     7681 tcgccgccgt gccgcggcgc aagcctggaa ccctgctgcc cctcgagatc gagatcctcg
     7741 aggccgcgct gtcgatgccc gcgtcgggcc agcggggatt ccacggattc ggtctcgctc
     7801 agaccatgcg ggagcagagc ggggcgcagt cgctcaccgc gcacggaacc ctgtacaagg
     7861 ccctgagccg gctcgaggag ttcggcctgc tcacctcccg gtgggaggac gcggcgtcgg
     7921 cggaggggcg gccgcgccgg cgcctgtacg agctcaccgg cgctggtgcc caggcggccg
     7981 agcgggcccg tgcgggcagg gcgccggcca aggccgtcgg acgctcgccg cgcgtggcat
     8041 cgaggtccgc gtgaggccgg agcgcatggc ccggctggtg gcccggtggg tgcggatcta
     8101 cacgcgcgac ctgcccgatc cgctcggccg gcgccgggcc ggcgagatcg acgccgacct
     8161 ccacgaccac atcgcacacg aacgggctca cgggacgagc gacggtcgca tcgccctcag
     8221 cgttctgtcg cgaatgctcc gtggcctgcc cgccgacgcc tcgtggcacc gccgccacgc
     8281 ggaggcgcgc aggaccgtgc tgcgatccgc cgtccgcgtc gcgctcgcga cctcgctcgt
     8341 cctgctggtc ccgctgttcg cgatgctggt caccgacgcg gtcgcctgga gtccgttcga
     8401 cttcgccgcc gccggcttcc tcctgttcgg tgccggtctc gcgtatgagc tggtggtgag
     8461 gaggcccggc gccatcgcct acagagccgg cgtcggactc gcgctcgcgg cggcgttcgt
     8521 cctcgtctgg ctggtcgggg ccgtcggcgt gatcggggaa gacggcgacc gcgccgacct
     8581 gatgtacggc ggcgtgctcg cggtcgggat cgtcggcgcc gccgtcgcgc gcttgcggcc
     8641 acgcggaatg gcacgcgcgc tgctcgcgat ggcggtcgct caggcgctgg tcgccgcgat
     8701 cgcgctgctc gccggaaagc accaggacac ggtcagctcg ctccccgagc tcctcgggct
     8761 gaacgggttc ttcgtagcgc tcttcgccgc gtcggcctgg ctcttccgcc gcgccgcggg
     8821 cgggcagccg cccgcctgac ccgacacccg gctcgctcag agcccgagaa ggagtcgcat
     8881 ggcgtcatcg acggcgcgca tctcactcgc atcgaggcga ccgtccgacc ccccgaggcg
     8941 ctgcgggtcg acgacggtgg tctgctcgac gaggacacgg gtctcctgac cttggatctc
     9001 gatcgtcggg cggaagctcg caggccgcgc ttgggtcgag gtcggcgcga ccaagaccgt
     9061 gctcagtccc atgaactcg
//
LOCUS       DASVPL010000100        34970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_169098, whole genome shotgun sequence.
ACCESSION   DASVPL010000100 DASVPL010000000
VERSION     DASVPL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 34970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34970
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169098"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VF517_04615"
     CDS             <1..548
                     /locus_tag="VF517_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052253.1"
                     /translation="SGGAALPGGSGMAGGSGASTPGGSGGSGGGGTRYFTYTVDVKFG
                     SRKPKSYKSLATLDLLPDDQNPIVSFMGMTDNAKTAVFFVVDPAFEADGEGKCSPSAD
                     DCRFIYLGVEDDRDEETLSAAEGALEYTLQLDRINIKRLSENEAVGNAQPESDTPDTP
                     TAAKRRQSRKTLMTVPLAARR"
     gene            complement(632..874)
                     /locus_tag="VF517_04620"
     CDS             complement(632..874)
                     /locus_tag="VF517_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052254.1"
                     /translation="MIIGFALFLLCGIVFGYAAPSGWALLPVVLPIIVGLYTGLMDVF
                     DTELIVLMVVGVAVTLVGILVGRAILFSTEGRRSTA"
     gene            911..2077
                     /gene="aroC"
                     /locus_tag="VF517_04625"
     CDS             911..2077
                     /gene="aroC"
                     /locus_tag="VF517_04625"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX8052255.1"
                     /translation="MVAFRFTTAGESHGPGLVAIVEGLPAGLELAPADLDADLARRQL
                     GHGRGGRMKIETDRAEVTAGVRHGKTLGSPIAIRVANRDYANWEERMNPWPVDGQPDE
                     VHLPRPGHADLAGVQKYGLTDVRDVLERASARETAARVAAGALAKAFLRRLGVEVRSH
                     VIAIGSVTAPAPDDLTAADFEGVDESPVRCLDADSTAAMIAYIDQARKANESLGGIFE
                     VRAFGLVPGLGSHISWETRLDGLVAHAIMSIQAMKGVSVGDAFDVATRPGSQAHDEIF
                     FDDEHGFHRETNRAGGLEGGMTTGDPLVVRGAMKPLPTLTKPLRSVDIATKEPAQALR
                     ERTDSVTVPAAAVVGEAMIAIVLARAYREKFGGDHIDDVAGALDAYRSRIGWRR"
     gene            2065..3630
                     /locus_tag="VF517_04630"
     CDS             2065..3630
                     /locus_tag="VF517_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase"
                     /protein_id="HEX8052256.1"
                     /translation="MEAVAAGGSAAGAAAGAGRRALVFVGFMGAGKSSAARAVAAELG
                     VRPVDSDRELEQSLGQPIETFFDRHGEAEFRRREEELIVGLLARADGGVVALGGGALG
                     SDRVREALAPHVVVHLEVSSDAAWRRASGKGRPLARDRARFDQLFAERRARYEASADA
                     IVLASDRGVAARALPSLLALRDGPPGTRCVWASGGAAEYPVFLGRGLLASGFFPPLGG
                     RRFAVSDENVAPLHRVEADWSWEIPPGESEKTLGRAEAVLRGMASAGVARDDLVGAVG
                     GGVVGDLAGFCAAVYQRGIRHVQAPTTLVAQVDSAYGGKTGVDLPEGKNYAGAYHQPS
                     AVMVDPAALDTLPAAERAAGYAEVVKTALIAGGELWSRVRGGSEPDAAIVLGCLRTKL
                     AVVAEDERDEGRRQVLNLGHTIGHAIEAATGYERFRHGEAVAIGLLCALRLSGREQLR
                     SEVAELFRARGLPLSCPGVDADAVVELVARDKKRRGGSVPFVLVESPGQVTPGHELAE
                     DAVRAAVEEVCAE"
     gene            3627..4073
                     /locus_tag="VF517_04635"
     CDS             3627..4073
                     /locus_tag="VF517_04635"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX8052257.1"
                     /translation="MKNRVEVMHGVNLDQLGVRDPAVYGSQTLTELEVRVKRFAQELG
                     LETTFSQTNHEGEFCEGLHEARGTVDGLILNPGAWTHYSYAIRDALEVTALPAVEVHI
                     SNIEGREDWRRASVIRDLCIGYVHGRGVDGYRDALEMLRAELGGGS"
     gene            4070..5125
                     /locus_tag="VF517_04640"
     CDS             4070..5125
                     /locus_tag="VF517_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX8052258.1"
                     /translation="MSRADRVEALLADAELDALLVTNLENVRYLTGYTGTNGVCVIGP
                     DLRAFFTDFRYVAQAERQVHGFDRALGKQDLLGDAAGRLSGRVGIEDYDLSVRRHERL
                     RGLVGEGVELVPAGRCVERVRAVKEPEELDRVRAAAALADELFRGVVERGLIGRSERE
                     IAFELETEMRRRGAAPSFPAIIAGGPNGALPHAEPGEDPIARDTLVVFDWGVELDGYC
                     SDCTRTLATGSIPDDLAEVYELVRATQVATLDEVRPGAAGAAVDGFARERIAAGGHGE
                     RFGHGLGHGVGIVVHEGPTLSKASEDTLVAGNVVTVEPGVYVPDAFGVRIEDLVVVAD
                     DGPEILSGFPKGLTEVG"
     gene            complement(5147..5605)
                     /locus_tag="VF517_04645"
     CDS             complement(5147..5605)
                     /locus_tag="VF517_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX8052259.1"
                     /translation="MFSSLALRLRSRRGFTLIELVVTVLLLTILAAIALPAYLEHQKK
                     GKDSDAESNARNLVSRVELCYATEENYTNCDTNEELGADTNLPYGTAPGQVSVVSATT
                     STFKVTAISKAQSDGSNHTYTIDHGVSGVNDRTCTAGSTNKKGGCRSGVW"
     gene            5717..7135
                     /locus_tag="VF517_04650"
     CDS             5717..7135
                     /locus_tag="VF517_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052260.1"
                     /translation="MTVCVLYPRFALLAAISERDELLAAPVALAPAPGGTQVVGQVSA
                     AAEAFGIAAGMRVGEALARCPELRLVPPDPECERAAWGRVLDRLESIGAASESDVPGI
                     AFFEDEGLRRLHGGQVDDVLTATRRTLGGAVRLGCAPSRFASYAAALQARPRRRGGRG
                     RAPGGGSVPRAAGGGTGIATRGAGGAVFVPPGAVRAFLAPLPVALLRTRPELAALPDT
                     LERLGVRTLGEFAALPPAAVSERFGHPGLLALDLAHGRDTALDPRRPPEPVVERLDLP
                     EAALGPQLERALELLVARVLARRERRGRTVRALALVARFVEGGTWRVRVTLRRPSADP
                     DRILTAVGVKLPELPAPAESLGLEVEAFGPPAHDQGRLLPDTPAELRRRRIAEAVGQA
                     RQAAGPDAAMRVLEVDPGSRLPERRAVLAPFPVEENGDGPAPRGRPASRGGSSPRGGS
                     APRGSARGGPAPRGGSAPGGKR"
     gene            7132..7374
                     /locus_tag="VF517_04655"
     CDS             7132..7374
                     /locus_tag="VF517_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052261.1"
                     /translation="MSVRRLAHPRGVCVRVDDAGAPAALQGEPVDSVLEEWVVEDRWW
                     TDRPVRRRYFELVLAGGRNAVVFKELGGRGWFEQRG"
     gene            7638..10922
                     /locus_tag="VF517_04660"
     CDS             7638..10922
                     /locus_tag="VF517_04660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX8052262.1"
                     /translation="MPYVELHTHSAFSFLDGASHPVELAAAAAEQGHSAIALTDHDGL
                     YGSMELAIAAKPLGLRAIVGAELTLDDGHHLTLLCEDRTGYANLCRLVTNAHAGTRER
                     PADPVPPSTSLEDVERFAEGLVCLSGCARDGAVAARIERDEHAEAAATAKRLRRAFGP
                     DRFRIELQRPFDRHDRRRNRLLAQLAERIGIPCVATGNVHAHSRERATLQDAFVAVRT
                     GSTLDESEPIRRGNSSHVLAPPNQMAARFADHPDAVEESGRLAERLTFDLTTDLGYRY
                     PGAEGGSADHDLAELCRSLLGSRYEGRASRAAAEQRLEKELAIVRSLGLSGFFLLHRD
                     LLELAREVAAEVRGPDSARALLPPGRGRGSSVSSIVCYLTGLSHVDPVENDLFLGRFL
                     NEDLSALPDIDLDFPRDIRETLIPRVHERYGHDRSALVCAFATYQTRSAIRDFGKALG
                     LPAGEIERAARAAEPWGGGDVEPHLVDAIGRRRAASPRWQALVRLTRDAWGLPRHVTQ
                     HVGGMVISTQPLNEICPIQPAAMSGRQMVQWDKDSCGDAGFLKIDLLGLGMLSAVERC
                     VDQIARAREERIDLSRIPFDDPAVYREIQAAETMGVFQIESRAQMQMLKRTRPENLDD
                     LTVQVALVRPGPIQGGAVHPYIERRKALRADPGYRVPYDHPSLEPVLRDTLGAIVFQD
                     QVIEVAMAFAGFTPGEAEGLRRAMSRNRSVAAMRSYEVKFVEGAMERGASLDTARKVY
                     AQIVGFSGFGFPKAHSAAFGLLAYQSTWLRVHYGPEFLCALLNEQPMGFYPPDSLVHE
                     AQRRRIPVLPPCVLRSEAECSVERVEGGALAVRIGLGYVAGAGKEDVAEVVAERERSG
                     PFRGIAELAGRCGAQRDALERLAWAGACDALVEDGRRQALWLLGVSAPGVTVAGGTQL
                     ALPVEPGAAPELRELSPWERLIADYGSTRITLHEHPMELMRPSFDAGLVTSSALAALG
                     HGARVRTAGLVVARQRPATAKGVTFMLLEDEHGAINVIVAPPVYEAHRLAVRAEPLVY
                     VEGRLEHREGVTNVVATAVHRLERPDIPLGEVRHIEPRRAWSTEEAAGDLRAVAPTAH
                     SFGRRG"
     gene            11051..12379
                     /locus_tag="VF517_04665"
     CDS             11051..12379
                     /locus_tag="VF517_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8052263.1"
                     /translation="MRNRGLLLLALAIVAALSIPSTSSAATGTVIPGHYIVVLEDSAD
                     VDAMVAEHVDLGAVVGHTYRSALHGYAARLSPTIRALVKADPRVARVVPDREGVATQA
                     QTLPTGVNRIDADLGSQAAGDGTGTTPGAVAIMDTGIDTTHPDLDVAGGINCLGSYST
                     HDGTIGDENGHGTHVAGIVGAKDDGEGTVGVAPGVRLWSVRVLSSTASGTTSTQLCGI
                     DWVTANAQALGIKVVNSSMVLFGAVDDGNCGNTSGDPLQQAICTSTAAGILWVFAAGN
                     SNASFAGSAGPSFDQVLTVTGLADGNGVPNVPTTTTFTCPAVNGTRKYPNYAPETDDK
                     TYSYSNYAVSGDAAHTIAAPGSCIWSTYKGGTYGNMTGTSMAAPHVTAAAHLCILSGQ
                     CPGTPAETIQKLRADAEAFSLGDAGFGFTGDPLRPVTGRYYGYLVRAGLY"
     gene            12395..13318
                     /locus_tag="VF517_04670"
     CDS             12395..13318
                     /locus_tag="VF517_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8052264.1"
                     /translation="MAGRIVVGTSSWADPGFVEEWYPPGMPARERLPWYAERFECVEL
                     NSSFYAVPDPGLVERWVRATPDGFVFDVKLHRLLSRHSADLKSLPPDLREEAQTNERG
                     RVRLTPELEMAVADRVLEAVAPLERAGKLGAFLLQLTPAFSPDKHRLDELDGLLERLS
                     PRVVAVELRNRGWVSDKRAPETFAYLSERGAAFVSVDAPREEHVPIMPPVDAVTNEAL
                     SYIRMHGRDAHGYMHGKTVAERFAWDYSDEELEEIAGRVRGLAEQAHEVHALFNNNRG
                     ADAPSAASRFRALIGQDAGPPPDVGQQLRVL"
     gene            13315..14502
                     /locus_tag="VF517_04675"
     CDS             13315..14502
                     /locus_tag="VF517_04675"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX8052265.1"
                     /translation="MSAPVRIATGLATDDAGPDAFAEAAARAQLGLGGAQADLVVAFA
                     AAPNLDHAEEGLAAVEERLGPYALIGCGAQGVIAGGRELETGGVAVWAASLPGATLRP
                     FHLDAFDAGDEGLAIAGLPGLTGVEAVLLLADPYTFPVEPLLDRLAADHPALPVIGGL
                     ASAGAVPGRATLLHGGGVEERGAVGVALAGVDVRPCVSQGARPIGPEMVITACDENEI
                     HELASRPALARLHEAIGELDPEERALAGRGLLLGIVIDENQPDYERGDFLIRGLLGID
                     EENGSVRVGERVRVGQTVRLQVRDGESADEDLREALARHADALSGPPAGALLFSCNGR
                     GSHMFGEPDHDALALDDAFSGAPAAGFFCAGEIGPVGPRSFVHGFTATLAVFAGSGPG
                     SAI"
     gene            complement(14499..15932)
                     /locus_tag="VF517_04680"
     CDS             complement(14499..15932)
                     /locus_tag="VF517_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8052266.1"
                     /translation="MTEPIQRPALEQAAAVRAGEVSARELVEASLAAIERLDGDVNAF
                     VTLVPERALAEADAIGPGDARPLAGVPMAIKDLGPVTEGIRTTFGTSLSGDWIPPFDS
                     ALVTKLRAAGAVIVGKTNAPELGITPVTEPARFGPTCNPWDLTRTAGGSSGGSAAAVA
                     AGMVSLAHGNDGGGSIRIPASCCGLVGLKPSRGRVSSAPLVDSPSALVAEGVLTRTVR
                     DTAAALDVLAGYEWGDPVTPAPPRSTFLEAAGHAPARLRVGVTTVPPVDAEVHADCVA
                     AVTATAALLAELGHDVEESAPKWEAREHLDAFLAMWATDASLGAERIAPLGGAPLDPS
                     ATEPLTQALIENARGRTARDLLEGLTTLRAYTRRVTRWWADHDVLLTPALAQPPVPHG
                     ALAAAPGEPGLEAFWKGSRFVPFSAAANVTGQPAIALPLHVSASGLPIGVQLMGPPAG
                     EELLLALAAQIEAARPWADRRPALALA"
     gene            15966..16697
                     /locus_tag="VF517_04685"
     CDS             15966..16697
                     /locus_tag="VF517_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8052267.1"
                     /translation="MSSADRAPDGVVRVRAPNPSPLTLDGTNSYVVGRWVVDPGPDHA
                     GHLDALLDAAGGRLDGIVLTHEHIDHAEGAQPLAERAGGVPVVQPREGDEVGPFRAIA
                     TPGHAEDHVCLLMGSVCFSGDTVLGEGSVFVGFGGASMGDYMASLERLRELELEVMCP
                     GHGPFVWDPRARVEEYIAHRLDRERRVLEALAAGARTHDELLDLAWDDVDFTDAPMLR
                     LAAAATLEAHLEKLRGEGRAPQSGA"
     gene            complement(16900..17358)
                     /locus_tag="VF517_04690"
     CDS             complement(16900..17358)
                     /locus_tag="VF517_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052268.1"
                     /translation="MSTESPSTESPSTSSAGVEDAAGTVASVGVVGAAGTVASAGVIG
                     SAGTVASAGVIGSAGTVASAGVVGGAASVASAGVLFSAGTVASAGVVAGLGNVNCTGC
                     VGCVGCVDCEGCVGCIGCVGLRGAVGVSGNRKRRRRRRRSLRTEFRALLP"
     gene            complement(17355..18386)
                     /locus_tag="VF517_04695"
     CDS             complement(17355..18386)
                     /locus_tag="VF517_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX8052269.1"
                     /translation="MASSAPGRSLIHERFPPTAATLPRIPLCSLPTPVRAAPGLGADV
                     WVKDDARSAEPWGGNKPRKLEWILADARARGRRTILTFGGIGTNHGLATAVYARRAGI
                     DCVLALAEQPRDEHVERQLGRLRAAASRVYLTGTARRTALAAPLIALRHSQRRPPRAP
                     YLLAPGGSSPTGALGFVEAALELAEQVASGELPEPRSVVVALGSGGSAAGLAAGFALS
                     GLGTRVHAVLVNDQLRLSEASVLRLAGRTLRLLRRRGADVSGARLDPGSVRVVEGFMG
                     AGYGHATAAGDAARALGADAEGLALDPVYTGKALAALLAGAAGGGPVVFWNTNNGLPY
                     GDGPVGSTA"
     gene            complement(18376..18615)
                     /locus_tag="VF517_04700"
     CDS             complement(18376..18615)
                     /locus_tag="VF517_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052270.1"
                     /translation="MGDAPSEAQIMQRLERLERQVRMLAERTGVGIEDPAAEVDPEVV
                     ALVREGKRMNAAKLYAERTGSDFLTAQRVVNGLGL"
     gene            complement(18628..18894)
                     /locus_tag="VF517_04705"
     CDS             complement(18628..18894)
                     /locus_tag="VF517_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052271.1"
                     /translation="MDEVWAGTTLYAFDGRVLEVFGFPGSESMRFHVLNLRIEVDEPD
                     RKGRRWLRVRPLHGSGGMMVEVQAEEWPALGPFLDRVGTAIAAQ"
     gene            18926..19363
                     /locus_tag="VF517_04710"
     CDS             18926..19363
                     /locus_tag="VF517_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052272.1"
                     /translation="MESDFDRATRAASANEMPPPFRAALDEHAALVGIEGPVLSTARA
                     AAVTESTPHRRRLLRRQRPHVTWMVVCADVLAVVGDASGAPVGSLYRLAELEAKPFSS
                     PLVQDEGLEATALPVGGSERVSVFLPLAPGPPRDAIAAALALA"
     gene            complement(19581..20144)
                     /locus_tag="VF517_04715"
     CDS             complement(19581..20144)
                     /locus_tag="VF517_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052273.1"
                     /translation="MVWHLREEQDFMKSLPSPALAIAGIALFCALGGTGYAATKLAKN
                     SVTSSHVKNESLTGADVRDGSLTTDDIRNGGAVGEVRRVDGPKSSEADGVTSSRAECP
                     EGFNVIGGGHNVGTFDSPVSATPYSSFYSVISVNHYEGSNPQFMIAEAQAFAVCARTS
                     GSDFRRRKAKELSDFRGRLARAEDAAR"
     gene            20367..20927
                     /gene="efp"
                     /locus_tag="VF517_04720"
     CDS             20367..20927
                     /gene="efp"
                     /locus_tag="VF517_04720"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX8052274.1"
                     /translation="MISTNQFKNGTHIDVEGTIFKIVEFQHVKPGKGGAFVRTKLKRT
                     TDGNVIDRTFRAGEKFRPVRTETRKMQYLYSDGTDAHFMDVESYEQFTVPEEAVREPL
                     QWVLPSQEVEILYVDDEPSDVQVPAAVELAVAETEPGVKGDTASGGGDKPATLESGVI
                     VRVPLFVNVGDRVRVDTRSGAYVSRA"
     gene            20938..21315
                     /gene="nusB"
                     /locus_tag="VF517_04725"
     CDS             20938..21315
                     /gene="nusB"
                     /locus_tag="VF517_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX8052275.1"
                     /translation="MRRSDQRRAAVVALYQRDVTGRSLEETLGRTATIFTRELATGTD
                     SHIEELDGLIERYAEDWTIDRIAPLELNIMRVALHEMLHREDVPDEVALDEAVEAAKE
                     LCGAGAPAFLNGILGAVQRERVT"
     gene            21312..21503
                     /locus_tag="VF517_04730"
     CDS             21312..21503
                     /locus_tag="VF517_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052276.1"
                     /translation="MSGLDDAVAELERAAARLREPDLPPEEAAELVERCAEIAARVGA
                     ELDRRADPDRAAPGQETLL"
     gene            21539..22381
                     /locus_tag="VF517_04735"
     CDS             21539..22381
                     /locus_tag="VF517_04735"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX8052277.1"
                     /translation="MQARVDEYLAGLTFAREGGVAGLEEAMRYSLLAGGKRIRPVLAM
                     ATAEALDRDPADVLPLAAAIELIHTYSLIHDDLPAMDDDSLRRGQPTCHVKFGEDVAI
                     LAGDGLFAEAISLVATEQQGEPGHVLAALGHMVAAAGVGGMVGGQYLDIKAGDQADLR
                     RVHELKTGRLIAASIECVLMLGGLAGHATIPWSRFAVELGVLFQIVDDILDVTGDEAA
                     LGKPQGSDERQGKRTYVSVFGLDRARELAAQAHAKARAALAEADGRTGTLERTTDYIL
                     TRRT"
     gene            22378..24315
                     /gene="dxs"
                     /locus_tag="VF517_04740"
     CDS             22378..24315
                     /gene="dxs"
                     /locus_tag="VF517_04740"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX8052278.1"
                     /translation="MSLLDKINGPADLHQLSEPELQDVAQEVRELIIDTIGEIGGHFG
                     ANLGTCELAVALHSLLDSPRDKVLWDVGHQAYPHKILTGRRDRLGTIRKYGGLAPFCS
                     IPESEHDIMGAGHASTSVSYAVGLKEAMRRGHEEDGKVVAVIGDGALTGGVAFEALHN
                     AGGMDVPIVIVLNDNGMSISPNVGSLSRYFTRIRLNPDLHRAREVVESGLMKMPAGLG
                     ERIERLGPELKASIKAFWAPGLFFEELDVAYVGVVDGHDVTALRKSIGEALEAERPVV
                     VHIKTVKGKGFAPAEEGGLEGMEKWHAAKPASIVGRAAAPKKPDDAAAPPKPPQYTTV
                     FGNSLVEECRRDARVCGITAAMNSGTGLNILQKELPERYYDVGIAEQHAVLFAAGLAL
                     GGMKPVAAIYSTFLQRAYDQIVHDVCLQNLNVVFAMDRAGLVGDDGPTHHGAFDIAYM
                     RCLPNLVMMAPRDEAMLADMLHTALRYDDGPVALRYPRGEAEGVPLPSRPQPIEIGRG
                     EVLREGSRVALIGYGFGVSLALGAADLLEQHDVEPTVVDARFAKPLDGELLTQLATEH
                     ELLVTVEDGVLQGGFGSGVGELLLDRNLAADTRLVRVGLPDRFVTHGKPALLHKEVGL
                     TPEAIVERVLADSRERGGALV"
     gene            24501..25283
                     /locus_tag="VF517_04745"
     CDS             24501..25283
                     /locus_tag="VF517_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX8052279.1"
                     /translation="MVKTRLDALLVDRGLFESRSRAAAAVIAGSVRIGSDGRRAAKPG
                     QMVGDDMPVAVDAPPPYVSRGGVKLANALDALGVEVAGRRCLDVGASTGGFTDCLLQR
                     GAEHVVALDVAYGELHWRIRNDERVTVVERSNARALEPGALPFPPDLVVVDVSFISLG
                     KVLPAVLGCAAERFDCLAMVKPQFEVGRERLGKGGVVRSPEHRREAVATVARMAAEDG
                     WSVLGFAPSGLPGPAGNRETFVWLGEPGRDGGAPARPDGVDV"
     gene            25280..26140
                     /locus_tag="VF517_04750"
     CDS             25280..26140
                     /locus_tag="VF517_04750"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX8052280.1"
                     /translation="MSGAPRRITLFSHGRIDDTGPAVQRLVDAAAAAGVEVLIPHGEA
                     EKHDISEREGCRLGVDPSEDTDVAVVFGGDGTILNALRSCASSEAPVFAFNYGAIGFL
                     STVEREDLDDGIARLLEGRYEVLGMPALAVELDGQRRVGVNDISLHRRPNSRVALLGY
                     SVRGDQLGQVRCDGLVAATPVGSTGYNLANGGPILAWGVEGFVVSFIAPHTLTARALV
                     VAPDDVLTVHNLSDTDPVDVATDNRSVAELAPGAEIEVHFVQDQVRLAQVEGANFYHR
                     IRAKFGRLAY"
     gene            26311..27228
                     /locus_tag="VF517_04755"
     CDS             26311..27228
                     /locus_tag="VF517_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052281.1"
                     /translation="MSWIRVPLIWERVEREGFGQVDALVDAARGRGFQVQLNLIPPAP
                     ASATGDGRPGVFRPDPAAFGAFARRAAEHYRSRVRRWSIWNEPNLVNWLRPVAEMPEM
                     YRRLYEAGYAAVKGVSADAQVLIGETAPYVRRGDGMAPLWFLRRMVAGGPLVADGYAH
                     HSYDFEAPPERDYPGDDNVTLGSIERLIGALDRLAASGALRDGHGRALDVWITEFGYF
                     QTTERALPPARRADYVRRAFDLAASHYPRVRQLLQYLLVSPPEGFPGGRFDTSLLTHA
                     GRSTPAYGALAAWSRAAVRARRALPPPGD"
     gene            27308..27859
                     /locus_tag="VF517_04760"
     CDS             27308..27859
                     /locus_tag="VF517_04760"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8052282.1"
                     /translation="MTQLALLAQEAGVHERTLRRAVNQGTLRAHRRSPRTLELPLSER
                     LYVRRSWPLIAALRSALRTDPNVRFALLFGSTARGTDTPASDVDVLVELLDPGLERVI
                     DLGERLTAASSRPVEIVRLEAVRDDASFLGDVLAEGRVLVDRDEVWPSVRSRERMLRG
                     RRRQEEVRRVEAALAGVDQLLAG"
     gene            27863..28378
                     /locus_tag="VF517_04765"
     CDS             27863..28378
                     /locus_tag="VF517_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052283.1"
                     /translation="MASAPRRADRGRWRRDILRHLEDFPRQYAALETAMAAFGGDFDL
                     KLFKQAFETHEDLEAYNRVQSVERALGRVQNFVAELADAGVTLARLPRPPMTKDGCQA
                     QQAFEALRDAKVMGGSLCRQLTRAQRARSRIEHGYVETPAGDVHRAAELVHESALGFI
                     AAYRPWIAEYL"
     gene            28431..30167
                     /gene="recN"
                     /locus_tag="VF517_04770"
     CDS             28431..30167
                     /gene="recN"
                     /locus_tag="VF517_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX8052284.1"
                     /translation="MLLELRVENLLLIDRAELRFGPGLNAITGETGAGKTVLAHALDL
                     LLGGKARGGIVRPGASEAYVEGVFELRPGLLDGDELADLRERVPDDADEIVLGRRVGA
                     EGRTRAFVQGRSATAADLRELGGRLLSFYGQHEHRRLTVGSAQLEILDAFCGEAHLAL
                     RERYAERHRRAGDLRRELDELRARTGARDRDRDLLAFELAEIDELGPDPGEEEGLMAE
                     RGRLREVDGLRMAAGAGAEALAPEDPEAPGAEAALADAERLADGVAGADAELDALAER
                     LRALRIEAEELGGDLRRYADGLEADPARLEEVEARLDSYDRLKRKHGGSVEAVLEHAD
                     RCRTELARLDSAEEDSERARAELAAAESEERALAAKLTKARTAAAPKLAKAVLAELRQ
                     LAMEDASFEVVVEPRGAASRGAAASPGGAAPPAADLGPTGADRVELRVAPNPGVAPGP
                     VREIASGGELSRVMLALMSVANSGGDATLVFDEVDAGVGGQTARAVGERLRGLAAGRQ
                     VLCITHLPQIASMADSHFRIEKDTGGDVARTTVEPLEGPEVVAELCRMLGADTSDAGA
                     RRHAEELLATAA"
     gene            30256..31461
                     /gene="steA"
                     /locus_tag="VF517_04775"
     CDS             30256..31461
                     /gene="steA"
                     /locus_tag="VF517_04775"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEX8052285.1"
                     /translation="MAAPRIRPRSLRPRPPAALANGRPEPATGFTGPARLGTRTKRLV
                     KRIGPEDIAIIDHAGIDRVSGEDLVACGVRHVINVATSAAPHYANRGPSILVDGGVHL
                     VDMPGAPLFEVLKDGQTVTLDGGKLLRDGEVVGEGQVLDRETVLRAYELAERRIGEAL
                     EAFAENTMTHIREERELLSGEIALPDFDTTFRDRPALVVVRGVDHKRDLRILRPYVRE
                     TNPIIVAVDGGANAILEEGLQPDMIVGDMDSATDDALRSGAELVVHAYPDGRAPGRKR
                     LDPMGLEYKVVPAPATSEDVALLIASEKGANLIVSVGSHFNLVEFLDKNRAGMSSTFL
                     TRLRVGEILVDAKGVSRLYRPSAGGRPILLVAAAALAVLIVIVATSETLGPLLDLLWL
                     KLQVLLGLE"
     gene            31468..32310
                     /locus_tag="VF517_04780"
     CDS             31468..32310
                     /locus_tag="VF517_04780"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX8052286.1"
                     /translation="MLDFRYHALSLVAVFLALGIGILLGTTIGDQLVSEANRDLSSSL
                     RGDVVEARRESRAARDALENREAFIGASFDHIAGGGLRGRNIAIVSSGELPGSVESGV
                     RDAVQDADGGIASVSELVAPPEPAVLAEVAGRGFDDLRPGDPRMAALGRRIGRALANG
                     GALASRLADRFPERFRGDFPRVDAVAYYRDPGAERSDDEERFEQGLIDGLRAGRDPVV
                     GVEELDTEPSQIGFYEDRMQASVDSVDSPGGRIALVLTLRGARGSFGYKDSADAPLPE
                     PDGR"
     gene            32342..33178
                     /locus_tag="VF517_04785"
     CDS             32342..33178
                     /locus_tag="VF517_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052287.1"
                     /translation="MHALPLAISLLTAAVVAPPLVRGLREQGFVRENYRGEKVAFPAG
                     IAVPVVALLALVPLSLIAELADEEVFRPGTQAAVTYVIGVALLGLLDDLVGSDAPARG
                     WRGHARAAATGRLSTGAIKAVGSLGLALYALSGAGRDTGEYLLAVVVLVLATNLFNLL
                     DLRPGRSAKALVLLGLALSLGAMDADPLWTVGLFLGPILVLLPLDLREVGMLGDTGSN
                     AIGAVAGLWLVLTLSVTGQAIALAVMAALTIYGEFRSISAVIDRTPGLRHLDSVGRTS
                     HA"
     gene            33171..34886
                     /locus_tag="VF517_04790"
     CDS             33171..34886
                     /locus_tag="VF517_04790"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX8052288.1"
                     /translation="MPNSRSQTRFIFVTGGVVSALGKGIAAASIGRLLVARGFSVTLQ
                     KFDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDANTTRGSNVTTGAIYN
                     QVIRRERRGDYLGGTVQVIPHITDEIKNRIRLVAEAAEVDFVITEIGGTVGDIESLPF
                     LEALRQFQVDQGRDRSMFVHLTLVPFLGHAGELKTKPTQHSVQELRRIGIQPNMLLCR
                     SEGALSREIREKIALFANLPVDAVISARDVDSIYKVPLYFRAEGLDDQILDHFRMEAP
                     PADLADWEALVKRGDSAEETVRIGMVGKYIQLEDAYLSVIEALGHAGLHNGARVEIRW
                     VDAEGAESGEAERQLEECDGILIPGGFGVRGVEGKIRAARFARERGVPYLGICLGMQV
                     AVAEFARHVAGLDGANSTEFDPETPFPVIDLLPEQKDVRDMGGTMRLGADPVKLHEGT
                     AARSIYAEPVVYERHRHRYEVNNFLRKRLEHAGLVCSGTSPDDRLVEIIELPDHPFFV
                     ASQFHPEFKSRPLRPQPLFRDFVAASLERSREGSSKGEGRFARDGEAATEPGAPQTSR
                     TALTD"
BASE COUNT         4639 a        12694 c        12946 g         4691 t
ORIGIN      
        1 gctccggcgg cgctgcgctc cccggcggca gcggcatggc cggcggctcc ggcgccagca
       61 cgcccggtgg ctccggtggc tccggtggcg gcggcacgcg ctacttcacc tacacggtgg
      121 acgtgaagtt cggcagccgc aagcccaaga gctacaagtc gctcgccacg ctcgacctgc
      181 tccccgacga ccagaacccg atcgtgtcct tcatgggcat gaccgacaac gccaagaccg
      241 cggtgttctt cgtggtggac ccggccttcg aggccgacgg cgagggcaag tgcagcccga
      301 gcgccgacga ctgccgcttc atctacctcg gggtcgagga cgaccgcgac gaggagacgc
      361 tgtcggcagc cgagggcgcg ctcgagtaca cgctccagct cgacaggatc aacatcaagc
      421 gcctcagcga gaacgaggcg gtcggcaacg cccagccgga gagcgacact cccgacaccc
      481 ccaccgcggc caagcgcagg cagagccgca agaccctgat gaccgtgccg ctggcggcac
      541 gccgctagac cttcccccca acccccccgg acgggaagta cagccagcgc ccgagggccc
      601 gccatacggc gggtcttcgg cgttccaggg actatgcggt ggagcgccgg ccctctgtgc
      661 tgaagaggat cgcccggccg accaggatgc cgacgagcgt cacggcgacg ccgaccacca
      721 tcaggacgat cagctcggtg tcgaagacgt ccatcaggcc ggtgtagagg cccacgatga
      781 tcggcagcac caccggtagg agcgcccagc ccgagggcgc cgcgtagccg aacacgatgc
      841 cgcagagcag gaagagtgcg aagccgatga tcatcgcggc ggaggttacc tgtccgggta
      901 gggttgtcgc gtggtcgcct ttcgcttcac gacagccggc gagtcgcacg ggccggggct
      961 cgtggcgata gtcgaggggc tcccggccgg cctcgagctg gcccccgcgg acctcgacgc
     1021 cgacctggcc cggcgccagc tcgggcacgg tcgcggcggg cggatgaaga tcgagaccga
     1081 ccgcgccgag gtgacggcgg gtgttcggca cggcaagacg ctcggcagcc cgatcgccat
     1141 tcgcgtggcc aaccgcgact acgcgaactg ggaggagcgg atgaacccgt ggccggtcga
     1201 cggccagccg gacgaggtgc acctgccgcg ccccggccac gcggatctcg cgggggtgca
     1261 gaagtacggg ctgaccgacg tgcgcgacgt gctcgagcgc gccagcgcgc gcgagacggc
     1321 cgcgcgggtg gccgcgggcg ccctcgccaa ggcgttcctg cggcgcctgg gggtggaggt
     1381 gcgcagccac gtgatcgcga tcggctcggt gaccgcgcct gctcccgacg acctcacggc
     1441 cgccgacttc gagggcgtgg acgagtcgcc ggtgcgctgt ctcgacgccg actcgaccgc
     1501 ggcgatgatc gcgtacatcg accaggcccg gaaggcgaac gagtcgctcg gcggcatctt
     1561 cgaggtgcgc gctttcgggc tcgtgcccgg gctcggctcg cacatctcct gggagacgcg
     1621 cctcgacggc ctcgtggcgc acgcgatcat gtcgatccag gcgatgaagg gcgtgtcggt
     1681 gggcgacgcg ttcgacgtgg ccacgcggcc ggggtcgcag gcccacgacg agatcttctt
     1741 cgacgacgag cacggcttcc accgcgagac caaccgcgcg ggcggcctcg agggcgggat
     1801 gacgaccggc gacccgctgg tggtccgcgg ggcgatgaag ccgctgccca cgctgaccaa
     1861 gccgctgcgc tcggtcgaca tcgccacgaa ggagccggcg caggcgctgc gcgagcggac
     1921 cgactcggtc accgtgccgg cggcggcggt cgtcggcgag gcgatgatcg cgatcgttct
     1981 ggcgcgggcg taccgcgaga agttcggcgg tgaccacatc gacgacgtcg cgggcgcact
     2041 cgacgcctac cggagccgga tcgggtggag gcggtagcgg caggcggctc ggcggcgggc
     2101 gccgccgccg gcgcgggccg gcgcgcgctc gtcttcgtcg gcttcatggg tgccggcaag
     2161 tcgagcgcgg cgcgtgcggt ggccgcggag ctcggcgtgc ggccggtcga ctccgaccgc
     2221 gagctcgagc agtcgctcgg ccagccgatc gagacgttct tcgaccgcca cggcgaggcg
     2281 gagttccgcc gccgcgagga ggagctgatc gtcggcctgc tcgcgcgcgc cgacggcggc
     2341 gtcgtcgcgc tcggtggcgg cgcgctcggg tccgatcgag tgcgcgaggc gctcgccccg
     2401 cacgtggtcg tgcacctcga ggtgagctcc gacgccgcgt ggcgccgggc gtccggcaag
     2461 ggccggccgc tcgcgcgcga ccgcgcccgc ttcgaccagc tcttcgccga gcgccgcgcg
     2521 cgctacgagg ccagcgcgga cgcgatcgtg ctcgcctccg accgcggcgt ggccgcgcgc
     2581 gccctgccgt cgctgctcgc gctgcgcgac ggcccgcccg gcacccgttg cgtatgggcc
     2641 tcgggcgggg cggccgagta cccggtcttt ctcgggcgcg gcctcctcgc ttccggcttc
     2701 tttccgccgc tcggcggccg gcgcttcgcg gtgagtgacg agaacgtcgc gcccctgcat
     2761 cgcgtggagg cggactggag ctgggagatc ccgcccggcg agtcggagaa gacgctcggc
     2821 cgcgcggagg cggtgctgcg cgggatggcc tccgcggggg tcgcgcgcga cgacctcgtg
     2881 ggggccgtcg gcggcggcgt ggtgggggac ctggccggct tctgcgcggc cgtctaccag
     2941 cgtgggatcc ggcacgtgca ggcgcccacg acgctcgtgg cgcaggtcga ctcggcgtac
     3001 ggcggcaaga ccggcgtcga cctccccgag ggcaagaact acgcgggcgc ctaccaccag
     3061 ccgtcggccg tgatggtgga cccggccgcg ctcgacacgc tgcccgcggc ggagcgcgcc
     3121 gccgggtacg cggaggtcgt caagacggcg ctgatcgcgg gcggcgagct gtggtcgcgc
     3181 gtgcgcggcg gctccgagcc ggacgcggcg atcgtcctcg gctgcctgcg cacgaagctc
     3241 gccgtggtcg ccgaggacga gcgcgacgag ggccgccgcc aggtgctcaa cctcggccac
     3301 acgatcggcc acgcgatcga ggcggccacg gggtacgagc gctttcgcca cggcgaggcc
     3361 gtcgcgatcg ggctgctctg cgcgctgcgg ctgtcggggc gcgagcagct gcggagcgag
     3421 gtggcggagc tgttccgcgc gcgcgggctc ccgctgagct gtccgggcgt ggacgctgac
     3481 gctgtggtcg agctggtcgc gcgcgacaag aagcgccgcg gcggcagcgt gccgttcgtg
     3541 ctggtcgagt cgcccgggca ggtgaccccc gggcacgagc tggcggagga cgcggtgcgc
     3601 gccgccgtcg aggaggtgtg tgcggaatga agaaccgggt ggaggtgatg cacggggtga
     3661 acctcgatca gctcggcgtg cgtgaccccg ccgtctacgg cagccagacg ctgaccgagc
     3721 tggaggtgcg cgtgaagcgc ttcgcgcaag agctcgggct cgagaccacg ttctcccaga
     3781 ccaaccacga gggcgagttc tgcgaggggc tgcacgaggc gcgcggcacg gtcgacgggc
     3841 tgatcctcaa cccgggcgcg tggacgcact actcgtacgc gatccgcgac gcgctcgagg
     3901 tgacggcgct gcccgcggtc gaggtgcaca tctccaacat cgagggccgc gaggactggc
     3961 ggcgggcgtc ggtgatccgc gacctctgca tcggctacgt gcacggccgc ggcgtcgacg
     4021 gctaccgcga cgcgctcgag atgctgcgcg ccgagctggg aggcggctcg tgagccgggc
     4081 cgaccgcgtc gaggcgctgc tcgccgacgc ggagctcgat gccctgctcg tcaccaacct
     4141 cgagaacgtc cgctacctga ccggctacac cggcacgaac ggcgtgtgcg tgatcggccc
     4201 ggacctgcgc gcgttcttca cggacttccg ctacgtcgcg caggccgagc gccaggtgca
     4261 cggcttcgac cgcgcactcg gcaagcagga cctgctgggc gacgcggccg ggcggctgtc
     4321 gggtcgggtg gggatcgagg actacgacct gagcgtgcgc cgccacgagc ggctgcgggg
     4381 gctcgtgggg gagggcgtcg agctcgtgcc ggccgggcgt tgcgtggagc gcgtccgggc
     4441 ggtgaaggag cccgaggagc tcgaccgcgt gcgcgcggct gccgcgctgg ccgacgagct
     4501 gttccgcggg gtggtcgagc gcgggctcat cgggcgaagc gagcgtgaga tcgccttcga
     4561 gctcgagacc gagatgcgcc ggcgcggggc cgcgccgtcg ttcccggcga tcatcgccgg
     4621 cgggcccaac ggggcgctgc cgcacgccga gccgggcgag gacccgatcg cgcgcgacac
     4681 gctcgtcgtc ttcgactggg gcgtggagct cgacggctac tgctccgact gcacgcgcac
     4741 gctcgcgacc gggtcgatcc cggacgacct ggcggaggtc tacgagttgg tgcgcgccac
     4801 gcaggtcgcg accctcgacg aggtgcggcc gggcgcggcg ggcgcggcgg tcgacggctt
     4861 cgcgcgcgag cggatcgcgg ccggggggca cggcgagcgc ttcggccacg ggctcggcca
     4921 cggggtcggg atcgtggtgc acgagggccc gacgctgtcg aaggcgtcgg aggacaccct
     4981 cgtcgcgggg aacgtcgtga cggtggagcc cggcgtgtac gtgccggacg cgttcggggt
     5041 gcggatcgag gacctggtcg tcgtcgccga cgacggtccg gagatcctta gcggcttccc
     5101 gaaggggctg acggaggtcg gctagtaggg ggctggcggc ggcgggctac cagaccccgc
     5161 tgcggcagcc gcccttcttg ttcgtcgagc ccgccgtgca ggtccggtcg ttcacgccgg
     5221 aaacgccgtg gtcgatcgtg taggtgtggt tcgagccgtc gctctgggcc ttcgagatcg
     5281 cggtgacctt gaaggtcgag gtggtcgcgc tgaccacgct gacctggccg ggggcggtgc
     5341 cgtagggcag gttggtgtcg gccccgagct cctcgttggt gtcgcagttc gtgtagttct
     5401 cctcggtcgc gtagcagagc tcgacgcgtg agaccaggtt gcgcgcgttc gactccgcgt
     5461 cggagtcctt gcccttcttc tggtgctcca ggtaggccgg gagggcgatc gcggcgagga
     5521 tcgtgaggag caggaccgtg acgaccagct cgatcagggt gaagccgcgg cgtgagcgca
     5581 ggcgcagggc gagggagctg aacatgcggc tgtatcggta aatcgggcag cggtctttag
     5641 cgccgccgcg ccgcgttgtc cggccgtttg cagggagtcg tgccgccgtc ctccacctat
     5701 gcgaacatgc gttcgtatga cggtctgcgt cctctatccg cggttcgcgc tcctggcggc
     5761 catctcggag cgcgacgagc tgctggcggc gccggtggcg ctggctcccg caccgggcgg
     5821 gacccaggtc gtggggcagg tgtcggcggc ggccgaggcg ttcgggatcg cggccggcat
     5881 gagggtgggg gaggcgctgg cgcgctgccc cgagctgcgg ctcgtgccgc cggaccccga
     5941 gtgtgagcgg gcggcgtggg ggcgggtgct cgaccggctc gagtcgatcg gcgcggcgtc
     6001 cgagtcggac gtgcccggta tcgcgttctt cgaggacgag gggctgcgcc ggcttcacgg
     6061 cgggcaggtc gacgacgtgc tgaccgcgac ccgccgcacg ctgggcggag cggtgcggct
     6121 gggctgcgcg ccgagccgct tcgcgtcgta cgcggcggcg ttgcaggcgc ggccaaggcg
     6181 gcgcgggggg cggggccgcg cgccgggcgg cggctccgtg ccgcgggcgg ccggtggtgg
     6241 caccggcatc gctacgcgcg gtgcgggcgg cgccgtcttc gttccccccg gcgcggtccg
     6301 cgccttcctc gcgccgctcc ccgtcgcgct gctgcgcacg cggccggagc tcgccgcctt
     6361 gccggacacg ctcgagcggc tcggcgtccg cacgctcggc gagttcgccg cgcttccgcc
     6421 ggcggcggtc tccgagcgct tcggccatcc gggcctgctc gcactggacc tcgcgcacgg
     6481 ccgcgacacg gcgctcgacc cacgccgccc gccggagccg gtggtcgagc gactcgacct
     6541 gcccgaggcg gcgctcggtc cgcagctcga gcgcgcgctc gagctgctcg tcgcgcgcgt
     6601 cctcgcccgg cgcgagcgcc gcggccggac ggtgcgagcg ctcgcccttg tcgcgcgctt
     6661 cgtcgagggc ggcacgtggc gggtgcgggt cacgctgcgg cggccgagcg ccgaccccga
     6721 ccggatcctc accgccgtgg gcgtgaagct gcccgagctg ccggcaccgg ccgagtcgct
     6781 cggcctggag gtggaggcgt tcggcccgcc cgcgcacgac cagggccgcc tgctcccgga
     6841 cactcccgcc gagctgcgcc gccgccggat agccgaggcg gtaggccagg cgcgccaggc
     6901 cgcggggccc gacgccgcga tgcgcgtgct cgaagtggac cccggctcac ggctgccgga
     6961 gcgccgagcc gtgctggccc cgttcccagt ggaggagaac ggagacggac ccgcgccgcg
     7021 tgggaggccg gcttcgcgcg gcggatccag cccgcgtggc ggctccgctc cgcgtggctc
     7081 cgcgcgcggc ggtcccgccc cccgcggtgg ctccgcccca ggcggcaagc gatgagcgtt
     7141 cgccgcctcg cgcacccacg cggcgtctgc gtccgggtcg acgacgcggg cgctcccgcc
     7201 gccctccagg gcgagcccgt ggactcggtg ctcgaggaat gggtggtcga ggaccgctgg
     7261 tggaccgacc gcccggtccg ccgccgctac ttcgagcttg tgctggcggg cggccgcaac
     7321 gcggtggtgt tcaaggagct ggggggacgc gggtggttcg agcagcgggg gtgagtgacg
     7381 gcgggggagc ccaaagccac tccgaccatc accaccgcat cgattggagc ctttctcccc
     7441 tatagacagg aaaagcctcc aatcgatggg aaatggccgg acggagacgc ccgccgccga
     7501 tcagagactc cggcgcgagg cggagacgca cgccgccgcg gagccgagct gccgcccgcc
     7561 gggagctccc gcccgccggg tctcaacccc accgcttgag cccaagccca gggcatgcga
     7621 acatatgttc gtctcccatg ccctacgtcg agctccacac ccactccgcc ttctcgttcc
     7681 tagacggcgc ctcgcacccc gtcgagctgg ctgcagcggc cgccgagcag ggccacagcg
     7741 cgatcgcgct caccgaccac gacgggctgt acgggtcgat ggagctcgcc atcgcggcca
     7801 agccgctcgg cctgcgcgcg atcgtgggcg cggagctcac gctcgacgac ggccaccacc
     7861 tcacgctcct gtgcgaggac cgcaccggct acgccaacct ctgccggctc gtcacgaacg
     7921 cgcacgcggg cacgcgcgag cgcccggccg accccgtccc gccgagcacg agcctcgagg
     7981 acgtcgagcg ctttgccgag ggtctcgtgt gcctgtcggg ctgcgcgcgc gacggcgccg
     8041 tcgcggcgcg catagagcgc gacgagcacg cggaggcggc cgccaccgcc aagcgcctca
     8101 ggcgcgcgtt cggccccgac cgcttccgca tcgagctcca gcgcccgttc gaccgccacg
     8161 accgccgccg caaccgcctg ctcgcgcagc tcgccgagcg catcgggatc ccgtgcgtgg
     8221 ccaccggcaa cgtccacgcc cacagccggg agcgggccac gctccaggac gcgttcgtcg
     8281 ccgtgcgcac cgggtccacg ctcgacgagt ccgagccgat ccgccgcggc aactcgtcac
     8341 acgtgctcgc cccgccgaac cagatggcgg cgcgcttcgc cgaccacccg gacgccgtgg
     8401 aggagtcggg ccgcctggcg gagcggctga ccttcgacct cacgaccgac ctcggatacc
     8461 gctacccggg agcggaggga ggcagcgccg accacgacct cgccgagctc tgccgctcgc
     8521 tgctgggcag ccgttacgag ggccgggcca gccgcgccgc ggcggagcag cggctggaga
     8581 aggagctcgc catcgtccgc tcgctcggcc tcagcggctt cttcctgctc caccgcgacc
     8641 tgctcgagtt ggcccgcgag gtcgcggccg aggtgcgcgg ccccgattcg gcccgcgcgc
     8701 tgctgccgcc cggccgcggc cgcggctcca gcgtctcgtc gatcgtctgt tacctcaccg
     8761 gcctctcgca cgtggatccg gtcgagaacg acctcttcct cgggcgcttc ctcaacgagg
     8821 acctcagcgc tctccccgac atcgacctcg acttcccgcg cgacatccgc gagaccctca
     8881 tcccgcgcgt gcacgagcgc tacgggcacg accgctcggc cctcgtgtgc gcgttcgcca
     8941 cctaccagac ccgctcggcc atccgcgact tcggtaaggc gctcgggctg cccgccgggg
     9001 agatcgagcg cgcggcgcgg gcggcggagc cgtggggtgg gggcgacgtc gagccgcacc
     9061 tggtcgacgc gatcggccgc cgccgcgccg cctcgccgcg ctggcaggcc ctggtccggc
     9121 tgacccgcga cgcctggggc ctcccgcgcc acgtgaccca gcacgtcggc ggcatggtga
     9181 tctcgaccca gcccctgaac gagatctgcc cgatccagcc ggcggcgatg agcggccgcc
     9241 agatggtgca gtgggacaag gactcgtgcg gcgacgccgg cttcctgaag atcgacctgc
     9301 tcggcctggg gatgctgtcg gcggtcgagc gctgcgtcga ccagatcgcc cgcgcgcgcg
     9361 aggagcggat cgacctctcg cggatcccgt tcgacgatcc ggcggtctac cgcgagatcc
     9421 aggccgcgga gaccatgggc gtgttccaga tcgagagccg cgcgcagatg cagatgctca
     9481 agcgcacgcg gcccgagaac ctcgacgacc tgaccgtgca ggtcgcgctc gtgcggcctg
     9541 gcccgatcca gggcggggcg gtccacccgt acatcgagcg gcgcaaggcc ctgcgcgccg
     9601 atcccggcta cagggtcccc tacgaccacc cctcgctcga gccggtgctg cgcgacacgc
     9661 tcggcgcgat cgtcttccag gaccaggtga tcgaggtggc gatggcgttc gccggcttca
     9721 cgcccggcga ggccgagggc ctgcggcggg cgatgagccg caaccgctcg gtggccgcga
     9781 tgcgctccta cgaggtcaag ttcgtcgagg gggcgatgga gcgcggcgcc tcgctcgaca
     9841 ccgcgcggaa ggtctacgcg cagatcgtcg gcttctccgg gttcgggttc ccgaaggcgc
     9901 actcggcggc gttcgggctc ctggcctacc agtccacctg gctgcgcgtg cactacggcc
     9961 cggagttcct gtgcgcgctg ctgaacgagc agccgatggg cttctaccca ccagactcgc
    10021 tggtgcacga ggcgcagcga cgcaggattc cggttctccc cccgtgcgtg ctgcgctcgg
    10081 aggcggagtg ctcggtggag cgcgtcgagg gcggcgcgct ggccgtccgg atcggcctcg
    10141 gctacgtggc cggcgcgggc aaggaggacg tggcggaggt cgttgccgag cgcgagcgca
    10201 gcgggccgtt ccgcggcatc gccgagctgg ccggtcgctg cggcgcgcag cgcgacgcgc
    10261 tcgagcggct cgcctgggcc ggcgcctgcg acgcgctcgt cgaggacggg cgccggcagg
    10321 ccctgtggct gctcggcgtg tcggcgcccg gcgtgaccgt ggcgggcggg acgcagctcg
    10381 cgctccccgt ggagcccggc gccgcaccgg agctgcgcga gctctcgccg tgggagcggc
    10441 tgatcgcgga ctacggctcc acgaggatca cgctgcacga gcacccgatg gagctgatgc
    10501 ggccgagctt cgacgccggc ctcgtcacga gctccgccct ggccgccctc ggccacgggg
    10561 cgcgcgtgcg caccgccggc ctcgtggtcg cccgccagcg ccccgcgacc gcgaaggggg
    10621 tcacgttcat gctcctcgaa gacgagcacg gcgcgatcaa cgtgatcgtc gcgccaccgg
    10681 tctacgaggc ccaccgcctc gccgtccgcg ccgagccgct ggtgtacgtg gagggccggc
    10741 tcgagcaccg cgagggcgtg acgaacgtgg tcgcgaccgc cgtgcaccgc ctcgagcggc
    10801 ccgacatccc gctcggcgag gtccgccaca tcgagccgcg ccgcgcctgg agcacggagg
    10861 aggcggcggg cgacctacga gcggtcgccc cgacggcgca cagcttcggc cggcggggct
    10921 gatccggcac ctggggggtt tcctccccct gtccgggggg tgaaccagga ctagggctag
    10981 tacaaagtgg gtacaggcga acgcggtgcg gaggcggggt ggccgtctcc cttcggggct
    11041 aaggggctat atgcgtaacc gcggactgct gctgctcgcg ctggcgatcg tcgccgcgct
    11101 gtcgattccg tccacctcga gcgcggcgac cgggacggtc atcccgggcc actacatcgt
    11161 ggtcctcgag gactccgccg acgtcgacgc gatggttgcc gaacacgtcg acctcggcgc
    11221 ggtcgtgggc cacacctacc gcagcgccct gcacgggtac gcggcacgct tgtcgcccac
    11281 catccgcgcg ctcgtgaagg ccgatccgcg cgtggcacga gtggtgcccg accgcgaggg
    11341 cgtcgcgacc caggcgcaga cgctccccac cggcgtgaat cgcatcgacg ccgacctcgg
    11401 cagccaggcc gcgggcgacg ggaccggcac caccccgggc gccgtcgcga tcatggacac
    11461 gggcatcgat acgacgcacc ccgacctcga cgtggccggc ggcatcaact gcctcggctc
    11521 ctacagcacc cacgacggaa ccatcggcga cgagaacggc catggcacgc acgtggcggg
    11581 gatcgtgggc gccaaggacg acggcgaggg cacggtgggc gtcgcccccg gcgtgcggct
    11641 ctggtcggta cgagtgctca gcagcaccgc ctcgggcacc acctcgacgc agctctgcgg
    11701 catcgactgg gtgaccgcga acgcgcaggc gctcgggatc aaggtcgtga actcgagcat
    11761 ggtgctcttc ggcgcggtcg acgacggcaa ctgcggcaac accagcggcg acccgctcca
    11821 gcaggcgatc tgcacctcga ccgccgcggg gatcctgtgg gtcttcgccg cgggcaacag
    11881 caacgccagc ttcgcgggtt ccgccggccc gagcttcgac caggtcctga ccgtcaccgg
    11941 cctggccgac ggcaacggcg tgcccaacgt gccgaccacg accacgttca cctgtccggc
    12001 cgtcaacggc acgcgcaagt acccgaacta cgcgccggag accgacgaca agacctactc
    12061 ctattcgaac tacgccgtga gcggcgatgc cgcgcacacg atcgccgcgc ccggctcgtg
    12121 catctggtcg acctacaagg gcggcaccta cggcaacatg accggcacga gcatggccgc
    12181 cccgcacgtc acggcggccg cgcacctgtg catcctgtcc ggccagtgcc cggggacccc
    12241 cgcggagacg atccagaagc tgcgggccga cgccgaggcg ttctcgctgg gcgatgccgg
    12301 gttcggcttc acgggcgatc cgctgcgccc ggtgaccggc cgctactacg ggtacctcgt
    12361 gcgcgccggg ctgtactagc taccgtcccg cgggatggcg ggacggatag tcgttggcac
    12421 gtcgagctgg gccgaccccg gcttcgtcga ggagtggtac ccgcccggca tgccggcgcg
    12481 cgagcggttg ccctggtatg cggagcggtt cgagtgcgtt gagctgaact cgagcttcta
    12541 cgcggtgccc gatcccggcc tcgtcgagcg ctgggtgcgg gcgacgccgg acggcttcgt
    12601 gttcgacgtg aagctccacc ggctgctctc gcgtcactcg gccgacctga agtcgctccc
    12661 gcccgacctg cgcgaggagg cgcagaccaa cgagcgcggc cgcgtgcgcc tgacgcccga
    12721 gctcgagatg gcggtggcgg accgggtgct cgaggcggtc gcgccgctcg agcgcgccgg
    12781 caagctcggc gcgttcctgc tccagctcac cccggcgttc tcgcctgaca agcaccggct
    12841 cgacgagctc gacgggctgc tcgagcgcct ctccccgcgc gtcgtggcgg tcgagctgcg
    12901 caaccgcggc tgggtctccg acaagcgcgc gccggagacg ttcgcctacc tgtccgagcg
    12961 cggcgccgcg ttcgtgtcgg tcgacgcccc gcgcgaggag cacgtgccga tcatgccgcc
    13021 ggtcgacgcg gtgaccaacg aggcgctctc ctacatccgc atgcacggac gcgacgcgca
    13081 cggctacatg cacggcaaga ccgtggccga gcgcttcgcg tgggactact cggacgagga
    13141 gctggaggag atcgccgggc gcgtgcgcgg gctggccgag caggcgcacg aggtgcacgc
    13201 gctcttcaac aacaaccgcg gggccgacgc gccctcagcc gccagccgct tccgcgcgct
    13261 gatcggccag gatgccggcc cgccgcccga cgtcggccaa cagctccggg tcctgtgagc
    13321 gcacccgtcc gcatagcgac cggcctcgcc accgacgacg cgggccccga cgccttcgcc
    13381 gaggccgcgg cgcgcgcgca gctcgggctc ggcggcgcac aggccgacct ggtcgtcgcg
    13441 ttcgcggctg cgccgaacct cgaccacgcg gaggaggggc tggcggcggt ggaggagcgg
    13501 ctcggcccct acgcgctgat cggctgcggc gcccagggcg tgatcgccgg cggccgcgag
    13561 ctcgagacgg gcggggtcgc ggtgtgggcc gcctcgctgc ccggcgcgac gctgcggccg
    13621 ttccacctcg acgcgttcga cgccggcgac gagggcctcg cgatcgccgg gctgccgggc
    13681 ctgaccggcg tggaggccgt cctgctgctc gccgacccgt acaccttccc ggtcgagccg
    13741 ctgctcgacc gcctggccgc cgaccacccg gcgctccccg tgatcggcgg gctggcgagc
    13801 gcgggcgcgg tcccgggccg ggccacgctg ctgcacggcg gcggcgtcga ggagcgcggc
    13861 gcggtgggcg tggcgctggc gggggtcgac gtgcgcccgt gcgtctcgca gggcgcgcgg
    13921 ccgatcgggc cggagatggt catcaccgcc tgcgacgaga acgagatcca cgagctggcg
    13981 agcaggccgg cgctggcgcg gctgcacgag gcgatcgggg agctcgaccc ggaggagcgg
    14041 gcgctggccg gccgcggcct gctgctcggc atcgtgatcg acgagaacca gcctgactac
    14101 gagcgcggcg acttcctgat ccgcggcctg ctcggcatcg acgaggagaa cggcagcgtg
    14161 cgggtgggcg agcgcgtgcg ggtgggccag acggtgcgcc tccaggtgcg cgacggcgag
    14221 tcggccgacg aggacctgcg cgaggcgctc gcccgccacg ccgacgccct gagcggcccg
    14281 ccggcgggtg cgctgctgtt cagctgcaac ggtcgtggca gccacatgtt cggcgagccc
    14341 gaccacgacg cgctcgcgct cgacgacgcg ttctcgggcg cacccgccgc cgggttcttc
    14401 tgcgcgggcg agatcggtcc cgtgggcccg cgcagcttcg tgcacggctt cacggccacg
    14461 ctcgcggtgt tcgcgggctc gggaccgggc tccgcgatct aggcgagggc gagcgcgggg
    14521 cggcggtccg cccacggccg cgccgcctcg atctgcgccg cgagcgccag cagcagctcc
    14581 tcgccggcgg gcgggcccat gagctgcacg ccgatcggca ggccgctggc cgacacgtgc
    14641 agcggcagcg cgatcgcggg ctggccggtg acgttcgcgg ctgcgctgaa gggcacgaag
    14701 cggctgccct tccagaacgc ctcgagcccg ggctcccctg gtgcggcggc gagcgctccg
    14761 tgcgggacgg gcggctgggc gagcgccggg gtcaggagca cgtcgtggtc cgcccaccag
    14821 cgcgtcaccc ggcgcgtgta ggcgcgcagg gtcgtgaggc cctcgagcag gtcgcgtgcg
    14881 gtccggccgc gcgcgttctc gatcagcgcc tgcgtgagcg gctcggtggc gctcgggtcg
    14941 agcggcgccc cgcccagcgg cgcgatgcgc tccgcgccga gggacgcgtc ggtggcccac
    15001 atggcgagga aggcgtccag gtgctcgcgc gcctcccact tcggggcgct ctcctcgacg
    15061 tcgtggccca gctccgcgag cagcgccgcg gtcgcggtta cggccgcgac gcagtcggcg
    15121 tgcacctcgg cgtccacggg aggcacggtc gtgacgccga cgcgcaaccg cgcgggcgcg
    15181 tggccggcgg cttcgaggaa ggtgctccgc ggcggcgcgg gcgtgacggg gtcgccccac
    15241 tcgtagcccg cgagcacgtc gagcgccgcg gccgtgtcgc gcactgtgcg tgttaggacg
    15301 ccctcggcga ccagcgccga cggcgagtcg acgagcggcg cggagctcac ccggccgcgg
    15361 ctcggcttga ggccgacgag gccgcagcag gacgcgggga tgcggatcga gccgccgccg
    15421 tcgtttccgt gggcgaggct caccatgccc gcggccacgg cggccgcgct gccgccggag
    15481 gagccgccgg ccgtgcgcgt caggtcccag gggttgcacg tcgggccgaa gcgcgcgggc
    15541 tcggtgaccg gcgtgatgcc gagctcgggc gcgttcgtct tgccgacgat caccgcgccc
    15601 gccgcgcgca gcttcgtgac gagcgcgctg tcgaacggcg ggatccagtc gccgctcagg
    15661 gacgtgccga acgtggtccg gatcccttcg gtgacgggcc cgaggtcctt gatcgccatc
    15721 gggaccccgg cgagggggcg tgcgtcgccc gggccgatcg cgtcggcctc ggccagcgct
    15781 ctctccggca cgagggtcac gaaggcgttc acgtcgccgt cgaggcgctc gatggcagcg
    15841 agcgacgcct cgaccagctc acgcgcgctc acctctcccg cgcgtacggc cgcggcctgc
    15901 tcgagcgccg gacgctggat cggctccgtc acggcctcga ccctagtccc tgcaaccatt
    15961 cgggcgtgag ctccgccgat cgcgcgccag acggagtcgt gcgcgtgcgc gcgccgaacc
    16021 cgtcgccgct gacgctcgac gggaccaaca gctacgtcgt cggccgctgg gtggtcgatc
    16081 ccgggcccga ccacgccggg cacctcgatg cgctgctcga tgcggcgggc gggcggctcg
    16141 acgggatcgt gctgacgcac gagcacatcg accacgccga gggcgcgcag ccgctggcgg
    16201 agcgcgccgg gggcgtgccg gtcgtgcagc cgcgcgaggg ggacgaggtc ggtccgttcc
    16261 gcgcgatagc cacgcccggg cacgccgagg accacgtctg cctgctgatg gggagcgtgt
    16321 gcttcagcgg cgacacggtg ctgggggagg ggagcgtgtt cgtggggttc ggcggtgcgt
    16381 cgatgggcga ctacatggcc tcgctggagc gcctgcgcga gctggagctc gaggtgatgt
    16441 gcccgggcca cggaccgttc gtgtgggatc cacgcgcgcg ggtcgaggag tacatcgcgc
    16501 accggctcga ccgcgagcga cgcgtgctcg aggccctggc cgcgggcgcg cgtacgcacg
    16561 acgagctgct cgacctcgcc tgggacgacg tcgacttcac cgacgcgccg atgctgcgcc
    16621 tcgccgcggc ggcgacgctc gaggcgcacc tggagaagct ccgcggcgag ggccgcgcgc
    16681 cgcagagcgg ggcctgacga gcgcggacgg cgcttcgcgc gtcgggctcc gcgggcgccg
    16741 ggctccgccc gcggccgggc tccgcccgcc gggcacccgg ggcccggcgc cgccgggctc
    16801 ggccccccgg aactgggcaa cgttaggtgt caaatacgac caaaatgtcg cccacctccc
    16861 ggggccgcga cgctgggcgt tacgggagcg ttacgagtgt tacgggagca gcgcgcggaa
    16921 ctcggtgcgc aggctgcgac ggcgccggcg cctgcgcttg cggttgccgc tcacgcccac
    16981 cgctccgcgc agtcccacgc agccgatgca cccgacgcag ccctcgcagt cgacgcaccc
    17041 gacgcacccc acgcagccgg tgcagttcac gttcccgagc ccggccacca ctcccgcgct
    17101 cgccacggtc ccggcgctga agagcacgcc cgcgctggcc acgctcgccg cgccgccgac
    17161 cacgccggcg ctcgccacgg tccccgccga gccgatcaca cccgcgctgg ccaccgtccc
    17221 cgccgagccg atcacccccg cgctcgcgac cgtgcccgcc gcgccgacca cgcccacact
    17281 ggcgacggtg ccagcggcgt cctcgacgcc cgcgctggag gtgctcgggc tctccgtgct
    17341 cgggctctcc gtgctcacgc ggtggaacct accgggccat ccccgtacgg gaggccgttg
    17401 ttggtgttcc agaacaccac cggcccgccg cccgcggcgc ccgccagcag cgcggcgagc
    17461 gccttgcccg tgtagacggg gtcgagcgcg agtccctcgg catcggcccc gagcgcccgt
    17521 gccgcgtcgc ccgcggcggt ggcgtgcccg tagcccgcgc ccatgaagcc ctcgaccacc
    17581 cggaccgagc cgggatcgag ccgcgccccg gagacgtccg cgcctcggcg ccgcagcagc
    17641 cgcagcgtgc ggcccgcgag ccgcagcacg ctcgcctcgc tcagccgcag ctggtcgttg
    17701 acgagcaccg cgtggacacg cgtgccgagc ccggagagcg cgaacccggc cgccaggccc
    17761 gccgcgctgc cacccgagcc gagcgcgacc accaccgagc gcggctcggg cagctcgccg
    17821 ctggcgacct gctcggccag ctcgagcgcc gcctccacga agccgagcgc acccgtcggc
    17881 gacgagccgc ccggcgccag caggtacgga gcccgcggcg gccgccgctg cgagtggcgt
    17941 agcgcgatca gtggcgcggc gagggctgtg cggcgcgcgg tgccggtgag gtagacccgc
    18001 gacgccgccg cgcgcagacg cccgagctgg cgctcgacgt gctcatcgcg cggctgctcg
    18061 gccagcgcaa gcacgcagtc aatcccggct cgccgtgcgt agaccgcggt ggcgagcccg
    18121 tggttcgtgc cgatcccgcc gaacgtgagg atcgtccgcc gcccgcgggc acgcgcgtcg
    18181 gcgaggatcc actcgagctt gcgtggcttg ttgccgcccc acggctcggc gctgcgtgcg
    18241 tcgtccttga cccacacgtc ggcgcccagg cccggcgccg cgcgcaccgg cgtcggcagg
    18301 gagcagagcg ggatgcgcgg aagcgtggcc gccgtcggcg ggaagcgctc gtggatcagg
    18361 ctgcggccgg gcgcgctaga ggccaagccc gttgacgacc cgctgcgcgg tgaggaagtc
    18421 cgagccggtg cgctcggcgt agagcttcgc ggcgttcatg cgcttgccct cgcgcacgag
    18481 cgcgaccacc tccgggtcga cctcggcggc cggatcctcg atcccgaccc cggtccgctc
    18541 ggcaagcatc cggacctggc gctcgagccg ctcgagccgc tgcatgatct gcgcttccga
    18601 gggtgcgtcg cccatgcggg ggcgagccta ctgcgcggca atcgcggtgc cgacgcggtc
    18661 caggaacggc ccgagcgccg gccactcctc ggcctgtacc tcgaccatca tgccgccgct
    18721 tccgtggagc ggcctgacgc gcagccagcg ccgccccttg cggtcgggct cgtccacctc
    18781 gatccgcagg ttgagcacgt ggaaccgcat gctctccgac cccgggaacc cgaagacctc
    18841 cagcacccgg ccgtcgaacg cgtagagcgt ggtgccggcc cagacctcgt ccacggggcg
    18901 agtatctcgc acatacgctt ggcggatgga gtccgacttc gaccgcgcga cccgtgcggc
    18961 gagtgcgaac gagatgccgc cgccgttccg ggcggcgctg gacgagcacg ccgccctcgt
    19021 cggtatcgag gggccggtcc tgagcacggc gcgcgcggcg gccgtcacgg aatcgacccc
    19081 ccacaggcgc cgcctcctgc gacgccagcg cccgcacgtg acctggatgg tcgtctgcgc
    19141 ggacgtgctc gcggtcgtgg gcgacgcctc gggcgcgccg gtcggatcgc tctatcgact
    19201 cgcggagctg gaggccaagc cgttcagctc accgctggta caggacgagg gcctcgaagc
    19261 gaccgcgctg ccggtcggcg gcagcgagcg cgtgtcggtc ttcctgccgc tcgcacccgg
    19321 cccgccgcgc gacgcgatag cggccgcgct ggcgctagcc tgagcgcccc gatgatcagc
    19381 accccaatta gttcagggtg tcacggcggt ctcgtcatcg ggggttggtc gcaggatgtc
    19441 gaagcccgct ctgcacgcgt gttacagagg tcgagaacgc tcgacgcact tagctaaagg
    19501 cttggcgcac aagcatgcga cgattctggg acggctgacc cgaaggcgtg cttagtggtc
    19561 cgatgggtgc cgccgcgaga tcatcgcgcg gcgtcttctg ctcgggccag cctgccgcgg
    19621 aagtcactga gttccttggc cttgcgccga cggaagtccg agccgctcgt ccgcgcgcac
    19681 accgcgaacg cctgtgcctc cgcgatcatg aactgcggat tggagccttc gtagtggttg
    19741 acggagatca ccgagtaaaa cgagctgtag ggcgtcgcgc tcaccgggct gtcgaaggtt
    19801 ccgacgttgt gacctccgcc gatcacgttg aatccctcgg ggcactcggc acgcgaactc
    19861 gtaaccccgt cggcctcact gctcttaggg ccatcgacgc gccggacctc tccgacggcg
    19921 ccgccgttgc ggatgtcgtc ggtcgtcagg gatccgtccc gcacatcagc gccggttagc
    19981 gactcgttct tgacgtgcga cgacgtgacg ctgttctttg ccagcttcgt ggcggcatat
    20041 ccggtaccgc cgagcgcgca gaacaaggcg atgcccgcga tcgcgagcgc tggtgatggc
    20101 aagctcttca tgaagtcctg ctcctccctt aggtgccaca ccatcggccc agcacctttg
    20161 taaatacctt cccgccgacc gaaccggccg atgtgatttt tccataatcg gaaacgtcga
    20221 gccctcggag tggtgtcgcg accttccgcg gcggctcggg ccggccggtc ccgcagccgc
    20281 gcacccacgc cggccggctg gcgccgcgca tttcggcctg acggttcgcg ggcctccgcc
    20341 cgccccgccg ctagcctgag ccagcgatga tcagcaccaa ccagttcaag aacggcaccc
    20401 acatcgacgt cgagggcacg atcttcaaga tcgtcgagtt ccagcacgtg aagcccggca
    20461 agggcggcgc gttcgtgcgc accaagctca agcgcaccac cgacggcaac gtgatcgatc
    20521 ggaccttccg cgccggcgag aagttccgcc ccgtccgcac cgaaacgcgc aagatgcagt
    20581 acctctactc ggacggcacc gacgcccact tcatggacgt cgagagctac gagcagttca
    20641 cggtgccgga ggaggcggtc agggagccgc tccagtgggt cctcccgagc caggaggtcg
    20701 agatcctcta cgtggacgac gagccctccg acgtgcaggt gccggccgcc gtcgagttgg
    20761 ccgtggccga gaccgagccc ggcgtgaagg gcgacaccgc gtcgggcggc ggggacaagc
    20821 cggcgacgct cgagtcgggc gtgatcgtgc gggtcccgct gttcgtcaac gtcggcgacc
    20881 gggttcgcgt ggacacgcgc tcgggcgcgt acgtctcgcg cgcgtagaga gaggacgatg
    20941 cgccgctccg accagcgcag ggcggcggtc gtggcgctct accagcgcga cgtgaccggt
    21001 cgcagcctcg aggaaacgct cggccgcacg gccacgatct tcacccgcga gctggccacc
    21061 ggcaccgact cccacatcga agagctcgac ggcctgatag agcgctacgc ggaggactgg
    21121 accatcgacc ggatcgcccc gctcgaactg aacatcatgc gcgtggcgct ccacgagatg
    21181 ctccaccgcg aggacgtgcc ggacgaggtc gcgctggacg aggccgtcga ggcggcgaag
    21241 gagctgtgcg gcgccggcgc tcccgcgttc ctgaacggca tcctcggagc ggtgcagagg
    21301 gagcgcgtca cgtgagcgga ctcgacgacg ccgtggccga gctcgagcgc gccgcggcgc
    21361 ggctccgcga gcccgacctc ccgccggagg aggccgccga gctggtcgag cgctgcgccg
    21421 agatcgccgc gcgcgtgggc gcggagctcg accgtcgcgc ggatcccgat cgcgcggctc
    21481 ccgggcagga gacgctgctc tgagcgtcga gcccgcggcc gccacctacc cggtcgagtt
    21541 gcaggcgcgc gtggacgagt acctcgccgg cctcaccttc gcgcgcgagg gcggcgtggc
    21601 cggactcgag gaggcgatgc gctactcgct gctcgcgggc ggcaagcgca tccggcccgt
    21661 gctcgcgatg gcgacggccg aggcgctcga ccgcgacccg gccgacgtgc tgccgctggc
    21721 cgccgcgatc gagctgatcc acacctactc gctcatccac gacgacctcc ccgcgatgga
    21781 cgacgactcg ctccgtcgcg gccagccgac ctgccacgtg aagttcggcg aggacgtggc
    21841 gatcctcgcg ggcgacgggc tcttcgccga ggcgatctcc ctcgtcgcca ccgagcagca
    21901 gggcgagccc ggtcacgtgc tggccgcgct cggccacatg gtcgcggcgg ccggggtggg
    21961 cggcatggtc ggcgggcagt acctcgacat caaggccggc gaccaggcgg acctccgccg
    22021 cgtgcacgag ctgaagaccg gccggctgat cgccgcgtcg atcgagtgcg tattgatgct
    22081 tggaggtctc gccggacatg ccacaatccc gtggagccgg ttcgctgtgg agctgggtgt
    22141 gctcttccag atcgtcgacg acatactcga cgtgacaggg gacgaggccg cactcgggaa
    22201 gccgcagggg tccgacgagc gccagggcaa gcggacctac gtcagcgtgt tcggcctcga
    22261 ccgggcccgt gagctggccg cgcaggccca cgcgaaggca cgtgccgcgc tggcagaggc
    22321 ggacggccgg accggcacgc tcgaacgcac aacggactac atactcacga gacgaacatg
    22381 agcctgctcg acaagatcaa cggcccggcc gacctccacc agctctcgga gcccgagctc
    22441 caggacgtcg cacaggaggt ccgcgagctc atcatcgaca cgatcggcga gatcggcggc
    22501 cacttcggcg ccaacctcgg cacgtgcgag ctcgcggtcg cgctccacag cctgctcgac
    22561 tcgccgcgcg acaaggtgct ctgggacgtc ggccaccagg cctacccgca caagatcctc
    22621 accggccgcc gcgaccgtct cggcacgatc cgcaagtacg gcggcctcgc ccccttctgc
    22681 tcgatccccg agtccgagca cgacatcatg ggcgccggcc acgcctcgac ctccgtgtcc
    22741 tacgcggtcg ggctgaagga ggcgatgcgc cgcggccacg aggaggacgg gaaggtcgtg
    22801 gcggtgatcg gcgacggcgc gctcaccggc ggcgtcgcgt tcgaggcgct tcacaacgcc
    22861 ggcggcatgg acgtgccgat cgtgatcgtc ctgaacgaca acggcatgtc gatctcgccg
    22921 aacgtcgggt cgctgtcgcg ctacttcacc cggatccgcc tcaatccgga cctgcaccgc
    22981 gcgcgcgagg tggtcgagtc ggggctgatg aagatgccgg ccgggctggg cgagcgcatc
    23041 gagcgtctcg gccccgagct gaaggcgtcg atcaaggcgt tctgggcgcc gggcctgttc
    23101 ttcgaggagc tcgacgtggc ctacgtcggc gtggtcgacg gccacgacgt gaccgcgctg
    23161 cgcaagtcga tcggggaggc gctggaggcc gagcgcccgg tcgtggtcca catcaagacc
    23221 gtgaagggga agggcttcgc gcccgccgag gagggcgggc tcgagggtat ggagaaatgg
    23281 cacgcggcca agccggcgtc gatcgtcggc cgcgcggccg ccccgaagaa gcccgacgac
    23341 gccgcggccc cgcccaagcc gccgcagtac acgaccgtgt tcggcaactc gctcgtcgag
    23401 gagtgccgcc gcgacgcgcg cgtgtgcggg atcaccgcgg cgatgaactc cggcaccggg
    23461 ctcaacatcc tccagaagga gctgcccgag cgctactacg acgtggggat cgccgagcag
    23521 cacgcggtgc tgttcgcggc cgggctcgcg ctcgggggca tgaagccggt cgccgcgatc
    23581 tactcgacct tcctgcagcg cgcctacgac cagatcgtcc acgacgtctg cctccagaac
    23641 ctgaacgtgg tcttcgcgat ggaccgcgcc gggctcgtcg gcgacgacgg tccgacccac
    23701 cacggcgcct tcgacatcgc ctacatgcgc tgcctgccga acctggtgat gatggccccg
    23761 cgcgacgagg cgatgctcgc cgacatgctg cacaccgcgc tccgctacga cgacggcccg
    23821 gtcgccctgc gctacccgcg cggcgaggcc gagggagtgc cgctcccgtc gcgcccgcag
    23881 ccgatcgaga tcgggcgcgg cgaggtcctt cgcgagggca gccgggtggc gctgatcggc
    23941 tacggcttcg gcgtgtcgct ggcgcttgga gcggccgacc tgctcgagca gcacgacgtc
    24001 gagccgacgg tggtcgacgc gcgcttcgcc aagccgctcg acggtgagct cctaacccag
    24061 ctcgccaccg agcacgagct gctcgtcacg gtcgaggacg gggtactcca gggcggcttc
    24121 ggctccggcg tcggcgagct cctgctcgac cgcaacctgg ccgccgacac gcgcctggtg
    24181 cgcgtcgggc tgccggaccg cttcgtcacg cacggcaagc ctgcgctcct gcacaaggag
    24241 gtcgggctca cccccgaggc gatcgtcgag cgggtgctcg cggactcgcg cgagcgcggc
    24301 ggcgcgctgg tctagcgacc cccgcggccc cccagaaaat aagcgacccg ccgggagggg
    24361 cgggccgctt gagggaggag tgctgcgttg tggatcttgg ggggatgaag tcctccgaca
    24421 acgtacgcac gttcgcatcg gagttgtgtg cgagccgcca cagatccggc cgcggcgggc
    24481 cgcgccggta cgctcacgcg gtggtcaaga ccaggctcga tgcgctgctc gtggaccgcg
    24541 gcctgttcga gtctcgctcg cgcgccgcgg ccgccgtgat cgccgggtcg gtgcggatcg
    24601 gcagcgacgg gcgccgtgcc gcaaagccgg gccagatggt cggcgacgac atgccggtgg
    24661 ccgtggacgc cccgccgccc tacgtctcgc gcggcggggt gaagctcgcg aacgcgctcg
    24721 acgccctggg agtcgaggtc gccggccgcc gctgcctcga cgtgggtgcc tccacgggcg
    24781 gcttcacgga ttgcctgctc cagcgcggcg ccgagcacgt ggtggcgctc gacgtcgcct
    24841 acggcgagct ccactggcgc atccgcaacg acgagcgggt gaccgtcgtc gagcgctcca
    24901 acgcccgcgc gctcgagccc ggagcgctgc ccttcccgcc cgatctggtg gtggtcgacg
    24961 tgtcgttcat ctcgctcggg aaggtcctgc ccgcggttct gggctgcgcg gccgagcgct
    25021 tcgactgcct cgcgatggtc aagccgcagt tcgaggtcgg gcgcgagcgg ctcggcaagg
    25081 gcggcgtggt gcgctcgccc gagcacaggc gtgaggccgt cgcgacggtc gcccgcatgg
    25141 cggccgagga cggctggtcg gtgctgggct tcgcaccctc ggggttgccc gggccggcgg
    25201 gcaaccgcga gactttcgtc tggctcggcg agccgggccg cgacggcggc gctcccgcgc
    25261 gcccggacgg ggtggacgtg tgagcggcgc gccccggcgg atcacgctgt tctcgcacgg
    25321 gcggatcgac gacaccggcc cggcggtgca gcggctcgtc gacgccgcgg cggctgccgg
    25381 ggtcgaggtg ctcatccccc atggcgaggc cgagaaacac gacatcagcg agcgcgaggg
    25441 atgccggctc ggggtcgatc cgagcgagga cacggacgtg gcggtcgtgt tcggcggcga
    25501 cggcacgatc ctcaacgcgc tccgctcgtg cgcctcgagc gaggcaccgg tgttcgcgtt
    25561 caactacggc gcgatcgggt tcctctccac ggtcgagcgc gaggacctcg acgacgggat
    25621 cgcgcggctg ctcgagggcc gctacgaggt gctcgggatg cccgcgctgg cggtcgagct
    25681 cgacggccag cgccgggtgg gtgtcaacga catctccctg caccggcggc ccaacagccg
    25741 cgtggcgctg ctcggctact cggtgagggg cgaccagctc ggccaggtgc gctgcgacgg
    25801 cctcgtggct gccaccccgg tcggatccac cggctacaac ctcgcgaacg gcgggccgat
    25861 cctggcctgg ggcgtggagg gcttcgtcgt gtctttcatc gcgccgcaca cgctcaccgc
    25921 gcgagcgctc gtggtcgcgc ccgacgacgt gctcaccgtc cacaacctgt ccgacacgga
    25981 cccggtcgac gtggccaccg acaaccgcag cgtggccgag ctggcgccgg gggcggagat
    26041 cgaagtccac ttcgtccagg accaggtgcg cctcgcccag gtcgagggcg cgaacttcta
    26101 ccaccggatc cgcgcgaagt tcggccgcct ggcttactga tcgcagcggc ggccgcgggt
    26161 ctggcggcgc tcggcgccgg ctgtggctcg tcggaggctc cggccccgcc gccccggggg
    26221 ctcgaggtcg cgctccagga caacgccgtc ctgctcgagc gccactacta cgaccgcgag
    26281 cgggccttcg accggctgcg cgagctgggg gtgagctgga tccgcgtacc cctgatctgg
    26341 gaacgggtcg aacgcgaggg cttcggtcag gtggacgcgc tggtggacgc cgcgcgcggg
    26401 cgcggcttcc aggtgcagct caacctgatc ccacccgcgc ccgcctcggc gaccggtgac
    26461 ggccggccgg gcgtgttccg ccccgacccc gcggcgttcg gagcgttcgc gcggcgcgcg
    26521 gccgagcact accgctcgcg cgtgcgccgc tggtcgatct ggaacgagcc gaacctcgtg
    26581 aactggctgc ggccggtggc cgagatgccg gagatgtacc ggcggctcta cgaggcgggg
    26641 tacgcggcgg tcaagggggt gagcgcggat gcgcaggtcc tgatcggcga gacggcgccg
    26701 tacgtgcggc gcggggacgg catggcgccg ctgtggttcc tgcggcgcat ggtggccggc
    26761 gggccgctgg tcgccgacgg ctacgcgcac cactcctacg acttcgaggc gccgcccgag
    26821 cgcgactacc cgggcgacga caacgtcacg ctggggtcga tcgagcggct gatcggcgca
    26881 ctcgaccggc tggccgcgtc gggcgcgctg cgcgacggcc acggccgagc tctggacgtc
    26941 tggatcaccg agttcggcta cttccagacg accgagcgcg cactgccgcc ggcgcgccga
    27001 gccgactacg tgcggcgggc gttcgacctc gccgcctcgc actacccgcg cgtgcgccag
    27061 ctcctccagt acctgctggt ctcaccgccg gagggcttcc ccggcggccg cttcgacacg
    27121 agcctgctga cgcacgcggg ccgctcgacc ccggcgtacg gggcgctggc ggcatggtcg
    27181 cgcgcggcgg tgcgggcgcg gcgggcgctg ccgccgccgg gcgattgacc ctcccgccca
    27241 cgagcactag cgccgtttgg acatacaaag gcttattttc ggactcattc ttccatataa
    27301 tggacacatg actcaactgg ccctcctagc gcaggaggcg ggcgttcatg aacgtacgct
    27361 gcgacgagcg gtgaaccaag gaaccctccg cgctcatcgt cgaagcccga ggacgctcga
    27421 actgccgctc tcggagcgtc tatacgtgcg ccggtcctgg ccgttgatcg ccgccctgcg
    27481 cagcgccctc aggaccgacc cgaacgttcg ctttgccctc cttttcggct cgacggcgcg
    27541 ggggaccgac acgcctgcaa gcgacgtaga cgtgctggtt gaactgctcg acccaggcct
    27601 tgagcgcgtc atcgacctcg gagaacggct cacggcggct agcagtcgcc cggtggaaat
    27661 cgttcgattg gaagctgtcc gggacgacgc gtcgttcttg ggcgatgtgt tggcggaggg
    27721 gcgcgtgctc gtcgatcgag acgaggtctg gccgtccgtg cgttcgcgcg agcgaatgct
    27781 gcgcgggcgg cgacgccagg aggaagtccg tcgtgttgag gcggcgctag ccggggtcga
    27841 tcagcttctc gccggatgac cgatggcttc cgcaccgcgt agggctgacc gcgggcgctg
    27901 gcggcgcgac atcctgcggc atctggagga tttccctcgc cagtacgccg cactcgagac
    27961 ggcgatggca gcgttcggcg gtgacttcga cctgaagctg ttcaagcagg cattcgaaac
    28021 gcatgaggat ctggaggcgt acaacagggt gcagtcggtc gaacgggcgc tcggacgcgt
    28081 gcagaatttc gtagccgagc ttgccgacgc cggcgtcact ctggcccggc taccacgccc
    28141 gccgatgacg aaggacggct gtcaagctca gcaggcgttc gaggctctgc gcgatgccaa
    28201 ggtcatgggc ggcagcctgt gccggcagtt gacgcgtgcc cagagggcac gttcgcgaat
    28261 cgagcacggt tacgtcgaga cccctgcagg cgatgtccat cgtgcggcgg agctcgtgca
    28321 cgagtcggcg ctcggcttca tcgccgcgta taggccgtgg atcgcggagt acctgtagcg
    28381 gggctgggcc gcccgctccg tcggcccccc ctggtacacc tgctggcccg atgctgctcg
    28441 agctgcgcgt agagaacctc ctcctgatcg accgggccga gctgcgcttc gggcccggcc
    28501 tgaacgccat cacgggcgag accggtgccg gcaagacggt gctcgcgcac gcgctcgacc
    28561 tgctgctggg cggcaaggcg cgtgggggca tcgtccggcc cggcgcgtcg gaggcgtacg
    28621 tggagggcgt gttcgagctg aggccgggcc tgctcgacgg cgacgagctc gccgacctgc
    28681 gcgagcgggt ccccgacgac gctgacgaga tcgtgctggg ccgccgggtg ggcgccgagg
    28741 ggcgcacccg cgcgttcgtg cagggccgct cggccacggc cgccgacctg cgcgagctcg
    28801 gcgggcggct cctgtcgttc tacggccagc acgagcaccg gcggctgact gtcggttcgg
    28861 cccagctcga gatactcgac gcgttctgcg gcgaggccca tctggcgctg cgcgagcgct
    28921 acgccgaacg tcaccgtcgc gcgggcgatc tgcgccgcga gctcgacgag ctccgcgccc
    28981 gcaccggcgc gcgcgaccgc gatcgcgacc tcctggcgtt cgagctggcc gagatcgacg
    29041 agctcggccc ggacccgggg gaggaggagg gcctcatggc ggagcgcggg cggctgcgcg
    29101 aggtcgacgg cctgcgtatg gccgcgggag ccggcgccga ggcgctcgcc cccgaggatc
    29161 ccgaggctcc gggcgcggag gccgcgctgg ccgacgcgga gcggctcgcc gacggcgtgg
    29221 cgggcgcgga cgcggagctc gacgcgctcg ccgagcggct gcgcgcgctg cggatcgagg
    29281 cggaggagct gggcggcgac ctgcgccgct acgcggacgg cctcgaggcc gacccggcgc
    29341 ggctcgagga ggtggaggcg cggctcgact cctacgatcg gctcaagcgc aagcacgggg
    29401 ggtcggtgga ggccgtgctg gagcacgccg accgctgccg gaccgagctc gcgcggctcg
    29461 actcggcgga ggaggactct gagcgcgccc gggccgagct ggcggcggcg gagagcgagg
    29521 agcgcgccct cgcggcgaag ctcacgaagg cgcgcaccgc ggcggcgccc aagcttgcga
    29581 aggcggtgct ggccgagctg cgccagctcg cgatggagga cgcgtcgttc gaggtggtgg
    29641 tcgagccgcg tggggccgcc tcgcggggcg cggcagcctc gcccggcggg gcggctccgc
    29701 ccgccgccga cctcggcccc accggtgccg accgcgtcga gctgcgcgtc gcccccaacc
    29761 cgggcgtcgc gcccggcccc gtccgcgaga tcgcctccgg gggcgagctc tcgcgcgtga
    29821 tgctggcgct gatgagcgtc gccaacagcg gcggcgacgc gacgctggtg ttcgacgagg
    29881 tcgacgccgg cgtcgggggc cagaccgccc gcgcggtggg cgagcgcctg cgcgggctcg
    29941 cggccggccg gcaggtgctc tgcatcaccc acctgccgca gatcgcgtcg atggccgact
    30001 cgcacttccg catcgagaag gacacgggcg gcgacgtggc gcgcaccacg gtcgagccgc
    30061 tcgagggccc ggaggtcgtg gccgagctgt gccggatgct cggcgccgac acgtccgacg
    30121 ccggcgcgcg ccgccacgcc gaagagctgc tcgccaccgc ggcgtgagcc gtcgcggctc
    30181 ggcgtcctcg gtcgcttgcc ggccggtgct cgccccgtag actcaccccc cacggagggg
    30241 aaccgcctcg cacgcatggc cgcgccgcgc atcagaccac gctcgctccg accgcgcccg
    30301 ccggccgcgc tcgccaacgg gcgccccgag cccgcgaccg gcttcaccgg cccggcccgt
    30361 ctcggcacgc gcacgaagcg gctcgtgaag cggatcgggc cggaagacat cgcgatcatc
    30421 gaccacgccg ggatcgaccg cgtctcgggc gaggacctcg tcgcctgcgg ggtgaggcac
    30481 gtgatcaacg tcgccacctc cgccgccccg cactacgcga accgcggccc ctcgatcctc
    30541 gtggacggcg gcgtgcacct cgtcgacatg ccgggcgccc cgctcttcga ggtcctcaag
    30601 gacggccaga cggtcacgct cgacggcggg aagctgctgc gcgacggcga ggtcgtcggc
    30661 gagggccagg tgctcgaccg cgagacggtc ctccgcgcct acgagctggc cgagcgccgc
    30721 atcggcgagg cgctcgaggc gttcgccgag aacaccatga cccacatccg ggaggagcgc
    30781 gagctgcttt ccggcgagat agcgctgccg gacttcgaca cgacgttccg ggaccgtccc
    30841 gcgctggtgg tcgtgcgcgg tgtggaccac aagcgcgacc tgcgcatcct gcgtccctac
    30901 gtgcgtgaga cgaacccgat catcgtcgcg gtcgacggtg gcgccaacgc gatcctcgag
    30961 gagggcctcc agcccgacat gatcgtcggc gacatggact ccgcgaccga tgacgcgctc
    31021 cgctcgggcg ccgagcttgt ggtgcacgcc taccccgacg gccgcgcgcc cgggcgcaag
    31081 cgcctcgacc cgatggggct cgagtacaag gtcgtccccg ccccggccac gagcgaggac
    31141 gtggcgctcc tgatcgcttc ggagaagggc gcgaacctaa tcgtgagcgt gggctcgcac
    31201 ttcaacctcg tcgagttcct cgacaagaac cgcgcgggca tgtcctccac gttcctcacg
    31261 cggctgcgcg tgggggagat cctggtcgac gcgaaggggg tgagccggct ctaccggccg
    31321 tccgccggcg gccgcccgat cctgctcgtg gccgccgccg cgctggccgt cctgatcgtg
    31381 atcgtcgcga cctccgagac cctcgggccc ctgctcgacc tcctctggct caagctacaa
    31441 gtgctcctgg gcctggagta gcgcggcgtg ctcgactttc gctaccacgc gctctcgctc
    31501 gtggcggttt tcctggccct cggcatcggg atcctgctcg gcacgacgat cggcgaccag
    31561 ctcgtgtccg aggccaaccg cgacctgtcg tcgtcgctgc gcggcgacgt ggtcgaggcg
    31621 cgccgggagt cgcgtgccgc gcgcgacgcg ctcgagaacc gcgaggcgtt catcggcgcg
    31681 tcgttcgacc acatcgcggg cggcggcctg cgcgggcgca acatcgcgat cgtctcgtcg
    31741 ggcgagctgc cgggaagcgt cgagagcggc gtgcgcgacg cggtccagga cgccgacggc
    31801 gggatagcga gcgtgtccga gctggtcgcc ccgccggagc cggccgttct ggccgaggtc
    31861 gccgggcggg gcttcgacga cctgcgcccc ggcgatccgc gcatggccgc gctcgggcgc
    31921 cgcatcggcc gcgcgctcgc gaacggcggc gcgctggcga gccgcctcgc cgaccgcttc
    31981 cccgagcgct tccgcggcga cttcccgcgc gtggacgcgg tcgcctacta ccgggatccc
    32041 ggcgccgagc gctccgacga cgaggagcgc ttcgagcagg gcctgatcga cggcctgcgc
    32101 gccggtcgcg acccggtcgt gggcgtcgag gagctcgaca ccgagccgag ccagatcggc
    32161 ttctacgagg accggatgca ggccagcgtg gactcggtgg acagcccggg cggccggatc
    32221 gcgctcgtgc tcacgctgcg cggcgcgcgc ggcagcttcg gctacaagga ctccgcggac
    32281 gccccgctcc cggagcccga cggccgctga gcgcgtccgc ccctcccggt accgtcgccg
    32341 gatgcatgct ctgccgctcg cgatctcgct cctgaccgcg gcggtggtgg cgccgccgct
    32401 cgtgcgcggc ctgcgtgagc agggcttcgt gcgcgagaac tatcgcggcg agaaggtcgc
    32461 cttcccggcc ggaatcgcgg tgcccgtggt cgcgctgctg gcgctggtac cgctctcgct
    32521 gatcgccgag ctcgcggacg aggaggtgtt ccggcccggc acccaggccg ccgtcacgta
    32581 cgtgatcggc gtggccctgc tcggcctgct cgacgacctc gtggggagcg acgctcccgc
    32641 ccgcggctgg cgcggccacg cccgcgcggc ggcaaccggg cggctctcca ccggggccat
    32701 caaggcggtc ggctcgctcg gcctcgcgct ctacgcgctg tcgggcgcgg gccgcgacac
    32761 gggggagtac ctgctcgcgg tggtcgtgct ggtgctagcc accaacctct tcaacctcct
    32821 cgacctgcgg cccgggcggt cggcgaaggc gctcgtgcta ctcggcctgg cgctcagcct
    32881 cggcgccatg gacgccgacc cgctctggac ggtcgggctc ttcctcgggc cgatcctcgt
    32941 gctcctgccg ctcgacctgc gcgaggtcgg gatgctcggg gacaccggct cgaacgcgat
    33001 cggagcggtg gccgggctgt ggctcgttct cacgctgtcg gtcaccggac aggcgatcgc
    33061 gctcgccgtc atggcggcgc tcacgattta tggggagttt cggtcgatct ccgccgtgat
    33121 cgaccggacc cccgggttac gtcatctaga ctcggtcgga aggacctcgc atgcctaact
    33181 cccgctcgca gacaaggttc atcttcgtca ccgggggagt ggtgtcggcc ctggggaagg
    33241 ggatcgcggc agcttcgata ggccgcctgc tcgtcgcgcg cggcttcagc gtcacgctcc
    33301 agaagttcga tccgtacatc aacgtcgacc ccgggacgat gagcccgttc cagcacggcg
    33361 aggtgttcgt caccgaggac ggggccgaga ccgacctcga cctcggccac tacgagcgct
    33421 tcaccgacgc gaacaccacg cgtgggtcga acgtcacgac gggcgcgatc tacaaccagg
    33481 tgatccggcg cgagcgccgc ggcgactacc tcggcggcac cgtccaggtg atcccgcaca
    33541 tcaccgacga gatcaagaac cgcatccggc tggtcgccga ggccgcggag gtcgacttcg
    33601 tgatcaccga gatcggcggc acggtcggcg acatcgagtc gctgccgttc ctcgaggcgc
    33661 tgcgccagtt ccaggtcgac cagggccgcg accgctcgat gttcgtgcac ctcacgctgg
    33721 tcccgttcct cggccacgcc ggggagctca agacaaagcc cacccagcac tcggtgcagg
    33781 agctgcgccg catcggcatc cagccgaaca tgctgctctg ccgctccgag ggcgccctct
    33841 cgcgcgagat ccgcgagaag atcgcgctgt tcgcgaacct gcccgttgac gcggtgatct
    33901 ccgcccgcga cgtcgactcg atctacaagg tcccgctcta cttccgcgcc gagggactcg
    33961 acgaccagat cctcgaccac ttccggatgg aggcgccgcc ggccgacctc gccgactggg
    34021 aggcgctcgt caagcggggc gactccgccg aggagaccgt ccggatcggc atggtcggca
    34081 agtacatcca gctcgaggac gcctacctgt cggtgatcga ggcgctcggg cacgcggggc
    34141 tgcacaacgg cgcgcgcgtg gagatccgct gggtcgacgc cgagggcgcc gagtccgggg
    34201 aggccgagcg ccagctcgag gagtgcgacg ggatcctgat ccccggcggc ttcggggtgc
    34261 gcggggtgga gggcaagatc cgcgcggcgc ggttcgcgcg cgagcgcggg gtgccgtacc
    34321 tggggatctg cctgggcatg caggtggccg ttgccgagtt cgcgcgccac gtggcggggc
    34381 tcgacggcgc gaactcgaca gagttcgacc cggagacgcc gttcccggtg atcgacctgc
    34441 tccctgagca gaaggacgtg cgcgacatgg gcggcacgat gcgcctcggc gccgacccgg
    34501 tgaagctgca cgagggcacg gccgcgcgct cgatctacgc ggagcccgtg gtctacgagc
    34561 gccaccgcca ccgctacgag gtcaacaact tcctgcgaaa gcgcctcgag cacgccgggc
    34621 tggtctgctc cggcacctcg ccggacgacc gcctggtcga gatcatcgag ctgcccgacc
    34681 atccgttctt cgtcgcgtcg cagttccatc ccgagttcaa gtcgcgcccg ctgcgcccgc
    34741 agcccctgtt ccgcgacttc gtggcggcgt cgctcgagcg ctcgcgcgag ggcagctcga
    34801 agggcgaggg ccgtttcgcg cgcgacggcg aggcggccac cgagccgggc gccccgcaga
    34861 cgagccgcac ggcactgacg gattgatcga gggcgcgccg cccgcgcggg ctccgcgcgc
    34921 caccgccgcc gagcgcgcgc ggctgcgcga cgacttcgtt cgcctctgcg
//
LOCUS       DASVPL010000101        45829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_129104, whole genome shotgun sequence.
ACCESSION   DASVPL010000101 DASVPL010000000
VERSION     DASVPL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 45829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45829
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129104"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="VF517_04795"
     CDS             <1..1899
                     /locus_tag="VF517_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8052289.1"
                     /translation="TAMVALALPALALAHIERPSYWPDPAPDTSVTPPAGGKVPKARP
                     LSSALVDRLPGETRVVCQKKSLTYLKRSIAKARKDGYTYRPSEHLKLSAKRAERLMII
                     NRRLFKRCKYKEIQPAVTASTNNDRVVVMPGIYTEPTARKAKTFDPACDKWEIKTEFG
                     DPGALAYDYHLNCPNDQQLIAVLGRGPNTAPPPEPPRYDRHGIPNIGPCIRCNFQIEG
                     SGVSADDTIVDAGRVESGNGAPIDAKKDVGIRADRADGFVLRMMTFRHAREHGIYVME
                     SDGYLLDRFKAFYNGLYGTLTFVEDHGIQQHCETAGHADSGIYPGAPVDTGEQRPEGT
                     EFRYNQQIRFCDSYHNLAGYSATSGNAVHVHDNNFYGNALGLNTDVATAAGHPGYPGD
                     SMLYEHNNIYSNNFNPYEEGSDVIPAFPYPVGTGLWIAGGNNHTVRNNNIYDNWRRGS
                     MVFSIPDVLICGEQTDNHNQKGCDESRTSTSYRNKHYSNVMGRGPDGKPAPNGTDFWW
                     DEFPGNTDNCWYLNEGPAPITYSPEPLPNCNDGKNSEQSVGHGNLQNESELASCLAAF
                     ETRNFDQETTSCPWLFTPPKPGSSGALAQDSDAGQARQLELFLRYCAENPQSNTCKAY
                     LSPLGLKR"
     gene            1917..2270
                     /locus_tag="VF517_04800"
     CDS             1917..2270
                     /locus_tag="VF517_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052290.1"
                     /translation="MLLACALALGACGDSSDSDSKPGGPAPRPANLPPDFNIQLFNCA
                     NWQQSDDKIRAYVVDRLQQIVGGQITGQGADGRGSTITDEQATRLFDGTCGSPQARGF
                     LLYKLYGHAAGFGGG"
     gene            complement(2275..3600)
                     /locus_tag="VF517_04805"
     CDS             complement(2275..3600)
                     /locus_tag="VF517_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8052291.1"
                     /translation="MALPPGPRVPGVLQTAAFMRRPIDFLLRCQARYGDCFRARFQGI
                     GEVVYVADPGEVERVFKGPADVFHAGEGNAALFEPAIGRLSSMTLDEGEHLRRRKLLL
                     PPFHGEALRRWSDVFAEVTEREIERWPVGEAFELLPPMKRVTMEAILRAVMGVRDPAR
                     LEELAAGILRLDRLSGVVLPLPPLQRDLGRWSPWRRFVAARDAMDELVYREIAERRAA
                     PDPAGDVVSLLIESRYEDGGAMTDQELRDEMYALLAAGYETSSAGLTWCFERILRTPR
                     VLDRLLAEPEDDDYLDAVVKESLRVRPPATDATRILKSEAEVAGYTLPAGTQVVIALP
                     LLHLRPNTWSDPHEFRPERWLEGEGEPYTFIPFGGGVRRCIGAAFAHLEMKVVVRTVL
                     DRARLRAASPRLEAQRLHHVVVVPTRGGRVVMDERLAPRAGERAAALAA"
     gene            3631..4575
                     /locus_tag="VF517_04810"
     CDS             3631..4575
                     /locus_tag="VF517_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8052292.1"
                     /translation="MTAAATRAGLALGALAGALLVNRELAGREAKPAEARAGLLVPTA
                     LGELHVLEEGDPSAPVAVLLHGFGGSLHWFDRLAPLLATDHRVIRIDALGHGGSAKPR
                     IDYSFEQHAAAVAEALGRLGVEHALFVGHSFGGALAIAVAERRRELVERIVLMDDGPD
                     NDFGDQPLMTTMGFVPVIGELLRRLAFDSAIRDGFRDAFADGFDLAGGFDDPDQVVHD
                     YRAMTFTSYRGSWDAEEAYMDATPLDERLRRLGMPSLVIFGEHDRFFRAEEAAAAYRR
                     VPGLRVEVIAGSGHSPNVERPEEVARLVRELVAGAAAG"
     gene            4572..5378
                     /locus_tag="VF517_04815"
     CDS             4572..5378
                     /locus_tag="VF517_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8052293.1"
                     /translation="MIGRRPDLGRRFGGTHAVVTGGSAGIGLAIARRLVALGGSVTLV
                     ARDEERLGRAAASLGERARTLALDISDADAVADRLPAELAEWPADLVVNCAGISTVGE
                     FMDLDGPALRREMDVNYFGAVWVTRAVLPHFLERGRGHVAIVGSTASVIGIHGYGAYT
                     PPKFALYGLAEVLRAELAPKGIGVTIVLPTSTRTGMLERELEEAPPETRRLITAERIL
                     EPEEVAEAALRAVASGRFEAIPGRDVTLQTRAYRLAPSVGRRIVDRKARG"
     gene            5375..6616
                     /locus_tag="VF517_04820"
     CDS             5375..6616
                     /locus_tag="VF517_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8052294.1"
                     /translation="MSDGGAPGVAAESAPSASPRAAPGEAGARELIETLARLERRPCS
                     PGEREAAEWIAARLERAGCERVDLEEEVAYGLYAPTLTALGLLGALASLLCIAGRRVA
                     ASAISIAGLVTVVDEAQNGPRLVRRALYPARTTTNVVARIGPDDAPRTVVVLAHHDAH
                     QTGRLYDQSLLMAAHRHAPGLLARMKTSLPQWWVGCSGAVLTLVAVATRRRRHAAAGA
                     VLNLVGAAAMGEVARNPVSPGANDNLSGVAGLVTLAELLRERPLPGLRVLLVSCGAEE
                     ALQEGIRAFMARHGNELDPARTAFVNLETVGSPRLIMLEGEGPIWMEDYAEPSFRDLV
                     ARCAEAEGIELERGFRARASTDSVIPSRAGHPTATITSITAWGALANYHLPTDTAANV
                     DHDSVQRAARLAYATAAAFRA"
     gene            6681..8033
                     /locus_tag="VF517_04825"
     CDS             6681..8033
                     /locus_tag="VF517_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8052295.1"
                     /translation="MATETRDAPAATRRARLGPLLGDLQALRQDPLGLFARGAASGAD
                     VVRFRAAHRNVYLLVGPGAIAHVGIHNRDNYFKGVSYDALRVPLPNALLTIDGDEARE
                     RRKLLMPLFTRRSLLPDVSTITAAVDDLCRRWDEFARTGTEFDVHSEMNRLTFDVVGR
                     ILVGAELGDEMGRLEASIARASEWVARRTRALVPLPTAIPTPGNIGYRRNYRALAAWA
                     DGLVASSRGATGDGVAGRLVAASRDGAGMDDRQLRDEVAAFLMAGHQTTASALSWTWY
                     LLARHSGEQERLAEACEFALAGGAPDMERLDVVPYLRQVLDEAMRLYPPGWAFTRTPK
                     ADDEIAGERVPAGSIVIVCSYANQRSPRFWDDPETFDPGRFATGRRESIGPYSYFPFG
                     VGPHACIGKHLAEIESRLALALLARRYRVEAVSGAPVAPEPAITLTPRTPIRVRVTRR
                     "
     gene            complement(8030..9298)
                     /locus_tag="VF517_04830"
     CDS             complement(8030..9298)
                     /locus_tag="VF517_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052296.1"
                     /translation="MARLTPRALPGALGALMLAALIAVAMALPDASVGQGRDATTTLF
                     SRTPDGNTPNGPSTNPHISADLRYSQIVAYESEASDLVARDTNGLKDVFAVRRAGSFG
                     DTGSAWTPGKTQLVSRSATGKPANGPSFDATTDGSAKDRARCVAFLSDASNLVSGDTN
                     GQTDAFLAKAPKFVPTRVSLPKNKQSSSDTTHVAVSGDCSKVAFVSGGGLYVRSGKST
                     KRIDVKGAEADPSYDTGDTTGLVFGASGGVYLLAEGASKPTLVAKGGRNPGFIQRRRQ
                     GKVLRTVIYETDRGGFSQIGLRSIGGGERIVSSWDGDVGNGDSREPSIFNAGFNAAFV
                     SDASNLPIKSSGALGDRNGLRDAYFWSGSPNVPQPVTILESVDSGNDPLSSGAEHVST
                     SYYRNYVVFDSAANRTGAEPQIWMRYLGGI"
     gene            9394..10284
                     /locus_tag="VF517_04835"
     CDS             9394..10284
                     /locus_tag="VF517_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052297.1"
                     /translation="MSDEESSFEYKRVGQHLIRRPRAVERWQVLVDVRGSARWITCSA
                     RLSRRFERAASSEELNADRAAGRMVRACVANRPVTDPASPNPSVRTRLRVRALTWRLS
                     PPLAFGGPRRVGTAAARAAQELAERDLRRHRGSAERIEGEAQAGWAQNRERLAGVEQR
                     AAYFLGGAGLGAGVLLASTSLLVDSDARPEGVARIVAAIGLLITILGLVASAAYALAA
                     TMQTFGRITPDITSQVIERAKRPDEQARMERAARLIASRRRSSYIANWKVARLTRAAI
                     ASACAVLGLLIMLTAVLVTI"
     gene            complement(10312..12036)
                     /locus_tag="VF517_04840"
     CDS             complement(10312..12036)
                     /locus_tag="VF517_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052298.1"
                     /translation="MTPRHGILAAIATTAIALLCAAAATAAPSFRPPAVAGPTASALP
                     SVAFDPAGNAAIAYLDGQEVKVASRPPGGGWGAPQTVGDVGLGDSHAFAGFDGAGNLT
                     VIWATSANETAYAVTRAAGTTTFGSPQQIATGINPGLQMDVDFARSGAILLVWGRGFG
                     SGVRAVRRPAGGQFEPEETIGSGGDSPRGAVGEDGTMALVWHQQGSPAQIAVRPPGSS
                     SWEGPTDLGAPVGPLSQPNVAVDASGNTLAIWIDEQRRINARVRPAGAGAAFGAADQV
                     ADLPGDAYGTEFFSPPQLAFDGAGNATLLWSQINNGGDAGSPWRLFSAFRRPGQAFEY
                     PVAVSSEGENAEYGRLAVAPDGTALATWRNGPHGHDYRQQNTGAILAVHGASGAWSTA
                     PLTLSPSDSYQPAMATASSGAGAVAWIRMHDAQCRQLEVSDFAEGAPAALNPPPRSCD
                     PPPPPDLEPPIAALGGKTKQRAHTTRRIDLTIVCDEACSGQAKGTLTLKPRRGKSSKV
                     KLAKAGYLAKAGSKATVRFRLTKAQARRLAGHLAAGGSATLGVTTAARDLAGNEGPPL
                     RRTVRLVR"
     gene            12143..12517
                     /locus_tag="VF517_04845"
     CDS             12143..12517
                     /locus_tag="VF517_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052299.1"
                     /translation="MKTLIPLTALVALLVGAAPSPGKDTATRVSRSGDCSGASNWKLK
                     AKPDDGRLEVEFEVDQNRNGVEWKVKLRRNDKTLVSTTRRTQAPSGSFSLERRIGDPA
                     GKDRISAVATRDGETCRGSLSI"
     gene            complement(12528..14759)
                     /locus_tag="VF517_04850"
     CDS             complement(12528..14759)
                     /locus_tag="VF517_04850"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HEX8052300.1"
                     /translation="MVTAAHAPLHEDLAAVASAVSASPGTDPVPTLASLLYRDHYVRP
                     HGRAPITVLDAVTDRDFQESLIRAARPGVTWEPGWRRAGAEGDRVTVVAPHGVRFWVA
                     PERVRGVADGDPDGCAVLVPTVRRALAPGYFAVIGREAWDAPDEPAGMVRLYWHVREQ
                     AAAALLATVQSALDGARVPFKLKVLRSPAAYRRADAAVLYVPRHRFEEARGPLRGVYA
                     ELAGRLRPSVPLFTKRLAPGLALAEDPGLGESFGQHRCRLVARALWRAHEDGHRDAAS
                     KRAAIAAAFAEAGLDPARPHLAPGSEDGYEPLPGKAAPVAEHRDSPPSDDADPAELLA
                     AAGRLGQTIVADALWDRRGEACGWIGRSSFGRPGSVALFVPRSTGLGPGLYDGTSGVA
                     LFLAELFARTGEPEFARTARGAVAFALARARGERPSSTSLGLYSGVPGTLLAAWRVAR
                     LLGDEPPDGGDADLLAGSLSDHCADARDDLLVGRAGEVLALLALARETGRAEYVAHAE
                     RIGRRLGRGAVTYAGHDSPAAGAEPPLTGISHGAAGVGVALLELAVAGDYPEALDAAH
                     AAFEYEQGLFDADAGNWPDLRTDELHPPGERRYAVAWCHGAPGVALSRLRARELDPAR
                     RAWYDEHVTAGLDTTRAELERRASLDVWDLTPCHGVAGLVEALWIGGHVLGRPDFTSA
                     ARRAAGAIARLPEAEMRSGTPCGGPSPALMLGTAGVGYQLLRLADAGAVPSVLCGLWH
                     APV"
     gene            complement(14753..15820)
                     /locus_tag="VF517_04855"
     CDS             complement(14753..15820)
                     /locus_tag="VF517_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8052301.1"
                     /translation="MSEAALVRRLVRAGVLASDDAILPDTAVERCPRRNRNTRVALSG
                     GAGVFVKQADPRAQGSRETVIAEGAVYHAHRGGGPLSAVLPGLVHFDPSEPLLVTELL
                     VGYGDLRSVCQAGGPAEFPIAAFRAAAAALAQVHTVTVGADGRPDGPEATPLGWVPAV
                     RRPDPAALETLSNGAMTGLEVVQRAMAIDAGLARAADVWRSESLIHGDVRADNVMVRS
                     HADGGIDVRLVDWELARLGDPAWDLAGLLEAAVTLALGRGETWGGDDPAAELAVPVIQ
                     ATTRAVWRAYIGAGGEGSSGEGELRARATDYCAARLVQTTLELLAGADELSGSALIAL
                     QVADNVFAEPERAAAELLALW"
     gene            15874..16107
                     /locus_tag="VF517_04860"
     CDS             15874..16107
                     /locus_tag="VF517_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052302.1"
                     /translation="MKMTPLVGGGHGTPALMHQSLPQPLDCGRHIPEQADGSPVSGRT
                     ASSREKFARKLSRRAPVTKDALPEMSRVRTSLS"
     gene            17405..19552
                     /locus_tag="VF517_04865"
     CDS             17405..19552
                     /locus_tag="VF517_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HEX8052303.1"
                     /translation="MHLKTFRIRNFRRLRDVRIDLDTATTIFVGANNSGKTAATHVFR
                     TFLRKSPFRIYDFSASTWQLFDEFDVEAGDPDAELPRITLDLWFEVEDANLQRVINLL
                     PSLDWNNEPVGLRIEYAPVDGRELVGRYQQVLNERPPDPDVPTASWQPWPQSLTDYLS
                     KRLTDEYELRYLVLDAKSCDEEFRFPSDYEPLRLPTGQSGARLVDSLIRVDFLDAQRH
                     LTDTEAHGRAEELSKRLARFYERNLEQHDQDVAALSAVAETEARLNAHFAEVFEPTLE
                     RLQRLGYPGFADPELVVRTALDGQTVLNSSAQVFYKLSEGLKGGEGARVLLPDQYNGL
                     GFKNLIYMVVEVLDSHFAWHAADDVLPPVHLVMIEEPESHLHAQLQQVFIRKIREVLP
                     ESEEGLETQMVVTTHSAHVLYESSFQHIRYFRRSVPPADTRESDVRNLSLFYDREEAP
                     TRRFLLQYMKLTHGDLFFADGAVLVEGNVERLLVPLIIEKDVRELRSRRLAIIEVGGA
                     HAHRFKQLIEFLDLPTLLIADLDSVIPNEQTGQSRSCMTTEADATTANPILKDWLPQV
                     ELIAELLTLDATRKISMAESNGGGQVRVAYQTTETADWSGESKMLAGRTFEEAFALQN
                     LNWIQSSNQVALGLGVKDAAQKTLDDLHEAIYNKVRTFDKTAFALALIDADENAWVAP
                     AYIVEGLEWMRGVLVDPTSGSEDDGQPDAAVTA"
     gene            19549..21426
                     /locus_tag="VF517_04870"
     CDS             19549..21426
                     /locus_tag="VF517_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX8052304.1"
                     /translation="MTGRAGAPDTDADVEIRALLDETPRRAFVVIAGAGSGKTTSLVK
                     ALAHVARRDGPALRNRGQKVACITYTEIAASEIAEDVGAGPLVHVSTIHSFLWTLVRP
                     FQKEVIAWVADKIDRKIAEAEAKLQSERARESTKERAGTDVERYTRHKEALLNVEQFR
                     YGIGADYGGGVLGHGDILQLVPTMMVERPLFARTVAARFPYVFVDESQDTTSAVVDGL
                     KAVAKAADGKMSLGFFGDPMQRIYPEGIGDIELDNGWARVEKPENFRSSERVLRVVNA
                     VRADADGLQQVSGLPQEQRRKGEAWFFVLPADDHRQGSLARATEWLTKNSNAGDWSAE
                     SARGAKVLMILHEMAARRLGFHSLWAAFHQAGPGSSLDRAFDEGAAWPLRPFQQAILP
                     IVSQVESGGHTVAAIRAHAPGLSEEALAETDPRHALSAIRDGISALRELMETGGPGSV
                     AQALRVAAESGLVDLDPRLSAHLELPGGPGAGGEIEEADQPVLDAFMQCDVRQLDAYF
                     DYVATRTPFSTQHGTKGAEYDRVLLVLDDDEGSKFTLYSYEKLLGLKPLSSADQKNHN
                     EGKDSVIERTRRLLYVSVSRAKEALAVVLFASDVDAAAEALERSGLPGDAQVLKLPDI
                     S"
     gene            complement(21657..22349)
                     /locus_tag="VF517_04875"
     CDS             complement(21657..22349)
                     /locus_tag="VF517_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052305.1"
                     /translation="MVAAVDHLVFWANVVAPQTVYDGMTVSNPPRAYYTLARSGLECA
                     AQAVWVLDHDASPERVDRHLRLLYHDLRQMALAFEAVGDDRSTVARERMTVLAERLGD
                     AERLARIKKHEPKYSSMVRETADAIGLTPGDAEFLWRSTSAAAHGKNWFQHVGYTASV
                     GEQYEPGYFRAALQPDPMEITRSVTAAANLTLHGVLRFATRSGHDPSPLVEAAFSKLK
                     RDTPLKDPRPDQ"
     gene            complement(23381..24322)
                     /locus_tag="VF517_04880"
     CDS             complement(23381..24322)
                     /locus_tag="VF517_04880"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX8052306.1"
                     /translation="MEFFVGSLAYEVTASDLAAFVGKVVEVNAAMVQTDRETGRSRGF
                     GAIDVPDGALIDAHNEPVEGFGEGAEQEARERMALLNGQILLGRRPIVSRSRVAAASE
                     PFLSGVRGALADDGQHVIPNEEQFRDVLDAALGRAQAEEVLTPSARDRVQSSVGSVTF
                     IGTRVLDYLSDHRSELHELVNPRQFEELCHELLRRTGFYDLQLTQQSRDGGVDIYGTR
                     VDRGQRNFYVVECKHWDPSNRVGSPIVQQTFGVMGLTQADKAMVVTSSYFTGPAIGWQ
                     AVSHERLILRNRDVLGAWIRAVRRGEPECMLWLPGDP"
     gene            24547..25020
                     /locus_tag="VF517_04885"
     CDS             24547..25020
                     /locus_tag="VF517_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052307.1"
                     /translation="MEIELGALTYLFDTAPTTVGCGDDRVVAVWGRSRPADTPRDASR
                     LKGFLLDPAIWSQAGRGLGHFVGHAAGGNMDMNLFPQAIGLNRGTSKRGRVWRSMERY
                     VARHPGTPLFVRPTYGDQTWIPVMIDYGLLVDGTLWSDRFENHDWSESTGIPYDC"
     gene            complement(25331..25798)
                     /locus_tag="VF517_04890"
     CDS             complement(25331..25798)
                     /locus_tag="VF517_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052308.1"
                     /translation="MRRGDSWSLNITNVFMAVPFDDVYAKFYRANICGPIIEAGLTPI
                     RADEMPARAEFEHIMDRIMEGIARSRFVIADVTGWNTNVIYEIGLAVGISKPILLLCH
                     KRHFQDKDIPFDFRSYPLIGYDPYDAEDLQTRLTVKIGEMMDATPPPKGTSDD"
     gene            complement(25959..26031)
                     /locus_tag="VF517_04895"
     tRNA            complement(25959..26031)
                     /locus_tag="VF517_04895"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25996..25998),aa:Ile,seq:cat)
     gene            complement(26099..27034)
                     /locus_tag="VF517_04900"
     CDS             complement(26099..27034)
                     /locus_tag="VF517_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052309.1"
                     /translation="MALGACGGGGDDGGSPSGSDQPVGRAQPFPDPSSRTLDELRAGL
                     GPGPLMAKSVSVLQPGRNRFAFALFDRTRRQIADTPAAVYVAPEGGAVVEGPYPARYR
                     SLEVEEPHRSDSVKHDHDAAHSIYVADIPFKREGNYEVLGVTQLDQRVVGGTPVRVRV
                     ERPTAPPAVGDAAPRVNTPTRDEVRDLSEIDTREPPSTMHEDDLADVLGHRPVMLLFA
                     TPRLCRSRICGPVVDVLEQVKADHPDDAAYIHMEVWNGNSRDGGERPQVRAFGLPSEP
                     WLFAIDAQGRVAARIEGAFSAEEAEEALREATSGR"
     gene            27193..27921
                     /locus_tag="VF517_04905"
     CDS             27193..27921
                     /locus_tag="VF517_04905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052310.1"
                     /translation="MKGRAALITGGSSGIGLAIARALGQDGYSLTVSARRPEKLEEAV
                     EGLRGEGLEVECVPANMANEDEIKGLIARHRKRFGRMDVLINNAGVGIGGPIAEQQTK
                     HLDMQINVNLRGVYLTLRESIPLLKEAGAEHKKALVVNTASIAGKFGQAWLGTYSATK
                     HAVVGLSQAAHGELAESGIQVTAFCPGFVDTPMTGWVEGVPPEAMIQPADLGEAVRFL
                     LRTSPACIVPEIQFIRPGETQVKT"
     gene            complement(27922..28392)
                     /locus_tag="VF517_04910"
     CDS             complement(27922..28392)
                     /locus_tag="VF517_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052311.1"
                     /translation="MLESLDDERIIVGAYTDGDGGICPMLAAHRNGGRTNLASFAHAW
                     DAYTHATTMREASERELRTLRAMLEASLYYEDNAADLAEAAEEHRKLAGESAERERTD
                     TGERDRSDELRRRPGWSWLRVFRRLDEYEAAVAAAERQAAEAAERDERPERELV"
     gene            28587..29618
                     /locus_tag="VF517_04915"
     CDS             28587..29618
                     /locus_tag="VF517_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX8052312.1"
                     /translation="MRLGLQLGYWGLGISEQDQLKMVREAEAAGYESAWGAEAYGSDT
                     ATVLAWLAGQTEKIKLGAGIFQMPARSPAMTAMTAATLDNLSNGRMLLGIGSSGPVVA
                     EGWHGQPFAKQIQRTREYVEIVRKALTRERLEYDGETYKLPLPGAQGKPLKLMIAPIQ
                     ERIPIYLAAIGPNNTKLAGEIADGWLPFLFSPEHVDDGRRLLQEGADRVGRTIDDSFD
                     IAPNVNLCIDDDIEKARDMLRPILALYIGGMGSRERNFYNQAVQRYGFEEAAAKVQDL
                     YLDQKREEAMAALPAELIDTVALVGPKDRVRDRLAAYRDAGVGTMVITPLTEPENRAR
                     MLTDLAELV"
     gene            29633..30829
                     /locus_tag="VF517_04920"
     CDS             29633..30829
                     /locus_tag="VF517_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX8052313.1"
                     /translation="MALAAFGDPGHVFPVIALGSELVRRGHEVQVETWSKWRTDVEAE
                     GMRFAASPEYEVFPPDGRMLKPYQAAVRAAKETVPVLRAFDPDVVVVDILTAASSLAA
                     EMDGRPWATLVPHVLPTPGPGFPPYSIGARLPRTAAGRALWRSLDPFVQSGLRQGREQ
                     LNGARERVGLAPLNHLHNGISRRLAIVATFPHLEYPRPRAEPWVETTGPLLWEQPFGD
                     VELPPGDAPLVLIAPSTSQDPEQRMLAASLAGLADAPVRVLATYNRRPPPEPLDVPAN
                     ARLVEWVSYARTMPLCSAVVCHAGHGTVVRALSCGVPVVACPAAGDMAENAARVSWAG
                     VGVSLPRRLITPRGVRLAVERAIGEERIAARARELAAWSREHDGRVRAARAVEAFAAR
                     EAIGRA"
     gene            30843..31349
                     /locus_tag="VF517_04925"
     CDS             30843..31349
                     /locus_tag="VF517_04925"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX8052314.1"
                     /translation="MTDDPDTRDRRDDERRNDEENERLDRELMELVNELRVALTGVQI
                     LFAFLLTVPFTQRFATVTEFQRHLFFVTLLAAALAVVLLIAPTARHRLLFRQHDKERI
                     VSSSNRFAIAGLAALAAAMTGAVMLITDLLFKETMVSWVTAILAALFLVMWFVMPALR
                     RVAQGRGS"
     gene            complement(31507..32304)
                     /locus_tag="VF517_04930"
     CDS             complement(31507..32304)
                     /locus_tag="VF517_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052315.1"
                     /translation="MLADLLLRLLTSARRAENKVGFFLRRPSACSSASSTPGGTLRDL
                     NRKDLAMLKRMMVPAALGDALGTEPGFWDHARHVQLDPAYRRFADLYSRCSGRWQREE
                     PDTSDLALHIAGYYGNGPTRRRIIALYLREGARSLDEGASSTLAEIRLLQLGSDAYFP
                     ESPLSEDDQWRRPTAQYRDLIAAGSQAIAWRGAGKCIACGAGLPERTQPGTGAVVSRH
                     ARRNHCDNPEHCRRELRDVERWQTHAVDVVFDRCAYMLRSDPLEEAA"
     gene            32482..33186
                     /locus_tag="VF517_04935"
     CDS             32482..33186
                     /locus_tag="VF517_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052316.1"
                     /translation="MPAGNSKADNAFNRAEQWLSQRADEHLPTLDLADRSSDALGRVL
                     QAFNLPKRTPEQRDDEWRQTLAVINLGINAARSLRSMMVLLRHGYEPEAIALQRRLIE
                     AQARILRVIDPVSGPDHARQFLKGEDGTAGKAVSALPKDLWKGLNNAAHADYRAIEQH
                     LLSTDEEGSTRFYLVPRRRETASNMLLVLGAGHVLSAAAEIGKFKAIALDPLMDVLPD
                     FYSATERWLTPPETDT"
     gene            complement(33349..33421)
                     /locus_tag="VF517_04940"
     tRNA            complement(33349..33421)
                     /locus_tag="VF517_04940"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33386..33388),aa:Asn,seq:gtt)
     gene            complement(33412..33792)
                     /locus_tag="VF517_04945"
     CDS             complement(33412..33792)
                     /locus_tag="VF517_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052317.1"
                     /translation="MERSLRRVKRECRTHGEVDFVLEGRGYYRCTRCRMERVARRRRQ
                     IKELLVQEAGGRCVLCGYDRCVAALHFHHVDPSEKSFAIARRGHTRSLERAREEARKC
                     VLVCANCHAEIEGGAANLSALVAG"
     gene            complement(33854..34822)
                     /locus_tag="VF517_04950"
     CDS             complement(33854..34822)
                     /locus_tag="VF517_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8052318.1"
                     /translation="MNDLNSARLDDPEAPEREPRTAKVPDLFDEGGVVTAGVPAAQPE
                     GGADDAVRNYLKSIGKVRLLTKEDEVRLAKRVEANDMEAKNQLIEANLRLVVSIAKRY
                     TGRGLTLLDLIQEGNLGLIRAVEKFDWRRGFKFSTYATWWIRQGITRALADQSRTIRI
                     PVHMVERMNRVGRARRALNQKLGREPTPEEIAPEVEMTPTQVEEILELGREPVSLETP
                     VGPAEGESRLADFIEGDEAEAPLEVVSEGMRQRDLEAALKSLQWRERRVLELRYGLAS
                     DEPMTLEAIGTEVGVTRERVRQIESKTLSKLKNSREAEKLKEDIEQ"
     gene            complement(34819..36579)
                     /gene="dnaG"
                     /locus_tag="VF517_04955"
     CDS             complement(34819..36579)
                     /gene="dnaG"
                     /locus_tag="VF517_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX8052319.1"
                     /translation="MALYTKDSIERVKEAVDMVELVGARTDLKRVGSKWSGLCPFHDE
                     RTPSFTVDAEHKLYHCFGCGEGGDAIRFVESTEALDFKESVEYLADRYRVELKREQED
                     PQAEERRKRLERLQSLVERATAFYVRHLWETDEARTARDYLAGRGFGEAILREFRIGY
                     SPSAWDKLLTAAQRDGFSQEELAAAGLAQRGTRGGVYDRFRGRIMFPLADYRGRVLGF
                     GARSLREDQRPKYLNTSENELYHKGRQLFGIDRAASTAAKRGRIVAVEGYTDVLALHQ
                     AGITESVAIMGTALTQQQLTTLAQRVGKDGTVYLALDADRAGQDAMRRGARGARDLDL
                     ELRVVDMPEGADPADLIAGEGSGEFARRIDSARLVPDFEARRVVAQADLGSPRGRDRA
                     LEQVRPLVAALPANSATRDDLVGFLADRLDVPSDYVTSQVAAPQRPRPVAREAEPAAA
                     PLPTDAFERRERSFLAMCVAAGELGREYLERVDADYFSSDVLRRGRAHLVKHADDPLA
                     DLPIDDPPLTALITDVAMREQDGDPRSLALAFEQLALDRVQRQIRRAQRDGDLGRLRE
                     LGPEQMRIKDQINELMGQAV"
     gene            complement(36592..37323)
                     /locus_tag="VF517_04960"
     CDS             complement(36592..37323)
                     /locus_tag="VF517_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8052320.1"
                     /translation="MTDAAALAERRRRRLWLFGASALLVAILIGIGIGVSRTGGGTGT
                     TTGPPEGVAEVQALYRGIPQRGPLLGDSGAKVTLAEFADLQCPFCGEYSREVMPEVVR
                     RYVRPGKVRINFRGLDFIGPDSTEAMRFAAAAGMQGRLFQVVDLIYRNQGAENDGWVD
                     DEYLGRIGRAAGLDVPKALAARSGMEPTQQIDAAKAEGERAGVEGTPTLVILRAGSAA
                     TFRKLEADEVSLGEVTKALEAALGD"
     gene            complement(37320..37484)
                     /locus_tag="VF517_04965"
     CDS             complement(37320..37484)
                     /locus_tag="VF517_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052321.1"
                     /translation="MVYLAHGAGGHWVIDLLIYLGPFLSFIAVVLFTDRRRRKREQAA
                     AAEGASAENS"
     gene            complement(37522..38526)
                     /locus_tag="VF517_04970"
     CDS             complement(37522..38526)
                     /locus_tag="VF517_04970"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX8052322.1"
                     /translation="MPVVSTPEFHDRLRAWEDANLSPRAERSYPARRAVAEEDSPMRT
                     PFQRDRDRIVHSKAFRRLKHKTQVFISPEGDHYRTRLTHTLEATGIARTVARALALNE
                     DLTEAIALGHDLGHPPFGHVGESVVDACLRERYGFGFRHNHHSLRVVERIERDGAGLN
                     LTEQVRDGILRHTGPEKPATLEGQIVRLVDRIAYINHDIDDAIRAGVIVREELPVEEI
                     EVLGETGSRRIDTLVRDLVERSADAGAIVQGPEVGAAMDRLRAFMFDRVYLGATARSE
                     HPRIERMLRTLFAHYAENPPPALVAGASEAERVVDYLAGMTDRFAVRVFSDLSVPQGF
                     "
     gene            complement(38561..41206)
                     /gene="ppdK"
                     /locus_tag="VF517_04975"
     CDS             complement(38561..41206)
                     /gene="ppdK"
                     /locus_tag="VF517_04975"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX8052323.1"
                     /translation="MGAPSKLVYDFHEGSREMRDLLGGKGANVAEMTRVLGPDRVPAG
                     FTITTEACVAYLSAGKVEPADLEEQVSCALERLEERAGKRLGDPADPLLVSVRSGARE
                     SMPGMMDTVLNLGLTNSSVEGLAAATGNERFAWDSYRRFAQMFGNVCRGVPGELIEEA
                     IAERKRSAGVKLDTELSVDDLRALTDSFKELYRAQTGEEFPQDPREQLRHAIRAVFES
                     WTGARAVEYRRINRIPDDWGTAVNVQQMVFGNKGDTSCSGVGFSRDEITGAPEPSGDF
                     LVNAQGEDVVSGVRTPRDLSEMRDAMPAAHAELMDILRTLEGHYKDMQDTEFTVEEGR
                     LYMLQTRNAKRPAQAAVRFAVDAVGESLLTREEALATIDANSLDALLHPTFGRDADYT
                     VLARGVNASPGAAKGEIVFSAERAVEAAESGRAVVLMRPFTEADDVAGFWAARGIVTS
                     EGGKASHAALVARGMGRPCVAGVSKLEIDLASGEARVNGTVLHEGDLIAIDGTDGVIT
                     VDDVPLVEPEVDANFERVLEWSDEVRRLGVRTNADTPEDARRAREFGAEGIGLCRTEH
                     MFMAADRQPKMRAMIMAAEDEERDRWLADLLPLQQRDFEGIFEVMAGLPVTIRLLDPP
                     LHEFLPDVEELSHQVERARIEQTPDLEELERTLDRVHVLHETNPMLGTRGCRLGIVHP
                     EIYAMQVHAIVRAAKAVRERSGDAPRLELMIPLVAYEAELEIMRKLVERVVSEEGGEG
                     LDLTVGTMIELPRACFVADRIADVADFFSFGTNDLTQTALGFSRDDVEGGFLSRYLEE
                     RIVDRSPFETIDKPGVGWLVRLAAWTGREAKPNLKLGICGEHGGDPESIDFFHMAGLD
                     YVSCSPYRVPIARVAAAQASIARGG"
     gene            complement(41218..41937)
                     /gene="recO"
                     /locus_tag="VF517_04980"
     CDS             complement(41218..41937)
                     /gene="recO"
                     /locus_tag="VF517_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX8052324.1"
                     /translation="MPPTFTTEAIVLRSIRYGEADRVLHLYTRDRGRVGAIAKGVRRV
                     KSRFGGRLEPMVRSELVLREGRGDLCTVQSAETVDPYPALRESRAALDRAVEACAAVL
                     RLLDSQEPNQPAYNLLCHELHLLNTDPAAANRAQALAFRIKLLLAAGFTPELTSCASC
                     GEREHLSAFSASAGGVVCPGCEAGSFPLDGAAHAFLAEALGRPLADYPTGSEASLRQA
                     DRAIAETMEYHAHVRLRRVAA"
     gene            41956..42219
                     /locus_tag="VF517_04985"
     CDS             41956..42219
                     /locus_tag="VF517_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX8052325.1"
                     /translation="MADTTEIIVYTTEPCGFCRQAKALLQSRGVEYREINLAKDAEGR
                     ADLVAQTGQMTFPQVVVGERSIGGFRELLMADRSGQLQSLLAA"
     gene            complement(42233..43069)
                     /gene="mutM"
                     /locus_tag="VF517_04990"
     CDS             complement(42233..43069)
                     /gene="mutM"
                     /locus_tag="VF517_04990"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX8052326.1"
                     /translation="MPELPEVETIRRALAPVVEGNVLRSLEVLDPLWCDPAPPEEVAA
                     AVDGRRAERLTRRGKWLDLELADEVHLIMHLRMTGNLLVAEEAPYTRVRFDFGSERVL
                     FTDPRRFGTGVVLLGDDARDEYFSTRVGVEPLDPEFTADALRAQARGRKQPVKAFLLS
                     QERVAGVGNIYADEALFRAKIHPLRPVGTLRRPQIEELREAVVRVLNDGIDAKGASID
                     DFRNPDGAKGSFQDRFLVHLREGEPCVRCGRTIVKMRAAGRGTYVCPGCQRRPRVQKR
                     DT"
     gene            complement(43088..44032)
                     /gene="xerD"
                     /locus_tag="VF517_04995"
     CDS             complement(43088..44032)
                     /gene="xerD"
                     /locus_tag="VF517_04995"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX8052327.1"
                     /translation="MATITHIAEAPPYQHLVLDFLAYLEFERGLSRNTLEAYRSDLLQ
                     FGRFLEERGRGADDASSADVSDFLAELAAGDGNGRAPSSPATIHRKAACLRSFYRHLR
                     REGVRDSDPMATIAGPRRGRRLPKVLTRGEIGKLLEQPKGTEPAALRDRALLELMYAC
                     GLRASEAIGIEVRDIDLEDGVLRARGKGSKERVVPVGTKALDAVRRYLERGRPALVRG
                     RPVTHLFVNFRGEGMTRQGLYKIVRRHAAAAGLADRMSPHTLRHTFATHLLAGGCDLR
                     SVQEMLGHADVATTQLYTHLSSERLKDVYFKAHPRATA"
     gene            44136..>45829
                     /locus_tag="VF517_05000"
     CDS             44136..>45829
                     /locus_tag="VF517_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8052328.1"
                     /translation="MASNVSEREAREVAEAARETEWKLPSFGKGLFLGDFRLDLIHPQ
                     PPLGDEAVEKGERFLARLSAFLEEHVDPLQIERDARIPDEVVQGLKDLGAMGMKVPEQ
                     YGGLGLSQVYYNKALALVGSSHSAISTLLSAHQSIGVAEPLLLFGSDEQKQKWLPLVA
                     KDHVSAFLLTEPDVGSDPARLGTIAEPTEGGYLLNGLKLWATNGAIADIVVVMAQVPK
                     SEGHRGGITAFVLDYKSDGVTVEHRNAFMGLRGIENSVTRLENVFVPAENVIGREGMG
                     LKIALSTLNTGRLALPAICVGAGKWATKVAREWSTQRVQWGQPVGKHDAVAQKIAFIA
                     ASAFGLEAVVDVSGRLADDKKNDIRIEAAIAKLYGSEMGWKIVDELMQVRGGRGYETA
                     ESLLARGEKPIPVEQVMRDMRINRIFEGSTEIMHLLIAREAVDKHLEVAGEILEGDGG
                     LGEKAKVAVEAAKFYSRWLPQLAAGKGQNPRAFDEFGSLAKHLRFVERGSRKLARSTF
                     YAMTRYQAGLEKKQAVLGRIVDIGAELFAISAAVVYATTIGAQRPERREEAIELAQL"
BASE COUNT         6842 a        16265 c        15995 g         6727 t
ORIGIN      
        1 acggcgatgg tggcgctggc gctgccggcg ctggcgctgg cgcacatcga gcgtccctcc
       61 tactggccgg accccgcgcc cgacacgtcg gtcacgccgc cggccggcgg caaggtgccg
      121 aaggcgcgcc ccctctcgtc cgcgctggtg gaccggctgc ccggcgagac ccgcgtcgtg
      181 tgccagaaga agtcgctgac ctacctgaag aggtcgatcg cgaaggcccg caaggacggc
      241 tacacctacc gccccagcga gcacctgaag ctgagcgcga agcgcgccga gcggctcatg
      301 atcatcaacc gccgcctctt caagcgctgc aagtacaagg agatccagcc ggcggtcacg
      361 gcctcgacca acaacgaccg cgtggtcgtg atgcccggca tctacaccga gccgaccgcc
      421 cgcaaggcga agacgttcga cccggcctgc gacaagtggg agatcaagac cgagttcggg
      481 gatcccggcg cgctcgcgta cgactaccac ctgaactgcc cgaacgacca gcagctcatc
      541 gcggttctcg gccgcggacc gaacaccgcg ccgccgcccg aacctccgcg atacgaccgc
      601 cacgggatcc cgaacatcgg cccctgcatc cgctgcaact tccagatcga gggctccggc
      661 gtaagcgccg acgacacgat cgtcgacgcc ggacgcgtcg agtccggcaa cggcgcgccg
      721 atcgacgcca agaaggacgt ggggatccgc gccgaccgcg ccgacggctt cgtcctgcgc
      781 atgatgacct tccgccacgc gagggagcac ggcatctacg tgatggagtc ggacgggtac
      841 ctgctcgacc gattcaaggc cttctacaac gggctctacg ggacgctgac gttcgtcgag
      901 gaccacggaa tccagcagca ctgcgagacc gcgggccacg ccgactcggg catctacccc
      961 ggcgctcccg tcgacaccgg cgagcagcgg cccgagggca cggagttccg ttacaaccag
     1021 cagatccgct tctgcgactc gtaccacaac ctcgccggct actccgcgac gagcggcaac
     1081 gcggtccacg tccacgacaa caacttctac gggaacgcgc tcgggctgaa caccgacgtc
     1141 gcgaccgcgg caggccaccc cggctacccg ggcgactcga tgctctacga gcacaacaac
     1201 atctactcga acaacttcaa cccgtacgag gagggctcgg acgtcatccc ggcgttcccg
     1261 tacccggtcg gcaccggcct gtggatcgcg ggcggcaaca accacaccgt tcgcaacaac
     1321 aacatctacg acaactggcg ccgcggctcg atggtgttct cgatccccga cgtgctcatc
     1381 tgcggggagc agacggacaa ccacaaccag aagggctgcg acgagtcgcg caccagcacc
     1441 tcgtaccgga acaagcacta ctcgaacgtg atgggccgcg ggccggacgg gaagccggct
     1501 cccaacggca ccgacttctg gtgggacgag ttccccggca acaccgacaa ctgctggtac
     1561 ctgaacgagg gccctgcgcc gatcacgtac tcgcccgagc cgctgccgaa ctgcaacgac
     1621 ggcaagaact ccgagcagag cgtcggccac ggcaacctcc agaacgagtc ggagctcgcc
     1681 tcgtgcctgg cggcgttcga gacgcggaac ttcgatcagg agacaacctc ctgcccgtgg
     1741 ctgttcacgc cgcccaagcc gggatcctcc ggggcgctcg cgcaggacag cgacgccggg
     1801 caggcccgcc agctcgagct gttcctgcgc tactgcgcgg agaacccgca gagcaacacg
     1861 tgcaaggcgt acctctctcc gctcggcctc aagcgctgag cgcgcgactc gtcccggtgc
     1921 tgctcgcctg cgcgctggcg ctgggcgcgt gcggcgactc gtcggactcc gactccaagc
     1981 ccggcggccc ggccccccgt cccgccaacc tcccccccga cttcaacatc cagctcttca
     2041 actgcgcgaa ctggcagcag tcggacgaca agatccgcgc gtacgtggtc gaccgcctcc
     2101 agcagatcgt cggcggccag atcaccggcc agggcgccga cgggcgtggc tcgacgatca
     2161 ccgacgagca ggccacgcgg ctcttcgacg ggacgtgcgg ctcgccccag gcgcgcggct
     2221 tcctgctcta caagctctac gggcacgccg ccggcttcgg cgggggctag ccgctcacgc
     2281 ggcgagtgcc gccgcgcgtt cgcccgcccg cggcgcgagc cgctcgtcca tcaccacccg
     2341 cccgccgcgc gtcggcacca ccaccacgtg gtggagccgc tgcgcctcga gccgcggcga
     2401 cgccgcccgc aggcgcgcgc ggtccagcac ggtgcgcacc accaccttca tctccaggtg
     2461 cgcgaacgcg gccccgatgc agcgccgcac tccgccgccg aacgggatga acgtgtacgg
     2521 ctcgccctcg ccctcgagcc agcgctcggg gcggaactcg tgcggatcgc tccaggtgtt
     2581 cgggcgcagg tgcagcagcg gcagcgcgat cacgacctgc gtgccggcgg gcagcgtgta
     2641 gccggcgacc tcggcctcgc tcttcaggat ccgcgtggcg tcggtcgccg gcggacgcac
     2701 gcgcagcgac tccttcacga ccgcgtcgag gtagtcgtcg tcctcgggct cggcgagcag
     2761 gcggtcgagc acgcgcggtg tgcgcaggat ccgttcgaag caccaggtga gccccgccga
     2821 ggaggtctcg tacccggcgg ccagcagggc gtacatctcg tcgcgcagct cctggtcggt
     2881 catcgcgccg ccgtcctcgt agcgcgactc gatcaggagc gagaccacgt cgccggccgg
     2941 gtcgggggcg gcgcggcgct cggcgatctc gcggtagacg agctcgtcca tcgcatcgcg
     3001 cgcggccacg aagcgccgcc aggggctcca gcggccgagg tcgcgctgga gcggcggcag
     3061 cggcaggacc actccggaga ggcggtcgag ccggaggatc cccgcggcga gctcctcgag
     3121 ccgcgccggg tcgcgcactc ccatcaccgc gcgcaggatc gcctccatcg tgacccgctt
     3181 catcggcggc agcagctcga acgcctcgcc gaccggccag cgttcgatct cgcgctcggt
     3241 cacctcggcg aacacgtcac tccagcgccg cagcgcctcg ccgtggaagg gcggcagcag
     3301 cagcttgcgg cggcgcaggt gctcgccctc gtcgagcgtc atcgacgaca ggcgtccgat
     3361 cgcgggctcg aacagcgcgg cgttcccctc acccgcgtgg aacacgtcgg cgggcccctt
     3421 gaacacacgc tcgacctcgc cggggtcggc gacgtagacc acctcgccga tcccctggaa
     3481 gcgcgcccgg aagcagtcgc cgtagcgggc ctggcagcgc agcaggaagt cgatcggacg
     3541 gcgcatgaaa gcggccgtct gaagcacccc gggaacgcgc gggccgggcg gtagcgccat
     3601 gggcggaggt tctactagag aatgccgcgc gtgacagccg ctgccacacg cgccggactc
     3661 gccctcgggg cgctcgccgg cgcgctgctc gtcaatcgcg agctggccgg gcgggaggcg
     3721 aagccggccg aggcgcgggc cgggctgctc gtgccgaccg cgctgggcga gctgcacgtg
     3781 ctcgaggagg gtgaccccag cgcgccggtg gccgtgctgc tgcacgggtt cggcgggtcg
     3841 ctgcactggt tcgaccggct cgcgccgctg ctcgccacgg accatcgcgt catccgcatc
     3901 gacgccctcg ggcacggcgg gtcggccaag ccgcggatcg actactcgtt cgaacagcac
     3961 gcggccgcgg tggccgaggc gctcgggcga ctcggcgtcg agcatgcgct gttcgtgggg
     4021 cactcgttcg ggggcgcgct ggcgatcgcg gtggccgagc gccggcgcga gctggtcgag
     4081 cggatcgtgc tgatggacga cggccccgac aacgacttcg gcgaccagcc gctcatgacg
     4141 acgatgggat tcgtccctgt gatcggggag ctgctgcggc ggctcgcgtt cgactcggcc
     4201 atccgcgacg gcttccgcga cgcgttcgcc gacggcttcg acctcgccgg cggcttcgac
     4261 gaccccgacc aggtcgtgca cgactaccgc gcgatgacct tcacctcgta cagggggtcg
     4321 tgggacgccg aggaggctta catggacgcg accccgctcg acgagcgcct gcgccggctc
     4381 ggcatgccct cgctcgtgat cttcggcgag cacgaccggt tcttccgagc cgaggaggcc
     4441 gccgccgcgt accggcgagt gccggggctg cgcgtggagg tgatcgccgg gtccggtcac
     4501 tcgccgaacg tcgagcggcc ggaggaggtc gcgcggctcg tgcgcgagct cgtcgcgggc
     4561 gccgcggcgg gatgatcggg cggcgaccgg acctcgggcg gcggttcggc ggcacgcacg
     4621 cggtcgtgac gggcgggtcg gccgggatcg gcctggcgat cgcgcgccgc ctggtcgcgc
     4681 tcggcgggtc ggtcacgctc gtcgcgcgcg acgaggagcg gctcgggcgc gcggccgcga
     4741 gcctcggcga gcgcgcgcgc acgctggcgc tcgacatctc cgacgccgac gccgtcgcgg
     4801 accggctccc ggcggagctg gcggagtggc cggccgacct cgtcgtgaac tgcgccggca
     4861 tctcgacggt cggcgagttc atggacctcg atggcccggc cctgcggcgc gagatggacg
     4921 tcaactactt cggcgccgtg tgggtcacgc gcgcggtcct cccccacttc ctcgagcgcg
     4981 gccgcggcca cgtggcgatc gtcgggtcga cggcgtcggt gatcggcatc cacggctacg
     5041 gcgcgtacac gccgccgaag ttcgcgctgt acgggctcgc ggaggtgctg cgggcggagc
     5101 tcgcgccgaa ggggatcggc gtgacgatcg tgctgccgac gagcactcgc accggcatgc
     5161 tcgagcgcga gctggaggag gcgccgccgg agacgaggcg cctgatcacg gcggagcgca
     5221 tcctcgagcc cgaggaggtg gccgaggccg cgctgcgggc ggtggcgagc ggccgtttcg
     5281 aggcgatccc cggccgcgac gtgacgctcc agacacgcgc ctaccgcctg gcgccgagcg
     5341 tgggccgcag gatcgtggac cgcaaggcgc gcgggtgagc gacggcggcg cgccgggcgt
     5401 ggccgctgag tcggcgccga gcgcttcacc gcgcgcggct ccgggcgagg ccggcgcgcg
     5461 cgagctgatc gagaccctcg cccggctcga gcggcggccg tgctcgccgg gcgagcgcga
     5521 ggccgccgag tggatcgccg cgcgcctcga gcgcgccggc tgcgagcgcg tggacctcga
     5581 ggaggaggtc gcctacgggc tctacgcccc cacgctgacc gcgctcggcc tgctcggcgc
     5641 cctcgcgtcg ctcctctgca tcgccgggcg gcgcgtcgcg gcgagcgcga tctcgatcgc
     5701 cggcctcgtc acggtcgtgg acgaggccca gaacgggccg cggctcgtgc gccgcgcgct
     5761 gtaccccgcg cgcaccacca ccaacgtggt cgcgcggatc ggccccgacg acgcgccgcg
     5821 cacggtcgtg gtcctcgccc accacgacgc ccaccagacc ggccgcctct acgaccagtc
     5881 gctcctgatg gccgcccacc gccacgcccc cggcctgctc gcccgcatga agacctcgct
     5941 gccgcagtgg tgggtcggct gctcgggcgc ggtgctcacg ctcgtcgccg tggccacccg
     6001 gcgccggcgc cacgccgccg ccggtgcggt gctcaacctc gtcggcgcgg cggcgatggg
     6061 cgaggtcgcg cgcaacccgg tgagcccggg cgccaacgac aacctgtcgg gcgtcgccgg
     6121 cctcgtcacg ctcgccgagc tgctccggga gcgaccgctg ccggggctcc gcgtgctgct
     6181 cgtctcgtgc ggcgccgagg aggcgctcca ggagggcatc cgcgccttca tggctcgcca
     6241 cggcaacgag ctcgacccgg cgcggaccgc gttcgtgaac ctcgagacgg tcggctcgcc
     6301 gcggctcatc atgctcgagg gcgaggggcc gatctggatg gaggactacg ccgaaccctc
     6361 gtttcgcgac ctcgtcgccc gctgcgccga ggccgagggc atcgagctcg aacgcggctt
     6421 ccgcgcccgc gcctccaccg acagcgtgat cccgagtcgc gccggccatc ccacggccac
     6481 gatcacctcg atcaccgcct ggggtgcgct cgccaactac cacctcccga ccgacaccgc
     6541 cgcgaacgtc gaccacgaca gcgtccagcg cgccgcccgg ctcgcgtacg cgaccgctgc
     6601 ggcctttcgc gcctagttcc cccagccggt ggggacgttt ccccccagag cggggatccc
     6661 tggtacggtc gcgaacggcc atggcgaccg agacacggga cgcgccggca gcgactcgga
     6721 gggctcggct cggtccgctg ctaggcgacc tacaggcact ccgccaagac ccgctcgggc
     6781 tcttcgcgag gggcgcggcg agcggtgcgg acgtggtccg cttccgcgcc gcccaccgca
     6841 acgtgtacct gctcgtgggg ccgggcgcga tcgcgcacgt gggcatccac aaccgcgaca
     6901 actacttcaa gggcgtgagc tacgacgccc tgcgcgtgcc gctgccgaac gcgctcctga
     6961 cgatcgacgg cgacgaggca cgcgagcgtc gcaagctgct catgcccctg ttcacgcgtc
     7021 gcagcctcct ccccgacgtg tcgacgatca cggccgccgt ggacgacctc tgccggcgct
     7081 gggacgagtt cgcccgcacc gggacggagt tcgacgtgca cagcgagatg aaccggctga
     7141 ccttcgacgt ggtgggccgc atcctcgtcg gcgccgagct gggcgacgag atgggcaggc
     7201 tcgaggcgag catcgcgcgg gcgagcgagt gggtcgcgcg ccgcacgcgt gcgctcgtgc
     7261 ccctcccgac ggcgatccca acgcccggca acatcggcta ccgccgcaac taccgcgcgc
     7321 tggccgcgtg ggcggacggg ctggtcgcct cgtcccgcgg ggcgaccggc gacggcgtgg
     7381 ccgggcggct ggtcgcggcg agccgcgacg gggccggcat ggacgaccgc cagctgcgcg
     7441 acgaggtggc cgcgttcctg atggccggcc accagaccac ggcctcggcg ctgtcgtgga
     7501 cctggtacct cctcgcccgc cacagcgggg agcaggagcg cctggcggag gcatgcgagt
     7561 tcgcgctcgc cggcggggcg cccgacatgg agcggctcga cgtcgtcccc tacctgcgcc
     7621 aggtgctcga cgaggcaatg cgcctctatc cgcccggctg ggccttcacc cgcacgccca
     7681 aggccgacga cgagatcgcc ggcgagcgcg tccccgccgg ctcgatcgtg atcgtctgct
     7741 cctacgccaa ccagcgcagc ccgcgcttct gggacgaccc ggagacgttc gacccggggc
     7801 gcttcgcgac cggcaggcgg gagtcgatcg gcccctactc atacttcccg ttcggggtcg
     7861 ggccgcacgc gtgcatcggc aagcacctgg ccgagatcga gtcgcggctc gcgctggccc
     7921 tgctcgcccg ccgctaccgg gtcgaggccg tctcaggcgc gccggtcgcg ccggagcccg
     7981 ccatcacgct caccccccgc acgccgatcc gcgtgcgcgt gacgcggcgc tagatcccgc
     8041 cgaggtagcg catccagatc tgcggctcgg cgcccgtccg gttggccgcc gagtcgaaca
     8101 cgacgtagtt gcggtagtag ctcgtgctga cgtgctcggc cccgctgctc agcgggtcgt
     8161 tgccgctgtc gaccgactcg aggatcgtca cgggctgggg cacgttcggc gagccgctcc
     8221 agaagtacgc gtcgcgcagg ccgttgcgat cgccgagcgc gccgctcgac ttgatcggga
     8281 ggttcgaggc gtcgctcacg aaggccgcgt tgaagccggc attgaagatg ctcggctcgc
     8341 gcgagtcgcc gttgccgacg tcgccgtccc aggagctgac gatccgctcg ccgccgccga
     8401 tcgagcgcag cccgatctgg ctgaaaccgc ccctgtcggt ctcgtagatc acggtccgga
     8461 ggaccttccc ctggcgcctg cgctggatga agccggggtt gcgcccgccc ttggccacga
     8521 gcgtgggctt gctcgcgccc tcggccagga ggtagacgcc gccggaggct ccgaacacca
     8581 gcccggtcgt gtcgccggtg tcgtacgacg ggtccgcctc ggcgcccttg acgtcgatcc
     8641 gcttggtgga cttgccgctg cgcacgtaga gcccgccgcc gctcacgaac gcgaccttcg
     8701 agcagtcgcc ggagaccgcc acgtgcgtcg tgtccgagct cgactgcttg ttcttcggca
     8761 gcgagacgcg cgtggggacg aacttcgggg ccttcgcgag gaacgcgtcg gtctgtccgt
     8821 tggtgtcgcc ggagacgagg ttcgaggcgt cggacaggaa cgcgacgcag cgggcgcggt
     8881 ccttggcgct gccgtcggtg gtcgcgtcga acgacgggcc gttggcgggc ttgccggtgg
     8941 cgctgcgcga gacgagctgc gtcttgccgg gggtccaagc actgccggtg tcaccgaacg
     9001 atccggcgcg gcgcacggcg aacacgtcct tcagcccgtt ggtgtccctg gcgacgaggt
     9061 cggacgcctc ggactcgtag gcgacgatct gcgagtagcg caggtcggcc gagatgtgcg
     9121 ggttcgtgga cggcccgttg ggcgtgttgc cgtcaggcgt gcgcgagaag agcgtggtgg
     9181 tggcgtcgcg gccctggccc accgaggcgt cgggcagggc catcgcgacg gcgatcagcg
     9241 cggcgagcat cagcgcgccc agggcgccgg gcagggcgcg cggagtgagt cgggccatcg
     9301 aatccactat tagctgtaac gcagcgaaac ggcaaatgtt cgggtctttc gctcggctag
     9361 cgtttcaggc gcagtcgcag tcgaaaggcg ccgatgagtg acgaagagag ctcgttcgag
     9421 tacaaacgtg tcggccagca cctgatccgc cgcccccggg cggtggagcg ctggcaggtg
     9481 ctggtggacg tccgcggctc ggctcggtgg atcacgtgca gcgcacgcct gagccggcgc
     9541 ttcgagcgcg ccgcgtcctc cgaggagttg aatgccgacc gcgccgccgg ccggatggtg
     9601 cgagcgtgcg tggccaaccg gcccgtcact gacccggcgt ccccgaatcc atccgtgcgt
     9661 acgcgcctgc gcgttcgggc cctgacctgg cgcctgagcc cgcccctcgc cttcggcggc
     9721 ccacggcgcg tcggcaccgc ggcggcgcgg gcagcacagg aactggccga gcgagacctg
     9781 cggcgtcatc gcggcagcgc cgaacgcatc gagggcgagg cccaggcggg ctgggcgcag
     9841 aaccgtgagc ggctcgcagg cgtcgagcag cgtgccgctt acttcctggg cggtgccggc
     9901 ctcggtgccg gagtgctgct cgcgtcgacc agccttctcg tggattccga cgctcggccg
     9961 gagggagtgg cccggatcgt cgcggcgatc gggctcctca tcacgatcct cggactggtg
    10021 gcgtcggcag cgtacgcgct cgccgcgacg atgcagacct tcgggcgcat cactcccgac
    10081 atcacctcac aggtcatcga gcgcgccaag aggcccgacg agcaggcccg catggagcgc
    10141 gccgcccggc tgatcgcctc acgccggcga tcgagttaca tcgcgaattg gaaggtcgcc
    10201 cgcctcacgc gtgcagcaat cgcatcggca tgcgccgtgc tgggattgct catcatgctc
    10261 accgccgtcc tggtgacgat ctgagcgctg agcggcgccg atccagcgcg gtcagcggac
    10321 gaggcggacg gtcctcctga ggggcgggcc ctcgttgccg gcgaggtcgc gcgccgcggt
    10381 cgtcacgccg agcgtcgcgc tcccgcccgc ggcgaggtga ccggccagcc gccgcgcctg
    10441 tgccttggtg agcctgaagc gaaccgttgc cttgctgccg gccttcgcca ggtacccggc
    10501 cttcgcgagc ttgactttcg acgacttccc cctgcgtggc ttgagcgtga gggtgccctt
    10561 cgcctggccc gagcaggctt cgtcgcacac gatcgtgagg tcgatccttc gggtggtgtg
    10621 cgcgcgctgc ttggtcttgc cgccgagtgc ggcgatcggg ggctccaggt cgggcggcgg
    10681 gggcggatcg cacgagcgcg gcggcgggtt gagggccgcc ggtgcgccct cggcgaagtc
    10741 ggagacctcg agctggcggc actgggcgtc gtgcatgcgg atccaggcca cggcgccagc
    10801 gccgctcgac gcggtcgcca tggcgggctg gtaggagtcg gagggcgaga gcgttagcgg
    10861 cgccgtgctc cacgccccgc tcgctccgtg caccgcgagg atcgcgccgg tgttctgctg
    10921 gcggtagtcg tgaccatgag gaccgttgcg ccaggtggcc agcgccgtgc cgtccggcgc
    10981 gaccgcgagc cgcccgtact ccgcgttctc gccctcggag ctcacggcga ccgggtactc
    11041 gaacgcctgg ccggggcggc ggaacgcgct gaacaggcgc cacgggctcc ccgcgtcacc
    11101 gccgttgttg atctgcgacc agagcagggt ggcgttgccg gcgccgtcga aggcgagctg
    11161 gggcgggctg aagaactcgg tgccgtaggc gtcgccgggg aggtcggcga cctggtccgc
    11221 cgcgccgaag gccgctccgg cgccggccgg gcgcacgcgg gcgttgattc ggcgctgctc
    11281 gtcgatccag atcgcgagcg tgttgccgct cgcgtccacg gccacgttcg gctggctcag
    11341 ggggccgacc ggggctccga ggtccgtcgg tccctcccag ctcgaggagc ccggcgggcg
    11401 caccgcgatc tgcgcggggc tgccctgctg gtgccagacg agggccatcg tgccgtcctc
    11461 cccgacggcg ccgcgcgggg agtccccgcc gctgccgatc gtctcctctg gctcgaactg
    11521 accgcccgcc gggcgccgga ccgcgcggac gcccgagccg aagccgcgtc cccacacgag
    11581 caggatcgcg cccgagcggg cgaagtcgac gtccatctgc aggccggggt tgatgcccgt
    11641 ggcgatctgc tgcggcgagc cgaacgttgt ggtgcccgcg gcgcgggtga cggcgtatgc
    11701 ggtctcgttt gcggaggtgg cccagatcac ggtgaggttg cccgcgccgt cgaagccggc
    11761 gaacgcgtgc gagtcgccca ggccgacgtc gccgacggtc tggggcgcgc cccacccgcc
    11821 gcccggcggg cgcgatgcca ccttcacctc ctggccgtcg aggtaggcga tggccgcgtt
    11881 gccggctggg tcgaaggcga ccgacgggag cgcggacgcg gtcgggccgg ccactgcggg
    11941 cgggcggaag ctcggcgccg cggtcgcggc ggcggcgcag aggagcgcga tcgcggtggt
    12001 ggcgatcgcg gcgaggatcc cgtgccgagg cgtcatcgag tggtcgaggg taggtccgga
    12061 tggcgcgctt gcccaacagt cgttaccaag acttaaccct cccctggcgg ttgggcagga
    12121 aacgggtggc aatctcgccg ccatgaagac gctaatcccc ctcacagcac tcgtggcgct
    12181 gctcgtcggc gccgctccca gccccggcaa ggacacggcc actcgagtca gccgctccgg
    12241 cgactgctcc ggcgcgtcga actggaagct caaggccaag cccgacgacg gccgcctcga
    12301 ggtcgagttc gaggtcgacc agaaccgcaa cggcgtcgag tggaaggtca agctgcggcg
    12361 caacgacaag acgctcgtca gcacgacccg ccgcacgcag gctcccagcg gctcgttctc
    12421 gctggagcgg cgcatcggcg atcccgccgg caaggacagg atctcggcgg tggcgacccg
    12481 cgacggcgag acctgccgcg gctcgctgag catctgagcg ggcgccgcta gaccggagcg
    12541 tgccagagcc cgcagagcac ggacgggacc gcgccggcgt cggccagacg gagcagctgg
    12601 tacccgacgc cggccgtgcc gagcatcagc gccggactcg gaccgccgca cggcgtgccg
    12661 gagcgcatct cggcctccgg caggcgtgcg atcgcgccgg ccgcgcgccg cgccgcgctc
    12721 gtgaagtccg ggcgacccag gacgtgcccg ccgatccaga gcgcctccac gaggcccgca
    12781 acgccgtggc atggcgtcag gtcccacacg tcgagggacg cgcggcgctc gagctcggcg
    12841 cgggtggtgt cgagccccgc cgtgacgtgc tcgtcgtacc aagcgcgccg cgccgggtcg
    12901 agctcgcggg cgcgcaggcg gctcagggcg acgccgggcg cgccgtggca ccaggccacc
    12961 gcgtagcgcc gctcgcccgg cgggtggagc tcgtcggtgc gcaggtcggg ccagttgccg
    13021 gcgtccgcgt cgaagaggcc ctgctcgtac tcgaacgcgg cgtgcgcggc atcgagggcc
    13081 tccgggtagt ccccggcgac ggccagttcg agcagcgcga cgcccacgcc ggccgctccg
    13141 tgcgagatgc cggtgagcgg cggctccgcg cccgccgcag gggagtcgtg cccggcgtac
    13201 gtgaccgcgc cgcggccgag ccggcggccg atccgctcgg cgtgcgccac gtactcggcc
    13261 cggcccgtct cccgcgcgag cgcgagcagg gcgaggacct cccccgcccg gcccaccagc
    13321 aggtcgtcgc gggcgtcggc gcagtgatcg gagagcgagc cggccagcag gtcggcgtcg
    13381 ccgccgtccg gaggctcgtc gccgagaagc cgcgccactc gccacgcggc aagcagcgtg
    13441 cccggcacgc ctgagtagag gccaaggctc gtcgagctgg ggcgttctcc gcgcgcccgc
    13501 gcgagggcga atgcgaccgc tccgcgcgcg gtgcgcgcga actccggctc gcccgtgcgc
    13561 gcgaacagct ccgccaggaa cagggccacc ccgctcgtgc cgtcgtagag gcccggcccg
    13621 aggccggtcg agcgcggcac gaagagggcg accgagcccg ggcggccgaa gctcgagcgg
    13681 ccgatccagc cgcacgcctc gccgcggcgg tcccacaggg cgtcggcgac gatcgtctgc
    13741 ccgagccgcc ccgccgccgc gagcagctcc gccgggtccg cgtcatcgga cggcggcgag
    13801 tcgcggtgct ccgcgaccgg agcggccttc cccggcagcg gctcgtaccc gtcctccgag
    13861 ccgggcgcga ggtgggggcg ggccggatcc aggcccgcct cggcgaaggc ggcggcgatc
    13921 gccgctcgct tcgacgccgc atcccggtga ccgtcctcgt gtgctcgcca cagcgcacgc
    13981 gcgacgaggc ggcagcggtg ctggccgaag ctctcgccca ggccgggatc ctccgctagc
    14041 gcgagccccg gcgcgagtcg cttggtgaag agcgggacgc tcgggcgcag gcggccggca
    14101 agctcggcgt acaccccccg cagcggtccg cgcgcctcct cgaagcggtg gcgcggcacg
    14161 tacagcacgg cggcgtccgc gcggcgatat gccgccggac tgcgcagcac cttgagcttg
    14221 aaggggacgc gcgcgccgtc gagcgcgctc tggaccgtcg caagaagcgc ggcggcggcc
    14281 tgctcgcgca cgtgccagta cagacgaacc attcccgcgg gctcgtcggg ggcgtcccac
    14341 gcctcgcgtc cgatgaccgc gaagtagccg ggcgcgagcg cccgccgcac ggtgggcacc
    14401 aggaccgcgc agccgtccgg atcaccgtcc gcgacgccgc gcacccgctc gggcgccacc
    14461 cagaagcgga ctccgtgcgg ggcgaccacg gtcacgcgat cgccctcggc gcccgcgcga
    14521 cgccagccgg gctcccaggt caccccgggc cgggccgcgc ggatcagcga ctcctggaag
    14581 tcgcggtccg tgacggcgtc gaggacggtg atcggcgcgc ggccgtgcgg gcggacgtag
    14641 tggtcgcgat acagcaggct cgcaagggtg gggacggggt cggtccccgg cgacgcgctg
    14701 acagcggagg cgaccgccgc gagatcctcg tgcagaggcg cgtgggcggc agtcaccaca
    14761 gggcgagcag ctccgcggcc gcgcgctcgg gctcggcgaa gacgttgtcg gccacctgga
    14821 gcgcgatgag ggccgagccc gagagctcgt cggcgccggc caggagctcg agcgtggtct
    14881 gcacgagccg ggccgcgcag tagtcggtcg cccgcgcccg gagctcgccc tcgccggagg
    14941 agccctcgcc accagcgccg atgtacgcgc gccacaccgc gcgggtcgtc gcctggatca
    15001 ccggaaccgc gagctccgcc gcggggtcat cgccgcccca ggtctcgccg cgcccgagcg
    15061 cgagcgtgac ggccgcctcg agcaggccgg ccagatccca ggcgggatcg ccgagacgtg
    15121 cgagctccca gtccacgagg cgtacgtcga tcccgccgtc cgcgtgcgag cgcaccatca
    15181 cgttgtcggc ccgcacgtcc ccgtggatca gcgactcgct gcgccacacg tcggcggcgc
    15241 gcgccagccc ggcgtcgatc gccatcgccc gctgcaccac ctcgagaccg gtcatcgcgc
    15301 cgttgctgag cgtctcgagc gcggcgggat cggggcgccg cacggcgggc acccagccga
    15361 gcggcgtggc ctccggcccg tcgggccggc cgtcggcgcc gacggtcacc gtgtggacct
    15421 gcgcgagcgc cgcggcggcc gcgcggaacg cggcgatcgg gaactcggcg ggcccgccgg
    15481 cctggcagac cgagcgcagg tccccgtagc ccacgagcag ctcggtgacg aggagaggct
    15541 ccgacgggtc gaagtggacg agccccggga gcaccgcgga aagcgggcct ccgccgcgat
    15601 gagcgtggta gacggctccc tcggcgatca ccgtctcccg gctgccctgg gcgcgcgggt
    15661 cagcctgctt cacgaacacg ccggcgccgc cggagagcgc gacgcgcgtg ttgcggttgc
    15721 ggcgcgggca gcgctccacg gccgtgtcgg gcaggatggc gtcgtcggac gcgagcaccc
    15781 ccgcgcgcac gagccgccgg accagggcgg cctcgctcac gtgccggcgc gaagcggtcc
    15841 gcggcggcag cgcagccggc gtcgcagcac caggtgaaga tgacgccgct ggtcggcggc
    15901 gggcacggga cacccgcgct catgcaccag tcgctcccgc agccgctcga ctgcggaagg
    15961 cacatccccg agcaggccga cgggtcgccc gtgtcgggga ggacggccag ctcgcgggag
    16021 aagttcgccc ggaagctctc gcgaagggcc ccggtgacga aggacgcgct gccagagatg
    16081 tcgcgggtcc gcaccagcct gagctgagct gacggcttca cccacaggat gcgcgggctg
    16141 tccgggtcgc cgtcgtcgac ggacttcgtc cgcaccacgt cggccgtggc gacctcgtag
    16201 tagctcgcga acgacaggtc gaggtacagg cgcacctgcc ccccgttcgg ggagctcccg
    16261 acgaatccgg cgatctggag gagggactcc cccgcgttcg catcgtcggc gccgtcaggc
    16321 gccatcgagt gcggcgtcag gtccacgtcg tgcgcggacc ggtcctcctc gctcatcgca
    16381 tcctccccct gccggtagcg gacgaggcgc gaccgtaaca caggggtccc ccgcgcgggg
    16441 gattgcgggg gcggagtggg ctgggctgga gttcggacca ggatttcgcg gccgagatgg
    16501 atcaggtggc taaccgggcg gccttgttgg atgatcgcta gatcgagccg ccggcagcga
    16561 atggcctaac agcggagccg acgcgggttc ggttcaatcg ggaaggcagt tccatcgtgc
    16621 tgggctcagc tggttagacg cgaaacgcgg gacgttcacg cagcggtcga ggggctcctg
    16681 gaagagcagg tatcccgctc atcggtggag aactacctgg ctaggaagtc ggtcggccat
    16741 gctctgcgat cgggtgggcc cggggtcgct accgcttggc cctagcccgc gacactgaca
    16801 ccgctgcttt cacgcgaaag cagcagtcgg ctgccgcacc cgaggcgtac ccccgccgcg
    16861 ggtcggaggc catgcgcggc ggcgatctcg tggcgaaggt aacctcaccg tgaccggtcg
    16921 cagtcgtcca accctgcgcc agggacgatc gaaccgctca gccgagcgtt tgggtgctgt
    16981 tcgcttgaaa ccagctgcga ccccgcgggt ggtcacttga acgttcacgt ggcaggagac
    17041 tcgacccagg cccctgccgc cagtccctcc aggcttcgcg tccggccgag atcgcattct
    17101 cgcggtgggg ctccgcagca agtttctcta catgtctcat tgcgtcgttg gcgggcccag
    17161 tcggtctcgc ggacgcgcag gagtccaccg cgcccactcg tcccgcccgg agcggcgaac
    17221 aatcctcctc aacgtacgca tggtcagtat tccgctttcg gggtgcccca gcccacagtg
    17281 cgcgaggcta cctacagggc ttcagcacgg cgaggacgaa ggagagggcc gcggcgcgcg
    17341 ccccgcaagg cgtcttgact gggcagggat tgctgactac gttggcgcgt tctcgaattc
    17401 tttcatgcac ctcaagacct tccgcatcag aaacttccgt cgactgcgcg acgttcgcat
    17461 cgacctcgac acggctacga cgatcttcgt cggcgccaac aacagcggca agaccgctgc
    17521 gacacacgtc ttcaggacct tcctcaggaa gagccccttt cggatctacg acttctcggc
    17581 gagcacatgg caactgttcg acgagttcga cgtcgaggcc ggtgatcccg acgcagagct
    17641 tcctcgcatc acgctcgatc tctggttcga ggtcgaggat gcgaacctgc agcgcgtcat
    17701 caaccttctc cccagtctcg actggaacaa cgagccagtc ggcctacgca tcgagtacgc
    17761 gccggtcgat ggccgtgagc tggtcggccg ctaccaacaa gtcctgaacg agcgccctcc
    17821 ggacccggat gtgccgactg cgagctggca accctggccg cagagcctca cggattacct
    17881 ttccaagcgc ctcaccgacg agtacgagct tcgctaccta gtcctggacg ccaagagctg
    17941 tgacgaagag tttcgcttcc catcggacta cgagccgcta cggctgccaa ccgggcagag
    18001 cggcgcgagg ctcgtcgatt ccctcatccg ggtcgacttc cttgatgcgc aacgccacct
    18061 aaccgacaca gaagcgcatg gacgcgcgga agagctgtcg aaacgccttg cccgcttcta
    18121 cgaacggaac ctcgagcagc acgaccagga tgtcgcggcg cttagcgccg tggctgagac
    18181 cgaagcccgc ctgaacgcgc acttcgcaga ggtctttgaa cccacacttg agcgcttgca
    18241 acggcttggg tatcccggct tcgcggatcc cgaactcgtc gtccgcacag cgcttgacgg
    18301 tcagaccgtc ctgaacagct cggcccaggt cttctacaag ctctcggagg gactgaaggg
    18361 cggcgaaggc gcgcgcgtgc tgctgcccga ccagtacaac ggcctcggtt tcaagaacct
    18421 catctacatg gttgtggagg tcctcgactc ccacttcgcg tggcatgcag cggacgacgt
    18481 gcttcccccg gtccacctcg tgatgattga ggagcccgag tcacacctgc acgcgcagct
    18541 ccagcaggtg ttcatccgca agatccgtga ggtcctgccg gaatccgagg aaggactcga
    18601 aacgcagatg gtcgtcacga cgcactcggc gcacgtcctc tacgaaagct cgttccagca
    18661 catccgctac ttccgccgca gcgtcccgcc ggccgacacc cgagagagcg acgtgcgcaa
    18721 cctgtcgctg ttctatgacc gcgaggaagc cccgacgcgt aggttcctgt tgcagtacat
    18781 gaagctcacg cacggcgacc tgttctttgc tgacggtgcc gtgctggtcg aaggcaatgt
    18841 cgagcggctc ctcgtgccgc tcatcatcga gaaggatgtt cgcgagctgc gatcgcgccg
    18901 cctcgccatc atcgaagtcg gtggcgccca cgcgcaccgc ttcaagcagc tcatcgagtt
    18961 cctcgactta ccaactctgc tcatcgctga cctcgacagc gtcattccga acgagcagac
    19021 cgggcaatcg cgctcctgca tgaccacgga ggcggacgca acgaccgcga acccgattct
    19081 caaagattgg cttccgcagg tcgagctgat cgccgagctg ctcacgctcg acgccaccag
    19141 aaagatttca atggcggagt ccaacggcgg cgggcaggtt cgtgtggctt accagacgac
    19201 tgagaccgcg gattggtcgg gggagtcgaa gatgctcgcc ggacgcacgt tcgaagaggc
    19261 cttcgccctg cagaacctca attggatcca gagctcgaac caggtcgcgc tcggactcgg
    19321 cgtgaaggac gccgcgcaga agacactcga tgacctccac gaggccatct acaacaaggt
    19381 ccgcacgttc gacaagacgg cgttcgcgct ggctttgatc gacgcagacg agaacgcgtg
    19441 ggtggcgccc gcctacatcg tcgagggcct cgaatggatg cgcggtgtgc tggtcgaccc
    19501 cacctcgggc agcgaagacg atggtcagcc cgacgccgcg gtgacggctt gacgggacgt
    19561 gccggcgctc ccgataccga cgcggacgtc gagatcaggg cgctgctcga cgaaacgccg
    19621 cgccgcgcct tcgtagtcat agccggcgcc ggctccggca agacgacgtc gctcgtcaaa
    19681 gcgctcgccc acgtcgcccg gcgggacggt ccggcgcttc gcaaccgcgg gcagaaggtg
    19741 gcttgcatca cctacaccga gatcgccgcg agcgagatag cggaggacgt gggggcggga
    19801 ccgctcgtgc acgtgtcgac aatccacagc ttcctgtgga ctctcgtccg gccgttccag
    19861 aaggaagtga tcgcgtgggt cgcggacaag atcgatcgga agatcgctga agctgaggcg
    19921 aagttgcagt ccgagcgcgc aagagagtcc acgaaggagc gcgccggcac tgacgtcgag
    19981 cgctatacgc gccacaaaga ggcgctgctg aacgtcgaac agttcaggta cggcatcggc
    20041 gccgactacg gcgggggcgt gcttggccac ggcgacattc ttcagctggt cccgacgatg
    20101 atggtggaga ggccgctgtt cgcacgcaca gtcgccgccc gctttccgta cgtcttcgtg
    20161 gacgagagcc aagacacgac ctcggccgtc gtggatgggc taaaggccgt cgcgaaggcg
    20221 gccgatggca agatgtcgct cggtttcttc ggcgacccga tgcagcggat ttatcccgag
    20281 gggatcggtg acatcgagct cgacaacggg tgggctcgcg tggagaagcc cgagaacttc
    20341 cggtcgtccg aacgagtgct tcgcgtcgtc aatgcagtta gggccgacgc ggacggactg
    20401 cagcaagtct ccggcctccc ccaggagcag cgtcgcaagg gagaggcatg gttcttcgtc
    20461 ctgcccgctg acgaccaccg ccaggggtcg ctggcgcgtg caacggaatg gcttacgaag
    20521 aacagcaacg caggcgactg gtccgcggag tctgcgcgcg gcgccaaggt cttgatgatc
    20581 cttcatgaga tggcggcccg gcgtctgggg tttcactcgc tctgggcggc cttccaccaa
    20641 gccgggcccg gatcgtcgct cgaccgagcg ttcgacgagg gggcggcctg gccgctgcgg
    20701 cccttccaac aggcgatctt gccgatcgtt agccaagtcg aaagcggggg acataccgtg
    20761 gccgcgatcc gcgctcacgc accaggcctc tcggaggaag cactcgcgga gaccgatcct
    20821 cggcacgcgc tgagcgccat tcgcgacggc atcagcgcgc tgcgggagct gatggagacc
    20881 ggtgggccag gctcggtggc gcaggcgctt agagtggcag ccgaatccgg cctcgtcgac
    20941 ctcgatccgc ggctgtcggc acaccttgag ctgccgggtg gtcccggggc cggcggtgag
    21001 atcgaggagg ccgaccagcc ggtgctggac gcgttcatgc agtgcgacgt gcggcagctc
    21061 gacgcgtact tcgactatgt cgcgacccgt acgccgttct cgacgcaaca tggcaccaag
    21121 ggcgccgagt acgaccgggt gctgctggtg ctcgatgacg acgagggatc gaagttcacg
    21181 ctctactcgt acgagaagct gctcgggctg aagccgctct cctcggctga ccagaagaat
    21241 cacaacgaag ggaaggactc cgttatagag cgcacgcgac ggctgctcta cgtcagcgtg
    21301 tcacgcgcca aggaggcgct cgctgtcgtg ctgttcgctt cagacgtcga cgcagcggcc
    21361 gaggcgctcg agcgatcggg ccttccgggg gacgctcagg ttcttaagct ccccgacatc
    21421 agctgacccg ggatacgttg ctcggtaccg ctgcatgagc caaacgctgt agtcagcgcc
    21481 taccgcttcc gcctgatttc ggcaatgcga tgagcgccac gcagcgctgc ccccttcatg
    21541 caaggccaga gctcgccggc acaccgcgtc tttggacacg accctaggcg cgacgtgcgt
    21601 agcccggccg acggcgacag tcagtcttgc tcgtcgagga cgaactctca gcgggactac
    21661 tgatccgggc gagggtcctt cagcggagtg tccctcttga gtttgctgaa cgccgcttcg
    21721 acgagcggcg acggatcgtg gccggagcgc gtggcgaagc gcaacacgcc gtgcagggtc
    21781 aagttcgccg ccgccgtcac tgagcgggtg atttccatcg ggtccggctg aagtgcggcg
    21841 cggaagtacc ccggttcgta ctgctcgcca acactggctg tgtagccgac gtgctggaac
    21901 cagttcttgc catgggcggc cgcgctggtg cttcgccaga ggaactctgc gtcgcccggg
    21961 gtcaggccga tcgcgtcggc ggtctcacgg accatcgacg agtacttcgg ctcgtgcttc
    22021 ttgatcctcg cgaggcgttc ggcgtcgccg aggcgttcgg cgaggacggt catgcgctcg
    22081 cgcgccaccg ttgagcggtc atcgccgacg gcctcgaacg cgagcgccat ctggcgtagg
    22141 tcgtggtaga gcaagcgaag gtgccggtcg acgcgctctg gcgaggcgtc gtgatcgagg
    22201 acccagacgg cctgcgccgc acattcgagg ccactgcgcg ccaacgtgta gtacgcacgt
    22261 ggcggattgc tgaccgtcat gccgtcatac accgtctgtg gggcgacgac gttcgcccaa
    22321 aagacgaggt ggtcgaccgc ggcgaccaag ttcgagcgca cccacgccga gcacttctcc
    22381 cacgggtacg ctttgtcgtc cgcgtccagt gacgacccgg ctagagggtc gtggacttgg
    22441 tggagcacgt gagacgccag ccgggacctg tgggccgcct cgacccagcg acggacctct
    22501 gcttcgggca cgcgaacaac gtcactgcac acagcctgga ccataggcag acggtcaccc
    22561 ccgcccaccc cgctgtccca cgaatgcggt tcggcgggcg cggctcaggc acgggcgctc
    22621 gatctacaaa gtaccccagc tgagccgcac tacggatgtc gaagtacgca ctggaagagc
    22681 ctcgacttcg ccaggcgacc cggcgaggaa gccgatcgcc tctcgcggta ctcgacgaca
    22741 aactcctccc gccgctcgcg cgcaacccgc gagacttcgc cgagaagacg ctcaggtcca
    22801 tacgtagcgc cgcttcggct tcctcgtcgg ccgacggttc ggggcacatg cgggatgagg
    22861 ccgacagtcc ttaggccggt atccggctcg ggttcatcaa cgagacgtgg aggctcgacc
    22921 gtggcgaagg cgccgtggag ccgccacacc agctcttggc acacaacgtt gctcccgtgc
    22981 cttcgccttc ggctgcaatc agccgcggct ccacgcgctt cctggcgtcc gccgctgccg
    23041 ctttccatgc agtgcagcgg cgcgaggtgc cacgtgcggg gaatgctgtc tcgcatggaa
    23101 cgcagcagcg ctcccttgcg atcaaacctc cggcgtcgag tcgcgaccgg caaggagcat
    23161 tcacagcggg tcaacccgcc accgctggcc acaccttgtc ggtgcggctc atcagctagc
    23221 ttccgcgcag cgtgactccc gcggagctcc gcgagcggac ggtggcgggt cgtgccgtcg
    23281 ccgatgcaga gagagcactc acggttacgc agccgactac gcacccgccg cgttcaacgg
    23341 tgatggggcg tcccgcgttg tgccgcacgg ctctgtacga ctatgggtcg cctggaagcc
    23401 agagcataca ttcgggttcg ccccgccgga cggcacgtat ccacgccccg aggacatccc
    23461 ggttgcgaag gattaaccgt tcgtggctga cggcttgcca tccgattgca gggccggtga
    23521 agtaagaact ggttacgacc attgccttgt cggcctgcgt tagccccatt accccgaagg
    23581 tctgctgaac gatcgggctt ccgactcggt ttgacgggtc ccagtgtttg cactcgacga
    23641 cgtagaaatt ccgctgcccg cggtcgacgc gcgtcccgta gatgtccacc ccaccgtcgc
    23701 gcgactgctg ggtcagctgc aagtcataga agcccgtgcg gcgaagtagc tcatgacaga
    23761 gctcctcgaa ctgccgcggg tttacaagtt cgtgcagctc cgagcggtga tcagagaggt
    23821 aatcgaggac gcgagtgccg atgaacgtga cagagccaac cgagctttgg acgcggtcgc
    23881 gtgccgatgg cgttaggact tcctcggcct gggcgcgtcc tagggccgcg tccaggacat
    23941 cgcgaaactg ttcctcgtta ggaatgacat gctggccgtc atcagccaga gctcctcgaa
    24001 ctcctgacaa gaacggttca ctcgctgccg ctacccgcga acgagagacg atgggtcggc
    24061 gcccgagcag gatttggccg ttaaggagcg ccatccgctc gcgggcctct tgctccgcac
    24121 cttcgccgaa gccctcaacg ggctcattgt gcgcgtctat gagtgcgccg tcaggaacgt
    24181 cgatcgctcc gaagcctctc gaacggccgg tctcccggtc ggtctggacc atcgcagcat
    24241 tgacctcgac taccttgccg acaaaggcgg cgagatccga tgcggtgact tcgtaggcga
    24301 ggcttccgac gaagaattcc atgcgaggcc agagcctaga aaacggcgca ccatgctcga
    24361 tgccgaccca tcgcgatcgt tgccatgctt gcgtgtcatt tggagaacga gttgcgcatg
    24421 gactatgaat ggctggcggc ctcacacggg ccagcggctg agacttggca cacgtttgtg
    24481 cgcaccgcct tggtcggccc ttggctcgat tcctacgccc agcgcactcc gtgggagatc
    24541 gcggtcatgg agatcgagct gggcgcgctt acttatctgt ttgatacggc acctacgacc
    24601 gtcggctgtg gcgatgaccg ggtcgtcgct gtctggggcc gttctcgacc cgccgatacc
    24661 ccacgggacg ctagccgtct caaggggttc cttctcgacc cggcgatttg gtcgcaagcc
    24721 ggtcgtggcc tgggtcattt cgtgggtcat gctgcaggcg gcaacatgga catgaacctc
    24781 tttccgcagg ccatcggcct gaaccgcggc acctcaaagc ggggaagggt ttggaggtcg
    24841 atggagcgct acgtcgcgcg gcatcccggc accccgctgt tcgtgcggcc gacgtacggc
    24901 gatcagacgt ggatcccggt catgatcgac tacggcctgc tggtcgatgg caccctgtgg
    24961 tccgatcggt tcgaaaacca cgactggtcg gagtccacgg gcatcccata cgactgctga
    25021 tttggacgac agagagcggc gaagggcgcc ggccgcggcg ctgctccttt gcgcgaggaa
    25081 cgggagcggc gccgggacgt cggccgggta agaccggcgc gcgaggacac gactgctgct
    25141 tcacctgagg cgggcgcccg tcgctccgtt gctttctcgc tatttccgaa tccgaaagct
    25201 agcccgcgac aagccgtgct catgtaatca cgcgcagcgg ttctgccctt agggcaaccg
    25261 aggagcgaac tggagcggat ctgccgcacc ctgagcagcc aaggagcccg gagtccaccg
    25321 aaacacgcaa ttagtcatcg ctcgtgccct ttgggggcgg cgttgcgtcc atcatctcac
    25381 cgatcttcac cgtcaatcgc gtctggaggt cctctgcgtc gtacgggtca tagccgatga
    25441 gtggatagga ccggaaatcg aagggaatgt ccttgtcttg aaaatgccgc ttgtggcaca
    25501 acaacagtat tggcttcgaa attcctaccg ccagtccgat ctcatagata acgtttgtat
    25561 tccagcctgt cacgtccgcg attacaaacc gagagcgcgc gatgccctcc atgatgcgat
    25621 ccatgatgtg ttcgaactcc gctcgggccg gcatctcgtc cgccctgatg ggagtgaggc
    25681 cagcttcaat gatcggccca cagatgttgg ccctataaaa cttggcgtaa acgtcgtcga
    25741 aggggaccgc catgaacacg ttcgtgatat tcaggctcca ggagtcgccg cgtcgcatga
    25801 aatagggttc ccgtgagagg cggcaccgcg atgcgccaag cgcggactat cgcttccaat
    25861 gcgcggcacg ctgccgccag cgaggcccgc cgctgttgct tcgcgcgaga agcagcagct
    25921 ggcgcggagg ttcgaacacc cgcgaacgac cttccggatg ggccgggctg gattcgaacc
    25981 agcgacctac ggattatgag tccgctgctc taaccgctga gctaccggcc cccgcgtggc
    26041 gaagctagca ccgcgtacgc gaccgggcac cgccgggtcc accgggcacc gccccccctc
    26101 agcgcccgct ggtcgcctcc cgcagcgcct cctcggcctc ctccgcgctg aacgcaccct
    26161 cgatccgcgc cgccacgcgc ccctgtgcgt cgatcgcgaa gagccacggc tccgacggca
    26221 gcccgaaggc gcgcacctgc gggcgctcgc cgccgtcgcg gctgttgccg ttccagacct
    26281 ccatgtggat gtaggccgcg tcgtcggggt ggtcggcctt cacctgctcg agcacgtcca
    26341 ccaccggacc gcagatgcgg ctgcgacaca gccgcggcgt tgcgaacagc agcatcaccg
    26401 gccggtggcc gagcacgtcg gccaggtcgt cctcgtgcat cgtgctcggc ggctcgcggg
    26461 tgtcgatctc ggagaggtcc ctcacctcgt cgcgggtggg agtgttaacg cgcggcgccg
    26521 cgtccccgac ggccggcggc gcggtcgggc gctcgacccg cacgcgcacc ggcgtcccac
    26581 cgaccacgcg ctggtcgagc tgagtcaccc caagcacctc gtaattgccc tcgcgcttga
    26641 acgggatgtc ggccacgtag atcgagtgtg cggcgtcgtg gtcgtgcttg acgctgtcgc
    26701 tgcgatgcgg ttcctccacc tccagcgacc ggtaccgcgc cgggtacggc ccctcgacca
    26761 cggccccgcc ctcgggagcc acgtagacag cagcgggcgt gtcggcgatc tggcgccggg
    26821 tgcggtcgaa cagcgcgaac gcgaagcggt tgcgccccgg ctggagcacg gagaccgact
    26881 tcgccatgag cggcccgggc ccgagaccgg cgcgcagctc gtcgagcgtc cgcgagctgg
    26941 ggtccggaaa cggctgcgcc ctcccgaccg gctggtcgga acccgacggg gacccgccgt
    27001 cgtcgccacc gcctccgcac gcgccgagcg ccaacgccgc aaccgcgcac gcggcagcgc
    27061 acccgcgaag agcccctgca cgcaccatgc ctcagagcgt acggaaagcg ggcaggcgcc
    27121 acggcacacc tgcgcacaca gcgtgtcggg tagaaacccg ccctcacgaa cgaagacgga
    27181 ggacgaccga cgatgaaagg ccgtgcagct ctcataaccg gaggctcgag cgggatcggg
    27241 ctggcaatag cccgcgcgct ggggcaggac gggtactcgc tgacggtctc ggcgcggcgt
    27301 cccgagaagc tcgaggaggc ggtggagggc ctccgcggcg aggggctcga agtcgaatgc
    27361 gtgcccgcga acatggccaa cgaggacgag atcaagggcc tcatcgcccg ccaccgcaag
    27421 cgcttcggcc gcatggacgt gctgatcaac aacgccggcg tgggcatcgg cggcccgatc
    27481 gccgagcagc agaccaagca cctcgacatg cagatcaacg tgaacctgcg cggcgtctac
    27541 ctgaccctgc gcgagtcgat cccgctcctg aaggaggccg gtgccgagca caagaaggcg
    27601 ctcgtggtga acaccgcgtc gatcgccggc aagttcgggc aggcctggct ggggacctac
    27661 tcggccacca agcacgcggt cgtcggcctg tcgcaggccg cgcacggcga gctggccgag
    27721 tcaggcatcc aggtgaccgc gttctgcccc ggcttcgtgg acacgccgat gaccggctgg
    27781 gtcgagggcg tcccgcccga ggcgatgatc cagccggccg acctcggtga ggcggtgcgc
    27841 ttcctgctgc gcacctcgcc cgcgtgcatc gtgccggaga tccagttcat ccggcccggc
    27901 gagacgcagg tgaagaccta gctagacgag ctcgcgctca gggcgctcgt cgcgctcggc
    27961 ggcctcggcc gcctggcgct cggccgcggc gaccgcggcc tcgtactcgt cgaggcggcg
    28021 gaacacccgc agccaggacc agcccgggcg gcggcgcagc tcgtcgctgc gatcgcgctc
    28081 gccggtgtcg gtgcgctcgc gctcggcgct ctcgccggcg agcttgcggt gctcttcggc
    28141 cgcctcggcg aggtcggcgg cgttgtcctc gtagtagagg ctcgcctcga gcatggcgcg
    28201 cagcgtgcgc agctcccgtt cgctggcctc gcgcatggtg gtcgcgtgcg tgtaggcgtc
    28261 ccaggcgtgg gcgaagctgg cgaggttcgt gcgcccgccg ttgcggtgcg ccgcgagcat
    28321 cgggcagatg ccgccgtcgc cgtccgtgta ggcgccgacg atgatccgct cgtcgtcgag
    28381 cgactcgagc atcgcgaccc gcgttccgcg cggaaggcag tcgactgccc ggcgcagatc
    28441 ctctgcatgc atgcgatgtc ggcgtcgtct catcaggtga gtcgtccgcg gtcgcttagg
    28501 atgcgccccc ggcccgcacg gggaatgtac ccgtctcccg tccggatatt caagcggatt
    28561 ccttccgact gagaggaagt atgcagatgc gacttgggtt gcagctcggc tactggggcc
    28621 tgggcatcag tgagcaggac cagctcaaga tggtcaggga ggccgaggcg gccggctacg
    28681 aatccgcctg gggagccgag gcgtacgggt ccgataccgc cacggtgctg gcctggctcg
    28741 ccggccagac cgagaagatc aagctcggcg cgggcatctt ccagatgccg gcccgctcgc
    28801 ccgcgatgac ggcgatgacc gcggccacgc tcgacaacct ctcgaacgga cggatgctgc
    28861 tcgggatcgg ctcgtccggg ccggtggtcg ccgagggctg gcacggccag ccgttcgcca
    28921 agcagatcca gcgcactcgc gagtacgtcg agatcgtgcg caaggcgctc acgcgcgagc
    28981 ggctcgagta cgacggcgag acctacaagc tcccgctccc gggcgcccag ggcaagccgc
    29041 tcaagctgat gatcgcgccg atccaggaga ggatcccgat ctatctggcc gcgatcgggc
    29101 cgaacaacac gaagctggcc ggggagatcg ccgacggctg gctgccgttc ctgttctcac
    29161 ccgagcacgt ggacgacggg cgccggctgc tccaggaggg cgccgaccgc gtcgggcgca
    29221 ccatcgacga ctcgttcgac atcgcgccga acgtgaacct ctgcatcgac gacgacatcg
    29281 agaaggcgcg cgacatgctc cggccgatcc ttgcgctgta tatcggcggg atgggatcgc
    29341 gcgagcgaaa cttctacaac caggcggtgc agcgctacgg gttcgaggag gccgccgcca
    29401 aggtgcagga cctctacctc gaccagaagc gcgaggaggc gatggccgcg ctgccggcgg
    29461 agctgatcga cacggtcgct ctggtgggac cgaaggacag ggtgcgcgac cggctggccg
    29521 cctaccggga cgccggcgtc gggacgatgg tcatcacccc gctcaccgag cccgagaacc
    29581 gcgcgcgcat gctgacggac ctggccgagc tggtctgagc accgcccgcc gcatcgctct
    29641 cgccgcgttc ggcgaccccg gtcacgtgtt ccccgtgatc gcgctcggct ccgagctcgt
    29701 tcggcgcggg cacgaggtcc aggtcgagac gtggtcgaag tggcgcacgg acgtcgaggc
    29761 cgagggaatg cgctttgccg cctcgccgga gtacgaggtc ttcccgcccg acggccggat
    29821 gctcaagccg taccaggccg cggtgcgcgc ggcgaaggag accgtgccgg tgctccgcgc
    29881 gttcgacccc gacgtggtcg tcgtggacat cctgaccgcc gcctcctcgc tcgccgccga
    29941 gatggacggc cgaccgtggg cgacgctcgt cccccacgtc ctccccactc ccgggcccgg
    30001 cttccccccg tactcgatcg gcgcgcggct gccgcgcacg gccgccggcc gggcgctctg
    30061 gcgctcgctc gacccgttcg tgcagtccgg cctgcgacag ggccgcgagc agctcaacgg
    30121 cgcgcgcgag cgagtcgggc tggcgccact gaaccacctg cacaacggga tctcgcggcg
    30181 gctggcgatc gtggccacgt tcccgcacct cgagtacccg cgcccgcggg cggagccgtg
    30241 ggtggagacg accgggccgc tcctctggga gcagccgttc ggcgacgtgg agctgccgcc
    30301 gggcgacgct ccgctcgtcc tgatcgcgcc cagcacctcg caggatcccg agcagcggat
    30361 gctggccgcg tcgctggcgg ggctggccga cgcgcccgtg cgcgtgctcg ccacctacaa
    30421 ccggcggccg cctcccgagc cgctcgacgt gcccgcgaac gcgcggctcg tcgaatgggt
    30481 ctcgtacgcg cgcacgatgc cgctctgctc ggcggtggtg tgccacgcgg ggcacgggac
    30541 ggtggtgcgc gcgctgtcgt gcggcgtgcc ggtggtggcg tgcccggccg cgggcgacat
    30601 ggcggagaac gccgcgcggg tgagctgggc gggcgtgggc gtgtcgctgc cgcggcggct
    30661 gatcacgccg cgcggggtgc ggctggccgt ggagcgcgcg atcggtgagg agcgcatcgc
    30721 cgcccgcgcg cgcgagctgg cggcgtggtc gcgcgagcac gacgggcggg tgcgcgcggc
    30781 acgggcggtc gaggccttcg ccgcacgcga ggcgatcggc cgggcgtagc cttaccggtg
    30841 cgttgaccga cgatccggac acgcgcgacc gccgcgacga cgagcgccgc aacgacgagg
    30901 agaacgagcg cctcgaccgc gagctgatgg aactcgtcaa cgagctgcgc gtggcgctca
    30961 cgggcgtgca gatcctcttc gccttcctgc tcacggtccc gttcacgcag cgcttcgcga
    31021 cggtcaccga gttccagcgc cacctcttct tcgtcacgct gctggccgcg gcgctcgcgg
    31081 tggtgctgct gatcgcgccg accgcgcgcc atcgcctgct gttccgccag cacgacaagg
    31141 agcgaatcgt ctccagctcg aaccgcttcg cgatagccgg cctcgcggcg ctcgcagcgg
    31201 cgatgaccgg agcggtgatg ctgatcaccg acctgctctt caaggagacg atggtctcct
    31261 gggtgaccgc gatactggcc gcgctgttcc tggtgatgtg gttcgtgatg ccggcgctgc
    31321 ggcgcgtggc gcagggccgc ggcagctagc gggcccaagc cacggaaaca actgcctcgc
    31381 ggcggcgttc tcgaagattg gaacgttcgg tatctatggg ctgcaggggt cggcacctcg
    31441 atctagatct gcggttggag gtccgccatt ccgccatgag ctgtcctgtc gccagcgatg
    31501 tgtctctcag gccgcttctt cgagcggatc gctgcggagc atgtaggcgc atcggtcgaa
    31561 gaccacgtct accgcgtggg tctgccagcg ttccacgtcg cgcagttcgc gacggcagtg
    31621 ctctgggttg tcgcagtgat tgcgtcgcgc atggcggctg acgacagcgc cggttcccgg
    31681 ttgagtgcgc tctgggagac cggcgccgca ggcgatgcac tttccggcgc cccgccaggc
    31741 aatggcctgt gacccggcgg ctatgaggtc ccggtactgc gcggtcggac gacgccattg
    31801 gtcgtcttcc gaaaggggac tctcggggaa gtacgcgtcg ctgccgagtt gcagaaggcg
    31861 tatctcggcg agcgttgaac tcgcgccttc gtcgagcgac cgtgctcctt cacggaggta
    31921 tagcgcgatg atgcgacggc gggtggggcc gttgccgtaa tacccggcga tgtgcagcgc
    31981 aaggtcggac gtgtcgggtt cttcgcgttg ccagcgccca gagcatcggc tgtataggtc
    32041 cgcgaagcgg cggtaggccg gatcaagctg gacgtggcgc gcgtggtccc agaagccggg
    32101 ctcggtgccg agggcatcgc ccagagctgc tgggaccatc atgcgcttaa gcatcgccag
    32161 gtctttgcgg ttgagatctc gcaaggttcc tccaggggtc gatgacgccg aagagcatgc
    32221 gctcggtcgc ctgaggaaaa atccaacctt gttctcagcg cgccgagcgc tggtcagcag
    32281 tctgagcaag aggtcggcca gcaagaccac ctttcaggca agcgttgggt cgcttgtatg
    32341 cggcctgggc ggtgactggg ttctcgccca caccgatggc gtcgccgtac acggccatca
    32401 acgcctcagt tcgcggcaac cgttcaacgc cggccgggct gcctagccgc cctcagtggg
    32461 tcagctcgct tcaatcaact catgcctgcc ggcaactcca aggccgacaa cgcgttcaac
    32521 cgggcggagc agtggctttc gcaacgggcc gacgagcacc tgccgaccct cgacctggcc
    32581 gaccgcagct ccgatgcgct cgggcgcgtt ctccaagcat tcaatctgcc gaagcgcacc
    32641 cctgagcagc gggacgacga gtggcgtcaa acgctcgctg tgatcaacct ggggatcaat
    32701 gccgctagaa gcctccgctc gatgatggtc ctactgcgac acggctacga gccggaggcc
    32761 atcgcgcttc agcgtcgact catcgaagcg caagcacgaa tcctccgtgt catcgacccc
    32821 gtcagcgggc cagaccacgc gcggcaattc ctcaagggcg aggacggcac cgccggcaag
    32881 gcggtcagcg cgcttccgaa agacttgtgg aaagggctca ataacgcggc ccacgccgac
    32941 taccgcgcca tcgagcagca cctgctgtca accgatgagg agggcagcac gaggttctat
    33001 ctggtgccgc ggcggcgcga gaccgcgtcg aacatgctcc tcgtcctcgg cgccgggcat
    33061 gtgttgtcgg cggcggccga gatcggcaaa ttcaaggcga tcgcactcga tcctctcatg
    33121 gatgttcttc cggacttcta ctcggcgact gaacgctggc tgaccccgcc tgagacggac
    33181 acctgacgcg aaggccgcgt ccgtgcgcgg agcgaccctc gcggtgctga ggaagggcgg
    33241 gcaagccgcc ggcacgtaag acgcgactcc gaaaacaagt tcgccggccc cgaaggaccg
    33301 gcgaacgaac gttccggaac cttgcggaag gtgccgaacg cttgaaagct gcgggggtgg
    33361 gactcgaacc cacaaccatc gccttaacag ggcgccgctc tgccgattga gctaccccgc
    33421 aacgagggcc gacaggttag cggctccccc ctcgatctcg gcgtggcagt tcgcgcacac
    33481 gagcacgcac tttcgagcct cctcgcgcgc ccgctcgagc gaccgggtgt ggccacgacg
    33541 ggcgatcgcg aacgacttct cggacgggtc cacgtggtgg aagtgcagcg cggcgacgca
    33601 gcggtcgtac ccacatagga cgcagcgtcc acccgcctcc tgcacaagca gctccttgat
    33661 ctggcggcgc cgccgcgcga cgcgctccat ccggcagcgc gtgcacctgt agtagccccg
    33721 gccctcgagg acgaagtcga cctcaccgtg agtgcgacac tcgcgcttta cacggcgaag
    33781 cgaccgttcc atgaccggaa ggtacggtct ggatcggacg gaagagacga acgtacgttc
    33841 gtccctggaa cccctactgc tcgatgtctt ccttgagctt ctcggcctcg cgcgagttct
    33901 tcagcttcga gagcgtcttc gactcgatct ggcgcacgcg ctcgcgcgtc acgccgacct
    33961 cggtgccgat cgcctcgagc gtcatcggct cgtccgacgc caggccgtag cgcagctcga
    34021 gcacgcggcg ctcgcgccac tggagcgact tcagcgcggc ctcgaggtcg cgctggcgca
    34081 ttccctcgga cacgacctcc aggggcgcct cggcctcgtc gccctcgatg aagtccgcca
    34141 gccgcgactc gccctcggcg ggtccgaccg gtgtctcgag cgacaccggc tcgcgaccga
    34201 gctcgagaat ctcctcgacc tgcgtgggcg tcatctccac ctcgggagcg atctcctcgg
    34261 gggtcggctc gcggccgagc ttctggttca gcgcgcggcg cgcgcgaccc acgcggttca
    34321 tccgctcgac catgtggacc gggatccgga tcgtgcgcga ctggtctgcg agcgcgcggg
    34381 tgatgccctg gcggatccac cacgtcgcgt acgtcgagaa cttgaagcct cgccgccagt
    34441 cgaacttctc gaccgcgcgg atcagcccca ggttgccctc ctggatcagg tcgagcagcg
    34501 tcaggccgcg cccggtgtag cgcttggcga tcgacacgac gaggcgcagg ttcgcctcga
    34561 tcagctggtt cttggcctcc atgtcgttgg cctcgacgcg cttggccagg cggacctcgt
    34621 cctccttggt cagcaggcgc accttgccga tcgacttcag gtagttgcgg accgcgtcgt
    34681 cggcgccgcc ctcaggctgt gcggcaggga cgccggccgt aacgaccccg ccctcgtcga
    34741 acaggtccgg caccttggcc gtccgcggct cccgctcggg cgcctccggg tcgtccagcc
    34801 gtgccgagtt caggtcgttc ataccgcctg tcccatcagc tcgttgatct ggtccttgat
    34861 tcgcatctgc tccgggccga gttcacgcaa tcgtcccaga tcgccgtcgc gctgtgcgcg
    34921 gcggatctgg cgctgcacgc gatcgagcgc gagctgctcg aaagccagcg cgagcgagcg
    34981 cgggtccccg tcctgctcgc gcatcgccac gtcggtgatg agcgcggtga gcggcgggtc
    35041 gtcgatcggg agatcggcga gggggtcgtc agcgtgcttg acgaggtgcg cgcggccacg
    35101 gcgcagcacg tccgaggaga agtagtcggc gtcgactcgc tcgaggtact cgcgcccgag
    35161 ctccccggcg gcgacgcaca tggcgaggaa gctgcgctcg cgacgctcga aggcgtcggt
    35221 gggcagcgga gcggcggccg gctcggcctc gcgcgcgacg gggcgcgggc gctgcggagc
    35281 ggcgacctgg gaggtgacgt aatcggaggg cacatccaac ctgtcggcaa gaaagcccac
    35341 gaggtcatcc cttgttgcgg aatttgcggg cagcgccgcg acgagtgggc gcacctgctc
    35401 gagcgcgcgg tcgcgcccgc ggggcgatcc gaggtcggcc tgcgccacca cccggcgcgc
    35461 ctcaaagtcg ggaacgaggc gcgctgagtc gatccggcgc gcgaactcgc cggagccctc
    35521 gcccgcgatc aggtcggccg gatcggcgcc ctcgggcatg tcgacgacgc gcagctcgag
    35581 gtcgaggtcg cgcgcgccac gcgcaccgcg gcgcatggcg tcctggccgg cgcggtcggc
    35641 gtcgagcgcc aggtagaccg ttccgtcctt accgacgcgc tgcgccagtg tcgtcagctg
    35701 ctgctgcgtc agggcagttc ccatgatcgc cacgctttcg gttatccccg cctggtgcag
    35761 cgcaagcaca tccgtgtacc cctcaacggc aacaatcctt cccctcttgg ccgccgtgga
    35821 cgctgcgcgg tcgatgccga acagctggcg gcccttatgg tagagctcgt tttcggacgt
    35881 gttgagatat ttcggccgct gatcctctct caatgagcgc gcgccgaatc ccagcacccg
    35941 gccgcggtag tccgccagcg ggaacatgat gcgcccgcgg aagcggtcgt agaccccgcc
    36001 gcgcgtgccc cgctgcgcca gtccggcggc cgccagctcc tcctgactga agccgtcccg
    36061 ctgtgcagcg gtcaaaagct tgtcccatgc gctcggggaa tagccgatcc ggaactcgcg
    36121 caggatcgcc tcgccgaacc cgcgtcctgc taggtagtcg cgcgcggtgc gcgcctcgtc
    36181 ggtctcccaa agatggcgca cgtagaacgc cgtcgcgcgc tccacgagcg actggagccg
    36241 ctcgagccgc ttgcggcgct cctcggcctg cgggtcctcc tgctcgcgct tgagctctac
    36301 ccggtagcgg tcggccaggt actcgaccga ctccttgaag tcgagcgcct cggtggactc
    36361 gacgaaccgg atcgcgtccc cgccctcgcc gcagccgaag cagtggtaga gcttgtgctc
    36421 ggcgtccacc gtgaacgacg gggtgcgctc gtcgtggaac gggcagagcc cgctccactt
    36481 gctcccgacc cgcttcaggt cggtgcgcgc ccccaccagc tcgaccatgt ccacggcttc
    36541 cttgacgcgt tcgatcgagt ccttggtgta gagcgccatg ggtcctggag gctagtcgcc
    36601 gagggctgct tcgagggcct tggtcacctc tccgagcgag acctcgtccg cctccagctt
    36661 ccggaacgtt gcggccgagc ccgcccggag gatcacgagc gtgggcgtgc cctccacccc
    36721 ggcccgctcg ccctcggcct tcgcggcgtc gatctgctgc gtcggctcca tcccgctgcg
    36781 cgcagcaagg gccttcggca catcgagccc ggcagcccgg ccgatccggc ccaggtactc
    36841 gtcgtccacc cagccgtcgt tctcggcgcc ctggttgcga tagatcaggt ccaccacctg
    36901 gaagagccgc ccctgcatcc ccgcggccgc cgcgaagcgc atcgcctcgg tggagtcggg
    36961 cccgatgaag tcgaggccgc ggaagttgat cctgaccttc cccggccgca cgtagcgcct
    37021 gaccacctcc ggcatcacct cgcggctgta ctcgccgcag aagggacact ggaggtcggc
    37081 gaactccgcg agcgttacct tcgcgcccga gtcgccgagc agtgggcccc gctgcggtat
    37141 cccgcggtag agagcctgca cctcggccac gccctcgggc ggcccggtcg tggtccccgt
    37201 gcccccgccc gtccggctca ccccgatccc gatcccgatc aggatcgcca cgagcagcgc
    37261 gctcgccccg aacagccaca gcctgcgacg gcggcgctcg gcgagtgcgg ccgcgtccgt
    37321 cacgaattct ctgcggacgc tccctcggca gccgccgcct gctcgcgctt gcgccgccgg
    37381 cggtcggtga acagcacgac ggcgatgaac gacaggaacg ggccgaggta gatcagcaag
    37441 tcgatgaccc agtgtccgcc ggcgccatga gcgagataga ccattgtttt cacaatacgc
    37501 gcgcggtcgc cctcgggatc cctagaaccc ctgcggcacc gacagatcgc tgaacacgcg
    37561 caccgcgaag cggtcggtca tccccgcgag gtagtccacg acgcgctccg cctcgctcgc
    37621 acccgcgacc agcgccggcg gcgggttctc ggcgtagtgc gcgaacagcg tgcgcagcat
    37681 ccgctcgatc cgcgggtgct cgctgcgggc ggtcgccccg aggtagacgc ggtcgaacat
    37741 gaacgcgcgc aggcggtcca tcgccgcgcc gacctccgga ccctggacga tcgccccggc
    37801 gtccgccgag cgctccacga ggtcgcgcac cagcgtgtcg atccgccgcg acccggtctc
    37861 gccgaggacc tcgatctcct cgaccggcag ctcctcgcgc acgatcaccc cggcgcggat
    37921 cgcgtcgtcg atgtcgtggt tgatgtaggc gatgcggtcc accagccgca ctatctggcc
    37981 ttccagcgtg gccggcttct cggggcccgt gtggcgcagg atcccgtcgc gtacctgctc
    38041 ggtgaggttg agcccggcgc cgtcgcgctc gatccgctcg accacgcgca gcgagtggtg
    38101 gttgtggcgg aagccgaacc cgtagcgctc gcgcaggcat gcgtcgacca cgctctcccc
    38161 cacgtgcccg aacggcgggt ggccgaggtc gtgcccgagc gcgatcgcct cggtcaggtc
    38221 ctcgttcagc gcgagcgcgc gcgcgaccgt gcgcgcgatg ccggtggcct cgagcgtgtg
    38281 ggtgaggcgc gtgcggtagt ggtcgccctc gggcgagatg aacacctgcg tcttgtgctt
    38341 gagccgacgg aacgccttgg agtgcacgat gcggtcgcga tcgcgctgga acggcgtgcg
    38401 catcggcgag tcctcctccg ccaccgcgcg gcgcgccggg tacgagcgct cggcccgcgg
    38461 cgagaggttg gcgtcctccc aggctcggag gcgatcgtgg aactcgggtg tggagacgac
    38521 gggcacggtc ctgattgtct cagggccggc ggcggcggat ctagcccccg cgcgcgatgc
    38581 tcgcctgcgc ggccgccacg cgggcgatcg gcacgcggta cggcgagcag ctcacgtagt
    38641 cgagcccagc catgtggaag aagtcgatcg actccgggtc gccgccgtgc tcgccgcaga
    38701 tgccgagctt caggttcggc tttgcctcgc ggcccgtcca cgccgccagc cgcacgagcc
    38761 agccgacgcc cggcttgtcg atcgtctcga acgggctgcg atcgacgatc cgctcctcga
    38821 gatagcgcga gaggaagccg ccctcgacgt cgtcgcgcga aaagcccagc gcggtctggg
    38881 tcaggtcgtt ggtgccgaac gagaagaagt cggccacgtc cgcgatccgg tcggccacga
    38941 agcacgcgcg cggtagctcg atcatcgtcc ccaccgtcag gtcgagcccc tccccgccct
    39001 cctcgctcac cacgcgctcg acgagcttgc gcatgatctc gagctcggcc tcgtaggcga
    39061 ccagcgggat catcagctcg agccgcggcg cgtccccgct gcgctcgcgc acggccttcg
    39121 cggcgcggac gatcgcgtgc acctgcatcg cgtagatctc cgggtgcacg atcccgagcc
    39181 ggcagccgcg cgtgccaagc atcgggttgg tctcgtgcag cacgtgcacg cggtcgagcg
    39241 tgcgctcgag ctcctcgagg tcgggcgtct gctcgatccg tgcgcgctcg acctggtggc
    39301 tcagctcctc gacgtccggg aggaactcgt ggagcggcgg gtcgagcagg cggatcgtga
    39361 ccggcaggcc ggccatcacc tcgaagatcc cctcgaagtc gcgctgctgg agcggcagca
    39421 ggtcggcgag ccagcggtcg cgctcctcgt cctcggcggc catgatcatc gcgcgcatct
    39481 tcggctggcg gtcggcggcc atgaacatgt gctcggtccg gcagaggccg atgccctcgg
    39541 cgccgaactc gcgcgcccgg cgcgcgtcct ccggcgtgtc agcgttcgtg cgcacgccga
    39601 ggcggcggac ctcgtccgac cactcgagca cgcgctcgaa gttggcgtcg acctcgggct
    39661 cgaccagcgg cacgtcgtcc acagtgatca ccccgtcggt gccgtcgatc gcaatcaggt
    39721 cgccctcgtg caggaccgtc ccgttcacgc gcgcctcgcc gctggccagg tcgatctcga
    39781 gcttggacac gccggccacg cacggccgcc ccatcccgcg cgcgacgagc gccgcgtggc
    39841 tcgccttgcc gccctcggag gtcacgatcc cgcgcgcggc ccagaagccg gccacgtcgt
    39901 cggcctcggt gaacggccgc atcagcacca ccgcgcggcc ggactccgcg gcctcgaccg
    39961 cgcgctcggc gctgaacacg atctcgccct tggcggcgcc cggcgacgcg ttgaccccgc
    40021 gcgccagcac cgtgtagtcg gcgtcgcggc cgaaggtcgg gtgcaggagc gcgtcgagcg
    40081 agttggcgtc gatggtcgcg agggcctcct cgcgggtgag gaggctttcg cccaccgcgt
    40141 cgacggcgaa ccgcacggcc gcctgcgccg ggcgcttggc gttgcgcgtc tggagcatgt
    40201 agaggcggcc ctcctccacc gtgaactcgg tgtcctgcat gtccttgtag tggccctcga
    40261 gcgtgcgcag gatgtccatt agctcggcgt gcgcggcggg catcgcgtcg cgcatctcgg
    40321 acaggtcgcg cggcgtgcgc acgcctgaca ccacgtcctc gccctgggcg ttcacgagga
    40381 agtcgccgga cggctccggc gcaccggtga tctcgtcgcg cgagaacccg acgcccgagc
    40441 acgacgtgtc gcccttgttg ccgaacacca tctgctgcac gttcaccgcg gtgccccagt
    40501 cgtcggggat ccggttgatc cgccggtact ccaccgcgcg cgccccggtc caggactcga
    40561 acaccgcgcg gatcgcgtgg cgcagctgct cgcgcgggtc ctgcgggaac tcctcgccgg
    40621 tctgcgcgcg gtagagctcc ttgaagctgt cggtcagcgc acgcaggtcg tcgacggaca
    40681 gctcggtgtc gagcttcacg ccggcgctgc gcttgcgctc ggcgatcgcc tcctcgatca
    40741 actcgcccgg cacgccgcgg cacacgttcc cgaacatctg cgcgaagcgg cggtaggagt
    40801 cccaggcgaa gcgctcgttg ccggtcgccg cggcgaggcc ctccaccgag ctgttggtga
    40861 ggcccaggtt gaggaccgtg tccatcatcc cgggcatcga ctcgcgcgcg cccgagcgaa
    40921 ccgacacgag cagcgggtcg gccgggtcgc cgagccgctt gccggctcgc tcctcgagcc
    40981 gctccagcgc gcaagacacc tgctcctcga ggtccgccgg ctccaccttg ccggcgctca
    41041 ggtaggccac gcacgcctcg gtggtgatcg tgaagccggc gggaacgcgg tcgggaccga
    41101 gcacgcgcgt catctccgcg acgttcgccc ccttgcctcc gagcaggtcg cgcatctcgc
    41161 gcgagccctc gtggaagtcg tagaccagct tgctcggggc ccccacgcgg ggcgagtcta
    41221 cgccgccacc cgccgcagcc gcacgtgcgc gtggtactcc atcgtctccg ctatcgcccg
    41281 gtccgcctgg cgcagggacg cctcggagcc ggtcgggtag tcggccagcg ggcggcccag
    41341 cgcctcggcg aggaacgcgt gcgccgcgcc gtcgagcggg aacgacccgg cctcgcatcc
    41401 ggggcagacc actccccccg cgctggccga gaacgcgctc aggtgctcgc gctcgccgca
    41461 ggaggcgcag gacgtgagct cgggcgtgaa gcccgccgcc agcagcagct tgatcctgaa
    41521 cgccagcgcc tgcgcgcggt tggccgccgc ggggtcggtg ttgaggaggt ggagctcgtg
    41581 gcagagcagg ttgtaggcgg gctggttagg ctcctgcgag tcgagcagcc gcagcacggc
    41641 ggcacacgcc tcgacggcgc ggtcgagcgc cgcccggctc tcgcgcagcg ccggataggg
    41701 gtccaccgtc tcggcagact ggaccgtgca gaggtcgccc cgaccctcgc gcaggaccag
    41761 ctcggagcgc accatcggct cgagccggcc gccgaagcgc gacttgaccc ttctcacccc
    41821 tttggcgatc gcgcctaccc ggccgcggtc ccgggtatac agatggagca cgcgatccgc
    41881 ctctccatag cggatcgagc gcaacacgat cgcctccgtc gtgaaggtgg ggggcattcc
    41941 tagactttct cgcaagtggc ggacacgacc gaaatcatcg tttacaccac ggagccgtgc
    42001 ggcttctgcc gccaggcgaa ggccctgctc cagagccgcg gcgtcgagta ccgcgagatc
    42061 aacctcgcca aggacgccga gggccgcgcc gacctggttg cccaaacggg gcagatgacc
    42121 ttcccccagg tggtggtcgg cgagcgctcg atcggcggct tccgcgagct gctgatggcc
    42181 gaccggtcgg gtcagttgca gtcgctgctg gcggcctagt tcacaaacgg gactatgtgt
    42241 cccgcttttg cactcggggc ctccgctggc accccgggca gacgtaggtc ccccgccccg
    42301 ccgcgcgcat cttcacgatc gtccggccgc agcgcacgca cggctcgccc tcgcgcagat
    42361 gcaccaggaa gcggtcctgg aagctgccct tggcgccgtc ggggttgcgg aagtcgtcga
    42421 tcgaggcgcc cttggcgtcg atcccgtcgt tcagcacgcg cacgaccgcc tcgcgcagct
    42481 cctcgatctg cgggcgcctc agcgtcccga ccggccgcag cgggtggatc ttcgcccgga
    42541 acagcgcctc gtccgcgtag atgttgccga cgccggccac gcgctcctgg ctcagcagga
    42601 acgccttgac cggctgcttg cgcccgcgcg cctgcgcccg cagcgcgtcg gcggtgaact
    42661 cggggtcgag cggctccacg cccacgcggg tcgagaagta ctcgtcgcgc gcgtcgtcgc
    42721 ccagcaggac cacgccggtg ccgaagcggc gcgggtcggt gaacagcacg cgctcggacc
    42781 cgaagtcgaa gcggacccgc gtgtacggcg cctcctcggc gacgagcagg ttgccggtca
    42841 tgcgcaggtg catgatcagg tggacctcgt cggcgagctc gaggtcgagc cacttcccgc
    42901 gccgcgtcag gcgctcggcg cggcggccgt ccaccgcggc cgcgacctcc tcgggcgggg
    42961 ccgggtcgca ccacagcggg tcgagcacct cgagcgagcg gagcacgttc ccctcgacca
    43021 cgggcgccag cgcgcggcgg atcgtctcga cttccggcag ctccggcacg gagccggaga
    43081 ctagggacta cgccgtcgcg cgcgggtgcg ccttgaagta cacgtccttc aggcgctcgc
    43141 tggagaggtg cgtgtagagc tgggtggtcg ccacgtcggc gtggccgagc atctcctgca
    43201 ccgagcgcag gtcgcagccg ccggcgagca ggtgggtcgc gaacgtgtgc cgaagcgtgt
    43261 gcgggctcat ccggtcggcg agcccagcgg ccgctgcgtg gcggcgcacg atcttgtaga
    43321 gcccctggcg ggtcatgccc tcgccgcgga agttcacgaa caggtgcgtg accggccgcc
    43381 cgcgcaccag cgccgggcgc ccgcgctcca ggtagcggcg caccgcgtcg agcgccttgg
    43441 tccccaccgg caccacgcgc tccttcgagc ccttgccccg cgcccgcagc acgccgtcct
    43501 cgaggtcgat gtcgcgcacc tcgatcccga tcgcctcgga cgcccgcagc ccgcaggcgt
    43561 acatcagctc gagcagcgcg cggtcgcgca gggccgccgg ctcggtgccc ttcggctgct
    43621 cgagcagctt gccgatctcg ccgcgcgtga gcaccttcgg cagccgccgt ccgcggcgcg
    43681 ggcccgcgat cgtggccatc ggatcggagt cgcgcacccc ctcgcggcgc aggtggcggt
    43741 agaacgagcg caggcaggcg gccttgcggt ggatggttgc gggcgaggac ggcgcccggc
    43801 cgttgccgtc gcccgcggcg agctcggcca gaaagtccga gacgtcggcg ctggaggcgt
    43861 cgtcggcgcc gcgaccgcgc tcctccagga agcggccgaa ctggagcaga tcggaccggt
    43921 atgcctcgag cgtgttgcgc gacagaccgc gctcgaactc cagatacgcc aggaagtcga
    43981 ggaccaggtg ctggtacggc ggggcctcgg ctatatgagt gattgtcgcc acacgaccct
    44041 ggttcggccg cgccaccgcg acccctcgct gtgcaacccg gctgtcaggt gggtatgtga
    44101 ctcggagacc aaactttgac gagaggggca acctgatggc cagcaacgtg agtgagcgtg
    44161 aggcgcgaga ggtcgcggag gccgcgcgcg agaccgagtg gaagctgccg agcttcggca
    44221 agggcctgtt cctgggcgac ttccggctcg acctgatcca tccgcagccg ccgctgggcg
    44281 acgaggccgt cgagaagggc gagcgcttcc tggcgcgcct gtcggcgttc ctggaggagc
    44341 acgtcgaccc gctccagatc gagcgcgacg cgcggatccc ggacgaggtg gtccagggcc
    44401 tcaaggacct cggcgccatg gggatgaagg tccccgagca gtacggcggt ctcggcctct
    44461 cgcaggtcta ctacaacaag gccctggcgc tcgtgggcag ctcgcactcg gcgatctcca
    44521 cgctgctctc ggcgcaccag tcgatcggcg tcgccgagcc gctcctcctg ttcggctccg
    44581 acgagcagaa gcagaagtgg ctgccgctgg tggcgaagga ccacgtctcc gcgttcctgc
    44641 tgaccgagcc ggacgtgggc tccgacccgg cgcgcctcgg caccatcgcg gagccgaccg
    44701 agggcggcta cctcctgaac ggcctcaagc tgtgggcgac gaacggcgcc atcgccgaca
    44761 tcgtcgtggt catggcgcag gtgccgaaga gcgagggcca tcgcggcggc atcaccgcgt
    44821 tcgtgctcga ctacaagagc gacggcgtga ccgtcgagca tcgcaacgcg ttcatgggcc
    44881 tgcgcgggat cgagaactcg gtcacgcggc tcgagaacgt gttcgtcccg gcggagaacg
    44941 tgatcggccg ggagggcatg gggctcaaga tcgccctcag caccctcaac acgggccggc
    45001 tcgcgctgcc ggcgatctgc gtcggcgcgg gcaagtgggc gaccaaggtc gctcgcgagt
    45061 ggtccaccca gcgggtgcag tggggccagc cggtcggcaa gcacgacgcg gtcgcgcaga
    45121 agatcgcgtt catcgcggcc agcgccttcg ggctcgaggc cgtggtcgac gtgtccggcc
    45181 gcctcgccga cgacaagaag aacgacatcc ggatcgaggc cgccatcgcg aagctctacg
    45241 gctccgagat gggctggaag atcgtcgacg agctcatgca ggtgcgcggc gggcgcggct
    45301 acgagaccgc cgagtcgctg ctcgcccgcg gcgagaagcc gatcccggtg gagcaggtca
    45361 tgcgcgacat gcggatcaac cgcatcttcg agggctcgac cgagatcatg cacctgctga
    45421 tcgcgcgcga ggccgtggac aagcacctcg aggtggccgg cgagatcctc gagggcgacg
    45481 gcggcctggg cgagaaggcg aaggtcgcgg tcgaggccgc gaagttctac tcgcgctggc
    45541 tgccacagct cgcggccggc aagggccaga acccgcgcgc gttcgacgag ttcggatcgc
    45601 tggcgaagca cctgcgcttc gtcgagcggg gttcgcgcaa gctggcgcgg tcgaccttct
    45661 atgcgatgac ccgctaccag gcgggcctcg agaagaagca ggccgtgctc ggccggatcg
    45721 tcgacatcgg cgcggagctg ttcgccatct ccgccgcggt ggtgtacgcg acgacgatcg
    45781 gcgcgcagcg cccggagcgc cgcgaggagg cgatcgagct cgcgcagct
//
LOCUS       DASVPL010000102         7123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_248500, whole genome shotgun sequence.
ACCESSION   DASVPL010000102 DASVPL010000000
VERSION     DASVPL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248500"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VF517_05005"
     CDS             <1..1135
                     /locus_tag="VF517_05005"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX8052329.1"
                     /translation="VVQEALAQLRDSAPTVPFDQMRKVIETDLEQPIGEAFAELDPEA
                     VAAASIGQVYRARLHDGRDVAVKVQYPGAESAVRADMQNLGLLLRAARSIAPGLDSKA
                     VAGEIRERILDECDYEAEAIAHRSFAREWRGHPFVHVPDVVTSLSRRRVIVTEWVDGR
                     SFDEVKALGSDERDRFAEIVYRFFVGSLYRLGHFSADPHPGNYLLMDDGRVAFIDFGM
                     NKKISRELIEREREWLKAVMERDPARLRTLLGELGYFDPADEKVTGEWLLEHAWALGG
                     WWLEDRGEFMVTRELVTEVMVAGADPRSDYWELMRHETVPPDFVLAQRMVGLVFAVMA
                     QLDATANWHRIAREFIYGDPPATPLGEAEESFFAKRAPARPAA"
     gene            1132..1797
                     /locus_tag="VF517_05010"
     CDS             1132..1797
                     /locus_tag="VF517_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX8052330.1"
                     /translation="MSPRPGAGTAAAVALDVEKGTRHLRRKDPVMREIVKQVGVLDLE
                     ARRRGRPADAYGALVRSIVGQQLSTKAAATIYGRMIELWGGRTPTPQELLDTDPEAIR
                     AAGMSRPKVAYLRDLAEHVVSGDLELDRLHELTDDEITAELTAVKGLGVWTAHMFLIF
                     HLQRPDVLPVGDLGVRNAVRRAYELDELPDALELTELGEPWRPHRSLASLYLWRSLDN
                     EPG"
     gene            1767..2153
                     /locus_tag="VF517_05015"
     CDS             1767..2153
                     /locus_tag="VF517_05015"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX8052331.1"
                     /translation="MAVAGQRAGLTLGGRSPAQLFCLIGGALLLLRGVIGFVALDTSF
                     DTPGEGWHHLIHLVSGIGLVALAGRSELARAGAIGFGVFYAGIAVIGIVDGEDVLGLI
                     AADPADKTFHALLGAASLAAGLISRR"
     gene            2150..2458
                     /locus_tag="VF517_05020"
     CDS             2150..2458
                     /locus_tag="VF517_05020"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX8052332.1"
                     /translation="MSDRDRLLELVDGATAKRMFGGEGIYAEGVMFAIAMHERLYLKT
                     DDETRPDFEQLGSAPFTMNQQRKSKTFYELPDEVAADDAELAAWAHRAVRAANEAKGR
                     "
     gene            complement(2455..3528)
                     /locus_tag="VF517_05025"
     CDS             complement(2455..3528)
                     /locus_tag="VF517_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052333.1"
                     /translation="MLMPFNEQLDPFFNKILKPVLTEVGYRVTRGDSVLDQQNVLRDI
                     VCGIGSADLIVADLTGLNANVFYELGLAHGLAIPTVLLTQDVATVPFDLRGYRLKPYD
                     TQFHKVAEFKDWLRDVATEHAAGKIRFGNPVTDFLPADIRVERGRGLVGDEADFALRD
                     LTPALPRVIDCVESMLEDVDFLTDRAASAAVLTGFHLPRFRRVGNQYLNKRGPDGKVV
                     ADGIVESLRAFAEELEPVLVRLEQRNKELRTGPLASMTFSNLGSLREVDVIKRTMLRL
                     NSAMPELKDRTERLMESLVPLSTKRRDVQSEVQRNQAIWERVVNTADSASAFGQRMLH
                     VLDVATEMADNEEAVSPVEGGAI"
     gene            complement(3594..3947)
                     /gene="erpA"
                     /locus_tag="VF517_05030"
     CDS             complement(3594..3947)
                     /gene="erpA"
                     /locus_tag="VF517_05030"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX8052334.1"
                     /translation="MVELTDVAAGKIQTLLSDEEHANAGLRVAVRGGGCSGFQYQLAL
                     DEKRDGDKIFEHDGIRIIVDEQSLQYVDGSTVDYTESLMGAGFQVNNPNVVAACGCGS
                     SFRVADEEPSCGAEV"
     gene            3991..4230
                     /locus_tag="VF517_05035"
     CDS             3991..4230
                     /locus_tag="VF517_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX8052335.1"
                     /translation="MPNAEDLKRRIEDALPGSTAAVEDLTGGGDHFRAEIVSDRFAGL
                     SRIEQHKLVYSVFGDEVGGPIHALSIKTSTPGGTA"
     gene            4227..4622
                     /gene="grxD"
                     /locus_tag="VF517_05040"
     CDS             4227..4622
                     /gene="grxD"
                     /locus_tag="VF517_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX8052336.1"
                     /translation="MTNEEIRDFIQNAIDENQVMLFMKGTPEQPACGFSMRTSAALNA
                     LGVEYSALDILPDPRIRQELSAISSWPTIPQLFVDGELIGGCDIVTEMYESGELAARL
                     GVPQPDAEPQPPAAAAPATAPLGIENRLS"
     gene            complement(4626..4841)
                     /locus_tag="VF517_05045"
     CDS             complement(4626..4841)
                     /locus_tag="VF517_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052337.1"
                     /translation="MAAPVAIYRAVRTARRVAPLAAEAYRRWNQLSPAEKERHKERVR
                     LYAQRGQDIGREALRRAEEQRRKRGGR"
     gene            complement(4841..5110)
                     /locus_tag="VF517_05050"
     CDS             complement(4841..5110)
                     /locus_tag="VF517_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8052338.1"
                     /translation="MAILYRCKTPTNRLCACGKVARRLDRAGIAYEEIRVPVRRRDRD
                     ELEELTGQTWAPVLIIGDEVIHESHRICEHIEWRSERGPEPAAAV"
     gene            complement(5121..6206)
                     /locus_tag="VF517_05055"
     CDS             complement(5121..6206)
                     /locus_tag="VF517_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8052339.1"
                     /translation="MPRTLVTGGNGFVGSPIVRLLVERGDDVRLTVRRRSRLENVADL
                     DLDQVECDVRDRRAVRRAMKGVDRVFHVAGLVSQRPEDADALFDTNVVGTRNVLEEAL
                     RADVGRVVHTSSVVAIGPAPWGETADERQLFTAGHLGIPYVNSKHEAEAEAMRLAARG
                     LPVVVVNPGSVFGPGDVYGRATATVRRFMLGRVPAYVSGGLNVVDVDDVARGHLLADT
                     IGKVGERYILGNRNYTLDRLFADLARLSGIEAPAVKLSAPLARRLVQVAEAAGVRTGV
                     TLTDVKISSQWWCYRNGKAKRELGWTTTPHEDTIEATVGWYRDREGDRLARNPRTQPV
                     PLKVAAAALGAFEGAAGVARRMWPLTT"
     gene            complement(6219..6608)
                     /locus_tag="VF517_05060"
     CDS             complement(6219..6608)
                     /locus_tag="VF517_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX8052340.1"
                     /translation="MPEIDRDQVSQLLDQREREIEERRQSVAPDSAGRDAELADYDNH
                     PGDQGTETFMQEMDETTDMILDEEATRVREARQALENGTYGTCVDCGKEIPAARLEAI
                     PESVRCVDDQRAYEARLRQGGGPAATH"
     gene            6662..>7123
                     /locus_tag="VF517_05065"
     CDS             6662..>7123
                     /locus_tag="VF517_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX8052341.1"
                     /translation="MPPVAVVTDTTGYLPVEVVEANGIELVSLYVNFGGERTEREADL
                     RDYDQFYDELRSAETLPTTSQPSIGDFIGVYEPLLAEGRDVVSVHISSGISGTSGAAQ
                     QAKEALERDGRGGERVHVLDSATAAGGLGLVALAAARRAASGADAEAVLAHA"
BASE COUNT         1008 a         2534 c         2502 g         1079 t
ORIGIN      
        1 cgtggtccag gaagcgctcg cgcagctccg cgactccgcg cccacggtgc cgttcgacca
       61 gatgcgcaag gtgatcgaga ccgatctcga gcagccgatc ggcgaggcgt tcgccgagct
      121 cgaccccgag gccgtggcgg ccgcctcgat cggccaggtc taccgcgcgc ggctgcacga
      181 cggccgcgac gtggcggtaa aggtgcagta cccgggcgcg gagtccgcgg tgcgcgccga
      241 catgcagaac ctcgggctgc tgctgcgcgc cgcccggtcg atcgcgccgg ggctcgacag
      301 caaggcggtc gccggcgaga tccgcgagcg gatcctcgac gagtgcgact acgaggcgga
      361 ggcgatcgcc caccgctcgt tcgcgcgcga gtggcgcggc cacccgttcg tgcacgtgcc
      421 cgacgtggtc acgtcgctct cgcgccggcg cgtgatcgtg accgagtggg tcgacggccg
      481 ctcgttcgac gaggtcaagg cgctcggcag cgacgagcgc gaccgcttcg ccgagatcgt
      541 ctaccgcttc ttcgtcggct cgctctaccg gctggggcac ttctccgcgg acccgcatcc
      601 cggcaactac ctgctgatgg acgacggccg cgtggccttc atcgacttcg gaatgaacaa
      661 gaagatctcg cgcgagctga tcgagcgcga gcgcgagtgg ctcaaggccg tgatggagcg
      721 cgaccccgcg cggctgcgga cgctgctcgg cgagctcggc tacttcgacc cggcggacga
      781 gaaggtcacc ggcgagtggc tgctcgagca cgcgtgggcg ctcggcggct ggtggctcga
      841 ggaccgcggg gagttcatgg tcacgcgcga gctcgtgacc gaggtgatgg tcgcgggggc
      901 cgacccccgc tcggactact gggagctgat gcgacacgag accgtgccgc ccgacttcgt
      961 gctggcgcag cggatggtgg gcctcgtgtt cgccgtgatg gcgcagctcg acgcgacggc
     1021 caactggcat cgcatcgcgc gcgagttcat ctacggcgac ccgccggcca cgccgctggg
     1081 cgaggccgag gagtcgttct tcgcgaagcg cgcgcccgcg cggccggccg cgtgagcccg
     1141 cgcccgggag ccggcaccgc cgcggccgtg gcgctcgacg tcgagaaggg cacgcgccac
     1201 ctgcgccgca aggacccggt tatgcgggag atcgtcaagc aggtgggggt gctcgacctc
     1261 gaggcgcgcc gccgcgggcg gccggccgac gcctacggcg cgctcgtgcg ctcgatcgtc
     1321 ggacagcagc tctcgaccaa ggccgccgcc acgatctacg ggcgcatgat cgagctgtgg
     1381 ggcgggcgga cgccgacacc gcaggagctg ctcgacaccg accccgaggc gatccgcgcg
     1441 gccgggatgt cgcgcccgaa ggtcgcctac ctgcgcgacc tcgccgagca cgtcgtgagc
     1501 ggggacctgg agctcgaccg cctgcacgag ctcaccgacg acgagatcac cgccgagctg
     1561 accgcggtga agggactcgg cgtgtggacg gcgcacatgt tcctgatctt ccacctacag
     1621 cgtccggacg tgctgccggt tggcgacctc ggggtgcgca acgccgtcag gcgcgcgtac
     1681 gagctcgacg agctgcccga cgcgctcgag ctgaccgagc tgggtgagcc gtggcggccc
     1741 caccgctcgt tagcctccct gtacctgtgg cggtcgctgg acaacgagcc gggctgaccc
     1801 tcggcgggcg ctcgcccgcg cagctcttct gtctgatcgg gggtgcgctg ctgctcctgc
     1861 gcggcgtgat cgggttcgtc gccctcgaca cgagcttcga cacgcccggc gagggatggc
     1921 atcacctgat ccacctggtg tcgggcatcg gcctggtggc gctcgccggc cgctccgagc
     1981 tggcgcgcgc gggcgcgatc ggcttcggcg tcttctacgc gggcatcgcg gtgatcggga
     2041 tcgtcgacgg cgaggacgtc ctcgggctta tcgcggccga ccccgccgac aagaccttcc
     2101 acgcgctgct cggggcggcg agcctcgccg ccgggctgat ctcgcggcgg tgagcgaccg
     2161 cgaccgactc ctcgagctgg tcgacggcgc caccgccaag cgcatgttcg gcggcgaggg
     2221 catctacgcc gagggcgtga tgttcgcaat cgcgatgcac gagcgcctct atctgaagac
     2281 cgacgacgag acccggccgg acttcgagca gctcggctcc gccccgttca cgatgaacca
     2341 gcagcggaag agcaagacct tctacgagct accggacgag gtcgccgcgg acgacgccga
     2401 gctggcggcc tgggcgcacc gcgcggtgag ggcggccaac gaggcgaagg gacgctagat
     2461 cgccccgccc tccacggggc tgaccgcctc ctcgttatcc gccatctccg tcgcgacgtc
     2521 caagacgtgg agcatccgct ggccgaaggc ggacgcgctg tccgccgtgt tgacgactcg
     2581 ctcccagatc gcctggttgc gctgaacctc cgactgaacg tcgcgccgct tcgtgctgag
     2641 ggggaccagc gattccatca gccgctcggt ccggtccttc agctcaggca ttgctgagtt
     2701 gagacgcagc atcgtccgct tgatcacgtc cacctctctg agggacccga ggttcgagaa
     2761 cgtcatgctc gccagcggac cggtgcgaag ctctttgttg cgctgctcga gccggaccag
     2821 gacgggctcg agctcttcgg caaatgccct gaggctctcg acgatcccat ccgccacgac
     2881 cttcccgtcc ggacccctct tgttgaggta ctgattgccc acgcgacgga agcgcggcag
     2941 gtgaaagccc gtgagcaccg cggcgctcgc cgcacgatcg gtcaggaagt ccacgtcctc
     3001 gagcatgctc tcgacgcagt cgatcacacg cggtagggcc ggggtaaggt cgcgcagcgc
     3061 gaaatcggcc tcgtcgccga cgagcccacg tccgcgctcg accctgatgt ccgcaggcag
     3121 gaaatcggtg accgggtttc cgaagcggat ctttccggcc gcatgctcgg tggcgacatc
     3181 ccgtagccaa tccttgaact cggccacctt gtggaactgg gtgtcgtacg gcttcaggcg
     3241 atagccccgc agatcgaacg gcaccgtcgc gacgtcctgc gtaagcagga ctgtcgggat
     3301 cgcgagcccg tgcgcgagac ccagctcgta gaagacgttc gcgttgagcc cggtcaggtc
     3361 ggcgacgatc aagtcggccg acccgatgcc gcagacgatg tccctgagga cgttctgctg
     3421 atcgagaacg ctgtcgccgc gcgtgaccct gtagccgacc tccgtgagga cgggcttcag
     3481 gattttgttg aagaagggat ccagctgctc attgaacggc atgagcacga agaccttcgg
     3541 accctcatcg tgaccatcca ctcaagatga gtgtacggcg ggcggtgctg gcgctagacc
     3601 tcagcgccgc acgacggctc ttcgtccgcg acgcggaacg aggacccgca cccgcacgcc
     3661 gctacgacgt tcgggttgtt cacctggaag ccggctccca tgagcgactc cgtgtagtcg
     3721 accgtcgagc cgtccacgta ctggaggctc tgctcgtcga cgatgatccg gatgccgtcg
     3781 tgctcgaaga tcttgtcgcc gtctcgcttc tcgtcgagcg cgagctggta ctggaagccg
     3841 gagcagccgc ccccgcgcac ggccacgcgg agacccgcgt tcgcgtgctc ctcgtcggag
     3901 agaagcgtct ggatcttgcc tgcggctacg tcggtgagct cgaccacgtc ctcgattgta
     3961 gtcgccaggt ccagcgctat gctcgaattc gtgccgaatg cagaggacct gaagcggcgg
     4021 atcgaggatg ccctgccggg ctcgaccgcc gctgtcgagg acctcaccgg gggcggcgac
     4081 cacttccgcg ccgagatcgt gtcggatcgg ttcgcggggc tctcgcggat cgagcagcac
     4141 aagctcgtct acagcgtgtt cggcgacgag gtcggcggcc cgatccacgc gctttcgatc
     4201 aagacatcca cgccgggagg caccgcatga cgaacgaaga gatccgcgac ttcatccaga
     4261 acgcgatcga cgagaaccag gtgatgctct tcatgaaggg cacgcccgag cagcccgcct
     4321 gcgggttctc gatgcgcacg tccgcggcgc tcaatgcgct cggcgtcgag tactcggcgc
     4381 tcgacatcct gcccgacccg cgcatccgcc aggagctctc ggcgatctcg agctggccca
     4441 cgatccccca gctcttcgtg gacggcgagc tgatcggcgg ctgcgacatc gtcaccgaga
     4501 tgtacgagtc cggcgagctc gccgcgcgcc tcggcgtgcc gcagcccgac gccgagccgc
     4561 agccgcccgc ggcggcggcg ccggccacgg cgccgctcgg catcgagaac cgcctgagct
     4621 agggactagc ggccgccgcg cttgcggcgc tgctcctcgg cgcgccgcag cgcctcgcgg
     4681 cctatgtcct gcccgcgctg cgcgtacagc cgcacgcgtt ccttgtggcg ctccttctcg
     4741 gccgggctga gctggttcca gcggcggtag gcctcggcgg cgagcggcgc cacgcggcgc
     4801 gcggtgcgca cggccctgta gatggcgacg ggagcggcca tcagacggcc gccgccggct
     4861 cggggccgcg ctcggagcgc cactcgatgt gctcacagat gcggtgcgac tcgtggatca
     4921 cttcgtctcc gatgatcagt acgggcgccc acgtctgtcc ggtcagctcc tcgagctcgt
     4981 cgcggtcgcg acgccggacg ggcaccctta tctcctcgta cgcgatacct gcccggtcga
     5041 ggcgccgcgc aaccttgccg cacgcgcaca gccggttcgt gggggtcttg cacctgtaga
     5101 ggatcgccat gcgactcagg ttaggtggtg agcggccaca tgcggcgagc cacgccggcg
     5161 gcgccctcga acgcgccaag cgccgcggcc gcgaccttca gcggcaccgg ctgggtgcgc
     5221 gggttgcgtg ccaggcgatc gccctcgcgg tcgcggtacc agcccaccgt ggcctcgatc
     5281 gtgtcctcgt gcggagtggt cgtccagccg agctcgcgct tggccttgcc gttgcggtag
     5341 caccaccact ggctcgagat cttcacgtcg gtgagcgtga ccccggtccg cactccggcc
     5401 gcctcggcga cctgcacgag ccgccgcgcc agcggcgccg agagcttcac cgcgggcgcc
     5461 tcgatcccgg acaggcgcgc gaggtcggcg aagaggcggt cgagcgtgta gttgcgattc
     5521 ccgaggatgt agcgctcccc cacctttccg atcgtgtccg cgagcaggtg cccgcgcgcg
     5581 acgtcgtcca cgtccaccac gttcaggccg cccgacacgt acgccggcac gcggccgagc
     5641 atgaaccgcc gcacagtcgc ggtcgcccgg ccgtagacgt cgccggggcc gaacaccgag
     5701 cccgggttga ccaccaccac gggcagcccg cgcgcggcca ggcgcatcgc ctcggcctcg
     5761 gcctcgtgct tcgagttcac gtacgggatg cccaggtgcc cggcggtgaa gagctgccgc
     5821 tcgtcggcgg tctcgcccca cggcgccgga ccgatcgcga ccacgctcga cgtgtgcacc
     5881 acccgcccga cgtccgcgcg cagcgcctcc tcgagcacgt tgcgcgtgcc gacgacgttg
     5941 gtgtcgaaca gcgcgtccgc gtcctccggc cgctgcgaga cgagcccggc cacgtggaag
     6001 acgcggtcga ctcccttcat ggcgcgcctg accgcgcggc ggtcgcgcac gtcgcactcg
     6061 acctggtcga ggtctaggtc ggcgacgttc tcgagccgcg agcggcggcg gaccgtgagc
     6121 cgcacgtcgt cgccgcgctc gacgagcagg cggacgatcg gggagccgac gaagccgttc
     6181 cctccggtga cgagggtcct gggcaaggcc ggcggcggct agtgcgtggc ggcgggcccg
     6241 ccgccctggc ggagccgggc ctcgtaggcg cgctggtcgt cgacgcagcg aacgctctcg
     6301 gggatcgcct cgagccgcgc ggcggggatc tccttgccgc agtcgacgca ggtgccgtag
     6361 gtgccgttct cgagcgcctg gcgcgcctcg cggacccggg tggcctcctc gtcgaggatc
     6421 atgtccgtgg tctcgtccat ctcctgcatg aacgtctcgg tgccctggtc gccggggtgg
     6481 ttgtcgtagt cggccagctc ggcgtcgcga cccgcggagt ccggggcgac cgactggcgg
     6541 cgctcctcga tctcgcgctc gcgctggtcg aggagctggc tgacctggtc tcgatcgatc
     6601 tcgggcatgg cggcggccca gtctacgagg tggtgtccgc gacgtgtacg ctccgccgcc
     6661 gatgccccct gtcgccgtcg taaccgacac caccggctat ctcccggtgg aggtcgtgga
     6721 ggcgaacggg atcgagctgg tctcgctcta cgtgaacttc gggggcgagc ggaccgagcg
     6781 cgaggcggac ctccgcgact acgaccagtt ctacgacgag ctccgctcgg cggagacgct
     6841 gcccaccacc tcgcagccgt cgatcggcga cttcatcggg gtctacgagc cgctgctcgc
     6901 cgagggccgc gacgtcgtct cggtccacat ctcctccggc atctccggca cgagcggcgc
     6961 ggcgcagcag gccaaggagg cgcttgagcg cgacggccgc ggcggcgagc gcgtgcacgt
     7021 gctcgactcc gccaccgcgg cgggcgggct cggcctggtc gccctggccg cggcgcggcg
     7081 ggcggcgtcg ggggccgatg ccgaggcggt gctcgcccac gcc
//
LOCUS       DASVPL010000103        22878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_13102, whole genome shotgun sequence.
ACCESSION   DASVPL010000103 DASVPL010000000
VERSION     DASVPL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22878
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13102"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2251
                     /locus_tag="VF517_05070"
     CDS             <1..2251
                     /locus_tag="VF517_05070"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEX8052342.1"
                     /translation="GLGGRTIKLDPGTPGDPSDDISTTTAGDGSYSFPGLKPGTTYRV
                     YDEGESGWTCSEPGSPCEYSVPTQSGDGTVERDFGAWRPASISGATFEDRDADAARDA
                     GEPALTGRTVYIDADGSGGLTSGDPTTTSGAGGSWSFTGLKPGAYTVREELPSGWTCS
                     VPAGACAHVETLVSGDAPTGRDFGSWRGVTIGGVKYEDRDADGDRDTGEPGLGGRTIK
                     LDPGTPADPSDDLSTTTAGDGSYSFPGLKPGTTYRVYDEGESGWSCSEPGSPCEYSVP
                     TQSGDGTVERDFGAWRPASLSGVSFEDGDRDGTRDAGEPGRGGWTVTLDPGTPADDSD
                     DVDTTTAADGGYSFTGLRPDMAYTLTIESRSGWTCTFPAGSCDHARTPASGDAVTGLD
                     FGAYAEASLEVRQELDPVGDPGRFDLTVGTVLAKAGAGHGDSTGRRSVAPGDHAVTSK
                     AASGTAGGDYDSTLECRASGGSGSVVPAPGGTVTLAAGDDVLCTFTSVRRPAPPADPP
                     ADPPADPPADPPVPPADPPADPPKPPLDPPAPPAPPADECGANGADPVAPGCEQPPAP
                     ETGEACLKRPVLTWVRGSGIRRVVFYLDGKRRATVMRPDGEGRYALRTDRRTLTAGKH
                     EVRAQVFFRRAGRAPKTLRFTIEPCLKLETSSAIETTRPGELDVCAGGAFRAFVRGDT
                     IRRVVFYLDGKKVKSTKVADWRGHYWVDVRPSALSVGGHELRARMYFISGSRQRSREL
                     KLGFRRCGG"
     gene            2285..2704
                     /locus_tag="VF517_05075"
     CDS             2285..2704
                     /locus_tag="VF517_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052343.1"
                     /translation="MELPMTARLRLIVLLGLVALLTPAAAARATEKPLLPPEVLLAQE
                     GTPAPPATDQEDEELGEEIPDEDGSDPKPPPEDDDSGSEGTGSSAPPADPPAPETRAA
                     TPVRSLPRTGQDPIPLLVTGMFLLGTGLVLRAAVPRT"
     gene            2718..3671
                     /locus_tag="VF517_05080"
     CDS             2718..3671
                     /locus_tag="VF517_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8052344.1"
                     /translation="MRAVQINDFGGPEVLELVDLDVPEPGEGQVLVKVSRAGVNFADT
                     HQRENAYVKKFGLPLVPGNEVVGTTEDGTRVVAMTGGAGGYAEYALAPADSAFPVPDG
                     VSDETALALLIQGLTAWHLHRTCARIAEGESVVVHSAVGGVGSLATQLAKPMGAGRVI
                     ATAGSEERRAKALELGADAAVDPDEEDLTAALIEANGGEPLDVVLETSGGRVFDASFE
                     ALAPFGRLVVYGISSREQNEVRTGKLLRGSRSVIGFWLMHCLERPELLREPLADLFDR
                     AARGELRALAGGTYPLSEVRRAHEDLVGRRTTGKLVLDPTA"
     gene            3681..5390
                     /locus_tag="VF517_05085"
     CDS             3681..5390
                     /locus_tag="VF517_05085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8052345.1"
                     /translation="MTKFADLRLSEPLLQALAHLGYDQPTPIQEQAIPELLDGNDVIG
                     QAQTGTGKTAAFGLPLLEYVDKDMPEVQALVLTPTRELCIQVTQALRAYGERKGIEVV
                     AVFGGAPIRDQAAFLRQGAQVVVGTVGRVMDMINRHYLFLTEARYVVLDEADEMLDLG
                     FLEDVETILGRCPSGRQTALFSATMPPAIKKLAEKRMFDPITIKVRAAQLTIDTVEQF
                     YVEASDREKPDALARVLKAERPDQAIIFVRTKIGADRLARRLGDDGIRVKALHGDMSQ
                     GQRDGVMIAFKGGRERLLVATDVAARGLDIKGVSHVVNYDIPNSPDIYVHRIGRTGRV
                     GRSGRAITLITPKQRSDLDAVERHANTEIEEWSPGVVASAEAAEHRADSAAQATSSGD
                     SEAATSVAREAKAPGRDAAPKPGAREAAPAAAEPEPAAGEAASSAADAAPAAREAAPA
                     ESAPATNGDGAEQRPRRRRSRGDRETRRPRHTKPRKDPDGTPRSKLLVSAGRAHGLEP
                     ADLIRAIVENARLDGEDVTGVRVLERFSFAEVPTEHADEVVRSVGGRSVKGTELRMEV
                     TRT"
     gene            5387..5848
                     /locus_tag="VF517_05090"
     CDS             5387..5848
                     /locus_tag="VF517_05090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8052346.1"
                     /translation="MSQATLHTNHGPIEVEFFDEDAPKTVENFRKLAGDGFYDGLVFH
                     RVIKDFMIQGGCPQGRGTGGPGYTFEDEFNQHKVERGALAMANSGPNTNGSQFFIVTT
                     EAAPWLDGKHTVFGRVTSGMEAVDAIENTPTGAGDRPREDAVIERVELPEA"
     gene            5921..7474
                     /locus_tag="VF517_05095"
     CDS             5921..7474
                     /locus_tag="VF517_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8052347.1"
                     /translation="MAVESQVSEATQNGHGNGKTFAVENPATGATIAHLPDMDAAQVK
                     ALVDRARAAQPAWEALGFEGRARYMFQFRKWLVDNRDRVARTVMDETGKTREDAFLAD
                     VFTTADALGFWAKKAPEYLKDERVRSTNPFVLGKKVVVRYRPFGVAGVIGPWNYPIAN
                     CFGDAVPALMAGCTVVMKPSEVTPLASLLVEEGLREIGLPEDVCLVATGTGAAGAALV
                     DEADVVMFTGSTATGKKVAARAVESLTPFSLEMGGKDPMIVLRDADVERAVNEAVYWG
                     MANGGQICQSVERVYVEEPVYDEFVEKVVERVKALKQGPSDGPGSAEIGAITFAPQQE
                     IIERHMRDAVDKGAEIRVGGKAAEKGAGRWWEPTVLTNVDHTMECMTEETFGPTLPIM
                     KVRDEHEALRLANDSPYGLNSSVYTRDIEKGERIARGLTAGNACVNDTVVNYAAADAP
                     FGGTGESGMGVRHGPQGIRKYCGVQTILTSRFHMKKEAYMFPYSPMKSKFVERLSVTL
                     YGRIPRRYR"
     gene            7475..7912
                     /locus_tag="VF517_05100"
     CDS             7475..7912
                     /locus_tag="VF517_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX8052348.1"
                     /translation="MATRRAEATWEGSLAEGSGTTELDSGVAPALDVNWRARTEGGAQ
                     TSPEELLAASHAACFAMALSHELSEAGNQPQRLTVGAAVSFGPVEGGFAVTKSELKVR
                     GVVPGIDADAFEKAAQGAGEGCPISAALKGNVEISVDAQLESA"
     gene            8052..9050
                     /locus_tag="VF517_05105"
     CDS             8052..9050
                     /locus_tag="VF517_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8052349.1"
                     /translation="MGAIALGFASAVVWGAADFLGGLKSRQVALFAVMLLSQAAGLAP
                     LLIAAAVRGEPPPGADILPWCVLGAVAGTVGLAAFYRGLAVGQMGVVAPISASAAAIP
                     VVFGVASGERPGAVQVAGIAVAIVGVALASWEPREEEPPAQRAAAEAGEAEHPEAGGA
                     EHPEAGRAWHAETGAQHPDAGGAQHAVPRRGVGLANASTGAGLALVAAVGFGLFFVGL
                     DEASDRDLLWALVLTRSVSTGALVLAALATRPNLAMTLADGRTIAAVGILDVSANALF
                     AAGTTVGLVSVVSVLGSLYPVTTIVLARFLLGERLGPVQRGGAVTALVGVALISAG"
     gene            complement(9069..9431)
                     /locus_tag="VF517_05110"
     CDS             complement(9069..9431)
                     /locus_tag="VF517_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052350.1"
                     /translation="MIRSLQLALGVGRIAFGIGLMTVPRPMAGLLMGTAAEQTGARFA
                     ARSYGTRDLILGGGLVYAALGGSDVRPWLAAGIAADAMDAGLQLLEWDELVPGRRAAG
                     VTAASTVTAAGIALLSRS"
     gene            complement(9428..10039)
                     /locus_tag="VF517_05115"
     CDS             complement(9428..10039)
                     /locus_tag="VF517_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX8052351.1"
                     /translation="MSRGLLVDFGGVLTTNVFESFRAFCEAEGLEPNTVKEMFRSRGE
                     GLDLLRQLERGELTVEQFEPKFGAVLGIERTEGMVERLFAGVGPDERMVDAVRRARAA
                     GVPTGLISNSWGRTTYDEQLIDELFDAAVISGDVGLHKPEPAIFHLGADKIGVPPADC
                     VFVDDLRENCEGAAAVGMTPILHRGADRTVPELERLLGLELAA"
     gene            complement(10036..11115)
                     /locus_tag="VF517_05120"
     CDS             complement(10036..11115)
                     /locus_tag="VF517_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX8052352.1"
                     /translation="MDVVRTPDEARAQERPPLIVLDPLTEYLDSRGLGSGPVEAEPIG
                     QGHSNVTYLIRRGDERFVLRRPPRPPLPPSAHDVLREARLLDAIESAEVRTPTVLAAC
                     DDESVLGVPFYVMEYLEGAVIVSEVPEELSSPGERRRICLELVDALAEMHAVDWRACG
                     LEGFGKPTGYLDRQLRRFNGLWDINKTRELPAVQEVGEWLAANKPESPAATIVHGDYR
                     LGNTMFASDAPARLIAIFDWEMATIGDPLADVGYLTATWSQPAGGDREPTMFDMLTVT
                     HEEGFLSRDELVARYEEASGRSMSDLRWYQALALWKAAVFMEGNYKRSLAGATDDPYL
                     KLFDVGVPQLAQAALGITQMSAPST"
     gene            11166..11843
                     /locus_tag="VF517_05125"
     CDS             11166..11843
                     /locus_tag="VF517_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8052353.1"
                     /translation="MRRLAIALPLALLLCSPAMAADQSVSATNSNTFTPSTVTVNPND
                     TVTWTNQGGFHNVVFDDGSFTDPAQPSFSMWTVQRKFPTAGSFKYHCGYHGTSMSGTV
                     VVQAAQQPPAGGQPDTTPPDIDDLKLVPATFCNKKTSKCKRVGTLIRFTLDEDAKISG
                     RVVNRKTGKKAGSVTISASAGANEFDYSGKGLALGRYRLELTPRDAAGNKAAKPTRAN
                     FTVATKR"
     gene            complement(11869..12477)
                     /locus_tag="VF517_05130"
     CDS             complement(11869..12477)
                     /locus_tag="VF517_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX8052354.1"
                     /translation="MTDGAWIAVEGLIGAGKTTTANLLAERLGAQPLLERVAAHPLLP
                     RFYEEPDRFALETELVFMALRLHEAKAVEPGESVVSDFTPAKTSVFASVNVSDKDACL
                     IEVFEAHVWEDLARPGIAVFLDVPPKVCLARIRARGREFEQGLAITYLEALRAAYFVR
                     LDALAASVITLELDGSSSAEEVTDAVAASIAGVQADERRHEG"
     gene            complement(12470..13657)
                     /locus_tag="VF517_05135"
     CDS             complement(12470..13657)
                     /locus_tag="VF517_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052355.1"
                     /translation="MSNIDAVMATISERGDWRRIRGLYKQFSAIAPDVEPPTDLAGGL
                     AEALTRTFASYEDHETSFVTDLAQLMGAIDGYSDSPPPERFWFLYKDIRLENFPAYRA
                     HTGGKRRPRPFEAAVETVRRIADLNQVRDRLERLSTSAILGGSASYGRFYNTAGSVRE
                     RPSDLDLLVVIPSYDDLPHIVTAMSELAFIDPKGIDVFHDRVAYFSEVRGEHGRCAFQ
                     QKLSMWPAPSHAGPAYHGLPGFYSLALHAFDLDDFNYLALRDIHALHDGFSRTLSEYR
                     GDKPAAKDELRSFAGTRHSVDTAYVEVRNGVVAKTRHCEVTDERFYPGVHMNLLLPQL
                     EVRWESPRARVRLALLDLRWKLLARLAEERRLRSHEIQRLSLSHTRVAAFAPHVTRRV
                     DRD"
     gene            complement(13654..16455)
                     /locus_tag="VF517_05140"
     CDS             complement(13654..16455)
                     /locus_tag="VF517_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8052356.1"
                     /translation="MTTDAQREQARRDDAVAVLRAVWPDAPAELDEFLGKNFTFKVGN
                     RNRALRSPTGDSLCGHRLELPLRNVQIMVGGGGVVIPCREADVPVDYALKVVRPTLYE
                     EARERGEISEEYEKAEDERRTHLPLSHENIAKLYGTDTVIVKRSQVHDLKLGATLIEW
                     VQGAQPFGRYLEEGAADAWQVADLIQQASRALVQLHESGKVHWDVKGDNVLVSAQGVV
                     KLMDLGNARPLRPPGDKYETRYEAETTLLNLPPELERRALKIRGNLTAAGKRVSNNRA
                     PCHFKAGEGAWDRPWLDLYMLAREVNRVFGFDEASAALDDPADGVDAVALCAARRDSI
                     FGSEAGGFVCAYLRRLVGRLLAADSPDATPFYEDADAVVRALERLLPEYGEATDVPEL
                     EAVSQHVLRVPPFNNVPWTPRVNALMNSRPLLRLKRHRQLATVRDVFPGAEHTRWEHV
                     SGALVELLQRIRALFADRSTVSIRLDTSKLDVTALILATLVHDLAHPAYGHQLEESPL
                     IPPNEHHESYGRAVLRATIGDRRKTDAIGKDAEALVEVLDQYWVSSDMSRQQLVGRAI
                     EILEAGAGTPGADEDPTAQSQRLHLETLRSLIDGQLDADKADYLVRDAHHCGVEYASG
                     IDRRRLDQALTTLEVPIDGVLRGTIGVTQKGVLPLESMLIARYQMFRAVYWQHTVRAM
                     TVMLQEAVEEHVVSSDDSKFVASRLRDLLHQFRELSDDAALDWLEAKLARRQKHVREL
                     CEGVRGNRRNVFWHVAEFQPRTELLDELADAKPVDDKVYDALQQHWTRGARADGVAVL
                     LSRRRLRSNVAAAITAEANESPQIKSQVKLDTHDVLLDIPLAGRDEVRDLYVLVERGT
                     EKRTAPVQDLTPLAHAVTQAFQRSARPARIFLSPSVCRPLLEVGEWERFQDVASDAVR
                     KELTDQLPLFDIADGAGRR"
     gene            complement(16993..17433)
                     /locus_tag="VF517_05145"
     CDS             complement(16993..17433)
                     /locus_tag="VF517_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX8052357.1"
                     /translation="MRIAVSSDERTGVAEAVVEELRRRGHEPVVHGALAEGERPDWAW
                     ASEAAARDVAGGRTEQGIVCCWTGTGASIAANKVDGVRAALCADAETARGARRWNDAN
                     VLALSLRTTSEALLGEILDAWFEGEPSPDHDDAANIRHVDEIAG"
     gene            17499..18878
                     /locus_tag="VF517_05150"
     CDS             17499..18878
                     /locus_tag="VF517_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8052358.1"
                     /translation="MLTRRWPLAVALAALMLGVLATPSLAAVQTKSFDYPVQMDPFEV
                     QQATAIVNPGIEGYVTGMSVDVVNADGTQVPINRVMLHHIVFARLGVKHPGCDSITQF
                     DSKTKIPAAGEPMYAAGEERQVLALPPGYGYHMTANAPLYMVWMLMNHRGVKDNANIR
                     YTITYEDSEQLKPVRPLWMDVANCQADPVYNVPGDGKPGSVHERTYDLVAPESGRIVA
                     GGGHVHGGGIETAISEPDCNNRTVMASKPAWGMPDHPFYKVRPILHEPGPVSMSGWLS
                     GQGYPVQKGQRIRLTSRYDNSRPHTRVMGINVIYLAPGEVNEPCGPPPTDGVNIQAAE
                     LRGEPYRTKTPKFTVPLTGVDSKGRAVTISRPPGKTRAVKSGTTIKARDSYFSIPNVE
                     VKKGSKLSWQFGTPTLHNVTLANGPRGFASDNLNEGRKFNMKFKVPGTYRLMCTLHPV
                     GMTQTVTVR"
     gene            18906..19757
                     /locus_tag="VF517_05155"
     CDS             18906..19757
                     /locus_tag="VF517_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX8052359.1"
                     /translation="MPELPEVEIAVRRLDAALEGARVESALAPGMVTMKTFDPPLDAL
                     AGRTIERVRRIGKMPVVEFSGDLALLVHLMSAGRLQLFPDRASLRDKRSRLLVRIDDG
                     RELRLREFGTQQRAWAKLLPLADLATDEAVAKLGPEAYPPLPLEEFARLVDQPHHLHP
                     MLRDQRTIAGIGRSWVDEILWQAGLSPFRKGNDLEPEEVERLHAAVGDVLGGALEHYE
                     KVVGESLPDKAPMPLQVHRKPGEPCPRCGATIEAVHFKDYVMCYCPQEQTGGRVLKDR
                     RLSRLLK"
     gene            19754..19960
                     /locus_tag="VF517_05160"
     CDS             19754..19960
                     /locus_tag="VF517_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052360.1"
                     /translation="MRERLLGLPRWRIALDVAICGLFAGLLTLGLGALGDAELDAVAR
                     DALAVGIAAALFYAFAVYRVRRGL"
     gene            19957..20268
                     /locus_tag="VF517_05165"
     CDS             19957..20268
                     /locus_tag="VF517_05165"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX8052361.1"
                     /translation="MRALAGFVPHCLILFRRLLADDRVSRARKLLLVAVVAYLAFPLD
                     LIPDFIPVLGQLDDALVVALALRAIARGAGAELLSEHWPGPDASLRTVLRLAGARPSP
                     T"
     gene            20278..21273
                     /locus_tag="VF517_05170"
     CDS             20278..21273
                     /locus_tag="VF517_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX8052362.1"
                     /translation="MPPVATLGDSFRSFFDAVEEFFSNLAAVHWGSLVVALIAFGVYL
                     TLRSRASYNILRAAYPEEQFRWREIWGAYMAGYGFNSVIPARGGDLVRLFLTKNAIPN
                     ATYPATGAAFTVELGFDLVFGGTILIFAFTQGVFPKPPDLSKLNAFELSYFAQHPRFT
                     LFLLTAMAIGAIVATAMLSARVRAFWTGVRQGLTIAFDRRRYLREVFAIQAAGWVFRF
                     TAFWFLLEAFNVGGSVKNVLLVLGVQAVSAALPFTPGGAGAQQALLVKVFGGSSTVAA
                     YSVGQQIAIAAFTFAVGFAALAFIFRVRSFKEVIARGREQREEEARRKEAAAGPV"
     gene            complement(21270..21551)
                     /locus_tag="VF517_05175"
     CDS             complement(21270..21551)
                     /locus_tag="VF517_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052363.1"
                     /translation="MAEDDPFGGLFGDPEELRRRMQELGEQMQGAQKVAWADNAIKLA
                     VDMTVAGINRLNLQGNAEQQAAEVRAAMGVLFPEAVTLVREAREGFGTA"
     gene            21592..22395
                     /locus_tag="VF517_05180"
     CDS             21592..22395
                     /locus_tag="VF517_05180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052364.1"
                     /translation="MALPPPSEGSTALVTGASSGIGAELARELAGRGYGLTLVARRRD
                     RLQELARELKREHGVQANVSVCDLASPPARTRLLAAVKRRGLDVDILVNNAGFGSGGL
                     FHELDAESELTMVRTNVEAVLHLCTAFVPPMVERGYGSVLNVASVAAFQPVPRQVTYA
                     ATKSFVLSFTDGLTADLHGTGVSATSLCPGPVPTEFGEVAAIDDNLMSIPGVSVSPRD
                     TARQAIEGLDAGKRVVVPGPATRASAVIGRYAPRWLTLETMRRFYPVGK"
     gene            22392..>22878
                     /locus_tag="VF517_05185"
     CDS             22392..>22878
                     /locus_tag="VF517_05185"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8052365.1"
                     /translation="MSAATVEGAGVELAYDERGSGEPVVLVHGLACPRSLWHETVEAL
                     GDGARTIAYDRRGYGDSGVPEDYRRTTVEEQADDLIALLRALDAAPAVLCGLSFATLA
                     CLDAMAREPDLVRAAVLVEPPLLWLVQEGAEAMSQMRAALEEGAVRGGSAGAIDAFAA
                     HV"
BASE COUNT         3398 a         8206 c         7998 g         3276 t
ORIGIN      
        1 cggcctcggc gggcgcacga tcaagctcga ccccggcacc ccgggcgatc cgagcgacga
       61 catctcgacc accaccgcag gcgacggcag ctacagcttc cccggcctca agcccggcac
      121 gacctaccgc gtctacgacg agggcgagag cggctggacc tgctccgagc ccggcagccc
      181 ctgcgagtac agcgtcccga cgcagtccgg cgacggcacg gtcgagcgcg acttcggcgc
      241 ctggcgcccc gcgtcgattt cgggtgccac cttcgaggat cgcgatgccg acgccgcccg
      301 agatgccggc gagcccgccc tgacgggccg gaccgtctac atcgacgccg acggctcggg
      361 cggcctcacc tcgggcgacc cgaccaccac gtcgggcgcg ggcgggagct ggagcttcac
      421 cggcctgaag ccgggtgcgt acacggtgcg cgaggagctc ccctccggct ggacctgctc
      481 ggtgcccgcc ggcgcgtgcg cgcacgtgga gaccctggtc tcgggcgacg ctccgaccgg
      541 ccgcgacttc ggcagctggc ggggggtcac catcggcggc gtcaagtacg aggaccgcga
      601 cgccgacggc gatcgcgaca ccggcgagcc cggcctcggc gggcgcacga tcaagctcga
      661 ccccggcacc ccggccgatc cgagcgacga cctctcgacc accaccgccg gcgacggcag
      721 ctacagcttc cccggcctga agcccggcac cacctaccgc gtctacgacg agggcgagag
      781 cggctggagc tgctccgagc ccggcagccc ctgcgaatac agcgtcccga cgcagtccgg
      841 cgacggcacg gtcgagcgcg acttcggcgc ctggcgcccc gcgtcgctgt ccggtgtgag
      901 cttcgaggac ggcgaccgtg acgggacgcg cgacgcgggt gagcccggcc gcggcggctg
      961 gaccgtgacg ctcgaccccg gcacgccggc cgacgactcc gacgacgtcg acacgacgac
     1021 cgccgcggac ggcggctact cgttcaccgg gctgcggccg gacatggcct acacgctcac
     1081 gatcgagtcg cgcagcggct ggacctgcac cttccccgcg ggttcgtgcg accacgcgcg
     1141 cacccccgcc tccggcgacg cggtcaccgg gctcgacttc ggcgcgtacg ccgaggcttc
     1201 gctcgaggtc cggcaggagc tcgatcccgt gggcgacccc gggcgcttcg acctcaccgt
     1261 gggcaccgtt ctcgcgaagg cgggcgccgg ccacggggac tcgaccggcc gccgctcggt
     1321 cgccccgggc gaccacgccg tcacgagcaa ggccgcgtct ggcaccgccg gcggcgacta
     1381 cgactcgacc ctcgagtgcc gcgcgagcgg tggctcgggc tcggtcgtgc ccgcgccggg
     1441 cgggaccgtg accctcgcgg cgggcgacga cgtgctctgc accttcacca gcgtccgccg
     1501 cccggcgccc cccgccgatc cgcccgccga ccccccggcc gacccgcccg ccgacccgcc
     1561 ggtgcctccg gccgacccgc ccgcggaccc gccgaagccc cctctggacc cgcccgcacc
     1621 cccggcgccg ccggccgacg agtgcggtgc gaacggcgcc gacccggtcg ccccgggctg
     1681 cgagcagccg cccgctccgg agaccggcga ggcgtgcctc aagcgccccg tcctcacctg
     1741 ggtccgcgga agcggcataa ggcgcgtcgt cttctacttg gacggcaagc gccgcgcgac
     1801 cgtgatgcgc cccgacggcg agggccgcta cgccctgcgc accgatcgcc gcacgctcac
     1861 ggccggcaag cacgaggtcc gcgcgcaggt gttcttcagg cgcgccggcc gcgctccgaa
     1921 gacgctccgc ttcacgatcg agccctgcct gaagctcgag acctcgagcg ccatcgagac
     1981 gaccaggccc ggtgagctcg acgtgtgcgc cggcggcgcg ttccgtgcgt tcgtccgcgg
     2041 cgacacgatc cggcgcgtgg tcttctacct cgacggcaag aaggtcaagt cgacaaaggt
     2101 cgccgactgg cgcgggcact actgggtgga cgtcaggccg agcgcgctgt cggtcggcgg
     2161 gcacgagctg cgcgcgcgca tgtacttcat cagcggcagc aggcagcgct cgcgcgagct
     2221 gaagctcggc ttccggcgct gcggcggcta gccgggcgca ggactcgaac ggccgatctg
     2281 gaacgtggag ttgccgatga ccgcccgcct gcgactgatc gttctcctcg gccttgtggc
     2341 gttgctcacg cccgccgcgg cggcgcgcgc tacggagaag ccactgctcc ctccggaggt
     2401 gctgctcgcg caggagggga cgcccgcgcc gcccgccacc gaccaggagg acgaggagct
     2461 cggcgaggag atcccggacg aggacggctc cgacccgaaa ccgccccccg aggacgacga
     2521 ctcgggctcc gagggcacgg gcagcagcgc cccgccggcc gacccgccgg cgcccgagac
     2581 ccgcgcggcg accccggtcc gcagccttcc gcgcaccggc caggacccga ttccgctgct
     2641 ggtaaccggc atgttcctgc tcggaaccgg cctcgtgctg cgcgccgcag ttccccgcac
     2701 gtaacctccc ggccgccatg cgggcggttc agatcaacga cttcggcggg ccagaggtgc
     2761 tcgagctggt cgacctcgac gtgcctgagc ccggcgaggg gcaggtgctc gtcaaggtct
     2821 cccgcgcggg cgtgaacttc gccgacaccc accagcgcga gaacgcgtac gtgaagaagt
     2881 tcgggctgcc gctggtgccg gggaacgagg tggtcgggac gaccgaggac ggcacccgcg
     2941 tggtcgccat gaccggtggc gcgggcggct acgccgagta cgcgctcgcg ccggccgaca
     3001 gcgcgttccc ggtccccgac ggcgtttccg acgagacggc gctggcgctc ctcatccagg
     3061 gtctcaccgc gtggcacctg caccgcacct gcgcgcggat cgccgagggc gagagcgtgg
     3121 tggtgcactc ggcggtcggc ggggtgggca gcctggccac gcagctcgcg aagccgatgg
     3181 gggctgggcg cgtgatcgcc acggccggct cggaggagcg ccgcgcgaag gcgctcgagc
     3241 tgggcgccga cgccgccgtc gaccccgacg aggaggacct caccgccgcg ctgatcgagg
     3301 cgaacggagg cgagccgctc gacgtggtgc tcgagacctc cggtgggcgc gtcttcgacg
     3361 cgagcttcga ggcgctggcg ccgttcgggc ggctcgtggt ctacgggatc tcgagccgcg
     3421 agcagaacga ggtccgcacc gggaagctgc tccgcggcag tcgcagcgtg atcggcttct
     3481 ggctcatgca ttgcctcgaa cggcccgagc tgctgcgcga gccgctcgcc gacctgttcg
     3541 atcgggccgc gcgcggtgag ctgcgcgcgc tcgccggtgg cacctatccg ctctccgagg
     3601 tgaggcgcgc gcacgaggac cttgtgggcc gtcgcaccac cggaaagctc gtgctggacc
     3661 cgaccgccta ggctcccccc gtgacgaagt tcgcagacct gcgcctgtcg gagccgctgc
     3721 tccaggcgct ggcccacctc ggctacgacc agccgactcc gattcaggag caggcgatcc
     3781 ccgagttgct cgacggcaac gacgtgatcg gtcaggccca gaccggcacc ggcaagaccg
     3841 ccgcgttcgg cctcccgctg ctcgagtacg tggacaagga catgcccgag gtgcaggcgc
     3901 tggtgctcac tcccacgcgc gagctgtgca tccaggtgac ccaggcgctg cgcgcgtacg
     3961 gggagcgcaa ggggatcgag gtggtcgcgg tcttcggcgg cgcgccgatc cgcgaccagg
     4021 ccgcgttcct gcggcagggc gcgcaggtgg tggtcggcac ggtcggccgc gtgatggaca
     4081 tgatcaaccg ccactacctg ttcctcacgg aggcgcgcta cgtggtgctc gacgaggccg
     4141 acgagatgct cgacctcggc ttcctcgagg acgtcgagac gatcctcggc cgctgcccga
     4201 gcggccgcca gaccgcgctc ttctcggcga ccatgccgcc ggcgatcaag aagctcgccg
     4261 agaagcgcat gttcgacccg atcacgatca aggtgcgcgc cgcgcagctc acgatcgaca
     4321 cggtcgagca gttctacgtc gaggcatcgg accgcgagaa gcccgacgcg ctcgcgcggg
     4381 tgctgaaggc cgagcgcccc gaccaggcga tcatcttcgt ccgcacgaag atcggcgccg
     4441 accggctggc gcgccggctc ggcgacgacg gcatccgggt caaggcgctg cacggcgaca
     4501 tgtcgcaggg ccagcgcgac ggggtgatga tcgcgttcaa gggcggtcgc gagcggctgc
     4561 tggtggcgac cgacgtcgcc gcgcgcgggc tcgacatcaa gggcgtctcc catgtcgtca
     4621 actacgacat cccgaactcg cccgacatct acgtgcaccg catcggccgc accggccggg
     4681 tgggccgctc gggccgcgcg atcacgctga tcacgccgaa gcagcgcagc gacctcgacg
     4741 ccgtcgagcg ccacgcgaac accgagatcg aggagtggtc gccgggcgta gtcgcgagcg
     4801 cggaggccgc cgagcaccgc gccgactccg ccgcccaggc cacgagctcc ggcgactccg
     4861 aggccgccac gtcggtggcg cgcgaggcga aggccccggg ccgggatgcc gcgccgaagc
     4921 cgggggcccg cgaggccgct ccggccgccg ccgagccgga gcccgccgcc ggcgaagccg
     4981 cttcgagcgc tgcggatgcc gcgcccgcgg ctcgcgaggc cgctccggcc gagtccgctc
     5041 ccgccaccaa cggcgacggc gccgagcagc gcccgcgccg ccgccgctcg cgcggcgacc
     5101 gcgagacccg ccgcccgcgt cacaccaagc cgcgcaagga cccggacggc acgccgcgct
     5161 ccaagctgct cgtcagcgcc ggccgcgcgc acggcctcga gccggcggac ctgatccggg
     5221 ccatcgtcga gaatgcgcgg ctcgacggcg aggacgtcac cggcgtccgc gtgctcgagc
     5281 gcttcagctt cgccgaggtc ccgaccgagc acgccgacga ggtcgtgcgc agcgtcgggg
     5341 gccgcagcgt caagggaaca gagcttcgaa tggaggtaac gcgaacgtga gtcaggccac
     5401 gcttcacacg aaccacgggc cgatcgaggt cgagttcttc gacgaggacg cgcccaagac
     5461 cgtagagaac ttccgcaagc tggcgggcga cggcttctac gacggcctcg tcttccaccg
     5521 cgtgatcaag gacttcatga tccagggcgg ctgcccgcag ggccgcggca ccggcggccc
     5581 cggctacacg ttcgaggacg agttcaacca gcacaaggtc gagcgcggcg cgctcgccat
     5641 ggcgaactcc ggcccgaaca ccaacggcag ccagttcttc atcgtcacca ccgaggcggc
     5701 gccgtggctc gacggcaagc acaccgtctt cggccgcgtg acgtccggga tggaggcggt
     5761 cgacgcgatc gagaacacgc cgacgggcgc cggcgaccgc ccgcgcgagg acgccgtcat
     5821 cgagcgcgtg gagctgcccg aagcctaaaa actggctgcc cggccaactc gaccgttaga
     5881 ctccgcggac caccgccgga gtgccagcag aggagagaga atggccgtcg agtcgcaggt
     5941 cagcgaggca acgcagaacg ggcacggcaa cgggaagacg ttcgccgtgg agaaccccgc
     6001 cacgggcgcg acgatcgccc acctgcccga catggacgcg gcccaggtca aggcgctggt
     6061 cgaccgcgcc cgcgcggcgc agcccgcctg ggaggccctc ggcttcgagg gtcgcgcgcg
     6121 ctacatgttc cagttccgca agtggctcgt ggacaaccgc gaccgcgtcg cgcgcacggt
     6181 catggacgag accggcaaga cccgcgagga tgccttcctc gccgacgtct tcaccaccgc
     6241 cgacgcgctc ggcttctggg caaagaaggc acccgagtac ctgaaggacg agcgcgtgcg
     6301 ctcgaccaac ccgttcgtgc tcggcaagaa ggtcgtggtg cgctaccggc cgttcggcgt
     6361 ggcgggcgtg atcgggccct ggaactaccc gatcgccaac tgcttcggcg acgcggttcc
     6421 cgctctgatg gccggctgca ccgtggtcat gaagccgagc gaggtgaccc cgctggcctc
     6481 gctgctcgtg gaggagggcc tgcgcgagat cggcctgccc gaggacgtgt gcctggtcgc
     6541 caccggcacg ggcgccgcgg gagcggccct ggtggacgag gccgacgtcg tcatgttcac
     6601 cggctccacg gccacgggga agaaggtcgc cgcgcgcgcg gtcgagtcgc tcacgccgtt
     6661 ctccctcgag atgggcggca aggacccgat gatcgtgctg cgcgacgccg acgtggagcg
     6721 cgccgtcaac gaggccgtct actggggcat ggccaacggc ggccagatct gccagtcggt
     6781 cgagcgcgtc tacgtggagg agccggtcta cgacgagttc gtggagaagg tcgtcgagcg
     6841 cgtcaaggcg ctcaagcagg gtccctcgga cgggcccggg tcggccgaga tcggcgcgat
     6901 caccttcgcc ccgcagcagg agatcatcga gcgccacatg cgcgacgcgg tggacaaggg
     6961 agccgagatc cgcgtcggcg gcaaggccgc cgagaagggc gccggccgct ggtgggagcc
     7021 gacggtcctc acgaacgtcg accacacgat ggagtgcatg accgaggaga cgttcgggcc
     7081 gacgctgccg atcatgaagg tccgcgacga gcacgaggcg ctgcgcctcg ccaacgactc
     7141 gccctacggc ctgaactcga gcgtgtacac gcgtgacatc gagaagggcg agcggatcgc
     7201 gcgcgggctg accgcgggca acgcctgcgt gaacgacacc gtcgtgaact acgccgcggc
     7261 cgatgcgccg ttcggcggca cgggcgagtc gggcatgggc gtgcggcacg gcccccaggg
     7321 catccgcaag tactgcggcg tccagacgat cctcacgtcg cgcttccaca tgaagaagga
     7381 ggcgtacatg ttcccgtaca gcccgatgaa gtcgaagttc gtggagcggc tgtcggtcac
     7441 cctgtacggc cggatcccga ggaggtaccg ctagatggcg acgcgacgcg cggaggcgac
     7501 ctgggagggc agcctcgccg agggcagcgg cacgaccgag ctcgacagcg gcgtggcgcc
     7561 cgcgctcgac gtgaactggc gggcgcgcac cgagggcggc gcgcagacga gcccggagga
     7621 gctgctcgcc gcgtcgcacg ccgcgtgctt cgcgatggcg ctctcgcacg agctgtccga
     7681 ggcgggcaac cagccgcagc gactgacggt cggggcggcg gtctcgttcg gtccggtcga
     7741 gggcggcttc gcggtgacga agtcggagct caaggtgcgc ggcgtggtgc cgggcatcga
     7801 cgccgatgcg ttcgagaagg ccgcgcaggg agccggcgag ggctgcccga tctccgccgc
     7861 gctcaagggc aacgtcgaga tctcggtcga cgcccagctc gagtcggctt agcgcagccc
     7921 gggtagccgc cgcctcgccg ccgtgccggc cgcgcgccgc agcgccttcc cggccgcggc
     7981 cgcgcttccg tgcccggccg cgcgctgcgc ccctcgcggc ccccgcgagc gcggttgctt
     8041 ggatagggcg catgggcgcg atagcactcg gcttcgcatc ggcggtcgtc tggggcgcgg
     8101 cggacttcct gggcgggctc aagagcaggc aggtcgcgct gttcgcggtg atgctgctct
     8161 cgcaggcggc cggcctcgcg ccgctgctga tcgcggccgc cgtgcgcggc gagccgccgc
     8221 cgggcgccga catccttccg tggtgcgtgc tgggggcggt ggccgggacg gtcgggctcg
     8281 cggcgttcta ccgcgggctc gcggtggggc agatgggggt ggtcgcgccg atttcggcga
     8341 gcgccgcggc gatcccggtc gtgttcggag tcgcgagcgg ggagcggccc ggcgcggtcc
     8401 aggtcgccgg catcgcggtg gcgatcgtgg gcgtggcgct ggcctcgtgg gagccgcgcg
     8461 aggaggagcc cccggcccag cgcgcggccg ccgaggccgg cgaggcggag caccccgagg
     8521 ccggcggggc ggagcacccc gaggccggcc gggcatggca cgccgagacc ggggcgcagc
     8581 accccgacgc cggcggggcg cagcacgccg tcccccgccg aggcgtgggc ctcgccaacg
     8641 catccaccgg cgccggcctc gcacttgtcg ccgcggtcgg cttcggcctg ttcttcgtcg
     8701 ggctcgacga ggcgagcgac cgcgacctcc tctgggcgct cgtcctcacc cgctcggtct
     8761 cgaccggggc gctcgtgctc gccgccctcg cgacgcgccc gaacctcgcg atgacgctcg
     8821 cggacgggcg gacgatcgcc gcggtgggca tcctcgacgt cagcgccaac gccctgttcg
     8881 cagcgggcac cacggtcggg ctcgtgagcg tcgtgtcggt gctcggctcg ctctaccccg
     8941 tcaccacgat cgtgctcgcg cgcttcctgc tcggcgagcg cctgggcccg gtgcagcgcg
     9001 gcggcgccgt cacggccctc gtcggcgtgg ccctgatcag cgcgggctga gcgcggcgcc
     9061 cgcgccggct agcttcgcga cagcagcgcg atgccggcgg cggtgaccgt cgaggcggcc
     9121 gtgaccccgg ctgcgcgccg gcccggcacg agctcgtccc actcgaggag ctgaaggccg
     9181 gcgtccatcg cgtcggcggc gatgccggcg gcgagccacg gccggacgtc gctcccgccg
     9241 agggcggcgt agaccaggcc gccgccgagg atcaggtcgc gggtgccgta ggagcgcgcc
     9301 gcgaagcgcg cgccggtctg ctccgccgcc gtgcccatca gcagcccggc catcggccgc
     9361 ggcacggtca tcagaccgat tccgaacgcg atacgtccga caccgagcgc gagctggagc
     9421 gagcggatca cgccgccagc tccaaaccga gcaggcgctc gagctccggc accgtcctgt
     9481 cggcgccgcg gtggaggatc ggggtcatcc cgaccgccgc cgcaccctcg cagttctcgc
     9541 gcaggtcgtc cacgaacacg cagtcggccg gcggcacacc gatcttgtcg gcgccgaggt
     9601 ggaagatcgc gggctcgggc ttgtgcagcc ccacgtcgcc cgagatcacg gcggcgtcga
     9661 acagctcgtc gatgagctgc tcgtcgtagg tcgtgcggcc ccaggagttc gagatcaggc
     9721 cggtgggcac acccgcggcc cgcgcgcggc ggacggcgtc gaccatccgc tcgtccggcc
     9781 cgactcccgc gaacagccgc tcgaccatgc cctcggtccg ctcgatgccc agcacggccc
     9841 cgaacttcgg ctcgaactgc tcgaccgtga gctcaccgcg ctcgagctgg cgcagcaggt
     9901 cgagcccctc gccgcgcgag cggaacatct ccttgaccgt gttcggctcg agcccctcgg
     9961 cctcgcagaa cgcgcggaac gactcgaaga cgttcgtggt gagaacgccg ccgaagtcga
    10021 cgagcaggcc gcggctcacg tgctcggcgc cgacatctgc gtgatcccga gcgctgcctg
    10081 cgcgagctgc ggcacgccga cgtcgaacag cttcaggtac gggtcgtcgg tcgcgcccgc
    10141 gagcgagcgc ttgtagttgc cctccatgaa caccgccgcc ttccagagtg ccagcgcctg
    10201 gtaccagcgc aggtcgctca tcgagcgccc gctcgcctcc tcgtagcgag ccaccagttc
    10261 gtcgcgcgag aggaagccct cctcgtgcgt gacggtgagc atgtcgaaca tcgtcggctc
    10321 gcggtcgccc ccggccggct gcgaccaggt ggcggtgagg tagcccacgt ccgccagcgg
    10381 atcgccgatc gtcgccatct cccagtcgaa gatggcgatc agcctcgcgg gggcgtcgct
    10441 cgcgaacatc gtgttgccga ggcggtagtc gccatgcacg atcgtcgcgg ccggggactc
    10501 gggcttgttg gcggcgagcc attcgccgac ctcctgcacg gccggcagct cgcgcgtctt
    10561 gttgatgtcc cacaggccgt tgaagcggcg cagctggcgg tcgaggtacc cggttggctt
    10621 gccgaatccc tcgagcccac aggcccgcca gtcgacggcg tgcatctcgg cgagcgcgtc
    10681 gaccagctcg aggcagatcc ggcggcgctc cccggggctc gacagctcct ccggcacctc
    10741 ggagacgatc accgcgccct ccaggtactc catgacgtag aacggcacgc cgagcacgga
    10801 ctcgtcgtcg caggcggcca gcaccgtcgg cgtgcgaacc tcggcgctct cgatcgcgtc
    10861 gagcagccgc gcctcgcgaa gcacgtcgtg cgccgacgga ggcagcggcg ggcgcggcgg
    10921 cctgcgcagc acgaagcgct cgtccccgcg gcggatcagg taggtcacgt tcgagtggcc
    10981 ctggccgatc ggctcggcct cgaccgggcc ggagccgagc ccacgggaat cgaggtactc
    11041 ggtgagcggg tcgaggacga tcagcggcgg gcgctcctgc gcccgggcct cgtccggggt
    11101 ccgtacgaca tccatgggtc gggcatcttg aaccatcggg ggccgagcgc gtataaaggg
    11161 cacagatgcg ccgtctcgcg attgccctcc cgctggccct ccttctgtgc tcaccggcca
    11221 tggccgccga ccagagcgtg agcgccacga actcgaacac gttcacaccc agcacggtca
    11281 cggtgaaccc gaacgacacg gtcacctgga ccaaccaggg cggcttccac aacgtggtct
    11341 tcgacgacgg gtccttcacc gatccggcgc agcccagctt ctcgatgtgg accgtgcaac
    11401 gcaagttccc gacggcgggc agcttcaagt accactgcgg ctaccacggg accagcatgt
    11461 ccggcaccgt cgtcgtccag gccgctcagc agccgccggc cggcggccag cccgacacca
    11521 cgccgcccga catcgacgac ctgaagctcg tgccggccac cttctgcaac aagaagacgt
    11581 cgaagtgcaa gagggtcggc acgctgatcc gcttcacgct agacgaggac gcgaagatca
    11641 gcgggcgggt ggtgaaccgc aagactggca agaaggccgg ctcggtcacg atcagcgcga
    11701 gcgccggcgc gaacgagttc gactactcgg gcaagggcct cgcgctcggc cgctacaggc
    11761 tcgagctcac cccgcgcgac gccgccggca acaaggccgc gaagcccacg cgcgcgaact
    11821 tcacggtcgc gacgaagcgc tagctgtcct gccccggacg gtccgaacct agccttcgtg
    11881 ccggcgctca tccgcctgga caccggcgat gctggcagcc accgcgtcgg tgacctcctc
    11941 ggcgctgctg ctgccatcga gctcgagcgt gatgactgag gcggcgagcg cgtccaaccg
    12001 gacaaagtat gcggcccgga gagcctcgag gtaagtgatt gcgagaccct gctcgaactc
    12061 acgtccgcgc gcgcggatgc gtgcgaggca gacctttggt ggtacgtcga ggaagaccgc
    12121 gatgccaggc cttgccaggt cctcccagac gtgcgcctcg aagacctcaa tgaggcatgc
    12181 gtccttgtcc gaaacgttca cgctcgcgaa aacggatgtc tttgccgggg tgaagtcgga
    12241 caccaccgac tcgcccggct cgactgcctt cgcctcgtgc agccttaggg ccatgaagac
    12301 gagctcggtc tccagtgcga accgatcggg ttcctcgtag aagcgcggga gcagcgggtg
    12361 tgctgctacc cgctccagca gcggctgagc ccctaggcgt tccgcgagca ggttcgcggt
    12421 cgtcgtcttc cccgctccga tcaggccttc gacggcgatc caggccccat cagtcacggt
    12481 cgactcggcg ggttacatgc ggagcgaacg cggcaacgcg cgtgtgggag aggctgagac
    12541 gctggatctc gtggctgcgc aggcgacgct cctccgcaag gcgcgcgagc agcttccatc
    12601 gaaggtcgag cagcgccagc cgcacccgcg cgcgcggtga ctcccaccgc acctccagct
    12661 gagggagcag gaggttcatg tgcacgccag ggtaaaagcg ctcgtccgtg acctcgcagt
    12721 ggcgcgtctt tgcaacaacg ccgttgcgta cttcgacgta cgcggtgtcg acggaatggc
    12781 gggtcccagc gaaggaacgc agttcgtcct tcgccgccgg cttgtcgccg cggtactcgg
    12841 acagcgtgcg cgagaatccg tcgtgcagtg cgtggatgtc gcgtagtgcg aggtagttga
    12901 agtcgtcgag gtcgaaagcg tggagggcga gcgagtagaa gcccgggagc ccgtggtagg
    12961 ccggtccagc gtggctcggc gccggccaca tgctgagctt ctgctgaaat gcgcagcggc
    13021 cgtgctcgcc tcgcacctca ctgaagtacg ccacacggtc gtggaagaca tcgattcctt
    13081 taggatcgat gaacgcgagc tcggacatcg cagtcacgat gtgaggtagg tcgtcgtagc
    13141 tcgggatgac aacgaggagg tcgaggtcgg agggacgctc gcggacgctc cccgcggtgt
    13201 tgtagaagcg tccgtagctt gccgatccgc ctaagatggc gctcgtcgag agtcgctcga
    13261 gccgatcgcg cacctggttg aggtcggcga tgcgacgaac ggtttcgacc gccgcctcga
    13321 acggacgcgg ccttcgtttc ccgccggtgt gcgcgcggta agcggggaaa ttctccagcc
    13381 gtatgtcttt ataaaggaac cagaatcgct caggcggggg ggagtcggaa taaccgtcga
    13441 tcgcccccat aagctgtgcc agatcggtga cgaacgaggt ctcgtggtct tcgtacgatg
    13501 cgaacgtacg cgtgagcgcc tcagccagtc cgcccgccag atcggtgggc ggctcaacgt
    13561 ccggcgcgat tgccgaaaat tgcttgtaca ggccgcggat acgccgccag tcgccgcgct
    13621 cggagattgt cgccatgacc gcgtcgatgt tgctcatcgg cgtccagcgc cgtccgcgat
    13681 gtcgaacaac ggaagctgat cggtcagctc tttccgaacc gcatcgctcg ccacgtcttg
    13741 gaagcgctcc cactcgccga cttccaacag aggcctgcag acggagggcg acaggaagat
    13801 gcgcgccggc cgtgcggagc gttgaaacgc ctgcgtcacc gcgtgagcga gaggagtcag
    13861 gtcctgtacg ggggcggtcc gcttctccgt cccgcgttcc acgagcacgt agaggtcgcg
    13921 gacctcgtcg cggcccgcca gcggaatatc gaggaggacg tcgtgcgtat cgagcttgac
    13981 ttgcgacttg atttgaggcg attcattcgc ctcggctgtg atcgcggcgg caacgttgct
    14041 gcggaggcga cgccggctga gaaggaccgc gacgccgtcg gcccgcgccc cccgcgtcca
    14101 gtgttgctgc agcgcgtcgt acaccttgtc gtcaacgggc ttggcgtctg ccagctcgtc
    14161 gagcaactcg gtgcgcggct ggaactccgc aacgtgccaa aagacgtttc ttcgattgcc
    14221 acgcacgccc tcgcacaact cgcggacatg cttctgtcga cgggcgagct tcgcctccag
    14281 ccagtcgagc gcagcgtcgt ccgacaattc gcggaactgg tggaggagat cgcggagacg
    14341 tgacgcaacg aacttggagt cgtcgctcga caccacgtgc tcttcgaccg cctcctggag
    14401 catgacggtc atggcgcgga cagtgtgctg ccaatagacg gcgcggaaca tctggtagcg
    14461 cgcgatcagc atgctctcga gcggcagtac gcccttctgc gtcacgccaa tggtgcctcg
    14521 caacacaccg tcgatcggga cctcgagcgt ggtgagcgcc tgatcaagac gacggcggtc
    14581 gattccgctt gcgtactcga caccgcaatg gtgggcgtca cgcacgaggt agtctgcctt
    14641 gtccgcgtcg agctggccgt cgatgagcga ccggagggtc tcaaggtgca gtcgctgcga
    14701 ttgtgccgtt ggatcctcgt ctgccccagg agtcccggca cccgcctcta gtatttcgat
    14761 ggctctgcca accagctgct ggcggctcat gtcgctcgac acccagtatt ggtcgagcac
    14821 ctcgacgagc gcttctgcgt ccttgccgat cgcgtccgtc ttccgtctgt cgccgatggt
    14881 ggcgcgtagg accgcccggc catagctctc gtggtgctcg ttggggggga tgagcggcga
    14941 ctcttcgagc tggtgcccgt acgctgggtg ggcgagatcg tggacgagtg ttgcgaggat
    15001 cagcgccgta acgtcgagct tcgacgtgtc gagcctgata ctgaccgtcg aacgatccgc
    15061 gaacagcgcg cggatccgct gaagaagctc gactagcgcc ccactgacgt gctcccagcg
    15121 ggtgtgctca gcgcccggga acacatcgcg gacggtggcg agctgccggt gccgcttgag
    15181 ccggagcagc ggtctgctgt tcatcagcgc attgacgcgc ggcgtccacg gaacgttgtt
    15241 gaagggcggc acacgcagga cgtgttggga cacggcctca agttccggca cgtccgtcgc
    15301 ctcaccgtac tccggcaaca gacgctcgag agcccgcacg acggcatccg cgtcctcgta
    15361 gaagggagtc gcgtccggcg agtcggccgc caacagcctt ccgacaagcc ttcgcaggta
    15421 cgcgcacacg aagccgccag cctcggaccc gaatatcgag tcgcgccgcg cggcgcaaag
    15481 cgccacggcg tcgacgccgt ctgcgggatc gtcgagcgcc gcactcgcct cgtcgaagcc
    15541 gaacacgcgg ttcacttcgc gcgcgagcat gtagaggtcg agccatggcc tgtcccaggc
    15601 cccctcgccg gctttgaaat ggcacggggc gcgattgttt gagacgcgct tgcccgccgc
    15661 ggtgagattt cccctgattt ttagtgcgcg cctctcgagc tcgggcggca agttcagcag
    15721 cgtggtctcg gcctcgtatc gcgtctcgta cttgtctcct ggcgggcgca gcggtcgcgc
    15781 attcccgagg tccatgagct tcaccacccc ctgtgccgag acgagaacat tgtcgccctt
    15841 aacgtcccaa tgaaccttgc ccgactcgtg cagttgcacg agggcgcggg aggcctgttg
    15901 aatgaggtcc gcgacctgcc aggcgtccgc tgcaccttcc tccaggtatc gcccaaacgg
    15961 ctgcgcccct tggacccact cgatcagcgt cgcgccgagc ttgaggtcgt ggacctgaga
    16021 gcgcttcacg atgaccgtgt ccgttccgta cagcttcgcg atgttctcgt gcgacagggg
    16081 caggtgcgtt cgccgctcgt cctccgcctt ctcgtactcc tctgagatct cgccccgctc
    16141 gcgggcctct tcatagagag ttggccgtac caccttcagc gcgtaatcga ccggcacgtc
    16201 cgcctcgcgg caggggatca ctacgccgcc gcctccgacc atgatctgca cgttccgcag
    16261 cggcagctcg agtcggtgac cgcatagaga gtcgccggtc ggagaccgca gcgcgcgatt
    16321 gcggttcccg accttgaacg taaagttctt gcccaggaac tcgtcgagct cggcgggggc
    16381 atctggccaa acggccctga gcacggcgac ggcgtcgtcg cgccgagctt gctcgcgttg
    16441 ggcgtcggtg gtcatggcaa taaccgagga ctgcagcgct ctcgccgcga cccgcgcacc
    16501 aaacactaat cgagcagatc gaccaccgca atttctgcga acatcacaaa ttgagcaaaa
    16561 gtgcgattcg gtccggcggc gccctgacac ccacgaggtc taacgagcga tccagataac
    16621 aaccgcagcg caaagacgat gccgtgaagg cttagcaccg tgacgctgaa caaggcccga
    16681 accagtcccg aaggttacct cggctgcagt cacgctgtaa tcgattgaac tcgtcgtcct
    16741 cgtcgtttcg cagacggccc ggtaacgccg gtcgacggcc aaatagtagg cgtcgagcat
    16801 tcgccgctgg tatcgctctg atcacgggat tgccgcaaag gatcggtcga tgtctgcgac
    16861 cgcgaggggc catcaacgcc gctgatgccg gcacgccagc ccttggacag aaacgaattg
    16921 ctccctttcg ctcgatgacg ccctgaaaat catcttgagg cgatcacgtc cggcatgaat
    16981 tcggcgcaag cgctagccgg cgatctcgtc gacgtggcgg atgttcgccg cgtcgtcgtg
    17041 gtccgggctc ggctcgccct cgaaccacgc gtcgaggatc tccccgagca gcgcctcgct
    17101 ggtcgtgcgc agcgacagcg ccagcacgtt cgcgtcgttc caccggcgcg cgccgcgggc
    17161 ggtctcggcg tcggcgcaca gcgcggcgcg cacgccgtcg accttgttcg ccgcgatcga
    17221 cgcgcccgtg ccggtccagc agcagacgat gccctgctcg gtgcgaccgc cggccacgtc
    17281 gcgcgcggct gcctcgctgg cccacgccca gtcgggccgc tcgccctccg ccagcgcgcc
    17341 gtgcacgacc ggctcgtgcc cgcgcctgcg cagctcctcc acgaccgcct cggccacgcc
    17401 cgtgcgctcg tcgctcgaca ccgcgatccg catgccgcca gggtagggcg ccagatcgga
    17461 tgacacgcgc gtcatccgcg actaccgtgg tcgtactcgt gctgacgcgt cgctggcccc
    17521 tcgcagtcgc actcgccgcg ctcatgctcg gcgtgctcgc cacgccctcg ctcgcggccg
    17581 tgcagacgaa gtcgttcgac tacccggtcc agatggaccc gttcgaggtc cagcaggcga
    17641 ccgcgatcgt caaccccggc atcgagggct acgtgaccgg gatgagcgtc gacgtggtga
    17701 acgccgacgg cacgcaggtc ccgatcaacc gggtgatgct ccaccacatc gtcttcgcgc
    17761 ggctgggcgt caagcacccg ggctgtgact cgatcaccca gttcgacagc aagaccaaga
    17821 tcccggccgc cggcgagccc atgtacgcgg ccggcgagga gcgccaggtg ctcgccctgc
    17881 cgcccggcta cggctaccac atgacggcga acgcgccgct ctacatggtc tggatgctga
    17941 tgaaccaccg cggcgtgaag gacaacgcga acatccgcta cacgatcacc tacgaggact
    18001 ccgagcagct caagccggtc cggccgctct ggatggacgt ggcgaactgt caggccgacc
    18061 ccgtctacaa cgtgccgggg gacggcaagc ccggctcggt ccacgagcgc acctacgacc
    18121 tggtcgcgcc ggagtccggc cgcatcgtcg ccggcggcgg gcacgtgcac ggcggcggca
    18181 tcgagaccgc gatcagcgag ccggactgca acaaccgcac ggtcatggca tcgaagcccg
    18241 cctgggggat gcccgaccac cccttctaca aggtgcgccc gatcctccac gagcccgggc
    18301 ccgtgtcgat gagcggctgg ctctcggggc agggttaccc ggtccagaag gggcagcgca
    18361 tccggctcac gtcgcgctac gacaacagcc gcccgcacac ccgcgtgatg gggatcaacg
    18421 tgatctacct cgcgccgggc gaggtgaacg agccgtgcgg cccgccgcct accgacggcg
    18481 tcaacatcca ggccgccgag ctgcgcggcg agccgtaccg gaccaagacg ccgaagttca
    18541 cggtgccgct gacgggcgtg gactccaagg gccgcgccgt gaccatctcg cgcccgcccg
    18601 ggaagacgcg cgcggtcaag agcgggacga cgatcaaggc gcgcgactcc tacttctcga
    18661 tccccaacgt cgaggtgaag aagggctcga agctcagctg gcagttcggc acgccgacgc
    18721 tgcacaacgt cacgctcgcc aacggcccac gcggcttcgc gtccgacaac ctcaacgagg
    18781 gccgcaagtt caacatgaag ttcaaggtgc ccggcaccta ccggctgatg tgcacgctgc
    18841 acccggtcgg catgacgcag accgtcaccg tccgctagcg ggcggcccgc cctaacctcg
    18901 gctccatgcc ggagctgccg gaggtcgaga tcgcggtgcg gcgcctcgac gccgcgctcg
    18961 agggtgcgcg cgtggagtcg gcgctggcgc cggggatggt cacgatgaag acgttcgacc
    19021 cgccgctcga cgcgctggcc ggtcgcacga tcgagcgcgt gcggcggatc gggaagatgc
    19081 cggtggtcga gttcagtggc gacctcgccc tcctcgtgca tctgatgtcc gcggggcggc
    19141 tgcagctctt ccccgaccgt gcgtcgctgc gagacaagcg ctcgcggctg ctggtccgga
    19201 tcgacgacgg gcgcgaactg cggctgcgcg agttcggcac ccagcagcga gcgtgggcca
    19261 agctgctccc gctcgcggac ctcgccaccg acgaggcggt ggccaagctc gggccggagg
    19321 cgtacccgcc gctgccgctc gaggagttcg cgcggctggt cgaccagccc caccacctcc
    19381 acccgatgct gcgcgaccag cgcaccatcg ccgggatcgg ccgctcgtgg gtggacgaga
    19441 tcctctggca ggccggcctc tcgccgttcc gcaagggcaa cgacctcgag cccgaggagg
    19501 tcgagcgcct gcacgccgcc gtcggcgacg tgctcggcgg agcgctcgag cactacgaga
    19561 aggtcgtcgg cgagtcgttg cccgacaagg cgccgatgcc gctccaggtg caccgcaagc
    19621 ccggcgagcc gtgcccgcgc tgcggcgcga cgatcgaggc ggtccacttc aaggactacg
    19681 tgatgtgtta ctgcccgcag gagcagaccg gtgggcgcgt gctgaaggac cggcgcctgt
    19741 cgcgcctcct caaatgaggg agcggctgct cgggcttccg cgctggcgaa tcgcgctcga
    19801 cgtggcgatc tgcggtctgt tcgcggggct gctcacgctc ggactgggcg ccctcggcga
    19861 cgccgagctc gacgcggtcg cgcgcgacgc gcttgcggtc gggatcgcgg ccgcgctgtt
    19921 ctacgcgttc gcggtctacc gcgtccgtcg cggcctgtga gggccctcgc ggggttcgtc
    19981 ccgcactgcc tgatcctgtt ccggcgcctg ctcgccgacg accgggtgtc ccgcgcgcgc
    20041 aagctgctcc tggtcgcggt ggtggcgtac ctggcgttcc cgctcgacct gatcccggac
    20101 ttcatcccgg tgctgggcca gctcgacgac gcgctggtgg tggcgctggc gctgcgcgcg
    20161 atcgcacgcg gcgccggggc ggagctgctg tccgagcact ggcccggccc ggacgcgagc
    20221 ctgcgcaccg tactgcggct ggcgggggcg cgcccgtcgc cgacgtaagg ttgccgcgtg
    20281 ccccctgtgg ccaccctcgg cgactccttt cggtcgttct tcgacgctgt cgaggagttc
    20341 ttttcgaacc tcgccgccgt gcactggggc tcgctcgtcg tcgcgctgat cgcgttcggc
    20401 gtgtacctga cgctgcgctc gcgcgcctcc tacaacatcc tccgcgcggc ctacccggag
    20461 gagcagttcc gctggcgcga gatctggggc gcgtacatgg ccgggtacgg cttcaacagc
    20521 gtgatcccgg cgcgcggcgg cgacctcgtc cgcctcttcc tgaccaagaa cgcgatcccc
    20581 aacgcgacct acccggcgac cggggccgcg ttcactgtcg agctcggatt cgaccttgtt
    20641 ttcggtggca cgatcctgat cttcgcgttc acgcagggcg tgttccccaa gccgcccgac
    20701 ctctcgaagc tgaacgcgtt cgagctctcc tacttcgccc agcaccccag gttcacgctg
    20761 tttctgctga cggcgatggc gatcggcgcg atcgtggcca ccgcgatgct ctcggcgcgg
    20821 gtgcgcgcgt tctggaccgg cgtgcgccag ggcctcacga tcgcgttcga ccggcgccgc
    20881 tacctgcgcg aggtgttcgc gatccaggcc gcgggctggg tcttccgctt cacggcattc
    20941 tggttcctgc tcgaggcgtt caacgtcggg ggatcggtga agaacgtgct gctcgtgctc
    21001 ggcgtgcagg cggtctcggc ggcgcttccg ttcaccccgg gcggggcggg cgcgcagcag
    21061 gcgctgctcg tgaaggtctt cggcggctcc tccacggtgg ccgcttactc ggtcggccag
    21121 cagatcgcga tagcggcgtt cacgttcgcg gtcggcttcg cggcgctcgc gttcatcttc
    21181 cgggtgcgca gcttcaagga ggtcatcgca cgcgggcgcg agcagcgcga ggaggaggcg
    21241 cgccgcaagg aggcggcggc ggggccggtc taggcggttc cgaagccctc gcgggcctcg
    21301 cgcacgagcg tgacggcctc ggggaacagc acgcccatcg ccgcgcgcac ctcggccgcc
    21361 tgctgctcgg cgttgccctg gaggttgagc cggttgatcc cggccacggt catgtccacg
    21421 gccagcttga tcgcgttgtc ggcccaggcc accttctgcg ctccctgcat ctgctcgccc
    21481 agctcctgca tccggcgccg cagctcctcc gggtcgccga acagccctcc gaacggatcg
    21541 tcttcagcca tgcgtccgat tgtacgggga ccggataggc tcggccgggc gatggcactg
    21601 ccgccgccaa gcgagggctc gaccgcgctg gtcacgggcg cgtcgtccgg gatcggggcc
    21661 gagctggccc gtgagctcgc cgggcgcggg tacggcctca cgctcgtggc gcgccgccgc
    21721 gaccggctcc aggagctcgc gcgcgagctg aagcgcgagc acggcgtgca ggcgaacgtg
    21781 tcggtctgcg acctggcttc gccgccggcg cgcacgcggc tcctggccgc cgtcaagcgc
    21841 cgcggcctcg acgtggacat cctcgtgaac aacgccggct tcggcagcgg cgggctcttc
    21901 cacgagctcg acgccgagag cgagctgacg atggtccgca cgaacgtgga ggccgtgctc
    21961 cacctctgca ccgcgttcgt ccccccgatg gtcgagcgcg gctacggatc ggtgctcaac
    22021 gtggcgtcgg tggcggcgtt ccagccggtg ccgcgccagg tcacctacgc ggccacgaag
    22081 tcgttcgtcc tcagcttcac cgacggcctg accgccgacc tccacggcac cggggtgagc
    22141 gccacctcgc tctgtcccgg gccggtgccc accgagttcg gcgaggtcgc ggcgatcgac
    22201 gacaacctga tgagcatccc gggcgtctcg gtgtcgccgc gggacaccgc gcgccaggcg
    22261 atcgagggcc tcgacgcggg caagcgcgtg gtggtccccg ggcccgccac gcgcgcatcg
    22321 gccgtgatcg gccgctacgc gccgcgctgg ctcacgctcg agacgatgcg ccgcttctac
    22381 ccggtgggca agtgagcgcg gcgacggtcg agggcgcggg cgtcgagctc gcctacgacg
    22441 agcgcggctc cggtgagccg gtcgtgctgg tgcacgggct cgcgtgcccg cgctcgctct
    22501 ggcacgagac ggtggaggcg ctcggggacg gcgcgcgcac gatcgcctac gaccgccgcg
    22561 gatacggcga cagcggcgtg cccgaggact acaggcgcac gaccgtcgag gagcaggccg
    22621 acgacctgat cgcgctgctg cgcgcgctcg acgccgcgcc cgcggtgctc tgcgggctca
    22681 gcttcgccac gctcgcgtgc ctcgacgcga tggcgcgcga gccggacctc gtgcgggccg
    22741 ccgtgctggt cgagccgccc ctgctgtggc tcgtgcagga gggcgccgag gccatgtcgc
    22801 agatgcgcgc cgcgctcgag gagggcgccg tgcgcggcgg ctcggcgggc gcaatcgacg
    22861 cgttcgcagc gcacgtgt
//
LOCUS       DASVPL010000104        15442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_248735, whole genome shotgun sequence.
ACCESSION   DASVPL010000104 DASVPL010000000
VERSION     DASVPL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248735"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..406
                     /gene="gatC"
                     /locus_tag="VF517_05190"
     CDS             110..406
                     /gene="gatC"
                     /locus_tag="VF517_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX8052366.1"
                     /translation="MIEREQVLHVARLARLELSDEEVERMSGELSSILDHIEKINELA
                     LDDVAPTSHVIGVENVLRPDEPRPSIPRERALELAPDATDDGFRVPPSRAPDAA"
     gene            403..1821
                     /gene="gatA"
                     /locus_tag="VF517_05195"
     CDS             403..1821
                     /gene="gatA"
                     /locus_tag="VF517_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX8052367.1"
                     /translation="MSGLLDLTGAQAAERIRAGETSAKELFDAYRERAAAEDLNAFLW
                     LADAAPDAEPAQDAPLGGVPLAVKDLFCVEGVPSQAASRILEGYRPPYTATAVERLTA
                     AGAPVLGKTNMDEFAMGSSNENSGYGPVLNPWDRERVPGGSSGGSAAAVAAGLAPWAI
                     GTDTGGSIRQPAALCGVVGLKPTYGAVSRYGMIAFASSLDQCGPITRDVTDCALLLKH
                     MVGRDPCDSTSLDFPEPVELPTLIRLDGMRFGVPPELSGEGIEPGVRQVFEDTLKLIE
                     ELGGTIEEVALPHSPQAISAYYVIAPAEASANLARYDGVRYGTRHGEGDLTSLYEETR
                     ATGFGDEVKRRIMLGTYALSSGYYEAYYGRAQRVRTKIADDFRNAFEKVDLIVTPTSP
                     TVAFRLGERTDDPLAMYLSDYCTVSMPLAGIPAISIPGGLAGGLPVGIQLAGPAFSEN
                     RILDAAHAIEQAVGFDGSSARG"
     gene            1814..3259
                     /gene="gatB"
                     /locus_tag="VF517_05200"
     CDS             1814..3259
                     /gene="gatB"
                     /locus_tag="VF517_05200"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX8052368.1"
                     /translation="MAEAALAERHYEPVIGLEIHVQLRTRTKMFCGCALSFGEEPNTR
                     TCPICLGHPGTLPVTNAEAVHYGIMIALALGCEVAPRSIFHRKNYFYPDLPKGYQVSQ
                     YDIPLGSAGRLGEVRVHRVHLEEDAAKLVHAGSSGRIHGAEASVVDFNRGGTPLVEIV
                     SEPDLRSAADAGEWARLLRATLRALGVSDVNMEEGSLRVDANVSIRPAGESTLGTKTE
                     LKNMNSFRFLERGIEAEIARQEAILRDGGEVHQETLHYDPQSGAISSLRSKEEAHDYR
                     YFPEPDLVPIAPTEAMMTRAREALPELPAARAERFESELGLPAATAKLLAFRGELGDF
                     YEETVAAADGGAEPAAIANWVVGELLARLGDVEPGDSRVEPAALAKLVAMVSEKRISG
                     AAGKEVLGVLADAGGDPEAIAAERSLGMADTSELDAIVDRAIEQNADAVEKIRGGADK
                     AIGAIVGAVMRETKGRADGGEVQRMIRERIG"
     gene            3349..3573
                     /locus_tag="VF517_05205"
     CDS             3349..3573
                     /locus_tag="VF517_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052369.1"
                     /translation="MSRLIRMDSTGHSTLAEWTAQDEAAYRSASEAFRRELDNGYIGM
                     VSEGPGHATHVRELPVDASLVIMRQPIAGG"
     gene            3580..4233
                     /locus_tag="VF517_05210"
     CDS             3580..4233
                     /locus_tag="VF517_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052370.1"
                     /translation="MAFAESVPGAAGQPLPELASVPWRRRASAAGLRTRGRAWTWFTA
                     VYVAQWFAVAAILLALEPLTAPVAAAGLAMAYIVPSLYAARGATTLRPKGARHASAEP
                     VAQGLLGDLLGHDARDLHRHTGLAVERGRLGTWVIGEGGAALVTDGGRRVHCFCVRTT
                     DPQLPPSDRIAHLLLALRTDEEGFATVANHAFAGAPWRLARHIDRRMRPALTAARRS"
     gene            4236..5522
                     /locus_tag="VF517_05215"
     CDS             4236..5522
                     /locus_tag="VF517_05215"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8052371.1"
                     /translation="MAWRVVIAGGGFGGLYAARRLERVLPSQAAHVTLVTDVNFLLYT
                     PLLPGAAAGTLEPRHVVIPVREELERTEIRLGRVTSLDPGGSSICFRSADGRDERMEY
                     DQLVLAVGSISRVLPIPGLAEHGMGFKTLPEAVALRNHALLNLEIAEQLDDPELRRPY
                     LSFVFVGAGYAGVEGIAELQDYVGDVIERYPRCRLDGTRWVLVEAQERIMPEISPSLA
                     AFATSELRARGIEVRANTRIERVEAGSVTLTGGEAVPARTLCWTAGVTPPPLVAESGL
                     PLGAGGRVAVDATMRVDGHPNIWAIGDAAAVPDPAKGGKGPTPPTCQHALRQGKVVAD
                     NVAGALGHGRIRKFRYRTLGVFVDMGRKQAVAEVLKLRLRGFPAWWLARTYHLWMMPG
                     AARKARLVTDWTVGLFFSRGSAELGQLGHPPPLDPP"
     gene            5626..7392
                     /locus_tag="VF517_05220"
     CDS             5626..7392
                     /locus_tag="VF517_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8052372.1"
                     /translation="MGRTRTAAALALTALGIAAAPAPAADFWREPDGGPSPINVSGDV
                     SPGADSGSDLVDLGGVPYIAWSEWNGTAYQVRVARLEGSQWVVLGPTLNANPTRDATQ
                     PHLAVNEGVLWATWLERDATGTTQIRVARRTGTAWVPVGGAVDVYPNQPDDPRFYAGA
                     SQLAFLGGVAYLGVMEDSGVDFSFDLLKLNANEQWVKADTGTRPFHTTPRSFELEVSG
                     GRLYLATTEFFGGSVVWRLAGNGRDWENIGPPNSDQLSPTDIEDVGGTLRATYDGKLW
                     TLTAGGTWQLVGPAPGGLGTGFETPTVLTKTQAGDLAVARLVANAWQPLGSGLLDNRE
                     KYVGAGLVEVGNTPYVSWSEFDGCNWELRVARLESATADDPPLPAPGACPPPKQPPSN
                     DPGTGPNPGPTPNPNPNPGPNPNPGPNPPAPTPPVLGDCGILLSGTPGADALNGDARR
                     NEISGLAGDDRIFGAGNADCLFGGAGNDLLDGGAGNDELRGGSGHDRLKGGADSDELF
                     GDSGNDRLTGGDDEDELTGGSGSDVLGGGDGYDTVSAGSGNDVIDARGSGFDRIDCGP
                     GRDRVKNADRLDRLRGCERVSR"
     gene            7438..8385
                     /locus_tag="VF517_05225"
     CDS             7438..8385
                     /locus_tag="VF517_05225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8052373.1"
                     /translation="MIAEMTFREGSLADLPATFELSEVAMHESAVRQGVIAPDPPLTG
                     NRIRADWQRQRGVLEFLAAQPDGRYVICEGPEGVTGFARVVRFGAMEQLTELMVHPGH
                     QRQGIGRALLERCWPGDPTPELGRIVVAAGAPADLTLYTEFGTMPVAGHWHMRARTED
                     YVPLRTAEVDMVDPGVHVLTPDRAVEEWCRLEPEAVGHERPALHAYFARERTCLALPG
                     QDERPSALCWVSSDGEIGPAVAAAAGDLVPVILAALDRVAKTQEPELLSVFTTSLSWW
                     LLRRLRTLGFQVFWPSWVMCSVPLPGLDRYVPTRPPQVI"
     gene            8416..8589
                     /locus_tag="VF517_05230"
     CDS             8416..8589
                     /locus_tag="VF517_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052374.1"
                     /translation="MNERLSNIGGKLLAGVVLLIAGWILLKFVLGFIAWVATVVMVLI
                     AVVAVLWAVNRLL"
     gene            8597..8872
                     /locus_tag="VF517_05235"
     CDS             8597..8872
                     /locus_tag="VF517_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052375.1"
                     /translation="MELVPIMVFCGLSAAIIGKIKGSSFIVWFLVGAALPIAGTIAAL
                     LYRWERDELRRECEECGNIVPLSDQVCMRCGRDLDWPEQAFRTTPAR"
     gene            8933..10609
                     /gene="ilvB"
                     /locus_tag="VF517_05240"
     CDS             8933..10609
                     /gene="ilvB"
                     /locus_tag="VF517_05240"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX8052376.1"
                     /translation="MRAVDAIIECLKAEGVDTVFGIPGGANLPTYDALYDGGVRHVLC
                     RHEQGAGHAAEGYAKASGKVGVALATSGPGATNLVTAIADAVMDSVPTVFITGQVRTE
                     LIGTDGFQEADISGITMPIVKHSLMIQDPREIPAAIHEAFHVARSGRPGPVLIDIPQD
                     LSRADIPYEPVDSTSLPGYNPTTEGNARQIKLAAKALANARRPVFYTGGGVVNANAAE
                     ELRELCFADRFPVTSTVMGLGAFPAPHEQWLGMLGMHGTRTANYAMDEADLIVAIGAR
                     FDDRITGKLSEFAPRAKFIHIDIDPAEISKNVPAHIPIVGDAKRILPRLTREYRALET
                     DSSRLDGWWERIKGWQERHPLRYDDSEDSEIKPQFMIQALHEATGGDAIVTSDVGQHQ
                     MWTAQWYDFPQPRRWINSGGLGTMGFGLPSALGAKVACPDQTVVCVAGDGSMQMNSQE
                     LATCVSEGIAVKTFIMNNGYLGMVRQWQELFWDRRYSAVDMGTTPDWVKLAEAYGAVG
                     MRATDKTTLVEQMREALAADGPVVMDVVVTKEENCYPMIPAGQPAREMVG"
     gene            10706..11263
                     /gene="ilvN"
                     /locus_tag="VF517_05245"
     CDS             10706..11263
                     /gene="ilvN"
                     /locus_tag="VF517_05245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX8052377.1"
                     /translation="MGEPGTREILNLGDLEAAGGIHTGRKHILSLLVENKPGVLARIA
                     GLFSRRGFNIQTLAVGPTDDPTLSRVTLTVDGALHPIDQVTKQLHKLVNVIKIRDLEP
                     HEAVARELALFKVSADGESRGQIMQFADIFRGKVVDVSKRSVTIEITGTDDKIEAFER
                     MVRPFGLIEMVRTGEIAISRGRSTT"
     gene            11268..12695
                     /locus_tag="VF517_05250"
     CDS             11268..12695
                     /locus_tag="VF517_05250"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX8052378.1"
                     /translation="MTVIGGLRTEHGPLGLSEAARQLLRARLDRAIKLARRQRRPILA
                     SVRVPADAAIDASATVLAARTPSDSAFCFEQPDRDRFAIAALGSAAVVEASGERRFES
                     VAEQCRDLTCGAIADGADPVWVGGFAFAPDGAATPEWSSFGPARLTLPEVAITRRGDR
                     AELTVNAVVTGDEAPDAVLERCADRTETLVPVHMPLVDPHPVERARVVSAAPPEHFAE
                     AVRRATERIAAGELEKIVLAREVRVHAPRPIDAAAVYGGLRDRFPSCYCYLAVTPDAA
                     FVGATPELLVRRDGQRAQTVALAGTLRRSADPALDRHLAEQLLGSERYREEQAIVTRR
                     IERTLGPVSVWVAAAEEPVVVKVQNVQHLGTPIRAQLAEPLACAELAGRLHPTPAVGG
                     EPEERALPLIPALEGLDRGWYAAPLGWTDLAEDGEFCVALRCALIRDRVAHLYAGEGI
                     VRESVPDEELAANEAKLQALLPLLT"
     gene            complement(12692..14347)
                     /gene="menD"
                     /locus_tag="VF517_05255"
     CDS             complement(12692..14347)
                     /gene="menD"
                     /locus_tag="VF517_05255"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX8052379.1"
                     /translation="MIPINRTYAPLQAFVDELVRCGMTDAVTSPGARNAPIVLSIAAQ
                     PGVRAHSVVDERAAGFVALGLAKRSRRPVAITCTSGSAAANLMPAVVEAREAGVPLIV
                     LTADRPPELREVGAGQSIDQVKLYGSHAKWFVEVGNHEPSRTTAIHHRALACRAYWTA
                     LDGRPAPVHLNFPLRDPLAPVEEELDAETWEGRPDGAPWVRRGPATPPSPTPLEPAAR
                     GAIVAGAMPPGSPLPALAARLGWPLLADATSNLRCGPHERSAVIAHYDVLLRAERFCT
                     EHRPDFVLRAGDLPTSKPLRTWLAGVPQWVMDPQRTWQDPAHEADLWLDAVEAAPGAE
                     PADPAWLESWRRADALVPAALAEVADPFEPRVWTAAAEAAPAGSLLWVASSMPIRHVE
                     TFVPGGEKPLLFLANRGANGIDGTVASAAGAALAHDGRTIVLLGDLALLHDLGGVVAA
                     QRLGAELTIVFANNGGGNIFDHLPLAAVADPATFERHIVTPSEVDGEHVAALAGMDYV
                     RAETPDAVRAAVEGGPALVEAPTDRARNVALQRELFERVAARL"
     gene            14354..15082
                     /locus_tag="VF517_05260"
     CDS             14354..15082
                     /locus_tag="VF517_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8052380.1"
                     /translation="MQAPLVVFVPGFMQRGEAWAPVAGIVSESYTTLCVDFAANTLAG
                     RLDELRAAAPPGAVAVGYSMGGRLALQLAAREPERFGALVTVGAAAGIEDPGERRARR
                     EADEALATWIEGQPIEAVVERWESQPVFAGQSPELVEAQRAGRRSHRPGELAELLRSA
                     GQGAAEPVWERIGSLPMPLLAIAGERDERYAAAARRLAELAPRGRCELVPGAGHAAHL
                     ERPDAVAELILGALASWADSGSAS"
     gene            complement(15098..>15442)
                     /locus_tag="VF517_05265"
     CDS             complement(15098..>15442)
                     /locus_tag="VF517_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX8052381.1"
                     /translation="EAEACDAVNVKLAPSGGFDAARATIAAAREHGLEPFLSSTLDGP
                     WGIAAALQLAATERLSLACGLATLDLFDAAVARALPAPRTGLLAVPEGPGLGVAVDDD
                     ALAEVLVEALPV"
BASE COUNT         2081 a         5725 c         5664 g         1972 t
ORIGIN      
        1 aatgcggaag ccctccgccg cgccgcgcct ggcagtggcc gctcgcgatc gggctgctcg
       61 gcctcatggc cctcctcgcg cgcaggtggg ctacctacaa tcgctcgccg tgatcgagcg
      121 cgagcaggtc ctccacgtcg cccgccttgc acgcctcgag ctgagcgacg aggaggtcga
      181 gcgcatgtcc ggcgagctgt cgtcgatcct cgaccacatc gagaagatca acgagctcgc
      241 cctcgacgac gtggcgccca cctcgcacgt gatcggagtg gagaacgtcc tgcgtcccga
      301 cgagccgcgc cccagcatcc cgcgcgagcg cgccctcgag ctcgcgccgg acgccaccga
      361 cgacgggttc cgcgtgccgc cgagccgggc gccggacgcg gcgtgagcgg cctcctcgac
      421 ctgaccggcg cgcaggccgc cgagcgcatc cgcgccggcg agacctcagc gaaggagctc
      481 ttcgacgcct accgcgagcg cgcggccgcc gaggacctca acgcgttcct ctggctggcc
      541 gacgcggcgc cggatgccga gcccgcgcag gacgctccgc tcggcggcgt cccgctcgcc
      601 gtcaaggacc tgttctgcgt cgagggcgtg ccgagccagg ccgcctcacg catcctcgag
      661 ggctaccgcc cgccgtacac cgccaccgcc gtcgagcggc tcaccgccgc cggcgcgccc
      721 gtgctcggca agaccaacat ggacgagttc gcgatgggct cctcgaacga gaactcgggc
      781 tacggcccgg tgctcaaccc ttgggaccgc gagcgcgtgc cgggcggctc ctcgggcgga
      841 agcgccgccg ccgtcgccgc cggcctcgct ccgtgggcga tcggcaccga caccggcggc
      901 tcgatccgcc agcccgccgc cctctgcggc gtggtcggcc tcaagccgac ctacggtgcg
      961 gtctcgcgtt acgggatgat cgccttcgcc tcgtcgctcg accagtgcgg cccgatcacg
     1021 cgcgacgtga ccgattgcgc gctgctgctc aagcacatgg tcggccgcga cccgtgcgac
     1081 tccacctcgc tcgacttccc cgagccggtc gagctcccga ccctgatccg cctcgacggc
     1141 atgcgcttcg gcgtgccgcc tgagctgagc ggcgagggga tcgagcccgg cgtccggcag
     1201 gtcttcgagg acacgctgaa gctgatcgag gagctcggcg ggacgatcga ggaggtcgcc
     1261 ctgccgcact cgccgcaggc aatctccgcc tactacgtga tcgcccccgc ggaggcgagc
     1321 gcgaacctcg cgcgctacga cggcgtgcgc tacggcaccc gccatggcga gggcgacctc
     1381 acgtcgctct acgaggagac ccgcgccacg ggcttcggcg acgaggtcaa gcgccggatc
     1441 atgctcggca cctacgcgct ctcgtcgggt tattacgagg cctactacgg ccgcgctcag
     1501 cgcgtgcgca cgaagatcgc cgacgacttc cgcaacgcgt tcgagaaggt cgacctgatc
     1561 gtcacgccga cctcgccgac cgtcgccttc aggctcggcg agcgcaccga cgacccgctc
     1621 gccatgtacc tgtccgacta ctgcacggtc tcgatgccgc tggccggcat ccccgcgatc
     1681 tcgatccccg gcggcctggc cggcggcctg ccggtcggca tccagctcgc cggcccggcg
     1741 ttcagcgaga accgcatcct cgacgccgcg cacgcgatcg agcaggcggt cggcttcgac
     1801 gggagctccg cccgtggctg aggcggccct cgccgagcgc cactacgagc cggtcatcgg
     1861 gctcgagatc cacgtccagc tcaggacgcg tacgaagatg ttctgcggct gcgcgctgtc
     1921 gttcggggag gagcccaaca cgcgcacctg cccgatctgc ctcggccacc ccggcacgct
     1981 cccggtgacc aacgccgagg ccgtgcacta cgggatcatg atcgcgctcg cgctcgggtg
     2041 cgaggtggcc ccgcggtcga tcttccaccg caagaactac ttctatcccg acctcccgaa
     2101 gggctaccag gtctcccagt acgacatccc gctggggagc gccgggcgcc tcggcgaggt
     2161 gcgcgtgcac cgcgtgcacc tggaggagga cgccgcgaag ctcgtgcacg cgggcagctc
     2221 gggccgcatc cacggcgccg aggcgagcgt ggtcgacttc aatcgcggcg gcacgccgct
     2281 cgtcgagatc gtgtccgagc cggacctgag gagcgccgcc gacgccggcg agtgggcgcg
     2341 cctgctgcgc gccacgctga gggcgctcgg ggtctccgac gtgaacatgg aggagggctc
     2401 gctgcgcgtg gacgcgaacg tgtcgatccg cccggccggc gagtcgacgc tcggcaccaa
     2461 gaccgagctg aagaacatga actcgttccg cttcctggag cgcgggatcg aggccgagat
     2521 cgcccgccag gaggcgatcc tgcgcgacgg cggcgaggtc caccaggaga cgctccacta
     2581 cgacccgcag agcggcgcga tcagctcgct gcggtcgaag gaggaggcac acgactaccg
     2641 ctacttcccg gagcccgacc tcgtgccgat cgctccgacc gaggcgatga tgacccgcgc
     2701 ccgcgaggcg ctgcccgagc tgccggccgc gcgtgcggag cgcttcgagt ccgagctcgg
     2761 actgcccgcg gccaccgcca agctgctggc gttccgcggc gagctgggcg acttctacga
     2821 ggagacggtc gccgcggcgg acggcggggc cgagccggcg gcgatcgcca actgggtcgt
     2881 gggcgagctg ctcgcgcggc tgggggatgt cgagcccggg gactcccgcg tggagcccgc
     2941 cgcgctggcc aagctcgtgg cgatggtgtc cgagaagcgg atctccgggg ccgccggcaa
     3001 ggaggtgctc ggagtgctgg ccgacgcggg cggcgacccc gaggcgatcg ccgccgagcg
     3061 ctcgctcggc atggccgaca cgagcgagct cgacgcgatc gtcgaccgtg cgatcgagca
     3121 gaacgcggac gccgtcgaga agatccgcgg cggtgccgac aaggcgatcg gcgcgatcgt
     3181 cggcgcggtg atgcgcgaga ccaagggccg cgccgacggc ggcgaggtcc agcggatgat
     3241 ccgcgagcgg atcggctgag cgccgcgctc gccgatcggc cgagagtcac acaagccggc
     3301 cgaacgcgcg gcgtatcatc gaccgcctca ccccacagga ggcctcacat gtcgcgactg
     3361 atccgtatgg actcgaccgg ccactcgaca ctggcggagt ggaccgcgca ggacgaggcg
     3421 gcgtaccgca gcgcctccga ggccttccgc cgcgagctcg acaacggcta catcggcatg
     3481 gtgagcgagg gccccggcca cgccacgcac gtgcgcgagc tgcccgtgga cgcgagcctc
     3541 gtcatcatgc gccagcccat cgcgggcggc tagcgagggg tggcattcgc cgagtcggtt
     3601 ccgggggcgg ctggccaacc ccttcccgag ctcgcctccg tcccctggcg gcggcgggcc
     3661 agcgccgccg gcctgcgcac gcgcgggcgg gcgtggacgt ggttcacggc cgtctacgtg
     3721 gcccagtggt tcgccgtggc ggcgatcctg ctcgcgctcg agccgctgac cgccccggtc
     3781 gcggcggccg gcctggcgat ggcctacatc gtgccgagcc tctacgcggc ccgcggcgcc
     3841 accaccctgc gcccgaaggg cgcccgccac gccagcgccg agcccgtcgc gcagggtctc
     3901 ctcggcgacc tgctcggcca cgacgcgcgc gacctgcacc ggcacaccgg cctggcggtc
     3961 gagcgcggcc ggctcggcac ctgggtgatc ggcgagggcg gcgcggcgct cgtgaccgac
     4021 ggcggccgcc gggtgcactg cttctgcgtg cgcaccaccg acccgcagct cccgccctcg
     4081 gaccggatcg cgcacctgct gctcgccctg cgcaccgacg aggagggctt cgcgaccgtc
     4141 gccaaccacg ccttcgccgg cgccccttgg cggctcgcgc gccacatcga ccggcggatg
     4201 cgcccggcgc tgaccgcggc gcggcggagc tgaccttggc ctggcgggtc gtgatcgccg
     4261 gcggcgggtt cggcgggctc tacgcggccc ggaggctcga gcgcgtgctg ccctcgcagg
     4321 cggcccacgt cacgctcgtt accgacgtca acttcctgct ctacaccccg ctcctgcccg
     4381 gcgcggccgc cggcacgctc gagccgcgcc acgtcgtgat cccggtccgc gaggagctcg
     4441 agcgcaccga gatccggctg gggcgcgtga cctcgctcga ccccggcggc tcgagcatct
     4501 gcttccggtc ggcggacggg cgcgacgagc gcatggagta cgaccagctc gtgctcgccg
     4561 tcggctcgat ctcgcgcgtg ctcccgatcc ccgggctcgc ggagcacggc atgggcttca
     4621 agacgctccc cgaggccgtg gcgctgcgca accacgcgct gctcaacctc gagatcgccg
     4681 agcagctcga cgaccccgag ctgcgccgcc cctacctgtc gttcgtgttc gtcggagccg
     4741 gctacgccgg ggtggagggc atcgccgagc tccaggacta cgtcggcgac gtgatcgagc
     4801 gctacccgcg ctgccgcctc gacggcacgc gctgggtcct cgtcgaggcg caggagcgga
     4861 tcatgcccga gatctcgccc agcctggcgg cgttcgcgac gtccgagctg cgcgcccgcg
     4921 gcatcgaggt ccgcgcgaac acgcggatcg agcgggtcga ggccggcagc gtcacgctca
     4981 ccggcggcga ggccgtgccc gcgcgcacgc tctgctggac ggccggagtg acgccgccgc
     5041 cgctcgtggc cgagtcgggg ctgccgctcg gtgcgggcgg tcgcgtggcc gtggacgcca
     5101 ccatgcgcgt ggacggccat cccaacatct gggcgatcgg ggacgccgcc gcggtgcccg
     5161 accccgcgaa gggtgggaag ggccccaccc cgcccacctg ccagcacgcc ctgcgccagg
     5221 gcaaggtcgt ggccgacaac gtggcgggcg cgctcgggca cgggcggatc cggaagttcc
     5281 gctaccggac gctcggcgtg ttcgtcgaca tggggcgcaa gcaggccgtg gccgaggtgc
     5341 tgaagctgag gctgcgcggc ttcccggcct ggtggctggc gcgcacctac cacctctgga
     5401 tgatgccggg cgccgcgcgc aaggcccggc tcgtgaccga ctggaccgtc ggcctcttct
     5461 tcagccgcgg ctcggccgag ctcgggcagc tcggccaccc gccgccgctc gacccaccct
     5521 gaacatccgg gcttaacttt tcggggctgg cgcgtcagcc cgcatggggt tagggtggac
     5581 gcgtcgaagg atcttgagcg ccccgggggg catggaggca gcagcatggg acggactcgc
     5641 acggcagcag cgctcgcact gacggcgctc ggcatcgcgg cggcaccggc tccggccgcc
     5701 gacttttggc gcgagcccga cggcgggccc agcccgatca acgtcagcgg cgacgtctcg
     5761 cccggtgccg acagcggctc cgacctcgtc gacctcggcg gcgtgccgta catcgcctgg
     5821 agcgagtgga acggcaccgc gtaccaggtg cgcgtcgcgc ggctcgaggg cagccagtgg
     5881 gtggtgctcg ggccgacgct caacgcgaac cccacgcgcg atgccaccca gccgcacctc
     5941 gcggtcaacg agggcgtgct gtgggccacc tggctcgagc gcgacgcgac gggcaccacc
     6001 cagatccggg tcgcgcgccg gaccggaacc gcgtgggtcc cggtcggcgg agcggtggac
     6061 gtctacccga accagcccga cgatccccgc ttctacgcgg gcgcctcgca gctcgcgttc
     6121 ctcggcggcg tggcctatct cggcgtgatg gaggactccg gcgtcgactt ctcgttcgac
     6181 ctcctgaagc tgaacgcgaa cgagcagtgg gtgaaggcgg acaccggcac ccgcccgttc
     6241 cacaccaccc cgcgatcgtt cgagctcgag gtctccggcg ggcgcctgta cctcgcgacg
     6301 acggagttct tcggcggctc ggtggtctgg cgcctcgcgg gcaacggccg cgactgggag
     6361 aacatcggtc cgcccaactc agaccagctg agcccgaccg atatcgagga cgtcgggggc
     6421 acgcttcgcg cgacctacga cggcaagctc tggaccctca cagcgggcgg cacctggcag
     6481 ctcgtcggcc cggcgcccgg cggtctcggc acggggttcg agaccccgac cgtgctcacg
     6541 aagacgcagg ccggcgacct cgccgtggca cgcctcgtcg cgaacgcctg gcagccgctc
     6601 ggcagcggcc tgctcgacaa ccgggagaag tacgttggcg ccgggctggt cgaggtcggc
     6661 aacacgccgt acgtctcctg gtccgagttc gacggctgca actgggagct gcgcgtggcg
     6721 cggctcgagt cggcgaccgc cgacgacccg ccgctgccgg ccccgggcgc gtgcccgcca
     6781 cccaagcagc cgccgtcgaa cgatcccggc acgggtccga acccggggcc gacccccaac
     6841 ccgaacccga acccgggacc gaaccccaac ccgggtccca acccgcccgc gcccacgccg
     6901 ccggtcctcg gcgactgcgg gatcctgctc agcggcaccc cgggggccga cgcgctgaac
     6961 ggcgacgccc gccgcaacga gatcagcggg ctcgcgggcg acgaccgcat cttcggcgcc
     7021 ggtaacgccg actgcctgtt cggcggggcc ggcaacgacc tgctcgacgg cggcgccggc
     7081 aacgacgagc tgcgcggcgg atcgggccac gaccgcctga agggcggcgc ggactccgac
     7141 gagctgttcg gcgactccgg caacgaccgg ctcaccggcg gcgacgacga ggacgagctc
     7201 accggcggct ccgggagcga cgtgctgggc ggcggcgacg gctacgacac cgtgagcgcc
     7261 ggctccggca acgacgtgat cgacgcccgc ggctcgggct tcgaccgcat cgactgcggc
     7321 cccgggcgcg accgcgtgaa gaacgccgac cgcctggacc ggctccgcgg ctgcgagcgc
     7381 gtgtcgcgct gaggccggtc ccgggcggcg tctgacccgg ctgcctagac tcgccgggtg
     7441 atcgcggaga tgaccttccg ggaggggtcg ctcgccgacc tcccggcgac cttcgagctc
     7501 tccgaggtcg cgatgcacga gtccgcggtg cgccagggcg tgatcgcgcc cgacccgccg
     7561 ctgaccggca atcgcatacg cgccgactgg cagcgccagc gcggcgtgct cgagttcctg
     7621 gccgctcagc cggacggccg ctacgtgatc tgcgagggcc ccgagggggt gaccggcttt
     7681 gcccgcgtgg tgcgcttcgg agccatggag cagctcaccg agctgatggt ccacccgggc
     7741 caccagcgcc agggcatcgg gcgggcgctg ctcgagcgct gctggcctgg cgacccgacg
     7801 ccggagctcg gccggatcgt ggtggcggcg ggcgcgcccg ccgacctcac gctctacacg
     7861 gagttcggca cgatgcccgt ggccggccac tggcacatgc gcgcccgcac cgaggactac
     7921 gtgccgctgc gcacggcgga ggtggacatg gtcgacccgg gggtgcacgt gctcacgccc
     7981 gaccgcgccg tggaggagtg gtgccgtctc gagcccgagg ccgtcgggca cgagcgtccc
     8041 gctctgcatg cctacttcgc gcgcgagcgc acctgcctcg cgctgcccgg gcaggacgag
     8101 cggccgtccg cgctctgctg ggtcagctcg gacggtgaga tcggccccgc ggtcgccgcc
     8161 gccgccggcg acctcgtgcc cgtgatcctc gcggcgctcg accgcgtggc gaagacgcag
     8221 gagcccgagc tcctgagcgt gttcacgacc tccctgagct ggtggctgct gcggcgcctg
     8281 cgcacgctcg gcttccaggt cttctggccg agctgggtga tgtgctcggt cccgctgccg
     8341 gggctcgacc gctacgtgcc cacgcgcccg ccgcaggtca tctgacgctt agatgcgtct
     8401 gcgagaatcg gcgtcatgaa cgaacgcctc tccaacatcg gcggcaagct gctggccggc
     8461 gtggtcctgc tgatcgccgg ctggatcctc ctgaagttcg tgctcgggtt catcgcctgg
     8521 gtggcgacgg tggtcatggt gctgatcgcc gtggtcgcgg tcctgtgggc ggtcaaccgc
     8581 ctactctgag gcggccgtgg agctcgtccc gatcatggtg ttctgcggcc tctcggccgc
     8641 gatcatcggc aagatcaagg gcagctcgtt catcgtctgg ttcctggtcg gcgcggccct
     8701 cccgatcgcg ggcacgatcg ccgcgctgct ctaccgctgg gagcgcgacg agctgcgccg
     8761 cgagtgcgag gaatgcggga acatcgtgcc gctgagcgac caggtctgca tgcgctgcgg
     8821 gcgcgatctc gactggcccg agcaggcctt ccggaccacg cccgcgcgct agactcggcc
     8881 tcccgcgcgg cctcccggtc gccagccccg accccgcaag gagtggtccc gagtgcgagc
     8941 cgtcgacgcc atcatcgagt gcctcaaggc cgagggcgtc gacaccgtat tcggcatccc
     9001 cggcggcgcc aacctgccga cctacgacgc cctctacgac ggcggcgtcc gccacgtgct
     9061 ctgccgccac gagcagggcg ccggccacgc ggccgagggg tacgcgaagg cctccggcaa
     9121 ggtgggcgtg gccctggcca cctcgggccc gggcgccacg aacctcgtca ccgcgatcgc
     9181 cgacgcggtc atggactcgg tcccgaccgt gttcatcacc ggccaggtcc gcaccgagct
     9241 gatcggcacc gacggcttcc aggaggccga catctccggc atcaccatgc cgatcgtcaa
     9301 gcactcgctg atgatccagg acccgcgcga gatccccgcg gcgatccacg aggccttcca
     9361 cgtggcccgc tcggggcgcc ccggcccggt gctcatcgac atcccgcagg acctctcgcg
     9421 cgcggacatc ccgtacgagc cggtcgactc gacctcgctg ccgggctaca acccgaccac
     9481 cgagggcaac gcgcggcaga tcaagctggc cgcgaaggcg ctcgccaacg cccggcggcc
     9541 ggtcttctac accggcgggg gcgtggtcaa cgccaacgcg gcggaggagc tgcgcgagct
     9601 ctgcttcgcg gaccgcttcc cggtcacctc gaccgtgatg ggcctcgggg cgttcccggc
     9661 cccgcacgag cagtggctcg ggatgctcgg catgcacggc acccgcaccg cgaactacgc
     9721 gatggacgag gccgacctga tcgtcgcgat cggcgcccgc ttcgacgacc ggatcaccgg
     9781 caagctgtcg gagttcgcgc cgcgcgcgaa gttcatccac atcgacatcg acccggccga
     9841 gatctccaag aacgtgccgg cccacatccc gatcgtgggc gacgcgaagc ggatcctgcc
     9901 gcggctcacc cgcgagtacc gggcgctcga gaccgactcg tcgcggctcg acggctggtg
     9961 ggagcggatc aagggctggc aggagcgcca cccgctgcgc tacgacgact ccgaggacag
    10021 cgagatcaag ccgcagttca tgatccaggc gctgcacgag gccacgggcg gcgacgcgat
    10081 cgtcacctcg gacgtcgggc agcatcagat gtggaccgcg cagtggtacg acttccccca
    10141 gccgcgccgc tggatcaact cgggcgggct cgggacgatg ggcttcgggc tgccctcggc
    10201 gctcggcgcc aaggtcgcct gccccgacca gacggtcgtg tgcgtcgcgg gcgacggctc
    10261 gatgcagatg aactcgcagg agctcgccac ctgcgtgtcc gagggcatcg cggtgaagac
    10321 gttcatcatg aacaacggct acctcggcat ggtccgccag tggcaggagc tgttctggga
    10381 ccgccgctac tcggccgtgg acatgggcac gacgcccgac tgggtcaagc tggccgaggc
    10441 ctacggcgcg gtcggcatgc gcgcgaccga caagaccacg ctcgtcgagc agatgcgcga
    10501 ggcgctggcc gccgacggcc cggtcgtcat ggacgtcgtc gtgaccaagg aggagaactg
    10561 ctatccgatg atccccgcgg gccagccggc acgagagatg gtgggatgac gagcgaaaac
    10621 caagccagcc aaggacgcag gcccgaaggc gtgggcgacc aagccgaggg ccgagggcgc
    10681 cggttggcgc aggtttgcag ccgtcatggg tgagccaggg acaagggaga ttctgaacct
    10741 cggcgacctc gaggccgccg gggggatcca caccggccgc aagcacatcc tgtcgctctt
    10801 ggtcgagaac aagccgggcg tgctggcgcg gatcgcgggg ctgttctcgc ggcgcgggtt
    10861 caacatccag acgctcgcgg tgggcccgac cgacgacccg accctgtcgc gcgtgacgct
    10921 gacggtcgac ggcgcgctgc acccgatcga ccaggtgacc aagcagctcc acaagctggt
    10981 caacgtgatc aagatccgcg acctcgagcc ccacgaggcc gtggcccgcg agctcgcgct
    11041 gttcaaggtc agcgccgacg gcgagtcgcg cgggcagatc atgcagttcg ccgacatctt
    11101 ccgcggcaag gtggtcgacg tctcgaagcg ctcggtgacc atcgagatca ccggcaccga
    11161 cgacaagatc gaggcgttcg agcggatggt caggccgttc gggctgatcg agatggtccg
    11221 caccggcgag atcgcgatct cgcgcggacg ctccaccacc tagcgccgtg acggtcatcg
    11281 gtggccttcg caccgagcac ggcccgctgg ggctctcgga ggcggcgcgg cagctgcttc
    11341 gtgcacggct cgaccgcgcc atcaagctgg ctcggcgcca gcggcgcccg atcctcgcga
    11401 gcgtgagggt cccggccgac gccgcgatcg acgcgtccgc cacggtgctg gccgcgcgca
    11461 cgccgagcga ctcggcgttc tgcttcgagc agcccgaccg cgaccgcttc gccatcgccg
    11521 cgcttggctc cgcggcggtc gtcgaggcga gcggtgaaag gcgcttcgag tccgttgccg
    11581 agcagtgccg cgacctgacc tgcggcgcca tcgcggacgg ggccgacccc gtgtgggttg
    11641 gcggcttcgc cttcgcgccc gacggggcgg cgacgcccga gtggtcctcg ttcggccccg
    11701 cgcggctcac gctgccggag gtcgcgatca cgcgccgcgg ggaccgcgcc gagctcacgg
    11761 tgaacgcggt cgtgaccggc gacgaggcgc cggacgccgt gctcgaacgt tgcgccgacc
    11821 gcaccgagac gctcgtgccg gtgcacatgc cgctggtgga cccgcacccg gtcgagcgcg
    11881 cgcgggtggt cagcgccgcg ccacccgagc acttcgccga ggcggtgcgg cgagcgaccg
    11941 agcggatcgc ggcgggtgag ctcgagaaga tcgtgctggc gcgcgaggtc cgcgtgcacg
    12001 cgccgcggcc gatcgacgcc gcggccgtct acggcgggct gcgcgaccgg ttcccctcgt
    12061 gctactgcta cctcgccgtg acgcccgacg ccgcgttcgt cggtgcgacc ccggagctgc
    12121 tcgtccgccg cgacggtcag cgggcgcaga ccgtcgcgct cgccggcacg ctgcgccgca
    12181 gcgccgaccc ggcgctcgac cgccacctcg cggagcagct cctcggcagc gagcgctacc
    12241 gcgaggagca ggcgatcgtg accaggcgga tcgagcgcac gctaggcccc gtgagcgtgt
    12301 gggtggccgc cgccgaggag ccggtcgtgg tcaaggtcca gaacgtccag cacctgggca
    12361 cgccgattcg cgcgcagctc gccgagccgc tggcctgcgc ggagctggcc ggtcggctgc
    12421 acccgacgcc cgccgtcggc ggcgagccgg aggagcgcgc gctgccgctg atcccggcgc
    12481 tcgaggggct cgaccgcggc tggtacgcgg ccccgctcgg gtggaccgac ctggccgagg
    12541 acggcgagtt ctgcgtggcc ctgcgctgcg cgctgatccg cgaccgcgtc gcccacctgt
    12601 acgcgggcga gggcatcgtc cgcgagtcgg tgccggacga ggagctcgcc gccaacgagg
    12661 cgaagctcca ggcgctgctg ccgctcctga cctagaggcg cgccgcgacg cgctcgaaca
    12721 gctcgcgctg gagcgcgacg ttgcgggcgc ggtccgtggg cgcctcgacc agtgccggcc
    12781 cgccctcgac ggcggcgcgc accgcgtccg gggtctccgc gcgcacgtag tccatcccgg
    12841 cgagcgcggc cacgtgctcg ccgtcgacct cgctcggcgt gacgatgtgg cgctcgaagg
    12901 ttgccgggtc ggccaccgcg gcgagcggca ggtggtcgaa gatgttgccg ccgccgttgt
    12961 ttgcgaagac gatggtcagc tccgcgccca ggcgctgcgc ggccaccacg ccgccgaggt
    13021 cgtgcagcag cgccaggtcg cccagcagga cgatggtgcg cccgtcgtgc gcgagcgcgg
    13081 cgcccgcggc ggaggcgacc gtgccgtcga tcccgttcgc gccgcggttg gccaggaaca
    13141 gcagcggctt ctcgccgccc ggcacgaacg tctcgacgtg gcggatcggc atcgacgacg
    13201 cgacccagag gagcgaaccg gcgggcgcgg cctccgccgc ggcggtccag accctcggct
    13261 cgaacgggtc ggcgacctcc gccagcgccg cggggaccag cgcgtcggcg cgccgccagg
    13321 actcgagcca ggcggggtcg gccggctcgg cgcccggcgc ggcctcgacg gcgtcgagcc
    13381 acaggtcggc ctcatgcgcg gggtcctgcc aggtgcgctg cgggtccatc acccactggg
    13441 gcacgccggc cagccaggta cgcagcggct tcgaggtggg caggtcgccg gcgcgcagca
    13501 cgaagtccgg cctgtgctcg gtacagaagc gctccgcccg caggagcacg tcgtagtggg
    13561 cgatcaccgc ggagcgctcg tgcgggccgc acctgaggtt cgaggtcgcg tcggccagca
    13621 gcggccagcc gagccgcgcg gccagggcgg ggagggggga cccgggcggc atcgctcccg
    13681 ccacgatcgc gccgcgcgcc gcgggctcga ggggggtggg ggagggcggc gtggccgggc
    13741 cgcgccgcac ccagggggcg ccgtcggggc gcccctccca cgtctcggcg tcgagctcct
    13801 cctcgaccgg cgcgagcggg tcgcgcagcg ggaagttcag gtgcacgggc gcggggcggc
    13861 cgtcgagcgc ggtccagtag gcgcggcagg ccagcgcgcg atggtggatc gcggtggtgc
    13921 gcgagggctc gtggttgccg acctccacga accacttcgc gtgcgagccg tagagcttca
    13981 cctggtcgat cgactgcccc gcgcccacct cgcgcagctc gggcggccgg tcggcggtga
    14041 gcacgatcag cggcacgccg gcctcgcgtg cctcgaccac ggcgggcatc aggttcgcgg
    14101 ccgcgctgcc cgacgtgcag gtgatcgcga ccgggcggcg gctgcgcttg gccagcccga
    14161 gcgccacgaa cccggccgcg cgctcgtcga cgaccgagtg ggcgcgcacg ccgggctgcg
    14221 ccgctatcga cagcacgatc ggcgcgttgc gcgcgcccgg ggaggtgacc gcgtcggtca
    14281 tcccgcagcg cacgagctcg tccacgaacg cctggagggg cgcatacgtc cggttgatcg
    14341 ggatcatgct cgtgtgcagg cgccgctcgt ggtcttcgtt cccggcttca tgcagcgcgg
    14401 ggaggcgtgg gccccggtcg ccggaatcgt ctccgaaagc tatacgacgc tgtgcgtcga
    14461 cttcgcggcg aacaccctcg cggggcggct cgacgagctg cgcgcggcgg cgccgccggg
    14521 cgcggtcgcg gtcggctact cgatgggcgg gcggctggcg ctccagctcg cggcgcgcga
    14581 gccggagcgg ttcggcgcgc tcgtgaccgt gggggcggcg gcggggatcg aggacccggg
    14641 cgagcggcgc gcgcggcgcg aggcggacga ggcgctcgcg acctggatcg aggggcagcc
    14701 gatcgaggcg gtggtggagc gctgggagtc gcagcccgtg ttcgcgggcc agtcgcccga
    14761 gctcgtggag gctcagcggg ccggccgtcg ctcgcaccgc cccggcgagc tggcggagct
    14821 cctgcgcagc gccggccagg gcgcggcgga gcccgtgtgg gagaggatcg gctcgctgcc
    14881 gatgccgctg ctggcgatag cgggcgagcg cgacgagcgc tacgcggcgg cggcgcggcg
    14941 gctggccgag ctggcgccgc gcgggcgctg cgagctggtc ccgggcgcgg ggcacgccgc
    15001 gcacctcgag cgtccggacg cggtcgcgga gctgatcctc ggagcgctgg cgagctgggc
    15061 cgacagcggc tcggcgagct agaccgggag tgcgcggcta gaccgggagc gcctcgacga
    15121 gcacctcggc cagcgcgtcg tcgtccaccg cgacccccag cccggggccc tcgggcacag
    15181 cgagcagccc cgtgcgcggc gcgggcagcg cccgggccac ggcggcgtcg aagaggtcga
    15241 gcgtggcgag tccgcaggcg agcgagagcc gctcggtcgc cgcgagctgg agcgccgccg
    15301 cgatccccca gggcccgtcg agcgtgctcg acaggaacgg ctcgaggccg tgctcgcggg
    15361 ccgccgcgat cgtcgcgcgc gcggcgtcga acccgccgct cggcgcgagc ttgacgttca
    15421 cggcgtcgca cgcctcggcc tc
//
LOCUS       DASVPL010000105        29213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_52375, whole genome shotgun sequence.
ACCESSION   DASVPL010000105 DASVPL010000000
VERSION     DASVPL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 29213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29213
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52375"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VF517_05270"
     CDS             complement(<1..1244)
                     /locus_tag="VF517_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX8052382.1"
                     /translation="MSRPRSGKRDLERYAGLFASRTRVMKSSAMRDLMAITARPEVIS
                     LAGGLPDTASFPTETFAAMGNRIAAESCAKALQYGPTEGLDETKDCIAEVMAAEDMKV
                     DLEDVIVTTGGQQVIDLVTKTLIDPGDVIIAEGPTYPGAVPSFYSYEAEVVQIDTDEN
                     GMQVEQLRGVLDRLEREGRRPKFVYTVPTFQNPAGVTMSIERRRMLVEIAKERQLLVL
                     EDNPYGLLRYEGSAQPTLRALDGGLYVMYLGTFSKILSPGIRLGWVVAPPPILEKINL
                     GKQGADLCTSTLSQLMVRAYFDGSIWRDYVDSLNEIYRSRRDAMFDALAEFFPPQAEW
                     TRPGGGLFIWVTLPEFIDTTDLLARALRENVAFVPGEAAYLDGRGRNSMRLNFSGSDE
                     DTIREGVRRIGEVVAEQVALYGT"
     gene            1338..1949
                     /locus_tag="VF517_05275"
     CDS             1338..1949
                     /locus_tag="VF517_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX8052383.1"
                     /translation="MSLFLDIGQGAGLAGASGVRPYLPPLVAGALARGDTGIDFDGTS
                     YEFLEQPWFLVVVLALAVAAYFADRTRGTRPDESSDPVAVGLGAIGVVLGALLFAGSL
                     ADGDHTSWPGLIGGAVAAALGYFAVAALFRRARRRLRAADRSGEGAPNLLDAYADGIS
                     LVLAGLSILAPPVGIVALVVFLVLLLRARGGGGQKYEGLRVLR"
     gene            2112..3578
                     /locus_tag="VF517_05280"
     CDS             2112..3578
                     /locus_tag="VF517_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8052384.1"
                     /translation="MASKPKKLVLAVIDALKPEMLDRAIEHGRAPALKVLVENGTFVR
                     DCVSTFPSVTPVAAATIATGVMPDEHDIPSMNWYLRGEERYVEYGSSFEASRTFGLLR
                     ALYDTVYNLNMAHLSRNAPTVFERLQEADVRTAGTTYLIYRGHKRHAALEGGLYGRLA
                     KAGGFRHAVWGPDELFYADLFDSRKTECRSTLGMPGQRDQHSACVGAHLVEHDLCDFM
                     LLSLPDNDTHSHKRGPFAQVTSIASADRALERVMHAAGGPEAFLEDHAVIVMSDHAQV
                     AVSHSVNLLAEAFAEWKVLYAEGGPSDAEIAVCPSQRSAQVYVLDQERRRELLPKLVR
                     DLKVVDGVDVIAWRDGPEAAVWTRRGELRLSPGGDLTDPRGGSWHVDGDLDALGLVVT
                     DGRVDSEEYPLALARLWSALRCAHGGDVLVSAATSYEFVDWGGVAHIGGGSHGSLHRG
                     DSHAALIMCGVDGPAAGPPDQWSIADVAPLVLEHFSVA"
     gene            3587..4069
                     /locus_tag="VF517_05285"
     CDS             3587..4069
                     /locus_tag="VF517_05285"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX8052385.1"
                     /translation="MRRDSATAEPGDLALHTRVRHGLRRPHNWMQLVKFCAVGGSGYV
                     VNLCVFALAYKLFDLHHLVAATIAFVVAVINNFWWNRHWTFGAAGAREHHAGFQAARF
                     FGVSVLAFLFAAAILESLVRFTDIRPIVAQAISIVAATPLNFVGNKMWTFGRRDAGAS
                     "
     gene            complement(5614..6225)
                     /locus_tag="VF517_05290"
     CDS             complement(5614..6225)
                     /locus_tag="VF517_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8052386.1"
                     /translation="MATTPPRIPTTPPRIPPIEPPYEPDVEESLRKWMPPGAAVEPLK
                     LFRTLMIHPELAARMRPLGAALLGHPTVEPREREIVIHRATARAGAEYEWGVHAVAFG
                     RPLGLTDEQIAATAHGSADDPAWTEPRDRLLIRLVDELYDSATVSDTLWTELVREWDP
                     PQLLELVVCAGWYRLIAQVINVAGVELEPWAERFPAPAGERGE"
     gene            6314..6592
                     /locus_tag="VF517_05295"
     CDS             6314..6592
                     /locus_tag="VF517_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX8052387.1"
                     /translation="MALLDLLGRRWALRVIWELRDGPFTFRELQERCGGVSSSVLAQR
                     LAELREAGIADEGNVLTAEGRKLLEAYEPLGGWARRWARRAGAEPARR"
     gene            complement(6649..7518)
                     /locus_tag="VF517_05300"
     CDS             complement(6649..7518)
                     /locus_tag="VF517_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8052388.1"
                     /translation="MAGLAERQHGVVAAWQLLAEGRTRGEIKARLRAGRLHPLYRGVY
                     AVGHSRVTKRGRVMAAVLAYGPGALASHRTAAWLWEVLSDNRPRTDVTVTGRSGASRA
                     GIVLHRPRQLAEGDRTRVDGIPCTSVARTLLDIAATEPPRALRRAYEESERRRVLDLR
                     DVWRVLADARGHRGAKPLRALVGDATGPVPLIRSEVEARFLDLVRAEGLPLPTMNVPF
                     GEYELDAVWLDRKVVVEIDHYATHGHRRAFEGDRVRDIELQIAGFRVGRVTDEQIAEP
                     RETGARLRRLLVV"
     gene            complement(7656..8441)
                     /locus_tag="VF517_05305"
     CDS             complement(7656..8441)
                     /locus_tag="VF517_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX8052389.1"
                     /translation="MRLLFVGDIVGKPGRRALSDLLPGLREEHEPDWVVVNGENSAGG
                     LGITERIGHELLDELAVDVVTLGNHTYHHRDVYGFLDRDDRIIRPANYPKGNPGRGFT
                     VVEKGGVRLGVANLLGTVFVDAARSPFAEADAIVAELRDQADHVLLDFHAEATSEKVA
                     MGWHMDGRVTACVGTHTHVPTADYRVLPGGTAYISDVGMTGARGGVIGVKKEQALHRF
                     RTMMPIRFETSEEDPWLMAVLIDADDGGRATGIRQLLVEAPRA"
     gene            8462..9172
                     /locus_tag="VF517_05310"
     CDS             8462..9172
                     /locus_tag="VF517_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8052390.1"
                     /translation="MQEPAAAAVLEARNLEKRFGERRALKGVSLTAVSGDLVGVIGPN
                     GAGKTTLLSLLAGNAQPDAGQIVTAPEKVGWVPQQQSLYGKLTVEENLRLFARLERCE
                     DVDETVARMLEQTALQERADDQVKTLSGGNRQRLNVAIGLLGDPALLLLDEPSTALDP
                     RQRDRLWEFVTGLSDEGTTVIFTTHNVQEAERHATQLVVLADGERLFDGSPSELHTVA
                     EGTDFESAFVAFLAARGH"
     gene            9175..10530
                     /locus_tag="VF517_05315"
     CDS             9175..10530
                     /locus_tag="VF517_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8052391.1"
                     /translation="MRWLLAKDLRILRRSPLLVALLVLYPILIAALVGFAVTSGPSKP
                     RVAVLNEISDSARTIDLGGQKVDVKKEARPLFDSIEQVKVKDREEAERRVREGDVLAA
                     LIVPSTILDTLEAATQGGSGSAHVEVLYNAEDPAKRRYVENTIKARVQEANAALAKRF
                     AEAGAGYIEVLASGGKISIASLQIDVLGLGPAAAILKDVRDALPARAPERKELDQVIQ
                     FAQLGAENLGLAETLLGAVGTPIEVDSKVVEGADTPLGAFAAAVAAAVTLMFVTLLLA
                     SGALALEREENTYRRLVRGLVSHTALLVEKAGLAALCSLAVGLVLLVGLSFSADLPWG
                     RFPLWLLALGAGALAFGALGVAMGAVAREVRAASLLAFMAALPIAVLGLIPSGAVGSG
                     LYDLLRAISAVFPFRATLDALQSALGRSGDMAAPLVHLAVLTLAYGVAARLALRRFAI
                     D"
     gene            complement(10543..12063)
                     /locus_tag="VF517_05320"
     CDS             complement(10543..12063)
                     /locus_tag="VF517_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052392.1"
                     /translation="MRLFARGAAVAVVAIASWAAPASADFRRVTPDGGAFVFSTTEAL
                     TASDTDTAADVYVRAGGVNELASVGTAGGAFAQAISTDASRVFFSTAEQLSAADTDSD
                     YDLYERSRGQTTLITTGPTDPGPLPWYELAHISPDGSRVIFGTSADLVPEDTDGERDL
                     YMRSNGVTTLLSASPAGSSGPHGHYFVGASLDGTRVFEGTDEALVSNDTDGAGDFYER
                     SGGTVKLVTTGTLDTGSAPHSAWIAGGASDDGSSFIYMTDAKFELGDTNGTLDLYQRR
                     NGKTRLLTANRLGLVQPCPIPAGHGGPEPCTIFRAAQTTDGGSVFFGAGQALASTDTN
                     STWDMYLRTGSSLEHVAQGYDGWMSPDGARLVFRSVAALVPADTDSKNDLYERAGGQF
                     KLLTPGTPVEHLDLAASSRDLTHVFFVTQEGVDPADTDNRRDVYESVGGTVRLVTSGP
                     ADDHANVSDSEHTKVYPSDDGSRVYFFSKKALVAADTDVAEDAYVWEDGVTTLVSG"
     gene            12182..13156
                     /gene="hemB"
                     /locus_tag="VF517_05325"
     CDS             12182..13156
                     /gene="hemB"
                     /locus_tag="VF517_05325"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX8052393.1"
                     /translation="MAFPATRMRRLRRTGLLRGMVRETELTAAHLVYPLFVELGTDAR
                     TPIDAMPGIDRLSISHAVEEAAAAHELGVPAVLLFGIPSHKDEQASGAYDAEGVVQLA
                     TRAIKDALPDLVVMTDVCLCEYTSHGHCGVVRADGAVDNDMSLELLAKTAISQAAAGA
                     DAVAPSDMMDGRVGALRAQLDAEGHSETPIVAYSAKFASAFYGPFREAADSAPAFGDR
                     RSYQMDPANADEAVREALLDVEEGADVVMVKPALPYLDVVRRVKEATQVPVAAYNVSG
                     EYAMVKAAAQAGWLDERATVLEALTSIRRAGADIVITYHAKDVAGWLS"
     gene            13153..14223
                     /locus_tag="VF517_05330"
     CDS             13153..14223
                     /locus_tag="VF517_05330"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052394.1"
                     /translation="MTSAVPKLRSRKDGAAVPLDDLDKRLLNLLQGSFPLEPRPFLRV
                     AELAEVAEEEVLARTQRLLDERIIRQVTPIFDTRALGYKSMLVAARIDPENPHRAAKV
                     VNAHPGVSHNYLRNHDFNLWFTIAVEPDSRLGLDGTLDALAEEAGAESVRQLPTLKLF
                     KIRMDLEMEGDTKALARAAEATEPAETEPQPYDDFDVAVIRELQGDMPVVPEPYAPAA
                     RALGIPQQQLLDHLDGMRERRLLRRVAAILFHRRAGFSANGMGVWRVPDDQILEVGKR
                     MASFRGISHCYQRPTYADWPYSVFTMAHGRSKEECDAILDAIAEDTGIEERSTLYSST
                     EFKKIRLLYFTDEFKEWEAARL"
     gene            14226..15494
                     /gene="hemL"
                     /locus_tag="VF517_05335"
     CDS             14226..15494
                     /gene="hemL"
                     /locus_tag="VF517_05335"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX8052395.1"
                     /translation="MTRSQELYERALGLIPGGVNSPVRAMRSIGRDPIFVAEASGAEL
                     IDADGNRYLDWMQSWGPLVAGHAHPAVIAAVRDAAERGTTYGAATEREVDLAAEIVRR
                     VPSAEMVRMTSSGTEASMSAIRLARAATGRDRIVKFAGAYHGHVDGLLADAGSGLATQ
                     GIPASPGVTAAQAADTVVVPWNDRDAVERALAEQPVAALLCEPYPANMGLVPPAVGFL
                     AFLRDATAASGALLVFDEVISGFRVAPGGAQGREGVLPDLTILGKVIGGGLPAAAYAG
                     PAELMERVAPAGDVYQAGTLSGNPLAVAAGLATLRLLDADAYARLDATTAELAAALEA
                     AARDAGVPVQVAWTTGLLTVFLSNEPVRDYAGAKACDHEAFAALCRGLLDRGVYPPPS
                     QYEAWFPSLAHTGEHVERTAAAAREAFAEL"
     gene            15491..15835
                     /locus_tag="VF517_05340"
     CDS             15491..15835
                     /locus_tag="VF517_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052396.1"
                     /translation="MSLAADIEAGAEAVGRGSAGTREWVLEAVREGYLLHYGEPRAFD
                     GDMDQDLRLLAGDTLYALGLARLAATGDLEAVGELADLISACAHAESEGRPTGDLWDV
                     SARRLARPNPPE"
     gene            15968..16570
                     /locus_tag="VF517_05345"
     CDS             15968..16570
                     /locus_tag="VF517_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX8052397.1"
                     /translation="MPGGFEGETVTRRTLFTGGALAAGGIMTAAFTLPALGFALGPMF
                     ERTEDPRFTDVGSVRDFNTQTYVPRVITLVDDVGEAGKTTVYVRQFDARRDKDKLEQP
                     FVAISTRCMHLGCPVRYVGASQKFICPCHGGVYEFDGSVGGGPPVRPLDRFETRVRNG
                     RVQIGPRFSLNTKLERKKVRDPSNHLDGLWQYIYPSRPTV"
     gene            16567..17439
                     /locus_tag="VF517_05350"
     CDS             16567..17439
                     /locus_tag="VF517_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX8052398.1"
                     /translation="MKLLPGPLRRKNGKNGTNGNGNGNGATRVMPGDLRNGHEERTKE
                     REKEADRKRQLDAVKETGINVVGFLDERTGGGPFMRFFLYRKVPPINWFYTLGSATMF
                     AFVSQAVTGVFLAMYYRPSSNEAYESVQHITNDVFAGELVRGMHRWGATVMMILIFMH
                     MGRVFFFGGYKYPRELNWVIGVVLLVLTLMMGLTGYLLPFDQRSFWATVVAVNLNSSG
                     PIIGPYLADFLRGGAEFGATTISRFYALHMLLIPGLIMALIGAHLYLVVKLGTTAPPW
                     LKAEKARDLAKEEI"
     gene            17443..18159
                     /locus_tag="VF517_05355"
     CDS             17443..18159
                     /locus_tag="VF517_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8052399.1"
                     /translation="MNGREREQYLREYQLLKNKKGKPFFPYAILKDSAMACIVLFVII
                     AMAIILGAELGPKADPTSTTVTPRPEWYFFFLFELLRVIKPPALVFVATIGIPTIWLV
                     LLLLLPFYDRNPERHPLKRPIATTAGILTILTMAYLTIEGALAGSPTEIEMKVEKRLE
                     PGRAVAASSGCLGCHRIGENGNTGPGPALTNVGERLPRQAIARTLVNPTAPMPSYSQL
                     RQNEPEQFNQLVEFLSSLRE"
     gene            18165..19154
                     /locus_tag="VF517_05360"
     CDS             18165..19154
                     /locus_tag="VF517_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX8052400.1"
                     /translation="MPAKAAEQLGDVLARGGPRLRALLDATELRLAGVAEGYGDALSA
                     HVAQTLTAGGKRLRPILVFITGGSATGEALVAAGAAVELLHMATLVHDDVLDRAELRR
                     GTRTVYAQGGRGAATATGDLLFSRAFALLAATGEREALRALSDASSALARGELLQRED
                     AWSPAVTPERYLERCRLKTAPLFSASCRLGAILGDPGPEAADSLARFGEGIGLAFQLF
                     DDVLDVSGPPEVTGKPRGTDLLDGTVTLPLIVARERDADLRALDLRGAAHDHASAAAL
                     CDRIAATGALEETRDQALDHVRRAKAALEDVELDDAQRELLEVIANGVADRQA"
     gene            complement(19179..19811)
                     /locus_tag="VF517_05365"
     CDS             complement(19179..19811)
                     /locus_tag="VF517_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX8052401.1"
                     /translation="MTTSSVAAPPLPGRDTLVRRAKALAWLGMAWHGIEAVVAIAAGV
                     AAGSIALVGFGIDSVVEAMAGFVLLWRFAAVRSASEGAERSAQKLIAVSFYVLAAYVA
                     VEAGRGLLGGERPDGSTVGIVLAVATLITMPPLARAKVRLAHELHSSATKAEGRQNMV
                     CAYLAAALLVGLSANALLGWWWADPVTALVIAAVAVREGREAWQGRACCA"
     gene            complement(19845..20306)
                     /locus_tag="VF517_05370"
     CDS             complement(19845..20306)
                     /locus_tag="VF517_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052402.1"
                     /translation="MAPQVDEMAAHDNNEHQMKRIVLPSGKTIEVVYFDHDDFADPRQ
                     APEAQPTAPAEGERELHVCVNCSSDLVYPVQWEEADPRSWHVTLRCPECEWTEAGVFG
                     QDQCDRFDDQLEAGTDALSRDYKRLVTANMSEEIDRFAAALHVDGILPSDF"
     gene            20471..20704
                     /locus_tag="VF517_05375"
     CDS             20471..20704
                     /locus_tag="VF517_05375"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX8052403.1"
                     /translation="MPNIGPLEIAIVLIIVLLIFGPKRLPDLGRSMGRGMREFKDSVT
                     GKDDDKAIEPGETVVKTTADDEPARAAKPADVS"
     gene            20725..21498
                     /gene="tatC"
                     /locus_tag="VF517_05380"
     CDS             20725..21498
                     /gene="tatC"
                     /locus_tag="VF517_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX8052404.1"
                     /translation="MARKIRPVAHDDRLSLVEHLEELRSRLMVALLAFGIAFAVCFWQ
                     DERLISFVNKPLPDGNEPLTLSPTEPLLTTMTIAGYGAILIAMPVVLYQIYSFVLPAF
                     SPTERKVALPLLLMVPFLFVGGAAFGYFVVMEPALKFLLNFNSSEFQTEVRARDYYSF
                     VSLSLLSIGILFQIPVGVLGLTKLGITTPEKLRHNRRYAILGCAVLAALLPTIDPVTM
                     LIEMVPLVVLYEVSILLAVAWGRPSTEVAERLASAEGSS"
     gene            21514..22221
                     /locus_tag="VF517_05385"
     CDS             21514..22221
                     /locus_tag="VF517_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052405.1"
                     /translation="MLFDLRGKRRRAVQVTYAGLALLMAVGLVGAGVGSDVSGGVFDI
                     FTGGDDGDISDANKTTQKKIDSANDKLKANPRDQAALIQVIRGHYTIAASDTDRETGE
                     FGDEGKDELKKASAAWKRYLAAKPEKVDPALATLMMQAYGQTALNEPDNATEAAELVA
                     EERNDATSYIQLVQFASLAGQKRKADLAADKALELAPKGEEKTVKQLLEQARQSAPAA
                     PEGGAQAPAQPPVQGGG"
     gene            22285..22659
                     /locus_tag="VF517_05390"
     CDS             22285..22659
                     /locus_tag="VF517_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8052406.1"
                     /translation="MNFHIDDESIDAETHVIELGGEVDLYTAPEFKERLVQVIEDGKK
                     QLVVDLSKATFIDSTTLGVLVGGVKRLRPSGGSLALVCTDQNITKIFEITGLDRVFPI
                     HASREEALQAVAAGEGAGAESA"
     gene            22785..22857
                     /locus_tag="VF517_05395"
     tRNA            22785..22857
                     /locus_tag="VF517_05395"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22818..22820,aa:Lys,seq:ctt)
     gene            22920..23468
                     /locus_tag="VF517_05400"
     CDS             22920..23468
                     /locus_tag="VF517_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052407.1"
                     /translation="MRLLGLVAMATAAAVALPASAAAQRVPERVPFDPIASTAFGGDS
                     VCTGHQTVTWSRPSGQWVGTLTAQLYCPARMRSVSGECGLGLVARDNVTLAVRSISRN
                     VGGIYVANRIPGTCEPAPIDFDPANPPGETLLGRFDASFYASNGGLAGPTVFCQDKSG
                     DYNRCENDIRFEPGEDLTILYR"
     gene            23657..24703
                     /locus_tag="VF517_05405"
     CDS             23657..24703
                     /locus_tag="VF517_05405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8052408.1"
                     /translation="MAARSLMYLFLVGAIVAGTALAANLGPAVDQSRIAATAGGALGI
                     AIVLFLAYDAAPRWALSLFLLCGSMLIEWAVYAGGDPQSPFLVFYLWIAFYAFYFLSR
                     TQAMLQAAFIGFAYAAVLARDSEPWQTQALRWIVFTIAMMVAGLLVRMLRERIDALIG
                     ELDTASRTDMLTGLLDERGWEEILTKELERARRSGNRVGIVVGQIDGEAPLREAVGGR
                     RADEMLSDVGHRLGKTIRLNDEAARIDDEQFAVVCPYTDETGATIMAERAGSLVRDAM
                     GEPGGPDRTVSFGIASYPKHGTSPEAVMHAARHALGEARGLGGDRAVTFYSVENSIEE
                     RLRSDIDIEVLVSE"
     gene            24714..25502
                     /locus_tag="VF517_05410"
     CDS             24714..25502
                     /locus_tag="VF517_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX8052409.1"
                     /translation="MELACLDGTVQPAAEAWIPATDDGFLRGDGVFEVVRVYDGRPFA
                     LDQHLDRLERSAANLKLGYEVPRAELEEESVQLLGERGGEAFDGCLRLVLTRGGRRLL
                     LTEPLPRGPEHARLGFVTFAPTRLLDAVKSLSYAANMLAGRIARERGFDEALLVTPHG
                     RVLEAPTASLFYVAGDGALCTPPLDDHILASITRDAVLRVTGAEERAVTSDELLGCDE
                     AFLASTTREVQPVAAIEDTELSAPGEHTRAAAAALREHIVGELG"
     gene            complement(25507..25656)
                     /locus_tag="VF517_05415"
     CDS             complement(25507..25656)
                     /locus_tag="VF517_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052410.1"
                     /translation="MTKGVTEAARGAAAVSASVARHAAWYVRYRVDGNKRAQYKRDRP
                     GRARR"
     gene            25747..26541
                     /locus_tag="VF517_05420"
     CDS             25747..26541
                     /locus_tag="VF517_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052411.1"
                     /translation="MAHTLPRHVDLSSPQRATAAARRRVAGAALRSEGWVWAPDRKTV
                     ALGVLAGVTTGAVVVAEVGRIWRRGSAPLPSETDEPLLAAQEAVSETVESAVAGYQEM
                     STRANAAFNLLVSFVVTFVGVRTLTWTLRSRDSIGPFRNVILGRRHIHHYVPGIVLAF
                     VSGAIAILTRNEDIEPKLAIPFGAGMGLTLDESALLLELEDVYWNREGLLGVQVSLAV
                     AALFGALSLGVRFIRRGEVVVLEERVSGPVPGSGPGSAGAPAPRPS"
     gene            complement(26477..27805)
                     /locus_tag="VF517_05425"
     CDS             complement(26477..27805)
                     /locus_tag="VF517_05425"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX8052412.1"
                     /translation="MPGLVLVGAQWGDEGKGKVTDLLAERADVIIRYQGGNNAGHTIV
                     RGDEVFKLHLIPSGILYPGKHCLIGNGVVVDPQVITDEIAGLHDRGIDTGGLKISANA
                     HLIMPYHRLLDHAGEARLGKLQIGTTRRGIGPCYADKALRLGIRVQDLLDEKILRQKI
                     VAAMEPKRQSLRLYAKEPELDLHTMVEEYGRLGHRLEPYISDTTRVVWDALDRDRLVI
                     FEGAQGTLLDIDHGTYPFVTSSNPIAGAACVGAGVGPRDIDAIWGIAKAYTTRVGAGP
                     FPTELENEIGDFIRTAGGEFGTTTGRPRRCGWLDLVALRYAVRINSLTGIAITKLDAL
                     TGLDPLGVAVRYLGPEGASFDQFPYHQSIVHKARGEIVEMPGWHEDITGARSLDDLPA
                     AARAYLDFVEDYLGIPIVLVGVGPGRDQVIRTGAAPELLPTPDPTPAPAR"
     gene            complement(27825..>29213)
                     /gene="dnaB"
                     /locus_tag="VF517_05430"
     CDS             complement(27825..>29213)
                     /gene="dnaB"
                     /locus_tag="VF517_05430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX8052413.1"
                     /translation="SDPPDAAGKVPPQNQEAEVSVLGAILLSEQALDGIQIDVKLRAE
                     DFYRQRHRLIFAAMIRLKEKADPEPVDVLTVSEELNRAGDLDEAGGTAYVHSLPNLVP
                     SAGNVRQYARIVKDHALMRRLLDTTRGIQEDVHGFAGEPKELLDQAEAKLYRIAHDEH
                     SGELRSIEDVLHDELEKLERVSREGIAITGTRSGFNDLDNLTGGFQPGNLIVLAARPA
                     MGKSALVTNIAENASVKYGQPVALFSLEMSETELAQRFIASQAKLNGDDLRKGKVRPD
                     RWPKVMQATEKLAAAPLYIDDSSDLSILDLRAKARRLHARNPLGLVVVDYLQLMRPED
                     SSVGRVEQIGQISRGLKILARELSIPVIAVSQLSRAVEQRNPPEPMLSDLRESGQIEQ
                     DADLVMFIYREEYYKKEESERPGEADILIAKHRNGPVGTVVLTFLNRYPKFMSMTKRD
                     PDAPVPFEADAA"
BASE COUNT         3987 a        10479 c        10393 g         4354 t
ORIGIN      
        1 gtgccgtaga gcgcgacctg ctcggccacg acctcgccga tccggcgcac gccctcgcgg
       61 atcgtgtcct cgtcggagcc ggagaagttc agccgcatcg agttgcgccc gcggccgtcc
      121 aggtaggccg cctcgccggg aacgaacgcc acgttctcgc gcagcgcgcg cgccagcagg
      181 tcggtggtgt cgatgaactc cggcagcgtg acccagatga acaggccgcc gcccggtcgc
      241 gtccactcgg cctggggcgg gaagaactcg gctagcgcgt cgaacatcgc gtcgcggcgg
      301 ctgcggtaga tctcgttgag cgagtcgacg tagtcgcgcc agatgcttcc gtcgaagtac
      361 gcgcgcacca tgagctgcga gagcgtcgag gtgcagaggt cggcgccctg cttgccgagg
      421 ttgatcttct cgaggatcgg cggcggcgcc acgacccagc cgagccggat gcccggcgag
      481 aggatcttcg agaacgtgcc gaggtacatc acgtagagcc cgccgtcgag cgcgcggagc
      541 gtcggctggg ccgagccctc gtagcgcagc agcccgtacg ggttgtcctc gagcacgagg
      601 agctggcgct ccttcgcgat ctcgacgagc atccgccggc gctcgatcga catggtcacg
      661 cccgcggggt tctggaaggt cggcaccgtg tagacgaact tgggccgcct gccctcgcgc
      721 tccaggcggt cgagcacccc gcggagctgc tccacctgca tgccgttctc gtcggtgtcg
      781 atctgcacga cctcggcctc gtacgagtag aacgacggca cggcgccggg gtaggtcggg
      841 ccctcggcga tgatcacgtc gccggggtcg atcagcgtct tcgtgacgag gtcgatcacc
      901 tgctggccgc cggtggtgac gatcacgtcc tcgaggtcga ccttcatgtc ctccgcggcc
      961 atgacctcgg cgatgcagtc cttggtctcg tcgagcccct ccgtcgggcc gtactggagc
     1021 gccttggcgc acgactccgc cgcgatccgg ttgcccatgg cggcgaacgt ctcggtgggg
     1081 aagctggcgg tgtccgggag cccgccggcg agcgagatca cctcggggcg cgccgtgatc
     1141 gccatcaggt cgcgcatggc cgacgacttc atcacgcgcg tacgcgaggc gaacagcccc
     1201 gcatagcgct cgaggtcgcg ctttccggag cgcgggcggg acatggcgcc cttttcgcct
     1261 cccccagtcg ggcccctgcc ttcgtctatc gtcatccggc ccctgagcct accgggcgcc
     1321 acaccccgtc tcggacgatg agtctgttcc tcgacatagg ccaaggcgcg ggcctcgcgg
     1381 gagcaagcgg ggtccggccg tacctgccgc cgctggtggc gggcgccctc gcgcgcggcg
     1441 acacgggcat cgacttcgac ggcacgtcgt acgagttcct cgagcagccg tggttcctcg
     1501 tggtggtgct cgcgctggca gtcgcggcgt acttcgccga ccgcacgcgc ggcacgcgtc
     1561 ccgacgagtc gagcgacccg gtcgcggtcg ggctcggcgc gatcggcgtg gtgctcggcg
     1621 cgctgctgtt cgcggggtcg ctcgcggacg gcgaccacac gagctggccc ggcctgatcg
     1681 gcggcgcggt ggcggcggcg ctgggctact tcgcggtcgc ggccctgttc cgccgcgcgc
     1741 gccggcgcct gcgcgccgcg gaccgctccg gcgagggcgc cccgaacctc ctcgacgcgt
     1801 acgccgacgg gatctcgctc gtgctggccg gcctttcgat cctcgcgccg ccggtcggga
     1861 tcgtcgcgct cgtggtcttc ctcgtgctgc tcctgcgggc gcgcggcggt ggcgggcaga
     1921 agtacgaggg cctccgggtc ctgcggtgag cggcaagagg ccggccggga actcagcgtg
     1981 tgcggctggc gcgcgccggg gcgtggctgc caaggacgca ggaccccgga caatgcagcg
     2041 cattggcggg gtccgaggac gccggcaggc gcgcagccgg cgccgccaga cgtgcgcgcg
     2101 ggggttcccg gttggcctct aagcccaaga agctcgtcct cgccgtcatc gacgccctca
     2161 agccggagat gctcgaccgg gcgatcgagc acggacgcgc gcccgcgctc aaggtgctgg
     2221 tcgagaacgg cacgttcgtg cgcgactgcg tctccacctt cccgtcggtc accccggtgg
     2281 cggcggccac gatcgcgacc ggggtcatgc cggacgagca cgacatcccc tcgatgaact
     2341 ggtacctgcg cggcgaggag cgctacgtgg agtacgggtc gtcgttcgag gcctcgcgca
     2401 cgttcgggct cctgcgcgcg ctctacgaca cggtctacaa cctcaacatg gcccacctga
     2461 gccgcaacgc gccgacggtg ttcgagcgcc tccaggaggc cgacgtgcgc acggccggga
     2521 cgacgtacct gatctaccgc gggcacaagc gccacgcggc gctcgagggc ggcctctacg
     2581 gccggctggc gaaggcgggc gggttccgcc acgcagtctg gggccccgac gagctcttct
     2641 acgccgacct gttcgactcg cgcaagaccg agtgccggtc cacgctcggc atgcccggcc
     2701 agcgcgacca gcactccgcg tgcgtgggcg cgcacctggt cgagcacgac ctgtgcgact
     2761 tcatgctgct gtcgctgccc gacaacgaca cgcactcgca caagcgcgga ccgttcgcgc
     2821 aggtcacgtc gatcgcgtcg gccgaccgcg cgctcgagcg cgtgatgcac gccgccggcg
     2881 gccccgaggc gttcctcgag gaccacgccg tgatcgtgat gtccgaccac gcgcaggtgg
     2941 cggtctcaca ctcggtgaac ctgctggcgg aggcgttcgc ggagtggaag gtgctctacg
     3001 ccgagggcgg cccgagcgac gccgagatcg cggtgtgccc gtcccagcgc tcggcgcagg
     3061 tctacgtgct cgaccaggag cgccgccggg agctgctgcc gaagctcgtg cgcgacctga
     3121 aggtggtgga cggcgtggac gtgatcgcct ggcgcgacgg ccccgaggcg gccgtctgga
     3181 cgcgccgcgg cgagctgcgc ctctcgccgg gcggcgacct gaccgacccg cgcgggggct
     3241 cgtggcacgt cgacggcgac ctcgacgcgc tcgggctggt cgtcaccgac gggcgcgtcg
     3301 acagcgagga gtacccgctc gcgctcgcgc ggctgtggag cgcgctgcgc tgcgcgcacg
     3361 gcggcgacgt gctggtgtcg gcggccacca gctacgagtt cgtggactgg ggcggcgtgg
     3421 cccacatcgg cggcggcagc cacggctcac tccaccgcgg cgactcgcac gcggcgctga
     3481 tcatgtgcgg cgtggacggc ccggcggcgg gcccgccgga ccagtggtcg atcgcggacg
     3541 tggccccgct ggtgctcgaa cacttctctg tagcctgagg atggcgatgc gtcgagactc
     3601 cgcgacggcc gagccgggcg acctggcgct ccacacgcgt gtccgccacg gactgcgcag
     3661 gccgcacaac tggatgcagc tcgtcaagtt ctgcgcggtc ggcgggtcgg gctacgtcgt
     3721 gaacctgtgc gtgttcgcgc tcgcctacaa gctgttcgac ctgcaccatc tcgtggccgc
     3781 gacgatcgcc ttcgtcgtcg ccgtcatcaa caacttctgg tggaaccggc actggacgtt
     3841 cggcgcggcc ggcgcgcgcg agcaccacgc cggcttccag gccgcgcgct tcttcggggt
     3901 cagcgtgctc gcgttccttt tcgcggccgc catcctcgag agcctcgtgc gcttcaccga
     3961 catccggccg atcgtcgcgc aggcgatatc gatcgtggcg gccacgccgc tcaacttcgt
     4021 cgggaacaag atgtggacgt tcgggcgccg cgacgcaggc gcctcctagc cgcgctcctc
     4081 gccctggcgg cgattctcgc ggccgcggcg ccggcggccg cgcaggacct gcgccggccc
     4141 gcgtcgcaga ccgacccgcc gccgttctac gagctctcgg cggcgcaggc gcgggatgtg
     4201 gcggatggcc gcgccgaggt gcgccgcgag cgcgagcggc gcggggcgct ctacggggtg
     4261 gcctacacca agggcggccg gctctggcag gtgagctact tcgccggcgg ggtcgagcgc
     4321 gtgcaggtgg tggtggacga ccgcgatggg cgcgtgctcg aggcctggac cggcaaccag
     4381 gtgcgctggc gcatggcgcg cgggtacgag ggcgcgttcg ggcgcacgct caactcgccg
     4441 tgggtctggc tgccgctctg cctgctgttc ctgctgccgt tcgtggaccc acggcggccg
     4501 ttccggctgc tgcacctcga cctcctggtg ctgctcgggt tcagcgtctc gcacctgttc
     4561 ttcaatcgcg gcgagatcgg cgtctcggtg ccgctcgtct acccggtgct ggcgtacctg
     4621 ctggtgcgga tgctggtggc gggactgcgc ggcgcgcgcg ggcgtgccgg gccgctgctc
     4681 ccgctgttcc cgatggcggc ggtggcgctc gggctggtgt tcctggtcgg cttccgcgtc
     4741 gcgctgaacg tgacggactc gcgcgtgatc gacgtcggtc agtcgagcgt ggtcggcgcg
     4801 gatcggatca ccgacgggcg cgagctgtat ggccagccgt tctccccgac cgaccagcac
     4861 ggcgacacct acggcccggt cacgtacctg ctgtacgtgc cgttcgagct cgcgctcccg
     4921 tggaaggggc ggccggaccc ggcggccgcg cacgcggcgg cgatcgcgtt cgacctgctc
     4981 acgctgatcg ggctgctggt gctcgggcgg cgggtgcgcg cgggaccggc cggctggacg
     5041 ctcggcgtgg cgctcgccta cgcgtgggcc gccaacccgt acacgctgtt cgtgatgcag
     5101 tcgaacgcga acgacagcgc cgtggcgatg ctgctcgtgt gggctttggt cgggatcgcc
     5161 tctgcgccgg cgcgcggggc gttcgtggcg ctggccgcgg cgtcgaagtt ctcaccggcg
     5221 gtgctcgtgc cgctgctggt gcgggctccg gccgcgcgcc gctccaactt catcatttac
     5281 ggggcgaccg tcggcctcgt cctcgttgcc gcctttctcc cgttcgtccc cgacggcggt
     5341 tttcgtgaga tctacgaccg cacggtcggc ttccagctcg cgcgcgagtc gccgttcagc
     5401 atctgggggc aggaggacgc gctcgcgccg ctgcacaccg ctctcaaggt cgccgccgcg
     5461 ctgctcacgg tggcgatcgc cttcgtgccc cgctgccgcg acacgatcca ggtcgccgcg
     5521 ctggccgcgg cggtggtcat cgcggtccag ctggtggtga cgcactggtt ctacctgtac
     5581 gtcgtctggt tcctgccgct cgtgctggtg gcgctactcg ccccgctcgc cggcgggcgc
     5641 cgggaagcgc tcggcccagg gctcgagctc cacgcccgcg acgttgatca cctgggcgat
     5701 cagccggtac cagccggcgc acaccaccag ctccagtagc tgcggcgggt cccactcgcg
     5761 gacgagctcg gtccagaggg tgtcggagac cgtggcggag tcgtacagct catcgacgag
     5821 gcggatcagg aggcggtcgc gcggctcggt ccatgccggg tcgtcggcgg acccgtgagc
     5881 ggtcgcggcg atctgctcgt cggtgagccc gagcgggcgg ccgaacgcga ccgcatgcac
     5941 gccccactcg tactcggcgc cggcgcgggc ggtcgcgcgg tggatcacga tctcgcgctc
     6001 gcgcggctcc acggtcgggt ggccgagcag cgccgcgccg agcgggcgca tccgcgcggc
     6061 cagctcgggg tggatcatca gcgtgcggaa cagcttcagg ggctcgaccg cggcgcccgg
     6121 cggcatccac ttgcgcagcg actcctcgac gtccggctcg tacggcggct cgatcggggg
     6181 gatgcgcggg ggcgtggtgg ggatgcgcgg aggcgtcgtc gccatcgtct acctttctgc
     6241 ttcgatttcc gaagcagctt acaccccccg tcccggacgg cccgcccgcg gctcgaccag
     6301 cggcgagccg atcatggccc tgctcgatct gctcggccgg cgctgggcgc tgcgcgtgat
     6361 ctgggagctg cgcgacggac cgttcacgtt ccgggagctc caggagcgct gcggcggcgt
     6421 ctcgtcgtcg gtgctcgccc agcgactggc cgaactgcgc gaagccggca tcgcggacga
     6481 ggggaacgtg ctgacggccg aggggcggaa gctgctcgag gcctacgagc cgctgggcgg
     6541 ctgggcccgc cgctgggcgc ggcgagcggg ggccgagccg gcgcgacgct agaccgcgct
     6601 ggagcggagc cggcgcgacg ctagaccgcg cggggcgccg tcccgctgct agaccacgag
     6661 cagccggcgc agccgcgcgc ccgtctcgcg cggctccgcg atctgctcgt cggtcacccg
     6721 gccgacccgg aaccccgcga tctggagctc gatgtcgcgg acgcggtcgc cctcgaaggc
     6781 gcggcggtgg ccgtgggtcg cgtagtggtc gatctcgacc accaccttgc gatcgagcca
     6841 cacggcgtcg agctcgtact cgccgaacgg cacgttcatc gttggcagcg gcaggccctc
     6901 cgcgcgcacg aggtcgagga accgcgcctc gacctcggac cggatcaacg ggaccggacc
     6961 ggtcgcgtcg ccgacgagcg cgcggagcgg cttcgcgccg cggtgcccgc gcgcatcggc
     7021 gagcaccctc cacacgtcac ggaggtcgag cacccgcctg cgctcggatt cctcgtacgc
     7081 gcggcgcaac gcccgcggcg gctcggtggc ggcgatgtcg aggagcgtgc gcgcgacgct
     7141 ggtgcacggg atgccgtcga cacgcgtccg gtccccctcg gcgagctgcc gcgggcggtg
     7201 caggacgatg ccggcgcgtg acgccccact ccgtccggtc acggtcacat ccgtgcgcgg
     7261 gcggttgtcc gacaggacct cccacagcca cgccgccgtg cgatggctcg ccagggcgcc
     7321 gggcccgtac gcgagcaccg ctgccatgac gcgcccccgc ttcgtcaccc tgctgtgacc
     7381 cacggcgtac acaccgcgat aaagggggtg gagccggccc gcccgcagcc gcgccttgat
     7441 ctccccgcgc gtccgcccct cggcgaggag ctgccaggcc gcgaccaccc cgtgctggcg
     7501 ctccgcgagc cctgccacca acccgtcaac tgggccatct tttggtcgta tttgacacct
     7561 aacttcgccc aggtccggca tggcgccgac tctgccagcc gtggtgttac aggtgggtca
     7621 ggagcgttac gggtctgtga cgcccgtgtg acgaatcagg cgcgcggggc ctcgaccagg
     7681 agctggcgga tcccggtcgc gcgcccgccg tcgtcggcgt cgatcaggac cgccatcagc
     7741 cagggatcct cctcgctcgt ctcgaagcgg atcggcatca tggtccggaa gcggtggagc
     7801 gcctgctcct tcttcacgcc gatcacgccg ccgcgcgcgc ccgtcatccc cacgtcgctg
     7861 atgtaggccg tgccgccggg cagcacgcgg tagtccgccg tcggcacgtg cgtgtgcgtc
     7921 ccgacgcacg cggtcacgcg cccgtccatg tgccagccca tcgcgacctt ctcggacgtg
     7981 gcctcggcgt ggaagtcgag caggacgtgg tcggcctgat cgcggagctc ggccacgatc
     8041 gcgtcggcct cggcgaacgg cgagcgcgcc gcgtccacga agaccgtgcc gagcaggttg
     8101 gccacgccga gccgcacacc gcccttctcg accacggtga agccgcggcc cgggttgccc
     8161 ttcgggtagt tcgccggacg gatgatgcgg tcgtcgcggt cgaggaaccc gtagacgtcg
     8221 cggtggtggt aggtgtggtt gccgagcgtg accacgtcca cggcgagctc gtcgagcagc
     8281 tcgtgcccga tccgctccgt gatcccgagg ccgccggcgg agttctcgcc gttcacgacg
     8341 acccagtccg gctcgtgctc ctcgcgcagt cccggcagca gatcggagag cgcgcggcgg
     8401 ccgggcttgc ccacgatgtc gccgacgaag agcagtctca ccggccggta ccgtagcccc
     8461 gatgcaagag cccgcagccg cggcggtcct cgaggcccgc aatctcgaga agcgcttcgg
     8521 cgagcgacgc gctctgaagg gagtctcgct caccgcggta tcgggcgacc tcgtcggggt
     8581 gatcgggcca aacggggcgg gcaagaccac gctgctctca ctgctcgccg gaaacgctca
     8641 acccgacgcg ggacagatag tgaccgcgcc ggagaaggtc ggctgggtgc cgcagcagca
     8701 gtcgctctac gggaagctga cggtcgagga gaacctgcgg ctgttcgcgc gactcgagcg
     8761 ctgcgaggac gtggacgaga ccgtcgcgcg catgctcgag cagaccgcgc tccaggagcg
     8821 cgcggacgac caggtcaaga cgctctcggg cggcaaccgc cagcggctca acgtcgcgat
     8881 cggcctcctg ggcgaccccg ccctgctgct gctcgacgag ccgagcaccg cgctcgaccc
     8941 ccgccagcgc gaccgcctct gggagttcgt caccggcctg agcgacgagg gcaccaccgt
     9001 catcttcacg acgcacaatg tccaggaggc cgagcgccac gccacccaac tcgtcgtcct
     9061 cgccgacggc gagcggctgt tcgacggctc gccgagcgag ctgcacacgg tggccgaggg
     9121 caccgacttc gagtccgcgt tcgtcgcatt cctcgcggcc agggggcact gaccgtgcgc
     9181 tggctgctgg ccaaggacct ccgcatcctc cggcgctcgc cgctgctggt ggcgctgctc
     9241 gtgctctacc cgatcctgat cgccgcgctg gtcgggtttg ccgtgacgag cggcccaagc
     9301 aagccgcgcg tggccgtgct gaacgagatc tccgactcgg cgcgcacgat cgacctcggc
     9361 ggccagaagg tcgacgtgaa gaaggaggcc cgtccgctct tcgattcgat cgagcaggtc
     9421 aaggtgaagg accgcgagga ggccgagcgg cgggtgcgcg agggcgacgt gctcgccgcg
     9481 ctgatcgtgc cgagcacgat cctcgacacg ctcgaggcgg cgacccaggg cggcagtggc
     9541 tccgcgcacg tggaggtgct ctacaacgcc gaggacccgg ccaagcgccg ctacgtcgag
     9601 aacacgatca aggcgcgcgt gcaggaggcg aacgcggcgc tggcgaagcg cttcgccgag
     9661 gccggagcgg gatacatcga ggtgctggcc agcggcggga agatctcgat cgcgagcctt
     9721 cagatcgacg tgctcgggct cggaccggcc gccgcgatcc tgaaggacgt ccgcgacgcg
     9781 ctgcccgcgc gcgcgcccga gcgcaaggag ctcgaccagg tgatccagtt cgcgcagctc
     9841 ggcgccgaga acctcggcct ggccgaaacg ctgctcggcg cggtcggcac gccgatcgag
     9901 gtggacagca aggtggtcga gggcgccgac acgccgctcg gcgcgttcgc cgccgccgtc
     9961 gccgcggccg tcaccctcat gttcgtcacg ctcctgctcg cgagcggcgc gctggccctc
    10021 gagcgcgagg agaacaccta ccgccgcctc gtgcgcgggc tcgtctcaca cacggcactg
    10081 ctcgtggaga aggccggcct agccgcgctc tgctcgctgg cggtcgggct cgtcctgctc
    10141 gtcgggctgt cgttctccgc ggacctcccg tggggacgct tcccgctctg gctgctcgct
    10201 ctgggcgccg gggccctggc gttcggagcg ctcggggtgg ccatgggcgc ggtcgcgcgc
    10261 gaggtgcgcg cggcgtcgct cctggcgttc atggccgcgc tgccgatcgc ggtgctcggg
    10321 ctcatcccct cgggggccgt gggcagcggc ctctacgacc tgctgcgcgc gatctcggcg
    10381 gtcttcccct tccgcgcgac cctcgacgcg ctccagtcgg cactaggccg gtccggcgac
    10441 atggccgcgc cgctggtcca cctcgccgtg ctcacgctcg cctacggcgt cgcggcgcgg
    10501 ctcgccctgc gccgcttcgc catcgactag aggccggcgg cgtcagccgc tgacgagcgt
    10561 ggtgacgccg tcctcccaca cgtaggcgtc ctccgcgacg tccgtgtccg ccgccaccag
    10621 cgccttcttc gagaagaagt acacgcggct gccgtcgtcg gacgggtaga ccttcgtgtg
    10681 ctcgctgtcg gagacgttcg cgtggtcgtc cgccggaccg ctcgtcacca gcctgaccgt
    10741 gccgccgacg ctctcgtaga cgtcgcggcg gttgtcggtg tccgccgggt ccacgccctc
    10801 ctgggtcacg aagaagacgt gtgtgaggtc gcgcgagctg gcggcaaggt cgaggtgttc
    10861 gaccggggtg cccggggtca gcagcttgaa ctggccgccg gcgcgctcgt ataggtcgtt
    10921 cttcgagtcg gtgtccgccg gaacgagcgc ggcaaccgag cggaagacga gccgcgcgcc
    10981 gtcgggcgac atccagccgt cgtagccctg ggcaacgtgc tcgaggctcg accccgtccg
    11041 caggtacatg tcccaggtgc tgttggtgtc ggtcgaggcc agggcctgcc cggcgccgaa
    11101 gaacaccgat cccccgtccg tcgtctgcgc cgccctgaag atcgtgcacg gctccgggcc
    11161 gccgtggcca gctgggatcg ggcacggctg cacgaggccc agccggttcg cggtgagcag
    11221 gcgcgtcttc ccgttcctgc gctggtagag gtcgagtgtg ccgtttgtgt cgcccagctc
    11281 gaacttcgca tccgtcatgt agatgaacga gctgccgtcg tcggacgcgc cgccggcgat
    11341 ccaagccgaa tgcggtgcgc tgccggtgtc caaggtgccg gtcgtgacga gcttgaccgt
    11401 tccgcccgag cgctcgtaga agtccccggc gccgtcggtg tcgttcgaga cgagcgcctc
    11461 gtccgtgccc tcgaacaccc gcgtaccgtc aagcgacgcc ccgacgaagt agtggccgtg
    11521 cggccccgag ctgccggccg ggctggccga caggagcgtc gtgacgccgt tcgagcgcat
    11581 gtagagatca cgctcgccgt cggtgtcctc gggcaccagg tccgcgctcg tcccgaagat
    11641 cacgcggctg ccgtcggggg agatgtgcgc gagctcgtac cagggcagcg gaccggggtc
    11701 ggtcgggccg gtcgtgatca gcgtggtctg cccgcgcgag cgctcgtaga ggtcgtagtc
    11761 gctgtcggtg tcggccgcgc tgagctgctc ggccgtggag aagaacacac gggaggcgtc
    11821 cgtggagatc gcctgcgcga acgctccgcc ggcggtgccc acggacgcga gctcgttcac
    11881 gccgcccgcg cgcacgtaca cgtcggccgc cgtgtcggtg tccgacgcgg tcagggcctc
    11941 cgtcgtgctg aacacgaacg cgccgccgtc cggcgtgacg cgccggaaat cggcactcgc
    12001 cggcgccgcc cagctcgcga tcgcgacgac tgcaaccgcc gcccccctag cgaaaagcct
    12061 catctcagaa cgccccccag cgtcggtccc caccgcgtgc cggtgcagga tgctacaccg
    12121 cgttggcgcg tcggcgccgg actccgcgta cccccaagcg cgcggccata cactcatatc
    12181 gatggcattt ccagcgaccc gcatgcgcag gctgcgccgc accgggctgc tgcgcgggat
    12241 ggtccgcgag accgagctga ccgccgcgca cctcgtctac ccgctcttcg tggagcttgg
    12301 cacggacgcg cgcacgccga tcgacgcgat gccggggatc gaccgcctgt cgatctcgca
    12361 cgcggtcgag gaggccgccg ccgcgcacga gctcggggtc cccgccgtgc tgctgttcgg
    12421 gatcccgtcg cacaaggacg agcaggcgtc cggcgcctac gacgccgagg gcgtggtcca
    12481 gctcgccacg cgcgcgatca aggacgcgct gcccgacctc gtggtgatga ccgacgtgtg
    12541 cctgtgcgag tacacctcgc acggccactg cggcgtggtt cgcgcggacg gcgccgtgga
    12601 caacgacatg tcgctggagc tcctggcgaa gaccgcgatc tcgcaggccg ccgcgggagc
    12661 cgacgcggta gcgccaagcg acatgatgga cggtcgcgtc ggcgcgctgc gcgcgcagct
    12721 cgacgccgag ggccacagcg agacgccgat cgtcgcctac tccgcgaagt tcgcgtccgc
    12781 cttctacggg ccgttccgcg aggcggccga ctcggccccg gcgttcggcg accggcgctc
    12841 ataccagatg gatcccgcca acgccgacga ggccgtccgc gaggcgctgc tcgacgtgga
    12901 ggagggcgcc gacgtggtga tggtcaagcc cgctctcccc tatctcgacg tggtgcgccg
    12961 cgtgaaggag gccacccagg tgcccgtggc ggcctacaac gtgagcggcg agtacgcgat
    13021 ggtgaaggcc gccgcgcagg ccggctggct cgacgagcgg gcgaccgtgc tcgaggcgct
    13081 cacctcgatt cgccgcgccg gcgccgacat cgtcatcacg taccacgcca aggatgtggc
    13141 aggctggttg tcgtgaccag cgcggtcccc aagctccgct cgcgcaagga cggcgccgcc
    13201 gttccgctcg acgacctcga caagcggctg ctcaacctgc tccagggcag cttcccgctc
    13261 gagccgcgcc cattcctgcg cgtggccgag ctggccgagg tggccgagga ggaggtgctc
    13321 gcgcgcaccc agcggctgct cgacgagcgc atcatccgcc aggtcacgcc gatcttcgac
    13381 acgcgcgcgc tcggctacaa gtcgatgctg gtcgcggcga ggatcgaccc ggagaacccg
    13441 caccgcgccg cgaaggtggt caacgcgcac ccgggcgtct cgcacaacta cctgcgcaac
    13501 cacgacttca acctgtggtt cacgatcgcc gtcgagccgg actcgcggct cggcctggac
    13561 gggacgctcg acgcgctcgc ggaggaggcc ggcgcggagt cggtgcgcca gcttccgacg
    13621 ctgaagctct tcaagatccg catggacctg gagatggagg gcgacacgaa ggcgctcgcc
    13681 cgcgcggccg aggcgacgga gcccgccgag accgagccgc agccctacga cgacttcgac
    13741 gtggccgtga tccgcgagct ccagggcgac atgccggtcg tgccggagcc gtacgcgccg
    13801 gccgcgcggg cgctcggcat cccgcagcag cagctgctcg accacctcga cggcatgcgc
    13861 gagcgcaggc tgctgcgccg cgtcgcggcg atcctgttcc accgccgtgc cggcttctcg
    13921 gccaacggca tgggcgtctg gcgcgtgccc gacgaccaga tcctggaggt cggcaagcgc
    13981 atggcctcgt tccgcgggat ctcgcactgc tatcagcggc cgacctacgc ggactggccg
    14041 tactcggtct tcaccatggc ccacgggcgc tccaaggagg agtgcgacgc gatcctcgac
    14101 gcgatcgccg aggacaccgg catcgaggag cgctcgacgc tgtactcctc gaccgagttc
    14161 aagaaaatcc ggctcctcta cttcaccgac gagttcaagg agtgggaggc ggcacgcctc
    14221 tagccgtgac ccgctcgcag gagctgtacg agcgggcact cggcctcatc ccgggcggcg
    14281 tcaactcgcc cgtccgggcg atgcgctcga tcgggcgcga cccgatcttc gtggccgagg
    14341 cgagcggcgc ggagctgatc gacgccgacg ggaaccgcta cctggactgg atgcagtcgt
    14401 gggggccgct ggtcgcgggc cacgcgcacc ccgccgtgat cgccgccgtg cgcgacgcgg
    14461 ccgagcgcgg cacgacctac ggcgcggcca cggagcggga ggtggacctc gccgcggaga
    14521 tcgtccgccg cgtgccctcg gccgagatgg tccgcatgac ctcgtcgggc accgaggcct
    14581 cgatgagcgc catccggctg gcccgcgcgg cgaccggacg cgaccggatc gtgaagttcg
    14641 ccggcgccta ccacggccac gtggacgggc tgctcgccga cgcgggttcc ggactggcca
    14701 cgcagggcat ccccgcgagc ccgggcgtga ccgccgcgca ggccgccgac accgtcgtcg
    14761 tgccgtggaa cgatcgcgac gcggtggagc gcgcgctcgc cgagcagccg gtggccgcgc
    14821 tcctgtgcga gccctacccg gcgaacatgg gcctcgtccc gcccgccgtg ggcttcctcg
    14881 ccttcctccg cgacgcgacc gccgcgagcg gcgccctgct cgtgttcgac gaggtgatct
    14941 cgggcttccg cgtggcgccg gggggagcgc aggggcgcga gggcgtcctg ccggacctca
    15001 cgatcctcgg caaggtcatc ggcggcgggc tgccggcggc ggcgtatgcc ggaccggccg
    15061 agctgatgga gcgcgtggcg cccgcgggcg acgtgtacca ggccggcacg ctctcgggga
    15121 acccgcttgc ggtggccgcc ggtctggcga ccctgcgcct gctcgacgcg gacgcgtacg
    15181 cacggctcga cgccaccacc gccgagctgg ccgcggcgct cgaggccgcg gcgcgcgacg
    15241 ccggggtgcc ggtccaggtc gcgtggacca ccggcctgct gacggtcttc ctctcgaatg
    15301 agccggtgcg cgactacgcc ggcgcgaagg cgtgcgacca cgaggcgttc gccgcgctct
    15361 gccgcgggct gctcgaccgc ggcgtctacc cgccgccgtc gcagtacgag gcgtggttcc
    15421 cgtcgctcgc ccacacgggc gagcacgtcg agcgcaccgc ggcggcggcg cgggaggcgt
    15481 tcgcggagct atgagcctcg cggcggacat cgaggcgggc gccgaggccg tcgggcgcgg
    15541 cagcgcgggg accagggagt gggtcctcga ggcggtccgc gagggctacc tcctccacta
    15601 cggagagccg cgcgcgttcg acggcgacat ggaccaggac ctgcggctgc tcgccggcga
    15661 cacgctctac gccctcggcc tggcgcggct cgccgcgacc ggcgatctcg aggccgtcgg
    15721 cgagctggcc gacctgatct cggcctgcgc ccatgccgag tcggagggcc ggccgaccgg
    15781 cgacctctgg gacgtctcgg cccgccgcct ggcgcgaccc aacccgccgg aatagcctcc
    15841 attccgcatc cagggcgcgc ttcgcggccg cgcgccagcg acccgcgata tcgtgccgtg
    15901 taagcccgtg gccgatccgt caaagcgacc ccacgaccgt aagtccccgt acaccgtcga
    15961 tcgcggcatg ccgggtggct tcgagggtga gaccgtcacc cgcaggaccc tgttcacggg
    16021 aggtgcgttg gcggccggcg gcatcatgac ggccgcgttc acgctcccgg cgctcggctt
    16081 cgcgcttggt ccgatgttcg agcgcaccga ggacccgcgc ttcaccgacg tcggctcggt
    16141 gcgcgacttc aacacccaga cctacgtccc gcgcgtgatc acgctggtcg acgacgtcgg
    16201 cgaggctggc aagaccaccg tctacgtccg ccagttcgac gcccgccgcg acaaggacaa
    16261 gctcgagcag ccgttcgtcg cgatctccac ccgctgcatg cacctcggct gcccggtccg
    16321 ctacgtgggc gcctcgcaga agttcatctg cccgtgccac ggcggcgtct acgagttcga
    16381 cggctccgtc ggcggcggcc cgccggtccg gccgctcgac cgcttcgaga cccgcgtgcg
    16441 caacggccgc gtgcagatcg gcccccgctt ctcgctgaac acaaagctcg agcgcaagaa
    16501 ggtccgcgac ccgtcgaacc acctcgacgg gctgtggcag tacatctacc cgtcgaggcc
    16561 caccgtatga agctgctccc cggcccactt cgccgcaaga acggcaagaa cggcaccaat
    16621 ggcaacggca acggcaacgg tgccacccgg gtcatgcccg gcgacctgcg caacggtcac
    16681 gaggagcgca ccaaggagcg ggagaaggag gccgaccgca agcgccagct cgacgccgtc
    16741 aaggagacgg gcatcaacgt cgtcggcttc ctggacgagc gcaccggcgg cggcccgttc
    16801 atgcggttct tcctgtaccg gaaggtgccg ccgatcaact ggttctacac gctgggctcg
    16861 gcgacgatgt tcgccttcgt ctcgcaggcg gtgaccgggg tcttcctcgc catgtactac
    16921 cgcccgtcga gcaacgaggc ctacgagtcg gtccagcaca tcaccaacga cgtcttcgcc
    16981 ggcgagctcg tgcgcggcat gcaccgctgg ggcgccacgg tgatgatgat cctgatcttc
    17041 atgcacatgg gccgcgtgtt cttcttcggc ggctacaagt acccgcgcga gctgaactgg
    17101 gtgatcggcg tggtcctgct ggtgctcacg ctgatgatgg gcctcaccgg atacctgctg
    17161 ccgttcgacc agcggtcgtt ctgggcgacg gtcgtggcgg tgaatctgaa ctcgtccgga
    17221 ccgatcatcg gcccgtacct cgcggacttc ctgcgcggcg gagccgagtt cggcgccaca
    17281 accatcagcc gcttctacgc gctgcacatg ctcctgatcc ccggcctgat catggccctg
    17341 atcggcgcgc atctctacct cgtcgtgaag ctcggcacga ccgccccgcc gtggctgaag
    17401 gccgagaagg cgcgcgacct ggccaaggaa gagatctagg ccgtgaacgg acgcgagcgc
    17461 gagcagtacc tccgcgagta ccagctgctc aagaacaaga aggggaagcc gttcttcccc
    17521 tacgcgatcc tgaaggactc ggcgatggcc tgcatcgtcc tgttcgtgat catcgcgatg
    17581 gcgatcatcc tcggcgccga gctgggcccg aaggccgacc cgacctcgac cacggtcacg
    17641 ccgcggcccg agtggtactt cttcttcctc ttcgagctgc tgcgggtcat caagccgccg
    17701 gcgctcgtct tcgtggccac gatcggcatc cccacgatct ggctcgtgct gctgctcctg
    17761 ctgccgttct acgaccgcaa cccggagcgc cacccgctca agcggccgat cgcgaccacc
    17821 gccggcatcc tcacgatcct gacgatggcc tacctcacga tcgagggcgc gctggccggc
    17881 tcgccgaccg agatcgagat gaaggtcgag aagcggctcg agccgggcag ggccgtggcg
    17941 gcgtcgtccg gctgcctcgg ctgtcaccgg atcggcgaga acggcaacac ggggcccggc
    18001 ccggcgctca cgaacgtcgg cgagcgcctg ccgcgccagg ccatcgcccg gacgctcgtg
    18061 aacccgacag cgccgatgcc gtcgtactcg cagctgcgcc agaacgagcc ggagcagttc
    18121 aaccagctcg tcgagttcct gagctcgctc cgggagtagg acgcatgccc gccaaggccg
    18181 ccgagcagct cggcgacgtg ctcgcgcgag gcggcccgcg gctccgcgcg ctgctcgacg
    18241 cgaccgagct ccgcctcgcc ggcgtggccg agggctacgg cgacgccctg tccgcgcacg
    18301 tcgcgcagac gctcacggcg ggcggcaagc ggctgcgccc gatcctcgtg ttcataaccg
    18361 gcgggagcgc cacaggcgag gcgctcgtgg ccgccggcgc cgccgtcgag ctcctgcaca
    18421 tggccacgct cgtgcacgac gacgtgctcg accgcgccga gctgcgccgc ggcacgcgga
    18481 ccgtgtacgc ccagggcggc cgcggggccg ccacggcgac cggcgatctg ctcttctcgc
    18541 gggcgttcgc gctgctggcg gccaccggtg agcgcgaggc gctgcgcgcg ctctcggacg
    18601 cctcctcggc gctggcgcgc ggcgagctgc tccagcgcga ggacgcgtgg tcgcccgcgg
    18661 tcacgccgga gcgctacctc gagcgctgcc ggctcaagac cgcaccgctg ttctccgcgt
    18721 cgtgccggct cggcgcgatc ctcggcgacc ccggcccgga ggccgccgac tccctggcgc
    18781 gcttcggcga ggggatcggc ctcgcgttcc agctcttcga cgacgtgctc gacgtgtcgg
    18841 ggcccccgga ggtgaccggc aagccgcgcg ggaccgacct gctcgacggg acggtgacgc
    18901 tgccgctgat cgtcgcgcgc gagcgagacg ccgacctgcg cgcgctcgac ctgcgcggcg
    18961 cggcgcacga tcacgcgagc gcagccgcgc tgtgcgatcg gatcgcggcc acgggcgcgc
    19021 tcgaggagac gcgcgaccag gcgctcgacc acgtgcgccg cgcgaaggcc gcgctcgagg
    19081 acgtggagct cgacgacgct cagcgcgagc tgctcgaggt catcgcgaac ggcgtggccg
    19141 accggcaggc ttagcgggcc ggcgccggcc cccgcgggct acgcgcagca ggcgcggccc
    19201 tgccaggcct cgcgcccctc gcgcacggcc acggccgcaa tcacgagggc ggtgacggga
    19261 tcggcccacc accagcccag cagcgcgttc gccgacagcc cgaccagcag cgcggccgcc
    19321 aggtaggcgc agaccatgtt ctgccgcccc tcggccttcg tcgccgacga gtgaagctcg
    19381 tgcgcgagcc gcaccttcgc ccgcgcgagc ggcggcatcg tgatcagcgt ggccaccgcg
    19441 agcacgatgc ccacggtgct gccgtcaggc cgctcacctc ccaatagccc gcgacccgcc
    19501 tcgacggcca cgtaggcggc caggacgtag aagctcaccg cgatcagctt ctgggcgctg
    19561 cgctcggcgc cctcggacgc gctgcgcacg gccgcgaagc gccacagcag cacgaagccg
    19621 gccatggcct cgacgaccga gtcgatcccg aagccgacca gcgcgatcga accggcggcg
    19681 acgccggccg cgatcgccac cacggcctcg atcccgtgcc acgccatgcc gagccaggcg
    19741 agcgccttgg cccgccgcac cagtgtgtcg cggccgggca gcggaggtgc tgcgacggac
    19801 gaggtggtca cgatctcagc gtaacccgcg ccgcggcgcg cgtcttagaa gtcgctgggc
    19861 aggatgccgt cgacgtggag ggccgccgcg aagcggtcga tctcctccga catgttcgcc
    19921 gtgacgagcc gcttgtagtc gcggctcagc gcgtcggtgc cggcctcgag ctggtcgtcg
    19981 aagcggtcgc actggtcctg gccgaacacg ccggcctcgg tccactcgca ctcggggcag
    20041 cgcagggtga cgtgccagct gcgcgggtcc gcctcctccc actgaaccgg gtagacgaga
    20101 tcggacgagc agttcacgca gacgtggagc tcgcgctcgc cctcggcggg ggccgtcggc
    20161 tgagcctcgg gcgcctggcg cggatcggcg aagtcgtcgt ggtcgaagta cacgacctcg
    20221 atcgtcttgc cgctgggcag gacgatgcgc ttcatctgat gttcgttgtt gtcgtgtgca
    20281 gccatctcgt ccacctgtgg ggccacgtat cggcagtgga cggctgcact tgaggggcgg
    20341 tcgccaaacg ttcgaccgga ccgctgcatg cgcggcccgc tgatccagtc gagcgggacc
    20401 tccgtagatg gtggagagca ccgggggcag taagccctac catggttgct ctcccccact
    20461 ggaggaccgg atgcccaaca tcggaccact tgaaatagcc attgtcctca tcatcgtgct
    20521 gctgatcttc ggaccgaagc gcctgcccga cctcggccgc tcgatgggcc gcgggatgcg
    20581 cgagttcaag gactcggtca ccggcaagga cgacgacaag gcgatcgagc cgggtgagac
    20641 ggtcgtgaag acgaccgccg acgacgagcc cgctcgcgcc gccaagccgg ccgacgtctc
    20701 ctagcgtcgg tagccgctag cgccgtggcc cggaagatcc ggccagtcgc gcatgacgac
    20761 cggctcagcc tcgtcgagca tctcgaggag ctgcgctcgc gtctcatggt cgcgttgctc
    20821 gccttcggaa tcgcgttcgc cgtctgcttc tggcaggacg agcgcctcat ctccttcgtc
    20881 aacaagccgc tgcccgacgg caacgagccg ctgacgctca gcccgaccga gccgctgctc
    20941 accacgatga cgatcgccgg ctacggcgcg atcctgatcg ccatgccggt ggtcctctac
    21001 cagatctact cgttcgtcct gccggcgttc agcccgaccg agcgcaaggt ggccctgccg
    21061 ctgctgctga tggtcccgtt cctgttcgtc ggcggcgccg cgttcggcta cttcgtcgtg
    21121 atggagcccg ccctgaagtt cctgctcaac ttcaactcga gcgagttcca gacggaggtg
    21181 cgggcgcgcg actactacag cttcgtctcg ctcagcctcc tgtcgatcgg gatcctcttc
    21241 cagatcccgg tcggcgtact cggcctcacg aaactcggga tcaccacgcc cgagaagctg
    21301 cggcacaacc gccgctacgc gatcctcggc tgcgccgtgc tggctgcgct gctgccgacg
    21361 atcgacccag tgaccatgct gatcgagatg gtcccgctgg tggtgctcta tgaggtaagt
    21421 atcctgctcg cagtggcttg ggggaggccg tcgacggagg tcgcagagcg gcttgcgtcg
    21481 gcggaaggct cctcctagct gcacgcgaga acgatgctct tcgacctcag aggcaagcgg
    21541 cgccgcgccg tacaggtgac ctacgcgggt ctcgcgctgc tgatggctgt gggcctggtc
    21601 ggcgcgggcg tcggcagcga cgtctcgggc ggcgtcttcg acatcttcac cggcggtgac
    21661 gacggcgaca tcagcgacgc caacaagacc acgcagaaga agatcgactc cgccaacgac
    21721 aagctcaagg cgaacccgcg cgaccaggcg gcgctgatcc aggtgatccg cggccactac
    21781 acgatcgctg ccagcgacac cgaccgcgag accggcgagt tcggcgacga gggcaaggac
    21841 gagctgaaga aggcgtcggc ggcgtggaag cgctacctcg cggccaagcc ggagaaggtg
    21901 gacccggcgc tcgcgaccct gatgatgcag gcctacggcc agaccgcgct caacgagccc
    21961 gacaacgcca ccgaggcggc cgagctcgtg gccgaggagc gcaacgacgc gacctcgtac
    22021 atccagctcg tccagttcgc cagcctcgcc gggcagaagc gcaaggccga cctcgccgcc
    22081 gacaaggcgc tcgagctcgc gccgaagggc gaggagaaga ccgtgaagca gttgctcgag
    22141 caggccaggc agtccgcgcc ggcggcgccg gagggcggcg ctcaggcgcc cgcgcagcca
    22201 ccggtgcagg gcggcggcta ggcccgtggg cgcaaccctg ctagaaaccc gtcacaattc
    22261 ccgcaccccg aacgtaggag ccaggtgaac ttccacatcg acgacgaatc gatcgacgcc
    22321 gagactcacg tcatcgagct cggtggtgag gtcgacctct acacagcgcc cgagttcaag
    22381 gagcgcctcg tccaggtgat cgaggacggc aagaagcagc tcgtcgtcga cctctcgaag
    22441 gcgaccttca tcgactcgac cacgctgggt gtgctcgtcg gcggagtcaa gcgcctgcgc
    22501 ccgagcggtg gctcactggc gctggtctgc accgaccaga acatcacgaa gatcttcgag
    22561 atcaccggcc tcgaccgcgt gttcccgatc cacgcttcgc gcgaggaggc ccttcaggcg
    22621 gtcgccgcgg gcgagggcgc gggcgccgag agcgcctagc ccggcgcggc ggagcgccgc
    22681 ttggcggcgg cggctctgcg ccccgcgagg gcggcggctc tgcgccgcgg agggctccta
    22741 tcatgggccc tcaggtccgg ccgcgccgga ccgatataca gcccgggccg ttagctcaat
    22801 tggcagagca ggggactctt aatcccaagg ttgcaggttc gattcctgca cggcccactc
    22861 gagcggcgcg catggccgcc tgacggagta gcttcccggg tgtccacggg gggtgaggca
    22921 tgaggttgct ggggctcgtt gcgatggcta cggccgcggc agtggcgttg ccggcgagtg
    22981 ccgcggcgca gcgtgtgccg gagcgggtgc cgttcgaccc tattgcgagc acggcgttcg
    23041 gcggcgactc ggtctgcacc ggccaccaga ccgtgacctg gtcgcggccg tcggggcagt
    23101 gggtcggcac gctcacggca cagctctatt gtccggccag aatgcgctcc gtgagcgggg
    23161 aatgtgggct tggactcgtc gcccgcgaca acgtcacgct cgccgtgcgg agcatctcgc
    23221 ggaacgtcgg cgggatctac gtcgcgaaca ggatccccgg cacctgtgag cccgcaccga
    23281 tcgacttcga tccggcgaac ccgccgggcg agacgctgct cggcaggttc gatgcgtcgt
    23341 tctacgcgtc gaacggcggc ctcgccggac cgaccgtctt ctgccaggac aagagcggcg
    23401 attacaaccg gtgcgagaac gacatccggt tcgaaccggg agaggatctg acgattctct
    23461 accgctagct cgatgtacgc gggccacgct ccatcccccc cgattagggg tactccagac
    23521 cggtaggcgc ttatttcctg gaaatcggcc cgttcgtgcc gataagacca ggagtcgtat
    23581 ggatccttct attcgcgaga ggctgggcct gcgcccgccc gtccgccccc agggcagagg
    23641 tctggcggag caggcggtgg ccgcccggtc gttgatgtac ctgttcctcg tcggcgcgat
    23701 cgtcgccggc accgcgctcg cggccaacct cggccccgcg gtcgaccagt cccgcatcgc
    23761 cgccacggcc ggcggcgcgc tcggcatcgc gatcgtgctg ttcctcgcct acgacgcggc
    23821 gccgcgctgg gcgctgtcgc tcttcctcct ctgcggctcg atgctgatcg agtgggccgt
    23881 gtacgcgggc ggcgacccgc agagcccgtt cctcgtcttc tacctctgga tcgcgttcta
    23941 cgccttctac ttcctctcgc gcacgcaggc gatgctccag gcggccttca tcggcttcgc
    24001 ctacgccgcc gtgctcgcgc gtgacagcga gccctggcag acccaggcgc tgcgctggat
    24061 cgtgttcacg atcgcgatga tggtcgccgg cctgctcgtg cgcatgctgc gcgagcgcat
    24121 cgacgccctg atcggtgagc tcgacaccgc gtcgcgcacc gacatgctca ccggcctgct
    24181 cgacgagcgc ggctgggagg agatcctcac caaggagctc gagcgcgccc gccgcagcgg
    24241 caaccgcgtc gggatcgtgg tcggccagat cgacggcgag gccccgctgc gcgaggccgt
    24301 cggcggccgc cgcgccgacg agatgctctc cgacgtcggc caccggctcg ggaagacgat
    24361 ccgccttaac gacgaggccg cgcgcatcga cgacgagcag ttcgccgtgg tctgccccta
    24421 caccgacgag accggcgcga cgatcatggc cgagcgcgcc ggcagcctcg tgcgcgacgc
    24481 gatgggcgag ccgggcgggc ccgaccgcac cgtcagcttc ggcatcgcca gctaccccaa
    24541 gcacggcacc tcgccggagg ccgtgatgca cgccgccagg cacgccctcg gcgaggcacg
    24601 cggactgggc ggcgaccgcg cggtgacgtt ctactcggtc gagaactcga tcgaggagcg
    24661 cctgcgctcg gacatcgaca tcgaggtgct ggtctccgag tagtctgagg cgggtggagc
    24721 tcgcctgcct cgacgggacg gtccagccgg ccgccgaggc ctggatcccc gccacggacg
    24781 acggcttcct gcgcggtgat ggtgtcttcg aggtcgtgcg cgtctacgac gggcggccgt
    24841 tcgccctgga ccagcacctc gaccggctcg agcgctccgc ggcgaacctg aagctcggtt
    24901 acgaggtgcc gcgggcggag ctcgaggagg agtccgtgca gctgctcggc gagcgcggcg
    24961 gcgaggcgtt cgacggctgc ctgcggctcg tgctcacgcg aggcggccgg cggttgctgc
    25021 tcaccgagcc gctcccgcgc gggcccgagc acgcgcgact cgggttcgtc accttcgccc
    25081 ccacgcgcct cctcgacgcg gtcaagtcgc tctcgtacgc ggcgaacatg ctggccgggc
    25141 ggatcgcccg cgagcgcggc ttcgacgagg cgctcctggt cacgccgcac gggcgcgtcc
    25201 tggaggcgcc gacggcttcg ctgttctacg tcgccggcga cggtgccctc tgcaccccgc
    25261 cgctcgatga ccacatcctc gcgtcgatca cgcgcgacgc cgtgctgcgc gtcaccggcg
    25321 ccgaggaacg cgctgtgacg agcgacgagc tgctcggctg cgacgaggcg ttcctggcgt
    25381 cgaccacgcg cgaggtccag ccggtcgcgg cgatcgagga caccgagctg tcggcgcccg
    25441 gcgagcacac ccgcgcggcg gccgccgcgc tgcgcgagca catcgtgggc gagctgggct
    25501 aggcgctcag cggcgcgcgc ggccggggcg gtcgcgcttg tactgcgcgc gcttgttccc
    25561 gtcgacgcgg tagcgcacgt accacgcggc gtggcgcgcc accgaggcgc tcaccgcggc
    25621 ggcaccgcgc gcggcttccg tgacaccctt ggtcaagctc gcgacgatcg ggtccccctt
    25681 agatccggcg ggcatcgtat tgaccacatc ggcacagccg cgcgcgggtt gaaggttccg
    25741 actcgcatgg cgcatacact cccccgccac gtggacctct cctcgccgca gcgcgccacg
    25801 gccgccgcgc gccggcgcgt ggccggcgcc gcgctgcgat ccgagggctg ggtgtgggcg
    25861 cccgaccgca agaccgtggc gctgggcgtg ctggccggag tgacgacggg tgctgtcgtg
    25921 gtggccgagg tcggacgcat ctggcggcgc gggtcggcgc cgcttccgag cgagaccgac
    25981 gagccgctgc tcgccgcgca ggaggccgtc agcgagacgg tcgagtcggc ggtcgcgggc
    26041 taccaggaga tgtccacgcg ggcgaacgcg gccttcaacc tgctcgtgtc gttcgtggtc
    26101 acgttcgtcg gggtgcgcac gctcacctgg acgctgcgca gccgcgacag catcgggccg
    26161 tttcgcaacg tgatcctcgg gcgccgtcac atacaccact acgtgcccgg gatcgtgctc
    26221 gcgttcgtgt cgggcgccat cgcgatcctc acgcgcaacg aggacatcga gccgaagctc
    26281 gcgatcccgt tcggcgccgg catgggcctg acgctcgacg agtcggcgct gctgctcgag
    26341 ctggaggacg tctactggaa ccgcgagggg ctgctgggcg tgcaggtctc gctggcggtg
    26401 gcggcgctgt tcggggcgct gtcgctgggc gtgcggttca tccgccgcgg cgaggtggtc
    26461 gtgctcgagg agcgggtcag cgggccggtg ccggggtcgg gtccggggtc ggcaggagct
    26521 ccggcgccgc gcccgtcctg atcacctggt cgcggccggg gccgacgccg accagcacga
    26581 tcgggatgcc gaggtagtcc tcgacgaagt cgaggtaggc gcgggcggcg gccggcaggt
    26641 cgtcgagcga gcgggcgccg gtgatgtcct cgtgccagcc gggcatctcg acgatctccc
    26701 cgcgcgcctt gtggacgatc gactggtggt aggggaactg gtcgaagctc gcgccctcgg
    26761 ggccgaggta gcgcaccgcg acgccgagcg ggtcgagccc ggtgagcgcg tcgagcttcg
    26821 tgatcgcgat cccggtcagg ctgttgatgc ggacggcgta gcgcagggcg accaggtcga
    26881 gccagccaca gcggcgcggg cggccggtgg ttgttccgaa ctcgcctccg gcggtgcgga
    26941 tgaagtcgcc gatctcgttc tcgagctcgg tcgggaacgg tccggcgccg acgcgcgtgg
    27001 tgtacgcctt ggcgatgccc cagattgcgt cgatgtcgcg cggcccgacg ccggcgccga
    27061 cgcacgccgc gcccgcgatc gggttcgacg aggtgacgaa cggatacgtg ccgtggtcga
    27121 tgtcgagcag cgtgccctgg gcgccctcga agatgacgag gcggtcgcgg tcgagcgcgt
    27181 cccacacgac ccgtgtcgtg tccgagatgt agggctcgag ccggtgcccg agccgcccgt
    27241 actcctcgac catcgtgtgc aggtcgagct ccggctcctt cgcgtacagg cgcagcgact
    27301 ggcgcttcgg ctccatcgcc gcgacgatct tctgccgcag gatcttctcg tcgagcaggt
    27361 cctgcacgcg gatgccgagg cgcagcgcct tgtccgcgta gcaggggccg atgccgcgcc
    27421 gggtggtacc gatctggagc ttgcccagcc gcgcctcgcc ggcgtggtcg agcagccggt
    27481 ggtagggcat gatcaggtgc gcgttcgcgg agatcttgag cccgccggtg tcgatgccgc
    27541 gatcgtgcag cccggcgatc tcgtcggtga tcacctgcgg gtcgaccacc acgccgttgc
    27601 cgatcaggca gtgcttgccg gggtagagga tccccgacgg gatcaggtgc agcttgaaca
    27661 cctcgtcgcc gcgcacgatc gtgtgcccgg cgttgttgcc gccctggtag cggatgatca
    27721 cgtccgcgcg ctcggcgagg aggtcggtga cctttccctt gccctcgtcg ccccactggg
    27781 cgccgaccaa gacgagaccc ggcatcgccc tcagcccctg gtgctcacgc ggcgtcggcc
    27841 tcgaacggca ccggcgcatc cgggtcgcgc ttcgtcatgc tcatgaactt cgggtagcgg
    27901 ttgaggaacg tcagcaccac ggtgccgacc gggccgttgc ggtgcttggc gatcaggatg
    27961 tccgcctcgc cgggacgctc gctctcctcc ttcttgtagt actcctcgcg gtagatgaac
    28021 atgacgaggt cggcgtcctg ctcgatctgc ccggactcgc ggaggtcgga gagcatcggc
    28081 tccggagggt tcctctgctc gaccgcgcgc gagagctgtg agaccgcgat caccgggatg
    28141 ctgagctcgc gcgcgaggat cttcagcccg cggctgatct ggccgatctg ctcgacgcgc
    28201 ccgaccgagg agtcctcggg ccgcatgagc tgcaggtagt ccacgacgac cagtcccagc
    28261 gggttgcgtg cgtggaggcg gcgcgccttg gcgcgcaggt cgaggatgct gaggtcgctc
    28321 gagtcgtcta tgtacagcgg cgcggcagcg agcttctcgg tcgcctgcat caccttcggc
    28381 cagcggtccg gccggacctt gcccttgcgc aggtcgtcgc cgttgagctt cgcctgcgag
    28441 gcgatgaagc gctgggccaa ctccgtctcg gacatctcca ggctgaacag cgcgaccggc
    28501 tgcccgtact tgaccgaggc gttctccgcg atgttcgtca cgagcgcgct cttgcccatc
    28561 gcggggcgtg ccgccagcac gatcaggttc cccggctgga agccaccggt caggttgtcc
    28621 aggtcgttga agccggagcg cgtacccgtg atcgcgatgc cctcgcgcga cacccgctcg
    28681 agcttctcga gctcgtcgtg caggacgtcc tcgatcgagc gcagctcccc gctgtgctcg
    28741 tcgtgcgcga tccggtagag cttcgcctcg gcctggtcga gcagctcctt cggctcgccg
    28801 gcgaagccat ggacgtcttc ctggatgccc cgcgtggtgt cgagcaagcg ccgcatgagg
    28861 gcgtggtcct tgacgatccg cgcgtactgc cggacgttgc cggccgaggg gacgaggttc
    28921 ggcagcgagt gcacgtaggc ggtaccgccc gcctcgtcga ggtcgcccgc ccggttgagc
    28981 tcctcgctga ccgtgagaac gtccaccggc tccgggtcgg ccttctcctt gagccggatc
    29041 atcgccgcga agatcaggcg gtggcgctgg cggtagaagt cctcggcgcg cagcttgacg
    29101 tcgatctgga tgccgtcgag cgcctgctcc gagagcagaa ttgcgcctag gaccgagacc
    29161 tctgcttcct ggttctgcgg agggaccttc cccgccgcat caggcgggtc cga
//
LOCUS       DASVPL010000106         9888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_208191, whole genome shotgun sequence.
ACCESSION   DASVPL010000106 DASVPL010000000
VERSION     DASVPL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208191"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VF517_05435"
     CDS             <1..1124
                     /locus_tag="VF517_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8052414.1"
                     /translation="LSLPMDDWEAEPQQYGDELHTRRVSGRFAPDPAVVATIAEKLEA
                     ASNPVLVVGGEADASGGWDAAVRLAEKQRLPVFAAALEGRAGFPEDHPNFQGVLPPAL
                     GAVGQFLEGRDLVVVAGAAVFKYYPNIPGDIVPAGTEVWQITSDPDEAARAQVGNAAI
                     GDAGAFLEALAERVGDSDRDAPPPRAAMPPPEETDPMPAGAAFDVLAGVLGGDEILVN
                     ESPSNALAFRARIKPSRPGSFMFGAGGGLGFGLSAAVGAQIGSPDRPVVAVLGEGSAQ
                     YTITALWTAVAYKVPLTILVLRNDEYAILKWFSDLEQVAGAPGLDLPGLDCVALAKAY
                     GMRADRVEGADALRDALADGIGASEPRLIEVRIAPGMSV"
     gene            1285..2133
                     /locus_tag="VF517_05440"
     CDS             1285..2133
                     /locus_tag="VF517_05440"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052415.1"
                     /translation="MASRAVLITGASTGIGRASAVRLAGAGWDVFAGVRKPEDGEAVR
                     SESPDRIQPLILDVTDAATIEAATNALSEALDGRGLAGLVNNAGITVQGPLEFLPLDD
                     LRKQLEVNVVGQVAVTQAVMPEIRRATGRIVNMGSVGGRVAHPILGPYHASKYAMEAL
                     TDSLRKELRQWGIHVIVVEPGSMATEIWDKGVRGAQEQLDRIGERGRELYGPTIEKIR
                     AVAAKTGERGGSPDIVAKVVERALTAKRPRTRYLVGADAFAQVALDTILPDRVFDAVE
                     ASYLRG"
     gene            complement(2153..2908)
                     /locus_tag="VF517_05445"
     CDS             complement(2153..2908)
                     /locus_tag="VF517_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8052416.1"
                     /translation="MDSTGPIRIAAAGDVHIREANCEQVSEAFAGLEGNCDAILVAGD
                     LTTHGEPEQAELFARAVKPLHVPVVAVLGNHDLHVDRGDEFRAVLAGEGIVVLERETT
                     VLELAGQEVGIVGLKGFVGGFAGSNLPDFGERQLRDVYATTSADVEALERGLNEVANC
                     PFRIVLLHYSPSTETLEGEPPGIWAFLGSDRLAAPIREHAPDLVLHGHAHAGTFEGRI
                     GDSPVYNVSVPVIGRDFWVFELSTPRRPTTPIH"
     gene            2932..3552
                     /locus_tag="VF517_05450"
     CDS             2932..3552
                     /locus_tag="VF517_05450"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEX8052417.1"
                     /translation="MPRSETMQAIAESMKKAAAALRQHDVPFLLAGSLASWARGGPET
                     SHDLDFVVKPEDAERALEALTEAGMKPEKPPEEWLYKAWDGDVLIDIIFSPSGLEPDD
                     DVMARGDTINVMSMELQVMAIEDVLVTKLNALTEHSLRYEGLLEMARALREQIDWRKV
                     RSRANDTPFARAFFVMVEGLGIAPDLPPRDPDGDDDQRVRVDVTTA"
     gene            complement(3559..4539)
                     /locus_tag="VF517_05455"
     CDS             complement(3559..4539)
                     /locus_tag="VF517_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052418.1"
                     /translation="MSLAPRRHLLIASIALGVSLAAPAAGTAGSYDFGSELSSPANAV
                     EAHGADTVYWDGASAAPDTGQLRYVTIRGGVLPGGGTGDFRFVALHPDGASVKVEQID
                     QRIRTLPTTTDPLRRVPNKDFNWNLCLHRGDHLGLWKVGHGNLQVFGDVPGSVTSWYE
                     NASGLARGDVFAGAASGDRELLMQVSITTGADAFSRCPGGYKDHVYRGLDVARARLSG
                     QALALRARCPRETYGGCFGRLAVRARIGGRTVTLGRARFALPSGRRFDPRIALAPAHA
                     ALIRRRGSLRADVVMTGHDDPSDIRNRRAPAPRPGRQSDTGTVRVTIAAG"
     gene            4637..5095
                     /locus_tag="VF517_05460"
     CDS             4637..5095
                     /locus_tag="VF517_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8052419.1"
                     /translation="MQRFYDAFARGDGDAMAACYAPGAHFSDPVFTDLRGEEPGAMWR
                     MLTGRAKDLEVRLEEHDAGDERGSARWLADYTFSTGRKVHNDVRAEFTFRDGLIAEHR
                     DSFSFYAWSRQALGPPGLLLGWTPIVRGKVQREARSGLDEFMAGGSGAAA"
     gene            complement(5121..5348)
                     /gene="secG"
                     /locus_tag="VF517_05465"
     CDS             complement(5121..5348)
                     /gene="secG"
                     /locus_tag="VF517_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX8052420.1"
                     /translation="METILLFVQIILSAIVVLLVLLHSGKDAGLSGAFGVGGGSGPFG
                     GGSLVERNLDRWTIFFAILFAVNIFILLKAF"
     gene            complement(5360..5650)
                     /locus_tag="VF517_05470"
     CDS             complement(5360..5650)
                     /locus_tag="VF517_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052421.1"
                     /translation="MEFALAFAVLAVVVALVVARPLRSPDEVETEDQRRAALEAAKEG
                     KYREIRDAELDREMGKLSGEDWRAVDRDLRAQAIAILHELDELDDGAAEERG"
     gene            complement(5655..7820)
                     /locus_tag="VF517_05475"
     CDS             complement(5655..7820)
                     /locus_tag="VF517_05475"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX8052422.1"
                     /translation="MTADIGSACLVVALLTALYAAGAALGGAIGGQRELVTSARRAFY
                     CVAGLVVLAVVLLQAAYVRTDLSFELVARNSSTDTPLFYRVTAMWSSQEGSLLLWALL
                     LSIYSSAVLFVTRRRMRDIVPYATAVLAGITAFFLSLMMVFGANPFSTLSTAPAQGAG
                     LEPLLRHPAMAIHPPMLYSGYVGFAIPFAFAIGALIVRRTDADWIRATRRFALIAWTF
                     LTCGVLLGALWSFSELGWGGYWAWDPVENASLMPWLLGTAFLHSVMVQEKRGMLKIWN
                     ATLIVGAFVMALIGTFLVRSGILDSIHAFGASTLGIPFLIFIGVVLVVSVTLIFGRRE
                     ELRSEARLDSLLSREAFFLLNNLVLVVMCLVVFWGTFFPLISEAITGDRRSLGPPFFN
                     RVTVPLALVLVLLSGIGPMLTWRKASSGALRRSFQWPVVASIAALAAMFVLTPAEESV
                     PSLLMFTFCALVVAVVVQELVRGTSARRTMTGEGPVAAFGSLVARNRRRYGGYLVHVG
                     VAIAFLGVAASSAFLEQRDVRMVAGQTTKVGEYEITYRGPTGKILEDDSSTGAPITLG
                     AVIDVRKGDETWTMRPQRNYYTANDGQGGAFGRFFMGESTSEVDLRWGVTRDLWTAVQ
                     PDLSVLMEPIRKANRQFADANEETQALIVAALADRYRRHAPPATFRFIDSPMVAWIWI
                     GGALGLLGAAWALWPAAEARRRRAASLAAARLGRELSRA"
     gene            complement(7842..8261)
                     /locus_tag="VF517_05480"
     CDS             complement(7842..8261)
                     /locus_tag="VF517_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX8052423.1"
                     /translation="MDPKRKRTIRLIVALGAAVLLAGALVYTSFSGSAEARKPSELTN
                     AQPGQAYKVTGKVVPGTIRKENTADGHRLFFRVRDRDGHASIPVVYEGVVPDPFRENR
                     EVIVAGEMKGGTFVGERDSLVTKCPSKFKNKPSGDTL"
     gene            complement(8322..8672)
                     /locus_tag="VF517_05485"
     CDS             complement(8322..8672)
                     /locus_tag="VF517_05485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8052424.1"
                     /translation="MESWDIRSLDVQPHQPKVLRTDDEARVITINLPAGEALQDHQVH
                     ERAYVFVADGAVEIEAGGGKETGSVGHLAHFDPNERHEVRATEDARIVLMLAPWTGEG
                     HSRERAGGGDPDQR"
     gene            complement(8733..9224)
                     /locus_tag="VF517_05490"
     CDS             complement(8733..9224)
                     /locus_tag="VF517_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8052425.1"
                     /translation="MTARNSFSFRREPQTVRSARRALDEFEGEFPQNRLYDASLCLSE
                     LVTNAVQHPGAGGDLVLTVAVDEARLRVEVADRGRGFDPGPPTEGDERGWGLFIVDSL
                     ADNWGVDPGEGTVVWFEIAREERAGEPGRAESEGGQEGGDSRADGLLRATALRLRSRL
                     AAP"
     gene            complement(9271..>9888)
                     /locus_tag="VF517_05495"
     CDS             complement(9271..>9888)
                     /locus_tag="VF517_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEX8052426.1"
                     /translation="RLTPTSERGDSAERDASAAKPAEPKEDHPVRAATNAEQERSTRV
                     QAAASTTVSIRDFSFAPRSVTVNTGDTVTWRNTGDEPHTATGSGFDTGLLKSGQSGSA
                     TFSQAGTFSYKCQPHPFMTGTVRVVASGSSGGGGGGSSSGGGTQGATNDGGAGGTGTA
                     GTGSTGAGAGSLPSTGLALGSVVLTGLAMIAMGTVLRRRAAWPSA"
BASE COUNT         1425 a         3546 c         3630 g         1287 t
ORIGIN      
        1 tcctgtcgct gccgatggac gactgggagg cggagccgca gcaatatggc gacgagctcc
       61 acacgcgccg cgtgagtggg cgcttcgcgc cggacccggc ggtggtggcg acgatcgcgg
      121 agaagctcga ggccgcctcg aaccccgtgc tggtcgtggg cggcgaggcc gacgcgagcg
      181 gtggctggga cgccgccgtg cggctcgccg agaagcagcg gctgccggtg ttcgccgccg
      241 cgctcgaggg ccgcgccggc ttcccggagg accatccgaa cttccagggc gtgctgccgc
      301 cggcgctcgg ggccgtcggc cagttcctcg agggccgcga cctcgtggtg gtcgccggcg
      361 ccgcggtgtt caagtactac ccgaacatcc cgggcgacat cgtcccagcg ggcacggagg
      421 tctggcagat cacgagcgac cccgacgagg cggcgcgggc gcaggtcggc aacgccgcga
      481 tcggggacgc gggcgcgttc ctcgaggcgc tggccgagcg ggtcggcgac tccgaccgcg
      541 acgctccgcc gccgcgcgcc gcgatgccgc ccccggagga gaccgacccg atgcccgccg
      601 gcgccgcctt cgacgtgctc gcgggagtgc tcggcgggga cgagatcctc gtgaacgagt
      661 cgccgtcgaa cgcgctggcg ttccgcgcgc gcatcaagcc gtcgcggccg gggtcgttca
      721 tgttcggcgc gggcggcggg ctcggcttcg ggctctcggc ggcggtgggc gcgcagatcg
      781 gctcgcccga ccggccggtg gtggccgtgc tcggcgaggg ctcggcgcag tacacgatca
      841 ccgcgctgtg gacggcggtg gcctacaagg tgccgctcac gatcctcgtc ctccgcaacg
      901 acgagtacgc gatcctgaag tggttctccg acctggagca ggtagcgggc gcccccgggc
      961 tcgacctgcc ggggctcgac tgcgtcgcgc tcgccaaggc gtacgggatg cgcgcggacc
     1021 gcgtcgaggg cgccgacgcc ctgcgcgacg cgctggccga cgggatcggc gcgagcgagc
     1081 cgcggctgat cgaggtccga atcgcacccg gaatgtcggt gtaggcgccc gcggcgaggc
     1141 cgcttttcgt cggcattact gacctaaggg tccatacgac ccaaaagtca ggaatgccga
     1201 ccaattccct cccgtcccgc ctccctgagt gatgccgttg aaccggcacc ccgcccgcgc
     1261 cgcgcgcgtg cgaccatccc ggccatggcc tcccgcgcgg tcctcatcac cggtgcctcg
     1321 accggcatcg gccgcgccag cgccgtgcgg ctggcgggcg cgggctggga cgtcttcgcc
     1381 ggggtccgca agccggagga cggcgaggcg gtgcggagcg agtcgcccga tcggatccag
     1441 ccgctgatcc tcgacgtgac ggacgccgcc acgatcgagg ccgcgaccaa cgcgctgagc
     1501 gaggcgctgg acggtcgcgg gctggccggg ctcgtgaaca atgccggcat cacggtccag
     1561 gggccgctcg agttcctgcc gctcgacgac ctgcgcaagc agctcgaggt gaacgtggtc
     1621 ggccaggtgg cggtcacgca ggcggtgatg cccgagatcc gccgcgccac gggccggatc
     1681 gtgaacatgg gctccgtggg cgggcgggtc gcgcacccga tcctcgggcc ctaccacgcg
     1741 tcgaagtacg cgatggaggc cctcaccgac tcgctgcgca aggagctgcg ccagtggggg
     1801 atccacgtga tcgtggtcga gccggggagc atggccaccg agatctggga caagggcgtg
     1861 cgcggggcgc aggagcagct cgaccggatc ggcgagcgcg gacgcgagct gtacggcccg
     1921 acgatcgaga agatccgcgc ggtggcggcc aagaccggcg agcgcggcgg gtcgccggac
     1981 atcgtggcga aggtggtgga gcgggccctg acggcgaagc gcccgcgcac gcgctacctc
     2041 gtgggcgccg acgccttcgc gcaggtcgcg ctcgacacga tcctgccgga ccgcgtgttc
     2101 gacgcggtcg aggcgagcta cctgcgcggt tagccgcctc ggccgatcgc ggtcagtgga
     2161 tcggcgtggt cgggcggcgc ggcgtcgaga gctcgaagac ccagaagtcg cgcccgatga
     2221 ccggcaccga gacgttgtag accggcgagt cgccgatccg cccctcgaat gtgcccgcgt
     2281 gggcgtggcc gtggaggacg aggtccggcg cgtgctcgcg gatcggcgcg gcgaggcggt
     2341 cgctgcccag gaacgcccag atgccgggcg gctcgccctc gagcgtctcg gtgctcggcg
     2401 agtagtgcag gagcactatc cggaacgggc agttcgccac ctcgttgagg ccgcgctcga
     2461 gcgcctcgac gtcggcgctc gtggtcgcgt acacgtcgcg cagctggcgc tcgccgaagt
     2521 ccggcaggtt cgagcccgcg aagccgccga cgaacccctt cagccccacg atcccgacct
     2581 cctggccggc cagctcgagc acggtcgtct cgcgctcgag cacgacgatc ccctcccccg
     2641 cgagcaccgc gcggaactcg tcgccgcgat ccacgtgcag gtcgtggttg ccgagcaccg
     2701 ccacgaccgg gacgtgcagc ggcttgaccg cgcgcgcgaa cagctcggcc tgctccggct
     2761 cgccgtgggt cgtcaggtcg ccggccacca ggatcgcgtc gcagttgccc tcgagtcctg
     2821 cgaacgcctc cgacacctgc tcgcagttcg cctcgcggat gtggacgtcg ccggccgcgg
     2881 cgatccgaat cggtcctgtt gagtccataa gtggttgggt atcaaagcga aatgccgcgc
     2941 agcgagacca tgcaggcgat cgccgagtcg atgaagaagg cggcggccgc cctccgccag
     3001 cacgacgtcc cgttcctcct ggcgggcagc ctcgcctcct gggcgcgcgg cggccccgag
     3061 acctcgcacg acctcgactt cgtggtgaag cccgaggacg ccgagcgcgc gctcgaggcg
     3121 ctcaccgagg cggggatgaa gccggagaag ccgcccgagg agtggctcta caaggcctgg
     3181 gacggtgacg tgctgatcga catcatcttc agcccgagcg gcctcgagcc cgacgacgac
     3241 gtgatggcgc gcggcgacac gatcaacgtg atgagcatgg agctccaggt gatggcgatc
     3301 gaggacgtgc tcgtgacgaa gctgaacgcg ctcaccgagc attccctgcg ctacgagggc
     3361 ctgctcgaga tggcccgcgc gcttcgcgag cagatcgact ggcggaaggt gcgctcgcga
     3421 gcgaacgaca cgcccttcgc gcgcgcgttc ttcgtgatgg tcgagggcct cgggatcgcc
     3481 ccggacctgc ccccgcggga ccccgacggc gacgacgacc agcgcgtgcg cgtggacgtg
     3541 accacggcct agggcggact agccggcggc gatcgtcacg cggacggtgc cggtgtccga
     3601 ctggcgcccc ggccgcggtg cgggtgcgcg ccggttgcgg atgtcgctcg ggtcgtcgtg
     3661 cccggtcatg acgacatccg cccgcagcga gccgcgccgg cggatcagcg ccgcgtgcgc
     3721 cggcgcgagc gcgatccgtg ggtcgaagcg ccgccccgac gggagtgcga agcgcgcgcg
     3781 gccgagcgtg acggtgcgcc cgccgatccg ggcgcgcacg gcgaggcgcc cgaagcagcc
     3841 gccgtaggtc tcgcgcgggc agcgcgcgcg tagggcgagc gcctggcccg agagccgcgc
     3901 acgagcgacg tcgaggccgc ggtagacgtg gtccttgtag ccgccgggac agcgcgagaa
     3961 ggcatcggcg ccggtcgtga tcgagacctg catcaggagc tcgcggtcgc cgctcgccgc
     4021 gccggcgaac acgtcgccgc gcgctagccc ggaggcgttc tcgtaccagc tcgtgaccga
     4081 gcccggcacg tcgccgaaca cctggaggtt gccgtgcccg accttccaga ggccgaggtg
     4141 atcgcccctg tgcaggcaga ggttccagtt gaagtccttg ttcggcacgc gccgcagcgg
     4201 gtccgtggtg gtcgggagcg tccggatccg ctggtcgatc tgctcgacct tcaccgacgc
     4261 gccgtccgga tgcagcgcga cgaagcggaa gtccccggtc ccgccgccgg gcagcacccc
     4321 gccgcggatc gtcacgtagc ggagctgccc ggtgtcgggc gccgcgctcg cgccatccca
     4381 gtagaccgtg tcggcgccgt gtgcctccac cgcgttggcc ggcgaggaga gctcgctgcc
     4441 gaagtcgtag gagcccgcgg tgccggcggc gggcgcggcg agtgataccc cgagcgcgat
     4501 cgaggcgatc agcaggtgac ggcgcggagc gagcgacatc gagcggtcgg actaggaacc
     4561 cgccgtcgcc cccgaagtag tggtcccgcg ccacaaccat tagcctgccg ctatcgcgca
     4621 cccgaacgac gagctgatcc agcgcttcta cgacgccttc gcgcgcggcg acggcgacgc
     4681 catggccgcc tgctacgcgc ccggcgcgca cttctccgac ccagtcttca ccgacctgcg
     4741 cggcgaggag cccggcgcga tgtggcggat gctcaccggc cgcgcgaagg acctcgaggt
     4801 gcggctcgag gagcacgatg ccggcgacga gcgcggcagc gcgcgctggc tcgccgacta
     4861 cacgttcagc accgggcgca aggtccacaa cgacgtgcgc gcggagttca ccttccgcga
     4921 cggcctgatc gccgaacacc gcgactcgtt ctcgttctac gcctggtcgc gccaggcgct
     4981 ggggccgccc gggctgctgc tcggctggac cccgatcgtg cgcggcaagg tgcagcgcga
     5041 ggcccgctcg gggctcgacg agttcatggc cggcggttcc ggcgccgccg cgtagccgcc
     5101 gcgcctgaga cgcagccggc ctagaacgcc ttcagcagga tgaagatgtt gaccgcgaag
     5161 aggatcgcga agaagatcgt ccagcgatcg aggttgcgct cgaccagcga gccgccgccg
     5221 aacggaccgc tgccgccgcc gacgccgaag gcgcctgaga ggcccgcgtc cttgcccgag
     5281 tgcagcagca ccagcaggac gacgatggcc gacaggatga tctggacgaa gagaaggatc
     5341 gtctccacgg gctcgtaggt tagccgcgct cctccgcggc gccgtcgtcg agctcgtcga
     5401 gttcgtgcag gatcgcgatg gcctgcgcgc gcaggtcgcg atcgaccgcg cgccagtcct
     5461 cgccggagag cttgcccatc tcgcgatcga gctccgcgtc gcggatctcg cggtacttgc
     5521 cctccttggc ggcctcgagc gccgcgcgcc gctggtcctc ggtctcgacc tcgtccgggc
     5581 tgcgcagcgg ccgggccacg acgagcgcga ccacgaccgc gagcacggcg aacgcgaggg
     5641 cgaactccaa ggggctatgc ccgcgagagc tcgcggccga gccgcgcggc ggcaaggctg
     5701 gccgcgcggc gccggcgcgc ctcggcggcg ggccacagcg cccaggccgc gcccagcagc
     5761 ccgagggcgc cgccgatcca gatccacgcg accatcggcg agtcgatgaa gcgaaacgtc
     5821 gcgggcgggg catggcggcg gtagcggtcg gccagcgcgg ccacgatcag cgcctgggtc
     5881 tcttcgttgg cgtcggcgaa ctggcggttg gccttccgga tcggctccat caacacgctc
     5941 aggtcgggct gcacggcggt ccagaggtcg cgcgtgacgc cccagcgcag gtccacctcg
     6001 ctggtcgact cgcccatgaa gaagcgcccg aacgcgccgc cctggccgtc gttcgcggtg
     6061 tagtagttgc gctgcgggcg catcgtccac gtctcgtcgc ccttgcgcac gtcgatcacg
     6121 gcgccgagcg tgatcggggc cccggtggag gagtcgtcct cgaggatctt gcccgtaggc
     6181 cctcgatagg tgatctcgta ctcgcccacc ttcgtcgtct gcccggccac catccgtacg
     6241 tcgcgctgct cgaggaacgc cgacgacgcg gccaccccga ggaacgcgat cgccacgccg
     6301 acgtgcacga ggtagccgcc gtagcggcgc cggttgcgcg ccacgagcga gccgaacgcg
     6361 gcgaccggcc cctcgccggt catcgtgcgc cgcgccgacg tcccgcgcac gagctcctgg
     6421 acgaccaccg cgaccacgag cgcgcagaac gtgaacatga gcagcgacgg gacgctctcc
     6481 tcggccggcg tgagcacgaa catcgccgcg agcgcggcga tcgacgccac caccggccac
     6541 tggaacgagc gccgcagcgc gccgctgctc gccttgcgcc aggtgagcat cggcccgatc
     6601 cccgacagca gcacgagcac cagcgcgagc ggcacggtca cgcggttgaa gaacggcggc
     6661 ccaaggctgc gccggtcgcc ggtgatcgcc tcggagatca gcgggaagaa cgtgccccag
     6721 aagaccacca ggcacatgac cacgagcacg aggttgttga ggaggaagaa cgcctcgcgc
     6781 gagagcagcg agtcgagccg cgcctcgctg cgcagctcct cccgtcggcc gaagatcagc
     6841 gtcaccgaga cgaccagcac cacgccgatg aagatcagga acgggatgcc gagcgtggac
     6901 gctccgaacg cgtggatcga gtcgaggatc cccgaccgca ccaggaacgt gccgatcagc
     6961 gccatcacga acgccccgac gatcagcgtc gcgttccaga tcttcagcat cccgcgcttc
     7021 tcctgcacca tcaccgagtg caggaacgcc gtgccgagca gccacggcat gagcgacgca
     7081 ttctcgaccg ggtcccaggc ccagtagccg ccccagccga gctcggagaa cgaccagagc
     7141 gcgccgagca gcactccgca ggtcaggaag gtccacgcga tcagcgcgaa gcggcgggtg
     7201 gcgcggatcc agtcggcgtc ggtgcggcgc acgatcagcg ctccgatggc gaaggcaaac
     7261 gggatcgcga agccgacgta gcccgagtag agcatcggcg ggtggatcgc catcgccggg
     7321 tggcgcagca gcggctcgag cccggcgccc tgcgcggggg cggtggaaag cgtgctgaac
     7381 gggttcgcgc cgaagaccat catcagcgag aggaagaagg cggtgatgcc cgcgagcacg
     7441 gcggtggcgt acgggacgat gtcgcgcatc cggcggcggg tcacgaacag caccgcgctc
     7501 gagtagatcg agagcagcag cgcccacaga agcagcgagc cctcctggct cgaccacatc
     7561 gcggtgaccc tgtagaagag cggcgtgtcg gtcgacgagt tgcgcgctac gagctcgaag
     7621 ctgaggtccg tgcgcacgta ggccgcctgg agcagcacga ccgcgagcac gaccaggccg
     7681 gcgacgcagt agaaggcgcg ccgcgccgag gtgaccagct cgcgctgccc gccgatcgcg
     7741 ccgccgaggg ccgcgccggc ggcgtagagc gccgtcagca gcgcgacgac gaggcaggcg
     7801 gagccgatgt cggcggtcat gcggcctccg ctcgcatggc gctagagcgt gtcgccgctt
     7861 ggcttgttct tgaacttcga ggggcacttc gtgacgagcg agtcgcgctc gccgacgaac
     7921 gtgccgccct tcatctcgcc cgccacgatc acctcgcggt tctcacggaa cgggtcgggc
     7981 acgacgccct cgtagaccac cgggatcgag gcgtgaccgt cgcggtcgcg gacccggaag
     8041 aagagccggt ggccgtcggc ggtgttctcc ttgcggatcg tgcccgggac caccttgccg
     8101 gtgaccttgt acgcctggcc cggctgcgca ttcgtcagct cggagggctt gcgcgcctcg
     8161 gcggacccgc tgaagctcgt gtagacgagc gcgcccgcga gcagcacggc ggcgccgagc
     8221 gccacgatca ggcggatggt ccgcttgcgc ttggggtcca tgctccgacg attatcgcag
     8281 ggccggcacg ccccgcccgc gccccgcgag cgcggccgcg cctagcgctg gtcggggtcg
     8341 ccgccgcccg cgcgctcgcg cgagtggccc tcgccagtcc acggcgccag catcagcacg
     8401 atccgcgcgt cctcggtcgc ccggacctcg tgccgctcgt tgggatcgaa gtgcgccagg
     8461 tggccgaccg agccggtctc cttgccgccc ccggcctcga tctccaccgc gccgtcggcg
     8521 acgaacacgt aggcgcgctc gtggacctgg tggtcctgga gggcctcgcc ggcgggcagg
     8581 ttgatcgtga tcacgcgcgc ctcgtcgtcg gtcctcagga ccttcggctg gtggggctgc
     8641 acgtcgaggg agcggatgtc ccagctttcc aagtcgtctc ctgtccttcg cgggtggggc
     8701 cggcgggcat catacgcgcg ccgcgcgcgg actcagggcg cggcaaggcg gctgcgcagc
     8761 cgcagcgcgg tggcgcgcag caggccatcc gcccgcgagt cgccgccctc ctgcccgccc
     8821 tcgctctcgg cccgccccgg ctcgccggcc cgttcctcgc gcgcgatctc gaaccagacc
     8881 acggttccct cgccgggatc caccccccag ttgtcggcca ggctgtccac gatgaacagt
     8941 ccccagccgc gctcgtcgcc ctcggtgggc gggcccgggt cgaagccgcg gccccggtcg
     9001 gccacctcca cgcgcaggcg cgcctcgtcg accgcgacgg tgagcaccag gtcaccgccc
     9061 gcgccggggt gctggacggc gttggtcacg agctcgctga ggcacagcga cgcgtcgtag
     9121 aggcggttct gcgggaactc gccctcgaac tcgtccagtg cgcggcgcgc ggagcggacg
     9181 gtctgcggct ctctcctgaa gctgaagctg ttgcgcgcgg tcacgggcgg gtcgatcctc
     9241 ggggcccccc gcccggcgcg gatgatacgc ctacgcggag ggccaggcgg cgcggcggcg
     9301 cagaacggtg cccatcgcga tcatcgcgag gcccgtcagg accacggaac ctagcgccag
     9361 accggtgctg gggagcgagc cggcgcccgc gccggtgctg cccgtgccgg cggtgccggt
     9421 gccgccggcg ccaccatcgt tcgtggcgcc ctgggtgccg ccgccggagg aggagccccc
     9481 gccgccaccg ccggaggagc cggaggccac gacgcgcacc gtgccggtca tgaacgggtg
     9541 cggctggcac ttgtaggaga acgtgccggc ctggctgaag gtcgccgagc cgctctggcc
     9601 gctcttcagg agcccggtgt cgaagcccga gcccgtggcg gtgtggggct cgtcgccggt
     9661 gttgcgccag gtgacggtgt cgccggtgtt cacggtcacc gaccgcggcg cgaacgagaa
     9721 gtcgcggatc gagacggtgg ttgacgcggc ggcctggacg cgggtcgagc gctcctgctc
     9781 cgcgttcgtg gccgcccgga ccgggtggtc ctccttcggc tccgccggct tggcggcgga
     9841 ggcgtcgcgc tcggcggaat ccccgcgctc gctcgtcgga gtcaaccg
//
LOCUS       DASVPL010000107         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_287459, whole genome shotgun sequence.
ACCESSION   DASVPL010000107 DASVPL010000000
VERSION     DASVPL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287459"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF517_05500"
     CDS             <1..703
                     /locus_tag="VF517_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8052427.1"
                     /translation="RLAPAGSTAARGLAAATATDAAARQLAMSRMVQAHDRPALLTPD
                     FRDGAAEASIAAAVSQHVDRPLSVLAETLYLDSRLALVDLMLLYFDKMSMAASLEVRV
                     PFLDHDVVAFCSALPDSRRGRLLRRKELLKRASRGLVDDEIIDKRKRAFFRGALDPWL
                     RAHGDGLFTDLLLDERALGRGQFQPDAVRGLVASAGVAGTKASQKLFCLLLLEKWQRL
                     WVDPDGRGRRTAPRL"
     gene            700..>1698
                     /locus_tag="VF517_05505"
     CDS             700..>1698
                     /locus_tag="VF517_05505"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEX8052428.1"
                     /translation="MRTPLAPLLCAVAMVVVAWALFVPPFQVPDESGHFSYVQSLAEN
                     GERPVGGPAFGGPPRFSSEERWALSLSGAQRSFGDTGFDPAWDEASEAGWDRVDGELR
                     PDRGDIAAAGAQARHSPLYYGYETAPYAASGGDLFDRLYLMRLWSGALMLVTTLGAWL
                     LVGELTGRDRLLQLAGASCVGLQPMATFVSAGVNPDAALFASSSLSLWLGVRVLRRGP
                     TRASVAGLLAATALAALAKAAGLALPATLIFDHPTPAAVAKFVREQVEGAARPAAAPA
                     RAKPSTDEPIAIVGISCRYPGGVRSAEDLWRLVASGGDAIAPFPEDRGWDVERLYDP"
BASE COUNT          178 a          636 c          640 g          244 t
ORIGIN      
        1 gcggctcgcc ccggcgggat cgacggcggc ccgggggctc gccgcggcga ctgccacgga
       61 cgcggcggcc cggcagctcg cgatgagccg catggtccag gcgcacgatc gccccgcgct
      121 gctcaccccc gacttccgcg acggcgcggc cgaggcgagt atcgccgccg cggtcagcca
      181 gcacgtggac cggcctctgt ccgtgctcgc cgagacgctg tacctcgata gccggctggc
      241 gctcgtggac ctgatgctcc tctacttcga caagatgtcg atggccgcgt cgctcgaggt
      301 gcgcgtcccg ttcctcgacc acgacgtcgt cgcgttctgc tccgcgctgc cggactcgcg
      361 ccgggggcgg ctgctgcgcc gcaaggaact cctcaagcgc gcgagtcgcg ggctggtgga
      421 cgacgagatc atcgacaagc gaaagcgcgc gttcttccgc ggggccctcg acccgtggct
      481 gcgcgcccac ggcgacggcc tgttcaccga cctcctgctc gacgagcgcg cgctggggcg
      541 cgggcagttc caaccggacg ccgtccgcgg cctcgtcgcg tcggccgggg tggccgggac
      601 caaggcgagc cagaagctct tctgcctcct gctgctcgag aagtggcaac ggctgtgggt
      661 cgacccggac ggccgcgggc gccgcaccgc gccgcgcctg tgaggactcc gctcgcgccg
      721 ctgctctgtg ccgtggcgat ggtcgtcgtc gcgtgggcgc tgttcgtgcc gccgttccag
      781 gtgcccgacg agagcggcca cttctcgtac gtccagtcgc tcgccgagaa cggcgagcgg
      841 ccggtgggcg ggcccgcgtt cggcggcccg ccgcgcttct cgagcgagga gcgctgggcg
      901 ctcagcctct cgggcgcgca gcgcagcttc ggcgatacgg ggttcgaccc ggcttgggac
      961 gaggcgtcgg aggcgggctg ggaccgcgtc gacggcgagc tgcgccccga ccgcggcgac
     1021 atcgcggcgg cgggcgcgca ggcgcggcac tcgccgctct actacggcta cgagacggcg
     1081 ccgtacgccg cgtcgggcgg cgacctgttc gaccggctct acctcatgcg cctgtggtcg
     1141 ggcgcgctga tgctcgtcac cacgctcggc gcgtggctgc tcgtcggcga gctgaccggc
     1201 cgcgatcgcc tgctccagct cgcgggcgcc tcctgcgtgg gcctccagcc gatggccacg
     1261 ttcgtgtccg ccggcgtgaa tccggacgcg gcgctgttcg cgtcgtcgtc gctctcgctc
     1321 tggctcgggg tgcgggtgct gcggcgcggg ccgacgcgcg cgtcggtggc cggtctgctc
     1381 gcggccaccg cgctggcggc gctcgcgaag gcggcggggc tcgcgctgcc cgcgacgctc
     1441 atcttcgacc acccgacgcc ggccgcggtc gcgaagttcg tccgcgagca ggtcgagggc
     1501 gccgcccgtc ccgccgccgc tcccgcgcgc gcgaagccga gcacggacga gccgatcgcg
     1561 atcgtgggca tcagctgccg ctacccgggc ggcgtacggt cggccgagga cctgtggcgc
     1621 ctggttgcct ccggaggcga cgcgatcgcg ccgttcccgg aggatcgcgg ctgggacgtc
     1681 gagcgcctct acgacccg
//
LOCUS       DASVPL010000108        13286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_367129, whole genome shotgun sequence.
ACCESSION   DASVPL010000108 DASVPL010000000
VERSION     DASVPL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13286
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367129"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5817
                     /locus_tag="VF517_05510"
     CDS             <1..5817
                     /locus_tag="VF517_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8052429.1"
                     /translation="QVRIIAQVSTAVARGDLTQKITVEAKGEVATLAETINEMTDTLA
                     VFADQVTGVAREVGTEGKLGGQADVPNVAGTWKDLTENVNLMASNLTDQVRNISQVAT
                     AVARGDLTQKITVEAKGEVATLADTMNEMTDTLAIFAEQVTGVAREVGTEGKLGGQAD
                     VPNVAGTWKDLTDSVNLMAGNLTSQVRNIADVTTAVANGDLSQKITVDVKGEILDLKR
                     TINTMVDQLSSFAEQVTLVAREVGTEGKLGGQAEVQGVSGTWRDLTENVNVMASNLTD
                     QVRNISQVATAVARGDLTQKITVEAKGEVATLAETINEMTDTLAVFADQVTGVAREVG
                     TEGKLGGQADVPNVAGTWKDLTDSVNFMASNLTDQVRNIAQVATAVARGDLTQKITVE
                     AKGEVATLAETINEMTDTLAVFAEQVTGVAREVGTEGKLGGQAEVEGVAGTWKDLTDS
                     VNLMASNLTDQVRNIAQVTTAVANGDLSQKITVDVRGEIRELKDTINTMVDQLSSFAD
                     EVTRVAREVGTEGELGGQAEVKGVSGTWRDLTENVNLMASNLTDQVRNIAEVATAVAE
                     GDLSQKIAVEAKGEVAALADTLNTMVDRLRSFAAEVTRVAREVGTEGKLGGQAEVEDV
                     SGTWRGLTENVNLMASNLTDQVRNIAEVTTAVAEGDLSKKITVEARGEILELKDTINT
                     MVDQLRSFAAEVTRVAREVGTEGKLGGQAEVEDVSGTWRGLTENVNMMASNLTDQVRN
                     IAEVTTAVANGDLSKKITVDAKGEILELKDTINTMVDQLIAFAAEVTRVAREVGTEGK
                     LGGQAEVEGVSGTWRGLTENVNYLASNLTDQVRNIADVTTAVANGDLSKKITVDARGE
                     ILELKDTINTMVDQLSSFADEVTRVAREVGTEGILGGQAQVEGVSGTWRGLTENVNQL
                     ASNLTTQVRAIAEVSTAVTQGDLTRAVTVDASGEVADLKDNINQMIANLRETTSRNEA
                     QDWLNSNLARFSGLMQGQRDLETVSRLIMSELTPTVSGQHGAFFLAEADAAEAADAER
                     EAELKLVASYGYKKRKNVSNRFKPGEGIVGQAALERKGITITEPPKDYIKVTSGLGEA
                     APRNIVVLPVPFEEQVLGVIELASVQEFTEVHLQFLEQLMETIGVVLSTIIANTRTEQ
                     LLAQSQSLTQELQSQSEELQTQQEELRRSNEELEEQAKSLKASEELLQQQQEELQQTN
                     EELQEKASLLQQQNRDIEIKNREIELARRSLEEKAEQLALSSRYKSEFLANMSHELRT
                     PLNSLLILSELLAKNEGENLDEKQVEFATTIHNSGADLLSLINDILDLSKVEAGKMDV
                     HPRHVPIAEVAGQVDRGFRAVAEQRGLEFEIELDENLPTAIETDEQRLQQVLKNLLSN
                     AFKFTESGTVTLRIAPAPEDIAFAGEVLNRATSVIGFSVVDTGIGIAEDKLRLIFEAF
                     QQADGTTSRRYGGTGLGLSISREIARLLGGEIRVASTEGEGSTFTLYLPAGWSVTEFM
                     PAAGDGVEPAPILAALGAGPNGGGVAEAAEPARDAVAALPDGLVDDRDSLLPGDRVLL
                     VIDGDRPTAEHALAAGRERGFKVLLGMRGEAGLALAHEHKPDAVLLGLQLPGGDGAAV
                     LDHLKRHPDTRHVPVHAMSDTDRRQEVLRAGAVGYLERPVAAEALATSLDELAAFMER
                     RVRNLLVVEDDDTERKAVAELIGGGDDVEITAVGTSEEALAELARTSFDCIVLDLKLP
                     DSTGFQLLEKIKKSDDWCDIPVIVHTGKSLTRREETRLKKYAETIVVKDARSPERLLD
                     ETSLFLHRVEARMPEDKRRMLEQLHTADAVFAGKRVLIVDDDVRNVFALTSVFEARGM
                     DVLFAENGRDGLETLEQNPDVDLVLMDIMMPEMDGYETTRAVRERPEFKNLPIVALTA
                     KAMKGDREKSIAAGASDYITKPVDVDQLLSLMRVWLYR"
     gene            5829..6674
                     /locus_tag="VF517_05515"
     CDS             5829..6674
                     /locus_tag="VF517_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX8052430.1"
                     /translation="MAVTDLARPSPDLERIETALLLEGIYQHYGFDFRGYAWASLKRR
                     LWRRAQAEGVRSLSALQERVLHDPPAMERLLLDLSINVTTMFRDPTFFAAFREKVVPL
                     LKTYPFVRIWNAGCSTGEETYSLAIILREEGLYDRARIYATDINDVVLEHARSATYPL
                     DRMRDYTENYIRAGGTESFSAYYEADGEGARFDPALTEQIVFAQHNLVSDGAFNQFNV
                     IVCRNVMIYFGKPLQERVHDLFHESLETFGVLALGHKESIRFTTHEPHYEELDANERL
                     YRKVR"
     gene            6675..7253
                     /locus_tag="VF517_05520"
     CDS             6675..7253
                     /locus_tag="VF517_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX8052431.1"
                     /translation="MGYELIAIGASWGGLHAVGEVLAGLPEDFGPAIVVAQHRSRDSV
                     KGMLTTALGTRTALPVCEAGDKDPIKRSRVHLAPPDYHLLVEDGHFELSLDPPVSHSR
                     PSIDVLFESVADAYGTRAIGVLLTGASSDGAAGLLRLREMGGLTIVQDPEEAERPEMP
                     AAALELDAAEQVCPLASVAPFLLDACGAGRTA"
     gene            7250..9787
                     /locus_tag="VF517_05525"
     CDS             7250..9787
                     /locus_tag="VF517_05525"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX8052432.1"
                     /translation="MSAAPGVRGETRTSVLIVDDKPQNLIALDAVLRPLELDVVSAES
                     GEEALKLLLDRDYAAILLDVQMPGMDGFETAEFIKRRERTAHIPIIFLTAIDKERQKV
                     FRGYQVGAVDYVFKPYDPDVLRSKVATFVELYRKTAALRASEERFRAAFANAPIGIGL
                     MGPDGNWIQANAALCELVGHPQHEFLSKPLWELAVPDDRKRDREEMRGALDARVGAFE
                     SERRLRHADGSTVHVLVSASLASAQEDGAPTWVVQLVDLTERKRAERERAEREHERAA
                     RAEAEAVADTIQRIQRVSDVALAHLELGDLVRALVELLREIVVVDAAAILLMEANDSR
                     LRLVGGAGLSNLPERDLLIPIGEGFAGRVAVAGTAEAGAPVPRDGGLETVLAAAGMRS
                     ALVVPMSVEGRVSGAIAVARRSAAPFPEGDHRLLQLIADRAALAIEHARLYQRELGIV
                     EVLQRSLLPERLPRLPGVDLAARYMPGGVGADVGGDWYDAIPLAGGRIGMALGDVVGH
                     GIGAAALMGQLRNALRAYALDGHSPSEVVSRLDRLVQRLEHGRMATLLFMVFEPDLGT
                     VHFSSAGHLPPLVVEPDGSARYLDGGRTPPLGVMPLAEHLDATAEIPAGSTLVLYSDG
                     LVEERGVGLDRGLATLSRATVEGPDDPEALCDHIIESLFEERSPNDDVAVLVLRTVAL
                     HGNRLELELTADPDAQRTMRRTVGRWLDRAGVVPEQTNEVQVACHEVCSNAIEHGHRF
                     GDDVVRIEAEIDGDVLAMTVVDTGTWREERVTDRGRGLSMARAFMDTVDVETGPEGTR
                     VSMTRRLTFVARDGEGSAGDGSAGNGAKKKKPAARRKRAASGPARSR"
     gene            complement(9814..12276)
                     /locus_tag="VF517_05530"
     CDS             complement(9814..12276)
                     /locus_tag="VF517_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX8052433.1"
                     /translation="MPRTSRISVPLLALCAAFAAPAPAPAATETVSAGALEAEVQSDP
                     WRVELRGPGATLSQRLPLGYRTAAGWSRATRATDLRRDGDALLATVATDTGGAIAVRI
                     RPAGDGTIALEATPEAGADAVGMGFDAPAGERFFGFGSRSDAVDHRGREVENYVADGP
                     TRPEDRNYPRAVVPPWSHGERDDSTYYPVPWLLSSRGYGVLVDRDETSRFRLASEDEP
                     GGWSVEVDAPALALRVFAGPTPADALRRFTAATGRQPPPPAPWTFGPWFQTGQPNRVE
                     LAQEAEMIRVQRDADAPVSAAETQMHFLPCGAHRDDPAYNAARTEQFHAAGLAQLVYF
                     NPSLCTSYQPVWSEAAAQGLLIRERSGQPHTYPAFVGGSGPGGFTTEPLSQFDFTAPG
                     TGPYYERLLREATDAGHDGWMEDFGEGAPPTGMAADGTTGSALHNRYPTDYHCAAQRA
                     VERIGRPVVRHQRSGWTGAARCAVNVWGGDPTTVWDYDGLASGVKQALGIGMSGVSRF
                     GTDIGGYNTYGPGENLTPELLVRWIQFGAVSGVMRTKYSGLAVPSYRRPQVFDPEILP
                     VWRRYTKLHTQLYPYLLAADAAYRSSGLPLMRHMALAAPGDARAIAADDQFLFGPDLL
                     AAPVTAPGATTRRLYAPAGRWIDWWRSVRFKQGDGSFRPGVARVLKGRREHTLPAPRD
                     ELPLLARAGSVLPMLSADVDTLAPYGGNGVVRLDDRDDRLTLLAFPRGSWRGAFYERE
                     KLRSTELRGGGWRLGISGARKRTYSVEASFAALERRFSPCSVFVGERRLGKGEWSWRP
                     RSRTFRARFSAKRAALTVRRCR"
     gene            complement(12380..>13286)
                     /locus_tag="VF517_05535"
     CDS             complement(12380..>13286)
                     /locus_tag="VF517_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8052434.1"
                     /translation="AFGLAESLAVLVAARFVQGLGGAASWTGALGWLVAAGPPERRGE
                     LIGTAFGAAVAGSLLGPVLGAIADGTTPELAFGAVAALAVGLTAATLATPKPVTAAGA
                     SGGFRLALRQRPVLAGMAMIVLVGLFFGVLEVLVPLRLDELGGGTVLIAGTFLAGAGL
                     QALASPVAGRHVDRRGPLPIVRAALLAAVALALVVPALGAVAPLVVLAVAAGPLVGML
                     WVPGMTALSDGADTVGLDQAFAFALVNLTWAGAQTAGAGGGGALADSAGDWASYGAVA
                     ALMALALAVTLRARGEPEPARTAST"
BASE COUNT         2105 a         4613 c         4908 g         1660 t
ORIGIN      
        1 caggtgcgca tcatcgccca ggtctcgacc gccgtcgcgc gcggggacct gactcagaag
       61 atcacggtcg aggccaaggg cgaggtggcc acgcttgccg agaccatcaa cgagatgacc
      121 gacacgctcg cagtgttcgc cgaccaggtg accggcgtgg cgcgcgaggt gggcaccgag
      181 ggcaagctcg gcgggcaggc cgacgtgccg aacgtggccg gcacctggaa ggacctcacc
      241 gagaacgtga acctgatggc gtcgaacctg accgaccagg tgcgcaacat ctcgcaggtc
      301 gcgacggcgg tcgcgcgcgg cgacctgact cagaagatca cggtcgaggc caagggcgag
      361 gtggccacgc tcgccgacac gatgaacgag atgaccgaca cgctcgcgat cttcgccgag
      421 caggtcaccg gcgtggcgcg cgaggtcggc accgagggca agctcggcgg ccaggccgac
      481 gtgccgaacg tcgcgggcac gtggaaggac ctcaccgaca gcgtgaacct gatggccggc
      541 aacctcacga gccaggtgcg caacatcgcc gacgtgacga cggccgtcgc gaacggcgac
      601 ctctcgcaga agatcacggt ggacgtgaag ggcgagatcc tcgacctgaa gcggacgatc
      661 aacacgatgg tcgaccagct ctcgtcgttc gccgagcagg tcacgctggt ggcgcgcgag
      721 gtcggcaccg agggcaagct cggcggccag gccgaggtgc agggcgtctc cggcacgtgg
      781 cgtgacctca ccgagaacgt gaacgtgatg gcgtcgaacc tgaccgacca ggtgcgcaac
      841 atctcgcagg tcgcgaccgc cgtagcgcgc ggcgacctga ctcagaagat cacggtcgag
      901 gccaagggcg aggtggccac gctcgccgag acgatcaacg agatgaccga cacgctcgcg
      961 gtgttcgccg accaggtgac cggcgtggcc cgcgaggtcg gcacggaggg caagctcggc
     1021 ggccaggccg acgtgccgaa cgtggccggc acgtggaagg acctcaccga ctccgtgaac
     1081 ttcatggcgt cgaacctcac cgaccaggtg cgcaacatcg cccaggtcgc gaccgccgta
     1141 gcgcgcggcg acctgacgca gaagatcacc gtcgaggcca agggcgaggt ggccactctc
     1201 gccgagacga tcaacgagat gaccgacacc ctggccgtgt tcgccgagca ggtcaccggc
     1261 gtggcgcgcg aggtgggcac cgagggcaag ctcggcggtc aggcggaggt cgagggcgtc
     1321 gccgggacct ggaaggacct gaccgacagc gtgaacctga tggcctcgaa cctgaccgac
     1381 caggtccgca acatcgctca ggtcacgacc gcggtggcga acggcgacct gtcgcagaag
     1441 atcaccgtgg acgtgcgcgg cgagatccgc gagctgaagg acacgatcaa caccatggtc
     1501 gaccagctct cgtcgttcgc cgacgaggtc acgcgcgtgg cgcgcgaggt cgggaccgag
     1561 ggcgagctgg gcgggcaggc cgaggtgaag ggcgtctccg gcacgtggcg cgacctgacc
     1621 gagaacgtga acctgatggc gtcgaacctg accgatcagg tccgcaacat cgccgaggtc
     1681 gcgaccgcgg tcgccgaggg cgacctgtcg cagaagatcg ccgtcgaggc caagggcgag
     1741 gtggcggccc tggccgacac gctgaacacg atggtcgacc gcctgcgctc gttcgcggcg
     1801 gaggtcacgc gcgtggcgcg cgaggtgggc accgagggca agctcggcgg ccaggccgag
     1861 gtcgaggacg tgtccggcac ctggcgcggg ctcaccgaga acgtgaacct gatggcctcg
     1921 aacctgaccg atcaggtccg caacatcgcg gaggtcacca cggcggtcgc cgagggcgac
     1981 ctgtcgaaga agatcaccgt cgaggcacgc ggcgagatcc tcgagctgaa ggacacgatc
     2041 aacacgatgg tggatcagct ccgctcgttc gcggcggagg tcacgcgcgt ggcgcgcgag
     2101 gtcggcaccg agggcaagct cggcggccag gccgaggtgg aggatgtcag cggcacgtgg
     2161 cgcgggctca ccgagaacgt gaacatgatg gcctcgaacc tgaccgacca ggtgcgcaac
     2221 atcgccgagg tgacgacggc ggtcgccaac ggcgacctct cgaagaagat cacggtcgac
     2281 gccaagggcg agatcctcga gctgaaggac accatcaaca cgatggtcga ccagctcatc
     2341 gcgttcgccg ccgaggtcac gcgcgtcgcg cgcgaggtcg gcaccgaggg caagctgggc
     2401 ggccaggccg aggtcgaggg cgtgtcgggc acctggcgcg gactgaccga gaacgtgaac
     2461 tacctcgcgt ccaacctcac cgaccaggtc cgcaacatcg ccgacgtgac gacggccgtc
     2521 gccaacggtg acctctccaa gaagatcacg gtcgacgcgc gcggcgagat cctcgagctg
     2581 aaggacacga tcaacacgat ggtcgaccag ctgagctcgt tcgccgacga ggtcacgcga
     2641 gtcgcccgcg aggtcggcac cgagggcatc ctcggcggcc aggctcaggt cgagggcgtg
     2701 tctggcacct ggcgcgggct caccgagaac gtgaaccagc tcgcgtcgaa cctcacgacc
     2761 caggtgcgcg cgatcgccga ggtctccacc gcggtgaccc agggcgacct cacgcgcgcg
     2821 gtcacggtcg acgccagcgg cgaggtggcg gacctcaagg acaacatcaa ccagatgatc
     2881 gccaacctgc gcgagaccac ctcgcgcaac gaggcgcagg actggctgaa ctcgaacctc
     2941 gcccgcttca gcggcctcat gcagggccag cgcgacctcg agacggtgtc gcgcctgatc
     3001 atgtcggagc tgacgccgac ggtctccggc cagcacggcg cgttcttcct ggccgaggcc
     3061 gacgcagccg aggcggccga cgccgagcgc gaggccgagc tgaagctcgt cgcgagctac
     3121 ggctacaaga agcgcaagaa cgtctcgaac cgcttcaagc cgggcgaggg catcgtcggc
     3181 caggcggcgc tcgagcgcaa gggcatcacg atcaccgagc ccccgaagga ctacatcaag
     3241 gtcacctcgg gcctaggcga ggcggccccg cgcaacatcg tcgtgctccc ggtcccgttc
     3301 gaggagcagg tgctcggcgt gatcgagctg gcatcggtgc aggagttcac cgaggtgcac
     3361 ctccagttcc tcgagcagct catggagacg atcggcgtgg tgctctcgac gatcatcgcc
     3421 aacacccgta ccgagcagct gctggcccag tcgcagtcgc tgacgcagga gctccagagc
     3481 cagtccgagg agctccagac ccagcaggag gagctgcgcc gctcgaacga ggagctcgag
     3541 gagcaggcga agtcgctcaa ggcgtccgag gagctgctcc agcagcagca ggaggagctc
     3601 cagcagacca acgaggagct gcaggagaag gcgtcgctgc tccagcagca gaaccgcgac
     3661 atcgagatca agaaccgcga gatcgagctg gcccgccgca gcctggagga gaaggccgag
     3721 cagctcgcgc tcagctcgcg ctacaagtcg gagttcctgg cgaacatgtc gcacgagctg
     3781 cgcacgccgc tcaacagcct tctgatcctg tccgagctgc tcgccaagaa cgagggtgag
     3841 aacctcgacg agaagcaggt cgagttcgcc acgacgatcc acaactccgg cgcggacctg
     3901 ctctcgctga tcaacgacat cctcgacctg tcgaaggtcg aggccggcaa gatggacgtg
     3961 cacccgcggc acgtgccgat cgccgaggtc gcgggccagg tggaccgcgg gttccgtgcg
     4021 gtcgccgagc agcgcggcct cgagttcgag atcgagctgg acgagaacct cccgaccgcg
     4081 atcgagaccg acgagcagcg cctccagcag gtgctgaaga acctgctctc gaacgcgttc
     4141 aagttcaccg agagcgggac cgtgacgctg cgcatcgcgc ccgcgcccga ggacatcgcg
     4201 ttcgccggtg aggtgctcaa ccgcgccacg tcggtgatcg gcttctcggt ggtggacacg
     4261 ggcatcggga tcgccgagga caagctgcgg ctgatcttcg aggcgttcca gcaggccgac
     4321 ggcaccacca gccgccgcta cggcgggacc gggctcgggc tctcgatcag ccgcgagatc
     4381 gcgcgcctgc tcggcggcga gatccgcgtg gccagcaccg agggggaggg gagcacgttc
     4441 acgctctacc tacccgcggg ctggagcgtg acggagttca tgccggccgc cggcgacggg
     4501 gtcgagcccg caccgatcct cgcggcgctc ggggccgggc cgaacggcgg cggggttgcc
     4561 gaggcggcgg agcccgcccg cgacgcggtc gcggccctgc cggacgggct cgtggacgac
     4621 cgcgactcgc tgctgccggg cgatcgcgtg ctgctcgtga tcgacggcga ccggccgacg
     4681 gccgagcacg cgctggcggc cgggcgcgag cgcgggttca aggtcctgct cggcatgcgc
     4741 ggcgaggccg ggctggcgct cgcccacgag cacaagccgg acgcggtgct cctcgggctc
     4801 cagctcccgg gcggcgacgg cgccgccgtg ctcgaccacc tcaagcgcca ccccgacacg
     4861 cgccacgttc cggtgcacgc gatgtcggac accgaccggc gccaggaggt gctgcgagcg
     4921 ggggcggtcg gctacctgga gcgcccggtc gccgccgagg ccctggcgac ctcgctcgac
     4981 gagctggccg cgttcatgga gcgccgcgtg cgcaacctgc tggtggtcga ggacgacgac
     5041 accgagcgca aggcggtggc cgagctgatc ggcggcggcg acgatgtcga gatcacggcg
     5101 gtggggacga gcgaggaggc gctggccgag ctcgcgcgca cgtcgttcga ctgcatcgtg
     5161 ctcgacctga agctccccga ctcgaccggc ttccagctcc tcgagaagat caagaagagc
     5221 gacgactggt gcgacatccc cgtgatcgtg cacaccggca agtcgctgac ccggcgcgag
     5281 gagacgcgcc tcaagaagta cgccgagacg atcgtcgtga aggacgcgcg ctcgccggag
     5341 cggctgctgg acgagacgtc gctgttcctg caccgcgtcg aggcgcggat gcccgaggac
     5401 aagcggcgga tgctcgagca gctccatacc gcggacgcgg tgttcgccgg caagagggtg
     5461 ctgatcgtgg acgacgacgt gcgcaacgtg ttcgcgctca ccagcgtgtt cgaggcgcgc
     5521 gggatggacg tgctattcgc cgagaacggt cgtgacgggc tcgagacgct cgagcagaac
     5581 cccgacgtgg acctggtgct gatggacatc atgatgcccg agatggatgg gtacgagacc
     5641 acgcgcgcgg tccgcgagcg ccccgagttc aagaacctcc cgatcgtcgc gctcaccgcg
     5701 aaggcgatga agggcgaccg cgagaagagc atcgcggcgg gggcctcgga ctacatcacc
     5761 aagcctgtcg acgtcgatca gctcctgtcg ctgatgcgcg tctggctgta tcgatagccg
     5821 gcgctcccgt ggccgtgacg gacctggcac gtccgagccc cgacctcgag cggatcgaga
     5881 ccgccctcct gctcgagggc atctaccagc actacgggtt cgacttccgc gggtacgcgt
     5941 gggcgtcgct caagcggcgc ctgtggcggc gcgcccaggc ggagggagtg cgatcgctgt
     6001 cggcgctcca ggagcgcgtg ctgcacgacc cgcccgcgat ggagcggctg ctgctcgacc
     6061 tctcgatcaa cgtcaccacg atgttccgcg acccgacgtt cttcgcggcg ttccgcgaga
     6121 aggtcgtgcc gctcctgaag acctatccct tcgtgcggat ctggaacgcg ggctgctcga
     6181 ccggcgagga gacgtactcg ctcgcgatca tcctgcgcga ggagggcctc tacgaccgcg
     6241 cgcgcatcta cgcgaccgac atcaacgacg tggtgctcga gcacgcccgc agcgctacct
     6301 atccgctcga ccggatgcgc gactacacgg agaactacat ccgggcgggc gggaccgagt
     6361 cgttctcggc ctactacgag gccgacggcg agggcgcgcg cttcgatccg gcgctgaccg
     6421 agcagatcgt gttcgctcag cacaacctcg tctccgacgg cgcgttcaac cagttcaacg
     6481 tgatcgtgtg cagaaacgtc atgatctact tcggcaagcc cctacaggag cgggtgcacg
     6541 acctgttcca cgagagcctc gagaccttcg gtgtgctcgc cctaggacac aaggagtcga
     6601 tccgattcac cacgcacgag ccgcattacg aggagctcga cgcgaacgag aggctctacc
     6661 ggaaggtgcg ctgagtgggg tacgagctga tcgcgatcgg ggcgtcgtgg ggcgggctgc
     6721 acgcggtcgg cgaggtgctg gccgggctgc ccgaggactt cggaccggcc atcgtcgtgg
     6781 cccagcatcg ctcgcgtgac tcggtcaagg gcatgctgac gaccgcgctc gggaccagga
     6841 ccgcgctgcc ggtctgcgag gccggcgaca aggacccgat aaagcggagt cgagtgcatc
     6901 tcgcgccgcc ggactaccac ctgctcgtgg aggacggtca cttcgagctg tcgctggacc
     6961 cgccggtgag ccacagccgc ccctcgatcg acgtgctgtt cgagtcggtg gcggacgcgt
     7021 acgggacgcg ggcgatcggg gtgttgctca cgggcgcgag ctcggacggc gccgccgggc
     7081 tgctgcgcct gcgcgagatg ggcgggctca cgatcgtcca ggacccggag gaggccgagc
     7141 gccccgagat gcccgctgcc gcgctggagc tggacgccgc cgagcaggtg tgcccgctgg
     7201 catcggtcgc gccgttcctg ctcgacgcgt gcggggcggg ccggaccgca tgagcgcggc
     7261 gcccggcgtg cgtggcgaga cgcgcaccag cgtgctgatc gtggacgaca agccgcagaa
     7321 cctgatcgcg ctcgacgcgg tgctgcggcc gctcgagctc gacgtcgtgt ccgccgagtc
     7381 gggcgaggag gcgctgaagc tgctgctcga ccgcgactac gcggcgatcc tgctcgatgt
     7441 ccagatgccc gggatggacg gcttcgagac ggccgagttc atcaagcgcc gcgagcgcac
     7501 cgctcacatc ccgatcatct tcctgaccgc catcgacaag gagcgccaga aggtgttccg
     7561 cggctaccag gtgggggcgg tcgactacgt cttcaagccc tacgacccgg acgtgctgcg
     7621 ctcgaaggtg gccacgttcg tggagctgta ccgcaagacc gcggcgctgc gcgcgagcga
     7681 ggagcgtttc cgcgcggcgt tcgcgaacgc gccgatcggc atcgggctga tgggaccgga
     7741 cggcaactgg atccaggcca acgccgcgct ctgcgagctg gtcgggcatc cgcagcacga
     7801 gttcctgtcg aagccgctgt gggagctggc cgtgcccgac gaccgcaagc gcgaccgcga
     7861 ggagatgcgc ggggcgctcg acgcccgcgt gggcgcgttc gagagcgagc ggcggctgcg
     7921 gcacgctgat ggcagcacgg tgcacgtgct cgtgagcgcg tccctggcca gcgctcagga
     7981 ggacggtgcg ccgacctggg tggtgcagct cgtggacctc accgagcgca agcgcgccga
     8041 gcgcgagcgg gccgagcgcg agcacgagcg cgccgcgcgt gcggaggccg aggcggtggc
     8101 cgacacgatc cagcgcatcc agcgcgtgag cgacgtcgcg ctcgcgcacc tcgagctcgg
     8161 cgacctggtg cgggccctgg tcgagctgct gcgcgagatc gtggtggtcg acgccgccgc
     8221 gatcctgctc atggaggcca acgactcgcg gctgcggctg gtgggcggag ccggtctgag
     8281 caacctgccc gagcgcgacc tgctgatccc gatcggcgag ggcttcgcgg gccgcgtggc
     8341 ggtcgccggc acggccgagg cgggggcgcc ggtgccccgc gacggcggcc tcgagacggt
     8401 gctcgccgcg gcggggatgc gctcggcgct ggtggtgccg atgtcggtcg agggccgcgt
     8461 gagcggggcg atcgcggtcg cacggcgcag cgcggcgccg ttcccggagg gcgatcatcg
     8521 cctgctccag ctcatcgccg accgcgcggc gctggcgatc gagcacgcgc gcctctacca
     8581 gcgcgagctg gggatcgtcg aggtgctcca gcgcagcctc ctgccggagc ggctgccgcg
     8641 cctacccggc gtggacctgg cggcgcgcta catgcccggc ggcgtcggcg ccgacgtggg
     8701 cggcgactgg tacgacgcga tcccgctggc cggtgggcgc atcggcatgg cgctcggcga
     8761 cgtggtgggg cacggcatcg gggccgccgc gctgatgggt cagctccgca acgccctgcg
     8821 tgcgtacgcg ctcgacgggc actcgccgtc ggaggtggtc tcgcggctcg accggctggt
     8881 ccagcggctc gagcacgggc ggatggccac gctgctcttc atggtcttcg agcccgacct
     8941 gggcacggtg cacttctcga gcgcggggca ccttccgccg ctcgtggtgg agccggacgg
     9001 ctcggcccgc tacctcgacg ggggccgcac gccgccgctc ggggtcatgc cgctcgccga
     9061 gcacctcgac gcgacggcgg agataccggc tggctcgacg ctcgtgctct acagcgacgg
     9121 tctggtggag gagcgcggcg ttggactcga ccgcgggctc gcgacgctct cgcgcgcgac
     9181 cgtggagggg cccgacgacc ccgaggcgct ctgcgaccac atcatcgagt cgctcttcga
     9241 ggagcgctcg cccaacgacg acgtggcggt gctcgtcctg cgcacggtgg cgctccacgg
     9301 caaccgcctg gagctcgagc tgacggcgga cccggacgcc cagcggacca tgcgccggac
     9361 ggtggggcgc tggctcgacc gggccggggt tgtgccggag cagaccaacg aggtgcaggt
     9421 cgcctgccac gaggtctgct cgaacgccat cgagcacggc caccgcttcg gggacgacgt
     9481 ggtgcgcatc gaggccgaga tcgacggcga cgtgctggcg atgacggttg tcgacacggg
     9541 cacctggcgc gaggagcgcg tgaccgaccg cggccgcggg ctgagcatgg cccgggcgtt
     9601 catggacacg gtcgacgtgg agaccggtcc cgaggggacg cgggtgagca tgacgcggcg
     9661 gctgaccttc gtcgcgcggg acggggaggg ctccgcgggc gacggctccg ccggcaacgg
     9721 cgcgaagaag aagaagccgg ccgcgcggcg caagcgcgcg gcctctgggc cggcgcgctc
     9781 gcgctgagcg tgctcgcgcg ctcgcgctga gtcctagcgg cagcgccgca ccgtcaacgc
     9841 ggccctcttc gcgctgaaac gcgcgcggaa ggtccgcgag cgtgggcgcc aggaccactc
     9901 tcccttcccc agccgccgtt ctcccacgaa aaccgagcac ggagagaacc gtcgttcgag
     9961 cgccgcgaaa gaagcctcga ccgagtaggt ccgcttgcgc gcccccgaga tgcccaggcg
    10021 ccagccgccg ccgcgcagct cggtggagcg cagcttctcg cgctcgtaga acgcgccgcg
    10081 ccagctcccg cgggggaacg cgagcagcgt gaggcggtcg tcgcggtcgt ccagtcggac
    10141 cactccgttg ccgccgtacg gagcgagcgt gtccacgtcc gcgctcagca tcggcagcac
    10201 gctcccggcg cgcgccagca gcggcagctc gtcgcgcggg gcgggcagcg tgtgctccct
    10261 tcgtcccttc agcacgcgcg cgacgccggg gcggaacgag ccgtcgccct gcttgaagcg
    10321 cacgctgcgc caccagtcga tccaccgccc cgcgggcgcg tagagcctgc gcgtggtcgc
    10381 gcccggcgcg gtcacgggag cggccaggag gtcgggtccg aacaggaact ggtcgtcggc
    10441 ggcgatcgcg cgcgcgtcgc cgggggcggc gagggccatg tgccgcatga gcggcagccc
    10501 gctcgaccgg tacgcggcgt cggcggcgag caggtagggg tagagctgtg tgtggagctt
    10561 cgtgtagcgg cgccagaccg gcaggatctc cgggtcgaac acctgcggcc gccggtacga
    10621 gggcaccgcc agccccgagt acttcgtgcg catcacgccc gagaccgcgc cgaactggat
    10681 ccagcgcacg agcagctccg gcgtgaggtt ctcccccggt ccgtaggtgt tgtagccgcc
    10741 gatgtcggtg ccgaaccggc tcactcccga catgccgatc ccgagcgcct gcttcacgcc
    10801 cgaggcgagg ccgtcgtagt cccagacggt ggtcgggtcg ccgccccaga cgttcaccgc
    10861 gcagcgcgcc gcgccggtcc agcccgagcg ctggtggcgc acgaccggcc gcccgattcg
    10921 ctcgaccgcc cgctgcgcgg cgcagtggta gtcggtgggg tagcggttgt ggagcgccga
    10981 gccggtcgtg ccgtccgcgg ccatgccggt cggcggcgcg ccctcgccga agtcctccat
    11041 ccagccgtcg tgcccggcgt cggtggcctc gcggaggagc cgctcgtagt aggggccggt
    11101 gcccggagcg gtgaagtcga actgcgacag cggctcggtg gtgaagccgc ccggcccgct
    11161 gccgccgacg aacgccgggt aggtgtgcgg ctggcccgag cgctcgcgga tcagcaggcc
    11221 ctgggccgcg gcctcgctcc agaccggctg gtaggacgtg cacagcgacg ggttgaagta
    11281 cacgagctga gcgagcccgg ccgcgtggaa ctgctcggtg cgggcggcgt tgtaggcggg
    11341 gtcgtcgcga tgcgcgccgc aggggaggaa gtgcatctgg gtctcggcgg ccgagacggg
    11401 cgcgtcggcg tcgcgctgga ctcggatcat ctcggcctcc tgagccagct cgacgcggtt
    11461 cggctgcccg gtctggaacc acggcccgaa ggtccagggc gcgggcggcg gtggctggcg
    11521 gccggtcgcg gcggtgaagc gcctgagagc gtcggcgggg gtggggccgg cgaacacgcg
    11581 cagtgcgagc gcgggcgcgt cgacctccac gctccagccg cccggctcgt cctccgaggc
    11641 gagccggaag cggctggtct cgtcgcggtc gaccagcacg ccgtagccgc ggctcgagag
    11701 cagccacggg acggggtagt aggtcgagtc gtcgcgttcg ccgtgcgacc acggcggcac
    11761 gaccgcgcgc gggtaattgc ggtcctccgg gcgcgtgggg ccgtcggcga cgtagttctc
    11821 gacctcgcga ccgcggtggt cgacggcatc ggagcgcgag ccgaacccga agaagcgctc
    11881 gccggcgggg gcgtcgaagc ccatgccgac tgcgtcggct ccggcctccg gcgtggcctc
    11941 gagcgcgatc gtcccgtcgc cggcggggcg gatgcgcacg gcgatcgcgc cgccggtgtc
    12001 ggtcgcgacg gttgcgagga gcgcgtcgcc gtcgcgacgt agatcggtgg cacgggtggc
    12061 cctggaccac cccgcggcgg tccggtagcc gagcggcagg cgctggctga gggtggcgcc
    12121 ggggccgcgg agctccacgc gccacgggtc tgactgcacc tccgcctcga gcgccccggc
    12181 gctgaccgtc tccgtggcgg ccggcgccgg cgcgggtgcc gcgaaggcag cacatagagc
    12241 gagcagcggc accgagatgc gggatgtgcg gggcactcgc ggagcctacg tccgccccgc
    12301 cggccgcggc ctcacacgag gtgtcccgac ttaatggcgc ataccgccag aaaagccggg
    12361 cacctcgatc ccacctcccc taagtgctcg ccgtgcgcgc cggctccggc tccccgcgcg
    12421 cccgcagcgt gaccgccagc gccagcgcca tcagcgccgc cacggccccg taggacgccc
    12481 agtcgccggc ggagtccgcc agcgcgccgc caccgcccgc gcccgccgtc tgcgcgccgg
    12541 cccaggtcag gttcacgagg gcgaacgcga acgcctggtc gaggccgacg gtgtcggcgc
    12601 cgtcggagag cgccgtcatc ccgggcaccc agagcatccc cacgagcgga ccggccgcca
    12661 ccgcgaggac caccagcggc gcgaccgcac cgagcgccgg cacgaccagc gcgagcgcca
    12721 ccgccgcgag cagcgcggcg cgcactatcg gcagcgggcc gcgccggtcc acgtggcgtc
    12781 ccgcgacggg gctcgcgagc gcctggagcc cggcgccggc caggaacgtg ccggcgatca
    12841 gcacggtccc gccgccgagc tcgtcgagcc gcagcggaac cagcacctcg agcacgccga
    12901 agaagagccc gacgagcacg atcatcgcca tgccggcgag caccggccgc tggcgcaggg
    12961 cgagccggaa gccgccgctc gcgcccgcgg ccgtcaccgg cttcggcgtc gccagcgtcg
    13021 ccgcggtcag ccctaccgcg agcgccgcca ccgcaccgaa ggcgagctcg ggcgtggtcc
    13081 cgtcggcgat cgcgcccaac acggggccga gcagcgagcc cgccacggcc gcgccgaacg
    13141 ccgtgccgat cagctcgccg cggcgctccg gcggcccggc cgcgaccagc cagccgagcg
    13201 cgccggtcca ggacgccgct ccgccgagcc cctgcacgaa ccgagcagcc accagcaccg
    13261 ccaggctctc ggcgagcccg aaggcg
//
LOCUS       DASVPL010000109         9024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_326417, whole genome shotgun sequence.
ACCESSION   DASVPL010000109 DASVPL010000000
VERSION     DASVPL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326417"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /gene="uvrC"
                     /locus_tag="VF517_05540"
     CDS             <1..1296
                     /gene="uvrC"
                     /locus_tag="VF517_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX8052435.1"
                     /translation="EYRRSIDAVVGFLSGRYRQIERDLEHLMQEAAAEQEFEQAAAYR
                     NRLKAVRSLLERQRISNSAVGTLDAIAVAAEGTDANAQVFQIRDGVLADRQSFYLDNQ
                     AARPEGEVTEEFVLQYYATSPAIPPQIMVAGDVAIDALELALSEQRGAAVEVRHAERG
                     DKRRIFELAQRNARLALDQDRLRSEHRRVQRIEALELLQAALGMDVIPMRIECFDISN
                     LGETHTVASMVVFEGAAPKKSDYRRFGIRDVSQDDFAAMNEVLSRRMAQYVAHRERSP
                     HERSYDASFAAVPALIVIDGGKGQLSSGLAALQPFRDSGVTVVSLAKRVEEIFLPGRG
                     DPLLLPENSPALHLLQRVRDEAHRFAIEFHRGRRDKAMTRSVLDDVPGIGPARKRLLL
                     QHFGTPERFLQASREELEAVPGVPGKVAREVYEYVHKIR"
     gene            1271..2200
                     /gene="rapZ"
                     /locus_tag="VF517_05545"
     CDS             1271..2200
                     /gene="rapZ"
                     /locus_tag="VF517_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX8052436.1"
                     /translation="MSTSTRSGSDGAGHGLRGIEDFVLITGFSGAGKSQAMAAFEDAG
                     YFCVDNLPPEMISSLAELFAHEGSKVERAAVVCDVRGGAYFEGFARVLDELESRGLRH
                     RVLFLDASPDVLLHRFKETRRRHPLANGGQVTAAIQQERGLLEPLRGRADVAIDTTDL
                     SASRLRKIVVEKMLPQGTRGKLAVTILTFGFKHGAPGEADLMFDVRFLPNPHYEEELR
                     DLTGRDRPVIDYVESSEGIDEFYAKVMPLLDYLLPAYAREGKAHLTIGIGCTGGRHRS
                     VAIGDHLAREFAGRGDYLLDVVHRDVDKPPRRA"
     gene            2283..3287
                     /gene="gap"
                     /locus_tag="VF517_05550"
     CDS             2283..3287
                     /gene="gap"
                     /locus_tag="VF517_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX8052437.1"
                     /translation="MAVRVGINGFGRIGRNVWRAAHARGADIEWVGVNDLTDAKTLAH
                     LLKYDSILGPLDVDVSATDSGIVIDGKELAVFSERDPAALPWSDVGADVVIESTGLFT
                     DRENAQKHIDAGAKKVIISAPAKGPDATVALGVNFDSAYDRDNHHVISNASCTTNCLA
                     PVAKVLHDAVGIEHGVMTTIHAYTADQRLQDLPHKDLRRARAAAINLIPTSTGAAKAI
                     GLVVPELEGKLNGISVRAPLPTGSIVDLVFTASRETSVEEINAAVRERADTGPLEGIL
                     QYTEDPIVSSDIVSSPYSSIFDAPLTMVIEGKLVKVFAWYDNEWGYSNRVVELAQRVL
                     "
     gene            3294..4484
                     /locus_tag="VF517_05555"
     CDS             3294..4484
                     /locus_tag="VF517_05555"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX8052438.1"
                     /translation="MAGFGKKTLRDMQIGAGEAVLVRVDFNVPLANGEVADETRIRAA
                     LPAIEQLRDRGARLVLCSHLGRPKLREPATSLEPVSRRLGELLGTRVHQAPEVVGPEV
                     RRGAARLDPGEILVLENTRWEAGETKNDPELARELAELADHYVDDAFGAAHRAHASTV
                     GVTEHLPSAAGPLLEREVTTLTEIIEAPQRPLVVVLGGAKVSDKAALIDRFLDIADAL
                     LIGGAMCFSFFRAQGKDTGDSLVEEEGVELAREALVKAETSDCRLLLPTDLVLGDRFD
                     AGAEVRETAEVSVSKGWMGLDIGRESREVYADEIAKAGTVFWNGPMGAFEMATFAGGT
                     RRVAEAVAQAQGRTVVGGGDSAAALVSFGLADSVTHLSTGGGAALELLEGKPLPGVEA
                     LDDA"
     gene            4477..5238
                     /gene="tpiA"
                     /locus_tag="VF517_05560"
     CDS             4477..5238
                     /gene="tpiA"
                     /locus_tag="VF517_05560"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX8052439.1"
                     /translation="MPDRTPYVAGNWKMYKSVAETEEYIGRLLGRLPVGNNVTVGLCV
                     PFTALAKAVEKAEGSGVKVLAQTMHQAREGAFTGEVSAGMLAELGVDGVVLGHSERRR
                     DCGETDRALQEKVPAALEAGLVPILCVGESEDEFEAGETQRKLRHQVQEGLEKVPEDR
                     LADVVIAYEPIWAIGTGRNATPEQAEEAIGFIRALVGDRSKEAAQATRILYGGSVKSS
                     NAAEIMAQADVDGALVGGASLDPAEFAKIVEAAAP"
     gene            5262..6704
                     /gene="gpmI"
                     /locus_tag="VF517_05565"
     CDS             5262..6704
                     /gene="gpmI"
                     /locus_tag="VF517_05565"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX8052440.1"
                     /translation="MLDGWGLAPPGPGNAVELADTPVFDELWRSHPRTQLTAGGRAVG
                     LPEGQMGNSEVGHLNLGAGSVVRQDLTRIDDAVADGSFAQNEVLLDAMRTERLHLLGL
                     VSDGGVHASMDHLRALIELAAAQGVAEVLVHAFTDGRDTLPDSGAGYVEQVEEWLAAA
                     GGRIATVSGRYYAMDRDHRWERTDLALDAIVHARSEQPPAESGVAAVRAAYERGETDE
                     FVKPTVVGAGGRVRDGDSLVFFNFRPDRARQLSTRLADRRLTTLTEYQEDYTFPVAFP
                     PERPATTLAATLAERGIGQLHVAETEKYAHVTYFFNGGEEDEYKGEVRCLVDSPRDVP
                     TYDHKPEMSARAAADAFTGRWRQGGFGFGIINFANPDMVGHTGSIPAAVEAIETVDAC
                     LADVVAAVHESGGACIVTADHGNADNMLEPDGSPNTAHSLNPVPLVVTTAVPGLRDGG
                     VLADVAPTVLDLLGQEQPDAMTGRTLILPN"
     gene            6812..7255
                     /locus_tag="VF517_05570"
     CDS             6812..7255
                     /locus_tag="VF517_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX8052441.1"
                     /translation="MKPEPFSSDFNRLFSTLFDENRVQRWVPPMDLVEADDHFLLKAD
                     LPGLGEEDVAIEIRDNTLTISGERKSEYEKRERGWYRVERATGSFSRALSLPDGIDPD
                     AVKASFDKGVLEVWIPKPEARKPRRIAINAHANGNGNGAQPVIEG"
     gene            7287..8498
                     /gene="tgt"
                     /locus_tag="VF517_05575"
     CDS             7287..8498
                     /gene="tgt"
                     /locus_tag="VF517_05575"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX8052442.1"
                     /translation="MPDLPLEIGARSGSARAGVLRCAHGEVRTPAFVPLASTATVRSL
                     HASEVAELGYDMVLGNTFHLFLEPGHELIERMGGLHEFMAWRGPIVTDSGGFQVFSMG
                     HGSVAEEIKRRRGDRQSRILSIEEEGVRFRSYLDGSERFMGPETSMEIQAALGSDVVL
                     AFDECTPFHVDRDYTARAAERTHRWLDRCISWRREHAPEGGLFMGIVQGGVHQDLRAQ
                     SAERVASSEVDGVAVGGSLGQEKAQMHEVVEWSLAPVPDAKPRHLLGIGDVDDILAAV
                     GAGIDTFDCATPTRLARHGTALVSDPEARWRLDLTKARWRESREPIAPDCPCPACREH
                     TRGYLHHLSRTSELTGARLITLHNLTFMKALMDGIRTAIESGTYAEHAAAVLGGASPY
                     GAVPGRTAPYG"
     gene            complement(8489..>9024)
                     /locus_tag="VF517_05580"
     CDS             complement(8489..>9024)
                     /locus_tag="VF517_05580"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX8052443.1"
                     /translation="GAPPQGWNPQGAPPQGWGPQGGPPQGWQAAGPPGQPGQDPTEVV
                     GKRIGAGIIDFFLMVVLFFIVGIALGEGESGDGNASVTLEGGSALVYFALLLGYYIVT
                     EAVWNATLGKRALGLVVRSADGSKPSTGQIVGRNLLRIVDGFFFYLVGLITIVATKRK
                     QRLGDLAAGTIVVPRQP"
BASE COUNT         1338 a         3213 c         3254 g         1219 t
ORIGIN      
        1 gagtaccgcc gctcgatcga cgcggtcgtc ggcttcctgt cgggtcgcta ccgccagatc
       61 gagcgcgacc tcgagcacct gatgcaggag gcggctgccg agcaggagtt cgagcaggcg
      121 gccgcctacc gcaaccgcct aaaggcggtg cgctcgctgc tcgagcgcca gcggatctcg
      181 aactcggccg tcggcacgct cgacgcgatc gccgtggccg ccgagggcac cgacgcgaac
      241 gcgcaggtgt tccagatccg cgacggcgtg ctggccgacc gccagagctt ctacctcgac
      301 aaccaggccg cgcgcccgga gggcgaggtc accgaggagt tcgtgctcca gtactacgcg
      361 acctcgcccg cgatcccgcc gcagatcatg gtggccggcg acgtggcgat cgatgctctc
      421 gagctggcgc tgtccgagca gcgcggcgcg gccgtcgagg tccgtcacgc ggagcgcggc
      481 gacaagcgcc ggatcttcga gctggcccag cgcaacgcgc ggctcgcgct cgaccaggac
      541 cggctgcgct cggagcaccg gcgcgtgcag cggatcgagg cgctcgagtt gctccaggcg
      601 gcgctcggca tggacgtgat cccgatgcgg atcgagtgct tcgacatctc gaacctcggc
      661 gagacccaca cggtcgcctc gatggtcgtg ttcgagggcg ccgcgccgaa gaagtccgac
      721 taccgccgct tcgggatccg tgacgtctcg caggacgact tcgccgcgat gaacgaggtc
      781 ctgtcgcgcc ggatggcgca gtacgtcgcg caccgcgagc gttcgccgca cgagcgctcc
      841 tacgacgcga gcttcgccgc cgtgcccgcg ctgatcgtga tcgacggcgg caaggggcag
      901 ctctcctccg ggctcgccgc cctacagccg ttccgcgact cgggcgtgac cgtggtcagc
      961 ctggccaagc gcgtcgagga gatcttcctg cccgggcgcg gcgacccgct gctcctgccg
     1021 gagaactcgc cggcgctcca cctgctccag cgcgtgcgcg acgaggcgca ccgcttcgcg
     1081 atcgagttcc accgcgggcg ccgcgacaag gcgatgacgc gctcggtgct cgacgacgtg
     1141 ccggggatcg gtccggcccg taagcgactc ttgctgcagc acttcggcac cccggagcgt
     1201 ttcctccagg cgagccgcga ggagctcgag gcggtccccg gcgttccggg gaaggtcgcg
     1261 cgcgaggtct atgagtacgt ccacaagatc aggtagcgac ggcgcgggcc acgggctccg
     1321 cgggatcgag gacttcgtgc tgatcacggg cttctcgggc gcgggaaagt cgcaggcgat
     1381 ggccgccttc gaggacgcgg ggtacttctg cgtcgacaac ctaccgccgg agatgatcag
     1441 ctcgctcgcg gagctgttcg cgcacgaggg cagcaaggtc gagcgcgcgg cggttgtttg
     1501 cgacgtgcgc ggcggcgcgt acttcgaggg cttcgcgcgc gtgctcgacg agctcgagtc
     1561 acgcggcctg cgccaccggg tcctcttcct cgacgcctcg cccgacgtgc tgctgcaccg
     1621 tttcaaggag acccggcgcc ggcacccgct ggcgaacggg ggccaggtga ccgccgccat
     1681 ccagcaggag cgcggcctgc tcgagccgct gcgcgggcgg gccgacgtcg cgatcgacac
     1741 caccgacctc tcggcctcgc gcctgcgcaa gatcgtggtg gagaagatgc tcccgcaggg
     1801 cacgcgcggc aagctcgcgg tcaccatcct caccttcggc ttcaagcacg gcgcgccggg
     1861 cgaggccgac ctgatgttcg acgtgcgctt cctcccgaac cctcactacg aggaggagct
     1921 gcgcgacctg accggccgcg accggccggt gatcgactac gtggagagct ccgaggggat
     1981 cgacgagttc tacgcgaagg tcatgccgct gctcgactac ctgctccccg cgtacgcgcg
     2041 cgagggcaag gcgcacctga cgatcgggat cggctgcacg ggcggtcgcc accgctcggt
     2101 cgcgatcggc gatcacctgg cgcgcgagtt cgcgggccgc ggcgattacc tcctcgacgt
     2161 ggtgcaccgc gacgtggaca agccgccacg gcgcgcgtag cccgtcccgg gcccgaatcc
     2221 cgctgatagg tttccccgac ggggcctgac ggccccgtaa ccactagaaa ggacgggttc
     2281 acttggcggt cagggtcggc atcaacgggt tcgggcgaat cggccggaac gtgtggcgcg
     2341 cggcgcacgc gcgcggcgcc gacatcgagt gggtcggcgt gaacgacctc accgacgcga
     2401 agaccctggc ccacctcctg aagtacgact cgatcctcgg cccgctcgac gtcgacgtgt
     2461 cggccaccga cagcggcatc gtcatcgacg gcaaggagct ggcggtcttc tccgagcgcg
     2521 acccggccgc gctgccgtgg tccgacgtgg gcgccgacgt ggtgatcgag tccaccggcc
     2581 tgttcaccga ccgcgagaac gcccagaagc acatcgacgc gggcgcgaag aaggtgatca
     2641 tctccgctcc ggccaagggc ccggacgcga cggtggcgct cggcgtgaac ttcgactccg
     2701 cctacgaccg cgacaaccac cacgtgatct cgaacgcctc gtgcaccacg aactgcctgg
     2761 ccccggtagc gaaggtgctg cacgacgcgg tgggcatcga gcacggcgtg atgaccacga
     2821 tccacgccta caccgccgac cagcgcctcc aggacctccc gcacaaggac ctgcgccgcg
     2881 cccgtgcggc cgcgatcaac ctgatcccga cctcgaccgg cgccgcgaag gcgatcggcc
     2941 tcgtggtccc cgagcttgag ggcaagctga acggcatctc cgtccgcgcc ccgctgccca
     3001 ccggctcgat cgtggacctc gtcttcaccg cctcgcgcga gacctccgtc gaggagatca
     3061 acgccgcggt ccgcgagcgg gcggacacgg gcccgctcga gggcatcctc cagtacaccg
     3121 aggacccgat cgtgtcgagc gacatcgtca gctcgcccta ctcgtcgatc ttcgacgcgc
     3181 cgctcacgat ggtgatcgag gggaagctgg tcaaggtctt cgcctggtac gacaacgagt
     3241 ggggctactc gaaccgcgtc gtggagctcg cacagcgggt gctctgaacc ctggtggcgg
     3301 gcttcggcaa gaagaccctg cgcgacatgc agatcggggc gggggaggcg gtcctcgtgc
     3361 gtgtggactt caacgtgccg ctcgcgaacg gcgaggtggc ggacgagacg cgcatccgcg
     3421 cggcgctgcc ggccatcgag cagctgcgcg accgcggcgc ccggctcgtg ctctgctcgc
     3481 acctcggtcg cccgaagctg cgcgagcccg ccacctcgct ggagccggtc tcacgccggc
     3541 tcggtgaatt gctcggcacg cgcgtgcacc aggctcccga ggtggtcgga cccgaggtgc
     3601 gccgcggcgc cgcgcggctc gacccgggcg agatcctcgt gctcgagaac acccgctggg
     3661 aggcgggcga gacgaagaac gaccccgagc tggcccggga gctggccgag ctggccgacc
     3721 actacgtcga cgacgcgttc ggcgccgccc accgcgcgca cgcgagcacc gtcggcgtga
     3781 ccgagcacct gccgtcggct gccggcccgc tgctcgagcg cgaggtcacc acgctcaccg
     3841 agataatcga ggccccgcag cgcccgctcg tggtggtgct tggcggggcc aaggtgagcg
     3901 acaaggccgc cctgatcgac cgcttcctcg acatcgccga cgcgctgctg atcggcggcg
     3961 ctatgtgctt cagcttcttc cgcgcgcagg gcaaggacac cggcgactcg ctggtcgagg
     4021 aggagggcgt cgagcttgcg cgcgaggcgc tcgtgaaggc cgagacctcg gactgccggc
     4081 tgctcttgcc gaccgacctc gtgctgggtg accgcttcga cgcgggcgcc gaggtgcgcg
     4141 agacggcgga ggtctcggtg tcgaagggct ggatgggcct cgacatcggc cgcgagtcgc
     4201 gcgaggtcta cgccgacgag atcgcgaagg ccggcaccgt gttctggaac gggccgatgg
     4261 gcgcgttcga gatggcaacg ttcgcgggcg gcacccgccg cgtggccgag gcggtcgcgc
     4321 aggcccaggg caggacggtc gtgggcggcg gcgactcggc cgccgcactc gtgagcttcg
     4381 ggctcgccga ctccgtgacc cacctctcta cgggtggcgg cgcggcgctc gagctgctcg
     4441 agggcaagcc cctgcccggt gtggaggcac tcgacgatgc ctgatcgcac gccgtacgtc
     4501 gcgggcaact ggaagatgta caagtcggtg gccgagaccg aggagtacat cgggcggctg
     4561 ctcggccgcc tgccggtcgg caacaacgtg accgtgggcc tgtgcgtgcc gttcaccgcg
     4621 ctcgcgaagg ccgtcgagaa ggcggagggc tcgggcgtga aggtgctcgc gcagaccatg
     4681 caccaggctc gcgagggggc attcaccggc gaggtgtcgg cggggatgct cgcggagctc
     4741 ggcgtcgacg gcgtggtgct cggccactcc gagcgccggc gcgactgcgg cgagaccgac
     4801 cgcgcgctcc aggagaaggt gccggccgcg ctcgaggccg ggctcgtgcc gatcctctgc
     4861 gtgggcgagt ccgaggacga gttcgaggcg ggggagaccc agcgcaagct ccgccaccag
     4921 gtccaggagg ggctcgagaa ggtccccgag gacaggctcg ccgacgtcgt gatcgcgtac
     4981 gagccgatct gggccatcgg caccggacgc aacgccacgc ccgagcaggc cgaggaggcg
     5041 atcgggttca tccgcgcgct cgtcggcgac cgctcgaagg aggcggcgca ggccacgcgc
     5101 atcctctacg gcggcagcgt caagtcctcg aacgccgcgg agatcatggc ccaggccgac
     5161 gtcgacggcg cgctcgtggg cggcgcgagc ctcgaccctg ccgagttcgc gaagatcgtg
     5221 gaggcggcgg cgccctgacc gttccctcgg tctgcctcgt catcctcgac gggtgggggc
     5281 tggccccgcc cggccccggc aacgcggtcg agctggccga cacgcctgtg ttcgacgagc
     5341 tctggcgcag ccacccgcgt acccagctca ccgccggcgg ccgcgccgtc gggctgcccg
     5401 agggccagat gggcaattcc gaggtgggcc acctgaacct cggcgcgggg tccgtggtgc
     5461 gccaggacct cacacgcatc gacgatgcgg tcgccgacgg gtcgttcgcg cagaacgagg
     5521 tgctgctcga cgcgatgcgc accgagcggc tgcacctgct cggcctcgtc tccgacggcg
     5581 gcgtgcacgc gagcatggac cacctgcgcg cgctgatcga gctggccgcg gcgcagggcg
     5641 tggcggaggt gctcgtgcac gcgttcaccg acgggcgcga cacgctgccg gactcggggg
     5701 cgggctacgt cgagcaggtc gaggagtggc tcgccgcggc gggcgggcgc atcgcgaccg
     5761 tcagcggccg ctactacgcg atggaccgcg accaccgctg ggagcgcacc gacctggccc
     5821 tcgacgcgat cgtgcacgcg cgctcggagc agccgccggc cgagagcggg gtggccgccg
     5881 tgcgcgccgc ctacgagcgt ggcgagaccg acgagttcgt gaagcccacg gtggtcggcg
     5941 cgggcgggcg ggtccgcgac ggcgactcgc tcgtgttctt caacttccgc ccggaccgcg
     6001 cgcgccagct cagcacgagg ctggccgacc gccgcctcac caccctcacc gagtaccagg
     6061 aggactacac gttcccggtc gcgttccccc ccgagcgccc ggccaccacg ctggcggcga
     6121 ccctcgccga gcgcggcatc ggccagctcc acgtggcgga gaccgagaag tacgcgcacg
     6181 tgacgtactt cttcaacggc ggcgaggagg acgagtacaa gggggaggtg cgctgcctcg
     6241 tcgactcccc gcgcgacgtg ccgacctacg accacaagcc ggagatgagc gcgcgcgccg
     6301 ccgcggacgc attcaccggc cgctggcgcc agggcggctt cggcttcggg atcatcaact
     6361 tcgcaaaccc cgacatggtc ggccacacgg gctcgatccc ggccgccgtg gaggcgatcg
     6421 agaccgtgga cgcgtgcctg gccgacgtgg tcgccgcggt gcacgagtcc ggcggcgcct
     6481 gcatcgtgac cgccgaccac ggcaacgccg acaacatgct cgagcccgac gggagcccga
     6541 acacggcgca ctcgctcaac ccggtgccgc tggtggtcac gaccgccgtg cccgggctgc
     6601 gcgacggcgg cgtgctcgcc gacgtggcgc ccacggtgct cgacctgctg ggccaggagc
     6661 agccggacgc aatgaccggc cgcacgctca tcctgcctaa ctaggacgca tcaaagttga
     6721 caacctggga ctcaggtttc tacactgtgc gccgataagg ggccagaacc ccgtaggacc
     6781 gtaacgagga ggttgttcga tgacccttct gatgaagccc gagcctttct cgagcgactt
     6841 caaccgtctc ttcagcacgc tcttcgacga gaatcgcgtg cagcgctggg tcccgccgat
     6901 ggacctcgtc gaggccgacg accacttcct gctgaaggcc gatctccccg gcctgggcga
     6961 ggaggacgtg gccatcgaga tccgcgacaa caccctcacg atctccggcg agcgcaagtc
     7021 cgagtacgag aagcgcgagc gcggctggta ccgcgtcgag cgcgccaccg gctccttcag
     7081 ccgcgcgctg tcgcttcccg acggcatcga cccggacgcc gtgaaggcgt cgtttgacaa
     7141 gggcgtgctc gaggtgtgga tcccgaagcc cgaggctcgc aagccgcggc ggatcgctat
     7201 caacgcgcac gcgaacggca acggcaacgg cgcgcagccg gtgatcgagg gctgagcgcc
     7261 gcggcagcgc gccctacgct ggcgcggtgc ccgatcttcc gctcgagata ggcgcccgct
     7321 cgggctcggc ccgggcgggc gtcctgcgtt gtgcccacgg cgaggtgcgc acgccggcgt
     7381 tcgtgccgct ggcctcgacg gcgaccgtgc gctcgctcca cgcctccgag gtggccgagc
     7441 tcggctacga catggtgctc ggcaacacgt tccacctgtt cctcgagccc ggccacgagc
     7501 tgatcgagcg gatgggcggg ctgcatgagt tcatggcctg gcgcgggccg atcgtgaccg
     7561 actccggcgg cttccaggtg ttctcgatgg gccacgggtc ggtggccgag gagatcaagc
     7621 gccgccgcgg cgaccgccag agccggatcc tctcgatcga ggaggagggc gtgcgcttcc
     7681 gctcgtacct cgacgggagc gagcgcttca tgggcccgga gacctcgatg gagatccagg
     7741 ccgcgctcgg ctcggacgtc gtgctcgcct tcgacgagtg caccccgttc cacgtcgacc
     7801 gcgactacac ggcgcgcgcg gccgagcgca cccaccgctg gctcgaccgc tgcatctcgt
     7861 ggcggcgcga gcacgcgccc gagggcgggc tcttcatggg gatcgtgcag ggcggcgtcc
     7921 accaggacct gcgggcgcag tcggccgagc gcgtggcgtc gagcgaggtg gacggggtgg
     7981 cggtcggggg gtcgctgggg caggagaagg cgcagatgca cgaggtcgtg gagtggtcgc
     8041 tggcgccggt gcccgacgcg aagccgcgcc acctgctcgg catcggcgac gtggacgaca
     8101 tcctcgccgc cgtcggagcg gggatcgaca cgttcgactg cgcgacgccc acgcgcctgg
     8161 cgcggcacgg gacggccctg gtgagcgacc ccgaggcgcg ctggcggctc gacctgacga
     8221 aggcgcgctg gcgtgagagc cgcgagccga tcgcgccgga ctgcccgtgc ccggcgtgcc
     8281 gcgagcacac gcgcggctac ctgcaccacc tgtcgcgcac cagcgagctg accggcgcgc
     8341 gcctgatcac gctccacaac ctgacgttca tgaaggcgct gatggacggc atccgcacgg
     8401 cgatcgagtc cggcacctac gccgagcacg ccgccgcggt gctgggcggc gcctcgccct
     8461 acggcgccgt tccgggccgg accgcgccct acggctgacg cgggacgacg atcgtcccgg
     8521 cggcgaggtc gccgaggcgc tgcttgcgct tggtcgcgac gatcgtgatc aggccgacga
     8581 ggtagaagaa gaacccgtcg acgatgcgca gcaggttgcg gccgacgatc tggccggtgc
     8641 tcggcttgct gccgtccgcg ctgcgcacga cgaggccgag cgcgcgcttg ccgagcgtcg
     8701 cgttccacac cgcctcggtg acgatgtagt agccgagcag gagcgcgaag tagacgagcg
     8761 cggagccgcc ctcgagcgtg accgacgcgt tgccgtcgcc ggactcgccc tcaccgagtg
     8821 cgatcccgac gatgaagaac agcacgacca tgaggaagaa gtcgatgatc cccgcgccga
     8881 tgcgcttgcc gacgacctcg gtggggtcct gaccgggctg cccgggcggg cctgcggcct
     8941 gccagccctg cggcggcccg ccctgcgggc cccagccctg aggcggcgcg ccctgcgggt
     9001 tccagccctg aggcggcgcg ccct
//
LOCUS       DASVPL010000110         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_92400, whole genome shotgun sequence.
ACCESSION   DASVPL010000110 DASVPL010000000
VERSION     DASVPL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92400"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VF517_05585"
     CDS             <1..748
                     /locus_tag="VF517_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052444.1"
                     /translation="LVGTALLVVAIGAALIAAMRALRLRTGPARAAAAGGVGVFVYWF
                     VHGSVDWFWEMPALGMAAFALLGLAAGLAPRRPPLRPAARRRPLAASYGGVLAAVAAV
                     VVAVSLVGPWLSARYVDDAADVWTTDAGAAFDKLDRAAALNPLSPTPDVIAGHIALRL
                     DRLDEAEARFRAAIEREPVDTRPHLQLGALLVNEGDREVGLRHLRIARRLDPRDEIIR
                     GALARARRGGEIDIEAMNVAIATRYRKLAN"
     gene            748..903
                     /locus_tag="VF517_05590"
     CDS             748..903
                     /locus_tag="VF517_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052445.1"
                     /translation="MRPRWQRILLSVLYWVAVLVVSIAILVALVLLIESRDQSSVENE
                     GAAGLVL"
     gene            complement(920..1573)
                     /locus_tag="VF517_05595"
     CDS             complement(920..1573)
                     /locus_tag="VF517_05595"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX8052446.1"
                     /translation="MLRFVASVLIVSGVLMLADAGVTLGWQEPVSALMAARDQGDLED
                     ELAAQTSSALRDKELTASIRDVKRRIKRLAELDKKRTKEGDALGRIEFPTLDRHYVFV
                     QGTGHDPLRKGPGHYPATSFPGQGGTVAIAGHRTTYGAPFRTIDKLENGQHIEIAMPY
                     GTLEYKVEKKLIVNPDATWITHRVKGAERLVLSACHPLYSASKRIVIFARLVNARVG"
BASE COUNT          206 a          602 c          624 g          255 t
ORIGIN      
        1 cctggtcggc accgctctgc tggtagtggc gatcggtgcg gcgctgatcg cggccatgcg
       61 ggcgctgcgg ctgcgcacgg ggcccgcgcg ggcggcggcg gcgggcggcg tgggcgtatt
      121 cgtctactgg ttcgtgcacg ggtcagtgga ctggttctgg gagatgccgg cgctcgggat
      181 ggcggcgttc gcgctgctcg ggctcgcggc cggcctggcg ccgcgccgcc ccccgctgcg
      241 tcccgcggcg cggcggcggc cgctggcggc gagctacggg ggggtgctgg cggccgtggc
      301 ggcggtggtc gtggcggtga gcctcgtcgg tccctggctg agcgcgcgct acgtcgacga
      361 cgccgccgac gtctggacca ccgacgcagg cgccgccttc gacaagctcg accgggcggc
      421 tgcgctcaac ccgctcagcc cgacgcccga cgtgatcgcc ggccacatcg cgctgcgcct
      481 cgaccgcctc gacgaggccg aggcgcgctt ccgcgccgcg atcgagcgcg agccggtcga
      541 cacgcgcccg cacctccagc tcggcgccct cctcgtcaac gagggcgacc gcgaggtcgg
      601 cctgcgtcac ctgcgcatcg cgcgccggct cgacccccgc gacgagatca tccgcggggc
      661 cctggcacgc gcccggcgcg gcggcgagat agacatcgag gccatgaacg tggcgatcgc
      721 gacccgctac cggaagcttg ccaactgatg cgcccgcgct ggcagcggat cctcctcagc
      781 gtcctctact gggtcgcggt gctcgtggtg tcgatcgcga tcctcgtcgc gctggtgctg
      841 ctgatcgagt cgcgcgacca gtcgtcggtc gagaacgagg gcgcggccgg actggtgctc
      901 tagcccacgc gggcgtgctc tagcccacgc gggcgttcac gaggcgcgcg aagatcacga
      961 tgcgcttcga ggcgctgtag agcgggtggc acgcgctcag cacgagccgc tcggcgccct
     1021 tcacgcggtg ggtgatccag gtcgcgtccg gattcacgat cagcttcttc tcgaccttgt
     1081 actcgagggt gccgtacggc atcgcgatct cgatgtgctg cccgttctcc agcttgtcga
     1141 tcgtccggaa cggcgccccg taggtggtcc ggtggccggc gatcgcgacc gtgccgccct
     1201 ggcccgggaa cgaggtggcg gggtagtggc ccgggccctt gcgcagcggg tcgtgcccgg
     1261 tgccctggac gaacacgtag tggcggtcga gggtcgggaa ctcgatccgg ccgagcgcgt
     1321 cgccctcctt ggtccgtttc ttgtccagct cggcgagccg cttgatgcgc cgcttcacgt
     1381 cgcggatcga ggcggtcagc tccttgtcgc gcagcgcgga gctcgtctgg gcggccagct
     1441 cgtcctccag gtcgccctga tcgcgcgcgg ccatgagggc cgagacgggc tcctgccagc
     1501 ccagcgtgac gcccgcgtcg gcgagcatga ggacgccgct cacgatcagc acggaagcca
     1561 cgaatcgcag caccgctcgg atccatccgc cgccgggacg cacagcggag ccagtgtagg
     1621 cggacccccg gtaccctaag ccgcctcagt cagcgaactc gaggggtgag cccgaccaaa
     1681 tggcgaa
//
LOCUS       DASVPL010000111        12081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_92516, whole genome shotgun sequence.
ACCESSION   DASVPL010000111 DASVPL010000000
VERSION     DASVPL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12081
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92516"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VF517_05600"
     CDS             complement(<1..431)
                     /locus_tag="VF517_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8052447.1"
                     /translation="MNEPENVIIIGSGPAGYTAALYTSRADLNPLLIEGFLWGGLLQT
                     TTDVENYPGFPEGIMGPEMMQKFRDQAERFGTRFISEDATRIELSTDGGLHSVWVGDQ
                     EYKARAVILSMGAQHRKLGVPGEEELASRGVSYCATCDAAF"
     gene            complement(428..1639)
                     /locus_tag="VF517_05605"
     CDS             complement(428..1639)
                     /locus_tag="VF517_05605"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX8052448.1"
                     /translation="MARPRRSRPLGQDWDDSVDEAEELLASGELENDDIEQRSAAMLN
                     PRRLISLVLAIGLMIVAVYVVFPKVVGVSTSLDKLGDATWYWIVSAVGFLGLAFLSYT
                     SLFRGVLGGQDPTDRARQRLDIGASWQITMSGFAATVLFSAAGAGGVALTYWALRQAG
                     IPRRRAACRMVAFLVLLYSVYLMSLIVFGVLLRANVIHGDNPVGGTIIPAAVAGATLL
                     LIGFVALIPQDFERRFAALRRRGGRAARVAARLATAPATLSTGIRTAGDYLRHPRRSA
                     SAVFGALGWWTGQIGILWSCFHAFGVQVPLGVVVQAYFVGMVANLAPSPAAGVGTVDA
                     GLIGAFVLFGIPAETVFPAVLSSRLIGIWLPLPLGILGYVRLRRTIARWREEDRSAAT
                     IQNEVKAETTG"
     gene            complement(1639..2550)
                     /locus_tag="VF517_05610"
     CDS             complement(1639..2550)
                     /locus_tag="VF517_05610"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8052449.1"
                     /translation="MRKSRSLRRVALGAAVVGFVTPVLRRRLALPPAVTSAVAWQTPV
                     AVALSFPRTRVRDAAVYASQMWAYLLHYEMPNDDPAALRERARVDYPVRVDRMIGLGE
                     VPTVRLQRALGREGEVRPHDTALAAIHWAWFFWPHVVVAYVLVRHPERYGRSATLMAA
                     TFDSGAAFYHLIPTAPPWWAGNTGSLPHVRRIMTEAGERVWGRYWSPLYDSLSGNQLA
                     AMPSLHFATSVMAAHVLSDVGRVPGAVGWTYATLLGFALVYLGEHYVVDLAGGAALAE
                     AVRRLEPPARPVARAAAGLVRRLEPAG"
     gene            complement(2582..4336)
                     /locus_tag="VF517_05615"
     CDS             complement(2582..4336)
                     /locus_tag="VF517_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX8052450.1"
                     /translation="MDRTREALKEMAREDPELAARLVVQTLPAAVAKIPPPLAYDLEV
                     EELGTWRVVVEDGGATVSSANGDSANGGSENGDSANGHSPNGDRELDFRLTTDARGLA
                     LMAAGESPARLMLGGKVRIRGKRRRALKLRALAEGDDPSIAEAIRAGAELDVDATFRA
                     LPYLIDPEWTRGHSFVVGYEVEGAGRWLVHVRDGQRLEVTTEGEPGTWLRLSTDTYRG
                     LAAGEITPTQSMREQRTHVEGPMYPATLLGRWIDRAQGRDEAELRREAAQRELHARRA
                     GSWGSAPAARDGDLLDYQQLYALWERQNWRVHELDFSVDKEQWLVSPTEAQENMLWSL
                     GSFYIGEERVAADLAPFVQAAPSGEIEAFLSTQLVDEARHAVFFDRYGAEVLALDAED
                     LRGRMQQIQALMLPAWYEVFDGGLREVAQRIQARPDDFDLFVDGIVVYHLIIEGVLAT
                     TGQRTILDYSNEHGLYPGFCEGFGLVEQDEHRHIAFGVRFLRDAVADDPARGDIVMRR
                     VEELVPKAMHVFCPPYVDSAADFVTYGYHSSQIYGFAYRRLQRRMQIIGLECPPAEDL
                     MPGRVNSLDEARAVGALV"
     gene            complement(4320..4967)
                     /locus_tag="VF517_05620"
     CDS             complement(4320..4967)
                     /locus_tag="VF517_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8052451.1"
                     /translation="MLLDSVEWLGHAGFRVRAGRRLVYIDPYRVEDARPADLILITHG
                     HYDHFSPQDIERLSGEGTLVVAPPAVAERVRGRVVSLAPGEGIDDPLDVRAIAAYNTS
                     KRNPDGKLFHPREAGGVGYVLNVRGERLYHSGDTDVIPEMDEVAGVDVALLAVSGVYV
                     MTAAEAAEAARRIAPRVAVPMHWGEHIGTREDAEAFAHSAPGEVRILDRVHGPDA"
     gene            5002..5799
                     /locus_tag="VF517_05625"
     CDS             5002..5799
                     /locus_tag="VF517_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX8052452.1"
                     /translation="MSDEKTVLWEVTDGIGRVTLNRPDRLNAWIPELGQELLDIFQGP
                     AAADDVRAVLMTGAGRGFSSGADLRAGFDADPDDGGPNVRKELHEVYHPAIVAIRQLP
                     KPVVSAVNGPAVGIGCSLALAGDLILAAESAYFLLAFVNIGLMPDGGSTLFVPAAVGK
                     ARAFQMALLGERVGASQALDWGLVNAVHPDDALLGEAEALAARLAAGPTRSYAASKQA
                     LNRMIYPDMEGQLHLEAELQHKLARTSDFVEGVGAFVEKRQAAFSGS"
     gene            5911..6744
                     /gene="coxB"
                     /locus_tag="VF517_05630"
     CDS             5911..6744
                     /gene="coxB"
                     /locus_tag="VF517_05630"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX8052453.1"
                     /translation="MPRAALIAGGFIVLVGAIFLAQFLPTPEDRSGSPNAEDISTLYS
                     WGIALGTIIFVIVEGVLIYSLVKFRYRRGGPEPAQIRGNTPLEVGWTVGAALFLVVLT
                     TVTFIYLGDIENPQASNPAAQTGSVQYAAIGQEPVPGGKKLQVRVNGQQYLWRYDYNP
                     PGKQQLFSYHDLYVPVGTTVVLKLTSSDVVHSWWIPELGPKTDATPGHTNDTWFRIDQ
                     PGEYDGQCAELCGDNHADMRARVIALPPGEFAAWQERQASDIQRSQSFLSLQRKVRGE
                     N"
     gene            6745..8463
                     /gene="ctaD"
                     /locus_tag="VF517_05635"
     CDS             6745..8463
                     /gene="ctaD"
                     /locus_tag="VF517_05635"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX8052454.1"
                     /translation="MTTAAPPATAAPTSTGNPEVIIRGIKERPTGWLSWLTTTDHKRI
                     GILYIVTTFVFFLLGGVEALLMRLQLAGPDATLLEPRTYNSLLTLHGSTMVFLFIVPV
                     LAGFANYLVPLMIGARDMAFPRLNALSYWLFLFGGIAFYLSIFFTPPSGGWTSYAPLS
                     DDAFLPDGGIDAWIFMIHLTGLSSLVGAINFTATIHNMRARGMSWGRMPLFVWTILIY
                     SYLLILALPVVAGAVTMLLTDRHFGTAFFDNTAGGDPLLWQHLFWFFGHPEVYIMVLP
                     AFGMISEVLPVFARKPIFGYKAIAASTAAIAFLGMLVWAHHMFTTPTATVVLAFFMLS
                     SFAIAVPTGIKIFNWLATLWRGTIMFRTPLYFAAGFLGTFVVGGISGVILAIFPVDWY
                     LHDTYFVVAHMHYVLFGGSVLGIFAGLYYWFPKMSGRMMNERLGKASFWLIFIGFNVT
                     FLVQHSAGLDGMPRRIYWYPESAGWGTYNLISTIGSWILGIGVILTIVNVLFSIKRGQ
                     KAGNDPWKANTLEWFTQSPPPENNFDIVPRVRSVEPMKDIRREVARGTGRSSETVAQP
                     VTTGST"
     gene            8460..9425
                     /locus_tag="VF517_05640"
     CDS             8460..9425
                     /locus_tag="VF517_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX8052455.1"
                     /translation="MSTPTRGFRRLALVTLVVTFLLIVLGGVVRVSDSGLGCGPAKSG
                     FHGWPLCNGDVIPGASIHTAVEYSHRTVASTVGLLALSLLVLARRRYSDHRGIVRAST
                     GAFGLVVAEGLLGAATVEKNLEEELVAAHLGLAMLILALVLYIWRASRPDVIGAPAAD
                     SSVGFRRLAIGVQALILGTIVAGGYMAGTQKYGRADYQLGDGAHHACGRQFPTCNDDF
                     LPFGASRLVDIHLTHRVFMYVATIAIVALVVWALRRRPSGGVVSAARMLGALLLLQLL
                     VGALNVWLEEYEALILVHLTLGALLWATATMLTLQLYPVRALARA"
     gene            9425..10300
                     /locus_tag="VF517_05645"
     CDS             9425..10300
                     /locus_tag="VF517_05645"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX8052456.1"
                     /translation="MEAVRTAPRSVVADYVTLTKPKVQSLLLFTTVTTMYVAGTPSVG
                     TVLLTCLGGALSAGGAGAVNHYLDRDIDATMQRTATRPIPSGRIAPGAALAFGIALGV
                     LSVLLLSLAVNPLAAALSASGYLGYVFVYTIWLKRRTVQNIVIGGAAGAVPPLVAWAA
                     TTGSLSWNAVYLFAIVFYWTPPHFWALSLLMKDEYARASVPMLPVVKGEAETRKQILL
                     YTVLLYAVTQLPFCAGLFDGIYLAASVVLGACFIGGAVLLRRRADRGTALRLYLFSLA
                     YLALLFAAMVADARL"
     gene            10304..11668
                     /locus_tag="VF517_05650"
     CDS             10304..11668
                     /locus_tag="VF517_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8052457.1"
                     /translation="MTRRRALFLAAALLGAVLLAIDARMWDEGGPGIVGFELAWDTRS
                     AERILGEWGPDGRDAARLSLWLDFAFLASYAAFWALASRALRLRVWPLALAAGAFDAL
                     ENVCLLVVLGGSTSGWPLLASSFATLKVLALAVVVGSVAVALVRRAHARRRRAVRAFA
                     AGAALLVVAALAVNTWLVDRATAPAEPGIGQLVEVPAGTIHVSDEGSGPPVVLIHGFG
                     CSLNWWTPVMPLLRKSLRVIRLDLLGHGRSEKPRDGYSMEEQADVVADVMRRLGVHRA
                     AVVGHSMGGMVATALAERHRAMVSRLMLIGTAPDDADEHLGLTARLPFVPVVGHAIDT
                     LVPERLVRLAVEDGFAPGFDPPAEFSRDIFDHTTYRSLTQSARAVSDFWDDGPLHERL
                     RGTHVPLTVLLGEEERHTRRSTGLYNSVPGARTVVMQGLDHTPQVESPARTGPLIAAF
                     AAGR"
     gene            11727..>12081
                     /locus_tag="VF517_05655"
     CDS             11727..>12081
                     /locus_tag="VF517_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052458.1"
                     /translation="MAARRDAARDGLVAAAAQPLACRRRRASGRHHSPAAPERPRRRL
                     AGVRLAGRGGREGRGRARSGGRKRRGFGARLVRRKGRGFGALLDGRKRRGFGARLAVL
                     GGGRCGEWRGARQGWI"
BASE COUNT         1656 a         4363 c         4254 g         1808 t
ORIGIN      
        1 aagaaggccg cgtcgcaggt cgcgcagtag gagaccccgc gcgaggccag ctcctcctca
       61 cccggaacgc ccagcttgcg gtgctgcgcg cccatcgaga ggatcaccgc gcgcgccttg
      121 tactcctggt cgccgaccca cacgctgtgc aggccgccgt cggtcgagag ctcgatccgg
      181 gtggcgtcct cggagatgaa gcgggtgccg aagcgctccg cctggtcgcg gaacttctgc
      241 atcatctccg ggcccatgat cccctcgggg aagcccgggt agttctcgac gtcggtggtc
      301 gtctggagca gcccacccca gaggaatccc tcgatcagaa gggggttgag atcggctcgg
      361 gacgtgtaca gcgcggccgt gtaaccggcg gggccggaac cgatgatgat gacgttctct
      421 ggctcgttca tcccgttgtc tccgccttta cttcattttg tatagtagca gcggagcggt
      481 cctcctcccg ccacctcgcg atggtgcgcc ggagcctcac gtagcccagg atccccagtg
      541 gtagcgggag ccagatgccg atcagcctcg agctgaggac cgccgggaag accgtctcgg
      601 ccggtatgcc gaacaggacg aaggccccga tcaggcccgc gtccacggta ccgacgccgg
      661 cggccggaga cggcgcgagg ttggccacca tgcccacgaa gtaggcctgc acgaccaccc
      721 cgagcggcac ctgcacgccg aacgcgtgga agcacgacca gaggatcccg atctggcccg
      781 tccaccagcc gagcgcgccg aagaccgccg acgcgctgcg ccgcgggtgg cgcaggtagt
      841 cgccggcggt gcggatgccg gtggagagcg tcgcgggtgc cgtggcgagc cgcgcggcca
      901 ctcgcgccgc ccgcccgccc cggcggcgca gtgcggcgaa gcggcgctcg aagtcctgcg
      961 ggatcagcgc gacgaacccg atcagcagga gcgtggcgcc ggccacggcg gccgggatga
     1021 tcgtcccccc caccgggttg tcgccgtgga tcacgttcgc gcgcagcagc acgccgaaca
     1081 cgatcagcga catgaggtag accgagtaga ggagcacgag gaacgcgacc atccggcagg
     1141 cggcccgccg ccgcgggatg cccgcctgcc gcagcgccca gtacgtgagc gccacaccgc
     1201 cggcgccggc cgctgagaac agcaccgtcg cggcgaagcc ggacatcgtg atctgccagg
     1261 acgcgccgat gtcgagccgc tggcgcgccc tgtcggtcgg atcctgcccg ccgagcacgc
     1321 ccctgaacag cgacgtgtac gaaaggaacg cgagcccgag gaacccgacc gccgagacga
     1381 tccagtacca ggtcgcgtcg cccagcttgt cgagcgacgt gctcaccccc accaccttcg
     1441 ggaacacgac gtagaccgcg acgatcatca gcccgatcgc gagcaccagc gatatgagcc
     1501 gccgcgggtt cagcatcgcg gcgctgcgct gctctatgtc gtcgttctcg agctcgcccg
     1561 acgcgagcag ctcctcggcc tcgtcgaccg agtcgtccca gtcctgcccg agcggccggc
     1621 tgcgacgcgg gcgggccatc agccggccgg ctccagccgc ctgacgagcc ccgccgccgc
     1681 gcgggcgacc ggccgcgccg gcggctcgag ccgccgcaca gcctccgcga gcgccgcgcc
     1741 gcccgccagg tcaaccacgt agtgctcgcc caggtacacc agcgcgaacc cgagaagggt
     1801 tgcgtaggtc cagcccacgg cgcccgggac gcgaccgacg tccgacagca cgtgagccgc
     1861 catcaccgat gttgcgaagt ggagcgacgg catcgctgca agctggttgc ccgacaacga
     1921 atcgtaaagg ggtgaccagt agcgccccca gacgcgctcg ccggcctcgg tcatgatccg
     1981 ccggacgtgg ggcagcgagc cggtgttgcc cgcccaccac ggcggcgcgg tggggatgag
     2041 gtggtagaag gccgcgcccg agtcgaacgt ggccgccatc agcgtcgccg agcgcccgta
     2101 gcgctccggg tggcgcacca gcacgtacgc cacgaccacg tgcggccaga agaaccacgc
     2161 ccagtggatc gccgcgagcg ccgtgtcgtg cgggcgcacc tcgccctcgc gcccgagcgc
     2221 gcgctggagg cgcaccgtgg gtacctcccc cagtccgatc atgcggtcca cccgcaccgg
     2281 gtagtcgacc cgcgcgcgct cgcgcaacgc cgcgggatcg tcgttgggca tctcgtagtg
     2341 caggaggtag gcccacatct ggctcgcgta cacggccgcg tcgcgcacgc gcgtgcgcgg
     2401 gaaggagaga gccacggcca ccggcgtctg ccacgccacc gccgatgtga cggccggcgg
     2461 cagtgcgagc cgccggcgga gaaccggggt gacgaagccg accaccgccg caccgagcgc
     2521 tacgcggcgt agggagcgcg acttccgcaa ggcgcggcga gtgtatagac ggggcgtgcg
     2581 gctacacgag cgcgccgacg gcgcgcgcct cgtcgagcga gttgacgcgg ccgggcatca
     2641 ggtcctcggc gggcgggcac tcgagcccga tgatctgcat ccggcgctgg agccggcggt
     2701 aggcgaaccc gtagatctgc gacgagtggt agccgtaggt gacgaagtcc gcggccgagt
     2761 ccacgtaggg cgggcagaag acgtgcatcg ccttcggcac gagctcctcg acccgccgca
     2821 tcacgatgtc gccgcgcgcc gggtcgtccg ccaccgcgtc gcgcaggaag cgcacgccga
     2881 acgcgatgtg gcggtgctcg tcctgctcga ccaggccgaa tccctcgcag aagcccggat
     2941 atagcccatg ctcgttgctg tagtcgagga tcgtgcgctg gccggtggtc gcgagcacgc
     3001 cctcgatgat caggtggtag acgacgatgc cgtcgacgaa caggtcgaag tcgtcgggcc
     3061 gcgcctggat tcgctgcgcc acctcgcgca ggccgccgtc gaacacctcg taccaggcgg
     3121 ggagcatcag ggcctggatc tgctgcatcc gcccgcgcag gtcctccgcg tcgagcgcga
     3181 gcacctccgc gccgtagcgg tcgaagaaca ccgcgtggcg tgcctcgtcc acgagctgcg
     3241 tggacaggaa cgcctcgatc tcgcccgacg gcgccgcctg cacgaacggc gcgaggtcgg
     3301 ccgccacgcg ctcctcgccg atgtagaacg acccgaggct ccagagcatg ttctcctgcg
     3361 cctccgtcgg cgataccagc cactgctcct tgtcgacgga gaagtcgagc tcgtgcacgc
     3421 gccagttctg gcgctcccac agcgcgtaga gctgctggta gtcgagcagg tcgccgtcgc
     3481 gcgcggcggg ggccgagccc cagctccccg cgcggcgggc gtgcagctcg cgctgcgcgg
     3541 cctcgcgccg cagctccgcc tcgtcgcggc cctgcgcgcg gtcgatccag cgcccgagca
     3601 gggtggccgg gtacatcggg ccctcgacgt gggtgcgctg ctcgcgcatc gactgcgtcg
     3661 gcgtgatctc gccggcggcg agcccgcggt aggtgtcggt cgacaggcgc agccacgtgc
     3721 ccggctcgcc ctcggtcgtg acctcgagcc gctgcccgtc gcgcacgtgc acgagccagc
     3781 ggccggcgcc ctcgacctcg tagccgacca cgaagctgtg cccgcgcgtc cactccgggt
     3841 cgatcaggta cggcagcgcc cggaaggtcg cgtcgacgtc gagctccgcc cctgcgcgga
     3901 tggcctcggc gatcgacggg tcgtcgccct cggccagcgc gcgcagcttc agcgcgcggc
     3961 ggcgcttgcc gcggatgcgc accttgccgc cgagcatcag gcgcgccggg ctctcgcccg
     4021 ctgccatcag cgcgagaccg cgcgcgtccg tggtcaggcg gaagtccagc tcgcggtcgc
     4081 cgttgggcga gtggccgttc gccgagtcgc cgttctccga gcctccattc gccgagtcgc
     4141 cgttcgccga cgagaccgtc gcgccgccgt cctccacgac cacccgccag gtgcccagct
     4201 cctcgacctc gaggtcgtac gcgagcggcg gcgggatctt cgcgacggcg gccggaaggg
     4261 tctggacgac cagacgcgcc gcgagctcgg gatcctcccg agccatctcc ttcagcgctt
     4321 cacgcgtccg gtccatgaac cctgtcgagg atcctaacct cgccgggggc ggaatgcgcg
     4381 aacgcctcgg cgtcctcgcg cgtgccgatg tgctcgcccc agtgcatcgg cacggccacc
     4441 cgcggcgcga tccgccgcgc cgcctcggcg gcctccgcgg ccgtcatcac gtacacgccg
     4501 ctcacggcga gcagcgccac gtccacgccg gccacctcgt ccatctcggg gatcacgtcg
     4561 gtgtcgcccg agtggtagag ccgctcgcct cgcacgttca gcacgtagcc gacgccgccc
     4621 gcctcgcgcg ggtggaagag cttgccgtcg gggttgcgct tcgacgtgtt gtaggcggcg
     4681 atcgcgcgca cgtcgagcgg gtcgtcgatc ccctcgccgg gggcgagcga caccacccgg
     4741 ccgcgcaccc gctcggcgac ggccggcggc gcgaccacca gcgtcccctc tccggacagc
     4801 cgctctatgt cctgcggcga gaagtggtcg tagtggccgt gcgtgatgag gatcaggtcc
     4861 gccggccgcg cgtcctcgac ccggtagggg tcgatgtaga cgagccggcg gcccgcgcgg
     4921 acgcggaatc ccgcgtgccc gagccactcc acgctgtcga ggagcatccc cgaaacgcta
     4981 ggcgacctgt acaaagcacg tatgagcgac gagaagacag tcctgtggga ggtcacggac
     5041 ggcatcggcc gcgtgaccct caaccgcccc gaccggctga acgcctggat ccccgagctc
     5101 ggccaggagc tgctcgacat attccagggc ccggccgccg ccgacgatgt gcgcgccgtg
     5161 ctgatgacgg gagccggacg cggcttctcg tcgggcgccg acctgagggc cggcttcgac
     5221 gccgaccccg acgacggcgg cccgaacgtc cgcaaggagc tccacgaggt ctaccacccg
     5281 gcgatcgtcg cgatcaggca gctccccaag ccggtcgtct cggccgtgaa cgggccggcg
     5341 gtggggatcg gctgctcgct ggcgctggcc ggcgacctga tcctggccgc cgaatcggcc
     5401 tacttcctgc tggcgttcgt gaacatcggc ctgatgcccg acggcggctc gaccctgttc
     5461 gtgcccgcgg cggtcggcaa ggcgcgcgcg ttccagatgg cgctgctcgg cgagcgcgtg
     5521 ggcgccagcc aggcgctcga ctggggcctc gtgaacgcgg tgcacccgga cgacgctctg
     5581 ctcggcgagg ccgaggcgct ggcggcgcgg ctcgccgccg ggcccacccg ttcctacgcc
     5641 gcctcgaagc aggcgctgaa ccggatgatc tacccggaca tggagggcca gctccacctc
     5701 gaggccgagc tccagcacaa gctcgcgcgc acgagcgact tcgtcgaggg ggtcggcgcg
     5761 ttcgtggaga agcgccaagc cgccttcagc ggctcctgac gcgcgccccg tccggaatgg
     5821 ccgtcattcc ccaccggact cccggggccc gaccacgtca atagaatgcc gcgccttgcc
     5881 agccgaaggt ccccggagag gtaggcggta gtgccccgcg cagcgctgat cgcgggcggc
     5941 ttcatcgtgc tcgtcggagc gatcttcctc gctcagttcc tgccgacgcc ggaggatcgc
     6001 agcggcagcc cgaacgccga ggacatctcg accctctact cgtggggcat cgccctcggc
     6061 acgatcatct tcgtgatcgt cgagggggtc ctgatctact ccctcgtcaa gttccgctac
     6121 cgccgcggcg ggcccgagcc ggcgcagatc cgcggcaaca ccccgctgga ggtgggctgg
     6181 acggtcgggg ccgcgctgtt cctcgtggtc ctcaccaccg tgaccttcat ctacctcggc
     6241 gacatcgaga acccgcaggc gtcgaacccc gcggcccaga ccggcagcgt gcagtacgcg
     6301 gccatcggcc aggagccggt gcccggtggg aagaagctcc aggtgcgcgt gaacggccag
     6361 cagtatctgt ggcgctacga ctacaacccg cccggcaagc agcagctctt cagctaccac
     6421 gacctctacg tgccggtcgg gacgaccgtc gtcctgaagc tcacctcctc ggacgtcgtg
     6481 cactcgtggt ggatcccgga gctcggtccg aagacggacg ccacgcccgg tcataccaac
     6541 gacacctggt tccggatcga tcagcccggt gagtacgacg gccagtgcgc cgagctgtgc
     6601 ggcgacaacc acgccgacat gcgcgcgcgg gtgatcgcgc tgccgcccgg cgagttcgcg
     6661 gcctggcagg agcgccaggc gtccgacatc cagcgctcgc agtcgttcct gtcgctccag
     6721 cgcaaggtga ggggagagaa ctagatgacg accgctgcgc ctccggccac ggccgccccc
     6781 accagcacgg ggaaccccga ggtgatcatc cgcgggatca aggagcgccc gaccgggtgg
     6841 ctgtcgtggc tgaccacgac cgaccacaag aggatcggga tcctctacat cgtcacgacg
     6901 ttcgtgttct tcctgctcgg tggcgtcgag gcgctgctga tgcgcctcca gctcgccggc
     6961 ccggacgcca cgctgctcga gccgcgcacc tacaactcgc tgctcacgct gcacggctcg
     7021 acgatggtct tcctcttcat cgtgccggtg ctcgcgggct tcgcgaacta cctcgtcccg
     7081 ctgatgatcg gcgcgcgcga catggcgttc ccgcgcctga acgcgctctc ctattggctg
     7141 ttcctgttcg gcgggatcgc gttctacctg agcatcttct tcaccccgcc gagcggcggc
     7201 tggacctcgt acgcgccgct ctcggacgac gccttcctac ccgacggcgg catcgacgcg
     7261 tggatattca tgatccacct caccgggctc tcgtcgctgg tcggcgcgat caacttcacg
     7321 gcgacgatcc acaacatgcg cgcgcgcggc atgagctggg gccgcatgcc gctgttcgtg
     7381 tggacgatcc tgatctactc gtacctgctg atcctggcgc tgccggtggt cgcgggcgcg
     7441 gtcacgatgc tgctcaccga ccgccacttc ggcaccgcgt tcttcgacaa caccgcgggc
     7501 ggcgacccgc tgctgtggca gcacctgttc tggttcttcg gccaccccga ggtctacatc
     7561 atggtgctgc ccgcgttcgg gatgatctcc gaggtcctgc cggtcttcgc gcgcaagccg
     7621 atcttcggat acaaggcgat cgcggcctcg accgcggcga tcgcgttcct cgggatgctc
     7681 gtgtgggcgc accacatgtt caccacgccg acggccaccg tggtgctcgc gttcttcatg
     7741 ctgtcgtcgt tcgcgatcgc ggtgccgacg gggatcaaga tcttcaactg gctggcgacg
     7801 ctgtggcgcg ggacgatcat gttccgcacg ccgctctact tcgcggcggg cttcctcggg
     7861 acgttcgtag tcggcggcat ctcgggcgtg atcctcgcga tcttcccggt cgactggtac
     7921 ctccacgaca cgtacttcgt ggtcgcgcac atgcactacg tgctgttcgg cgggtcggtg
     7981 ctggggatct tcgccgggct ctactactgg ttcccgaaga tgtcgggccg gatgatgaac
     8041 gagcggctcg ggaaggcatc gttctggctg atcttcatcg gcttcaacgt cacgttcctg
     8101 gtccagcact cggcggggct cgacggcatg ccgcggcgga tctactggta cccggaatcc
     8161 gccggctggg ggacctacaa cctgatctcc acgatcggct cgtggatcct cgggatcggc
     8221 gtgatcctca ccatcgtcaa cgtgctcttc agcatcaagc gcgggcagaa ggccggaaac
     8281 gacccgtgga aggccaatac gctcgagtgg ttcacccagt cgccgccgcc ggagaacaac
     8341 ttcgacatcg ttccacgcgt gcgctcggtc gagccgatga aggacatccg gcgtgaggtg
     8401 gcacgcggga cgggcaggtc gtccgagacg gtcgcccagc cggtaacgac cggcagcacg
     8461 tgagcacgcc cacacgcggg ttccgccgcc tggcgctcgt cacgctggtc gtcacgttcc
     8521 tgctgatcgt gctcggcggc gtggtgcgcg tgtcggactc cggcctcggc tgcgggcccg
     8581 cgaagagcgg cttccacggc tggccgctgt gcaacggcga cgtgatcccc ggggcgtcga
     8641 tccacacggc cgtcgagtac tcgcaccgca ccgtggcgtc gacggtcggt ctgctcgcac
     8701 tgagcctgct ggtcctcgcg cggcgccgct acagcgatca ccgcgggatc gtgcgggcct
     8761 ccaccggggc gttcggcctc gtggtcgccg aggggctcct gggggccgcg acggtcgaga
     8821 agaacctcga ggaggagctg gtggccgccc acctcggcct cgcgatgctg atcctcgcgc
     8881 tcgtgctcta catctggcgc gcgtcgcgcc ccgacgtgat cggcgcgccc gcggcggaca
     8941 gctcggtggg cttccggcgg ctggccatcg gggtccaggc gctgatcctc gggacgatcg
     9001 tcgcgggcgg gtacatggcc ggcacgcaga agtacgggcg cgccgactac cagctcggcg
     9061 acggcgccca ccacgcctgc gggcgccagt tccccacctg caacgacgac ttcctgccgt
     9121 tcggcgcctc gcgcctcgtc gacatccacc tgacgcaccg cgtgttcatg tacgtggcga
     9181 cgatcgcgat cgtcgctctc gtggtgtggg cgctgcggcg gcggccgagt ggcggcgtgg
     9241 tgagcgccgc gcggatgctg ggcgccctgc tgctcctgca gctcctcgtc ggggcgctga
     9301 acgtctggct cgaggagtac gaggctctga tcctcgtcca cctcacgctc ggggcgttac
     9361 tgtgggcgac cgccacgatg ctgaccctcc agctctatcc cgtgcgcgcc ctcgccagag
     9421 cctgatggag gcagtccgca cagctccgcg atcggtcgtc gccgactacg tcaccctgac
     9481 gaagccgaag gtgcagtcgc tgctgctctt caccaccgtc acgacgatgt acgtggcggg
     9541 gaccccgtcg gtcggcaccg tgctgctcac ctgcctcggc ggggcgctgt cggccggtgg
     9601 cgcgggggcg gtcaaccatt acctggaccg cgacatcgac gcgacgatgc aacgcaccgc
     9661 cactcggccc atcccgtccg gccggatcgc gcccggggcc gcactcgcct tcggcatcgc
     9721 gctcggagtt ctgtccgtgc tgctgctctc gctcgccgtg aacccgctcg cggcggcgct
     9781 gtcggcatcc ggctacctcg gctacgtgtt cgtgtacacg atctggctga agcgccgcac
     9841 ggtgcagaac atcgtgatcg ggggagcggc gggggccgtg ccgccgctcg tcgcgtgggc
     9901 cgccacgacg ggctcgctgt cgtggaacgc ggtctacctg ttcgcgatcg tcttctactg
     9961 gacgccgccg cacttctggg ctctgtcgct gctgatgaag gacgagtacg cccgcgcgag
    10021 cgtgccgatg ctgcccgtcg tcaagggcga ggccgagacc cgcaagcaga tcctgctgta
    10081 cacggtgctg ctgtacgcgg tcacgcaact cccgttctgc gccggactgt tcgacgggat
    10141 ctacctggcc gcatcggtcg tgctcggcgc gtgcttcatc ggcggggccg tgctcctgcg
    10201 ccggcgcgcc gaccgcggca ccgcgctgcg cctgtacctg ttctccctgg cctacctggc
    10261 gctgctgttc gccgccatgg tggccgacgc gcggttgtag gccgtgacgc gccggcgggc
    10321 gctcttcctc gcagccgccc tgctcggagc agtcctgctg gcgatcgacg cgaggatgtg
    10381 ggacgagggc ggcccgggca tcgtcggctt cgagctcgcg tgggacacgc ggtcggcgga
    10441 gcggatcctg ggcgagtggg ggccggacgg gcgcgacgcg gcgcggctgt cgctgtggct
    10501 cgacttcgcg ttcctcgcgt cgtatgcggc gttctgggca ctcgcgtcgc gtgcgctgcg
    10561 cctgcgggtc tggccgctgg cgctcgccgc cggcgcgttc gacgcgctcg agaacgtgtg
    10621 cctgctcgtg gtgctgggtg ggtccaccag cgggtggccg ctgctcgcgt cgtcgttcgc
    10681 gacgctgaag gtcctggcgc tcgccgtggt ggtcggctcg gtggcggtcg cgctcgttcg
    10741 gcgggcgcac gcgcgccggc ggcgtgcggt gcgggcgttc gcggcgggtg cggcattgct
    10801 ggtggtggcg gcgctcgcgg tcaacacgtg gctcgtggac cgcgccaccg cgccggcgga
    10861 gcccgggatc gggcagctcg tcgaggtgcc ggcggggacc atccatgtga gcgacgaggg
    10921 ctcggggccg ccggtcgtcc tgatccacgg cttcggctgc tcgctgaact ggtggacgcc
    10981 ggtcatgccc ctgctgcgga agagcctgcg cgtgatccgc ctcgacctgc tcggccacgg
    11041 gcgctcggag aagccgcgcg acggctactc gatggaggag caggcggacg tggtggcgga
    11101 cgtgatgcgc cgcctcgggg tgcaccgcgc ggccgtggtc ggccactcga tgggcgggat
    11161 ggtggcgacg gcgctggccg agcgccaccg cgcgatggtg tcgcgcctga tgctgatcgg
    11221 cacggcgccc gacgacgccg acgagcacct ggggttgacc gcacggctgc cgttcgtgcc
    11281 ggtggtgggg cacgcgatcg acacgctcgt ccccgagcgc ctcgtgcggc tggccgtgga
    11341 ggacggcttc gcgcccgggt tcgacccgcc ggccgagttc agccgcgaca tcttcgatca
    11401 cacgacctac aggtcgctga cccagagcgc ccgggcggtc agcgatttct gggacgacgg
    11461 tccgcttcac gagcgcctgc gcggcacgca cgtccccctg accgtcctcc tcggcgagga
    11521 ggagcgccac acgcgccgct cgaccgggct ctacaactcg gtgcccggcg cgcggacggt
    11581 ggtgatgcag ggactggacc acacacccca ggtcgagtcg cccgcccgca ccgggccgct
    11641 gatcgcggcc ttcgcggcgg gccgctgagg ggcaagagtc catttctggg tcctataggg
    11701 gacacaaatg gactcttgcc ctcgccgtgg cggcccggcg ggatgcggcg cgggacggac
    11761 tagtcgcggc cgcggcgcag ccgctcgcct gccgccgacg ccgcgcgagt ggccgccacc
    11821 actcgcctgc ggcgccggaa cgaccccggc gccggctcgc gggcgtccgg ctcgccggac
    11881 gcggggggcg cgaggggcgc ggccgcgcgc ggagcggcgg gcgcaagcgg cgcggcttcg
    11941 gcgcgcggct cgtcaggcgc aaggggcgcg gcttcggcgc gctgctcgac gggcgcaagc
    12001 ggcgcggctt cggagcgcgg ctcgcggtgc tcggcggggg gcggtgcggt gagtggcgcg
    12061 gggcccgcca gggctggatc g
//
LOCUS       DASVPL010000112         5606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_92789, whole genome shotgun sequence.
ACCESSION   DASVPL010000112 DASVPL010000000
VERSION     DASVPL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92789"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VF517_05660"
     CDS             complement(<1..241)
                     /locus_tag="VF517_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052459.1"
                     /translation="MTRRPNSSIIGSPVLIGSIAVLVVVVAVVLAYNANKGLPFVPTF
                     ELNIESSNAARLVVGNDVREGGERIGQVAELSTVRR"
     gene            complement(238..1785)
                     /locus_tag="VF517_05665"
     CDS             complement(238..1785)
                     /locus_tag="VF517_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8052460.1"
                     /translation="MIRRLVIALLAVVAAVAFAAASSDEGVGDGRRYVVELDNAFGMV
                     EEGDVRVAGTNAGKIKGIELDELTKKALVEIELTEDGYGTLRADAECETRPQSPLGEF
                     YMDCRPGHDPRELPAGAHIKRTASTIPPDLITNIMRRPFRERFRLILGELGVGLAGRP
                     EELNDAIRRAVPALRQTSNLLAILADHNRVIKQLVRDADRVVGKLADNKENVGRFVDE
                     AEDTATASAARSSDLAENWRTLPGFLTELTPTMTKLEEVAREERPVLVDLSASAGQLT
                     RFLDISSEFSDASRPALRALADAAPTGREAVRALRPRVRELGAYARPTVNLANDLAIT
                     LEDWDNPARAVERHPQSPGGRGFSGTQALLQYVFIQAIQTNAFDQMGHLSRVSLIQSK
                     CAPLVNAEDYKAREAELKDCRAWLGPNQPGITTPDPTAVPGEGTPPTASGDEQQPETG
                     GTPEPGVPAPSSFRQRGGDARRGGGRQTTVPPEIQQEIERVLDQLEERGQSAPDASQL
                     LDFLLAP"
     gene            complement(1782..3209)
                     /locus_tag="VF517_05670"
     CDS             complement(1782..3209)
                     /locus_tag="VF517_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8052461.1"
                     /translation="MVTGRSVSTRRSAVRVAVLLVLVAAAALVGWLMFYDSGGDYTVR
                     TRFQNAGQVVRGGLVEIGGQKVGTVKKLRLTDDGAAELELSIDDAFAPLPRGTHAQIR
                     QFGLSGPASRYIDLRYPERTGKGDTIEDGGVIEEADTTSNVDLDEVFSIFDKRTRDSL
                     RKVFRGSARQYAGQAENLNQGYLYLDPALVSASRLFRELNRDTGDLERFVTETSGLVG
                     DIADRRDDLASLVSNLADTTGAVARPRSEAGGGALADAIQQLPDFMRQANTTYVNLRA
                     TIDDLDPLVTEFKPVARRLAPYTGEVRRLVQDLDPAVRDLARITTRRGADNDLIDLSL
                     ATLPLRDIAVGPVRRNGAERKGALPATAEALASATPRIAFARPYSNDFTGWLDDFSHT
                     GNIDALGGFSRAASHVSAFSFKNGLVQPLAPIARAAEIENVAQTGQRNKCPGAGERDS
                     FGDGSTPWKPYPEFNCDETQLPLGP"
     gene            3323..3844
                     /locus_tag="VF517_05675"
     CDS             3323..3844
                     /locus_tag="VF517_05675"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8052462.1"
                     /translation="MPVSESLRQSREATVREHMESENRHDFDTTIATFDHPRYELIAT
                     GEVHDGEAEVRDYFADTRTSFPDQSNEPLEFHHSDTAVVVEFILRGTHLGPLRGLPPT
                     GRSFECRMCALFLFDPDGDRIVGERVYFDQATIMRQLGVAHDPASLAGRVSTLVSHPL
                     TIGRALSGRLRRG"
     gene            complement(3910..4524)
                     /locus_tag="VF517_05680"
     CDS             complement(3910..4524)
                     /locus_tag="VF517_05680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8052463.1"
                     /translation="MTRAQAKLYAQKWWWYERNARPVRRLRIHRHMLESGAYIRFPVE
                     GNVLESLDSGRLRLGKGTLLEPGCWITIADEGRVVVGEGCFLNKNTMIAAQNEVTIGD
                     HTMFANGCFISDAAHGYDRDDIPVTWQGFTSKGPTRIGSNCWFGVNCVVNTGVTIGDH
                     SVIGANSVVTKDVPPGVIAAGVPAKVIREIEFRREEAPEAAGPG"
     gene            complement(4521..>5606)
                     /locus_tag="VF517_05685"
     CDS             complement(4521..>5606)
                     /locus_tag="VF517_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052464.1"
                     /translation="SGTRQIPVPRRRRRPAGHIEIEAAAQHNLQKVDARIPLGVFCCV
                     TGVSGSGKSTLVNEVLYKAVANRLHKAKLRPGAHRRVTGLEQVDKIINIDQSPIGRTP
                     RSNPATYTGLFDPIRDLFSRTQEARARGYKPGRFSFNVKGGRCEVCRGDGQIKIEMHF
                     LPDVYVPCEQCGGRRYNRETLEVRFKGKTISDVLQMPVEEALDFFQHIPKIKRRLQAL
                     HDVGLDYIRLGQPATTLSGGEAQRVKLATELSKVGTGRTLYILDEPTTGLHFADVQRL
                     LEMLQRLVEVGNTVVVIEHNLDVIKTADRIIDLGPEGGEEGGTIVAEGTPEQVAATQG
                     THTGRFLSGIVTPKVTKRPRRRLAAAA"
BASE COUNT          734 a         1972 c         2020 g          880 t
ORIGIN      
        1 tgcgccggac cgtcgagagc tcggcgacct ggccgatgcg ctcgccgccc tcgcgcacgt
       61 cgttgcccac gacgaggcgg gcggcgttgg acgactcgat gttcagctcg aacgtcggga
      121 cgaacggcag gcccttgttc gcgttgtacg cgagcacgac cgcgacgacc acgaccagca
      181 ccgcgatcga cccgatcagc acggggcttc cgatgatcga cgagttcggc cggcgggtca
      241 tggcgcgagc aggaagtcga ggagctggct tgcgtcggga gcgctctggc cgcgctcctc
      301 gagctggtcg agcacgcgct cgatctcctg ctggatctcg ggaggcacgg tggtctggcg
      361 cccgccgccg cggcgagcgt cgccgccgcg ctggcggaag gaggacggcg ccggcactcc
      421 gggctcgggc gtcccgcccg tctcgggctg ctgctcgtcg cccgacgccg tcggcggcgt
      481 cccctcaccg ggcacggccg tcgggtccgg ggtggtgatc ccgggctggt tcggcccgag
      541 ccacgcgcgg cagtccttca gctccgcctc ccgggccttg tagtcctcgg cgttgacgag
      601 cggcgcgcac ttgctctgga tcagcgagac gcgcgacagg tggcccatct ggtcgaacgc
      661 gttggtctgg atcgcctgga tgaagacgta ctggagcagc gcctgggtgc cggagaagcc
      721 gcggccgccc gggctctgcg ggtggcgctc gacggctcgc gcggggttgt cccagtcctc
      781 gagcgtgatc gcgagatcgt tcgcgaggtt caccgtcggg cgggcgtagg cacccagttc
      841 gcgtacgcgc gggcgcagcg cgcgcacggc ctcccggccg gtgggggcgg cgtcggccag
      901 ggcgcgcagc gccggccgtg aggcgtcgga gaactcggac gagatgtcga ggaagcgcgt
      961 gagctgaccg gcgctcgcgc tcaggtcgac cagcaccggc cgctcctcgc gcgcgacctc
     1021 ctcgagcttc gtcatcgtcg gcgtgagctc ggtgaggaag cccggcagcg tgcgccagtt
     1081 ctcggcgagg tcgctcgagc gcgcggccga ggcggtggcg gtgtcctccg cctcgtccac
     1141 gaagcggccg acgttctcct tgttgtcggc gagcttgccg accacgcggt cggcgtcgcg
     1201 cacgagctgc ttgatgaccc ggttgtggtc ggcgaggatc gccagcaggt tcgaggtctg
     1261 gcgcagcgcg ggcacggccc ggcggatcgc gtcgttcagc tcctcgggac gcccggccag
     1321 gcccacgccg agctcgccca ggatcagccg gaagcgctcg cggaacgggc ggcgcatgat
     1381 gttcgtgatc aggtcgggcg ggatcgtcga ggcggtgcgc ttgatgtgcg ccccggccgg
     1441 cagctcgcgc gggtcgtggc ccggcctgca gtccatgtag aactcgccga gcggcgactg
     1501 cggtcgggtc tcgcactccg cgtcggcgcg cagggtgccg tagccgtcct cggtcagctc
     1561 gatctcgacc agcgccttct tcgtcagctc gtcgagctcg atccccttga tcttgcccgc
     1621 gttcgtgccg gccacgcgca cgtcgccctc ctccaccatc ccgaacgcgt tgtccagctc
     1681 gaccacgtag cggcggccgt cgccgacgcc ctcgtccgag gacgccgccg cgaacgcgac
     1741 cgccgccacc accgcgagca gcgctatgac gaggcggcgg atcatggccc gagtggcaac
     1801 tgggtctcgt cgcagttgaa ctcggggtac ggcttccacg gcgtcgaccc gtcgccgaag
     1861 ctgtcccgct cgccggcgcc cgggcacttg ttgcgctggc ccgtctgcgc gacgttctcg
     1921 atctcggccg cgcgcgcgat cggcgcgagc ggctgcacca gcccgttctt gaacgagaac
     1981 gccgacacgt ggctggcggc gcggctgaag ccgccgagcg cgtcgatgtt cccggtgtgg
     2041 gagaagtcgt cgagccagcc ggtgaagtcg ttcgagtacg ggcgagcgaa cgcgatccgc
     2101 ggcgtggcgc tggcgagggc ctccgccgtc gccggcagcg cgcccttgcg ctcggcgccg
     2161 ttgcgcctga ccgggccgac cgcgatgtcg cgcagcggga gcgtggccag cgagaggtcg
     2221 atcaggtcgt tgtcggcgcc gcgccgggtg gtgatccgcg cgaggtcccg cacggccggg
     2281 tcgaggtcct gcacgagccg gcgcacctcg cccgtgtagg gcgcgagccg ccgcgcgacc
     2341 ggcttgaact ccgtcacgag cgggtctagg tcgtcgatgg tcgcgcgcag gttcacgtag
     2401 gtcgtgttgg cctggcgcat gaagtcgggg agctgctgga tcgcgtcggc cagggcgccg
     2461 ccgccggcct cgctgcgcgg gcgggcgacc gcgccggtgg tgtcggcgag gttggagacg
     2521 agcgaggcga ggtcgtcacg gcggtcggcg atgtcgccga cgagccccga cgtctcggtc
     2581 acgaaccgtt cgaggtcgcc ggtgtcgcga ttcagctcac ggaacaggcg gctcgcggag
     2641 acgagcgcgg ggtccaggta gagatagccc tggttgaggt tctcggcctg gccggcgtac
     2701 tggcgggcgg agccgcgaaa gaccttgcgc agcgagtcgc gcgtgcgctt gtcgaagatc
     2761 gagaagacct cgtcgaggtc gacgttcgag gtcgtgtcgg cctcctcgat cacgccaccg
     2821 tcctcgatcg tgtccccctt gccggtcctc tccggatagc gcaggtcgat gtagcggctg
     2881 gctggccccg agaggccgaa ctggcggatc tgggcgtggg tgccgcgcgg cagcggcgcg
     2941 aacgcgtcgt cgatcgagag ctcgagctcg gcggcgccgt cgtcggtgag gcgcagcttc
     3001 ttgacggtgc cgaccttctg cccgccgatc tccaccaggc ccccgcgcac cacttggccg
     3061 gcgttctgga agcgcgtcct gacggtgtag tcgccgccgg agtcgtagaa catcagccac
     3121 ccgaccagcg ccgccgccgc gaccagcacg aggagcacgg ccacgcgcac ggcagatcgc
     3181 cgtgtggaca cgctcctccc ggtcaccact agcgcaccac tctccccggc ggcgcaagct
     3241 acacctccgg gcggccgcgc ggcgccagtg gcggtgtgcc acgcgcggga tactcctcgc
     3301 tcccgcccga gaggagatgc aggtgccggt cagcgagtca ctgaggcagt cgcgcgaggc
     3361 gacggtccgc gagcacatgg agtcggagaa caggcacgat ttcgacacca ccatcgcgac
     3421 cttcgaccac ccgcgctacg agctgatcgc caccggcgag gtgcacgacg gcgaggccga
     3481 ggtgcgcgac tacttcgccg acacgcgcac ctcgttcccg gaccagtcga acgagccgct
     3541 cgagttccac cactccgaca cggcggtggt ggtcgagttc atcctgcgcg gcacgcacct
     3601 gggcccgctg cgcgggctcc ccccgaccgg ccgctcgttc gagtgccgca tgtgcgcgct
     3661 gttcctcttc gacccggacg gcgaccggat cgtcggcgag cgcgtctact tcgaccaggc
     3721 gacgatcatg cgccagctcg gggtcgcgca cgacccagcg tcgctggccg gccgcgtctc
     3781 cacgctcgtg tcgcacccgc tcacgatcgg acgcgccctt tcgggccgcc tacggcgcgg
     3841 gtgaagtcaa aagcgtcctc tattgggacg ctatcgactt agggacaatt gtcacttgcg
     3901 ttcgtagcgc tagcccggcc cggcggcctc aggcgcttcc tcgcggcgga actcgatctc
     3961 gcggatcacc tttgcgggca cgcccgccgc gatcacgccc ggcggcacgt ccttggtgac
     4021 cacgctgttc gccccgatca cggagtggtc gccgatcgtc acgccggtgt tcaccacgca
     4081 gttcacgccg aaccagcagt tcgagccgat ccgggtcggg cccttggagg tgaagccctg
     4141 ccaggtcacg gggatgtcgt cgcggtcgta gccgtgggcg gcgtccgaga tgaagcagcc
     4201 gttggcgaac atcgtgtggt cgccgatcgt cacctcgttc tgcgcggcga tcatcgtgtt
     4261 cttgttgagg aagcacccct cgcccacgac cacgcgcccc tcgtcggcga tggtgatcca
     4321 gcagccgggc tcgagcaacg tccccttccc aagccgcagt cgacccgagt cgagggactc
     4381 cagcacgttc ccctccaccg ggaaccggat gtaggccccg gactccagca tgtgccggtg
     4441 gatgcgcagc cgccgcaccg gccgggcgtt gcgctcgtac caccaccact tctgcgcgta
     4501 gagcttcgcc tgggcgcgcg tcacgccgcg gccgccaggc ggcggcgcgg gcgcttcgtg
     4561 accttcggcg tcacgatccc cgagaggaag cggcccgtgt gcgtcccctg cgtggcggcg
     4621 acctgctcgg gagtgccctc ggcgacgatc gtgccgccct cctcgccgcc ctccggaccg
     4681 aggtcgatga tccggtcggc ggtcttgatc acgtcgaggt tgtgctcgat caccacgacc
     4741 gtgttgccca cctccacgag ccgctggagc atctcgagca gccgctggac gtccgcgaag
     4801 tgcagccccg tggtgggctc gtcgaggatg tacagcgtgc ggccggtgcc caccttgctg
     4861 agctccgtgg ccagcttcac ccgctgcgcc tcgccgcccg acagcgtcgt ggccggctgc
     4921 ccgagccgga tgtagtcgag cccgacgtcg tgcagcgcct ggagccggcg cttgatcttc
     4981 gggatgtgct ggaagaagtc gagcgcctcc tcgaccggca tctggagcac gtcggagatc
     5041 gtcttgccct tgaaccggac ctcgagcgtc tcgcggttgt agcggcgccc gccgcactgc
     5101 tcgcacggca cgtagacgtc cggcaggaag tgcatctcga tcttgatctg gccgtcgccg
     5161 cggcagacct cgcagcgccc gcccttcacg ttgaacgaga agcggccggg cttgtagccg
     5221 cgcgcccgcg cctcctgggt gcgcgagaat aggtcgcgga tcgggtcgaa caggccggtg
     5281 taggtggccg ggttcgagcg cggcgtgcgc ccgatcggcg actggtcgat gttgatgatc
     5341 ttgtccacct gctcgaggcc ggtcacgcgc cggtgcgcgc ccggacgcag cttcgccttg
     5401 tgcaggcggt tggcgacggc cttgtagagc acctcgttca cgagcgtcga cttgcccgat
     5461 ccggacacgc ccgtgacgca gcagaagacc ccgagcggga tgcgggcgtc gaccttctgg
     5521 aggttgtgct gggcggcggc ctcgatctcg atgtggccgg ccgggcgccg gcggcggcgc
     5581 ggcacgggga tctggcgcgt gccgga
//
LOCUS       DASVPL010000113         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_288257, whole genome shotgun sequence.
ACCESSION   DASVPL010000113 DASVPL010000000
VERSION     DASVPL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288257"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VF517_05690"
     CDS             complement(<1..970)
                     /locus_tag="VF517_05690"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8052465.1"
                     /translation="MSPSPPTPRGPISEHVLATLARPPHAVEAPPAGGGDPLTDEDLN
                     LALYSLYELHYRGFDGVDETWEWEPSLLAMRAELEARFERGLIEAIGEPSSGAVRPED
                     MDLELRAIADGDDAPSLSIHVERRATADQAREFLIHRSAYQLKEADPHSFALPRLSGG
                     PKAALVEIQADEYGGGRADRVHAQMFADTLAGAGLDNAYGVYLDAIPGFTLATVNLMS
                     LCGLHRRRRGAIIGHLALFEMTSSVPNRRYAAGFRRLGFDDRTCAFFDEHVVADAVHE
                     SVAAVDLAGGLAKQEPALAAQILWGARALVHVEAQWARLILDAWEEG"
     gene            complement(981..2111)
                     /locus_tag="VF517_05695"
     CDS             complement(981..2111)
                     /locus_tag="VF517_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX8052466.1"
                     /translation="MAPAEPDLTADVLRSRFDAAGPLTVGLEEEVLLLDPETHDLTPR
                     AGEVLDRLADPARFKPELPAAQLEIVTAPHERPGGALAELAAGRRNLVEAAAGIARPA
                     ATGLHPFADPEGVLSQSEDYEHTRGEYARIARLQLVSALQVHVAVGGADRSLAVYNAL
                     RSYLPDLAALAANSPYLGGQDTGMATARLKINELLPRQGVPPAIPSWEEFAEALRWGA
                     AAGNVHSPRTWWWELRPHPSHGTLEVRVPDAQTTMEDAAAVVAVVVALVGRLAERHEA
                     GEPLPVAPTWRIEENRWSAARHGSDGTMADLETGERSPTRERLTALLAELGVDEAAAL
                     VERGGASRQRSVAAERGLPGLGGWLADAFLPGPAWAVSPSGT"
     gene            2130..>2355
                     /locus_tag="VF517_05700"
     CDS             2130..>2355
                     /locus_tag="VF517_05700"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX8052467.1"
                     /translation="MRLLRLPGVFKPPSDSWLLARAMRDRGLARDARVLDVFTGSGVL
                     AVAAALAGAREVTAVDVSRRAALCARVNGRL"
BASE COUNT          281 a          875 c          908 g          291 t
ORIGIN      
        1 gcccctcctc ccacgcgtcg aggatgaggc gcgcccactg cgcctcgacg tgcaccagcg
       61 cccgcgcacc ccacaggatc tgcgcggcga gggcgggctc ctgcttggcg agtcccccgg
      121 ctaggtccac ggcggcgacg ctctcgtgca cggcgtcggc caccacgtgc tcgtcgaaga
      181 acgcacacgt gcggtcgtcg aagccgagcc gccggaagcc ggcggcgtag cggcggttgg
      241 gcacgctcga ggtcatctcg aagagggcca ggtggccgat gatcgcgccg cggcggcggc
      301 ggtgcagccc gcagagcgac atcaggttga cggtggcgag ggtgaagccg gggatcgcgt
      361 cgaggtagac gccgtaggcg ttgtcgagcc cggcgcccgc gagggtgtcg gcgaacatct
      421 gcgcgtggac gcggtcggcg cggccacccc cgtactcgtc ggcctggatc tccacgagcg
      481 ccgccttcgg accgccgctg aggcgcggca gcgcgaacga gtgcgggtcg gcctccttga
      541 gctggtaggc ggagcggtgg atcaggaact cgcgcgcctg gtcggcggtg gcgcggcgct
      601 cgacgtggat cgagagcgac ggggcgtcgt cgccgtcggc gatcgcgcgc agctcgaggt
      661 ccatgtcctc gggccgaacg gcgccggagc tcggctcgcc gatcgcctcg atcagcccgc
      721 gctcgaagcg cgcctcgagc tccgcgcgca tggcgagcag cgagggctcc cactcccagg
      781 tctcgtccac gccgtcgaag ccgcggtagt gcagctcgta gagcgagtac agggcgaggt
      841 tgaggtcctc gtcggtcagc gggtcgccac cgccggccgg cggcgcctcg acggcgtgtg
      901 ggggccgcgc gagcgtcgcc aggacgtgct cgctgatcgg gccgcggggg gtgggggggc
      961 tcgggctcac tctcgtcgag ctacgtaccc gatgggctga cggcccacgc aggaccgggg
     1021 aggaaggcgt ccgcgagcca acccccgagc cccggcagcc cgcgctcggc agccaccgac
     1081 cgctgccgac tggcgccccc gcgctccacg agggcggcgg cctcgtccac gccgagctcg
     1141 gccaggagcg ccgtcagccg ctcgcgcgtc gggctgcgct cgccggtctc gaggtcggcc
     1201 atggtcccgt cgctgccgtg gcgggcggct gaccagcggt tctcctcgat ccgccaggtc
     1261 ggcgccaccg gcagcggctc gcccgcctcg tggcgctcgg ccagccgccc gaccagagcg
     1321 acgaccacgg ccacgaccgc cgcggcgtcc tccatcgtcg tctgagcgtc cggcacgcgc
     1381 acctccagcg tgccgtgcga ggggtgcggg cgcagctccc accaccacgt gcgcggggag
     1441 tggacgttcc cggccgcggc gccccagcgc agcgcctccg cgaactcctc ccagctcggg
     1501 atcgccggcg gcacgccctg gcgcggaagc agctcgttga tcttcagccg cgcggtggcc
     1561 ataccggtgt cctgaccgcc caggtacggg gagttcgcgg ccagcgccgc caggtcgggg
     1621 aggtaggagc gcagcgcgtt gtagaccgcg agggagcggt cggcgccgcc caccgcaacg
     1681 tgcacctgga gcgccgatac gagctggagc cgcgcgatcc gcgcgtactc gccccgcgtg
     1741 tgctcgtagt cctcggactg cgacagcacg ccctcgggat cggcaaacgg gtgcaggccc
     1801 gtggcggccg gccgcgcgat gccggcggcg gcctcgacga ggttgcgccg gcccgccgcc
     1861 agctccgcga gcgcgccgcc gggccgctcg tgcggcgccg tcacgatctc gagctgcgcc
     1921 gcgggcagct ccggcttgaa gcgggccggg tcggccagcc ggtcgagcac ctcgcccgcc
     1981 cggggggtca ggtcgtgcgt ctcggggtcg agcagcaaca cctcctcctc gaggcccacg
     2041 gtgagcggcc cggcggcgtc gaagcgcgac cgcaggacgt ccgccgtcag atcgggttcg
     2101 gcgggcgcca cccgggcaac ataggcggcg tgcgactgct gcggctcccc ggcgtgttca
     2161 agcccccgag cgattcctgg ctgctcgccc gcgcgatgcg cgaccgcggc ctggcccgcg
     2221 acgcccgcgt gctggacgtg ttcaccggca gcggggtgct ggcggtggcc gccgcgctcg
     2281 cgggcgcgcg cgaggtgacg gcggtcgacg tctcgcgccg cgcggccctg tgcgcgcgcg
     2341 tgaacgggcg gctga
//
LOCUS       DASVPL010000114        18109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_53537, whole genome shotgun sequence.
ACCESSION   DASVPL010000114 DASVPL010000000
VERSION     DASVPL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18109
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53537"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VF517_05705"
     CDS             complement(<1..407)
                     /locus_tag="VF517_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052468.1"
                     /translation="MPEPRSRARRLVAGGRAAAARVPAVAWLCAATALVVTVAWSLVI
                     PPFHVPDEHAHLAYVQQLAETGDLPRDMPESVLPEETSNALGLTGFFNVIGRTDNRPP
                     ITTLQDRRLQEGIEGAGHTGNGDVDVATPQPPL"
     gene            complement(400..999)
                     /locus_tag="VF517_05710"
     CDS             complement(400..999)
                     /locus_tag="VF517_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052469.1"
                     /translation="MIRLLVVFAAGVAAIASGVVAVMDDRAPRQAGNNGIKPVAFDVV
                     AADRRPACQTGELVPKDAASLEMTIGTFGAPGAPLEATFTKDGRTVTSGRLARGWHEG
                     VVRVPLRPVARTTGGVTVCIRSASRGRIALAGHEGRFRLAYFRAGRESWFDLAGTVTH
                     RFGLGKAAWEGSWTIVLAAFLLLTGLGLALWTMLRQSRA"
     gene            complement(996..2189)
                     /locus_tag="VF517_05715"
     CDS             complement(996..2189)
                     /locus_tag="VF517_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8052470.1"
                     /translation="MASDHMGTTEQTAHARLYDEVPDAPDVSVVIPCLNEAETIEECV
                     SRARAALDGAGLNGEVVVADNNSTDGSGELAEAAGARVVLETRPGYGSAYLAGFGAAR
                     GRYIVMADADLTYDFGEIPHFVEELEQGAELVMGNRMDNIHPGAMPWLHRYIGNPVLT
                     GILNLFFRTGVRDAHCGMRGFRRDVLPRLDLRTTGMEFASEMVIRASKEKLRITEFPI
                     EYHPRGGESKLSSFRDGWRHLRFLLVHSPTYLFVVPGAVMALLGALIGITVLTQIDIF
                     GRQWDIHTMIGAALLMIVGTQVTALGLCAHAYGTYFMGEKDRWFDRMRARYRLEHGLL
                     LGGAIAFGGFVVGAIVVAQWIDRGFGQLSEERLAILGATLIIVGIQIFFSSFLLSILG
                     LRRRP"
     gene            2289..3431
                     /locus_tag="VF517_05720"
     CDS             2289..3431
                     /locus_tag="VF517_05720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8052471.1"
                     /translation="MRVCLVYDCLYPHTIGGAERWYRHLGERLAEDGHDVTYLTLRQW
                     DRGVDPSFGGVRVTTAGPRMELYVEGRRRVLPPLVFGVGVAWHMLRHGRRYDAVHTAS
                     FPYFSLLAIGLAARLHRFRVLVDWHEVWSRAYWEDYLGRAGGRVGWAVQRLCTRVPQH
                     AFCFSRLQERRLREEGFAGAVDVLTGEYAGSLEPSEPRPARPVVVFAGRHIPEKGVTT
                     LVPALAALRERVPGVGLEIYGDGPQREEVLAQIAAAGDDPAVSAPGFVDQPVLEEAMS
                     TALCLVLPSRREGYGLVVVEASARGVPVVVAAAPDNAATELVDDGENGFVAASAEPED
                     LADAIGRVNDAGPELRARTAEWFARNARRLSIESSLATVARRYRSA"
     gene            3433..4572
                     /locus_tag="VF517_05725"
     CDS             3433..4572
                     /locus_tag="VF517_05725"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8052472.1"
                     /translation="MHVGLNLLHLVPAETGGLELYARRLAPALLSAGAGIELTLFVSR
                     EGAPSLAAEPWAQATRVVEVPVNARSRPRRVLAEQTLLPAATRRARVDLLHNMFTTAP
                     ALPGVPQVTTIHDLIYRRVPETHEGILTTGMAVLTRLAALRSQRVLTVSESAAGDIEH
                     HLGIDRDRIDVAYNGPGMDEPAEPLAPYELRRRFELGDARLLLSVSAKRPHKNLERLI
                     EAVAALPAEEDWVLVIPGYPTAYEQRLREHAEARGAGERVRFLGWVDDLALDGLYRAA
                     DCFVFPSLAEGFGLPVLEAMRRGAPVASSDATSLPEVGGDAAVYFDPTDTAAITGAIL
                     RILRDPGLAADLRERGRRQAERFSWEASAAATLRSYERALAMARR"
     gene            complement(4539..5699)
                     /locus_tag="VF517_05730"
     CDS             complement(4539..5699)
                     /locus_tag="VF517_05730"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8052473.1"
                     /translation="MARPLNVGLNLVYLLEQSGGAGTYARELMRGILEVEPDTRLTGF
                     VSAEAPESLLAADWARSVEWVKFPFNVSHGPPGNFALVMGAQWGAVPLIAARRRIDVV
                     HGLANITPLVAPRVATVVTLLDLIWLRFTKTMERRATVGMKLVALPSARRADRVIAIS
                     HSARRDLIERIDLPPERVDVTHLGIRLDETVEPLPEAELRARFELGDRPVVLCVAQKR
                     EHKNLKGLVRAFAALEEPATLVFPGAPTPYEEEMKALAAELGVAERVRFPGWIERDEL
                     EGLYSAAACFVLPSFEEGFGLPILEAMRRGVPVACSNTSSLPEVAGDDALLFDPEKPE
                     EIADAVGRLLRDRDLAARLAERGVERCHRFTWESTARETLAVYRRAIASARS"
     gene            5765..7264
                     /locus_tag="VF517_05735"
     CDS             5765..7264
                     /locus_tag="VF517_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8052474.1"
                     /translation="MTDDPRPGGAPAPADSTDRLSSSSAGPAAIRGGALRVVTYLVGV
                     VLTVGSAAALFRHLGVDDGGRYVVVISLVAIVGGMTDAGLTAIGVRELAVAEPGAQRR
                     LMGQLVGIRVVLTSLAVVGAVAFAAVAGYPSLLVLGTVVAGAGLLVQNVQQTASIPLQ
                     VELRFGWVSVADLLRQAVTAGAILLLVVAGAGLLPFYAVPILAGLAALAVTVALTRGR
                     FRLLPTYDRTAWRSLLRDTLPFALATAIGALYLRVALIVVSLVCTAEQTGYYGVSFRV
                     VDVLVVVPQLVVGAAFPIFARAARDDHARLAYASQRMFEACALLGAGCAVALAGGAGV
                     VIDVVGGSSFGPAADVLRVQGVALALAFVSALTSYLMLSLGMYRQLLLTSLTGFAANV
                     GLVTLLATTHEAIGAAAATVASEAVLVGTGLVLLARTHAGVRPTFGRIPRIVAAAGLA
                     CLALLLPLPGLAVMAVASLAFVVLTLLLRAVPEELLVEVRRRWRPAAAG"
     gene            7275..8234
                     /locus_tag="VF517_05740"
     CDS             7275..8234
                     /locus_tag="VF517_05740"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX8052475.1"
                     /translation="MRVLITGASGFVGRHLADRCRAEGATVVGLSRREPEEGARAALD
                     EHVAADLTDAAATAAAVRAAAPQRVFHLAAAASVTASWSDPRGTLDNNLHGALGLLEA
                     VRAEAPSARVLVASSGEVYGAVDADRLPVTEDHPLRPRNPYAHSKAAVDLLASMHAEV
                     HGLDVVVARAFNHTGPGQSDAYVAGRFARRIAEAEAEGREELQLTTGDLRPRRDFTDV
                     RDVVRAYWLALDRAPAGTYNVCSGAGTQVVDILAALVGQTSLRVEQQTDPALVREHEV
                     MEIRGSNDKLQGATGWRPELALERTLRDTLDWWRERLGAPVPR"
     gene            8231..9217
                     /gene="gmd"
                     /locus_tag="VF517_05745"
     CDS             8231..9217
                     /gene="gmd"
                     /locus_tag="VF517_05745"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX8052476.1"
                     /translation="MSRTALITGITGQDGSYLADLLLEKGYEVHGMVRRASSETFQRL
                     AHIRDSITLHQGDLLDQSSLTDVLRACEPDEIYNLAAMSYVPTSWTQPVLTAEFSGTG
                     VTRILEAMRAAAPDARFYQASSSEMFGKVREVPQTEMTPFYPRSPYGVAKAYGHFITV
                     NYRESYDLHCSSGILFNHESPRRGLEFVTRKVTHGVAAIKLGMADHLALGNLDAERDW
                     GYAKDYVEAMWMMLQQDEPDDYVIATGTTYSVRQLVELSFEHVGLDPDDYVRMDPRFL
                     RPAEVDHLIGDPAKAREKLGWEPRTSFPELVKLMVDADLELLAAGVPQQQAG"
     gene            9222..10235
                     /locus_tag="VF517_05750"
     CDS             9222..10235
                     /locus_tag="VF517_05750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8052477.1"
                     /translation="MRTELLEQLRCPRCQVERSFDVTVAEEDEREIRAGELTCRACNH
                     TATIEDGIVDLLFEPPEFVTREAAGLGRFVDQMVADGWDRERILKLPYEQSGYWFSQA
                     AAMEHLLEINPPRPGATLLDIGSNTCWASNIFAKRGLRVTALDISTPHMQGLKTADWW
                     MEENDIYFERILSVMFDPALATGSYDYVFCCEVLHHNHRENLQRTLRELYRILKPDGK
                     LIVLNEPLKFPTNRKEDHAQEVAEFEGHEHVYYMHEYLLAAYRAGFKPSIVRPNTTPF
                     FRGEEVVLRQQEPMKASARKMARFWIRKLPRARRAYTAYKLAIGPDDLSLGMLCTKRA
                     SGG"
     gene            10232..11218
                     /locus_tag="VF517_05755"
     CDS             10232..11218
                     /locus_tag="VF517_05755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX8052478.1"
                     /translation="MSPAGRTVSVAIPVRNGGRSFVALLDALGAQRIDREVEVVVADN
                     ESTDGTREEAARRGATIVDVPQGEFSHGGTRDLLMERARGEHVAFVTDDVLPVGDSWL
                     AELVAGFDDAERVGLVYGPYVPRPDASHMVSRELTEFFLSLSPDGTPRVDRGLRPEDV
                     RPGPVTFYTDANGCVARAAWERVRFRPVRYAEDQLLALDMLRAGFAKVYRPRAAVIHS
                     HDYPLRERFGRFFDEFRALREVYGHVEEAGWSYTPAVVRAGVRRDRGFLRAQGRSGWE
                     VDRGTLESLVYHGIRATAAILGTRADRLPPRVRRALSRERRDQFEPLERGLV"
     gene            11269..13260
                     /locus_tag="VF517_05760"
     CDS             11269..13260
                     /locus_tag="VF517_05760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8052479.1"
                     /translation="MSPRRRRLEALRLRMVDVFGNLPKRAYLTLRYRGVGSFLFRAIT
                     FPLRLTPAARFLPVGRDRTYAAAARRWYELNGRPVTIAIPHWGPTDQTVETVQALLGT
                     TDPERVRIVVADDASPAPYPEELRRRLAGMAEVILGEENGGFSVNANRAIRAARPGDD
                     VVLLNSDVTALPGWLEALQYAAYGSDDIGLVGPKLLYPDRTIQSAGSHRNQADPIWFD
                     HRYRFKPLDHPPAEFQAPCIAMTGACIYAKRTTIDTIGLLDERYPMAYEDVDWCLRAW
                     QAGLRVIYQPFSAMFHLESKTRGTEQGERELKSQRLFWEEWGDWFDRRQVRTPEGKLR
                     IVYVTEDTGVGGGHRVVFEHLNGLAARGHECELWSLDTAPGWFDLDVPVRTFEDYDAL
                     TEALEPLEAIKVATWWNTAAPVWRASVVYGLPVYFVQDIETSYYPNHPHMHGSVMNSY
                     RQEFRYLTTSDWVAGKLRELHLDPHIVPPGVDLETFHELDVEREDNVVLTLGRSNPLK
                     NLDLTLDAWSSMPNGRPELWMFGIEPELGERPGARYFVRPSDAEVNDLMNRTTALVQT
                     SRHEGFCLPLLEAMAAGAPVVCTDAHGNRDFCRDGDNCLMVHDDPASVRAALERLMRD
                     RQLRERLSAEGRRTAERYAWPGLIDQVERFFEDVAPDPV"
     gene            13257..14570
                     /locus_tag="VF517_05765"
     CDS             13257..14570
                     /locus_tag="VF517_05765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8052480.1"
                     /translation="MRVGVLIHEFGRSGGAQTVLRYARALEDAELLVTAPRARDLPAE
                     VDGVPVRHVSEAGGRRYDCAIATWWTTAESLWEVEADRRLVFLQSIEQRFYEEHHWYE
                     RFAAEEVLALPVGYVVVAPWMRDLLAELRPDAPCVVVRNGIDKEMFRPRGGGGDRADG
                     PLRVLVEGQPTMWFKGVRESLAAVRAMREPVEATLVAGDPDSAGGVDGVRVVGGLDPA
                     GMAEVYAAHDVVLKLSRVESLGLGPIEGFHVGLPAVVTPYTGHEEYLDHDRNGLVVGF
                     DDVPGTARALDSLAADRGRLRRLAEGALATAASWPAADAAASAFEDAIRELCGGPPPD
                     ADGALTQLQRAQRRWLELSREHARQELTVAEGLRGSVRWYEHALAEAAEHHRELDRQR
                     HEVSERASVFEAELARRTEQLEELKATRAYRAAAALRRLKPGRDA"
     gene            14567..15340
                     /locus_tag="VF517_05770"
     CDS             14567..15340
                     /locus_tag="VF517_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052481.1"
                     /translation="MTPLRVLGDDRALALAGPPGAEPAAGPAVHLGEPSTEPAPGDVV
                     WLPGPASEPPCAGVRLVATGGDALWSRAPWPARDELFDLALPTDECALVVGADDARRA
                     VVVEKLEARGRPVLVAPQLSAGDLARVSVVALLGDADAPAPASPWSARAMPAEAPAVL
                     AARRVLVAPRCGLTFGLLPGSDHLAFGAEDDVVSYIDAVLTFPQAFEPLPALGALAAE
                     RHRASRVYARLALELRAAADPAAADDPAAADPAAADPAG"
     gene            complement(15337..16182)
                     /locus_tag="VF517_05775"
     CDS             complement(15337..16182)
                     /locus_tag="VF517_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8052482.1"
                     /translation="MSTAESAFGKRIRGPSALGGDWRRFLHLTWTLAITDFKLTFFGS
                     FLGYLWQLMRPLMLFTVLYFVFTEFVRIGQQAEHFAVVLLMSIVIFTFFTDSTAAAIS
                     SLVDRENLVRKIHFPRMVIPLAVVLTAFLNLTINLAAVGVFALAQGVEPRWSWLEFPV
                     LMLALGTWAVGLSMLLSALYVRARDVQPIWQVISQAMFYAAPVIYPIEAIPKPEWGEL
                     VMLNPVSVFLEQTRHAVIDPSAPTAAAAAGPTGLLIPIAIMVLTTVLGFWLFNREAPH
                     IAEQI"
     gene            complement(16179..17432)
                     /locus_tag="VF517_05780"
     CDS             complement(16179..17432)
                     /locus_tag="VF517_05780"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8052483.1"
                     /translation="MSPPPDSELPAVVVDGVSKTFRKPHEAVHTFKERALHPFRRTGH
                     DQFEALKDVSFAVPKGEFFGIVGRNGSGKSTLLKCMAGIYGVDTGAIYVNGRVSTFIE
                     LGVGFNPDLAARDNVALNAVMLGLSPKDARERFERVIDFAELHEFVDLKLKNYSSGMQ
                     VRLAFSVMIEVDADILLIDEVLAVGDASFQQKCFDVFHRMREEGRTILFVTHDMGAVK
                     RFCDRALLIERGNMRMIGPPDEVASNYLELNFGREGGRDEESGEERYGDQTAQIAEAW
                     FEDDDGERQTALPQGEPCSFRMRVVFQREIVNPALAVLLENEAHLPLFATSTDAADWG
                     TGSYSAGDEVVFSVQFENVFAPGRLYASPWVTTGPPGNHLLDRRPQLATVVSTGAHAT
                     GGLVNFDHDVTIETPSGEKLTARAT"
     gene            complement(17429..>18109)
                     /locus_tag="VF517_05785"
     CDS             complement(17429..>18109)
                     /locus_tag="VF517_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8052484.1"
                     /translation="LDHERDGVPVHTGCAVTGVAAGGRRLVLRHSRGELRARHAIFCA
                     GLWSDRLARTASGPRDPHVVPFRGGYLRLRPERRELVRSLIYPVPDPRLPFLGVHLTR
                     HVDGEVTIGPTALLVGGLRFGGTWRLMGRFWRTGLSELRHAGSRRSLVRAAARFVPEL
                     GPADVLPGPSGVRAQAVGRDGRLLDDFVLSETERALHVRNAPSPAATSAFAIARLVAE
                     RAELHLPR"
BASE COUNT         2449 a         6617 c         6578 g         2465 t
ORIGIN      
        1 tagagcggcg gctgaggggt cgccacgtcc acgtcgccgt tgccggtgtg cccggcgccc
       61 tcgatccctt cctggagcct gcggtcctgg agggtcgtga tcggtggccg gttgtcggtc
      121 cggccgatca cgttgaagaa cccggtgagg ccgagcgcgt tcgacgtctc ctccggcagc
      181 accgactcgg gcatgtcgcg cgggaggtcg ccggtctcgg cgagctgctg cacgtacgcg
      241 aggtgggcgt gctcgtcggg cacgtggaac ggcgggatca cgagcgacca ggcgaccgtc
      301 accacgagag cggtggcggc gcagagccac gcgaccgccg gcacgcgtgc ggcggccgcg
      361 cggccccccg ccacgaggcg ccgggcgcgc gagcgcggct caggcacgcg actggcgcag
      421 catcgtccag agggcgaggc ccagcccggt gaggagcagg aaggcggcga ggacgatcgt
      481 ccacgacccc tcccatgcgg ccttgccgag gccgaaccgg tgcgtgaccg tgcccgccag
      541 gtcgaaccac gactcgcgcc cggcgcggaa gtacgcgagg cggaagcgcc cctcgtggcc
      601 ggccagggcg atgcgcccgc gcgaggcgct gcgaatgcag accgtcacgc cgccggtggt
      661 gcgggcgacg gggcgcagcg gcacccgcac cacgccctcg tgccagccgc gcgcgagccg
      721 gccgctggtc acggtcctgc cgtccttggt gaacgtggcc tcgagcggcg cccccggtgc
      781 cccgaaggtg ccgatcgtca tctcgagcga cgcggcgtcc ttcggcacga gctcgccggt
      841 ctggcacgcc ggacggcggt ccgccgcgac cacgtcgaac gccaccggct tgatcccgtt
      901 gttgccggcc tggcgcggcg cccggtcgtc catgaccgcc acaaccccgc tcgcgatcgc
      961 ggccacgccg gccgcgaaga ccacgagcag tctgatcatg gacggcggcg aagacccagg
     1021 atgctcagga ggaacgacga gaagaagatc tggatgccga cgatgatcag cgtggcgccg
     1081 aggatcgcga gccgctcctc ggagagctgg ccgaagccgc gatcgatcca ctgcgcgacc
     1141 acgatcgccc cgacgacgaa cccgccgaac gcgatggcgc cgcccagcag caggccgtgc
     1201 tcgagccggt agcgcgctcg catccggtcg aaccagcggt ccttctcgcc catgaagtac
     1261 gtgccgtacg cgtgcgcgca caggccgagc gccgtgacct gcgtgccgac gatcatcagc
     1321 agcgccgcgc cgatcatcgt gtggatgtcc cactggcgcc cgaagatgtc gatctgcgtg
     1381 agcaccgtga taccgatgag cgcaccgagc agcgccatca ccgcgccggg caccacgaac
     1441 aggtacgtgg ggctgtgcac gagcaggaag cgcaggtggc gccagccgtc gcggaagctc
     1501 gacagcttcg actcgccgcc gcgcgggtgg tactcgatcg ggaactcggt gatccgcagc
     1561 ttctccttcg aggcccggat gaccatctcg gacgcgaact ccatgccggt ggtgcgcagg
     1621 tcgagccgcg ggagcacgtc gcgccggaag ccgcgcatgc cgcagtgtgc gtcgcgcacg
     1681 ccggtgcgga agaacaggtt gaggatcccg gtcaggacgg ggttgccgat gtagcggtgc
     1741 agccacggca tcgcgcccgg gtggatgttg tccatccggt tgcccatcac gagctccgcg
     1801 ccctgctcta gctcctccac gaagtgcggg atctcgccga agtcgtaggt caggtccgcg
     1861 tccgccatga cgatgtagcg gccgcgggcc gcgccgaacc cggcgaggta ggcgctgccg
     1921 tagccgggcc gcgtttcgag cacgacgcgc gcgccggccg cctccgccag ctcgccgctg
     1981 ccgtcggtcg agttgttgtc cgccaccacg acctcgccgt tgaggccggc gccgtcgagc
     2041 gccgcgcggg cccggctcac gcactcctcg atcgtctccg cctcgttcag gcaggggatg
     2101 acgaccgaga cgtccggcgc gtccgggacc tcgtcataga gtcgtgcgtg agcggtctgc
     2161 tccgtggtgc ccatgtggtc gctcgccatc gacgcaagca gattcgggaa cgcgcgccaa
     2221 aggcgaaagt tgcgcgccgg cgctttttga ggttagcacc ggcctctgtg ccaacctgac
     2281 ccgcttccat gcgcgtctgc ctcgtctacg actgtctcta cccgcacacg atcgggggag
     2341 ccgagcgctg gtatcgccac ctcggcgagc gcctcgccga ggacggtcac gacgtcacgt
     2401 acctcacgct gcggcagtgg gaccgggggg tcgatccgtc gttcggcgga gtgcgggtca
     2461 cgaccgcggg cccgcgcatg gagctctacg tcgagggccg ccgccgcgtg ctgccgccgc
     2521 tcgtgttcgg tgtgggcgtg gcgtggcaca tgctccgcca cgggcgccgc tacgacgcgg
     2581 tccacaccgc ctcgttcccg tacttctcgc tgctcgcgat cggcctggcc gcgcggctgc
     2641 accgcttccg cgtgctggtg gactggcacg aggtctggtc gcgcgcgtac tgggaggact
     2701 acctcgggcg ggcgggcggc cgcgtcggct gggcggtgca gcggctctgc acccgggtgc
     2761 cgcagcacgc gttctgcttc tcgcgcctcc aggagcgcag gctgcgcgag gagggcttcg
     2821 ccggcgcggt ggacgtgctc accggcgagt acgcgggatc gctcgagccg tccgagccgc
     2881 gccccgcgcg cccggtcgtg gtgttcgccg gccggcacat ccccgagaag ggggtcacca
     2941 cgctcgtgcc cgcgctcgcg gcgctgcgcg agcgggtgcc gggcgtgggg ctcgagatct
     3001 acggcgacgg gccgcagcgc gaggaggtgc tcgcccagat cgccgccgcg ggggacgacc
     3061 ccgcggtgag cgcgcccggg ttcgtggatc agccggtgct cgaggaggcg atgagcacgg
     3121 cgctgtgcct ggtgctcccg tcgcgccgcg agggctacgg cctggtggtc gtggaggcct
     3181 ccgcgcgcgg cgtccctgtc gtggtggcgg ccgcgccaga caacgcggcc accgagctcg
     3241 tggacgacgg cgagaacggg ttcgtcgcgg cctcggccga gcccgaggac ctcgcggacg
     3301 cgatcgggcg ggtgaacgac gccgggcccg agctgcgcgc gcgcaccgcc gagtggttcg
     3361 cgcgcaacgc gcggcggctc tcgatcgagt cctccctggc caccgtcgcc cgccgctaca
     3421 ggtccgcctg aggtgcacgt cgggctcaac ctgctccacc tcgtccccgc cgagaccggc
     3481 ggtctcgagc tgtacgcccg ccgcctcgcg cccgccctgc tgagcgcggg ggccgggatc
     3541 gagctgaccc tgttcgtctc gcgcgagggc gctccgtcgc tggccgccga gccgtgggcg
     3601 caggcgacgc gggtggtcga ggtgccggtg aacgcccgca gccggccgcg ccgcgtgctc
     3661 gccgagcaga cgctcctgcc ggccgcgacg cgccgggcgc gcgtggacct gctccacaac
     3721 atgttcacca cggcgcccgc gctgcccggc gtgccccagg tcacgacgat ccacgacctg
     3781 atctacaggc gcgtgccgga gacccacgag ggaatcctca caaccggcat ggcggtgctc
     3841 accaggctcg ccgccctccg ctcgcagcgc gtgctgacgg tgtcggagtc cgccgccggc
     3901 gacatcgagc accacctcgg gatcgaccgc gaccggatcg acgtggccta caacgggccg
     3961 gggatggacg agccggccga gccgctggcg ccgtacgagc tgcgccgccg cttcgagctg
     4021 ggcgacgcgc ggctgctgct cagcgtgtcc gccaagcgcc cgcacaagaa cctcgagcgg
     4081 ctgatcgagg cggtcgccgc gctaccggcc gaggaggact gggtgctcgt gatcccgggc
     4141 tacccgaccg cctacgagca gcgcctgcgc gagcacgccg aggcgcgtgg ggcgggtgag
     4201 cgcgtgcgct tcctcggctg ggtcgacgac ctcgccctcg acgggctcta ccgggcggcg
     4261 gactgcttcg tgttcccgtc gctggccgag ggcttcgggc tgccggtgct cgaggcgatg
     4321 cgccgcggcg cgcccgtcgc gtcctcggat gccacctcgc tgcccgaggt cggcggggac
     4381 gccgccgtgt acttcgaccc caccgacacc gccgcgatca ccggcgcgat cctgcgcatc
     4441 ctgcgcgacc cggggctggc cgcagacctc cgcgagcgcg gccgccggca ggccgagcgc
     4501 ttcagctggg aggcgtccgc cgccgcgacg ctgcgcagct acgagcgcgc gctggcgatg
     4561 gcccggcggt agaccgcgag cgtctcccgc gccgtcgact cccaggtgaa ccggtggcag
     4621 cgctcgaccc cccgctcggc cagccgcgcg gcgaggtcgc gatcgcgcag caggcggccc
     4681 accgcgtcgg cgatctcctc cggcttctcg gggtcgaaca ggagcgcgtc gtcgcccgcg
     4741 acctcgggca gcgacgacgt gttcgagcac gccaccggga ccccgcgccg catggcctcg
     4801 aggatcggaa gcccgaagcc ctcctcgaac gacggcagca cgaagcaggc cgcggcgctg
     4861 tagagcccct cgagctcgtc gcgctcgatc cagccgggaa agcgcacgcg ctccgcgacg
     4921 cccagctcgg cggccagggc cttcatctcc tcctcgtagg gagtgggggc gcccgggaac
     4981 accagcgtcg ccggctcctc gagagcggcg aacgcgcgca cgagcccctt gaggttcttg
     5041 tgctcgcgct tctgggcgac gcacagaacg accggccggt cgcccagctc gaagcgcgcg
     5101 cgcagctcgg cctccggcag cggctcgacg gtctcgtcga ggcggatccc gaggtgcgtc
     5161 acgtcgaccc gctcgggcgg caggtcgatc cgctcgatca ggtcgcggcg agcggagtgc
     5221 gagatcgcga tcacgcggtc ggcgcgccgc gccgagggca gcgcgacgag cttcatgccc
     5281 accgtcgcgc ggcgctccat cgtcttcgtg aagcgcagcc agatcaggtc gagcagcgtc
     5341 acgaccgttg ccacgcgcgg cgcgacgagc ggcgtgatgt tcgccaaccc gtgcacgacg
     5401 tcgatgcgac gccgcgccgc gatcagaggg actgcgcccc actgcgcgcc catgaccagc
     5461 gcgaagttcc ccggcggccc gtgggagacg ttgaacggga acttgaccca ctccaccgag
     5521 cgcgcccagt ccgcggcgag caggctctcg ggcgcctcgg cgctgacgaa gcccgtgagg
     5581 cgggtgtccg gctcgacctc gaggatcccg cgcatcagct cgcgtgcgta ggtgccggcg
     5641 ccgccggact gctcgaggag gtagacgagg ttgagcccga cgttcagcgg acgtgccatg
     5701 gcgcggcgaa gctagcaagc cgttccggcc cggagggggc cggacgcaga cctaacatgg
     5761 cgtcgtgacc gacgaccccc gccccggcgg cgctcccgcc ccggcggact cgaccgaccg
     5821 tctctccagc tcgagcgccg gtcccgccgc gatacggggc ggcgcgctgc gggtggtcac
     5881 gtacctcgtc ggcgtggtgc tcacggtcgg ctcggcggcg gcgctgttcc gccacctcgg
     5941 cgtcgacgac ggcggccgct acgtggtggt gatctcgctc gtggccatcg tgggcgggat
     6001 gaccgacgcg ggcctcaccg cgatcggcgt gcgcgagctg gcggtggccg agccgggcgc
     6061 gcagaggagg ctgatggggc agctcgtcgg catccgcgtg gtgctcacgt cgctggcggt
     6121 cgtcggggcg gtggcgttcg ccgccgtcgc cggctacccg tcgctgctcg tgctcggcac
     6181 ggtcgtggcc ggcgccggac tgctcgtgca gaacgtgcag cagaccgcga gcatcccgct
     6241 tcaggtcgag ctgcgcttcg gctgggtgtc ggtcgcggac ctgctgaggc aggcggtgac
     6301 cgcgggcgcg atcctgctgc tcgtagtcgc gggcgccggc ctgctgccgt tctacgcggt
     6361 cccgatcctg gcggggctcg ccgcgctcgc cgtcaccgtc gcgctcacgc gcgggcgctt
     6421 ccggctgctg cccacctacg accgcacggc gtggcggagc ctcctgcgcg acacccttcc
     6481 gttcgccctc gccacggcga tcggcgcgct ctacctccgg gtcgcgctga tcgtggtgtc
     6541 gctcgtctgc accgcggagc agaccggtta ctacggggtc tcgttccgcg tggtcgacgt
     6601 gctcgtcgtg gtcccccagc tcgtggtggg ggcggcgttc ccgatcttcg cgcgcgccgc
     6661 ccgcgacgac cacgcccggc tggcgtacgc gtcgcagcgg atgttcgagg cctgcgcgct
     6721 gctgggggcg gggtgcgcgg tcgccctcgc gggcggcgcc ggcgtggtga tcgacgtggt
     6781 ggggggatcg tcgttcgggc ccgccgcgga cgtgctccgg gtccagggcg tcgcgctggc
     6841 cctcgccttc gtcagcgcgc tcaccagcta cctcatgctg agcctcggca tgtacaggca
     6901 gctcctgctc acctcgctga cgggcttcgc ggcgaacgtc gggctcgtga ccctgctcgc
     6961 caccacgcac gaggcgatcg gagccgccgc ggccacggtt gcgagcgagg ccgtgctggt
     7021 gggcaccggg ctcgtcctgc tcgcccgcac ccacgcgggc gtgcggccca ccttcggccg
     7081 catcccgcgg atcgtggccg ccgcgggcct ggcctgcctc gccctgctcc tgccgttgcc
     7141 cgggctggcc gtcatggccg tggcgtcgct cgccttcgtg gtgctcacgc tgctgctccg
     7201 cgccgtaccc gaggagctgc tcgtggaggt ccggcggcgc tggcgccccg ccgccgccgg
     7261 gtagcgtggc ttcgatgcgc gtgctcatca cgggggcgtc cggcttcgtc ggccggcatc
     7321 tggcggaccg ttgccgcgcg gagggagcga ccgtcgtcgg cctctcgcgc cgggagcccg
     7381 aggagggcgc gcgcgcggcg ctcgacgagc acgtggcggc cgacctgacg gacgccgccg
     7441 caaccgccgc ggcggtgcgc gccgcggcgc cgcagcgcgt gttccacctc gccgccgccg
     7501 cgtcggtgac cgcgtcctgg agcgatccgc gcgggacgct cgacaacaac ctgcacggcg
     7561 cgctcgggct gctcgaggcc gtgcgcgccg aggccccgtc cgcgcgggtc ctggtggcga
     7621 gctccggcga ggtgtacggg gcggtcgatg ccgaccggct cccggtgacg gaggaccacc
     7681 cgctgcgccc gcgcaacccg tacgcgcaca gcaaggccgc cgtggacctg ctcgcctcga
     7741 tgcacgcgga ggtgcacggc ctcgacgtgg tggtggcccg ggccttcaac cacacggggc
     7801 cgggccagag cgacgcctac gtggccggcc gcttcgcccg ccggatcgcc gaggccgagg
     7861 ccgagggccg cgaggagctc cagctcacga ccggcgatct gcgcccccga cgcgacttca
     7921 cggacgtgcg tgatgtggtg cgcgcgtact ggctcgcgct cgaccgggcg ccggccggca
     7981 cctacaacgt ctgctccggt gctggcacgc aggtggtcga tatcctcgcc gcgctcgtcg
     8041 ggcagacgtc gctgcgcgtt gagcaacaaa ccgacccggc gctggttagg gaacacgaag
     8101 tcatggagat ccggggctcg aacgacaagc tgcagggggc gacgggctgg cgccccgagc
     8161 tcgcgctcga gcgcacgctg cgcgacacgc tcgactggtg gagggagcgc ctcggcgctc
     8221 cggtgccgcg atgagccgca cggccctcat caccggcatc accggccagg acggcagcta
     8281 cctggccgac ctgctgctcg agaagggcta cgaggtgcac ggcatggtgc gccgcgcttc
     8341 gagcgagacg ttccagcggc tcgcccacat ccgcgacagc atcacgctgc accagggcga
     8401 tctgctcgac cagagctcgc tcaccgacgt gctgcgcgcc tgcgagcccg acgagatcta
     8461 caacctcgcc gcgatgtcct acgtgcccac gtcctggacc cagccggtcc tgacggcgga
     8521 gttcagcggc acgggcgtca cgcgcatcct cgaggcgatg cgcgcggcgg cgccggacgc
     8581 gcgcttctac caggcgtcct cctcggagat gttcgggaag gtgcgcgagg tcccgcagac
     8641 cgagatgacc ccgttctacc cgcgcagccc gtacggcgtg gcgaaggcgt acggccactt
     8701 catcacggtg aactaccgcg agagctacga cctgcactgc tcctcgggga tcctgttcaa
     8761 ccacgagagc ccgcggcgcg gcctcgagtt cgtcacgcgg aaggtcaccc acggcgtcgc
     8821 ggcgatcaag ctcgggatgg ccgatcacct ggcgctcggg aacctcgacg ccgagcgcga
     8881 ctggggctac gcgaaggact acgtcgaggc gatgtggatg atgctccagc aggacgagcc
     8941 ggacgactac gtgatcgcga ccggcacgac ctacagcgtc cgccagctcg tcgagctctc
     9001 gttcgagcac gtcgggctcg accccgacga ctacgtgcgc atggacccgc gcttcctgcg
     9061 gccggccgag gtcgaccacc tgatcggcga cccggcgaag gcgcgcgaga agctcggctg
     9121 ggagccgcgc acgagcttcc cggagctcgt caagctgatg gtcgacgccg acctcgagct
     9181 cctcgcggcc ggcgtgcccc agcagcaggc aggctgacgc catgcgcacg gagctgctgg
     9241 agcagctgcg ctgcccgcgc tgccaggtgg agcggtcgtt cgacgtcacg gtcgccgagg
     9301 aggacgagcg cgagatccgc gccggcgagc tcacctgccg ggcgtgcaac cacaccgcga
     9361 cgatcgagga cgggatcgtc gacctgctct tcgagccccc ggagttcgtg acccgcgagg
     9421 ccgccgggct cgggcgcttc gtcgaccaga tggtcgccga cgggtgggac cgcgagcgga
     9481 tcctcaagct cccgtacgag cagtccggct actggttctc gcaggcggcc gcgatggagc
     9541 acctgctcga gatcaacccg ccgcggcccg gcgcgacgct gctcgacatc ggctcgaaca
     9601 cgtgctgggc gagcaacatc ttcgccaagc gcggcctgcg cgtgaccgcg ctcgacatct
     9661 ccactcccca catgcagggg ctgaagaccg ccgactggtg gatggaggag aacgacatct
     9721 acttcgagcg gatcctctcg gtgatgttcg acccggcgct ggccaccggc tcctacgact
     9781 acgtcttctg ctgcgaggtc ctccaccaca accacaggga gaacctccag cgcacgctgc
     9841 gcgagctgta ccggatcctc aagcccgacg gcaagctgat cgtgctgaac gagccgctca
     9901 agttcccgac gaaccgcaag gaggaccacg cccaggaggt cgccgagttc gaggggcacg
     9961 agcacgtcta ctacatgcac gagtacctcc tggccgcgta ccgggccggg ttcaagccga
    10021 gcatcgtgcg cccgaacacc acgccgttct tccgcgggga ggaggtcgtg ctcaggcagc
    10081 aggaaccgat gaaggcatcc gcccggaaga tggcgaggtt ctggatccgg aagctgccgc
    10141 gtgcgcgccg cgcctacacg gcgtacaagc tcgccatcgg gcccgacgac ctctcgctgg
    10201 ggatgctctg cacgaagcgg gcgagcggag ggtgagcccc gcgggtcgga cggtcagcgt
    10261 cgcgatcccg gtgcgcaacg gcgggaggag cttcgtggcg ctgctggatg cgctcggcgc
    10321 gcagcggatc gatcgcgagg tggaggtcgt ggtggccgac aacgagtcca ccgacggcac
    10381 gcgcgaggag gccgcgcggc gcggggccac gatcgtcgac gtgccgcagg gggagttcag
    10441 ccacggcggc acgcgcgacc tgctgatgga gcgcgcccgc ggcgagcacg tcgcgttcgt
    10501 gaccgacgac gtgctgccgg tcggcgactc ctggctggcc gagctggtgg ccggcttcga
    10561 cgatgccgag cgcgtcggcc tcgtgtacgg gccatacgtg ccgcgccccg acgccagcca
    10621 catggtcagc cgcgagctca ccgagttctt cctctccctc tcgcccgacg ggaccccgcg
    10681 cgtcgaccgc gggctgcgtc ccgaggacgt gcggcccggc cccgtcacct tctacaccga
    10741 cgccaacggc tgcgtcgcgc gcgcggcctg ggagcgggtg cgcttccggc cggtgcgcta
    10801 cgcggaggac cagctgctcg cgctcgacat gctgcgcgcc ggcttcgcga aggtctaccg
    10861 gccgcgggcg gccgtcatcc actcgcacga ctacccgttg cgcgagcgct ttggccgttt
    10921 cttcgatgag ttccgcgcgc tgcgcgaggt ctacggtcac gtcgaggagg ccggctggtc
    10981 gtacaccccg gccgtcgtgc gggcgggcgt gcgccgcgac cgcggcttcc tgcgcgcgca
    11041 ggggcgcagc ggctgggagg tagaccgcgg cacgctcgag tcgctcgtct accacgggat
    11101 ccgcgcgacc gcggccatcc tcggcacccg cgcggaccgc ctcccgccgc gggtccggcg
    11161 cgccctctcg cgggagcgcc gcgaccagtt cgagccgctc gaacggggcc tcgtctaggc
    11221 gcgtttgcgc ctcctaccct tgcgcccgat gccgaccgag accgccatat gagcccccgc
    11281 cggcgccgcc tggaggcgct gcgactgcgc atggtcgacg tgttcggcaa cctgcccaag
    11341 cgcgcgtact tgacgctgcg ctaccgcggc gtcgggtcgt tcctgttccg ggcgatcacg
    11401 ttcccgctgc ggctcacccc ggcggcgcgc ttcctgcccg tcgggcggga ccggacctac
    11461 gccgccgcgg cgcgccgctg gtacgagctg aacgggcggc cggtgaccat cgcgatcccg
    11521 cactggggcc cgaccgacca gaccgtcgag accgtgcagg cgctgctcgg gacgaccgac
    11581 cccgagcgcg tgcgcatcgt ggtggccgac gacgcgagcc ccgcgccgta ccccgaggag
    11641 ctgcgccgcc gcctggccgg catggccgag gtgatcctcg gcgaggagaa cggcggcttc
    11701 tcggtcaacg ccaaccgcgc gattcgcgcg gcccggccgg gcgacgacgt cgtgctcctg
    11761 aacagcgacg tgaccgcgct gccgggctgg ctcgaggcgc tccagtacgc ggcgtacggg
    11821 tccgacgaca tcgggctcgt cgggccgaag ctgctctacc cggaccgcac gatccagtcg
    11881 gccggctcgc accgcaacca ggccgacccg atctggttcg accaccgcta ccgcttcaag
    11941 ccgctcgacc acccgccggc ggagttccag gccccgtgca tcgcgatgac cggcgcctgc
    12001 atctacgcca agcgcaccac gatcgacacg atcgggctgc tcgacgagcg ctacccgatg
    12061 gcctacgagg acgtcgactg gtgcctgcgc gcgtggcagg cggggctgcg cgtgatctac
    12121 cagccgttct cggcgatgtt ccacctcgag tcgaagaccc gcgggaccga gcagggcgag
    12181 cgcgagctga agtcgcagcg gctgttctgg gaggagtggg gcgactggtt cgaccgccgc
    12241 caggtgcgaa cgccggaggg caagctgcgg atcgtctacg tcaccgagga cacgggcgtc
    12301 ggcggcggcc accgggtcgt gttcgagcac ctgaacgggc tggcggcgcg cggtcacgag
    12361 tgcgagctgt ggtcgctcga cacggcgccc gggtggttcg acctcgacgt gccggtccgc
    12421 acgttcgagg actacgacgc gctgacggag gccctcgagc cgctcgaggc catcaaggtg
    12481 gcgacgtggt ggaacaccgc cgcaccggtc tggcgcgcgt cggtggtcta cggcctgccg
    12541 gtctacttcg tccaggacat cgagacctcg tactacccca accatccgca catgcacggg
    12601 tcggtgatga acagctaccg ccaggagttc cgctacctga cgacctcgga ctgggtggcc
    12661 ggcaagctcc gcgagctgca cctcgacccg cacatcgtgc cgccgggcgt ggacctcgag
    12721 accttccacg agctcgacgt cgagcgcgag gacaacgtcg tgctcacgct cgggcgcagc
    12781 aacccgctga agaacctcga cctcacgctc gacgcctgga gctcgatgcc caacggacgc
    12841 cccgagctgt ggatgttcgg catcgagccg gagctgggcg agcggcccgg cgcgcgctac
    12901 ttcgtgcggc cgagcgacgc cgaggtgaac gacctgatga accgcaccac cgcgctcgtc
    12961 cagacctcgc gccacgaggg gttctgcctg ccgctgctcg aggccatggc cgccggtgcg
    13021 ccagttgtct gcacggacgc tcacgggaac cgtgatttct gccgtgacgg ggataattgc
    13081 ctcatggtcc acgacgaccc ggcgtcggtg cgcgccgcgc tggagcgcct catgcgcgat
    13141 cgccagttgc gcgagcgcct gtcggcggag ggtcgccgga ccgccgagcg ctacgcctgg
    13201 cctggtctga tcgaccaggt ggagcggttc ttcgaggacg tggcgccgga ccccgtttga
    13261 gggtcggcgt cctgatacac gagttcggtc gctcgggcgg cgcgcagacc gtcctccgct
    13321 acgcccgcgc gctggaggac gcggagctgc tggtcacggc gcccagggcg cgcgaccttc
    13381 ccgcggaggt cgacggcgtg cccgtccgcc acgtgagcga ggccgggggc cggcgctacg
    13441 actgcgcgat cgccacctgg tggaccaccg ccgagtcgct ctgggaggtc gaggccgacc
    13501 ggcggctggt cttcctccag agcatcgagc aacgcttcta cgaggagcac cactggtacg
    13561 agcgcttcgc ggccgaggag gtgctggcgc tgccggtcgg ctacgtggtc gtcgcgccgt
    13621 ggatgcgcga cctgctggcc gagctgcgcc cggacgctcc gtgcgtcgtg gtccgcaacg
    13681 ggatcgacaa ggagatgttc cggccacgcg gcggcggcgg cgaccgggcg gacggtccgc
    13741 tccgcgtgct cgtcgagggt cagccgacga tgtggttcaa gggcgtgcgg gagtcgctcg
    13801 ccgcggtgcg ggcgatgcgc gagccggtcg aggcgacgct ggtcgccggc gacccggact
    13861 cggccggcgg cgtggacggg gttcgcgtcg tcggcggcct cgacccggcc ggcatggccg
    13921 aggtctacgc cgcgcacgac gtggtgctga agctctcgcg tgtcgagagc ctgggccttg
    13981 gcccgatcga gggcttccac gtcgggctgc cggcggtcgt gacgccgtac accgggcacg
    14041 aggagtacct cgaccacgat cgcaacgggc tcgtcgtggg cttcgacgac gtgccaggca
    14101 ccgcccgcgc gctcgactcg ctcgcggccg accgcggacg cctgcggcgc ctggcggagg
    14161 gggcgctcgc gacggcggcg tcgtggccgg cggcggacgc cgcggcgagc gcgttcgagg
    14221 acgcgatccg cgagttgtgc ggcgggccgc cgcccgacgc cgacggagcg ctcacgcagc
    14281 ttcagcgcgc gcagcggcgc tggctcgagc tgagccgcga gcacgcccgc caggagctca
    14341 cggtcgccga gggactgcgc ggctcggtcc ggtggtacga gcatgcgctc gccgaggcgg
    14401 cggagcatca tcgcgagctc gaccgccagc gccacgaggt gagcgagcgg gcgagcgtgt
    14461 tcgaggcgga gctcgcgcgg cggaccgagc agctcgagga gctgaaggcg acgcgcgcct
    14521 accgcgcggc ggccgcgctg cgacgactga agcccggtcg cgacgcatga ccccgctgcg
    14581 cgtcctgggc gacgaccgcg ccctggccct ggcgggcccg cccggcgccg agcccgcggc
    14641 cgggcccgcg gtccacctcg gcgagccctc caccgagccc gcccccggcg acgtggtgtg
    14701 gctccccggc cccgcctccg agcccccgtg cgcgggcgtg cggctcgtgg ccacgggcgg
    14761 cgacgcactc tggtcgcgcg cgccgtggcc cgcccgcgac gagctcttcg acctcgctct
    14821 ccccaccgac gagtgcgcgc tcgtggtcgg cgccgacgac gcgcggcgcg cggtcgtggt
    14881 cgagaagctc gaggcgcgcg ggcgcccggt cctcgtggcg ccgcaactga gcgccggcga
    14941 cctcgcgcgc gtgtccgtcg tggcgctgct cggcgacgcc gacgcccccg cgccggcgag
    15001 tccgtggtcg gcccgcgcga tgcccgccga ggccccggcc gtgctcgccg cgcggcgggt
    15061 cctcgtggcg ccgcgctgcg gcctcacgtt cgggctcctg cccggcagcg accacctcgc
    15121 cttcggcgcc gaggacgacg tcgtctccta catcgacgcg gtgctcacct tccctcaggc
    15181 gttcgagccg ctccccgcac tgggcgcgct cgccgccgag cgccaccgcg cgtcgcgcgt
    15241 gtacgcgcgc ctcgccctcg agctgcgcgc cgccgccgac cccgccgccg ccgacgaccc
    15301 cgccgccgcc gaccccgccg ccgccgaccc cgccggctag atctgctccg cgatgtgcgg
    15361 ggcctcgcgg ttgaacagcc agaagcccag caccgtggtg agcaccatga tcgctatcgg
    15421 gatcagcagc ccggtcgggc cggccgcggc ggccgcggtc ggcgcgctcg ggtcgatcac
    15481 cgcgtggcgc gtctgctcga ggaacaccga gaccgggttg agcatcacga gctcgcccca
    15541 ctcgggcttc gggatcgcct cgatgggata gatcaccggc gccgcgtaga acatcgcctg
    15601 tgagatgacc tgccagatcg gctgcacgtc ccgcgcgcgc acgtacagcg ccgagagcag
    15661 catcgacagg ccgaccgccc aggtcccgag cgcgagcatc agcaccggga actcgagcca
    15721 cgaccagcgc ggctcgacgc cctgcgcgag ggcgaagacg cccacggccg ccaggttgat
    15781 cgtcaggttg aggaacgccg tcagcaccac ggccagcggg atcaccatgc gcgggaagtg
    15841 gatcttgcgg accaggttct cgcgatcgac gagcgagctg atcgccgcgg cggtggagtc
    15901 ggtgaagaac gtgaagatca cgatgctcat cagcagcacc accgcgaagt gctccgcctg
    15961 ctgcccgatc cgcacgaact ccgtgaacac gaagtagagg accgtgaaca gcatcagcgg
    16021 ccgcatgagc tgccacaggt agccgaggaa cgacccgaag aaggtcagct tgaagtcggt
    16081 gatcgccagc gtccaggtga ggtgcaggaa cctgcgccag tcgccgccga gcgccgacgg
    16141 cccgcggatc cgcttcccga atgcgctctc ggcggtgctc acgtggcccg cgcggtcagc
    16201 ttctcgccgg acggcgtctc gatcgtcacg tcgtggtcga agttcacgag cccgccggtc
    16261 gcgtgcgcgc cggtgctcac cacggtcgct agttgcgggc gccggtcgag caggtggttg
    16321 ccgggcggcc cggtcgtgac ccacggcgac gcgtacagcc gccccggcgc gaacacgttc
    16381 tcgaactgca ccgagaacac gacctcgtca ccggcggagt agctgccggt gccccagtcg
    16441 gccgcgtcgg tggacgtggc gaacagcggc aggtgggcct cgttctcgag caggactgca
    16501 agcgcggggt tcacgatctc ccgctggaac acgacgcgca tgcgaaagct gcacggctcg
    16561 ccctggggga gcgcggtctg gcgctcgccg tcgtcgtcct cgaaccaggc ctcggcgatc
    16621 tgcgcggtct ggtcgccgta gcgctcctcg ccgctctcct cgtcgcgacc cccctcgcgc
    16681 ccgaagttca gctcgaggta gttgctcgcc acctcgtcgg gcggtccgat catccgcatg
    16741 ttgccgcgct cgataagcag cgcgcggtcg cagaagcgct tgacggctcc catgtcgtgg
    16801 gtcacgaaca ggatcgtccg gccctcctcg cgcatccggt ggaagacgtc gaagcacttc
    16861 tgctggaacg aggcgtcgcc caccgccagc acctcgtcga tcagcaggat gtcggcgtcg
    16921 acctcgatca tcaccgagaa cgcgagccgc acctgcatcc cggacgagta gttcttgagc
    16981 ttgaggtcca cgaactcgtg cagctcggcg aagtcgatca cgcgctcgaa gcgctcgcgc
    17041 gcgtccttcg gcgagagccc gagcatgacc gcgttgagcg ccacgttgtc gcgggcggcg
    17101 aggtccgggt tgaagcccac tcccagctcg atgaaggtcg acacgcgacc gttcacgtag
    17161 atcgcgccgg tgtcgacgcc atagatgccc gccatgcact tgaggagcgt gctcttgccg
    17221 gacccgttgc gccccacgat cccgaagaac tcgcccttcg gcaccgcgaa cgacacgtcc
    17281 ttgagcgcct cgaactggtc gtgaccggtg cgcctgaacg gatggagcgc gcgctccttg
    17341 aacgtgtgga cggcctcgtg tggcttccgg aacgtcttcg atacgccgtc gacgacgacc
    17401 gccggcagct cgctgtcggg cggcgggctc atcggggcaa atgtagctcc gcacgctcgg
    17461 ccaccagccg ggcgatggcg aatgcggagg tggccgcggg ggagggggcg ttgcgaacgt
    17521 gaagggcgcg ttcggtctcg gagagcacga agtcgtcgag cagccggccg tcgcggccca
    17581 ccgcctgtgc gcggacgccg gacggcccgg ggagcacgtc cgcgggcccc agctcgggca
    17641 cgaagcgcgc cgccgcgcgc acgagcgagc gccggctgcc cgcgtggcgc agctcgctca
    17701 gaccggtccg ccagaagcgg cccatcagcc gccaggtgcc gccgaagcgc aggccgccaa
    17761 cgagaagcgc ggtcgggccg atcgtcacct cgccgtcgac gtgtcgcgtg aggtgcacgc
    17821 cgaggaacgg cagccgcggg tcgggcaccg ggtagatcag cgagcggacg agctcgcggc
    17881 gctcggggcg cagccgcaga tagccgccgc ggaacgggac gacatgtggg tcgcgcggac
    17941 cgctggcggt ccgcgcgagc cggtccgacc acagcccggc gcagaagatc gcgtggcgcg
    18001 cgcgcagctc gccgcgggag tggcgcagca cgagccggcg gccacccgcc gcgactcccg
    18061 tgaccgcgca gccggtgtgc acaggcacgc cgtcgcgctc gtgatcgag
//
LOCUS       DASVPL010000115         4351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_53601, whole genome shotgun sequence.
ACCESSION   DASVPL010000115 DASVPL010000000
VERSION     DASVPL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53601"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VF517_05790"
     CDS             <1..146
                     /locus_tag="VF517_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX8052485.1"
                     /translation="GLAYRSHHHRNRALPDWLAYYNERRPHSSIGGLAPISRVHNVRG
                     QDN"
     gene            complement(269..739)
                     /gene="rpsG"
                     /locus_tag="VF517_05795"
     CDS             complement(269..739)
                     /gene="rpsG"
                     /locus_tag="VF517_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX8052486.1"
                     /translation="MPRRNSPGIRAIEADPVHGSRLVQQIVNKVMIDGKKSTAEKIVY
                     DALETLSRRTGQPPVEALEASVKALTPVLEVRSRRVGGATYQVPVEVPQRRARTLAVR
                     WLVSFARDRREKSMADRLAGELADAQSDQGGAFKRKEDIYRMAQANKAFAHYRW"
     gene            complement(743..1138)
                     /gene="rpsL"
                     /locus_tag="VF517_05800"
     CDS             complement(743..1138)
                     /gene="rpsL"
                     /locus_tag="VF517_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX8052487.1"
                     /translation="MPSTNQLVRKGRRKPKKKVATPGLKSGQGRKKKIAAPQRRGVCT
                     RVYTTTPKKPNSALRKVARVRLTNGMEVTTYIPGEGHNLQEHSVVLVRGGRVKDLPGV
                     RYKVVRGTLDAAGVSDRKKARSQYGVKAK"
     gene            complement(1174..2046)
                     /locus_tag="VF517_05805"
     CDS             complement(1174..2046)
                     /locus_tag="VF517_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8052488.1"
                     /translation="MSVQLGIALAVLCALTTNLAFLYKHRGARRAPDVEWRHPWKSGV
                     ALFRSRWFAIGMLVALGAWVLHVAALALAPLSTVQAVICGGLVMLAVLAERVFGFKVG
                     KRQWIGVAFTALGLALLAITLPAAGDANSNYSTAGMIIFETGLLGAGILLVLFPKLGA
                     PHHHHGVVFGTAAGILFGVSDVAIKALTGAVAAHGAMGLASPWLLTCIAASIIAFFAS
                     ARGLQKGDAVPVITTTSAAANVTAIAGGISVFGDPLPGDPLGVVVQIFAFVLVIGAAA
                     LTPGPLRAAGASAH"
     gene            2204..2716
                     /locus_tag="VF517_05810"
     CDS             2204..2716
                     /locus_tag="VF517_05810"
                     /inference="COORDINATES: protein motif:HMM:NF037735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HEX8052489.1"
                     /translation="MSNVPHMRPILFVDVDGVLSLYGWHRDGDPPGSFHLVDGILHCI
                     GSSCAPLLARLRDRFELVWATGWEEKANEYLVHLLELEADLPVLSFDGRARFGSAHWK
                     LDALEEFAGDRPAAWIDDNIDAECRTWAERRSAPTLLVQTETHTGITAEHVDELLRWA
                     DAVTRQAPTS"
     gene            complement(2698..4209)
                     /locus_tag="VF517_05815"
     CDS             complement(2698..4209)
                     /locus_tag="VF517_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052490.1"
                     /translation="MGRVTARMTRGEWLLLGLLAAWSLIPLAQGILHLMQNGGVLGGT
                     DGIGVVDHFQYLSWIRESGEHGLASNRYDLADSDAVYLQPMWLVSGLAWAAGLPIQAG
                     LLLWKPVCVAVLFGGSLAYVRRLVEGRGARLAALAIALFYFPPLAVLLNRTGLGSDAA
                     DGLAYLFGFQLSPASFLWGYVQTAVAVGLMPLFLLALERRRIAWAAAAGLLVSWVHPW
                     QGMVLLGVLLGVAVWTRSVRSLVLPALATLAPLVYFLVLARVDPAWGEGSESGGLAHY
                     WGDLLLAVGPLAAFAAWGAWAGRGRELDLQERVLLLWPLVTLLVYAVLDRTFVYNVLS
                     GLTIPLAILAVRGAARLPRGVAVAAVLVATAPGLVNLVVELRDGLGAGNSPRYLAAGE
                     ADALEFVDESPREGGVLTRLYLGQAVPGLSGRRTYVGHPSWTPDIARRVKETEDLFAG
                     AATPGQARALVRRTGAAFVVQDCARRHDLTAELGPLLRSTHRFGCATVYEVGA"
BASE COUNT          686 a         1578 c         1544 g          543 t
ORIGIN      
        1 acgggctcgc ctaccgctcc caccaccacc gcaaccgcgc cctgccagac tggctcgcct
       61 actacaacga gcgcaggccg cacagctcga tcggagggct agcgccgatc agccgcgttc
      121 acaacgtccg tgggcaggac aactagctac cggtacaggt ggtggcgatg ccgcgaaaag
      181 cgaggatgcg aaggacgcag ggagcggaca gtgcaccgca cgtccgcgac cgaggacgca
      241 cgcagccgac gctttgcagc gagcagcgtc accacctgta gtgggcgaag gccttgttgg
      301 cctgagccat ccggtagatg tcctccttgc gcttgaacgc gccgccctgg tcggactgcg
      361 cgtccgcgag ctcgccggcc aggcggtcgg ccatgctctt ctcgcggcgg tcgcgcgcga
      421 agctcacgag ccagcgcacc gcgagcgtgc gcgcgcggcg ctggggcacc tcgacgggca
      481 cctggtaggt cgcgccgccg acgcggcggg agcggacctc gaggacgggg gtgagagcct
      541 tcacggacgc ctcgagcgcc tcgaccggcg gctggccggt gcggcgcgag agcgtctcga
      601 gcgcgtcgta gacgatcttc tcggcggtgg acttcttgcc gtcgatcatc accttgttga
      661 cgatctgctg gacgagccgc gagccgtgga ccggatcggc ctcgatcgca cgaatgcccg
      721 gtgagttgcg gcgcggcacg ggctacttcg ccttgacgcc gtactgcgag cgggccttct
      781 tgcggtcgga gacgccggcg gcgtcgagcg tcccgcggac gaccttgtag cgcacgcccg
      841 gaaggtcctt gacgcggccg ccgcggacga gcacgaccga gtgctcctgg aggttgtggc
      901 cctcgcccgg gatgtaggtc gtgacctcca tgccgttggt cagccgcacg cgcgcgacct
      961 tgcgcagcgc ggagttcggc ttcttcggcg tggtggtgta cacgcgcgtg cacacgcccc
     1021 gccgctgcgg cgcggcgatc ttcttcttgc gcccctggcc ggacttcaga cccggggtgg
     1081 cgaccttctt cttgggcttg cgccgcccct tgcggacaag ctgattcgtg ctcggcatac
     1141 ggcgcgcgat gctagcaggg gagcggcgcc gcgctagtgc gcgctggcgc ccgcggcacg
     1201 cagcgggccg ggggtcaggg cggctgcccc gatcaccaga acgaaggcga agatctgcac
     1261 gaccacgccg agcgggtcgc ccgggagggg atcgccgaag accgagatcc cgcccgcgat
     1321 ggcggtcacg ttcgccgcgg cgctcgtggt ggtgatgacc ggcacggcgt cgcccttctg
     1381 gaggccgcgc gcggaggcga agaaggcgat gatcgaagcg gcgatgcagg tcaacagcca
     1441 cggggacgcg aggcccatcg ctccgtgcgc ggcgaccgcg cccgtgagcg ccttgatcgc
     1501 cacgtcggag acgccgaaga ggatgccggc ggcggtcccg aagaccacgc cgtggtggtg
     1561 gtgcggcgcg ccgagcttgg ggaacagcac gagcaggatg cccgcgccga gcaggccggt
     1621 ctcgaagatg atcatgccgg cggtcgagta gttcgagtta gcgtcgcccg cggccggcag
     1681 ggtgatggcc agcagcgcca ggccgagggc ggtgaacgcc acgccgatcc actgccgctt
     1741 gccgaccttg aagccgaaga cgcgctcggc cagcacggcc agcatcacga gcccgccgca
     1801 gatgaccgcc tggacggtcg acagcggcgc gagcgccagc gcggccacgt ggagcaccca
     1861 cgcgccgagc gcgaccagca tgccgatcgc gaaccagcgc gagcggaaca gcgccactcc
     1921 gctcttccac ggatggcgcc actcgacgtc cggagcccgg cgcgcacccc tgtgcttgta
     1981 gaggaacgcg aggttcgtcg tcagggcgca gagaactgcg agggctatgc ctagttggac
     2041 gctcatcgaa agtttgtacg gcggtgaccc ggccggtccc cgcgactagt tcgtgctatc
     2101 ggcgcgagtg cctgcccgct tgagacttca cactcagggg cttagacccc ttgtctacct
     2161 aaggttgcgg cccggcaaac ctctcccccc cgacccttaa ctcgtgagca acgttccgca
     2221 catgcgcccg atcctcttcg tggacgtcga cggggtgctc agcctgtacg gctggcaccg
     2281 cgacggcgac cccccgggct ccttccacct ggtcgatggg atcctccact gcatcggctc
     2341 ctcctgcgcc ccgctcctcg cgcgcctgcg cgaccggttc gagctcgtct gggccaccgg
     2401 ctgggaggag aaggccaacg agtacctggt ccacctgctc gagctggagg cggacctgcc
     2461 cgtgctgagc ttcgacggcc gcgcccgctt cggcagcgcg cactggaagc tcgacgcgct
     2521 cgaggagttc gcgggcgatc gccccgccgc ctggatcgat gacaacatcg acgccgagtg
     2581 ccgcacctgg gccgagcgcc gctcggcgcc cacgctgctc gtccagaccg agacgcacac
     2641 cggcatcacc gccgagcacg tggacgagct gctgcgctgg gccgacgcgg tgacgcgtca
     2701 ggccccgacc tcgtagacgg tcgcgcagcc gaaccggtgc gtgctgcgca gcagcggccc
     2761 gagctccgcg gtcaggtcgt gacgcctcgc gcagtcctgc accacgaacg ccgcgccggt
     2821 gcggcgcacg agcgcgcggg cctgcccggg agtcgcggcc cccgcgaaca ggtcctcggt
     2881 ctccttaacg cgccgggcta tgtccggcgt ccacgacggg tggccgacgt aggtgcgccg
     2941 gccgctcagc ccgggcaccg cctggcccag gtacaggcgc gtgagcacgc cgccctcgcg
     3001 cggcgactcg tccacgaact cgagcgcgtc cgcctcgccc gccgcgaggt agcgcggcga
     3061 gttgccggcc ccgagtccgt cgcgcagctc gaccaccagg ttcacgagcc cgggcgccgt
     3121 ggcgaccagg accgccgcga ccgcgacccc gcgcggcagg cgggccgccc cgcgcacggc
     3181 gaggatcgcc agcgggatgg tgaggcccga cagcacgttg tagacgaagg tcctgtcgag
     3241 caccgcgtag acgaggagcg tgacgagcgg ccagagcagc agcacgcgct cctggaggtc
     3301 gagctcccgt ccgcggccgg cccacgcgcc ccacgccgcg aaggcggcca ggggaccgac
     3361 cgcgaggagg aggtcgcccc agtagtgcgc gaggccgccc gactcgctgc cctcacccca
     3421 ggccgggtcg acccgcgcga gcacgaggaa gtacacgagc ggcgcgagcg tggcgagggc
     3481 cgggagcacg agcgaacgca cgcttcgcgt ccacaccgcc acgcccagca ggaccccgag
     3541 cagcaccatc ccctgccacg ggtgcaccca ggagacgagc aggcccgcgg ccgcggccca
     3601 cgcgatgcgc cggcgctcca gcgccaggag gaagagcggc atcagtccga ccgccaccgc
     3661 ggtctgcacg tagccccaca ggaacgaggc cggggagagc tggaagccga acaggtacgc
     3721 gagcccgtcg gcggcgtcgg agccgagccc ggtgcggttg agcagcacgg cgagcggggg
     3781 gaagtagaac agggcgatcg cgagcgccgc cagccgtgcc ccgcgcccct cgaccagccg
     3841 ccgcacgtac gcgagcgagc cgccgaacag caccgccacg cacacgggct tccagagcag
     3901 cagccccgcc tggatcggca ggccagcggc ccatgcaagc ccggacacga gccacatcgg
     3961 ctggaggtag accgcgtcgg aatcggccag gtcgtaccgg ttcgaggcca gcccgtgctc
     4021 gccggactcg cggatccacg acaggtactg gaagtggtcg acgaccccga tgccgtcggt
     4081 gccgccgagc accccgccgt tctgcatcag gtggaggatc ccctgggcga gggggatcag
     4141 cgaccacgcg gcgagcagcc ccagcaggag ccactcgccg cgtgtcattc gagcggtgac
     4201 gcggcccacg ggccgcacgc tagctgagcc gcccacacac cttgtgaacg gctgaacgac
     4261 gtccgggcct ctccagaggc tgtgtgtctg cgacgactca ccgcccaagg agggcccgga
     4321 tgcacactca cgctagagcc aagctcggac c
//
LOCUS       DASVPL010000116        13998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_250567, whole genome shotgun sequence.
ACCESSION   DASVPL010000116 DASVPL010000000
VERSION     DASVPL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13998
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250567"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..554
                     /locus_tag="VF517_05820"
     CDS             120..554
                     /locus_tag="VF517_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEX8052491.1"
                     /translation="MRGAMHEATVLYDRDCGFCRWTLAQILAWDRAKRLRPVPIQSEE
                     GQRLLARVPEAERLESWHLVTPDGAVKSAAEGFPGLFSRLPAGAPIARLTALLQGPSS
                     VGYRLLVANRTRLGRRISQERRDRATEALDAHERAARAAAAA"
     gene            586..1149
                     /locus_tag="VF517_05825"
     CDS             586..1149
                     /locus_tag="VF517_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052492.1"
                     /translation="MGPELPEGEPQPQQPAAPVLDEASVQRRVFVVRMAAWGTVLLLA
                     VIAFNLRAGASADTDQGANGPQVNGRTSQGQAIWSIADREQVREIQMSWQFKCEGGGR
                     LEPFGGTFRDSTDHFDYDGAKFKVAIEDEMPQGKDGWTAHLKAEVGGEVHADGQAVGT
                     SAAVMWFTRTGQRGAVCRSGPVRWSTP"
     gene            complement(1146..1532)
                     /locus_tag="VF517_05830"
     CDS             complement(1146..1532)
                     /locus_tag="VF517_05830"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX8052493.1"
                     /translation="MDSRPRWLVDGMNVVGSRPDGWWRDRRAAMRRLITQLETFAAAT
                     GDTVAVVFDGHPFGLDGGGLVEVGFAPGGRNAADHAIADWVRTDPDPGGLTVVTSDHE
                     LEEAVRAAGAKVVGSGAFRRRLDEGG"
     gene            1578..2417
                     /locus_tag="VF517_05835"
     CDS             1578..2417
                     /locus_tag="VF517_05835"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8052494.1"
                     /translation="MAATQAEVVAAWQLRQAGWSATKIRHHAARGAWRRVHPGVYVLT
                     RAQPSQVQLWWAAALTTPTSFLSHGSAAACYGFHRFSKPFEVVTRPGQGGRRRLGRLL
                     VFRSKRLQGETTRHTGIPITTAERTLVDVAAGLDAKRVGRCFREAIRLKTTTAARVAR
                     CVERHGDAPTLLAAVARRYAKLPYRRTRSDAEARALELLDDAGIRPPGVNVRIGGEEA
                     DLVWVERRLIVEIDGPQFHLFRDEDARKEAIWRDAGFTVRRVPSSTVYDDPAAFVACC
                     VRP"
     gene            complement(2407..4743)
                     /locus_tag="VF517_05840"
     CDS             complement(2407..4743)
                     /locus_tag="VF517_05840"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX8052495.1"
                     /translation="MRIALVSPYSFSYPGGVGRHAEALADELLARGHDVRLLAPFDPD
                     DRVARLTHRGGRPATRELPDHFVSLGRTYGFSMNGSQSNISISADAVATLNRTLRHGD
                     FDVVHVHEPNTPVLSWVATENTRAPLVGTFHTYSTNTPVHLFTANVLNARRVYSKLHE
                     RIAVSEAARWTAQRFYGGNYRVIPNGVDLSAAPTGPKPEGRELRLLFVGRAEERKGLP
                     VLLRAFEALREAGVRTRLTVAGATPEEVEPYLLDPEGVHVAGRVTEEEKWRLLHEADV
                     VCAPSLGGESFGMVLTEAFAAGTPVVASDIAGYRDVVRDGVDGVLVPAGHPAELGEAL
                     LALATDPERRARMGAAARAGAQRFSWPHVTDQVMESYERAIERAAVVPEGRAARVALR
                     TGFAPADGLPRNPPRRIPSVEPELPGARRRRAVRVARRVGIAAAGGAAIGLAGLAFQR
                     IGWESIGQSMIAATPWWVFVAFALMCSSMLLRAESWHAILRAALPGLKVRRRDAARGV
                     MIGVLMSATLPARLGEPSRALIVARRLGRVRERLPTVLGTLVSQTILNLFALAVLGSI
                     MFMTVGLFEGRERSLLWVAIAPVVALILVALAPFLLRKGKPSRFARVQQLASTVRRAM
                     VDARRGLRVFRRPKLGAWAAATQLGAWAIQWVACYTLLIALGLDDRAGMGAAAAVLFA
                     VNVTAAIPATPSNIGVFQAACVAVLSAYGVGHADAFAYGIILQAVEIATAFTLGMPAL
                     VREGMSWRDLRLRALHAAPVELSATRQRREAEAARSRA"
     gene            complement(4753..6750)
                     /locus_tag="VF517_05845"
     CDS             complement(4753..6750)
                     /locus_tag="VF517_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052496.1"
                     /translation="MRRRAAFLAILGAFGLLCALPAAAAAAPRALIAFVPTEPAPKSP
                     LLFDLAQRDFSYGMASPSIGAYSKRQMVLDMSQGSRIANSAYPDKLKRLDLRLDPDGG
                     GQVKGFRRARARADDAPGDVVPGLLGDTLRRRGASAAYVGVVGFPQTEAVVAADRQGR
                     VERVSLGTIGTFADRTLELWSDHEVVVARFPSDEAGLAALDRVVAARGPDDLILVVRA
                     PPAGRGRLLPAGMLGPGAEQQVIFSPTTRRLGLVAVTDYATTVLDHLGIGVPDAMDGR
                     TIDPRPDGDPEEVRLRMARLDVVLSRRGHGLRWFVIGFAGLATALWFRRRREGLDEAL
                     RTGFLGAMWLPGVALLTAYLQPTRLAETILLAGGSLALGRATDRLVRWPLAPALPGLA
                     VFAAHAIDLARGSPLIGASLAGPNPKGGARFFGIGNELEILLSLEVLFGLAALLTLFA
                     GRRVPIGFALGCLVAAVTIGAGRLGADVGGVITLGAGAAGAVLASLPGGPSRRAVALA
                     VMAPAVAVVGLIGIDLLTGGGAHLTRTVLEGDGASGLAEIVKRRLLISANGLDTVKMI
                     VLVATGIVCMIWGVRRRQDLLRPLRPHPALVAGLWGALAATVIGALSNDSGPVIFVAG
                     FFGLLLALGYGRGAPGAAPRRAARRDAQGAPTRHATPGELV"
     gene            6769..7467
                     /locus_tag="VF517_05850"
     CDS             6769..7467
                     /locus_tag="VF517_05850"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX8052497.1"
                     /translation="MAGRHYVKFTFLRLDPAWRRLDQDERARHKREFAAGCNDFAEDH
                     FLRAYSLVGTRGDADLLVRCSAPRLDAIHDLHVVLNQSGLMGWAEVSHSFLAMTKESV
                     YSDEPQPLAPREGSERQFLMVYPMWKRRDWYSLPAEERMRVMRGHIETGRRYAGIEIN
                     TAYSFGLDDQEFVVSFNADDPGEFLDLVQELRGTESSAYTESETPIFTCIAETVERAL
                     DALDGQPVAAAQLT"
     gene            7464..8513
                     /locus_tag="VF517_05855"
     CDS             7464..8513
                     /locus_tag="VF517_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8052498.1"
                     /translation="MSTNGNQPDGEIRDITVIGAGPVGLITAFWAGMREASAQIIDSL
                     PDLGGQLTTLYPEKWIYDVPGYPRILAKDLVDQLREQAVDQFDVPVSLETTADRVEYE
                     DDGVMRLVTDKGDFLSRTIIIAGGHGAFEPKKLPGFDMTPWEGRGAHYLVKEKAAFAG
                     KKVVIVGGGDSACDWVLNLLDTADDITLVHRREGFRAHEVTVKEIEDAAAAGRVNVRT
                     PFQIKEIEGDGGIERVKIFNSEDEGHEEELDCDAVLLQLGFKTALGPLKEWDLEVEKG
                     AIKVDSLMRTSMDGVWAAGDITTFDGKLKLIATGFAEAAIAVAQAVHHIRPDTKIQPK
                     YSTNTGVPGSVEGQV"
     gene            complement(8530..9285)
                     /locus_tag="VF517_05860"
     CDS             complement(8530..9285)
                     /locus_tag="VF517_05860"
                     /inference="COORDINATES: protein motif:HMM:NF039447.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX8052499.1"
                     /translation="MEPLGLFFPDRFEWGWGGYAALVGVGALVAVMKTWPRDSISQSL
                     PPDNVRVEIRVGDVLKQRGNVIVGACDTFDTALEQQIIARNSVQGQLLTEVFEGDRDR
                     LDTEIAAFLPPNGQVDDAKTFGKKVRYPIGTVAMVKSGDTRFFLPAFTRMSSDRPPRV
                     ESSIPELHLALAETWAAIDAAGQREPIHTPIIGSNLGRIKLTKTLLIQMIVLSFVGSS
                     RRGPELVVHVTPKDAEDVDMAMLEGWLRALCAA"
     gene            complement(9384..9893)
                     /locus_tag="VF517_05865"
     CDS             complement(9384..9893)
                     /locus_tag="VF517_05865"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8052500.1"
                     /translation="MAYRNKTFVSFASEDIHCYRMMQAWRQNANIAFDFHDAHDLNTA
                     LDTSRPETIRRRLRQRFANTKQVVLLVSRDTRLVARRPSRFLYYEVEVIRRLGLPVVF
                     AHLDGSRRVNRERLPSKLLQPYTICVSFQPKIIRFALDRYVYSYAKHRHVRSGPHFYG
                     KAIYEPLGL"
     gene            complement(10003..10815)
                     /locus_tag="VF517_05870"
     CDS             complement(10003..10815)
                     /locus_tag="VF517_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8052501.1"
                     /translation="MRLVTYDSGSGPRSGVLRDGGIVDLAAGGLGALLRAGGAAEDPG
                     GAPTVSLEEARLLPPVPDPDKIVCIGLNYRTHAEEAGLEPPAVPTFFPKYRNALAAPG
                     TDVALPPYSDKVDYEAEVAFVIGRPCKDVPEEEALDAIAGFTLLNDLSARDYQFMTQQ
                     WAPGKVFDGSAPCGPALVTPDEAGAHDGIEISLTLNGEEMQRDTTASLIHSIPALVAF
                     LSKLMTLEPGDLVSTGTPAGVGSTRDPHVWLKPGDECVVESPTLGRLVTRLA"
     gene            10860..12383
                     /locus_tag="VF517_05875"
     CDS             10860..12383
                     /locus_tag="VF517_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX8052502.1"
                     /translation="MERLAVVDLGSNSFRLVVYGYEEGRWWQHADEIREAVRISEGIG
                     DDGRLKRKPMERALRTAEVFDAYCRSSGIDQVEAVATSAIRDAPNRDELLGALRERTA
                     LPVRVISTAEEARYGYLAIANSTTLGDGFGVDIGGGSVQVMHLRERLLDASDSWPLGA
                     VRMSERFLPGDEAGEKAVKALRKHVRKRVGEAEWYGRVNGDRLVGIGGTIRNLASAVQ
                     RRAGVDYPDAQGFVVERGALEELIEELASMPASKRARVPGIKPDRGDVILGGAIVLTA
                     LMEAGRFDELDVTEAGLREGVFFEHFLAGADPPLFPDVRRAAVENLALRYRGHDIAHE
                     RHVADLSLQIFDELGRAGLHELGPNDRELLWSACLLHDIGTAVDYDDHHKHSLYLIRN
                     AGLPGFKPREVALIALIARYHRKGTPDAAPLGALAKKGDAHRLDVLAGIVRLAEQLER
                     SRDRSVTGVRIASNGDGAVTLEPETAADASVAIWSARRNADLLGDAIDRRVEVAPGR"
     gene            12503..13501
                     /locus_tag="VF517_05880"
     CDS             12503..13501
                     /locus_tag="VF517_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8052503.1"
                     /translation="MKRVLSAAVLAAIMVAGATASAQDEPKPPKTLPPGSTIAGVAVG
                     DLGPKRARAAVEDALARTYGRRLFVKGRGRRLILGTSRLGQTIHYDRMVKAAFAQAAT
                     SNGLPVNVKLDRSLDRGKLRAAIGLIGARIDKPARNASVRLGVLRPSIRRPHNGLAVN
                     RVKLRRALVAEILRPNQTRLVTVSLVRTRPAIGVNRLRSIYGTYISIDRSTFRLRLFK
                     RLRRVRTYGIAVGAAGHGTPGGLFRVRGKDYNPAWHVPNSAWAGSLAGQTIPAGDPRN
                     PLRVVFMSLGGSIGIHGTSEPWSIGSRASHGCIRMRAGDVRNLAGRTPVGTPVLIR"
     gene            13549..>13998
                     /locus_tag="VF517_05885"
     CDS             13549..>13998
                     /locus_tag="VF517_05885"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX8052504.1"
                     /translation="MAKEPIPTGRIRRTAKVGGLVGGQAARAAFTRAANLTRSDEKRS
                     EALDRRYLAAAQQMVEVLGSMRGAAMKLGQVISFLDVSAIPPEYRDVVQDALAQLRNA
                     APEVPFADMRRVLESDLEQPIGEAFAEFDEHAVAAASIGQVYRARLHD"
BASE COUNT         2178 a         5006 c         4963 g         1851 t
ORIGIN      
        1 ctgtctcccg cgatggaggt cgttgtcgag gcggtgagga tgctcccggc gacgttccta
       61 ctgaagccgg tcgacttcgg cggcgatgcg tcctcgacgc ggtggcggag gctcggctgg
      121 tgagaggcgc gatgcacgag gccaccgtcc tctacgaccg cgactgcggc ttctgccgct
      181 ggaccctcgc gcagatcctc gcctgggacc gggccaagcg cctgcggccg gtgccgatcc
      241 agagcgagga gggccagcgg ctgctcgcgc gggtgccgga ggccgagcgg ctcgagtcgt
      301 ggcacctcgt gacgcccgac ggcgcggtga agtcggccgc ggagggattt ccgggtctgt
      361 tctcgcggct cccggcgggc gcgccgatcg cccggcttac cgcgctcctc cagggcccgt
      421 cgtcggtggg ctaccggctg ctcgtggcca accgaacccg gctggggcgg cggatcagcc
      481 aggaacgtcg tgaccgcgcg accgaggcgc tcgacgcgca cgagcgcgcc gcgcgcgcgg
      541 cagccgcggc ctaacgctgg cgcgccaccg ggccgataga cccgcgtggg accggagcta
      601 ccggaggggg agccacagcc gcagcagccg gccgcgcccg tgctcgacga ggcgtccgtg
      661 cagcgacgcg tcttcgtggt ccgcatggcc gcgtggggca ccgtgctcct gctcgcggtc
      721 atcgccttca acctgcgcgc cggcgcctcg gccgacaccg accagggcgc caacgggccg
      781 caggtcaacg gccgcacgag ccagggtcag gcgatctggt cgatcgcgga ccgtgagcag
      841 gtccgcgaga tccagatgtc gtggcagttc aagtgcgagg gcggcggccg gctcgagccg
      901 ttcgggggca cgttccggga cagcaccgac cacttcgact acgacggagc gaagttcaag
      961 gtcgcgatcg aggacgagat gccccagggc aaggacggct ggaccgccca cctcaaggcc
     1021 gaggtcggcg gcgaggtgca cgcggacggc caggcggttg gtaccagcgc ggcggtgatg
     1081 tggttcacgc ggacgggcca gcggggtgcg gtctgccgct cgggcccggt gcgctggtcg
     1141 acgccctagc cgccttcgtc gagccgccgc cggaacgccc ccgagccaac caccttcgcg
     1201 cccgccgcgc gcacggcctc ttcgagctcg tggtccgagg tcacaaccgt cagtcccccg
     1261 gggtccgggt ccgtacgcac ccaatcggcg atcgcgtggt ccgccgcgtt acgcccgccc
     1321 ggcgcgaacc cgacctcgac cagcccgcca ccgtcgagcc caaacgggtg cccgtcgaag
     1381 acaaccgcca cggtgtcccc ggtagccgcg gcaaacgttt cgagctgggt gatcagccgc
     1441 cgcatggccg cacgccgatc ccgccaccac ccatcgggcc gcgacccaac aacattcatc
     1501 ccgtcgacga gccaccgggg gcgagagtcc atttgtgggt cctatagcac tatgaaatgg
     1561 actctcacct ggccagattg gcggcgaccc aggccgaggt ggtcgcggcg tggcagttgc
     1621 ggcaggcggg ttggagcgct acgaagatca ggcatcacgc cgcccgcggg gcctggcggc
     1681 gcgttcatcc cggcgtctac gtcctgaccc gggcccagcc gagccaggtg cagctgtggt
     1741 gggcggcagc cctgacgacg cccaccagct tcctcagcca cggcagcgcg gccgcgtgct
     1801 acgggttcca ccgcttcagc aagccgttcg aggtggtcac ccggccgggg cagggtgggc
     1861 ggcggcggct cggtcgcctg ctggtcttcc ggtcgaagcg ccttcagggc gagaccacgc
     1921 gacacacggg gatcccgatc acgacggccg aacggacact ggtcgacgtc gccgcggggc
     1981 tggacgcgaa acgcgtcggg cgctgcttcc gtgaggcgat acggctgaag accacgaccg
     2041 cggcgcgggt cgcccggtgc gtcgaaagac acggcgatgc tcccacgcta ctggccgcgg
     2101 tcgcccggcg ctacgcgaag ctgccctacc ggcgcacgcg ctccgatgcc gaggcgcggg
     2161 cgctcgagct gctggacgat gccggcattc ggccgccggg ggtgaacgtg aggatcggcg
     2221 gtgaggaggc ggatctggtc tgggtagagc ggcggctgat cgtggagatc gacgggccgc
     2281 agttccacct gttccgcgac gaggacgccc gcaaggaggc gatatggcgc gacgcggggt
     2341 tcaccgtgcg ccgcgtgccg agctcgaccg tgtacgacga cccggcggcg ttcgtcgcgt
     2401 gctgcgtcag gccctagacc gggcggcctc ggcctcgcgg cgctgccgcg tggccgagag
     2461 ctcgacgggc gccgcgtgca gtgcccgcag gcgcaggtcg cgccacgaca tgccctcgcg
     2521 caccagagcg ggcatgccca gcgtgaacgc ggtggcgatc tccaccgcct ggaggatgat
     2581 cccgtaggcg aatgcgtcgg cgtggccgac cccgtaagcc gatagcaccg ccacgcaggc
     2641 cgcctggaag acgccgatgt tcgacggcgt ggccgggatc gccgcggtca cgttcaccgc
     2701 gaacagcacg gccgccgcgg cgcccatgcc cgcccggtcg tcgaggccca aggcgatcag
     2761 gagcgtgtag caggcgaccc actggatggc ccaggcgccg agctgcgtgg ccgcggccca
     2821 ggcgccgagc ttgggccggc ggaagacgcg caggccacgg cgggcatcga ccatcgcgcg
     2881 gcggaccgtc gaggcgagct gctgcacgcg cgcgaagcgc gacggcttgc ccttgcgcag
     2941 caggaacggc gcgagcgcga ccaggatcag cgcgacgacc ggcgcgatcg ccacccacag
     3001 cagcgagcgc tcgcggccct cgaacagccc gaccgtcatg aacatgatcg agccgagcac
     3061 cgcgagggcg aagaggttga ggatcgtctg cgagaccagc gtgccgagca cggtggggag
     3121 ccgctcgcgc acgcgcccga gacgccgcgc cacgatcagc gcgcgcgacg gctcgccgag
     3181 gcgcgcgggc agcgtggccg acatcagcac gccgatcatc acgccgcgcg ccgcgtcgcg
     3241 gcggcgcacc ttgagcccgg gcagcgcggc ccgcaggatc gcgtgccagg actccgcgcg
     3301 cagcagcatc gagctgcaca tgagggcgaa cgccacgaag acccaccacg gcgtggcggc
     3361 gatcatggac tggccgatcg attcccagcc gatccgctgg aaggccaacc cggcgaggcc
     3421 gatggccgcg ccgccggcgg ccgcgatgcc cactcgccgc gccacgcgca ccgcccggcg
     3481 ccggcgtgcg cccggcagct cgggttcgac cgaggggatc cggcgcggcg ggttgcgcgg
     3541 cagcccgtcg gcgggcgcga agcccgtgcg cagcgcgacc cgcgcggcgc gaccctccgg
     3601 caccaccgcg gcgcgctcga tcgcgcgctc gtagctctcc atcacctggt cggtcacgtg
     3661 cggccatgag aagcgctggg cgccggcgcg cgccgcggcc cccatgcgcg cgcggcgctc
     3721 cgggtcggtg gcgagcgcca gaagagcctc gccgagctcg gcggggtgcc cggcgggaac
     3781 gagcacgccg tcgacgccat cacgcaccac gtcgcggtac ccggcgatgt cggaggccac
     3841 cacgggcgtg cccgcggcga acgcctccgt gagcaccatc ccgaagctct cgccgccgag
     3901 cgacggggcg caaaccacgt cggcctcgtg cagcagccgc cacttctcct cctcggtcac
     3961 gcggccggcc acgtgcacac cctcggggtc gagcaggtac ggctcgacct cctccggcgt
     4021 cgcgcccgcc accgtgagcc gcgttcgcac gccggcctcg cgcagcgcct cgaacgcacg
     4081 cagcagcacc ggcagcccct tccgctcctc ggcgcggccg acgaacagca gccgcagctc
     4141 gcgcccctcg ggcttcggcc cggtcggcgc cgccgacagg tccacgccgt tcgggatcac
     4201 ccggtagttc ccgccgtaga agcgctgcgc cgtccagcgc gccgcctcgg agacggcgat
     4261 ccgctcgtgc agcttcgagt agacgcgccg ggcgttgagg acgttggccg tgaagaggtg
     4321 gaccggcgtg ttggtcgagt acgtgtggaa cgtgccgacg agcggcgcgc gggtgttctc
     4381 ggtcgcgacc cagctcagca ccggcgtgtt gggctcgtgg acgtgcacca cgtcgaagtc
     4441 gccatgacgc agagtgcggt tcagcgtggc caccgcgtcg gccgagatcg agatgttcga
     4501 ctgcgagccg ttcatcgaga acccgtacgt gcgcccgagc gagacgaagt ggtccggcag
     4561 ctcgcgcgtg gcgggacggc cgccacgatg ggtcaggcga gcgacccggt catccgggtc
     4621 gaacggggcg agcaggcgta cgtcgtggcc gcgggcaagc aactcgtccg ccagagcctc
     4681 ggcgtgccga ccgaccccgc ccggataact gaaggagtag ggcgagacca gcgctatgcg
     4741 cactgtcgca tcctagacca gctcgcccgg agttgcatgg cgcgttggcg ctccctgcgc
     4801 atctcggcgc gctgccctgc gcggcgccgc gccaggggcc ccgcgcccgt agccgagcgc
     4861 cagcagcagc ccgaagaagc ccgcgacgaa gatcacaggg cccgagtcgt tggagagcgc
     4921 gccgatcacc gtggccgcca gcgcgcccca gagcccggcc accaaggcgg ggtgcgggcg
     4981 gagcgggcgg aggaggtctt ggcggcggcg cactccccag atcatgcaca cgatcccggt
     5041 cgccaccagc acgatcatct tgaccgtgtc gaggccgttg gccgagatca ggagccgccg
     5101 cttcacgatc tcggcaaggc cgctggcgcc gtccccctcg agcaccgtgc gcgtgaggtg
     5161 cgccccaccg ccggtgagca ggtcgatccc gatcagcccc accaccgcca cggccggagc
     5221 catcaccgcc agggcgacgg cgcgccggct cggcccgccc ggcagcgacg ccaggaccgc
     5281 cccggcggcg cccgcgccga gcgtgatcac cccgcccacg tcggccccga gccgcccggc
     5341 gccgatcgtc acggccgcga ccaggcagcc gagcgcgaag ccgatcggca cgcggcggcc
     5401 ggcgaagagc gtgagcagcg cggcgaggcc gaacagcacc tccaggctga gaaggatctc
     5461 gagctcgttc ccgatgccga agaagcgcgc gccgcccttc gggttcgggc ccgcgagcga
     5521 ggcgccgatg agaggcgagc cgcgcgcgag gtcgatcgcg tgcgcggcga acaccgcgag
     5581 gcccggcagc gcgggcgcca gcggccagcg caccaggcgg tccgtggcgc gtccgagcgc
     5641 gagcgacccg cccgcgagca ggatcgtctc ggccaggcgc gtgggctgca ggtaggcggt
     5701 aaggagcgcc acgcccggca gccacatggc gcccaggaac ccggtgcgca gcgcctcgtc
     5761 gagcccctcg cggcggcgcc ggaaccacag cgccgtggcc agccccgcga acccgatcac
     5821 gaaccagcgc agcccgtggc cacggcgcga gaggaccacg tccaggcgcg ccatccgcag
     5881 ccggacctcc tccgggtcgc cgtccgggcg cgggtcgatg gtgcggccgt ccatcgcgtc
     5941 cggcaccccg atcccaaggt ggtcgagcac ggtggtcgcg tagtcggtca cggccacgag
     6001 cccgagccgg cgcgtggtcg gcgagaagat cacctgctgc tcggcgcccg gcccgagcat
     6061 cccggcgggg agcagccgcc cgcggccggc cggtggagcg cgcaccacca ggatcaggtc
     6121 gtccggcccg cgcgcggcca ccacgcggtc gagcgcggcc aggcccgcct cgtcgctcgg
     6181 gaagcgcgcg accaccacct cgtggtcgct ccacagctcc agggtgcgat cggcgaacgt
     6241 cccgatcgtc cccagcgaca cgcgctcgac gcggccctgg cgatcggccg ccacgaccgc
     6301 ctcggtctgc ggaaatccga ccacgcccac gtacgcggcg gacgccccgc ggcggcgcag
     6361 ggtgtccccg agcagccccg ggaccacgtc gccgggcgcg tcgtcggctc gcgcgcgtgc
     6421 gcggcgaaat cccttcacct gcccgccgcc atcgggatcg aggcgcaggt cgagccgctt
     6481 cagcttgtcc ggatacgcgc tgttcgcgat ccgcgatccc tggctcatgt cgagcaccat
     6541 ctggcgcttg gagtaggccc cgatcgacgg gctggccatc ccgtaggaga agtcgcgctg
     6601 ggccaggtcg aacagcagcg gcgacttcgg cgcgggctcc gtgggcacga acgcgatcag
     6661 cgcgcgcggg gccgccgcgg ccgccgcggg cagcgcgcag aggagcccga acgcgcccaa
     6721 aatcgccaga aaagcggctc tgcgacgcat ttctacactc tatgaagcgt ggccggccgc
     6781 cattacgtca agttcacttt cctgaggctc gaccctgcct ggcgcaggct cgaccaggac
     6841 gagcgcgcgc gccacaagcg cgagttcgcc gccggctgca acgacttcgc cgaggaccac
     6901 ttcctgcgcg cctactcgct ggtcggcacg cgcggcgacg cggacctgct ggtccgctgc
     6961 tcagccccgc ggctcgacgc gatccacgac ctccacgtcg tgctcaacca gagcggcctg
     7021 atgggctggg ccgaggtcag ccactcgttc ctcgccatga cgaaggagtc ggtctactcc
     7081 gacgagccgc agccgctggc cccgcgcgag ggcagcgagc gccagttcct catggtctac
     7141 ccgatgtgga agcgccgcga ctggtacagc ctgcccgcgg aggagcgcat gcgcgtcatg
     7201 cgcggccaca tcgagaccgg ccgccgctac gcgggcatcg agatcaatac cgcgtacagt
     7261 ttcggcctcg acgaccagga gttcgtcgtg tcgttcaacg cggacgaccc gggcgagttc
     7321 ctggatctgg tgcaggagct gcgggggacg gagagcagcg cctacaccga gtccgagacc
     7381 ccgatcttca cctgcatcgc cgagacggtg gaacgggcgc tcgatgcgct cgacggccaa
     7441 ccggtggcgg cggcacaact gacatgagca cgaacggaaa ccagccagac ggagagattc
     7501 gcgacatcac ggtcatcggc gccgggccgg tggggctgat caccgccttc tgggcgggca
     7561 tgcgcgaggc gagcgcgcag atcatcgact cgctgcccga cctcggcggc cagctcacca
     7621 cgctctatcc cgagaagtgg atctacgacg tgccgggcta cccgcggatc ctcgcgaagg
     7681 acctggtcga ccagctccgc gagcaggcgg tcgatcagtt cgacgtcccc gtgagcctcg
     7741 agaccaccgc cgaccgcgtc gagtacgagg acgacggcgt catgcgcctc gtcaccgaca
     7801 agggcgactt cctcagccgc acgatcatca tcgccggcgg ccacggcgcc ttcgagccga
     7861 agaagctgcc cgggttcgac atgacgccgt gggagggcag gggcgcgcac tatctcgtca
     7921 aggagaaggc ggccttcgcc ggcaagaagg tcgtcatcgt cggcggcggc gactcggcct
     7981 gcgactgggt gctcaacctg ctcgacaccg ccgacgacat cacgctcgtc caccggcgcg
     8041 agggcttccg cgcgcacgag gtgacggtca aggagatcga ggacgcggcc gccgccgggc
     8101 gcgtgaacgt gcgcacgccg ttccagatca aggagatcga gggcgacggc ggcatcgagc
     8161 gggtcaagat cttcaactcg gaggacgagg gccacgagga ggagctcgac tgcgacgccg
     8221 tgctgctcca gctcggcttc aagaccgcgc tcggcccgct gaaggagtgg gacctcgagg
     8281 tcgagaaggg cgcgatcaag gtcgactcgc tgatgaggac gtcgatggac ggcgtctggg
     8341 ccgcggggga catcacgacc ttcgacggca agctgaagct gatcgccacg ggcttcgccg
     8401 aggcggccat cgcggtggcc caggccgtgc atcacatccg gccggacacg aagatccagc
     8461 cgaagtactc gaccaacacc ggcgtgccgg ggtcggtcga gggccaggtc tagccgcccg
     8521 ccgcgtccac taggcggcac ataaggcacg caaccagcct tcgagcatcg ccatgtcaac
     8581 gtcttccgca tcttttggcg tgacatggac cacgagctcc gggccacgcc gcgaactacc
     8641 aacgaaggac agcacgatca tctggatcaa caaggtcttg gttaatttaa ttcgacccag
     8701 gttcgatcca atgattgggg tatggattgg ttctcgctgc ccggcggcgt cgattgccgc
     8761 ccatgtttcg gcaagcgcca aatgtagttc gggaatactg gattcgaccc gaggtggccg
     8821 atcggaggac attctcgtaa acgctggcaa gaagaaccgc gtatccccac ttttcaccat
     8881 tgcgacggtg cctatcgggt aacgaacctt ttttccgaac gtcttagcgt cgtcgacttg
     8941 cccattgggc ggcaagaacg cggcgatctc cgtgtcgagt cgatctcggt ctccctcgaa
     9001 cacctcagta agtagctgtc cctggacgct gtttcgagca attatttgtt gctccagggc
     9061 agtatcgaaa gtgtcacacg cgccgacgat gacgttgcct ctctgcttga ggacgtcgcc
     9121 gacacgtatc tcaacccgca cattgtcggg cggcagcgac tgcgaaatcg agtcgcgtgg
     9181 ccatgtcttc attactgcca cgagcgcgcc gactccaaca agcgccgcat agccccccca
     9241 accccactcg aaccgatcag ggaagaacag cccgagtggc tccaagatca gccagagtcc
     9301 cccgaacgcg gtgacaaagc taagcgcgaa ttgttttctc ccagaccgac gagcgtaatg
     9361 tgcctttgtc ctggcgttca gtttcacagt cctagaggct cgtagatcgc cttgccgtag
     9421 aagtgcggtc cggagcggac gtggcgatgc ttggcatatg aatagacgta tcggtctagg
     9481 gcgaatcgaa tgatctttgg ctgaaaggaa acacagatcg tgtacggctg gagcaatttg
     9541 ctcggaagcc gctctcgatt tacgcggcgg gaaccgtcga ggtgagcgaa aacgacgggt
     9601 agaccaaggc gcctaattac ctcgacctcg tagtagagga agcgggacgg cctccgcgcg
     9661 acgaggcgcg tgtcgcgact cacgagcagc acaacctgct tcgtgttcgc aaaccgctga
     9721 cgcaagcggc ggcgaattgt ctcgggccga ctggtgtcga gagccgtatt caggtcatgg
     9781 gcgtcgtgaa agtcgaacgc gatgttcgcg ttctggcgcc acgcctgcat catccggtag
     9841 cagtggatgt cttcgcttgc gaagctaaca aatgtcttgt tgcggtatgc catgacttcc
     9901 gccgcagtgg cacaacgacg ctcggcagag taccggtgcg ggaacacggc tggcgacgcg
     9961 gttgcttcgg cgtgcccggg cggtcgaggg ccaggtctag gtctaagcca ggcgcgtcac
    10021 gagccgaccc agcgtcggcg actcgaccac gcactcgtcg ccgggcttca gccagacgtg
    10081 cgggtcgcgc gtgctgccca cgcccgcggg cgtgccggtc gagacgaggt cgcccggctc
    10141 gagcgtcatc agcttcgaga ggaacgccac cagcgccggg atcgagtgga tcaggctggc
    10201 ggtcgtgtcg cgctgcatct cctcgccgtt cagcgtgagc gagatctcga tcccgtcgtg
    10261 cgcgccggcc tcgtcgggcg tgacgagcgc ggggccgcac ggggccgagc cgtcgaagac
    10321 cttgccgggc gcccactgct gggtcatgaa ctggtagtcg cgcgccgaga ggtcgttcag
    10381 cagcgtgaag ccggcgatcg cgtcgagcgc ctcctcctcg ggcacgtcct tgcaggggcg
    10441 gccgatcacg aacgccacct cggcctcgta gtcgaccttg tcgctgtacg ggggcagcgc
    10501 cacgtcggtg ccgggcgccg ccagggcatt gcggtacttc gggaagaagg tgggcaccgc
    10561 gggcggctcg agcccggcct cctcggcgtg ggtccggtag ttcagcccga tgcagacgat
    10621 cttgtccggg tccgggaccg gcgggagcag cctcgcctcc tcgagcgaga cggtgggcgc
    10681 gccgcccggg tcctcggccg cgccgccggc acgcagcagc gcgccgaggc cgccggccgc
    10741 gaggtccacg atgccgccgt cgcgcagcac gccggagcgc gggccggagc cggagtcgta
    10801 ggtcacgagt cgcatgccgc gcgcattatg ccgccgacgg cccggccatc atcccctggg
    10861 tggaacgcct cgccgtcgtg gacctcggat cgaactcgtt ccggctcgtc gtctacgggt
    10921 acgaggaggg ccgctggtgg cagcacgcgg acgagattcg cgaggccgtt cggatcagcg
    10981 agggcatcgg cgacgacggc cgcctgaagc gcaagccgat ggagcgcgcg ctgcgcaccg
    11041 ccgaggtgtt cgacgcctac tgccgctcct ccgggatcga ccaggtggag gccgtcgcca
    11101 cgagcgcgat ccgcgacgcg cccaaccgcg acgagctgct gggcgcgctg cgcgagcgca
    11161 ccgcgctccc cgtccgggtg atctcgaccg ccgaggaggc ccgctacggc tacctggcga
    11221 tcgccaactc gaccacgctc ggcgacggct tcggcgtcga catcggcggc ggcagcgtgc
    11281 aggtgatgca cctccgcgag cgcctgctcg acgcaagcga ctcgtggcct ctcggcgccg
    11341 tgcggatgag cgagcgcttc ctccccgggg acgaggccgg cgagaaggcg gtgaaggcgc
    11401 tgcgcaagca cgtccgcaag cgggtcggcg aggccgagtg gtacgggcgg gtgaacggcg
    11461 atcggctggt ggggatcggc ggcacgatcc gcaacctcgc gtccgcggtg cagcggcggg
    11521 cgggtgtgga ctacccggac gcgcagggct tcgtggtcga gcgcggcgcg ctcgaggagc
    11581 tgatcgagga gctggcgtcg atgccggcgt ccaagcgcgc ccgcgtgccc ggcatcaagc
    11641 ccgaccgcgg cgacgtgatc ctcggtggcg cgatcgttct caccgcgctg atggaagccg
    11701 ggcgcttcga cgagctcgac gtgaccgagg caggcctgcg cgagggcgtc ttcttcgagc
    11761 acttcctcgc cggcgccgac ccgccgctgt ttcccgacgt gcggcgagcc gcggtcgaga
    11821 acctcgccct gcgctaccgc ggccatgaca tcgcgcacga gcgccacgtc gccgacctct
    11881 ctctccagat cttcgacgag ctcggccgcg ccggcctgca cgagctcggg ccgaacgacc
    11941 gcgagctgct ctggagcgcg tgcctgctgc acgacatcgg gacggccgtc gactacgacg
    12001 accaccacaa gcactcgctc tacctgatcc ggaacgcggg gctgccgggc ttcaagccgc
    12061 gcgaggtggc gctgatcgcg ctgatagcgc gctaccaccg caagggcacc ccggacgccg
    12121 ccccgctcgg cgcgctggcg aagaagggcg acgcgcaccg gctcgacgtg ctcgccggaa
    12181 tcgtgcggct ggccgagcag ctcgagcgct cgcgcgaccg ctcggtcacc ggagtgcgga
    12241 tcgcgagcaa cggcgacggc gccgtgaccc tcgagccgga gaccgccgcc gacgccagcg
    12301 tggcgatctg gtccgcccgc cgcaacgcgg acctcctggg cgacgcgatc gaccggcggg
    12361 tcgaggtcgc gccgggccgc tgacgaccaa ctggtcgatc taatgcacgc tatgcgggcc
    12421 taagatcgac cagtagtgcc aaaagccgtg catccggcgg ttccccctgg aaatcgtggc
    12481 gttcggcagg gatcatgcgc gtgtgaagcg tgtcctaagt gctgccgttc tggcggcgat
    12541 catggttgcc ggggcgaccg cgagcgccca ggacgagccg aagccgccga agacccttcc
    12601 ccccggctcc accatcgccg gcgtcgccgt cggcgacctc ggccccaagc gcgcccgggc
    12661 cgccgtcgag gacgcgctgg ccaggaccta cggccgccgc ctcttcgtca agggccgcgg
    12721 caggcggctg atcctcggca cgagccgcct gggccagacg atccactatg accgcatggt
    12781 caaggccgcc ttcgcacagg ccgcgacgtc gaacggcctg cccgtgaacg tgaagctcga
    12841 ccgctcgctc gaccgcggca agctccgcgc cgcgatcggt ctgatcggcg cgcggatcga
    12901 caagccagcc cgcaacgcca gcgtccggct cggcgtgctg cggccctcga tccgcaggcc
    12961 gcacaacggg ctcgccgtga accgcgtgaa gctgcgccgc gcgctggtcg ccgagatcct
    13021 gcgaccgaac cagacccgcc tcgtcaccgt gagcctggtg cgcacgcggc cggcgatcgg
    13081 ggtcaatcgg ctgcgctcga tctacggcac ctacatctcg atcgaccggt cgaccttccg
    13141 cctgcggctc ttcaagcggc tgcgtcgcgt gcgcacctac gggatcgccg tcggcgcggc
    13201 cggccacggg accccgggcg gcctcttccg ggtccgcggc aaggactaca accccgcctg
    13261 gcacgtgccc aactcggcct gggccgggag cctcgccggt cagacgatcc ccgcgggcga
    13321 tccgcgcaac ccgctgcgcg tggtgttcat gtcgctcggc ggcagcatcg ggatccacgg
    13381 cacgtcggag ccgtggtcga tcggcagccg cgcctcgcac ggctgcatcc gcatgcgcgc
    13441 gggcgacgtg cgcaacctcg cgggccgcac gccggtcggc acgcctgtcc tcatccgcta
    13501 gcggtcgaac gtcaccctcg ggcgcggcga gcgacgtacc ctcagtgcat ggctaaagag
    13561 ccgatcccga ccggccgcat ccgccgcacc gcaaaggtcg gcggactggt cgggggccag
    13621 gcggcgcggg ccgccttcac gcgggccgcg aacctgacgc gctccgacga gaagcgcagc
    13681 gaggcgctcg accgccgcta cctggccgcg gcgcagcaga tggtcgaggt gctcgggagc
    13741 atgcgcggcg cggcgatgaa gctcggccag gtgatctcgt tcctcgacgt gagcgcgatc
    13801 ccgccggagt accgcgacgt ggtccaggac gcgctggcgc agctccgcaa cgccgcgccg
    13861 gaggtgccgt tcgccgacat gcgccgcgtg ctcgagagcg acctcgagca gccgatcggc
    13921 gaggcgttcg ccgagttcga cgagcacgcc gtggcggccg cctcgatcgg gcaggtctac
    13981 cgggcgcggc tgcacgac
//
LOCUS       DASVPL010000117         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_209609, whole genome shotgun sequence.
ACCESSION   DASVPL010000117 DASVPL010000000
VERSION     DASVPL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209609"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VF517_05890"
     CDS             <1..900
                     /locus_tag="VF517_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8052505.1"
                     /translation="TDVENYPGFPEGIMGPEMMQRFRDQAERFGTRFLSDEATRVELA
                     TDGGLHRVWVGDDEYQARSVILAMGAEHKKLGVPGEEELSSRGISYCATCDAAFFRDR
                     KIMVVGGGDSAMEEAIFLSKFGSRVQVVHRRDDFRASQIMIDRAREIENIELVTPYVV
                     DRFEEGEGGTLARAVLKHSDTGEERVEEVDGGFIAIGHEPSSQLVRDQVETDENGYVV
                     TDGKTTRTGIPGVLAAGDLVDHTYRQAITAAGSGCQAALDAEWYLRDQPPDPGAHWMK
                     PDEVEAAAEQMAADVAADPASAR"
     gene            complement(971..1432)
                     /locus_tag="VF517_05895"
     CDS             complement(971..1432)
                     /locus_tag="VF517_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052506.1"
                     /translation="MLAATNTGTPPMLLPLPQLSQSAGGPGAYPRSAPAEGGRTARVE
                     RLQRSDSPSLPFSGFSVLALVLAGIASITAGRRLSEAVAVADGATDEPGAAARRAAEP
                     LVAEPLRRQADPLRLQAERQAAGPARSHNRAPLYVGAALFAVAAVTAARSR"
BASE COUNT          218 a          633 c          696 g          214 t
ORIGIN      
        1 accgacgtgg agaactatcc gggcttcccg gagggcatca tggggccgga gatgatgcag
       61 cgcttccgcg accaggcgga gcgcttcggc acccgcttcc tctccgacga ggccacgcgg
      121 gtcgagctcg cgaccgatgg cggcctgcac cgcgtgtggg tcggcgacga cgagtaccag
      181 gcgcgctcgg tgatcctcgc gatgggggcg gagcacaaga agctcggcgt gcccggcgag
      241 gaggagctct cctcgcgcgg gatctcctac tgcgccacct gcgacgccgc gttcttccgc
      301 gaccgcaaga tcatggtcgt cggcggcggc gactcggcga tggaggaggc gatcttcctg
      361 tcgaagttcg gctcgcgcgt gcaggtcgtg caccgccgcg acgacttccg cgcctcgcag
      421 atcatgatcg accgcgcccg cgagatcgag aacatcgagc tggtcacgcc gtacgtcgtg
      481 gaccggttcg aggagggcga gggcgggacg ctcgcccgcg ccgtgctgaa gcactccgac
      541 acgggcgagg agcgggtcga ggaggtcgac ggcgggttca tcgcgatcgg ccacgagccg
      601 tcgtcccagc tcgtgcgcga ccaggtcgag accgacgaga acggctacgt ggtgaccgac
      661 ggcaagacca cgcgcacggg gatcccgggc gtgctcgccg cgggcgatct ggtcgaccac
      721 acctaccggc aggcgatcac cgccgccggc agcggctgcc aggccgcgct cgacgcggag
      781 tggtacctac gcgaccagcc gccggacccc ggcgcgcact ggatgaagcc ggacgaggtc
      841 gaggccgcgg ccgagcagat ggcggccgac gtggcggccg accccgcctc cgcgcgctag
      901 cggcgccggc tcctcatcga ttggagcctt gctccgccat gcggtacaga aggctccaat
      961 cgacgcgcga ctagcgcgag cgggcggccg tcacggcggc cacggcgaag agcgcggcgc
     1021 cgacgtagag gggcgcgcgg ttgtgcgagc gtgcgggacc ggcggcctgg cgctccgcct
     1081 ggagccggag cgggtccgcc tggcgccgga gcggctccgc gacgagcggc tccgccgccc
     1141 gccgtgccgc cgcgccgggc tcgtccgtgg cgccgtccgc caccgcgacg gcttcgctca
     1201 gccgccggcc ggccgtgatc gaggcgatgc ccgcgaggac gagcgccagc accgagaagc
     1261 cgctgaacgg gagcgacggg ctgtcgctcc gctggagccg ctccacccga gccgtgcgtc
     1321 cgccctccgc cggcgccgac cgcgggtagg cgcccggccc gcccgcgctc tgtgagagct
     1381 gcggcagcgg aagcagcatc ggcggcgtcc cggtgttggt cgcggcgagc accagcgcgt
     1441 tcgcgggggc gtccccggct cccgcgggcg cgggcgcggt gggcgaaggg gtcgcggggg
     1501 tcgcgcggtg ccgacggttc cgacgatcag gcgcggcctt gcggtccgag ccgcgggccg
     1561 cgggcgccga gggccgatcg cgtccggtcg cgcgcgcctt cgggcgtgcg ccgccctccg
     1621 cgtgcctcct cgcatgtgag ccgttccccg cggggcgctg cggcggggac gccggccggg
     1681 gagcgggctt cccgcggccg tgccgtgggg cgggctttcc gtggccgtgc cggggtgtgc
     1741 cgtgagcggc ggagcccttc g
//
LOCUS       DASVPL010000118        13100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_368511, whole genome shotgun sequence.
ACCESSION   DASVPL010000118 DASVPL010000000
VERSION     DASVPL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13100
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368511"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..891)
                     /gene="dapF"
                     /locus_tag="VF517_05900"
     CDS             complement(40..891)
                     /gene="dapF"
                     /locus_tag="VF517_05900"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX8052507.1"
                     /translation="MRFEKWQALGNDYLIVEASELPFELTPGRIRRLCAPHTGVGSDG
                     VLLLSEPDEDGFVARLRIFNPDGSEAELSGNGAREAILYLRQRGWVDRDEFAIQTAAG
                     PIRPQITGPTTCRVDMGRASLTSKDFPSGGEDGTGTLAALGGELSFQHVSIGNPQCAI
                     RVAARDELERLELPAIGPAIEHAPQFPQRTNVSFWTKLAPNRIRARIFERGVGETLSS
                     GTGACGAAVAHVVRGGDSPVTVVLDGGELTVDVTEDLHVDLSGWAEPVYRGELSAELI
                     ARLRDTV"
     gene            complement(903..1508)
                     /locus_tag="VF517_05905"
     CDS             complement(903..1508)
                     /locus_tag="VF517_05905"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8052508.1"
                     /translation="MIGPAPSVRRAERSDLDEVVRTLTRAFYDDPVMRWFFPDDGNRA
                     ALNERFFRLRIAGLLAQEEVYTTDDHSGAACWAQPGRWEMGPLEGLMFVMRVLPMTRL
                     RTPLLARGWSAVDAAHPKEPHWYLAILGTEPESQGRGIGSALMQPVLDDCDRNEVPAY
                     LESSKRSNLDFYARHGFRVTEELTLPEGPTIWPMWRDPRRV"
     gene            complement(1505..2404)
                     /gene="miaA"
                     /locus_tag="VF517_05910"
     CDS             complement(1505..2404)
                     /gene="miaA"
                     /locus_tag="VF517_05910"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX8052509.1"
                     /translation="MSEVVAIFGPTGVGKTAVAVELADLLRARGEDPVAISADALQVY
                     RGLETLTGVASAAERVRLDHRLVAFVDPRESFSAGEFMPLAHAEIDRALESGRRPIVL
                     GGTGLYLRAALTELSLPPAPPPELRARWQRAVDERGPAALHAELRERSPRAAAAVAPT
                     DRTRIVRSLELLDLGEAPPPTGDSELWTGETRRPTRLFGLTRDRDDLYDRIDRRVDAM
                     VARGAADEVRRAAAAGVAHTARKALGFEELLAGDVEGMKRRTRNYAKRQLTWMRKLAG
                     VTVLDLTGREPADVAREIAGTLA"
     gene            2403..2798
                     /locus_tag="VF517_05915"
     CDS             2403..2798
                     /locus_tag="VF517_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052510.1"
                     /translation="MGRTIAPPSEDRPAEAAPRASAILESRPVVALLGLAAFAQALCF
                     AVVAGVVGLAIVRGGSAAGDHLAIGIVWLVALTLVGTVLCALAQAALIWHANETDALA
                     GTNRRRWLIRLTIWGPLAMPSYWRRYMRR"
     gene            complement(2795..4147)
                     /locus_tag="VF517_05920"
     CDS             complement(2795..4147)
                     /locus_tag="VF517_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8052511.1"
                     /translation="MSRNSGIPPGPRYPGWIQALGFWSRPLPYFERCRARYGKRFTLR
                     LPTVPPFVVLSDPSDVKEVFMAPPDVLHPGSGVARVLEPVLGSYSLIVLDEDPHMEQR
                     KLLLPALHGEKIQRLAGVMREVAEQDLASWPRGEPVELHPRFQKLTLEIILRSLFGLG
                     PGPRLDALRDKLAAALAYGDSFVSLVPPQPGNRLSPLLERVGPYATFKRGQREADRMI
                     FELIEERRRDPADRDDILSLLLEARHEDGSPMSGQEIRDELMTLLVAGHETTASALAW
                     AFERLPQHPEVVERLREEIDAGDGDDYLTATIYETLRDRPVLPTASPRLAMKPIEVGG
                     WSYPAGVILIPNSYLIHHDPEIYDDPYAFRPERFLDEAPGTYTWFPFGGGRRRCVGSS
                     FAMVELKIIMSAMLKSLDIVPDGGGREVAARRNITVRPSRGTRVVLRDRVREPALPAA
                     "
     gene            complement(4192..5520)
                     /gene="miaB"
                     /locus_tag="VF517_05925"
     CDS             complement(4192..5520)
                     /gene="miaB"
                     /locus_tag="VF517_05925"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX8052512.1"
                     /translation="MSRTYHVTTFGCQMNEHDSERMKGMLESLGYSEVPERDGADLIL
                     FNTCSIRESADNRLIGHLGEAKRLKTENPDRIVGVGGCWAQSIKDEVFERFPFVDVAF
                     GPGQVPKLAEFLTSDSLSAQGYFEFEGFTGHLPPKRARDFQGWVQISVGCNCVCSYCI
                     VPSTRGREVSRPAAELVAEVERLAADGVSEVTLLGQNVNSYGRDLPRDAKATFAELLA
                     MVDAVDGIERIRYTSPHPKDMREDVIRAHAELPALCEHIHLPLQSGSSRILKAMRRTY
                     DRGRYMDRVALIREHVPDCALTTDIIVGFPGETEADFEETLAVAEEVGYDSAFTFVFS
                     PRRGTAAADLDDQVPYPVKRERMQRLVEVVQRRATERAQRFVGRTMEVLVEGPSRTDP
                     DRLRGRTRHNKTVHFAGAAEPGSFTDVEIDSATSTTLAGRERVGSITQTV"
     gene            complement(5526..5945)
                     /locus_tag="VF517_05930"
     CDS             complement(5526..5945)
                     /locus_tag="VF517_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8052513.1"
                     /translation="MDQRLSLITLGTRDLPRARAFYEALGWHTNAGPDDDVVFFQAVG
                     MVVALWGRDQLAEDSGVTDSGGWGGITLAYNTRSPEEVDSVIEEARAAGGEIRREPGE
                     TFWGGYSGMFVDPDGHPWEVAHNPRWSIEPDGSTRLG"
     gene            complement(5974..6852)
                     /locus_tag="VF517_05935"
     CDS             complement(5974..6852)
                     /locus_tag="VF517_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052514.1"
                     /translation="MGRKRSIGLLTVVAMAALAAPAGATDNGWDYNNEFAKRPDDPRY
                     QVPVSTCVYTTRAPAAANIPIYGFGGRLLPFQIGTTRAPECGPNRLRISTLQRLTIGG
                     RLTYMNRGGGDYSQAHAHVAGSELNPPPDLLPYSARMGNGRSAPGCNLHLTSKPVSMP
                     GDMFYKSLSEGVGSGSSWANYGDPGKDDHGDSYNYILWNMPRRDTGASEAVLPGGGAI
                     MANIGPNTHVYACDVSQQVLNSYSYQTNTINGYTRWAYVHLTNPDGENLWGWFLVSYK
                     YGTGNEAEVGLYNWGS"
     gene            complement(6950..7141)
                     /locus_tag="VF517_05940"
     CDS             complement(6950..7141)
                     /locus_tag="VF517_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052515.1"
                     /translation="MRLFRSEEETPTDGAAMPVGRLNALAKRWYGNRLDPDWRPRTTP
                     ESQATLDAVGLTGAFWRLS"
     gene            complement(7148..7546)
                     /gene="merB"
                     /locus_tag="VF517_05945"
     CDS             complement(7148..7546)
                     /gene="merB"
                     /locus_tag="VF517_05945"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HEX8052516.1"
                     /translation="MTEREHALRAEIMRTFAATGAPPEPPEPPGDRATLHSLAEQHVV
                     VLDDRDRIVMAHPFAAPHADAKVESGGRTWWGSCAWDGLGIVAALALERASVTSTGVT
                     LEVESGEVEPGPVFHVAVPARNWWDDIAHT"
     gene            complement(7547..9325)
                     /gene="rny"
                     /locus_tag="VF517_05950"
     CDS             complement(7547..9325)
                     /gene="rny"
                     /locus_tag="VF517_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX8052517.1"
                     /translation="MLAAVVSAGVSALVVLLLRPRMITAAGHAPGAGAAGHAPGAAAA
                     GAGAGPGAGPGAPAPDPRSAGAPAPAPDSGATAPAPQRREASVTQAAAGVEAASPEHP
                     PGRFTRETGDFNRETGEQPGATHPAPAPSADIDAIRRDLESDLRARHADIEAELRERR
                     AEIARIEERTLRKEESVDLRLADLERRETALEDRRRNLEHEAEKLKAAKRDQLRELER
                     IAGLSAGQARQILLRELEEELRHDSAALVRQVEQEARREADQRARSILSTVMQRVAGG
                     HAAETTVSVVHLDSDDLKGRVIGREGRNIRALETLTGIDFIVDDTPGAVLLSGFDGVR
                     REIARLTLERLLQDGRIHPARIEEAYETARTEMESHIVEVGEQAVLDADIGTVHPELA
                     KLLGRLHYRTSYGQNVLAHSLECARLARLLAAELGASPKCAARAALLHDIGKAVTHEI
                     EGPHALVGGELARRYGESEAVAHAMEAHHNEVELRTIEAVIVQIVDSLSGARPGARGE
                     SLEQYTKRLRNLEEIAGRHTGVEKVYAMQAGREIRVIVHPEEVDDDTAALLSHRIARD
                     VERELEYAGQIKVTVIRERRSVDFAK"
     gene            complement(9874..11016)
                     /gene="recA"
                     /locus_tag="VF517_05955"
     CDS             complement(9874..11016)
                     /gene="recA"
                     /locus_tag="VF517_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX8052518.1"
                     /translation="MDKDKEQEKAAKARDAALNGALGQIERQFGKGSIMKMGDEQFQV
                     QVAAIPTGALSLDLALGVGGIPRGRIVEVFGPESSGKTTLLYHLLAEAQKTGGICAFI
                     DAEHAMDPVYAKRIGVDVDELLVSQPDHGEQALEIADLLIRSGAVDVVAIDSVAALTP
                     KAELEGAMGDQTVGLQARMMSQAMRKLAGNLNRANTLCIFTNQIREKVGVMFGSPETQ
                     PGGRALKFYSSQRLDIRRIETLKDGTEAVGNRVRVKVVKNKVAAPFRQAEFDIEYGKG
                     ISTEGCLIDLGLEHNLIQKSGSFFSYGDERIGQGRNNAKGFLRENPAMAKEIEAKIYA
                     ALGIEPARPLAAVPPPDEIVGDGNGQATEPVPEPTTGGVAPAEQAA"
     gene            complement(11393..11872)
                     /locus_tag="VF517_05960"
     CDS             complement(11393..11872)
                     /locus_tag="VF517_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052519.1"
                     /translation="MSMFDTEAWKGKYRDAVQEKLPDEQVVEAWLFYRTGGYAGMALG
                     PFSPLAAIVARTIGKKRAGGLPNQFLLAVTPTKVRAFKCKPSARKAKVGNELAVWGRA
                     GLRVSVRDAAVNAEVTLESPGEGEKVVCSTGKDTASREFLALLSQPVGPQAPTPAPA"
     gene            11871..>13100
                     /locus_tag="VF517_05965"
     CDS             11871..>13100
                     /locus_tag="VF517_05965"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX8052520.1"
                     /translation="MDPPSPTGGAGVKRTRRGGARARRVAGLAGLAGLIAASLLVVAG
                     AQSGLDVLVPSAKLRYPGWLHGPLEPLDVAVSNDGLAWLLLALCASYALVLAAGTELP
                     RKPTLGAIAALHLLYVLAPPLLSSDVFGYVDLGRLGAVHGIDPYSPADTPLPADEVYA
                     YRRWGTDLPAPYGPLFVAAMYPLGLLGVPGALWAIKALLGAASLGVVALVHRAAERAG
                     RDPLAAAAFVGLNPILLMWGVGGAHNDFFFVLLLMVAVTTQSERVGGAALAAGAAVKA
                     SAGLPLVLMLAGARSRRAAVTGVLAAGAAVLVLSAAIFGTDVLRIASTLRDQQDDVAI
                     FSFPNQLGDWLGFGGITDGIRLVSVALLVGGLAWMLAQVARRRLDWVAAAGWATALLL
                     VTTAWLLPWYLVWLLPLA"
BASE COUNT         1642 a         4700 c         4750 g         2008 t
ORIGIN      
        1 ctgaccgacg ccccaggcgc cttcgctcgc gtccatgccc tagaccgtat cccgcagccg
       61 ggcgatgagc tcggcggaga gctcgccgcg gtagacgggc tctgcccagc cgctgaggtc
      121 gacgtggagg tcctccgtga cgtccacggt cagctccccg ccgtcgagca cgacggtcac
      181 cggcgagtcg ccgccgcgga cgacgtgggc gaccgccgcg ccgcacgcac cggttccgga
      241 ggacagcgtc tcccccaccc cgcgctcgaa gatccgggcg cggatgcggt tcggcgccag
      301 cttcgtccag aaggagacgt tcgtgcgctg cgggaactgc ggcgcgtgct cgatggccgg
      361 gccgatcgcc ggcagctcga ggcgctcgag ctcgtcgcgc gccgcgacgc ggatggcgca
      421 ctgcgggttg ccgatcgaga cgtgctggaa gctcagctcg ccgccgagtg cggcgagggt
      481 cccggtgccg tcctcccccc cgctcgggaa gtccttcgaa gttaggctcg cgcggcccat
      541 gtccacccgg caggtggtcg ggccggtgat ctgcggccgg atcggcccgg cggcggtctg
      601 gatcgcgaac tcgtcccggt ccacccagcc gcgctggcgc aggtagagga tcgcctcgcg
      661 cgcgccgttg ccggacagct cggcctccga gccgtcgggg ttgaatatgc gcaggcgcgc
      721 gacgaagccg tcctcgtcgg gctccgagag cagcagcacg ccgtcggagc cgacgccggt
      781 gtgcggagcg cagagccggc ggatccgccc gggggtgagc tcgaacggga gctcgctggc
      841 ctcgacgatc aggtagtcgt tgccgagggc ctgccacttc tcgaagcgca tgcggcgggc
      901 gactatacgc ggcgtgggtc gcgccacatc ggccagatgg tcggtccctc gggcagcgtc
      961 agctcctcgg tcacacggaa gccgtgacgg gcgtagaagt cgaggttgct cctcttcgag
     1021 gactcgaggt aggcgggcac ctcgttgcgg tcgcaatcgt cgagcaccgg ctgcatcagg
     1081 gcagagccga tcccgcgccc ctgcgactcc ggctcggtgc cgaggatcgc caggtaccag
     1141 tgcggctcct tggggtgggc ggcgtcgacc gcgctccacc cgcgcgcgag cagcggcgtg
     1201 cgcaggcggg tcatcggcag cacccgcatg acgaacatga ggccctcgag cggtcccatc
     1261 tcccagcgcc ccggctgcgc ccagcaggcg gcaccggagt ggtcgtcggt cgtgtagacc
     1321 tcctcctggg cgagcaggcc ggcaatgcgc aggcggaaga agcgctcgtt gagggcggcg
     1381 cggttgccgt cgtccgggaa gaaccagcgc atcacggggt cgtcgtaaaa ggcgcgcgtg
     1441 agcgtgcgca cgacctcgtc caggtcgctc ctctccgctc gccggacgct cggggcgggg
     1501 ccgatcacgc cagggtcccc gcgatctcgc gcgcgacgtc ggccggctcg cggccggtca
     1561 ggtcgaggac ggtcacgccc gcgagcttgc gcatccaggt gagctggcgc ttcgcgtagt
     1621 tgcgcgtgcg gcgcttcatc ccctccacgt ccccggccag cagctcctcg aacccgagcg
     1681 ccttgcgcgc cgtatgggcg acgcccgccg cggcggcacg gcgcacctcg tcggccgccc
     1741 cgcgcgcgac catcgcgtcc acgcgccggt cgatccggtc gtagaggtcg tcgcgatcgc
     1801 gcgtgaggcc gaacaggcgc gtgggccggc gcgtctcgcc cgtccacagc tcgctgtcgc
     1861 cggtcggcgg gggcgcctcg ccgaggtcga gcagctcgag cgagcgcacg atccgcgtgc
     1921 ggtcggtcgg cgcgacggcc gccgcggcgc gcggcgagcg ctcgcgcagc tcggcgtgca
     1981 gggcggccgg gccgcgctcg tccacggcgc gctgccagcg cgcgcgcagc tccggtggcg
     2041 gcgcgggcgg cagcgagagc tcggtgagcg cggcgcgcag gtagagcccc gtgccgccca
     2101 gcacgatcgg gcgccggccg gactcgagcg cgcggtcgat ctccgcgtgc gccagcggca
     2161 tgaactcccc ggccgagaac gactcccgcg ggtccacgaa ggcgaccagt cggtggtcga
     2221 gccgcacgcg ctccgcggcc gacgccacgc cggtgagggt ctccagcccg cgatacacct
     2281 ggagcgcgtc ggccgagatc gcgacggggt cctcgccgcg ggcgcgcagc aggtcggcaa
     2341 gctcaacggc cacggcggtc ttgccgacac cggtagggcc gaagatcgcc acgacctcgc
     2401 tcatgggacg aaccatcgca ccaccgtcag aggaccgccc cgcggaggcc gcgccacgcg
     2461 ccagcgccat cctcgaatcg cggccggtcg tcgcgctgct cggcctcgcc gcattcgcgc
     2521 aggcgctctg cttcgcggtc gtggccggcg tggtcggcct cgcgatcgtg cgcgggggca
     2581 gcgccgccgg cgatcacctg gccatcggga tcgtctggct cgtggcgctc acgctcgtgg
     2641 gtacggtgct ctgcgcgctc gcgcaagcgg ccctgatctg gcacgcgaac gagaccgacg
     2701 cgctcgccgg gaccaaccgc cggcgctggc tgatccgcct cacgatctgg ggcccgctcg
     2761 cgatgccgtc ctactggcgc cgctacatgc gccgctaggc ggcgggaagc gccggctcgc
     2821 gcacgcggtc gcgcaggacc acccgcgtgc cgcgcgaggg tcgcaccgtg atgttgcgcc
     2881 gcgccgcgac ctcccgccct ccgccgtcgg gcacgatgtc gagcgacttc agcatcgcgc
     2941 tcatgatgat cttcagctcg accatggcaa agctcgagcc gacgcagcga cggcgcccgc
     3001 ctccgaaggg gaaccacgtg taggtgccgg gtgcctcgtc gaggaagcgc tccgggcgga
     3061 acgcgtacgg gtcgtcgtag atctcggggt cgtggtggat caggtacgag ttcgggatga
     3121 ggatcacgcc ggccggatag gaccagccgc caacctcgat cggcttcatg gcgagccgcg
     3181 gcgacgccgt cgggagcacc gggcggtcgc gcagcgtctc gtagatcgtt gcggtcaggt
     3241 agtcgtcgcc gtctccggcg tcgatctcct cgcgcagccg ctcgacgacc tccgggtgct
     3301 gcggcaggcg ctcgaacgcc caggcgagcg ccgacgccgt cgtctcgtgg ccggcgacga
     3361 gcagggtcat cagctcgtcg cggatctcct ggcccgacat cggcgagccg tcctcgtggc
     3421 gcgcctcgag caggagcgag aggatgtcgt cgcggtcggc ggggtcgcgg cggcgctcct
     3481 cgatcagctc gaagatcatc ctgtcggcct cgcgctggcc gcgcttgaag gtcgcgtacg
     3541 gcccgacgcg ctcgagcagc ggcgacaggc ggttgcccgg ctgcggcggc accaggctca
     3601 cgaagctgtc gccgtaggcg agggccgcgg cgagcttgtc gcgcagggcg tcgaggcggg
     3661 gcccggggcc gagtccgaag agggagcgca ggatgatctc gagcgtgagc ttctggaagc
     3721 gtgggtggag ctcgacgggt tcgccgcggg gccagctggc caggtcctgc tctgcgacct
     3781 cccgcatgac ccccgcgagg cgctggatct tctcgccgtg gagcgccggg aggagcagct
     3841 tgcgctgctc catgtgcgga tcctcgtcga ggacgatcag cgagtacgag cccaggaccg
     3901 gctcgagcac ccgcgccacg ccggagccgg gatgcagcac gtccggcggc gccatgaaga
     3961 cctccttcac gtcgctcgga tcactcagca cgacgaacgg cggcacggtg ggcaggcgca
     4021 gcgtgaagcg cttcccgtag cgggcccggc agcgctcgaa gtacggcagc ggacggctcc
     4081 agaagccgag ggcctggatc cagccggggt agcgggggcc gggcgggatg cccgagttac
     4141 gcgacaaggt ctctcctccc gatcgtcgcc aaccggagtt ctaccggagg ctcaaacggt
     4201 ctgtgtgatg ctgcccaccc gctcgcggcc ggcgagcgtg gtgctggtgg cggagtcgat
     4261 ctccacgtcg gtgaacgagc cgggctcggc ggcgcccgcg aagtgcacgg tcttgttgtg
     4321 gcgggtgcgg ccgcgaagcc ggtcggggtc ggtccgcgac ggtccctcca ccagcacctc
     4381 catggtgcgt ccgacgaacc gctgcgcgcg ctcggtcgcg cggcgctgga ccacctcgac
     4441 caggcgctgc atgcgctcgc gcttgacggg gtacggcacc tggtcgtcga gatcggccgc
     4501 tgcggtgccg cgccgcggtg agaacacgaa cgtgaacgcc gagtcgtagc cgacctcctc
     4561 cgccaccgcg agcgtctcct cgaagtccgc ctcggtctcg cccgggaagc cgacgatgat
     4621 gtcggtggtg agcgcgcagt ccggcacgtg ctcgcggatc agggccacgc ggtccatgta
     4681 gcgaccgcgg tcgtaggtgc gccgcatcgc cttgaggatg cggctcgagc ccgactggag
     4741 cggcaggtgg atgtgctcgc agagcgcggg cagctccgcg tgggcgcgga tcacgtcctc
     4801 gcgcatgtcc ttcgggtgcg ggctcgtgta gcggatgcgc tcgatgccgt cgacggcgtc
     4861 gaccatcgcc agcagctcgg cgaaggtggc cttcgcgtcc ctcgggaggt cgcggccgta
     4921 ggagttcacg ttctggccga gcagcgtcac ctcgctcacg ccgtcggcgg ccaggcgctc
     4981 cacctccgcg accagctcgg ccgccgggcg gctgacctcg cgcccgcgcg tggacggcac
     5041 gatgcagtag gagcagacgc agttgcagcc caccgagatc tgcacccagc cctggaagtc
     5101 gcgggcgcgc ttgggcggca ggtggccggt gaacccctcg aactcgaagt agccctgcgc
     5161 gctgaggctg tcgctcgtca ggaactccgc gagcttcggg acctggccgg ggccgaaggc
     5221 gacgtccacg aacggaaagc gctcgaacac ctcgtccttg atcgactgcg cccagcagcc
     5281 gccgacgccc acgatccggt ccgggttctc ggtcttcagg cgcttggcct cgccgagatg
     5341 gccgatcagc cggttgtcgg ccgactcgcg gatcgagcag gtgttgaaga ggatcaggtc
     5401 ggcgccgtcg cgctccggaa cctcgctgta gccgagcgac tcgagcatgc ccttcatccg
     5461 ctcggagtcg tgctcgttca tctggcaccc gaaggtggtc acgtggtagg tcctgctcac
     5521 ggcgttcagc ctagccgcgt ggagccgtcg ggctcgatgc tccagcgcgg gttgtgcgcg
     5581 acctcccacg ggtgcccgtc ggggtcgacg aacatgcccg agtagccgcc ccagaaggtc
     5641 tcgccgggct cgcgccggat ctctccgccg gcggcccgcg cctcctcgat cacgctgtcg
     5701 acctcctctg gggagcgcgt gttgtaggcg agcgtgattc cgccccagcc gcccgagtcg
     5761 gtcacgccgc tgtcctcggc gagctggtcg cgaccccaga gcgccacgac catgccgacc
     5821 gcctggaaga agaccacgtc gtcgtccggc cccgcgttcg tgtgccagcc gagcgcctcg
     5881 tagaacgcgc gggcccgcgg gaggtcgcgc gtgccgagcg tgatcaggct gaggcgctgg
     5941 tccacgcccg cattatcgcg ggtcggccgg ccgctacgac ccccagttgt acagacccac
     6001 ctcggcctcg tttccggttc cgtacttgta gctcacgagg aaccagcccc agaggttctc
     6061 tccgtccggg ttggtcaggt gcacgtaggc ccagcgcgta tagccgttga tcgtgttggt
     6121 ctggtacgag tacgagttga gcacctgctg cgacacgtcg caggcgtaca catgcgtgtt
     6181 cggcccgatg ttggccatga tggctccacc gccggggagg acggcctcgc tcgccccggt
     6241 gtccctgcgc ggcatgttcc agaggatgta gttgtacgag tcgccgtggt cgtccttgcc
     6301 ggggtcgccg tagttggccc atgacgaccc ggatccgact ccctcgctca ggctcttgta
     6361 gaacatgtca cccggcatcg acaccggctt cgaggtgagg tggaggttgc aaccgggtgc
     6421 cgagcgcccg ttgcccatgc gcgcggagta cggcaggagg tcgggcggcg gattgagctc
     6481 gctgccggcc acgtgggcgt gggcctggct gtagtcgccg ccgccgcggt tcatgtacgt
     6541 gagtcgcccg ccgatcgtga gacgctggag ggtggagatg cggagccggt tcggtccgca
     6601 ctccggcgcg cgagtcgtcc cgatctggaa cggcagcagc cggccgccga agccgtagat
     6661 cgggatgttc gccgcggcag gggcgcgggt ggtgtagacg caggtgctca ccgggacctg
     6721 atagcgggga tcgtcgggcc gcttggcgaa ctcgttgttg tagtcccagc cgttgtcggt
     6781 ggcgcccgcg ggcgcggcca gggctgccat cgccacgacc gtaagcagtc cgatcgagcg
     6841 cttcctgccc atgtgccacc cctatccgtg ccgagtccga gtcgattccg accctaaccg
     6901 gtttggtgca cccggcgcaa gcgctcagcg ctcccttaac gatgagggct caggagagtc
     6961 gccagaacgc gccggtcagc ccgacggcgt cgagggtcgc ctgcgactca ggcgtggtgc
     7021 gcgggcgcca gtccggatcg agccggttgc cgtaccagcg cttggccagc gcgttaagcc
     7081 ggccgaccgg catcgccgcg ccatccgtcg gggtctcctc ctccgaccgg aagagccgca
     7141 tcgtgcgtca ggtgtgggcg atgtcgtccc accagttgcg ggccggcacg gcgacgtgga
     7201 agacggggcc cggctcgacc tcgccgctct ccacctcgag cgtgactccc gtgctggtga
     7261 cggacgctcg ctcgagggcg agcgccgcga cgatgccgag gccgtcccac gcgcagctgc
     7321 cccaccacgt gcggcctccg gactcgacct tcgcgtccgc gtgaggggcc gcgaacgggt
     7381 gcgccatcac gatccggtcg cggtcgtcga gcacgacgac gtgctgctcg gcgagcgagt
     7441 ggagggtggc gcggtcgccg ggtggctcgg gcggctcggg cggagcgccg gtcgccgcga
     7501 acgtccgcat gatctccgcc cgcagggcgt gctcgcgctc ggtcatctac ttcgcgaagt
     7561 cgacagagcg gcgctcgcgg atcacggtca ccttgatctg tccggcgtac tcgagctcgc
     7621 gctccacgtc gcgcgcgatg cggtgcgaga gcagcgcggc ggtgtcgtcg tcgacctcct
     7681 ccggatgcac gatcacgcgg atctcgcggc ccgcctgcat cgcgtagacc ttctcgacgc
     7741 ccgtgtggcg gccggcgatc tcctcgaggt tgcgcaggcg cttggtgtac tgctcgagcg
     7801 actcgccgcg cgctcccggg cgggcgccgg acagcgagtc gacgatctgc acgatcaccg
     7861 cctcgatcgt gcgcagctcg acctcgttgt ggtgggcctc catcgcgtgc gcgacggcct
     7921 cggactcgcc gtagcgccgc gccagctcgc cgccgaccag ggcgtgcgga ccctcgatct
     7981 cgtgcgtgac ggccttgccg atgtcgtgga gcagggccgc gcgcgcggcg cacttggggc
     8041 tcgcgcccag ctcggccgcg agcaggcggg cgagccgcgc gcattcgagt gagtgggcga
     8101 gcacgttctg gccgtagctc gtccggtagt ggaggcgacc caggagcttg gcgagctcgg
     8161 ggtgcacggt gccgatgtcg gcgtcgagca ccgcctgctc ccccacctcg acgatgtggc
     8221 tctccatctc ggtgcgcgcg gtttcgtatg cctcctcgat gcgcgcgggg tggatgcggc
     8281 cgtcctggag gagtctctcg agcgtgagcc gggcgatctc gcgacggacg ccgtcgaagc
     8341 ccgacaggag cacggcaccg ggggtgtcgt ccacgatgaa gtcgatcccg gtgagcgtct
     8401 ccagcgcgcg gatgttgcgg ccctcgcgcc cgatgacgcg gcccttgagg tcgtcggagt
     8461 cgaggtgcac gaccgagacg gtggtctcgg ccgcgtggcc gccggccacg cgctgcatga
     8521 cggtcgagag gatgctccgc gcgcgctggt cggcctcgcg cctggcctcc tgctcgacct
     8581 ggcggacgag cgcggcactg tcgtggcgca gctcctcctc gagctcgcgc aggaggatct
     8641 ggcgggcctg gccggcgctg aggcccgcga tgcgctcgag ctcgcggagc tggtcgcgct
     8701 tggccgcctt gagcttctcg gcctcgtgct cgaggttgcg gcggcggtcc tcgagggcgg
     8761 tctcgcggcg ctccaggtcg gccaggcgca gatcgaccga ctcctccttg cggagcgtcc
     8821 gctcctcgat tcgagcgatc tccgcgcggc gctcgcgcag ctcggcctcg atgtcggcgt
     8881 gccgggcgcg caggtccgac tcgaggtcgc ggcggatggc gtcgatgtcg gcggacggag
     8941 ccggcgccgg gtgagtcgcg ccgggttgct cgccggtctc gcggttgaag tccccggtct
     9001 cccgggtgaa gcgccctggc ggatgctcgg ggctcgcggc ttcgaccccg gcggcggcct
     9061 gtgtaacgct cgcctcgcgc cgctgggggg cgggggccgt cgcacccgag tccggggcgg
     9121 gggcgggcgc gcccgccgac ctcgggtccg gcgctggggc gccgggtccg gctccgggtc
     9181 ccgccccggc tccggcggct gcggccccgg gcgcgtggcc ggcggctccg gccccgggcg
     9241 cgtggccggc ggccgtgatc atccgcggac gcaggagcag caccaccagc gccgacactc
     9301 cggccgacac gactgctgcg agcacgatct ccatatctcc gctctcccac tccggcgcac
     9361 tgcaccggcc gtcgatcgct gtcggcgacg tggctcggct gccgcaagac ccagtctcgc
     9421 gctatgggcg ggtgggcgca gagcgcgccc ggagacgtcc tacgggtgcc ctgtctgcct
     9481 cctccggggg cgatcccgcg tcgattggcg aatgaaggtc ttcaaggtct cgctggctgg
     9541 ttcccgcatc aagaaaagcg caatttgctg gtatttcgtt ctcgcggcgg ccggtcaggc
     9601 cgctgctctc aaagttaccg ctgggggcaa gggggcgcaa cttctttgcg ctgctggggc
     9661 ggcgtgcgtc tcgttcttct cgggggtacg ccgctgcgcg gctcagagag cccccctcga
     9721 agcacgagcc gcacgccgca cgatcgccgc gggtggctcg ggcaaacgtc cgtaggtacg
     9781 gacggcgtgc accgcgccgc cgcagcgctc tatgccgggc tgcggcggcc tagggttgag
     9841 ccaagcggcc tgctctgcgc tgctcggccc gcgctacgcg gcctgctcgg ccggcgctac
     9901 gccgcctgtg gtcggctccg gtacgggctc ggtggcctgg ccgttgccgt cgcccacgat
     9961 ctcgtccggt ggcgggaccg ccgccagcgg acgggcgggc tcgatgccca gggccgcgta
    10021 gatcttcgcc tcgatctcct tggccatggc ggggttctcg cgcaggaagc ccttggcgtt
    10081 gttgcgcccc tggccgatgc gctcatcgcc gtaggagaag aacgagccgg acttctggat
    10141 caggttgtgc tcgagcccga ggtcgatcag gcagccctcg gtggagatgc ccttgccgta
    10201 ctcgatgtcg aactcggcct ggcggaacgg cgccgcgacc ttgttcttga cgaccttcac
    10261 ccggacccgg ttgccgacgg cctcggtgcc gtccttcaac gtctctatac ggcggatgtc
    10321 gagccgctgc gacgagtaga acttgagcgc gcggccaccc ggctgcgtct ccggcgaacc
    10381 gaacatcacg ccgaccttct cgcggatctg gttcgtgaag atgcagagcg tgttggcacg
    10441 gttcaggttg cccgccagct tgcgcatggc ctgcgacatc atccgcgcct gcaggccgac
    10501 cgtctggtcg cccatcgcgc cctcgagctc ggccttcggc gtgagcgccg ccaccgagtc
    10561 gatcgccacg acgtcgacgg cgccggagcg gatcagcagg tcggcgatct cgagcgcctg
    10621 ctcgccgtgg tcgggttggg agacgagcag ctcgtcgacg tcgacgccga tgcgcttcgc
    10681 gtacacgggg tccatggcat gctcggcgtc gatgaacgcg cagatgccgc cggtcttctg
    10741 agcctccgcc agcaggtggt agagcagcgt cgtcttaccc gaggactccg ggccgaagac
    10801 ctccacgatg cgcccgcgcg ggattccgcc gacgcccagg gcgaggtcga gcgacagggc
    10861 acccgtcggg atcgcggcga cctggacctg gaactgctcg tcgcccatct tcatgatcga
    10921 gcccttgccg aactggcgct cgatctggcc gagcgcgccg ttcagcgcgg cgtcgcgggc
    10981 cttggccgcc ttctcctgct ccttgtcctt gtccatctct ggctcctctg tctctctgag
    11041 tcgacagaag ctaaaggtgg gatcggacgg cttttcgccc ccctggggtg cgaagccgcg
    11101 cggcgggcgg agccgggggg ccggggcgaa gccgcgccgg gagccggcga gccgaggcga
    11161 gcgccggcga gtcccgcggg ccgagccggg cgcgcgccgc gcatcgattg gagggttatg
    11221 ccgccatagg cgggccaagg ctccaatcga tgagaaaccg ctcgatcctt cccccttagg
    11281 ggctactcga tgaccgcgag ccccgtcgcg agccccgcta tcgctatggc cagcacgagc
    11341 gcgatgccga cccaggccca ccaggtgccg ctgccgcggc gcttgtggat ggtcacgcgg
    11401 gcgcgggagt cggtgcctgc gggccgaccg gctgcgagag cagcgccagg aactcgcggc
    11461 tcgccgtgtc cttgccggtg ctgcacacga ccttctcgcc ctcgcccggc gattcgagcg
    11521 tcacctcggc gttcaccgcc gcatcgcgga cgctgaccct caggccggcg cggccccaga
    11581 cggcgagctc gttccctacc ttggccttgc gcgccgaggg cttgcacttg aacgcgcgca
    11641 ccttggtcgg cgtcacggcg agcaggaact ggttcggcag gccgccggcg cgcttcttcc
    11701 cgatcgtgcg ggcgactatc gccgccagcg gcgagaacgg accgagcgcc atgcccgcgt
    11761 agcctccggt ccggtagaag agccacgcct ccacgacctg ctcgtcgggg agcttctcct
    11821 gcacggcgtc gcggtacttg cccttccagg cctctgtatc gaacatggac atggacccac
    11881 cctccccgac cggcggcgcc ggagtcaagc gaacccggcg tggcggcgct cgcgcgcgac
    11941 gtgtagccgg cctcgccggc ctcgccgggc tgatcgccgc gtcgctgctc gtcgtcgcgg
    12001 gcgcccagtc cgggctcgac gtgctcgtcc cgtcggcgaa gctgcgctac cccggctggc
    12061 tgcacggacc gctcgagccg ctcgacgtcg cggtgagcaa cgacggcctc gcctggctcc
    12121 tgctcgccct ctgcgcctcc tacgcgctcg tactcgccgc gggcacagag ctcccccgca
    12181 agcccaccct gggcgccatc gcggccctcc acctcctcta cgtgctcgcc ccgccgctgc
    12241 tctcgagcga cgtcttcggc tacgtggacc tcggccggct cggcgccgtg cacggcatcg
    12301 acccgtactc ccccgccgac acgccgctcc ccgcggacga ggtctacgcc taccgccgct
    12361 ggggcaccga cctccccgcc ccctacggcc ccctcttcgt tgccgccatg tacccgctcg
    12421 gcctgctcgg cgtgccgggc gcgctctggg cgatcaaggc cctcctcggc gccgcgagcc
    12481 tcggcgtcgt ggccctcgtc caccgcgcgg ccgagcgcgc cgggcgcgac ccgctcgccg
    12541 ccgccgcgtt cgtgggcctc aacccgatcc tgctcatgtg gggcgtcggc ggcgcgcaca
    12601 acgacttttt cttcgtcctg ctgctgatgg tcgcggtgac cacgcagagc gagcgcgtcg
    12661 gcggggccgc gctggccgcg ggcgccgcgg tcaaggcgtc ggcgggcctc ccgctcgtgc
    12721 tcatgctcgc cggcgcacga tcgcgccggg cggcggtcac cggcgtcctg gcggcgggcg
    12781 cggcggtgct cgtcctgagc gccgcgatct tcggcacgga cgtgctcagg atcgcgagca
    12841 ccctgcgcga ccagcaggac gacgtcgcga tcttcagctt cccgaaccag ctcggcgact
    12901 ggctcggctt cggggggatc acggacggga tccgcctggt gagcgtcgcg ctgctggtcg
    12961 ggggactggc ctggatgctg gcgcaggtgg cgcgcaggcg gctcgactgg gtcgccgccg
    13021 ccggctgggc caccgcgctt ctgctcgtca cgaccgcctg gctgctgccg tggtacctcg
    13081 tctggctgct cccgctcgcg
//
LOCUS       DASVPL010000119         7327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_93893, whole genome shotgun sequence.
ACCESSION   DASVPL010000119 DASVPL010000000
VERSION     DASVPL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93893"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VF517_05970"
     CDS             complement(<1..608)
                     /locus_tag="VF517_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX8052521.1"
                     /translation="MRRSLARLSIPFREPFVTSAGVVPARELVLLRLEEDGVVGYGEA
                     APFEPYDGVPIEAAVEALSNGDGNGPPQARAAEEMARLDLEARLAGRSVGEPGAEAIA
                     VNHTLPAGPPDEVARRAAEGVRAGFSCFKLKVGLPDDAERVAAVRAAIGPWPALRVDA
                     NGAWSAPEAVRVIEELAVHDLQLVEQPCRTVEELAEVRRRVPV"
     gene            complement(605..1486)
                     /locus_tag="VF517_05975"
     CDS             complement(605..1486)
                     /locus_tag="VF517_05975"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX8052522.1"
                     /translation="MSPLRLWLVAARPRTLPAAVAPVLVGTALAASEDVFKLDRFVAA
                     VIASVFVQIGTNLANDYSDARRGADTDERLGPVRVTAGGLMPPQRVLTGTYIAFGIAL
                     AAGVYLSAVAGWELMLVGIASVAAGALYTGGPRPYGYEGLGELFVFLFFGVVAVGGSY
                     FVQTEDLRWEAFALAVPVGLLASAILVVNNIRDIDTDRRASKRTLAVKLGRDRSRGLF
                     AAMVYLTFVVPVVVAAASDLDWWLLLALGALPLAPPLVRTVRTRTDGTALNGALAATG
                     RLLAVYSVLLAAGVLLS"
     gene            complement(1529..2395)
                     /gene="menB"
                     /locus_tag="VF517_05980"
     CDS             complement(1529..2395)
                     /gene="menB"
                     /locus_tag="VF517_05980"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX8052523.1"
                     /translation="MEQSTDERAIRPGADWQPAGDYGDIRYETSDGIAKITIARPEVR
                     NAFRPQTLIEVSDALDRAREDLDIGVIVLTGEGPLAFCSGGDQRVRGDTGYMAEDEAA
                     QRGVGRFHVTDLHVQMRRLPKPIVAMVAGYAVGGGHVLHVVCDLTIAADNARFGQTGP
                     KVGSFDGGFGASVLADLVGTKKAKEIWFLCRQYGADEALAMGLVNKVVPLEELEEETV
                     RWCREMLELSPFALRLLKASFHAAEDGYAGIQQLAHDTNLLFYGNEEAQEGRNAYREK
                     RKPDFGKFPRRA"
     gene            2394..3611
                     /locus_tag="VF517_05985"
     CDS             2394..3611
                     /locus_tag="VF517_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8052524.1"
                     /translation="MRAYDKALDDWISLRACTHPDHPAVVAEGRSVTYAELDASLGPA
                     VAAVPARGTHEFARQLWSAWREGTAFVPYDPRLPGPPAAVDAGPEVRTVIFTSGTTGQ
                     PKQVGLTAANHEASALASAWNLGVDPGDRWLCCLPLWHVGGLSILHRSAIYGTTAVLH
                     DGFDAARVRESLESGEATVVSLVATMLRRLIDAGLSEWPALRAALVGGGPVPRELLEW
                     ARASGFPLLQTYGMTETCSQVATLPAAEALAKAGSAGRPLVGVELRIGAGGQILVRGP
                     MVAPGAADGDGWLHTGDRGRLDGDGFLHVEGRIDDVIVTGGENVAAAEVEAALAAHPA
                     VADAAVAGRPDPEWGEAIVAYVTGASDVTDAELLAHCRERLERHKLPKAIVRVAELPR
                     NAAGKVVKAALPH"
     gene            complement(3683..5647)
                     /locus_tag="VF517_05990"
     CDS             complement(3683..5647)
                     /locus_tag="VF517_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052525.1"
                     /translation="MTRTLRRALAAIPLAAALLAALPAMASAAATLTVTAGSYDYDSG
                     AGVANRLSVSETTANPFITFTETTETITVAGPNAATCAGSGTMNVTCATVPTAVDVNL
                     GDLDDKLAYNAFSVASHSVTVDTGTGNDSELNVGSSLVQFTTNSATEPPITVNGNDGD
                     DNLFVNAGNNIGQGTATIVNGGNGNDLLKNANGATLNGDANNDTFVPSNPGGGSEFSE
                     TMNGGAGEDIADYSGFTTGVNVSVDAVANDGPTTGQTDNVGADSSVENVRGSGGADTI
                     TGTLANQNNKFFGGSANDTIDGGPGDDVLDGEQGADTMNGGANGATGDTVSYAARSGS
                     VTADNDGVADDGENGLVVPTNPGQPPASPQNEGDNVGATVENITGGSGNDSLTGDGAA
                     NALTGNGGDDTLDGNAGADTLGGGAGDDDLDGAADGDTINGDSGDDTADGGAGTDTVN
                     GGNGFDRLDGGAGTDTVNGDAGGDTIGAMDGAADTVNCGTGTDNVLPDASGDTVNADC
                     EISGSGGSGGLGPAGPAGPAGPQGSKGEQGSPGSKGDQGPAGAQGMAGPQGPQGNPGQ
                     DVSVGCQVEPNSAKTRLAVVCDVFRTGSSRPIVAKLTRGSKTFGRGSSKIRNNTVRFN
                     LRTSKLSKGRYRLTLTQKGVRLITLGVTIR"
     gene            5787..6776
                     /gene="ilvC"
                     /locus_tag="VF517_05995"
     CDS             5787..6776
                     /gene="ilvC"
                     /locus_tag="VF517_05995"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX8052526.1"
                     /translation="MIYDNDADLSKLDGKTVAILGYGSQGHAHALNLKESGVNVVVGL
                     REDSSSVEKARGDGLEVLPIADAASRGDVVMILLPDEKQGAVWESDIADGIAPGNLLM
                     FAHGFSITYDQIDPGPEVDIGMVAPKSPGHLVRRQFVEGNGVPGLIAVERDASGDARE
                     MVLAYAKGIGCTRAGVIETTFREETETDLFGEQSVLCGGVTELVRAGYETLVEAGYDP
                     RLAYFECLHELKLIVDLMYEKGITGMRYSVSNTAEYGDLTRGKRVIGEPARQAMKEIL
                     AEIQSGQFAREWIAENQAGQENFQRMREEQKGHQIEREGRELRSMMDWIETEF"
     gene            6862..>7327
                     /locus_tag="VF517_06000"
     CDS             6862..>7327
                     /locus_tag="VF517_06000"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8052527.1"
                     /translation="MKIVIKQGPNSGEELEVQGSIVVGRDPEAASFVIDDPEASRRHA
                     SIVPSETGVVVEDLASTNGTFVNGERIEGTRDVGAGDEIRIGNTVLEVQSAVEVTRMS
                     PIPEASDPDATAIGVAIPDEPPPAPPAATEPPAAPEPPAPEPPTAAEPPPAPA"
BASE COUNT          960 a         2665 c         2665 g         1037 t
ORIGIN      
        1 accggcaccc ggcggcgcac ctcggcgagc tcctcgaccg tccggcaggg ctgctccacg
       61 agctggaggt cgtgcacggc cagctcctcg atcacccgga ccgcctcggg cgcgctccag
      121 gcgccgttgg cgtccacccg cagcgcgggc cacggtccga tggcggcgcg aacggcggcc
      181 acgcgctcgg cgtcgtcggg cagcccgacc ttgagcttga agcacgagaa ccccgcgcga
      241 accccctcgg cggcccgccg cgcgacttcg tccggcggcc cggccggcag cgtgtggttg
      301 accgcgatcg cctcggctcc gggctcgccc acagaccgcc cggccagccg cgcctcgagg
      361 tcgagccgcg ccatctcctc ggccgctcgc gcctgcggcg ggccgttccc gtcgccgttc
      421 gagagcgcct cgaccgcggc ctcgatcggc acgccgtcgt agggctcgaa cggcgccgcc
      481 tcgccatagc cgaccacgcc gtcctcctcg agcctcagca gcacgagctc gcgcgccggc
      541 accacccccg cggaggtgac gaacggctcg cggaacggga tcgacagccg cgcgagcgag
      601 cgcctcacga cagcagcacc ccggcggcga gcaggaccga gtacacggcg agcaggcggc
      661 cggtggcggc aagggcgccg ttgagcgcgg tgccgtcggt gcgcgtgcgc accgtccgca
      721 ccagcggcgg ggcgagcggc agcgccccga gcgccagcag cagccaccag tcgaggtcgc
      781 tcgccgccgc gacgaccacg ggcacgacga acgtcaggta gaccatcgcc gcgaacagcc
      841 cacgcgagcg gtcgcgaccc agcttcaccg cgagcgtgcg cttgctcgcg cgccggtcgg
      901 tgtcgatgtc gcggatgttg ttcaccacga ggatcgccga cgccagcagg ccgaccggca
      961 ccgccagcgc gaacgcctcc cagcgcagat cctcggtctg cacgaagtac gagccgccca
     1021 cggcgacgac cccgaagaac aggaagacga acagctcgcc gaggccctcg tagccgtacg
     1081 gtcgcggccc gccggtgtag agcgcgcccg cggccaccga ggcgattccc acgagcatca
     1141 gctcccagcc cgcgaccgcg ctcaggtaga cgcccgcggc gagcgcgatg ccgaaggcga
     1201 tgtaggtgcc ggtcaggacg cgctgcggcg gcatcagccc gccggcggtc acccgcaccg
     1261 gcccgagccg ctcgtcggtg tcggccccgc ggcgggcgtc cgagtagtcg ttggcgaggt
     1321 tcgtcccgat ctggacgaag acgctcgcga tcaccgcggc cacgaagcgg tcgagcttga
     1381 agacgtcctc gctcgcggcg agggccgtgc ccacgagcac cggcgccacc gcggccggca
     1441 gggtccgcgg ccgcgccgcc accagccaga gccggagggg gctcatcgcc tcacgcgcgc
     1501 tcgccgcctc acgcgcgcct ccgccgcctc acgcgcgccg ggggaacttc ccgaagtccg
     1561 gcttgcgctt ctcgcggtag gcgttgcggc cctcctgcgc ctcctcgttg ccgtagaaca
     1621 ggaggttggt gtcgtgagcg agctgctgga tcccggcgta gccgtcctcg gccgcgtgga
     1681 agctcgcctt cagcagccgc agcgcgaacg gcgagagctc gagcatctcg cggcaccagc
     1741 gcacggtctc ctcctcgagc tcctcgagcg gcaccacctt gttcacgagc cccatcgcga
     1801 gcgcctcgtc cgcgccgtac tggcgacaga ggaaccagat ctccttggcc ttcttggtcc
     1861 cgacgaggtc ggcgagcacg ctggcgccga agccgccgtc gaacgagccg accttcggcc
     1921 cggtctggcc gaagcgggcg ttgtcggccg cgatcgtgag gtcgcacacg acgtgcagca
     1981 cgtgcccgcc gcccaccgcg tagcccgcca ccatggccac gatcggcttg ggaaggcgac
     2041 gcatctgcac gtggaggtcg gtcacgtgga agcgcccgac gccgcgctgg gccgcctcgt
     2101 cctcggccat gtagccggtg tcgccgcgca cgcgctggtc gccgcccgag cagaacgcga
     2161 gcgggccctc gccggtgagc acgatcaccc cgatgtcgag gtcctcgcgc gcgcggtcga
     2221 gcgcgtcgga gacctcgatc agcgtctgcg ggcggaacgc gttgcgcacc tcgggccggg
     2281 cgatcgtgat cttcgcgatg ccgtccgagg tctcgtagcg gatgtcgccg tagtcgccgg
     2341 cgggctgcca gtcggccccc gggcggatcg cccgctcgtc ggtggattgc tccatgcgcg
     2401 cctacgataa agccctggac gactggatca gtctgcgggc gtgcacccac cccgaccacc
     2461 cggccgtcgt cgcggagggg cggagcgtga cctacgccga gctcgacgcg tcactcggcc
     2521 cggccgtcgc cgcggtgccc gcgcgcggaa cccacgagtt cgcccggcag ctctggagcg
     2581 cgtggcgcga gggcacggcg ttcgtcccgt acgacccccg gctccccggg cccccggctg
     2641 cggtggacgc cggaccggag gtgcgcacgg tcatattcac ctcgggcacc accgggcagc
     2701 cgaagcaggt cggcctgacc gcggccaacc acgaggccag cgcgctcgcc tcggcctgga
     2761 acctgggcgt ggaccccggc gaccgctggc tgtgctgcct gccgctctgg cacgtcggcg
     2821 ggctgtcgat cctccaccgc tcggcgatct acggcaccac ggcggtcctg cacgacggct
     2881 tcgacgccgc gcgcgtgcgc gagtcgctcg agtcgggcga ggcgacggtc gtctcgctgg
     2941 tcgcgacgat gctgcggcgc ctgatcgacg ccgggctgtc ggagtggccc gcgctgcgcg
     3001 ccgcgctggt cggcggcggg cccgtgccgc gcgagctgct cgagtgggcg cgggcgagcg
     3061 gcttcccgct gctccagacc tacgggatga ccgagacctg ctcgcaggtg gccaccctcc
     3121 cggccgccga ggcgctcgcc aaggcggggt cggccgggcg gccgcttgtg ggcgtcgagc
     3181 tgcgcatcgg cgccggcggc cagatcctcg tgcgcggccc gatggtggcg ccgggcgcgg
     3241 ccgacgggga cggctggctc cacaccggcg accgcggccg gctcgacggc gacggcttcc
     3301 tccacgtcga gggccggatc gacgacgtga tcgtgaccgg cggcgagaac gttgccgccg
     3361 cggaggtgga ggcggcgctg gccgcgcacc ccgcggtcgc ggacgcggcc gtcgcggggc
     3421 gcccggaccc ggagtggggg gaggcgatcg tcgcctacgt cacgggcgcg agcgacgtga
     3481 ccgacgccga gctgctcgcg cactgccgcg aacggctcga gcgccacaag ctgccgaagg
     3541 cgatcgtgcg cgtggccgag ctgccgcgca acgccgccgg caaggtggtc aaggcggcac
     3601 tgccccactg aacgcgacga gcccccccgc caagggggac gggagggctc gtgcgcactg
     3661 ctctggcgcc tggcgccggg cgctagcgga tcgtcacgcc cagcgtgatc agccggacgc
     3721 ccttctgggt gagcgtgagc cggtagcggc ccttgctcag cttgctggtc ctcaggttga
     3781 accgcaccgt gttgttgcgg atcttcgagc tgccgcggcc gaacgtcttg ctgccgcggg
     3841 tgagcttcgc gacgatcggt cgcgagctcc cggtccggaa cacgtcgcag accacggcga
     3901 gccgggtctt ggcgctgttc ggctccacct ggcagccgac gctgacgtcc tggcccgggt
     3961 tgccctgcgg gccctgcggg ccggccatgc cctgtgcacc ggccggaccc tggtcgccct
     4021 tcgacccggg cgagccctgc tcgcccttgg agccctgcgg ccccgcggga ccggccgggc
     4081 cggccgggcc gaggccgccg ctgccgccgg agccgctgat ctcgcagtcg gcgttgacgg
     4141 tgtcgcccga cgcgtccggc aggacgttgt cggtgccggt gccgcagttc accgtgtcgg
     4201 cggcaccgtc catcgcgccg atggtgtcgc cgccggcgtc gccgttgacg gtgtcggtcc
     4261 cggccccgcc gtcgagccgg tcgaacccgt tgccgccgtt gacggtgtcg gttccggctc
     4321 cgccgtcggc cgtgtcgtcg ccggagtcgc cgttgatcgt gtccccgtcg gccgcgccgt
     4381 cgaggtcgtc gtcaccggct ccgccgccga gtgtgtcggc gccggcgttg ccgtcgaggg
     4441 tgtcgtcgcc gccgttgccg gtgagagcgt tggccgcccc gtctcccgtg agggagtcgt
     4501 tgccggaccc gcccgtgatg ttctccacgg tcgccccgac gttgtcgccc tcgttctgcg
     4561 ggctggcggg cggctgaccc ggattggtcg gcacgaccag gccgttctcg ccgtcgtcgg
     4621 ccactccgtc gttgtcggcc gtgaccgagc ccgaccgagc ggcgtagctc accgtgtcgc
     4681 ccgtcgcgcc gttggcaccg ccgttcatgg tgtccgcgcc ctgctcgccg tcgaggacgt
     4741 cgtccccggg cccgccgtcg atcgtgtcgt tcgcactgcc gccgaagaac ttgttgttct
     4801 ggttggcgag cgtgcccgtg atcgtgtcgg ccccgccgct cccccggacg ttctcgacgg
     4861 agctgtccgc cccgacgttg tcggtctggc cggtggtcgg accgtcgttc gcgacggcgt
     4921 ccacgctgac gttgactccg gtcgtgaacc cgctgtagtc ggcgatgtcc tcgcccgcgc
     4981 cgccgttcat ggtctccgag aactccgagc caccgccggg gttcgagggg acgaacgtgt
     5041 cgttgttcgc gtcgccgttg agcgtcgcgc cgttggcgtt cttcagcagg tcgttgccgt
     5101 tcccaccgtt cacgatcgtg gcggtgccct ggccgatgtt gtttccggcg ttgacgaaga
     5161 ggttgtcgtc accgtcgttt ccgttcaccg tgatcggcgg ctccgtcgcg ctgttcgtgg
     5221 tgaactggac gagcgaggat ccgacgttga gctcggagtc gttgccggtc ccggtgtcca
     5281 ccgtcaccga gtggctggcc acgctgaagg cgttgtaggc cagcttgtcg tcgaggtcgc
     5341 cgaggttgac gtcgacggcg gtcggcacgg tggcgcaggt gacgttcatc gtgccgctac
     5401 cggcgcacgt cgccgcgttc gggcccgcca ccgtgatggt ctcggtcgtc tcggtgaacg
     5461 tgatgaaggg gttcgccgtg gtctccgaga cggacagcct gttggcgacg cccgcgccgg
     5521 agtcgtagtc gtagctgccg gccgtgaccg tcagcgtcgc cgctgcggac gccatggcgg
     5581 gcagcgccgc cagaagcgca gcggcaagcg gaatcgccgc gagcgcgcgg cgaagcgttc
     5641 gagtcattca atcctcctat tgggcggttc ggttcgtgct gtttgggttc gcgccgaacc
     5701 agcgagtgtg ggtcggggta accctaaccg gggatgtggc ggaagcgcaa gtgcgtccgc
     5761 aaaacacggc gctagagtcc cgcgccgtga tctacgacaa cgacgcagac ctctccaagc
     5821 tcgacggcaa gacggttgcg atcctcggct acggctcgca gggacacgct cacgcgctca
     5881 acctgaagga gtcgggcgtg aacgtcgtgg tggggctccg cgaggactcg agctcggtcg
     5941 agaaggcgcg cggcgacggc ctcgaggtgc tcccgatcgc ggacgccgcc agccgcggcg
     6001 acgtggtgat gatcctcctg cccgacgaga agcagggcgc ggtctgggag tccgacatcg
     6061 ccgacggcat cgcccccggc aacctcctga tgttcgccca cggcttctcg atcacctacg
     6121 accagatcga tccgggaccg gaggtcgaca tcggcatggt ggcgcccaag agcccgggcc
     6181 acctggtgcg gcgccagttc gtggagggga acggcgtgcc gggcctgatc gcggtcgagc
     6241 gcgacgccag cggcgatgcc cgcgagatgg tcctcgccta cgcgaagggc atcggctgca
     6301 cccgagcggg cgtgatcgag acgaccttcc gcgaggagac cgagaccgac ctgttcggcg
     6361 agcagtccgt gctctgcggc ggggtgaccg agctggtccg cgccggctac gagacgctcg
     6421 tcgaggccgg ctacgacccg cgcctcgcct acttcgagtg cctacacgag ctcaagctga
     6481 tcgtggacct gatgtacgag aagggcatca ccggaatgcg ctactcggtc tcgaacaccg
     6541 ccgagtacgg cgacctgacg cgcggcaagc gcgtgatcgg cgagccggcg cggcaggcga
     6601 tgaaggagat cctcgccgag atccagtccg gccagttcgc acgcgagtgg atcgcggaga
     6661 accaggcggg gcaggagaac ttccagcgca tgcgcgagga gcagaagggc caccagatcg
     6721 agcgcgaggg ccgcgagctg cgctcgatga tggattggat cgagacggag ttctgaggcc
     6781 ggtccccgga ctggggacac gcgccgcgcc ggcgcggcca cgtgctggcg atccgccggg
     6841 tgagcggtta gggtccggcc gatgaagatc gtcatcaagc aggggccgaa ctccggtgag
     6901 gagctcgaag tacagggttc gatcgtcgtg gggcgcgacc ccgaagccgc ctcgttcgtg
     6961 atcgacgatc cggaggcctc gcgccgccac gcgtcgatcg tgccgagcga gaccggcgtc
     7021 gtggtcgagg acctcgcgtc gacgaacggc acgttcgtga acggcgagcg gatcgagggc
     7081 acgcgcgacg tcggcgcggg cgacgagatc cggatcggga acaccgtgct cgaggtgcag
     7141 agcgccgtcg aggtcacgcg gatgagcccg atccccgagg cctccgaccc cgacgcgacc
     7201 gcgatcgggg tggcgatccc ggacgagccg ccacccgcgc cgcccgctgc gacggagccg
     7261 cccgccgcgc cggagccgcc ggcgcccgag ccccccactg cggcggagcc gccgcccgcg
     7321 cccgccg
//
LOCUS       DASVPL010000120        27104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_327721, whole genome shotgun sequence.
ACCESSION   DASVPL010000120 DASVPL010000000
VERSION     DASVPL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 27104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27104
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327721"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VF517_06005"
     CDS             complement(<1..841)
                     /locus_tag="VF517_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8052528.1"
                     /translation="MPAPVLVVGAIVSTQVGASLAKGLFDDLGAGGTTFLRVGFAALV
                     LVALSRPALAGHARADLWTAAAFGLSLAGMNLCFYEAIDRIPLGIAVTIEFLGPLGVA
                     VALSRRRLDFAWVVLAGSGIALFANRGEAGSLDPVGVALVLAAACFWGAYILMSARTG
                     RAFPGGTGLSLAMLVGAALLVPVGVADAGGALLEPELLAAGAGVAVLASAIPYTLELE
                     ALRRLPAGVFGIFMSIEPAIAALAGWLVLSQGLGARDVVAIALVVAASAGASLTAPPA
                     AEAP"
     gene            complement(886..2484)
                     /locus_tag="VF517_06010"
     CDS             complement(886..2484)
                     /locus_tag="VF517_06010"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8052529.1"
                     /translation="MDIGIGSVFAGHRLEGVAGRGGMGVVYKATHLALDRTVALKLIA
                     PEISGDESFRERFKQESMTAAALDHPNVVPIYDAGEEHGQLYVTMRHVPGTDLRALIE
                     QSGGLPPAEAASIIAQIADALDAAHERGLVHRDVKPGNILIEDRGGKRHAYLTDFGLT
                     KHAATDSGMTKTGMFVGTLDYIAPEQLQGQAVDARTDVYSLTCVLYQAVTGQVPYPRD
                     SEPSKMWAHMGEDPPSVNQARADVPPAFEEVIKRGMAKKPEDRYPSTGDLGRATLAAA
                     RDQQATQIERSVATGDAAPGHAMPPPVVSMPAPPTGVTSSPAPTAGPRAPAAPPPPPG
                     YSPPPPPPPTSGPATGPPQWQPTAAGGPGPGPGGPKNRTPLIIGGALAGLAVLVLVLA
                     LALGGGGDEKKDDPGTTSAQTDGTDTSGSTDTSGDAGTGGDAAEGDIRLAVETTLGAV
                     ATDKSDVFCGGLSVRYQNKQFGGPTECEAAFKKNDLPAQFTPEEIRIDTVDVQGQDAT
                     VTLSGGEVIRLVKGQNFWEIDGLG"
     gene            complement(2662..2916)
                     /gene="rpmA"
                     /locus_tag="VF517_06015"
     CDS             complement(2662..2916)
                     /gene="rpmA"
                     /locus_tag="VF517_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX8052530.1"
                     /translation="MAHKKGLGSSRNGRDSNSKRLGVKVFAGEAVTGGEIIVRQRGTV
                     FKPGDGVGIGRDHSIFATRPGTVEFTSGWRGRVISVTPVE"
     gene            complement(2906..3262)
                     /gene="rplU"
                     /locus_tag="VF517_06020"
     CDS             complement(2906..3262)
                     /gene="rplU"
                     /locus_tag="VF517_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX8052531.1"
                     /translation="MYAIVKAGGKQYRVEEGQSLLVDRMREDEGAKVNLEALMLADGD
                     NTVLDPAEIEKIKVEAVVVGHERGKKVHVLKFKPKRGYKRRSGHRSDLTRLEIREIKL
                     LSRRPAKKKEDDEDGS"
     gene            3355..3513
                     /locus_tag="VF517_06025"
     CDS             3355..3513
                     /locus_tag="VF517_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052532.1"
                     /translation="MGKAIRCECGEMVKGEDEQAVLAAAHEHIEAKHPDMVTTINDSQ
                     LRAMMEDD"
     gene            3506..4852
                     /locus_tag="VF517_06030"
     CDS             3506..4852
                     /locus_tag="VF517_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8052533.1"
                     /translation="MTRDAVLPPGPRVPVPLQTVAWIVRAPEVMDACARRYGGAFTMR
                     LPRLDPLVFVSDPEDVKAVFTGSAELLHAGEANELLEPILGSESILLLDEQRHLRQRK
                     LMLPAFHGDRLKRYRDQMRAVTEAELARWPRGLSEPARPRMQDITLEIIMRTVFGLEE
                     GERLARLRSALKGLLEWTTARRRLFVAIVAGPKRVSQSDVFGLTKARAAADELIFDEI
                     RRRSEEGGLAEREDVLSMLLQARDEEGRPMTPVELRDELMTLLVAGHETTATALAWAV
                     EQLARHPEALARLEGGEEEYLDAVVQETLRLRPVIVVVVRRLTEAMELGGYSLPAGSN
                     VAPCIYLMHRRPEIYPDPEAFRPERFLETPPGTYTWLPFGGGIRRCLGAQFAQMEMKV
                     VLSTLLENARLRPTTARPERAIRRAITFVPDRGGEIVLDEAPAGRLAAAAHPSARA"
     gene            complement(4903..6819)
                     /locus_tag="VF517_06035"
     CDS             complement(4903..6819)
                     /locus_tag="VF517_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX8052534.1"
                     /translation="MKKKVLVTVDRGETRVAILEAEGQPSGSAEQAKGRRGKGGGGGD
                     GDWRVGELYIERRWSRSIVGNVYKGKVDNVLPGLEAAFVDIGIDKNGFLHVDDIVMPG
                     VEVQRRGRVSGKGQRIAQLLKPGQEIIVQVVKDPLKTKGARLSMQLSIAGRYLVHVPQ
                     GEGIGVSRRLEDKERERLRKQTKGLDLGGGGAIIRTAARGAKREDFEREIKYLHKLHE
                     LLQQRAAAASAPEMVFQEADLSVRVVRDIFSEQFESALVDDSKQYERLISFFTRTAPE
                     LLDRVEHYKDEEPLFERFGVDKVVQSTLSRRVDLPSGGYLIIDYAEAMTVIDVNSGSF
                     TGRGKGARLEDTISKTNLEAAEETVRQLRLRDIGGIIVIDFIDMARARNRDAVLKTLR
                     KMLDEDRTKTYVMEISPLGLVEMTRQNVTDGVREILTKTCPTCEGEGVVLSEETVAID
                     VERKLRELVAEKPTPQAFLIQVHPQVSSFLINGPGRPILDLERELDKHFHFEGSEALA
                     IDHFAVMMEGTHEEVEERALPFREGEEVLVTIAEPHMYEENDAVAKLDGYVISVEDAM
                     RHIGQKRLVRIEHVSRSAATASLVEGQEPPPEDDGGGPGGSDGSGGGDGEEPRRRRGR
                     RGGRGRRKATAASD"
     gene            complement(6964..8160)
                     /gene="rodA"
                     /locus_tag="VF517_06040"
     CDS             complement(6964..8160)
                     /gene="rodA"
                     /locus_tag="VF517_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX8052535.1"
                     /translation="MSAALGEQVQRFRDAERQRSPLLRIDPLLFLAAVGLIACSIYVV
                     GKATHDDIPGDPNYYEMRQGIYAAVGIALMLLVARFDYSRLRELKLGIYGAMIGLILL
                     VFALGAAARGSQRWIELPFFRLQPSELGKVLLIVALAGFTVDRMRRLGDRDTTSRLLL
                     LAILPSILVVAQPDLGSGLVYMAIATAILFVGGTKWTHFAALGGLAAVAIAAVLVVGP
                     AIGVEVLKDYQKDRLTAFVNPTDDPAEQGYQLQQSTVAIGSGEKTGRGPEGATQTKLD
                     FLPEHHTDFIFSVVGEMFGFAGAAFVLSLYALLIWRALRILTMSKNLYGTLIVGGVVA
                     MLMFQVFVNVGMTVGLGPITGVPLPLMSYGGSSVMVTFLAIGLLQSVHAQARLTSDAK
                     RRELVV"
     gene            complement(8167..10044)
                     /gene="mrdA"
                     /locus_tag="VF517_06045"
     CDS             complement(8167..10044)
                     /gene="mrdA"
                     /locus_tag="VF517_06045"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8052536.1"
                     /translation="MYLDRDTRTPITPQLAVRVAIIGGVALVLFAVVFFRLWYLQVLS
                     GDQYRAEANNNRVREITVQAPRGRIVDRNGRVLVDNRIAFAVTLSPDKLPKDRHGKKK
                     LYSRLADVLGMKQREVDREVSEQLKLLPFSPATVKSDVEPEEYSYLLENADRFPGVQV
                     EKVALRKYPFETVGAHLFGTVGEVTEEQLDDKRYRGVNQGARVGQSGIEYSYDRYLRG
                     RDGANRVQVDALGNFRSQLREAPPEAGRQLRLAIDYDVQRVGQQALGANKAAFVVMDV
                     KTGEVRALGSSPSFDPNIFAKRIRQSDFERLNSKELGEPLVNRATQSVYPTGSTFKLV
                     TATAALEGGLITPGSVIYDGGSLRVGGVTFKNAGGAVNGPVALRKAIQVSSDVFFYNL
                     GREANSAGDGHLIQDWAKRLGMGRDPGIDIPAASSGLIPSPEWRNKLFKKKQTDRPWT
                     VGDNINLSVGQGDLQVAPLQLAIAYAAIANGGYIVRPHLGERVEDADGRVIQEFNQPS
                     RRKLDIKPQNRQAILEGLRAAASAPGGTSTGVFKDFPIPVAGKTGTAEKGAGRADQSW
                     YAALAPYPNPRYVVITTFEAGGFGAETAAPAARRILAELFGVKNQGPKTQSQGGGPVY
                     E"
     gene            complement(10069..10644)
                     /gene="mreD"
                     /locus_tag="VF517_06050"
     CDS             complement(10069..10644)
                     /gene="mreD"
                     /locus_tag="VF517_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX8052537.1"
                     /translation="MTVSVGSVVRVGLLLLVAVVTQISGFSGLRIFGGVFDLIPLVVA
                     AIALYGGSVTGAAVGFALGLLADLALGHNLGASSLVLTAVGYGVGRFGELRDPAHGLI
                     AIPVGGAATFGYVVAFGLVNFMLEIDASISALVLRDALVTVVLNAWLALPVFGIVKRV
                     LRPVLASEPGHRRRPAETRPTGPLGLRGLEV"
     gene            complement(10641..11504)
                     /gene="mreC"
                     /locus_tag="VF517_06055"
     CDS             complement(10641..11504)
                     /gene="mreC"
                     /locus_tag="VF517_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX8052538.1"
                     /translation="MYDRKVIRRRRAALALFVALSVVLLSAYFGEGPGGMLHGVQKGA
                     QEVLSPIESGASKVFKPFRDLFNWVGDAFDANSENKKLKKDLAQARTELAQARAATRE
                     NEQLRAMVDLQKEPGFPQGLNPVTGRVIARSPTDWSSTIQINKGTDDGVQRDDAVITG
                     DGLVGKIAQASGGTATVQLITDGESNVSAMAVPEGTSGVVRTPVGDPDDLQLDFVRQV
                     KNVKKGQTVVTSGFRSGRLQSLFPRGIPIGSVREVDRDELETYQRVHVDPFADFRRID
                     IVQVLTGGSEE"
     gene            complement(11558..12604)
                     /locus_tag="VF517_06060"
     CDS             complement(11558..12604)
                     /locus_tag="VF517_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8052539.1"
                     /translation="MGLFSYLTGMGGRDMAVDLGTANTLVYVRGRGIVLSEPSVVAID
                     SRTGEVHAVGIEAKRMLGRTPGTIQAIRPLKDGVIADFDVTEQMLRHFIQKVHQNRWA
                     HPRVVVCVPSGVTGVEKRAVEEACLSAGARQAYLIEEPMAAAIGAGLPVGEPTGNMVV
                     DIGGGTSEVAVISLGGIVVSQSIRIGGDELDEAIINYVKREYKLMIGQQTAEEVKLEI
                     GSAFPLGEEVQAEIRGRDLVSGLPKTVVLTSEEVRHALEEPLQAIVDAVKETLDRTPP
                     ELAADIMDRGIMMAGGGSLLQGLDERLRDETQMPAHLAESPLTCVAVGSGRSLEEFEA
                     IHRANRTNSRTRRR"
     gene            12821..13756
                     /locus_tag="VF517_06065"
     CDS             12821..13756
                     /locus_tag="VF517_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX8052540.1"
                     /translation="MSDQRLQSSPWALVRAQHGVVSTAQLLALGFTPSWVKARVAAGR
                     LHRLFRGVYAVGRPDVTREGWWMAAVLACGEGSALSHGNAAAHYGILAEPKGPIHVSV
                     PLTARRSYAGIRPHRRTLRAQDVTKHERIPVTTVATTIADLAASMRRGPLEGAINQAD
                     ILGLITVPALRAAVDEMPPRPGTRPLRETIDRRTFRFTRSQLERAFIPLALSAGLPRP
                     ETCVVVNGWEVDFYWPGLDFVVEADSLTYHRTAAQQHRDRLRDQAHASAGTERLRFTH
                     SQIKYEPGYVRALLTTVARRLLREVGRELQQLGHR"
     gene            complement(13709..14254)
                     /locus_tag="VF517_06070"
     CDS             complement(13709..14254)
                     /locus_tag="VF517_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX8052541.1"
                     /translation="MAAPLTLASRSPQRRAILTQLGVEFVVVEPDVEEVGEGDPRWLV
                     VENALRKARSVVGERVLGVDTDVVLDGWVFGKPASVDAARDDLLALSARVHEVFSGIA
                     LKEGGSERTAVSVTRVRFRRLDASLLDWYLRSGEWEGRAGGYAIQGRGAALVESIEGD
                     FWNVVGLPVPELLKLAPDLAQ"
     gene            complement(14264..14671)
                     /gene="ndk"
                     /locus_tag="VF517_06075"
     CDS             complement(14264..14671)
                     /gene="ndk"
                     /locus_tag="VF517_06075"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX8052542.1"
                     /translation="MDRTLILVKPDAFARALTGEVIARFESKGLRIVALKHMTVDRDT
                     AERHYEEHRERPFFGDLVDFITGGPLVAMVLEGHEAVKAARQLIGATNPIEAAPGSIR
                     GDFGLEVQTNLVHGSDSPESAQREVGIFFPELS"
     gene            complement(14715..15548)
                     /locus_tag="VF517_06080"
     CDS             complement(14715..15548)
                     /locus_tag="VF517_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX8052543.1"
                     /translation="MVAPAIFGITNDGLNLAVKLLLLFLVAIWLALVFWTYADARRRV
                     GDPMLVTCAAAAAFFFPFVGSIVYAIVRPPEYLEDARERELEIASAEARLLQVQESTC
                     PHCGHDIEKTFLRCPHCTRRLKEPCTNCARPLDPRWQMCPYCEAEVVQQAGGGGQRRR
                     GRRPGAGAAEAGAAPAERAAPARQTAAAREPARETVREAPRARPARESRGPAPETRAD
                     PARREAPTQPARVLRDSEPRGNSRGADGSARPQAPDPSTRVGDTAEQPTRRPRPSADP
                     A"
     gene            complement(15718..17031)
                     /locus_tag="VF517_06085"
     CDS             complement(15718..17031)
                     /locus_tag="VF517_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX8052544.1"
                     /translation="MSGERPMSLREAEEYLLDLELFGMRFGLDRMHRLMTALGMPQRR
                     FASIHVVGTNGKSSTTRMIAAILQRHGVATGTYTSPHLRTFAERIEVGERPLSGEEFG
                     AAVAEVVRAARMVDRTADEDDRVTQFEALTAAAYHELARRRVEVAVIEAGLGGRYDAT
                     NVIPSRVQVLTGVGLEHTRWLGPTIKDIAEEKLAVVRDHATLVIPAELDPAAEEVVAR
                     VAAERHARVVRAEGAARAAAPGPGSPPPAASPAPAASPPALPGFQRGNFALARAAAEA
                     FLGRLDAAAVARAAAEVRVPGRLQVVADHPLTILDGAHNPSGMRALAAALPEVAGDRR
                     PRVGVVSVLDDKDATGMLAELAPVLDRVVFTRSANPRSLSPATLESHWGQLGGPPAET
                     VPDPRTALARAREIAGPGGAAVATGSIYLIADLVREDAAGRASAL"
     gene            complement(17054..17521)
                     /locus_tag="VF517_06090"
     CDS             complement(17054..17521)
                     /locus_tag="VF517_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052545.1"
                     /translation="MGAAAADGQDIAQGPRLSTRIGATGLVVLMSIGGFAMWIATPVA
                     WLWLGSQLTESRRPDGTLYVIVAAGILITMSVLGLLLGRMNRIHGTITGRLTETTARN
                     VRVAWSRPQSASQEGRTPVNALEVIMVVCVLIAGVLFFVWFFLFAGSSLDYQT"
     gene            complement(17582..18256)
                     /locus_tag="VF517_06095"
     CDS             complement(17582..18256)
                     /locus_tag="VF517_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8052546.1"
                     /translation="MKQLADEVYQLKYFPPNAINVYLLGETLIDAATRRDGKRILRQI
                     EGRKVSQHALTHAHADHQGASKYVCERLGIPFLVGEADVEGAEGGAEVIRSKQPPSRW
                     NLLSTKAFGGPGHKVDQVLREGDDIGAGFTVLDTPGHSAGHVAFWRESDRMLILGDVL
                     FNQHPLLGIPGLRQPPDFFTPDVPRNRDSARRLAELEPALVCFGHGPPLRDTGKFVEF
                     VRGLPG"
     gene            complement(18391..19992)
                     /locus_tag="VF517_06100"
     CDS             complement(18391..19992)
                     /locus_tag="VF517_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8052547.1"
                     /translation="MSQEQLQRYEDVIARFLRGEYLSLDAAGHVNSWTRAAEERFGWS
                     ASETTGEELAEAIAVPEAQEEARALVDPVVRGEAGEESAGAQFVLASHRRDGSRLDTE
                     VAIVPIRMGDGYHLNLALQDITTHRGNPVELHRMKKRHADVLRLIVIALDGGELPDPL
                     GDDGWKPGGERVEARWQPAGALVIFDGTEVEVVAEPEAAGAAGGGAAGVAASGPPASS
                     SEVDWLRAENERLRRELRAAQDETERVRSTAPSAARPADDLDDPSITPDSIAEALRDD
                     GFALYGQPVLDLRSDEVAQHELLLRMIGPDGGMVLPQAFFGTARRAGMTAAIDQWVVR
                     RAVRTIAEQAQVGRDIKLEVNLSVESLHDPALLPTIERELASTSIDPARLILEVPERV
                     AIEDSDGASRLAKRLRAMGCSFALDDFGTSFGSFRFLKDLPIDFLKIDGDLIVTLSES
                     RTAQLVVKALVDLAKGTGAETIAVFASDDEALRILREIGVGYAQGHKVGRPRPIAEAL
                     SAVEAAPLRAVDDAQAATGTANAAN"
     gene            complement(20203..21480)
                     /gene="clpX"
                     /locus_tag="VF517_06105"
     CDS             complement(20203..21480)
                     /gene="clpX"
                     /locus_tag="VF517_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX8052548.1"
                     /translation="MARPTDSNEQLLCSFCGKSQRQVKKLIAGPGVYICDECIDLCNE
                     IIDEELTGPATFDLENLPKPKEINSVLQEYVVGQEPAKRTLSVAVYNHYKRVQMMQSD
                     EQDGLELQKSNILLLGPTGCGKTLLAQTLAKILNVPFAIADATALTEAGYVGEDVENI
                     LLKLIQAADFDVKKAETGIIYIDEVDKIARKADNPSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFLTIDTTNILFICGGAFADLDKVVERRIGHKGVGFGAAVRSKS
                     NKDTGELFEQVLPEDLVDYGLIPEFIGRLPVISVIHQLSRDDLVSILTEPRNALVKQF
                     QRFFEFDGIDLVFADESLGAIANKALERETGARGLRSILEETLLDVQFELPSRRDVKK
                     CVVTKETIEKGLNPTLVTEAAPEEDTGAAAESA"
     gene            complement(21600..21923)
                     /locus_tag="VF517_06110"
     CDS             complement(21600..21923)
                     /locus_tag="VF517_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8052549.1"
                     /translation="MQTQAFSVSHHRLGDIRVVVPRREIDCVTAPALSAALDQAMEEG
                     SSKVVVDLCDVTFMDSSGAHVLLEAADRLASEGRPFSVICVRGAISRLLEILNLTDRF
                     ALHAE"
     gene            complement(22060..23463)
                     /gene="tig"
                     /locus_tag="VF517_06115"
     CDS             complement(22060..23463)
                     /gene="tig"
                     /locus_tag="VF517_06115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX8052550.1"
                     /translation="MPAQLTTRTSELEESRVRVDVEVAPDDLERELQSAAQALGSDLK
                     IPGFRKGKVPPQVVLQRVGREAVLDEAVRRALPGWYERAVADAGIAVVGDPSVDLETL
                     PGKGDPLQFSIEVGVRPKATLGDYKGLEVGRRDPEVSDEQVDAELQQIRESGASLETV
                     ERAAATGDFVVLDFTGTIDGEPFEGGEARGYPLELGSGRLIEGFEEQLVGASAGDERQ
                     VEVTFPADYRAEELAGKDVVFAVTVKEVKEKRLPELDDDMAIEAGYDTLAELREEVAG
                     RIREAQEREIEGEFREAAIDAAAATATIELPHALVHAKAHEMWGNMRRRLQQQGIDAS
                     QYLQITGKSEHDLIDESEPDAERALRRESVLAAVIEAEGIEVSEDELLDSLRQAMASG
                     PSGADEDTLQKTLERIKEDGRDDALREDVAMRKAVDLLVEQAKPIPLDKAEAREKLWT
                     PEKEQKEKATQIWTPGT"
     gene            23489..23626
                     /locus_tag="VF517_06120"
     CDS             23489..23626
                     /locus_tag="VF517_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052551.1"
                     /translation="MPPQRRLVTWIYTGPAGHLWSAVADIVSAWVRWGWSDVRPRYSK
                     R"
     gene            complement(23611..24342)
                     /locus_tag="VF517_06125"
     CDS             complement(23611..24342)
                     /locus_tag="VF517_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HEX8052552.1"
                     /translation="MRVGVITGTGTYELDSAVAGAVEVVHVSRHEPGHPRLSNHVDHR
                     GNIQRLREARVDAIVAVTVCGAVDPTVPLGSLIVFDDLHFLANRLPDGSLCTLHDTPG
                     DPGRGHWIFESPFAEGVRQALLAGSREAGVPARDGGCYGHVDGPRFNTRAEIRSLALA
                     GVTAVSQTAGPETVLAGEAEIPYALLGYATDYANGVTADPTPVEELVRLIGASGATFR
                     SVLAEALPLLARADLAPTGTVYRFE"
     gene            complement(24373..24747)
                     /locus_tag="VF517_06130"
     CDS             complement(24373..24747)
                     /locus_tag="VF517_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052553.1"
                     /translation="MRRPIACRIRLDGMPVRSETILTEAGPNALVLSTTIRDRGIWLD
                     STYLGHGSADTQITHLLVAPGRCGETEARTVPHDDVPVIHVRRLCLYDHFQRLQDFLE
                     GLGHVGQIHGLDHAIEAVEQLS"
     gene            24832..25095
                     /locus_tag="VF517_06135"
     CDS             24832..25095
                     /locus_tag="VF517_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX8052554.1"
                     /translation="MIWPVPIYDFHCEACGTTFEELVRGDSAPPCPSCGAAEPRRLIS
                     QVSPPARVGLRGAAAKRSDSLRKAREERKREQRAARRESDRGG"
     gene            complement(25092..25244)
                     /locus_tag="VF517_06140"
     CDS             complement(25092..25244)
                     /locus_tag="VF517_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052555.1"
                     /translation="MLNLSAAAPECTNCGEPLKPERRRPGRRPTARRFSPGTVAERRD
                     VVPPPA"
     gene            25377..26339
                     /locus_tag="VF517_06145"
     CDS             25377..26339
                     /locus_tag="VF517_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052556.1"
                     /translation="MRRKLPIGGSLAAVAAIASALASAPAASGSEYSESLARNVEREI
                     VYVDPKATPRVSTTEAGAIRLQITRKDIGRIKVAVVPERRAEREGGASGLAAAVARDL
                     ELRGTLLVVAGSSIHAITSHPASDSTVAAVREAFERHEGDRGDQIRAAVDGIAAVDPG
                     PSADAGGSTGGGSSGGLPGGDGDDFFDEINDAVKFTTFIVGIAIALPFIAVFLWILLR
                     FRRARKEQDEDWDFAQEQLRAELIELGDEIRALDVDTSMPGANALGLADYEAAIAQYD
                     RANLALDRAEETPRLRVAEARAALKEGKRRMSDAKVRLGVTPIP"
     gene            26404..>27104
                     /locus_tag="VF517_06150"
     CDS             26404..>27104
                     /locus_tag="VF517_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052557.1"
                     /translation="MPPPGGPAYPRRLDADLVERVVAAASRGSRAPRFPEIEGAKPKL
                     SGPEPKVEGLSRSGQAALKSAGFRTPESLAGLALGDLGADATKLSLSDLDALHRERIA
                     RAWDDTKLSKKAKRSLIEHRIDPVNFPGFLKKCGGTIGPIRPGIDLPQDFDLDDFLVP
                     ADPWGPLGPVGPGGGGGPGPRPPGPEPDPVPWHELRDLAVQPGELAKAVDLLAGGQVD
                     PGRVEEERVRTLLAGP"
BASE COUNT         3696 a         9651 c         9825 g         3932 t
ORIGIN      
        1 cgggcgcctc ggccgccggc ggggcggtga gcgatgcccc cgccgaggcg gccacgacca
       61 gcgcgatggc taccacgtcg cgcgccccga gcccctggct cagcaccagc cagccggcca
      121 gcgcggcgat cgccggctcg atgctcatga agatcccgaa cacgcccgcg gggagtcgcc
      181 gcagggcctc gagctcgagc gtgtacggga tcgccgaggc gagcaccgcc acgccggcgc
      241 ccgccgcgag cagctccggc tcgagcagcg cgccgccggc gtcggcgacc ccgaccggca
      301 cgagcagcgc cgcaccgacc agcatcgcca gcgataggcc cgtgcctccc gggaacgcac
      361 gcccggtccg cgcgctcatg aggatgtagg ccccccagaa gcacgcggcc gcgagcacca
      421 gcgccacccc gacgggatcg agcgagccgg cctcaccgcg gttcgcgaac agcgcgattc
      481 cggagcccgc gagaaccacc caggcgaagt cgagccgccg ccgcgacagc gccaccgcca
      541 cgccgagcgg cccgaggaac tcgatcgtca ccgcgatgcc cagcgggatc cggtcgatcg
      601 cctcgtagaa gcacaggttc atgccggcga ggctgagccc gaaggcggcg gcggtccaca
      661 ggtcggcgcg cgcgtggccc gcgagcgccg gacgcgacag cgccaccagc acgagcgcgg
      721 cgaacccgac gcgcaggaag gtcgtgccgc ccgctcccag gtcgtcgaac aggcccttgg
      781 caagcgaggc accgacctgc gtggagacga tggcgcccac cacgagtacg ggcgcgggca
      841 acggcgtgag acccgcgatc gcgggcgcgg gagacggcga ccggctcagc cgagcccgtc
      901 gatttcccag aagttctggc ccttgacgag cctgatgacc tcgccgcccg acagggtgac
      961 ggtggcatcc tggccctgca cgtcgaccgt gtcgatgcgg atctcctccg gggtgaactg
     1021 ggcgggcagg tcgttcttct tgaacgccgc ctcgcactcg gtcggtccgc cgaactgctt
     1081 gttctggtag cgcaccgaga gcccgccgca gaacacgtcg gacttgtcgg tcgccaccgc
     1141 gccgagggtg gtctcgaccg ccagcctgat gtcgccctcg gccgcgtcgc ccccggtgcc
     1201 ggcgtcaccc gacgtgtcgg tgctgccgct cgtgtcggtg ccgtcggtct gtgccgaggt
     1261 cgtgcccgga tcgtccttct tctcgtcgcc gccgccgccg agcgccagcg cgaggaccag
     1321 gacgagcacg gccaggccgg ccagcgcgcc gccgatgatg agcggggtgc ggttcttcgg
     1381 gccgccggga ccggggccgg ggccgcccgc ggccgtgggc tgccactgcg gcgggccggt
     1441 cgcggggccc gaggtgggcg gcggcggggg cggaggcgag tagccgggcg gcgggggcgg
     1501 cgcggccggg gcgcgcgggc cggccgtggg cgccggcgag ctggtgaccc ccgtgggcgg
     1561 cgcgggcatc gagaccaccg gcgggggcat cgcgtggccc ggcgcggcgt cgccggtcgc
     1621 gacactgcgc tcgatctgcg tggcctgctg gtccctggcg gcggcgaggg tggcccggcc
     1681 gaggtcgccg gtggacgggt agcggtcctc gggcttcttg gccatcccgc gcttgatgac
     1741 ctcctcgaac gcgggcggca cgtcggcacg cgcctggttg accgagggcg ggtcctcgcc
     1801 catgtgcgcc cacatcttcg agggctcgct gtcgcgcggg tagggcacct gcccggtgac
     1861 cgcctggtag agcacacacg tgagcgagta gacgtcggtc cgagcgtcga ccgcctggcc
     1921 ctggagctgc tcgggcgcga tgtagtcgag cgtgccgacg aacatcccgg tcttggtcat
     1981 cccgctgtcg gtcgcggcgt gcttggtgag gccgaagtcg gtgaggtacg cgtggcgctt
     2041 gccgccgcgg tcctcgatca ggatgttgcc cggcttcacg tcgcggtgga cgagtccgcg
     2101 ctcgtgcgcg gcgtccagcg cgtcggcgat ctgggcgatg atgctcgccg cctcggccgg
     2161 cggcagcccg ccgctctgct cgatgagcgc acgcaggtcg gtgccgggga cgtgccgcat
     2221 cgtcacgtag agctggccgt gctcctcgcc ggcgtcgtag atcgggacca cgttcgggtg
     2281 gtcgagcgcg gcggccgtca tcgactcctg cttgaagcgc tcgcggaagc tctcgtcgcc
     2341 cgagatctcc ggagcgatca gcttcagcgc gacggtgcgg tcgagcgcga ggtgcgtggc
     2401 cttgtagacc acgcccatcc cgccgcggcc ggcaacgccc tcgaggcggt gtccggcgaa
     2461 gaccgatccg atgccgatat ccatcggccg gaagctagat gatcggcgcc gggtccgcgt
     2521 gcccgtgggg ccgcgaaaac cgagcgggac aaggacgcag ggtgatgtcc atgcatgcgt
     2581 gcatggacga acccgaggac gccgtcaccg cgaaggtttg cagcggtatc cacggatgcg
     2641 tgggtccggg gccggtcatc tctactcgac aggagtgacg gatatgacgc ggccgcgcca
     2701 gccgctcgtg aactcgaccg tccccggccg ggttgcgaag atgctgtggt cgcggccgat
     2761 gccgacgccg tcgcccggct tgaagacggt cccgcgctgg cgcacgatga tctcgccgcc
     2821 ggtcacggcc tcgccggcga agaccttcac cccgagacgc ttgctgttgg agtcgcgccc
     2881 gttgcggctg gagccgaggc ccttcttatg agccatcctc gtcgtcctcc ttcttcttgg
     2941 cggggcggcg ggagagcagc ttgatctcgc ggatctcgag acgggtgagg tccgagcggt
     3001 gcccgctgcg gcgcttgtag ccgcgcttgg gcttgaactt caggacgtgg accttcttcc
     3061 cgcgctcgtg cccgacgacg acggcctcga ccttgatctt ctcgatctcc gccgggtcga
     3121 ggacggtgtt gtcgccgtcg gcgagcatca gcgcctcgag gttgaccttc gcgccctcgt
     3181 cctcgcgcat gcggtccacg agcagggact ggccctcctc cacgcggtat tgcttcccgc
     3241 ctgctttgac gatcgcgtac atgacaaaga gatggcgccc tccggtgggc gccgagcggg
     3301 tcagtctagc ggcgatccgg gggtacgatc aggcccccga cggaggaggc ccgcatgggc
     3361 aaggcgatcc gctgcgagtg cggagagatg gtcaagggcg aggatgagca ggcggtcctg
     3421 gccgccgctc acgagcacat cgaggcgaag catccggaca tggtcacgac catcaacgac
     3481 tcccagctgc gggcgatgat ggaggatgac tagggacgcc gtgctcccgc cggggccgcg
     3541 ggtcccggtg cccctccaga ccgtggcctg gatcgtccgt gcgccggagg tgatggacgc
     3601 ctgcgcccgc cgctacgggg gcgcgttcac catgcggctg ccgcgcctcg acccgctcgt
     3661 gttcgtgagc gatcccgagg acgtaaaggc cgtgttcacc ggcagcgccg agctgctgca
     3721 cgcgggtgag gcgaacgagt tgctcgagcc gatactcggc tcggagtcga tcctcctgct
     3781 cgacgagcag cgccacctgc gccagcgcaa gctgatgctc cccgcgttcc acggcgaccg
     3841 gctcaagcgc taccgggacc agatgcgcgc ggtgaccgag gccgagctcg cgcgctggcc
     3901 gcgcggcctg agcgagcccg cccggccgcg gatgcaggac atcacgctcg agatcatcat
     3961 gcggacggtc ttcgggctcg aggagggcga gcggctggcg cggctgcgga gcgccctgaa
     4021 ggggctgctc gagtggacca cggcgcggcg gcggctgttc gtggcgatcg tcgccggacc
     4081 gaagcgggtc tcgcagtcgg acgtattcgg tctcacgaag gcgcgcgcgg ccgccgacga
     4141 gctgatcttc gacgagatcc gccgccgctc ggaggagggc ggcctcgccg agcgcgagga
     4201 cgtgctctcg atgctgctcc aggcgcgcga cgaggagggc cggccgatga ccccggtcga
     4261 gctgcgcgac gagctgatga cgctgctcgt cgccggccac gagaccaccg caaccgcgct
     4321 ggcgtgggcc gtcgagcagc tcgcgcgcca ccccgaggcg ctggcgcggc tcgagggcgg
     4381 cgaggaggag tacctcgacg cggtggtgca ggagaccctg cggctgcgac ccgtgatcgt
     4441 cgtggtggtg cggcggctga cggaggcgat ggagctgggc gggtactcgc tgcccgcggg
     4501 ctcgaacgtc gcgccgtgca tctacctgat gcaccgccgg ccggagatct atcccgaccc
     4561 ggaggcgttc cgcccggaac gcttcctcga gaccccgcca gggacctaca cctggctccc
     4621 gttcggcggc gggatccgcc gctgcctggg cgcgcagttc gcgcagatgg agatgaaggt
     4681 ggtgctctcg acgctgctcg agaacgcgcg cctgcgaccc acgacggcgc gtcccgagcg
     4741 cgcgatacgc cgcgcgatca cattcgtgcc ggaccgcggc ggcgagatcg tgctcgacga
     4801 ggcgccggcc ggccggctcg ccgcggcggc gcatccgtcc gcccgcgcct agtccgacgc
     4861 gcgcggcgtc tccgcccgcc cgcgcgtagt ccgacgcgag ccttagtccg aggccgccgt
     4921 ggccttccgc cggccgcgcc cgccgcgccg cccgcgccgg cgccgcggct cctcgccgtc
     4981 gccgccgccc gagccgtccg acccgccggg cccgccgccg tcgtcctccg gcgggggttc
     5041 ctggccctcg accagcgacg ccgtcgctgc cgagcgcgac acgtgctcga tccgcaccag
     5101 ccgcttctga ccgatgtggc gcatggcgtc ctcgaccgag atgacgtagc cgtcgagctt
     5161 cgccaccgcg tcgttctcct cgtacatgtg cggctcggcg atcgtgacga gcacctcctc
     5221 gccctcgcgg aacggcagcg cgcgctcctc gacctcctcg tgcgtcccct ccatcatcac
     5281 cgcaaagtgg tctatcgcga gggcctccga gccctcgaag tggaagtgct tgtccagctc
     5341 gcgctcgagg tcgaggatcg gccggcccgg cccgttgatc aggaacgacg aaacctgcgg
     5401 gtgcacctgg atcaggaacg cctgcggggt cggcttctcc gcgacgagct cgcgcagctt
     5461 gcgctccacg tcgatcgcga cggtctcctc ggagagcacc acgccctcgc cttcacaggt
     5521 cgggcacgtc ttggtgagga tttcgcgcac gccgtcggtc acgttctggc gcgtcatctc
     5581 gacgagcccg agcggcgaga tctccatcac gtaggtcttc gtgcggtcct cgtcgagcat
     5641 cttgcgcagc gtcttcagca ccgcgtcgcg gttgcgcgcg cgggccatgt cgatgaagtc
     5701 gatgacgatg atcccgccga tgtcgcgcag cctgagctgg cggacggtct cctcggcggc
     5761 ctcgaggttg gtcttcgaga tcgtgtcctc gagccgcgcg cccttgccgc ggccggtgaa
     5821 cgaccccgag ttgacgtcga tgaccgtcat ggcctcggcg tagtcgatga tcaggtagcc
     5881 gcccgacggg aggtcgaccc gccggctgag cgtggactgg acgaccttgt ccaccccgaa
     5941 gcgctcgaac agcggctcct cgtccttgta gtgctcgaca cggtcgagca gctcgggcgc
     6001 ggtgcgcgtg aagaacgaga tgaggcgctc gtactgcttg ctgtcgtcca cgagcgcgct
     6061 ctcgaactgc tcggagaaga tgtcgcgcac cacgcgcacg gacaggtcgg cctcctggaa
     6121 gaccatctcc ggcgcactgg ccgccgccgc gcgctgctgg agaagctcgt gcagcttgtg
     6181 gaggtacttg atctcgcgct cgaagtcctc gcgcttggcg ccgcgcgcgg ccgtgcggat
     6241 gatcgcgccg ccgccgccga ggtcgagccc cttggtctgc ttgcgcaggc gctcgcgctc
     6301 cttgtcctcg agccggcgcg agacgccgat gccctcgccc tgcggcacgt gcacgaggta
     6361 gcgcccggcg atcgagagct gcatcgagag gcgcgccccc ttcgtcttga gggggtcctt
     6421 caccacctgc acgatgatct cctggccggg cttcaggagc tgcgcgatgc gctggccctt
     6481 gccgctcacg cggccgcggc gctgcacctc gacgccgggc atcacgatgt cgtcgacgtg
     6541 caggaagccg ttcttgtcga tgccgatgtc gacgaacgcc gcctcgaggc ccgggaggac
     6601 gttgtccacc ttgcccttgt agacgttgcc cacgatcgac cggctccagc gccgctcgat
     6661 gtacagctcg cctacgcgcc agtcgccgtc gccgcccccg ccgcccttgc cgcggcggcc
     6721 cttcgcctgc tcagcggatc cggacggctg tccctccgcc tcgaggatcg caactcgcgt
     6781 ttcgccacgg tccaccgtga cgagcacctt cttcttcaac gaaactcctt aggttcatcg
     6841 cctgcctgcc cgtacgcggc gtgaccgcgg cgcgcgcgga aaaggaggcg cgcgtcggag
     6901 ggcgtgaggc gagggaaaag ctcacgagcc ctgaggttag acggtggggc ggcgggtcct
     6961 gcgtcagacg acgagctcgc ggcgcttcgc gtcgctcgtg agacgcgcct gcgcgtgcac
     7021 ggattgcagc agcccgatcg ccaggaacgt gaccatcacc gacgatccgc cgtagctcat
     7081 cagcggcagc ggcacccccg tgatcggacc gaggccgacg gtcatcccga cgttcacgaa
     7141 cacctggaac atcagcatcg ccacgacgcc cccgacgatc agcgttccgt acaggttctt
     7201 cgacatcgtg agtatccgca gcgctcgcca tatgagcagc gcgtagagcg acagcacgaa
     7261 cgccgcgccc gcgaagccga acatctcgcc gaccacgctg aagatgaagt ccgtgtggtg
     7321 ctcggggagg aagtcgagct tggtctgcgt cgcgccctcc gggccgcggc cggtcttctc
     7381 gcccgacccg atggcgaccg tggactgctg cagctggtag ccctgctcgg ccgggtcgtc
     7441 ggtcggattg acgaacgccg tcagccggtc cttctggtag tccttgagca cctcgacgcc
     7501 gatcgccggg ccgaccacca gcacggccgc gatggccacc gccgcgagcc cgccgagcgc
     7561 cgcgaagtgc gtccacttgg tgccgccgac gaacaggatc gcggtggcga tcgccatgta
     7621 gacgaggccg gagccgaggt cgggctgcgc gaccacgagt atcgacggga ggatcgcgag
     7681 caggagcagg cggctggtgg tgtcgcggtc gcccagccgc cgcatccggt ccacggtgaa
     7741 cccggccagc gccacgatca gcagcacctt gccgagctcc gacggctgga ggcggaagaa
     7801 cggcagctcg atccagcgct gcgacccgcg cgcggcggcg ccgagcgcga acaccagcag
     7861 gatcagcccg atcattgctc cgtagatgcc gagcttgagc tcgcgcaggc gcgagtagtc
     7921 gaagcgcgcg acgagcagca tcagggcgat ccccaccgcg gcgtagatcc cctggcgcat
     7981 ctcgtagtag ttcgggtcgc cggggatgtc gtcgtgggtg gccttgccga cgacgtagat
     8041 gctgcacgcg atcaggccga ccgccgcgag gaacagcagc gggtcgatcc gcagcagcgg
     8101 cgagcgctgg cgctccgcgt cgcggaagcg ctggacctgc tcgccgaggg ccgcgctcac
     8161 aggggcctac tcatagacgg ggccgccacc ctgggactgc gtcttcggcc cctggttctt
     8221 cacgccgaac agctcggcga ggatgcgccg cgcggcgggg gccgcggtct cggcgccgaa
     8281 gccgccggcc tcgaacgtgg tgatcacgac gtagcgcggg ttcgggtacg gagcgagcgc
     8341 ggcgtaccag gactggtcgg cgcggcccgc tcccttctcc gccgtgccgg tcttgccggc
     8401 cacggggatc gggaagtcct tgaacacgcc ggtcgaggtg ccgcccggcg cgctcgcggc
     8461 ggcgcgcagg ccctcgagga tcgcctggcg gttctggggc ttgatgtcga gcttgcggcg
     8521 cgagggctgg ttgaactcct ggatcacacg cccgtccgcg tcctcgacgc gctcgccgag
     8581 gtgtgggcgc acgatgtagc caccgttggc gatcgccgcg taggcgatcg cgagctggag
     8641 cggcgccacc tggaggtcgc cctggcccac cgacaggttg atgttgtcgc ccaccgtcca
     8701 gggccggtcg gtctgcttct tcttgaagag cttgttgcgc cactcggggc tggggatcag
     8761 cccggagctg gcggcgggga tgtcgatgcc ggggtcgcgc cccatgccga gccgcttcgc
     8821 ccagtcctgg atcaggtggc cgtcgccggc gctattggcc tcgcggccga ggttgtagaa
     8881 gaagacgtcg ctcgagacct ggatcgcctt gcgcagggcg accgggccgt tcacggcgcc
     8941 gcccgcgttc ttgaacgtca cgccgccgac gcgcagcgag ccgccgtcgt agatcacgct
     9001 gcccggcgtg atcaggccgc cctcgagcgc ggccgtggcg gtgaccagct tgaacgtcga
     9061 tccggtggga tagaccgact gggtggcgcg gttcacgagc ggctcgccga gctccttgct
     9121 gttcaggcgc tcgaagtccg actggcggat ccgcttggcg aagatgttcg ggtcgaacga
     9181 cggcgagctg ccgagcgcgc gtacctcgcc ggtcttcacg tccatgacca cgaaggcggc
     9241 cttgttcgcc ccgagcgcct gctggccgac ccgctgcacg tcgtagtcga tcgcgagccg
     9301 cagctggcgg cccgcctcgg gcggcgcctc gcggagctgg gagcggaagt tgccgagggc
     9361 gtcgacctgc acgcggttgg cgccgtcgcg gccgcgcagg tagcggtcgt aggagtactc
     9421 gatgccggac tggcccacgc gcgcgccctg gttcacgccg cggtagcgct tgtcgtcgag
     9481 ctgctcctcg gtcacctcgc cgaccgtgcc gaacaggtgg gcgccgacgg tctcgaacgg
     9541 gtacttgcgc agcgcgacct tctcgacctg cacgccgggg aagcggtcgg cgttctcgag
     9601 caggtaggag tactcctcgg gctcgacgtc ggacttcacg gtggcgggtg agaacggcag
     9661 cagcttgagc tgctcgctca cctcgcggtc gacctcccgc tgcttcatcc cgagcacgtc
     9721 ggcgagccgc gagtagagct tcttcttccc gtggcggtcc ttcggcagct tgtccgggct
     9781 cagcgtgacc gcgaaggcga tccggttgtc caccagcacg cggccgttgc gatcgacgat
     9841 ccgcccgcgc ggcgcctgca cggtgatctc gcgcacgcgg ttgttgttcg cctccgcgcg
     9901 gtactggtcg ccgctcagca cctggaggta ccagaggcgg aagaagacga ccgcgaacag
     9961 cacgagcgcg acgccgccga tgatggcgac gcgcaccgcg agctgcggcg tgatcggcgt
    10021 gcgtgtgtcg cggtctagat acatggggcg ggagcctccg ggcgcactct agacctcgag
    10081 cccgcggagg ccgagcggcc cggtcgggcg ggtctcggcg ggccggcgcc ggtggccggg
    10141 ctcgctcgcg aggacggggc gcagcacgcg cttgacgatg ccgaacaccg gcagcgcgag
    10201 ccaggcgttc agcaccacgg tgacgagtgc gtcacgcagc acgagggcgc tgatcgaggc
    10261 gtctatctcc agcatgaagt tgacgaggcc gaaggcgacg acgtagccga acgtggcggc
    10321 gccgccgacc gggatcgcga tcaggccgtg cgccgggtcg cgcagctcgc cgaagcgccc
    10381 gacgccgtag ccgaccgcgg tgagcaccag cgacgaggcg ccgaggttgt ggccgagcgc
    10441 gaggtcggcg agcaggccga gcgcgaagcc gacggccgcg ccggtcacgc tgccgccgta
    10501 gagggcgatc gcggcgacca cgagcgggat caggtcgaag acgccgccga agatgcgcag
    10561 cccggagaag ccggagatct gggtcacgac cgccaccagc aggagcaggc cgacgcggac
    10621 cacggagccg acgctgacgg tcattcctcg gagcccccgg tcaggacctg gacgatgtcg
    10681 atccggcgga agtccgcgaa cgggtcgacg tgcacgcgct ggtaggtctc gagctcgtcg
    10741 cggtcgacct cgcgcacgct gccgatcggg atcccgcgcg ggaagagcga ctggaggcgg
    10801 ccggagcgga agccggaggt cacgacggtc tggcccttct tgacgttctt cacctggcgg
    10861 acgaagtcga gctggaggtc gtccgggtcc ccgaccgggg tgcggaccac cccgctcgtg
    10921 ccctcgggca ccgccatggc gctcacgttg ctctcgccgt cggtgatgag ctgcacggtg
    10981 gccgtgccgc cggacgcctg cgcgatcttc ccgaccaggc cgtcgccggt gatgaccgca
    11041 tcgtcgcgct ggaccccgtc gtcggttccc ttgttgatct ggatcgtgga ggaccagtcg
    11101 gtcggcgagc gcgcgatcac gcgcccggtc actgggttga gcccctgcgg gaagccgggt
    11161 tccttctgga ggtcgaccat cgcgcggagc tgctcgttct cgcgcgtggc ggcgcgggcc
    11221 tgggccagct ccgtgcgcgc ctgggcgagg tccttcttca gcttcttgtt ctcgctgttg
    11281 gcgtcgaagg cgtcgccgac ccagttgaag aggtcgcgga acggcttgaa caccttgctc
    11341 gcgcccgact cgatcgggga cagcacctcc tgggcgccct tctggacgcc gtggagcatg
    11401 ccgccggggc cctcgccgaa gtaggcgctg agcaggacga cggacagggc gacgaacagc
    11461 gcaagcgccg cccggcgcct gcggatcacc ttcctgtcgt acacctctgg ctcccctccc
    11521 tcgcgcggat cgtctcagcg cggtgggtca tgctcagtta ccttcgtcgg gtccgtgaat
    11581 tggtccggtt cgcgcggtgg atcgcctcga actcctcgag cgagcggccg gagccgaccg
    11641 ccacgcaggt cagcggcgac tcggcgaggt gcgcgggcat ctgcgtctcg tcgcgcaggc
    11701 gctcgtcgag tccctggagc agcgagccgc cgccggccat catgatgccg cggtccatga
    11761 tgtcggccgc cagctcgggc ggggtgcggt cgagcgtctc cttgaccgcg tccacgatcg
    11821 cctggagcgg ctcctcgagc gcgtgccgga cctcctcgct cgtgagcacc accgtcttcg
    11881 gcaggcccga cacgaggtcg cgcccgcgga tctcggcctg gacctcctcg ccgagcggga
    11941 aggcgctgcc gatctcgagc ttcacctcct cggccgtctg ctggccgatc atcagcttgt
    12001 actcgcgctt gacgtagttg atgatcgcct cgtcgagctc gtcgccgccg atgcggatcg
    12061 actgcgagac gacgatgccg ccgaggctga tcaccgccac ctcggacgtg ccgccgccga
    12121 tgtccacgac catgttgccg gtcggctcgc cgacggggag gccggcgccg atcgcggccg
    12181 ccatcggctc ctcgatcagg taggcctggc gggcgccggc ggagaggcag gcttcctcga
    12241 ccgcgcgctt ctcgacgccg gtgacgccgg agggcacgca gacgaccacg cgcgggtggg
    12301 cccagcggtt ctggtggacc ttctggatga agtggcggag catctgctcg gtcacgtcga
    12361 agtcggcgat cacgccgtcc ttcagcggcc ggatggcctg gatcgtgccc ggcgtgcggc
    12421 cgagcatccg cttggcctcg atgcccaccg cgtggacctc gccggtgcgg gagtcgatcg
    12481 cgaccactga cggctcgctg agcacgatgc cgcgcccgcg cacgtagacg agcgtgttgg
    12541 cggtgccgag atcgacggcc atgtcgcggc cgcccattcc agttaggtag gagaaaaggc
    12601 ccatgtcagg aagcagttat tggcacgagg agcagccagg ctccggccga ccccgcgcga
    12661 actctcggcc cctcgaggag ggccggtccg ggagcagagg agtgtaacga acgcttttgt
    12721 gctgattgca gggcgcaaag ccgcctgcaa cggggcgcgg agagcggccg tcgatggggt
    12781 cccgggggcg aattctcacc gattggagcc ttaggtcggt atgagcgacc aaaggctcca
    12841 atcgtctcca tgggcactcg tccgcgcgca gcacggggtg gtttcgacag cccagttgct
    12901 tgcgctcggg ttcacgccgt cgtgggtcaa ggctcgggtg gccgcgggac gcctccatcg
    12961 cctgttccgc ggcgtgtacg cggtcgggcg gcccgatgtc acacgcgagg ggtggtggat
    13021 ggcggccgtg ctcgcctgcg gggagggatc cgcgctgagc cacggcaacg cggcggcgca
    13081 ctacgggatc ctcgcggagc cgaaggggcc gatccacgtc tcggtccccc tgactgcccg
    13141 ccgctcctac gccggcatcc gcccccaccg ccgcacgctg cgcgcgcagg acgtgacgaa
    13201 gcacgaacga atcccggtga ccaccgtggc caccaccatc gccgacctcg ccgcgagcat
    13261 gcggcgcggg ccgctcgagg gcgcgatcaa ccaggccgac atcctcgggc tgatcaccgt
    13321 gccggccctg cgagcggcgg tggacgagat gccgccgcgg ccgggcacgc ggcccctccg
    13381 cgagacgatc gaccgccgca cgttccgctt cacgcgctcg cagctggagc gggcgttcat
    13441 cccgcttgcc ctgagtgccg gtcttccccg tcccgagacg tgcgtcgtcg tgaacgggtg
    13501 ggaggtcgat ttctactggc cggggctcga cttcgtcgta gaggccgaca gcctcaccta
    13561 ccaccgtacg gcggcccagc agcacaggga ccggctgcgc gatcaggcgc acgcctcggc
    13621 cgggaccgag cggctgcgct tcacccactc ccagatcaag tacgagcccg gctacgtgcg
    13681 agcactgctg acgacggtcg cgcggcggct actgcgcgag gtcgggcgcg agcttcagca
    13741 gctcgggcac cggtaggccg acgacgttcc agaagtcgcc ctcgatcgac tcgaccagcg
    13801 ccgccccgcg gccctggatc gcgtagccgc cggcgcgacc ctcccactcg ccggagcgca
    13861 ggtaccagtc gagcagcgaa gcatcgagcc gtcggaaccg gacccgagtg acgctgacgg
    13921 cggtcctttc agagcccccc tctttaagtg ctattccgga aaagacctca tgcacgcgcg
    13981 cggagagcgc gagcaggtcg tcacgggcgg catcgacgga ggcgggcttc ccgaacaccc
    14041 acccgtcgag caccacgtcg gtatcgacgc cgagcacccg ctcgcccacc accgagcgcg
    14101 ccttgcgcag cgcgttctcg acgacgagcc agcgcgggtc cccctcccca acctcctcga
    14161 cgtccggctc gaccaccacg aactcgaccc ctagctgggt caggatcgcg cgccgctgag
    14221 gcgagcggga tgcgagggtg agcggcgcgg ccaccgcggc cgcttaggac agctcgggga
    14281 agaagatccc gacctcgcgc tgggccgact cgggggagtc cgagccgtgc acgaggttgg
    14341 tctggacctc gaggccgaag tcgccgcgga tcgatccggg ggcggcctcg atcgggttgg
    14401 tcgccccaat gagctggcgg gcggccttca cggcctcgtg gccctcgagg accatcgcga
    14461 ccagcgggcc gccggtgatg aagtcgacga ggtcgccgaa gaagggccgc tcgcgatgct
    14521 cctcgtagtg gcgctcggcg gtgtcgcggt cgacggtcat gtgcttgagc gcgacgatgc
    14581 ggagcccctt gctctcgaac cgcgcgatga cctcacccgt gagggcgcgc gcgaatgcgt
    14641 cgggcttgac gaggatgagc gtccggtcca tgagcgcggg cagcttagcg ggggccgggc
    14701 gggacgagcc gccgctacgc cgggtcggcg ctcgggcggg ggcggcgggt cggctgctcg
    14761 gccgtgtccc ccacgcgcgt gctcgggtcc ggcgcctgcg ggcgggcgga gccgtccgcg
    14821 ccgcgtgaat tgccgcgcgg ctctgagtcg cgcagcacgc gggcgggctg ggtcggggcc
    14881 tcgcgccggg cgggatccgc gcgggtctcc ggggcggggc cgcggctctc gcgggccggg
    14941 cgtgcacgcg gggcctcgcg gacggtctcc cgcgcgggct cgcgagcggc agcggtctgg
    15001 cgcgcgggcg cggcgcgctc ggctggggcc gcgccggcct cggccgcccc tgcgccgggc
    15061 ctgcgcccac ggcggcgctg acctccgcca ccggcctgct gcaccacctc ggcctcgcaa
    15121 tacgggcaca tctgccagcg cgggtcgagc ggccgcgcgc agttcgtgca cggctccttc
    15181 aaccgccgcg tgcagtgcgg gcagcgcagg aaggtcttct cgatgtcgtg accgcagtgc
    15241 gggcaggtcg actcctggac ctggagcagt cgcgcctcgg cggaggcgat ctcgagctcg
    15301 cgctcgcgcg cgtcctccag gtactcgggc gggcgcacga tcgcgtagac gatcgagccg
    15361 acgaacggga agaagaaggc cgccgcggcc gcgcaggtca cgagcatcgg atcgccgacc
    15421 cgccgccggg cgtcggcgta ggtccagaag acgagcgcta gccagatcgc gacaaggaac
    15481 agcagcagca gcttgaccgc aaggttcagc ccgtcattgg tgatcccgaa gatggcgggg
    15541 gcgaccactg cgcggggagt gtagccgcgc cgcagacggc ctcacccccg ccggtgagac
    15601 cccgtcagag cagcatgcgg ccgagcgcgt acccgatccc gaagaacacg aggatcaccg
    15661 ccgcgaccag gacggccacc aggccgacca tcgcgaggat gctgggaccg tcgtcgctca
    15721 tagtgcggag gcccgcccgg cggcgtcctc gcggacgagg tcggcgatca ggtagatcga
    15781 gcccgtcgcg acggcggcgc cgccgggccc ggctatctcg cgggcgcggg cgagggcggt
    15841 gcgggggtcc ggcacggtct cggccggtgg gccgccgagc tggccccagt gcgactcgag
    15901 cgtggccggg gagagcgatc gcggattcgc cgagcgggtg aagaccacgc ggtcgagcac
    15961 gggcgcgagt tcggccagca tcccggtggc gtccttgtcg tcgagcacgc tgacgacgcc
    16021 cacccgcggg cgccggtcgc cggcgacctc gggcagcgcg gccgcgagcg cgcgcatccc
    16081 cgacgggttg tgcgcgccgt cgaggatcgt gagcgggtga tcggccacga cctggaggcg
    16141 acccgggacc cgcacctcgg ccgccgcccg cgcgacggcc gccgcgtcga gccggccgag
    16201 gaatgcctcg gcggcggcgc gcgcgagggc gaagttgccg cgctggaagc cggggagggc
    16261 gggcggcgaa gccgcggggg cgggcgaagc cgcggggggc ggcgagcccg gccccggcgc
    16321 ggccgcgcga gctgccccct ccgcccgcac gacccgcgcg tggcgctccg ccgccacgcg
    16381 cgcgaccacc tcctccgccg cggggtcgag ctcggcgggg atcaccagag tcgcgtggtc
    16441 gcgcaccacc gcgagcttct cctccgcgat gtccttgatc gtcggcccga gccagcgggt
    16501 gtgctcgagc ccgacgccgg tcagcacctg cacgcgcgat gggatcacgt tcgtggcgtc
    16561 gtagcgcccg ccgagccccg cctcgatcac cgccacctcc actcgcctgc gcgcgagctc
    16621 gtggtacgcc gcggcggtga gcgcctcgaa ctgagtgacg cgatcgtcct cgtcggccgt
    16681 gcgatccacc atccgcgccg cgcgcaccac ctccgccacg gccgccccga actcctcgcc
    16741 gctcagcggc cgctccccca cctcgatccg ctccgcgaac gtccgcaggt gcggggaggt
    16801 gtaggtgccg gtggccacgc cgtggcgctg gaggatcgcg gcgatcatcc gggtcgtcga
    16861 cgacttcccg ttcgtcccca ccacgtgtat cgaggcgaag cgccgctgcg gcatcccgag
    16921 cgccgtcatc agcctgtgca tgcggtcgag cccgaaccgc atgccgaaga gctcgaggtc
    16981 gagcaggtac tcctcggcct cgcgcaggct catcggccgc tcgccgctca cggcgatgag
    17041 cgtaccggcg cgcctaggtc tggtagtcga ggctcgagcc ggcgaagagg aagaaccaga
    17101 cgaagaacag gacgcccgcg atcaggacgc agacgaccat gatcacctcg agcgcgttca
    17161 ccggcgtccg cccctcctgc gacgccgact gcgggcgcga ccaggcaacc cgcacgttgc
    17221 gggcggtcgt ctccgtgagc cgccccgtga tcgtgccgtg gatccggttc atgcgaccga
    17281 gcagcaggcc cagcaccgac atcgtgatca ggatcccggc ggcgacgatc acgtagagcg
    17341 tgccgtccgg gcgccgcgac tccgtgagct gcgagccgag ccacagccat gccaccggcg
    17401 tcgcgatcca catcgcgaac ccgccgatcg acatcagcac caccaggccc gtggcgccga
    17461 tgcgcgtgga gagccgtggc ccctgcgcga tgtcctgccc gtcggccgcc gccgccccca
    17521 tgctctctat ctcccccaga acggccgcac ggaatccgga aatcccgttc agcgctcagc
    17581 gctagcccgg gagcccgcgt acgaactcga cgaacttccc cgtgtcccgc agtggcggcc
    17641 cgtggccgaa gcagaccaga gcgggctcga gctcggcgag ccgccgcgcc gagtcgcggt
    17701 tgcgcgggac gtcgggcgtg aagaagtccg gcggttgccg cagcccggga atccccagca
    17761 gcggatgctg gttgaacagg acgtcaccga ggatcagcat gcggtcggac tcgcgccaga
    17821 acgccacgtg cccggcgctg tggccgggcg tgtcgagcac ggtgaacccg gcgccgatgt
    17881 cgtcgccctc gcgcagcact tggtcgacct tgtgtccggg gccgccgaac gccttcgtgc
    17941 tcagcaggtt ccagcgactg gggggctgct tgctgcggat gacctccgcc ccgccctcgg
    18001 cgccctccac gtccgcctcg cccacgagga acgggattcc cagccgctcg cagacgtact
    18061 tgctcgcccc ctggtggtcc gcatgggcgt gcgtcagcgc gtgctggctg accttgcgcc
    18121 cctcgatctg gcgcaggatc cgcttgccgt cgcggcgagt ggcggcgtcg atcagcgtct
    18181 cgcccaggag gtagacgttg atcgcgttcg gcgggaagta cttgagctgg tagacctcgt
    18241 cggcgagttg cttcatggcg cgtcagccta aagccacggc cgcgtcgggc gcgcggaggg
    18301 acgtcgggcg tgcggaggga cggcgggcgc gcggagggac ggcgggcgcg ggcggccgac
    18361 gcccggcgag cgcgggcggg cgaggcccgg ttagttcgcc gcgttcgccg tgccggtcgc
    18421 cgcctgcgcg tcgtcgaccg cgcgcagcgg cgccgcctcg accgcggaca gcgcctcggc
    18481 gatcggccgt gggcgcccga ccttgtggcc ctgcgcgtac cccaccccga tctcgcgcag
    18541 tatccgcagc gcctcgtcgt cggaggcgaa caccgcgatc gtctcggcgc cggtcccctt
    18601 cgcgaggtcc acgagcgcct tcaccacgag ctgcgccgtc cggctctccg acagcgtcac
    18661 gatcaggtcg ccgtcgatct tcaggaagtc gatcggcagg tccttgagga agcggaacga
    18721 gccgaagctc gtgccgaagt cgtcgagcgc gaaggagcag cccatcgcgc gcagccgctt
    18781 ggccaaccgc gaggcgccgt cggagtcctc gatcgccacg cgctccggca cctcgaggat
    18841 cagcctggcg gggtcgatcg aggtgctcgc gagctcgcgc tcgatggtgg gcagcagcgc
    18901 ggggtcgtgg agcgactcca cggacaggtt cacctcgagc ttgatgtcgc gcccgacctg
    18961 cgcctgctcg gcgatcgtgc ggaccgcgcg ccgcacgacc cactggtcga tcgccgcggt
    19021 catccccgcc cgccgcgccg tgccgaagaa cgcctgcggc agcaccatgc cgccgtcggg
    19081 cccgatcatc cgcagcagca gctcgtgctg agcgacctcg tcggagcgca ggtcgaggac
    19141 cggctgaccg tacagcgcga agccgtcgtc gcgcagggcc tcggcgatgg agtccggggt
    19201 gatcgaggga tcgtcgaggt cgtcggcggg ccgcgccgcg ctgggggcgg tggagcgcac
    19261 cctctccgtc tcgtcctggg cggcgcgcag ctcccgccgc agccgctcgt tctccgcgcg
    19321 cagccagtcg acctcggagg acgaggcggg cgggccggag gcggcgacac ccgcggcccc
    19381 gccgccggcg gcccccgcgg cctccggctc ggcgaccacc tcgacctcgg tcccgtcgaa
    19441 gatcacgagc gcgccggcgg gctgccagcg cgcctcaacc cgctcgcccc cgggcttcca
    19501 gccgtcgtcg ccgagcgggt cgggaagctc gccgccgtcg agcgcgatca cgatcagccg
    19561 caggacgtcg gcgtggcgct tcttcatccg gtgcagctcg accgggttgc cgcgatgggt
    19621 ggtgatgtcc tggagcgcga ggttcaggtg gtagccgtcg cccatccgga tcgggacgat
    19681 cgcgacctcg gtgtcgagcc gcgagccgtc gcggcgatgg ctcgcgagca cgaactgcgc
    19741 gccggccgac tcctcgcccg cctcgccgcg cacgacggga tccaccagcg cacgcgcctc
    19801 ctcctgcgcc tccggcaccg cgatcgcctc ggccagctcc tcgccggtcg tctcgctggc
    19861 ggaccacccg aagcgctcct cggccgcccg ggtccacgag ttcacgtgac cggcggcgtc
    19921 gagggacagg tactcgcccc gcaggaagcg cgcgatgacg tcctcgtagc gctgcagctg
    19981 ctcctggctc acgccaaggg cggcctctgc gaggtcggcg ggctcctgga tttgctcgtt
    20041 ggggatgtcg gacactggag gggcgagtcg cgctggcggg gaggcgcggt ctctacaccg
    20101 tcattcggca ccctgcgcgc gtatatgccc ccctttgccg agggggtcga acaaccgtcc
    20161 agcggcgccg tgcaacggcg cttgcagggg aggctgcgcc tactaggcgg actcggccgc
    20221 ggcgcccgtg tcctcctcgg gcgccgcctc ggtgaccagg gtggggttga gccccttctc
    20281 gatcgtctcc ttggtcacca cgcacttctt cacatcgcgc ctcgagggca gctcgaactg
    20341 cacgtcgagg agcgtctcct cgaggatcga acgcagcccg cgggcacccg tctcccgctc
    20401 gagcgccttg ttggcgatgg ccccgagcga ctcgtcggcg aagacgaggt cgatgccgtc
    20461 gaactcgaag aagcgctgga actgcttgac gagcgcgttg cgcggctcgg tgaggatcga
    20521 gacgaggtcg tcgcgcgaga gctggtggat caccgagatg accgggaggc gcccgatgaa
    20581 ctccgggatc aggccgtagt ccacgaggtc ctcgggcagc acctgctcga acagctctcc
    20641 cgtgtccttg ttcgacttcg agcgcaccgc ggcgccgaac ccgacgccct tgtggccgat
    20701 ccgccgctcg accaccttgt cgaggtccgc gaacgcaccg ccgcagatga acaggatgtt
    20761 cgtggtgtcg atcgtgagga actcctggtg cgggtgcttg cgcccgccct gcggcggcac
    20821 cgacgccacc gtgccctcga ggatcttgag gagcgcctgc tggacgccct cgccggacac
    20881 gtcgcgcgtg atcgacgggt tgtcggcctt gcgcgcgatc ttgtcgacct cgtcgatgta
    20941 gatgatcccg gtctcggcct tcttgacgtc gaagtcggcg gcctggatca gcttcaggag
    21001 gatgttctcg acgtcctcgc cgacgtagcc ggcctcggtc agcgccgtgg cgtcggcgat
    21061 cgcgaacggc acgttgagga tcttggcgag cgtctgcgcg agcagcgtct tgccgcagcc
    21121 ggttgggccg agcagcagga tgttcgactt ctggagctcg agcccgtcct gctcgtcgga
    21181 ctgcatcatc tggacgcgct tgtagtggtt gtagacggcc accgacagcg tgcgcttggc
    21241 cggctcctgc cccaccacgt actcctggag gaccgagttg atctccttgg gcttcggcag
    21301 gttctccaga tcgaacgtcg ccgggccggt cagctcctcg tcgatgatct cgttacagag
    21361 atcgatgcac tcgtcgcaga tgtagacgcc ggggcccgcg atcagcttct tgacctgccg
    21421 ctgagacttg ccgcagaagc tgcagaggag ctgttcgttt gaatccgtgg gccgtgccaa
    21481 ggctctcccc ccctaaccgc ggatgcggta gcgggcctca aaaggtagcg cggcggcggt
    21541 catcgttgcg cttgttttcg ccaggtcggc cggattcctt caccccggag gggcgctgcc
    21601 tactccgcgt gcagggcgaa gcggtccgtg aggttcagga tctcgagcag ccgcgagatg
    21661 gccccgcgga cgcagatgac cgagaacggg cgcccctcgg aggcgagccg gtcggccgcc
    21721 tcgagcagca cgtgcgcgcc gctcgagtcc atgaacgtca cgtcgcagag gtcgacgacg
    21781 accttgctcg acccctcctc catcgcctgg tcgagcgcgg ccgacagggc cggcgcggtg
    21841 acgcagtcga tctcccgccg cggcacgacg acgcggatgt cgccgaggcg atggtgggat
    21901 acggagaagg cctgggtctg catggggctc ctagctgcgc ccgggaccgt ctgagcgagt
    21961 gcctgccttg gcaagcgacg gtctccggag ggcaaggccg accctaacat cgcccccgcc
    22021 cccgcgccag tgcccgattt ggggggttct ggaccccttt tacgtgccgg gcgtccagat
    22081 ctgcgtggcc ttctccttct gctccttctc gggcgtccag agcttctcgc gcgcctcggc
    22141 cttgtcgagc gggatcggct tcgcctgctc gaccaggagg tccaccgcct tgcgcatcgc
    22201 gacgtcctcg cgcagggcgt cgtcgcggcc gtcctccttg atccgctcga gcgtcttctg
    22261 aagggtgtcc tcgtcagccc cgctcggccc ggaggccatc gcctggcgca gcgagtcgag
    22321 cagctcgtcc tcgctgacct cgatgccctc ggcctcgatc acagcggcca gcacgctctc
    22381 gcggcgcagc gcgcgctcgg cgtccggctc ggactcgtcg atcaggtcgt gctcgctctt
    22441 gccggtgatc tggaggtact gcgacgcgtc gatcccctgc tgctggagcc ggcggcgcat
    22501 gttgccccac atctcgtgtg ccttcgcgtg gaccagcgcg tgcgggagct cgatcgtggc
    22561 ggtcgccgcg gcggcgtcga tggcggcctc gcggaactcg ccctcgatct cgcgctcctg
    22621 cgcctcgcgg atgcgaccgg ccacctcctc gcgcagctcg gccagcgtgt cgtagccggc
    22681 ctcgatcgcc atgtcgtcgt cgagctccgg gaggcgcttc tccttgacct ccttgacggt
    22741 gaccgcgaag accacgtcct tgccggcgag ctcctcggcg cggtagtcgg ccgggaacgt
    22801 gacctcgacc tggcgctcgt cgccggcgga ggcgcccacg agctgctcct cgaagccctc
    22861 gatgagccgg cccgagccga gctcgagcgg atagccgcgg gcctcgccgc cctcgaaggg
    22921 ctcgccgtcg atagttcccg tgaagtcgag caccacgaag tcaccggtcg cggccgcgcg
    22981 ctcgacggtc tcgagcgagg cgccggactc gcggatctgc tggagctcgg cgtcgacctg
    23041 ctcgtcgctg acctcgggat cgcggcggcc cacctcgagg cccttgtagt cgccgagcgt
    23101 cgccttcggc cgcacgccga cctcgatcga gaactggagc gggtcgccct tgccggggag
    23161 ggtctcgagg tcgacgctcg ggtcgcccac gaccgcgatg cccgcgtcgg ccacggcgcg
    23221 ctcgtaccag ccgggcaggg cgcggcggac cgcctcgtcg agcacggcct cgcggccgac
    23281 ccgctggagg accacctgcg gaggcacctt gcccttgcgg aagccgggaa tcttcaggtc
    23341 gctgccgaga gcctgcgccg cgctctggag ctcgcgctcg aggtcgtcgg gggcgacctc
    23401 gacgtctacc cggacgcgcg actcctcgag ctcgctcgtg cgcgtggtga gctgggcggg
    23461 catggccgcg ccacgatagc gtggcggcgt gccacctcag cggcgtctcg tgacctggat
    23521 ctacaccggt ccggccggcc acctctggtc ggccgtcgcg gacatcgtgt cggcgtgggt
    23581 gcgctggggc tggagcgacg tgcggccgcg ttactcgaag cggtagaccg tcccggtggg
    23641 cgcgaggtcc gcccgcgcga gcagggggag cgcctccgcg agcaccgacc ggaaggtcgc
    23701 gccgctggcg ccgatcaggc ggaccagctc ctccaccggg gtcgggtcgg ccgtcacgcc
    23761 gttggcgtag tcggtggcgt agccgagcag cgcgtacggg atctcggcct cgccggcgag
    23821 cacggtctcc ggccccgcgg tctggctcac ggccgtgacc ccggccagcg ccagcgaacg
    23881 gatctccgcg cgcgtgttga agcgcgggcc gtctacgtgg ccgtagcagc cgccgtcgcg
    23941 cgcgggcacg cccgcctcgc gcgagccggc cagcagcgcc tggcggacac cctccgcgaa
    24001 cgggctctcg aagatccagt ggccgcggcc cggatcgccc ggggtgtcgt ggagcgtgca
    24061 cagcgacccg tcggggaggc ggttggcgag gaagtggagg tcgtcgaaga cgatcagcga
    24121 gccgagcggg accgtcgggt ccaccgcccc gcagaccgtg acggcgacga tggcgtcgac
    24181 gcgcgcctcg cgcaggcgct ggatgttgcc gcggtggtcg acgtggttcg agagccgcgg
    24241 gtggcccggc tcgtgacgcg agacgtgcac gacctcgacg gcgccggcca cggcggagtc
    24301 gagctcgtag gtccccgtac cggtgatgac gccgacccgc atcgcgggcg gcatgctacg
    24361 tcagcggtgc ggctaggaca gctgctcgac ggcctcgatc gcgtgatcga ggccgtggat
    24421 ctggccgacg tggccgagcc cttccaggaa gtcctggagg cgctggaagt ggtcgtagag
    24481 gcagagccgg cgcacgtgga tgaccggcac gtcgtcgtgc gggaccgtac gggcctcggt
    24541 ctcgccgcag cgcccggggg ccaccaggag gtgggtgatc tgcgtgtcgg cgctgccgtg
    24601 gccgaggtag gtgctgtcga gccagatgcc gcggtcgcgg atcgtcgtcg agagcaccag
    24661 cgcgttcggg ccggcctcgg tgaggatggt ctcgctgcgt acgggcatcc catcgaggcg
    24721 gatccggcag gcgatcgggc ggcgcatacg agagcaacgg taggccagct catgtgccgg
    24781 cgcaagcttc gcgtgagtca ttgcacgggt tcctggacgg atgtacgagt catgatctgg
    24841 ccggtgccga tctacgactt ccactgcgag gcgtgcggga cgaccttcga ggagctggtg
    24901 cgcggcgact cggcgccgcc gtgcccgagc tgcggcgcgg ccgagccacg gcggctgatc
    24961 tcgcaggtct cgccgccggc gcgggtcggg ctgcgcggcg cagccgcgaa gcgcagcgac
    25021 tcgctccgca aggcacgcga ggagcgaaag cgcgagcagc gcgcggcgcg gcgggagagc
    25081 gaccggggcg gctaggcggg cggcgggacg acgtcgcggc gctcggccac ggtccccggc
    25141 gagaaccggc gcgccgtcgg gcgccggccg ggacggcgcc gctcgggctt gagcggctcg
    25201 ccgcagttgg tgcactcggg ggcggcggcg ctcaggttca gcacgtccca gcggatcgaa
    25261 ccgcacccat cgcaacgaag caggccaccc atcgtgggct ggcggtaccc ggtgggtagg
    25321 gcttgaaaca tggcgcacaa gcgcggtgtg cctttgcgca ggtagcatcg cccgaagtgc
    25381 gccgcaagtt gccgatcgga gggtccctcg ccgcagttgc ggcgatcgcg tccgcgctcg
    25441 cctcggcgcc cgccgcgagc ggatccgagt actccgagag cctcgcgcgc aacgtcgagc
    25501 gcgagatcgt ctacgtggat ccgaaggcga ccccgcgggt gtccacgacg gaggccggcg
    25561 cgatccggct ccagatcacg cgcaaggaca tcggtcgcat caaggtcgcg gtggtgcccg
    25621 agcgccgcgc cgagcgcgag ggcggcgcct ccggcctcgc cgcggccgtg gcgcgcgacc
    25681 tcgaactgcg tgggacgctg ctcgtggtgg ccgggtcgag catccacgcg atcacctcgc
    25741 acccggccag cgactcgacg gtcgccgccg ttcgcgaggc gttcgagcgc cacgagggcg
    25801 accgcggcga ccagatccgc gccgccgtgg acggcatcgc ggccgtcgac cccgggccca
    25861 gcgcggacgc ggggggctcc acgggcggcg gctcctcggg cgggcttccc ggcggggacg
    25921 gtgacgactt cttcgacgag atcaacgacg ccgtgaagtt cacgacgttc atcgtgggga
    25981 tcgcgatcgc gctcccgttc atcgccgtct tcctctggat cctgctgcgc ttccggcgcg
    26041 cgcgcaagga gcaggacgag gactgggact tcgcgcagga gcagctccgc gccgagctga
    26101 tcgagctcgg tgacgagatt cgcgcgctcg acgtggacac ctcgatgccc ggcgccaacg
    26161 cgctcggcct ggccgactac gaggcggcca tcgcgcagta cgaccgcgcg aacctcgccc
    26221 tcgatcgagc cgaggagacc ccgcggctgc gagttgcgga ggcacgcgcg gcactgaagg
    26281 agggcaagcg ccggatgtcg gacgcgaagg tgcgtctggg tgtcacgccc atcccgtagg
    26341 gggacgacgc cggccggcac gaggaattcc caccgttcgc actcgggcgg ttagggtcgg
    26401 ccgatgccac ctcccggtgg acccgcgtac ccccgccgcc tcgacgccga cctggtcgag
    26461 cgcgtggtcg ccgccgcctc gcgcgggagc cgcgcgccgc gctttccgga gatcgagggc
    26521 gccaagccga agctgagcgg cccggagccg aaggtcgagg gactgtcgcg cagcggccag
    26581 gcggcgctga agtccgccgg cttccgcacc ccggagtcac tcgccggcct cgcgctcggc
    26641 gacctgggcg ccgacgccac gaagctctcg ctgtccgatc tcgacgcgct ccaccgcgag
    26701 cggatcgcgc gcgcgtggga cgacacgaag ctgtcgaaga aggccaagcg ctcgctgatc
    26761 gagcaccgga tcgacccggt caacttcccc ggcttcctga agaagtgcgg cggcacgatc
    26821 ggcccgatcc ggcccggcat cgacctgcct caggacttcg acctcgacga cttcctcgtg
    26881 cccgccgacc cgtggggccc gctgggcccg gtcggtcctg gtggcggcgg cggtcccggt
    26941 ccccgccccc ccggccccga gccggacccc gtcccgtggc acgagctgcg cgacctcgcc
    27001 gtccagccgg gcgagctggc gaaggccgtc gacctgctgg ccggcggcca ggtggacccc
    27061 gggcgggtgg aggaggagcg cgtgcgcacg ctgctcgccg gccc
//
LOCUS       DASVPL010000121         4957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_210030, whole genome shotgun sequence.
ACCESSION   DASVPL010000121 DASVPL010000000
VERSION     DASVPL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210030"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..2620)
                     /locus_tag="VF517_06155"
     CDS             complement(104..2620)
                     /locus_tag="VF517_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8052558.1"
                     /translation="MNETRNLAARAGRWSAQHRKTAIWGWLAFVVISLALGSAVGTKK
                     IEEAENFSGDSKAAEVIRSDSGFTDRATERVLIQSRGEQTIQDPEFRAAIADVEDGIQ
                     GSPYAVALQSPLDKGHGGQISGDNRSALVIFQLLGDRDQVGDRVDATLAATAAVQKAH
                     PELRIEQIGDASAYEAVMAAIEDDFARAETLSLPITLGILLLAFGALVAAGLPVLLGL
                     SAVMATFGLVALVSQIVPMGDSVAQVILLVGLAVGVDYSLFYIRREREERRLGKSKEA
                     ALAAASATSGRAVLVSGFTVMIAMAGMYLTGDATFKSFGTGTIMVVAVAMLGSITVLP
                     ALLAWLGDRVDRGRVPLIHRLRRGGREGGAWGWAVDRVLARPVVSVVLAGGLLAALCI
                     PAFGLKTVSSGVQGLPPDLPATKTMNRIEAAFPGGPLPAVVVVKGENVTSPATATAID
                     QLKFSALATGYMQEPIDVRFSDDRTVAEVSIPLAGNGTDAASVRALEMLREDVVPTTL
                     GKAGLEAHVTGVTAQSEDFNSLQSSRTPWVFAFVLGFAFLLLMVTFRSIVIPLKAIVL
                     NLLSVGAAYGLLVLIFQDGRLESLIGFESIGGITAWLPLFLFVILFGLSMDYHVLILS
                     RVREAYDRGMSTEDAVRHGIKATAGVVTSAAIVMVGVFGVFATLSGLDFKMMGIGLAS
                     AVLIDATIVRAVLLPASMKLLGDWNWYLPSWLDWLPHVTHEAAEAPEPVGSRLTVAIE
                     RNDGLAKIAIAGDLDLSSVDGFAAGLAEIEAEHPDVLEIDLRGLTFMDSTGLAQLFAA
                     NRRAREQGRRVVILKGPGPIDRVLDIAKVEDAIDVVTEPN"
     gene            complement(2680..3255)
                     /gene="thpR"
                     /locus_tag="VF517_06160"
     CDS             complement(2680..3255)
                     /gene="thpR"
                     /locus_tag="VF517_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX8052559.1"
                     /translation="MAKSSRSEGPRARLFLALEPSEEDRAALAAWRDALIAGREDLRP
                     VPGESLHLTLAFLGYRPEEEIDAIGSCAVEAVGTAAAVDLIPRAVKPVPPRAPRLFAL
                     DLADPGGHAAAIQSATSDALAAGGYYTPEKRPWWPHVTLARVKRGARAAPLAVEPPAG
                     PLRADRVTLYRSLLHPHGARYESQARVALSG"
     gene            complement(3233..4522)
                     /locus_tag="VF517_06165"
     CDS             complement(3233..4522)
                     /locus_tag="VF517_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX8052560.1"
                     /translation="MSARAGIVVTGTEVLTGRVRDRNGPWLSDRLLELGIDLAHITIC
                     GDRPEDMRAQLEFMAAQGIDLVITSGGLGPTADDLTMRVVGDFQGRPLALDEALEQRI
                     ADILKPMMKRWRNLDPEAIRVANRKQAFVPEGAAVLQPVGTAPGAVVPPSDGAGPTVV
                     VLPGPPRELHEMWPAATETGEFAEAVGGRTEYDQRMLRLFGIPESEIAETLRVAERDI
                     SGFGELEITTCLRRGEVEVVVRHEAAAAPAYAALEGLITTRHGETLFSTDGSSIDEQV
                     AALLAGRRVAVAESCTGGLMAARLTERAGSSEYVAGGVVAYSNEAKAALVGVDPELIE
                     RHGAVAPEVAEALADGALARFGADTAIAITGVAGPGGGTEAKPVGYVCWCVKRSDGAQ
                     LARDTRLPGDRAEVRDRSTTVGMHLLRRLLRGEELPF"
     gene            complement(4519..>4957)
                     /locus_tag="VF517_06170"
     CDS             complement(4519..>4957)
                     /locus_tag="VF517_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052561.1"
                     /translation="VLFAVIGLSGDNDEGGGKTTPTEQTSAERQARERRERRERREAR
                     ERREKHAASPRLVLRVVPTGPTYVCVDDGRGKVTFEGTIGGPRSFRARRGGRLRINLG
                     RAAVRLTANGRRVRVPDGPSGAGFSFTRTGRATPLGAEQRPCA"
BASE COUNT          696 a         1879 c         1728 g          654 t
ORIGIN      
        1 cgctcgcggc catggcgaag gacgcccgcc tcagggcggc ggcgctcgtc atcggcgcgt
       61 tcgtggtcta cacgcgcgcc gacctctggt tcggcctggc cccctagttc ggctcggtca
      121 cgacgtcgat cgcgtcctcg accttcgcga tgtcgagcac ccggtcgatc ggcccgggcc
      181 ccttgaggat caccacgcgc cggccctgct cgcgcgcgcg gcggttcgcc gcgaagagct
      241 gggccagccc ggtcgagtcc atgaacgtga gcccgcgcag gtcgatctcg agcacgtcgg
      301 gatgctcggc ctcgatctcg gccagcccgg cggcgaagcc gtccacgctg ctgaggtcga
      361 ggtcgccggc aatcgcgatc ttcgcgagcc cgtcattgcg ctcgatcgcc accgtgaggc
      421 gcgaaccgac cggctccgga gcctccgccg cctcgtgggt gacgtgcggc agccagtcga
      481 gccaactcgg caggtaccag ttccagtcgc cgaggagctt catcgacgcg ggcagcagca
      541 ccgcccggac gatcgtcgcg tcgatcagca ccgccgaggc gagcccgatg cccatcatct
      601 tgaagtcgag cccgctcagc gtggcgaaca cgccgaacac gccgaccatc acgatcgcgg
      661 cgctcgtcac gacgcccgcc gtggccttga tcccgtggcg caccgcgtcc tcggtgctca
      721 tcccgcggtc gtaggcctcg cgcacacggc tgaggatcag cacgtggtag tccatcgaga
      781 gcccgaacag gatcacgaac aggaacagcg gcagccaggc cgtgatcccg ccgatcgact
      841 cgaacccgat cagcgactcg agacgcccgt cctggaagat caggacgagc agtccgtagg
      901 ccgcgccgac cgacagcagg ttcagcacga tcgccttcag cgggatcacg atcgagcgga
      961 aggtgaccat cagcagcagg aacgcgaagc cgaggacgaa cgcgaacacc cacggcgtgc
     1021 gcgagctctg gagcgagttg aagtcctccg actgagccgt gacacccgtg acgtgcgcct
     1081 ccagcccggc cttgccgagc gtcgtcggaa ccacgtcctc gcgcagcatc tcgagcgcac
     1141 gcacggacgc cgcgtcggtg ccgttgcccg cgagtggaat cgagacctcc gccaccgtcc
     1201 ggtcgtcgct gaaccggacg tcgatgggct cctgcatgta gccggttgcg agcgcgctga
     1261 acttgagctg atcgatcgcc gtagcggtcg ccggcgaggt cacgttctcg cccttcacca
     1321 cgacgacggc cggcagcggc ccgcctggga acgcggcctc gatgcggttc atggtcttgg
     1381 tggccggtag gtcgggcggc aggccctgaa cgccggagga gacggtcttc aggccgaacg
     1441 ccgggatgca cagtgcggcc agcaggccgc cggccaggac caccgacacg acgggacggg
     1501 ccagcacgcg gtccactgcc cagccccagg ccccgccctc gcggccgccg cggcgcaggc
     1561 ggtggatgag cggcacgcgg ccgcggtcga cacggtcgcc gagccatgcg agcagcgcgg
     1621 gcagcacggt gatcgagccg agcatcgcca ccgccaccac catgatcgtg ccggtgccga
     1681 acgacttgaa cgtcgcgtcg ccggtcaggt acatcccggc catcgcgatc atgacggtga
     1741 agcccgagac gagcacggcc cgcccggagg tggcggacgc ggccgccagc gccgcctcct
     1801 tcgacttccc gagtctgcgt tcctcgcgct cgcggcggat gtagaagagc gagtagtcga
     1861 cgcccaccgc cagcccgacc agcaggatca cctgggccac gctgtcgccc atcgggacga
     1921 tctggctgac cagtgcgacg agtccgaacg tggccatgac agccgacagc ccgagcagca
     1981 ccgggaggcc cgcggccacc agcgcgccga aggcgagcag caggatgccg agcgtgatgg
     2041 gcagcgagag cgtctcggca cgggcgaagt cgtcctcgat agccgccatc acggcctcgt
     2101 acgcgctcgc atcgccgatc tgctcgatcc gcagctccgg gtgcgccttc tggaccgccg
     2161 cggtggcggc gagcgtcgcg tcgacgcggt cacccacctg gtcccggtcg ccgagcagct
     2221 ggaagatcac gagcgccgag cggttgtctc cggagatctg cccgccgtgg cccttgtcga
     2281 gcgggctctg gagcgcgacg gcatacggcg agccctggat gccgtcctcg acgtccgcga
     2341 tcgcggcgcg gaactcggga tcctggatgg tctgctcgcc gcgcgactga atgagaaccc
     2401 gctcggtcgc gcggtcggtg aagccggagt ccgaccggat cacctcggcg gccttggagt
     2461 cgccgctgaa gttctcggcc tcctcgatct tcttggtccc gacggcgctg ccgagggcca
     2521 ggctgatgac gacgaaggcc agccagcccc agatcgcggt cttgcggtgc tgcgcgctcc
     2581 agcgaccggc gcgcgcggcg aggttgcgag tctcgttcac ttcgacttcc ccttctgacg
     2641 ggtcgcctct ccgggccagc gggaggcgtc gtcgccaccc taaccgctca gcgcgacccg
     2701 tgcctggctt tcgtagcgcg cgccgtgcgg gtgaagcagc gagcggtaga gcgtgacccg
     2761 gtcggcgcgc agcggcccgg ccggaggctc gaccgcgagt ggtgcggcgc gggcgccgcg
     2821 cttcacccgc gcaagggtca cgtgcggcca ccagggccgc ttctcgggcg tgtagtagcc
     2881 gcccgcggcg agcgcatccg atgtcgcgga ctggatcgcg gcggcgtgcc cgcccgggtc
     2941 ggcgaggtcg agggcgaaca gccgcggcgc ccgcggcggg accggcttga ccgcgcgcgg
     3001 gatgaggtcg accgccgcgg ccgtcccgac cgcctcgacg gcgcacgacc cgatcgcgtc
     3061 gatctcctcc tcgggccggt agccgaggaa cgcgagcgtc aggtgcagcg actccccggg
     3121 caccggccgc aggtcctcac ggccggcgat cagcgcgtcg cgccacgcgg ccagcgccgc
     3181 acggtcctcc tcggacggct cgagcgctag gaagagccgc gcgcgcggac cctcagaacg
     3241 ggagctcttc gccacgcagg agccgccgca gcaggtgcat cccgaccgtg gtcgagcggt
     3301 cgcgcacctc ggcgcggtcg ccgggcagcc gcgtatcgcg cgcgagctgc gcgccgtcgg
     3361 agcgcttcac acaccagcac acgtagccga ccggcttggc ctcggtcccc ccgccgggac
     3421 cggccacgcc ggtgatcgcg atcgcggtgt cggcgccgaa ccgcgccagc gcgccgtccg
     3481 ccagcgcctc ggccacctcc ggagcgaccg ccccgtggcg ctcgatcagc tccggatcca
     3541 cgcccacgag ggccgccttc gcctcgttgg agtacgccac caccccgccg gccacgtact
     3601 ccgacgagcc tgcccgctcc gtgagccggg cggccatcag cccgcccgtg cacgactcgg
     3661 cgaccgccac ccgccggccg gccaggagcg cggccacctg ctcgtcgatg ctcgagccgt
     3721 cggtggagaa cagcgtctcg ccgtggcgcg tggtaatcag gccctctagc gccgcgtagg
     3781 cgggcgcggc ggcggcctcg tgccgcacca ccacctccac ctctccgcgg cgcagacacg
     3841 tcgtgatctc gagctcgccg aagccggaga tgtcgcgctc ggccacccgc agcgtctccg
     3901 cgatctccga ctccgggatg ccgaacagcc gcagcatgcg ctggtcgtac tcggtgcgcc
     3961 cgccgaccgc ctccgcgaac tcgccggtct cggtcgccgc cggccacatc tcgtgcagct
     4021 cgcgcggcgg cccgggcagg accacgaccg tcggcccggc gccgtccgag ggcggcacca
     4081 cggcaccggg tgcggtgccg accggctgga gcaccgccgc cccctcgggc acgaacgcct
     4141 gcttgcggtt cgccacccgg atcgcctccg gatcgaggtt gcgccagcgc ttcatcatcg
     4201 gcttcaggat gtccgcgatc cgctgctcga gggcctcgtc gagcgccagc gggcggccct
     4261 ggaagtcgcc caccacccgc atggtcaaat cgtccgccgt ggggccgagc ccgccgctcg
     4321 tgatcacgag gtcgatcccc tgcgccgcca tgaactcgag ctgcgcgcgc atgtcctcgg
     4381 ggcggtcgcc gcagatagtg atgtgggcca ggtcgatgcc gagctcgagc agccggtcgg
     4441 agagccacgg cccgttgcgg tcgcggaccc ggcccgtgag cacctcggtc ccggtgacga
     4501 cgatgccggc ccgcgcgctc acgcgcaggg cctctgctcg gcgcccagcg gcgtggccct
     4561 gcccgtgcgc gtgaacgaga agccggcgcc ggacgggccg tcggggaccc gcacgcgcct
     4621 gccgttcgcc gtcagccgca cggcggcgcg gcccaggttg atgcgcaggc gcccgccgcg
     4681 gcgcgcgcgg aagctgcgcg gcccgccgat cgtgccctcg aaggtcacct tcccgcggcc
     4741 gtcgtccacg cagacgtagg tcgggccggt gggcaccacg cgcagcacga gccgcggcga
     4801 cgccgcgtgc ttctcacggc gctcgcgggc ctcgcgacgc tcacgccttt cgcgccgctc
     4861 ccgcgcttgg cgctcggcgc tcgtctgctc ggtgggggtg gtcttgccgc cgccctcgtc
     4921 attgtcgccg ctcaggccga tcaccgcgaa cagcacg
//
LOCUS       DASVPL010000122        23017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_171375, whole genome shotgun sequence.
ACCESSION   DASVPL010000122 DASVPL010000000
VERSION     DASVPL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 23017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23017
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171375"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..290
                     /locus_tag="VF517_06175"
     CDS             90..290
                     /locus_tag="VF517_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052562.1"
                     /translation="MAEEGVRAALRTAAIQSPSRLTSGVPTLYTPGKTGCSRPRWSRY
                     VIARGDMPNASSCPRVTIPCCL"
     gene            complement(399..764)
                     /locus_tag="VF517_06180"
     CDS             complement(399..764)
                     /locus_tag="VF517_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052563.1"
                     /translation="MGRFETAGKFGIVALIALVLFLLPGGGNALDVVLAVLSIAFFTL
                     IALLAARLYREHRFTIESLSLTERGVAYASIGAAFVDFTATRRLFNLGGGGVLIWLAI
                     LGLCSYGIYWVWRHSTQYG"
     gene            complement(767..1531)
                     /locus_tag="VF517_06185"
     CDS             complement(767..1531)
                     /locus_tag="VF517_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX8052564.1"
                     /translation="MADEPLTCFVEIPKGSRNKYEFDHEAGGIKLDRFLFSSMVYPTD
                     YGFVPDTLGLDGDPLDVMICVSEPTFPGCLIDVKPIALFRMEDDKGVDDKVLCVPLQD
                     PAWNTLEELDDLPDQLRQEIAHFFSVYKDLEQKAVKVDGWYSRQDALTEIEAARKRCE
                     EHRASGDGGARAEGDALESSTPLESLSDDPAENAPGRSGDDGDRDREGADRDGAGAAS
                     SGAAAGSSASSGSVAGPSESSGSAAGPSESSGSAGG"
     gene            1569..1823
                     /locus_tag="VF517_06190"
     CDS             1569..1823
                     /locus_tag="VF517_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052565.1"
                     /translation="MIRRRLMLVAFAVATQLYLAAPALAGDDGEGLAGETNDKIITFF
                     ALGVLLFLTLVVIIGTIIQDRLNKRYYARLESRKRDPAGW"
     gene            1988..2758
                     /locus_tag="VF517_06195"
     CDS             1988..2758
                     /locus_tag="VF517_06195"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8052566.1"
                     /translation="MSSPPVTYERTGAAAVLTIDRQERRNAVDGPTAALLGEGYQRFV
                     EDDGARVLVLTGAGDVAFCAGADLKAIETFGPRLGLPEGPLGFSRVTPPKPTIAAISG
                     WCLAGGLELALWCDLRIATAGSTLGFPERRFGVPLIDGGTQRLPRIVGLGRALDIILT
                     GRFVPAEEALAMGLVNELVPPGTHLDRALALAEALAAFPQETMLADRRSAIEGLGMTL
                     EEGLKLEATAGPEVFGAAQQGAARFASGTGRHGEGAGA"
     gene            2801..3649
                     /locus_tag="VF517_06200"
     CDS             2801..3649
                     /locus_tag="VF517_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8052567.1"
                     /translation="MTEAAISVRDLRKSYGPLEAVRGISFEVRRGEVFGLLGPNGAGK
                     TTTVEILEGYRRRSGGEIAVLGRDPEERDVELRKRVGIVLQSSGFHPRATPRELVELT
                     ASAYPAPRDPAETLALVGLSDEEDKHTKTLSGGQRRRLDLALALVGDPEVVFLDEPTT
                     GFDPAARRTAWDAVRALKDLGKTVLLTTHYLDEAQALADRVAIVKDGRVIAQGPPSEL
                     QPERSRYRVAYSLDGDRVEHETDDPTELLHRLTGEAIEKGERLENLSVGRPTLEDVYM
                     ELTAEE"
     gene            3646..4383
                     /locus_tag="VF517_06205"
     CDS             3646..4383
                     /locus_tag="VF517_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8052568.1"
                     /translation="MSTAALTWRQFRLERRMFWRNPTAAFFNFLLPLLFLFLIATAFG
                     SDRDDLEVLVPGIAGMAIMTTTFTALAFNVTFLREQGVLKRIYGTPMPAASYFGGLIG
                     SAVLNAVAQVVLVVLIGHLVYDLGWPRDWLELIAFTGVGVVAFASLGIAFSHLIPNYD
                     AAAAYVNAVFLPLIFISGTFYSTKELPSFLDGIASVLPLKHAIDGLRDAMVIGDGLGS
                     EAGHLLALAAWAAVGVILAIRRFRWEQ"
     gene            complement(4346..4969)
                     /locus_tag="VF517_06210"
     CDS             complement(4346..4969)
                     /locus_tag="VF517_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8052569.1"
                     /translation="MVVRGPSFSLRYLVPGDAHALFELARDPEVTQFFSWGPYRDESE
                     PLAFIERLEREREAGVRLELGIVDSDDRPIGLTGFSEFAHRDRRATVGTWLGREHWGS
                     GANTESKALVLALGFRHLGLNRITALASPDNRRSLRALERIGFWTEGVLRAWHIHGGE
                     PRDVAILRMLRDRYESSDLSKLPYEIEGEPPAAFVVTAPSGTGESPG"
     gene            complement(4980..5786)
                     /locus_tag="VF517_06215"
     CDS             complement(4980..5786)
                     /locus_tag="VF517_06215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX8052570.1"
                     /translation="MTAIPYAIRRSDRARRARIVVGSDGVEVVVPRRMPLREVEPFVA
                     EKEAWIQRTLRRYERAAAGGPSVDLRDGGGVPYLGRELALRVVVEPRRVRAHVALREV
                     GQAGRAPSGEAPPPPPVDASPPPPAGASRPGQLELAPPEELVVKVGAPGEDAVRDALE
                     RWYRRRAHAEIAPRLDAATARAGTSYSRLTIRSQRTRWASCSDGGSMSFNWRLLLAPA
                     EVLDYVVEHEVAHLEVMGHSRRFWSLVERRCPDYRRHERWLRRYGSLLRL"
     gene            complement(5783..7180)
                     /locus_tag="VF517_06220"
     CDS             complement(5783..7180)
                     /locus_tag="VF517_06220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8052571.1"
                     /translation="MASRVVMALMFFPRGGSSQVARYVARGLPDAGWDVTLVAGSLGE
                     EGEQSHAGTFFSGLEVQALDYSEAAEADDPLAHDPPFQPSFEDREDAPDRVFAAVDDA
                     TYERLVAVWERELERAGAGEADVLHLNHLTPMNEAAARSFPDVPVIGHLHGTELLMLK
                     AIEDGPPDGWDHAEAWAERMRGWAKRCERLLVLSPDAVERVPGLLDVEPERVVWAPNG
                     FEPESFDRRPVTGDERVALWRRWLVEEPRGWEPDGDAGSVSYGEEDLDAFRDGPVLLY
                     VGRYTEVKRIPLMIRAYARAQERFGRRAPLVLLGGYPGEWEGEHPIDVVRAAGARDVF
                     LAGWRGHEDLPDGLNAADATVLASVHEQFGQVLVEGMACGLPPIAVDAHGPATIVDDG
                     ETGWLVPADDEDALCEALVELVSDDDERRRRGEAAYGVARERYSWPSLIERLASVYDE
                     VRDGRRPDAGVSSLA"
     gene            complement(7207..8868)
                     /locus_tag="VF517_06225"
     CDS             complement(7207..8868)
                     /locus_tag="VF517_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052572.1"
                     /translation="MGRLLPAALVLLTVLGAPVASRAAWQSPGVGGQSARAITLPAGA
                     TPSASVSNRSVTVSWSATTLPGGAAVAGYVVRRYSSTGTVQTIGAGCAGTVTAASCTE
                     TDLPAGSWRYSVEPKQGNWTGPESGQSTAVSVAAPGLTLAPASVTSLPATLSGSLASF
                     KPGQTVTVRLDDASSGTVLTSTVAPATIPSSGSATVSATLPVGVSNGSHTIYAVGSAG
                     DVASAPVAVSSTTTITTSAWSLRDVSTTTLVDESAPSAFSGDGRVTTTGNWAPTFAAT
                     RYVDFDLSTALPAGKAVSGATFDYRRAASATSDTNCFYFEVRRISTGAVLAAHGSAAS
                     PVGCVTGTAQTGSSIALPELTTTDLVDDARIRVYASSSGSRGTNVDVATLSGTAGSTP
                     FTLYATQHADAAAGGAATAFPWSQAALDGTPLDAGGNWSNTFQTSRYLQVTFPAYVPS
                     GATVSSATLRHAYRPAAAGNTACWYAEAYSGGSLIATYGSSATPVGCNSSATTYTTDS
                     LPLPEVNTAARANDLTIRLYFRGSANGNGRRTQHDLTDLTIAYAD"
     gene            complement(8880..9341)
                     /locus_tag="VF517_06230"
     CDS             complement(8880..9341)
                     /locus_tag="VF517_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052573.1"
                     /translation="MSRKLAVMTMVMALAAVGLVYAAWTTTGAGSGYAKAGTAQALTT
                     VDVSASTVATLYPGGPAGDVLIRISNPNSYPVTVTGVAGSGAITPDSGHASCTTTGVS
                     FTDKTGLSIAVAAGGQTTATLSGAASMTNASVNGCQGATFTIPVNLSGASG"
     gene            9489..9986
                     /locus_tag="VF517_06235"
     CDS             9489..9986
                     /locus_tag="VF517_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052574.1"
                     /translation="MDRSRRIAALACALALPLSAAALAAAQEPAAPAGEPAAPAVDPP
                     AAAPAPDVTAPRLLLGGRRAQHLAPHLEADGSCDEYCEFEISARVYGVPGLRQVDVLT
                     PDKAGEGGARMHFHILVSPRAHRLMNDALRDGRRVAVALNVIAYDLADNQTAGRRWIR
                     VRPPA"
     gene            complement(10003..10812)
                     /locus_tag="VF517_06240"
     CDS             complement(10003..10812)
                     /locus_tag="VF517_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052575.1"
                     /translation="MDLGLAGRACVITGASSGIGLATARALAGEGARVLLVARDEERL
                     REAASAARDSAPASDPAAAVLAIDVTGADAGDRIVTECVERFGSIDVVVNNAGTSRMS
                     GLDELSDDDWREQLDLHVMAPLRLMRAAAPRMADAGWGRIVNVCSSSGKRPSLSNAAY
                     SVSKAAQLSLSRVFADTYAARGVLVNAVAPGPVDTPLWLGEGGLADQAAAAKGSSREE
                     ALEGARAKIPLGRFGTEEEIASVIAFLCSERASDVAGAAWSVDGGSVPTIV"
     gene            10830..11291
                     /locus_tag="VF517_06245"
     CDS             10830..11291
                     /locus_tag="VF517_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX8052576.1"
                     /translation="MRSHTVYRTFNTARRREFVRITEDVQQAVDDAGIAEGMALVSAM
                     HITAGVWVNDDEPGIQADALEWLDKVAPPSWQPPANDVARELLPEGGDYRHHRGGEDN
                     GDAHLKNMLVHHQVIVPVTAGRLDLGPWQAIFYAEFDGQRAKRLIIKVLGE"
     gene            complement(11299..11898)
                     /locus_tag="VF517_06250"
     CDS             complement(11299..11898)
                     /locus_tag="VF517_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX8052577.1"
                     /translation="MKEASEMGKLDGKKIAFLAADGVEQVELTEPRKAVEAEGADTEL
                     ISLEEGQIQGFEKLDHGEKFDVDRTVADADASDYDGLVLPGGVHNPDLLRADEGATAF
                     VRAFFEAGKPVGAICHGPWTLIDAGVVDGLKLTSYPSIQADLRNAGAEWVDEEVVVDR
                     GLTTSRNPDDIPAFNAKIVEEFCEGEHEELAEATSGASA"
     gene            complement(11914..13308)
                     /locus_tag="VF517_06255"
                     /pseudo
     CDS             complement(11914..13308)
                     /locus_tag="VF517_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
     gene            complement(13331..15958)
                     /locus_tag="VF517_06260"
     CDS             complement(13331..15958)
                     /locus_tag="VF517_06260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX8052578.1"
                     /translation="MATAERAAGGDAAAPMRPLTAWAREQRIQARWVLIGAAVTTLIA
                     ALDALVGERAVITGMIVAGPLLASFGASARSTGLVTLYATALALVVGIPNDMFLDPGE
                     HLTRVATVVVGGAIATWIAHERHRERVARRRYALLVGASDIVQRTLEPEVMTIEVARL
                     LAHELGDWCFVFMRDEEGGVRQVAAMHRDPERQRLAWELLQRYPMKEERAEGPSATMR
                     DGEARLYAHVDERLLESISAGEENLRMLERLGMRSAVVVPLSAHGRTFGALAVAAAES
                     GQTYDEGDLALAEELAGRVATAVDSAHLYSELSGTESDLRRSRDELQAILDGVADAVT
                     AQGRGAHLVFANQAAVESFGFDSIEELLATPAIEIANRFEVTDGDGAPIPVEDLPGRK
                     ALAGDPAPEPILTRFRLKGGHSERWVRVKALPVFDESGAAVLAINVMEDVTEEREAAE
                     AQRFLSEAGRILSSSLDYDTTITNVARLAVPRIGDWCSVDVLEEDGTTRPVVVAHVDP
                     EKVEWAIELRNKYPPDPREERGVYRVLRTGESELYPSIPRELLAEAAVDEEHMRLIDE
                     IGMTSAMIAPLTARGRVLGAITFVSAESGRAFDDGDLLLAQELAVRCAIAVDNARLFG
                     ERTHIARTLQESLLPPELPQPPGLEVAARFRAAGEGYEVGGDFYDVFDTGSDRWAAVI
                     GDVCGKGPEAAAITALARYTLRATAMSEDVPSRILGTLNEAMLRQRTDRRFSTVLYAC
                     LDQREDGTCLRFASGGHPLPILLRASGGAGEVGTPGTLLGIVPDPDLVDATVDLKPGD
                     VVVLYTDGVTDAAAPDLIHEPEELAAVLEAARYESADTIAERMLQLALGASNGSGPRD
                     DIAILVIKVPERATAALSQ"
     gene            complement(16048..16650)
                     /locus_tag="VF517_06265"
     CDS             complement(16048..16650)
                     /locus_tag="VF517_06265"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX8052579.1"
                     /translation="MNARFRIRTIALAVCAALTIGSGAALAADSGSGSQSADAAPTLK
                     LGSTGASVKAVQRRLRVTATGYYGRQTVAAVKRFQRSRHLTADGIVGAATARALGVKI
                     GRASYATGGAAPSGSDGTTTRRVTLPAVLKRIAECESGGNPRAVSRDGRYRGKFQFDV
                     ATWRALGGSGDPAKAAESVQDRLALKLYRQRGTAPWANCA"
     regulatory      complement(16662..16834)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            16914..17678
                     /locus_tag="VF517_06270"
     CDS             16914..17678
                     /locus_tag="VF517_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX8052580.1"
                     /translation="MNFNSLITGPARLGLTLAARGLGLVRGRFGTTQVDDTTLQRSVE
                     AEVFASRRAPKGQIEVNVTEGVVWLRGEVKTQSAVNELESRARSVEGVARVENLLRVA
                     KPAKSAKPAARAKTQAKTQKRPAAKRSSRPKPAAATAPPPQAEPVADPTPPPAAQRER
                     KVTRRFNAERTAGSEAEPTPRDLSDAGKGRQPAPLGADEPASPATAGAAAAAGEAPAT
                     GSREPAGVGSPAAPFPTTGGGSGSSENGSTGNGGSA"
     gene            17757..18116
                     /locus_tag="VF517_06275"
     CDS             17757..18116
                     /locus_tag="VF517_06275"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX8052581.1"
                     /translation="MAAEPDSDRGYRIAFEALERGTRVQTADGEELGTVKKVMIVHEK
                     HLFDGIVVKTGSGDRFVDAPEVDEIFERLVTLKISAAEAAELPKPGANPAAMRLSAGD
                     FRPGGARRLWNRLSGRR"
     gene            complement(18120..18803)
                     /locus_tag="VF517_06280"
     CDS             complement(18120..18803)
                     /locus_tag="VF517_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052582.1"
                     /translation="MSGLREIGDGLWVHEAPLRFLGVEAGRIMNVVRLAAGGLFVHSP
                     APLDDALRAGLAALGEVRFVAAPNKLHGHRWMEQYSAAYPGVELLAGPGLRRRRKDLS
                     FAADLRDEPDPRWAGELDQAPFRGNVLMPEIEFLHRASRTLILGDLAMNFGADSPPAT
                     RAVARLGRMYGRLRPTPLARAVIRRSTGRRDVERILDWDFDRVIPGHGAIWESGGREA
                     LRREWSRGR"
     gene            complement(18800..19672)
                     /locus_tag="VF517_06285"
     CDS             complement(18800..19672)
                     /locus_tag="VF517_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8052583.1"
                     /translation="MIATERIDEWFAAGERVEAGGLRLFERVAGSEGAPWLTLLHGFP
                     TCSWDWSPVADGLAAARRLLMFDFAGFGDSDKPRDHVYSIHEQADLTEALWRRHGIER
                     TALVVHDYGVSVGQELLARRAEGALEAEIERIAFLNGGLYPDLHRPLIAQRLLLHPTA
                     GPMFSRTVNERAFLRSLTRVFAHRPSDADLHQHWLGIARHGGARIQHLLIRYIEDRRR
                     HAERWTAALERTDVPRRFVWGMLDPVSGAHMAERIAARMPPAELHALADAGHYPQLEA
                     PLRAGLLLREFVAP"
     gene            19733..20395
                     /locus_tag="VF517_06290"
     CDS             19733..20395
                     /locus_tag="VF517_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8052584.1"
                     /translation="MRLLLPLLAAIAVLVSAGLALAGPSNSCAPVRVAAMTCIGTDSA
                     EWLTTGDGDDTLDGRGGADRLNARGGDDVAYGGSGDDNAIHGGTGNDRLYGQDGRDVV
                     LGDEAGAGGADLVSGGSGSDRVFGMAGADDVRGDSGNDVVHGGDGRDLVSGGDGADVL
                     DDAARSHQDPDSFSGGGGNDEIYSRDGKRDTVKCGSGRDVAKADSHDKVASDCERVIR
                     VG"
     gene            complement(20521..21393)
                     /gene="ligD"
                     /locus_tag="VF517_06295"
     CDS             complement(20521..21393)
                     /gene="ligD"
                     /locus_tag="VF517_06295"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8052585.1"
                     /translation="MNVRVSSPDKVMFPGKNGKGITKGELAAYYESVGSVMVPHVRDR
                     PLTMHLYPRGIEAEGVYMKQAPKHFPDWIKRVEVAKRGGTVSHVVANRPETLVYLAGQ
                     NCVTPHAWLSRADKIEKPDRLIFDFDPEHERFAEVRAAALQLGDVLRDLGLAPHAMTS
                     GSRGLHVTVPLRRTRTFEEVRAFAEAIGVLLVEQDPKRLTMVWSKAKRGDRIYVDVGR
                     NAYAQHAVAPYGVRAKPDAPVATPLRWEELDDPKLLSTSYTVRTVPDRLGREGDPWAQ
                     IAKRAKALPPIPQP"
     gene            complement(21377..22261)
                     /gene="ligD"
                     /locus_tag="VF517_06300"
     CDS             complement(21377..22261)
                     /gene="ligD"
                     /locus_tag="VF517_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8052586.1"
                     /translation="MKAMAATLTDARFDDPDWIFERKLDGIRCLATTRPHPDLRSRND
                     LDLTGRFPTVAAALASFPSAVLDGEVVAFSGSQTSFQRLGERGREVFFYVFDVLEAEG
                     EDVRELPLMERKAVLRRLVKPGGPIRLTPYRRASGTALYEEACRRGWEGLIAKRADSP
                     YVGKRSRDWLKWKCDAEQELVIGGYTAPKGSRIELGALLVGHWEDGRLRYAGKVGTGF
                     GRETLRDLAARLAPLRRDDTPFADSPRLRSATWVEPRLVAQVGFTEWTRDGRLRHPRY
                     MGLREDKNPREVVRERPS"
     gene            complement(22258..22743)
                     /locus_tag="VF517_06305"
     CDS             complement(22258..22743)
                     /locus_tag="VF517_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8052587.1"
                     /translation="MAEPVYVIQKHDATSLHYDFRLEVDGAMRSWAVPKGPSTDPRDK
                     RLAMQVEDHPLSWNDFEGVIGGGYGKGAVIVWDRGTYRNLNDVPMHEALARGHASFWL
                     DGKKLRGGWSLRRTHEGAKPKWLLIKKRDEEADARRRPTSTQPESVVSGRTIEEVAER
                     G"
     gene            complement(22736..>23017)
                     /locus_tag="VF517_06310"
     CDS             complement(22736..>23017)
                     /locus_tag="VF517_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX8052588.1"
                     /translation="FEIDACKPWLEEELKLLSPEAVVLLGATAGKAILGASFRVTQQR
                     GRPVESDLAPLVTATIHPSAILRAPDDDARQAEREAFAADLRLVADALG"
BASE COUNT         3154 a         8610 c         8193 g         3060 t
ORIGIN      
        1 cttcaccgca ggccggcgct gcggcggacg gtcacgtgga ttgccccgcg gcgcctcctc
       61 gtcaagccgt acagcgcaag ggcgtcctca tggctgagga aggcgtccgg gccgcactca
      121 ggaccgcggc catccagagc ccgagccggc tcacgtcggg tgtgccgacc ctgtacacgc
      181 cggggaagac ggggtgcagc cgcccgcgct ggagccggta cgtgatcgct cgcggcgaca
      241 tgccgaacgc gagcagctgc ccgcgcgtga ccatcccgtg ctgcctctga gcgagcgcga
      301 acacctcgcg ctgggatggt tgacgagttt gggacgccat ggtggcccaa agtcgtcaag
      361 gaacccgaac tcgccccccc cgaggggccg aagcggccct acccgtactg cgtcgagtgg
      421 cgccagaccc agtagatccc gtacgagcag aggcccagga tcgccagcca gatcagcacg
      481 ccgccgccgc ccaggttgaa caggcgccgg gtggccgtga agtccacgaa cgccgcgccg
      541 atcgacgcgt acgccacgcc gcgctcggtg agagacagcg actcgatcgt gaagcggtgc
      601 tcgcggtaga gccgcgccgc cagcagtgcg atcagcgtga agaacgcgat cgagagcacc
      661 gccagcacca cgtcgagcgc gttcccaccg cccggcagca ggaacagcac gagcgcgatc
      721 agcgccacga tcccgaactt gccggctgtc tcgaagcgtc ccacggttat cccccggccg
      781 atccggagga ctccgagggg ccggcggccg atccggagga ctccgagggg ccggcgaccg
      841 atccggagga cgccgaggag ccggcggccg cgccggagga ggcggccccc gccccatccc
      901 ggtcggcacc ctcgcgatcg cgatccccat cgtcgccact gcggcccggc gcgttctccg
      961 ccgggtcgtc gctgagcgac tcgagcggcg tcgaggactc caaagcgtcg ccctccgccc
     1021 gcgcgccgcc gtcgccgctc gcccgatgct cctcgcaccg cttccgcgcc gcctcgatct
     1081 ccgtaagcgc gtcctggcgc gagtaccagc cgtccacctt cacggccttc tgctcgaggt
     1141 ccttgtagac cgagaagaag tgcgcgatct cctggcggag ctggtcgggc aggtcgtcga
     1201 gctcctcgag cgtgttccac gcggggtcct gcaagggcac gcagagcacc ttgtcgtcga
     1261 cccccttgtc gtcctccatc cggaacagcg cgatcggctt cacgtcgatc aggcagcccg
     1321 ggaaggtcgg ctccgacacg cagatcatca cgtccagcgg gtcaccgtcg agccccaggg
     1381 tgtcggggac gaacccgtag tccgtgggat agaccatcga ggagaagagg aagcgatcga
     1441 gcttgatccc accggcctcg tggtcgaact cgtacttgtt gcggctcccc ttcgggatct
     1501 cgacgaagca ggtgagcggc tcgtcggcca tcggtaacgg gcgaggttac cggtagaatc
     1561 cagtgccgat gatccggcgc aggctcatgc tcgtcgcatt cgctgtggcg acccagctct
     1621 acctcgcggc acccgcactc gcgggcgacg acggcgaggg cctcgcgggc gagacgaacg
     1681 acaagatcat caccttcttc gcgctcggcg tgctgctgtt cctcacgctc gtggtgatca
     1741 tcggcacgat catccaggac cggctgaaca agcgctatta cgcgcgcctc gagtcgcgca
     1801 agcgcgaccc cgccggctgg tagacgccta aaaagaggcg cggccccggg cgggggcccc
     1861 ggagccgcgc gaacgacgag agggagggat ccgcgctcgt cgagaacacc atgttgccca
     1921 ccggctctaa gccgacgcta aatcacgcga aaccggcgcc gacccgctag ctgcgaagat
     1981 cccgcccgtg tccagcccgc ccgtcaccta cgagcgcacc ggcgccgccg cggtcctcac
     2041 catcgaccgc caggagcgcc gcaacgccgt cgacggcccc accgccgccc tgctcggcga
     2101 gggctaccag cgcttcgtcg aggacgacgg cgcgcgcgtg ctcgtcctca ccggcgccgg
     2161 cgacgtcgcg ttctgcgcgg gcgccgacct caaggccatc gagaccttcg gcccgcgcct
     2221 cggcctcccc gagggcccgc tcggcttcag ccgcgtcacc ccgcctaagc ccaccatcgc
     2281 cgcgatctcc ggctggtgcc tggccggcgg cctcgagctg gcgctgtggt gcgacctgcg
     2341 catcgccacg gccggctcca cgctcggctt tccggagcgc cgcttcggcg ttccgctgat
     2401 cgacggcggc acccagcgcc tcccgcgcat cgtgggcctt ggccgcgcgc tcgacatcat
     2461 cctcaccggc cgcttcgtgc cggccgagga ggcgctcgca atggggctgg tgaacgagct
     2521 ggtcccgccg ggaacccacc tcgaccgcgc gctcgcgctg gccgaggcgc tggcggcgtt
     2581 cccgcaggag acgatgctcg ccgaccggcg ctccgccatc gagggcctgg ggatgaccct
     2641 cgaggagggc ctgaagctcg aggcgacggc cggacccgag gtcttcggcg ccgctcagca
     2701 gggcgccgcg cgcttcgctt ccggcaccgg ccgccacggc gagggcgccg gcgcctgagc
     2761 gcacccgggc cctccgcgcg cccgctggca tagttggacc gtgacggaag ccgccatcag
     2821 cgtccgcgac ctgcgcaaga gctacggccc gctggaggcg gtgcgcggga tctcgttcga
     2881 ggtgcgtcgg ggcgaggtct tcggtctgct cggcccgaac ggcgccggga agaccaccac
     2941 cgtcgagatc ctcgagggct accgcaggcg cagcggcggc gagatcgccg tgctcggccg
     3001 cgacccggag gagcgcgacg tggagctgcg caagcgggtc gggatcgtcc tccagtcgag
     3061 cggcttccac ccgcgggcga ccccgcgcga gctggtcgag ctgaccgcct ccgcgtaccc
     3121 cgcgccgcgc gacccggccg agaccctcgc gctcgttggc ctcagcgacg aggaggacaa
     3181 gcacacgaag accctctccg gcggccagag gcgccgtctc gacctggcgc tggcgctcgt
     3241 cggcgacccg gaggtcgtgt tcctggacga accgaccacc ggcttcgacc ccgccgcccg
     3301 gcgcaccgcc tgggacgccg tccgcgccct gaaggacctc ggcaagaccg tcctcctcac
     3361 cacccactac ctggacgagg cccaggcgct tgccgaccgc gtcgccatcg tcaaggacgg
     3421 ccgcgtgatc gcgcagggcc cgccgtcgga gctccagccc gagcgctcgc gctaccgcgt
     3481 ggcctacagc ctcgacggcg accgcgtcga gcacgagacc gacgacccca ccgagctgct
     3541 ccaccgcctc acgggcgagg cgatcgagaa gggcgagcga ctcgagaacc tgagcgtcgg
     3601 gcgcccgacg ctcgaggacg tctacatgga gctcactgcc gaggaatgag caccgccgca
     3661 ctcacctggc gccagttccg gctcgagcgg cggatgttct ggcgcaaccc gactgccgcc
     3721 ttcttcaact tcctgctgcc gctgctgttc ctgttcctga tcgcgaccgc gttcggctcc
     3781 gaccgcgacg acctcgaggt gctggtcccc ggcatcgccg gcatggcgat catgaccacc
     3841 accttcaccg cgctcgcgtt caacgtcacg ttcctgcgcg agcagggcgt gctcaagcgg
     3901 atctacggca ccccgatgcc cgccgcgtcg tacttcggcg ggctgatcgg gagcgcggtc
     3961 ctgaacgcgg tcgcgcaggt cgtgctcgtg gtgctcatcg gccacctggt ctacgacctc
     4021 ggctggccgc gcgactggct cgagctgatc gcgttcaccg gcgtgggggt cgtggcgttc
     4081 gcttcgctgg ggatcgcgtt ctcgcacctg atcccgaact acgacgccgc cgcggcctac
     4141 gtcaacgccg tcttcctacc gctgatcttc atctcgggca ccttctactc gacgaaggag
     4201 ctgccgtcct tcctcgacgg gatcgcgagc gtcctgccgc tcaagcacgc gatcgacggg
     4261 ctgcgcgacg cgatggtgat cggcgacgga ctcggctcgg aggccgggca cctgctcgcg
     4321 ctggcggcgt gggccgccgt gggggtcatc ctggcgattc gccggttccg ctgggagcag
     4381 tgaccacgaa cgccgcgggt ggctcgccct cgatctcgta ggggagcttc gacaggtcgc
     4441 tggactcgta gcggtcgcgc agcatgcgca ggatcgcgac gtcgcgcggc tcgccgccgt
     4501 ggatgtgcca ggcgcgcagg acgccctcgg tccagaaccc gatccgctcg agcgcgcgca
     4561 gcgagcggcg gttgtcggga ctcgcgagcg cggtgatgcg gttgaggccc agatggcgga
     4621 agccgagcgc gagcaccagc gccttcgact cggtgttcgc gccgctgccc cagtgctcgc
     4681 ggccgagcca ggtcccgacg gtcgcgcgac ggtcgcggtg ggcgaactcg ctgaagccgg
     4741 tcaggccgat cgggcggtcg tccgagtcga cgatgccgag ctcgagccgc accccggcct
     4801 cgcgctcgcg ctcgaggcgc tcgatgaacg cgagcggctc cgactcgtcg cggtagggac
     4861 cccacgagaa gaactgcgtg acctccggat cgcgggcgag ctcgaacagg gcgtgagcat
     4921 cgccgggcac gaggtacctc aggctgaacg atgggccgcg gacgaccaca ccggggagac
     4981 tagaggcgca gcaagctgcc gtagcggcgc agccagcgct cgtggcgccg gtagtcgggg
     5041 cagcgacgct ccacgagcga ccagaaccgg cgcgagtggc ccatgacctc gaggtgcgcg
     5101 acctcgtgct cgaccacgta gtcgagcacc tccgcgggcg cgagcaggag tcgccagttg
     5161 aagctcatcg agccgccgtc ggagcagctc gcccagcgcg tgcgctgcga tcggatcgtg
     5221 aggcgcgagt aggaggtgcc ggcgcgagcg gtggcggcgt cgagccgcgg ggcgatctcc
     5281 gcgtgcgcgc ggcggcggta ccagcgctcg agcgcgtcgc ggacggcgtc ctcgccgggt
     5341 gcgcccacct tcacgacgag ctcctcgggg ggcgcgagct cgagctggcc gggccgcgaa
     5401 gcccccgcgg ggggcggcgg cgaggcgtcc acggggggcg gcggcggggc ctcgccggac
     5461 ggcgcccgcc ccgcctggcc cacctcccgc agcgccacgt gcgcccgcac ccgccgcggc
     5521 tcgacgacca cgcgcagcgc cagctcgcgg ccgaggtacg gcacgccgcc gccgtcgcgc
     5581 aggtccacgc tcgggccacc ggccgccgcg cgctcgtagc ggcgcagcgt gcgctggatc
     5641 cacgcctcct tctcggccac gaacggctcc acctcgcgca gcggcatccg gcgggggacc
     5701 accacctcca ccccgtcgct cccgaccacg atccgggctc gccgggcgcg gtcggagcgt
     5761 cgtatggcat acgggatcgc cgtcacgcga gcgacgatac gcccgcgtcc ggtcgccgac
     5821 cgtcgcgcac ctcgtcgtac accgaggcca gccgctcgat cagcgacggc cacgaatacc
     5881 gctcccgcgc caccccgtag gcggcctcgc cgcggcggcg gcgctcgtcg tcgtccgaca
     5941 cgagctcgac cagcgcctcg cagagcgcgt cctcgtcgtc ggcgggcacg agccaaccgg
     6001 tctcgccgtc gtccacgatc gtcgccggcc cgtgcgcgtc caccgcgatc ggcggcagcc
     6061 cgcaggccat cccctcgacc agcacctgcc cgaactgctc gtgcaccgag gcgagcaccg
     6121 tcgcgtcggc cgcgttgagg ccgtccggga ggtcctcgtg cccgcgccag ccggccagga
     6181 agacgtcacg cgctcccgcc gcgcgcacca catcgatcgg gtgctcgccc tcccactccc
     6241 ccgggtagcc gccgagcagc accagcggcg cgcgccggcc gaagcgctcc tgcgcgcggg
     6301 cgtacgcgcg gatcatcagc gggatccgct tgacctccgt gtagcgcccg acgtagagca
     6361 gcaccggccc gtcgcggaag gcgtcgaggt cctcctctcc gtaggacacg ctgcccgcgt
     6421 cgccgtccgg ctcccagccg cgcggctcct ccacgagcca gcgccgccac agggccacgc
     6481 gctcgtcgcc cgtgaccggg cggcggtcga agctctccgg ctcgaagccg ttcggcgccc
     6541 acaccacgcg ctcgggctcc acgtcgagca gccccggcac ccgctcgacc gcgtcgggac
     6601 tgagcaccag gagccgctcg cagcgcttcg cccagccgcg catccgctcg gcccaggcct
     6661 cggcgtggtc ccacccgtcc ggcggcccgt cctcgatcgc cttcagcatc aacagctcgg
     6721 tgccgtgcag gtggccgata acgggcacgt cggggaacga gcgcgccgcg gcctcgttca
     6781 tcggcgtgag gtggttgagg tggagcacgt cggcctcccc cgcgccggcc cgctcgagct
     6841 cgcgctccca caccgccacc agccgctcgt aggtcgcgtc gtccacggcg gcgaacacgc
     6901 gatccggcgc gtcctccctg tcctcgaacg acggctggaa cggcgggtcg tgcgccagcg
     6961 ggtcgtcggc ctcggcggcc tcgctgtagt cgagcgcctg cacttcgagg ccggagaaga
     7021 aggtgccggc gtgcgactgc tcgccctcct cgccgagcga cccggccacc agcgtcacat
     7081 cccagcccgc gtccggcagc ccgcgcgcga cgtagcgcgc cacctgcgac gagccacccc
     7141 gcgggaagaa catcagcgcc atcaccacgc ggctcgccac gagccgaccc tagcgggcga
     7201 ccccgctcag tcggcgtacg cgatcgtcag gtcggtgaga tcgtgctggg tgcggcgccc
     7261 gttgccgttg gcgctgccgc ggaagtacag gcggatcgtc aggtcgttcg cgcgcgcggc
     7321 cgtgttcacc tcgggcagcg ggagcgaatc ggtcgtgtac gtcgtggccg agctgttgca
     7381 gcccaccggc gtggccgagc tgccgtaggt cgcgatgagc gagccgccgg agtacgcctc
     7441 cgcgtaccag caggccgtgt tgccggccgc cgccggacgg tacgcgtgcc gcagcgtggc
     7501 ggagctgacg gtggccccgc tcgggacata ggccgggaag gtcacctgga ggtagcgact
     7561 cgtctggaac gtgttggacc agttcccgcc cgcgtcgagc ggcgtgccgt cgagcgccgc
     7621 ctgcgaccac gggaacgcgg ttgcggcgcc gccggccgcg gcgtccgcgt gctgggtggc
     7681 gtagagggtg aacggcgtcg agccggccgt gccgctcagg gtggcgacgt cgacgttggt
     7741 cccacgcgag ccggacgagc tcgcgtacac gcggatgcgc gcgtcgtcga ccaggtcggt
     7801 cgtggtcagc tcggggaggg cgatggagct gcccgtctgc gccgtcccgg tcacgcatcc
     7861 gaccgggctg gccgcgctgc cgtgcgccgc gagcaccgcg ccggtcgaga tccgccgcac
     7921 ctcgaagtag aagcagttgg tatcgctggt ggcggaggcc gcgcggcggt agtcgaacgt
     7981 ggcgccgctg acagccttgc cggcgggcag cgccgtgctc aggtcgaagt ccacgtagcg
     8041 agtcgccgcg aacgtgggcg cccagttgcc agtggtcgtg acgcggccgt cgccggagaa
     8101 cgccgagggg gccgactcgt ccacgagcgt ggtggtcgag acgtcgcgga ggctccacgc
     8161 cgaggtggtg atcgtggtgg tcgacgaaac ggccaccggc gcgctcgcga cgtcgcccgc
     8221 gctccccacc gcgtagatcg tgtgcgagcc gttcgacacg cccaccggca gggtcgcgct
     8281 caccgtcgcg gatccgctcg aggggatcgt ggccggcgcg acggtggatg tgagcaccgt
     8341 cccgctcgac gcgtcgtcga ggcggaccgt cacggtctgg ccgggcttga acgacgccag
     8401 gctgccgctg agggtggccg gaagcgacgt gaccgacgcc ggcgcgaggg tgaggcccgg
     8461 cgccgcgacg ctcacagcgg tgctctggcc gctctccggg ccggtccagt tgccctgctt
     8521 gggctcgacc gagtagcgcc agctaccggc cggcagatcg gtctcggtgc acgaggcggc
     8581 ggtgacggtg cccgcgcacc cggcgccgat cgtctgcacc gttcccgtgc tcgagtagcg
     8641 gcgcacgacg tagccggcga ccgcggctcc gcccgggaga gtggtggccg accagctgac
     8701 cgtcacgctc cggttcgaca ccgaggcgga cggcgtggcg ccggccggca gcgtgatcgc
     8761 gcgtgccgac tggcccccga cgccggggga ctgccacgcg gccctcgacg ctactggagc
     8821 gccgaggacc gtgagcagta cgagcgcggc tgggagcaga cgacccacac cgcgagcggt
     8881 tagccgctgg cgccgctgag gttgacgggg atcgtgaagg tcgcgccctg gcaaccgttc
     8941 acggaggcgt tcgtcatgct ggccgcaccc gacagggtcg ccgtggtctg gccgcccgcc
     9001 gcgacggcga tcgacaggcc ggtcttgtcg gtgaacgaca cgcccgtggt ggtgcacgac
     9061 gcgtggcccg agtcgggcgt gatcgcgccg ctgccggcaa cgccggtcac cgtaacgggg
     9121 tacgagttgg ggttgctgat gcggatcagg acgtcgccgg cggggccgcc cgggtagagg
     9181 gtggcaacgg tgctggcgga cacgtcgacc gtggtcagcg cctgcgcggt gccggccttc
     9241 gcgtagccgg agccggctcc ggtggtggtc cacgccgcgt agacgagtcc gacggcggca
     9301 agcgccatca ccatcgtcat gacggcgagc ttcctgctca ctcgcttgct catcggaggg
     9361 ttcctttcgg ttcgcgggca ggttcgccca cctgacgtga ctccccgcta tgggggtcac
     9421 tgttttgagg tatcggccgc ggcgctgcga gctttaggca cggaggcaca ggtgtcgata
     9481 gaggacctgt ggatcgatcg cgccgaatcg ccgcgctcgc atgcgccctg gcgctgccgc
     9541 tgagcgcggc ggcgctcgcg gccgcgcagg agccggccgc gcccgcgggc gagccagccg
     9601 cccccgcggt cgaccccccg gccgcggctc cggccccaga cgtcaccgca ccgcggctcc
     9661 tgctcggcgg ccggcgcgcg cagcacctcg ccccgcacct cgaggccgac gggagctgcg
     9721 acgagtactg cgagttcgag atatccgcgc gcgtctacgg agtgccgggc ctgcgccagg
     9781 tcgacgtgct caccccggac aaggccgggg agggcggcgc gcgcatgcac ttccacatcc
     9841 tcgtctcgcc gcgggcgcac aggctgatga acgacgcgct gcgcgacggc cgccgcgtcg
     9901 cggtcgcgct gaacgtgatc gcctacgacc tcgccgacaa ccagaccgcg ggcaggcgct
     9961 ggatcagggt ccggccgccg gcctagccgg cccgagccgc cctcagacga tcgtcggcac
    10021 cgagccgccg tcgaccgacc aggcggcgcc cgccacgtcc gaggcgcgct cggagcacag
    10081 gaacgcaatt acggacgcga tctcctcctc ggtgccgaat cgtcccagcg ggatcttcgc
    10141 gcgggcgccc tcgagcgcct cctcgcgcga gctgcccttc gcagcggcgg cctggtccgc
    10201 gagcccgccc tcgcccagcc agagcggcgt atcgaccggc cccggcgcca ccgcgttcac
    10261 gagcaccccg cgggcggcgt aggtgtccgc gaacacgcgc gagagcgaga gctgggccgc
    10321 cttgctgacc gagtaggccg cgttcgagag cgacgggcgc ttgcccgagg acgagcacac
    10381 gttcacgatc cggccccagc cggcgtccgc catccggggc gcggcggcgc gcatcagccg
    10441 cagcggggcc atcacgtgca ggtcgagctg ctcgcgccag tcgtcgtcgc tgagttcgtc
    10501 gagcccgctc atccggctcg tgcccgcgtt gttgaccacg acgtcgatcg agccgaagcg
    10561 ctccacgcac tcggtcacga tccgatcgcc cgcgtccgcg ccggtcacgt cgatggcgag
    10621 cacggcggcc gccgggtccg acgcgggggc cgagtcgcgc gcggcggagg ccgcctcgcg
    10681 cagccgctcc tcgtcgcgcg ccacgagcag cacccgcgcc ccctcgcccg ccagcgcgcg
    10741 ggccgtcgcg agcccgatcc cgctgctcgc gccggtgatc acgcacgccc tgcccgccag
    10801 tcccaggtcc atcaggtcat gatcccagca tgcgctctca caccgtctac cggaccttca
    10861 acaccgcacg ccgccgcgag ttcgtgcgga tcaccgagga cgtgcagcag gccgtcgacg
    10921 acgccggcat cgcggagggc atggcgctcg tctcggccat gcacatcacg gcgggcgtct
    10981 gggtgaacga cgacgagccc ggcatccagg ccgacgccct cgagtggctc gacaaggtcg
    11041 cgccgccgag ctggcagccg cccgcgaacg acgtggcccg cgagctgctg ccggagggcg
    11101 gcgactaccg ccaccaccgc ggcggcgagg acaacgggga cgcccacctg aagaacatgc
    11161 tcgtccacca ccaggtgatc gtccccgtga ccgccggtcg cctcgacctc ggcccgtggc
    11221 aggcgatctt ctacgccgag ttcgacgggc agagggcgaa gcggctgatc atcaaggtgc
    11281 tcggcgagta ggggccgcct acgcgctcgc gccggaggtc gcctcggcga gctcctcgtg
    11341 ctcgccctcg cagaactcct cgacgatctt cgcgttgaag gccgggatgt cgtcgggatt
    11401 gcggctcgtc gtgagcccgc ggtcgaccac gacctcctcg tcgacccact cggcgccggc
    11461 gttgcgcagg tcggcctgga tgctcgggta cgaggtgagc ttcaggccgt cgaccacgcc
    11521 cgcgtcgatc agcgtccacg gcccgtggca gatcgcgccg accggcttgc cggcctcgaa
    11581 gaacgcgcgc acgaacgcgg tggcgccctc gtcggctcgc aggaggtccg ggttgtgcac
    11641 cccgccgggg agcacgagcc cgtcgtagtc ggatgcgtcg gcgtccgcga ccgtgcgatc
    11701 gacgtcgaac ttctcgccgt ggtcgagctt ctcgaagccc tggatctggc cctcctcgag
    11761 ggagatcagc tcggtgtcgg cgccctcggc ctcaacggcc ttgcgcggct cggtgagctc
    11821 cacctgctcc acgccgtcgg cggccaggaa cgcgatcttc tttccgtcga gcttgcccat
    11881 ctcgctcgcc tccttcattc gagtacctgg agctcaggta cccggcgacg ccgcggcgac
    11941 acgcggggtg gcgggctcgc gctcgggcgc cccgccgatg gcgcgcagga ccgcccgcgc
    12001 ggtctcctcg ccggtgcgat cggcgccgtc catgtagccg ttccagacgg tggaggtctc
    12061 ggcgccggcc cagtggagcg ggccgaccgg ctcgcgcagg gcgcgcccga acatcatcca
    12121 gccaccgggc ggcatgtagc acccgtagca gccgcgggtc cactcctcgt cggcccacac
    12181 gcgctccacg aagcgctccg gccgccccgc gcgctccccg aacagccgcg cgaacccgcc
    12241 caccactgca tcgcggcgct cggcggcggg gcgggctccg tagtcgcgcg cggccgcgcc
    12301 ctcgaggaag ccgagcagca cgcccggcga cccgctcggc ggtgtgttgt cgaacgtgat
    12361 gcgggccggg ccggcctcgc tgatcccctg ccccgacagt ccctgctcgc gccagaacgg
    12421 acggtcgtag atcgccatgc acttgatcac ggtgccctgc ggcatccgct gggtgagctg
    12481 gtcgcgcagg cccgggagcg gcgggtcgta ggcgatccgg cccgcgagcg ccgggggcac
    12541 cgccacgatc gcgcgccgcc cgcgcgccac gacgccgtcc gcgtgcaccg tcacgccgcc
    12601 ggcgccgtgc tcgatccgcc gcaccggcga ttcgagcacc actgagtcgc cgagcgcctc
    12661 ggccacgcgg atcgcgatga gctgcgagcc gcccacgaag cgatcctgct gcgcgccgcc
    12721 ctcggtccca agcagcgacg ccaacccgct gcccgagcgc gtgtagaaca ggacgtggag
    12781 cagcgacacg tcggccggct cggccgccca gaccgcctcg atggcgatct ccagcatcgt
    12841 ccggccgccg cgcgtgtaga cgttgcggcg catccacgac cagaacgtct gcgagtcgag
    12901 acgctccgcg ttcggtgcct cccacggggc gtcgagcggg atccgcttcg acatgcgctc
    12961 gagcttgacc tgcgcctgga ggatgtcggc gagcacgtgg ggggcgatcc ggggcgtgtc
    13021 gccgctgtag cggcgcacgc cgccgccgaa ctccacgagg ttctcgcccg cgtggtaggt
    13081 gggataggtg tcgaccccca cggcctcggc gagcgccgcc aggcggtcct gcgtcgggcc
    13141 gatccactga ccgccgacct ccacgacctg accgtcgccc agcggctcgt tcagcgtgcg
    13201 gcccccgacg cggtcgcgcg cctcgagcac cacgaccgac gcgcccgccg ctgtcagctc
    13261 gcgtgccgcc gtcaggccgg cgagaccggc cccgactacg actacgtcag cgtcacgagc
    13321 gtccaccgga tcattgtgac agtgccgcag tggcccgctc gggaaccttg atcacgagga
    13381 tcgcgatgtc gtcgcgcggg ccggagccgt tgctcgcgcc gagcgcgagc tggagcatcc
    13441 gctcggcgat cgtgtcggcg ctctcgtagc gggcggcctc gagcacggcg gcgagctcct
    13501 cgggctcgtg gatgaggtcc ggggccgcgg cgtcggtcac gccgtcggtg tagagcacga
    13561 cgacgtcgcc cggcttgagg tcgaccgtcg catccacgag gtcggggtcg ggcacgatgc
    13621 cgagcagcgt gccgggcgtg ccgacctccc cggcgcctcc ggaggcgcgc agcaggatcg
    13681 gcagcgggtg cccgccgctg gcgaagcgca ggcacgtgcc gtcctcgcgc tggtcgaggc
    13741 aggcgtagag cacggtggag aagcggcggt cggtgcgctg gcgcagcatc gcctcattga
    13801 gcgtcccgag gatgcggctc ggcacgtcct cgctcatcgc ggtggccctg agcgtgtagc
    13861 gcgcgagcgc ggtgatcgcg gccgcctccg gccccttgcc gcacacgtcg ccgatcacgg
    13921 ccgcccagcg gtcggagccc gtgtcgaaga cgtcgtagaa gtcgccgccc acctcgtagc
    13981 cctcgccggc cgcgcggaag cgcgccgcca cctcgaggcc aggcggctgc ggcagctccg
    14041 gcggcagcag gctctcctgg agcgttcgtg cgatgtgcgt gcgctcgccg aacagccgcg
    14101 cgttgtcgac cgcgatcgcg cagcgcacgg ccagctcctg ggcaagcagc agatcgccat
    14161 cgtcgaaggc gcggccggac tcggcggaca cgaacgtgat cgcgccgagc acgcgcccac
    14221 gcgcggtcag cggggcgatc atcgccgacg tcatgccgat ctcgtcgatc agccgcatgt
    14281 gctcctcgtc gacagcggcc tcggcgagca gctcacgcgg gatcgacggg tagagctccg
    14341 actcgccggt cctcaggacc cggtacacgc cgcgctcctc gcgcggatcc ggcgggtact
    14401 tgttgcgcag ctcgatcgcc cactcgacct tctccgggtc cacgtgcgcc accaccacgg
    14461 gcctcgtggt cccgtcctcc tcgaggacgt ccaccgagca ccagtcgccg atccgcggga
    14521 cggcgagccg cgccacgttg gtgatcgtcg tgtcgtagtc aagcgaggag gagaggatcc
    14581 tcccagcctc ggagaggaag cgctgcgcct cggccgcctc gcgctcctcc gtgacgtcct
    14641 ccatcacgtt gatcgcgagc accgccgcgc cggactcgtc gaagaccggc agggccttga
    14701 cccggaccca gcgctcgctg tggccgccct tgaggcggaa gcgcgtgagg atcggctccg
    14761 gcgcggggtc gccggccagc gccttgcgcc cggggaggtc ctcgaccggg atcggggcgc
    14821 cgtcgccatc ggtgacctcg aaccggttcg cgatttcgat cgcgggagtc gcgagcagct
    14881 cctcgatcga gtcgaagccg aacgactcga ccgccgcctg gttcgcgaac acgaggtggg
    14941 ccccgcggcc ctgcgcggtc acggcgtcgg ccacgccgtc gaggatcgcc tggagctcgt
    15001 cgcgcgagcg gcgcaggtcc gactcggtgc cgctcagctc ggagtagagg tgggcgctgt
    15061 ccaccgcggt cgccacgcgc ccggccagct cctcggcgag cgcgaggtcg ccctcgtcgt
    15121 aggtctggcc ggactccgcg gccgcgacgg cgagcgcgcc gaacgtcctg ccgtgcgccg
    15181 acaggggcac caccacggcc gagcgcatgc cgagccgctc gagcatgcgc aggttctcct
    15241 cgcccgccga gatcgactcg agcagccgct cgtccacatg cgcgtagagg cgcgcctcgc
    15301 cgtcgcgcat ggtggcggac gggccctccg cgcgctcctc cttcatcggg tagcgctgga
    15361 gcagctccca cgcgaggcgc tggcgctcgg ggtcgcggtg catggccgcc acctggcgca
    15421 ccccgccctc ttcgtcgcgc atgaacacga agcaccagtc cccgagctcg tgggcgagca
    15481 gccgcgccac ctcgatcgtc atcacctcgg gctcgagcgt gcgctgcacg atgtcgctgg
    15541 cacccacgag cagggcgtag cggcgccggg cgacgcgctc gcggtggcgc tcgtgcgcga
    15601 tccaggtcgc gatggcgccg cccacgacga cggtcgcgac ccgggtcagg tgctcgccgg
    15661 gatcgaggaa catgtcgttc gggatcccga cgaccagcgc gagcgcggtc gcgtagaggg
    15721 tcacgagccc cgtggagcgc gcggatgcgc cgaacgaggc cagcagcggc ccggcgacga
    15781 tcattccggt gatcaccgcg cgctcgccga cgagcgcgtc gagcgccgcg atcagggtgg
    15841 tcacggcggc gccgatgagc acccaccgcg cctgaatgcg ctgctcgcgc gcccaggccg
    15901 tcagcggccg cataggtgct gccgcgtcgc ccccggctgc gcgctcggcc gttgccacgt
    15961 ggtctctccg ccctgccctt tcgcgagtcg ccggcccgtg cgggccgccg cgctcactct
    16021 agcgccgggg aggggctgcg gagcgcccta cgcgcagttc gcccagggcg ccgtgccgcg
    16081 ctggcggtac agcttcaggg cgaggcgatc ctggaccgat tcggcggcct ttgccgggtc
    16141 gcccgagccg cccagcgcgc gccaggtggc cacgtcgaac tggaacttgc cgcggtagcg
    16201 cccgtcgcgc gagaccgcgc gcgggttgcc gccggactcg cactccgcga tgcgcttgag
    16261 cacggccggg agcgtcacgc ggcgcgtggt ggtgccatcg gagccggacg gcgcggcgcc
    16321 gccggtcgcg tacgaggcgc ggccgatctt cacgccgaga gcgcgagcgg tggccgcccc
    16381 gacgatcccg tcggcggtca ggtggcggct gcgctggaag cgcttcaccg cggcgacggt
    16441 ctggcggccg tagtagcccg tggccgtcac gcgcaggcgg cgctgcaccg ccttgacgga
    16501 ggcgccggtg ctgccgagct tgagcgtcgg cgccgcatcg gcggactgac tgccggagcc
    16561 ggagtccgcg gcgagggccg cgccggatcc gatggtgagg gccgcgcaga ctgcgagcgc
    16621 gatagtgcga atacggaagc gtgcgttcaa gttcggtgcc tttcgtgtcc tttcggcgcc
    16681 tacgaggtga gctgacgggc tcgcgcggaa agaactcgcg ctacgccgca cgctggcggc
    16741 gattcgcccc acggaccgga ctgcccggcc caattgggtc ccccgttccc caccgccctg
    16801 agcggcggag actcggctat ggcgacttag atctaagcag accttgtcaa gaaataggtc
    16861 ggcagatcgg cctctacggt ttacccccac cccgcctcgg ctaaggagaa cgcatgaact
    16921 tcaactcgct cataaccggc ccggcacggc tcgggctgac cctcgcagcc aggggccttg
    16981 gcctcgtcag gggccgtttc gggaccaccc aggtcgacga cacgacgctc cagcgctcgg
    17041 tcgaggcgga ggtgttcgcc tcgcggcgcg cgcccaaggg ccagatcgag gtcaacgtga
    17101 ccgagggcgt cgtctggctg cgcggcgagg tcaagaccca gagcgccgtg aacgagctcg
    17161 agagccgcgc acgctcggtc gagggcgtcg cgcgcgtgga gaacctgctg cgcgtcgcga
    17221 agccggccaa gagcgcgaag ccggcggcgc gcgcgaagac ccaggcgaag acgcagaagc
    17281 gccccgcggc gaagcgcagc tcgcggccga agcccgccgc ggccacggct ccgccgccgc
    17341 aggccgagcc ggtcgccgat cccaccccgc ccccggccgc ccagcgcgag cgcaaggtca
    17401 cccggcgctt caacgccgag cggaccgcgg gctccgaggc cgagcccacc ccgcgcgacc
    17461 tctccgacgc cggcaagggc cgccagcctg ccccgctggg cgcggacgag ccggcgagcc
    17521 ccgcgaccgc cggcgccgcg gcagcggccg gcgaggcgcc cgcgaccggc tcccgcgagc
    17581 cggcgggcgt cggctccccc gcggcgccgt tcccgaccac cggcggcggc tccgggtcga
    17641 gcgagaacgg ctcgaccggc aacggcgggt cggcgtaggg tccagagatt gcgcgcgcga
    17701 gcgcgcatgg ccggcggccg gtgcgggatc cggtcgccgg cgcctaacct ctgagcatgg
    17761 ccgccgagcc ggactcagac cgcggttacc gcatcgcctt cgaggcactc gagcgcggga
    17821 cgcgcgtgca gacggccgac ggggaggagc tcggcacggt caagaaggta atgatcgtcc
    17881 acgagaagca cctgttcgac gggatcgtgg tgaagaccgg gtcgggcgac cgcttcgtgg
    17941 acgcccccga ggtggacgag atcttcgagc ggctggtcac gctcaagatc agcgccgcgg
    18001 aggcggccga gctgccgaag ccgggcgcca accccgccgc gatgaggctc agtgccggcg
    18061 acttccgccc gggcggagcg cgccggctct ggaaccgcct ctccggccgc cgctgacgct
    18121 cagcgcccgc gcgaccactc gcgcctgagc gcctcccgcc cgccgctctc ccagatcgcc
    18181 ccgtgcccgg ggatcacgcg gtcgaagtcc cagtcgagga tccgctccac gtcgcgccgc
    18241 cccgtgctcc gccggatcac ggcgcgcgcg agcggcgtcg ggcgcagccg gccgtacatc
    18301 cgcccaagcc gggccaccgc gcgcgtcgcc ggcggcgagt cggcgccgaa gttcatcgcg
    18361 aggtcgccca ggatcagcgt ccggctcgcc cggtggagga actcgatctc cggcatgagg
    18421 acgttgccgc ggaacggcgc ctggtcgagc tcgccggccc agcgcgggtc gggctcgtcg
    18481 cgcaggtcgg cggcgaagct caggtccttg cggcggcggc gcaaacccgg acccgccagc
    18541 agctccacgc cggggtaggc cgcgctgtac tgctccatcc atctgtggcc gtggagcttg
    18601 ttcggcgcgg ccacgaagcg cacctcgccg agtgcggcga gcccggcgcg gagcgcgtcg
    18661 tccagcgggg ccggcgagtg cacgaacagg ccaccggccg cgagccgcac cacgttcatg
    18721 atccgcccgg cctccacgcc aaggaagcgc agcggggcct cgtggaccca gagcccgtct
    18781 ccgatctcgc ggagcccgct cacggcgcca cgaactcccg cagcaggagc ccggcgcgca
    18841 gcggcgcctc gagctgcggg tagtgcccgg catcggcgag ggcgtgcagc tccgcgggcg
    18901 gcatccgcgc ggcgatccgc tccgccatgt gggcgccgga cacggggtcg agcatccccc
    18961 acacgaaccg gcgcggcacg tcggtccgct ccagcgcagc cgtccagcgc tcggcgtggc
    19021 ggcggcggtc ctcgatgtag cggatcagca ggtgctggat gcgcgcgccg ccgtggcggg
    19081 cgatcccgag ccagtgctgg tggagatcgg cgtcgctcgg gcggtgggcg aagacgcgcg
    19141 tgaggctcct caggaaggcg cgctcgttca ccgtccgcga gaacatgggg cccgccgtgg
    19201 ggtggagcag cagccgctgg gcgatcagcg ggcggtgcag gtcggggtag agccctccgt
    19261 tgaggaacgc gatgcgctcg atctcggcct ccagcgctcc ctccgcgcgc cgcgcgagca
    19321 gctcctggcc cacgctcacg ccgtagtcgt gcacgaccag cgcggtgcgc tcgataccgt
    19381 ggcggcgcca cagcgcctcg gtcaggtcgg cctgctcgtg gatcgagtag acatggtcgc
    19441 gcggcttgtc ggagtcgccg aagccggcaa agtcgaacat caggaggcgg cgcgcggcgg
    19501 ccagcccgtc cgcgaccggc gaccagtccc aggagcacgt gggaaagccg tgcagcagcg
    19561 tgagccaggg agcgccctcg gaacccgcga cgcgctcgaa gagccgcagc ccgccggcct
    19621 ccacgcgctc gccggccgcg aaccactcgt cgatgcgctc tgtcgcgatc actccggcgc
    19681 cattctcccg cctgctcaac attttcgggc gttctgccgt tgtaatgggc agatgcgcct
    19741 ccttctaccc ctcctcgcgg ccatcgccgt gctcgtgtcg gccggactcg cgctcgcggg
    19801 cccgtcgaac agctgcgcgc cggtgcgcgt tgccgcgatg acgtgcatcg gcaccgacag
    19861 cgccgagtgg ctgaccaccg gcgacggcga cgacaccctc gacgggcggg gcggcgccga
    19921 tcgcctgaac gcccgcggcg gcgacgacgt ggcgtacggc ggcagcggcg acgacaacgc
    19981 gatccacggc ggcaccggca acgaccgcct ctacggtcag gacggccgcg acgtggtgct
    20041 cggcgacgag gccggcgccg gcggcgcgga cctcgtgtcg ggcggctccg gcagcgaccg
    20101 cgtgttcggc atggcgggcg cggacgacgt gcgcggcgac tccggcaacg acgtggtcca
    20161 cggcggcgac ggccgcgacc tggtctcggg cggcgacggc gccgacgtgc tggacgacgc
    20221 cgctcgctcg caccaggacc ccgactcctt cagcggcggc ggcgggaacg acgagatcta
    20281 cagccgcgac ggcaagcgcg acacggtgaa gtgcggttcc gggcgcgacg tggcgaaggc
    20341 ggactcgcac gacaaggtgg cgagcgactg cgagcgggtc atccgcgtcg gctgacccgc
    20401 ggccgcttga acggcggcac ttaggggagg cgggtccggg ggtgtgtgca cttccgtccg
    20461 ctatttggac gctcttgaac acgcgaagtc acttccgtcc ctaataggga cgcttttgac
    20521 ttagggctgc gggatcgggg ggagcgcctt ggctctcttc gcgatctgag cccaggggtc
    20581 cccctccctc cccagccgat ccgggacggt ccgcaccgtg tacgacgtcg ataggagctt
    20641 cggatcgtcg agctcctccc agcgcagcgg cgtcgccacc ggcgcgtcgg gcttggcccg
    20701 caccccgtag ggcgccaccg cgtgctgggc gtaggcgttg cggcccacgt ccacgtagat
    20761 gcggtcgccg cgcttggcct tgctccagac catcgtgagc cgcttcgggt cctgctcgac
    20821 gagcagcacc ccgatcgcct cggcgaacgc gcgcacctcc tcgaaggtgc gcgtgcggcg
    20881 cagcgggacg gtcacgtgga ggccgcgcga gccggaggtc atggcgtgcg gggcgagacc
    20941 caggtcgcgc agcacgtccc cgagctggag cgccgcggcg cggacctcgg cgaagcgctc
    21001 gtgctcgggg tcgaagtcga agatcaggcg gtcgggcttc tcgatcttgt ccgcccgcga
    21061 gagccacgcg tgcggcgtca cgcagttctg cccggccagg tagacgagcg tctccgggcg
    21121 gttggccacc acatgcgaca cggttccgcc gcgcttcgcc acctcgaccc gcttgatcca
    21181 gtccgggaag tgcttcggcg cctgcttcat gtagacgccc tcggcctcga tcccgcgcgg
    21241 atacagatgc atcgtcagag ggcggtcccg gacatgggga accatcacgg agcccaccga
    21301 ctcgtaataa gccgcaagct cccccttggt gatgcccttg ccgttcttgc cggggaacat
    21361 caccttgtca gggctgctaa ctcggacgtt cacggaccac ctcccgaggg ttcttgtcct
    21421 cgcgcagccc catgtagcgc ggatggcgca gccggccgtc gcgcgtccac tccgtgaaac
    21481 cgacctgcgc gacgagccgc ggctccaccc aggtggcgct gcgcaggcgc ggcgagtcgg
    21541 cgaatggcgt gtcgtcgcgg cgcagcggag cgagacgggc ggcgaggtcg cgcagcgtct
    21601 cgcgcccgaa cccggtgccg accttgcccg cgtagcgcag ccgcccgtcc tcccagtggc
    21661 ccaccagcag cgcgccgagc tcgatccgcg accccttcgg cgccgtgtag ccaccgatca
    21721 cgagctcctg ctcggcgtcg cacttccact tgagccagtc gcgcgagcgc ttgccgacgt
    21781 acggcgagtc ggcgcgcttg gcgatcaacc cctcccaccc gcgccggcag gcctcctcgt
    21841 acagcgcggt cccggaagcg cggcggtagg gggtgaggcg gatcggaccg cccggcttca
    21901 ccaggcgccg caggaccgcc ttgcgctcca tcagcggcag ctcgcgcacg tcctccccct
    21961 cggcctcgag cacgtcgaac acgtagaaga agacctccct cccccgctcg cccaagcgct
    22021 gaaagctggt ctgcgagccg gagaacgcca ccacctcccc gtcgagaacg gcgctgggga
    22081 aggaggcgag ggctgcggcc acggtcggga agcgaccggt gaggtcgaga tcgttgcggg
    22141 agcgaaggtc cgggtgcggc cgggtcgttg ccaggcacct gatcccgtcg agcttgcgct
    22201 cgaagatcca gtccgggtcg tcgaagcgcg cgtccgtgag cgtggcggcc atcgccttca
    22261 tccccgctcc gccacctcct cgatcgtccg cccgctgacc accgactcgg gctgcgtgct
    22321 ggtcgggcgc cgccgcgcgt ccgcctcctc gtcgcgcttc ttgatgagca gccacttcgg
    22381 cttcgcgccc tcgtgggtgc gccgcagcga ccatcccccg cgcagcttct tcccgtcgag
    22441 ccagaacgac gcgtgcccgc gcgcgagcgc ctcgtgcatc ggcacgtcgt tcagattgcg
    22501 gtaggtgccg cggtcccaca cgatcaccgc gcccttgccg tagccgccgc cgatcacgcc
    22561 ctcgaagtcg ttccacgaca gcggatggtc ctccacctgc atcgcgagcc gtttgtcgcg
    22621 cgggtcggtc gacggcccct tcgggaccgc ccaggaccgc atcgccccgt ccacctcgag
    22681 ccggaagtcg tagtggagcg aggtcgcgtc gtgcttctgg atgacgtaga ccggctcagc
    22741 caagcgcgtc ggccaccagc cgcaggtcgg ccgcgaacgc ctcgcgctcg gcctggcggg
    22801 cgtcgtcgtc gggagcgcgc agtatcgccg aggggtggat cgtggccgtc acgagcggcg
    22861 ccaggtcgga ctcgaccggc cgcccgcgct gctgggtcac gcggaagctc gcgcccagga
    22921 tcgccttgcc ggcggtggcg ccgagcagca ccaccgcctc cggcgagagc agcttcagct
    22981 cctcctccag ccacggcttg caggcgtcga tctcgaa
//
LOCUS       DASVPL010000123         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_54810, whole genome shotgun sequence.
ACCESSION   DASVPL010000123 DASVPL010000000
VERSION     DASVPL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54810"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VF517_06315"
     CDS             <1..749
                     /locus_tag="VF517_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8052589.1"
                     /translation="TKTVGGWRHFKQHTVNSSLSTWMAFGSVPAAVAGVYGLHALEKA
                     TGKSFDNTVLTIVAAALMFTGVMVLARALFLPKLVAREREVLELERRHKVGAVLLGVS
                     VGFVLGVTSAGSGSLIAVGLIMLFRLAPRQVVGTDVFHAAILLWAAAIAHVVAGNVDY
                     GLAGTILLGSIPGVWIGSHWSIRTPVATLRTGLGVIMIGAALGLLSKAGAEIPVVVLG
                     GIPLLLLALLARPQIMRWLRPVPPAPAPER"
     gene            787..>2309
                     /locus_tag="VF517_06320"
     CDS             787..>2309
                     /locus_tag="VF517_06320"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX8052590.1"
                     /translation="MFSKVLVANRGEIAIRVFRALEELGIGTVAVYSEPDRDAPHVRR
                     ADEAYLVGPGPANESYLVVEKIVEAAQKAGAEAVHPGYGFLAENAAFARACEEAGIVF
                     IGPPADAIDAMGSKTRARELMKAAGVPIVPGTTDPVDTVEDAARIVDEAIGYPVAIKA
                     AGGGGGKGFRVALEAGELEKAFEGAAREGETFFSDPTVYIERYLPDPRHVEVQVLADA
                     HGNVIHLGERDCSVQRRHQKLIEESPAPAVDEQLRARIGEIGTEAARAVGYRSAGTVE
                     GLLADGEYFFLEMNTRVQVEHCVTEMTTGVDIVREQIRIAAGEELSLAQDDVRLSGHA
                     IECRINAEDASKNFAPAPGTIESYDEPAGPGVRVDSGVEAGSEITPLYDPMIAKLIVW
                     DVDRDGATRRMVRALREFELGGVRSLIPFHIGLLQTEQWARGETCRDLIEDKSWLKQF
                     AQPKPEKKDDDDEPQVERSYAVEVSGRRFDVKVIGAAVAGGGGNGAAPAAARKAPPRR
                     "
BASE COUNT          322 a          806 c          866 g          315 t
ORIGIN      
        1 tcacgaagac cgtgggcggg tggcgccact tcaagcagca cacggtcaac tcgtcgctgt
       61 ccacgtggat ggcgttcggc tcggtgcccg cggcggtcgc gggcgtctac ggcctgcacg
      121 cgctcgagaa ggcgaccggc aagtcgttcg acaacacggt gctgacgatc gtcgccgccg
      181 cgctgatgtt caccggcgtg atggtgctgg ctcgcgcgct gttcctgccc aagctggtgg
      241 cccgcgagcg cgaggtgctg gagctcgagc gccgccacaa ggtgggcgcc gtgctgctcg
      301 gcgtgagcgt cggcttcgtg ctgggcgtca cctcggcggg cagcggctcg ctgatcgccg
      361 tcggcctgat catgctgttc cggctcgcgc cgcgccaggt ggtgggcacc gacgtcttcc
      421 acgccgcgat cctgctctgg gcggccgcga tcgcccacgt ggtggcgggc aacgtggact
      481 acgggctcgc cggcacgatc ctgctcggct cgatccccgg cgtctggatc ggaagccact
      541 ggtcgatccg cacgccggtc gccacgctcc gcaccggcct cggcgtgatc atgatcggcg
      601 cggcgctggg gctcctgtcg aaggcgggcg ccgagatccc cgtcgtcgtc ctcggcggca
      661 tcccgctcct cctgctcgcg ctgctggcgc ggccgcagat catgcgctgg ctgcggcccg
      721 tgccgcccgc gcccgcgccc gagcgctgag ctgccccggg tcgagcctca gtagatttcg
      781 cggccgatgt tctccaaggt gctcgtcgcc aaccgcggcg agatagcgat tcgcgtcttc
      841 agggcgctcg aggagctcgg gatcgggacg gtcgccgtct actccgagcc cgaccgcgac
      901 gcgccgcacg tccggcgcgc ggacgaggcc tacctggtcg gcccggggcc ggccaacgag
      961 agctacctcg tggtcgagaa gatcgtcgag gccgcgcaga aggcgggcgc cgaggcggtc
     1021 cacccgggct acggcttcct ggccgagaac gccgccttcg cgcgcgcgtg cgaggaggcg
     1081 gggatcgtgt tcatcggccc gccggccgac gcgatcgacg cgatgggctc gaagacccgc
     1141 gcgcgtgagc tgatgaaggc ggccggcgtg ccgatcgtgc cgggcaccac cgacccggtc
     1201 gacaccgtcg aggacgccgc ccggatcgtg gacgaggcga tcggctaccc ggtggccatc
     1261 aaggccgcgg gcggcggcgg cggcaagggc tttcgcgtcg cgctcgaggc gggggagctc
     1321 gagaaggcct tcgagggcgc cgcgcgcgag ggcgagacgt tcttctcgga cccgaccgtc
     1381 tacatcgagc gctacctgcc cgacccgcgc cacgtggagg tgcaggtgct ggcggacgcc
     1441 cacggcaacg tgatccacct gggcgagcgc gactgctcgg tgcagcgccg ccaccagaag
     1501 ctgatcgagg agagcccggc gccggcggtc gacgagcagc tccgcgcgcg catcggcgag
     1561 atcgggaccg aggccgcgcg ggcggtcggc taccgctccg ccgggaccgt cgagggcctg
     1621 ctcgcggacg gcgagtactt cttcctagag atgaacacgc gggtgcaggt ggagcactgc
     1681 gtgaccgaga tgacgaccgg cgtagacatc gtccgcgagc agatccggat cgccgccggc
     1741 gaggagctgt cgctggcgca ggacgacgtg cggctgtccg ggcacgcgat cgagtgccgg
     1801 atcaacgccg aggacgcttc gaagaacttc gcgcccgcgc cggggaccat cgagagctac
     1861 gacgagcccg cggggcccgg cgtgcgcgtg gactccggag tcgaggccgg ctcggagatc
     1921 acgcctcttt acgacccgat gatcgccaag ctgatcgtgt gggacgtcga ccgcgacggg
     1981 gccacccgcc ggatggtccg cgcgctccgg gagttcgagc tgggcggcgt gcgctcgctg
     2041 atcccgttcc acatcgggct gctccagacc gagcagtggg cgcgcggcga gacctgccgg
     2101 gacctgatcg aggacaagtc gtggctcaag cagttcgccc agccgaagcc cgagaagaag
     2161 gacgacgacg acgagccgca ggtcgagcgg agctacgccg tcgaggtctc gggccgccgc
     2221 ttcgacgtga aggtgatcgg cgccgccgtg gccggaggcg gcggcaacgg cgcagccccc
     2281 gcagcagccc gcaaggcccc gccgcgccg
//
LOCUS       DASVPL010000124         5095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_369550, whole genome shotgun sequence.
ACCESSION   DASVPL010000124 DASVPL010000000
VERSION     DASVPL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369550"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VF517_06325"
     CDS             <1..146
                     /locus_tag="VF517_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX8052591.1"
                     /translation="GLAYRSHHHRNRALPDWLAYYNERRPHSSIGGLAPISRVHNVRG
                     QDS"
     gene            complement(143..2254)
                     /locus_tag="VF517_06330"
     CDS             complement(143..2254)
                     /locus_tag="VF517_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052592.1"
                     /translation="MPRAFVLAAVCALLCAAPAAADPAASESLALQHGLIDAGLDQTC
                     TIDAHHDLRCWGENDYGQLGLGHTKPIGDNELPSSVGPVDLGPGRSARSVTAGYSTCA
                     ILDDAAVKCWGPNGNGQLGLGHTQTIGDNETPAAASTVDLGAGRTARALSMGWSHTCA
                     LLDDGNVRCWGIGGPQLGYGNNSHVGDDETPGSKDPVNIGTGRTAKAIASGAAHTCAI
                     LDTGQVRCWGYNGDGQLGLGSTETVGDNESPASRPVVDLGPGRTAVAITAGAAHTCAI
                     LDDGSVRCWGMGTHGVMGSGSTESIGDNETPGSKPPVDLGGSRTARALVAGGYHTCAL
                     LDNGTVRCWGFNGSFELGLPGKGNIGDDESPGGESPVSLGSGRTATALAGGEYHTCAV
                     LDDGTLRCWGYGGGGQLGGGKSEVIGDDETPGSAAPVSLGGPLFARVADLSVGASFEP
                     GAVRAGDQAHLRVTLANAGPAAAVAGVAVTLPAGLEVVSAAGPGSFDAGTRRWSPGAA
                     ASGAAPVLDLTVRATKVGSFAAGAEIVSSDAADPDSIPGRGTGEDDSASASLAVAEAT
                     VGPDPKPDPKPDPKPDPQPDPDPAPRDTLAPAFAKAPKLKPARFRAGAKRGTTVSYAL
                     SEAATTTFTVERAVSRKRFKALKGAVTQAGRLGANRLRFTGVLRGRALAPGRYRLVVR
                     ARDAAGNLSEVRRVTFVVVRR"
     gene            complement(2395..>5095)
                     /gene="rpoC"
                     /locus_tag="VF517_06335"
     CDS             complement(2395..>5095)
                     /gene="rpoC"
                     /locus_tag="VF517_06335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX8052593.1"
                     /translation="GRSVIVSGPSLRLHQCGLPKLMALELFKPFIMARLVERKSVQNI
                     KAAKKMVDSMIPEVWDVLEEVIQQHPVLLNRAPTLHRLGLQAFEPVLVEGKAIQVHPL
                     VCSAFNADFDGDQMAVHLPLSAEAQAEARLLMLSANNILSPAHGAPLATPTQDMILGS
                     YYLTYGPDEHELEERRKQEQTGKWDEKKLGKRPHVFRSAMEAELSYEHGMVNLHDLAE
                     YRRRGVESHVLTTVGRIIFNDRVERALEETLEGEYDPTDYEFVNRPLKKKDMGAVIQR
                     LVDKHGPYAVALVLDAFKDVGFHFATQAGITVSKNDVLIPPQKEEILERYEAKVSEIH
                     DQYDMGLISPEERHDRVVELWTAATDEVGTAMQDNLPPLNSIFMMANSGARGSFKQIR
                     QLAGMRGLMANPKGEIIERPIKANFMEGLSVLEYFISTHGARKGLADTALRTADSGYL
                     TRRLVDVSQDVIVRAEDCGTKEFITTPLKRADGSLNSSIAGRFAAEEMKTKRGRALLE
                     KGQLITHADVDEIVEALGDEPAEVPVRSVLKCEAEAGICQACYGTAMATGKLVAIGDA
                     VGIIAAQSIGEPGTQLTLRTFHTGGVAGADITHGLPRVVELFEARKPKGLAKLAEEEG
                     VVSVEDTEKARRVIVTTAAGEENAYPFPVRTRLFVEPGDKIKVGTQLNEGSIYPAELL
                     DTRGRTDTEVYLVREVQKVYKSQGVDINDKHIEIVVRQMMKKVRIDQRGSSNYLPGQL
                     VDRHELLRENARIKKEKGEQAEYAEVILGITKASLATDSFLSAASFQETTKVLTDAAL
                     EGKTDRLLGLKENVIIGKLIPAATGLRRYRRLEIEPSEPIPRPSAEEMGLLDESELAA
                     ELGLTGNGDIDGFSFGGDGADAAGFADELADLATPPPDEPAGS"
BASE COUNT          652 a         1817 c         1812 g          814 t
ORIGIN      
        1 acgggctcgc ctaccgctcc caccaccacc gcaaccgcgc cctgccagac tggctcgcct
       61 actacaacga gcgcaggccg cacagctcga tcggagggct agcgccgatc agccgcgttc
      121 acaacgtccg tgggcaggac agctagcggc gcaccaccac gaacgtgacc cgcctgacct
      181 cggagaggtt cccggccgcg tcgcgcgcgc gcacgacgag ccggtagcgg cccggtgcga
      241 gcgccctgcc gcgcagcaca ccggtgaacc tgagcctgtt cgcgcccagc cggcccgcct
      301 gcgtgacggc gcccttgagc gccttgaagc gcttgcggct cacggcccgt tcgaccgtga
      361 aggtcgtggt cgcggcctcg gacagcgcgt aggagaccgt ggtcccgcgc ttcgcaccgg
      421 cccggaagcg cgccggcttg agcttcggcg ccttcgcgaa cgccggggcc agcgtgtcac
      481 gcggcgccgg gtccgggtct ggctgcgggt cgggcttcgg gtccggcttg gggtccggct
      541 tcggatcagg gcccacggtc gcctcggcga cggcgagcga cgccgacgcg ctgtcgtcct
      601 cgcccgtgcc gcggccgggg atcgagtccg ggtcggcggc gtccgaggac acgatctccg
      661 cgcccgcggc gaagctgccc accttcgtcg cgcgcacggt caggtccagc accggcgccg
      721 cgccggacgc cgcggcgccc gggctccagc gccgcgtgcc ggcgtcgaac gagccgggtc
      781 cggcggcgga gacgacctcc aggccggccg gcagcgtcac cgcgacgccg gcgacagccg
      841 cggccgggcc ggcgttggcg agggtgaccc tcaggtgcgc ctggtcgccg gcgcgaacgg
      901 cacccggctc gaacgacgca ccgacgctga ggtccgccac gcgggcgaac agcggcccgc
      961 cgaggctgac cggcgccgcg ctcccgggcg tctcgtcgtc gccgatcacc tccgacttgc
     1021 cgccgccgag ctggccgccg ccgccgtagc cccagcagcg cagggtgccg tcgtcgagca
     1081 cggcgcaggt gtggtactcg ccgcccgcca gagctgtggc cgtacgcccg ctgccgaggc
     1141 tcacggggct ctcgccgccc ggcgactcgt cgtcgccgat gttgcccttc ccgggcaggc
     1201 cgagctcgaa gctcccgttg aagccccagc agcgcacggt gccgttgtcg agcagcgcac
     1261 acgtgtggta gccgcctgcg acgagagccc gcgcggtgcg cgagccgccg agatcgaccg
     1321 gcggcttcga gcccggcgtc tcgttgtcgc cgatgctctc ggtgctgccc gagcccatca
     1381 cgccgtgggt gcccatcccc cagcaacgga ccgagccgtc gtcgaggatc gcgcaggtgt
     1441 gggccgcgcc ggcggtgatg gcgaccgcgg tgcggccggg gccgaggtcg acgaccggcc
     1501 tcgaggcagg cgactcgttg tcgccgacgg tctccgtcga gccgagcccg agctggccgt
     1561 cgccgttgta gccccagcag cgcacctgac ccgtgtcgag gatcgcgcac gtgtgcgccg
     1621 ccccggacgc gatcgccttc gcggtacggc cggtcccgat gttcacggga tccttcgacc
     1681 ccggcgtctc gtcgtcgccg acgtgggagt tgttgccgta gccgagctgc gggccgccga
     1741 tgccccagca gcggacgttc ccgtcgtcca agagtgcaca ggtgtggctc caacccatgc
     1801 tgagcgcgcg cgccgtgcgc cccgcgccga ggtcgacggt gctcgccgcg gcgggcgtct
     1861 cgttgtcccc gatcgtctgg gtgtggccca gtccgagctg gccgtttccg ttcgggcccc
     1921 agcacttcac ggcggcgtcg tcgaggatcg cgcaggtcga gtagccggcg gtgaccgagc
     1981 gggcgctgcg gcccgggccc aggtcgaccg gcccgacgga cgacgggagc tcgttgtcgc
     2041 cgatcggctt ggtgtggccg aggccgagct ggccgtagtc gttctcaccc cagcagcgca
     2101 ggtcgtggtg ggcgtcgatc gtgcaggtct ggtccaggcc ggcgtcgatc aggccgtgct
     2161 ggagggcgag cgactccgac gcggcggggt cggcggccgc cggcgcggcg cacagcagtg
     2221 cgcagacggc ggcgagcacg aatgcgcggg gcatccgggt catcgtgggg ctccttcggt
     2281 cgcgaggttc tgccatcgcg gccactttcg cccggatccg gcccgcctac atcgtggaga
     2341 cccccaatcc tgccccccaa tatgtcgggg ggcaggtctc taagggtgtg ggggctagct
     2401 gccggccggc tcgtccggcg gcggcgtggc caggtcggcc agctcgtcgg cgaagcccgc
     2461 cgcgtcggca ccgtcgccgc cgaagctgaa cccgtcgatg tcgccgttgc cggtcaggcc
     2521 gagctcggcc gccagctcgc tctcgtcgag caggcccatc tcctcggcgc tcggccgcgg
     2581 gatcggctcc gacggctcga tctcgagcct ccggtagcga cggaggccgg tggccgccgg
     2641 gatcagcttg ccgatgatga cgttctcctt caggccgagc agcctgtcgg tcttgccctc
     2701 gagcgccgcg tccgtgagca ccttggtggt ctcctggaac gaggcggccg agaggaagga
     2761 gtcggtcgcg agcgatgcct tcgtgatgcc gaggatgacc tccgcgtact cggcctgctc
     2821 gcccttctcc ttcttgatcc gggcgttctc ccgcaggagc tcgtgacggt cgacgagctg
     2881 ccccgggagg tagttcgacg atccgcgctg atcgatccgg accttcttca tcatctggcg
     2941 gacgacgatc tcgatgtgct tgtcgttgat gtcgacgccc tgcgacttgt agaccttctg
     3001 cacctcgcgc acgaggtaga cctcggtgtc ggtgcggccg cgggtgtcga gcagctcggc
     3061 cgggtagatc gagccctcgt tcagctgcgt gccgaccttg atcttgtcgc ccggctccac
     3121 gaacagccgc gtgcggaccg ggaacgggta ggcgttctcc tcaccggcgg ccgtcgtgac
     3181 gatcacgcgg cgggccttct cggtgtcctc caccgagacg acgccctcct cctcggcgag
     3241 cttcgcgagg cccttcggct tgcgcgcctc gaacagctcg acgacacgcg ggaggccgtg
     3301 ggtgatgtcc gcgccggcga cgccgccggt gtggaacgta cgcagcgtga gctgcgtgcc
     3361 cggctcgccg atcgactggg cggcgatgat gcccaccgcg tcgccgatcg ccacgagctt
     3421 gccggtggcc atcgccgtgc cgtagcacgc ctggcagatg ccggcctcgg cctcgcactt
     3481 gaggaccgag cggaccggga cctccgcggg ctcgtcgccg agcgcctcga cgatctcgtc
     3541 cacgtcggcg tgcgtgatga gctgaccctt ctcgagcagg gcgcgcccgc gcttggtctt
     3601 catctcctcc gcggcgaagc gaccggcgat gctcgagttg agcgacccgt cggcccgctt
     3661 gagcggggtg gtgatgaact ccttggtgcc acagtcctcg gctcggacga tcacgtcctg
     3721 tgagacgtcc acgaggcggc gggtcaggta gcccgagtcc gccgtgcgga gcgcggtgtc
     3781 cgcgaggccc ttcctggcgc cgtgggtcga gatgaagtac tcgagcaccg acaggccctc
     3841 catgaagttc gccttaatcg ggcgctcgat gatctcgccc ttcgggttgg ccatcaggcc
     3901 tcgcatcccg gcgagctgac ggatctgctt gaacgaaccg cgggcgcccg agttggccat
     3961 catgaagatg gagttcagcg ggggcaggtt gtcctgcatc gcggtgccga cctcgtcggt
     4021 ggccgccgtc cacagctcga ccacccggtc gtgccgctcc tcaggcgaga tcaggcccat
     4081 gtcgtactgg tcgtggatct ccgagacctt ggcctcgtag cgctcgagga tctcctcctt
     4141 ctgcggcggg atgagcacgt cgttcttcga gaccgtgatg cccgcctggg tggcgaagtg
     4201 gaagcccacg tccttgaacg cgtcgagcac cagcgcgacc gcgtacgggc cgtgcttgtc
     4261 gacgagccgc tggatcacgg cacccatgtc cttcttcttc agcggacggt tgacgaactc
     4321 gtagtcggtc gggtcgtact cgccctcgag cgtctcctcc agcgcccgct cgacgcggtc
     4381 gttgaagatg atccgcccga ccgtcgtgag cacgtggctc tcgacgccgc ggcggcggta
     4441 ctcggcgagg tcgtgcaggt tgaccatgcc gtgctcgtag gagagctcgg cctccatcgc
     4501 ggagcggaag acgtgcgggc gcttgcccag cttcttctcg tcccacttgc cggtctgctc
     4561 ctgcttgcgg cgctcctcga gctcgtgctc gtcgggcccg taggtcaggt agtagctgcc
     4621 caggatcatg tcctgggtcg gcgtggccag cggcgcgccg tgcgccggcg acaggatgtt
     4681 gttcgccgac agcatcagca gccgggcctc ggcctgggcc tcggccgaca gcggcaggtg
     4741 caccgccatc tggtcgccgt cgaagtccgc gttgaaggcg gagcagacga gcgggtggac
     4801 ctggatggcc ttgccctcga cgagcacggg ctcgaatgcc tggagaccca ggcggtggag
     4861 cgtcggcgcg cggttgagca ggaccgggtg ctgctggatg acctcctcga gcacgtccca
     4921 gacctcgggg atcatcgagt cgaccatctt cttggccgcc ttgatgttct ggacggactt
     4981 gcgctcgacc agccgggcca tgatgaacgg cttgaacagc tcgagggcca tcagcttcgg
     5041 cagaccacac tggtgcagcc gcagcgacgg acccgacacg atcaccgagc ggccc
//
LOCUS       DASVPL010000125        30366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_289622, whole genome shotgun sequence.
ACCESSION   DASVPL010000125 DASVPL010000000
VERSION     DASVPL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 30366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30366
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289622"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VF517_06340"
     CDS             complement(<1..868)
                     /locus_tag="VF517_06340"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX8052594.1"
                     /translation="MSIELCGVRLRGPVLNGSGTFDAIAARRAFGDAVLDEFPFDAFV
                     SKTITLAPRQGNAPPRLWESPAGLINSIGLPNKGLAGFLREDLPKLARLPVPLVVSVM
                     GFSRDELAMLVKTVGARDEVAMIELNVSCPNVETGLIMGADPTETAAAVDRLRPLTRK
                     PLIVKLTPNATDPAAVAVAAESAGADALSLINTLKGMAIDRRTRTPWLGAGTGGVSGP
                     AVRPIALAQVAQVAAAVSIPIVGMGGIASGRDARDFLDAGATAIAVGTESFRDPAAGT
                     RIARELAALSPAP"
     gene            complement(942..1979)
                     /locus_tag="VF517_06345"
     CDS             complement(942..1979)
                     /locus_tag="VF517_06345"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052595.1"
                     /translation="MSAANGSASRTLAPFGRRSAAVVESRALGGYRLLTVLDAAGPRP
                     DPGQFYMLSTAERWGSDGERPYLPRAFSHARVREGDGGSGETDGGSAEAGAGLRLDFL
                     LEDVGPGTERLARTAAGEEVRLVGPLGIGFAAPGGSPPGSATLSGSAAPGSAAPGGSA
                     ATGSAARSGFAAPVSAPSGFAAPVSAPSALLVGGGIGAAPILALSDELGPSAPVMLGF
                     RNSDYAEAASLFDAEPALATDDGSAGRHALVTELLREALDEAAAAPGSAPPTVFACGP
                     PPMLEAVRALCAERDVPAQLAMESGMACGFGACFGCVVPTRDGYVRLCVDGPVLDASE
                     LETALVAGAGH"
     gene            complement(1976..5104)
                     /gene="carB"
                     /locus_tag="VF517_06350"
     CDS             complement(1976..5104)
                     /gene="carB"
                     /locus_tag="VF517_06350"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX8052596.1"
                     /translation="MPRRDDIKKILVLGSGPIVIGQAAEFDYSGAQACKVLLEEGYEV
                     VLVNSNPATIMTDPEFATATYIEPLLPESVAAVIEREKPDALLPTLGGGTALNLARAL
                     WEDGTLGRHRVELIGANYEAIRRAEDRLLFRQTMEAAGLRVPRSTVVTSMAEGERALA
                     KVGLPAIMRPGFTMGGHGGGTAFTESEYRERVAEGLAASPIGQVLVEESVIGWGEYEL
                     ELMRDRNDNVVIVCSIENIDPMGVHTGDSVTVAPQQTLSDVQYQALRDQAITVIRAVG
                     VETGGSNIQFAVNPETEEIVVIEMNPRVSRSSALASKATGFPIAKIAARLAVGYALEE
                     IDNDITQRTPASFEPTIDYVVVKWPRFAFEKFPGADGLLGTHMQSVGEAMAIGRTFKQ
                     AFAKAKRSRELDVLPTAPTDTDELLTRLERPAHDRYELLDEAFRRGVSADELHARTHI
                     DPWFLAEIGALARGENPETGLARTFKSVDTCAAEFEAETPYYYSGWERPGADGPRHEI
                     RRGERDAVMILGAGPNRIGQGIEFDYCCVHAAMTVRASGRDAVMVNCNPETVSTDYDT
                     SDRLYFEPLTLEDVLGIVEVERPEGVIVQFGGQTPLKLADGLKAAGVPILGTPVESID
                     LAEERTRFISLLDELGLRGPPWAAAEGPEAALAIAGDIGYPLIVRPSYVLGGRAMEIC
                     YSGEELADYLRRTGPGPDAVIYLDRFLENAIEIDVDALCDGETMAIGGIMQHVEEAGV
                     HSGDSACVLPAMSLGEDMIRRIESATEKIALRLGVVGLINIQFAVHGDDELYVIEANP
                     RASRTVPFVSKAVGVPLAKIATRLILGERLADLDAELEPARRSRREHIAVKEAVLPFN
                     RFARADSLLGPEMRSTGEVMGVARDYPAAFGKAQAAAGASLPVTGTVFISVTDGDKPA
                     ATQLAAGLHDLGFKILATGGTAQAIRRMGVPVERINKVNEGGPGHVVDRIESGSVDLV
                     INTPTGAGARADGWEIRRAAVSRGIPCITTMTGASAAQRAIRAGRGEAPEVRSLQELH
                     AVGAPEPREART"
     gene            complement(5097..6224)
                     /gene="carA"
                     /locus_tag="VF517_06355"
     CDS             complement(5097..6224)
                     /gene="carA"
                     /locus_tag="VF517_06355"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX8052597.1"
                     /translation="MSGYILLEDGTRFDGDMVGAPEASTGEVVFNTAMSGYQESVTDP
                     SYRGQIIVFTYPHIGNYGVSQAAMESDAIHARAVVMREGVDREDAPSAELGWLTWLAD
                     CGIPALSGIDTRALVRHIRDKGAMRGGVFPASLAESQARERVMAEPPMDGRDLACEVT
                     PSEPIVLDPDNPGPLVVGIDTGIKGSIVRNLRQRGVRLELHPCGTSAEELLARRPDAV
                     FLANGPGDPAAMDHVVANVRELVGKVPVWGICMGHQLLCRAVGLETFKLPFGHRGANH
                     PVKDLATGRIDITSQNHGFAVQGPAGEGTIDTDEPIRWETDFGAAELAQLNLYDRTVE
                     GLVLRDVPASTVQYHPEAGPGPHDSLYLFDRFLDGLATANA"
     gene            complement(6221..7567)
                     /locus_tag="VF517_06360"
     CDS             complement(6221..7567)
                     /locus_tag="VF517_06360"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX8052598.1"
                     /translation="MSAPQLDFAAVPGGDVLVREVRLLDPRTDLDRPGDLLIRDGAIA
                     ELGAPGALDAPEGAEVVDGEGLVALPAFVDPHVHLRTPGREDEEDIATGSRAGAAGGY
                     CAILAMPNTDPVVDSAPVLSSLRERAREEARIPTGFTAAITRGQDGAQLTEMAELADA
                     GAFAFTDDGRPVQSAGVLRQALQYQRLAGRVLALHEEDPTLSGDGAMHEGAVSALLGL
                     SGIPSISESTAIARDAAIAGYEGGRIQVQHVSARESVAAIEAAKAAGVEITAEATPHH
                     LCLTDEAVRSLDANFKMNPPLRAEADRQALIAALRSGTLDCVATDHAPHAREEKEQPF
                     EVAPMGVTGLETAFAALHTRLVLTGLIDLALLVERMTAGGIPFGIPAPTLAPGSPANV
                     CLVDLESEWEVGEHGYESRSANCAFAGQRLTGRVRMTVAGGQVAYRERSFAIGAVA"
     gene            complement(7564..8526)
                     /locus_tag="VF517_06365"
     CDS             complement(7564..8526)
                     /locus_tag="VF517_06365"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX8052599.1"
                     /translation="MRKDLISIDALTREDIERILDRAESFAEVSGREIKKVPTLRGRT
                     VVNLFYESSTRTSSSFELAAKRLSADLVNIKSVGSSVDKGESLKDTVQTLSAYDPAAI
                     VIRAPHAGAAQLVARWSAASVVNAGDGKHEHPTQALLDVYTLRRRLGGLEGLRIWIVG
                     DVLHSRVARSDILAFGRMGCEVTVCGPPTLIPREVESLGCEVRWSLDGIGEADVIYAL
                     RMQNERMKGSFVPSLREYAARYQIDGRRLAPSQLLMHPGPVNRGVELAGEVVDSPQAL
                     IVQQVESGVVVRMAVLYELLAGGGGGSSAARFARPGATTEPQPA"
     gene            complement(8523..9080)
                     /gene="pyrR"
                     /locus_tag="VF517_06370"
     CDS             complement(8523..9080)
                     /gene="pyrR"
                     /locus_tag="VF517_06370"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX8052600.1"
                     /translation="MNEEKVVLDAEDIRRTLVRISHEVVEKNSGEPVAVVGIHRRGAI
                     LAGRVHELLCELTGDDVPLGDVDISFYRDDLATRAPDRQPVVHASHIDFPVDGSTIVL
                     VDDVLYTGRTVRAAIDAVFDYGRPERVQLAVLTDRGHRELPIRPDYVGKNLPTARNER
                     VNVRLEELDGVDEVTIATQGAEVLA"
     gene            complement(9227..10183)
                     /locus_tag="VF517_06375"
     CDS             complement(9227..10183)
                     /locus_tag="VF517_06375"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX8052601.1"
                     /translation="MDLDVLFVGTAGSAPTAGRGLTSTLIRRGGERLLFDCGEGTQRQ
                     LLRSTGLIELEEIFITHFHADHILGLPGIIKTFGLRGRERPLTVYGPTGLRALFRALA
                     PLIGRAAFELRLVELEPNEELVRDGYRIAAFDMTHRVRAYGFALVEDERLGRFDERRA
                     LELGVKPGPDFGRLHRGEAVPGSDGEVGPEQVVGESRPGRKVVLAGDGAPSPLTVAVS
                     HGADLLVHEATFSDEEVERAAETAHSTARQAAELAREAEVKLLALNHVSPRHPPGVLL
                     EEATAVFERTILPRDFDRVEVPFPERGEPKHVRREDSGQRQQ"
     gene            complement(10193..10681)
                     /locus_tag="VF517_06380"
     CDS             complement(10193..10681)
                     /locus_tag="VF517_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8052602.1"
                     /translation="MLVAIVSDTHLPRGTRRIPDDCVERMRAADLVIHAGDISTPEAL
                     AEIEALGPPVRAVHGNVDSEELRRRLPESLVVDAGPARIAVLHDAGPSKRRLERMRLR
                     FPEADAVVFGHSHIPLHEERDGFQIFNPGSPTDRRRQPRHTMGIARVARGRVAFEHVR
                     LD"
     gene            10709..10781
                     /locus_tag="VF517_06385"
     tRNA            10709..10781
                     /locus_tag="VF517_06385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10742..10744,aa:Arg,seq:ccg)
     gene            10905..13061
                     /locus_tag="VF517_06390"
     CDS             10905..13061
                     /locus_tag="VF517_06390"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMG-box domain-containing protein"
                     /protein_id="HEX8052603.1"
                     /translation="MDLPTRRCILRSIAVAADLMLLLAPPAHALDGPHNFRNANIADR
                     AEQRANGTWAGQCLVFASDMIVEAGGPRFWFGDNTSTYQAQWAQRAASVGSLAEARRG
                     DVIQWGGGAGGPGLHTAIVTAPGSNPSVIDSNVGWDERVHRGSFSSRNGSATPYRIWR
                     VGRDDSPPPPPPTDAGKPFGNVDVLTGASGGHVQVQGWTIDPDERTASTDVHVYIDGP
                     AGSPGVRGVNLGAADISRPDVAQVHGGSGERHGFGAAIPGVGPGNHSVYVYAINRAGG
                     GGNPLIGSTTVNVPADAAGSPFGAYDELLERAGYKADVHGWTIDPDAPGAATEVHAYV
                     DGPAGVGRGINLGDADVSRPDVDAVHHAGAAHGLDERLSDLAAGAHTVWLYAINRAGS
                     GDNILLGAKRVWISGPSPIGHLDVLAGALPGSIALNGWTMDPDAPTSPTEVHVYIDGP
                     AGSSGARGVNLGAADVLRPDVADVYAGGGPDHGFSTAIQGVPAGPHAVYVYAINRAGG
                     GDNPMLGSTTVNIPEKPAGTPFGSFDTASGGAGLASITGWTVDPDAPTLPTDMHTYVD
                     GPAGSGARGIDFGAADRARPDVGAANRGVGDSHGFEKTVGDLAPGRHVLWVYAINRAG
                     DGGNPLLRTLPVTVGQPAAAAPQTGEGTVIPPSGQPSLDTSACATAERAKAKAWKKMS
                     SAKRAYRKQRTAKRRRQYEKTRRAYSTAKKRATKLC"
     gene            13126..14691
                     /locus_tag="VF517_06395"
     CDS             13126..14691
                     /locus_tag="VF517_06395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8052604.1"
                     /translation="MRHWRAVPGAVVIAFLLAAAPASAAKQCSEPERDWQRATPAEAG
                     LDAAKLQDAIDYATTQSSYAVRVYRHGCLVGEDRTAAASDNLTFESWSMGKSVTSLIF
                     GRAMSLGYISPDDPVGSLVPEADAAHGSITMRNLLTMTSGLHWNGFRDYNLFTMPDRV
                     HDALTLPIDHPAGTYFEYAQSPVALIAEAIGRAVDEDVQSFAQRELMDPLGIAASAWY
                     WERDPSGHVQGFTGVNMRPGDFARLGELMRRKGMWRGRRLLAKRYVQESVAPTKTNGC
                     YGFLHWVNAGAPCVGPTITSRPVEEGRDFPDLPADMFQFSGLFGQRVTNFPSLGIVFV
                     RTGQDPSLLPAGNAEWEHDTYVRLLAAVTDQKVEQPGAPSQGPPQQEADYGFQNAIQH
                     PEDYGKGTVLFQDPLPPAGPERARSPIPLQDETRASRTGATTILMLCPGNWPGRPAPR
                     CTGTAKLAGTQRSLAYDTRAGTNLKLRFVLKDKTLRSLRRTRRMSARVTTATAAPGGA
                     TPGAVTVVVRAPH"
     gene            14767..14997
                     /locus_tag="VF517_06400"
     CDS             14767..14997
                     /locus_tag="VF517_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052605.1"
                     /translation="MGTDETGQVTGTKDKDYNVIWFTEQCLSNVLRLEQYVQDAERDG
                     DSELAEFFRRAQETSRKGAEQGKEQLKKRLAG"
     gene            15040..17280
                     /locus_tag="VF517_06405"
     CDS             15040..17280
                     /locus_tag="VF517_06405"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF010081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX8052606.1"
                     /translation="MSALAEHSYTTRGGIAVRRRTEPVGIEAAVESIIDRLDSRLGVL
                     LASRYEYPGRYTRWDIGFADPPLMVTSRGRSVRVEALNERGRVLLAPVRGAIDALEAV
                     EDLVDDDSGFTARIATPTRRFAEEERSRQPSVFSALRAIIELFASPEDPHHGLYGAFG
                     YDLAFQFEPVEQHLPRTADQRDLVLFVPDELVVVDHNRHLAERFSYDFEAGGASTEGL
                     PREGRNEPYRPTTEPPPVRCDHEPGQFADLVRKAQEAFRRGDLFEVVPGQTFYEPCPD
                     PPSEIFRRLRERNPAPFGALMNLGEQEYLIGASPEMYVRVEGRRVETCPISGTIARGD
                     DVLGDADQILELLNSAKDTAELTMCTDVDRNDKSRICEPGSVRILGRRQIEVYSKVIH
                     TVDHVEGYLRDGFDALDAFLTHAWAVTVTGAPKVWAMRFIEENERSTRRFYGGAIGVV
                     GFAGGMNTGLTLRTARVKDGVARVRAGATLLIDSDPDEEERETELKAAAFIDALRRPR
                     GVDETPVRSADLDPQAGAGRRILLVDYEDSFVHTLANYLRQTGAEVTTVRGADDDQLR
                     DALDAVGPDLVFLSPGPGAPGDFDVSRAIDLALDRELPVFGVCLGLQALVEHFGGSLG
                     LLDYPMHGKSSVIDVRGGWTFEGLPKRFEAGRYHSIYAARDALPAELTITAETDDGVI
                     MGVEHTELPVAAVQFHPESLMTTRRDVGLRLIENVVRRLAARRPAPAAAAAPPQAAAP
                     RPARAE"
     gene            complement(17207..17734)
                     /locus_tag="VF517_06410"
     CDS             complement(17207..17734)
                     /locus_tag="VF517_06410"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HEX8052607.1"
                     /translation="MAPPELQIRGGDATVKVRNPVTSALLDLVTFGIYGIYWYFVTNR
                     DLAALGRARGTKDLGDNPTNSLLALLPGAFVVIPFVISSLNTGKRIGAAQRISGIGES
                     INAVMACVAMVIFFPVGLFYAQTELNKVWERETEGGAGGGGSLAAGPGASTPLGQDAA
                     QQPAEAQLPPQAPGA"
     gene            17775..17999
                     /locus_tag="VF517_06415"
     CDS             17775..17999
                     /locus_tag="VF517_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX8052608.1"
                     /translation="MPDEVAGCEECLKTGDRWVHLRACLTCGKVGCCDSSPNRHARKH
                     AHEEDHPVIASAQPGEHWGWCFVDEVMLSV"
     gene            complement(18024..19031)
                     /locus_tag="VF517_06420"
     CDS             complement(18024..19031)
                     /locus_tag="VF517_06420"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8052609.1"
                     /translation="MSGRWDGVPVLVTGAQGFVGSWLAERLLAEGARVVVPRRAAAAE
                     SRFRRAGIEERCEVVELDIGDHDSVLLALREHRVRAVFHLASQSIADVAARSPYPTWE
                     ANVRGTYTVIEACRAAGEGVERIVVASSLHAYGPAAGARPFREDDPLRPVHPYAVSKA
                     AADSIARSYAATHGLPVAVLRMANVYGGGDLSYSRLVPDACRALVRGERPVLRSDGTP
                     ERELLYVEDAVEAYLAAAAALDDHAHAGRAWNAGSGEVAPVIDVVRRLVRASGRDLEP
                     DVRGAAAAGADRQALDSSAIRTELGWAPAWDLDRGLAATYEWYRAQLSGDPAGACGVA
                     S"
     gene            complement(19028..20221)
                     /locus_tag="VF517_06425"
     CDS             complement(19028..20221)
                     /locus_tag="VF517_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX8052610.1"
                     /translation="MSAPATRLSLRGRRLLWLTIAAGTLARLLVAILADTNTEDLASF
                     AIVRDALKSDALDFYSAVPDLRWPYPPGYLPAVAGFAGVASATGIDFLHLLRLGPIAT
                     DVGIALLVQHLLGMRGASEHIRLAAAASVALGPVFVGVAGYQGQIDSVAILPALAAFV
                     VWERGGPRRAVHAGLLLGLGGSVKTVPLLLVLALLPSVRSPREAATLLAAAAAVPLAL
                     IAPFLAHDPGAVWDHLTYRGFPGLGGLSLLVQPDLALFWQAGHDYSPNAATDALIDGG
                     GVVVAAGLIATAALLARTRPEPLGAATIVWLAVWLIGVNFFIQYLIWGLPFLLARGEL
                     RAAAAIQALAGPALLLLYLDVSSEAAIWAVYTVPMLALWALWAALLAQRVRRYSAAMP
                     GATPA"
     gene            complement(20218..20631)
                     /locus_tag="VF517_06430"
     CDS             complement(20218..20631)
                     /locus_tag="VF517_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8052611.1"
                     /translation="MPTRSIVRAGVAVLSALGVAAGVVTFRSEKRVEDAFSAAIEQRP
                     VEEVERLFDDSRPLNPGAARELAMARTNYERGRPERAEELLADAAELEPKNLRVWHLR
                     ARLARARGDEAAASRYWARARALDPHLPAALPPPL"
     gene            20645..22090
                     /locus_tag="VF517_06435"
     CDS             20645..22090
                     /locus_tag="VF517_06435"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8052612.1"
                     /translation="MAGAALLAGPVLLAFDSGGTGVSTQAAGAVLAFGLLVVLALLAS
                     WPLVERSWPLYALGALAALCAWTALSASWSPALGDAVNDADRVGFYVAAFALAVVVMR
                     APAVRRVTPDALMWGVLVVALYALAGRLLPDVVEVKRAPVAGDRLDQPLTYWNAMGIL
                     MGFGVLLATAVAADERRPLAYRAAACAAAVPCALACYLSFSRASWAAVVAGVAVLLLV
                     RPRLAPALAAWLWGAAVVVLALALRAFPAALHLDRGADAQASDGLPVALLCVGLAVAV
                     GVAFALVVRRREAAVRSPAPATSGPPPGPTFARPHGWLAAAAVAVVLGAGLAVSFATE
                     RTEDISKSAARVTTLKTYRGEYWRVALRSFADHPVGGVGTAGFQVEWIREREERVFAF
                     DAHSLYIETLAELGIVGFALLAAFAACVAGGLRRHFRAAPGDPLLAAGAAVLGAFAVH
                     AGMDWDWEMPAVTLPALLLAAACVQRPAPSG"
     gene            22087..23277
                     /locus_tag="VF517_06440"
     CDS             22087..23277
                     /locus_tag="VF517_06440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8052613.1"
                     /translation="MRILAIGSQYPPHHLGGYELVWESGMELLRDAGHEPLVLTTDFA
                     VADPEPRDDPGFPVRRDLRWYWSDHGFPRLGARERLSLERHNRRVLARALEEHRPDAV
                     SWWAMGGMSLSLVEQVRRAGIPSVAALCDDWWSYAPAVDAWTRAFATRPRLGRLAQGL
                     TGIPTRPDPAAAIGTWVYLSEELRTRAAAAGVEPLHSRVATRGPDPIFERPAEDRPWR
                     WQLIYVGRIDERKGVDLAVEAMALLPEEARLEVIGDGDAAELARLRAAAPAGRVRFER
                     VPRGELPDRVAAADALLFPVRWREPWGLVPLEAMAAGTPVIATGRGGSGEYLRHEENC
                     LIFDPDGGPPALAAQIERLASDPGLRARLRAGGAATAAAHDPRGFERAVAEELERAAA
                     KRSP"
     gene            23274..23534
                     /locus_tag="VF517_06445"
     CDS             23274..23534
                     /locus_tag="VF517_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX8052614.1"
                     /translation="MSDERPAYVYLLRCRDGSLYCGWTYDVEKRLAKHQAGTASRYTK
                     TRRPLDLAAAFELPDATAARREEARIKRLPRREKLALVPASS"
     gene            23567..24568
                     /locus_tag="VF517_06450"
     CDS             23567..24568
                     /locus_tag="VF517_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8052615.1"
                     /translation="MPTRPRLARWIPILIGTALALALVGWAVFELIPDRFASTEGLTP
                     DGRERARNDVRTSALQLLAGMVVVAGAVLSALTLRTNREGQITERFTRAIDQLGSGNL
                     PVRLGGIYALERIARDSQRDHGPIMEVLTAFVRENAPADLEQAADDARIRPPADVAAI
                     LTVIGRRIRAHETGGAPLNLARTDLRGARLDGADLRDVILSYADLRHADLVRARLEGA
                     MLVRARLERASFGVDRTMPEYGARLDEAFLMRAHLAGAHLEGAHLRRAELSNAGLMGA
                     NLAGADLDGASLGGADLSGATVGGASFRGAHFASTTVWPEGFDPEAAGALSLDLPDA"
     gene            24588..25265
                     /locus_tag="VF517_06455"
                     /pseudo
     CDS             24588..25265
                     /locus_tag="VF517_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(25252..26394)
                     /locus_tag="VF517_06460"
     CDS             complement(25252..26394)
                     /locus_tag="VF517_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052616.1"
                     /translation="MASPLRLCLVALVACLVAAVPAAGAERRAPFGFQGVDYDREASW
                     GPAGVQDEMWRAMAANGVESARVLFDWSEAQPTAGGAVSFHETDPLVARAALRGIDLL
                     PVVIYTPRWARIVSGEKHSAPADPKQYTAYLTTLIGRYGEAGTFWAEHPELPRRPLRT
                     WQIWNEPDMEYQWVPRDDWQARYGKLLRDARDAIRAADPGARVVLAGLTNSSWISLRS
                     LYKLGGIRGAFDVAALNAYTREPKNLVEIVRRGRKEMQKRGDGKKPIRVTEFGASASE
                     GRIKVGRDRDHLQTTDRKLAKLVLNAYDALAANRRKLRIEAAYWYTWASSYRSDGDIF
                     DFSGLVRYAEGSNEFAAREALPAYRKSARRLEGCAKGESSACVSRR"
     gene            complement(26440..28497)
                     /locus_tag="VF517_06465"
     CDS             complement(26440..28497)
                     /locus_tag="VF517_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX8052617.1"
                     /translation="MPFADYLAGLAFLAVTAGAALAAGLVLQRRRLAHLDGVTRVLAV
                     AVLATAALSASHLLPGLVGLLSRWSAAAVAVALAVGATRLRAAPIEWFEDPREPGPRS
                     GESPVSWAIGFAAASALALWLVASAWNRTVLPPEGIDSLTFHLPNVAGWIRSGTVWRV
                     DQFNPLLANGNYPQTGDLTFLAVVQPWRNDWLVGIVNPIYIALAGLAVYAIAVELGAP
                     RPASMLAGSLLATIPVVGLAANGEAMTDSAMFATMGVGILFLLRHARGAPRSELLLAA
                     LSLGLAFGTKWYALWAVAAVLGVWLVAARPRPLRDAVPVAAIVALAGGFWLVRNLVLS
                     GNPVFPLELSALGVTIFDAPRDVIRECAGFTIAGYLGDWDVWSKYIVPAYRETFAGPG
                     AVIGLGLVGAVVVAVRQRGALVAGLACVVLLAIGYAVTPYSAFGPEGEPLLVGANARY
                     LVPALLVAAPLAAVALGRAGRLRVPLELLALAAVVQGIDRSFDVGLGVVAAVPAVLLG
                     VALYVWWPVAEATEGWGRRSRRVARAAAAVAFAAVLIAVGNARQREFNDGRYEDGDPA
                     IAWIARHAPDGQRVALAGVWSVEGRAPVWPAFGERLRNEVDYLGPTVDEQLREYSRRD
                     RWAAAIRRGHYDLLLVGRGGYAREECPVPGQYSDDDRWAREEGFAVLARSDRLTLYRI
                     GHE"
     gene            complement(28528..28803)
                     /locus_tag="VF517_06470"
     CDS             complement(28528..28803)
                     /locus_tag="VF517_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX8052618.1"
                     /translation="MSITVKIPTQLRAVTGGEAEASVDDARTVGEVLDGLYDRYDGLR
                     DRIAEDGDLRRFVNVYVAGEDIRFLDGLETPVEDGDEVTILPAVAGG"
     gene            complement(28816..30054)
                     /gene="thrC"
                     /locus_tag="VF517_06475"
     CDS             complement(28816..30054)
                     /gene="thrC"
                     /locus_tag="VF517_06475"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX8052619.1"
                     /translation="MPAQALKCKECEQRYPLEALFVCDRCFGPLEVEYDLSALDPAET
                     RRRVQAGPQSIWRYADFLPFERPPKSALGAGSTPLVKADRLAERLGLDEVWVKNDAAN
                     PTHSFKDRVVTVALAKAQELGYEVVACASTGNLANSVAAHAAAAGLESYVFVPADLEE
                     QKILATGVYGTNLVAVRGSYDDVNRLCTELAGDRDWAFVNVNMRPYYSEGSKTLAFEI
                     AEQLGWELPSRVVCPVASGSLFTKIARGFEEWIEVGLVEGELPAFNGAQAEGCSPVAQ
                     AYEAGKDVCRPVRTPDTIAKSLAIGNPADGIYALDLARRSGGGIDSVTDDEIRSGIRL
                     LAETTGIFTETAGGVTTAVLRKLAERGDIARGERVVLVITGEGLKTLDAVRGTFEVNE
                     IEPTPRSFEEAFAAPVGAGA"
     regulatory      complement(30121..30236)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         4099 a        11116 c        11187 g         3964 t
ORIGIN      
        1 ccggcgccgg gctgagggcc gccagctcgc gggcaatgcg ggtgccggcg gccgggtcgc
       61 ggaagctctc ggtgccgacg gcgatcgcgg tcgcgcccgc gtcgaggaag tctcgcgcgt
      121 cgcggccgga ggcgatcccg cccatcccga cgatcgggat cgacacggct gccgccacct
      181 gcgccacctg cgccagggcg atcggacgca ccgcgggacc ggagacgccg ccggtgccgg
      241 cgccgagcca cggcgtgcgc gtccggcggt cgatcgccat gcccttgagc gtgttgatga
      301 gcgagagcgc gtcggcgccg gcggactcgg ccgcgacggc gaccgcggcc gggtcggtcg
      361 cgttcggcgt gagcttgacg atcagcggct tgcgcgtgag cgggcgcagg cggtcgacgg
      421 cggccgcggt ctcggtcggg tcggcgccca tgatcaggcc ggtctcgacg ttcgggcacg
      481 agacgttcag ctcgatcatc gcgacctcgt cgcgcgcgcc gacggtcttc accagcatcg
      541 ccagctcgtc gcggctgaag cccatcaccg atacgaccag cggaaccggg agccgggcga
      601 gcttcgggag gtcctcgcgc aggaagccgg ccaggccctt gttcgggagg ccgatcgagt
      661 tgatcaagcc ggccggcgac tcccacagcc gcggcggcgc gttgccctgg cgcggggcga
      721 gcgtgatcgt cttcgagacg aacgcgtcga aggggaactc gtcgaggacg gcgtcgccga
      781 aggcgcgccg ggccgcgatc gcgtcgaacg tgccggagcc gttgaggacg ggtccgcgga
      841 gccggacgcc gcagagctcg atgctcatcg gagcggcgtc cccatccggg agccgggtgc
      901 gaggggcgcc ggcctgaagc cggatgcgag gggcgccggc atcagtgacc ggctcccgcg
      961 accagggcgg tctcgagctc gctcgcgtcg agcaccgggc cgtcgacgca gaggcgcaca
     1021 tagccgtcgc gagtcgggac cacgcagccg aagcaggcgc cgaacccgca ggccatgccg
     1081 gactccatgg cgagctgggc gggaacgtcg cgctcggcgc agagggcgcg gaccgcctcg
     1141 agcatgggtg gcgggccgca ggcgaagacg gtgggcggcg cggagccggg cgcggcggcg
     1201 gcttcgtcca gggcctcgcg cagcagttcg gtcacgagtg cgtggcggcc ggccgagccg
     1261 tcgtcggtcg cgagggcggg ctccgcgtcg aacaggctcg ccgcctccgc gtagtccgag
     1321 ttgcggaagc cgagcatcac gggcgcggac gggccgagct cgtcgctgag ggcgaggatc
     1381 ggagcggcgc cgatgccgcc gccgacgaga agcgcgctgg gcgcagagac cggcgctgcg
     1441 aagccgctgg gcgcagagac cggcgctgcg aagccgctgc gcgcggcgga gcccgtcgct
     1501 gcggatcctc caggcgccgc ggagcccggc gctgcggagc cgctgagcgt cgcggagccg
     1561 ggcggggagc caccgggcgc cgcgaagccg atcccgagcg gcccgacgag ccggacctcc
     1621 tccccagccg ccgtccgcgc gagccgctcc gtgcccggtc ccacgtcctc gagcaggaag
     1681 tcgagccgca ggccggcgcc ggcctcggcg gagccgccgt ccgtctcgcc ggagccgccg
     1741 tccccctcgc gcacgcgcgc gtgcgagaag gctcgcggaa ggtacgggcg ctcgccgtcc
     1801 gagccccagc gctcggcggt ggagagcatg tagaactggc cggggtcggg ccgcgggccg
     1861 gcggcgtcga ggaccgtgag caggcggtag ccgccgagcg cgcgggactc gaccaccgcg
     1921 gcggagcggc gaccgaacgg ggcgagcgtg cgcgaagcgg agccgttggc agcgctcacg
     1981 tccgcgcctc gcgcggctcc ggcgcgccca ccgcgtgcag ctcctggagc gagcgcacct
     2041 cgggcgcctc gccgcgcccc gcacggatcg ctcgctgggc cgcgctcgcg ccggtcatcg
     2101 tcgtgatgca cgggatgcct cgcgagaccg ccgcgcggcg gatctcccag ccgtcggcgc
     2161 gggcgccagc gccggtgggc gtgttgatca cgaggtcgac gctgccggac tcgatccggt
     2221 cgaccacgtg cccaggcccg ccctcgttga ccttgttgat ccgctcgacc ggcacgccca
     2281 tccggcggat cgcctgcgcg gtgccgccgg tggcgaggat cttgaagccg aggtcgtgca
     2341 gcccggccgc gagctgcgtc gccgccggct tgtcgccgtc ggtgacgctg atgaagacgg
     2401 tccccgtcac gggcagcgac gcgccggccg cggcctgcgc cttgccgaac gccgccgggt
     2461 agtcgcgcgc cacgcccatc acctcgccgg tcgagcgcat ctccggcccg agcagcgagt
     2521 ccgcgcgcgc gaagcggttg aacggcagca ccgcctcctt cacggcgatg tgctcgcgcc
     2581 gcgaccggcg cgccggctcg agctcggcgt cgagatcggc gagcctctcc cccaggatca
     2641 gccgcgtcgc gatcttggcc agcggcacgc cgaccgcctt ggagacgaaa ggcaccgtcc
     2701 gcgacgcgcg cgggttggcc tcgatcacgt acagctcgtc gtcgccgtgc acggcgaact
     2761 ggatgttgat gagaccgacc acgccgagcc gcagcgcgat cttctcggtg gccgactcga
     2821 tccgcctgat catgtcctcg ccgagcgaca tggccggcag cacgcaggcc gagtccccgg
     2881 agtggacgcc ggcctcctcg acgtgctgca tgatgccgcc gatcgccatc gtctcgccgt
     2941 cgcagagtgc gtcgacgtcg atctcgatcg cgttctcgag gaagcggtcg aggtagatca
     3001 ccgcgtcggg gccggggccc gtccggcgca ggtagtcggc cagctcctcg ccggagtaac
     3061 agatctccat cgcgcggccg ccgagcacgt acgacgggcg cacgatcaac gggtagccga
     3121 tgtcgccggc tatcgcgagg gcggcctccg gcccctctgc cgcggcccac ggcggcccgc
     3181 gaagaccaag ctcgtcgagg agcgagatga accgcgtgcg ctcctcggcc aggtcgatcg
     3241 actcgaccgg cgtgcccagg atcggcaccc cggccgcctt caggccgtcc gcgagcttga
     3301 gcggggtctg gccgccgaac tggacgatca cgccctccgg ccgctcgacc tccacgatcc
     3361 cgagcacgtc ctcgagcgtg agcggctcga agtagagccg gtcgctcgtg tcgtagtcgg
     3421 tcgagaccgt ctccgggttg cagttgacca tcacggcgtc gcgcccggac gcgcgcacgg
     3481 tcatcgccgc gtgcacgcag cagtagtcga actcgatgcc ctggccgatc cggttggggc
     3541 cggcgccgag gatcatcacc gcgtcgcgct cgccgcggcg gatctcgtgg cgcgggccgt
     3601 cggcgccggg gcgctcccag cctgagtagt agtacggcgt ctcggcctcg aactcggccg
     3661 cgcaggtgtc gactgacttg aacgtgcgcg ccagccccgt ctccgggttc tcgccgcgtg
     3721 ccagggcgcc gatctcggcc aggaaccacg ggtcgatgtg cgtgcgcgcg tggagctcgt
     3781 ccgcgctcac gccgcggcgg aacgcctcgt cgagcagctc gtagcgatcg tgggccggcc
     3841 gctcgagccg cgtgagcagc tcgtcggtgt cggtgggcgc ggtcggcagc acgtcgagct
     3901 cgcgcgatcg cttggccttc gcgaacgcct gcttgaacgt gcgcccgatc gccatcgcct
     3961 cgccgaccga ctgcatgtgc gtaccgagca ggccgtccgc gccggggaac ttctcgaacg
     4021 cgaagcgcgg ccacttcacg accacgtagt cgatggtcgg ctcgaagctc gcgggcgtgc
     4081 gctgcgtgat gtcgttgtcg atctcctcga gcgcgtagcc caccgcgagc cgcgcggcga
     4141 tcttcgcgat cgggaacccg gtcgccttcg acgcgagcgc cgacgagcgc gacacccgcg
     4201 ggttcatctc gatgacgacg atctcctcgg tctccggatt gaccgcgaac tggatgttcg
     4261 agccgccggt ctcgacgccc accgcccgga tcacggtgat cgcctggtcg cgcagcgcct
     4321 ggtactgcac gtcgctgagc gtctgctgcg gcgccaccgt gaccgagtcg ccggtgtgga
     4381 cgcccatcgg gtcgatgttc tcgatcgagc agacgatcac cacgttgtcg ttgcggtcgc
     4441 gcatgagctc gagctcgtac tcgccccagc cgatcaccga ctcctcgacg agcacctgcc
     4501 cgatcggcga cgcggcgagc ccctccgcca cgcgctcgcg gtactcggac tcggtgaacg
     4561 ccgtgccgcc gccgtggccg cccatcgtga agccgggccg catgatcgcc ggcaggccga
     4621 ccttggccag cgcgcgctcg ccctcggcca tgctcgtgac caccgtcgag cgcggcaccc
     4681 gcaggcccgc cgcctccatc gtctggcgaa acagcagccg gtcctcggcg cgccggatcg
     4741 cctcgtagtt cgcgccgatc agctcgacgc ggtggcggcc gagcgtgccg tcctcccaca
     4801 gcgcccgcgc gaggttgagc gcggtgccgc cgccgagcgt ggggagcagc gcgtccggct
     4861 tctcgcgctc gatcaccgcc gccaccgact caggcagcag cggctcgatg taggtggccg
     4921 tggcgaactc cgggtcggtc atgatcgtgg ccgggttcga gttgacgagc acgacctcgt
     4981 agccctcctc gagcagcacc ttgcacgcct gcgcgcccga gtagtcgaac tcggccgcct
     5041 ggccgatcac gatcggcccg ctgccgagca cgaggatctt cttgatgtcg tcgcgcctag
     5101 gcattggcgg tggcgaggcc gtcgaggaag cggtcgaaga ggtacagcga gtcgtgcggg
     5161 ccgggtcccg cctcggggtg gtactggacc gtcgaggccg gcacgtcgcg gagcacgagc
     5221 ccctcgaccg tgcggtcgta gaggttgagc tgggccagct cggcggcgcc gaagtcggtc
     5281 tcccagcgga tcggctcgtc ggtgtcgatc gtgccctcgc cggcggggcc ctgcaccgcg
     5341 aagccgtggt tctgcgaggt gatgtcgatc cgcccggtgg cgaggtcctt gaccgggtgg
     5401 ttcgcgccgc ggtggccaaa cgggagcttg aaggtctcga ggccgacggc gcggcagagg
     5461 agctggtggc ccatgcagat cccccacacc ggcaccttgc cgaccagctc gcgcacgttt
     5521 gcgacgacgt ggtccatcgc ggcggggtcg cccgggccgt tggcgaggaa gaccgcgtcc
     5581 ggccggcgcg cgagcagctc ctccgcgctc gtgccgcacg ggtgcagctc gagccgcacg
     5641 ccacgctggc gcaggttgcg cacgatcgat cccttgatgc cggtgtcgat gccgaccacc
     5701 agcgggcccg ggttgtccgg gtcgagcacg atcggctcgc tcggcgtgac ctcgcaggcc
     5761 aggtcgcggc cgtccatcgg cggctcggcc atgacccgct cgcgcgcctg cgactcggcg
     5821 agcgacgccg ggaaaacgcc gccgcgcatc gcgcccttgt cgcggatgtg gcggacgagc
     5881 gcgcgcgtgt cgatgccgga gagcgccggg atcccgcagt cggcgagcca ggtgagccag
     5941 ccgagctcgg cgctcggcgc gtcctcgcgg tcgacgccct cgcgcatgac caccgcgcgc
     6001 gcgtggatcg cgtcggactc catggcggcc tgcgacacgc cgtagttgcc gatgtgcggg
     6061 taggtgaaga cgatgatctg gccgcggtag ctcgggtcgg tcaccgactc ctggtagccg
     6121 gacatcgcgg tgttgaagac gacctcgccg gtgctcgcct cgggcgctcc gaccatgtcg
     6181 ccgtcgaagc gcgtgccgtc ctcgagcagg atgtagccgc tcacgcgacc gccccgatcg
     6241 cgaacgagcg ctcgcggtag gcgacctggc cgccggccac cgtcatgcgc acgcggccgg
     6301 tgagccgctg ccccgcgaac gcgcagttgg ccgaccggct ctcgtagccg tgctcgccca
     6361 cctcccactc ggactcgagg tccacgaggc agacgttcgc gggcgagccg ggcgcgagcg
     6421 tgggagccgg gatgccgaac gggataccgc cggcggtcat gcgctcgacc aagagcgcca
     6481 ggtcgatgag gccggtgagc accagccgcg tgtgcagggc cgcgaacgcg gtctcgagtc
     6541 cggtcacgcc catcggcgcc acctcgaacg gctgctcctt ctcctcgcgc gcgtgcgggg
     6601 cgtggtcggt ggcgacgcag tcgagcgtgc cgcttcgcaa cgcggcgatc agcgcctggc
     6661 ggtccgcctc ggcgcgcagc ggcgggttca tcttgaagtt cgcgtcaagc gagcggaccg
     6721 cttcgtcggt caggcacagg tggtgcggcg tggcctcggc ggtgatctcc acgcccgccg
     6781 ccttcgccgc ctcgatggcg gccaccgatt cgcgcgcgga cacgtgctgg acctggatgc
     6841 ggccgccctc gtagcccgcg atcgccgcgt cacgcgcgat ggccgtcgac tcggagatcg
     6901 acgggatgcc gctcaggccg agcagcgcgg agaccgcgcc ctcgtgcatc gcaccgtcgc
     6961 ccgacagggt cgggtcctcc tcgtggaggg cgagcacgcg gccggcgagc cgctggtact
     7021 ggagcgcctg gcgcagcacg cccgcgctct ggaccgggcg gccgtcgtcg gtgaaggcga
     7081 acgcgccggc gtcggccagc tcggccatct cggtgagctg ggccccgtcc tggccgcgcg
     7141 tgatcgcggc ggtgaagccg gtcgggatgc gggcctcctc gcgcgcgcgc tcgcgcagcg
     7201 agctgaggac cggcgccgag tccaccaccg ggtcggtgtt gggcatcgcg aggatcgcgc
     7261 agtagccgcc cgcggcgccg gcgcggctgc cggtggcgat gtcctcctcg tcctcgcggc
     7321 cgggcgtgcg caggtgcacg tgcggatcga cgaacgcggg cagcgccacg agcccctcgc
     7381 cgtcgacgac ctcggcgccc tccggggcgt cgagcgcgcc cggtgcgccg agctcggcga
     7441 tcgcgccgtc gcggatcagc aggtcgccgg ggcggtcgag gtccgtgcgc gggtcgagca
     7501 gccggacctc gcgcacgagc acgtcgccgc cgggcaccgc tgcgaagtcg agctgggggg
     7561 cgctcatgcg ggctgcggct ccgtggtggc gccagggcgc gcgaagcgtg ccgcggagga
     7621 gcccccgccg ccgccggcga gcaactcgta gaggacggcc atccggacca cgaccccgga
     7681 ctcgacctgc tggacgatga gcgcctgcgg cgagtcgacc acctcgcccg ccagctccac
     7741 gccgcggttg accggccccg ggtgcatcag gagctggctc ggcgccagcc ggcgcccgtc
     7801 gatctggtag cgcgcggcgt actcgcgcag cgaggggacg aacgagccct tcatgcgctc
     7861 gttctgcatc cgcagcgcgt agatcacgtc ggcctcgccg atcccgtcga gcgaccagcg
     7921 cacctcgcag ccgagcgact ccacctcgcg cgggatcaac gtgggcggcc cgcagaccgt
     7981 cacctcgcag cccatccgcc cgaacgcgag gatgtccgag cgcgcgacgc ggctgtgcag
     8041 cacgtcgccg acgatccaga tgcgcagccc ctcgagcccg ccgagccggc ggcgcagggt
     8101 gtagacgtcg agcagcgcct gcgtggggtg ctcgtgcttg ccgtcgcccg cgttgaccac
     8161 cgacgccgcg ctccagcgtg cgacgagctg cgcggccccg gcgtgcggcg cgcggatcac
     8221 gatcgcggcg gggtcgtacg cgctcagcgt ctggacggtg tccttgagcg actcgccctt
     8281 gtccaccgac gacccgaccg acttgatgtt cacgaggtcg gcggagaggc gcttggccgc
     8341 cagctcgaag ctcgagctgg tgcgcgtgga cgactcgtag aagaggttga cgacggtgcg
     8401 cccgcggagc gtgggcacct tcttgatctc gcggccggag acctcggcga agctctccgc
     8461 ccggtcgagg atgcgctcga tgtcttcacg cgttagcgcg tcgatcgaga tcaggtcctt
     8521 cctcatgcca gcacctccgc gccctgcgtt gcgatcgtga cctcgtcgac cccgtcgagc
     8581 tcctcgagcc gcacgttcac gcgctcgttg cgggccgtcg ggaggttctt gcccacgtag
     8641 tccggccgga tcggcagctc gcggtggccg cggtcggtca ggacggcgag ctggacccgc
     8701 tcgggccgcc cgtagtcgaa gaccgcgtcg atcgcggcgc gcacggtgcg gccggtgtag
     8761 agcacgtcgt cgacgaggac gatcgtgctg ccgtccaccg ggaagtcgat gtgcgaggcg
     8821 tggaccaccg gctggcggtc gggcgcgcgg gtggcgaggt cgtcgcggta gaacgagatg
     8881 tcgacgtcgc cgagcggcac gtcgtcaccg gtcagctcgc agagcagctc gtgcacgcgg
     8941 ccggcgagga tcgccccgcg cctgtggatg ccgacgaccg ccacgggctc gccggagttc
     9001 ttctcgacga cctcgtggga tatgcgcacc agcgtgcgcc ggatgtcctc ggcgtcgagg
     9061 acgaccttct cttcgttcac gtcttccttc ctggcctcac gggaccgggt taaaggtgcg
     9121 acgcggggaa ccgtatcagc ggctccggcg gctcgatcgg gaacccgaag tcagaaacgt
     9181 ccctattagg gacgcaatcg acttcacccc tgagaccctc cctccctcac tgctgtcgtt
     9241 ggccggaatc ctcgcggcgg acgtgcttcg gctccccgcg ctcggggaac gggacctcga
     9301 cgcggtcgaa gtcgcgcggg aggatcgtgc gctcgaagac agcggtggcc tcctcgagca
     9361 gcacccccgg cgggtggcgc ggcgatacgt ggttgagcgc gagcagcttc acctcggcct
     9421 cacgcgcgag ctcggccgcc tggcgggcgg tcgagtgggc ggtctcggcc gcgcgctcga
     9481 cctcctcgtc gctgaacgtg gcctcgtgca cgagcaggtc ggcgccgtgg gagaccgcca
     9541 cagtgagcgg cgagggggcg ccgtcgccgg cgagcacgac cttgcgcccc ggccgcgact
     9601 cgcccaccac ctgctcgggg ccgacctcgc cgtcgcttcc cgggacggcc tcgccgcggt
     9661 gcaggcggcc gaagtccggg ccgggcttga cgccgagctc gagcgcgcgc cgctcgtcga
     9721 agcggccgag gcgctcgtcc tcgaccagcg cgaacccgta ggcgcgcacg cgatgggtca
     9781 tgtcgaaggc ggcgatccgg tagccgtcgc gcacgagctc ctcgttcggc tcgagctcga
     9841 cgaggcgcag ctcgaacgcg gcgcgcccga tcagcggcgc gagcgcgcgg aagagcgcgc
     9901 gcaggccggt cggtccgtag acggtgagcg ggcgctcgcg gccgcgcagg ccgaacgtct
     9961 tgatgatccc cggcagcccg aggatgtggt cggcgtggaa gtgcgtgatg aagatctcct
    10021 cgagctcgat cagcccggtc gagcgcagga gctggcgctg ggtcccctcg ccgcagtcga
    10081 acagcagccg ctcgccgccg cgccggatga gcgtcgaggt caggccgcgc ccggccgtcg
    10141 gcgcggaacc cgcggttccg acgaagagga cgtcgaggtc catggcgcct atctaatcga
    10201 gccggacgtg ctcgaacgcg acacgcccgc gcgcgacgcg cgcgatgccc atcgtgtggc
    10261 gcggctggcg gcgccggtcg gtggggctgc ccgggttgaa gatctggaag ccgtcgcgct
    10321 cctcgtgcag cgggatgtgg gagtggccga agacgaccgc gtccgcctcg ggaaagcgca
    10381 gacgcatgcg ctcgagccgc ctcttcgacg ggccggcgtc gtgcagcacg gcgatgcgcg
    10441 ccggtcccgc gtcgacgacg agcgactccg gcagccggcg gcgcagctcc tcgctgtcca
    10501 cgttgccgtg caccgcgcgc accggcgggc cgagtgcctc gatctcagcc agcgcctccg
    10561 gcgtgctgat gtcacccgcg tggatcacga ggtcggcggc gcgcatccgc tcgacgcagt
    10621 cgtcggggat gcggcgcgtc ccgcgcggga gatgggtgtc ggagacgatc gcgacgagca
    10681 ccggcgcgag actagactcg cggggctcgc gcccgtagct cagtggatag agcgctgccc
    10741 tccggaggca gaagtcgctg gttcgaatcc agccgggcgc gcctctcccc caaagcggtg
    10801 attgacagcc cgccgcgcgc ataagagggt cgtgaggagt gcgggccgac ggagcggctc
    10861 gccggactcg ccacggccgg caggggacta ctcccagggg gaccgtggac ttgccgaccc
    10921 gccgatgcat cctgcgatcg atcgcggtcg ccgctgacct gatgctcctg ctggcgccgc
    10981 ccgcgcacgc actcgacggc ccgcacaact tccgcaacgc caacatcgcg gaccgcgccg
    11041 aacagcgcgc caacggaacc tgggccggtc agtgcctcgt gttcgcgtcg gacatgatcg
    11101 tcgaggccgg cgggccgcgc ttctggttcg gcgacaacac gagcacctac caggcgcagt
    11161 gggcacagcg cgcggcgagc gtcggctctc tcgccgaggc tcggcgcggc gacgtcatcc
    11221 agtggggcgg cggcgccggt gggccgggcc tgcacacggc gatcgtcacc gcgcccgggt
    11281 cgaacccgag cgtgatcgac agcaacgtcg gatgggacga gcgcgtccac cgcggcagct
    11341 tcagctcgcg caacggctcc gccacgccgt accggatctg gcgggtcggg cgcgacgact
    11401 cccccccgcc tccgcctccg acagacgccg gcaagccatt tggcaacgtc gacgtgctca
    11461 ccggcgcgtc cggcgggcac gtacaggtcc agggctggac gatcgacccg gacgagagga
    11521 cggcctcgac cgacgtgcac gtctacatcg acggcccagc cgggtcgccc ggcgtgcgcg
    11581 gagtcaacct cggcgccgcc gacatctcgc ggcccgacgt ggcccaggtc cacggcgggt
    11641 ctggcgagcg ccacgggttc ggcgcggcca tccccggcgt ggggccgggc aaccacagcg
    11701 tctacgtcta cgcaatcaac cgcgctggtg gcggcggcaa cccgctcatc gggagcacga
    11761 ccgtcaacgt cccagccgac gccgccggaa gcccgttcgg cgcctacgac gagctgctcg
    11821 agcgcgccgg ctacaaggcg gacgtgcacg gctggaccat cgatccggac gcgcccggcg
    11881 cggccactga ggtgcacgcc tacgtggatg gcccggccgg cgtcggacga gggatcaacc
    11941 tcggtgacgc cgacgtgtcc cggccggatg tggacgccgt gcaccacgcg ggcgccgcgc
    12001 acgggctcga cgagcggctg tcggacctcg cggccggtgc gcacacggtg tggctctacg
    12061 cgatcaaccg cgcgggcagc ggcgacaaca tcctcctcgg cgccaagcgc gtctggatct
    12121 cggggccgag cccgatcggc cacctggacg tactcgcggg agcgctcccg gggtcgatcg
    12181 cgctgaatgg gtggacgatg gacccggatg caccgacgtc cccgaccgag gtgcacgtgt
    12241 acatcgacgg tcccgccggc tcgtccggcg cacgcggcgt gaacctcggg gcggcggacg
    12301 tgctccgccc ggacgttgcc gacgtgtacg cgggcggtgg cccggaccac ggcttcagca
    12361 ccgcgatcca gggcgtgccc gcgggaccac acgccgtcta cgtgtacgcg atcaaccgag
    12421 ccggaggcgg cgacaatccg atgctcggct cgacgacggt gaacatcccc gagaagcccg
    12481 ccgggacccc gttcggctcg ttcgacaccg ccagcggcgg tgccgggctg gcgagcatca
    12541 ccggctggac ggtcgatccc gacgcgccca ccctgccgac cgacatgcac acctacgtgg
    12601 acggcccggc cggctccgga gctcgtggca tcgacttcgg cgcggcggat cgcgcgcggc
    12661 ccgatgtcgg ggcggccaac cgaggcgtgg gcgattccca tgggttcgag aagaccgtcg
    12721 gcgacctcgc tccggggcgg catgttctgt gggtctacgc gatcaacagg gcgggggatg
    12781 gcggcaaccc cctcctgcgg acgctcccgg tcacggtggg tcagccggcg gccgccgctc
    12841 cgcaaaccgg cgaggggacg gtcatcccgc cgagcggcca acccagcctc gacacgtccg
    12901 cgtgcgcaac ggccgagcgc gcgaaggcga aggcgtggaa gaagatgtcc tccgcgaagc
    12961 gggcgtaccg caagcagcgc accgccaagc ggcggcgcca gtacgagaag acgcggcgcg
    13021 cctacagcac cgcgaagaag cgcgcgacca agctctgctg accgccgccg cgccttcgcg
    13081 gcgaaccggc ggacgctcgg gtggcgctga tctaggctcc ccgcgatgag gcattggcga
    13141 gcggtacccg gggcagtggt gatcgcgttc ctgctcgccg ccgcgcccgc cagcgccgcg
    13201 aagcagtgct ccgagccgga gcgcgactgg cagcgcgcga cacccgccga ggcggggctc
    13261 gacgcggcca agctccagga cgcgatcgac tacgccacca cgcagtcctc ctacgcggtg
    13321 cgcgtgtacc gccacggctg cctggtcggc gaggatcgca ccgcggccgc cagcgacaac
    13381 ctcaccttcg agagctggtc gatgggcaag tccgtcacct ccctgatctt cggtcgcgcg
    13441 atgtcgctcg gctacatctc gccggacgac ccggtcggct cgctcgtgcc cgaggccgac
    13501 gcggcgcacg gctccatcac gatgcggaac ctgctgacga tgacgtcggg cctgcactgg
    13561 aacgggttcc gcgactacaa cctcttcacg atgcccgacc gggtgcacga cgcgctcacg
    13621 ctcccgatcg accatcccgc cggcacctac ttcgagtacg cgcagagccc ggtcgcgctg
    13681 atcgcggagg cgatcggccg cgcggtcgac gaggacgtgc agagcttcgc ccagcgcgag
    13741 ctgatggacc cgctcggcat cgcggcctcg gcgtggtact gggagcgcga cccgtccggt
    13801 cacgtgcagg ggttcacggg cgtgaacatg cgccccggcg acttcgcgcg gctcggcgag
    13861 ctgatgcgcc gcaagggcat gtggcgcggc cggcggctgc tcgcgaagcg ctacgtgcag
    13921 gagtccgtcg ccccgaccaa gacgaacggc tgctacggct tcctgcactg ggtcaacgcc
    13981 ggggccccgt gcgtcggccc gacgatcacg agccgtcccg tcgaggaggg gcgcgacttc
    14041 cccgacctcc ccgccgacat gttccagttc tccggactgt tcgggcagcg ggtgacgaac
    14101 ttcccgtcgc tcgggatcgt gttcgtgcgc accggacagg atccgagcct ccttccggcg
    14161 ggcaacgccg agtgggagca cgacacctac gtgcgcctgc tcgctgccgt gaccgaccag
    14221 aaggtcgagc agccgggcgc tccgtcgcag ggcccgcctc agcaggaggc ggactacggc
    14281 ttccagaatg ccatccagca cccggaggac tacggcaagg gcaccgtgct gttccaggac
    14341 ccgctgcccc cggccggccc cgagcgcgcc cgctctccca tcccgctcca ggacgagact
    14401 cgcgcgagcc gtaccggcgc cacgacgatc ctgatgctgt gccccggcaa ctggcccggc
    14461 cgcccggccc cgcgctgcac gggcaccgcg aagctcgccg gcacgcagag gtcgctcgcg
    14521 tacgacacgc gcgcgggcac caacctgaag ctccgcttcg tgctgaagga caagacgctg
    14581 cgttcgctgc ggcgcacgcg gcgcatgagc gcgcgcgtga cgaccgcgac cgcggcgccc
    14641 ggcggggcca ccccgggcgc ggtcacggtc gtggtccgcg ccccgcactg acccgcgcgg
    14701 cgcctgtgtg gcacccggcg gagcgggtac gcaccctctg agggcaatcg accagaggag
    14761 agccacatgg gcaccgacga gaccggccag gtaaccggca ccaaggacaa ggactacaac
    14821 gtcatctggt tcacggagca gtgcctgagc aacgtgctgc ggctcgagca gtacgtccag
    14881 gacgccgagc gcgacgggga cagcgagctc gccgagttct tccggcgcgc gcaggagacg
    14941 agccgcaagg gcgccgagca gggcaaggag cagctcaaga agcgcctcgc gggctgacct
    15001 agccgcgcgg gccgcgcggg tggccctaga tttcatccgg tgagcgcgct cgccgagcac
    15061 agctacacga cccgcggcgg gatcgcggtc cgccgccgga ccgagcccgt cgggatcgag
    15121 gccgcggtcg agtcgatcat cgaccggctc gactcgcggc tcggcgtgct gctcgcctcg
    15181 cgctacgagt accccggccg ctacacgcgc tgggacatcg ggttcgccga tccaccgctg
    15241 atggtcacga gccgcgggcg cagcgtgcgc gtcgaggcgc tcaacgagcg cggtcgcgtg
    15301 ctgctggcgc cggtgcgcgg cgcgatcgac gcgctcgagg ccgtagagga cctggtggac
    15361 gacgactcgg gcttcaccgc gcgcatcgct acgcccaccc gccgcttcgc cgaggaggag
    15421 cgcagccgcc agccctcggt gttctccgcg ctgcgcgcga tcatcgagct gttcgcatcg
    15481 cccgaggatc cccaccacgg cctctacggc gccttcggct acgacctcgc cttccagttc
    15541 gagccggtcg agcagcacct cccccgcacc gccgatcagc gcgacctcgt gctgttcgtg
    15601 ccggacgagc tcgtggtcgt ggaccacaac cgccacctcg ccgagcggtt cagctacgac
    15661 ttcgaggccg gcggcgcaag cacggagggc ctgccgcgcg agggtcgcaa cgagccctac
    15721 cgccccacca ccgagccgcc gcccgtgcgc tgcgaccacg agcccgggca gttcgccgat
    15781 ctcgtccgca aggcgcagga ggcgttccgc cgcggcgacc tgttcgaggt cgtgcccggc
    15841 cagacgttct acgagccgtg ccccgacccg ccgtcggaga tcttccgccg cctgcgcgag
    15901 cgcaaccccg ccccgttcgg cgcgctgatg aacctcggcg agcaggagta cctgatcggc
    15961 gcctccccgg agatgtacgt gcgcgtggag ggtcgccgcg tggagacctg cccgatctcc
    16021 ggcacgatcg cgcgcggcga cgacgtgctg ggcgacgccg accagatcct cgagctgctg
    16081 aacagcgcga aggacaccgc cgagctgacc atgtgcaccg acgtcgaccg caacgacaag
    16141 tcgcggatct gcgagccggg cagcgtgcgc atcctcgggc gccggcagat cgaggtctac
    16201 tcgaaggtga tccacacggt ggaccacgtc gagggctacc tgcgcgacgg gttcgacgcg
    16261 ctcgacgcgt tcctcaccca cgcgtgggcc gtgaccgtga ccggcgcgcc caaggtgtgg
    16321 gcgatgcggt tcatcgagga gaacgagcgc tcgacgcgcc gcttctacgg cggcgctatc
    16381 ggtgtggtcg gcttcgccgg cgggatgaac acgggcctta cgctgcggac cgcgcgggtc
    16441 aaggacggcg tggcccgggt gcgcgccggg gcgacgctgc tgatcgactc cgaccccgac
    16501 gaggaggagc gcgagaccga gctcaaggcc gcggcgttca tcgacgcgct gcggcgcccg
    16561 cgcggcgtcg acgagacccc cgtccgcagc gccgacctcg acccgcaggc cggcgccggg
    16621 cgccgcatcc tgctggtgga ctacgaggac tcgttcgtcc acacgctcgc gaactacctg
    16681 cgccagaccg gcgccgaggt gaccaccgtc cgcggcgccg acgacgacca gctacgcgat
    16741 gccctcgacg cggtcgggcc cgacctcgtg ttcctctccc ccgggccggg cgcgcccggg
    16801 gacttcgacg tgagccgcgc gatcgacctc gcgctcgacc gcgagctgcc ggtcttcgga
    16861 gtctgcctcg gcctccaggc gctggtcgag cacttcggcg gcagcctcgg gctgctcgac
    16921 taccccatgc acggcaagtc gtcggtgatc gacgtgcgcg gcggctggac gttcgagggc
    16981 ctgcccaagc gcttcgaggc agggcgctac cactcgatct acgccgcgcg cgacgcgctg
    17041 ccggcggagc tgaccatcac ggccgagacc gacgacgggg tgatcatggg cgtcgagcac
    17101 accgagctgc cggtggccgc ggttcagttc caccccgagt cattgatgac gacccgtcgc
    17161 gacgtcggcc tgcggctgat cgagaacgtg gtgcgccggc tggcggctag gcgcccggcg
    17221 cctgcggcgg cagctgcgcc tccgcaggct gctgcgccgc gtcctgcccg agcggagtag
    17281 aggccccggg cccggcggcg agcgagcctc cgccgcccgc gccgccctcg gtctcacgct
    17341 cccaaacctt gttcagctcg gtctgcgcgt agaagaggcc gaccgggaag aagatgacca
    17401 tcgcgacgca ggccatgacc gcgttgatgc tctcgcctat gccggagatc cgctgagcgg
    17461 cgccgatccg cttgcccgtg ttcagcgacg agatgacgaa ggggatcacg acgaacgcgc
    17521 ccggcagcag cgcgagcagc gagttcgtcg ggttgtcgcc cagatccttg gtgccgcggg
    17581 cgcggccgag ggcggccagg tcgcggttgg tcacgaagta ccagtagatc ccgtagatcc
    17641 cgaacgtgac caggtcgagc agcgcgctcg tgacggggtt gcggaccttt acagtggcgt
    17701 ctccgccgcg aatttgaagc tcagggggtg ccatgagtga gccatgctcg catacagacc
    17761 ggatagaagt cggcgtgccg gacgaggtcg cgggctgtga ggagtgcctg aagaccggcg
    17821 accgctgggt ccacctgcgc gcgtgcctga cgtgcggcaa ggtcggctgc tgcgattcct
    17881 cgccgaatcg ccacgcgcga aagcacgcgc acgaggagga tcacccggtg atcgcctcgg
    17941 cgcagccggg cgagcactgg ggatggtgct tcgtggacga ggtgatgctg agcgtctgag
    18001 ggccgggccg cgacggcgcc cggtcaggac gccacgccgc acgcgcccgc cgggtcgccg
    18061 gacagctgcg cccgatacca ctcgtaggtg gccgcgagcc cgcggtcgag gtcccacgca
    18121 ggggcccagc cgagctccgt gcggatcgcg ctcgagtcga gcgcctggcg gtccgcgccc
    18181 gccgcggccg cgccccgcac gtcgggctcc aggtcccgcc ccgacgcgcg gacgaggcgc
    18241 cggacgacgt cgatgaccgg cgccacctcg ccgctccccg cgttccacgc ccggcccgcg
    18301 tgcgcgtgat cgtcgagcgc ggccgcggcg gccaggtacg cctccaccgc gtcctcgacg
    18361 tagagcagct cgcgctcggg ggtgccgtcc gagcgcagca cggggcgctc gccgcggacc
    18421 agcgcccggc aggcgtcggg aacgaggcgc gagtagctca ggtcgccgcc gccgtagacg
    18481 ttcgccatgc gcaggaccgc gaccggcagc ccgtgcgtgg ccgcgtagga gcgcgcgatc
    18541 gagtcggcgg cggccttcga gacggcgtac gggtggacgg ggcgcagcgg gtcgtcctcg
    18601 cggaacgggc gcgcgccggc ggccgggccg taggcgtgga gcgacgaggc gacgacgatg
    18661 cgctcgaccc cctcccccgc cgcgcggcac gcctcgatca ccgtgtaggt gccgcgcacg
    18721 ttcgcctccc aggtcgggta gggcgagcgc gcggccacgt cggcgatcga ctgcgaggcg
    18781 aggtggaaga ccgcgcggac gcggtgctcg cgcagggcga gcaggaccga atcgtggtcg
    18841 ccgatgtcga gctccacgac ctcgcagcgc tcctcgatcc ccgctctgcg aaagcggctc
    18901 tcggcggccg ccgcgcgccg cggcaccacg acgcgcgcgc cctccgcgag cagtcgctcg
    18961 gcaagccacg agccgacgaa gccctgagcg cccgttacca gcaccgggac gccgtcccag
    19021 cggccgctca tgcgggcgtg gctccgggca tcgcggcaga gtagcggcgc acccgctgcg
    19081 ccaggagggc ggcccagagc gcccacaggg cgagcatcgg cacggtgtac acggcccaga
    19141 tcgcggcctc gctcgacacg tccaggtaca gaagcagcag cgcgggaccg gccagcgcct
    19201 ggatggcggc ggcggctcgc aactcgccgc gggcgagcag gaacggcagc ccccagatca
    19261 ggtactggat gaagaagttc acgccgatga gccagacagc gagccagacg atcgtggcgg
    19321 cgcccagcgg ctcgggccgc gtgcgggcga gcagcgcggc ggttgcgatc aggccggcgg
    19381 ccacgaccac gccgccgccg tcgatcagcg cgtcggtggc cgcgttcggg ctgtagtcgt
    19441 ggccggcctg ccagaacagg gcgaggtcgg gctggacgag caggctgagg ccgccgaggc
    19501 cggggaagcc gcggtaggtg aggtggtccc agacggcgcc cgggtcgtgc gcgaggaacg
    19561 gcgcgatgag cgcgagcggc accgccgccg cggcggcgag gagcgtggcg gcctcgcgcg
    19621 gcgagcgcac cgagggcagc agtgcgagca ccagcagcag cgggaccgtc ttcaccgacc
    19681 ccccgaggcc gagcagcagc ccggcgtgca cggcacggcg cgggcctccc cgctcccaga
    19741 cgacgaacgc ggccagcgcc gggaggatcg cgacggagtc gatctgcccc tggtagcccg
    19801 ccacccccac gaacaccggg ccgagcgcga cggaggcggc cgcggcgagg cggatgtgct
    19861 cgctcgctcc gcgcatcccc agcaggtgct ggacgagcag cgcgatgccg acgtcggtcg
    19921 cgatcggccc gagccgcagc aggtgcagga agtcgatgcc cgtggcggag gcgacccccg
    19981 cgaagcccgc gacggccggg aggtagccgg gcgggtacgg ccagcgcagg tcgggcacgg
    20041 cggagtagaa gtcgagcgca tcgctcttca gcgcgtcgcg gacgatggcg aacgaggcga
    20101 ggtcctcggt gttcgtgtcg gcgaggatcg cgacgagcag ccgggcgagc gtgcccgccg
    20161 cgatcgtcag ccacagcagg cggcggccac gtaggctcag cctcgtggcg ggagcgctca
    20221 cagcggcggc gggagcgccg ccggaaggtg cgggtcgagc gcgcgggcgc gggcccagta
    20281 gcggcttgcg gcggcctcgt cgccgcgtgc ccgggccagg cgggcgcgca ggtgccacac
    20341 ccgcaggttc ttcggctcga gctcggccgc gtcggccagc agctcctcag cgcgctccgg
    20401 ccggccgcgc tcgtagttcg tgcgcgccat cgccagctcc cgtgccgcgc cggggttcag
    20461 cggccgcgag tcgtcgaaca ggcgctcgac ctcctcgacc ggccgctgtt cgatcgcggc
    20521 ggagaacgcg tcctcgaccc tcttctcgct gcggaaggtg acgacccccg cggccacgcc
    20581 cagggccgac agcacggcca cgcctgctcg cacgatcgat cgggtcggca cgcgcgcatt
    20641 atgggtggcg ggcgccgcgc tgctcgcggg gccggtcctg ctggcgttcg acagcggcgg
    20701 cacgggggtc tcgacgcagg cggccggggc ggtgctcgcg ttcgggctcc tcgtcgtgct
    20761 ggccctcctc gcctcgtggc cgctggtgga gcgctcgtgg ccgctgtatg cccttggcgc
    20821 actggccgcg ctgtgcgcct ggacggcgct gtcggcgagc tggagcccgg cgctcggcga
    20881 cgcggtgaac gacgccgacc gggtcgggtt ctacgtggcc gcgttcgcgc ttgcggtggt
    20941 ggtcatgcgc gcgccggcgg tgcggcgggt gacgccggac gcgctgatgt ggggcgtgct
    21001 cgtggtcgcg ctctacgcgc tcgcgggccg gctgctgccg gacgtggtcg aggtcaagcg
    21061 ggccccggtc gcgggcgacc ggctcgacca accgctcacg tactggaacg ccatggggat
    21121 cctcatgggg ttcggcgtgc tgctggccac cgcggtcgcg gcggacgagc gccggccgct
    21181 ggcctaccgc gccgcggcgt gcgcggcggc cgtgccgtgc gcactggcgt gctacctcag
    21241 cttctcgcgc gcctcgtggg ccgcggtggt ggccggcgtg gcggtgctgc tgctggtgcg
    21301 cccgcgcctg gcgccggcgc tggccgcgtg gctgtggggt gcggcggtgg tggtgctcgc
    21361 gctggcgctg cgggcgttcc cggctgccct gcacctggat cgcggggcgg atgcgcaggc
    21421 ctccgacggg ctgccggtgg cgcttctgtg cgtggggctc gccgtggcgg tgggcgtggc
    21481 gttcgcgctg gtggtgcggc ggcgcgaggc ggcggtgagg tccccggccc ccgccacgag
    21541 cgggccgccg cccggcccca ccttcgcccg cccccacggc tggctcgccg ccgccgccgt
    21601 ggccgtggta ctcggcgcgg gcctcgcggt ctcgttcgcc accgagcgca cggaggacat
    21661 ctcgaagtcg gccgcgcgcg tgaccacgct caagacctac cgcggggagt actggcgcgt
    21721 ggcgctgcga tcgttcgccg atcacccggt cgggggcgtc gggacggccg gcttccaggt
    21781 cgagtggatc cgggagcgcg aggagcgcgt gttcgccttc gacgcgcact cgctctacat
    21841 cgagacgctc gccgagctcg gcatcgtggg gttcgcgctg ctcgccgcgt tcgccgcctg
    21901 cgtggctggg gggctgcggc ggcacttccg cgcggcgccc ggggacccgc tgctcgccgc
    21961 cggcgccgcg gtgctcggcg cgttcgccgt gcatgcgggg atggactggg actgggagat
    22021 gcctgcggtc acgctgccgg cgctcttgct cgcggccgcg tgcgtgcagc ggccggcgcc
    22081 gtccggatga ggatcctcgc gatcggcagc cagtacccgc cgcaccacct cggcggctac
    22141 gagctggtct gggagtcggg gatggagctg ctgcgcgacg cggggcacga gccgctcgtg
    22201 ctcaccaccg acttcgcggt ggccgacccg gagccgcgtg acgaccctgg cttcccggtg
    22261 cggcgcgacc tgcgctggta ctggagcgac cacggcttcc cgcgcctcgg cgcacgcgag
    22321 cggctctccc tcgagcgcca caaccggcgc gtcctcgcac gcgcgctcga ggagcaccgg
    22381 cccgacgcgg tgagctggtg ggccatgggc gggatgtcgc tgtccctggt cgagcaggtg
    22441 cgccgcgcgg gcatcccctc ggtggccgcg ctgtgcgacg actggtggtc ctacgcgccc
    22501 gcggtcgacg cgtggacgcg agcgttcgcg acccgcccgc ggctagggcg cctggcgcag
    22561 gggctcacgg ggattccgac ccggcccgac ccggccgccg cgatcgggac ctgggtgtac
    22621 ctcagcgagg agctacgcac ccgcgccgcg gcggcggggg tcgagccgct ccactcgcgg
    22681 gtggccacgc gcgggccgga cccgatcttc gagcgtcccg ccgaggaccg gccctggcgc
    22741 tggcagctga tctacgtggg ccggatcgac gagcgcaagg gcgtggacct cgcggtcgag
    22801 gcgatggcgc tgctgcccga ggaggcgaga ctcgaggtga tcggcgacgg cgacgcggcc
    22861 gagctggcgc ggctgcgtgc ggccgcaccc gcggggcgcg tgcggttcga acgggtgccg
    22921 cgcggggagc tgccggaccg cgtggcggcg gcggatgcgc tgctgttccc ggtccgctgg
    22981 cgcgagccgt gggggctcgt gccgctcgag gcgatggcgg cgggaacgcc tgtgatcgcc
    23041 acgggccgcg gcggctccgg cgagtacctg cgccacgagg agaactgcct gatcttcgat
    23101 ccggacgggg gcccgccggc gctggccgcg cagatcgagc gcctcgcgtc ggaccccggc
    23161 ctgcgcgcac gcctgcgggc gggtggtgcg gcgacggccg ccgcccacga cccgcgcggg
    23221 ttcgagaggg cggtcgcgga ggagctggag cgagcggcag cgaagagatc cccgtgagcg
    23281 acgagcgccc cgcctacgtc tacctgctgc gctgccgcga cggctcgctc tactgcggct
    23341 ggacctacga cgtggagaag cgtctggcga agcaccaggc cgggaccgcc tcccgctaca
    23401 cgaagacacg ccgcccgctg gacctcgcgg cggcgttcga gctgccggac gcgacggcgg
    23461 cgcggcgcga ggaggcacgg atcaaacgcc tgccgcggcg cgagaagctc gcgctcgtgc
    23521 ccgcatcgtc ctgacgcgcc agcgctcgtc gcgcgagaat gccgggatgc caacccggcc
    23581 gcgcctcgcc cgctggatcc cgatcctgat cggcacggct ctcgcgctgg cgcttgtcgg
    23641 ctgggccgtc ttcgagctga tcccggaccg cttcgcgtcc accgaggggc tcactccgga
    23701 cggaagggag cgggcgcgca acgacgtccg gacgagcgcc ctgcagctcc tcgccggcat
    23761 ggtggtcgtc gccggtgcgg tgctctcggc cctcacgctg cgcaccaacc gcgagggcca
    23821 gatcacagag cgcttcacgc gggcgatcga ccagctcggc agcggcaacc tgccggtgcg
    23881 gctcggtggc atctacgcgc tcgagcggat cgcccgcgac tcgcagcgcg atcacgggcc
    23941 gataatggag gtgctcacgg ccttcgttcg cgagaacgcg cccgccgatc tagagcaggc
    24001 ggccgacgac gcccgcatcc gccccccggc cgacgtcgcg gccatcctca ccgtgatcgg
    24061 gcggcgcatc cgcgcgcacg agaccggggg tgcgccgctg aacctggctc gaaccgatct
    24121 gcgcggggcg cgcctcgacg gcgcggacct gcgtgacgtc atcctgagct acgccgacct
    24181 caggcacgcg gatctcgtgc gcgcacgcct cgagggggcg atgctggtga gggcacgact
    24241 cgaacgcgcg tctttcggcg tcgatcggac catgccggag tacggagcgc ggctggacga
    24301 ggctttcctg atgcgcgcgc acctcgccgg cgcgcacctc gagggcgccc acctccgcag
    24361 ggcggagctc agcaacgcgg gcctcatggg cgccaacctc gccggcgcgg acctcgacgg
    24421 cgcgtccctg ggtggagccg atctctccgg agcgaccgtc gggggcgcga gcttccgcgg
    24481 cgcgcacttc gcgagtacca cggtctggcc cgaggggttc gacccggagg ccgccggagc
    24541 gctctccctc gaccttccgg acgcctaggc ggcacgagta cgctccggcc gtgctcgcgg
    24601 cgagtcacga gcggctgctg gcgcggctta ccgacggcga tctcgtcctc gacgtcggcg
    24661 gtgggttcag cccgttcgca cgagccgact gtgtgctcga cctgctcccg tacgaccgtc
    24721 gcagggcggt gcccggcgag cgcttcacgg acgagacctg ggtcgagcgc gacgcgtgcg
    24781 accacgagtc ctggccgttc cccgacgcgc aattcgactt cgccgtctgc tcgcacacgc
    24841 tcgaggacgt gcgcgacccg gtcttcgtct gctcggagct ccagcgcgtg gcgcgagcgg
    24901 gctacatcga ggtgccgtcg cggctcgagg agcagagctg gggcgtggtc gggccctggg
    24961 tcgggtggag ccaccaccgc tggctcgtcg acgctggcgc ggattggatc cggttcgtcg
    25021 ccaagccgca cgccatccac ggccaggagg ccgctcactt cccgcccggc ttccacgcga
    25081 cgctgacgcc ggaggagcgc gtccagcagc tctggtggga gggcgccttc gacttctccg
    25141 agcgcgtctt caccgaggcg gccgagctcg acgcctacct gcgcgagccg gtcgacgccc
    25201 gcggcggccc ctcgcgcggg tggcgggccc gccgccggct cgcgcgccgc gtcagcggcg
    25261 gctgacgcag gcggacgact cgcccttcgc gcagccctcg agccggcgcg cgctcttgcg
    25321 gtaggccggc agcgcttcgc gcgccgcgaa ctcgttcgac ccctccgcgt accggacgag
    25381 ccccgagaag tcgaagatgt cgccgtccga ccggtacgac gacgcccacg tgtaccagta
    25441 cgccgcctcg atccgcagct tcctgcggtt cgccgcgagc gcgtcgtagg cgttcagcac
    25501 gagcttcgcg agcttgcggt cggtggtctg gaggtggtcg cggtcgcggc ccaccttgat
    25561 ccggccctcg gaggcggagg cgccgaactc ggtcacccgg atcggcttct tcccgtcacc
    25621 gcgcttctgc atctccttgc gcccgcgccg cacgatctcg acgaggttct tcggctcgcg
    25681 ggtgtacgcg ttgagcgcgg ccacgtcgaa ggccccgcgg atcccgccga gcttgtagag
    25741 cgaccgcagc gagatccagg acgagttggt cagccccgcg agcactacgc gcgcgcccgg
    25801 gtccgccgcg cggatcgcgt cgcgcgcgtc gcgcaggagc ttcccgtacc tggcctgcca
    25861 gtcgtcgcga ggcacccact ggtactccat gtcgggctcg ttccagatct gccaggtccg
    25921 cagtggccgg cgcggcaact ccgggtgctc ggcccagaat gtgcccgcct cgccgtagcg
    25981 gccgatcagc gtggtcaggt acgccgtgta ctgcttggga tcggcgggcg cggagtgctt
    26041 ctcgccggag acgatgcgcg cccagcgcgg ggtgtagatc accaccggaa ggaggtcgat
    26101 gccgcgcaac gccgcgcgcg cgaccagcgg gtcggtctcg tggaagctca ccgcgccgcc
    26161 ggcggtcggc tgcgcctcgc tccagtcgaa cagcacccgc gcggactcca ccccgttggc
    26221 cgccatcgcc cgccacatct cgtcctgcac gcccgccggg ccccacgagg cctcgcggtc
    26281 gtagtcgaca ccctggaagc cgaacggcgc ccggcgctca gcccccgcgg cgggcacggc
    26341 cgcgacgagg cacgcgacca gcgcgacgag gcagaggcgg agaggggacg ccaccacccc
    26401 gatcctaggg acgaggccgg ggtccgcgac gcggttcggc tactcgtgtc cgatccggta
    26461 cagcgtgagc cggtcgctgc gcgccaggac cgcgaagccc tcctcgcgcg cccagcgatc
    26521 gtcgtcggag tactggccgg gcaccgggca ctcctcgcgc gcgtagccac cgcgccccac
    26581 gagcaggagg tcgtagtgac cgcggcggat cgcggccgcc cagcggtcgc gccgcgagta
    26641 ctcgcgcaac tgctcgtcca cggtcggacc caggtagtcg acctcgttgc gcagccgctc
    26701 gccgaacgcc ggccagaccg gcgcgcgccc ctccacgctc cacacgccgg cgagcgccac
    26761 ccgctgcccg tccggcgcgt ggcgggcgat ccacgcgatc gcggggtcac cgtcctcgta
    26821 gcgaccgtcg ttgaactcgc gctggcgggc gttcccgacc gcgatcagca ccgccgcgaa
    26881 cgcgacggcc gccgccgccc gcgccacgcg ccgcgatcgg cgcccccagc cctcggtcgc
    26941 ctccgccacc ggccaccaga cgtagagggc gacgccgagt aggacggcgg gcacggcggc
    27001 caccacgccc aggccgacgt cgaacgagcg gtcgatcccc tgcacgaccg cggcgagggc
    27061 gagcagctcg agcgggaccc gcaggcgccc ggcccgcccc agcgccacgg ccgccagcgg
    27121 cgccgccacc agaagcgcgg ggaccaggta gcgcgcgttc gcgcccacga gcagcggctc
    27181 gccctccggg ccgaacgcgc tgtagggggt gacggcgtag ccgatcgcga gcaggaccac
    27241 gcacgccagc cccgcgacga gcgcgccgcg ctggcgcacc gcgaccacca ccgccccgac
    27301 cagcccgagc ccgatgaccg ctcccggccc cgcgaaggtc tcgcggtagg ccgggacgat
    27361 gtacttcgac cagacgtccc agtcgccgag gtagccggcg atggtgaagc ccgcgcactc
    27421 gcggatgaca tcgcgcgggg catcgaagat cgtgaccccg agcgcgctca gctccagcgg
    27481 gaacacgggg ttgccggaca ggacgaggtt gcgcacgagc cagaagccgc cggcgagcgc
    27541 cacgatcgcg gccaccggga cggcgtcgcg cagcggccgc ggccgcgcgg cgacgagcca
    27601 gacgccgagg accgccgcca cggcccacag cgcgtaccac ttcgtcccga acgcgaggcc
    27661 gagcgacagc gcggccagca gcagctccga ccttggtgca ccgcgcgcgt ggcgcagcag
    27721 gaagaggatc cccacgccca tcgtcgcgaa catggcggag tcggtcatcg cctcgccgtt
    27781 ggcggcgagg cccacgacgg gaatggtcgc gagcagcgag ccggcgagca tcgacgcggg
    27841 gcgcggcgcg cccagctcca ccgcgatcgc gtagacggcc aggcccgcca gcgcgatgta
    27901 gatcgggttc acgatcccga cgagccagtc gttgcgccac ggctgcacga cggccaggaa
    27961 cgtcaggtcg cccgtctgcg ggtagttgcc gttggcgagc agcgggttga actggtccac
    28021 gcgccagacc gtcccgctgc ggatccagcc ggcgacgttc ggcaggtgga acgtgagcga
    28081 gtcgatcccc tccggcggca gcacggtccg gttccacgcg ctcgccacga gccacagcgc
    28141 cagcgcgctg gccgccgcga agccgatcgc ccacgagacg gggctctcgc cgctgcgcgg
    28201 accgggctcg cggggatcct cgaaccattc gatcggggcc gcccgcaacc gcgtggcccc
    28261 gaccgcaagc gcgaccgcca ccgccgcggc gctccagcgc gagagcagcc cgaccaggcc
    28321 cggcagcagg tgcgacgccg acagcgccgc ggtggccagg accgccaccg ccagcacccg
    28381 cgtcacgccg tcgaggtgcg ccaggcgccg ccgctggagg accagcccgg cggcaagcgc
    28441 cgcccccgcc gtgacggcga ggaaggccag cccggctagg tagtccgcga acggcaccgg
    28501 cgcggcggct taccgccgcc tggagcgcta gccgccggcg acggccggca ggatggtgac
    28561 ctcgtcgccg tcctcgacgg gtgtctcgag cccgtcgagg aagcggatgt cctcaccggc
    28621 cacgtacacg ttcacgaagc ggcgcaggtc gccatcctcg gcgatccggt cgcgcagccc
    28681 gtcgtagcgg tcgtagaggc catcgaggac ctcccccacc gtgcgagcgt cgtcgacgct
    28741 ggcctcggcc tcgcccccgg tgacggcccg gagctgtgtc gggatcttga cggtgatgct
    28801 catcggccgc gcatcctacg caccggcgcc gaccggcgcc gcgaaggcct cctcgaacga
    28861 ccgcggcgtg ggctcgatct cgttgacctc gaacgtgccg cggaccgcgt cgagcgtctt
    28921 caggccctcg cccgtgatca ccagcacgac ccgctcgcca cgggcgatgt cgccacgctc
    28981 ggcgagcttg cgcagcaccg ccgtggtcac gccgccggcg gtctccgtga agatgccggt
    29041 ggtctcggcg agcagccgga tgcccgagcg gatctcgtcg tcggtgacgc tgtcgatccc
    29101 cccacccgag cgccgcgcca ggtcgagcgc gtagatcccg tcggccgggt tcccgatcgc
    29161 gagcgacttg gcgatcgtgt cgggggtgcg gaccgggcgg cagacgtcct tgcccgcctc
    29221 gtaggcctgc gcgaccgggg agcagccctc ggcctgggcg ccgttgaacg ccggcagctc
    29281 gccctcgacc agcccgacct cgatccactc ctcgaacccg cgcgcgatct tggtgaacag
    29341 cgatccggac gcgaccgggc agacgacgcg gctgggcagc tcccagccga gctgctcggc
    29401 gatctcgaac gcgagcgtct tcgagccctc ggagtagtac ggcctcatgt tcacgttcac
    29461 gaacgcccag tcgcggtcgc cggcgagctc ggtgcagagc cggttcacgt cgtcgtagga
    29521 accgcggacg gccacgaggt tcgtgccgta gacgccggtg gcgaggatct tctgctcctc
    29581 gaggtcggcc gggacgaaca cgtaggactc gagcccggcg gccgcggcgt gggccgcgac
    29641 ggagttcgcg aggttgccgg tggaggcgca ggccacgacc tcgtagccca gctcctgcgc
    29701 cttcgcgagc gcgaccgtga ccacgcggtc cttgaacgag tgggtggggt tggcggcgtc
    29761 gttcttgacc cagacctcgt cgaggccgag ccgctcggcg agccggtcgg ccttcacgag
    29821 cggtgtcgag ccggcaccga gcgcggactt cggcggccgc tcgaacggca ggaagtccgc
    29881 gtagcgccag atcgactgcg gtcccgcctg cacgcgccgc cgggtctcgg cggggtcgag
    29941 cgcgctcagg tcgtactcga cctcgagcgg accgaagcag cggtcgcaga cgaacagcgc
    30001 ctccagcggg taccgctgtt cacattcctt gcacttcagg gcctgggccg gcattcacag
    30061 cctcctagcg tctctgggtg tcaccgccgc tttcgctttg tgacggagcc tggacgctct
    30121 gccacatctc ccagaccgtt gccttggcgg cctgatggaa ttggcaccgt ttccgccgaa
    30181 gcggatggtt gccggggttt cgtagggcca tgtccctcca cccctctaga tgcgaacggc
    30241 tatgtcgaac ccgggaggat acagcctgtg gaggtcagtg tcatcgtccc ggcctggaat
    30301 gcggcgccaa ctctcgggcg cgcgctcgag gccctggccg cgcagcgatt cgacggatcg
    30361 tatgag
//
LOCUS       DASVPL010000126        29739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_289840, whole genome shotgun sequence.
ACCESSION   DASVPL010000126 DASVPL010000000
VERSION     DASVPL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 29739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29739
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289840"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..7990
                     /locus_tag="VF517_06480"
     CDS             107..7990
                     /locus_tag="VF517_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8052620.1"
                     /translation="MLLTDNALATSDAESPNLSQSALLGLLRSAHSENPGRFGVIDLD
                     GADASASALAGALAAEEPELALREGRALAPRLARASVEDDAAQAGAEAGGGAGAAGAG
                     PDPDGTVLITGGTGGLGALIAGHLVAEHGARRLLLTSRRGLEADGAKELVASLKELGC
                     EAEVAACDVADREQVEALLAAVPAEHPLTSIYHAAGTLDDGVIDSLDAERLQKVMAPK
                     VDGALNLHDLTEGAELSEFVLFSSVASTLGTPGQGNYAAANAFLDGLAHRRRSQGLAA
                     TSLAWGPWARAGGMLGSLNEVDLERWERAGISTLSDEQGLAMLDAARATAQPLLVPVA
                     LEGSALRAQAKAGTLPAIFSGLYRTAVRQSAEGGGSLATRLAASPESEWQAIVLEIVQ
                     THVAGVLGHSSGEAVDPQRAFKELGFDSLGAVELRNRLGQATGLKLPTTLIFDHASPA
                     AVAEFLRSKVGGAERAKKVTRGSRTRNDEPIAIVGMSARYPGGVRSPDDLWQLIVAGG
                     DAIGEFPEDRGWDLEKLIHPDPDHPGTSYTRHGGFVYDADRFDAEFFSISPREALALD
                     PQQRLVLEGAWEALENARIDPLSLKGSQTGVFTGVMYHDYGIAGMPPELEGFLGPGGA
                     GGGAILSGRLAYTLGLEGPAVSIDTACSSSLVALHVARQALEQGECDLALAGGVTVLS
                     TPIAFVQFSRQRALSPDGRCRAFGADADGVGWAEGAGLLVLERLSDARANGHEVLALV
                     RGSAVNQDGASNGLTAPNGPSQERVIRQALASAGLSPSDVDVVEAHGTGTSLGDPIEA
                     QALLATYGQKRSNGPLYLGSVKSNIGHTQAAAGVAGVIKMVEAMRHGLLPRSLYCEEP
                     SPHVDWSAGDIELLAWPVDWSPGERVRRAGVSSFGVSGTNAHVIVEEAPAPEPEPLVE
                     ANTHLPVLPIVVSARSEAALRGQAERVREWLYERVELEPIDVAFSLASHRAQLDRRAV
                     VLGSDRAELVAGLEALARDETDAAVVGQARTGKSVFVFPGQGSQWEGMALELLDLAPA
                     FAESLRACGEALAKYVDWSLEDVLRGAEGAPSLDRVDVVQPALFAVMVSLAALWRSYG
                     VEPSAVVGHSQGEIAAAHVAGGLSLDDAARVVCLRSQAVLDELAGKGGMGSVALSPED
                     AETRLQPYGERLSLAAVNGPASVVISGEMEALDELLAACEADGTWARKIPVDYPSHSK
                     AVERIEQRLAEELGPIEPKTGSVPFFSTVEAEVVDTATLDAGYWYRSLRNRVRFNDAV
                     ATLIEDGASAFLEMSPHPGLTVGVAAAADAAGAAERIAAVGSLRRGEGGLERFLASLA
                     EAHVHGVDVNWSQLFAGTGARVVDLPTYAFQRERYWLEAGTNLGDLAAAGLGSVDHPL
                     LSAMLASPGNRGASFTGRVSLAAQPWLADHAVFDRVLFPGTAFAELLLAAGAELGCPV
                     VEELTLEAPLLLAEGDEVALQVVVGEDDGRGRRQVEVYSKPPAHAEHEDDGDEDDGAG
                     DWVRHAGGVVTDDATAPGDAARRLGEGTWPPEGAEPVDVGALYDRLADVGFGYGPAFQ
                     GVVAAWRREDETFCELALGEESAGDAAGFGVHPALLDASFHAVLDGLSAELGGGSLPL
                     PFALSGVRVHRAGTTSLRVALTTAADGSTVSLAAVDESGQPVLELESLAMQPVDGQRL
                     GATTRTGSDSLFRHAWVEVALDGADAGSETYAVLGGGADLPDDSPVHADLDALIAANE
                     DGAAEHDVVFASAADIAFAAAPDGDGAAAVHAGVVGLLSFLQSWLAAETESRLVLVTR
                     GAMGVAHGEAPDPARAALWGLVRSAQSEHPGRFVLVDLGEREDASAIDWPALLAADEP
                     QLAVRRGTAYALRVARLHSGKALEAPAGADAWHLDAPQRGTLENLTLVASERAGKPLG
                     AEEVRIAMRAGGLNFRDVLIALGQYPDDDPIGSEGAGVVLEVGEAVTDLAAGDRVFGL
                     IPEALGPVAVTDRRLVARAPDDWSFAEAASVPIVFMTAYYGLVDLGRLSEGETVLVHA
                     GAGGVGMAAIQIARHLGAEVYATASPGKWDVLRGLGLDDDRIASSRDLEFRERFLAAT
                     GDKGVDVVLNALAREFVDASLDLLPRGGRFVEMGKADVRDADQVARDHAGVEYTAYDL
                     VRNAGPDRIAELLGEIVALFESGALSHLPLRALDVRHAASAFRHLGDGRNVGKVVLTI
                     PPSLDPDGTVLITGGTGDLGARVARHLAGEHGVRHLLLVSRRGQDSPGAAELTEELRG
                     LGAEPSIAACDVTDRDDLTALLAGIGAEHPLTAVVHTAGVLDDGVIESLTPEQVQRVL
                     QPKVDAGLLLHELTLGTDLAEFILFSSDSGTVGVPGQGNYAAANVFLDALAERRAAQG
                     LAGKSLAWGLWSNATGMAGNLGEADIARLKRLGVAALSDELTLFDGARASAESVVVPT
                     RLDMPALRASAEAGTLAPLMRDLVRGRRRRERDTGVSLEQQLAGVPEEDREAAVLDVV
                     SAQIAVVLGHDSADAVDSERKFKELGFDSLSAVELRNRLAQVSGMRLPSTLIFDHPSP
                     IAVARYLLSTLTPEPATDGEPSGQASEAEIRKVIASIPVEQLRSAGLLDPLVRLAGGN
                     GGETEPEPDEDRSDSVDELDVDELVRMVDGP"
     gene            7987..18786
                     /locus_tag="VF517_06485"
     CDS             7987..18786
                     /locus_tag="VF517_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8052621.1"
                     /translation="MSATHEEIVAALRKSVKEREQLREQNRRLAAKDREPIAIVGMGC
                     RFPGGVRSPEDLWRLVTSEVDAISEFPSDRGWDLDRLYDADPDARGAAYTRHGGFIDS
                     AAEFDAEFFGISPREALAIDPQQRVLLEVAWDAIESAGIDPASLSGTPAGVFAGAGAT
                     EYAGRVAGDFEGYRLTGTHTSVISGRIAYTLGIEGPAVTVDTACSSSLVAMHLACGAL
                     RRGEAPLALAGGVTIMSEPWMLVEFSRQRGLAPDGRCKSFAESADGTGLAEGAGMLVL
                     ERLSDARRNGHRVLAVIRGSAVNQDGASNGLTAPNGPSQERVIQEALASADLEPAEVD
                     AVEAHGTGTTLGDPIEAQALLATYCKGRAGNPLRLGSVKSNIGHMQAAAGVGGVIKMV
                     MALRNGQLPRTLHVDEPSSHVDWSAGDIRLLTEAEPWERGEQPRRAGVSSFGISGTNA
                     HTIIEEAPEVEEPAAVAPDPAEPPTVAWLVSAKSEPALRAQAQQLREYVREHDELTPV
                     DVAFSLAVGRGRYERRAAVVGADRDSLLAGLEALERGEQPASGVTGAVRGGRTAFLFT
                     GQGAQRAAMGRELAAAFPVFAEALDAVCAEIDQHIDRPLRELLFADPGSDDAALLDRT
                     EFTQIALFALEVALFRLVESLGVKPDFLAGHSVGELSAAHVAGVLSLPDACALVAARG
                     RLIGELPDGGGMLAVKATEDEVRESLAGFEGRLSIAAVNGPSSVVVSGEAGALDELEP
                     VWTERERDTKRLRVSHAFHSPLVEPMLDEFRKVAEGLTFSAPRIPIVSSLTGERAAEQ
                     EITSADYWVRHVREAVRFADVVATLDGAGVRRFLELGPDGVLLGMAAEHLGAEADERA
                     LLVPALRSKRGDAEAFATFLAAADAAGVAIDWPAFFAERGARRVDLPTYAFQRERYWL
                     ESRAGAEAGAGHPSLSGHPILTNMAALAREDEWLFMGRLSLQANEWIASHVMSDTVVL
                     PGTAFVDMVLQAGAAIGCDLVEELTLESALLPPDHGDVEVQVFVGGADESGSRQFTIH
                     SRATEDADWIANASGTIAAAATEEDPLLDPLAAEEWPPAGAEEVEPDWVVERLGEIAG
                     FDYGPAFIGVTSVWRRGDAILSEVEIADDYLAESARYAVHPALLDMALHAGFAQYADD
                     TDLPPGQGKMLFRWANTRLHSTGATRLRVRAAPAGPDAISVAAFTEDGTPVLSVEALA
                     ARSVDVEQVQASLRGERRSLYRVEWPALGDPAAAGGELPPAAVVGDADADGIENRHAD
                     LAALVDSLEPGAPAPEVVIAGIAPGGGDVAARTGAAVAEALELLQAWIAAARLADSRL
                     VIVTRGAVAAAEGEVPDPALAAVWGLVRSAQSEHPGSFLLVDRDAEGEPPWAQLLATG
                     EPQLAVRGGELRTPRLAAVTQAGGEPVSWESEGTVLLTGGTGGIGALLARHLADRGAR
                     HLTLVSRRGPDAEGAGELVADLGERGCEAQVVACDVADRDACAELVESITADRALAAV
                     IHVAGVLDDATIESLTTEQLERVMRVKVAAASHLYELTEGLDLTAFVSFSSVAAVLGA
                     PGQGNYAAANSFLDALAQSGRAAGRSLTSLAWGPWDRDSGMTATIGAADIARMERMGI
                     GSVTPEVGLELFDAALDSGDSALVPVRLDSGALRTQARDGTLPAILSGLVAAPARRAG
                     AGGGSLARRLDELPEERWDEAVLDLVRKQVAAVLGHDSPDAVEPARSFKELGFDSLSA
                     VELRNRLIQATGLRLPSTLVFDHPTLEAVAKLVRSEVSGAERGSRAKSRARTRSDEPI
                     AIVGMSCRFPGGVSSPEELWELVASGTDAISPFPDNRGWDLDRLFDPDPDEPGTTYTR
                     EGGFIHDAPDFDAGFFGIGRSEALAMDPQQRLMLEGAWDAFQSAGIDPASLRDTETGV
                     FAGATVSDYRKSIGGEYEGFRLTGTTMSVLSGRLAHTFGLQGPAMTVDTACSSSAVAL
                     HLACQALRNAECSLALAGGVTVMGSPYLLIDFARQRGLAPDGRCKSFGAGADGVGFSD
                     GLGVLVLERLSDARRNGHRVLALIRGSAVNQDGASNGLTAPNGPSQERVIHQALESAG
                     LSPADVDAVDGHGTGTTLGDPIEAQALLATYGQERADGPLRLGSIKSNIGHTSAAAGV
                     AGVIKMVMALRHGELPRSLNCEEPSPHVDWSAGEVRLLAEPEPWEPGERPRRAGVSSF
                     GISGTNVHLIVEEAPAEEAPAEEPTAELPVLPIVVSARSEAALRGQAERLRGWITERV
                     ELEPRDVAFSLASARAQLDRRAVVLGSDREELAAGLDALARDGDEGAVVGQARSGKSV
                     FVFPGQGSQWEGMALELLDAAPVFAESLRECGEALSRYVDWSLEDVLRGADGAPSLER
                     VDVVQPALFAVMVSLAALWRSFGVEPSAVVGHSQGEIAAAYVAGGLSLDDAARVVCLR
                     SQAVADELAGHGGMGSIALSPEDAEAKLEPYGERLSLAAVNGPASVVISGEMEALDEL
                     LAACEADGTWARKIPVDYPSHSKAVERIEARLAEDLGPIEPKTGSVPFFSTVEAEVVD
                     TATLDAGYWYRSLRNRVRFNDAVAALIEDGASAFLEMSPHPGLTVGVAAAADAAGASE
                     RIAAVGSLRRGEGGLERFLASLAEAHVHGVDVNWSQLFSGTGARVVDLPTYAFQRERY
                     WLEGDGGAGDLAAAGLMAVDHPLLSTGQLLAGTDEWLFTGRASRATQTWISDHVLLDT
                     VVVPGTAWVEVAMAAGAAVDCEMLDELTFESPLVLGENEGAQLQVRVDGADPSGRREF
                     AIYSRPEDPSREGDDQEWTRHASGAVASAAEAERSEQIERLAAEAWPPEGAEAIDVDG
                     LYDRLVGMGFAYGSAFMGVEAAWRRGDELFAEVALDGEHAAEAERYGMHPALFDTAMH
                     PVVALLAERDDGAGRMLFHWKGMRRYSGQVTSMRVRLELVSDEAWNVAALDVFGADVL
                     SVDAVVARPVEAAQLALARRDHDDARLRLDWVEVPASSTNGHKPLFAQLAGVDADGLG
                     ESYADLPALVEAIEGGAQAPDVVLAAMPRVDAGDAAESARAGACQALELVQGWLADER
                     LSGARCVLVTRDGVAVSGDESPDLAAAALWGLVRSAQSEHPGRFGLLDIDGADASWQA
                     AAALLAAGEPQLALRDGVARVPRIARATAAPAATAPAFDPDGTVLITGGTGGLGALVA
                     RHLAAEHGVRHVLLASRRGTEAPEWAQLEAELAELDVEPVVAACDVADRGQLEALIAS
                     VAPEHPLTSVIHTAGVIEDGLVESLTPEQVDRVMRPKVDGALHLHELTEGLELAQFVI
                     FSSFAATLGSPGQANYAAGNAFADALAQRRHASGLAAKSLVWGAWAEAGGMTRDRSEA
                     DAARIRRLGAALLTEAEGLALFDAALDADDELPVLVRLESGTLRGMARDGVLPPILSG
                     LVRTRPTRSGEAADSLQRRLSGVPEADWPETVLDLVRDQIAAVLGLESRDEVEPQVAF
                     KEMGFDSLAGVELRNRLLRSTRLQLPATLVFDHPTPASVAEYMLGQLPKGGGGGAERP
                     PIDGEFDRIERLVQEIATDERTRAQVETRLRAFNARIQSFLVDPGESADADLDEDLEA
                     VSDEEMFALIDKELGPS"
     gene            18783..25565
                     /locus_tag="VF517_06490"
     CDS             18783..25565
                     /locus_tag="VF517_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX8052622.1"
                     /translation="MTDDQRLRQYLRKVTGELRTAHRRVRELEQRDHEPIAIVGMSCR
                     YPQAVQSPEELWEVVAGGVDAVTEYPDDRGWDLERLYDPDPDNPGTVYSREGGFVRDV
                     ADFDAEFFGISPREALAMNPQQRITLEIAWEALEDAGIDPTSLHGSQTGVFAGVFHDD
                     YSVGPVRAMAAPELEGYAYMGDAACILSGRVSYTLGLEGPALSVDTACSSSLVAIHLA
                     SEALRNRDCSLALAGGVTTMSTPMFLISLSRQRAMSPDGRCRAFGAEANGVGFSEGAG
                     MLVLERLSDARRLGHRVLALVRGSAVNQDGASNGLSAPNGPSQERVIRQALASGGLTP
                     ADVDAVEGHGTGTTLGDPIEAQALLATYGQERENGPLRLGSIKSNIGHTSAAAGVAGV
                     IKMVMAMRHGVLPRSLYCEEPSPHVDWSMGDVRLLAEAEPWEPGSRPRRAGVSSFGIS
                     GTNAHVIVEEAPAAQPARSAPADGPAEAAAAAEPVAAEEAPVEGAPAAEAAPAEGAPA
                     NSPAEGAPAEGAPVDSPAEGAPAEPAAAPAPLPAIPLILSARTDAALAAQAERLRARL
                     LDRTELEPLDVAYSLATGRARFERRAALVAADRDELIACLDALAAGELPASGSVGEPR
                     SGKSAFLFSGQGAQWAGMGRDLYSSYPVFAQALDAACAELDAQLGRSLRELVFATEGS
                     PEAELLGRTENTQAALFALEVALYRLFESFGLRPDYLVGHSIGELSAAHVAGVLSLAD
                     ACVLVAARGRLMGALPEGGAMLAVEADEDELAPTLGEQLSIAAINGPRALVVSGERSA
                     VEQCEAHWRAQGRKTSRLDVSHAFHSLLMEPMLDEFRAVAAGLGFEPPQMAVVSNLTG
                     ELATDELTDPGYWVRHVREAVRFADGVRELERRGVTRFIELGPDGVLTAMARGCVSED
                     HAERSLFAAAMRAEREGPRTLAACAGAMEVAGLGLDWHAFFANRGAQRVDLPTYAFQR
                     ERYWLEPSESAGDLTAAGQASAEHPLLGAAVRLAGDQEGWLFTGRLSLRTHPWLADHA
                     VADVVILPGAAFMELALAVADRVGAGELEDLTLVAPLVLTQDASLAVQITVAEADEEG
                     RRPINVYSSAQGEEPGDEDWVLHASGLLGAEGAAASETDAALADFAAASWPPAGAEPV
                     DVETFYERVAAAGYDYGPNFQGMRAAYRKDGEWYAEVALAGEQETEAAGYGVHPALTD
                     AALHTIVLEALERLEPGAAPEVPFSFSGVRLLRHGASSLRVRMRTTQDEDSDARTVRL
                     LALDESGAPALAIEALKARAVDQASLKARASAGNDSLFDVEWVEVPAADTNGSTLNAT
                     VLGDEELDLDSPAVELTRHPDLDALAEAIAAGAPAPAVVLVRAASIAPADGGLADSVH
                     DLGARMLDLLQSWLASEPLAESRLVLLTDGALAVSGGESPNLAQAALLGLMRSAHSEN
                     PGRFGVIDLDGSSTAGLYGALVSGEPELALREGVARAPRLARAGSGTPLMPPEGEAAW
                     QLGIETPGTLESLVLIPSSTAREPLEPGQVRVAMHAAGLNFRDVVVALGLVSHAGGEL
                     GGEGAGVVSEVGPGVEGLAPGDRVMGLMTAALGTTTITDARLLAKIPDGWSFAEAASV
                     PIVFMTAYHGLFDLADLKQGEAVLLHGAAGGVGMAAIQLAAHVGAEVFATAHPSKWET
                     VEGLGVDRSHISSSRTLDFKDEVLERTGGRGVDVVLDSLAGEFVDASLDLLPRGGRFI
                     EIGKTDIRDADEVAAQHAGVAYQAFDLLEAGPGRIQTMLSEVVSLFEQGVLHHLPVST
                     WDLRRGPDAFRHMREAKHVGKIVLCVPQPPERDGTVLITGGTGGLGALVARHLAGEHG
                     ARRLVLTSRRGLEAEGAEELVAALAELGCEAEVAACDIADRQQVEALLAAIPAEHPLT
                     AIVHAAGVLDDGVIDSLDGERLRKVMAPKVDGALNLHELTEDAVLSQFVMFSSVASTL
                     GSPGQGNYAAANAFMEALAQHRQARGLPATALAWGAWERVGGMLGALSDADRSRGERM
                     GITALPDEQGLALLDTARAAAQPLLVPARLDSGALRSQAKAGVLPAILQGLVRAPVRR
                     AAETKGSLATRLAAAPESDWQAIVLDLVKSNVGGVLGHNSGDAIDPKRAFKDLGFDSL
                     SAVELRNRLTQATGMRLPTTLVFDHPSPAAVAEQLIGMAVPKAKTNGDSGESELREVL
                     ASIPIRRLREAGLLDPLVELARSDGSSSPSDGDGDGGAPAASIDEMDAEALIRMTRGE
                     VEESGLENALDS"
     gene            25574..>29739
                     /locus_tag="VF517_06495"
     CDS             25574..>29739
                     /locus_tag="VF517_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8052623.1"
                     /translation="MADNQEQLVEALRTSLKETERLRQQNRRLVAKATEPIAIVGMSC
                     RFPGGADSPAALWELVAEGRDAIGEFPGDRGWDLDHIYDPDPDALGTSYTRHGGFLAD
                     AAEFDADFFSISPREALAMDPQQRLLLEGAWQAFEDAGMAPTSLRGSATGVFVGVVNS
                     NYGIELESPKEVEGFRLTGTTTSVASGRLAYTLGLEGPAVSVDTACSSSLVALHLACQ
                     ALRRGECELALAGGATVLASPGLFIEFSRQRGLSRDGRCKAFGAGADGTGWSEGTGLF
                     VLERLSQARANGHEVLALVRGSAVNQDGASNGLTAPNGPSQERVIRQALESAGLSPDD
                     VDAVEGHGTGTTLGDPIEAQALLETYGRERSNGPLYLGSIKSNIGHSAAAAGAGAVVK
                     MVQALRHGLLPRTLHAEEPTPHVDWSAGAVELLQEPVEWPAGERVRRVGISSFGVSGT
                     NAHVILEEAPRVEAKAAAAPEADGDAPAVVREGALPFLVSAATPQALGEQAGRLAGFL
                     ERRPELDSYAVAAALATRRAQLPQRAVAVAGDHAELVASLRALERGELANGVVRGSAA
                     GGGATAFLFSGQGSQWAGMGSVLHREFPVFAAALDEVCAELDRHLERPLLEVMFAAEG
                     SEEAELLADTRFTQVALFALEVALARLVASFGIVPDYLIGHSIGEFSAAHVAGVFSLE
                     DGARLVAERARLMGALPAGGAMLAVEASEAEALEGLAGFEDRLALAAINAPTAAVVSG
                     EEQAIAEVEALWRARDRRTTRLRVSHAFHSQLMDPMLDDLRAVAAEIELSAPRIPIAS
                     NLTGEQLTADEATSPDYWVRHLRATVRFAAGVEHLRANGVTRFLELGPDGTLSAMAAQ
                     TAGAEEDEGEEREPLFVSALRGRKGPQREALLGFLAAAHCDGAELDWSALLDDRGVGR
                     LELPTYAFQRSRYWLVAGASAGDLTAAGQTSAEHPLLGAAVRLAGEGEGWLFTGRLSL
                     ADQPWLADHAVADVVILPGAAFVELALAVAARTGAGALEELTLVAPLVLAEDAALALQ
                     ITVAEPDEEGRQPIAVYSAPQAAAAASAAVGTEPGAAEPAADWTLHAQGQLGVGGADA
                     PESVTDAELAELAAAPWPPEGADSVDVSTFYERVAAAGYDYGPAFQGLRRAYRASSPD
                     GDEWFAEVALASDQETQAGSYGVHPALSDAALHTIVLEALERLEPGAAPEIPFSFSGV
                     RLQRPGAASLRVAMRTSEQEGSDARTVRILALDESGAPAFAIEALEARAVDQAALKAR
                     ASTGSDALFGLNWTEVQGAGADSTAPSAALVGSDDSGALDGASFELARYPDLEALAEA
                     IAAGAEAPQVVLARAVALAAPVIDAPTSDGSSASLAGSVHDLTARTLALLQSWLAAEP
                     LAESRLVLLT"
BASE COUNT         3646 a        10935 c        11305 g         3853 t
ORIGIN      
        1 cgggtcctcc gcgagcctcg ccggctcggt ccacgacctc accgcgcgca cgctcgcgct
       61 gctccagtcc tggctcgccg ccgagccgct cgccgagtcc cgcctcgtgc tcctgaccga
      121 caacgccctg gcgacctccg acgccgagtc gccgaacctc tcccagtccg ccctgctcgg
      181 cctcctgcgc agcgcccact ccgagaaccc gggacgcttc ggcgtgatcg acctcgacgg
      241 cgccgacgcc tcggcctccg cgctcgccgg cgcgctggcc gccgaggagc cggagctcgc
      301 cctgcgcgag ggccgcgcgc tcgcgccgcg gctggcgcgg gcgagcgtgg aggacgacgc
      361 ggcgcaggcc ggcgcggaag cgggcggcgg cgcgggcgcc gccggcgcag ggccggaccc
      421 cgatggcacg gtcctgatca ccggcggcac cggcgggctc ggagcgctca tcgcgggcca
      481 cctggtggcc gagcacggcg cgcggcggct gctcttgacg agccggcgcg gcctcgaagc
      541 cgacggtgcc aaggagctgg tcgcctcgct gaaggagctc ggctgcgagg cagaggtggc
      601 cgcgtgcgac gtcgccgacc gcgagcaggt cgaggcgctg cttgccgccg tccccgccga
      661 gcatccgctc acctcgatct accacgcggc gggcacgctc gacgacggcg tgatcgactc
      721 gctcgacgcc gagcggctcc agaaggtcat ggccccgaag gtcgacggtg cgctcaacct
      781 gcacgacctc accgagggcg ccgagctgtc cgagttcgtc ctgttctcgt cggtcgcctc
      841 caccctcggc actccgggcc agggcaacta cgcggcggcc aacgcgttcc tcgacgggct
      901 cgcccaccgg cgccgctcgc agggcctcgc cgccacgtcg ctggcctggg ggccgtgggc
      961 gcgcgccggc ggcatgctgg gcagcctcaa cgaggtggac ctggagcgct gggagcgcgc
     1021 cggcatctcc acgctctccg acgagcaggg ccttgcgatg ctcgacgcgg cccgcgccac
     1081 ggcgcagccg ctgctcgtgc ccgtggccct ggagggctcc gcgctccgcg cgcaggcgaa
     1141 ggccggcacg ctgccggcga tcttcagcgg gctgtaccgc acggcggtcc gccagtccgc
     1201 cgagggcggc ggctcgctgg cgacgcgact ggccgcgtcg ccggagtccg agtggcaggc
     1261 gatcgtcctc gagatcgtcc agacccacgt cgccggcgtg ctggggcaca gctcgggcga
     1321 ggccgtcgac ccgcagcgcg ccttcaagga gctgggcttc gactcgctcg gcgccgtgga
     1381 gctgcgcaac cgcctgggcc aggcgaccgg cctgaagctc ccgaccacgc tgatcttcga
     1441 ccacgccagc ccggccgcgg tcgcggagtt cctgcgttcg aaggtcggcg gcgcagagcg
     1501 cgccaagaag gtcacccgcg gctcgcgcac gcgcaacgac gagccgatcg ccatcgtcgg
     1561 catgagcgcc cgctacccag gcggcgtccg ctcgcccgac gacctctggc agctgatcgt
     1621 cgccggcggc gacgccatcg gcgagttccc cgaggaccgc ggctgggacc tcgagaagct
     1681 catccacccc gaccccgacc atccgggcac gagctacacg cgccacggcg gcttcgtcta
     1741 cgacgccgac cgcttcgacg ccgagttctt ctcgatctcc ccgcgagagg cgctcgcgct
     1801 ggatcctcag cagcgactgg tgctcgaggg cgcctgggag gcgctcgaaa acgcccggat
     1861 cgatccgctc agcctcaagg gcagccagac cggcgtcttc accggcgtca tgtaccacga
     1921 ctacggcatc gcggggatgc cgcccgagct cgagggcttt ctcggcccgg gcggcgccgg
     1981 cggcggcgcg atcctgtccg gccggctcgc ctacacgctc gggctcgagg gcccggcggt
     2041 ctcgatcgac acggcctgct cgtcctcgct cgtggctctc cacgtggccc gccaggcgct
     2101 cgagcagggc gagtgcgacc tcgcgctcgc cggcggggtg accgtgctct cgacgccgat
     2161 cgcgttcgtc cagttctcgc gccagcgcgc gctctcgccg gacgggcgct gccgggcgtt
     2221 cggcgccgac gccgacggcg tcggctgggc cgagggcgcg gggctactcg tgctcgagcg
     2281 gctctccgac gcgcgcgcga acgggcacga ggtgctcgcg ctggtccgcg gcagcgccgt
     2341 caaccaggac ggcgcctcga acgggctgac cgcgccgaac gggccctcgc aggagcgcgt
     2401 gatccgccag gcgctggcga gcgcgggcct gtcgccgtcc gacgtggacg tggtcgaggc
     2461 tcacggcacc ggcacgagtc tcggcgatcc gatcgaggcg caggcactgc tcgcgaccta
     2521 cgggcagaag cgttccaacg gtccgctcta cctcgggtcc gtgaagtcga acatcggaca
     2581 cacgcaggcg gcggcggggg tcgccggcgt gatcaagatg gtggaggcga tgcggcacgg
     2641 gcttctgccg cgctcgctct actgcgagga gccctcgccg cacgtggact ggtcggcggg
     2701 cgacatcgag ctgctcgcgt ggccggtgga ctggtcgccg ggcgagcgcg tccgccgcgc
     2761 cggcgtctcg tctttcggcg tgtccggcac caacgcgcac gtgatcgtgg aggaggcacc
     2821 cgcgccggag ccggagccgc tggtggaggc caacacgcac ctgcccgtgc tgccgatcgt
     2881 cgtctccgcg cgcagcgagg ccgccctgcg cggccaggcc gagcgcgtgc gcgagtggct
     2941 ctacgagcgc gtcgagctgg agccgatcga cgtcgctttt tcgcttgcca gccaccgtgc
     3001 gcagctcgac cgccgcgcgg tggtgctcgg ctccgaccgc gccgagctgg tggcggggct
     3061 cgaggctctg gcccgtgacg aaacagacgc cgccgtggtc ggccaggcgc gcaccggcaa
     3121 gtcggtgttc gtcttcccgg ggcagggctc gcagtgggag ggtatggcgc tcgagctgct
     3181 cgacttggcg ccggccttcg ccgagtcgct gcgcgcgtgc ggcgaggcgc ttgccaaata
     3241 cgtcgactgg tcgctcgagg acgtgctgcg cggggcggaa ggtgccccgt cgctcgaccg
     3301 cgtcgacgtg gtgcagcccg cgctgttcgc ggtgatggtc tcgctggccg ctctgtggcg
     3361 ctcatacggc gtcgagccgt cggccgtggt cggccactcg cagggcgaga tcgccgcggc
     3421 ccacgtcgcc ggcggcctga gcctcgatga cgccgcacgc gtcgtctgcc tgcgcagcca
     3481 ggcggtgctc gacgagctgg ccgggaaggg cggcatgggc tcggtggcgc tgtcgccgga
     3541 ggacgcggag accaggttgc agccctacgg cgagcgcctc tcgctggccg ccgtcaacgg
     3601 ccccgcctcg gtcgtgatct ccggcgagat ggaggcgctc gacgagctgc tggcggcctg
     3661 cgaggccgat ggcacctggg cgcgcaagat cccggtggac tacccctcgc actccaaggc
     3721 ggtcgagcgg atcgagcagc gactggccga ggagctcggc ccgatcgagc ccaagacggg
     3781 cagcgtgccg ttcttctcga ccgtcgaagc cgaggtggtc gacaccgcga cgctcgacgc
     3841 cggctactgg tatcgctcgc tgcgcaaccg cgtgcgcttc aacgacgccg tcgcgaccct
     3901 gatcgaggac ggcgccagcg ccttcctcga gatgagcccg caccccggcc tgacggtcgg
     3961 cgtggcggcc gccgccgacg ccgccggcgc cgccgagcgc atcgccgcgg tcggctcgct
     4021 gcgccgcggc gagggtgggc tcgagcgctt cctggcctcg ctcgccgagg cgcacgtgca
     4081 cggcgtcgac gtgaactggt cgcagctctt cgccggcacc ggcgcgcgcg tggtcgacct
     4141 gccgacctac gcgttccagc gcgagcgcta ctggctcgag gcgggcacga acctcgggga
     4201 cctggcggcg gcggggctcg ggtcggtgga ccacccgctt ctgagcgcga tgctggcgag
     4261 tcccggcaat cgcggcgcgt cgttcaccgg gcgggtgtcg ctcgcggcgc agccgtggct
     4321 ggccgaccac gcggtcttcg atcgcgtgct gttcccgggg acggcgttcg ctgagctgct
     4381 gctggccgca ggcgccgagc tgggctgccc ggtggtcgag gagctgacgc tcgaggcgcc
     4441 gctgctcctg gccgagggcg acgaggtggc actccaggtc gtcgtgggcg aggacgacgg
     4501 tcgcgggcgc cgccaggtcg aggtgtattc caagccgccc gcacacgccg agcacgagga
     4561 cgacggcgac gaggacgacg gcgccggcga ctgggtccgc cacgccggcg gcgtggtcac
     4621 cgacgacgcg accgcccccg gcgacgccgc gcgcaggctc ggcgagggga cgtggccgcc
     4681 ggagggcgcc gagccggtcg acgtgggcgc gctctacgac cgcctcgcgg acgtcggttt
     4741 cggctacggc ccggcgttcc agggggtcgt ggcggcgtgg cggcgcgagg acgagacctt
     4801 ctgcgagctg gcgttgggcg aggagtcggc cggcgacgcc gccgggttcg gcgtgcaccc
     4861 ggcgctgctc gacgcctcct tccacgctgt gctcgacggg ctgtccgcgg agctgggcgg
     4921 cggcagcctg ccgctgccgt tcgcgctcag cggcgtgcgc gtgcaccgcg ccgggaccac
     4981 gtcgctgcgg gtcgcgctga cgaccgccgc cgacgggagc acggtgagcc ttgccgcggt
     5041 ggacgagagc ggccagccgg tgctggagct cgagtcgctg gcgatgcagc cggtcgacgg
     5101 tcagcgcctc ggcgcgacca cgcgcacggg cagcgattcg ctcttcaggc acgcgtgggt
     5161 cgaggtcgcc ctcgacggcg ccgacgcggg gtcggagacc tacgcggtgc tcggcggcgg
     5221 cgccgacctg ccggacgact cgccggtcca cgccgacctc gacgcgctga tagcggccaa
     5281 cgaggatggc gccgccgagc acgacgtcgt gttcgcctcc gccgccgaca tcgcgttcgc
     5341 cgccgcgccg gacggcgacg gcgccgccgc ggtgcacgcg ggcgtggtcg gcctgctgtc
     5401 gttcctccag tcctggctgg cggcggagac ggagtcgcgc ctggtgctcg taacgcgcgg
     5461 tgcgatgggc gtggcgcacg gcgaggcgcc cgatcccgcc cgcgcggcgc tctggggcct
     5521 cgtccgcagc gcccagtccg agcacccggg ccgcttcgtg ctcgtggacc tcggcgagcg
     5581 cgaggacgcc tcggcgatcg actggcccgc gctgctggcc gccgacgagc cccagctcgc
     5641 cgtgcgccgc ggcaccgcgt acgcgctgcg cgtcgcccgc ctccacagcg gcaaggccct
     5701 ggaggcgccc gccggcgccg acgcgtggca cctcgacgcg ccgcagcggg gaacgctcga
     5761 gaacctcacc ctggtcgcga gcgagcgcgc cgggaagccg ctgggcgccg aggaggtccg
     5821 gatcgcgatg cgcgcgggcg gcctcaactt ccgcgacgtg ctgatcgcgc tcgggcagta
     5881 cccggacgac gacccgatcg gcagcgaggg cgccggtgtg gtgctcgagg tgggcgaggc
     5941 ggtgacggac ctcgcagccg gcgaccgcgt gttcggcttg atccccgagg cactcggccc
     6001 ggtggcggtg accgatcgcc ggctcgtggc ccgcgcgccg gacgactggt cgttcgccga
     6061 ggcggcctcc gtgccgatcg tgttcatgac cgcgtactac ggcctggtcg acctcggccg
     6121 gctgagcgag ggcgagaccg tgctcgtgca cgcaggcgcc ggcggcgtgg gaatggcggc
     6181 gatccagatc gcgcgccacc tgggcgccga ggtgtacgcc acggcgagcc ccggcaagtg
     6241 ggacgtcctg cgcgggctcg gcctcgacga cgatcgcatc gcgtcctcgc gcgacctcga
     6301 gttccgcgag cgcttcctgg cggcgaccgg cgacaagggc gtggacgtgg tgctgaacgc
     6361 gctggcccgc gagttcgtcg acgcctcgct cgacctcctc ccgcgcggcg gccgcttcgt
     6421 cgagatgggc aaggccgacg tgcgcgacgc cgaccaggtc gcacgcgacc acgcgggcgt
     6481 cgagtacacc gcctacgacc tggtgcgcaa cgccggcccg gaccgcatcg ccgagctgct
     6541 cggcgagatc gtcgcgctgt tcgagtcggg tgcgctgagc cacctgccgc tgcgcgcgct
     6601 ggacgtgcgc cacgccgcct ccgcgttccg ccacctcggc gacgggcgca acgtcggcaa
     6661 ggtcgtgctc acgatcccgc cgtcgctcga ccccgacggc acggtcctga tcaccggcgg
     6721 cacgggcgac ctgggcgcac gcgtggcgcg ccacctcgcg ggcgagcacg gcgtgcgcca
     6781 cctgctgctc gtgagccggc gcgggcagga ctcgccgggc gccgcggagc tgaccgagga
     6841 gctgcgcggg ctgggcgccg agccgagcat cgcggcctgc gacgtgaccg accgcgacga
     6901 cctaacggcc ctgctcgccg gtatcggcgc cgagcacccg ctgacggcgg tggtccacac
     6961 ggcgggcgtg ctcgacgacg gcgtgatcga gtcgctcacc cccgagcagg tccagcgcgt
     7021 gctccagccg aaggtcgacg cggggctgct cctgcacgag ctgacgctcg gcaccgacct
     7081 ggcggagttc atcctcttct cctccgactc gggcacggtc ggcgtgccgg gtcagggcaa
     7141 ctacgcggcg gccaacgtct tcctcgacgc gctcgccgag cgccgcgcgg cgcaggggct
     7201 ggcgggcaag tcgctcgcct ggggtctgtg gagcaacgcg accggtatgg cgggcaacct
     7261 cggcgaggcc gacatcgcgc gcctgaagcg cctcggcgtg gccgccctct cggacgagct
     7321 cacgctgttc gacggcgccc gcgcgagcgc ggagtcggtc gtcgtgccca cccggctcga
     7381 catgccggcc ctgcgcgcct cggccgaggc cggcacgctc gcgccgctca tgcgcgatct
     7441 cgtgcgcggg cggcgccgcc gcgagcgcga cacaggcgtc tcgctcgagc agcagctcgc
     7501 gggcgtgccc gaggaggacc gcgaggccgc ggtgctggac gtcgtcagcg cgcagatcgc
     7561 cgtcgtgctc ggccacgact cggcggacgc cgtcgattcc gagcgcaagt tcaaggagct
     7621 cggattcgat tcgctgagcg cggtcgagct gcgcaaccgc ctcgcgcagg tcagcggaat
     7681 gcggcttccg tccacgctga tcttcgacca cccgagcccg atcgcggtgg cccgctacct
     7741 gctgagcacg ctcacgccgg agccggcgac cgacggcgag ccaagcggcc aggcgagcga
     7801 ggccgagatc cgcaaggtga tcgcgtcgat cccggtggag cagctgcgca gcgccggcct
     7861 tctcgacccg ctcgtgcgcc tggccggggg caacggtggg gagaccgagc cggagccaga
     7921 cgaggatcgg tccgactccg tggacgaact ggacgtggac gagcttgtgc ggatggtgga
     7981 cgggccgtga gcgccaccca cgaagagatc gtcgcggcgc tcaggaagtc ggtcaaggag
     8041 cgcgagcagc tgcgcgagca gaaccgccgg ctcgcggcga aggatcgcga gccgatcgcc
     8101 atcgtcggca tgggctgccg cttcccgggc ggcgtgcgct cgccggagga cctctggcgg
     8161 ctcgtcacct cggaggtgga cgcgatttcg gagttcccgt ccgaccgcgg ctgggacctc
     8221 gaccgcctct acgacgccga cccggacgcc cgcggcgcgg cctacacccg gcacggcggc
     8281 ttcatcgaca gcgccgccga gttcgacgcc gagttcttcg ggatcagccc gcgcgaggcg
     8341 ctcgcgatcg acccgcagca gcgcgtgctg ctcgaggtcg cgtgggacgc gatcgagagc
     8401 gccggcatcg acccggcctc gctgtcgggc acacccgccg gcgtgttcgc gggagccggc
     8461 gcgaccgagt acgccgggcg cgtggcgggc gacttcgagg gctaccgcct gaccggcacg
     8521 cacaccagcg tcatctccgg ccgcatcgcg tacacgctcg gcatcgaggg cccggccgtg
     8581 accgtcgaca ccgcgtgctc gtcgtcgctc gtggcgatgc acctggcctg cggcgccctg
     8641 cggcgcggtg aggccccgct cgcgctcgcc ggcggcgtga cgatcatgtc cgagccgtgg
     8701 atgctggtgg agttcagtcg ccagcgcggc cttgcgcctg acgggcgctg caagtcgttc
     8761 gccgagagcg ccgacggcac cggcctggcc gagggcgccg gcatgctcgt cctcgagcgg
     8821 ctctcggacg cgcgccgcaa cggccaccgc gtgctggcgg tgatccgcgg cagcgcggtc
     8881 aaccaggacg gcgcgagcaa cgggctgacc gcgccgaacg ggccctcgca ggagcgcgtg
     8941 atccaggagg cgctggcgag cgcggacctc gagcccgccg aggtcgacgc cgtggaggcc
     9001 cacggcacgg gcaccacgct cggcgacccg atcgaggcgc aggcgctgct ggccacctac
     9061 tgcaagggac gcgcgggcaa cccgctccgc ctcggctcgg tcaagtcgaa catcggccac
     9121 atgcaggccg cggccggcgt gggcggcgtg atcaagatgg tgatggcgct gcgcaacggg
     9181 cagctccctc ggacgctgca cgtggacgag ccctcatccc acgtcgactg gagcgcgggc
     9241 gacatcaggc tgctgaccga ggccgagccc tgggagcgcg gcgagcagcc gcggcgcgcg
     9301 ggcgtgtcgt cgttcggcat cagcggcacc aacgcccaca cgatcatcga ggaggcgccc
     9361 gaggtcgagg agccggccgc cgtcgcgccg gatccggccg agccgccgac ggtcgcgtgg
     9421 ctcgtgtcgg ccaagagcga gcccgcgctg cgggcccagg cgcagcagct gcgcgaatac
     9481 gtgcgcgagc acgacgagct gacgcccgtg gacgtcgcgt tctcgctcgc ggtcggtcgc
     9541 ggccgctacg agcggcgcgc cgccgtggtc ggcgccgacc gggacagcct gctcgccggg
     9601 ctcgaggcgc tggaacgcgg cgagcagccg gccagcggcg tcacgggcgc ggtgcgcggc
     9661 ggcaggacgg cgttcctgtt caccgggcag ggcgcacagc gcgcggcgat gggacgcgag
     9721 ctcgcggctg cgttccccgt gttcgccgag gcgctggacg cggtctgcgc ggagatcgat
     9781 caacacatcg accgcccgct gcgcgagctg ctgttcgccg atccgggatc cgacgacgcc
     9841 gcgctcctcg accgcaccga gttcacccag atcgcgctgt tcgcgctcga ggtggcgctc
     9901 ttccggctgg tggagtccct cggggtgaag ccggacttcc tcgccgggca ctcggtcggc
     9961 gagctgtcgg ccgcgcacgt cgcgggcgtg ctgtcgctgc ccgacgcgtg cgcgctggtg
    10021 gcggccaggg ggaggctgat cggcgagctc ccggacgggg gcgggatgct cgccgtgaag
    10081 gccaccgagg acgaggtgag ggagtcgctc gccgggttcg agggcaggct gtcgatcgcg
    10141 gcggtgaacg ggccgagctc ggtcgtggtc tccggcgagg ccggtgcgct cgacgagctc
    10201 gagccggtgt ggaccgagcg cgagcgcgac accaagcgcc tgcgcgttag ccacgcgttc
    10261 cactcgccgc tggtcgagcc gatgctcgac gagttccgca aggtcgccga gggcctgacg
    10321 ttctcggcgc cccgcatccc gatcgtgtcg agcctcaccg gcgagcgcgc cgccgagcag
    10381 gagatcacct cggccgacta ctgggtccgc cacgtgcgcg aggcggtccg gttcgccgat
    10441 gtcgtcgcga cgctggacgg cgccggcgtg cggcgcttcc tcgagctcgg ccccgacggc
    10501 gtgctgctcg ggatggcggc cgagcacctc ggcgcggagg ccgacgagcg ggctctgctc
    10561 gtaccggctc tgcgctcgaa gcgcggcgac gccgaggcgt tcgcgacctt cctcgccgcg
    10621 gccgacgcgg ccggtgtcgc gatcgactgg ccggcgttct tcgccgagcg cggcgcgcgg
    10681 cgcgtggacc tgcccacgta cgcgttccag cgcgagcgct actggctcga gtcgcgcgcg
    10741 ggcgccgagg ccggcgcggg ccatccgagc ctgagcggcc acccgatcct cacgaacatg
    10801 gccgccctcg cccgcgagga cgagtggctg ttcatgggac gcctctcgct gcaggcgaac
    10861 gagtggatcg cgagccacgt gatgtccgac accgtcgtcc tcccgggcac cgcgttcgtg
    10921 gacatggtgc tccaggcggg agcggccatc ggctgcgacc tcgtcgagga gctcacgctc
    10981 gagtcggccc tcctgccgcc ggaccacggg gatgtcgagg tccaggtgtt cgtcggcggc
    11041 gcggacgagt ccgggagccg gcagttcacg atccactccc gcgcgacgga ggacgcggac
    11101 tggatcgcca acgcgagcgg gacgatcgcg gcggcggcga cggaggagga ccccctgctc
    11161 gatccgctgg cggcggagga gtggccgccg gccggcgcgg aggaggtcga gccggactgg
    11221 gtggtcgagc gcctcggcga gatcgccggc ttcgactacg gccccgcctt catcggggtg
    11281 acctcggtgt ggcgccgcgg cgacgcgatc ctctccgagg tcgagatcgc cgacgactac
    11341 ctcgccgagt ccgcgcgcta cgcggtgcat ccggcgctgc tcgacatggc gctgcacgcg
    11401 ggcttcgccc agtacgccga cgacacggac ctcccgccgg gccaggggaa gatgctgttc
    11461 cgctgggcca acacccgcct gcactccacc ggcgcgacgc ggctacgcgt gcgcgccgcc
    11521 ccggccggcc ccgacgcgat ctcggtcgcc gcgttcaccg aggacggcac tccggtgttg
    11581 tcggtcgagg cactggccgc gcggtccgtg gacgtcgagc aggtccaggc atcgctgagg
    11641 ggcgagcgcc gttccctgta tcgcgtcgag tggcccgcgc tgggcgaccc tgccgcggcg
    11701 ggcggcgagc tgccgccggc ggcggtcgtc ggcgacgccg acgcggacgg gatcgagaac
    11761 cgccacgcgg acctggccgc gctggtcgac tcgctcgaac cgggggcccc ggcgcccgag
    11821 gtcgtgatcg ccggcatcgc gccgggcggc ggcgacgtgg ccgcgcggac cggcgccgcc
    11881 gtggccgagg cgctcgagct gctccaagcg tggatcgccg cggcgcgcct ggccgactcg
    11941 cggctcgtga tcgtcacgcg cggcgcggtg gctgcggccg agggcgaagt gcccgacccg
    12001 gcgctcgccg cggtctgggg actcgtgcgc agcgcccagt ccgagcatcc cggctccttc
    12061 ctgctggtcg accgcgacgc cgagggcgag ccgccgtggg cgcagctcct cgcgaccggc
    12121 gagccgcagc tcgcggtgcg cggcggcgag ctgcgcacgc cgcggctggc cgcggtgacg
    12181 caggccggcg gggagccggt gtcgtgggag agcgaaggca ccgtgctcct caccggcggc
    12241 accgggggaa tcggcgcgct gctcgcgcgg cacctcgcgg atcgcggcgc ccggcacctg
    12301 acgctcgtca gccgtcgcgg accggacgcc gagggcgccg gcgagctggt cgccgacctc
    12361 ggcgagcgcg ggtgcgaggc gcaggtcgtc gcctgtgacg tcgccgaccg cgacgcctgc
    12421 gccgagctgg tcgagtcgat cacggccgac cgggccctcg cggcggtcat ccacgtcgcc
    12481 ggcgtgctcg acgacgcgac gatcgagtcg ctcacgaccg agcagctcga gcgggtcatg
    12541 cgcgtgaagg tcgccgctgc ctcgcacctg tacgagctga ccgaaggcct cgacctcacc
    12601 gcgttcgtct cgttctcgtc cgtggccgcc gtcctcggcg cgcccggcca gggcaactac
    12661 gcagccgcta actcgttcct cgacgcgctc gcgcagagcg gccgcgccgc cggccggtcg
    12721 ctcacatcgc tcgcgtgggg accgtgggat cgcgacagcg ggatgacggc gacgatcggc
    12781 gcggcggaca tcgcgcgcat ggagcgcatg ggcatcggct cggtgacccc cgaggtcggc
    12841 ctcgagctct tcgacgcggc gctcgactcc ggcgactcgg cgctggtgcc ggtgcgcctc
    12901 gacagcggcg cgctgcgcac gcaggcgcgc gacggcacgc tgcccgcgat cctgagcggg
    12961 ctggtggcgg cccccgcacg gcgcgcgggc gctggtggcg gatcgctcgc gcggcgactg
    13021 gacgagcttc cggaggagag gtgggacgag gccgtgctcg acctcgtccg caagcaggtt
    13081 gctgcggtgt tgggccatga ctcgcccgac gcggtggagc cagcgcgctc gttcaaggag
    13141 ctcgggttcg actcgctctc ggcggtcgag ctgcgcaacc ggctgatcca ggccaccggg
    13201 ctgcgcctgc cctcgacgct cgtgttcgac cacccgacgc tcgaggcggt ggcgaagctc
    13261 gtccgctcgg aggtctcggg cgccgagcgc ggcagcaggg cgaagtcgcg cgcacgtacg
    13321 cgcagcgacg agccgatcgc gatcgtcggg atgagctgcc ggttccccgg cggcgtgtcc
    13381 tcgccggagg agctgtggga gctcgtcgcc tccgggaccg acgcgatctc gccgttcccg
    13441 gacaaccgcg gctgggacct cgaccggctg ttcgatcccg acccagacga gcccggcacc
    13501 acatacacgc gcgagggcgg cttcatccac gacgcgccgg atttcgacgc cgggttcttc
    13561 gggatcgggc gcagcgaggc cctggccatg gacccccagc agcggctgat gctggaggga
    13621 gcctgggatg cgttccagag cgcgggcatc gaccccgcct cgctgcgcga caccgagacg
    13681 ggcgtgttcg ccggcgcgac ggtctccgac taccgcaaga gcatcggcgg ggagtacgag
    13741 ggcttccggc tgaccggcac cacgatgagc gtcctgtccg gccggctcgc ccacacgttc
    13801 ggcctccagg gccccgcgat gaccgtggac acggcgtgct cgtcgtcggc ggtggcgctg
    13861 cacctcgcgt gccaggcgct gcggaacgcg gagtgctccc tggcgctcgc tggtggcgtg
    13921 accgtgatgg ggtcgcccta cctgctgatc gacttcgcgc gtcagcgtgg cctcgccccg
    13981 gacgggcgct gcaagtcgtt cggcgcaggc gcggacggcg tcggattctc cgacggcctc
    14041 ggcgtgctcg tgctggagcg gctgtccgac gcgcgccgca acggccaccg cgtgctggct
    14101 ctgatccgcg gcagcgcggt gaaccaggac ggcgcctcga acggcctcac ggcgccgaac
    14161 gggccctcgc aggagcgcgt gatccaccag gcgctggaga gcgcggggct ctcgcccgcc
    14221 gacgtggacg cggtggacgg gcacgggacg ggcacgacgc tgggcgatcc gatcgaggcg
    14281 caggcgctgc tggcgaccta cggtcaggag cgcgccgacg gtccgctgcg gctcgggtcg
    14341 atcaagtcga acatcggtca cacctcggcg gcggccgggg tcgccggcgt gatcaagatg
    14401 gtgatggcgc tgcgccacgg cgagctgccg cgatcgctca actgcgagga gccctcgccg
    14461 cacgtcgact ggtcggcggg cgaggtgcgg ctgctggcgg agcccgagcc gtgggagccg
    14521 ggggagcggc cgcggcgagc gggcgtgtcg tcgttcggga tcagtgggac gaacgtgcac
    14581 ctgatcgtgg aggaggcccc cgcggaggag gcgccggccg aggagccgac cgccgagctg
    14641 cccgtgctgc cgatcgtcgt atccgcccgc agcgaggccg cattgcgcgg ccaggccgag
    14701 cgcctgcgcg ggtggatcac cgagcgcgtc gagctcgagc cgcgcgacgt cgctttctcg
    14761 ctggcgagcg cccgcgcgca gctcgaccgc cgcgcggtcg tgctcggctc agaccgcgag
    14821 gagctcgccg ccggcctcga cgcgctcgcc cgcgacggcg acgagggcgc cgtcgtcggc
    14881 caggcgcgct ccggcaagtc ggtcttcgtc ttcccgggcc agggatcgca gtgggagggc
    14941 atggcgctcg agctgctcga cgccgcgccg gtcttcgcgg agtcgctgcg cgagtgcggc
    15001 gaggctctgt cgcgctacgt cgactggtcg ctcgaggacg ttctgcgcgg tgccgacggc
    15061 gccccgtcgc tcgaacgcgt ggacgtcgtc cagcccgccc tgttcgcggt gatggtctcg
    15121 ctcgcggcgc tgtggcgcag cttcggggtc gagccctcgg ccgtggtggg ccactcccag
    15181 ggcgagatcg ccgccgccta cgtcgccggc ggcctctcgc tcgacgacgc cgcccgcgtc
    15241 gtctgcctgc gcagccaggc cgtcgccgac gagctcgccg gccacggcgg gatgggctcg
    15301 attgcgctaa gccccgagga cgccgaggcg aagctcgagc cctacggcga gcgcctctcg
    15361 ctcgccgccg tcaacggccc cgcgtcggtc gtgatctcag gcgagatgga ggcgctcgac
    15421 gagctgctgg cggcctgcga ggccgacggc acctgggcgc gcaagatccc ggtcgactat
    15481 ccctcgcact cgaaggcggt cgagcggatc gaggcgcggc tggcagagga cctcggcccg
    15541 atcgagccca agacgggcag cgtgccgttc ttctcgacgg tcgaggccga ggtggtcgac
    15601 accgcgacgc tcgacgccgg ctactggtat cgcagcctgc gcaaccgcgt gcgcttcaac
    15661 gacgccgtcg ccgccctgat cgaggacggc gcctctgcct tcctcgagat gagcccgcac
    15721 ccgggcctga ccgtcggcgt cgccgccgcc gccgacgccg ccggggcctc cgagcgcatc
    15781 gccgcggtcg gctcgctgcg ccgcggcgag ggtgggctcg agcgcttcct ggcctcgctc
    15841 gccgaggcgc acgtgcacgg cgtcgacgtg aactggtccc agctcttctc cggcaccggc
    15901 gcgcgcgtgg tcgacctgcc gacctacgcg tttcagcgcg agcgctactg gctcgagggt
    15961 gacggcggcg ccggtgacct ggccgccgca ggcctgatgg ccgtggacca cccgctgctc
    16021 agcaccggcc agctcctcgc gggtacggac gagtggctgt tcacgggccg cgcgtcgcgc
    16081 gcgacgcaga cctggatcag cgatcacgtc ctgctcgaca cggtggtcgt gccgggcacc
    16141 gcctgggtcg aggtcgccat ggccgcgggc gcggccgtcg actgcgagat gctcgacgag
    16201 ctcaccttcg agtcgccgct ggtgctcggc gagaacgagg gcgcgcagct tcaggtccgc
    16261 gtggacggag cggacccctc gggccgccgc gagttcgcga tctactcgcg cccggaggac
    16321 ccgtcgcgcg agggcgacga ccaggagtgg actcggcacg cgagcggtgc ggtcgcgtcc
    16381 gcggccgagg cggagcggtc ggagcagatc gagcggctgg cggcggaggc atggccgccg
    16441 gagggcgccg aggcgatcga cgtggacggc ctgtacgacc ggctcgtcgg gatgggcttc
    16501 gcgtacgggt cggcgttcat gggggtcgag gccgcctggc gccggggcga cgagctgttc
    16561 gccgaggtcg cgctcgacgg ggagcacgcc gccgaggcgg agcgctacgg aatgcatccc
    16621 gcgctcttcg acaccgcgat gcatcccgtg gtggccctgc tggccgagcg cgacgacggg
    16681 gcgggcagga tgctgttcca ctggaagggc atgcggcgct actccgggca ggtgacgtcg
    16741 atgcgcgttc gcctggagct ggtcagcgac gaggcgtgga acgtcgcggc gctcgacgtc
    16801 ttcggcgcgg acgtcctgtc cgtggacgcg gtggtggcgc ggccggtcga ggccgcgcag
    16861 ctcgcgctcg cgcgccgcga ccacgacgac gcgcgcctga ggctggattg ggtcgaggtt
    16921 ccggcttcgt cgaccaacgg tcacaagccg ctcttcgcgc agctcgccgg cgtggacgcc
    16981 gatggcctcg gcgagtccta cgcggacctg ccggcgctgg tcgaggcgat cgagggcggt
    17041 gcgcaggcgc ccgacgtggt cctcgcggcc atgccgcggg tcgacgccgg cgacgcggcc
    17101 gagtcggcgc gcgcgggcgc gtgccaggcg ctcgagctcg tgcagggctg gctcgccgac
    17161 gagcgcctgt ccggcgcgcg ttgcgtgctc gtgacgcgcg acggggtcgc cgtgagcgga
    17221 gacgagtcgc cggacctggc cgccgccgcg ctgtggggcc tggtgcgctc ggcgcagagc
    17281 gagcaccctg gccgcttcgg gctcctcgac atcgacggcg ccgatgcgtc ctggcaggct
    17341 gccgcggcgc tgctcgcggc gggcgagccg cagctcgcgc tgcgcgacgg cgtcgcgcgt
    17401 gtaccgcgca tcgcgcgtgc cacggcggca ccggcagcga ccgcgcccgc gttcgacccg
    17461 gacggcacgg tgctgatcac cggcggcacc ggcgggctgg gcgcgctcgt ggcgcgccac
    17521 ctcgccgccg agcacggcgt ccgccacgtg ctgctggcca gccggcgcgg caccgaggcg
    17581 ccggagtggg ctcagctcga ggccgagctg gcggagctgg acgtcgagcc ggtggtcgcg
    17641 gcgtgcgacg tcgccgaccg cgggcagctc gaggctctga tcgcctcggt cgcaccggag
    17701 cacccgctga cgtcggtgat ccacacggcc ggcgtgatcg aggacggcct ggtcgagtcg
    17761 ctgactcccg agcaggtcga ccgcgtgatg cgcccgaagg tcgacggtgc gctacatctg
    17821 cacgagctca cggagggcct cgagctggcc cagttcgtga tcttctcctc gttcgcggcc
    17881 acgctcggct cgcccggcca ggcgaactac gccgcgggca acgcgttcgc cgacgcgctc
    17941 gcgcagaggc ggcacgcgag cggcctcgcc gccaagtcgc tcgtctgggg agcgtgggcg
    18001 gaggccggcg gcatgacccg cgatcgaagt gaggcggacg cggcgcgcat ccggcgcctc
    18061 ggcgccgcgc tgctgacgga ggccgagggc ctggcgctct tcgacgcggc gctggacgcc
    18121 gacgacgagc tgccggtgct ggtgcgcctc gagtcgggaa cgctgcgcgg catggcgcgc
    18181 gacggcgtgc tgccgccgat cctgagcggg ctcgtccgca cccggccgac gcgctcgggc
    18241 gaggccgcgg actcgctcca gcggcgtctc tccggcgtgc cggaggcgga ctggcccgag
    18301 accgtcctcg acctggtgcg cgaccagatc gccgccgtgc tcggcctcga gtcgcgcgac
    18361 gaggtcgagc cgcaggtcgc cttcaaggag atgggcttcg actcgctggc cggcgtggag
    18421 ctgcgcaacc ggctgctgcg atccacgcgg ctccagctgc cggcgacgct cgtgttcgac
    18481 catccgactc ccgcctccgt ggccgagtac atgctcgggc agctccccaa gggaggaggc
    18541 ggcggtgcgg agcggccgcc gatcgacggg gagttcgacc ggatcgagcg cctggtgcag
    18601 gagatcgcga ccgacgagcg gacccgcgcg caggtcgaga cccggctgcg tgcgttcaac
    18661 gctcgcatcc agagcttcct cgtggacccc ggcgagtcgg cggacgccga cctcgacgag
    18721 gacctcgaag cggtctccga cgaagagatg ttcgccctga tcgacaagga actcggaccc
    18781 tcatgaccga cgaccaacgc ctacgtcaat acctgcgcaa ggtcaccggc gagctgcgca
    18841 cggcgcatcg ccgcgtgcgc gagctagagc agcgcgacca cgagccgatc gccatcgtcg
    18901 gcatgagctg ccgctacccg caggcggtgc aatcgcccga ggagctgtgg gaggtcgtgg
    18961 ccggcggcgt cgacgccgtg accgagtacc cggacgaccg cggctgggac ctcgagcgcc
    19021 tgtacgaccc ggaccccgac aaccccggca ccgtctactc gcgcgagggc gggttcgtgc
    19081 gcgacgtggc cgatttcgac gccgagttct tcggcatcag cccgcgcgag gcgctcgcga
    19141 tgaacccgca gcagcggatc acgctcgaga tcgcgtggga ggcgctcgag gacgcgggca
    19201 tcgacccgac ctcgctgcac gggagccaga ccggcgtgtt cgccggtgtc ttccacgacg
    19261 actacagcgt cggcccggtg cgggccatgg cggctccgga gctcgagggc tacgcgtaca
    19321 tgggcgacgc ggcgtgcatc ctgtccgggc gcgtgtccta cacgctcggc ctcgaggggc
    19381 ccgcgctgtc ggtcgacacg gcgtgctcgt cgtcgctggt cgcgatccac ctggcgagcg
    19441 aggcgctgcg caaccgcgac tgctcgctcg cgctggcggg gggcgtcacg acgatgtcca
    19501 ccccgatgtt cctgatctcg ctgagccgtc agcgggcgat gtcgccggac gggcgctgtc
    19561 gggcgttcgg tgcggaggca aacggcgtcg gcttctcgga aggcgcgggg atgctggtgc
    19621 tggagcggct ctcggacgcg cgccggctcg gccaccgggt gctggcgctc gtgcgcggga
    19681 gcgcggtgaa ccaggacggc gcgtcgaacg ggctgtccgc gccgaacggg ccctcgcagg
    19741 agcgggtgat ccgccaggcg cttgcgagcg gcggcctgac cccggccgac gtggacgccg
    19801 tcgagggcca cggcacgggc acgacgctgg gcgacccgat cgaggcgcag gcgctcctgg
    19861 ccacgtacgg gcaggagcgt gagaacggac cgctccggct cgggtcgatc aagtcgaaca
    19921 tcgggcacac gtcggccgcg gccggggtcg cgggcgtgat caagatggtg atggcgatga
    19981 ggcacggggt gctgccgcgg tcgctctact gcgaggagcc ctcgccgcac gtggactggt
    20041 cgatgggcga cgtgcggctc ctggcggagg cagagccgtg ggagccggga tcgcgcccgc
    20101 gccgcgccgg ggtctcgtcg ttcgggatca gcgggacgaa cgcgcacgtg atcgtggagg
    20161 aggcgccggc ggcgcagccc gcgcggtccg cgcccgcgga cggccctgcc gaggctgcgg
    20221 cggccgcgga gcccgtggcc gccgaggagg cgccggtcga gggggcgccc gcggcggagg
    20281 ccgcgcccgc cgagggggcg cccgcaaact cgccggccga aggcgcgccc gccgaggggg
    20341 cgcccgtgga ctcgcccgcc gaaggcgcgc ccgccgagcc cgcggctgcg cccgcacccc
    20401 tccccgccat cccgctgatc ctctccgcgc gcaccgacgc cgccctcgcc gcccaggccg
    20461 agcgcctgcg cgcgcgcctg ctcgatcgca ccgagctcga gccgctcgac gtcgcctact
    20521 cgctcgccac cggccgcgcc cgcttcgagc gccgcgccgc actcgtcgcc gccgaccgcg
    20581 acgagctgat cgcctgcctc gacgcgctcg ccgccggcga gctgcccgcc tcgggcagcg
    20641 tcggcgagcc gcgctccggc aagagcgcct tcctgttctc cggccagggc gcccagtggg
    20701 ccggcatggg ccgcgacctc tacagctcct acccggtctt cgcgcaggcc ctcgacgccg
    20761 cctgcgccga gctcgacgca cagctcgggc gctcgctccg cgagctcgtc ttcgcaaccg
    20821 agggctcgcc cgaggccgag ctgctcggtc gcaccgagaa cacccaggcc gcgctgtttg
    20881 cgctcgaggt cgccctctac cgcctgttcg agtccttcgg cctgcgcccc gactacctgg
    20941 tcgggcactc gatcggcgag ctcagcgccg cccacgtcgc cggagtgctc tcgctcgccg
    21001 acgcctgcgt gctggtcgca gcgcgcgggc gcctcatggg agcgctgccc gagggcggcg
    21061 cgatgctggc ggtcgaggcc gacgaggacg agctcgcacc cacgctcggc gagcagctct
    21121 cgatcgccgc gatcaacgga ccgcgcgcgc tcgtcgtcag cggcgagcgc tctgccgtcg
    21181 aacagtgcga ggcccactgg cgcgcgcagg gcagaaagac ctcgcggctc gacgtcagcc
    21241 acgccttcca ctcactcctg atggagccga tgctcgacga gttccgcgcc gtcgccgccg
    21301 gcctcggctt cgagccccca cagatggcgg tcgtctcgaa cctgaccggc gagctcgcca
    21361 ccgacgagct gaccgacccc ggctactggg tccgccacgt gcgcgaggcg gtccgctttg
    21421 ccgacggcgt ccgcgagctc gagcgccgcg gcgtcaccag gttcatagag ctcggccccg
    21481 acggcgtgct gacggcgatg gcgcgcggct gcgtctccga ggaccacgcc gagcgctccc
    21541 tgttcgccgc cgccatgcgc gccgagcgcg agggcccgcg cacgctcgcg gcctgcgccg
    21601 gggcgatgga ggtcgccggg ctcgggctcg actggcacgc gttcttcgcc aaccgcggag
    21661 cgcagcgcgt cgacctgccg acctacgcct tccagcgcga gcgctactgg ctggagccca
    21721 gcgagagcgc cggtgacctg accgccgcgg gccaggcctc ggccgagcac ccgctgctcg
    21781 gggccgccgt ccggctcgcc ggcgaccagg agggctggct gttcaccgga cgcctgtcgc
    21841 tcaggaccca cccctggctc gccgaccacg ccgtcgccga tgttgtgatc ctgccgggcg
    21901 ccgcgttcat ggagctcgcg ctggccgtcg ccgaccgagt cggggcgggc gagctcgagg
    21961 acctgacgct ggtcgcgccg ctggtgctca cccaggacgc ctcgctggcc gtgcagatca
    22021 cggtggccga ggccgacgag gagggacgca ggccgatcaa cgtctactcc tccgcgcagg
    22081 gggaggagcc gggcgacgag gactgggtac tgcacgcctc cgggctgctc ggggcggagg
    22141 gcgccgcggc gagcgagacg gacgccgcgc tcgcggactt cgccgccgcg agctggccgc
    22201 ccgcgggcgc cgagccggtc gacgtcgaga ccttctacga gcgcgtggcc gccgccggct
    22261 acgactacgg ccccaacttc cagggcatgc gcgcggccta ccgcaaggac ggcgagtggt
    22321 acgccgaggt cgcgctcgcc ggcgagcagg agaccgaggc cgccggctac ggcgtgcacc
    22381 cggcgctcac ggacgcggcg ctgcacacga tcgtgctgga ggcgctcgag cggctcgagc
    22441 cgggcgccgc cccggaggtc ccgttctcgt tctcgggcgt gcgcctgctc cgtcacggcg
    22501 cctcgtcgtt gcgggtgcgg atgcggacga ctcaggacga ggactccgac gcgcggaccg
    22561 tgcggctgct ggcactcgac gagtccggcg cccccgccct ggcgatcgag gcgctgaagg
    22621 cacgcgcggt cgaccaggcg agcctgaagg cgcgggcgag cgcaggtaac gactcgctgt
    22681 tcgacgtgga gtgggtcgag gttcccgccg cggacacgaa cggctcgacg ctgaacgcga
    22741 cggtgctcgg cgacgaggag ctggacctcg acagccccgc ggtcgagctc acccgccacc
    22801 cggacctcga cgcgctcgcc gaggcgatcg ccgccggagc gccggccccc gcggtcgtgc
    22861 tcgtgcgcgc cgcatcgatc gcgccggcgg acggcgggct cgcggactcg gtccacgacc
    22921 tcggtgcacg catgctcgac ctgctccagt cgtggctcgc gagcgagccg ctggccgagt
    22981 cccggctcgt gctcctcacc gacggcgcgc tggcggtctc cggcggcgag tcgcccaacc
    23041 tcgcgcaggc cgcgctgctc ggcctcatgc gcagcgccca ctcggagaac ccggggcgct
    23101 tcggggtgat cgacctcgac ggcagctcca ccgcgggcct ctacggcgcg ctcgtcagcg
    23161 gcgagccgga gctggcgctc agggagggcg tggcccgcgc gccacggctc gcccgggccg
    23221 ggtccgggac cccgctgatg ccgccggagg gcgaggccgc gtggcagctc ggcatcgaga
    23281 cgccgggcac actcgagagc ctcgtcctga tccccagctc gacggcgcgc gagccgctcg
    23341 agccgggtca ggttcgcgtc gcgatgcacg ccgcggggct caacttccgc gacgtggtgg
    23401 tcgcgctcgg cctcgtgtcc cacgcgggtg gggagctcgg cggtgagggg gccggcgtgg
    23461 tgagcgaggt cggccccggc gtcgagggcc tcgcaccggg cgaccgcgtg atggggctga
    23521 tgaccgccgc cctcgggacg acgacgatca ccgacgcccg gctgctcgcg aagatcccgg
    23581 acggctggtc gttcgccgag gcggcgtcgg tgccgatcgt cttcatgacg gcctaccacg
    23641 ggctcttcga cctcgccgac ctgaagcagg gcgaggcggt gctgctgcac ggcgcggccg
    23701 gcggcgtcgg gatggcggcg atccagctcg cggctcacgt gggcgccgag gtcttcgcca
    23761 ccgcgcaccc gagcaagtgg gagacggtcg aggggttggg cgtcgatcgc tcccacatct
    23821 cctcgtcgcg caccctggac ttcaaggacg aggtcctcga acggaccggc ggcaggggag
    23881 tcgacgtcgt cctggactcg ctcgcgggcg agttcgtcga cgcctcgctc gacctcctcc
    23941 cgcgcggcgg ccgcttcatc gagatcggca agaccgacat ccgtgacgcc gacgaggtcg
    24001 ccgcgcagca cgccggcgtc gcctaccagg cgttcgacct gctggaggcg gggcccgggc
    24061 gcatccagac gatgctgagc gaggtcgtgt cgctgttcga gcagggcgtg ctccaccacc
    24121 tgccggtctc cacgtgggac ctccgccgcg ggccggacgc gttccggcac atgcgcgagg
    24181 cgaagcacgt cgggaagatc gtgctgtgcg tgccgcagcc gccggagcgc gacggcaccg
    24241 tcctgatcac cggcggcacc ggcgggctag gcgcgctcgt ggcgcgccac ctggccggcg
    24301 agcacggcgc gcggcgactc gtgctgacga gccggcgcgg cctcgaggcc gagggtgccg
    24361 aggagctcgt ggccgcgctc gcggagctgg gctgcgaggc cgaggtggcc gcgtgcgaca
    24421 tcgccgatcg ccagcaggtc gaggcgctcc tcgccgccat tcccgccgag cacccgctca
    24481 cggcgatcgt gcatgccgct ggcgtgctcg acgacggcgt gatcgactcg ctcgacggtg
    24541 agcggctcag gaaggtcatg gcgccgaagg tcgacggcgc gctcaacctg cacgagctga
    24601 ccgaggacgc ggtgctctcg cagttcgtca tgttctcgtc ggtcgcgtcg acgctcggca
    24661 gcccggggca gggcaactac gcggcggcca acgcgttcat ggaggcgctc gcgcagcacc
    24721 gccaggcccg cgggctgccc gccaccgctc tcgcgtgggg cgcgtgggag cgggtcggcg
    24781 gcatgctcgg ggccctgagc gatgcggacc gctcgcgcgg cgagcgcatg ggcatcacgg
    24841 cgctgcccga cgagcagggc ctcgcgctgc tcgacaccgc gcgcgctgcc gcgcagccgc
    24901 tgctcgtgcc ggcgcgcctc gacagcggcg cgctccgctc gcaggcgaag gccggcgtgc
    24961 tgccagcgat cctccagggc ctcgtccgcg cgcccgtgcg ccgggccgcc gagacgaagg
    25021 gctcgctggc gacgcgcctg gcggcggcgc ccgagtccga ctggcaggcg atcgtcctcg
    25081 acctcgtcaa gagcaacgtc ggcggcgtgc tggggcacaa ctcgggcgac gccatcgatc
    25141 cgaagcgcgc gttcaaggac ctgggcttcg actcgctgag tgcggtcgag ctgcgcaacc
    25201 ggctcaccca ggcgaccggc atgcggctgc cgacgacgct cgtcttcgac catcccagcc
    25261 ccgccgcggt ggccgagcag ctcatcggaa tggcggtgcc gaaggcgaag acgaacggcg
    25321 actccgggga gtccgagctg cgcgaggtgc tcgcgtcgat cccgatccgc cgcctgcgcg
    25381 aggcggggct gctcgatccg ctcgtggagc tcgcgcgctc ggacggctcg tcctccccgt
    25441 ccgacgggga cggcgacggc ggggcgccgg cggcgtcgat cgacgagatg gacgccgagg
    25501 cgctgatccg gatgacccgc ggagaggtcg aggagagcgg gctcgagaac gcgctcgact
    25561 cctgaccgtc tgaatggccg acaaccagga acagctagtc gaggcgctgc gcacctcgct
    25621 caaggagacc gagcgactgc gccagcagaa ccggcggctg gtcgcgaagg cgacggagcc
    25681 gatcgcgatc gtgggcatga gctgccgctt cccgggcggg gcggactcgc ccgccgcgct
    25741 gtgggagctg gtcgccgagg gtcgcgacgc gatcggtgag tttccgggcg accgcggctg
    25801 ggacctcgac cacatctacg acccggatcc ggacgcgctt ggcacgagct acacccgtca
    25861 cggcggcttc ctcgccgacg cggccgagtt cgacgcggac ttcttctcga tctccccgcg
    25921 cgaggcgctg gcgatggatc ctcagcagcg gctgctcctg gagggcgcgt ggcaggcgtt
    25981 cgaggacgcc gggatggcgc cgacgagcct ccggggaagc gcgaccggtg tcttcgtggg
    26041 cgtcgtcaac tccaactacg ggatcgagct ggagtcgccg aaggaggtcg agggcttccg
    26101 cctgaccggc accacgacga gcgtggcgtc gggccggctc gcgtacacgc tcgggctcga
    26161 ggggccggcg gtctcggtgg acaccgcgtg ctcgtcgtcg ctcgtcgcgc tgcacctcgc
    26221 ctgccaggcg ctgcggcggg gcgagtgcga gctggcgctt gccggcggcg cgaccgtgct
    26281 cgccagcccg ggcctgttca tcgagttctc acgccagcgc gggctctcgc gcgacggccg
    26341 ctgcaaggcg ttcggcgcgg gtgccgacgg cacgggttgg tccgagggca ccgggctctt
    26401 cgtgctggag cggctgtcgc aggcgcgggc gaacgggcac gaggtgctgg cgctcgtgcg
    26461 cggcagcgcg gtcaaccagg acggcgcgtc gaacgggctg acggcgccga acgggccgtc
    26521 gcaggagcgg gtcatccgcc aggcgctcga gagcgcgggg ctctcgccgg acgacgtgga
    26581 cgcggtcgag ggccacggga ccggcaccac gctgggcgat ccgatcgagg cgcaggcgct
    26641 gctggagacg tatggccggg agcggtcgaa cgggccgctg tacctcggct cgatcaagtc
    26701 gaacatcggc cactcggcgg ccgcggcggg tgcgggcgcg gtggtcaaga tggtgcaggc
    26761 gctgcggcac gggctgctgc cgcggacgct gcacgccgag gagccgacgc cgcacgtgga
    26821 ctggtcggcg ggcgcggtcg agctgctcca ggagccggtc gagtggccgg cgggggagcg
    26881 cgtgcggcgt gtcgggatct cttcgttcgg cgtgtcgggg acgaacgcgc acgtgatcct
    26941 ggaggaggcg ccgcgcgtcg aggccaaggc cgctgcggct cccgaggcgg acggcgacgc
    27001 gccggccgtg gtgcgcgagg gcgccctgcc cttcctcgtc tccgccgcca ccccgcaggc
    27061 gctcggcgag caggcgggcc ggctcgccgg cttcctggag cgccgcccgg agctcgactc
    27121 ctatgccgtc gccgcggcgc tcgcgacccg ccgtgcgcag ctgccgcagc gcgccgtcgc
    27181 ggtcgccggc gaccacgcgg agctagtcgc gagcctgcgc gcgctcgagc gcggcgagct
    27241 ggccaacggc gtcgtgcgcg ggagcgcggc cggtggcggc gcgaccgcgt tcctgttctc
    27301 gggccagggc tcgcagtggg ccgggatggg ctcggtgctg cacagggagt tcccggtctt
    27361 cgccgcggcg ctcgacgagg tctgcgccga gctcgaccgc cacctcgagc gtcccttgct
    27421 cgaggtgatg ttcgccgccg agggctcgga ggaggccgag ctgctcgccg acacgcgctt
    27481 cacccaggtc gcgctgttcg cgctcgaggt cgccctcgcc cgcctcgtcg cgagcttcgg
    27541 gatcgtgccg gactacctga tcggccactc gatcggcgag ttctcggccg cccacgtggc
    27601 cggggtcttc tccttggagg atggcgcccg tctcgtggcc gagcgggcgc gcctgatggg
    27661 cgcgctgccg gccggcggcg cgatgctggc cgtcgaggcg agcgaggccg aggcgctcga
    27721 gggcctcgcc ggcttcgagg accggctcgc gctggcggcc ataaatgccc cgaccgccgc
    27781 ggtcgtcagc ggcgaggagc aggcgatcgc cgaggtcgag gcgctctggc gcgcccgcga
    27841 tcggcgcacg acccgcctgc gcgtcagcca cgcgttccac tcgcagctga tggacccgat
    27901 gctcgacgac ctgcgcgccg tggcagcgga gatcgagcta tcggcgccgc ggatcccgat
    27961 cgcctcgaac ctgaccggcg agcagctcac cgccgacgag gccacctcgc ccgactactg
    28021 ggtgcgccac ctgcgcgcga ccgtccgctt cgccgccggc gtcgagcacc tgcgcgccaa
    28081 cggcgtgacc cggttcctgg agctcggccc cgacggcacg ctcagcgcga tggcggcgca
    28141 gacggctggc gcggaggaag atgaggggga ggagcgcgag ccgctcttcg tcagcgccct
    28201 gcgcgggcgc aagggaccgc agcgcgaggc tctgctcggc ttcctggcgg ccgcccactg
    28261 cgacggcgcc gagctcgact ggagcgcgct gctcgacgac cgcggcgtcg ggcgcctcga
    28321 gcttccgacc tacgccttcc agcgctcgcg ctactggctc gtggccggcg cgagcgccgg
    28381 cgacctgacc gcggccggcc agacgtcggc cgagcacccg ctgctcggcg ccgccgtgcg
    28441 cctggccggc gagggcgagg gctggctctt caccggccgg ctgtcgctcg ccgaccagcc
    28501 ctggctcgcc gaccatgccg tcgccgacgt cgtgatcctg ccgggcgccg cgttcgtcga
    28561 gctggcgctc gcggtcgccg cgcgcaccgg cgccggcgcg ctcgaggagc tgaccctggt
    28621 cgccccgctg gtgctcgccg aggacgcggc gctggcgctc cagatcaccg tcgccgagcc
    28681 cgacgaggag ggacggcagc cgatcgccgt ctactcggca ccccaagccg ccgccgcggc
    28741 cagcgccgcc gtcggcacag agcccggcgc cgccgagccc gccgcggact ggaccctgca
    28801 cgcgcagggc cagctcggcg tgggcggagc cgacgccccc gagagcgtca ccgacgccga
    28861 gctcgccgag ctcgccgccg caccctggcc gcccgagggc gccgactcgg tcgacgtctc
    28921 gaccttctac gagcgcgtcg ccgccgccgg ctacgactac ggccccgcct tccagggcct
    28981 gcgccgcgcc taccgcgcct cctcgcccga cggcgacgag tggttcgccg aggtcgcgct
    29041 cgccagcgat caggagaccc aggccgggag ctacggcgtg cacccggcgc tctcggacgc
    29101 ggcgctccac accatcgtgc tggaggcgct cgagcggctc gagccgggcg ccgcccccga
    29161 gatcccgttc tcgttctccg gcgtgcgcct tcagcgcccg ggcgcagcat cgctgcgggt
    29221 cgcgatgcga acgagcgagc aggagggctc cgacgcgcgc acggtccgca tcctcgcgct
    29281 cgacgagtcg ggcgcgcccg ccttcgcgat cgaggcgctc gaggcgcgcg cggtcgacca
    29341 ggccgcgctc aaggcccgcg cgagcacggg ctccgacgct ctgttcgggc tcaactggac
    29401 cgaggtccag ggcgccggcg ccgacagcac ggctccatcg gccgccctcg tcggctccga
    29461 cgacagcggc gcgctcgacg gtgcctcgtt cgagctggcc cgctaccccg acctcgaagc
    29521 gctcgccgag gcgatcgccg caggggccga ggcgccgcag gtggtgctcg cgcgcgcggt
    29581 cgcgctcgcg gcgcccgtca tcgacgcgcc caccagcgac gggtcctccg cgagcctcgc
    29641 cggctcggtc cacgacctca ccgcgcgcac gctcgcgctg ctccagtcct ggctcgccgc
    29701 cgagccgctc gccgagtccc gcctcgtgct cctgaccga
//
LOCUS       DASVPL010000127         5440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_132102, whole genome shotgun sequence.
ACCESSION   DASVPL010000127 DASVPL010000000
VERSION     DASVPL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132102"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..528)
                     /locus_tag="VF517_06500"
     CDS             complement(79..528)
                     /locus_tag="VF517_06500"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8052624.1"
                     /translation="MGLIEGEKTVEIDAPIERCFEIAADIEGAPEWQGSLQDVDVLER
                     DSERRAELVETKSDAKVKSVTAVLRFSYDPPDGVRWVQEKGDTKSLEGWWKFEDLGEG
                     RTRATYGLAADPGRMLGMLLRGPAEAKVRDFLLGGAAEGLKKKAESG"
     gene            complement(540..1049)
                     /locus_tag="VF517_06505"
     CDS             complement(540..1049)
                     /locus_tag="VF517_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052625.1"
                     /translation="MRTPVALAALLAALSLAGCGGDDESGSGGDRPAQRTGGDEPAQK
                     AKGGAACIESWNANASPDVKGYTVAGVSEEDTMAGTYRGPEEFEAQTEAETVTVQPGD
                     CVVAQVTSTETEYVFVESAPSPTRGKSWHNLEEEGTPLAKPGAAQLKDVVTAKLKGYG
                     PEGKLEPNE"
     gene            complement(1081..1830)
                     /locus_tag="VF517_06510"
     CDS             complement(1081..1830)
                     /locus_tag="VF517_06510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8052626.1"
                     /translation="MSRLIATALAVAALLAVAGVALATAGGGAREQQRRSAAPDARAN
                     QVVDLSSAAGSETARRDERTRPREDVGDQDLAAIERDIKRRAERVLGASPDDGIAPGA
                     PSDAEVRSDLRAMRSERDGGGPIKRGSGDFIYPIDGAFSSPFGMRWGRLHAGVDLAAP
                     AGRPIRAAAAGRVILAAPTGGYGNYTCIAHNSSISTCYAHQSRFGVRVGESVRQGEVI
                     GYVGNTGHSFGAHLHFEVRIGGRPVDPMKYL"
     gene            complement(1887..3299)
                     /locus_tag="VF517_06515"
     CDS             complement(1887..3299)
                     /locus_tag="VF517_06515"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HEX8052627.1"
                     /translation="MGTSDGTVIGTTQILNNGPASSRFNLVLLSEGYQAGADMTQWAT
                     NAQQFVDYLRGTPPFDDPTVMGRINVFRVDVTSTDRGADKPAACFGGAALTAATYFDA
                     TYCAGGLDRLLVANNAIVTGVLAAQVPAWHQAVVVVNDTEWGGSGGTIGVTSLSTGWQ
                     GIAVHEMGHSAFALADEYEYYQGCGSGETGHDTYTGPEPGQPNITIDANRATNEWRAL
                     IDAATPMPTTTNANCAVCDPQPNPVGARTVGAFAGAGYYHCGIYRPEYDCMMRNLTRY
                     FCGACARAIRGTLRPFTTALARVDVSAPAVNFVFDPDGTITVSDIGPPLTPNGASGAG
                     FLQSRLYPQAPAGTPAAGLYPYEYRVSLDGASGGGPGACVRSLSLDFGPVQSLDYNGS
                     GTVEQVFVTTQGGLGSVRPSAVELAGDRLTFTFDPPVCPGATSYFFGLASAHPPHDVT
                     AEVRDVAGNSYSLPAKAPAF"
     gene            complement(3299..3772)
                     /locus_tag="VF517_06520"
     CDS             complement(3299..3772)
                     /locus_tag="VF517_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052628.1"
                     /translation="MAEGQGSLRLTFSYEGRRVELQSVEPVDAVAPAPPVSPRGTAGF
                     ALEVHGRGGTVLFKRMLDEPFGESVEVPAGDRDPERPFERAAVENPSGTFIVMVPALA
                     AGAEVALFDRRGDKRRGAAHDEPVARFELPARGRPGGPGKQGRGRHGPEGEKGRA"
     gene            complement(3818..4540)
                     /gene="ubiE"
                     /locus_tag="VF517_06525"
     CDS             complement(3818..4540)
                     /gene="ubiE"
                     /locus_tag="VF517_06525"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX8052629.1"
                     /translation="MTSTGQETGRAPGTLEHTQVRAMFDRIAGIYDLLNQVMTAGLHH
                     DWRRRAVDLAGVGPGDRVLDVAAGTGDLTIELARRVEPGGEAVGCDFSEQMLVRAREK
                     APGLRFETGDALALQYADGEFDAATVGFGARNFSDLDRGLREMARVVRPGGRVVVLDF
                     TTPTRPPLSTFFRLWFDRIVPVIGRLVGQPEAYDYLPNSVKRFPVPRELAATMSRCGM
                     TNIRYILTAGGIVTLHVGEVER"
     gene            complement(4543..4851)
                     /locus_tag="VF517_06530"
     CDS             complement(4543..4851)
                     /locus_tag="VF517_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEX8052630.1"
                     /translation="MLALVTTHARGAAAAALACAALGVFAGCGEATLDSKSAEDQITE
                     KSKAQGLEVKSVDCPDDIKAKKGEKGTCELTTAAGDKADVKLTMLDDDGKFEFEVQGQ
                     "
     gene            4914..>5440
                     /locus_tag="VF517_06535"
     CDS             4914..>5440
                     /locus_tag="VF517_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8052631.1"
                     /translation="MEAPVRLRQAVLAAAELDGVAGRLREELGLGEPYADPGVGAFGL
                     RNAVFALGDAFLEVVAPTQPGTAAGRYLERHGGDGGYMLIFQVDDLSGARARAEELGV
                     RTVWGIDLPDIAATHLHPADMRGAIVSIDRPVPPESWRWGGPGWIGEGGTGAPGRLEG
                     ATVAVTDPEAVAERWA"
BASE COUNT          676 a         1988 c         1976 g          800 t
ORIGIN      
        1 cccgcgcgta gcggaggaag cggtcgagga cgtcgtcggc gagcgacttc gccagcggcg
       61 aggtgaaggg tgccggcgtc agcccgactc ggccttcttc ttgagtccct cggccgcgcc
      121 gccgagcagg aagtcgcgca ccttcgcctc ggcggggccg cgcagcagca tgccgagcat
      181 gcggccgggg tcggcggcga gaccgtaggt cgcgcgcgtg cgcccctccc ccaggtcctc
      241 gaacttccac cagccctcga gcgacttcgt gtcgcccttc tcctgcaccc agcgcacccc
      301 gtccggcggg tcgtagctga accgcaggac cgcggtcacg ctcttcacct tcgcgtcgga
      361 cttcgtctcg accagctccg cccggcgctc ggagtcgcgc tcgagcacgt cgacgtcctg
      421 gagcgagccc tgccactccg gcgccccctc gatgtcggcc gcgatctcga agcagcgctc
      481 gatcggcgca tcgatctcga ccgtcttctc accttcgatc aggcccatgt ggcgcgagac
      541 tactcgtttg gctcgagctt gccctccggg ccgtagccct tcagcttcgc ggtcacgacg
      601 tccttcagct gcgcggcgcc cggcttcgcg agcggcgtcc cctcctcctc gaggttgtgc
      661 caggacttgc ctcgggtcgg gctcggcgcc gactccacga acacgtactc ggtctccgtg
      721 gaggtgacct gcgcgaccac gcagtcgccg ggctgcacgg tgacggtctc ggcctcggtc
      781 tgggcctcga actcctccgg ccccctgtag gtgcccgcca tcgtgtcctc ctccgagacc
      841 ccggcgaccg tgtagccctt cacgtcgggc gaggcattcg cgttccagga ctcgatgcag
      901 gcggcgccgc ccttcgcctt ctgggcgggc tcgtcgccgc cggtccgctg ggcgggcctg
      961 tcgccgccgc tgccgctctc gtcgtcgccg ccgcagccgg ccaggctgag ggccgcgagc
     1021 agcgcggcga gggcgaccgg ggttcgcatg ggcgcgaacc tagcgcgtgg cgcggccgcg
     1081 ctacaggtac ttcatcgggt cgaccgggcg gccgccgatg cgcacctcga agtgcaggtg
     1141 cgcgccgaaa gagtggccgg tgttccccac gtagccgatg acctcgccct ggcgcacgga
     1201 ctcgcctacg cgcacgccga agcgcgactg gtgggcgtag caggtggaga tcgacgagtt
     1261 gtgggcgatg caggtgtagt tgccgtagcc gccggtcggc gcggcgagga tcacgcggcc
     1321 ggccgcggcc gcgcggatcg ggcgtccggc cggcgcagcc aggtcgacgc ccgcgtggag
     1381 ccggccccag cgcatgccga agggcgagct gaaggcgccg tcgatcggat agatgaagtc
     1441 accggagccg cgcttgatcg gcccgccgcc gtcgcgctcg ctgcgcatcg cacgtaggtc
     1501 gctgcgcacc tcggcgtcgg acggcgcgcc aggcgcgatg ccgtcgtcgg gcgaggcgcc
     1561 caggacgcgc tctgcacgcc tcttgatgtc gcgctcgatc gcggccagat cctggtcgcc
     1621 cacgtcctcc cgcgggcgcg tgcgctcgtc gcgccgggcg gtctccgagc cggctgcgga
     1681 actcaggtcc acgacctggt tggcgcgggc gtccggcgcc gcgctgcggc gctgctgctc
     1741 gcgtgcgcct ccgccggcgg tcgcgagcgc cacgccggcg acggcgagca gcgccgccac
     1801 ggccagcgcg gttgcgatga ggcggctcat cccgacctct atcggcgcga gcgcacatgg
     1861 cccttgaggg ggaaacgggg ctgcgtctag aaggccggcg ccttggccgg cagggagtac
     1921 gagttcccgg ccacgtcacg gacctcggcg gtcacgtcgt gcggcggatg cgccgaggcg
     1981 aggccgaaga agtagctcgt cgcccctggg cagaccgggg ggtcgaaggt gaacgtcagg
     2041 cggtcgcccg cgagctcgac cgccgagggc cgcacgctgc cgagccctcc ctgggtggtg
     2101 acgaacacct gctcgaccgt gccactgccg ttgtagtcga ggctctggac cgggccgaag
     2161 tcgaggctca gcgagcgcac gcaggctccg ggcccgccgc cggaggcgcc gtcgagggac
     2221 acccggtact cgtaggggta gagccccgcc gcgggcgtcc ccgcgggcgc ctgcgggtac
     2281 aggcgcgact ggaggaagcc cgcgccgctc gccccgttcg gcgtgagcgg cggtccgatg
     2341 tcgctcaccg tgatggtgcc gtccgggtcg aagacgaagt tcaccgccgg ggcgctcacg
     2401 tccactcgcg ccagggcggt cgtgaacggg cgcagggtgc cgcggatcgc ccgcgcgcac
     2461 gcgccgcaga agtagcgcgt gaggttgcgc atcatgcagt cgtactcggg gcggtagatg
     2521 ccgcagtggt agtagccggc gcccgcgaag gcccccaccg ttcgcgcacc caccgggttc
     2581 ggctgggggt cgcacaccgc gcagttcgcg ttcgtcgtgg tcggcatcgg cgtggcggcg
     2641 tcgatcaggg cgcgccactc gttcgtggcg cggttcgcgt cgatcgtgat gttcggctgg
     2701 ccgggctccg ggccggtgta ggtgtcgtgc ccggtctcgc cggagccgca gccctggtag
     2761 tactcgtact cgtcggcgag agcgaaggcg gagtggccca tctcgtgcac cgcgatcccc
     2821 tgccagccgg tggacagcga ggtgacgccg atcgtcccgc ccgacccgcc ccattcggtg
     2881 tcgttcacga cgacgaccgc ctggtgccac gcgggcacct gtgcggcgag cacgcccgtg
     2941 acgatcgcgt tgttggcaac gagcaggcgg tcgaggcccc cggcgcagta cgtcgcgtcg
     3001 aagtacgtcg ccgccgtcaa agccgccccg ccgaagcagg ccgcgggctt gtcggcgccc
     3061 cggtcggtcg aggtcacgtc gacccggaac acgttgatgc ggcccatcac agtcgggtcg
     3121 tcgaacggcg gcgtgccgcg caggtagtcg acgaactgct gggcgttcgt ggcccactgc
     3181 gtcatgtcgg cgccggcctg gtagccctcg gacagcagca cgaggttgaa gcgcgagctg
     3241 gcgggaccgt tgttgaggat ctgcgtcgtc ccgatgacgg tgccgtcgct cgtgcccatc
     3301 aggcgcggcc cttctcgccc tcgggtccgt gccgcccgcg cccctgcttt ccggggcccc
     3361 ccggccgtcc ccgcgcaggc agctcgaagc gggcgaccgg ctcgtcgtgc gccgcccccc
     3421 ggcgcttgtc accgcggcgg tcgaacagcg cgacctccgc gccggccgcg agcgcgggca
     3481 ccatgacgat gaacgtgcca ctcgggttct cgaccgccgc gcgctcgaac gggcgctcgg
     3541 ggtcgcggtc gcccgccggc acctccacgg actccccgaa cggctcgtcc agcatgcgct
     3601 tgaagagcac cgtaccgccg cggccgtgca cctcgagcgc aaagcccgcc gtgccgcgcg
     3661 gggacaccgg tggcgcgggc gccactgcgt cgaccggctc cacgctctga agctcgaccc
     3721 gccggccttc gtaggagaag gtcagccgca gcgatccctg tccttcggcc atcctcgtcg
     3781 gagactacgc ccgttcaggc atgcgcgccg aaactattca gcgctcgact tcgcccacgt
     3841 gcagggtcac gatccccccg gccgtgagga tgtagcggat gttcgtcatc ccgcagcgcg
     3901 acatcgtcgc ggcgagctcc cgcggcaccg ggaagcgctt caccgagttc ggcaggtagt
     3961 cgtacgcctc gggctgtccg accaggcgtc cgatcaccgg cacgatccgg tcgaaccaga
     4021 ggcggaagaa cgtggacagc ggcggccgcg ttggcgtggt gaagtcgagc accacgaccc
     4081 gcccgccggg ccggaccacg cgcgccatct cgcgcagccc ccggtcgagg tccgagaagt
     4141 tgcgcgcgcc gaacccgacc gtggccgcgt cgaactcgcc gtcggcgtac tggagcgcca
     4201 gcgcgtcgcc ggtctcgaac cgcagccccg gcgccttctc gcgcgcgcgc acgagcatct
     4261 gctcggagaa gtcgcagccg acggcctcgc cgcccggctc gacgcgccgc gcgagctcga
     4321 tcgtcaggtc gccggtgccc gcggccacgt cgagcacccg gtcgcccggc cccacccccg
     4381 ccaggtccac ggctcggcgg cgccagtcgt ggtgcaggcc cgcggtcatg acctggttca
     4441 gcaggtcgta gatcccggcg atccggtcga acatcgcccg gacctgcgtg tgctcgagag
     4501 tgccgggcgc gcggcccgtc tcctgaccgg tggacgtcac ggttactggc cctgcacctc
     4561 gaactcgaac ttgccgtcgt cgtcgagcat ggtgagcttc acgtcggcct tgtcgccggc
     4621 ggcggtcgtg agctcgcagg tgcccttctc gcccttcttg gccttgatgt cgtccgggca
     4681 gtcgacgctc ttgacctcga ggccctgtgc cttgctcttc tccgtgatct ggtcctcggc
     4741 gctcttcgag tcgagcgtcg cctcaccgca gccggcgaac acgccgagtg cggcgcaggc
     4801 gagagccgcg gccgcggcgc cgcgcgcgtg ggtggttacg agtgcaagca tcagggtttc
     4861 tccgtttcgg aatcgggatg atgagttggt caggcggcgc tagcgtacgc accatggagg
     4921 cgcccgtgcg actgcgccag gcggtgctgg cggccgccga gctcgacggc gtggccgggc
     4981 gactgcgcga agagctcggc ctgggcgagc cgtacgcgga cccgggcgtc ggcgcgttcg
     5041 ggctgcgcaa cgcggtgttc gcgctcggcg acgcgttcct ggaggtggtc gcgccgacgc
     5101 agcccggcac cgctgccggg cgctacctgg agcgccacgg gggcgacggc ggctacatgc
     5161 tgatcttcca agtcgacgac ctctctggcg cgcgggcgcg cgcggaggaa ctcggggtgc
     5221 gcaccgtgtg ggggatcgac ctgccggaca tcgcggccac gcacctccac cccgccgaca
     5281 tgcgcggcgc gatcgtgtcg atcgaccggc cggtgccgcc ggagtcgtgg cgctggggcg
     5341 gccccggctg gatcggcgag ggcggtaccg gcgcgccggg acggctcgag ggcgcgacgg
     5401 tcgcagtgac cgatcccgag gcggtggccg agcgctgggc
//
LOCUS       DASVPL010000128        14979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_290124, whole genome shotgun sequence.
ACCESSION   DASVPL010000128 DASVPL010000000
VERSION     DASVPL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14979
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290124"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /gene="mptB"
                     /locus_tag="VF517_06540"
     CDS             <1..1306
                     /gene="mptB"
                     /locus_tag="VF517_06540"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HEX8052632.1"
                     /translation="GSAARPAPGVLALGALCALYIALAVLPAAAGSNLVLAVEHGGGS
                     PGWLLGPLRFGGLDDADGPLAGPLFYAGLWLALALYAAVLLRARDVGPRAALGAIAGL
                     HLLFLLAPPLLSQDVFSYIAYARLGVEHSLDPYTHAPVDIPNDAVYPFAGSKGAESVY
                     GPAFTLLTYPLAPLGVAGAYWVLKVIAALSSLGIVAFVWRAARRLGRDPLPAAMFVGL
                     NPLLLVHDVAGAHNETLVVMLTTAGLAAWAAGSPRAASAWGTAAAGLKASAGLVVPFL
                     IAAARPRWPGALAAAVIAGLAIVVLALAGFGTNALDALGVLSSNQDRTSSWSFPYKTA
                     ELLGALLPGDRLDYRGLTRALYGLAFAAAFVWLMLRTWRGASPIAMAGWATLAILVAS
                     AWLVPWYVLWLLPLAALAGDRRLTWATVALCAWMLPIAIPLD"
     gene            1311..2348
                     /gene="thiL"
                     /locus_tag="VF517_06545"
     CDS             1311..2348
                     /gene="thiL"
                     /locus_tag="VF517_06545"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX8052633.1"
                     /translation="MAERDLIRAFRELLAVRGERVLRASGDDAAVVRAAGVAVTSIDS
                     VVDGVHFERATHSMADIGHKALARGLSDLAAMGAAAGEAYVALTAPADLGERDAIELV
                     GAMEELAAATGTTIAGGDVTSGPTLALAVSVTGWAESEHDLAYRDGARAGDLVGVTGE
                     LGGAAAGLLLLRGGGQAAGAAPGAAAPPAGPTPLAEHAGLAPAERDALLLRHRRPEPR
                     LAAGRALAAAGVTAMIDLSDGVATDAGHIAAESGVAVELRLEALPVAAGVEPVARAAG
                     RDPLELAAAGGDDYELLFSAAPGERDTIERAARTAGTCVRWLGRVETGSGVRLVGPGG
                     EPVALAGFEHL"
     gene            complement(2492..4282)
                     /locus_tag="VF517_06550"
     CDS             complement(2492..4282)
                     /locus_tag="VF517_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052634.1"
                     /translation="MIEPRIYRAAFLPALLAAIVTMFALESQPRPLPQAAPADVLFEG
                     ASAAETVRAIVERTPDRRAGTPGDAAVAQRVAGAFRRFGLTTTVDRFEDEGVRLVNVL
                     GRRPGLSRRQVVVLVSRDALSVPDATGSAADTAALIEFGRVLEGRATKKTIVLASVDG
                     AGRGHAGARRLARTVAGPGPVDAVIVLSNLGARRPRGPMLIGSSNSDERASIGLRRTA
                     ANALRNEVGEDGGGFGSPLTQLGRLAFPVGLGPQAVLMEEGLTAIRMSASGELAPPRD
                     QRDLADLATERYEEFGRAALRFTFTLDEQARLPERGPRSYITLAGRVAPAWSISLLAL
                     TLILPVLFAGVDALARVRRRGEDVLPWLPWVAAGIAPFAVGLALAELLVLVGLADDAP
                     PAPLEPSAAEAHGSAIGVLAAVALTIGLAWIVTRTSLGRGERPRPDPAAPGAAVVVTL
                     ALCALALTAWAINPYTGLVLIPPLHAWMLAVLSGVPARTRAWMALVGLLPAALIVFTY
                     AQHLRLGPLDGAWYLFLLVTGHHVGLASALLGCVALGLLASVIGIVVAHARRSEPRAP
                     KVPRSGRRRSGPDERPISGPAGPPLERIRR"
     gene            4341..5042
                     /locus_tag="VF517_06555"
     CDS             4341..5042
                     /locus_tag="VF517_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8052635.1"
                     /translation="MSTEAPRILLVDDEQSVQTLLSYPLRKEGYDVVPASTGREALER
                     FRDAHFDLVVLDVMLPEVDGFDVCKELRAKSSVPIIMLTAKTEEVDKVLGLELGADDY
                     ITKPFSMREFSSRVKAVLRRAGLARNGDAVEEPVEHGELRIDFAKRSVELRGEPVRLT
                     YVEFEILSALVSSPGRVFSRGMLLERLWGDSAYRDPRTIDVHIRHLREKLERDAREPE
                     LIFTVRGVGYHFRDR"
     gene            5080..6501
                     /locus_tag="VF517_06560"
     CDS             5080..6501
                     /locus_tag="VF517_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8052636.1"
                     /translation="MRLRSLQNKLALLFLAITALAFAAAIFVVLPRLETQLQDERVRD
                     VERVAESGTPRIQRVVGQNIAAGQLDELVRAVADAGSVRVTLLGTQRSSTGGTDFYPL
                     SDSNAERRVSPDYDLAAEAYRAKRPGETFRRRADSEGRVAQAIFYRGQPVWIAIYSRP
                     FEDVSDSVALVRNRLILAAVVALLVALIGGFLVARALARRVRRLEHAAQEVTAGRPVP
                     PLPVDSNDELGELTRAFNEMQRQLAVVDRARKDFIANASHELRTPIFSLAGFVELLED
                     DQLDEATRRHFLETMREQVERLQKLAVDLLDLSRIDAGSLQLAPEDVDLRELARDVAL
                     EFAPALDRHRTELEMLLPEGPVEAVCDRGRTVQILRILLDNALRHTPEGTDVTVTAGR
                     HNGAAELAVADTGPGIDPAARQQLFERFYTADAARGSGLGLAIACELAERMEGRIELD
                     AAPGRTVFKLALPASSEPEPEVS"
     gene            6498..7676
                     /locus_tag="VF517_06565"
     CDS             6498..7676
                     /locus_tag="VF517_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8052637.1"
                     /translation="MRGIARVGTAALAAASLALGAGACTDDGDGGSRGDYQEASPPTE
                     RTQVRVPEGLDRGPFDARRIYRALAPGVVTVLAQIDGGVVGDQQGIGSGFVLDGDGYV
                     ATNAHVIRTDPPDLERADAVYVELADGNRVEAKVVGDDLFSDVALLKVDPRGLTLTPL
                     ELGRSSDLTVGEPVAAIGSPFGEPQSLSVGVISALDRSIQSLTSFKTEDAIQTDAAIN
                     HGNSGGPLIGRDGRVIGINAQIQSTGGGGEGVGFAVSVDVVRRSLAQLREDGSAEYAY
                     AGITSQDLYPQLARRLGYDVGRGALVRGVEGGGPADDAGIEAGDERIEFQGQRDIPRG
                     GDVIVAIDGHPVRGSSDIAEAIGRLRPGDRVVFDVARGGERRKVEVELGARDATPAGG
                     "
     gene            7681..8625
                     /locus_tag="VF517_06570"
     CDS             7681..8625
                     /locus_tag="VF517_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8052638.1"
                     /translation="MASAELVRELALALRERVLPLLGSHAGRAHAGDGAGGDVTFAID
                     EEAESYLESFLAERAPDTAFYSEDRGLVVPAGGRATEVLVVDPIDGTRPAMAGLESCC
                     VSVAAAPLRDGVRMGDVTAGCVVEIPSGTIFLAERGRGLIEGPPVVLSPNDRLDRMFW
                     TYGFRGRPARALTEVLAELIDSSSVGGATFDLGSAAFDMTRVITGQLDAYVEPGPLLV
                     DEVPGMRAEFERVGGGAVLNNSPYDLAGAALCVTEAGAVLTDARGRSLDDRPLLGSGA
                     DFQMSVVTSSNPALHELILAAVDAGVARVGSGRVDPAL"
     gene            8631..9035
                     /locus_tag="VF517_06575"
     CDS             8631..9035
                     /locus_tag="VF517_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052639.1"
                     /translation="MEAAAPNRTVTLAVPAKPDYVVLARLALSAVCRIAPLELEAAAD
                     LKLAITEAATFLMGGDRRSEAREDEESEQMVFAFDLRDDEIEVRVRGTEQRTFSEEER
                     ELSRAIIDATVDDCRYEDGTMVLKKRLGLPGE"
     gene            9067..10305
                     /locus_tag="VF517_06580"
     CDS             9067..10305
                     /locus_tag="VF517_06580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8052640.1"
                     /translation="MLQPVGVGHKALADYTHLVGRPLIEEIRTLSDQMAGLRVLHLSA
                     TAFGGGVSEILYTLIPLMRDVGIDAEWHVIIGREEFYNATKLMHNALQGHPLDLDPHE
                     WKVYEEYNEINARELTGDWDVIIVHDPQPLAVRQHCPEKSKNWIWRCHIDLSTPHPPT
                     VARLVPLIAEYDLGSVWHLQQYVPAGLNGAGPEHIIPPAIDPLSPKNMALSPEDAAFV
                     CDQFGVDVDRPLMCQVSRFDPWKDPHGVIDAYRIVKEEVPEVQLALVGSMATDDPEGW
                     EFFNSTLEYAGGDPDIKILNNLNQVGSIEVNAFQSQAEVVIQKSTREGFGLTVSEALW
                     KARPFIGGDVGGIPLQVQDGTTGYLVSSPEECADRSLRILREPELGKQLGRAGKEHVR
                     QHFLTPRLLRDWLRIFSDLK"
     gene            10302..11798
                     /locus_tag="VF517_06585"
     CDS             10302..11798
                     /locus_tag="VF517_06585"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX8052641.1"
                     /translation="MTSDTPLVLVSNRGPATFEPDDAGELQPSRGGGGLVTALKGLVD
                     HRDALWIASTLTEGDSEMSRRSGGRSFECDVDETTYRLRLVDSDPDAYDRFYNVIANP
                     MLWFIQHYLWDLSNAPDISRDEVVAYEEGYRVVNEDLARAVVEEVGDLGDAVVMLHDY
                     HLYTAPKVIRAERPDLFLHHFIHIPWTQPDAWRVLPSDMREDIYEGLLANDIVGFHTQ
                     AYRRNFLLCCRELFGLDVDMEAGVVRHDERETWVRHYPLPISASSFRKTAQRPAVHGY
                     EREVLRRRREHLILRVDRADLSKNVLRGFTAFDLFLDQHPEFREEVTFIAQMMPSRQD
                     VPEYAEYLEKIEALVAVVNHRHGTTDWMPIDLHLVDNVEQAIARYKHYDLLMVNAMFD
                     GMNLVAKEGPLVNERHGVSLLSENTGAHEELGEFALSVNPFDVQEQADAIHRALTMSA
                     EERSWRAEGLKKIISSRDPGDWVDDQLEDIDAKRSGARLGPGGVVAAA"
     gene            complement(12042..12569)
                     /locus_tag="VF517_06590"
     CDS             complement(12042..12569)
                     /locus_tag="VF517_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052642.1"
                     /translation="MRACPPEHAPPAAAPGTRLGGPSAAPHHTARTARGSSTGNHATS
                     LDASPTRAGVPVTPPFAARVIVVVAGALALAVLVSEVERGTALAAALTKAGLVAGLVM
                     VLGGLGGRLWAGAIVTSAQLPGGTGLAFAENSASIADLEEDLSHLEEETRSRLLRLER
                     DLLGPSGNGDGGTMQ"
     gene            12606..12812
                     /locus_tag="VF517_06595"
     CDS             12606..12812
                     /locus_tag="VF517_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052643.1"
                     /translation="MALRFARGRGVSVPVELRRGSGSRSRWPASGAGVLPDAAGGTAE
                     AGGSPGTRGGTPAGGSTGSRGSSD"
     gene            12997..13413
                     /gene="rpsF"
                     /locus_tag="VF517_06600"
     CDS             12997..13413
                     /gene="rpsF"
                     /locus_tag="VF517_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX8052644.1"
                     /translation="MPNLYDLMVMLDPDAPEERRTEILSNIETMVANGGGTVVGHHDW
                     GVRRIAFEIDHRPEAAYHLYQFESGSNETLERLNHTLRIADGVLRFRIIKLKPGSPPP
                     PAPRQEAPRPPRERPQETRVAARAAADADQPDREDA"
     gene            13558..14028
                     /gene="ssb"
                     /locus_tag="VF517_06605"
     CDS             13558..14028
                     /gene="ssb"
                     /locus_tag="VF517_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX8052645.1"
                     /translation="MAATNINRVVMTGNLTRDPELRSTNSGTPVCGLRIACNTRRKDG
                     SGNWVDKPNYFDVTVWGAQGENCSNYLSKGRPVAIDGRLEWREFTDKDGNNRQAVEIV
                     ADSVQFLGSREGGENGGQNGGRFTPQSDVPTNTDDFAPAPVGGGSSADSDDIPF"
     gene            14192..14464
                     /gene="rpsR"
                     /locus_tag="VF517_06610"
     CDS             14192..14464
                     /gene="rpsR"
                     /locus_tag="VF517_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX8052646.1"
                     /translation="MAKARGRQQTRRRDRGKPATGGRRKPCQFCKDKVDVVDYKDVAT
                     LRRAMSEKGKIRSARITGSCRRHQRQLARAVKRARELGLLPYVSDR"
     gene            14481..14933
                     /gene="rplI"
                     /locus_tag="VF517_06615"
     CDS             14481..14933
                     /gene="rplI"
                     /locus_tag="VF517_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX8052647.1"
                     /translation="MAQAILLEDVDTLGERGEIVDVSPGYLRNYLLPRKLAHAATPGA
                     LAEAKRRMEAAERAAREAEERAQENAALLSRTVLTISHPAGEDGRLFGQVTAQEIADA
                     IRQARSLKLDRRKIQLEEPIKTTGTHMVEVEVHDGVMARVKTIVTAAG"
BASE COUNT         2079 a         5484 c         5412 g         2004 t
ORIGIN      
        1 cggctccgcc gcgcgccccg caccgggcgt cctcgccctc ggcgcgctct gcgcgctcta
       61 catcgccctc gcggtgctcc ccgcggcggc cggctcgaac ctcgtgctcg ccgtcgagca
      121 cggcggcggc tcgcccggct ggctgctcgg gccgctgcgc ttcggcggcc tcgacgacgc
      181 cgacggtccc ctcgccggcc cgctgttcta cgcgggactc tggctggcgc tcgccctcta
      241 cgcggcggtg ctgctgcgcg cccgcgacgt cggcccgcgc gccgcgctcg gagccatcgc
      301 gggcctgcac ctgctgttcc tgctcgcgcc gccgctgctg tcgcaggacg tcttctccta
      361 catcgcctac gcccggctgg gcgtggagca ctccctcgac ccgtacacgc acgcgccggt
      421 cgacatcccg aacgacgccg tctacccgtt cgccggatcg aagggcgcgg agtcggtcta
      481 cggccccgcg ttcacgctgc tgacctaccc gctcgcgcca ctcggggttg ccggcgcgta
      541 ctgggtgctg aaggtgatcg cggcgctgtc gtcgctcggc atcgtggcgt tcgtctggcg
      601 cgcggcgcgg cggctcggtc gcgacccgct ccccgccgcg atgttcgtcg ggctgaaccc
      661 gcttctgctc gtgcacgacg tggccggcgc acacaacgag acgctcgtgg tgatgctgac
      721 cacggccggc ctcgccgcgt gggcggctgg ctcgccgcgg gcggcgtcgg cctggggcac
      781 cgcggccgcg gggctgaagg catccgcggg gctcgtcgtg ccgttcctga tcgccgcggc
      841 ccggccgcgc tggccgggcg ccctcgccgc cgcggtgatc gccgggctgg cgatcgtcgt
      901 gctcgcgctc gccggcttcg gcaccaacgc cctcgacgcg ctcggcgtgc tcagctcgaa
      961 ccaggaccgg acctcgagct ggagcttccc gtacaagacc gccgagctgc tgggggcgct
     1021 gctaccgggc gaccggctcg actaccgcgg gctgacccgg gccctctacg gcctcgcgtt
     1081 cgccgccgcg ttcgtgtggc tgatgctgcg cacctggcgc ggcgcgagcc cgatcgccat
     1141 ggcgggctgg gccacgctcg cgatcctcgt cgcctccgcc tggctcgtgc cctggtacgt
     1201 cctctggctc ctgccgctgg ccgcgctggc cggcgaccgc cggctcacgt gggcgaccgt
     1261 ggcgctgtgc gcatggatgc tcccgatcgc gatcccgctc gactgagccg gtggccgagc
     1321 gcgacctgat ccgggcgttc cgcgagctgc tcgcggtgcg cggcgagcgc gtgctgcgcg
     1381 ccagtggcga cgacgccgcg gtggtgcgag cggcgggcgt ggcggtcacg tcgatcgact
     1441 ccgtggtgga cggcgtgcac ttcgagcgcg ccacccactc gatggccgac atcggccaca
     1501 aggcgctcgc gcgcgggctg tcggacctcg ccgcgatggg agcggcggcc ggggaggcct
     1561 acgtggcgct cacggcgccg gcggacctgg gggagcggga cgcgatcgag ctggtgggtg
     1621 cgatggagga gctagcggcg gccaccggca cgacgatcgc cggcggcgac gtgacctccg
     1681 gccccacgct ggcgctggcc gtgagcgtga ccggctgggc ggagtccgag cacgacctcg
     1741 cgtaccgcga tggcgcgcgg gcgggcgacc tggtcggcgt gaccggcgag ctgggcggag
     1801 ccgccgcggg cctgctcctg ctgcgcggcg gcgggcaggc cgccggagcc gcgccgggcg
     1861 cagccgcgcc tcccgccggg cccacgccgc tcgcggagca cgccggcctc gcccccgccg
     1921 agcgcgacgc gctcctcctg cgccaccgcc ggcccgagcc ccggctcgcc gccggccgcg
     1981 cgctcgcggc cgcgggcgtc acggcgatga tcgacctgag cgacggggtg gccaccgacg
     2041 ccggccacat cgcggcggag agcggcgtgg ccgtcgagct gcggctcgag gcgctgccgg
     2101 tcgcggccgg ggtcgagccc gtcgcgcggg cggcggggcg cgatccgctc gagctggcgg
     2161 ccgcgggcgg cgacgactac gagctgctgt tcagcgcggc gcccggcgag cgcgacacga
     2221 tcgagcgggc cgcgcggacc gccggcacct gcgtgcgctg gctaggccgg gtcgagacgg
     2281 ggtcgggcgt gcggctcgtc ggccccggcg gagagccggt cgccctcgcg gggttcgagc
     2341 acctctgagc ccatcaggag gtcgcggagc gggccgcggc tgagcatggc ccccgcaacc
     2401 gcgtgcggat cgaccacgta ctcgcccgcc ctgatctgcc tcatcagctc ggaacggtcc
     2461 atctcgtgac ccaatgtacg cccatgcgcc cctatcggcg aatgcgctcg agaggaggac
     2521 ccgcgggccc gctgatcgga cgctcgtccg gcccggaccg ccgccgcccg ctccgcggca
     2581 cctttggcgc ccgcggctcg gaccggcgcg cgtgcgccac gacgatgccg atcaccgacg
     2641 cgaggagccc cagcgccacg cagccgagca gcgcactcgc gagccccacg tggtggccgg
     2701 tcacgagcag gaacaggtac caggcgccgt cgagcggacc cagccggagg tgctgggcgt
     2761 aggtgaacac gatcagggcc gcgggcagca gcccgacgag cgccatccac gcgcgggtgc
     2821 gcgccggcac gccggagagc accgcgagca tccaggcgtg cagcgggggg atcagcacca
     2881 ggcccgtgta ggggttgatc gcccacgcgg tgagcgcgag ggcgcacagc gccagcgtca
     2941 ccaccaccgc ggctccgggc gcggcggggt ccgggcgcgg gcgctcgcca cgcccgagcg
     3001 acgtgcgcgt gacgatccag gccagcccga tcgtgagcgc caccgcggca agcacgccga
     3061 tcgcagagcc gtgggcctcc gccgcactcg gttcgagcgg cgcgggtggc gcgtcgtcgg
     3121 ccagcccgac cagcacgagc agctcggcga gcgcgaggcc gaccgcgaac ggggcgatcc
     3181 ccgcggcgac ccatggcagc cacggcagca cgtcctcgcc gcgccggcgc acacgcgcga
     3241 gcgcgtcgac tcccgcgaac agcaccggga ggatcagcgt gagcgccagg agggagatcg
     3301 accacgcggg cgccacgcgt ccggcgagcg tgatgtacga gcgcggcccg cgctccggca
     3361 gccgcgcctg ctcgtcgagc gtgaacgtga accgcagcgc cgcgcggccg aactcctcgt
     3421 agcgctcggt ggcgaggtcc gcgaggtcgc gctggtcgcg cgggggcgcc aattcgccgc
     3481 tggcggacat gcggatcgcg gtgaggccct cctccatcag caccgcctgc gggccgagcc
     3541 cgaccgggaa cgcgagccgc ccgagttgcg tgagcgggct gccgaagccg ccgccgtcct
     3601 cacccacctc gttgcgcagc gcgttcgccg cggtgcggcg cagcccgatg ctggcccgct
     3661 cgtcgctgtt ggaggagccg atcagcatcg gcccgcgcgg ccgccgcgcg cccaggttcg
     3721 acaggacgat caccgcgtcg accggcccgg ggccggcgac ggtccgcgcc aggcggcgcg
     3781 cgcccgcgtg cccgcgcccc gcgccgtcga ccgaggcgag cacgatcgtc ttcttcgtgg
     3841 cgcgtccctc gagcacgcgc ccgaactcga tgagcgccgc cgtgtcggcc gcgctgccgg
     3901 tcgcgtcggg caccgacagc gcgtcgcgcg agacgagcac caccacctgc cgccgcgaca
     3961 ggcccgggcg ccggccgagc acgttcacga gccgcacgcc ctcgtcctcg aagcggtcca
     4021 ccgtggtggt caggccgaag cgccggaacg caccggccac gcgctgcgcg accgcggcgt
     4081 cgcccggagt cccggcgcgg cggtccggcg tccgctccac gatcgcgcgc acggtctccg
     4141 ccgcgctggc gccctcgaac aacacgtcgg ccggagcggc ctgcggcaac ggccgcggct
     4201 ggctctcgag cgcgaacatc gtgacgatgg cggccagcag cgccgggagg aacgccgcac
     4261 ggtagatgcg cggttcgatc acgattccgg gcccgagatg accgctccaa cctacaatct
     4321 cgcgggttag ggaggacggt atgtcaaccg aggcccccag gatcctcctg gtggacgacg
     4381 agcagtcggt tcagaccctg ctctcgtacc ccctgcgcaa agaggggtac gacgtcgttc
     4441 ccgcctcgac cggccgcgag gcgctcgagc gcttccgcga cgcgcacttc gacctcgtgg
     4501 tgctcgacgt gatgctgccc gaggtcgacg gcttcgacgt gtgcaaggag ctccgcgcga
     4561 agagctccgt cccgatcatc atgctcaccg cgaagaccga ggaggtcgac aaggtcctcg
     4621 gcctcgagct cggcgccgac gactacatca ccaagccgtt ctcgatgcgg gagttctcga
     4681 gtcgcgtcaa ggccgtgctg cgtcgcgccg gcctggcccg caacggcgac gcggtcgagg
     4741 agccggtcga gcacggcgag ctgcggatcg acttcgccaa gcgctctgtc gagctgcgcg
     4801 gcgagccggt ccggctgacc tatgtcgagt tcgagatcct gtccgcgctg gtcagcagcc
     4861 cggggcgcgt gttcagccgc gggatgctgc tcgaacggct ctggggcgac tccgcctacc
     4921 gcgacccgcg cacgatcgac gtccacatcc ggcacctgcg cgagaagctc gagcgcgacg
     4981 cgcgcgagcc cgagctgatc ttcaccgtgc gcggggtggg ctaccacttc cgcgaccgct
     5041 agaggaccgg aaccggtcct ctgggcgtac gctgatcgca tgcggctgcg gtcgctgcag
     5101 aacaagctcg cgcttctctt cctcgcgatc acggcgctgg cgttcgcggc tgcgatcttc
     5161 gtggtgctgc cgcgcctcga gacgcagctc caggacgagc gcgtccggga tgtcgagcgc
     5221 gtggcggagt cgggaacacc gcggattcag cgggtggtcg ggcagaacat cgcggccgga
     5281 cagctcgacg agctggtgcg cgctgtcgcc gacgccggct cggtacgggt cacgctgctc
     5341 ggcacccagc gctcgagcac cggcggcacc gacttctacc cgctgtccga ctcgaacgcc
     5401 gagcgccgcg tctcaccgga ctacgacctc gcggcggagg cgtaccgcgc caagcggccc
     5461 ggcgagacct tccgccgccg cgccgacagc gagggacggg tcgcccaggc gatcttctac
     5521 cgcggtcagc cggtctggat cgcgatctac tcgcgcccgt tcgaggacgt gagcgactcc
     5581 gtcgcgctcg ttcgcaaccg gctgatcctc gccgccgtgg tggcgctgct cgtggcgctg
     5641 atcggcgggt tcctcgtcgc ccgcgcgctc gcgcgccgag ttcggcggct cgagcacgcg
     5701 gcgcaggagg tcaccgccgg ccgtccggtg ccgccgctgc cggtggactc gaacgacgag
     5761 ctcggcgagc tcacgcgcgc gttcaacgag atgcagcgcc agctcgcggt ggtggaccgc
     5821 gcgcgcaagg acttcatcgc aaacgcctcg cacgagctgc gtacgccgat cttctcgctc
     5881 gccgggttcg tcgagctgct ggaggacgac cagctcgacg aggccacgcg ccggcacttc
     5941 ctcgagacca tgagggagca ggtcgagcgc ctccagaagc tggccgtgga cctgctcgac
     6001 ctctcgcgga tcgacgccgg gtcgctccag ctcgcccccg aggacgttga cctccgcgag
     6061 ctcgcccgcg atgtcgccct ggagttcgcg ccggcgctcg accgccatcg caccgagctc
     6121 gagatgctgc tgcccgaggg acccgtcgag gcggtctgcg accgcgggcg gacggttcag
     6181 atccttcgta tcctgctcga caatgccctc cgtcacacgc cggagggcac cgacgtcacc
     6241 gtgaccgcag ggaggcacaa cggcgcggcc gagctcgcgg tcgccgacac cggcccgggc
     6301 atcgaccccg cggcgcgcca gcagctcttc gagcgcttct acacggccga cgccgcccgc
     6361 ggctcgggcc tcggcctggc gatcgcctgc gagctcgccg agcggatgga gggccgcatc
     6421 gagctcgatg cggcacccgg ccggacggtc ttcaagctgg cgctgccggc cagcagcgag
     6481 cccgagcccg aggtctcgtg aggggcatag cgcgcgtagg gacggcggcg ctcgccgcgg
     6541 cgtcgctggc gctgggtgcc ggcgcgtgca cggacgacgg cgacggcggc tcgcgcgggg
     6601 actatcagga ggcctcgccg ccgaccgagc gcacccaggt gcgggtgccc gagggcctcg
     6661 accgcgggcc gttcgacgcg cgccggatct accgcgcgct ggcgccgggc gtggtcaccg
     6721 tgctcgccca gatcgacggc ggcgtggtcg gcgaccagca gggcatcggc tccggcttcg
     6781 tgctcgacgg cgacggctac gtcgcgacca acgcgcacgt gatccgcacc gacccgcccg
     6841 acctcgagcg cgcggacgcg gtgtacgtgg agctggccga cggcaatcgc gtcgaggcga
     6901 aggtggtggg ggacgacctg ttcagcgacg tcgcgctgct gaaggtcgat ccgcgcgggc
     6961 tcacgctcac gccgctcgag ctcggacggt ccagcgacct gacggtcggc gagccggtgg
     7021 cggcgatcgg cagtccgttc ggcgagccgc agtcgctgtc ggtgggcgtg atctccgcgc
     7081 tcgaccgctc gatccagtcg ctgacgagct tcaagaccga ggacgcgatc cagaccgacg
     7141 ccgccatcaa ccacggcaac tcgggcggcc cgctgatcgg ccgcgacggg cgcgtgatcg
     7201 gcatcaacgc gcagatccag tcgaccggcg gcggcggcga gggcgtcggc ttcgccgtgt
     7261 cggtggacgt ggtccggcgc tcgctcgcac agctacgcga ggacggcagc gccgagtacg
     7321 cgtacgcggg gatcacgtcg caggacctct acccgcagct cgcgcggcgg ctcggctacg
     7381 acgtgggtcg cggtgcgctc gtgcgcggcg tcgagggcgg cgggccggcg gacgacgccg
     7441 ggatcgaggc gggcgacgag cggatcgagt tccagggcca gcgcgacatc ccgcgcggcg
     7501 gcgacgtgat cgtcgcgatc gacggtcatc cggtgcgcgg gtcgagcgac atcgccgagg
     7561 cgatcgggcg gctgcggccg ggcgaccgcg tggtgttcga cgtcgcgcgc ggtggcgagc
     7621 gccgcaaggt cgaggtcgag ctcggcgcac gcgacgcgac gcccgcgggc ggctagcgcc
     7681 gtggcgagtg cggagctggt gcgcgagctg gcgctcgcgc tgcgcgagcg ggtgctcccg
     7741 ctgctcggct cgcacgccgg gcgggcgcac gcgggcgacg gcgccggcgg cgacgtgacc
     7801 ttcgccatcg acgaggaggc cgagagctac ctcgagtcgt tcctggccga gcgcgcgccc
     7861 gataccgcct tctactccga ggaccgcggc ctcgtggtgc cggcgggcgg ccgcgcgacc
     7921 gaggtgctcg tggtggaccc gatcgacggc acgcgcccgg cgatggcggg gctcgagtcg
     7981 tgctgcgtgt ccgtggccgc cgcgccgctg cgcgacggcg tccggatggg cgacgtgacc
     8041 gccggctgcg tcgtcgagat cccgagcggc acgatcttcc tcgccgagcg cggccgcggg
     8101 ctgatcgagg ggccgccggt ggtcctctcg ccgaacgacc ggctcgaccg catgttctgg
     8161 acctacggct ttcgcggccg cccggcgcgg gcgctcaccg aggtgctggc cgagctgatc
     8221 gactcgtcct cggtgggcgg tgcgacgttc gacctcgggt cggccgcctt cgacatgacc
     8281 cgcgtgatca ccggacagct cgacgcgtac gtggagcccg gtccgctgct cgtcgacgag
     8341 gtgcccggga tgcgcgccga gttcgagcgc gtgggcggcg gcgccgtgct gaacaactcg
     8401 ccgtacgacc tcgccggggc ggcgctctgc gtcaccgagg ccggcgccgt cctgaccgac
     8461 gcgcgcggca ggtcgctcga cgatcgcccg ctgctcggct ccggcgccga cttccagatg
     8521 tcggtggtga ccagctcgaa ccccgctctg cacgagctga tcctcgccgc cgtggacgct
     8581 ggtgtggcac gggtgggctc gggtagggtt gacccagccc tctgagcccg atggaagccg
     8641 cagcccccaa caggaccgtc acgctggccg ttccggccaa gccggactac gtcgtgctgg
     8701 cgcggctcgc gctgtcggcc gtctgccgga tcgccccgct cgagctcgag gcggccgccg
     8761 acctcaagct cgccatcacc gaggccgcga ccttcctgat gggcggcgac cgccgcagcg
     8821 aggcccgcga ggacgaggag agcgagcaga tggtgttcgc gttcgacctc cgcgacgacg
     8881 agatcgaggt ccgggtgcgc ggaaccgagc agcgcacgtt ctccgaggag gagcgcgagc
     8941 tgagccgggc gatcatcgac gcgaccgtgg acgattgccg ttacgaggac ggcacgatgg
     9001 tgctcaagaa gcgactcggc ctccccggcg agtaggctgc cgcctcttcg agcgaaggag
     9061 cgcctgttgc tgcagccggt aggggtgggg cacaaggccc tggcggacta cacgcacctc
     9121 gtggggcgtc cgctcataga ggagatcagg acgctctccg accagatggc gggcctacgg
     9181 gtcctgcacc tgtccgcgac cgccttcgga ggcggcgtct cggagatcct gtacacgctg
     9241 atcccgctga tgcgcgacgt gggcatcgac gccgagtggc acgtgatcat cggccgcgag
     9301 gagttctaca acgccacgaa gctcatgcac aacgcgctcc aggggcaccc gctcgacctc
     9361 gacccgcacg agtggaaggt ctacgaggag tacaacgaga tcaacgcgcg cgagctgacg
     9421 ggcgactggg acgtgatcat cgtccacgac ccgcagccgc tcgcggtgcg ccagcactgc
     9481 ccggagaagt cgaagaactg gatctggcgc tgccacatcg acctctcgac cccgcacccg
     9541 cccacggtcg cgcggctcgt ccccctgatc gccgagtacg acctcggctc ggtctggcac
     9601 ctccagcagt acgtcccggc cgggctgaac ggcgccggcc cggagcacat catcccgccg
     9661 gcgatcgacc cgctgtcgcc gaagaacatg gcgctgtcgc ccgaggacgc ggcgttcgtg
     9721 tgcgaccagt tcggcgtgga cgtggaccgg ccgctcatgt gccaggtctc gcgcttcgac
     9781 ccgtggaagg acccgcacgg ggtgatcgac gcctaccgga tcgtcaagga ggaggtcccg
     9841 gaggtacagc tcgcgctggt cggctcgatg gccaccgacg accccgaggg ctgggagttc
     9901 ttcaactcga ccctcgagta cgcgggcggc gacccggaca tcaagatcct caacaacctg
     9961 aaccaggtcg gctcgatcga ggtgaacgcc ttccagtcgc aggcggaggt cgtgatccag
    10021 aagtccaccc gcgagggctt cggcctaacg gtctcggagg cgctctggaa ggcgcgtccg
    10081 ttcatcggcg gcgacgtcgg cggcatcccg cttcaggtcc aggacgggac gacgggctac
    10141 ctcgtctcgt cgcccgagga atgcgcggat cggtccctgc gaatcctgcg cgagccggag
    10201 ttgggtaagc agctcggccg tgcgggtaag gaacatgtgc ggcagcactt cctcacgccg
    10261 cgcctccttc gcgactggct gcgcatcttc tcggacctga agtgacctcg gacacccctc
    10321 tcgtactcgt ctcgaatcgc ggcccggcca ccttcgagcc ggacgacgcg ggcgagctcc
    10381 agccctcgcg gggcgggggc ggcctggtca ccgcgctcaa ggggctcgtg gaccatcgcg
    10441 acgccctctg gatcgcctcg acgctgaccg agggcgacag cgagatgagc cgccgctcgg
    10501 gcgggcgcag cttcgagtgc gacgtggacg agacgaccta ccggctgcgc ctggtcgaca
    10561 gcgacccgga cgcctacgac cgcttctaca acgtgatcgc gaacccgatg ctgtggttca
    10621 tccagcacta cctctgggac ctctcgaacg cgccggacat cagccgcgac gaggtggtcg
    10681 cctacgagga gggttaccgg gtcgtcaacg aggacctggc gcgcgcggtg gtcgaggagg
    10741 tcggggacct cggtgacgcg gtcgtcatgc tccacgacta ccacctctac acggcgccga
    10801 aggtgatccg cgcggagcgc ccggatctct tcctccacca cttcatccac atcccttgga
    10861 cccagccgga cgcgtggcgg gtactgccgt cggacatgcg cgaggacatc tacgagggac
    10921 tgctcgccaa cgacatcgtc ggcttccaca cgcaggccta ccggcgcaac ttcctgctct
    10981 gctgccgcga gctgttcggg ctcgacgtgg acatggaggc gggcgtggtg cgccacgacg
    11041 agcgcgagac ctgggtccgc cactacccgc tgccgatctc ggcgtcgagc ttccgcaaga
    11101 ccgctcagcg gccggcggtg cacggctacg agcgcgaggt cctgcgccgc cggcgcgagc
    11161 acctgatcct gcgcgtcgac cgcgccgacc tgtcgaagaa cgtgctgcgc gggttcaccg
    11221 cgttcgacct gttcctcgac cagcacccgg agttccgcga ggaggtcacg ttcatcgcgc
    11281 agatgatgcc ctcgcgccaa gacgtgcccg agtacgcgga gtacctcgag aagatcgagg
    11341 cgctggtggc ggtcgtgaac caccgccacg ggacgaccga ctggatgccg atcgacctgc
    11401 acctggtgga caacgtcgag caggcgatcg cgcgctacaa gcactacgac ctgctgatgg
    11461 tcaacgcgat gttcgacggc atgaacctgg tggcgaagga ggggccgctg gtgaacgagc
    11521 gccacggggt ctcgctgctg tcggagaaca ccggcgcgca cgaggagctc ggcgagttcg
    11581 cgctgtcggt gaatccgttc gacgtgcagg agcaggccga cgcgatccac cgcgcgctga
    11641 cgatgtccgc cgaggagcgc tcgtggcggg cggagggcct gaagaagatc atctcctcgc
    11701 gcgacccggg cgactgggtg gacgatcagc tcgaggacat cgacgcgaag cgctcgggag
    11761 cccggctcgg cccgggcggc gtggtggcgg cggcgtagcc ggccgccgcg gtggcctggc
    11821 gcccgcgggc tagaagagcc agcagcgccg gcgcgcggcg agctcttcgt tgcgcttgac
    11881 cacgtcgatg gcggcctgtc gggcggcccg gttccgcgcc actctcgcgt cgaacctgcg
    11941 ccgccactca gaccattcgg cctccgactt gtggaccgcg gcccacgtca gccggccggg
    12001 tcgatcacgg ggtgtttctt cgctcatctg acctccgctg ctcattgcat cgtaccccca
    12061 tcgccgttgc ccgacgggcc gagcaggtca cgctcgaggc gcagcagccg cgatctcgtc
    12121 tcctcctcga ggtggctcag gtcctcctcg agatcggcga tcgatgcgct gttctcggcg
    12181 aaggcgaggc ccgtaccgcc cgggagctgc gcggaggtga cgatcgcgcc ggcccacagc
    12241 ctccctccga gcccgcccag caccatgacg aggcccgcaa cgaggccagc tttcgtgagc
    12301 gcggcggcca gggcggtccc cctctcgacc tcgctcacga ggaccgcgag cgcgagcgcg
    12361 ccggccacga ccacgatcac gcgggcggcg aacggcggtg tcacaggaac tccggcgcgc
    12421 gtggggctgg cgtccagtga cgtggcgtgg ttgccggtgg aggagccgcg ggcggtgcgt
    12481 gcggtgtggt ggggagcggc ggaggggccg ccgagccggg tgccgggcgc cgccgcgggc
    12541 ggcgcgtgct ccggcgggca cgcccgcacg agccgagccg ccgtcgcctg gcgcgcttgc
    12601 gccgtgtggc gctccgcttc gcccgcgggc ggggggtctc ggtgccggtg gagctgcgcc
    12661 gcggctcggg gtcgcgatct cgctggccgg cgtccggagc cggcgtgctg ccagatgcgg
    12721 cgggcggcac cgcagaggcc ggcggttccc ccggcacccg cgggggcacg cccgccggcg
    12781 gctccaccgg cagccgcggc agctccgact gaccgccgcc gcacccacgc ggccccgccg
    12841 cgatcagcag cccggccccg acgatcgccg cgagtcgcgc cacgttcccc cagcggacgc
    12901 gttgcagcaa cgcgctcgcc cggctctctc gatatcgccc catgcgggca taagcgccga
    12961 gcggcaggaa ctcgccccgc tgatatcttc gcccgcgtgc cgaacctgta cgacctcatg
    13021 gtcatgctcg atcccgacgc gcccgaggag cggcgcacgg agatcctcag caacatcgag
    13081 acgatggtcg cgaacggcgg cgggaccgtc gtgggccacc acgactgggg cgtccgccgg
    13141 atcgccttcg agatcgacca ccgccccgag gccgcttacc acctgtacca gttcgagagt
    13201 ggctcgaacg agacgctcga gcgcctgaac cacaccctgc ggatcgccga cggcgtcctg
    13261 cggttcagga tcatcaagct caagccgggc tcgccgcctc cgccggcccc gcgccaggag
    13321 gctccgcgcc cgccgcggga gcgtccgcag gagacccgcg tggccgcccg cgccgccgcg
    13381 gacgcggacc agcccgaccg cgaagacgcc tagcggcgcc gctccgttac aggagcgcaa
    13441 acaacccgca cgttccggtt acaacacggg agatcccgcc cggcacgtcc attcccgcat
    13501 gtaccctcgc cggagcgatt tcatcgaacg tcagcagagg caaggagctt ccgagagatg
    13561 gcagcaacaa acatcaaccg ggtcgtcatg accggcaacc tcacgcgtga tccggagctg
    13621 cgcagcacca acagcggcac cccggtctgt ggtctgcgga tcgcctgcaa cacgcgccgc
    13681 aaggacggct ccggcaactg ggtcgacaag cccaactact tcgacgtgac cgtctggggc
    13741 gctcagggcg agaactgctc caactacctc tccaagggcc gcccggtcgc gatcgacggc
    13801 cgcctcgagt ggcgcgagtt caccgacaag gacggcaaca accgccaggc ggtggagatc
    13861 gtcgccgact cggtccagtt cctcggctcg cgcgagggtg gcgagaacgg cggtcagaac
    13921 ggcggccgct tcacgccgca gagcgacgtg ccgaccaaca cggacgactt cgcgccggct
    13981 ccggtgggtg gcggttcgtc cgccgactcc gacgacatcc cgttctagcc cgttgcaaac
    14041 cgtcgcccgc ccgcgtcctc ggtcgctcgc gtgctgttcg cacgcgttgc tccctgcgtc
    14101 cttggcatgc tcggttttca cgggctcctg atcacgggta tggctacact cgccgctgac
    14161 ttcggacgac gaaaaggtga ctacgtagtc aatggcgaaa gcacgcggcc ggcagcagac
    14221 gcgccggcga gacagaggga agcccgccac cggcggtcgc aggaagccat gtcagttctg
    14281 caaggacaag gtggacgtgg tggactacaa ggacgtcgcc accctgcggc gggcgatgag
    14341 cgagaagggc aagatccggt cggcccggat cacggggtcg tgccggcggc atcagcgcca
    14401 gctcgcacgt gccgtgaagc gcgcgcggga gttgggcctg ctcccgtacg tctcagatcg
    14461 ctagcgaggc tgttctccta atggctcagg cgatcctcct cgaggacgtg gacacgctgg
    14521 gggagcgcgg cgagatcgtc gacgtctccc cgggctacct gcgcaactac ctcctgccgc
    14581 gcaagctcgc gcacgccgcc acgcccggcg ccctcgccga ggccaagcgc cgcatggagg
    14641 cggccgagcg cgcggcacgc gaggcggagg agcgcgcgca ggagaacgcc gccctgctct
    14701 cgcgcaccgt gctcacgatc tcgcaccccg ccggcgagga cggccggctc ttcggacagg
    14761 tcacggcgca ggagatcgcc gacgccatcc gccaggcccg cagcctcaag ctcgaccgcc
    14821 gcaagatcca gctcgaggaa ccgatcaaga ccaccggcac tcacatggtc gaggtcgagg
    14881 tgcacgacgg tgtgatggcc cgcgtcaaaa caatcgtcac cgcggctgga tgatcctatg
    14941 cgtcactgcg gcgtagtccg ccagcccgag taggacccc
//
LOCUS       DASVPL010000129         5286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_211157, whole genome shotgun sequence.
ACCESSION   DASVPL010000129 DASVPL010000000
VERSION     DASVPL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211157"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..292)
                     /locus_tag="VF517_06620"
     tRNA            complement(219..292)
                     /locus_tag="VF517_06620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Val,seq:gac)
     gene            complement(330..1325)
                     /locus_tag="VF517_06625"
     CDS             complement(330..1325)
                     /locus_tag="VF517_06625"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8052648.1"
                     /translation="MRYLITGGSGYIGTRLVERLSEREETERIVVADIRPPASHRPKV
                     AFERLDVRDAARARELMDAHRPDVVIHLAFVLNPIHDQEVEYDIDVGGTQNVLRAASE
                     AGVGHVMITSSATAYGAFPDNPVPIAEDWPVRGVPSFPYACHKTEADRLAQLWALEHP
                     DRTMTIVRPCIVFGPNVDNYIVRLWTEQPFQVDLGVPPQPLQFVHEDDLVDALERLLH
                     GGHGGAFNVAGEGTMLTTECADAIGMKRRRVPLGAYRRLVAAMWKLRQAETPPGNIEF
                     VIHPWVVSTEKLERTTGWSPRHTSRATFEITMRARGALPADGAGVKPELAAPVGA"
     gene            complement(1433..2005)
                     /locus_tag="VF517_06630"
     CDS             complement(1433..2005)
                     /locus_tag="VF517_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052649.1"
                     /translation="MTKQITTVALLAGALVVPATAAAEEPSQANFKNAAKYCKALKAE
                     AGDANFKAMFGGKSNAYGKCVSKAAKRDAAQEKTAKTNAAKQCKTEQAADSAAFTTKY
                     GTGNGKNAYGKCVSQKAKENKADADKQEKEDVNSAEACRTEQKQDAAAFKTKYGTNES
                     KSNAFGKCVSQKSKEKNAEPAPTPPAGSTA"
     gene            2118..2486
                     /locus_tag="VF517_06635"
     CDS             2118..2486
                     /locus_tag="VF517_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052650.1"
                     /translation="MSTKHPTERGGQSRGVFDRIAEAGSNFTSSPAFFALCVLTIATW
                     IAAYALGLGTNFKHIAGEAMAAVSLLTLALLKNSELRAEQAIQEKLDAIAAALLEQRE
                     GEGEEAAEDLRRAIGMHEET"
     gene            complement(2588..3031)
                     /locus_tag="VF517_06640"
     CDS             complement(2588..3031)
                     /locus_tag="VF517_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052651.1"
                     /translation="MDTGVIIGIAVAAAVVIALLVIISRAAKKRRMHARREQAHELRQ
                     DAQSRELRASRQQAEADQRSARAKQAAAEAEEKAALARREEAVAQERAQGAERERHFA
                     RERHGEAQSIDPDASDDDGREPVSAGNGNGNGRRARQRAGGRRRS"
     gene            complement(3128..4417)
                     /locus_tag="VF517_06645"
     CDS             complement(3128..4417)
                     /locus_tag="VF517_06645"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX8052652.1"
                     /translation="MTLTTTDAQRKRAGDAADALWMNAQAEIARVGALLGLGDGTVDM
                     LTQPRRSVEVAVPIRLDTGATEVFAGYRVQHSLTRGPAKGGVRYHPHVSLTETKALAM
                     SMTWKCALVNIPYGGGKGGVRCDPGQLSSGELERITRRYASEIMPLIGPGRDVLAPDI
                     GTGEREMAWIMDTYNAAHGAVAGSPVTGRPVIVGGSEGRRRATGYGVVECVKLGVGLL
                     GMDAPVRVVVSGFGEVGRVAAELLSSHGGFSVVAVGDVTGGRHDPRGLDVAAIGAHCD
                     AGGALVEAPGGEPVDVGELIELPCDVLVPAAVAGVVNEQNAEGVRAALVVEGANHPTT
                     AGAEAILGERGRTVVPDILANAGGVIASHLESVQDTHGSAWRARETYEGVEQRLRGAF
                     GAVCELAGERGVGMREAALCLAVERVAATHRTLGLYP"
     gene            4589..>5286
                     /locus_tag="VF517_06650"
     CDS             4589..>5286
                     /locus_tag="VF517_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052653.1"
                     /translation="MTRKVTLMALALAALLVPAAGATAAPPPAELRAASGQQITAAAF
                     APLTKTSARRLALKLARDVARDRDVRWWQLSAPIKVRSSRITFAYADRTRANTYCTST
                     IAVEQPSRRLRRTSFIGGRCRGVPAEALAVERETSRFIRAAHARQPDLRDSIDSYNEQ
                     LAECEELRVPRNRLAQVRRIFMAHEFAAAMGPIEAEIGEFVSALDALRLQRPELAAAP
                     RGWEAMNAAVNLIR"
BASE COUNT          676 a         1934 c         1873 g          803 t
ORIGIN      
        1 gccgacggcg cgcaggcccg cgaccgcgag ccaggccagg aggccgagcc acgcgaccgt
       61 cgagacgaac gacacgacca cgaacgcgac cagtgtcacg cgctcgcgga gctcctggtg
      121 cggcccgtcc gtggacggaa cgctgcctgc ggcggtctcg ctcatcgcgg gcagcatcgg
      181 cagcgatggc ggcgcgcatg aatggcgcac gcgcgggatg ggccgtgctg gactcgaacc
      241 agcgaccccc tgcttgtcga gcaggtgctc tagccagctg agctaacggc ccgcagagcg
      301 gcgaggatag ccgccgtgct ggcgcccggc tacgcgccca caggcgcggc cagctcgggc
      361 ttcacgcccg cgccgtcggc cggcagcgcg ccccgcgcgc gcatggtgat ctcgaacgtc
      421 gcgcggctcg tgtggcgcgg cgaccagccg gtcgtccgct cgagcttctc ggtcgagacg
      481 acccacggat ggatcacgaa ctcgatgttc cccggcggcg tctccgcctg gcgcagcttc
      541 cacatcgccg ccacgagccg ccggtaggcc ccgagcggga cgcgcctgcg cttcatcccg
      601 atcgcgtcgg cgcactcggt cgtgagcatc gtgccctcgc cggccacgtt gaacgcgccg
      661 ccgtggccgc cgtggagcag gcgctcgagc gcgtcgacca ggtcgtcctc gtgcacgaac
      721 tggagcggct gcgggggcac gccgaggtcg acctggaacg gctgctcggt ccagagccgg
      781 acgatgtagt tgtccacgtt cggcccgaac acgatgcacg gccggacgat cgtcatcgtg
      841 cggtccgggt gctcgagggc ccagagctgc gcgagccggt cggcctcggt cttgtggcag
      901 gcgtagggga agctcggcac gccgcgcacg ggccagtcct cggcaatcgg aaccgggttg
      961 tcggggaacg cgccgtaggc ggtggcggag ctcgtgatca tcacgtggcc gacgcccgcc
     1021 tccgaggccg cgcgcaggac gttctgggtc ccgcccacgt cgatgtcgta ctcgacctcc
     1081 tggtcgtgga tcgggttgag cacgaacgcg aggtggatca ccacgtcggg ccggtgggcg
     1141 tccatcagct cgcgcgcgcg ggcggcgtcg cgcacgtcga ggcgctcgaa cgcgaccttc
     1201 ggccggtgcg acgcgggcgg ccggatgtcg gccaccacga tccgctcggt ctcctcgcgc
     1261 tcggacaggc gctcgacgag ccgcgtcccg atgtagcccg agccgccggt gatcaggtag
     1321 cgcacaggcg cactgtacgc cccggaacga cgaaggggcc ccgtggaggg gccccttcgc
     1381 tctggtcgta cgcggctcgc gaggggtcga gccgcggcac gtgttggggt acttacgccg
     1441 tcgagcctgc cgggggggtc ggggcgggct cagcgttctt ctccttcgac ttctgcgaga
     1501 cgcacttgcc gaacgcgttg ctcttgctct cgttcgtgcc gtacttcgtc ttgaaggcgg
     1561 cggcgtcctg cttctgctcg gtgcggcagg cctcggcgga gttgacgtcc tccttctcct
     1621 gcttgtccgc gtcggccttg ttctccttgg ccttctgcga gacgcacttg ccgtaggcgt
     1681 tcttgccgtt gccggtgccg tacttggtcg tgaaggcggc cgaatccgcg gcctgctcgg
     1741 tcttgcactg cttggcggcg ttggtcttcg ccgtcttctc ctgggcggcg tcgcgcttgg
     1801 cggccttcga cacgcacttg ccgtaggcgt tgctcttgcc accgaacatg gccttgaagt
     1861 tggcgtcgcc ggcttcggcc ttgagggcct tgcagtactt cgctgcgttc ttgaagttcg
     1921 cctgcgacgg ctcctcggcg gcggccgtag ccggaacgac gagggcgcct gccagcagcg
     1981 cgacggtcgt gatttgcttg gtcatgtgcg agttctccct gcggttgtgg acggtccgtg
     2041 cccggggttt caagcgttgg aacgcgggga ggggccggag gttgagtagt tgcggcggcg
     2101 ggtaacccgc gcggaccatg agcaccaagc acccgaccga gcgcggcggc cagtcgcgcg
     2161 gcgtcttcga ccgcatcgcc gaggccggct cgaacttcac gagctcgccc gcgttcttcg
     2221 cgctctgcgt gctgacgatc gcgacgtgga tcgcggccta cgcgctcggg ctcggcacca
     2281 acttcaagca catcgccggg gaggcgatgg ccgcggtcag cctgctcacg ctggcgctcc
     2341 tgaagaactc cgagctgcgt gccgagcagg cgatccagga gaagctcgac gccatcgcgg
     2401 ccgcgctgct cgagcagcgc gagggcgagg gcgaggaggc ggccgaggat ctccgccgcg
     2461 ccatcgggat gcacgaagag acgtagctgg cgctcgagcg cggccagaac gccgagcgcg
     2521 cccccgtggg gacgcgctcg cgcgtttcag ctaatccgcc gtgcctacga gcggtgatcc
     2581 gcggtgccta cgagcggcgc cggccgccgg cacgctggcg cgcacgcctg ccgttgccgt
     2641 tgccattgcc cgcgctgacg ggctcgcgcc cgtcgtcgtc gctggcgtcg gggtcgatcg
     2701 actgcgcctc gccgtggcgc tcgcgcgcga aatgacgctc gcgctcggcg ccctgcgcgc
     2761 gctcctgggc gacggcctcc tcgcgacgcg cgagggcggc cttctcctcc gcctccgcgg
     2821 cggcctgctt cgcgcgcgcc gaccgctggt cggcctcggc ctgctgccgc gacgcgcgca
     2881 gctcgcgcga ctgcgcgtcc tggcgcagct cgtgcgcctg ctcgcgccgt gcgtgcatcc
     2941 tgcgcttctt cgcggccctg ctgatgatga ccagcagtgc gatcacaact gctgccgcta
     3001 ccgcgattcc gatgatcact ccggtgtcca tcgctctctc ctcgcgttgg ggtacatggg
     3061 tatgaacgcg ccaccggccg gatggttgga aggcttcgcc ggttcgtcgc ggcgcggcgc
     3121 gtcggcgcta cggatagagg ccgagcgtgc ggtgagtggc ggcgacgcgc tcgaccgcca
     3181 ggcacagcgc cgcctcgcgc attccgaccc cgcgctcgcc ggccagctcg cacaccgcgc
     3241 cgaatgctcc gcggaggcgc tgctccacgc cctcgtaggt ctcgcgcgca cgccaggcgc
     3301 tgccgtgcgt gtcctgcacc gactcgagat ggctcgcgat cacgccgccc gcgttcgcga
     3361 ggatgtcggg cacgaccgtg cggccgcgct cgccgaggat cgcctccgcc ccggccgtgg
     3421 tcggatgatt tgcgccctcc accacgagcg ctgcccgcac cccctcggcg ttctgctcgt
     3481 tcaccacgcc ggccacggcg gccgggacca gcacgtcgca cggcagctcg atcagctccc
     3541 ccacgtccac gggctccccg cccggggcct cgaccagcgc gcccccggcg tcgcagtggg
     3601 ccccgatcgc cgccacgtcg agcccgcgcg ggtcgtggcg cccgccggtc acgtcgccta
     3661 ccgccaccac gctgaagccg ccgtgcgagg agagcagctc cgcggccacg cggccgacct
     3721 cgccgaagcc cgacacgacc acccgcaccg gtgcgtccat ccccagcagg ccgacgccca
     3781 gcttcacgca ctcgaccacg ccgtagccgg tcgctcgccg gcgcccctcg gagcctccga
     3841 cgatcaccgg ccgccctgtc accggggagc cggccaccgc gccgtgggca gcgttgtagg
     3901 tgtccatgat ccacgccatc tcgcgctcgc cggtcccgat gtccggcgcg agcacgtcgc
     3961 gccccgggcc gatcaggggc atgatctcgc tcgcgtagcg gcgcgtgatc cgctcgagct
     4021 cgccggagga gagctggccg gggtcgcagc gcacgccgcc cttaccgccg ccgtacggga
     4081 tgttcacgag cgcgcacttc caggtcatgc tcatcgcgag cgccttcgtc tccgtgagcg
     4141 agacgtgcgg gtggtagcga acgccgccct tcgccgggcc gcgcgtgagg ctgtgctgca
     4201 cgcggtagcc cgcgaacacc tcggtcgcgc ccgtgtcgag ccggatcggc accgcgacct
     4261 cgaccgaccg tcgcggctgg gtcagcatgt ccaccgtgcc gtcgccgaga cccagcagcg
     4321 cgcccacgcg ggcgatctcc gcctgggcgt tcatccagag ggcgtccgcg gcgtcaccgg
     4381 cgcgcttgcg ctgagcgtcc gtcgtcgtaa gggtcatctc tccacctcgc gtcggggtac
     4441 tagctcgacc aacgcggcag gggtccgcgg gttggagggc gactccaggc tggcgggtcc
     4501 gcgcgtgccg acttgcgcga ccggaggtca cgcgcgttag atttccgcgc taccgagggg
     4561 gacgcatgac aaccaggagg ggcaactcat gaccaggaag gtcacactta tggcgctcgc
     4621 gctcgcggcg ctgctcgtac cggccgcagg ggcgaccgcc gcaccaccgc cggccgagct
     4681 ccgcgcggcg tccgggcagc agatcacggc ggccgcgttc gcaccgctga cgaagacctc
     4741 cgcacggcgg ctcgcactga agctcgcgcg cgatgtggcg cgcgatcgcg acgtgcgctg
     4801 gtggcagctg tcggcgccga tcaaggtgcg cagtagccgg atcacgttcg cgtacgccga
     4861 ccggaccagg gcgaacacct attgcacctc gaccatcgcg gtggagcaac cgtcgcggcg
     4921 gctgcggcgc acgtcgttca tcggcggccg ctgcaggggt gtaccggccg aggcactcgc
     4981 ggtggagcga gagaccagcc ggttcatccg cgccgcgcac gcccggcagc ccgacctccg
     5041 cgactcgatc gacagctaca acgagcagct cgccgaatgc gaggagctga gggtgccgcg
     5101 caaccggctc gcacaggttc gccggatctt catggcacac gagttcgcag cggccatggg
     5161 gccgatcgag gccgagatcg gcgagttcgt ctcggcgctc gacgcgcttc ggctccagcg
     5221 tcccgagctc gccgccgcgc cgcgcgggtg ggaggcgatg aacgctgcgg tgaacctgat
     5281 ccggca
//
LOCUS       DASVPL010000130        13856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_56105, whole genome shotgun sequence.
ACCESSION   DASVPL010000130 DASVPL010000000
VERSION     DASVPL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13856
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56105"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VF517_06655"
     CDS             <1..939
                     /locus_tag="VF517_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8052654.1"
                     /translation="AGYLAKLENKRTRFVTSRGLHPHSRAALATYARGYGATDEETPL
                     DENGATDIEALAAAVDDDTGAVFLQQPNFLGTVEDLGPLVEAAKRTGALVVCQVDAMT
                     LGILEPPGAYGVDIAVGEGQPLGNRLDFGGPSFGFFAAQERYLRKMPGRIAGETVDAE
                     GQRGFVLTLQTREQHIRREKATSNICTAQALNALAGVIYLSWLGRRGVVEIGELMLRR
                     TAYAREKLGLELVNPGPVVREFAVRVPDLDAAVKRARAQGINPGYRLGRDYPELEDGL
                     LIAITERRTREQIDRLAGVLSDSGTPASQRAKVHAA"
     gene            936..2504
                     /gene="gcvPB"
                     /locus_tag="VF517_06660"
     CDS             936..2504
                     /gene="gcvPB"
                     /locus_tag="VF517_06660"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX8052655.1"
                     /translation="MRAANVPAADPTVTIFERSRPGRRAFSAPETDVPETPIEELLPA
                     SAIRREPPALPEIAEPEIVRHYNNLSKKNFDLDTGFYPLGSCTMKYNPKLNERVAALP
                     GHAKLHPAQDPEYAQGALELMWNLERALAEVAGLPHVSLQPSAGSHGELAGLLLTRAY
                     HEDRGEQRHKVLTPDTAHGTNPATVTMAGYEVVKVGTAPDGGVDLDDLREKATDDVAC
                     LMLTNPNTLGLFDKNIEEIAGIVHEAGATLYYDGANLNAVVGVSRPGDMGFDIVHFNL
                     HKTFTQPHGGGGPGAGPIAVSERIQPYLPVPRVVQVKGSNGSEPHFDLEHEATQSIGR
                     LRGFQGNFGVFVRSYAYIRSVGGDGLRDVAETAVLNANYLKALLAEPGIGEYLPIAFD
                     RTCMHEFVLSGKGAKQELGIKTLDIAKRLLDFRVHPPTVYFPLLVDEALMVEPTETET
                     KETLDRFAEVVRELLAEAKEDPEIARNAPYTTPVRRLDEGGAARRPVVRQQPPPQDPP
                     GDPDDPEPVSRGSL"
     gene            2523..3521
                     /gene="trpS"
                     /locus_tag="VF517_06665"
     CDS             2523..3521
                     /gene="trpS"
                     /locus_tag="VF517_06665"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX8052656.1"
                     /translation="MRIFSGIQPTGQKHLGNYIGAIRQYVEGQDRGDPAIYCIVDLHA
                     MTAPFDPAELRSNVLDTTAILLAAGLEPEKGRCILFRQSDVREHSELSWLLTCLTPYG
                     DLQRMTQFKEKSASARELVSAGIFLYPVLQAADVLLYRPHEVPVGDDQRQHIELLREI
                     AVRFNSRFGDTLVVPDHRIPDVGGRVMDLQEPERKMSTTQGTEQGTLHIRDDDRTMTK
                     KVKSAVTDSGREVVRGPGKAGIANLIEIEAVLRGQSPDDVERRFEGSGYGDFKGAVAE
                     TVVSTLGPIRERYLELAADPARIEAVLADGAARARVLAEETMKDVRERMGVGPAGT"
     gene            3579..4301
                     /locus_tag="VF517_06670"
     CDS             3579..4301
                     /locus_tag="VF517_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX8052657.1"
                     /translation="MRAAALDLDLEVFQGPFDLLLALVLREEIELAEVELAEVVLSYV
                     EYLERTGELDLEATTEFLVLIAALLELKSRLMLPGEEDEGVEWEPGEAADELLARLLE
                     YSRHKRAAEYLRERLEAESGFRYRSAPIPPALRRVALDLAEKSYEPDALAEAIGGLLR
                     TPPPIDLHHMARPRVTVEMRLAHLRDLLRSRTRFSFDDAVEGADRVTQAVTIFALLEL
                     YKKGEADWEQSAPFEPITVVGR"
     gene            4298..4867
                     /gene="scpB"
                     /locus_tag="VF517_06675"
     CDS             4298..4867
                     /gene="scpB"
                     /locus_tag="VF517_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX8052658.1"
                     /translation="MNELARVIEALLFLSPEPLTSTELADAAESTEGEVVEALARLRE
                     HYAEGWRGVVLREVGGGFTLATDPVAERAARRLFSRPRTPPLTQAQSECLAIVAYLQP
                     VSRPEIARIRGVASESAVSTLLERGLIEEAGRSQFGAILYRSTDLFQRLFGLSGLDGL
                     PDPARFDPTPEEEGELRERLLKAGDQRSS"
     gene            4896..5522
                     /locus_tag="VF517_06680"
     CDS             4896..5522
                     /locus_tag="VF517_06680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8052659.1"
                     /translation="MLASWREFPAAERTAGEHAALTFDDGPDPDATPAVLDALDAAGI
                     RATFFVVGEQLMRHWAIAREVRARGHELALHGAAHPRHAELSPPAARDEVARGIGAFE
                     AAVGARPALFRPPYGRFSEHSYAACAKLGLEPVYWSAWGSDWEPIPPARIADLVVRDL
                     APGAIVLLHDSARYAARSSAEATAAAIAAVAAAAAEAGVRLGPVTGSP"
     gene            5627..7708
                     /locus_tag="VF517_06685"
     CDS             5627..7708
                     /locus_tag="VF517_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8052660.1"
                     /translation="MQAAVPEKPRRFLRAVQVLSFLALAGYALHVGVGLGGDGMEPFF
                     SDYVYNGLVLVATASCLVRAVRVRARRAAWACLGAGLLSWAFADLYNTFYLAKLEEPP
                     YPSLSDAFWLAFYPMTYVALVLLVRERMKEARASLWLDGIVAALAVCAVGEVLVFSPM
                     AQASLEGGLSTPIEVATDLAYPIGDMLMLSLVVVVFSLTAWRPGRAWTMIGLGLAAMA
                     AGDGVYLYQASQGTYVEGTILDAMWPAATLLVGFAAWEPSRRTGDEIDLSGWRMLVLP
                     VLFALPSLGVLVYDHWTEVDGAAVLLATVCMLAVIVRTAMTFGENMRMLTTSRREAHT
                     DALTGLGNRRRLMADLDREMELAGPHSPRALGLFDLDGFKRYNDNFGHPAGDALLARL
                     GRALREAVEPEARAYRLGGDEFCVLGAADANAAAALVATATAALSEQGQGFFVTASHG
                     LVLLPLEAQDASAAMQIADQRLYGQKGSRRSSAVGQQTRDVLLEVLHARKPDLHVHLH
                     EVAEVALVVGHRMKLSAEELDEMARAAELHDIGKMAVPDEILNKPGPLDDRETEFVRQ
                     HTLVGERILAAAPALEEVAKIVRSSHESYDGSGYPDGLVGERIPLAARIIAVCDAFHA
                     MTDKRPYQPAMTPAEAMAELRRCKGTRFDPAVVEALCAEIGAGGIPPRVRRLPDAAPA
                     PAPAAPPVEAR"
     gene            7705..8805
                     /locus_tag="VF517_06690"
     CDS             7705..8805
                     /locus_tag="VF517_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8052661.1"
                     /translation="MTRFRGVAVAAAAAALALPAGASAATVSSAGGAVTFTAASGESN
                     RLSVFEAGGTLLFEDAGVSSMTAGPGCTPAGAQRVVCAVHGDGAVAVQLGDGNDVVVS
                     QVPLPFGVHGGLGDDGIVLSAGNDAVDGGAGADTIDAGGGDDAVDGGAGADTLDAGGG
                     DDSLAGGAGVDSLAGGAGADTLRARDGAAEPLACGSETDAVEADHGDAVDSDCEVVDR
                     GETAQPTFAPVLPPDPTGGVGSAIEAPVAAISGASLHVTRAGLTQVRLRCPRTAFEGC
                     SGSIVVEPLGVAAPNSRLDVTGARRVPIRLGTARFRALAGAGITVPVRLEPRVWRRLA
                     ARPRAKVRITVTMANATGATTSVRTVRVRRAG"
     gene            complement(8832..9818)
                     /gene="rlmN"
                     /locus_tag="VF517_06695"
     CDS             complement(8832..9818)
                     /gene="rlmN"
                     /locus_tag="VF517_06695"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX8052662.1"
                     /translation="MDLNLLDDVLARAGEPAFRSKQIWEWTARGASSYEEMTNLPAAL
                     RERLAADVPLSSLELAREARSRDGTEKALFNTSDGRPIEAVLMRYRDGRRSLCLSSQS
                     GCPLTCTFCATGRMRFARNLTSSEILDQALHFRRIEPVDHAVFMGMGEPLMNLDNVLA
                     ACERLPDLGIASRRTAISTVGWIPGIELLTEDGPAVRLALSLHAADEALRSELMPVND
                     RYPLPDVLDACLRWSRTRRQPVFVEYLMLDGVNDRYEQAMALADVLKPREAFKVNLIP
                     YNPTDSDLRGSTRGAIDAFRATLEERGLRATVRLTRGRDIDAACGQLAATAA"
     gene            9845..10549
                     /locus_tag="VF517_06700"
     CDS             9845..10549
                     /locus_tag="VF517_06700"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX8052663.1"
                     /translation="MRLAKFLAHSGVASRRAAERLIASGRVSVGREVVTDPARDVDAD
                     SGVAVDGELVRVERRVVYAVNKPRGVVSTAADTHGRPTVVELLPAEARRLYPVGRLDA
                     DSTGLLLMTNDGELANLLTHPRYGVEKVYRAHVKPPRITERALKALREGVELDDGMAR
                     AARARSVSGGVLEISLREGRKRQVRRMCEAVGLTVVELERVRFGPLRLGGLRQGSFRR
                     LTGPEVEQLREAANGG"
     gene            10678..11043
                     /gene="aroH"
                     /locus_tag="VF517_06705"
     CDS             10678..11043
                     /gene="aroH"
                     /locus_tag="VF517_06705"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX8052664.1"
                     /translation="MRLRALRGAITVDGNERDAILEGTEELLREVMGRNELAAEDIVS
                     CVFTCTADLNAAFPAEAARRLGLGAVPLLCAREIDVPGALPRVIRLMAHCYAEEDRQA
                     QHVYLREAVELRRDLEGAQ"
     gene            11040..12137
                     /gene="hisC"
                     /locus_tag="VF517_06710"
     CDS             11040..12137
                     /gene="hisC"
                     /locus_tag="VF517_06710"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX8052665.1"
                     /translation="MSLEFTARLRDIPTYPAAATYEYGGELVKLASNETPYAPHAAVV
                     EAIQAQLGALNRYPDPEKGLLRRRIAERFETNPGRVAVGNGSCEILMAAGEALLEPGA
                     EIVYAWPSFSMYPHLAAASGARAIEVPLTDGGHHDLDAMAREVTAATRVVLVCNPNNP
                     TATALPVEEIDAFLASLPRHTAVILDEAYVEFSTMQDPDESLPLLAKHPNLVLLRTFS
                     KVYGLCGLRVGYGLCGSEDFRIAVDKVRQPFSVNALAQAAAIEALRHQDEVERRVERT
                     AIERLHVEDELEERGLWSSDSQANFSWFALGDRDEAAVLKGLAERGVIVRGGTDLGHA
                     GHIRVTYGTRSENDRFLAALDEALAAAPKSS"
     gene            complement(12134..12352)
                     /locus_tag="VF517_06715"
     CDS             complement(12134..12352)
                     /locus_tag="VF517_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052666.1"
                     /translation="MERTLWTDERIDDTVARIEARFEQVEARLDRVEARLDRLEARID
                     DLYRQAFNFAIVQFTALLGLFVTILLRT"
     gene            12697..13710
                     /gene="aroF"
                     /locus_tag="VF517_06720"
     CDS             12697..13710
                     /gene="aroF"
                     /locus_tag="VF517_06720"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX8052667.1"
                     /translation="MKEGAGPDEVDAVVRQVESTGATAHVSRGEIHTVVGAIGDREHI
                     ANLELDGFPGVDHVVPISRPYKLASLQFRKEQPSVLDIGGRKIGGPHFMTIAGPCTVE
                     DRETMLEAARIVRDSGAQLLRGGAYKPRTSPYSFSGLGVEGLRLLQEAKDETGLPITT
                     ELMDVRDLEDVLEVADVIQLGARNMQNYTLLTEVGRAGRPVLLKRGLANTLEEFLMSA
                     EYILKEGNENVILCERGERTFEPNYRFQLDLMAIPVLKEMTHLPIVVDPSHAAGKRSL
                     VPALSYAAAAAGADGIIVEIHPEPEAAVCDGPQALYAEEFPEYLRKLEQAAALAGKET
                     VRV"
BASE COUNT         1950 a         5072 c         4995 g         1839 t
ORIGIN      
        1 gccgggtacc tggcgaagct cgagaacaag cgcacgaggt tcgtcacctc gcgcggcctg
       61 cacccgcact cgcgcgctgc gctcgccacc tacgcgcgcg gctacggcgc caccgacgag
      121 gagacgccgc tcgacgagaa cggcgccacc gacatcgagg cgctagccgc cgccgtcgac
      181 gacgacaccg gcgcggtctt cctccagcag ccgaacttcc tgggcacggt cgaggacctc
      241 gggccgctgg tcgaggccgc caagcgcacc ggcgccctgg tggtctgcca ggtcgacgcg
      301 atgacgctcg gcatcctcga gccgcccggc gcgtacggcg tcgacatcgc ggtgggagaa
      361 ggccagccgc tcggcaaccg gctcgacttc ggcggcccgt cgttcggctt cttcgccgcc
      421 caggagcgct acctgcgcaa gatgccgggc cggatcgccg gcgagaccgt ggacgccgag
      481 ggccagcgcg ggttcgtgct caccctccag acgcgcgagc agcacatccg gcgcgagaag
      541 gcgacctcga acatctgcac cgcgcaggcg ctgaacgcgc tggccggcgt gatctacctg
      601 tcgtggctcg gccgccgcgg cgtggtcgag atcggcgagc tgatgctgcg ccggaccgcc
      661 tacgcgcgcg agaagctcgg gctcgagctc gtcaaccccg ggccggtcgt gcgtgaattc
      721 gccgtgcggg tgccggacct cgacgccgcg gtgaagcgcg cccgggcgca gggcatcaac
      781 cccgggtacc gcctcggtcg cgactatccg gagctcgagg acgggctcct gatcgccatc
      841 accgagcgcc gcactcgcga gcagatcgac cggctcgccg gcgtgctgag cgactccggc
      901 acccccgctt cgcagcgcgc gaaggtgcac gcggcatgag ggccgccaac gtccccgccg
      961 cggacccgac cgtgaccatc ttcgagcgct cgcgtcccgg gcgccgcgca ttctccgcgc
     1021 ccgagaccga cgtgccggag accccgatcg aggagctcct gcccgcgagc gcgatccgcc
     1081 gcgagccgcc ggctctcccc gagatcgccg agccggagat cgtccgccac tacaacaacc
     1141 tctcgaagaa gaacttcgac ctcgacaccg gcttctaccc gctcgggtcg tgcacgatga
     1201 agtacaaccc gaagctgaac gagcgggtgg ccgcgctgcc gggccacgcc aagctgcacc
     1261 cggcgcagga ccccgagtac gcgcagggtg cgctcgagct gatgtggaac ctcgagcggg
     1321 cgctggccga ggtggccggg ctgccgcacg tgtcgctcca gccgagcgcc gggtcgcacg
     1381 gcgagctggc gggcctcctg ctcacgcgcg cctaccacga ggaccgtggc gagcagcgcc
     1441 acaaggtgct cacgcccgac accgcgcacg gcaccaaccc ggcgaccgtg acgatggcgg
     1501 gctacgaggt ggtgaaggtc ggcaccgcgc cggacggcgg ggtggacctg gacgacctgc
     1561 gcgagaaggc caccgacgac gtggcctgcc tgatgctcac gaacccgaac acgctcgggc
     1621 tgttcgacaa gaacatcgag gagatcgcgg ggatcgtcca cgaggcgggg gcgaccctgt
     1681 actacgacgg cgcgaacctg aacgccgtgg tcggcgtctc gcggccgggc gacatgggct
     1741 tcgacatcgt ccacttcaac ctgcacaaga ccttcacgca gccgcacggc ggcggcgggc
     1801 cgggagcggg gccgatcgcg gtctccgagc ggatccagcc gtacctgccg gtgccgcgcg
     1861 tcgtgcaggt gaagggctcg aatggctccg agccgcactt cgacctcgag cacgaggcca
     1921 cgcagtcgat cggccggctg cgcggcttcc agggcaactt cggcgtgttc gtgcgctctt
     1981 acgcgtacat ccgctcggtc ggcggcgacg gcctgcgcga cgtggccgag accgccgtcc
     2041 tgaacgcgaa ctacctcaag gcgctgctgg ccgagcccgg catcggcgag tacctgccga
     2101 tcgccttcga ccgcacctgc atgcacgagt tcgtcctctc gggcaaaggg gcgaagcagg
     2161 agctcgggat caagacgctc gacatcgcca agcggctgct cgacttccgc gtgcacccgc
     2221 cgaccgtcta cttccccctg ctcgtggacg aggcgctgat ggtcgagccg accgagaccg
     2281 agaccaagga gacactcgac cgcttcgccg aggtcgtgcg cgagctgctc gcggaggcga
     2341 aggaggatcc ggagatcgcc cgcaacgcgc cgtacaccac cccggtgcgc cgcctcgacg
     2401 agggcggggc cgcccggcgc ccggtggtcc gccagcagcc gcctccgcag gacccgcccg
     2461 gcgacccgga cgatcccgag cccgtcagcc gcggctcgct ctagcgggct agcctgccgc
     2521 gcgtgcgcat cttcagcggc atccagccga ccgggcagaa gcacctcggc aactacatag
     2581 gcgcgatccg ccagtacgtc gagggccagg accgcggcga tccggccatc tactgcatcg
     2641 tcgacctgca cgcgatgacc gccccgttcg accccgccga gctgcgctcg aacgtgctcg
     2701 acaccaccgc catcctgctc gcggccgggc tcgagcccga gaagggccgc tgcatcctgt
     2761 tccgccagag cgacgtgcgc gagcacagcg agctctcgtg gctgctcacc tgcctcacgc
     2821 cgtacggcga cctccaacgc atgacgcagt tcaaggagaa gtcggcgtcc gcccgcgagc
     2881 tggtctccgc ggggatcttc ctctacccgg tgctccaggc cgcggacgtg ctgctctacc
     2941 ggccgcacga ggtgcccgtg ggggacgacc agcgccagca catcgagctg ctgcgtgaga
     3001 tcgccgtgcg cttcaactcg cggttcgggg acacgctggt ggtgccggac caccggatcc
     3061 cggacgtggg cggtcgcgtg atggacctcc aggagcccga gcgcaagatg tccacgacgc
     3121 agggcaccga gcagggcacg ctccacatcc gcgacgacga caggacgatg accaagaagg
     3181 tcaagagcgc ggtgaccgac tcgggccgcg aggtggtgcg cgggccgggc aaggccggga
     3241 tcgcgaacct gatcgagatc gaggcggtgc tgcgcggcca gtcgcccgac gacgtcgagc
     3301 ggcgcttcga gggcagcggc tacggcgact tcaagggcgc ggtcgcggag acggtggtct
     3361 ccaccctagg cccgatccgc gagcgctact tggagctggc ggccgatccg gcgcggatcg
     3421 aggcggtgct ggcggacggg gccgcccggg cgcgcgtgct ggcggaggag acgatgaagg
     3481 atgtgcgcga gcggatgggc gtcggaccgg ccggcaccta gggcgcgagc ccgcgcgggc
     3541 gccgatgcca tcgggcgcgg cctctagtct gcccagcgat gcgcgccgcc gccctcgacc
     3601 tcgacctcga ggtcttccag ggaccgttcg acctgctgct cgccctcgtc ctgcgcgagg
     3661 agatcgagct ggccgaggtc gagctggccg aggtcgtcct ctcgtacgtc gagtacctcg
     3721 agcgcaccgg cgagctcgat ctcgaggcca ccaccgagtt cctggtgctg atcgcggcgc
     3781 tgctcgagct gaagtcgcgc ctgatgctgc ccggcgagga ggacgagggc gtcgagtggg
     3841 agcccggcga ggccgccgac gagctgctcg cccgcctgct cgagtactcg cgtcacaagc
     3901 gggcggccga gtacctgcgc gagcgcctcg aggccgagtc gggctttcgc taccgctccg
     3961 ccccgatccc gcccgccctg cgtcgcgtgg cgctcgacct cgccgagaag tcctacgagc
     4021 ccgacgcgct cgccgaggcg atcggcggcc tgctgcgcac gcccccgccg atcgacctgc
     4081 accacatggc gcgcccgcgg gtcaccgtcg agatgcggct ggcccacctg cgcgacctgc
     4141 tgcgctcgcg cacgcgcttc tcgttcgacg acgccgtcga gggcgccgac cgcgtgaccc
     4201 aggccgtcac gatcttcgcc ctgctcgagc tctacaagaa gggcgaggcc gactgggagc
     4261 agagcgcgcc gttcgagccg atcacggtgg tgggccggtg aacgagctgg cccgcgtgat
     4321 cgaggccctc ctgttcctct ccccggagcc gctcacgtcc accgagctcg ccgacgcggc
     4381 ggagtccacg gagggcgagg tggtcgaggc gctcgcgcgc ctgcgcgagc actacgccga
     4441 gggctggcgc ggggtggtcc tgcgtgaggt cggcggcggc ttcacgctgg ccaccgaccc
     4501 ggtcgccgag cgcgccgcac ggcgcctgtt cagccgcccg cgcaccccgc cgctcaccca
     4561 ggcgcagtcc gagtgcctgg cgatcgtcgc gtacctgcag ccggtctcgc gccccgagat
     4621 cgctcgcatc cgcggcgtgg cgtcggagtc ggcggtctcc acgctgctcg agcgcggcct
     4681 gatcgaggag gccggccgct cgcagttcgg cgcgatcctc taccgcagca ccgacctctt
     4741 ccagcgcctg ttcggcctga gcgggctcga cggcctcccg gaccccgccc gcttcgatcc
     4801 gacgccggag gaggagggcg agctgcgcga gcggctgctc aaggccggcg accagcggag
     4861 cagctagcac gccgagcttc gccgagcacg tcgcgatgct cgcgagctgg cgcgagttcc
     4921 cggccgccga gcggaccgcc ggcgagcacg ccgcgctcac cttcgacgac ggccccgacc
     4981 ccgacgcgac ccccgcggtg ctcgacgcgc tcgacgcggc gggcatcagg gcgacgttct
     5041 tcgtggtggg cgagcagctc atgcgccact gggcgatcgc gcgcgaggtg cgggcgcgcg
     5101 ggcacgagct cgccctccac ggcgccgccc acccgcgcca cgccgagctg agcccgcccg
     5161 cggcgcgcga cgaggtggcg cgcgggatcg gcgcgttcga ggccgccgtg ggcgcgcgcc
     5221 cggcgctgtt ccggcccccg tacggccgct tctccgagca ctcctacgcg gcgtgcgcga
     5281 agctcgggct cgagcccgtc tactggtcgg cctggggctc cgactgggag ccgatcccac
     5341 cggcgcggat cgccgacctc gtcgtgcgcg atctcgctcc gggcgcgatc gttctgctcc
     5401 acgactccgc ccgctacgcg gcgcggtcga gcgccgaggc caccgccgcg gccatcgccg
     5461 ccgtggccgc ggccgccgcc gaggctggcg taaggctcgg cccggtgacc ggctccccgt
     5521 gaccgatacc cccccgtaag gggggagcaa ctccaccccc ggctggggga tacccgcgca
     5581 gcctcacgaa ttggcgcgtg agcgccgata cgccgtgtag acgccgatgc aagccgccgt
     5641 tccggagaag ccccgcaggt tcctacgggc cgttcaggtc ctgtcgttcc ttgctctggc
     5701 cggatacgcc ctgcacgtcg gcgtcggtct cggtggggac gggatggagc cgttcttctc
     5761 ggactacgtc tacaacggcc tggtgctcgt ggcgacggcg tcgtgcctcg tgcgggccgt
     5821 tcgggtacgc gcgcgccgcg cggcctgggc gtgcctgggc gcgggcctcc tgagctgggc
     5881 gttcgccgac ctctacaaca ccttctacct cgcgaagctc gaggagccgc cgtatccgtc
     5941 gctcagcgac gcgttctggc tcgcgttcta cccgatgacc tacgtggccc tcgtgctgct
     6001 cgtgcgcgag cgcatgaagg aggcgcgcgc gagtctgtgg ctcgacggca tcgtggcggc
     6061 gctcgccgtc tgcgccgtcg gcgaggtgct cgtcttcagc ccgatggcgc aggccagcct
     6121 cgagggcggg ctctcgaccc cgatcgaggt cgcgaccgac ctcgcctatc cgatcggcga
     6181 catgctgatg ctctcgctcg tggtcgtggt gttctcgctc acggcctggc ggccggggcg
     6241 cgcctggacg atgatcggcc tcggcctggc cgccatggcg gccggcgacg gcgtctacct
     6301 ctaccaggcg tcgcagggca cctacgtcga ggggacgatc ctcgacgcca tgtggcctgc
     6361 cgcgaccctg ctcgtcgggt tcgccgcctg ggagccgtct cgccgcaccg gcgacgagat
     6421 cgatctcagc ggctggcgga tgctcgtgct gccggtcctg ttcgcgctgc cgtcgctggg
     6481 cgtgctcgtc tacgaccact ggaccgaggt cgacggcgcc gccgtcctgc tcgccaccgt
     6541 ctgcatgctc gccgtgatcg tgcgcacggc catgacgttc ggcgagaaca tgaggatgct
     6601 caccacgagc cgtcgcgagg cccacacgga cgctctcacc ggcctcggca accgccgccg
     6661 cctgatggcc gacctcgacc gcgagatgga gctggccggg ccgcactccc cgcgcgccct
     6721 gggcctcttc gacctcgacg gcttcaagcg ctacaacgac aacttcggcc accccgccgg
     6781 cgacgccctg ctcgcgcggc tcggccgcgc cctgcgcgag gccgtagagc cggaggcgcg
     6841 cgcctaccgc ctcggcggcg acgagttctg cgtgctcggg gcagccgacg ccaacgcggc
     6901 cgccgccctc gtggcaaccg ccaccgccgc actctccgag cagggtcagg gtttcttcgt
     6961 caccgcttcg cacgggctcg tgctgctgcc gctggaggcc caggacgcga gcgccgcgat
     7021 gcagatcgcc gaccagcgcc tgtacgggca gaagggctcg cggcgcagca gcgcggtggg
     7081 ccagcagacc cgcgacgtgc tgctcgaggt cctgcacgcc cgcaagcccg acctgcacgt
     7141 gcacctccac gaggtcgcgg aggtcgccct cgtggtcggc caccgcatga agctctcggc
     7201 cgaggagctc gacgagatgg cccgggcggc cgagctgcac gacatcggca agatggccgt
     7261 gcccgacgag atcctcaaca agccgggccc gctcgacgac cgcgagaccg agttcgtgcg
     7321 ccagcacacg ctcgtcggcg agcgcatcct cgccgcggct cccgccctcg aggaggtggc
     7381 caagatcgtc cggtcgagcc acgagagcta cgacggcagc ggctaccccg acggcctggt
     7441 gggcgaacgg atcccgctgg cggcccggat catcgccgtc tgcgacgcct tccacgcgat
     7501 gaccgacaag cgcccgtacc agccggcgat gacgccggct gaggcgatgg ccgagctgcg
     7561 ccgctgcaag gggacccggt tcgacccggc cgtggtcgag gcgctgtgcg ccgagatcgg
     7621 cgccggcggg atcccgccgc gcgtgcgccg cctgccggac gccgctcccg ctcccgctcc
     7681 cgccgcaccg cccgtcgagg ctcggtgacc cgcttccgcg gggtcgcggt ggccgccgcg
     7741 gccgccgcgc tggcgctgcc cgcaggcgca tccgccgcga ccgtgagctc cgccggcgga
     7801 gccgtcacgt tcaccgctgc gtcgggtgag tcgaaccgcc tttcggtctt cgaggccggc
     7861 gggacgctgc tcttcgagga cgcgggcgtc agctcgatga ccgcggggcc ggggtgcact
     7921 ccggcgggcg cgcagcgggt cgtgtgcgcg gtgcacggcg acggtgcggt ggcggtccag
     7981 ctcggcgacg gcaacgacgt ggtcgtgagc caggtcccgc tgcccttcgg cgtccacggc
     8041 ggcctgggcg acgacgggat cgtgctctcc gcgggcaacg acgcggtgga cggcggcgcg
     8101 ggcgccgaca cgatcgacgc cggcggcggc gacgacgccg tggacggcgg cgcgggcgcc
     8161 gacacgctcg acgccggcgg cggcgacgac tcgctcgcgg gtggcgccgg ggtcgactcc
     8221 ctcgccggcg gcgcgggcgc cgacaccctc cgggcgcgcg acggcgccgc cgagccgctc
     8281 gcctgcggct ccgagaccga cgcggtcgag gccgaccacg gcgacgccgt ggactccgat
     8341 tgcgaggtcg tcgaccgcgg cgagaccgcg cagccgacct tcgcgccggt gctcccaccg
     8401 gacccgaccg gtggcgtggg cagcgcgatc gaggccccgg tggccgcgat ctccggcgcg
     8461 tcgctgcacg tgactcgagc gggcctgacc caggtccggc tcaggtgccc gcgcacggcg
     8521 ttcgagggct gctcggggtc gatcgtggtc gagccgctcg gcgtggccgc accgaactcg
     8581 cggctcgacg tgaccggcgc gcggcgggta cccatccggc tcggcaccgc ccgcttcagg
     8641 gccctcgcgg gcgccggcat cacggtcccc gtccggctcg agccccgcgt ctggcgccgc
     8701 ctcgccgcgc ggccgcgcgc gaaggtgcgg atcaccgtga caatggcgaa cgccacgggc
     8761 gcgacgacca gcgtccggac cgtccgggtc cggcgcgcgg gctaggcggc gctggccgcc
     8821 cggcgcgcgg gctaggcggc ggtggccgcg agctgcccgc aggccgcgtc gatgtcgcgg
     8881 ccgcgagtta ggcgcacggt cgcgcgcagg ccgcgctcct cgagcgtcgc gcggaaggcg
     8941 tcgatcgccc cgcgcgtcga gccgcgcagg tcggagtccg tcgggttgta cgggatcagg
     9001 ttgaccttga atgcctcgcg cggcttcagc acgtcggcga gcgccatcgc ctgctcgtag
     9061 cggtcgttga ccccgtcgag catcaggtac tcgacgaaca ccggctgtct gcgcgtgcgc
     9121 gaccagcgca ggcacgcgtc gagcacgtcc ggcagcgggt agcggtcgtt caccggcatc
     9181 agctcggagc gcagcgcctc gtccgccgcg tggagcgaga gcgccagccg cacggccggg
     9241 ccgtcctcgg tgagcagctc gatccccggg atccagccga cggtggagat cgcggtgcgc
     9301 cgcgaggcga tcccgaggtc gggcaggcgc tcgcaggcgg ccagcacgtt gtcgaggttc
     9361 atgagcggct cacccatgcc catgaagacc gcatggtcga ccggctcgat ccttctgaag
     9421 tgcagcgctt gatcgaggat ctccgagctc gtgaggttgc gcgcgaagcg catccggccg
     9481 gtcgcgcaga acgtgcacgt gagcgggcag cccgactggc tcgacaggca gagcgaccgg
     9541 cgcccgtcgc ggtagcgcat cagcaccgcc tcgatcggcc gcccgtcgga ggtgttgaag
     9601 agggccttct cggtgccgtc gcgggagcgc gcctcgcgtg ccagctcgag gctcgacagc
     9661 ggcacgtccg ccgcgagtcg ctcgcgcagg gccgccggca ggttcgtcat ctcctcgtag
     9721 gaggaggcgc cgcgcgccgt ccactcccag atctgcttgg agcggaaggc gggctcgccc
     9781 gcgcgcgcga gcacgtcgtc gaggaggttc aggtccatgc gccgagtgtg gcagcctcac
     9841 ccggatgcgc ctcgcgaagt tcctagccca ctccggggtc gcctcgcggc gcgccgcgga
     9901 gcggctgatc gcctccgggc gcgtgagcgt gggccgcgag gtggtcaccg acccggcgcg
     9961 cgacgtcgac gccgattcgg gcgtggcggt cgacggcgag ctggtccgcg tcgagcgccg
    10021 cgtcgtctac gcggtgaaca agccgcgggg ggtcgtctcg acggcggccg acacccacgg
    10081 gcgcccgacg gtggtcgagc tgctcccggc cgaggcgcgc cggctctacc cggtcggccg
    10141 cctcgacgcc gactcgaccg ggctcctgct gatgacgaac gacggcgagc tggccaacct
    10201 cctcacgcac ccgcgctacg gggtggagaa ggtctaccga gcgcacgtga agccaccccg
    10261 catcaccgaa cgcgccctca aggccctgcg cgagggcgtg gagctcgacg acggcatggc
    10321 ccgcgccgcc cgcgcccgct cggtgtccgg aggcgtgctc gaaatatcac tgagggaggg
    10381 gaggaagcgc caggtcaggc gcatgtgcga ggccgtgggc ctcacggtgg tcgagctcga
    10441 gcgcgtgcgt ttcgggccgt tacgcctggg cggcctgagg caaggctctt tccggcggct
    10501 cacggggccg gaggtggagc aactcaggga agcggcgaac ggggggtgac tgcgggggcg
    10561 ggtgcggttg cgtcgctctg aggggggctg ctgagggagc ctgcgaccgt acccccgaag
    10621 agcgatgcaa acgcacccgc agcaggcggc aagagccatg cttgaatcag caccaccatg
    10681 aggctgagag cactcagagg ggcgatcacc gtcgacggca acgagcgaga cgcgatcctc
    10741 gagggcaccg aggagctgct gcgcgaggtc atggggcgca acgagctggc cgccgaggac
    10801 atcgtgagct gcgtcttcac ctgcacagcg gacctgaacg ccgccttccc ggccgaggcc
    10861 gcgcgccggc tcgggctggg cgcggtgccg ctgctgtgcg cgcgcgagat cgatgtgccc
    10921 ggcgcgctgc cgcgcgtgat ccggctgatg gcgcactgct acgcggagga ggaccgccag
    10981 gcgcagcacg tgtacctgcg cgaggcggtc gagctgaggc gcgacctgga gggtgcgcag
    11041 tgagcctcga gttcaccgcg cgcctgcgcg acatcccgac ctatcccgcc gcggcgacct
    11101 acgagtacgg cggcgagctg gtcaagctcg cgtcgaacga gacgccgtac gccccgcacg
    11161 ctgcggtggt cgaggcgatc caggcccagc tcggggcgct caaccgctac ccggaccccg
    11221 agaaggggct gctgcgcagg cgcatcgccg agcgattcga gacgaacccc ggccgagtag
    11281 cggtcggcaa cggctcctgc gagatcctga tggccgcggg cgaggcgctg ctcgagccgg
    11341 gcgccgagat cgtctacgcg tggccctcgt tctcgatgta cccgcacctg gcggcggcga
    11401 gcggcgcgcg cgcgatcgag gtcccgctga ccgacggcgg ccaccacgac ctcgacgcca
    11461 tggcgcgcga ggtgaccgcc gcgacgcgcg tggtgctcgt ctgcaacccg aacaacccga
    11521 ccgccacggc gctgccggtg gaggagatcg acgcgttctt ggcctcgctc ccgcgccaca
    11581 ccgcggtgat cctcgacgag gcctacgtcg agttctcgac aatgcaggac ccggacgagt
    11641 cgctgccgct gctggcgaag cacccgaacc tggtgctgct gcgcaccttc tcgaaggtct
    11701 acggcctctg cggcctgcgg gtgggctacg gcctgtgcgg ctcggaggac ttcaggatcg
    11761 cggtggacaa ggtccgccag ccgttctcgg tgaacgcgct cgcccaggcg gcggcgatcg
    11821 aggcgctgcg ccaccaggac gaggtcgagc gccgtgtgga gcgcacggcg atcgagcgcc
    11881 tgcacgtcga ggacgagctc gaggagcgcg gcctctggtc gagcgacagc caggccaact
    11941 tctcctggtt cgccctcggc gaccgcgacg aggcggcggt gctgaagggc ctggccgagc
    12001 gcggcgtgat cgtgcgcggc ggcaccgacc tgggccacgc cggccacatc cgcgtgacct
    12061 acggcacgcg ctcggagaac gatcgcttcc tggccgcgct ggacgaggcg ctggccgcgg
    12121 cgccgaagtc gagctaggtc cgcagcagga tcgtcacgaa cagcccgagc agggccgtga
    12181 actggacgat cgcgaagttg aacgcctggc ggtagaggtc gtcgatcctt gcttctaggc
    12241 gatcgagccg cgcctcgacc cgatcgagcc gcgcctcgac ctgctcgaag cgcgcttcga
    12301 tccgcgcgac cgtgtcgtcg atcctctcgt cagtccacaa ggtgcgctcc atgcattact
    12361 agagcgtacc cccggaaaag ttcgcccccc gggggatctt cacccctcaa ggcgtggatg
    12421 ccggaaccga tacgtggtag aacagagtcc cgcgatgaac cgcaccggca cgtcacccag
    12481 cagcacctgc gcctccggcg ccgcctggcg tgcctatctc tggacctggc gatttagcta
    12541 ggcgtccggc cgcgcgcgca cccagggcga gcgcgagacc gagtcagagc tccccgccgg
    12601 acgccgctct gcccacggcc caaaccacgg gtcgcgtcgt accagaacta ggatttcgtc
    12661 cacgagcttc gaaagcaggt cctgatgatg atcgtcatga aagagggtgc cgggcccgac
    12721 gaggtggacg cggtcgtccg ccaggtcgag tccaccggcg ccaccgcaca cgtctcgcgc
    12781 ggcgagatac acaccgtcgt gggcgcgatc ggcgaccgcg agcacatagc caacctcgag
    12841 ctcgacggct tccccggggt cgaccacgtg gtcccgatca gccgcccgta caagctcgcc
    12901 tcgctccagt tccgcaagga gcagccgtcg gtgctcgaca tcggaggccg caagatcggc
    12961 gggccgcact tcatgacgat cgccggtccg tgcacggtcg aggaccgcga gaccatgctc
    13021 gaggcggcgc gcatcgtccg cgactccggc gcgcagctcc tgcgcggcgg cgcctacaag
    13081 ccgcgcacct cgccgtactc gttcagcggg ctcggggtgg aggggctgcg gctcctccag
    13141 gaggccaagg acgagaccgg cctgccgatc actaccgagc tcatggacgt gcgcgacctc
    13201 gaggacgtgc tcgaggtggc cgacgtgatc cagctcggcg cccgcaacat gcagaactac
    13261 acgctgctca ccgaggtcgg ccgcgccggc cggccggtgc tgctcaagcg cgggctcgcg
    13321 aacaccctcg aggagttcct catgtcggct gagtacatcc tcaaggaggg caacgagaac
    13381 gtgatcctct gcgagcgcgg cgagcggacg ttcgagccga actaccgctt ccagctcgac
    13441 ctgatggcga tcccggtgct gaaggagatg acgcacctgc cgatcgtcgt cgacccgagc
    13501 cacgcggcgg gcaagcgctc gctggtcccg gcgctctcct acgcggcggc cgccgcgggg
    13561 gccgacggga tcatcgtcga gatccacccg gagccggagg cggcggtttg cgacgggccg
    13621 caggcgctct acgccgagga atttcccgag tacctgcgca agctcgagca ggcggcggcg
    13681 ctcgcgggca aggagacagt gcgggtctga gccgcgtcgc gatcctcggg gtgggcctga
    13741 tcggcggctc gatcgggctc gccgcgcgcg agcagggcag cgaggtggtg ggctgggatc
    13801 ccgatcccgg cacgctcgac cgcgcgctcg agctgggtgc catcggggcc gcggcc
//
LOCUS       DASVPL010000131        14015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_16432, whole genome shotgun sequence.
ACCESSION   DASVPL010000131 DASVPL010000000
VERSION     DASVPL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14015
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16432"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /gene="pheT"
                     /locus_tag="VF517_06725"
     CDS             complement(<1..1243)
                     /gene="pheT"
                     /locus_tag="VF517_06725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX8052668.1"
                     /translation="MRVPVAWLRSYCDPALGAGEIADALTMAGIKLERLHRTGVGDPG
                     AFVVGRVLEASKHPDADRLSVCTVDDGSGEARTIVCGAPNVATGQTVAVALPGAVMPD
                     GTKLGEAKLRGVRSSGMILAEDEVGIGEDHAGIMVLDESLAPGAPLAEVLPIADEAIE
                     IEITPNRPDAMSVYGVARDLHAVTAAPLAEDPTAADAEPSGSDSASDHASVTIEDPDI
                     CLRFTARVFEDVRIGPSPLWLKQRLMAAGQRPISNVVDITNYVMLACGQPVHAFDLDR
                     VRGGRIVVRRAREGETMTTLDGVERTFSPEAALVCDAEGPSGIAGIMGGQISEVSDPD
                     AERPTTRVLMEAATWVGPNIMRTSKALGLRTEASSRFEKQLHPELAMAAQRLAAQLMV
                     ELCDARLVPGTIDVYPRPAEPR"
     gene            complement(1240..2304)
                     /gene="pheS"
                     /locus_tag="VF517_06730"
     CDS             complement(1240..2304)
                     /gene="pheS"
                     /locus_tag="VF517_06730"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX8052669.1"
                     /translation="MADAPARFDELRREGEAAIAAAGTSAELEELRVRYLGRRSELTQ
                     ALRSIGELPPEQRGPVGKGANQVKQALEGLLAERTARIDAEELDRRLAEDRIDVTLPG
                     DPPLPSGRLHLVSQIRRELEDVFVGLGFAVLEGPEIEYEYYNFTALNHPPEHPARLPQ
                     DTFYLSESVLLRTHTSPMQVRAMEAQEPPIYIVVPGRVYRPDTPDATHVPMFHQLEGL
                     AIDEDITLADLQGVLLEFARAMFGADMKVRLRPGYFPFTEPSVEVDVSCFVCGGTGFT
                     GSQRCHTCRGEAWIEILGAGMVDPNVLGYVAESGYDPERVQGFAFGMGIERIAMIKHG
                     VPDLRMLYENDVRLLEQFGA"
     gene            complement(2297..3010)
                     /locus_tag="VF517_06735"
     CDS             complement(2297..3010)
                     /locus_tag="VF517_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX8052670.1"
                     /translation="MITSPDNNQLKTIRKLQDKRHRDRERLFVAEGEDLVAAARDAGW
                     EPEMLLVSGEDVEPKLLDAVSALGSGTRVIGVYPQRWAEPGGELAVYLHGVRDPGNVG
                     TVMRSAHALADGPVVLGPGCADPWSPKAVRASMGSVFARPPARAEPEAARAVFAERGG
                     ATIALQARTGAPLDEVPSGSPELLCLGGERDGLPPAVVEAADAVAHLPVRGDGPDSLN
                     VAMAATVALYERNRMARHG"
     gene            complement(3075..3431)
                     /gene="rplT"
                     /locus_tag="VF517_06740"
     CDS             complement(3075..3431)
                     /gene="rplT"
                     /locus_tag="VF517_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX8052671.1"
                     /translation="MRVKRSTHARKKRRATLERTKGYRGEAHSSYKRAKEALLKADTY
                     AYRDRRNRKRDFRRLWIQRINAAARREGLTYSQMMHGLKLAEIELDRKVLADIAVRDP
                     ETFRRFAERAREAAAT"
     gene            complement(3428..3625)
                     /gene="rpmI"
                     /locus_tag="VF517_06745"
     CDS             complement(3428..3625)
                     /gene="rpmI"
                     /locus_tag="VF517_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX8052672.1"
                     /translation="MPKMKTHSGAKKRFKVTAKGKVRGRHAMRSHILGKKNAKRRRRL
                     DEPAIMSDADTPKIKALLIKK"
     gene            3704..4558
                     /locus_tag="VF517_06750"
     CDS             3704..4558
                     /locus_tag="VF517_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052673.1"
                     /translation="MDMTQTQLSQRPLAALAATQSKEDKLAHALDFELLVEDRLATEH
                     TPYRCGTALFRRDLPRVAALNVLRVESGVEALDAAALIEFADGLQTGLPQRSLRVVDD
                     QLAAQLRPVFAAAGWITAGMTLMAPRRRPDRPIDTTSVHAVELETIAYARQATLGRAY
                     RDLDTAEQLLVAGEMPALGTEVRAFASTVGNEVAAYAVARVAGHVAKLTEVDALVRSH
                     GHGLGRALIWGAVKELRAEGVRLVVVEAQDDAWTRWIYLRLGFEDIGRTHRFVRAWGD
                     EPAAPPAI"
     gene            complement(4586..5089)
                     /locus_tag="VF517_06755"
     CDS             complement(4586..5089)
                     /locus_tag="VF517_06755"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX8052674.1"
                     /translation="MGQDPDAIRQDIEQTREQMGETIGAIGYKTDVKERAKDSVSEKT
                     DAIKSKVGGVMGRANDATPSTGEMKQGAQQAVSVAQENPLGLAIGAVAAGFIAGMLIP
                     STRVEDEKMGEMSDQVMNQVKETASEAVDRGKQVAQEVAQTAQETAKESGQQQAQELA
                     SSASPSS"
     gene            complement(5053..5475)
                     /locus_tag="VF517_06760"
     CDS             complement(5053..5475)
                     /locus_tag="VF517_06760"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX8052675.1"
                     /translation="MATNGSSDDLRERPIGELLKQLSEETTTLVRQELELAKAEMAEK
                     GKKAGKGAGMFGGAGVVGFLALGALTAALILALDTGMKAWLAALIVGLVYAAIAGVLA
                     LTGKKEVQQATPPVPEQTVESVKEDVQWAKTQTPSART"
     gene            complement(5484..6134)
                     /locus_tag="VF517_06765"
     CDS             complement(5484..6134)
                     /locus_tag="VF517_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052676.1"
                     /translation="MAEVSTPPQGATAPSSQDEQSGAAAQAQETASQAKDAAQDKAQE
                     VMGQAQEKAQEAKQQAGSKVREQVDQRSKQAGEQVSTTASDLRTVGETLREQGKETPA
                     KLAEQAAERTEKVGSYLTESDADKILSDVEDFARKQPWAVVAGGLLLGFAASRFMKAS
                     SSQRYSQRSQTGSGASGQLQSGSTGSNGYSSGTTPEVYAPPPTGAPVAPGVTPGQV"
     gene            complement(6272..7141)
                     /gene="infC"
                     /locus_tag="VF517_06770"
     CDS             complement(6272..7141)
                     /gene="infC"
                     /locus_tag="VF517_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX8052677.1"
                     /translation="MPIPRRFDRRPPERDQTRINERIRVPQVRLIGDDGNQVGVVPTD
                     QALRYAQERDLDLVEVAAEARPPVVRVLDYSKYKYEQEQKAKAARRHQKQVTVREIKL
                     RPKIANNDYETKKGHVVRFLKHNDRVKVTIMFRGRETTHPERGEALLMRLAEDIGELG
                     VIEQRPNLDGRNMTMVMAPARVTEEGEHEEHVEEPGTDHERPAKRERREVRLRREAAE
                     ADGGDEGDGDEAAAPAATGAAPAPDDASPAGDDAEAAAPATAADADAPAAADEDASAA
                     SDDAPAGGDSAAA"
     gene            complement(7331..9247)
                     /gene="thrS"
                     /locus_tag="VF517_06775"
     CDS             complement(7331..9247)
                     /gene="thrS"
                     /locus_tag="VF517_06775"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX8052678.1"
                     /translation="MQVTLPDGTPLELEDGATGADAARAIGEGLARAALAIRQNGALK
                     DLSAPLEDGKPIEIVTPKSPDALELIRHDTAHVLATAVIDLYPGTKVSIGPPIEDGFY
                     YDFEFPEGVKVGEDDLPKIEEAMRRHVKADESFERSDVSTAEALDRFTREEQPYKVEL
                     IQDLIQNEAVETVSLYRNGPFTDLCRGPHGPTTKRIKAFKLTSVAGAYWRGDENRQML
                     TRIYGTAFLSKEDLDEHLEMLEQARARDHRKLGRELGLFMLSDLSPGSPFWLPAGTHV
                     WNELTKLWRTENVGRGYVEVRTPILYGVELWKQSGHWHVYRDNMYFTDVEERPMGLKP
                     MNCPAHVQIYKRDLRSYRDLPIRYAEQGLVHRHEPSGTLHGLLRVRHITQDDAHVFCT
                     EDQIEEEVLRCLDFGFFIYDHFGFEPRLELSTRPEKRVGTEAMWDRAEAALARALESR
                     GLDYDLNEGDGAFYGPKIDLHMTDSLKRSWQLGTVQLDYYMPEQFELTYTGADNAEHR
                     PVMIHRALLGSFERFIGILIEHYAGELPLWLTPVHALVLPIADRHNDYAREVEERLRG
                     AGIRVEVDDRTESVGRKIRDGELRKVPLMLVVGDKEQEAGQVSVRRHREGDQGAEPVD
                     AVIERLRDEISARS"
     gene            9550..10044
                     /locus_tag="VF517_06780"
     CDS             9550..10044
                     /locus_tag="VF517_06780"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX8052679.1"
                     /translation="MRKLLLSLACVAAAVPAVALAADEDDVQVSGTTPQADERPADAR
                     QKAKRGQARIERTHERAEKRRQRARARKRARARARVNAGGSGGGATPAHLQAIAACES
                     GGNPSAVGGGGAFRGKYQFDRGTWASVGGSGDPAAAPEAEQDRRAAILYSRAGSSPWP
                     VCGS"
     gene            10149..10751
                     /locus_tag="VF517_06785"
     CDS             10149..10751
                     /locus_tag="VF517_06785"
                     /inference="COORDINATES: protein motif:HMM:NF040123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6518 family protein"
                     /protein_id="HEX8052680.1"
                     /translation="MRLVALTLLAGVLVGTIGWASDGGPLLVRWTSAIAAGWLIGAFV
                     VGALAGSGLRAAAAGAGTIVAGVATYYVLFHFVSGELSLRYGVVVGVAWSSVGIGIGA
                     VLGWAGDAWRRRHADSIGVALLSGALAGEAMLLLGEWQNRAARVVLICELVLGAALPY
                     LLARPKLARTVALSAAVAVAVIGVESAVRGTMRLAGWAGA"
     gene            complement(10748..11503)
                     /locus_tag="VF517_06790"
     CDS             complement(10748..11503)
                     /locus_tag="VF517_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8052681.1"
                     /translation="MIVERSMSPDFLSNAYLVADEPGGHGVIVDGGADPEPLLAAVEQ
                     NELTIPYLLLTHHHHDHVSENHVFQERLGVKILAHPLEAARLMDVDRAIEPGEVLEVG
                     GLRIDGLHTPGHTDGMLSFRVNDAEIYTGDTLFKGSVGGVRAPGSTTFEDLKASIMDV
                     LMKLPPETVVRPGHTEPTTIGDEWEQNAFVRVWRGVDEESDERCTVWDEEATLVLWAP
                     DYDGGHKAWVRWTDSGRDDIVPGSQVVRVDPPG"
     gene            11530..12606
                     /locus_tag="VF517_06795"
     CDS             11530..12606
                     /locus_tag="VF517_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8052682.1"
                     /translation="MDAAALRAEFPVLAQLAYLNAGTQGPVPARAAAAATATVEHERD
                     AGRGDTAHFEALFALGERMRAHVARLFACDADEVALTHSTTDGMNAVLGALDLGPGDE
                     VLTSDEEHPGLLGPLATNRDRRGFDVRVAPFAEIANAIGPRTKLVACSHVSWVNGKVV
                     DTAALAAADPLVLFDGAQGLGALPTPMRELGCDFYGAAGQKWLCGPDGNGYLYVRGDL
                     SADLPAPWSSYTTFADPSDPLGSGLKPGARRFDLGFSAPHWSWSVAALDLLADAGWDE
                     LLARGPALAARLAGELTERGVQVEPRGPSTLVSWRSEDEAAETRRLADAGVRVRDIPG
                     HGLVRASVGAWSSEEDLERLLAAR"
     gene            complement(12603..13277)
                     /locus_tag="VF517_06800"
     CDS             complement(12603..13277)
                     /locus_tag="VF517_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8052683.1"
                     /translation="MSPSEAPLRLAHELPRGRHNLPREVVLRSQRERMIDAVIERVAA
                     KGYAATTVADVIGQAAVSRKTFYEHFSDKEECFIAAYDEITRQLMGSVVEAYEGRPAW
                     AERVASALGILLERLASRPALARFCIIEILAAGPRALERRDAVLQHFTELLAPGRHEA
                     PAGLTIPELTAQAVVGALYSLIYDEIFHGRVRELPRMTPDLVYIALAPYVGPRAAVRA
                     AQSAGC"
     gene            13500..>14015
                     /locus_tag="VF517_06805"
     CDS             13500..>14015
                     /locus_tag="VF517_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052684.1"
                     /translation="MKVRTKAAAAICGAIATVAITAMAAWAAGSTTITPAGDYTKASL
                     KSGTNAQFTIAGFLSVTCNQSTSAPTSPLGTNTKNQIPPAPGNHNASGAVTLPVNAPS
                     FTNNGGACPTSLSGVTATTTTSGSWTLSGQNGSPITGSLTIPKAGAVTSTSNGCTITV
                     APSGPVSVGGTW"
BASE COUNT         1801 a         5083 c         5002 g         2129 t
ORIGIN      
        1 gccgcggctc ggccggccgc gggtagacgt cgatcgtgcc cggcaccagc cgcgcgtcgc
       61 acagctcgac catgagctgc gcggcgagcc gctgggcggc catcgccagc tccggatgga
      121 gctgcttctc gaaccgcgac gaggcctcgg tgcgcagacc gagcgccttc gaggtccgca
      181 tgatgttcgg ccccacccac gtggcggcct ccatcagcac gcgcgtggtc gggcgctcgg
      241 cgtcggggtc ggacacctcg gagatctgcc cgcccatgat ccccgcgatc ccgctcggcc
      301 cctcggcgtc gcacacgagc gcggcctcgg gcgagaacgt ccgctccacc ccgtccaggg
      361 tcgtcatcgt ctcgccctcg cgcgcgcggc gcaccacgat ccgccccccg cgcacgcggt
      421 cgaggtcgaa cgcgtgcacg ggctggccac acgcgagcat cacgtagttg gtgatgtcca
      481 ccacgttcga gatcgggcgc tgccccgccg ccatcaggcg ctgcttgagc cagagcggcg
      541 acggcccgat ccgcacgtcc tcgaacacgc gcgcggtgaa gcgcaggcag atgtcgggat
      601 cctcgatcgt caccgaggcg tggtcggagg ccgagtccga ccccgacggc tccgcgtcgg
      661 cggcggtcgg gtcctcggcc agcggggcgg ccgtgaccgc gtgcaggtca cgcgcgacgc
      721 cgtagacgga catcgcgtcg gggcggttgg gcgtgatttc gatctcgatc gcctcgtcgg
      781 caatcgggag cacctcggcc agcggcgcgc cgggagccag cgactcgtcg agcaccatga
      841 tcccggcgtg gtcctcgccg atcccgacct cgtcctcggc gaggatcatc ccgctggagc
      901 gcacgccgcg cagcttcgcc tcgcccagct tcgtcccgtc gggcatcacc gcgccgggca
      961 gcgcgacggc cacggtctgg ccggtggcca cgttcggcgc gccgcagacg atcgtgcgcg
     1021 cctcgcccga cccgtcgtcc accgtgcaca cgctcaggcg gtcggcgtcc gggtgcttcg
     1081 acgcctcgag cacccgcccc accacgaacg cgccggggtc gcccacgccg gtgcggtgca
     1141 ggcgctcgag cttgatcccc gccatcgtta gcgcgtcggc gatctccccg gcgccgagcg
     1201 cggggtcgca gtacgagcgc agccatgcga ccgggaccct catgcgccga attgctccag
     1261 caggcgcacg tcgttctcgt agagcatgcg caggtcgggc acgccgtgct tgatcatcgc
     1321 gatccgctct atgcccatcc cgaaggcgaa gccctgcacg cgctcggggt cgtagcccga
     1381 ctcggccacg tagccgagca cgttcgggtc gaccatcccg gcgccgagga tctcgatcca
     1441 cgcctcgccg cggcaggtgt ggcagcgctg cgagccggtg aagccggtgc cgccgcagac
     1501 gaagcaggac acgtcgacct cgacgctcgg ctcggtgaac gggaagtagc ccgggcgcag
     1561 ccgcaccttc atgtcggcgc cgaacatcgc gcgggcgaac tcgagcagga cgccctggag
     1621 gtcggcgagc gtgatgtcct cgtcgatcgc cagcccctcg agctggtgaa acatcggcac
     1681 gtgcgtggca tcgggggtgt ccggccggta cacgcgcccg ggcacgacga tgtagatcgg
     1741 cggctcctgc gcctccatcg cgcgcacctg catcggcgag gtgtgggtgc gcagcagcac
     1801 cgactcgctg aggtagaagg tgtcctgcgg cagccgcgcc gggtgctccg gcgggtggtt
     1861 gagcgccgtg aagttgtagt actcgtactc gatctccggc ccctcgagga cggcgaaccc
     1921 gaggccgacg aagacgtcct cgagctcgcg ccggatctgc gagacgaggt gcagccggcc
     1981 ggacggcagc ggcgggtcgc ccggcagcgt cacgtcgatg cggtcctcgg cgaggcggcg
     2041 gtcgagctcc tcggcgtcga tgcgcgccgt gcgctcagcc agcaggccct cgagcgcctg
     2101 cttcacctgg ttcgcgccct tgccgaccgg accgcgctgc tcgggcggca gctcgccgat
     2161 cgagcgcagc gcctgggtca gctcggacct gcggccgagg tagcgcacgc gcagctcctc
     2221 gagctccgcg ctcgtgccgg cggcggcgat ggcggcctcc ccctcgcgcc tcagctcgtc
     2281 gaatcgggcg ggcgcgtcag ccatggcggg ccatcctatt ccgctcatac agcgccaccg
     2341 tggcggccat cgccacgttc agcgagtcgg gcccgtcgcc acgcaccggc aggtgcgcga
     2401 ccgcgtccgc ggcctcgacc acggcaggcg ggagcccgtc gcgctcgccg ccgaggcaga
     2461 gcagctcggg gctcccgctc gggacctcgt cgagcggcgc gccggtgcgg gcctggagcg
     2521 cgatggtcgc gccgccgcgc tcggcaaaga cggcgcgcgc ggcctccggc tcggcgcgcg
     2581 cgggcgggcg cgcgaacacg ctgcccatgc tcgcgcgcac ggccttcggc gaccacgggt
     2641 ccgcgcagcc gggccccagc acgaccggcc cgtcggccag cgcgtgcgcc gagcgcatca
     2701 ccgtccccac gttgccgggg tcgcgcacgc cgtgcaggta cacggccagc tcgccgcccg
     2761 gctcggccca gcgctgcggg tacacgccga tcacgcgcgt gcccgagccg agcgcgctca
     2821 ccgcgtcgag cagcttcggc tccacgtcct cgcccgacac gagcagcatc tcgggctccc
     2881 agccggcgtc gcgcgcggcc gcgaccagat cctcgccctc ggccacgaac aggcgctcgc
     2941 ggtcgcggtg gcgcttgtcc tggagcttgc ggatcgtctt cagctggttg ttgtctggtg
     3001 aggtgatcat cgggccacta aaaagggcgc ctcgcggggc gccctgggag tgagtccggt
     3061 cggttcctga aggatcaggt agccgccgcc tcccgggcgc gctcggcaaa ccgtcggaac
     3121 gtctcggggt cgcgcacggc gatgtcggcg agcaccttgc ggtcgagctc gatctccgcc
     3181 agcttcaggc cgtgcatcat ctggctgtag gtcaggccct cgcgacgagc cgccgcgttg
     3241 atgcgctgga tccacaggcg ccggaagtcg cgcttgcggt tgcggcggtc gcggtacgcg
     3301 taggtgtccg ccttgagcag ggcctccttg gcccgcttgt agctggagtg cgcttcgccg
     3361 cggtagccct tcgtccgctc gagcgtggcg cggcgcttct tgcgcgcgtg ggtggagcgc
     3421 ttgaccctca cttcttgatc agcagtgcct tgatcttggg cgtgtcggcg tcggacatga
     3481 tcgccggctc gtcgagacgg cggcggcgct tcgcgttctt cttgccgagg atgtggctgc
     3541 gcattgcatg acgtccccgg accttgcctt tggccgtcac cttgaagcgc ttcttcgccc
     3601 cggaatgggt cttcatcttc ggcatgagtc cgccgactat agcccgcggg cggtgcgatc
     3661 ttcgtccaga cggcgaggtc gcggccccgg aacggtcgta ctcgtggaca tgacccagac
     3721 ccagctctcg cagcgcccgc tcgccgcgct cgctgccacc cagtcgaagg aggacaagct
     3781 cgcccacgcg ctcgatttcg agctgctcgt ggaggaccgg ctcgcaaccg agcacacgcc
     3841 ctaccgctgc ggaactgcgc tgttccgccg cgacctcccc cgcgtggccg cgctgaacgt
     3901 gctgcgtgtc gagagcggcg tcgaggcgct cgacgcggcg gccctgatcg agttcgccga
     3961 cgggctccag accgggctcc cgcagcgctc gctgcgggtg gtcgatgacc agctcgccgc
     4021 gcagctgcgc ccggtgttcg ccgccgcggg ctggatcacc gccggcatga cgctgatggc
     4081 cccgcgccgg cgtccggacc gcccgatcga cacgacctcg gtgcacgcgg tcgagctcga
     4141 gacgatcgcg tacgcgcgcc aggcgacgct cgggcgcgcc taccgcgacc tcgacacggc
     4201 cgagcagctc ctggtggcgg gtgagatgcc cgccctcggc accgaggtgc gtgccttcgc
     4261 ctcgaccgtg ggcaacgagg tggccgccta cgcggtggcg cgcgtcgcgg ggcacgtcgc
     4321 gaagctcacc gaggtcgacg cgctcgtgcg ctcccacggg cacggcctcg gccgcgcgct
     4381 gatctggggc gccgtgaagg agctgcgcgc cgagggcgtg cgtctcgtgg tggtcgaggc
     4441 ccaggacgac gcctggaccc gctggatcta cctacggctc ggcttcgagg acatcgggcg
     4501 cacgcaccgc ttcgtgcgtg catggggcga cgagcccgcc gcgccgcccg cgatctgacg
     4561 cgggcggcgg gcggggaccc gttcgctacg aggaggggct ggccgaggag gcgagctcct
     4621 gggcctgctg ctggccgctc tccttggccg tctcctgggc ggtctgagcg acctcctggg
     4681 cgacctgctt gccgcggtcg acggcctcgg acgcggtctc cttgacctgg ttcatgacct
     4741 ggtcggacat ctcgcccatc ttctcgtcct cgacgcgcgt ggacgggatc agcatgccgg
     4801 cgatgaagcc ggccgcgacc gcgccgatgg cgaggccgag cgggttctcc tgcgcgacgc
     4861 tcacggcctg ctgagcgccc tgcttcatct cgccggtgct cggcgtggcg tcgttcgccc
     4921 tgcccatcac accgccgacc ttcgacttga tcgcgtcggt cttctcggag acggagtcct
     4981 tcgcccgctc cttgacgtcg gtcttgtagc cgatcgcgcc gatggtctca cccatctgct
     5041 cgcgagtctg ctctatgtcc tggcggatgg cgtctgggtc ttggcccatt gcacgtcctc
     5101 cttcactgat tcgacggttt gctcgggtac gggcggggtc gcctgctgca cttccttctt
     5161 gcctgtgagg gcgaggaccc ccgcgatcgc ggcgtagacg agaccgacga tcagcgcggc
     5221 gagccacgcc ttcatgccgg tgtccagcgc caggatcagc gcggcggtca gtgcgcccag
     5281 ggccaggaag ccgacgacgc ccgcgccgcc gaacatgccc gctcccttgc ctgccttctt
     5341 gcccttctcc gccatctccg ccttggccag ttccagctcc tggcggacca gcgtggtcgt
     5401 ctcctccgag agctgcttga gcagctcgcc aatcggccgc tcccgcaggt cgtcgctcga
     5461 tccgttcgtc gccatccgcg ctcctagacc tgccccggcg tgacgcccgg cgcgaccggt
     5521 gcgcccgtgg gcggcggcgc gtagacctcc ggcgtggtgc cggagctgta gccgttcgac
     5581 ccggtcgacc cgctctggag ctggccgctc gcgcccgagc cggtctggct ccgctggctg
     5641 tagcgctggc tgctcgacgc cttcatgaac cgcgacgccg cgaagcccag cagcagcccg
     5701 ccggccacga cggcccacgg ctgcttgcga gcgaagtcct cgacgtcgct caggatcttg
     5761 tccgcgtcgg actcggtcag gtagctgccg accttctccg tgcgctcggc ggcctgctcg
     5821 gccagcttgg cgggggtctc cttgccctgc tcacggagcg tctcgccgac cgtgcgcaga
     5881 tcggaagcgg tcgtgctgac ctgctcgccg gcctgcttcg agcgctggtc gacctgctcg
     5941 cggaccttgc tgccggcctg ctgcttggcc tcctgcgcct tctcctgcgc ctggcccatg
     6001 acctcctggg ccttgtcctg cgccgcgtcc ttcgcctggc tcgcggtctc ctgcgcctgg
     6061 gctgcggcgc cgctctgctc gtcctgcgag ctcggcgcgg tagccccttg cgggggcgtg
     6121 cttacctcag ccatgccctc ctcctctgct cgggtcctct tgagcccaag cgctacccga
     6181 gggggcgacc caaaatcagt acgggtcccg agtggcgcat ggccgcgccg tggtgcgcgc
     6241 cctggcgcgc cggaccctgt ccgggagggt gctaggcggc cgcgctgtcg ccgccggcgg
     6301 gagcgtcgtc gctcgccgcg gaggcgtcct cgtcagcggc cgcgggggcg tcggcgtcgg
     6361 cggcggtcgc tggcgcggcg gcttcggcgt cgtcgccggc gggtgaagcg tcgtcgggcg
     6421 cgggagccgc gccggtcgcc gctggcgccg cggcttcgtc gccgtcgccc tcgtcgccgc
     6481 cgtcggcctc cgctgcctcg cgccgcagcc gcacctcgcg gcgctcgcgc ttggccggcc
     6541 gctcgtgatc ggtgccgggc tcctcgacgt gctcctcgtg ctcgccctcc tcggtcacgc
     6601 gcgcgggcgc catgaccatc gtcatgttgc ggccgtcgag gttggggcgc tgctcgatca
     6661 cgccgagctc gccgatgtcc tccgccaggc gcatcagcag ggcctcgccg cgctcgggat
     6721 gggtcgtctc gcggccgcgg aacatgatcg tgaccttcac gcggtcgttg tgcttgagga
     6781 agcgaacgac gtgacccttc ttggtctcgt agtcgttgtt cgcgatcttc ggccgcagct
     6841 tgatctcgcg gaccgtcacc tgcttctggt ggcggcgggc ggccttggcc ttctgctcct
     6901 gctcgtactt gtacttcgag tagtcgagca cgcgcacgac cggcgggcgc gcctcggcgg
     6961 ccacctcgac gagatcgagg tcgcgctcct gggcgtagcg cagggcctgg tccgtgggga
     7021 cgactccgac ctggttgccg tcgtcgccga tcagccgcac ctgcggcacg cggatccgct
     7081 cgttgatgcg agtctgatcg cgctccggcg ggcggcgatc gaacctacgt gggatcggca
     7141 ctagtgcggc cgtccgagcg ttcggtcggc ggggacccgg ctagacgtca aaggcgctct
     7201 cgtacgggag ggagcggcag cgacaggagg ggcaagggcg cgtcagcggg ggcgcggttc
     7261 gtgggtgctc ctcattcaca agcgagtata gccctcatcc ggtcgccggc tcgacccttc
     7321 cgcgggatcg tcaagagcgc gcggagatct cgtcgcgcag acgctcgatc accgcgtcga
     7381 ccggctcggc cccctggtcg ccctcgcggt ggcggcggac ggagacctgg ccggcctcct
     7441 gctccttgtc ccccacaacg agcatcagcg gcaccttgcg cagctcgccg tcgcggatct
     7501 tgcgcccgac cgactcggtg cggtcgtcca cctcgacgcg gatgccggcg ccacgcagcc
     7561 gctcctcgac ctcgcgggcg tagtcgttgt ggcggtcggc gatcggcagg acgagcgcgt
     7621 gcaccggcgt gagccacagc ggcagctcgc cggcgtagtg ctcgatcagg atgccgatga
     7681 agcgctcgaa cgagccgagc agcgcgcgat ggatcatcac cggccggtgc tcggcgttgt
     7741 ccgcgcccgt gtaggtgagc tcgaactgct cgggcatgta gtagtcgagc tggacggtgc
     7801 cgagctgcca cgagcgcttc agcgagtcgg tcatgtggag gtcgatcttc ggcccgtaga
     7861 aggcgccgtc gccctcgttc aggtcgtagt cgaggccgcg gctctcgagc gcccgggcga
     7921 gcgccgcctc ggcgcggtcc cacatcgcct cggtgccgac gcgcttctcg ggccgggtgg
     7981 agagctcgag tcgcggctcg aacccgaagt ggtcgtaaat gaagaagccg aagtcgaggc
     8041 agcgcaggac ctcctcctcg atctggtcct ccgtgcagaa cacgtgcgcg tcgtcctggg
     8101 tgatgtggcg cacgcgcagc agaccgtgca gcgtcccgct cggctcgtgg cggtgcacga
     8161 gcccctgctc ggcgtagcgg atcggcaggt cgcggtagga gcgcaggtcg cgcttgtaga
     8221 tctgaacgtg ggcgggacag ttcatgggct tgaggcccat gggccgctcc tccacgtccg
     8281 tgaagtacat gttgtcgcgg tagacgtgcc agtgccctga ctgcttccag agctcgacgc
     8341 cgtagaggat cggcgtgcgc acctcgacgt agccgcggcc gacgttctcg gtccgccaca
     8401 gcttggtcag ctcgttccag acgtgcgtgc cggcggggag ccagaacggc gagccgggcg
     8461 agaggtcgga gagcatgaac agcccgagct ctctgccgag cttgcggtgg tcgcgcgcgc
     8521 gggcctgctc gagcatctcg agatgctcgt cgaggtcctc cttgctcagg aacgccgtcc
     8581 cgtagatgcg cgtgagcatc tggcggttct cgtcgccgcg ccagtacgcg cccgcgaccg
     8641 aggtgagctt gaacgccttg atccgcttgg tcgtcgggcc gtgcggcccg cggcataggt
     8701 cggtgaaggg accgttgcgg tagagcgaga ccgtctcgac cgcctcgttc tggatcaggt
     8761 cctggatcag ctcgaccttg tacggctgct cctcgcgcgt gaagcggtcg agggcctcgg
     8821 cggtcgagac gtcgctgcgc tcgaaggact cgtcggcctt cacgtggcgg cgcatcgcct
     8881 cctcgatctt cgggaggtcg tcctccccca ccttcacgcc ctcggggaac tcgaagtcgt
     8941 agtagaagcc gtcctcgatc ggcggcccga tcgacacctt cgtgcctggg tagaggtcga
     9001 tcaccgcggt ggcgagcacg tgggccgtgt cgtggcggat cagctcgagg gcatccgggc
     9061 tcttgggcgt gacgatctcg atcggcttgc cgtcctcgag cggggccgac aggtccttca
     9121 gtgcgccgtt ctggcggata gccagcgctg cacgcgcaag gccctcacct atggcacggg
     9181 ccgcatccgc accggtggcc ccatcctcga gctcgagcgg agtgccgtcg gggagcgtga
     9241 cctgcatcgc gggaaagcta gcggccgtcc cggccgcgcc cggcgccgct gggcgccttc
     9301 gcccactgtg acctgccaca cagaacatgt gttcgctcgg ggtaccttca gccgagtaag
     9361 accggatcga cgacgcgggc cgtgggaggc cccatcgatc cccggaggtc gccagcgcag
     9421 ccgcgcaccc cgcggtcggc gaaggcggat tcggcgcccg cacgggcgag acgccgcgac
     9481 acagagcgcg gacgtcctcg cacgcgggct gagccaagtc acccgacccc ctccccggga
     9541 ggttcttcaa tgcgaaagct cttgctgtcc cttgcctgcg tcgccgcggc cgtgcccgcc
     9601 gtggcgctgg ccgccgacga ggacgacgtc caggtcagcg gcaccacgcc acaggccgac
     9661 gagcgccccg cggacgcgcg ccaaaaggcc aagcgcggcc aggcgcggat cgagcgcacg
     9721 cacgagcgtg ccgagaagcg ccgtcagcgc gcgcgggcgc gcaagcgtgc gcgggcgcgt
     9781 gcccgcgtaa atgccggcgg gtccggcggc ggtgccacgc cggcgcacct ccaggccatc
     9841 gcggcctgcg agtccggcgg caacccgagc gccgtcggcg gcggcggggc cttccgcggc
     9901 aagtaccagt tcgatcgcgg gacctgggcc tcggtcggcg gcagcggcga tccggccgcg
     9961 gccccggagg ccgagcagga tcgccgcgcg gcgatcctgt actcgcgggc ggggtcgagc
    10021 ccgtggccgg tgtgcggctc gtagacaact gcagcaccta gggagcagga cccggaggcc
    10081 cgccgagtgc gggcctccgt catcgattgg agggtttctc cgctatgcgg tacacaatgc
    10141 tccaatcgat gcgactcgtc gcgctcacgc tgctcgccgg ggtgcttgtc ggcacgatcg
    10201 gctgggcctc cgacgggggc ccgctgctcg tgcgctggac gagcgcgatc gccgcggggt
    10261 ggctgatcgg cgcgttcgtg gtgggcgcgc tcgccggcag cggcctgcgc gccgcggcgg
    10321 cgggcgcggg cacgatcgtc gccggcgtcg ccacctacta cgtgctcttc cacttcgtgt
    10381 ccggggagct gagcctgagg tacggcgtgg tcgtgggcgt cgcgtggtcg agcgtgggga
    10441 tcggcatcgg cgcggtgctc ggctgggccg gtgacgcgtg gcggcgccgg cacgccgatt
    10501 cgatcggcgt ggcgctcctc tcgggcgcgc tggccggcga ggcgatgctg ctgctcggcg
    10561 agtggcaaaa ccgcgcagcg cgggtcgtgc tgatctgcga gctcgtcctg ggcgcagccc
    10621 tgccatacct gctcgcacgc ccgaagctgg ccagaaccgt cgccctgagt gctgccgttg
    10681 ccgtcgccgt aataggcgta gagagcgccg tccgcgggac gatgcgcctc gcgggctggg
    10741 ccggcgccta gcccggcggg tccacgcgca cgacctggct gcccggcacg atgtcgtcgc
    10801 ggccggagtc ggtccagcgc acccaggcct tgtggccgcc gtcgtagtcg ggcgcccaca
    10861 gcacgagcgt cgcctcctcg tcccagaccg tgcagcgctc gtccgactcc tcgtcgacac
    10921 cacgccacac ccgcacgaac gcgttctgct cccactcgtc gccgatcgtg gtcggctcgg
    10981 tgtggcccgg gcgcacgact gtctcgggcg ggagcttcat cagcacgtcc atgatcgagg
    11041 ccttcaggtc ctcgaacgtc gtcgagccgg gcgcgcgcac gccgccgacg gagcccttga
    11101 acagcgtgtc gcccgtgtag atctccgcgt cgttcacgcg gaacgagagc atcccgtcgg
    11161 tgtgaccggg cgtgtgcagc ccgtcgatgc gcaggccgcc gacctcgagg acctcgccgg
    11221 gctcgatggc acggtccacg tccatcagcc gcgcggcctc gagcggatgg gccaggatct
    11281 tcacgccgag gcgctcctgg aacacgtggt tctcggagac gtggtcgtga tggtggtggg
    11341 tgagcagcag gtacgggatc gtcagctcgt tctgctccac cgcggcgagc aatggctcgg
    11401 ggtcggcccc gccgtcgacg atcactccgt gcccgcccgg ctcgtccgcg acgaggtacg
    11461 cgttggagag gaagtccggc gacatcgacc gctcgacgat cacggccggg aagatgacag
    11521 gatcgctcgg tggacgccgc cgccctccgc gccgagttcc ccgtgctggc gcagctcgcc
    11581 tacctgaacg ccgggaccca gggcccggtg ccggcccgag ccgccgccgc ggccaccgcc
    11641 accgttgagc acgagcgcga cgcggggcgc ggcgacaccg cgcacttcga ggcgctgttc
    11701 gccctcggcg agcggatgcg cgcgcacgtg gcgcgcctgt tcgcctgcga cgccgacgag
    11761 gtcgcgctga cccattcgac gaccgacggc atgaacgccg tgctgggagc gctcgacctg
    11821 ggcccgggcg acgaggtgct gacgagcgac gaggagcacc ccggcctgct cggcccgctc
    11881 gcgaccaacc gcgaccggcg cgggttcgac gtgcgcgtgg ccccgttcgc cgagatcgcg
    11941 aacgcgatcg ggccacgcac gaagctcgtg gcctgctcgc acgtctcgtg ggtgaacggc
    12001 aaggtggtcg acaccgcggc gctcgccgcc gccgacccgc tcgtgctgtt cgacggcgcc
    12061 cagggcctcg gcgcgctgcc gaccccgatg cgcgagctcg gctgcgactt ctacggcgcc
    12121 gccggccaga agtggctctg cggaccggac ggcaacggct acctctacgt gcgcggcgac
    12181 ctctccgcgg acctccccgc accgtggtcg agctacacga ccttcgccga tccgagcgac
    12241 ccgctcggat cgggcctcaa gcccggcgcg cggcgcttcg acctcggctt ctcggcgccg
    12301 cactggtcct ggtcggtcgc cgcgctcgac ctgctggccg acgccggctg ggacgagctg
    12361 ctcgcgcgcg gaccggcgct ggccgcgcgg ctggccggcg agctcaccga gcgcggagtg
    12421 caggtcgagc cgcgcgggcc ctcgacgctc gtctcctggc gctccgagga cgaagccgcg
    12481 gagacgcggc ggctcgcgga cgcgggcgtc cgggtgcgcg acatcccggg gcacggcctg
    12541 gtgcgcgcct cggtgggcgc gtggtcgagc gaggaggacc tagagcgcct gctggcggcg
    12601 cgctagcagc cggcggactg cgcggcgcgg acggccgcgc gcgggccgac gtacggcgcc
    12661 agcgcgatgt agacgaggtc cggcgtcatt cgcgggagct cgcgcacccg gccgtggaag
    12721 atctcgtcgt agatcagcga gtagagcgcg ccgaccaccg cctgcgccgt cagctccggg
    12781 atcgtgagcc ccgccggcgc ctcgtggcgg cccggcgcaa gcagctcggt gaagtgctgg
    12841 aggaccgcgt cgcggcgctc gagcgcacgc ggcccggcgg cgagtatctc gatgatgcag
    12901 aagcgggcca gcgcggggcg cgaggccagc cgctcgagca ggatccccag cgccgaggcg
    12961 acgcgctccg cccacgccgg ccggccctcg tacgcctcga ccaccgagcc catgagctgg
    13021 cgcgtgatct cgtcgtacgc ggcgatgaag cactcctcct tgtcgctgaa gtgctcgtag
    13081 aacgtcttcc gcgagacggc ggcctgcccg atcacgtcgg cgaccgtcgt tgcggcgtac
    13141 cccttggcgg ccacgcgctc gatcacggcg tcgatcatcc gctcgcgctg cgagcggagg
    13201 acgacctcac gcgggaggtt gtggcgcccg cgtggcagct cgtgcgcgag gcgcagcggt
    13261 gcctcggaag gactcactcc ctggacagta cctacgcccc aagaacagaa acatgggggg
    13321 gttatccaga aacaccaaag gggtagctgg aaacatcggc gtttaagaag acgccgaaac
    13381 agacagcgcc gggaacgcca agccgaactg gaagcccgcg aggcgccccg gcaccgcata
    13441 gaacagcgta gaggcgcctg ccatggccgg caacgccgct gcgggagagg caggagagaa
    13501 tgaaggtacg tacgaaggcc gccgccgcga tctgcggtgc gattgccacc gtcgcgatca
    13561 cggccatggc ggcctgggca gcgggatcga ccacgatcac gccggccggc gactacacca
    13621 aggcgtcgct caagtccggg acgaacgccc agttcaccat cgccggcttc ctgtcggtga
    13681 cgtgcaacca gtcgacgagc gcgccgacct cgccgcttgg gaccaacacg aagaaccaga
    13741 tcccgccggc gcccggcaac cacaacgcca gcggggccgt caccctgccg gtgaacgcgc
    13801 cgagcttcac caacaacggc ggggcgtgcc ccaccagcct gtccggtgtc acggcgacca
    13861 cgaccaccag cggaagctgg accctgtcgg gccagaacgg ctcgccgatc acgggctcgc
    13921 tcacgatccc gaaggccggt gccgtgacca gcacgagcaa cggttgcacg atcaccgtcg
    13981 ccccgagcgg cccggtgagc gtcggcggga cgtgg
//
LOCUS       DASVPL010000132        11677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_329426, whole genome shotgun sequence.
ACCESSION   DASVPL010000132 DASVPL010000000
VERSION     DASVPL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329426"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VF517_06810"
     CDS             <1..1378
                     /locus_tag="VF517_06810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8052685.1"
                     /translation="ERLAEALRRRAEEPDLRGRVTFAGSLDDPRPALAESTCLLHAAD
                     VEPYGMVVVEALAAGRAVVAPASGGPSEIVAPETGRLFVPGDPHSAADALVDLLGADG
                     LAARVGAAGRARAERHYRVEDSTARYAALLDELAERRRAGSATAVPRAPAAPDPSLPG
                     TGVAIVTVLHDSEPEVRALLASVERHLPGARVVAADSGSSDCGPAAVRDWSGGATLVE
                     LGENVGFGRASNEALAAVEEPVTVILNPDAELLDASLADLAAEVTREGRPERILAPLV
                     VQPDGLRENSAHPEPGSVPEFLRALVPGAALPRPLRAPIEPWRADTPRRVGWAVGCCL
                     VARTDTLRRLGPFDDRTFLYAEDLDLGLRATDAGIETWFWPEGRVLHRGAHASARRFG
                     GEPFDLLAARRREVVRRWRGERRQRADDAAQAATFATRIALKSALGGTPAREREQLAA
                     LRRARRAG"
     gene            complement(1382..2539)
                     /locus_tag="VF517_06815"
     CDS             complement(1382..2539)
                     /locus_tag="VF517_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX8052686.1"
                     /translation="MTRSALLAVAAACLALAALPATAGAQKRLVPPLFFGANWDGELE
                     FNSPESVREAETARMARSGVESVRVPFEWVRAQPQQGMPIDHTITDRFVRHAAAHNID
                     IVPIVILAPKWARQYENVTHSPPKGTGGYTGYLRSLIARYGPNGTFWTENPLLAKHPI
                     RNWQIWNEPHLQFQWSIPTGDDYAPGYGKLLRASYKVVKAADPGARVVLGGISNESWK
                     YLRHMYEKGRIKGAFDVAALHPYTSKADGIVTLTKRFRIVLRDNGDAKRPLWITEVGL
                     PASKGRINSKNKLQTTDEGMADYLVRAYQVIVKNRSSSLAKVARVYWYNWASVYCCEQ
                     FRFTGLLQYDNKETVTPKPAFEQYVRMAQRYEGCAKDDQARCVEPPAATPR"
     gene            2625..4286
                     /locus_tag="VF517_06820"
     CDS             2625..4286
                     /locus_tag="VF517_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8052687.1"
                     /translation="MATVETSNPAREVLNQPPVLGGRNLFTENVPLLEGVRREGADHS
                     VERLTALGAASGEAADRELGRLANENEPVLHTHDRFGHRIDEVEFHPAWHELLELSVG
                     HGTHALPWREPGPGAHVARAAMMLCGSGLESGHGCPISMTYAAVPVLRRQPELAAEWE
                     PRLTSLDYDRRFVPADRKTGALAGMAMTERQGGSDVRANTTLARPLNGGGPAAEYELT
                     GHKWFCSAPMCDMFLVLAQADGGLSCFLLPRWTPDGERNRFHIQRLKDKLGNRSNASS
                     EIELDGAWARMVGEEGRGVPTIIEMVNFTRLDCVLGSTGLMREALVQAIHHCSHRTAF
                     GNLLADQPLMRNVLADLAIESEAATLTALRLARAVDEGPDDPEQTLFKRLGLAVSKYW
                     ICKSATWHVAEALECLGGNGYVELSGMPRIHREAPLLSIWEGSGNVNCLDVLRAMGRT
                     PEAVHAFFDEVESAAGAEPRLDAYVRELKDELSDPSDIELRARRIVERMGVALQASLL
                     VRHGDAAVADTFCASRLAGDRGRALGTLPGGVDFERIIERHSAAR"
     gene            4313..5263
                     /locus_tag="VF517_06825"
     CDS             4313..5263
                     /locus_tag="VF517_06825"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8052688.1"
                     /translation="MEASAAGAERFEQPGRDDIEPVANETRDRIITGIVTGLPFAGLG
                     LVVWQAWSNSLRGSDVVVFAIMYSLTGLGITVGFHRYLTHRSFKTSRPVRMVLGILGS
                     AAIEGPVTAWVADHRKHHAFSDQEGDPHSPHVGHGGGWRGALRGLVHAHVGWLFLHTQ
                     RGNKRRYAPDLMNDPTIAFVDRTFLVWALGGLALPFFLGMAIGGSLHAGLTGLLWGGL
                     VRVLVLHHVTYSINSLCHFFGRRGFATGDESRNLAWLAPLSFGEAWHNNHHAFPTSAA
                     HGLRRTQLDPSALVIRGLERVGLAWDVVRVSPERMGTKAL"
     gene            5260..6156
                     /locus_tag="VF517_06830"
     CDS             5260..6156
                     /locus_tag="VF517_06830"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8052689.1"
                     /translation="MTLNYEVDGRIARITLNRPERGNGITLEMPRELAAAVERADLDP
                     AVHVIALSGAGKGFCGGYDLVASAEQRMEGVGGGADAPEGSPLDPLVQLRNHDPAGTW
                     DPIVDYAMMSRNVRGFMSLFHADKPVVCKVHGFCVAGGTDMALCSDLLVIADDARIGY
                     PPARVWGTPTTMVWAHRIGPEKAKRLLFTGDLLTGREAHEWGLAIESAPPEELDARFE
                     ALLERIALVPVNQLVMHKLLVNQTLHAQGLQTTQTLATVFDGVARHTPEGFAFQQRAA
                     EAGFKEAVRERDEPFGDAPPPR"
     gene            complement(6153..7670)
                     /locus_tag="VF517_06835"
     CDS             complement(6153..7670)
                     /locus_tag="VF517_06835"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX8052690.1"
                     /translation="MRRIALLTTLLVALLAAAPAHAANVALFNDTTYVGNQESANMQA
                     VLVSQGHSVFTFTDESAAGIASALEGRTALIIPDLAGGTDLHTALSGSAREVIRQFVE
                     NGGGFVINGRATGDEAANLLNTLFNFELTESPTANPAARNQTAAGTEYAAGPDTLPSN
                     NTTSGIDLASLPANANQVYVSAGQAHVAVMYRGLGSITYLGWDWNNSNPPTPGGQDGG
                     WQGILNSAVSAPTVNVSDVGVAEGDTGVTTARFPITLAGGPVSEDVRVTFELTGGNAT
                     AGQDYVVPATTVTVPRGQTVAFVEVPVVGDAADEPNETFELHVTSVTAAAVGRGTALG
                     TIGDDDPSPGRCANRQNANDTGDKLTGTAFGDLLVGGAGKDILSGGGGDDCLRGRAAG
                     DRLSGGDGNDSLSGEAGSDSLSGGEGKDSLSGGDGNDVLSGGNGNDRLGGGAGNDKIN
                     GGQGLNTFSGGAGNDNIVAANGRRESIDCGSGKRDKVRADRTDNVRNCETVQRLS"
     gene            complement(7728..8753)
                     /locus_tag="VF517_06840"
     CDS             complement(7728..8753)
                     /locus_tag="VF517_06840"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEX8052691.1"
                     /translation="MHAADPLAEFYATTPREPSGLSGRAGVEGAVDAALEELRVPATL
                     DEDGDWTLETDVGQLAVIADDLTGDVVAVQTLQSMDRSVEDSAEEMHALLRLNFEFRG
                     LARFGAITQGGKHLLVITARIAGDEVSTETVQRLLADTLRLSRRLDELLGRAPAEPAT
                     ASAAEPEAAEAAPAEPAPVADPDVTAFAPVPGPLPPSVGEPEVGEPGVAEQGAAEPDA
                     AEPGVAEPAAETVFAQPVPQAPPEPEPAPVAEPAPELVAESAPVAEPAPLAEYPPPEP
                     PAPASPEPSYPPPGAEPQQAQPTYPPPVYSPPEPELPPANWYPDPYYEARLRYWDGQQ
                     WTQHVAQ"
     gene            complement(8764..9843)
                     /locus_tag="VF517_06845"
     CDS             complement(8764..9843)
                     /locus_tag="VF517_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052692.1"
                     /translation="MSAQPPLREPPPAQPGPLGGPPGGSVGSPAASVGLPGGVFGPPG
                     GLGAPALAPLVAAAPLVPALPLGGVPVGAAPGGERPGVPERPAAGPPSFASGPPSSAA
                     GGFGGAPLPGGAAPGGAAPLALPDLEGAARAAMASVLERDAPAGSGRRAPDVIELAHQ
                     ARLETVAGAPTEPDPRQVTGAIGFQTPHVAPLTPVRAGGPVGQGLPLRQGLAPARGLP
                     PGRGLTPAQASAPGQALAPRQGLPPAPPPPAAGVWRPPPPPVWRHPPRQAPPAQPLDR
                     SSRPDAALVPGERDAAQRVGDALYPFVAWLREGRSGWFGVAALFGITGPIVLLRQNFD
                     VRLRLVLSFFVAIFWIQLLSGLFGS"
     gene            complement(9916..10332)
                     /gene="atpC"
                     /locus_tag="VF517_06850"
     CDS             complement(9916..10332)
                     /gene="atpC"
                     /locus_tag="VF517_06850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX8052693.1"
                     /translation="MAHKTFPVEVLTPEGKAFEGEVEMLSTRTNTGSIGIRANHAPLM
                     AILDPTELRLYEESEGEPKRFAQGEGYLQVVNNSALVLVDEILDPDSINSSDIESRLR
                     EAEQALDQADEDTEEYRRAERNVRRYKAFLEVTQGG"
     gene            complement(10340..>11677)
                     /gene="atpD"
                     /locus_tag="VF517_06855"
     CDS             complement(10340..>11677)
                     /gene="atpD"
                     /locus_tag="VF517_06855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX8052694.1"
                     /translation="GLSRGTEVRNTGKPISVPVGEVTLGRIFNLLGEPIDEGDEIEDD
                     AERWPIHRPVPTVEDLTPTREIFETGIKVVDLLAPYAKGGKVGLFGGAGVGKTVLIQE
                     LINNIASEHGGLSAFCGVGERSREGNDLWLEMKESGVIDKTMLVFGQMNEPPGARMRV
                     ALSGLTMAEYFREEGGQDVLLFIDNIFRFVQAGSEVSALLGRMPSQVGYQPTLETEMG
                     QLQERITSTRRGSVTSVQAIYVPADDLTDPAPASVFAHLNATTVLSRSIAEKGIYPAV
                     DPLDSTSTILKADIVGEEHYQVATEVKEVLQRYKELQDIIAILGIDELSDEDKQTVQR
                     ARKIERFLSQPFFVAEAFTGSPGTYVKVEDTIRGFKEIVEGKHDDIPERAFYMKGTID
                     EVVEAARSEGGSGSGSGSDSGSEDSGNGGGEDEDSERGEGSEDSQDAVDASGQ"
BASE COUNT         1479 a         4334 c         4219 g         1645 t
ORIGIN      
        1 cgagcgcctc gccgaggccc tgcggcgccg cgccgaggaa cccgacctgc gcggccgggt
       61 cacgttcgcg ggctcgctcg acgacccgcg accggcgctc gccgagtcga cctgcctgct
      121 ccacgccgcc gacgtcgagc cctacgggat ggtggtggtc gaggcgctgg ccgcgggccg
      181 cgccgtggtg gcgccggcat ccggcgggcc gtccgagatc gtcgcgcccg agaccgggcg
      241 gctgttcgtg ccgggcgacc cgcactcggc cgccgatgcc ctcgtggacc tgctcggcgc
      301 cgacggcctg gccgcgcgcg tgggcgccgc cggccgcgcg cgggccgagc gccactaccg
      361 cgtcgaggac tcgacggcgc gctacgcggc gctgctcgac gagctcgcgg aacgccgccg
      421 cgcgggctcc gcgacggcgg tgccgcgggc cccggccgcg cccgacccct ccctccccgg
      481 caccggggtc gcgatcgtca ccgtgctcca cgactccgag cccgaggtgc gggcgctact
      541 cgcatcggtc gagcgccacc tgcccggcgc gcgcgtggtc gcggccgact ccggctcgag
      601 cgactgcggc cccgccgcgg tgcgcgactg gagcggcggc gccacgctcg tcgagctcgg
      661 cgagaacgtg ggcttcggcc gcgcctcgaa cgaggcgctc gcggccgtgg aggagcccgt
      721 caccgtgatc ctgaacccgg acgccgagct gctcgacgcg tcgctcgcgg acctcgcagc
      781 ggaggtcacg cgcgaggggc gtccggagcg catcctcgcg ccgctcgtgg tgcagccgga
      841 cggcctgcgc gagaactcgg cccaccccga gccgggctcg gtgccggagt tcctccgggc
      901 gctggtcccg ggcgcggcgc tgccgcgccc cctgcgcgcg ccgatcgagc cgtggcgcgc
      961 cgacacgccg cgccgggtcg gctgggccgt tggctgctgc ctcgtcgcgc gcaccgacac
     1021 gctgcgccgg ctcggcccgt tcgacgaccg gaccttcctc tacgccgagg acctcgatct
     1081 cggcctgcgc gccaccgacg ccggcatcga gacctggttc tggccggagg gccgggtgct
     1141 gcaccgcggc gcgcacgcgt cggcgcggcg cttcggtggc gagccgttcg acctgctggc
     1201 cgcccgccgc cgcgaggtgg tgcgccgctg gcgcggggag cgccgccagc gcgccgacga
     1261 cgccgcccag gccgccacct tcgccacccg catcgccctc aagtccgcac tcggcggcac
     1321 ccccgcccgc gagcgcgagc agctcgccgc cctgcgccgg gcgcggcgcg ccggctgagc
     1381 ctcagcgcgg cgtggccgcg ggcggctcga cgcagcgagc ctggtcgtcc ttcgcgcagc
     1441 cctcgtagcg ctgcgccatc cgcacgtact gctcgaacgc cggcttcggc gtgacggtct
     1501 ccttgttgtc gtactggagc aggccggtga agcggaactg ctcgcagcag tagaccgagg
     1561 cccagttgta ccagtacacg cgggccacct tcgcgaggct cgacgagcgg ttcttgacga
     1621 tcacctggta ggcgcggacc aggtagtcgg ccatcccctc gtcggtggtc tggagcttgt
     1681 tcttcgagtt gatccggccc ttcgacgccg gcagcccgac ctccgtgatc cagagcgggc
     1741 gcttggcgtc gccgttgtcg cgcagcacga tccggaagcg cttcgtgagc gtgacgatcc
     1801 cgtcggcctt cgaggtgtag gggtgcaggg ccgccacgtc gaacgcgccc ttgatccggc
     1861 ccttctcgta catgtgccgg aggtacttcc acgactcgtt cgagatgccg ccgagcacca
     1921 cgcgcgcacc cgggtcggcc gccttcacga ccttgtacga cgcgcgcagg agctttccgt
     1981 agccgggcgc gtagtcgtcg ccggtcggga tcgaccactg gaactggaga tgcggctcgt
     2041 tccagatctg ccagttgcgg atcgggtgct tggcgagcag cgggttctcg gtccagaacg
     2101 tgccgttcgg cccgtagcgc gcgatcagcg agcgcaggta gcccgtgtac ccgcctgtcc
     2161 ccttcggcgg cgagtgggtc acgttctcgt actggcgggc ccacttcggc gcgaggatca
     2221 cgatcgggac gatgtcgatg ttgtgcgcgg cggcgtggcg cacgaagcgg tccgtgatcg
     2281 tgtggtcgat gggcatcccc tgctgcggct gcgcgcgcac ccactcgaac gggacgcgca
     2341 ccgactcgac gccgctgcgg gccatccggg cggtctcggc ctcgcgcacg ctctcgggcg
     2401 agttgaactc cagctcgccg tcccagttgg cgccgaagaa gagcggcggc accagccgct
     2461 tctgcgcgcc ggcggtggcg ggcagcgcgg cgagcgcgag gcacgcggcc gcgacggcga
     2521 ggagggcaga gcgggtcacg accacgagaa cccgagagag tacgaacggt tgcggcgcgc
     2581 cacgcggtcg acttcgcggc gctggcgcgt aggatctagc cctgatggcc accgtcgaga
     2641 cgtcgaaccc ggcgcgcgag gtcctgaacc agccccccgt cctcgggggc cgcaacctct
     2701 tcaccgagaa cgtgccgctc ctcgagggcg tccgccgcga gggcgccgac cactcggtgg
     2761 agcgcctcac ggcgctgggc gccgcgtccg gcgaggccgc cgaccgcgag ctcggccgcc
     2821 ttgccaacga gaacgagccg gtgctccaca cccacgaccg cttcggccac cggatcgacg
     2881 aggtggagtt ccacccggcc tggcacgagc tgctcgagct ctcggtcggg cacggcaccc
     2941 acgcgctgcc ctggcgcgag ccggggcccg gcgcccacgt cgcgcgcgcg gcgatgatgc
     3001 tctgcggcag cgggctcgag tccggccacg gctgcccgat ctcgatgacc tacgccgccg
     3061 tgcccgtgct gcgccgccag cccgagctgg ccgccgagtg ggagccgcgc ctgacgtcgc
     3121 tcgactacga ccgccgcttc gtgccggccg atcggaagac gggcgccctc gccgggatgg
     3181 cgatgaccga gcgccagggc ggatccgacg tgcgcgcgaa caccacgctc gcccgcccgc
     3241 tgaacggcgg cggcccggcc gcggagtacg agctcaccgg ccacaagtgg ttctgctcgg
     3301 cgccgatgtg cgacatgttc ctggtgctcg cgcaggccga cggcgggctc tcgtgcttcc
     3361 tgctgccgcg ctggacgccc gacggcgagc gcaaccgctt ccacatccag cggctgaagg
     3421 acaagctcgg caaccgctcg aacgcgtcga gcgagatcga gctcgacggc gcctgggcgc
     3481 ggatggtcgg cgaggagggg cgcggcgtcc ccacgatcat cgagatggtc aacttcacgc
     3541 ggctcgactg cgtgctcggc tccaccggcc tgatgcgcga ggccctcgtc caggcgatcc
     3601 accactgctc gcaccgcacg gcattcggca acctgctggc cgaccagccg ctgatgcgaa
     3661 atgtcctggc cgatctcgcg atcgagtccg aggccgccac gctcacggcc ctgcggctgg
     3721 cgcgcgcggt cgacgagggg cccgacgatc ccgagcagac gctgttcaag cggctcggcc
     3781 tggcggtctc gaagtactgg atctgcaaga gcgcgacatg gcacgtcgcg gaggcgctcg
     3841 agtgcctcgg cggcaacggc tacgtcgagc tctccggcat gccgcgcatc caccgcgagg
     3901 ccccgctcct ctcgatctgg gaggggtcgg ggaacgtgaa ctgcctcgac gtgctgcgcg
     3961 cgatggggcg cacgcccgag gcggtgcacg cgttcttcga cgaggtcgag tcggccgccg
     4021 gcgcggagcc gcggctcgac gcctacgtgc gcgagctgaa ggacgagctg agcgacccgt
     4081 ccgacatcga gctgcgcgcc cgccggatcg tcgagcgcat gggcgtggcg ctccaggcct
     4141 cgctgctcgt gcgccacggg gacgccgcgg tggccgacac gttctgcgcc agccgcctcg
     4201 cgggcgatcg cggccgcgcg ctcggcaccc tgcccggcgg cgtggacttc gagcgcatca
     4261 tcgagcgcca cagcgccgct cgctgagaat tcgccgctag gctccgtccc acttggaggc
     4321 ctctgccgcc ggagcggaac ggtttgaaca gccgggacgc gacgacatag agccggtcgc
     4381 gaacgagacg cgcgaccgga tcatcaccgg catcgtgacc gggctgccct tcgcggggct
     4441 gggactcgtg gtgtggcagg cgtggagcaa ctccctgcgc ggaagcgacg tcgtcgtgtt
     4501 cgcgatcatg tactcgttga cggggctggg gatcaccgtc ggcttccacc gctacctgac
     4561 gcaccgcagc ttcaagacca gcaggccggt gcgcatggtg ctcgggatcc tcggctcggc
     4621 cgccatcgag ggcccggtga ccgcctgggt ggccgaccac cgcaagcacc acgcgttctc
     4681 ggaccaggag ggcgatccgc acagcccgca cgtcgggcac gggggcggct ggcgcggtgc
     4741 tctgaggggg ctcgtgcacg cgcacgtcgg ctggctcttc ctccacaccc agcgcggcaa
     4801 caaacgccgc tacgcgccgg acctgatgaa cgaccccacg atcgcgttcg tcgaccggac
     4861 cttcctcgtc tgggcgctcg gcggcctggc gctgccgttc ttcctcggga tggcgatcgg
     4921 aggctccctc cacgcgggac tcaccggcct gctgtggggc ggactcgtgc gggtgctcgt
     4981 gctccaccac gtgacctaca gcatcaactc gctgtgccac ttcttcgggc gccgcgggtt
     5041 cgcgaccggg gacgagtcgc gcaacctggc gtggctggcg ccgctgtcgt tcggcgaggc
     5101 gtggcacaac aaccaccacg cgttcccgac ctccgcggcg cacgggctgc gccgcacgca
     5161 gctcgacccg tccgcgctcg tgatccgcgg tctcgagcga gtcgggctcg cgtgggacgt
     5221 cgtccgggtg agcccggagc ggatgggtac caaggcgctg tgacgctgaa ctacgaggtc
     5281 gacgggcgga tcgcgcggat caccctgaac cgccccgagc gcggcaacgg catcacgctc
     5341 gagatgccgc gcgagctggc ggccgcggtg gagcgggcgg atctcgaccc cgccgttcac
     5401 gtgatcgcgc tgtccggcgc cgggaagggc ttctgcggcg ggtacgacct ggtcgcgagc
     5461 gcggagcagc ggatggaggg cgtgggcggc ggcgccgacg cacccgaggg ctcgccgctc
     5521 gacccgctcg tgcagctgcg caaccacgac ccggcgggca cctgggaccc gatcgtcgac
     5581 tacgcgatga tgagccggaa cgtccgcggc ttcatgtcgc tcttccacgc ggacaagccg
     5641 gtcgtctgca aggtgcacgg gttctgcgtg gccggcggga ccgacatggc cctctgctcg
     5701 gacctcctgg tgatcgccga cgacgcgcgc atcggctacc cgcccgcgcg ggtgtggggc
     5761 acgccgacca cgatggtctg ggcgcacagg atcgggcccg agaaggcgaa gcggctgctg
     5821 ttcaccggcg acctgctgac gggccgcgag gcgcacgagt gggggctggc gatcgagtcg
     5881 gctccgccgg aggagctcga cgcccggttc gaggcgctgc tcgagcggat cgcgctcgtg
     5941 cccgtgaacc agctcgtgat gcacaagctg ctcgtgaacc agacgctgca cgcccagggg
     6001 ctccagacga cgcagacgct cgccacggtc ttcgacggcg tggcgcgcca cacgcccgag
     6061 ggcttcgcgt tccagcagcg cgccgccgag gcggggttca aggaggccgt gcgcgagcgc
     6121 gacgagccgt tcggcgacgc cccgccgccg cgctagctca ggcgctgcac ggtctcgcag
     6181 ttgcggacgt tgtcggtgcg gtccgcgcgc accttgtccc tcttcccgga gccgcagtcg
     6241 atcgactcgc ggcggccgtt ggcggcgacg atgttgtcgt tgccggcgcc gccgctgaag
     6301 gtgttgaggc cctgcccgcc gttgatcttg tcgttgccgg cgccgccgcc gaggcggtcg
     6361 ttgccgttcc caccggagag cacgtcgttg ccgtcgccgc ccgacagcga gtccttgccc
     6421 tcgccgccgc tgaggctgtc gctgccggcc tcgccgctga gggagtcgtt cccgtcgccg
     6481 ccggagaggc ggtcgccggc cgcgcggccg cgcaggcagt cgtcgccccc gccgccgctg
     6541 aggatgtcct tgccggcgcc gcccaccagc aggtcgccga aggccgtgcc ggtcagcttg
     6601 tcgccggtat cgttggcgtt ctggcggttg gcgcagcgac cgggtgaggg atcgtcgtcg
     6661 ccgatcgtgc cgagggcggt gccgcgcccg accgccgcgg cggtcacgga cgtgacgtgc
     6721 aactcgaagg tctcgttggg ctcgtcggcc gcgtcgccga ccacggggac ctcgacgaac
     6781 gccacggtct ggccgcgcgg gaccgtcacg gtcgtcgcgg gcacgacgta gtcctggccc
     6841 gcggtggcgt tgccgccggt cagctcgaag gtcacgcgca cgtcctcgga gaccggcccg
     6901 cccgcgaggg tgatcgggaa gcgagcggtg gtcacgccgg tgtcgccctc ggccacgccg
     6961 acgtcgctca cgttcaccgt cggcgccgag acggcggagt tcaggatccc ctgccagccg
     7021 ccgtcctggc cgccgggcgt gggcgggttc gagttgttcc agtcccagcc gaggtaggtg
     7081 atcgacccga gcccgcggta catcaccgcg acgtgggcct ggcccgcgct cacgtagacc
     7141 tggttcgcgt tcgcgggcag gctcgccagg tcgatgccgg aggtcgtgtt gttggagggg
     7201 agcgtgtcgg ggccggcggc gtactcggtg ccggcggcag tctggttgcg cgcggcgggg
     7261 ttcgcggtgg gcgactcggt cagctcgaag ttgaagaggg tgttcaggag gttcgcggcc
     7321 tcgtcgccgg tggcgcggcc gttgatcacg aagccgccgc cgttctcgac gaactggcgg
     7381 atgacctcgc gcgcgctgcc cgacagggcg gtgtggaggt cggtgcctcc ggcgaggtca
     7441 gggatgatca gtgccgtgcg gccctcgagg gcgctggcga tgcccgccgc ggactcgtcg
     7501 gtgaacgtga agacgctgtg gccctgcgag accaggaccg cctgcatgtt ggccgactcc
     7561 tggttgccta cgtaggtcgt gtcgttgaag agcgcgacgt tggcggcgtg cgcgggcgcg
     7621 gccgcgagca gcgcgacgag cagggtcgtg agcagtgcga tccttcgcat cgaacgtgca
     7681 ttcgcggcgg gccggccaga cccttcccgg gtcggcgcgt cgcctgccta ctgggccacg
     7741 tgctgagtcc actgctgccc gtcccagtac cgcagccggg cctcgtagta cgggtccggg
     7801 taccagttgg cgggcggaag ctccggctcg ggcggcgagt agacgggcgg cggataggtc
     7861 ggctgtgcct gctgcggctc cgcgccgggc ggcggatagg acggctcggg ggaggcggga
     7921 gcggggggct ccggcggcgg gtactccgcc aggggggcgg gctccgccac gggggcggac
     7981 tcggccacga gctccggtgc gggctccgcc acgggcgcgg gctccggctc gggcggcgcc
     8041 tgcggcaccg gctgcgcaaa gacggtctcg gccgccggct cggccactcc cggctccgcc
     8101 gcatccggct ccgccgctcc ctgctcggcc actcccggct cccccacttc cggctcccca
     8161 acactcggcg gcaagggccc aggcaccggg gcgaatgcgg tcacgtccgg gtcggccacg
     8221 ggggcgggct ccgccggtgc ggcttcggcc gcctcaggct ccgcggcgct cgccgtggcc
     8281 ggctccgccg gagcccgtcc caggagctcg tccaggcggc gcgagaggcg cagcgtgtcc
     8341 gcgagcagcc gctgcacggt ctccgtcgag acctcgtcgc ccgcgatccg cgcggttatc
     8401 accagcaggt gcttcccacc ctgggtgatg gcgccaaagc gggcgagccc gcggaactcg
     8461 aagttcaacc gcagcagcgc gtgcatctcc tcggcggagt cctcgacgga gcgatccatc
     8521 gactggagcg tctgaacggc gaccacgtcg ccggtcagat cgtcggcgat caccgcgagc
     8581 tgccccacgt cggtctccag tgtccagtcg ccgtcctcgt cgagcgtcgc cggcacgcgc
     8641 agctcctcga gtgccgcgtc caccgcgccc tccactcctg cgcggccgct caagccgctc
     8701 ggctcgcgtg gcgtcgtggc gtagaactcg gcgagtggat cggcggcgtg catcgcgcgc
     8761 agcctagctt ccgaagagcc ccgacaggag ctggatccag aagatcgcga cgaagaacga
     8821 gagcaccagc cgcaggcgca cgtcgaagtt ctgccgcagc agcacgatcg gtcccgtgat
     8881 gccgaacagc gccgccaccc cgaaccaccc cgaccgaccc tcgcgcagcc acgccacgaa
     8941 ggggtagagg gcgtccccga cccgctgcgc cgcatcgcgc tcgcccggca cgagcgccgc
     9001 gtccggccgg ctcgagcggt cgagcggctg cgccggcgga gcctgccgcg gcgggtgccg
     9061 ccacaccggc ggcggcgggg gccgccacac gccggcggcc ggaggcggcg gtgccggcgg
     9121 gaggccctgc ctcggcgcca gcgcttgccc cggcgcggac gcctgcgctg gcgtgagacc
     9181 ccgtcccggc gggagccccc tggcgggcgc gagcccctgc ctcagcggga gcccctgccc
     9241 caccggccca ccggcgcgca ccggcgtcag cggggccacg tgcggcgtct ggaagccgat
     9301 cgctccggtc acctgtcgcg gatccggctc cgtcggtgcg cccgccacgg tctcgagccg
     9361 cgcctggtgc gcgagctcga tcacgtccgg cgcacgccga cccgaacccg caggcgcatc
     9421 gcgctccagc acgcttgcca tcgccgcccg cgcggcaccc tcgaggtccg gcagggcgag
     9481 cggagccgcg ccgcccggag cggccccgcc gggcaggggc gccccgccga agccgccggc
     9541 ggcggagctc ggcggtcccg aggcgaagct cggcggcccc gcagcgggcc gctccggcac
     9601 ccccggtcgc tcccctccgg gcgccgcccc caccggcacg ccgcccagcg gcagcgccgg
     9661 aaccagcggc gcggcggcca cgaggggcgc cagcgccggc gcacccagcc ccccgggcgg
     9721 tccgaagacc ccgcccggca gcccgacgga cgcggcgggc gacccgacgg accctcccgg
     9781 cggcccgcca agcggccccg gctgtgccgg aggcggctcc cgaaggggcg gctgcgcgct
     9841 cattcgcgca gcctacggca gcagcattcc tgacttatgg gtccaggaga cccaaaagtc
     9901 aggaaagcct gaggatcaac ctccctgcgt gacctcgagg aaggccttgt agcggcgcac
     9961 gttgcgctcg gcgcggcggt actcctcggt gtcctcgtcg gcctggtcga gcgcctgctc
    10021 cgcctcgcgc agccggctct cgatgtccga gctgttgatc gagtccgggt cgaggatctc
    10081 gtcgaccagc acgagcgcgc tgttgttgac gacctggagg tacccctcgc cctgcgcgaa
    10141 gcgcttgggc tccccctcgc tctcctcgta gagccgcagc tcggtcgggt cgaggatcgc
    10201 catcagcggc gcgtggttgg cgcggatgcc gatcgatccg gtgttcgtgc gcgtggagag
    10261 catctccacc tcgccctcga acgccttgcc ctcgggcgtc agcacctcga ctgggaacgt
    10321 cttgtgcgcc atagaccggc tactgccccg acgcgtccac ggcgtcctgc gaatcctcgg
    10381 acccctcgcc gcgctccgag tcctcgtcct caccgccacc gttgccggag tcctcggagc
    10441 cggaatcgga ccccgacccc gagcccgagc cgccctcgct ccgcgccgcc tcgaccacct
    10501 cgtcgatcgt gcccttcatg tagaaggcgc gctcggggat gtcgtcgtgc ttgccctcga
    10561 cgatctcctt gaagccgcgg atcgtgtcct cgaccttcac gtacgtcccc ggcgagccgg
    10621 tgaacgcctc cgcgacgaag aacggctgcg acaggaagcg ctcgatcttg cgcgcgcgct
    10681 gcaccgtctg cttgtcctcg tcggagagct cgtcgatgcc gaggatcgcg atgatgtcct
    10741 ggagctcctt gtaacgctgg agcacctcct tgacctcggt cgcgacctgg tagtgctcct
    10801 cgcccacgat gtcggccttc aaaatcgtgg aggtcgagtc gagcgggtcc accgccgggt
    10861 agatcccctt ctcggcgatc gaccgcgaca gcaccgtcgt ggcgttcagg tgggcgaaca
    10921 ccgacgcggg cgccgggtcc gtgaggtcgt cggccggcac gtagatcgcc tgcaccgagg
    10981 tgaccgagcc gcgccgcgtc gaggtgatcc gctcctggag ctggcccatc tcggtctcga
    11041 gcgtcggctg gtagcccacc tgcgagggca tccgcccgag cagcgccgac acctccgagc
    11101 ccgcctgcac gaaccggaag atgttgtcga tgaagagcag cacgtcctgg ccgccctcct
    11161 cgcggaagta ctcggccatc gtgaggcccg acagcgccac gcgcatgcgc gcgcccgggg
    11221 gctcgttcat ctgaccgaac acgagcatgg tcttgtcgat cacgcccgat tccttcatct
    11281 ccagccaaag gtcgttgccc tcgcgcgagc gctcgcccac gccgcagaac gcgctcaacc
    11341 cgccgtgctc ggaggcgatg ttgttgatca gctcctggat cagcacggtc ttgcccacgc
    11401 ccgcgccgcc gaacaggccg accttgccgc ccttcgcgta cggggccagc aggtccacga
    11461 ccttgatgcc ggtctcgaag atctcgcgcg tcggcgtgag gtcctccacc gtcggaaccg
    11521 ggcggtggat cggccagcgc tcggcgtcgt cctcgatctc gtcgccctcg tcgatcggct
    11581 cgccgagaag gttgaagatg cgcccgagcg tgacctcgcc cacgggcacg ctgatcggct
    11641 tgccggtgtt cctcacctcg gtgccgcggg agagccc
//
LOCUS       DASVPL010000133        24951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_173114, whole genome shotgun sequence.
ACCESSION   DASVPL010000133 DASVPL010000000
VERSION     DASVPL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24951
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173114"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VF517_06860"
     CDS             complement(<1..744)
                     /locus_tag="VF517_06860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052695.1"
                     /translation="MSIPPPGPDSVALVTGASSGIGDQFARQLAKRGFRVALVARRVD
                     RLERLVSELGGPDRAFAIPADLAVPEDRDRLVAELEERGVTVEMLVNNAGFGIYTSFV
                     DSDREQELKQVRVDVEAVVDLMARYVRGMVERERGAIINVSSTSAFQPLPYSAGYAAA
                     KAHVLLLSEAVHEEVKQSGVTVTAVCPGPVRTEFQEVNEAEFADGLPNLAWTTAERVA
                     SDALAAADKGRRSVIPGSLATRAAFAPNQV"
     gene            complement(779..1378)
                     /locus_tag="VF517_06865"
     CDS             complement(779..1378)
                     /locus_tag="VF517_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8052696.1"
                     /translation="MRKGEPLDPAATRVKVLRTAGRLFRERGTHTVGVNEIAEAAGVS
                     KLTLYRHFDSKEGLIQAFLESASDLMMRRFERTATELGLSPEERILMVMDDERRRSPG
                     RYDGCPLVNTAVEWRGSESSPGTIARVHLDRIQALLERLCDEAGFADPAAVASQLLLL
                     LEGAIVVRLVGDREDSERVARDAASALLASAPRVSRGAA"
     gene            1617..3095
                     /locus_tag="VF517_06870"
     CDS             1617..3095
                     /locus_tag="VF517_06870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8052697.1"
                     /translation="MSTEPVDAPGRADGSPQAPEGSEATSDRARWRAFLIVAPAIFIT
                     ALDMFIVNIAFPDILRDFEGSSISALSWVLNAYTIVFAALLVPMGKLGDVIGRKLVFQ
                     VGTLLFVAGSVLCATAPSPGFLIGARVLQGVGAAAITPNSLGLVLPLFPAHQRTAAIG
                     AWAALGGVGAAMGPPLGGLLTEASWRWIFVVNVPIGLAIVVLVYRRLSEVRDARALLP
                     DAIGSALAVTSVGLFVAGLVQGDTWDWDTRVLACFAAALVLGTAFVTRSLHHRAPVIE
                     LDLFKSSEFSLANVANLVFYAGFGAFLLGCVLFLTQVWHYSVIKAGFALTPPPTTAAI
                     FAGISSRLANRLGPASVGAPGGIIFAVACLMLTGLGPEPDYVGEFLPAGFVAGVGAGL
                     LIPAFMASAMLVVPPERFATGIGGSAVFRQIGAAFGVAAFVALFGTPAIPEVLDAFDR
                     TYVYMAACGAAASLMLFVLAMLMRARRPVTAEIVPATGTPAA"
     gene            complement(3159..4619)
                     /locus_tag="VF517_06875"
     CDS             complement(3159..4619)
                     /locus_tag="VF517_06875"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX8052698.1"
                     /translation="MGTSTEAPARGEAEALTPLERLETLCDPGSVRVIRSVVRSGVLG
                     SKAQEGDGVVGASGAIAGRPIFCYAEDGRFAGGSLGKAHADTIVRILELAGDARAPVV
                     GFVESAGARVQEGAAALGGYANVFRENVRLSGRMPQISIVTGSSAGGGCYSPALTDFV
                     VMTKSTSMFLTGPGVVRAVTGEDITAAELGGARVHARNGVCQFVADTDTDAAVLARVL
                     LSYLPSNAWERAPEIAATPPVTGDPGAFVPRQASLVYDVREVARRIVDGGSLLEVSPR
                     WARNMVTAFARLGGKSVGVIANQPRYIGGVIDAAGAEKGARFVRTCNAYGLPLVVLVD
                     TPGFMPGTRQESAGIIRRGAKLLYAFIEAEVPKVTVVVRQAYGGGFITMNSKELGADF
                     SFAWPQATIGIMGARQAVGITHRREIAAADDSQTALDRLAEQYVAEHQGAPAAARDGV
                     VDELVAPAETRGRLIAALDALRAKRRRQSGLGNIPL"
     gene            complement(4645..4824)
                     /locus_tag="VF517_06880"
     CDS             complement(4645..4824)
                     /locus_tag="VF517_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052699.1"
                     /translation="MDDYRNEKTTEVPPQDERENDEQRPPKYDNAEDEGTIADGGHSA
                     SAFGETGSKGERVDG"
     gene            5079..6692
                     /locus_tag="VF517_06885"
     CDS             5079..6692
                     /locus_tag="VF517_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8052700.1"
                     /translation="MSDPTAISVSRAGVGIPRPWLFWLGFAAIAAGVAVHVPMFLDAS
                     EMDYMLKGMGFDAQMIVAMSAIMIGLVLTGIGLVPRRRPDTARIPSIDVSVEEGKVSV
                     AHAALLAVLTVAVVIDAMKPAALAFVAPGMREEYGLGKNTIGLLPLMGASGMVLGALI
                     WGHFGDQVGRRAAILYSGVLFTATAVCGAMPYYELNLFMCFFMGVGVGGMIPITFALL
                     AETIPARHRGFAMVMVGVAVAGAYAVTSALSDWLIPVLSWRILFLVGLPTGMVLILLN
                     RWIPESPRFLVGMGHRQEAEAVLRRFGAELRVKPAAEGAAAAVAGRWRDIVSREYFGL
                     TLAICLVGFSAGFLTFGFQLWVPSSLTDLGIAKGDVDGLIRNAAFIAIPLSAAVAPAY
                     GYWSSKGTIIVVSLLVLTALVVLIVGGEDVASSRAALYALIALPIAGASSLIMIVAAY
                     SSEVFPTEIRARGGGAATSASRAGGVLIVLSVVFSIVSPSMEAMALAAAGALVLALAV
                     FIPQGIETRQRSLETITREELGAETAAVG"
     gene            6761..6937
                     /locus_tag="VF517_06890"
     CDS             6761..6937
                     /locus_tag="VF517_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX8052701.1"
                     /translation="MTTETHQLWICEECGFIYDPDEGDPDGGIPPGTAFDDIPADWIC
                     PACGARKDDFIPYE"
     gene            complement(6954..7289)
                     /locus_tag="VF517_06895"
     CDS             complement(6954..7289)
                     /locus_tag="VF517_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052702.1"
                     /translation="MLVVERALIDSRASGLAVLLSHVADLRELRDVVFERPVAPGDTL
                     RLSVKVGSAEPLADDGELLTTSWKVLNGAGETVVRAHVDVVCRGPWKDAAATTAAALD
                     DVDIGQIPV"
     gene            complement(7300..7941)
                     /locus_tag="VF517_06900"
     CDS             complement(7300..7941)
                     /locus_tag="VF517_06900"
                     /inference="COORDINATES: protein motif:HMM:NF038070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmbU family transcriptional regulator"
                     /protein_id="HEX8052703.1"
                     /translation="MSGEHPATGSDVTSQLCGSTEKQNGELTVAASITPTAYVIDGDV
                     PFAEWVQHGRRLGVIGRSAGWWIGDWLSYGNTRYGDRYSRASRITGYDAQTLMNMVYV
                     TSRFEPSRRHPGLSWSHHAEVAALPQEEQDRWLARAETDRLSVRCLREEIRRERRAIA
                     AREQGQLPETTGEPEPVSADYDHCPTCGNTLSVVRPRDGTAATAPARVAAASR"
     gene            complement(8019..8831)
                     /locus_tag="VF517_06905"
     CDS             complement(8019..8831)
                     /locus_tag="VF517_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8052704.1"
                     /translation="MIESTVSLLHHDPDLGRDLPPAELLAARRALPALSVTLEPGTWD
                     WASTSGRDRPPDATMLVLDGLLLLDVELASCPSAELIGPGDVLPSLDRRRNAETLLPM
                     GVELAVLEPTRVALLDESFHLACARWPSVMMALLERMEQRSWRLTTQAAICHLPAVHL
                     RLLALFWHLADRWGKVGPDHLVLPLRLLHRTLGKMVGAERPTVSLALRQLSDGGLVMR
                     RPDGAWLLRRDPSEGLELLTRCNPRPASLITDSGRDGHGVWRPDAARVSNQR"
     gene            9313..11040
                     /locus_tag="VF517_06910"
     CDS             9313..11040
                     /locus_tag="VF517_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HEX8052705.1"
                     /translation="MNGKPVPLDFPPPVDSPREFVKTSLDAEDERIEIGVVIVGGGTA
                     GLACANRLLQRLADDPQLMESLGEVPVAVIEKAKVCGGHSLSGALMRPGPLQELFPDL
                     DREDWRKEGFAFGEIEKESVYVLPNGRTKVRIPVPPPQRNHGNEVVSVSAMCRYMQKQ
                     AEEAGAYVLTETSAQQLIVEDGAVKGVRSGDKGRGKDGEPLGNFEPGTDIAARATVLA
                     EGCWGHLTGAAIRQFDLAEGREPQVWELGVKEVWKVQKPLDRVIHTLAGWPLKIPSKY
                     HQIGGSWMYPMKDEKTGDDLVSIGFIVDLDYADATTSPHDILQLFKTHPLVRNVLDGG
                     ERVSWGAKAIPAGGYWAMPKLSMPGAVIVGDAAGMVNLAALKGVHYAIQSGIIAADSI
                     YDSLKKEAGDFESYAHAVEESIIGKDLYEQRNTRQPFQMGLLRGGPLVNLMIATKGRF
                     PGGRWHLHRNDAQPMFIGNSKDRYPKPDGKYLFDKLSSVFITGNATRDDAPNHIRVQK
                     KVPREIAETWQWMCPAGVYEIPEDAPEEGEVDVIVNYTNCVQCGAITAKGGRLTSPEG
                     GDGPLYRNT"
     gene            11215..12009
                     /locus_tag="VF517_06915"
     CDS             11215..12009
                     /locus_tag="VF517_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8052706.1"
                     /translation="MDQFLSELTHDDEETQALFGRHIHWGYWADPDRADGSYQDYARA
                     AEAMSRLVCDAAKVRDGMRVLDCGCGVGGTIAALNERFSGMHLVGLNIDARQLEIARH
                     KVRAAAGNHVEFVEADACALPFEMDSFDAVLAVECIFHFKSRRRFLREVHRVLERGGR
                     LGVSDVVPQTAALPILAPLNWSLPFWGERNPVLQTLATYRALARRCGFRLSYSEDVTR
                     NTLPAYAVINSYLAGVSPEFERQGRLVGRVTRLGFLRYKILSFESR"
     gene            complement(11957..14701)
                     /locus_tag="VF517_06920"
     CDS             complement(11957..14701)
                     /locus_tag="VF517_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX8052707.1"
                     /translation="MLGPEEAARAGADRIGAKAASLVALQRLDAPVPPFACVTTAVWD
                     ELIARLAEELVAELERPPQSAERLASAGARARELIAGAELPQDDAARLAVAARAVAGP
                     GGALSVRSSAQGEDSARHSFAGQFDTYLWVSAEEVADRVLRCFASAFTDRALLYRRAH
                     GLGLDTARMAVIVQRMIESVASGVAFTADPIDTGRDGMLVVAGLGLGEGVVEGTVATD
                     TFRLDRTAETVEREIAVKSTRVVRDPSGHGTVVEDVDADEARRPALIDEQVREVARLA
                     LHVAERRGAPQDVEWALDPGGVVRLLQARPITTGAAGEETIFDNRNLVESFPGVTSPL
                     TFSLMRKTYEHNLIGLVEDFGLPRAEVERSGDVYENLVGRLSGQMFYNLSNWYRMFLL
                     IPGIERAVPAFERAMGFEPSDPASERRLSVLERMRWLPAQARVATRVLGVFVRLGSRV
                     RDFHATHARLAAEHRDLALDSMEAHELLARVERINRELFWRMSAAPINDFFTQQLYGA
                     LGAMIEAWDLGDPDATRNELLCGETGMESIEPVRSLVSLAERIAADGPATALFASPAS
                     APEVLAELEREPRLAGIRDGLRLHVERFGHRTLGELKLENETLHDDPAPVVEILRNLI
                     RAGRTVDGMERHELEIRRRAEADVRRRLRGRGWLRRALFRLVLARARFGLKARESMRL
                     TRGSIVAMLRDCYRALGERFVEAGLLERAGDVFMLTVDEVGDAVRGAAVTEDLAALAA
                     LRRAEQEAAAGRVLPPRMRTRGIALASAAVDDSPAEEGEGSLTGIGCAPGRVRARAMV
                     VEEPRADLRVDGEILVARTTDPGWVFLMVPAGGLVSEQGNVLSHTAIVGRELGIPTVV
                     GVRDAMRLLAGGVEVELDGRAGTVAICGARRAPAAREDSARASDSRRTGSCNAGNRAE
                     "
     gene            complement(14761..15783)
                     /locus_tag="VF517_06925"
     CDS             complement(14761..15783)
                     /locus_tag="VF517_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052708.1"
                     /translation="MARGGMSGAARATVAGAAAASAAMELTPAAPLRRRMAAWANERF
                     PLAGTAAMLLSLYFAALVYGRALAHEGAIEVSSGDLGAFFAVWGFFLMIRVLDEHKDY
                     ERDRVAHPDRVLQRGLVTLGQLRVLGAAAVAVQLAVSLLADGGVGAVTLWWLALIGFV
                     ALTAREFFVGEWLEGRLLLYVLAHLPLWSLAVTWMVQMGARPSSLPASAAWAAVLGFL
                     LPWGADLARRIAPSELQGPGATSYADSLGAGRAAALLGGTLIGGGAAAAAMLRAAGEH
                     SAVPVIFLTALLVPAVAALWRFRRDPHGRSANRMLQATARAVLLAELLVVVVALLADR
                     GLRWAA"
     gene            complement(15765..16781)
                     /locus_tag="VF517_06930"
     CDS             complement(15765..16781)
                     /locus_tag="VF517_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052709.1"
                     /translation="MTDARILVTGSTGLVGSELLDVLRGRGCTRVVGVSRHGGDGGLA
                     WDIGAEPPPSELQGEWDVVVNSAADTRWTLAPDDALRANVATVEALAALGVRAAHVVH
                     VSTAYAARPDGSSPGVELEDYRNTYEWSKALAERRARELFPQLTIVRPPLIMGRRSDG
                     RAARFMGMYTVIRGISSSLVPAVVGRPDALFDVIPVDDLAHAIAALAEEPPPAEPRVV
                     TVAGGASAPTVDTAVTSIVDTLNEWRTGRGLDPVAKPRLVAPASWNRFFLPFARKHLT
                     DRQLKVVDLLSNYEPYLQLDEPLAATTIVPDAAACIPASVRYWADEHSRIASLTAKPW
                     RAAA"
     gene            complement(16778..18550)
                     /locus_tag="VF517_06935"
     CDS             complement(16778..18550)
                     /locus_tag="VF517_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX8052710.1"
                     /translation="MSRATDFRTFAEALAARAEDAPDALAFTFLDEDGVEREAMTYGA
                     LRARSAAIAGMLEERLAVDERALLLLPPGLDYVAALFACFQAGVVAVSAPPPHPRQLE
                     RTLPRLLAIGADAAVGAVLTTGELTAPPPGLVPEGHALARAPWLAVDACTSDGDATAS
                     ARAREPADLAILQYTSGSTADPRGVMLTHANLLVNSELIAQRLGSSGADRGFIWLPPY
                     HDMGLIGGILQPVYRGFECVLTSPLTVVKRPERWLEGISTRRATISGGPNFAFDLCVR
                     RIGPEVRERLDLSSWEVAFNGAEPIRAGTLEAFATAFAPCGFHREAFLPCYGLAEATL
                     MVTAGERDEPPALRRFDARALERGRAEPVAGAGLELVGCGGPDPDHELAIVDPETLMR
                     CEPGRIGEIWVAGASVARGYLGHGDENARAFGAQVAGEANGERFLRTGDLGFTSDGEL
                     FVVGRIKDLLVVNGRNLHPHDVEVVAEAADERLRPHSSAAFALGDGTLGDERVAFVAE
                     LTDASAASAAIDALRREVGTQLGLDLELVVLCQRGSVPKTTSGKVQRRLCRSLLLRGE
                     LNVIASQGAEPGLGAAPERAATDR"
     gene            complement(18547..18822)
                     /locus_tag="VF517_06940"
     CDS             complement(18547..18822)
                     /locus_tag="VF517_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX8052711.1"
                     /translation="MSGSPAAADATLAARVAALVLELSPRRATAAPEDALLVTDLGYE
                     SLALIELAAALEDEFRIGPVAADDALAVETIADVTRLVAGFFEGGGS"
     gene            complement(18819..19583)
                     /locus_tag="VF517_06945"
     CDS             complement(18819..19583)
                     /locus_tag="VF517_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8052712.1"
                     /translation="MAALTMIRNDLSLMRADPTAPIILFAMPLVLVLFIEPNQAAVLE
                     QHGYANANGAEHAVPGMGVLFAFFVTSYAGIAFFREHVWGTWDRIRALPVRPRDVVLG
                     KTALWFLLLCAQQLVLFLVGVLLLGVDVRGSVVALVAVALSFVLWVEALILLTIAYAS
                     TLQQVLAVANLSAILFAALGGALTPSAALPEWAEAIAPATPSHWAMKGFEGILLDGDG
                     LADAIVPVAILTGSAMVLLLLAARRFRFDEARVGTL"
     gene            complement(19571..20554)
                     /locus_tag="VF517_06950"
     CDS             complement(19571..20554)
                     /locus_tag="VF517_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8052713.1"
                     /translation="MSAHLGSSASNGGGPMLSLSGVARSYGEVRALDGVDLEIARGEI
                     LALLGPNGAGKTTLVSIVAGLLEPDAGRVLVGGIDVQRRPLEARRLIGVAPQALGFYL
                     QLTVLANMRFFGRMAGLGRRELEDRIEQVAGLFGLADLLGRTATDLSGGEKRRLHTAL
                     ALLHRPPLLLLDEPTAGVDVHMRVELLDVVKRMASEGAAVCYTTHYLHEVELLSASAA
                     ILEYGRIVAAGRVPELIEAHGKSVVEIRFDGPAPREAAVLGPLNDGGSVVRIDTPEPT
                     AAVARAFASMGPAAASVRGVEIVRPSLETAYLSITGRRFEIAPDGTDAWQP"
     gene            20651..21310
                     /locus_tag="VF517_06955"
     CDS             20651..21310
                     /locus_tag="VF517_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX8052714.1"
                     /translation="MTTTRSVRRRLDLTRPVPRSVVLECISVATQAPSGGDRQHWRFV
                     VVDEPDLRAEIAAHYRRAHRERREEAAAGSTPGGGGNPRLERSVDDLAERLASVPVLV
                     IPCVLAPPSPTTLAAQASLWGSILPATWSFMLAARLHGLGTTFTTLHLRYADEVAALL
                     GIPAGATQAGLVPVAYARGTRFRPARRRPVEQVVHWNGWAGAAASPHPSPRRRSSDTI
                     C"
     gene            21720..22526
                     /locus_tag="VF517_06960"
     CDS             21720..22526
                     /locus_tag="VF517_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX8052715.1"
                     /translation="MLVKPDAVAGRRLDATLRWLAEHEVAIVAAERCRLTRRTIRALW
                     QYQWNLATRDRRDLADLLMPSVESLLLVLRPRAGQGRDDVPFTLTLNRMKGPADPEAR
                     APGQLRHSLGNENYLLNFVHTTDEPADMVREIGVLFEPAVRRRVYEAARDGRDAAQDA
                     RREVDELHRQVAPQDLSLAGALERVAGRARALQPSRERDELLAAVERVRSGDERDWRG
                     LRALADRADVPLERWDQIALGTHLMNAKEEGREPVLAGVTAADWLAAHRE"
     gene            22529..23530
                     /locus_tag="VF517_06965"
     CDS             22529..23530
                     /locus_tag="VF517_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="HEX8052716.1"
                     /translation="MTTGTVTTDRASELELSSEHPIPREVVHKLAVAEVFVTDSARLS
                     DDSMLLAGELPRSHYYYSDGGSFPARFDVMPILELCRQACFVMSHRQLGVPIGQQFIL
                     RSLGVELDPAAIFQRQEEPLRVTVRCDVLRRWTDGDRLVGALWRFTLRTPRELPVATA
                     TAAMSWMPKERWAEVRAGNRTQRGLPPEIAYARPGWSNVPPASVGRECRENVVVSDIQ
                     LRDGGVRATLIADILHPGLFDHYVDHLSGMLEAEACRQLALCCAGGGLPVSRDVAVSA
                     IDVAFAAVGELDLPTLCTAAVQPVDGACDTRRVDAELTQQGDPLCRATLTVSTGPDA"
     gene            23527..24270
                     /locus_tag="VF517_06970"
     CDS             23527..24270
                     /locus_tag="VF517_06970"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX8052717.1"
                     /translation="MTGHFQIADRVLASRLMLGTGGFRSLETLARCLRAGPVEICTVA
                     LRRVGSTPRQPVLQVLDAAGVSVLPNTAGCHTARDAVVTARLAREAFETDWIKLEIVG
                     DRRTLMPDARELLDAAATLAGEGFVVLPYSTDDPVLARRLEDAGCAAICPGGSPIGSG
                     MGLNNPSNLALLCENAGVPVVLDAGIGTASDAALAIELGCDAVMTATGISRARDPVGM
                     ARALHGAVQAGYLARVGERVPRLPAGAAA"
     gene            24267..>24951
                     /locus_tag="VF517_06975"
     CDS             24267..>24951
                     /locus_tag="VF517_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8052718.1"
                     /translation="MTARGGSAGTTASEPAVLERRLGDYHDVTMAEVLGIVGYLRDDG
                     DSVIVGGSLAIGLGNGLSDLDVVVAGRETPASQVPLEHWVKSLRVDVWLRSQRDVDAL
                     FERAESALESERPLQGAFGSVDEEQELKLLHRVAFGLHWDGPELVPSTRDYPDIARDL
                     LVREYAERMRESACVATLAHAAGRDRAAAVNARLAVEEAMQAVLAARGHPWSGDKWLQ
                     VRLQEHEGEC"
BASE COUNT         3511 a         9014 c         8919 g         3507 t
ORIGIN      
        1 gacctggttg ggcgcgaacg cggcccgtgt ggcgaggctg ccggggatca cggagcggcg
       61 gcccttgtcc gccgccgcga gcgcgtccga cgccacacgc tcggccgtgg tccaggccaa
      121 gttcggaagg ccgtcggcga actcggcctc gttgacctcc tggaactcgg tgcgcaccgg
      181 gccgggacag accgcggtga ccgtgacccc gctctgcttg acctcctcgt gcacggcctc
      241 ggagagcagc aggacgtgcg ccttggccgc cgcgtacccc gcgctgtagg gcagcggctg
      301 gaacgcggac gtcgacgaga cgttgatgat cgcgccgcgt tcgcgctcga ccatcccccg
      361 cacgtaacgg gccatcaggt cgacgaccgc ctccacgtcg acgcgcacct gcttcagctc
      421 ctgctcgcgg tcggagtcga cgaacgacgt gtagatcccg aagccggcgt tgttgaccag
      481 catctcgacc gtcacgccgc gctcctccag ctccgcgacg agcctgtccc gatcctccgg
      541 gacggcgagg tcggccggga tcgcgaacgc gcggtccggg ccccccagct cggacacgag
      601 tcgttcgagc ctgtccactc gccgcgcgac gagggcgacg cgaaacccgc gcttggcgag
      661 ctggcgcgcg aactggtcgc cgatgccgga ggaggcgccg gtgaccagcg cgaccgagtc
      721 ggggcccggc ggtgggatgc tcacgcgcgg gatcgtacgt cgtaccggca gccggcggtc
      781 aagcggcgcc gcggctgacc cgcggagcgc tcgcgagcag ggcgctggcc gcgtcgcgcg
      841 ctacgcgctc ggaatcctcg cgatcgccca cgaggcgcac gacgatcgcg ccctcgagga
      901 gcaggaggag ctggctcgcg acggcggcgg ggtcggcgaa cccggcttcg tcgcatagcc
      961 gctccaggag agcttggatg cggtccagat gcacgcgggc gatcgtgccg ggcgacgact
     1021 cggagccgcg ccactcaacc gcggtgttca cgagcgggca gccgtcgtag cgaccgggag
     1081 accggcgccg ttcgtcgtcc atgaccatga ggatccgctc ttcgggcgac agaccgagct
     1141 ctgtggcggt gcgctcgaac cgccgcatca tgaggtcgct cgccgactcg aggaacgcct
     1201 ggatcaggcc ctccttcgaa tcgaagtggc ggtagagggt cagcttcgag acgccggcgg
     1261 cctcggcgat ctcgttcact ccgaccgtgt gggtgccgcg ctcgcggaac agccggcccg
     1321 cggtcctcag gaccttgacg cgagtcgccg cagggtcgag cggctcaccc ttcctcaccg
     1381 gcatcgccta aaccgccttc cgtgtctgat cggaccgcac atgatgcacc gatcaccttc
     1441 gaggtgtccg atcggacgtg aagataccga ccggtatgta tcatcgcgcc gcggcagggg
     1501 cggcgacgag atggggaggc ggggatggag acggctacgg agataccgcg gcggacggag
     1561 ataccggacg ccgaggccca ggacccgtac gtgctctcgc cgcggaggga ggggcgatga
     1621 gcaccgagcc cgtggacgca ccgggtcgcg cggacggatc gccgcaagcg cccgaaggca
     1681 gcgaggcgac gagcgacagg gctcgctggc gagcgttcct gatcgtcgcg cccgcgatct
     1741 tcatcacagc cctcgacatg ttcatcgtga acatcgcgtt cccggacatc ctccgggact
     1801 tcgagggctc ctcgatatcg gcgctgtcgt gggttctcaa cgcgtacacg atcgtgttcg
     1861 cggcgctact cgtcccgatg ggcaagctcg gcgacgtgat cggtcgcaag ctcgtcttcc
     1921 aagtcgggac gctcctgttc gtggccggct ccgtgctgtg cgccaccgcg ccgtcgcccg
     1981 gcttcctgat cggcgcgcgc gtgttgcagg gcgtgggcgc cgccgccatc accccgaact
     2041 cgctcggact cgtcctccca ctcttccccg cgcaccagcg caccgcggcg atcggcgcct
     2101 gggcggcact gggcggagtc ggcgcggcca tggggccacc gctcggcggg ctgctcacgg
     2161 aggccagctg gcgctggatc ttcgtcgtca acgtcccgat cgggctcgcc atcgtcgtgc
     2221 tcgtgtaccg ccggctgagc gaggtgcgcg acgcccgcgc cctcctgccg gacgcgatcg
     2281 ggtcggcgct cgccgtcacc tcggtcgggt tgttcgtggc cgggctcgtc cagggcgaca
     2341 cgtgggactg ggacacgcgc gtgctggcgt gcttcgcggc ggcgctcgtg ctcggcaccg
     2401 ccttcgtgac gcgctcgctg caccaccgcg cgccggtcat cgagctcgac ctgttcaagt
     2461 cgtccgagtt ctcgctggcc aacgtggcga acctcgtctt ctacgccggc ttcggcgcgt
     2521 tcctgctcgg ctgcgtgctc ttcctcaccc aggtctggca ctactcggtg atcaaggccg
     2581 gcttcgccct gacgccccct ccgaccacgg ccgcgatctt cgcggggatc tcgagccggc
     2641 tcgccaaccg cctcggcccg gcgagcgtcg gcgcgcccgg cgggatcatc ttcgcggtcg
     2701 cctgcctgat gctcaccgga ctggggccgg agccggacta cgtcggcgag ttcctgccgg
     2761 cgggcttcgt cgccggcgtc ggcgccgggt tgttgatccc ggcgttcatg gcctccgcga
     2821 tgctggtcgt cccgcccgag cggttcgcga ccgggatcgg cggctcggcc gtgttccgcc
     2881 agatcggagc ggcgttcgga gtcgccgcgt tcgtggcgct gttcgggaca ccggcgatcc
     2941 ccgaggtcct ggacgcgttc gatcgcacct acgtctacat ggcggcgtgc ggagcggcgg
     3001 ccagcctgat gctcttcgtg ctggcgatgc tgatgcgggc acgccgtccg gtgacggcgg
     3061 agatcgtccc agcgaccggc acaccggccg cgtgatctcg accaccacgg ccgtccggcc
     3121 ttccgagcgc tcgaccgcag cttcgtcgta gcgcgcggct acagcgggat gttgccgagg
     3181 ccgctctgtc tgcggcgctt cgcgcgcagc gcgtcgagtg ccgcgatcag ccggccgcgt
     3241 gtctccgccg gggcgacgag ctcgtctacg acaccgtcgc gtgcggcggc cggcgcgccc
     3301 tggtgctcgg cgacgtactg ctcggcgagc cggtcgagcg ccgtctgcga gtcgtcggcc
     3361 gcggcgatct cgcggcggtg cgtgatgccg accgcctggc gcgcgcccat gatcccgatc
     3421 gtcgcctgcg gccacgcgaa cgagaagtcg gcgccgagct ccttcgagtt catcgtgatg
     3481 aagccgccgc cgtaggcctg gcggaccacg acggtgacct tcggcacctc cgcctcgatg
     3541 aacgcgtaga gcagcttcgc tccgcggcgg atgatgccgg ccgactcctg cctcgtcccc
     3601 ggcatgaagc ccggcgtgtc gaccagcacg accagcggga ggccataggc gttgcaggtg
     3661 cggacgaagc gggcgccctt ctcggccccg gccgcgtcga tcactccacc gatgtagcgc
     3721 ggctggttgg cgatcacgcc gaccgacttg ccgccgaggc gggcgaacgc ggtgaccatg
     3781 ttccgcgccc agcgcggcga gacctcgagc aggctgccgc cgtccacgat ccgcctggcg
     3841 acctcgcgca cgtcgtagac cagcgaggcc tgacgcggca cgaacgcgcc cgggtcgccg
     3901 gtgaccggcg gcgtcgcggc gatctcgggg gcccgctccc acgcgttcga cggcaggtac
     3961 gagagcagca cccgggcgag gacggccgcg tcggtgtcgg tgtcggccac gaactggcac
     4021 acgccgttgc gggcgtgcac cctcgcgccg ccgagctcgg cggccgtgat gtcctccccg
     4081 gtcacggcgc ggaccacgcc ggggccggtc aggaacatgc tcgtggactt ggtcatcacg
     4141 acgaagtcgg tgagcgcggg cgagtagcag ccgccgccgg ccgagctgcc ggtgacgatc
     4201 gagatctgcg gcatccttcc ggacagcctg acgttctccc ggaagacgtt cgcgtacccc
     4261 ccgagcgcgg ccgctccctc ctgcacgcgc gcgcccgccg actcgacgaa tccgaccacg
     4321 ggcgcccgcg cgtcgccggc gagctcgagg atgcgcacga tcgtgtcggc gtgcgccttg
     4381 ccgagggagc cgccggcgaa gcggccgtcc tcggcgtagc agaagatggg acgcccggcg
     4441 atcgcccccg aggcgccgac gacgccgtcg ccctcctgcg ccttcgagcc gagcacgccg
     4501 gagcggacga ccgaccggat cacgcgcacc gagccggggt cgcacagcgt ctcgaggcgc
     4561 tccagcgggg tcagggcctc cgcctcgccg cgtgccggag cttcggtcga ggtacccaca
     4621 gccgggcgtt cgggcctcgc gcccctagcc gtccacccgt tcgcccttgg agccggtctc
     4681 gccgaaggcg ctggcgctgt ggccgccgtc ggcgatcgtt ccctcgtcct ccgcgttgtc
     4741 gtacttcggc gggcgctgct cgtcgttctc gcgctcgtcc tgcggcggta cctcggtcgt
     4801 cttctcgttc ctgtagtcgt ccatgcggac atgctccgtg tcagtcccgc gccggtcaaa
     4861 gcgagctcga agtcgggcgt cacgccggcc gattgccgat ctcccgacgc cgggggactc
     4921 gcggggttca gcgactcgcg gacgtacggc cttcccggcg tgaggtgaat tgtcttctca
     4981 gcgcgtaggt gaatttgaac gcttcgcgat gtcctgtgac cgtacgggcc gacaaagcac
     5041 gagctcgacc gggggggcta cgaggtgtac atcaggacat gagcgatccg accgcaatca
     5101 gcgtcagccg tgcgggggtg ggcataccgc gcccctggtt gttctggctc ggcttcgccg
     5161 cgatcgccgc cggcgtcgcg gtccacgtcc cgatgttcct cgacgccagc gagatggatt
     5221 acatgctcaa gggcatgggg ttcgacgccc agatgatcgt cgccatgtcg gcgatcatga
     5281 tcggcctcgt tctcacggga atcggcctgg tgccgcgcag gcgccccgat accgcgcgca
     5341 tcccgagcat cgacgtcagc gtcgaggagg gcaaggtcag cgtcgcgcac gcggcgctgc
     5401 tggccgtcct cacggtcgcg gtagtgatcg acgcgatgaa gccggcggcc ctggcgttcg
     5461 tcgcccccgg catgagggag gagtacggac tcggcaagaa caccatcggg ctgctgccgc
     5521 tgatgggcgc cagcgggatg gtgctcgggg ccctgatctg gggtcacttc ggcgaccagg
     5581 tcgggcggcg cgcggcgatc ctctactcgg gcgtgctctt cacggcgacg gccgtctgcg
     5641 gcgccatgcc gtactacgag ctcaacctgt tcatgtgctt cttcatgggc gtcggcgtgg
     5701 gcgggatgat cccgatcacc ttcgcgctgc tcgccgagac catcccggcg cggcaccgcg
     5761 ggttcgcgat ggtgatggtc ggtgtggcgg tcgcgggcgc ctacgcagtg acgagcgctc
     5821 tgtcggactg gctcatcccg gtgttgagct ggcgcatcct gttcctggtc gggctgccca
     5881 cgggcatggt gctgatcctc ctgaaccgct ggatcccgga gtcgccgcga ttcctcgtgg
     5941 gcatgggcca caggcaggag gcggaggccg tgctgaggcg cttcggcgcc gagctccggg
     6001 tgaagcccgc ggccgaggga gctgcggccg cggtcgccgg acgctggcgg gacatcgtct
     6061 cccgcgagta cttcggcctg acgctggcca tctgcctggt gggcttcagc gccgggttcc
     6121 tcacctttgg attccagctc tgggtgccct cgagcctgac cgacctcggc atcgcaaagg
     6181 gcgacgtgga cgggctgatc cgcaacgcgg cgttcatcgc gatccccctg agcgccgcgg
     6241 tcgccccggc gtacgggtac tggagcagca agggcacgat catcgtcgtc tcgctgctcg
     6301 tcctgaccgc gctcgtcgtg ctgatcgtgg gcggcgagga cgtcgcgtcg agccgggccg
     6361 ccctctatgc gctgatcgcg ctgccgatcg ccggcgcaag ctcgctcatc atgatcgtcg
     6421 ccgcctacag ctcggaggtg ttccccaccg agatacgcgc gcgagggggc ggcgcggcca
     6481 cgagcgccag ccgtgccggg ggagtgctga tcgtcctctc ggtcgtgttc tccatcgtca
     6541 gcccgtccat ggaggcgatg gccctggccg cggccggagc gctcgtcctc gcgctggcgg
     6601 tgttcatccc ccagggcatc gagaccaggc agcgctcgct cgagacgatt acgcgcgagg
     6661 agctcggcgc cgagaccgcg gcggttggct agccggggcg gatgtggatg tgcccgcgac
     6721 ggttttctcg cgcccgggcg atcggcgtaa tctgaccacc gtgacgaccg agactcacca
     6781 gctctggatc tgcgaggagt gcgggttcat ctacgacccc gacgagggcg atcccgacgg
     6841 cgggatcccg cccggcacgg cgttcgacga catccccgcc gactggatct gtccggcctg
     6901 cggcgcgcgc aaggacgact tcatcccgta cgagtagccg cgggatccgg cggctagacc
     6961 gggatctgcc cgatgtcgac gtcgtcgagc gccgcggcgg tcgtggccgc ggcgtccttc
     7021 cagggcccgc ggcacaccac gtcgacgtgc gcgcgcacga cggtttcccc agctccgttg
     7081 aggaccttcc acgaggtggt gagaagctcg ccgtcgtccg ccagcggctc ggcggagccg
     7141 accttcacgc tcaatcggag ggtgtcgccg ggcgccaccg ggcgctcgaa cacgacatcg
     7201 cgcagctcgc ggaggtcggc gacatgtgaa agcagtacgg cgaggccgct cgcgcggctg
     7261 tcgatcagcg cgcgctcgac gacgagcatc gaaccctcgt cagcggctcg ctgcggccac
     7321 ccgagccggc gcggtcgcgg cagtgccgtc cctgggccgc acaaccgaga gcgtgttgcc
     7381 gcacgtcggg cagtggtcgt agtccgccga caccggttcg ggctccccgg tggtttcggg
     7441 cagctggccc tgctcgcgcg ccgcgatcgc ccgccgctcg cgccggatct cctcgcgcag
     7501 gcagcgcacc gacaggcggt cggtctcggc acgcgccagc cagcggtcct gctcctcctg
     7561 cggcagcgcg gccacctcgg cgtgatggct ccacgacagg cccgggtggc gccgggacgg
     7621 ctcgaagcga gaagtcacgt acaccatgtt catgagcgtc tgcgcgtcgt agccggtgat
     7681 gcgcgacgct cgcgagtagc ggtcgccgta cctcgtgttc ccgtagctca gccagtcgcc
     7741 gatccaccac ccggcgctac gcccgatgac tccgagtcgc cggccgtgtt gaacccactc
     7801 ggcgaacggc acgtctccgt cgatcacgta cgcggtgggg gtgatcgacg cggcgacggt
     7861 gagttcgccg ttctgcttct cagtcgatcc gcagagctgt gaagttacgt ctgaaccggt
     7921 agctggatgc tcgccgctca cttattcccc tcggtggtta cacgtcgaaa ccggttgata
     7981 ccacccgaat gtgggatcga cacagcggtg gtttgtagtc aacgttggtt actaacccgc
     8041 gcggcgtccg gccgccaaac gccgtgaccg tcgcgacctg agtccgtgat gagtgaggcg
     8101 ggccgggggt tacaacgcgt caggagctcc aggccctcgc tcggatcgcg gcgcaggagc
     8161 caggcaccgt cgggccgccg catcaccagg ccgccgtcgg acagctgccg gagcgcgagg
     8221 ctcaccgtcg ggcgctcggc cccgaccatc ttccccaggg tgcggtgaag gaggcgaagc
     8281 ggaagcacca gatggtccgg tccgaccttc ccccagcggt ccgcaagatg ccagaagagt
     8341 gcgagcagcc gcaagtgcac cgccggcagg tgacagatag ccgcctgtgt ggtgagccgc
     8401 caggagcgct gctccatccg ctcgagcagc gccatcatga ccgacggcca acgcgcgcac
     8461 gcaagatgga aactctcgtc gagcagggcg acgcgcgtcg gctcgaggac tgcgagctcg
     8521 acgcccatgg gcaggagcgt ctccgcgttc cggcgccggt ccagcgacgg taggacgtcg
     8581 cccggtccga tcagctcggc ggaggggcag ctcgccagct cgacgtccag caggaggagc
     8641 ccgtccaaaa ccagcatggt tgcgtcgggc ggacggtcgc ggccgctcgt gctcgcccag
     8701 tcccaggtgc cgggctcgag cgtgaccgac agcgcgggca gcgccctccg cgcggccagg
     8761 agctctgcgg gcggaagatc ccgcccgagg tccggatcat ggtggagcag tgaaacggtc
     8821 gattcgatca tcgcgccgtg agcccctcct ttcaccgcgc gccccgccgt tggtttcagg
     8881 accttgagcg gttaacttac cgttataacg gcgcccaaac gggcgccacg tggggggtgt
     8941 tcgtaacccc tgatcagggg ggtgcggtgc aacgcccgtc cggcgtcaac ctcggatggc
     9001 tgttcacggt ccccgcgagc agctctgagg tcgacacggc gacccgaacg gagaagttgt
     9061 atgtggtctg tactcatgct tccagtgggg tcatgcgcac cgatgaaaac gcttcaagca
     9121 cctcgcccac cgttggcctg gcgaagcgtg cgcgcgctcc gcaagatgag cgttcgctcc
     9181 tcgtgccggc gacgtgaccg gcggcccgtc gcgcgtggca cgaccggcgg tgggaggagg
     9241 atccctacca ttgtcgcgcg cgccccggcg ccctgatctc gctcccgacc cgttaactgc
     9301 gcaatgacct ccatgaacgg caagcccgtc ccgctcgact tcccaccgcc ggtcgactcg
     9361 ccgcgcgagt tcgtcaagac ctcgctcgac gccgaggacg agcggatcga gatcggcgta
     9421 gtcatcgtcg gcggcggcac cgcgggcctc gcgtgcgcga accgtctgct ccagcgtctc
     9481 gccgacgacc cgcagctgat ggagagcctc ggcgaggtgc cggtggcggt catcgagaag
     9541 gccaaggtct gcggcggcca cagcctgtcc ggggccctga tgcggccggg cccgctgcag
     9601 gagctgttcc cggacctcga tcgcgaggac tggcgcaagg agggattcgc gttcggcgag
     9661 atcgagaagg agtcggtcta cgtcctgccc aacggcagga cgaaggtccg gatcccggtc
     9721 ccgccgccgc agcgcaacca cggcaacgag gtcgtttcgg tgtccgcgat gtgccgctac
     9781 atgcagaagc aggcggagga ggcgggcgcc tacgtcctca cggagacctc ggcgcagcag
     9841 ctgatcgtcg aggacggggc ggtcaagggc gtccggtcgg gcgacaaggg ccgcggcaag
     9901 gacggcgagc cgctcggcaa cttcgagccc ggcaccgaca tcgccgcgcg cgcgacggtg
     9961 ctcgcggagg gctgctgggg tcacctcacc ggggccgcga tccgccagtt cgacctcgcc
    10021 gaggggcgtg agccgcaggt gtgggagctc ggcgtgaagg aggtctggaa ggtccagaag
    10081 ccgctcgacc gcgtgatcca cacgctcgcc ggctggccgc tgaagatccc gtccaagtac
    10141 caccagatcg gtggttcgtg gatgtacccg atgaaggacg agaagaccgg cgacgacctg
    10201 gtctcgatcg ggttcatcgt ggacctcgac tacgccgacg cgacgacgtc gccgcacgac
    10261 atcctccagc tgttcaagac gcacccgctc gtgcgcaatg tcctcgacgg cggcgagcgt
    10321 gtgtcgtggg gcgcgaaggc gatccccgcg ggcggctact gggcgatgcc gaagctgtcg
    10381 atgccgggag cggtgatcgt cggcgacgct gccggcatgg tcaacctcgc cgcgctgaag
    10441 ggcgtccact acgcgatcca gtcgggcatc atcgccgccg actcgatcta cgactcgctg
    10501 aagaaggagg ccggcgactt cgagtcgtac gcgcacgccg tcgaggaatc gatcatcggc
    10561 aaggacctct acgagcagcg caacacgcgg cagccgttcc agatgggcct gctgcgcgga
    10621 ggtccgctgg tcaacctgat gatcgccacg aagggccgct ttccgggcgg ccgctggcac
    10681 ctgcaccgca acgacgcgca gccgatgttc atcggcaact cgaaggaccg ctacccgaag
    10741 ccggacggca agtacctatt cgacaagctc tcgagcgtgt tcatcaccgg caacgccacg
    10801 cgcgacgacg cgccgaacca catccgggtg cagaagaagg tgccgcgcga gatcgccgag
    10861 acctggcagt ggatgtgtcc cgcgggcgtc tacgagatcc ccgaggacgc gcccgaggag
    10921 ggcgaggtcg acgtgatcgt gaactacacg aactgcgtgc agtgcggcgc gatcacggcc
    10981 aagggcggcc ggctgacgtc gcccgagggc ggcgacgggc cgctgtaccg aaacacctag
    11041 cccgcggccc ggggagccct tcgtcccgag acttctggag cgctcagatc gggttatgct
    11101 ccagagcttc tcgccatgcc ttagccgtga agatggcgat ggcaggtgaa gggagcagca
    11161 gtgagtcgac gatccacgac cgagccggtc gcgcgcacgc cgcggatgcc gtacatggat
    11221 caattcctga gcgagttgac tcacgacgac gaggagacgc aggcgctctt cggccggcac
    11281 attcattggg gctactgggc ggacccggac cgtgcggacg gctcctacca agactacgcc
    11341 cgcgcggccg aggcgatgtc ccgtctggtc tgcgacgccg cgaaggttcg cgacggcatg
    11401 cgagtgctcg actgcggctg tggcgtgggc ggcacgatcg ccgccctgaa cgagcgcttc
    11461 tccggaatgc acctcgtcgg cctgaacatc gacgcgcgcc agctcgagat cgccaggcac
    11521 aaggtcaggg ccgctgccgg caatcacgtc gagttcgtcg aggccgacgc ctgcgcgctg
    11581 cccttcgaga tggattcctt cgacgccgtg ctcgctgtcg agtgcatctt tcacttcaag
    11641 agccgtaggc gcttcctgcg cgaggtgcat cgcgtcctgg agcggggagg gcgcctgggg
    11701 gtgtccgacg tcgtcccgca gacggcggcg ctgccgatcc tcgctcccct gaactggtcc
    11761 ttgccgttct ggggtgagcg caacccggtt ctgcagacgc tggccaccta ccgagcgttg
    11821 gctcggcggt gcggtttccg gttgtcctac agcgaggacg tcacacggaa caccctcccc
    11881 gcgtatgccg tgatcaatag ttatctcgcc ggtgtttcac cggagttcga gcgtcaagga
    11941 cgcctcgtcg ggcgcgtcac tcggctcggt ttcctgcgtt acaagatcct gtccttcgag
    12001 agtcgctagc gcgcgccgag tcctctcgcg cggcgggggc gcgacgtgcg ccgcagatcg
    12061 ccacggtccc ggcccgcccg tcgagctcga cctcgacgcc gccggcgagc agccgcatgg
    12121 cgtcgcgcac tcccaccacc gtcgggatcc ccagctcgcg gccgacgatc gccgtgtgcg
    12181 agaggacgtt gccctgctcg gagacgaggc ctccggcggg gaccatcagg aagacccagc
    12241 ccggatcggt cgtccgcgcg acgaggatct ctccgtcgac gcgcaggtcg gcgcgcggct
    12301 cctcgaccac catcgctcgc gcccgcacgc ggccgggcgc gcagccgatg ccggtgaggc
    12361 tcccctcgcc ctcctccgcc ggcgagtcgt ccacggccgc gctcgcgagc gcgatcccgc
    12421 gcgtgcgcat gcgtggcggc aggaccctgc ccgccgccgc ctcctgttcc gcgcggcgga
    12481 gcgccgctag cgccgccagg tcctccgtca ccgcggcgcc gcgcaccgcg tcgcccacct
    12541 cgtccacggt cagcatgaac acgtcgccgg cgcgctcgag cagccccgcc tccacgaagc
    12601 gctcccccag cgcgcggtag cagtcgcgga gcatcgccac gatgctcccg cgcgtgagcc
    12661 gcatgctctc cctggccttc aacccgaagc gggcgcgggc gagcacgagc ctgaacagcg
    12721 cgcggcgcag ccagccccgc ccccgaaggc gccggcgcac gtccgcctcg gcacgccgcc
    12781 ggatctccag ctcgtgccgc tccatcccgt ccaccgtccg gcccgcgcgg atcaggttgc
    12841 gcaggatctc cacgaccggc gccgggtcgt cgtgaagggt ctcgttctcc agcttgagct
    12901 ctcccagcgt gcggtgcccg aagcgctcga cgtgcaggcg gaggccgtcg cgtatgcctg
    12961 caagacgggg ctcgcgctcg agctccgcga gcacctccgg agcggatgcc ggcgatgcga
    13021 acagggccgt ggcgggcccg tccgcggcta tgcgctccgc gaggctcacc agcgagcgca
    13081 cgggctcgat gctctccatt cccgtctcgc cgcagagcag ctcgttgcgc gtggcgtccg
    13141 gatcgccgag gtcccatgcc tcgatcatcg cgccgagcgc cccgtagagc tgctgcgtga
    13201 agaagtcgtt gatcggggcg gccgacatcc tccagaacag ctcgcggttg atgcgctcga
    13261 cgcgtgcgag gagctcgtgt gcctccatgc tgtcgagggc gaggtcgcgg tgctcggccg
    13321 ccagccgcgc atgggtggcg tggaaatcgc gcacgcgcga gccgagccgg acgaacaccc
    13381 cgagcactcg cgtcgcgacg cgcgcctgag cgggcaacca gcgcatccgc tcgagcacgg
    13441 agagccgccg ctccgaggcc gggtcgctcg gctcgaaccc catcgcccgc tcgaacgcgg
    13501 gcaccgctcg ctcgatcccg gggatgagca ggaacatccg gtaccagttg gagaggttgt
    13561 agaacatctg cccggacagg cgcccgacga ggttctcgta gacgtcgccg ctgcgctcga
    13621 cctcggcgcg cggcagcccg aagtcctcca cgagcccgat caggttgtgc tcgtacgtct
    13681 tcctcatgag cgagaaggtg agcggcgagg tgaccccggg gaagctctcg acgaggttgc
    13741 ggttgtcgaa gatcgtctcc tcgcccgcag ccccggtggt gatcggccgc gcctggagca
    13801 ggcgcaccac gccgccgggg tcgagcgccc actccacgtc ctgcggcgcc ccccggcgct
    13861 cggccacgtg cagcgccagg cgcgccacct cgcgcacctg ctcgtcgatc agcgccggac
    13921 ggcgcgcctc gtcggcgtcc acgtcctcga ccaccgtccc gtgcccgctc ggatcacgaa
    13981 cgacgcgtgt cgacttgacc gcgatctcgc gctcgaccgt ctccgccgtc cggtccagcc
    14041 tgaaggtgtc ggtggcgacc gtgccctcga cgacgccctc cccgagcccc aggccggcga
    14101 ccaccagcat gccgtcgcgg ccggtgtcga tcgggtcggc ggtgaaggca actccggacg
    14161 ccacgctctc gatcatccgc tggacgatca ccgccatccg ggcggtgtcg aggcccaggc
    14221 cgtgggcgcg gcggtagagc agcgcacgat cggtgaacgc ggaggcgaag cagcgcagaa
    14281 cccggtcggc cacctcctcc gcggagaccc acaggtaggt gtcgaactgg ccggcgaacg
    14341 agtgccgtgc ggagtcctcg ccctgcgcgg aggaacgcac cgagagggcg cccccgggac
    14401 ccgcgaccgc acgcgccgcg acggccagtc gcgcggcatc gtcctgcggc agctccgcgc
    14461 cggcgatcag ctcgcgtgcc ctggcgccgg cgctcgcgag ccgctccgcg ctctgcggcg
    14521 gccgctcgag ctcggcgacc agctcctcgg ccaggcgcgc gatcagctcg tcccataccg
    14581 cggtggtcac gcacgcgaac ggcggcacgg gcgcgtcgag ccgctggagc gccacgaggc
    14641 tcgccgcctt cgcgccgatc cgatcggccc cggcccgcgc cgcctcctcg gggccgagca
    14701 cgcgcggctc gagtcgaggc tcggcagtcg cggacgctgc actcgccggc gccgaactgc
    14761 tcacgcggcc cacctcagcc cgcgatcggc caggagcgcc acgaccacca cgagtagctc
    14821 ggcgagcagg acggcgcgag ccgtcgcctg gagcatccgg ttcgccgagc gtccgtgcgg
    14881 atcgcgccgg aacctccaca gcgcggcgac ggccggcacg agcagcgcgg tgaggaagat
    14941 caccgggacg gccgagtgct cccccgcggc gcgcagcatc gccgcggccg cggcgccccc
    15001 accgatcagg gtcccgccga gcagcgcggc ggcccgtccc gcgccgaggc tgtcggcgta
    15061 cgacgtggcg cccggtccct ggagctcgga gggtgcgatg cgccgcgcga ggtccgcccc
    15121 ccagggcagc aggaacccga gcacggccgc ccacgccgcg ctcgccggca gcgagctcgg
    15181 ccgggcgccc atctgcacca tccaggtgac cgccagggac cagagcggaa ggtgcgccag
    15241 cacgtagagc agcagccgcc cctccagcca ctcgcccacg aagaactccc tcgcggtgag
    15301 ggccacgaag ccgatcagcg ccagccacca gagcgtgacc gcgccgacgc ctccgtccgc
    15361 caggagcgac acggcgagct ggaccgcgac ggcggcggcg ccgagcacgc gcagctggcc
    15421 gagcgtcacg agcccgcgct ggagcacgcg atcggggtgg gccacgcggt cccgctcgta
    15481 gtccttgtgc tcgtcgagca ccctgatcat caggaagaac ccccacacgg cgaagaacgc
    15541 gccgaggtcg ccagacgaca cctcgatcgc cccctcgtgc gccagcgccc gtccgtagac
    15601 gagcgcggcg aagtacaggg agagcagcat cgcggcggtg ccggccagcg ggaagcgctc
    15661 gttggcccac gccgccatgc gccggcgcag cggagccgcc ggcgtcagct ccatggcggc
    15721 ggatgccgcc gcggcgcccg cgaccgtcgc gcgcgcggct ccgctcatgc cgccgcgcgc
    15781 catggcttcg ccgtgagcga cgcgatgcgc gagtgctcgt cggcccagta gcggaccgac
    15841 gcggggatgc acgccgccgc gtcgggcacg atggtcgtgg ccgcgagcgg ctcgtcgagc
    15901 tgtaggtacg gctcgtagtt gctgagcagg tccacgacct tgagctggcg gtccgtcagg
    15961 tgcttgcgcg cgaacggcag gaagaagcgg ttccagctcg ccggcgcgac gagccgcggc
    16021 ttcgcgaccg ggtcgagccc gcgcccggtc cgccactcgt tcagcgtgtc gacgatcgac
    16081 gtgacggccg tgtcgacggt cggcgcgctc gccccgcccg ccacggtgac cacccgcggc
    16141 tcggccggcg gcggctcctc ggcgagcgcc gcgatcgcgt gggccaggtc gtcgaccggg
    16201 atgacgtcga agagggcgtc cgggcggccg acgacggccg ggaccaggct cgagctgatc
    16261 ccccggatca ccgtgtacat ccccatgaag cgcgccgccc ggccgtcgct ccgccgcccc
    16321 atgatcaatg gggggcgcac gatggtgagc tgtgggaaca gctcgcgcgc gcggcgctcc
    16381 gcgagcgcct tcgaccactc gtacgtattg cgatagtcct ccagctccac tcccggcgag
    16441 ctgccgtcgg ggcgcgccgc gtacgcggtc gacacgtgca cgacgtgcgc cgcgcggacc
    16501 ccgagcgcgg cgagcgcctc caccgtcgcc acgttcgccc gcagcgcgtc gtcaggcgcg
    16561 agcgtccagc gcgtgtccgc cgcggagttc acgaccacgt cccactcgcc ctgcagctcg
    16621 ctgggaggcg gctcggcacc gatgtcccat gcgagaccgc cgtcgccgcc gtgccggctc
    16681 accccgacga cgcgcgtgca gccgcgcccc cgcaggacgt cgagcaactc agagccgacg
    16741 aggccggtcg agcccgtgac gaggatccgg gcgtcggtca tcggtccgtg gcggcgcgtt
    16801 ccggcgcggc gccgaggccc ggctccgcgc cctgcgaagc gatgacgttc agctcgcccc
    16861 gaaggagcag ggagcggcac aggcggcgct ggaccttccc gctcgtcgtc ttcggcacgc
    16921 tcccccgctg gcagaggacg acgagctcga ggtcgagccc gagctgggtc ccgacctcgc
    16981 gccgcagtgc gtcgattgcc gcgctcgccg cggaggcgtc ggtcagctcg gccacgaacg
    17041 ccacccgctc gtcgccgagc gtgccgtcgc cgagcgcgaa ggccgcgctc gaatgcgggc
    17101 gcaggcgctc gtcggccgcc tcggccacga cctcgacgtc gtgcggatgc aggttgcggc
    17161 cgttgaccac gagcaggtcc ttgatgcgac cgacgacgaa cagctcgccg tcgctcgtga
    17221 acccgaggtc gccggtgcgc aggaagcgct ctccgttcgc ctcgcccgcg acctgcgcac
    17281 cgaacgcccg ggcgttctcg tcgccgtgac ccaggtaccc gcgtgcgacg ctcgccccgg
    17341 cgacccagat ctcgccgatc cggcccggct cgcagcgcat cagcgtctcg ggatcgacga
    17401 tcgcgagctc gtggtccggg tccggcccgc cgcacccgac gagctccaat ccggcgcccg
    17461 ccaccggctc ggctcggccg cgttcgaggg cgcgcgcgtc gaagcgccgc agcgccgggg
    17521 gctcgtcgcg ctcgccggcg gtgaccatca gggtcgcctc ggcgagcccg tagcacggaa
    17581 ggaacgcctc gcggtggaac ccgcacggcg cgaaggcggt cgcgaatgcc tcgagcgtgc
    17641 cggcgcggat cggctcggcg ccgttgaacg cgacctccca cgagctgagg tcgagccgct
    17701 cgcgcacctc cgggccgatg cgccggacac acagatcgaa ggcgaagttc ggcccgccgc
    17761 tgatcgtggc cctgcgggtg gagattccct cgagccagcg ctccgggcgc ttgaccaccg
    17821 tcagcggcga cgtgaggacg cactcgaagc cgcggtagac cggctggagg atgccgccga
    17881 tcagccccat gtcgtggtac ggcgggagcc agatgaaccc gcggtccgcg cccgagctcc
    17941 cgagcctctg cgcgatgagc tccgagttga cgagcaggtt ggcgtgcgtg agcatcacgc
    18001 cgcgcgggtc cgccgtcgag cccgaggtgt actggaggat ggccaggtcc gcgggctcgc
    18061 gcgcgcgggc ggacgcggtg gcatcgccgt cgctcgtgca ggcgtccacc gcgagccacg
    18121 gcgcccgtgc gagcgcgtga ccctccggga cgagtcccgg cggcggggcg gtcagctcgc
    18181 ccgtcgtgag gaccgccccc accgcggcat ccgctccgat cgccagcagg cgcggcaggg
    18241 tgcgttcgag ctgccgcggg tgggggggtg gggctgacac cgcgacgacg ccggcctgga
    18301 agcacgcgaa cagcgccgcg acgtagtcga gtccgggcgg gagcaggagg agagcccgct
    18361 cgtccaccgc cagtcgctcc tcgagcatgc ccgcgatcgc cgcggagcgc gctcggagcg
    18421 cgccgtacgt catggcctcg cgctcgacgc cgtcctcgtc gaggaacgtg aacgcaagcg
    18481 cgtcgggggc atcctcggcg cgggcggcga gcgcctcggc gaacgtcctg aagtcggtgg
    18541 cccggctcac gagccgccgc cctcgaagaa gcccgcgacg agccgggtca cgtccgcgat
    18601 cgtctcgacc gcgagcgcgt cgtcggccgc gaccggaccg atccggaact cgtcctcgag
    18661 ggccgccgcc agctcgatca gggcgagcga ctcgtagccg aggtccgtga ccaggagcgc
    18721 gtcctccggc gcagccgtcg ccctgcgcgg cgacagctcg agcaccagcg ccgcgacgcg
    18781 ggcggccagg gtcgcgtcgg ccgcggccgg cgagccgctc acagcgtccc cacccgcgcc
    18841 tcgtcgaagc ggaagcgccg cgcggcgagc agcaggagca ccatcgcgct gccggtcagg
    18901 atggcgacgg ggacgatcgc gtccgcgagc ccgtcgccgt cgaggaggat gccctcgaag
    18961 cccttcatcg cccagtggct cggcgtcgcc ggggcgatcg cctccgccca ctcgggcagc
    19021 gcggcgctcg gggtcagcgc tccgccgagt gcggcgaaca ggatcgcgct gaggttggcg
    19081 accgcgagca cctgttggag cgtggaggcg tacgcgatcg tgaggaggat caacgcctcc
    19141 acccacagca cgaagctgag cgcgacggcc accagcgcga cgacggagcc gcgaacgtcg
    19201 acgcccagca gcagcacgcc gaccaggaag aggaccagct gctgcgcgca caggagcagg
    19261 aaccacagcg ccgtcttgcc gaggaccacg tcgcgcggcc tgacgggcag cgcgcggatg
    19321 cggtcccagg tgccccagac gtgctcgcgg aagaacgcga tgcccgcgta gctcgtgacg
    19381 aagaacgcga acaggacgcc catgccgggc accgcgtgct ccgcgccgtt ggcgttcgcg
    19441 tagccgtgct gctcgaggac cgcggcctgg ttcggctcga tgaacagcac gagcacgagc
    19501 ggcatcgcga agaggatgat cggcgcggtg gggtccgccc gcatgaggct gaggtcgttg
    19561 cggatcatcg tcagggctgc catgcgtccg tcccgtccgg agcgatctcg aagcgccgcc
    19621 cggtgatcga caggtatgcg gtctcgaggc tcggccgcac gatctcgacg cccctgacgg
    19681 aggcggccgc gggccccatc gacgcgaagg cccgggcgac ggccgctgtc ggctcgggcg
    19741 tgtcgatccg caccaccgac ccgccgtcgt tcagcgggcc cagcacggcg gcctcgcgcg
    19801 gcgccgggcc gtcgaagcgg atctcgacga ccgacttgcc gtgggcctcg atcagctctg
    19861 gcacccggcc ggccgcgacg attcgcccgt actcgaggat cgccgccgac gccgacagca
    19921 gctcgacctc gtgcaggtag tgcgtcgtgt agcagaccgc cgccccctcg ctcgccatcc
    19981 gcttgaccac gtccaggagc tccacccgca tgtgcacgtc gacgcccgcg gtcggctcgt
    20041 ccaggaggag gaggggcggg cggtgcagga gagcgagcgc cgtgtggagg cgacgcttct
    20101 cgccgcccga caggtcggtg gcggttcggc cgagcaggtc ggcgagcccg aagaggccgg
    20161 cgacctgctc gatgcggtcc tcgagctcgc gccggccgag ccccgccatc cggccgaaga
    20221 agcgcatgtt cgcgagcacc gtgagctgga ggtagaagcc gagcgcctgc ggtgccacgc
    20281 cgatcaggcg ccgcgcctcg agcggccggc gctgcacgtc gatgccgccc acgagcacgc
    20341 ggccggcgtc gggctcgagc aggccggcca cgatcgagac gagcgtggtc ttgccggcgc
    20401 cgttggggcc cagcagcgcg aggatctctc cccgcgcgat ctcgaggtcg actccgtcga
    20461 gggccctgac ctccccgtag gacctcgcca cgccggacag cgacagcatc ggcccgccgc
    20521 cgttcgacgc ggagcttccc aagtgcgcgc tcatttaaat aggttgacat actgatcgtc
    20581 tgatacgcaa cctgggaggc acgtccaaat cgattcgagc cggccgatcg acatatcggc
    20641 gctggagctg ctgacgacga cgcggtcggt caggcgccgc ctcgacctca cgcgtcccgt
    20701 tccgcggtcg gtggtgctcg agtgcatcag cgtcgcgacc caggcgccca gcggcggcga
    20761 tcgccagcac tggcgcttcg tcgtcgtcga cgagcccgac ctccgcgccg agatagccgc
    20821 tcactaccgc cgcgcccacc gggagcggag ggaggaggcc gcggctggat cgacgccggg
    20881 gggtggcggc aacccgcggc tcgagcgctc cgtcgacgac ctggccgagc gcctcgcgtc
    20941 cgttccggtg ctggtgatcc cgtgcgtgct ggcgccgccc tcgcccacga ccctggcggc
    21001 gcaggcgtcg ctgtggggct cgatcctccc ggccacgtgg agcttcatgc tcgccgctcg
    21061 tctccatggc ctgggcacga cgttcaccac gctgcacctc aggtacgcgg acgaggtcgc
    21121 cgcgctcctc ggcatccccg ccggcgcgac gcaggccggc ctcgtgccgg tcgcgtacgc
    21181 gcgcggcacg cggttccggc cggcccggcg caggcccgtg gagcaggtcg tgcactggaa
    21241 cgggtgggcg ggagccgcag cctcgccgca cccgtcgccg cgcaggcggt caagcgacac
    21301 aatttgctga gtcgttcctt cccgggtgga tctcgacgaa gtgtgctaaa aatgcgcagg
    21361 gaccaaatcg atcagtaaaa ctgacagaac taagtacaaa tcgtttggag ctgtcggcag
    21421 cgccggcagt cgccgtccga cgggatgctc cctgaggcgg ccggcgaggg ggccagatca
    21481 gcacggcgcg aatcatccga gagggaacgg accttcggtc gcatgacgag gacgaggtcg
    21541 acccctcccc gcgggcgtcg atcggggccg tccatgccgc ggccgtcccc accgcgctct
    21601 cctgcatccc ggcgaagcgc gcgctgttcg cggtcgacac ctacttccgt gacgcctggg
    21661 aggacgcctg cgactgcgtc gggcccgacc tcgacgactt cctctggacg cacgggctcc
    21721 tgctcgtcaa gccggatgcg gtcgcggggc gccgcctcga cgccacgctc cgctggctcg
    21781 ccgaacacga ggtcgcgatc gtcgccgccg agcgatgccg cctcacccgg cggacgatcc
    21841 gagcgctgtg gcagtaccag tggaatctcg cgacgaggga caggcgcgac ctggcggacc
    21901 tgctcatgcc ctcggtcgag tcgctcctgc tcgtgctgcg tccccgcgcg ggtcagggcc
    21961 gcgacgacgt gccgttcacg ctgaccctga atcggatgaa gggcccggcc gatcccgagg
    22021 cgcgcgcccc cgggcagctg cgccactcgc tcggcaacga gaactacctc ctcaacttcg
    22081 tgcacacgac cgacgagccg gcggacatgg tccgcgagat cggcgtgctg ttcgagccgg
    22141 ccgtgcggcg ccgcgtctac gaggcggctc gggacgggcg cgacgcggcg caggacgcgc
    22201 gccgcgaggt ggacgagctc caccgccaag tcgcgcccca ggacctgagc ctcgccggcg
    22261 cgctcgagcg cgtggccggc cgcgcgcgag cgctgcagcc gtcgcgtgag cgcgacgagc
    22321 tcctcgccgc cgtcgagcgc gtgcggagtg gcgacgaacg cgactggcgc ggcctgcgag
    22381 cgctcgcgga ccgcgcggac gtgcccctgg agcgctggga ccagatcgcg ctcggcaccc
    22441 acctgatgaa cgcaaaggag gaggggcggg agccggtcct cgcgggagtc acggcggccg
    22501 actggctggc ggcccaccgg gagtagtggt gacgacgggg accgtcacaa ccgatcgcgc
    22561 gtccgagctg gagctcagct ccgagcaccc gatcccgcgg gaggtcgtgc acaagctcgc
    22621 cgtggcggag gtcttcgtta ccgacagcgc ccggctgtcg gatgacagca tgctgctcgc
    22681 gggcgagcta ccccgcagcc actactacta cagcgacgga gggagcttcc cggcccggtt
    22741 cgacgtcatg ccgatcctcg agctgtgccg gcaggcctgc ttcgtcatgt cgcaccgcca
    22801 gctcggcgtg ccgatcgggc agcagttcat cctccggtcg ctgggcgtgg agctggaccc
    22861 ggcggcgatc ttccagcgcc aggaggagcc gctgcgcgtg accgtccggt gcgacgtgct
    22921 gaggcgctgg acggacggcg accggctcgt cggggcgctg tggcgcttca cgctgcggac
    22981 tccgcgcgag cttccggtgg cgaccgcgac ggccgccatg tcgtggatgc cgaaggagag
    23041 gtgggccgag gttcgagccg gtaaccggac gcagcgcggg ctgccgcccg agatcgccta
    23101 cgcccgcccc ggctggtcga acgtcccgcc cgcgagcgtg gggcgggagt gccgcgagaa
    23161 cgtcgtggtc tccgacatcc agctgcggga cggcggcgtg cgggcgacgc tgatcgccga
    23221 catcctccat cccgggctgt tcgaccacta cgtcgatcac ctctccggca tgctcgaggc
    23281 cgaggcgtgc cggcagctcg cgctgtgctg tgcgggaggg gggctgcccg tcagccgcga
    23341 cgtggcagtc agcgcgatcg acgtcgcctt cgccgccgtg ggtgagctcg acctgccgac
    23401 gctctgcacc gcggccgtcc agcccgtgga cggcgcgtgc gatacgcggc gcgtcgatgc
    23461 cgagctgacc cagcagggcg atccgctgtg cagggcgacg ctgacggtct cgaccggtcc
    23521 ggacgcgtga cggggcactt ccagatcgcc gaccgcgtgc tcgcgtcgcg gctgatgctc
    23581 gggaccggcg gctttcgcag cctcgagacg ctggcccggt gcctgcgcgc cgggccggtg
    23641 gagatctgca ccgtggcgct gcgccgcgtc gggtcgacgc cgcgccagcc ggtgctccag
    23701 gtgctcgacg ccgcgggcgt cagcgtcctc ccgaacacgg ccggctgcca caccgcgcgc
    23761 gacgcggtcg tcaccgcccg gctcgcgcgc gaggcgttcg agacggactg gatcaagctc
    23821 gagatcgtcg gcgaccggcg gaccctcatg ccggacgcgc gcgagctgct cgacgccgcg
    23881 gcgacgctcg ccggcgaggg gttcgtcgtc ctgccgtact cgaccgacga tccggtcctg
    23941 gcgcgccggc tggaggacgc cggatgcgcg gcgatctgtc ccggcggctc gccgatcggc
    24001 agcgggatgg gcctcaacaa cccgagcaac ctggcgctgc tgtgcgagaa cgcgggcgtt
    24061 cccgtcgtcc tggacgccgg catcggcacg gcttccgacg ctgcgctcgc gatcgagctc
    24121 gggtgcgacg ccgtgatgac cgcgaccggg atatcccgcg cgcgggaccc ggtcgggatg
    24181 gcgagggcgc tgcacggcgc cgtccaggcg ggctacctgg cgcgggtggg ggagcgcgtg
    24241 ccgcggctgc cggcaggggc ggcggcatga cggcgcgcgg cgggtcggcg ggcacgacgg
    24301 cctcggagcc cgcggtgctc gagcgcaggc tcggcgacta ccacgacgtg acgatggccg
    24361 aggtgctcgg catcgtcggg tacctgcgcg acgacggcga ctcggtgatc gtcggagggt
    24421 cgctcgccat cggcctcggc aacgggctca gcgacctgga cgtcgtggtc gccggccggg
    24481 agacgccggc atcccaggtt ccgctcgagc actgggtgaa gtcgctgcgc gtcgacgtct
    24541 ggctccggag ccagcgcgac gtcgacgccc tcttcgagcg cgccgagagc gcgctggaga
    24601 gcgagcgccc actccaggga gcgttcggca gcgtggacga ggagcaggag ctcaagctgc
    24661 tccaccgcgt cgcgttcggc ctgcattggg acgggccgga gctggtcccg tccacgcgcg
    24721 actaccccga catcgcgcgc gacctcctcg tgcgcgagta cgcggagcgg atgcgcgagt
    24781 ccgcgtgcgt cgccacgctc gcccacgccg ccggccgcga ccgggcggcg gcggtcaacg
    24841 cgcggctggc ggtcgaggag gcgatgcagg ccgtactcgc ggcgcgcggc cacccgtgga
    24901 gtggcgacaa gtggctccag gtccgcttgc aggagcacga gggcgagtgc c
//
LOCUS       DASVPL010000134         9566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_133173, whole genome shotgun sequence.
ACCESSION   DASVPL010000134 DASVPL010000000
VERSION     DASVPL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133173"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VF517_06980"
     CDS             complement(<1..1338)
                     /locus_tag="VF517_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8052719.1"
                     /translation="MAEPLTPSDLAAIGAERGPVHMHVGGVLIFDGRVEREDLIERLE
                     QRLHLIPRYRMRLESPPLGIANPVWVEDEGFDLARHVRRAALPAPGGERELTELVGHV
                     LSERLDRSRPLWQIVVVEGLERGRSAIVAKMHHALVDGVAAVDVSTVVLDPTPEGLDL
                     AAPDGQAASAPGRTARVEQLARIAAAQLAVPRRLARETVSRAFTTDPRSAARQAREAA
                     GVVAELARVRPAAPDTRLNRPIGPERLFAMARARLGDVKAVRRAAGATVNDVLLGAVS
                     LALEDFLGEEAPARAVALVPVSVRDENDRGELGNRISTVFCDLPLRGEPLDRVHEISA
                     AMDRAKDSAQVRAGALIAGATGLAPPIVSSLAVRAMSGPRIFNLVVSNVPGPQQTFYL
                     AGVPLCEVFPAVPLNPRNQALSIGIVSYAGGVQFGLLADRDAVPDVADAAAALE"
     gene            1404..1826
                     /locus_tag="VF517_06985"
     CDS             1404..1826
                     /locus_tag="VF517_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052720.1"
                     /translation="MWTRLAALVLAALALASCGGNGGGGNGGGGTPTATSFIDCFDLA
                     GFEAMKPKPREESVLAFQAKSKGYAVEPVNVQKEGMLTPHAFLVFFASAAKAREAMKE
                     LNATAYGDVPPQQRGPAVIGYGDEEDRTAVEPAIAGCL"
     gene            complement(1769..3274)
                     /locus_tag="VF517_06990"
     CDS             complement(1769..3274)
                     /locus_tag="VF517_06990"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8052721.1"
                     /translation="MTPEHVNVLVVGAGISGICAGYHLQDKCPDKSYAILESRGASGG
                     TWDLFRYPGIRSDSDMYTLGFRFRPWPDAKSIADGPSILSYLRETAAMYGIDRRIRYH
                     HRVVRAEWSSDEARWTVEAERTDTGETVVLTCDFVFVASGYYRYDEPYTPTFAGAEHF
                     GGDIVHPQRWTDDIDYTGKRVVVIGSGATAVTLVPAMAERAAHVTMLQRSPSYVVARP
                     GADPIARRLRRYLPARVVYPLVRWKNVMLTMASFQLSRRRPEVAKQLLRKGVERELPP
                     GYAIDPHFRPQYNPWDQRLCLVPDGDLFAAIRDGSASVVTDTIDSITETGIRLGSGDE
                     LEADLIVPATGLNMLPLGGIELAVDGDPVNLPDHMTYKAMMLSDVPNMAFAVGYTNAS
                     WTLKCELTTEYVCRLLNHMDKHGHSVVTPRRDPSVAAEPIIDFNSGYIQRGIGQFPVQ
                     GSRRPWRLRQNYALDLIEMRFADVDDGALEFSSYRQPAMAGSTAVLSSSSP"
     gene            complement(3348..3941)
                     /locus_tag="VF517_06995"
     CDS             complement(3348..3941)
                     /locus_tag="VF517_06995"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX8052722.1"
                     /translation="MARTVARAQGATNHSDVDHDKRTPAQWYCLVFGGTLLLVGLIGF
                     LANASFVTGKKLEDDPLLGLFDVNGWHNVVHIVSGIVLLAFAKKRATAKTAALAFGIV
                     YGLVTILGFIDGSDVLAIIPTNGADNVLHLAISALAIVSALVSSGDDRAIDRHAGADR
                     DKGRGAERTATTGHVTRTPARQGTGAGAQGTVPPTRT"
     gene            complement(4034..4897)
                     /locus_tag="VF517_07000"
     CDS             complement(4034..4897)
                     /locus_tag="VF517_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052723.1"
                     /translation="MWPASEPLPDGSTNFASLPELSYRPAGGDFGPVERIPVELPKQY
                     LTLGDDIAIGPQGDVLVAWSGTTGATFALRPAGGTFEHALSIERPGPYLLNYEPRVAF
                     DGSGTAVASWVRQMGTHDHRLVAMMRPRGAAAWGPVREIAAARHIFTPRIAFDARGAG
                     VAVWSHEQLYSSGSGRTEHGIDAAAFDPALPDIDAVKVSSRGGAPALVVDVTRATRVT
                     IAIQRLAGKRVARVGRMRAKARRGRTRIRLTRVLAERLARPGDYRFAVAVRGRDGTLG
                     APVRAAFRVKR"
     gene            5110..5271
                     /locus_tag="VF517_07005"
     CDS             5110..5271
                     /locus_tag="VF517_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052724.1"
                     /translation="MAWSWRATSRTGPTDPSANRVAATTRSSPRSDSGRVSASTQAKV
                     VFPAPSTAE"
     gene            5332..5706
                     /locus_tag="VF517_07010"
     CDS             5332..5706
                     /locus_tag="VF517_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052725.1"
                     /translation="MWNGEPPGSAESANQLTDTSPRVFEANGPITPAFPASGRGSLKA
                     APAARVEYATGSPPLPSAGPRCPHTTKASPPAPTASRGSTPRDSVSRTMSVHAAVAGA
                     ARSSAATSAASPAGVRSFIAPR"
     gene            5786..7567
                     /locus_tag="VF517_07015"
     CDS             5786..7567
                     /locus_tag="VF517_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052726.1"
                     /translation="MRRRAAVMAAVIVAAAAGAPPAHAQYEDPIAKPPPTLERWRSLA
                     RDIAAPWPAIQRRSGMLPDYMDERYGSRYGDAMMGYGLVRTGLREGNRAMVRTGLRAV
                     TFATRRPRVQSSFENFAVAATYNLARAHAADYADFRRVRAAWESWMRRSRTTRLHLPI
                     PFGNHWLIDALAVLELRRAGVRSGAGSAASLRLARRLIDVRVPSRVSRGGALLSDPPD
                     EPMAYHGLSIGFYARALQLLGRSASDRSRTTLRRMVATAAQLTAPDGDTAYWGRSLQH
                     VWSPAATAYAAEAAALLPGTSRAERAADRALAERTLARVAREYPIGARGQWIVPSLAQ
                     DFAAARPSLEGYAGAPAMSGLALTMLNWTLDGTRSGRSGSRLPADRSLARGFSHGRAR
                     FAVVRRGRVWFAVKAAHAERPYARDDLRYAFGLVSAKLRGPGGWFDLVPQRPPLGGRP
                     PVTVGPVLLGRGGRAAGFPDGERLSIGRRGVVEVRGAYRTRGGRVVRRTAWRFSTVDC
                     GVRLSFGARAGERLALTAFFRAGRPELSAKSASDGQQRVTVTPAPSSMSVARRTLASA
                     RDAHLHRLEIRVRASRAREVRVTFCGI"
     gene            7611..8423
                     /locus_tag="VF517_07020"
     CDS             7611..8423
                     /locus_tag="VF517_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX8052727.1"
                     /translation="MALERLTAANDYYQLTLALVDNRKQRQRQKRFVVQSVKAIGDAL
                     ERGWSFESLWIREGARLSSWAQDVIDRAGARRTISARVELFAALHGKEDPGELVAVLD
                     LPADRLADATLRDPALVVVCDRPSSPGNLGSIVRSADAFGASALIVTGHAADIYDPQT
                     VRASLGALFALPVAHVQSPSTAVEWLRERCPPIRLVGTSAHAVTPLEDYDFRAPVALL
                     LGSEATGLSRWWTEAADGMVTIPTAGVATSLNVAAAATAVLNEVRRQRASSP"
     gene            8459..>9566
                     /locus_tag="VF517_07025"
     CDS             8459..>9566
                     /locus_tag="VF517_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8052728.1"
                     /translation="MPADGAVDLLARTVRGVEWIAAAEIHGRLGTEATAVAHREVRFR
                     LDRLDPATLELGTVDDVFLVVGEAPAVGHTRDQLARIADAAAGCAVEPGLALLRPLRA
                     ILGVPSFDVTATFLGKRNFNRFEVEDAAGAAIARCSGWLYRSRTAGKPAPTAVTVRVH
                     LDAERTVLALRVGAAPLHRRPYRESSRSASLRPPVARCLGLLAGLGNGVALLDPFCGA
                     GTIPIEAALAEPGVVASGSDAEPEVVELARANVAAAGVQAAFDVADAAALDRPDGSVD
                     RLATNPPWGDAVEPVGSLASAWREARRVLRDGGRAAIVAPGEIVDGAARALGSPPVLR
                     QAVRILGKPAEIAVLAEDEGFAGSGLYGAELDRAR"
BASE COUNT         1196 a         3558 c         3581 g         1231 t
ORIGIN      
        1 ctccagcgcc gccgccgcgt cggccacgtc cggcaccgcg tcgcggtcgg cgagcaggcc
       61 gaactgcacg ccgccggcgt agctcacgat cccgatcgag agcgcctggt tgcgcgggtt
      121 gagcggcacc gccgggaaca cctcgcagag cggcactccg gccaggtaga aggtctgctg
      181 cgggcccggc acgttcgaga cgaccaggtt gaagatccgc ggcccgctca tcgcgcgcac
      241 ggcgagcgac gagacgatcg gcggcgccag cccggtcgcg cccgcgatca gggcgccggc
      301 gcggacctgc gcggagtcct tcgcgcggtc catcgccgcc gagatctcgt ggacccggtc
      361 gagcggctcg ccgcgcagcg ggaggtcgca gaacaccgtc gagatccggt tgcccagctc
      421 cccgcggtcg ttctcgtcgc gcaccgagac cggcacgagc gccaccgccc gcgcgggcgc
      481 ctcctccccc aggaagtcct cgagcgcgag cgagaccgcg ccgagcagca catcgttcac
      541 ggtcgcgccg gccgcgcgcc gcacggcctt cacgtcgccg agccgcgccc gggccatcgc
      601 gaacagccgc tccgggccga tcggccggtt gagccgcgtg tcgggtgcgg cgggacgtac
      661 gcgcgcgagc tcggccacca cgccggccgc ctcgcgcgcc tggcgcgcgg cgctgcgtgg
      721 gtcggtggtg aacgcgcgac tgacggtctc ccgcgccagc cgccgcggca ccgcgagctg
      781 cgcggcggcg atccgggcga gctgctccac gcgcgccgtg cgaccgggcg ccgacgcggc
      841 ctggccgtcc ggcgcagcca ggtcgagccc ctcgggcgtg gggtcgagca cgaccgtgct
      901 cacgtccacg gcggcgacgc cgtccaccag cgcgtggtgc atcttcgcga cgatcgccga
      961 ccgcccgcgc tcgagcccct cgaccacgac gatctgccac agcggccgcg agcggtcgag
     1021 gcgctcggag agcacgtggc cgaccagctc ggtcagctcg cgctcgccgc ccggggcggg
     1081 cagcgccgcg cggcggacgt ggcgcgccag atcgaagccc tcgtcctcga cccagaccgg
     1141 gttcgcgatc cccagcggcg gcgactcgag ccgcatccgg tagcgcggga tcagatggag
     1201 ccgctgctcg agccgctcga tcaggtcctc gcgctcgacg cgcccgtcga agatcagcac
     1261 gccgccgacg tgcatgtgaa cggggccgcg ctcggctccg atcgcggcca ggtcggacgg
     1321 cgtgagcggc tccgccatca ggcgcccccg ccgctgagaa ccgcgcccat ggccggagtc
     1381 tcgcagtcgc tagcttggcg agcatgtgga cccggctcgc agcgctcgtg ctggccgcgc
     1441 tcgcgctggc ctcgtgcggc ggcaacggcg gcggcggcaa cggcggcggc ggcaccccga
     1501 ccgcgacatc gttcatcgac tgcttcgacc tcgcgggctt cgaggccatg aagccgaagc
     1561 cgcgcgagga gtccgtgctc gccttccagg cgaagtcgaa gggctatgcc gtggagccgg
     1621 tcaacgtgca gaaggagggc atgctcaccc cgcacgcgtt cctcgtcttc ttcgcgagcg
     1681 ccgcgaaggc gagggaggcg atgaaggagc tgaacgccac cgcctacggc gacgttccgc
     1741 cgcagcagcg cggccccgcc gtcatcggct acggcgacga ggaggacagg accgccgtcg
     1801 agcccgccat cgccggctgc ctgtagctcg agaactcgag cgcgccgtcg tcgacgtcgg
     1861 cgaagcgcat ctcgatcagg tcgagcgcgt agttctggcg aaggcgccac ggcctgcgcg
     1921 atccctgcac cgggaactgg ccgatcccgc gctggatgta gcccgagttg aagtcgatga
     1981 tcggctcggc ggccacgctc gggtcgcggc gcggcgtcac cacgctgtgg ccgtgcttgt
     2041 ccatgtggtt gagcaaccgg catacgtact cggtagtcag ctcgcacttg agcgtccagg
     2101 acgcgttcgt gtagccaacc gcgaacgcca tgttgggcac gtcgctcagc atcatcgcct
     2161 tgtaggtcat gtggtcgggc agattgaccg ggtcgccgtc caccgcgagc tcgatgccgc
     2221 cgagcggcag catgttcagg ccggtggccg gcacgatcag gtcggcctcg agctcgtcgc
     2281 ccgagccgag ccggatcccc gtctcggtga tcgagtcgat ggtgtccgtg accaccgaag
     2341 cgctgccgtc gcggatcgcc gcgaacaggt cgccgtccgg cacgaggcag agccgctggt
     2401 cccaggggtt gtactgcggc cggaagtgcg ggtcgatcgc gtacccgggc ggcagctcgc
     2461 gctccactcc cttgcggagc agctgcttcg cgacctccgg gcggcggcgg ctgagctgga
     2521 agctcgccat cgtcagcatc acgttcttcc agcggacaag cgggtagaca acccgcgccg
     2581 gcaggtaccg ccgcagccgg cgcgcgatcg gatcggcgcc ggggcgcgcg accacgtagc
     2641 tcggcgagcg ctggagcatc gtcacgtgcg cggcgcgctc cgccatggcc ggcacgagcg
     2701 tgacggcagt ggcaccgctg ccgatcacga ccacgcgctt gcccgtgtag tcgatgtcgt
     2761 cggtccagcg ctgggggtgc acgatgtcgc cgccgaagtg ctcggctccc gcgaaggtcg
     2821 gcgtgtaggg ctcgtcgtag cggtagtagc cgctcgcgac gaacacgaag tcgcaggtga
     2881 ggacgactgt ctcccccgtg tccgtgcgct cggcctccac cgtccagcgc gcttcgtcgg
     2941 acgaccactc cgcgcgcacg accctgtggt ggtagcggat ccgcctgtcg atcccgtaca
     3001 tcgcggcggt ctcgcggagg tagctcagga tcgacggtcc gtcggcgatc gacttcgcgt
     3061 ccggccacgg gcggaaccgg aacccgaggg tgtacatgtc cgagtccgag cggatgccgg
     3121 gatagcggaa caggtcccag gtcccaccgc tcgcgccgcg cgactcgagg atcgcgtagc
     3181 tcttgtccgg gcacttgtcc tgcaggtggt aacccgcgca gatcccggag atgcccgctc
     3241 cgacgaccag gacgttgacg tgctcgggcg tcacgacggc ccactatgcc agtcgccggc
     3301 cgcggcccgc cgggtcgatg cccggcgggg ccgcgggacg gcgctgctca ggtgcgggtc
     3361 ggcgggaccg tgccctgcgc gcctgcgccg gtgccctgac gggcgggcgt gcgggtcacg
     3421 tggccggtcg tggcggtgcg ctccgcgcca cggcccttgt cgcgatcggc gccggcgtgg
     3481 cggtcgatcg ccctgtcgtc acccgacgag acgagcgcgg acacgatcgc gagcgcgctg
     3541 atcgcgaggt gcagcacgtt gtcggcgccg ttggtcggga tgatcgccag aacgtcgctt
     3601 ccgtcgatga agccgaggat cgtgacgagg ccgtagacga tgccgaacgc gagcgcggcg
     3661 gtcttggcgg tcgcccgctt cttggcgaac gcgaggagca cgatgccgga gacgatgtgc
     3721 acgacgttgt gccagccgtt cacgtcgaag agaccgagca gcggatcgtc ctcgagcttc
     3781 ttgccggtga cgaagctggc gttggcgagg aagccgatca gcccgacgag cagcagcgtg
     3841 ccgccgaaca ccaggcagta ccactgcgcc ggcgtccgct tgtcgtgatc gacgtctgaa
     3901 tgattggtcg cgccctgtgc gcgtgcgacg gttcgcgcca tggcgatacc tccatcggta
     3961 ggggtgggta cccggatggg ctacccgcgg cgttgtggcg ccgaaacaga actggcgcgc
     4021 ggcctgaccc ccgtcagcgc ttgacgcgga acgccgcgcg cacgggcgct cccagggtcc
     4081 cgtcgcggcc gcgcacggcc accgcgaagc ggtagtcgcc gggccgtgcg aggcgctcgg
     4141 cgagcacgcg tgttaggcgg atgcgcgtgc gcccgcgacg cgccttcgcc ctcatccgcc
     4201 ccactcgcgc gacccgcttg cccgcgagcc gctggatcgc gatcgtcacc cgcgtggccc
     4261 tcgtcacatc gaccaccagc gcgggcgctc cacccctgct cgagaccttc accgcgtcga
     4321 tgtcgggcag cgccgggtcg aaggcggcgg cgtcgatgcc gtgctcggtg cgaccgctgc
     4381 cgctcgagta gagctgctcg tggctccaca ccgccacgcc agcgccgcgt gcgtcgaacg
     4441 cgatccgggg cgtgaagatg tgccgcgcgg cggcgatctc gcgcacgggc ccccaggcgg
     4501 ccgccccgcg cgggcgcatc atcgcgacga gcctgtggtc gtgggtgccc atctgccgca
     4561 cccacgacgc caccgcggtg ccggagccgt cgaacgccac gcgcggctcg tagttcagga
     4621 ggtacggacc tggccgctcg atcgagagcg cgtgctcgaa cgtgccgccc gccgggcgca
     4681 gggcgaacgt cgcgccggtg gtcccgctcc acgcgaccag cacgtcgccc tgtggcccaa
     4741 tcgcgatgtc gtcgccgagc gtgaggtact gcttcggcag ctccaccggg atccgctcga
     4801 ccggcccgaa gtcgccgccg gcggggcggt acgagagctc gggcagcgac gcgaagttgg
     4861 tcgagccgtc cgggagcggc tcgctcgccg gccacatcac cgccgtgtcg ccgcggtcgt
     4921 tcgcgacgac gcgcgtcggg tagccgatcg tgtcctggaa cggccccgac agcgcgcgcg
     4981 gcgccgagaa ctcctccgag cgccgcctcg gccacgtacg cgcgccaggc gctgcgcggc
     5041 tcgccctcga gccggccgag ccaggcgatc acgacgcgcc cggaggcgtc ggcgcccacg
     5101 ctcgtcccga tggcgtggtc ctggcgggcg acctcgcgca cggggcccac cgacccgtcg
     5161 gcgaaccgcg tggcggccac cacccggtcg tcgccgcgct cggactccgg gcgggtatcg
     5221 gcctcgaccc aggcgaaggt cgtgttcccc gcaccgtcga ccgcggagta gagggggcgc
     5281 agcggcgggg tggccaccgg cccgaggtcg gcgccggcgg gacgcaggag cgtgtggaac
     5341 ggagagccgc cgggctcggc cgagtcggcg aaccagctca cggacacgtc gccgcgcgtg
     5401 ttcgaggcga acgggccgat cacgcccgcg ttcccggcca gtggccgcgg ctcgctgaag
     5461 gcggcgccgg ccgcgcgcgt cgagtacgcg accggctcgc cgcccttgcc gagcgcgggt
     5521 ccgcgatgcc cccacacgac gaaggcctcg ccgccggccc ccacggcgag ccgcgggtcc
     5581 acgccgcgcg actcggtgag ccggaccatg tcggtccacg ccgccgtggc cggcgcggcc
     5641 aggagcagcg ccgcgaccag cgcggcgagc ccggccggtg tcaggagctt catcgcgcca
     5701 cgataacccg gtgtcccggg gcggcgccag cgcggccgtg ccgaccggag cggcgccggc
     5761 cggcacgcct ctaggctgcg cccgagtgag gcgccgagca gcggtaatgg ccgccgtgat
     5821 cgtcgcggcg gcagccggcg cgccgccggc gcacgcgcag tacgaggacc cgatcgccaa
     5881 gccacccccc acgctggagc gctggcgctc gctcgcgcgc gacatcgccg cgccgtggcc
     5941 cgcgatccag aggcgcagcg ggatgctgcc ggactacatg gacgagcgct acgggtcgcg
     6001 ctacggcgac gcgatgatgg gctacggcct cgtgcggacc ggcctgcgcg agggcaaccg
     6061 cgccatggtc cggacgggcc tgcgggccgt cacgttcgcc acgcgccggc cgcgcgtgca
     6121 gagctcgttc gagaacttcg cggtcgccgc gacctacaac ctcgcgcgtg cgcacgccgc
     6181 tgactacgcg gacttccggc gtgttcgggc cgcgtgggag agctggatgc gccgctcgcg
     6241 cacgacgcgg ctgcacctgc cgatcccgtt cggcaaccac tggctgatcg acgcgctcgc
     6301 ggtgctcgag ctgcgccgcg ccggcgtgcg ctcgggggcc ggctccgcgg cctccctgcg
     6361 cctcgcgcgg cggctcatcg acgtgcgcgt gccgagccgc gtgagccgcg gcggcgcgct
     6421 gctctccgac ccgcccgacg agcccatggc ctaccacggc ctctcgatcg gcttctacgc
     6481 ccgcgcgctc cagctcctcg gccggtccgc gagcgacagg tcgcgcacca cgctgaggcg
     6541 catggtcgcc acggcggcgc agctcaccgc gcccgacggc gacaccgcgt actggggccg
     6601 gtcgctccag cacgtctggt cgcccgccgc gacggcgtac gcggccgagg ccgcggcgct
     6661 gctgcccggc acgtcgcgtg ccgagcgggc cgcggaccgc gcgctggcgg agcgcacgct
     6721 ggcgcgcgtg gcccgcgagt acccgatcgg cgcgcgcggg cagtggatcg tcccgtcgct
     6781 ggcgcaggac ttcgccgccg cgcggccgag cctcgagggc tacgcgggcg cgccggcgat
     6841 gagtgggctg gcgttgacga tgctgaactg gaccctcgac ggcacgcgct cggggcgaag
     6901 cggctcacga ctgccggcgg accggagcct ggcccgcggc ttctcgcacg ggcgcgcgcg
     6961 gttcgccgtg gtgcgccgcg gacgcgtgtg gttcgccgtc aaggcggcgc acgccgagcg
     7021 accgtacgcg cgcgacgacc tgcgctacgc gttcgggctc gtgagcgcga agctccgcgg
     7081 acccggcgga tggttcgacc tcgtgccgca gcgcccgccg ctaggcggcc ggccgcccgt
     7141 cacggtcggg ccggtcctgc tcggccgcgg gggccgcgcc gccggcttcc cggacggcga
     7201 gcggctgagc atcgggcgcc gcggcgtcgt ggaggtgcgc ggcgcctacc gcacgcgcgg
     7261 cggccgcgtg gtgcggcgga cggcgtggcg cttctccacc gtggactgcg gcgtgcggct
     7321 ctcgttcggg gcgcgcgcgg gcgagcgcct cgcgctcacc gcgttcttcc gcgccggccg
     7381 gccggagctg tccgcgaagt cggcgagcga cggacagcag cgggtgaccg tgactccggc
     7441 gccgtcgtcg atgagtgtcg cccggcggac gctggcctcg gcgcgcgacg cccacctgca
     7501 ccgcctggag atccgcgtgc gagcgagtcg cgcgcgggag gttcgcgtca cgttctgcgg
     7561 catatagtgg cgagatgacc cacgcccgct ccgccgtatc ggtgtagggc gtggccctcg
     7621 agcggctgac cgccgcgaac gactactacc agctcacgct cgcgctggtg gacaaccgca
     7681 agcagcgtca gcgccagaag cgcttcgtgg tccagagcgt gaaggcgatc ggcgatgcgc
     7741 tcgaacgcgg ctggagcttc gagtcgctct ggatcaggga gggcgcgcgg ctgtcctcgt
     7801 gggcgcagga cgtgatcgat cgcgccggcg cgcgccgcac gatctccgcg cgcgtcgaac
     7861 tgttcgcggc gctgcacggc aaggaggatc ccggggagct cgtggccgtc ctcgacctgc
     7921 cggcggaccg gctcgcggac gcgaccctgc gcgacccggc gctcgtggtg gtgtgcgacc
     7981 ggccgagcag cccgggcaac ctcgggtcga tcgtccgctc ggcggacgcg ttcggcgcga
     8041 gcgcactgat cgtcaccggg cacgccgccg acatctatga cccgcagacc gtccgcgcga
     8101 gcctcggcgc gctcttcgcg ctgccggtcg cgcacgtgca gtcgccgagc acggccgtcg
     8161 agtggttgcg cgagcggtgc ccgccgatcc gcctggtcgg gacgagcgcg cacgccgtga
     8221 cgccgctcga ggactacgac ttccgcgccc cggtggcgct cctcctgggc agcgaggcga
     8281 ccggcctgag ccgctggtgg accgaggctg ccgacggcat ggtcacgatc ccgaccgccg
     8341 gggtcgcgac ctcgctcaac gtggcggccg cggcgaccgc ggtcctcaac gaggtccgcc
     8401 gccagcgggc gtcgtccccc tgacgtgggc gacgccggcg cgcatcgcgg gatcgcgcgt
     8461 gcccgctgac ggcgcggtcg acctcctcgc caggacggtc cgcggagtcg agtggatcgc
     8521 ggccgccgag atccacgggc ggctcgggac cgaggccacc gcggtggccc accgcgaggt
     8581 ccgcttccgg ctggaccgcc tcgaccccgc cacgctcgag ctggggaccg tggacgacgt
     8641 cttcctcgtc gtcggcgaag cgccggcggt cgggcacacg cgcgatcagc tcgcgcgcat
     8701 cgccgacgcc gccgccggct gcgccgtcga gccggggctt gcgctgctga ggccgctgcg
     8761 cgcgatcctc ggcgtgccga gcttcgacgt tacggccacc ttcctcggaa agcgcaactt
     8821 caaccgcttc gaggtcgagg acgccgcggg cgccgcgatc gcgcggtgct cgggctggct
     8881 ctacaggagc cggaccgccg gcaagccggc gccgaccgcg gtgaccgtgc gcgtgcacct
     8941 cgacgccgag cgcaccgtgc tcgcgctgcg ggtcggcgct gcgccactgc accggcgccc
     9001 gtaccgcgag tcctcgcgca gcgcctcgct gcggcctccg gtggcgcgct gcctggggct
     9061 gctcgcgggc ctcggtaacg gcgtggcgct gctcgacccg ttctgcggcg cgggcaccat
     9121 cccgatcgag gccgcgctgg ccgagcccgg agtggtcgcc tccggctcgg acgccgagcc
     9181 ggaggtcgtg gagctggcgc gggcgaacgt ggcggcggcc ggcgtgcagg ccgccttcga
     9241 cgtggcagac gcggcggcgc tcgaccgacc cgacggctcg gtcgaccggc tcgcgaccaa
     9301 ccccccgtgg ggggacgcgg tcgaaccggt cggctcgctc gcatcggcct ggcgcgaggc
     9361 ccggcgggtg ctgcgcgacg gcggccgggc cgcgatcgtg gctccggggg agatcgtgga
     9421 cggcgcggcg cgcgcgctcg gatccccgcc ggtgctgcgc caggcggtgc ggatcctcgg
     9481 aaagcctgcc gagatcgccg tgctcgccga ggacgagggg ttcgcgggca gcgggctcta
     9541 cggggccgag ctcgaccgcg cgcggc
//
LOCUS       DASVPL010000135         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_133206, whole genome shotgun sequence.
ACCESSION   DASVPL010000135 DASVPL010000000
VERSION     DASVPL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133206"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VF517_07030"
     CDS             <1..482
                     /locus_tag="VF517_07030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8052729.1"
                     /translation="SVLACDGQRPVWEERTPHDVVAAAGAADHCRIDLEVDARWWLLD
                     LLTERPDQWIDLAYVDAAHTVEVDSFLVSALWRHIRPGGVLVLDDLDWTASLHARPGA
                     VFSRPDRSHVRALYDYLRALPDVADATEWGREEVGWTWALLLKASGSGPADLASLLR"
     gene            complement(479..1378)
                     /locus_tag="VF517_07035"
     CDS             complement(479..1378)
                     /locus_tag="VF517_07035"
                     /inference="COORDINATES: protein motif:HMM:NF039471.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6492 family protein"
                     /protein_id="HEX8052730.1"
                     /translation="MDVDARPSHSDGPASHGFTLVTVAHGEDTAFMLRQARSLRLYLD
                     RSLASEVVVVENPDPGSPVDWRDPLRREYGDLAGRVRFLDAGELGGIPRHVSGWVAQQ
                     ILKLMAARVVATDRYVVLDAKNHLLHPLARDYFEAADGRLRSRLVDYADHPLRPRLQD
                     TLAYFGLAAERHVGSFLPTTTPFAFSTAVVRDAIAHLEAQEGAPFPLAFVHSGRSFTE
                     FFLVGAYVLATGAALEDLYEPSGTRCETVWPESSESDVLAAVARCESEARPFFAVHRR
                     ALPQLSAEARLAIDSLWRRRGLA"
     gene            1406..2341
                     /locus_tag="VF517_07040"
     CDS             1406..2341
                     /locus_tag="VF517_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8052731.1"
                     /translation="MDSMTEAELASFADVVVEREDSPPGPDAMVARLRGCDAVLSLNG
                     YGASDLTPDVLRAAGSVRMICVAHYWGGHFDAIEDETGIPVVEGSNAGTLAVAEWNVA
                     AALMGVRRMHLFDRALKAGSPWGEPRRSAGLLAESTVGVVGLGRTGRYTARCFRALGA
                     RVIAHSASVPAEQAEAEGIALVPLDELLASADVVSLHHRVAGGTRGALGAREFGLLRD
                     GCVFVNSARAELYDEAALVRELRTGRFSAYIDVFADEPLPRDHPFRSMDNVVLTPHVA
                     GNNAPMFRRCGREAVATLREFAEGRTPDDRRYAYP"
     gene            2355..>3173
                     /locus_tag="VF517_07045"
     CDS             2355..>3173
                     /locus_tag="VF517_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8052732.1"
                     /translation="MLREPAPPPGAGRLRYAVIGAGSVARTHLREVAGRAGVAVVGMA
                     DPADPRRWRAQHDGAPRFGDPARMLAETRPHLVSICTPTKFHHELTLLALRARAHVIC
                     EKPMAMTVAEAEEMERARAATRRLGAVNFSYRNAPALRYARALVAGGEIGRVTRVRGA
                     YLQSFLAAPDSGWTWRSDVELAGYGALGDLGVHLIDAARFVTGLEFERVIGVKQTLIA
                     EKRDGAGTARRVTSDTTASFLAALGDGVTATFETSQVVAGYGDSFRLEVSGDGGT"
BASE COUNT          409 a         1151 c         1203 g          410 t
ORIGIN      
        1 gctccgtgct ggcgtgcgac ggtcagcggc ccgtctggga ggagcggacc ccgcacgacg
       61 tggttgccgc ggcgggtgcc gcggaccact gccgcatcga cctcgaggtc gatgcccgct
      121 ggtggctgct cgatctgctc acggagcggc cggaccagtg gatcgacctg gcctacgtgg
      181 atgcggcgca cacggtcgag gtcgactcgt tcctggtctc ggccctctgg cggcacatca
      241 ggccgggggg cgtgctcgtg ctcgacgacc tcgactggac cgcgtccctc cacgcgcggc
      301 cgggggccgt cttcagccgc cccgaccgca gccacgtgcg cgcgctctac gactacctgc
      361 gcgcgctgcc cgacgtcgcg gacgcgaccg agtggggccg cgaggaggtc ggctggacct
      421 gggccctgct gctcaaggcg tccggctccg gaccggccga cctggcgtcc ctcctgcgct
      481 aggcgagccc gcgccggcgc cagagcgagt cgatcgccag ccgcgcctcc gcgctcagct
      541 ggggcagcgc gcgccggtgc accgcgaaga acgggcgggc ctccgactcg cagcgggcga
      601 cggccgcgag cacgtcgctc tcggagctct cgggccagac cgtctcgcag cgcgtcccac
      661 tcggctcgta caggtcctcg agcgccgcac ccgtagccag cacgtacgcg ccgaccagga
      721 agaactcggt gaagctgcgc ccggagtgca cgaaggcgag cgggaacggc gctccctcct
      781 gagcctccag gtgggcgatc gcgtcgcgca ccacggcggt cgagaaggcg aacggggtcg
      841 tggtcggcag gaacgaccct acgtggcgct cggccgccag gccgaagtac gcgagcgtgt
      901 cctggagccg cggccgcagc gggtggtccg cgtagtcgac gagccgcgag cgcagcctgc
      961 cgtcggccgc ctcgaagtag tcgcgggcga gggggtggag gaggtggttc ttggcgtcga
     1021 gcacgacgta gcggtcggtc gccaccacgc gcgcggccat cagcttcagg atctgctgcg
     1081 cgacccaccc gctcacgtgg cgcggaatcc cgcccagctc gcccgcgtcg aggaaccgca
     1141 cccgcccggc caggtcgccg tactcgcgcc gcagcgggtc gcgccagtcc acggggctgc
     1201 ccgggtccgg gttctcgacc acgaccacct ccgaggccag ggagcggtcg aggtagaggc
     1261 gcaatgagcg cgcctggcgc agcatgaacg cggtgtcctc gccgtgcgcg accgtgacga
     1321 gggtgaatcc gtgagaagct ggcccatcgg aatggctcgg ccgcgcgtct acgtccatcg
     1381 ctcaggtcac tggtactcgc tgttcatgga cagcatgacg gaagccgagc tggcctcctt
     1441 cgcggacgtc gtggtggagc gcgaggactc cccgccgggg cccgacgcga tggtcgcgcg
     1501 gctgcgcggc tgcgacgcgg tgctgtcgct gaacggatac ggcgcgagcg acctgacgcc
     1561 cgacgtgctg cgcgccgcgg gcagcgtccg catgatctgc gtcgcgcact actggggcgg
     1621 gcacttcgac gcgatcgagg acgagacggg catcccggtc gtggagggct cgaacgctgg
     1681 cacgctcgcg gtggccgaat ggaacgtcgc cgccgccctg atgggggtcc gccgcatgca
     1741 tctgttcgac cgcgccctca aggccggctc gccgtggggc gagccgcggc gcagcgcggg
     1801 gctgctggcg gagagcacgg tgggggtcgt cggcctcggc cgtaccggcc gctacaccgc
     1861 gcgctgcttc cgcgccctgg gcgcgcgcgt gatcgcgcac agcgccagcg tgcccgcgga
     1921 gcaggccgag gccgagggca tcgcgctcgt gccgctcgac gagctcctcg caagcgcgga
     1981 cgtcgtctcg ctgcaccacc gcgtggccgg cggcacccgg ggagcgctcg gcgcgcgcga
     2041 gttcgggctg ctgagggacg gctgcgtctt cgtcaactcc gcgcgcgcgg agctctacga
     2101 cgaggcggcg ctcgtcaggg agctgcgcac cggtcgcttc agcgcgtaca tcgacgtctt
     2161 tgcggacgag ccgctgccgc gcgatcatcc gtttcgctcg atggacaacg tggtcctcac
     2221 cccgcacgtc gcgggcaaca acgcgcccat gttccgccgc tgcgggcgcg aggccgtcgc
     2281 gacgctgcgc gagttcgccg aggggaggac gcccgatgac aggcggtacg cgtacccgta
     2341 ggctcgccgc gcgggtgctc cgcgagccgg cgccgccgcc cggcgccggg cggctgcgct
     2401 acgccgtcat cggcgcgggc agcgtcgcgc gcacgcacct gagggaggtc gccggcaggg
     2461 cgggcgtcgc ggtggtcggc atggccgacc cggccgaccc gaggcgctgg cgcgcgcagc
     2521 acgacggcgc cccgcggttc ggcgacccgg cgcggatgct cgccgagacg cgcccgcacc
     2581 tggtctcgat ctgcacgccc acgaagttcc accacgagct caccctgctc gcgctgcgcg
     2641 cacgcgcgca cgtgatctgc gagaagccga tggcgatgac cgtggccgag gcggaggaga
     2701 tggagcgcgc gcgggccgcc acccgcaggc tcggcgccgt caacttctcc taccgcaacg
     2761 ccccggcgct gcgctacgcg cgcgcgctag tcgccggcgg cgagatcggc cgggtgacgc
     2821 gcgtgcgcgg cgcctacctc cagagcttcc tcgccgcccc ggacagcggc tggacgtggc
     2881 gcagcgacgt ggagctggcg ggctacggcg cgctcggcga cctcggggtg cacctgatcg
     2941 acgccgcccg cttcgtgacc ggcctcgagt tcgagcgcgt gatcggcgtg aagcagacgc
     3001 tgatcgcgga gaagcgcgac ggcgccggca cggcgcggcg ggtcacgagc gacacgaccg
     3061 cttccttcct cgccgcgctc ggcgacgggg tgacggccac gttcgagacc tcgcaggtgg
     3121 tggcgggcta cggggactcg ttccggctgg aggtctcggg cgacggcggg acg
//
LOCUS       DASVPL010000136         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_253552, whole genome shotgun sequence.
ACCESSION   DASVPL010000136 DASVPL010000000
VERSION     DASVPL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253552"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="VF517_07050"
     CDS             complement(<1..1847)
                     /locus_tag="VF517_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052733.1"
                     /translation="MTPAPGTPDLPALAFARAPSVPPPGSIQFYDGYFPALDAGTYTI
                     GIDQTVAAPSGTKPQYGATQVFEVVAPEFAIDPGVVHTAYPPEGATGVYDQQLPFVVL
                     GDPSLPWERSIVPGGGAPDSSDPTPWLALVVFAEGEVALQPGSSSPVATTTVSDLLAT
                     DSNVLKPQIPTADVSAAVLASQCQTITIPGAVFNAVMPGKDDLRYLAHTRAVSHPDEG
                     DVLLSVALSNRLPVATTAPLRFYAQLVSVEGFQDYLGPNAKPIPPKASGGGLMDVQLV
                     SLYSWSFTSQPAAGLSFKDLVTGLIASEQVTPALRLPAPPSVQLPPAAAGRLRDGYAP
                     LPFVTGAGEQSIAWYRGPFTPVVPQPLPQVGDPPVDVGEAASADALMIYLAEQGLFDL
                     SYAAAWNIGRQLALADAQFAQAMSQYRREARGALGRLSQRMAAPHLADAEEPADLLAP
                     NASRQRFAARVGDGLAARWTEAMGAARSGERPPADVAHRVRPAPRRAAVHPATVLAQP
                     WAGEVLGDQVAESADQVSAWLAKLTLLGSVPFSYLVPDARMLPAESVRFFYVDQGWID
                     ALVAGATSIAVHSTADVALLGALRPRLQQAVAVQRVGLGAPAGGPGMTG"
     gene            complement(1844..>2069)
                     /locus_tag="VF517_07055"
     CDS             complement(1844..>2069)
                     /locus_tag="VF517_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052734.1"
                     /translation="AQPVGDVPVNSPGTVGKIADPNTGIASTAVTATRKAIYDALLGV
                     NYVPAGYDDSLGDFSTTIDCALAADPLLVS"
BASE COUNT          262 a          748 c          805 g          254 t
ORIGIN      
        1 atcccggtca tgccggggcc gcccgcgggt gcgcccaggc cgacgcgctg caccgccacc
       61 gcctgctgga gccgcggccg cagcgcgccg agcagcgcga cgtccgcggt cgagtgcacg
      121 gcgatgctcg tcgcgccggc cacgagcgcg tcgatccagc cctggtccac gtagaagaac
      181 cggaccgact ccgccggcag catccgcgcg tccggcacca ggtacgagaa cggcaccgac
      241 ccgagcagcg tcagcttcgc gagccaggcg ctcacctggt cggcggactc ggccacctgg
      301 tcgccgagca cctcgccggc ccacggctgc gcgagcacgg tggcggggtg caccgcggcg
      361 cggcgcgggg cggggcggac gcggtgggcc acgtccgccg gcggccgctc gccggagcgg
      421 gcggcgccca tggcctcggt ccagcgcgcc gcgagcccgt cgccgacgcg cgcggcgaag
      481 cgctgccgcg acgcgttcgg cgcgagcagg tcggccggct cctcggcgtc cgcgaggtgc
      541 ggcgcggcca tgcgctgcga gagccggccg agcgcgccgc gcgcctcgcg ccggtactgg
      601 ctcatcgcct gcgcgaactg ggcgtccgcg agcgcgagct gcctgccgat gttccacgcc
      661 gcggcgtagc tgaggtcgaa caggccctgc tcggcgaggt agatcatcag cgcgtcggcg
      721 ctcgccgcct cgcccacgtc gaccggcggg tcgccgacct gcggcagcgg ctggggcacc
      781 accggcgtga acggcccgcg gtaccaggcg atgctctgct cgccggcgcc ggtcacgaac
      841 ggcagcgggg cgtagccgtc ccggaggcgc ccggcggccg cgggcgggag ctgcacgctg
      901 ggcggcgccg gcagccgcag cgcgggcgtg acctgctcgc tcgcgatgag cccggtcacg
      961 aggtccttga agctcagtcc ggccgccggc tgcgaggtga acgaccagct gtagagcgac
     1021 accagctgga cgtccatgag gccgccgccc gacgccttcg gcgggatcgg cttggcgttc
     1081 gggccgaggt agtcctggaa gccctcgacc gagacgagct gcgcgtagaa gcgcagcggc
     1141 gccgtcgttg cgaccggcag ccggttcgac agcgcgaccg agagcagcac gtcgccctcg
     1201 tcggggtggc tcacggctcg cgtgtgggcg aggtagcgca ggtcgtcctt gcccggcatg
     1261 accgcgttga agacggcgcc cgggatcgtg atcgtctggc attgcgacgc gagcaccgct
     1321 gccgacacgt ccgccgtcgg gatctgcggc ttcagcacgt tcgagtcggt ggccagcagg
     1381 tcggacacgg tcgtggtcgc caccgggctg ctcgaccccg gctggagcgc gacctcgccc
     1441 tccgcgaaga ccaccagcgc gagccacggg gtcgggtcgc tcgagtccgg cgcgccgccg
     1501 cccgggacga tcgaccgctc ccacggcagc gacgggtcgc cgagcaccac gaacgggagc
     1561 tgctggtcgt acacgccggt cgcgccctcg ggcgggtagg ccgtgtggac cacgccgggg
     1621 tcgatcgcga actcgggcgc gaccacctcg aagacctgag tcgccccgta ctgtggcttc
     1681 gtgccgctcg gcgcggccac ggtctggtcg atcccgatgg tgtacgtgcc cgcgtcgagc
     1741 gccgggaagt agccgtcgta gaactggatg ctgccgggcg gggggacaga cggcgcgcgc
     1801 gcgaacgcga gcgcggggag gtcgggtgtc ccgggcgccg gcgtcatgac acgagcagcg
     1861 ggtcggccgc cagggcgcag tcgatcgtgg tgctgaagtc gccgagcgag tcgtcgtagc
     1921 ccgccggcac gtagttgacg ccgaggagcg cgtcgtagat cgccttgcgg gtggcggtga
     1981 ccgccgtgga cgcgatgccg gtgttcggat cggcgatctt cccgacggtg ccggggctgt
     2041 tgaccggcac gtcgccgacc ggctgcgcc
//
LOCUS       DASVPL010000137         2668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_173363, whole genome shotgun sequence.
ACCESSION   DASVPL010000137 DASVPL010000000
VERSION     DASVPL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173363"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VF517_07060"
     CDS             complement(<1..789)
                     /locus_tag="VF517_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8052735.1"
                     /translation="MYRGLACVLLLLMLPATPAVAAFPEGPPNDPLYDASPLPNATAE
                     QWDLSSPALGFDRGISVDRAWPLTLGEGVTIAEIDAGVDYGHPDLAGRWAPGGYDFYA
                     RDPDPTSDTQVSHGTNVAGVLGATADNGLGIAGIAPRSPILPIRTSDNIIHQGARIAE
                     AIVYATDRGARAISMSLGADSFPGSLRRAVSYAHRHGVVMAVASGNEFHFHHHQPQVL
                     DGVLAVGGVNPDSANLSARDQTLAQAASDFTTRASYSNYGAHLDV"
     gene            862..2055
                     /locus_tag="VF517_07065"
     CDS             862..2055
                     /locus_tag="VF517_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8052736.1"
                     /translation="MTTRRFVAETEADGTMAAFVEALARNEERFVEEMSSAIRSEVAE
                     AAKVPEDSLEECIRIELRYALIAMVERRLPNPTEVADTAAMAANFARAGVTIEALHEG
                     RRVATRRTCELMREIGDECGLDSDTQVECIYRVWEWADATQVSDAEVYRARERELQGG
                     GEPERTRFVRALLHGTLAPTEAAARATAYGLLPSGLYLAFRARPAPGVDQQTLARSIE
                     TTGGNDGFGVVVASVDGDLCGALTRAPRINGLGIVGLGPPAELTRLTDSFQLANRALD
                     TALAFGTDGVVTMDDLSLRPAILAESHLGERLVQRYLEPLQELGEFGVTLEATVREYL
                     AHGMRIDESAKALFVHANTLRHRLDRFQQLTGADLRNTQHVLELWWALERRRLGRDPS
                     AARQS"
     gene            complement(2052..>2668)
                     /locus_tag="VF517_07070"
     CDS             complement(2052..>2668)
                     /locus_tag="VF517_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8052737.1"
                     /translation="VKPSDEHRRLRVLLVDDEDVVHWGFRMLLSTETWVERFDAAHNS
                     EAALNLAARYEHHVALIDLMLGGESGADVCEALRETSPTTRVLLMSGVERISAQSARA
                     VGASGFVPKGWGAPDIAGAARMVGLGMTVFTSEGHHPPNLLTEREREVLAMIAGGATN
                     REIAERLFLSPHTVKDHTSTLYKKMNARNRAEAIVRAQRMGLLA"
BASE COUNT          367 a          975 c          954 g          372 t
ORIGIN      
        1 cacgtcgagg tgggcgccgt agttcgagta cgaggccctg gtcgtgaagt cgctcgccgc
       61 ctgcgcgagg gtctgatcgc gcgccgacag gttcgcgctg tcggggttca ccccgccgac
      121 cgcgagcacg ccgtcgagga cctgcggctg gtggtggtgg aagtggaact cgttgccgga
      181 cgccacagcc atcaccaccc cgtgccggtg ggcgtagctc acggcccgtc gcagcgatcc
      241 cgggaacgag tcggcgccca ggctcatgct gatcgctcgc gcgccccggt cggtcgcgta
      301 cacgatcgcc tcggcgatcc gggcgccctg gtggatgatg ttgtcggacg tccggatcgg
      361 gaggatcggc gagcgcggcg ctatcccggc gatcccgagc ccgttgtcgg ccgtcgcgcc
      421 cagcacgccg gccacgttcg tcccgtggct gacctgcgtg tcggaggtcg ggtccgggtc
      481 gcgcgcgtag aagtcgtagc cgcccggcgc ccagcgcccg gcgaggtcgg gatgcccgta
      541 gtcgaccccc gcgtcgatct ccgcgatcgt caccccctcg ccaagggtca gcggccaggc
      601 gcggtcgacg gagatgccgc ggtcgaaccc gagcgccgga ctggagaggt cccactgctc
      661 cgccgtggcg ttcggaagcg ggctggcgtc gtagagcggg tcgttcggcg gcccctccgg
      721 gaacgccgcg acggcggggg tggcggggag catcaggagc agaagcacgc atgcaagccc
      781 gcggtacatg gcgcaaaggt acgcctcggc ggcctccggg cggcccgtgg ccgcgctggc
      841 gccgtttaga cttgaggcag gatgacaacg cgtaggttcg tcgctgagac ggaagccgac
      901 ggcaccatgg cggccttcgt cgaggcgctg gcccggaacg aggagcgctt cgtagaggag
      961 atgtcctcgg ctatccggag cgaggtcgcc gaggccgcca aggtgccgga ggattcactc
     1021 gaggagtgca tccgcatcga gctgcgctat gcgctcatcg cgatggtcga gcgcaggctg
     1081 ccgaacccca ccgaggtcgc ggacacggcg gcgatggccg ccaacttcgc ccgggcgggc
     1141 gtcacaatcg aggcgctgca cgagggccgc cgcgtggcca cccgccgcac ctgcgagctg
     1201 atgcgcgaga tcggcgacga atgcgggctc gactccgaca cgcaggtcga gtgcatctac
     1261 cgggtgtggg agtgggccga cgcgacccag gtgagcgacg cggaggtcta ccgcgcccgc
     1321 gagcgcgagc ttcagggcgg cggcgagccg gagcgcaccc gcttcgtgcg cgctctcctc
     1381 cacggcacgc tcgcgcccac cgaggccgcc gcccgcgcca cggcctacgg cctgctcccg
     1441 agcgggctct acctcgcctt ccgcgcgcgg cccgccccgg gggtcgacca gcagacgctc
     1501 gcgcgctcga tcgagaccac cggcggcaac gacggcttcg gcgtcgtggt cgccagcgtg
     1561 gacggcgacc tctgcggcgc tctcactcgc gccccgcgga tcaacggcct cggcatcgtg
     1621 ggcctcggtc cgcccgccga gctcacccgc ctcaccgact cgttccagct cgccaaccgc
     1681 gcgctcgaca ccgccctcgc gttcggcacc gacggcgtcg tgaccatgga cgacctgtcg
     1741 ctgcgcccgg cgatcctcgc cgagtcgcac ctgggcgagc ggctcgtgca gcgctacctc
     1801 gagccgctcc aggagctagg cgagttcggc gtcaccctgg aggccacggt ccgcgagtac
     1861 ctcgcgcacg gcatgcgcat cgacgagagc gccaaggcgc tgttcgtcca cgcgaacacc
     1921 ctgcgccacc ggctcgaccg cttccagcag ctcaccggcg cggacctgcg caacacccag
     1981 cacgtgctgg agctctggtg ggcgctggag cgccgccggt tgggtcgcga cccgagcgct
     2041 gcgcgccaga gctaggcgag caggcccatg cgctgggccc ggacgatcgc ctcggcccgg
     2101 ttccgggcgt tcatcttctt gtagagcgtg ctcgtgtggt ccttgaccgt gtgcggcgag
     2161 aggaacagcc gctccgcgat ctcgcggttg gtcgcgccgc cggcgatcat cgccagcacc
     2221 tcgcgctccc gctcggtgag caggttcggc gggtggtgcc cctccgaggt gaacacggtc
     2281 atcccgagtc ccaccatccg cgcggctccg gcgatgtccg gcgcgcccca gcccttcggg
     2341 acgaacccgg aggcaccgac ggcccgcgcc gactgcgccg agatccgctc cacgcccgac
     2401 atcagcagga cgcgcgtggt aggcgaggtc tcgcgtagcg cctcgcagac gtccgcgccc
     2461 gactcgccac cgagcatgag gtcgatcagc gcgacgtggt gctcgtagcg ggcggccagg
     2521 ttcagcgccg cctccgagtt gtgggccgcg tcgaagcgct ccacccacgt ctcggtggac
     2581 aggagcatcc tgaatcccca gtggacgacg tcctcgtcgt cgaccagcag gacgcgcagg
     2641 cgccgatgct cgtcgctcgg cttgaccg
//
LOCUS       DASVPL010000138        58091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_212092, whole genome shotgun sequence.
ACCESSION   DASVPL010000138 DASVPL010000000
VERSION     DASVPL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 58091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58091
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212092"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF517_07075"
     CDS             complement(<1..207)
                     /locus_tag="VF517_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052738.1"
                     /translation="MREATSRAETLRRLDSLLGDMPYTHTPACHLLFDLASARDAPQL
                     VEIACGYGKATAYLGAAARRRGGSV"
     gene            255..1769
                     /locus_tag="VF517_07080"
     CDS             255..1769
                     /locus_tag="VF517_07080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX8052739.1"
                     /translation="MAVTEYRRRGRWIDHWDPEDERFWESTGRRIARKNLALSIFAEH
                     LGFSIWVLWTIIVINLGNAGITLSLAEQFWLTAVPNLIGSTLRLPYTFAVPRFGGRAW
                     TTFSAGLLLIPALLVAIVVPSGWLADQSHSTQFWVLVACAATAGVGGGNFSSSMANIS
                     FFYPEGRKGFALGLNAAGGNLGVALTQLLVPLAIIVGVPAAAVKLPAHDVHLAYAGLV
                     WLPLIVIAALGAWLYMDSLSQARSDQRSYLLAFRQGQTWIMSLLYIGTFGSFIGYSFA
                     LPLVIKNTFPEFLADHPFIATYLAGFAFLGALVGSVARPVGGWLSDRVGGARVTLGVF
                     LGMSVLTMLAIVGVREHSFPLFFSSYMVIFLLAGMGNGSTYRMIPAIFDALGRREAEE
                     NGYSGERLREMLLRYKRQAAAAIGIAGAIGAFGGFMIQMVFRQASLEVSALVKSADGV
                     AAKAAVAQAHAEWSVPALGAFLGAYVLFAGMTWFFYLRRSFAVEKVPSLASASV"
     gene            complement(1783..2544)
                     /locus_tag="VF517_07085"
     CDS             complement(1783..2544)
                     /locus_tag="VF517_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8052740.1"
                     /translation="MSPRPEDAWLHRPEPQPDPPMRLICLPFAGGMAQSFHPWAGPLS
                     TVAELCAVALPGRGTRVQEPSIDRIDRLTTFVSAAVRGLLDRPYALFGHSMGALLAYE
                     LARELDRAGAPAPLRLFVSGFRAPHLPARGDPLHVLPDDELTARLDEFGGTPPKVMES
                     NAFMGLFLPPTRADLAACETYEHVPGPALATPITAFGGRADRLVSEDELRAWEQHTSG
                     AFMLRLFEGGHFYLRDREAEFVEVLGCSLEDELVR"
     gene            complement(2545..11025)
                     /locus_tag="VF517_07090"
     CDS             complement(2545..11025)
                     /locus_tag="VF517_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8052741.1"
                     /translation="MIELLRQRAADEPDRRLFTFLRDGELDEEHVTYGELDEAARMLA
                     VGLAERTQPGDRALLVYAPGLDYIRAFFGCLYAGVCAVPLYPPGLNRLAPPLARFADD
                     CDAAVILTVRDFEEMVRAQPGVESRPTFVTDDVSPDAADSWQRPDVDDSALAFLQYTS
                     GSTSFPKGVMLSHRNLVKNIGMIHDGTFQGEVSRSQGVSWLPPYHDMGLIGGVLWPIG
                     IGFHAVHLSPLHFLERPMRWLEALSRFKATVTGAPNFAYELATRRARDEDVAGLDLSS
                     MHIFMNAAETVRPDTIDAFCERFGPCGARREQFLPAYGLAEATLMVSSGRPRPIHADS
                     RALERGEVVIHDGPGSGVRSLESLGTPWCGTRIEVVDPETCERVPEGRVGELWVSGPS
                     VARGYWGKPEETERTFQARIAGEEGDTRWLRTGDRGFFESGEFVWTGRLKDLMVVRGR
                     NVHPEDVELTVEASHSALRPGCGVVFAIDGDDEQALTIVQELRGGADEQAAEAAEAIR
                     RAVSREHQVEVDRVVLIEAGSGPKTSSGKKQRRATCELLQAGELPIIHDAEAKLAAAA
                     SVEIGDSGADPAPAAAEQRAPRSSAEIAHWLTDHLAGALGVDASAVDTRMPLIDVGLS
                     SVQAAELAEELGRWLGRDLSMTLIFDHPTIDRLAEHLGEDAAAPADPPATGRRVSERA
                     RSNGDGTQEPVAIVGMGCRFPGAPDPDALLEMLRTGGDGISEVPLDRWDADALFDPEL
                     KSPGKVRTRWGGFLDGIDQFDPQFFGITPREARSIDPHQRLLLEVAWEALEDGALDPH
                     RLRGKDAGVFVGIGGIDYQARLARRLKMIDPHSGVGNSHSIAANRLSFLLDFHGPSVA
                     VDTACSSASVAIHLACQALENDECELALAAGANVILTPETTIAFSAARMLSPDGRCRT
                     FDEAANGYVRSEGCGVIVLKRLSDAIRDGDRIRGVIRGTAVNQDGQTNGLTAPSAKAQ
                     QRVIRSALARAGIEAHDLGYVEAHGTGTPLGDPIEVEGIAGAIGPRRDASHRLPMGSV
                     KGNIGHTETVAGVAGVIKTVLALENREIYPQPHFEQLNSRIKLDDTPLEVARELQEWE
                     ANGGPRLAGVNSFGFGGTNAHVVVEEAPAPLPARNRGERPRQVLTLSARSSDALGELA
                     GRYSRACSEMPEESFLDACYTAQLGRAHFEHRAAIVAANGAEACKRLDALASGEAGAT
                     AAGHVHNGVRPMVGFLFTGQGAQYLEMGRALRESEPTFRRALTDCAEMMDAYLDHDVL
                     DVIDGKAPDCLLDDTRYTQPALFVVEWALAELWRSWGIQPDAVLGHSVGEYVAACVSG
                     AMDLEDGVMLIAERARLMSDLPAGGGMASVLASEEQAEKTIEEVGARLTLAAVNGPTN
                     VTIAGAAAELDKVTERFEAEGVSTRRLAVSHAFHSELVEPIIDDLERMAGRFDYREPR
                     IPLISNVTGRPLDRAPDARYWRDHTRSPVRFADSVRAMLGLGVEVFVEVGPHPSLISL
                     GKHCVGGAGRAWVPSLRRNQDDLSTALGSLATLYVNGVEPDWKAFHKRWHRRPVQLPT
                     YAFQRTRHWIDIDPEGEGAEDAGAGASASSVADHPLLGRRLATGNVVLEGRIGPERHP
                     YLDDHRVQGRAMLPATAYLEMALAAAGHGSSEAPKVLRDVVLRQPLIFGQDAARVQVS
                     VVPLEGGESEVTVQSARGDGSSNGSTSWTLHARATAGGAGAEAGSADIDELRERCGEE
                     IPAERIYDGLRARGLEYGPAFRGLRRAWRGERDVIGEVELPEEATGDGYRAHPALLDA
                     CLHAIAAGQPEPLAGEGPMIPIAIDSVTFHAPLPSSVVVHAHMAGAPDPGEVITDVAV
                     LDAGGATVAELRGLHLLAIDDRGAAGGSDDPARWLHELAFEASDASSSAVPADGGWLV
                     LADRGGIGQALAERIRPSGGRCVLAFRGEALERLESDDFVVAPDGVTTVIDQAFGEGE
                     SPSGVIHLWGLDAPEPGEDDWLEGSLGAGCDSLMNVVQALVAGKRRTRLEVVTRGAHV
                     VGSGDAPSPTQAMLFGFTRSLALEHPELKVAAVDIDPAADQRGAARSLAAELASDPEP
                     VVALRGGKRHVGRLVRRDASHDSTDAASSNGGPPALAAPRGAFKLRTRTAGVLDALEL
                     APAERHPPGPGLVELEVRATGLNFRDVMKVMGVYPGTAADRRSLGDECSGVVVAVGDG
                     VEHVAVGDDVVAVAPTAFASHVTTLAGLVVPKPEQLSYAQAAALPIAFMTAVHGLRHV
                     ARLREGERCLIHAAAGGVGMAAVQVAQAAGAEVFATAGSDEKRDLLRSMGVEHVMDSR
                     SLAFADEVRELTDGEGVDVVLNSLGGEAIPRSLALIRPFGHFVEIGKRDIFQNSRVGL
                     RPFSKAISFHSVDLDRLIRERHSYGGELLHEVMEGFADGTFKPPPVHVERVDQIGRAF
                     RMMAQARHVGKLSISFEGVASDGEASQPKVREDGTYLVTGGHGALGLAVARRLAERGA
                     GAVALVGRRAAPKEVEEAAAEIEAGGTRVILEVADATSEEDLAQVLARLRSEAPEVRG
                     IVHAAGRLEDAFVVNLTAEQTRAVVAPKAIGAWALHRATADLPLDFFVLFSSATAVLG
                     SPGQAHYAAGNAFMNALADRRRQDSQTALSIGWGPWAEIGMAARANEDSRSVVDQAGS
                     IPPDRGLDVLEQLIAEDATRSEVLPFDWEAFGSLFPAMARSPLIADLVGSKSDGREAD
                     PAVAEILAAASDELDGALERFVQLELSRVVEMDPDELDPAIRVNDIGLDSMMALELKN
                     RVEGALEIELPIVELIEGPSITQLAVRLAELIREERGENGAPVPEADPAAAAVVGG"
     gene            complement(11207..11620)
                     /locus_tag="VF517_07095"
     CDS             complement(11207..11620)
                     /locus_tag="VF517_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052742.1"
                     /translation="MKEWKSPVSGQLVKLLQHTRTERGMTLADYQTRCVLRGEIHEIV
                     TTDQRDADAGERIDRVGFVGFAELDRGGVVERGDVALAGGVEFGRVLGFDDCHFPNHL
                     NVLVAANRLLTAHDLGAEVGSEIRFEPAPEGPSPE"
     gene            complement(11617..12552)
                     /locus_tag="VF517_07100"
     CDS             complement(11617..12552)
                     /locus_tag="VF517_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8052743.1"
                     /translation="MALRVAMLGLGVISRYYALAIEELSEVELVAVCDRNDERLEPYR
                     RQGVACDRDWRTTLEREEVEAVLVNLPNDLHAEVCGAALRAGRHVCCEKPLALTEADA
                     DALDAASREHGAALFTAFHRRYNANLADLVERLGDRTVASARARYHEKIEEHAGSDTW
                     YLDPVRCGGGCLADNGPNAIDTLQHVLGPLEPVSAEIVRDERGVDLQAWVELRTAGGV
                     PATVELDWAYEHGELKDVTIETADGETLEADMLAGYEAFKSSLEHEYVGVLRDFAGRV
                     ERGERGDEPGVSVVRLVQRSYALAEPAAAAVEGEA"
     gene            complement(12553..13656)
                     /locus_tag="VF517_07105"
     CDS             complement(12553..13656)
                     /locus_tag="VF517_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX8052744.1"
                     /translation="MSDRPRIEPDALRAFTAAVFGICGMEPGPAAEAARSLCYADAVG
                     MDTHGVANLAGLYVPRLRDGRVDPRARPEVVSERGGAALLDGHDGLGLYVGVVALDEA
                     MTRAREHGVGAVAVRRSSHLGPAGYYSARAAEEGMVAMAMSNCGTQRIARPPGGGEAM
                     LGTNPLSAGAPAGDLPSYVLDMSTTVVPTGRVRTAHASGETIPEGWLVDDDGRPVTDP
                     AALDEGRGHIQWLGGRPETGAYKGFGLGLLVDILCGALPGAGVGPLGEPGADDRDVGH
                     FILALDVSAFRPVADFEATMDAMLGALLGSPPAREGGRVLYPGHVESDSAAERAANGI
                     PVAPHVVSKLDQLATELGIDPPAWLPQGAGTSA"
     gene            complement(13653..14963)
                     /locus_tag="VF517_07110"
     CDS             complement(13653..14963)
                     /locus_tag="VF517_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8052745.1"
                     /translation="MSESRTAPAFDDAHAGLLLELLRTPTVTPMETRMPSAIGDAQLL
                     IAESAAELGFEVELHEPPPAAALDAPGVPTPVREVADAMGRDEFLACQPNMVLRLGPE
                     TDHERTLMLNAHLDTVGGDVPVGERDGVISGRGAVDMKGPAVALLAAVRDALRERPDL
                     TERIAVRLQLVSGEESGAMGSYGTRVLVERGLTGRLNVFAEPTGGVYFDSCTASMTAR
                     VTVRGSGSTDDEPDAGENATLLLGHVAVELAARLDGPATRAGCKLCVGGLTTGTMHDR
                     VYGEGTLMVNIPYPSAEAAETMERELEEAVRGACASFAERFAGAGVAARTAQAAERIC
                     HVEWVKRGLPTLEGRDAEFEGRLAAVGIERLPESRSHERFTCDAIWGSLADGYSIVFG
                     PGTLRKNRAHAAGEFVRREDLESYTRAAARLVLDFDDRAAGLAS"
     gene            complement(14987..15787)
                     /locus_tag="VF517_07115"
     CDS             complement(14987..15787)
                     /locus_tag="VF517_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052746.1"
                     /translation="MPDTDDFGVDPPPRRPLPRPAPSGGGGEQPSALLRAMFALTLFA
                     LLLLTVSTFLLWQVADHTKDGGGDNAPTTQAPPALPPASAPGGGKALLKELDQTTATT
                     KALLEEFRAANLGALTPFIAQLVQNTGGLQNTNVLLLRLLEQTSGLNSLARDIGQLQV
                     TLTSLTRQVPTLGARIGATNRSLGATTRILGATNNTLLGTSDQLGAVSNTLRTTNGSV
                     SSLGQNIVGITSTLQQLKQSLDAAKQSIDHTNECLESPVVCQAEDSQP"
     gene            complement(15801..16580)
                     /locus_tag="VF517_07120"
     CDS             complement(15801..16580)
                     /locus_tag="VF517_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052747.1"
                     /translation="MASLRTTSVILAAAGVMALAGCGGDDKKDEGDELGGGSASLQPP
                     ETPKPEDCAGDALKTGERADNLAPKPGTYTYDQKGTRSESGSGSGTALPKTAELRVTP
                     TIKVGNLVCFRSQTAYSPKEADTTTFVIRGGDVYVTSLDSFVAGQTAQFRPNPPIRGV
                     DGSGAEQWTGSFGGPTQGTYEGTLLPRQSFSFEGKSTKSPGVKLHFAFKGELSGESTQ
                     ETYVSLDDGTLFRQKVDQTRNLGASSVKLKYDWKLKSFSGD"
     gene            complement(16640..17548)
                     /locus_tag="VF517_07125"
     CDS             complement(16640..17548)
                     /locus_tag="VF517_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX8052748.1"
                     /translation="MTGTTGERTSATGAALVHGLGEGEPPAVTLVGGKAASVDRLARL
                     GFATPPGFCLTAAAHRAYRDGAGLTERYDGLRARLPDEEARAELQELARASALPAPLR
                     TALDERVAALRSALPDGARLAVRSSATDEDGVSASFAGAHESVLGVECTAEAVEEAVR
                     TCWASLWSAGAMAYRERRSLGVETEMAVVVQALVEAEGSAVAFTKDPVTGSTRELLVA
                     TCESFGEGIMAGGEDAVTFRVDRGTRALLEVEGASGPWPISLADLNDLVEIVLAVERS
                     YECPVDVEAAFDGEQWTLVQARPVTT"
     gene            complement(17545..18480)
                     /locus_tag="VF517_07130"
     CDS             complement(17545..18480)
                     /locus_tag="VF517_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052749.1"
                     /translation="MSTRVAAVDVGSDSTRLLIADVDGPNAIKPVVRTSIPTQLSAGL
                     YECGQLTVAAQARALKALAQHAAEIEQHSCVTSGAVLTSAVREAQNGAEFTAEVRRRF
                     RINARVIDGHTEAELTYHGATLGRDFDGRVLVVDVGGGSTDLMIGERGTPLFKASTQA
                     GAVRKAARFLHGDPPRYEEVASLRANIQRVLEDAVPAEMRTGLAHGLMSAEGWTWRPA
                     LTALGDEGAEAAESGILSRTLIEQALDRLIGARMAELREAPGIHEARAPTLVAGTAIV
                     METMDAFGLDRLQLAEGDLLEGLAMSLASQGVAVG"
     gene            complement(18477..19358)
                     /locus_tag="VF517_07135"
     CDS             complement(18477..19358)
                     /locus_tag="VF517_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX8052750.1"
                     /translation="MAAEPSAVALAGIGDEAAPDLAGQIAAHRRLGWSHIELRTIDGA
                     QIADMPLDAVSAVAEELRSEGLEVVGVSSGIGAWGRTTGTPLEVDERELEALAPRAAA
                     LGCTRLRVMSFLQEDVDDAGWRAESRRRLGELARRAEQLGLTLLHENCAGYGGRGPEE
                     SLELMAGVDSPALRLLFDTGNGLSYGYDSLDFLRPTLDWTEHVHVKDAVRRGDDVEYV
                     PPGEGDGQVEECLALLLSSGYRGGFTIEPHMHVAPHRGERLAGDGATASFVECGRALE
                     RLMERVAEPARGGAAAG"
     gene            complement(19348..20475)
                     /locus_tag="VF517_07140"
     CDS             complement(19348..20475)
                     /locus_tag="VF517_07140"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8052751.1"
                     /translation="MILDSPSVPYYGMDRRTHPDRAFIEEALLDVARSDEFILKRRVA
                     AFEEELCRYSGAGGAVAVASGTHALEVALTALGVGPGVEVIVPAFGFHSSASAVARRG
                     GKPVLVDVDPETWHLDPDRLADAIGESTAGVVPVHLFTQMADMPAIVALAREHDAWVL
                     ENSAVAIGMRRDGRMAGLFGDIGLYSFQPVKPIGGASDGAAIVGDDEELIRRCRMLRN
                     HGQDGITRFLHHIVGFNARMDEVTGALLSRRLARYDESIARRAEIAARYDEALADLAP
                     DLRVPPPADHEQVFYTYTVACRDRDGLERHLAERGIETKVYYPLPLHLQPAFAWLGHG
                     EGDFPNAERACRESLALPLYPDMPDADVEATIAAVLEHHGR"
     gene            complement(20472..21629)
                     /locus_tag="VF517_07145"
     CDS             complement(20472..21629)
                     /locus_tag="VF517_07145"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8052752.1"
                     /translation="MAVPFFSGGRSLERRWSRVRELLDEAFDSGTFVNGPVVGRFERA
                     MEEYTGARHAIGVASGTDALIVALEACGIGPGDEVAVPVYTFFASASSVVHVGARPVF
                     VDVKPQSYAIDPADLEKRLTPNTKAVMPVHLFTQMADMRAISDVADRHGLTVVEDSAE
                     AIGMDFDGTPGGRFGRAGVLSFFPTKTLGALGDAGMVLTDDDDVAELARRLRNHGQVP
                     GERPYVWDVMGMNSRMDDVQAAVLLTRLETLDADIARRAEIAARYDERLSGVVQTPAI
                     VDERATAVYYVYLIEADDRDALVEHLTAAGIETEVYYPRPLHLQPCFADMGHGPGDFP
                     VAEKAAGRAVGLPLYPDMDDAQVDEVCAAIAGFYESGGGPGRAVEHAEGVR"
     gene            complement(21629..22678)
                     /locus_tag="VF517_07150"
     CDS             complement(21629..22678)
                     /locus_tag="VF517_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8052753.1"
                     /translation="MLSTLILGFGNSGRALHLRSLLHAHRLCKGEPVFATSPVVAVDP
                     AMYAKARARGDGSVVLVRSLDQVEGIEPADTVAHVCTPPATRVAVLRQLSARGYRRIV
                     CEKPVAGSVEDLHEILEIVEQEELDVAVVSPWLSSTLTLSLREAVDLATFGRLKTIKI
                     EQLKPRFTRTRDKVDHPTAFEIEVPHSMGVALHLGGTQAEVVSAGSSDMVLDGVAYPH
                     LGRASVELSHRGGVSTVIDCDLTAPVRARSIELGFELGTVLGHYPVGDDTYAQLQVMP
                     DGGPPRERELLLDDQLAKMMVDWYTHYADLGPRPVSDLRFNVLVTDTIAAAKRAAGIE
                     EPGRIVDEPLAEVPG"
     gene            complement(22689..22925)
                     /locus_tag="VF517_07155"
     CDS             complement(22689..22925)
                     /locus_tag="VF517_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8052754.1"
                     /translation="MEFERYYQELKGTSRAVRPEDRLDLLGIDSLLAQELLASLEDTY
                     GIDLMQDERLMKVRTVDDLLDVLEEHMQKATESA"
     gene            complement(22942..24462)
                     /locus_tag="VF517_07160"
     CDS             complement(22942..24462)
                     /locus_tag="VF517_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX8052755.1"
                     /translation="MTEGRLSIDTEVHPRFPVYSAGNFGEVAPERLSIAAWSLIGDPV
                     ERGCRALAQMLWPRATWYTGSLYVFVGYFRCRPYHNLSAFCHMGCEVPGTSVEDVIGS
                     YFEDAPEPERPKGVGAGTLGRAGGVWRIAREGVSLRGALTESDGRVHLLEEEARRAVD
                     SGSEFALGACWERATALLPEIWKIHYTTTLGLVPLRAAQRGIGRRALGYWPELEPWLN
                     RPQELVWSMLHDASQDPTPLAAGEFLDSAFYEVADHHEPWATYATRHGGGQLAEPPAE
                     RDDLREVAWGILPRSRRALLPQLDRLVSDTMSSRENSKSQAMRCMHVFRRLLPLAAES
                     AGVAHEDWPYLTVGELDGSLSVAEASERAARRRAECERGLAQEAPELVAPGRRGGAPI
                     EAPGREKRFGKGRGVSPGLATGLVVSPSNPQLDGELNGARRILVCDSADADIQPLLPQ
                     MSGVITARGSALSHVSILAREYGVPAVVGHALARELQPGQEVSLDGTTGEVTVIGD"
     gene            complement(24459..25436)
                     /locus_tag="VF517_07165"
     CDS             complement(24459..25436)
                     /locus_tag="VF517_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052756.1"
                     /translation="MRVLVVGGTGFVGAEVVARLAAVGVDVVSLSRSGTAVAGEGVSG
                     DARAPDMGLRGDALRDLLESVTHVVSSFGTVDWDSGPRIVELHEAGTRNVLDLAARMP
                     NLERLVHVSSVLALGRAEGVVGNADLDVGQTFRSWYDYAKFTAERRVRLEESLPWRVL
                     RLGPVLGVGAAGAPSAHAGILAALPPLVRGYPVHLTDRGAFPCYPTDAAAAAEVTRRA
                     LFEPGDHDAWTWFDPDSPTLGQVFVGLCAAWNVLPRIVEARAWGGASQLLARRFGAPD
                     VLLSYSEPWVDLAPDVLEAIPGELPRCEPGYLEATSAALHARQSELVPA"
     gene            complement(25433..26551)
                     /locus_tag="VF517_07170"
     CDS             complement(25433..26551)
                     /locus_tag="VF517_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052757.1"
                     /translation="MRILVAGITGQLGHGLVEVAADHDVELVPLTRRVGRRDAKARLG
                     RMFRDAPELAEGAIEGDVTAPYWGLSAPRVRRLAGTVDAVLDIAGETNWAAPTRRLHA
                     VNVLGALNGQQLAGAIGEHGDAPLFCYASSIHAAGAVRGQVAELPFPASGERTAYEQS
                     KWLAERRLIDAGRSVPTAIARIGGLVGNSVTGATAKRNSLYMLADRWESMPGGLMPYV
                     RKGRVDMLPRDDAARLLLGMIGRLRGQPPGESAIAHVCAGEAAPTTDALLAAIDYVAE
                     GRVPSRRRVPVPARALVWSSNNLDRLRSFPAAEQNLLVGLRYLALDRVFERARMASLV
                     DWPLPKPSAEQLARLAFESEAAEPPPLQGDLALARFAG"
     gene            complement(26548..27780)
                     /locus_tag="VF517_07175"
     CDS             complement(26548..27780)
                     /locus_tag="VF517_07175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8052758.1"
                     /translation="MSATATERPLLLSGGIDSEEVRAYRELVAERVRSVAPLVPDAEE
                     SGRFPRAAIEAIGGEGLARERWTGGPHGDAGKGVVISEELGRAGLGGVGVGVSVHLDV
                     VLAALTRFGKSDLLRQRTEAALDCTEIGCFAATEDGEGSDLLGMRTTATRTDAGWHVK
                     GTKAFASLGAVADFALVLCRSGDGSAAAERSAGSAIGPPLSVVCVPSDGFRVVRRLKP
                     LGMRGLETVRIEIDAEVPDEAVLGREGRGFLITTWALTHERLASVAQLIGGADLGLSL
                     ATTRLHRRRQFGTPLIEHQTLRLRLAEIAAQATTGRLALYALAASLDRLRPEWAREVA
                     ALKVTLARIAERASSECLNMFGGLGYLEDATPLARFFRDGRFARLGGGTDEIMLELVA
                     AGLPVNDELYDAHVQEMA"
     gene            complement(27777..29501)
                     /locus_tag="VF517_07180"
     CDS             complement(27777..29501)
                     /locus_tag="VF517_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8052759.1"
                     /translation="MSRLLGWLESPETAFGMHFATDDGGWEYYDWGEMAARVHDAAAK
                     IAADRRERDAPIAIILPNGPDFEACFFGALATGNFACPLAPPNLLQDPDGYANHIAAL
                     VKAADPALIVTAPRYLELIERAVQIADRVDRCPVRAFDLEPTSSEFAVEKPAELALLQ
                     FTSGSSGRPRGTRISWSNLEANIGIMEEWLGGDRYSDQIATWLPHYHDMGLIGAMLTP
                     AVYQGHVWVMRPEQFVRRPIRWLECFGKRGATMGASPTFGYAHILRRVKPHQLDGMNF
                     SGWRAAVSGAERVDAAVFSQFGELLEPFGFRASSLIAGYGLAEATLLVCGRSHQKTGT
                     AVKLDWSQLELGKPVVVTDRAMLGDSASVGDGSGWLVGCGSTHRGVSLTVLDEDGTDL
                     PPGHLGELFVTGRCVAGGYEGGDEVNTAKFVNGGVRTGDAGFVYDGELYVVGRIGDSI
                     KIRGRALYAEDLEARLALVEGIGIGRCAVFTGSGHHGDTVVAVVEGPTAEWADTALQI
                     LADETGGAVRVELLRGRAGTIARTSSGKVRRRVMWRIYAEEGIDAEVVGSITPEGAPA
                     ARPSEVGA"
     gene            complement(29698..30674)
                     /locus_tag="VF517_07185"
                     /pseudo
     CDS             complement(29698..30674)
                     /locus_tag="VF517_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(30759..31952)
                     /locus_tag="VF517_07190"
     CDS             complement(30759..31952)
                     /locus_tag="VF517_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX8052760.1"
                     /translation="MSGGANIRVVGGGLAGLVASIASAEKGCTVELFEAGPALGGRAR
                     TRDGDFRANLGAHVLYADGRSWPWLEERDLLPPYSRSPKRGLRFVHKGSLKRVPPSEF
                     LQGLRLREKRAPSDLSFAEWAAREVGEDAVSVLANSASVFTFDHDPGRLAAEFIRQRC
                     ARVLWSVPQNRYLTGGWQALVDSLERRARELGVAIHTDEQVEELPEPPVIVATEVRRA
                     RKLLGEELPVDTTRCVLLDLGLREQKGDPFIVFDLDGGGWLERYTAADETLAPEGHDL
                     VQGHLGIRPGESPDATTERLEALADIGFEGWRERTVWRRRLVVDGMTGALDLPGRTWR
                     DRPSIDRGGGVFLAGDYTAARGLLSEVAFASAIEAAEGAVAVASGAGATAAAGSASAA
                     ARARG"
     gene            31980..33440
                     /locus_tag="VF517_07195"
     CDS             31980..33440
                     /locus_tag="VF517_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8052761.1"
                     /translation="MNFAEILTASAAAHPDRPAVCLGPERVTYAELYDRALRIASALR
                     ERGHEAGSVVAVMLQNGPEFVAAYFGVLLAGSVVTAVNPLLTPGEVDRLLENAGARTL
                     IGGDPERPPAPGVTRIDPADAAAAEPLKEPVPRADDDTALLLYSSGTTSGPKAAEMTH
                     HNLVWSSDAMVAAVGAPSAALCVAPFFQGFGQGCGMNGMLRAGATIHTQERWNAAAAL
                     DTLEHERVPLLQAVPAMCHDLLELPDVHERDRSALQLCMTGGAATPLDLLKRFEHEFD
                     CEVLEGYGVTEALRVSMNRPGRRRAGSIGVLLEGVEHRFVDSAGDDVVDGAGELVIRG
                     PNVMRGYWGQPEATAEVLRDGWLYTRDLSYEDEDGFLWVIGRVDDLISRGGYKVHPVE
                     VERLLAEHPAVAEAAVIALPDERLGSEIGAAVVVAEGHELDTEELREFMRERVAKWKY
                     PRRIWAVPSLPRGITGKLLRREVAPPTPDEDPAASR"
     gene            complement(33463..34734)
                     /locus_tag="VF517_07200"
     CDS             complement(33463..34734)
                     /locus_tag="VF517_07200"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX8052762.1"
                     /translation="MAVAHSTVADLCLAAREASHSVVAVSAHARTRAILAMAERLEAP
                     GAADALVAASEADAEDQRQAGASAFREPQLDARRVRALADYCREAAELPDPVADPIEV
                     RELANGLRVEERRMPLGVVAWAFHGRPHTAIEAAAIALRAGNATVLKGTAPTRRANAQ
                     LAELASAAVESAGLPAATVTFVPATDDDSLRELATQHATVALLKQRGGHPLHRLLRGV
                     ASVPVVYAIEGCCHLYVHADADPELATAVAVDSKASRPGRCESIETMLVHEGALEGFL
                     PRALSELHGRGVELRCDTRAIDAARAAGVPADAATDDDWAAEYQSPILAVGVVDSLDE
                     AVEHVTRYGSGHAEAIATGSGETARAFTQRVDAAYVYVNASTRFSDGRGLGFGPDFGN
                     SVQKLPVRGPIRVRDLTTRQFVVEGDGQVRG"
     gene            34823..35986
                     /locus_tag="VF517_07205"
     CDS             34823..35986
                     /locus_tag="VF517_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8052763.1"
                     /translation="MSTEQADVAVVGAGVAGAAAACALARAGLRTVVYERRHLDRDAN
                     RGDALHADAVARVESWDALPALENRGAFWVRRSVFTTPRGAPRLEMPLNGGALLMLAH
                     ADIELGLAETAEQYGAEVRDQAVKEAHRDGDGWRVVTKTGEELRVRLLVGADGARSLV
                     REAASIPVESHQFGQSTVVLHAPLPDWLDDECGFATIHPDGGVLILPTTPRGLCRVVA
                     QVDDEDLPRWRSADPTELRELLGARDSRLGELDVQRYTGSHVYRLGWQHAQRYVAPGL
                     ALVGDAAHVTHPNGGQGMTLAVYDAAALGQVVAPALLNGNGNGDGRSLTAALWEYAAR
                     RRMPSWESLERANRVARFQRKGTLPYAGSVAMLGLFSISPSVTARLARRFGGE"
     gene            35983..36810
                     /locus_tag="VF517_07210"
     CDS             35983..36810
                     /locus_tag="VF517_07210"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052764.1"
                     /translation="MSDRRVAIVTGASRGIGEATAWRLAAAGWHVFATARDTASLEGM
                     ASERVTPLRLDLNDDDSIQDCVNTVLTDAGRVDALVSNAGYSASGALELFELEDVRRQ
                     FQTNVFGPLRMSQLVLPAMRAQGSGRIVHLGTVMGRVSFPLLGAYSSSKAALESLNDA
                     LRLEARRFGVRVVMVIPGTVRTEFDQVALDTLAERMPDEGSPHRESLEKLEKMISDTS
                     EKGIPPEAVAAKVQEALEARHPRSVYVLTPEARLAMRVLARLPVAVRDPLMRMPLRL"
     gene            complement(36948..38585)
                     /locus_tag="VF517_07215"
     CDS             complement(36948..38585)
                     /locus_tag="VF517_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8052765.1"
                     /translation="MRRLAGAGARAKGPFSRGIATAWEYAAERYPDQRTAGDRPPDID
                     PDRDIATFSDQAQFVREVASWLREGGVESGDRVAIIKANHSDIVALSCAAARIGAVPA
                     QIACTHSPEVARELLARLDRPVLVTDRARMESCGLGSDEVAKLVKRIIVVDAEPGSNG
                     STTALADLRGGSIPPLTPRPDDEAMVITHTSGTTGTPKLVAHSAATLRALSAVESKRW
                     PVLGLKGGDSVLFCDPFVHGRVFTGLAAVTKNGPRLTMLSDPVAPNVRDLLVEHRPTV
                     IETVPNAFLLWEAMADDPGEPFRDVRLFINTFDAVNVRSVRTLLNASKRRFPLWVQGW
                     GQTEAGPVTLGIYTRRSVRDRGGRMPATRKVGRPVPFITKVRAVDPDTGRPLGRGRAG
                     LIEVHKRGCALGYVGEDDRFQSKRNDGWWNMGDLGVVSRTNEVQLLDREVDRIDEGSC
                     LELEDVLLDRMPSLVEAVMLAVNGGPPLPVLNMADGQPLDPDAWKRAVADLPELADPV
                     VLPWSDVPRTATWKVQRQKLREQVAPGTEPVGSGRWT"
     gene            38670..39665
                     /locus_tag="VF517_07220"
     CDS             38670..39665
                     /locus_tag="VF517_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX8052766.1"
                     /translation="MAEVGEAARGADVLDVGRDGPSAGPTTHGFKERLIAYKDLAKLE
                     IFDVYLTVPLAASLLTADQFGTAESIAILVLMLFTQIGVVTAACALDDVAGIRDGIDT
                     YTYGQDSNLRPLKRKPLVNNRLTEAQAIRYAYIAAGVGVACVAAAYAVADFEPRWAPL
                     MVVAIVTCVLFYSWGPKLSYIGGQEFVVVLGVTFTLAGTYALLTGGMTWTVVLEGLLL
                     GIWLMQLTAFANQHDREGDKRANRVTMAVRLDPSTYNRYLTTLYVLSLVTLAIGLVTE
                     ELPWELAPLAIPALVIQWIAFNAGVMRGDGLRARALSLTVLRAGWVALFVANLVA"
     gene            39675..41876
                     /locus_tag="VF517_07225"
     CDS             39675..41876
                     /locus_tag="VF517_07225"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8052767.1"
                     /translation="MGDLLLKLDGWLTRRRKLVIGLWVAALVVAAPLSAIQSTALTEG
                     GFQVEGSDSHTANQALQDDFGERQPNALGVVLVREGASPQEFSKSVDEYAKIAAGVDN
                     IEVPPAAVESARTRALTSDKVLFALTVIGNQDESADAAVKLRDDLKVKEGARDGVRPY
                     MIGAMAFWAALHELQKTDVQKAERVGVPITALILLATFGTLAAAALPLLLGIASVVIT
                     GALIFLVAQALPMSVYVTSSASMIGIGVAIDYSLFVLARYRQEIASGRTEDDARREAL
                     RTSGIAVVFSGLTVIVSLCALLAFPTVALRSMALGAIIVVSMAMLAAVTLMPAVMTIL
                     GPRVYEPGRTAVWRDRIVGRFRREGGDFWGTWTRRVMARPMVTAILAALALLALAIPA
                     LDVEIREESRSQLPAGHDAIQGLNQAAPLIGPGALGPFVAVVRFNSGTAKTPANQQAL
                     SAYSSAVESDREVAYVTPPRPSEDGRQAALTIVPRHFGEHPDAQALLGRLRADRGGRA
                     LAPVGSVSFGGDVAMVHDFADNVADGVFRAFLILVCVTFVIMMILLRSMLLPVKAVVM
                     NILTVAATTGIIVGIFQWGWLSWTGYEEPGYIAAEVIPLMMAVVFGLSMDYEVFMLTR
                     IREAFMRSGDNREAVANGLRSSASTITSAALIMVSVFFAFAAVRIPSIRQIGTALGVA
                     VALDATLVRLVLVPATMGLLGRWNWWLPTWLHRMPRVLPEVVPESGVSKGS"
     gene            41879..42685
                     /locus_tag="VF517_07230"
     CDS             41879..42685
                     /locus_tag="VF517_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8052768.1"
                     /translation="MAVGTEGQGSYALFGNVGDEQLRRLKSSSAVWDEGTLRFLMGLG
                     LATGWRCWEVGAGEGSVARAMAQVVGPTGHVVATDYDPHFLVSSAAQNLTVIEHDVVT
                     EDPPGEGFDLVHARMLLQHLPDREEAFRRMAESVAPGGWLALEDSDWITFLVARPAIP
                     ELRVLASALDAAMADAGFDSTCGFGNVTRLNDSGFEDVRGQGHVLVMRGRSTGVTWYH
                     QWAASLRDSMVAAGYLTDDEFERADAVLDDPSAAWLSQTLVAACGRKPSK"
     gene            42697..43512
                     /locus_tag="VF517_07235"
     CDS             42697..43512
                     /locus_tag="VF517_07235"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8052769.1"
                     /translation="MATPEETPDQGAYALLTEVEEVDQARMLLSAASVWDQGTLRRMC
                     DLGMTEGWRCWEVGAGEGSTGRAMARVVGESGHVVVSDIDTTLLDTAGLANVEAVQHD
                     VVGDDPPGGPFDLVHARCLLPHLPEREEAFARLASAVAPGGWLMLEDTDWATFLVAHP
                     PMREFELLRAGLEAAMAEGGFDNTCGMGNFHRLRGADFEDVRGEAEASVMRAGSPGVT
                     WYHHWSGRLREQMVAAGHITDAEFELANARFDDPDAVWLSQTMISASGRRPLE"
     gene            complement(43518..45137)
                     /locus_tag="VF517_07240"
     CDS             complement(43518..45137)
                     /locus_tag="VF517_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8052770.1"
                     /translation="MLGRTRDARMARLGTLLEDAAERVGDQQVIDTRASDVDPARPLE
                     GTYASWAEFVRDLAGRLHEAGVRPWDRVAIFKGNNSDVVALACAAARLGAIPAPLAWT
                     HPPDVLATMLSRLDRPVLIADRERLRAGELDAERLRALTSRTLLVEDEVDDLGDDVIA
                     LPSLKGASAPPVQVRADDDPMVIMHTSGTTGVPKLVVHSARTVATAARYETRRVPVLG
                     LRREDTVAFADPFFHGRLTTGLVGAARSGPKLVLVADPDPTAVRDVLVKHAPTIVESA
                     PNAFLSWEPLAADPAGPFRNVRLYFNTFDPIHTRTVRVLLNASGRRFPVWVQALGQSE
                     SGPVSMGLFTRRSVRPGRGRRVAEARGIPVSPLTRVRAVDPDTGRPVGRGNVGLLEVR
                     RDPCLDYVGEHERYLAKRRDGWWNLGDLGIVNRSGTIRLIDREVDRIADASCIQVEST
                     LLERLERATEVIVLAVRDGRPVPVVSTADDEPIDDAAWSAATAGMPPLADPVFIRWDE
                     FPRTATWKVRRHVLREHLLPGTATAGAGRWT"
     gene            complement(45137..46303)
                     /locus_tag="VF517_07245"
     CDS             complement(45137..46303)
                     /locus_tag="VF517_07245"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8052771.1"
                     /translation="MSAGLSVCVTGAAGFVGAHVVRLMVERGHEVRGTYRGGGEDRLR
                     ELGAEPVRADVLDRDAIDAAVRGTGLIVHCAGAVGSPPSGSAWEANTAGPARVVEAAA
                     RAGIERVVVTSSVLALGFAPPGRTGDEEDRVESRVPLVYPESKREGDRGALEAGRRLG
                     VEVVVVHPGYVLGPPVRPEDSGGSSRVVCQYLRGRLPAVVGGDTNLVDVRDVAEGHVL
                     AAERGRPGERYVLGGHDIAWADLIERLAERSGVRHPVFVLPPAFATAARAGERARLPM
                     PMPAETLTLMGGNTRFSSRKAERDLGYRARPLDETLGDTVDWCTERIRAGMDASESSA
                     LSRMSAMIQRAGAAGALGPVHAAERRFGRRLIAGSDGASRTRPRRRRRRAGAAR"
     gene            complement(46349..47269)
                     /locus_tag="VF517_07250"
     CDS             complement(46349..47269)
                     /locus_tag="VF517_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX8052772.1"
                     /translation="MPLTERLLAYKDLAKLTEWGAFLSVPLVWSALEPDHRTSGYALG
                     LLALSLVTQVGFRTVESALDDVAGVRDGIDVVNYAAPSEHRVRARKPLLDGRLTEQQA
                     KGYAYFAATVATIAFVAGLAIADFEPLWVPFAAAGAAFLVVTYSAGPKLSYRGGQEIV
                     VISGLAVAIYGTYAVIEGSLSGAVATEGLLLGIWLMQPVTFANLHDLEGDRQAGRATM
                     AVRLSAEGNRRYILGLFALGWTVLLTGIVLGALDWWVVLFLVPCFAIQHRALRDGLWR
                     GEPLLARRLCHLAYRVGWLGLFCANLVALG"
     gene            complement(47256..48839)
                     /locus_tag="VF517_07255"
     CDS             complement(47256..48839)
                     /locus_tag="VF517_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8052773.1"
                     /translation="MVLARAGMLPLARPDHLLQMLRAMRAFGQIGAISRIGALRHGSA
                     IALDDELGQLDYAALHTRSNSLARALGGLGVRQDDGVAILCRNHRFILDAAFASAKHG
                     ARAIFLNTDSAGPQLAEILEREGATTLVYDDEFAERTADARRGRQGVRAWTELPEGPP
                     GEPTIEELIASADGSELPAPKRPGRVVILTSGTSGTPKGANRGQPKTLTVPAALLSRI
                     PLRAGGVTYVAPPLFHSWGLLTASLTLGLGTRLVLRRRFDAAAVLDTLERTRCTTLVV
                     VPVMLRRLLALGEEEVRGRDLAALRVVAVGGSPLSAELCAEASGVLGDVLYNVYGSTE
                     VAQATIADPDDLRAAPGCVGRPPLGTSVKVLDDAGREAPAGTTGRIFVSNAVQFEGYT
                     GGGGKEVIDGMMATGDLGHIDAGGRLLIDGRDDEMVISGAEKVFPAEVEEVLARHPSV
                     EEAAVTGVPDEEFGQRLRAFVVARPGAGLTEDHVRDHVRENLARYKVPRDVVFMERLP
                     RNATGKVLKRELTGDRAAD"
     gene            48985..50721
                     /locus_tag="VF517_07260"
     CDS             48985..50721
                     /locus_tag="VF517_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8052774.1"
                     /translation="MSVAPRTPRTRPLNRLRLVLDPALTPANALDKACDAHPDRAIFH
                     PDADSGFAALRGRAITPRQLLVFAGGLGELLRDGMGMRAGDRVAVIKTNHVDYFFLVL
                     AIVRAGGVAVPINPGIRGERLERYLRHTGARIVLTDAPTFASCVGDPERMPSVERWGF
                     PAAPAGFGASHVSIDSELPAAGAPAPPAPLRRVDPVVIAHTSGTTGFPKGVVSTSGTL
                     AAGIKGHYVDEPVATGNRTGIAGHFNHLVYQSGLFATLLSSMPVWTLSPDDAAGALRA
                     IERERLNFFFAFPDVYLRMYHEGLAGHDLRSMRLWVTTADASHDVHMRAFCRTGAYLR
                     VLGVPLMRSAFIEVLGSTEVGSAALRRIRLPFARPRSDRPIGRRTPGGPRVRIAREDG
                     RTVWRPGAVARVEVRGPAVFAGYWDEAAMRPAPRERAWAWTGDVGYRDRLGVFHHLDR
                     AADVVDTRAGPVYTLPVEEVLLEHPAVGEVAVIGLPHPDGGELPVAVAHARPGATLDA
                     GECLRWARTQLTLAAPLAEVVVVTPEEMPRGLTGKVLKRELRERYADRLAAPAGAAAG
                     ELADQAGAAAQE"
     gene            complement(50686..52023)
                     /locus_tag="VF517_07265"
     CDS             complement(50686..52023)
                     /locus_tag="VF517_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8052775.1"
                     /translation="MRDRLLGAYRSHYNPSLARMFDAYGAPVELAGSGAVLQDEEGCE
                     YLDFSGGHGVFGVGHGNGAVRAAVLDQLHTLASAPPLLRTAPAATLIGRLAELLPGDL
                     DRIALAGSGSEAMEIALRTARLARPDRRRLVAASDSYHGKTLGALAVMGQEHLRVPFG
                     RSWPSTSFVPHGDAAAAAEAIGDGVLAMVVEPILGGGTLSVPPDGYLAALREHCDATG
                     TLLVVDEVQTGFGRTGTMFAVERDGIVPDMIVLSKAITGGHTPIAVTAVREGLLAGSD
                     ALAAAAAERPSESAGSPLVCAAANAALDFIVEHDLPGRAATLGPVVLDGLERIARSHP
                     ELVHDARGRGLMVGLRLRNRVVENALWLQLLKRGVLGALSLSVARTPVLRLYPPLTVE
                     RDEIERVLDVLDESLGELRRRLRPAVYDLGDQSLRFQHRLPPSLLRRGARLIG"
     gene            complement(52023..53273)
                     /locus_tag="VF517_07270"
     CDS             complement(52023..53273)
                     /locus_tag="VF517_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8052776.1"
                     /translation="MKPAYVEHAAPWGALRREALDAGVAVSAAGATVRTSDGRELVDL
                     SSGGFGYGDRRVLECVEAQMREMPLSSRHFLSRPLATLVERMARVAPGTLSVTYPCNS
                     GSEAVEGALKLARGYRPGRTRVVAMSGAYHGSTLGARAVSDAATVCATIADAPVQVAR
                     VPYDDLDAAAHAIDRRTAAVIVEPVATGAGVRVPRHGYLTGLRSMATRAGALLIVDEV
                     TTGLGRTGCRFAVEREGVAPDVLVVGGALGGGALPVAAYLTTRHVNQRVYGRRDPVLH
                     ASTTGGSPVACAAALAVLDAVEEEQLERACAGHGARLMEQLERWRDERPQLVLDPDGC
                     GLLAGFRVRDPDLAYELQHAAAAAGALVGAGATGTGAGWIALRPPLAIGAEELERGLA
                     ALERALRSVEDADAGAAETAAVRA"
     gene            complement(53343..53882)
                     /locus_tag="VF517_07275"
     CDS             complement(53343..53882)
                     /locus_tag="VF517_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052777.1"
                     /translation="MTSIGDPPPDRPAAIGDEPGTAREGTDPAEKSAAYERRAAARRA
                     SFADREARRRSSFESRERRRRAGYDSCEATRQTLVATGEFYRSLGAAVADSFERFNAE
                     LEPEAVDAQGLGRSTFEAFAEGNAEFYESLADSSRRVFDELRPDPDRPVYGGRYEPID
                     YELLARLVADELRRSGTTT"
     gene            complement(53902..55734)
                     /locus_tag="VF517_07280"
     CDS             complement(53902..55734)
                     /locus_tag="VF517_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX8052778.1"
                     /translation="MEKLTDLRTPPARDEPQLAAPAGVPDLDGFAEDPGPRPRATRRR
                     PRQLPLPLLFVARSIRIVAFSTAFAVAFALHRLVSPSSGPRLVRWYLQSTGAAFVKLG
                     QILAMRYDLLPARYCEELSKLLDRLPPVPFETVVQVIEDDLQVPIEDCFGELEPEALA
                     AASIAQVHAARLHNGQSVVVKVIRPGTPQRFRVDFVYMRLGARLLGGLGAFGELDLRR
                     LVRELIRLTREELDFRREARNVWEMHEAMLADGVDHYSPALVPELCGRRVLTMERLEG
                     VSVAALIAALETGDRDALARFAEMGISPERTAELVMRSILEQSMRHRIFHADPHAANL
                     IVMPGGTLGWIDFGMLGWLDERLWRAQFGMRHAVAEGNVYEAYQHLLDTLEPLPPIDL
                     STFEAEIIELFRDWTSSSQAPTASLLEKSSGLVFLRIFDAVRRAGLRLPTGLVRLYRS
                     IFVADIVMLKLHPGIDWVPIVRRFVADELWRQSGERLGRITSPATINAAVRATVDAPR
                     TLAALSDWVESGLPGVGREYRQQRDRAERIGGLLFSYARAAVALATLALIGSVTVAPL
                     LFEGGGWDRLEEGLGPPLWLVVAFGTFATLLLGRLAREFRRGAR"
     gene            complement(55823..57997)
                     /locus_tag="VF517_07285"
     CDS             complement(55823..57997)
                     /locus_tag="VF517_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEX8052779.1"
                     /translation="MLHAAEPIDEAEIVVVGAGPAGSSCAAHLAERGHEVLLVDQSAF
                     PRDKPCGDGLTRSAVAGLDRLGLPDLFGGRPPIEGLRMVFGYRSSEYRRYDPSPGGHQ
                     AYCVERRVLDSALLDLARARGARFLEARATSMVAGEEQTVLVTRRAEPRDVALRARFV
                     VAADGSTSRLARQIGQIGRSRRSETAGAYAVRAYYRCEHEVAPVFNAYMPLELGDLRF
                     PGYGWVFPIDRHTANIGVGYWRADGVSSPKRIRDVLAAFVDQLRTRERSRYGDIEERS
                     KLFGAPLGVQFDPGRCEAPGVVLIGDAARTTDPVSGEGIAYALHGAEMIAELVHERSR
                     NGRAGLGAGRALARRFPRLGQDVGLPLRMADRRRHQVDDYRQKTSAHPFVRTMRNVVT
                     SPEDQPSLTETVAGGVLRGGPGAEWLERLNHLVLDTVATSFPVAAELLHSEVRRGLGP
                     VLSVAAALTRDGGGHDDPRSMSAAAAAELVRAGTNVAREVNDRPRTRGGQANNAMCLL
                     VGDFALSRASRHAAAAGSGFAAATGRAIQAICDAQFVESRSLFDLARAPEAVVETARA
                     RMSTLLELSLRFAGESAGFSAAAIADLVAYGRELGVAVRLAEDLAVLLDGDEPTNRPA
                     GTELSLGAYPLPVLWAAADDAALGRLLSGDDVLDRHDEVIARASATGAIERAADQVAL
                     YAERARAALPAGARPGLRDLADAVEAHARALVASTAGAVQHA"
BASE COUNT         7556 a        21642 c        21207 g         7686 t
ORIGIN      
        1 cacggagccg ccgcggcggc gtgccgcggc gccgaggtag gccgtcgcct tgccgtagcc
       61 gcacgcgatc tcgacgagct gcggcgcgtc gcgggcggac gcgaggtcga acagcaggtg
      121 gcaggccggc gtatgcgtgt agggcatgtc gccgaggagg gagtcgaggc ggcgcagcgt
      181 ctcggcgcgg ctcgtggctt cgcgcatcgg cttcagcgta gaatcgcgcg ctgggcaggc
      241 tgcgcaaccc gggggtggcg gtgaccgagt acagacgtcg cgggcgctgg atcgaccact
      301 gggacccgga ggacgagcgg ttctgggaga gcaccggcag gcgcatcgcc cggaagaacc
      361 tggcgctgtc gatcttcgcc gagcacctcg gcttcagcat ctgggtgctg tggacgatca
      421 tcgtcatcaa cctcggcaac gcggggatca ccctctcgct ggctgagcag ttctggctga
      481 cggcggtgcc gaacctgatc ggctcgacgc tgcggctccc gtacaccttc gcggtgccgc
      541 ggttcggcgg gcgggcgtgg accaccttca gcgccgggct gctgctcatc cccgccctgc
      601 tcgtggcgat cgtcgtgccg agcggatggc tggccgacca gtcgcacagc acgcagttct
      661 gggtgctcgt cgcctgcgcc gccacggccg gagtcggcgg cggcaacttc tcctcctcga
      721 tggccaacat ctcgttcttc tatcccgagg gcaggaaggg cttcgcgctc ggcctgaacg
      781 cggccggcgg caacctcggc gtggcgctca cgcagctcct ggtgccgctg gcgatcatcg
      841 tgggagtgcc cgccgcggcc gtgaagctgc ccgcccacga cgtgcacctc gcgtacgcgg
      901 gcctggtgtg gctcccgctg atcgtgatcg cggcgctcgg ggcgtggctc tacatggaca
      961 gcctgagcca ggcgcgctcg gaccagcgct cctacctgct ggccttccgc caggggcaga
     1021 cgtggatcat gtcgctcctc tacatcggca cgttcggatc gttcatcggc tactcgttcg
     1081 cgctgccgct ggtgatcaag aacaccttcc cggagttcct cgccgaccac ccgttcatcg
     1141 ccacctacct cgccggcttc gcgttcctcg gggcgctggt cgggtcggtc gcgcggccgg
     1201 tcggcggctg gctgtcggac cgcgtgggcg gggcgcgtgt cacgctcggg gtcttcctcg
     1261 ggatgagcgt gctcaccatg ctcgcgatcg tcggcgtgcg cgagcacagc ttcccgctgt
     1321 tcttctcgtc gtacatggtg atcttcctgc tggcggggat gggcaacggg tcgacctacc
     1381 ggatgatccc cgcgatcttc gacgcgctcg gccgccgcga ggccgaggag aacggctaca
     1441 gcggcgagcg gctgcgcgag atgctcctga ggtacaagcg ccaggccgcc gcggccatcg
     1501 gcatcgcggg ggcgatcggc gcgtttggcg gcttcatgat ccagatggtg ttccgccagg
     1561 cgagcctcga ggtctcggcg ctggtcaagt ccgccgacgg tgtggccgcc aaggccgccg
     1621 tcgcgcaggc gcacgccgag tggtcggtcc cggcgctggg ggcgttcctc ggcgcctacg
     1681 tcctcttcgc cgggatgacc tggttcttct acctgcgcag gtcgttcgcg gtggagaagg
     1741 tgccgagcct ggcctcggcg tcggtctgaa gccgagcgcc cgtcagcgca cgagctcgtc
     1801 ctccagggag cagcccagca cctccacgaa ctcggcctcg cggtcgcgca ggtagaagtg
     1861 cccgccctcg aacagccgga gcatgaacgc cccgctcgtg tgctgctccc acgcgcgcag
     1921 ctcgtcctct gacaccagcc ggtccgcgcg ccctccgaac gccgtgatcg gggtggcgag
     1981 cgcggggccg ggcacgtgct cgtaggtctc gcacgccgcc aggtccgccc gcgtcggcgg
     2041 caggaacagc cccatgaacg cgttcgactc catcaccttg ggcggggtcc cgccgaactc
     2101 gtcgagacgc gccgtcagct cgtcgtcggg caggacgtgc agtggatcgc cgcgcgcggg
     2161 caggtgcggc gcgcggaagc cggagacgaa cagccgcagc ggggcgggag ccccggcgcg
     2221 gtcgagctca cgcgcgagct cgtaggcgag cagcgcgccc atgctgtggc cgaacagcgc
     2281 gtagggccgg tcgagcaggc cgcgcacggc ggcgctcacg aacgtcgtca gcctgtcgat
     2341 gcggtcgatc gacggctcct ggacccgcgt gccgcggccg ggcagcgcca ccgcgcacag
     2401 ctcggcgacc gtcgacagcg gccccgccca cgggtggaac gactgggcca tccccccggc
     2461 gaacgggagg cagatgaggc gcatcggcgg gtcgggctgc ggctccggcc ggtggagcca
     2521 ggcgtcctcc gggcgggggc tcaactaccc gccgactacg gcggcggcgg ctgggtcggc
     2581 ctcggggacc ggggcgccgt tctcgccccg ctcctcgcga atcagctccg cgagccggac
     2641 ggcgagctgc gtgatcgacg ggccctcgat cagctcgacg atagggagct cgatctcgag
     2701 cgctccctcg acccggttct tcagctccag cgccatcatc gagtcgagcc cgatgtcgtt
     2761 cacgcggatc gcggggtcga gctcgtccgg gtccatctcc acgacacggc tgagctccag
     2821 ctggacgaac cgctcgagcg cgccgtcgag ctcgtcggag gccgccgcca ggatctccgc
     2881 cacggctgga tcggcctcgc ggccgtcgga cttcgacccc accaggtcgg cgatgagcgg
     2941 cgagcgcgcc atcgcgggga acagcgagcc gaacgcctcc cagtcgaagg gcagcacctc
     3001 ggagcgcgtc gcgtcctccg cgatgagctg ctccagcacg tcgaggccgc ggtccggagg
     3061 gatcgagccg gcctggtcca cgaccgagcg cgagtcctcg ttcgcgcgcg cggccatgcc
     3121 gatctcggcc cacggccccc agccgatcga cagcgcggtc tgggagtcct gcctgcgccg
     3181 gtcggcgagg gcgttcatga aggcgttgcc ggccgcgtag tgagcctggc cgggcgagcc
     3241 gagcacggcg gtggccgacg agaacagcac gaagaagtcg agcgggaggt ccgcggtcgc
     3301 gcggtgcagc gcccaggcgc cgatcgcctt cggcgccacg accgcgcggg tctgctcggc
     3361 ggtgaggttg accacgaacg cgtcctcgag gcggcccgcc gcgtgcacga tgccgcgcac
     3421 ctcgggcgct tcggagcgca ggcgcgcgag gacctgcgcg agatcctcct cgctcgtcgc
     3481 atcggccacc tccaggatca cgcgcgtgcc gcccgcctcg atctcggccg ccgcctcctc
     3541 gacctccttc ggggccgcgc ggcgcccgac gagcgccacc gcccccgcgc cgcgctcggc
     3601 cagccggcgc gcgacggcga gcccgagcgc accgtgcccg ccggtcacca ggtaggtccc
     3661 gtcctcgcgg accttcggct gcgaggcctc cccgtcgctc gccacgccct cgaaggagat
     3721 cgacagcttc ccgacgtgcc gcgcctgggc catcatccgg aacgcgcggc cgatctggtc
     3781 gacgcgctcg acgtggacgg gcggcggctt gaacgtgccg tcggcgaagc cctccatcac
     3841 ctcgtgcagc agctcgccgc catacgagtg gcgctcgcgg atcagccggt cgaggtccac
     3901 ggagtggaac gagatcgcct tgctgaacgg ccgcagcccc acgcgcgagt tctggaagat
     3961 gtcccgcttg ccgatctcga cgaagtggcc gaacggccgt atcagcgcga ggctccgcgg
     4021 gatcgcctcg ccgccgagcg agttgagcac gacgtcgacg ccctcgccgt cggtcagctc
     4081 gcgcacctcg tccgcgaacg cgagcgagcg cgagtccatc acgtgctcga cacccatcga
     4141 gcgcagcagg tcgcgcttct cgtcgctgcc ggcggtcgcg aacacctcgg cgccggcggc
     4201 ctgcgcgacc tggacggccg ccatccctac gccgcccgcc gccgcgtgga tcaggcagcg
     4261 ctcgccctcg cgcagccgcg cgacgtggcg caggccgtgc accgcggtca tgaacgcgat
     4321 cggcagcgcg gccgcctgcg cgtaggagag ctgctccggc ttcggcacca cgaggcccgc
     4381 cagcgtcgtg acgtgcgagg cgaacgcagt gggcgccacc gccaccacgt cgtcgcccac
     4441 ggcaacgtgc tcgacgccgt cgccgacggc cacgaccacg cccgagcact cgtcgccgag
     4501 gctgcgccgg tccgcggccg tgcccgggta gacgcccatg accttcatca cgtcgcggaa
     4561 gttgaggccg gtcgcccgca cctccagctc caccagcccc gggccgggcg ggtgccgctc
     4621 ggcgggcgcc agctccagcg cgtccagcac gcccgcggtc ctggtgcgca gcttgaaggc
     4681 cccgcgcgga gcggccagcg ccggcggtcc gccgttggag ctcgccgcgt cggtggagtc
     4741 gtggctcgcg tcgcggcgaa cgagccggcc cacgtgccgc ttgccgccgc gcagcgccac
     4801 caccggctct ggatccgacg ccagctcggc cgccagggag cgcgccgcgc ctctctggtc
     4861 ggccgccggg tcgatgtcca ccgccgcgac cttcagctcg gggtgctcca gcgccagcga
     4921 ccgcgtgaag ccgaagagca tcgcctgcgt cggcgagggt gcgtcgcccg agccgacgac
     4981 atgggcgccg cgcgttacga cctcgagccg cgtcctgcgc ttgcccgcga cgagcgcctg
     5041 gacgacgttc atgagcgagt cgcagccggc gccgagcgag ccctcgagcc agtcgtcctc
     5101 gcctggctcg ggcgcgtcga ggccccacag gtggatcacg ccgctgggcg actcgccctc
     5161 gccgaacgcc tggtcgatca cggtggtcac gccgtcgggc gcgaccacga agtcgtcgct
     5221 ttcgaggcgc tcgagcgcct cgccgcggaa ggcgagcacg cagcgaccgc ccgacgggcg
     5281 gatccgctcc gcgagtgcct ggccgatccc gccgcggtcg gccagcacga gccagccgcc
     5341 gtccgcgggg accgccgagg acgacgcgtc gctcgcctcg aaggcgagct cgtggagcca
     5401 gcgcgccggg tcgtcgctgc cgcccgcggc tccgcgatcg tcgatggcga gcaggtgcag
     5461 gccgcgcagc tcggcgacgg tcgcgccgcc ggcatcgagc acggccacat cggtgatcac
     5521 ctcgccgggg tcgggcgcgc ccgccatgtg cgcgtggacg acgacgctgc tgggaagcgg
     5581 cgcgtggaag gtgaccgagt cgatcgcgat cgggatcatc gggccctcgc ccgccagcgg
     5641 ctcgggctgg ccggccgcga tcgcgtgcag gcaggcgtcg agcagagcgg ggtgcgcgcg
     5701 gtagccgtcg ccggtcgcct cctcgggcag ctcgacctcg ccgatcacgt cgcgctcgcc
     5761 gcgccaggcg cgccgcaggc cgcggaacgc cggcccgtac tcgagcccgc gcgcccggag
     5821 gccgtcgtag atccgctcgg cggggatctc ctcgccgcag cgctcacgca gctcgtcgat
     5881 gtccgcgctg ccggcctcgg cgcccgcgcc gccggcggtc gcgcgggcgt ggagggtcca
     5941 cgacgtcgat ccgttcgacg agccgtcgcc gcgagcgctc tgcacggtga cctcgctctc
     6001 gccgccctcc agcggcacga cgctcacctg gacccgcgcc gcgtcctggc cgaagatgag
     6061 cggctggcgc agcacgacgt cgcgcagcac cttcggcgcc tcgctcgacc cgtggccggc
     6121 ggccgccagc gccatctcga ggtacgccgt ggccggaagc atcgcgcgac cctggaccct
     6181 gtggtcgtcg aggtacggat gccgctcggg tccgatccgc ccctcgagca cgacgttccc
     6241 cgtggcgagg cggcggccga gcagcgggtg gtcggcgacc gaggaggcgg acgcgccggc
     6301 gccggcgtcc tccgcgccct cgccctccgg gtcgatgtcg atccagtggc gcgtgcgctg
     6361 gaaggcgtag gtcgggagct gcacgggccg ccggtgccag cgcttgtgga atgccttcca
     6421 gtcgggctcg acgccgttga cgtacagggt cgccaggctg ccgagcgcgg tcgagaggtc
     6481 gtcctggttg cgccgcagcg acggcaccca ggcgcggccc gctccgccga cgcagtgctt
     6541 gccgagcgag atcagcgacg ggtgcgggcc gacctcgacg aacacctcca cgccgagccc
     6601 gagcatcgcc cgcacgctgt cggcgaagcg caccggtgag cgcgtgtggt cgcgccagta
     6661 gcgcgcgtcg ggcgcccggt cgagcgggcg gccggtcacg ttggagatca gcgggatccg
     6721 cggctcgcgg tagtcgaacc ggcccgccat gcgctcgagg tcgtcgatta tcggctccac
     6781 cagctcggag tggaacgcgt gcgagaccgc gagccggcgc gtggacacgc cctccgcctc
     6841 gaagcgctcg gtgaccttgt cgagctccgc cgccgcgccc gcgatcgtca cgttcgtcgg
     6901 gccgttgacg gcggcgagcg tgagccgcgc gccgacctcc tcgatcgtct tctcggcctg
     6961 ctcctcggag gcgagcaccg acgccatccc gccgcccgcc ggcaggtcgg acatcagccg
     7021 cgcgcgctcg gcgatcagca tcacgccgtc ctcgagatcc atcgcgccgc tgacgcacgc
     7081 cgccacgtac tcgccgacgc tgtgcccgag gaccgcgtcc ggctggatcc cccacgagcg
     7141 ccagagctcg gccagcgccc actccaccac gaacagggcg ggctgcgtgt agcgcgtgtc
     7201 gtcgagcaga cagtcgggtg ccttgccgtc gatcacgtcg agcacgtcgt ggtcgaggta
     7261 cgcgtccatc atctccgcgc agtcggtgag ggcgcggcgg aaggtcggct cggactcgcg
     7321 cagcgcgcgg cccatctcga ggtactgagc gccctggccg gtgaacagga agccgaccat
     7381 cggccgcacg ccgttgtgca cgtggccggc ggccgtggcg ccggcctccc cgctggcgag
     7441 cgcgtcgagc cgcttgcacg cctcggcgcc gttggccgcg acgatcgcgg cgcggtgctc
     7501 gaagtgcgcg cggccgagct gcgccgtgta gcaggcgtcg aggaacgact cctcgggcat
     7561 ctcggagcag gcgcgcgagt agcgaccggc cagctccccc agggcgtcgc tgctgcgcgc
     7621 cgagagcgtg agcacctggc gcgggcgctc cccgcggttg cgcgccggca gcggcgccgg
     7681 ggcctcctcg accaccacgt gcgcgttcgt gccgccgaac ccgaagctgt tgacgcccgc
     7741 cagccgcggt ccgccgttcg cctcccactc ctggagctcg cgcgcgacct cgagcggagt
     7801 gtcgtcgagc ttgatgcggc tgttgagctg ctcgaagtgc ggctgcgggt agatctcgcg
     7861 gttctccagg gcgagcaccg tcttgatcac gcccgccacg cccgcgaccg tctcggtgtg
     7921 gccgatgttg cccttcaccg agcccatcgg cagacggtgg gacgcgtcgc gccgcggccc
     7981 gatcgcgccg gcgatgccct cgacctcgat cggatcgccg agtggagtgc cggtgccgtg
     8041 cgcctccacg tagccgaggt cgtgcgcttc tatacccgcg cgcgcgaggg ccgagcggat
     8101 cacgcgctgc tgcgccttgg cgctcggcgc cgtcaggccg ttggtctggc cgtcctggtt
     8161 cacggcggtc ccgcggatca cgccgcggat gcggtcgccg tcgcggatcg cgtcggagag
     8221 gcgcttgagc acgatcacgc cgcagccctc gctgcgcacg tagccgttgg cggcctcgtc
     8281 gaaggtccgg cagcggccgt cgggcgagag catccgcgcg gccgagaacg cgatcgtggt
     8341 ctcgggggtg aggatgacgt tcgcgccagc ggcgagggcc agctcgcact cgtcgttctc
     8401 gagcgcctgg caggcgaggt ggatcgcgac cgacgccgac gagcaggcgg tgtcgacggc
     8461 gacgctcggc ccgtggaagt cgagcaggaa cgacagccgg ttggcggcga tgctgtgcga
     8521 gttgcccacc ccggagtgcg ggtcgatcat cttcagccgg cgcgcgagcc gcgcctggta
     8581 gtcgatgccg ccgatgccca cgaacacgcc cgcgtccttg ccgcgcaggc ggtggggatc
     8641 gagcgcgccg tcctcgagcg cctcccacgc gacctcgagc agcagccgct gatgcgggtc
     8701 gatcgagcgc gcctcgcgcg gcgtgatccc gaagaactgc gggtcgaact ggtcgatccc
     8761 gtcgaggaag ccgccccagc gcgtgcgcac cttgccgggg ctcttcagct cggggtcgaa
     8821 cagcgcgtcc gcgtcccagc ggtcgagcgg gacctcggag atgccgtcgc cgccggtgcg
     8881 cagcatctcg agcagcgcgt ccgggtcggg tgcgccgggg aagcggcagc ccatgccgac
     8941 gatcgcgacc ggctcctgcg tcccgtcgcc gttcgaccgc gcgcgctcac tcacgcgccg
     9001 gcccgtggcg ggtggatcgg cgggtgccgc cgcgtcctcg cccaggtgct ccgccagccg
     9061 gtcgatcgtc gggtgatcga agatcagcgt catcgagagg tcgcgcccga gccagcggcc
     9121 tagctcctcg gccagctcgg ccgcctggac cgagctcagc ccgacgtcga tcagcggcat
     9181 gcgcgtgtcg acggcgctcg cgtccacgcc cagcgcgccc gcgaggtggt cggtcagcca
     9241 gtgcgcgatc tcggccgagc tgcggggggc gcgctgctcg gcggcggcgg gcgccgggtc
     9301 cgcgccgctg tcgccgatct cgacgctcgc cgccgcggcc agcttggcct ccgcgtcgtg
     9361 gatgatcggc agctcgccgg cctggagcag ctcacacgtg gcgcgccgct gcttcttgcc
     9421 gctcgatgtc ttcggcccgc tcccggcctc gatcagcacc acccggtcca cctctacctg
     9481 gtgctcgcgg gacaccgcgc ggcgaatcgc ctccgcagcc tcggcggcct gctcgtcggc
     9541 gccgccgcgc agctcctgca cgatcgtcag cgcctgctcg tcgtcgccgt cgatcgcgaa
     9601 gacgacgccg cagcccgggc gcagcgcgct gtggctcgcc tccaccgtca gctcgacgtc
     9661 ctccgggtgc acgttgcgcc cgcgcacgac catgaggtcc ttcagccggc cggtccagac
     9721 gaactcgccg ctctcgaaga agccgcggtc gccggtgcgc agccagcgcg tatcgccctc
     9781 ctcgcccgcg atccgtgcct ggaacgtgcg ctcggtctcc tccggcttgc cccagtaccc
     9841 gcgggccacc gagggcccgg agacccacag ctcgcccacg cgcccctcgg gaacccgctc
     9901 gcaggtctcg ggatcgacca cctcgatccg cgtcccgcac cacggcgtgc ccagcgactc
     9961 gaggctccgg acgccgctgc cgggaccgtc gtggatcacg acctcgccgc gctcgagggc
    10021 gcgcgagtcc gcgtggatcg gccgcggccg cccggacgac accatcagcg tggcctccgc
    10081 caggccgtag gcgggcagga actgctcgcg ccgggctccg cagggaccga accgctcgca
    10141 gaacgcgtcg atcgtgtcgg gccgcacggt ctcggcggcg ttcatgaaga tgtgcatcga
    10201 gctgaggtcg agccccgcga cgtcctcgtc gcgcgcccgc cgcgtggcca gctcgtaggc
    10261 gaagttcggg gcacccgtga ccgtggcctt gaagcgcgac agcgcctcga gccagcgcat
    10321 cggccgctcg aggaagtgca gcggcgagag gtggacggcg tggaagccga tgccgatcgg
    10381 ccagagcacc ccgccgatca ggcccatgtc gtggtacggc ggaagccacg agacgccctg
    10441 gctgcggctc acctcgccct ggaaggtccc gtcgtggatc atcccgatgt tcttgacgag
    10501 gttgcggtgg ctcagcatca cgcccttcgg gaacgacgtc gagccggacg tgtactggag
    10561 gaaggcgagg gcagagtcgt cgacgtcggg ccgctgccag ctgtcggccg cgtcggggct
    10621 gacgtcgtcg gtgacgaacg tcggccgcga ctcgacgccc ggctgcgcgc gcaccatctc
    10681 ctcgaagtcg cgcacggtga ggatcacagc cgcgtcgcag tcgtcggcga agcgggcgag
    10741 agggggcgcg agtcggttga ggccgggcgg gtagagcggc acggcgcaca cgccggcgta
    10801 caggcagccg aagaacgcgc ggatgtagtc gagcccgggc gcgtacacga gcagcgcgcg
    10861 atcgccgggc tgcgtgcgct cggccagccc gacggcgagc atgcgcgcgg cctcgtcgag
    10921 ctcgccgtag gtgacgtgtt cctcgtccag ctctccgtcg cgcaggaacg tgaacagacg
    10981 ccggtcgggc tcgtccgcag cgcgctgccg gagcagctcg atcaaggttg gatccgtcac
    11041 cagcgccccc gcgcgggctc agctaccacc gttaaatacc cctcctcgtt cccccgagcg
    11101 cctgagcgat ccggcgggcg ggaacgttac cagtagttaa ccgatccact gcgccacatc
    11161 gatgtgggat cgtttcacga gtgcggggca aacggggcca ttccggctac tccggagacg
    11221 gtccctccgg cgcgggttcg aagcggatct ccgagccgac ctcggccccc aggtcgtgcg
    11281 ccgtcagcag gcggtttgcc gcgacgagga cgttcaggtg gttcgggaag tgacagtcgt
    11341 cgaagccgag gacgcggccg aactcgactc cccccgccag cgccacgtca ccgcgctcga
    11401 ccactccgcc gcggtcgagc tcggcgaagc cgacgaagcc gacgcggtcg atccgctcac
    11461 ccgcatccgc gtcgcgctgg tcggttgtca cgatctcgtg gatctcgccg cgcagcacgc
    11521 agcgggtctg gtagtccgca agcgtcatgc cgcgctcggt gcgggtgtgc tggaggagct
    11581 tcacgagctg gccgctcacc ggggacttcc actccttcac gcctccccct cgaccgccgc
    11641 cgccgcgggc tccgccagcg cgtaggagcg ctgcaccagg cgcacgaccg acacgcccgg
    11701 ctcgtcgccg cgctcgccgc gctcgacgcg cccggcgaag tcgcgcagga cgccgacgta
    11761 ctcgtgctcg agcgacgact tgaacgcctc gtagcccgcg agcatgtcgg cctcgagcgt
    11821 ctcgccgtca gcggtctcga tcgtcacgtc cttcagctcg ccgtgctcgt aggcccagtc
    11881 gagctccacg gtcgcgggca cgccgccggc cgtgcgcagc tcgacccacg cctgcaggtc
    11941 cacgccgcgc tcgtcgcgca cgatctccgc ggacaccggc tcgagcggcc cgagcacgtg
    12001 ctggagcgtg tcgatcgcgt tcggcccgtt gtcggccagg cagccgccgc cgcagcgcac
    12061 cgggtcgagg taccaggtgt cggagccggc gtgctcctcg atcttctcgt ggtagcgcgc
    12121 ccgggcgctc gccacggtgc ggtcgccgag ccgctcgacc aggtcggcca ggttggcgtt
    12181 gtagcggcgg tggaaggcgg tgaagagggc ggcgccgtgc tcgcgggagg ccgcgtcgag
    12241 cgcgtccgcg tcggcctcgg tcagtgccag cggcttctcg cagcacacgt ggcgcccggc
    12301 gcgcagcgcc gcgccgcaga cctcggcgtg gaggtcgttc gggaggttca cgagcaccgc
    12361 ctcgacctcc tcgcgctcga gcgtcgtgcg ccagtcacga tcgcacgcga caccctggcg
    12421 gcggtagggc tcgaggcgct cgtcgttgcg gtcgcacacg gcgaccagct ccacctcgga
    12481 cagctcctcg atcgccagcg cgtagtagcg cgagatcacg ccaagcccga gcatcgcgac
    12541 gcgcagcgcc atctacgcgc tcgtcccggc gccctggggc agccacgcgg gcgggtcgat
    12601 gccgagctcg gtggcgagct ggtcgagctt gctcaccacg tgcggcgcca ccgggatccc
    12661 gttggcggcg cgctcggcgg cgctgtcgct ctccacgtgg cccggataga gcacgcgccc
    12721 gccctcgcga gcgggcgggg agccgagcag ggcgccgagc atggcgtcca tcgtcgcctc
    12781 gaagtcggcc acgggccgga aggcggagac gtcgagcgcg aggatgaagt ggcccacgtc
    12841 tctgtcgtcg gctcccggct cgcccagggg cccgacgccc gcgccgggca gcgcgccgca
    12901 gaggatgtcg accagcaggc cgaggccgaa gcccttgtag gcgccggtct cgggccggcc
    12961 gccgagccac tggatgtggc cgcgcccctc gtcgagggcg gccgggtcgg tgaccgggcg
    13021 gccgtcgtcg tcgaccagcc agccctccgg gatggtctcg ccgctcgcgt gcgcggtgcg
    13081 cacgcggccc gtgggcacga cggtggtgct catgtcgagc acgtaggagg gcaggtcgcc
    13141 ggccggggcg ccggcgctca gcgggttcgt gccgagcatc gcctcgccgc cgcccggcgg
    13201 gcgcgcgatg cgctgcgtgc cgcagttcga catggccatc gcgaccatgc cctcctcggc
    13261 ggcgcgcgcg ctgtagtagc cggcggggcc gaggtggctg ctgcggcgca cggccaccgc
    13321 gcccaccccg tgctcgcggg cccgcgtcat ggcctcgtcg agcgcgacca cgcccacgta
    13381 caggccgagc ccgtcgtgcc cgtcgagcag ggcggcgccg ccgcgctcgg aaaccacctc
    13441 gggacgcgcg cgcgggtcga cgcggccgtc gcgcagccgc ggcacgtaca ggcccgcgag
    13501 gttggccacg ccgtgggtgt ccatcccgac cgcgtccgcg taacacagcg agcgcgccgc
    13561 ctcggcggcg gggccgggct ccatcccgca gatgccgaag accgccgcgg tgaacgcccg
    13621 cagggcatcg ggctcgatgc gcggccggtc gctcacgaag ccagccccgc ggcgcggtcg
    13681 tcgaagtcga gcacgagccg cgccgccgcg cgggtgtagc tctcgaggtc ctcgcggcgc
    13741 acgaactcac cggccgcgtg ggcccggttc ttgcgcaggg tgccgggtcc gaagacgatc
    13801 gagtagccgt ccgccagcga gccccagatc gcgtcgcagg tgaagcgctc gtgcgagcgc
    13861 gactcgggca ggcgctcgat gccgaccgcg gcgagccggc cctcgaactc ggcgtcgcgc
    13921 ccctccagcg tcggcagccc gcgcttgacc cactcgacgt gacagatgcg ctcggccgcc
    13981 tgcgctgtgc gagccgccac cccggccccg gcgaagcgct cggcgaagga ggcgcacgca
    14041 ccgcgcaccg cctcctcgag ctcgcgctcc atggtctccg cggcctccgc cgaggggtag
    14101 gggatgttca ccatcagcgt cccctccccg tacacgcggt cgtgcatcgt gccggtggtg
    14161 agcccgccga cgcagagctt gcagcccgca cgggtggcgg ggccgtcgag gcgcgccgcc
    14221 agctcgaccg ccacgtgccc gagcaggagc gtggcgttct cgcccgcgtc gggctcgtcg
    14281 tcggtggacc cgctgccgcg gacggtcacg cgcgcggtca tcgacgcggt gcaggagtcg
    14341 aagtagaccc cgccggtggg ctcggcgaac acgttgagcc gcccggtcag gccgcgctcg
    14401 acgagcaccc gcgtgccgta gctgcccatc gcgccggact cctccccgga tacgagctgg
    14461 agccgcaccg cgatgcgctc ggtcaggtcc gggcgctcgc gcagcgcgtc gcgcacggcc
    14521 gccagcagcg ccaccgccgg gcccttcatg tccaccgcgc cgcgcccgct gatcacgccg
    14581 tcgcgctcgc cgaccggcac gtcgccgcca accgtgtcga ggtgcgcgtt cagcatcagc
    14641 gtccgctcgt ggtccgtctc cggaccgagc cgcagcacca tgttcggctg gcacgcgagg
    14701 aactcgtcgc ggcccatcgc gtccgcgacc tcgcgcaccg gcgtgggcac gccgggcgcg
    14761 tcgagcgcgg cggcgggcgg cggctcgtgc agctccacct cgaacccgag ctcggcggcg
    14821 ctctccgcga tcagcaactg cgcgtcgccg atagccgacg gcatgcgggt ctccatcggc
    14881 gtgacagtcg gagtgcgcag cagctccagc aggagccccg cgtgcgcgtc gtcgaacgcc
    14941 ggcgcggtgc gcgactcgct cacgacatcg gcgccacccg acgtggctac ggctgggagt
    15001 cctccgcctg gcacacgacc gggctctcga ggcactcgtt ggtgtggtcg atcgactgct
    15061 tggcggcgtc gagcgactgc ttgagctgct gaagcgtcga ggtgatcccg acgatgttct
    15121 ggcccaggga gctgacggag ccgttggtcg tgcgcagcgt gttcgacacg gcgccgagct
    15181 ggtcgctggt cccgaggagc gtgttgttgg tggccccgag gatgcgggtg gtggcgccga
    15241 gcgagcggtt ggtggctccg atccgggccc cgagcgtcgg cacctggcgc gtgaggctcg
    15301 tgagcgtgac ctggagctgc ccgatgtcgc gcgcgagcga gttcagcccg ctcgtctgct
    15361 ccagcagccg cagcagcagc acgttggtgt tctggagccc gccggtgttc tggacgagct
    15421 gggcgatgaa cggcgtgagg gcaccgaggt tcgcggccct gaactcctcg agcagcgcct
    15481 tggtcgtcgc cgtcgtctgg tcgagctcct tcaggagggc cttgcccccg ccgggcgcgg
    15541 aggccggagg gagcgccggc ggggcctggg tcgtgggggc attgtcccca ccgccgtcct
    15601 tcgtgtggtc ggccacctgc cagagcagga acgtcgagac cgtgaggagc aggagcgcga
    15661 acagcgtcag ggcgaacatc gcccgcagga gcgcggaggg ctgctccccg cccccgccgg
    15721 acggcgccgg ccgcggcagc ggccggcgcg gaggcggatc gaccccgaag tcgtcagtgt
    15781 ccggcatcgc ccgccttgcc tcagtcgccg ctgaacgact tgagcttcca gtcgtacttc
    15841 agcttcacgg acgacgcgcc caggttgcgg gtctggtcga ccttctgccg gaagagcgtg
    15901 ccgtcgtcga gcgagacgta cgtctcctgc gtgctctctc cgctcagctc gcccttgaaa
    15961 gcgaagtgca gcttcacgcc cggggacttg gtgctcttgc cctcgaagct gaacgactgc
    16021 cgcgggagca gggtgccctc gtaggtgccc tgcgtcgggc cgccgaagct cccggtccac
    16081 tgctccgcgc cactgccgtc gacccccctg atcggcgggt tcggccggaa ctgagccgtc
    16141 tgcccggcga cgaaggagtc gaggctcgtg acgtagacgt cgccgccgcg gatcacgaac
    16201 gtggtggtgt cggcctcctt cggcgagtag gcggtctgcg aacggaagca gacgaggttg
    16261 cccaccttga tggtcggcgt gacccggagc tccgcggtct tcggcagcgc ggtgccggag
    16321 cccgagccgg actcgctgcg ggtgcccttc tggtcgtagg tgtaggtgcc gggcttcggc
    16381 gcgaggttgt cggcccgttc gcccgtcttc agcgcatccc cggcgcagtc ctcgggcttc
    16441 ggcgtctcgg ggggctgcag gctcgccgag ccgccgccga gctcgtcgcc ttcgtccttc
    16501 ttgtcgtccc ctccacagcc ggccagggcc attacccccg cagccgccag gatcacgctc
    16561 gtcgtgcgca gactcgccat ccaccctccc caggtcctgt cgctaaagaa cgagcgatga
    16621 taaacgtcgg gcggcgcctt cacgtcgtta ccggacgcgc ctgcaccagg gtccactgct
    16681 cgccgtcgaa ggccgcctcc acgtccaccg ggcattcgta ggaacgctcg accgctaaca
    16741 cgatctcgac gaggtcgttg aggtcggcca gcgagatcgg ccagggcccg ctggccccct
    16801 ccacctcgag cagtgcgcgc gtgccacggt cgacccggaa cgtgaccgca tcctcgccgc
    16861 cggccatgat cccctccccg aaggactcgc acgtggcgac cagcagctcg cgggtggagc
    16921 ccgtgaccgg gtccttggtg aacgcgaccg ccgagccctc cgcctccacg agggcctgca
    16981 cgaccaccgc catctcggtc tcgaccccga gcgagcggcg ctcgcggtac gccatcgcac
    17041 ccgccgacca gagcgacgcc cagcaggtgc gcacggcctc ctcgaccgcc tcggccgtgc
    17101 actccacgcc gagcacgctc tcgtgggcgc ccgcgaacga ggcgctcacg ccgtcctcgt
    17161 cggtcgcgga cgagcgcacg gccaggcgcg cgccgtccgg cagcgcggag cgcagtgcgg
    17221 cgacgcgctc gtcgagcgcg gtgcgcaggg gagcgggcag tgctgaggcg cgagccagct
    17281 cctgcagctc ggcgcgcgcc tcctcgtcgg gcagccgcgc gcggagcccg tcgtagcgct
    17341 cggtcaggcc ggcgccgtcg cggtaggcgc ggtgcgccgc cgccgtcagg cagaagccgg
    17401 gcggcgtggc gaacccgagc cgcgccagcc ggtccacgct cgcggccttg ccgcccacca
    17461 gcgtgacggc gggcggctcg ccctccccga gcccgtgcac cagggcggcg cccgtcgcgg
    17521 aggtgcgttc cccggtggtg ccggtcatcc gacggccact ccctgggacg cgaggctcat
    17581 ggccaggccc tcgagcaggt cgccctccgc gagctggagc cggtcgaggc cgaaggcgtc
    17641 catcgtctcc atcacgatcg ccgtgcccgc gacgagcgtc ggcgcgcgcg cctcgtggat
    17701 gccgggggcc tcgcgcagct cggccatccg ggcgccgatc aggcgatcga gcgcctgctc
    17761 gatcagcgtc cggctgagaa tcccggactc cgccgcctcg gccccctcgt cgccgagcgc
    17821 cgtcagcgcc ggccgccagg tccagccctc ggccgacatc agaccgtgcg cgaggccggt
    17881 gcgcatctcg gcgggcacgg cgtcctcgag cacgcgctgg atgttcgcgc gcagcgacgc
    17941 gacctcctcg tagcggggcg ggtcgccgtg caggaagcgg gcggccttgc gcaccgcgcc
    18001 cgcctgggtc gacgccttga agagcggcgt cccgcgctcg ccgatcatca ggtcggtcga
    18061 gcctccgccc acgtccacca cgagcacgcg gccgtcgaag tcccgcccga gcgtggcgcc
    18121 gtggtaggtc agctccgcct cggtgtgccc gtcgatcacg cgcgcgttga tgcggaagcg
    18181 ccgccgcacc tcggccgtga actcggcccc gttctgggcc tcccggaccg cgctggtgag
    18241 cacggcgccg ctcgtgacgc acgagtgctg ctcgatctcc gcggcgtgct gcgccagcgc
    18301 cttgagagcg cgcgcctgcg cagcgacggt gagctgcccg cattcgtaga gcccggcact
    18361 gagctgcgtc gggatcgacg tgcggacgac cggcttgatc gcgttgggcc cgtcgacgtc
    18421 ggcgatcagc agacgggtcg agtcgctgcc cacgtcgacc gcggcgacgc gcgtgctcat
    18481 cccgccgccg ccccgccgcg cgcgggctcg gccactcgct ccatcagccg ctcgagcgcg
    18541 cggccgcact ccacgaacga cgcggtggcg ccgtcgcccg cgagccgctc gccgcggtgc
    18601 ggcgcgacgt gcatgtgcgg ctcgatggtg aacccaccgc ggtagccgga cgacaggagc
    18661 agggcaaggc actcctccac ctgcccgtcg ccctcgccgg gcgggacgta ctcgacgtcg
    18721 tccccgcggc gaaccgcgtc cttcacgtgc acgtgctcgg tccagtcgag cgtgggccgc
    18781 aggaagtcga gcgagtcgta gccgtagctc agcccgttgc cggtgtcgaa cagcagccgc
    18841 agcgcggggc tgtcgacgcc ggccatcagc tcgaggctct cctccgggcc gcggccgccg
    18901 tagcccgcgc agttctcgtg gagcagcgtc aggccgagct gctcggcgcg gcgcgccagc
    18961 tcgccgaggc ggcggcggga ctcggcgcgc cagccggcgt cgtccacgtc ctcctggagg
    19021 aacgacatca cgcgcaggcg cgtgcagccg agggcggcgg cccgcggcgc gagcgcctcg
    19081 agctcgcgct cgtcgacctc gagcggcgtg cccgtggtcc gcccccaggc gccgatgccc
    19141 gacgagacgc cgacgacctc gaggccctcg gagcgcagct cctccgccac ggcggacacc
    19201 gcgtcgagcg gcatgtcggc gatctgcgcg ccgtcgatcg tccgcagctc gatgtgcgac
    19261 cagccgagcc ggcggtgcgc ggcgatctgg cccgcgaggt cgggcgcggc ctcgtcgccg
    19321 atgccggcga gcgcgacggc ggacggctca gcggccatgg tgctccagca cggcggcgat
    19381 cgtggcctcc acgtcggcgt cgggcatgtc ggggtagagc gggagcgcga gcgactcgcg
    19441 gcacgcgcgc tcggcgttcg ggaagtcccc ctcgccgtgc ccgagccacg cgaacgcggg
    19501 ctggaggtgc agcggcagcg ggtagtagac cttggtctcg atgccgcgct cggccaggtg
    19561 acgctcgagc ccgtcgcgat cccggcaggc gacggtgtag gtgtagaaga cctgctcgtg
    19621 gtcggccggc ggcggcacgc ggaggtccgg ggcgaggtcg gcgagggcct cgtcgtagcg
    19681 ggccgcgatc tccgcgcggc gcgcgatcga ctcgtcgtag cgggccagcc gccgcgacag
    19741 gagggctccg gtcacctcgt ccatccgcgc gttgaagccc acgatgtggt ggaggaagcg
    19801 ggtgatgccg tcctgcccgt gattgcgcag catccggcag cggcggatca gctcctcgtc
    19861 gtcgccgacg atcgcggcgc cgtcgctcgc gcctccgatc ggcttgaccg gctggaacga
    19921 gtagagcccg atgtcgccga agaggccggc catccgcccg tcgcggcgca tgccgatcgc
    19981 caccgcgctg ttctcgagca cccaagcgtc gtgctcgcgc gccagggcca cgatcgccgg
    20041 catgtccgcc atctgcgtga acaggtgcac gggcaccacc ccggccgtgc tctctccgat
    20101 cgcgtccgcg agccggtccg gatcgaggtg ccaggtctcg gggtccacgt ccacgagcac
    20161 cggcttgccg ccgcggcgcg ccaccgcgct tgccgacgag tggaagccga aggccggcac
    20221 gatcacctcc accccggggc cgaccccgag cgccgtcagc gccacctcga gcgcgtgagt
    20281 gccgctggcg accgcgaccg ccccgccggc gccgctgtag cggcacagct cctcctcgaa
    20341 ggccgcgacc cggcgcttga ggatgaactc gtcggagcgc gcgacgtcga gcagcgcctc
    20401 ctcgatgaag gcccggtccg ggtgcgtgcg ccggtccatc ccgtagtagg gcacgctcgg
    20461 cgagtcgagg atcatcggac gccctccgcg tgctcgacgg cgcggcccgg cccgccgccg
    20521 gactcgtaaa agccggcgat cgcggcgcac acctcgtcga cctgcgcgtc gtccatgtcc
    20581 gggtagagcg ggagccccac ggcccggccc gccgccttct cggccacggg gaagtcgccg
    20641 gggccgtggc ccatgtccgc gaagcagggc tggaggtgga gggggcgcgg gtagtagacc
    20701 tcggtctcga tgcccgccgc cgtcaggtgc tcgaccaggg cgtcgcggtc gtcggcctcg
    20761 atcaggtaga cgtagtagac cgccgtggcc cgctcgtcca cgatcgccgg cgtctgcacc
    20821 acgccgctca gccgctcgtc gtagcgggcc gcgatctcgg cgcggcgcgc gatgtccgcg
    20881 tcgagcgtct cgagcctggt gagcagcacc gcggcctgca cgtcgtccat gcggctgttc
    20941 atgcccatca cgtcccagac gtacgggcgc tcgccgggca cctggccgtg gttgcgcagc
    21001 cggcgggcga gctcggccac gtcgtcgtcg tcggtgagga ccatcccggc gtcgcccagc
    21061 gcgcccagcg tcttggtcgg gaagaacgac aggacgcccg cgcgcccgaa gcgcccgccc
    21121 ggcgtgccgt cgaagtccat cccgatcgcc tcggcgctgt cctcgaccac ggtgagcccg
    21181 tgacggtccg cgacgtcgga gatcgcgcgc atgtcggcca tctgcgtgaa caggtgcacc
    21241 ggcatgacgg ccttcgtgtt cggcgtgagg cgcttctcga ggtcggcggg gtcgatcgcg
    21301 tagctctgcg gcttcacgtc gacgaacacc ggcctggcgc ccacgtgcac cacgctcgag
    21361 gccgaggcga agaacgtgta gacgggcacc gcgacctcgt cccccggccc gatgccgcag
    21421 gcctcgagcg ccacgatcag ggcgtcggtg ccgctggcca cgccgatcgc gtggcgcgcg
    21481 ccggtgtact cctccatcgc gcgctcgaac cggcccacca ccgggccgtt gacgaacgtc
    21541 ccggagtcga acgcctcgtc cagcagctcg cgcacgcgcg accagcggcg ctcgagcgag
    21601 cggcccccgc tgaagaacgg gaccgccatc agcccgggac ctcggccagc ggctcgtcga
    21661 cgatccggcc gggctcctcg atcccggccg cgcgcttcgc cgccgcgatc gtgtcggtca
    21721 cgagcacgtt gaagcgcagg tcgctgaccg gacgcgggcc gaggtccgcg tagtgcgtgt
    21781 accagtcgac catcatcttc gcgagctggt cgtccaggag cagctcgcgc tcgcgcggcg
    21841 gtccgccgtc gggcatcacc tggagctggg cgtaggtgtc gtcgccgacc gggtagtggc
    21901 cgagcacggt gccgagctcg aagccgagct cgatcgaccg cgcgcggacc ggcgccgtca
    21961 ggtcgcagtc gataacagtg ctcacgcccc cgcggtggct cagctcgacg ctggcgcggc
    22021 cgaggtgcgg ataggccacg ccgtcgagga ccatgtcgct cgacccggcg ctgacgacct
    22081 cggcctgcgt gccgccgaga tgaagcgcga cgcccatcga gtgcgggacc tcgatctcga
    22141 acgccgtcgg atggtcgacc ttgtcgcgcg tgcgcgtgaa gcgcggcttg agctgctcga
    22201 tcttgatcgt cttcagcctg ccgaaggtgg ccaggtcgac cgcctcgcga agcgagaggg
    22261 tgagcgtgct cgacagccac gggctcacca ccgccacgtc gagctcctcc tgctcgacga
    22321 tctcgaggat ctcgtggagg tcctcgaccg accccgcgac cggcttctcg cacacgatcc
    22381 ggcggtagcc gcgggcgctg agctggcgca gcacggccac gcgcgtggcc ggcggcgtgc
    22441 acacgtgcgc gacggtgtcg gccggttcga tcccctcgac ctggtcgagc gagcggacga
    22501 gcaccacgct cccgtcgccg cgcgcgcggg ccttcgcgta catcgccggg tccaccgcga
    22561 cgacgggcga ggtggcgaac acgggctcgc ccttgcacag cctgtgcgcg tgcaggaggc
    22621 tgcgcaggtg cagtgcccgc cccgagttcc cgaagccgag gatcagcgtg ctcagcacga
    22681 cgccaccgtc acgctgactc cgtggccttc tgcatgtgct cctcgagcac gtccagcagg
    22741 tcgtcgaccg tgcgcacctt catcagccgc tcgtcctgca tcaggtcgat cccgtaggtg
    22801 tcctccagcg acgccagcag ctcctgcgcc agcagcgagt cgatccccag caggtcgagc
    22861 cgatcctcgg gccgcacggc ccgcgacgtc cccttgagct cctggtagta gcgctcgaac
    22921 tccacgcgaa cgcgttcgcg atcagtctcc aatgaccgtc acctcccccg tagtcccgtc
    22981 aaggcttacc tcctgcccgg gttgaagctc gcgcgccagc gcgtggccga ccaccgcggg
    23041 caccccgtac tcgcgggcaa ggatcgagac gtgcgagagc gcgctgccgc gcgcggtgat
    23101 cacgcccgac atctgcggca ggagcggctg gatgtccgcg tccgccgagt cgcacaccag
    23161 gatccggcgc gcgccgttga gctcgccgtc gagctgcggg ttggacggcg agacgaccag
    23221 gccggttgcc agcccgggcg agacgccgcg gcccttgccg aagcgcttct cgcgcccggg
    23281 cgcctcgatc ggcgcgccgc cccggcgccc cggagccacc agctcgggcg cctcctgggc
    23341 gagcccgcgc tcgcactcgg cgcgccggcg ggccgcccgc tcgctcgcct ccgccaccga
    23401 cagcgagccg tcgagctccc cgaccgtcag gtacggccag tcctcgtggg ccacgcccgc
    23461 gctctcggcg gccagcggca gcagccggcg gaacacgtgc atgcagcgca tggcctgcga
    23521 cttgctgttc tcgcgcgagc tcatcgtgtc gctcaccagg cggtcgagct gcggcaggag
    23581 cgcgcggcgc gagcgcggca ggatccccca cgccacctcg cgcaggtcgt cgcgctcggc
    23641 gggcggctcg gcgagctggc cgccgccgtg acgggtcgcg tacgtggccc acggctcgtg
    23701 gtggtcggcc acctcgtaga acgcgctgtc gaggaactcg ccggcggcga gcggcgtcgg
    23761 gtcctgcgac gcgtcgtgga gcatcgacca gaccagctcc tgcgggcggt tgagccacgg
    23821 ctcgagctcg ggccagtagc cgagcgcgcg ccggccgatg ccgcgctgcg ccgcgcgcag
    23881 cgggaccagc ccgagcgtgg tcgtgtagtg gatcttccag atctcgggca gcagcgcggt
    23941 ggcgcgctcc cagcaggcgc cgagcgcgaa ctcgctgccg gagtcgaccg cccggcgcgc
    24001 ctcctcctcg agcaggtgga cccggccatc cgactcggtg agcgcaccgc gcagcgagac
    24061 gccctcgcgg gcgatccgcc agacgccgcc tgcccggccg agcgtcccgg cgcccacgcc
    24121 cttggggcgc tccggctccg gggcgtcctc gaagtagctg ccgatcacgt cctcgacgct
    24181 cgtgccgggc acctcgcagc ccatgtggca gaacgcggag aggttgtggt acgggcggca
    24241 gcggaagtag ccgacgaaga cgtacagcga gcccgtgtac caggtcgccc gcggccagag
    24301 catctgcgcg agcgcgcggc agccgcgctc caccgggtcg ccgatcagcg accacgcggc
    24361 gatcgagagg cgctcggggg cgacctcgcc gaagttcccg gccgagtaga cggggaagcg
    24421 cgggtgcacc tcggtgtcga tcgacaggcg cccctcggtc atgccggcac cagctcgctc
    24481 tggcgggcgt ggagcgccgc gctcgtcgcc tccaggtagc cgggctcgca acgcggcagc
    24541 tcgcccggga tcgcctcgag cacgtcgggc gccaggtcga cccacggctc cgagtacgag
    24601 agcagcacgt cgggtgcgcc gaagcgccgc gcgagcagct ggctggcgcc gccccaggcg
    24661 cgcgcctcca cgatccgcgg cagcacgttc caggccgcgc ataggccgac gaacacctga
    24721 cccagcgtgg gcgagtccgg gtcgaaccag gtccacgcgt cgtggtcccc gggctcgaag
    24781 agagcccggc gtgtgacctc ggcggccgcc gcggcgtcgg tcgggtagca cgggaacgcg
    24841 ccgcggtcgg tgaggtggac cgggtagccg cgcaccagcg gcggcagcgc ggcgaggatc
    24901 ccggcgtgtg ccgacggcgc gcccgccgcg cccacgccca gcacggggcc gagccgcagc
    24961 acccgccacg gcagcgactc ctcgagcctg accctgcgct cggcggtgaa cttcgcgtag
    25021 tcgtaccagc tccggaacgt ctggccgacg tccaggtcgg cgttgcccac gacgccctcg
    25081 gcgcggccga gcgcgagcac ggacgagacg tgcacgaggc gctcgaggtt cggcatccgg
    25141 gcggcgaggt cgagcacgtt gcgcgtgccc gcctcgtgca gctcgacgat ccgggggccc
    25201 gagtcccagt ccaccgtgcc gaacgacgag accacgtggg tcacgctctc gagaaggtcg
    25261 cgcagggcgt cgccgcgcag ccccatgtcg ggcgcgcggg cgtcaccgga cacgccctcg
    25321 ccggccacgg cggtgccgga gcgcgagagc gagacgacgt cgacgccgac cgcggcgagc
    25381 cgcgccacca cctcggcgcc gacgaagccg gtgccaccga ccacgaggac cctcatcccg
    25441 cgaacctcgc gagcgccagg tcgccctgga gcggcggcgg ctcggccgcc tcggactcga
    25501 acgccaaccg tgcgagctgc tcggccgacg gcttgggaag cggccagtcc accagcgacg
    25561 ccatgcgcgc gcgctcgaac acccggtcga gcgcgagata gcgcagcccg accagcaggt
    25621 tctgctccgc ggcggggaac gagcgcagcc ggtcgaggtt gttcgagctc cacacgagcg
    25681 cgcgggcggg cacgggaacg cgccgccgcg acggcacgcg gccctcggcc acgtagtcga
    25741 tcgcggccag cagggcgtcg gtggtgggcg ccgcctcgcc cgcgcagacg tgggcgatcg
    25801 ccgattcgcc cggcggctgc ccgcgcaagc gcccgatcat cccgagcagc agccgggcgg
    25861 cgtcgtcgcg cggcagcatg tccacccggc ccttgcggac gtagggcatg aggccgccgg
    25921 gcatcgactc ccagcggtcg gcgagcatgt agagcgagtt gcgcttggcg gtcgcgccgg
    25981 tgaccgagtt gccgaccagc ccgccgatcc gcgcgatcgc cgtcggcacg ctccggcccg
    26041 cgtcgatcag gcgccgctcg gccagccact tggactgctc gtacgcggtg cgctcgccac
    26101 tcgcggggaa cggcagctcg gccacctggc cgcgcacggc gccggcggcg tggatcgagc
    26161 tggcgtagca gaacagcggg gcgtcgccgt gctcgccgat ggccccggcg agctgctggc
    26221 cgttgagtgc gccgagcacg ttcaccgcgt gcaggcgccg cgtgggtgcc gcccagttcg
    26281 tctcgcccgc gatgtcgagc accgcgtcga ccgtgcccgc cagccgccgc acgcgcgggg
    26341 cggagaggcc ccagtacggc gcggtcacgt cgccctcgat cgcgccctcg gcgagctccg
    26401 gcgcatcccg gaacatgcgg ccgagccgcg ccttcgcgtc ccggcggccg acgcggcgcg
    26461 tgagcggcac cagctcgacg tcgtggtcgg cggccacctc gaccagcccg tgaccgagct
    26521 gcccggtgat ccccgccacg aggatcctca cgccatctcc tggacgtggg cgtcgtacag
    26581 ctcgtcgttc accggcagcc ccgccgcgac cagctcgagc atgatctcgt cggtcccgcc
    26641 gccgagccgc gcgaagcggc cgtcgcggaa gaagcgcgcg agcggcgtgg cgtcctcgag
    26701 gtagccgagc ccgccgaaca tgttcaggca ctcgctcgag gcgcgctcgg cgatgcgcgc
    26761 gagcgtgacc ttgagggcgg cgacctcgcg cgcccactcg ggccgcagcc ggtcgagcga
    26821 cgccgccagc gcatacaggg cgaggcgccc ggtggtggcc tgcgccgcga tctcggcgag
    26881 ccgcagccgc agcgtctggt gctcgatcag cggcgtcccg aactggcggc gcctgtgcag
    26941 ccgcgtggtg gcgagcgaga ggccgaggtc ggcgccgccg atgagctgcg cgaccgaggc
    27001 gagccgctcg tgggtgagcg cccacgtcgt gatcaggaac ccgcgcccct cgcggccgag
    27061 cacggcctcg tcgggcacct cggcgtcgat ctcgatgcga accgtctcga gcccgcgcat
    27121 cccgagcggc ttgagccggc gcaccacgcg gaagccgtcg ctcgggacgc agaccacgct
    27181 gagcggcggc ccgatcgccg agcccgcgct gcgctcggcc gccgccgagc cgtcgccgct
    27241 gcggcagagg acgagcgcga agtcggccac ggcgccgagc gaggcgaacg ccttggtccc
    27301 cttcacgtgc cagccggcgt cggtgcgcgt cgcggtggtc cgcatgccca gcaggtcgga
    27361 gccctcaccg tcctcggttg ccgcgaagca gccgatctcg gtgcagtcga gcgccgcctc
    27421 ggtgcgctgg cgcagcaggt cgctcttgcc gaagcgggtg agcgcggcca ggaccacgtc
    27481 gaggtgcacg ctcacgccga cgcccacccc gccgagcccc gcgcggccca gttcctcgga
    27541 gatcacgacc cccttgccgg cgtcgccgtg cgggccgccc gtccatcgct cgcgcgcgag
    27601 accctcgccg ccgatggcct cgatcgccgc gcgcgggaag cgcccgctct cctccgcgtc
    27661 gggcacgagc ggcgcgaccg agcgcacgcg ttcggccacc agctcgcggt acgcgcgaac
    27721 ctcctcgctg tcgatgccgc cgctcagcag cagcggccgc tcggtggcgg tcgcgctcac
    27781 gcgccgacct cgctcggcct ggccgccggc gccccctccg gcgtgatcga gcccaccacc
    27841 tccgcgtcga tcccctcctc cgcgtatatg cgccacatga cccggcgcct caccttgccg
    27901 ctcgaggtgc gcgcgatcgt gcccgccctg ccgcgcagca actcgacgcg cacggctccg
    27961 cccgtctcgt cggcgaggat ctgaagcgcc gtgtccgccc actcggcggt cggcccctcg
    28021 accacggcca ccacggtgtc gccgtggtgg ccgctgccgg tgaacaccgc gcagcggccg
    28081 atgccgatcc cctcgacgag cgcgagccgc gcctcgaggt cctcggcgta gagcgcgcgg
    28141 ccgcggatct tgatgctgtc gccgatgcgg cccaccacgt acagctcgcc gtcgtagacg
    28201 aagcccgcgt cgccggtgcg caccccgccg ttcacgaact tcgcggtgtt gacctcgtca
    28261 ccgccctcgt agccgccggc cacgcagcgt ccggtcacga acagctcccc aaggtgcccc
    28321 gggggcaggt ccgtgccgtc ctcgtcgagc acggtgagcg acaccccacg gtgggtcgag
    28381 ccgcagccca cgagccagcc cgacccgtca cccacgctcg cggagtcgcc gagcatggcc
    28441 cggtcggtca ccaccaccgg cttgcccagc tcgagctgcg accagtcgag cttcacggcg
    28501 gtgcccgtct tctggtgcga gcgcccgcac acgaggagcg tcgcctcggc caggccgtag
    28561 ccggcgatca ggctcgaggc gcgaaagccg aacggctcga gcagctcgcc gaactggctg
    28621 aacaccgcgg cgtcgacacg ctcggcaccc gacacggccg cgcgccagcc ggagaagttc
    28681 atcccgtcga gctggtgcgg cttcacgcgg cgcaggatgt gcgcgtagcc gaaggtcggg
    28741 gaggcaccca tcgtcgcgcc gcgcttgccg aagcactcga gccagcggat cgggcggcgg
    28801 acgaactgct cgggccgcat cacccagacg tggccctggt agaccgccgg cgtgagcatg
    28861 gcgccgatca agcccatgtc gtggtagtgc ggcagccatg tggcgatctg gtcggagtag
    28921 cgatcgccac ccagccactc ctccatgatc ccgatgttcg cctcgaggtt ggaccacgag
    28981 atgcgcgtgc cgcgcgggcg cccggaggag ccggaggtga actgaaggag cgcgagctcg
    29041 gcgggcttct cgacggcgaa ctccgagctc gtgggctcga ggtcgaacgc gcgtaccggg
    29101 catcgatcga cccggtcggc gatctgcacc gcgcgctcga tcagctcgag gtagcgcggt
    29161 gcggtgacga tcagcgccgg gtccgccgcc ttgacgagcg cggcgatgtg gttggcgtag
    29221 ccatcggggt cctggaggag gttcggcggc gcgagcgggc aggcgaagtt gccggtcgcg
    29281 agcgccccga agaagcacgc ctcgaagtcg ggcccgttcg gcaggatgat cgcgataggc
    29341 gcgtcgcgct cgcggcggtc cgcggcgatc ttcgccgcgg cgtcgtggac ccgcgcggcc
    29401 atctcgcccc agtcgtagta ctcccagccg ccgtcgtcgg tcgcgaagtg catcccgaac
    29461 gccgtctcgg gcgattcgag ccaaccaagc aggcgactca tgtcgcccgt tcggggtccg
    29521 ctggacgccg caagctcgta tttgccccga tgattctctc ccccgttccc ctgatcccag
    29581 gcacgaaccc acccgaccgg gcggattggg ctaggtgata caccattgac cgatgcaacg
    29641 tctcacagcg gtccagcccg cccagcgccg tcggcgggac ttggcgcgcg ggtccagcta
    29701 gctgtaagac ccgaggaggt tgttctcacc taagcgggtg acgggcggtt ggcggccgag
    29761 tgcgccgtgt ggtcgtcgga gattgtaaaa gtcgagccag ccctcgagtg cggcccggcg
    29821 ctcacggctc gagccgtaga tcctgccgta ggcccagccg tcgagcatcg tgcggatgaa
    29881 gcgctccgcc ttgccgttcg tctgcggccg ccgcgggcgg gtgcgcaggt gccggacccc
    29941 gagcgcgcga caggcgatgg cgtgggcggt cgagacgtag gccgagccgt tgtcggtcat
    30001 cacgcgctcg acctggacgc cgtgagcggc aaagaaggag agcgcacgcc cgaggaaccc
    30061 gatcgcggtg ccggcgcgct cgttctcgag cacctcgacg taggcgagcc ggctgaagtc
    30121 gtcgatcgcg acgtggcaga actcccagcc ggccttgcgc cgccgccgcc cggcggcgtc
    30181 ggtgaaggag cccgtgtagt gcgagtgacc gcccgtgatg cgcttgccgg cgccgcgctc
    30241 gatccggccg agcttcttga tgtcgatgtg gacgagctcg ccgggcgcga gcgctcgtag
    30301 cggatgacct gctcggagtc gagccggtag cgcttgccga gcccgatccg ggtgaggatc
    30361 ccctggacgg tcgtcaccgg catcgccagg acctcggcga tctcggcgcc ggtcatcctg
    30421 gtcgagcgcc gcagcagggc gatcagctgg acgcgctgct cgggcgtggc ggttggcgag
    30481 ctgtgcgggc ggctcgagcg gtcgagcaga ccgggctcgc cctcggcccg gtggcggcgg
    30541 gcccacttgc gcgccgtgcg gacgctgacc tcggcggcct cggccgcctt cgtcagcgtc
    30601 caaccgtcct cgacgcggcg aaccaggcgc cggcgcttgt tcaggctgag ctcggcgtta
    30661 ccgtggagtt tcatccggtg tcctccttgg gactggtggc ttcgacacct cccagcctcc
    30721 aagggggccc ggatgaacaa cgtcctcagg ttctagagct agccgcgcgc gcgggccgcg
    30781 gctgaggccg atccggccgc agccgtcgcg ccggcgccgc tcgcgacggc caccgcaccc
    30841 tcggcggcct cgatcgcgct ggcgaacgcg acctccgaga gcagcccgcg cgcggccgtg
    30901 tagtcaccgg ccaggaagac cccgccgccg cggtcgatgc tcgggcggtc gcgccaggtg
    30961 cgccccggca ggtcgagcgc gccggtcatc ccatccacga ccaggcgccg gcgccaaacc
    31021 gtccgctcgc gccagccctc gaagccgatg tcggcgagcg cctcgagccg ctcggtcgtc
    31081 gcgtcgggcg actcgcccgg cctgatgccg aggtggccct ggaccaggtc gtggccctcg
    31141 ggggccagcg tctcgtcggc cgcggtgtag cgctcgagcc agccgccgcc gtcgaggtcg
    31201 aacacgatga acgggtcgcc cttctgctcg cgcaggccga ggtcgagcag cacgcagcgc
    31261 gtggtgtcca cgggcagctc ctcgccgagg agcttgcgcg cacggcggac ctcggtggcc
    31321 acgatcaccg gcggctcggg cagctcctcg acctgctcgt ccgtgtggat cgcgaccccc
    31381 agctcgcgag cgcgccgctc gagcgagtcc acgagcgcct gccagccgcc ggtcaggtag
    31441 cggttctgcg gcaccgacca gagcacgcgc gcgcagcgct ggcggatgaa ctccgccgcg
    31501 agccgccccg ggtcgtggtc gaacgtgaac acgctcgcgg agttcgcgag cacgctcacc
    31561 gcgtcctcgc cgacctcgcg cgcggcccac tcggcgaagc tgaggtcgct cggggcgcgc
    31621 ttctcccgca ggcgcagtcc ctggaggaac tcgctcggcg ggacgcgctt gaggctgccc
    31681 ttgtgcacga accggagccc gcgcttgggc gagcggctgt acggcggcag caggtcgcgc
    31741 tcctcgagcc acggccagga gcggccgtcc gcgtagagca cgtgcgcgcc caggttggcg
    31801 cggaagtccc cgtcgcgggt gcgcgcccgg cctccgagag caggccccgc ctcgaaaagc
    31861 tcgaccgtgc agcccttctc ggcgctcgcg atcgaggcca cgagcccggc gagacccccg
    31921 ccgaccaccc gtatattcgc tccgcccgac acgatcgaac ggatcgtacc cggcccccga
    31981 tgaacttcgc cgagatactc acagcgtccg ccgcggcgca cccggaccga cccgcggtct
    32041 gcctcgggcc ggagcgcgtc acgtacgcgg agctgtacga ccgcgcgctg cggatcgcgt
    32101 ccgccctacg cgagcgcggc cacgaggccg gcagcgtcgt ggccgtgatg ctccagaacg
    32161 ggccggagtt cgtggcggcg tacttcggcg tgctgctggc gggcagcgtg gtgaccgccg
    32221 tcaacccgct gctgaccccg ggcgaggtgg accggctcct ggagaacgcc ggcgcgcgca
    32281 cgctgatcgg cggcgacccc gagcggccgc ccgcgccggg ggtcacccgg atcgacccgg
    32341 ccgacgcggc cgccgccgag ccgctcaagg agcccgtgcc gcgagcggac gacgacaccg
    32401 ccctgctcct gtactcgtcg ggcaccacgt ccgggcccaa ggccgccgag atgacccacc
    32461 acaacctcgt gtggagcagc gacgcgatgg tcgccgccgt cggggcccct tcggccgcgc
    32521 tctgcgtcgc cccgttcttc cagggcttcg gccagggctg cggcatgaac gggatgctgc
    32581 gcgcgggcgc gaccatccac acgcaggagc gctggaacgc cgccgcggcg ctcgacacgc
    32641 tcgagcacga gcgcgtgccc ctgctccagg ccgtccccgc gatgtgccac gacctgctcg
    32701 agctccccga cgtgcacgag cgcgacagga gcgcactgca actgtgcatg acgggtgggg
    32761 cggcgacgcc gctcgacctg ctgaagcgct tcgagcacga gttcgactgc gaggtgctcg
    32821 agggctacgg cgtgaccgag gcgctgcggg tgtccatgaa ccgcccgggc cggcgccgcg
    32881 cgggctcgat cggggtgctg ctcgaggggg tcgagcaccg cttcgtggac agcgccggcg
    32941 acgacgtggt ggacggggcc ggcgagctcg tgatccgcgg cccgaacgtg atgcgcggct
    33001 actggggtca gccggaggcg accgccgagg tgctgcgcga cgggtggctg tacacgcgcg
    33061 acctctcgta cgaggacgag gacggcttcc tctgggtgat cgggcgcgtg gacgacctga
    33121 tctcgcgcgg tggctacaag gtgcacccgg tcgaggtcga gcggctgctg gccgagcacc
    33181 cggccgtggc cgaggcggcc gtgatcgcgc tgccggacga gcggctcggc agcgagatcg
    33241 gcgcggccgt ggtggtggcg gaggggcacg agctcgacac cgaagagctg cgcgagttca
    33301 tgcgcgagcg cgtggccaag tggaagtacc cgcggcggat ctgggcggtg ccctcgctcc
    33361 cgcgcgggat cacgggcaag ctccttcggc gcgaggtggc cccgcccacg ccggacgagg
    33421 accccgccgc gtcgcgctag ctgacctgtc tgggcaggtc agctagccgc gcacctgccc
    33481 gtcgccctcg accacgaact ggcgcgtggt caggtcgcgc acgcggatcg ggccgcgcac
    33541 cggcagcttc tggaccgagt tgccgaagtc cggcccgaac ccgaggccgc gaccgtccga
    33601 gaagcgcgtc gaggcgttca cgtagacgta cgcggcgtcc acccgctgcg tgaacgcgcg
    33661 ggccgtctcg ccggacccgg tcgcgatcgc ctcggcgtga cccgagccgt acctcgtcac
    33721 gtgctcgacc gcctcgtcga gcgagtcgac cacccccacc gcgaggatcg gcgactggta
    33781 ctcggccgcc cagtcgtcgt cggtcgcggc gtccgcgggc acgccggcgg cgcgcgcggc
    33841 gtcgatggcc cgtgtgtcgc agcgcagctc gacgccgcgc ccgtgcagct cgctcagcgc
    33901 gcgcggcagg aagccctcca gcgcgccctc gtgcacgagc atcgtctcga tcgactcgca
    33961 gcgtcccggc ctgctcgcct tcgagtcgac ggcgacggcg gtcgccagct cggggtcggc
    34021 gtccgcgtgc acgtagaggt ggcagcagcc ctcgatcgcg tacacgaccg gcaccgaggc
    34081 gacgccgcgc agcaggcggt ggagcggatg gccgccgcgc tgcttgagca gcgccacggt
    34141 cgcgtgctgg gtggccagct cgcgcaggga gtcgtcgtcg gtcgccggga cgaaggtcac
    34201 ggtggccgcg gggaggccgg ccgactcgac cgcggcggag gcgagctcgg ccagctgcgc
    34261 gttcgcgcgg cgcgtggggg ctgttccctt caggacggtc gcgttacccg cgcgcagcgc
    34321 gatcgcggcc gcctcgatcg ccgtgtgcgg gcgtccgtgg aaggcccagg ccaccacgcc
    34381 gagcggcatc cgccgctcct ccacgcgcag cccgttcgcc agctcccgca cctcgatcgg
    34441 gtcggccaca gggtcgggca gctccgccgc ctcgcggcag taatccgcca gcgcgcgcac
    34501 gcggcgcgcg tcgagctggg gctcgcggaa cgcgctcgcg cccgcctggc gctggtcctc
    34561 ggcgtcggcc tcgctcgccg ccaccagcgc gtcggcggcg ccgggggcct cgagccgctc
    34621 ggccatcgcg aggatcgcgc gcgtgcgcgc gtgcgccgag actgcgacga cggagtgcga
    34681 ggcctccctg gccgcgaggc acaggtcggc caccgtggaa tgcgcgaccg ccatggggcc
    34741 cgtgagagta tccgtgcacg ctcagccgtg atgtagcgcg aaggccgctt gcgctctaga
    34801 gtcccggcgc gggggtgtcg agctgagcac tgagcaagct gacgtcgcgg tcgtaggcgc
    34861 cggtgtggcc ggtgcggcgg ccgcgtgcgc gctcgcgcgc gcgggactgc gcacggtggt
    34921 ctacgagcgc cgccacctcg atcgcgacgc caaccggggc gacgccctgc acgccgacgc
    34981 ggtggcgcgc gtcgagtcgt gggacgcgct gcccgcgctc gagaaccgcg gggccttctg
    35041 ggtgaggcgc tcggtcttca ccacgccgcg cggcgcgccg cgcctcgaga tgccgctgaa
    35101 cggcggcgcg ctgctcatgc tcgcgcacgc cgacatcgag ctggggctgg ccgagaccgc
    35161 cgagcagtac ggcgccgagg tgcgcgacca ggccgtgaag gaggcgcacc gcgacggcga
    35221 cggctggcgc gtcgtgacga agaccgggga ggagctgcgc gtgcgcctgc tcgtcggcgc
    35281 cgacggcgcg cgctcgctgg tgcgcgaggc cgccagcatc ccggtcgagt cccaccagtt
    35341 cgggcagagc accgtggtgc tgcacgcccc gctgccggac tggctcgacg acgagtgcgg
    35401 cttcgcgacg atccaccccg acggcggcgt gctgatcctc cccaccaccc cgcgcgggct
    35461 ctgccgcgtc gtggcgcagg tggacgacga ggacctgccg cgctggcgct cggcggaccc
    35521 caccgagctg cgcgagctgc tgggagcgcg tgactcgcgc ctcggcgagc tcgacgtgca
    35581 gcgctacacc ggctcgcacg tgtaccgcct cggctggcag cacgcgcagc gctacgtcgc
    35641 gcccgggctc gccctggtcg gagacgccgc ccacgtgaca cacccgaacg gaggtcaggg
    35701 aatgacgctc gcggtctatg acgccgccgc gctcgggcag gtcgtcgctc cagcgctgct
    35761 gaacggcaac ggcaacggcg acggtcgctc gctcaccgcc gcgctgtggg agtacgcggc
    35821 ccggcgccgg atgccgagct gggagtcgct cgagcgcgcg aaccgcgtgg cccgcttcca
    35881 gcgcaagggc acgctgccct acgccggctc ggtcgcgatg ctcggcctgt tctcgatctc
    35941 cccttcggtg accgcccggc tcgcacggag gttcggcggc gaatgagcga ccggcgcgta
    36001 gcgatcgtca ccggagcatc gcgcgggatc ggcgaggcca ccgcctggcg gctggccgcc
    36061 gccggctggc acgtgttcgc caccgcgcgc gacacggcgt cgctggaggg catggcgtcg
    36121 gagcgggtga ccccgctgcg cctcgacctc aacgacgacg actcgatcca ggattgcgtc
    36181 aacacggtgc tcaccgacgc gggccgcgtc gacgccctcg tgagcaacgc cggctacagc
    36241 gcgtcggggg cgctcgagct gttcgagctc gaggacgtgc gcaggcagtt ccagaccaac
    36301 gtgttcgggc cgctgcggat gagccagctc gtgctgcccg cgatgcgcgc gcaggggagc
    36361 ggccggatcg tgcacctggg gaccgtgatg gggcgcgtct cgttccccct gctcggcgcc
    36421 tactcgagca gcaaggccgc gctcgagtcg ctcaacgacg cgctgcggct ggaggcccgc
    36481 cgcttcggcg tgcgggtggt catggtgatc cccgggaccg tccgcacgga gttcgaccag
    36541 gtcgccctcg acacgctcgc cgagcggatg cccgacgagg gatcgccgca ccgggagtcg
    36601 ctcgagaagc tcgagaagat gatctcggac acgagcgaga agggcatccc gccggaggcc
    36661 gtggccgcga aggtccagga ggcgctcgag gcgcgccacc cgcgcagcgt ctacgtgctc
    36721 acgccggagg cgcggctcgc gatgcgggtg ctcgcgcggc tgccggtggc ggtgcgcgac
    36781 ccgctgatgc ggatgccgct ccgcctctga cgggcggctg cggctccgcc tcgaacgcgc
    36841 ggccgcgctc gagcgcgcgg ttccgcctcc gacgcgcggc tgcgctccag cgcgcggtcc
    36901 gcctccgacg cgcggctggc cagctccgcc tccggcgcgc gactcgctca ggtccagcga
    36961 cccgagccga ccggctcggt gcccggcgcc acctgctcgc gcagcttctg gcgctgcacc
    37021 ttccacgtgg ccgtgcgcgg cacgtccgac cacggcagca cgaccgggtc ggccagctcg
    37081 ggcaggtcgg ccacggcacg cttccacgcg tcgggatcga gcggctgccc gtccgccatg
    37141 ttcagcacgg ggagcggcgg cccgccgttg acggccagca tgaccgcctc gaccagcgac
    37201 ggcatccggt cgagcagcac gtcctcgagc tccaggcagc tcccctcgtc gatgcggtcg
    37261 acctcgcggt cgaggagctg cacctcgttg gtgcgcgaga cgacgccgag atcgcccatg
    37321 ttccaccagc cgtcgttgcg cttgctctgg aagcgatcgt cctcgccgac gtagccgagc
    37381 gcgcagccgc gcttgtggac ctcgatcagc ccggcgcgcc cgcggccgag cggccggccg
    37441 gtgtcggggt ccaccgcccg gaccttggtg atgaacggga ccgggcggcc gaccttgcgc
    37501 gtggccggca tgcgcccgcc gcgatcgcgc acagaccggc gcgtgtagat gccgagcgtg
    37561 accggaccgg cctcggtctg gccccagccc tggacccaca gcgggaagcg ccgcttcgac
    37621 gcgttgagga gcgtgcgcac gctgcggacg ttgaccgcgt cgaaggtgtt gatgaacagg
    37681 cgcacgtcgc ggaacggctc gccgggatcg tcggccatcg cctcccacag caggaacgcg
    37741 ttcggcacgg tctcgatcac ggtcggccgg tgctcgacca ggaggtcgcg cacgttcggc
    37801 gcgacggggt cggacagcat cgtcaggcgc gggccgttct tggtcacggc cgcgaggccc
    37861 gtgaacacgc gaccgtgcac gaacgggtcg cagaagagca cggagtcgcc gcccttgagc
    37921 ccgaggaccg gccagcgctt cgactcgacg gccgacagcg cgcgcagcgt ggccgccgag
    37981 tgggcgacga gcttcggcgt cccggtggta cccgacgtgt gggtgatgac catcgcctcg
    38041 tcgtcgggtc gcggggtgag gggcgggatc gacccgccgc gcaggtccgc gagcgccgtc
    38101 gttgacccgt tggaccccgg ctcggcgtcc accacgatga tccgcttcac gagcttcgcg
    38161 acctcgtccg agccgagccc gcacgactcc atccgcgccc ggtcggtcac cagcaccggc
    38221 cggtcgaggc gcgccagcag ctcgcgcgcc acctccggcg agtgggtgca cgcgatctgc
    38281 gccggcaccg ctccgatgcg cgccgccgcg caggacagcg cgacgatgtc ggagtggttg
    38341 gccttgatga tcgctacgcg atcgccggac tcgacgccgc cctcgcgcag ccagctcgcc
    38401 acctccctga cgaactgcgc ctggtccgag aaggtcgcga tgtcgcggtc cggatcgatg
    38461 tccggcgggc ggtcgccggc cgtcctctgg tccgggtaac gctcggccgc gtactcccac
    38521 gctgtcgcga tcccgcgcga gaacgggccc ttcgctcgtg ccccagcccc cgccagacgt
    38581 cgcatgcagc ccctctcagc tccgattgca aggcagtctg gttattatgc cgaccgaacg
    38641 cccggcgggc gcggagggga cgggggactt tggctgaggt cggagaggca gcgcgcggcg
    38701 cggacgtgct cgacgtgggg cgggacggcc ccagcgcggg cccgaccacg cacggcttca
    38761 aagagcgcct catcgcgtac aaggacctcg cgaagctcga gatcttcgac gtctacctga
    38821 ccgtcccgct cgccgcgtcg ctgctcaccg ccgaccagtt cgggaccgcc gagagcatcg
    38881 cgatcctcgt gctgatgctg ttcacgcaga tcggcgtcgt caccgcggcg tgcgcgctag
    38941 acgacgtggc cggcatacgc gacggcatcg acacctacac ctacgggcag gacagcaacc
    39001 tgcgtccgct caagcgaaag ccactggtaa acaacaggct gaccgaggct caggcgatcc
    39061 gctacgcgta catcgcggcg ggtgtcggcg tcgcgtgcgt ggcggcggcc tacgccgtcg
    39121 ccgacttcga gccgcgctgg gcgccgctca tggtggtcgc gatcgtcacc tgcgtcctct
    39181 tctactcgtg ggggccgaag ctgagctaca tcggcggcca ggagttcgtc gtggtcctgg
    39241 gcgtgacgtt cacgctggcc ggcacctacg ccctgctcac cggcgggatg acctggacgg
    39301 tcgtgctcga gggcctgctg ctcgggatct ggctgatgca gctcaccgcc ttcgcgaacc
    39361 agcacgaccg cgagggcgac aagcgcgcga accgcgtgac catggccgtg aggctcgacc
    39421 cgtccacgta caaccgctac ctcacgaccc tctacgtgct cagcctcgtg acgctcgcga
    39481 tcgggctcgt gaccgaggag ctcccgtggg agctggcgcc gctggccatc cccgccctcg
    39541 tcatccagtg gatcgcgttc aacgcggggg tcatgcgcgg cgacgggctg cgcgcccgcg
    39601 cgctgagcct cacggtcctg cgcgccggct gggtcgccct gttcgtcgcg aacctggtgg
    39661 cctagaggag ctccgtgggc gatctgctgc tcaagctcga cgggtggctc actcgccgtc
    39721 gcaagctggt gatcgggttg tgggtcgccg cgctggtggt cgcggcgccg ctctctgcga
    39781 tccagagcac cgcgctgacg gagggcggct tccaggtcga ggggagcgac tcccacaccg
    39841 ccaaccaggc gctgcaggac gacttcggcg agcgccagcc gaacgcgctc ggcgtggtgc
    39901 tggtgcgcga gggggcgagc ccgcaggagt tctcgaagtc cgtggacgag tacgcgaaga
    39961 tcgccgccgg cgtggacaac atcgaggtcc cgcccgcggc ggtcgagtcc gcgcgcacgc
    40021 gcgcgctcac cagcgacaag gtgctgttcg cgctcacggt gatcggcaac caggacgaga
    40081 gcgccgacgc cgcagtcaag ctccgcgacg acctgaaggt caaggagggc gcccgcgacg
    40141 gggtgaggcc gtacatgatc ggcgcgatgg cgttctgggc ggcgctacac gagcttcaga
    40201 agaccgacgt ccagaaggcc gagcgcgtcg gcgtcccgat cacggcgctg atcctgctcg
    40261 cgaccttcgg caccctggcc gccgcggcgc tgccgctgct gctcggcatc gcgagcgtgg
    40321 tcatcaccgg cgcgctcatc ttcctcgtcg cgcaggcgct cccgatgtcg gtctacgtga
    40381 cgagctcggc atcgatgatc ggcatcggag tcgcgatcga ctactcgctg ttcgtgctcg
    40441 cgcgctacag acaggagatc gcgtccggaa ggaccgagga cgacgcccgc cgcgaggcgc
    40501 tgaggacctc cgggatcgcg gtcgtgttct ccgggctcac ggtgatcgtg tcgctctgcg
    40561 cgctcctcgc cttccccacc gtcgcgctgc gctcgatggc gctcggcgcg atcatcgtcg
    40621 tgtcgatggc gatgctcgcc gcggtcacgc tgatgccggc cgtcatgacc atcctcggcc
    40681 cgcgcgtgta cgagccgggc cggaccgccg tctggcgcga ccgcatcgtc gggcgcttcc
    40741 gccgcgaggg cggcgacttc tgggggacct ggacgcggcg cgtgatggcc cggccgatgg
    40801 tgaccgcgat cctggccgcg ctcgccctgc tcgcgctggc gatacccgcc ctagacgtcg
    40861 agatccgcga ggagtcgcgc tcgcagctgc cggccggcca cgacgccatc caggggctga
    40921 accaggccgc gccgctgatc gggccgggcg cgctcgggcc gttcgtcgcg gtggtccggt
    40981 tcaactcggg caccgccaag acgcccgcca accagcaggc cctgtcggcc tactcgtccg
    41041 ccgtcgagag cgaccgcgag gtcgcgtacg tcacaccgcc gcggccgtcc gaggacggtc
    41101 gccaggcggc gctgacgatc gtgccacgtc acttcggcga gcaccccgac gcgcaggcgc
    41161 tgctcggccg cctgcgagcc gaccgcggcg gcagggcgct ggcccccgtc ggctcggtca
    41221 gcttcggcgg cgacgtggcg atggtgcacg acttcgctga caacgtcgcc gacggcgtct
    41281 tccgcgcgtt cctgatcctc gtctgcgtga cgttcgtgat catgatgatc ctgctgcgct
    41341 cgatgctgct gccggtcaag gcggtcgtga tgaacatcct cacggtggcc gcgaccaccg
    41401 gcatcatcgt ggggatcttc cagtggggct ggctcagctg gaccggctac gaggaaccgg
    41461 gctacatcgc cgccgaggtg atcccgctga tgatggccgt cgtcttcggg ctgtccatgg
    41521 actacgaggt gttcatgctc acccgcattc gcgaggcgtt catgcgcagc ggcgacaacc
    41581 gcgaggccgt cgcgaacggc ctgcgctcga gcgcgagcac gatcaccagc gccgcgctga
    41641 tcatggtgtc ggtcttcttc gccttcgccg ccgtcaggat cccgtcgatc cggcagatcg
    41701 ggaccgcgct cggggtggcg gtcgcgctcg acgcgacgct cgtccggctc gtgctcgtgc
    41761 cggcgacgat gggcctcctc ggtcgctgga actggtggct tccgacctgg ctccaccgca
    41821 tgccgcgtgt cctgcccgag gtggtaccgg aatcgggagt gtccaagggg tcctgaagtt
    41881 ggccgtcggt acagaggggc aggggtcgta cgcgctgttc gggaacgtcg gcgacgagca
    41941 gctgcgccgg ctgaagtcct cgtccgccgt ctgggacgag ggaacgctcc gcttcctgat
    42001 gggcctgggg ctcgccacgg gctggcgctg ctgggaggtc ggtgcgggcg agggctcggt
    42061 ggcgcgcgcg atggcgcagg tcgtcggccc gaccggccac gtggtcgcca ccgactacga
    42121 cccccacttc ctcgtgagct cggcggccca gaacctcacg gtcatcgagc acgacgtggt
    42181 gaccgaggat ccgcccggcg aggggttcga cctcgtgcac gcgcggatgc tgctccagca
    42241 cctgcccgac cgcgaggagg cgttccgccg gatggccgag tccgtcgcgc ccggcggctg
    42301 gctcgcgctc gaggactccg actggatcac cttcctcgtc gcgcgtccgg cgattcccga
    42361 gctgcgcgtg ctcgcgagcg cgctcgacgc cgccatggcc gacgcgggct tcgacagcac
    42421 ctgcggcttc ggtaacgtca cgcgcctgaa tgactccggc ttcgaagacg tgcgcgggca
    42481 gggccatgta ctcgtcatgc gcgggcgctc gaccggcgtg acctggtacc accagtgggc
    42541 cgcctccctg cgcgactcga tggtcgcggc gggttacctc accgacgacg agttcgagcg
    42601 cgccgacgcg gtgctcgacg accccagcgc cgcctggctc tcccagacgc tggtcgcggc
    42661 ctgcggccgc aagccgtcca agtagcacct gagcggatgg ccacacccga ggagactccc
    42721 gaccagggcg cgtacgctct gctcaccgag gtggaggagg tcgaccaggc gcgcatgctc
    42781 ctctcggccg cctcggtctg ggaccaggga acgctccggc gcatgtgcga cctcggcatg
    42841 accgagggct ggcgctgctg ggaggtcggg gcgggcgagg gctcgaccgg gcgggcgatg
    42901 gcacgggtgg tcggcgagtc cggacacgtc gtggtgagcg acatcgacac gaccctgctc
    42961 gacaccgccg gcctggccaa cgtagaggcg gtccagcacg acgtggtcgg cgacgacccg
    43021 cccggcggcc ccttcgacct ggtgcacgcg cgctgcctgc tgccgcacct ccccgagcgc
    43081 gaggaggcgt tcgcgcggct ggcctccgcc gtcgcgcccg gcggctggct catgctcgag
    43141 gacaccgact gggccacctt cctggtggct cacccgccga tgcgggagtt cgagctcctg
    43201 cgcgcggggc tcgaggccgc gatggccgag ggcgggttcg acaacacctg cggcatgggc
    43261 aacttccacc ggttgcgcgg cgccgacttc gaggacgtgc gcggggaggc ggaggcctcg
    43321 gtcatgcgcg ccggctcccc gggcgtcacc tggtaccacc actggtcggg ccggctgcgc
    43381 gagcagatgg tcgccgcggg gcacatcacg gacgccgagt tcgagctggc gaacgcgcgc
    43441 ttcgacgatc cggacgccgt ctggctgtcg cagacgatga tctcggcgtc gggccgcagg
    43501 ccgctggagt agccggctca ggtccagcgt ccggcgcccg ccgtggcggt ccccggcagc
    43561 aggtgctcgc gcaggacgtg ccgcctgacc ttccaggtgg cggtgcgcgg gaactcgtcc
    43621 cagcggatga agacgggatc ggccagcggc ggcatgccgg cggtcgcggc gctccaggcg
    43681 gcgtcgtcga tcggctcgtc gtccgcggtg ctgacaaccg ggacgggcct gccgtcgcgc
    43741 accgcgagca cgatcacctc ggtggccctc tcgagccgct cgagcagggt gctctcgacc
    43801 tggatgcagc tcgcgtcggc gatccggtcc acctcgcggt cgatcagccg gatcgtgccc
    43861 gagcggttga cgatcccgag gtcgccgagg ttccaccagc cgtccctgcg cttggccagg
    43921 tagcgctcgt gctcgccgac gtagtcgagg cacgggtcgc ggcgcacctc gagcagcccg
    43981 acgttgccgc ggccgacggg cctgccggtg tccgggtcga ccgccctcac gcgggttagg
    44041 ggggagacgg ggatgccgcg cgcctccgcc acccggcggc cgcggccggg ccggacggag
    44101 cggcgcgtga acaggcccat ggacacgggc ccggactcgc tctgcccgag cgcctgcacc
    44161 cacacgggga agcggcgccc cgacgcattc agcaggacgc gcacggtgcg ggtgtggatc
    44221 gggtcgaacg tgttgaagta caggcggacg ttgcggaacg gtccggccgg gtccgcggcc
    44281 agcggctccc agctcaggaa cgcgttcggc gcgctctcga cgatggtggg cgcgtgcttg
    44341 accagcacgt cgcgtaccgc ggtcgggtcc gggtcggcga cgagcacgag cttcggtccc
    44401 gagcgcgccg cgccgaccag cccggtcgtc aggcggccgt ggaagaacgg gtcggcgaac
    44461 gcgacggtgt cctcgcgtcg cagcccgagc accgggacgc ggcgcgtctc gtagcgcgcg
    44521 gcggtggcga ccgtgcgcgc cgagtgcacg acgagcttcg gcaccccggt cgtgcccgag
    44581 gtgtgcatga tcaccatcgg gtcgtcgtcg gcgcggacct ggacgggggg cgcgctcgcg
    44641 cccttcagcg agggcagcgc gatcacgtcg tcgccgaggt cgtccacctc gtcctcgaca
    44701 aggagcgtcc tggaggtcag cgcgcgcagc cgctcggcgt cgagctcccc cgcgcgcagc
    44761 cgctcgcgat cggcgatcag cacagggcgg tcgagccgcg agagcatggt ggccagcacg
    44821 tcgggcgggt gcgtccacgc cagcggtgcc gggatcgcgc cgaggcgggc ggccgcgcag
    44881 gcgagcgcca cgacgtccga gttgttgccc ttgaagatcg cgaccctgtc ccacgggcgg
    44941 acgcccgcct cgtgcaggcg gccggcgagg tcgcgcacga actccgccca gctcgcgtac
    45001 gtgccctcca gcggccgcgc cggatccacg tcggacgcgc gcgtgtcgat cacctgctga
    45061 tcgccgaccc gctcggcggc gtcctcgagg agcgtgccca accgggccat ccgggcatcg
    45121 cgcgtgcgtc cgagcatcag cgtgccgcac ccgctcggcg acggcgccgg cgcggccgcg
    45181 tccggctcgc cccgtccgag ccggcgatca ggcgccgccc gaacctgcgc tcggccgcgt
    45241 gcaccgggcc gagcgcgccc gcggcgcccg cgcgctggat catcgccgac atgcgcgaga
    45301 gcgcggagct ctcgctcgcg tccatccccg cccggatccg ctccgtgcac cagtccaccg
    45361 tgtcgccgag ggtctcgtcg agcgggcggg cgcggtagcc gaggtcgcgc tcggccttgc
    45421 gcgagctgaa gcgcgtgttg ccgcccatca gcgtgagcgt ctcggccggc atcggcatcg
    45481 gcaggcgcgc tcgctcaccc gcgcgcgccg ccgtcgcgaa cgcgggcggc agcacgaaga
    45541 ccgggtgccg cacgccggag cgctccgcga gccgctcgat caggtccgcc cacgcgatgt
    45601 cgtggccgcc gagcacgtaa cgctcgcccg gccgcccccg ctccgccgcg agcacgtggc
    45661 cctcggccac gtcgcggacg tccacgaggt tggtgtcgcc gcccaccacg gcgggcagcc
    45721 gcccgcgcag gtactggcag accacgcggc tcgatccgcc cgagtcctcg ggccggaccg
    45781 gcgggcccag cacgtagccc gggtggacca ccacgacctc gacgcccagc ctgcgaccgg
    45841 cctcgagcgc tccccggtcg ccctcgcgct tcgactcggg gtagacgagc ggcacgcgcg
    45901 actcgacgcg gtcctcctcg tcgcccgtgc ggcccggcgg agcgaacccg agcgcgagca
    45961 ccgacgaggt cacgaccact cgctcgatgc cggctctcgc ggccgcctcc accacccgcg
    46021 cgggcccggc cgtgttggcc tcccacgcgc tgcccgacgg cggcgagccg acggccccgg
    46081 cgcagtgcac gatcaggccg gtgccgcgca cggccgcgtc gatcgcgtcg cgatcgagca
    46141 cgtcggccct gaccggctcg gcaccgagct cgcgcagccg gtcctcaccg ccgccgcggt
    46201 acgtgccgcg cacctcgtga ccacgctcga ccatgaggcg caccacgtgg gcgccgacga
    46261 acccggcagc ccccgttaca cagacgctca ggcccgctga catgctctcc ccccgtcgcc
    46321 ccagatgagg gcgcacaagc tactcacttc acccgagcgc gacgaggttg gcgcagaaga
    46381 gcccgagcca gccgacgcgg taggcgaggt ggcagagccg ccgggccagc aggggctcgc
    46441 cgcgccagag accgtcgcgc agcgcgcggt gctgaatcgc gaagcagggg accaggaaca
    46501 ggacgaccca ccagtccagg gcaccgagga cgatgccggt gaggaggacc gtccagccga
    46561 gcgcgaacag gccgaggatg tagcggcggt tgccctcggc cgagagccgg acggccatcg
    46621 tcgcgcgccc tgcctggcgg tcgccctcca ggtcgtggag gttggcgaac gtcaccggct
    46681 gcatgagcca tatcccgagc agcaggccct cggtggcgac cgcgccgctg aggctcccct
    46741 cgatcacggc gtaggtcccg tagatcgcca ccgctagccc cgagatcacc acgatctcct
    46801 gcccgccgcg gtagctcagc ttcggccccg ccgagtaggt caccacgagg aacgccgccc
    46861 ccgccgcggc gaacggcacc cagagcggct cgaagtcggc gatcgcgagg ccggcgacga
    46921 acgcgatcgt ggccaccgtc gctgcgaagt acgcgtagcc cttggcctgc tgctcggtga
    46981 gcctgccgtc gagcagcggc ttgcgcgcgc gcacgcggtg ctcgctcggc gcggcgtagt
    47041 tcacgacgtc gatgccgtcg cgcacgccgg ccacgtcgtc gagggccgac tccacggtcc
    47101 ggaagccgac ctgggtcacg aggctcaggg cgagcaggcc gagcgcgtac ccggacgtcc
    47161 ggtggtccgg ctcgagcgcg gaccagacga gcgggacgga caggaacgct ccccattcgg
    47221 tgagcttcgc gaggtccttg tacgcgagca gccgttcagt cagcggcacg gtcccccgtc
    47281 agctcgcgct tcaggacctt gcccgtcgcg ttccgtggga ggcgctccat gaagacgacg
    47341 tcgcgcggca ccttgtagcg cgcgaggttc tcgcgcacgt ggtcgcgcac gtgatcctcg
    47401 gtcaggccgg cgccgggacg cgcgaccacg aacgctcgca ggcgctggcc gaactcctcg
    47461 tcgggcacgc ccgtgaccgc cgcctcctcg acggaggggt ggcgtgcgag cacctcctcg
    47521 acctccgcgg ggaagacctt ctcggcgccc gagatgacca tctcgtcgtc gcggccgtcg
    47581 atcagcaggc gcccgccggc gtcgatgtgg ccgaggtcgc cggtcgccat catcccgtcg
    47641 atcacctcct tgccgccgcc gcccgtgtag ccctcgaact ggacggcgtt ggagacgaag
    47701 atccgcccgg tggtcccggc gggggcctcg cgcccggcgt cgtcgagcac cttcaccgac
    47761 gtgccgagcg gcgggcgccc gacgcagccg ggtgccgcgc gcaggtcatc ggggtccgcg
    47821 atcgtggcct gcgcgacctc ggtggacccg tagacgttgt agagcacgtc gccgagcacg
    47881 ccgctcgcct cggcgcacag ctcggccgac agcggcgagc cgccgaccgc cacgacccgc
    47941 agcgccgcca ggtcgcgccc gcgcacctcc tcctcgccga gggccagcag ccgccgcagc
    48001 atcacgggca ccacgacgag ggtggtgcag cgcgtgcgct cgagcgtgtc gagcacggcc
    48061 gccgcgtcga agcgccgccg gagtaccagc cgtgtgccga ggccgagcgt gagcgacgcg
    48121 gtcaggaggc cccacgagtg gaacagcggg ggcgcgacgt aggtgacccc gccggcgcgc
    48181 agcgggatcc gcgacaggag cgccgcgggc acggtcagag tcttcggctg cccgcggttc
    48241 gcgcccttcg gcgtaccgct cgtgccgctc gtgaggatca cgacgcggcc cggccgcttc
    48301 ggcgccggga gctccgaccc gtcggccgac gcgatcagct cctcgatcgt gggctcgccg
    48361 ggagggccct cgggcagctc ggtccacgcc cgcactccct ggcggccgcg gcgggcgtcc
    48421 gccgtgcgct cggcgaactc gtcgtcgtag acgagcgtcg tcgcaccctc gcgctcgagg
    48481 atctcggcga gctgcgggcc ggccgagtcg gtgttgagga agatcgcgcg ggcgccgtgc
    48541 ttcgccgacg cgaacgcggc gtcgaggatg aagcggtggt tgcggcagag gatggcgacg
    48601 ccgtcatcct gcctcacgcc gagccccccg agcgcgcgag cgagcgagtt cgaccgcgtg
    48661 tggagggccg cgtagtcgag ctggccaagc tcgtcgtcga gggcgatcgc gctgccgtgg
    48721 cgcagggcgc cgatccggga gatcgcgccg atctgcccga acgcgcgcat cgcccggagc
    48781 atctggagga ggtggtccgg gcgcgcgagc ggcagcatcc ccgcgcgcgc gaggaccacg
    48841 aaggacctga cctcgttgcc cgccgctcgg gcgagacgtc gtgcggaagc ggccgtgagc
    48901 atggcggcgc gggattcgat cacatcgcgg ggcggggcgg ggagccgtgc cgaagatggc
    48961 gcgttccgta tcctccgcgc gcacatgagc gtcgccccgc ggacgcccag gacccggccg
    49021 ctcaaccgcc tgcgcctcgt gttggaccct gcgctcacgc cggcgaacgc gctggacaag
    49081 gcctgcgacg cgcatccgga ccgcgcgatc ttccaccccg acgcggactc cggcttcgcg
    49141 gcgctgcgcg ggcgggcgat caccccgcgg cagctgctcg tcttcgccgg cgggctcggt
    49201 gagctgctgc gcgacggcat gggcatgcgc gcgggcgatc gcgtcgcggt gatcaagacc
    49261 aaccacgtgg actacttctt cctcgtgctg gcgatcgtcc gggccggcgg ggtcgcggtg
    49321 ccgatcaacc cgggcatccg cggcgagcgg ctggagcgct acctccgcca taccggcgcg
    49381 cggatcgtcc tcaccgacgc gccgacgttc gcgagctgcg tgggcgaccc cgagcgcatg
    49441 ccgtcggtcg agcggtgggg cttcccggcg gcgccggcgg ggttcggggc ctcgcacgtc
    49501 tcgatcgact ccgagctgcc ggccgcgggc gcgccggcgc cgcccgcgcc gctgcgccgc
    49561 gtcgacccgg tggtgatcgc ccacacctcc ggcacgaccg gattcccgaa gggcgtggtt
    49621 agcacgtccg gaacgctggc ggccgggatc aagggccact acgtggacga gccggtcgcc
    49681 accggcaacc gcaccgggat cgcggggcac ttcaaccacc tcgtctacca gagcggcctg
    49741 ttcgcgaccc tgctcagcag catgcccgtg tggactctga gcccggacga cgcggccggg
    49801 gcgctgcggg cgatcgagcg cgagcggctg aacttcttct tcgcgttccc cgacgtgtac
    49861 ctgcgcatgt accacgaggg actggcgggc cacgacctgc gctcgatgcg gctctgggtg
    49921 acgaccgccg acgcctcgca cgacgtgcac atgcgcgcgt tctgccgcac gggcgcgtac
    49981 ctgcgcgtgc tcggcgtgcc gcttatgcgc tcggcgttca tcgaggtgct ggggagcacc
    50041 gaggtcgggt ccgcggcgct gcggcggatc cggctcccgt tcgcacggcc gcgctccgac
    50101 cgcccgatcg ggcggcggac cccgggcggc ccgcgcgtga ggatcgcgcg cgaggacggg
    50161 cgcaccgtgt ggcggccggg cgcggtcgcg cgcgtggagg tgcgcggccc cgccgtcttc
    50221 gccggctact gggacgaggc ggcgatgcga ccggcgccgc gcgagcgcgc ctgggcctgg
    50281 accggcgacg tcggctaccg cgaccggctc ggcgtcttcc accacctcga ccgcgcggcg
    50341 gacgtggtcg acacccgcgc gggaccggtc tacacgctcc cggtggagga ggtgctgctc
    50401 gagcatccgg ccgtgggcga ggtggccgtg atcgggctgc cgcatccgga cggcggtgag
    50461 ctgccggtgg ccgtggcgca cgcgcggccg ggggcgacgc tcgacgccgg cgagtgcctg
    50521 cgctgggcgc gcacgcagct gacgctcgcg gcgccgctcg cggaggtggt ggtcgtcacg
    50581 ccggaggaga tgccgcgcgg gctcaccggc aaggtgctga agcgcgagct gcgcgagcgc
    50641 tacgcggacc gcctcgcggc gccggcgggc gcggcggccg gcgagctagc cgatcaggcg
    50701 ggcgccgcgg cgcaggagtg acggcggcag ccggtgctgg aagcgcagcg actggtcgcc
    50761 gaggtcgtac acggccggac gcagcctccg ccgcagctcc ccgagcgact cgtcgagcac
    50821 gtcgagcacc cgctcgatct cgtcgcgctc gaccgtgagc ggcgggtaga ggcgcagcac
    50881 cggggtgcgc gcgaccgaca gcgacagcgc ccccagaact ccacgcttga ggagctgtag
    50941 ccagagcgcg ttctcgacca cgcggttgcg cagccgcagc ccgaccatca gtccgcggcc
    51001 gcgcgcgtcg tgcacgagct ccgggtggga ccgcgcgatc cgctcgagcc cgtccagcac
    51061 caccggcccc agcgtcgcgg cgcgcccggg gaggtcgtgc tcgacgatga agtcgagggc
    51121 ggcgttggcg gccgcgcaca caagcggcga gcccgccgac tcggacgggc gctcggcggc
    51181 ggcggcggcg agcgcgtcgc tgcccgcgag caggccctcg cggacggccg tgacggcgat
    51241 cggtgtgtgg cccccggtga tcgccttcga caggacgatc atgtcgggca cgatcccgtc
    51301 gcgctcgacg gcgaacatcg tgccggtgcg gccgaagccg gtctgcacct cgtccaccac
    51361 gagcagagtg ccggtcgcgt cgcagtgctc gcgcagcgcg gcgaggtagc cgtcgggcgg
    51421 caccgagagc gtgcctccgc caaggatcgg ctcgaccacc atggcgagca cgccgtcgcc
    51481 gatcgcctcg gcggccgccg ccgcgtcgcc gtgcgggacg aacgacgtgc tcggccacga
    51541 ccggccgaag ggaacgcgca ggtgctcctg gcccatcacc gcgagcgctc cgagcgtctt
    51601 gccgtggtag ctgtcggacg cggcgacgag ccgcctgcgg tccggccgcg ccaggcgcgc
    51661 ggtacgcagt gcgatctcca tcgcctcgga gccgcttccg gccagcgcga tccggtcgag
    51721 gtcgccgggc aggagctcgg cgagcctgcc gatcagcgtg gcggccggcg cggtgcggag
    51781 cagcggcggc gcgctggcga gcgtgtggag ctggtcgagc acggcggcgc gcacggcgcc
    51841 gttgccgtgg ccgaccccga agacgccgtg gccgccggag aagtcgaggt actcgcagcc
    51901 ctcctcgtcc tggaggaccg cgcccgaccc cgccagctcg accggcgccc cgtaggcgtc
    51961 gaacatccgc gcgaggctcg ggttgtagtg gctgcggtag gcgccgagca gccggtcgcg
    52021 catcaggccc gcaccgccgc ggtctccgcc gcgcccgcat ccgcatcctc gaccgaccgc
    52081 agcgctcgct cgagtgcggc gagcccgcgc tcgagctcct ccgccccgat cgcgagcggc
    52141 gggcgcagcg cgatccatcc ggcgcccgtg ccggtggccc ccgcgcccac gagcgcgccg
    52201 gccgcggccg ccgcgtgctg gagctcgtac gcgaggtccg ggtcgcgcac gcgaaagccc
    52261 gcgagcagcc cgcagccgtc ggggtcgagc acgagctgcg gccgctcgtc gcgccagcgc
    52321 tcgagttgtt ccatcagccg ggccccgtgg ccggcgcagg cccgctcgag ctgctcctcc
    52381 tcgaccgcgt cgagcaccgc cagcgccgcg gcgcacgcca ccgggctgcc gcccgtggtc
    52441 gaggcgtgca gcaccgggtc gcgccgcccg tagacccgct ggttgacgtg ccgcgtggtg
    52501 aggtaggccg cgaccggcag cgcgccgccg ccgagcgcgc cgccgaccac cagcacgtcc
    52561 ggggccaccc cctcgcgctc gaccgcgaag cggcagcccg tgcggccgag gccggtggtc
    52621 acctcgtcca cgatcagcag cgcgcccgcg cgggtcgcca tgctccgcag ccccgtgagg
    52681 tagccgtgcc gcggcacgcg cacgccggca cccgtggcca cgggctcgac gatcaccgcc
    52741 gccgtgcgcc ggtcgatcgc gtgcgcggcg gcgtcgaggt cgtcgtacgg cacgcgcgcg
    52801 acctggacgg gcgcgtccgc gatcgtggcg cagaccgtgg ccgcgtcgct caccgcgcgc
    52861 gctccgaggg tcgagccgtg gtaggcgccc gacatggcga ccacgcgggt gcgccccggg
    52921 cggtagccgc gggcgagctt gagcgccccc tcgaccgcct cgctgccgga gttgcacgga
    52981 taggtgaccg agagcgtgcc gggcgcgacc cgggccatgc gctcgacgag ggtggcgagc
    53041 gggcggctca ggaagtgccg gctcgacagc ggcatctcgc gcatctgcgc ctcgacgcac
    53101 tcgagcacgc gccggtcgcc gtagccgaac ccgccggagg agaggtccac gagctcacgg
    53161 ccgtcggacg tgcgaacggt ggcgccggcg gcggacaccg ccacgcccgc gtcgagcgcc
    53221 tcacgccgga gcgcgcccca gggagccgcg tgctcgacgt acgcgggctt caccgaatct
    53281 tgcgcgagtg gacgcacggt agaccgcccc agccgctcga cgcgaccggg gcgctcgacc
    53341 ctctacgtgg ttgttccgga cctccgcagc tcgtccgcca cgagccgcgc cagcagctcg
    53401 tagtcgatcg gctcgtagcg gcccccgtac acgggccggt cgggatcggg ccgcagctcg
    53461 tcgaagacgc gccgcgacga gtccgcgagg ctctcgtaga actccgcgtt gccctccgcg
    53521 aacgcctcga acgtgctgcg ccccaggccc tgggcgtcca ccgcctcggg ctcgagctcg
    53581 gcgttgaagc gctcgaacga gtcggccacc gcggccccga ggctgcggta gaactcgccg
    53641 gtcgcgacga gcgtctggcg ggtcgcctcg cacgagtcgt agcccgcgcg ccggcggcgc
    53701 tcacggctct cgaacgagct gcgcctgcgg gcctcacggt cggcgaacga cgcccgccgc
    53761 gctgccgccc gccgctcgta ggcggcgctc ttctcggctg gatcggtgcc ctcgcgcgcc
    53821 gtcccgggct cgtcgcctat ggcggcgggc cggtccgggg gcgggtctcc tatcgaagtc
    53881 atctccaccc tctccggtca ctcatcgtgc gcccctccgg aactcgcgag cgagcctccc
    53941 gaggagcaga gttgcaaacg tgccgaacgc taccaccagc caaagcggtg ggccgagacc
    54001 ctcctcgagc cggtcccagc cgccgccctc gaacaggagc ggagcgaccg tcacgctgcc
    54061 gatcagcgcg agcgtggcga gggcgaccgc cgcgcgcgcg tacgagaaca ggagcccgcc
    54121 gatgcgctcg gcgcggtcgc gctgctggcg gtactcgcgg ccgacgccag ggagaccgga
    54181 ctcgacccag tcgctcagcg cggcgagcgt gcgcggggcg tcgacggtcg cgcgcacggc
    54241 ggcgttgatc gtcgccggcg aggtgatgcg cccgaggcgc tcgccgctct ggcgccacag
    54301 ctcgtcggcc acgaagcgcc gcacgatcgg gacccagtcg atgcccgggt ggagcttgag
    54361 catcacgatg tcggccacga agatcgagcg gtagaggcgg accagcccgg tggggagcct
    54421 cagccccgcg cgccggacgg cgtcgaagat gcgcaggaag acgaggccgc tgctcttctc
    54481 gagcaggctc gcggtcggcg cctgggacga cgaggtccag tcgcggaaca gctcgatgat
    54541 ctcggcctcg aaggtcgaga ggtcgatcgg ggggagcggc tcgagcgtgt ccagcaggtg
    54601 ctggtaggcc tcgtagacgt tcccctcggc gaccgcgtgg cgcatcccga actgcgcccg
    54661 ccagagccgc tcgtcgagcc agccgagcat gccgaagtcg atccacccga gcgtgccgcc
    54721 gggcatcacg atcaggttgg ccgcgtgggg gtcggcgtgg aagatccggt ggcgcatgct
    54781 ctgctcgagg atcgagcgca tcaccagctc ggccgtgcgc tcgggggaga tccccatctc
    54841 ggcgaagcgg gccagcgcgt cccggtcgcc ggtctcgagc gcggcgatta gcgcggcgac
    54901 cgacaccccc tcgagccgct ccatcgtgag cacgcgccgg ccgcacagct cgggcacgag
    54961 cgcggggctg tagtggtcca cgccgtcggc gagcatcgcc tcgtgcatct cccacacgtt
    55021 gcgggcctcg cggcggaagt cgagctcctc gcgcgtgagg cggatcagct cgcgcacgag
    55081 ccgcctcagg tcgagctcgc cgaacgcgcc gagcccgccg agcagccgcg cgccgagccg
    55141 catgtacacg aagtccacgc ggaatcgctg cggcgtgccg gggcggatga ccttgaccac
    55201 gaccgactgg ccgttgtgca ggcgcgccgc gtggacctgc gctatcgacg ccgccgcgag
    55261 cgcctcgggc tcgagctcgc cgaagcagtc ctcgatcggc acctggaggt cgtcctcgat
    55321 cacctgcacg accgtctcga aggggacggg cggcaggcga tcgagcagct tggagagctc
    55381 ctcgcagtag cgcgcgggga gcaggtcgta gcgcatcgcg agtatctgcc ccagcttcac
    55441 gaacgcggcg ccggtcgact ggaggtacca gcgcacgagc cgcgggccgg agctcgggct
    55501 caccagccgg tggagggcga aggcgaccgc gaacgcggtg ctgaacgcga cgatccggat
    55561 cgagcgcgcg acgaacagca gcgggagcgg gagctgccgc gggcggcggc gggtagcgcg
    55621 cgggcgcgga cccgggtcct cggcgaaccc gtcgaggtcg gggacgccgg cgggcgcggc
    55681 gagttgcggc tcgtcccggg cgggtggtgt cctgaggtcg gtcaacttct ccacgcggac
    55741 gcccgacttc ggtcgcagcc cgcgcgcgta acctagcccg ccggggccgc gcgcgcagtt
    55801 gttgcgagcg gccgcccagc cgctaggcgt gctgcacggc gcccgccgtg ctcgcgacca
    55861 gcgcccgcgc gtgcgcctcg accgcgtccg cgagatcgcg gaggccgggc cgcgcgccgg
    55921 cggggagagc ggcccgcgcg cgctcggcgt agagcgcgac ctggtcggcc gcccgctcga
    55981 tcgccccggt cgcgctcgct cgcgcgatca cctcgtcgtg gcggtcgagt acgtcgtcgc
    56041 cgctcagcag cctcccgagc gcggcatcgt ccgccgcggc ccacagcacc ggcagcgggt
    56101 aggcgccgag cgacagctcg gttcccgccg ggcggttggt cggctcgtcg ccatcgagca
    56161 ggaccgcgag gtcctcggcc agccgcacgg cgacgcccag ttcgcgaccg taggcgacga
    56221 ggtcggcgat cgcggcggcg gagaacccgg cgctctcccc cgcgaagcgc agcgacagct
    56281 cgagcagcgt ggacatccgg gcgcgggccg tctccacgac ggcctcgggc gcgcgcgcga
    56341 gatcgaagag cgagcggctc tcgacgaact gcgcgtcgca gatcgcctgg atcgcgcggc
    56401 ccgtggccgc ggcgaagccc gacccggcgg cggccgcgtg ccggctcgcc cggctgagcg
    56461 cgaagtcgcc gaccagcagg cacatcgcgt tgttggcctg gccgccgcgc gtgcgcggcc
    56521 ggtcgttgac ctcgcgcgcg acgttggtcc cggctcgcac cagctcggcg gcggccgcgg
    56581 cgctcatcga ccgcgggtcg tcgtgccccc cgccgtcgcg ggtgagcgcg gcggctaccg
    56641 acaggacggg cccgaggccg cgccggacct cgctgtggag cagctccgcg gcgaccggga
    56701 acgacgtcgc caccgtgtcg agcacgaggt ggttcagcct ctcgagccac tcggcaccgg
    56761 gcccgccgcg gaggacgccg ccggcgaccg tctcggtcag gctcggctgg tcctcgggcg
    56821 aggtgacgac gttgcgcatc gtgcgaacga acggatgggc gctcgtcttc tggcggtagt
    56881 cgtccacctg gtggcggcgc cggtcggcca tgcgcagcgg cagaccgacg tcctgcccga
    56941 gtcgcgggaa ccgccgcgcc agcgcgcgcc cggcgccgag gccggcgcgc ccgttgcggg
    57001 accgctcgtg cacgagctcc gcgatcatct cggcgccgtg cagggcgtag gcgatgccct
    57061 cgccgctgac ggggtcggtg gtgcgggcgg cgtcgccgat cagcacgacc ccgggcgcct
    57121 cgcagcggcc cgggtcgaac tgcacgccca gcggcgcgcc aaacagcttg cttcgctcct
    57181 cgatgtcgcc gtaacgggac cgctcgcggg tgcggagctg gtccacgaac gcggccagca
    57241 cgtcgcggat gcgcttcggc gaggacacgc cgtcggcgcg ccagtagccg acgccgatgt
    57301 tcgcggtgtg gcggtcgatc gggaacaccc acccgtagcc cgggaacctc aggtcgccga
    57361 gctcgagcgg catgtaggcg ttgaagaccg gagcgacctc gtgctcgcag cggtagtagg
    57421 cgcgcaccgc gtacgcgccc gccgtctcgc tccgccgcga gcggccgatc tggccgatct
    57481 ggcgggcgag gcgggaggtg gacccgtccg cggccacgac aaaccgcgcg cggagcgcga
    57541 cgtcccgggg ctcggcccgg cgcgtgacca ggacggtctg ctcctccccc gcgaccatgc
    57601 tcgtggcccg cgcctccagg aagcgcgccc cgcgcgcccg cgcgaggtcg agcagcgccg
    57661 agtcgagcac ccggcgctcg acgcagtacg cctggtggcc accgggggag gggtcgtagc
    57721 gccggtactc cgacgagcgg tagccgaaca ccatccgcag gccctcgatc ggcggccgcc
    57781 ccccgaacag gtccggcagg ccgagccggt cgaggccggc cacggccgag cgcgtaagcc
    57841 cgtcgccgca cggcttgtcg cgcgggaagg ccgattggtc cacgagcagg acctcgtgcc
    57901 cgcgctccgc cagatgagcc gcgcaggacg atcccgccgg gccggcgccg accaccacga
    57961 tctccgcctc gtcgatcggc tcagccgcgt ggagcatcgg ccccgatcgc gtcgagcacg
    58021 ggcagcgccc cgtcgaggaa gtccacgagc gccggcgcgt cgatgctcgc cgcggcgcgc
    58081 gcggcgcggt c
//
LOCUS       DASVPL010000139         7927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_291537, whole genome shotgun sequence.
ACCESSION   DASVPL010000139 DASVPL010000000
VERSION     DASVPL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291537"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VF517_07290"
     CDS             <1..995
                     /locus_tag="VF517_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX8052780.1"
                     /translation="YNDTFGHPAGDALLARLGRAMSESVEGCGTAYRPGGDEFCALLQ
                     VDSRQLAGKIGDTVAALSERGKGFAVTPSYGSVVVPDEARDVTLALQLADKRLYEQKG
                     DRRRARDGTQVRDVLMQALRERRPDLDEHIGGVASLAHAVARRFELNTEQVDEVTKAA
                     ELHDIGKMAVPDAILEKPASLDEREREMIKQHTVVGERILAVAPALRKIGKLVRYSHE
                     RWDGTGYPDRLKGDEIPLGARIIAVCDAFDAMTTDRPYQPSVPLDEALDELRRCAGTQ
                     FDPSVVEVFCQEAEAVLRDLDAHDDEPDLVDAGLDALVLRVPEGEPAPAAPDGD"
     gene            complement(1000..1482)
                     /locus_tag="VF517_07295"
     CDS             complement(1000..1482)
                     /locus_tag="VF517_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052781.1"
                     /translation="MKRSILLILTAALLGLGLLAGCGGDDGGDDAAAQKEDFAREFKP
                     INADFLKLGQDVQQTIQTADTKSDEELATKFKSLSDQVLAVRTRLTGLEAPDEYKEDV
                     DRLAASMQVVAGDLGEISAAAEGSRAAEAKTQVQELVRHSVETRTARRALARKTGAQQ
                     "
     gene            1537..1989
                     /locus_tag="VF517_07300"
     CDS             1537..1989
                     /locus_tag="VF517_07300"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8052782.1"
                     /translation="MRRLEREQHLPGEPDEVFPFFADALNLERITPPWLGFRVTTPGT
                     IEMGPGTLIEYRLRLHRVPVRWLTRIEVWEPPERFVDTQVRGPYRVWRHEHRFAPAPG
                     GGALMRDRVDYELPLGPLGDLAHALIVRRDLARIFDHRHSAVAAYFTK"
     gene            2002..3765
                     /locus_tag="VF517_07305"
     CDS             2002..3765
                     /locus_tag="VF517_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8052783.1"
                     /translation="MKTFVRLLGFLRPYRRAVIMSFVLAAVAMVAGVLIPFLVGETVD
                     DIRRGETHLWPLAGAIVAAGLLRLAFSVARRLVAGRVSLGVEYDLRNRVYSHLQSLEV
                     AFFDEQQTGQLMSRSTVDLQAVRFFLGYGLIFIAQSAVTIVVAAIVMLAINASLAWIA
                     LVPAPFVFFAAYRYGARNRPASQEVQQRIAELTAEAEESISGIRLVKAFAREERQLAR
                     FTRATARVFEQSMVSTRLRAFYNPFIGFLPQVGLAAVLLVGGHQAIDGRITVGEFVAF
                     YGYVLMLIGPVRMLGIALGMAQRAVASGARVFELLDREPRMTSPPDAQPLPDGDGRVE
                     IRGVSFSYETGPPVLRDVDLDVPAGRTIAIVGPTGSGKTTLVSLLPRLYDPLEGSVTV
                     DGVDLRDVDLESLRSEVGLVSDEPFLFSATLRENIAYARPDATDDEVEQAARRAGIHD
                     YVTGLPDGYATRVGERGLTLSGGQRQRVAIARAIIKDPRILILDDATSSVDATTEAQI
                     KSALRELMRDRTTFVIAHRLSTIALADEVVVLEGGTIAARGTHDELVESSPLYAEIAE
                     RGLPDKVFLTRKPVEQELAGL"
     gene            3762..5546
                     /locus_tag="VF517_07310"
     CDS             3762..5546
                     /locus_tag="VF517_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8052784.1"
                     /translation="MSDRLRDRGPTRDVRRQRLAGLILLLRPYRGRVALMFVAMVLAI
                     GAALVPPYLAGRAIDDGIRAGDVDFLELIVGLFLAAAVINWVATYAQTYLVNWVGQRA
                     LQDLRLQIFRHLERMSIGFYARRKTGVLISRITNDVQALDQLVTDGMVTLFSSTLTLL
                     GITAILVFLDPGLALVTFAVFPVLAAASIAFRIASMGAYRLTRETVAYVTAYLQETLS
                     GVRVVRTFGQEARHERRFAELNEENRAANMKTVYLNAAYFPSVELISAVATAAILLYG
                     GHQVVEGAVTIGVLASFVFYLQSFFDPIQQLSQLYTTYQSGMAALDKIFELLDEEPEL
                     EDGPDAVELPQVRGEIRFEDVTFQYDPDDEATRALDGVDLTVAPGQTVALVGATGAGK
                     STLAKLVARFHDPTSGRVLVDGHDLRDVTERSLRSQLGIVPQEGFLFSGTIADNIAFG
                     RPDATREEVEEAARAVRAHEFVERLEDGYDTEVGERGGHLSAGQRQLVAFARAAIADP
                     RILILDEATSNVDVRTEARIEQGLRRLLAGRTAIVIAHRLSTIRGAARIVVLEHGAVV
                     EQGTHDELVEAGGHYARLVRDWAEQTAA"
     gene            complement(5543..5977)
                     /locus_tag="VF517_07315"
     CDS             complement(5543..5977)
                     /locus_tag="VF517_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX8052785.1"
                     /translation="MRKGAAALAATLALAGCGEDRSASVSTQTDPPPPRPAAAAPAAP
                     ATPPPAQVTLAESEYKLEPARIRVDRPATLAIEVRNTGAERHALVVAGIPAPARTRVL
                     GPGSDQVLTVELKKPGRYRWFCPVDGHARKGMRGTIQVARGD"
     gene            complement(5974..6444)
                     /locus_tag="VF517_07320"
     CDS             complement(5974..6444)
                     /locus_tag="VF517_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX8052786.1"
                     /translation="MNRKLLAMALMGAALALGACGDDDDGGSDAGDTGTPEQTSTQAA
                     PAPEQDTEGGQSGTQKGGGTQLTNDADASGQLKFEKSSLEAPAGKVTITMTNPSDLPH
                     AIEVEGNGVEEEGETVQKGGKSVVTAELKPGEYEFYCPVPGHKEGGMTGTLTVK"
     gene            complement(6528..7133)
                     /locus_tag="VF517_07325"
     CDS             complement(6528..7133)
                     /locus_tag="VF517_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052787.1"
                     /translation="MSRWRATPRQLAILLSGLWLFGIGEALLIESRLGNSPWTVFAEG
                     AGEQTGIGVGAATVATSFLILAAWIPLRQQPGLGTIANALVIGISIGVTIEVVPDLDS
                     LGPRVGQVVVGIGIVAIGSGLYLMTRLGAGPRDGLMLGLHRRTGLSVRLIRTGIEGSA
                     TAAGALLGGKVGLGTLAFAVLIGPGVQAALGVLSSTPAHEL"
     gene            complement(7254..>7927)
                     /locus_tag="VF517_07330"
     CDS             complement(7254..>7927)
                     /locus_tag="VF517_07330"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX8052788.1"
                     /translation="PHEEDAVRAHVAGCPDCARDHAELAPVPALLGAVEDPEAVPAEP
                     PPTLEDAVLDRFVREGARPEAGARRRRAFRWLARPLPAAALAATAAVVLTLAATGAFD
                     RDGEGPKVYGAHLRAAAGGAPAGGGAERPYAYVHLSSLDTGTRVELKASGLAGAPGAV
                     YELWCIYPDGSRVSGGTFRADPAGRATATMTTAARVGEYHRLAVERHAPGEPGQRVLA
                     GDIAY"
BASE COUNT         1025 a         2924 c         2845 g         1133 t
ORIGIN      
        1 gctacaacga caccttcggc caccccgccg gcgacgcgct gctcgcgcgc ctcggccgcg
       61 ccatgtccga gtccgtcgag ggctgcggca cggcctaccg ccccggcggc gacgagttct
      121 gcgcgctgct ccaggtcgac agccgccagc tcgccggcaa gatcggggac acggtcgcgg
      181 cgctctcgga gcgcggcaag ggcttcgccg tgaccccctc ctacggctct gttgtggtcc
      241 ccgacgaggc gcgcgacgtg accctcgcgc tccagctcgc ggacaagcgc ctgtacgagc
      301 agaagggcga ccgccgccgt gcccgcgacg gcacgcaggt acgcgacgtg ctgatgcagg
      361 cgctgcgcga gcgccggccc gacctcgacg agcacatcgg cggcgtcgcg tcgctcgcgc
      421 acgccgtggc ccgtcgcttc gagctcaaca ccgagcaggt cgacgaggtc accaaggccg
      481 ccgagctgca cgacatcggc aagatggccg tgccggacgc gatcctcgag aagcccgcct
      541 cactggacga gcgcgagcgc gagatgatca agcagcacac ggtcgtcggc gagcggatcc
      601 tggccgtcgc ccccgcactg cgcaagatcg gcaagctcgt gcgctactcg cacgagcgct
      661 gggacggcac cggctacccc gaccgtctca agggcgacga gatcccgctc ggcgcgcgga
      721 tcatcgccgt ctgcgacgcg ttcgacgcga tgacgaccga ccggccctac cagccgtccg
      781 tgccgctcga cgaggcgctc gacgagctgc gtcgctgcgc cggcacccag ttcgacccga
      841 gcgtggtcga ggtcttctgc caggaggccg aggccgtcct gcgcgacctc gacgcgcacg
      901 acgacgagcc cgacctggtc gacgccggcc tggacgcgct cgtcctgcgg gtgcccgagg
      961 gcgagcccgc ccccgcggcg cccgacggcg actgacgagc tactgctgag cgccggtctt
     1021 gcgggcgagc gcgcgccgcg ccgtgcgcgt ctcgacggag tggcggacca gctcctggac
     1081 ctgcgtcttg gcctccgccg cgcggctgcc ctcggccgcc gccgagatct cgcccagatc
     1141 gccggccacg acctgcatcg aggccgccag ccggtcgacg tcctccttgt attcgtcggg
     1201 cgcttcgagg ccggtgagac gcgtcctcac cgcgagcacc tggtcggaga gcgacttgaa
     1261 cttcgtggca agctcctcgt ccgacttggt gtcggcggtc tggatcgtct gctgcacgtc
     1321 ctggccgagc ttgaggaagt cggcgttgat cggcttgaac tccctggcga agtcctcctt
     1381 ctgcgccgcg gcgtcgtcgc cgccgtcgtc gccaccgcag ccggccagca ggccgagccc
     1441 aagcagggcc gcagtgagga tcagaaggat cgagcgcttc atcggcggcg atcctagcga
     1501 ccgatccggt cggcaattgc ggccgtaccc agggagatga ggagactcga gcgcgagcag
     1561 cacctgcccg gcgagccgga cgaggtgttc ccattcttcg ccgacgcgct caacctcgag
     1621 cggatcacgc cgccgtggct gggcttccgg gtgacgacgc ccggcacgat cgagatgggc
     1681 cccggcacgc tgatcgagta ccggctgcgc ctgcaccgcg tgccggtgcg ctggctgacc
     1741 cggatcgagg tctgggagcc gcccgagcgg ttcgtggaca cgcaggtgcg cgggccatac
     1801 cgagtgtggc gccacgagca ccgtttcgcc cccgcgcccg gcggcggagc cctcatgcgc
     1861 gaccgcgtgg actacgagct tccgcttggc ccgctcggcg atctcgcgca cgcgctgatc
     1921 gtgcgccgcg acctcgcgcg gatcttcgat caccgccact cggccgtggc ggcgtacttc
     1981 actaagtaaa gtaagggtca cgtgaagacc ttcgtacgcc tgctcggctt tctccgcccc
     2041 taccgccgcg cggtgatcat gtcgttcgta ctggccgcgg tggcgatggt cgcgggcgtc
     2101 ctgatcccgt tcctggtcgg cgagacagtc gacgacatcc gccgcggcga gacccacctc
     2161 tggccgctgg cgggcgcgat cgtggccgcg ggcctcctgc gcctcgcctt cagcgtcgcg
     2221 cggcgcctgg tggccgggcg cgtctcgctc ggggtcgagt acgacctgcg caaccgcgtc
     2281 tactcgcacc tccagtcgct cgaggtcgcg ttcttcgacg agcagcagac cggccagctc
     2341 atgtcgcgct cgaccgtgga cctgcaagcc gtgcgcttct tcctcggcta cggcctgatc
     2401 ttcatcgcgc aatcggcggt cacaatcgtg gtcgcggcga tcgtgatgct ggcgatcaac
     2461 gcgagcctcg cgtggatcgc gctcgttccg gcgccgttcg tcttcttcgc cgcctaccgc
     2521 tacggagcgc gcaaccgccc ggccagccag gaggtccagc agcggatcgc cgagctgacc
     2581 gcggaggctg aggagagcat ctcgggcatc aggctcgtta aggcgttcgc gcgcgaggag
     2641 cgccagctcg cgcgcttcac ccgcgcgacg gcacgcgtct tcgagcagtc gatggtctcg
     2701 acccggctgc gcgccttcta caacccgttc atcggcttcc tgccccaggt cggcctcgcg
     2761 gccgtgctgc tcgtcggcgg gcaccaggcg atcgacggcc gcatcacggt cggcgagttc
     2821 gtcgccttct acggctacgt gctgatgctg atcgggccgg tgcggatgct ggggatcgcg
     2881 ctcgggatgg cccagcgcgc ggtcgcctcc ggcgcgcgcg tgttcgagct gctcgaccgc
     2941 gagccgcgca tgacctcgcc gcccgacgcg cagcccctgc cggacgggga cggccgggtg
     3001 gagatacgcg gcgtgtcgtt ctcgtacgag acgggtccgc cggtgctgcg cgacgtcgac
     3061 ctcgacgtgc cagccggccg caccatcgcg atcgtcgggc ccaccggctc gggcaagacc
     3121 acgctcgtct cgcttctgcc gcggctctac gacccgctcg agggcagcgt gacggtggac
     3181 ggagtggacc tgcgcgacgt ggacctcgag tcgctgcgca gcgaggtcgg cctcgtctcc
     3241 gacgagccgt tcctcttctc ggcgaccctc cgcgagaaca tcgcctacgc gcggccggac
     3301 gcgaccgacg acgaggtcga gcaggcggcg cggcgggcgg gcatccacga ctacgtgacc
     3361 gggctcccgg acggctacgc gacgcgcgtg ggcgagcgcg ggctcacgct ctcgggcggc
     3421 cagcgccagc gcgttgcgat cgcgcgggcg atcatcaagg acccgcgcat cctgatcctc
     3481 gacgacgcca cctcgagcgt cgacgccacc accgaggcgc agatcaagag cgccctgcgc
     3541 gagctgatgc gcgaccggac caccttcgtc atcgcgcacc gcctctcgac gatcgcgctg
     3601 gccgacgagg tggtcgtgct cgagggaggc acgatcgccg cgcgcggcac gcacgacgag
     3661 ctggtcgaga gctcacccct ctacgcggag atcgccgagc ggggcctgcc ggacaaggtc
     3721 ttcctcaccc gcaagccggt cgagcaggag ctggccggac tgtgagcgac cgcctccgcg
     3781 accgcggccc cacgcgcgac gtccgccgcc agcggctcgc cgggctgatc ctgctgctgc
     3841 gcccctaccg agggcgcgtg gcgctgatgt tcgtggcgat ggtgctcgcc atcggcgccg
     3901 cgctcgtgcc gccctacctg gccggccgcg cgatcgacga cggcatccgc gccggcgacg
     3961 tcgacttcct cgagctgatc gtggggctgt tcctcgcggc ggcggtgatc aactgggtcg
     4021 ccacctacgc gcagacctac ctcgtcaact gggtcggcca gcgcgcgctc caggaccttc
     4081 gcctccagat cttccgccac ctcgagcgga tgtcgatcgg cttctacgcg cgccgcaaga
     4141 cgggcgtcct gatctcgcgg atcacgaacg acgtgcaggc gctcgaccag ctcgtgaccg
     4201 acggcatggt cacgctgttc tcctccacgc tcacgctgct cggcatcacg gcgatcctgg
     4261 tcttcctcga cccgggcctc gcgctcgtca cgttcgcggt cttcccggtg ctggcggcgg
     4321 cgagcatcgc gttccggatc gcgtcgatgg gcgcctaccg gctcacgcgc gagaccgtcg
     4381 cgtacgtgac cgcgtatctc caggagacgc tgtcgggcgt gcgcgtggtg cgcaccttcg
     4441 ggcaggaggc gcgccacgag cgccgcttcg ccgagctgaa cgaggagaac cgcgcggcga
     4501 acatgaaaac ggtctacctg aacgcggcgt acttcccgtc ggtcgagctg atctcggccg
     4561 tcgcgaccgc tgcgatcctg ctctacggcg gccaccaggt ggtcgagggc gcggtcacga
     4621 tcggcgtgct cgcctcgttc gtgttctacc tccagagctt cttcgacccg atccagcagc
     4681 tctcgcagct ctacacgacc taccagtccg ggatggcggc gctcgacaag atcttcgagc
     4741 tgctcgacga ggagccggag ctggaggacg gacccgacgc ggtcgagctg ccgcaggtgc
     4801 gcggcgagat ccgcttcgag gacgtgacgt tccagtacga ccccgacgac gaggccaccc
     4861 gcgcgctcga cggcgtggac ctcaccgtgg cgccggggca gacggtcgcg ctggtcggtg
     4921 ccaccggcgc cggcaagtcc acgctcgcga agctggtcgc gcggttccac gaccctacgt
     4981 cggggcgcgt gctggtcgac gggcacgacc tgcgcgacgt gaccgagcgc tcgctgcgct
     5041 cccagctcgg gatcgtgccg caggagggtt tcctgttctc ggggacgatc gccgacaaca
     5101 tcgccttcgg gcgccccgac gcgacgcgcg aggaggtcga ggaggcggcg cgcgccgtgc
     5161 gtgcgcacga gttcgtggag cggctcgagg acggctacga caccgaggtc ggcgagcgcg
     5221 gcgggcacct ctcggccggg cagcgccagc tcgtcgcgtt cgcgcgcgcg gcgatcgcgg
     5281 acccgcggat cctgatactc gacgaggcca cctcgaacgt ggacgtgcgc accgaggcgc
     5341 ggatcgagca gggcctgcgg cgcctgctcg ccggccgcac ggcgatcgtc atagcccacc
     5401 ggctctcgac gatccgcggc gccgctcgga tcgtggtgct ggagcacggc gcggtggtcg
     5461 agcagggcac gcacgacgag ctggtcgagg ccggcggcca ttacgcgcgg ctcgtgcgcg
     5521 actgggccga gcagaccgcg gcctagtcac cgcgcgccac ctggatcgtc ccgcgcatgc
     5581 ccttcctggc atgcccgtcg accgggcaga accagcggta gcggccgggc ttcttcagct
     5641 cgacggtcag cacctggtcc gacccgggcc ccagcacccg cgtgcgggcg ggcgctggga
     5701 tgcccgccac caccagcgcg tggcgctcgg cgcccgtgtt gcgaacctcg atcgccagcg
     5761 tcgcgggacg gtcgacgcgg atccgcgccg gctcgagctt gtactcgctc tcggccagcg
     5821 tgacctgggc cggtggcggg gtcgcgggtg ccgccggcgc cgcggcggcg ggccgcggcg
     5881 gcggcggatc ggtctgcgtc gaaaccgagg cgctgcggtc ctcgccgcac ccggccaggg
     5941 ccagggtggc ggcgagggcc gcggccccct tcctcacttg acggtgagcg tccccgtcat
     6001 gccgccctcc ttgtgccccg gcaccgggca gtagaactcg tactcgcccg gcttgagctc
     6061 ggccgtgacc acggacttgc cgcccttctg gacggtctcg ccctcctcct ccacgccgtt
     6121 gccctcgacc tcgatcgcgt gcggcaggtc ggacgggttg gtcatcgtga tcgtgacctt
     6181 gccggccggg gcctcgagcg aggacttctc gaacttgagc tggccggacg cgtccgcgtc
     6241 gttggtgagc tgcgtgcctc cgcccttctg ggtaccggac tggccgccct cggtgtcctg
     6301 ctcgggggcg ggggccgcct gggtcgaggt ctgctccggg gtgccggtgt cgccggcgtc
     6361 cgagccgccg tcgtcgtcgt caccgcacgc gccgagggct agagcggcgc ccatcagggc
     6421 catggcgaga agcttgcggt tcatgtctgt gcctccggag ggtggggaaa acgtgttgga
     6481 cgctaccgca gggtcctacg cacgtggggg ccggggggtt cggtggctca gagctcgtgg
     6541 gccggcgtgg agctgagtac gccgagcgcc gcctgcacgc ccggacctat caggactgcg
     6601 aacgcgagag tgccgaggcc gaccttgccg ccgagcagag cgccggcggc ggtcgcgctg
     6661 ccctcgatcc cagtgcggat caggcgcacc gagagccccg tgcggcgatg caggccgagc
     6721 atcaggccgt cgcgcgggcc ggcgccgagg cgtgtcatca ggtagaggcc gctgccgatc
     6781 gcgacgatcc cgatgcccac cacgacctgg cccaccctgg ggccgaggga gtcgaggtcc
     6841 ggcacgacct cgatcgtcac gccgatcgat atcccgatca cgagcgcgtt ggcgatcgtc
     6901 ccgaggcccg gctgctggcg cagcggtatc cacgcggcga ggatcaggaa gctggtcgcg
     6961 accgtggcgg cgccgactcc gatgccggtc tgctcgccgg ccccctccgc gaacaccgtc
     7021 cagggcgagt tgccgagccg cgactcgatc agcagggcct cgccgatccc gaacagccag
     7081 aggccgctca gcaggatcgc gagctgtcgg ggggtggcgc gccagcggct cacgcgctcg
     7141 aggaaagcag gcggctccgg cgggcggcgg gcggcggctc cgcggctcgg gcgcgtgggc
     7201 ggcggccccg cagctcgggc gcggcggcgg ccccgcggcg cggtgccgcg gggctagtac
     7261 gcgatgtcgc ccgcgagcac gcgctggccc ggctcgcccg gagcgtgccg ctcgacggcc
     7321 aggcggtggt actcccccac ccgcgcggcc gtggtcatcg tggccgtcgc ccggcccgcg
     7381 gggtccgcac ggaaggtccc accgctgacc ctcgagccgt ccgggtagat gcaccacaac
     7441 tcgtagaccg cgcccggcgc gcccgcgagg ccgctcgcct tcagctcgac gcgcgtgcct
     7501 gtgtcgaggc tcgagaggtg gacgtaggcg tacggccgct cggcgccgcc cccggccggg
     7561 gcgccgccgg ccgccgcgcg caggtgcgcg ccgtagacct tggggccctc gccgtcgcga
     7621 tcgaacgcgc cggtggccgc gagggtcagc acgaccgcgg ccgtggctgc cagcgccgcg
     7681 gccgggagtg gccgcgcgag ccagcggaac gcgcgacgcc ggcgcgcgcc cgcctccggg
     7741 cgcgcgccct cgcgcacgaa gcggtcgagc acggcgtcct cgagcgtcgg aggcggctcg
     7801 gccgggaccg cctccggatc ctccacggcg ccgagcaggg ccgggaccgg ggccagctcg
     7861 gcgtggtcgc gcgcgcagtc ggggcagccg gcgacgtgcg cgcgtacggc gtcctcctcg
     7921 tgcggct
//
LOCUS       DASVPL010000140         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_371744, whole genome shotgun sequence.
ACCESSION   DASVPL010000140 DASVPL010000000
VERSION     DASVPL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371744"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VF517_07335"
     CDS             <1..1355
                     /locus_tag="VF517_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052789.1"
                     /translation="GRGGEVREGGTRVGFVEKVTSVKLPNGEAGAEFDLKLDKSVGDV
                     PEDTTFTIRPRSPLGLKYLEMDRGESDTAVRQGHTFPAAQTTLPVEIDDFNRIYDERT
                     RQGVRRNIDEFGNTFAGRGVALNETIGELPRLFGLIHPVTRNLADPRTQIGRFLRELA
                     DAARVTEPIADVQADLFTRGAQTFEAVSRDTGALQETIRRSHELFQTGIESFPVQRPF
                     LTESARLARAMRPVARELRPTLPLINPALARGIPVTRRSVEFYEDLKPVLVSLRDLAT
                     DPASGIALRALGGTSRTLKPQIEFLGPFQTVCNNWNYWWTFLAEHLAAESGQGFSQRT
                     ESKSAAPQTNNQGSMGAYEPVNGQGYNQGMARRGSNMHLHSTPYNHAITDTGEADCEN
                     GQRGYLRRYTAVKEPEFLIQTLSDIPGVQGPTYRGRPRVPKGQTFTREPGGKGHVHSP
                     "
     gene            1352..>2089
                     /locus_tag="VF517_07340"
     CDS             1352..>2089
                     /locus_tag="VF517_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8052790.1"
                     /translation="MRRQRGMPPFRVGLISILLVVVAVYFAFARGVPWGDHYELKVHF
                     EDSVNVKERQPVRIAGVDVGQVVKVEHADPPGRLVEVTVRIEDGGRPIHRDAQVKIRP
                     RMFLEGNWFLDMSPGSDASPELEDGGTIPVTQTSSPVQLGQVFAILESDTRENIRKIF
                     REYGSALEGEGAGGFNRSIKYWEPAYRNGAIVQDATLGLNPHDLSDYIDSAGRVARGL
                     DRSPDALRSLITDFDTAAGAFAREATSL"
BASE COUNT          333 a          751 c          733 g          272 t
ORIGIN      
        1 tcgggcgcgg cggcgaggtg cgcgagggcg gcacccgcgt cggcttcgtc gagaaggtca
       61 cctcggtgaa gctgccgaac ggcgaggccg gggccgagtt cgacctgaag ctcgacaagt
      121 cggtcggcga cgttccggag gacacgacgt tcacgatccg gccgcgctcg ccgctggggc
      181 tcaagtacct cgagatggac cgcggcgagt ccgacaccgc cgtccggcag ggccacacgt
      241 tccccgcggc gcagaccacg ctgccggtcg agatcgacga cttcaaccgg atctacgacg
      301 agcgcacccg ccagggcgtg cgccgcaaca tcgacgagtt cggcaacacg ttcgccggcc
      361 gcggcgtcgc gctcaacgag acgatcggcg agctgccgcg gctgttcggc ctgatccacc
      421 cggtcacgcg caacctcgcc gatccgcgga cgcagatcgg ccgcttcctg cgcgagctgg
      481 ccgatgcggc gcgcgtgacc gagccgatcg ccgacgttca ggccgacctg ttcacccgcg
      541 gggcgcagac gtttgaggca gtctcgcgcg acaccggcgc gctccaggag acgatcaggc
      601 gctcgcacga gctgttccag accgggatcg agtcgttccc cgtccagcgc ccgttcctta
      661 ccgagagcgc gcggctcgcc cgcgcgatgc ggccggtcgc ccgtgagctc cggccgacgc
      721 tgccgctcat caacccggcg ctcgcgcgcg gcatcccggt cacccggcgc tcggtcgagt
      781 tctacgagga cctgaagccg gtcctcgtct cgctgcgcga cctcgcgacc gaccccgcca
      841 gcgggatcgc gctgcgcgcc ctcggcggca cctcgcgcac gctcaagccg cagatcgagt
      901 tcctcgggcc gttccagacg gtctgcaaca actggaacta ctggtggacg ttcctggcgg
      961 agcacctcgc cgccgagtcc ggccagggct tcagtcagcg cacggagtcg aagagcgcgg
     1021 cgccgcagac gaacaaccag gggtcgatgg gcgcctacga gccggtcaac ggccagggct
     1081 acaaccaggg gatggcccgg cgcgggtcga acatgcacct gcactcgacg ccgtacaacc
     1141 acgcgatcac ggacaccggc gaggccgact gcgagaacgg acagcgcggc tacctgcgcc
     1201 gctacaccgc cgtcaaggag ccggagttcc tgatccagac gctctccgac atcccgggcg
     1261 tgcagggccc gacctaccgc ggccggccgc gcgtgccgaa ggggcagacc ttcacccgcg
     1321 agccgggcgg gaaggggcac gtccactccc catgaggcgc cagcgcggca tgcccccgtt
     1381 ccgggtcggg ctgatctcga tcctcctggt cgtggtcgct gtgtacttcg cgttcgcgcg
     1441 cggggtgccg tggggcgacc actacgagct gaaggtccac ttcgaggact cggtgaacgt
     1501 gaaggagcgc cagccggtcc ggatcgcggg cgtggacgtg ggccaggtag tgaaggtcga
     1561 gcacgccgac ccgcccggca ggctcgtcga ggtgacggtg cgtatcgagg acggcggtcg
     1621 cccgatccac agggacgcgc aggtgaagat ccggccgcgc atgttcctcg agggcaactg
     1681 gttcctcgac atgagccccg gcagcgacgc gagcccggag ctcgaggacg gcggcacgat
     1741 cccggtcacg cagacctcgt cgccggtcca gctcgggcag gtgttcgcga tcctcgagag
     1801 cgacacgcgc gagaacatcc gcaagatctt ccgcgagtac ggctcggcgc tggagggcga
     1861 gggagccggc ggcttcaacc gctcgatcaa gtactgggag ccggcctacc gcaacggggc
     1921 gatcgtgcag gacgcgacgc tcgggctgaa cccgcacgac ctgtccgact acatcgacag
     1981 cgccggccgg gtggcgcgcg ggctcgatcg cagcccggac gcgctgcggt cgctcatcac
     2041 cgacttcgac acggccgccg gggcgttcgc gcgcgaggcc acgagcctc
//
LOCUS       DASVPL010000141         9919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_291605, whole genome shotgun sequence.
ACCESSION   DASVPL010000141 DASVPL010000000
VERSION     DASVPL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291605"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF517_07345"
     CDS             complement(<1..376)
                     /locus_tag="VF517_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX8052791.1"
                     /translation="MSDSEGRRGRFTPLKRLSDDELAAEAARGDDKAFALLFERLQDP
                     LYRYTLSILGNPEEARDALQNAMLKAYRALRGETREIAARPWLYRIARNEAVTALRGR
                     RPLGELEEAVGVAAAAGAGADAD"
     gene            complement(519..1100)
                     /gene="clpP"
                     /locus_tag="VF517_07350"
     CDS             complement(519..1100)
                     /gene="clpP"
                     /locus_tag="VF517_07350"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX8052792.1"
                     /translation="MPLVPMVVEQTSRGERSFDIYSRLLNERIVFLGDQVDDQIANLI
                     VAQLLHLESDDPDKDVSLYINSPGGSVYAGLAIYDTMQFIKPDVVTICYGMAMSMGSL
                     LLTGGTPGKRFAHPNSRILIHQPSGGYQGQSADIEIHAREILDLRRRLDEIYAKHTTQ
                     DIEKVHVDMDRDRYFTPEEALEYGLIDRVISQH"
     gene            1179..2027
                     /locus_tag="VF517_07355"
     CDS             1179..2027
                     /locus_tag="VF517_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8052793.1"
                     /translation="MVEAAGTFELGGELEVNRLGFGAMRITGEGVWGDPPDPYAAMSV
                     LSHAVELGVNLIDTADSYGPEVSENLIAQTLHPYDGITIATKGGLTRPGPGVWERNGR
                     PEHLRSACEASLRRLRVDRIDLYQLHSPDRDVPYEESVGALGELQQEGKINLIGLSNV
                     SVEQLDAAMGIVDVVSVQNRYNLTDRGNEDVLDACEARGIGFVPWFPLATGSLAEQGG
                     VLDEAADRHDATPSQIALAWLLARSPVMLPIPGTGSVEHLEENCGAARIELDEDELEQ
                     IASAAG"
     gene            2024..3241
                     /locus_tag="VF517_07360"
     CDS             2024..3241
                     /locus_tag="VF517_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8052794.1"
                     /translation="MTAATELDLPSLEYQDAELRGDAFHDAMRELREQNWLARVDPVG
                     FAVLDREAAAHFMRSRSLTFPGRRMLEVQGVTEGPLYERFKGNLLDLDGDNHRRLRKL
                     VQPAFTPPAADRHRPVMRQLVEALHGSVAEAGRCDFVQSFAQPYPAMMIAHVMGAPLE
                     DAGRLHEWANYIQQQFDPIKLSIQLAEIERAAEEFQHYAWDLLRKRQGDPKDDLISEL
                     ITAEEEGDRLSEEECVHLVSAVLVGGVDTTQSQLAHGMLLFARHPDQWRMLAEDPSLA
                     PRAVEEVLRLDPITPFTARIALEDVEYRGVTFPKDTIVIAAAVTANRDPDAYEEPEAF
                     DITADRDRSKPLTFGAGPHFCLGASLARAELQEAFALLAPRMPDLELDGEPIFDTPFG
                     VYGMESLPIRWRA"
     gene            3368..4018
                     /locus_tag="VF517_07365"
     CDS             3368..4018
                     /locus_tag="VF517_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX8052795.1"
                     /translation="MRNYLRLGGVLAVLALAFAPGAQAGYRTQLERAGLTDLRTHAPE
                     LAVDMRYATDRNFTGAPLPGYCAQEPYLRLRAARALGRAQRRLRSDGLGLMVFDAYRP
                     ARASRAMVRWARRTGREDLLNGWIAARSNHNRGAAVDLTLVHLDTVKPLAMGTEYDAF
                     SSRSATAAVGGKALRNRLRLKRALEVEGFRNYRREWWHYDFPAELRAPRIDVALGC"
     gene            complement(4025..5920)
                     /locus_tag="VF517_07370"
     CDS             complement(4025..5920)
                     /locus_tag="VF517_07370"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEX8052796.1"
                     /translation="MTVRRAAVLALAVALCAPATGSAATPKSWPRAFRSCDALLTHAR
                     TQAARIAEPAGLPRRPQPGIAFPASGPREDSGAPQAAPADGAAAEAPEHSTTNVQEGG
                     VDEPDLVKTDGRHVFALASGTLRVVDVRGAEPKVVGSLKLGGFSQEMLLAGDRLMVFS
                     TPGYGDAQPLAADLIAQDPRAVLTEIDVSDRSNPRVVRTLDVDGSYVSARMRGTTARV
                     VIASYPRPIPVADGDAPEVQRRRAAIRRTHLKTWIPRYRLTSARGGNSVTRSLVGCRA
                     TRRPAVFSGLGMLTVLTIDLSKGIVPVDSDGLMTDARTVYASQGSLYVASERWLDPTP
                     ESGESVPGGRATTIHRFDARDSDHTTFRASGTVRGYLLNQWALSEYRDHLRVATTEEP
                     LWWGGGAPQPASESFVTTLAEDGGELRTRGQVGGLGRGERIYSVRFIEDQGYVVTFRQ
                     VDPLYVVDLSNPAKPAVAGELKVLGYSAYLHPVGRDLLLGVGQDASEEGRTQGTQVSL
                     FDVSDPRQPRRTHRRTLAGAGSEAEYDHHAFLWWPGRSLAVIPIRSYPRDGGEPFVGA
                     VGFGVAPATGIDEVGRIAHPQGSGGYVPDIRRALVVGDRLFTVSDAGIKASRLDTLGD
                     VAFAPFG"
     gene            complement(5917..8418)
                     /locus_tag="VF517_07375"
     CDS             complement(5917..8418)
                     /locus_tag="VF517_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052797.1"
                     /translation="MRGLRTIVVLALTALVLACAAASAAAAPAPAQNADDRYALANGC
                     FVLRSQSLGKFVGKAAGGGYSASAADVGGGEPLRMQATDLGKYLLYGKDQDFVAAGSG
                     DWAETATAPSEAAEWEVTVEGGAFKVVSLSTGKPLSVSPAGEVVLGGSGNAALFAFEI
                     AEGCATYPEAQVNATGKPYTGATPYGEARGFVDAHLHMMAFEFIGGSIRCGRPWHRYG
                     VPFAMVDCPDHGPGGYGAAAEGVLSYGNPVAPHDTVGWPTFKDWPAYRSLTHEQVYYK
                     GLERAWRSGLRVFVNLMVDNAQLCEVYPLKRNPCNEMNTVRIEIQRLRELERYIDAQN
                     GGPGKGWFRIVKDPFQARRTINEGKLAVVMGMEVSKPFDCDVQNDQPACDRGVITDWL
                     NELESAGLRQFELVNKFDNALVGVAGDSGSTGLVTTVGNRGETGNFFDYDDCKDENSD
                     RVPATQTPDHNTDRLFEIYAQYAPGGLLPLYGEGPQCNKRGLTDLGEFTIRELVKRKM
                     LFDPDHMSVLGRRQSLALTESLDYSGVVSSHSWSTDDAYPRIYRFGGFNAPYAGASES
                     FVKQWRKLKQVRSPKYRFGVGYGADMNGFGGQGPPRGDDQPNKVQYPFKSWDGGTTFQ
                     KAQWGERTWDINTDGVAHYGLYADWIEDLRKLAGNEIVQDMAMGVEAYLQTWERAAGI
                     RGPGCRSAHEGFAKAGNELGRVRLGESPAGVLRSANQPVSRFDRVYRWCVGDKGDRKG
                     EIAAVFTTGERVGLVSTTAPKHRYDGIGRGSKASGIRGAKRVASGLYAKRVRGGNRIV
                     YGTKRGRVTFVALATRAVGSDRAELLSFLKLARVR"
     gene            complement(8447..9244)
                     /locus_tag="VF517_07380"
     CDS             complement(8447..9244)
                     /locus_tag="VF517_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX8052798.1"
                     /translation="MIRKLLLVSALATASLALAASPSAAIVGGSDASPGEYPAVAQIS
                     YGLFSCTGTLIAPDTVLTAGHCSSVDAGTIGTPVTWPAALIDVRIGGYRDGEGERVPV
                     SSVVAHQSYLATKGYDISLLKLSRASTQPPVKVAGAGERSIWSAGTTETIVGWGVTSE
                     GGDPPAVVQEAQVPITTDAYCGGIYSDFDVRTMVCAGFPQGGVDSCQGDSGGPMFGRA
                     GDGSLRVVGTTSFGEGCARANRPGVYGRVGDDTLREWIRQRVPNGVG"
     gene            complement(9336..>9919)
                     /locus_tag="VF517_07385"
     CDS             complement(9336..>9919)
                     /locus_tag="VF517_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052799.1"
                     /translation="QAGGTGGLAGHGGGAVYLIADGAIRIAGTINASGEAGGPGVTGR
                     AGGGGGGAGGSGGTRGTAGGPGGGSFGVYLYNSTLAAIDGSQIVASNGGNGGNAGAGG
                     SGGNGGIGGLGARAELQDLGAGGNGGTGGRGGNGGAGGGGAGGPSIGVYRGGASGATV
                     VSSSISVGSGGSGGASPAGGTANGQSGVSATLF"
BASE COUNT         1335 a         3685 c         3505 g         1394 t
ORIGIN      
        1 catcggcgtc cgcccccgca cccgcggcgg cggccacccc caccgcctcc tccagctcgc
       61 ccagcggccg ccggccgcgc agcgcggtca ccgcctcatt gcgggcgatc cggtagagcc
      121 acggccgcgc cgcgatctcg cgcgtctccc cgcgcagcgc gcggtacgcc ttgagcatcg
      181 cgttctggag cgcgtcacgc gcctcctcgg ggttgccgag gatcgacagc gtgtagcggt
      241 agagcggatc ctggagccgc tcgaacagca gcgcgaacgc cttgtcgtcg ccgcgcgcgg
      301 cctccgcagc cagctcgtcg tcggacagcc gcttcagcgg cgtgaaccgg ccccgccggc
      361 cctcgctatc gctcatctct cggctctccg tcgccctgcg tcgctgcccg gtccgtcagg
      421 ctttcgcggg ataacgcgcg gaacctcctc gcgcccgcga attcacctta ccccgcgttg
      481 ggcccatgcg ccaccgcctg gggcggtggc gcgccgactc agtgctgcga gatgacccgg
      541 tcgatcaggc cgtactccag cgcctcctcg ggcgtgaagt agcggtcgcg gtccatatcc
      601 acgtggacct tctcgatgtc ctgggtcgtg tgcttggcgt agatctcgtc gagccggcgg
      661 cgcaggtcga ggatctcgcg ggcgtggatc tcgatgtccg cggactggcc ctggtagccg
      721 cccgacggct ggtggatcag gatccgcgag ttggggtggg cgaagcgctt gccgggcgtg
      781 ccaccggtca gcagcagcga gcccatcgac atggccatgc cgtagcaaat cgtgaccacg
      841 tcgggcttga tgaactgcat cgtgtcgtag atcgccaggc ccgcgtacac ggagccgccg
      901 ggcgagttga tgtagagcga cacgtccttg tcgggatcgt ccgactcgag gtgcagcagc
      961 tgggccacga tcaggttcgc gatctggtcg tcgacctggt cgccgaggaa gacgatccgc
     1021 tcgttgagca gacgcgagta gatgtcgaac gagcgctcgc cgcgcgatgt ctgctcgacg
     1081 accatcggga ccagcggcat tgacagacct ccagttcagg cttctgagtc gggccccctc
     1141 aggggcgccc gagtctatcc atggcgtacg gttcccccat ggtcgaagcg gcgggcacgt
     1201 tcgagctggg cggggagctg gaggtcaacc gcctcggctt cggcgcgatg cgcatcacgg
     1261 gcgagggcgt ctggggcgat ccgccggacc cctacgccgc gatgagcgtg ctgagccacg
     1321 cggtcgagct gggcgtgaac ctgatcgaca cggccgactc ctacggcccc gaggtctccg
     1381 agaacctgat cgcccagacg ctccacccct acgacgggat cacgatcgcg accaagggcg
     1441 gcctgacccg ccccggtccc ggcgtgtggg agcgcaacgg gcgccccgag cacctgcgct
     1501 cggcctgcga ggcgagcctg cggcgcctgc gcgtggaccg catcgacctc taccagctgc
     1561 actcgcccga ccgcgacgtc ccgtacgagg agtcggtggg ggcgctcggc gagctccagc
     1621 aggaggggaa gatcaacctg atcgggctct cgaacgtatc ggtcgagcag ctcgacgccg
     1681 ccatgggcat cgtcgacgtc gtgagcgtgc agaaccgtta caacctcacc gaccgcggca
     1741 acgaggacgt gctggacgcc tgcgaggccc gcggcatcgg cttcgtgccg tggttcccgc
     1801 tggccaccgg cagcctggcc gagcagggcg gcgtgctcga cgaggccgcc gaccgccacg
     1861 acgccacgcc ctcccagatc gcgctcgcct ggctgctcgc gcgctcgccg gtgatgctgc
     1921 cgatccccgg caccggctcg gtcgagcacc tggaggagaa ctgcggcgcg gcgcggatcg
     1981 agctggacga ggacgagctc gagcagatcg cgagcgccgc cggatgacgg ccgccaccga
     2041 gctcgacctc ccgtcgctgg agtaccagga cgccgagctg cggggggacg cgttccacga
     2101 cgcgatgcgc gagctgcgcg agcagaactg gctcgcgcgc gtggaccccg tcggcttcgc
     2161 cgtgctcgac cgcgaggccg ccgcgcactt catgcgcagc cgcagcctga ccttccccgg
     2221 ccgccggatg ctcgaggtcc agggcgtgac cgaggggccg ctctacgagc gcttcaaggg
     2281 caacctcctc gacctcgacg gcgacaacca ccggcggctg cgcaagctcg tccagccggc
     2341 gttcacgccg ccggccgccg accgccaccg gcccgtgatg cgccagctcg tggaggcgct
     2401 ccacggcagc gtggccgagg ccggccgctg cgacttcgtc cagtcgttcg cgcagcccta
     2461 ccccgcgatg atgatcgcgc acgtgatggg cgcgccgctc gaggacgcgg ggcgcctgca
     2521 cgagtgggcg aactacatcc agcagcagtt cgacccgatc aagctctcca tccagctcgc
     2581 cgagatcgag cgcgccgccg aggagttcca gcactacgcg tgggacctcc tgcgcaagcg
     2641 ccagggcgac ccgaaggacg acctgatctc cgagctgatc accgccgagg aggagggcga
     2701 ccggctcagc gaggaggagt gcgtgcacct cgtgagcgcg gtgctcgtcg ggggcgtgga
     2761 caccacgcag agccagctcg cgcacggcat gctgctgttc gcccggcacc cggaccagtg
     2821 gcgcatgctc gcggaggatc cgtcgctcgc tccgcgggcg gtcgaggaag tgctgcggct
     2881 cgacccgatc acgcccttca ccgcccgcat cgcgctcgag gacgtggagt accgcggggt
     2941 cacgttcccg aaggacacga tcgtgatcgc cgccgccgtg accgccaacc gcgaccctga
     3001 tgcctacgag gagccggagg cgttcgacat cacggccgac cgcgaccggt cgaagccgct
     3061 gacgttcggc gcaggcccgc acttctgcct cggagcgagc ctcgcgcgcg cggagctcca
     3121 ggaggcgttc gccctactcg cgccgcggat gcccgacctc gagctcgacg gcgaaccgat
     3181 cttcgacacc ccgttcgggg tgtacggcat ggagagcctg ccgatccgct ggcgggcctg
     3241 accgtcggta ggaaaagtcc tggaaaaagg tcggcccgcg gtgccgcggg gggctgcggc
     3301 accgcgggcc tggttactgc gaggcgccgg tttcgtaccc gggcgtctgg ccacctaatc
     3361 tcaccccgtg cgcaattacc tccgtttggg tggggtgctc gcggtgctcg ccctggcgtt
     3421 cgctccggga gcgcaggccg gctaccgcac ccagctcgag cgcgccgggc tgaccgatct
     3481 gcgcacccac gcgccggagc tggcggtgga catgcgctac gcgaccgacc gcaacttcac
     3541 gggcgcgccg ctgccgggct actgcgcaca ggagccctac ctgcgactgc gtgccgcccg
     3601 ggcgctcggc cgcgcccagc ggcggctgcg ctccgacggc ctcgggctga tggtgttcga
     3661 cgcgtaccgc cccgcccgcg cgagccgcgc catggtccgc tgggcccgcc ggacgggccg
     3721 cgaggacctg ctgaacggtt ggatcgccgc gcgcagcaac cacaaccgcg gcgccgccgt
     3781 ggacctcacg ctcgtccacc tcgacacggt caagccgctg gcgatgggca ccgagtacga
     3841 cgccttctcg tcgcgctccg cgaccgccgc ggtcggcggc aaggcgctcc gcaaccgcct
     3901 gcggctcaag cgcgcgctcg aggtcgaggg cttccgcaac taccgccgcg agtggtggca
     3961 ctacgacttc cccgcggagc tccgcgcccc gcggatcgac gtcgcgctcg gctgctgaac
     4021 gccgctaccc gaacggcgcg aacgccacgt cgccgagcgt gtcgaggcgg ctcgccttga
     4081 tacccgcgtc ggagacggtg aacagccggt cgcccaccac cagggcgcgg cggatgtccg
     4141 gcacgtagcc cccggagccc tgggggtgcg cgatgcgccc cacttcgtcg atgccggtcg
     4201 cgggcgcgac tccgaagccg accgccccca cgaacggctc cccgccgtcg cgcgggtagg
     4261 accggatcgg gatcacggcc agcgagcgcc ccggccacca caggaaagcg tggtggtcgt
     4321 actccgcctc gctgcccgcg ccggcgagcg tccgccggtg ggtgcgcctc ggctggcgcg
     4381 gatcggacac gtcgaagagc gagacctgcg tgccctgcgt gcggccctcc tcgctcgcgt
     4441 cctggccgac gcccagcagc aggtcgcgcc ccaccgggtg caggtaggcc gagtagccca
     4501 gcaccttcag ctcgccggcg acggcgggct tcgccggatt ggagaggtca accacgtaga
     4561 gggggtcgac ctggcggaag gtgaccacgt agccctggtc ctcgatgaag cgcaccgagt
     4621 agatccgctc gccgcgcccg agcccgccga cctggccgcg cgtgcgcagc tcgccgccat
     4681 cctcggccag cgtcgtcacg aagctctcgc tcgcgggctg cggcgccccg ccaccccacc
     4741 agagcggctc ctcggtggtg gccacgcgca ggtggtcccg gtactccgac agcgcccact
     4801 ggttgaggag gtagccgcgc acggtcccgc tcgcgcggaa ggtcgtgtgg tcgctgtcgc
     4861 gtgcgtcgaa gcggtggatc gtggtggcgc ggccgcccgg caccgactcg ccggactccg
     4921 gcgtcgggtc gagccagcgc tcgctcgcca cgtagaggct cccctgcgac gcgtagaccg
     4981 tgcgcgcatc ggtcatcagc ccgtcggagt cgaccgggac gatgcccttc gacaggtcga
     5041 tcgtgagcac ggtcagcatc ccgaggcccg agaagaccgc cggcctgcgc gtggcgcggc
     5101 agccgaccag cgagcgcgtg acggagttgc caccgcgcgc gctcgtgagc cggtagcgcg
     5161 ggatccaggt cttcaggtgc gtgcggcgga tcgccgcgcg gcgccgctgc acctccgggg
     5221 cgtcgccgtc ggcgacgggg atcggccgcg ggtaggacgc gatcaccacg cgggcggtcg
     5281 tgccgcgcat gcgcgcgctc acgtagctgc cgtccacgtc gagggtgcgc accacgcgcg
     5341 ggttcgaccg gtcgctcacg tcgatctcgg tgagcacggc ccgcgggtcc tgcgcgatca
     5401 ggtcggccgc gagcggctgc gcgtcgccgt agcccggcgt cgagaacacc atcaggcggt
     5461 cgccggcgag cagcatctcc tgcgagaacc cgccgagctt caggctgccg acgaccttcg
     5521 gctcggcgcc gcgcacgtcc accacgcgca gcgtcccgct cgcgagcgcg aacacgtgcc
     5581 ggccgtccgt tttcacgaga tccggctcgt cgactccacc ctcctgcacg ttggtggtcg
     5641 agtgctccgg cgcctccgcg gcggcgccgt cggcgggcgc ggcctgtgga gcgccggagt
     5701 cctcgcgcgg ccccgacgcg gggaacgcga tgcccggctg cgggcggcgc ggcagtccgg
     5761 ccggctccgc gatccgagcg gcctgggtgc gcgcgtgggt cagcagggcg tcgcacgagc
     5821 ggaacgcgcg cggccacgac ttcggcgtcg cggcggaacc ggtggcgggg gcgcagagcg
     5881 cgacagcgag ggcgagcacg gccgcccgcc gcacggtcac cgcacgcgag cgagcttcag
     5941 gaaactcagc agctcggcgc ggtcggagcc gacggcgcgc gtggccagcg cgacgaaggt
     6001 gaccctgccg cgcttcgtgc cgtagacgat ccggttgccg ccgcggaccc gctttgcgta
     6061 gaggcccgag gccacccgct tggctcccct gatccccgac gccttgctgc cgcggccgat
     6121 gccgtcgtag cgatgcttcg gcgcggtggt cgacacgagc ccgacccgct cgccggtcgt
     6181 gaacaccgcg gcgatctcgc ccttgcggtc gcccttgtcg cccacgcacc agcggtacac
     6241 gcggtcgaag cggctgaccg gctggttcgc gctgcgcagc acgcccgccg ggctctcgcc
     6301 gagccgcacg cgccccagct cgttgcccgc cttcgcgaag ccctcgtgcg ccgagcggca
     6361 gccgggcccg cggatccccg cggcgcgctc ccaggtctgg aggtaggcct ccacgcccat
     6421 cgccatgtcc tgcacgatct cgtttccggc cagcttgcgc aggtcctcga tccagtcggc
     6481 gtagaggccg tagtgggcca cgccgtcggt attgatgtcc caggtgcgct cgccccactg
     6541 cgccttctgg aaggtggtcc caccatccca cgacttgaac gggtactgga ccttgttcgg
     6601 ctggtcgtcg ccgcggggcg ggccctggcc gccgaagccg ttcatgtcgg cgccatagcc
     6661 gaccccgaag cggtacttcg ggctgcgcac ctgcttcagc ttgcgccact gcttgacgaa
     6721 gctctcggag gcgccggcgt agggagcgtt gaagccgccg aacctgtaga tgcgcgggta
     6781 ggcgtcgtcc gtgctccagc tatggctcga gacgactccc gagtagtcga gcgactccgt
     6841 caacgccagc gactgccgcc ggccgagcac gctcatgtgg tcggggtcga agagcatctt
     6901 gcgcttgacc agctcgcgga tcgtgaactc gcccaggtcg gtgagcccgc gcttgttgca
     6961 ctgcgggccc tcgccgtaca ggggcaggag gccgccgggt gcgtactgcg cgtatatctc
     7021 gaacagccgg tcggtgttgt ggtcgggcgt ctgcgtggcc ggcacgcggt cggagttctc
     7081 gtccttgcag tcgtcgtagt cgaagaagtt gccggtctcg cccctgttgc cgacggtcgt
     7141 gacgagtccg gtcgagcccg agtcgcccgc cacgccgacg agtgcgttgt cgaacttgtt
     7201 gacgagctcg aactggcgga gccccgcgct ctcgagctcg ttcagccagt cggtgatgac
     7261 cccgcggtcg caggccggct ggtcgttctg cacgtcgcag tcgaacggct tcgagacctc
     7321 catgcccatc acgaccgcga gcttgccctc gttgatcgtg cgccgcgcct ggaacgggtc
     7381 cttcacgatc cggaaccagc ccttgccggg tccgccgttc tgcgcgtcga tgtagcgctc
     7441 gagctcgcgg agccgctgga tctcgatccg caccgtgttc atctcgttgc aggggttgcg
     7501 cttgagcggg tagacctcgc agagctgcgc gttgtcgacc atcaggttca cgaacacccg
     7561 cagtccggag cgccaggcgc gctccaggcc cttgtagtag acctgctcgt gggtgagcga
     7621 gcggtacgcg ggccagtcct tgaaggtcgg ccagccgacc gtgtcgtgcg gcgccaccgg
     7681 gttgccgtag gacaggacgc cctcggccgc ggcgccgtag ccgccgggcc cgtggtcggg
     7741 gcagtcgacc atcgcgaacg ggacgccgta gcggtgccac ggccggccgc agcggatgct
     7801 gccgccgatg aactcgaagg ccatcatgtg aaggtgcgcg tccacgaacc cgcgcgcctc
     7861 gccgtagggc gtcgcgccgg tgtagggctt gccggtcgcg ttgacctggg cctccgggta
     7921 ggtcgcgcag ccctccgcga tctcgaacgc gaacagtgcc gcgttgcccg agccgccgag
     7981 cacgacctcg ccggccggcg agaccgacag cggcttgccc gtggagagcg acacgacctt
     8041 gaaggcgccg ccctcgacgg tcacctccca ctcggccgcc tcgctgggcg ccgtcgccgt
     8101 ctcggcccag tcgccggacc cggcggccac gaagtcctgg tccttgccgt agagcaggta
     8161 cttgccgagg tcggtcgcct gcatccgcag cggctcgcca ccgcccacgt cggccgccga
     8221 ggcgctgtag ccgccgcccg ccgctttgcc gacgaacttg ccgagcgact gggagcggag
     8281 cacgaagcac ccgttggcga gcgcgtagcg gtcgtccgca ttctgcgccg gcgcgggcgc
     8341 ggcggcggcg ctggcggccg cacacgcgag cacgagcgcg gtcagcgcca gcaccacgat
     8401 cgtccgcaag cccctcatcc ggggcggcat tgtctcaggt tgcggcttag cccacgccgt
     8461 tgggcacgcg ctggcggatc cactcgcgca gcgtgtcgtc cccgacgcgg ccgtagacgc
     8521 ccggccggtt ggcccgcgcg cagccctcgc cgaagctcgt ggtgcccacg acgcggagcg
     8581 agccgtcgcc cgcgcggccg aacatcggac cgccggagtc gccctggcag ctgtcgaccc
     8641 cgccctgcgg gaagccggcg cagaccatcg tgcgcacgtc gaagtcggag tagatcccgc
     8701 cgcagtaggc gtcggtcgtg atcgggacct gggcctcctg gacgacggcc ggtggatcgc
     8761 cgccctccga ggtgacgccc cagccgacga tcgtctctgt cgtccccgca ctccagatgc
     8821 tgcgctcgcc cgcgcccgcg accttcacgg gaggctgggt cgacgcgcgc gagagcttca
     8881 ggagcgagat gtcgtagccc ttcgtcgcga ggtagctctg gtgcgcgacg acgctgctga
     8941 ccggcacgcg ctcgccctcg ccgtcccggt agccgccgat gcgcacgtcg atgagcgcgg
     9001 cgggccaggt gaccggcgtg ccgatggtgc ccgcgtccac ggagctgcag tggccggcgg
     9061 tcaggaccgt gtccggagcg atcagcgtgc ccgtgcagga gaagaggccg tagctgatct
     9121 gagcgactgc cggatactcg cccggagagg cgtcgctgcc gccgacgatg gcggcggacg
     9181 gcgaggcggc gagcgcgagc gacgcggtcg ccagggcgga gacgagcagc agcttgcgta
     9241 tcaagacgaa gacctccggg caggggatac ctcgcgtcag gtacccagcc cggaggccct
     9301 ctagtcctcg cgcgccagac gggccgcgcg cccccctaga acagggtcgc ggagaccccg
     9361 ctctgcccgt tggccgtgcc gcccgcgggg ctcgcgccgc cgctgccgcc gctgccgacg
     9421 ctgatcgacg agctcaccac ggtcgcgccc gaggcgccgc cgcggtagac gccgatgctc
     9481 gggccgcctg caccgccgcc cccggcgccg ccgttgccgc cccgcccacc ggtgccgccg
     9541 ttgccgccgg cgccgaggtc ctggagctcg gcgcgtgcgc cgagaccgcc gataccaccg
     9601 ttgccgccgc tgccgccggc cccggcgttg ccaccgttgc cgccgttgct cgcgacgatc
     9661 tgcgagccgt cgatcgcggc cagcgtcgag ttgtagaggt agacgccgaa cgagccgccg
     9721 cccgggccac ccgcggtgcc cctggtgcca ccggagccgc cggcgccgcc gccgccaccg
     9781 cccgcgcgac cggtgacgcc cggtccaccc gcctcgccgg acgcgttgat cgtgcccgcg
     9841 atccggatcg caccgtcggc gatcaggtac accgcgccgc cgccgtgacc cgcgagcccg
     9901 ccggtaccgc ccgcctggt
//
LOCUS       DASVPL010000142         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_254030, whole genome shotgun sequence.
ACCESSION   DASVPL010000142 DASVPL010000000
VERSION     DASVPL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254030"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1165
                     /gene="plsX"
                     /locus_tag="VF517_07390"
     CDS             167..1165
                     /gene="plsX"
                     /locus_tag="VF517_07390"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX8052800.1"
                     /translation="MIALDANGADRGPRTVAEGGRDSGVPVTIFGPAADLGGFENVVD
                     APVAVTNDEDAAHAVRAKPESSIVQAAKAVGSGAAGAFVTPGNTGAALAAGLFHLKRI
                     RGVHRPAVAVRLPVPGRPVLLLDAGANVESRPEWLVQFAYMGAAYMEAVHGVERPTVG
                     LLSIGEEAKKGTDTVVEANRVLAAGGLDFAGNIEGGDVTAGTVDVVVTDGFTGNVTLK
                     AIEGTAKTVMGAVRDAVRSGPVSTLGGLLIRGKARKLRAEFDPDSAGAGILLGLRGVG
                     MVTHGSATAAGVATAVRAAHRAVEEGVVERTEAALRASGALGEAASAPAVSVADRP"
     gene            1162..1416
                     /gene="acpP"
                     /locus_tag="VF517_07395"
     CDS             1162..1416
                     /gene="acpP"
                     /locus_tag="VF517_07395"
                     /inference="COORDINATES: protein motif:HMM:NF002148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8052801.1"
                     /translation="MNREELFEVVREHLSTELSVDASAIRDDTRFKEDLQADSLHLVE
                     LMVELEDGYGVRIPDDEAARIETVGQVVDYVAAHAPERAP"
     gene            1450..2109
                     /gene="rnc"
                     /locus_tag="VF517_07400"
     CDS             1450..2109
                     /gene="rnc"
                     /locus_tag="VF517_07400"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX8052802.1"
                     /translation="MPDELGRQAFTHSSWVEQRTESYERLAFLGDVVLSVAVSDHIYP
                     RFPEWGAGRLTKLRAQAVSRTACAEVARGLGIPARLQAAAPEGVGKNAEVLVGSDRIL
                     ASVCEAVIGAAYLAFGIERVKPAVVEAFSEQLDEALDSPVDFKSVLQETLARHSEVVS
                     YRIVEASGPAHDRSFTATAEVGGEEVGRGEGKTKKAAEQEAASHALDHVAEHWPEPGG
                     G"
     gene            2112..>3349
                     /locus_tag="VF517_07405"
     CDS             2112..>3349
                     /locus_tag="VF517_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8052803.1"
                     /translation="MHLRSITVKGFKSFPDRVRLDFAPGVSVIVGPNGSGKSNVTDAV
                     LWALGEQSPLAVRGQTMQDVIFAGGHGIPSRNAAEVEVVIDNNDGGLATEFSEISIAR
                     RLDRSGEGEYRLNGARCRLVDVVEVLSDTGLGKEMHSVVSQGRVESIIHSKPKDRRLL
                     IEEAAGLGKHRKRRRRAQLKLERTADNIARALDVEREARSRLRPLKRQAEAAELHARL
                     ERQSLEARLELARDAAGAAARELEAAEAAARAAGAKRDEAERLLSAVAVRREQAEEAF
                     AAASRYREALAGRVFAARSAHDRIGMRHERALDLARRSAAAGAQAAAEREALAERDAA
                     AEPEAPEAAGRVAELESELEKVERELARNLDEELAELDAERERAAARAEGLEGAVAER
                     REALDATERAADEARRARVEA"
BASE COUNT          450 a         1183 c         1319 g          397 t
ORIGIN      
        1 gtcccgggac ctcgcgcccc cggatgccgc tgcaaacctt cgccggacct gcgtcctcga
       61 gttcgtccat gcacctgtgc atggacttca ccctgcgtcc ttggccagct cggttttcgc
      121 ggccccgagc gcacgaggcc ccggaaccga tcaccgtggc ggcgggatga tcgccctcga
      181 cgccaacggg gcggatcgag gaccgaggac ggtcgccgag ggcggccgcg actccggcgt
      241 gccggtcacg atcttcggcc ccgcggcgga tctcgggggc ttcgagaacg tcgtcgacgc
      301 gccggtcgcg gtgaccaacg acgaggacgc cgcacacgcc gtgcgcgcca agccggagtc
      361 ttcgatcgtc caggccgcca aggcggtcgg ctccggcgcg gcgggcgcgt tcgtgacccc
      421 cggcaacaca ggcgccgcgc tcgccgccgg gctcttccac ctcaagcgca tccgcggcgt
      481 gcaccggccc gccgtggcgg tgcggctgcc cgtgcccggg cgccccgtgc tgctgctcga
      541 cgcgggcgcg aacgtcgagt cgcgccccga gtggctcgtc cagttcgcct acatgggcgc
      601 cgcctacatg gaggcggtgc acggcgtgga gcgccccacg gtgggcctgc tctcgatcgg
      661 ggaggaggcg aagaagggca ccgacaccgt ggtcgaggcc aaccgcgttc tcgccgcggg
      721 cggtctcgac ttcgcgggca acatcgaggg aggcgacgtg accgcgggca cggtcgacgt
      781 ggtggtcacc gatggcttca ccggcaacgt gaccctgaag gcgatcgagg gcaccgccaa
      841 gacggtcatg ggagccgtgc gcgacgcggt ccgttccggc ccggtgtcca cactcggcgg
      901 cctgctgatc cgcggcaagg cgcgcaagct gcgggcggag ttcgacccgg acagcgcggg
      961 cgccggcatc ctgctcggcc tgcgcggcgt ggggatggtc acccacggaa gcgccacggc
     1021 ggccggggtg gccacggccg tgcgcgcggc gcaccgggcc gtcgaggagg gcgtggtcga
     1081 gcggaccgag gccgcgctgc gcgccagcgg ggccctgggc gaggccgcgt ccgcacccgc
     1141 tgttagcgtc gcggaccggc catgaatagg gaggagctgt tcgaagtggt gagggagcac
     1201 ctctccaccg agttgtcggt ggacgcgtcc gcgatccgcg acgacacgcg cttcaaggag
     1261 gacctccagg cggactccct gcacctggtc gagctgatgg tcgagctgga ggacggctac
     1321 ggcgtccgga tccccgacga cgaggcggcg cgcatcgaga cggtcggcca ggtggtcgac
     1381 tacgtcgcgg cgcacgcacc cgagagagct ccctgatccc tggactcgct ccagtcgctc
     1441 ctcgaggagt tgcccgacga gctggggcgt caggcgttca cgcactcgtc gtgggtcgag
     1501 cagcggaccg agtcctacga gcggctggcg ttcctgggtg acgtggtgct gagcgtggcg
     1561 gtctccgacc acatctaccc gcgtttcccg gagtggggcg ccggacggct cacgaagctg
     1621 cgcgcgcagg cggtctcgcg cacggcgtgc gcggaggtgg cgcgcgggct cggcatcccc
     1681 gctcgcctgc aggccgccgc gcccgagggg gtcgggaaga acgccgaggt gctggtgggc
     1741 agcgaccgga tcctcgcgtc cgtgtgcgag gcggtcatcg gcgccgcgta cctggcgttc
     1801 gggatcgagc gcgtcaagcc ggcggtggtc gaggcgttct ccgagcagct cgacgaggcg
     1861 ctcgacagcc cggtcgactt caagtccgtg ctccaggaga cgctggcgcg gcactcggag
     1921 gtcgtgagct accggatcgt cgaggccagc ggccccgcgc acgaccgcag cttcaccgcc
     1981 acggccgagg tcggcggcga ggaggtcggg cgcggcgagg gcaagaccaa gaaggccgcg
     2041 gagcaggagg cggcgtcgca cgcgctcgac cacgtggccg agcactggcc ggagccggga
     2101 ggcggctagc ggtgcacctg cgctcgatca ccgtcaaggg cttcaagtcg ttcccggacc
     2161 gggtgcggct cgacttcgcg ccgggcgtct cggtgatcgt cggcccgaac ggctccggca
     2221 agtcgaacgt gaccgacgcg gtcctgtggg cgctcggcga gcagagcccg ctggcggtgc
     2281 gcggccagac gatgcaggac gtgatcttcg ccggcgggca cggcatcccg tcgcgcaacg
     2341 cggccgaggt ggaggtcgtg atcgacaaca acgacggcgg cctcgcgacc gagttctccg
     2401 agatctcgat cgcccgccgg ctcgaccgct ccggcgaggg cgagtaccgc ctgaacggcg
     2461 cgcgctgccg gctggtggac gtggtggagg tcctctccga caccgggctc ggcaaggaga
     2521 tgcactcggt ggtctcgcag ggccgggtcg agtcgatcat ccactcgaag ccgaaggacc
     2581 ggcggctgct gatcgaggag gccgccgggc tcggtaagca ccgcaagcgc cgccgccggg
     2641 cgcagctcaa gctcgagcgc accgcggaca acatcgcgcg cgcgctcgac gtggagcgcg
     2701 aggcgcgctc gcggctgcgg ccgctgaagc gccaggccga ggcggcggag ctgcacgcgc
     2761 ggctcgagcg ccagtcgctc gaggcgcgcc tggagctggc ccgcgacgcc gccggcgccg
     2821 cggcgcggga gttggaggcc gccgaggccg ccgcgcgcgc ggcgggcgcg aagcgcgacg
     2881 aggccgagcg gctgctcagc gcggtggccg tgcgccgcga gcaggccgag gaggcgttcg
     2941 ccgccgccag ccgctaccgc gaggcgctcg cgggccgcgt gttcgcggcg cgctcggcgc
     3001 acgaccggat cggaatgcgc cacgagcgcg cgctcgacct cgcgcgccgc agcgccgccg
     3061 ccggcgcgca ggccgccgcc gagcgcgagg cgctcgcgga gcgggacgcc gcggccgagc
     3121 ccgaggcgcc cgaggcggcg ggccgggtcg ccgagctcga gtcggagctg gagaaggtcg
     3181 agcgcgagct ggcgcgcaac ctcgacgagg agctcgccga gctcgacgcc gagcgcgagc
     3241 gcgccgcggc ccgcgcggag gggttggagg gcgccgtggc cgagcgccgc gaggccctcg
     3301 acgccaccga gcgcgcggcc gacgaggcgc gccgcgcccg cgtggaggc
//
LOCUS       DASVPL010000143         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_291691, whole genome shotgun sequence.
ACCESSION   DASVPL010000143 DASVPL010000000
VERSION     DASVPL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291691"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1423)
                     /locus_tag="VF517_07410"
     CDS             complement(2..1423)
                     /locus_tag="VF517_07410"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX8052804.1"
                     /translation="MARDEEQIPTGRVARTAKVGTVIGTQGARYAGTRAANVVRSKER
                     ATEKLDQRHLEAAERMVDTLGTMKGAAMKIGQLASFIDTEFLPPEYRELYQDKLSSLR
                     TSAPPMPWAKVVKVLEEEWDEPPEELFEDFEHEAAAAASIGQVHRATLPDGRRVAVKI
                     QYPGVAEALRADMQNAGMILRLAKALAPGLDAKAAAAELKERVLEELDYEYEAQNQRT
                     FARAYRGHPFVFVPDVVSRLTTERVLVTEWVDGIGFDQVKELPQEDRSRFGEIVFRFC
                     FGSIYHLQHFNADAHPGNYLLMPDGKVAFLDFGMTKQLDKEQIELEIAALQAVFDDDP
                     EELVGALHDLGFLRNPKRVDAERMMEHVKTVGGWYMEDRELTVDSELVMRAIAAVTDP
                     RSDFYDLMRRENVPANELMGRRMESGVLAVLGQLNATRNWDRIGREWWFAEDPATDLG
                     REEWAFFESKGEKRVRKFTARTP"
     gene            1464..>1666
                     /locus_tag="VF517_07415"
     CDS             1464..>1666
                     /locus_tag="VF517_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052805.1"
                     /translation="MATAKRPKLAVEERPEHGSRATRRLRRAGYVPGIVYGGGDGACT
                     SFKVGVRDLRAALVASSAVLDFE"
BASE COUNT          221 a          608 c          568 g          269 t
ORIGIN      
        1 gtcagggcgt gcgcgcggtg aacttgcgga cgcgcttctc gcccttcgac tcgaagaacg
       61 cccactcctc gcgcccgagg tcggtcgccg gatcctcggc gaaccaccac tcgcggccga
      121 tccggtccca gttgcgcgtg gcgttcagct gcccgagcac ggccagcacg ccggactcca
      181 tgcgccggcc catcagctcg ttggcgggca cgttctcgcg cctcatgagg tcgtagaagt
      241 ccgagcgcgg gtcggtaacc gcggcgatcg cgcgcatcac gagctcggag tccaccgtca
      301 gctcgcgatc ctccatgtac cagccgccga cggtcttcac gtgctccatc atccgctcgg
      361 cgtccacgcg cttcgggttg cgcaggaagc ccaggtcgtg cagcgcaccc accagctcct
      421 cggggtcgtc gtcgaacacc gcctgcagcg ccgcgatctc gagctcgatc tgctccttgt
      481 cgagctgctt ggtcatcccg aagtcgagga acgcgacctt gccgtcgggc atcagcaggt
      541 agttgccggg gtgcgcgtcg gcgttgaagt gctggaggtg gtagatcgag ccgaagcaga
      601 agcggaagac gatctcgccg aagcggctgc ggtcctcctg gggcagctcc ttcacctggt
      661 cgaagccgat cccgtcgacc cactcggtca ccagcacgcg ctcggtggtc aggcgcgaga
      721 ccacgtccgg gacgaagacg aacgggtggc cgcggtaggc gcgcgcgaac gtgcgctggt
      781 tctgggcctc gtactcgtag tcgagctcct cgagcacgcg ctccttcagc tccgcggcgg
      841 cggccttggc gtcgagcccg ggcgcgagcg cctttgccag ccgcaggatc atcccggcgt
      901 tctgcatgtc cgcgcgcagc gcctcggcga cgcccgggta ctggatcttc acggccacgc
      961 ggcggccgtc ggggagcgtg gcgcggtgca cctggccgat cgaggcggcg gccgcggcct
     1021 cgtgctcgaa gtcctcgaac agctcctcgg gcggctcgtc ccactcctcc tcgagcacct
     1081 tcacgacctt ggcccacggc atcggcggcg ccgacgtcct gagcgagctg agcttgtcct
     1141 ggtacagctc gcggtactcg ggcgggagga actccgtgtc gatgaacgag gcgagctggc
     1201 cgatcttcat cgccgcgccc ttcatcgtcc ccagcgtgtc caccatccgc tcggcggcct
     1261 cgaggtggcg ctggtcgagc ttctcggtgg cgcgctcctt cgaccggacg acattcgccg
     1321 cgcgcgtgcc cgcgtagcgc gcgccctgcg tgccgatcac ggtgccgacc ttggcggtgc
     1381 gagccacgcg accggtcggg atctgctcct cgtcacgcgc catgggcgcg agcgtaccgg
     1441 tccgcactac actggcccgc cgcatggcta ccgcgaagcg ccccaagctg gcagtcgagg
     1501 agcggcccga gcacggctcg cgcgccacgc gcaggctgcg ccgcgcgggc tacgtgcccg
     1561 gcatcgtgta cggcggcggc gacggcgcct gcacctcctt caaggtcggc gtgcgcgacc
     1621 tgcgcgcggc gctcgtcgcg agctccgcgg tgctcgactt cgagat
//
LOCUS       DASVPL010000144        11334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_330416, whole genome shotgun sequence.
ACCESSION   DASVPL010000144 DASVPL010000000
VERSION     DASVPL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330416"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VF517_07420"
     CDS             <1..261
                     /locus_tag="VF517_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052806.1"
                     /translation="VLRLATVAVAEREGWERDRVLEALDGLPGAERVEFFEMPRLDVS
                     SSLVRRRAAEGLPVRYFVPDLVAEEIAERGLYTGAGAAVSAR"
     gene            258..599
                     /gene="rsfS"
                     /locus_tag="VF517_07425"
     CDS             258..599
                     /gene="rsfS"
                     /locus_tag="VF517_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX8052807.1"
                     /translation="MTSRELAERIAAIASDRKATDIRMLEVSGLVGYTDWFVICTGNT
                     ERQTKAIRDAVREELKRDGHLPTRVEGEREARWILMDYLDCVLHIFTPDARSYYRLEQ
                     LWGEAPAEQVS"
     gene            1052..1124
                     /locus_tag="VF517_07430"
     tRNA            1052..1124
                     /locus_tag="VF517_07430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1085..1087,aa:Ala,seq:ggc)
     gene            1493..4225
                     /locus_tag="VF517_07435"
     CDS             1493..4225
                     /locus_tag="VF517_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8052808.1"
                     /translation="MRRGTGGFWKPAAVAAVLALAAPGVAVAQEPLTVPAFEGFNSVL
                     AQGEGESIEAPELAANQASGALPDTFVNQQPLYVDIMPRAATLGPGDIGRFYKDSRFG
                     AMPGGVGSTTTPRPGVRILRDARFGVAHIYGDTREDLMFGNGYATAQERLFMMDAIRR
                     TARGTLAGLTGASAAEGDAEQLTDQDFSGEELTRQFEALGQRWGGPGERAKADILNYV
                     AGINKRIDEVNADPTQMPAEYPALGARPEPWTVADSVAMATLLVTQFTVSNGGEEVNS
                     QLRQGFERKFGKGWQRPYRDLREADDPEALTVSPHKHLSDRPGRLQSGRNAIPDFGSI
                     RPRDPQIEGPGAGDAQAASGAGLPAWVHAVNRLKKALPDEESNAVIVPRKLSSTGHAL
                     YAAGPQVDYWSPQIFVEQELHGGGIDVEGVSFPGASPWPLIGHGVDFVWSGTSANGDN
                     QDTFVESLCNPDGSAPSDRSTHYTYRGQCRPFTTRDQTVTTPVSPIDPSPPERITQRT
                     MRSVHGPVFAFATVGGKPVALAKAKGVDFHELDASIPFMRLAENEPRSLRAFMKVMSP
                     FPGTENWFYANRDEVGFVQSGNFQKHAPGSDVDLPYNGDGSGDWVGFDPDTYSFETIP
                     PANRPHAANEASISWNNKEGIGWRKGPTEWSDGPVHHALLLRRRLEDEQRVKGGKVDL
                     VGLTRAVNRAATGDLRPEEPYRWMRQVIGRAQGRDEKMLRLIDAWRADGGNRLDKDGD
                     NVYEHSAAVILMDAWWPRFVQQAFQPELGPDLFGAVEDRVLGLGGFGWGWASHVQKDL
                     RNVLGRKVRGRYSRFYCGGRPTVKPLTGSGRAVALKRCRAILLHSLRQAVQGISNDRG
                     ADVAEWKYPATCPLTRPRACVQKEPTAAGAVETPPFPWQNRGTFHQVTEVLGRR"
     gene            4222..5142
                     /locus_tag="VF517_07440"
     CDS             4222..5142
                     /locus_tag="VF517_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HEX8052809.1"
                     /translation="MNQLAQEAVDAINEVSGRFDGHRAAHAKGTLCAGTFTPTPEAAE
                     LSPAAHFREPVRVTVRLSNGSGNPHAPDGERTEGRGIAIKFYLPDGGTTDIVGLTLPV
                     FFVRDPDAFVEFVRARKPDPETGQPDMQKIGAFLGEHPETGAALQLILPSMVPPRSYA
                     TCAFNSLHAFRLGERWARYRIEPEAGVEHVPEEEIDGLAEDYLQTELRERLEAGPVRF
                     RLYARLGEEGDPTDDPTVAWPDERERVELGTLELTGLDTTREQDGDVLVFDPTRVTDG
                     IELSDDRILRFRADAYATSVFRRTGVSRDD"
     gene            complement(5156..5605)
                     /locus_tag="VF517_07445"
     CDS             complement(5156..5605)
                     /locus_tag="VF517_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052810.1"
                     /translation="MIRRATVGAVLSAALVLPAGALAAADNYRGKTNQGRKASARVVD
                     GRVKLVKLVWRVPCRKKDMVFDNATNWTDRPEGPIEQPGDGTFTDSGRTKGDIGGGER
                     ADAKLTLSGRFDGTHMVGKSTITAKIYRSGKQIDTCKGTIRFDIPRV"
     gene            complement(5652..6221)
                     /locus_tag="VF517_07450"
     CDS             complement(5652..6221)
                     /locus_tag="VF517_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8052811.1"
                     /translation="MSPLGVARPLPRVLDPLSDGEVSVREWRREDLHAMAAMLDDEPI
                     ARWTRVPQPYTERDGLDYFARGDAERERGEGINLAVLDAGGTELLGSISLRLASWADL
                     RGQLGYLVGAHARGRGVMPRAIRLVTSWGFSALQLERIEILVHPDNAPSQRAAEKAGF
                     HREGILRSYTRVKDQRFDMWSFSALPGEL"
     gene            complement(6292..6921)
                     /locus_tag="VF517_07455"
     CDS             complement(6292..6921)
                     /locus_tag="VF517_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052812.1"
                     /translation="MASPSPSSRAAELAAEQVQAIVLAAEQAADEIRSKAERDARERE
                     REAEREGQRLRDEARATGAHELKAAEEEAARILEIARKEADDRVVRAQQAADEALAQA
                     EAISTGLRRLGESLEGQAERILRDVQAGHRRLMADLRVDPAREAPPARAPRPARDPVA
                     ADDRDAELLSTLRASQSRRGAGAAGEGEGARQRGGNPFDELEVPRWGEP"
     gene            7051..7521
                     /locus_tag="VF517_07460"
     CDS             7051..7521
                     /locus_tag="VF517_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEX8052813.1"
                     /translation="MTPAYVAVVGSGEASAADAAVAEEVGRGLAARGAVVVCGGLGGV
                     MEAACRGARGEGGVTVGLLPGPDRGAANPYVSVAIATGMGEARNALVVRAADVLVAVG
                     GSWGTLSEIALAMRVGKRVVGVGTWELARAGGEPVEGIERAADAAEAVGLALGG"
     gene            complement(7651..7923)
                     /locus_tag="VF517_07465"
     CDS             complement(7651..7923)
                     /locus_tag="VF517_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052814.1"
                     /translation="MPAAAIPVLMLLLVAAIVAATLSIGAPILALPIVFLALVIWGGG
                     RVGTARGQRRALGEDEEPIEFTAEDRRTLTPPSSTPPGRPGSEPAS"
     gene            7971..8246
                     /locus_tag="VF517_07470"
     CDS             7971..8246
                     /locus_tag="VF517_07470"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX8052815.1"
                     /translation="MTERERRRVVVHGEVQGVFFRDSTREQAERRGVGGWVTNRDDGS
                     VEAVFEGEPDAVAALVEFCRAGPSQASVERVDETSEEPEGLEDFRVV"
     gene            8314..8994
                     /locus_tag="VF517_07475"
     CDS             8314..8994
                     /locus_tag="VF517_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX8052816.1"
                     /translation="MELDREKLRVAAWLAAAVVVALLAVRALTGGDGGASESVTVDPA
                     GSAAESPGAAAPGGGGPGAGASGGGVPGAPVAGAPVFVHVAGEVRRPGVYRLPRGARV
                     NDAVQLAGGLTRRAEQTGVNLVARVSDGQQVIVPRRGAAAPGATGATPGSAGSAGGGA
                     APGAKVSLAAATPEQLDGIDGIGPTLAKRIVEYREQHGGFHSVDELRQVDGIGEKRFE
                     SLREAVTP"
     gene            9354..>11334
                     /locus_tag="VF517_07480"
     CDS             9354..>11334
                     /locus_tag="VF517_07480"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX8052817.1"
                     /translation="MPPGILPAPAAHAAPAATGLAAIVAVAAVAGLVIGSARIASIDQ
                     TAERALVSERFEGRAILPQRPRRTRFGSWAFVELAAGPANGSRVVARAGEGLGWPSHG
                     EPGIELFVAGSIERPRRTPGSRLDWPAYLKRRGVAAELDLDALRPTGGRRGGVRGWID
                     GIRRRAERGIVAGVAPDRAEVARGMVLGQDEMIDPLVVDDFRRSGLGHLLAVSGQNVV
                     LLCALAIPLIAALGGGIRARVAVLSVLVLLYVLVAGAGPSLQRAAAMAVVGLAALAVG
                     RARSRWYALLLAAAITLAVDPRAAAEPGWQLSFAAVAGILLLAPPLSRTLRALPRPLA
                     DGAAMTLAATVATGPIAAHHFEAVSLAGLVANLAALAVVAPIVWLGMVQAALGIAAGA
                     VPGELPALDALAMRAAELLGHVNDAFVGALIRIATEFAALPGAQLRLPLASPVRVIAA
                     YAVPALAWLALRALIRADAGSWAKLGVKYDQKMAQVGPGPRTRKGLARAARATHATRA
                     GLVAVDATARAAAAAWRRAALPKRAAAVALVAAGVALLWQHAAGPAAPPDRLTVSFLD
                     VGQGDATLIQDGHGAAVLFDGGPPEARTYRLLRRAGVRRLALVVATHASRDHHGGLAE
                     VLRRIPTQALLDGGDGTRDPSFAQMLEEARRRGVRH"
BASE COUNT         1431 a         4175 c         4224 g         1504 t
ORIGIN      
        1 gtgctgcgcc tcgcgacggt ggcggtggcc gagcgcgagg ggtgggagcg cgaccgcgtg
       61 ctggaggccc tcgacgggct ccccggcgcc gagcgggtcg agttcttcga gatgccgcga
      121 ctcgacgtgt cctcgtcgct cgtgcgccgg cgcgcggcgg aggggctgcc cgtgcgctac
      181 ttcgtgcccg acctcgtggc cgaggagatc gccgagcgcg ggctctacac gggggcggga
      241 gcggcggtga gcgcgcgatg acctcgcgcg agctggcgga gcggatagcg gcgatcgcgt
      301 cggaccgcaa ggccaccgac atccgcatgc tcgaggtgtc ggggctggtc ggctacaccg
      361 actggttcgt gatctgcacg ggcaacaccg agcgccagac gaaggcgatc cgcgacgccg
      421 tgcgcgagga gctcaagcgc gacgggcacc tcccgacccg cgtggagggc gagcgcgagg
      481 cgcgctggat cctcatggac tacctcgact gcgtcctgca catcttcacg cccgacgcgc
      541 gctcgtacta ccggctcgag cagctctggg gcgaggctcc ggcggagcag gtcagctagc
      601 cccgcgccgc ggccggcgcc cgtccggcat acgaactagt gttcgtgtcg tgaatccgaa
      661 cctggcaggc gcgaccgccg agagcgcgat agccgcggag gcggtgcgcc taggtttcga
      721 tgtcttcgtt ccgcccttcg ggtcaccgcg ggccgacttg gtcctaggga tctgcgagcg
      781 cctgcttcgc gtccagtgca agacggcacg actccgcgac ggcgcggtcg tgctgcgagg
      841 gcagacctgt cgtcggacgg cgacgggttt caagcacacc acctacacgc cagcggagat
      901 cgatgtgatc gccggatatt gcccggagct caggcgttgc ttcgccgtgc cgatgagcta
      961 tttcggcgac cgagtgcaga tgcatctgcg cctcgagtcg gcgagaaacg gtcagcgagc
     1021 ggggctacac ttcgcccgcg actacgagct cggggctata gctcagctgg aagagcgttc
     1081 ggctggcagc cgaaaggtcg ggggttcgag tccccctagc tccactccca ccggcgccct
     1141 cgcggcgccg gtcgtcgtcg gggcccacga gttccgcaac cgcttcggct ggtacatgga
     1201 gcgcgcggcg ggcggggagg agatccgagt tgctcaccgt gggcggccgc ggatccgcgt
     1261 ggcgccgatc gcggcataga cgcagtgttc gcgaggatcc accgctactc ggcgtcgcta
     1321 ctcggcgccg gtctcgggcg tcgtgcgggg cggcgcgacg tccgcggcgc cgcaggtggg
     1381 atcggtctcg cggacccgca cggactcggg catcagttcg aggcgcgtga tgtggaggcc
     1441 gggaacggtc gcaggctaca gcgggggtcg acatttgaca gagttcgcgg ggatgcggag
     1501 ggggaccggg gggttctgga agccggcggc ggtcgccgcc gtactggcgc tcgcggcgcc
     1561 gggcgtggcg gtcgcccagg agccgctcac ggtcccagcc ttcgagggct tcaactcggt
     1621 gctcgcgcag ggcgagggcg agtcgatcga ggcgcccgag ctggccgcca accaggccag
     1681 cggcgcgctg ccggacacgt tcgtcaacca gcagccgctg tacgtggaca tcatgccgcg
     1741 tgccgccacg ctcggcccgg gcgacatcgg gcgcttctac aaggactcgc gcttcggcgc
     1801 gatgcccggc ggggtcggct cgaccaccac gccgcgcccc ggcgtgcgca tcctgcgcga
     1861 cgctcgcttc ggcgtcgctc acatctacgg cgacacgcgc gaggacctga tgttcgggaa
     1921 cggctacgcc accgcgcagg agcggctctt catgatggac gcgatccgcc gcaccgcgcg
     1981 cggcaccctc gccggcctca ccggcgcgag cgccgccgag ggcgacgccg agcagctcac
     2041 cgaccaggac ttctccggtg aggagctgac ccggcagttc gaggcgctcg gccagcgctg
     2101 gggcggcccc ggcgagcgcg cgaaggccga catcctcaac tacgtcgcgg ggatcaacaa
     2161 gcgcatcgac gaggtcaacg ccgaccccac ccagatgccc gccgagtacc cggcgctggg
     2221 cgcgcgcccg gagccctgga ccgtcgccga cagcgtcgcg atggccacgc tgctcgtcac
     2281 gcagttcacg gtctcgaacg gcggggagga ggtcaactcg cagctccggc agggcttcga
     2341 gcgcaagttc ggcaagggtt ggcagcgccc gtaccgcgac ctgcgcgagg ccgacgaccc
     2401 ggaggcgctc acggtctcgc cccacaagca cctgagcgac cgcccgggcc gcctccagtc
     2461 ggggcgcaac gcgatccccg acttcggctc gatccgcccg cgcgacccgc agatcgaggg
     2521 ccccggggca ggtgacgccc aggcggcgtc aggcgccggg ctgcccgcct gggtccacgc
     2581 cgtgaaccgc ctgaagaagg ccctgcccga cgaggagtcg aacgcggtca tcgtcccgcg
     2641 caagctctcc tcgaccgggc acgcgctgta cgcggcgggc ccgcaggtcg actactggtc
     2701 gccgcagatc ttcgtcgagc aggagctgca cggcggcggc atcgacgttg agggcgtgag
     2761 cttcccgggc gcctccccgt ggccgctgat cggtcacggc gtcgacttcg tgtggagcgg
     2821 gacgagcgcc aacggcgaca accaggacac cttcgtcgag tctctctgca accccgacgg
     2881 ctcggccccg agcgacagga gcacgcacta cacctacagg ggccagtgcc ggccgttcac
     2941 cacgcgcgac cagaccgtca cgaccccggt ctcgccgatc gacccgagcc cgccggagcg
     3001 gatcacccaa cgcacgatgc gctccgtgca cggcccggtg ttcgcgttcg cgaccgtcgg
     3061 cggcaagccg gtcgcgctcg cgaaggcgaa gggggtcgac ttccacgagc tcgacgcgtc
     3121 gatcccgttc atgcgcctcg ccgagaacga gccgaggagt ctgcgcgcgt tcatgaaggt
     3181 gatgagcccg ttcccgggca ccgagaactg gttctacgcc aaccgcgacg aggtcggctt
     3241 cgtccagtcc ggcaacttcc agaagcacgc gccgggcagc gacgtcgacc tgccctacaa
     3301 cggcgacggg agcggggact gggtcggctt cgatcccgac acctacagct tcgagacgat
     3361 cccgcccgcg aaccggccgc acgccgcgaa cgaggcgtcc atctcgtgga acaacaagga
     3421 ggggatcggc tggcgcaagg ggccgaccga gtggagcgac ggcccggtcc accacgcgct
     3481 gctgctccgg cgccggctcg aggacgagca gcgcgtgaag ggcgggaagg tggacctcgt
     3541 cgggctcacg cgcgcggtca accgcgcggc gaccggcgac ctgcgcccgg aggagccgta
     3601 ccgctggatg cgccaggtga tcggccgagc acagggccgc gacgagaaga tgctgaggct
     3661 gatcgacgcg tggcgcgccg acggcggcaa tcgtctcgac aaggacggcg acaacgtcta
     3721 cgagcacagc gccgcggtga tcctgatgga cgcgtggtgg ccgcgcttcg tccagcaggc
     3781 gttccagccg gagctcggcc cggatctctt cggcgccgtc gaggaccggg tgctggggct
     3841 cggcggcttc ggctggggct gggcctcgca cgtgcagaag gacctgcgca acgtgctcgg
     3901 gcgcaaagtc cgcggccgct actcgcgctt ctactgcggc gggcggccga cggtcaagcc
     3961 cctgacgggc agcggccgcg cggtcgcgct gaagcgctgc cgggccatcc tgctgcattc
     4021 gctgcgccag gcggtgcagg ggatcagcaa cgaccgcggc gccgacgtcg ccgagtggaa
     4081 gtaccccgcc acctgcccgc tcacgcggcc gcgggcgtgc gtgcagaagg aaccgaccgc
     4141 ggccggtgcc gtggagacgc cgccgttccc gtggcagaac cgcggcacct ttcaccaggt
     4201 cacggaggta ttgggacgcc gatgaatcag ctcgcacaag aggccgtaga cgcgatcaac
     4261 gaggtgagcg gccgcttcga cggccaccgc gccgcccacg ccaagggcac cctgtgcgcg
     4321 ggcacgttca cgccgacgcc ggaggcggcg gagctctctc ccgccgcgca cttccgcgag
     4381 ccggtgcgcg tgaccgtccg gctctcgaac ggaagcggca acccgcacgc gcccgacggc
     4441 gagcgcaccg agggtcgcgg catcgccatc aagttctacc tgccggacgg ggggacgacc
     4501 gacatcgtcg ggctcaccct gccggtgttc ttcgtccgcg atccggacgc gttcgtcgag
     4561 ttcgtgcgcg cgcgcaagcc ggacccggag accggccagc cggacatgca gaagatcggc
     4621 gcgttcctcg gcgagcaccc cgagaccggc gccgcgctcc agctcatcct gccctcgatg
     4681 gtgccgccgc gcagctacgc cacctgcgcg ttcaactcgc tgcacgcgtt ccggctcggg
     4741 gagcgctggg cccgctaccg gatcgagccc gaggccgggg tcgagcacgt gcccgaggag
     4801 gagatcgacg ggctggcgga ggactacctg cagaccgagc tgcgcgagcg gctcgaggcc
     4861 gggccggttc gcttccggct gtatgcgcgg ctgggcgagg agggcgaccc gaccgacgac
     4921 ccgaccgtgg cgtggcccga cgagcgcgag cgggtggagc taggcacgct ggagctgacc
     4981 gggctcgaca ccacgcgcga gcaggacggc gacgtgctcg tcttcgatcc gacgcgggtc
     5041 accgacggca tcgagctgtc cgacgaccgg atcctccgct tccgcgccga cgcctacgcg
     5101 acgtcggtgt tccgccgcac cggcgtgtcg cgggacgact gagcggacgc ctgcgctaca
     5161 cgcgcgggat gtcgaagcgg atcgtgccct tgcaggtgtc gatctgcttc ccgctcctgt
     5221 agatcttcgc cgtgatcgtg ctcttgccga ccatgtgagt gccgtcgaac cggccgctga
     5281 gcgtcagctt cgcgtccgct cgctcaccgc cgccgatgtc gcccttggtc cggccggagt
     5341 cggtgaacgt cccgtcgccc ggctgctcga tcggtccctc cgggcggtcg gtccagttgg
     5401 tcgcgttgtc gaagaccatg tccttcttcc ggcacggcac cctccacacg agcttgacga
     5461 gcttgacccg cccgtcgacc acccgcgccg acgccttcct gccctggttc gtcttgccgc
     5521 gatagttgtc cgccgcggcg agcgcgccgg ctggcagcac gagagcggcg ctcagcacgg
     5581 ccccgacggt ggctcggcga atcacgcgca cgtcgtaccc gccccgacct ttgctcaatc
     5641 gagtaaaagc gctagagctc gccgggcagc gccgagaacg accacatgtc gaagcgctgg
     5701 tccttcacgc gtgtgtagga gcgcaggatc ccctcgcggt ggaagcccgc cttctcggcg
     5761 gcgcgctgcg acggggcgtt gtcggggtgc acgaggatct cgatgcgctc gagctggagc
     5821 gcgctgaacc cccaggaggt cacgaggcgg atcgcgcgcg gcatcacgcc gcggccgcgg
     5881 gcgtgcgctc cgacgaggta gccgagctgg ccgcgcaggt ccgcccatga ggcgagccgc
     5941 agcgagatcg acccgagcag ctccgtcccg ccagcgtcca gcacggcgag gttgatgccc
     6001 tcgccgcgct cgcgctcggc gtcgccgcga gcgaagtagt cgagcccgtc gcgctcggtg
     6061 tagggctggg ggacgcgggt ccagcgggcg atcggctcgt cgtcgagcat cgccgccatg
     6121 gcgtggaggt cctcgcggcg ccactcgcgc acggagacct ctccgtcgga gagcggatcg
     6181 agcacgcgcg gcagcggccg cgcgacgccg agcggggaca cgccgctcag tggaggcgcc
     6241 ccgcgccgct cacggctcgc cccagcgcgg tgccgtgtcg ctcggctccg ctcacggctc
     6301 gccccagcgc ggcacctcga gctcgtcgaa cgggttcccg ccgcgctgcc gcgcgccctc
     6361 gccctccccg gcggccccgg caccccggcg cgactggctc gcgcgcagcg tcgagagcag
     6421 ctcggcgtcg cggtcgtcgg ccgccaccgg atcgcgggcc gggcgtggag cgcgagccgg
     6481 cggcgcctct cgagccgggt ccacgcgcag gtcggccatc agccgccggt gacccgcctg
     6541 tacgtcgcgc aggatccgct cggcctggcc ctcgagcgac tccccgagcc gtcgcagccc
     6601 ggtcgagatc gcctcggcct gggccagagc ctcgtcggcc gcctgctgcg cacgcaccac
     6661 gcggtcgtcg gcctccttgc gcgcgatctc gaggatccgc gccgcctcct cctcggccgc
     6721 cttcagctcg tgcgcaccgg tggcgcgggc ttcgtcgcgc agtcgctgac cctcgcgctc
     6781 ggcctcgcgc tcgcgctcgc gcgcgtcgcg ctcggccttc gaccggatct cgtcggcggc
     6841 ctgctcggcc gcgagcacga tggcctggac ctgctcggcc gcgagctcgg ctgcccgcga
     6901 ggatgggctc ggggacgcca tggcgaaacc gatgttccca gacgcgcgcg acggaatcac
     6961 gccgttgggc gctcgcgccc gcgccggtag cctcgcgacc cccatgaagc gatacgagcc
     7021 gaagcggatc gagcccagtt ggctacggcg gtgacgccgg cctacgtggc ggtggtgggg
     7081 tcgggtgagg cgtcggcggc cgacgccgcc gtggccgagg aggtcggccg cgggctcgcg
     7141 gcgcgcgggg cggtcgtcgt ctgcggtggg ctcggcgggg tgatggaggc ggcctgccgg
     7201 ggcgcccgcg gcgagggcgg ggtgacggtg ggcctgctcc cgggacccga tcggggggcg
     7261 gcgaacccct acgtgtcggt cgcgatcgcc acggggatgg gcgaggcgcg caacgctctg
     7321 gtcgtgcggg cggcggacgt gctcgtcgcg gtgggcgggt cgtggggcac gctgtcggag
     7381 atcgcgctgg ccatgcgggt cggcaagcgc gtggtggggg tggggacgtg ggagctcgcg
     7441 cgcgcgggcg gcgagccggt ggaggggatc gagcgggcgg cggacgccgc ggaggcggtg
     7501 gggttggcgc tcgggggata ggccgggtcg cggcgcggct ccgtccgccc gcggcggcgc
     7561 tctggtgccg gctccgcccc gcgcctggct ccgcccgcgc ccggcttctc gcccggacgg
     7621 cttcgcctcg cccggcttcg cgcccggcgg ctagctcgcc ggctcggagc caggccggcc
     7681 cggcggggtg gacgacggcg gcgtgagcgt ccgccggtcc tcggcggtga actcgatcgg
     7741 ctcctcgtcc tcccccagcg cacgacgctg cccgcgcgcg gtgcccaccc ggcccccgcc
     7801 ccagatcacg agcgccagga acacgatcgg cagcgcgagg atgggcgccc cgatgctgag
     7861 cgtggcggcg acgattgcgg cgaccagcag gagcatcagc accggtatcg cggctgcggg
     7921 catgcccgag tggattaccc accggcggct gctggcacac tcccgctcca atgacggagc
     7981 gcgagcggcg ccgtgtcgtc gtgcacggtg aggtgcaggg agtcttcttc cgcgactcca
     8041 cgcgggagca ggccgagcgc cgcggtgtgg gcggctgggt gacgaaccgg gacgacggat
     8101 ccgtcgaggc cgtcttcgag ggtgagcccg acgcggtcgc ggcgctcgtc gagttctgcc
     8161 gcgcgggccc atcccaggcg agcgtcgagc gggtggatga gacaagcgag gaacccgagg
     8221 gcctcgaaga ttttcgcgtc gtgtaaccgg cgcgcgtctg aatcgttgcg cttgtaaccc
     8281 acgtgtaacg cgcccacccg tacggtcgcg agcatggagc tcgaccggga gaaactgcgc
     8341 gtcgcggcct ggctcgcggc cgccgtcgtg gtcgcgctgc ttgccgtccg ggcgctcacc
     8401 ggcggcgacg gcggcgcctc agagtccgtg accgtcgacc cggcgggatc ggcggcggag
     8461 agccccggcg cggcggctcc gggtggtggc ggacccggcg cgggggcgtc gggcggcggc
     8521 gtgccgggcg cgcccgtggc cggggcgcct gtattcgtcc acgtggcggg tgaggtccgg
     8581 cgaccggggg tctaccggct gccgcgcggc gcgcgggtca acgatgcggt gcagctcgcc
     8641 ggcggcctca cgcgccgagc ggagcagacg ggcgtgaacc tcgtggcacg ggtctccgac
     8701 gggcagcagg tgatcgtgcc gcgtcgcggc gccgcggcgc ctggcgcgac cggcgcgacg
     8761 cccgggtccg ctggatccgc aggcggcggt gccgcaccgg gggcgaaggt cagcctggcg
     8821 gcggccacac ccgagcagct agacggcatc gacgggatcg gacccacgct ggcgaagcgg
     8881 atcgtggagt accgcgagca gcacggtggc ttccactccg tggacgagct ccgccaggtc
     8941 gacgggatcg gcgagaagcg cttcgagtcc ctgcgcgagg cggtgacccc gtaggtgcgc
     9001 tggcgaccgc cgccgcctgg cttgacacgc gccgtcccca cgtcgcggcg gccgcgctgt
     9061 cggccggctt ggccctctcg ccctggccgg ccacgggctt ctccctggcg gcagcgacct
     9121 ccgccgcgct gctcggtcta gcggcgcgcc tccgcacgct cgcgaccacg agccccgccc
     9181 cgcgcgcggg gaccgctgcg cctatcgccg cttcacccag cgccgctcca cccagcgccg
     9241 ctccacccag cgctgctcca cccagcgctg ctccgccctc gggggccgct ccgcccgcgg
     9301 ccgccccgcc cgcgagcgtc gaccctcccg cgcccacccc gcctgcgggc ttggtgcccc
     9361 cggggatcct tcccgccccc gcggcccacg cggcccccgc ggccaccggg ctcgccgcca
     9421 tcgtcgcggt ggctgcggtc gccgggctcg tcatcgggag cgcccggatc gcgtcgatcg
     9481 accagaccgc ggaacgggcg ctggtcagcg agcgcttcga gggccgcgcg atcctccccc
     9541 aacggccgcg acgcacacgg ttcggatcgt gggcgttcgt cgagctcgcg gccggccccg
     9601 cgaacggctc gcgtgtggtc gcgcgggcgg gtgaggggct tgggtggccg agccacggcg
     9661 agcccggtat cgagctgttc gtggcggggt cgatcgagcg gccgcggcgc acgccggggt
     9721 cgcggctcga ctggcccgcg tatctgaagc gcaggggcgt cgcggccgag ctcgacctcg
     9781 acgcgctccg gcccaccggc ggccggcgcg gtggggtgcg cggctggatt gacgggatcc
     9841 gacgccgcgc ggagcgcgga atcgtcgccg gcgtggcgcc ggaccgagcc gaggtcgccc
     9901 gcgggatggt cctcgggcag gacgagatga tcgatccgct cgtggtcgac gacttccgcc
     9961 gctccgggct cggtcacctc ctcgccgtga gcggccagaa cgtcgtgctg ctctgcgccc
    10021 tcgcgatccc gctgatcgcg gcgctcggtg gcggcatccg cgcgcgcgtc gccgtgctct
    10081 cggtgctcgt gttgctctac gtgctggtcg cgggcgcggg accgtcgctc cagcgtgccg
    10141 cggccatggc ggtcgtcgga ctcgcggcgc tggcggtggg acgtgcccgc tcgcgctggt
    10201 acgcgttgct gctggcggcg gcgatcaccc tggccgtcga cccccgcgcc gccgccgagc
    10261 ccgggtggca gctctcgttc gcggccgtcg ccgggatcct gctcctcgca cccccgctca
    10321 gccgcacgct gcgcgcgctg ccacgcccgc tcgccgacgg cgcggcgatg acgctcgccg
    10381 caacggtcgc caccggcccg atcgcggccc accacttcga ggccgtctcg cttgcgggcc
    10441 tcgtcgcgaa cctggccgcg ctcgcggtcg tggcgccgat cgtctggctt gggatggtcc
    10501 aggcggcgct cggcatcgcg gccggagcgg tacccggcga gctgcccgcc ctcgacgcac
    10561 tggcgatgcg cgccgccgag ctcctcggtc atgtgaacga cgcattcgtc ggcgcgctga
    10621 tccgcatagc gaccgagttc gccgccctcc ccggcgcgca gctgcggctt ccgctggcga
    10681 gcccggtccg cgtgattgcc gcgtacgccg tccccgcgct ggcctggctc gccctgcgcg
    10741 cgctgatccg agcggacgcc gggagctggg ccaagttagg tgtcaaatac gaccaaaaga
    10801 tggcccaggt cgggccgggc ccgcggaccc ggaagggcct ggcgcgggcc gcgcgcgcga
    10861 cccacgccac ccgcgccggc ctcgtcgcag tcgacgccac cgcccgcgcc gccgccgcgg
    10921 cctggcgccg cgcggcgctt ccgaagcgcg ccgccgcagt cgccctcgtc gcggccgggg
    10981 tggcgctcct ctggcagcac gccgctggcc ccgccgcgcc gccggaccgc ctcacggtct
    11041 cgttcctcga cgtcggccag ggcgatgcca cgctgatcca ggacggtcac ggcgccgcgg
    11101 tcctcttcga cggcggtccg cccgaggcgc gcacgtaccg gctgctccgc cgggcggggg
    11161 tccgccgcct ggccctcgtg gtcgcgactc acgcgtcgcg cgaccaccac ggcggcctcg
    11221 ccgaggtcct ccgccgcatc cccacccagg cactactcga cggcggcgac ggaacgcgcg
    11281 accccagctt cgcgcagatg ctcgaggagg ccaggcgtcg cggcgtccgc caca
//
LOCUS       DASVPL010000145         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_212670, whole genome shotgun sequence.
ACCESSION   DASVPL010000145 DASVPL010000000
VERSION     DASVPL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212670"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1049
                     /locus_tag="VF517_07485"
     CDS             174..1049
                     /locus_tag="VF517_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX8052818.1"
                     /translation="MLAAAALLPSAAVPAPAAATEVTRTELSDLAERARTDIAALHEL
                     RRVDRVDGKPYAIEDALAGARDERLDERLEEIAALSGGGAGEERSAPLGGARGDAQDI
                     LSDNRYEGSDVPRPFKGVLDKIGDFLEPIGEWIENAFDDLASGLPGGDVTLWSLTAAL
                     ILIALLTLGSRTLRARAQEGAEARAAAAGPDERDTPGRLERAADEAERAGDLETALRL
                     RFRAGLLRLDARDAIRFRPSISTREVSHALNSPEFDRLAALFDGVAYGGEPASRDDLA
                     ASREGWDAVLKGAAR"
     gene            1072..2181
                     /locus_tag="VF517_07490"
     CDS             1072..2181
                     /locus_tag="VF517_07490"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX8052819.1"
                     /translation="MRQRFRALPGGWRAALVFVAIAAGLNLIGALGNRLAPEPSGPPY
                     SSYSTSADGAAGLAELLERQGRAVEQLRGDLSGTEIDPDATVFVLGADRLAASEMRVL
                     RSFLERGGRLVGGGPDPHWLSGIADVTGMWSEGGSRELRPLVPAPEVRTVDEVRADGF
                     GTWRVDAPGLPILGGESGSVLLSKRVGAGRALLMADVSPLQNRSLDEGDNATLALALA
                     GERSRKVIFVEGVHGFGESRGLAALPDDWKWALIGLGLAGLAGIWAIGRRLGPPEDER
                     RPLPPPRRRFVDAQALTLARTRDPAAAVTPVRDAARERLTRRAALGPEAGEPQLRRAA
                     EHLGLQPDETDAILGRNSDPLAAGRALARLSGGRT"
     gene            2178..3107
                     /locus_tag="VF517_07495"
     CDS             2178..3107
                     /locus_tag="VF517_07495"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8052820.1"
                     /translation="MRELHGRLAGEVRKVVVGQERVLELLTCTLAVGGHALLQGVPGT
                     AKTLLANSLARALSVEFRRVQFTPDMLPSDLTGTMTLRGGELGFRQGPVFTNLLLADE
                     INRTPPKTQAALLEAMQERQVSVDGEPHPLPDPFLVVATQNPIEYEGTYPLPEAQLDR
                     FLVQIEVGYPGADDELAMVQRPRRGVEAGGLDQVQPVATPVDLRAVREEVDATEVAPE
                     VARYVVELVRRTRELPSVTLGASPRAAVHLLAASKACARLYGRAFVTPDDVGYMAGPV
                     LRHRLVLTPEAELERYGPDEAVNTALGAVPVPR"
     gene            3107..>4291
                     /locus_tag="VF517_07500"
     CDS             3107..>4291
                     /locus_tag="VF517_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8052821.1"
                     /translation="MAPTPRAALVLAAAALSALFVPLWVAGLLGVAVVGAVVADALAV
                     RRAPRVERSHPAVLARGVPAPLELASDGGAGGRARIRQPVPPDVRLEPREADERLDAT
                     LTPIRRGRHPLPAPALRAHGPLGLGAWYHRPGEEGELLVYPDLPSARRIALAVARGRF
                     LEGGQRGRGPLGLGTDFESIRDYLPDDDVRQVNWRATARMGRPMSNQYRVDRDRELMC
                     LVDCGRLMAAPLGDLTRLDAAVDAVTALALVADELGDRIGAVAFDDEVRHHLRPRRDG
                     GRAVVQRLFDVEPASVESDYELAFRIAEGAKRSFVLVLTDLLEPAAAESLVEAVPVLS
                     RRHEVVVASVTDVDLTSAIATPPERELDVYAAAAAVEVLGAQAEAATLVRRAGARVIE
                     APP"
BASE COUNT          486 a         1650 c         1624 g          531 t
ORIGIN      
        1 ccgcccgcgc gacagtggga cccgccacag ccccgatggg acccgcctgc gccgcctccg
       61 ccacccgacc cctccgaccc cgcgccgagc tgagcccgcg cgcattcatc cacgaggtgt
      121 tcggattttg tggcggtatg cgccacttag gccgagcacc tcgtctgggt gcggtgctgg
      181 ccgccgccgc gctgctcccc agtgccgccg ttccggctcc cgccgcggcc accgaggtca
      241 cccggacgga gctctccgac ctcgcggagc gcgcgcgcac ggacatcgcc gcgctgcacg
      301 agctgcgtcg ggtcgaccgg gtggacggga agccgtatgc catcgaggac gccctcgccg
      361 gtgcgcgcga cgagcggctc gacgagcgcc tcgaggagat cgccgcgctc agcggtggcg
      421 gcgcggggga ggagaggagc gccccgctgg gcggcgcgcg cggcgacgcc caggacatcc
      481 tctccgacaa ccgctacgag ggcagcgacg tgccgcgtcc gttcaagggc gtgctcgaca
      541 agatcgggga cttcctcgaa ccgatcggcg aatggatcga gaacgcgttc gacgacctcg
      601 cgtccggcct gccgggcggc gacgtgacgc tctggagcct caccgccgcg ctgatcctga
      661 tcgcgctgct caccctcggc tcgcgcaccc tgcgcgcccg tgcgcaggag ggcgccgagg
      721 cccgtgccgc cgccgcgggc cccgacgagc gcgacacccc cgggcgactg gagcgggcgg
      781 cggacgaggc cgagcgggcg ggcgacctcg agaccgcctt gcggctgcgc ttccgcgccg
      841 gcctgctgcg cctcgacgcc cgcgacgcga tccgcttccg cccctcgatc tccacgcgcg
      901 aggtctcaca cgcgctcaac tcgcccgagt tcgaccggct cgcggcgctg ttcgacggcg
      961 tggcctacgg gggcgagccc gcctctcggg acgacctcgc cgcctcgcgc gagggctggg
     1021 acgcggtgct gaagggcgcc gcgcggtgat gaagcgcttc ggggccgccc gatgaggcag
     1081 cgcttccgag ccctaccggg cggctggcgc gccgcgctcg tcttcgtcgc catcgcggcc
     1141 gggctcaacc tgatcggcgc gctcggcaac cggctcgcgc ccgagccgag cggcccgccc
     1201 tacagctcgt actcgaccag cgccgatggg gccgccggcc tcgccgagct gctcgagcgc
     1261 cagggccgcg cggtcgagca actgcgcggc gacctgtccg gcaccgagat cgacccggac
     1321 gcgaccgtct tcgtgctcgg ggctgaccgg ctggccgcca gcgagatgcg cgtgctgcgc
     1381 tcattcctgg aacgcggcgg gcgactggtc ggcggcgggc ccgacccgca ctggctgagc
     1441 gggatcgcgg acgtgaccgg gatgtggtcg gagggcgggt ccagggagct gcggcccctc
     1501 gttcccgcgc cggaggtgcg cacggtcgac gaggtacgcg ccgacggctt cggcacctgg
     1561 cgggtcgatg cgcccggcct gccgatcctc ggcggcgaga gcggcagcgt gctcctctcc
     1621 aagcgggtcg gggccggccg cgccctcctg atggcggacg tctcgccgct ccagaaccgc
     1681 tcgctcgacg agggcgacaa cgcgacgctc gccctcgccc tcgcggggga gcgctcgcga
     1741 aaggtgatat tcgtcgaggg cgtgcacggg ttcggggagt cgcgcgggct tgcggccctc
     1801 ccagacgact ggaagtgggc gctgatcggg ctcgggctgg cggggctcgc cgggatctgg
     1861 gcgatcgggc gccggcttgg cccaccggag gacgagcgcc ggccgctccc gccgccgcgg
     1921 cggcggttcg tggacgctca ggcgctgacg ctcgctcgca cccgcgatcc cgccgcggcc
     1981 gtgacgccgg tccgcgacgc cgcgcgcgag cggctcaccc gacgcgccgc gctcggcccc
     2041 gaggccggtg agccgcagct gcgccgcgcg gcggagcacc tcggcctcca gccggacgag
     2101 accgacgcga tcctcgggcg caacagcgac ccgctcgccg ccggccgtgc gctcgcgcgc
     2161 ctcagcggag gccgcacgtg agggagctcc atggccggct cgccggcgag gtgcggaagg
     2221 tcgtggtcgg ccaggagcgc gtgctcgagc tcctcacctg cacgctcgcc gtcggcggtc
     2281 acgcgctgct ccagggcgtg ccgggcacgg cgaagaccct cctcgcgaac tcgctcgccc
     2341 gcgcgctgtc ggtcgagttc cggcgggtcc agttcacccc ggacatgctc ccgtcggacc
     2401 tcacggggac gatgacgctg cgcggcggcg agctcggctt ccggcagggc ccggtcttca
     2461 cgaacctgct cctggcggac gagatcaacc gcacgccgcc gaagacccag gcggccctgc
     2521 tcgaggcgat gcaggagcgg caggtgtcgg tcgacggcga gccgcacccg ctgcccgacc
     2581 cgttcctcgt cgtcgccacc cagaacccga tcgagtacga ggggacctac ccgctgccgg
     2641 aggcccagct cgatcgcttc ctggtgcaga tcgaggtcgg ctacccgggc gccgacgacg
     2701 agctggccat ggtgcagcgc ccgcggcgcg gagtcgaggc gggcgggctc gaccaggtgc
     2761 agccggtggc caccccggtc gacctgcgtg cggtgcgcga ggaggtcgac gccaccgagg
     2821 tcgcgccgga ggtcgcgcgc tacgtggtcg agctggtgcg ccgcacgcgc gagctgccga
     2881 gcgtgacgct cggcgcgagc ccgcgcgcgg ccgtgcacct gctggccgcg tcgaaggcgt
     2941 gcgcgcggct ctacggccgg gccttcgtga cccccgacga cgtcggttac atggcggggc
     3001 cggtgctccg ccaccggctc gtgctcacgc ccgaggccga gctcgagcgc tacggccccg
     3061 acgaggcggt gaacaccgcc ctcggcgccg tcccggtccc gcgctgatgg ctcccacgcc
     3121 gcgcgcggcc ctcgtgctcg cggcggccgc gctcagcgcc ctgttcgtgc cgctgtgggt
     3181 ggccgggctg ctcggcgtgg ccgtggtcgg cgccgtggtg gccgacgcgc tggccgtgcg
     3241 ccgggcgccg cgcgtcgagc gctcgcatcc cgcggtgctg gcgcgggggg tgcccgctcc
     3301 gctcgaactt gcgtcggacg gcggcgcggg tggccgggcc cgcatccgcc agccggtccc
     3361 gcccgacgtc cgcctcgagc cgcgcgaggc cgacgagcgc ctcgacgcga cgctcacccc
     3421 gatccgtcgc ggccgccacc cgctgcccgc gcccgcgctg cgcgcgcacg gcccgctcgg
     3481 actcggcgcc tggtaccacc ggccggggga ggagggcgag ctgctggtct accccgacct
     3541 gccgtcggcg cggcggatcg cactcgccgt cgcccgcggg cgcttcctcg agggcggtca
     3601 gcgcgggcgc gggccgctcg gcctcgggac cgacttcgag tcgatccgcg actacctgcc
     3661 cgacgacgac gtccgccagg tcaactggcg cgcgacggcc cgcatggggc gcccgatgag
     3721 caaccagtac cgcgtggacc gcgaccgcga gctgatgtgc ctggtcgact gcgggcgact
     3781 gatggcggcg ccgctgggcg atctgacccg gctggacgcg gccgtcgacg ccgtgaccgc
     3841 gctcgccctc gtcgccgacg agctgggcga ccggatcgga gcggtggcat ttgacgacga
     3901 ggtgcgccac cacctccgcc cgcgccgcga cggcggccgc gccgtggtgc agcggctctt
     3961 cgacgtggag cccgcgtccg tggagagcga ctacgagctg gcgttccgga tcgccgaggg
     4021 agccaagcgc tcgttcgttc tcgtgctcac cgacctgctc gaaccggccg cggcggagtc
     4081 gctcgtcgag gcggtgccgg tgctgtcgcg ccgccacgag gtcgtggtcg cctcggtgac
     4141 ggacgtggac ctgaccagcg cgatcgcgac cccgccggag cgcgagctcg acgtctacgc
     4201 cgccgccgcg gcggtcgagg tgctcggcgc gcaggccgag gccgccacgc tcgtgcgccg
     4261 cgcgggcgcg cgggtgatcg aggcgccgcc g
//
LOCUS       DASVPL010000146        12583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_134120, whole genome shotgun sequence.
ACCESSION   DASVPL010000146 DASVPL010000000
VERSION     DASVPL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12583
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134120"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF517_07505"
     CDS             <1..232
                     /locus_tag="VF517_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052822.1"
                     /translation="LRRFGEDAPPAGGLPRAELPARLFRQLERTRAAVRKYGTRGAQR
                     RLRISRRRMLQRLSLDRRLRALGPFGRLSCGR"
     gene            complement(229..522)
                     /locus_tag="VF517_07510"
     CDS             complement(229..522)
                     /locus_tag="VF517_07510"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX8052823.1"
                     /translation="MDLASERAREVLRRVRAVPEGFVTTYGELSPGAPRFAGSVLSAC
                     RDPDVPWQRVVRADGSLAKGERQRVLLEDEGVPFAGARVDMDRAWIPAGDLVG"
     gene            complement(530..2116)
                     /gene="aceB"
                     /locus_tag="VF517_07515"
     CDS             complement(530..2116)
                     /gene="aceB"
                     /locus_tag="VF517_07515"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX8052824.1"
                     /translation="MSGVEVHAPVEGRFAEVLTEDALAFVADLHRRFDPRRRELLAAR
                     AERVARWNAGALPDFLTDTRAVREGDWTVAAPPPDLQDRRVEITGPTDRKMVINALNS
                     GARGFMADFEDANSPTWSNMIGGHVNLTDAIEGTIEHEENGKSYSLGEERATLLVRPR
                     GWHLTDRHITVDGAPVSGSLMDFGLHVFHNARRLLDHGSGPYFYLPKLESHLEARLWN
                     EVFVAAQEALGLDRGTIRATVLIETVPAAFEMEEILYELREHSAGLNAGRWDYIFSMI
                     KCFRDRPEFLLPDRVSVTMTVPFMRAYTLLLVRTCHRRGAHAMGGMSAVIPSRRDAEA
                     NERAFAAVRSDKEREAGDGFDGSWVAHPDSVPVAMERFDEVLGERPNQVERRREDVIV
                     AASDLLDAGSAGGEITEAGLRGNVNVGIQYISSWLRGNGAAAINHLMEDAATAEIARS
                     QVWQWIRHGAEVDGQRVTPELVRRIETEELERIREQVGDEFFRTQGRPEESRELFEDV
                     ALSDEFVDFLTLPAYERLED"
     gene            complement(2113..3396)
                     /locus_tag="VF517_07520"
     CDS             complement(2113..3396)
                     /locus_tag="VF517_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX8052825.1"
                     /translation="MSTAFDSVRPPSADTIADCVHCGFCLPTCPTYSLWGEEMDSPRG
                     RIVLMKQGLEEESELSRTLVTHIDRCLGCMACVTACPSGVRYDELINDSRAQIERNFE
                     RGPRERLTRWLTFEIATYPRRLRALAPFAAAAHALGLPSLARRRRGGLLGRVPRLRTA
                     LELTPEAGLRTESPPETTAAIGTRRGRAGLLQGCVQRAYFGAVNAASARVLAAEGWEV
                     VARPQPRCCGSLQLHTGDDEPARALARETIDAFADCDVVVANVAGCGSGMKDYGHLLR
                     DDEAWAGRAAAFSAKVRDVNELLAEHEPRAPRGRVDLRVAYHDACHLAHAQAVRAQPR
                     ELLRGIPGVELVEPAEWEICCGSAGIYNIVNPEPAADLGRRKARNLLATGVDTVAAAN
                     PGCALQIAAHAERLGKPLRVVHPVEILDESLRAAT"
     gene            complement(3396..4484)
                     /locus_tag="VF517_07525"
     CDS             complement(3396..4484)
                     /locus_tag="VF517_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8052826.1"
                     /translation="MYDAVAAALREASQNARRVRVRGGGTKLGWGAAAPAPDVEIDMR
                     EHDRILEHNAGDLTAVVQAGVPLARLQDELAAHGQMLAIDPPRSDAATLGGTIATGDS
                     GPLRHRYGAPRDLVLGMTVALSDGTIARTGGKVIKNVAGYDLAKLFTGSFGTLGAILD
                     VSLRLHPVPPTATAVGAAGDPGRVAAAATALAHSAIEAQCLDVRWGRGDGHVLVRVAG
                     GEAPAQAAEAARLLAGTGLETTVEEDDAALWEDQRAWQRSADGVVARVSGVQDQLADQ
                     MRVAERLGGSLVARAAHGLAWVTLPDAGAVDALRRELTPSPCVVLDGDVADPWGVANS
                     GAIELMRRVKQRFDPAGACSPGVFVGGI"
     gene            complement(4501..5946)
                     /locus_tag="VF517_07530"
     CDS             complement(4501..5946)
                     /locus_tag="VF517_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8052827.1"
                     /translation="MPDLVAELEGICGGGNVVKHPHALRTYESDGLLQYAVMPRVAVL
                     PDTAAQVRDVVRACHRAGVPWVARGAGSGLSGGALPIEEGVLIVLTRLRRIVEVDLAN
                     QRVVAEPGVTNLAISHAVAPSHFFPPDPSSQIVCSIGGNVAENSGGAHCFKYGFTTNY
                     VTGLEVVLADGAVVELGGKGVDLPGYDLVGAFVGSEGTLGVATRVTLRVVPRPQAVRT
                     LVAFFASTAEAGQTVSDIVSAGLIPGALEMMDRLSIEAAEGATNAGYPTDAEAALIVE
                     LDGSETECEARFDEVVALCRTNGATGVRVAADEAERELIWKARKAAFAAMGRLSPNYY
                     VQDGVIPRTRLPEVLARIDELSGDAGLRVANVFHAGDGNLHPLVLYDAAVAGEAERAE
                     ELAGAIVTACVDAGGSITGEHGVGIDKKRYMPRQFAEADLDAFQRLRCAFDPAGLANP
                     GKVMPTPRLCGEVPGPYRQHPLEAAGIAERF"
     gene            5971..6777
                     /locus_tag="VF517_07535"
     CDS             5971..6777
                     /locus_tag="VF517_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8052828.1"
                     /translation="MPIAEANGQRLYYEVHGEGEPLLCVMGLGGDHTAWILQVAEWSK
                     TRRVVVFDNRDVGQSSYADGPYEVSDMAADALALADVLGLLSFDLVGISLGGAIAQEI
                     ALAAPGRVRTATIGVSYAASGNWGAHRAKVLGGIVRGMTREERVELVLMLVYSEIVFA
                     NPNFTDAARAAMLGNPHPQEPEAFIRQLEAGSRHDARSRLGELSLPVQVIGAMRDVMI
                     PPQASPELAGLIPGARLTMLECGHLVNVEAAQEFNRLVLDFAAEHAGAPA"
     gene            6824..7684
                     /locus_tag="VF517_07540"
     CDS             6824..7684
                     /locus_tag="VF517_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052829.1"
                     /translation="MPRLALILVALLAAAVLVPGAASATPMQESASAPSPGGEVAAQL
                     TYDKQDEFQYSNVRIKITRGGAVLLDAAVPAPCDECPTSPQGGGDPEIPSLVVRDLNG
                     DGEPEALVDLYTGGAHCCSYTQIYGYHAATNAYRRVKATWGDFGYQLTDLNKDGKPEF
                     RSSDFRFAGAFTAYAASGAPPMILNYTGGKLVNNTRKFRREIKKNLKQYVALYKEIRK
                     DPDVPDVRGFLAAYVADKYLLGQGDTAFDLVYAALRRGELKALEGDTSPAGKKYISAL
                     RKYLRKWGYR"
     gene            complement(7681..8496)
                     /locus_tag="VF517_07545"
     CDS             complement(7681..8496)
                     /locus_tag="VF517_07545"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX8052830.1"
                     /translation="MAIRCVYTDLDGTLLGRGASLFRDHEGAFTLLPARALEACHRCD
                     VEVVLKSGRRKAQVMEDARLLGQSAYIYEVGCGLHLDGDDSYLTGELQPTADRTVYAQ
                     IDESGAPALLLERYAGRFEYHDPWHEGREFSHIFRGLVDTTEADALLASSGHGQLRLV
                     DNGSISPRETLQDLPGPPHCYHLIPRTASKASAVATHMRARGYAREECIAVGDSREDV
                     GVAEVVGRFFLVANAVEKDPSIATAGGANVEVTEAAAGEGFYEAVVRSLAEAR"
     gene            8523..10793
                     /locus_tag="VF517_07550"
     CDS             8523..10793
                     /locus_tag="VF517_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8052831.1"
                     /translation="MAVTRTRAPWEGLIESGRDEQIVYEGRLAARPAARVPVPDHLHP
                     ELLDALERDGVTELYTHQADALSSALEGDTIVTTGTASGKSLAFNLPVLDTLARDRRA
                     RAFYLYPTKALAQDQARKLTGLKAPFLKHAIYDGDTPQSERRSIRARSNLILTNPDML
                     NVGVLPHHRQWGDVLANLAWIVVDEAHVYRGVFGSHVANVLRRLRRLARAYGTEPRFV
                     LASATIANPGELAEKLTGKECRLVDRDGAPRHERKIAMWNPPLTDEATGKRVSVLSEA
                     AGVLAKLVEDGVRTICFMRSRRGVELVQNFTRLRLEDMGRADLAERIAPYRAGYTPTQ
                     RRDIERRLMEGDLLAVVATNALELGIDVGDLEASICITYPGTVASLRQMWGRAGRREA
                     GLAMYMAGEDALDQFFCRHPEDFLSRPIESAILDHENESIHLPHLVAAAYELPLTGED
                     REILGPRWEAYADRLVRIGHLRERDGRYLPRGAEFPAGRISLRSASIDSIAVVEEGRG
                     EVIGTVEGERAHSTVHPGAVYMHLGQSYEVEELDLRGRSALVRSFSGDWYTQPKKETE
                     TFIEEVRRQRMVGTVELNFGMVSVSEEVVAFQKKRVSDHEVLDLLPVELPMQRFVTQA
                     LWYVMPEGLFADDFPLEVLQGSLHAAEHAQIAVLPLIAMCDRWDIGGLSTAYHHQTGR
                     PTIFIYDGHPGGVGITRAGFEAFERLCDDALLLISECPCKSGCPSCVQSPKCGNLNDP
                     LDKHGSVELLQRIRAA"
     gene            10843..>12583
                     /locus_tag="VF517_07555"
     CDS             10843..>12583
                     /locus_tag="VF517_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8052832.1"
                     /translation="MEATATALFEYLRRADARQHARALDLRERITAWLEYIPATFPHY
                     TRHTVAHSDEIVRQLSALLFASSDPSKPTVQLSSIEAYVLLAAAYLHDAGMVVSNNAK
                     MDVLSSSEWESWTSGGNPGADRWQAILALRAEAEEDSSPHLHFAADLETRFLLADYFR
                     RTHASRAAQLIQTYRLHLGLADADPTLVETISAVCVAHGASIHDLEDSVRYPERRMTA
                     GEQVNVRFLALLLRLGDLLDMSTDRSCSLLVNAAAPLPADSLAHWTQYSSIVHRLTAP
                     DRIEVRAKCHDHEEHRVLRDWCDWLAQEVTNAAALMAHSARHGDWRAPITRSRGPDPT
                     IEIDRAQDATYVLEDWKLEIDADAVLTRLTEDIYGEQGAFLRELIQNALDASRCQMYL
                     DLRAEGIEPPPNPAHVEAAFRDKYGVRVAIDEILTESPVSGKRERRHVITVEDSGIGM
                     DREVIQRFFLQIGRSFYTSREFDRVFTFVPTSRFGLGFLSVFGVSDDVTVETYKPSSA
                     AQDGPLRMRLTGSKSYLITEEGNRTTSGTTITVVLRDLRPARFVLDTVRMWCRMVELP
                     IEVRSFGEAECVTA"
BASE COUNT         1839 a         4492 c         4449 g         1803 t
ORIGIN      
        1 gctgcggcgc ttcggcgagg acgcgccccc ggctggcggc ctgccacgcg ccgagctgcc
       61 ggcgcggctc ttccggcagc tcgagcgaac acgcgccgcg gtccgcaagt acggcacccg
      121 cggcgcgcag cggcgcctgc ggatctcgcg caggcggatg ctccagcgcc tgtcgctcga
      181 ccgcaggctg cgagcgctcg gcccgtttgg ccggctctcc tgcggccgct agcccacgag
      241 gtcgccggcg ggaatccagg cgcggtccat gtccacccgc gcgcccgcga acggcacgcc
      301 ctcgtcctcg agcagcacgc gctggcgctc gcccttggcg agcgaaccgt ccgcccgcac
      361 gacccgctgc cacggcacgt ccgggtcccg gcacgcgctc agcacgctcc cggcgaaccg
      421 gggcgccccg gggctcagct cgccgtaggt ggtcacgaac ccctcgggca ccgcgcgcac
      481 ccgccgcagg acctcacgcg cgcgctcgct cgcgaggtcc acgccgcgct cagtcctcca
      541 gccgctcgta cgccggcagc gtcaggaagt ccacgaattc gtcgctcagc gcgacgtcct
      601 cgaacagctc gcgcgattcc tccggccggc cctgcgtgcg gaagaactcg tcgcccacct
      661 gctcgcggat ccgctcgagc tcctcggtct cgatccggcg cacgagctcc ggcgtgacgc
      721 gctggccgtc gacctcggcg ccgtggcgga tccactgcca cacctgcgag cgggcgatct
      781 ccgccgtcgc ggcgtcctcc atcaggtggt tgatcgccgc ggcgccgttg ccgcgcagcc
      841 aggacgagat gtactggatg cccacgttca cgttcccgcg cagcccggcc tcggtgatct
      901 cgccgccggc ggagcccgcg tcgagcaggt cggacgcggc gacgatcacg tcctcgcgcc
      961 ggcgctcgac ctggttcggc cgctcgccga gcacctcgtc gaagcgctcc atggccaccg
     1021 gtaccgagtc ggggtgcgcg acccagctgc cgtcgaaccc gtcaccggcc tcgcgctcct
     1081 tgtcggagcg gacggcggcg aacgcccgct cgttcgcttc ggcgtcgcgc cggctcggga
     1141 tcaccgcgga catgccgccc atcgcgtgcg cgccgcggcg gtggcaggtg cgcacgagca
     1201 gcagcgtgta ggcgcgcatg aacggcaccg tcatagtcac gctcacgcgg tccggcagca
     1261 ggaactcagg ccggtcgcgg aagcacttga tcatgctgaa gatgtagtcc cagcggccgg
     1321 cgttgagacc ggccgagtgc tcgcgcagct cgtagaggat ctcctccatc tcgaacgcgg
     1381 ccgggaccgt ctcgatcagc acggtcgcgc ggatcgtgcc gcggtcgagg ccgagcgcct
     1441 cctgagccgc gacgaagacc tcgttccaga gccgcgcctc gagatggctc tcgagcttgg
     1501 gcaggtagaa gtacggcccg gacccgtggt cgagcaggcg ccgcgcgttg tggaagacgt
     1561 gcaggccgaa gtccatgagg ctgcccgaga cgggcgcgcc gtcgacggtg atgtggcggt
     1621 cggtgaggtg ccagccgcgc ggccgcacga gcagcgtggc gcgctcctcg ccgaggctgt
     1681 aggacttgcc gttctcctcg tgctcgatcg tgccctcgat cgcgtcggtg aggttcacgt
     1741 gcccgccgat catgttcgac cacgtgggcg agttcgcgtc ctcgaagtcg gccatgaacc
     1801 cgcgggcgcc ggagttgagc gcgttgatga ccatcttgcg gtcggtcggg ccggtgatct
     1861 ccacgcggcg gtcctggagg tcgggcgggg gcgcggccac ggtccagtcg ccctcgcgta
     1921 ccgcgcgcgt atcggtgagg aagtcgggaa gcgcgcccgc gttccagcgc gcgacgcgct
     1981 cggcacgcgc ggcgagcagc tcgcgccggc gcgggtcgaa gcggcggtgc aggtccgcga
     2041 cgaacgcgag cgcgtcctcg gtcaggacct cggcgaagcg gccctcgacg ggcgcgtgga
     2101 cctcgacgcc gctcacgtgg ccgcccgcag cgactcgtcg aggatctcga cgggatgcac
     2161 gacgcgcagc ggcttgccga ggcgctcggc gtgcgcggcg atctggagcg cgcagccggg
     2221 gttcgccgcc gccacagtgt cgactccggt ggcgagcagg ttgcgcgcct tgcgccgccc
     2281 gaggtcggcc gccggctcgg ggttgacgat gttgtagatc ccggccgagc cgcagcagat
     2341 ctcccactcg gcgggctcga ccaactccac ccccggaatg ccgcgcagca gctcacgcgg
     2401 ctgggcgcgc acggcctggg cgtgcgccag gtggcaggcg tcgtggtagg ccacacggag
     2461 gtccacgcgc ccgcgcggcg cgcgtggttc gtgctcggcc agcagctcgt tcacgtcgcg
     2521 taccttcgcg gagaatgccg cggcgcgtcc ggcccaggcc tcgtcgtcgc gcagcaggtg
     2581 gccgtagtcc ttcatcccgg agccgcagcc ggccacgttg gcgaccacca cgtcgcagtc
     2641 ggcgaacgcg tcgatcgtct cgcgcgcgag tgcgcgggcc ggctcgtcgt cgccggtatg
     2701 aagctggagc gagccgcagc agcgcggctg cggccgggcc accacctccc agccctcggc
     2761 ggcgagcacg cgtgcgctgg cggcgttcac cgcgccgaag taggcgcgct gcacgcagcc
     2821 ctggagcagg cccgcccggc cgcggcgggt gccgatggcg gcggtggtct ccggcgggct
     2881 ctccgtgcgc agcccggcct cgggggtcag ctcgagcgcg gtgcgcagcc gcggcacgcg
     2941 gccgagcagc ccgccgcgac ggcgccgggc gagcgacgga aggccgagcg cgtgcgcggc
     3001 cgccgcgaac ggcgcgagcg cgcgcaggcg gcgcggatag gtggcgatct cgaacgtgag
     3061 ccagcgggtg aggcgctcgc gcgggccgcg ctcgaagttg cgctcgatct gcgcgcgcga
     3121 gtcgttgatc agctcgtcgt agcggacccc ggacgggcag gccgtcacgc aggccatgca
     3181 gccgaggcag cggtcgatgt gggtcacgag cgtgcgcgac agctcgctct cctcctcgag
     3241 cccctgcttc atcagcacga tgcgcccgcg cggcgagtcc atctcctcgc cccagagcga
     3301 gtacgtcggg cacgtgggga ggcagaagcc gcagtgcacg cagtcggcga tggtgtccgc
     3361 cgagggcggg cgcacgctgt cgaacgcggt gctcatcaga tgcctcccac gaacacgccg
     3421 gggctgcacg cgcccgcggg gtcgaagcgc tgcttcacgc ggcgcatgag ctcgatcgcc
     3481 ccgctgttgg cgacccccca cgggtccgcc acgtcgccgt cgagcaccac gcacggcgat
     3541 ggcgtgagct cgcgccgcag cgcgtccacc gcgccggcgt cggggagggt gacccaagcg
     3601 aggccgtgcg ccgcccgggc gacgagcgag ccgcccaggc gctcggccac gcgcatctgg
     3661 tcggcgagct ggtcctggac ccccgacacg cgcgccacga cgccgtcggc cgagcgctgc
     3721 cacgcgcgct ggtcctccca gagcgccgcg tcgtcctcct cgaccgtggt ctcgagtccc
     3781 gtgcccgcca ggagccgcgc cgcctccgcg gcctgcgccg gtgcctcgcc gccggccacc
     3841 cgcacgagca cgtgcccgtc gccgcggccc cagcggacgt cgaggcactg ggcctcgatc
     3901 gccgagtgcg cgagcgccgt cgccgcggcg gccactcgac cggggtcgcc cgccgcgcca
     3961 accgcggtgg cggtgggggg gacgggatgc agccgcaggg acacgtcgag gatcgcgccg
     4021 agcgtgccga acgagccggt gaacagcttc gcgaggtcgt agcccgccac gttcttgatc
     4081 accttgccgc ccgtccgggc gatcgtgccg tcgctcagcg cgacggtcat gccgagcacc
     4141 aggtcgcgcg gtgctccgta gcggtggcgg agcgggccgc tgtcaccggt ggcgatcgtg
     4201 ccgccgaggg tcgcggcgtc cgaacgcggt ggatcgatcg cgagcatctg cccgtgcgcg
     4261 gccagctcgt cctgcagacg cgcgagcggc acgcccgcct gcacgaccgc ggtgaggtct
     4321 ccggcgttgt gctcgaggat ccggtcgtgc tcgcgcatgt cgatctcgac gtccggcgcc
     4381 ggagccgcgg ctccccagcc gagcttggtc ccaccgccgc gcacgcgcac gcgccgggcg
     4441 ttctgcgaag cctcgcggag ggcggccgcg acggcgtcgt acatcagacg tcctcaccca
     4501 tcagaaccgc tccgcgatcc ccgcggcctc gagcgggtgc tggcggtacg gaccgggcac
     4561 ctcgccgcac agccgcggcg ttggcatgac cttgccgggg ttggcgagcc cggcggggtc
     4621 gaacgcgcag cgcagccgct ggaacgcgtc gaggtcggcc tcggcgaact ggcgcggcat
     4681 gtagcgcttc ttgtcgatgc ccaccccgtg ctcgcccgtg atcgagccgc ccgcgtccac
     4741 gcacgccgtg acgatcgcgc ccgccagctc ctcggcgcgc tcggcctcgc ccgccaccgc
     4801 tgcgtcgtag agcaccagcg ggtgcaggtt gccgtcgccc gcgtggaaga cgttggccac
     4861 gcgcagcccg gcgtcgccgg acagctcgtc gatgcgcgcg agcacctcgg gcagccgcgt
     4921 gcgcgggatc accccgtcct gcacgtagta gttcggcgag agccggccca tggcggcgaa
     4981 cgccgccttg cgcgccttcc agatcagctc gcgctcggcc tcgtcggcag ccacgcgcac
     5041 gccggtggcg ccgttcgtgc ggcagagggc gaccacctcg tcgaagcgcg cctcgcactc
     5101 ggtctcggag ccgtcgagct cgacgatcag ggcggcctcc gcgtcggtcg ggtagccggc
     5161 gttcgtggcg ccctcggcgg cctcgatcga gagccggtcc atcatctcca gagcaccggg
     5221 gatgagtccg gcggagacga tgtcggagac ggtctggccc gcctccgccg tcgaggcgaa
     5281 gaacgcgacg agcgtgcgca cggcctgcgg ccgcggcacc acgcgcaggg tcacgcgcgt
     5341 ggccacgccg agcgtcccct cggatccgac gaacgcgccc acgaggtcgt agcccggcag
     5401 gtccactccc ttgccgccca gctccaccac cgcgccgtcc gccagcacga cctcgaggcc
     5461 ggtcacgtag ttcgtggtga agccgtactt gaagcagtgc gcgccgccgg agttctcggc
     5521 cacgttgccg ccgatcgagc agacgatctg gctcgacggg tcgggcggga agaagtgcga
     5581 cggcgcgacg gcgtgcgaga tggccaggtt cgtcacgccc ggctcggcca ccacgcgctg
     5641 gttcgccagg tcgacctcga cgatccgccg caggcgcgtg agcacgatca gcacgccctc
     5701 ctcgatcggg agcgcgccgc cggacagccc ggagcccgcg ccgcgcgcca cccacggcac
     5761 gccggcgcgg tgacaggcgc gcaccacgtc gcgaacctgc gccgccgtgt cgggcaggac
     5821 cgccacgcgc ggcatcacgg cgtactggag cagcccgtcg gactcgtacg tgcgcagggc
     5881 gtgcgggtgc ttgacgacgt tgccgccgcc gcagatcccc tcgagctccg ccaccaggtc
     5941 cggcacgccg gagacggtac cctgccgctc gtgccgatcg cggaggcgaa cgggcagcgg
     6001 ctctactacg aggtccacgg cgagggcgag ccgctgctgt gcgtgatggg gttgggcggc
     6061 gaccacaccg cctggatcct ccaggtcgcg gagtggtcga agacgcggcg cgtggtggtg
     6121 ttcgacaacc gcgacgtggg gcagtcctcg tacgccgacg ggccctacga ggtgagcgac
     6181 atggcggccg acgcgctggc gctcgcggac gtgctcggcc tcctctcgtt cgacctggtc
     6241 ggcatctccc tcggcggggc catcgcacag gagatcgcgc tcgcggcgcc cggccgggtg
     6301 cgcaccgcca cgatcggcgt gagctacgcg gccagcggca actggggcgc gcaccgcgcg
     6361 aaggtgctcg gggggatcgt gcgcgggatg acgcgcgagg agcgcgtcga gctggtgctg
     6421 atgctcgtct actcggagat cgtgttcgcg aacccaaact tcacggacgc ggcgcgggcg
     6481 gcgatgctcg gcaacccgca tccgcaggag ccggaggcct tcatacgcca gctcgaggcc
     6541 gggtcgcggc acgacgcgcg ctcgcggctc ggcgagctgt cgctgccggt gcaggtgatc
     6601 ggcgccatgc gcgacgtgat gatcccgccg caggcgtcgc cggagctggc cgggctgatc
     6661 cccggcgcgc ggctgacgat gctcgagtgc ggccacctcg tgaacgtcga ggcggcccag
     6721 gagttcaaca ggctggtgct cgacttcgcg gccgagcacg ccggcgcgcc cgcctaggcg
     6781 ccggggttgc gcctccgccg ggatcgtgat tagggtggtg cccgtgcccc gcctcgccct
     6841 gatcctggtc gcgctgctcg cagccgcagt gctcgtcccc ggcgccgcct ccgccacgcc
     6901 gatgcaggag agcgcctcgg ccccgtcgcc tggcggcgag gtcgcggccc agctcacgta
     6961 cgacaagcag gacgagttcc agtactcgaa cgtccggatc aagatcacgc gcggcggtgc
     7021 ggtgctgctc gacgccgcgg tgccggctcc ctgcgacgag tgcccgacca gcccgcaggg
     7081 cggcggcgac cccgagatcc cgtcgctggt ggtccgtgac ctcaacggcg atggcgagcc
     7141 cgaggcgctc gtggacctct acacgggcgg agcgcactgc tgctcctaca cgcagatcta
     7201 cggctaccac gcggccacga acgcgtaccg gcgcgtgaag gcgacctggg gcgacttcgg
     7261 ctaccagctg accgacctga acaaggacgg aaagccagag ttccgctcct ccgacttccg
     7321 cttcgccgga gcgttcaccg cgtacgcggc ctcgggcgcg ccgccgatga tcctcaacta
     7381 caccggcggc aagctcgtga acaacacgcg caagttccgc agggagatca agaagaacct
     7441 caagcagtac gtcgcgctct acaaggagat ccgcaaggac ccggacgtgc cggacgtacg
     7501 cggcttcctg gcggcctacg tggccgacaa gtacctgctc ggacagggcg acaccgcctt
     7561 cgacctcgtc tacgcggcgc tccgccgcgg cgagctgaag gccctcgagg gcgacacctc
     7621 gccggccggc aagaagtaca tctccgctct gcgcaagtac ctgcgcaagt ggggctatcg
     7681 ctagcgggcc tccgccagcg atcgcaccac ggcttcgtag aacccctcgc cggccgccgc
     7741 ctcggtgacc tccacgttgg cgccgcccgc cgtcgcgatc gacgggtcct tctcgaccgc
     7801 gttcgccacc aggaagaagc ggcccaccac ctcggccacg cccacgtcct cgcgcgagtc
     7861 gcccaccgcg atgcactcct cgcgcgcgta gccgcgggcg cgcatgtggg tggccaccgc
     7921 gctcgccttc gaggccgtgc gcgggatcag gtggtagcag tgcggcgggc ccggtaggtc
     7981 ctggagggtc tccctcggcg agatcgagcc gttgtccacg agccggagct gaccgtgccc
     8041 gctgctggcc agcagggcgt cggcctcggt ggtgtcgacc aggccgcgga agatgtgtga
     8101 gaactcgcgg ccctcgtgcc acgggtcgtg gtactcgaac cggcccgcgt agcgctcgag
     8161 caggagcgcc ggtgcgccgg actcgtcgat ctgcgcgtag accgtgcggt cggcggtcgg
     8221 ctggagctcg ccggtgaggt agctgtcgtc gccgtcgagg tgcagcccgc agccgacctc
     8281 gtagatgtac gcgctctggc cgagcaggcg cgcgtcctcc atcacctggg ccttgcggcg
     8341 gccggacttg agcacgacct cgacgtcgca ccggtggcac gcctccagcg cccgcgcggg
     8401 cagcagcgtg aaggcgccct cgtggtcgcg gaaaagtgag gcgccgcggc caagcagggt
     8461 cccgtcgaga tcggtgtaca cgcagcggat ggccatcggc tcgggcccta ctatcgcaac
     8521 ggatggccgt cacccggaca cgcgcgccct gggaggggct gatcgagtcc ggccgcgacg
     8581 agcagatcgt ctacgagggg cggctcgcgg cgcgcccggc cgcgcgcgtg ccggtgcccg
     8641 accacctcca cccggaactg ctcgacgccc tggagcgcga cggggtgacc gagctctaca
     8701 cccaccaggc cgacgcgctg tcgagcgctc tcgagggcga cacgatcgtc accaccggca
     8761 cggccagcgg caagtcgctc gcgttcaacc tgccggtgct cgacacgctc gcccgcgacc
     8821 gccgcgcccg cgccttctac ctctacccca cgaaggcgct ggcccaggac caggcgcgca
     8881 agctcaccgg gctgaaggcg ccgttcctca agcacgcgat ctacgacggc gacaccccgc
     8941 agtccgagcg ccgctcgatc cgcgcacgca gcaacctcat cctcaccaac ccggacatgc
     9001 tgaacgtggg agtgctgccc caccaccgcc agtggggcga cgtgctggcc aacctcgcgt
     9061 ggatcgtggt ggacgaggcg cacgtgtacc gcggcgtgtt cggctcccac gtggccaacg
     9121 tgctccgccg gctgcgccgg ctcgcgcgcg cctacggcac cgagccgcgc ttcgtgctcg
     9181 cgagcgcgac gatcgcgaac ccgggcgagc tcgccgagaa gctcaccggc aaggagtgcc
     9241 gcctcgtgga ccgcgacggg gcccctcgcc acgagcgcaa gatcgcgatg tggaacccgc
     9301 ccctgaccga cgaggcgacc ggcaagcggg tctcggtgct gagcgaggcg gcgggcgtgc
     9361 tcgcgaagct ggtcgaggac ggggtgcgga cgatctgctt catgcgctcg cgccgcggcg
     9421 tcgagctggt gcagaacttc acgcggctgc ggctcgagga catgggccgc gccgacctgg
     9481 ccgagcggat cgcgccctac cgcgcgggct acacgcccac gcagcggcgc gacatcgagc
     9541 ggcggctgat ggagggcgac ctgctcgcgg tggtggccac gaacgcgctc gagctgggca
     9601 tcgacgtggg cgacctcgag gcgtcgatct gcatcaccta cccgggcacc gtggcgagcc
     9661 tgcggcagat gtggggccgc gccgggcggc gcgaggccgg cctcgcgatg tacatggcgg
     9721 gcgaggacgc gctcgaccag ttcttctgcc gccaccccga ggacttcctc tcacggccga
     9781 tcgagtcggc gatcctcgac cacgagaacg agtcgatcca cctgccccac ctggtggccg
     9841 ccgcctacga gctgccgctg acaggcgagg accgcgagat cctcggcccg cgctgggagg
     9901 cgtacgccga ccggctcgtg cggatcgggc atctgcgcga gcgcgacggg cgctacctgc
     9961 cacgcggcgc ggagttcccc gcggggcgga tctcgctgcg ctcggcgtcg atcgactcga
    10021 tcgcggtggt ggaggagggg cgcggcgagg tgatcgggac ggtcgagggc gagcgcgcgc
    10081 actcgacggt ccaccccggc gcggtctaca tgcacctcgg ccagagctac gaggtggagg
    10141 agctcgacct gcgcgggcgc agcgcgctcg tgcgctcgtt ctcgggcgac tggtacacgc
    10201 agcccaagaa ggagaccgag accttcatcg aggaggtgcg ccgccagcgg atggtcggga
    10261 ccgtcgagct gaacttcggg atggtctcgg tgagcgagga ggtggtcgcg ttccagaaga
    10321 agcgcgtgag cgaccacgag gtgctcgacc tgctgccggt cgagctgccg atgcagcgct
    10381 tcgtgacgca ggcgctctgg tacgtcatgc ccgaggggct gttcgccgac gacttcccgc
    10441 tcgaggtgct ccaggggtcg ctgcacgcgg ccgagcatgc gcaaatcgcg gtgctgccgc
    10501 tgatcgcgat gtgcgaccgc tgggacatcg gcggactctc gaccgcctac caccaccaga
    10561 ccggccgccc gacgatcttc atctacgacg gccacccggg cggggtcggg atcacgcgcg
    10621 cggggttcga ggcgttcgaa cgcctgtgcg acgacgcgct gctcctgatc tccgagtgtc
    10681 cgtgcaagtc gggctgcccc tcgtgcgtgc agtcgccgaa gtgcgggaac ctcaacgacc
    10741 cgctcgacaa gcacggctcg gtcgagctct tgcagcggat ccgcgcggcc tagcggggca
    10801 agtcctcgac tccaaggcga atcacggtta gcttgttgcg gagtggaggc gacagctaca
    10861 gcgctatttg aatacctccg acgggccgac gcccgccagc acgctagggc cctcgacctt
    10921 agggagcgga tcacagcatg gctcgaatac attccggcca cgtttccgca ctacacgcgc
    10981 cacacggtgg cgcacagcga tgagatcgtc cgccagcttt cagcgctgct cttcgcgtcg
    11041 agtgatcctt cgaagccgac cgtgcagctg tcgtccattg aggcctacgt cctgctcgcc
    11101 gcggcatatc ttcacgacgc cgggatggtt gtatccaaca acgcgaagat ggacgtgctg
    11161 tcgtcctccg aatgggagtc gtggacttcg ggaggcaatc caggcgcgga ccgctggcag
    11221 gctatcctcg cgctgagggc tgaggccgaa gaagatagtt caccgcacct tcacttcgca
    11281 gcggatctcg agacgcggtt cctgctagcg gattatttcc gtcggacgca cgcatcccgg
    11341 gcggcgcagc tgatccaaac gtatcgattg cacctgggtc tcgccgacgc ggatccaaca
    11401 ttggttgaga cgatctcggc ggtgtgtgtc gcacatggtg cgagcatcca cgatctcgag
    11461 gactcggtcc gctaccccga acgtcgcatg acagcgggag agcaggtaaa cgttaggttt
    11521 cttgcactcc ttctccgcct aggtgacctg ttggacatgt caaccgaccg cagttgctca
    11581 ctgctggtca acgcggcagc accgctccca gccgacagtc tggctcattg gacgcagtac
    11641 tccagcatcg tccataggtt gaccgcacct gaccgaatag aggttcgggc gaagtgccac
    11701 gaccatgaag agcatcgagt cttacgcgat tggtgcgatt ggttggctca ggaggtgacg
    11761 aatgccgcgg cgctcatggc acattcggca agacatggcg attggcgggc gcccataact
    11821 aggtcgcggg ggccagaccc gacgatcgaa attgaccgtg cgcaggatgc cacttatgtg
    11881 ctcgaagact ggaagctcga gatcgacgcc gacgccgtgc tgacgcgcct tacggaagat
    11941 atctatggtg aacaaggcgc cttcctaaga gagctgattc agaacgcgct cgacgcgagt
    12001 cgatgccaga tgtacctaga tctccgagcc gaaggaatcg agccgccgcc caatcccgcg
    12061 catgtggaag cagcgtttcg cgacaagtat ggcgtgagag tcgcgatcga cgaaatcctc
    12121 acggagagcc cggtctccgg aaagcgtgag cgccggcacg tcatcacggt cgaggactcc
    12181 ggcattggta tggaccgtga agtcattcaa cgcttttttt tacaaatcgg gcggtcgttt
    12241 tatacgtctc gcgagtttga tcgggtgttc acatttgtgc cgacaagcag gtttggcttg
    12301 gggttcctgt cagtgttcgg cgtaagcgac gatgtaaccg ttgagaccta caagccaagc
    12361 tccgccgctc aggatggtcc tctacgcatg aggctaaccg gctcgaagtc atacttgatt
    12421 accgaagaag gcaaccgtac gacgagtggc actacgatta cagtcgtcct acgcgactta
    12481 cggcctgcga ggtttgtcct tgacaccgtg agaatgtggt gccggatggt cgagctaccg
    12541 attgaggtca gatccttcgg tgaggcagag tgcgtgaccg ccg
//
LOCUS       DASVPL010000147         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_134204, whole genome shotgun sequence.
ACCESSION   DASVPL010000147 DASVPL010000000
VERSION     DASVPL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134204"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VF517_07560"
     CDS             complement(<1..250)
                     /locus_tag="VF517_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052833.1"
                     /translation="MSDDPGVMGNLPRSRPGRRSAKRAGGGSGGAGAKAAGSAKAAGS
                     AKAAGSAKAAGSAKAAGSGASRAKTAGPAKRAGSAGSRA"
     gene            546..1646
                     /locus_tag="VF517_07565"
     CDS             546..1646
                     /locus_tag="VF517_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8052834.1"
                     /translation="MSVAELQELEEVKLLVLKGNQTGVLTYGEVANALTEVDIDDGDI
                     EELHAFLEKSEIELVDDDPALQNANQEERAPDKKGRRAKKAQIDLKPDMTTDSLQLFL
                     KDIGKVRLLTAQEEVDLAKRIERGDLDAKQKMVESNLRLVVSIAKNYRNQGLPFLDLI
                     QEGTLGLVRAAEKFDYRKGFKFSTYATWWIRQAIARALADKARTIRIPVHVVEKLNKI
                     GRAERKLVTELGREPTPEEIAEVTGIEPDEVDSIKRSAQAPVSLEKPVGDEEESEFGQ
                     FIADERAESPYDRAAEILTKEALREALENLSYRERRVLELRYGLGGEHPRTLDEVGRT
                     FNVTRERIRQIENQSLKKLQSLAEAQKLRDVA"
     gene            complement(1650..>2344)
                     /locus_tag="VF517_07570"
     CDS             complement(1650..>2344)
                     /locus_tag="VF517_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052835.1"
                     /translation="NDDVGADVESIAGTPFDDRLIGSDAANGISGLGGRDLIDGRGGT
                     DDLRGSGRIVGGPDHDTISTGDPSPFASGDLIPVGPGVLTLIDTRDVYDDTITCARGS
                     VRLTIDPVDELRLCAPVVVGPRPDDFRVARDGTVRMRLRCASNADVRCAGRVYVRRSG
                     GGPVVGRARFKLPAKGRSAVVAARLTSRYRAELQRARRLRVDLQVVTYRPGPATRKLE
                     KPVPGRTLLAPR"
BASE COUNT          379 a          853 c          802 g          310 t
ORIGIN      
        1 tcgcgcggga cccggcggag ccggcgcgct tcgcggggcc cgccgtcttc gcccgcgacg
       61 cgccggagcc cgcagccttg gcggagcccg cggccttcgc ggagcccgca gccttcgcgg
      121 agcccgcggc cttcgcggag cccgcagcct tcgcgcccgc gccgccgctc ccaccgccgg
      181 cgcgcttcgc gctgcgccgt ccgggccgcg agcgcggcag gttccccatc acgccgggat
      241 cgtcgctcac gccgcagacc ttatcccgac ccgcggcgcg ccccccgcgc gggcgccggc
      301 tacccgcggc ccagcccact cttaacgcgc gtgtgcgaac cgtccgccca ccctgacggt
      361 tttacgtatc aaagccccgg accgggggta tacggaagct cccccggcca gggcgtaccg
      421 gcccaaggcg cccgaccggg accggctgac caaaacggtt gactgcacga aatgaagcga
      481 actagactcg cctcgcctgc tttgattggg cttgcgggag caactcggga gagaaacgga
      541 agggtatgtc ggtagctgaa ctccaggaac tggaggaggt aaagctcctc gtcctcaagg
      601 gcaaccagac gggtgtcctg acgtatggcg aggttgcgaa cgcccttacc gaggtcgaca
      661 tcgacgatgg cgacatcgag gagctgcacg cgttcctcga gaagtccgag atcgagctcg
      721 tcgacgacga ccccgccctc cagaacgcca accaggagga gcgcgcgccc gacaagaagg
      781 gtcgccgtgc caagaaggct cagatcgatc tgaagccgga catgacgacg gattcgctgc
      841 agctcttcct caaggacatc gggaaggtcc gtctgctcac cgcgcaggag gaggtcgacc
      901 tcgccaagcg catcgagcgc ggcgacctcg acgcgaagca gaagatggtc gagtcgaacc
      961 tccggctcgt cgtctcgatc gcgaagaact accgcaacca gggccttccg ttcctcgacc
     1021 tgatccagga gggcacgctc gggctggtcc gcgccgccga gaagttcgac taccgcaagg
     1081 gcttcaagtt ctcgacctac gcgacctggt ggatccgcca ggcgatcgcc cgcgcgctcg
     1141 ccgacaaggc gcgcacgatc cggatcccgg tccacgtggt ggagaagctg aacaagatcg
     1201 gccgcgccga gcgcaagctc gtcaccgagc tgggccgcga gcccacgccg gaggagatcg
     1261 ccgaggtcac cggcatcgag cccgacgagg tcgactcgat caagcgctcg gcccaggcgc
     1321 cggtgtccct ggagaagccg gtcggcgacg aggaggagtc cgagttcggc cagttcatcg
     1381 ccgacgagcg ggccgagtcg ccgtacgacc gcgccgcgga gatcctcacc aaggaggccc
     1441 tgcgcgaggc cctcgagaac ctctcgtacc gcgagcgccg cgtgctcgag ctgcgctacg
     1501 gcctcggcgg cgagcacccg cggacgctcg acgaggtggg ccgcacgttc aacgtcacgc
     1561 gtgagcgcat ccgccagatc gagaatcagt cgctgaagaa gctccagtcc ctcgcggagg
     1621 ctcagaagct ccgcgacgtc gcgtagcgcc tagcgcggcg cgagcagcgt ccggccgggc
     1681 acgggcttct cgagcttgcg cgtcgccggc cccggccggt aggtgaccac ctgtaggtcc
     1741 acccgcagcc gccgggccct ctgcagctcg gcgcggtagc ggctggtcag ccgcgccgcc
     1801 accacagcgc tcctgccctt cgccgggagc ttgaaccgcg cgcgccccac caccggcccg
     1861 ccgccgctgc ggcggacgta gacgcgcccg gcgcaacgga cgtcggcgtt gctcgcgcag
     1921 cgcaggcgca tccgcaccgt cccgtcacgc gcgacgcgga agtcgtcggg ccgcgggccg
     1981 accacgaccg gcgcgcacag tctgagctcg tcgaccgggt cgatcgtgag ccggaccgaa
     2041 ccgcgtgcgc aggtgatcgt gtcgtcgtag acgtcgcgtg tgtcgatcag cgtgaggacg
     2101 ccggggccca ccgggatcag gtcgccgctc gcgaacgggc tcggatcgcc ggtgctgatc
     2161 gtgtcgtggt ccggtccgcc gacgatccgg ccactgcccc ggagatcgtc ggtgccgcca
     2221 cgcccgtcga tcaggtcgcg accgccgaga ccgctgatcc cgttggcggc gtcggagccg
     2281 atcaggcggt cgtcgaacgg agtgccggcg atcgattcga cgtcggcgcc gacgtcgtcg
     2341 ttat
//
LOCUS       DASVPL010000148        33823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_57835, whole genome shotgun sequence.
ACCESSION   DASVPL010000148 DASVPL010000000
VERSION     DASVPL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 33823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33823
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57835"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VF517_07575"
     CDS             <1..1405
                     /locus_tag="VF517_07575"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEX8052836.1"
                     /translation="APPALDEASVTTDPALFPGFDVAVEDYVLRCGPDPVKVTADAPD
                     GVTVAIDGGAPQSGPQSVEVDLEGGQAFRFTIANRAASRTFHVRCLPPNFPGWTVERN
                     GDPAAQWYFASPSFEIQPRSDNPYAIAYDDHGVPVWWRDIAGVPVDVKLLPNGHVAWF
                     RTKVSRYATYPSDAYEERELDGTLVRTLATVGSPTDLHEIQLMPNGNYLMVTYHARDG
                     VDLEPYGGPSDATVLDAEVQELTPAGTLVWSWNAKDHVSLDETGRWWPTVLGTTLTLE
                     DGRVAYDHQHINAVEPDGDGIVISLRHTDAVYRIDRATGAIDWKLGGTPTPASLSFVG
                     DAFAGSAFGGQHDPRVGSDGTLTLFDNGTDRGRPPRAARYAIDPVARTATLVEQVSDP
                     LISGSFCCGSARRLSGGNWVVYWGGTQTFGELSADRRVLLRVTWDNQLSYRVAPVEPG
                     VLSAADLRAAMDAMNPR"
     gene            1538..2098
                     /locus_tag="VF517_07580"
     CDS             1538..2098
                     /locus_tag="VF517_07580"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8052837.1"
                     /translation="MGSLRALAGDLGIPERTLRRAASEGLVRGSRTSPRRFETTVRER
                     WYLHNYWPLLRELRDALRTEPNVRLGVLFGSTATGRAHAASDIDIVVSLADSSMGRIA
                     DLSSRLAARLGRDVQLVRLEDARQAPQLMRALTTQGRVLVDRDGEWPAIVEEAPRWLV
                     RAHRAEKRDALAAYTAAFTEQASRRS"
     gene            2095..2433
                     /locus_tag="VF517_07585"
     CDS             2095..2433
                     /locus_tag="VF517_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052838.1"
                     /translation="MTKAPPAPLEARDAERAQVLKQKVLHRLADVARHHAALLAAMAE
                     FGEDFDLEPFARAHRTDDPTELNRIKAVERGVDQLYNYVAAVVARPGARDGAEPGGRA
                     ERPPRPGGDG"
     gene            2656..5205
                     /gene="clpB"
                     /locus_tag="VF517_07590"
     CDS             2656..5205
                     /gene="clpB"
                     /locus_tag="VF517_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX8052839.1"
                     /translation="MHIDRFTTKSQEALAAAQRLAAARKNPQTSPHHVLAALLEQEGG
                     IVVPVLQHAGVDVTGVRRRANEALDAAPKVTGEDVTAPPVDGITAKVLQNAEEEARGL
                     GDEYVSTEHLLLALASDPKVDVGATRDQLLEAVDEVRGPHRVTDQNPEDKYQALEKFG
                     RDLTRAAEDGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAEGLAQRI
                     VSGDVPSSLKDRRVISLDIGALVAGAKYRGEFEDRLKAVLKEIAEAEGQVILFMDELH
                     TIVGAGAAEGAVDAANLLKPMLARGELRAVGATTLDEYRKHIEKDAALERRFQPVHVG
                     EPSSEDAIAILRGLKERYEVHHGVRIQDSAIVAAAVLSERYIADRFLPDKAIDLIDEA
                     ASRLRIEIDSMPTEIDEVERRVQQLEIELQALKKEKDEASVARREAIDRELAELRERS
                     AGMKAKWQSEKEAIEAIRETKERLEQAHREAERAERDSDLQRAAELRYGEIPELERTV
                     REQEARLSELQSTSTMLKEEVDDEDVAEVVAKWTGIPVSKLMEGEVEKLIHMEERLHD
                     RVVGQDDAVDAVSNALRRSRAGLQDPNRPIGTFLFIGPTGVGKTELARALAEFMFDSE
                     QAMVRIDMSEYMEKHSVSRLVGAPPGYIGYEEGGQLTEAVRRRPYSVILLDEIEKAHT
                     DVFNILLQVMDDGRLTDGQGRTVSFKNAVLIMTSNIPGGREGVEGHFKPEFVNRLDDI
                     VEFDQLSRDEIGRIVAIQVDRLAERVRERGVEIELTEDARTLIGNLGYDPTYGARPLK
                     RVIQRQLVDKLALALLEGRFAAGDTVRVDAHDGELAFERAADAAEQPAAAA"
     gene            5235..5867
                     /locus_tag="VF517_07595"
     CDS             5235..5867
                     /locus_tag="VF517_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8052840.1"
                     /translation="MSQVIVDISMSLDGYVAGPNQSLEEPLGQGGEQLHEWAFATASW
                     REPHGREGGSTGADDELLREARGRIGAEIMGRGKFGGGPGPWDESWQGWWGEDPPFGY
                     PVFVLTHHARETLTLSDTTFTFVTDGIESALEQARAAAGERDVLIGGGADVCNQYIAA
                     GLVDELLLHVAPVMLGGGERLLVGTGAAQLECTQVVGSPQTTHLRYRLQR"
     gene            5864..6760
                     /locus_tag="VF517_07600"
     CDS             5864..6760
                     /locus_tag="VF517_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8052841.1"
                     /translation="MSAAAIQLRDVVKRFGPITAVDGLDLDVPQGICLGLLGPNGAGK
                     STTMRLLTGQAIADEGELRVLGHDLPGESKEARTRMGVVPQLDNLDVDVSVYDNLAIF
                     ARLYRVPDVKATVERGLEIARLTNRAKDSVSELSGGMRRRLLLARGLVHDPELLLLDE
                     PTVGLDPQIRTELWTLIDGLRTQGKTILMSTHYIEEAERLADEVAVMTEGRIVARGRP
                     SDLIREHAGRETAEFYGPPARLAEVRAKAEEQGLRVRPAGPAVAIVGSERASDGAFPA
                     ESIRRAATLEDVFVLLTGEDAE"
     gene            6757..7524
                     /locus_tag="VF517_07605"
     CDS             6757..7524
                     /locus_tag="VF517_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8052842.1"
                     /translation="MRRIEPAAVAGVMSREMANYRTIWKAMTFSSVLEPFVYLIAFGV
                     GLGATIVDRVDGLEYVEFVGTGMVATATIFSSALPAMFGTFVKHHFQRTYDAILAAPV
                     DVEEIVTAEMLWIGLRAGFYGCFPLIAAMAFGLDPAPGMLLVPLFCFVTALAFAAFGI
                     AVAGSVSKIDQFNYVTTLFVTPLFLVAGTFFPIDQLPEGVQIASQFNPLHHLVELVRH
                     ACFGFEATDPLRLLGLIALAVVLWRLAIRQLTQKLID"
     gene            complement(7521..9098)
                     /locus_tag="VF517_07610"
     CDS             complement(7521..9098)
                     /locus_tag="VF517_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8052843.1"
                     /translation="MSRSLPEARSTIRAFLAPETVPPELLGEGIEPLADDAAEAVRSE
                     LGHDEEARLAATAVELLRDVFPSEDQAFEDVENWPLCAALLPHVVSATTHARALGVAH
                     VDSAYVLVAAAAYLMAIDGSKDGARALSERALEIARRAPEPAELAVAEATGQLAYVLH
                     KQGDPHSARPLEEEALDIRVRHQGEDHPETLMAMNNLAVTLEASGDPRGGHVLREAAV
                     KAARRVLGEDDPRTLTMTNNLARTYFAAGDPRRACELDERVLEARRRVLGDEHPKTLS
                     SMSNLAVTLNVLGDARAALRLQEEVLAARRRVLGEDHTMTIQAMSNLGATLHQQGQID
                     RARELHERVAELMDGTPTPAGFTQLNNLAEIRKSHGDLDAARKMHDELLAHARETLGE
                     EHPQTLTAMGNLAATLWLQGEHTEAHAIAQAVVAIRHRTLGAEHPDTLHAMGNLGAML
                     AQGGMLSGARVIYEQLVAGRRTVLGERHPATIAAMEDLARVLRRCGKRREARVVGRRA
                     ASLGGIDPTKLRHRVRG"
     gene            9223..9504
                     /locus_tag="VF517_07615"
     CDS             9223..9504
                     /locus_tag="VF517_07615"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX8052844.1"
                     /translation="MPTYVMMTTLTPEGVQTLKNNPSRILEVNREVEQLGAQVKAQWA
                     VLGQYDFVNVIEAPDEKTMARVSMELGSRGTARYETLVGIPVEDFIATL"
     gene            9501..10784
                     /gene="purD"
                     /locus_tag="VF517_07620"
     CDS             9501..10784
                     /gene="purD"
                     /locus_tag="VF517_07620"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX8052845.1"
                     /translation="MTERVLVIGSGGREHALVRALLRSPQHPEVLAAPGNAGIARDRT
                     PCVDVAVDDVDGIVRVANDEGIDLVVVGPEAPLVAGLVDALGDAGIAAFGPSAAAARI
                     EGSKAYAKELMKDVGVPTASHMVLHSREQAEAQIPCASYPAVLKADVLAAGKGVIICA
                     NEAEAREAIDQFFGQRRFGDTEVVLEEFLDGEELSLLALCDGVRAVPMAPAQDYKRIH
                     DGDLGPNTGGMGSYSPVPGFDPEHARALALAAHQPIVDELRRRGTPYHGVLYAGLMMT
                     AAGPKVLEYNCRFGDPETQAILPRLRSDLVDLLERSTREGGLEGVEIEWSAQWAVTVV
                     LASRGYPESSSEGDAIEGLRGLDTLETEVLHAGTAATDDGFVTAGGRVLNVTGLGDDP
                     DSARTAAYAAADRIQFDGKQLRRDIAARAVERVGA"
     gene            10781..11272
                     /gene="purE"
                     /locus_tag="VF517_07625"
     CDS             10781..11272
                     /gene="purE"
                     /locus_tag="VF517_07625"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX8052846.1"
                     /translation="MSEAPDAGVAPAAMTETEVEAQFDELDVDAPLVGIVMGSKSDMP
                     AMEKASDWLEERGIKSETRVMSAHRDPDTVADYAKNAQMRGLRVIIAGAGLAAALPGV
                     VAAHTQLPVIGVPLTSSTSVAGGLDALLAIAQMPPGVPVACVGVDNARNAAVLAARIL
                     GRT"
     gene            complement(11304..11735)
                     /locus_tag="VF517_07630"
     CDS             complement(11304..11735)
                     /locus_tag="VF517_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8052847.1"
                     /translation="MLRAIVASQIRKGYERVSTARFDELLAGFAGDAVFQMMGEHELG
                     GERRGKAAIRAWFDETHRLFPDLRIEPIAVVASGPPWNMQAATRFRVSASLAGGGRYE
                     NEGMQFLRLRWAKVVEDRLFEDTQVLREAIEHQRAAATSAA"
     gene            11822..13123
                     /gene="purB"
                     /locus_tag="VF517_07635"
     CDS             11822..13123
                     /gene="purB"
                     /locus_tag="VF517_07635"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX8052848.1"
                     /translation="MIARYTRPEIGAVWTDDARMEAWRRVEVACCEEMEGPTADDLAG
                     IRAATFTVEAVNEREKVTDHDMAAFVDVLSESAGPGGRWIHFGLTSSDVLDTALALQV
                     RAAGEIVLRGARELRSALVARAREHRDTLCVGRTHGIHAEPTTFGVKLAGFAFEADRN
                     LNRLERAFEQAAVGAISGAVGTYSATSPDFERRVLERCGLRAEEVSTQVVARDRHAEL
                     VSAIAVAGAGLERFGTEIRHLQRTEVREVEEPFRAGQKGSSAMPHKRNPIVSERLTGI
                     ARLLRGYAQASVENVALWHERDISHSSVERVTLPDATILLDYAQGLALRLVTGMTVRE
                     DRMRANLDATHGALYSQRALLALVESGRTRDEAYRIVQEHAQRAWDTATPFRDLLAEA
                     APELDLDAIFDPSAFVRHAGEIVGRLDALEREPAAAQTAPA"
     gene            complement(13183..13569)
                     /locus_tag="VF517_07640"
     CDS             complement(13183..13569)
                     /locus_tag="VF517_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX8052849.1"
                     /translation="MARKILSEYRTWAVVGWSPRPARDSHRVASFLESRDYRVIRVNP
                     EATGEQGCVPDLASIEEPVDVVDIFRRSELAGVHVDEAIAIGAKAVWMQLGVLDPAAA
                     ARAEAAGLDVVMDRCPAIDHPRMIGV"
     gene            complement(13610..14215)
                     /locus_tag="VF517_07645"
     CDS             complement(13610..14215)
                     /locus_tag="VF517_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052850.1"
                     /translation="MREKYPRVLAANRFFEEETVIHNEVGSNNLNDALSHLGTLFEQA
                     PSMSQVEQAHEVHDFEGHLRRSMMESYELIFRQRMGDVAEKWDQHALIARPLQEQEKL
                     HGVTALRELDTLRRRCKNLVDQGRGCKRGHDWDEWERGTAALIDACQTATDLFYRLDY
                     AIAAAQNHLAEQRRFRVNMAVSASIGVAGIVIGAAVALLAG"
     gene            15814..16701
                     /locus_tag="VF517_07650"
     CDS             15814..16701
                     /locus_tag="VF517_07650"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX8052851.1"
                     /translation="MSTAATLRGHKLLASGKVREIYDEGDRLLMVASDRISTYDVIHP
                     TPIPGKGQVLTGVSAFWFDRTGHIVPNHVVSYTDGVPEQVRGRAMLVERLEMFPVECV
                     VRGYITGSGWKDYQRTGAVCGVELPGGLSESEQLPEPIFTPATKAEVGDHDENVTFDR
                     AAEIIGDRPLLEELRRISIELYTFASEHARERGIILADTKFEFGRDKQGRIVVGDEVL
                     TPDSSRFWPADTYEPGRSQPSFDKQYVRDWASGSGWDKSPPAPALPDDIVAGTSQRYR
                     EAYEKITGESFEAWLQRVS"
     gene            16680..16922
                     /gene="purS"
                     /locus_tag="VF517_07655"
     CDS             16680..16922
                     /gene="purS"
                     /locus_tag="VF517_07655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX8052852.1"
                     /translation="MATARVLIRPKEGILDPQGQTVERALPQLGFQGASHVRIGRLVE
                     LEIDDPSQVPAICEKLLANTLIEDYEVEILSADGRA"
     gene            16919..17608
                     /gene="purQ"
                     /locus_tag="VF517_07660"
     CDS             16919..17608
                     /gene="purQ"
                     /locus_tag="VF517_07660"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX8052853.1"
                     /translation="MRFGVIRFPGSCDEVDALMACRRFAEAELLWHHDRDLKGVDAVV
                     VPGGFSYGDYLRPGALARFSPVMESVANFAKEGGPVLGICNGFQVLCEAGLLPGALLD
                     NAGLKFSCRQVDVIVETDATPFTRACARGERLSIPTKHMSGRYYAPEEQLAQMEAEGQ
                     VVFRYAAGQNPNGSVRDIAGVRNQIGNVVGLMPHPEHAVDPVLGSADGGKLFASAAGA
                     TLPPGREPVPA"
     gene            17605..19830
                     /gene="purL"
                     /locus_tag="VF517_07665"
     CDS             17605..19830
                     /gene="purL"
                     /locus_tag="VF517_07665"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX8052854.1"
                     /translation="MTPPPATAEPRHRQLGLTDAEYELIVEKLGREPNEVELAVFSLM
                     WSEHCAYKHSKKLLGTLPTEGPAVVMGPGENAGAVDVGDGMAVAFKVESHNHPSAVEP
                     FQGAATGVGGILRDVFAIGARPIAILDSLRFGELDSARSRFLLDRAVAGIGHYGNSIG
                     VGTVGGEIYFEGPYEHNCLVNAMCLGIAPQERLTRSAAAGVGNHLVLLGALTGRDGIG
                     GASVLASAELDEEDESKRPSVQIGDPFEEKKLLECCLELLDEGLLESLQDLGAAGLSS
                     SSSEMASKGEVGIDIDVARVPLREADMEPFEVMVSESQERMLCVVAPERLDAVLELCA
                     KWEVRATAIGAVTENRQLRVLDGGEVVGDMPVEALVDDCPLYDLEPQAPTEPVYPAPP
                     ARVARETASDDLLLALVGSPTVASKRWVFEQYDSLVGSRTARRPQAADAAVLLLKQEG
                     EGGALAVSIDGNGRRVACEPYAGTVEAVLECAANLACVGAEPLGLTNCLNFGNPEKPH
                     IAWQLSESVRGLADACRALEVPVVGGNVSLYNEGAEGPIYPTPVVGMVGTLPDPRRAA
                     GIGFAEAGHAIALVGAFAPALAGSEAAKLLGDPLAPLRAVDTTAHARTLDFVREAVRG
                     GRLASAHDVAEGGLAVALAECCIAGGVGARANWASALQAEPGEARLAMFGEGPGGFVV
                     SAPTEALAELAAEAGSVPVVTIGEVGGDLLELTASAARLSLPVEALRSAYEQAIPDLL
                     T"
     gene            19851..21263
                     /gene="purF"
                     /locus_tag="VF517_07670"
     CDS             19851..21263
                     /gene="purF"
                     /locus_tag="VF517_07670"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX8052855.1"
                     /translation="MRDGPRDECGVFGVYAPESDVARLSYFALYALQHRGQESAGIAT
                     AEGGEIIAVRELGLVSQVFDEKTLQALPGGLAIGHVRYSTTGSSAWANAQPIHRSDHH
                     NVALAHNGNLINAVELHAELRERGVGFSSTSDSEIIAALISTHPADSVGEAIEDILPR
                     LEGAFSTVVMTPDGVYAFRDGAGLRPLALGRIGDRYCVASESCAFDIIGAEFLREVLP
                     GELVSLGERGIDTRQVIESDRRSFCVFEHIYFARPDSQLEGRRVQVSRSKMGEILWRD
                     APVDADLVLAVPDSGNPAATGYALASGIPKDDGLIKNRYVARTFIQPGQELRKHGLRM
                     KFNPLPEVVGGKRVVVVDDSIVRGNTTRQIVGMLKDAGATEVHLRISAPPTRHPCHYG
                     IDMSTRQEMIAHERTVEEIARELGADSLAYLTIEGVYEAIESTRETHCDACFTGDYPL
                     ERSEGARGKYALEELAVVPG"
     gene            21291..22631
                     /locus_tag="VF517_07675"
     CDS             21291..22631
                     /locus_tag="VF517_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8052856.1"
                     /translation="MALPPGPRFAPLQAVRFVRNPIDYLTDLQRRHGDAFTVPFPFLG
                     RLVYFAHPDAIRAIFTGDPKLFHSGEAVEPILGPVVGAHSVFVLDEEAHMRERKLLLP
                     PFHGERVRRYAQTFAEITEREVERWPLGAPFALRERMQSLTLEAILRTVFGIHDPAVI
                     ARFQERVPRMGRVGNLVVMLPGLRWDGDRFGPWARFVRAREAVYELIYAEIDRAAADP
                     ALDERDDVLALLLRARREDGSRMGRDELRDELMSVIAAGHETTATGLAWFFERVLRHP
                     EVEERLRAEIAAGDGDEYLDATVRETLRVRPVIVDVVRRLKRRTTIGGWDIPAGTDVV
                     AAAAVVQLREDLYPEPRAFRPERFLDGGGADGAYAWIPFGGGVRRCIGASFAQLELKV
                     IARTILEHAQLSAADARPEPPRAAHVTVVPARGARVVLEQRIAPRAEAVALASA"
     gene            22631..23974
                     /locus_tag="VF517_07680"
     CDS             22631..23974
                     /locus_tag="VF517_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8052857.1"
                     /translation="MARPEPPPGPRFAPLQALHLLREPVTYFERLQRRFGDAFTVPIP
                     LFGKVAYFADPEVVRELFRADPAVFHAGEANADALGPVVGANSVITLDDDAHLRERKL
                     LLPPFHGDRINRYAELFAEIAAREVERWPVGEEFALRPRMQALSLEALLRTVYGVDDD
                     ERVREYHRAVAHMIHVSNIAVWVPALRRDAGRLSPWGRFLRARAAFDRLVHEDIDRGR
                     ADPAALAERDDVLSMLLQAQREDGSLLTREELRDELTTVIVAGHETTATGLSWFFERV
                     LRHPEVEERLRSGDDAYLEATVQEVLRTRPPFFDVVRRLTRDAEVGGYRIPAGMMAGL
                     SIVLVHQRPDVYPEPRAFRPERFLETAPGGFTWIPFGGGVHRCIGASFSLLETRTIAR
                     AILERVRLRPADPAPETPRTNNVMMVPEHGTRVVLEERCVPATSVTTPQRSATAA"
     gene            24017..25837
                     /locus_tag="VF517_07685"
     CDS             24017..25837
                     /locus_tag="VF517_07685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX8052858.1"
                     /translation="MDLDGGLNEWFGFRDFRPGQREACVAAAEGRDVLVVMPTGSGKS
                     LCYQLPALLRDDLTIVVSPLVALMQDQVDALRARGLGDRVAVINAQQDASANAEVLDG
                     AARGELRLLYVAPERFSSPGFFERLSRARIGLFVVDEAHCVSQWGHDFRPDYFRLGDA
                     ARKLGAAGIVASTATATPQVAADVVRRLALREPIRVTTGFDRPNLAFAVARPAPHQKR
                     ALVVEALKREGALPAIVYAGTRAGSNELAEVLTRELGEEALPYHAGLERDRRAAIQRR
                     FLTDDVRVVVATNAFGMGVDKPNVRTVLHSSVPSSLEAYYQEAGRAGRDGKPARALLL
                     AENRDKALHVHFIRRDELREDQPEKLAAVLERDALSDGALPGMSEPRYDVDARELANA
                     AGCRGDHVAALVGHLARAGVVSPTPAPPDRVAGRLLRSVGGRERALCRSSIADAARAR
                     WRQYREVWAYVESESCRRRTILRHFGDHAEPAVHHDAAGNALPCCDACGAADLLPELP
                     RVDTQEAVESLDDAIFSVARTAKPEVGRTTCAEIIHGAKTKKIERNSYDGLAAYATAS
                     HMRRADILARIDELIGEERLATSGGPYPVLKVGPQAAAVA"
     gene            25834..26457
                     /gene="purN"
                     /locus_tag="VF517_07690"
     CDS             25834..26457
                     /gene="purN"
                     /locus_tag="VF517_07690"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX8052859.1"
                     /translation="MSLRVAVLVSGEGSNLQALIDSVHAEGAVEIVGVASNNPGAHGL
                     RRAQAAGVETAAFPASEYPDRTDRDDALAAWLEERDTGLVVLAGFMELLGGEFVRRFS
                     GRIVNVHPSLLPAFPGVRAIEQALDYGVRVMGVTVHFVDEGVDSGPILLQEAFDPVPY
                     ARDIDAVERRVHEIEHRLLPEAVRLIAAGRVHIDPEDPRRVRVDGND"
     gene            26444..28033
                     /gene="purH"
                     /locus_tag="VF517_07695"
     CDS             26444..28033
                     /gene="purH"
                     /locus_tag="VF517_07695"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX8052860.1"
                     /translation="MGTTEGAAPQVTQPGAVRVRRALLTVSDKRGLVDFARGLAELDV
                     EIVSTGGTARELESAGIETRPIDDYTGFPEILDGRVKTLNPRIYAGLLAVRSKPEHVA
                     TLGEQEIEPIDLVCVNLYPFERTAAARGASVEEIVENIDIGGPTLIRAAAKNHGFAAV
                     VTSPESYDAVLEELRMSDGLLSEQTREALAMDAFGYTARYDAAIARWFAEREDDFPAI
                     YPRVYEKVLDLPYGENPHQRAAYYAQANARTHLMSMVSKLHGKELSFNNLLDLDSARR
                     LAHEFEVPAAVIVKHNNPCGCAVGSTPLEAFEKALACDPVSAFGGVFCFNRPIERDLA
                     ERLNEMFVEVVMTKGYEEGALDVLCQKPNPRILHDAERRREPTADRDMRRVRGGLLVQ
                     DRDEDVELRDEMQVVTERKPTEQEWGEMLFAWKVCKHVRSNAIVIAKDLASVGIGAGQ
                     MSRVDSVRIAIDKAQCPLEGAALASDAFFPFADGPQAAIEAGVRAIIQPGGSKRDDEV
                     IGACNDAGVAMVFAARRHFRH"
     gene            28087..28157
                     /locus_tag="VF517_07700"
     tRNA            28087..28157
                     /locus_tag="VF517_07700"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28119..28121,aa:Lys,seq:ttt)
     gene            28310..32953
                     /locus_tag="VF517_07705"
     CDS             28310..32953
                     /locus_tag="VF517_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX8052861.1"
                     /translation="MTARRCNRSAARNALVAIIALAIALLAPSAASASMVALNGTTLA
                     YGGDAVPNTVTISLSGLSYVIDDSVAITPTAPCQNDPTDDTIAECPALTVSSISATLS
                     PTPNALDGNNALVLDSSIGDTTTATVAGGSAVDNFTGQEAAETFNGGSGNDIANGGDG
                     NDPLNGDAGNDTLNGQGGNDTALSGGADSDGIDGGTGNDALNGGGQGDDLVGGDGDDT
                     LRGNNLLNTDAAGDGSDELHGNGDNDILVGHSGPDDLDGGDGVDRADYGDRGATDGVT
                     VTLDNDTAQNDGGALDGTEGNRDIVRTNVENVFGGAGADTITDAVTASTVDNFFVGDA
                     SGDSLYGGLGADQLEGDGGDDVLNGERPGITVESDGDKFFGGANTDTVDYSSRTQDLR
                     IDLENEDLDDGTVVAGVSQENDSVFDDVENVSTGGGNDTVTAQAADVANTFRGGAGAD
                     TLSGDAGADSLLGEAGNDLGLNGNDGADTLSGGDGADQGLAGDAGNDTIDGGEGADAG
                     NGGADVDTISGGAGNDSLSGGGQSDTLNGGADEDTLNGDAGADALNGGTQNDTLNGGA
                     DGDTLHGDDGDDGLNGEAAGANATPSNGDDIHGDAGTDTVTYNRASSGVTVDLDGDDN
                     DDGLQGGSPEGDSVFDTVENIVGTDENDNLTGSGSANRLEGGPGDDTLVGGAGADDLL
                     GGSDSGGVSGGDVLRGGPGPDDLSGGAGDNDVAQYDERTSGVTITIGGTPGDATVPRD
                     DGNADDGAAGARDRVLEDIEDVVGGSGADDITGSNVGNVLTGGLGNDDLDGGLGTDTL
                     NGGGDNDNLNGGPGGASDLGDTLNGGDEEDTADYSTHTADVTLTLDGAASDGATGELD
                     TIAADVENARGGSGNDTVAGNQGENELDGHTGDDTVNGGGGADLVRGGPDNTASPDAG
                     DTVNGEGGNDVLEGGDDADDLNGGSGDDQLDGGTGPDRIDGGLATDTVTYGDRTAAVT
                     VDIDGTVGDVNAPADDGNADDGATATTRDTVYDSNENLVGGSGGDTLTGDGDVNTIDG
                     GAGTDTITGGAGVDVMLGGDDNDEILAVDATVETEIDCGAGTGDIARIDDSPGIVDTT
                     QNCERIDDANDDDDAFPDATDNCDTTKNDDQANFNGDAEGDVCDDTDSDTVFDATDNC
                     RTDANTNQTNTDGDAQGNACDADDDNDGVTDAQEQQRGTNPLVVDSDGDQSNDNADNC
                     PTTPGSQADNDNDGQGDVCDADDDNDGKADGSDNCPATANAEQANADGDAQGDACDSD
                     DDNDGVDDSKDNCRIVGNANQADANGNGAGDACDDDRDGDTIANGNDNCPTVANTDQA
                     NSDADPEGNACDPDDDNDGALDESDDFPTDPARQTKVPLGTNGDDLINGSSLDDTICG
                     LGGDDIINGLQGNDTLFGDACDARQKIGSAQVATAGDDKLTGAEGNDLLFGGGANDKL
                     SGGLGNDSLFGGAGNDRLSGDDGADILLGDDGKDVLSGGSGNDNLQGGAGNDNMNGGQ
                     GKNTFVGGSGNDIINAVNGRKDTIDCGKGRDKLRADRNDKIRRGCERIIKAKG"
     gene            32969..>33823
                     /locus_tag="VF517_07710"
     CDS             32969..>33823
                     /locus_tag="VF517_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX8052862.1"
                     /translation="MTLVRLAYLRRRTLAVLLAFATLACVFACVGQAQAATRASRSDR
                     DADGMRDAWERQHGLNPRSSRDRKRDPDQDHVRNFDEFRQGCDPQAADSDRDGMDDRF
                     EVRYGFDCARRGDQTLDVDGDGLSNRRENDLGTDPTASDTDGDGLSDSDEVRAGSNPV
                     RGDSDRDGDLDGADNCLLVSNADQADRDGDGQGDACTPDADNDGVADPRDNCRTTANP
                     DQRNADGRADGGDACDPDDDDDGTADGSDNCPTAAGPQTDTDGDGKGNPCDEDDDGDG
                     LSDADEAAR"
BASE COUNT         5167 a        12030 c        12054 g         4572 t
ORIGIN      
        1 cgccccgccg gcgctcgacg aggcgagcgt cacgaccgac cccgcgctct tccccggctt
       61 cgatgtcgcg gtcgaggact atgtgctgcg ctgcggcccg gacccggtga aggtgacggc
      121 tgacgccccc gacggggtca ccgtggccat cgatggcggc gcaccgcaga gtgggccgca
      181 gtccgtcgag gtcgacctgg agggcggcca ggcgttccgc ttcacgatcg cgaaccgcgc
      241 ggcgagccgt acgttccacg tgcgctgcct gccgcccaat ttccccggat ggaccgtcga
      301 gcggaacggg gaccccgcgg cgcagtggta cttcgcgtcg ccttcgttcg agatccagcc
      361 gcgctccgac aacccctacg cgatcgcata cgacgaccac ggcgtgccgg tctggtggag
      421 ggacatcgcg ggcgtgcccg tcgacgtgaa gctgctgccc aacgggcatg tcgcctggtt
      481 ccgcaccaag gtcagccgct acgcgaccta ccccagcgac gcctacgagg agcgtgagct
      541 cgacgggacc ctggtccgga ccctggcgac ggtcggctct cccaccgacc tgcacgagat
      601 ccagctcatg cctaacggca actacctcat ggtcacctac cacgctcgcg acggggtgga
      661 cctggagccg tacggcggcc cgtcagacgc gaccgtgctc gacgccgagg tgcaggagct
      721 gactccggcc ggcacgctcg tctggtcgtg gaacgcgaag gaccacgtgt cgctcgacga
      781 gaccggccgc tggtggccga ccgtcctcgg cacgaccttg acgctcgagg acggccgggt
      841 cgcgtacgac caccagcaca tcaacgcggt cgagcccgac ggggacggca tcgtcatctc
      901 gctccggcac accgacgccg tctacaggat cgaccgcgcc accggcgcca tcgactggaa
      961 gctcggcggc acgccgacac ccgccagcct gagcttcgtc ggagacgcct tcgccgggtc
     1021 ggcgttcggc ggccagcacg acccgcgcgt cggctccgac gggacgctca cgctcttcga
     1081 caacgggacc gaccgcggcc ggcccccgcg cgccgcgcgc tacgcgatcg acccggtcgc
     1141 ccgtaccgcc acgctcgtcg agcaggtcag cgacccgctg atctcggggt cgttctgctg
     1201 tggcagcgca cggcggctca gcggcggcaa ctgggtggtc tactggggcg gcacccagac
     1261 cttcggtgag ctgagtgccg atcgcagggt cctgctgcgg gtgacctggg acaaccagct
     1321 ctcctaccgg gtcgcgccgg tggagcccgg cgtgctctcc gccgcggacc tccgcgccgc
     1381 gatggacgcc atgaatccgc gttagctggg tgccctaccg ggcgcacacc gcgtcgcgcc
     1441 gaccgactga gggagaagca tcggccacac gaagtcttca tatcggccat acgagctgga
     1501 cttatcggcc atacaaagat aatttatcgg ccacacgatg ggttcactac gcgcactggc
     1561 tggggacctc gggatccccg agcggacgct gcgtcgtgcc gcatcggagg gcctcgtgcg
     1621 aggctcgcgc accagcccac ggcggttcga gaccacggtt cgcgaacggt ggtacctgca
     1681 caactactgg cccctcctgc gcgagctgcg cgacgctctc cgcaccgagc ccaatgtgag
     1741 gctgggggtg ctgttcggct cgacggccac aggcagggcg cacgccgcct ccgacatcga
     1801 catcgtcgtc tcgctggcgg attcgtcgat gggccggatc gccgatctct cttcacgctt
     1861 ggcggcgcgg ctgggacgcg acgtccaact ggtccgcctc gaggacgcgc ggcaggcccc
     1921 gcagctcatg cgggcactca cgacccaagg acgtgtgctg gtcgatcgcg atggcgaatg
     1981 gccggccatc gtggaagagg ctccacgctg gctagtgcgt gcgcacaggg cggagaagcg
     2041 cgatgcactg gcggcgtaca cggccgcgtt cacggagcaa gccagcaggc gatcgtgacc
     2101 aaggctccgc ccgcgccact cgaagctcgc gatgcggagc gcgcgcaggt tctgaagcag
     2161 aaggtcctgc accgcctggc cgatgtggcg aggcaccacg cggcgcttct cgccgcgatg
     2221 gccgaattcg gcgaggactt cgacctcgaa cccttcgcca gggcccacag aaccgatgac
     2281 ccaacagagc tcaaccgcat caaggccgtg gagcgtggtg tcgaccagct ctacaactac
     2341 gtggccgctg tcgtcgctcg gcctggagct cgcgatggag cggagccggg aggacgcgct
     2401 gaacgcccgc ctcgacctgg aggcgatggc tgacaacggt gtgctcactc ggcagttggt
     2461 gacgcggctc cagcgcctgc gtgtcctgcg tcgtgacctc gtgcacgagt gcccgacgcc
     2521 aaccgcggag caggtccacg aagcggcgcg actcgtggcc gcggacttcc ctcgattccg
     2581 gaatacgtac ctgaggtggg tgtgttcgca ttgacgaagc cccgctgacc ttctataaca
     2641 cgacttagat tttctatgca catcgatcgc ttcaccacca agtcccagga ggccctcgcg
     2701 gccgctcagc ggctcgccgc cgcgcgcaag aacccgcaga cctcgcccca ccacgtgctc
     2761 gccgcgctgc tcgagcagga gggcgggatc gtcgtgccgg tgctccagca cgcgggcgtg
     2821 gacgtgaccg gcgtccgccg ccgcgccaac gaggcgctcg acgccgcgcc gaaggtcacc
     2881 ggcgaggacg tgaccgcacc gccggtcgac ggtatcaccg ccaaggtcct ccagaacgcc
     2941 gaggaggagg cgcgcggcct cggcgacgag tacgtctcca ccgagcacct gctgctcgcg
     3001 ctcgcctccg acccgaaggt cgacgtcggc gccacgcgcg accagctcct cgaggccgtc
     3061 gacgaggtcc gcggcccgca ccgcgtcacc gaccagaacc ccgaggacaa gtaccaggcg
     3121 ctcgagaagt tcggtcgcga cctcacgagg gccgccgagg acggcaagct cgaccccgtc
     3181 atcggccgcg acgacgagat ccgccgcgtg atccaggtcc tctcccgccg cacgaagaac
     3241 aaccccgtgc tgatcggcga gccgggcgtc ggcaagaccg cgatcgccga gggcctcgcc
     3301 cagcgcatcg tctccggcga cgtgcccagc tcgctcaagg accgccgcgt gatctcgctc
     3361 gacatcggtg cgctggtcgc cggcgcgaag taccgcggcg agttcgagga ccggctgaag
     3421 gccgtgctga aggagatcgc cgaggccgag ggccaggtga tcctgttcat ggacgagctg
     3481 cacacgatcg tgggcgccgg cgcggccgag ggcgccgtgg acgccgccaa cctgctcaag
     3541 ccgatgctcg cccgcggcga gctgcgcgcg gtcggcgcca ccacgctcga cgagtaccgc
     3601 aagcacatcg agaaggacgc cgcgctcgag cgccgcttcc agccggtgca cgtgggcgag
     3661 ccgagctcgg aggacgccat cgcgatcctg cgcggcctca aggagcgcta cgaggtgcac
     3721 cacggcgtgc gcatccagga ctccgcgatc gtcgccgcgg cggtgctgag cgagcgctac
     3781 atcgccgacc gctttctgcc cgacaaggcg atcgacctga tcgacgaggc cgcgtcgcgg
     3841 ctgcgcatcg agatcgactc gatgcccacc gagatcgacg aggtcgagcg ccgcgtgcag
     3901 cagctcgaga tcgagctcca ggcgctgaag aaggagaagg acgaggcgtc ggtggcccgc
     3961 cgcgaggcga tcgaccgcga gctggccgag ctgcgcgagc gctccgccgg gatgaaggcc
     4021 aagtggcagt ccgagaagga ggccatcgag gcgatccgcg agaccaagga gaggctcgag
     4081 caggcccacc gcgaggccga gcgcgccgag cgcgactccg acctccagcg cgccgccgag
     4141 ctgcgctacg gcgagatccc cgagctcgag cgcaccgtgc gcgagcagga ggcccgcctc
     4201 tcggagctcc agtccacgag cacgatgctc aaggaggagg tcgacgacga ggacgtggcc
     4261 gaggtcgtgg ccaagtggac cggcatcccg gtgtcgaagc tgatggaggg cgaggtcgag
     4321 aagctcatcc acatggagga gcgcctgcac gaccgcgtgg tcggacagga cgacgcggtc
     4381 gacgcggtct cgaacgcgct gcgccgctcg cgcgccggcc tccaggaccc gaaccgcccg
     4441 atcggcacgt tcctgttcat cggcccgacc ggcgtcggca agaccgagct ggcccgcgcg
     4501 ctcgccgagt tcatgttcga ctccgagcag gcgatggtcc gcatcgacat gtccgagtac
     4561 atggagaagc actcggtctc gcggctcgtg ggcgcgccgc ccggctacat cggctacgag
     4621 gagggcggcc agctcaccga ggcggtccgc cgccggccgt actccgtgat cctgctcgac
     4681 gagatcgaga aggcccacac cgacgtcttc aacatcctgc tccaggtgat ggacgacggc
     4741 aggctgaccg acggccaggg ccgcacggtc agcttcaaga acgccgtcct gatcatgacc
     4801 tcgaacatcc ccggcgggcg cgagggcgtg gagggccact tcaagcccga gttcgtgaac
     4861 cgcctcgacg acatcgtcga gttcgaccag ctctcgcgcg acgagatcgg gcggatcgtg
     4921 gcgatccagg tggaccgcct cgccgagcgc gtgcgcgagc gcggcgtgga gatcgagctg
     4981 accgaggacg cgcggacgct gatcggcaac ctcggctacg acccgaccta cggcgcgcgg
     5041 ccgctgaagc gcgtgatcca gcgccagctc gtggacaagc tcgcgctggc gctgctcgag
     5101 ggccggttcg ccgcgggcga cacggtcagg gtggacgccc acgacggcga gctggcgttc
     5161 gagcgcgccg ccgacgccgc cgagcagccc gcagccgccg cttaatttcg gcgtctgcga
     5221 ccgtctcagt aggtatgagc caagtcatcg tcgatatcag catgtccctg gacggctacg
     5281 tggccggccc gaatcagtct ttggaggagc cgctcggcca gggcggggag cagttgcacg
     5341 agtgggcgtt cgccacggcg agctggcgtg agccgcacgg ccgcgagggc ggcagcaccg
     5401 gcgccgacga cgagctgctg cgcgaggcgc gcggccggat cggcgcggag atcatggggc
     5461 gcggcaagtt cggcggcggg cccgggccgt gggacgagtc gtggcagggc tggtggggcg
     5521 aggacccgcc gttcggctat cccgtgttcg ttctcaccca ccacgcgcgc gagacgctca
     5581 cactgagcga cacgaccttc acgttcgtca ccgacgggat cgaatccgcc ctagagcagg
     5641 ctcgcgcggc cgcgggcgag cgtgatgtgc tgatcggcgg cggcgccgac gtctgcaacc
     5701 agtacatcgc ggccggcctg gtggacgagt tgctgctgca tgtcgcgccg gtgatgctcg
     5761 gcggcggcga gcggctcctc gtgggcacgg gcgccgctca gctcgagtgc acgcaggtgg
     5821 tcggctcgcc gcagaccacc cacctccgct accgcctcca gcggtgagcg ccgcggcgat
     5881 ccagctccgg gacgtcgtga agcggttcgg gccgatcacg gcggtggacg ggctcgacct
     5941 cgacgtgccg cagggcatct gcctcggcct gctcgggccg aacggcgccg gcaagtccac
     6001 gacgatgcgc ctgctcaccg ggcaggcgat cgccgacgag ggcgagctgc gcgtgctggg
     6061 ccacgatctt ccgggcgagt cgaaggaggc gcggacgcgc atgggcgtcg tgccgcagct
     6121 cgacaatctc gacgtggacg tgtcggtcta cgacaacctc gcgatcttcg cgcggctcta
     6181 ccgcgtgccc gacgtgaagg cgaccgtcga gcgcggcctc gagatcgctc gcctgacgaa
     6241 ccgcgccaag gactcggtct ccgagctgtc cggcggcatg cgccggcgcc tgctgctcgc
     6301 gcgcggcctc gtgcacgatc ccgagctgct gctgctggac gagcccaccg tcggcctcga
     6361 cccgcagatc cgcaccgagc tgtggaccct gatcgacggc ctgcgcaccc agggcaagac
     6421 gatcctgatg tccacgcact acatcgagga ggccgagcgg ctggccgacg aggtggcggt
     6481 gatgacggag ggccggatcg tcgcgcgcgg ccgcccgagc gacctgatcc gcgagcacgc
     6541 gggccgcgag accgcggagt tctacggtcc gcccgcgcgc ctggccgagg tgcgcgcgaa
     6601 ggcggaggag cagggcctgc gggtgcgccc ggccggcccg gccgtcgcga tcgtcgggtc
     6661 cgagcgcgcg agcgacggag ccttcccggc ggagtcgatc cgccgcgcgg ccacgctcga
     6721 ggacgtgttc gtcctgctga ctggggagga cgccgagtga gacgcatcga gccggccgcc
     6781 gtggcgggag tgatgagccg cgagatggcg aactaccgga cgatctggaa ggcgatgacg
     6841 ttctcgagcg tgctcgagcc gttcgtctac ctgatcgcct tcggcgtcgg gctcggggcg
     6901 acgatcgtcg accgcgtgga cggcctcgag tacgtcgagt tcgtcggcac cgggatggtc
     6961 gcgacggcca cgatcttctc gagcgcgctg ccggcgatgt tcggcacgtt cgtcaagcac
     7021 cacttccagc gcacctacga cgcgatcctc gccgcgcccg tcgacgtcga ggagatcgtg
     7081 accgcggaga tgctctggat cggcctgcgc gccggcttct acggctgctt cccgctgatc
     7141 gccgcgatgg cgttcgggct cgatcccgct ccgggcatgc tgctcgtgcc gctgttctgc
     7201 ttcgtcaccg cgctcgcgtt cgccgcgttc gggatcgccg tggcggggag cgtctcgaag
     7261 atcgaccagt tcaactacgt gacgacgctg ttcgtcacgc cgctgttcct ggtcgccggc
     7321 acgttcttcc cgatcgacca gctcccggag ggcgtgcaga tcgcctcgca gttcaacccg
     7381 ctccaccacc tggtcgagct ggtccgtcac gcgtgcttcg gcttcgaggc gaccgacccg
     7441 ctgcggctgc tcggcctcat cgcgctggcc gtggtgctct ggcggctggc gatccgccag
     7501 ctgacgcaga aattgatcga ctagccgcgc acgcggtggc gcagcttggt cgggtcgatc
     7561 ccgccgagcg acgcggcgcg gcggcccacg acgcgcgcct cgcggcgctt gccgcagcgg
     7621 cgcagcacgc gggcgaggtc ctccatcgcg gcgatcgtcg ccgggtggcg ctcgccgagt
     7681 accgtccggc ggccggccac gagctgctcg tagatcacgc gtgcgccgga gagcatcccg
     7741 ccctgcgcga gcatcgcgcc gaggttgccc atcgcgtgga gcgtgtccgg atgctcggct
     7801 ccgagcgtgc ggtggcggat cgcgacgacg gcctgcgcga tcgcgtgcgc ctcggtgtgc
     7861 tcgccctgca gccagagcgt cgcggcgagg ttgcccatcg ccgtcagcgt ctgcgggtgc
     7921 tcctcgccga gggtctcgcg tgcgtgcgcg agcagctcgt cgtgcatctt ccgcgcggcg
     7981 tcgaggtcgc cgtgcgactt gcggatctcg gccaggttgt tgagctgggt gaagccggcc
     8041 ggggtggggg tgccgtccat cagctcggcc acgcgctcgt gcagctcgcg ggcgcggtcg
     8101 atctgcccct gctggtggag cgtggcgccg aggttgctca tcgcctggat cgtcatcgtg
     8161 tggtcctcgc cgagcacgcg cctgcgcgcc gcgagcacct cctcctggag ccgcagcgcg
     8221 gcgcgggcgt cgccgagcac gttcagggtc accgcgaggt tcgacatcga gctgagcgtc
     8281 ttcgggtgct cgtcgcctag cacgcggcgg cgggcctcca gcacgcgctc gtcgagctcg
     8341 cacgcgcggc gcgggtcgcc ggcggcgaag taggtgcggg ccaggttgtt ggtcatcgtg
     8401 agcgtgcggg ggtcgtcctc gccgagcacc cggcgagcgg ccttcaccgc ggcctcgcgg
     8461 agcacgtggc cgccgcgggg gtcgccgctg gcctcgaggg tcacggcgag gttgttcatc
     8521 gccatcaggg tctcggggtg gtcctcgccc tggtggcgca cgcggatgtc gagcgcctcc
     8581 tcctcgagcg gccgcgcgct gtgcgggtcg ccctgcttgt gcagcacgta ggccagctgg
     8641 ccggtcgcct cggcgacggc gagctcggcc ggctcggggg cgcggcgggc gatctcgagc
     8701 gcgcgctcgg agagagcgcg agcgccgtcc ttcgacccgt cgatcgccat caggtaggcg
     8761 gccgcggcga cgaggacgta cgcggagtcc acgtgggcga cgccgagggc gcgggcgtgc
     8821 gtggtcgcgc tcaccacgtg gggcagcaga gccgcgcaca gcggccagtt ctccacgtcc
     8881 tcgaaggctt ggtcctcgct cgggaagacg tcgcgcagca gctccacggc cgtggctgcg
     8941 agccgggcct cctcgtcgtg gccgagctcg gagcggaccg cctcggccgc gtcgtcggcc
     9001 agcggctcga tgccctcacc gagcagctcc ggcgggaccg tctcgggggc gaggaacgcg
     9061 cggatcgtcg accgcgcctc gggcaggctg cggctcatcc cctccagatc gcgcgcacgc
     9121 gtggaatcca tgcctcgcga gtcggcggat cgggcggatt ccttgagggc agaccccgta
     9181 gtaccgtggc gcggcgagca ccaccgaccc aagggagtag cagtgccgac ctacgtgatg
     9241 atgaccaccc tcacccccga gggagtccag acgctgaaga acaacccgtc ccggatcctc
     9301 gaggtcaacc gggaggtcga gcagctcggc gcgcaggtga aggcgcagtg ggccgtgctc
     9361 ggccagtacg acttcgtgaa cgtgatcgag gcgcccgacg agaagacgat ggcgcgcgta
     9421 tcgatggagc tcggctcgcg cgggaccgcc cgctacgaga cgctcgtggg catcccggtc
     9481 gaggacttca tagccaccct ttgactgagc gagtcctcgt aatcggctcg ggcgggcgcg
     9541 agcacgcgct cgtgcgcgcg ctgctgcgca gtccccagca cccggaggtg ctggccgccc
     9601 ccggcaacgc ggggatcgca cgcgacagga cgccgtgcgt ggacgtggcc gtcgacgacg
     9661 tcgacggcat cgtgcgtgtg gcgaacgacg aggggatcga cctggtggtg gtcgggccgg
     9721 aggcgccgct ggtcgccggt ctcgtggacg cgctcggcga cgcggggatc gcggcgttcg
     9781 gcccgtcggc ggccgccgcg cggatcgagg gctcgaaggc gtacgcgaag gagctgatga
     9841 aggacgtggg ggtgccgacc gcctcgcaca tggtcctgca cagccgtgag caggcggagg
     9901 cgcagatccc gtgcgcgtcg tacccggcgg tgctcaaggc cgacgtgctg gccgccggca
     9961 agggcgtgat catctgcgcg aacgaggccg aggcgcgcga ggcgatcgac cagttcttcg
    10021 gccagcgccg gttcggcgac accgaggtcg tgctcgagga gttcctcgac ggagaggagc
    10081 tgtcgctgct ggcgctctgc gacggcgtcc gcgcggtgcc gatggccccg gcccaggact
    10141 acaagcggat ccacgacggc gacctcggcc cgaacaccgg cgggatgggc tcgtactcgc
    10201 cggtccccgg cttcgacccc gagcacgccc gcgcgctggc gcttgccgcc caccagccga
    10261 tcgtcgacga gctgcgccgc cgcggcacgc cctaccacgg ggtgctctac gccgggctga
    10321 tgatgaccgc ggccgggccg aaggtgctcg agtacaactg ccgcttcggc gacccggaga
    10381 cgcaggcgat cctgccgcgg ctgcgcagcg atctcgtcga cctgctcgag cgcagcacgc
    10441 gcgagggcgg gctcgagggg gtcgagatcg agtggtccgc gcagtgggcg gtcacggtgg
    10501 tgctcgcctc gcgcgggtac ccggagtcgt cctcggaggg cgacgcgatc gaggggctcc
    10561 gcgggctcga cacgctcgag accgaggtgc tccacgcggg caccgccgcg accgacgacg
    10621 gcttcgtgac cgcgggcggg cgcgtgctca acgtgacggg gctcggggac gacccggact
    10681 ccgcgcggac ggccgcgtat gcggcggcgg accgaatcca gttcgatggc aagcaactac
    10741 gacgcgacat cgccgcgcga gcggtggaaa gggtgggtgc atgagcgagg cgccggatgc
    10801 cggggttgcc ccggcggcca tgaccgagac cgaggtggag gcgcagttcg acgagctcga
    10861 cgtggatgcc ccgctcgtgg ggatcgtgat gggctcgaag agcgacatgc ccgcgatgga
    10921 gaaggcgagc gactggctcg aggagcgcgg gatcaagtcg gagacgcgcg tgatgagcgc
    10981 gcaccgcgat cccgacacgg tcgccgacta cgcgaagaac gcgcagatgc gcgggctgcg
    11041 ggtgatcatc gcgggcgccg ggctggcggc ggcgctgccg ggggtcgtgg cggcgcacac
    11101 gcagctgccg gtgatcggcg tgcccctgac cagctcgacc tcggtggccg gcggcctcga
    11161 cgcgctgctg gcgatcgcgc agatgccgcc gggcgtcccg gtcgcgtgcg tgggcgtgga
    11221 caacgcgcgc aacgccgcgg tgctggccgc gcggatcctg gggcggacct aggcggggcg
    11281 gcagggcagc gcccctcggc gcgctacgcg gcgctggtgg ccgcggcgcg ctggtgctcg
    11341 atcgcctcgc ggagcacctg ggtgtcctcg aacaggcggt cctcgacgac cttcgcccag
    11401 cgcaggcgca ggaactgcat gccctcgttc tcgtagcggc cgccgccggc cagtgaggcg
    11461 ctcaccctga agcgggtcgc ggcctgcatg ttccagggcg ggccgctcgc cacgaccgcg
    11521 atcggctcga tcctcaggtc ggggaacagg cggtgcgtct cgtcgaacca cgcgcggatg
    11581 gccgccttcc cgcggcgctc cccgccgagc tcgtgctcgc ccatcatctg gaagaccgcg
    11641 tcgccggcga agcccgcgag cagctcgtcg aacctcgcgg tgctgacgcg ctcgtacccc
    11701 ttccggatct gactcgccac gatcgccctc aacatgcgct gcctcccccg ttcgcctccg
    11761 gggatgttct caccccgaga gcgttgctag gtgacgcccg tcacggcggt atcttcgact
    11821 cgtgatcgcg cgctacacgc gtccggagat cggggccgtc tggacggacg atgctcggat
    11881 ggaggcgtgg cggcgggtcg aggtggcgtg ctgcgaggag atggagggcc cgacggccga
    11941 cgacctcgcc ggaattcggg ccgcgacgtt caccgtcgag gccgtgaacg agcgcgagaa
    12001 ggtgaccgac cacgacatgg cggcgttcgt ggacgtcctg tcggagtcgg ccgggcccgg
    12061 cgggcgctgg atccacttcg ggctcaccag ctcggacgtg ctcgacaccg ccctggcgtt
    12121 gcaggtccgc gccgcgggcg agatcgtcct gcgcggtgcg cgcgagctgc gctcggcgct
    12181 cgtcgctcgg gcgcgcgagc atcgcgacac gctctgcgtc gggcgcaccc acggaataca
    12241 cgcggagccg accacgttcg gcgtgaagct cgccgggttt gcgttcgagg cggatcgcaa
    12301 cctgaaccgg ctcgagcgcg cgttcgagca ggcggcggtg ggcgcgatat cgggcgcggt
    12361 gggcacatat tcggccacgt cgcccgactt cgagcgccgg gtgctcgagc gttgcggcct
    12421 gcgggcggag gaggtgtcca cccaggtggt cgcgcgcgac aggcacgcgg agctggtctc
    12481 ggcgatcgcg gtcgccgggg cggggctcga gcgcttcggc accgagatcc gccacctgca
    12541 gcgcaccgag gtgcgggagg tcgaggagcc gttccgggcg gggcagaagg ggtcgagcgc
    12601 gatgccgcac aagcgcaacc cgatcgtgtc cgagcgtctg accgggatcg cgcggctgct
    12661 gcgcggctac gcgcaggcgt ccgtcgagaa cgtggccctg tggcacgagc gcgatatctc
    12721 gcactcgtcg gtcgagcggg tcacgctgcc cgacgcgacg atcctgctcg actacgcgca
    12781 ggggctcgcc ctgcggctcg tcaccgggat gacggtgcgc gaggaccgca tgcgggcgaa
    12841 tctggacgcc acccacggcg ccctctactc gcagcgcgcg ctgctggcgc tcgtcgagtc
    12901 gggccgcacg cgcgacgagg cctaccggat cgtccaggag cacgcgcagc gcgcctggga
    12961 caccgccact ccgttccgcg acctcctcgc ggaggccgcg cccgagctcg acctcgacgc
    13021 gatcttcgac ccgagcgcct tcgtcaggca cgcgggcgag atcgtggggc ggctcgacgc
    13081 gctggagcgc gagccggcgg ccgcgcagac cgctccggcc tagaccctcg gctgcgaagg
    13141 tcccttctga acacttgacc accccatagg ggcgttaagt gttcagaccc cgatcatccg
    13201 cgggtggtcg atcgccgggc agcggtccat caccacgtcg agcccggccg cctcggcgcg
    13261 ggccgccgcc gcggggtcga gcacgccgag ctgcatccag accgccttgg ccccgatcgc
    13321 gatcgcctcg tccacgtgga caccggccag ctcgctgcgg cggaagatgt ccacgacgtc
    13381 cactggctcc tcgatcgacg ccaggtccgg gacgcaaccc tgctcgccgg ttgcctccgg
    13441 gttcacccga atcacccggt aatcgcggct ttccaagaag ctcgccaccc ggtgcgagtc
    13501 acgcgccggc cgcggcgacc agcccaccac ggcccaggtc cggtactccg acaggatctt
    13561 tcgcgccatc tcttcattcg ccccggaacc catcagaagc cgttcgtccc tagccagcca
    13621 gcaatgccac ggcagcgccg atgacgatcc ccgcaactcc gatgctcgcc gaaaccgcca
    13681 tgttgaccct gaaccgcctc tgttcggcta gatgattttg agcagcggca atggcgtagt
    13741 ccaagcggta aaacaggtcc gtggcggtct gacacgcatc gatcagtgcg gccgtccccc
    13801 gctcccactc gtcccagtcg tgtccgcgct tgcacccgcg cccctgatca acgaggttct
    13861 tgcagcgccg ccgaagggtg tccaactcgc gcagcgccgt caccccatgc aatttttcct
    13921 gttcctggag aggccgggcg atcaaggcgt gctggtccca cttttccgca acgtcgccca
    13981 ttcgctgtcg aaaaatgagt tcgtacgact ccatcatgct ccgtcgcaaa tgcccctcga
    14041 agtcgtgcac ctcatgagcc tgctcgacct ggctcatcga gggggcctgt tcaaaaaggg
    14101 tccctaagtg cgataaggcg tcgttgaggt tgttcgatcc gacctcgttg tgtatgaccg
    14161 tttcttcttc gaagaaccgg ttcgccgcaa gcacgcgggg gtacttctcc ctcatgaccc
    14221 atatgacccg ggcaaggctg tccacgtaga cgccctcgac tcgggcgtcc gacgcggttg
    14281 tcttttcgcc ccctgggcgc cgtacttcgt tcgacatctt ggttcggttt cgctcgggct
    14341 acggctgcgc ggtcgcgcgg catcttaaat gcttaggccg tcactcaagc ggccagcgca
    14401 gcacagcttc taccgctcag tatcggcgat gtgttcttcg tcaggcgttt gctctaggaa
    14461 cacatcgcca cggtctcgaa gccgatctct ttcttcatct tcgaggtgcc gtaggtgtag
    14521 gtcgttccgg ccgccaagat cggacacgag tggcgcgtag cgcacacgct gacgacgagg
    14581 aatacgttga acgtccgcaa tcgttatttc gtcagcagac tttccggttt cttgctcaat
    14641 ccaatcttcc attgggacag cgacgtgagc ggaaggatgg gtttcgaccg ggccagcggc
    14701 gctgcgcggg ttccgccaaa acttaagcct cacgctcatc acctgctccg actcggaggc
    14761 tccgcacagc ggcacgtcgg actagaggtt gccgtcgcgg cccatcgaga ttggcgactc
    14821 ggggcggatg gccagaacgt gttgatgatg gtgctacgag cattcgctgt ttccacgacc
    14881 cgacgggtga gcgccgggcg ggcaatcttg actcgggatg aagcgccggc cgacccagga
    14941 gatgatcagc cgcgcggaac ctgcgagccc atccttgcag atcggatccg tccgcgtcca
    15001 ctgccacact tcgatgtttt gctccacgct cgaccagcgc ttgacgtcgc gaggtcccgc
    15061 tgatgcggct caagatcggc gaggctggct gcttctcctg ccgtcgttgc cgctgtctct
    15121 ctcttaccat gagctgtggc atacatcatg gctttggcgc cagacggaag agcattactc
    15181 gcagaacgcc cggtttacct gcggatcgcc cgcgcttgcg cgcgcgatgc ctgcacggtg
    15241 catgcgctca cgcgcgaggt agatgctgct gactcgccgc tgagatacgc cgctgaacgt
    15301 ctagcggatg ccggcgttct gacccggacc tccgggagca gggcgaattc tcgcgcctac
    15361 cgccttagcg aggcaggcgc gcttctcatc tcggaaattg agcgcactgc ggcgcccggc
    15421 cagttggatg cctcgatgcg tgccattctg atccccgccc aagaccttct caggattgct
    15481 cggtgtgtgt ccgcccacaa ggtggcgcag aagatcgggt gggcgatccg tttccgggac
    15541 tcaaccgctg ggcttctcgt cgttcccgcc cagggatgct ccgatcggga cgtagacgag
    15601 ctcagcgcgg cttttgctgg tgccggctgt tcgccgacca cagtcacggt cggcgaggta
    15661 ctccgccacg ctgaatttat ggactactgc cgtgtgctcg cagcggcagc caacccttcg
    15721 ctcccggaac cgggtgcctg acacgaggac atgctgagcc gaagccgcgg agtcagcgcg
    15781 ccgccgacca ctccgactgt tagcctcggg gccgtgagta cggccgccac ccttcgaggc
    15841 cacaagctgc ttgcgtcggg caaggtccga gagatatacg acgagggcga ccggctgctg
    15901 atggtggcgt cggatcgcat ctccacatac gacgtgatcc atcccacgcc gatccccggc
    15961 aagggccagg tgctgaccgg agtgtccgcg ttctggttcg accggaccgg ccacatagtg
    16021 ccgaaccacg tcgtctccta cacggacgga gtgcccgagc aggtgcgcgg gcgcgcgatg
    16081 ctggtcgagc gcctcgagat gttcccggtc gagtgcgtcg tgcgcgggta catcaccggc
    16141 tcgggctgga aggactacca gcgcacggga gccgtgtgcg gggtcgagct gccgggcggg
    16201 ctcagcgagt ccgagcagct tccggagccc atcttcaccc cggccacgaa ggccgaggtg
    16261 ggcgaccacg acgagaacgt cacgttcgac cgtgcggccg agatcatcgg cgaccggccg
    16321 ctgctcgagg agctgcgccg gatctcgatc gagctctaca cgttcgcctc cgagcacgcg
    16381 cgcgagcgcg ggatcatcct cgccgacacc aagttcgagt tcggccgcga caagcagggc
    16441 cgtatcgtgg tgggcgacga ggtgctcacg ccggactcgt cgcgcttctg gccggccgac
    16501 acgtacgagc cgggcaggtc gcagccctcg ttcgacaagc agtacgtgcg cgactgggcg
    16561 agcggctcgg gctgggacaa gtcgccgccc gcgccggccc tgcccgacga catcgtcgcg
    16621 ggcacgtcgc agcgctaccg cgaggcctac gagaagatca ccggcgagtc gttcgaggcg
    16681 tggctacagc gcgtgtcctg atccggccca aggaggggat cctcgatcct cagggccaga
    16741 cggtcgagcg cgcgcttccg cagctcggct tccagggcgc ctcgcacgtg cggatcggcc
    16801 ggctggtcga actcgagatc gacgacccct cccaggtacc ggcgatctgc gagaagctcc
    16861 tcgcgaacac gctgatcgag gactacgagg tcgaaatcct gagcgcggac ggccgcgcgt
    16921 gaggttcggc gtcatccgct tcccgggctc ctgtgacgag gtcgacgcgc tgatggcgtg
    16981 caggcgcttc gccgaggccg agctgctctg gcatcacgac cgcgacctca agggcgtcga
    17041 cgcggtcgtc gtccccggcg ggttctccta cggcgactac ctccgccccg gcgccctggc
    17101 ccgcttctcg ccggtgatgg aatcggtggc gaacttcgcc aaggagggcg gcccggtgct
    17161 cggcatctgc aacggcttcc aggtgctctg cgaggccggg ctcctccccg gcgcgctgct
    17221 cgacaacgcc ggcctgaagt tctcgtgccg ccaggtcgac gtgatcgtcg agaccgacgc
    17281 cacgcccttc acgcgcgcgt gcgcgcgagg cgagcgcctg tcgatcccca ccaagcacat
    17341 gtccggccgc tactacgcgc ccgaggagca gctcgcgcag atggaggccg agggccaggt
    17401 cgtcttccgc tacgcggccg gccagaaccc caacggatcc gtgcgcgaca tcgcgggcgt
    17461 gcgcaaccag atcggcaacg tcgtcggcct gatgccccac cccgagcacg ccgtcgaccc
    17521 ggtgctcggc tcggccgacg gcggcaagct gttcgcctcg gcggcgggtg cgacgctccc
    17581 gccgggccgg gagcccgttc ccgcgtgaca cccccgccgg ccaccgccga gccgcgccac
    17641 cgccagctcg gcctgaccga cgccgagtac gagctgatcg tcgagaagct cggtcgcgag
    17701 ccgaacgagg tcgagctcgc cgtgttcagc ctgatgtggt ccgagcactg cgcctacaag
    17761 cactccaaga agctgctggg cacgctgccg accgagggcc ccgcggtcgt gatgggcccg
    17821 ggcgagaacg ccggtgcggt cgacgtgggc gacgggatgg ccgtcgcctt caaggtcgaa
    17881 tcccacaacc acccgagcgc cgtcgagccg ttccagggcg cggccaccgg cgtcggcggg
    17941 atcctgcgcg acgtcttcgc catcggcgcc cgcccgatcg cgatcctcga ctcgctgcgc
    18001 ttcggcgagc tcgactcggc ccgctcgcgc ttcctgctcg atcgcgcggt cgccggcatc
    18061 ggccactacg ggaactcgat cggcgtcggg accgtgggcg gcgagatcta cttcgagggc
    18121 ccgtacgagc acaactgcct cgtcaacgcg atgtgcctgg gcatcgcgcc ccaggagcgc
    18181 ctcacgcgca gcgcggccgc gggcgtcggc aaccacctgg tgctgctcgg cgcgctgacc
    18241 ggccgcgacg gcatcggcgg tgcctcggtg ctggcgagcg cggagctcga cgaggaggac
    18301 gagtcgaagc gcccgagcgt gcagatcggc gacccgttcg aggagaagaa gctgctcgag
    18361 tgctgcctcg agctgctcga cgaagggctg ctcgagtcgc ttcaggacct cggcgcggcc
    18421 gggctgtcct cgagctcgtc cgagatggcg tcgaagggcg aggtcgggat cgacatcgac
    18481 gtcgcgcgcg tgccgctgcg cgaggcggac atggagccgt tcgaggtgat ggtgtccgag
    18541 tcgcaggagc ggatgctctg cgtggtcgcg cccgagcgcc tcgacgccgt gctcgagctc
    18601 tgcgcgaagt gggaggtgcg cgccaccgcc atcggcgccg tcaccgagaa ccgccagctg
    18661 cgcgtgctcg acggcggcga ggtcgtcggc gacatgccgg tcgaggcgct cgtggacgac
    18721 tgcccgctct acgacctcga gcctcaggct ccaaccgagc cggtctaccc ggcgcctccc
    18781 gcgcgcgtcg cgcgcgagac cgcatccgac gatctgctgc tcgcgctcgt cggctcgccc
    18841 accgtcgcca gcaagcgctg ggtgttcgag cagtacgact cgctggtcgg ctcgcgcacg
    18901 gccaggcgcc cgcaggcggc cgacgccgcc gtgctgctcc tcaagcagga gggcgagggc
    18961 ggcgcgctgg cggtgtcgat cgacggcaac ggccgccgcg tcgcgtgcga gccctacgcc
    19021 ggcacggtcg aggcggtgct cgagtgcgcg gccaacctcg cctgcgtcgg cgccgagccg
    19081 ctcgggctca ccaactgcct gaacttcggc aaccccgaga agccgcacat cgcgtggcag
    19141 ctctcggagt cggtgcgcgg cctcgccgac gcctgccgcg cgctggaggt ccccgtcgtg
    19201 ggtggcaacg tctcgctcta caacgagggc gccgagggcc cgatctaccc gacgccggtg
    19261 gtcgggatgg tcgggacgct gccggacccg cggcgcgccg ccgggatcgg cttcgcggag
    19321 gccggccatg cgatcgccct ggtcggcgcg ttcgccccgg cgctggcggg ctccgaggcg
    19381 gcgaagctgc tgggcgaccc gctcgcgccg ctccgcgccg tggacacgac cgcccacgcg
    19441 cgcacgctcg acttcgttcg cgaggcggtc cgcggcggcc ggctggcctc ggcgcacgac
    19501 gtggccgagg gcggcctcgc cgtggcgctg gccgaatgct gcatcgcggg cggggtgggg
    19561 gcacgcgcca attgggcctc cgcgctgcag gcggagcccg gcgaggcgcg cctcgcgatg
    19621 ttcggcgagg gaccgggcgg attcgtcgtc tcggcgccga ctgaggcgct cgcggagctg
    19681 gccgccgagg ccggctcggt gcccgtcgtg accatcggcg aggtgggtgg ggacctgctg
    19741 gagctgaccg cttccgctgc taggctcagc cttccggtcg aggccctgcg atcggcgtac
    19801 gagcaggcga ttcccgacct tctcacttga ccccaaccga ccctctgtca atgcgggacg
    19861 ggccgcgcga cgagtgtggc gtcttcggcg tgtacgcgcc ggagtccgat gttgcccgac
    19921 tgtcctactt cgcgctctac gcgctccagc accgcggcca ggagtcggcg ggcatcgcga
    19981 cggcggaggg cggcgagatc atcgcggtgc gcgagctcgg cctcgtctcc caggtgttcg
    20041 acgagaagac cctccaggcg ctcccgggcg gcctcgcgat cggccacgtg cgctactcga
    20101 cgaccggctc atcggcgtgg gccaacgccc agccgatcca ccgctccgac caccacaacg
    20161 tcgccctcgc gcacaacggc aacctgatca acgccgtcga gctgcacgcc gagctgcgcg
    20221 agcgtggggt cggtttcagc tccacctcgg actccgagat catcgccgcg ctgatctcca
    20281 cccacccggc cgactcggtg ggagaggcga tcgaggacat cctcccccgc ctcgagggcg
    20341 cgttctccac ggtcgtgatg acccccgacg gcgtgtacgc gttccgcgac ggcgccgggc
    20401 tgcgcccgct cgcactcggg cggatcggcg accgctactg cgtggcctcc gagagctgcg
    20461 cgttcgacat catcggcgcc gagttcctcc gcgaggtgct gcccggcgag ctcgtgtcgc
    20521 tcggcgagcg cgggatcgac acgcgccagg tgatcgagtc cgaccggcgt tcgttctgcg
    20581 tgttcgagca catctacttc gcccgccccg acagccagct cgagggtcgg cgcgtgcagg
    20641 tctcgcgcag caagatgggc gagatcctct ggcgcgacgc gccggtcgac gccgacctcg
    20701 tgctcgcggt gcccgactcc ggcaaccccg cggccaccgg ctacgcgctc gcctccggga
    20761 tcccgaagga cgacgggctg atcaagaacc gctacgtggc gcgcacgttc atccagcccg
    20821 ggcaggagct gcgcaagcac ggcctgcgga tgaagttcaa cccgctgccg gaggtggtcg
    20881 gcggcaagcg ggtcgtggtc gtggacgact cgatcgtgcg cggcaacacc acccgccaga
    20941 tcgtcgggat gctgaaggac gcgggcgcca ccgaggtgca tctgcggatc tccgcgccgc
    21001 ccacgcggca cccgtgccac tacgggatcg acatgtccac gcggcaggag atgatcgccc
    21061 acgagcgcac ggtcgaggag atcgcgcgcg agctgggagc cgattccctc gcgtacctca
    21121 cgatcgaggg cgtgtacgag gcgatcgaat ccacgcgcga gacccactgc gacgcctgct
    21181 tcaccggcga ctacccgctc gagcgctccg agggcgcgcg cgggaagtac gcgctggagg
    21241 agctggcggt agtgcccggc tagcggcgct gcgccggtag gctccgcggt gtggcgcttc
    21301 cgcccggtcc gcggttcgca ccgctccaag cggtgcgctt cgtgcgcaac ccgatcgatt
    21361 acctgacgga tctccagcgc cgccatggcg acgccttcac cgtgccgttc ccgttcctcg
    21421 gccggctcgt gtacttcgcc cacccggacg cgatccgggc gatcttcacc ggtgacccga
    21481 agctgttcca ctcgggtgag gcggtcgagc cgatcctcgg cccggtcgtg ggcgcgcact
    21541 cggtgttcgt gctcgacgag gaagcccaca tgcgcgagcg caagctgctg ctgccgccct
    21601 tccacggcga gcgcgtccgc cgctacgcgc agacgttcgc ggagatcacg gagcgagagg
    21661 tggagcgctg gccgctcggc gcgccgttcg cgctgcgtga gcggatgcag tccctcacgc
    21721 tcgaggcgat cctgcgcacg gtcttcggga tccacgatcc cgcggtgatc gctcgcttcc
    21781 aggagcgcgt gccgcgcatg ggacgggtgg gcaacctcgt ggtgatgctc cccgggctgc
    21841 ggtgggacgg tgaccgcttc ggtccgtggg cgcggttcgt gcgcgcgcgc gaggcggtct
    21901 acgagctgat ctacgcggag atcgaccgcg cggcggccga cccggcgctc gacgagcgcg
    21961 acgacgtgct cgcgctcctg ctccgcgccc gccgcgagga cggcagccgg atgggccggg
    22021 acgagctccg cgacgagctg atgtcggtca tcgccgccgg ccacgagacc acggccaccg
    22081 gcctcgcatg gttcttcgag cgcgtgctgc gccatcccga ggtcgaggag cggctgcggg
    22141 ccgagatcgc agccggggat ggggacgagt acctcgacgc gacggtgcgc gagaccctgc
    22201 gcgtccggcc ggtgatcgtg gacgtcgtgc ggcggctgaa acgccggacg acgatcggcg
    22261 gctgggacat cccggcgggg acggacgtgg tcgccgccgc ggccgtggtg cagctgcgcg
    22321 aggacctata ccccgagccg cgcgcgttcc gcccggagcg ctttctcgac ggtggtggcg
    22381 ccgacggcgc ctacgcctgg atcccgttcg ggggcggggt gcgccggtgc atcggcgcct
    22441 cgttcgcgca gctcgagctg aaggtgatcg cccgcacgat cctcgagcac gcgcaactca
    22501 gcgccgccga tgcgcggccc gagccgccgc gcgcggcaca cgtgacggtg gtccccgcgc
    22561 ggggcgcgcg cgtggtgctg gagcagcgca tcgcgccgcg ggcagaggcc gtggccctcg
    22621 cctccgcgtg atggcgcgcc cggagccgcc gcccggccca aggttcgcgc cgctccaggc
    22681 gctgcatctg ctgcgcgagc ccgtgacgta cttcgagcgg ctccagcgcc gcttcggcga
    22741 cgccttcacg gtgccgatcc cgctgttcgg caaggtcgcc tactttgccg accccgaggt
    22801 ggtccgggag ctgttccgtg cggacccggc cgtgttccac gcgggcgagg cgaacgcgga
    22861 cgcgctcggc cccgtggtgg gcgcgaactc ggtcatcacc ctcgacgacg acgcgcacct
    22921 gcgcgagcgc aagctgctgc tgccgccgtt ccacggcgat cggatcaacc gctacgcgga
    22981 gctgttcgcg gagatcgcgg cacgggaggt cgagcgctgg ccggtggggg aggagttcgc
    23041 gctgcgcccg cggatgcagg cgctctcgct cgaggcgctc ctgcggacgg tctacggcgt
    23101 ggacgacgac gagcgcgtgc gcgagtacca ccgggccgtg gcgcacatga tccacgtgag
    23161 caacatcgcg gtctgggtgc cggcgctgcg ccgcgatgcc ggccggctga gcccgtgggg
    23221 gcgcttcctg cgagcgcgtg cggcgttcga ccggctcgtg cacgaggaca tcgaccgcgg
    23281 gcgggccgat ccggcggcgc tcgccgagcg cgacgacgtg ctctcgatgc tgctccaagc
    23341 ccagcgcgag gacggcagcc tgctcacgcg cgaggagctg cgggacgagc tgacgaccgt
    23401 gatcgtcgcg ggccacgaga cgaccgccac ggggctgagc tggttcttcg agcgcgtgct
    23461 gcgccacccg gaggtcgagg agcgcctgcg ctcgggcgac gacgcctacc tcgaggccac
    23521 ggtccaggag gtgctccgca cccgcccgcc gttcttcgac gtcgtccggc gcctcacgcg
    23581 cgacgccgag gtcggcggct atcggatccc ggccgggatg atggccgggc tctcgatcgt
    23641 gctcgtccac cagcggccgg acgtctatcc ggagccgcgc gccttccgcc ccgagcgctt
    23701 cctcgagacc gcgccgggcg gattcacctg gatcccgttc ggcggcggcg tgcaccgctg
    23761 catcggggcg tcgttctcgc tgctcgagac acggacgatc gcccgggcga tcctcgagcg
    23821 ggtgcgcctg cgccccgccg atcccgcgcc ggagaccccg cgcacgaaca acgtgatgat
    23881 ggtgcccgag cacggcaccc gcgtggtgct ggaggagcgt tgcgttcccg caacgtccgt
    23941 tacaactccg caacggagcg ctacggcagc gtaaaaatgc gcccgctccc gccgagcggg
    24001 ccgataccgt ggctgcatgg acttggacgg cggcctcaac gagtggttcg gctttcgcga
    24061 cttccggccc gggcagcgcg aggcctgtgt cgcggcggcc gaggggcgcg acgtcctggt
    24121 cgtgatgcca accggctcgg gcaagtcgct ctgctaccag ctgcccgccc tgctgcgcga
    24181 cgacctgacg atcgtggtct cgccgcttgt ggcgctgatg caggaccagg tggacgcgct
    24241 gcgcgcgcgc ggcctgggcg accgcgtggc ggtgatcaac gcgcagcagg acgcctcggc
    24301 gaacgccgag gtgctcgacg gcgcggcccg cggtgagctg cgcctgctct acgtggcgcc
    24361 cgagcgcttc tcgtcgcccg gcttcttcga gcggctgagc cgcgcgcgga tcgggctctt
    24421 cgtggtggac gaggcgcact gcgtctcgca gtgggggcac gacttccggc ccgactactt
    24481 ccggctcggt gatgcggcgc gcaagctcgg cgccgccggg atcgtcgcct cgacggccac
    24541 ggcaacgccg caggtggcgg ccgacgtggt gcggcggctc gcgctgcgcg agccgatccg
    24601 cgtcaccacg ggcttcgacc ggcccaacct cgcgttcgcc gtggcacggc cggcgccgca
    24661 ccagaagcgc gcgctcgtcg tggaggcgct gaagcgcgag ggtgcgctcc cggcgatcgt
    24721 ctacgcgggc acgcgcgcgg ggtcgaacga gctcgccgag gtgctcacgc gcgagctggg
    24781 ggaggaggca ctgccctacc acgccggtct cgagcgcgac cgccgcgcgg cgatccagcg
    24841 tcgcttcctg accgacgacg tgcgggtggt cgtggccacc aacgccttcg ggatgggcgt
    24901 ggacaagccg aacgtgcgaa cggtgctcca ctccagcgtg ccgtcgtcgc tcgaggccta
    24961 ctaccaggag gcgggccgcg ccggccgcga cggcaagccc gcgcgggcgc tgctgctcgc
    25021 cgagaaccgc gacaaggcgc tgcatgtgca cttcatccgc cgcgacgagc tgcgcgagga
    25081 ccagcccgag aagctcgccg ccgtcctcga gcgcgacgcg ctctccgacg gcgcgctgcc
    25141 gggcatgtcg gagccgcgct acgacgtcga cgcgcgcgag ctggcaaacg ccgcgggctg
    25201 ccgcggcgat cacgtggcgg cgctggtggg ccaccttgcg cgcgcggggg tggtgtcgcc
    25261 cacgccggcg ccgccggacc gggtcgccgg ccgcctcctg cgctcggtcg gcgggcgcga
    25321 acgggcgctg tgccgctcgt cgatcgccga cgccgcccgc gctcgctggc gccagtaccg
    25381 cgaggtgtgg gcctacgtgg agagcgagag ctgccggcgc cgcaccatcc tccgccactt
    25441 cggcgaccac gccgagccgg ccgtgcatca cgacgccgca ggcaacgccc tgccgtgctg
    25501 cgacgcgtgc ggggccgccg acctcctgcc ggagctcccg cgggtggaca cgcaggaggc
    25561 ggtcgaatcc ctcgacgacg cgatcttctc ggtcgccagg acggccaagc ccgaggtggg
    25621 caggaccacc tgcgcggaga tcatccacgg cgcgaagacc aagaagatcg agcgcaacag
    25681 ctacgacggc ctcgccgcgt acgccaccgc gtcgcacatg cgccgcgcgg acatcctcgc
    25741 gcggatcgac gagctgatcg gcgaggagcg gctcgccacg agcggcgggc cctacccggt
    25801 gctcaaggtc gggccgcagg ccgccgcggt ggcgtgagcc tgcgcgtggc agtgctcgtc
    25861 tcgggcgagg gctcgaacct ccaggccctg atcgactccg tgcacgccga gggagccgtg
    25921 gagatcgtcg gcgtcgcctc gaacaaccca ggcgcgcacg ggctccggcg cgcgcaggcg
    25981 gccggggtcg agaccgccgc gttcccggcg tccgagtacc ccgaccggac cgaccgcgac
    26041 gatgccctgg ccgcgtggct cgaggagcgc gacacgggcc tcgtggtgct cgcgggcttc
    26101 atggagctcc tcggcggcga gttcgtgcgg cgcttcagcg gccggatcgt gaacgtccat
    26161 ccgtcgctcc tgccggcctt ccccggtgtg cgggcgatcg agcaggcgct cgactacggg
    26221 gtgcgggtca tgggggtcac ggtgcacttc gtggacgagg gcgtcgacag cggcccgatc
    26281 ctcctgcagg aggccttcga tccggtcccg tatgctcgtg acatcgacgc cgtggagaga
    26341 cgcgtgcacg agatcgagca caggctcctg ccggaggcgg tgcgcctgat cgcggcgggc
    26401 cgcgtccaca tcgatccaga ggatccgcgt cgcgtgaggg tcgatgggaa cgactgaggg
    26461 cgccgccccg caggtcacgc agccgggcgc agtgcgggtc cgccgcgcgc tgctgacggt
    26521 ctccgacaag cgcggcctcg tcgacttcgc gcgcggcctg gcggagctcg acgtcgagat
    26581 cgtctcgacc ggtggcacgg cgcgcgagct cgagagcgcg ggcatcgaga cccgcccgat
    26641 cgacgactac accggcttcc cggagatcct cgacggtcgg gtgaagacgc tcaacccgcg
    26701 gatctacgcc ggcctcctgg cagtccgctc gaagcccgag cacgtggcca cgctcggcga
    26761 gcaggagatc gagccgatcg acctggtctg cgtgaacctc tacccgttcg agcgcacggc
    26821 ggcggcgcgt ggggcctcgg tcgaggagat cgtcgagaac atcgacatcg gtgggcccac
    26881 gctgatccgc gcggccgcca agaaccacgg gttcgccgcg gtcgtgacga gcccggagag
    26941 ctacgacgcg gtgctcgagg agctgcggat gagcgacggc ctgctctccg agcagacgcg
    27001 cgaggcgctc gcgatggacg cgttcggata cacggcgcgc tacgacgccg cgatcgcgcg
    27061 ctggttcgcc gagcgcgagg acgacttccc cgcgatctac ccgcgcgtgt acgagaaggt
    27121 gctcgacctc ccatacggag agaacccgca ccagcgcgcg gcctactacg cgcaggcgaa
    27181 cgcgcgcacg cacctgatgt cgatggtgtc gaagctccac ggcaaggagc tgtcgttcaa
    27241 caacttgctc gacctcgact ccgcccgccg cctggcgcac gagttcgagg tgccggccgc
    27301 ggtgatcgtc aagcacaaca acccgtgcgg ctgcgcggtg ggcagcacgc cgctcgaggc
    27361 gttcgagaag gcgctggcct gcgacccggt gagcgccttc ggcggcgtgt tctgcttcaa
    27421 ccggccgatc gagcgcgacc tggccgagcg cctcaacgag atgttcgtcg aggtcgtgat
    27481 gacgaagggc tacgaggagg gcgccctcga cgtgctctgc cagaagccga acccgcggat
    27541 cctccacgac gccgagcgcc gccgcgagcc gacggccgac cgcgacatgc ggcgcgtccg
    27601 cggcggcctg ctggtgcagg accgcgacga ggacgtggag ctgcgcgacg agatgcaggt
    27661 ggtcacggag cgcaagccga ccgagcagga gtggggcgag atgctgttcg cctggaaggt
    27721 ctgcaagcac gtgcgctcga acgcgatcgt gatcgccaag gacctcgcgt cggtcgggat
    27781 cggcgcgggc cagatgagcc gcgtcgactc ggtgcggatc gcgatcgaca aggcgcagtg
    27841 cccgctcgag ggcgccgcgc tcgcctcgga cgccttcttc cccttcgcgg acgggcccca
    27901 ggcggcgatc gaggccggcg tacgcgcgat catccagccg ggcggctcga agcgcgacga
    27961 cgaggtcatc ggcgcctgca acgacgccgg tgtggcgatg gtgttcgccg cccgccgcca
    28021 cttccggcac tagcccgacc cacgcctggc ctcaaccgcc cgcgcagcca caactaggct
    28081 tcgcgcgggg gcgtagctcg aaggtcgagc agcggccttt taagccgacg gcctgggttc
    28141 gattcccagc gcccccacgt ctttgccgcc gcttaacacg cgcccggttg cggctcgggc
    28201 gggtcgcacc taccctgatc gtggacccgc aacttctttg ggggacgagg gttaaggtgc
    28261 caggatcggg cgccgtgacg ctcggacacc gagggggagg actcgcaagt tgaccgctcg
    28321 acgctgcaac cgctccgcgg ccaggaacgc cctggtcgcc atcatcgcgc tcgcgatcgc
    28381 tctgctggcg ccgagtgcgg cgagcgcgag catggtcgcc ctcaacggca cgaccctggc
    28441 ctacggcggc gacgcggttc cgaataccgt cacgatctcg ctttcgggcc tgagctacgt
    28501 catcgacgac tcggtggcga tcaccccgac cgccccctgc cagaacgacc ccaccgacga
    28561 cacgatcgcc gagtgccccg cgctgacggt cagctcgatc agcgcaacgc tgagcccgac
    28621 gccgaacgcg ctcgacggga acaacgccct cgtccttgac agctcgatcg gcgacaccac
    28681 gaccgcgacc gtcgcgggcg gctccgccgt ggacaacttc accggccagg aggcggccga
    28741 gaccttcaac ggcggcagcg gcaacgacat cgccaacggc ggggacggca acgacccgct
    28801 gaacggcgac gccggcaacg acacgctcaa cggccagggc ggcaacgaca ccgccctcag
    28861 cggcggcgcc gactccgacg ggatcgacgg cggcaccggc aacgacgccc tcaacggcgg
    28921 cggccagggc gacgacctgg tcggcgggga cggcgacgac actctgcgcg gcaacaacct
    28981 cctgaacacc gacgccgccg gcgacggcag cgacgagctc cacggcaacg gcgacaacga
    29041 catcctcgtc ggccactcgg ggcccgacga cctcgacggc ggcgacggcg tcgaccgcgc
    29101 cgactacggg gaccgcggcg cgaccgacgg cgtcaccgtc acgctcgaca acgacaccgc
    29161 ccaaaacgac ggcggcgcgc tggatggcac cgagggcaac cgcgacatcg tccgcaccaa
    29221 cgtcgagaac gtcttcggcg gagcgggcgc ggacaccatc accgacgcgg tgaccgccag
    29281 caccgtcgac aacttcttcg tcggcgacgc cagcggggac tccctctacg gcggcctcgg
    29341 cgccgaccag ctcgagggcg acggcggcga tgacgtcctg aacggcgagc gccccggaat
    29401 caccgtcgag agcgacggcg acaagttctt cggcggcgcg aacaccgaca cggtggacta
    29461 ctcgagccgc acccaggacc tccggatcga cctcgagaac gaggatctcg acgacggcac
    29521 ggtcgtcgcc ggcgtcagcc aggaaaacga cagcgtcttc gacgacgtcg agaacgtctc
    29581 gaccggcggc ggtaacgaca cggtgacggc gcaggccgcc gacgtggcga acaccttccg
    29641 cggcggcgcc ggggccgaca cgctcagcgg cgacgctggc gcggacagtc tcctcggcga
    29701 ggcgggcaac gacctcggcc tgaacggcaa cgacggcgcc gacaccctct cgggcggcga
    29761 cggcgccgat cagggactcg cgggcgacgc gggcaacgac acgatcgacg gcggcgaggg
    29821 cgcggacgcc ggcaacggcg gcgcggacgt ggacacgatc tcgggcggcg cgggcaacga
    29881 cagcctctcg ggcggtggcc agagcgacac gctcaacggc ggcgccgacg aggacacgct
    29941 gaatggcgac gccggtgccg acgccctcaa cggcgggacg cagaacgaca ccctcaacgg
    30001 cggcgccgac ggggacacgc tccatggcga cgacggcgac gacggcctca acggcgaggc
    30061 cgccggggcc aacgccacgc cgagcaacgg cgacgacatc catggcgacg cggggaccga
    30121 cacggtcacc tacaaccgtg cctcgtccgg cgtgacggtc gacctcgacg gcgacgacaa
    30181 cgacgatggc ctccagggcg ggagccctga gggagacagc gtcttcgaca ccgtcgagaa
    30241 catcgtcggc accgacgaga acgacaacct caccggctcc ggcagtgcga accgcctcga
    30301 gggcgggccc ggcgacgaca ctctcgtggg tggggccggc gccgacgatc tgctcggtgg
    30361 atccgacagc ggcggcgtct ccggaggcga tgtgctgcgc ggcggcccgg gtcccgacga
    30421 cctgagcggc ggggcgggcg acaacgacgt ggcgcagtac gacgagcgca ccagtggcgt
    30481 caccatcacg atcggcggga cacccggcga cgcgaccgtg ccgcgtgacg acggcaacgc
    30541 cgacgacgga gccgccggcg cgcgcgaccg tgtcctcgag gacatcgagg acgtggttgg
    30601 cggcagcggc gccgacgaca tcaccggctc caacgtgggc aacgtcctga ctggcggcct
    30661 cggcaacgac gacctcgacg gcggcctcgg caccgacacg ctcaacggcg ggggcgacaa
    30721 cgacaacctc aacggcggcc cgggcggggc ctctgatttg ggcgacacgc tcaacggcgg
    30781 cgacgaggag gacacggccg actactcgac ccacaccgcc gacgtgacgc tcacgctcga
    30841 cggggccgct tccgacggcg cgacgggcga gctcgacacg atcgcggccg acgtggagaa
    30901 cgcgcgtggc ggttccggca acgacaccgt ggccggcaac cagggcgaga acgagttgga
    30961 cggccacacg ggcgacgaca cggtgaacgg cggcggcggg gcggacctcg tgcgcggcgg
    31021 gccggacaac accgcctcac cggacgccgg cgacaccgtc aacggcgagg gcggcaacga
    31081 cgtgctcgag ggcggcgacg acgccgacga cctgaacggc ggcagcggtg acgaccagct
    31141 cgacggcggc accggccccg accgcatcga cggcgggctc gcaaccgaca cggtcaccta
    31201 cggggaccgc acggccgccg tgaccgtaga catcgacggg accgtgggcg acgtgaacgc
    31261 gccggccgac gacggcaacg cggacgacgg cgcgaccgcg accacgcgcg acaccgtcta
    31321 cgactccaac gagaacctgg tcggcggatc gggcggcgac acgctgaccg gcgacggcga
    31381 cgtgaacacg atcgacggcg gcgcgggcac ggacacgatc accggagggg ccggggttga
    31441 cgtgatgctc ggcggcgacg acaacgacga gatcctcgcg gtcgacgcaa cggtcgagac
    31501 cgagatcgac tgcggcgccg gcacgggcga catcgctcgc atcgacgact cgccgggcat
    31561 cgtcgacacc acgcagaact gcgagcggat cgacgacgcg aacgacgacg acgacgcctt
    31621 cccggacgcg accgacaact gcgacaccac gaagaacgac gaccaggcga acttcaacgg
    31681 ggacgccgag ggcgacgtct gtgacgacac cgacagcgac acggtcttcg acgcgaccga
    31741 caactgccgg accgacgcca acacgaacca gacgaacacc gacggcgacg cgcagggcaa
    31801 cgcctgcgac gccgacgacg acaacgacgg cgtgaccgac gcgcaggagc agcagcgcgg
    31861 taccaacccg ctggtggtcg acagcgacgg cgaccagtcg aacgacaacg ccgacaactg
    31921 cccgacgacc ccgggcagcc aggccgacaa cgacaacgac gggcagggcg acgtctgcga
    31981 cgccgacgac gacaacgacg gcaaggccga cggctccgac aactgcccag cgacggccaa
    32041 cgccgaacag gccaacgccg acggcgacgc ccagggcgat gcatgcgact ccgacgacga
    32101 caacgacggc gtcgacgact cgaaggacaa ctgccgcatc gtgggcaacg cgaaccaggc
    32161 cgacgcgaac gggaacggag cgggcgatgc ctgcgacgac gaccgcgacg gcgacacgat
    32221 cgccaacggc aacgacaact gcccgacggt ggccaacacc gaccaggcca acagcgacgc
    32281 cgatccggag ggcaacgcgt gtgaccccga cgacgacaac gacggcgcac tcgacgagtc
    32341 cgacgacttc ccgaccgacc cggcgcgcca gacgaaggtc ccgctcggga cgaacggcga
    32401 cgacctgatc aacgggtcct cgctcgacga cacgatctgc ggcctcggcg gcgacgacat
    32461 catcaacggc ctccagggca acgacacgct cttcggcgac gcatgtgacg cgcgccagaa
    32521 gatcggctcc gcgcaggtcg cgacggcggg cgacgacaag ctcaccggcg ccgagggcaa
    32581 cgacctcctc ttcggcggcg gcgcgaacga caagctgagc ggcggcctcg gcaacgacag
    32641 cctgttcggc ggcgcgggca acgaccgcct gagcggcgac gacggggccg acatcctgct
    32701 cggcgacgac ggcaaggacg tgctctcagg cggctccggc aacgacaacc tccagggcgg
    32761 cgccgggaac gacaacatga acggcggcca gggcaagaac acgttcgtcg gcggctcggg
    32821 caacgacatc atcaacgcgg tgaacggccg caaggacacg atcgactgcg gcaagggccg
    32881 cgacaagctg cgcgcggacc gaaacgacaa gatccggcgc ggctgcgagc ggatcatcaa
    32941 ggcgaagggc tagcctggcc ctccgcccgt gaccctcgta cgactcgcat acctgcgccg
    33001 gcgcacgctg gcggtgctgc tggccttcgc cacgctcgca tgcgtgttcg cgtgcgtggg
    33061 ccaagcccag gcggccaccc gggccagccg cagcgaccgc gacgccgacg ggatgcgcga
    33121 cgcctgggag cgccagcacg gcctcaaccc gcgcagctcc cgcgaccgca agcgcgatcc
    33181 cgaccaggac cacgtgcgca acttcgacga gttccgccag ggctgcgacc cgcaggcggc
    33241 cgacagcgac cgcgacggca tggacgatcg cttcgaggtc cgctacggct tcgactgcgc
    33301 gcgccgcggc gaccagacgc tggacgtcga cggcgacggg ctctccaacc gtcgcgagaa
    33361 cgacctcggc accgatccca ccgcctccga cacggacggc gacgggctct ccgactccga
    33421 cgaggtgcgc gcggggagca acccggtgcg tggcgacagc gaccgcgacg gcgacctcga
    33481 cggcgccgac aactgcctgc tcgtctcgaa cgccgaccag gccgaccgcg acggcgacgg
    33541 ccagggcgac gcctgcacgc ccgacgccga caacgacggc gtcgccgatc cccgcgacaa
    33601 ctgccgcacc acggcgaacc cggaccagcg caacgcggac ggacgcgccg acggcggcga
    33661 cgcgtgcgat cccgacgacg acgacgacgg caccgccgac ggctccgaca actgccccac
    33721 ggcagccggg ccgcagaccg acacggacgg ggacggcaag ggcaatccct gcgacgagga
    33781 cgacgacggc gacgggctga gcgacgccga cgaggccgca cgc
//
LOCUS       DASVPL010000149        12648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_254739, whole genome shotgun sequence.
ACCESSION   DASVPL010000149 DASVPL010000000
VERSION     DASVPL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254739"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF517_07715"
     CDS             complement(<1..317)
                     /locus_tag="VF517_07715"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8052863.1"
                     /translation="MAKRRVHEIAKERGISSKEVIAILQKAGLDVKTAAQSVDEGDIA
                     RAFDPNAGKNGAGAKGDGANGRAAGGQAGAAGDGQSRPQRPTRAAPPNAAGPGGRRRR
                     VVI"
     gene            complement(556..2196)
                     /gene="nusA"
                     /locus_tag="VF517_07720"
     CDS             complement(556..2196)
                     /gene="nusA"
                     /locus_tag="VF517_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX8052864.1"
                     /translation="MSKEIVEAVHVLEREKGISAERLMLALEDALLSAYKKTPESAQY
                     ARVEMDRETADFKVYELIIPPEIEEQVLVEVELEESLVDPETGEQREPPEPELDLEKL
                     EEFRDQIEEHDVTPDDFGRIAAQTAKQVILQRIREAERDMMYEEYRDRVGELITGIVQ
                     QSDSRYTLVQLRERVEALLPKSEQVDNERYDHGQRVKAVITDVSAQAKGPSIIVSRRS
                     PELIRKLFELEVPEIADGLVEVQNVAREPGYRSKIAVVSHASGVDPVGACVGPRGSRV
                     RMVVSELRGEKIDIIPFNEEPARFVAKALSPARVREVLVDDDSKQATVVVPDDQLSLA
                     IGKEGQNARLAARLTGWRVDIRSETEFAAEEADMDYDEEETAGRCAAIMSNGRRCPNA
                     SLPGSRYCGLPAHQELEGREGDEVAPAAEEAAGDAGPSGEAAPEPAAEEAPEGEPAPP
                     PVAEGAPDPAEEQPPAEDEPVAETPDPESSASAAEPDPGSGGAVEQPPAGDDAEADSP
                     DPESSAQAPPPTPDADAGDPTAGADGTPEGAPATEEAS"
     gene            complement(2201..2620)
                     /gene="rimP"
                     /locus_tag="VF517_07725"
     CDS             complement(2201..2620)
                     /gene="rimP"
                     /locus_tag="VF517_07725"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX8052865.1"
                     /translation="MERDIQTEIETRLATAEPDVEVLLAERIAGERVRVFVDHPEGVS
                     LALCERVTLHLRDLLTEYSLEVSSPGPERPLSKPDHYRRFVGRKVRVRTRGEHDGRRS
                     FTGELVGATDDEVTVAADEGVVTIPYEDVKRGNLLEG"
     gene            2762..3577
                     /locus_tag="VF517_07730"
     CDS             2762..3577
                     /locus_tag="VF517_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052866.1"
                     /translation="MRVGLATAIFLALAAAVMWAVPSGAHDSLAPRAAHHHWLPNEMW
                     SHKHWVPYDESRLYALLGVDTRELFEWLADDHHTIAQLARRHGVDPRTLAKRLMEPRR
                     ASLSGAEYRTLLDRAERTITQGHLGQHVLFHVFHGSHLNGHETGHIEQLFGVDRAKWN
                     RLRQRGWSPLRIATHHGRPADAVREHVIQSLREESERGVRDHATTPEQGERMLARQLR
                     VVDCWLSSPLAKNDPYNPFGDTWGWHGPHKRGVRNGVSNAKTPRGCWATLPDA"
     gene            complement(3596..4021)
                     /gene="dtd"
                     /locus_tag="VF517_07735"
     CDS             complement(3596..4021)
                     /gene="dtd"
                     /locus_tag="VF517_07735"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013242072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX8052867.1"
                     /translation="MRAVVQRVSEASVSVDGERVSAIGAGLLVLLGVRVGDTPGDADW
                     LARKIHALRVFEDDEGRMNRSIGDVGGAILCVSNFTLYGDARRGNRPSFAEAAPPSEA
                     EPLYERVRAALGAAGGTFGAHMEIELVNDGPVTLVLDTP"
     gene            4039..4467
                     /locus_tag="VF517_07740"
     CDS             4039..4467
                     /locus_tag="VF517_07740"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX8052868.1"
                     /translation="MAAVDVVNEYMDSLEGETRKLAHAEWGLTLSAESAGGWPCDIGV
                     RIDDGLLRVQAYALEAREDIDPGVLLQWNRQTRLVRMASTRSGDIWVLADLPVKAVDE
                     QQVDRLLGLIAEGVLAVRGYARALTEPPPATTSGWLRAGP"
     gene            complement(4451..4795)
                     /locus_tag="VF517_07745"
     CDS             complement(4451..4795)
                     /locus_tag="VF517_07745"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX8052869.1"
                     /translation="MKKLVLAATAAAVLVLPASASALKANMIVAPNPAVTGQPVIFDG
                     SASIPLTFQISCPSVIETYTWNFGDGSGATGKQVRHVYARGGSYNATLTVDSGAEWCL
                     DDTDAKTVTVLP"
     gene            complement(4836..9374)
                     /locus_tag="VF517_07750"
     CDS             complement(4836..9374)
                     /locus_tag="VF517_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8052870.1"
                     /translation="MTAAMTRVAVAEFPYSESGGDKRDFKTLRVPKGAIPKDLPRKDE
                     NVWKFSATPEENNFPVNLEPRELFGVRGGHVVDADATKETAWTVTTGRPDVKIAVLDS
                     GFRWNANQVLDLRFKTFLNRGELPQPNHSGPDLVGGVNCGSYANAHDANGDNVFNLLD
                     YACDSRVRKDTPKNVRTDVLDPQDLLIAFSDGSDADGNGFADDMVGWDFLDDDNDPFD
                     DVQYDHGTGEAEGSSAEANNDAEWVGTCPNCMAMHMRVGDSFVADDNDFAQATLYAVD
                     NGALVIQEALGTLNNTQLAQRAIDYAYNHGVIVIASAADEAAQHHNYPSSHEHTLVVN
                     SVTKYMSQETPDGPPRADVPIQQPRSYVSFNGCTNFSSKIGLAIPSTSCSSDAVGVGA
                     GMAGLVLSAALNGVENGKLRPHPTCRRADGSPCPLSANEVRQVMASGTFAGQTQPDDV
                     NFNQSVLGPQPEPSCSPEPAPGCTDPNLYTQQQVNGNRPIVSPPDSRSYPARAGHDQF
                     YGWGRINTFRSVRRAADGQTAPEVEITSPEWYAQVDPAKPTAQVDAQVWARGAKYSCK
                     VYVAPGSYPNNHFTTDSPPGDFAQVPSPVCDGQARTEPIDGVVAALDVNALKERFPAD
                     AGDFRGPEPGHGAGQTSNGRPNAEPYGFTVRVIAESAEQNPGLTATGEDRRNLFLHRD
                     QDMLDGFPKRLPSDGESSPAFADLDGDNRNELVFGTADGWVHAIRPDGSEAKGWPVRT
                     DPLPLHAGGRAFASGEVPASASHGAVLSSPAVADLDRDGSYEVVIADFEGKVYAWSAA
                     GRLVWRKSANPEFSGKPLAPFENARVGERNRTRLGFLASPVAADLDRDDGGRLEVVLA
                     AMDRHVYALNDDGSGVPGYPILVVDLRKVKSIDPRTHQVTFDDKKTGGSLQQGAIVDT
                     PAVGNIAGDARPEIVVGTNEEYAANQGDEGPANISGNNAATAQAVSRLGQELVGANTR
                     LFAIKPEGDRDGKPATGDWVAPGWPAKMALLLPELLPVVGEGVTGNPAIGPVNCPSGG
                     AGSKVGAAGVTGVAYVFNGDGTSCVGRQDGRDTSMDTDGGNGSMADRPAFPALGHPAF
                     GDIGGGVSLLSPVAGLTRALDLLLTEYQNTGQDGIAVWDPASGRFRQGFPARMNDLQF
                     LTGPSVANVDAGASEEVVAASAHLDLQAYSGSGQPVARWPKLTSDWVVANPLIGTFGT
                     LDSDPAARRVVVSNTRGGSVLAYRTDAPACQSGARPLGSWPRFHHDLANSGDYSRDAS
                     NPGAAGDVKLYTAGISLRAPGDDLMCGKVAGYELVQPDRALTGATFEEGDPIPVVKRA
                     QDLAVPGALEGIGLGGRLSRYLSIRAVDDQGNVGPATTISTRDVLPGTPPELAGIAGQ
                     IDSCADRSAPRSSISKKGLTLNRKRVAVSGRSADRSCPGREAVATTVAISKREGKRCR
                     FVKSDGRLSARRKCGQPIKLRTKAKFDKKTRKVSWSLKKALKIPRGRYRLRVTAADQS
                     GNLERRTSRKNARSFSVR"
     gene            complement(9439..9828)
                     /locus_tag="VF517_07755"
     CDS             complement(9439..9828)
                     /locus_tag="VF517_07755"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8052871.1"
                     /translation="MADRHDSPEARGEHVYDLFQRGSALLEAGDFSAAAIPLERARAL
                     EPDKSSIREALGRAYFRSGRYADAGEEFAAVVERYPVNDFAHFCLGRSLEKSGRRDEA
                     RRHLALAAQLRPDRADYRVYRDRLRAA"
     gene            complement(9821..11083)
                     /gene="coaBC"
                     /locus_tag="VF517_07760"
     CDS             complement(9821..11083)
                     /gene="coaBC"
                     /locus_tag="VF517_07760"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX8052872.1"
                     /translation="MARILLGVTGGISAYKAVELVRCATRAGHSVRVVQTPSSERFVG
                     RATFEGITGAPVLVDEFATDPARGAFPGDPAPDHAPIAHLELVRRADVYAIVPASANT
                     IAKLATGLADNLLTSAALASTAPLVLAPAMNDRMWSHAATQANVDTLRERGATVVPPA
                     TGALASRGEWGTGRLAEPPEVLAAIEEVLRAGPYAPRSLDGLRLLVTAGGTREPIDSV
                     RFVGNRSSGRMGFAVAEEAARRGAEVTVVAANVALPRKEGVRYVDVETAAQLREATHA
                     EFAGADVLVMAAAVADFRPADPADTKLGKRDRVGLTLELEPTEDILSELAGQRTGTQV
                     VVGFAAEHGDGAVERARGKLERKRLDAIVVNDISRPDIGFDTAENEVAIVTPAGVREV
                     PFASKAEVAASILDVVEELRTAARVAHG"
     gene            complement(11097..11327)
                     /gene="rpoZ"
                     /locus_tag="VF517_07765"
     CDS             complement(11097..11327)
                     /gene="rpoZ"
                     /locus_tag="VF517_07765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX8052873.1"
                     /translation="MIKPRLDTLLERVDSHYACVLVAAKRARQINSYYHNLGEGTFDE
                     YPPPMVDTGSKNYLKISLEELAAGKINYRYRT"
     gene            complement(11324..11857)
                     /gene="gmk"
                     /locus_tag="VF517_07770"
     CDS             complement(11324..11857)
                     /gene="gmk"
                     /locus_tag="VF517_07770"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX8052874.1"
                     /translation="MLVVTGPSGVGKGTLIKALCREIPDVRLAVSATTRPPRSGEENG
                     VDYHFLTHEDFDRRVAAGEFLEHATYAGNRYGTLRSEVEDDPVVLEIEVQGARQVRAA
                     LPEAIQVFIAPPSTDVLRERLLARGTDSPEVIRARLQVAEEELAAQHEFPHVIVNDEL
                     EPAVRELTGLVATMWRP"
     gene            complement(11866..12213)
                     /gene="mihF"
                     /locus_tag="VF517_07775"
     CDS             complement(11866..12213)
                     /gene="mihF"
                     /locus_tag="VF517_07775"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX8052875.1"
                     /translation="MSPAAPPQTKTQVGAAPERSLTQRMDALRRANEIRTRRAELKRD
                     LKGGRAQIHGLLLDPPEYLQTAKVFDLLLAVPKYGRVKVNRILTQCRISPSKTIGGLS
                     QRQRTELVSYLRR"
BASE COUNT         1610 a         4472 c         4605 g         1961 t
ORIGIN      
        1 tcgatgacca cgcggcggcg gcggccgccg gggcccgcgg cgttgggcgg agctgcgcgc
       61 gtggggcgct gggggcgcga ctgaccgtcg cccgccgcgc cggcctggcc gccggccgcg
      121 cggccgttcg cgccgtcgcc cttcgcgccg gcgccgttct tcccggcgtt cggatcgaag
      181 gcccgggcga tgtcgccctc gtccacgctc tgggcggccg tcttcacgtc gagaccggcc
      241 ttctggagga tcgcgatgac ctccttgctg gagatccctc gctccttggc tatctcgtgg
      301 actcgtcttt tcgccatgcg cgcaataagt ctagggggtc gtcaggtggt gttacgggcg
      361 ccctgaacga gcggtcgaag cccctccggg caaccgccgc tcgggcgcaa tcgtcgtcgt
      421 gatgcaggta cgcgccccgc cccgcgccgt gcgcggcggg gtccggctgg atccgatcgc
      481 cgccgcggac gaagcgggcg aggtccccct tgggggacga tcgcccgcac ccgacgcagc
      541 ggcgttcggg cgctgctaac tcgcctcctc cgtcgcgggg gcaccctccg gcgtgccgtc
      601 cgcgccggcg gtgggatcgc ccgcgtcggc gtcgggggtc ggcggcggag cctgggccga
      661 gctctccggg tcgggcgagt ccgcctcggc gtcgtcgccc gcggggggct gctccaccgc
      721 gccgccggag cccgggtcgg gctcggccgc ggaggcggag ctctccgggt cgggtgtctc
      781 ggcgacgggc tcgtcctccg ccggaggctg ctcctcggcg ggatccggag cgccctcggc
      841 gaccgggggc ggagcgggct cgccctccgg ggcctcctcg gcggccggct ccggagcggc
      901 ttcgcccgac gggccagcgt cgcccgcggc ctcctcggcg gccggggcca cctcgtcgcc
      961 ctccctgccc tcaagctcct ggtgggccgg gaggccgcag tagcgcgagc cggggagcga
     1021 ggcgttcggg cagcggcggc cgttcgacat gatcgccgcg cagcgccccg ccgtctcctc
     1081 ctcgtcgtag tccatgtcag cctcttcggc agcgaactcg gtctccgacc ggatgtccac
     1141 ccgccagccc gtcaggcggg cggccaggcg ggcgttctgc ccctccttgc cgatcgccag
     1201 cgagagctgg tcgtcgggca cgacgacggt ggcctgcttc gagtcgtcgt ccacgagcac
     1261 ctcgcgcacg cgcgcgggtg agagcgcctt ggcgacgaat cgcgcgggct cctcgttgaa
     1321 cgggatgatg tcgatcttct cgccgcgcag ctcggacacg accatgcgca cgcgcgaccc
     1381 gcgcggaccc acgcaggcac ccaccggatc gacgccgctg gcgtgcgaga cgaccgcgat
     1441 cttcgagcgg tagcccggct cgcgcgcgac gttctgcacc tcgacgagcc cgtcggcgat
     1501 ctccggcacc tcgagttcga acagcttccg gatcagctcg ggcgagcggc gcgagacgat
     1561 gatgctcggg cccttggcct gcgcggagac gtcggtgatg acggccttca cgcgctggcc
     1621 gtggtcgtag cgctcgttgt ccacctgctc ggacttcggg agcagcgcct cgacccgctc
     1681 gcgcagctgc acgagcgtgt agcgcgagtc ggactgctgg acgatgccgg tgatcagctc
     1741 gcccacgcgg tcgcggtact cctcgtacat catgtcgcgc tcggcctcgc ggatccgctg
     1801 gaggatcacc tgcttggccg tctgagcggc gatgcggccg aagtcgtcgg gcgtgacgtc
     1861 gtgctcctcg atctggtcgc ggaactcctc gagcttctcg aggtcgagct cgggctccgg
     1921 gggctcgcgc tgctcgccgg tctcggggtc gacgagcgac tcctcgagct ccacctcgac
     1981 cagcacctgc tcctcgatct cgggcgggat gatcagctcg tagaccttga agtcggcggt
     2041 ctcgcggtcc atctccacgc gcgcgtactg cgccgattcc ggggtcttct tgtacgcgga
     2101 caggagggcg tcctcgagcg cgagcatcag gcgctcggcg gagatcccct tctcccgctc
     2161 aagtacgtgg accgcctcga ctatctcctt ggacatctcc tcatccctcc agcaggttgc
     2221 cgcgcttgac gtcctcgtac gggatcgtga cgaccccttc gtcggccgcg accgtgacct
     2281 cgtcgtcggt cgcgcccacc agctcgccgg tgaagctcct gcgcccgtcg tgctcgccgc
     2341 gggtgcggac gcgcaccttc cggccgacga agcggcgata gtggtccggc ttgctcagcg
     2401 gacgctccgg ccctggcgac gagacctcga gcgagtactc ggtgagcagg tcgcgaaggt
     2461 ggagggtgac gcgctcgcac agcgcaaggc tcacgccttc cggatggtcg acgaagacgc
     2521 gcacgcgctc gcccgcgatg cgctccgcta ggagcacctc cacgtcgggc tcggcggttg
     2581 cgagccgcgt ctcgatctcg gtctgtatgt ctctctccac tctcgtttcc ggcattaaaa
     2641 aaggcgggct gcgaagccca cctccaggtg cggcacgcag gccgcagatg aaagtcctag
     2701 cggccgcgca gtatagccac ggacaccgaa tcacacgggg gcgcgccggg ttcgtaccgg
     2761 catgcgcgta ggactcgcca ccgccatctt cctcgccctc gccgccgccg tcatgtgggc
     2821 ggtgccgtcg ggcgcgcacg actcgctcgc gccccgcgcg gcacaccacc actggctgcc
     2881 caacgagatg tggagccaca agcactgggt gccgtacgac gagtcgcgcc tctacgccct
     2941 tctgggggtc gacacgcgcg agctgttcga gtggctcgcc gacgaccacc acacgatcgc
     3001 ccagctcgcg cgccgccacg gtgtggaccc gcggacgctc gccaagcggc tgatggagcc
     3061 gcggcgcgcg tcgctcagcg gggccgagta ccgcacgctg ctcgaccgcg ccgagcgcac
     3121 gatcactcag gggcacctcg gccagcacgt gctcttccac gtctttcacg gctcgcacct
     3181 gaacgggcac gagaccggcc acatcgagca gctgttcggt gtcgaccgag cgaagtggaa
     3241 ccggctccgg cagcgcggct ggagcccgct gcgcatcgcg acgcaccacg ggcggccggc
     3301 cgacgcggtt cgcgagcacg tgatccagtc gctgcgcgag gagtccgagc gcggcgtgcg
     3361 cgaccacgcc accacgcccg agcaggggga gcggatgctc gcccggcagc tgagggtggt
     3421 cgattgctgg ctgagcagcc cgctcgccaa gaacgacccg tacaacccgt tcggcgacac
     3481 gtggggctgg cacggcccgc acaagcgcgg cgtgcgcaac ggcgtcagca acgcgaagac
     3541 cccgcgcggc tgctgggcga cgctgcccga cgcctgactc tgccgcgatt cgccctcagg
     3601 gcgtgtcgag gacgagggtc acggggccgt cgttgaccag ctcgatctcc atgtgggcgc
     3661 cgaaggtgcc gccggccgcg cccagcgcgg cacgcacgcg ctcgtagagc ggctcggcct
     3721 cggagggcgg cgcggcctcg gcgaagctcg ggcggttgcc cctgcgtgcg tcgccgtaga
     3781 gggtgaagtt gctgacgcag aggatcgcgc cgcccacgtc cccgatcgag cggttcatcc
     3841 gcccctcgtc gtcctcgaac acgcgcaggg cgtggatctt gcgggctagc cagtcggcgt
     3901 cgccgggggt gtcgccgacg cgcacgccga gcaggaccag gaggcccgcg ccgatcgccg
     3961 agacccgctc gccgtcgacg ctcaccgacg cttccgacac ccgctggacg accgctctca
     4021 ctccgtaccc tctcctccat ggccgccgtt gacgtcgtca atgaatacat ggactcgctc
     4081 gagggcgaga cgcgcaagct cgcccacgcc gagtggggcc tcacgctgag cgccgagagc
     4141 gcgggcggct ggccgtgcga catcggggtg cggatcgacg acgggctgct ccgcgtgcag
     4201 gcgtatgcac tcgaggcgcg ggaggacatc gacccggggg tgctcctcca gtggaaccgc
     4261 cagacgcgtc tcgtgcggat ggcctccacg cgcagcggcg acatctgggt gctcgccgac
     4321 ctgcccgtga aggcggtcga cgagcagcag gtcgaccgcc tcctcggact gatcgcggag
     4381 ggcgtgctcg ccgtccgcgg ctatgcgcgt gcgctcaccg agccgccgcc ggcgacaacg
     4441 tccggctggc tcagggcagg accgtgaccg tcttggcgtc ggtgtcgtcg aggcaccact
     4501 cggcgccgct gtccacggtc agcgtcgcgt tgtagctgcc gccgcgcgcg tacacgtggc
     4561 gcacctgctt gccggtcgcg ccgctgccgt cgccgaagtt ccacgtgtag gtctcgatga
     4621 cgctcgggca gctgatctgg aaggtcagcg ggatcgaggc cgagccgtcg aagatcacgg
     4681 gctggccggt gacggccggg ttcggcgcca caatcatgtt cgccttgagc gcgctggcgg
     4741 acgccggcag cacgagcacg gcagcggccg tggccgcgag gacaagcttc ttcacgagtt
     4801 tcccccctgg tcgagtgggg cgcgtatacc ccgcgtcagc gaacgctgaa cgaccgggcg
     4861 ttcttgcgtg aagtgcgccg ctcgaggttg ccgctctggt cggcggccgt gacgcgcagc
     4921 cggtagcgcc cgcgcgggat cttcagcgcc ttcttcagcg accacgagac cttgcgggtc
     4981 ttcttgtcga acttggcctt cgtgcgcagc ttgatcggct ggccgcactt ccggcgcgcg
     5041 ctgagccgcc cgtccgactt cacgaaccgg cagcgcttgc cctcgcgctt ggagatggcg
     5101 accgtcgttg cgacggcctc gcgcccggga caggaacggt ccgcgctgcg gccgctgacg
     5161 gccacccgct tgcggttgag cgtcaggccc ttcttgctga tcgacgagcg cggcgcgctg
     5221 cggtcggcgc acgagtcgat ctggcccgcg atccccgcga gctccggcgg cgtgccgggg
     5281 agcacgtcgc gcgtggagat cgtcgtggcc ggcccgacgt tgccctggtc gtcgaccgct
     5341 cggatcgaca ggtagcgcga cagccgcccg ccgaggccga tgccctcgag cgcgcccggc
     5401 accgccaggt cctgcgcgcg cttgacgacc gggatcgggt cgccctcctc gaacgtcgcg
     5461 ccggtgagtg cgcggtccgg ctgcacgagc tcgtagccgg cgaccttgcc gcacatcagg
     5521 tcgtcgcccg gcgcgcgcag cgagatgcca gcggtgtaga gcttcacgtc gccggccgca
     5581 cccgggttcg acgcgtcgcg cgagtagtcc ccggagttcg cgaggtcgtg gtggaagcgc
     5641 ggccacgatc cgagcgggcg ggccccgctc tggcaggcgg gggcatcggt ccggtaggcg
     5701 agcaccgagc cgccgcgcgt gttggagacc accacgcggc gcgcggccgg atcgctgtcg
     5761 agcgtgccga acgtgccgat cagcgggttc gccacgaccc agtccgaggt gagcttgggc
     5821 catcgcgcga ccggctgccc cgagccgctg taggcctgga ggtcgaggtg agccgaggcc
     5881 gcgacgacct cctccgatgc gcccgcatcc acgttcgcga ccgacgggcc ggtgaggaac
     5941 tggaggtcgt tcatccgcgc cgggaagccc tggcgaaagc gtccgctcgc ggggtcccag
     6001 acggcgatcc cgtcctggcc ggtgttctgg tactcggtga gcaacagatc gagggcccgc
     6061 gtgagtcccg ccacggggct caggagagac accccgccgc cgatgtcgcc gaacgcggga
     6121 tgtcccaggg ccgggaacgc cgggcggtcg gccatgctgc cgttgccgcc gtcggtgtcc
     6181 atcgaggtgt cacggccgtc ctggcgaccc acgcacgacg ttccgtcgcc gttgaacacg
     6241 taggcgacgc cggtgacccc cgcggcgccg accttcgagc ccgcgccgcc gctaggacag
     6301 ttcaccggcc cgatggcggg gttgccggtg acgccctccc cgacaacggg cagcagctcg
     6361 gggagtagca gcgccatctt cgcgggccag ccgggcgcga cccagtcgcc ggtggccggc
     6421 tttccgtcgc gatcgccctc gggcttgatc gcgaagagcc tggtgttcgc tccgacgagc
     6481 tcctgcccca gccgcgacac ggcctgtgcc gtcgccgcgt tgttgccgct gatgttggcc
     6541 ggcccctcgt cgccctggtt cgcggcgtac tcctcgttgg tcccgacgac gatctcgggc
     6601 cgcgcgtcgc ccgcgatgtt cccgaccgcc ggcgtgtcga cgatcgctcc ctgctggagc
     6661 gagccaccgg tcttcttgtc gtcgaacgtg acctgatgcg tgcgcgggtc gatcgacttc
     6721 accttccgca ggtccacgac gaggatcggg tagccgggca cgccggaccc gtcgtcgttc
     6781 agcgcgtaga cgtggcggtc catcgcggcg agcaccacct cgagccggcc gccgtcgtcg
     6841 cggtcgaggt cggccgccac cggcgaagcg aggaagccga gccgggtgcg gttgcgctcc
     6901 cccacgcgcg cgttctcgaa cggagccagc ggcttgccgg agaactcggg attcgcgctc
     6961 ttcctccaga cgagcctgcc ggccgccgac cacgcgtaga ccttcccctc gaaatccgcg
     7021 atcaccacct cgtacgaacc gtcgcggtcg aggtcggcca ccgcgggaga cgagaggacc
     7081 gctccgtgcg aggcgctcgc cggcacctcg ccgctcgcga aggcgcggcc gcccgcgtgc
     7141 agcggcagcg ggtcggtgcg caccggccag cccttcgcct cggacccgtc cgggcgtatc
     7201 gcgtgcaccc agccgtcggc cgtgccgaag acgagctcgt tgcggttgtc gccgtcgagg
     7261 tcggcgaagg cgggagagga ctcgccgtcg ctcgggagcc gcttcgggaa gccgtcgagc
     7321 atgtcctggt cgcggtgcag gaacaggttg cggcggtcct cgccggtggc ggtcaggccc
     7381 gggttctgct ccgcgctctc ggcgatcacg cggaccgtga acccgtaggg ctccgcgttc
     7441 gggcgcccgt tcgaggtctg gccggcaccg tgcccgggct cgggcccgcg gaagtcgccc
     7501 gcgtcggcag ggaagcgctc cttgagggcg ttgacgtcga gcgcggccac cacgccgtcg
     7561 atcggctcgg tgcgcgcctg gccgtcgcag acgggcgagg ggacctgcgc gaagtcaccg
     7621 ggcggcgagt cggtcgtgaa gtggttgttc gggtacgagc ccggcgccac gtagaccttg
     7681 cacgagtact tcgcgccccg cgcccacacc tgggcgtcga cctgggcggt cggcttcgcc
     7741 gggtccacct gcgcgtacca ctccggcgag gtgatctcga cctcgggggc cgtctgcccg
     7801 tcggcggcgc ggcgcaccga ccggaacgtg ttgatgcggc cccagccgta gaactggtcg
     7861 tggccggcgc gcgccgggta gctgcggctg tccggcggcg agacgatcgg ccggttgccg
     7921 ttcacctgct gctgggtgta gaggttcggg tcggtgcagc ccggcgcggg ctcgggcgag
     7981 cacgacggct ccggctgcgg cccgagcacg ctctggttga agttcacgtc gtcgggctgg
     8041 gtctgcccgg cgaacgtgcc cgacgccatc acctgccgga cctcgttggc gctcagcggg
     8101 cacgggcttc cgtccgcgcg cctgcacgtg gggtgcggcc tgagcttccc gttctcgacc
     8161 ccgttcagcg cggcactgag cacgaggccg gccatgccgg cgccgacgcc gaccgcgtcg
     8221 gacgagcagc tcgtgctcgg gatcgcgagg ccgatcttcg aagagaagtt cgtacagccg
     8281 ttgaagctca cgtaggagcg cggctgctgg atcgggacat cggcgcgcgg cggcccgtca
     8341 ggcgtctcct gcgacatgta cttcgtcacc gagttgacca cgagcgtgtg ctcgtggctc
     8401 gacgggtagt tgtggtgctg cgccgcctcg tccgccgccg aggcgatgac gatcacgccg
     8461 tggttgtacg cgtagtcgat cgcgcgctgg gcgagctggg tgttgttgag cgtgccgagg
     8521 gcctcctgga tcacgagcgc gccgttgtcg accgcgtaga gcgtcgcctg ggcgaagtcg
     8581 ttgtcgtcgg cgacgaacga gtcgcccacg cgcatgtgca tcgccatgca gttcgggcag
     8641 gtgccgaccc attcggcgtc gttgttcgcc tccgcactcg acccctccgc ctcgccggtg
     8701 ccgtggtcgt actgcacgtc gtcgaacggg tcgttgtcgt cgtcgaggaa gtcccagccg
     8761 accatgtcgt cggcgaatcc gttgccgtcg gcgtcgctgc cgtcgctgaa cgcgatcagc
     8821 aggtcctgcg ggtcgagcac gtcggtgcgg acgttcttcg gcgtgtcctt gcgcacgcgc
     8881 gagtcgcacg cgtagtcgag gaggttgaag acgttgtcac cgttggcgtc gtgcgcgttg
     8941 gcgtacgagc cgcagttcac gccgccgacg aggtcgggcc ccgagtggtt cggctgcggc
     9001 agctcgcccc ggttcaggaa cgtcttgaag cggaggtcga gcacctggtt cgcgttccag
     9061 cgaaagcccg agtcgagcac ggcgatcttc acgtcgggcc ggccggtcgt caccgtccag
     9121 gcggtctcct tcgtcgcgtc ggcgtccacg acgtgcccgc cgcgcacgcc gaacagctcg
     9181 cgcggctcga ggttcaccgg gaagttgttc tcctccggcg tggccgagaa cttccagacg
     9241 ttctcgtcct tgcgtggcag gtccttcgga atcgccccct tggggacgcg cagcgtcttg
     9301 aagtcgcgct tgtcgccgcc ggactccgag tacgggaact cggcgacggc gacgcgggtc
     9361 atcgcggccg tcatggccac gactgcggtc gatgcgatga acccccggcg caagcagctc
     9421 ccccggcggg agcataggtc aggcagcgcg caggcggtcc cggtacacgc ggtagtcggc
     9481 gcggtccggg cggagctggg cggcgagggc gaggtggcgg cgggcctcgt cgcggcggcc
     9541 gctcttctcg agcgagcggc cgaggcagaa atgcgcgaag tcattcacgg ggtagcgctc
     9601 gaccacggcc gcgaactcct cccccgcgtc ggcgtagcgg cctgagcgga agtacgcccg
     9661 gcctaacgcc tcgcggatcg agctcttgtc gggctcgagg gcgcgggcgc gctcgagcgg
     9721 gatcgcggcg gcggagaagt ccccggcctc gagcagcgcg ctgccgcgct ggaagaggtc
     9781 gtagacgtgc tcgccgcgcg cctccgggga gtcgtgccga tcagccatgc gccactcgtg
     9841 ctgctgtccg cagctcctcg accacgtcca ggatcgaggc ggccacctcc gccttggagg
     9901 cgaacggcac ctcgcgcacg cccgcgggcg tgacgatcgc gacctcgttc tcggcggtgt
     9961 cgaagccgat gtcggggcgg gagatgtcgt tcacgacgat cgcgtcgagg cgcttgcgct
    10021 cgagcttgcc gcgcgcgcgc tcgaccgctc cgtcgccgtg ctctgccgcg aagccgacga
    10081 ccacctgggt gccggtgcgc tggcccgcga gctcgctgag gatgtcctcg gtgggttcga
    10141 gctcgagcgt caggccgacg cggtcgcgct tgccgagctt ggtgtccgcc gggtcagccg
    10201 ggcggaagtc ggctacggca gcggccatga ccaggacgtc ggcgccggcg aactcggcgt
    10261 gtgtggcctc gcggagctgc gcggccgtct ctacgtccac gtagcgcacg ccctcctttc
    10321 gcgggagcgc cacgttggcc gcgaccacgg tcacctcggc gccgcggcga gccgcctcct
    10381 cggcgaccgc gaatcccatg cggcccgacg agcggttgcc cacgaagcgg accgagtcga
    10441 tcggctcgcg ggtgccgccg gccgtgacga gcaggcgcag gccgtcgagc gaccgcggcg
    10501 cgtagggccc cgcgcggagc acctcctcga tcgccgccag gacctccggc ggctcggcca
    10561 gccggccggt gccccactcg ccgcgcgagg cgagcgcgcc ggtggccggc gggacgaccg
    10621 tggcgccgcg ctcgcggagg gtgtccacgt tcgcctgggt ggcggcgtgg ctccacatcc
    10681 tgtcgttcat cgcgggtgcg aggacgagcg gggccgtcga cgcgagtgcg gcgctcgtca
    10741 gcaggttgtc cgccaggccg gtcgcgagct tggcgatggt gttggccgag gccggcacga
    10801 tcgcgtagac gtccgcgcgg cgcacgagct cgaggtgcgc gatcggcgcg tggtcgggcg
    10861 ccgggtcgcc ggggaacgcg ccgcgggcag ggtccgttgc gaactcgtcc accagcacgg
    10921 gcgcgccggt gatgccctcg aacgtggcgc ggcctacgaa gcgctcgctc gacggggtct
    10981 gcaccacgcg gacggagtgc ccggcgcggg tggcgcaacg cacgagctcc acggccttgt
    11041 aggcggagat cccgcccgtc acgccgagga ggatcctggc cattggcggc cgggcgctac
    11101 gtgcggtagc ggtagttgat cttgcccgcg gcgagctctt cgagcgagat cttgaggtag
    11161 ttcttcgaac ccgtatcgac catcggcggc gggtactcgt cgaacgtccc ctcgccgagg
    11221 ttgtggtagt acgagttgat ctggcgggct cgcttggccg cgacgagtac gcacgcgtag
    11281 tgcgagtcga cgcgttcgag caatgtgtcg agtctgggct tgatcaaggt cgccacatgg
    11341 tagcgacgag gcccgtcagc tcgcgcacgg cgggctccag ctcgtcgttg acgatcacat
    11401 gggggaactc gtgttgagcc gccagctcct cctcggcgac ctggagccgg gcgcggatca
    11461 cctcgggcga gtcggtcccc cgcgccagca gacgctcgcg cagcacgtcg gtggacggtg
    11521 gcgcgatgaa cacctggatc gcctcgggaa gcgccgcgcg cacctgccgc gcgccctgga
    11581 cctcgatctc gagcaccacc gggtcgtcct cgacctccga gcgcagcgtg ccgtagcggt
    11641 tgcccgcgta ggtcgcgtgc tcgaggaact ccccggcggc cacccgccgg tcgaagtcct
    11701 cgtgggtcag aaagtggtag tcgaccccgt tctcctcgcc ggagcgcggg gggcgcgtgg
    11761 tggctgagac ggcgaggcgc acgtcaggaa tctccctgca aagcgccttg atcagcgtgc
    11821 ccttgccgac cccggagggg ccggtcacca cgagcactcg tggctttatc ggcgcaggta
    11881 tgagacgagc tcggtccgct ggcgctgcga gagcccgccg atggtcttgc tcggcgagat
    11941 ccggcactgc gtgaggattc gattcacctt cacgcggccg tatttcggca ccgcgaggag
    12001 caggtcgaac accttcgcgg tctggaggta ctcgggcgga tcgaggagca gcccgtggat
    12061 ctgtgcgcgg ccccccttga ggtcgcgctt gagctccgca cggcgtgtgc ggatctcgtt
    12121 ggcgcggcgc agagcgtcca tccgctgcgt gagcgaacgt tccggggccg cgcctacctg
    12181 ggtcttcgtc tgtggcggcg cggcaggcga cacgggcggc gatattacac gcggcgaggg
    12241 gcccgacaag ggcaaaaggg gctaattcgc gtgaatttgg ctggacctcc acctgagctt
    12301 taagccggtt cgggttactt gacggttttt aggcgctttg caggggtttt tttgcggatg
    12361 cttgggcgtt gacgcgccag cgtccgcaaa aggcccgctc agagcgggaa tcggtccgtg
    12421 gcgcaccgac gctcacaggg cccggcggcg ccggggacgg tgccgcgcgg cgcactgggc
    12481 catcttttgg tcgtatagcg accaaaactt ggcccaggtc ggtgaggcga gcgcgagacg
    12541 cggtgcgggc cacccgggcc ggcgccgggt gccctagcgg cgggcggcga cggactcttc
    12601 ggcgcgtcgt ggctccgcgc ccgccgccgc cgccggcgcc gggctgag
//
LOCUS       DASVPL010000150         5076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_330937, whole genome shotgun sequence.
ACCESSION   DASVPL010000150 DASVPL010000000
VERSION     DASVPL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330937"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF517_07780"
     CDS             complement(<1..206)
                     /locus_tag="VF517_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX8052876.1"
                     /translation="MPAMRYREALNKALREEMEADEKVFIMGEDIGVFQGAFKVTQGL
                     LEEFGEKRVRDTPISENTIVGMGVG"
     gene            complement(206..1612)
                     /gene="pdhA"
                     /locus_tag="VF517_07785"
     CDS             complement(206..1612)
                     /gene="pdhA"
                     /locus_tag="VF517_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX8052877.1"
                     /translation="MWRALTWPVRAVLGLVVAIVSVLLARDRRETSQADEPDGVTSGA
                     VAVEEREQRENDGRPQRHHDLPDADTCRAILEKMVLIRRFEERAGEMYAKAKIGGFLH
                     LSIGEEATIVGSIGALRETDYVLSTYREHGQALARGTSPNAVMAELFGRVDGCSKGRG
                     GSMHLFDVERRFLGGYGIVGGNLPLAAGVALASDYTETDDGVLCMFGDGASNQGTFGE
                     TMNLAALWKLPVVFLVVNNQFGMGTALERHSAVTDLSRKSDGFGVPGSKCDGMDVLDT
                     QATIAEAMRKAREDRAPQLVEAVTYRFRGHSMADPEEYRSKEEVEQWRERDPIEAFRK
                     RLADEGVVSEDDYKELDERITKTIDEAVEFADQSPFPDLDTLYEDIYVYGDQVRGWYS
                     VDERSPEPHRGEKEAEAGDVAHELAEAGAAYARVGDAEERSRRQGRGAEAEGGDGDEG
                     SGEGEPDGDERDEEGGAD"
     gene            complement(1642..2130)
                     /locus_tag="VF517_07790"
     CDS             complement(1642..2130)
                     /locus_tag="VF517_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8052878.1"
                     /translation="MLRNDYAGLGNCSVAGTLELIGERWTPLILRDAFLGVRRFDDFQ
                     RDLGIARNVLQARLERLVEAGILRRVLYQERPARYEYRLTRKGIDLWPVIVALMSWGD
                     RHLFPDSPPLLMRHKGCGGLVDDRRTCEDCGALLEPWDVEAERGPGALPTQSLPGAAV
                     RG"
     gene            2219..3619
                     /locus_tag="VF517_07795"
     CDS             2219..3619
                     /locus_tag="VF517_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8052879.1"
                     /translation="MNERRRRALVTAIVVSGVFLASLDLFIVNIAFPDIARDFEDSSL
                     SEMSWILNAYAIVFAALLVPAGRWADRAGRKRGFLYGLALFTLASAVSAAAPSLEVLV
                     GARVLQAGGAALMVPTSLGLLIPEFPAERRAAVVALWAAVGGVAAAAGPPIGGVLVEA
                     SWRWVFLVNIPIGLVALVAGARTLREIRDPSGERPDVLGAAMLAGGIGALTGAIVKGP
                     DWGWGDTPTVALFATAAALVALVVARSRTHPAPVVEPELVRIRSTAFANLAAMLFFAG
                     FGALLLGSVLFLTSVWGESTLSAGLMVAPGPAMAAVFSVPSGLLGERYGARLVGAAGA
                     VVIALSAIWWVSTMGSEPDYAGTFLAPFMLGGVGVGLAIPSITGVATAALPPTRFATG
                     TALVGMSRQIGAALGVAVLVAIVGTPAPGDAVATFGHGWLFVAGAMGATGIAMLLTGP
                     AVVRASAVPTAAGQPA"
     gene            complement(3650..4096)
                     /locus_tag="VF517_07800"
     CDS             complement(3650..4096)
                     /locus_tag="VF517_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052880.1"
                     /translation="MNHALGVVPEWIPVHVAQVDDVDRAAIEAAAARDSLSAVRWPDP
                     FPFDSRTAMLAATFAKQSGRVVAYSLAAMRQAFAAGRDLGVTDNVLIAAAACELHPRA
                     LLKGIEMRSVHTALDAASAGVDAVPALELGDRRLVGAEALADAGIL"
     gene            complement(4182..>5076)
                     /locus_tag="VF517_07805"
     CDS             complement(4182..>5076)
                     /locus_tag="VF517_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8052881.1"
                     /translation="TMWAIDHLPKRIAVIGAGASGTEVASAFGRLGTEVVLLEALDQI
                     LPLEDEEISKIAARELKKQNIEIITGANVENAEAGESEVRLTYGGEEHSFEYVCIAAG
                     RGPDLEDLGLKDAKVKTDERGLVEVDGALRTSVDGVYAIGDMVRGPALAHKASDEGVI
                     AAEDAAGKETHPIDYRFVPAATFCHPQVASFGMTEKEARDEGRDVVIGKVPISAVGAP
                     TVYGDRTGMVKIVGDAEYGELLGGHIVAAKAADLISELVVARDLEGGYSEVARTIHPH
                     PAFPEAVMEAARAADGWLIHG"
BASE COUNT          612 a         1957 c         1717 g          790 t
ORIGIN      
        1 cccacgccca tcccgacgat cgtgttctcc gagatcggcg tgtcgcgcac gcgcttctcg
       61 ccgaactcct cgagcaggcc ctgggtcacc ttgaacgcgc cctggaagac accgatgtcc
      121 tcgcccatga tgaagacctt ctcgtcggcc tccatctcct cgcggagcgc cttgttcaga
      181 gcctcgcggt agcgcatcgc tggcatcagt cggcgccgcc ttcctcgtcg cgctcgtcgc
      241 cgtccggctc gccctcgccg gagccctcgt cgccatcgcc gccctcggcc tcggcaccgc
      301 ggccctgccg ccgcgagcgc tcctccgcgt cacccacgcg cgcgtacgcg gccccggcct
      361 cggccagctc gtgcgcgacg tcgcctgcct cggcctcctt ctcgccgcgg tgcggctccg
      421 gcgagcgctc gtccaccgag taccagccgc ggacctggtc gccgtagacg tagatgtcct
      481 cgtagagcgt gtcgaggtcc gggaacgggc tctggtcggc gaactcgacg gcctcgtcga
      541 tcgtcttcgt gatccgctcg tcgagctcct tgtagtcgtc ctcggacacg acgccctcgt
      601 cggcgaggcg cttgcggaat gcctcgatcg ggtcgcgctc gcgccactgc tcgacctcct
      661 ccttggagcg gtactcctcg ggatccgcca tcgagtggcc gcggaagcgg tacgtcacgg
      721 cctcgacgag ctgtggcgcg cggtcctcgc gcgccttgcg catcgcctcg gcgatcgtgg
      781 cctgggtgtc gagcacgtcc atgccgtcgc acttcgagcc cggcacgccg aacccgtcgg
      841 acttgcgcga gaggtccgtc accgccgagt ggcgctcgag ggccgtgccc atcccgaact
      901 ggttgttgac caccaggaac accaccggca gcttccagag cgcggcgagg ttcatggtct
      961 cgccgaacgt gccctggttg ctcgcgccgt cgccgaacat gcagagcacg ccgtcgtcgg
     1021 tctcggtgta gtcggaggcg agagcgacgc ccgccgccag cggcaggttg ccgccgacga
     1081 tcccgtagcc gccgaggaag cgacgctcga cgtcgaacag gtgcatcgag ccgccgcggc
     1141 ccttcgagca gccgtccacg cggccgaaca gctcggccat aaccgcgttc gggctcgtgc
     1201 cgcgcgcgag cgcctggccg tgctcgcggt acgtggagag gacgtagtcg gtctcgcgca
     1261 gcgcgccgat cgacccgacg atcgtcgcct cctcgccgat cgagaggtgg aggaagccgc
     1321 cgatcttcgc cttcgcgtac atctcgccgg cgcgctcctc gaagcgccgg atcaggacca
     1381 tcttctcgag gatcgcccgg caggtgtcag cgtccgggag gtcgtggtgg cgctgcggcc
     1441 gcccgtcgtt ctcccgctgc tcgcgctcct cgaccgccac cgctccggaa gttaccccat
     1501 cgggttcatc tgcctgcgag gtctcgcggc gatcgcgcgc gagcagcacc gacacgatcg
     1561 cgacgaccag cccgaggaca gcccggaccg gccaggtgag cgcacgccac atggcgcgaa
     1621 tgctcgcgcc ggcgccggac gctagccgcg taccgccgcg cccggcaggc tctgtgtcgg
     1681 cagcgccccc ggaccgcgct cggcctccac gtcccacggc tccagcagcg cgccgcagtc
     1741 ctcgcaggtc cggcggtcgt ccaccaggcc gccgcagccc ttgtgccgca tcagcagcgg
     1801 cgggctgtcg gggaacaggt ggcggtcgcc ccagctcatc agcgccacga tcaccggcca
     1861 gaggtcgatg cccttgcgcg tgagccggta ctcgtagcgc gcggggcgct cctggtagag
     1921 cacgcggcgg aggatccccg cctccaccag ccgctcgagc cgcgcctgga gcacgttgcg
     1981 cgcgatgccg aggtcgcgct ggaagtcgtc gaagcgccgc acgcccagga acgcgtcgcg
     2041 gaggatcagc ggcgtccagc gctcgccgat cagctcgagt gtccccgcca ccgagcagtt
     2101 gcccagtccc gcgtagtcgt tgcgcagcat ctccgtgagt ctagacacat aggttgcttg
     2161 agacaactga ccctggtacg gtccgtcgcg tcatgcaacg gacctgggag agcaagtcat
     2221 gaacgaacgc cggcgccgcg cccttgtcac cgcgatcgtc gtgtcgggcg tgttcctcgc
     2281 gagcctcgac ctgttcatcg tcaacatcgc gttcccggac atcgcccggg acttcgagga
     2341 ctcgtcgctc tccgagatgt cctggatcct caacgcgtac gcgatcgtct tcgccgcgct
     2401 gctcgtgccc gccggccgct gggccgaccg ggcggggcgc aagcgcggct tcctgtacgg
     2461 cctcgccctc ttcacgctcg cctcagccgt ctcggccgcg gcgccgagcc tcgaggtgct
     2521 cgtcggtgcc cgtgtgctcc aggccggcgg cgccgcgctg atggtcccca cctcgctcgg
     2581 cctcctgatc ccggagttcc cggccgagcg ccgggccgcg gtggtggcgc tgtgggcggc
     2641 ggtcggcggc gtggccgcgg ccgcgggccc gccgatcggc ggcgtgctgg tcgaggcgag
     2701 ctggcgctgg gtcttcctcg tcaacatccc gatcgggcta gtcgccctgg tcgccggcgc
     2761 gcgcaccctg cgcgagatcc gcgatccgtc cggcgagcgc cccgacgtcc tcggcgccgc
     2821 catgctggcc ggcggcatcg gcgcgctcac cggcgcgatc gtcaagggcc ccgactgggg
     2881 ctggggcgac acccccacgg tcgcgctgtt cgcgaccgct gccgccctcg tcgcgctcgt
     2941 cgtcgcccgc tcgcgcacgc acccggcgcc ggtcgtcgag cccgagctcg tccgcatccg
     3001 ttccaccgcg ttcgccaacc tcgccgcgat gctgttcttc gccggcttcg gcgccctgct
     3061 gctgggcagc gtgctgttcc tgacgagcgt ctggggcgag tccacgctca gcgccggcct
     3121 gatggtcgcg cccggtcccg cgatggccgc ggtgttctcc gtcccgtccg gcctgctcgg
     3181 cgagcgctac ggcgcacggc tcgtgggcgc cgccggcgcg gtggtgatcg cgctctccgc
     3241 gatctggtgg gtctcgacca tggggagcga gcccgactac gcggggacct tcctcgcccc
     3301 gttcatgctg ggcggcgtgg gcgtgggcct cgcgatcccg tcgatcaccg gcgtggccac
     3361 cgccgccctg cccccgaccc gcttcgccac cggcaccgcg ctggtcggga tgtcgcgcca
     3421 gatcggcgcg gccctcggcg tggccgtgct ggtcgcgatc gtcggcacgc ccgcgcccgg
     3481 ggacgccgtc gccaccttcg gtcacggctg gctgttcgtc gcgggcgcga tgggcgcgac
     3541 cgggatcgcg atgctcctca ccgggccggc cgtggtccgg gcttccgccg tcccgacggc
     3601 ggccgggcaa cccgcctaac gggctcgcga cgagctaaaa ccgcgctcgt tacagaatgc
     3661 ccgcgtccgc cagggcttca gcgccaacga ggcggcgatc ccctagctcg agcgccggca
     3721 ccgcgtccac ccccgcgctc gcggcgtcga gcgccgtgtg gacggagcgc atctcgatcc
     3781 ccttgagcag agcgcgcggg tgcagctcgc acgcggccgc cgcgatcagc acgttgtcgg
     3841 tcacgcccag gtcgcgcccc gcggcgaacg cctggcgcat cgcggcgagc gagtacgcga
     3901 ccacgcgccc ggactgcttg gcgaacgtcg ccgcgagcat cgccgtgcgc gagtcgaacg
     3961 ggaacggatc gggccagcgc acggcgctca gcgaatcgcg ggcggccgcg gcctcgatcg
     4021 cagcccggtc cacgtcatcc acctgcgcca cgtgcacggg aatccactcc ggcaccacgc
     4081 cgagcgcgtg gttcacctgc tcgcccgcga tccagcacca cggcgagccg acgtcgaagt
     4141 agaagcgggg gcgctcgcct cccgccgcgc gcggccggtc gctaaccgtg gatcagccag
     4201 ccgtccgcgg cgcgggcggc ctccatcacg gcctcgggga aggccgggtg cgggtggatc
     4261 gtccgcgcca cctcgctgta gccgccctcg aggtcgcgcg cgaccaccag ctcgctgatg
     4321 aggtcggcgg ccttcgccgc gacgatgtgg ccgccgagca gctctccgta ctcggcgtcg
     4381 ccgacgatct tcaccatgcc ggtccggtcg ccgtagaccg tgggcgcccc cacggcgctg
     4441 atcggcacct tgccgatcac gacgtcgcgg ccctcgtcgc gtgcctcctt ctcggtcatc
     4501 ccgaagctgg cgacctgcgg gtggcagaag gtggcggcgg gcacgaagcg gtagtcgatc
     4561 gggtgcgtct ccttgccggc ggcatcctcc gcggcgatca cgccctcgtc ggatgccttg
     4621 tgcgccagcg ctggtccgcg gaccatgtcg ccgatcgcgt agacgccgtc gaccgacgtc
     4681 cgcagggcgc cgtcgacctc gaccaggccg cgctcgtcgg tcttcacctt cgcgtccttc
     4741 aagccgaggt cctcgaggtc gggaccgcgg cccgcggcga tgcacacgta ctcgaacgag
     4801 tgctcttcgc cgccgtaggt cagccgcacc tcactctcgc cggcctcggc gttctccacg
     4861 ttcgcgccgg tgatgatctc gatgttctgc ttcttcagct cgcgcgcggc gatcttggag
     4921 atctcctcat cctcgagcgg caggatctgg tcgagcgcct cgagcagcac gacctcggtg
     4981 ccgagccgcc caaacgcgga cgcgacctcc gtgcccgagg cgccggcgcc gatcaccgcg
     5041 atccgcttcg gcaggtggtc gatcgcccac atggtc
//
LOCUS       DASVPL010000151        18476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_373045, whole genome shotgun sequence.
ACCESSION   DASVPL010000151 DASVPL010000000
VERSION     DASVPL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18476
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373045"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VF517_07810"
     CDS             <1..616
                     /locus_tag="VF517_07810"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX8052882.1"
                     /translation="RAVRNRRIAAAVGALLILGAVVTVLLVALIDTTVREARAPTTRV
                     VGQLLLNPLQRGGKDQGIAIVAQRGDDRSLIVQARLTPTQKDQAYEVWLYNSDKDAVS
                     LGAQVTDKDGNYQGAGRLPAPLNRYKYIDVSLEKLDRNTAHSGNSVLRGEIANIQAPT
                     EANQPGAQGQAQPGTPQTPGQQPQTPATPGQQPQTPQQQQQPTP"
     gene            complement(644..2032)
                     /locus_tag="VF517_07815"
     CDS             complement(644..2032)
                     /locus_tag="VF517_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052883.1"
                     /translation="MRRRVAAGALAAAALAAFVPPAAARVETPVPAAGPVLAGERVVW
                     ATARADGGVDVRSATGSADARVVQEVRPADAGEPPASRLRPQLAASPARIALVANPAT
                     DSRSWDARVIFTGPPVGPFGRVGGRCGLPGNYPDYPRPVDVDGDAIVYPACDEAGGLE
                     VREFSPAPMTQRILAKGATGARLAGRWVAWLEGGTVWDLDVFAGDLVVYDRLTMREAY
                     RIPRAELGRGVHSLDLQDDGKVAFSFGVGRGHAVGWASPEAPSVHRIPLSERNVYEVR
                     IGAGRIAFLGGRGVNLDVPLAEVGVSDLGGSVRVLASGAQDLIGPESFDFDGTRVAWY
                     TLGCTDATIETRGVDDAQLPYRARSGCALRLNPKPSRRPVLLSGRYVRLYVDCAGFAD
                     RTCRARDLILTSGGRVLARSDRAERIDLTAAGRRAFRGRSKLHVRGTALLTDAAGRRE
                     RRTATFTVRRAS"
     gene            complement(2088..8279)
                     /locus_tag="VF517_07820"
     CDS             complement(2088..8279)
                     /locus_tag="VF517_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052884.1"
                     /translation="MRSHAQDSRVRRQKQLFICLAAVLALAITASAVALGGPSKRARP
                     KAPNVGAAKDTRAAARAADRRDARRAARRAKQLRTPAARRARMRSRSAYGDLHGAATI
                     RLARRNFAEVVARPVWKGLESGNGQRVRRYLNDFTAELEGGSDPGRPAIASSTVPLRT
                     RNASGQAVPVNLTLEPSDDGYAPQAPIVSTRIARRAPGGVSLGSEPIGVRPAGAADVE
                     GSVVSDRVLYPDSAVDTDFIVEPADRGAQVMWQLRSQESPEKHALELDLPNGAKLRQS
                     PQVASAVEVVRGGKLLAFIYPPAAFDADGTQVPVKYAIAGDTVTLDVEHRGADVHYPL
                     LVDPYINVYEDFYDGTWTNSSHAWWASGNGDMNYAAYTFALHLSAKPWVWYSIDQYMG
                     WYWQAPLNSFIYRYDLANIFWYRATWEGSPAGCNSKAQHGLWTGPGYWGWEGGNNAFH
                     QDPTCETDKPDYGLPAICTSWNCDPNAGTNQNYAFAGLSARRDGTGNSAFMGLSRVIL
                     YLRDRYEPRVLSTSQNPTLQDAISNSRWLPGGVMLASSPKGTDWGLGLGRFELVQRAS
                     GRKISVFGSEGNPRPMWCDFSIAAHDTDPWPNPYTDRGGDKGDRNHRCASAQTTPYST
                     PPDSQTASTTFDYSSDHLDEGVNPYGVDAFDIIGTKDSTVSSNWTVKVDRSIPESLAL
                     SGPMTNVWAPASPQPIVSTDANDRLSGVKTTEFDIAAMGDKFERTSTNGWGDAVNGGP
                     WTVNDGPASDFLTESGAGKINFPSGGGYRRATLHQASHRDYDYSGRIKMQSATTGQAV
                     GSFAYFTMRRQANGEAYRVGLVGRYDGLLHVHALNGAGSLLLDVNTGLGFTPGAWYRV
                     RTQVEGSAPTQIRVKVWRDGQPEPGAWQYTAQDSAGPLVAGGTGVQVNGGSTSAQPFY
                     FDDLQALDLGQRQVKESSNPACGNQGCINRHQTSFQWNPTGFAEGAHEYVVRAKDPLG
                     YTRSETRTVKLDRTFPNAPQLSNTLWDKRGTFIKAGTYPLTISASDGNGTNEQSGIEF
                     IRVYVDDPDQAAGTFVELSSSGAAVSGCTASGCPPTNGRPFVLDTAAHNIKEGERTIR
                     VTAIDRAGNPRSTEFKVKFDKFGPAIEPGGPMYTFAQAGEVPNGNHTLTVTATENDPL
                     NPNPPKTARSGSKSIEVFLNGDSIFYDEMTCPAGNCSMTRSTNIDTTEMSSGPHDVEI
                     VSTDQLDNTSTRTFTFRKSCCFGAPTPWGTFSSSTYDLQYADVTGATEGADAITRHKL
                     TGLVQVAPALPGGGFGAPVTWGTWTGATFRRPVDVTGDGRRDLVGLGDGGSIMVGRNL
                     GSAFAPAEANGAWNPALDLHFADLDGDGTTDLVGRDSTGSVTASYFVEGRRLNDFVFG
                     TFSSAYSLDFADVDGDDMADAVGRDSAGNIRVARSEGGYFEPASTWATRDPARYTLAF
                     GDVNGDTMDDLVVRDNSTTAGDVMVAPSDGDTFSSSPAKWATWDKSYEFNLVEANGDG
                     RKDIIGRDAAGVVRASLSNATPPAPENADVDTDEALTDVAVQQPAAPVQTGCQAMGNP
                     TPTTASTPANRPALAFQDDRHLLDRGDLVTQAEGDPFTPSAIPGGAEETAVQRINHIY
                     NRMRQAGANTVRFNVYWGRHEYRRNATNTGWDGPAGQRWYWEDPNNKLDKAINLAACN
                     GFRVLLTLTGTLTGLDCEPGYSGVAARGCEPAEGGTGRATGYDPKPEEYREFVRAAVE
                     HFTVGGVRAQYFSVWNEPNHGQWLDETNGAPPDKRVTHTRYGQLYGAGYAGWFDAGQP
                     AGTRMLIGELSSKPSPGKKTGCNGTAGSCPYTAMEYLQLAAASAKAWMAANVPAALIN
                     GTTVPAHGFAHHPYQHTREPWDANWRKEEVGIAKLYAKTGTRPTVARTLGLMCGTKSG
                     NMCTGAMGSPSGGRTPMYLTEFSYFNRPGGDYKQSGTGWYFEGDRGKKFRSVSTKKPG
                     ALELAYRARAQMMLIYQVIEYPPSDPSQRDQDYGILGPPLTAPPDQDITGNRSYGRRY
                     SDDPTFFPDGRQIKRVAYCKIRAWATDKTHVYFNKTDPIYANSCTAR"
     gene            complement(8432..10042)
                     /locus_tag="VF517_07825"
     CDS             complement(8432..10042)
                     /locus_tag="VF517_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8052885.1"
                     /translation="MTADAPMRWWGWAEPDHPIEVSAAGEALLRSEIGLGDAVTPPVA
                     LEDVRLPDPRPLPELGGVELRQDRETRVRHAAGRSYPDLVRLRSGDGSGAPDAVVYPG
                     SEDEVAAMIAACDAAGVAVVPFGGGTSVVGGVEALHGAHSAAIALDLRRLDSLTVDRS
                     SLTATMGAGLPGRRAEARLQGRGLTLGHFPQSFEFGSVGGYVATRSAGQASTGYGRAD
                     EMVVGLRCVAPAGTLVAKPVPATAAGPSLLQLLVGSEGAFGAITEATLAVRLLPATRR
                     YEGWSFKSFEAGCAAFRAIEQGHVAPDVARLSDEHETRMAMAMSSHGGVAERLGRAYL
                     RARGHEGGCIAIVGWEGEEGQAARRAADTRRVLRAEGGLSLGTSPGQSWLRARYLGPY
                     QRDKLLGRGAFAETLETATTWSNLGSLHRAVGDALREALGARGTPALVACHVSHLYPS
                     GASLYFTFLARAQRGEELEQWAAAKRAAMEAIVASGGTITHHHAVGRDHAEWLREEIG
                     DLGVDALRAVKDRLDPRGIMNPGKLLPG"
     gene            complement(10039..10476)
                     /locus_tag="VF517_07830"
     CDS             complement(10039..10476)
                     /locus_tag="VF517_07830"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8052886.1"
                     /translation="MKVSRATTLPVPPDEVWDLIADPYHLPRWWPRVQRVEEVVGNAW
                     TKVMISSRGRAVRSDYTRVETDAPRRVLWRQEVDESPFERLLSEATIELKLEEAGEGR
                     TRVRLTSEQSLRGKTRFGGMMVRRATRRTLNDALDGLERVFGA"
     gene            complement(10473..10994)
                     /locus_tag="VF517_07835"
     CDS             complement(10473..10994)
                     /locus_tag="VF517_07835"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX8052887.1"
                     /translation="MDLRGHIRDIPDYPKPGIAFKDVTPLFLDPEALDQAIARLAAWA
                     GEREVEYVVAAEARGFILGGAVASRIGAGFVPARKPGKLPRATSSVEYALEYGIDALE
                     VHSDALADGSRVLVHDDLLATGGTAAACCSLVESAGSEVVGCAFLVELAFLGGRARLD
                     GRDVHSLIVYDGE"
     gene            complement(11033..11821)
                     /locus_tag="VF517_07840"
     CDS             complement(11033..11821)
                     /locus_tag="VF517_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8052888.1"
                     /translation="MSTGSVDITGSGRPIDRDEEARRLRERTAQKSLTIGAVCKQLER
                     EFPDISISKIRYLEDQKLLAPRRTQGGYRLYTPADVTRLRTILRLQRDEFLPLRVIRQ
                     ELAAGRADAEVSSSGKGKAPDPRMLRRASVAVTGKGASYRLADVLDDTGAEAKLVKEL
                     VDYGVIGGEQRDGESYYDETEREIIRAVTELTRYGVGGRNLRVFRTSADRESSLLEQI
                     LAPSLRSRNPERRKEAIEALENLAAIVSHLKYLLLVRDLRKIAN"
     gene            complement(11818..12273)
                     /locus_tag="VF517_07845"
     CDS             complement(11818..12273)
                     /locus_tag="VF517_07845"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8052889.1"
                     /translation="MHCPECGFVNTEGSNYCQKCGAFLADVTQEHDNTTAAWQLGETG
                     EIQAVDPEAVADEATLVIRSGGGRSGETFPVEGERMRIGRSPDAEIFLDDVTVSRNHA
                     LLVRRRDGLYIDDLGSLNGTYVNRRRIESHKLADGDELQVGKYKLTYLA"
     gene            complement(12280..12549)
                     /locus_tag="VF517_07850"
     CDS             complement(12280..12549)
                     /locus_tag="VF517_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052890.1"
                     /translation="MDTFPDLGALSDQELKSLIEDLTQQEQEVSYERRILHGKIDILR
                     AELVNRMKKKRESGEAVISGADVDKLTDILAGKGVPAEGPDSDAQ"
     gene            complement(12609..13238)
                     /locus_tag="VF517_07855"
     CDS             complement(12609..13238)
                     /locus_tag="VF517_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8052891.1"
                     /translation="MARARLTKARLLGLDRSGGPPPQTRRGAPLNPWTIPNAIGFVRL
                     GLLPVFLVLAFNSGDGRSVTAAVIYAVVGGTDYLDGIAARVTGQYSRLGALLDPLTDR
                     VLVASGAIVCWYFELLPRWALAVLAIRELVMLFLGQAVLRRGLDIEINMLGRWGVWPT
                     MFSLFLAMLTDTWVADGLLFVGVAMSIAATLQYVRDYSSDPPGAPSSSS"
     gene            complement(13220..14893)
                     /locus_tag="VF517_07860"
     CDS             complement(13220..14893)
                     /locus_tag="VF517_07860"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX8052892.1"
                     /translation="MTRLSSYLLPTVKEAPADAEAASHRLTVRAGLARQLGSGLWTFL
                     PAGWRSHRRVEQIVREELDAIGAQEMLMPVMHPAELWRQTGRYGLTEQFRLQDRKGSD
                     YVLAMTHEEAVTYHVAREVRSYRELPLFLYHFQVKERDEPRPRAGLLRTREFVMKDAY
                     TFDRDAAGLDESYERHAGAYDRIFDRCGLEWYKVESDVGMMGGHGAHEYMAPCAAGEN
                     EVALDGAGYAANMEIASATAQPPGDWAPREGELHTPGATSIEQVAGTLGVPPGALVKA
                     MPYLVGDEPVLVLVRGDHRVNEFKLRNALRADVRQAQDEEIRDRFRAVPGFIGPGLDV
                     RTVADEALRGIDGLVAGANRADYHVGGVTLDGVEWVDVRRVEPGDTSPNGGTIHIEPA
                     IEVGNIFKLGTRYSEPLRATYLDEGGAEHPVWMGSYGIGPARIMMAAVEQFCDEQGIS
                     WPRSIAPFDVHLVALGKPGEEALGVAEGLYKELGSLGLDVLYDDRAASPGEKFADAEL
                     LGCPLRLTVGRKGIEAGEIEAQVRRGQEKRAIPLDGAASAAAELWRGLD"
     gene            14995..17049
                     /locus_tag="VF517_07865"
     CDS             14995..17049
                     /locus_tag="VF517_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8052893.1"
                     /translation="MPRPARRGVLALGLVLGAALALHAVHAVFPFGGDGVDTAITTWL
                     YDGVLLGAALGCALRAALVARCRLPWALLAVGMAAWAAGDLYWSLAVEGTPSEEQVTL
                     GDAGWLSFYPACYVALALLVHARVPRLPRSVWLDGLIGALACGALGAALILPSLTAAA
                     EGAKTATLIVNMAYPVGDLMLLGMVVGVFAVSSWRPDRLWLVLGGGLATSALADGIYL
                     DRLARDSYAAGGLVDTLWLASALLIALAAWQPAPRVRAARRGLRTLVLPVVFGVSAIA
                     LQSYDHFVRINSAALVLATTALIVVMARLFLAFRDNHTMLSASRREALTDALTGLGNR
                     RRLLQDVELALEEGHEPSVLVSYDLDGFKSYNDGFGHPAGDALLARLGQKLSDAVGPK
                     GEAYRMGGDEFCVLVCGGDGRADAAIARCHQALAEQGRGFNVGASHGAVRLPAEADTT
                     AQALQVADHRLYQQKALRPASPRTQARDVLMRVLREREPELHDHLRSVARLATAVARR
                     LGVPPGAVDEIARGAELHDVGKMAVPDAILDKPGSLDAREWSFIHRHTLIGEAVLSAA
                     PALVPVARLVRSSHERFDGAGYPDGLAGESIPLGSRIIFACDAYDAMVSDRPYARAMD
                     PEHALEQIRAGRGTEFDPSVVAALCDAVRAGDHESEEVARLTADDLDAWTPSVPLGGV
                     PR"
     gene            complement(17046..18347)
                     /gene="ispG"
                     /locus_tag="VF517_07870"
                     /gene_synonym="gcpE"
     CDS             complement(17046..18347)
                     /gene="ispG"
                     /locus_tag="VF517_07870"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX8052894.1"
                     /translation="MPSKRQIHVGNVPIGGGAPVAVQSMTKTETADYEATMRQIRAIA
                     DAGGDLVRCAVPREKDVDALRRIVVESPIPVIADIHFNHTLALKSIDAGAHCIRLNPG
                     NIGGPDKVAEVAARAKAAGVPMRIGVNSGSLPKHLHALERENSVEALVQAAVEFVELM
                     ERLEFEDFKVSIKSTSVPNTIASNRLLASRIDYPLHLGITEAGTKWSGSLKSAVGLGT
                     LLADGIGDTVRISLSTFHAEEEVKVAWEILKALKIRQRGPELIACPTCGRLQFDMDTV
                     VAEIEQRLESYEEHIEVAVLGCAVNGIGEASHADFGITGAKNEGLIFAQGKPLRKVPQ
                     ERLVDELFVEIDRSLERGETRFEAKKSAEGAAWLETIENENAGELTPERLAKLEAEQA
                     RAEAAGDGAPANGDGVQLPQVKKLTLDEAASPTEGRRFTRA"
BASE COUNT         2420 a         6687 c         6553 g         2816 t
ORIGIN      
        1 gcgggccgtg cgcaaccgcc ggatcgcggc cgcggtcggg gcgctgctga tcctcggtgc
       61 cgtagtcacc gtcctgctcg tggccctgat cgacacgacc gtgcgcgagg cgcgcgcccc
      121 gaccacgcgc gtggtcggcc agctcctgct gaacccgctc cagcgcggcg gcaaggacca
      181 gggcatcgcg atcgtggccc agcgcggcga cgaccgcagc ctgatcgtgc aggcgcggct
      241 cacgccgacc cagaaggacc aggcatacga ggtctggctc tacaacagcg acaaggacgc
      301 ggtctcgctc ggcgcgcagg tcaccgacaa ggacggcaac taccagggcg ccggccgcct
      361 gcccgccccg ctcaatcgct acaagtacat agacgtctcg ctcgagaagc tcgaccgcaa
      421 caccgcccac agcggcaact cggtgctgcg cggcgagatc gcgaacatcc aggcgccgac
      481 cgaggccaac cagccgggcg cgcagggcca ggcccagcct ggcactccgc agacgccggg
      541 ccagcagccg cagaccccgg cgacgccggg ccagcagccg cagaccccgc agcaacagca
      601 gcagccgacc ccgtagcggc ggcccccggg ggctcggcgg cccctacgag gcgcggcgca
      661 cggtgaaggt ggccgtgcgg cgctcacgcc gtccggcggc atccgtgaga agtgccgtgc
      721 cgcggacgtg cagcttcgag cggccgcgga aggcgcggcg cccggcggcc gtcaggtcga
      781 tccgctcggc acggtcgctg cgcgcgagga cccgcccgcc actcgtgaga atcaggtcgc
      841 gcgcccggca ggtcctgtcc gcgaatcccg cgcagtccac gtacaggcgg acgtagcgcc
      901 cgctcaggag caccggccgc cggctcggct tcgggttcag gcgaagtgcg cagcccgagc
      961 gggcgcggta gggcagctgg gcgtcgtcga caccgcgggt ctcgatcgtc gcgtcagtgc
     1021 agccaagcgt gtaccacgcg acgcgggtgc cgtcgaagtc gaagctctcg ggcccgatga
     1081 ggtcctgggc gccgctggcg agcacccgca cggagccgcc gaggtcggac acgcccacct
     1141 cggcgagcgg cacgtcgagg ttcacgccgc gcccgcccag gaacgcgatc cgcccagctc
     1201 cgatcctgac ctcgtagacg ttgcgctcgg agagcgggat gcgatgcacc gacggcgcct
     1261 cgggggacgc ccagccgacc gcgtgcccac gcccgacgcc gaaggagaag gcgacctttc
     1321 cgtcgtcctg gaggtcgagc gagtgcacgc cccggccgag ttcggcgcgc ggaatccggt
     1381 atgcctctcg catggtgagg cggtcgtaga cgacgaggtc ccccgcgaac acgtcgagat
     1441 cccacacggt gccgccctcg agccacgcga cccagcgtcc cgcgagccgt gcgccggtgg
     1501 cccccttcgc caggatccgc tgggtcatcg gggcggggct gaactcgcgc acctcgaggc
     1561 cgccggcctc gtcgcacgcg gggtacacga tcgcgtcgcc gtccacgtcg acgggccgcg
     1621 ggtagtcggg gtagttgccg ggcaggccgc agcggccgcc cactcgcccg aacggcccaa
     1681 ccggcggtcc ggtgaagatc acccgcgcgt cccagctccg gctgtcggtg gccgggttcg
     1741 caacgagcgc gatgcgggcg ggcgacgccg ccagctgcgg cctcagccgc gacgccggag
     1801 gctctccggc gtccgcggga cgcacctctt ggaccacccg cgcgtccgcg gatccggtcg
     1861 ccgagcgcac gtcgactccc ccatccgcgc gggccgtggc ccataccacg cgctctccgg
     1921 caagcaccgg gccggcggcc ggcacgggcg tttcgacccg cgcggcggcg ggcggcacga
     1981 acgccgcgag tgccgccgcc gcgagtgccc ccgctgctac acgccgtctc acctaccagg
     2041 gaacgcttgg caaacgccta tgttgcgctg cggcgccggc ctcaccctca ccgcgcggtg
     2101 caggagttcg cgtagatcgg gtcggtcttg ttgaaataga cgtgcgtctt gtcggtagcc
     2161 cacgcgcgga tcttgcagta cgcgacccgc ttgatctgcc ggccgtcggg gaagaacgtc
     2221 gggtcgtcgc tgtagcggcg cccatagctg cggttgccgg tgatgtcctg atccggcggc
     2281 gcggtgagcg gtgggccgag aattccatag tcctggtcac gctggctcgg atccgaaggc
     2341 gggtactcga tgacctggta gatgagcatc atctgtgcgc gcgcccggta ggcgagctcg
     2401 agtgctcccg gcttcttcgt gctcacggac ctgaacttct tcccgcggtc gccctcgaag
     2461 taccagccgg tccccgactg cttgtagtcg ccgccggggc ggttgaaata gctgaactcg
     2521 gtcaggtaca tcggcgtcct gccaccgctc ggcgagccca tcgctccggt gcacatgttg
     2581 ccgctcttcg tgccgcacat gaggcccagg gtgcgcgcaa ccgtcgggcg ggtacccgtc
     2641 ttcgcgtaca gcttcgcgat ccccacctcc tccttccgcc agttcgcgtc ccacggctca
     2701 cgcgtgtgct ggtacgggtg gtgtgcgaac ccgtgggcgg ggacggtcgt gccgttgatg
     2761 agcgcggccg gcacgttggc cgccatccac gccttggcgc tcgcggccgc gagctggagg
     2821 tactccatcg cggtgtacgg gcacgacccg gcggtcccgt tgcagccggt cttctttccg
     2881 ggtgacggct tcgatgagag ctccccgatg agcattcgtg ttccggccgg ctgccccgcg
     2941 tcgaaccagc cggcatagcc ggcgccgtag agctgtccgt agcgcgtgtg ggtcacccgc
     3001 ttgtccggcg gggcgccgtt ggtctcgtcg agccactgcc cgtggttcgg ctcgttccag
     3061 accgagaagt actgcgctcg cacgccgccg acggtgaaat gctcgaccgc ggcgcggacg
     3121 aactccctgt actcctccgg cttggggtcg tagccggtgg cgcggccggt gccgccctcg
     3181 gccggctcac agccgcgcgc ggcgacgcct gaatagccgg gttcacagtc gagcccggtc
     3241 agcgtcccgg tgagcgtgag cagtacacgg aacccgttgc aggccgcgag attgatcgcc
     3301 ttatcgagct tgttgttggg gtcctcccag taccagcgct ggcccgcggg cccgtcccag
     3361 ccggtgttcg tggcgttccg cctgtactcg tggcggcccc agtagacgtt gaagcgcacg
     3421 gtgttggcgc cagcctggcg catgcggttg tagatgtggt tgatgcgctg gaccgccgtc
     3481 tcctcggcgc ccccggggat cgcgctcggc gtgaacggat cgccctctgc ctgcgtgacc
     3541 agatcgccgc ggtcgagcag gtgccggtcg tcctggaagg cgagcgcggg ccggttcgcc
     3601 ggcgtggaag ccgtcgtcgg ggtcgggttg cccatcgcct ggcagcccgt ctggacgggc
     3661 gcggcgggct gctggacggc gacgtccgtc agcgcttcgt cggtatcgac gtccgcgttc
     3721 tcgggcgccg ggggtgtggc gttggagagg gacgcgcgca ccacgccggc ggcgtcgcga
     3781 ccgatgatgt ccttgcgccc gtcgccgttg gcttcgacga ggttgaactc gtacgacttg
     3841 tcccaggttg cccacttcgc cgggctcgac gagaacgtgt cgccgtccga gggcgcgacc
     3901 atcacgtccc cggcggtggt gctgttgtcg cgcaccacga ggtcgtccat ggtgtccccg
     3961 ttgacgtctc cgaacgcgag cgtgtaccgc gccggatcgc gcgtggccca cgtcgacgcc
     4021 ggctcgaagt agccgccctc ggaccgggcc acgcggatgt tgcccgccga gtcacgtccg
     4081 accgcgtcgg ccatgtcgtc gccgtcgacg tccgcgaagt cgagcgagta cgcggagctg
     4141 aaggtcccga acacgaagtc gttcagcctc ctgccctcta cgaagtagct cgccgtcacg
     4201 ctgccggtgc tgtcgcggcc gaccaggtca gtggtgccgt cgccgtcgag gtcggcgaag
     4261 tggaggtcga gggccggatt ccatgccccg ttcgcctcgg cgggggcgaa cgccgatccg
     4321 aggttgcgcc cgaccatgat ggaaccgccg tcgccaagtc cgacgagatc ccggcggccg
     4381 tcgccggtca cgtccacggg ccgcctgaag gtcgcgccgg tccaggtgcc ccacgtgacc
     4441 ggcgcgccga agccgccacc cggcagcgcg ggtgccacct gtaccaggcc ggtcagcttg
     4501 tgacgcgtga tcgcgtcagc accctcggtc gctccggtca cgtccgcgta ttgcaggtcg
     4561 tacgtcgacg acgagaacgt gccccacggt gtgggcgccc cgaagcagca gctctttcgg
     4621 aaggtgaacg tcctcgtgga ggtgttgtcg agctgatccg tcgagacgat ctcgacgtcg
     4681 tgcgggcccg acgacatctc cgtcgtgtcg atgttggtcg agcgtgtcat cgagcagttg
     4741 ccggccgggc acgtcatctc gtcgtagaag atgctgtcgc cgttcaggaa cacctcgatc
     4801 gacttggagc ccgagcgcgc ggtcttcggc gggttcgggt tgagcgggtc gttctcggtt
     4861 gccgtgaccg tcagcgtgtg gttgccgttc ggcacctcgc cggcctgggc gaatgtgtac
     4921 atcgggccac cgggttcgat cgcgggcccg aacttgtcga acttgacctt gaactccgtc
     4981 gagcgcggat tgccggcccg gtcgatggcg gtgactcgga tcgtgcgttc accctccttg
     5041 atgttgtggg cggcggtgtc gagcacgaac ggacgcccgt tcgtcggcgg gcatccactc
     5101 gccgtgcagc ccgagacggc ggctccgctc gagctcagct cgacgaacgt cccggcggcc
     5161 tggtcgggat cgtccacgta gaccctgatg aactcgatcc ccgactgctc gttggtcccg
     5221 ttcccgtcgg aggcactgat ggtcaacggg taggtgccgg ccttgatgaa cgtcccgcgc
     5281 ttgtcccaca gcgtgttgct gagctgcggc gcgttcggga acgtccgatc gagcttcacg
     5341 gtgcgcgtct ccgagcgcgt gtagccgagc gggtccttcg ctcggacgac gtactcgtgg
     5401 gcgccctcgg cgaaaccggt ggggttccac tggaacgagg tctgatgccg gttgatgcag
     5461 ccctggttgc cgcaggcggg gttcgagctc tccttcacct gccgctgacc gaggtcgagt
     5521 gcctgcaggt cgtcgaagta gaagggctgc gcgctggtgg agccgccgtt cacctggacg
     5581 ccggttcccc cggccacgag cggcccggcc gagtcctggg cggtgtactg ccaggcgccc
     5641 ggctcgggct gaccgtcgcg ccagaccttc acgcggatct gcgtgggcgc gctgccctcg
     5701 acctgcgtcc tgacccggta ccaggcgccc ggcgtgaagc cgaggccggt gttgacgtcc
     5761 agcaggagac tgccggcgcc gttcagcgca tgtacgtgca gcagaccgtc gtagcggccg
     5821 accagaccga cgcggtaggc ctcgccgttc gcctggcgac gcatcgtgaa gtacgcgaag
     5881 ctgccgaccg cctgaccggt cgtggcgctc tgcatcttga tcctgccgga gtagtcgtag
     5941 tcgcggtgcg acgcctggtg cagcgtcgcc cggcggtagc cgccgcccga cgggaagttg
     6001 atcttccccg ctcccgactc ggtcaggaag tcgctggcgg gtccgtcgtt gaccgtccag
     6061 ggtccgccgt tcacggcgtc gccccagccg ttcgtgctgg tccgctcgaa cttgtcgccc
     6121 atggcggcga tgtcgaactc cgtggtcttg acccccgaga ggcggtcgtt cgcgtcagtg
     6181 ctgacgatcg gttgtgggga cgccggtgcc cacacgttcg tcatcgggcc ggacagcgcc
     6241 aggctctcgg gaatcgagcg atcgaccttc acggtccagt tgctcgagac cgtgctgtcc
     6301 ttcgtgccga tgatgtcgaa ggcgtcgacg ccgtacgggt tcacgccctc gtcgaggtgg
     6361 tcgctggagt agtcgaaggt ggtcgacgcc gtctggctgt cgggcggcgt gctgtatggc
     6421 gtcgtctgcg ccgacgcgca ccggtggttg cggtcgccct tgtcgccgcc gcgatcggtg
     6481 tacgggttcg gccaggggtc ggtgtcgtga gcggcgatcg agaagtcgca ccacatcggg
     6541 cgcgggttgc cctcgctgcc gaacacgctg atcttgcggc cgctggcgcg ctgcacgagc
     6601 tcgaagcgcc cgaggccgag gccccagtcg gtccccttcg gcgagctggc gagcatgacg
     6661 ccgccgggca gccagcggct gttcgagatc gcgtcctgga gcgtcgggtt ctggctcgtc
     6721 gagagaacgc gcggctcgta gcggtcccgc aggtacagga tcacgcggga aagacccatg
     6781 aaggcactgt tgccggtgcc gtcgcgtcgc gcggagagac ccgcgaacgc gtagttctgg
     6841 ttggtgcctg cgttcgggtc gcagttccac gaggtgcaga tggcgggcag cccgtagtcg
     6901 ggcttgtcgg tctcgcaggt ggggtcctgg tggaacgcgt tgttgccgcc ctcccagccc
     6961 cagtagccgg gcccggtcca cagtccgtgt tgcgccttgc tgttgcaccc tgccggcgag
     7021 ccctcccacg tcgcgcggta ccagaagatg ttcgcgaggt cgtagcggta gatgaacgaa
     7081 ttcagcggcg cctgccagta ccaacccatg tactggtcga tcgagtacca gacccacggc
     7141 ttcgccgaga gatgcagggc gaacgtgtag gccgcgtagt tcatgtcgcc gttgccggag
     7201 gcccaccacg cgtgcgagct gttcgtccag gtcccgtcgt agaagtcctc gtagacgttg
     7261 atgtaggggt ccacgagcag cgggtagtgg acgtccgcgc cccgatgctc gacgtcgagc
     7321 gtgaccgtgt cgccggcgat ggcgtacttg acgggcacct gggtgccgtc ggcgtcgaac
     7381 gcggccggcg gatagatgaa ggcgagcagc ttcccgccgc gcacgacctc gacggcgctc
     7441 gcgacctgcg gcgactggcg cagtttcgcg ccgttcggga gatcgagctc gagcgcgtgc
     7501 ttctccgggc tctcctggga gcgcagctgc cacatcacct gggcgccgcg atcggcgggc
     7561 tcgacgatga agtccgtgtc gacggcgctg tcggggtaga gaacgcggtc cgagacgacc
     7621 gagccctcga cgtcggcggc gcccgccgga cgcacgccta tcggctcgct gccgaggctc
     7681 acgccgcccg gggcgcgcct ggcgatgcgc gtgctgacga tcggcgcctg cggggcgtac
     7741 ccgtcgtcgc tcggctcgag cgtgaggttg acgggaacgg cctgacccga cgcgttgcgc
     7801 gtgcgcagcg gcaccgtcga gctggcgatc gccggcctgc ccgggtcgct cccaccctcg
     7861 agctcggccg tgaagtcgtt gagatagcga cgcacgcgct ggccgttgcc ggactcgagc
     7921 cccttccaga ccggtcgtgc gacgacctcg gcgaagttgc gcctggcgag ccggatcgtt
     7981 gccgccccgt gcagatcgcc gtacgcgctg cgcgaccgca tccgcgccct acgcgcggcc
     8041 ggggtgcgca gctgcttcgc ccgccgtgcc gctcgtcgtg cgtcacgcct gtcggcggcc
     8101 cgcgccgccg cacgggtgtc cttcgccgcg ccgacgttgg gcgccttggg cctcgcccgc
     8161 ttgctcgggc cgccgagcgc gacagcgctg gcggtgatgg ccagcgcgag gaccgccgcc
     8221 aggcagatga agagctgctt ctgtcggcgc acacgcgagt cctgcgcgtg tgagcgcatg
     8281 cgaagtccac gcatgtcttc ccccgttcag ttgcgagtcc gctctacgcg gttgccaccc
     8341 ccgcggcttc tcgaccctaa ccgcattgcg caaaaagcgc aatagcgaaa aaccgatctt
     8401 tacgtgcgga cgccggtgtg agccagcgtt cctagcccgg cagcagcttc cccgggttca
     8461 tgatcccgcg cgggtcgagc cggtccttga ccgcccggag agcgtcgacg cccaggtcgc
     8521 cgatctcctc ccgcagccac tcggcatggt cgcggccgac cgcgtggtgg tgggtgatcg
     8581 tgccgccgct ggccacgatc gcctccatcg cggcgcgctt ggcggccgcc cactgctcga
     8641 gctcctcgcc gcgctgcgcg cgcgccagga acgtgaagta gagcgacgcg cccgagggat
     8701 acaggtggga gacgtggcag gccacgagcg ccggcgtgcc gcgcgcgccg agcgcctcgc
     8761 gcagcgcgtc gcccacggcg cggtggagcg agccgaggtt gctccatgtc gtggcggtct
     8821 cgagcgtctc cgcgaacgcc ccgcggccga gcagcttgtc gcgctggtag gggccgaggt
     8881 agcgcgcgcg cagccacgac tggcccgggc tcgtgccgag cgacagcccg ccctccgcgc
     8941 gcagcacccg tcgcgtgtcg gcggcccgcc gcgcggcctg cccctcctcg ccctcccagc
     9001 cgacgatggc gatgcagccg ccctcgtgcc cgcgggcgcg taggtatgcc ctgccgagcc
     9061 gctccgcgac accgccgtgc gaggacatcg ccatcgccat gcgcgtctcg tgctcgtccg
     9121 agagccgcgc cacgtcgggc gcgacgtggc cctgctcgat cgcgcgaaac gccgcacagc
     9181 cggcctcgaa gctcttgaac gaccacccct cgtagcggcg cgtggcgggc aggagccgca
     9241 ccgcgagcgt cgcctcggtg atggctccga acgcgccctc cgaccccacg aggagctgga
     9301 gcagcgaggg ccccgcggcc gtggccggca ccggcttcgc cacgagcgtg cccgcgggcg
     9361 cgacgcagcg cagtccgacc accatctcgt ccgcgcggcc gtagccggtc gaggcctgcc
     9421 cggccgagcg cgtggccacg tagccgccga cgctgccgaa ctcgaacgac tgcgggaagt
     9481 ggccgagcgt gagcccgcgg ccctggagcc gcgcctccgc gcggcggccg ggcagccccg
     9541 cccccatcgt cgcggtgagc gaggagcggt ccacggtcag cgagtcgagc cgacggaggt
     9601 cgagggcgat cgcggcggag tgcgcgccgt gcagggcctc gacgccgccc accacgctcg
     9661 tcccgccgcc gaacggcacg accgcgacgc ccgcggcgtc acaggcggcg atcatcgccg
     9721 ccacctcgtc ctcgctgccg ggatacacca ctgcatcggg cgcccccgac ccgtcgcccg
     9781 agcgcagccg cacgaggtcc gggtagctgc gcccggcggc atggcggacc cgcgtctcgc
     9841 ggtcctggcg aagctccacc cctccgagct cggggagcgg cctcgggtcc ggcaggcgca
     9901 cgtcctcgag cgcgaccggc ggcgtgaccg cgtcgccgag cccgatctcg ctccgcagca
     9961 gcgcctcgcc cgccgccgag acctcgatcg ggtggtcggg ctcggcccag ccccaccagc
    10021 gcatcggcgc gtcggcggtc atgccccgaa cactcgctcg agcccgtcga gcgcgtcgtt
    10081 cagcgtgcgc cgcgtcgcgc gccgcaccat catcccgccg aaccgggtct tgccccgcaa
    10141 cgattgctcg ctcgtcagcc gcacgcgcgt gcgcccctcc cccgcctcct cgagcttcag
    10201 ctcgatcgtg gcctccgaca gcagtcgctc gaacggcgac tcgtcgacct cctggcgcca
    10261 cagtacccgt cgcggcgcgt cggtctcaac gcgcgtgtag tccgaccgca cggcccggcc
    10321 gcgtgaggag atcatcacct tcgtccaggc gttgcccacg acctcctcga cccgctgcac
    10381 gcgcggccac cagcgcggga ggtggtacgg gtcggcgatc aggtcccaga cctcgtcggg
    10441 gggcacgggg agcgtggtcg cgcgcgagac cttcactcgc cgtcgtagac gatcaggctg
    10501 tgcacgtcgc ggccgtcgag ccgcgcgcgg ccgccgagga acgccagctc caccaggaac
    10561 gcgcagccga ccacctcgga cccggccgac tcgaccagcg agcagcacgc cgcggccgtg
    10621 ccgccggtgg ccagcaggtc gtcgtgcacc agcacgcgcg agccgtccgc cagagcgtcg
    10681 ctgtgcacct cgagggcgtc gatgccgtac tcgagcgcgt actccaccga cgaggtcgcg
    10741 cgcgggagct tgccgggctt gcgcgcgggc acgaaccccg cgccgatgcg cgacgccacc
    10801 gcgccgccca ggatgaaccc gcgcgcctcg gcggccacca cgtactccac ctcgcgctcg
    10861 ccggcccagg ccgcgagccg cgcaatcgcc tggtcgagcg cctcggggtc gaggaacagc
    10921 ggggtgacgt ccttgaacgc gatccccggc ttcgggtagt cggggatgtc gcggatgtgg
    10981 ccccggaggt ccacgcgcgc tcggctagcg cctcggcccg cggccggcca cgctagttcg
    11041 cgatcttgcg caggtcgcgc acgagcagca ggtacttcag gtgcgagacg atcgccgcca
    11101 ggttctcgag cgcctcgatc gcctccttgc gccgctccgg gttgcgcgag cgcagcgacg
    11161 gggcgaggat ctgctcgagc agcgacgact cgcggtcggc ggaggtgcgg aacacgcgca
    11221 ggttgcgccc gccgacgccg tagcgcgtca gctcggtcac ggcgcggatg atctcgcgct
    11281 cggtctcgtc gtagtaggac tcgccgtcgc gctgctcgcc accgatcacg ccgtagtcca
    11341 cgagctcctt cacgagcttg gcctcggcgc ccgtgtcgtc gagcacgtcc gcgagccggt
    11401 acgaggcgcc cttgccggtc acggccaccg aggcgcgccg cagcatgcgc gggtcgggtg
    11461 ccttcccctt gccggaggag gagacctccg cgtccgcccg gccggcggcc agctcctgcc
    11521 tgatcacgcg cagcgggagg aactcgtcgc gctggaggcg caggatcgtg cgcagccgcg
    11581 tcacgtccgc gggcgtgtag agccggtagc cgccctgcgt gcggcgcggc gccagcagct
    11641 tctggtcctc gagatagcgg atcttcgaga tcgatatgtc cgggaactcg cgctcgagtt
    11701 gcttgcagac ggccccgatc gtgagcgact tctgcgccgt gcgctcgcgc aggcggcgcg
    11761 cctcctcgtc gcggtcgatc ggccgcccgg atcctgtgat gtccacggag cccgtgctca
    11821 tgcgaggtag gtgagcttgt acttgccgac ctggagctcg tcgccgtccg cgagcttgtg
    11881 cgactcgatc cggcggcggt tcacgtaggt gccgttcaac gacccgaggt cgtcgatgta
    11941 gaggccgtcg cggcggcgga cgagcagagc atggttgcgg gagactgtca cgtcgtcgag
    12001 gaagatctcc gcgtcggggc tgcggccgat ccgcatccgc tcgccctcga cggggaaggt
    12061 ctcgccggag cgcccgcctc cggagcggat gacgagcgtc gcctcgtcgg cgacggcctc
    12121 ggggtcgacg gcctggatct caccggtctc gcccagctgc caggcggcgg tggtgttgtc
    12181 gtgctcctgg gtcacgtcgg ccaggaaagc accgcacttc tggcagtagt tcgatccctc
    12241 ggtgttgacg aacccgcact cggggcagtg catggtgcgc tactgcgcgt cggagtccgg
    12301 gccctcggcg gggacgccct tgcccgcgag gatgtcggtg agcttgtcca cgtcggctcc
    12361 gctgattacc gcctcgccgg actcgcgctt cttcttcatc cggttgacga gctcggcgcg
    12421 caggatgtcg atcttgccgt gcaggatgcg ccgctcgtac gaaacctcct gctcctgctg
    12481 cgtgaggtcc tcgataaggc tcttgagctc ctggtccgac agcgcgccga gatccgggaa
    12541 ggtgtccatg ggtcgtccta ggtcgtggga cgggaggcgg cagtataccc ctgcggcgcc
    12601 gccaactgtc aactcgagct tgagggtgcg cccggcgggt cggagctgta gtcgcgcacg
    12661 tactggagcg tcgcggcgat gctcatggcc acgccgacga acagcagccc gtccgccacc
    12721 cacgtgtcgg tgagcatcgc gaggaacagc gagaacatcg tcggccagac cccccagcgg
    12781 ccgagcatgt tgatctcgat gtcgagcccg cgccggagca cggcctggcc gaggaacagc
    12841 atcacgagct cgcggatcgc aaggaccgcc agggcccagc gcgggagcag ctcgaagtac
    12901 cagcagacga tcgcgccgga ggcgactagc acgcggtcgg tgagcgggtc gagcagcgcg
    12961 ccgaggcggc tgtactggcc ggtcacgcgc gcggcgatgc cgtcgaggta gtcggtgccg
    13021 cccaccacgg cgtagatgac cgccgccgtg accgagcgcc catcgccgga gttgaacgcg
    13081 agcacgagga agaccggcag cagccccagc cggacgaagc cgatcgcgtt cgggatcgtc
    13141 cacgggttga gcggtgcgcc gcgccgggtc tgcggcggcg ggccgccgga gcggtcgagc
    13201 ccgagcaggc gggccttggt cagtcgagcc cgcgccacag ctccgccgcc gcggatgccg
    13261 ccccgtcgag cggtatcgcc cgcttctcct gcccgcgcct cacctgggcc tcgatctcgc
    13321 cggcctcgat gcccttgcgc ccgacggtca gccgcagcgg gcagccgagc agctcggcgt
    13381 cggcgaactt ctcccccggg ctggccgcgc ggtcgtcgta gagcacgtcg agccccagcg
    13441 agccgagttc cttgtagagg ccctcggcca cgccgagcgc ctcctctccc ggcttgccga
    13501 gcgcgaccag atgtacgtcg aacggcgcta tcgagcgcgg ccacgagatg ccctgctcgt
    13561 cgcagaactg ctccaccgcg gccatcatga tgcgggccgg gccgatgccg taggagccca
    13621 tccagaccgg gtgctcggcg ccgccctcgt cgaggtaggt cgcgcgcagc ggctcggagt
    13681 agcgcgtgcc gagcttgaag atgttgccga cctcgatcgc cggctcgatg tgaatcgtgc
    13741 cgccgttcgg cgaggtgtcg cccggctcga cgcggcggac gtccacccac tcgacgccgt
    13801 ccagcgtgac cccgccgacg tggtagtccg cccggttcgc gcccgccacc aggccgtcga
    13861 tgccgcgcag cgcctcgtcg gcgacggtcc ggacgtcgag gccgggcccg atgaagccgg
    13921 gaaccgcgcg gaagcggtcg cggatctcct cgtcctgggc ctggcgcaca tcggcgcgca
    13981 gggcgttgcg gagcttgaac tcgttgacgc ggtgatcgcc ccggacgagc acgaggacgg
    14041 gctcgtctcc caccaggtag ggcatcgcct tgaccagcgc accgggcggc acaccgagcg
    14101 tgccggccac ctgctcgatg ctcgtcgccc ccggcgtgtg cagctcgccc tcccgcggcg
    14161 cccagtctcc cgggggctgc gcggtggcac tcgcgatctc catgttcgcc gcgtagccgg
    14221 cgccgtcgag cgcgacctcg ttctcgcccg cggcgcaggg agccatgtac tcgtgggccc
    14281 cgtggccgcc catcatcccc acgtccgact cgaccttgta ccactcgagt ccgcagcggt
    14341 cgaagatccg gtcgtaggcg cccgcgtggc gctcgtagga ctcgtcgagg cccgccgcgt
    14401 cgcggtcgaa cgtgtaggcg tccttcatca cgaactcgcg cgtgcgcagc agccccgcgc
    14461 gcgggcgcgg ctcgtcgcgc tccttgacct ggaagtgata gaggaacagc ggcagctcgc
    14521 ggtaggagcg gacctcgcgg gccacgtggt aggtcacggc ctcctcgtgg gtcatggcga
    14581 gcacgtagtc gctgcccttg cggtcctgga ggcggaactg ctcggtcagg ccgtagcgcc
    14641 cggtctggcg ccacagctcg gccgggtgca tgaccggcat cagcatctcc tgcgcgccga
    14701 tcgcgtcgag ctcctcgcgc acgatctgct ccacgcggcg gtgcgagcgc cagcccgcgg
    14761 gcaggaaggt ccagagcccg gagccgagct ggcgcgccag cccggcgcgc accgtcagcc
    14821 ggtgcgaggc ggcctcggcg tcggcggggg cctccttcac ggtggggagc aggtacgagc
    14881 tgaggcgcgt caccgccgtc agcctattcg gtggtcaccg acttgctgta tcgagcgccg
    14941 gcgcgcccgt gccgatgtag acccatgcgc tccagcgcac cagagctcga tgccgtgccc
    15001 cggccggccc gtcgcggcgt gctcgcgctc ggcctcgtgc tgggcgcggc cctcgctcta
    15061 catgccgtcc acgcggtctt tccgttcggc ggcgacgggg tggatacggc gatcacgacc
    15121 tggctctacg acggcgtgct gctcggcgcg gcgctgggct gcgcgctgcg cgccgccctg
    15181 gtcgcgcgct gccggctgcc ctgggcgctg ctcgccgtcg ggatggcggc gtgggccgcc
    15241 ggcgacctct actggagcct cgcggtcgag ggcaccccga gcgaggagca ggtcacgctc
    15301 ggcgacgccg gctggctcag cttctacccc gcctgctacg tcgcgctggc gctgctcgtc
    15361 cacgcgcgcg tgccgcgact gccccggagc gtgtggctcg acggcctgat cggcgccctc
    15421 gcctgcggcg cgctcggcgc cgcgctgatc ctcccgagcc tcaccgccgc ggcggaggga
    15481 gccaagacgg cgaccctgat cgtgaacatg gcctacccgg tcggcgacct gatgctgctc
    15541 gggatggtcg tgggcgtgtt cgccgtctcg agctggcggc ccgaccggct ctggctcgtg
    15601 ctcggcggcg gcctcgccac cagcgcgctc gccgacggca tctacctcga ccgcctggcc
    15661 cgcgacagct acgccgcggg cgggctcgtg gacaccctct ggctcgcctc cgctctgctg
    15721 atcgccctcg ccgcctggca gcccgccccg cgggtccgcg cggcgcgacg cgggctgcgg
    15781 acgctcgtgc tgccggtcgt gttcggggtg agcgcgatcg cgctccagtc ctacgaccac
    15841 ttcgtgcgca tcaacagcgc ggctctggtg ctggccacga cggcgctgat cgtcgtgatg
    15901 gcgcgcctct tcctcgcctt ccgcgacaac cacacgatgc tctcggccag ccgccgcgag
    15961 gcgctcaccg acgcgctcac cggactcggc aaccggcgcc ggctgctcca ggacgtcgag
    16021 ctggcgctcg aggagggtca cgagccgagc gtgctcgtct cctacgacct cgacgggttc
    16081 aagtcctaca acgacggctt cgggcacccg gccggcgacg ccctgctggc gcgcctcggc
    16141 cagaagctct cggacgcggt cggccccaag ggcgaggcgt accggatggg cggcgacgag
    16201 ttctgcgtgc tcgtctgcgg cggcgacggt cgcgccgacg ccgcgatcgc ccgctgccac
    16261 caggctctgg ccgagcaggg gcgcggcttc aacgtcggcg cctcgcacgg cgcggtgcgg
    16321 cttcccgcgg aggccgacac gaccgcgcag gcgctacagg tcgccgatca ccggctctac
    16381 cagcagaagg ccctccggcc cgcgtcgccg cgcacgcagg cgcgcgacgt gctgatgcga
    16441 gtcctgcgcg agcgcgagcc cgagctgcac gaccacctgc gctcggtggc acggctggcc
    16501 accgccgtcg cccggcgcct gggggtgccg cccggcgccg tcgacgagat cgcccgcggc
    16561 gccgagctcc acgacgtcgg caagatggcc gtaccggacg cgatcctcga caagcccggg
    16621 tcgctcgacg cgcgcgagtg gagcttcatc caccgccaca cgctgatcgg cgaggcggtg
    16681 ctgtccgccg cgccggcgct cgtgccagtc gcgcggctcg tccgctcctc gcacgagcgc
    16741 ttcgacggcg ccggctaccc cgacgggctg gcgggcgaga gcatcccgct cggctcgcgg
    16801 atcatcttcg cctgcgacgc ctacgacgcg atggtcagcg accggcccta cgcgcgggcg
    16861 atggacccgg agcacgcgct cgagcagatc cgcgccgggc gcggaaccga gttcgacccg
    16921 tcggtggtcg cggcgctctg cgacgcggtc cgagccggcg accacgagag cgaggaggtg
    16981 gcgcgcctca cggccgacga cctcgacgcg tggacgccga gcgtgccgct cggcggcgtc
    17041 ccccgctagg cccgggtgaa gcggcggccc tcggtgggcg acgccgcctc gtccaacgtc
    17101 aacttcttca cctggggaag ctggaccccg tcgccgttgg cgggagcgcc gtcgccggcc
    17161 gcctccgccc gcgcctgctc ggcctcgagc ttcgcgagcc gctccggggt gagctcaccc
    17221 gcgttctcgt tctcgatcgt ctcgagccag gcggcgccct cggcggactt cttggcctcg
    17281 aaccgggtct cgccgcgctc gagcgagcgg tcgatctcca cgaacagctc gtccacgagc
    17341 cgttcctgcg gcaccttgcg cagcggcttg ccctgggcga agatcaggcc ctcgttcttg
    17401 gcgcccgtga tgccgaagtc ggcgtgcgag gcctcgccga tcccgttcac ggcgcagccg
    17461 agcacggcca cttcgatgtg ctcctcgtag gactcgaggc gctgttcgat ctcggcgacg
    17521 accgtgtcca tgtcgaactg gagccggccg caggtcgggc aggcgatcag ctcggggccg
    17581 cgctggcgga tcttcagcgc cttgaggatc tcccaggcga ccttgacctc ctcctcggcg
    17641 tggaaggtcg agagcgagat gcggaccgtg tcgccgatgc cgtcggccag cagcgtgccg
    17701 agccccaccg ccgacttgag cgagcccgac cacttcgtgc cggcctcggt gatgccgagg
    17761 tggagcgggt agtcgatccg gctcgcgagc aggcggttcg aggcgatcgt gttcggcacg
    17821 ctcgtcgact tgatcgagac cttgaagtcc tcgaactcga gtcgctccat cagctcgacg
    17881 aactcgacgg ccgcctgcac gagcgcctcg accgagttct ctcgctctag cgcgtgcagg
    17941 tgcttgggca gcgagccgga gttgacgccg atccgcatcg gcaccccggc ggccttggcc
    18001 ctggcagcca cctcggcgac cttgtcgggg ccgccgatgt tgccggggtt cagccgtatg
    18061 cagtgggcgc cggcgtcgat cgacttgagc gcgagcgtgt ggttgaagtg gatgtcggcg
    18121 atcaccggga tcggggactc gacgacgatc cgccgcaacg cgtcgacgtc cttctcgcgc
    18181 ggcaccgcgc agcggacgag atcgccgccg gcgtcggcga tcgcgcggat ctggcgcatg
    18241 gtcgcctcgt agtcggcggt ctcggtcttc gtcatcgact gcacggcgac cggcgcgccc
    18301 ccgccgatgg gcacgttccc gacgtggatc tgccgcttgg aaggcatcac ctagagggta
    18361 gtgagccggc gagtttccat cggcattcct gacttaaggg tccatacgac ccataagtca
    18421 ggaatgtcaa cgatttgcgg gctcgatccg ccaggtcacg gggcccggag ccggcc
//
LOCUS       DASVPL010000152         3560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_292599, whole genome shotgun sequence.
ACCESSION   DASVPL010000152 DASVPL010000000
VERSION     DASVPL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292599"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1628)
                     /gene="asnB"
                     /locus_tag="VF517_07875"
     CDS             complement(132..1628)
                     /gene="asnB"
                     /locus_tag="VF517_07875"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF006949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HEX8052895.1"
                     /translation="MCGIVAAHGVSDRAALEAMLARLAHRGPDDSGSVAVNGSWLGHR
                     RLSIVDVGGGAQPLHDPDGELFLVGNGEVYNHEAVRDGLADRPFLTSSDNEVALHLLD
                     ERGPESLAELNGMFAFVAAGRDGRFLAARDPVGIKPLYWARRGDEVLFASEMHVFDPS
                     WQPAVEPFPPGCAWTPETGLVRFARALPEDPEPVPAHRVVSGTRDVLIESVHRQLMGD
                     VPVGVFLSGGLDSSLVAAIAARILAARGERLKTFAVGTPGSPDLAAARRVAEHLGCEH
                     HETTYSAADALEALPGVVRSIESFDPGLVRSAVPNYVLARFTAQHVKVVLTGEGADEL
                     FAGYAYMRDFTDAGALHAELERTICSLHNLNLQRCDRVTMAHGLEARVPFLDRAVIDW
                     AMALPPEAKLAPPGTPEKTLLREAFEGWLPHDLLWRDKAEFGDGSGARDVLSEAVAAD
                     IIDAEFDAEFHAVDPPLRTKEELAYYRIFREHLPHVRAEVAISRFARA"
     gene            complement(1638..2513)
                     /locus_tag="VF517_07880"
     CDS             complement(1638..2513)
                     /locus_tag="VF517_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX8052896.1"
                     /translation="MEREPTIAAVAAPFGRDIDAAFARVERTLVAARRRGARLVVFPE
                     CALGGYLREPLPGEIGPDLPPALDPDGPEIARLIRLAGETVVCIGYTEAAPGGPYSSA
                     VCVSGDGVLGRHRKVHLPPAERFAYSAGDGFAAFDTPAGRVGMLLCYDKLFPEAARAL
                     ALDGAETIACMASWPVDRLNPAARSSADRQARHFDLMAAARAVENQVVWVSSNQSGPW
                     GPLRFLGRAQVVDPDGVVRARTGARAGVAVAHLDARTEIGALRAHIDHLGDRRAHAYG
                     HVAGAAAALPAAARG"
     gene            complement(2597..3022)
                     /locus_tag="VF517_07885"
     CDS             complement(2597..3022)
                     /locus_tag="VF517_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052897.1"
                     /translation="MSYFVTIVNSTDAPMTTKDGQTIAAGDTWRSGVNGNTYVHSEEF
                     GSVSFRDIADQHIGGDTDETWGVLISYNGQHMVGRYEGGGELHVTFNNRLQAELSGMN
                     LRRVRLDSLVSDHDKLLAGTVRDAPDAPDAPTESRPETS"
     gene            3131..>3560
                     /locus_tag="VF517_07890"
     CDS             3131..>3560
                     /locus_tag="VF517_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052898.1"
                     /translation="MPLLLLVIAAALAAAGCGGDDGGGPAAPAPTTSTQAEPAPAPTT
                     GEAEPRESDEPKPRGERRERTPRSLAGCIKDAPGVEDALVKGRDSEDATFFADLVGGR
                     VDVLGVTLSGESAEVGVFLFATPAAAAKAAPQAGGGGTVAR"
BASE COUNT          460 a         1267 c         1370 g          463 t
ORIGIN      
        1 ggacctcgag ctccagggcc tcgacgccga cgcgcgcacc cctgggctgc ccgcgcacct
       61 cgacggctgc ccggcctgcc gcgaggagca cgagggcctg cgcgagctgg cccgcggcgg
      121 ctgatcccgc cctaggcccg ggcgaaccgg ctgatcgcga cctcggcccg cacgtggggc
      181 aggtgctcgc gaaagatccg gtagtaggcc agctcctcct tggtgcgcag cggcgggtcc
      241 accgcgtgga actccgcgtc gaactcggcg tcgatgatgt ccgccgcgac cgcctcgctg
      301 agcacgtcgc gggcgccgct gccgtcgccg aactcggcct tgtcgcgcca gagcaggtcg
      361 tgcgggagcc agccctcgaa cgcctcgcgc agcagcgtct tctccggcgt gccgggggga
      421 gcgagcttgg cctcgggcgg gagcgccatc gcccagtcga tcaccgcccg gtcgagaaac
      481 gggacgcgcg cctcgaggcc gtgcgccatc gtcacgcggt cgcagcgctg gaggttgagg
      541 ttgtgcagac tgcaaatcgt gcgctcgagc tcggcgtgca gggcgccggc gtcggtgaag
      601 tcgcgcatgt aggcgtagcc ggcgaacagc tcgtcggcgc cctcgccagt tagcacgacc
      661 ttcacgtgct gcgcggtgaa gcgcgcgagc acgtagttcg gcacggcgct gcgtacgagg
      721 cccgggtcga acgactcgat cgagcgcacg acgccgggga gggcctcgag cgcgtcggcg
      781 gcggagtagg tcgtctcgtg gtgctcgcag ccgaggtgct cggccacgcg ccgcgcggcg
      841 gccaggtcgg gcgagccggg cgtgccgacc gcgaacgtct tcagccgctc gccgcgcgcg
      901 gcgaggatcc gggccgcgat ggcggcgacc aggctggagt cgagcccgcc cgagaggaac
      961 accccgaccg gcacgtcgcc catgagctgg cggtgcaccg actcgatcag cacgtcgcgc
     1021 gtgccgctca cgacgcggtg ggccgggacc ggttcggggt cctcgggcag cgcccgcgcg
     1081 aagcggacca ggccggtctc cggcgtccac gcgcagccgg gcgggaacgg ctcgaccgcg
     1141 ggctgccagc tcgggtcgaa cacgtgcatc tcggaggcga agagcacctc gtcgccgcgg
     1201 cgcgcccagt acagcggctt gatgccgacg ggatcgcgcg cggcgaggaa gcgcccgtcg
     1261 cggcccgcgg ccacgaaggc gaacatgccg ttcagctcgg ccagcgactc cggcccgcgc
     1321 tcgtcgagca ggtggagagc gacctcgttg tcggagctcg tcaggaacgg gcggtcggcc
     1381 agcccgtcgc gcacggcctc gtggttgtag acctcgccgt tgccgacgag gaacagctcg
     1441 ccgtcgggat cgtgcagcgg ctgggcgccg cctccgacgt cgacgatcga caggcgccgg
     1501 tgcccgagcc agctcccgtt gaccgcgacc gatccgctgt cgtcggggcc gcggtgcgcg
     1561 agccgggcga gcatggcctc gagcgctgcg cggtcggaga ccccgtgggc ggcgacgatt
     1621 ccgcacatgg tccgctccta cccccgcgcg gccgccggca aagcggccgc cgcaccggcc
     1681 acgtgcccgt acgcgtgcgc gcggcggtcg ccgaggtggt cgatgtgggc gcgcagggcc
     1741 ccgatctcgg tgcgggcatc caggtgagcc acggccaccc cggcgcgggc gccggtgcgg
     1801 gcgcgcacca cgccgtcggg gtccacgacc tgagcgcggc ccaggaagcg cagcgggccc
     1861 cacgggccgg actggttcga ggagacccag acgacctggt tctcgacggc ccgcgcggcc
     1921 gccatcaggt cgaagtggcg ggcctggcgg tcggccgacg agcgcgcagc ggggttgaga
     1981 cggtcgacgg gccaggaggc catgcaggcg atcgtctccg cgccgtccag cgcgagcgcg
     2041 cgggcggcct ccgggaagag cttgtcgtaa cagagcagca ttcccacccg tccggcgggg
     2101 gtgtcgaagg cggcaaaccc gtcgccggcc gagtaggcga agcgttcggc gggggggagg
     2161 tggaccttgc ggtggcggcc caggacgccg tcgccgctca cgcacaccgc gctgctgtag
     2221 ggaccgccgg gcgcggcctc ggtgtagccg atgcacacga ccgtctcgcc cgcgaggcgg
     2281 atcaggcgcg cgatctcggg cccgtcggga tcgagcgcgg ggggcaggtc gggcccgatc
     2341 tcgccgggca gcggctcgcg caggtagccg ccgagcgcgc actcggggaa cacgacgagg
     2401 cgcgcgccgc gccggcgcgc cgcgacgagc gtgcgctcga cgcgcgcgaa cgccgcatcg
     2461 atgtcgcgcc cgaatggcgc cgcgacggcc gcgatcgtgg gctcccgctc catgcgggga
     2521 tcttcggccc tacggctccc tgggcctacc gcccggcggt cggaatcggc cgggcggttt
     2581 tcgacatgcg gtcgagttag gaggtctcgg gccggctctc ggtcggtgcg tcgggggcgt
     2641 cgggggcgtc gcgcaccgtg cccgccagca gcttgtcgtg gtccgacacg agcgagtcga
     2701 gcctcacgcg gcgcaggttc atcccgctga gctcggcctg gaggcggttg ttgaacgtca
     2761 cgtgcagctc gccgccaccc tcgtagcggc cgaccatgtg ctggccgttg tagctgatca
     2821 gcactcccca ggtctcgtcc gtgtcgccgc cgatgtgctg atcggcgatg tcccggaagc
     2881 tgaccgagcc gaactcctcg ctgtggacgt acgtgtttcc gttgacgccg gacctccacg
     2941 tatcgccggc cgcgatggtc tgcccgtcct tggttgtcat cggcgcgtca gtcgagttga
     3001 cgatcgtgac gaaatagctc acttcagcgg cctcccgcga ttaaggatcc ttagcgtgag
     3061 cgatcctatt cgccggacgg cgcccggcgc aagagccgtc gtacgatcgc gccctgtgat
     3121 gcgcacgccg gtgcctcttc tcctcctcgt gatcgccgcc gctctggcgg cagccgggtg
     3181 tgggggtgac gacggcggcg gccctgcggc gcccgcgccg accacgagta cgcaggcgga
     3241 gccggctccg gcccccacga ccggggaggc cgagccgcgc gagtcagacg agccgaagcc
     3301 gcgcggcgag cgccgtgagc gcaccccgcg ctcgctggcc ggctgcatca aggacgcgcc
     3361 cggcgtcgag gacgcgctcg tgaaggggcg cgacagcgag gacgcgacgt tcttcgccga
     3421 cctcgtgggc gggcgtgtgg acgtgctcgg cgtgacgctg agcggcgagt cggccgaggt
     3481 cggcgtgttc ctgttcgcga cgccggcggc ggcggcgaag gccgcaccgc aggcgggcgg
     3541 cggcgggacg gtggcccgcg
//
LOCUS       DASVPL010000153        22188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_174934, whole genome shotgun sequence.
ACCESSION   DASVPL010000153 DASVPL010000000
VERSION     DASVPL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22188
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174934"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..560
                     /locus_tag="VF517_07895"
     CDS             33..560
                     /locus_tag="VF517_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052899.1"
                     /translation="MPFTDFLRTTVLLCSGAATALAVVAVAGARREDDITALYVSLGW
                     WALAALIGLWLGRRPEVTEGIGRLLAASRATQTLPELQPGTIVISRLWALALLTIGSG
                     AVAFLIPQVPAIAAGYCLLLALAWRRQSSAVEAVEQRDGVRFWVENGSPFKPTQLVRT
                     PGFRRNEPAHHDVRA"
     gene            complement(520..2292)
                     /gene="polX"
                     /locus_tag="VF517_07900"
     CDS             complement(520..2292)
                     /gene="polX"
                     /locus_tag="VF517_07900"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX8052900.1"
                     /translation="MRNAEIALHFDELADLYELDGAIVHRVLAYRNAAKAIRDAPASV
                     EEMTRAGTVTQLRGIGKTIEEKLNALLETGEIPSAAKLKAKFPPGLVEVTRIPGFGPK
                     KARKLFDELGVASLDDLRKAAEEGKLRDVRGFGEKAEETVLLALDAGVDLRPKPRLLL
                     SRALGIADALAEALREHPAAERVEVAGSARRQVEACRDLDLVAASEEPEELAAAFCEL
                     PLIGEVSSSAAAGVRAVTHNGLSIDLRIVQPSSFGNLLQHLTGSKSHNESLRTEAVKR
                     GLHVSEYGIADDSTGTTHACATEEEVYELLGMSWVPPELREDRGELQAARAGTLPELL
                     SLDDIRGELHCHTVASDGRNTIEEMAHAALERGYEYVAFTDHSASHGFGNDVPPDELR
                     RQIERIREADARIDGIRLLAGSEVNIHPDGSLDYEDDLLAELDWIVGSLHSAFRTPEK
                     EQTERMIRAMEHPLVDAIGHPTGRLIERREPYPLDIDAVADAAARTGTFLEINGNPDR
                     RDLNEINARRAVDRGATIVIDSDAHGVETLRNTGYGVATARRAWLTAADVANTRTWEE
                     LQKLPKQAARSSRRARRGAPARSA"
     gene            2313..3056
                     /locus_tag="VF517_07905"
     CDS             2313..3056
                     /locus_tag="VF517_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX8052901.1"
                     /translation="MPGTWLVLPTYNEAENIEAIVGAVLPRLAEAAPDHTLLVVDDSS
                     PDGTGVIADRLAIEHESVEVLHRAGKEGLGRAYIAGFRRALDAGAELLMEMDADFSHD
                     PADVPRLIEAASGTDLVLGSRYVPGGGVEGWELHRRLLSRGGCWYARTILAVPVRDLT
                     GGFKCFRREVLEKIDLDDVQADGYGFQIEMTYRTLRAGLRVKEIPIVFHERRAGASKM
                     DARIAAEAVWKVPALRRRLSGHPRLGHSA"
     gene            3164..3493
                     /gene="trxA"
                     /locus_tag="VF517_07910"
     CDS             3164..3493
                     /gene="trxA"
                     /locus_tag="VF517_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX8052902.1"
                     /translation="MAGAITDVTDSNFQAEVIESSEPVLVDFWAPWCGPCRVIAPALE
                     EITEERDDLRIVKLNVDDNQQTAARYNVLSIPTLILFRNGEVAKQIIGAMPKKKLVQE
                     IEPALAV"
     gene            3502..3681
                     /locus_tag="VF517_07915"
     CDS             3502..3681
                     /locus_tag="VF517_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX8052903.1"
                     /translation="MPIVTIEWYEGRSPEQKKELAEKLTDLMVDVGKTQRDHVWIRFV
                     DSPKSEWAMGGEVQG"
     gene            complement(3678..3959)
                     /locus_tag="VF517_07920"
     CDS             complement(3678..3959)
                     /locus_tag="VF517_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052904.1"
                     /translation="MGAGLAGERGDPLGLVAMAFYGVLFVVGALRWDELVAWGKEHPV
                     ADRIVLAPLVFFAAALVTDLPLAVCGVIAVIAAAALVLVDLGRRRGTAT"
     gene            complement(4009..7098)
                     /gene="ileS"
                     /locus_tag="VF517_07925"
     CDS             complement(4009..7098)
                     /gene="ileS"
                     /locus_tag="VF517_07925"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX8052905.1"
                     /translation="MSPFRPVDPGQSFPQLEEVVLARWRERDVFRESIRRREGAERFV
                     FYEGPPTANGRPGSHHVLSRVFKDVFPRYKTMRGYQVPRKAGWDCHGLPVELEIEREL
                     GLSSKHEIEDYGVAEFNARCRDSVLTYIDEWNRLTERIGFWIDTGDAYFTLTNDYVES
                     VWWSLHEVWRRGLLYRGHKVVPYCPRCGTALSSHEVAQGYRDVVDPSVYVRFPVRGEE
                     GVSLLGWTTTPWTLVSNAALAVAPDVTYVRARVDGEVLIVAEPLVERVLGEGAAIESR
                     VAGRDLAGLSYEPPFPWISDYGEKGHTVLEADFVTTEDGTGIVHTAIAFGEDDFQLGQ
                     RYGLKVQNPVREDGTFDERVEAFAGKFVKDADPEIVEALRESGRLFRDEAYEHSYPHC
                     WRCGTPLLYYAKASWYVKTTAVRDEMLAENEKITWYPEHIKHGRFGDWLANNVDWALS
                     RERYWGTPLPVWTCGNGHELCVGSRAELERLAGEVPEDLHKPYIDEVAFPCGECGEEM
                     RRVPEVIDAWWDSGSMPFAQWHAPFENDSPFAERFPADYICEALDQTRGWFYSLLAVS
                     TLLYGQSSYRTVLCLGLILDPAGQKMSKSRGNVVVPWEVIEEHGADAFRWYYFTSKQP
                     WDGYRFSLETVGESVRQFMLTLWNTYGFLTLYGNVNGIERFDGGAADPHPLDRWALSR
                     LEATTDTVTERLDDYDTTSAGRAIGAFVEDLSNWYVRRSRRRFWEGDPSAFATLHRCL
                     VEVSKLLAPLTPFIADEIYDNLDGSEPSVHLCDFPVAGERDLELERDVSVLREAIELG
                     RAARAHAKVKVRQPLAEAIVVAAGEERAAIERFADQLLEELNVKRVRFVSEAEELGRW
                     ELKPNYRTLGPRFGKQMPQAAAAVAALDAQSVAATLRDGGTVGLAIDGHDHELTADDV
                     QLVLQPLEGYQVERAGSHAVALNLELDDALRREGLAREAVRAIQEARKAAGLDVSDRI
                     RLTLAGDAELIAAVREHEEYVAGETLAVEVNYRDVALTGDPVTIEGRKLWIGVERAT"
     gene            complement(7130..8011)
                     /locus_tag="VF517_07930"
     CDS             complement(7130..8011)
                     /locus_tag="VF517_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8052906.1"
                     /translation="MSGIRTNAAAELLGVSPNTLRSWERRFGYPAPRRTAGGHRQYEL
                     NELEALRRALLETHNISSAIQVARQRGEGLGSASRMVEAFDRFDEHLADRLMEESMSL
                     RSIERTVEELLLPGLELAEQRDAREAEFELACRWATGWLHAARRVTPPATRAEGILLF
                     DSSPALGMEGIQVQALELGLRRAGFRVLLLSVGLAQERMTRAMRALEPTALVLAGSGA
                     TLDVLGRLVYAVRQTGSSAPLFEYRGSMPVSGSHNIPTLGASPSDAVRNLKAMVDGPA
                     WGRVGAAPVGMRSVGRS"
     gene            complement(8138..8644)
                     /locus_tag="VF517_07935"
     CDS             complement(8138..8644)
                     /locus_tag="VF517_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8052907.1"
                     /translation="MRDLAQVAHRSRAQGSAEQVELSRFCTHCGSLFDAGGATPERPL
                     RGRVCSRCGLGMVLTCASELLREPGAAFLVVTSDLRISAASEAAEELFGTEQGLHGRP
                     LLSILTSPAGVADLARLVARAAMGERSVERMPVESAARRLKGVSLEAVIGTCGSPSAA
                     LVVVEGWT"
     gene            8840..9205
                     /locus_tag="VF517_07940"
     CDS             8840..9205
                     /locus_tag="VF517_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052908.1"
                     /translation="MGCKEGPAAIRTALARAPARVELSGTPLSACFTRGSNSADLQQV
                     GAAYVAVASGLSAEAKEQPDGPGALRLGYLVGAARRGATETQGIHEELMRRLEQELIG
                     VDTRTPSYRRGFEAGRRRG"
     gene            9216..10550
                     /locus_tag="VF517_07945"
     CDS             9216..10550
                     /locus_tag="VF517_07945"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX8052909.1"
                     /translation="MTSEQQLWGGETSKAVENFPVSGERVPPSVIRWLGRLKAAAART
                     NAELGLLDADVAERIGAAGDEIAQGRHDDQFPIDVFQTGSGTSSNMNANEVIAALAGE
                     GVHPNDHVNMGQSSNDVFPSAVHLAALAEATDQLIPALERLQGALAAKADEFADVVKS
                     GRTHLMDAVPVTLGQEFGGYAAQIRLGRDRVNSALPRVGQIPLGGTATGTGLNTHPDF
                     AAGVRAKLTEATSLPIDGPADPFEAQANRDSLVELSGALKVVAVSLTKIANDLALMGS
                     GPRTGLGEIALPELQKGSSIMPGKVNPVIPEVVLQVAAQVMGNDTAITVAGSQGQFEL
                     NVRVPMIARNLLHSIGLLSSASLLLTEKCVEGIEANHEALTRHAESTPAIATALNPYI
                     GYDRATEIVKEAVASRRTIREVALEKGIDEETLDRALDLHAMARGAKPAPAG"
     gene            10666..11883
                     /locus_tag="VF517_07950"
     CDS             10666..11883
                     /locus_tag="VF517_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX8052910.1"
                     /translation="MADVSDFGSDLAAPAALQANHGADTSYWNIGRGVYTAPVEGQVT
                     IVKLKGAVLPNERLRNTDAEKLAQIIHFQVLRPKGDGNLKLVERSTGHLEMPIADSQQ
                     AQELVTSYRPVNLCVKRGDIIAFNTIGAHEYRRDPGTPGGPGGAEYQVFGRVPDQASQ
                     WYEKDAGLNEGTTIDPSPFPPMIGYELLMRNTLASGPDASDICPGGYSQHIFRGLEFL
                     DHAQPTVKTKTSSIRLKIFCHGENYGSCSGNLGVDATLDGTPTHLGDLKFNVPPTHTQ
                     NVDVPVSRANLLKLQLAKSVVGTLTADARDNPRADDRVKWDSVPVQEKTTTDQVTLTP
                     DKPPCVVPQKLIGKSSRSAKAAVRKAGCTAVIKYQRTAKAKEIAKVVKVAPAAGTLLP
                     TASKVTLTIGKRK"
     gene            complement(11893..12276)
                     /locus_tag="VF517_07955"
     CDS             complement(11893..12276)
                     /locus_tag="VF517_07955"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8052911.1"
                     /translation="MLRYRAHSRASAAQLWPLLARPERWHEWAPHVRGAWGLGHPEVQ
                     ARATGAARLLGFVPVPAVIVRKSPGRSWAWRVGPVEMDHRLVPQRRGTAVEVTLKAPA
                     ALELALRATYGPLMMLALRRLAARP"
     gene            complement(12282..12467)
                     /locus_tag="VF517_07960"
     CDS             complement(12282..12467)
                     /locus_tag="VF517_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052912.1"
                     /translation="MEYAPTVLIFLIVCAIGVGVLFVVARLTAEPDGEKNAPFAVDDE
                     TPLGDTDEHSDTLDRAA"
     gene            12545..13828
                     /locus_tag="VF517_07965"
     CDS             12545..13828
                     /locus_tag="VF517_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX8052913.1"
                     /translation="MRKLLVTVAAVAAVACAFPAAGMAEVSTFGSSLAAPANSIEPNG
                     AIHPAYGGDWFGADTAFWNTQLADGGQVTAPRDGQIVEVRVKGIAMQGPHPWDPSNPR
                     ALVHFQTIHPQPNGTALVELTSAGVDWPIGGDPQQITTFRPLNLCLHKGDYVDFNTWG
                     GHEWRWDRYGGIPLQVFSRVPGSSMNWYENDADTNNGDYLTGRTRDGVELLLQTVIAS
                     GPDASDICPGGYSQHIFRGLEIQPSPQTAVLRTRDRVAKIRTFCHGENYGACVGTMKL
                     TADIGGQEVELGRTDYRVENSYTVNLQVPLSADVVRGIQGAGSLHVSVVADSHDDPRA
                     DERVKWGAAIPVQSKVTTGEATLKPDKLLPLCSVPQVKGLKLNSAKKALVKAGCPVGK
                     VRYARGSKRGRVINQKPNRGKALDAGARVALTVAR"
     gene            13873..15300
                     /locus_tag="VF517_07970"
     CDS             13873..15300
                     /locus_tag="VF517_07970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8052914.1"
                     /translation="MSVASPSRAHPLRGLALPRVRFRDAAIAVSALAVALRGLAMAAY
                     APAFLGYPDTSFYVAVANEGASEDPFRPGGYPVFLRAVHAFGITGTVLIQHVLGLATA
                     MLLFLIVRRLGGSQWVALVPAAAVALCGSQVMIEHALLSEPLFAFLVAAALYACVRSL
                     DDGIGWAVAAGMLLGAAVPVRLVALGLIPVAAIWLVLASPRRRLLVGLAFTVVAASIP
                     AAWGAVNDFTRTGPYNFYGRVGPFADCHRFDPPSGTRMLCIKSPPDRRGSTYSFIFTG
                     GSPAVAYFGHPQGYPEPTKDDVDRIRRFARAAAIGQPLDYVRTVAKDSFRYLAPEVWR
                     PPPGDATQMPEELNQYFTSSGWTGTAVRRSRAYYGGGVHPTANEPLLEALRVYERGTR
                     FTGLAFVLVLLPALAAPFLLRGRARAGALLLLLCGLALLAVPVATIFYDYRFAIPALG
                     ALATAGALGAGEIAGRVRARQDGAA"
     gene            15297..15884
                     /locus_tag="VF517_07975"
     CDS             15297..15884
                     /locus_tag="VF517_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8052915.1"
                     /translation="MRPVFWFDFNSPFVYLAAERIDPLIPDAEWRPFAFPLMLGAQGR
                     LEEAMGRDPHAAIEAASGRIAERGLPPLALPAGWPAATWSLHPLRAAVYADEQGRMRE
                     FVRVAYRRFCAEGLVLSEVANLRAAAEAAGLDPDAVLDAIERPDIKQRVKDNTDAAIA
                     RGLPGIPTVEVGGELVWGDDRLDEAAALAERADGH"
     gene            15990..16811
                     /locus_tag="VF517_07980"
     CDS             15990..16811
                     /locus_tag="VF517_07980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8052916.1"
                     /translation="MADVLSQLAVAMTPADALARAKWAAGQLGLKLHDETADGFRGGS
                     EWWGAHQITVRVTASGSGTLVALNASTVKIGYTGRLKQVTQRFVDLMTASSESLSMPV
                     EAAQPLGDRDLLVEKMLSRMEARAEKEERVEQLPAQAVLGFYPLKDHGPLIGAPGQGT
                     HSRTAPPDNWQSDNAVDIKVPKGTPVVAVENGVIGDRIGPLNSRDPRMGGLRVYVESR
                     DGNEFYYAHLSKLAVKAGQRVEKGQVIGYSGSAAGVDHLHFAQRKGDPRTTLKSD"
     gene            16889..18814
                     /locus_tag="VF517_07985"
     CDS             16889..18814
                     /locus_tag="VF517_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052917.1"
                     /translation="MGVVRGIAAVVALLALALAPAVEGAKSKPRPRIAVNVLTLSQRE
                     IVKQRAVRVEVTPERTATVRLRALVRRTSKRSDDISRTRELRIKAGKRRIVTLPVSKS
                     ALALFRTCKVVKVTISARARGLAPHRAAAPSRRLPPYVRCPGAPDPGPLPPLTYSFGL
                     ASRSINPDPDGRWKGEPVFLGGYGIGGGTPFLSGRAATGILAEGPQVRAFAVGDGKNT
                     IAVGDIEVQGWFVANKDAPYGIVEMRKEVERRTGGALKAENVTIQSNHSHGGADAMGV
                     WGDSPLDYRRFMFEQAVDAVVEAFQKREPANLYYGAVDARDLLSNQFGDDPFNETSHN
                     DTMDSDLRVLQARDARDKPIATVLNFSAHSTVLGSSNTRITGDWSQRANRMLAERFGG
                     KAMTMIGTFGRSQPNDRGCATKEKADQKVEADAVCVLDSYAERVVDRAQEAVRRATLI
                     AGDPVVEARSYLIQDLAQNGVVYLGLGVAGDPVGVGINRSLTPPWVTGDLLGTVTSTL
                     RIGDVMISAVPGEIYPQIALKVRDTVKGLRGYMTAGLANDQLGYIIAPVEAYPDPVRR
                     TFFNSRGDEVDPISNDNYAFNVSHTLGERVICSLLRGAGEAFGKGAAFRDSYERCNLF
                     PNDLAFEHGTDTTFPSP"
     gene            complement(18825..19061)
                     /locus_tag="VF517_07990"
     CDS             complement(18825..19061)
                     /locus_tag="VF517_07990"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HEX8052918.1"
                     /translation="MGIGTSIVLIAVGAILKYAVTASVSGIDIQTVGVILMVAGIVGL
                     VLSLLWTAMARRRGVVVDDVPRRGVLVDDRPRRF"
     gene            19240..19890
                     /locus_tag="VF517_07995"
     CDS             19240..19890
                     /locus_tag="VF517_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052919.1"
                     /translation="MTTRTRLSLILAATFVLAAVPAGDASAASCSAKGYKTLDQNKYI
                     VVQRGPGPKGDSTSDYGVYACRISTGKRRVLGGNECFGNDLGEVGDYALASNYIAYEA
                     RTCGDPEGESDAVSLNVATGAKKRFSAEHEPLAGGVVDYSITSLKVNSKGNMAWIAQA
                     KHLSGGNQTRFDVRRAINGVADRVDVGPGIVGDSLAYSAGRIYWLNGDVPKVAALP"
     gene            complement(20129..22042)
                     /locus_tag="VF517_08000"
     CDS             complement(20129..22042)
                     /locus_tag="VF517_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8052920.1"
                     /translation="MHLLSAGLWGYRRFRDQQPNMDVGARVVAIVGPNEAGKTSFLRA
                     LLHLNASGPFGAADYTRGSDGRVCVRAIFGLNAEDREAVADVDPDRRIRTFEIWKRES
                     GILYGRVEPEMERDRAPRRHARDALARLAGQKWARSAEGFDRIENLARSALDAEEETL
                     PNEAIDELETCAGYLREYEDAPPTAIRLADELQQLANYERRPEPGEESARRLLERRPE
                     FLFFADEHRELAPVHLISETPTIALENFLALAQLDLQRLRLAVEASDEATVEELEVAA
                     NARLSEMFERYWSQANLTVRLRVTAGELHILVSSAPGRFISIAERSDGLRQFVALASY
                     VERHASRNNLVLLIDEAETHLHYDAQADLIKVLTNQAVAGKVIYSTHSAGCLPEDLGA
                     GVRLVTPTGPDDTDPAEWQHSRVLNRIWSSDVQGGAFSPLLIAMGAGTFAFTAARFAL
                     VVEGITDAIMLPTLLRQAVGAEKLGFQVAPGIAEAAPEALAQLDLTAARVVYLVDSDP
                     DGDRHCTRLVTAGVQAARILRLGRTNQGLSLEDLISRDIYLAAVQAELRERHGLDIPA
                     PEIPDIGRARAVANWCKKQSVDGSQIELSKRAVSQRVLEQRFDHTIVAKKRLDLLRTL
                     HSRISALLEVEAL"
BASE COUNT         3379 a         7786 c         7835 g         3188 t
ORIGIN      
        1 gacgtcctcg agcgcctcgc ggccgtgcgc tagtgccctt caccgacttc ctgcgcacca
       61 ccgtgctcct gtgcagcggc gccgccacgg cgctcgcggt ggtcgccgtc gccggcgcgc
      121 gccgcgagga cgacatcacg gcgctctacg tctcgctcgg ctggtgggcg ctcgccgcgc
      181 tgatcggcct gtggctcggg cgccggccgg aggtgacgga gggcatcggc cggctgctcg
      241 cggcctcgcg cgcgacgcag acgctgcccg agctccagcc cggcacgatc gtgatcagcc
      301 gcctgtgggc gctcgcgctg ctgacgatcg gctccggcgc ggtggccttc ctcatcccgc
      361 aggtccccgc gatagcggcc ggctactgcc tgctcctcgc gctggcctgg cggcggcagt
      421 cgagcgccgt agaggcggtc gagcagcgcg acggcgtgcg cttctgggtg gagaacggct
      481 cgccgttcaa gcccacccag ctcgtgcgca ccccgggctt caggcggaac gagccggcgc
      541 accacgacgt gcgcgcctag agctccgggc cgcctgcttc ggcagcttct ggagctcttc
      601 ccatgtgcgc gtgttcgcaa cgtccgcggc ggtgagccag gcgcggcggg cggtcgccac
      661 gccgtagccg gtgttgcgca gcgtctcgac gccgtgcgcg tcggagtcga tcacgatcgt
      721 cgcgccgcgg tccacggcgc ggcgcgcgtt gatctcgttc aggtcgcgcc ggtccgggtt
      781 gccgttgatc tcgaggaagg tgcccgtgcg cgctgcagcg tcggccacgg cgtcgatgtc
      841 gagcgggtac ggctcgcggc gctcgatcag ccggccggtc gggtgcccga tcgcgtcgac
      901 cagcgggtgc tccatcgcgc ggatcatgcg ctcggtctgc tccttctccg gcgtgcggaa
      961 cgcggagtgg aggctcccga cgatccagtc cagctctgcc agcaggtcgt cctcgtagtc
     1021 gagcgagccg tcggggtgga tgttcacctc cgagccggcc agcagccgga tgccgtcgat
     1081 ccgcgcgtcg gcctcgcgga tgcgctcgat ctggcggcgc agctcgtcgg gcggcacgtc
     1141 gttgccgaag ccgtgcgagg cggagtggtc ggtgaacgcg acgtactcgt agccgcgctc
     1201 gagcgccgcg tgtgccatct cctcgatcgt gttgcgcccg tccgaggcga cggtgtggca
     1261 gtgcagctcg ccgcgtatgt cgtcgaggct cagcagctcg ggcagcgtgc cggcgcgggc
     1321 cgcctggagc tcgccgcggt cctcgcgcag ctccggcggg acccagctca tcccgagcag
     1381 ctcgtagacc tcctcctcgg tcgcgcacgc gtgcgtcgtc ccggtcgagt cgtcggcgat
     1441 tccgtactcg ctcacgtgca ggccgcgctt gaccgcctcg gtgcgaagcg actcgttgtg
     1501 gctcttcgac ccggtcaggt gctggagcag gttgccgaag ctcgagggct gcacgatccg
     1561 taggtcgatg ctgaggccgt tgtgggtcac ggcccgcacg cccgcggccg ccgacgagct
     1621 gacctcgccg atcagcggca gctcgcagaa cgcggcggcg agctcttcgg gctcctcgct
     1681 ggcggccacg aggtcgaggt cgcggcacgc ctccacctgg cgccgcgcgc tgccggccac
     1741 ctccacgcgc tcggcggcgg gatgctcgcg cagcgcctcg gccagcgcgt cggcgatccc
     1801 gagcgcgcgc gagagcagca gccgcggctt gggccgcagg tccacgccgg cgtcgagcgc
     1861 gagcagcacg gtctcctcgg ccttctcgcc gaacccgcgc acatcgcgca gcttgccctc
     1921 ctcggccgcc ttgcgcaggt cgtcgagcga cgcgacgccg agctcgtcga agagcttgcg
     1981 cgccttcttc ggcccgaagc cggggatccg cgtgacctcc acgaggccgg gcgggaactt
     2041 ggccttcagc ttcgccgccg acgggatctc cccggtctcg agcagcgcgt tcagcttctc
     2101 ctcgatggtc ttgccgatgc cgcgcagctg cgtcaccgtg cccgcgcgcg tcatctcctc
     2161 gacggaggcg ggcgcgtcgc ggatcgcctt ggcggcgttg cggtaggcga gcacgcggtg
     2221 gacgatcgcc ccgtcgagct cgtagaggtc ggcgagctcg tcgaagtgga gggcgatctc
     2281 ggcgttccgc atgcccggtc tagggtacgg gcgtgcccgg cacctggctt gtgctcccga
     2341 cgtacaacga ggccgagaac atcgaggcga tcgtgggggc ggtgctaccg cgcctggccg
     2401 aggccgcgcc ggatcacacg ctgctcgtgg tggacgactc ctcgcccgac ggcacgggcg
     2461 tgatcgccga ccggctcgcg atcgagcacg agtccgtcga ggtgctccac cgcgccggca
     2521 aggagggcct cggccgcgcg tacatcgcgg ggttccgccg cgcgctcgac gcgggtgcgg
     2581 agctgctgat ggagatggac gccgacttct cgcacgaccc ggccgacgtg ccgcggctga
     2641 tcgaggcggc gtcgggcacc gacctggtgc tcggctcgcg ctacgtcccc ggcggcgggg
     2701 tcgagggctg ggagctgcac cgccggctgc tctcgcgcgg cggctgctgg tacgcgcgca
     2761 cgatcctcgc cgtgccggtg cgcgacctga ccggcggctt taagtgcttc cgccgcgaag
     2821 tgctcgagaa gatcgacctc gacgacgtgc aggccgacgg ctacggcttc cagatcgaga
     2881 tgacctaccg cacgctcagg gcgggcctgc gcgtgaagga gatcccgatc gtgtttcacg
     2941 agcgccgcgc cggtgcgtcg aagatggacg cgcggatcgc tgccgaggcg gtgtggaagg
     3001 tgcccgcgct gcgacggcgg ttgagcggtc acccacggct cggccactcc gcgtaagctg
     3061 ccgtcagcag gcctcgattc gggcctgccg ggggtcccga cccggcttgc cgcgggtcac
     3121 tatacttttt gaagcaaggc gagcaaccga acggagcaga ccgatggcag gagcgataac
     3181 ggacgtcacc gactcgaact tccaggccga ggtgatcgag tccagcgagc ccgtgctcgt
     3241 cgacttctgg gctccctggt gcgggccgtg ccgcgtgatt gcgccagcgc tcgaggagat
     3301 caccgaggag cgcgacgacc tgcggatcgt gaagctgaac gtcgacgaca accagcagac
     3361 cgcggcgcgc tacaacgtgc tgtcgatccc cacgctgatc ctcttccgca acggcgaggt
     3421 cgccaagcag atcatcggcg cgatgccgaa gaagaagctc gtgcaggaga tcgagccggc
     3481 gctggccgtc tagccggcgc catgccgatc gtcacgatcg agtggtacga gggccgttcg
     3541 cccgagcaga agaaggagct ggccgagaag ctgaccgacc tgatggtcga cgtcggcaag
     3601 acccagcgcg accacgtctg gatccgcttc gtcgactcgc cgaagagcga gtgggcgatg
     3661 ggcggcgagg tccagggcta ggtcgccgtc ccgcggcgcc gtcccaagtc caccagcacg
     3721 agggccgcgg cggcgatcac cgcgatcacg ccgcagacgg caagcggtag gtcggtgacg
     3781 agagccgccg cgaagaacac gagcggcgcc aacacgatcc ggtcggccac cgggtgctcc
     3841 ttgccccacg ccaccagctc gtcccacctg agcgcgccga cgacgaagag cacgccgtag
     3901 aacgccatcg cgacgagccc gagcggatcc ccgcgctcgc cggccagccc ggcgcccatc
     3961 agcacagcga gggaaacgcc caggatggcg ataccggcgc gtcccgctct acgtcgcacg
     4021 ctccacgccg atccagagct tccgcccctc gatcgtcacc gggtcgcccg tgagcgccac
     4081 gtcgcggtag ttcacctcca cagcgagcgt ctcgcccgcc acgtactcct cgtgctcgcg
     4141 caccgccgcg atcagctcgg cgtcgcccgc cagcgtcagc cggatccggt ccgacacgtc
     4201 gagcccggcc gccttgcgcg cctcctggat cgctcgcacc gcctcgcggg ccagaccctc
     4261 gcgccgcagc gcgtcgtcca gctccaggtt cagcgccacc gcgtgcgagc cggcacgctc
     4321 cacctggtag ccctcgagcg gctggagcac gagctgcacg tcgtcggcgg tcagctcgtg
     4381 gtcgtggccg tcgatcgcga gcccgaccgt cccgccgtcg cgcagcgtgg ccgccaccga
     4441 ctgggcgtcc agagcggcca ccgccgcggc cgcctgcggc atctgcttgc cgaagcgggg
     4501 accgagcgtg cggtagttcg gcttcagctc ccagcgccct agctcctcgg cctcggagac
     4561 gaaccgcacc cgcttcacgt tcagctcctc gaggagctga tcggcgaagc gctcgatggc
     4621 cgcgcgctcc tcccccgccg cgaccacgat cgcctcggcc agcggctggc gcaccttcac
     4681 cttcgcgtgc gcgcgcgccg cgcgcccgag ctcgatcgcc tcgcgcagca cgctcacgtc
     4741 gcgctccagc tcgaggtcgc gctcgcccgc aaccgggaag tcgcacaggt gcaccgacgg
     4801 ctcggagccg tcgaggttgt cgtagatctc gtccgcgatg aacggggtga gcggggcgag
     4861 cagcttcgag acctcgacca ggcagcgatg gagcgtggcg aacgcgctcg ggtcgccctc
     4921 ccagaagcgc cgccgcgagc gccgcacgta ccagttcgac aggtcctcca cgaaggcgcc
     4981 gatcgcccgc ccggcgctcg tggtgtcgta gtcgtcgagc cgctcggtga ccgtgtcggt
     5041 cgtggcctcc agcctcgaca gagcccagcg gtcgagcggg tgcggatccg ccgcgccgcc
     5101 gtcgaaccgc tcgatcccgt tcacgttccc gtacagcgtg aggaacccgt acgtgttcca
     5161 cagcgtgagc atgaactggc gcacgctctc gccgaccgtc tcgagcgaga agcgatagcc
     5221 gtcccacggc tgcttcgagg tgaagtagta ccagcggaac gcgtccgcgc cgtgctcctc
     5281 gatcacctcc cagggcacca ccacgttgcc gcgcgacttc gacatcttct gcccggcggg
     5341 gtcgaggatc agcccgaggc acagcaccgt gcggtacgag ctctggccgt acagcagcgt
     5401 ggacaccgcg agcagcgagt agaaccaccc gcgcgtctgg tcgagcgcct cgcagatgta
     5461 gtccgcgggg aaccgctccg cgaaggggga gtcgttctcg aacggcgcgt gccactgcgc
     5521 gaacggcatc gagccggagt cccaccacgc gtcgatcacc tcgggcacgc ggcgcatctc
     5581 ctcgccgcac tccccgcagg ggaacgcgac ctcgtcgatg tacggcttgt gcaggtcctc
     5641 gggcacctcg cccgccagcc gctcgagctc cgcgcgcgag ccgacgcaca gctcgtgccc
     5701 gttgccgcac gtccacaccg gcagcggcgt gccccagtag cgctcgcgcg agagcgccca
     5761 gtccacgttg ttggcgagcc agtcgccgaa gcgcccgtgc ttgatgtgct ccgggtacca
     5821 ggtgatcttc tcgttctcgg cgagcatctc gtcgcggacc gcggtcgtct tcacgtacca
     5881 gctcgccttg gcgtagtaga ggagcggcgt gccgcagcgc cagcagtgcg gataggagtg
     5941 ctcgtaggcc tcgtcgcgga agaggcggcc ggactcgcgc agcgcctcga cgatctccgg
     6001 gtccgcgtcc ttcacgaact tgcccgcgaa cgcctccacg cgctcgtcga aggtgccgtc
     6061 ctcgcgcacc gggttctgga ccttcagccc gtagcgctgg ccgagctgga agtcgtcctc
     6121 gccgaacgcg atagccgtgt gcacgatccc ggtgccgtcc tcggtggtca cgaagtcggc
     6181 ctcgagcacg gtgtggccct tctccccgta gtcggagatc caggggaacg gcggctcgta
     6241 ggagagcccg gcgaggtcgc ggccggccac gcgcgactcg atcgcggcac cctcgccgag
     6301 cacccgctcg accagcggct cggcgacgat cagcacctcg ccgtccaccc gcgcacgcac
     6361 gtaggtcacg tccggcgcca cggccagggc cgcgttcgac acgagcgtcc acggcgtggt
     6421 ggtccagccg agcagcgaca cgccctcctc gccgcgcacc gggaagcgca cgtacacgct
     6481 cggatcgacg acgtcgcggt acccctgtgc gacctcgtgc gacgacagcg ccgtgccgca
     6541 gcgcgggcag tacggcacga ccttgtggcc gcggtagagc agcccgcgcc gccagacctc
     6601 gtgcagcgac caccagaccg actcgacgta gtcgttggtc agcgtgaagt acgcgtcgcc
     6661 cgtgtcgatc cagaacccga tccgctcggt cagccggttc cactcgtcga tgtaggtgag
     6721 caccgagtcg cggcagcgcg cgttgaactc ggcgaccccg tagtcctcga tctcgtgctt
     6781 ggaggagagc cccagctcgc gctcgatctc gagctcgacc ggcaggccgt ggcaatccca
     6841 gcccgccttg cgcggcacct ggtagccgcg catcgtcttg tagcgcggga agacgtcctt
     6901 gaacacgcgc gagaggacgt ggtgcgagcc cggccgtccg ttggcggtcg gcggcccctc
     6961 gtagaagacg aaccgctcgg cgccctcgcg gcggcggatc gactcgcgga agacgtcgcg
     7021 ctcgcgccag cgcgcgagga cgacctcctc gagctgcggg aacgactggc ctgggtcgac
     7081 cgggcggaag gggctcacgg ggagccgatg ctatgacgcg gagacgcggc taggagcggc
     7141 cgacgctgcg catgcccacg ggcgccgcgc cgacgcggcc ccaggcggga ccgtcgacca
     7201 tcgccttgag gttgcgcacc gcgtcgctcg gcgaggcgcc gagcgtgggg atgttgtggc
     7261 tgccgctgac cggcatcgac ccgcggtact cgaacagcgg ggcggaggag ccggtctggc
     7321 ggacggcgta gacgaggcgg ccgagcacgt cgagcgtggc gccgctgccg gcgagcacga
     7381 gcgcggtcgg ttcgagtgcg cgcatcgcgc gggtcatccg ctcctgagcc aggccgaccg
     7441 agagcagaag cacgcggaag ccggcgcggc gcagccccag ctcgagggcc tgcacctgga
     7501 tgccctccat gccgagggcg gggctcgagt cgaagaggag gatgccctcg gcgcgcgtgg
     7561 cgggcggggt cacgcggcgg gcggcgtgca gccagcccgt agcccagcgg caggccagct
     7621 cgaactcggc ctcgcgggcg tcgcgttgct cggcgagctc gaggccgggg aggaggagct
     7681 cctcgaccgt gcgctcgatg gagcgcagcg acatgctctc ctccatcagg cggtcggcga
     7741 ggtgctcgtc gaagcggtcg aacgcctcga ccatgcgcga cgcggagccg aggccctcgc
     7801 cgcgctggcg ggcgacctgg atcgccgagg agatgttgtg cgtctcgagc agggcacggc
     7861 gcagcgcctc gagctcgttc agctcgtact ggcggtggcc accggccgtc cggcgcgggg
     7921 ccgggtagcc gaaacggcgc tcccagctcc ggagggtgtt ggggctgacg ccgagcagct
     7981 cggcggcagc atttgtgcgg atgccgctca tgtggtcacc ttcggcacga gacgcaggtt
     8041 cctttaaggg ttgggcgcgt gaagctccaa cccgccgcca gagttgtgac agctcggggt
     8101 gctcttgcgc aacctctgaa cgcgaagttg cacagcccta ggtccacccc tcgaccacga
     8161 cgagcgccgc ggacgggctc ccgcacgtcc cgatcacggc ctcgagcgag acccccttga
     8221 gccgccgcgc ggccgactcg accggcatcc gctcgaccga gcgctcgccc atcgcggccc
     8281 gcgcgaccag ccgggcgagg tcggccacgc ccgccgggct cgtgaggatg gacaggagcg
     8341 ggcggccgtg gagcccctgc tcggtcccga acagctcctc ggccgcctcg gacgctgcgc
     8401 tgatccgcag gtcggaggtg acgacgagga acgccgcgcc gggctcgcgc agcagctcac
     8461 tcgcgcacgt gagcaccatg ccgagcccgc agcgcgagca cacgcgaccg cgcagcggcc
     8521 gttcgggtgt tgcaccgccg gcgtcgaaca gcgagccgca gtgcgtgcag aatcgggaca
     8581 gctcgacctg ctcggcgctt ccttgcgctc gagaccggtg agcgacctgg gccagatctc
     8641 tcatcctcgt tgctccttcg gtgatggggg gagtccgttc cccgcgaaga cgagaagaac
     8701 atgcgaaggt gccgttcgca tgggggtgca ggttttgcag cggatgcctt ccggcaaacc
     8761 gggcaaagct ccaatctgtc tggcaatcgt gcttgcactg ggcgggtgcg ccaggcagag
     8821 cggggatccg ccggcgccgg tggggtgcaa ggaggggccg gccgcgattc gcaccgccct
     8881 cgcacgcgcg cccgcgcgcg tggagctcag cggcacgccg ctgtcggcgt gcttcacgcg
     8941 cggcagcaac tcggccgacc tccagcaggt cggcgccgcg tacgtcgcgg tcgcctcggg
     9001 gctgtccgcg gaggcgaagg agcaacccga cgggccgggt gccctgcggc tcggctatct
     9061 cgtcggcgca gcccgccgcg gcgccacgga gacccagggg atccacgagg agctgatgcg
     9121 ccggctcgag caggagctga tcggggtcga cacgcgaacg ccctcctatc ggcggggctt
     9181 cgaggcgggg cgcaggcgtg gatagggttg aacccatgac ctccgaacag cagctctggg
     9241 gcggcgagac gagcaaggcc gtcgagaact tcccggtgtc cggcgagcgc gtgccgccgt
     9301 cggtcatccg ctggctcggc cgcctcaagg ccgccgccgc gcgcaccaac gccgagctcg
     9361 gcctgctgga cgcggacgtc gccgagcgga tcggcgccgc gggcgacgag atcgcccagg
     9421 gccgccacga cgaccagttc ccgatcgacg tcttccagac cggctcgggc acgtcctcga
     9481 acatgaacgc gaacgaggtg atcgccgcac tcgcgggcga gggcgtccac ccgaacgacc
     9541 acgtgaacat ggggcagtcg tcgaacgacg tgttcccgtc ggccgtccac ctggccgccc
     9601 tggcggaggc gaccgaccag ctcatccccg ccctcgagcg cctccagggt gccctcgccg
     9661 cgaaggccga cgagttcgcc gacgtcgtga agtccggccg cacgcacctc atggacgcgg
     9721 tgccggtcac gctcggccag gagttcggcg gctacgcggc gcagatccgg ctcggccgcg
     9781 accgcgtgaa ctccgccctg ccgcgcgtgg gccagatccc gctcggcggc acggccaccg
     9841 gcaccgggct caacacgcat ccggacttcg ccgcgggcgt ccgcgcaaag ctcaccgagg
     9901 cgaccagcct gccgatcgac ggccccgccg acccgttcga ggctcaggcc aaccgcgact
     9961 ccctcgtgga gctctccggc gcgctcaagg tcgtggccgt ctcgctcacc aagatcgcca
    10021 acgacctggc gctgatgggc tccggcccgc gaaccggcct cggcgagatc gcgctgcccg
    10081 agctccagaa gggctcgtcg atcatgcccg gcaaggtcaa cccggtcatc ccggaggtcg
    10141 tcctccaggt ggccgcccag gtgatgggca acgacaccgc gatcaccgtc gccggctcgc
    10201 agggccagtt cgagctgaac gtgcgcgtgc cgatgatcgc tcgcaacctg ctccactcga
    10261 tcgggctgct gtcgagcgcg tcgctgctcc tgaccgagaa gtgcgtcgag ggcatcgagg
    10321 cgaaccacga ggcgctcacg cgccacgccg agtccacgcc cgcgatcgcg accgcactga
    10381 acccgtacat cggctacgac cgcgccaccg agatcgtgaa ggaggcggtc gcctcgcggc
    10441 gcacgatccg cgaggtggcg ctcgagaagg gcatcgacga ggagacactc gaccgcgccc
    10501 tcgacctcca cgcgatggca cgcggcgcca agcccgcgcc ggcgggctag cgaaaagcgc
    10561 tcggcggtcc tacgctgggc ggagcggcag gcccgccgcc gactcgccat gcgccgcctc
    10621 ttcctcgtag ctctcgcagc gacatgcctg gctcccgcca gcgctatggc ggacgtgtcg
    10681 gacttcggca gcgacctggc cgcccccgcc gcactccagg ccaaccacgg cgccgacacc
    10741 tcgtactgga acatcggccg cggcgtctac acggcgccgg tggagggcca ggtgacgatc
    10801 gtcaagctca agggggccgt gctcccgaac gagcgcctgc gcaacaccga cgcggagaag
    10861 ctcgcgcaga tcatccactt ccaggtcctg cgcccgaagg gcgacgggaa cctgaagctc
    10921 gtcgagcgca gcacgggcca cctcgagatg ccgatcgccg actcgcagca ggcccaggag
    10981 ctcgtcacct cgtaccgtcc ggtcaacctc tgcgtcaagc gcggcgacat catcgcgttc
    11041 aacacgatcg gcgcccacga gtacaggcgc gatccgggca cgcccggtgg cccgggtggt
    11101 gccgagtacc aggtgttcgg ccgcgtcccg gaccaggcgt cccagtggta cgagaaggac
    11161 gcggggctga acgagggcac cacgatcgat ccctcgccgt tcccgccgat gatcggctac
    11221 gagctgctga tgcggaacac gctcgccagc gggccggacg cgtccgacat ctgccccggc
    11281 ggctactcgc agcacatctt ccgcgggctc gagttcctgg accacgcgca gccgacggtc
    11341 aagaccaaga cgagctcgat ccgcctcaag atcttctgcc acggggagaa ctacgggagc
    11401 tgctcgggca acctcggcgt ggacgccacg ctcgacggca cgccgaccca cctcggcgac
    11461 ctcaagttca acgtaccgcc cacccacacg cagaacgtcg acgtgccggt gtcccgggcg
    11521 aacctgctga agctccagct cgcgaagagc gtggtgggca cgctgacggc ggacgcgcgc
    11581 gacaacccgc gtgccgacga ccgggtgaag tgggacagcg tgccggtgca ggagaagacg
    11641 acgaccgatc aggtcacgct cacgccggac aagccgccgt gcgtggtgcc gcagaagctg
    11701 atcggaaagt cgtcgaggag cgccaaggcg gcggtgcgca aggccggctg caccgcggtc
    11761 atcaagtacc agcggacggc caaggcgaag gagatcgcca aggtcgtgaa ggtcgcgccc
    11821 gcggcgggga cgctgctgcc caccgccagc aaggtcacgc tgacgatcgg caagcggaag
    11881 tagcgacggc tcttaggggc gcgcggccag gcgccgcagc gccagcatca tcagcgggcc
    11941 gtaggtggcc cgcagcgcca actcgagtgc cgcgggcgcc ttcagcgtga cttccacggc
    12001 cgtgccgcgt cgttgcggta cgagccgatg gtccatctct accggtccca cacgccacgc
    12061 ccacgagcgt cccgggctct tgcggacgat caccgcgggc acgggcacga agccgagcag
    12121 gcgggcggcg cccgtggcgc gcgcctgcac ctcgggatgg ccgagtcccc acgcgccgcg
    12181 cacgtgcgga gcccattcgt gccagcgctc gggccgggcc aggagcggcc agagctgcgc
    12241 ggcgctggcg cggctgtggg cgcggtagcg cagcacggcg ctcaggcagc ccggtcgagg
    12301 gtgtcggagt gctcgtcggt gtcgccgagc ggcgtctcgt cgtcgacggc gaacggcgcg
    12361 ttcttctcgc cgtcgggctc ggcggtcagc cgggcgacga cgaacagcac ccccacgccg
    12421 atggcgcaga cgatcaggaa gatcaggacg gtcggtgcgt actccacggt ctaggtacgg
    12481 gcgcgggcgg ctcagggatt tcggccctcc gtccgatcta ggtagggcgg gcatgtctct
    12541 ctcggtgcgg aagctcttgg tcacggtcgc cgccgtagcg gcggtggcct gcgcgttccc
    12601 ggccgcgggc atggcggagg tctcgacctt cggcagctcg ctcgcggccc cggccaactc
    12661 gatcgagccg aacggcgcga tccatcccgc ctacggcggc gactggttcg gcgccgacac
    12721 tgccttctgg aacacccagc tggcggacgg cggccaggtc accgcgccgc gcgacggcca
    12781 gatcgtcgag gtccgcgtca agggcatcgc gatgcaaggt ccccacccgt gggacccgag
    12841 caatccccgc gcgctcgtcc acttccagac gatccatccg cagccgaacg ggaccgccct
    12901 ggtcgagctc acctccgccg gcgtcgactg gccgatcggc ggcgacccgc agcagatcac
    12961 caccttccgc ccgctcaacc tctgtctcca caagggcgac tacgtcgact tcaacacctg
    13021 gggcggccac gagtggcgct gggaccgcta cggcgggatc ccgctccagg tcttcagcag
    13081 ggttcccggc tcgtccatga actggtacga gaacgacgcc gacaccaaca acggcgacta
    13141 cctcacgggc cggacgcggg acggggtcga gctgctgctc cagaccgtga tcgccagcgg
    13201 tcccgacgcc tccgacatct gccccggcgg ctactcgcag cacatcttcc gcgggctcga
    13261 gatccagccc tcgccgcaga ccgcggtgct ccgcacgcgc gatcgcgtcg cgaagatccg
    13321 caccttctgc cacggcgaga actacggcgc ctgcgtcggc acgatgaagc tgaccgccga
    13381 catcggcggg caggaggtcg agctgggccg cacggactac agggtcgaga actcgtacac
    13441 ggtgaacctc caggtgccgt tgtcggccga cgtcgtgcgc ggcatccagg gggcgggttc
    13501 gctccacgtc agcgtggtcg ccgactcaca cgacgacccg cgtgccgacg agcgcgtcaa
    13561 gtggggcgcc gcgatcccgg tccagagcaa ggtcacgacc ggcgaggcca cgctcaagcc
    13621 ggacaagctg ctgccgctgt gcagcgtgcc ccaggtcaag gggctcaagc tgaactcggc
    13681 caagaaggcg ctcgtgaagg cgggctgccc ggtcggcaag gtgcgctacg cgcgtggctc
    13741 caagcgcggt cgcgtgatca accagaagcc gaaccgcggc aaggcgctcg acgccggcgc
    13801 gcgcgtcgcg ctcacggtcg cccgctaaca gccgcgcgaa tttcccgccg agtcgggtaa
    13861 gaggaccggc gtatgtcggt cgcatcgccg tcacgtgcgc atccgctccg gggtctggcg
    13921 ctcccgcgcg tgcgcttccg cgacgccgcg atcgcggtct cggcgctggc cgtcgcgctg
    13981 cgcggcctgg cgatggccgc gtacgcgccc gccttcctcg gctatccgga cacctcgttc
    14041 tacgtggcgg tggccaacga gggcgcttcc gaggacccgt tcaggccagg gggctacccg
    14101 gtcttcctga gggcggtcca cgcgttcggc atcaccggca cggtgctgat ccagcacgtg
    14161 ctcgggctgg cgacggcgat gctgctgttc ctgatcgtgc ggcggctggg cggctcgcag
    14221 tgggtggcgc tcgtgccggc cgcggcggtt gccctgtgcg gcagccaggt gatgatcgag
    14281 cacgcgctgc tctcggagcc gctgttcgct ttcctggtgg ccgcggcgct gtacgcgtgc
    14341 gtgcggtcgc tcgacgacgg gatcgggtgg gccgtggccg cgggcatgct gctcggggcg
    14401 gccgtgcccg tgcggctcgt cgcgctcggg ctgataccgg tggcggcgat ctggctcgtg
    14461 ctggcctcgc cgcggcggcg gctgctcgtg gggctggcgt tcacggtcgt ggcggcgtcg
    14521 atcccggcgg cgtggggggc ggtcaacgac ttcacgcgca ccgggcccta caacttctac
    14581 ggacgcgtcg gaccgttcgc ggactgccac cgcttcgatc ccccgagcgg cacgcggatg
    14641 ctgtgcatca agagcccgcc ggacaggcgc ggcagcacgt actcgttcat cttcacgggc
    14701 ggatcgccgg cggtcgcgta cttcggccac ccgcaggggt accccgagcc gactaaggat
    14761 gacgtcgacc ggatccgccg cttcgcgcgg gccgcggcga tcgggcagcc gctcgactac
    14821 gtgcgcaccg tcgccaagga cagcttccgc tacctggcgc cggaggtctg gcggccgccg
    14881 ccgggcgacg cgacgcagat gccggaggag ctgaaccagt acttcacgag ctcgggctgg
    14941 accggcacgg cggtgcggcg ctcgcgcgcg tactacggcg gcggggtgca cccgacggcc
    15001 aacgagccgc tgctcgaggc gctgcgcgtg tacgagcgcg ggacgcgctt cacggggctc
    15061 gcgttcgtgc tggtgctgct gccggcgctg gcggcgccgt tcctgctgcg cggccgcgcg
    15121 cgcgccggtg cgctgctgct gctgttgtgc gggctggccc tgctggccgt gccggtggcg
    15181 acgatcttct acgactaccg cttcgcaatc cccgcgctgg gcgcgctggc gaccgcgggg
    15241 gccctgggcg cgggcgagat cgcggggcgc gtgcgcgcgc gtcaggatgg cgcggcatga
    15301 ggccggtctt ctggttcgac ttcaactcgc cgttcgtgta cctggctgcg gaacggatcg
    15361 acccgctgat ccccgacgcc gaatggaggc cgttcgcgtt cccgctcatg ctcggggctc
    15421 aggggcggct cgaggaggcg atgggccgcg atccgcacgc cgcaatcgaa gccgcgtccg
    15481 gtcgtatcgc cgagcgcggg ctgccgccgc tcgcactacc ggcggggtgg ccggcagcga
    15541 cgtggtcact gcacccgctg cgcgcggccg tgtacgcgga cgagcagggg cggatgcgcg
    15601 agttcgtgcg ggtggcgtac cggaggttct gcgccgaggg gttggtcctg agcgaggtgg
    15661 cgaacctgcg cgcggcggcg gaggcggcgg ggctcgaccc ggatgcggtg ctggacgcga
    15721 tcgagcgacc cgacatcaag cagcgggtga aggacaacac cgatgcggcg atcgcccgcg
    15781 gcctgcccgg catccccacg gtcgaggtcg ggggcgagct cgtatggggc gacgaccggc
    15841 tcgacgaggc ggcggcgcta gcggagcgtg ccgacggcca ctaggtactc cttctcgaag
    15901 cacgcgtcgt cgggggtgcc gccccagagc ggggcatggc gcgacccggc ggaatgcacc
    15961 ggacgcgcca agctgagagg atcggcgccg tggccgacgt cctctcacag cttgcggttg
    16021 ccatgacacc cgccgacgcc ctggcgcgcg cgaagtgggc cgcgggccag ctcgggttga
    16081 agctgcacga cgagacggcc gacggcttcc gcggcgggag cgagtggtgg ggcgcgcacc
    16141 agatcacggt gcgcgtgacc gcgagcggct ccggcacgct ggtggctctg aacgcgtcca
    16201 cggtgaagat cggctacacg ggcaggctca agcaggtcac gcagcggttc gtggacctca
    16261 tgacggcctc cagcgagagc ctctcgatgc cggtcgaggc ggctcagccg ctcggcgacc
    16321 gcgacctgct cgtggagaag atgctcagcc gcatggaggc gagggcggag aaggaggaga
    16381 gggtggagca gctccccgcc caggcggtgc tggggttcta ccccctgaag gaccacgggc
    16441 cgctgatcgg cgctcccggc caggggaccc atagccgcac cgcgccgccg gacaactggc
    16501 agtccgacaa cgccgtggac atcaaggtcc cgaaggggac gccggtggtc gcggtcgaga
    16561 acggggtgat cggcgaccgc atcggaccgc tcaactcacg cgacccgcgg atgggaggcc
    16621 tgcgggtcta cgtcgagtcg cgcgacggga acgagttcta ttacgcgcat ctctcgaagc
    16681 tggcggtgaa ggcgggccag cgagtggaga agggccaggt catcggctac tccggctcgg
    16741 cggccggagt ggaccacctg cacttcgcgc agcgcaaggg cgacccgcgg acgacgctga
    16801 agagcgacta ggggcgcgga ccggcccgaa cgcggccggg cggcctgact ggatggggcg
    16861 ccgcaccgaa ggtgctctaa tcccacccgt gggggtcgtc cggggaatcg cggcagtcgt
    16921 ggcgctgctc gcgctcgccc tcgcgcctgc cgtcgagggt gccaagagca agccgcggcc
    16981 gcgcatcgcc gtgaacgtgc tcacgctcag ccagcgtgag atcgtcaagc agcgcgcggt
    17041 gcgggtcgag gtcacgcccg agcgcacggc caccgtgcgg ctgcgcgcgc tcgtgcggcg
    17101 caccagcaag cgctccgacg acatctcgcg cacgcgcgag ctgcgcatca aggcgggcaa
    17161 gcggcggatc gtcacgctgc ccgtctccaa gtccgcgctg gcgctgtttc ggacctgcaa
    17221 ggtcgtgaag gtcacgatct cggcgcgggc ccgcggtctc gcgccccacc gggccgcggc
    17281 tccgtcgcgg cggctgccgc cgtacgtgcg ctgcccgggc gcaccggatc ccgggccgct
    17341 tccgccgttg acctactcgt tcggcctggc ctcccgctcg atcaacccag atcccgacgg
    17401 gcgctggaag ggcgagccgg tgttcctcgg cggctatggc atcgggggcg gcacgccgtt
    17461 cctgtcgggc cgggcggcga cgggcattct ggccgagggc ccgcaggtgc gggcgttcgc
    17521 ggtcggggac ggcaagaaca cgatcgccgt cggcgacatc gaggtgcagg gctggttcgt
    17581 cgcgaacaag gacgcgccgt acgggatcgt ggaaatgcgc aaggaggtcg agcggcgcac
    17641 gggcggcgcg ctgaaggccg agaacgtgac gatccagtcg aaccattcgc acggcggcgc
    17701 ggacgcgatg ggcgtgtggg gggactcgcc gctcgactac cggcgcttca tgttcgagca
    17761 ggccgtggac gcggtggtcg aggcgttcca gaagcgcgag ccggcgaacc tctactacgg
    17821 cgccgtggac gcgcgcgacc tgctctcgaa ccagttcggg gacgacccgt tcaacgagac
    17881 gagccacaac gacacgatgg actcggacct gcgcgtgctc caggcgcgcg acgcgcgcga
    17941 caagccgatc gcgacggtcc tgaacttctc cgcccactcc acggtgctgg gatcgagcaa
    18001 cacccgaatc acgggcgact ggtcccagag ggcgaaccgg atgctcgccg agcgcttcgg
    18061 gggcaaggcg atgacgatga tcgggacgtt cggtcgcagc cagccgaacg accgcggctg
    18121 cgcgaccaag gagaaggccg accagaaggt ggaggccgac gcggtgtgcg tgctcgactc
    18181 gtatgccgag cgggtggtcg accgcgcgca ggaggcagtg cgccgggcga cgctcatcgc
    18241 cggagacccg gtggtggagg cgcggtcgta cctaatccag gacctcgcgc agaacggcgt
    18301 cgtctacctg ggtctcgggg tcgcggggga tccggtggga gtggggatca accgctcgct
    18361 cacgccgccg tgggtcaccg gcgacctgct gggcacggtc acgtcgacgc tgcggatcgg
    18421 cgacgtgatg atctcagcgg tccccggcga gatctacccg cagatcgcgc tgaaggtgcg
    18481 cgacaccgtg aagggcctgc gcgggtacat gacggccggg ctcgccaacg accagctcgg
    18541 ctacatcatc gcgccggtcg aggcctaccc ggaccccgtg cgccgcacgt tcttcaactc
    18601 gcgcggcgac gaggtcgacc cgatctcgaa cgacaactac gccttcaacg tctcgcacac
    18661 gctgggggag cgggtgatct gctcactgct gcgcggcgcc ggcgaggcgt tcggcaaggg
    18721 tgcggcgttc cgtgactcct acgagcgctg caacctgttc ccgaacgacc tcgccttcga
    18781 gcacgggacg gatacgacgt tcccgtcgcc ctgacgccgg cggctcagaa ccgccgcggg
    18841 cggtcgtcga cgaggacgcc gcggcgcggc acgtcgtcga ccacgacgcc gcggcgccgg
    18901 gccattgccg tccagagcag cgacagcacg aggccgacga tgccggcgac catgaggatc
    18961 acgcccaccg tctgaatgtc gatgccggag accgaggcgg tcacggcgta cttgaggatc
    19021 gctcccacag ctatcaggac gatcgaggta ccaatgccca tcgtggttcc ttccgtaggg
    19081 gtaccccggt agttcccgcc gcgcgccgcc acgaatcgtc cccagtcctc gagtcgacgc
    19141 gtgttcgacc tgttgcggtg cctgggcggg gtcgatcacg gctgccgccc agctccggct
    19201 gtaccccgtt cgggggaggt ctggtagaaa gccgcgccta tgactacacg cacacgcctc
    19261 tcactcatcc tggccgcgac cttcgtgctc gccgccgtgc ccgccggcga cgcctccgcg
    19321 gcgagctgct cggccaaggg ctacaagacg ctcgaccaga acaagtacat cgtcgtccag
    19381 cgcgggccgg gcccgaaggg cgactcgacg agcgactacg gcgtgtacgc ctgccggatc
    19441 agcaccggaa agcgccgcgt gctcggcggc aacgagtgct tcgggaacga cctgggcgag
    19501 gtcggcgatt acgccctggc gagcaactac atcgcctatg aggcgcgcac gtgcggcgat
    19561 ccagagggcg agtccgacgc ggtgtcgttg aacgtggcga ccggggcgaa gaagcgcttc
    19621 tcagcggagc acgagccgct ggccggcgga gtggtcgact actcgatcac gtccctgaag
    19681 gtcaacagca agggcaacat ggcctggatc gcgcaggcga agcacctctc cggcggcaac
    19741 cagacgcgct tcgacgtgcg ccgagcgatc aacggcgtgg cggaccgggt ggacgtaggc
    19801 cccggcatcg tgggcgactc gctggcgtac agcgcggggc ggatctactg gctgaacggg
    19861 gacgtgccga aggtggcggc gctgccgtag gcggagcggc gcgcgaacgt ttcgcgcgcc
    19921 cgatagcggc gacactgccg actggccgag cggcactgcg aagtacgagc ttcggccagg
    19981 aggacgccgc gagctacccc cacgactaca aggccgagcc gatagtggcc ccggtggacc
    20041 ggatggacgc ggagacgcgc acggcgcggc taagccggcg acaggttcgg agccaagtac
    20101 ttcgtccgct tgtgagcagc gccggccctt acagagcctc aacttccagc aaggcgctta
    20161 tacgactgtg caaggtacga agtaggtcga gtctcttctt ggccacgatc gtgtgatcaa
    20221 aacgctgctc cagcacgcgc tgagatacgg cgcgcttcga cagctcgatc tggctcccgt
    20281 ccacggattg cttcttgcac cagttggcga cggcacgtgc gcgtccgata tccggaatct
    20341 caggcgccgg aatatccaga ccgtgccgct cacgaagctc agcctgcacg gcagcgaggt
    20401 agatgtcgcg gctaatcaaa tcttcaagcg acaaaccctg gttcgtacga ccgagtcgaa
    20461 ggatgcgcgc agcctgtacg ccggcagtca cgaggcgagt acagtggcga tccccgtcgg
    20521 gatcgctatc gacgaggtac acaacgcgag ccgcggtgag atcaagttgc gccaacgctt
    20581 ctggcgcagc ttccgcaata cccggcgcga cctggaaccc gagcttctcc gcaccgaccg
    20641 cttgacgtaa aagagtcggc aacatgattg cgtcggtgat cccctctacg accagtgcga
    20701 accgcgcagc agtgaatgca aaggtgcccg cacccattgc aataagcagg ggtgagaacg
    20761 caccgccctg aacatcagac gaccatatgc ggttcagcac gcggctgtgt tgccattcag
    20821 cggggtccgt gtcatcgggc ccggttggag taaccagccg cactcccgcg ccgagatctt
    20881 cgggcaggca accagccgag tgagttgagt aaataacctt gccggcaacc gcttgattgg
    20941 tgagtacttt gatcagatcg gcttgcgcgt cgtaatgaag atgcgtctcc gcctcgtcga
    21001 tcagcaacac caggttgttc cgcgatgcgt gccgctcgac atatgaagcg agggcgacga
    21061 actgccggag gccgtccgac cgttcggcga tgctgatgaa tcggccaggc gcactgctta
    21121 ccaaaatatg tagctcgccg gcagttaccc gcaagcgaac cgtcaggttc gcctgactcc
    21181 aatatctttc gaacatttct gacagccgtg cattcgcggc aacctctagc tcttctacag
    21241 tcgcctcgtc gctcgcctca acagcgagcc gaaggcgctg taagtcgagc tgagccagcg
    21301 ccaagaagtt ctcaagcgca atcgtgggcg tctcgctgat gaggtgaaca ggagcgagtt
    21361 ctcggtgttc gtccgcgaag aacaggaact ccggccggcg ctcgagcaac cgtcgcgcgc
    21421 tctcttctcc aggctcaggc cggcgttcgt aatttgcaag ttgttgcagt tcgtctgcaa
    21481 gtcggattgc ggtcgggggg gcatcctcat attcacgcag atacccggcg caggtttcca
    21541 actcgtcaat agcctcgttc ggcaacgtct cttcttctgc gtcgagcgcg gaccgggcga
    21601 ggttctcgat ccggtcgaac ccctcggcag accgtgccca cttctgtccg gcgagtctgg
    21661 cgagggcgtc acgtgcatga cgtcgaggcg ctcggtcgcg ctccatctcc ggctccacgc
    21721 gcccgtaaag gatgccgctt tcacgcttcc atatctcgaa ggtgcgaatg cggcggtcgg
    21781 ggtcgacgtc ggcaacggct tcgcggtctt cggcatttag cccgaagatt gcccgcacac
    21841 agacgcggcc gtcagagccc cgggtgtagt cggccgcacc gaacgggccc gaggcgttta
    21901 aatggagcag ggctcggagg aaggaggttt tcccggcttc gtttgggcca acgattgcta
    21961 ctacccgggc acccacgtcc atgtttggct gctggtcccg aaatcggcgg tagccccaca
    22021 ggccggcaga gaggagatgc acggatgcaa cgtatccgac tgatgtggcg tttgcgcggg
    22081 atcccttgaa tactgcgcag cagcggtaaa cgcgggccaa tgcgcgcaga acgtcgcccg
    22141 cgcggcggtg ccctacaaat tcacagccac atacttcgtc tcgaggta
//
LOCUS       DASVPL010000154         6351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_19190, whole genome shotgun sequence.
ACCESSION   DASVPL010000154 DASVPL010000000
VERSION     DASVPL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19190"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF517_08005"
     CDS             <1..216
                     /locus_tag="VF517_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052921.1"
                     /translation="GNRDRGAPTPTTTATATPRAGAPAAPTAPAGGEFAPDAAASTEP
                     AERPQPQPERQAAPEHDSASGGGEFAP"
     gene            complement(314..514)
                     /locus_tag="VF517_08010"
     CDS             complement(314..514)
                     /locus_tag="VF517_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8052922.1"
                     /translation="MVLAQNMRAERRKQGMSQDALARASGIHSSEISRIERGAREPRF
                     STLVRLARALEVTPSELLERVR"
     gene            931..2310
                     /locus_tag="VF517_08015"
     CDS             931..2310
                     /locus_tag="VF517_08015"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX8052923.1"
                     /translation="MLERIDLPVDRIPSRWYNALPALPRALDSYIDNATGAKASDEYL
                     ARLMSGATVAQESSLERWVGIPEPVRDAYKMWRPTPLVRAVALERHLDTPAEIYFKYE
                     ATAPTGSHKLNSALAQAYYAKREGHTRLVTDTGAGQWGSAIALACRLMDIELLVFMVR
                     ASYDQKPYRRYLMETYGAEVRPSPSLTTEFGRATLAEAPDHPGSEATAVSEALECVLA
                     DEGSRFCVGAFTNHCLLHQTVIGLETREQLEEIDREPDYLVASFGCGSNLGGLILPWI
                     EDKQNGAEIELVAAEPEACPTLTRGDYRLDYSDASGAGPSVFSYTLGHEFVPPPIHAG
                     GLRYHGSAPLMGLLRHEGLLDARAYPQKEVFDAGVTFARLQGYLPAPETCHAIRAVID
                     IASKCREDGRPATIVFCYSGHGLLDLEAYARHGAGTLEEAGAVEVAGADQEADALAAL
                     RASRTPVLA"
     gene            2343..3905
                     /locus_tag="VF517_08020"
     CDS             2343..3905
                     /locus_tag="VF517_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX8052924.1"
                     /translation="MEGAGWNYVLHMSASLSDPDRAFDLKRALELGSEVHPTDDVVAR
                     CAWGGDRENALALEAASAAAAVYGATPLETRKAILRDFHVAIRDRADELVEVLVAEGH
                     PRRLAEWELSGLLRCCDRETLDWYAQQFESRYECDGRQLTQVRRPDGVVCINPPQNAG
                     TASSGFASLALLAGNAVVVKAPRSCPYGVMFMCRELIAPILEAHGAPPGTLNLISGDT
                     RRILRQWMTSPHVDDVFFVGDSETGLRLGAECVARGKKPILELAGNDGFLVWRDADIE
                     AAASALTECFLGSGQICMIPKYAIVHPAIADEFVDAFLERVAEIRPGYPDDEDVVLSP
                     VLKVDRFFDFLAEARDAGCEVLAGGRRVDVDGDPSVTGAFVEPTVVRVDGMRDARRLS
                     CVREETFFPLIPIVVPQASEEPGLLDAAIRFVNENEYGLRNSVWASDDYVLGELARRI
                     VNGGLLKINDSHAAFTSYLATHGGTGKSGGPFGELNYFPLRTSHLQGICWGTGRPDLI
                     ASPFAAAVAAPA"
     gene            complement(3917..4393)
                     /locus_tag="VF517_08025"
     CDS             complement(3917..4393)
                     /locus_tag="VF517_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8052925.1"
                     /translation="MTRMMDAFGDRWTFLILRQAFFGVRRFNQMQRQLGVSRNILAAR
                     LRMLVDTGVLKRRRYRRDPDFYEYRLTERGLALYPAILAFSRWGDRYLPRDESSGTLV
                     LRHKPCGEVADPVLVCSCCGEPIHARDVEVYVADHGPPLEDGELQAPDLRPRSAEA"
     gene            complement(4724..5068)
                     /locus_tag="VF517_08030"
     CDS             complement(4724..5068)
                     /locus_tag="VF517_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052926.1"
                     /translation="MTVTAAVAAGCGGEKTDVSGGVDNLNERLTQQGIPAKLECPKEV
                     DGGEGTGFECELKANQGGKTQKIQMEVQKEGDDLVVDVKDQQAFEKALAAVAGGQQQQ
                     QGGGQQAPPEGE"
     gene            5181..>6351
                     /locus_tag="VF517_08035"
     CDS             5181..>6351
                     /locus_tag="VF517_08035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8052927.1"
                     /translation="MAATRVTRTLVALVGLVVAAAALRFATLDLQSFWVDEGATVNLL
                     RGDFGHLLDGIPVTEKTPPLYYVLAWLWTRPFGTGEIGVRSLSALAGVLTVPAAFALG
                     RELVSERAGLVAAALVAFNPMLVWYSQEARAYALLVLLSALATLYGVRAAREREGPLW
                     RWAAVSALALATHYFALFVIAPLALWILARRVDRRRALVATAAIAAAGVALLPLAIEQ
                     SENPGSNFIGGTALATRIAQVPKQFLLGFDAPLEAAFTIAALALAAAGAWLALRPRGR
                     AEPPGGAAAGAELPGGAAAGAEPPGGPASGAAPPGEPASGAAPPGGARIALALAAAAI
                     ALPVAAALAGADFVLARNLIVALVPFAVLLAAGFASASRLGLALAAALAALSLATV"
BASE COUNT          867 a         2329 c         2219 g          936 t
ORIGIN      
        1 ggcaaccgcg accggggcgc gccgacgccg acgacgacgg cgacggcaac gcctcgggcc
       61 ggtgccccgg ccgcaccgac ggcgccggcg ggcggcgagt tcgcgccgga cgcggccgcc
      121 tcgaccgagc cggcggagcg accgcagccg cagccggagc ggcaggccgc gccggaacac
      181 gacagcgcct ccggcggcgg cgagttcgcg ccgtagggat agaccgcgac ggaccccgag
      241 agcgcgtccc agaggcggcg cgttcgcgcc gtagaacgcg agcgcccgga cgctcgagga
      301 acggcggcag cgactagcga acgcgctcga gcagctcgct cggcgtgacc tcgagcgccc
      361 gcgcgaggcg cacgagcgtc gagaagcgcg gctcccgagc gccgcgctcg atacggctga
      421 tctcgctcga gtggatccca ctcgcgcggg cgagcgcgtc ctggctcatg ccctgcttgc
      481 gccgctccgc gcgcatgttc tgcgcgagca ccacgagcgg ctcactcccg gccggatcga
      541 cgtcgtaccg gccggcggct cgtgtcgatc tgtgtgagtt cccctcttgt cccaacttgc
      601 gtgctttcga tcggcgactg cgacttgttg gtagcaaatc tataccggtc gcgaatctgg
      661 accccccatg cggtgctggc gcagcgtaaa ccgacccccc cgacaggggg gggtctgtca
      721 cccctttgat ttcgcccgaa aatgcgtaca gggcgtacgg cgtcgaccgg ccgagccatt
      781 gcgcgcaccg gaggacgggg tttacgctcg cttccgcgat tcggtgctca atcgcgacca
      841 gtcgcgagac tggaccacgc aattcgcagg gcgggcgcgt cgcgtctcgt cttggtcatc
      901 acggaggagc cagaactgcg cacgaaaggc ttgctagagc ggatcgacct gccggtcgat
      961 cgaatcccga gccggtggta caacgcgctt cccgcgctgc cgcgcgctct cgactcgtac
     1021 atcgacaacg cgaccggcgc gaaggcttcg gacgagtacc tcgcacgcct gatgagcggc
     1081 gcgacggtcg cgcaggagag ctcgctcgag cgttgggtcg ggatccccga accggtccgg
     1141 gacgcctaca agatgtggcg tcccacgccg ctcgtccggg ccgtcgccct cgagcggcat
     1201 ctggacaccc ccgcggagat ctacttcaag tacgaggcga cggcgccgac ggggagccac
     1261 aagctcaact ccgcgctcgc ccaggcgtac tacgccaagc gcgaaggaca cacccggctg
     1321 gtcacggaca cgggcgccgg gcagtgggga agcgccatcg cgctcgcctg ccgcctgatg
     1381 gacatcgagc tcctcgtctt catggtcagg gccagctacg accagaagcc ctaccgccgg
     1441 tacctcatgg agacctacgg cgccgaggtc cggccgagcc cgagcctcac gacggagttc
     1501 ggacgggcga cgctcgcgga ggcccccgac cacccgggca gcgaagccac agcggtcagc
     1561 gaggcgctcg agtgcgtgct ggccgacgag gggtcacgct tctgcgtcgg cgccttcacg
     1621 aaccactgcc tgctccacca gaccgtgatc ggcctcgaga cgcgcgagca gctcgaggag
     1681 atcgaccgcg agccggatta cctcgtggcc tcgttcggat gcgggagcaa cctcggcggg
     1741 ctgatcctgc cgtggatcga ggacaagcag aacggcgccg agatcgagct cgtcgccgcc
     1801 gagcccgagg cgtgcccgac gctcacgcgc ggcgactacc gcctggacta ctcggatgcg
     1861 tcgggcgccg ggccgagcgt cttctcgtac acgctcggcc acgagttcgt cccgccgccg
     1921 attcacgccg gcggcctccg ctaccacggc tcggccccgc tgatgggcct gctccggcac
     1981 gagggactgc tcgacgcgcg cgcctacccg cagaaggagg tcttcgacgc gggggtcacg
     2041 ttcgcgcggc tccagggcta cctgcccgcg ccggagacgt gccatgcgat ccgtgccgtg
     2101 atcgacatcg cgagcaagtg ccgggaggac ggccggccgg ccacgatcgt cttctgttac
     2161 agcgggcacg gcctgcttga cctcgaggcc tacgcacgcc acggagccgg caccctcgag
     2221 gaagcgggtg ccgtcgaggt ggcaggcgcc gaccaggaag cggacgctct cgccgccctg
     2281 cgcgcgagtc gcacgccggt cctcgcatga ggtcgtaccc cctttacatc ggggggcgcg
     2341 acgtggaggg ggctggctgg aactacgtgc tccacatgtc cgcctccctc agcgacccgg
     2401 accgggcgtt cgacctgaag cgggccctcg agctgggcag cgaggtccat cccaccgacg
     2461 acgtcgtggc gcgctgcgca tgggggggag atcgcgagaa cgcgctcgcc ctggaggcgg
     2521 cgagcgcggc ggccgccgtc tacggtgcca cgcccctcga gacacggaag gcgatcctgc
     2581 gcgacttcca cgtcgccatc cgcgatcggg ccgacgagct ggtcgaggtc ctagtcgcgg
     2641 agggccatcc gcgccggctc gcggagtggg agctgagcgg cctgcttcgc tgctgcgatc
     2701 gcgagacgct cgactggtac gcgcagcaat tcgagagccg ctacgagtgc gacgggcggc
     2761 agctcacgca ggtgcgcagg cccgacggcg tcgtatgcat caacccgcct caaaacgccg
     2821 gcaccgcgag ctcggggttt gcgagtctgg ccctgctcgc cgggaacgcg gtcgtggtca
     2881 aggccccgcg gtcctgcccg tacggcgtga tgttcatgtg ccgcgagctg atcgccccga
     2941 tcctcgaggc tcatggcgct ccgccaggca cgctgaactt gatcagcggc gacacccgcc
     3001 gcatcctccg ccagtggatg acgagcccac acgtcgacga cgtcttcttc gtcggcgaca
     3061 gtgagaccgg gctgcgtctc ggcgccgagt gcgtggcgag aggcaagaag ccgatcctcg
     3121 agctggccgg gaacgacggg ttcctcgtgt ggcgcgatgc cgacatcgag gcggccgcga
     3181 gcgcgctgac cgagtgcttc ctggggtcgg gccagatctg catgatcccc aagtacgcca
     3241 tcgtgcaccc cgccatcgcg gacgagttcg tcgacgcgtt cctcgagcgc gtcgcggaga
     3301 tccggcccgg ctacccggac gacgaggacg tcgtgctgag cccggtgctg aaggtggacc
     3361 ggttcttcga ctttctcgcg gaggcgcgcg acgcgggctg cgaggtcctc gccggtggac
     3421 ggcgcgtgga cgtcgacggc gatccctcgg tcaccggcgc gttcgtcgag ccgacggtgg
     3481 tccgcgtcga cggcatgcgc gacgcgcggc gcctctcctg cgtccgcgag gagacgttct
     3541 tcccgttgat cccgatcgtc gtgccgcagg cgtcggagga gcccggcctc ctcgatgccg
     3601 ccatccgctt cgtcaacgag aacgagtacg ggctccggaa ctccgtctgg gcttccgacg
     3661 actacgtgct cggcgagctc gcgaggcgga tcgtcaacgg cgggctcctc aagatcaacg
     3721 actcacacgc cgcgttcacc tcgtacctgg cgacccacgg tggaaccggc aagagcggcg
     3781 gcccgttcgg cgagctcaac tacttcccgc tccgcacgtc ccacctccag gggatctgct
     3841 ggggcacggg tcggcccgac ctgatcgcct ctccgttcgc ggccgcggtc gccgcgcccg
     3901 cctgagctcc cggcgcttac gcctcggccg accgcggccg caggtccggc gcctgcagct
     3961 cgccgtcctc gagcggcggg ccgtgatcgg cgacgtagac ctcgacgtcg cgcgcgtgga
     4021 tcggctcgcc gcagcaggag cacaccagca ccgggtcggc cacctccccg catggcttgt
     4081 ggcgcaggac gagggttccc gaggactcgt cgcgaggcag atagcggtcg ccccaacgcg
     4141 agaaggcaag gatcgccggg tacagcgcga gaccgcgctc ggtcagccgg tactcgtaga
     4201 agtcggggtc ccggcggtag cgacggcgct tcagcactcc cgtgtccacg agcatccgca
     4261 accgggccgc gaggatgttc cgcgagacgc cgagctgccg ttgcatctgg ttgaacctgc
     4321 ggacgccgaa aaacgcctgg cgcaggatca ggaacgtcca gcggtcgccg aacgcgtcca
     4381 tcatccgcgt catcggatcg tcgacccgca gggccggctt cgagcgaacc gagtcgatcc
     4441 gcccgggcgg aatcagcgcg ccctcccccg tgcgctttgc ccgcgcgcgt ggcgcttcgc
     4501 gctcatcggc gtggcggtga ctcccgttgt caccttctga cggcatctcc cgcgcgggaa
     4561 ctctacgtgc tggagcaagc gctgtgtgac gcaagtccgc tgaaaggccc gattggagcg
     4621 gccctctacc ccccgatccg agggggtatc ccccctacat gggggatgag cagcgccacc
     4681 cgccgggaag accccccggc gggggcgagc tcgcgcctcg ggcctactcc ccctcgggag
     4741 gggcctgctg gccgccgccc tgctgctgct gctggccgcc ggccaccgcc gccagcgcct
     4801 tctcgaacgc ctgctggtcc ttgacgtcga ccaccaggtc gtcgccctcc ttctggacct
     4861 ccatctggat cttctgcgtc ttgccgccct ggtttgcctt cagctcacac tcgaagccgg
     4921 tgccctcacc gccgtcgacc tccttggggc actcgagctt cgcgggaatg ccctgctggg
     4981 tcagccgctc gttgaggttg tcgacgccac cgctgacgtc cgtcttctcg ccgccgcagc
     5041 ccgcggctac ggctgcggtg acggtcacga gcgccagcgc ggcaccaatt cgacgtagcc
     5101 gatccatatg ggtagaacct ttcttcgaga tcaaggagag acgggcaggg taatcgcact
     5161 gcctcggata gcctcgccac gtggcggcca cgcgcgtgac caggacgctc gtcgcactcg
     5221 tcggcctcgt ggtcgcagcg gccgcgctga ggttcgcgac gctcgatctc cagagcttct
     5281 gggtggacga gggcgccacg gtcaacctcc tccgcggcga tttcgggcac ctgctcgacg
     5341 ggatcccggt caccgagaag accccgccgc tctactacgt cctcgcctgg ctctggacgc
     5401 gtccgttcgg cacgggcgag atcggcgtgc ggtcgctctc ggccctcgcg ggagtgctca
     5461 ccgtgccggc cgcgttcgcg ctcgggcgcg agctggtgtc cgagagggcc gggctcgtgg
     5521 cggccgcgct cgtcgccttc aacccgatgc tcgtctggta ctcgcaggag gcgcgggcct
     5581 acgccctgct cgtgctgctc tcggcgctcg cgaccctcta cggcgttcgc gccgcgcgcg
     5641 agcgcgaggg gccgctgtgg cggtgggccg ccgtctcggc tctcgctctc gccacgcact
     5701 acttcgccct cttcgtgatc gcgccgctgg cgctgtggat cctcgcgcgg cgcgtcgacc
     5761 ggcgccgcgc gctggtcgcg accgccgcga tcgcggcggc cggagtggcg ctgcttccgc
     5821 tcgcaatcga gcagtccgag aaccccggct cgaacttcat cggtgggacc gcgctggcga
     5881 cgcgcatcgc gcaggtgccg aagcagttcc tgctcgggtt cgacgccccg ctcgaggccg
     5941 cgttcacgat cgccgccctc gcgctggccg cggccggcgc gtggttggcg ctgcggccgc
     6001 ggggccgagc tgagccgccg ggaggggcgg cggccggtgc ggagctgccg ggaggggcgg
     6061 cggccggtgc ggagccgccg gggggacccg cctccggcgc ggccccgccg ggggagcccg
     6121 cgtccggcgc ggccccgccg ggcggcgccc gcatcgccct cgcgctggcc gccgccgcga
     6181 tcgccctgcc ggtcgcggcc gcgctcgccg gcgccgactt cgtcctcgcg cgcaacctga
     6241 tcgtggcgct cgtcccgttc gcggtcctgc tcgcggccgg cttcgcgtcg gcgtcgcgcc
     6301 tcgggctcgc cctcgccgcc gcgctcgcgg cgctgtcgct cgcgaccgtg c
//
LOCUS       DASVPL010000155         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_293319, whole genome shotgun sequence.
ACCESSION   DASVPL010000155 DASVPL010000000
VERSION     DASVPL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293319"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="VF517_08040"
     CDS             <1..1648
                     /locus_tag="VF517_08040"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8052928.1"
                     /translation="WAADQGAAFSEAVRAAAYLGLFVLVVMAAPAGGARGWLAGLALG
                     LLAVSVLALGSRVEPSLFPEQRLADFLPGLTRLSYPLNYWNALGACMALALVLLAWFG
                     GHAESRAGRALAVAATPFPALVLYLTSSRGAVVALAAGLAALLALGPGRARMLGGLLL
                     GGAGSALLIVLAESREAFVDGRVEAAGAAAEGREMLLAAVLVGAVLGAARLAFDRPLD
                     GLVVPRAVARAGAGAVAVALMVGIVASDPAERLDRFNDPPVTEVTTRGFVADHLTSSA
                     GAGRYQFWGSALDAFESEPVRGIGAGGYEAWWARHGSLRYFIRDAHSLFAETLAELGL
                     LGLAALLVFLGGAVVAGLTARRAPPRAAPAAAALAVLTCATVSSAVDWTWEVPAAFAP
                     LVVAAALLAGPAAAAGERGGESRFGLGVATLGLAWVAAIAATVALATEVKLDDSREAV
                     RRGDLGQAASDAAAARALSPWSGVPRLQLALVRERRGDLGGARRAAGEAADRDGQDWR
                     VWLVVARLALHDGDVPGARRALRRARTLNPRSPIFRAPGP"
     gene            1663..2718
                     /locus_tag="VF517_08045"
     CDS             1663..2718
                     /locus_tag="VF517_08045"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8052929.1"
                     /translation="MHAGAPTTEPDTRRPRALVTGSAGFIGSHVCDACLELGMDVVAT
                     DDLSGGFRENVPEAASWVEGDVRDPSFCASLWEGGRFDYVYHLAAYAAEGLSHFVRRF
                     NYETNLVASVNLINLAIEHKVERFVFASSIAVYGAGQTPMTESLEPRPEDPYGVSKYA
                     VELDLRAAHDVFGLDYTVFRPHNVYGERQNVADRYRNVIGIFMNAVLRGEPLPVFGDG
                     SQTRAFSHIDDVAPLIARSPLVEGARNEVFNVGADTPYSILELAAEIGRAFETEVEIQ
                     HMPPRNEVVHAFSDHSKVREVFQPPAPIALDEGIRRMAAWVRSNGAQPPTDFPGEIEL
                     VEKLPPSWAPSLSDRAG"
     gene            2685..3992
                     /locus_tag="VF517_08050"
     CDS             2685..3992
                     /locus_tag="VF517_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HEX8052930.1"
                     /translation="MGAEPVGPRGLSVRRRLDLPALANPLIAFTALWFAGVALSQIHV
                     FSFQRNWSAFVWAIMLLAPAGFVIGGLIGRDLVARRVTPSREATELGPAQRRRLRILL
                     VLCLAVGYAEMVHQFAAAGAVPLLAENIDATRAAQPGGPTVVLTQFLTVAMILGLLAP
                     PRLLSWRAAPEIAAAGVASLGFLLAGGRGTIAIGLLGAALARMLYWGAPRPRVVLGGL
                     ALGYAALATMFFVRAGQHRGNAFEAELFDDVIPSTPVVLRPYLALHVGLTTNFNVLDR
                     LTGVFPSVHAWGYGAYSTSGFDAVVPGTEQLSDVAKEVASPFITSTAAGPYFADGGAP
                     GVVVGMALFGLVTVAAYTWARRSGSFVACMIGGYVTYIALFGVYTNLFTEHPDWAVVI
                     PCLAVAGYLVRLPEDHGPRRVAAALIDALRPPGSSGRAPGARP"
     gene            3989..4948
                     /locus_tag="VF517_08055"
     CDS             3989..4948
                     /locus_tag="VF517_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052931.1"
                     /translation="MRRLAPRRALLAVVVLLALAGVAIVAEALRDEERPRAPELRAGR
                     PIAVPARALGSARSLIVTDGDLPQDDAPVWVFDRPAGGKRAVWRLAVLGDRVEVAERT
                     SVRADADEPEGTRYAVGRWTAGADAAFRLVPRKGGVDVRVVTLDGKARQLSRGTAPVA
                     RVPGADRELAVATWQPGKLPALFVIDHGQARERVSVAIFSGESAFRTRIATFKLPVRG
                     LPARDWSLSIGRGIGNRPNLVAVARAGSSRHPEVHVVSGDSNFQTFVAQYVIGLPPLG
                     RDAAVAPGATLGLPAVYLLDRDRNDLRIVAYATTPLAAVQPPR"
BASE COUNT          515 a         1886 c         1880 g          725 t
ORIGIN      
        1 gtgggccgcg gaccaaggcg cggccttctc cgaggcggtg cgcgccgcgg cgtacctggg
       61 cctcttcgtc ctcgtggtga tggccgcgcc ggccggcggc gcgcgggggt ggctcgccgg
      121 gctcgcactc ggcctgctcg ccgtgagcgt gctcgcgctc gggagccgcg tcgagccctc
      181 gctcttccct gagcagcggc tcgccgattt cctgcccggc ctcacgcgtc tgagctaccc
      241 gctcaactac tggaacgccc tgggcgcgtg catggcgctc gcgctcgtcc tgctcgcttg
      301 gttcggcggc catgcggaga gccgcgcggg gcgtgccctg gcggtcgcgg ccaccccgtt
      361 cccggcactc gtgctctacc tcacgtcctc gcgcggcgcc gtggtggcgc tggccgcggg
      421 gctcgccgcc ctgctcgccc tggggccggg acgggcccgg atgctcggcg gcctgctgct
      481 cgggggtgcc ggcagcgcgc tgctgatcgt cctggcggag tcgcgcgagg cgttcgtgga
      541 cggccgggtc gaggccgccg gcgcggccgc cgaggggcgc gagatgctcc tcgccgccgt
      601 gctcgtgggt gcggtgctcg gggccgcgcg gctcgcgttc gaccgcccgc tcgacgggtt
      661 ggtcgtcccg cgcgcggtcg cgcgcgcggg cgcgggcgcc gtcgcggtcg cgctgatggt
      721 cggcatcgtc gcctccgacc cggccgagcg gctcgacagg ttcaacgacc cgccggtgac
      781 ggaggtcacg accaggggct tcgtggccga ccacctcacg agctcggcgg gcgccgggcg
      841 ctaccagttc tgggggagcg ccctcgacgc gttcgagtcc gagccggtcc gcggcatcgg
      901 agcgggcggg tacgaggcct ggtgggcgcg gcacgggtcg ctgcgctact tcatccggga
      961 cgcgcactcg ctcttcgccg agacgctcgc cgagctcggc ctgctcggcc tcgcggcgct
     1021 gctcgtcttc ctcggcgggg ccgtcgtggc cgggctgacc gcgcgccgtg cgcctccgcg
     1081 cgccgcgccg gccgccgccg cgctggccgt cctcacctgc gccaccgtct cgtcggcggt
     1141 ggactggacg tgggaggtcc cggccgcgtt cgccccgctc gtggtcgcgg cagcgctgct
     1201 ggcgggcccg gccgccgccg ccggcgagcg cggcggcgaa tcccgcttcg ggctcggcgt
     1261 ggccacgctg gggctcgcgt gggtggccgc tatcgccgcc accgtggcgc tggccaccga
     1321 ggtcaagctc gacgacagcc gtgaggccgt ccggcgcggc gatctcggcc aggcggcgtc
     1381 cgacgccgcc gccgcgcgcg ccctctcgcc ctggtccggg gtgccgcgcc tccagctcgc
     1441 gctcgtgcgc gagcggcgcg gggacctcgg cggtgcgcgc cgcgcggccg gcgaggcggc
     1501 cgaccgcgac gggcaggact ggcgggtctg gctggtggtc gcccggctcg ccctgcacga
     1561 cggagacgtg cccggcgccc gccgcgccct gcgccgcgcc cgcacgctca acccgcgctc
     1621 gccgatcttc agggcgccgg gtccctaatc tgccgttgtc cgatgcacgc gggagcgccg
     1681 acaaccgagc ccgacacacg gcgcccgcgg gcgctcgtga ccggctccgc cggcttcatc
     1741 gggtcgcacg tctgcgacgc ctgcctcgag ctggggatgg acgtggtcgc cacggacgac
     1801 ctctcggggg gctttcgcga gaacgtgccg gaggccgcga gctgggtcga gggcgacgtg
     1861 agggacccca gcttctgcgc gtccctgtgg gagggcggcc ggttcgacta cgtctaccac
     1921 ctcgccgcgt acgcggccga ggggctctcc cacttcgtgc gccgcttcaa ctacgagacg
     1981 aacctcgtcg ccagcgtcaa cctgatcaac ctggccatcg agcacaaggt cgagcggttc
     2041 gtgttcgcct cgtcgatcgc cgtctacggg gcggggcaga cgcccatgac cgagagcctc
     2101 gagccgcggc ccgaggaccc ctacggggtg tcgaagtacg ccgtcgagct cgacctccgg
     2161 gcggcgcacg acgtgttcgg gctcgactac acggtcttcc ggccccacaa cgtgtacggg
     2221 gagcggcaga acgtcgccga ccgctaccgc aacgtgatcg ggatcttcat gaacgcggtc
     2281 ctgcgcgggg agccgctgcc ggtcttcggc gacgggagcc agacgcgggc cttctcgcac
     2341 atagacgacg tggctccgct gatcgcgcgc agcccgctgg tcgagggggc gcggaacgag
     2401 gtgttcaacg tcggcgccga cacgccgtac tcgatactcg agctcgcggc ggagatcggg
     2461 cgggcattcg agaccgaggt cgagatccag cacatgcccc cgcgcaacga ggtcgtgcac
     2521 gccttctcgg accactcgaa ggtgcgcgag gtcttccagc cgccggcccc gatcgcgctg
     2581 gacgagggga tccgccgcat ggccgcatgg gtgcgcagca acggcgcgca gccgccgacc
     2641 gacttccccg gcgagatcga gctggtggag aagctcccgc cctcgtgggc gccgagcctg
     2701 tcggaccgcg cgggctgagc gtgcgacgcc ggctcgacct ccccgccctc gccaatcccc
     2761 tgatcgcctt cacggcgctg tggttcgcgg gggtcgcgct gtcccagatc cacgtcttct
     2821 cgttccagcg caactggtcc gccttcgtct gggcgatcat gctcctcgcg ccggcggggt
     2881 tcgtgatcgg cggactgatc ggccgcgatc tggtcgcgcg ccgcgtcacg ccgtcccgcg
     2941 aggcgacgga gctcgggccc gcccagcggc ggcggctgcg catcctgctc gtcctctgcc
     3001 tcgcggtcgg ctacgcggag atggtgcacc agttcgcggc ggcgggcgcg gtgccgctcc
     3061 tcgcggagaa catcgacgcc acgcgcgccg cccagccggg cggtcccacc gtggtgctga
     3121 cccagttcct gaccgtcgcg atgatcctcg gcctgctcgc gccgccccgg ctcctctcgt
     3181 ggcgcgccgc cccggagatc gccgcggcgg gcgtcgccag cctcgggttt ctgctcgccg
     3241 gcggccgggg cacgatcgcg atcggcctgc tgggcgccgc gctcgcccgc atgctctact
     3301 ggggcgcccc gcgcccgcgg gtggtcctcg gcggcctcgc gctcggctac gcggcgctcg
     3361 ccacgatgtt cttcgtccgg gccgggcagc accggggcaa cgccttcgag gcggagctgt
     3421 tcgacgacgt gatcccctcc acgcccgtcg tgttgcggcc gtacctggcg ctgcacgtgg
     3481 ggctcacgac gaacttcaac gtgctcgacc gcctcaccgg cgtgttcccc tcggtccacg
     3541 cctggggcta cggcgcgtac agcacgtccg gcttcgacgc cgtcgtgccc ggtaccgagc
     3601 agctgagtga cgtcgcgaag gaggtcgcct cgccgttcat aaccagcacc gccgccgggc
     3661 cgtacttcgc ggacggcggt gcgcccggcg tcgtcgtcgg gatggcgctg ttcgggctcg
     3721 tcacggtggc cgcgtacacc tgggcgcggc gcagcgggtc gttcgtcgcg tgcatgatcg
     3781 gcggctacgt gacctacatc gcgctcttcg gcgtctacac gaacctgttc accgagcacc
     3841 cggactgggc cgtggtgatc ccgtgcctgg cagtcgccgg ctacctcgtg cgcctgcccg
     3901 aggaccacgg gccgcgccgc gtggccgcgg cgctgatcga cgcgctccgg cccccgggat
     3961 cgtccgggcg ggccccgggg gcccggccat gaggcgcctg gcgccgcgcc gcgcgctcct
     4021 cgcggtggtg gtgctcctcg cgctcgccgg agtggcgatc gtggcggagg cgctgcgcga
     4081 cgaggagcgg ccgcgcgctc ccgagctcag ggccggtcgc ccgatcgcgg taccggcgcg
     4141 ggcgctcggc tcggcccgct cgctgatcgt caccgacggc gacctgccgc aggacgacgc
     4201 tccggtatgg gtgttcgatc gcccggcggg ggggaagcgc gcggtctggc ggctcgccgt
     4261 cctgggcgat cgcgtggagg tggccgagag gacgtcggtg cgcgccgatg ccgacgagcc
     4321 ggaggggacc cggtacgcgg tggggcgctg gaccgccggc gcggacgccg cgttccggct
     4381 cgtgccgcga aagggtggag tggacgtccg ggtggtcacg ctcgacggca aggcacggca
     4441 gctcagccgc ggcaccgcgc ccgtcgcgcg cgtgccgggt gccgatcgcg agctggctgt
     4501 ggccacctgg cagcccggca agctcccggc gctgttcgtg atcgaccacg ggcaggcgcg
     4561 cgagcgcgta tcggtggcga tcttctccgg cgagtcggcg tttcgcaccc gcatcgccac
     4621 gttcaagctg ccggtccggg ggcttccggc gcgcgactgg agcctctcga tcggccgcgg
     4681 gatcgggaac cggccgaacc tcgtcgccgt cgcacgcgcc ggctcgtcgc gccaccccga
     4741 ggtgcacgtg gtatcgggcg actcgaactt ccagacgttc gtggcgcagt acgtgatcgg
     4801 cctcccgccc ctgggccgcg acgcggccgt cgcgccgggc gccacgctcg gactgccggc
     4861 ggtgtatctg ctcgaccgcg accgcaacga cctcaggatc gtcgcgtacg cgacgacccc
     4921 gctcgcggcg gtccagccgc cgcgctgagc gacgtcaggc ggcgatgcgg cggaccggcc
     4981 ggcccgccgc cagcgcgcgc gcctgg
//
LOCUS       DASVPL010000156         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_214117, whole genome shotgun sequence.
ACCESSION   DASVPL010000156 DASVPL010000000
VERSION     DASVPL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214117"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..825)
                     /locus_tag="VF517_08060"
     CDS             complement(19..825)
                     /locus_tag="VF517_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052932.1"
                     /translation="MSGRRSLPGRIADRIALRRGALHERRLAEDHLRRAGRPELDAWF
                     VERHGSSVLHGPLAGLSYPPAVLADVHHLTAKLLGAYEEELSAVIAAEVERRPPLFVD
                     IGSADGYYAAGFALASPGTVVHAFEIDPVARGVLDRLVRTNGLAARVFMHGPANARRL
                     AEHDLAGAFVLCDIEGAELDVLAGEAVPALAHATLLVEVHPLAGGADTAPALRERFGP
                     THRIEAIEPRERDPAAQPELDGAPHRDSALDEYRFGRTSWLAMHPRTLPH"
     gene            914..1909
                     /locus_tag="VF517_08065"
     CDS             914..1909
                     /locus_tag="VF517_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX8052933.1"
                     /translation="MPNLPKPRLRSRSRTRVDAHAPQTAAEPNVEVVEHGGLRWINIE
                     RPGPLDRAWVEEHFEFHPLDYEDIASRNQRPKIDEYDDYLFIVLHFPVFDKSVGRLNA
                     GELDIFIGPGYLITLPNVPLQPVEYLFERCRASEETREQFFSKGAGYLLYKIVDDAFD
                     YCFPMLRKVGNKLDRVEEDIFEGRSEEVVRDISNVKQEIINFRKIIRPQRPVLQDLER
                     TKQRFLSEEHELYFDDVVDASERIWDMLENYKEVVEALEDTNESVLSHRVNDVLRVLT
                     AFSVVILPLTLIASLWGMNVGVPGEGSKAAFWIIVVAMVGMLVSMLSYFRHRGWL"
     gene            complement(1882..2982)
                     /locus_tag="VF517_08070"
     CDS             complement(1882..2982)
                     /locus_tag="VF517_08070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8052934.1"
                     /translation="MSEPAERLRIALLAPAFFPEVRRGGERLVRELADDLIARGHQPS
                     LITAHRGAPSRAVEDGLPILRLPRPPVESRLRRRAFEDYLSHAPLSYLALKHGTFDVA
                     HATHPPDAVAGARSGLPSVFTFLGLPTRTGIAQRRMRAELLVRATRECDATVAISRTA
                     ADGFRRALGVEARVIHPSVDLDAFRPTAERAPEPTIVCLADAGAENKRLPLLIDAFRQ
                     VRRERPDARLLLQRHESLRPGDGIELIDPQDRAELAQVYSSAWVSALPSWGEAFGLVL
                     VEALACGTPVVGSNLGAIPEVVDRDTIGRLFSGDEPDALARALLEALDLAADPATREA
                     CRARAADFSTERMGAAYEDLYGELQSQPRWRK"
     gene            complement(2979..3941)
                     /locus_tag="VF517_08075"
     CDS             complement(2979..3941)
                     /locus_tag="VF517_08075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8052935.1"
                     /translation="MADQPDIAVVVPSHDRPLRLRWLLNALEEQTLPRERFEVIVAHD
                     SRGPETAELLRTHPLGVRAIGFEPGPGPAAKRNAGWHAATAPLVAFTDDDCRPPPEWL
                     ERALAAARAHPGAIVQGATIPDPDEEHLVRAAPHVDTQRIVPPTPWAQTSNIVYPREL
                     LERVGGFDETFPLAAGEDTDLAIRATEAGAGYVGARDVVTFHAVHAVSLPQLVRARWR
                     WQHLAYLVKRQPSVREHFPMWIFWKRTHVWLPLAIAGALLRRRKPGAELLALPWVIHT
                     LPDRGAGPRGRARALSELPARAVVDSAEMAALARGSIRWRTFFL"
     gene            3967..4641
                     /locus_tag="VF517_08080"
     CDS             3967..4641
                     /locus_tag="VF517_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8052936.1"
                     /translation="MPAWYHTLELGPGHVVEGMFDMRPYVDRFGLPERMDGMRALDVG
                     TFDGFWAFEMERRGAEVVALDIDDEADLDYPPRRRPASFGPRGENFRLAREALGSSVE
                     RVGCSIYDARPEELGEFDLVLCGLVLVHLRDQLLALERIAGLCRGTFISGEAYDPLMD
                     LLPFAASRYKADRDADVVFWEPGSRTWRRMIWSAGFDRVERCGRFRLPSTQGFSVRCV
                     VHHARP"
BASE COUNT          655 a         1670 c         1769 g          634 t
ORIGIN      
        1 agattctaga atttcgttct agtggggaag ggtgcgcggg tgcatcgcga gccagctcgt
       61 gcggccgaag cggtactcgt cgagcgccga gtcgcgatgc ggggcgccgt cgagctcggg
      121 ttgggcggcg gggtcgcgct cccgcggctc gatcgcctcg atccggtggg tcgggccgaa
      181 gcgctcgcgc agggcgggcg ccgtgtccgc gcccccggcc agcgggtgga cctcgacgag
      241 cagcgtcgcg tgcgcgagcg cgggcaccgc ctcgccggcg agcacgtcca gctcggctcc
      301 ctcgatgtcg cacagcacga acgcaccggc caggtcgtgc tccgccaggc gccgcgcgtt
      361 cgccgggccg tgcatgaaca cccgcgcggc cagcccgttc gtgcggacga gccggtcgag
      421 cacgccgcgc gcgacggggt cgatctcgaa cgcgtgaacc acggtcccgg ggctcgcgag
      481 cgcgaagccc gcggcgtagt agccgtccgc cgatccgatg tcgacgaaca gcggcggccg
      541 gcgctcgacc tccgcggcga tcaccgcgga cagctcctcc tcgtaggccc cgagcagctt
      601 cgcggtcagg tggtggacgt cggcgagcac ggcgggcgga tacgagagac ccgccagcgg
      661 gccgtgcagg accgacgagc cgtggcgctc gacgaaccac gcgtcgagct ccggccgccc
      721 ggcgcgcctc aggtgatcct cggccagccg ccgctcgtgc agcgcgccgc gccgcagggc
      781 gatgcggtcg gcgatccgtc ccggaaggct gcgacgcccg ctcacgcgcg gcaacgtagc
      841 gcccaggtct ccgggcgccc ccgtagaatc aaccccgtgg cgcagctaac cgacgacccg
      901 ctcagtccga gccatgccga atctccccaa gccacgcctg aggagccgct cgcggacgcg
      961 ggtcgacgcg cacgcgccgc agaccgccgc tgagccgaac gtagaggtcg tcgagcacgg
     1021 cggcctgcgc tggatcaaca tcgagcgtcc cggcccgctc gaccgcgcct gggtcgagga
     1081 gcacttcgag ttccacccgc tcgactacga ggacatcgcg tcgcgcaacc agcggccgaa
     1141 gatcgacgag tacgacgact acctcttcat cgtcctgcac ttcccggtgt tcgacaagag
     1201 cgtcgggcgc ctcaacgccg gcgagctcga catcttcatc gggcccggct acctgatcac
     1261 gctcccgaac gtcccgctcc agccggtcga gtacctgttc gagcgctgcc gggcctcgga
     1321 ggagacgcgc gagcagttct tctcgaaggg cgccggctac ctgctctaca agatcgtcga
     1381 cgacgcgttc gactactgct tcccgatgct gcgcaaggtc ggcaacaagc tcgaccgcgt
     1441 cgaggaggac atcttcgagg ggcgctccga ggaggtcgtg cgcgacatct cgaacgtcaa
     1501 gcaggagatc atcaacttcc gcaagatcat ccggccgcag cggccggtgc tccaggacct
     1561 cgagcggacc aagcagcggt tcctgagcga ggagcacgag ctctacttcg acgacgtggt
     1621 cgacgcctcc gagcgcatct gggacatgct cgagaactac aaggaggtgg tcgaggcgct
     1681 cgaggacacg aacgagtcgg tgctctcgca ccgggtcaac gacgtgctgc gcgtgctgac
     1741 cgccttcagc gtcgtgatcc tccccctcac gctgatcgcg agcctgtggg ggatgaacgt
     1801 gggcgtgccc ggcgagggct cgaaggccgc gttctggatc atcgtcgtgg cgatggtggg
     1861 gatgctcgtc tcgatgctca gctacttccg ccaccgcggc tggctctgaa gctccccgta
     1921 caggtcctcg tacgcggcac ccatacgctc ggtcgagaag tccgcggcgc gggctcggca
     1981 ggcctcgcgc gtggcggggt cggcggccag gtcgagcgct tcgaggaggg ctcgcgccag
     2041 tgcgtccggc tcgtcgccgc tgaacaggcg accgatcgtg tcgcggtcca ccacctcggg
     2101 gatggcgccc aggttcgacc cgacgaccgg cgtgccgcac gccagcgcct cgaccagcac
     2161 gagcccgaac gcctcccccc aggacgggag cgccgagacc caggcgctgc tgtagacctg
     2221 cgccagctcg gcgcgatcct gggggtcgat cagctcgatc ccgtcgcccg gccgcaggga
     2281 ttcgtggcgc tggagcagga gccgggcatc ggggcgctcg cggcgcacct ggcgaaaggc
     2341 gtcgatcagg agcgggaggc gcttgttctc ggctcccgca tcggccaggc agacgatcgt
     2401 aggctcgggc gctcgctccg cggtcggccg gaaggcgtcg agatcgaccg aggggtggat
     2461 cacgcgggcc tccaccccca acgcacgccg gaagccgtcg gccgcggtgc ggctgatcgc
     2521 gacggtcgcg tcgcactcgc gcgtggcgcg cacgagcagc tccgcccgca tgcgccgctg
     2581 ggcgatgccg gtgcgggtcg ggaggccgag gaaggtgaac accgacggca ggccactgcg
     2641 agcgccggcc acggcgtcgg gggggtgcgt ggcgtgggcg acgtcgaacg tgccgtgctt
     2701 caacgccagg tagctcagcg gcgcgtggct caggtagtcc tcgaacgcgc ggcggcgcag
     2761 gcgcgactcg accggcgggc gcgggaggcg caggatcggg aggccgtcct cgacggcccg
     2821 cgaaggagcc ccccggtgtg cggtgatcag gctcggctgg tggccgcggg cgatcaggtc
     2881 gtcggccagc tcgcgcacga gccgctcccc cccgcgccgc acctcgggga agaacgccgg
     2941 cgcgagcagc gcgatgcgga gccgctcggc gggctcgctc acaggaagaa cgtgcgccag
     3001 cggatgctgc cccgcgcgag ggctgccatc tcggcgctgt ccacgaccgc gcgcgccggc
     3061 agctccgaca gcgcgcgggc gcggccgcgc gggcccgcgc cgcggtcggg cagcgtgtgg
     3121 atcacccacg ggagcgccag cagctcggca ccgggcttgc gccggcgcag cagcgcaccc
     3181 gcaatcgcca gcggcagcca aacgtgggtg cgcttccaga agatccacat cgggaagtgc
     3241 tcccggacgc tcggctggcg cttcacgagg taggcgaggt gctgccagcg ccagcgcgcg
     3301 cgcacgagct gcgggagcga gaccgcgtgc acggcgtgga aggtcacgac gtcgcgcgcc
     3361 ccgacgtatc cggcgcccgc ctcggtcgcg cggatcgcga ggtcggtgtc ctcgccggcg
     3421 gcgagcggga aggtctcgtc gaagccgccg acgcgctcga gcagctcgcg cgggtagacg
     3481 atgttcgagg tctgcgccca cggcgtcggc ggcacgatcc gctgggtgtc cacgtgcggc
     3541 gccgcgcgga cgaggtgctc ctcgtcgggg tcggggatcg tggcgccctg gacgatcgcg
     3601 ccggggtggg cgcgcgcggc ggcgagcgca cgctcgagcc actcgggcgg cgggcggcag
     3661 tcgtcgtcgg tgaaggcgac gagcggcgcc gtggcggcgt gccagcccgc attgcgcttg
     3721 gcggccgggc cggggccggg ctcgaagccg atcgccctga cgcccagcgg atgcgtgcgc
     3781 agcaactcgg cggtctccgg cccgcgcgag tcgtgcgcga cgatcacctc gaagcgctcg
     3841 cgcggcagcg tctgctcctc gagcgcgttc agcagccagc gcagccgcag cgggcggtcg
     3901 tgcgagggga cgacgaccgc gatgtcgggt tggtccgcca ccgccccatc attaccgttc
     3961 cgccgcatgc cggcctggta ccacaccctc gagctcggcc cgggacacgt ggtcgagggc
     4021 atgttcgaca tgcgcccgta cgtggaccgc ttcggcctcc cggagcggat ggacgggatg
     4081 agggcgctcg acgtcggcac cttcgacggg ttctgggcct tcgagatgga gcgccgcggt
     4141 gccgaggtgg tggcgctcga catcgacgac gaggcggacc tcgactaccc gcctcggcgg
     4201 cgaccggcga gcttcggccc gcgcggggag aacttcaggc tggcgcgaga ggcgctcggc
     4261 tcgtcggtcg agcgagtcgg ctgctcgatc tacgacgccc ggccggagga gctcggcgag
     4321 ttcgacctcg tgctctgcgg gctcgtgctc gtccacctgc gcgaccagct cctcgccttg
     4381 gagcggatcg ccgggctctg ccggggcacg tttatctcgg gcgaggcgta cgacccgctg
     4441 atggacctgc tgccgttcgc cgcctcgcgc tacaaggccg accgcgacgc cgacgtggtc
     4501 ttctgggagc cggggtcgcg cacctggcgg cggatgatct ggtcggccgg gttcgaccgg
     4561 gtggagcggt gcgggcgctt caggctgccc tccacgcagg ggttctcggt gcgctgcgtc
     4621 gtgcaccacg cgcggccgtg agcgagccgc gggtgtcggt ggtcgtgccc acgcgcgacc
     4681 gggaggagcg gttgcggagg ctgctcgact cgctcgccgc gcagaccg
//
LOCUS       DASVPL010000157         6728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_99002, whole genome shotgun sequence.
ACCESSION   DASVPL010000157 DASVPL010000000
VERSION     DASVPL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99002"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2328)
                     /gene="uvrA"
                     /locus_tag="VF517_08085"
     CDS             complement(<1..2328)
                     /gene="uvrA"
                     /locus_tag="VF517_08085"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX8052937.1"
                     /translation="MAQSSELVVSGAREHNLRDVHVRLPRNALVVITGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGQMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVG
                     TVTEIYDYLRLLWARIGQPHCPNCDRPIEAQSAEQIVDQVMTMPEGSKFMVLAPVVRG
                     RKGEYGKLFEELRTEGYARVKVDGELRRLEDDIKLDKKYKHDISVVVDRLIMRGDLRK
                     RLADSIETAVAVAEGMIEVEDVDSGAITTYSERFMCLHCGTSMPELEPRMFSFNSPHG
                     ACPRCTGLGSQMEIDPDLIVPDPSLTLNDGAILPWTATSSNYYEQLTQAIADRYEIDM
                     DTPWEELPEDAQDCFLWGTDGDRIYINYRNRYGRRRSYTTAFEGIVHNLERRYKETDS
                     EYIREKIEEFMSVRPCPECKGARLRPESLHVRIGGLGVHEVTRMSARRAIGWFDELEM
                     TDTERQIARLIMREIDERLRFLDNVGVGYLSLDRAASTLSGGEAQRIRLATQIGSSLV
                     GVLYILDEPSIGLHQRDNARLIATLERLRDLGNTVIVVEHDEGTMRAADHVVDMGPGA
                     GEHGGRVVAEGTPEEVMRVAESATGQFLAGTREIPVPARRRRPSGYIEIQGATQHNLR
                     KVSAKVPLGVFCCVTGVSGSGKSTLVNEVLYKAVANRLHKAKLRPGAHRRVTGLEQVD
                     KIINIDQSPIGRTPRSNPATYTGLFDHIRELFSRTQESRARGYKPGRFSFNVKGGRCE
                     VCRGDGQIKIEMHFLPDVYVPCEQCGGRRYNRETLE"
     gene            2551..3594
                     /locus_tag="VF517_08090"
     CDS             2551..3594
                     /locus_tag="VF517_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX8052938.1"
                     /translation="MELALQDDGALVTQAYPKMKHDKAFPLLEQLQIRWVRVNIGWAA
                     VVGSKTANARTKPKTVKYDFSSYDTLINSARARGMQLEIGLTGNAPRWATGDKKKASP
                     YKPNAKLYAEFVRAAVNHFKGNVPRYSIWNEPNHTGWLTPLKSQASLYRALYLAGYKE
                     IKAVDPGAQVLIGETAPYASRRGVATPPIAFIKAMAKSGRLLADGFAHHPYDFNHAPT
                     YKFPGKDNVTLSGLDKLAKLLDSLAASNRLETPGGQPLDIYLTEFGHLRSGSQKQSEG
                     NRVKYLKKGYDMALAHPRVRQMLHFLLVQPAKRYRFFDTSLVSQTGGKTAAFKALVDW
                     AASNAGNVNGSRR"
     gene            complement(3615..4538)
                     /locus_tag="VF517_08095"
     CDS             complement(3615..4538)
                     /locus_tag="VF517_08095"
                     /inference="COORDINATES: protein motif:HMM:NF004526.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8052939.1"
                     /translation="MARETYSLQGKTVFITGAARGIGAECARRCAQKGAKVALVGLEP
                     DELPKVAAECGPEAIWFEADVRDTEALQAAVDGTVERLGGIDVCISNAGIAGGGPVRM
                     VPAEAIERIVEVNLIGSMRTLTACLPHVIERRGYILQVASVAAAIHSPGMSGYATSKA
                     GIEAYADCLRAEVKHLGVGVGVGYFSWIDTDLVRGADERRGYRTLRAKLRGPLAKTHP
                     VSAVGDAVLAGIESKSRWVVVPGWVRTLLYMRTVAQLLGEAQGAKNMPEAEQLAREEL
                     AEIGAEQASMPVGPGGRAAAESARGRAAARS"
     gene            complement(4549..5277)
                     /locus_tag="VF517_08100"
     CDS             complement(4549..5277)
                     /locus_tag="VF517_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052940.1"
                     /translation="MKVLQPTRLMREAAEVLQPVRDEVVVIGATALEAALWGTGARSA
                     ATTDVDCAVGMRSAAAVIDQLESAGLAPSEEPHERGFTWVRYGIKVQLIRPPARVANP
                     PVSRLVPNPHLSLTDRYREPVAFDDSPGEPRLLVARAAAILALKGQAFGRSRPSGGLV
                     ERDYHDAYLLVAHVGDEIAAEYNGTDDGQLRGLVRKTLDDLLGEQGREAVRNQATRLE
                     SDLSPREADVRLRRAVAGLRGALR"
     gene            complement(5274..6200)
                     /locus_tag="VF517_08105"
     CDS             complement(5274..6200)
                     /locus_tag="VF517_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8052941.1"
                     /translation="MRVLVADEDLGLRLTPVRVDTLTLDRAAEISAERGLIVRFRRAS
                     TDAKAALRARRIHYASDDGEIFVLDPPVAIQQSAPRRARAPADRAASGYDPFAMKASR
                     VSRWLLIHHEDVFTGRELARHTGLSEGAVSNIVRELHARVLVEVRPSPADARVRYVSL
                     PEPGRLLAAWERVWELRRVRLTDWDVGTTSTEDTLGVVRQASSPGLSWAVGGVAGAGL
                     VSRAVEAADVLVWIDPDQLSTWEDLLVPAPGRGSRGTLRLASAPDPFLFELAQDRDDL
                     RVADPAQLYLDCSREGERALEAADAVRRHMGW"
     gene            complement(6344..>6728)
                     /locus_tag="VF517_08110"
     CDS             complement(6344..>6728)
                     /locus_tag="VF517_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052942.1"
                     /translation="PEVVAEVEDVEAEAAAVDESDDTVAEPLLAHDDEPALAGDGDGA
                     DTAVEDEVAAEPEEEEEPEPEPAPTGGGGGGRSIRGAEGARLIALNMALNGTPRDETA
                     RYLSQNFELDDQDALLDEVYARVGG"
BASE COUNT          977 a         2419 c         2330 g         1002 t
ORIGIN      
        1 ctcgagcgtc tcgcggttgt agcggcgccc gccgcactgc tcgcacggca cgtagacgtc
       61 cggcaggaag tgcatctcga tcttgatctg gccgtcgccg cggcacacct cgcagcgccc
      121 gcccttgacg ttgaaagaga agcggccggg cttgtagcca cgcgctcgcg actcctgcgt
      181 ccgcgagaac agctcgcgga tgtggtcgaa cagccccgtg taggtagcgg gattcgagcg
      241 cggcgtgcgc ccgatcggcg actggtcgat gttgatgatc ttgtccacct gctcgaggcc
      301 ggtcacgcgc ctgtgcgcgc ccgggcgcag cttggccttg tggagccggt tggcgaccgc
      361 cttgtagagc acctcgttga ccagcgtgga cttgcccgag cccgacacgc cggtcacgca
      421 gcagaagacg cccagcggca ccttcgcgct gaccttgcgc aggttgtgct gcgtcgcgcc
      481 ctggatctcg atgtacccgc tcggccgccg ccgccgcgcc ggcaccggga tctcgcgcgt
      541 gcccgcgagg aactggccgg tggcggactc ggccacacgc atgacctcct ccggcgtgcc
      601 ctccgccacg acccgcccgc cgtgctcgcc cgcgccgggg cccatgtcga cgacgtggtc
      661 ggcggcgcgc atcgtgccct cgtcgtgctc gaccacgatc actgtgttgc cgaggtcgcg
      721 caggcgctcg agcgtcgcga tcagccgcgc gttgtcgcgc tggtggagcc cgatcgaggg
      781 ctcgtcgagg atgtacagca cgccgacgag cgacgacccg atctgcgtgg cgagcctgat
      841 ccgctgcgcc tcgccgcccg acagcgtgct ggcggcgcga tccagcgaga ggtagccgac
      901 gccgacgttg tccaagaacc tgagccgctc gtcgatctcg cgcatgatca gccgcgcgat
      961 ctggcgctcg gtgtcggtca tctcgagctc gtcgaaccag ccgatcgccc gccgcgccga
     1021 catgcgcgtg acctcgtgca ccccgagccc gccgatccgc acgtgcagcg actccggccg
     1081 caggcgcgcg cccttgcact ccgggcacgg ccgcaccgac atgaactcct cgatcttctc
     1141 gcgaatgtac tcggagtcgg tctccttgta gcggcgctcg aggttgtgga cgatcccctc
     1201 gaaggccgtc gtgtacgagc gccggcgccc gtagcggttg cggtagttga tgtagatgcg
     1261 gtcgccgtcg gtgccccaga ggaagcagtc ctgcgcgtcc tcgggcagct cctcccacgg
     1321 cgtgtccatg tcgatctcgt agcggtccgc gatcgcctgc gtgagctgct cgtagtagtt
     1381 cgacgaggtg gcggtccagg gcaggatcgc gccgtcgttc agcgtcagcg acgggtccgg
     1441 gacgatcagg tccgggtcga tctccatctg cgagccgagc ccggtgcagc gcgggcaggc
     1501 gccgtgcggc gagttgaacg agaacatccg cggctcgagc tcgggcatcg aggtgccgca
     1561 gtgcaggcac atgaagcgct cggagtaggt ggtgatcgcg ccgctgtcga cgtcctcgac
     1621 ctcgatcatg ccctcggcga ccgccaccgc ggtctcgatc gagtcggcca ggcgcttgcg
     1681 caggtcgccc cgcatgatca gccggtcgac cacgaccgag atgtcgtgct tgtacttctt
     1741 gtcgagcttg atgtcgtcct cgagccggcg cagctcgccg tcgaccttca cgcgcgcgta
     1801 gccctcggtg cgcagctcct cgaagagctt cccgtactcg cccttgcgcc cgcgcacgac
     1861 cggggcaagc accatgaact tggagccctc gggcatcgtc atcacctggt ccacgatctg
     1921 ctcggcggac tgggcctcga tcggccggtc gcagttcggg cagtgtggct ggccgatccg
     1981 ggcccagagc aggcgcaggt agtcgtagat ctcggtgacc gtgccgaccg tggagcgcgg
     2041 gttgcgcgag gtggtcttct ggtcgatcga gatcgccggc gagagcccct cgatcgagtc
     2101 cacgtccggc ttgtccatct gcccgaggaa ctggcgcgcg taggccgaga gcgattcgac
     2161 gtagcggcgc tggccctcgg cgtagatcgt gtcgaaggcc aggctcgact tgccggagcc
     2221 ggacaggccg gtgatcacca cgagcgcgtt gcggggcagg cggacgtgga cgtcgcggag
     2281 gttgtgctcg cgcgcgccgg atacgaccag ctcggaggac tgggccatcg gaggcaagtc
     2341 taggccgcgt gtcaggcggt ccgaggggcc gaaaagcgca aaatgcgcga ccggcgacgc
     2401 tcggcccatt cgtccctaga ctgcgagacg accgcccccg gactgcccct ggtgcccatg
     2461 actcgtcgcc ttcgcaggat cgcgctatcc ctttctctcg tcgccgtcgc cgctctcggc
     2521 gcggcctcgc catcgcaggc cgcccccggc atggagctcg cgctccagga cgacggagcg
     2581 ctcgtgaccc aggcataccc gaagatgaag cacgacaagg ccttcccgct cctcgagcag
     2641 ctccagatca ggtgggtgcg ggtcaacatc ggctgggcgg cggtggtcgg gtcgaagacc
     2701 gccaacgcca ggacgaagcc caagaccgtg aagtacgact tctcgtccta cgacacgctg
     2761 atcaattcgg cgcgcgcacg cgggatgcag ctcgagatcg gcctgacggg caacgccccg
     2821 cgctgggcca ccggggacaa gaagaaggcc agcccgtaca agccgaacgc caagctctac
     2881 gcggagttcg tgagggcggc ggtgaaccac ttcaagggca acgtcccgcg ctacagcatc
     2941 tggaacgagc cgaaccacac cggctggctc actccgctca agtcgcaggc atcgctctac
     3001 cgcgcgctgt acctggcggg ctacaaggag atcaaggcgg tcgacccggg ggcccaggtg
     3061 ctgatcggcg agacggcgcc gtacgcgagc cgcaggggcg tcgcgacgcc gccgatcgcg
     3121 ttcatcaagg cgatggccaa gagcggccgg ctgctggcgg acgggttcgc acatcacccg
     3181 tacgacttca accacgctcc cacgtacaag ttcccgggca aggacaacgt gacgctgagc
     3241 gggctcgaca agctcgcgaa gctgctcgac agcctcgccg cgtcgaaccg gctcgagacg
     3301 ccgggcggcc agccgctcga catctacctg accgagttcg ggcacctgcg gagcggttcg
     3361 cagaagcagt cggagggcaa ccgggtcaag tacctcaaga agggctacga catggcgctc
     3421 gcgcacccgc gcgtgcggca gatgctgcac ttcctgctgg tccagccggc gaagcgctac
     3481 cgcttcttcg acacgagcct cgtgagccag accggcggca agacggcggc gttcaaagcc
     3541 ctcgtggact gggcggcgtc caacgcgggc aacgtcaacg gctcgcgccg ctagcgggcc
     3601 ggtctagacc gagatcagga gcgcgcggcc gcccggccgc gcgctgactc ggccgccgcg
     3661 cggccgcccg gcccgacagg catcgaggcc tgctccgcgc cgatctcggc gagctcctcc
     3721 cgcgcgagct gctcggcctc gggcatgttc ttcgcgccct gcgcctcgcc gaggagctgg
     3781 gcgacggtgc gcatgtagag cagcgtacgg acccagcccg gcaccacgac ccagcgcgac
     3841 ttcgactcga tcccggccag gacggcatct ccgacggccg ataccgggtg cgtcttggcc
     3901 agcgggccgc gcagcttcgc ccgcagcgtg cggtagccgc ggcgctcgtc ggccccgcga
     3961 accaggtcgg tgtcgatcca cgagaagtag cccacgccga cgcccacgcc gaggtgcttg
     4021 acctcggcgc gcaggcagtc cgcataggcc tcgatcccag ccttgctggt ggcgtagccg
     4081 gacatgccgg gcgagtggat ggccgcggcc accgacgcca cctgaaggat gtagccgcgc
     4141 cgctcgatca cgtgcggcag gcaggccgtg agggtccgca tggagccgat gaggttgacc
     4201 tcgacgatgc gctcgatcgc ctccgcgggc accatccgca ccggcccgcc gccggcgatc
     4261 cccgcgttcg agatgcagac gtcgatgccg ccgagccgct cgaccgtgcc gtcgaccgcc
     4321 gcctggagcg cctccgtgtc gcgtacgtcg gcctcgaacc agatcgcctc ggggccgcac
     4381 tcggcggcga ccttcggcag ctcgtcgggc tcgagcccga ccagggctac cttggcgccc
     4441 ttctgggcgc agcgccgcgc gcactcggcg ccgataccgc gcgcggcgcc ggttatgaag
     4501 accgtcttgc cctggaggga gtaggtctcg cgagccatgg ggcggagcct aacgcagggc
     4561 gccgcgcagc cctgcgaccg ctcgtcgcaa gcgcacgtcg gcctcgcgcg gagagaggtc
     4621 ggactcgagg cgggttgcct ggttgcggac cgcctcacgc ccctgctcgc cgagcaggtc
     4681 atccagcgtc ttgcggacga ggcccctgag ctgtccgtca tcggtgccgt tgtactcggc
     4741 ggctatctcg tcgcccacgt gggcgaccag caggtaggcg tcgtggtagt cgcgctcgac
     4801 gagaccgcca ctcggccggc tgcgcccgaa cgcttggccc ttgagcgcga ggatcgcggc
     4861 cgcacgcgcg accagcagcc gcggctcccc cggactgtcg tcgaacgcca cgggctcccg
     4921 gtacctatcg gtgagcgaca ggtgagggtt ggggacgagc cgcgagacgg gcgggttggc
     4981 cacccgggcc ggcgggcgga tgagctgcac cttgatgccg tagcgaaccc aggtgaagcc
     5041 gcgctcgtgt ggctcctcgc tcggcgcgag gcccgcgctc tcgagctggt cgatcactgc
     5101 cgcggccgag cgcatgccga cagcgcagtc gacgtcggtg gtcgcggcgc tccgagcgcc
     5161 ggttccccac agggcggcct cgagagcagt cgcgccgatc accacaacct cgtcgcggac
     5221 gggctgcagc acctccgccg cctcacgcat gagccgggtc ggctggagga ccttcaccag
     5281 cccatgtgac gccggacggc gtcggccgcc tccagggccc gctccccctc ccgcgagcag
     5341 tcgaggtaga gctgggcggg atcggcgacc cgcaggtcgt cgcgatcctg tgcgagctcg
     5401 aagaggaacg ggtccggggc gctcgcgagg cgcaacgttc cgcggctacc gcggcccggc
     5461 gcgggcacga gcaggtcttc ccaggtggat agctgatcgg ggtcgatcca cacgagcacg
     5521 tccgccgcct cgaccgcgcg ggacaccagc ccggcgccgg ccactccccc gacggcccag
     5581 ctgaggccgg ggctcgaggc ctggcgcacg acccccagcg tgtcctccgt gctcgtcgtg
     5641 ccgacgtccc agtcggtcag ccggacccgc ctgagctccc agacccgctc ccacgcggca
     5701 aggagccggc cgggctccgg caagctcacg tatcgcacgc gcgcgtccgc gggactcggc
     5761 ctcacctcga ccagcacgcg cgcgtggagc tcgcgcacga tgttggacac cgcgccctcg
     5821 ctgagtcccg tgtggcgcgc cagctcgcgc cccgtgaaga cgtcctcatg gtggatgagt
     5881 agccagcggc tgacgcggct cgccttcatc gcgaacgggt cgtatccgga agcggctcga
     5941 tcggcgggcg cgcgcgccct ccgcggcgcg ctctgctgga tcgccacggg cgggtccagc
     6001 acgaagatct cgccgtcgtc tgacgcgtag tggatccgtc gcgcgcgaag ggccgccttc
     6061 gcgtcggtcg acgcgcggcg gaagcggacg atcagcccac gctcggccga tatctccgcg
     6121 gcgcgatcca gggtcagagt gtcgacgcgg accggcgtga gacgaaggcc gaggtcctcg
     6181 tcagcaacca gtacccgcac gctgtcgcgg ttctcgacga tgcgtgcggg tgttcccggc
     6241 gacgaatgta cttcatcagg acacatgctt tagtgcgcgg taaagcatac gcgtcactaa
     6301 agcttcgact ggagtctgaa cttacctgcc gtggggcggc ggcttagccg ccgacgcgcg
     6361 cgtagacctc gtccagcagc gcgtcctggt cgtcgagctc gaagttctgc gacaggtagc
     6421 gagcggtctc gtcgcgcggg gtgccgttga gggccatgtt cagcgcgatc aggcgcgcgc
     6481 cctcggcccc gcggatcgag cgaccgccgc ctccgccgcc ggtcggggcc ggctcgggct
     6541 ccggctcctc ttcctcctcc ggctcggccg cgacctcgtc ctcgacggcc gtgtcggccc
     6601 cgtcgccgtc gccggcgagt gccggctcgt cgtcgtgggc gagcagcggc tcggccacgg
     6661 tgtcgtcgga ctcgtccacc gcggcggcct cggcctccac gtcctcgacc tcggcaacca
     6721 cctccggc
//
LOCUS       DASVPL010000158         5731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_59087, whole genome shotgun sequence.
ACCESSION   DASVPL010000158 DASVPL010000000
VERSION     DASVPL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59087"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VF517_08115"
     CDS             <1..272
                     /locus_tag="VF517_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052943.1"
                     /translation="EAGQAQRVAAALPYLAARRRALRIERVYWYTWLTREVDPRYPFD
                     YAGLRRLEPDRVVEKPAFAAFRRTALRLQGSPSGAPSPTPPSSSP"
     gene            complement(211..1464)
                     /locus_tag="VF517_08120"
     CDS             complement(211..1464)
                     /locus_tag="VF517_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX8052944.1"
                     /translation="MTHHDETFARIPTHLALDPRDGDGKPVEEVRLLVRGQGTVGLAL
                     ARIATFTVAIGALDDSAAPAFRWSVTQTGATLSERAGGEWKEVAVALTPAAALDPEPN
                     CVYWFSFDAHNRTLGYGKGEMRLATRLASHEYPRPAKATDPDPYAWFGQATSVHVEPG
                     LERPTDVWRDPVTVEPALRVLPVDAISMKQMAQGTHTVAANLTTACQQLYSNVAGAQF
                     LLDTPDFPQFGEAIEESIASKDGWCRTTLDHKADEFGPHDPDKTYLRITMGVNQGESP
                     GIPFVVEIWPPGHYSPIHNHGGADAVIRVLRGRIHVSLFAMLSMKHQDAFGDAHFDEG
                     EVTWISPRLNQNHQLKNDGDITCVTIQCYQYAESDLTHYPYFDYIGEAEIGHFDPNSD
                     MDFLKFKAKMKEEWEKAHPTGSPGA"
     gene            complement(1497..3308)
                     /locus_tag="VF517_08125"
     CDS             complement(1497..3308)
                     /locus_tag="VF517_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052945.1"
                     /translation="MQVHVDFDLEGAGFLDPQDAAAKVGTALAGALATDKPALAAADP
                     ATIVAAGLPLGTLPTAANRVSGLVAGLTKAPHSLSAGAAAVLAAIVVAGLDRRSPGSL
                     PVAGDTVGSLFESGLAGADLASLDPLGLDVPAELTDLLAAAEPSAKHVTAEQIVAAGI
                     AAIVAGEPRFLNELTVSDFATAALSAALRIEDAAGAVVEGSRAIAGVAADPSLDGRIP
                     GGGFPGLAVVAGRVPTGLPIALSHLGPSRVRIRAPELADHFVDECGRDVAPTDLGCAL
                     AKTRLLTDLLNPDAAAEGWQLLEFDDPAVNHVFSHFHVLQDGLRFVLPEKQVKPFEQR
                     GLIGSWRLTVPALVNQSVVQLLPAIRDIRIVISTVARHDGNLVPLVKQPALSLPEPPI
                     PVGSAPGQVNAAFNDALADLGKLFEDLLGDLKGQIDGTLADLTAKVGQLGSGVTSLID
                     VASVLSAATAQLAALAGVAAPGAGVRMVTLDKASLSNPTAFLAGGGTATSTTFTITQA
                     MLTTANATGTLKGVLGVIVSPTPVPPPVGTITFPTALGKLTAPDGSVHNIKIDSGLAF
                     VDVPDTTGALGLWKIELPAGMPALTNLGVGVLVQLLP"
     gene            complement(3236..>5731)
                     /locus_tag="VF517_08130"
     CDS             complement(3236..>5731)
                     /locus_tag="VF517_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052946.1"
                     /translation="GPEPDPVPWHERRDLAVQPGELAKAVDLLGGGQVDPGRVEEERV
                     RTLLAGPVGADELGEALAVAQSLAAGGDARRAAQLAAHAARGAAQARNLTAVQRYQLN
                     AIAPAVALAATPGYDVRSVEATASAVLRGAVPGTRAAREAVRAATRARAHTARRRVAR
                     AGGPSSPSARQAELAARADRMALLTGAGLDDDVQAKSALGLQTAAVLIDVMRATGATV
                     MHGARPGVIDNAADPGAHGRVLPANGARHMVGAGGVASLGDPFLKGQIAGTILDLKKH
                     KTSALDGLLAATEGDLFRFDYLLGEARRLVDDLTGIEGLLFGLLDTVVEQASAAADSV
                     DDLLSRLLEELTAQIARLADQLQGLILGLAASLRDELGLMAFVEIIANWPHENWIGLF
                     EGTRTHLSDPDGPGLEVAIERALEDMLQGEIAGVVNGVEAEIRKKLTELNDRWKKLVL
                     EPDAQTEALGEVNTLLGEIDAKLAQLGVSGASGQIRAALNDALGEIDIVKLAEPVLSS
                     LRGPVALPGWARVLLVGYIAAPFIAAIVFVIIPLILASGLVIAAVFAGQTWTIGLLTG
                     LGKFLLEGVLSAIIAFLVVAGVQAVIALIARFVISKILALGVEINNEVARVLGLLDAR
                     FSQIVDEILRMSMLEGLLDAWKAELGLLGGILPRQAVELLHTLLTGARDTIESRLDRL
                     LCAAERSFFRETLELVGLQAAEKAPVVPATATIAGLSDPRLGTSARYASVLSAFEHRR
                     VTAGLDPPIKLTHSFSLRQLLERPASEPPVSVPHLFHDLLAGQAVDFTITQETVDRCS
                     PARTASSSRTCRCTWTSTSRARASWTRRTRRPR"
BASE COUNT          753 a         2063 c         2113 g          802 t
ORIGIN      
        1 acgaggccgg ccaggcgcag cgcgtggccg ccgctctgcc ctacctcgcc gcgcgccgcc
       61 gcgcgctgcg catcgagcgc gtctactggt acacgtggct cacccgcgag gtcgacccgc
      121 gctacccgtt cgactacgcc ggactccggc ggctcgagcc cgaccgcgtg gtggagaagc
      181 ccgcgttcgc ggcgttccgg cgaaccgcgc tcaggctcca ggggagcccg tcgggtgcgc
      241 cttctcccac tcctccttca tcttcgcctt gaacttcagg aagtccatgt ccgagttcgg
      301 gtcgaagtgc ccgatctcgg cctcgccgat gtagtcgaag tacgggtagt gcgtgaggtc
      361 ggactcggcg tactggtagc actggatcgt cacgcaggtg atatcgccgt cgttcttcag
      421 ctggtggttc tggttgagcc gcggcgagat ccaggtcacc tcgccctcgt cgaagtgcgc
      481 gtcgccgaac gcgtcctggt gcttcatcga cagcatcgcg aacaggctca cgtggatccg
      541 cccgcgcagg acccttatga cggcgtccgc cccgccgtgg ttgtggatcg gcgagtagtg
      601 acccggcggc cagatctcca cgacgaacgg gatccccggc gactcgccct ggttcacgcc
      661 catcgtgatc cgcaggtagg tcttatcggg atcgtggggt ccgaactcgt ccgccttgtg
      721 gtcgagcgtc gtgcggcacc aaccgtcctt gctcgcgatg ctctcctcga tcgcctcccc
      781 gaactgcggg aagtccggcg tgtcgagcag gaactgcgcg cccgcgacgt tcgagtagag
      841 ctgctggcag gccgtcgtga ggttcgcggc cacggtgtgc gtgccctgcg ccatctgctt
      901 catcgagatc gcgtcgaccg gcagcaccct cagcgcgggc tcgaccgtga ccgggtcgcg
      961 ccagacgtcc gtcggccgct cgaggcctgg ctcgacgtga acgctcgtgg cctgcccgaa
     1021 ccacgcgtac gggtcggggt cggtcgcctt cgccggccgc gggtactcgt gcgacgcgag
     1081 cctggtcgcc agccgcatct cgcccttccc gtagccaagc gtgcggttgt gggcgtcgaa
     1141 gctgaaccag tagacgcagt tgggctcggg gtcgagcgcg gcggccggcg tcagggccac
     1201 ggccacctcc ttccactcgc ctccggcgcg ctcgctcagc gtcgcgccgg tctgggtcac
     1261 gctccagcgg aacgcgggcg cggcggaatc atcgagcgcg ccgatcgcga cggtgaacgt
     1321 tgcgatccgg gcgagcgcga gccccaccgt gccctggccg cggacgagca ggcgaacctc
     1381 ctcgaccggc ttgccgtccc cgtcccgcgg gtcgagcgcg agatgcgtgg gaatacgagc
     1441 gaaggtctcg tcgtggtggg tcatgcgcgg ctgccggcca ccctaaccga cggagctcag
     1501 gggagcagct ggacgaggac gccgacgccc aggttggtga gcgcgggcat ccccgccggc
     1561 agctcgatct tccacaggcc gagggcaccc gtggtgtcgg gcacgtcgac gaacgccagg
     1621 ccgctgtcga tcttgatgtt gtggacggag ccgtccggcg ccgtgagctt gccgagcgcc
     1681 gtcgggaagg tgatcgtgcc gaccggcggc ggcacgggcg tcggcgagac gatcaccccg
     1741 agcacgccct tcagcgtgcc ggtggcgttg gccgtggtga gcatggcctg cgtgatcgtg
     1801 aaggtggtgc tggtggcagt gccgccgccg gcgaggaacg cagtcgggtt cgagagcgag
     1861 gccttgtcca gcgtgaccat ccggactccg gcgccgggtg cggccacgcc ggcgagcgcg
     1921 gcgagctgag ccgtcgccgc gctcagcacc gacgcgacgt cgatcaggct cgtcacgccg
     1981 ctgccgagct ggcccacctt cgcggtgagg tcggcgagcg tgccgtcgat ctgccccttc
     2041 aggtcgccga gcagatcctc gaacagcttg ccgaggtccg cgagcgcgtc gttgaacgcg
     2101 gcgttcacct gacccggcgc cgacccgacc gggatcggcg gctccgggag ggacagcgcc
     2161 ggctgcttga cgagcgggac gaggttgccg tcgtggcgcg cgacggtgct gatcacgatc
     2221 ctgatgtcgc ggatggccgg caggagctgc acgaccgact ggttcacgag cgccgggacc
     2281 gtcagccgcc agcttccgat cagcccgcgc tgctcgaacg gcttcacctg cttctcgggg
     2341 agcacgaagc gcaggccgtc ctggagcacg tggaagtgcg agaagacgtg gttgaccgcc
     2401 gggtcgtcga actcgaggag ctgccagccc tcggccgcgg cgtcggggtt gagcaggtcg
     2461 gtgaggagcc tcgtcttggc gagggcgcag ccgaggtcgg tgggcgccac gtcgcggccg
     2521 cactcgtcca cgaagtgatc cgcaagctcg ggcgcgcgga tccggacgcg cgaggggccg
     2581 aggtgcgaga gcgcgatcgg cagcccggtg gggacccggc ccgcgacgac cgcgagcccc
     2641 gggaagcctc cgcccgggat gcgcccgtcg agcgacgggt cggcggccac gccggcgatc
     2701 gcgcgcgagc cctccacgac ggcgccggcc gcgtcctcga tccgcagcgc cgccgacagc
     2761 gcggcggtgg cgaagtcgct cacggtgagc tcgttcagga agcgcggctc gcccgccacg
     2821 atcgccgcga tcccggcggc cacgatctgc tcggcggtca cgtgcttcgc cgagggctcg
     2881 gcggccgcga gcaggtcggt gagctcggcc ggcacgtcga gcccgagcgg gtcgagcgac
     2941 gcgaggtcgg cgccggcgag gccgctctcg aacagcgatc cgaccgtatc cccggcgacg
     3001 ggcagcgaac cgggggagcg gcggtcgagc ccggccacca cgatcgcggc cagcaccgcg
     3061 gcggcgcccg ccgagagcga gtgcggcgcc ttggtcagcc cggcgaccag gccgctcacg
     3121 cggttggccg ccgtgggcag ggttcccagc ggcagcccgg ccgcgacgat cgtggccgga
     3181 tccgcggcgg cgagcgccgg cttgtcggtc gcgagcgcgc ccgcgagcgc ggtgcctacc
     3241 ttggccgccg cgtcctgcgg gtccaggaag cccgcgccct cgaggtcgaa gtccacgtgc
     3301 acctgcacgt ccttgatgag gacgcggtgc gcgccgggga gcagcggtcc acggtctcct
     3361 gtgtgatcgt gaagtcgacg gcctggcccg cgagcaggtc gtggaacagg tgcgggaccg
     3421 agaccggcgg ctcgctcgcc gggcgctcga ggagctggcg caggctgaag ctgtgcgtga
     3481 gcttgatcgg cgggtcgagc cccgcggtca cgcgcctgtg ctcgaacgcc gagagcacgc
     3541 tcgcgtagcg cgcgctggtc ccgaggcgcg ggtcggagag cccggcgatc gtggcggtgg
     3601 ccggtacgac cggcgccttc tcggccgcct ggagccctac gagctcgagc gtctcgcgga
     3661 agaacgagcg ctccgccgcg cacagcagcc ggtcgagccg cgactcgatc gtgtcgcgcg
     3721 cgcccgtgag gagcgtgtgc aggagctcga ccgcctggcg cggcaggatg cccccgagca
     3781 gcccgagctc cgccttccac gcgtcgagga gcccctcgag catgctcatc ctcaggatct
     3841 cgtcgacgat ctggctgaag cgcgcgtcga gcagcccgag cacgcgcgcg acctcgttgt
     3901 tgatctcgac gcccagcgcg aggatcttgg agatcacgaa ccgcgcgatg agcgcgatca
     3961 cggcctggac gcccgccacg accaggaacg cgatgatcgc gctgagcacg ccctcgagca
     4021 ggaacttccc gagccccgtg agcaggccga tcgtccaggt ctgaccggcg aagaccgccg
     4081 cgatcaccag gcccgaggcg aggatcagcg ggatgatcac gaacacgatc gccgcgatga
     4141 acggcgccgc gatgtagccg acgagcagca cgcgcgccca gcccggcagc gcgaccgggc
     4201 cgcgcaggct cgacaggacg ggctcggcga gcttgacgat gtcgatctcg cccagcgcgt
     4261 cgttcagcgc ggcgcgtatc tggccgctcg caccgctcac acccagctgc gcgagcttcg
     4321 cgtcgatctc gccgagcagc gtgttgacct cgccgagggc ctccgtctgc gcgtcgggct
     4381 cgaggacgag cttcttccag cggtcgttca gctccgtgag cttcttgcgg atctcggcct
     4441 cgacgccgtt gacgactccg gcgatctcgc cctggagcat gtcctcgagc gcgcgctcga
     4501 tcgcgacctc gaggccgggg ccgtcggggt cgctcaggtg cgtgcgcgtg ccctcgaaca
     4561 ggccgatcca gttctcgtgc ggccagttgg cgatgatctc gacgaacgcc atcagcccga
     4621 gctcgtcgcg cagcgaggcc gcgagcccga ggatcagtcc ctgcagttgg tcggccagcc
     4681 gcgcgatctg agcggtcagc tcctcgagca ggcgcgagag gaggtcgtcg acgctgtcgg
     4741 ccgccgcact cgcctgctcc acgaccgtgt ccagcaggcc gaacaggagg ccctcgatgc
     4801 cggtcaggtc gtccacgagc cggcgcgcct cgccgagcag gtagtcgaac cggaacaggt
     4861 cgccctcggt ggcggccagc agcccgtcga gcgcgctcgt cttgtgcttc ttgagatcga
     4921 ggatcgtgcc ggcgatctgg cccttcagga acgggtcccc gagcgacgcg acgccgcccg
     4981 cgccgaccat gtggcgcgcg ccgttcgcgg gcagcacgcg gccgtgggcg ccggggtccg
     5041 cggcgttgtc gatcacgccc gggcgcgcgc cgtgcatcac ggtcgcgccc gtcgcgcgca
     5101 tcacgtcgat cagtacggcg gcggtctgga gccccagcgc gctcttggcc tgcacgtcgt
     5161 cgtcgagccc cgcgccggtg agcagcgcca tccggtcggc gcgcgcggcc agctccgcct
     5221 ggcgcgcgct cggcgagctc ggcccgccgg cgcgggccac ccggcgccgc gccgtgtgcg
     5281 cgcgggcgcg ggtcgccgca cgtaccgcct cgcgcgccgc gcgcgtgccg ggcaccgcgc
     5341 cgcgcagcac ggccgaggcg gtggcctcga ccgaccggac gtcgtagccc ggcgtcgcgg
     5401 cgagcgcgac cgccggagcg atcgcgttca gctggtagcg ctggaccgcg gtcaggtttc
     5461 gcgcctgcgc ggccccgcgg gcggcgtgcg cggcgagctg cgcggcgcgg cgggcgtcgc
     5521 cgccggccgc gagcgactgg gccaccgcca gcgcctcgcc cagctcgtcg gctccgaccg
     5581 gtccggcgag cagcgtgcgc acgcgctcct cctcgacccg cccggggtcc acctggccgc
     5641 cgccgagcag gtcgacggcc ttcgcgagct cacccggctg gacggcgagg tcgcgccgct
     5701 cgtgccacgg gacggggtcc ggctcggggc c
//
LOCUS       DASVPL010000159        16865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_332155, whole genome shotgun sequence.
ACCESSION   DASVPL010000159 DASVPL010000000
VERSION     DASVPL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16865
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332155"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..3021
                     /locus_tag="VF517_08135"
     CDS             109..3021
                     /locus_tag="VF517_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8052947.1"
                     /translation="MFRRSSMRGIALAAVSASALLAGGGIAHASTVANSSGPITYNAA
                     AGEANDVSVALSGANLLITEAGAGVTMTDGDFADACTVAGNVATCPAAPSGLTVNLGD
                     LDDKFTAAGQTLVAGFGQFLTVSGQDGADTLTGGEGADLLYGDSQSPGSGDGADTISG
                     RDGNDLIHGHAGNDVLNGGAGSDFVQGYADDDTIDGGPGADGLVFVGFSGCAVPGFGS
                     YPGGLNGGPGNDTIDGSAGEDLLCGGGGTDTLRGDEDRDSLNDGDVTGSAHPATTRTV
                     DADTLDGGAGDDYMDYSGRTRGVQVTLADGLVNEGEVAGPGNVPAAENDVVTDVEDVA
                     SGSGNDSLTGSAASNDFYSGSGNDTLTGLAGSDYLNAAGPVATGLTDDDFVDGGGDDD
                     SISTGDGADTAIGGAGNDGLATGGGTDSAFGDAADDSGTGDDSIEAGFGDDTVRGSGG
                     NDLLDGGVRFPFFPFSPPSTESDVDDVRGGAGNDTVRGGGVGTDSLLGGEGDDSLGDG
                     DMRESPASAQRVDADVQDGGPGTDSVDYGFRADDVVLTLGDDAANDGEAADGSRPPEN
                     DTVRNVENAKTGSGDDVLTGTAGPNSLEGGEGGDSLEGAGGPDVLGGQGGGDTASGGA
                     GDDQVSGGAGDDTTSGGSDDDEISGGGGTDTVNGDDGHDVLHDGDAIGMPDADVLNGG
                     SGRDVVSSYEARRASVTVDLGGAAVSGVAGENDVLTGLENAATGLGDDTLTGSSSANH
                     LAGGRGDDTISGGDGSDELQGGFGADTLTGGGASDALDGGEGPDTLNSGDGGPDHDGC
                     GADVDTVNGDGFDTVDGDCENLTGGATRSGPGPQGPAGGTGPAGGTGPAGGTGPAGTQ
                     GPAGPAGPAGPQGTPGPQGPAGPAGASGGKVACKLRGRRITCTFTPTKRKGASRVVVV
                     LRRGGVKVARGSATVRGATQVRMTAKRRPAPGRYSIAVVVYGDGSTVRLDGSTTLR"
     gene            complement(3023..3799)
                     /locus_tag="VF517_08140"
     CDS             complement(3023..3799)
                     /locus_tag="VF517_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8052948.1"
                     /translation="MSQPNVSERVGIAGTGAIACGLARLAAPLGAVVVWARSPESAAR
                     ARDEIRCGVGVTTDLADLSGCTYVVEAVVEDAAVKGDLLRELDAATDDGAILASTTSS
                     LSVEGLARAAGRPDRFAALHVFNPVERMALVELAFPPAAADATRARTRALCEALGKTP
                     IEVPDAPGFVVNRLLFPYLFAAVRLVEEQGIEPEAVDACMKLGAGHPTGPLKLLDYVG
                     LDVAAAIGESIGETVPERVRELVAAGRLGRKSGAGFYDYG"
     gene            3878..5077
                     /locus_tag="VF517_08145"
     CDS             3878..5077
                     /locus_tag="VF517_08145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX8052949.1"
                     /translation="MQNGTGREVVIVEAVRTPIGRGHPEKGYYKDTHPNALLAKCYTE
                     VIERAGIEPTEVEDVITGCVQQYGEQMFNVGRNAWLQANLPVETPATTVDRQCGSAQQ
                     AVNFGAALVAAGVHDAVIGSGVEHMGHIPMGVGFTWVDQVGSPWPPELLEHYNLVPQG
                     ISAEMIADKWEIPRSELDEIGVRSHQNAARATEEGRFEREIVPFQVNGDTYVTDQGIR
                     PDTNLEKLSGLKVAFPDAASHVGGEENLKVTAGNSSQISDGAAAILLMTREKADALGL
                     KPRARILDQTTVGVDPVIMLTGPIPATTKLLERNKMKIDDIDLFEVNEAFASVLAAWR
                     REHEPDWDRVNVNGGAIALGHPLGSTGARLITTLLHELERSDKEVGLVTMCCGGGLGT
                     GTLIQRI"
     gene            5112..6905
                     /locus_tag="VF517_08150"
     CDS             5112..6905
                     /locus_tag="VF517_08150"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8052950.1"
                     /translation="MTPTFTQTVGGRYRVVRRLGAGGMATVFLAEDERLGRQVAVKRL
                     HGESTEEVARRFRREARLGAALNHPNVVAVYDITADDEGVLIVMEYVEGQTLRDEIGD
                     GPLPPERAIAILRCIAKALDYAHENGVVHRDVKPANVLIRRDGVVKLADLGIATAAEQ
                     SKITRSGSVLGTAAYMAPERLDGRPGGPAVDVYALAAVAFEMLCGRKAYEGNTPLEVA
                     HKVVNEPTPDLRRCVESAPEPLAEALKLGLAWEPDARPARAGDLVAAIEAALPEAAAS
                     AAVEPSADAPAAHVFAPEAEAASASADGGSEEQGTPVRVLPPERTRTRPARTAAAAAP
                     AAPAPTTAPAAQPAPTPDVGQVRGQIRPKDGPVGERGAETSAAGGSAGRPERGRRLGL
                     PVIGAALASVALLVILIVALAGGGSGDTEGDRVDKGDTKGATAEKGSSGDESSNGENG
                     SSGAQGSNGEGSTGSEGGSGGSAPADTGSGGGSTPAAGTAAAAVNDFYQRAARGDYAG
                     AWALATPRAQEKLGGFSSFSAGQSTLDTISFPKLQGKESGDTATVTLQSRAVHRDRID
                     RCRGSVDLVRSGGAWKLDDFHIATCAKSPRP"
     gene            7043..9700
                     /locus_tag="VF517_08155"
     CDS             7043..9700
                     /locus_tag="VF517_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8052951.1"
                     /translation="MGAVSRLLSIATEAERPDALARALVREARAELRAPAAVVFAIDQ
                     RSGRAVVAAAEPQTPHREWFAVDDASPLADALEAGAPVVLSTAQARELALASGLQPRP
                     QAALLAPMPHPGGALAYALMVGDDRTRVFEADELEAAGALAEAARASMAQRQLAERHG
                     TERARQAAIARAAKALNESLDLTRVLPRICEEAAAILEADRVSVFRGSAGNPLTLEAT
                     NQTPEAGPAAPEPGGGISAKALDLGRPILTNDYARDGAPTVGSPFAAIRGAIAVPMRW
                     DGELRGVLWTGWTSDRLMTDDDARLLESFAELAAVACRNASVHADLAEAARTDGLTGC
                     MNHAALHETLRREMQRARRTGRPLTVVLVDLDNFKQVNENQGHLVGDEVLRRVGRALR
                     QAVRPYDFVARYGGDEFAIVSVDSEEEGGAEIARRAIQRLSESVAEIGHDKAATFATA
                     GVAEWTTALSPSELLRVADRALLYGKQQGVRGKAIPASSVPEDFRLGRGGAPEQVEAT
                     PPAQLPGSQALDGEPLRRRNRQLTLANALGARLSEMTVVDEIVEAAVEELHRAFGYFT
                     CAVVRRSEDGESIDLLAGRGAAYQEVTAWRQPIAAGVIGRCLRERRTIVVNDVSRDSD
                     YFATAATEAVRSELVSPIWVGPELWGALDVQEVNEGAFDEADARLIETVTDQLGAALR
                     SAILYEQLERAYLGTAEALATALEAKDAHSASHARSIVSNAEAVARLLGLGGQDLRDV
                     RYGAAFHDIGKIAVPEGILGKPGPLSDSERAQVERHVLIGEQILAPVEFLSRVRTIVR
                     HGHERWDGTGYPDRLAGDEIPLGSRIVLACDALDAMTTDRPYREALPRNAARSELRLH
                     AGSQFDPRVVDALLEVLDRREAPTLESAK"
     gene            9750..10241
                     /locus_tag="VF517_08160"
     CDS             9750..10241
                     /locus_tag="VF517_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052952.1"
                     /translation="MAESKQAIAYEAATRALSDQRASLDNLRSRAASLVAAAAIVTSF
                     LGAAALADTRLTRATGTAQFVPDRSLQPAEAVALGAFLLVLFLCLLILRPRGKWKFVL
                     GAKWILERDEKDEDALMKLLAEWSETYWKRNNKWIKRRMDMFQVGVLLLGVESVAWVV
                     DLA"
     gene            10252..10419
                     /locus_tag="VF517_08165"
     CDS             10252..10419
                     /locus_tag="VF517_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052953.1"
                     /translation="MTWTEREPDGPAGESDDDGPGLEETSALEEADSDPAPADDDGPG
                     LTEDYDQEGPA"
     gene            10492..11631
                     /locus_tag="VF517_08170"
     CDS             10492..11631
                     /locus_tag="VF517_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8052954.1"
                     /translation="MDLTLSPQEQAFRDELRAWLEENHPGEEPEGDESAGFEFRRDWQ
                     RKLAEARWAGLSWPEEYGGRGATLIEQAIFNEEIVRAKSPQMANVLGLVMGGPVVIAH
                     GTDEQKQRYLKPILTADEIWCQGFSEPESGSDLASLKTKAVKSNGEWIVTGQKVWTTF
                     AHEAKWCMLVARTDMDAPKHKGLTYFLMDMEQEAVQVRPLRQITGEAEFNELFIEEAR
                     IPDENIVGGEGNGWMVAITTLMHERAGLALGLQVQLKIVIGELMEEIRERGLEDDPII
                     RQRLAQIYIESECVRLNASRGLTRTMKTGIPGPEGSLTKWQWSDANQALTELAMDVRG
                     PDAPVIDGDWTYRFLRARANSIEGGTTEILRNIVAERVLGLPKLR"
     gene            11636..12652
                     /locus_tag="VF517_08175"
     CDS             11636..12652
                     /locus_tag="VF517_08175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8052955.1"
                     /translation="MNFDYSDDQQAIKSTARDFLASRFKLEKVRELAIAGEYDDGLWG
                     QIADLGWPGIFIDEAHEGQDLGVIELAILQEELGYACAPTPFFSNAAAGLLVKHGGSS
                     EQHERLLPGVASGEARGTVGIVKDGAAPLVPDADTASFVVLLDNGTGQIVETASAQIE
                     KVDTIDSTRRFFRVSADGGDSLEGDLSAGFQRIEVALAAELVGLAQRALEMSVEYAKD
                     RKQFDRPIGAYQAVSHKCAQMMLETEGARAATLYAAWAADHEPESLPLGASMAKAYAA
                     DAGWRVTAAALQVHGGIGFTWEHDLHWFLKRARTNGALFGSAREHRDRVAERIGLSRS
                     AAAA"
     gene            12690..13403
                     /locus_tag="VF517_08180"
     CDS             12690..13403
                     /locus_tag="VF517_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8052956.1"
                     /translation="MSPFDRLRRLMPGGGEPDLDAELRWWLDEWEPIVRDGGFNPGDV
                     PAFLPEGEPVADTFEGRRWQIARAEVARVLTEAGIEDEDFFEGKVVVDIGSGPLGFPD
                     ACPAKLAIAVEPLADRYRAAGLLLGGENTVYLATGAEAIPLLSDSTDVVLTRNSLDHV
                     SNPTAVVNEILRILRPGGTLILSVDIGHPATATEPHSFTRGEIRMLLNPLDIVSERTL
                     DEAHGGGGGSRIVVVARRR"
     gene            13441..14619
                     /locus_tag="VF517_08185"
     CDS             13441..14619
                     /locus_tag="VF517_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052957.1"
                     /translation="MPSRLIPLALLLVALLAPARASAQQRQVPQGYFGAVADGVLTDE
                     DPQRFENELPRMVGAGVESLRMVFPWRDAQPYKTRAEIPSDQQAFFRDEGGVPTDWRL
                     LDGYVEHAARQGLTILPVVLYAPQWAAKYGNSDASPPRGHTDYARFVVAVAKRYGRSG
                     TFWTERPDVPRLPIDWLQIWNEPHFKEYWSDQPWEADYARLLRGTYRAVKKEVPSAKI
                     LVAGMANKSWTYISKLYAQKTRGYFDGIAMHPFTNSIDGVVEIIERGRRVMARNGDGK
                     LPLMITEMSWTSAKGKAGYTYGNERTEAGQAKALRQGLQRMAKDRRALRILHVYWYTW
                     MTRDKHENIPFDYAGVIRFESDGTTTEKPAFAAYRSTAMALEGCAGKPSGARSCTPRR
                     "
     gene            complement(14639..14872)
                     /locus_tag="VF517_08190"
     CDS             complement(14639..14872)
                     /locus_tag="VF517_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052958.1"
                     /translation="MSSGSPRLLVIFTAATALVVGLIAALFTDNWIFLPLALGIHAIG
                     TAAVLMAIVPRLKDEDKPDPVTEARLEEERAAR"
     gene            complement(14922..15710)
                     /locus_tag="VF517_08195"
     CDS             complement(14922..15710)
                     /locus_tag="VF517_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8052959.1"
                     /translation="MGRAFTRLPPALDRALPLLAPWAREDGRLREEGYLDLVGDAAPR
                     STGVTQDLMVSRVVPAIYERWWRPALGRVAKGVLGPGMQDEHRIARLLLGLSPGDGVL
                     DVACGTGNFTREFARTVGPDGLAVGIDVSETMLARAVTDTDAAALLDRTAFVRGDAVE
                     LPFVESSFDAVCCFAALHLFEDPMRALDRMTEVLTPGGRIALFTSAQGRTALMRPWAS
                     MAGRRSGMRMFELGEVVRALEARGYGNVQQRVAGVTQFVGGRLL"
     gene            complement(15721..15996)
                     /locus_tag="VF517_08200"
     CDS             complement(15721..15996)
                     /locus_tag="VF517_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052960.1"
                     /translation="MSEFEKPDRVGREPTVEDVRELMGPSTPHFAPHIRNRIRNLIGG
                     LPEGHPARVEGEMQIERLNGIAFSGEQRGPAREGERSLPSVRGDEPA"
     gene            complement(16021..>16865)
                     /locus_tag="VF517_08205"
     CDS             complement(16021..>16865)
                     /locus_tag="VF517_08205"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX8052961.1"
                     /translation="SDGGDLPAVTKDALARLKPRGQTLAKGAQVILVGDKPPAPDGFK
                     SGAIKGSDPYAIAAAIDRFQTTAAGKPTPDVLVVSGEDPAYAMPAASWAARSGTPVLF
                     VKKNEVPQATRQAIQQHSKPNIYVLGPPSIVSPATAKQLDALGTVVRIGEKATNPVES
                     AVEFTAFNSKGFGWGAVVPGRNYALANASRPLDAAAAAALGSNGVYAPLLVTDRGDAL
                     PKPLENYLLDVQPGFENGDPSQGLYNRVWVLGNEDAVATGVQARVDELLQLIPVDRSP
                     DSGS"
BASE COUNT         2497 a         6057 c         6256 g         2055 t
ORIGIN      
        1 gaaccggctg gcacatcggc gcttgacaaa gcgccgcggc gcaaacagga ttgagcattg
       61 gattcgcgct gcgcccggac tggcccgggc gggggaaagg cagggctcgt gttccgtcgt
      121 tcatcgatgc gcggcatcgc gcttgcggcc gtgtccgcgt cggctctact cgccggcggc
      181 gggatcgcgc acgcgagcac cgtcgcgaac tcgtccggcc cgatcacgta caacgccgcg
      241 gcgggcgagg cgaacgacgt ctcggtggcg ctgagcggag cgaacctcct gatcaccgag
      301 gcgggcgccg gggtcacgat gaccgacggg gacttcgcgg acgcctgcac cgtcgcgggc
      361 aacgtggcca cgtgccccgc cgctcccagc ggcctgacgg tcaacctcgg ggacctggac
      421 gacaagttca ccgcggcggg acagaccctg gtggcgggct tcgggcagtt cctcacggtc
      481 agcggtcagg acggggccga cacgctcacc ggcggcgagg gggccgacct cctctacggc
      541 gactcgcaga gccccgggtc gggcgacgga gccgacacga tctccggccg cgacgggaac
      601 gacctgatcc acgggcacgc ggggaacgac gtcctgaacg gcggcgccgg ctcggacttc
      661 gtccagggct acgcggacga cgacacgatc gacggcggcc caggtgcgga tgggctcgtg
      721 ttcgtcggct tctcgggctg cgccgtgccg ggcttcggct catacccggg cgggctgaac
      781 ggcggaccgg gcaacgacac gatcgacggc agcgccggcg aggatctgct gtgcggcggc
      841 ggcggcacgg acacgcttcg cggcgacgag gaccgcgact ccctcaacga cggcgacgtg
      901 accggctcgg cgcaccccgc gacgacccgc acggtggatg cggacacgct cgacggcggc
      961 gccggagacg actacatgga ctacagcggc cgcacgcggg gggtccaggt cacgctcgcc
     1021 gacgggctcg tgaacgaggg cgaggtcgcg gggccgggca acgtccccgc ggccgagaac
     1081 gatgtcgtga ccgacgtgga ggacgtcgcc agcggctcgg gcaacgacag cctgacgggc
     1141 agcgccgcgt ccaacgactt ctactccggc tcggggaacg acaccctcac ggggctcgcg
     1201 gggagcgact acctcaacgc ggccggtccg gtggccaccg gcctgaccga cgacgacttc
     1261 gtggacggcg gtggggacga cgacagcatc tccacggggg acggagccga caccgcgatc
     1321 gggggcgccg gcaacgacgg cctggcgacc ggcggcggca cggactcggc cttcggggac
     1381 gccgccgacg attcgggcac cggcgacgac tcgatcgagg ccgggttcgg ggacgacacc
     1441 gtgcgcggca gcggcggcaa cgacctcctc gacggcggcg ttcgcttccc cttcttcccg
     1501 ttctctcccc catcgactga aagcgacgtg gacgacgtcc gcggcggcgc gggcaacgac
     1561 accgtcaggg gtggcggcgt cgggaccgat tcgctgctcg gaggggaggg cgacgactcc
     1621 ctgggcgacg gcgacatgcg ggagtccccc gcctcggcgc agagggtgga cgctgacgta
     1681 caggacggcg ggcccgggac ggactcggtg gactacggct tccgcgccga cgacgtggtc
     1741 ctgaccctgg gcgacgacgc ggcgaacgac ggcgaggccg ccgacgggtc gcggccgcct
     1801 gagaacgaca ccgtgcggaa cgtcgagaac gcgaagacgg ggtccggcga cgacgtgctg
     1861 accgggaccg ccgggccgaa ctccctcgag ggcggagagg gcggggactc gctggaaggc
     1921 gccggcggac ccgacgtcct cggcggccag gggggcggcg acacggccag cggcggcgcc
     1981 ggcgacgacc aggtcagcgg cggcgccggg gacgacacga ccagcggggg ctcggacgac
     2041 gacgagatat cgggcggcgg cgggaccgac acggtcaacg gcgacgacgg ccacgacgtc
     2101 ctgcacgacg gcgacgcgat cggcatgccc gacgccgacg tcctgaacgg gggcagcggc
     2161 cgcgacgtcg tgtcctcgta cgaggcccgg cgggccagcg tgaccgtgga cctgggcggc
     2221 gcggcggtga gcggcgtggc gggcgagaac gacgtgctca ccgggctcga gaacgcggcg
     2281 accggcctcg gcgacgacac gctcaccggg agctcgtcgg cgaaccacct cgccggggga
     2341 cgcggcgacg acacgatcag cgggggcgac gggagcgacg agctccaggg cgggttcggc
     2401 gccgacaccc tgacgggcgg tggggcgagc gacgcgctgg acgggggcga gggccccgac
     2461 acgctcaact ccggcgacgg cgggccggac cacgacggct gtggcgcgga cgtggacacc
     2521 gtcaacggcg acggcttcga cacggtcgat ggcgactgcg agaacctgac cggcggcgcg
     2581 acccgcagcg gtccgggccc gcagggcccg gccggaggca ccggtccggc cggaggcacc
     2641 ggcccggctg gaggcaccgg tcccgccggc actcagggcc cggcggggcc agccggaccg
     2701 gccggcccgc agggcacgcc cggaccgcag ggaccggccg gtcccgcggg cgcgagcggc
     2761 ggcaaggtcg cctgcaagct gaggggacgg aggatcacct gcaccttcac ccccaccaag
     2821 aggaagggcg cctccagggt cgtggtcgtg ctgcggcgcg gcggggtcaa ggtggcgcgc
     2881 ggcagcgcca cggtccgcgg cgcgacgcag gtccgcatga cggcgaagcg gcggccggcc
     2941 ccaggccgtt actcgatcgc ggtggtcgtc tacggcgacg gctcgacggt gcggctcgac
     3001 ggcagtacca cgctgcgctg actcagccgt agtcgtagaa gccggcgccc gacttcctgc
     3061 cgaggcgccc ggccgcgacc agctcgcgga cccgctcggg caccgtctcc ccgatcgact
     3121 ccccgatcgc cgccgccacg tcgaggccga cgtagtcgag cagcttcagc gggccggtcg
     3181 ggtgcccggc gccgagcttc atgcacgcgt cgacggcctc gggctcgatg ccctgctcct
     3241 cgaccagccg gacggccgcg aacaggtagg ggaacagcag gcggttgacc acgaagccag
     3301 gcgcgtcggg gacctcgatc ggggtcttgc cgagcgcctc gcagagcgcg cgcgtgcgcg
     3361 ctcgggtcgc gtcggcggcc gccggcggga acgcgagctc gaccagcgcc atccgctcga
     3421 ccgggttgaa gacgtgcagg gccgcgaagc gatcgggccg gccggcggcg cgcgccagtc
     3481 cctccaccga cagcgacgag gtggtggagg ccaggattgc gccgtcgtcc gtggcggcgt
     3541 cgagctcacg cagcaggtcg cccttcacgg ccgcgtcctc gacgaccgcc tcgaccacgt
     3601 aggtgcaccc gctcaggtcc gcgaggtcgg tcgtgacgcc cactccgcag cggatctcgt
     3661 cgcgtgcgcg cgcggccgac tccgggctgc gcgcccagac cacgactgcg ccgaggggcg
     3721 cggccaggcg cgccagaccg cacgcgatcg cgccagtccc cgcgatgccg acacgctcgc
     3781 tgacgtttgg ttgactcacc agtcaattct gtacagtaac cgtgcgcgga ccgtccaccg
     3841 gccgcgacgc caaacgacct tcggagggga tttccgcatg cagaacggaa ccgggcgcga
     3901 ggtagtcatc gtcgaggcgg tcaggacgcc gatcgggcgc ggccatccgg agaagggcta
     3961 ctacaaggac acccacccga acgcgctgct ggccaagtgc tacacggagg tcatcgagcg
     4021 cgccggcatc gagccgaccg aggtcgagga cgtgatcacc ggctgcgtgc agcagtatgg
     4081 cgagcagatg ttcaacgtcg ggcgcaacgc ctggctccag gcgaacctgc cggtggagac
     4141 tccggccacg acggtcgacc gccagtgcgg ctccgcgcag caggccgtga acttcggcgc
     4201 cgcgctcgtg gccgccggcg tgcacgacgc cgtgatcggc tcgggcgtgg agcacatggg
     4261 ccacatcccg atgggcgtcg gcttcacctg ggtcgaccag gtcggcagcc cgtggccgcc
     4321 cgagctgctc gagcactaca acctcgtgcc gcaggggatc tccgccgaga tgatcgccga
     4381 caagtgggag atcccgcggt cggagctcga cgagatcggc gtgcgctcgc accagaacgc
     4441 cgcgcgggcg accgaggagg gccgcttcga gcgcgagatc gtccccttcc aggtgaacgg
     4501 cgacacgtac gtgaccgacc agggcatccg gccggacacg aacctcgaga agctctccgg
     4561 gctgaaggtc gcgttcccgg acgccgcctc gcacgtcggc ggtgaggaga acctcaaggt
     4621 gaccgccggc aactcgtcgc agatctcgga cggcgccgcc gccatcctgc tgatgacccg
     4681 cgagaaggcg gatgccctcg ggctcaagcc gcgcgcgcgg atcctcgacc agaccacggt
     4741 cggcgtcgac ccggtgatca tgctcacggg cccgatccct gcgacgacga agctgctcga
     4801 gcgcaacaag atgaagatcg acgacatcga cctgttcgag gtgaacgagg cgttcgcgtc
     4861 agtcctcgcc gcgtggcgcc gcgagcacga gccggactgg gaccgcgtga acgtgaacgg
     4921 cggcgcgatc gcgctcgggc acccgctcgg gtcgaccggc gcgcgtctga tcaccacgct
     4981 gctgcacgag ctcgagcgct ccgacaagga ggtcggactc gtgacgatgt gctgcggcgg
     5041 cgggctcgga accggcacgc tgatccagcg catctagttc ggatggcggc tccccggggt
     5101 aaatccccgg ggtgacccct acgtttaccc agacggtcgg cgggcgctat cgcgtcgtcc
     5161 ggcgcctcgg cgcgggcggc atggccaccg tgttcctggc cgaggacgag cgcctcgggc
     5221 gccaggtcgc ggtcaagcgg ctgcatggcg agtcgaccga ggaggtcgcg cggcggttcc
     5281 ggcgcgaggc gcgcctgggc gccgcgttga accacccgaa cgtcgtcgcc gtctacgaca
     5341 tcacggccga cgacgagggc gtgctgatcg tcatggagta cgtggagggg cagacgctgc
     5401 gcgacgagat cggcgacggt ccgctgccgc cggagcgcgc gatcgcgatc ctgcgctgca
     5461 tcgcgaaggc cctggactac gcgcacgaga acggcgtggt ccaccgcgac gtgaagcccg
     5521 ccaacgtgct gattcgccgc gacggcgtgg tcaagctcgc cgacctcgga atcgcgacgg
     5581 cggccgagca gtcgaagatc acccgcagcg ggtcggtgct cggcacggcg gcctacatgg
     5641 cgccggagcg cctcgacggg cgcccgggcg gcccggcggt ggacgtctac gccctggcgg
     5701 cggtggcgtt cgagatgctc tgcgggcgaa aggcctacga gggcaacacg ccgctcgagg
     5761 tggcgcacaa ggtcgtgaac gagccgacgc cggacctgcg ccgctgcgtc gagagcgcgc
     5821 ccgagccgct ggcggaggcg ctcaagctcg gtctggcatg ggagccggac gcgcgcccgg
     5881 cgcgcgcggg cgacctggtg gcggcgatcg aggcggccct gccggaggcg gccgcgagcg
     5941 ccgcggtgga gccctcggcc gacgcgcccg cggcgcatgt gttcgccccg gaggccgagg
     6001 ctgcctccgc gtcggcggac ggcggcagtg aggaacaggg cacgcccgtg cgagttctcc
     6061 cgccggagcg cacgcggacc cggccggccc gcacggccgc cgccgcggcc ccggccgccc
     6121 ccgctccgac caccgccccc gcggcccaac ccgctcccac tccggacgtg ggccaagtta
     6181 ggggtcaaat acgaccaaaa gatggcccag ttggggagcg cggagccgaa acgagcgcgg
     6241 ctgggggctc ggctgggcgc ccggagcgag ggcgccggct cggcctcccg gtgatcgggg
     6301 cggccctggc aagcgtcgcg ctgctcgtga tcctgatcgt cgccctcgcg ggcggcgggt
     6361 cgggggatac ggagggcgat cgggtcgaca agggcgacac gaagggcgcg accgcggaga
     6421 agggctcgag cggcgacgag agctcgaacg gcgagaacgg ctccagcggt gcgcagggct
     6481 cgaacggcga gggctcgacc ggttccgagg gcggctccgg cggcagcgcc ccggccgaca
     6541 ccggcagcgg cggcggctcc accccggccg ccggcacggc cgccgcggcg gtcaacgact
     6601 tctaccagcg cgccgcgcgc ggcgactacg ccggcgcctg ggcgctcgcc accccgcgtg
     6661 cccaggagaa gctcggcggc ttcagctcgt tcagcgcggg ccagtcgacg ctcgacacga
     6721 tctcgttccc caagctccag ggcaaggaga gcggcgacac ggcgaccgtg accctgcaat
     6781 cgcgggcggt ccaccgggac cggatcgatc gctgcagggg gagcgtcgac ctcgttcgct
     6841 ccggtggcgc gtggaagctc gacgacttcc acatcgcgac ctgcgcgaag tccccgcgcc
     6901 cgtagcggcg aagccgccgc gccggggcgc agcccccgtc ccgtggtgtc ctgaccgggg
     6961 ctagactcct ccgtagaaga ggggtccaag gacgaaccac tcacccgacc agccgccggc
     7021 gggccgcagc tccggaggcg ccatgggcgc cgtctcacgg ctgctgtcga tcgccaccga
     7081 agccgagcgc cccgatgcgc ttgcgcgagc cctcgtccgc gaggcgcgcg ccgagttgcg
     7141 cgctccggcc gcggtggtgt tcgcgatcga ccagcgcagc ggccgcgcgg tggtggccgc
     7201 cgcggagccg cagaccccgc accgcgagtg gttcgccgtc gacgacgcct cgccgctggc
     7261 cgacgccctc gaggccggcg ccccggtggt gctgagcacg gcgcaggcgc gcgagctggc
     7321 cctggcgagc ggcctccagc cgcgtccgca ggcggcactg ctcgcgccaa tgccgcaccc
     7381 gggcggcgcg ctcgcgtacg cgctgatggt gggcgacgac cgcacgcgcg tcttcgaggc
     7441 ggacgagctc gaggccgccg gcgcgctggc cgaggccgcg cgggcgagca tggcccagcg
     7501 ccagctcgcc gagcgccacg gcaccgagcg cgcccgccag gcggcgatcg cgcgggccgc
     7561 caaggccctc aacgagagcc tcgacctcac ccgcgtcctg ccccgcatct gcgaagaggc
     7621 cgccgcgatc ctcgaggccg accgcgtgtc ggtgttccgc ggctcggccg gcaacccgct
     7681 gacgctcgag gccaccaacc agacgcccga ggccggtccc gccgcgcccg agccgggcgg
     7741 ggggatctcc gcgaaggcgc tcgacctcgg ccgcccgatc ctcaccaacg actacgcgcg
     7801 cgacggtgcc ccgaccgtgg gctcgccgtt cgcggcgatc cgcggcgcca tcgccgtccc
     7861 catgcgctgg gacggcgagc tgcgcggcgt cctctggacc ggctggacga gcgaccggct
     7921 gatgaccgac gacgatgccc ggctcctcga gagcttcgcc gagctggccg ccgtcgcgtg
     7981 ccgcaacgcc agcgtgcacg cggacctggc cgaggccgcc cgcaccgacg ggctcacggg
     8041 ctgcatgaac cacgccgccc tgcacgagac gctccgtcgc gagatgcagc gcgcgcggcg
     8101 caccggccgg ccgctgacag tcgtgctcgt ggatctcgac aacttcaagc aggtgaacga
     8161 gaaccagggc cacctggtcg gcgacgaggt gctgcgccgc gtcggccgcg ccctgcgcca
     8221 ggccgtgcgc ccgtacgact tcgtggcccg ctacggcggc gacgagttcg cgatcgtctc
     8281 ggtcgactcc gaggaggagg gcggcgccga gatcgcccgc cgcgcgatcc agcgcctgag
     8341 cgagtcggtg gccgagatcg ggcacgacaa ggcggccacc ttcgccacgg ccggcgtggc
     8401 cgagtggacc acggccctca gcccgagcga gctgctgcgg gtggccgatc gcgcgctcct
     8461 ctacggcaag cagcagggcg tgcgcggcaa ggcgatcccg gcatcctcgg tgccggagga
     8521 cttccggctc ggccgcggcg gcgcgcccga gcaggtggag gccacgccgc ccgcgcagct
     8581 ccccggctcg caggcgctcg acggcgagcc gctgcgtcgc cgcaaccgtc agctcaccct
     8641 ggcgaacgcg ctcggcgcgc gcctgtcgga gatgacggtg gtcgacgaga tcgtcgaggc
     8701 cgccgtggag gagctgcacc gcgcgttcgg ctacttcacc tgcgccgtcg tgcgccgctc
     8761 cgaggacggc gagtcgatcg acctgctcgc gggccgcggc gccgcctacc aggaggtcac
     8821 cgcgtggcgc caaccgatcg ccgccggcgt gatcggccgc tgcctgcgcg agcgccgcac
     8881 gatcgtggtc aacgacgtga gccgcgactc cgactacttc gcgaccgccg cgaccgaggc
     8941 ggtgcgctcg gagctcgtct cgccgatctg ggtggggccg gagctgtggg gcgcgctcga
     9001 cgtgcaggag gtcaacgagg gcgcgttcga cgaggccgac gcgcggctga tcgagaccgt
     9061 caccgaccag ctcggcgccg cgctgcgctc ggcgatcctc tacgagcagc tcgagcgcgc
     9121 gtacctcggc accgccgagg cgctggccac ggcgctcgag gccaaggacg cccacagcgc
     9181 cagccacgcg cgctcgatcg tgagcaacgc ggaggcggtg gcacggctgc tcggcctcgg
     9241 cggccaggac ctgcgcgacg tccgatacgg cgccgcgttc cacgacatcg gcaagatcgc
     9301 ggtgccggag ggcatcctcg gcaagcccgg cccgctgagc gacagcgagc gcgcgcaggt
     9361 cgagcgccac gtgctgatcg gggagcagat cctcgccccg gtcgagttcc tctcgcgcgt
     9421 gcgcacgatc gtccgccacg ggcacgagcg ctgggacggc accggctacc cggaccggct
     9481 cgccggcgac gagatcccgc tcggctcgcg gatcgtgctg gcctgcgacg ccctcgacgc
     9541 gatgaccacc gaccgcccct accgggaggc cctcccgcgc aatgcggccc gctccgagct
     9601 gcgcctgcac gccggcagcc agttcgaccc gcgcgtggtc gacgcgctgc tcgaggtgct
     9661 cgaccgccgc gaggcgccga cgctcgagtc ggccaagtag cgccggcctc ccctgaatcg
     9721 gggattgacc gcgcggagat actcgtcgca tggccgagtc gaagcaggcc atcgcctacg
     9781 aggcggcgac gcgcgccctg agcgaccagc gcgccagcct cgacaacctc cgaagccgcg
     9841 ccgcgtcgct ggtggccgcg gcggcgatcg tgacgtcgtt cctcggcgcc gcggccctgg
     9901 ccgacactcg cctgacgcgc gcgacgggca ccgcccagtt cgtgccggac cggtcgctcc
     9961 agccggccga ggccgtcgcc ctcggtgcct tcctactcgt gctcttcctc tgcctcctga
    10021 tcctgcgccc gcgcgggaag tggaagttcg tgctcggtgc caagtggatc ctggaacgcg
    10081 acgaaaagga cgaggacgcg ctgatgaagc tgctcgccga gtggtcggag acttactgga
    10141 agcggaacaa caagtggatc aagcgacgca tggacatgtt ccaggtcggg gtgttgctcc
    10201 tgggcgtgga gtccgttgcg tgggttgtcg atctggcgtg aggaggaagc gatgacgtgg
    10261 accgagcgcg agccggacgg tccggccggg gagtccgacg acgacggtcc tgggctcgag
    10321 gagaccagcg ctctcgagga ggcggacagc gacccggcgc cggcggacga cgacggcccc
    10381 ggcctgaccg aggactacga ccaggaagga ccggcctgac cgaggactac ctgtagcgcc
    10441 tgagacggcg cggccgccgt gggcgaggat cgtctgtaga gtccgcgact cgtggacctc
    10501 acgctgtcgc cacaggagca ggccttccgc gacgagctgc gcgcgtggct ggaggagaac
    10561 caccccggcg aggagcccga gggcgacgag agcgcgggct tcgagttccg ccgcgactgg
    10621 cagcgcaagc tcgccgaggc gcgctgggcc ggtctgtcct ggcccgagga gtacggcggc
    10681 cgcggcgcga cgctgatcga gcaggcgatc ttcaacgagg agatcgtgcg cgcgaagtcg
    10741 ccccagatgg cgaacgtgct ggggctcgtg atgggcggac cggtggtgat cgcccacggc
    10801 accgacgagc agaagcagcg ctacctcaag ccgatcctca cggccgacga gatctggtgc
    10861 cagggcttct cggagcccga gtccggctcc gacctcgcct cgctcaagac caaggcggtc
    10921 aagtcgaacg gcgagtggat agtgaccggc cagaaggtct ggaccacctt cgcccacgag
    10981 gcgaagtggt gcatgctcgt cgcgcgcacc gacatggacg ccccgaagca caaggggctc
    11041 acctacttcc tgatggacat ggagcaggag gcggtgcagg tgcgcccgct gcgccagata
    11101 accggcgagg ccgagttcaa cgagctcttc atcgaggagg cccggatccc ggacgagaac
    11161 atcgtcggcg gcgagggcaa cggctggatg gtcgcgatca ccacgctcat gcacgagcgc
    11221 gccggcctcg ccctgggcct ccaggtgcag ctcaagatcg tgatcggcga gctgatggag
    11281 gagatccgcg agcgcgggct cgaggacgac ccgatcatcc gccagcggct cgcgcagatc
    11341 tacatcgagt ccgagtgcgt gcggctgaac gcgtcgcgcg gcctcacgcg cacgatgaag
    11401 accgggatcc ccgggcccga gggctcgctc accaagtggc agtggtcgga cgcgaaccag
    11461 gcgctgaccg agctcgcgat ggacgtgcgc gggccggacg cgcccgtgat cgacggcgac
    11521 tggacctacc gcttcctgcg cgcccgcgcg aactcgatcg agggcggcac caccgagatc
    11581 ctgcgcaaca tcgtggccga gcgcgtgctc gggctgccca agctgaggtg agcgcgtgaa
    11641 cttcgactac tccgacgacc agcaggcgat caagtcgacc gcgcgcgact tcctcgcgag
    11701 ccggttcaag ctcgagaagg tgcgcgagct ggcgatcgcg ggcgagtacg acgacgggct
    11761 ctggggccag atcgccgacc tcggctggcc ggggatcttc atcgacgagg cccacgaggg
    11821 ccaggacctc ggcgtgatcg agctggcgat cctccaggag gagctcggct acgcctgcgc
    11881 gcccacgcca ttcttctcca acgcggcggc cggcctgctc gtgaagcacg gcgggtcgag
    11941 cgagcagcac gagcgcctgc ttccgggcgt ggcgtccggc gaggcgcgcg gcacggtggg
    12001 gatcgtgaag gacggcgcgg cgccgctggt gcccgacgcg gacaccgcgt cgttcgtagt
    12061 gctgctcgac aacggcacgg ggcagatcgt cgagaccgcc tcggctcaga tcgagaaggt
    12121 ggacacgatc gactccaccc gccgcttctt ccgcgtgtcc gccgatggcg gcgactcgct
    12181 cgagggcgat ctcagcgccg ggttccagcg catcgaggtg gccctggcgg ccgagctcgt
    12241 cgggctggcg cagcgcgcac tcgagatgtc ggtcgagtac gcgaaggacc gcaagcagtt
    12301 cgaccgcccg atcggcgcct accaggccgt ctcgcacaag tgcgcgcaga tgatgctgga
    12361 gaccgagggc gcgcgggcgg ccacgctgta cgcggcctgg gcggccgacc acgagccgga
    12421 gtcgctcccg ctcggcgcgt cgatggccaa ggcctacgcc gccgacgccg gctggcgggt
    12481 caccgccgcc gcgctccagg tgcacggcgg catcggcttc acctgggagc acgacctgca
    12541 ctggttcctc aagcgcgccc gcacaaacgg cgcgctcttc ggctccgcgc gcgagcaccg
    12601 cgaccgcgtg gccgagcgga tcgggctgtc gcgctcggcc gcagcggcgt agcgccgcag
    12661 ggccgtcgcg cgggcgtacc cttcgcgcct tgagcccctt cgatcgtctg cgccgcctca
    12721 tgcccggcgg cggcgagccc gacctcgacg ccgagctcag gtggtggctg gacgagtggg
    12781 agccgatcgt gcgcgacggt ggcttcaacc ccggcgacgt gcccgcgttc ctgccggagg
    12841 gcgagccggt cgccgacacc ttcgagggcc gccgctggca gatcgcgcgc gcggaggtgg
    12901 cgcgcgtcct gacggaggcc gggatcgagg acgaggactt cttcgagggg aaggtcgtgg
    12961 tcgacatcgg ctccggcccg ctcggcttcc ccgacgcctg ccccgccaag ctcgccatag
    13021 ccgtcgagcc gctcgccgac cgctaccgcg ccgccggcct gctgctgggt ggcgagaaca
    13081 cggtctacct cgcgacgggc gccgaggcga ttccgctgct gagcgactcg accgacgtgg
    13141 tgctcacgcg caacagcctc gaccacgttt cgaacccgac cgcggtcgtc aacgagatcc
    13201 tcaggatcct gagacccggc ggcacgctga tcctcagcgt cgacatcggc cacccggcca
    13261 ccgccaccga gccgcacagc ttcacgcgcg gggagatccg gatgctgctc aacccgctgg
    13321 atatcgtgag cgagcggacg ctcgacgagg cgcacggcgg aggcggcggg agtcgcatcg
    13381 tggtcgtggc tcgccgccgc tgagcgcaac cctccgcgtc tctccgcgtt ctctcttccg
    13441 atgcccagtc gcctgatccc actcgcgctc ctgctcgtcg ccctcctcgc gcccgcgcgg
    13501 gccagcgcgc agcagcgcca ggtcccgcag ggctacttcg gcgcggtcgc cgacggggtg
    13561 ctcaccgacg aggacccgca acgcttcgag aacgagctcc cgcggatggt gggagccggc
    13621 gtcgagtcgc tgcggatggt gttcccgtgg cgcgacgcgc agccgtacaa gacccgcgcc
    13681 gagatcccgt ccgaccagca ggcgttcttc cgcgacgagg gcggggtccc gaccgactgg
    13741 cggctgctcg acggctacgt cgagcacgcg gcccgccagg gcctcacgat cctgccggtc
    13801 gttctctacg cgccccagtg ggccgccaag tacgggaact ccgacgcctc gccgccgcgc
    13861 gggcacacgg actacgcgcg cttcgtcgtg gcggtggcca agcgctacgg ccgctccgga
    13921 accttctgga ccgagcgccc ggacgtgccg cggctgccga tcgactggct ccagatctgg
    13981 aacgagccgc acttcaagga gtactggtcc gaccagccct gggaggcgga ctacgcgcgc
    14041 ctcctgcgcg gcacctaccg ggccgtgaag aaggaggtgc cgagcgcgaa gatcctcgtg
    14101 gccggcatgg cgaacaagag ctggacgtac atctcgaagc tctacgccca gaagacgcgc
    14161 ggctacttcg atgggatcgc gatgcacccg ttcacgaact cgatcgacgg cgtggtcgag
    14221 atcatcgagc gcgggcgccg cgtgatggcc cgcaacggcg acggcaagct gccgctgatg
    14281 atcaccgaga tgagctggac ctccgcgaag ggcaaggccg gctacacgta cggaaacgag
    14341 cgcaccgagg cgggccaggc gaaggcgctg cgccagggcc tccagcggat ggcgaaggac
    14401 cggcgggcgc tgcgcatcct gcacgtgtac tggtacacgt ggatgacccg cgacaagcac
    14461 gagaacatcc cgttcgacta cgccggcgtg atccgcttcg agtccgacgg caccacgacc
    14521 gagaagccgg cgttcgcggc gtaccgctcg acggcgatgg cgctcgaggg ctgcgctggc
    14581 aagcccagcg gcgcccgcag ctgcaccccg cggcgctagc cgccgaggcg cgcctcgatc
    14641 agcgcgccgc tcgctcctcc tcgagccggg cctcggtgac cgggtcgggc ttgtcctcgt
    14701 ccttcagccg cggcacgatc gccatcagga cggcggccgt cccgatcgcg tggatgccga
    14761 gtgccagcgg gaggaagatc cagttgtcgg tgaacagcgc ggcgatcagc ccgacgacga
    14821 gcgccgtggc cgcggtgaag atcacgagca gtcgtggcga tcccgatgac atggccacgg
    14881 gctacccgcg gtgggcgctc ccgaatcagt cgacgcgcgg gctacaggag cctgccgccc
    14941 acgaactggg tcacgccggc gacgcgctgc tgcacgttgc cgtagccgcg cgcctccagt
    15001 gcccgcacca cctcgccgag ctcgaacatg cgcatcccgc tgcggcgccc tgccatcgag
    15061 gcccacggcc gcatcagcgc cgtgcgcccc tgcgccgagg tgaacagcgc gatgcgcccg
    15121 cccggcgtga gcacctcggt catgcggtcg agcgcccgca tcgggtcctc gaacaggtgc
    15181 agggcggcga agcagcacac ggcgtcgaag ctcgactcga cgaacggcag ctcgacggcg
    15241 tcgccgcgca cgaaggcggt ccggtcgagc agcgcggcgg cgtcggtgtc ggtgacggcg
    15301 cgcgcgagca tcgtctcgga gacgtcgatg ccgacggcca ggccgtccgg cccgaccgtg
    15361 cgcgcgaact cgcgcgtgaa gttgccggtg ccgcaggcca cgtcgagcac gccgtcgccc
    15421 ggcgagaggc cgagcaggag ccgcgcgatg cggtgctcgt cctgcatccc cggtccgagc
    15481 acgcccttcg ccacgcgccc gagcgccggg cgccaccagc gctcgtagat cgccggtacg
    15541 acgcggctga ccatcaggtc ctgggtgacc ccggtcgatc gcggtgcggc atcgcccacc
    15601 aggtcgaggt agccctcctc gcgcagccgc ccgtcctcgc gcgcccacgg cgcgagcagc
    15661 ggcagcgccc ggtcgagcgc cggcggcagt cgcgtgaagg cgcggcccac ggaacgccgc
    15721 ttacgcgggt tcgtcgccgc gtacgctggg cagcgaccgc tcgccctcgc gggcgggtcc
    15781 gcgctgctcg ccgctgaacg cgatgccgtt caggcgctcg atctgcatct cgccctcgac
    15841 ccgcgccggg tgcccctcgg gcagcccgcc gatcaggttc cggatccggt tgcggatgtg
    15901 cggcgcgaag tggggggtgg aggggcccat gagctcgcgc acgtcctcga cggtcggctc
    15961 gcgcccgacg cgatcgggct tctcgaactc gctcacgccc ggaaagctac cgggagccgg
    16021 ctaggagccc gagtcgggcg agcggtccac cgggatgagc tggagcagct cgtccacgcg
    16081 cgcctgcacg ccggtggcca cggcgtcctc gttgccgagg acccagacgc ggttgtacag
    16141 gccctggctc ggatcgccgt tctcgaagcc gggctgcacg tcgagcaggt agttctcgag
    16201 cggcttgggc agcgcgtcgc cgcggtctgt cacgagcaga ggcgcgtaca cgccgttgct
    16261 gccgagcgcg gcggccgccg cggcgtcgag cgggcgcgag gcgttcgcga gcgcgtagtt
    16321 gcggcccggc acgaccgcgc cccagccgaa gcccttcgag ttgaaggcgg tgaactcgac
    16381 ggcgctctcg accgggttgg tcgccttctc gccgatgcgc acgaccgtgc cgagcgcgtc
    16441 gagctgcttc gcggtagccg gagacacgat cgacggcggg ccgagcacgt agatgttggg
    16501 cttcgagtgc tgctggatcg cctggcgcgt ggcctgcggg acctcgttct tcttcacgaa
    16561 caggaccggc gtgccggagc gggcggccca cgaggcggcg ggcattgcgt aggcggggtc
    16621 ctcgcccgag acgacgagaa cgtcgggcgt cggcttgccg gccgcggtgg tctggaagcg
    16681 gtcgatcgcg gccgcgatcg cgtaggggtc ggaacccttt atcgcgccgc tcttgaagcc
    16741 gtccggcgcg ggcggcttgt cgccgaccag gatcacctgc gcgcccttgg cgagcgtctg
    16801 gccgcgcggc ttgaggcgcg ccagcgcgtc cttcgtgacg gctggcaggt cgccgccgtc
    16861 gctca
//
LOCUS       DASVPL010000160         8350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_256437, whole genome shotgun sequence.
ACCESSION   DASVPL010000160 DASVPL010000000
VERSION     DASVPL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256437"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VF517_08210"
     CDS             <1..1069
                     /locus_tag="VF517_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX8052962.1"
                     /translation="MHDVTFAPKDGTTIPHLVFFTFQATFCIVTTALVSGAVVERMRF
                     GAYLIFAGLWSILVYAVLAHWAFGGGWLIDNGTLDFAGGVPVEMGSGFSALAAALVVG
                     ARKDYGRQALLPHNSIYVLLGAGLLWFGWFGFNGGSGFTTGQNSVLAFTNTLLCPAAT
                     LVVWFGLDALRGRKATAIGAATAIIVGCVLITPAGGFISPGWAMALGAAGAFPCYALI
                     MFRPRTRVDETLDVLAAHGIAGFTGILFIGFFAQQNWNGLSDGLLYGNGDQLKWQALA
                     ALATPIYAFVMTFGLLKLIGLVIPLRATERDEALGMDVTQHGEEAYPSGEGAILVLPP
                     DGDGVRGTERAGVPVGDPGLA"
     gene            1135..1548
                     /locus_tag="VF517_08215"
     CDS             1135..1548
                     /locus_tag="VF517_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052963.1"
                     /translation="MAVGWRRIGDLRAIGADREALKAELAEAYPDAKPRAIPVWAGVL
                     LRFAFEMRVGDLVVHPCKGDSTLSFGRVAGGYRWEPDAPPDNRHRRAVDWLEVGVPRM
                     VFSESARHELGSAITLFRVRRNADELRAVVEGAVA"
     gene            1751..2281
                     /locus_tag="VF517_08220"
     CDS             1751..2281
                     /locus_tag="VF517_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052964.1"
                     /translation="MLVASGAMYALGVFTGISDGLSLRWSAQTANLRAKGAPKEDKHP
                     PVDPVPGPGGGRGPSDGGASQLDEGDHAGSGGRGRRHEPEPEQTRRPDKKDGRGRDDE
                     SEDNQLPEDDEGGDAAPPGEDKGEGKGGGSDGDAGDGDDTDPGGPGPIPTVPAVPVTP
                     PGEDDLPPPPPRRLLA"
     gene            complement(2278..3051)
                     /locus_tag="VF517_08225"
     CDS             complement(2278..3051)
                     /locus_tag="VF517_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052965.1"
                     /translation="MTKQWNDHPQELEVKTGVVKDLSESAATAISNARFLAANALPQA
                     AAQRVACARGAQRSQAGRERCIEEKRDKAEAVARTNMETVTGWRRTAAALEARVSAYF
                     EDEEGKWRTYSLAVERFLRLSTTECGQARDDNLRRLLAYLGESAPLDPTESGAWGPLF
                     KLTSAQCRSGAARGAKEGYRKANGDLSNKLLQKRGDLLDSLRDADSDAFSSGLGDFVS
                     DAGVPIVVGIVLAGLLLAAYPFAAWWRRRRRRRRSRAAL"
     gene            3028..3270
                     /locus_tag="VF517_08230"
     CDS             3028..3270
                     /locus_tag="VF517_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052966.1"
                     /translation="MVVPLLGHRWYEHGGRDYGEDEPGRFDRLPGELQRCGNDLFALC
                     LQARELFVREAVLQSPTVLELGGDEMASAILRMIRL"
     gene            complement(3341..4225)
                     /locus_tag="VF517_08235"
     CDS             complement(3341..4225)
                     /locus_tag="VF517_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052967.1"
                     /translation="MAAVTAIHFTDPGCPWAYSARPAHARLAWRFGDQIEWRLVMIGL
                     SETSQAYVDRGYTPALNTAGWRVFERRFGMPFALQLKERVAGTSRACRAVVAARAIDP
                     ALGEAALQALQLMHFASMGLLDEDDDLRAALARVPGLDAGAVVDSIESPHVVADYAAD
                     KTLARSADGSPTHVQGRFATTDGPVRFTAPSVIFEHAEGRRLEVGGFQPFEAYDTALA
                     NLDTSLERRPPPEDPVEALAAFPLGLTTAEVAAVMRPSDLVDADIPAAQDKLAWLAGD
                     GKVTCEPVGGDALWRLAG"
     gene            4353..4523
                     /locus_tag="VF517_08240"
     CDS             4353..4523
                     /locus_tag="VF517_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052968.1"
                     /translation="MIGRWGDDTALVRTDEGVTFEVRVPEALREGFDVGAELRIDPAS
                     GGILGWGPVGVR"
     gene            4584..6323
                     /locus_tag="VF517_08245"
     CDS             4584..6323
                     /locus_tag="VF517_08245"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX8052969.1"
                     /translation="MTISTQTLRVALAQIDAVVGDIDGNAAKIADQIARAREQGAQLV
                     LLPELALSGYPPEDLLLKTHFVDAAGAALRELATGVHGIVALVGFPERAGDVYNAAAV
                     LADGEIAAVYRKMLLPNYGVFDEQRYFQSGSEPALIELNGIAIGVTICEDIWTPGPPA
                     ATQALAGAQVIVNLSSSPYHAGKPLERERMLIQRARDNLAAIVLCNLVGGQDELVFDG
                     HSVAIDQNGTVLARAPQFEESLTLCSIETGPVASARLRDARHRSAVRAAGEPSVPVIA
                     RLTVEPRDTPAPDDAPVAEPLEPVEEIYAALRTGLRDYVTKNGFEGVVLGLSGGIDSA
                     LVALIAVDALGADRVTCVSMPSPYSSEATRSDARAIAANLGTGFVELTIEELMEGYES
                     VLAEQFAGREPDIAEENLQARIRGNIVMALSNKFGWLVLTTGNKSELSVGYATLYGDM
                     AGGFAVLKDVFKGWVYRLVEWRNEREGRELVPNTVLERPPSAELRHEQRDDQSLPPYE
                     VLDAILLGYVEGDLDADVLVAHGLPREDVERVIGLVDRAEYKRRQAPPGIRISTRAFG
                     RDRRLPITNRFGR"
     gene            6407..7375
                     /locus_tag="VF517_08250"
     CDS             6407..7375
                     /locus_tag="VF517_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX8052970.1"
                     /translation="MKLHANARTCPNSRRLLVDRILRESWSVTAAAEAAGVSERTAYR
                     WLARFRQEGAPGLLDRSSRPQRVHATPARLVEAIERLRRLRMTAAQITEILGVPLSTV
                     SLWLKRLGLGKRSRLEPPEPPNRYERRHPGELVHVDIKQLGRISPKGAGHRVVGHRAS
                     QSNRRVGARQRRTTRFEYVHVMVDDHTRLAYAEVLATLTSRCAVGFLRRGVAWFAHRG
                     VRVGAVMTDNGSSYTAHAHRRALETLGLRHLRTRPYRPRTNGKAERFIQTLLNEWAYE
                     RVFGSSAERAAALPLFLERYNYRRPHGSLGHKPPASRLTNVVGNYS"
     gene            complement(7372..7809)
                     /locus_tag="VF517_08255"
     CDS             complement(7372..7809)
                     /locus_tag="VF517_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEX8052971.1"
                     /translation="MNTEPSLPVEPRETLSGILEFEHLEVGQERAVGRFTVASRHKQP
                     FGLVHGGTYAALAEGLVSEATAQAVVPTGMLAQGMSHNLNFLRPVFEGSVEAVGTRRH
                     RGRTSWVWDVDMTDDRGRLCATSRVTVAVRPMPDEMKARLGIA"
     gene            7837..>8350
                     /locus_tag="VF517_08260"
     CDS             7837..>8350
                     /locus_tag="VF517_08260"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8052972.1"
                     /translation="MPTFDQLPAEQRAIIELVVQRGRTYEQLADALQITPERVRELAH
                     DALVELSPRSAARVDADRRTQIADYVLNQQSSAEATATRTHLKRSEAGRMWVSSLLDS
                     LEHMYEDGNAPDVPDAEAEEERPRRRERERERERERPRERERTRDRERDRDRDHERGA
                     AARERPRRDEP"
BASE COUNT         1201 a         2978 c         2977 g         1194 t
ORIGIN      
        1 catgcacgac gtcacgttcg cgccgaagga cggcacgacg atcccgcacc tcgtcttctt
       61 cacgttccag gcgacgttct gcatcgtcac caccgctctg gtgtcgggcg cggtcgtcga
      121 gcgcatgcgc ttcggcgcct acctgatctt cgcggggctg tggtcgatcc tcgtctacgc
      181 ggtgctcgcg cactgggcgt tcggcggcgg gtggctgatc gacaacggga cgctcgactt
      241 cgccggcggc gtcccggtcg agatgggctc ggggttctcg gcgctggcgg cggcgctcgt
      301 ggtgggagcc agaaaggact acgggcgcca ggcgctgctc ccgcacaact cgatctacgt
      361 gctgctcggc gcgggcctgc tgtggttcgg ctggttcggc ttcaacggcg gcagcgggtt
      421 caccaccggc cagaacagcg tcctcgcgtt caccaacacg ctgctgtgcc ccgccgccac
      481 cctcgtggtg tggttcgggc tcgacgctct ccgcggccgc aaggccacgg ccatcggcgc
      541 cgccaccgcg atcatcgtcg gctgcgtgct gatcaccccc gccggcggct tcatcagccc
      601 gggctgggcc atggcgctgg gcgcggcggg cgcattcccg tgctacgcgc tgatcatgtt
      661 ccgcccccgc acccgggtcg acgagacgct cgacgtgctc gccgcccacg gaatcgccgg
      721 tttcaccgga atcctgttca tcgggttctt cgcccagcag aactggaacg gcctgtccga
      781 cggcctgctg tacggcaatg gcgaccagct caagtggcag gcgctggccg ccctggccac
      841 cccgatctac gcgttcgtga tgacgttcgg cctgctcaag ctgatcgggc tggtgatccc
      901 gctgcgcgcg accgagcgcg acgaggcact cggcatggac gtcacgcagc acggcgagga
      961 ggcatacccc tccggcgagg gcgcgatcct cgtccttccg ccggacgggg acggcgtgcg
     1021 cggtaccgag cgggcgggcg tgccggtggg cgatcccggc ctagcctgat cggcctgacc
     1081 gtctggggca tccacaacga caagccggcg tacgacttcg tcgaggacgg gcacgtggcc
     1141 gtcggctggc ggcggatcgg cgacctgcgc gcgatcggtg cggaccggga ggcgctgaag
     1201 gccgagctgg ccgaggcgta tcccgacgcc aagccgcggg cgatcccggt gtgggccggc
     1261 gtgctgctgc ggttcgcgtt cgagatgcgg gtcggcgacc tcgtcgtcca cccctgcaag
     1321 ggcgactcga cgctgagctt cggccgggtg gcgggcggct accgctggga gccggacgcg
     1381 ccgccggaca atcgccaccg ccgcgcggtc gactggctcg aggtgggcgt gccgcggatg
     1441 gtgttctcgg agtccgcccg ccacgagctc ggctcggcga tcacgctctt tcgcgtgcgc
     1501 cgcaacgcgg acgagctgcg ggcggtcgtg gaaggtgcgg tggcctgacc cggccggggg
     1561 gagccgccca cgctcctggg cccacctggc ccacgatccg catctgcgtg cggcgccgta
     1621 gatacctgct agtggggagt gtcaaggcgc caacccactg ctacgctcgc cgcgagggac
     1681 tgccgtccgg tttttgaggt acatccgccg tcataagctc gtcgtatgcc ttgcggcgag
     1741 cctcgcgatc gtgctcgtcg cgagcggcgc aatgtatgcg ctcggcgtct tcaccggaat
     1801 ctctgacggc ctatcgctca ggtggagcgc gcagacagcg aacctccgcg ccaagggtgc
     1861 cccgaaggag gacaagcatc cgcccgttga ccccgtgccg ggccccggcg gtggccgcgg
     1921 accatcggac ggcggcgcct cccagctgga tgagggcgac catgcgggat cgggcggtcg
     1981 cgggaggcga cacgagcccg aacccgagca gacccggaga ccggacaaga aggatgggcg
     2041 cggtcgtgac gatgagtcgg aggacaacca gctaccggag gacgacgagg gcggggacgc
     2101 ggccccgcca ggcgaggaca agggcgaggg caagggcgga ggaagcgacg gcgacgccgg
     2161 tgatggcgat gacaccgacc ctggcgggcc cgggccgatt ccaaccgttc ccgcggtgcc
     2221 ggtgactccc ccgggcgagg acgaccttcc gccgccgccg ccgcgccgtc tcctggccta
     2281 gagcgcagct cgcgaccgac gcctacggcg ccgccgacgc caccaggcgg caaacggata
     2341 ggcggcaagg agaaggccag ccaggacgat gccgacgacg atcggcactc cggcatccga
     2401 gacaaagtcc cccaggccgc tgctgaacgc gtcgctgtct gcgtcgcgca gcgaatcgag
     2461 gaggtcaccc cgcttctgga gcagcttgtt gctgagatcg ccgttcgcct tccggtaccc
     2521 ctccttggca ccacgtgcgg ccccgctcct gcactgcgcc gaggtgagct tgaacagggg
     2581 cccccaggct cccgattccg tcggatcgag cggggccgac tcaccgaggt aggccagcag
     2641 cctgcgaagg ttgtcgtcgc gggcttgacc gcactccgtg gtcgacaatc ggaggaaccg
     2701 ctctaccgca agcgagtagg tgcgccactt cccctcctcg tcctcgaagt acgccgacac
     2761 gcgagcctcg agcgccgcgg cagtgcgccg ccagccggtg accgtttcca tgttggtcct
     2821 cgcgacggcc tccgccttgt cgcgcttctc ctcgatgcag cgttctcgcc ccgcctgcga
     2881 tctctgcgcg ccgcgcgcgc aggcgacccg ctgggcagca gcttgtggca gagcattcgc
     2941 tgccagaaat cgggcattcg agattgcggt cgcggcggat tcgctcagat ccttgacgac
     3001 gcctgtcttg acctcgagct cctgcggatg gtcgttccac tgcttggtca ccgctggtat
     3061 gagcacggcg gtcgtgatta cggcgaggat gaacccggcc ggtttgaccg cctgccgggc
     3121 gagctgcagc gatgtggcaa cgacctcttc gccctctgcc tccaggcgcg tgagctcttc
     3181 gtccgagagg cggtgctcca gtcgccgacc gtccttgagc tcggtggtga cgagatggcc
     3241 tccgccatcc tgaggatgat ccgcttgtga gatttcttgc ccccggtcag aaaagttaac
     3301 tgactcaaaa cgcgcgcagc gcaggggtcc ggcgcagtcg ctagccggcg agccgccaca
     3361 acgcgtcgcc gccgaccggc tcgcacgtga ccttgccgtc gccggcgagc caggcgagct
     3421 tgtcctgcgc ggccggtatg tccgcgtcca ccaggtcgga gggacgcatc acggccgcga
     3481 cctcggccgt ggtgagcccg agcgggaatg cggcaagcgc ctcgaccggg tcctccggcg
     3541 gcggtcggcg ctcgagcgac gtgtcgaggt tcgcgagcgc cgtgtcgtat gcctcgaacg
     3601 gctggaaccc cccgacctcg aggcggcgcc cttcggcgtg ctcgaagatc accgacggcg
     3661 ccgtgaagcg gacgggcccg tcggtggtcg cgaagcgtcc ctgcacgtgg gtcggcgagc
     3721 cgtcggccga gcgcgcgagc gtcttgtcag ccgcgtagtc ggccaccacg tgcggactct
     3781 cgatgctgtc aaccacggcg ccggcgtcga ggccgggcac gcgggcgagg gcggcgcgca
     3841 ggtcgtcgtc ctcgtcgagc aggcccatcg acgcgaagtg catgagctgg agcgcctgga
     3901 gcgcagcctc gccgagcgcg gggtcgatcg cccgcgccgc cacgacggcg cggcacgccc
     3961 gcgaggtgcc cgccacgcgc tccttcagct ggagcgcgaa cggcatcccg aagcggcgct
     4021 cgaacacgcg ccagcccgcc gtgttgagcg ccggcgtgta gccacggtcc acgtacgcct
     4081 gcgaggtctc gctgaggccg atcatcacca gccgccactc gatctggtcg ccgaagcgcc
     4141 aggcgaggcg ggcgtgtgcg ggccgcgccg agtacgccca cgggcagccc gggtcggtga
     4201 agtggatcgc ggtgacggcg gccacagagt cagcctaggg cacgagcagc gcccggttgt
     4261 cgcaccgcca gaccccgtgc ttaactcggc tgacattgag cgctagatct tccgtatcgg
     4321 gtcgcttcgc ggaaccgtcg cggcacgcgg tggtgatcgg ccgctggggc gacgacaccg
     4381 ccctagtgcg aaccgacgag ggggtcacgt tcgaggtgcg cgtgcccgag gcgctgcgcg
     4441 aaggcttcga cgtcggcgcc gagttgcgga tcgatcccgc cagcggcggg atcctgggct
     4501 ggggcccggt cggcgttcgc tgaccctggg cggggccgtg caggcccgct gcgagcctgg
     4561 cacccggcgg catactgggc gcgatgacga tttcgacgca gaccctgcgc gtggcgctgg
     4621 ctcagatcga cgcggtcgtc ggcgacatcg acggcaacgc cgcgaagatc gccgaccaga
     4681 tcgcccgcgc gcgcgagcag ggcgcgcagc tcgtgctgct tcccgagctg gcgctgagcg
     4741 gctacccgcc cgaggacctg ctgctgaaga cccacttcgt cgatgccgcg ggagccgccc
     4801 tgcgtgagct ggccaccggc gtgcacggca tcgtcgccct ggtcggcttc cccgagcgcg
     4861 ccggcgacgt ctacaacgcg gcggcggtcc tggccgacgg cgagatcgcg gccgtctacc
     4921 gcaagatgct cctcccgaac tacggcgtct tcgacgagca gcgctacttc cagtcgggga
     4981 gcgagcccgc gctgatcgag ctgaatggga tcgcgatcgg ggtgaccatc tgcgaggaca
     5041 tctggacgcc cggcccgccc gcggcgacgc aggcgctggc gggggcccag gtcatcgtga
     5101 acctgtcctc ctcgccctac cacgccggca agccgctcga gcgcgagcgg atgctgatcc
     5161 agcgcgcacg cgacaacctc gccgcgatcg tgctctgcaa cctcgtcggc ggccaggacg
     5221 agctggtctt cgacgggcac agcgtggcga tcgaccagaa cggcaccgtg ctggcgcggg
     5281 ccccgcagtt cgaggagtcg ctgacgctct gctcgatcga gaccgggccg gtggcctccg
     5341 cgcgactgcg cgacgcccgc caccgctccg ccgtgcgtgc ggcgggcgag ccgagcgtgc
     5401 cggtgatcgc gcgcctgacc gtcgagccgc gggacacgcc cgcgccggac gacgcgccgg
     5461 tggccgagcc actcgagccc gtggaggaga tctacgccgc gctcaggacc gggctgcgcg
     5521 actacgtgac caagaacggc ttcgagggcg tggtgctggg cctctcgggc gggatcgact
     5581 cggcgctcgt ggcgctgatc gccgtcgacg cgctcggggc ggaccgggtg acctgcgtct
     5641 cgatgccctc cccctactcc tcggaggcca ctcgctcaga cgcccgggcg atcgccgcca
     5701 acctcggcac cggcttcgtc gagctgacca tcgaggagct gatggagggc tacgagtcgg
     5761 tgctcgccga gcagttcgca ggtcgcgagc cggacatcgc cgaggagaac ctccaggcgc
     5821 ggatccgggg caacatcgtg atggccctgt cgaacaagtt cggctggctc gtgctgacga
     5881 ccgggaacaa gtcggagctc tccgtcggct acgcgacgct ctacggcgac atggccggtg
     5941 gcttcgcggt gctgaaggac gtgttcaagg ggtgggtcta ccggttggtg gagtggcgca
     6001 acgagcgcga gggccgcgag ctcgtgccga acacggtcct ggagcgcccg ccctccgccg
     6061 agctgcgcca cgagcagcgc gacgaccagt cgctgccgcc ctacgaggtg ctcgacgcga
     6121 tcctgctggg ctacgtggag ggcgacctcg acgccgacgt cctggtggcc cacggcctcc
     6181 cgcgcgagga cgtggagagg gtgatcgggc tggtggaccg cgccgagtac aagcgccgtc
     6241 aggccccgcc cggcatccgc atctccacgc gtgcgttcgg tcgcgaccgg cggctgccga
     6301 tcacgaaccg cttcggccgc taggtgtagc tcctaaccac gtttgtcatc cgggccccct
     6361 tggaggctgg gaggtgtcga agccaccagc cccaaggagg acccggatga agctccacgc
     6421 taacgcccgc acctgtccga acagccgcag gctgctcgtg gacaggatcc tgagggaaag
     6481 ttggtcggtg acggcggcgg ccgaggccgc cggcgtcagc gagcggaccg cctaccgctg
     6541 gctcgcgcgc tttcgtcagg agggggcgcc cgggctgctc gaccgctcct cgcggccgca
     6601 gcgggtccac gcgacaccgg cccgcctggt cgaggcgatc gagcggctca ggcgactgcg
     6661 gatgaccgcc gcgcagatca cggagatcct cggggtcccg ctctcgacgg tctcgctctg
     6721 gctcaagcgg ctcgggctcg gcaagcgctc gcggctcgag ccgccggagc cgccgaaccg
     6781 ctacgagcgc cgccatcccg gcgagctcgt ccacgtcgac atcaagcagc tcgggcggat
     6841 ctcgccgaag ggcgccggcc accgcgtcgt cggccaccgc gcgagccagt cgaatcgccg
     6901 cgtcggcgcc cggcagcggc gcacgacgcg cttcgaatac gtccacgtga tggtcgacga
     6961 ccacacgcgg ctcgcctacg cggaggtgct ggcaacgctc acgagccgct gcgcggtcgg
     7021 cttcctgcgc cgcggcgtcg cctggttcgc tcaccgtggc gtccgcgtcg gtgcagtcat
     7081 gaccgacaac ggctcgagct acacggctca cgctcaccgc cgcgcgcttg agacgctcgg
     7141 cctgcgccac ctgcgcaccc ggccctaccg cccgcggacc aacggcaagg ccgagcgctt
     7201 catccagacg ctcctcaacg agtgggccta cgaacgcgtc ttcggcagct cagccgagcg
     7261 cgctgccgcg ttgccgctgt tcctcgagcg ctacaactac cggcgtccac acggctccct
     7321 cggccacaag cccccggcgt cgcggctgac gaacgtcgtt gggaactaca gctaggcgat
     7381 cccgagccgg gccttcatct cgtccggcat cgggcggacg gccacggtga cgcgcgaggt
     7441 cgcgcagagc cggccccggt cgtcggtcat gtcgacgtcc cagacccacg aggtccggcc
     7501 gcggtggcgg cgggtgccca ccgcctcgac gctgccctcg aagaccggcc gcaggaagtt
     7561 caggttgtgg gacatgccct gggcgagcat cccggtcggc acgaccgcct gcgcggtggc
     7621 ctcggagacc agtccctcgg ccagcgcggc gtaggtaccg ccgtgcacga ggccgaacgg
     7681 ctgcttgtgc cgggaggcca ccgtgaagcg gcccacggcg cgctcctgac ccacctcgag
     7741 gtgctcgaac tccaggatgc cggagagcgt ttcccgcggc tcgaccggca atgacggctc
     7801 ggtgttcacg cgggcaagtt actctttcgc cgccccatgc cgactttcga tcagcttccc
     7861 gccgagcagc gcgccatcat cgagctcgtc gtccagcgcg ggcggaccta cgagcagctc
     7921 gccgacgcgc tgcagatcac gcccgagcgg gtgcgcgagc tggcgcacga cgcgctggtg
     7981 gagctctcgc cgcgcagcgc cgcacgcgtg gacgccgacc ggcgcacgca gatagccgac
     8041 tacgtgctga accagcagtc gagcgccgag gccaccgcca cgcgcacgca cctgaagcgc
     8101 tcggaggcgg ggcgcatgtg ggtctcctcg ctgctcgact cgctcgagca catgtacgag
     8161 gacggcaacg cgcccgacgt gccggatgcc gaggccgagg aggagcgtcc gcgccgccgg
     8221 gagcgggagc gcgagcgaga gcgggagcgc cccagggagc gcgagcgcac gcgtgatcgc
     8281 gagcgtgacc gtgatcgtga tcacgagcgc ggggccgccg cacgcgagcg accgcgccgc
     8341 gacgagccgc
//
LOCUS       DASVPL010000161         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_176335, whole genome shotgun sequence.
ACCESSION   DASVPL010000161 DASVPL010000000
VERSION     DASVPL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176335"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VF517_08265"
     CDS             complement(<1..298)
                     /locus_tag="VF517_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8052973.1"
                     /translation="MPRGPDHSGEAGYSMVEMMIAIFVILVGVLGTVTMIDRANKSTG
                     ETRTREAGTSLARELVETTQGLPMASVTSATINTELYSRGFADDQTGVPGWQILR"
     gene            complement(461..1231)
                     /locus_tag="VF517_08270"
     CDS             complement(461..1231)
                     /locus_tag="VF517_08270"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEX8052974.1"
                     /translation="MSNPRGNIGHILTAAGGALLALFTFMAWFDPGELSAWEIFNIVD
                     VVIFFAALGAAALAVAHLVGMAANLPPQVTSAQRWLGAVAALFAIAFIVEIVAGDGEF
                     KFGGFLAILAALVLLAGTVLLVRPDLAATVNEATANIGSGGGAGAPGAGAHQPGGMPA
                     PGGGLGGPPSPGGTPGGGLGGPPSPGGGPGGAPSPGDAPAPGPSSPAPSSPAPVSSPT
                     PASQPDPSAPAAGWYPDPQGQKRLRYWDGGRWTEQTAD"
     gene            1305..2501
                     /locus_tag="VF517_08275"
     CDS             1305..2501
                     /locus_tag="VF517_08275"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8052975.1"
                     /translation="MDPLRDIEGLVEHEGRWPGTDAERRAARHLERRLRAIGREVEVE
                     PFDVWPRWPLAHLIHALAAIVGSLVASSKPLAGAIVLLVVALLTLGDLTGRFQITRRL
                     TGRRASQNVVSREGGDLPGTLVLVAHYDAARSGAVFNDRAMRRRAWIGRVLHRPIGPF
                     EPFFWAIVASLVCALLRLAGIDGDVLSAVQFGAAVVLIVSVALLADIGLSEVVPGAND
                     NASGVATVLRLADRYGGDLEHFDVWVLLTGSEEAFAQGARAFLRRHRDELERGRTVFV
                     SVDKVGAGGVRFARREGLILARSHHPALVDLCDQIADEDADEGRYDARSIVSRSVTDA
                     VPARGAKFAVASVSCLPEAGYAPHYHQPTDLPEHIDPEALDRAFGFCSELIELIDDEI
                     GPRLRD"
     gene            2556..2864
                     /locus_tag="VF517_08280"
     CDS             2556..2864
                     /locus_tag="VF517_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052976.1"
                     /translation="MNLKALIGTTFICTALIGGAATAEAKPQEKKLKHCKQHGKKLSC
                     VTPDDDQTVEGVCPGEYEPVELVQLPPGNEVVDLNSNFILCYSDVLGVTDDKQLGKAS
                     "
     gene            3051..4433
                     /locus_tag="VF517_08285"
     CDS             3051..4433
                     /locus_tag="VF517_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052977.1"
                     /translation="MRLLALTAVVALGLAAPAQAQNACFGQTDADSVEQKPGPALRFG
                     ITPGVQTGQYVTGAVPPRTPEDPAKQVAGLAGLRPGDGPFVLRLHRFFWSGGEDEIRR
                     VLALSDLYTRAGYLVELQLRYHPNAQQEGDIGAWVEHVREVVRRFGANPRVVAIQVTN
                     EVNITFSPDSSDGSYTGARDALIEGVVAAKDEVRKRGFRQLEIGFNWAYRSEPSAENG
                     FWSYLRDHGGPRFVEALDWVGVDAYPGTVFPPAHTEGGERDGMVNAFSTFRCYAGSAG
                     IPETVPIHVEENGWPTAPPERSYEQQAKVAETLVRAVNDFRGTYNITDYRWFNLRDGD
                     TTSPNFQTQYGLMTDEYEPKPAYGVYSRLVRELTERYAKPRLTLSVRCRARRWGAAVG
                     GPDVDQVLSATFRVGDLHAPTDARAPFERSISARTFDRDRRRWNMVARVTLIDGRTAD
                     LHRRIGRCDR"
BASE COUNT          698 a         1684 c         1681 g          637 t
ORIGIN      
        1 gccgcaggat ctgccagccc ggcacgccgg tctggtcgtc cgcgaagccc cgggagtaga
       61 gctcggtgtt gatcgtcgcg gacgtgacgc tggccatcgg aaggccctgc gtcgtctcga
      121 ccagctcgcg cgccagcgac gtgccggcct cgcgcgtacg ggtctccccg gtgctcttgt
      181 tcgcccggtc gatcatcgtg accgtgccga ggacgccgac gaggatcacg aagatcgcga
      241 tcatcatctc gaccatcgaa taaccggctt cgccgctgtg atcgggcccg cgaggcatcg
      301 aggactgatc gggacgagcg accgccaact tgatagctgg cgccgggagc ccttagaaca
      361 tgcgtctagg ggcggtcaag ccccggcgca gagctgcgcg cggggcgccc cgaacccggc
      421 cgccgagcgc ggccgggcgg ggtcggacgg attgcctacg tcagtcggcc gtttgctcgg
      481 tccagcgccc gccatcccag tagcggagcc gcttctggcc ctgcgggtcc gggtaccagc
      541 cggcggccgg agccgacggg tccggctggg aggccggggt cgggctggag accggagcag
      601 gggaggacgg agccggcgag gacgggccgg gggccggtgc gtcgccggga cttggcgcgc
      661 caccggggcc gccacccggg ctcggcgggc caccgagacc gccgccgggc gtgccgcccg
      721 ggctcggcgg gccaccgaga ccgccaccgg gagcgggcat accgcccggc tggtgcgcgc
      781 cggcgccggg cgcacccgcg ccgccgccgg agccgatgtt cgccgtcgcc tcgttcaccg
      841 tcgcggcgag gtcgggacgg acgagcagca cggtccccgc caggaggacc agggccgcga
      901 ggatcgcgag gaaccctccg aacttgaact cgccgtcgcc cgcgacgatc tcgacgatga
      961 acgcgatcgc gaacagggcc gccaccgctc cgagccagcg ctgggccgag gtgacctgcg
     1021 gcggcaggtt cgccgccatc ccgacgaggt gcgcgaccgc cagcgccgcg gcgcccagcg
     1081 cggcgaagaa gatgacgacg tcgacgatgt tgaagatctc ccacgcgctg agctcgcctg
     1141 ggtcgaacca ggccatgaag gtgaacagcg cgagcagcgc gccaccggcg gcggtgagga
     1201 tgtgaccgat gttccctctg gggttactca agtccttgct ccgatctagc gttggggaag
     1261 cggcgtcact ctaaccggtt ctcgggccat actggccacc gcccatggac ccgctgcgtg
     1321 acatcgaggg actcgtcgag cacgagggcc gctggcccgg gaccgatgcc gagcggcgag
     1381 ccgcccggca tctcgagcgc aggctgcgcg cgatcgggcg cgaggtcgag gtcgagccgt
     1441 tcgacgtctg gccgcgctgg ccgctcgcgc acctgatcca cgccctcgcc gccatagtcg
     1501 gcagcctcgt cgcgtccagc aagccgctcg ccggcgcgat cgtcctgctg gtcgtcgcgc
     1561 tgctcacgct cggcgacctc accggcaggt tccagatcac ccgccgcctg accgggcggc
     1621 gcgcgtcgca gaacgtggtc tcgcgcgagg gcggcgacct gcccggcacg ctcgtgctcg
     1681 tcgcgcacta cgacgccgcg cgcagcggag ccgtcttcaa cgaccgggcg atgcgccgcc
     1741 gcgcgtggat cggccgggtg ctgcaccgcc cgatcggccc gttcgagccg ttcttctggg
     1801 cgatcgtcgc cagcctggtg tgcgcgctgc tgcgcctcgc ggggatcgac ggcgacgtgc
     1861 tctcggcggt ccagttcggc gcggccgtgg tgctgatcgt cagcgtcgcg ctgctcgcgg
     1921 acatcgggct ctcggaggtc gtcccgggcg cgaacgacaa cgcttcgggc gtcgccacgg
     1981 tcctgcggct ggccgatcgc tacggcggcg acctcgagca cttcgacgtc tgggtgctgc
     2041 tcaccggctc cgaggaggcg ttcgcgcagg gggcccgcgc tttcctgcgc cgccacaggg
     2101 acgagctcga gcgcggccgc acggtgttcg tgagcgttga caaggtgggt gccggcggcg
     2161 tccgcttcgc ccgccgcgag gggctgatcc tcgcgcgctc gcaccacccc gccctcgtgg
     2221 acctctgcga tcagatcgcg gacgaggacg ccgacgaggg gcgctacgac gcgcgctcga
     2281 tcgtctcgcg cagcgtcacg gacgcggtcc ccgcgcgcgg cgcgaagttc gcggtagcga
     2341 gcgtctcctg cctgccggag gccggctacg cgccgcacta tcaccagccg accgacctcc
     2401 ccgagcacat cgacccggag gcgctcgacc gcgcgttcgg gttctgctcc gagctgatcg
     2461 agctgatcga cgacgagatc ggcccgcggc tgcgcgacta gaacaagcgg ccaaatcagt
     2521 tcagatggcc gcgaagctga cgtatggtcg ccgccatgaa cctcaaggca ctgatcggaa
     2581 ccaccttcat ctgcaccgcc ctgatcggtg gggccgccac ggcggaagcc aagccgcagg
     2641 agaagaagct caagcactgc aagcagcacg gcaagaagct cagctgcgtg actcccgacg
     2701 acgaccagac tgtcgagggt gtctgcccag gcgagtacga gccggtcgag ctggtccagc
     2761 tcccgccggg caacgaggtc gtcgacctga acagcaactt catcctctgc tacagcgacg
     2821 tgctcggcgt taccgacgac aagcagctcg gcaaggcgag ctagccgggt gcgccgcgga
     2881 cccagtcgag cgaccgagga tgcccggcgc ccggcatgtc gccggagccc gcgcggagca
     2941 cggccgagcg cacggcgtcc cggagcaccg tcgtcgagcg cgacctcggt agcgcgccgg
     3001 cgagcacggc actagctcac ctcccccgcc ccgttcttaa gatgcggtgg gtgcgcctgc
     3061 tcgcgctcac cgcagtggtc gccctgggtc tggccgcgcc ggcgcaggcc cagaacgcgt
     3121 gcttcggcca gacggacgcc gactcggtcg agcagaagcc gggcccggcg ctgcgcttcg
     3181 ggatcacccc gggcgtccag accggccagt acgtgaccgg cgccgtgccg ccgcggacgc
     3241 ccgaggatcc ggcgaagcaa gtcgcggggc tcgcggggct gcgcccgggc gatgggccgt
     3301 tcgtgctccg gctgcaccgc ttcttctggt ccggcggtga ggacgagatc cggcgcgtgc
     3361 tcgcgctgtc cgacctctac acccgcgccg ggtacctggt cgagctccag ctccgctacc
     3421 acccgaacgc acaacaggag ggcgacatcg gcgcgtgggt cgagcacgtg cgcgaggtgg
     3481 tgcggcgctt cggcgcgaac ccgcgggtcg tggcgatcca ggtgacgaac gaggtcaaca
     3541 tcacgttctc gccggactcc tcggacggct cctacacggg ggcgcgcgac gcgctcatcg
     3601 agggcgtcgt cgccgcgaag gacgaggttc gcaagcgcgg cttccgccag ctcgagatcg
     3661 ggttcaactg ggcgtatcgc agcgagccga gtgcggagaa cggcttctgg agctacctcc
     3721 gcgatcatgg cggaccccgt ttcgtcgagg cgctcgactg ggtcggcgtg gacgcgtacc
     3781 cgggcacggt gttcccgccg gcgcacacgg agggcggcga gcgcgacggg atggtcaacg
     3841 cgttcagcac gttccgctgc tacgccggca gtgctggcat cccggagacg gtgccgatcc
     3901 acgtggagga gaacggctgg ccgaccgcgc cgcccgagcg ctcctacgag cagcaggcga
     3961 aggtggcgga gacgctcgtc cgcgccgtga acgacttccg cggcacctac aacatcaccg
     4021 actaccgctg gttcaacctg cgcgacggcg acaccacctc gccgaacttc cagacgcagt
     4081 acggcctgat gaccgacgag tacgagccga agcccgcata cggcgtgtac tcgcggctcg
     4141 tgcgcgagct gaccgagcgc tacgccaagc cgcggctcac gctctcggtg cgctgccggg
     4201 cgcggcgctg gggagcggcc gtgggcgggc ccgacgtcga tcaggtgctg agcgcgacgt
     4261 tccgggtcgg cgacctgcac gccccgacgg acgcgcgggc gccgttcgag cgctcgatct
     4321 cggcgcgcac cttcgaccgc gaccggcgcc gctggaacat ggtggctcgg gtcaccctga
     4381 tcgacgggcg gacagccgac ctgcaccgcc gcatcggccg ctgcgatcgg tgatccgtcc
     4441 gtctgagcgc tcaaggtctg cgctcagggt ccgaaaggag acttgagccc cgggggcgaa
     4501 tgccggattg ttcgccctct accccgggga accatcgacc gaagaccccc tgatgaccta
     4561 cggctacgaa ctcaccggcc gcatcgaaag cgagccgatc gacctgctcg cggagtggct
     4621 cgaggccatg atcggcctcc ggctgaccgc cttcgccacc aacgcgaagc agaacgagat
     4681 tcgccagatc gctcacggtg
//
LOCUS       DASVPL010000162        27055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_176525, whole genome shotgun sequence.
ACCESSION   DASVPL010000162 DASVPL010000000
VERSION     DASVPL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 27055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27055
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176525"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VF517_08290"
     CDS             <1..365
                     /locus_tag="VF517_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8052978.1"
                     /translation="LRALGGATVPRVTVILRQAYGGAYITMNSKELGADFSFAWPRAR
                     IGIMAATQAVGITHRAEIAAAPDPVAHRRRLAERYAAEHQSAYAAARDGFVDEIITPH
                     ETRERLCAALSTFRGKRL"
     gene            362..850
                     /locus_tag="VF517_08295"
     CDS             362..850
                     /locus_tag="VF517_08295"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8052979.1"
                     /translation="MTGVLPDTRLARALHDGVAQRLAGVAAALGSARDLSASDRARCA
                     AELSAAITELRELIEQGAGGPDAPAGGLERLISDFVVEGIRNAEKHAHPHLIAFSGYV
                     AKDRIHVEVLNDGVLPATSAEGTHVGLRLIGDEAARQGGTVAAEPLGPDGWSTRLTIP
                     RE"
     gene            complement(869..1705)
                     /gene="fabI"
                     /locus_tag="VF517_08300"
     CDS             complement(869..1705)
                     /gene="fabI"
                     /locus_tag="VF517_08300"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF005908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX8052980.1"
                     /translation="MPAQLVPRALAESRLSGARLLEGKRIVITGIATRHSLAFRVAEA
                     AQLLGAELVLTSFGRMRRMTERAARQLPEQADILELDVNDPEAFELLHGDLERRWGAV
                     DGALHAVAYAPPDAMGGNFLTTPRESAELAFRTSAYSFKALAAALAPLMSAGGSLVAL
                     DFDGSRAWPTYDWMGVCKAALESVSRYLARDLGPRGIRVNLVACGPLETLASQGVPGF
                     ADLCKTWSLRAPLGWDTGDSSVLAGPVCFLLSDLAVAISGEVLHVDGGFNAIGAVEGV
                     RH"
     gene            complement(1711..3318)
                     /locus_tag="VF517_08305"
     CDS             complement(1711..3318)
                     /locus_tag="VF517_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX8052981.1"
                     /translation="MRSFQLRIGDRDSEGIGWTYVPHAEALIRDPRRAFNAKRAMELG
                     RECDERDLALLAGRCAVGDDEDNLAALAAAAEASRELRRVSHAERKALGHALNERIRE
                     CFDDFVDVLIAEGHPRRLAEWEAEGIVQASSPEALDWAFAQISQTFHDGERRMLLTRK
                     PDGVVCINPPQNAAASNAALGLMALLAGNALVVKAPRTAPLGVMYLFREVVLPVLDDR
                     GMPPGTLNLVSGYSKRIVKTWVESPHVDDIMWFGESAAGLRFGQECFASGKKPILELS
                     GNDAFLVWSDADLDRAAKALTESFYGSSQICMVPKQAILHPAIASEFTELFLARVAEI
                     RPAYPSEPGAVLSPVLKMDTFFDFLGEAEQDGCELLCGGERVGVDGNPAVDGAFIEPT
                     VVLVRGLAEARRLRCVREETFFPLLPLVVADDCDGDGELLDTVLDWMNDNPYGLRNSV
                     WATDPRVTDSFATGLVNGGLLKVNESHVAFSAYLGTHGGTGLTGGPFGELHYPLFRAS
                     HMQGIAFGDGTQSLEAPEPSLAAAIEP"
     gene            complement(3315..4667)
                     /locus_tag="VF517_08310"
     CDS             complement(3315..4667)
                     /locus_tag="VF517_08310"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX8052982.1"
                     /translation="MKFVERVDLPAADAPTRWYNVLPDLPRLPEPYLDGRHGEPVTPE
                     YMARMSPRSIAAQEFSLERWIPIPQEVIDAYRLWRPTPLYRALALERSLDTPAEIWFK
                     YEGSSPSGSHKLNSALAQAALARNDGVKRLVTDTGAGQWGTALALAGSLMGIEVEVFM
                     VRASYHGKPYRRYLMETYGATVHPSPGDETEYGRSVLAETPDHPGSEGCAVTEALEAV
                     HRDPDARLSMGAFGNHVLLHQTVIGLELREQLEQIGRTPDYLVASVGCGSNMGGFCLP
                     FVPDKLAGADIRMVAAEPSACATLTKGEYRYDFADATGAGIAAKTYTLGHDFVPPPIH
                     AGGLRYHGAAPLMGLLRDEGLVDAVAFDQLEVFEAGSEFARLHGLLPAPETAHAIRSV
                     IDIATDCRRTGRKATIVFCYSGHGLLDLEAYGRYQAGQLQSSADEVFDDRPLEPVPVD
                     "
     gene            complement(4678..6333)
                     /locus_tag="VF517_08315"
     CDS             complement(4678..6333)
                     /locus_tag="VF517_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8052983.1"
                     /translation="MSLLKGQLAPRISLRPPLSIGILPERAAETFGRVPIHLDAPFEF
                     DPEQRVDLDYVEFAQLVEQLSGALHEAGVRPWDRVAVAKSSNFDIMALAAAAARIGAI
                     PALLSPRLDGEIMGVLLDRLDSPYLYTDRATVERAGLSGKRLAGMAARVIGDVDGAVP
                     IADLWGAPAPAPAPRSGGEPMLVTHTSSTTGISKLVENSVEGVCFSARVEAMVPFGHS
                     PRELAASCISFVHVRAAITAMASLTRGTALLEVGNVTDENVIRLFHRHRPTAVEAHPN
                     TFLAWERLCDHPTEPFSNLRIYFSTFDAAHPRTISRLLAASRRTLPLWCQAYGQTEVQ
                     IVSFRFYTRRTARRLMRSGTKSRSLGWPAPGVRVRIADPATQRRMRRGQPGMIQVRTP
                     AQALGFVGTPAKYWQRRHGSWFDTGDWGRRTRWGDVEIFDRMADRIEGVESCLWLEDM
                     LLERIPDAEEIVIVPDKQGRPVPVVCMREGEPLDDARWRAASAGIPNLASPFQVSEGD
                     LKRTATAKARRYLLTELIAARDAVSENGDHPVMAPEILLREGS"
     gene            complement(6478..7875)
                     /locus_tag="VF517_08320"
     CDS             complement(6478..7875)
                     /locus_tag="VF517_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8052984.1"
                     /translation="MRNGGQTLEADVVVVGAGLAGLTAALELKRAGRTVVVLEARTRV
                     GGRLLGHPVGDGKFIDLGGEYFGDKSVAIADTARSVGVRSFESHDEGERITYYLGRRV
                     TYRGIVPWVNPAVLADFGQAVVRIERLVRGVPAGRPWEAPRARELDGQTFWAWLRRNT
                     ATSGARALMTLGTECAFCATPSDLSFLHVLNYAKASGGFLYLMDVSGGIQKYRFETGT
                     QSVAQRMAAELGDDVHLGAVVRHVRHDAESVVVGGPGFQVEARRAVVAIPVTLAGRIA
                     YEPALPGHLDQLMQRMPAGAAIKCIAIYDEPFWRAEGLTGQATSPDGPVRVTFDASPA
                     DGRPGALSAFVVGSAARAMTRLSPPERREAVIGALVRFFGPRARTPVEFVEQNWMDEE
                     FTRGCYHGFAPPGLYTAYGPALRAPVGRIHWAGTERGVHQLGSMGGAVDSGRRVAREI
                     LASADRAREAVAAGA"
     gene            complement(7898..9310)
                     /locus_tag="VF517_08325"
     CDS             complement(7898..9310)
                     /locus_tag="VF517_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8052985.1"
                     /translation="MPGERFDVVIVGARCAGSPLATLLARSGLRVAVVEQVTFPRTVL
                     SSHLIEADSLNFFKRLGILDPVRKTGVRFMHGVDTRLNELRLVTRFPLRPGDLGGAAF
                     LRRHLLDSILADAAAEAGAEMRMDTKVVGLLEEAGRVTGVRVVHDGRESELHAPLVVG
                     ADGRKSTVARLCGARRYNVTRNERSYYFTFFEGASPDSDDLFVFHRWDDRMVWAGAAD
                     SGLYLVGVSPEDHQREDFRRDPETNLMAHMRACEPTAAALADARRATKIFGIVRFEGY
                     FREPTGPGWVLVGDAGHFKDPAAGRGIGDAFIQAERLAPTIVAGLDGTGPPLDLALAE
                     WAAWRDRKFAGHYWMTNDLGCAGAIPTMAPQLVQALEGRGEVDRFFDLFSHRSCYYDI
                     LTIPRLAEATGRVLVKGRFDRAAVVREAGRLLVREGRRRWINTHPDFEPPEPPDARPR
                     SQGTGDRVAAAARPFAAAVD"
     gene            9453..10763
                     /locus_tag="VF517_08330"
     CDS             9453..10763
                     /locus_tag="VF517_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8052986.1"
                     /translation="MARAAVPPGPHVALVQVARFSRNPLRFLESMRRRYGDVFSVPYP
                     MFGRPVYMADPAGVQTMLAGDPAVFHAGEANAGPLGPVMGARSVFLVDGEDHMRKRKL
                     LLPPFHGRRLRQYGQTIAELAAREVRGWPRGERFALRPRMQALTLDVIVRIVFGVTDP
                     ERLALYRQRVPRLGEVADPVMWIPVARRDLGRFSPWARFVRARDAVDELIYDEIERTR
                     RDPRLEERDDILAVLLQARHDDGSAMSREELRDQLMTVIAAGHETTATGLCWLFERVL
                     RHAEVEERLRAELAAGDDAYLEATATETLRVRPVVTDIARRVKRDVEVAGWTLPAGTF
                     AIPSIALIHTREDLYPEPHAFRPERFLDTKPGTYTWIPFGGGARRCIGAAFAMLELKT
                     IARTILDRTRLRAPDPRPESPRARNVTIVPSRGAEVVMEASSPG"
     gene            10840..11802
                     /locus_tag="VF517_08335"
     CDS             10840..11802
                     /locus_tag="VF517_08335"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX8052987.1"
                     /translation="MLESLTPTSWPAEVDDVVAGDLSVMFATVTPARGVVLTPFANIA
                     LVDRGAGTVTITTSLGLWGKLERIQRNPRVALAFHTRANGASERPEYVLLQGEASFSW
                     VPDRSWLESIGDNWESHLDTRRQGRAWDWWLSVYHWERVGIEIKVARTVVWPDLDCAG
                     PPAVHGPELPGPAPAQRPPARGTGSRINHARAAARIRRLPHTLLGWVGADGLPMVVPV
                     GLRGANPLGILLEAPPDVVPPGGRRAGLTAHWCGARVIGHEQRAHTGWLEPETERLLL
                     YAPHTKNGYRLPPSTPLVRAATGFATRRGFRAGRRAGVLTAQPR"
     gene            complement(11822..13813)
                     /locus_tag="VF517_08340"
     CDS             complement(11822..13813)
                     /locus_tag="VF517_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8052988.1"
                     /translation="MFAWVALAVVGGVVAAAGGRLANGGTDVPGSQSARTDALEADRI
                     FGEDRTHVFVVVSVPEGRASSLAPLLPRVRKAVAAAPHVAEVGQGAVVSRANFAIFAF
                     DLGSTLVDAQDQLADVRTAAKRAAGQADVTIVGEAADYDRYGTIARDDLDRVEKISFP
                     VTSAIILVAFLSIVATGVPTVLAGLVLAVTFGLLFAISQLTTLSVFASNTALGLGLGL
                     SIDYSLFNVTRYREALRENGGSIPDAVRETVSTTGRAVVFSGLTIITATASLFLLQVG
                     VFSGMAIGVIAAALVAALGGVTLVPAVLVLAGRHLDRLALRRVATAAESGRAWKRLAA
                     FVLRRRLPVAVGAATALALLTIPLLHAPLASPTASALPKNDRVRVVSERVGRVFGGGA
                     VTPVQVVARGNRKRVAQALERDPGVGRPIVIRPGKDGWLLLLAPLLDNGDSPAAQETV
                     RRLRRKLTEGDAPAAAAVGGTTAKAVDLLDRLHDRTPAVVATALLLSATLLLFAFRSI
                     VVPIKAALTTLLSVGAALGILTLLYHDLGIGESDRLSFFVPLFLFAIIFGLSADYEVF
                     LLSRIREEYLSGASSDRSIERGLVASARAITLAGLVMVAVFLAQASSRMEPLQQLGLG
                     MAIAVLIDITIVRTFLVPATMALLGRANWWLPGRRNRGQ"
     gene            complement(13973..14479)
                     /locus_tag="VF517_08345"
     CDS             complement(13973..14479)
                     /locus_tag="VF517_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8052989.1"
                     /translation="MPTREASPATSTNADLVRRAFEAVNRHDARAASELWSEDALWRV
                     PEQTIRGPRQVAAWFEELFAAMPDVRFDVIAIAAEGPDVFVHWHMAGRHTGRLTGLDG
                     TGRSIALDGMDHLVVRDGKIAANTIVFDQLESARQVGALPPLGSRADRLMTAMLNVRT
                     KLVSSLRR"
     gene            14630..14797
                     /locus_tag="VF517_08350"
     CDS             14630..14797
                     /locus_tag="VF517_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052990.1"
                     /translation="MTDDPQEKQPQPVEDGTPEDAPEYAPAEKDKSIADGSHSAAAIG
                     EAGPAGERVDG"
     gene            14935..15441
                     /locus_tag="VF517_08355"
     CDS             14935..15441
                     /locus_tag="VF517_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8052991.1"
                     /translation="MEAPAIRELADELLARWNAHDVDGVAALCSEDVVFVDPALPQPA
                     IGRRAMRDTVASALVAFPDFRLERIGDPLIAADEGLVLVRFRMIGTMKGHWGAANVAA
                     TGRSLDIRGIDEWLVSDGLLRSCVAHYDSTEAARQLGMLPPETGLMNRAMLRMQHVQA
                     WFQRRSSR"
     gene            complement(15448..17265)
                     /locus_tag="VF517_08360"
     CDS             complement(15448..17265)
                     /locus_tag="VF517_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8052992.1"
                     /translation="MSAERTEVVVVGAGLAGLTAALDLRAGGHEVVVLERGDRAGGRV
                     RTAELDGLAVDLGAHFVSPRYRTVLRLIERAGLESALRPIGPSLCTAVFVGGRFHYLD
                     FGRPLEVARYSALSPAQRARLGLMALPLLRSAPSARFFDMASVAAVDRVAPRRLLGTD
                     AVERVFAPLSQALCGYSIDQVSLPLLTLGARFPLGRPLSLDGGLGTLGPALARDLPVR
                     LGCEVTRVEREGERVLVHTGGDGFEADAVVLATTAPAALRAWPGAEGPARELLAATRY
                     SRHFHAYFKTTGHHTRAAPDGRAVYMEVFPGGSARGPISHISFAATDPAAHAGLVFVA
                     AHPDAVANIPDDEELAGALQEQLERLHPELAADVTGRATVRWEEKVPLFPRGRARELA
                     RMRDGIDGPVQLAGDYLYGPLIEAAALSGAAAARRVGARLAARRASAQLATGRAGAQP
                     ATGGARVAGGARDDHAALAETLRWAWSRPTLDEALAATRPLLHPDAVLSQPLVRTGRG
                     FRSFERNMHGLARIFGDLRLRVVGWESAGDAVWVSFEITGTVGRRRLRVKARDRLVLS
                     GGLVSERHVTMSRWGLVRPILATPSAWPRAVAVFLRSWV"
     gene            complement(17255..19792)
                     /locus_tag="VF517_08365"
     CDS             complement(17255..19792)
                     /locus_tag="VF517_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8052993.1"
                     /translation="MPGARGATQAWLARPLRRAVRLLLAVLGVAVGVGLLSAVLSQNG
                     STTRGAEDAYKRLAGRARVEVVARSPLGMPVSVERRVLRSRSVALAVPTVHDLVGVET
                     GRGRFDLWLIGATSDAEQLGGAPATFARAGAERRGGGSAGGGLVVSRAIATRLGVGAG
                     DRVTVRSRVGIRRARVGAVLPDERLGDVADAPIVAAPLALAQRLDGNPGRIQRIAVVP
                     RGSADDTRAELRRLIGDRVADVRGADFDASAQAQASSLIRNSSSLFAALSLVVGALLA
                     YASMSLIAAERRREVATLNALGCSSRALLVAVLAEALLVGLAGSVLGVALGRAALGGL
                     LPAPDPLLASVFIVERTARMTPGVVALSVAAGVAATLAAAAVPARELVRVPPAEALVG
                     EGTAAASPGRLTGSVWLVAAPIVLALAGAALSLAGQAIVGVGLLVFAGLLAIPAAVPV
                     VARAIRARLRSPGGAPLVGITEIVAFPSRASALVAVVTLAVCGTTIIAGTASDLSTGV
                     GRLGGSTYKGAAVFVRPDSEGNVFLTQPIDPRLRSTLERRPKVGSISEWRASFISWNG
                     RRILAFGFDPDQRDSPSGFTRGDGRRAAKALTTDPAAVAVSSDLGGGASPRPGSRLTV
                     PTPVGPRHVRVVANISNYGWAAGAIVFNPASLARWWGRDFVTALKLQPEPGVGPGELA
                     RGLEPVLRRRGLTASTSPAMRAQAKSNAEEQLATVSRIGSLIALAGVLAVAAAAMLGV
                     LGRARRITSLRTIGMSSRQVASATFAELACIVLIGALVGLVLGIVGRVLAVHHLATSV
                     SLPVDLYISWPQFVRPVVLVAVIAGVATLLAVRASLRLPLRRALDER"
     gene            complement(19798..20535)
                     /locus_tag="VF517_08370"
     CDS             complement(19798..20535)
                     /locus_tag="VF517_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8052994.1"
                     /translation="MSVVSAERPAGEAPALRLDHVTRVFRQGANEVHALADVSMSIYP
                     GEVTAVMGPSGSGKTTLLQIAGAMDLPSSGRVFHGETDLAGLDAAGLSRLRRQRIGFV
                     FQFFNLMPGLTALENVALVARLDNVSRAKSAERARALLARVGLEGRADHLPSALSGGE
                     QQRVAVARALVNRPRLVLADEPTGNLDRRAGASVVEVLVEAAREQRAAVLIVTHDAVV
                     HDYVERTMTMADGRVSDSGEVARTAQA"
     gene            complement(20532..21827)
                     /locus_tag="VF517_08375"
     CDS             complement(20532..21827)
                     /locus_tag="VF517_08375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8052995.1"
                     /translation="MKDRQAATSRTRSAPTFDAVVVGASLAGSTATKLLAEQGLRVAL
                     VERQPDASAYKRVCGHFVQSSAMPALERLGVVDAMLEAGAVRSRFRVRTPWGWIEPPP
                     PTSAPAGINLRREHLDPMLRERAAAAPGVELLLGQRVTGLVHQSGTVAGVRITARDGS
                     ERTLRSRLVVGADGRDSAVARLAGLRTHRRRHGRFAFSAYYAGPKPAGSPDASVWCGG
                     VPWGMAFPTDSDLTLYVAMDSLERRAPFRADPAGALEDFIASRADPPPIRDSERVSDV
                     VGRIDMTSIRRAPVGSGVALAGDAALAADPLWGVGCGWAVQSGDWLAGCVGPALNGAE
                     PLRAGLARYRARRRRALLPHALLIESYATGRELYPIERLMFAGGVHDRSVAERLNALV
                     TRNAGPLHVGGQLAVPRAILAVTRIRNAGAARELPQPVA"
     gene            21936..22820
                     /locus_tag="VF517_08380"
     CDS             21936..22820
                     /locus_tag="VF517_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX8052996.1"
                     /translation="MKDRISGLVRLLRPYSITWTASSVVVMVILLTGGRFSTMDLVGC
                     LFAMSAIGGAARTLNDVADRATDELSTEADRRGRPLVTGVVSARMALAQVVLLGGAGI
                     AVAFAVDLGFGIFMGLGAMGLVLYSAGPAPVAGLPLSQGFWIAFWTTVYLSLYLALDG
                     DVGRGVPYLISTCLFMGIGETIAKDLRDLENDAQGGRRTTPLLLGPRRSVYACFLAYA
                     AGGAGFVVTAALRSPDVRLPIAVGCVALLWVARCVQSLNATRDGYVKEEARVLHVGSV
                     RVFLVVNLLLLAGIPLAD"
     gene            22810..23832
                     /locus_tag="VF517_08385"
     CDS             22810..23832
                     /locus_tag="VF517_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8052997.1"
                     /translation="MPTEPASVAAPERLWPVAELEPDRVGDALRRLLNEAGPGALPRT
                     ADDVLASHRCADRLAAAGLTEADGTPRFRVNALAGRIFVTDLPEATRAGGAFPYADEA
                     ERLLAYLERSGVSTAGAHLVDVGAGCGHVTLSVAAARRTAVEVNPRARELLALNARLN
                     ALEAEVVSGPAQSQLAGLDPIVSPAGETVVVAILPHAPAPDSSMLAGFANGGRTGAAV
                     IEAVLEELAAVAARAVRVVMLCYSLRSSETEATLVPRAARRLMPDRNVEWTLLAGAPM
                     WRIDGVMSERSPMALVPALARKAQCRFYCDADQRESTAAAYSELATELAADGWDQLCY
                     GALDVR"
     gene            complement(23829..25454)
                     /locus_tag="VF517_08390"
     CDS             complement(23829..25454)
                     /locus_tag="VF517_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX8052998.1"
                     /translation="MATSERTVDVLVIGAGAAGLAAALAATRAGAEVMVAAAGTPGES
                     HSVKAVGGMNGVFDREADWREHVHDTLRVGDGLADRAAVERMCASASEVVAGLLSDGV
                     EMRRELLGRTAHWQYTLPDITGLTIVEALAGALERSGAQLRWGVRARALAVSGGGCRG
                     AWLASGRDELHVRASAVVLATGGYGALLSPSIAAAEADGSGLGLAYAAGAELMDMEFV
                     QFHPTVAAGSPVLVSERARGAGARLLNGLGERFMERYDPERCELAPRALVSQAIDEEL
                     RAGRGCPEGGVLLDFAAVPAENWQHIEFVRRELARAVGVDVRERPVRVEPAVHYTMGG
                     VAVGLDGRTNVPGLWAAGEAACVSVHGANRLGGNSLLDATVFGTRAGVAAAATAGDTA
                     AAAPAGSGATAAAAPPGNPATEGAPAASGAAPPGDTPRPERAPDYRVGPELRALLGRC
                     AGIRRDGDALAEGLATLRASWERDRQPAAAIAAAIVASALAREESRGAHQRTDHPMRD
                     DERWLAHSVVRRGADGFEPELSLRAVDAAVPAG"
     gene            complement(25461..26153)
                     /locus_tag="VF517_08395"
     CDS             complement(25461..26153)
                     /locus_tag="VF517_08395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8052999.1"
                     /translation="MQTIAEPTIVSRTKAAYDGVGGALWTLNHRIAPVWVLYGAIPWG
                     SDLRKGEREVERFTQSWESGRVILDLPVGMGRMFPHYADRLKPSRLIAVDLSDGMVRR
                     ARRSAERAGFSGNGDFVAADVASIPLPDASVDYVLTEGGFHHFPDREAAVSEFMRVLR
                     PGGSIAGYALVDRENLRGSLSLRICHRFSLMAEPISAARLRSVFHAHAAADWQESKTG
                     SLLCFAARKPEQ"
     gene            26297..>27055
                     /locus_tag="VF517_08400"
     CDS             26297..>27055
                     /locus_tag="VF517_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX8053000.1"
                     /translation="MALIGDRPEGEAEWPLADACGHAVTHTGKQLRPRLVAEASRLGS
                     PPDEEVVKAMCAVELLHVATLSHDDVVDATPLRRGAPSVEGRFGAFAAEYAGGWMLGR
                     ALELAAELGEPALAQFSDAVCELCEGEMLETQELRNPGRSEESYLAAIEGKTASLFRL
                     SALLGGTLAGVDARALGALREYGHWLGMAFQLADDLGDLLADAAATGKERGTDLRQGV
                     FTLPVVYALGSDPELAALLCDEIDAAAVDAIVDRI"
BASE COUNT         3620 a        10079 c         9972 g         3384 t
ORIGIN      
        1 tgctgcgcgc gctcgggggc gcgaccgtgc cgcgggtcac ggtgatcctg aggcaggcgt
       61 acggcggcgc gtacatcacg atgaactcga aggagctggg cgccgacttc tccttcgcgt
      121 ggccgcgcgc gcggatcggg atcatggccg ccacccaggc ggtcgggatc acgcatcggg
      181 ccgagatagc cgccgcgccg gatcccgtcg cgcaccggcg ccggctggcg gagcgctacg
      241 cggcggagca ccagagcgcc tacgcggccg cgcgcgacgg gttcgtcgac gagatcatca
      301 cgcctcacga gacgcgcgag cggctgtgcg ccgccctctc gaccttcagg ggaaagcgcc
      361 tgtgaccggc gtgctgcccg acacgcgact cgcgcgtgcc ctccacgacg gggtggcgca
      421 gcggctcgcc ggggtggcgg ccgcgctcgg ctccgcccgt gatctgagcg cgagcgaccg
      481 cgcgcgctgc gccgctgagc tgagtgctgc gatcaccgag ctgcgtgagc tgatcgagca
      541 gggcgccggg gggccggacg ccccggccgg cggcctggag cggctgataa gcgacttcgt
      601 ggtcgagggc attcgcaacg cggagaagca cgcgcacccc cacctgatcg ccttctccgg
      661 ctacgtggcg aaggaccgga tccacgtcga ggtgctgaac gacggcgtcc tgccggccac
      721 gagcgccgag ggcacccacg tcgggctgcg gttgatcggc gacgaggccg cgcgtcaggg
      781 cggcacggtc gcggccgagc cgctggggcc ggacggctgg agcacgaggc tcacaattcc
      841 gcgggagtag gcccgtgagg cggggccttc agtggcgtac gccctcgacg gcgccgatgg
      901 cgttgaagcc gccgtccacg tgaaggacct cgccgctgat cgcgacggcc aggtccgaca
      961 gcaggaagca gacggggccc gcgagcacgg acgaatcgcc cgtgtcccac ccgagcggag
     1021 cacgcaggga ccacgtcttg cagaggtccg cgaagcccgg cacgccctgc gaggccagcg
     1081 tctcgagcgg gccgcacgcc accaggttca cgcggatgcc gcgcggtccg aggtcgcgcg
     1141 cgaggtagcg gctgaccgac tcgagcgccg ccttgcagac gcccatccag tcgtaggtcg
     1201 gccacgctcg cgacccgtcg aagtcgaggg ccacgaggct cccgcccgcg gacatcagcg
     1261 gggcgagcgc cgcggccagc gccttgaacg agtaggcgct cgtccggaac gccagctcgg
     1321 cgctctcccg cggcgtggtg aggaagttgc cgcccatggc gtccggcggc gcgtacgcga
     1381 ccgcgtggag cgcgccgtcc accgcccccc agcggcgttc gaggtcgccg tgcagcagct
     1441 cgaacgcctc cgggtcgttc acgtcgagct cgaggatgtc cgcctgctct gggagctgcc
     1501 gcgccgcccg ctcggtcatg cgccgcatcc ggccgaagct cgtcagcacc agctcggcgc
     1561 cgaggagctg cgcggcctcc gccacccgga acgcgagcga atgacgcgtg gcgatgccgg
     1621 tgatcacgat gcgcttgccc tccagaaggc gcgcgccgga gagtcgcgac tcggccaggg
     1681 cgcgtggaac gagttgggcc ggcatgctcg tcatggctcg atcgccgcgg cgagcgaggg
     1741 ctccggcgcc tcgaggctct gggtgccgtc accgaaggcg atgccctgca tgtgcgacgc
     1801 gcggaagagc gggtagtgga gctcgccgaa cggccccccg gtcaaccccg tgccgccgtg
     1861 ggtgccgagg tacgcggaga acgcgacgtg ggactcgttc accttcagga gcccgccgtt
     1921 gacgaggccg gtcgcgaacg agtcggtcac gcgggggtcc gtcgcccaga ccgagttgcg
     1981 cagcccgtac gggttgtcgt tcatccagtc gagcacggtg tcgagcagct cgccgtcgcc
     2041 gtcgcagtcg tccgcgacga ccaggggcag cagggggaag aaggtctcct cccgcacgca
     2101 gcgcagccgg cgcgcctcgg ccagcccgcg gaccagcacc accgtgggct cgatgaacgc
     2161 gccgtccacc gcggggttgc cgtccacgcc cacgcgctcg ccgccgcaca gcagctcgca
     2221 cccgtcctgc tccgcctcgc ccaggaagtc gaagaacgtg tccatcttca ggacggggct
     2281 cagcacggcg ccgggctcgc tcggataggc cggccggatc tccgcgaccc gggccaggaa
     2341 gagctccgtg aactcggacg cgatggcggg gtggaggatc gcctgcttcg gcaccatgca
     2401 gatctggctc gagccgtaga acgactcggt cagcgccttc gcggccctgt ccaggtcggc
     2461 gtcgctccag acgaggaacg cgtcgttgcc ggacagctcg aggatcggct tcttgccgct
     2521 cgcgaagcac tcctggccga accgcagtcc cgccgcgctc tcgccgaacc acatgatgtc
     2581 gtcgacgtgc gggctctcga cccatgtctt gacgatccgc ttcgagtatc cgctcacgag
     2641 gttcagcgtg cccggcggca tgccgcggtc gtcgagcacc gggaggacca cctcgcggaa
     2701 gaggtacatc acgccgagtg gagccgtcct cggggccttc acgaccaggg cgttgcccgc
     2761 gagcagcgcc atcaggccca gcgcggcgtt cgacgcggcc gcgttctgcg gcgggttgat
     2821 gcacaccacg ccgtccggct tgcgggtgag cagcatccgg cgctcgccgt cgtggaacgt
     2881 ctgggagatc tgcgcgaagg cccagtcgag cgcctcgggg ctgctcgcct gcacgatccc
     2941 ctcggcctcc cactcggcga gccgccgcgg gtgcccctcg gcgatcagca cgtcgacgaa
     3001 atcgtcgaag cactcgcgga tgcgctcgtt cagcgcgtgc ccgagcgcct tgcgctccgc
     3061 gtgcgagacg cgccgcagct cgcggctcgc ctcggcggcg gccgccagcg cggcgaggtt
     3121 gtcctcgtcg tcgccgacgg cgcaccgtcc tgcgagcagg gcgaggtcgc gctcgtcgca
     3181 ctcgcgtccg agctccatcg cccgcttggc gttgaaggcg cgccgcgggt cgcggatcag
     3241 cgcctccgcg tggggcacgt acgtccagcc gatgccctcg ctgtcgcggt ccccgatgcg
     3301 gagctggaac gacctcaatc gaccggcacc ggctcgaggg ggcggtcgtc gaacacctcg
     3361 tcggcgctcg actggagctg cccggcctgg tagcgcccgt acgcctcgag gtcgagcagg
     3421 ccgtggccgc tgtaacagaa gacgatcgtc gccttgcgcc cggtccgccg gcagtccgtt
     3481 gcgatgtcga tcacggagcg gatcgcgtgg gccgtctccg gcgccggaag caggccgtgc
     3541 aggcgcgcga actccgagcc ggcctcgaac acctcgagct ggtcgaacgc caccgcgtcg
     3601 acgagcccct cgtcgcgcag cagacccatc agcggagccg cgccgtggta gcgcagcccg
     3661 ccggcgtgga tcggcggcgg cacgaagtcg tgaccgagcg tgtacgtctt ggcggcgatg
     3721 cccgccccgg tcgcgtcggc gaagtcgtag cgatactcgc ccttggtgag ggtcgcgcag
     3781 gcgctcggct cggcggccac catcctgatg tcggcgccgg ccagcttgtc cgggacgaac
     3841 ggcaggcaga agccgcccat gttgctgccg cagccgaccg aggcgaccag atagtccggg
     3901 gtgcgtccga tctgctcgag ctgctcgcgc aactcgagcc cgatcaccgt ctggtggagg
     3961 agcacgtggt tgccgaaggc gcccatcgag aggcgcgcgt ccgggtcgcg gtggaccgcc
     4021 tcgagcgcct cggtcacggc gcagccctcg ctgccggggt ggtccggcgt ctcggcgagc
     4081 acgctgcggc cgtactcggt ctcgtcgccc gggctggggt gcaccgtggc gccgtacgtc
     4141 tccatcaggt agcggcggta cggcttgccg tggtagctgg cccggaccat gaagacctcg
     4201 acctcgatcc ccatcagcga gcccgcgagg gcgagcgccg tgccccactg gcccgcgccg
     4261 gtgtcggtca ccaggcgctt cacgccgtcg ttgcgggcga gggcggcctg cgccagcgcc
     4321 gagttgagct tgtggctgcc cgacgggctc gacccctcgt acttgaacca gatctcggcc
     4381 ggggtgtcga ggctgcgctc gagcgcgagc gcgcggtaga gcggcgtcgg gcgccacagc
     4441 ctgtaggcgt cgatgacctc ctgcgggatc gggatccagc gctcgaggga gaactcctgc
     4501 gccgcgatcg aacgcggcga catgcgcgcc atgtactcgg gggtgaccgg ctcgccgtgc
     4561 ctgccgtcga ggtacggctc cggcagccgc ggcaggtccg gcaggacgtt gtaccaacgg
     4621 gtcggcgcgt cggcggcggg caggtcgact ctctccacga acttcatcgc ttctccctca
     4681 tgagccttct cggagcagga tctcgggtgc catcacgggg tggtcgccgt tctcggacac
     4741 cgcgtcgcgc gcggcgatca gctcggtgag caggtagcgc cgggccttcg cggtggccgt
     4801 ccgcttcagg tcgccctccg agacctggaa cggactggcg aggttgggga tgccggccga
     4861 cgcggcgcgc cagcgcgcgt cgtcgagggg ctcgccctcg cgcatgcaca cgaccgggac
     4921 cgggcggccc tgcttgtcgg ggacgatcac gatctcctcg gcgtccggga tgcgctcgag
     4981 cagcatgtcc tccagccaca ggcagctctc gacgccctcg atgcggtccg ccatccggtc
     5041 gaagatctcg acgtcgcccc agcgcgtgcg ccggccccag tcgcccgtgt cgaaccacga
     5101 gccgtgcctg cgctgccagt acttcgccgg cgtgcccacg aacccgagcg cctgcgccgg
     5161 cgtgcggacc tggatcatcc cgggctgccc gcgccgcatc ctgcgctgcg tcgccgggtc
     5221 cgcgatccgc acccgcacgc ccggcgccgg ccagcccagg ctccgagact tggtcccgct
     5281 gcgcatgagg cgccgcgcgg tccggcgcgt gtagaagcgg aacgacacga tctggacctc
     5341 ggtctggccg taggcctggc accagagcgg cagcgtccgc cgcgaggcgg cgagcagccg
     5401 cgagatcgtc cgcgggtgcg cggcgtcgaa ggtgctgaag tagatgcgga ggttcgagaa
     5461 cggctcggtc gggtggtcgc agagccgctc ccacgcgagg aacgtgttcg ggtgggcctc
     5521 gaccgcggtg ggccggtgcc ggtggaacag gcggatgacg ttctcgtcgg tcacgttgcc
     5581 gacctcgagc agcgcggtgc cgcgggtcag cgacgccatc gccgtgatcg cggcccgcac
     5641 gtgtacgaac gagatgcagc tcgccgccag ctcgcgcggg gagtgaccga acggcaccat
     5701 cgcctcgacc cgcgccgaga agcagacccc ctcgaccgag ttctcgacga gcttcgagat
     5761 gcccgtggtg ctggaggtgt gcgtgacgag catcggctca ccgccgctgc gcggagcggg
     5821 cgcgggggcg ggcgcgcccc agaggtccgc gatcggcacg gcgccgtcca cgtcgccgat
     5881 cacccgggcc gccatcccgg ctaggcgctt tcccgacagc ccagcgcgtt cgaccgtcgc
     5941 cctgtcggtg tacaggtacg ggctgtcgag gcggtcgagc agcacgccca tgatctcccc
     6001 gtcgagcctc ggcgagagga gcgccggaat cgcgccgatc cgagccgcgg cggcggcgag
     6061 cgccatgatg tcgaagttcg agctcttcgc gaccgcgacc cggtcccacg gcctcacgcc
     6121 ggcctcgtgg agggcgccgg atagctgctc gacgagctgc gcgaactcga cgtagtcgag
     6181 gtcgacacgc tgctcggggt cgaactcgaa cggcgcgtcg aggtggatcg ggacgcggcc
     6241 gaacgtctcg gcggcacgct ccgggaggat gccgatgctc agcggagggc ggagtgagat
     6301 ccgcggggcg agctgaccct tcagcaggct caccggcccg tcgcggcggt ctgatttcgt
     6361 cgtcgcccgc aagggcggcg aagttatgtc cggcccccgc cgccgtgggt gggcggcgac
     6421 gcgatttcac ccagggggtg gcgatatgcc acctggcggc acggagccca gatcgcgtca
     6481 ggcaccggcc gccaccgcct cgcgcgcacg gtccgcgctc gcgaggatct cgcgagcgac
     6541 ccgccggccc gagtccaccg ctccgcccat cgagcccagc tggtgcacgc cgcgctcggt
     6601 gcccgcccag tggatccggc cgacgggtgc gcgcagcgcc ggtccgtacg cggtgtacag
     6661 gccgggcggc gcgaagccgt ggtagcagcc gcgggtgaac tcctcgtcca tccagttctg
     6721 ctcgacgaac tcgaccggcg tgcgcgcccg cggcccgaag aaccggacga gcgcaccgat
     6781 gaccgcctcc cgccgctcgg gtggggacag acgtgtcatc gcgcgcgcgg ccgacccgac
     6841 cacgaaggcg gacagggcgc cgggccggcc gtccgccggg gacgcgtcga acgtcacgcg
     6901 caccggtccg tcggggctcg tggcctgtcc ggttagtccc tccgcccgcc agaacggctc
     6961 gtcgtagatc gcgatgcact tgatggccgc gccggccggc atccgctgca tgagctggtc
     7021 gaggtggccc ggcagcgccg gctcgtacgc gatccgtccc gcgagcgtga ccgggatcgc
     7081 cacgaccgcc cgcctcgcct cgacctggaa gccggggccg ccgaccacca ccgactcggc
     7141 gtcgtggcgc acgtgccgga cgaccgcgcc caggtggacg tcgtcgccga gctcggcggc
     7201 catccgctgg gccacgctct gcgtccccgt ctcgaagcgg tacttctgga tcccgccgct
     7261 gacgtccatc aggtagagga agccgcccga ggccttggcg tagttgagga cgtgcaggaa
     7321 cgagaggtcg ctcggcgtcg cgcagaaggc gcactcggtt cccagcgtca tgagcgcgcg
     7381 cgccccggat gtcgcggtgt tccggcgcaa ccaggcccag aacgtctgac cgtccagctc
     7441 gcgcgcccgg ggggcttccc acggcctgcc ggcggggacg ccgcggacca gccgctcgat
     7501 ccgcacgacc gcctggccga agtcggcgag cacggccggg ttgacccacg ggacgatccc
     7561 ccggtaggtc acgcgccggc cgaggtagta ggtgatgcgc tcgccctcgt cgtggctctc
     7621 gaacgaccgc accccgacgg atcgggcggt atcggcgatc gccacgctct tgtctccgaa
     7681 gtactccccg ccgaggtcga tgaacttgcc gtccccgacc ggatggccga gcagccgtcc
     7741 gccgacgcgc gtgcgcgcct ccagcacgac gacggttcgg ccggcgcgct tcagctcgag
     7801 cgcggcggtg aggccggcga ggccggcccc gacgaccacg acgtcggcct cgagcgtctg
     7861 gccgccgttg cgcatgcgcc gctcgctccg ccgtccgtca gtcgacggcg gccgcgaacg
     7921 ggcgcgccgc ggcggccacg cggtcgcccg tcccctggga gcgagggcgc gcgtccggcg
     7981 gctccggggg ctcgaagtcc ggatgcgtgt tgatccatcg ccgccgcccc tcgcgcacga
     8041 gcaggcgccc agcctcgcgt acgaccgcgg cgcggtcgaa gcgccccttc accagcacgc
     8101 gtccggtcgc ctccgccaag cgtgggatgg tgaggatgtc gtagtagcac gagcggtggc
     8161 tgaacaggtc gaagaagcga tcgacctcac cgcggccctc gagcgcctgc acgagctggg
     8221 gcgccatcgt ggggatcgcg ccggcgcagc cgaggtcgtt ggtcatccag tagtggccgg
     8281 cgaacttgcg gtccctccac gcggcccact cggcgagcgc cagatcgagc ggcggccccg
     8341 tcccgtcgag gcccgcgacg atcgtgggcg cgagccgctc ggcctggatg aacgcgtcgc
     8401 cgatgccgcg gccggcggcg gggtccttga agtgccccgc gtcgccgacc agcacccatc
     8461 cgggtcccgt cggctcgcgg aagtacccct cgaaccggac tatgccgaag atcttggtgg
     8521 ccctccgtgc gtcggccagc gccgccgcgg tcggctcgca cgcgcgcatg tgggccatca
     8581 ggttggtctc cgggtcgcgg cggaagtcct cgcgctgatg gtcctcgggc gagactccca
     8641 cgaggtagag accgctgtcg gcggcgcccg cccagaccat ccggtcgtcc cagcgatgga
     8701 agacgaacag gtcgtcggag tccggtgagg cgccctcgaa gaacgtgaag tagtacgagc
     8761 gctcgttgcg cgtgacgttg tacctgcgcg ccccgcacag cctggccaca gtcgacttgc
     8821 ggccgtcggc gccgacgacg agcggcgcgt ggagctccga ctcgcgaccg tcgtggacca
     8881 cgcgcacgcc cgtgacccgc ccggcctcct cgagcaggcc cacgaccttc gtgtccatcc
     8941 gcatctcggc gccggcctcg gcggcggcgt cggccaggat cgagtcgagg aggtgccggc
     9001 gcaggaacgc ggcgccgccc aggtcgcccg gtcgcagcgg gaacctggtc acgagccgga
     9061 gctcgttgag gcgggtgtcg acgccgtgca tgaagcggac gccggtcttc cgaaccgggt
     9121 cgagtatccc gaggcgcttg aagaagttga gggagtcggc ctcgatcagg tgagaggaca
     9181 ggaccgtgcg agggaacgtg acctgctcga cgaccgcgac gcgcaggccc gagcgggcca
     9241 gcagcgtggc gagtggcgag cccgcgcagc gcgcgcccac gatgaccacg tcgaaccgct
     9301 ctcccggcat cggcgcgaag ctactcgcgc agggagcccc accgcacggc cgccgaagcg
     9361 ttttcaccca ggggtctgcg gtatgccacc caggcgatgg aggcgcgcgc gacggccgtc
     9421 ggcggagacg cggtcgttcc tagtatccgt ccatggcgag agccgcggtg cccccgggcc
     9481 cacacgtcgc gctcgtgcag gtcgcgcggt tcagccgcaa cccactgcgg ttcctggaga
     9541 gcatgcggcg ccgctacggc gacgtcttct cggttccgta tccgatgttc ggacgcccgg
     9601 tctacatggc cgatcccgcc ggcgtgcaga ccatgctggc cggcgacccg gcggtcttcc
     9661 acgcgggcga ggcgaacgcc ggcccgctcg ggcccgtcat gggcgcgcga tcggtcttcc
     9721 tggtggacgg ggaggatcac atgcgcaagc gcaagctgct cctaccgccg ttccacggtc
     9781 gccggctccg tcaatacggc cagacgatcg ccgagctcgc ggcgcgtgag gtgcggggct
     9841 ggccgcgggg cgagcgcttc gcgctgcggc cgcgcatgca ggcgctgacg ctcgacgtga
     9901 tcgtgcggat cgtgttcggc gtgaccgatc ccgaacggct cgcgctctat cgccagcgcg
     9961 tcccgcggct cggcgaggtc gccgaccccg tcatgtggat acccgtggcc cgccgcgacc
    10021 tcgggcgctt cagcccgtgg gctcgcttcg tgcgcgcccg cgacgccgtg gacgagctga
    10081 tctacgacga gatcgagcgc acacgccgcg acccgcgcct ggaggagcgc gacgacatcc
    10141 tggcggtgct cctccaggcg cggcacgacg acgggagcgc catgagtcgc gaggagcttc
    10201 gcgaccagct catgacggtg atcgccgccg gccacgagac gaccgcgacg ggcctctgct
    10261 ggctgttcga gcgcgtgctg cggcacgccg aggtcgagga gcgcctgcgg gccgagctcg
    10321 cggcgggcga cgacgcctac ctcgaggcga ccgccaccga gacgttgcgc gtccgtccgg
    10381 tggtgaccga catcgcgcgg cgggtcaagc gcgacgtcga ggtcgcgggc tggaccctgc
    10441 ccgccggaac cttcgcgatc ccgtcgatcg ccctgatcca cacgcgcgag gacctgtacc
    10501 cagaacccca cgccttccgc ccggagcgct tcctcgacac gaagccgggc acctacacct
    10561 ggatcccgtt tggcggcggg gcgcgccgtt gcatcggcgc cgccttcgcg atgctggagc
    10621 tcaagacgat cgcccgcacg atcctcgacc gcacgcgcct gcgtgcgccg gacccgcgac
    10681 cggagtctcc gcgcgcgcgg aacgtgacga tcgtgccgtc gcgcggggcg gaggtcgtga
    10741 tggaggccag ctcccccggg tgaaaccagc cacccagttg gacggaccgg cggcgtgcga
    10801 gcgccccgag ggcgcccgca cgtcgaccat gtggcccacg tgctcgagtc gctgacaccc
    10861 acgtcctggc cggcggaggt cgacgacgtc gtcgccggag acctcagcgt gatgttcgcg
    10921 acggtgactc ccgcgcgcgg cgtcgtcctc acgccgttcg cgaacatcgc actggtcgac
    10981 cgcggggccg ggacggtcac catcaccacc tcgctcggcc tgtggggcaa gctcgagcgc
    11041 atccagcgca atccgcgggt cgcgctcgcc ttccacacgc gcgcgaacgg agccagcgaa
    11101 cgcccggagt acgtgctgct ccagggcgag gcgtccttct cgtgggtgcc cgatcgttcc
    11161 tggctcgaat cgatcggcga caactgggag agccacctcg acacccggcg gcagggacgc
    11221 gcctgggact ggtggctctc ggtgtaccac tgggagcggg tggggatcga gatcaaggtc
    11281 gcgcggaccg tcgtgtggcc cgatctggac tgcgcgggtc cgcccgccgt gcacgggccg
    11341 gagctcccgg gcccggcgcc ggcgcagcgc ccacccgccc ggggcacggg ttcccggatc
    11401 aaccacgcgc gggcggccgc gcgcatccgg cggctacctc acacgctgct cggctgggtc
    11461 ggcgcggacg gcctgcccat ggtggtcccg gtggggctgc gcggggcgaa tccgctcggc
    11521 atcctgctgg aggcgccgcc ggacgtcgtg ccgccgggcg gccggcgcgc cgggctcacc
    11581 gcccactggt gcggggctcg ggtgatcggt cacgagcagc gcgcgcacac cggctggctc
    11641 gagccggaga ccgaacggct gctcctctac gcgccgcaca cgaagaacgg gtaccggctc
    11701 ccgccgtcca cgcccctggt ccgagcggcg acgggcttcg ccacccgccg cgggttccgc
    11761 gcgggacgcc gcgccggcgt gctgacggcg cagccgcgat gacctagcgg ctcggccgtg
    11821 actactgccc gcggttgcgc cgcccgggca gccaccagtt ggcgcgcccc agcagcgcca
    11881 tggtcgccgg cacgaggaac gtcctcacga tggtgatgtc gatcagcacc gcgatcgcca
    11941 tgccgaggcc gagctgctgg agcggttcca tccgcgagct ggcctgcgcg aggaacacgg
    12001 ccaccatcac gagacccgcc agcgtgatcg cgcgtgcgct cgccacgagg cccctctcga
    12061 tcgagcggtc ggacgacgct ccggacaggt actcctcgcg tatccgcgac agcaggaaca
    12121 cctcgtagtc ggccgagagc ccgaagatga tcgcgaacag gaacagcggg acgaagaagg
    12181 agagccggtc cgattctccg atcccgaggt cgtggtagag cagcgtgagg atgccgagtg
    12241 cggcgcccac cgacagcagg gtcgtgaggg cggccttgat cggtacgacg atcgaccgga
    12301 aggcgaacag cagcaacgtg gcggacagca ggagcgcggt ggcgacgacc gccggcgtac
    12361 ggtcgtgcag ccggtcgagc aggtccaccg ccttcgcggt cgtcccgccg acggccgcgg
    12421 ccgcgggggc gtctccctcg gtcagcttgc gtcgcaggcg ccgtacggtc tcctgcgccg
    12481 ctgggctgtc cccgttgtcg agcagcggcg cgagcaggag cagccagccg tccttgccgg
    12541 gccggatcac gatcgggcgg ccgaccccgg ggtcgcgttc cagcgcctgg gccacccgct
    12601 tccggttccc ccgcgccacc acctgcaccg gcgtcacggc gccgccgccg aagacgcggc
    12661 ccacgcgctc gctgaccacc cggaccctgt cgttcttcgg cagggccgat gccgtcgggc
    12721 tggcgagcgg ggcgtgcagg agcgggatcg tgagcagcgc cagcgccgtg gccgcgccca
    12781 cggcgacggg caggcgccgc cgcaggacga acgccgccag ccgtttccac gcgcggccgg
    12841 actcggccgc ggtggccacc cgccggagag ccaggcgatc gaggtgccgg ccggccagga
    12901 cgagcacggc cgggacgagc gtgacgcctc cgagcgcggc cacgagcgcg gccgcgatca
    12961 cgccgatcgc catcccggag aagacgccga cctggagaag gaacagcgag gcggtcgcgg
    13021 tgatgatcgt gaggcccgag aagacgaccg cgcggccggt ggtcgagacg gtctccctga
    13081 ccgcatccgg gatgcttccc ccgttctcgc gcagcgcctc ccggtagcgg gtcacgttga
    13141 agagcgagta gtcgatgctg agcccgaggc ccagccccag cgccgtgttg ctggcgaaga
    13201 cgctcagcgt cgtcagctgc gagatcgcga acagcaggcc gaacgtgacg gcgaggacca
    13261 gtcccgcgag caccgtcgga acacctgtcg cgactatcga caggaaggcg acgaggatga
    13321 tcgccgaggt gaccggaaac gagatcttct cgaccctgtc gaggtcgtcg cgcgcgatgg
    13381 tcccatagcg gtcgtagtcc gcggcctcgc cgacgatcgt cacgtcggcc tggccggccg
    13441 cccgtttggc cgccgtccgg acgtccgcga gctgatcctg cgcatccacc agcgtcgacc
    13501 cgaggtcgaa ggcgaagatt gcgaagttgg ctctgctcac gaccgccccc tggcccacct
    13561 cggcgacgtg cggagcggcg gcgacggcct tgcgcacccg tgggaggagc ggcgcgagcg
    13621 acgacgcgcg cccctcggga accgacacca cgacgaacac gtgcgtccgg tcctcgccga
    13681 agatgcggtc cgcctcgagt gcgtcggtgc gggcggactg ggagccgggg acgtcggtgc
    13741 ccccgttcgc cagccgcccc ccggccgcgg ccacgacgcc gcccaccacc gccagcgcga
    13801 cccacgcgaa cacgatcgcg cgcggatggc ggacgaccgc ggcgcccagc cgggcgagca
    13861 ggccgcccgg ctcgcgtctt tggctatgcg tatgaatggc ggctttttac cagctcccgc
    13921 gccatccacc ggcgcgggag cgtcgcgaga gccgggggag tcgagcgacg gatcagcggc
    13981 ggaggctcga gaccagcttc gtccgcacgt tgagcatcgc cgtcatgagg cggtcggcac
    14041 gcgacccaag cgggggcagc gcccccacct ggcgcgcgga ctcgagctgg tcgaacacga
    14101 tggtgttggc cgcgatcttg ccgtcgcgca ccacgaggtg gtccatgccg tcgagcgcga
    14161 tcgacctgcc ggtgccgtcg agcccggtca accggccggt gtggcgaccg gccatgtgcc
    14221 agtgcacgaa tacgtccgga ccctccgcgg cgatcgcgat cacatcgaag cggacgtcgg
    14281 gcatcgccgc gaagagctcc tcgaaccagg ccgccacttg gcgtgggccg cggatggtct
    14341 gttcgggcac ccgccacagc gcgtcctccg accacagctc gctggccgcg cgggcgtcgt
    14401 gccggttgac cgcctcgaag gcgcggcgga cgaggtcggc gttcgtgctc gtcgccgggg
    14461 aggcttcgcg ggtcggcatg ggcgcgattc tcacccgggg tgggcgtggc acccgcgacc
    14521 gggaggcgcc ctctccatcc gctggggggg aaagcgcagc cgcctgggtg gggcgcacgc
    14581 cggcaccgag cgaacgtttg cgctccccgc cgccgggtac ccgaaccgca tgaccgacga
    14641 cccgcaggaa aagcagccgc agcccgtcga ggacggcact cccgaggacg ctcccgagta
    14701 cgcgcccgcg gagaaggaca agtcgatcgc ggacggctcg cacagcgccg ccgccatcgg
    14761 cgaggccggt cctgccggcg agcgcgtaga cggctagcgc cgctgcgccc gcggtccgcg
    14821 ccgctgcgcc cgcggtccgc gccgctgcgc ccggcggccc gccgccgccg ccctgcccgg
    14881 cggcccgctc cgccgccgcc ccgcgcccct gatataagcc gccgggtggc ggtgatggaa
    14941 gctccggcga tccgcgagct ggccgacgag ttgctcgcgc ggtggaacgc gcacgacgtc
    15001 gacggcgtgg ccgcgctctg ctccgaggac gtcgtcttcg tcgatcccgc gcttccgcag
    15061 ccggcgatcg ggcgccgcgc catgcgggac acggtcgcct cggccctggt cgcgttcccg
    15121 gatttccgcc tcgagcggat cggcgacccg ctgatcgcgg ccgacgaggg tctggtgctg
    15181 gtccgcttcc ggatgatcgg gacgatgaag ggccactggg gggccgcgaa cgtcgccgcc
    15241 accggccgct cgctggacat ccgcggcatc gacgagtggc tcgtgagcga cggtctgctc
    15301 cgctcctgcg tggcccacta cgactcgacc gaggcggcgc gccagctcgg gatgctcccg
    15361 cccgagacgg gcctcatgaa ccgcgcgatg ctgcgcatgc agcacgtgca ggcgtggttc
    15421 cagcggcgct cttcgcgcta gcccggctca gacccaggag cgcaggaaca ccgcgaccgc
    15481 gcgcggccag gcgctcggcg tggcgaggat cgggcgcacg agcccccacc tgctcatcgt
    15541 cacgtggcgc tcgctcacga ggccgccgga gagcacgagg cggtcgcggg ccttgacccg
    15601 cagccgccgc cgtccgaccg tccccgttat ctcgaagctc acccacaccg cgtcgccggc
    15661 gctctcccag ccgaccacgc gcaggcgcag gtcgccgaag atccgcgcca gcccgtgcat
    15721 gttgcgctcg aagctcctga agccgcgccc ggtcctcacg agcggctggc tgagcacggc
    15781 gtccgggtgg agcagggggc gcgtggcggc gagcgcctcg tcgagtgtcg ggcggctcca
    15841 cgcccagcgg agcgtctcgg cgagcgcggc gtggtcgtct ctcgcgccgc ccgcgacgcg
    15901 cgcgcctccg gtagcgggtt gcgcgcccgc ccgcccggta gcgagctgcg cgctcgcccg
    15961 ccgggcggcg agccgcgcgc ccacccgccg cgcggccgcc gctcccgaca gcgccgccgc
    16021 ctcgatcagc ggcccgtaca ggtagtcgcc cgcgagctgc acgggaccgt cgatcccgtc
    16081 gcgcatccgc gcgagctcgc gcgcgcgccc gcgcgggaac agcgggacct tctcctccca
    16141 gcggacggtg gcccggcccg tcacgtccgc cgccagctcg gggtggaggc gctcgagctg
    16201 ctcctgcagc gcgccggcca gctcctcgtc gtcggggatg ttcgcgacgg cgtccgggtg
    16261 ggcggcgacg aacacgagcc ccgcgtgcgc cgccgggtcc gtggccgcga acgagatgtg
    16321 cgagatgggc ccccgcgccg agccgccggg gaatacctcc atgtaaacgg cgcgcccgtc
    16381 cggcgcggcg cgcgtgtggt ggccggtggt cttgaagtag gcgtggaagt gacgcgagta
    16441 gcgcgtggcg gcgagcagct cgcgcgcggg tccctcggcg ccgggccacg cgcgcagcgc
    16501 cgccggcgcg gtggtggcga gcaccacggc gtcggcctcg aagccgtcgc cgccggtgtg
    16561 cacgagcacg cgctcgccct cgcgctccac gcgcgtgacc tcgcagccga ggcgcacggg
    16621 gaggtcgcgg gcgagcgccg ggccgagggt gccgagcccg ccgtcgagcg acagcgggcg
    16681 accgagcggg aagcgagcgc cgagcgtcaa cagcggcagc gagacctggt cgatcgagta
    16741 cccgcagagt gcctgcgaga gcggggcgaa cacgcgctcg acggcgtcgg tgccgagcag
    16801 ccgccgcggt gcgacgcggt ccacggcggc gacgctggcc atgtcgaaga agcgggcgct
    16861 cggtgccgag cgcaggagcg gcagcgccat aagcccgagc cgcgcgcgct gcgccggcga
    16921 cagcgccgag tagcgcgcca cctcgagcgg gcgcccgaag tcgaggtagt ggaagcgccc
    16981 gccgacgaac acggcggtgc agaggctcgg tccgatcggg cgcagcgcgg actcgagccc
    17041 ggcccgctcg atcaggcgca gcacggtccg gtagcggggc gagacgaagt gcgcgccgag
    17101 atcgaccgcc agcccgtcga gctccgccgt gcgcacgcgc ccgcccgcgc gatcgccgcg
    17161 ctcgagcacg accacctcgt ggccgcccgc gcgcaggtcg agcgcggcgg tcagcccggc
    17221 caggccggcg ccgaccacca cgacctcggt gcgctcagcg ctcatcgagt gcgcgtcgca
    17281 gcggaagccg aaggctcgcg cgcacggcca gcagcgtggc aacgccagcg atgacagcga
    17341 ccagcaccac ggggcgcacg aactgcggcc acgagatgta gaggtcgacg ggcagcgaca
    17401 ccgacgtggc gaggtgatgc accgcgagga cccgtcccac gatcccgagc acgaggccca
    17461 cgagcgcgcc gatcagcacg atgcaggcca gctccgcgaa cgtggcgctc gccacctgcc
    17521 gcgacgacat gccgatcgtg cgcagcgagg tgatccgccg cgctcgcccg agcaccccga
    17581 gcatcgcggc ggccgcgacg gcgagcacgc cggccagcgc gatcagtgac ccgatccggc
    17641 tgaccgtggc gagctgctcc tccgcgttcg acttcgcctg cgcgcgcatc gccggcgacg
    17701 tggacgccgt caggccgcgg cggcgcagca ccggctcgag cccgcgcgcg agctcgcccg
    17761 gaccgactcc gggctccggc tggagcttca acgcggtcac gaagtcgcgc ccccaccacc
    17821 tggcgaggct cgcggggttg aagacgatcg ccccggcggc ccagccatag ttgctgatgt
    17881 tcgcgaccac gcggacgtgg cgcggaccga ccggcgtcgg gacggtgagc cgcgatccgg
    17941 gtcgcggcga ggcgccgccg ccgaggtcgc tgctcaccgc aacggcggcc gggtcggtgg
    18001 tgagcgcctt cgccgcgcgg cggccgtcgc cgcgggtgaa gccgctcggc gagtcgcgct
    18061 gatcggggtc gaagccgaag gcgaggatcc ggcgcccgtt ccacgagatg aacgacgcac
    18121 gccattcgga tatcgagccg accttcggcc tgcgctccag cgtgctccgc aggcgcgggt
    18181 cgatcggctg ggtcaggaac acgttgccct cggagtccgg gcggacgaac accgcggcgc
    18241 ccttgtaggt cgagccgccc agccggccga cgccggtgct caggtcgctc gcggtccccg
    18301 cgatgatcgt ggtgccgcac accgcgagcg tgaccactgc caccagcgcc gaggcgcgcg
    18361 acgggaacgc cacgatctcc gtgatgccga ccagcggggc gccgcccggc gagcggaggc
    18421 gcgcgcggat ggcccgcgcg acgaccggga ccgcggcggg gatggcgagc aggccggcga
    18481 acacgagcag cccgacgccg acgatcgcct gccccgccag gctgagcgcg gccccagcga
    18541 gggcgaggac gatcggggca gccacgagcc agacgctgcc tgtgagcctg cctgggctgg
    18601 cggcggcggt gccctcgccg accagcgcct cggccggcgg cacgcgcacc agctcgcgcg
    18661 cgggcacggc ggccgcggcc agggtcgcgg ctaccccagc cgcgacgctg agcgccacga
    18721 ccccgggcgt catccgcgcg gtgcgctcca cgatgaacac cgacgcgagc agcgggtccg
    18781 gcgcgggcag gagcccgccg agcgccgcac ggccgagcgc cacgccgagc acgctgcccg
    18841 cgagcccgac gaggagcgcc tccgcgagca ccgccaccag gagcgcgcgg gacgagcagc
    18901 caagcgcgtt gagcgtggcc acctcgcggc ggcgctcggc cgcgatcagg ctcatcgacg
    18961 cgtacgccag cagcgcgccg accacgaggc tgagcgctgc gaacagcgag gacgagttgc
    19021 ggatgagcga cgacgcctgg gcctgcgccg aggcgtcgaa gtccgcgccc cgcacgtccg
    19081 cgacgcgatc gccgatcagg cgccgcagct ccgcgcgggt gtcgtcggcc gagccgcgcg
    19141 gcacgaccgc tatccgctgg atgcggcccg ggttgccgtc gagccgctgc gcgagggcaa
    19201 gcggagcggc gacgatcggg gcgtcggcga cgtcgccgag gcgctcgtcg gggagcacgg
    19261 cgccgacgcg cgcgcggcgg atgccgacgc gcgagcggac cgtgacccgg tcgccggcgc
    19321 cgacaccgag ccgcgtcgcg atcgcgcgcg acacgacgag cccgcctccg gcggagccgc
    19381 caccgcgacg ctccgcgccg gcccgcgcga acgtcgccgg cgccccgccc agctgctccg
    19441 cgtcgctggt ggctccgatc agccacaggt cgaaccgacc gcggccggtc tccaccccga
    19501 ccaggtcgtg gaccgtcggg accgcgagcg cgaccgagcg tgagcggagc acgcgtcgct
    19561 cgaccgagac cggcatcccc agcgggctcc gcgccaccac ctccacgcgc gcgcgacccg
    19621 cgaggcgctt gtacgcgtcc tccgcccccc gcgtcgtcga gccgttctgg ctgagcaccg
    19681 ccgacagcag cccgacgccg accgcgacgc ccagcacggc cagcagcaac cgcacggccc
    19741 gccgcagcgg ccgggcgagc cacgcctgag ttgcgccgcg ggccccgggc acgccgatca
    19801 ggcctgcgcc gtccgggcga cctcgccgct gtcggagacc ctcccgtccg ccatcgtcat
    19861 cgtccgctcg acgtagtcgt gcacgaccgc gtcgtgggtc acgatcagca ccgcggcgcg
    19921 ctgctcgcgg gcggcctcca ccagcacctc gaccacgctc gcgccggcgc ggcggtcgag
    19981 gttgcccgtg ggctcgtcgg cgagcaccag gcgcgggcgg ttcacgagcg cgcgcgccac
    20041 cgccacccgc tgctgctcgc ctccggacag ggcggagggg aggtggtcgg cgcggccctc
    20101 gaggccgacg cgcgccaaga gcgcacgcgc gcgctcggcg gacttggcgc ggctcacgtt
    20161 gtcgagcctc gctaccagag ccacgttctc gagtgccgtc agccccggca tcaggttgaa
    20221 gaactggaag acgaagccga tgcgctgacg gcgcaggcgc gacaggccgg cggcgtccag
    20281 gccggcgagg tcggtctcgc cgtggaagac gcgccccgac gacggcaggt ccatcgcccc
    20341 ggcgatctga agcagcgtcg tcttgccgga gccgctcggc cccatcaccg cggtcacctc
    20401 gcccgggtag atcgacatcg acacgtccgc gagcgcgtgg acctcgttcg ctccctggcg
    20461 gaacacgcgc gtgacgtggt cgaggcgaag cgcgggcgcc tcgcccgcgg ggcgctccgc
    20521 gctgacgacg ctcatgccac gggttgaggc agctcgcgcg cggcgcccgc gttgcggatc
    20581 cgggtgaccg ccaggatcgc gcgcgggacg gcgagctgcc cgcccacgtg cagcggaccc
    20641 gcgttgcggg tcacgagcgc attcagccgc tccgccaccg agcggtcgtg cacgccgccc
    20701 gcgaacatca gccgctcgat ggggtagagc tcgcggccgg tggcgtagct ctcgatcagc
    20761 agcgcgtggg gcagcagcgc ccgccgccgc cgcgcgcggt agcgggcgag cccggccctc
    20821 agcggctccg ccccgttcag cgccggcccg acgcagccgg cgagccagtc gccgctctgg
    20881 acggcccagc cgcacccgac gccccagagc gggtcggcgg ccagcgccgc gtcgcccgcg
    20941 agcgcgacgc cggagcccac gggcgcgcgc cggatgctcg tcatgtcgat ccgcccgacg
    21001 acgtcggaga cgcgctccga gtcgcggatc ggcggcgggt cggcgcgcga ggcgatgaag
    21061 tcctccagcg cacccgccgg gtcggcccgg aacggtgcgc ggcgctcgag cgagtccatg
    21121 gcgacgtaca gcgtcaggtc gctgtcggtc gggaacgcca tgccccacgg gaccccaccg
    21181 caccagaccg acgcgtccgg gctgccggcg ggcttcggcc ccgcgtagta ggcgctgaag
    21241 gcgaagcgcc cgtgacggcg ccggtgcgtc ctcagcccgg cgagccgcgc gaccgccgag
    21301 tcgcgcccgt ccgcccctac gacgaggcgc gagcgcagcg tgcgctcgct cccgtcgcgc
    21361 gccgtgatcc gcacgcccgc gaccgtgccc gactggtgca cgagcccggt cacccgctgc
    21421 ccgagcagca gctcgacgcc gggggccgcg gcggcgcgct cgcgcagcat cggatcgagg
    21481 tgctcgcggc gaaggttgat ccccgcgggc gcactcgtgg gcggcggcgg ctcgatccag
    21541 ccccacggcg tgcgcacgcg gaaccgcgag cgaaccgcgc ccgcctcgag catcgcgtcc
    21601 accaccccga ggcgctccag cgctggcatc gcggaggact gcacgaagtg accgcagacc
    21661 cgcttgtacg cgctcgcgtc cggctggcgc tcgaccaggg cgaccctcag tccctgctcg
    21721 gcgaggagct tcgtggcggt gctgcccgcg aggcttgcgc cgacgacgac ggcgtcgaac
    21781 gtgggggcgg aacgcgtgcg ggaggtagcc gcttggcgat ccttcacggg cggtcgagta
    21841 tatccctcgt acatccccgt gggcatgtgc gcttagcgct aaatcgcgcg ggtagactgc
    21901 ggcgcctggc tcccacggcg accgctcacg cgacggtgaa ggaccggatc tccgggctgg
    21961 tgcggctgct ccgcccgtac agcatcacgt ggaccgcgtc gtccgtcgtc gtgatggtga
    22021 tcctgctcac cggcgggcgc ttctccacga tggacctggt cggctgcctg ttcgcgatga
    22081 gcgcgatcgg cggcgcggcg cggacgctca acgacgtggc cgaccgggcc accgacgagc
    22141 tgagcacgga ggccgaccgg cgcggccggc cgctcgtgac cggcgtggtc tccgcgcgca
    22201 tggcgctcgc gcaggtagtg ctgctcggcg gcgcgggcat cgcggtcgcg tttgccgtcg
    22261 acctcggctt cgggatcttc atggggctgg gggcgatggg gctggtgctc tactccgccg
    22321 gcccggcgcc cgtcgcgggg ctgcccctct cgcagggctt ctggatcgcg ttctggacca
    22381 cggtctacct ctccctgtac ctcgcgctcg acggggacgt cggccggggc gtgccgtacc
    22441 tgatctcgac ctgcctgttc atggggatcg gcgagacgat cgcgaaggac ctccgcgacc
    22501 tggagaacga cgcgcagggc gggcggcgca ccacgccgct gctgctcggc ccgcgccgct
    22561 cggtgtacgc ctgcttcctc gcgtacgccg ccggcggcgc cgggttcgtg gtcaccgccg
    22621 cgctgcgctc gcccgacgtg cggctgccga tcgcggtcgg ctgcgtcgcg ctgctgtggg
    22681 tcgcgcgctg cgtgcagtcg ctgaacgcga cccgggacgg gtacgtgaag gaggaggccc
    22741 gggtgctcca cgtcggatcg gtgcgggtct tcctggtcgt gaacctgctg ctgctggcgg
    22801 ggatccccct tgccgactga gccggcgagc gtcgcggccc ccgagcggct gtggccggtc
    22861 gcggagctcg agcccgaccg cgtcggcgac gcgctcaggc ggcttctgaa cgaggcgggc
    22921 cccggcgcgc tgccgcggac cgcggacgac gtcctggcga gccaccgctg cgcggaccgg
    22981 ctcgcggcgg ccgggctcac ggaggcggac gggacgccgc gcttccgcgt gaacgcgctc
    23041 gccggaagga tcttcgtgac cgatctgccg gaggccaccc gcgccggcgg cgcgttcccc
    23101 tacgcggacg aggccgagcg gctgctcgcc tacctggagc gctccggcgt gagcacggcg
    23161 ggcgcgcacc tcgtcgacgt gggcgcgggg tgcgggcacg tgacgctctc ggtcgccgcc
    23221 gcgcggcgca ccgccgtcga ggtcaatcca cgcgcgcgcg agctgctcgc cctcaacgcg
    23281 cggctgaacg cgctcgaggc cgaggtggtc tccggccccg cgcagtcgca gctcgcgggc
    23341 ctcgatccga tcgtctcgcc cgcgggcgag accgtcgtgg tcgcgatcct gccccacgcc
    23401 ccggcgcccg acagctcgat gctcgccggg ttcgcgaacg gcggtcgtac cggcgcggcg
    23461 gtgatcgagg ccgtgctgga ggagctggcc gctgtcgccg cgcgcgcggt ccgcgtcgtg
    23521 atgctctgct acagcctgag gtcgtcggag accgaggcca cgctggtccc gcgcgccgcc
    23581 cgccggctga tgccggaccg caacgtcgag tggacgctgc tcgccggcgc tccgatgtgg
    23641 cggatcgacg gcgtgatgag cgagcgctcg ccgatggcgc tggtcccggc gctggctcgc
    23701 aaggcgcagt gccggttcta ctgcgacgcc gaccagcgcg agagcacggc cgcggcgtac
    23761 tcggagctcg cgaccgagct ggccgccgac ggctgggacc agctctgtta cggcgccctc
    23821 gacgtgcgct agccagcggg cacggcggcg tccacggcgc gcagcgacag ctccggctcg
    23881 aacccgtcgg cgccgcggcg gaccacggag tgtgcgagcc agcgctcgtc gtcgcgcatg
    23941 gggtggtcgg tgcgctggtg cgcgccgcgg ctctcctcgc gggcgagcgc gctggcgacg
    24001 atcgccgcgg cgatcgcggc ggccggctgg cgatcgcgct cccagctcgc gcggagggtc
    24061 gcgagcccct cggcgagcgc gtcgccgtcg cgccgtatgc cggcgcagcg gccgaggagc
    24121 gcgcgcagct cgggcccgac gcggtagtcg ggggcgcgtt ccggccgggg cgtgtcgccg
    24181 ggcggggccg cgccggaggc agccggagcc ccctcggtcg ccgggttgcc gggcggagcc
    24241 gccgcggcgg tcgcgccgga gccggccgga gccgctgcag ccgtgtcgcc ggccgtcgcc
    24301 gcggcggcga cgcccgcccg cgtcccgaac accgtcgcgt cgagcagcga gtttccgccg
    24361 agccggttgg caccgtggac cgagacgcac gccgcctccc cggcggccca cagtcccggc
    24421 acgttcgtgc gcccgtcgag gcccaccgcc accccgccca tcgtgtagtg cacggcgggc
    24481 tcgacccgga cgggccgctc acgcacgtcg acccccacgg cccgcgccag ctcgcggcgc
    24541 acgaactcga tgtgctgcca gttctccgcg ggcacggcgg cgaagtcgag cagcacgccg
    24601 ccctcgggac agccgcgccc cgcgcgcagc tcctcgtcga tcgcctggct caccagcgcg
    24661 cgcggcgcga gctcgcaacg ctcggggtcg tagcgctcca tgaaccgctc gccgaggccg
    24721 ttgagcagcc gcgcgcccgc gccgcgcgct cgctcggaga cgagcaccgg gctcccggcc
    24781 gcgaccgtcg ggtggaactg gacgaactcc atgtccatca gctcggcgcc ggcggcgtac
    24841 gcgagcccga gcccgctgcc gtcggcctcg gccgcggcga tcgagggcga cagcagcgcg
    24901 ccgtagcctc cggtcgcgag cacaaccgcg gaggcgcgga cgtgaagctc gtcgcgcccg
    24961 ctcgccagcc acgcgccgcg gcagccgccg ccgctgacgg cgagcgcccg cgcgcggacc
    25021 ccccaacgca gctgggcgcc cgagcgttcg agcgccccag ccagcgcctc cacgatcgtc
    25081 agaccggtga tgtccgggag cgtgtactgc cagtgcgccg tgcgcccgag tagctcgcgg
    25141 cgcatctcga cgccgtcgct cagcaggccg gcgacgacct cgctcgcgct cgcgcacatc
    25201 cgctcgacgg cggcgcggtc ggccagcccg tcgcccacgc gcagcgtgtc gtgcacgtgc
    25261 tcgcgccagt ccgcctcgcg gtcgaacacg ccgttcatcc cgcccaccgc cttcaccgag
    25321 tgcgactcgc ccggcgtgcc ggcggcggcc accatgacct cggccccggc ccgcgtggcc
    25381 gctagcgccg ccgccagccc cgccgccccg gcgccgatca ccaggacgtc gaccgtccgc
    25441 tcgctcgtgg ccaagcgccc ctactgctcg ggcttgcgcg cggcgaagca gagcagcgag
    25501 cccgtcttcg actcctgcca gtccgcggcc gcgtgcgcgt ggaacaccga gcgcagccgc
    25561 gccgcactga tcggctcggc catcaggctg aagcggtggc agatccggag gctgagcgag
    25621 ccgcgcaggt tctcgcggtc caccagcgcg tagcccgcga tcgatccgcc gggacgcagc
    25681 acgcgcatga actccgacac cgccgcctcg cggtcgggga agtggtggaa gccgccctcg
    25741 gtgagcacgt agtcaacgct cgcgtcgggc agcgggatcg aggcgacgtc cgccgcgacg
    25801 aagtcgccat taccgctgaa gcccgcgcgc tcggcggacc ggcgcgctcg ccgcaccatc
    25861 ccgtccgaga ggtcgaccgc gatcaggcgg ctcggcttca ggcggtctgc gtagtggggg
    25921 aacatgcggc ccatgccgac cggaaggtcg aggatcacgc ggccgctctc ccagctctgg
    25981 gtgaagcgct cgacctcacg ctcgcccttg cgcaggtcgc tgccccaggg gatcgccccg
    26041 tacaggaccc acacgggcgc gatccggtgg ttgagcgtcc agagcgctcc gccgacgccg
    26101 tcgtaggcgg ctttcgtccg gctgacgatc gtcggttcgg cgatggtttg cactgttgtg
    26161 gaggtcaatc gcgaaagggg cgctgaacgg taagcgccgg tatgctaccc cgccccgatg
    26221 gcgccacttc cacggtgatc cgacgcaccc gttgagcgcc gagcgttcag gcgccctgag
    26281 tcctctgctc gagcccgtgg cgctcatcgg cgaccgccct gagggcgaag ccgagtggcc
    26341 gctcgccgac gcctgcggcc acgcggtgac gcacaccggc aagcagctgc gcccgcggct
    26401 cgtcgcggag gcgagccggc tcggcagccc gccggacgag gaggtcgtga aggcgatgtg
    26461 cgcggtggag ctgctgcacg tggccacgct gtcgcacgac gacgtcgtgg acgcgacccc
    26521 gctgcggcgt ggcgcgccct ccgtcgaagg ccggttcggg gcgttcgccg ccgagtacgc
    26581 cggcggctgg atgctcggga gggcgctcga gctggccgcg gagctgggcg agccggcgct
    26641 agcgcagttc tccgacgcgg tgtgcgagct gtgcgagggc gagatgctcg agacgcagga
    26701 gctgcgcaac cccgggcgca gcgaggagag ctacctcgcg gcgatcgagg gcaagacggc
    26761 gagcctgttc cgcctgtcgg cgctgctcgg cggcacgctc gccggcgtcg atgcccgcgc
    26821 gctcggcgcg ctgcgcgagt acggccactg gctggggatg gcgttccagc tcgcggacga
    26881 cctcggcgac ctgctcgccg acgccgccgc caccggcaag gagcgcggca ccgacctgcg
    26941 ccagggcgtc ttcacgctgc cggtcgtata cgcgctcggc tccgatcccg agctggccgc
    27001 gctcctttgc gacgagatcg acgcggccgc cgtcgacgcg atcgtcgatc ggatc
//
LOCUS       DASVPL010000163         4345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_100029, whole genome shotgun sequence.
ACCESSION   DASVPL010000163 DASVPL010000000
VERSION     DASVPL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100029"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VF517_08405"
     CDS             complement(<1..244)
                     /locus_tag="VF517_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8053001.1"
                     /translation="MTRKREIARADRVLPGVWRLRLPLPWAGVPHVNAWALASGSGVV
                     LVDTGIGGAGGMKQLELAMSQAGFRVEHVRLLVCTHA"
     gene            complement(241..846)
                     /locus_tag="VF517_08410"
     CDS             complement(241..846)
                     /locus_tag="VF517_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX8053002.1"
                     /translation="MILEHRDARPQIADDVYVAPNAVVCGDVQIGSGSRILFGAVVVA
                     EGGPVRIGERCVVMENAVIRGRTGYRATLGDNVLVGPHAHINGAVIGDDAFIATNAAA
                     FPGSRVGARAAIRIGGVLHVNSRLPDDKFVPINWVAVGDPAAILSPDRHDEIWAIQKR
                     LDFPGTVFGVSRAAEGESHMPEAMARYSRWLGAHQDDREIG"
     gene            complement(843..1295)
                     /locus_tag="VF517_08415"
     CDS             complement(843..1295)
                     /locus_tag="VF517_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8053003.1"
                     /translation="MTVHRYATQVAWRGSTAGGYESYSREHTLRVPSAGQELRLSGDA
                     TFGGDPRLLNPEQLIVAAASACQLLEFLAIASKARVDVLEYSDDAEGIMDESDEPARI
                     QRIVLRPRIVVGDGASEQKVLRLVELAHRYCYVANSLRSEIEVEAVIA"
     gene            complement(1292..2905)
                     /locus_tag="VF517_08420"
     CDS             complement(1292..2905)
                     /locus_tag="VF517_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8053004.1"
                     /translation="MAVATRRLKFWGWGYEDQQPSHEQVREAAAGIRAHLGLEPAEPE
                     RPVALSDLDLPAPRLEPPGSLAAICSSDTYERALHSVGRGYRDIVRAMRGQLEHVPDV
                     VARPRDEVEVEAVLSWCADAGAAAIPFGGGTSVVGGVEPRVGDAYAGTVTVDLRALDR
                     VLEVDAASHAARIQAGALGPGLEAQLREHGLTLRHFPQSFEYSTLGGWIVTRAGGHFA
                     TRLTHIDDLVESVRAITPSGTWESRRLPGSGAGPSPDRLMLGSEGILGVVTEAWMRVR
                     PRPLWRSSASVRFETFEAGAAAVRALSQSALDPANCRLLDPGEAALTGAASDGAALLV
                     LGFESADHPLEPWMDRALELCRDHGGSATDGARHRGPGADGGRTEGEGAWRQAFVSAP
                     YLRDTMILAGVFSETFETAVTWDRLEGFVADVRARAEESLREVCGGGTVTCRFTHVYP
                     DGPAPYFTILAPANRGAELEQWDAVKGAASDAVIAGGGTITHHHAVGRDHRPWYDRQR
                     PEAFARALRAAKAELDPGAILNPGVLIDP"
     gene            2972..>4345
                     /locus_tag="VF517_08425"
     CDS             2972..>4345
                     /locus_tag="VF517_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX8053005.1"
                     /translation="MATRTTTVQITLPEMGESVTEGTVLEWLKVEGDRVEADEGVVEV
                     STDKVDAEVPAPASGILKRILVQPDETVPTGTVLGEIEVNGDGGPAAASDGNGGDPES
                     AAAEHPQWGADESAAELVEHGADAEEAHQAAAEAVGSVGDGSAQASDPPPDEPAADGP
                     SELTDVAMPEMGESVSEGTVLEWLVSVGDEVAVDDGLVEISTDKVDAEVPSPVAGVIR
                     EILVQPDETVTNGTVLCRIAPGGGGATPRMPAGETVHRTDDPQPEARPAQTADGGNGS
                     THATPVAARMASAKGLDVSGLEGSGPRGRVTKADVEAALDNGGSAALTGGNGAAPREA
                     ARTPIRGPAATLARFMNESRSIPTATSFRTLEVDVLAARRTALKDAGRKLSFTHLIAW
                     AIVQAARDMPVMTHAYEEVDGKPHRVQPGGVSLGLAVDVERKDGSRSLVVPVLRDADA
                     LDLAAFVE"
BASE COUNT          606 a         1631 c         1600 g          508 t
ORIGIN      
        1 gcgcgtgcgt gcagaccagc agccggacgt gctcgacgcg gaagccggcc tggctcatgg
       61 ccagctcgag ctgcttcatg ccgcccgccc cgccgatgcc cgtgtcgacc agcaccacgc
      121 cgctcccgga cgccagcgcc cacgcgttca cgtgcggcac gcccgcccac gggagcggca
      181 ggcgcaggcg ccacaccccg ggcaggacgc ggtccgcccg ggcgatctcg cgcttgcggg
      241 tcacccgatc tcgcggtcgt cctggtgggc gccgagccag cgcgagtagc gcgccatcgc
      301 ctccggcatg tgcgactcgc cctcggccgc gcgactgacc ccgaacaccg tgcccgggaa
      361 gtcgagccgc ttctggatgg cccagatctc gtcgtggcgg tcgggcgaga ggatcgctgc
      421 gggatccccg accgcgaccc agttgatcgg cacgaacttg tcgtccggca gccggctgtt
      481 cacgtgcagc acgccgccga tgcggatcgc cgcgcgagcg ccgacgcgcg aaccggggaa
      541 cgcggcggcg ttggtcgcga tgaacgcgtc gtcgccgatc accgcgccgt tgatgtgggc
      601 gtgcggcccg accagcacgt tgtcgccgag cgtggcccgg tagcccgtgc gcccgcggat
      661 cacggcgttc tccatcacca cgcagcgctc gccgatccgc accggcccgc cctcggccac
      721 caccaccgcg ccgaacagga tccggctgcc ggagccgatc tgcacgtcgc cgcacacgac
      781 ggcgttcggc gccacgtaca cgtcgtcggc gatctgcggc ctggcgtcgc ggtgctcgag
      841 gatcacgcga tcaccgcctc gacctcgatc tccgagcgca gcgagttggc gacgtagcag
      901 tagcggtgcg cgagctccac gagccgcagc accttctgct cgctcgcgcc gtcgccgacg
      961 acgatgcgcg ggcgcagcac gatccgctgt atgcgcgcgg gctcatcgga ctcgtccatg
     1021 atcccctccg cgtcgtcgct gtactcgagc acgtcgacgc gggccttcga cgcgatcgcc
     1081 aggaactcga ggagctggca cgcggacgcc gccgccacga tgagctgctc gggattcagc
     1141 agacgcgggt cgccgccgaa ggtggcgtcg cccgagaggc gcagctcctg cccggcggag
     1201 gggacgcgca acgtgtgctc gcggctgtag ctctcgtagc cgccggcggt cgatccgcgc
     1261 caggcgacct gggtcgcgta gcgatgcacg gtcacgggtc gatcagcact ccggggttga
     1321 ggatcgcgcc cgggtcgagc tcggccttcg ccgcccgcag ggcgcgcgcg aacgcctcgg
     1381 gccgctgacg gtcgtaccag gggcggtggt cgcggccgac cgcgtggtgg tgcgtgatcg
     1441 tgccgccgcc ggcgatcacg gcgtcggagg ccgctccctt gacggcgtcc cactgctcga
     1501 gctcggcgcc gcggttcgcc ggcgcgagga tcgtgaagta cggggccggc ccgtccgggt
     1561 acacgtgcgt gaagcggcag gtgaccgtgc cgccgccaca gacctcgcgc agggactcct
     1621 ccgctcgcgc gcgcacgtcc gcgacgaagc cctcgagccg gtcccacgtc acggcggtct
     1681 cgaaggtctc gctgaacacg cccgcgagaa tcatcgtgtc gcgcaggtag ggagccgaga
     1741 cgaacgcctg gcgccaggcg ccctcgccct ccgtgcggcc accgtcggcg ccgggcccgc
     1801 ggtgccgcgc gccgtccgtg gcgctgccgc cgtggtcgcg gcacagctcg agcgcgcggt
     1861 ccatccaggg ctcgagcgga tggtcggccg actcgaaccc gagcacgagc agggccgcgc
     1921 cgtccgacgc ggcgccggtg agcgcggcct cgccgggatc gagcagccgg caattggccg
     1981 ggtcgagcgc cgactgcgac agcgcgcgca ccgcagcggc gcccgcctcg aaggtctcga
     2041 agcgcacgga cgcggacgag cgccagagcg gccgcgggcg cacgcgcatc cacgcctccg
     2101 tcaccacccc gaggatgccc tccgacccga gcatcaggcg gtcggggctc ggcccggcgc
     2161 cggagccggg cagccggcgg ctctcccacg tccccgaggg ggtgatcgcg cgcaccgact
     2221 cgaccaagtc gtcgatgtgc gtgagccgcg tggcgaagtg gccgcccgcg cgcgtgacga
     2281 tccaaccgcc gagcgtcgag tactcgaacg actgcgggaa gtgacgcagc gtgaggccgt
     2341 gttcgcggag ctgcgcctcg agcccgggcc cgagggcgcc ggcctggatg cgggcggcgt
     2401 gggaggcggc gtccacctcc agcacgcggt cgagcgcgcg caggtccacc gtgaccgtgc
     2461 ctgcgtaggc gtcgcccacg cgcggctcca cgccgccgac cacgctggtg ccgccgccga
     2521 acgggatcgc cgcggcgccg gcgtcggcgc accacgacag caccgcctcg acctcgacct
     2581 cgtcgcgcgg gcgcgccacc acgtcgggca cgtgctcgag ctgcccgcgc atcgcgcgca
     2641 cgatgtcgcg gtagccgcgc cccaccgagt ggagggcgcg ctcgtaggtg tcgctggagc
     2701 agatcgccgc cagcgagcca ggcggttcga gccgcggcgc cggcagatcg aggtcactta
     2761 gcgccaccgg gcgctccggc tcggcgggct cgaggccgag gtgggcgcgg atcccggcgg
     2821 ccgcctcgcg cacctgctcg tgcgagggct gctggtcctc gtagccccag ccccagaact
     2881 tcaggcgacg tgtcgcgacg gccatggcgg gtagcagctt cgcagaggaa gcgccgtgcc
     2941 gtaagcgaaa agctattgtg gagtgcactc gatggccacg cggacgacga ccgtccagat
     3001 caccctgccc gagatgggcg agtcggttac cgaaggaacc gtcctagagt ggctcaaggt
     3061 cgagggcgac cgcgtcgaag ccgacgaggg ggtagttgag gtctcgaccg acaaggtcga
     3121 cgcggaggta cccgctcccg ccagcggcat cctcaagcgg atcctggtcc agcccgacga
     3181 gaccgtgccg accggcaccg tgctgggcga gatcgaggtg aacggcgacg gcggccccgc
     3241 cgccgcgagc gacggcaacg gcggggatcc ggagtccgcg gccgcggagc acccgcagtg
     3301 gggcgcagac gagtccgccg ccgagctggt cgagcacggc gccgacgccg aggaggcgca
     3361 ccaggccgcc gcggaggcgg tcgggtcggt gggcgacggc tcggcgcagg cctccgatcc
     3421 cccgcccgac gagccggccg cggacggccc gagcgagctg accgacgtag cgatgcccga
     3481 gatgggcgag tccgtgtccg aggggaccgt gctcgagtgg ctcgtgagcg tcggcgacga
     3541 ggtcgcggtg gacgacgggc tcgtggagat ctcgaccgac aaggtcgacg ccgaggtgcc
     3601 ctcgccggtc gccggcgtga tcagggagat cctcgtgcag cccgacgaga ccgtcacgaa
     3661 cggcaccgtg ctgtgccgca tcgctcccgg cggcgggggc gcgaccccgc ggatgccagc
     3721 cggcgagacc gtgcaccgca ccgacgaccc gcagcccgag gcgcgcccgg cgcagaccgc
     3781 cgacggcggc aacggctcta cccacgccac cccggtcgcc gcgcggatgg cgagcgcgaa
     3841 gggcctcgac gtgagcgggc tcgagggctc cggcccgcgc gggcgcgtga cgaaggccga
     3901 cgtggaggcc gcgctcgaca acggcgggtc ggccgcgctc accggcggca acggcgccgc
     3961 cccgcgcgag gccgcgcgca cccccatccg cggccccgcg gccacgctcg cccgcttcat
     4021 gaacgagagc cgctcgatcc cgacggccac cagcttccgc acgctcgagg tcgacgtgct
     4081 cgccgcccgc cgcaccgccc tcaaggacgc cgggcgcaag ctctccttca cccacctgat
     4141 cgcctgggcc atcgtgcagg cagcgcgcga catgcccgtc atgacgcacg cgtacgagga
     4201 ggtcgacggc aagccgcacc gcgtccagcc cggcggcgtc agcctcgggc tggcggtcga
     4261 cgtcgagcgc aaggacggat cgcgctcgct ggtcgtgccc gtgctgaggg acgccgacgc
     4321 gctcgaccta gcagcgttcg tcgag
//
LOCUS       DASVPL010000164        10934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_257014, whole genome shotgun sequence.
ACCESSION   DASVPL010000164 DASVPL010000000
VERSION     DASVPL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10934
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257014"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..2154
                     /locus_tag="VF517_08430"
     CDS             115..2154
                     /locus_tag="VF517_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8053006.1"
                     /translation="MRRAALAALVTLAALAAAAPASAAGPLPGCADPDVPGGEWRSFG
                     HDLSNTRVQPREKSISPADVPLLTPAWAFSTEQVQAEGDFTGTPIVADGCLYAGTNRG
                     WLFALNADTGKLVWKAKLPRGGSINNTVGIADRACGKTVKYKKKVVKRRGKRKVIRKR
                     VAKTRYCPTVFVGATRTREGEGCPPGEKCIGPYAAAYDQATGKLAWVTSPLDSQNGAD
                     TYGSAMVFDGVLILGVSGGAAELDDEADRGPFQGSLNFLDTANGRVMKKTYTIHPPHQ
                     PDDDFAGATVWSTPAIDTEDKVAYAGTGNPYKPQAEHRNANSVLKYDLDRKSPRFGEI
                     IGAYKGVVDEYLPGLSEAPCFDIPNNHPPYYPQGVGSCGDIDLDFGASPNLFRGPDGR
                     KLVGAGQKSGVYHVFDAKTMKGEWTQIAGPPSQVGGIVGSTAHDGDSVFGPITIGGYV
                     WSLGAEDGHHRWVAPLADGTHWGNPVAVANGVVYTMDAGGSLNAFDARNGTPLAKRPV
                     ALGGSKSAASLSWGGVSIARNTVYAATGLGSLPQGYIVAFRPGAPQDLPEDAAETATG
                     GGGGGGGGGGGGEGGSAAGGAIVAVPGSTYSTYATPVMTTRPGGPLSFMNFDLPQHDV
                     VSDDKGPDGRPLFQSKLSGLGEIAPIEGLDRVKSGSTYGFFCSLHPGMRGSLIVQ"
     gene            2151..3071
                     /locus_tag="VF517_08435"
     CDS             2151..3071
                     /locus_tag="VF517_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8053007.1"
                     /translation="MTPGRIAALLAVLALLVVGAVAVADTITGTANNDELNGTAGEDT
                     ITGGSGDDRINGGGGNDVLDGSGDGDTIDGGDGNDTIYGGNCRRGQLGRICDNSGAER
                     LSGGPGNDELRVNQCILVDCNDDPLIGVDSVLDGGPGDDRLSGAAGNDSLTGGDGNDK
                     LRGIMGDDRHDGGAGNDLLRGGEGADLMLAGAGVDRLWGQQDADVMNGGADADRVYGG
                     DGSDSLNGAEGNDRLWGQAGADGLEGAAGNDRLSGGAGKDRMFGGLGADRLLARDGAR
                     DVVRCGDGRDVAYVDRRDSVSGCERVFVAR"
     gene            3147..3623
                     /locus_tag="VF517_08440"
     CDS             3147..3623
                     /locus_tag="VF517_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8053008.1"
                     /translation="MTRSPAAEQHLRDLAHLRRARDLMDREYARPLEVAALARAALMS
                     TAHFSRQFRAAYGETPYAYLMTRRIERAKALLRRGDMSVTEVCLEVGCSSLGSFSARF
                     TEIVGETPTAYRARDHGALASVPGCIARDLTRPGRRPPTEQDRRSARGAGSIALPA"
     gene            3620..4027
                     /locus_tag="VF517_08445"
     CDS             3620..4027
                     /locus_tag="VF517_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8053009.1"
                     /translation="MNLTLSQCFVLVHDPELALAFYRDQLGLEMRNDVARGDFRWITV
                     GSASQPGVAIVVTNYLNGSPADVEALEALLAKGALNGVHFHTDDLDSTFEKLRDSGAE
                     IVQEPTEQPWGTRDCAVRDPSGNMIRIDQPPAA"
     gene            4076..6376
                     /locus_tag="VF517_08450"
     CDS             4076..6376
                     /locus_tag="VF517_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX8053010.1"
                     /translation="MNNLRDVSIEIPKRRLTVFTGVSGSGKSSLVFGTIAAESQRLIN
                     ETYSAFVQGFMPTLARPEVDVLEGLTTAIIVDQQRMGSDARSTVGTATDALAMLRILF
                     SRLGQPHVGPPGAFAFNVPSVRASGAITVERGSKKTVRATFSRIGGMCPRCEGMGTIS
                     DFDLSQLYDDSKSLNEGALTIPGYSMDGWYGRIFRGCGFFDPDKPIKKFKKSELNDLL
                     HKEPTKIEVDGVNLTYEGLIPRIQKSFLSKDADALQPHVRAFVERAVTLSACPECDGT
                     RLSEAARSSRIDGINIAEACAMQISDLAGWVRGLDGPTVAPLLATLRHTLDSFAEIGL
                     GYLSLDRSSGTLSGGEAQRVKMIRQLGSSLTDVTYVFDEPTTGLHPHDIQRMNELLLR
                     LRDKGNTVLVVEHEPETIAIADHVVDLGPGAGSAGGTVCFEGTVEDLRGGDTLTARHL
                     DDRAALKETVRTPTGTLEIRGATAHNLQDVDVDIPLGVLVVVTGVAGSGKSTLIHGSL
                     AGRDGVVSVDQTPIRGSRRSNPATYTGLLDPIRKAFAKANGVKPALFSANSEGACPNC
                     NGAGVVYTDLAMMAGVATTCEECGGKRFQAAVLDHHLGGLDISEVLAMSVTEAGEFFG
                     SGEASTPAAQRVLERLADVGLGYLTIGQPLTTLSGGERQRLKLATRMAEKGGVYVLDE
                     PTAGLHLADVEQLLGLLDRLVDSGKTAIVIEHHQAVMAHADWIVDLGPGAGHEGGRVV
                     FEGTPADLAAARSTLTGEHLAAYAAA"
     gene            complement(6386..7048)
                     /locus_tag="VF517_08455"
     CDS             complement(6386..7048)
                     /locus_tag="VF517_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8053011.1"
                     /translation="MAARITIVSARFEDLVSIGLGSLISEDPNLELIASDIDLDQLEA
                     VLEEQKPSVVLLNFGSLPNPAMIYQLHQAHPDTRIVVLANKPTAAECNQMLSFGATAC
                     ISKETQGRDIINAIHLASRGMHVLPRSANATAGRDERFDLPAPDLLTPREADVLELLQ
                     RGLTNAQIAHELSIGVETVRTHARNIYSKLGISTRRDLARLGNEDRGAPRRHLEPVGP
                     VT"
     gene            7484..9175
                     /locus_tag="VF517_08460"
     CDS             7484..9175
                     /locus_tag="VF517_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8053012.1"
                     /translation="MSPGRGLALIGSVLTMDPAAPEAGGVGIADGRIEVVGSADEVRA
                     AMGEAAELVELGDELILPAFIDAHHHFCMAAFDRRTPDLHLEPGSTIADVLALVEQAA
                     AAPGDGWLRLQGYDPSKLRERRAPTAAELDEACSDRPLLLIAYSFHEACLNSRGLAEM
                     GWDASSADPPHGVLVRKRGRLTGELIEGAMFLAEARSRDSLLETAADAWIAECEAHGR
                     DLLAAGIVRVGDAAVPPTFERLYERAAEAGRLPVIVHRMPVAAASIIEPRTEAPPTGS
                     GPARTPIGPAKLFMDGAERCAICLSMTETAHAAALTLRTAVGGEGLAALRAATSVEWT
                     RGADGLMHAGVMFWDQDRLDAAVGAAARAGLQVAQHAIGNEAIALALTALEHEGAPLA
                     DLPGVPRLEHVTFLDPALARRIGDVGAMAVVQPYFVYDMVGDFTALTPLPQPNIVLPL
                     RALLDAGVELAGSSDHPVSGYDVLAAVEAAVRRHTRLGRVSGPDEAITAEQALRAYTL
                     GSARALGVEDEAGSLEAGKRADVVILSGDPRRTATPTELSVRRTYLGGELAYQAA"
     gene            complement(9172..10074)
                     /locus_tag="VF517_08465"
     CDS             complement(9172..10074)
                     /locus_tag="VF517_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEX8053013.1"
                     /translation="MKLAYWPGCVSRGFTPELHGAMSAVAPKLDLELIELDRANCCGA
                     GVIAEHNQELVDTLNARTFGLAQQVDGAVGMMNICSTCQGAQSECQERLDADTAYRAH
                     INEHLEPEDIAYERDGAWWNKNFLWLLVEEIGLDTLRERVVRPLTGLRVGPFYGCYIV
                     RPTRRLGFDEHPDRDKYLDWVIEAVGAESVPYAGSHKCCGFPIVTMNRTTSLRQAGRH
                     MADAIDAGADCVVTPCPLCHLNLDLQQPEAAPFVERDLGMPVLHLPQLVGLALGLEPK
                     ELGMNRHVVKTDDVQAKVAELAAV"
     gene            complement(10071..>10934)
                     /locus_tag="VF517_08470"
     CDS             complement(10071..>10934)
                     /locus_tag="VF517_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX8053014.1"
                     /translation="KLSTAAESAPNGSIIVEPMGNMPVLKDLIVDMDAVHWKKVQRVV
                     PWLLPEGDPPEREYIVEPESMMDITQSMACIHCGACVSACLSMEVDPDFIGPAALAKA
                     YRFVGDPRDAHTEDRLLDLAEDPHGIYDCTHCFACIEVCPKDVAPMNQIMRLRRRATS
                     MDIKDRNNGYGHEKAFVDMIGKYGTLHESQLIPRSYGQGSLIRSQVDPAALKELAAKL
                     PTVLRGVKSGKVKIKTALIHHKLADQDQIKRIYQEIEDKDERLELNLYIVGEGGDEQE
                     ADQMAESGQPA"
BASE COUNT         1578 a         3941 c         3938 g         1477 t
ORIGIN      
        1 cctccagggc ggcggcacgg agcgggagtc ggcgccgctg gcttcgcacg tcgcgccggg
       61 cgctcggacc aaggaagagg tcgtgaagag cgtgtacggc ggcgagtggt cgccgtgagg
      121 cgcgcggcgc tcgcggcgct cgtcaccctc gccgcactgg cggccgcggc ccccgcgtcg
      181 gcggccgggc cgctccccgg gtgcgcggat cccgacgtgc cgggcggcga gtggcgcagc
      241 ttcgggcacg acctctcgaa cacccgcgtg cagccgcgcg agaagtcgat ctcgcccgcc
      301 gacgtgccgc tgctcacgcc cgcgtgggcg ttctcgaccg agcaggtgca ggccgagggc
      361 gacttcaccg gcaccccgat cgtcgccgac ggatgcctct acgccggcac caaccgcggc
      421 tggctgttcg cgctgaacgc cgacaccggg aagctggtgt ggaaggcgaa gctgccgcgc
      481 ggcgggtcga tcaacaacac cgtcggcatc gccgaccgcg cctgcggcaa gaccgtcaag
      541 tacaagaaga aggtcgtgaa gcggcgcggc aagcgcaagg tgatccgcaa gcgcgtcgcg
      601 aagacgcggt actgcccgac ggtgttcgtc ggggccaccc gcacgcgcga gggcgagggc
      661 tgcccgcccg gcgagaagtg catcggaccg tacgcggccg cgtacgacca ggcgaccggc
      721 aagctcgcct gggtcacgtc gccgctcgac agccagaacg gcgccgacac ctacgggagc
      781 gcgatggtct tcgacggcgt cctgatcctc ggcgtctcgg gcggggcggc cgagctcgac
      841 gacgaggccg atcgcggacc gttccagggc tctctgaact tcctcgacac ggcgaacggg
      901 cgcgtgatga agaagaccta cacgatccac ccgccccacc agcccgacga cgacttcgcg
      961 ggcgcgacgg tgtggtccac acccgcgatc gacaccgagg acaaggtcgc gtacgccggg
     1021 accggcaacc cgtacaagcc gcaggccgag cacaggaacg cgaactcggt gctcaagtac
     1081 gacctcgacc gcaagagccc gcggttcggc gagatcatcg gcgcctacaa gggcgtggtc
     1141 gacgagtacc tccccggcct ctccgaggcg ccgtgcttcg acatccccaa caaccacccg
     1201 ccgtactacc cgcagggcgt cggctcgtgc ggcgacatcg acctcgactt cggtgcctcg
     1261 cccaacctct tccgcgggcc ggatgggcgc aagctcgtcg gcgcgggcca gaagtccggc
     1321 gtctaccacg tgttcgacgc gaagacgatg aagggggagt ggacccagat cgccggcccg
     1381 ccgtcgcagg tcggcggcat cgtcggctcg accgcgcacg acggcgactc ggtcttcggg
     1441 ccgatcacga tcggcggcta cgtctggtcg ctcggcgccg aggacgggca tcaccgctgg
     1501 gtcgcaccgc tcgccgacgg cacccactgg ggcaacccgg tcgcggtcgc caacggggtc
     1561 gtctacacga tggacgcggg cggctcgctc aacgccttcg acgcccgcaa cggcacgccg
     1621 ctcgccaagc gcccggtggc tcttggcggg tcgaagagcg ccgcgtcgct gtcgtggggc
     1681 ggcgtcagca tcgcccgcaa cacggtctac gcggccacgg gtctgggctc gctcccgcag
     1741 ggctacatcg tggccttccg gccgggcgcg ccgcaggacc tgcccgagga cgcggccgag
     1801 acggccaccg gcggcggtgg cggcggtggc ggtggaggcg gcggtggcga gggcggcagc
     1861 gccgcggggg gcgcgatcgt cgcggtgccg ggatcgacct actcgaccta cgccacgccc
     1921 gtgatgacca cgcgcccggg cggcccgctg agcttcatga acttcgacct cccgcagcac
     1981 gacgtcgtct ccgacgacaa gggccccgac ggccggccgc tgttccagag caagctgagc
     2041 gggctaggcg agatcgcgcc gatcgagggg ctcgaccgcg tgaagtccgg ctccacgtac
     2101 gggttcttct gcagcctcca cccgggcatg cggggcagcc tgatcgttca gtgacgcccg
     2161 gccgcatcgc agcgctgctc gccgtgctcg cgctgctggt cgtgggcgcg gtggccgtgg
     2221 ccgacacgat caccggcacg gcgaacaacg acgagctcaa cggcacggcc ggcgaggaca
     2281 ccatcaccgg cggcagcggc gacgaccgca tcaacggcgg cggtggcaac gacgtgctcg
     2341 acggcagcgg cgacggcgac acgatcgacg gcggcgacgg aaacgacacg atctacggcg
     2401 ggaactgccg ccgcggccag ctcggccgca tctgcgacaa ctcgggcgcc gagcggctct
     2461 cgggcggccc gggcaacgac gagctgcgcg tcaaccagtg catcctggtc gactgcaacg
     2521 acgatccgct gatcggggtc gacagcgtgc tcgacggcgg gcccggcgac gaccgcctga
     2581 gcggcgccgc cggcaacgac agcctcaccg gcggcgacgg aaacgacaag ctgcgcggca
     2641 tcatgggcga cgatcgccac gacggcggcg ccggcaacga cctgctccgc gggggagagg
     2701 gcgccgacct gatgctggcc ggggccgggg tcgatcggct ctgggggcag caggacgccg
     2761 acgtgatgaa cggcggggcc gatgcggatc gcgtctacgg cggcgacggc agcgactccc
     2821 tgaacggcgc cgagggcaac gacaggctct ggggccaggc gggcgcggac gggctggagg
     2881 gcgccgcggg caacgaccgc ctgagcggcg gcgccggcaa ggaccgcatg ttcggcgggc
     2941 tgggcgccga ccgcctgctc gcgcgcgacg gcgcgcgcga cgtggtccgc tgcggcgacg
     3001 gccgcgacgt ggcgtacgtg gaccggcgcg acagcgtgtc gggctgcgag cgcgtcttcg
     3061 tcgcgcgcta agcaggcccg ggcacgcctc ggtgcgcggc gccccccggc tagcgggcgc
     3121 ctccggcccc gtggcacaat tccgcggtga cccgcagccc cgccgcggag cagcacctgc
     3181 gcgacctcgc gcacctgcgc cgcgcccgcg acctgatgga tcgtgagtac gctcgcccgc
     3241 tcgaggtcgc cgcgctcgcc cgcgccgcgc tgatgtccac cgcacacttc tcgcggcagt
     3301 tccgcgcggc gtacggggag acgccctacg cctacctgat gacgcggcgc atcgagcggg
     3361 cgaaggcgct gctgcgccgc ggcgacatgt cggtcaccga ggtctgcctg gaggtcggct
     3421 gctcgtcgct cggctcgttc agtgctcgct tcaccgagat cgtgggcgag acgccgacgg
     3481 cctaccgggc ccgtgaccac ggcgccctcg cgagcgtgcc gggctgcatc gccagggatc
     3541 tcacccgccc gggccggagg ccgccaaccg agcaggatcg gagaagcgcg cgcggcgccg
     3601 gctccatagc cttgccggca tgaacctcac cctctcccaa tgcttcgtcc tcgtccatga
     3661 ccccgagctc gcgctggcct tctaccgcga tcagctcggc ctcgagatgc gcaacgacgt
     3721 ggcccgcgga gacttccgct ggatcaccgt ggggtccgcc tcccagccgg gcgtcgcgat
     3781 cgtcgtcacg aactacctga acggcagccc cgccgacgtg gaggcgctcg aggccctgct
     3841 cgccaagggc gccctgaacg gcgtgcactt ccacaccgac gacctcgact ccaccttcga
     3901 gaagctccgc gactcgggcg ccgagatcgt gcaggagccg accgagcagc cgtggggcac
     3961 gcgcgactgc gccgtgcgcg atccgtccgg caacatgatc cgcatcgacc agccgcccgc
     4021 cgcgtagttg cacacggccg atagccacga cctcatccgc gttcacggtg cgcgggtgaa
     4081 caacctgagg gacgtcagca tcgagatccc gaagcgccgg ctgaccgtgt tcaccggcgt
     4141 ctccggctcg ggcaagagct cgctggtgtt cggcacgatc gcggccgagt cgcagcggct
     4201 gatcaacgag acctacagcg cgttcgtgca gggcttcatg ccgacgctgg cgcgcccgga
     4261 ggtcgacgtg ctcgaggggc tcacgaccgc gatcatcgtc gaccagcagc gcatgggctc
     4321 cgacgcccgc tccacggtcg gcaccgccac cgacgccctc gcgatgctgc gcatcctctt
     4381 cagccgcctc gggcagccgc acgtcgggcc gcccggcgcg ttcgccttca acgtcccgtc
     4441 ggtacgggcg agcggcgcga tcacggtcga gcgcggctcg aagaagaccg tgagggcgac
     4501 cttcagccgc atcggcggca tgtgcccgcg ctgcgagggc atgggcacga tctccgactt
     4561 cgacctgtcc cagctctacg acgactccaa gtcgctcaac gagggcgcgc tcacgatccc
     4621 cggctacagc atggacggct ggtacggccg catcttcagg ggctgcggct tcttcgaccc
     4681 cgacaagccg atcaagaagt tcaagaagag cgagctgaac gacctgctcc acaaggagcc
     4741 gaccaagatc gaggtcgacg gcgtcaacct gacgtacgag ggcctgatcc cgaggatcca
     4801 gaagtcgttc ctgtccaagg acgccgacgc gctccagccg cacgtccggg cgttcgtgga
     4861 gcgggccgtg acgctgagcg cctgccccga gtgcgacggg acccggctca gcgaggcggc
     4921 ccgctcgtcg aggatcgacg ggatcaacat cgccgaggcc tgcgccatgc agatcagcga
     4981 cctcgccggc tgggtgcggg gtctggacgg accgaccgtc gcgccgctgc tggcgacgct
     5041 gcggcacacc ctcgactcgt tcgccgagat cgggctcggc tacctcagcc tcgaccgctc
     5101 gtccggcacg ctgtcgggcg gcgaggcgca gcgcgtgaag atgatccgcc agctcggctc
     5161 gtcgctcacc gacgtcacct acgtgttcga cgagcccacc acgggcctgc acccgcacga
     5221 catccagcgg atgaacgagc tgctgctgcg gctgcgcgac aagggcaata cggtgctcgt
     5281 cgtggagcac gagccggaga cgatcgcgat cgccgaccac gtggtcgacc tcggccccgg
     5341 cgccggctcg gcgggcggca ccgtctgctt cgaggggacc gtcgaggacc tgcgcggcgg
     5401 cgacaccctc accgctcgcc atctcgacga ccgcgccgcc ctcaaggaga cggtgcggac
     5461 gcccaccggc acgctcgaga tccgcggcgc gacggcgcac aacctccagg acgtcgacgt
     5521 ggacatcccg ctgggcgtcc tggtcgtggt caccggcgtg gccgggtcgg gcaagagcac
     5581 cctgatccac ggctcgctgg cgggccggga cggagtcgtg tcggtcgacc agaccccgat
     5641 ccgcgggtcg cggcggagca acccggcgac gtacacggga ctgctcgacc cgatccgcaa
     5701 ggcgttcgcg aaggccaacg gcgtgaagcc ggcgctgttc agcgcgaact ccgagggcgc
     5761 ctgccccaac tgcaacggcg ccggcgtcgt gtacaccgac ctggcgatga tggccggcgt
     5821 cgccaccacg tgcgaggagt gcggggggaa gcggttccag gcggccgtgc tcgaccacca
     5881 cctcggtggc ctcgacatca gcgaggtgct cgcgatgtcg gttaccgagg ccggggagtt
     5941 cttcggctcc ggcgaggcga gcacgccggc cgcgcagcgc gtcctcgaac ggctcgccga
     6001 cgtgggcctg ggctacctca ccatcggcca gccgctcacc acgctgtccg gcggtgagcg
     6061 ccagcggctc aagctggcca cccgcatggc ggagaagggc ggcgtgtacg tgctcgacga
     6121 gccgaccgcg gggctccacc tggccgacgt cgagcagctg ctcggcctgc tggaccggct
     6181 cgtcgattcc ggcaagacgg ccatcgtcat cgagcaccac caggcggtga tggcgcacgc
     6241 cgactggatc gtcgacctcg ggcccggcgc cggccacgag ggcggccggg tcgtcttcga
     6301 gggcaccccc gccgacctcg ccgccgctcg ctccaccctc accggggagc acctcgcggc
     6361 ctacgcggcc gcctgacccg cgcgcctagg tgacggggcc gaccggctcc aggtgccggc
     6421 gcggcgcgcc gcgatcctcg ttgccgaggc gggccaggtc gcggcgagtc gagatgccga
     6481 gcttcgagta gatgttgcgc gcgtgcgtgc gcacggtctc cactccgatc gagagctcgt
     6541 gcgcgatctg cgcgttggtg agcccgcgct ggagcagctc gagcacgtcg gcctcgcggg
     6601 gggtgaggag gtcgggcgcg gggaggtcga agcgctcgtc gcggccggcg gtggcgttgg
     6661 ccgaccgcgg caggacgtgc atcccgcgcg acgcgaggtg gatcgcgttg atgatgtcgc
     6721 ggccctgcgt ctccttggag atgcacgcgg tggcgccgaa cgagagcatc tggttgcact
     6781 cggcggcggt cggcttgttc gccagcacga cgatgcgcgt gtcggggtgg gcctggtgta
     6841 gctggtagat catcgccggg ttcggcagcg acccgaagtt gagcagcacc accgacggct
     6901 tctgctcctc gagcacggcc tcgagctggt cgaggtcgat gtcgctggcg atcagctcga
     6961 ggttcgggtc ctcggagatg agcgacccga ggccgatcga gacgaggtcc tcgaaccggg
     7021 ccgagacgat ggttatgcgg gcggccacgg cgtggagctt accggatccg ggggatgccg
     7081 ggggagatgc ccctacccat cccctcaccc tcagctcccc cggtgggcgc cctggccctc
     7141 ccgcaaagat cacccccagt tcacacgtgg cgggggacgt gggaatgcct gatgtaggga
     7201 accattcaac cagtcggctg ggaggcccga caagagactt tcatagcttc ccagggcgcg
     7261 taggggtacg ccgcaccgcg aagacccgtc acgagagtgg cgggtttttc gcgttgaggg
     7321 gcaaaagtgg cggagccggc tccgggcgcg agcctgcgga tcccgcgcga gcgggggtcc
     7381 cgggggggca gcggagccac gcgagccggg gccgggggcc tcggagcccg cgcgacccgc
     7441 ggcccgggag ccgcccacgg ccccgcgccg gtaatgtcgc gccatgtccc cgggcagggg
     7501 cctcgccctc atcggaagcg ttctgacgat ggatccggcg gcaccggagg ccggcggcgt
     7561 ggggatcgcg gacgggcgca tcgaggtcgt cggctccgcc gacgaggtgc gcgcggcgat
     7621 gggcgaggcg gccgagctgg tggagctcgg cgacgagctg atcctgccgg cgttcatcga
     7681 cgcccaccac cacttctgca tggccgcctt cgaccggcgc acgccggacc tgcacctgga
     7741 gcccgggagc acgatcgcgg acgtgctcgc tctcgtggag caggccgccg ccgcgcccgg
     7801 cgacggctgg ctccgcctcc agggctacga cccgagcaag ctgcgcgagc gccgcgcgcc
     7861 gacggcggcc gagctcgacg aggcctgctc cgatcgcccg ctgctgctga tcgcctacag
     7921 cttccacgag gcctgcctca actcccgcgg cctcgccgag atgggttggg acgcgagctc
     7981 ggcggatccg ccgcacggcg tgctggtgcg caagcgaggc cgcctgaccg gcgagctgat
     8041 cgagggcgcg atgttcctgg ccgaggcccg ctcgcgcgac tcgctgctcg agaccgccgc
     8101 cgatgcgtgg atcgccgagt gcgaggcgca cggccgcgac ctgctcgccg ccgggatcgt
     8161 gcgcgtgggc gacgccgccg tgccgccgac gtttgagcgc ctctacgagc gcgccgcgga
     8221 ggccgggagg cttccggtga tcgtgcaccg catgccagtc gccgcggcga gcatcatcga
     8281 gccgcgcacc gaggcgcccc cgaccggcag cggacccgcg cggaccccga tcggcccggc
     8341 gaagctgttc atggacggtg ccgagcgctg cgccatctgc ctgtcgatga ccgagaccgc
     8401 gcacgcagcg gcgctgacgc tgcgcacggc ggtgggcggc gagggactgg ccgcgctccg
     8461 cgccgcgacc tccgtcgagt ggacgcgcgg cgcggacggt ctcatgcacg ccggcgtgat
     8521 gttctgggac caggaccggc tcgacgccgc cgtgggcgcg gccgcgcgcg ccggcctcca
     8581 ggtggcccag cacgcgatcg gcaacgaggc gatcgccctc gcgctcaccg ccctcgagca
     8641 cgagggcgcc ccgctcgccg acctgcccgg ggtgccgcgc ctcgagcacg tgaccttcct
     8701 cgaccccgcc ctcgcgcgcc ggatcggcga cgtgggcgcg atggccgtgg tccagccgta
     8761 cttcgtctac gacatggtgg gcgacttcac ggcgctcacg ccgctgcccc agccgaacat
     8821 cgtcctgccg ctgcgcgcgc tcctcgacgc cggggttgag ctggccggca gctccgacca
     8881 ccccgtctcc ggctacgacg tgctcgccgc cgtggaggcc gccgtccggc gccacacccg
     8941 gctcggccgg gtcagcggcc ccgacgaggc gatcacggcc gagcaggcgc tgcgcgcgta
     9001 cacgctcggc tcggcgcggg cgctcggcgt cgaggacgag gcgggcagcc tcgaggccgg
     9061 caagcgcgca gacgtcgtga tcctctccgg cgatccccgc cgcactgcca cgccgaccga
     9121 gctgagcgtg cgccgcacct acctcggcgg cgagctggcc taccaggccg cttagaccgc
     9181 ggcgagctcc gcgaccttcg cctgcacgtc gtcggtcttg acaacgtgcc ggttcatgcc
     9241 cagctccttg ggctccagcc cgagcgcgag gcccacgagc tgcgggaggt gcaggaccgg
     9301 catgccgagg tcgcgctcca cgaacggggc ggcctccggc tgctggaggt ccaggttcag
     9361 gtggcagagc gggcagggcg tgacgacgca gtcggccccc gcgtcgatcg cgtcggccat
     9421 gtggcggccg gcctggcgca gcgaagtggt ccggttcatc gtgacgatcg ggaagccgca
     9481 gcacttgtgg ctgccggcgt agggcaccga ctcggcgccg accgcctcga tcacccagtc
     9541 caggtacttg tcgcggtccg gatgctcgtc gaagccgagc cgccgcgtgg gccggacgat
     9601 gtagcacccg tagaacgggc cgacgcgcag gccggtaagc ggccgcacca cgcgctcgcg
     9661 cagggtgtcg agcccgatct cctcgaccag cagccacagg aagttcttgt tccaccacgc
     9721 gccgtcgcgc tcataggcga tgtcctcggg ctcgaggtgc tcgttgatgt gcgcgcggta
     9781 cgccgtgtcg gcgtcgaggc gctcctggca ctccgactgc gcgccctggc aggtcgagca
     9841 gatgttcatc atcccgacgg cgccatcgac ctgctgggcc agcccgaacg tgcgggcgtt
     9901 cagcgtgtcg acgagctcct ggttgtgctc ggcgatcacg cccgcaccgc agcagttcgc
     9961 gcggtcgagc tcgatcagct ctaggtcgag cttcggcgcc acggccgaca tcgcgccgtg
    10021 cagctcgggg gtgaagccgc gcgagacgca gccgggccag tacgcgagct tcatgcgggt
    10081 tgtcctgact cggccatctg gtctgcctcc tgctcgtcgc cgccctcgcc cacgatgtag
    10141 aggttgagct cgaggcgctc gtccttgtcc tcgatctcct ggtagatccg cttgatctgg
    10201 tcctggtcgg cgagcttgtg gtggatcagg gcggtcttga tcttgacctt gcccgacttg
    10261 accccacgca ggaccgtcgg cagcttggcc gcgagctcct tgagcgccgc ggggtcgacc
    10321 tgcgagcgga tcagcgagcc ctggccgtag gagcgcggga tgagctgtga ctcgtgcagc
    10381 gtgccgtact tcccgatcat gtccacgaac gccttctcgt ggccgtagcc gttgttgcgg
    10441 tccttgatgt ccatcgaggt cgcgcgccgg cgcaggcgca tgatctggtt catcggcgcc
    10501 acgtccttgg ggcagacctc gatgcaggcg aagcagtggg tgcagtcgta gatcccgtgc
    10561 gggtcctcgg cgaggtcgag cagccggtcc tcggtgtgag cgtcgcgcgg gtcgcccacg
    10621 aagcggtacg ccttcgccag cgcggcgggg ccgatgaagt cggggtcgac ctccatcgac
    10681 aggcacgccg acacgcacgc tccgcagtgg atgcaggcca tcgactgcgt gatgtccatc
    10741 atcgactcgg gctcgacgat gtactcgcgc tcgggcgggt cgccctcggg caggagccac
    10801 gggaccacgc gctgcacctt cttccagtgc acggcgtcca tgtccacgat caggtccttg
    10861 agcacgggca tgttgcccat cggctcgacg atgatcgacc cgttcggcgc ggactccgcc
    10921 gccgtcgaga gctt
//
LOCUS       DASVPL010000165        21718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_215696, whole genome shotgun sequence.
ACCESSION   DASVPL010000165 DASVPL010000000
VERSION     DASVPL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 21718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21718
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215696"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VF517_08475"
     CDS             <1..685
                     /locus_tag="VF517_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX8053015.1"
                     /translation="GPILIDTGLHPSVAVDKKQNLGRFFASTVARSWEMDPDQALPDQ
                     LRERGIEPSDVRLVVMTHLHLDHASGMVQFPEATFVISRQEWERASEPRAVTSGYVTS
                     HFDHAFDYRLLDFDDPGVGSFAGFGRSFDLLGDGSIHVVFTPGHTLGHMSVVLRGDER
                     EVLVAGDAIYTMHALKTGHKPYLMQDSHLFGRSVREIQRYVEQTPSAIVVPGHEMEPF
                     EEARRALAR"
     gene            complement(682..1464)
                     /locus_tag="VF517_08480"
     CDS             complement(682..1464)
                     /locus_tag="VF517_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8053016.1"
                     /translation="MKPQVYKDPRPAEYFTRFHERTRTRKPDFMYEVVRLILTPVLLF
                     FFRTRCIDSQKIPPEGSVIIAPNHFSFMDHFLISVYVRRKVHFVAKSQLFKRPLQFIY
                     THGGVFPVRRGHRDEEMFKTAHSLLSRGKVIVMYAEGGRSRTGELGEAKPGIGRLALE
                     TGAVVVPSAIAGSEGARNWKRLRFPKVTVQYGDPIVFERTVEEPTRDEAQAAANEIFA
                     RTQKVFYGLRRGGRRNAIQTARAARRAAGKAAGNAARRTAAG"
     gene            complement(1525..2994)
                     /gene="glpK"
                     /locus_tag="VF517_08485"
     CDS             complement(1525..2994)
                     /gene="glpK"
                     /locus_tag="VF517_08485"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX8053017.1"
                     /translation="MILAIDQGTTGTTCIVFGADGQPAGRAYSEFEQHFPRPGWVEHD
                     AEEIWDTTLGVARDALADAGTDAGQLAGIGITNQRETVVAWDRDSGEPLHRALVWQDR
                     RTAARCDELRDAGHEPTFRERTGLVLDPYFSGTKIEWLVREGGVDPARAAFGTIDSWL
                     LHKLTGRHVTDYTNASRTLLFDIRSRKWDPELCELLSVDPASLPEVLPSAHVYGETDA
                     LGGSVPVAGIAGDQQAALYGQACSEPGLGKNTYGTGSFVLQNAGTEAPPVQEGVLNTI
                     AWGVGDRVDYAVEASVFVTGAAVQWLRDGLGIIEAAAETEELARSLDSNDGVYFVPAL
                     TGLGSPHWDPYARGTIVGLTRGNGRAHLARAALEAIAYQTVDAVRAIEATSSAPLAEL
                     RADGGAAVNAWLMQFQADVLGVPVVVPEISETTALGAAYLAGVATGFWDEAGTRQMWR
                     EAARYEPRMTADERESLLAEWRRAVERSRAWVSPEADAG"
     gene            complement(2991..3761)
                     /locus_tag="VF517_08490"
     CDS             complement(2991..3761)
                     /locus_tag="VF517_08490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8053018.1"
                     /translation="MRPEIAIVVTAYNEADRLGETLSALGEAFPGARVLVADDASDDG
                     TADVARAAGAEAVTAPRRLGKGGVATLAAERARGGGSAPGGGSAPGGGAAPGGGSAPG
                     GGSAPLLVLCDGDLGGSARHLPALVAAVERGEGDLAVAVFATRVGGGFGLAVGFAHRA
                     IRSLTGLDLRAPISGQRALAPALLDAVLPFAPRFGMEIGMTVDAHRAGFRVVEVELPL
                     EHRATGRTAAGFAHRGRQLRDFVRVYALRRLNRGRDRG"
     gene            3810..4022
                     /locus_tag="VF517_08495"
     CDS             3810..4022
                     /locus_tag="VF517_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEX8053019.1"
                     /translation="MPDVEAHITGTVWKVECKVGDTVEEGDTLVILESMKMEMPVEAE
                     DDGTVSEIRCEEGQSVSEGDTLVVLE"
     gene            4030..4848
                     /locus_tag="VF517_08500"
     CDS             4030..4848
                     /locus_tag="VF517_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8053020.1"
                     /translation="MELASGKLVIDEPASNVVRIRIANPDKRGALDHEILDALADTVR
                     NLDCLCLVITGTAPVFSAGYDIGNFPESTFDGSGGDRGFADAAEKLVAHPFTEAIEAI
                     EAYPYPVVAAINGHAIGGGLELAVTCDVRIAARGSRLGMPPAKIGLIYSYTGLRRFID
                     ICGVGNTSELFYTGRNVEADHAYEMGLVNRVVEADELEEAALAVAREIGTNSPLALRG
                     NKEVMRTLRAHSGDLPAIEARRLVTLRESCFTSGDFIEGIRAFGEKRAPRWSGR"
     gene            4895..6031
                     /locus_tag="VF517_08505"
     CDS             4895..6031
                     /locus_tag="VF517_08505"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8053021.1"
                     /translation="MRAAGFWSYTREDDVGDNGRVARLADQLKAEYGLITGDELELFV
                     DRTSLDWGDQWRTRIDEALATTTFFIPVITPRYFRRPECRKELLTFAGHAQSLGVEEL
                     LLPLLYVEVPDLDEDSEDEAIALVAGTQYEDWRNLRLVDENSADYRQGVNRLATRLAN
                     ISEEVADRPDPLPPQGRSTADGDADEGPGFLELLAEGEKALPRWERTITAFPDVLEEV
                     ATVTEGATDQMSESDARGGGFAARIAITRRLARELDAPAKRIAELGRSYASDLVAIDP
                     AILTLLRWAEEDPDEAATPEAQEFFTSIQALAQASKEATKELKAFVTALDDASQMTRE
                     IRPQARTMREGLRDILDGQAVIDEWERRVSRVTAAVEQQHTAHE"
     gene            6118..7212
                     /locus_tag="VF517_08510"
     CDS             6118..7212
                     /locus_tag="VF517_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8053022.1"
                     /translation="MLFAAVVIFAVVLALIVVFAVLPNLVVSDSAVKAEVDRVKLEND
                     VRRTGAQILAGLLVLGGLFLTARSIRVTREGQLTERFSRAIDQLGHEELDVRLGGIYS
                     LERIARDSASDRGPVNEVLSAWLRRKSRVRRRGTGRRLPEDVRAVLVVLGRRPKAPSS
                     NADRVDLTSIDLRSADLLVLDLPRAVLSDARLDGADLTRAKLNQAHLTRAHFENADLT
                     GAELKKATLTNARFDAADLSETTLTKATCTSGSFIDATLASSVLSRAEFAHADLSRSD
                     LQGAKARKTNFARARLAGANLTRADVRGASFLGASLRGANLSGTTLKRADLRGADLRN
                     AAGLTAEQVAVARCDMTTRFSAPDPATRQD"
     gene            7351..8544
                     /gene="bioB"
                     /locus_tag="VF517_08515"
     CDS             7351..8544
                     /gene="bioB"
                     /locus_tag="VF517_08515"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX8053023.1"
                     /translation="MPPLISYDEALALGEIEDHAEIEALVDRAFAARLEHFGDSTDMC
                     SLVNAKSGGCAEDCGFCAQSKSAESDTPMHAMMTPEQILEHARAAEAAGAHRFCMVTQ
                     GQGLSKKDFQNVLDGAKLVAEHTNLKRCVSVGHISRDRAKALKEVGIQRVHHNVETAE
                     SYYPEVSSTVRYEGRLRTIDAVADAGLETCVGGILNLGESRAQRVEMAFQLAKVDPTS
                     VPINLLNPRPGTKFGDRDFMDPWEAVKWIAIFRLILPKALFRLCGGRVENLGELEPMA
                     VKAGLNGVMMGNFLTTLGSEPEDDRAMFESLGLNTARQPDNGANPRPDNRSGWLEGDS
                     PSTPVDRLIDRQDEAPMWDPATQLRHRKKSSVPPRPDGAPNAFPSVVRVPAREGSAAG
                     SAEAA"
     gene            8544..9878
                     /gene="bioF"
                     /locus_tag="VF517_08520"
     CDS             8544..9878
                     /gene="bioF"
                     /locus_tag="VF517_08520"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX8053024.1"
                     /translation="MSDIGAVLDDLRERGLHRRLRMVSGPQGPRVLLDGKPVLLLCSN
                     NYLGLADHPRVREAAADAAMRWGVGAGGSRLVSGNMTCHRRLEEQLARFKGYQSALLF
                     GSGYLANSGIVAALAGEGEVVFSDELNHASIVDGCRLARAETFVYDHCDIDHLEWGLQ
                     QAGGRGALIVTDSVFSMDGDIAPLPELVELAEHYDVRVMVDEAHATGCLGPGGRGAVA
                     DAGLEDEVDVLVGTLGKALGSYGAYACCDRDIAELLVNRARTLIFSTALPPPAVAGAM
                     AALELLIEQPDRADKLQRNGALLRNALAAEGVPAGESETQIVPLVVGDAAEAMNACEL
                     ALERGVFAQAIRPPTVPQGTSRLRLAVMASHTEAELRRAASVLASALRAGAAPLAAPA
                     PRPSEDPWAWGTGPRQAARAQASAAEPRTSDGDEVGARADQPRVFDGFADAA"
     gene            9930..10598
                     /gene="bioD"
                     /locus_tag="VF517_08525"
     CDS             9930..10598
                     /gene="bioD"
                     /locus_tag="VF517_08525"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX8053025.1"
                     /translation="MRGVFVTGTDTGVGKSVLAAVICAGLAAAGERVAAFKPVVTGLD
                     DPPGEFPPDHELLASVTGQRPQEVAPLRFGPAVSPHLAAELSGVAIEPAALVAAAHAA
                     ARGADSFVCEGVGGLLVPLAPGYVVRDLAVDLGLPVVIAARPGLGTINHSLLAIEAAR
                     GAALDVRAVVLTPWPGDPSAMERSNRETIAALGSVEVATLGVTSTTAFRDAGSALPVS
                     AWIS"
     gene            complement(10655..11170)
                     /locus_tag="VF517_08530"
     CDS             complement(10655..11170)
                     /locus_tag="VF517_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053026.1"
                     /translation="MAKRPLIVVGVMAAFALVPGSAGAAVGDYSCDIASLQVQNEFDN
                     GSSGTFTATGSGQCWTSDPTKKVATQFSASGTYRAQKCSLISVTQPSYLVLNGTLTIT
                     PSGQTAGSTGMTISTADVVTANHSAGTITLASGQTGPVQVDYASPVLGVIARCGGDPF
                     SPEYSGTFLAT"
     gene            11264..11851
                     /locus_tag="VF517_08535"
     CDS             11264..11851
                     /locus_tag="VF517_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053027.1"
                     /translation="MPLSRREFIAGTAFASAAVLLGACSDEEEPTARGTELSPTPACA
                     DDHDATPEQTEGPYYTPDTPKRTNLVERGVTGDRLVIAGTVLGTDCKPIANALLDFWQ
                     ADGDGEYDNEGFRLRGHQFTDRRGRFSLTTVVPGLYTGRTRHVHVKVQRPHGEVLTTQ
                     LYFPGEAQNAQDGIFEEALLMEVQGSRARYDFVLA"
     gene            11848..12426
                     /locus_tag="VF517_08540"
     CDS             11848..12426
                     /locus_tag="VF517_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AhpC/TSA family protein"
                     /protein_id="HEX8053028.1"
                     /translation="MNLRTFLPPLPVPTAAPPEPGEPARELPAPGLDPDRPAVVAFLR
                     HTGCPFAEATARMLREAAAAEPGVAFAAVSHAPTDPTARWCDAIGGLADVRVLIDDGR
                     VHYATWGLGRTSLGHFAGRRSMTEVARLARAGTRNRHPDGTRWQRAGTFAIDAGGTVR
                     WRHVPRHAGELPDLAAALAALRAASGLRAGSA"
     gene            complement(12464..14275)
                     /locus_tag="VF517_08545"
     CDS             complement(12464..14275)
                     /locus_tag="VF517_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8053029.1"
                     /translation="MKWLAPLAACALALALPAVAGASPDAPFGHACTAQNGVRFCPTA
                     TDEQRVASFDGVPIDVDVTLPATGAGPFPTIVMLHGFPGTKESFEATTREGKDAHTYH
                     WNSNYYATEGYAVVTLSSRGFGRSCGVPDSRTSPQCDRGWIHPVADQRFELRDVQHLL
                     GLLADEGVTDPARIGVTGTSMGGSAAMQLGFLRDKVRNADGSLAAWTSPAGKPLAIAA
                     SHPRWGVSDLEYSLVPNGRALDYKTPKHAEGLSPLGVPKASLLNALALGGRLGYVAPR
                     GADPTADLDSWVEALNAGEPYGGDVRALAEQFAAFKGTPRLDAGIAAPLLVQVGWTDP
                     VFPAIEAVRAYQRMNTAVEGGADVAIQIGDVGHFTGGNPLGQYMVFNNQASEFFARLL
                     KIEGSEPAPGQVTAYLQGCPRGSKGSGAIHRNGWPSLGRGLFVMKRQSGTVTSAGGDD
                     ATARTVDPVATSDRCTEVAPGSSKGTTVLTRRSPGFTMLGLPLVRTTVKTKGKYGQLD
                     ALLWEVLPGGKQRIVDFGVYRLTENQKGRIVFQLQGNGYKFRKGSTVKLELRGRTPGL
                     YRPSNGKFSVKLAGTSVEIPTNDGASKSKGIIRPRGG"
     gene            14324..15622
                     /gene="bioA"
                     /locus_tag="VF517_08550"
     CDS             14324..15622
                     /gene="bioA"
                     /locus_tag="VF517_08550"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX8053030.1"
                     /translation="MSLAADDRRFIWHPFTQQRGWEHEEAPTIERADGVHLYDSDGKR
                     YIDGVSSLWCNVHGHRHPVIDIAVKDQLDHVAHTTMLGLSHPTAVELAKRLVELAPDG
                     LARVFYSDSGSTATEIALKMAFQFWQQQGGEHARRKRFVCLRMSYHGDTIGSVSVGGI
                     DLFHSMYRPLLFDALRAEPGDAADMERVLAEHGDEVAAVIVEPLVQGAAGMLIHPEGY
                     LRDVRELCDRHGTLLICDEVATGFGRTGRMFACEHEGVTPDLLCLAKGLTGGYMPLAA
                     TLATERIYEGFLGEFEEFRTFFHGHTYTGNPLACAAALASLRLFEQERTLEALEPKID
                     LLGRLLEPVAALPAVGEVRRRGFMTGIELSGFPVEARIGHQVTLEARRRGAIVRPLGD
                     VVILMPPLSIKAAELRELVEITAESIEAATASRVQLAAAA"
     gene            complement(15635..15811)
                     /locus_tag="VF517_08555"
     CDS             complement(15635..15811)
                     /locus_tag="VF517_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX8053031.1"
                     /translation="MEAATQQMWICESCGFIYDPAEGDPDGGIPAGTTFEAIPDDWMC
                     PVCGARKKDFVPYE"
     gene            15850..16470
                     /locus_tag="VF517_08560"
     CDS             15850..16470
                     /locus_tag="VF517_08560"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8053032.1"
                     /translation="MARIDRPPPGEELNTGATTQARLAATAMLVRDGTEGLEVLLVQR
                     NPEQRFMGGAWVFPGGAVHDDDADPESAAVRELEEEAGLRIEDVRELVPFSRWITPVE
                     VKIRFDTFFYLAAAPPDAEARVDGAECVDARWIRPADALDAGTRGELMLVFPTIKHLE
                     QLAELESVEHALETARSRQVEPILPKVVYGEGEAHVLLPGEPGYPD"
     gene            16532..17302
                     /locus_tag="VF517_08565"
     CDS             16532..17302
                     /locus_tag="VF517_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8053033.1"
                     /translation="MALALAAVLALMGGTLAIADTLLGDAGPNELTGTAEPDGLWGEE
                     GDDKLTGLAANDYLEAGPGSDKVDAGPGDDFATGGSGEDELKMGSGRDVGYAGPGNDS
                     VLGEDGDDVLLGQLGDDRVFGGPGNDRLYGGTGNDFVDGGTGNDQLFADVGINTMLGG
                     AGNDRFMVQGGATSNIEGGSGDDKVSLSSGDDKVDGGGGDDRISTGSGDDTVDTVDGR
                     RDVVKCGKGTDTVRADKIDRLTDCENVTRASPKGTRRR"
     gene            17331..18368
                     /locus_tag="VF517_08570"
     CDS             17331..18368
                     /locus_tag="VF517_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8053034.1"
                     /translation="MAQQGLTVAITGPTGDLGIAMVEALERSRRVKRIVGMARRSFDP
                     KDRGWRKTEYRQGDVQDAASVKDAVKGADVVVHLAFAILTAGNASRGINVDGSKNVFE
                     AAVAAGAERICYASSVAAYGFHDDNPEWLTEDIPPRGTPEHPYSAQKAEVEQILNAAL
                     ARSSRTSAWVFRPCIVAGPKATTFLEEIPYFRLSERMPDPVRRLVGGMPMLKPVIPDT
                     GPPFQLVHEDDVASAFVAGVHGRGSPGPYNLAGRGKLTLSDVADALGWYSVPFPDLAL
                     EATMEVLARLPRLPEQAAWIQSARRPVLMRTTRAQKELGWRPKHTARATLKQMIEAYR
                     SEQAGVEAGAA"
     gene            complement(18375..19565)
                     /locus_tag="VF517_08575"
     CDS             complement(18375..19565)
                     /locus_tag="VF517_08575"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX8053035.1"
                     /translation="MTAFKDFAQHIVRTRVVAGHGLLEGVGFELMKEGAERVFVVTDQ
                     VIRGTGLVDRVEAGVTDGGLELAGSFDDVPQDSGVEVVARCAEEARAAGADSFLAVGG
                     GSVMDTAKAAAVLFTHGGTVHDYEGLYALPRANDGMGQALPVAPVACVPTTAGTGSEV
                     SMAVVVKDHEAKVKLELADVPLFPRLAVLDPECTRTLPPAIAAATGMDAMTHAIEAYV
                     TVESSPFSDAEALHALRLIRDNLERAVHGGAGDDEARGNMLVAASLAISVYLGSTHAM
                     SHACGAHFGVAHGVANAINLPHVIRFNAAGGAEIADRYRDLAELLTIEAGGSDEAVGD
                     ALATHVGSLVSGLGLPTRLSEVGVPEDGIPALIEGAMGDGTTLMNPREPTEEDYERLF
                     RAAL"
     gene            complement(19562..19981)
                     /locus_tag="VF517_08580"
     CDS             complement(19562..19981)
                     /locus_tag="VF517_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8053036.1"
                     /translation="MAYFKDADEVYAHIGKLFQDLVTDDELAAKFRKANATVQYQYRN
                     PESQITVRLREDVEPQVDLGESDLDPDVVMTMDADTAHRFWLGKVNVTVALARGQMKA
                     KGPVAKVLKLVPLVKPVFPRYRQQLVDAGRQDLADAA"
     gene            complement(20019..21212)
                     /locus_tag="VF517_08585"
     CDS             complement(20019..21212)
                     /locus_tag="VF517_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8053037.1"
                     /translation="MRRALLLLALIAAIATLAACNGDEREETLSAEPVAAVAEAPARA
                     AASSRLRLARIGTFDQPTYVTAPPGDKRRVFVTEQPGRIRVLVDHKLRSRAFLDIRGE
                     VSCCGERGLLSMAFAPDYAKSGRFYVYVTGDDGDIRVLQFRRSSNAQLADPGSGREVI
                     RIEHSQFGNHNGGQLQFGPDGMLYLATGDGGGGGDPLRSAQDLGSPLGKLLRIDPRPG
                     GGYDIPADNPYRDRSGARGEIWARGLRNPYRFSFDRKGGGLAIGDVGQNEFDEIDYPP
                     KRGRAANFGWSVFEASSRYREGSVSGHVKPVLVKRLRENGNCATIGGYVVRDPALRAL
                     AGRYLHGDNCNPTIYSMRISTKGASGNRSTGLRLSGLSSFGEDALGRIYVTSLSGGVY
                     RLVAR"
     gene            21275..>21718
                     /locus_tag="VF517_08590"
     CDS             21275..>21718
                     /locus_tag="VF517_08590"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8053038.1"
                     /translation="MKALVTGATGFVGGRLVEALAERGADVRCLVRDAERAQHLADAG
                     HELHEGDVLKPETLAGAGEYVDVAYYLVHSMGRGSGDGFEERERRAARSFAEMASREG
                     VERVIYLGGLGDEPESEHLRSRHQTAAILSEHGPPLTYFRAAMVVG"
BASE COUNT         3168 a         7774 c         7671 g         3105 t
ORIGIN      
        1 cgggccgatc ctcatcgaca ccggcctgca cccctcggtc gcggtcgaca agaagcagaa
       61 cctcgggcgc ttcttcgcct ccaccgtcgc gcgctcctgg gagatggacc ccgatcaggc
      121 gctgcccgac cagctccgcg agcgcggcat cgagccgtcg gacgtgaggc tggtcgtgat
      181 gacccacctc cacctcgacc acgcgagcgg catggtccag ttccccgagg ccacgttcgt
      241 gatcagccgc caggagtggg agcgcgcgag cgagccgcgg gcggtcacga gcggctacgt
      301 cacgagccac ttcgaccacg ccttcgacta ccggctgctc gacttcgacg accccggcgt
      361 cggctcgttc gcgggcttcg gccgctcgtt cgacctgctc ggcgacggca gcatccacgt
      421 ggtcttcacg ccgggacaca cgctcggtca catgtcggtg gtcctgcgcg gcgacgagcg
      481 cgaggtcctg gtggcgggcg acgcgatcta cacgatgcac gccctgaaga ccggccacaa
      541 gccgtacctg atgcaggaca gccacctctt cgggcgctcg gtgcgcgaga tccagcgcta
      601 cgtggagcag acgccgagcg cgatcgtcgt gcccgggcac gagatggagc cgttcgagga
      661 ggctcggagg gcgctggccc gctagccggc ggcggtgcgg cgcgccgcgt tgcccgcggc
      721 cttgcccgcg gcgcggcgcg cggcgcgggc ggtctggatc gcgttgcggc ggccgccgcg
      781 gcgcagcccg tagaacacct tctgggtgcg cgcgaagatc tcgttggcgg cggcctgtgc
      841 ctcgtcgcgc gtcggctcct ccacggtgcg ctcgaacacg atcggatcgc cgtactggac
      901 ggtgaccttc gggaagcgca gccgcttcca gttgcgcgcg ccctcggagc cggcgatggc
      961 ggacgggacc accacggcgc cggtctcgag cgccagccgc ccgatgcccg gcttggcctc
     1021 ccccagctcg ccggtgcgcg agcgcccgcc ctcggcgtac atgacgatca ccttgccgcg
     1081 cgagagcagc gagtgcgcgg tcttgaacat ctcctcgtcg cggtggccgc gacggaccgg
     1141 gaacaccccg ccgtgggtgt agatgaactg gagcggtcgc ttgaagagct gcgacttcgc
     1201 cacgaagtgg accttgcgcc gcacgtacac cgagatcagg aagtggtcca tgaacgagaa
     1261 gtggttgggc gcgatgatca ccgacccctc cggcgggatc ttctgcgagt cgatgcagcg
     1321 ggtcctgaag aagaagagca ggaccggcgt gagtatgagg cgcaccacct cgtacatgaa
     1381 gtccggcttg cgcgtgcgcg tgcgctcgtg gaagcgggtg aagtactcgg ccggccgcgg
     1441 gtccttgtag acctgcggct tcatgggggc tagatcctgt tcgggcatgg gggtggagag
     1501 agctacccag ctctttcgcg gacgttaccc cgcgtccgct tcgggagaaa cccaggcgcg
     1561 cgagcgctcg accgcgcgcc gccactcggc gagcagcgat tcgcgctcgt cagcggtcat
     1621 gcgcggctcg tagcgcgcgg cctcccgcca catctggcgc gtgcccgcct cgtcccagaa
     1681 gccggtggcg accccggcga ggtacgccgc gccgagcgcg gtcgtctccg agatctccgg
     1741 caccacgacc ggcacgccga gcacgtcggc ctggaactgc atcagccagg cgttcaccgc
     1801 cgccccgccg tcggcgcgca gctcggccag cggcgcgctg gaggtggcct cgatcgcgcg
     1861 cacggcgtcc acggtctggt acgcgatcgc ctcgagcgcg gcgcgggcca ggtgcgcgcg
     1921 gccgttgccg cgcgtgaggc cgacgatcgt cccgcgcgcg tatggatccc agtgcggcga
     1981 gccgaggccg gtgagcgccg ggacgaagta cacgccgtcg ttcgagtcga gcgagcgagc
     2041 cagctcctcg gtctccgcgg ccgcctcgat gatcccgagg ccgtcgcgca gccactggac
     2101 cgccgcgccg gtgacgaaca cgctggcttc gaccgcgtag tcgacgcggt cccccacccc
     2161 ccaggcgatc gtgttcagca ccccctcctg caccggcggc gcctcggtgc cggcgttctg
     2221 gagcacgaag ctgccggtgc cgtaggtgtt cttgccgagg ccgggctcgc tgcacgcctg
     2281 gccgtagagc gcggcctgct ggtcgcccgc gatcccggcc accgggacgc tcccgccgag
     2341 cgcgtcggtc tcgccgtaca cgtgcgcgct cgggagcacc tcgggcagcg aggcggggtc
     2401 gaccgacagc agctcgcaga gctcgggatc ccacttgcgc gagcggatgt cgaacagcag
     2461 cgtgcgcgag gcgttcgtgt agtcggtcac gtggcggccg gtgagcttgt ggagcagcca
     2521 cgagtcgatc gtcccgaacg ccgcccgggc ggggtcgacg ccgccctcgc gcacgagcca
     2581 ctcgatcttg gtgccggaga agtaggggtc gagcacgagc ccggtgcgct cgcggaaggt
     2641 gggctcgtgg cccgcgtcgc gcagctcgtc gcagcgggcg gccgtgcgcc ggtcctgcca
     2701 cacgagcgcg cgatgcagcg gctcgccgct gtcgcggtcc caggcgacga cggtctcgcg
     2761 ctggttcgtg atgccgatgc ccgcgagctg cccggcgtct gtccccgcgt cggcgagcgc
     2821 gtcgcgcgcc acgccgaggg tggtgtccca gatctcctcc gcgtcgtgct cgacccagcc
     2881 cgggcgcggg aagtgctgct cgaattcgct gtaggcgcgg ccggctggct ggccgtcggc
     2941 gccgaacacg atgcaggtcg tgcccgtggt gccctggtcg atcgcgagga tcatcctctg
     3001 tccctccccc ggttcaagcg gcgcagagcg taaacccgga cgaagtcgcg cagctgcctg
     3061 ccgcggtggg cgaagcctgc ggcggtgcgg ccggtggcgc gatgctcgag cggcagctcg
     3121 acctcgacga cccggaaccc ggcccggtgg gcgtcgacgg tcatcccgat ctccatgccg
     3181 aagcgcgggg cgaacgggag cacggcgtcg agcagcgcgg gcgcgagcgc gcgctggccg
     3241 ctgatcggcg cgcgcaggtc gaggccggtg agcgaccgga tcgcgcggtg ggcgaagccg
     3301 acggccagcc cgaacccgcc gccgacgcgg gtggcgaaga ccgccacggc gagatcgccc
     3361 tcgccgcgct cgacggcggc gaccagggcg ggcaggtggc gggcggagcc accgaggtcg
     3421 ccgtcacaga ggacgagcag cggggcggag ccgccgcccg gggcggagcc gccgcccggg
     3481 gccgcgccgc cgcccggggc ggagccgcca cccggagccg agccgccgcc gcgcgcccgc
     3541 tccgccgcca gcgtcgccac gccgcccttc ccgagccggc gcggcgcggt caccgcctcg
     3601 gctccggccg cgcgcgccac gtccgcggtg ccgtcgtcgg acgcgtcgtc ggccaccagc
     3661 acgcgcgcgc ccggaaacgc ctcgccgagg gcggacaggg tctccccgag ccggtcggcc
     3721 tcgttgtagg cggtcacgac gatcgctatt tcgggccgca cgaccggtta tcgtgcccga
     3781 cgcaacggac ccgcgaccag gaggagcagt tgcccgacgt cgaagcgcac atcaccggca
     3841 ccgtctggaa ggtcgagtgc aaggtcggtg acaccgtcga agagggcgac accctggtga
     3901 tcctcgagtc gatgaagatg gagatgcccg tcgaggccga ggacgacggc acggtgtccg
     3961 agatccgctg cgaggaaggg cagtcggtgt cagagggcga cacgctcgtt gtcctcgagt
     4021 agcgaaggga tggaactcgc gtcgggcaag ctcgtcatcg acgagccggc gtcgaacgtc
     4081 gtcaggatca ggatcgcgaa ccccgacaag cgcggggcgc tcgaccacga gatcctcgac
     4141 gcgctcgcgg acaccgtccg caacctcgac tgcctctgcc tggtcatcac gggcaccgcc
     4201 ccggtgttct ccgccggcta cgacatcggc aacttcccgg agtccacctt cgacggcagc
     4261 ggcggcgatc gcggcttcgc cgacgccgcc gagaagctcg tcgcgcaccc gttcacggag
     4321 gcgatcgagg cgatcgaggc ctacccgtac ccggtggtgg ccgcgatcaa cggccacgcg
     4381 atcggcggcg ggctcgagct ggccgtcacc tgcgacgtgc gcatcgccgc gcgcggctcc
     4441 cggctcggga tgccgccggc gaagatcggt ctgatctact cgtacacggg gctgcgccgc
     4501 ttcatcgaca tctgcggcgt cggcaacacc agcgagctct tctacacggg ccgcaacgtc
     4561 gaggccgacc acgcgtacga gatgggcctc gtgaaccgcg tcgtagaggc ggacgagctc
     4621 gaggaggccg cgctcgccgt agcccgcgag atcggcacga actcgccgct ggcgctgcgc
     4681 gggaacaagg aggtcatgcg cacgctgcgc gcccacagcg gcgacctccc ggcgatcgag
     4741 gcccgccgcc tcgtcacgct gcgcgagtcg tgcttcacct cgggggactt catcgagggc
     4801 atccgcgcct tcggcgagaa gcgcgccccg cgctggtccg gccgctagcg gtccctagac
     4861 tcggcgatca gtagagcgtc ggcgaggagg acgagtgcga gctgccggat tttggagcta
     4921 cacccgcgaa gatgatgtgg gcgacaacgg ccgcgtcgcg cggctggccg accagctcaa
     4981 ggccgagtat gggctgatca caggcgacga actcgaactc ttcgtcgacc ggacgtcgct
     5041 cgactggggc gaccagtggc gcacacgaat tgacgaagca ctggcgacga cgaccttttt
     5101 tatccccgtg atcactccgc gatatttccg gcggccggaa tgccgcaagg aattgcttac
     5161 attcgccggc cacgcccaga gcctgggcgt agaagagttg ttgctcccgt tgctctacgt
     5221 agaggtgcct gacctcgatg aagactccga agatgaggcc attgcgctag ttgcaggcac
     5281 ccagtacgag gattggcgaa accttcgctt ggtcgacgag aactcggccg attaccgtca
     5341 gggtgtaaat cgcttggcaa cccgtctcgc aaacatcagc gaagaggttg cggaccggcc
     5401 cgacccgttg ccgccccagg gtagatcgac agctgacggc gatgccgacg aagggccagg
     5461 gtttcttgag cttcttgccg agggcgaaaa ggcgttgcct cggtgggagc gcacaatcac
     5521 cgcgttcccc gacgtgcttg aggaagtcgc gacggtgacg gagggcgcta ctgaccagat
     5581 gtctgagagc gatgctcgcg gaggcggatt cgcggcccgc atagcaatca cacgccgtct
     5641 cgccagagaa ctcgatgctc ccgctaagcg aatcgctgaa ctgggacggt cctacgcctc
     5701 agacttagta gctatcgatc ccgccattct gacgcttctg cgttgggcag aagaggatcc
     5761 agatgaagcc gcgacgcccg aggctcaaga atttttcaca tctatacagg ccctcgcaca
     5821 ggcgtcgaag gaggccacca aagagctgaa ggccttcgtt acagcgttag atgacgcctc
     5881 acaaatgacg cgagaaatcc ggccccaagc tcgcactatg cgcgagggct tgcgcgacat
     5941 ccttgacggt caagccgtga tcgacgaatg ggaacgacgc gtgagccgag ttacggctgc
     6001 agtggaacag cagcacaccg cgcatgagta gcacgtcggc atagtcccac gtacggcgtg
     6061 agcgctcggg gcagacggcg actacttgag ttcgttcgca cacgtacggt ccgagcggtg
     6121 cttttcgccg cagtagtgat tttcgccgta gtgctggcac tcatcgttgt ctttgccgtg
     6181 ctcccaaact tggtcgtctc tgacagcgcg gtgaaagcgg aggtcgaccg cgtgaagctc
     6241 gaaaacgacg tacgacgcac cggcgcacag atattggcgg ggctcttggt cctcggagga
     6301 ctgtttctga cagcgcgctc catccgcgtc acccgggagg ggcaattgac agaacggttt
     6361 tcgcgggcca tcgaccagct cggacacgag gaactcgacg tgcggctcgg cgggatctat
     6421 tccttagagc gaatcgcgcg cgattcagct tccgatcgcg gaccggtgaa tgaggtgctg
     6481 agtgcctggt tgcgtcgaaa gtcgcgcgtg cgtcggcggg gcaccggacg tcgactacct
     6541 gaagatgttc gcgccgtgct cgtggtgctt ggacgccgac cgaaggcgcc gagctcgaac
     6601 gcagatcggg tcgacctcac cagtatcgac ctgcgcagtg cggaccttct ggtcctcgat
     6661 ctaccccgcg cggtcctctc cgacgcccgg ctcgacggcg ccgatctgac gagggccaag
     6721 ctaaatcagg ctcacctcac ccgagcgcat ttcgagaacg ccgacctcac tggtgccgag
     6781 ctaaaaaagg ccacactcac gaacgctcgc ttcgatgccg cggacctgtc cgagacgacg
     6841 ttgaccaaag ctacgtgtac aagtgggtcc ttcatcgacg caacgctcgc atcgtcggtc
     6901 ctgtcaaggg ctgagttcgc tcacgccgac ctttcgcgca gtgatttgca gggcgcgaaa
     6961 gccaggaaga ccaacttcgc tcgcgcgaga ttggcggggg caaatcttac gcgtgcggac
     7021 gtacgcggcg cttcattctt aggggcgtca cttcgtggcg caaacctttc cggcacgacg
     7081 ctcaaacgag cagatcttcg aggcgccgac cttcggaacg cagccggcct aacggccgag
     7141 caggtcgcgg tggctcgctg tgacatgact acaaggtttt ccgctcccga cccagctacg
     7201 cgacaggact agcgggcctg actgatcgca ctcggctagt gggctctccc cagcccacga
     7261 aggggggagg ttcgcgcagt tagagggatc aaagcgtccg acccccaccg taccttgggg
     7321 cttgctcgtc cccacgagga ggtcaaccgc ttgccgccgc taatcagcta cgacgaggcg
     7381 ctcgccctcg gcgagatcga ggaccacgcc gagatcgagg cgctcgtgga ccgggccttc
     7441 gcggcgcggc tcgagcactt cggcgactcc accgacatgt gctcgctcgt caacgcgaag
     7501 tccggcggct gcgccgagga ctgcggcttc tgcgcgcaat cgaagtccgc ggagtcggac
     7561 acgccgatgc acgcgatgat gaccccggag cagatcctcg agcacgcgcg cgcggccgag
     7621 gcggcgggcg cgcaccgctt ctgcatggtc acgcaggggc agggactgtc gaaaaaggac
     7681 ttccagaacg tcctcgacgg cgcgaagctc gtcgccgagc acaccaacct caagcgctgc
     7741 gtctcggtcg ggcacatctc gcgcgaccgt gccaaggcac tcaaggaggt gggcatccag
     7801 cgcgtccacc acaacgtcga gaccgcggag agctactacc cggaggtctc gtcgacggtc
     7861 cgctacgagg gccgcctgcg cacgatcgac gcggtggcgg acgccgggct cgagacctgc
     7921 gtcgggggga tcctgaacct cggcgagagc cgcgctcagc gggtcgagat ggcgttccag
     7981 ctcgcgaagg tggatccgac gagcgtgccg atcaacctgc tcaacccgcg tcccggcacg
     8041 aagttcggag accgcgactt catggacccg tgggaggcgg tcaagtggat cgcgatcttc
     8101 cggctgatcc tgccgaaggc gctgttccgc ctctgcggcg gtcgcgtgga gaacctcggc
     8161 gagctcgagc cgatggcggt caaggcgggg ctgaacggcg tgatgatggg caacttcctc
     8221 accactctcg gctccgagcc cgaggacgac cgcgcgatgt tcgagtcgct cgggctgaac
     8281 acggcgcgcc agccagacaa cggggccaac ccgcggccgg acaaccgctc cggctggctc
     8341 gagggcgact cgccgagcac gccggtggac cggctgatcg accgccagga cgaggcgccg
     8401 atgtgggacc cggccacgca gctgcgccat cgcaagaagt cgtcggtgcc gccgcggccc
     8461 gacggggcgc ccaacgcgtt cccgagcgtg gtgcgcgtgc cggcgcgcga gggatccgcg
     8521 gcgggctcgg ccgaggccgc ctgatgagcg acatcggcgc ggtcctcgac gacctgcgcg
     8581 agcgcggcct ccaccgccgc ctgcgcatgg tctcggggcc gcagggcccg cgcgtgctgc
     8641 tcgacggaaa gccggtcctg ctgctgtgct cgaacaacta cctcggcctg gccgaccacc
     8701 cgcgcgtgcg cgaggcggcg gccgacgcgg ccatgcgctg gggcgtcggc gccgggggct
     8761 cgcggctcgt ctcgggcaac atgacctgcc accgccgcct cgaggagcag ctcgcgcgct
     8821 tcaagggcta ccagtccgcg ctgctgttcg gctccggcta cctggccaac tccggaatcg
     8881 tggccgcgct cgcgggcgag ggtgaggtcg tgttctcgga cgagctaaac cacgcgagca
     8941 tcgtggacgg ctgccgcctc gcgcgcgctg agacgttcgt ctacgaccac tgcgacatcg
     9001 accacctcga gtgggggctc cagcaggccg gcggccgcgg cgccctgatc gtcaccgaca
     9061 gcgtcttctc gatggacggc gacatcgccc cgctgccgga gctcgtggag ctcgcggagc
     9121 actacgacgt gcgcgtgatg gtcgacgagg cgcacgcgac cggctgcctc gggccgggcg
     9181 ggcgcggcgc ggtggccgac gccggcctcg aggacgaggt ggacgtgctc gtcggcacgc
     9241 tcggcaaggc gctcgggtcc tacggcgcct acgcctgctg cgaccgcgac atcgccgagc
     9301 tcctcgtcaa ccgggcccgc acgctgatct tctcgacggc gctcccgccg ccggccgtcg
     9361 ccggggcgat ggccgcgctc gagctgctga tcgagcagcc ggatcgcgcc gacaagctcc
     9421 agcgcaacgg ggccctgttg cgcaacgcgc tcgccgccga gggcgtgccc gcgggcgagt
     9481 ccgagacgca gatagtgccc ctggtggtcg gcgacgcggc cgaggcgatg aacgcatgcg
     9541 agctagcgct ggaacgcggc gtgttcgcgc aggcgatccg cccgccgacg gtgccccagg
     9601 ggacctcccg gctgcgactg gccgtgatgg cctcgcacac ggaggcagag cttcggcggg
     9661 cggcgagcgt gctggccagt gcgctacgcg ccggcgcggc ccccctcgcc gctccggcgc
     9721 cccgcccgtc ggaggatcct tgggcctggg gaacgggccc gcggcaggcg gcgcgagcgc
     9781 aggcgtcggc cgccgagccg cgcacgagcg acggcgacga ggtcggcgcg cgcgccgacc
     9841 agccgcgcgt cttcgacggg ttcgccgacg ctgcctgact gggcgacatt cgccccatat
     9901 agcgaggtca agatggccca gctcgcgtaa tgcggggcgt cttcgtcacc gggaccgaca
     9961 cgggtgtcgg caagagcgtc ctggccgccg tgatctgcgc ggggctcgcc gccgccggtg
    10021 aacgggtggc cgccttcaag ccggtggtca caggtctcga tgacccaccg ggcgagttcc
    10081 cgcccgacca cgagctgctg gcctcggtca ccgggcagcg tccgcaggag gtcgcgccgc
    10141 tgcgcttcgg gcccgcggtc tcgccgcacc tggctgcgga gctctccggc gtggcgatcg
    10201 agccggcggc gctggtcgcc gctgcccacg ccgccgcccg cggcgccgac tcgttcgtgt
    10261 gcgagggcgt cggcgggctg ctggtgccgc tcgcgcccgg ctacgtggtg cgcgacctgg
    10321 cggtggacct cgggctgccg gtcgtcattg ccgcgcggcc gggactcggg acgatcaacc
    10381 actccctgct ggcgatcgag gccgcgcgcg gggcggcact ggacgtgaga gccgtcgtcc
    10441 tcacgccgtg gcccggcgac ccgagcgcca tggagcgctc gaaccgcgag accatcgccg
    10501 ctttgggctc ggttgaggtc gccaccctcg gggttacgtc gacgacggca ttcagggacg
    10561 cgggatcggc gcttccggtc tccgcgtgga tctcgtaatc gtcgtgcttt cctgacttat
    10621 gggtctcctg cgcccataag tcaggaatga tcgatcaggt cgccaggaac gtgccgctgt
    10681 actcggggct gaacgggtcg ccgccgcagc gagcgatcac gccgagcacc gggctcgcgt
    10741 agtccacctg cacggggccc gtctggccgc tcgccagcgt gatcgtgccg gcgctgtggt
    10801 tcgcggtgac cacgtcggcg gtcgagatcg tcatgccggt cgagcccgcg gtctggccgc
    10861 tcggcgtgat ggtgagggtg ccgttcagga cgagatagct cggctgcgtc accgagatga
    10921 ggctgcactt ctgcgcgcgg taggtcccgc tcgcgctgaa ctgggtggcg accttcttcg
    10981 tcggatcgct cgtccagcac tggccgctgc cggtggcggt gaacgtgccg gagctgccgt
    11041 tgtcgaactc gttctgcacc tgcagcgagg cgatgtcgca cgagtagtcg cccaccgcgg
    11101 cgccggccga acccggaacc agcgcgaacg ccgccattac acccaccacg atcaacggtc
    11161 tcttcgccat ctcgtccccc ttagcgcttg aaagtcagcc ttccgcatac ccccggtcac
    11221 agctcttaca cccgtcttac ggcggcgacc tacgctcgct cgcatgccgc tctcgaggcg
    11281 tgagttcatc gcggggaccg ccttcgcctc cgccgccgtg ctgctcggcg cctgctcgga
    11341 cgaggaggag cccaccgcgc gcggcacgga gctgagcccc acccccgcct gcgccgacga
    11401 ccacgacgcc acgcccgagc agaccgaggg cccgtactac acgcccgaca cgccgaagcg
    11461 gaccaacctc gtggagcgcg gcgtgaccgg cgaccggctc gtgatcgccg ggaccgtgct
    11521 cgggacggac tgcaagccga ttgcgaacgc gctgctcgac ttctggcagg ccgacgggga
    11581 cggcgagtac gacaacgagg gcttccgcct gcgcgggcac cagttcaccg accggcgcgg
    11641 gcgcttcagc ctgacgacgg tcgtgccggg cctctacacc ggccgcaccc gccacgtcca
    11701 cgtgaaggtg cagcgcccgc acggcgaggt cctcaccacg cagctctact tccccggtga
    11761 ggcgcagaac gcccaggacg ggatcttcga ggaagccctt ctgatggagg tgcagggatc
    11821 gcgcgcgcgg tatgacttcg tgctcgcgtg aacctccgta cgttcctccc cccgctgccg
    11881 gtcccgaccg ccgcgccgcc tgagcccggt gagccagcgc gcgagctccc cgccccgggg
    11941 ctcgatccgg accggcccgc cgtggtcgcg ttccttcgcc acaccggctg cccgttcgcc
    12001 gaggcgaccg cacggatgct ccgcgaggcg gccgccgccg agcctggcgt cgcgttcgcc
    12061 gcggtgagcc acgcccccac cgacccgacc gcccgctggt gcgacgcgat cggcggcctc
    12121 gcggacgtgc gcgtgctgat cgacgacggc cgcgtgcact acgccacctg gggcctcggc
    12181 cgcacgagcc tgggccactt cgccggacgc cgctcgatga ccgaggtcgc gcgcctcgcc
    12241 cgcgcgggca cccgcaaccg ccacccggac gggacgcgct ggcaacgcgc cggcaccttc
    12301 gccatcgatg ccggcggaac ggtccgctgg aggcacgtgc cccggcacgc gggcgagctg
    12361 cccgacctcg ccgcggcgct cgccgcgctg cgcgccgcct ccggcttgcg cgccggctcg
    12421 gcctagccgc ggcgctcgcc gcgctgcgcg ccggctcggc ctactagccg ccgcgcgggc
    12481 ggatgatgcc cttgctcttg ctggcgccgt cgttcgtggg gatctccacg gacgtcccgg
    12541 cgagcttcac gctgaacttc ccgttcgacg gccggtacag ccccggcgtg cgcccgcgca
    12601 gctcgagctt cacggtcgag cccttgcgga acttgtagcc gttgccctgg agctggaaga
    12661 cgatccgccc cttctggttc tcggtgagcc ggtagacgcc gaagtcgacg atccgctgct
    12721 tgccgcccgg gagcacctcc cagaggagcg cgtcgagctg gccgtacttg cccttggtct
    12781 tgacggtcgt ccgcacgagc ggcagcccga gcatcgtgaa gcccggcgac ctgcgcgtga
    12841 gcacggtcgt gcccttcgag gagcccggcg cgacctcggt gcagcgatcg ctcgtcgcga
    12901 ccgggtcgac cgtcctggcg gtcgcgtcgt cgccgcccgc ggaggtgacc gtgccgctct
    12961 gccgcttcat cacgaacagg ccgcgaccca gggacggcca gccgttgcga tggatcgcgc
    13021 cgctcccctt cgaccccctg gggcagccct ggaggtaggc ggtgacctgt cccggcgcgg
    13081 gctccgaacc ctcgatcttc agcagccgcg cgaagaattc ggacgcctgg ttgttgaaga
    13141 ccatgtactg cccgagcggg ttgccgccgg tgaagtggcc gacgtcgccg atctggatcg
    13201 ccacgtcggc gccgccctcg accgcggtgt tcatacgttg gtacgcgcgc acggcctcga
    13261 tcgcggggaa cacggggtcg gtccacccga cctgcaccag gagcggcgcg gcgatccccg
    13321 cgtcgagacg gggcgttccc ttgaaggcgg cgaactgctc ggcgagcgcg cgcacgtcgc
    13381 cgccatacgg ctcacccgcg ttgagggcct cgacccaact gtccaggtcg gcggtcgggt
    13441 cggcccccct aggcgcgacg taccccagcc gcccgccgag ggccagcgcg ttcagcaggc
    13501 tcgccttcgg cacgccgagc ggcgagagtc cctcggcgtg cttgggcgtc ttgtagtcga
    13561 gcgcgcgccc gttcggcacc agcgagtact cgaggtcgga gaccccccag cgcgggtggg
    13621 aggcggcgat cgccagcggc ttgccggccg ggctcgtcca cgccgcgaga gagccgtcgg
    13681 cgttgcggac cttgtcgcgc aggaagccga gctgcatcgc cgcgctcccg cccatgctcg
    13741 tgccggtcac gccgatgcgc gcggggtccg tcaccccctc gtcggcgagc agcccgagga
    13801 ggtgctgcac gtcgcggagc tcgaagcgct ggtcggcgac cgggtgtatc cagccgcgat
    13861 cgcactgcgg cgaggtgcgc gagtcgggca cgccgcacga gcgcccgaag ccgcgactcg
    13921 agagggtcac gaccgcgtag ccctcggtcg cgtagtagtt gctgttccag tggtaggtgt
    13981 gcgcatcctt gccctcgcgc gtggtcgcct cgaaggactc cttcgtgcct gggaagccgt
    14041 gcagcatcac gatcgtcggg aacggtccgg caccggtcgc gggcagcgtc acgtccacgt
    14101 cgatcggcac gccgtcgaag ctcgccaccc gctgctcgtc ggtcgcggtg gggcagaagc
    14161 gcacgccgtt ctgcgcggtg cacgcgtggc cgaacggagc gtccggcgat gcgccggcga
    14221 cggcgggcag agccagggcc agcgcgcagg cggcgagggg ggccagccac ttcacgcttc
    14281 ccaccctaac cgttccgtcc ctggccgggg gtaccttggc gacgtgagcc tcgcggccga
    14341 cgaccgccgc ttcatctggc accccttcac gcagcaacgc gggtgggagc acgaggaagc
    14401 ccccaccatc gagcgcgccg acggcgtcca cctctacgac tccgacggca agcgctacat
    14461 cgacggcgtc tcgtcgctct ggtgcaacgt ccacggccac cgccacccgg tgatcgacat
    14521 cgcggtcaag gaccagctcg accacgtcgc gcacacgacg atgctcggcc tctcgcaccc
    14581 caccgcggtc gagctggcca agcggctcgt cgagctcgcg cccgatggcc tggcgcgcgt
    14641 cttctactcg gacagcgggt cgaccgcgac cgagatcgcg ctaaagatgg cgttccagtt
    14701 ctggcagcag cagggcggcg agcacgcgcg ccgcaagcgc ttcgtctgcc tgcggatgtc
    14761 ctaccacggg gacacgatcg gctcggtctc ggtcggcggg atcgacctgt tccactcgat
    14821 gtaccgcccg ctcctgttcg acgcgctgcg cgcggagccg ggcgacgccg ccgacatgga
    14881 gcgcgtgctc gccgagcacg gcgacgaggt ggcggcggtc atcgtcgagc cgctggtgca
    14941 aggcgcggcc ggcatgctga tccaccccga gggctacctg cgcgacgtgc gcgagctctg
    15001 cgaccgccac ggcacgctcc tgatctgcga cgaggtcgcc acgggcttcg gccgcacggg
    15061 ccggatgttc gcctgcgagc acgaaggcgt cacgcccgac ctgctgtgcc tggccaaggg
    15121 cctcacgggc ggctacatgc cgctggccgc cacgctcgcg accgagcgga tctacgaggg
    15181 cttcctcggc gagttcgagg agttccgcac gttcttccac ggccacacgt acacgggcaa
    15241 cccgctcgcg tgcgccgcgg cccttgccag cctgcgcctg ttcgagcagg agcggacgct
    15301 cgaggcgctc gagccgaaga tcgacctgct cggccgcctg ctcgagccgg tggccgcgtt
    15361 gccggcggtc ggcgaggtgc gccgccgcgg cttcatgacc ggcatcgagc tgtcggggtt
    15421 cccggtggag gcgcggatcg gccaccaggt cacgctcgag gcgcgccgcc gcggcgcgat
    15481 cgtccgcccg ctcggcgacg tcgtgatcct gatgccaccg ctctcgatca aggcggccga
    15541 gctgcgcgag ctcgtcgaga tcacggccga gtcgatcgag gccgccacgg cctcgcgggt
    15601 ccagctcgcc gcggccgcct gagccgccga ggccctactc gtaggggacg aagtccttct
    15661 tgcgcgcgcc gcacaccggg cacatccagt cgtccgggat ggcctcgaac gtggtgccgg
    15721 cggggatccc gccgtccggg tcgccctccg cggggtcgta gatgaacccg cacgactcgc
    15781 atatccacat ctgctgggtt gccgcctcca cgggcgacag tcttgcaaac gcaaggtagg
    15841 gttggccgcg tggccaggat cgaccgtccg ccacccggcg aggagttgaa caccggagcg
    15901 accacgcagg cgcgcctcgc cgcgaccgcg atgctcgtcc gcgacggcac cgagggactc
    15961 gaggtgctcc tcgtgcagcg caaccccgag cagcgcttca tgggcggagc gtgggtcttc
    16021 ccgggcggcg ccgtgcacga cgacgacgcc gacccggagt cggcggcggt ccgcgagctc
    16081 gaggaggagg ccgggctgcg catcgaggac gttcgcgagc tcgtgccgtt ctcgcgctgg
    16141 atcacgccgg tcgaggtgaa gatccgcttc gacacgttct tctacctggc ggccgcgccg
    16201 ccggacgccg aggcgcgcgt ggacggcgcc gagtgcgtgg acgcgcgctg gatccgcccg
    16261 gccgacgcgc tcgacgcggg tacgcgcggc gagctcatgc tggtattccc cacgatcaag
    16321 cacctcgagc agctcgccga gctcgaatcg gtcgagcacg ccctcgagac ggcgcgctcc
    16381 cgccaggtgg agccgatcct gccgaaggtc gtgtacggcg agggcgaggc ccatgtcctg
    16441 cttcccggcg agccggggta cccggactga cctcggtccg caacctcgcc gcgcgtgtgg
    16501 ggttagcact ggcgtgaggc gcctcctgac catggctctc gcgcttgccg cggtgctggc
    16561 cctgatgggc ggcacgctgg cgatcgccga cacgctgctc ggcgacgccg gcccgaacga
    16621 gctgaccggc acggccgagc ccgacggcct ctggggggag gagggcgacg acaagctcac
    16681 cggcctggcc gccaacgatt acctggaggc cggtccgggc agcgacaagg tcgacgcggg
    16741 ccccggcgac gacttcgcca ccggcggcag cggtgaggac gagctgaaga tgggcagcgg
    16801 tcgcgacgtc ggctacgccg gtcccggcaa cgactcggtc ctcggcgagg acggcgacga
    16861 cgtgctgctc ggccagctcg gcgacgaccg cgtgttcggc ggacccggga acgaccgcct
    16921 ctacggcggc acgggcaacg acttcgtcga cggcggcacg ggcaacgacc agctcttcgc
    16981 cgacgtgggc atcaacacga tgctgggcgg cgccggcaac gaccgcttca tggtccaggg
    17041 tggcgccacc tcgaacatcg agggcggctc gggcgacgac aaggtctccc tctcgagcgg
    17101 cgacgacaag gtcgacggcg gcggtggcga cgaccggatc tcgaccggct ccggcgacga
    17161 caccgtggac acggtggacg gccgccgcga cgtggtcaag tgcggcaagg ggaccgacac
    17221 cgtgcgcgcc gacaagatcg acaggctgac cgactgcgag aacgtcaccc gcgcgagccc
    17281 caagggcacg cggcggcgct agacctcccc caccctgtat aagacgccca gtggcgcagc
    17341 agggtctgac agtcgcgatc accggcccga ccggcgacct cggcatcgcc atggtcgagg
    17401 cgctcgagcg ctcgcgtcgg gtcaagcgca tcgtcggaat ggcgcggcgc tcgttcgacc
    17461 ccaaggaccg cggctggagg aagaccgagt accgccaggg cgacgtgcag gacgccgcga
    17521 gcgtgaagga cgcggtgaag ggcgccgacg tggtggtgca cctcgcgttc gcgatcctca
    17581 ccgcgggcaa cgcgagccgc ggcatcaacg tggacggctc gaagaacgtg ttcgaggccg
    17641 ccgtggccgc cggggccgaa cgcatctgtt acgcgtcgag cgtcgcggcc tacgggttcc
    17701 acgatgacaa cccggagtgg ctcaccgagg acatcccgcc gcgcggcacg cccgagcacc
    17761 cgtactcggc ccagaaggcg gaggtcgagc agatcctcaa cgccgcgctg gcgcgctcga
    17821 gtcgcacgtc ggcgtgggtg ttccggccct gcatcgtcgc cggcccgaag gcgaccacct
    17881 tcctcgagga gatcccctac ttccggctct ccgagcgcat gcccgacccg gtccggcggc
    17941 tcgtcggcgg gatgccgatg ctgaagccgg tgatccccga caccgggccg ccgttccagc
    18001 tcgtgcacga ggacgacgtg gcgagcgcgt tcgtggccgg cgtgcacggc cgcggctcgc
    18061 cggggccgta caacctcgcc gggcgcggca agctcacgct gagcgacgtg gccgacgcgc
    18121 tcggctggta ctcggtcccg ttcccggacc tcgcgctcga ggccacgatg gaggtgctgg
    18181 cccgcctgcc gcggctgccc gagcaggcgg cctggatcca gtcggcgagg cggccggtgc
    18241 tgatgcgcac cacccgcgct cagaaggagc tcggctggcg cccgaagcac accgcccgcg
    18301 caacgctcaa gcagatgatc gaggcatacc gcagcgagca ggcgggcgtc gaggccggcg
    18361 ccgcctaggg cgcctcagag cgccgcgcgg aacaggcgct cgtagtcctc ctcggtcggc
    18421 tcgcgcgggt tcatcagcgt cgtgccgtcg cccatcgcgc cctcgatcag ggccgggatg
    18481 ccgtcctccg gcacccccac ctcggacagc cgcgtgggca ggccgaggcc ggacacgagg
    18541 ctccccacgt gggtcgcgag cgcgtcgccg acggcctcgt cggacccgcc cgcctcgatc
    18601 gtgagcagct cggcgagatc ccggtagcgg tcggcgatct ccgcgccgcc cgccgcgttg
    18661 aagcggatca cgtgcggcag gttgatcgcg ttcgccacgc cgtgggccac gccgaagtgg
    18721 gcgccacacg cgtgcgacat cgcgtgcgtc gagccgaggt acacggagat cgcgagcgag
    18781 gccgccacca gcatgttgcc gcgcgcctcg tcgtcgccgg cgccgccgtg gaccgcgcgc
    18841 tcgaggttgt cgcggatcag ccgcagggcg tggagtgcct ccgcgtcgct gaacgggctc
    18901 gactcgaccg tcacgtaggc ctcgatcgcg tgggtcatcg catccatgcc ggtggccgcg
    18961 gcgatcgcgg gcggcagcgt gcgcgtgcac tcggggtcga gcacggcgag ccgcggaaac
    19021 agcggcacgt cggccagctc gagcttcacc ttggcctcgt ggtccttgac caccacggcc
    19081 atcgacacct ccgagcccgt ccccgcggtc gtcgggacgc acgccaccgg ggcaaccggg
    19141 agcgcctggc ccattccgtc gttcgcgcgc ggcagcgcgt agaggccctc gtagtcgtgc
    19201 accgtcccgc cgtgggtgaa gagcacggcc gccgccttgg cggtgtccat caccgagccg
    19261 ccgccgacag cgaggaacga gtccgccccc gcggccctgg cctcctccgc gcagcgcgcc
    19321 acgacctcca cgcctgagtc ctgcggcacg tcgtcgaagg agccggccag ctccagcccg
    19381 ccgtccgtga cgcccgcctc gacgcgatcg accaggcccg tgccgcggat cacctgatcg
    19441 gtcaccacga acacacgctc ggcgccctcc ttcatcaact cgaacccgac gccctcgagg
    19501 agcccgtgcc ccgcgacgac ccgggtccgc acgatgtgct gcgcgaagtc cttgaacgcc
    19561 gtcacgccgc gtccgccagg tcctggcgcc ccgcgtccac gagctgctgg cggtagcgcg
    19621 ggaagaccgg cttgaccagt ggcacgagct tgaggacctt cgcgaccgga cccttggcct
    19681 tcatctgccc gcgcgccagc gccacggtca cgttgacctt gccgagccag aagcggtgag
    19741 cggtgtcggc gtccatcgtc atgaccacgt ccgggtcgag gtcgctctcg ccgaggtcga
    19801 cctgcggctc gacgtcctcg cgcagccgca ccgtgatctg cgactcgggg ttgcggtact
    19861 ggtactggac cgtcgcgttc gccttgcgga acttcgccgc cagctcgtca tcggtcacga
    19921 ggtcctggaa cagcttgccg atgtgcgcgt acacctcgtc ggcgtccttg aagtaggcca
    19981 cttgcttccc ctctccccgg ttgagcggcg attgtcgcct atcgggcgac gaggcggtag
    20041 acgccgccgc tcagcgacgt cacgtagatc cggccgagcg cgtcctcgcc gaacgacgag
    20101 agcccgctta ggcgcaggcc ggtcgagcgg ttgcccgagg cccccttcgt cgagatgcgc
    20161 atcgagtaga tcgtcgggtt gcagttgtcg ccgtgcaggt agcgcccggc gagtgccctc
    20221 agcgcggggt cgcggaccac gtagccgccg atcgtcgcgc agttgccgtt ctcgcgcagg
    20281 cgcttcacga gcacgggctt cacgtggccg ctcacggagc cctcgcggta gcgggagctc
    20341 gcctcgaaca cgctccagcc gaagttcgcc gcccgcccgc gcttcggggg gtagtcgatc
    20401 tcgtcgaact cgttctgccc cacgtcgccg atcgcaagcc cgccgccctt gcggtcgaac
    20461 gagaagcggt aggggttgcg cagcccgcgg gcccagatct cgccgcgcgc gccgctgcgg
    20521 tcgcggtacg ggttgtccgc cggtatgtcg tagccgccgc ccgggcgggg gtcgatccgc
    20581 aggagcttgc cgagcggcga gccgaggtcc tgcgcgctgc gcagcgggtc gccgccgcca
    20641 ccgccgtcgc ccgtggccag gtagagcatc ccgtcgggac cgaactggag ctggccgccg
    20701 ttgtggttgc cgaactggct gtgctcgatc cggatgacct cgcggccgct ccccgggtcg
    20761 gcgagctgcg cgttggacga gcgccggaac tggagcacgc ggatgtcgcc gtcgtcgccc
    20821 gtgacgtaca cgtagaagcg accggacttc gcgtagtccg gcgcgaaggc catcgacagg
    20881 aggccgcgct cgccgcagca gctcacctcg ccgcggatgt cgaggaaggc gcggctgcgc
    20941 agcttgtggt cgaccagcac gcggatccgg ccgggctgct cggtgacgaa cacacggcgc
    21001 ttgtcgcccg gcggggcggt cacgtaggtc ggctggtcga aggtgccgat ccgggcgagg
    21061 cgcagccgcg aggacgccgc cgcacgcgca ggcgcctcgg cgacggccgc caccggctcg
    21121 gcggacagcg tctcctcgcg ctcgtcgccg ttgcaggcgg cgagcgtggc gatggccgcg
    21181 atcagggcga gcagtaggag tgcgcgtcgc atcacggcgg gaacgatggc agcggagtgt
    21241 tgatctctcg taaacgcgcg gccgtacaac ctgtatgaag gctctcgtta caggtgcgac
    21301 cggcttcgtc ggcgggcggc tggtggaggc cctcgccgag cgcggggccg acgtgcgctg
    21361 cctggtccgc gacgccgagc gcgctcagca cctggccgac gccggccacg agctgcacga
    21421 gggcgacgtg ctcaagcccg agacgctcgc gggcgccggc gagtacgtcg acgtcgccta
    21481 ctacctcgtc cactcgatgg gccgcggctc cggcgacggc ttcgaggagc gcgagcgccg
    21541 cgccgcgcgc tcattcgcgg agatggcctc ccgcgaggga gtcgagcgcg tgatctacct
    21601 cggcggcctc ggcgacgagc cggagtcgga gcatctgcgc agccgtcacc agaccgccgc
    21661 gatcctctcc gagcacgggc cgccgctcac ctacttccgc gccgcgatgg tcgtcggc
//
LOCUS       DASVPL010000166        10060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_333108, whole genome shotgun sequence.
ACCESSION   DASVPL010000166 DASVPL010000000
VERSION     DASVPL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333108"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VF517_08595"
     CDS             complement(<1..432)
                     /locus_tag="VF517_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8053039.1"
                     /translation="MALQRMDNVLIVVEDLEAAKAFFTELGMELEAEQQVEGPWVGSV
                     VGLDDVRADIAMMRTPDGHGRVELSRFHTPPAVRAEPEDAPSNALGLRRIMFAVEGID
                     DVVARLRGHGAELVGEIAQYEDVYRLCFVRGPEGIIIGLAEQ"
     gene            719..1174
                     /locus_tag="VF517_08600"
     CDS             719..1174
                     /locus_tag="VF517_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053040.1"
                     /translation="MKMTHDAYAGYLLDFNQPAFGGDDLGHMLLGAQPRPLDVNGTVG
                     QTWPRRSTGAPDTELRLPARTDGPRARRLAIVRVVRVGDADALVLRSPPMPAGGIHSG
                     HVIVLWNLAGRGYEVSLHLADSRRPGRYTERERIRAAVTVARSYRPQSG"
     gene            complement(1162..1566)
                     /locus_tag="VF517_08605"
     CDS             complement(1162..1566)
                     /locus_tag="VF517_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8053041.1"
                     /translation="MDWTLEVVVVPVSDVDRAKEFYADVVGFKLDHDVRPTEALRVVQ
                     LTPPGSGCSIVIGEGLGAMEPGSLDGVQLVVSDIDAARAELVERGLDVSEIKQFEPVD
                     RDGSRFVFFKDLDGNGWAVQEIRARGGAPQPD"
     gene            1677..2012
                     /locus_tag="VF517_08610"
     CDS             1677..2012
                     /locus_tag="VF517_08610"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8053042.1"
                     /translation="MHALDVLGDPVRRRILELLADGEQPAGTVTAEIQRDFGISQPAV
                     SHQLRVLRDNGFASVRAEGTRRLYSVHPAPLQSVDEWLAPFRKFWNQRLDALGTELAR
                     GKRKRAKAP"
     gene            2009..2590
                     /locus_tag="VF517_08615"
     CDS             2009..2590
                     /locus_tag="VF517_08615"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8053043.1"
                     /translation="MTTRPRTATEPEPQLRHRRIDAGDARVAVFTRTYDAAVDDVWDA
                     CTNPDRLRRWYVPVSGDLRLGGTFAQPPMGTGTIVACEPPRYLKLSLGGGADEIELRL
                     APADEERTVLELQHATTLDEHEIGGQMYDAIFCMGGGYYPRLAALGRHLDGTLPDDYD
                     TLTFHERPENRPIIERGSAAMAALLAAEPKRDG"
     gene            2931..3557
                     /locus_tag="VF517_08620"
     CDS             2931..3557
                     /locus_tag="VF517_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8053044.1"
                     /translation="MVELAVVGGSALYRESIRRVLDDAGGLEVVGTAASLAEAAPLVE
                     RAHPDVLVVDLQWERDLAGLASIKERHPDLRVVVLGVDGDEATVVGCAAAGVMGYLAR
                     ETTAADLVKAIRQVAKGELACPPAIVETLVRGLSRRPGRTATAALDGLTRRERDVIDL
                     LGLGLTNKQIAERLCIQVATVKSHVHSILTKLGLARRAEVVALMRDRA"
     gene            3842..4846
                     /locus_tag="VF517_08625"
     CDS             3842..4846
                     /locus_tag="VF517_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8053045.1"
                     /translation="MAAEPRPSVLVVGGSRIVPSRTRALLEAVATAVEQAGAVPELWD
                     VRQPAQPGALRGAASRAQALVVVTPTYHASYSGLVKHALDQLDPGIVGGKPVALMATT
                     GPVPTPQALDHLRIVAGALDAFAIPAQIVATEAGFQQRDGRFQITDELLHQRVRTVVQ
                     ELVLVAWRLDAPGEPRLHPEPARNGRATAAAATVDLLRRRPGDLPDQIKRAVEYIRAN
                     FAKGPLPLDDVAREAHMSRYHFSRTFKRVTGVRFIEYVTRLRLARACALLAESEQTIT
                     AVALAVGYQDLRPFERTFKKEIGYTPSQYRRRVRAGLEDPPPTGLGPDTAGGAPPGTG
                     "
     gene            complement(4863..6338)
                     /locus_tag="VF517_08630"
     CDS             complement(4863..6338)
                     /locus_tag="VF517_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEX8053046.1"
                     /translation="MTRRGVVAGAAAGAVVAAVPGRAAARTRRKPATPRAEVVVVGAG
                     LSGLAAAEAIRRAGRSVIVLEARDRVGGRVLNEPIGDGEVIEVGGQWVGPTQDVVRDW
                     ARELGVETFATYNEGDYLFLREGRKLPYRPDTPLLGAIPPDPGSAEAGAALAQLNEMA
                     AAVPLDAPWQAPRAEEYDGKTADTWIRENTASPGGRFLVDLGVGALYAAEPRDVSLLH
                     LLFYIRAAGDEQNPGNFERLLNTAGGAQESRFLGGSQEIALRAAARLGERVRLGWPVR
                     RIGQDRKQAVLRGPRGNVAAKRVVVTLPPALAGRIDYRPAMPALRNQLTQRMPQGSAI
                     KCQAIYDRPFWRERGLSGQAVSDQGPARITFDNSPPDGGPGVLLGFVEGSFARRHSRL
                     SEGERRAAVLRNFADLFGEEALAPRRYVERDWSSDPWTRGCFVGFTPPGVLLDYGEAL
                     RRPVGRIHWAGAETATFWNGYMDGAVRSGRRAAAEVLAALG"
     gene            complement(6374..6799)
                     /locus_tag="VF517_08635"
     CDS             complement(6374..6799)
                     /locus_tag="VF517_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053047.1"
                     /translation="MTLGDDTTAVLAAAGLMFIWALSLGIVKYRQMATSENALAHPYT
                     DTAHRAALLYSFAILLVATFVELSAWSTLVNLLAAGALVLFFAAAVFGYMVHGLRRDT
                     DNQFRDPVPGTHAFMYALILAEIGGFSVLLAGFADAQIF"
     gene            complement(6871..8949)
                     /locus_tag="VF517_08640"
     CDS             complement(6871..8949)
                     /locus_tag="VF517_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053048.1"
                     /translation="MRQLGRQLIQHHEGKGHPRMIRLRLAAACTFFAALATAPAALAA
                     PGSLDPTFGSGGIAATVLGSDDYVRDIALQPDGKTVAAGTAWDGTRYRFALARYDVDG
                     SLDPAFGTAGKVLTGIGSSSVAQAVALQPDGKIVAAGTGSGDFAVARYNADGSLDSSF
                     SADGMATAAVGNEATAWDLAIQPDGRIVLAGQAKVSGAQRFALARFTGAGDLDTSFGT
                     RGKTTTPLGTDGAVAMALVRQADGRLVAAGSASESGGARFALARYSAGGSLDTSFGSG
                     GSVLTTVGGGGQATGLIADPAGRLVAVGGAVDGFVYKAALTRYSADGALDSSFGTRGV
                     AFAHAGQMATIASGVARQQDGRLVVVGESIGASGSTFAVSRFEADGALDSTFGSGGSV
                     TTAIGQRSGAYDVAVQADGQILAAGAGNGNADGSARQFALARYTAAGDEGTGGSGGST
                     GGTGDGGSGGSTGDGSTGTGDGSTGTGDGSTGTGDGSTGTGDGSTGTGDGGSGGSTGD
                     TGGSTGTGDGSTGTGDGSTGSTGDTTGSTGSTGGGVPIPTPEPGGGLAGQIVPGGLEL
                     IPANVRPIVLPPQPLKLQNTGIVPVRVRCIEIPAGSCRGTILITVPEQVVLPYVRHRR
                     VGPAASRRVTVGRASVRIPSGGTATARPRLTGPAARALRRRGSLRVVVTATMTVNGTA
                     RISTRTLTLR"
     regulatory      complement(8928..9007)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            9129..>10060
                     /locus_tag="VF517_08645"
     CDS             9129..>10060
                     /locus_tag="VF517_08645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8053049.1"
                     /translation="MIGRQLGGYRIESVIGRGGMGIVYLAEQTRLERRVALKVITPEL
                     AHDVGFRTRFERESRLAASIDHPNVVPVYEAGEADGLLYIAMRHVRGTDLKALLTQGP
                     LDPGRAVELVGQVAAALDAAHASGLVHRDVKPGNILIEPIPGGERAYLSDFGLTKRLS
                     SASGITETGFVVGTLDYIAPEQVQGAAIDARTDVYALACVLFHALSGRVPFVRENDMA
                     KMYAHANLPAPALLEAAPHVPAALGEVVARGMAKEAGERFASAGDLGRAASAAVHGHA
                     PTMAERSVAVGAAAPGQETVADTPLPPPPDPPTA"
BASE COUNT         1375 a         3688 c         3662 g         1335 t
ORIGIN      
        1 ctgctcggcc agtccgatga tgatgccctc ggggccgcgc acgaagcaga gccggtagac
       61 gtcctcgtac tgcgcgatct cgcccacgag ctcggcaccg tggccgcgca ggcgggcaac
      121 gacgtcgtcg atgccctcga ccgcgaacat gatccggcgc aggccgaggg cgttggacgg
      181 cgcgtcctcc ggctcggccc tgaccgccgg cggcgtgtgg aaccgcgaca gctcgacccg
      241 gccgtggccg tcgggggtgc gcatcatcgc gatgtcggca cggacgtcgt cgagcccgac
      301 gacgctgccc acccaagggc cctcgacctg ctgctcggcc tccagctcca tgcccagctc
      361 ggtgaagaac gccttggcgg cctcgaggtc ctcgacgacg atgaggacgt tgtccatgcg
      421 ctgaagcgcc atccggcgga gaataggtcg ggtgcttcga ggacacgcac acgggcccga
      481 cttgcggctt cgaatcgcga tcctcgccgc tgcagtcatc gcggggtgcg gcagctccaa
      541 cggcgagcgc gcgcgggagc gatcgcagac cgacccagcc tcgccccggc ctggcgtcat
      601 accgctggct cgcaccccgg cggtggtgag cgacgcctgc cggagtgcgg ccgcgtccgc
      661 tcggctcgcc tgccccgggc tgttcccagc gcgaggcgat gtgacgaagg tccgagccat
      721 gaagatgacc cacgacgcgt acgcgggcta tctgctcgac ttcaaccagc ccgcgtttgg
      781 tggcgacgac ctcggccaca tgctgctcgg agctcagcca cggccgctcg atgtgaacgg
      841 cacagtgggt cagacgtggc cgcggcggag cacgggcgca cccgacaccg agctgcggct
      901 tccagcgcgc accgatggcc cgcgcgcccg gcgtttggcg atcgtccggg tcgtccgggt
      961 cggggacgcc gacgccctcg tcctcagatc gccgccgatg cccgccgggg gcatccactc
     1021 cggtcacgtg atcgtcctgt ggaacctggc cggacgcggg tatgaggttt cgcttcacct
     1081 cgccgacagc cgcaggccgg gccgttacac cgagcgagaa cggattcggg cggcggtgac
     1141 ggtcgcacgg agctaccgcc ctcaatcagg ctgaggtgcg ccgccccgcg cgcggatctc
     1201 ctgcacggcc cagccgttcc cgtccaggtc cttgaagaac acgaagcgcg agccgtcccg
     1261 gtcgaccggt tcgaactgct tgatctcgct cacgtcgagc ccccgctcca cgagctccgc
     1321 gcgcgcggcg tcgatgtcgg acacgaccag ctggacgccg tcgaggcttc cgggctccat
     1381 cgctcccaac ccctcaccga tgacgatcga gcagccggag cccggcggcg tcagctgcac
     1441 cactcgcaac gcctcggttg gccgcacgtc gtggtccaac ttgaagccga ccacatccgc
     1501 gtagaactcc ttcgcccggt ccacatccga gacgggaacc acgaccacct cgagcgtcca
     1561 atccaagatc gatctctcct ctgtgtgctt gggaacggcg agtgcatcgc ctggcccgag
     1621 agtatcagtt tatatttata tatagatccc cttatctccc cgctacgctt ccccgcgtgc
     1681 acgccctcga cgtcctcggc gatcccgtcc gacgccggat cctcgagctt ctcgccgacg
     1741 gcgagcagcc cgccggcacg gtgaccgcgg agatccagcg ggacttcggg atctctcagc
     1801 ccgcggtctc gcaccagctg cgcgtcctgc gcgacaacgg gttcgcgtcc gtgcgcgcgg
     1861 agggcacgcg ccgcctgtac tcggtccacc ccgccccgct ccaatcggtc gacgagtggc
     1921 tcgctccgtt caggaagttc tggaaccagc gcctcgacgc gctcggcacg gagctggccc
     1981 gcggcaagcg gaagagggca aaggcgccgt gacgacccgc ccgcgcaccg ccaccgagcc
     2041 ggagccgcag ctccgccatc gccgcatcga cgccggcgac gcacgggtcg cggtcttcac
     2101 tcgcacctac gacgcggccg tggacgacgt gtgggacgcc tgcacgaacc cggatcggct
     2161 acggcgctgg tacgtcccgg tcagcggcga cctgcgcctc ggcggcacct tcgctcagcc
     2221 gccgatgggt accggcacga tcgtcgcgtg cgagccaccc cggtacctga agctgtccct
     2281 gggcggcgga gccgacgaga tcgagctgcg tctcgcgccc gcggacgagg agcgcacggt
     2341 gctggagctc cagcacgcga cgacgctcga cgagcacgag atcggcggtc agatgtacga
     2401 cgcgatcttc tgcatgggcg gcggctacta cccgcgcctc gccgccctcg gccggcacct
     2461 ggacggaacg ctgccggacg actacgacac cctcaccttc cacgagagac cagagaaccg
     2521 ccccatcatc gagcgcggca gcgccgccat ggcggctctg ctagcggccg agccgaagcg
     2581 cgacggctag ccccgcgcgg gcgatcaggg aaccagcggc cagggccacg gccgggcgta
     2641 caggcggttc ggcggtgtcg acttcatcag gacctccttg cctccatcgg cacggccggc
     2701 gtcccgcgtc cggcgcgcgt tgcgcaatgg acgaccccgg ttgcgccaag ccgggatcgc
     2761 ttgcgccagc ccggcacctg ttgcccgacg cgggggcgcc acctgctagt tcgatcggga
     2821 cgcgccgagg agctgggctg cggtcggcag tagatcgtcg gggttgatcc cccgtaagcg
     2881 gcgtcggtgt gcgaacatat gttcgtatgg gacgacaccc gggacgccga atggtggagc
     2941 tagccgtcgt cggtggtagc gcgctgtacc gggagtcgat acggcgcgtg ctggacgacg
     3001 cgggcggcct cgaggtcgtg ggcacggcgg cctcgctcgc ggaggcggct cccctggtcg
     3061 agcgggccca ccccgacgta ctggtggtcg acctccagtg ggagcgcgac ctcgccggac
     3121 tggcgtcgat caaggagcgg cacccggacc tgcgcgtggt ggtcctgggc gtggacggcg
     3181 acgaggcaac ggtcgtcggc tgcgccgcgg ccggcgtgat gggctacctg gcccgcgaga
     3241 cgaccgcggc cgacctcgtc aaggcgatca ggcaggtggc caagggcgag ctggcgtgcc
     3301 cgccggcgat cgtcgagacg ctcgtgcgcg gactgtcgcg acgaccaggg cgcacggcga
     3361 cggcggcgct cgacggcctc acgcggcgcg agcgcgacgt gatcgatctg ctcgggctcg
     3421 gcctcacgaa caagcagatc gcggagcggc tctgcatcca ggtagcgacc gtcaagagtc
     3481 acgtccacag catcctcaca aagctcgggc tcgctcgccg cgctgaggtc gtggcgctca
     3541 tgcgcgaccg cgcgtagggg tatctggacc cgcgacggcc gcggtctgcg ccctgcggtc
     3601 tagatctccg cagcgcaaga tcgatccttg gaccgatgac aacggacgcc gcacgcgaga
     3661 gggttgctgt tcagcgtggg cgtacctggg agacacggca ccgagcacac gggccgggtc
     3721 ccggacggac gcgcttctcg aggccggact cgtgcgccgg ccgcgggcca gacgagcggg
     3781 agtggggctc gacatggccg cggccggtgc gcgccgtcct cacccgcggg cctccgccgg
     3841 aatggccgcc gaaccccggc ctagcgtcct ggtcgtcggc ggcagcagga tcgtgccgtc
     3901 gcgaacccgc gcgctgctcg aggccgtggc gacagccgtg gagcaggcgg gcgcggtgcc
     3961 cgagctctgg gacgtacgac agccggcgca acccggcgcg ctgcggggcg ccgcctcgcg
     4021 cgcccaggcg ctcgtggtcg tgacgcccac ctaccacgcc tcctactccg ggctcgtaaa
     4081 gcacgcgctc gaccagctcg atcccgggat cgtcggcggc aagccggtcg ccctcatggc
     4141 caccaccggc ccggtcccga cgccgcaggc gctcgaccac ctccggatcg tggccggcgc
     4201 gctcgacgcg ttcgccatcc ccgcccagat cgtggccacc gaggccggct tccagcagcg
     4261 cgacggccgc tttcagatca ccgacgagct gctgcatcag cgcgttcgta ccgtcgtgca
     4321 ggagctggtg ctcgtcgcgt ggcggctcga cgcgcccggc gagccccgcc tgcacccgga
     4381 gcccgcgcgc aacggccggg cgactgcggc ggccgcaact gtggacctgc tgcgccggcg
     4441 gcccggcgac ctgcccgacc agatcaagcg ggcggtcgag tacatccgcg cgaacttcgc
     4501 caagggcccg cttccgctcg acgatgtcgc gcgcgaggcg cacatgagcc gctaccactt
     4561 ctcgcgcacg ttcaagcggg tcacgggcgt ccgcttcatc gagtacgtga cgaggttgcg
     4621 gctggcgcgg gcctgcgcgc tgctcgcgga gagcgagcag acgatcacgg cggtcgcgct
     4681 cgcggtcggc taccaggacc tgcgcccgtt cgagcggacg ttcaagaagg agatcggcta
     4741 cacgccgtcg cagtaccgca ggcgcgtgcg cgccggcctg gaggacccgc cgccgaccgg
     4801 gctcggtccc gacaccgctg gcggtgcgcc gccgggaacg gggtgagggc cgccggccgt
     4861 cctcagccca gcgccgcgag cacctcggcc gccgcgcgcc gcccggaccg caccgcgccg
     4921 tccatgtagc cgttccagaa cgtcgccgtc tcggctcccg cccagtggat gcggccgacc
     4981 gggcgccgca gcgcctcgcc gtagtcgagc agcacgccgg gaggggtgaa gccgacgaag
     5041 cagccgcgcg tccaggggtc gctcgaccag tcgcgctcga cgtagcgccg cggcgcgagc
     5101 gcctcctcgc cgaacaggtc ggcgaagttc ctcagcaccg cggcgcggcg ctcgccctcg
     5161 gacaggcgcg agtggcggcg tgcgaacgat ccctcgacga agccgagcag gacgcccggg
     5221 ccgccgtccg gaggcgagtt gtcgaaggtg atccgcgccg gaccctggtc gctgaccgcc
     5281 tgcccggaca ggccgcgctc gcgccagaac gggcggtcgt agatcgcctg gcacttgatg
     5341 gctgagccct gcggcatccg ctgcgtgagc tggttgcgga gcgcaggcat cgcgggtcgg
     5401 tagtcgatcc gccccgccag ggctggcggc agggtgacca ccacgcgctt cgcggccacg
     5461 ttccctcggg ggccgcgcag cacggcctgc ttgcgatcct gaccgatccg ccgcaccggc
     5521 cacccgagcc gcacgcgctc gccgagccgg gcggcggcgc gcagcgcgat ctcctgcgag
     5581 ccgcccaaga agcggctctc ctgcgctccg ccggcggtgt tcagcagccg ctcgaagttg
     5641 ccggggttct gctcgtcccc cgctgcccgg atgtagaaga ggaggtggag cagcgagacg
     5701 tcgcgcggct cggcggcgta gagcgcgccc acgcccaggt cgacgaggaa gcgaccgccc
     5761 gggctcgccg tgttctccct gatccaggtg tcggcggtct tgccgtcgta ctcctcggcc
     5821 cgcggcgcct gccatggcgc gtcgagcggc accgcggccg ccatctcgtt cagctgcgcc
     5881 agcgccgcgc ccgcctcggc ggagccggga tcgggcggga tcgctccgag cagcggcgtg
     5941 tcggggcggt acgggagctt gcggccctcg cggagaaaga ggtagtcgcc ctcgttgtag
     6001 gtcgcgaacg tctcgacccc gagctcccgc gcccagtcgc ggaccacgtc ctgcgtcggg
     6061 ccgacccact gcccgccgac ctcgatcacc tcgccgtcgc cgatcggctc gttcagtaca
     6121 cggcccccca cccggtcgcg cgcctcgagc acgatcaccg agcggccggc acggcggatg
     6181 gcctcggccg cggcgagacc gctgaggccg gcaccgacca cgaccacctc ggcgcgcggc
     6241 gtggccggct tgcggcgcgt gcgggcggcc gcacgtcccg gcaccgccgc cacgaccgct
     6301 ccggccgccg ccccggcgac gacaccgcga cgcgtcacgc cgcggcggtc tcgctcgctc
     6361 gacggaggca cgcctagaag atctgcgcgt ccgcgaagcc cgcgagcagc acggagaacc
     6421 cgccgatctc ggcgaggatc agcgcataca tgaaggcgtg ggtgcccggc accgggtcgc
     6481 ggaactggtt gtcggtgtcg cggcgcaggc cgtggaccat gtagccgaac accgcggcgg
     6541 cgaagaagag gacgagcgcg cccgcggcga ggaggttcac cagggtgctc caggcgctca
     6601 gctcgacgaa cgtcgcgacg agcaggatcg cgaacgagta gaggagcgcg gcgcggtggg
     6661 ccgtgtcggt gtaggggtgc gcgagcgcgt tctcggaggt cgccatctgg cggtacttca
     6721 cgatcccgag cgagagcgcc cagatgaaca tcaggccggc ggccgccagc accgccgtcg
     6781 tgtcgtcccc cagcgtcacg gcgagggatt aaaccggagc ggtggcggcg cgtgtgtgac
     6841 aacacgccgc cgcggggacc ggggacccgg ctaccggagc gtgagggttc gcgtcgagat
     6901 ccgtgccgtt ccgttcacgg tcatcgtggc ggtcaccacg acgcggaggc tcccccgcct
     6961 gcgcagggcg cgggccgcgg ggccggtcag cctgggtcgc gccgtcgcgg tcccgccgga
     7021 cgggatcctg acggaggcgc gaccgaccgt gaccctgcga ctcgccgcgg gcccgacgcg
     7081 ccggtggcgc acgtagggga ggacgacctg ctcggggacc gtgatcagga tcgtgcctct
     7141 gcacgatccc gccgggatct cgatgcagcg gacccggacc ggcacgatgc cggtgttctg
     7201 gagcttcagc ggctggggcg gcagcacgat cggcctcacg ttcgcgggga tcagctcgag
     7261 gccgcccggc acgatctggc cggcgaggcc gccgccgggc tcgggggtcg ggatggggac
     7321 gccgccgccg gtggagccgg tgctgccggt ggtgtccccg gtgctgccgg tggatccgtc
     7381 gccggtgccg gtcgagccgt cgccagtgcc ggtggagccg ccggtgtcac cggtcgaacc
     7441 gccgctgccg ccgtcgccgg tgccagtgga tccgtcgccg gtgccagtgg atccgtcgcc
     7501 ggtgccagtg gagccgtcgc cagtgccggt ggagccgtcg ccggtgccag tggatccgtc
     7561 gccggtcgag ccgccgctgc cgccgtcgcc ggtgccgccc gtggagccgc cgctgccgcc
     7621 ggtgccctcg tcacccgcgg ccgtgtagcg cgccagcgcg aactgccgcg cggagccgtc
     7681 ggcgttgccg ttgccggcgc ctgccgcgag gatctgcccg tcggcctgca ccgccacgtc
     7741 gtacgcgccg ctgcgctgcc cgatcgcggt cgtgaccgag ccgcccgagc cgaaggtcga
     7801 atcgagcgcg ccgtccgcct cgaagcggga caccgcgaag gtggatccgc tggcgccgat
     7861 cgactcgccc actacgacga ggcgaccgtc ctgctggcgt gcgaccccgc tcgcgatggt
     7921 ggccatctga cccgcgtgcg cgaacgcgac cccgcgggtc ccgaaggagc tgtcgagcgc
     7981 gccgtcggcg ctgtaacggg tgagcgcagc cttgtagacg aaaccgtcga ccgctccgcc
     8041 gacggccacg agccgtccgg cgggatcggc gatgaggccg gtggcctggc ccccgccgcc
     8101 caccgtggtc agcaccgaac cgcccgatcc gaagctcgtg tcgagcgacc cgccggcgct
     8161 gtagcgggcc agggcgaacc gggctccgcc gctctcgctg gcgctaccgg ccgcgaccag
     8221 ccgcccgtcc gcctgccgca cgagcgccat ggccaccgca ccgtcggtcc cgagcggcgt
     8281 ggtcgtcttg ccgcgcgtgc cgaagctcgt gtcgaggtcg ccagcgccgg tgaagcgcgc
     8341 gagggcgaag cgctgtgcgc cgctgacctt cgcctgcccg gcgagcacga tccggccgtc
     8401 cggctggatg gcgaggtccc atgcggtggc ctcgttgccg accgcggcgg tggccatgcc
     8461 gtctgcgctg aacgaggagt cgagcgagcc gtcggcgttg tagcgcgcga cggcgaagtc
     8521 gcccgagccg gtgccggcgg cgacgatctt gccgtcgggc tggagcgcga cggcctgcgc
     8581 caccgagctc gagccgatgc cggtgaggac cttgcccgcc gtcccgaacg ccgggtcgag
     8641 tgagccgtcg acgtcgtagc gcgccagcgc gaagcggtag cgggtgccgt cccaggccgt
     8701 accggccgcg acggtcttgc cgtccggctg gagcgcgatg tcgcgcacgt agtcgtcgga
     8761 acccagcacg gtggccgcga tgccgccgct gccgaacgtc ggatcgagcg atccgggtgc
     8821 ggcgagagcc gccggcgcgg tggccagagc ggcgaagaag gtgcatgcgg ctgcgagcct
     8881 gagcctgatc atccgggggt gtcctttccc ctcgtggtgc tggatgagct ggcggccgag
     8941 ctgtctcaac gagctgagac ctctagcttt gcgtccccgc ctcgcgacgg gtttgccatg
     9001 agcagccgag tcgagggttg atcggccggt ggagccgcgg catgagcgtt ctggacggtt
     9061 gaccgaacgc cgggcccggc gctggcgcgg agccgcgccc caccggcgcg gcggctaagg
     9121 tcgcgcccgt gatcggcagg cagctcggcg gctaccggat cgagtccgtg atcgggcgcg
     9181 gcggcatggg gatcgtctac ctcgccgagc agacgcggct cgagcgccgc gtggcgctga
     9241 aggtcatcac gccggagctg gcgcacgacg tggggttccg gacgcggttc gagcgcgagt
     9301 cgcggctcgc ggcctcgatc gaccacccca acgtggtccc cgtgtacgag gcgggcgaag
     9361 ccgacgggct cctctacatc gccatgcgcc acgtgcgcgg gaccgacctc aaggcgctcc
     9421 tgacccaggg cccgctcgat ccggggcggg cggtcgagct ggtcggacag gtggctgccg
     9481 cgctcgatgc cgcccacgcg agcgggctcg tccaccgcga cgtgaagccg ggcaacatcc
     9541 tgatcgagcc gatcccggga ggcgagcgcg cctacctgtc cgacttcggg ctcacgaagc
     9601 ggctctcgtc ggcaagcggg atcaccgaga cgggcttcgt ggtcgggacg ctcgactaca
     9661 tcgcgcccga gcaggtgcag ggggccgcga tcgacgcgcg cacggacgtc tacgcgctcg
     9721 cgtgcgtcct gttccacgcg ctcagcgggc gggttccgtt cgtgcgcgag aacgacatgg
     9781 cgaagatgta cgcgcacgcg aacctgccgg cgcccgcgtt gctcgaggcg gcgccgcacg
     9841 tgccggccgc gctcggcgag gtggtggcgc gcgggatggc gaaggaggcc ggcgagcgct
     9901 ttgcgtcggc gggtgacctc gggcgggccg ccagcgccgc ggtgcacggc cacgcgccca
     9961 ccatggccga gcgcagcgtg gccgtgggtg ccgccgcgcc ggggcaggag accgtggcgg
    10021 acacgccgct cccgccgccc ccggacccgc ccacggcgca
//
LOCUS       DASVPL010000167         5185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_136596, whole genome shotgun sequence.
ACCESSION   DASVPL010000167 DASVPL010000000
VERSION     DASVPL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136596"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VF517_08650"
     CDS             complement(<1..249)
                     /locus_tag="VF517_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053050.1"
                     /translation="MTGEPPPDRSEEEREAARLARERARAERAERAERGGGARRGEPA
                     TARARALFRKPRAEPDGEGERSPLPEPEGAELELPRRPL"
     gene            complement(263..661)
                     /gene="ruvX"
                     /locus_tag="VF517_08655"
     CDS             complement(263..661)
                     /gene="ruvX"
                     /locus_tag="VF517_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX8053051.1"
                     /translation="MSRVLALDHGSARCGVAVSDPTGTLASPLPAVERPETERGLAAL
                     AALVRDTEAERVVIGLPRTLSGEEGVQAAAARSFAGRVARVVGVPVELHDERLTTRQA
                     QRSGGEADEDSRAAAHLLESWLASSPSQRP"
     gene            complement(690..3290)
                     /gene="alaS"
                     /locus_tag="VF517_08660"
     CDS             complement(690..3290)
                     /gene="alaS"
                     /locus_tag="VF517_08660"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX8053052.1"
                     /translation="MKAAEIRERYLAFFEEHGHRRMPSASLVPPTYDPSVLLTTAGMQ
                     PFKPYFRGDEQPPAQRLTSCQKCFRTTDIENVGLTARHLTFFEMLGNFSFGDYFKQGA
                     AEFAWELSTKGLGLDPERIWVTVFGGDEELGLGPDEEAIACWRAIGVPEERIVHLGRE
                     DNFWQAGPTGPCGPCSELYYDRGPGFGPDEDRPGDDTERILEYWNLVFMQYELHEDGS
                     LTPLPEQNIDTGLGLDRTAAILQDVPSVYENDQFAPLVELGRELAGVDEPSVRALRIL
                     ADHGRGMTFLLADGVVPSNEDRGYILRRIMRRAVQQGRSIGIEGAVLVPLVRRTIEVM
                     GDAYPALPESAAVIERWAAAEEEAFSHTLRQGERLLADLIARAKEEGTAWIAAEEAFK
                     LHDTFGFPYELTKELLGDEGLAVDDQGFEELMERAREVARGGAARSTNGHHDEVFDFT
                     RTSDFETEFVGYESLEAETVLGALKPLSGGRFLAKLRQSPFYAEGGGQVGDTGLVQAP
                     SANARVETVYRVGDDQLLELEVENGEPSEGERVRAVVDRDRRLATMSNHTATHLLHAA
                     LRKQLGEHVRQAGSYVGPDKLRFDFTHGERLTPRQAQSVEAEVNGWILANQPVRAVHT
                     TRTEAEAMGAMALFGEKYGDWVRMVEVEDVSRELCGGTHVAHTAEIGLFELVTETSSA
                     SNVRRIEAVTGPQGVGAALTRAREIRDLAGLLRVPEREVVEAVRKLTERVKELERKPR
                     ESDREAAGGLVESAEEVGGVRLVAQVVDAPDAKALLALSDQVRQTLGDSAVVLGTAVD
                     GRVHLVANVAPAVVERGVKAGEVIRAAAQVAGGGGGGRDTMAQAGGRDPEKLPEAIAA
                     ARLAIERVLG"
     gene            3559..4815
                     /locus_tag="VF517_08665"
     CDS             3559..4815
                     /locus_tag="VF517_08665"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX8053053.1"
                     /translation="MPLNRGTDPLVLAALVGLLALARRVRFEVGTLEACADQLAFIPL
                     LLLAPLPLVPVLVAAAYVLARVPDFLKGGMHPDRWLYFVGDAWHSIAAVAVVAALAPG
                     EISAEHLPVYGLAVGAQIAAGLLVIVVNDTLIHGFKPREIFGLSFSHSARIDALLSPL
                     ALITAAVAAQEPLAIAGVMPFYWLMAIFSAERRERYAAALELNQAYRGTVMVLSDVVD
                     AEDSYTASHCRSVVELAAAVADELGMGYEDRQELEIAALLHDVGKIAIPNEILNKPAK
                     LTDEEFALMKTHTIEGQALLDRVGGRLAKVGEVVRSCHERWDGRGYPDGLQGEEIPPA
                     ARVVFCCDAYSAMTTDRPYRQAMPQAEAIAELRKNSGTQFEPRVVEALVAVIEADLAG
                     ADDGRYTDALRAVLAGNSVPALELTA"
     gene            complement(4749..>5185)
                     /locus_tag="VF517_08670"
     CDS             complement(4749..>5185)
                     /locus_tag="VF517_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053054.1"
                     /translation="KRRYVGGILVMALGIALFAIAPVFLLALVGLGLTGVGNGLLLTH
                     ERLIFQKFVPDRLLGRAFALADTAGSWAFAIAFVGAGAALEQVGATALLLGASGGAAL
                     ICLWAWYALRTTWEESAPEPCYAVSSSAGTELPASTARSASV"
BASE COUNT          690 a         2029 c         1751 g          715 t
ORIGIN      
        1 cagcggacgc cgcggtagct ccagctcggc cccctcgggc tcagggagcg gcgagcgctc
       61 cccctcccca tcaggctccg cgcgcggctt ccggaacagc gcgcgcgctc gcgccgtcgc
      121 gggctcgccg cggcgtgccc cgccgccgcg ctcggctcgc tctgcacgtt ccgcacgcgc
      181 gcgctcgcgc gcgaggcgcg cggcttctcg ctcctcctcg gagcggtccg gtgggggctc
      241 gcctgtcaag ccgcgatcaa gcctacgggc gctgcgacgg cgacgacgcg agccagctct
      301 cgagcaggtg ggcggccgcg cgcgaatcct cgtcggcctc cccgccgctg cgctgcgcct
      361 gccgggtggt cagccgctcg tcgtgcagct ccaccggcac cccgaccacc cgcgccacac
      421 ggcccgcgaa cgagcgggcc gccgccgcct gcacaccctc ctcgcccgac agcgtgcgcg
      481 gcagcccgat cacgacgcgc tcggcctccg tatcccgcac gagcgccgcc agggcggcca
      541 gcccgcgctc ggtctcgggc cgctcgaccg ccgggagcgg gctcgcgagc gtgcccgtcg
      601 ggtccgacac cgcgacgccg cagcgagccg atccgtggtc gagcgcgagg acgcggctca
      661 tcgccgcgcg cgagcgcccg cgccgcgact catcccagca cgcgctcgat cgcgaggcgc
      721 gccgccgcga tcgcctccgg cagcttctcc gggtcgcggc cgccggcctg cgccatcgtg
      781 tcgcggccgc cgcctccccc gcccgccacc tgggccgccg cgcggatcac ctcacccgcc
      841 ttcactccgc gctcgaccac cgccggagcc acgttcgcga ccaggtgcac gcggccgtcc
      901 acggcggtgc cgagcaccac cgcgctgtcg ccgagcgtct ggcgcacttg gtccgagagc
      961 gcgagcagag ccttggcgtc cggcgcgtcg acgacctgcg ccacaagccg cacgccgccg
     1021 acctcctcgg cgctctcgac cagcccgccg gcggcctcgc ggtcggactc gcgcggcttg
     1081 cgctcgagct ccttgacgcg ctctgtcagc ttgcgcaccg cctcgaccac ctcgcgctcg
     1141 ggcacgcgca gcagccccgc gaggtcgcgg atctcccgcg cgcgcgtcag cgccgccccc
     1201 acgccctgcg gtccggtgac cgcctcgatc cggcgcacgt tcgacgcgct cgaggtctcg
     1261 gtgaccagct cgaacagccc gatctcagcg gtatgcgcca cgtgcgtgcc gccacacagc
     1321 tcgcgcgaca cgtcctcgac ctcgaccatc cgcacccagt cgccgtactt ctcgccgaac
     1381 agcgccatcg cccccatggc ctccgcctcc gtgcgggtgg tgtgcacggc gcgcaccggc
     1441 tggttggcga ggatccagcc gttcacctcc gcctcgaccg actgcgcctg gcgcggcgtg
     1501 aggcgctcgc cgtgggtgaa gtcgaagcgc agcttgtccg gcccgacgta cgagccggcc
     1561 tggcgcacgt gctcgccgag ctgcttgcgc agcgccgcgt gcagcaggtg cgtggcggtg
     1621 tgattggaca tggtcgcgag gcgccggtcg cgatcgacca ccgctcgcac ccgctccccc
     1681 tcgctcggct ctccgttctc gacctcgagc tcgaggagct ggtcgtcgcc cacccggtag
     1741 accgtctcca cgcgcgcgtt cgcgctgggg gcctggacca gcccggtgtc cccgacctgc
     1801 ccgccgccct cggcgtagaa cgggctctgc cgcagcttcg cgaggaagcg gcccccggag
     1861 agcggcttga gcgccccgag cacggtctcc gcctcgagcg actcgtagcc gacgaactcg
     1921 gtctcgaagt cggaggtccg ggtgaagtcg aagacctcgt cgtggtgacc gttcgtgctg
     1981 cgcgccgcgc cgccgcgagc cacctcgcgc gcgcgctcca tcagctcctc gaagccctgg
     2041 tcgtccaccg ccagtccctc gtcgccgagc agctccttgg tcagctcgta cgggaagccg
     2101 aacgtgtcgt ggagcttgaa cgcctcctcg gccgcgatcc aggcggtgcc ctcctccttc
     2161 gcgcgcgcga tcaggtcggc cagcaggcgc tcgccctggc gcagggtgtg cgagaacgcc
     2221 tcctcctcgg ccgccgccca gcgctcgatc acggccgcgg actcgggcag cgccggatag
     2281 gcgtcgccca tcacctcgat cgtccgcctg acgagcggca cgagcaccgc gccctcgatc
     2341 ccgatcgagc gcccctgctg caccgcgcgc cgcatgatcc gccgcaggat gtagccgcgg
     2401 tcctcgttgg acgggacgac gccgtcggcg agcaggaagg tcatgccgcg cccatggtcg
     2461 gcgaggatcc tgagcgcgcg gaccgacggc tcgtccacgc cggccagctc gcggcccagc
     2521 tccacgagcg gcgcgaactg gtcgttctcg tagaccgacg ggacgtcctg gaggatcgcc
     2581 gctgtgcggt cgagccccag cccggtgtcg atgttctgct cgggcagcgg cgtgagcgag
     2641 ccgtcttcgt gcagctcgta ctgcatgaag acgaggttcc agtactcgag gatgcgctcg
     2701 gtgtcgtcgc cggggcggtc ctcgtccggg ccgaagccgg gaccgcggtc gtagtagagc
     2761 tccgagcacg gcccgcacgg gcccgtcggc cccgcctgcc agaagttgtc ctcgcggccg
     2821 aggtgcacga tccgctcctc cggcaccccg atcgcccgcc agcacgcgat cgcctcctcg
     2881 tcgggcccga ggccgagctc ctcgtcaccg ccgaacaccg tcacccagat ccgctcgggg
     2941 tcgagcccga ggcccttcgt ggacagctcc caggcgaatt cggcggcgcc ctgcttgaag
     3001 tagtcgccaa aggagaagtt gccgagcatc tcgaagaacg tcaggtgacg agccgtaagc
     3061 ccgacgttct cgatatcggt ggtcctgaag cacttctggc acgacgtgag ccgctgcgcg
     3121 ggcggctgct cgtcgcctcg gaagtacggc ttgaacggct gcattccggc cgtggtgagc
     3181 agcacggacg ggtcgtaggt gggcggtacg agcgaggcgg agggcatccg ccggtggccg
     3241 tgctcctcga agaacgcgag gtagcgctcc ctgatctcgg cggccttcac gcggccagtg
     3301 tatccccggg aaatcccctg accggggccc ctaaagcgtc cggagcgggc acgggctcac
     3361 cctccaaaag ctctgcaaaa gctccacgtt cccgcgtcca cgcgcgtgcg cgccgagaaa
     3421 ggacagcgca tggacgtagc tgagcttgtg ggcgaggcga gggagcgcga acgcagcccc
     3481 ctgccgccgc gtgaacggat tgtcgccgga gggatagcca tcaccttcgg gctcgcggca
     3541 tccatcgtcg cgctgaccgt gccactcaac cgcggcaccg atccgctggt actcgcggct
     3601 ctcgtcggcc tcctggcgct cgcccgccgc gtccgcttcg aggtcggcac gctcgaggca
     3661 tgtgcggacc agctcgcctt catcccgctc ctgctgctgg ccccgctgcc gctcgtcccg
     3721 gtcctagtcg ccgccgccta cgtgctcgcg cgcgtgcccg acttcctgaa gggcgggatg
     3781 cacccggacc gctggctcta cttcgtcggc gacgcctggc actcgatcgc cgccgtcgcc
     3841 gtcgtggccg ccctcgcccc cggcgagatc agcgcggagc acctgcccgt gtacggcctc
     3901 gcggtcggcg cgcagatcgc ggccggtctc ctggtcatcg tggtgaacga cacgctgatc
     3961 cacggcttca agccgcgcga gatcttcggc ctctccttct cccacagcgc ccggatcgac
     4021 gcgctgctca gcccgctcgc gctgatcacc gccgcggtgg cggcccagga gccgctcgcg
     4081 atcgccggcg tgatgccgtt ctactggctg atggccatct tctccgccga gcgccgcgag
     4141 cggtacgcgg ccgccctcga gctgaaccag gcctaccgcg gcaccgtgat ggtgctctcc
     4201 gacgtggtcg atgccgagga cagctacacg gccagccact gccgctcggt cgtcgagctg
     4261 gccgccgccg tagccgacga gctcggcatg ggctacgagg accggcagga gctcgagatc
     4321 gccgccctgc tgcacgacgt cggcaagatc gcgatcccca acgagatcct gaacaagccg
     4381 gccaagctga ccgacgagga gttcgccctg atgaagaccc acacgatcga gggccaggcg
     4441 ctgctcgacc gggtcggcgg tcgcctcgcg aaggtcggcg aggtcgtgcg ctcctgccac
     4501 gagcgctggg acggccgcgg ctaccccgac ggcctccagg gcgaggagat cccgcccgcc
     4561 gcgcgcgtcg tgttctgctg cgacgcctac agcgcgatga cgaccgaccg cccctaccgc
     4621 caggcgatgc cgcaggcgga ggcgatcgcg gagctgcgca agaactccgg cacgcagttc
     4681 gagccgcgcg tggtcgaggc gctcgtagcg gtgatcgagg ccgacctggc cggcgccgac
     4741 gacgggcgct acacggacgc gctgcgcgcc gtgctggcgg gcaactcggt gcccgcgcta
     4801 gagctgaccg cgtagcacgg ctccggcgcc gactcttccc acgtcgttcg cagcgcgtac
     4861 caggcccaca ggcagatgag cgccgcgccg ccggaggccc cgagcagcag cgccgtcgcg
     4921 ccgacctgct cgagcgccgc gcccgcaccc acgaacgcga tggcgaacgc ccacgatccg
     4981 gccgtgtcgg cgagcgcgaa cgcgcggccg agcagccggt ccggcacgaa cttctggaag
     5041 atcagccgct cgtgcgtgag tagcagcccg ttccctacgc cggtgagccc gaggccgacc
     5101 agcgccagca ggaacaccgg ggcgatcgcg aacagcgcga tgccgagcgc catcacgagg
     5161 atgccgccga catagcggcg cttga
//
LOCUS       DASVPL010000168        35480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_100253, whole genome shotgun sequence.
ACCESSION   DASVPL010000168 DASVPL010000000
VERSION     DASVPL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 35480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35480
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100253"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1921)
                     /gene="gyrA"
                     /locus_tag="VF517_08675"
     CDS             complement(<1..1921)
                     /gene="gyrA"
                     /locus_tag="VF517_08675"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX8053055.1"
                     /translation="MATADQLGGGNVEPRLLEEEMSSSYLDYAMSVIVGRALPDARDG
                     LKPVHRRVLYAMQLGGLQPNRPYRKCARVVGDVMGSFHPHGDQAIYDTLVRLAQDFAQ
                     RYPLVDGQGNFGSIDNDPPAAQRYTESRLAPLATEMLRDIDADTVDFVPNYDESQLEP
                     SVLPARYPNLLVNGSAGIAVGMATNIPPHNLRETIDATIAYIDDRDIDTAGLMKHIKG
                     PDFPTGGIIVGRTGIREAYETGRGRIVVRARAHIEPLRQGKEAIIVTEMPYQVSKGDG
                     RNDGSGLIKKIAEVVNDKKVPEISDLRDESDKSGVRLVIELKRDAIPKVALNKLYKHT
                     AMQTTFGANMVALVDGVPRTLGLQQLIHNYVDHQRDVVVRRTKYELRRAEARAHILEG
                     LLIALRNIDAVIELIRASRDADVARDGLMEQFELSREQAQAILDLRLQRLTALEAGKI
                     EQEHADLVERIRELREILGDEARVFGLIKEELLEIRERYGDDRRTQITFAEDDLDIED
                     LIADQQMVITITKSGYIKSLPLATYRQQRRGGVGVTGMGMKDEDYIEHLFVCSTHDYL
                     LFFTNRGKVYRSKVYDLPDAPSRQSKGRHLGNVLPLREGERVQSVLATRDFGEAPYLV
                     FATRNGIVKKTAFAAY"
     gene            complement(2045..4465)
                     /gene="gyrB"
                     /locus_tag="VF517_08680"
     CDS             complement(2045..4465)
                     /gene="gyrB"
                     /locus_tag="VF517_08680"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX8053056.1"
                     /translation="MAKESSQEGSSYGAKDITVLEGLEAVRKRPGMYIGSTGTRGLHH
                     LIYEVMDNSVDEALAGECDHVSIAIHPDNSVTVTDNGRGIPVDMHEKEGRPAAEVVLT
                     VLHAGGKFGDGGGYKVSGGLHGVGVSVVNALSEKLELTIWRDGYEWNQSYERGAPQTE
                     LKRGAKSDRRGTSITFLPDLEIFETIDYDRSTLEQRFREMGFLTKGLRIDFSDERGEG
                     FSTTFKYDGGIRDFVTYLHSQGTKEPLHKKIAYLEGADENVGEVEVALQWNNSYQESL
                     LSFANNINTHEGGTHLSGFRSALTRTINAYARQIGELKEKDDNLQGEDVREGLTAIIS
                     VKVQEPQFEGQTKTKLGNPPVEGFVQQAVNKGLAEFLEENPQDAKSVIKKAVQASRAR
                     EAARKARDLTRRKSALENSTLPGKLADCSVRDPALAEIFVVEGDSAGGSAKQGRDRNT
                     QAVLPLRGKIINAEKNRIDKVLSNQEIQAMITAIGTGIREEFDIERARYHKVIVMCDA
                     DVDGAHIRTLILTFLFREMRDLIEAGYVYLAKAPLYKVKSGKTELYIERESELEEFLL
                     RDKIEKIEVFDREGAQFKLTHARWQKYVRLLKQYEGWASSLRAEYGHDTITFLEESQI
                     LDEGATDASAVIALTNKADPEGEPYETELMSEDDTELRIRVVERSTGSAATYRLKRAL
                     FESSDYRGFVRVHADLKALAGIPPFAVRLGKKEERALSFEDLRRAVLDVAKEGVHLQR
                     FKGLGEMNADQLFDTTMDPSRRTLQQVTIDDASAADQIFSMLMGDKVEPRRLFIEAHA
                     REVVNLDV"
     gene            complement(4721..5041)
                     /locus_tag="VF517_08685"
     CDS             complement(4721..5041)
                     /locus_tag="VF517_08685"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX8053057.1"
                     /translation="MRRRDPRPLASALAGVVDELAPATTLARVQSCWPAAAGAAIARE
                     AEPVAERDGVVTITCTSSVWAQEIELLGGDLVGRLNNALGAAEGPPPVRSLKVSASPR
                     RARR"
     gene            complement(5038..6147)
                     /locus_tag="VF517_08690"
     CDS             complement(5038..6147)
                     /locus_tag="VF517_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX8053058.1"
                     /translation="MHVTGLALRDFRNYERAEVELGPGLNVAAGPNGAGKTNLLEALY
                     VGLTGRSCRTSVDRELVRIGQKVARVEARVEAPDGEHALEVGIAPGDEKRIRVDGAPL
                     QRGVDPPRPLAAVFLPDRLDLVKGAPALRRAHLDQVVVALWPARVATRSGYTRALAQR
                     NALIARVRAGAATAEQLDVWDAELARHGAQLMADRAAAVELVAPAFAARAEELGLPER
                     AGLRYAPRSKAGDADGLRAELVERRAADLDRGFTTHGPHRDDLALTHGGRALRTLGSQ
                     GQQRLGLLALLFAERDALAARGTPPLMLLDDVMSELDAGRRARLSDLVRAEGQALITT
                     TDAEHVPGADASDVVLFEVTTGSVERAGARGLRAA"
     gene            complement(6157..7275)
                     /gene="dnaN"
                     /locus_tag="VF517_08695"
     CDS             complement(6157..7275)
                     /gene="dnaN"
                     /locus_tag="VF517_08695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX8053059.1"
                     /translation="MKLSIGKEQLLSALGIAARGASTRSAIQTLAGVMIRAGDGRVEL
                     QATDMELGVRVNVDVAPDRDGVAVVPARLLLDVVRSLPRDDVTLEYRSAQGDVEVVSG
                     PARFHLRTLPADEFPNLPEIGDAAVVRVPARAFVETVGRVARAASRDETRPHLTGVLV
                     SAAEKELRMVATDSYRLSVKETALEQALDGTLEANVPARTLQELSRVAGTEADGQIEI
                     ASLEHQVVFRVGPVTLSSRLVEGRFPNYKQLLPETFEHELRVSGAEIIEVVRRISLLA
                     QKNAPLRMKFSDGQLEISAQTPDIGEASESLPIPFKGEQLEIGFNPDFLREGLESVDA
                     DELIFKLISPLRPGLIEAVAGDEGGSFIYLVMPIRLNV"
     gene            complement(7390..8778)
                     /gene="dnaA"
                     /locus_tag="VF517_08700"
     CDS             complement(7390..8778)
                     /gene="dnaA"
                     /locus_tag="VF517_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX8053060.1"
                     /translation="MPGDLEPPSPNDLSEMWEAVVRELRGEVTDSTFHIWLAPLGAAG
                     NSSGTLFVRAPDHIRGWVQERFLGVIRRAASRAGVAGAVEVVDERWQAPAAETPRPAR
                     ATAAASEDGEPAAAGLNPRYTFDQFVICDGNRLGHGAALAVAEQPGQAYNPLFIHGPP
                     GLGKTHLLHAIGNYVRSYGDGLRVRYATVDTFTGDFVKAVRSGNTRAFRARFRDADVL
                     LVDDIQFLAEKLRTEEEFFHTFNALYEAGSQLVITSDRAPRDIEALEARLRERFECGL
                     VAALEAPDFEARLAILQKRARIDALGNVPAATLAEVARRVTSSVRALEGALIRVVAYA
                     SLRGEEPGPELAARVLGTLYSSSEPAASTTLGQIQAAAATALDVTPESLIAHDRRPKV
                     ALARQVAMYLAREMTEESLPAIGRHFGGRNHSTVIHAHRRIAGDIGSRDETRAAVDRA
                     RDVLGGQPADRC"
     gene            9044..9178
                     /gene="rpmH"
                     /locus_tag="VF517_08705"
     CDS             9044..9178
                     /gene="rpmH"
                     /locus_tag="VF517_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX8053061.1"
                     /translation="MKRTYQPKKRKRARTHGFRARMQTRAGRHMLKRRRDKGRRKLTP
                     "
     gene            9181..9570
                     /gene="rnpA"
                     /locus_tag="VF517_08710"
     CDS             9181..9570
                     /gene="rnpA"
                     /locus_tag="VF517_08710"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX8053062.1"
                     /translation="MAAAHGGGRRRGRRQRLSRSAEFDRVFREGRSHASRQLVLYAFP
                     NAESGEEPRLGVSVGRKVGGAVQRNRVKRLLREAFGALSAELPGGHDFVVVARPDAAA
                     FAVDGGEQAVEGALRELLEQAGLVTAA"
     gene            9567..9809
                     /gene="yidD"
                     /locus_tag="VF517_08715"
     CDS             9567..9809
                     /gene="yidD"
                     /locus_tag="VF517_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX8053063.1"
                     /translation="MKALVLAPLHLYRRVISPLLPARCRYYPTCSQYAIEAVREYGVI
                     RGSILAAWRVMRCNPLSHGGFDHVHDQRLFRQSQAV"
     gene            9813..10805
                     /locus_tag="VF517_08720"
     CDS             9813..10805
                     /locus_tag="VF517_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX8053064.1"
                     /translation="MYPVLANVLQPLINVNEAILNFFHGVTNSWGVAIIGLTVTVRLA
                     ILPLTFRQVKGMQQMQRLAPEMKKIQERFKDDRQRQQQELMKFYKEHNFNPLSSCLPL
                     LLQLPFFLSLFYLQRQQEFKDEVRETGKEFLFIPDLTSKATGAVLVVLIVTYVVTQLG
                     SSLVTAVNIQDKNQKRLMLALPFVFTVVIVKFPTGLLVYWITTNLWTIGQQLAVRRFM
                     PAPAPVKSIDDKDSGDGGGGGGGSGGKAKRRQGMMERAAARMAGEDGKSGDGDGDRAG
                     KATGKAKGAGGGGAAKSSGATAKGSGGSGGGNGGPRKAPPPSPRKKKKRSGRKR"
     gene            10841..11356
                     /locus_tag="VF517_08725"
     CDS             10841..11356
                     /locus_tag="VF517_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX8053065.1"
                     /translation="MVPGEDDAPARADGEAPPDEPQERVRALVGRVVQGLGLTASVDV
                     EVDDEAIRATVNGDDLGILIGKHGSTIDALQHLALRVAYKGGAAERQVVVDAAGYRER
                     RETTLQRAADRAVSDALDFGRPVELEPMTASERRLVHTYLRDRNDIQTHSEGDEPDRR
                     LVVSPVRSHGS"
     gene            11486..12154
                     /locus_tag="VF517_08730"
     CDS             11486..12154
                     /locus_tag="VF517_08730"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX8053066.1"
                     /translation="MDPTHADTLERILAALDAEPDPHTSVSPGEAVDVHIADSLAGLA
                     VPALRSAHTIADLGAGAGFPGLVLATVLAHARVDLIESASRKTAVIDRLAAAGGLQNA
                     RAVTARAEDWAAAEGREAYDTVTARAVAPLAVLVEYAAPLLRPGGVLVAWKGAREDQG
                     EAAGAAAAALVGLRPADVLPATPYPASRNRHLNVFEKVAPTPPRFPRRAGMAAKRPLG
                     GAGR"
     gene            12656..13414
                     /locus_tag="VF517_08735"
     CDS             12656..13414
                     /locus_tag="VF517_08735"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX8053067.1"
                     /translation="MGIVYAIANQKGGVGKTTTAVNLAACVADAGYETLLVDLDPQCN
                     ATVGLGARKDLEPNVYTCLAGDAPLHDAVIRTDVPGLSLAPAAPDLAGASVELPREPG
                     SETRLRDALDGFRDRYLFTILDCPPSLGPLTVNALVAADRVIVPVQTEYFALEGLAGL
                     LDTLSLIQREMNPRLSVSGLVLTMHDGRTRLAQDVERELREHFPELVFDSVIPRNVRV
                     GEAPSFGVPVTRHDPACSGSVAYLKLAREVAARG"
     gene            13407..14345
                     /locus_tag="VF517_08740"
     CDS             13407..14345
                     /locus_tag="VF517_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX8053068.1"
                     /translation="MAEPARIPEPEATRTGEPEAIAAGRQRGMGRGLAAILSTGPRPE
                     VGLRELAVELIRPNPAQPRSEFDEDALLALSRSIAARGVLQPIVVRPLPGGTYELVAG
                     ERRLRAARLAGLGTIPAVVRETADGDRLELALIENMAREDLNPIEEARALATLVDDLG
                     LSKEELGRRVGRSRPAISNMIRMLELPDEALAMVERGELSAGHGRALLLCRGHDERLR
                     LARRAREESWSVRVTEDRAREAEDGGRAREPRGPVVIHPDLADSLAAAEDALSAALGR
                     EVRVKPRAGGYRVEFDLDHPREGVDLAERILRRNGA"
     gene            14371..14444
                     /locus_tag="VF517_08745"
     tRNA            14371..14444
                     /locus_tag="VF517_08745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14405..14407,aa:Ile,seq:gat)
     gene            complement(14456..15055)
                     /locus_tag="VF517_08750"
     CDS             complement(14456..15055)
                     /locus_tag="VF517_08750"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8053069.1"
                     /translation="MSKVTRVTAKRAVTERRAATHARLVDAATKVVARRGFHAATVDA
                     IADEAGYSVGALYSNFKGKDDVFLAVFDGHLRWYTDQLEAARDAPVPAQAFRDWLDGL
                     TRDPEQLLVFIEFWAYAVRKPKLRRHFASRMRGIRATLAGMIDERAEANGAELPIPSA
                     TLAVVLLALWRGLALEKLADPEAVDDAVTDLVASLVPPR"
     gene            15120..15995
                     /locus_tag="VF517_08755"
     CDS             15120..15995
                     /locus_tag="VF517_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8053070.1"
                     /translation="MAQGAEVREGTVRANGIEFATIEAGEGPLVLLLHGFPDNPWTYE
                     AQIGELAANGYRAVAPHMRGYPPTGSAPDGRYDSEALAGDIAGLVRALGDGPAYVIGN
                     DWGAIATYAAMSLHPETISRAVVINVGHPSTFLTTLLHPHQVHHIFHFWFFQLGPIAT
                     GAVRANDLAFVDYLWDYWSAPGHDYADHLARVKRTLEPEGAVEAATSYYSGLLNMPTA
                     QPDAAARMQGRITVPTLSIFGDHDPVRELSRDEHEHFDADYRLEIVEGAGHFVHRERP
                     DQVNRLVLDWLGAAR"
     gene            15992..16852
                     /locus_tag="VF517_08760"
     CDS             15992..16852
                     /locus_tag="VF517_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8053071.1"
                     /translation="MTYDDYKSSTRTTTTPHGEFAYLDAGEGPPALFVHGLFMSGYFW
                     APVIGAVKDVRRCIAYNLPDHGGTTVAAEQDLSLDANAEMLEAFCDSLGLDRFDLVAN
                     DTGGAIAQALAVRIPDRVKTLTLTNCEARDWMPSKHDLGQLVKSLAEQGQLAQVLAAN
                     YEDRDAARSTAFAATFQWPDRPSDDDVRGLMEPHQTTLDAAKRLERFAAAIEPNELMA
                     IEPRLRELTVPSLAVWGTGDPIFPLELAHWLRDTIPSLDEVVEIEGGLLFWPWERPDE
                     LAPHLRRHWT"
     gene            17000..17755
                     /locus_tag="VF517_08765"
     CDS             17000..17755
                     /locus_tag="VF517_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HEX8053072.1"
                     /translation="MAWNKRFDRQNGAAGEDPLHGNVATRTRDPLPQQAPPPDPEDGI
                     APRRERTHPVDDRPHTGRRAAATAATTTTGHGARDRFGGLNWGAAFFGWLTALGMAAI
                     LTALLSAAGAAIGLTEGSAAGSAEDNAETVGIVGGVLLLAVLGIAYFCGGYVAGRMSR
                     FDGKRQGFGVWAIGFLITIAIAVVAAVAGSEWNVFNQLNLPRIPIDEGDLATGGAIAL
                     ALGLLTTLIASILGGAKGRHYHDRVDRADGRDW"
     gene            17828..19177
                     /locus_tag="VF517_08770"
     CDS             17828..19177
                     /locus_tag="VF517_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8053073.1"
                     /translation="MNTSSRIALTASALTLVLPATAGASTIEVSASAGPAASEFLQYR
                     ASKGEANRVRVLFGKNSVVLVDKSTKRITVKRDQGFGRCRATSRHRVVCPDMPLFASL
                     RDSKDRFSVAPGDRGDAPTSTNPRSYAADYEDTEGAVTVTLFVDAGSGDDSVTGSKYD
                     DTILPGPGRDTVDGRDGPDAIDSEPDGVIDSLDGGGDIDSISFFSSAPVSVDLAAGAG
                     GPAGELDRLAGFERVHGGSGDDDIRGTETTDALYGESGRDHLDGRGGNDMVVGDAPDV
                     TNGSPNELIGGDGDDVLDTRAEKPAPTSTVACGAGNDSHIGGVDALLDPSCESALLRV
                     GFGTVTDPETEEVLYDDPMKVTPVAKTSDSITFEVPCPKHRNGACTGKLELPDYGSAD
                     FSLSPGERRSVTVPLNAAGQAAVAGSSPVAVRLSMDLSPVNQGQFVARTSLGFQADL"
     gene            complement(19210..20268)
                     /locus_tag="VF517_08775"
     CDS             complement(19210..20268)
                     /locus_tag="VF517_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8053074.1"
                     /translation="MRVVVVGATGNVGTSVLRSLVDEPEVTEILGIARRLPGMQWPKT
                     SFVEADIVSDDLVPHFQGADAVIHLAWLIQPSRDEEQLHAVNVDGSGRVFRAAGEAGV
                     GALVYASSIGAYSPGPKDRGVDESWPTDGIDTSFYSRHKAATERQLDQIETDFPGMRV
                     VRLRKGLVFKREAAAEIRRYFIGPLLPGSLMRPQLIPVVPDIEGLVVQAVHSYDAGEA
                     YRLAVVRDVRGAFNIAADPVIDPDMLAELFGAKKVSIPPAAVRAAADASWQAHAQPTP
                     PGWVDLALNVPIMDTTRARDVLGWEAKHSSGEALMDLLNGLRDGSGADTPPLAPESSG
                     PGRVREFMTGVGSRNPTD"
     gene            complement(20270..20929)
                     /locus_tag="VF517_08780"
     CDS             complement(20270..20929)
                     /locus_tag="VF517_08780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8053075.1"
                     /translation="MASTAILDIDGTLVDTNYQHAVAWYRAFRQHDVILPIWRIHRHL
                     GMGGDQLVESLTDKQTDEEKGDDIRTAEKALYLALIEEVEPLAGARDLIKRLKDDGRT
                     VVLASSAKGNEVEHYLDLLDARELADDWTTSDDVEATKPEPDLVKAALEKAGAEPGDA
                     VMLGDTPWDCKAAAAAGVETIALITGGFSEDELREGGAAAVFESIPELLERLGETPLG
                     G"
     gene            complement(20929..21669)
                     /locus_tag="VF517_08785"
     CDS             complement(20929..21669)
                     /locus_tag="VF517_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8053076.1"
                     /translation="MLRRDMAEGIHRIQDGYVNWYLVEDGDSLTVVDAGTPAGWKLLE
                     DAVRQLGRTLSDVKALVLTHAHYDHVGFAERIRRELGIPVWVHEDDVSLSKHPAFFRT
                     ERLPVVYLKDRGLRKVGLDFLRRGAFFARPVKEVRTYAGEGPVDVPGRPHAVFTPGHT
                     KGHVAFHFPERDLVIAGDAIVTFDPYTGSTGPRLVAKGATNDTEQALRSLDKIGALDA
                     GTVAVGHGDPFEGGAAEAARLARAAGNE"
     gene            complement(21700..22749)
                     /locus_tag="VF517_08790"
     CDS             complement(21700..22749)
                     /locus_tag="VF517_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053077.1"
                     /translation="MARLRRVDCSSPGITRRRRGRGFVFVDEDGDRVDDPEVLGRINE
                     LAIPPAWQDVWVCPYPMGHLQATGVDARGRKQYLYHPKWRERQDQLKFDEMIGFARAL
                     PGMRERVAHDLDSDWLSRPQVLACSVRLLDRGFFRVGGEDYAVENESYGLATIRKEHV
                     RVGDGTITFDYPAKSGQRRVQSIVDDQVYEIVAALKRRRGGVGPELLAYKQGTRWRDI
                     KSADINVYVKEVTGGDFSAKDFRTWNATVLASVALAVSGPAATMSKTARNRAKVRAVK
                     EVAHYLGNTPAVCRASYIDPRVFDRFDSGLTIGGVLQEVADTNDLDSLHGPVEEAVLD
                     LIAEEESSPAIERVA"
     gene            complement(22749..23564)
                     /locus_tag="VF517_08795"
     CDS             complement(22749..23564)
                     /locus_tag="VF517_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8053078.1"
                     /translation="MGSPDSPSEDWLGLCRRAAASARAAVAPFDDPAARARETWRGEG
                     GDMALAIDRAAEDAVLEVLEESGVGARVVTEERGEVEVGGGGPLVVVLDPIDGSLNAK
                     RAMPWHAVSIAVASGPSMADVDFGYVATFDGAEEWWARRGGGAWLGDERLDVTRSTRE
                     LEVLGVESARPDLVAGAADLLAATGARRLRMIGSIALSLCYVGGMRFDAMLSLRSCRS
                     VDAAAAQLVVREAGGVVSFPDAGGEDLSGSLDLDMRSRVTAATHPELLAAVSG"
     gene            23587..26730
                     /locus_tag="VF517_08800"
     CDS             23587..26730
                     /locus_tag="VF517_08800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX8053079.1"
                     /translation="MPQRTAELSAAQREAVNHRGTPLLIRGGAGTGKTRTLLERFGAL
                     VREGEPADSVLLLAFSNAAAAEARERLETSIQTPWEELWACTFRSFCARLLREEALEA
                     GIDPFFVPVTPADRLALLLDRIDELHLRRHEIRGNPAPLLGGFVTRIDRLKEEMIRPA
                     DYRAHAEGLAELARDDADRANAARELELAAVYERHDQLLAQRGALDAGDIVLRAFDLL
                     LERPHVRRGLGERFRHMLVDEYQDVNFAQAAVLRLLAAEQRDVAVAGDPDAAIHRLRG
                     AGAKNLDDFEREQPDALVIRLDRSQRSGRRIVTAAQAVGGPDTRLTARAGGEVTFWHC
                     RSDRAQAQRAALEAEQRITGGVPPDQVCVLVREVAGEGALVGAALEERGIPFRLAGAA
                     AYFQRSEVRDVIAWLRLLADPGDSGAVVRALSRPPIELRQVDIARLTQLARRRKLDMV
                     SGIEAACEAPGLSPEGRDRALAFLRVYRAASRAFEEMSPDRFVHRLIERIGLRRQQVF
                     AAKADTVERLVNIAKLADLAVEYVRREPGSSSRDFARYLAAVAEAGLPEEEAAPGNHA
                     GSAVQVMSMRDAKGTEFDTVLVLGLSAAAMPGSRREGGHRIPAALLKEDLPEPDPREE
                     HEAAMRRLLHVAVTRARRRLVLSWPEGDRGPGARPSPYFEDARAAVEAEVEVVEEELF
                     GPAEGLHSTFRMMRDELLDSVAHVGGRLGEMRLDTYLEVSRSVARYLELLKIAALIER
                     GKAGQSVNDALAEVNELLLQGASPEQAELFRLSALDDYLLDTAGSDERRRREAIVATP
                     DDTLEPFIPRRGEGLMLSASDIETYRLCPLKYKFARVFRIPQEPSINQRFGIVVHQVL
                     ERFHATDGASLETLMQLFESAWRRSGFGDSNDDLQFRERALAALRRYWELDRRRESEP
                     VSFERSFSFHIGPHLLRGRVDRVDRLPDGRHELIDYKTGKAKTEQDLREDVQLSIYQM
                     GAREAWGLETAAQSYYYVLENEKVPVAHSEEELERVRGTVTEIADRIMAHRFEPKPSF
                     ELCSFCDYRIVCPAAEK"
     gene            complement(26697..27458)
                     /locus_tag="VF517_08805"
     CDS             complement(26697..27458)
                     /locus_tag="VF517_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053080.1"
                     /translation="MNLRRLVREYVPDAAMVADLVGRREPPKEAGEALFMQAPSLGLQ
                     YTSGGGYPPVTPLLGAGERRHFEHTLSGPLFGRLGGPTCVLGHYTYEIAIEDHEAYVE
                     RHDWVPHRFTVCAAEIGGPLGRLRGVYLRPRLSAIKLEDDWLAVGSPRHRVKLESGRF
                     NDIYDLHRADGQDEVALRELFSPSFVVWLQEHPLKPGFECGAGTLVVFVRGYETAAGK
                     ITFLHEATRDIARRIERQLGEAAGPHFSAAGQTIL"
     gene            complement(27455..28015)
                     /locus_tag="VF517_08810"
     CDS             complement(27455..28015)
                     /locus_tag="VF517_08810"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX8053081.1"
                     /translation="MSDTVFYAIGGGLILLIVWIVVTWNSFVSARNKVEEAWSGIDVQ
                     LKRRHDLVPNLVETVKGYAAHERETLTEVTRARGAAMAASDPADVARTEGALTAALGD
                     VRALAEQYPDLQAAANFRGLQSHLAQLEDEIQASRRIYNSNVQTYNTRVQVFPNVLLA
                     GSSFPARAFFVLDSPAERLVPAVSFA"
     gene            complement(28052..28927)
                     /locus_tag="VF517_08815"
     CDS             complement(28052..28927)
                     /locus_tag="VF517_08815"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX8053082.1"
                     /translation="MDHVEWSERPVLRRPALVVAFKGWNDAGEAATAALDYLVDSFEA
                     TEFARIDPEEFYDFTAVRPNVHLEEGLTRVIEWPENSFHAAQVAGAEHDLILFEGVEP
                     SLRWGTFTQVLIDMARELGAESVITLGALLADVPHSRPVPMTGIASDQALVEKLGFER
                     STYEGPTGIVGVVHHACAAAGIPSVSLWASVPHYVAAAPNPKAALALVRSFEGAAGVA
                     VEARELEESAEDYERQVSAAVASDPDVKSFVERLEQTMDEAVEEMPSPRIPSADAIAR
                     DFQRFLRQRGPETGS"
     gene            29003..30181
                     /locus_tag="VF517_08820"
     CDS             29003..30181
                     /locus_tag="VF517_08820"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX8053083.1"
                     /translation="MEPSFRRTLYALIGCGVLMRLAWVAQTGGLPFDMQSFRAVSGAL
                     LDQPLRLYGELNPPGMYRWPYPPGYFPWVLASHGLDSSGLLGFRAAIVLPAIAADAAL
                     AWIVQAALGRGGASERTRLGAAALVALGPSFALISGYHGQIDAVAILPAVAALLVWER
                     GGSRRALAAGLLIGLGASVKSAPGLVLLALLPTVASRREAATLIGAAVAVPLAALAPF
                     LAADASGVVRALGYAGIPGEGGLTMIVDPDLARYWVQNAKDVRPNGLSSFLSQHGSLF
                     TGAALLAAAGLLVRHRVEPRRAALILWLTFYVLGTGFAFQYVIWGLPFLILAGRLRWA
                     FALQAALLVPTLIYYTGPFPALDVLVPVYFVLMAAVWLGLAAALAVTALDRRRAPVTA
                     "
     gene            30193..31125
                     /locus_tag="VF517_08825"
     CDS             30193..31125
                     /locus_tag="VF517_08825"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8053084.1"
                     /translation="MAGRLAAAVLASAASLTLSACGGGSGGEPERVVVALDFTPNAVH
                     APIYAARDEGIEVRTPGSAPDALKLVAAGRADIGVLDIHDLALAVEKGSDVVGIAALV
                     QRPLAAIIARGNVRRPRDLEGRRVGVSGLPSDPAVLRAVLEHDGGRLGAVDQVTIGFA
                     AVPNLVQRKVDAVPAFWNAEGVTLRRRGVDIREFRVDDYGAPRYPEVVLFTSRRALRE
                     HRGKIEHALRAIRAGFDSVEQRPGAAAHAIARLSGADVELVRAQLDAVAPALRPPLRL
                     DRPLLERWADFDARFGIVHRRPDVGRLFDFGIAP"
     gene            31122..31823
                     /locus_tag="VF517_08830"
     CDS             31122..31823
                     /locus_tag="VF517_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX8053085.1"
                     /translation="MTAVAPWPPPAVPALEEGDVQVWSLDLDAEPDASLLDAGERERA
                     GRFVSDELTRRWTNARAGLRGVLAAYCGADATQLRIESAPCVHCAEPHGKPFLAEPSG
                     HDLRFNLSHSGTVGLVAVARGREVGVDVEAIRPGRRADGIASRWFTPEESDHLRGLGE
                     AEAELAFYRLWARKEAYLKATAAGVPGGLDSFDALHLELASGAEPGWEFADLDVGAGY
                     AAALAVAPPGYRPGG"
     gene            complement(31805..32605)
                     /locus_tag="VF517_08835"
     CDS             complement(31805..32605)
                     /locus_tag="VF517_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053086.1"
                     /translation="MSRLVRLPLRAPLFAKLLVVVLLAAGAAAALAAQPARDDATAPA
                     PPANPGRGEVVSLSDGPGTPTPVAHVRDPEESLSHPDPEAAQDGPKAKAPHVAGASSD
                     DGVSPGAPSDEEVREMLREMREGGGGPAGSVTLTKGGLARVPSNAPQAVRAVIAGGNA
                     IADFPYIWGGGHGSFQDSGYDCSGSVSYALAAGGLLKAPMASGNFMKWGEEGRGKWIT
                     IYSNPGHMFMVVGGLRFDTSGRSGARGSRWQAAPRSFGGFVARHPPGL"
     gene            32710..33198
                     /locus_tag="VF517_08840"
     CDS             32710..33198
                     /locus_tag="VF517_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053087.1"
                     /translation="MHARITATAAAALATGALALAGCGSDSGPELKVGACTNADPTEA
                     LSFDVKAVDCGDDEAKSKVVKQVEKSNQCDVASVSDSANDKVYCTEPYPPGSAPTKPE
                     VGACTTADPNQLVSVNIRLVNCDDKEAKSKIIERAAQEDECKKGSIRGSGGEIFCIAP
                     VR"
     gene            complement(33195..34070)
                     /locus_tag="VF517_08845"
     CDS             complement(33195..34070)
                     /locus_tag="VF517_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8053088.1"
                     /translation="MDAVVARPGEGEIIVDHPAGGIVIKGAHEQVVVSEVRLSPGTPG
                     PPPHVHHHHSDAFYVLEGTLTVRLGGEERPLHPGGFVRIPPNVVHTVGNADEGAPVRA
                     LNLHAPSMGFDDYLRAGRDGDQAARERFDQHDPPADGGRPASDARVLDAGEGEPLRFG
                     PTSAVFKATGDDDAGHFSLSETTAAPGFPGPVAHVHRTFVDSFYVLEGTLTVRLGEET
                     LGLDAGTFALVPPGTVHTFSNPGDEPVRFLNLMAPGGFERYLREVVAAATSGGPPDQA
                     LMAEIASRYDFEPAA"
     gene            complement(34075..35400)
                     /locus_tag="VF517_08850"
     CDS             complement(34075..35400)
                     /locus_tag="VF517_08850"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEX8053089.1"
                     /translation="MRTRTAALVPLALLLLAPADALAAAGGGSSGFGGGGGGGGGGGY
                     SGGGGSGSGEGSFLDFLIFAVIIAVIFVGAMIVNALERRRILKRDERVTTASAEAAQD
                     DAYLAADAVKRDAAELFKQIQVAWSAKDRERLNAMVGSDLMKEWSKRLDDFDRKGWHN
                     VVNVKSGPKCLYVGLVNREDDTEDRVVVRVEATLEDYVQMKGGGRMMSDGESDETREL
                     AEWWTLCRTADDGWMLLSIEQDDEGMHHLKSDVVATPWADDQRLRDESLVEGAVANRV
                     AEGFTVAEVADLDFDGEARAAALDLSLADARFGPDVLEVAARRAVAAWAEAVDGEDRE
                     LEAIAEPAALGDLLHPGDPSRTTRLVVRGPRITRLHIAALDAAAQPPTMTLELDVTGT
                     RYVQNRDTAAVVAGSDTSETTFTERWTMALSGSDDQPWRIASVAGAAIR"
BASE COUNT         4836 a        12906 c        12651 g         5087 t
ORIGIN      
        1 tgtaggcagc gaacgcggtc ttcttgacga tcccgttgcg cgtcgcgaac acgaggtacg
       61 gcgcctcgcc gaagtcccgc gtggccagca ccgactgcac gcgctcgccc tcgcgcagcg
      121 gcagcacgtt gccgaggtgg cggcccttgg actggcgcga gggcgcgtcc gggaggtcgt
      181 agaccttcga gcggtagacc ttcccgcggt tggtgaagaa gagcaggtag tcgtgcgtcg
      241 agcagacgaa gaggtgctcg atgtagtcct cgtccttcat ccccatgccg gtcacgccca
      301 cgccgccgcg gcgttgctgg cggtaggtgg ccagtggcag cgacttgatg tagccggact
      361 tggtgatcgt gatcaccatc tgctggtcgg cgatcaggtc ctcgatgtcg aggtcgtcct
      421 ccgcgaacgt gatctgcgtg cggcggtcgt cgccgtagcg ctcgcggatc tcgagcagct
      481 cctccttgat cagcccgaac acgcgcgcct cgtcgccgag gatctcgcgc agctcgcgga
      541 tccgctccac caggtcggcg tgctcctgct cgatcttgcc ggcctcgagc gcggtgaggc
      601 gctggaggcg caggtcgagg atcgcctgcg cctgctcgcg cgagagctcg aactgctcca
      661 tcaggccgtc gcgggcgacg tccgcgtcac gcgaggcgcg gatcagctcg atgaccgcgt
      721 cgatgttgcg gagcgcgatc agcagcccct cgaggatgtg cgcgcgcgcc tcggcgcggc
      781 gcagctcgta cttcgtacgg cgcaccacga cgtcgcgctg gtggtccacg tagttgtgga
      841 tgagctgctg gagcccgagc gtgcgcggta ccccgtcgac cagcgcgacc atgttcgcgc
      901 cgaacgtcgt ctgcatcgcg gtgtgcttgt agagcttgtt gagcgcgacc ttcgggatcg
      961 cgtcgcgctt gagctcgatc acgaggcgca cgccggactt gtccgactcg tcgcgcaggt
     1021 ccgagatctc gggcaccttc ttgtcgttga cgacctcggc gatcttcttg atcagcccgc
     1081 tgccgtcgtt gcgcccgtcg cccttcgaca cctggtaggg catctcggtg acgatgatcg
     1141 cctccttgcc ctggcgcagc ggctcgatgt gggcgcgcgc ccggaccacg atgcggccgc
     1201 ggccggtctc gtacgcctcc ctgatccccg tgcgcccgac gatgatgccg ccggtcggga
     1261 agtccgggcc cttgatgtgc ttcatcagcc cggcggtgtc gatgtcacgg tcgtcgatgt
     1321 aggcgatcgt cgcgtcgatc gtctcgcgca ggttgtgcgg cgggatgttc gtggccatgc
     1381 cgacggcgat gccggccgag ccgttgacga gcaggttcgg gtagcgcgcc ggcaggacgc
     1441 tcggctcgag ctgcgactcg tcgtagttcg ggacgaagtc gacggtgtcg gcgtcgatgt
     1501 cgcgcagcat ctccgtggcc agcggcgcga gccgcgactc ggtgtagcgc tgagcggcgg
     1561 gcgggtcgtt gtcgatcgag ccgaagttgc cctggccgtc gaccagcggg tagcgctgcg
     1621 cgaagtcctg cgcgagccgt acgagcgtgt cgtagatcgc ctggtcgccg tgcggatgga
     1681 acgagcccat cacgtcgccg accacacgcg cgcacttgcg gtacgggcgg ttcggctgga
     1741 gcccgccgag ctgcatcgcg tacagcacgc ggcggtgcac cggcttgaga ccgtcgcgcg
     1801 cgtcgggcag cgcacgcccg acgatcacgc tcatcgcgta gtcgaggtac gacgacgaca
     1861 tctcctcctc gaggagacgc ggctcgacgt tgccccctcc gagttgatcc gcggtggcca
     1921 ctagccggcc ctccaatcac acacgggtcc gaaaaccgag gaatccaagg acgcagggag
     1981 cgacgcgtgc gatgagcacg cgagcgaccg aggacgccgg agtccgaagg tttgcagggc
     2041 ccgctcagac atcgagattg acgacttcgc gggcgtgagc ctcgatgaac aagcgccgtg
     2101 gttcgacctt gtcgcccatc agcatcgaga agatctggtc cgcggcgctc gcgtcgtcga
     2161 tcgtcacctg ctgcagcgtg cgccgcgacg ggtccatcgt cgtgtcgaag agctggtcgg
     2221 cgttcatctc gccgaggccc ttgaagcgct ggaggtgcac gccctccttc gcaacgtcga
     2281 gcaccgcgcg gcgcaggtcc tcgaacgaca gcgcgcgctc ctccttcttg ccgagccgca
     2341 ccgcgaacgg cggtatcccg gcaagcgcct tcagatccgc atgcacgcgc acgaagccgc
     2401 ggtagtcgct cgactcgaac agcgcccgct tgagccggta cgtggcggcc gatccggtcg
     2461 agcgctcgac cacgcgtatg cgcagctcgg tgtcgtcctc cgacatcagc tcggtctcgt
     2521 acggctcgcc ctcgggatcg gccttgttgg tcagggcgat gaccgcgctg gcgtcggtcg
     2581 ccccctcgtc gaggatctgc gactcctcta ggaacgtgat cgtgtcgtgg ccgtactccg
     2641 cgcgcagcga cgacgcccag ccctcgtact gcttcagcag ccgcacgtac ttctgccagc
     2701 gcgcgtgcgt gagcttgaac tgcgcgccct cgcggtcgaa cacctcgatc ttctcgatct
     2761 tgtcgcgcag caggaactcc tcgagttcag actcgcgctc gatgtagagc tcggtcttgc
     2821 cggacttcac cttgtagagc ggcgccttcg ccaggtagac gtagccggcc tcgatcaggt
     2881 cgcgcatctc gcggaagagg aacgtgagga tcagcgtgcg gatgtgcgcg ccgtccacgt
     2941 cggcgtcgca catgacgatc accttgtggt agcgggcccg ctcgatgtcg aactcctcgc
     3001 ggatcccggt gccgatcgcg gtgatcatcg cctggatctc ctggttcgac aggaccttgt
     3061 cgatccggtt cttctccgcg ttgatgatct tgccgcggag cggcagcacc gcctgcgtgt
     3121 tgcgatcgcg tccctgcttc gccgacccgc cggcggagtc gccctcgacc acgaagatct
     3181 cggcgagcgc ggggtcgcgc accgagcagt cggcgagctt gccggggagc gtcgagttct
     3241 cgagcgcgga cttgcgccgg gtcaggtcgc gcgccttgcg ggcggcctcg cgcgcacgtg
     3301 acgcctgcac cgccttcttg atgaccgact tggcgtcctg cgggttctcc tcgaggaact
     3361 cggctagccc cttgttcacg gcctgctgca cgaagccctc gaccgggggg ttgccgagct
     3421 tggtcttcgt ctggccctcg aactgcggct cctggacctt caccgagatg atcgcggtga
     3481 gcccctcgcg cacgtcctcg ccctggaggt tgtcgtcctt ctccttgagc tcgccgatct
     3541 gacgcgcgta cgcgttgatc gtgcgcgtga gcgcggagcg gaagccggac aggtgggtgc
     3601 cgccctcgtg cgtgttgatg ttgttggcga acgagagcag cgactcctgg tacgagttgt
     3661 tccactggag cgcgacctcg acctcgccga cgttctcgtc agcgccctcg aggtaggcga
     3721 tcttcttgtg gagcggctcc ttcgtgccct gcgagtgcag gtaggtgacg aagtcgcgga
     3781 tgccgccgtc gtacttgaag gtggtcgaga agccctcgcc tcgctcgtcg gagaagtcga
     3841 tccgcagccc cttcgtgagg aagcccatct cgcggaagcg ctgctcgagc gtcgagcggt
     3901 cgtagtcgat cgtctcgaag atctcgaggt ccggcaggaa ggtgatgctg gtgccgcggc
     3961 ggtcgctctt cgcgccgcgc ttcagctcgg tctgcggggc gccacgctcg taggactggt
     4021 tccactcgta gccgtcgcgc cagatcgtca gctccagctt ctccgagagc gcgttcacga
     4081 ccgacacgcc caccccgtgc agtccgcccg acaccttgta gccgccgccg tcgccgaact
     4141 tgccgccggc gtggaggacg gtcagcacga cctcggccgc ggggcgaccc tccttctcgt
     4201 gcatgtccac cgggatgccg cggccgttgt cggtgaccgt gacgctgttg tccgggtgga
     4261 tggcgatcga gacgtggtcg cactcgcccg cgagggcctc gtcgaccgag ttgtccatca
     4321 cctcgtagat caggtggtgc aggccgcgcg tgccggtcga cccgatgtac atgccggggc
     4381 gcttgcggac cgcttcgagg ccctcgagga cggtgatgtc cttggctccg taggaggaac
     4441 cttcctggga ggactctttc gccaaaactc tcttttcgtc ggggccgcgc ggagggggtg
     4501 cgcgagcgct ctctttacat gacgagaccc cgacgcccac cggtctcccg gcacggctcg
     4561 gggctccctc tacaacgggc tgaaggatag cagcggagct ggtcggaacc cggtcctgga
     4621 gtgttacaaa atgcccgcaa atcgggcaaa aacgtgaagt tatgtgaacg cttcgacccg
     4681 ggtggcgagc ggctcggggt gcggcgcggc gaaacggggg tcagcggcgg gcccggcgag
     4741 ggctcgccga gaccttcagc gagcgcactg gcggggggcc ttcggcggct ccgagggcgt
     4801 tgttgagacg gccgacgagg tcgcccccga gcagctcgat ctcctgcgcc cagaccgacg
     4861 acgtgcacgt gatcgtgacg accccgtcac gttcggcgac cggctccgcc tcccgcgcga
     4921 tcgccgcgcc cgcggcggcc ggccagcaac tctggacgcg cgcgagcgtg gtggccggcg
     4981 cgagctcatc gaccacgccg gcgagggcgc tggcaagcgg tcgcgggtcg cggcgcctca
     5041 tgcggcgcgc agcccgcgcg cgcccgcgcg ctccaccgac ccggtggtca cctcgaacag
     5101 cacgacgtcg cttgcgtccg ccccaggcac gtgctcggcg tcggtggtgg tgatcagggc
     5161 ctggccctcc gcgcgcacga ggtcggacag gcgcgcacgg cggccggcgt cgagctccga
     5221 catcacgtcg tcgagcagca tcagcggcgg cgtgccgcgg gccgccaggg cgtcgcgctc
     5281 ggcgaacagc agcgcgagca gtccgagccg ctgctggccc tgcgagccga gcgtgcgcag
     5341 cgcccgcccg ccatgcgtga gcgcgaggtc gtcgcggtgc gggccgtgcg tggtgaagcc
     5401 gcggtcgagg tcggccgcgc ggcgctcgac cagctcggcg cgtagcccgt ccgcgtcgcc
     5461 ggccttggag cgcggcgcgt agcgcagccc cgcccgctcg ggcagtccca gctcctcggc
     5521 gcgcgccgcg aacgccgggg ccaccagctc gaccgccgcc gcccggtcgg ccatgagctg
     5581 agcgccgtgg cgcgcgagct ccgcgtccca cacgtcgagc tgctccgcgg tggccgcgcc
     5641 ggcacgcacg cgggcgatga gcgcgttgcg ctgcgcgaga gcgcgcgtgt agcccgagcg
     5701 cgtagccacc cgcgcgggcc agagcgcgac cacgacctgg tcgaggtgcg cgcggcgcag
     5761 cgcgggcgca cccttcacga ggtcgagccg gtcaggcagg aacaccgcgg cgagcggccg
     5821 cggcggatcg acgccgcgct ggagcggcgc gccgtccacg cggatgcgct tctcgtcccc
     5881 cggcgcgatc cccacctcga gcgcgtgctc gccgtccggc gcctcgaccc gcgcctcgac
     5941 ccgcgccacc ttctgcccga tgcgcacgag ctcgcggtcg accgaggtcc ggcacgagcg
     6001 ccccgtgagc cccacgtaga gagcctccag caggttggtc ttgcccgcgc cgttcgggcc
     6061 ggcggccacg ttcagacccg gcccgagctc gacctccgcg cgctcgtagt tgcggaagtc
     6121 cctcagcgcc agcccggtga cgtgcacggc gaggacctac acgttcaggc gaatcggcat
     6181 gacgaggtag atgaacgacc cgccctcgtc gccggccacc gcctcgataa gccccggccg
     6241 cagcggactg atcagcttga agatcagctc gtcggcgtcc acgctctcga ggccctcacg
     6301 gaggaagtcc gggttgaagc cgatctcgag ctgctcgccc ttgaacggga tgggcagcga
     6361 ctcgctcgcc tcgccgatgt cgggcgtctg cgcggagatc tcgagctgcc cgtcgctgaa
     6421 cttcatacgc agcggcgcgt tcttctgcgc aaggaggctg atgcggcgca ccacctcgat
     6481 gatctccgcc ccgctcaccc gcagctcgtg ctcgaacgtc tccggcagga gctgcttgta
     6541 gttcgggaag cgcccctcga ccaagcgcga cgacagcgtg accggcccga cccggaagac
     6601 gacctggtgc tcgagcgacg cgatctcgat ctggccgtcg gcctccgtgc ccgccacgcg
     6661 gctcagctcc tgcagggtcc gcgccggcac gttcgcctcg agcgtgccgt ccagcgcctg
     6721 ttcgagcgcc gtctccttca cgctgagtcg gtaggagtcg gtggcgacca tccgcagctc
     6781 cttctcggcg gcggagacga ggacgccggt gaggtgcggg cgtgtctcgt cgcgcgaggc
     6841 cgcgcgggcg acccggccca cggtctcgac gaacgctcgc gccggcaccc gcaccacggc
     6901 cgcgtcgccg atctcgggca ggttggggaa ctcgtcggcg gggagggtgc ggaggtggaa
     6961 gcgcgccggg cccgagacca cctccacgtc cccctgcgcg gagcggtact cgagcgtgac
     7021 gtcgtccctc gggagcgagc gcactacgtc gagcagcaac ctggcgggga cgaccgcgac
     7081 gccgtcgcgg tcgggagcga cgtcgacgtt cacgcggacg cccagctcca tgtcggtggc
     7141 ctggagctcg acgcgtccgt cacccgcgcg gatcatcacg cctgcaagcg tctggatggc
     7201 gctgcgggtc gatgcgccac gggccgcgat gccgagcgcg gagagcagct gctccttgcc
     7261 gatcgacagc ttcatcgaac tgaaatgact gagttgattg cggtgggtgg tgttgacatg
     7321 tgcgtcgaag ttagagatcc cgctggcaga gcggagcgag gcgtgtcggg tggactgttg
     7381 attgtcacat caacaccggt cggcaggctg gccgccgagc acgtcgcgtg ccctgtcgac
     7441 agccgcacgg gtctcgtcgc ggctgccgat gtctccggcg atgcgcctgt gggcgtggat
     7501 gacggtcgag tggttgcgcc cgccgaagtg gcggccgatg gcggggaggc tctcctctgt
     7561 catctcacgc gccaggtaca tcgccacctg ccgggccagc gccaccttcg gcctgcgatc
     7621 gtgggcgatc agcgactcgg gcgtcacgtc gagcgcggtt gccgcggcgg cctggatctg
     7681 cccgagcgtg gtggatgccg cgggctcgga ggatgagtag agcgttccga gcacgcgcgc
     7741 ggccagctcc gggccgggct cctccccgcg gagcgaggcg taggcgacga cccggatcag
     7801 cgcgccctcc agtgcccgca cgctgctcgt cacgcgccgg gcgacctcgg cgagcgtcgc
     7861 cgcgggcacg ttcccgagcg cgtcgatgcg cgcgcgcttc tggaggatcg ccagccgcgc
     7921 ctcgaagtcg ggagcctcga gcgccgcgac cagcccgcac tcgaaccgct cccgcagccg
     7981 cgcctcgagc gcctctatgt cgcgcggcgc gcgatcgctc gtgatcacga gctggctgcc
     8041 cgcctcgtag agcgcgttga acgtatggaa gaactcctct tcggtgcgca gtttctcggc
     8101 gaggaactgg atgtcgtcca cgaggaggac gtcggcgtcg cggaagcgag cccggaacgc
     8161 gcgcgtgttc ccgctgcgca cggccttgac gaagtcgccc gtgaaggtgt cgacggtcgc
     8221 gtatcgcacg cgcagcccgt cgccgtagga ccgcacgtag ttgccgatcg cgtgcaggag
     8281 gtgcgtcttg cccaggccgg gcggaccgtg gatgaacagc gggttgtagg cctggccagg
     8341 ctgctcggcg accgcgaggg cggctccgtg accgagccgg ttcccgtcgc agatcacgaa
     8401 ctggtcgaag gtgtagcgag ggttgagtcc ggcggcggcg ggttcgccgt cctcggatgc
     8461 cgccgctgtg gcgcgggcgg ggcggggcgt ctcggccgcg ggcgcctgcc agcgttcgtc
     8521 gacgacctcc accgcccccg cgacgcccgc gcgcgaggcc gcgcgccgga tcacgccgag
     8581 gaagcgctcc tgcacccagc cgcgaatgtg gtcgggcgcg cgcacgaaga gcgtgccgct
     8641 ggagttgccg gcggcgccca gcggcgccag ccagatgtgg aaggtcgagt cggtgacctc
     8701 gccgcggagc tcgcgcacga ccgcctccca catctcggag aggtcgttcg gggacggcgg
     8761 ttcgaggtct ccgggcacgg gtcggcggca gccctccggg aggctgggaa gcggtctgta
     8821 ttcgatagga gagggcttgc gggaggtccc gaggccctcg gagcggccag tgtaacgcgc
     8881 cctcagcgac ccgtggagag cccgcgggcg gcttgccgca aatcgcggga aaagctccgg
     8941 acggctcgtt tcgagccgcc gtcgacaggc tgtgcacagc tcggcggacc tcacgcgggc
     9001 gctatgctct cgctcgattc cgcgcccgtt ttgggctgcc ctcatgaagc gcacctacca
     9061 gcccaagaag cgcaagcgtg cccgcaccca cgggttccgc gcgcgcatgc agactcgcgc
     9121 cggccggcac atgctcaagc gccggcgcga caagggccgg aggaagctca ccccataggg
     9181 atggcagcgg cgcacggcgg cggacggcgc cgcggcaggc gccagcgcct gtcccggagc
     9241 gccgagttcg accgggtgtt ccgcgaggga cgctcgcacg cgagcaggca gctcgtcctc
     9301 tacgcgttcc cgaacgccga gagcggcgag gagccgcgcc tgggggtctc cgtcgggcgc
     9361 aaggtgggcg gcgcggtgca gcgcaaccgg gtcaagcgcc tcctgcgcga ggcattcggc
     9421 gcgctctccg ccgagctgcc gggcgggcac gacttcgtgg tggtggcgcg ccccgatgcg
     9481 gccgctttcg ccgtcgatgg gggcgagcag gcagtggagg gtgcgctgcg cgagctgctc
     9541 gagcaggccg gcctggtgac cgccgcatga aggccctcgt gctcgctccc ctccacctct
     9601 acaggagggt tatctctcca ctgctgccgg ctcggtgccg ctactacccg acctgctcgc
     9661 agtacgcgat cgaggccgtg cgcgagtacg gagtgatccg cggctcgatc ctcgccgcat
     9721 ggcgggtcat gcgctgcaac ccgctcagcc atgggggatt cgaccacgtc cacgaccaga
     9781 ggctgttccg gcagtcgcag gcggtctgag ccatgtaccc ggtcctggcg aacgtccttc
     9841 aacctctgat caacgtcaac gaggcgatcc tcaacttctt ccacggggtc accaacagct
     9901 ggggggtcgc gatcatcggc ctgaccgtca cggtccggct cgcgatcctc ccgctcacct
     9961 tccgccaagt gaagggcatg cagcagatgc agcgcctcgc tccggagatg aagaagatcc
    10021 aggagcgctt caaggacgac cgtcagcgcc agcagcagga gctgatgaag ttctacaagg
    10081 agcacaactt caacccgctg tcgtcgtgcc tgccgctgct gctccagctc ccgttcttcc
    10141 tgtcgctgtt ctatctccag cgccagcagg agttcaagga cgaggtgcgc gagaccggca
    10201 aggagttcct gttcatcccg gacctcacca gcaaggccac gggcgcggtg ctcgtggtgt
    10261 tgatcgtgac gtacgtcgtc acgcagctcg ggtcgagcct cgtgacggcc gtgaacatcc
    10321 aggacaagaa ccagaagcgg ctgatgctcg cgctgccgtt cgtgttcacg gtcgtgatcg
    10381 tcaagttccc gacggggctg ctcgtctact ggatcacgac caacctgtgg accatcggtc
    10441 agcagctcgc ggtcagacgc ttcatgcccg cccccgcgcc ggtgaagtcg atcgacgaca
    10501 aggactcggg tgacgggggt ggaggaggag gcggaagcgg cggcaaggcc aagcggcgcc
    10561 aggggatgat ggagcgtgcg gcggcgcgga tggccggcga ggatgggaag tcgggcgacg
    10621 gcgacggcga ccgcgcgggc aaggccacgg gcaaggcgaa gggcgccggc ggaggcgggg
    10681 cggccaagag cagcggcgcg acggccaagg ggtccggcgg ctccggaggc gggaacggcg
    10741 ggccgcgcaa ggcgccaccg ccctcgccgc gcaagaagaa gaagcgctcc ggccgcaagc
    10801 gctagacctc gcgcgacgca ttgcggcaga atgccgcgcc atggttcccg gagaagacga
    10861 cgcgcccgcg cgcgcagatg gagaggcgcc gccggacgag ccgcaggagc gggtccgggc
    10921 gctggtcggc cgggtggtgc agggcctcgg cctgaccgcc agcgtggacg tggaggtcga
    10981 cgacgaggcg atccgcgcga ccgtcaacgg cgacgacctc ggcatcctga tcggcaagca
    11041 cgggagcacg atcgacgcgc tccagcacct ggcgctgcgg gtcgcctaca agggcggcgc
    11101 cgcggagcgg caggtggtcg tcgacgccgc gggctatcgc gagcggcgcg agaccacgct
    11161 gcagcgagcg gcggaccggg ccgtctcgga cgcgctcgac ttcgggcggc cggtcgagct
    11221 cgagccaatg acggcctccg agcggcgact cgtccacacg tacctgcgcg atcgcaacga
    11281 catccagacg cacagcgagg gcgacgagcc cgaccggcgg ctggtggtct cgccggtgcg
    11341 gtcgcacggc tcctgacgcg ctgccgtttc acgtgaagcg gtgcgcccga gcggttcgcg
    11401 ccggtgggcg caacgtttcc catgaaacgc ccctgtcgtg gtgagccgtc gcgacaaact
    11461 gattcgcgct ctggagcgcc acggcgtgga tccgacgcac gcggacacgc tcgagcggat
    11521 cctcgcggca ctcgacgccg agccggatcc gcataccagc gtctctcccg gcgaagccgt
    11581 ggatgtccac atcgccgaca gccttgcggg gctcgcggtc ccggcgctgc gatcggccca
    11641 cacgatcgcc gacctgggcg ccggggcggg cttccccggc ctcgtcctgg cgaccgtcct
    11701 cgcgcacgca cgcgtggacc tgatcgaatc cgcatcacgc aagaccgccg tgatcgaccg
    11761 tctcgcagcg gcgggcggtc tccagaacgc ccgggcggtc accgcgcgcg ccgaggactg
    11821 ggccgccgcc gagggccgcg aggcgtacga cacggtgacc gcccgggcgg tggcgccgct
    11881 ggccgtgctg gtggagtacg ccgcgccgct actgcggccc ggcggggtgc tcgtcgcctg
    11941 gaagggcgcg cgcgaggacc aaggggaggc cgccggcgca gccgctgccg cactggtcgg
    12001 cctgcgtccg gccgacgtcc tcccagcgac cccctacccg gcgagccgca atcgccacct
    12061 gaacgtcttc gaaaaggttg cgccgacccc cccgcgcttc ccccgccggg ccgggatggc
    12121 ggcgaaacgc ccgctgggcg gggcggggcg ctgaggctta ggccgcgccg gccgccgacc
    12181 gccgaccgcc ggccgcgtgg ccgcgtgggc cgttcacgcc gcgatcggga tgccgaggtg
    12241 cggtgccgcg cttagtcctg cccgcgcaac cgggcgctta ccgcgccagg gatgccgcgc
    12301 agctgccgac ggcgcgcacc tggcctggga caccctgtct ctgccgggga tggcgcgcag
    12361 ctggcccgac tgcgcatcca gccgggccgc cctgtcttct ggccggagcc ggcgcgcacc
    12421 tccactttca ctagaacgaa gttctagaac atggttctag tggtgggagg tgtgcgtgcg
    12481 acctctccgc tctgcggcgc ctggccggcc tcggcctccc agccccgccc caccgcccca
    12541 catcacaccc cgcccccacc gcggcccggc aggaatctgg acgcacccgt cgaagcacgc
    12601 cacaaaccgc tccaacaacc gcttccgacc gcaatccctc gtaaggtcgc cgccgatggg
    12661 aatcgtctac gccattgcaa atcagaaggg cggggtcggg aagaccacga ccgccgtcaa
    12721 cctcgccgcc tgcgtcgccg acgccggcta cgagaccctg ctcgtcgacc tcgacccgca
    12781 gtgcaacgcc accgtgggcc tcggcgcgcg caaggacctc gagccgaacg tctacacgtg
    12841 cctggccggc gacgccccgc tccacgacgc ggtgatccgc accgacgtcc ccggactgtc
    12901 gctcgcgccc gcggcacccg acctcgccgg cgccagcgtg gagctcccgc gcgagccggg
    12961 ctccgagacc cgcctgcgcg acgcgctcga cggcttccgc gaccgctacc tcttcaccat
    13021 cctcgactgc ccgccgtcgc tcggcccgct cacggtcaac gccctggtgg ccgccgatcg
    13081 cgtgatcgtg ccggtccaga ccgagtactt cgcgctcgag ggcctggccg gcctgctcga
    13141 cacgctctcg ctgatccagc gcgagatgaa cccgcggctg tcggtctcgg gcctcgtact
    13201 gacgatgcac gacgggcgca cgcgcctcgc ccaggacgtc gagcgcgagc tgcgcgagca
    13261 cttccccgag ctggtcttcg attcggtgat cccgcgcaac gtccgggtcg gtgaggcgcc
    13321 gagcttcggc gtgccggtca cgcgccacga cccggcttgc tccggctccg tggcctacct
    13381 gaagctcgcc cgggaggtgg cggcacgtgg ctgagcctgc acgtatacct gagcccgagg
    13441 cgaccaggac gggcgagccc gaggcgatcg ccgcggggcg ccagcgcggc atgggccgtg
    13501 gcctcgccgc gatcctctcg accggcccgc gccccgaggt cgggctgcgt gagctcgcgg
    13561 tcgagctgat ccgcccgaac ccggctcagc cacgcagcga gttcgacgag gacgccctcc
    13621 tggcgctgtc gcgctcgatc gccgcgcgcg gagtgctcca gccgatcgtg gtgcgtccgc
    13681 tgcccggcgg cacctacgag ctggtcgccg gcgagcgccg cctgcgcgcg gcacgcctgg
    13741 ccggtctcgg cacgatcccc gcggtggtcc gcgagaccgc cgatggcgac cggctcgagc
    13801 tggcgctgat cgagaacatg gcccgcgagg acctcaaccc gatcgaggag gcgcgcgcgc
    13861 tcgccacact ggtggacgac ctgggcctca gcaaggagga gctcggccgc cgcgtgggcc
    13921 gcagccgccc ggcgatctcg aacatgatcc gaatgctgga gctgccggac gaggcgctgg
    13981 cgatggtcga gcgcggcgag ctctccgcgg gccacggccg cgcgctgctc ctgtgccgcg
    14041 gccacgatga gcgcctgcgg ctcgcccgcc gcgcccgcga ggagtcgtgg tcggtccgcg
    14101 tcaccgagga ccgcgcgcgc gaggccgagg acggcggccg cgctcgcgag ccgcgcgggc
    14161 ccgtggtgat ccaccccgac ctcgccgact cactcgcggc ggcggaggat gccctgtccg
    14221 cggcgctcgg ccgcgaggtg cgcgtcaagc cgcgcgcagg cggctatcgc gtcgagttcg
    14281 acctcgacca cccgcgcgag ggcgtcgacc tcgcggagcg gatcctgcgc cgcaacgggg
    14341 cctagaggca gcgctagact cgcgctctcc gggcgattag ctcagtcggt tagagcgctt
    14401 ctctgataag gaagaggtcc caggttcgag tcctggatcg cccatccgcc gtcgatcatc
    14461 gtggcggcac gagcgacgcc accaggtccg tgaccgcgtc gtccacggcc tccgggtcgg
    14521 cgagcttctc cagcgccagg cccctccaca gcgccagtag caccacggcc agcgtcgcgc
    14581 tcgggatcgg cagctcggcg ccgttcgcct cggcgcgctc gtcgatcatg cccgccaacg
    14641 tcgcgcggat cccgcgcatc cgcgacgcga agtgccggcg cagcttcggc ttgcgcaccg
    14701 cgtaggccca gaactcgatg aagaccagta gctgctccgg gtccctcgtc aacccgtcga
    14761 gccaatcccg gaacgcctgc gcgggaacgg gtgcgtcgcg ggccgcctcg agctggtcgg
    14821 tgtaccagcg caggtggccg tcgaagacgg cgaggaaaac gtcgtccttg cccttgaagt
    14881 tcgagtagag cgcgcccacc gagtagcccg cctcgtccgc gatcgcgtcg accgtcgcgg
    14941 cgtggaagcc gcgccgcgcg acgaccttcg tggcggcgtc gaccagccgc gcgtgcgtcg
    15001 ccgccctccg ctcggtcacg gcccgcttcg cggtcacccg cgtgacctta ctcacaccga
    15061 cggcatcctc ctgtcagcat agtgagcgct cgctatcaat tagaggagag agcgacggca
    15121 tggcacaggg agcggaggta cgcgagggaa cggtgagggc gaacgggatc gagttcgcca
    15181 cgatcgaggc gggggagggg ccgctggtcc tgctgctgca cggcttcccg gacaacccgt
    15241 ggacctacga ggcgcagatc ggcgagctgg ccgcgaacgg ctaccgcgcg gtcgcgcccc
    15301 acatgcgcgg ctacccaccg accggctccg cgcccgacgg ccgctacgac tccgaggcgc
    15361 tggcgggcga catcgccggc ctggtgcggg ccctgggtga cggccccgcc tacgtgatcg
    15421 gcaacgactg gggtgcgatc gccacctacg cggccatgtc gctgcacccc gagacgatca
    15481 gccgcgcggt ggtcatcaac gtcggtcacc cctccacctt cctgaccacg ctcctgcacc
    15541 cccaccaggt gcaccacatc ttccacttct ggttcttcca gctcgggccg atcgcgacgg
    15601 gcgcggtacg cgccaacgac ctcgcgttcg tcgactacct ctgggactac tggagcgcgc
    15661 cgggtcacga ctacgccgac cacctcgcgc gcgtgaagcg gacgctcgag cccgagggcg
    15721 ccgtcgaggc cgccacgtcc tactactccg gcctgctgaa catgccgacc gcgcaacccg
    15781 acgcggcggc tcggatgcag ggacgcatca cggtgccgac gctgtcgatc ttcggcgacc
    15841 acgacccggt gcgcgagctc tcgcgcgacg agcacgagca cttcgacgcc gactaccgcc
    15901 tcgagatcgt ggagggcgcc gggcacttcg tgcaccgcga gcgccccgac caagtcaacc
    15961 gcctcgtcct cgactggctc ggagccgccc gatgacctac gacgactaca agtccagcac
    16021 aaggaccacg accactcccc acggcgagtt cgcctacctc gacgcgggtg agggcccgcc
    16081 ggcgctgttc gtccacgggc tgttcatgag cggctacttc tgggcgccgg tgataggcgc
    16141 ggtgaaggac gtgcggcgct gcatcgcgta caacctgccc gaccacggcg ggaccaccgt
    16201 cgcggctgag caggacctca gcctcgacgc gaacgcggag atgctcgagg cgttctgcga
    16261 ctcgctcggg ctcgaccgct tcgacctcgt cgccaacgac accggcggcg cgattgcgca
    16321 ggcgctggcg gtgcggatcc ccgaccgggt gaagacgctc acgctgacca actgcgaggc
    16381 acgcgactgg atgccgtcga agcacgacct cggtcagctc gtgaagagcc tcgcggagca
    16441 gggccagctg gcgcaggtgc tcgcggccaa ctacgaggac cgcgacgccg cgcgctccac
    16501 cgcgttcgcc gcgaccttcc agtggcccga ccgcccctcg gacgacgacg tgcgcggcct
    16561 gatggagccg catcaaacga cactggatgc ggcgaagcgg ctcgagcggt tcgccgcggc
    16621 gatcgagccc aacgagctga tggcgatcga gccgcggctg cgggagctga cggtcccgtc
    16681 gctcgccgtg tggggcaccg gcgatccgat cttcccgctt gagctggccc actggctgcg
    16741 cgacaccatc cccagcctcg acgaggtcgt ggagatcgag ggtggcctgc tgttctggcc
    16801 gtgggagcgt cccgacgagc tcgctccgca cctccgtcgc cactggacct agcgccgggc
    16861 gaaccggcgc aaagccgcgc ccagcagttt ttcgcgcaac cccggcgggt accacaaggc
    16921 gtaccccata cgaacgtgct gcaccgcgcc ggggggcgcc cgtcaaggac ggcggttcag
    16981 ggcgaagaag gaggagacca tggcgtggaa taagcgcttt gaccgccaga acggcgccgc
    17041 gggtgaggat ccgctgcacg gaaacgtcgc gacgcgcacc cgggacccgc tcccgcagca
    17101 ggccccgccg ccggaccccg aggacgggat cgctccgcgc cgcgagcgca cgcatccggt
    17161 cgacgaccgg ccgcacacgg gccgccgcgc ggcggcgacg gccgccacga ccacgaccgg
    17221 tcacggcgcg cgtgaccgct tcggcgggct caactggggc gcggccttct tcggctggct
    17281 cacggcgctc ggcatggccg cgatcctcac cgcgctgctc agcgcggcgg gcgccgcgat
    17341 cggcctgacc gagggctcgg cggccggctc ggccgaggac aacgccgaga ccgtcggaat
    17401 cgtcggcggc gtgctgctgc tggccgtgct cgggatcgcg tacttctgcg gcggctacgt
    17461 cgccggccgg atgtcgcgat tcgacggcaa gcgccagggc ttcggcgtgt gggcgatcgg
    17521 gttcctgatc accatcgcga tcgccgtggt cgcggctgtc gccgggtccg agtggaacgt
    17581 gttcaaccag ctcaacctcc cgcgcatccc gatcgacgag ggcgacctcg cgacgggcgg
    17641 tgcgatcgcg ctggcgctcg gcctgctcac gacgctgatc gcgtcgatcc tgggtggcgc
    17701 caagggccgt cactaccacg accgcgtcga ccgcgcggac ggccgcgact ggtagtcgca
    17761 ccgcacagga cgtaccttca cggaggccgg gcgctactag tgcccggcct tcgtgtttga
    17821 tacgcccgtg aacacctcat cgcgcatcgc actcacggcc tcggccctca ccctcgtcct
    17881 gccggccacg gccggcgcct cgaccatcga ggtctccgcc tcggcgggtc ccgcggccag
    17941 cgagttcctc cagtaccgcg cctcgaaggg cgaggccaac cgcgtccgcg ttctgttcgg
    18001 caagaactcc gtggtgctgg tggacaagtc cacgaagcgc atcaccgtca agcgcgacca
    18061 ggggttcggc cgctgccgtg cgaccagcag gcaccgcgtg gtctgccctg acatgccgct
    18121 gttcgcgagc ctgcgcgaca gcaaggaccg cttcagcgtg gcccccggcg accgcgggga
    18181 tgcaccgacc tcgaccaacc cgcgctcgta cgccgcggac tacgaggaca ccgagggcgc
    18241 ggtcacggtc acgctcttcg tagacgccgg cagcggcgac gactccgtca cggggtcgaa
    18301 gtacgacgac acgatcctcc ccggccccgg cagggacacc gtcgatggcc gcgacggtcc
    18361 cgacgcgatc gactccgagc ccgacggcgt aatcgactcg ctcgacggcg gtggcgacat
    18421 cgactcgatc tcgttcttca gctcggcgcc cgtgagcgtg gacctcgcgg ccggagccgg
    18481 cggcccggcg ggcgagctcg accggctggc gggcttcgag cgcgtgcacg gcggctcggg
    18541 cgacgacgac atccgcggca ccgagacgac cgacgccctc tacggcgaga gcgggcgcga
    18601 tcacctggac gggcgcggtg gcaacgacat ggtcgtgggc gacgcccccg acgtcacgaa
    18661 cgggagcccg aacgagctca tcggcggcga cggcgacgac gtcctcgaca cgcgcgccga
    18721 gaagccggcc cccacctcga ccgtcgcgtg cggcgccggg aacgacagcc acatcggagg
    18781 cgtcgatgcg ctgctcgatc cctcgtgcga gtcggcgctg ctgcgcgtgg gcttcggcac
    18841 ggtcaccgat ccggagaccg aggaggtgct ctacgacgac ccgatgaagg tgacgccggt
    18901 ggccaagacc tccgacagca tcacgttcga ggtcccgtgc ccgaagcacc gcaacggcgc
    18961 gtgcaccggc aagctcgagc tgccggacta cggcagcgcc gacttctcgc tctcacccgg
    19021 cgagcggcgc agcgtgaccg tccccctcaa cgcggcgggc caggcggccg tggcgggcag
    19081 ctcgcccgtg gcggtgcggc tgagcatgga cctcagcccc gtgaaccagg gccagttcgt
    19141 ggcgcggacg tccctcggct tccaggccga cctctagagg gcaggcggcg ggccgcccgc
    19201 gcggcgcgct cagtcggtcg ggttccggct gcccacgccg gtcatgaact cgcgcacgcg
    19261 ccccggcccg ctcgactccg gggcgagcgg cggcgtgtcg gcgccggagc cgtcgcgcag
    19321 cccgttcagg aggtccatga gcgcctcgcc ggagctgtgc ttcgcctccc agccgagcac
    19381 gtcgcgcgcg cgcgtcgtgt ccatgatcgg cacgttcagc gcgaggtcga cccagccggg
    19441 cggcgtgggc tgcgcgtgcg cctgccagct cgcgtcggcg gccgcgcgca ccgcggccgg
    19501 cgggatcgag accttcttcg cgccgaacag ctcggcgagc atgtcgggat cgatcaccgg
    19561 gtcggcggcg atgttgaacg cgccgcgcac gtcgcgcacg accgcgagcc ggtacgcctc
    19621 gccggcgtcg tacgagtgca ccgcctggac cacgagaccc tcgatgtcgg ggaccacggg
    19681 gatgagctgc gggcgcatga gcgagccggg caggagcggc ccgatgaagt agcggcggat
    19741 ctcggcggcg gcctcgcgct tgaacaccag ccccttgcgc aggcgcacga cgcgcatgcc
    19801 tgggaagtcc gtttcgatct ggtcgagctg gcgctcggtc gcggccttgt ggcgcgagta
    19861 gaacgaggtg tcgatcccgt cggtcggcca gctctcgtcc acgccgcggt ccttcggccc
    19921 gggcgagtag gcaccgatcg acgacgcgta gacgagcgcg cccacgcccg cctcgccggc
    19981 cgcgcggaac acgcgcccgc tgccgtccac gttcacggcg tgcagctgct cttcgtcgcg
    20041 cgagggctgg atcagccacg cgaggtggat cacggcgtcg gcgccctgga agtgcggcac
    20101 gagatcgtcg ctgacgatgt cggcctccac gaagctggtc ttcggccact gcatccccgg
    20161 cagccggcgt gcgatcccga ggatctcggt tacctccggc tcgtccacga gcgagcgcag
    20221 cacgctcgtc ccgacgttgc ccgtggcacc cactactacg acgcgcatct cagcctccca
    20281 gcggtgtctc gccgagccgc tcgagcagct cgggaatcga ttcgaacacg gcggccgcgc
    20341 cgccctcgcg cagctcgtcc tcggagaacc cgccggtgat cagcgcgatg gtctcgacgc
    20401 ccgcggccgc ggcggccttg cagtcccacg gggtgtcgcc gagcatcacg gcgtcgccgg
    20461 gctcggcgcc cgccttctcg agcgcggcct tcaccaggtc cggctcgggc ttggtggcct
    20521 cgacgtcgtc ggaagtcgtc cagtcgtcgg ccagctcgcg cgcgtcgagc aggtcgaggt
    20581 agtgctcgac ctcgttcccc ttggcggagc tggcgagcac caccgtccgg ccgtcgtcct
    20641 tcagccgctt gatcaggtcg cgcgcgcccg cgagcggctc gacctcctcg atcagcgcca
    20701 ggtacagcgc cttctccgcg gtgcggatgt cgtcgccctt ctcctcgtcg gtctgcttgt
    20761 cggtgagcga ctcgacgagc tggtcgccgc ccatcccgag gtgccggtgg atcctccaga
    20821 tcggcaggat cacgtcgtgc tggcggaacg cgcggtacca ggcgacggcg tgctggtagt
    20881 tggtgtcgac gagggtgccg tcgatgtcga ggatcgcggt ggaggccatc actcgttccc
    20941 ggccgcgcgc gccagccgcg cggcctcggc ggcgccgccc tcgaacggat cgccgtgccc
    21001 gacggcgacg gtgcccgcgt cgagggcccc gatcttgtcg agcgagcgca gcgcctgctc
    21061 ggtgtcgttg gtcgctccct tggcgacgag gcgcgggccg gtcgaccccg tgtacgggtc
    21121 gaaggtcacg atcgcgtcgc cggcgatcac caggtcgcgc tcggggaagt ggaatgcgac
    21181 gtggcccttc gtgtggccgg gggtgaagac cgcgtgcggg cgcccgggca cgtcgaccgg
    21241 gccctcgccc gcgtacgtgc gcacctcctt gaccggccgg gcgaagaacg cgccgcgcct
    21301 gaggaagtcg agcccgacct tgcgcaggcc gcggtccttg aggtagacca ccggcagccg
    21361 ctcggtgcga aagaaggcgg ggtgcttgct gagcgagacg tcgtcctcgt gcacccacac
    21421 cgggatgccg agctcgcggc ggatgcgctc ggcgaatccc acgtggtcgt agtgcgcgtg
    21481 tgtgagcacc agcgccttga cgtcggacag ggtgcggccg agctgcctga ccgcgtcctc
    21541 gaggagcttc cacccggcgg gtgtgccggc gtcgaccacg gttagcgagt cgccgtcctc
    21601 gacgaggtac cagttcacgt agccgtcctg gatccggtgg atcccctcgg ccatgtcgcg
    21661 tcgcagcatc cctacccgcc tacccggcgg gcgggcgcgc tacgcaaccc gctcgatcgc
    21721 gggcgagctc tcctcctcgg cgatcaggtc gagcacggcc tcctccaccg gcccgtggag
    21781 cgagtcgagg tcgttcgtgt cggcgacctc ctggagtacg ccgccgatcg taaggccgga
    21841 gtcgaagcgg tcgaacacgc gcgggtcgat gtaggaggcg cggcagaccg ccggcgtgtt
    21901 gccgaggtag tgcgcgacct ccttgacggc gcgcaccttg gcgcggttcc gtgcggtctt
    21961 cgacatcgtc gcggccgggc ccgagaccgc gagcgcgacg gacgcgagca cggtcgcgtt
    22021 ccacgtgcgg aagtccttcg ccgagaagtc gccgccggtg acctccttca cgtacacgtt
    22081 gatgtcggcg gacttgatgt cgcgccagcg cgtgccctgc ttgtacgcga gcagctccgg
    22141 cccgacgccg ccgcgacggc gcttcagcgc cgcgacgatc tcgtacacct ggtcgtccac
    22201 gatcgactgc acgcgccgct ggccgctctt ggcggggtag tcgaaggtga tcgtgccgtc
    22261 acccacccgc acgtgctcct tgcggatcgt ggcgagcccg tagctctcgt tctccaccgc
    22321 gtagtcctct ccgccgaccc ggaagaaccc gcggtcgagc agccggacgg agcaggcaag
    22381 cacctgcggg cggctgagcc aatcggagtc gaggtcgtgc gccacgcgct cgcgcatccc
    22441 cggcagggcg cgcgcgaagc cgatcatctc gtcgaacttg agctggtcct gacgctcgcg
    22501 ccacttcggg tggtagaggt actgctttcg cccgcgcgca tcgacgccgg tggcctggag
    22561 gtggcccatc gggtacgggc agacccacac gtcctgccac gcgggtggaa tcgccagctc
    22621 gttgatgcgg ccgagcacct ccgggtcgtc gacccggtcg ccgtcctcgt ccacgaacac
    22681 gaagccgcgc ccgcgccggc ggcgcgtgat gccgggcgac gagcagtcga cccgccgcag
    22741 gcgcgccatc agcccgacac cgccgcgagc agctcgggat gcgtggccgc cgtgacgcgc
    22801 gaacgcatgt cgaggtcgag cgacccgctc aggtcctcgc cgccagcgtc cgggaacgac
    22861 accacgccgc cggcctcgcg tacgacgagc tgcgccgcgg cggcgtccac cgaccggcag
    22921 ctccggaggc tcagcatcgc gtcgaagcgc atgcctccca catagcagag cgacagcgcg
    22981 atcgaaccga tcatccgcag tcgccgcgcg cccgtcgccg cgagcagatc ggccgctccg
    23041 gccacgaggt ccggtcgcgc ggactcgacc ccgagcacct ccagctcacg cgtcgaacgc
    23101 gtcacgtcga gccgctcgtc gcccagccag gcgccgcccc cgcggcgcgc ccaccactcc
    23161 tcggcgccgt cgaacgtcgc cacgtatccg aagtccacgt cggccatcga cgggcccgac
    23221 gcaaccgcga tcgagaccgc gtgccacggc atcgcgcgct tggcgttcag cgagccgtcg
    23281 atcgggtcga gcaccaccac gagcggcccg ccgccgccca cctcgacctc gccgcgctcc
    23341 tcggtcacca cacgcgcgcc gacgccggac tcctcgagca cctcgagcac cgcgtcctcg
    23401 gccgcgcggt cgatcgcgag cgccatgtcg ccgccctcgc cgcgccaggt ctcgcgcgcg
    23461 cgagcggccg ggtcgtcgaa cggcgcgacg gcggcgcgcg cggaggccgc ggcccgccgg
    23521 cagagcccga gccagtcctc cgagggcgag tccggggaac ccatgggcgg gcgtatccta
    23581 ggcgcgatgc cgcagcggac cgccgaactc agcgctgcgc agcgcgaggc ggtcaaccat
    23641 cgcggcacgc cgctcctgat ccgcggcggc gccggcaccg gcaagacgcg cacgctgctg
    23701 gagcgcttcg gcgcactggt gcgcgagggc gagccggccg actccgtgct gctgctcgcg
    23761 ttctcgaacg cggcggccgc cgaggcgcgc gaacggctcg agacctcgat ccagacgccg
    23821 tgggaggagc tgtgggcgtg caccttccgg tcgttctgcg cgcgcctgct gcgcgaggag
    23881 gcgctcgagg ccggcatcga cccgttcttc gtgccggtga cccccgcgga ccggctcgcg
    23941 ctcctgctcg accgcatcga cgagctgcac ctgcgccgtc acgagattcg cggcaacccg
    24001 gccccgttgc tcggcggctt cgtcacgcgc atcgaccgcc tgaaggagga gatgatccgc
    24061 ccggcggact accgcgcgca cgcggagggg ctggcggagc tggcgcgcga cgacgccgac
    24121 cgcgcgaacg cggcgcgcga gctcgagctg gccgccgtct acgagcgcca cgaccagctc
    24181 ctcgcccagc gcggcgcgct cgacgccggc gacatcgtgc tgcgcgcgtt cgacctgctg
    24241 ctcgagcgcc cgcacgtgcg ccgcgggctc ggcgagcgct tccgccacat gctcgtggac
    24301 gagtaccagg acgtgaactt cgcgcaggcc gccgtgctgc ggctgctcgc cgccgagcag
    24361 cgcgacgtgg ccgtcgccgg cgaccccgac gcggcgatcc accgcctgcg cggcgcgggc
    24421 gcgaagaacc tcgacgactt cgagcgcgag cagcccgacg ccctggtcat ccggctcgac
    24481 cgcagccagc gctccgggcg ccggatcgtg accgccgcgc aggccgtggg tggcccggac
    24541 acgcggctca ccgcacgcgc gggcggcgag gtgaccttct ggcactgccg ctccgaccgc
    24601 gcgcaggcgc agcgcgccgc gctcgaggcc gagcagcgga tcaccggcgg cgtgccgccc
    24661 gaccaggtct gcgtgctcgt gcgcgaggtg gcgggtgagg gcgcgctagt gggcgcggcg
    24721 ctcgaggagc gcggcatccc gttccgcctc gccggcgcgg cggcgtactt ccagcgctcc
    24781 gaggtgcgtg acgtgatcgc gtggctgcgg ctgctcgccg acccgggcga ctccggcgcg
    24841 gtcgtgcgcg cgctctcgcg cccgccgatc gagctgcgcc aggtggacat cgcgcgcctc
    24901 acgcagctcg cccggcgccg caagctcgac atggtctccg gcatcgaggc ggcctgcgag
    24961 gcgcccggcc tctcacccga gggccgcgac cgcgcgctcg cgttcctgcg cgtgtaccgc
    25021 gccgcgtcgc gcgcgttcga ggagatgtcg cccgaccgct tcgtgcaccg gctgatcgag
    25081 cggatcggcc tgcgccgcca gcaggtcttc gccgcgaagg ccgacaccgt cgagcggctc
    25141 gtgaacatcg cgaagctcgc cgacctcgcc gtcgagtacg tgcgccgcga gccgggctcg
    25201 tcgtcgcgcg acttcgcccg ctacctcgcc gccgtcgccg aggcggggct gcccgaggag
    25261 gaagccgcgc cgggcaacca cgccgggagc gcggtccagg tgatgtcgat gcgcgacgcc
    25321 aagggcacgg agttcgacac cgtgctcgtg ctcggcctgt cggcggcggc gatgccgggc
    25381 tcgcggcgcg agggcggcca tcgcatcccg gccgcattgc tgaaggagga cctgcccgag
    25441 cccgacccgc gcgaggagca cgaggccgcg atgcggcgcc tgctgcacgt ggcggtgacc
    25501 cgcgcgcggc ggcggctcgt gctgtcgtgg cccgagggcg accgcggccc aggcgcgcgg
    25561 ccgtcgccgt acttcgagga cgcgcgcgcg gccgtggagg cagaggtgga ggtggtcgag
    25621 gaggagctgt tcggccccgc cgagggcctg cactcgacct tccggatgat gcgcgacgag
    25681 ctgctcgaca gcgtcgcgca cgtgggcggc cggctcggcg agatgcgcct cgacacctac
    25741 ctcgaggtct cgcgctcggt ggcgcgctac ctggagctgc tgaagatcgc ggcgctgatc
    25801 gagcgcggca aggccggcca gagcgtgaac gacgcgctcg ccgaggtgaa cgagctgctg
    25861 ctccagggcg cgagccccga gcaggccgag ctgttccggc tctcggcgct cgacgactac
    25921 ctgctcgaca ccgccgggag cgacgagcgc cgccgccgcg aggcgatcgt ggccacgccc
    25981 gacgacacgc tcgagccgtt catcccgcgc cgcggcgagg ggctgatgct gtcggcgtcg
    26041 gatatcgaga cctaccggct ctgcccgctc aagtacaagt tcgcgcgcgt cttccgcatc
    26101 ccgcaggagc cgagcatcaa ccagcgcttc gggatcgtcg tgcaccaggt gctcgagcgc
    26161 ttccacgcga ccgacggcgc ctcgctcgaa acgctcatgc agctgttcga gtcggcctgg
    26221 cggcggtcgg gcttcggcga ctccaacgac gacctccagt tccgcgagcg cgcgctggcc
    26281 gcgttgcggc gctactggga gctcgaccgc cgccgcgagt cggagccggt gtcgttcgag
    26341 cgcagcttct cgttccacat cggaccgcac ctgctgcgcg ggcgcgtgga tcgcgtcgat
    26401 cgcctgcccg acggccgcca cgagctgatc gactacaaga ccggcaaggc caagaccgag
    26461 caggacctgc gcgaggacgt gcagctgtcg atctaccaga tgggcgcgcg cgaggcctgg
    26521 gggctcgaga ccgcggcgca gagctactac tacgtgctcg agaacgagaa ggtgccggtc
    26581 gcgcactccg aggaggagct cgagcgcgtg cgtgggacgg tcaccgagat cgcggaccgg
    26641 atcatggccc accgcttcga gccgaagccg tcgttcgagc tgtgctcgtt ctgcgactac
    26701 aggatcgtct gcccggcggc cgagaagtga ggcccggcgg cctcgccgag ctggcgctcg
    26761 atccggcgcg cgatgtcgcg cgtcgcctcg tgtaggaagg tgatcttccc ggccgcggtc
    26821 tcgtagccgc ggacgaagac gaccagcgtg ccggccccgc actcgaagcc gggcttgagg
    26881 gggtgctcct ggagccatac cacgaagctc ggggagaaca gctcgcgcag cgcgacctcg
    26941 tcctggccgt ccgcgcggtg caggtcgtag atgtcgttga agcgcccgct ctcgagcttc
    27001 acgcggtggc gcggggagcc gaccgcgagc cagtcgtcct ccagcttgat cgcgctgagc
    27061 cgcgggcgca ggtagacgcc gcgcaggcgt ccgagcggcc cgccgatctc ggccgcgcag
    27121 accgtgaagc ggtgcggcac ccaatcgtgc cgctcgacgt acgcctcgtg gtcctcgatc
    27181 gcaatctcgt acgtgtagtg gccgagcacg cacgtcggcc ccccaagtcg tccgaacagc
    27241 ggccccgaga gggtgtgctc gaagtggcgg cgctcgccgg cgccgagcag tggcgtcacc
    27301 ggcgggtagc cgccgccgga cgtgtactgg agaccgagcg acggagcctg catgaacagc
    27361 gcctcaccgg cctccttggg aggctcgcgc cggccgacca ggtcggcgac catcgcggcg
    27421 tccggcacgt actcgcggac gagccgtcgg aggttcacgc gaacgagacg gccgggacca
    27481 ggcgctcggc gggtgagtcg agcacgaaga acgcgcgcgc cgggaagctc gagccggcca
    27541 gcagcacgtt cgggaagacc tggacgcgcg tgttgtaggt ctggacgttc gagttgtaga
    27601 tgcggcgcga tgcctggatc tcgtcctcga gctgggcgag gtggctctgg agcccgcgga
    27661 agttcgcggc ggcctggagg tcggggtact gctcggcgag cgcgcggacg tcgccgagcg
    27721 cggcggtcag cgcgccctcg gtgcgggcca cgtcggcggg gtcgctggcg gccatcgcgg
    27781 caccgcgcgc acgcgtcacc tcggtgagcg tctcgcgctc gtgcgcggcg tagcccttga
    27841 cggtctcgac gaggttcggc acgaggtcgt ggcggcgctt gagctgcacg tcgatgccgc
    27901 tccaggcctc ctcgaccttg ttgcgcgcgc tgacgaacga gttccaggtg acgacgatcc
    27961 agacgatcag caggatcagc ccgccgccga tggcgtaaaa gaccgtgtcg ctcacctggc
    28021 ctgaatcggc cgtggcgcgt cggcgcttga gttacgaacc ggtctctgga ccgcgctggc
    28081 gcaggaagcg ctggaagtcg cgtgcgatcg cgtccgcgga cggtatgcgc ggggagggca
    28141 tctcctcgac cgcctcgtcc atcgtctgct cgagccgctc gacaaagctc ttcacgtccg
    28201 ggtcgctggc cacggccgcg ctcacctggc gctcgtagtc ctcggccgac tcctcgagct
    28261 cgcgcgcctc gacggccacg ccagcggcgc cctcgaagct gcggacgagc gcgagcgccg
    28321 ccttcgggtt gggcgcggcc gcgacgtagt gcggcaccga ggcccacagc gacaccgagg
    28381 ggatgcccgc ggcggcgcac gcgtggtgga ccacgccgac gatgccggtc ggcccctcgt
    28441 aggtcgagcg ctcgaagccg agcttctcga ccagcgcctg gtccgacgcg atgccggtca
    28501 tcggcaccgg gcgggagtgg ggcacgtcgg cgagcagcgc gccgagggtg atcacgctct
    28561 cggcgccgag ctcgcgcgcc atgtcgatca ggacctgcgt gaatgttccc cagcgcagcg
    28621 acggctcgac gccctcgaac aggatcaggt cgtgctcggc gccggcgacc tgcgcggcgt
    28681 ggaacgagtt ctccggccac tcgatcaccc gcgtgaggcc ctcctcgagg tgcacgttcg
    28741 ggcgcacggc ggtgaagtcg tagaactcct cggggtcgat ccgggcgaac tcggtcgcct
    28801 cgaagctgtc cacgaggtag tcgagcgcgg cggtcgccgc ctcgccggcg tcgttccagc
    28861 ccttgaacgc caccaccagc gccggccggc gcagcacggg ccgctcgctc cactcgacat
    28921 ggtccatgta gatgacggta cccgaacgcg atgttctgcc ccacggcttc aggcgcgcgg
    28981 ccgcggcgcc gatgctcgcc agatggagcc ctcgttccgg cggaccctgt acgccctgat
    29041 cgggtgcggc gtgctgatgc gcctcgcctg ggtggcgcag accggcgggc tgccgttcga
    29101 catgcagtcg ttccgcgccg tctcaggcgc gctgctcgat cagccgctgc gcctctacgg
    29161 ggagctcaac ccacccggca tgtaccgctg gccgtatccg cccggctact tcccgtgggt
    29221 gctcgcgagc cacggcctcg actcgagcgg gctgctcggc ttccgcgcgg cgatcgtgct
    29281 cccggccatc gccgccgacg cggccctcgc gtggatcgtg caggccgcgc tgggccgcgg
    29341 cggcgcatcg gagcgcaccc ggctcggtgc cgccgcgctg gtcgcgctcg gcccgtcgtt
    29401 cgcgctgatc tccggctacc acggtcagat cgacgcggtc gcgatcctcc ctgccgtcgc
    29461 cgccctgctc gtctgggagc gcggcggctc gcggcgcgcc ctcgcggcgg ggctgctgat
    29521 cgggctcggc gcgtcggtca agtcggcgcc cggactggtg ctgctggcgc tgctgcccac
    29581 cgtggcctcg cggcgcgagg cggccacgct gatcggcgcg gcggtggcgg taccgctcgc
    29641 cgcgctcgcg ccgttcctcg ccgccgacgc gtcgggtgtg gtgcgcgctc tcggctacgc
    29701 cggcatcccg ggggaggggg gcctgacgat gatcgtcgac cccgacctcg cgcgctactg
    29761 ggtgcagaac gcgaaggacg ttcgcccgaa tggcctctcc agcttcctga gccagcacgg
    29821 cagcctgttc accggcgcag cgctcctggc cgccgccggc ctgctcgtgc gccaccgcgt
    29881 ggagccgcgc cgggccgcac tgatcctgtg gctcaccttc tacgtcctgg gcaccggctt
    29941 cgcgttccag tacgtgatct gggggctgcc gttcctgatc ctcgccgggc gcctgcgctg
    30001 ggcgttcgcg ctccaggccg cgctgctcgt tcccactctc atctactaca ccgggccgtt
    30061 cccggccctc gatgtgctcg tgccggtgta cttcgtgctg atggcggcgg tctggctcgg
    30121 cctggccgcc gcgctcgccg tgacggcgct cgaccggcgc cgcgcccccg tgactgcgta
    30181 gagtccgcgg ccgtggcagg ccggctcgca gccgcggtcc tcgcgtccgc ggcgtcgctc
    30241 acgctctcgg cgtgcggtgg cggcagcggc ggcgaaccgg agcgcgtggt cgtcgcgctc
    30301 gacttcaccc cgaacgccgt gcacgcgccg atctacgccg cacgcgacga gggcatcgag
    30361 gtccgcaccc cgggctccgc gccggacgcg ctgaagctcg tcgcggccgg ccgggcggac
    30421 atcggcgtcc tcgacatcca cgatctcgcg ctggcggtcg agaaggggag cgacgtggtc
    30481 gggatcgccg cgcttgtgca gcgtccgctc gcggcgatca tcgcgcgcgg gaacgtgcgg
    30541 cgcccgcgcg acctcgaggg tcgtcgcgtg ggtgtatccg ggctgccgtc ggatcccgcg
    30601 gtactgcgcg ctgtgctcga gcacgacggc ggccgcctcg gcgcggttga ccaggtgacg
    30661 atcggcttcg ccgccgtgcc gaacctcgtg cagcgcaagg tggacgccgt accggcgttc
    30721 tggaacgcgg agggagtcac gctgcgccgt cgcggcgtcg acatccgcga gttccgcgtc
    30781 gacgactatg gcgcgccgcg ctatcccgag gtggtgctct tcaccagtcg ccgcgcgctg
    30841 cgcgagcacc gcggcaagat cgagcacgcc ctgcgtgcga tccgtgcggg cttcgactcc
    30901 gtggagcagc ggccgggtgc cgccgcacac gcgatcgccc gcctctcggg cgccgacgtg
    30961 gagctcgtgc gcgcacagct cgacgcggtc gcaccggcgc tgcgcccgcc gctgcggctg
    31021 gaccgtccgc tgctcgagcg ctgggccgac ttcgacgctc gcttcggcat cgtccaccgc
    31081 cggccggacg tgggccggct gttcgacttc gggatcgcac cttgacggcg gtcgccccct
    31141 ggccgccgcc cgcggtcccc gcgctcgaag agggcgacgt gcaggtctgg tcgctggacc
    31201 tcgacgccga gcccgacgca agcctgctcg acgccggcga gcgcgagcgc gcgggtcggt
    31261 tcgtgagcga cgagctgacc cgccgctgga cgaacgctcg cgccgggctg cgaggggtgc
    31321 tggccgctta ctgcggggcc gacgcgacgc agctgcgcat cgagtcagcg ccgtgcgtgc
    31381 actgcgccga gccccacggc aagccgttcc tcgccgagcc gtcggggcac gacctgcgtt
    31441 tcaacctctc gcactcgggc acggtggggc tggtcgcggt ggcgcgcggg cgcgaggtcg
    31501 gcgtggacgt cgaggccatc cgcccggggc gccgcgccga cggcatcgcg agccgctggt
    31561 tcaccccgga ggagtccgac cacctgcgcg gactcgggga ggccgaggcg gagctcgcat
    31621 tctaccgcct ctgggcgcgg aaggaggcct acctcaaggc cacggccgcg ggcgtgcccg
    31681 gcggcctcga ttcgttcgac gcgctgcacc tcgagctcgc gtccggcgcc gagccggggt
    31741 gggagttcgc cgacctggac gtgggcgccg gatacgcggc ggcgctggcg gtcgcgccgc
    31801 cgggttacag acccggcggg tgacgcgcga cgaacccgcc gaacgagcgc ggcgccgcct
    31861 gccagcgcga gccgcgcgcg ccgctgcggc cgctcgtgtc gaagcgcagc ccgcccacga
    31921 ccatgaacat gtgaccgggg ttcgagtaga tcgtgatcca cttcccgcgg ccctcctcgc
    31981 cccacttcat gaagttcccc gaggccatcg gagccttcag caggccgccc gccgccagcg
    32041 cgtagctgac cgaaccggag cagtcgtagc cggagtcctg gaaggagccg tggccgccgc
    32101 cccagatgta cgggaagtcc gcgatcgcgt tgccgccggc gatcaccgcg cgcacggcct
    32161 gcggcgcgtt cgacgggacg cgcgccagcc cgcccttcgt cagcgtcacg ctgccggccg
    32221 gtccgccgcc gccctcgcgc atctcgcgca gcatctcgcg cacctcctcg tcggaggggg
    32281 cgccggggct cacgccgtcg tcggacgagg cgcccgccac gtgcggcgcc ttggccttcg
    32341 gcccgtcctg agcggcctcg gggtccgggt ggctcagcga ctcctcgggg tcgcgcacgt
    32401 gggcgaccgg cgtcggcgtg ccggggccgt cgctgagcga gaccacctcg ccgcggccgg
    32461 ggttcgcggg cggcgccggg gcggtcgcgt cgtcgcgcgc gggctgtgcg gccagcgccg
    32521 cggcggcgcc ggcggcgagc agcacgacca cgagcagctt cgcgaacagc ggcgcccgca
    32581 gcggcagacg gacgagacgt gacatcccct cgtctatcgg cgcgggccgc ggagcctcaa
    32641 ccccgtcgcc gcgaccgccc tctggcctac gatccgtcac ggtcgcgcac ccgactgcgg
    32701 agggacccct tgcacgcacg catcacggcc acggcggctg ccgctctcgc cacaggcgcg
    32761 ctcgcgctcg ccggctgcgg cagcgattcc gggccggagc tgaaggtggg ggcatgcacc
    32821 aacgcggacc ctacagaggc actctcgttc gacgtgaagg ctgtcgactg cggggacgac
    32881 gaggccaagt ccaaggtcgt caagcaggtc gagaagtcca accagtgcga cgtcgcgtcg
    32941 gtgtccgaca gcgccaacga caaggtctac tgcaccgagc cgtacccgcc gggctcggca
    33001 cccacgaagc cggaggtcgg cgcctgcacg acggcggacc ccaaccagct tgtgagcgtg
    33061 aacatccgac tcgtcaactg cgacgacaag gaggcgaagt cgaagatcat cgagcgcgcg
    33121 gcgcaggagg acgagtgcaa gaagggctcg atccgcggct cgggcggcga gatcttctgc
    33181 atcgcgcccg tgcgctaggc cgcgggctcg aagtcgtagc gcgaggcgat ctcggccatc
    33241 agggcctggt cgggcgggcc gccggacgtc gccgcggcca cgacctcgcg gagatagcgc
    33301 tcgaacccgc cgggcgccat caggttgagg aaccgcaccg gctcgtcgcc ggggttggag
    33361 aaggtgtgca cggtgcccgg gggcaccagc gcgaaggtcc ccgcgtcgag cccgagggtc
    33421 tcctcgccta gccgcacggt cagcgtcccc tcgagcacgt agaagctgtc cacgaacgtg
    33481 cggtgcacgt gcgcgaccgg tccggggaag ccgggcgcgg cggtcgtctc gctgagcgag
    33541 aagtgccccg cgtcgtcgtc gccggtggcc ttgaacacgg cgctcgtggg accgaatctc
    33601 agcggttcgc cctcgcccgc gtcgagcacc cgcgcgtccg acgccggccg gccgccgtcc
    33661 gccggggggt cgtgctggtc gaagcgctcc cgcgcggcct ggtcgccgtc gcggcccgcg
    33721 cgcaggtagt cgtcgaagcc catcgacggc gcatgcaagt tcagcgcacg cacgggggcg
    33781 ccttcgtcgg cgttgccgac cgtgtgcacc acgttcggcg ggatccgcac gaagccgccc
    33841 gggtgcagcg gtcgctcctc gccgccgagc cggacggtca gcgttccctc gagcacgtag
    33901 aacgcgtcgc tgtggtgatg gtgcacgtgc ggcggcgggc cgggggtgcc ggggctgagc
    33961 cgcacctcgg agacgaccac ctgctcgtgc gcccccttga tgacgatccc gccggcgggg
    34021 tggtcgacga tgatctcgcc ctcgccgggc cgggcgacga ccgcgtccac gcggctagcg
    34081 gatcgcggcg ccggcgaccg aggcgatgcg ccagggctgg tcgtcggacc cgctgagcgc
    34141 catcgtccag cgctcggtga acgtcgtctc ggaggtgtcg ctgccggcca cgacggccgc
    34201 cgtgtcgcgg ttctgcacgt agcgcgtgcc cgtcacgtcg agctcgagtg tcatcgtcgg
    34261 cggctgcgcg gcggcgtcca gcgccgcgat gtgcaggcgc gtgatgcgcg ggccgcggac
    34321 caccaggcgc gtggtccggc ttgggtcgcc cgggtgcagc aggtcgccga gcgcggcggg
    34381 ctcggcgatc gcctcgagct cgcggtcctc gccgtcgacc gcctcggccc aggccgcgac
    34441 cgcccggcgc gcggccacct cgagcacgtc gggcccgaag cgcgcgtcgg ccagcgacag
    34501 gtcgagcgcc gccgcgcgcg cctcaccgtc gaagtcgagg tccgcgacct ccgccaccgt
    34561 gaagccctcc gcgaccctgt tcgccaccgc gccctcgacg agcgactcgt cgcgcagccg
    34621 ctggtcgtcg gcccacggcg tggcgacgac atccgacttc aggtggtgca tcccctcgtc
    34681 gtcctgctcg atcgagagca gcatccagcc gtcgtcggcg gtgcggcaga gcgtccacca
    34741 ctcggccagc tcccgcgtct cgtcggactc gccgtccgac atcatccggc cgccgccctt
    34801 catctgcacg tagtcctcga gcgtggcctc cacgcgcacg accacgcggt cctcggtgtc
    34861 gtcctcgcgg ttcacgagcc ccacgtacag gcacttcggg ccgctcttca cgttcacgac
    34921 gttgtgccag cccttgcggt cgaagtcgtc gagccgcttg ctccactcct tcatcaggtc
    34981 cgagccgacc atcgcgttca gccgctcgcg gtccttcgcc gaccaggcca cctggatctg
    35041 cttgaacagc tcggccgcgt cgcgcttgac agcgtcggcg gcgaggtagg cgtcgtcctg
    35101 cgcggcctcg gccgaggccg tcgtcacgcg ctcgtcgcgc ttcaggatcc gccgccgctc
    35161 gagggcgttg acgatcatcg cgccgacgaa gatcacggcg atgatcaccg cgaagatgag
    35221 gaagtcgagg aagctgccct cgccgctgcc cgagccgcct ccgccgctgt agccgccccc
    35281 gcctccgccg ccaccaccac cgccgaagcc cgagctgccg cctccggcgg cggccagcgc
    35341 gtcggcgggc gcgaggagca ggagggcgag cgggacgagt gctgcggtgc gggttctcat
    35401 gccgccacgg taaccgcagg cgggctcttc tcgccaccgc tcagcggcgg gcgggtctgc
    35461 accatccggg ccccgtggcg
//
LOCUS       DASVPL010000169         4683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_216141, whole genome shotgun sequence.
ACCESSION   DASVPL010000169 DASVPL010000000
VERSION     DASVPL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216141"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VF517_08855"
     CDS             complement(<1..359)
                     /locus_tag="VF517_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053090.1"
                     /translation="MADSAVARAKAALLAAGGEPATAAAEAFLRRTALRAGVAVVYHG
                     LAERPGDPARDVVPAHGAELFERQLAYLAARYRLVPASRLAAAERMRRRRDPFPLAVT
                     FDDDLPSHAGLALTLLER"
     gene            complement(352..1962)
                     /locus_tag="VF517_08860"
     CDS             complement(352..1962)
                     /locus_tag="VF517_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053091.1"
                     /translation="MAREDDREELGRRIERLARWHGGSATWTWAGSNVAAGVVRYDDR
                     GEDLGTPLVFGEPLPPGIGPLLDAEPEALRALDGAGAALEIEAARAGLVAYAGGPGTL
                     YGASCGPIDAWSTHAVAAAYLALGRARVDPTAIPEQLAAEFVGGERSLIAGARPLAQA
                     VRVVADAEGARLTDYWPLGERWAPVPEDEAADHAEKHLLESLARRAGAMGGEPHASLT
                     AGLDSRVAAVALREAGIAPRGFTWGEPDWDDVRGGAEVARRLGLEHELVGIEWLGDED
                     AMREVDRQVRWTEGGVPVGFARLAWPAGMRCFVTGAGGEVGRAFYYAGAEEADGDWPE
                     DLLVALVTGRLPDARLEALQELTATLNLWVSATVAAGFPGFRALDVVYGEQRVRRWLR
                     GMLPRLAAPMIPAFATPEVARALASLPLADRMSDGFHRGFLDRHAPDLVKPVVEPPPK
                     PPRRRLPWRRTAPASVLGERWEGHPGFRDWIAEGVFGSPLLVEPLGERWASRTRDRFL
                     ARDGDAERMALSAAGPVALDRALEELDG"
     gene            complement(1999..2976)
                     /locus_tag="VF517_08865"
     CDS             complement(1999..2976)
                     /locus_tag="VF517_08865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8053092.1"
                     /translation="MAGDGQPHVAVVVLSWNGRDDTLECLRSLGELRWERLSVVVVDN
                     GSTDGTLEAVRGGFPHVHAIRSEENVGFAEGNNLGIRAAIEAGADYVLILNNDTVVDP
                     GVVEVLVAEAQRRPDAGALCPLIRYADPPDRIWYAGARFDPRAVHNGRHTGYGERDTG
                     QYAAVREIGRATGAAMLVPREVIERTGCFDGRLFIQVEDVEWSLRMRDLGYRVLFVPD
                     AVVFHRVAMTSGGERAPVTAYYEMRNMLEVCARHAPLRGPARLWREYGSLAVHLFHAR
                     LASQPIANARAVIDGWRDFRAGRLGPRPQGRDPRRAGPAQPARTHAARG"
     gene            3029..>4683
                     /locus_tag="VF517_08870"
     CDS             3029..>4683
                     /locus_tag="VF517_08870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8053093.1"
                     /translation="MTDVTVVIPTRDRWDVLTRTLAALRRQELGGVEAEVVVVDNGSR
                     TPAPELPGARVVVERTPGAAAARNRGIAEARGRIVLFVGDDCLPADEHLVRGHVEAHA
                     GAEPRAVTGRIEWDPAIERTAVMDWLERSGHMVDMERLRREPAGPETFYTGNVSVPRQ
                     ALLEAAGFDQRFSGYGFEDLELALRLADRGVHFEHRPDLGVLHSHRYALEHSLARMEA
                     VGRGGRLLHTIHDHRRPLPGPPAGRARLASGRLLSVLPPVWLRAAHLAAFARGYRAAP
                     LDDHPSARGYGAVPRFSDEPRPAVSVVVPFLGSRGEGETLLAALGELRLGREGDEVVV
                     VDNGGTLPDGAPGVVRALAERSSYYARNAGADGARNDWLLFLDADTLPPASLVDDYFR
                     EPIDERCGAVAGGVVAARGQRALVARYAASRRYLSQAAHMRDVHRPYGITANLLVRRA
                     AWEGVGGFFEGLRSGGDQDLCWRIQDAGWSLAYREPAAVEHRHRERVLPLMRQMARYS
                     AAIAWMARRAPGSSPRPRVLRRLTRTAAGVLVWGVTGRGERAA"
BASE COUNT          560 a         1834 c         1742 g          547 t
ORIGIN      
        1 cgctcgagca gggtcagcgc gaggccggcg tgggagggga ggtcgtcgtc gaacgtgacg
       61 gcgagcggga acggatcgcg gcgacggcgc atccgctccg cggccgcgag ccgcgacgcc
      121 ggcaccaacc ggtagcgcgc cgccaggtac gcgagctggc gctcgaacag ctccgctccg
      181 tgcgcgggga ccacgtcgcg cgcggggtcg cccggccgct ccgccagccc gtggtagacc
      241 accgccacgc ccgcccgcag ggcggtccgc cgcaagaacg cctcggccgc ggccgtggcg
      301 ggctcgccgc cggcggccag cagcgcggcc tttgcacgcg ccaccgccga gtcagccatc
      361 gagctcctcg agcgccctgt cgagcgccac aggaccggcg gcgctcagcg ccatgcgctc
      421 ggcgtcaccg tcgcgggcca ggaaccgatc gcgcgtgcgg ctcgcccagc gctccccgag
      481 cggctccacg agcaggggcg agccgaacac gccctcggcg atccagtcgc ggaaaccggg
      541 gtgcccttcc cagcgctcgc cgagtaccga cgccggcgcc gtgcgccgcc acgggagccg
      601 ccggcgcggc ggcttcggcg ggggctccac gaccggcttc accaggtccg gagcgtggcg
      661 atcgaggaag ccgcggtgga agccgtccga catccggtcc gccagcggca gcgacgcgag
      721 cgcccgggcc acctcggggg tggcgaacgc ggggatcatc ggcgcggcca ggcgcggcag
      781 cattccacgc agccagcgcc ggacccgttg ctcgccgtag accacgtcga gcgccctgaa
      841 gccagggaag ccagccgcga cggtcgcgct cacccatagg ttcagcgtcg cggtgagctc
      901 ctggagcgcc tcgagccgcg cgtccggcag ccggccggtg acaagcgcga cgagtaggtc
      961 ctcgggccag tcgccgtccg cctcctcggc accggcgtag tagaacgcgc gccccacctc
     1021 gccgccggcc ccggtcacga agcagcgcat ccccgccggc cacgcgagcc gcgcgaaccc
     1081 gaccggcacg ccgccctcgg tccagcgcac ctggcgatcg acctcccgca tcgcgtcctc
     1141 gtcgccgagc cactcgatcc cgaccagctc gtgctcgagc ccgagccggc gcgcgacctc
     1201 ggccccgccg cgcacgtcgt cccagtccgg ctcgccccag gtgaacccgc gcggcgcgat
     1261 gcccgcctcg cgcagcgcca ccgctgccac gcggctgtcg agcccggccg tcaggctcgc
     1321 gtgtggctcg ccgcccatcg cgccggcccg acgcgcgagg ctctcgagga gatgcttctc
     1381 ggcgtggtct gcggcctcgt cctcgggcac gggcgcccag cgctcgccca gcggccagta
     1441 gtcggtgagc cgcgcgcctt cggcgtccgc caccacgcgc accgcctggg ccagtggccg
     1501 cgcgcccgcg atcaggctgc gctcgccgcc gacgaactcg gccgcgagct gctcggggat
     1561 cgcggtcgga tccacccgag cgcgaccgag cgccaggtac gcggccgcca cggcgtgggt
     1621 gctccacgcg tcgatcgggc cgcacgacgc accgtagagc gtgccgggcc ctcccgcgta
     1681 cgcgacgagc cccgcgcgcg ccgcctcgat ctcgagcgcg gccccggccc cgtcgagcgc
     1741 ccgcagcgcc tcgggctcag cgtcgagcag cggcccgatc cccggaggta gcggctcgcc
     1801 gaagacgagc ggcgtgccga ggtcctcgcc gcggtcgtcg tagcgcacca cgcccgcggc
     1861 gacgttcgag cccgcccacg tccacgtcgc cgagccgccg tgccagcgcg ccaggcgctc
     1921 gatccgccgg cccagctcct cgcgatcgtc ctcgcgtgcc actcgcgcga ggaacaggca
     1981 gcgatcgagc gacaaggctc agccgcgcgc cgcgtgggtg cgcgcgggct gcgccggccc
     2041 cgcgcggcgc ggatcgcggc cctgcggccg cggccccagc cgcccggcgc ggaagtcgcg
     2101 ccagccgtcg atcacggcgc gcgcgttcgc gatgggctgc gacgccagcc gcgcgtggaa
     2161 caggtgcacc gcgaggctcc cgtactcgcg ccagagccgc gccggcccgc gcagcggcgc
     2221 gtggcgcgcg cacacctcga gcatgttgcg catctcgtag tacgcggtca cgggcgcgcg
     2281 ctcgccgccg ctggtcatcg cgacccggtg gaagacaacg gcgtccggga cgaacagcac
     2341 gcggtagccg aggtccctca tccgcagcga ccactccacg tcctcgacct ggatgaacag
     2401 ccgcccgtcg aagcacccgg tgcgctcgat cacctcgcgc ggcaccagca tcgccgcccc
     2461 ggtggcgcga ccgatctcgc gcaccgcggc gtactgcccc gtgtcgcgct ccccgtagcc
     2521 ggtgtggcgg ccgttgtgga ccgcgcgcgg atcgaaccgg gcacccgcgt accagatgcg
     2581 gtcgggcggg tccgcgtagc ggatcagcgg gcagagcgcg cccgcgtccg gccgccgctg
     2641 cgcctcggcc acgagcacct cgaccacgcc cgggtcgacc accgtgtcgt tgttgaggat
     2701 cagcacgtag tcggcgccgg cctcgatcgc cgcgcggatg ccgaggttgt tgccctcggc
     2761 gaagccgacg ttctcctcgc tgcggatcgc gtgcacgtgc gggaagccgc cgcggaccgc
     2821 ctcgagcgtg ccgtcggtcg agccgttgtc gaccacgacc acgctcaggc gctcccagcg
     2881 cagctcgcct agcgagcgca ggcactcgag cgtgtcgtcg cggccgttcc agctcagcac
     2941 gaccaccgcg acgtgaggtt gtccgtcccc ggccaccgcg cggcagagta gccgagcggc
     3001 ccggcgccac gggtagcctg cgggtcgcat gacggacgtc accgtggtga tcccgacgcg
     3061 cgaccgctgg gacgtcctca cccggacgct ggccgccctg cggcgccagg agctcggcgg
     3121 tgtcgaggcc gaggtcgtgg tggtcgacaa cggctcgcgc acgccggcgc cggagctgcc
     3181 cggcgcgcgg gtggtggtcg agcgcacccc cggcgccgcc gccgcgcgca accgggggat
     3241 cgccgaggcg cgcgggcgga tcgtcctgtt cgtgggcgac gactgcctgc ccgcggacga
     3301 gcacctggtg cgcggccacg tcgaggcgca cgccggggcg gagccgcggg cggtcacggg
     3361 gcgcatcgag tgggatccgg cgatcgagcg cacggcggtc atggactggc tcgaacgcag
     3421 cggtcacatg gtcgacatgg agcgcctgcg gcgcgagccc gccgggcccg agaccttcta
     3481 cacgggcaac gtgtcggtgc cgcgccaggc gctgctcgag gccgccgggt tcgaccagcg
     3541 cttctccggc tacgggttcg aggacctcga gctggcgctg cggctggccg accgcggcgt
     3601 gcacttcgag caccgccccg atctgggcgt gctccactcg caccgctacg cgctcgagca
     3661 ctcgctggcg cgcatggagg ccgtcgggcg cggcgggcgc ctgctgcaca cgatccacga
     3721 ccaccgccgg ccgctgccgg gcccgcccgc gggacgcgcg cggctcgcgt ccgggcggct
     3781 gctgtcggtg ctcccgccgg tgtggctgcg cgcggctcat ctcgcggcgt tcgcgcgtgg
     3841 gtaccgcgcg gctccgctgg acgaccaccc gtctgcgcgt gggtacgggg cggttccgcg
     3901 gttctccgac gagccgcgac cggcggtcag cgtggtggtg ccgttcctcg ggtcgcgggg
     3961 tgagggcgag acgctgctcg ctgctcttgg ggaactgcgg ctggggaggg agggggatga
     4021 ggtcgtggtg gtcgacaacg gggggaccct gccggacggg gcgcccggcg tggtgcgggc
     4081 cttggccgag cgctcgtcct actacgcccg caacgccggc gcggatgggg cccgcaacga
     4141 ctggctcctc ttcctcgacg ccgacacgct cccgcccgcg agcctcgtcg acgactactt
     4201 ccgcgagccg atcgacgaac gctgcggcgc ggtcgcgggc ggcgtcgtgg ccgcgcgcgg
     4261 acagcgcgcg ctcgtggcac gctacgccgc ctcgcgccgc tatctgagcc aggccgcgca
     4321 catgcgcgac gtgcaccggc cgtacgggat cacagcgaac ctgctcgtgc gccgcgcggc
     4381 gtgggagggg gtcggcggct tcttcgaggg actgcgctcg ggcggcgacc aggacctctg
     4441 ctggcggatc caggacgccg gctggtcact cgcctaccgc gagcctgccg ccgtggagca
     4501 ccgccaccgc gagcgcgtgc tgcccctgat gcgccagatg gcccgctaca gcgccgccat
     4561 cgcctggatg gcgcggcgcg cgcccggctc ctcgccgcgg ccgcgggtgc tgcgccggct
     4621 cacccgcacc gcggccggcg tgctcgtgtg gggggtgacc ggccgcgggg agcgagcggc
     4681 gtt
//
LOCUS       DASVPL010000170         9715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_257249, whole genome shotgun sequence.
ACCESSION   DASVPL010000170 DASVPL010000000
VERSION     DASVPL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257249"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VF517_08875"
     CDS             <1..791
                     /locus_tag="VF517_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8053094.1"
                     /translation="GRTDYTLRTTPKVVGGLTDRCLDRAMALYGEVCDNLVKVTTLEA
                     AELTKLLENVFRSVNIAMVNELKVLFTRLGIDVWRVIEAAKTKPFGFQAFYPGPGLGG
                     HCLPVDPFYLDWKAREYDTPTEFIELAGEVNQNMPYFCVEKITRALNDHSKPLRGSKI
                     AIFGVSYKAGVGDLRESPALKIIRLLLARGAEIAYSDEFVPALPEFGLEHADVDEALA
                     GADAAVIVTAHPGLDVASVVESSPLVVDFRGVTRGIDAANLVRL"
     gene            complement(816..1799)
                     /locus_tag="VF517_08880"
     CDS             complement(816..1799)
                     /locus_tag="VF517_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8053095.1"
                     /translation="MPPDEGIHALPIPTPFAVGRVNLYLIEDDPLTLVDAGPNSGRSL
                     DELESQLRALGHRLEDIGLILVTHQHIDHIGLVSIVARRSGAEVAAIDRLVPFMENYS
                     EATAAQDVFASELMLRHGISPDVVGALQSVSLAFRAWGAGAEVTRVLHDGDHVELRDR
                     RLEVLHRPGHSPTDTVFHDPDRRVLLAADHLIAHISSNPLVTPLWDGKAPDVRERPQA
                     LVAYLDSLERTAAMDLDLVLPGHGEPIAEHRKLIELRLKLHAKRAGKLHRLIEERPRT
                     AYELAQALWGNVAVTQAYLTLSEVLGHTDILENEGRVREVEEDGVVRFEAA"
     gene            complement(1789..3162)
                     /locus_tag="VF517_08885"
     CDS             complement(1789..3162)
                     /locus_tag="VF517_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX8053096.1"
                     /translation="MIDSAIFKAYDVRGLHPEQIDADVAYRVGRAFVRVIADGEGRPP
                     GEMRLGLGRDMRLSAPELAARYADGMRDEGADVLDVGMVGTEMLYFAVGSRELDGGLM
                     CTASHNPKGYTGAKLVKRGAIALSGDSGIGELRRIVEEGEPGPPAEARGDYAEEDVAD
                     EFRAAAAKFVDLDAIRPMKVVLDGGNGMAGPMVGPLLDRLPLDQAATYWTPDGEFPDH
                     EPNPLLPENRTFIVKKVLSEGAELGIAWDGDADRCFFIDDNGQFVDGDFLTALLAESI
                     IAKEPGAAILYDVRASRAVADVVERAGGSAHVNRVGHAFFKTRMRDEGAAFGGEVSGH
                     YYFRDFYCADSGTIPALLILELLSQKGKRMSELLADFRSRYFISGEINSEVADGEARM
                     RELEDRYSDAEITHLDGVSIDYEDWHFNVRPSNTEPLLRLNLESLVSPEHMEQKRDEV
                     LALIRAA"
     gene            complement(3159..3647)
                     /locus_tag="VF517_08890"
     CDS             complement(3159..3647)
                     /locus_tag="VF517_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX8053097.1"
                     /translation="MPDERLWAPWRLEYIKGPKADECIFCTKPQAGDDRGTYIVRRGE
                     HCFTMLNAFPYNNGHVMVAPYRHVPSIEELEGPALLELMTMAQDSLGALRRAYGPEGF
                     NLGINQGKVAGAGVEHHVHLHVVPRWGADTNFMPVVSGTRVLPQSLSDSYDALTEAYA
                     AA"
     gene            complement(3661..4602)
                     /locus_tag="VF517_08895"
     CDS             complement(3661..4602)
                     /locus_tag="VF517_08895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8053098.1"
                     /translation="MATCAACASTVLIPHLEVADARAEDLIPTTDRFGAALGDIVRCP
                     ACSHMQLDRFPSEAELGEAYGEAESEDYVDEEAGQRATARTALERIERYAPRPPGGAR
                     APGAAAEAGGSAPALLDLGCWVGFLLAEARERGWDTLGVEPSEFASSYARERLGLDVV
                     RADLFDADLGGRRFHAIAMGDVIEHLPRPDDALDRMAGLLEPGGILYLALPNAGSRLA
                     RRMGARWWSVIPTHVQYFTRPSLLTLLRRRGWEPLWTGTAPKAFTVRYYLERIGGYSP
                     PLANALVAAAAATGVADRMWAPDFRDRMAVIARAPGQ"
     gene            4628..5629
                     /locus_tag="VF517_08900"
     CDS             4628..5629
                     /locus_tag="VF517_08900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8053099.1"
                     /translation="MPERDVRGGRVGLVSVVAPMFDEEETVEVFYDRVRRALDGVRFE
                     LVLVDDASRDRTPELLDRLASSDPRVKVLHLSRNFGHQAAITAGLEHARGEAVVMLDG
                     DLQDPPEVIPQMLDRWRAGADVVYGVRESRAGESRFKLVTASSFYRVFARLTKLDLRK
                     NSGDFRLLDRRALDAVLAMRERNRFLRGMTVWVGFVQTAVPYKRDARYAGETKYTLRK
                     MVRFSLDAVASFSSFPLQIATFLGFLCAGFAFLLLPLVVVARLLEIYDAGVPSLLFVV
                     LLLGGIQLITVGIIGEYVARIYDEVKGRPLYLVRDARNVTAEQPDGAPSEPQRVPAP"
     gene            5632..6996
                     /locus_tag="VF517_08905"
     CDS             5632..6996
                     /locus_tag="VF517_08905"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8053100.1"
                     /translation="MRVAVLGAGVAGLTAAYRLTQRGHVVDVYERWPGLGGQAATLDA
                     GDGHLIERYYHHWFTSDRHIVELCEELGIGDDVEWRPSSVGMFADGVAHPFTTPVDLL
                     RFTPMSLPARLRMGLAVVWIQRRHPTVEGFHDVTARSWVVRAMGRQAWDKVWGPLLHG
                     KFGARADEIAMAWLWGKLTTRRQIKGEEARKELLGYPRTSFEGLFRRLRDEIEGGGGR
                     VLIDRPAASLARSDEGFTVAPGAVDSFRRGHDPRSFAVDGAPERYDGVIATVPSDVFE
                     QLLDPDLATAVGEPYLERVRGTEYQAALCLVLELDRRFGRFYWLNVADASLPFIGLIE
                     HTNFVEPERYGGRRFLYVANYLEQGDPLLALSPEELLEHYTPGLRRVNPEFSAGWVRR
                     SWLFREPAAQPIVPVGYAHRIPPLRTPVPGLVLANTTQIYPEDRGTNYSVRLGGEAVE
                     ALLG"
     gene            complement(6993..7928)
                     /locus_tag="VF517_08910"
     CDS             complement(6993..7928)
                     /locus_tag="VF517_08910"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8053101.1"
                     /translation="MPAFTLTGFAVLRAVAEHGTFTAAAEALGYAQSAISRQVAAMED
                     AAGAPLFERGRRGVRLTAAGARLLTHANAALDHVDAATREMTSLAATTRGRLRVGAFP
                     TAVAALVPRALTRHASEEPLVEILLREGTTPTQLRRLRAGALDLAVLGSPLGGELDAP
                     DLTLTRLLDDPMLLAVARSHPFASRRTVEVVEFAGERWISGSTNTADALLGAWPRLDW
                     KPDVAYVVRDWTAKLGLVAEGLGIAVVPALAAVAVRSDVALVKVRAEPPVSRTVAVAL
                     PASRERPPLVAGFIDQLERVAAALRVEIDERLGPS"
     gene            8027..8440
                     /locus_tag="VF517_08915"
     CDS             8027..8440
                     /locus_tag="VF517_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8053102.1"
                     /translation="MRAVFEGLAAGDGTRLVEAMSDDFSWTIAGVATHWAGTWRGKQA
                     VREELLAPLFAQFADRYVNTPLSFTAEEDRVVVESRGRVTTKAGARYDNHYCYVCRFD
                     DDNRLASIVEYADTALMEAALHPPESRARTPIRTS"
     gene            complement(8395..>9715)
                     /locus_tag="VF517_08920"
     CDS             complement(8395..>9715)
                     /locus_tag="VF517_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8053103.1"
                     /translation="LQAALDRGAALPEPLRRGMPASEARAAMEALPAAERDGLAERLA
                     AAGGGAGGEAAGLPGAAAGGDSARLAPADIARIAAAGHEIGFHTRGHRVLTELPDDEL
                     AHELADGRDELAAAAGAPVELIAYPFGVADARVAAAARSAGYGAGFTVAPAAVTPASD
                     PLLLPRVDAPYEPPARLAWHVARALLGALTAGAESGSPTADARRALLGESAGRALRRA
                     RTAPRVGSLDLGDLGTTTPVSPGFGFERGTPVDRHYIDRFLERHAADIRGRVLEISED
                     RYTRTFGGDRVERVDVLHVEEGNPQATIVGDLTDAPQIPDASFDCVVCTQTLLLIWDV
                     PAAIATIRRILKPGGTALVTIPGITRVCREEAESWGDYWRFTRQSAQRLFEEGFGEVS
                     VETFGNVLTATAQLHGIASEELDPAARDVHDPDYEVLIGVLARDSGG"
BASE COUNT         1308 a         3348 c         3664 g         1395 t
ORIGIN      
        1 cgggccgcac cgactacacg ctgcgcacca ccccgaaggt ggtcggcggg ctcaccgaca
       61 ggtgcctcga ccgggcgatg gcgctctacg gcgaggtctg cgacaacctc gtcaaggtca
      121 ccaccctcga ggccgccgag ctgaccaagc tgctcgagaa cgtgttccgc tcggtgaaca
      181 tcgcgatggt gaacgagctg aaggtgctgt tcacgcgact gggaatcgac gtgtggcgcg
      241 tgatcgaggc ggccaagacc aagccgtttg gattccaggc cttttatccc gggccaggcc
      301 tcggcggcca ctgcctgccg gtcgacccgt tctacctcga ctggaaggcc cgcgagtacg
      361 acacgccgac cgagttcatc gagctcgccg gcgaggtcaa ccagaacatg ccctacttct
      421 gcgtggagaa gatcacgcgc gcgctgaacg atcactcgaa gccgctgcgc gggtcgaaga
      481 tcgccatctt cggcgtgtcg tacaaggccg gcgtcggcga cctgcgcgag tccccggcgc
      541 tgaagatcat ccgcctgctg ctcgcgcgcg gcgcggagat cgcgtactcg gacgagttcg
      601 tgccggcgct gcctgagttc ggcctcgagc acgcggacgt ggacgaggcg ctcgccggcg
      661 ccgatgcggc ggtgatcgtc accgcgcacc cggggctcga cgtcgcgagc gtggtggaga
      721 gctctccgct cgtcgtggac ttccgcggcg tcacgcgcgg catcgacgcc gccaacctcg
      781 tcaggctcta gcgcgtcagc gccggcgctc aggcgtcagg ccgcctcgaa cctcacgacg
      841 ccgtcctcct cgacctcgcg cacgcggccc tcgttctcga ggatgtccgt gtgaccgagg
      901 acctccgaca gcgtgaggta ggcctgcgtc acggccacgt tgccccagag cgcctgggcc
      961 agctcgtagg ccgtgcgcgg ccgctcctcg atcaggcggt ggagcttgcc cgcgcgcttg
     1021 gcgtgcagct tcaggcgcag ctcgatcagc ttgcggtgct cggcgatcgg ctcgccgtgg
     1081 ccgggcagca ccaggtcgag gtccatggcc gcggtccgct ccagcgagtc caggtaggcc
     1141 accagcgcct gggggcgctc gcgcacgtcg ggggccttgc cgtcccagag cggggtgacg
     1201 agcgggttcg aggagatgtg cgcgatcagg tggtcggcgg cgagcagcac gcggcgatcg
     1261 gggtcgtgga agaccgtgtc cgtcgggctg tggccggggc ggtggaggac ctcgagccgg
     1321 cggtcgcgca gctcgacgtg gtcgccgtcg tggagcacgc gggtcacctc ggcgcccgcg
     1381 ccccaggcgc ggaaggcgag cgacacggac tggagcgccc cgaccacgtc cggtgagatc
     1441 ccgtgtcgca gcatcagctc cgaggcgaag acgtcctggg cggccgtcgc ctcgctgtag
     1501 ttctccatga acggcacgag ccggtcgatc gccgcgacct cggcaccgga gcggcgcgcg
     1561 acgatgctca ccagcccgat gtggtcgatg tgctggtggg tcacgaggat caggccgatg
     1621 tcctcgagcc ggtggccgag cgcgcggagc tggctctcga gctcgtccag cgagcggccg
     1681 gagttcggtc ccgcgtcgac cagcgtcagc gggtcgtcct cgatcaggta gaggttcacc
     1741 cgcccgacgg cgaacggcgt cgggatcgga agggcgtgga tcccctcgtc aggcggcacg
     1801 gatcagcgcg agcacctcgt cgcgcttctg ctccatgtgc tccggcgaga cgagcgactc
     1861 gaggttgagg cgcaggagcg gctcggtgtt cgacgggcgc acgttgaagt gccagtcctc
     1921 gtagtcgatc gagacgccgt cgaggtgggt gatctccgcg tcggagtagc ggtcctcgag
     1981 ctcgcgcatc ctcgcctcgc cgtcggcgac ctcggagttg atctcgccgg agatgaagta
     2041 gcgcgagcgg aagtcggcca gcagctcgct catgcgcttc cccttctgcg agagcagctc
     2101 gaggatcagc agcgccggga tcgtgccgga gtcggcgcag tagaagtcgc ggaagtagta
     2161 gtggccggag acctcgccgc cgaacgcggc gccctcgtcg cgcatgcggg tcttgaagaa
     2221 cgcgtggccg acgcggttca cgtgggcgct gccgccggcg cgctcgacca cgtcggcgac
     2281 ggcgcggctg gcgcggacgt cgtagaggat cgcggcgccg ggctccttgg cgatgatcga
     2341 ctcggcgagc agcgcggtga ggaagtcgcc gtccacgaac tggccgttgt cgtcgatgaa
     2401 gaagcagcgg tcggcgtcgc cgtcccaggc aatgcccagc tcggcgccct cggagagcac
     2461 cttcttgacg atgaacgtgc ggttctccgg caggagcggg ttgggctcgt ggtcggggaa
     2521 ctcgccgtcg ggcgtccagt aggtggccgc ctggtcgagc gggaggcggt cgagcagcgg
     2581 gccgaccatc ggacccgcca ttccgttgcc gccgtcgagc acgaccttca tcgggcggat
     2641 cgcgtcgagg tccacgaact tcgccgcggc ggcgcggaac tcgtcggcca cgtcctcctc
     2701 cgcgtagtcg ccgcgcgcct cggccggcgg cccgggctcg ccctcctcga ctatccggcg
     2761 cagctccccg atgccggagt cgcccgagag ggcgatggcc ccgcgcttga cgagcttcgc
     2821 gccggtgtag cccttcgggt tgtgcgaggc ggtgcacatg agcccgccgt cgagctcacg
     2881 cgatccgacc gcgaaataga gcatctcggt gccgaccatg ccgacgtcga gcacgtcggc
     2941 accctcgtcg cgcatgccgt cggcgtagcg ggcggccagc tcgggggcgc tcaggcgcat
     3001 gtcgcggccg agcccgaggc gcatctcgcc gggcgggcgg ccctcgccat cggcgatcac
     3061 gcgcacgaac gcgcggccga cccggtacgc gacgtcggcg tcgatctgct cggggtgcag
     3121 gccgcggacg tcgtaggcct tgaagatcgc ggagtcgatc atgcggcggc gtaggcctcg
     3181 gtgagcgcgt cgtaggagtc ggagaggctc tgcgggagca cgcgcgtgcc ggagaccacc
     3241 ggcatgaagt tcgtgtcggc gccccagcgc gggaccacgt gcaggtggac gtggtgctcc
     3301 acgcccgccc cggccacctt gccctggttg atccccaggt tgaagccctc cgggccgtag
     3361 gcgcggcgca gagcgccgag ggagtcctgc gccatcgtca tcagctcgag gagggcaggg
     3421 ccctcgagct cctcgatcga cggcacgtgg cggtacggcg ccaccatcac gtgcccgttg
     3481 ttgtatggga acgcgttcag catcgtgaag cagtgctcgc cgcggcggac gatgtaggtg
     3541 ccgcggtcgt cgccggcctg cggcttggtg cagaagatgc actcgtcggc cttcgggccc
     3601 ttgatgtact cgaggcgcca gggcgcccac agtcgttcgt ccggcatgcg gtggatgact
     3661 ctattggccg ggcgcgcggg cgatgacggc catccggtcg cggaagtcgg gcgcccacat
     3721 gcggtcggcg acgccggtcg cggcggcggc cgcgacgagc gcgttggcca gcggcggcga
     3781 gtagccgccg atccgctcga ggtagtagcg gaccgtgaac gccttcggcg ccgtgccggt
     3841 ccagagcggc tcccagccgc ggcggcgcag cagcgtgagg aggctcgggc gggtgaagta
     3901 ctgcacgtgg gtcgggatca ccgaccacca gcgcgcgccc atgcggcggg cgaggcggct
     3961 gccggcgttc ggaagggcca ggtagaggat gccgccgggc tccagtagcc cggccatgcg
     4021 gtcgagggcg tcgtcggggc gggggaggtg ctcgatcacg tcgcccatcg cgatcgcgtg
     4081 gaagcggcgg ccgccgaggt cggcgtcgaa caggtcggcg cggaccacgt cgagcccgag
     4141 tcgctcgcgc gcgtaggagg aggcgaactc gcttggctcg acgccgagcg tgtcccagcc
     4201 acgctcgcgt gcctcggcta gcaggaagcc gacccagcag ccgaggtcga gcagggcggg
     4261 ggcggagccc ccggcctcgg cggccgcccc gggcgcgcgg gctccgccgg gcggtcgcgg
     4321 ggcgtagcgc tcgatccgct ccagcgccgt gcgggccgtg gcgcgctgcc cggcctcctc
     4381 gtccacgtag tcctccgact cggcctcgcc ataggcctcg ccgagctcgg cctcggacgg
     4441 gaagcggtcg agctgcatgt ggctgcacgc ggggcagcgg acgatgtcgc cgagcgccgc
     4501 gccgaagcgg tcggtcgtcg ggatcaggtc ctcggcgcgg gcgtccgcca cctcgaggtg
     4561 ggggatcagg acggtcgagg cgcaggctgc gcaggtggcc atcggggtca gatcgtatgg
     4621 tggccccatg cctgagcgcg atgtacgcgg tggtcgcgtc ggcctggtca gcgtcgtggc
     4681 gccgatgttc gacgaggagg agaccgtcga ggtcttctac gaccgggtgc ggcgagcgct
     4741 cgacggcgtg cgcttcgagc tcgtgctggt cgacgacgcg agccgcgacc gcacccctga
     4801 gctgctcgac cggctggcct cctcggaccc gcgcgtgaag gtcctccacc tctcgcgcaa
     4861 cttcggccac caggcggcga tcaccgcggg gctcgagcac gcgcggggcg aggccgtcgt
     4921 gatgctcgac ggcgacctcc aggacccgcc cgaggtgatc ccgcagatgc tcgaccgctg
     4981 gcgcgcgggc gccgacgtgg tctacggcgt gcgcgagagc cgggcgggcg agagccgctt
     5041 caagctcgtc accgccagct cgttctaccg ggtctttgcg cggctcacca agctcgacct
     5101 gcgcaagaac tccggcgact tccggctgct cgaccgccgc gcgctcgacg ccgtgctcgc
     5161 catgcgcgag cgcaaccgct tcctgcgcgg gatgacggtg tgggtcgggt tcgtgcagac
     5221 cgcggtgccg tacaagcgcg acgcgcgcta cgcgggggag accaagtaca cgctgcgcaa
     5281 gatggtgcgc ttctctctcg acgcagtggc gtcgttctcg tcgttcccgc tgcagatcgc
     5341 caccttcctg gggttcctgt gcgcgggctt cgcgttcctg ctgctgccgc tggtggtggt
     5401 cgcgcggctg ctcgagatct acgacgcggg cgtgccgtcg ctcctgttcg tggtgctgct
     5461 gctcggtggg atccagctca taacggtcgg gatcatcggc gagtacgtgg cgcggatcta
     5521 cgacgaggtc aagggacggc cgctgtacct cgtgcgcgac gcgcgcaacg tgaccgccga
     5581 gcagcccgac ggggcgccga gcgagccgca gcgcgtcccg gcgccgtagc gatgcgcgtc
     5641 gccgtgttgg gagccggcgt ggccgggctc accgccgcgt accggctgac ccagcgcggc
     5701 cacgtcgtcg acgtgtacga gcgctggcct ggcctggggg gccaggcggc gacgctcgac
     5761 gccggcgacg ggcacctgat cgagcgctac taccaccact ggttcacgag cgatcgccac
     5821 atcgtcgagc tctgcgagga gctcgggatc ggcgacgacg tggagtggcg gccgtcgagc
     5881 gtgggcatgt tcgccgacgg ggtcgcgcat ccgttcacca ccccggtgga cctgctgcgc
     5941 ttcaccccga tgtcgctgcc ggcgcggctg cggatggggt tggcggtcgt gtggatccag
     6001 cgccgccatc cgaccgtcga ggggttccac gacgtgacgg cgcgctcgtg ggtcgtgcgg
     6061 gcgatggggc gccaggcgtg ggacaaggtc tgggggccgc tgctgcacgg caagttcggc
     6121 gcgcgggccg acgagatcgc gatggcgtgg ctgtggggca agctcacgac gcgtcgccag
     6181 atcaagggcg aggaggcgcg caaggagctg ctggggtacc cgcggacgag cttcgagggg
     6241 ctgttcaggc gcctgcgcga cgagatcgag ggcggcggcg ggcgggtgct gatcgaccgg
     6301 ccggcggcct ccctggcccg ctctgacgag gggttcacgg tcgcgccggg ggcggtcgac
     6361 tcgttccggc gcgggcacga tccgcgctcg ttcgcggttg acggtgcgcc ggagcgctac
     6421 gacggcgtaa ttgcgaccgt tccgagcgac gtgttcgagc agctgctcga cccggacctg
     6481 gccacggcgg tgggcgagcc ctacctggag cgcgtgcggg ggaccgagta tcaggcggcg
     6541 ctgtgcctag tgctggagct cgaccggcgc ttcgggcgct tctactggct gaacgtggcc
     6601 gacgcgtcgc tgccgttcat cgggttgatc gagcacacga acttcgtcga gccggagcgc
     6661 tacggcgggc ggcggttcct gtacgtggcg aactacctcg agcagggcga tccgctgctc
     6721 gcgctctcgc ccgaggagct gctggagcac tacacgccgg ggctgcggcg cgtgaacccc
     6781 gagttctcgg ccggctgggt gcggaggagc tggctcttcc gcgagccggc ggcgcagccg
     6841 atcgtgcccg tcgggtacgc gcaccgcatc ccgcccctgc gcacgccggt gccgggcctg
     6901 gtcctggcga acaccacgca gatctatccc gaggaccgcg gcacgaacta cagcgtgcgg
     6961 ctcgggggcg aggcggtgga ggcgctgctc ggctagctgg gcccgagccg ctcgtcgatc
     7021 tccactctga gcgccgccgc gacgcgctcc agctggtcga tgaaaccggc tacgaggggc
     7081 gggcgctcgc gacttgcggg tagcgcaacc gcgaccgttc gcgagaccgg gggctcggcg
     7141 cgaaccttca ccagcgcgac atccgagcgc accgcgaccg cggccagggc tggaaccacc
     7201 gctatgccca ggccctccgc gaccagcccg agcttggccg tccaatcgcg cacgacgtag
     7261 gcgacgtcgg gcttccagtc cagccgcggc caggcaccga ggagcgcatc ggccgtgttc
     7321 gtgctcccgc tgatccatcg ctcgcccgcg aactcgacta cctcgacggt gcgccgcgac
     7381 gcgaacggat gacttcgcgc gaccgcaagc agcatcggat cgtcgagcag ccgcgtaagg
     7441 gtgaggtccg gcgcgtcgag ctcgccgccc aagggcgagc ccagcaccgc gaggtccagg
     7501 gcgccggcgc gcaggcggcg caactgcgtc ggagtcgtgc cctcacggag caggatctcg
     7561 accagcggct cctcgctcgc gtgccttgtc agcgcgcgcg ggaccagcgc cgctacagcc
     7621 gtcgggaacg cgccgacccg aagccgtccc cgcgtcgtcg cggcgagcga cgtcatctca
     7681 cgcgtggccg cgtcgacgtg gtcgagcgcc gcgttcgcgt gcgtcagcag ccgggcgccg
     7741 gccgcggtga gcctcacgcc gcggcggccg cgctcgaaca gcggtgcacc cgccgcgtcc
     7801 tccatcgccg ccacctgccg cgagatcgcc gactgcgcat agccgagggc ctcggctgcg
     7861 gcggtgaacg tgccgtgctc ggcaacggcg cgcagcaccg caaatccggt gagcgtgaat
     7921 gcaggcatct ggttacagca tggcgactat gcgaaatcgt cgttttgcag atggcacgcc
     7981 tggtgtgaga atcgcgcaca tgaacactcg agagaacaga gacacgatga gggcggtctt
     8041 cgagggtctg gcggcgggcg acggcacgcg gctcgtggaa gccatgtccg acgacttctc
     8101 ctggacgatc gccggggtgg ccacgcactg ggccgggacg tggcgcggca agcaggccgt
     8161 ccgcgaagaa ctcctggcgc ccctcttcgc gcagttcgcg gaccgctatg tgaacacccc
     8221 gctgtctttc acggcggagg aggatcgcgt ggtcgtcgag tcccgcggcc gggtcacgac
     8281 gaaggcgggg gcccggtacg acaaccacta ctgctacgtg tgccgcttcg acgacgacaa
     8341 ccgcctggct tcgatcgtcg agtacgccga cacggccctg atggaggcgg ctcttcaccc
     8401 tcccgaatcg cgagcgagga cgccgatcag gacctcgtag tccgggtcgt gcacgtcgcg
     8461 cgcggccggg tcgagctctt cgctcgcgat gccgtggagc tgcgcggtgg cggtgagcac
     8521 gttgccgaag gtctcgaccg acacctcccc gaacccctcc tcgaacagcc gctgcgcgga
     8581 ttgccgcgtg aagcgccagt agtcgcccca gctctcggcc tcctcgcggc acacacgcgt
     8641 gatccccggg atcgtgacga gcgcggtgcc gcccggcttc aggatccggc ggatggtcgc
     8701 gatcgcggcg gggacgtccc agatcaggag cagcgtctgg gtgcagacga cgcagtcgaa
     8761 gctcgcgtcc gggatctgcg gcgcgtcggt caggtcgccg acgatggtcg cctgcgggtt
     8821 gccctcctcg acgtggagca cgtcgacccg ctcgacgcgg tcgccgccga acgtgcgggt
     8881 gtagcgatcc tcgctgatct ccagcacgcg gccacggatg tcggcggcgt ggcgctcgag
     8941 gaagcggtcg atgtagtggc ggtcgaccgg ggtgccgcgc tcgaagccga agccggggct
     9001 gaccggggtc gtggtcccga ggtcgccgag gtcgagcgag ccgacgcgcg gcgcggtacg
     9061 cgcgcggcgc agcgcgcgcc cggcggactc gccgagcagc gcgcggcggg cgtcggcggt
     9121 ggggctgccg gactcggcgc ccgccgtgag cgcgccgagc agcgcccgcg cgacgtgcca
     9181 ggcgagccgg gcgggcggct cgtacggggc gtccacgcgg gggagcagga gcgggtccga
     9241 agcgggcgtg accgccgcgg gcgcgaccgt gaagcccgcg ccgtagcccg cggagcgcgc
     9301 ggccgcggcc acccgcgcgt cggcgacgcc gaagggatag gcgatcagct cgacgggtgc
     9361 gcccgcggct gcggcgagct cgtcgcggcc gtcggcgagc tcgtgggcca gctcgtcgtc
     9421 ggggagctcg gtgagcaccc gatggccgcg cgtgtggaag ccgatctcgt ggccggcggc
     9481 ggcgatgcgg gcgatgtcgg cgggggcgag ccttgccgag tcgccgccgg cggccgctcc
     9541 cgggaggccc gcggcttccc cgcccgcgcc gccgcccgcc gccgcgagcc gctccgccaa
     9601 gccgtcgcgt tccgccgccg gcagcgcctc catcgcggcg cgtgcttcgc tcgccggcat
     9661 cccccgccgc agcggctccg gcagtgcggc gccgcggtcc agcgccgcct ggagg
//
LOCUS       DASVPL010000171         5409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_136953, whole genome shotgun sequence.
ACCESSION   DASVPL010000171 DASVPL010000000
VERSION     DASVPL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136953"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VF517_08925"
     CDS             <1..484
                     /locus_tag="VF517_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053104.1"
                     /translation="SPGQRAEVREHLARARATARTLFPSFEAARAAGYVFAPRSYEKQ
                     KDHDYWHLSRPDYMEDSNYVDPTAPETLMYWKNPGGEPVLMAFVYRVPRSEPNPALGG
                     PIVQWHLHTSNGKLGRLKMSHVWLVSDLRVAFQHDLPLAAVEREHGVKLPRNGTGAGV
                     "
     gene            525..606
                     /locus_tag="VF517_08930"
     tRNA            525..606
                     /locus_tag="VF517_08930"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:559..561,aa:Tyr,seq:gta)
     gene            complement(629..1858)
                     /locus_tag="VF517_08935"
     CDS             complement(629..1858)
                     /locus_tag="VF517_08935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX8053105.1"
                     /translation="MGAPRATGEPGPRRGLAITLGVVLVLSLLLILAIVGPAGFVVGI
                     ALALLPLPIYVLLALWIDRYEPEPRRLLAWAFIWGAGAATLIALIVNSVGIELVGAEF
                     GSDVGEIYGGSVSAPVVEEGSKALALYGVYRWRRSELDGVLDGIVYAAMVGLGFATTE
                     NVLYYGTEAIEHDVGVGTIFVMRGVASPFAHPIFTAMTGIGLALAATATKRSRRFWAP
                     VLGLLGAMLLHSAWNTSSIDGGAFVLTFVFVMFPAFVALIVVALVARRRDGRRVAAYL
                     GPEVGRGLLSHEDVQALSTMSGRARARRAARASGGKPAAKARGELHRAATDLAFLRHR
                     LALGRQDPDAPAPAQQEAEYVARIAQLTGRLPAPPPQAAAPPPPPGPPPNWYPDPSGR
                     FRLRYWDGYSWTGHTSA"
     gene            1932..2501
                     /locus_tag="VF517_08940"
     CDS             1932..2501
                     /locus_tag="VF517_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8053106.1"
                     /translation="MRRLIVSEFVTLDGVMEAPGGEPSHPHSGWVFDFMSKEQEEYKL
                     QETLEAEALLLGRVTYEGFSAAWPERGGEFADKMNSMPKYVASTTLSEPLEWSNSTVL
                     GADVPEAVAKLKQGDGGPILVAGSRTLVHTLLEHGLVDELRLMVFPVVLGSGIRVFPE
                     SRDKLVMKLTDARTFDSGVQILTYEPAAA"
     gene            2539..3378
                     /locus_tag="VF517_08945"
     CDS             2539..3378
                     /locus_tag="VF517_08945"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HEX8053107.1"
                     /translation="MPPRRARVLLGALCAALTLLPIALTPTQAAPIRVAEFVARCDLS
                     HRAPDDPIVFPGKPGAAHSHDFYGNRRTSARSTVRSLQRGKTTCNPRADRAAYWFPTL
                     YENGRAVKPRRATFYYVTEIEDPSEVRSLPQGLRIIAGDARARRPLHPIVAEWACLGS
                     DVESDPTMVTCPDDADLELHLRFPDCWNGRDLDSPDHKSHMAYSRADRCPKSHPVPVA
                     QLKFKLTYPIGGGRGVRLSSGPGYTAHGDFLNTWDQRELNRRVSSCLRRVIKCGEDGR
                     PLE"
     gene            complement(3344..4261)
                     /locus_tag="VF517_08950"
     CDS             complement(3344..4261)
                     /locus_tag="VF517_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX8053108.1"
                     /translation="MSPQGRTVEEKIAGIASRRWGVVTWAELRRAGLSPDQIRWRVRI
                     GSLIRVHRGVYRVGHRAPCTEATYLAAVKACGEDAVLSGRAAAYLQGLLPKCNPPAPE
                     ATAPTERKIAGVRTRRRRLDRRDVTRLRGIPITTAPRTLVDLAAVLGLNDLARACHEA
                     GVRYRTTPRQVEEVLRRSPNARGAKKLRTVMHGEAKVTLSRLERRFLKLLSDAVLPLP
                     ETNRPAGGRRVDCRWPDHHLTVELDSYTYHSSRRAWEQDRRREREAYARRDQFRRYTW
                     GDVFEDTRPMLSELRELLATRAGGRPHRT"
     gene            complement(4327..>5409)
                     /locus_tag="VF517_08955"
     CDS             complement(4327..>5409)
                     /locus_tag="VF517_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053109.1"
                     /translation="VQRVAFQTPDGGTPSPCTINCHDGYRDPRWIPGTPNAGMITTSG
                     NGISVQNGKSLTPWAQTDGDFEGFDVSRAGNKIAIIATVDGQPAEGQHQQGVLQFFQG
                     SGPPPAAASLVCQAAIGDEKSQPRWSPDGTGITWSDHDGVWVSPAPTAGGNGACNLQP
                     RLLAAGATAPDWGQASVPAPPTGPTGPTGPTGPTGPTGPTGPTGPTGPTDPTGPTGPT
                     GPTDPQNPNDPQNPNDPNDQTGDKTAPQLSGVTLPKAKLVATLSKGYGVTFTTSEAGT
                     ATLTIAASGSNAKGLRIVMAKSVNVAVGSLKAANAGKYKVVAKFSKKAQAKLRNLRSV
                     KLSATLTFADAAGNTAATSRKITLKR"
BASE COUNT          741 a         1958 c         1933 g          777 t
ORIGIN      
        1 ctcgcccggg cagcgcgccg aggtgcgcga gcatctcgca cgcgcgcgcg cgacggcgcg
       61 gaccctcttc ccgagcttcg aggccgcgcg cgcggcgggc tacgtgttcg cgccgcgcag
      121 ctacgagaag cagaaggacc acgactactg gcacctctcg cggccggact acatggagga
      181 ctcgaactac gtcgacccca ccgcccccga gacgctcatg tactggaaga accccggggg
      241 cgagccggtg ctgatggcgt tcgtctaccg ggtgccgcgc agcgagccga acccggccct
      301 cggcggcccg atcgtgcagt ggcacctgca cacgtcgaac ggcaagctgg gccgtctgaa
      361 gatgagtcac gtgtggctcg tgagcgacct gcgcgtggcg ttccagcacg atctgccgct
      421 cgcggcggtg gagcgcgagc acggcgtgaa gctgccgcgc aacggcaccg gcgccggcgt
      481 ctgagcggcg cccgagcgcg tctacaatcc ccggccgatc gcccggcggg atacccaagc
      541 ggtcaacggg accagactgt aaatctggcg gctcagcctt cgcaggttcg aatcctgctc
      601 ccgccatctc cacgcccgcg gggcgggctc acgccgacgt gtggcccgtc cacgagtagc
      661 cgtcccagta gcgcagccga aagcggccgg acgggtccgg gtaccagttc ggcggtggac
      721 ccgggggcgg cggcggggcc gctgcctgtg ggggtggcgc cggcagccgg cccgtgagct
      781 gggcgatccg tgcaacgtac tcggcctcct gctgggccgg cgcgggcgcg tccggatcct
      841 gccggccgag cgccagccgg tggcgcagga acgcgaggtc ggtggcggcg cggtgaagct
      901 ccccgcgcgc cttcgccgcg ggcttgccgc ccgacgcacg cgccgcccgc cgcgctcgcg
      961 cgcggccgct catcgtcgac agggcctgca cgtcctcgtg actgaggaga ccgcgcccga
     1021 cctcgggccc gaggtacgcg gcgacccggc gcccgtcgcg gcggcgcgcg accagggcca
     1081 ccacgatcag cgcgacgaac gccgggaaca tcacgaacac gaacgtgagg acgaacgcgc
     1141 cgccgtcgat gctcgacgtg ttccaggccg aatggagcag catcgcgccg agcaggccga
     1201 gcaccggggc ccagaacctg cggctgcgct tggtcgccgt ggccgcgagc gcgaggccga
     1261 tcccggtcat cgcggtgaag atggggtgcg cgaacggaga cgccacgccg cgcatgacga
     1321 agatcgtgcc gaccccgacg tcgtgctcga tcgcctcggt cccgtagtag aggacgttct
     1381 cggtcgtggc gaagcccagg ccgaccatcg cggcgtagac gatcccgtcg agcacgccgt
     1441 cgagctcgct gcgccgccag cggtagacgc cgtagagggc cagcgccttc gacccctcct
     1501 ccacgaccgg cgcggagacc gagccgccgt agatctcacc gacgtccgac ccgaactccg
     1561 ctccaaccag ctcgatcccc acgctgttga cgatcagcgc gatgagggtg gccgccccgg
     1621 caccccagat gaacgcccac gcgaggagcc ggcgcggctc cggctcgtag cggtcgatcc
     1681 acagcgcgag cagcacgtag atcggcagtg gcagcagcgc gagggcgatc ccgaccacga
     1741 acccggccgg cccgacgatc gcgaggatga gcaggaggga cagcacgagg accacgccga
     1801 gcgtgatcgc gagcccgcgc cgcggccccg gttcgcccgt tgccctcggc gcgcccacct
     1861 cgggaagact gccaccgatg agcccgcggg cgagttgcgg tcctaccgat gacccctgag
     1921 gaggagaaga gatgagaagg ctgatcgtgt cggagttcgt gaccctggac ggcgtgatgg
     1981 aggccccggg cggggagccc tcgcaccctc acagcggctg ggtcttcgac ttcatgagca
     2041 aggagcagga ggagtacaag ctccaggaga cgctcgaggc cgaggcgctg ctgctcggcc
     2101 gcgtgaccta cgagggcttc tcggccgcat ggcccgagcg cggcggcgag ttcgcggaca
     2161 agatgaactc gatgccgaag tacgtggcgt cgacgacgct ctcggagccg ctcgagtgga
     2221 gcaactcgac ggtgctcggc gcggacgtgc cggaggccgt cgcgaagctc aagcagggcg
     2281 acggcgggcc gatcctcgtg gccggaagcc gcacgctcgt gcacacgctg ctcgagcacg
     2341 ggctcgtgga tgagctgcgg ctgatggtct tcccggtcgt cctcgggtcc ggcatccgcg
     2401 tgttcccgga gtcgcgggac aagctcgtca tgaagctgac cgacgcgcgc accttcgact
     2461 cgggggtgca gatcctcacg tacgagcccg ccgccgcgta gcccgcttcg caacggaggg
     2521 ccggtctacg atgagccggt gccccctcgc cgcgcccgcg tgctgctcgg cgcgctctgc
     2581 gccgcactga ccctcctccc tatcgcgctc acgcccaccc aggccgcgcc gatccgcgtc
     2641 gccgagttcg tcgcccgctg cgacctgagc caccgcgcgc cggacgaccc gatcgtgttc
     2701 cccggcaagc cgggcgccgc gcacagccac gacttctacg ggaaccgccg gacctccgcg
     2761 cgctcgacgg tcaggagcct ccagcgcggc aagaccacct gcaacccgcg cgccgatcgc
     2821 gccgcctact ggttcccgac gctgtacgag aacggccggg cggtgaagcc gcggcgggcc
     2881 acgttctact acgtcaccga gatcgaggac ccgtcggagg tgcgctcgct gccgcagggg
     2941 ctgcggatca tcgcgggcga cgcgagggcg cggcggccgc tccatccgat cgtggccgag
     3001 tgggcctgcc tcggctccga cgtcgagagc gatcccacga tggtcacctg ccccgacgac
     3061 gcggacctcg agctccacct ccgcttcccg gactgctgga acggtcgcga cctcgacagc
     3121 cccgaccaca agagccacat ggcctacagc cgcgccgacc gctgtcccaa gagccacccg
     3181 gtcccggtcg cccagctcaa gttcaagctc acctacccga tcggcggagg caggggcgtg
     3241 cggctctcgt ccggtcccgg ctacacggcg cacggcgact tcctgaacac gtgggaccag
     3301 cgcgagctca accgccgcgt atcgagctgc ctgcggcgcg tgatcaagtg cggtgaggac
     3361 ggccgcccgc tcgagtagcg agcagctccc gcagctcgct cagcatcggc cgcgtgtcct
     3421 cgaagacgtc accccaggtg tagcggcgga actgatcgcg gcgcgcgtat gcctcgcgct
     3481 cgcggcggcg gtcctgctcc caggcccggc gggacgagtg gtaggtgtag ctgtcgagct
     3541 ccacggtgag gtggtggtcc ggccagcggc agtcgacgcg tctgccgccg gcgggcctgt
     3601 tggtttccgg caacggcaga acggcatcac ttaggagttt taggaagcgg cgttccagcc
     3661 tgctcagggt gaccttggct tcgccatgca tgacggttcg gagcttcttc gccccgcgcg
     3721 cgttcgggct gcgccgcagc acctcctcga cctgccgggg agtggtccgg tagcgcacgc
     3781 cggcctcgtg gcacgcgcgc gccaggtcgt tcaggccgag tacggcggcg aggtcgacga
     3841 gcgtgcgtgg ggcggtcgtg atcgggatcc ccctgaggcg ggtcacgtcg cgccggtcga
     3901 gcctcctgcg ccgcgttctc acgcctgcga tcttccgctc ggtcggcgct gtcgcctcgg
     3961 gcgctggcgg attgcactta gggaggagac cctggaggta cgcggccgcg cggccgctga
     4021 gcaccgcgtc ctcgccgcac gccttgaccg ccgcgaggta ggtcgcctcg gtgcacggag
     4081 cgcggtgccc gacccggtac acgcccctgt gcacgcggat cagcgagccg atccgcaccc
     4141 gccacctgat ctgatccggc gagagccccg cccggcgcaa ctccgcccag gtcaccacac
     4201 cccaccggcg gctggcgatc ccggcgattt tctcctcgac tgtcctgccc tggggggaca
     4261 ctgtccccct aagtcaggaa ggaccgaagt tcgccccccg ggcaccgtgc ccggggggcg
     4321 ggtcggctag cgcttcagtg tgatcttgcg gctcgtcgcg gcggtgtttc cggcggcgtc
     4381 ggcgaaggtc agcgtggcac tgagcttcac gctccgcagg ttgcggagct tcgcctgtgc
     4441 cttcttcgag aacttcgcga cgaccttgta cttgccggcg ttcgccgcct tcagcgaccc
     4501 gaccgccacg ttgacgctct tggccatcac gatccggagg cccttcgcgt tcgagccgct
     4561 ggccgcgatc gtgagggtcg cggtgccggc ctcgctcgtc gtgaacgtca cgccgtagcc
     4621 cttgctgagc gtcgccacca gcttggcctt ggggagcgtc actccgctga gctgcggagc
     4681 cgtcttgtcg ccggtctggt cgttcgggtc gttgggattc tgcgggtcgt tcgggttctg
     4741 cgggtctgtc gggccggtcg gtcccgtcgg ccccgtcggg tctgtgggcc ccgtgggccc
     4801 ggtgggtcca gtcggccccg tcggtccggt cggacccgtc ggcccggtcg gcccggtcgg
     4861 ccccgtcggc ggcgcgggca cgctcgcctg gccccagtcg ggcgccgtcg cgcccgccgc
     4921 gagcaaccgc ggctggaggt tgcaggctcc gttgccgccg gcggtcgggg cgggggagac
     4981 ccacacgccg tcgtggtcgc tccaggtgat cccggtgccg tccggcgacc agcgcggctg
     5041 gctcttctcg tcgccgatcg cggcctggca gacgagcgat gcggccgccg gcgggggccc
     5101 cgagccctgg aagaactgga gcacgccctg ctggtgctgg ccctcggcgg gctggccgtc
     5161 gacggtcgcg atgatcgcga tcttgttgcc cgcgcgggag acgtcgaagc cctcgaagtc
     5221 gccgtcggtc tgggcccacg gggtcagcga cttgccgttc tggaccgaga tcccgttgcc
     5281 gctcgtggtg atcatcccgg cgttcggcgt gcccgggatc cagcgcggat cgcggtagcc
     5341 gtcgtggcag ttgatcgtgc acggggacgg cgtgccgccg tccggtgtct ggaacgcgac
     5401 gcgctggac
//
LOCUS       DASVPL010000172         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_295229, whole genome shotgun sequence.
ACCESSION   DASVPL010000172 DASVPL010000000
VERSION     DASVPL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295229"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="VF517_08960"
     CDS             complement(<1..1572)
                     /locus_tag="VF517_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX8053110.1"
                     /translation="MNGSLEQLSDRARGMLAGAPPPEAPEPRPIEREGAPRPHFSAFD
                     DAQLGRAVELANAWWRLADERGGEEGVRAVLDEAERMIAEGEVPGLVKYALQLFMTHH
                     PRGRLLGMPTLAEIQPETASARATAQALADADPGDPEKQLWYFREDELANEHHWHWHR
                     VYPARRNDRGEFIPAERQGELFVYMHQQMLARYDTERIAAGLDPVEPLDYTEPVPEGY
                     ESSMPVPFPPREPNQSVDPGDVAWLDARRAEVEAAIAQLDPHGEAERITAINTLGAVI
                     EAAHLVSDPDEASGLHNSGHGALAALSPQPRGVMRATTQAIRDPVFWRWHRRIDDFAF
                     RWQERFPGAHDLTDGAAPVEIRDADVILALHRDIAGADTHEFDYQGWGQGRFGGTAFD
                     AEPGEPATPELVTRMVTDAREVDLIDPQTGKIVRDPETQLPVKTSIEEEHLEHDELVT
                     FIRVRNTVGRPVDATLRVFLVADARAEERRFWIEMDKFTVALEAQERRVVARPGWLSS
                     VIRKPARMSGQSIPTP"
     gene            complement(1628..>2316)
                     /locus_tag="VF517_08965"
     CDS             complement(1628..>2316)
                     /locus_tag="VF517_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8053111.1"
                     /translation="IHSPIRRELIGDGEARPLDAVAWRCMSTPPPGVDVLDEARQVWG
                     RGGEFGWVPLSDGRVYWFAAERLRPEAEHPGGRIGELVERFGGWTPEIEPLIAAADEE
                     RILRTELLARTAPRSWSRGPVALLGDSAHAMAPNLGQGACQALEDAVVLGRVLTGAED
                     PVRALREYSRARVPRAKAVSAISEWTGSMLYVNNGLLWRLRDLAFAAAPATALRMQLD
                     TVMRSRALLR"
BASE COUNT          282 a          880 c          841 g          313 t
ORIGIN      
        1 cggggtgggg atcgactgcc cgctcatccg cgcgggcttg cggatcaccg acgagagcca
       61 cccgggccgc gccacgaccc ggcgctcctg cgcctcgagc gccaccgtga acttgtccat
      121 ctcgatccag aaacgccgct cctcggcgcg agcgtccgcg accaggaaca cgcgcagggt
      181 cgcgtcgacg ggccggccca cggtgttgcg gacccggatg aaggtgacca gctcgtcgtg
      241 ctcgaggtgc tcctcctcga tgctcgtctt caccggcaac tgggtctccg ggtcgcgcac
      301 gatcttcccg gtctgcgggt cgatcaggtc cacctcgcgc gcgtcggtga ccatccgcgt
      361 gacgagctcc ggcgtcgccg gctcgcccgg ctcggcgtcg aaggccgtgc cgccgaagcg
      421 cccctgcccc cacccctggt agtcgaactc gtgcgtgtcg gccccggcga tgtcgcggtg
      481 cagggcgagg atcacgtccg cgtcgcggat ctcgaccggc gccgccccgt cggtcaggtc
      541 atgggcgccc gggaagcgct cctgccaccg gaacgcgaag tcgtcgatgc gccggtgcca
      601 gcgccagaac accgggtcgc ggatcgcctg ggtggtggcc cgcatcacgc cgcgcggctg
      661 cgggctcaac gccgccaggg cgccgtgccc gctgttgtgg agcccggacg cctcgtccgg
      721 gtccgacacg aggtgtgcgg cctcgatcac ggcgcccagc gtgttgatgg ccgtgatgcg
      781 ctcggcctcc ccgtgggggt cgagctgggc gatggcggcc tcgacctcgg cccggcgcgc
      841 gtcgagccac gccacgtcgc cgggatccac gctctggttg ggctccctgg gcgggaacgg
      901 cacgggcatg ctcgactcgt agccctccgg gaccggctcc gtgtagtcga gcggctcgac
      961 cgggtcgagg ccggcggcga tccgctcggt gtcgtagcgg gcgagcatct gctggtgcat
     1021 gtacacgaac agctccccct ggcgctcggc gggaatgaac tcgccgcggt cgttgcgcct
     1081 ggcgggatag acgcggtgcc agtgccagtg gtgctcgttg gccagctcgt cctcgcggaa
     1141 gtaccagagc tgcttctcag ggtcgccggg gtcggcgtcg gcgagcgcct gggcggtcgc
     1201 gcgcgccgag gcggtctcgg gctggatctc ggccagcgtc ggcatcccga gcaggcgccc
     1261 gcgcgggtgg tgcgtcatga agagctggag cgcgtacttg acgagaccgg gcacctcgcc
     1321 ctcggcgatc atgcgctcgg cctcgtcgag caccgcgcgc accccctcct cgccgccgcg
     1381 ctcgtcggcg agccgccacc acgcgttcgc cagctccacc gcgcggccga gctgagcgtc
     1441 gtcgaaggcg ctgaagtgcg gccggggagc cccctcgcgc tcgatcggcc ggggctcggg
     1501 ggcctccggt gggggcgctc cggcgagcat cccgcgcgcg cggtcgctga gctgctctag
     1561 agacccgttc accccgatgc tcctcgcccg gcgacactag tccccgcggc cggagcgcgc
     1621 aagccgatta ccgcaaaagc gcgcggctgc gcatcaccgt gtcgagctgc atccgcagcg
     1681 ccgtcgcggg cgcggccgcg aaggcgaggt cgcgcagccg ccacagcagc ccgttgttca
     1741 cgtagagcat cgagccggtc cactccgaga tcgcggacac cgccttcgcg cgcggcacac
     1801 gcgcgcgcga gtactcgcgc agggcccgga ccgggtcctc ggcgccggtg agcacgcggc
     1861 cgagcaccac cgcgtcctcg agcgcctggc aagcgccctg accgaggttc ggcgccatgg
     1921 cgtgcgcgga gtcgccgagc agggccaccg gtccgcggct ccagctccgc ggcgcggtgc
     1981 gcgcgagcag ctcggtgcgc aggatccgct cctcgtcggc ggcggcgatc agcggctcga
     2041 tctccggcgt ccagccgccg aagcgctcga ccagctcgcc gatccgcccg ccggggtgct
     2101 cggcctcggg gcgcagccgc tcggccgcga accagtagac ccgcccgtcg gagagcggca
     2161 cccagccgaa ctcgccgccc cgcccccaga cctgccgcgc ctcgtcgagc acgtccacgc
     2221 cgggcggcgg ggtcgacatg caacgccacg cgaccgcgtc gagcggccgc gcctcgccgt
     2281 cgccgatcag ctcgcggcgg atcggcgagt ggatgc
//
LOCUS       DASVPL010000173        12469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_60494, whole genome shotgun sequence.
ACCESSION   DASVPL010000173 DASVPL010000000
VERSION     DASVPL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60494"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VF517_08970"
     CDS             complement(<1..480)
                     /locus_tag="VF517_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX8053112.1"
                     /translation="MSTTEHERFEQDGAAYLLGALPPLEAEVFERHLMACATCQRELE
                     RLRPAAQALPRAVEAFEPPPSLKRSLMDVVEAEAAAAGTGAPLAEPARTEPARTEPAP
                     PPPLERRSRTSFYPKGVRAPGWLAGLRPRAALALASVVLLAGVALGLGLAQGGGDDAE
                     "
     gene            complement(477..1040)
                     /locus_tag="VF517_08975"
     CDS             complement(477..1040)
                     /locus_tag="VF517_08975"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8053113.1"
                     /translation="MDVHRLGDEELMDLVAEGDPRAFEAIYDRHGRAAFSLAYRLAGD
                     RTAAEDIVQEALLSIWRSRVRYQRERGSVRTWILGIVHNRGIDNLRRSNVHQRRRASA
                     EGIEEWKEAPERTDDEAIQRDEAGTVRDALRTLPEPQERVIQLAYFGGFTHTQIAEML
                     DLPVGTVKGRMRLGLEKLRLTLSGEAA"
     gene            complement(1073..1399)
                     /locus_tag="VF517_08980"
     CDS             complement(1073..1399)
                     /locus_tag="VF517_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053114.1"
                     /translation="MSEVERLLHYALAPVEPPGTLTARLEQRLTDLTGAAVDELAEFD
                     QAALRDPRHWARLVTAGVVAGSAGGALILVRARQKHRRREAHGLRAIQKGMREVASDV
                     RNRLTS"
     gene            complement(1392..1931)
                     /locus_tag="VF517_08985"
     CDS             complement(1392..1931)
                     /locus_tag="VF517_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8053115.1"
                     /translation="MPDEPDKAELDRAFSDLYRAHLRDVYSYAYYRIGNHHDAEDLTE
                     HTFLQAYRHFARAQAESDGRPLRPWLIRIAHNLAANYHRDRSRRPQTHLEDASIVSAP
                     HQTDQLVEGREELQHVLEGVAGLADDRREALIMRFALGMDNREIARALGRSEGATKVL
                     IHRAIRQLEERLKEEHGYE"
     gene            complement(1961..2710)
                     /locus_tag="VF517_08990"
     CDS             complement(1961..2710)
                     /locus_tag="VF517_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8053116.1"
                     /translation="MALERHHERIRTRGINPVVYWVTRAVLQPFLLIWFRTARIGREH
                     IPSSGGVVLAANHKSFLDPFLIGLCLRRPIYFVAKRELFDNRLVGWFLNCLGAFPVRR
                     GESDEEMVATARAVLERGDALVIFPEGTRVRDPERLAEPKRGVGRFALECGVPVVPIA
                     VHGTERARSGWIVRPVKVRVRCGRQLTFPRVESASPRLAAAVTERIWPCVELQYRWLE
                     QLPALETRPPLVPRPTLALLGTGRNDDRRAA"
     gene            2756..3289
                     /locus_tag="VF517_08995"
     CDS             2756..3289
                     /locus_tag="VF517_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053117.1"
                     /translation="MTLQRSLRGGLAGGVAAAVWAGQLPLDKRVFGSDFDDVELMGKL
                     VTRGPQWPLAGVALHVQNGIVFGAVYAQLKPFLPGPPVLRGVVAGLAEHVALWPLTRV
                     TDRYHPARRDLPQIGGDWRAFAQATWRHALFGALLGALEHALNDRSADEPPEIPVSSN
                     GHGDIEQVAGATATSPA"
     gene            3303..3375
                     /locus_tag="VF517_09000"
     tRNA            3303..3375
                     /locus_tag="VF517_09000"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3336..3338,aa:Thr,seq:tgt)
     gene            complement(3377..4225)
                     /locus_tag="VF517_09005"
     CDS             complement(3377..4225)
                     /locus_tag="VF517_09005"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8053118.1"
                     /translation="MDRPATLTAALVVALVATALAAAVAAAGIGRAEQGRDAAAPQTR
                     RTSEPRRLEPVERLVGEPRVVSRAPAPREEAKRPATPPGFGVVRVRSGRSVRLYAKPK
                     GHAIGSAGARTQFGSPAVMSVVRRQGRWLAVTTTALPNGKLAWVRADSPAVDRGRVRT
                     AISVDLSERTVELRRGKKLVKRLRVAVGRPGSATPRGRFAITDKIPGSRYGSYYGCCI
                     LALNGTQPNTPPGWHGGNRLAIHGTNAPGSIGQASSAGCLRAADADLRTLMRSVPLGT
                     PVFVHG"
     gene            complement(4301..4963)
                     /gene="sigH"
                     /locus_tag="VF517_09010"
     CDS             complement(4301..4963)
                     /gene="sigH"
                     /locus_tag="VF517_09010"
                     /inference="COORDINATES: protein motif:HMM:NF006148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HEX8053119.1"
                     /translation="MAVSAPSIRSNHQSPLEVDDHYLVALAKSGKRDAYDRIVRRYYG
                     FVRLKASSYFLIGGDSDDLIQEGLVGLYKAVRDYRSDRESSFRNFAELCITRQIITAV
                     KTSTRNKHTPLNQYVSFSQTPASAGGDGEPTLDEVLPGPTAHDPVNQVISSEELHSLV
                     ACLSSVLSELESSVLSLYLDGHSYEAIGERLACDTKTVDNALQRVKRKVGAHLATRNV
                     ML"
     gene            complement(5099..5809)
                     /gene="rlmB"
                     /locus_tag="VF517_09015"
     CDS             complement(5099..5809)
                     /gene="rlmB"
                     /locus_tag="VF517_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX8053120.1"
                     /translation="MIVYGRNPVREALRGPRRVHPVWVSKNAAREPWLRGIDVRVADA
                     AELDSRAGSPDHQGICAEVSEFEYADPGGLLADENAVVVALDQVQDPRNLGAICRVAE
                     CAGAAGLVIGERRTAQVTPVVSHTSAGAIEHLPIARVRNLADYLAEAKRAGAWIYGAA
                     ASPEAVPHDQPDYSGRVVLVLGSEGRGLRPRVAAACDQLVALPLRGRIESLNVSAAAA
                     ALLYGILHSGRPGLDNVR"
     gene            complement(5806..7158)
                     /gene="cysS"
                     /locus_tag="VF517_09020"
     CDS             complement(5806..7158)
                     /gene="cysS"
                     /locus_tag="VF517_09020"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX8053121.1"
                     /translation="MIAIRDTLSGELRELAPREEGRVDIYACGPTVYAPIHVGNARPY
                     VVFSLLKRFLEYRGYTATLVINVTDVNDKIYDAAREAGVPSEQLAREMTERYERDTAR
                     LGLGRPDHEPRASETVPAIVELIGALVDRGHAYESRGDVYFRVASFAEYGKLSNRSLE
                     EADQGEPDDVGGDKESPHDFALWKAEKEGEDTAWDAPWGRGRPGWHIECSAMAEELLG
                     LDFEIHGGGSDLVFPHHENEIAQTEAGRGEPLARLWMHNGMVRFADEKMSKSVGNIRG
                     LGEALDQVGTDTLVMYFAGGHYRQPIEFSPSALDQAAASVRRVRELVRRLDPAVGTEG
                     IEPYEGRFLDALANDFNTPAALAELFGWVAEANRRLDAGERIGVGALRDMLWLLGLEQ
                     LLEAEDEAPDPDAERLLAEREAARAERDFATADARRDELRERGWEVRDTPEGPRLVRA
                     "
     gene            complement(7155..7634)
                     /gene="ispF"
                     /locus_tag="VF517_09025"
     CDS             complement(7155..7634)
                     /gene="ispF"
                     /locus_tag="VF517_09025"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX8053122.1"
                     /translation="MSARTGIGYDSHRLVEGRPLILGGVRVESELGLAGHSDADALTH
                     AIIDAVLGAASLGDIGLHFPDSDERYRGADSLALLDEVRALVSDQGFAVANVDSTVVC
                     DAPRLGPYRDRIRETLAAPLGIDAARVGVKFTTNEGMGWIGRGEGIAAMAVATLERP"
     gene            complement(7631..8458)
                     /locus_tag="VF517_09030"
     CDS             complement(7631..8458)
                     /locus_tag="VF517_09030"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX8053123.1"
                     /translation="MLTDLHVHLRPDEPEATAAEYFTTANAERYLDVAAERGVGALGV
                     SEHVHRFEQALSVWRHPFWQASATDDLDAYCEFVRDRTSLALGIEADYVPGAEDRMAN
                     LLEERDWDYVVGSVHFLRDSAVDMRGEWDIWTHTDPEKVWRRYFETLGEAARSGMFDI
                     LAHPDLVKVWGRERPQPDGDLRRFYDLAMDGIADSDVAIEVSTAGLRKPAGEIYPAPA
                     FLEMCLEAGRPVALSSDAHVPGDLAYRYDQALEFLESAGVGEIAVFERRERRLEPLG"
     gene            complement(8452..9099)
                     /gene="ispD"
                     /locus_tag="VF517_09035"
     CDS             complement(8452..9099)
                     /gene="ispD"
                     /locus_tag="VF517_09035"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX8053124.1"
                     /translation="MTVVGIVPAAGRGERLGEGVPKALVVCAGRPLLEWSLEVLERVC
                     DRVVVAVPPGYGDAAPDRVTGGDSRSESVRNALAAAPEATIAVVHDAARPFVTPELVH
                     DCVAAVRHEGFDGAVAAARVTDTVKEAGDDMRVERTLDRSRLWAIQTPQVFDAGVLRR
                     ALDAPAETLAAATDDASLVEAAGGSVRIVEAPPENVKVTTPLDLERAERLLAARC"
     gene            complement(9113..9724)
                     /locus_tag="VF517_09040"
     CDS             complement(9113..9724)
                     /locus_tag="VF517_09040"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX8053125.1"
                     /translation="MTPKRGEGPISETDWDQSVEDLEIEREAPHIDYKIGDQVVYPHH
                     GAGKVLKKEQKEVLGEKREYLTIKILHNDMTVMVPCENAGKAGLRRVIGDDDVRKVVA
                     VLCDDVSQMPKNWNRRFKHNRDKIKTGDVFELAEVVRNLAIREADKGLSTGEKQMYTR
                     AKKILASELMYALDMEEGEAEEHLESLISDAHAERNGAPAAAG"
     gene            complement(9721..10830)
                     /gene="disA"
                     /locus_tag="VF517_09045"
     CDS             complement(9721..10830)
                     /gene="disA"
                     /locus_tag="VF517_09045"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEX8053126.1"
                     /translation="MQHAGDEVVDLEHRQEPRLVKALEMVAPGSALREGIDHILHART
                     GALICIGDNEDLSFLYSGGIKVEVDYTPALLYQIAKMDGAIILSANATKICWANVQLM
                     PDPTIASLETGTRHRTAERVSKQTGALVIAVSQRRDVVSLYIDGGKYVLEEIPVVLAK
                     ANQALATLEKYRTRLEQVSTRLTALEFEGGATLHDVLTVLQRAELVTRMAVEIERYIV
                     ELGTEGRLIEMQLEETMIGAASDKASLVHDYIAQESEDQFALVVDQLARLPHEDLLDF
                     GRLAELLGYDRKLNTLDHPVSPRGYRILGRIPRLPKLVIGAIVGVFEGLDEILAATDA
                     ELEAVEGVGDLRAKDIREGLRRLQEINLVDRYLQT"
     gene            complement(10913..>12469)
                     /locus_tag="VF517_09050"
     CDS             complement(10913..>12469)
                     /locus_tag="VF517_09050"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX8053127.1"
                     /translation="AARHDGTLRVATIAPFLPEPADEFRIVRHASVTGAFAVAEGLQV
                     DEAHGYSPPDYEPSGTWLRSSTLPLVSVADAAVDEGTGTVEVEVRLSRATWRRVAVVA
                     ATRDDSAEAPGDYTRRSQVVTFEPGELVRRVAIPIVDDAIDEPDETFSVYLGEQGGAA
                     PGDMSATVTIADDDEPAPDPQPDPDPEPNPDLDPDPDSDADPGPGAGPDPDPDPDPDL
                     DPDPNPDSDPGPDTGPDPNPHPDPDLDPDPNPDSGPGPDTGPDPGPDPDPDPKPDPDA
                     DPGAGPGPAPDPHPDPDPDPQPGPGPGPDPEPGAEPTPQPKPDLDDGRGSTPNSGHEP
                     SAGPRTAPDAPSVGTPDTPGGDEAELPAWPGLDPATPARDPATPAGRFGRNPCPDELA
                     PRSSFRPGRHPIHASRTALRFRGIAWERGCGALARVTVAIARREGGGTGLCRYLQPSG
                     RLGRVVSCRRPTYVVARGTTSWTFTRTGYHRPGTWTARIRAVDRAGNAEKKVLKPNPL
                     SRNFVTFKIR"
BASE COUNT         1598 a         4389 c         4634 g         1848 t
ORIGIN      
        1 ctcggcgtcg tccccgccgc cctgcgccag gcccaggccg agcgcgacgc cggcgagcag
       61 cacgaccgag gcgagcgcga gcgcggcccg cgggcgcagt ccggcgagcc agcccggcgc
      121 gcgcaccccc ttcgggtaga acgaagttct agaacgtcgt tctagtggtg gagggggtgc
      181 gggctcggtg cgggcgggct cggtgcgggc gggctcggcc agcggcgcgc cggtccccgc
      241 cgcggccgcc tcggcctcga ccacgtccat cagcgagcgc ttgagcgagg gcggcggctc
      301 gaaggcttcg accgcgcgcg gcagcgcctg ggccgccggg cgcagccgct cgagctcgcg
      361 ctggcacgtc gcgcaggcca tcaggtgacg ctcgaagacc tccgcctcga gcggcggcag
      421 cgcgcccagc aggtaggccg cgccgtcctg ctcgaagcgc tcgtgttcgg tggtgctcat
      481 gccgcctccc cgctcagtgt caggcggagc ttctcgagcc cgagccgcat ccggcccttg
      541 acggtgccga ccggcaggtc gagcatctcc gcgatctggg tgtgcgtgaa gccgccgaag
      601 tacgcgagct ggatgacccg ttcctgcggc tccgggagcg tacgcagggc gtcgcggacg
      661 gtgccggcct cgtcgcgctg gatcgcctcg tcgtccgtgc gctccggcgc ctccttccac
      721 tcctcgatcc cctccgcgct ggctcgcctg cgctggtgca cgttgctgcg gcgcaggttg
      781 tcgatcccgc ggttgtggac gatgccgagg atccaggtgc gcacgctgcc ccgctcgcgc
      841 tggtagcgga cgcggctgcg ccagatcgag aggagcgcct cctggacgat gtcctcggcg
      901 gcggtgcggt cgccggcgag gcggtaagcc agcgagaacg ccgcgcggcc gtgacggtcg
      961 tagatggcct cgaacgcgcg cgggtcgccc tcggccacaa ggtccatgag ctcctcgtcg
     1021 ccgagtcgat gtacgtccac ggccgcttct acgcgcgggc ctcgacgacg gatcagctcg
     1081 tcaggcgatt ccgcacgtcc gaggcgacct cgcgcatccc cttctggatc gcgcgcaggc
     1141 cgtgagcctc gcggcggcgg tgcttctggc gcgcgcgcac gaggatcagc gcgccgccgg
     1201 cgctcccggc cacgacgccc gccgtgacga gccgtgccca gtggcgcggg tcgcgcagcg
     1261 cggcctggtc gaactcggcg agctcgtcca cggccgcgcc ggtgaggtcg gtcagacgct
     1321 gctcgaggcg ggcggtgagc gtgccgggcg gctcgacggg agcgagcgcg tagtgcagca
     1381 gccgttcgac ctcactcata cccgtgctcc tccttcaatc gttcctcgag ctggcgtatg
     1441 gctcggtgga tcaacacctt cgtggcgccc tcggagcgcc cgagcgcgcg cgcgatctcg
     1501 cggttgtcca tgcccagggc gaagcgcatg atgagggctt cgcgacgatc gtccgccagt
     1561 cctgcaacgc cctccagcac gtgctggagc tcctcgcgcc cctccacgag ctggtcggtc
     1621 tggtgcgggg cgctgacgat cgacgcgtcc tcgaggtggg tctgcggccg gcgcgagcgg
     1681 tcgcggtggt agttggcggc gaggttgtgg gcgatgcgga tcagccacgg gcgcagcggg
     1741 cgcccgtccg actccgcctg cgcgcgcgcg aagtggcggt acgcctggag gaaggtgtgc
     1801 tcggtgaggt cctcggcgtc gtggtgattg ccgatccggt agtacgcgta gctgtagacg
     1861 tcgcgcaggt gcgcccggta gagatcgctg aacgcgcggt cgagctcggc cttgtccggc
     1921 tcgtcgggca ctcacgcgag actagacctc cgcggcgctc ctacgcggcg cggcggtcgt
     1981 cgttgcggcc ggtgccgagc agcgccagag tcgggcgcgg cacgagggga ggcctggtct
     2041 ccagggccgg gagctgctcg agccagcggt actggagctc gacgcagggc cagatccgct
     2101 cggtcaccgc ggcggcgagc cgcggcgacg cgctctccac ccgcgggaag gtgagctggc
     2161 ggccgcagcg cacgcggacc ttcaccggtc ggacgatcca tcccgagcgc gcgcgctcgg
     2221 tgccgtgcac cgcgatcggg accaccggga cgccgcactc gagggcgaag cgcccgacgc
     2281 cccgctttgg ctccgcgagc cgttcggggt cgcggacgcg cgtgccctcg gggaagatca
     2341 ccagcgcgtc accgcgctcg aggaccgccc tggcggtcgc gaccatctcc tcgtccgact
     2401 cgccccgcct gaccggaaac gcgcccaggc agttgaggaa ccagccgacc agccggttgt
     2461 cgaacagctc gcgcttggcc acgaagtaga tcggccggcg caggcagagc ccgatcagga
     2521 acgggtcgag gaagctcttg tggttcgcgg ccagcacgac cccgccggag ctcgggatgt
     2581 gctcgcggcc gatccgggcc gtgcggaacc agatcagcag gaacggctgg agcaccgcgc
     2641 gggtgaccca gtacacgacg gggttgatcc cgcgggtgcg gatgcgctcg tggtggcgct
     2701 cgagggccat gggctgagag ggacaccggg ggtccgcgct cgttacgctc actgcatgac
     2761 gttgcagagg agcttgcgtg gaggtctggc cggcggagtg gccgcagccg tgtgggcggg
     2821 ccagctcccg ctcgacaagc gggtcttcgg ttcggacttc gacgacgtgg agctgatggg
     2881 caagctcgtg acccgcgggc cgcagtggcc gctggccggc gtggcgctgc acgtccagaa
     2941 cgggatcgtc ttcggggcgg tctacgcgca gctcaagccg ttcctccccg gcccgccggt
     3001 gctccgcggg gtcgttgcgg ggctggccga gcacgtcgcc ctgtggccgc tcacgcgcgt
     3061 gaccgaccgc taccacccgg cgcggcgcga ccttccgcag atcggcggcg actggcgcgc
     3121 cttcgcccag gccacctggc gccacgcgct cttcggcgcc ctgctcgggg ccctcgagca
     3181 cgcgctgaac gaccgcagcg ccgacgagcc gcccgagatc cccgtgtcct cgaacggcca
     3241 cggggacatc gagcaggtcg cgggcgcgac ggccacctca ccggcttaga ctcggccgcg
     3301 ccgccggagt agctcagctg gtagagcagc cgccttgtaa gcggcaggtc aggggttcga
     3361 gtcccctctc cggctcctaa ccgtgcacga agaccggcgt gccgagcggg acgctgcgca
     3421 tgagcgtgcg caggtcggcg tcggcggcgc gcaggcagcc ggccgaggaa gcctggccga
     3481 tcgagcccgg cgcgttggtg ccgtggatcg cgaggcggtt gccgccatgc cagccgggcg
     3541 gcgtgttcgg ctgggtgccg ttgagggcga ggatgcagca gccgtagtag gagccgtagc
     3601 gcgagccggg gatcttgtcg gtgatcgcga agcggcccct cggggtcgcc gacccgggcc
     3661 ggccgacggc gacgcgcagt cgcttgacga gcttcttgcc gcgcctcagc tcgacggtgc
     3721 gctccgagag gtcgaccgag atcgcggtgc gcacgcggcc gcgatccacg gcgggcgagt
     3781 ccgcgcggac ccaggcgagc ttgccgttgg gcagggcggt ggtggtcacg gcgagccagc
     3841 ggccctggcg gcgcacgacc gacatgacgg ccggcgagcc gaactgggtg cgcgcgccgg
     3901 ctgagccgat cgcgtggccc ttcggcttgg cgtagagcct cacggagcgc ccgctgcgga
     3961 cgcggacgac accgaagccg ggcggcgtgg cggggcgctt ggcctcctcg cgcggggcgg
     4021 gggcgcggct gaccacgcgc ggctcgccca ccagtcgctc gaccggctcg agccgccggg
     4081 gctcgctcgt ccggcgggtc tgcggggcgg ccgcgtcgcg gccctgctcg gcgcgcccga
     4141 tgcccgccgc cgcaacggca gcggccagcg cagtcgcgac gagggcgacg accagcgcgg
     4201 cggtgagtgt ggcgggcctg tccatggcct gcctgggatg tcggcgcgca tgggcgccgg
     4261 ctcaagtccg cgtacgaggg cggcgcccag ccgcccccgc ctagagcatc acgttgcgcg
     4321 tggcgagatg cgcgccgacc ttgcgcttga cgcgctggag ggcgttgtcc accgtcttcg
     4381 tgtcgcaggc cagtcgctcg ccgatcgcct cgtaggagtg accgtcgagg tacagcgaca
     4441 gcaccgagct ctcgagctcc gacagcacgc tcgacaggca ggcgacgagg ctgtgcagct
     4501 cctcggagga gatcacctgg ttgaccgggt cgtgcgcggt cgggccgggc aggacctcgt
     4561 cgagggtcgg ctcgccgtcg ccgccggcgc tcgccggcgt ctgcgagaac gacacgtact
     4621 ggttgagcgg cgtgtgcttg ttgcgggtgg aggtcttaac cgcggtgatg atctggcgcg
     4681 tgatgcacag ctccgcgaag ttgcggaagc tcgactcgcg atcgctgcgg tagtcgcgga
     4741 ccgccttgta gagcccgacc aggccctcct ggatcaggtc gtcggagtcg cccccgatca
     4801 ggaagtacga cgaggccttg agacgcacga agccgtagta gcgacgcacg atgcggtcgt
     4861 atgcgtcgcg cttcccggac ttcgccagcg cgacgaggta atgatcgtca acctcgagtg
     4921 gagattgatg gttactgcgt atcgacgggg cggatacggc cacagcatgc tcccctccca
     4981 gcccctacgg gactgaaaga ctgggcgcga cgtacctccc ctggtcgtcg ccagattttc
     5041 gcccgcctga gtctgtacct caggtcaagc cttatctgcg gcggcgagta tccccgtgtt
     5101 atcggacgtt gtcaagtccg ggtcggccgg aatgcaagat tccgtacagc agggccgcgg
     5161 cggcggcgga cacgttgagc gattcgatgc gcccccgcag cgggagcgcc acgagctggt
     5221 cgcaggcggc ggccacgcgc gggcgcagcc ccctcccctc cgagccgagc accaggacca
     5281 cgcgcccgga gtagtccggc tggtcgtggg gcaccgcctc gggcgacgcg gcggcgccgt
     5341 agatccaggc gccggcccgc ttcgcctcgg ccaggtagtc ggcgaggttg cgcacgcgtg
     5401 cgatcggcag gtgctcgatc gccccggccg acgtgtgtga cacgaccggg gtgacctgcg
     5461 ccgtgcgccg ctcgccgatc acgagccccg cggcgcccgc gcactcggcc acgcggcaga
     5521 tcgcgccgag gttgcgcggg tcctggacct ggtcgagggc gaccaccacc gcattctcgt
     5581 ccgcgagcag cccgcccggg tcggcgtact cgaactcgct cacctcggcg cagatcccct
     5641 ggtggtccgg cgagcccgcg cgcgagtcca gctcggcggc gtcggcgacc cgcacgtcga
     5701 tgccgcgcag ccagggctcc cgcgccgcgt tcttcgagac ccacacggga tgcacgcgac
     5761 gcggcccgcg aagggcctcg cgcaccgggt tgcggccgta gacgatcacg cgcgcacgag
     5821 gcgcggcccc tccggcgtgt cgcgcacctc ccagccgcgc tcgcgcagct cgtcgcggcg
     5881 cgcgtcggcg gtggcgaagt cgcgctcggc ccgggcggcc tcgcgctcgg ccagcagccg
     5941 ctcggcgtcc gggtcggggg cctcgtcctc ggcctcgagg agctgctcga ggccgagcag
     6001 ccacagcatg tcgcgcagcg cccccacgcc gatgcgctca ccggcgtcga ggcggcggtt
     6061 cgcctcagcc acccagccga acaactccgc cagggccgcc ggggtgttga agtcgttcgc
     6121 cagcgcgtcg aggaagcggc cctcgtaggg ctcgatcccc tcggtgccca ccgccggatc
     6181 gaggcggcgc accagctccc gcacgcggcg cacgctggcg gcggcctggt cgagggcgga
     6241 cggggagaac tcgatcggct ggcggtagtg gccgccggcg aagtacatga cgagggtgtc
     6301 ggtcccgacc tggtcgaggg cctcgccgag cccgcggatg ttgcccacgg acttcgacat
     6361 cttctcgtcg gcgaagcgga ccatcccgtt gtgcatccac agccgggcga ggggctcgcc
     6421 gcggcccgcc tcggtctgcg cgatctcgtt ctcgtggtgc gggaacacga ggtccgagcc
     6481 gccgccgtgg atctcgaagt cgagcccgag cagctcctcg gccatcgcgg agcactcgat
     6541 gtgccagccc ggccgcccgc ggccccaggg agcgtcccag gcggtgtcct cgccctcctt
     6601 ctccgccttc cagagcgcga agtcgtgggg cgactccttg tcgccgccga cgtcgtcggg
     6661 ctcgccctgg tcggcttcct cgagcgagcg gttcgagagc ttcccgtact ccgcgaagct
     6721 cgcgaccctg aagtacacgt cgccgcgcga ctcgtaggcg tggccgcgat cgaccagcgc
     6781 gccgatcagc tccacgatcg ccggtacggt ctcgctcgcc ctgggctcgt ggtcgggccg
     6841 gccgaggccg agacgggcgg tgtcgcgctc gtagcgctcg gtcatctcgc gagcgagctg
     6901 ctcgctcggc acccccgcct cccgagcggc gtcgtagatc ttgtcattga cgtcggtgac
     6961 gttgatgacg agcgtcgccg tgtagccgcg gtattcgagg aagcgcttca gcagcgagaa
     7021 gacgacgtac ggccgcgcgt tgccgacgtg gatcggcgcg tagaccgtcg ggccgcacgc
     7081 gtagatgtcg acgcggccct cctcgcgggg agcgagctcg cgcagctcgc cgctcagggt
     7141 gtcgcgaatc gcgatcacgg gcgctcgagc gtcgcgaccg ccatcgcggc gatcccctcg
     7201 ccgcggccga tccagcccat cccctcgttc gtcgtgaact tgacccccac ccgggcggcg
     7261 tcgatcccga gcggcgccgc cagcgtctcg cggatccggt cgcgataggg gccgagccgc
     7321 ggcgcgtcgc agaccaccgt cgagtccacg ttcgccaccg cgaagccctg gtcggagacg
     7381 agcgcgcgca cctcgtcgag cagcgcgagc gagtcggcgc cgcggtagcg ctcgtcgctg
     7441 tcagggaagt gcaggccgat gtcgccgagg ctggccgcgc cgagcacggc gtcgatgatc
     7501 gcgtgcgtga gggcgtccgc gtccgagtgg cccgcgagcc cgagctcgga ctcgacgcgc
     7561 acgccgccga ggatcagcgg ccggccctcc acgagccggt gcgagtcgta gccgatgccg
     7621 gtgcgggcgc tcacccgagc ggctccaggc ggcgctcgcg ccgctcgaac accgcgatct
     7681 cccccacgcc ggcggactcg aggaactcga gtgcctggtc gtagcggtag gcgaggtcgc
     7741 ccggcacgtg agcgtccgag gacagcgcga ccggccgtcc ggcctccagg cacatctcga
     7801 ggaacgccgg agcgggatag atctcgccgg cgggcttgcg cagcccggcg gtcgagacct
     7861 cgatggccac gtcggagtcg gcgatgccgt ccatcgcgag atcgtagaaa cggcgcaggt
     7921 ccccatcggg ctgcgggcgc tcgcgccccc agaccttgac cagatccggg tgggccagga
     7981 tgtcgaacat gccgctgcgc gcggcctcgc cgagggtctc gaagtagcgc cgccagacct
     8041 tctcgggatc ggtgtgcgtc catatgtccc actcgccgcg catgtcgact gcggagtcgc
     8101 gcaggaagtg caccgagccg accacgtagt cccagtcgcg ctcctcgagc aggttcgcca
     8161 tgcggtcctc cgcacccggg acgtagtcgg cctcgatgcc gagcgcgagc gaggtccggt
     8221 cgcggacgaa ctcgcagtag gcgtcgaggt cgtcggtggc gctggcctgc cagaacgggt
     8281 ggcgccacac cgacagcgcc tgctcgaagc ggtgcacgtg ctcggagacc ccgagcgcgc
     8341 ccacaccgcg ctcggccgcg acatcgaggt agcgctcggc gttggcggtc gtgaagtact
     8401 ccgcggccgt ggcctccggc tcgtcggggc gcaggtgcac gtggaggtcg gtcagcatcg
     8461 ggccgccagc agcctctcgg cccgctcgag gtcgagcggc gtcgtcacct tcacgttctc
     8521 cggcggcgcc tcgacgatcc gcaccgagcc gccagcggcc tccacgagcg acgcgtcgtc
     8581 ggtcgcggcg gcgagcgtct cggccggggc gtcgagcgcc cggcgcagca cgccggcgtc
     8641 gaagacctgt ggcgtctgga tggcccacag ccgcgagcga tcgagcgtgc gctcgacccg
     8701 catgtcgtcg ccggcctcct tcacagtgtc ggtcacgcgc gcggccgcca ccgcgccgtc
     8761 gaagccctcg tggcgcacgg ccgccacgca gtcgtgcacc agctccggag tcacaaacgg
     8821 gcgcgcggcg tcgtgcacga ccgcgatcgt cgcctcgggc gccgcggcca gggcgttccg
     8881 aaccgactcc gagcgcgagt ctcccccggt cacgcgatcg ggcgcagcgt ccccgtagcc
     8941 cggcggtacg gccacgacca cgcggtcgca gacgcgctcg agcacctcga ggctccactc
     9001 gagcaatggc cggccggcgc agacgacgag cgccttgggg accccctccc caaggcgctc
     9061 gcctcggccg gcggcgggaa cgatgccgac gaccgtcaac ctgtgtctgc gcctaccccg
     9121 ccgcggcggg tgcgccgttg cgctcggcgt gggcgtccga gatgaggctc tcgaggtgct
     9181 cctcggcctc gccctcctcc atgtcgagcg cgtacatcag ctccgacgcg aggatcttct
     9241 tcgcccgtgt gtacatctgc ttctcgccgg tcgagaggcc cttgtccgcc tcgcggatcg
     9301 cgaggttgcg gacgacctcg gccagctcga acacgtcgcc ggtcttgatc ttgtcccggt
     9361 tgtgcttgaa gcgccggttc cagttcttcg gcatctggct gacgtcgtcg cacagcaccg
     9421 caaccacctt gcgcacgtcg tcgtcgccga tgacgcggcg aagcccggcc tttccggcgt
     9481 tctcgcacgg aaccatgacc gtcatgtcgt tgtgcaggat cttgatcgtc aggtactcgc
     9541 gcttctcgcc gaggacctcc ttctgctcct tcttcaggac cttcccggca ccgtggtgcg
     9601 ggtagacgac ctggtcaccg atcttgtagt cgatgtgcgg agcctcgcgc tcgatctcga
     9661 ggtcctcgac gctctggtcc caatcggtct cgctaatggg gccctcccct ctcttcggtg
     9721 tcacgtttgc aagtagcggt ccacgaggtt gatctcctgc aggcggcgaa ggccctcgcg
     9781 gatgtccttc gctcgcaggt ccccgacgcc ctcgacggcc tctagctcgg cgtcggttgc
     9841 ggcgaggatc tcgtccagcc cctcgaacac gccgacgatc gcgccgatca cgagctttgg
     9901 gagcctcggg atccggccga gtatgcggta gccgcgcggc gagacggggt ggtcgagagt
     9961 gttgagcttg cggtcgtagc cgagcagctc ggctaggcgg ccgaagtcca ggaggtcctc
    10021 gtgcgggagg cgcgcgagct ggtcgacgac cagcgcgaac tggtcctcgc tctcctgtgc
    10081 gatgtagtcg tggacgagcg acgccttgtc ggacgcggca ccgatcatcg tctcctcgag
    10141 ttgcatctcg atcagccggc cctcggtgcc gagctcgacg atgtagcgct cgatctcgac
    10201 ggccatacgg gtcacgagct ccgcgcgctg gagcaccgtg agtacgtcgt ggagcgtggc
    10261 gccgccctcg aactcgagcg cggtgagacg ggtcgagacc tgctcgaggc gggtgcggta
    10321 cttctcgagg gtcgcgagcg cctggttggc cttcgcgagg acgaccggga tctcctcgag
    10381 cacgtacttg ccgccgtcga tgtagagcga gacgacgtca cggcgctgcg agaccgcgat
    10441 caccagcgcg ccggtctgct tcgagacgcg ctcggcggta cggtgacggg tgccggtctc
    10501 gagcgaggcg atcgtcgggt ccggcatcag ctgcacgttc gcccagcaga tcttggtggc
    10561 gttggccgac aggatgatcg ccccgtccat cttggcgatc tggtagagca gcgcgggcgt
    10621 gtagtcgacc tcgaccttga ttccgcccga gtagaggaac gagaggtcct cgttgtcgcc
    10681 gatgcagatc agggcgccgg tgcgggcgtg gaggatgtgg tcgatcccct cgcgcagcgc
    10741 cgaacccggc gcgaccatct ccaatgcctt tacgagccgg ggctcttggc ggtgctctag
    10801 gtctacgacc tcgtccccag catgctgcac ctagcccact atacggcaag tcgccgcact
    10861 ttgcggggca ttttcggctc tgtagtgcct atttgcaggg attatgtgcg gttcagcgga
    10921 tcttgaaggt gacgaagttg cggctcagcg ggttgggctt gagaaccttc ttctcggcgt
    10981 tcccggcgcg atcgaccgcg cggatgcggg ccgtccaggt gcccggacgg tggtagcccg
    11041 tacgggtgaa ggtccagctc gtggtgccac gcgcgaccac gtatgtgggg cggcggcagc
    11101 tcaccacgcg accgagccgg ccgctcggct gtaggtagcg gcagaggccg gtcccgccgc
    11161 cctcgcggcg ggcgatggcg accgtcacgc gggcgagggc accgcagccg cgctcccatg
    11221 cgatgccgcg gaagcgcagg gcggtgcggg aggcgtggat cgggtggcgg ccggggcgga
    11281 agctcgaccg cggggcgagc tcgtccgggc atgggttgcg gccgaagcgc ccggccgggg
    11341 tggcggggtc gcgcgccgga gtggcggggt cgagcccggg ccacgcgggg agctcggcct
    11401 cgtcgccgcc gggcgtgtcc ggggtgccga cggacggtgc gtccggcgcc gtgcggggac
    11461 ccgcggaggg ctcgtgcccg gagttcgggg tggagccgcg gccgtcgtcc aggtcgggct
    11521 tcggctgcgg ggtgggctcg gcgccgggct cgggatcggg gccggggccg gggccgggct
    11581 gcggatcggg gtccgggtcg gggtggggat ccggggccgg gccggggccg gcgcccgggt
    11641 cggcatccgg atcgggcttc gggtcgggat cggggtccgg ccccgggtcg ggaccggtgt
    11701 ccgggccggg gccggagtcc ggattcggat cgggatcgag gtctggatcg ggatgcgggt
    11761 tgggatcggg accggtgtcc gggccggggt cggagtccgg gttcggatcg ggatcgaggt
    11821 cgggatcggg atccgggtcg gggtcgggac cggcgcccgg gccggggtcg gcgtccgaat
    11881 ccggatcggg atcgaggtct ggattgggtt ccgggtccgg atccggctgc gggtccggcg
    11941 ccggctcgtc gtcgtcggcg atggtcacgg tcgcgctcat gtcgccgggc gcggcgccgc
    12001 cctgctcgcc gaggtacacg gagaacgtct cgtccggctc gtcgatcgcg tcgtccacga
    12061 tcgggatcgc gacgcggcgc accagctcgc ctggctcgaa cgtcaccacc tgcgaccggc
    12121 gcgtgtagtc gccgggagcc tcggcactgt cgtcgcgagt cgccgcgacc accgccacgc
    12181 gccgccacgt ggcgcgcgac agccgcacct cgacctccac cgtgcccgtg ccctcgtcca
    12241 ccgccgcgtc cgcgacgctc acgagcggca gcgtcgagct gcgcagccac gtgccgctcg
    12301 gctcgtagtc gggcggcgag tacccgtgcg cttcgtccac ctggagcccc tccgcgaccg
    12361 cgaacgcgcc ggtgaccgag gcgtggcgga cgatccggaa ctcgtcggcc ggctcgggca
    12421 ggaacggagc gatcgtggcc acccgcaggg tcccgtcgtg gcgcgcggc
//
LOCUS       DASVPL010000174        13361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_295290, whole genome shotgun sequence.
ACCESSION   DASVPL010000174 DASVPL010000000
VERSION     DASVPL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13361
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295290"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VF517_09055"
     CDS             <1..180
                     /locus_tag="VF517_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053128.1"
                     /translation="LSGLTSPQRAGLSELASAVLTELTPDRATARGICRLCDPDACGH
                     PDHCPVTRAADAAGV"
     gene            complement(205..1458)
                     /locus_tag="VF517_09060"
     CDS             complement(205..1458)
                     /locus_tag="VF517_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8053129.1"
                     /translation="MLAAIAAGAAAPAAHAQGPAGYCEGDGGPAGATTDAEHTVLVGD
                     PPLPRGVRSHRISIDGVETRVQEAGPADARDAVVFVHGHPGSSRDWDDLVAANGKFAR
                     TIAFDMTGYGQSQKAANPFQSTEGAARFIGGALDRLGVDRAVLGLHDFGGVWGLEWAR
                     ANPSRLIGAVLVNSGVYIDWVPHPQAVAWSTPGAGEAEMASTTRSSFTESIQARTPRR
                     LPEAFVNRMYDDYDRATRCAALRYYRSGFENPNRGREHAAALRELRRPALVIWGEDDP
                     YVPSEHAERQREAFPDARVELIPNSGHWPFVDSADRVRELVVPFLRPRFEVTAPRLVA
                     GSKRLAVRVRSTSMLPALRVRARLRPVDRRGRATGVIGLSRRPVTVGGERTLVLRLSR
                     ALRPGRYELVVRARGLATQRVRLTR"
     gene            1590..2147
                     /locus_tag="VF517_09065"
     CDS             1590..2147
                     /locus_tag="VF517_09065"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8053130.1"
                     /translation="MALKTSSYLILGLVRGGMTSGYAIKRFIEEQRMEVFWATTFAQI
                     YPELAQLESDGYLSHREDPHGARQRRAYALTDEGERAFMAWLHEPGIPPMELRDEGLL
                     RLAFADHLPRGEAIELVQRLRRRAEEGEREFQETILPIGEALRAAGLRFPIEIGRMGA
                     GYYAWSIAHLTRLEAELRAEEAPEG"
     gene            complement(2170..2955)
                     /locus_tag="VF517_09070"
     CDS             complement(2170..2955)
                     /locus_tag="VF517_09070"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX8053131.1"
                     /translation="MSEKQNHRGEAFRALHEGDPFVMPNPWDAGSARELEALGFRALA
                     TTSAGFALTLGRRDGEVTLDEVADHVHAVASATSLPLSVDLENGYGERPDEAAAAIAR
                     VAEAGAVGGSIEDWDPAGHLYDLEHAAARVAAAAETARGLGFPFVLTARAENHIRGNP
                     DLDDTIARLRAYEEAGADVLYAPGLRTDEEIRAVCEALSKPVNVLALPGMAIDAIVAA
                     GGQRISVGGSLAFVAYGAMAAAAASLLEGGDLSVLKPAARLHL"
     gene            3011..3829
                     /locus_tag="VF517_09075"
     CDS             3011..3829
                     /locus_tag="VF517_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8053132.1"
                     /translation="MTQTTSSRPARPPAVEARGLIKHYDDVEAVRGVDLRVMPGEVFG
                     FLGPNGAGKSTTVRMLTTLLTITSGEARVCGIDVTSDPDAARRRIGVALQEAGLDPRQ
                     TGRELLVLQGRLFGLGAPDAAERAQHLLTLVDLVDAADRRIKTYSGGMKRRLDLASAL
                     VNEPEVLFLDEPTTGLDPASRLTVWEEVRRINARGTTVFLTTQYLEEADQLCDRLAII
                     DGGKIVREGTPAQLKSDLSERFAGDGEPTLDDVFLDATGRTRDRTAGEVQEVSV"
     gene            3826..4581
                     /locus_tag="VF517_09080"
     CDS             3826..4581
                     /locus_tag="VF517_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8053133.1"
                     /translation="MSAALSLSSRSLREAVRSPDAIIPTLFIPIFFLVVNTGQAAKIF
                     PSGSTPFLKGQGYGAFQLPTTLLLAASFGAAALFLVEEIEGGYFDKLRATPIPRSAIV
                     LGRLAAEGAKCVVIASVILLLALPFGISVASGVLGFLLLVALTAGWGVVYSGFMQLIA
                     LKTRSAAATQSGGLVFFPLLFLTPNFVPRDLLTRPMEVAATFNPVTYLMESLRSLILD
                     DLDWAHIWPGFAVLAGLGVLMLWLNVRTIAKYD"
     gene            4735..8067
                     /locus_tag="VF517_09085"
     CDS             4735..8067
                     /locus_tag="VF517_09085"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053134.1"
                     /translation="MPREPEVDTFGVQSLDSNTAELLGRVNASGNPTQFRVEYDVASS
                     AWCQSGGFAGEPASRTTATPLAEAHTTYQIVSVELHDLTTGTAYCARLAATNKVGSDF
                     GATVRFTAGAPAVQTLVGESTGPTTGNVAGGVNSVGRASTYWARYGPISSEWCQSGGA
                     SGSPLDVTTPQSIPAGDETTQRVTVGLSGLTPETGYCAQLVASNDAGTGTGDQVTLTT
                     DIAPPNLCSATWGGGEGNWTDPNWTFQAPATDANADGYPDADDTVCIVTGGVTLSGAE
                     TVANLRVTGTARLDIEGGLTLDDPGTVAADDGAATVMSGGTIQLTSTVPANSQLVGGS
                     LTNSGVVSALTGAGGTRTLRFASIVNAGGATLESNTTTDIQTPAFTNSGKVEANGSLA
                     TGRHTTLATAAHSLNQNAGTIGGTGTLLLGNGTYHHNGGDVNATVLSAAVESLDLDGS
                     GSATVNGTGAANTLTSDVAAGKTVKVQGGDRAAMLSLAADVTNAGTIELGNVAAGDNG
                     SATLDLGTRTLANSGTLRSSATGPGAGDRAVAGSGRIANAPAGTLDLDYAIALASHVT
                     NAGTVDVADGVTARVSNGDFVQTAGTTALEGELQLPAGKLDLKGGTLAGGGRVGGPLQ
                     NGGGTVSPGGAAPAQLDVTGGYTQGANGTLRIDVDSATPGTGYDTLAAGGAVTLGGAL
                     VVDSTGFTPSDGDELTFVTSAGALSGSFATEDGMSVGGGRGYATVYAAAAPGSAKLIV
                     VEHHALTVSIDGSGAGAVTSQPAGIACGTACTREYAKGTTVTLTATPADGSRFGGWTG
                     AGTEGCTGVTCQVAMTQARGVTATFARERALSVTRTGNGAGTVSGGGIDCGSDCFESH
                     LQGATVTLTATAAPGSQFSGWSGDCSGTATCQFVMSRSRSVTAVFTKIDAGTPQPQPT
                     PSNPNPNPTPTPAPGDGTPGTPDQQSDPEPTKPGGADSGDNTLDATGASETICGLLGN
                     DVINAMGGDDTVFGDLCGVKAKLAGAQASAGGDDTLNGGTGNDTLYGAGGNDKLRGED
                     GNDRLFGGSGKDTLDGGRGNDRLTGGADANRYSGGAGDDTISARNGKVDTIDCGAGRK
                     DSATVDRRDRVKGCEKVKRARK"
     gene            8140..8928
                     /locus_tag="VF517_09090"
     CDS             8140..8928
                     /locus_tag="VF517_09090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8053135.1"
                     /translation="MMRRFTMVTCAVLVAALTGTASASAGERYVALGDSFSSGTGTRT
                     YFDSNCQRSVYAYPSLVDTQRANTDLVFAACSGARTGDVLANQVSSLTTGTRWVTITI
                     GGNDAGFSSVITECAQPSWSSNCNAAIDGAQSYIRNTLPAKLDQVYASIKSRSPSATV
                     IVLSYPRLFMGVDCNAGTWFSSDEMTRLNATADLLRDTTRARVTAAGTNFRFKDAIPG
                     FVGHAVCSSTEWVNGLSNPIGESYHPNRTGHSSGYAPLVRSVMG"
     gene            complement(8930..9706)
                     /locus_tag="VF517_09095"
     CDS             complement(8930..9706)
                     /locus_tag="VF517_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8053136.1"
                     /translation="MNGRLQAVEDPTPGDSEPRLTVEQLASETGMSVRNIRNHQSRGL
                     LPPPEVRARIGYYSDEHVDRLRLIQEMQAQGFKLTAIKRLIGEHGPSVDQFVGLKRAV
                     TAPFETESPEFLTVDDLVERFGPASEKDIAKAQKLGLLVPVGDGLFEAPSPALLRAAE
                     EVLARGVRLASALSVIEKVKRSSETTSKAFVALFLDEVWKSFSEAGQPNDRWPEVTET
                     IERLRPLASEVVLAMFKQTMEAEVEKAFGKALAEQAKRGG"
     gene            9816..11720
                     /locus_tag="VF517_09100"
     CDS             9816..11720
                     /locus_tag="VF517_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053137.1"
                     /translation="MRKLRVLAPLAIVALAVAPPAHAHTAAATPPAGGAALGEVILAS
                     AAALVLTVSLLVLGLGHRSGRVKVLGRAADTIGRWSGTPGWVALPSAVATASLVTAVF
                     GMYWDIALHIGIGRDEGPLANPAHYFILIGLFGIFTAGYLAMVLPKERPSGVAIRITR
                     DWHAPLGGVLICACGAFSLTGFPLDDVWHRMFGQDVTLWGPTHLMLIGGAAMTLVGIA
                     VLAVEGLRANAAAGDPDRMKTWARATMAVALTGGFMLGLSTFQAEFDFGVPQFRFVFQ
                     PILVMLAASVGLVTARIFGGRGAALGAVAFFLVVRGLLSVAIGPVFGETTPHMPLYVV
                     EALVVELVALRVVAEDRPLAFGLASGIGIGTVGLAAEWGWSHVWMPLAWPAELFPEGA
                     LLGFAAAIAGGLLGAWIGARLSVEPRPRDGRLRTAALAGAALVAVIVGFSLNKPPAEG
                     VRAAVALTEVRGGPERTVDATVTLTPRDAADDAEWLNVTAWQGDGLVVSKLERIGPGR
                     YRTEKPVPVHGNWKSLIRLHVGNSLTAVPLFLPEDPAIPAKEVPASARFERPFVSDHE
                     ILQREQKGGSGALTALAYGTVVAIALSLLALLAWGLHRLGSGAGGPVREGRRRRERSG
                     RALRAVSGNA"
     gene            11713..11844
                     /locus_tag="VF517_09105"
     CDS             11713..11844
                     /locus_tag="VF517_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053138.1"
                     /translation="MPDALLPLAHHAVVESLAFFVPVVVIVAVLLVTLVRDRRSEPR"
     gene            11844..12257
                     /locus_tag="VF517_09110"
     CDS             11844..12257
                     /locus_tag="VF517_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053139.1"
                     /translation="MPGLRAILSPVRRLVRAFGTTRRTVAALPDLVEAILVLPTMSEQ
                     LEVVAFQTATLADLRDELVRVQRNTATLPGIDDRLVRVNEALAQVEGNTRAVEQLAEI
                     ALPLHGAALRVGRLSDRFPRSSERASRPARRSPAS"
     gene            complement(12211..12582)
                     /locus_tag="VF517_09115"
     CDS             complement(12211..12582)
                     /locus_tag="VF517_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053140.1"
                     /translation="MWELVRVLHLLAMAFFVGGQLFLAAAVVPVERGSPDPERMRAIA
                     RRFGWGTLGAIAILLVTGVALASKFHQWDDGVLHAKLALVAVVGALIAWHLRRPDLRA
                     LDGAIFVSSLAIVALGVTLAH"
     gene            complement(12601..>13361)
                     /locus_tag="VF517_09120"
     CDS             complement(12601..>13361)
                     /locus_tag="VF517_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX8053141.1"
                     /translation="LGAERLGAVAEGLPAFDSHVGGHTPPAERLRLHAVAVQRAGESV
                     LLALGGEDGDELSTAALLHDAGKLALAQSEPGYNLVSDRPPSERLERERAAHGTDHAA
                     VGAELARRWGLPERLAAAIEAHHSPDARDAAAIVRLADMLAHYEQGAPVDVHEVEAAG
                     AVVGLGREAIGVLLYELPYPVRAGRRPEPCPLSLRELTVLRKLSEGKVYKQIASDEGI
                     ATSTVRNYLHSAYSKLGVVDRAQAVLMAVDRGWI"
BASE COUNT         1830 a         4945 c         4819 g         1767 t
ORIGIN      
        1 ctcagcggtc tgacgagccc gcagcgtgcg ggcctgagcg agctcgcgag cgcggtcctg
       61 accgagctga ccccggaccg ggcgaccgcc aggggcatct gccggctgtg cgacccggac
      121 gcgtgcggcc accccgacca ctgccccgtg acgcgagcgg ccgacgcggc aggcgtgtag
      181 ccgggcgcgc gcggcccggc gcggtcagcg cgtgagccgc acccgctggg tggcgagccc
      241 gcgcgcgcgg acgaccagct cgtagcgtcc gggccgcagc gcgcgcgaga gccgcaggac
      301 gagcgtgcgc tcgccgccga ccgtcaccgg ccgcctgctc agcccgatca cgcccgtggc
      361 gcggccgcgg cggtcgaccg ggcgcagccg cgcgcgcacg cgcagcgccg gcagcatgct
      421 cgtggagcgc accctcacgg cgagccgctt gctgcccgcc acgaggcgcg gcgccgtgac
      481 ctcgaagcgc ggcctcagga acggcacgac gagctcgcgc acccggtcgg cgctgtccac
      541 gaacggccag tggccgctgt tcgggatgag ctcgacgcgc gcgtcgggga acgcctcgcg
      601 ctggcgttcg gcgtgctcgg acgggacgta cgggtcgtcc tccccccaga tcacgagcgc
      661 tgggcgcctg agctcgcgga gcgcggccgc gtgctcgcgg ccgcggttcg ggttctcgaa
      721 cccggagcgg tagtagcgca gggcggcgca gcgggtcgcg cggtcgtagt cgtcgtacat
      781 gcggttgacg aacgcctcgg ggagccgccg cggagtacgg gcctggatcg actcggtgaa
      841 gctcgagcgc gtggtcgacg ccatctccgc ctcgccggcg ccgggggtcg accacgcgac
      901 cgcctgcggg tgcggcaccc agtcgatgta gaccccgctg ttgacgagga cggcgccgat
      961 gagccggctc gggttcgcgc gggcccactc gaggccccag accccgccga agtcgtggag
     1021 gccgagcacc gcgcgatcga cgccgaggcg atcgagggcg ccgccgatga agcgggccgc
     1081 gccctcggtg gactggaacg ggttggcggc cttctgtgac tgcccgtagc ccgtcatgtc
     1141 gaatgcgatc gtccgggcga acttgccgtt cgcggccacg aggtcgtccc agtcgcgcga
     1201 cgagccgggg tgcccgtgca cgaagacgac cgcgtcgcgt gcgtccgcgg ggccggcctc
     1261 ctggacccgc gtctccaccc cgtcgatgct gatccggtgg ctgcggacgc cgcgcggcag
     1321 cggcgggtcg ccgacgagca cggtgtgctc ggcgtccgtc gtggccccgg cgggcccgcc
     1381 gtcgccctcg cagtagccgg ccgggccctg cgcatgcgcc gccggtgccg cggcaccggc
     1441 ggcgatcgcc gccagcaccg ccgcgatccg cgcacctctg gatgggctcc cgatccgcac
     1501 cccgaggatg gaagccgatc ggagcgccgc gcgcaatggg gagccgtcta ccgatcgaca
     1561 tgcggccacg acgcgagaga atcgagcccg tggccctgaa gacgagcagc tacctgatcc
     1621 tcggactcgt ccgtggcggc atgacctccg gctacgcgat caagcgcttc atcgaggagc
     1681 agcggatgga ggtcttctgg gccacgacgt tcgcgcagat ctaccccgag ctggcacagc
     1741 tcgagagcga cgggtacctg agccatcgcg aggacccgca cggcgcccgg cagcggcgtg
     1801 cgtacgcact gaccgacgag ggtgagcgcg cgttcatggc gtggctgcac gagccgggga
     1861 tcccgcccat ggagctgcgc gacgaggggc tgctgcggct cgcgttcgcc gatcacctcc
     1921 cgcgcggtga ggcgatcgag ctggtgcagc gcctgcgccg gcgcgcggag gagggcgagc
     1981 gcgagttcca ggagacgatc ctgccgattg gcgaggcgct gcgggcggcg ggcctgcggt
     2041 tcccgatcga gatcggtcgc atgggcgccg ggtactacgc gtggtcgatc gcgcacctca
     2101 cgcggctcga ggcggaacta cgcgccgagg aggcccccga ggggtagggc gtctctgacg
     2161 gcgccgcggc tagaggtgca gccgcgcggc cggcttcagc accgacaggt ccccgccctc
     2221 gagcaggctc gccgccgcgg ccgccatcgc cccgtacgcg acgaaggcca gcgacccgcc
     2281 gacgctgatc cgctgcccgc cggccgcgac gatcgcgtcg atcgccatgc ccggcagcgc
     2341 gagcacgttc accggcttgg acagcgcctc gcagaccgcg cggatctcct cgtccgtgcg
     2401 caggccgggg gcgtagagga cgtcggctcc cgcctcctcg tacgcgcgca ggcgtgcgat
     2461 cgtgtcgtcg aggtcggggt tgccgcggat gtggttctcg gcccgcgccg tgagcacgaa
     2521 cgggaacccg agcccgcgcg ccgtctcggc ggccgcggcc actcgggcgg ccgcgtgctc
     2581 caggtcgtag aggtggcccg ccgggtccca gtcctcgatc gagccgccga cggcgccggc
     2641 ctcggcgacc cgggcgatcg cggcggccgc ctcgtcggga cgctcgccgt agccgttctc
     2701 gaggtccacc gagagcggca ggctcgtggc ggacgcgacc gcgtggacgt ggtcggccac
     2761 ctcgtccagc gtgacctcgc cgtcgcggcg cccgagcgtg agcgcgaagc cggcgctggt
     2821 ggtcgcgagc gcgcggaagc cgagcgcctc gagctcgcgc gccgagcccg cgtcccaggg
     2881 gttgggcatc acgaacggat cgccctcgtg gagcgcgcgg aacgcctcgc ccctgtggtt
     2941 ctgcttctcg ctcatggggg ggattatcgc cgagcgatga gttttcgcgc gtgcggcggt
     3001 cttaggttcc atgacccaga caacttcgag tagacccgcc cgcccgcccg ccgtcgaggc
     3061 ccgcggcctg atcaagcact acgacgacgt ggaggccgtt cgcggcgtcg acctccgtgt
     3121 gatgcccggg gaggtcttcg gcttcctcgg tccgaacggg gccggcaagt ccaccaccgt
     3181 ccgcatgctc accaccctgc tgacgatcac gtcgggggag gcgcgcgtct gcggcatcga
     3241 cgtgacgagc gacccggacg ccgcacggcg ccggatcggc gtggcgctcc aggaggccgg
     3301 gctcgacccg cgccagaccg gccgcgagct gctcgtgctc cagggccgcc tgttcgggct
     3361 gggcgcgccc gacgccgccg aacgcgcgca gcacctcctg actctcgtgg acctcgtcga
     3421 cgccgccgac cgccggatca agacctactc cggcggcatg aagcgccggc tcgacctcgc
     3481 ctcggccctc gtgaacgagc ccgaggtgct gttcctcgac gagcccacga ccgggctcga
     3541 ccccgccagc cggctgaccg tgtgggagga ggtgcgccgg atcaacgccc gcggcactac
     3601 ggtcttcctc accacgcagt acctggagga ggccgaccag ctctgcgacc ggctcgcgat
     3661 catcgacggc ggcaagatcg tgcgcgaggg cacccccgcc cagctcaaga gcgacctgag
     3721 cgagcgcttc gcgggcgacg gcgagccgac gctggacgac gtgttcctcg acgccaccgg
     3781 ccgtacgcgc gatcgaacgg ccggcgaggt ccaggaggtg tcggtatgag cgcggcgctc
     3841 tcgctctcga gccggtcgct ccgcgaggcc gtgcgctcgc ccgacgcgat catcccgacg
     3901 ctgttcatcc cgatcttctt cctggtcgtc aacaccgggc aggcggcgaa gatcttcccg
     3961 tccgggtcca cgccgttcct gaagggccag ggctacggcg ccttccagct accgacgacg
     4021 ctgctcctgg cggcgtcgtt cggtgccgcg gcgctgttcc tggtcgagga gatcgagggc
     4081 ggctacttcg acaagctgcg cgctacaccg atcccgcggt cggcgatcgt cctcggccgg
     4141 ctggccgcgg agggcgccaa gtgcgtcgtg atcgcgtcgg tgatcctgct gctggcgctg
     4201 ccgttcggga tctcggtggc gagcggggtg ctcgggttcc tgctcctggt cgcgctcacc
     4261 gcgggctggg gggtcgtcta ctccgggttc atgcagctca tcgcgctcaa gacgcgcagc
     4321 gcggcggcca cgcagtcggg cgggctcgtc ttcttcccgc tgctgttcct gaccccgaac
     4381 ttcgtgccgc gcgacctgct cacgcgcccg atggaggtgg ctgcgacctt caacccggtc
     4441 acctacctga tggagtccct gcgctcgctg atcctcgacg acctcgactg ggcgcacatc
     4501 tggcccggct tcgccgtcct agcggggctc ggcgtgctca tgctctggct caacgtgagg
     4561 acgatcgcca agtacgactg agggcgcacg gaggcgccga ggcgggcgcc gcgcggctca
     4621 agttccaccg actggagccg acgagagttc gcaaggactc tcatgctgct ccgcaaagga
     4681 cttctactaa cggcgcttgc gctggccgcg ctccctgcgc ccgcttcggc ggtcgtgcca
     4741 cgcgagcccg aggtcgacac gttcggcgtc cagtcgctcg actcgaacac ggccgagctg
     4801 ctcgggcgcg tcaacgcgtc cggtaacccg acgcagttcc gcgtcgagta cgacgtggcg
     4861 agctcggcct ggtgccagtc gggagggttc gccggcgagc ccgcgagccg gacgaccgcg
     4921 acgccgctcg cggaggcgca caccacctac cagatcgtca gcgtcgagct ccacgacctg
     4981 accaccggca ccgcgtattg cgcgcgcctc gccgcgacga acaaggtggg atccgacttc
     5041 ggggcgaccg ttcgcttcac cgcgggcgcg cccgcggtcc agacgctggt cggggagtcc
     5101 accgggccga ccaccggcaa cgtggccggc ggcgtgaact ccgtcgggcg cgcctcgacg
     5161 tactgggccc gctacggccc gatctcctcg gagtggtgcc agagcggcgg cgccagcgga
     5221 tcccctctcg acgtcacgac cccgcagtcg atccccgcgg gcgacgagac gacgcagcgc
     5281 gtgaccgtgg gcctcagtgg cctcactccc gagaccgggt actgcgcgca gctcgtcgcg
     5341 agcaacgacg ccggcaccgg caccggcgac caggtcacgc tgacgaccga catcgcaccc
     5401 ccgaacctct gcagcgcgac gtggggcggc ggcgagggca actggaccga tcccaactgg
     5461 accttccagg cgcccgcgac cgacgccaac gcggacggct acccggacgc cgacgacacg
     5521 gtgtgcatcg tcaccggcgg cgtgacgctc agcggcgcgg agaccgtcgc caacctgcgg
     5581 gtgacgggca ccgcgcgcct cgacatcgag ggcgggctga ccctcgacga ccccggcacc
     5641 gtcgccgcgg acgacggcgc cgcgacggtg atgagcggcg ggacgatcca gctgaccagc
     5701 acggtcccgg ccaacagcca gctggtcggc ggatccctga cgaacagcgg cgtcgtgagc
     5761 gccctcaccg gcgcgggcgg tacgcgcacg ctccgcttcg ccagcatcgt caacgccggg
     5821 ggcgcgacgc tcgagtcgaa caccacgacc gacatccaga cccccgcgtt cacgaactcc
     5881 ggcaaggtcg aggccaacgg gagcctggcg accggccggc acaccaccct cgcgaccgcg
     5941 gcccactcgc tcaaccagaa cgccgggacg atcggcggca ccggcaccct cctcctcggg
     6001 aacggcacct accaccacaa cggcggcgac gtgaacgcga ccgtcctctc cgccgccgtc
     6061 gagtcgctcg acctcgacgg ctccggctcg gccaccgtga acggcaccgg cgcggccaac
     6121 acgctcacct ccgacgtcgc cgccggcaag acggtgaagg tccagggcgg cgaccgggcc
     6181 gcgatgctct cgctggccgc cgacgtgacg aacgccggga cgatcgagct cggcaacgtc
     6241 gccgcgggcg acaacggctc ggccacgctc gacctcggca ctcgcacgct ggccaactct
     6301 ggcacgctgc gctcgtcggc caccgggccc ggcgccgggg accgggccgt cgcgggcagc
     6361 gggaggatcg ccaacgcccc cgccgggacc ctggatctcg actacgcgat cgccctcgcg
     6421 tcgcacgtga cgaacgccgg cacggtcgac gtcgcggacg gcgtcacggc gcgggtgagc
     6481 aacggtgact tcgtccagac cgccggcacg accgcgctcg agggcgagct ccagctcccc
     6541 gccggaaagc tcgacctcaa gggcggcacc ctcgccggcg gcggccgggt cggcgggccg
     6601 ctccagaacg gcggcggcac ggtgagcccg ggcggggccg cgcccgcgca gctcgacgtg
     6661 acgggcggct acacgcaggg ggcgaacgga acactgcgga tcgacgtcga cagcgccact
     6721 cccggcacgg gctacgacac gctcgccgcg ggcggtgcgg tcaccctcgg cggcgccctg
     6781 gtcgtcgaca gcacgggctt caccccgagc gacggcgacg agctcacgtt cgtcacatcg
     6841 gccggcgcgc tgagcggatc cttcgcgacc gaggacggaa tgagcgtcgg cggcggtcgc
     6901 ggctacgcca ccgtctacgc ggcggcggcg cccgggtcgg cgaagctgat cgtggtcgag
     6961 caccacgctc tcaccgtcag catcgacgga agcggcgccg gcgcggtcac gagccagccc
     7021 gccgggatcg cctgcggcac cgcgtgcacg cgcgagtacg cgaagggcac cacggtcacg
     7081 ctgaccgcga cccccgcgga cggctcgcgc ttcggcggct ggaccggcgc cggcaccgag
     7141 ggctgcacgg gcgtgacctg ccaggtcgcg atgacccagg cgcgcggcgt gaccgccacc
     7201 ttcgcccgcg agcgcgcgct gagcgtgacc aggacgggca acggggcggg caccgtcagc
     7261 ggcggcggca tcgattgcgg ctcggactgc ttcgagagcc acctccaggg cgcgacggtg
     7321 acactcacgg cgaccgcggc ccccggatcg cagttcagcg gctggagcgg cgactgctcg
     7381 gggaccgcga cctgccagtt cgtgatgagc cgctcgcgca gcgtgaccgc ggtcttcacg
     7441 aagatcgacg ccggaacgcc gcagcctcag ccgaccccct cgaacccgaa cccgaacccg
     7501 accccgaccc cggcgcccgg cgacggcaca cccggcaccc ccgaccagca gagcgacccc
     7561 gagcccacga agcccggtgg cgccgactcc ggcgacaaca cgctcgacgc cacgggtgcc
     7621 agcgagacga tctgcggcct gctcgggaac gacgtcatca acgccatggg cggcgacgac
     7681 acggtcttcg gcgacctctg cggcgtcaag gcgaagctgg cgggcgcgca ggcctccgcg
     7741 ggcggcgacg acacgctcaa cggcggcacc ggcaacgaca ccctctacgg cgccggcggc
     7801 aacgacaagc tgcgcggcga ggacgggaac gaccggctct tcggcggcag cggcaaggac
     7861 accctcgacg gcgggcgcgg caacgacagg ctcacgggcg gcgcggacgc gaacaggtac
     7921 agcggcggcg ccggcgacga cacgatcagc gcgaggaacg gcaaggtcga caccatcgac
     7981 tgcggcgcgg gcaggaagga ctccgcgacg gtcgacaggc gcgacagggt caagggctgc
     8041 gagaaggtca agcgcgcgag gaagtaggcc agacgcaccc gcgtcccggc tccgtgcaat
     8101 gatgcccgcg acggggggct ctacgggagg gagcggggga tgatgcgtcg gttcacgatg
     8161 gtgacgtgtg ccgtgttggt ggcggcactg accggaacgg cgtccgcgag tgcgggcgag
     8221 cgctatgtcg cgctgggtga cagcttctct tcgggtacgg gcacgcgcac gtacttcgat
     8281 tcgaattgcc agaggagcgt gtacgcgtac ccgtcgctcg tcgacacgca gcgcgcgaac
     8341 acggacctcg tcttcgccgc gtgctcgggc gcgcggaccg gcgacgtgct cgcgaaccag
     8401 gtgtcgagcc tcacgaccgg gacgcgctgg gtcacgatca cgatcggcgg caacgacgcc
     8461 ggcttctcga gcgtgatcac ggagtgcgcg cagccgtcgt ggtcgtccaa ctgcaacgcg
     8521 gcgatcgacg gcgctcagag ctacatccgg aacacgctgc cggccaagct cgaccaggtg
     8581 tacgcgtcga tcaagtcacg gtccccgtcg gccacggtga tcgtcctgtc ctacccgcgg
     8641 ctgttcatgg gcgtggactg caacgccggg acgtggttca gctcggacga gatgacgcgc
     8701 ctgaacgcca cggccgacct gctgcgcgat acgacgcgcg cccgggtcac cgccgccggg
     8761 acgaacttcc ggttcaagga cgcgatcccg gggttcgtgg ggcacgcggt ctgctcgagc
     8821 acggagtggg tgaacgggct ctcgaacccg atcggcgaga gctaccaccc caaccgcacc
     8881 ggccactcgt cgggctacgc cccgctcgtg cggtccgtca tgggctgagt cagccgccgc
     8941 gcttcgcctg ctccgcgagg gccttgccga aggccttctc gacctctgcc tccatggtct
     9001 gcttgaacat cgcgagcacg acctcggagg cgagggggcg gaggcgctcg atcgtctcgg
     9061 tcacctccgg ccagcggtcg ttgggctgac cggcctcgct gaacgacttc cagacctcgt
     9121 cgaggaacag cgccacgaac gccttggacg tggtctcgga gctgcgcttg accttctcga
     9181 tgaccgacag tgccgacgcg agccgcacgc cgcgggcgag cacctcctcg gccgccctca
     9241 gcagggcagg gctcggtgcc tcgaagagcc cgtcgccgac cggcacgagc aggccgagct
     9301 tctgcgcctt ggcgatgtcc ttctcgctcg cgggcccgaa gcgctccacg aggtcgtcga
     9361 ccgtcaggaa ctcgggcgac tcggtctcga acggagccgt cacggcccgc ttgaggccga
     9421 cgaactgatc gaccgagggg ccgtgctcgc cgatcagccg cttgatcgcc gtgagcttga
     9481 agccctgcgc ctgcatctcc tggatgaggc gcagccggtc cacgtgctcg tcgctgtagt
     9541 agccgatccg cgcgcgcacc tcggggggcg ggagcaggcc gcgcgactgg tggttgcgga
     9601 tgttgcggac cgacatgccg gtctcgctcg cgagctgctc gacggtgagc cgcggctccg
     9661 aatcgccggg cgttggatcc tcgaccgcct ggagtcttcc gttcatttcg cacagaacct
     9721 tacccgttta acgtaacctc gcacgaggta acgtttagga ggtaatgatc ggcggcgcga
     9781 gacgcccgcc gccaccgcga cagggaggca cgaccgtgcg caagctgaga gtcctcgcac
     9841 cgctggccat cgtggcgctc gccgtcgcgc cgcccgccca cgcccacacc gctgcggcca
     9901 ccccgcccgc aggcggggcg gcgctcggcg aggtgatcct cgcgtcggcc gcggcgctcg
     9961 tcctgaccgt cagcctgctc gtcctgggcc tgggccaccg ctcgggccgg gtgaaggtcc
    10021 tcggccgggc cgccgacacg atcgggcgct ggtcgggcac gccggggtgg gtggcgctgc
    10081 cgagcgccgt ggccaccgcg tcgctcgtca cggccgtctt cgggatgtac tgggacatcg
    10141 ccctgcacat cggcatcggg cgcgacgagg ggccgctcgc caatcccgcc cactacttca
    10201 tcctgatcgg cctcttcggg atcttcaccg cgggctacct cgcgatggtg ctcccgaagg
    10261 agcggccgag cggcgtcgcg atccggatca cccgcgactg gcacgcaccg ctcggcggcg
    10321 tcctgatctg cgcgtgcggc gcgttctcgc tgaccggctt cccgctcgac gacgtctggc
    10381 accgcatgtt cggacaggac gtgacgctct gggggccgac gcacctgatg ctcatcggcg
    10441 gcgccgcgat gaccctcgtc ggcatcgccg tgctcgccgt cgagggactg cgcgcgaatg
    10501 ccgccgcggg cgacccggat cgcatgaaga cgtgggcgag ggccaccatg gcggtcgcgc
    10561 tcaccggcgg tttcatgctc ggcctctcca cgttccaggc cgaattcgac ttcggcgtgc
    10621 ctcagttccg cttcgtcttc cagccgatcc tcgtgatgct ggccgcgtcc gtcggcctcg
    10681 tcaccgcgcg gatcttcggg gggcgcggtg ccgcgctcgg cgccgtcgcg ttcttcctcg
    10741 tcgtgcgcgg gctcctgtcg gtcgcgatcg gcccggtgtt cggtgagacc accccgcaca
    10801 tgccgctcta cgtggtcgag gcgctggtgg tcgagctggt cgcgctgcgc gtggtggctg
    10861 aggaccgccc gctcgcgttc ggcctcgctt cgggcatcgg gatcggcacc gtcgggctcg
    10921 ccgccgagtg gggctggtcg cacgtctgga tgccgctggc ctggcccgcg gagctcttcc
    10981 cggagggcgc gctgctcggc ttcgccgcag cgatcgccgg cgggctgctc ggcgcgtgga
    11041 tcggagcgcg gctgtcggtc gagccgcgtc cgcgcgacgg ccggctgcgc acggccgccc
    11101 tcgccggtgc ggccctcgtc gcggtaatcg tcggcttctc gctgaacaag ccgcccgcgg
    11161 agggcgtccg ggcggccgtc gcgctgacgg aggtccgggg cgggcccgag cgcacggtcg
    11221 acgcgaccgt gaccctcaca ccgcgcgacg ccgccgacga cgccgagtgg ctcaacgtca
    11281 ccgcctggca gggcgatggc ctcgtcgtca gcaagctcga gcggatcggc cccggccgct
    11341 accggaccga gaagcccgtg ccggtgcacg gcaactggaa gtcgctcatc cgcctgcacg
    11401 tcggcaactc gctcacggcg gtgccgctgt tcctgccgga ggacccggcg atcccggcga
    11461 aggaggtgcc ggcgagcgcg cggttcgagc gtccgttcgt ctccgaccac gagatcctcc
    11521 agcgcgagca gaagggcggt tcgggcgcgc tgaccgccct cgcctacggc accgtggtcg
    11581 cgatcgcgct gtcgctcctg gccctgctgg cctgggggct ccaccgtctc gggtccgggg
    11641 ccggcgggcc ggtgcgcgag ggccggcgcc ggcgcgagcg ctcgggccgg gcgctgcggg
    11701 cggtgagcgg gaatgcctga cgcgctcctg cccctcgccc accatgcggt ggtcgagtcg
    11761 ctggcgttct tcgtgccggt cgtggtgatc gtcgccgtcc tgctcgtcac gctcgtgcgc
    11821 gaccggcgat cggagccgcg ctgatgccgg gcctgcgcgc gatcctgagc ccggtccgcc
    11881 gcctcgtgcg tgcattcggg acgacgcggc ggacggtggc ggcgctaccc gacctagtcg
    11941 aggcgatcct cgtcctgccc accatgtccg agcagctcga ggtggtcgcc ttccagaccg
    12001 cgacgctcgc cgacctgcgc gacgagctcg tccgcgtcca gcgcaacacc gcgacgctgc
    12061 cgggcatcga cgaccgcctc gtccgggtga acgaggccct ggcgcaggtc gagggcaaca
    12121 cgcgggcggt ggagcagctc gcggagatcg cgctgccgct ccacggtgcg gccctgcggg
    12181 tgggccggct gagcgaccgc tttccgcgct ctagtgagcg agcgtcacgc ccagcgcgac
    12241 gatcgccagc gagctgacga agatcgcccc gtcgagggcg cgcaggtcgg gccggcgcag
    12301 gtgccaggcg atcagcgcgc cgaccacggc gaccagcgcg agcttggcgt gcagcacccc
    12361 gtcgtcccac tggtggaact tgctcgccag ggccaccccg gtgacgagca ggatcgcgat
    12421 cgctccgagc gtcccccagc cgaaccgccg cgcgatggct cgcatgcgct cggggtcggg
    12481 tgagccgcgc tcgaccggca cgaccgcggc cgcgaggaac agctggccgc cgacgaagaa
    12541 cgccatcgcg aggaggtgca gcacgcgaac gagctcccac atcggccctg ctcctcctga
    12601 ctagatccag ccgcggtcga cggccatgag gaccgcctgc gcccggtcga cgacgccgag
    12661 cttcgagtac gcgctgtgaa ggtagttgcg caccgtgctc gtggcgatcc cctcgtcgga
    12721 ggcgatctgc ttgtagacct tgccctcgct cagcttgcgc agcaccgtca gctcgcgcag
    12781 cgacaggggg cacggctccg gccggcggcc cgcgcgcacc ggatacggca gctcgtagag
    12841 gagcaccccg atcgcctcgc ggcccaggcc cacgacagcg cccgccgcct ccacctcgtg
    12901 cacgtccact ggtgcgccct gctcgtagtg cgcgagcatg tcggcgaggc gcacgatcgc
    12961 ggcggcgtcc cgcgcgtcgg gggagtggtg cgcctcgatg gccgcggcca gccgctccgg
    13021 caggccccag cggcgcgcga gctccgcccc gacggccgcg tgatcggtgc cgtgggccgc
    13081 ccgctcgcgc tcgagccgct cggacggagg gcggtcgctc accaggttgt agccgggctc
    13141 gctctgcgcg agcgcgagct tgccggcgtc gtggagcagc gccgcggtgg acagctcgtc
    13201 gccgtcctcg ccaccgagcg cgaggagcac gctctcgccg gcgcgctgga ccgccaccgc
    13261 gtgcagccgc agccgctcgg cgggcggcgt atgcccgccc acgtgcgagt cgaacgccgg
    13321 cagtccctcg gcgaccgcgc cgagccgctc ggcaccgagg a
//
LOCUS       DASVPL010000175         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_295550, whole genome shotgun sequence.
ACCESSION   DASVPL010000175 DASVPL010000000
VERSION     DASVPL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295550"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VF517_09125"
     CDS             complement(<1..1156)
                     /locus_tag="VF517_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053142.1"
                     /translation="MSPRHDRLRAAAAAAAAIGLAAAATAALLVLASLALRPPLEARA
                     ADPGRWTQTGVSTVPLYYYQGISSDPGKRIFFNGVYTGLYRTDPFLNETARRDHVIPP
                     DVSARENYNHIGDIDWDSREGGRVLLPLECYYGNSNTCRNGAIGVADAETLEWRYYVK
                     LDPAEIEKAMWAEVSPDGKLLWTQSGADLLAYSMDDISAANAAPAAAPIKAVRRLPGA
                     RPPSGITGAAFFRGKLFVAGQDNPAGFQVWSIDLATGARELEIEQQIVGESEGIDFFE
                     GLGGSLHWMIQPYNESNVPTYGVANGTVLHFVPKGESPGTPAAKPAKIHVSAQPRHLP
                     RGRSVRVTFTATARVAGRAAPQKGVVVSFLGRSARTNRDGKASLRVRPGRA"
     gene            complement(1191..1943)
                     /locus_tag="VF517_09130"
     CDS             complement(1191..1943)
                     /locus_tag="VF517_09130"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8053143.1"
                     /translation="MSGHSKWASIKHKKAVTDSRRGAQFTKLARAIQVAAREGGGELV
                     GNPALQLAVQKAREASMPKANIVRAIAKGTGADQDADAFERVLYEGYGPGGVAVLVET
                     LTDNRNRTGSEVRHLFSRSGGNLGEPGSVAWVFEKKGVIVVDAQRASEDDLMVAIDAG
                     AEDVNLDENVYEIVTEPSDLQSAREALEGADIELVSAELTMRPTNRVEIADDQVGALM
                     RLLEALDDHDDVQGVHANFEADAAALEKAAAA"
BASE COUNT          254 a          704 c          718 g          316 t
ORIGIN      
        1 cggcacgccc gggcctcacc ctaagtgacg cctttccgtc gcggttggtc ctggcagacc
       61 ggccgaggaa gctcaccacc acgcccttct gcggagcggc gcgtccggcc acacgcgccg
      121 tcgccgtgaa cgtgacgcgc accgaccgac cgcgcgggag gtgccgcggt tgcgcgctca
      181 cgtggatctt ggccggctta gccgcgggag tgcccgggct ctcccccttc ggcacgaagt
      241 gcagcaccgt gccgttcgcc accccgtacg tgggcacgtt cgactcgttg tagggctgga
      301 tcatccagtg gaggctcccg ccgaggccct cgaagaagtc gatcccctcg gactcgccga
      361 cgatctgctg ctcgatctcg agctcgcggg cgccggtggc gaggtcgatc gaccacacct
      421 ggaacccggc cgggttgtcc tgacccgcca cgaacagctt gccgcggaag aaggcggcgc
      481 cggtgatgcc gctcggcggg cgggcgccgg ggagccgcct gaccgccttg atcggcgcgg
      541 cggcgggtgc cgcgttcgcc gcgcttatat cgtccatgct gtaggcgagc aggtcggcgc
      601 cgctctgggt ccacagcagc ttgccgtcgg gcgagacctc ggcccacatg gccttctcga
      661 tctcggcggg gtcgagcttc acgtagtagc gccactcgag cgtctcggcg tccgcgaccc
      721 cgatcgctcc gttgcggcac gtgttgctgt tgccgtagta gcactcgagc gggagcagta
      781 cgcggccgcc ctcgcgcgag tcccagtcga tgtcgccgat gtggttgtag ttctcgcgcg
      841 cgctgacgtc gggcgggatg acgtggtcgc gacgcgcggt ctcgttgagg aacgggtcgg
      901 tccggtagag cccggtgtag acgccgttga agaagatccg cttgcccgga tcggagctga
      961 tcccctggta gtagtagagc ggcaccgtcg agacgccggt ctgggtccag cggcccgggt
     1021 cggcggcgcg ggcctcgagg ggcgggcgga gggcgagcga cgcgagcacg agcagcgccg
     1081 cggtcgcggc ggcggcgagc ccgatcgcgg cggcggccgc agcggcggca cgcaggcgat
     1141 cgtggcgggg gctcaacgcg gcttccgccg gagcgccgcc gcccgactgg tcacgccgcc
     1201 gcggccttct ccagcgcagc ggcgtcggcc tcgaagttcg cgtggacgcc ctggacgtcg
     1261 tcgtggtcgt cgagcgcctc cagcagccgc atcagcgcgc cgacctggtc gtcggcgatc
     1321 tccacgcggt tggtcgggcg catcgtcagc tcggcggaga cgagctcgat gtcggcgccc
     1381 tccagcgcct cgcgcgcgga ttggagatcg ctcggctcgg tcacgatctc gtagacgttc
     1441 tcgtcgaggt tcacgtcctc ggcgcccgcg tcgatcgcga ccatgaggtc gtcctcgctg
     1501 gcccgctgcg catcgaccac gatcacgccc ttcttctcga acacccacgc gaccgacccc
     1561 ggctcgccca ggttgccgcc cgaacgcgag aacaggtgcc gtacctcgga cccggtgcgg
     1621 ttgcggttgt cggtgagggt ctcgacgagc accgccaccc ccccggggcc atagccctcg
     1681 tagaggaccc tctcgaacgc gtcggcgtcc tggtcggcgc ccgtgccctt cgcgatagcg
     1741 cgcacgatgt tggccttcgg catgctcgct tcccgggcct tctggaccgc gagttggagt
     1801 gccgggttgc cgacgagctc gcctccaccc tcgcgcgccg ccacctggat ggcgcgcgcg
     1861 agcttggtga actgcgcgcc gcggcgcgag tccgtgaccg ccttcttgtg cttgatggaa
     1921 gcccacttgg aatgaccgga catgggagcc agtctaggag cggaagccgg gcggcgaagc
     1981 ggcgcgcggg gc
//
LOCUS       DASVPL010000176         5074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_21298, whole genome shotgun sequence.
ACCESSION   DASVPL010000176 DASVPL010000000
VERSION     DASVPL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21298"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VF517_09135"
     CDS             <1..659
                     /locus_tag="VF517_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8053144.1"
                     /translation="VRDDLAFRYLRGEGIEIGAFHMPQRLPPGARARYVDYATEDVLR
                     TLDPMHPWVHPVDVVDEAERLAKFDDATLDFLIANHVLEHTEDPVTALHTFLRVLRPG
                     GILFLTLPDPRGTFDQERERTTVEHVMRDHREGPGTSRRAHYEEWAIHIEGANDERVI
                     AELVERYAAEGTRNHFHVWEPRTFLELLLALELPFDIEAMQVTHPEFSVILRKSPTPD
                     "
     gene            671..1660
                     /gene="moaA"
                     /locus_tag="VF517_09140"
     CDS             671..1660
                     /gene="moaA"
                     /locus_tag="VF517_09140"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX8053145.1"
                     /translation="MAREPLIDGHGRLLGDLRVSVTDRCNFRCQYCMPADGLPWLDRD
                     EVLRFEEIERVVRVLASMGVASVRLTGGEPLVRRELPRLVAMLAPLVEDLSLTTNGYL
                     LERDADALASAGLRRINVSLDTLQRDRFFQLTRRDSLAQVLRGLDAVARRPELRPVKV
                     NAVALRGFSEEEALPFAEFARRTGFEVRFIEFMPLDADRAWSADDVLPGDELRALIGR
                     VHPLEELPRPASSTARVFRFADGRGSIGFVNPVSEPFCADCNRLRLTADGKLRTCLFS
                     LHETDLRPALRGDASDAELEQIVRDAAWRKELKHHVGEPGFRQPVRTMSAIGG"
     gene            1770..2660
                     /locus_tag="VF517_09145"
     CDS             1770..2660
                     /locus_tag="VF517_09145"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX8053146.1"
                     /translation="MQLAELAARQHGVVARWQLYVLGFRRSAIQRRLESGRLHRVHRG
                     VYAVGHTKLTERGRWIAAVLAFGPDALLSHRSCAALRSLLPDSRAVIDVTVPGRTRRG
                     LAGVRLHLPRRLHPDDVDVHDGIPCTSVARMLLDVAETAPARQLEKVFEAAERERVLD
                     MRRVSEAMGRANGHRGRQPLVTLSEQFHELPPTIRSRLERRFLRLCDEAGLPRPAMNV
                     SVLGHEVDMLWADAKVIVELDSWRFHGTRQAFERDRRRDIDLQLAGHRILRFTWRRLQ
                     TEPHSLIAAVTRAIELASPA"
     gene            2657..3016
                     /locus_tag="VF517_09150"
     CDS             2657..3016
                     /locus_tag="VF517_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053147.1"
                     /translation="MSGWTVKVRVGPRVERTRHEELKDALREVQRRGKELEAGADAKP
                     RSPLGRTFEPVQQVVARIELSGPNRTRAGVDVRGDGSAEAYSGRLRRVPLAQRQGESP
                     YDALRRSLGADKRRGPR"
     gene            3013..3321
                     /locus_tag="VF517_09155"
     CDS             3013..3321
                     /locus_tag="VF517_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053148.1"
                     /translation="MSRRWLVPLPPGAEPPYRVFVNGVPQTEGDDYEVRGDTLLFARE
                     LRKEGRIGFWRWTLMFFSIMGTYRKNDSVDVEYSHAGGRRLVVGLEIVQPEGESASGS
                     "
     gene            3331..4227
                     /gene="metF"
                     /locus_tag="VF517_09160"
     CDS             3331..4227
                     /gene="metF"
                     /locus_tag="VF517_09160"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX8053149.1"
                     /translation="MRIEEILEERRPVFSFEFFPPKTEEGEQNLWSALEELKRDEPAF
                     VSVTYGAGGSTRDRTLGIVKRIKSELGLEAMAHFTCVGATVEDLRGTLDEMRDAGLEN
                     VLALRGDPPEGQEEWRRTEGGLEYSTELARLIADNYDFSIGAACFPEVHPEAADMESD
                     ISFLKKKVEAGARFLITQLFFDNEMYFDFVREARAAGIDVPIIPGIIPVTGYAQIKRF
                     TQRCGASIPDLFERELAARQDDPDAVKDLGVAYATLQCADLLARGAPGIHFFTLNRSP
                     ATRSILAALRAARPWDRVAEAV"
     gene            complement(4224..>5074)
                     /locus_tag="VF517_09165"
     CDS             complement(4224..>5074)
                     /locus_tag="VF517_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8053150.1"
                     /translation="TGLDEPGALRQLELALDQAGLRLDHVRLLVCTHAHSDHYGLAGP
                     IVDAAGCELWMHPDHAHMTARAEDPDAMLDRRMEVARQSGVPEDIVRGYESRRGESIG
                     IDRLVMPDRDLLPGVEVETDHGVWQVHETPGHAPSHVCLHQPESGLLLSGDHLLGRVS
                     LYYDWGYSPDPAGEFLSSLDVVDRLDARLCLAGHARPFRDVRAHIDANRSAIAERVDR
                     VRGALAGGAKTPFEIVPTLMGTEELTPILLNWGLNETLCYLRYLELRDEVEKVDAEPE
                     RWAAAA"
BASE COUNT          720 a         1803 c         1808 g          743 t
ORIGIN      
        1 ccgtgcgcga cgacctcgcg ttccgctacc tacgcggcga gggcatcgag atcggcgcct
       61 ttcacatgcc gcagcgcctg ccgcccggtg cccgcgcgcg ctacgtggac tacgcgaccg
      121 aggacgtgct gcgcacgctc gacccgatgc atccgtgggt ccacccggtg gacgtcgtgg
      181 acgaggccga gcggctcgcc aagttcgatg acgcgacgct cgacttcctg atcgccaacc
      241 acgtgctcga gcacaccgag gacccggtga ctgcgctgca cacgttcctg cgcgtgctgc
      301 ggccgggcgg catcctcttc ctcacgcttc ccgacccgcg cgggacgttc gaccaggagc
      361 gcgagcgcac caccgtcgag cacgtgatgc gagaccaccg cgaggggccg gggacctccc
      421 ggcgcgcgca ctacgaggag tgggcgatcc acatcgaggg cgcgaatgac gagcgggtca
      481 tcgccgaact ggtggagcgc tacgccgccg aggggacccg caaccacttc cacgtctggg
      541 agccgaggac gttcctcgag ctcctgctcg cgctcgagct gccgttcgac atcgaggcga
      601 tgcaggtgac ccacccggag ttctcggtca tcctgcgcaa gagccccacg ccggactagc
      661 cttcggggtc gtggcgcgcg aaccgctcat cgacgggcac gggcggttgc tcggcgacct
      721 gcgcgtctcg gtcaccgacc gctgcaactt ccgctgccag tactgcatgc cggcggacgg
      781 gctgccgtgg ctcgatcgcg acgaggtgct gcggttcgag gagatcgagc gggtcgtgcg
      841 cgtgctcgcg tcgatggggg tcgcgtcggt gcgtttgacc ggcggggagc ccctggtccg
      901 gcgcgagctg ccgcgtttgg tcgccatgct cgctccgctc gtcgaggatc tctcgctgac
      961 tacgaacggc tatctattgg aacgagatgc ggatgcgctg gcttcggctg gcctgcggcg
     1021 catcaacgtc tccctggaca cccttcagcg cgaccggttc ttccagctca cgcggcgcga
     1081 ctcgctcgcg caggtcctgc gggggctcga cgccgtggcg cggcggcccg agctgcgacc
     1141 cgtgaaggtc aacgcggtgg cgctgcgtgg cttcagcgag gaggaggcgc tgccgttcgc
     1201 ggagttcgcg cgccggaccg ggttcgaggt gcgcttcatc gagttcatgc cgctcgacgc
     1261 cgaccgcgcg tggtcggctg atgacgtgct gccgggggat gaactgcggg cactcatagg
     1321 gagggtccac ccgctggagg agcttccgcg tccggcgtcc tcgaccgcgc gcgtctttcg
     1381 cttcgccgac ggccgcgggt cgatcgggtt cgtcaatcct gtctccgagc cgttctgcgc
     1441 ggactgcaac cgcctgcgcc tcaccgccga cggcaagctg cgcacgtgcc tcttctcgct
     1501 gcacgagacc gacctgcgcc cggcgctgcg cggcgacgcc tccgacgccg agctcgagca
     1561 gatcgtgcgc gacgccgcct ggcgcaagga gctcaagcac cacgtgggcg agccgggctt
     1621 ccgccagccc gtgcgcacga tgagcgccat cggcggctga gtcgtcacat aggtaaccca
     1681 cacgtaacgc gccccgtggc agcctgggcc gcatgggcta cctggtcgat tctgtacatc
     1741 aaatacgggc cttagatcga ccagttgacg tgcagctcgc cgagctggcg gcccgtcagc
     1801 acggcgtggt cgcgcgctgg cagctctatg tgctcggttt tcggcgcagc gcgatccagc
     1861 gacggttgga gagcggccgg ctgcaccgcg tgcaccgcgg cgtctacgcg gtcgggcaca
     1921 ccaagctcac cgagcgcggt cgctggattg ccgccgtgct cgccttcggc cccgacgcgc
     1981 tgctgagcca ccgcagctgc gccgcgcttc ggagcctcct tcccgactcg cgcgcggtga
     2041 tcgacgtgac cgttccgggg cggacgcgga gggggctcgc cggcgtgcgg ctgcacctgc
     2101 cgcgcaggct ccaccccgac gacgtcgacg tgcatgacgg catcccgtgc acctcggtcg
     2161 cgcggatgct gctcgacgtg gctgagaccg ccccggcccg ccaactcgag aaggtcttcg
     2221 aggccgccga gcgcgagcgg gtgctcgaca tgcgcagggt gagcgaggca atggggcgag
     2281 cgaacggcca cagggggcgc caacctctcg tgaccctgag tgagcagttc cacgagctgc
     2341 cgcccaccat ccgcagccgg ctcgagcgcc ggttcctgag gctctgcgac gaggccgggc
     2401 tcccgcggcc ggcgatgaac gtcagcgtcc tcgggcacga ggtcgacatg ctgtgggccg
     2461 acgcgaaggt gatcgtcgag ctcgacagct ggcgctttca cgggacccgt caggcgttcg
     2521 agcgcgaccg aagacgcgac atcgacctcc agctcgcggg ccacaggatc ctgcgcttca
     2581 cgtggagacg cctgcagacc gagccccatt ccctcattgc tgccgttaca agggcgatcg
     2641 agctagcttc accggcgtga gcggatggac cgtcaaggtg cgcgtggggc cgcgcgtgga
     2701 gcgcacgcgt cacgaggagc tgaaggacgc gctccgcgag gtccagcggc gcggcaagga
     2761 gctcgaggca ggcgccgacg ccaagccgcg ctcgccgctc gggcgcacgt tcgagccggt
     2821 gcagcaggtg gtggcgcgga tcgagctctc cggcccgaac cgcacccgcg cgggcgtgga
     2881 cgtgcgcggc gacggctccg ccgaggccta ctccggccgc ctgcgccggg tgccgctcgc
     2941 gcagcgccag ggcgagtcgc cctacgacgc cctgcgccgc tcgctcggcg cggacaagcg
     3001 gcggggcccg cggtgagccg ccgctggctt gtgccgctcc cgccgggtgc cgagccgccc
     3061 taccgcgtgt tcgtgaacgg cgtgccgcag accgagggcg acgactacga ggtgcgcggg
     3121 gacaccctgc tgttcgcgcg cgagctgagg aaggaggggc ggatcggctt ctggcgctgg
     3181 acgctgatgt tcttctcgat catgggcacg taccgcaaga acgactccgt cgacgtcgag
     3241 tactcgcacg ccggagggag gcgcctggtg gtcgggttgg agatcgtgca gccggaaggg
     3301 gagtcggcct ccggctcgta acctctaggc atgcgcatcg aagagatcct cgaagagcgc
     3361 cgcccagtct tctccttcga gttcttcccc ccgaagaccg aggagggcga gcagaacctg
     3421 tggtcggcgc tggaagagct gaagcgcgac gagcccgcgt tcgtctcggt gacctacggg
     3481 gccggcggct ccacccgcga ccgcacgctc ggcatcgtca agcggatcaa gagcgagctc
     3541 gggctcgagg cgatggcgca cttcacctgc gtgggcgcca ccgtggagga cctgcgcggc
     3601 accctcgacg agatgcgcga cgcgggcctg gagaacgtgc tcgccctgcg cggcgacccg
     3661 ccggagggcc aggaggagtg gcgcaggacc gagggcggcc tcgagtactc caccgagctg
     3721 gcccggctca tcgccgacaa ctacgacttc agcatcggcg ccgcgtgctt cccggaggtg
     3781 caccccgagg ccgcggacat ggagtccgac atctcgttcc tgaagaagaa ggtggaggcc
     3841 ggtgcgcgct tcctgatcac gcagctcttc ttcgacaacg agatgtactt cgacttcgtg
     3901 cgcgaggcgc gcgccgccgg gatcgacgtg ccgatcatcc ccgggatcat ccccgtgacc
     3961 ggctacgcgc agatcaagcg cttcacccag cgctgcggcg cgagcatccc cgacctcttc
     4021 gagcgcgagc tggccgcccg ccaggacgat ccggacgcgg tcaaggacct cggcgtcgcg
     4081 tacgccacgc tccagtgcgc cgacctgctc gcccgcggcg cccccggcat ccacttcttc
     4141 acgctcaacc gctcacccgc gacgcgctcg atcctcgcgg ccctgcgcgc ggcccgcccc
     4201 tgggaccgcg tggccgaagc ggtctaggcc gccgccgccc agcgctcggg ctcggcgtcg
     4261 accttctcca cctcgtcgcg cagctcgagg tagcgcaggt agcagagggt ctcgttcagg
     4321 ccccagttga ggaggatcgg cgtgagctcc tcggtgccca taagcgtggg gacgatctcg
     4381 aacggtgtct tcgcgccccc ggccagcgct ccccgcacgc ggtcgacgcg ctcggcgatc
     4441 gcgctgcggt tggcatcgat gtgagcgcgc acgtcgcgga acggccgcgc gtggccggcg
     4501 aggcagaggc gcgcgtcgag gcggtcgacc acgtcgaggc tcgacaggaa ctcgccggcc
     4561 gggtcggggc tgtagcccca gtcgtagtag agcgagacgc gcccgagcag gtggtcgccg
     4621 gacaggagca gaccgctctc gggctggtgc aggcacacgt gcgagggcgc gtgccccggc
     4681 gtctcgtgca cctgccagac gccgtgatcg gtctcgacct cgacgcccgg gagcaggtcg
     4741 cggtccggca tcaccagccg gtcgatgccg atcgactcgc cgcggcgcga ctcgtacccg
     4801 cgcacgatgt cctccggcac gcccgactgc cgcgccacct ccatccgccg gtcgagcatc
     4861 gcgtccgggt cctcggcccg cgcggtcatg tgcgcgtggt cggggtgcat ccacagctcg
     4921 cagccggcgg cgtcgacgat cggcccggcg aggccgtagt ggtcggagtg cgcatgggtg
     4981 cacacgagca ggcgaacgtg gtcgagccgc aggccggcct ggtcgagggc cagctcgagc
     5041 tggcgcagcg cacccggctc gtcgaggccc gtgt
//
LOCUS       DASVPL010000177        11380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_100878, whole genome shotgun sequence.
ACCESSION   DASVPL010000177 DASVPL010000000
VERSION     DASVPL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11380
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100878"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF517_09170"
     CDS             complement(<1..190)
                     /locus_tag="VF517_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX8053151.1"
                     /translation="MSFAEPLVLLGLIALPLLVGWYLAEQRRRRAAAVAFAAPALHPS
                     VAPVRPRWRRHVPLAIVAL"
     gene            complement(192..1475)
                     /locus_tag="VF517_09175"
     CDS             complement(192..1475)
                     /locus_tag="VF517_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8053152.1"
                     /translation="MTPVIGRPPSDDPPPRGARRIWTGDWLSESERARRAAEEAAARL
                     ETEVAERESARVVRDREVEPEYAYAAADARVPRRGARRRRLLSLPGALAIGLVMAVLA
                     FGAGFLLVGDDTPDPLPAVSGDPIKPHKGQTRAGAVYAAASPAVVSIRAGDGQGTGFL
                     TDAKGQIVTNNHVVGASKRVTVKFGDGASLQADVLGADPSTDLAVLSVEAGRLPKGVK
                     PLKFADSRSVQVGDLAIAIGNPFGLDRTATEGIVSGLGRSIEAPNGFSIDQVIQTDAP
                     INPGNSGGPLLNDAAQVIGVNSQIATAGSQGNVGVGFAVPSNTVRQVVPRLARGEKIN
                     RGYLGVETSDALDGGDGAEVQNVVPGGPAERAGLRPGDIIKKVAGTDVKEPAAVAAAI
                     ETRAPGDKIEVEVERGGGTKKIDVTLGERPAKTGP"
     gene            complement(1538..2299)
                     /locus_tag="VF517_09180"
     CDS             complement(1538..2299)
                     /locus_tag="VF517_09180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8053153.1"
                     /translation="MSTAEYDVRLPKAGETSFDQDQEWCEILIDGEPRRIRFHDYDEI
                     YSIPGLYEHLFYELLECDSPRAVTALLGQELRDRGREAGDLRVLEVGAGNGMVAAELV
                     ELGVRSIVGVDIIEEAAMAAERDRPGIYDDYKILDLTAIPADTGRELQGHGFNCMVTV
                     AALGFGDIPPDAFANAYNFVEPGGLVAFNIKEDFLADADGSGFSKLIQAGLRDGALEI
                     RAEERYRHRLSIAGEPLHYVAIIGEKRSDLPVTGA"
     gene            complement(2296..3318)
                     /locus_tag="VF517_09185"
     CDS             complement(2296..3318)
                     /locus_tag="VF517_09185"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8053154.1"
                     /translation="MQRLVIADVLAGRDVLAQSPTGSGKTLAFGIPLLDSIESQARRP
                     AALILAPTRELASQIVEELRDLATARALSVAAVYGGVGIQKQAKAAARAHLLVATPGR
                     LEDLLERGAVTLKHVQVLVLDEADRMLDMGFKPAVDRIVAKTPRDRQTLFFSATLEGE
                     AGQIARSYTRDARRHVHAAPAKTKADVEHRFVQVARDGRIDALVGELRGGDRTLVFVR
                     TKRGADRLVKRLRGHDVQAVAMHGDKSQSQREKALARFASGGVSTLVATDVAARGIDV
                     DDVCRVINFDMPADRDAYVHRVGRTGRAGRSGSGVSFVMADQAKEMRRIAVDLGLRRE
                     FDRGIA"
     gene            3584..5026
                     /locus_tag="VF517_09190"
     CDS             3584..5026
                     /locus_tag="VF517_09190"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HEX8053155.1"
                     /translation="MVALLAASPAAAQPVLRLDGDRVELRDDPHLPALDLPSPPVSGM
                     RSAGVRRAQVKRTVRGELARLLAEGQIDQATHDSHRSTYNLARKSYKQLTGARKAGLG
                     GALENAELVAASGQLTPSRLAPLFLTVARNRQWWTTGPLLSNGQRVEFAGSELVWQHY
                     TGQGIQLQVLANFGKANGLWQAKDDDALRALLDEMLPLASDRDGILAWEYWFRFGGGS
                     PPWASGMAQATGVQALARAAVQLGEPAYRDAAIRALALFETPAPRGVRVDGEAGPHYL
                     LYSFAPGLRVLNGFLQAVIGLHDAAELLAEPRAQALFEAGDTQARRETQLSDTGAWSL
                     YSTGDDPAAGRESSLHYHRVVTDFLERLCDRVAAGEYCVAADHFKAYEDIAPVVRPLT
                     RRVRAGKPANLRFVLSKVARVRLQVADGDRVVATRTATVGYGKRSLAWTPRSPGTYEL
                     RATAADLAGNDSVERAIELEVLPPRPPKRR"
     gene            5127..5801
                     /locus_tag="VF517_09195"
     CDS             5127..5801
                     /locus_tag="VF517_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053156.1"
                     /translation="MWLAAGTAGLLALGATACGGSESSSSSMMDPVTTALQTPGVRTV
                     QIAQQKFDLEVIVPPCPRPGEKASPNPQSEQDVQTGRVVPPEGSNRIIIPRETLTQTV
                     AVQPCQVDSSGDSSSSSSSSSSSSSSSSAATGQTVSTVLLTPGGLAPQGAAIQEQAGQ
                     AQNQLVLPSNANIQTLVIPPCTQPEQSSSSSSSAAEPPQKNLVLPADSRTKSATAPGC
                     TVQQSG"
     gene            complement(5798..6406)
                     /locus_tag="VF517_09200"
     CDS             complement(5798..6406)
                     /locus_tag="VF517_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8053157.1"
                     /translation="MGTPAFTRLDVDERRRQLLEAGARAFTERAYDEVAMSDVAKAAG
                     ISKGLLYHYFPSKRDFFTATLAAAGAEVAAVTEPDPDRPPVEQLAGSLGAYLAWIEEN
                     ASGYRRLLESAGSPDVRSLIEDVRERTVVRIVEAISEGAPRPALRLAVRAWLWGMDGA
                     ILDWLDRGEASRDELRDLLVATFVGALGAAGHVDPRVKLAVG"
     gene            6561..9290
                     /locus_tag="VF517_09205"
     CDS             6561..9290
                     /locus_tag="VF517_09205"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX8053158.1"
                     /translation="MDVLELVRGHGRRLDAVEREMAGMSERLARLEGVPAERPQAERP
                     PRPAPAGAERPAPRSTRPEGSVREWLDPSTWNVPPTAPPAAGQAPPPTGPPQQPARPA
                     ARPSQRPGGMPARPAAGPTRPAQPAAPRIDLEELLGGRILGLAGALAVLLGVAFLVAM
                     AVDRGWVDEPTRIAIGFGGCSALLLGAVALYERRGRTQAALAAAGAAVAGLFATLTAG
                     AQLYELYPVPVSLAIAAAIGGAATALALRWNARTFAALGLVGALLAPVLVGADQSGLT
                     IAFVLFVLTIAVAVADRRGWSEWLPLTASIVAAPQILDWLVDGPDAVPTVLVLSCFWA
                     ANAVAAASLERTRGGSTMHPIAVILLGGASALVAPTGFAALDELGLHDQAVAWLGALG
                     AAHAGLGLAAIGTRRASRDLAHLSIAIGIGLADVTFALAVEGFALAIGWAASAAALTA
                     LARVRHAAAGSPAADAGNGGDAKRDPAAGETVRLAAGDTARLTVAAAVQIGLALAHAL
                     TLDADPTQLSGRPEDMLAGVAALVACGLAALVSARLLARDPLVRHGGHAIAIATLAYL
                     SAFVIDGQFLAVAWAVEAVAVMALARRARTGVDPVGSISSGALVGLALTHAITIDAQP
                     GELAGPPHELLAGVASLGAVGLAALLAAQLLPRTGPGRAAGHAVTLLVLAYLSAFVID
                     GPLLAAAWAVEAVAILALARHRDGGIDAVGSIGAVSLLAFALLHGLVLEAAPRALVYG
                     VEDLLDAAIALATLAAAALAMARLIPEERRDERLGFVSLAAAALVYLGSVAIVTAFQP
                     TTEGFDTAVGLLDERQQGQLLLSAFWALAGLGALIAGLARRQRAIRVGGFVLLGIATA
                     KVFMFDLSTLDSLYRVGSFVALGLLLLGAAFAWQRTRALRDGAGAPNGGEAGA"
     gene            9287..9673
                     /locus_tag="VF517_09210"
     CDS             9287..9673
                     /locus_tag="VF517_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053159.1"
                     /translation="MSGPESPGTAWPIAPQRPAPPDARPSRRARTLAIIGTVAALLAS
                     LVLGFYALLFDAFACFESCVEGSGDWRDDVDAWQYDVELGLAGAGVVLAVVSVILAST
                     GRLRPAGYAVAAAAVTFAGWWTFVTS"
     gene            complement(9666..10580)
                     /locus_tag="VF517_09215"
     CDS             complement(9666..10580)
                     /locus_tag="VF517_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX8053160.1"
                     /translation="MRLRRDRLRLRHARDRVALLHVTEPHLHDPQTLYRHWEDAQWNP
                     FAIDLARDREQWPGMGEANRALVLFVLGSLMVAEERITTKFSGLVAAYGSEEEATFLA
                     TQQVDEARHMQFYARFQDEVVAAPATVAAHVERAREQVSDSFRVIFDEQLVHAHERLV
                     AAPADRAAKVRFVTIYHQILEATLGLTTFEFATRYLEREGLLPGFVEGYTLIHHDEHR
                     HIGYGTWYLREAVRDDPAAAAVVRETLRDLLPAVAGSLETPDGTDAGVLGVTTEELRE
                     FALRGLTRRMKIIGVPLESVWEPAAELS"
     gene            complement(10514..10996)
                     /locus_tag="VF517_09220"
     CDS             complement(10514..10996)
                     /locus_tag="VF517_09220"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX8053161.1"
                     /translation="MAVLRPHLALTVTDVERSIPFYEALFGAAPEKVRSGYAKFSLAE
                     PALNFTLNEGERESLGAFNHAGVQVASTDDVLAARERLVAAGLAAFDEMDTTCCYARQ
                     DKIWAHDPDGTPWEVFATHEDAEEAGESRRAEADAAACGCGETGCGCATRETASLCCT
                     "
     gene            complement(10984..11337)
                     /locus_tag="VF517_09225"
     CDS             complement(10984..11337)
                     /locus_tag="VF517_09225"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8053162.1"
                     /translation="MTVDLTLAPKQKRPAGATCCAPVARPDVSAAEAERLAAVAKALS
                     DPIRVQLVDVLARHAGEVCVCELVPLFDVTQQTLSHHLKKLREAGLVGVERRGLWAYY
                     YVRPEALEVLKRWLS"
BASE COUNT         1436 a         4263 c         4096 g         1585 t
ORIGIN      
        1 acagcgcgac gatcgccagc ggcacgtgcc ggcgccagcg cgggcgcacc ggtgcgaccg
       61 acgggtgcag cgcgggtgcc gcgaacgcca ccgcggccgc gcgccggcgg cgctgctcgg
      121 cgaggtacca gccgaccagc agcggcagcg cgataagacc gagcaggacc aggggctcgg
      181 cgaagctcat ctcagggacc ggtcttggcc gggcgctcgc ccagcgtcac gtcgatcttc
      241 ttggtccccc cgccgcgttc cacctccacc tcgatcttgt ccccgggtgc gcgcgtctcg
      301 atggcggccg ccaccgcggc gggctccttc acgtccgtgc cggcgacctt cttgatgatg
      361 tcgccgggcc gcaggccggc gcgctcggcc ggaccgcccg ggaccacgtt ctggacctcc
      421 gcgccgtcgc cgccgtctag cgcgtccgag gtctccacgc ccaggtagcc gcggttgatc
      481 ttctccccgc gcgcgagccg gggcaccacc tggcgcacgg tgttcgacgg caccgcgaag
      541 cccacgccca cgttgccctg cgagcccgcc gtggcgatct gcgagttgac gccgatcacc
      601 tgggccgcgt cgttgagcag cgggccgccg gagttgccgg ggttgatcgg ggcgtcggtc
      661 tggatgacct ggtcgatcga gaagccgttc ggcgcctcga tggagcggcc gagccccgac
      721 acgatgccct cggtcgcggt ccggtcgagc ccgaagggat tgccgatcgc gatcgcgagg
      781 tcgccgacct ggacgctgcg cgagtcggcg aacttcagcg gcttcacgcc cttcggcagc
      841 cgtcccgcct cgacgctgag cacggccagg tcggtcgagg ggtcggcgcc gagcacgtcg
      901 gcctggagcg aggcgccgtc gccgaacttg acggtcacgc gcttgctcgc gcccacgacg
      961 tggttgttgg tcacgatctg gcccttggcg tcggtgagga agccggtgcc ctggccgtcg
     1021 ccggcgcgga tcgagaccac ggcggggctg gcggccgcgt acacggcgcc cgcgcgggtc
     1081 tgacccttgt gcggcttgat cgggtcgccg gatacggccg gcagcgggtc cggggtgtcg
     1141 tcgccgacga gcaggaagcc ggcgccgaac gccagcaccg ccatcacgag accgatcgcg
     1201 agtgctccgg ggagcgacag caggcgccgc ctgcgcgcgc cgcgcctagg cacccgcgcg
     1261 tcggccgcgg cgtacgcgta ctccggctcg acctcgcggt cgcggaccac ccgggcgctc
     1321 tcgcgctcgg ccacctcggt ctcgagccgc gcggcggcct cctccgcggc acgccgcgcg
     1381 cgctcggact cggagagcca gtctccggtc cagatgcgcc tcgcgccgcg cggaggcgga
     1441 tcgtcgctgg ggggcctgcc gatcacgggg gtcattcgag cagcgctacc tcaacgcctc
     1501 gtgaagcgcg cgtaagacca gggtaagacc ggcggcgcta cgcgcccgtg accgggaggt
     1561 ccgaccgctt ctccccgatg atcgcgacgt agtgcagcgg ctcgccggcg atcgagagcc
     1621 ggtggcggta gcgctcctcg gcccggatct ccagcgcgcc gtcgcgcagg ccggcctgga
     1681 tcagcttgga gaacccgctc ccgtcggcgt cggcgaggaa gtcctccttg atgttgaagg
     1741 ccaccaggcc gccgggctcg acgaagttgt aggcgttcgc gaacgcgtcg ggcgggatgt
     1801 cgccgaagcc gagcgcggcc acggtgacca tgcagttgaa gccgtggccc tggagctcgc
     1861 ggccggtgtc ggcggggatc gcggtcaggt cgaggatctt gtagtcgtcg tagatccccg
     1921 gccggtcgcg ctcggcggcc atcgcggcct cctcgatgat gtccacgccg actatcgacc
     1981 gcaccccgag ctcgacgagc tccgcggcga ccatgccgtt gcccgcgccc acctcgagca
     2041 cgcgcaggtc accggcctcg cgaccgcggt cgcgcagctc ctgcccgagc agggcggtca
     2101 cggcacgcgg cgagtcgcac tcgagcagct cgtagaagag gtgctcgtag aggcccggga
     2161 tcgagtagat ctcgtcgtag tcgtggaacc ggatccggcg tggctcgccg tcgatcagga
     2221 tctcgcacca ctcctggtcc tggtcgaaac tcgtctcgcc cgccttcggc aggcgtacgt
     2281 cgtattcggc tgtgctcatg cgatccccct gtcgaactcg cggcgcaggc ccagatcgac
     2341 ggcgatgcgc cgcatttcct tggcctggtc cgccatgacg aagctcacgc ccgacccgct
     2401 gcggccggcg cggcccgtgc ggccgacgcg atgcacgtac gcgtcgcggt cggccggcat
     2461 gtcgaagttg atcacgcggc acacgtcgtc gacgtcgatg ccgcgggccg ccacgtcggt
     2521 cgctacgagc gtgctgacgc cacccgaggc gaacctggcc agcgccttct cgcgctggga
     2581 ctgcgacttg tcgccgtgca tggcgaccgc ctgaacgtcg tggccgcgga ggcgcttgac
     2641 gagccggtcg gcgccgcgct tggtgcggac gaacacgagc gtgcgatcgc cgcccctgag
     2701 ctcacccacg agcgcgtcga tgcgaccgtc gcgggccacc tgcacgaagc ggtgctccac
     2761 gtcggccttg gtcttggccg gcgcggcgtg cacatgccgg cgcgcgtcgc gcgtgtacga
     2821 gcgtgcgatc tggccggcct caccctcgag cgtggcggag aagaacagcg tctgccggtc
     2881 gcgcggcgtc ttggccacga tgcggtcgac cgcgggcttg aagcccatgt cgagcatccg
     2941 gtcggcctcg tcgaggacca gcacctgaac gtgcttcagc gtgacggcgc cgcgctcgag
     3001 caggtcctcg agccggccgg gcgtggccac cagcaggtgg gcgcgcgcgg cggccttggc
     3061 ctgcttctgg atgccgaccc caccgtagac ggccgccacc gacagggcgc gggccgtggc
     3121 caggtcgcgc agctcctcca cgatctggct ggcgagctcg cgcgtgggcg cgaggatgag
     3181 cgccgccggg cggcgggcct ggctctcgat cgagtcgagc agcgggatgc cgaaggcgag
     3241 cgtcttgccc gatccggtgg gggactgagc gagcacgtcg cggccggcga gcacgtcggc
     3301 gatcacgagc ctctgcactg cgaagggctc gtcgatgcct cgcgccgaga gcgcggagac
     3361 gagcggattg gaaacgccga gatcggcgaa ggactgcgaa gacaagtgcg aactccttgt
     3421 gaacaagcgg ggagattcag gtaccccgga agcgctcaca agcgcacaga gagatatgaa
     3481 cccccgggcg ccagagtggc tgcccgaatt agcgaaccta gcagcgctcg ggtgggttcc
     3541 taccatcggg atggtgcgca gcgcgctcgt agctgcggcc ctcgtggtcg ccctcctagc
     3601 cgcctcgccg gccgcggcac agccggtgct gcgcctcgac ggcgaccgcg tggagctccg
     3661 cgacgacccg catcttcccg ctctggacct accctcaccc cccgtttcgg ggatgcgctc
     3721 cgccggagtg cgccgtgcgc aggtcaagcg caccgtccgc ggcgagctgg cgcgcctgct
     3781 cgccgagggg cagatcgacc aggccacgca cgattcccac cggtcgacct acaacctggc
     3841 gcgtaagtca tacaagcagc ttacgggggc ccggaaggcc ggactgggcg gagcgctcga
     3901 gaacgcggag ctcgtggccg ccagcgggca gctcacgccg tcccgattgg cgcccctgtt
     3961 cctcacggtg gcgcgcaacc gccagtggtg gaccaccgga ccgctcctct ccaacgggca
     4021 gcgcgtggaa ttcgctggga gcgaactcgt ctggcagcac tacaccggtc aggggatcca
     4081 gctccaggtg ctggcgaact tcggcaaggc caacgggctc tggcaggcga aggacgacga
     4141 cgccctgcgc gcgctgctcg acgagatgct gccgctcgcg agcgaccgcg acggcatcct
     4201 cgcgtgggag tactggttcc ggttcggcgg cgggtcgccg ccgtgggcga gcgggatggc
     4261 gcaggccacc ggggtacagg cgctcgcgcg cgcggcggtg cagctcggcg agccggcgta
     4321 ccgcgatgcc gcgatccgcg cgctcgcgct gttcgagacg cccgcgccgc gcggcgtgcg
     4381 cgtggacggc gaggccgggc cgcattacct cctctacagc ttcgcccccg gcctgcgggt
     4441 cctgaacgga ttcctccagg ccgtgatcgg gctacacgac gccgccgagc tgctggccga
     4501 gccgcgggcg caggcgctgt tcgaggcggg cgacacccag gcgcggcgcg agacgcagct
     4561 gagcgacacc ggcgcgtggt cgctctactc gaccggcgac gacccggccg ccgggcgcga
     4621 gtcgagcctc cactaccacc gcgtggtcac cgacttcctc gagcgcctct gcgaccgcgt
     4681 cgccgccggc gagtactgcg tggcggccga tcacttcaag gcctacgagg acatcgcgcc
     4741 ggtggtgcgc ccgctcacgc ggcgcgtgcg cgcgggcaag cccgccaacc tgcgcttcgt
     4801 gctctcgaag gtcgcgcgcg tgaggctcca ggtggccgac ggcgaccgcg tcgtggccac
     4861 gcgcacggcc acggtcggct acggcaagcg ctctctcgcc tggaccccgc gctcgcccgg
     4921 cacctacgag ctgcgcgcga ccgcggcgga cctggccggc aacgactcgg tcgagcgcgc
     4981 gatcgagctc gaggtgctgc cgccgcgccc gcccaagcga cgctaggcgc cgcctccgtt
     5041 tatcgtgcgc cgctcccggg gtaccgggag gaatccgggg tcgagagagg agcgcgatga
     5101 cgcagagcgg gagaggtcgt acacgggtgt ggcttgccgc cggaaccgcc ggactgctcg
     5161 ccctcggcgc gaccgcctgc ggcggcagcg agagctcctc gagcagcatg atggaccccg
     5221 tcacgacggc cctccagacg cccggcgtcc gcaccgttca gatcgcgcag cagaagttcg
     5281 acctcgaggt gatcgtcccg ccgtgcccgc gcccgggcga gaaggcgtcg cccaacccgc
     5341 agagcgagca ggacgtgcag accggccggg tcgtcccgcc cgagggctcg aaccggatca
     5401 tcatcccgcg cgagaccctc acccagaccg tcgccgtcca gccgtgccag gtcgattcga
     5461 gcggcgactc gagcagcagc tcgtcgtcct cctcgtcctc gtcctcgtcg tccagcgccg
     5521 ccaccggtca gaccgtcagc accgtactcc tcacgccggg cgggctggcg ccgcaggggg
     5581 cggcgatcca ggagcaggcg ggacaggcgc agaaccagct cgtgctgccg tcgaacgcga
     5641 acatccagac cctcgtgatc ccgccgtgca cccagcccga gcagtcgagc tcgtcgtcct
     5701 cgtcggcggc cgagccaccg cagaagaatc tcgtgctccc cgccgacagc cggaccaagt
     5761 cggccaccgc gccgggctgc accgtgcagc agagcggcta gccgaccgcc agcttcaccc
     5821 gcgggtccac gtggcccgcg gcaccgaggg cgcccacgaa cgtggcgacg agcaggtcgc
     5881 gcagctcgtc gcggctcgcc tcgccacggt cgagccagtc gaggatcgcg ccgtccatcc
     5941 cccatagcca ggcgcggacg gccagccgca gcgccgggcg cggcgcgccc tccgatatcg
     6001 cctcgacgat ccgcaccacg gtccgctcgc gcacgtcctc gataagggag cgcacgtcgg
     6061 gcgaacccgc gctctccagc aggcgccggt agccgctcgc gttctcctcg atccacgcca
     6121 ggtaggcgcc gaggctgccc gcgagctgct cgaccggcgg gcggtccggg tcgggctccg
     6181 tcaccgcggc cacctcggcc ccagccgcgg ccagggtggc ggtgaagaag tcgcgcttgc
     6241 tcggaaagta gtggtagagc aggcccttcg agatccccgc cgccttcgcg acatcgctca
     6301 tcgcgacctc gtcgtaggcg cgctcggtga atgcgcgtgc gcccgcctcg aggagctggc
     6361 ggcggcgctc gtccacgtcg agccgggtga aggcgggcgt ccccacgggc ggaccgtacc
     6421 acctactgcg gctagttgac cgatggtcag tagtcgtgct acgttgtgtt gaccggcggt
     6481 cagcaaggct gccgcccggc aggggatcgg atcgccgcac gcgcggcgaa ttgctttcgg
     6541 agacggagga gcttggatcg atggacgtac tggagctggt tcgcgggcac ggtcggcggc
     6601 tcgacgcggt cgagcgtgag atggcgggga tgagcgagcg cctcgcgcgg ctggagggcg
     6661 tgccggcgga gcggccgcag gcggagcggc cgccgcggcc ggcgccggcg ggcgcggagc
     6721 gaccggcccc gcgcagcacg cggcccgagg gctccgtgcg cgagtggctc gacccctcga
     6781 cctggaacgt cccgcccacg gcgcccccgg ccgccgggca ggccccgccc cccaccggcc
     6841 cgccgcagca gccggcgcgc ccggccgccc gcccctcgca gcgccccggc gggatgcccg
     6901 cgcggcccgc ggccggcccg acccggcccg cccagcccgc cgctccgcgg atcgacctcg
     6961 aggagctcct cggcggccgg atcctcggcc tcgccggcgc gctcgcggtg ctgctcggcg
     7021 tcgccttcct cgtcgcgatg gccgtcgacc gcggctgggt cgacgagccg acccgcatcg
     7081 ccatcggctt cggcggctgc agcgccctcc tgctcggcgc ggtcgccctc tacgagcggc
     7141 gcggtcgcac ccaggccgcc ctcgcggcgg ccggtgccgc cgtggcgggc ctgttcgcca
     7201 cgctcacggc gggcgcgcag ctctacgagc tgtacccggt cccggtgtcg ctcgcgatcg
     7261 ccgcggcgat cggcggcgcc gcaaccgcgc tcgcgctgcg ctggaacgcg cgcacctttg
     7321 cagcgctcgg cctggtcggt gcgctgctcg cgccggtcct cgtcggcgcc gaccaatcgg
     7381 gcctgacgat cgcgttcgtc ctgttcgtgc tcacgatcgc cgtcgcggtc gccgaccgcc
     7441 ggggctggtc cgagtggctt ccgctgaccg cctcgatcgt cgcggctccg cagatcctcg
     7501 actggctagt ggacggcccg gatgccgtcc ccaccgtgct cgtgctgtcg tgcttctggg
     7561 ccgccaacgc ggtcgcggcg gcgagcctcg agcgcacccg cggcgggtcc accatgcacc
     7621 cgatcgccgt gatcctcctc ggcggagcgt ccgcgctcgt cgcgcccacc ggattcgccg
     7681 cgctcgacga gctcgggctc cacgaccaag cggtcgcctg gctcggcgcc ctcggcgcgg
     7741 cgcatgcggg cctcggcctc gcggcgatcg gcacgcgacg cgcctcccgc gatctggcgc
     7801 acctctcgat cgcgatcggc atcggactcg ccgacgtcac gttcgcgctg gcggtcgagg
     7861 ggttcgcgct ggccatcggc tgggccgcct cggccgcggc gctgaccgca ctcgcgcggg
     7921 tccgccacgc ggcggccggc tcgcccgcag ccgacgccgg aaacggaggc gacgcgaagc
     7981 gcgaccccgc cgcgggcgag accgtccgcc ttgccgccgg ggacaccgcc cgcctcaccg
     8041 tcgccgccgc cgtgcagatc gggctcgcgc tagcgcacgc cctcacgctc gacgccgatc
     8101 cgacgcagct ctccggccgg cctgaggaca tgctcgcggg tgtcgccgcg ctcgtcgcct
     8161 gcgggctcgc cgcgctcgtc tcggcacggc tgctcgcccg cgacccgctc gtccgccacg
     8221 gtgggcacgc gatcgcgatc gcgaccctcg cctacctctc ggcgttcgtg atcgacgggc
     8281 agttcctcgc cgtggcgtgg gccgtcgagg cggtcgccgt catggcgctc gcgcggcggg
     8341 ccagaactgg cgttgacccc gtcggctcga tcagctccgg cgccctcgtg ggcctcgccc
     8401 tcacgcacgc gatcacgatc gacgcgcagc cgggcgagct cgccgggccc ccgcacgaac
     8461 tgctcgcggg cgtggcgtca ctcggcgccg tcggcctcgc cgcgctgctc gccgcacagc
     8521 tcttgccgcg caccggcccc ggccgcgcgg ccggtcacgc cgtcacgctg ctggtgctcg
     8581 cctacctgag cgcgttcgtc atcgacgggc cgctgctcgc ggcggcctgg gccgtcgagg
     8641 cggtcgcgat cctcgcgctc gcccgccacc gcgacggcgg gatcgacgcc gtcggctcca
     8701 tcggcgcggt ctcgctgctc gcgttcgcgc tgctccacgg gctcgtgctc gaggcggcgc
     8761 cgcgcgcgct cgtctacggg gtcgaggacc tgctcgacgc cgccatcgcc ctcgccacgc
     8821 tggccgccgc ggcgctcgcg atggcgcggc tgatcccgga ggagcgccgt gacgagcggc
     8881 tcgggttcgt gagcctcgcc gccgccgcgc tcgtctacct gggctcggtc gcgatcgtga
     8941 ccgcgttcca gccgaccacc gagggtttcg acaccgccgt cggcctgctc gacgagcgcc
     9001 agcagggcca gctcctgctg agcgcgttct gggccctggc cggtctcggt gcgctgatcg
     9061 ccggcctcgc gcggcgccag cgcgcgatcc gcgtcggtgg cttcgtactg ctgggtatag
     9121 ccaccgcgaa ggtgttcatg ttcgacctct cgacgctcga ctcgctctac cgggtgggct
     9181 cgttcgtcgc gctcgggctg ctgctgctcg gcgcggcgtt cgcctggcag cggacccgcg
     9241 ccctacgcga cggcgcgggc gctccgaacg gcggggaggc gggagcgtga gcggcccgga
     9301 gtcgcccggc accgcgtggc cgatcgcacc gcagcggccc gcgccgccgg acgcccgccc
     9361 gtcgcggcgg gcgcgcacgc tggcgatcat cggcacggtc gcggcgctgc tcgcgtcgct
     9421 cgtgctcggg ttctacgccc tgctgttcga cgcgttcgcg tgcttcgagt cgtgcgtcga
     9481 gggcagcgga gactggaggg acgatgtgga cgcctggcag tacgacgtcg agctcggcct
     9541 ggcgggggcc ggcgtagtcc tcgccgtggt ctccgtgatc cttgcctcga ctggccggct
     9601 ccgccccgcg ggctatgcgg tcgccgcggc cgccgtgacg ttcgccggtt ggtggacgtt
     9661 cgtgactagc tgagctcggc ggccggctcc cagaccgact cgagcggcac gccgatgatc
     9721 ttcatccggc gggtgagccc gcgcagcgcg aactcgcgca gctcctcggt ggtcaccccg
     9781 agcacgccgg cgtcggtgcc gtcgggcgtc tcgagcgagc ccgcgaccgc gggcagcaga
     9841 tcgcgcagcg tctcccgcac tacggcggcg gcggccggat cgtcgcgcac cgcctcgcgc
     9901 aggtaccagg tcccgtagcc gatgtggcgg tgctcgtcgt ggtggatgag cgtgtagccc
     9961 tccacgaatc ccggcaacag gccctcgcgc tcgaggtagc gggtcgcgaa ctcgaacgtg
    10021 gtgagcccga gggtggcctc gaggatctgg tggtagatcg tgacgaagcg gaccttcgcg
    10081 gcccggtcgg cgggcgccgc gacaagacgc tcgtgcgcgt ggacgagctg ctcgtcgaag
    10141 atgacccgga aagagtcgga cacctgctcg cgtgcgcgct cgacgtgcgc cgctacggtg
    10201 gccggcgcgg ctaccacctc gtcctggaaa cgcgcgtaga actgcatgtg ccgcgcctcg
    10261 tcgacctgct gggtcgccag gaacgtggcc tcctcctcgc tgccgtaggc ggccaccaga
    10321 ccggagaact tcgtggtgat ccgctcctcg gccaccatca gcgacccgag cacgaacagg
    10381 acgagcgcgc ggttcgcctc gcccattccc ggccactgct cgcggtcgcg cgcgagatcg
    10441 atcgcgaacg ggttccactg ggcgtcctcc cagtggcggt agagcgtctg cgggtcgtgg
    10501 aggtgcggct cggtcacgtg cagcagagcg acgcggtctc gcgcgtggcg cagccgcagc
    10561 cggtctcgcc gcagccgcac gcggcggcgt cggcctcggc gcgccgcgac tcacccgcct
    10621 cctccgcgtc ctcgtgcgtg gcgaagacct cccacggcgt gccgtcgggg tcgtgcgccc
    10681 agattttgtc ctggcgggcg tagcagcagg tggtgtccat ctcatcgaac gcggcgaggc
    10741 ccgcggccac gagccgctcg cgcgccgcga gcacgtcgtc ggtcgaggcg acctgcaccc
    10801 cggcgtggtt gaaggcgccg agcgactcgc gctcgccctc gttgagcgtg aagttgagcg
    10861 ccggctcggc cagcgagaac ttcgcgtagc ccgacctcac cttctcggga gctgctccga
    10921 acagcgcctc gtagaacggg atcgaccgct cgacgtcggt gacggtcagc gccaggtggg
    10981 gtctcaggac agccatcgct tcagcacctc cagcgcctcc gggcgcacgt agtagtaggc
    11041 ccacagcccg cgccgctcga cgccgaccag gccggcctcg cgcagcttct tgagatggtg
    11101 ggagagcgtc tgctgggtga cgtcgaacag cgggacgagc tcgcagacgc agacctcccc
    11161 cgcgtgccgc gcgagcacgt cgacgagctg gacccggatc gggtccgaca gcgccttcgc
    11221 cacagcggcg agccgctccg cctcggccgc cgacacgtcg ggccgcgcca ccggcgcgca
    11281 gcacgttgcg cccgcggggc gcttctgctt cggggcgagc gttagatcga cagtcatctg
    11341 tagatgacta tacagatcaa cgtcgatgca acggcccagg
//
LOCUS       DASVPL010000178         7315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_61022, whole genome shotgun sequence.
ACCESSION   DASVPL010000178 DASVPL010000000
VERSION     DASVPL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61022"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VF517_09230"
     CDS             complement(<1..942)
                     /locus_tag="VF517_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8053163.1"
                     /translation="MTRALGAAALGLVLLIGGLAFGTPSLYLPAVALILLGVGAAVWV
                     ALAAAGAAVERSPGPHTVEEDQPWPLVLDSRPGIVPPPGGELTEPLLGRPLRVGFGAP
                     RRVRIDVRFERRGRRLLEPARLSIRDPLGLAERVTESAVDEVLVLPRVESVVAPGSGA
                     SGSIGSRSARPLAESAELEIDGLRPYREGAPAGRIHWPVLARGGEMMERRLVADSDSR
                     PLVVLDARRPPGEEALDRAVRATASVAVHLARGGSCALLLPGDRRPTELDPELRSWAA
                     LHVRLALIEPDERPPAAGRIERTGAVIWVAAAPGVPPG"
     gene            complement(947..1897)
                     /locus_tag="VF517_09235"
     CDS             complement(947..1897)
                     /locus_tag="VF517_09235"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8053164.1"
                     /translation="MSTVPQLDPAGVQALGERLAANVQRAVKVPSSTLRDVIVALVAE
                     GHVLIEDNPGVGKTALARSLARTVDAEYARIQCTVDLLPADVVGTNVFNQRETRFEFR
                     PGPVFANVVLVDEVNRASPKTQSGLLECMQERHVTVDVHTHELARPFVVVATQNPVEY
                     EGTYPLPEAQLDRFMVRVELGYPSPDDEAEMLGDHATGDRVLELEPVASVGEIVAAQE
                     AAGRVHASEALRRYVVAILDATRSDPRLDLGASPRAGLMLLKAAKALAALDGRDHALP
                     DDVQALAPSVLPHRLLLAPDAAGTDRATVVREALERVPAL"
     gene            complement(1955..3550)
                     /locus_tag="VF517_09240"
     CDS             complement(1955..3550)
                     /locus_tag="VF517_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8053165.1"
                     /translation="MSLTFELELKNFRCFPIDDPARIVIGPGFTALVGVNNSGKSSIL
                     RMFWEFRNAFLQFRDLNKVKVASEQRWGTGGPKGAHDTEEVLSNLNDLDMEIGFALRS
                     EGGADGDAATRVTLLIPRSDPSTVQLRWEIRGRPVPELNWAETAAIDPVTGVQADIEP
                     FLQLFRALAESVYLGPFRNAINVGAANYYDLQIGSGFVEQWDQFKSGPSKRQNRAAVE
                     LTAELRRIFGLTTLELNAAPGNQNLQVIADGHSYRLDEQGAGLAQFVVVFAYVAIKRP
                     SFVFIDEPELNLHPSLQLDFLTTLAAYTTHGVVFSTHSLGLARTVGNRVFSVRRVDPG
                     RSVVRPFEETRSRAEFLGELSFSGYQELGYSRVLLVEGPTEVAALQRILRHYDAVHEV
                     VLLPLGGSSLIHAGSVDALDEIRRLSDDVWVLIDSERRAPDAELDPPRASFLENCEKL
                     GFTTHVLDHRAVENYFTDRAVRAALGEQYRALGPHERLADVASPWSKSENWRIAGELS
                     REELDSTDLGRFLSELTEEPTQA"
     gene            complement(3572..4369)
                     /gene="trpC"
                     /locus_tag="VF517_09245"
     CDS             complement(3572..4369)
                     /gene="trpC"
                     /locus_tag="VF517_09245"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX8053166.1"
                     /translation="MSVRLEELIDATRARLERVKHERPLAELEQHASARGEGRPFAEA
                     LSRPGTSLIAEYKRRSPSAGTIREGASVTDIARAYERGGAAALSILTEEKHFGGSLDD
                     LREARETSDLPILRKDFTIDPYQLWEAKAAGADAVLLVVGALRPHELGSLHGLARDLD
                     LDALVEVHDEDELRVALEIDADVIGINNRNLENFHVDLATTFELLHDVPAGKIVVSES
                     GIVERAQIEELEQVGVDAVLVGEALMRAPDPELAARELTRSEEPTQA"
     gene            complement(4366..5397)
                     /gene="trpD"
                     /locus_tag="VF517_09250"
     CDS             complement(4366..5397)
                     /gene="trpD"
                     /locus_tag="VF517_09250"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX8053167.1"
                     /translation="MPNEILTSAIESLASRRDLSADEAAAVLREIMEGRSSEAETAGF
                     LVALRTKGETVDELVGLARTMRELATRVVVDRPAMLCTAGTGGGRPTFNISTTAAFVA
                     AGAGGTVAKHGNRSATGRSGSADVLEALGARIDLDPAAVGECIESVRFGFMFAPAHHA
                     ATRHVVPVRKALGFRTIFNFLGPLTNPAGATRQLMGVSAPDFMDTIAAALAQLGVERA
                     LVVSSEDGLDEISASGPTRAVEVREGRVERRTITPEELGVEPVPTAEIGAGEPHESAA
                     LARRVLDGEQGANRSIVVLNAAAAIYLTDGAETLEEGARAAEAAIDSGAARETLERFV
                     ERSRELAPS"
     gene            complement(5397..5999)
                     /gene="hisIE"
                     /locus_tag="VF517_09255"
     CDS             complement(5397..5999)
                     /gene="hisIE"
                     /locus_tag="VF517_09255"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEX8053168.1"
                     /translation="MQEIAFDDRGLVPCVVQDWRTGEVLTLAYMNEEALTRTRESGQT
                     HFWSRSRQELWHKGATSGNVQQVRALRYDCDLDAVLALVEPAGPACHTGERTCFHNGD
                     TATAAPHEALPALERTIASRRDERPEGSWTAKLLADPPLIGAKVREEAEEVTRAAREE
                     SDERVAEEAADVLYHLTVLLAARGLTLADALGVLNARRSG"
     gene            6036..>7315
                     /locus_tag="VF517_09260"
     CDS             6036..>7315
                     /locus_tag="VF517_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8053169.1"
                     /translation="MDATDLAYAGIARQAELIRARELSPTELVNLYLDRIERIDPRLN
                     AFRVVFADRARAEAQQAEGRVGAGDERPLLGVPVAIKDNIDTAGDLTTNGSAAYGEPP
                     QRDAEMVRRLREAGAIVIGKTHLPELAIWPATESDAWGITRNPWDTSRTPGGSSGGSG
                     AAVAAGLAGAGLATDGGGSIRIPAACCGLVGLKTQRGRVPLGPHVEHWHGLSVAGSVT
                     RRTIDTALWLDVVSGGEGATAPEPDRTFVESARSAPGKLRVAVSTKPSDPRTKVEEPV
                     RQAIHQLAGVLRSLGHDVQERDPDYGWLLPLFFPRWANGISEDVESLARPEAIEPRTR
                     RAAAVGRMLGARSVRWARGREEERTRQINGVFDDCDVLLTPTMPHAPIEAGFFARAGL
                     ARSIDRASGTVTFTQPWNVTGQPAMSIPTPASGP"
BASE COUNT         1022 a         2630 c         2663 g         1000 t
ORIGIN      
        1 gccgggcggc acgccgggcg cggcggccac ccagatcaca gcgcccgtgc gctcgatccg
       61 cccggccgcc ggtgggcgct cgtcgggctc gatcagcgcg aggcggacgt gcagcgccgc
      121 ccacgagcgc agctcggggt cgagctcggt cgggcgacgg tcgcccggga gcaggagggc
      181 gcagcttccg ccgcgcgcca ggtgcacggc cacggaggcc gtggcgcgca ccgcgcggtc
      241 gagcgcctcc tcgccgggcg ggcggcgggc gtcgagcacc acgagcgggc gcgagtcgga
      301 gtccgccacg aggcgccgct ccatcatctc gccgccgcgc gcgagcaccg gccagtggat
      361 ccgcccggcg ggcgcgccct cgcggtacgg gcgcagcccg tcgatctcca gctccgccga
      421 ctccgccagc gggcgggccg agcgcgaccc gatgctgccc gacgcgccgg agcccggcgc
      481 gaccacggac tcgacgcgcg gcagcaccag cacctcgtcc acggcgctct cggtgacgcg
      541 ctcggcgagc ccgagcggat cgcggatgct gaggcgcgcg ggctcgagca ggcggcggcc
      601 gcggcgctcg aagcgcacgt cgatccgcac gcggcgcggg gcgccgaagc cgacgcgcag
      661 tgggcggccg agcagcggct cggtgagctc accgccgggc ggcgggacga tgcccgggcg
      721 cgagtcgagc accagcggcc agggctggtc ctcctcgacg gtgtgcggac cggggctgcg
      781 ctcgacggcg gcgccagcgg cggccagcgc cacccagacg gcggcgccga cgcccagcag
      841 gatcagcgcg accgcgggca ggtacaggct cggcgtgccg aacgcgagcc cgccgatcag
      901 cagcacgagc ccgagggcgg ctgcgcccag ggcgcgcgtc acggcgtcac agcgccggga
      961 cccgctccag cgcctcgcgc accaccgtcg cgcggtcggt cccggcggcg tcgggggcga
     1021 gcagcaggcg gtgcggcagg accgaggggg cgagcgcctg gacgtcgtcg ggcagcgcgt
     1081 ggtcgcggcc gtcgagcgcc gccagggcct tggcggcctt caagagcatc agccccgcgc
     1141 gcgggctcgc gccgaggtcg agccggggat cgctgcgggt ggcgtcgagg atggcgacca
     1201 cgtagcggcg cagtgcctcg ctcgcgtgca cacgcccggc cgcctcctgc gcggccacga
     1261 tctcccccac gctcgccacc ggctcgagct cgagcacgcg gtcgccggtc gcgtggtcgc
     1321 cgagcatctc ggcctcgtcg tcgggcgacg ggtagccgag ctcgacgcgc accatgaagc
     1381 gatcgagctg cgcctcgggc agcgggtagg tgccctcgta ctcgaccggg ttctgcgtgg
     1441 ccacgaccac gaacgggcgc gccagctcgt gcgtgtgcac gtcgacggtg acgtgccgct
     1501 cctgcatgca ctcgagcagc ccggactgcg tcttcgggga cgcgcggttc acctcgtcca
     1561 ccagcacgac gttcgcgaac accggtccgg ggcggaactc gaagcgggtc tcgcgctggt
     1621 tgaagacgtt cgtgccgacc acgtcggcgg gcagcaggtc caccgtgcac tggatccgcg
     1681 cgtactcggc gtccaccgtg cgcgcgagcg agcgcgccag cgcggtcttg cccacgccgg
     1741 ggttgtcctc gataagcaca tgcccctcgg cgacgagcgc gacgatcacg tcgcggaggg
     1801 tcgagctcgg caccttcacc gcccgctgca cgttcgcggc gagccgctcg ccgagggcct
     1861 gcaccccggc ggggtcgagc tgggggaccg tgctcaccgg tccgatggta gggcggcgcg
     1921 gatctccgtc cgccggtccc cgcgggcacc tccgctaggc ctgcgtgggc tcctccgtta
     1981 gctcgctcag gaaccttccg agatcggtcg agtcaagttc ttctcgactc aactcgccag
     2041 cgatgcgcca gttctcagac ttgctccacg gggaggccac gtcggccaag cgctcatgcg
     2101 gacccagcgc cctgtactgc tcgccgagag ccgcacgaac ggcccgatcc gtgaagtaat
     2161 tctccactgc gcgatgatcg aggacatgcg tggtgaaccc aagtttctcg cagttctcta
     2221 ggaaggatgc gcgcggcgga tcaagctcag cgtcgggcgc acgccgctca ctgtctatga
     2281 gtacccaaac gtcgtcggaa agccgacgga tctcatcgag cgcatccact gagcccgcgt
     2341 ggatcaggct gctgccacct agggggagca ggacgacttc atgaacggcg tcgtagtgcc
     2401 ggaggatccg ctgcagtgcc gcaacctcgg tggggccttc gacaaggagt acgcggctat
     2461 aacctagctc ctggtatccc gaaaagctga gttcaccgag gaactccgcc cggctgcggg
     2521 tctcctcgaa tgggcggaca acgctgcgcc ccgggtcgac gcgacgcaca gagaagacgc
     2581 ggttgccgac cgtgcgggca aggccaaggc tgtgcgtgga gaaaacgact ccgtgcgtcg
     2641 tgtatgcggc gagcgtcgtc aggaagtcga gttgaaggga cgggtggagg ttgagctccg
     2701 gctcgtcgat gaagacgaac gacggacgct tgatcgcgac gtacgcgaac accaccacga
     2761 actgagcgag gccggccccc tgctcgtcaa ggcgatagct gtgcccatct gcgatcacct
     2821 gaaggttctg gttcccgggg gcggcgttga gctcgagcgt ggtgagcccg aagatgcggc
     2881 gtagctcagc cgttagctcg acggcggctc tgttctgacg cttactcggc ccggacttga
     2941 actggtccca ctgctcgacg aagcccgagc cgatctgaag gtcgtagtag ttcgcggcgc
     3001 ccacgttgat cgcatttcgg aacggcccga gataaacgga ctcggcgagg gctcgaaaca
     3061 gctgcagaaa gggctcgatg tcggcctgca cgcccgtgac cggatcgatg gccgcagtct
     3121 ccgcccagtt cagctctggc acgggccgcc cacggatctc ccaccgcagc tgaacggttg
     3181 aggggtccga ccggggaata agcaaagtga ccctggtcgc cgcgtcgcca tccgcgccac
     3241 cctcggagcg cagcgcgaag ccgatctcca tgtcgaggtc attcaggtta gagaggacct
     3301 cctcagtgtc gtgagcgcct ttaggaccac ctgtgcccca gcgttgctca gacgcgacct
     3361 tgaccttgtt gagatcgcgg aactgaagaa aggcgttcct gaactcccag aacatccgga
     3421 ggatggagga cttccccgag ttgttcacgc cgacaagagc cgtgaagccc ggaccgataa
     3481 cgatgcgggc ggggtcgtcg atcgggaagc accgaaagtt cttgagttcg agttcgaaag
     3541 tgagtgacag agctggtggt ccccaccgcc gctaggcctg cgtgggctcc tcgctgcggg
     3601 tgagctcgcg ggccgccagc tcggggtcgg gcgcccgcat gagcgcctcg cccaccagta
     3661 cggcgtcgac cccgacctgc tccagctcct cgatctgagc gcgctcgacg atcccggact
     3721 cggacaccac gatcttcccc gcgggcacgt cgtggagcag ctcgaacgtc gtggccaggt
     3781 cgacgtggaa gttctcgagg ttgcggttgt tgatcccgat cacgtcggcg tcgatctcga
     3841 gcgcgacgcg cagctcgtcc tcgtcgtgga cctcgaccag cgcgtcgagg tcgaggtcgc
     3901 gcgcgaggcc gtgcagcgag ccgagctcgt gcgggcgcag cgcgccgacc acgagcagga
     3961 ccgcgtcggc cccggccgcc ttcgcctccc agagctggta cgggtcgatc gtgaagtcct
     4021 tgcggaggat cggcaggtcg ctcgtctcgc gcgcctcgcg caggtcgtcc agtgacccgc
     4081 cgaagtgctt ctcctcggtg aggatggaga gcgcggcggc gccgccgcgc tcgtacgcgc
     4141 gggcgatgtc ggtcacgctc gccccctcgc ggatcgtgcc cgccgagggg gaccggcgct
     4201 tgtactcggc gatcagcgac gtgccggggc gcgacagcgc ctcggcgaac gggcggccct
     4261 ctccgcgggc ggaggcgtgc tgctctagct ccgcgagcgg gcgctcgtgc ttgacgcgct
     4321 ccaggcgggc gcgggtggcg tcgatcagct cctcgaggcg gacgctcacg acggcgccag
     4381 ctcccggctg cgttcgacga agcgctccag cgtctcgcgc gcggcaccgg agtcgatcgc
     4441 ggcctcggcc gcgcgcgccc cctcctcgag cgtctccgcg ccgtcggtca ggtagatcgc
     4501 ggcggccgcg ttcaggacga cgatcgagcg gttggcgccc tgctcgccgt cgagcaccct
     4561 gcgcgcgagc gcggcgctct cgtgcggctc gccggccccg atctcggcgg tgggcaccgg
     4621 ctccaccccg agttcctcgg gcgtgatcgt gcgcctctcg acacgcccct ccctcacctc
     4681 cacggcgcgc gtggggccgg aggcactgat ctcgtccagc ccatcctcgc tcgacactac
     4741 cagcgccctt tcgaccccaa gctgggccag cgcggccgcg atggtgtcca tgaaatccgg
     4801 ggcggagacg cccatcagct gccgcgtcgc gccggccggg ttggtgagcg gtccgaggaa
     4861 gttgaagatc gtgcggaagc cgagcgcctt gcggacggga accacgtgac gcgtcgccgc
     4921 gtggtgcgcc ggagcgaaca tgaagccgaa cctgacgctc tcgatgcact cgcccacggc
     4981 cgccgggtcg aggtcgatgc gggcgcccag ggcctcgagt acgtcggccg agccggagcg
     5041 gccggtcgcg gagcgattgc cgtgcttggc caccgtgccg ccggccccgg ccgccacgaa
     5101 tgccgccgtc gtcgagatgt tgaaggtcgg ccgcccgccg cccgtgccgg cggtgcagag
     5161 catggcgggg cggtcgacca cgaccctggt ggccagctcg cgcatcgtgc gcgccaggcc
     5221 gaccagctcg tcgacggtct cgcccttggt ccgcagggcg accaggaagc cggctgtctc
     5281 ggcctcggac gagcggccct ccatgatctc gcgcaacacc gcggcggcct cgtcggccga
     5341 gaggtcccgg cgcgacgcga gggactcgat ggccgaggtg aggatctcgt tcggcactag
     5401 cccgagcgcc tcgcgttcag cacgccgagc gcgtccgcga gcgtgagccc gcgcgcggcc
     5461 agcagcaccg tgaggtggta gagcacgtcg gcggcctcct cggccacgcg ctcgtcggac
     5521 tcctcgcgcg ccgcgcgcgt gacctcctcg gcctcctcgc gcaccttggc cccgatcagc
     5581 ggcggatcgg cgagcagctt ggccgtccag ctgccctcgg ggcgctcgtc gcggcggctg
     5641 gcgatcgtgc gctcgagcgc cggcagggcc tcgtggggcg ccgcggtcgc cgtgtcgccg
     5701 ttgtggaagc acgtgcgctc gccggtgtgg cacgccggtc cggctggctc cacgagggcc
     5761 agcaccgcgt cgaggtcgca gtcgtagcgc agggcacgca cctgctgcac gttgccagac
     5821 gtcgcgccct tgtgccagag ctcctgacgc gagcggctcc agaagtgcgt ctgcccgctc
     5881 tcgcgcgtgc gcgtgagcgc ctcctcgttc atgtaggcga gggtcaggac ctcacccgtg
     5941 cgccagtcct ggacgacgca cgggacgagt ccgcggtcgt cgaacgcgat ctcctgcatg
     6001 cgggggcgat tctaggtgtg gcaccctccc cctccatgga cgccaccgac ctcgcatacg
     6061 caggcatcgc gcgccaggcg gagctgatcc gcgcgcgcga gctatctccg accgagctgg
     6121 tcaacctcta cctggaccgg atcgagcgga tcgacccgcg gctgaacgcg ttccgcgtgg
     6181 tcttcgccga ccgcgcgcgg gcggaggcgc agcaggcgga gggccgggtg ggcgccggcg
     6241 acgagcgccc gctgctcggc gtgccggtgg cgatcaagga caacatcgac acggccggcg
     6301 acctgaccac caacgggtcg gccgcgtacg gcgagccgcc acagcgcgac gcggagatgg
     6361 tccgccgcct gcgcgaggcg ggcgccatcg tgatcggcaa gacgcacctg cccgagctcg
     6421 cgatctggcc ggccacggag tcggacgcgt ggggcatcac ccgcaacccg tgggacacct
     6481 cgcgcacgcc gggcgggtcg agcggcggct ccggcgcggc ggtggccgcg gggctggccg
     6541 gcgccgggct cgccaccgac ggcggcggct cgatccgcat cccagccgcc tgctgcgggc
     6601 tcgtcgggct gaagacgcag cgcggtcgcg tgccgctcgg gccacacgtc gagcactggc
     6661 acgggctgag cgtggcgggc tcggtcacgc ggcggaccat cgacacggcg ctctggctcg
     6721 atgtcgtctc cggcggcgag ggcgccaccg cgccggagcc ggaccgcacg ttcgtggagt
     6781 ccgcgcgctc ggcgccgggc aagctgcgcg tggctgtgtc gaccaagccg tccgacccgc
     6841 gcacgaaggt cgaggagccg gtgcgccagg ccatccacca gctcgcgggg gtcctgcgct
     6901 cgctcgggca cgatgtgcag gagcgcgacc ccgactacgg ctggctcctg ccgctgttct
     6961 tcccgcgctg ggcgaacggg atatcggagg acgtggagtc gctggcgcgg cccgaggcga
     7021 tcgagccgcg cacgcgccgg gccgcggcgg tcggccggat gctcggcgcg cgctcggtgc
     7081 gctgggcgcg cgggcgcgag gaggagcgga cgcgccagat caacggcgtg ttcgacgact
     7141 gcgacgttct gctcacgccg acgatgcccc acgccccgat cgaggccggc ttcttcgccc
     7201 gcgcgggcct cgcgcggagc atcgaccgcg cctcgggcac cgtgaccttc acccagccgt
     7261 ggaacgtcac cggccagccc gccatgtcga tcccgacgcc cgcgagcggc cccaa
//
LOCUS       DASVPL010000179         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_376058, whole genome shotgun sequence.
ACCESSION   DASVPL010000179 DASVPL010000000
VERSION     DASVPL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376058"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3298
                     /locus_tag="VF517_09265"
     CDS             <1..3298
                     /locus_tag="VF517_09265"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8053170.1"
                     /translation="GDGTRAAELHGQEPGGGWRLHARARVTPLSPDRPAEPVALERLR
                     ERCGAALGGEEFYARVRARGIDFGPSFQTVREIARCDGTALASLAAPPTNGTGRPHPT
                     VVDACFQVLGALVEDESDERAYVPVRIERLELGGNPAAWCRATATRSDTAGALTGDVE
                     LLDDEGGIVGRATGVELRALPREALTASQPSGPAVYEVGWRPAPLEPGTNGAGPGTWL
                     VLGGGETDALARELTVLGAAAIRPAADAAGPSAAVASALSGDEQVRGVVYVADGAPAG
                     DPPTAALEGTATVLELVQTLSEQSAPPRLWIVTHGAQPAGDGLEHPDQSPLWGLRRVA
                     ALEHPKLRATCADVDGDDRSWAALARELVAGGDEPELALRGAERLVPRLVPARDSGPE
                     RTPDETPVALDIQRRGVLDDLVLCPAERRPPGPGEIEIRVHASGLNFRDVLNALGMYP
                     GEPPPLGGECAGRIVAVGEGVEDLAVGDEVAAIAPAAFATYATTDARLAMRIPAETSA
                     AEAAGLPIAFLTAWYGLERLAGMSRGDRVLIHAATGGVGLAAVRLARRAGAEVFATAG
                     SDEKRSLLRSLGIEHVMDSRTLAFADEVRAKTGGEGVDIVLNSLAGDAVTESLGTLRA
                     GGIFVELGKADLLDEERVAELDVAYHAFDLAELCMRDPDLVAEGLALLHAALTGGELT
                     ALPTRALPLTDAAGAFRHVAQAKHVGKVVLVQDEASGALLDPDATYLITGGHGALGRA
                     VAGWMLDNGSRHVVLAGRNAGASDDLPAGVVSMRADVSDERDVRALLDEIAASLPPLR
                     GVVHAAGVLDDGVLAHQTHDRLATALAPKAAGAWNLHRLTGGLDFFVLFSSVAGVLGS
                     PGQGNYAAANAFLDGLAHLRRGMGEPATSIAWGPWEGPGMGAGATAGRGIAPLPVDYA
                     LDTLGRVLRDGPANPVVVDAEWARLGEAPPILSELVTGGGADTSARATLVEELQRLAP
                     AHRREELVQRIAVDAARVLDLDGPASVDVRRPLGELGLDSLTGLELSNMIGRMTGIEF
                     PATLLFDYPTVDAVAGHVLAELLPDDAAVAPAGDEGSERALDEIERLSDEEAEGLIER
                     SLSALVEGDG"
     gene            3295..>3514
                     /locus_tag="VF517_09270"
     CDS             3295..>3514
                     /locus_tag="VF517_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8053171.1"
                     /translation="MSELSERLKRMTPLQRALFALDRAEAKVAELESARNEPIAIVGL
                     ACRFPGGADTPERYWELLAAGRDAVTEVP"
BASE COUNT          419 a         1297 c         1380 g          418 t
ORIGIN      
        1 cggcgacggc acccgggcgg ccgagctcca cggccaggag cccggcggcg gctggcggct
       61 gcacgcgcgc gcgcgggtca cgcccctgtc ccccgatcgc ccggccgagc ctgtggcgct
      121 cgagcgcctg cgcgagcgct gcggcgcggc gctcgggggc gaggagttct acgcgcgcgt
      181 gcgcgcgcgc gggatcgact tcggtcccag cttccagacc gtgcgcgaga tcgcccgctg
      241 cgacgggacg gcgctcgcgt cgctcgccgc cccgcccacc aacggcaccg gacgtccgca
      301 cccgaccgtc gtcgacgcct gcttccaggt cctcggcgcg ctggtcgagg acgagtcgga
      361 cgagcgcgcc tacgtaccgg tgcggatcga gcggctcgag ctcggcggga acccggccgc
      421 ctggtgccgg gcgacggcga cccgcagcga tacggccggc gcgctgaccg gcgacgtcga
      481 gctgctcgac gacgagggcg gcatcgtcgg gcgcgcgacc ggggtggagc tcagggcatt
      541 gccgcgcgag gcgctgaccg cgtcgcagcc gagcggcccg gccgtctacg aggtggggtg
      601 gcgtcccgcg ccgctcgagc cgggcacgaa cggcgccggg cccggcacct ggctcgtgct
      661 cggcggcggc gagaccgacg ccctcgcgcg cgagctgacc gtcctcggag ccgctgcgat
      721 ccgccccgcc gcggacgcgg ccggcccgag cgccgcggtc gcgtcggcgc tgagcggcga
      781 cgagcaggta cgcggggtcg tgtacgtcgc ggacggcgcg cccgcgggcg acccaccgac
      841 ggcggcgctc gagggcacgg cgactgtgct ggagctcgtt cagaccctca gcgagcagag
      901 tgcgcctccg cggctgtgga tcgtgacgca cggcgcgcag ccggcgggcg acgggctgga
      961 gcacccggac cagtcgccac tctggggcct gcgccgggtc gccgcgctgg agcatccgaa
     1021 gctcagagcg acctgcgccg acgtcgacgg cgacgaccgc tcctgggccg cgctggcgcg
     1081 ggagctcgtc gcgggcggcg acgagcccga gctggccctg cgcggcgcgg agcgcctcgt
     1141 cccccggctg gtccccgccc gggacagcgg gcccgaacgc accccggacg agactccggt
     1201 cgccctcgac atccagcgcc gcggagtgct cgacgatctc gtcctgtgcc cggccgagcg
     1261 gcgcccgccg ggccccggtg agatcgagat acgcgtgcac gcgtcggggc tcaacttccg
     1321 cgacgtcctg aacgcgctcg gcatgtaccc cggcgagccg ccgccgctgg gcggcgagtg
     1381 cgcgggacgg atcgtggcgg tcggcgaggg ggtcgaggac ctcgcggtgg gcgacgaggt
     1441 cgccgcgatc gcgcccgcgg cgttcgccac ctacgcgacc accgacgcgc ggctcgccat
     1501 gcgcatcccg gccgaaacga gcgccgccga ggcggccggg ctcccgatcg cgttcctcac
     1561 ggcgtggtac gggctcgagc ggctggccgg gatgtcgcgc ggcgaccgcg tgctgatcca
     1621 cgccgcgacc ggcggcgtcg gcctggcggc ggtccggctc gcccggcgcg ccggcgccga
     1681 ggtgttcgcc accgcgggga gtgacgagaa gcgcagcctg ctgaggtcgc tcggcatcga
     1741 gcacgtgatg gactcgcgca cgctcgcgtt cgcggacgag gtgcgcgcta agaccggcgg
     1801 cgagggcgtc gacatcgtgc tcaactcgct cgccggcgac gccgtgacgg agagcctcgg
     1861 gacgctgcgc gcgggcggga tcttcgtgga gctcgggaag gccgacctcc tcgacgagga
     1921 acgggtcgcc gagctcgacg tcgcctacca cgcgttcgat ctggccgagc tgtgcatgcg
     1981 cgacccggat ctcgtcgccg aggggctggc cctgttgcac gcggcgctga ccggcggcga
     2041 gctgacagcg ctgccgacgc gcgcgctccc gctgaccgac gccgcgggcg ccttccggca
     2101 cgtcgcgcag gcgaagcacg tgggcaaggt cgtgctggtc caggacgagg cgagcggcgc
     2161 gttgctcgac cccgacgcga cgtacctgat cacgggcggg cacggtgcgc tcggacgggc
     2221 ggtcgcgggc tggatgctcg acaacggcag ccgccacgtc gtgctggcgg gtcgcaacgc
     2281 gggggcgagc gacgacctcc ccgccggcgt cgtgagcatg cgagccgacg tctccgacga
     2341 gcgcgacgtg cgtgccctgc tggacgagat cgcggcctcg ctgccgccac tgcgcggggt
     2401 cgtgcacgcg gccggagtgc tcgacgacgg cgtgctcgcg caccagacgc atgaccggct
     2461 cgcgaccgcg ctggcgccca aggccgccgg cgcgtggaac ctgcaccggc tcaccggcgg
     2521 gctcgacttc ttcgtgctct tctcctcggt cgccggcgtg ctcggatcgc ccggtcaggg
     2581 caactacgcg gcggcgaacg cgttcctcga cggcctcgcc cacctgcggc ggggcatggg
     2641 cgagccggcc acgtcgatcg cgtggggacc gtgggagggg cccgggatgg gggcgggggc
     2701 cacggccggc cggggcatcg ccccgctgcc cgtcgactac gcgctcgaca cgctcggccg
     2761 ggtgctgcgg gacggccccg caaatcccgt cgtggtcgat gccgagtggg cccggctggg
     2821 cgaggcaccg ccgatcctct ccgagctggt gaccggtggg ggcgccgaca cgtccgcgcg
     2881 cgcgacgctc gtggaggagc tccagcggct ggcgcccgcg catcgccgcg aggagctggt
     2941 gcagaggatc gcggtcgacg ccgcgcgcgt gctcgacctc gacgggcccg cgagcgtcga
     3001 tgtgcgccgc ccgctcgggg agctcggcct cgactccctc accgggctcg agctgagcaa
     3061 catgatcggg cggatgacgg ggatcgagtt cccggccacg ctgctgttcg actacccgac
     3121 ggtcgacgcg gtcgcggggc acgtgctggc ggagctgctc ccggacgacg ccgctgtggc
     3181 gcccgccggg gatgagggga gcgagcgcgc actggacgag atcgagcgac tctcggatga
     3241 ggaggccgag ggtctgatcg agcggtcgct gtcggcgctc gtcgaggggg acgggtgagc
     3301 gagctctccg agcggctcaa gcggatgacg ccactccagc gcgcgctgtt cgcgctcgat
     3361 cgcgccgagg ccaaggtcgc cgagctggag tccgcgcgca acgagccgat cgccatcgtc
     3421 ggcctcgcct gccgctttcc cggcggcgcc gacacccccg agcgctactg ggagctcctg
     3481 gccgccgggc gcgacgccgt caccgaggtc ccgc
//
LOCUS       DASVPL010000180         4128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_334070, whole genome shotgun sequence.
ACCESSION   DASVPL010000180 DASVPL010000000
VERSION     DASVPL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334070"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VF517_09275"
     CDS             <1..450
                     /locus_tag="VF517_09275"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8053172.1"
                     /translation="YQEAFDRARPVLEAMGALVLRVGPQGHGQMAKLINNTLAAVNAE
                     AIAEALVLAQRAGLDAGALLEVVGAGSGNSTMLELKARPMLDRQLDPLFKLEHMLKDV
                     RHCLAEARALGVELPVAERVERLYAAAADEGRGERDFAAVFELLSRR"
     gene            581..1210
                     /locus_tag="VF517_09280"
     CDS             581..1210
                     /locus_tag="VF517_09280"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEX8053173.1"
                     /translation="MPVAFKEWAVTVRALAEGEQLVTLRKGGIREENRHFEIEHDRFF
                     LYPTFDHQQGQHVRESHRPELGRALEEGVWPDGEPSAAVFTNDGGIPQPDRVRIRAWA
                     EVAASYLITDPRCVEALRPYYVWTPDYAEKRLAWKRRHPLHVMLLRTYRIPRPVTVKV
                     RDEYLGCRSWLELQRDLPFEGTPVLSDEEFDRAAEQIEAIASDAVPVLA"
     gene            1332..2231
                     /locus_tag="VF517_09285"
     CDS             1332..2231
                     /locus_tag="VF517_09285"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX8053174.1"
                     /translation="MHTPPRDAAIAETAGAQHGVLSLPQLQSLGLSASGVRKRAAAGK
                     LHRMYRGVYAVGHSRVTWKGRYMAAVLTCGDGAVLSHRSAARLIGLRRTAGRLDVTVP
                     DRVVRVPGIDAHRTRRLDPRDVIAEDRIPCTSPARTICDLAAHEGDARRTEKDIARAE
                     ELRIFDLGAVQRILRERPGARVVHAALGVETIRSRNELEERFYAICEQAGLPRPLVNM
                     PLVLGDGAHVEADFAWPDLWLIVETDGWATHGTHSAFVNDRRRDRRLALVGWRVLRFT
                     WYEIEHEPERVAAELGALLALAS"
     gene            2242..>4128
                     /locus_tag="VF517_09290"
     CDS             2242..>4128
                     /locus_tag="VF517_09290"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEX8053175.1"
                     /translation="MGGREAKPRGWRARGTRRSILPLLLLAVLATPGAANGATDDTYA
                     RVEPTQVVVGNSLVERRWSRDQLRTTALIDKRDGGLTWSRNTREFALRTGPAEIGSEQ
                     FKVDSAAITQMPRGGLRIRMAITGPALTGTRTIEVYPGIAGFRTQTTLTSPAPLTLNE
                     VVLEEAAVGASTKPTIHALRSGADWRDSSWAGPTPSEDYPGPPLALGDPHAGDWRDTH
                     TATAGTPLQGAAQWFDGDRGDRRLFMQMERNDFPSSWVEYDGATARLRLQYARDVVSL
                     GPFEEQFHAENPNPSGGRTRTMLPGQPFAFEATFTGVGSGVGDAEWQWSKYLLHQRLD
                     PRYPHAITFNSDKNDRNRRSTGAKDDTDIAAVRELAPIARRLGVETFALDDGWQARSG
                     DWCPDSPQCPEPRAATDPTFGPRFPDPTFTAVREAIAPMRLGLWMSPLNFNPSSSTYM
                     QHPDWVCSPLGHALALYNLAQPYEGSNEAGVGEWGNAAYAHVESRIREAITKWGVRFF
                     KFDFMAWLDCTGQNDLYEHHDAFVAMLDRIRRDHPDVTLQIDETNDYRLFPFESVLRG
                     PSWFQNGTPETKQLVHNLWLLNPYVPGFSIGQHVLGGDSYKKESVDTIMAAALPSYIT
                     FWTDL"
BASE COUNT          649 a         1491 c         1421 g          567 t
ORIGIN      
        1 taccaggaag ccttcgaccg ggcgcggccg gtgctcgagg cgatgggcgc cctggtgctg
       61 cgcgtcgggc cccaggggca cgggcagatg gccaagctga tcaacaacac gctcgcggcc
      121 gtgaacgcgg aggcgatcgc cgaggcgctc gtgctcgcgc agcgcgcggg gctcgacgcc
      181 ggcgccctgc tcgaagtggt cggcgcgggg tcgggcaact cgacgatgct cgagctcaag
      241 gcgcgcccga tgctggaccg ccagctggat ccgctcttca agctcgagca catgctgaag
      301 gacgtgcggc actgcctcgc ggaggcccgt gcgctgggcg tggagctgcc ggtggcggag
      361 cgggtcgagc ggctctacgc cgccgccgcc gacgagggcc gcggcgagcg cgacttcgcc
      421 gccgtgttcg agctgctgtc gcgccgctaa ccccgcaaat tgcgggtacc caagcgccag
      481 accgcacaat gccttgaaaa acgggcgccc ttcagtaaga atcccgcgcc gccagaccgc
      541 aagaccgagg ggctagcgga tggctgaatc tctctcattg atgccggtcg ctttcaagga
      601 atgggcagtt acggtgcgcg ctctcgccga gggcgagcag ctcgtgaccc tgcgcaaggg
      661 cggaatccgc gaggagaacc gccatttcga gatcgagcac gaccgcttct tcctctatcc
      721 cacgttcgat caccagcagg gccagcacgt gcgcgagtcg caccggccgg agctggggcg
      781 ggcgctggag gagggcgtct ggccggacgg ggagccgtcg gccgcggtgt tcacgaacga
      841 cggcgggatc ccgcagccgg accgggtccg gatccgcgcc tgggccgagg ttgccgcgag
      901 ctacctgatc accgacccgc gctgcgtcga ggcgctccgc ccatactacg tgtggacgcc
      961 ggactacgcg gagaagcggc tcgcgtggaa gcgccgccac ccgctccacg tgatgctgct
     1021 gcgcacctac cgcatcccgc gtcccgtcac ggtcaaggtc cgcgacgagt acctgggctg
     1081 ccgctcctgg ctggagctcc agcgcgatct ccccttcgag ggcacgcccg tgctctcgga
     1141 cgaggagttc gaccgcgccg ccgagcagat cgaggcgatc gcctcggatg ccgtgccggt
     1201 gctggcgtaa ccctcccgta acgcgcgcgg caccccgttg tcacacgacg gtcttgaacc
     1261 gtcacgcgaa ccgcggcatc aatatgggca tggactgcgc gagtgcagaa acgggacaca
     1321 tggtcccgca agtgcacaca cccccgcggg acgcggcgat cgcggagacg gccggcgccc
     1381 agcacggggt gctctccctt ccccagctgc agtcgctcgg tctgagcgcg agcggcgtgc
     1441 ggaagcgagc ggcggccggg aagctgcacc gcatgtaccg cggcgtgtac gccgtcggcc
     1501 actcccgcgt cacgtggaag gggcgctaca tggccgcggt cttgacgtgt ggggacggcg
     1561 ccgtgctctc gcaccggtcg gctgcgcggc tcatcggtct gcgccgcacc gccggccggc
     1621 tcgacgtgac ggtgccggat cgcgtggtgc gcgtcccggg gatcgatgcg caccggaccc
     1681 gccgcctcga cccccgcgac gtcatcgccg aagaccgcat cccatgcacc tcccccgcac
     1741 gcacgatctg cgacctcgcc gcccacgagg gcgatgctcg gcgcaccgag aaggacatcg
     1801 ctcgcgcgga ggagctgcgg atcttcgacc tcggggcggt ccagcgcatc ctgagagagc
     1861 ggccgggcgc gcgggtcgtc cacgcggcgc tgggggtcga gacgatccgc agccgcaacg
     1921 agctcgagga gcggttctac gcgatctgcg agcaggccgg cctgccgcga ccgctcgtga
     1981 acatgccgct cgtgctcggc gacggtgctc acgtcgaggc cgacttcgcc tggccggacc
     2041 tgtggctgat cgtcgagacc gacggctggg ccacgcacgg aacgcacagc gccttcgtga
     2101 acgaccgccg ccgcgatcgc aggctggcgc tggtgggatg gcgcgtgctc cggttcacgt
     2161 ggtacgagat cgaacacgag ccggagcgcg ttgccgccga actcggcgcg ctgctggcgc
     2221 ttgcgagcta gcctcccgca cgtgggggga cgggaggcga agccgcgggg gtggcgcgcg
     2281 cgaggaacgc ggcgctcgat cctgcctctc ttactgcttg ccgttcttgc aacgcctggg
     2341 gcagcgaacg gcgccaccga cgacacctac gcacgcgtcg agccgacgca ggtcgtcgtc
     2401 ggcaactcgc tcgtggagcg gcgctggtcc cgcgatcagc tgcgcaccac cgcgctgatc
     2461 gacaagcgcg acggcggcct cacgtggagc cgcaacacgc gggagttcgc cctgcgcacc
     2521 ggccccgccg agatcggcag cgagcagttc aaggtcgact cggccgcgat cactcaaatg
     2581 ccacgcggag gcctgcgtat acggatggca attacagggc cagcgctcac ggggacgcga
     2641 acgatcgagg tctacccggg catcgcgggc ttccgaacgc agaccacgct gacatcaccg
     2701 gcgccgctca ccctcaacga ggtagttctg gaggaagcgg cggtcggagc ctcgaccaag
     2761 ccgaccatcc acgcgctgag atcgggcgcc gactggcgcg actcgagctg ggccggcccc
     2821 accccgagcg aggattaccc cggccccccg ctcgcgctcg gcgacccgca cgccggcgac
     2881 tggcgcgaca cgcacaccgc gacggcgggc acgccgctcc agggcgccgc gcagtggttc
     2941 gacggcgatc gcggcgaccg gcgcctcttc atgcagatgg agcgcaacga cttcccctcc
     3001 tcgtgggtcg agtacgacgg cgccaccgcc cgcctgcggc tccagtacgc gcgcgacgtg
     3061 gtctctctgg ggccgttcga ggagcagttc cacgcggaga acccgaaccc gtcgggcggc
     3121 cgcacgcgca cgatgctccc cggccagccg ttcgcgttcg aggcgacgtt caccggcgtc
     3181 gggtccggtg tcggcgacgc ggagtggcag tggtcgaagt acctgctcca ccagcggctc
     3241 gacccgcgct acccgcacgc gatcacgttc aactccgaca agaacgaccg caaccgccgc
     3301 tcgaccggag ccaaggacga caccgacatc gccgccgtgc gcgagctcgc gccgatcgcc
     3361 aggcgcctcg gcgtggagac cttcgcgctc gacgacggct ggcaggcgcg ctccggcgac
     3421 tggtgccccg actcgcctca gtgcccggag ccgcgcgcgg ccaccgaccc caccttcggc
     3481 ccgcgcttcc cggacccgac ctttaccgcg gtacgcgaag ccattgcgcc catgcggctc
     3541 ggtctctgga tgagcccgct caacttcaac ccgtcgtcga gcacgtacat gcagcacccc
     3601 gactgggtct gctcgccgct cgggcacgcg ctcgcgctct acaacctcgc gcagccgtac
     3661 gagggatcga acgaggccgg cgtcggcgag tggggcaacg ccgcctacgc gcacgtggag
     3721 tcgaggatcc gcgaggcgat cacgaagtgg ggcgtgcgct tcttcaagtt cgacttcatg
     3781 gcctggctcg actgcacggg ccagaacgac ctgtacgagc accacgacgc gttcgtcgcg
     3841 atgctcgacc gcatccgtcg cgatcacccc gacgtgaccc tccagatcga cgagaccaac
     3901 gactaccgcc tgttcccgtt cgaatcggtc ctgcgcgggc cgagctggtt ccagaacggg
     3961 acgccggaga ccaagcagct cgtgcacaac ctgtggctgc tgaacccgta cgtgccgggc
     4021 ttctcgatcg gtcagcacgt gctcggcggc gactcctaca agaaggagag cgtcgacacg
     4081 atcatggcgg ccgcgctgcc gtcctacatc acgttctgga ccgacctg
//
LOCUS       DASVPL010000181         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_137848, whole genome shotgun sequence.
ACCESSION   DASVPL010000181 DASVPL010000000
VERSION     DASVPL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137848"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VF517_09295"
     CDS             <1..605
                     /locus_tag="VF517_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053176.1"
                     /translation="ARTPAKHTTMRLLLRYTKPGQPDAKPSPIRRLVIDVPAGTVFHR
                     NALPACGADDAEVMASGPGACPAGSRIGGGPITVVTGFGKPFDPFVSPTEVFNDGRGW
                     LEVSQTPSEPRTTIAVTRLAVTGRRISGDIAATPGGPPDGQSAVSTADLAFGARRYVT
                     TPRTCPRPRRWVTKAWFTYADGRTEAVRTTTPCTPGKRKR"
     gene            804..1451
                     /locus_tag="VF517_09300"
     CDS             804..1451
                     /locus_tag="VF517_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8053177.1"
                     /translation="MTQTRIHNISISLDGFAAGEGQSLDAPMGHAGRRLPAWMFATRF
                     GAPILGRTGGSAGVDNAFAQQHEPGIGAEIMGAGKFGPPGWQDDADWKGWWGPNPPFH
                     TPTFVLTHRPRPSIEMEGGTTFHFLDASPAEALDAARDAAGGRDVRIGGGPTVARDFI
                     AAGLVDHIHLVQVPIVLGRGVRVWDGLEALEERYEVEAVSAPSGVTHLTFTRKTS"
     gene            complement(1483..1555)
                     /locus_tag="VF517_09305"
     tRNA            complement(1483..1555)
                     /locus_tag="VF517_09305"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1520..1522),aa:Gly,seq:ccc)
     gene            complement(1684..1821)
                     /locus_tag="VF517_09310"
     CDS             complement(1684..1821)
                     /locus_tag="VF517_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053178.1"
                     /translation="MPRSEASESRPGAPSFGSSKPGAAGRLPAATALSIESSGSFWAT
                     P"
     gene            1948..2166
                     /locus_tag="VF517_09315"
     CDS             1948..2166
                     /locus_tag="VF517_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8053179.1"
                     /translation="MDSDARAFARRYGLTYPSVRDGDGATRKSFGVNGQPETIVIDRR
                     GRIATTFRGPVTDRDLRRALPPLLEEPA"
     gene            2163..>2464
                     /locus_tag="VF517_09320"
     CDS             2163..>2464
                     /locus_tag="VF517_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEX8053180.1"
                     /translation="MRRTAATALVALLALAGPAAAAACPRTTMGEVEHEVMCLECGVP
                     LDVAENSLQAKQERTYIARQIAACRTKDQVKDALAAQYGDRVLATPKAKGFGLAAYL"
BASE COUNT          333 a          949 c          886 g          296 t
ORIGIN      
        1 ccgcgcgcac gccggcgaag cacacgacga tgcgcctgct cctccgctac acgaagccgg
       61 gacagccgga cgcgaagccg tcgccgatcc ggaggctggt gatcgacgtg cctgccggaa
      121 cggtgttcca ccgcaacgcg ctcccggcct gcggggccga cgacgccgag gtgatggcga
      181 gcggccccgg cgcctgcccc gcggggagcc gcatcggcgg cgggccgatc accgtcgtca
      241 ccgggttcgg caagccgttc gacccgttcg tctcgccgac cgaggtcttc aacgacggca
      301 ggggctggct cgaggtctcg cagacgccgt cggagccgag gaccacgatc gccgtcaccc
      361 gcctggccgt caccggacgg cggatcagcg gcgacatcgc cgcgaccccg ggcgggccgc
      421 cggacgggca gagcgccgtg tcgaccgccg acctcgcctt tggcgcgcgc cgctacgtca
      481 cgacgccgcg cacctgcccg cgcccgcgca ggtgggtcac gaaggcttgg ttcacgtacg
      541 cggacgggcg cacggaggcg gtccggacca cgacgccgtg cacgccgggc aagcgcaagc
      601 gctgaggccg cggctcgcag cggccagtcg ccgcgatgca caccgcgctc gcgcgcgcgg
      661 gcgccgctcg gcggggttgc cttcgcgtgc ggccgctcgg acgaagccgc taggttccgc
      721 cgagccgcgc gggaccggtg gatgccggtg ccggcggctg cctccccctg cgcagagcgg
      781 cccagcaaaa cggaagcgag cagatgacac agacccgtat ccacaacatc tccatctcgc
      841 tggacggctt cgccgccggc gagggccagt cactcgacgc ccccatgggc cacgccggcc
      901 ggcggctgcc cgcgtggatg ttcgcgaccc gcttcggcgc gcccatcctg ggacgcacgg
      961 gcggctccgc gggcgtcgac aacgcgttcg cccagcagca cgagccgggc atcggcgccg
     1021 agatcatggg ggcgggcaag ttcggcccgc cgggctggca ggacgacgcc gactggaagg
     1081 ggtggtgggg cccgaacccg ccgttccaca cgccgacctt cgtgctgacc caccggccgc
     1141 ggccctcgat cgagatggag ggcggcacca ccttccactt cctcgacgcc tcaccggccg
     1201 aggcgctcga cgccgcccgc gacgcggccg gcgggcggga cgtgcgcatc ggaggcggcc
     1261 cgaccgtcgc acgcgacttc atcgccgcgg gtctcgtcga ccacatccac ctggtccagg
     1321 tcccgatcgt gctcggccgc ggcgtccggg tgtgggacgg actcgaggcg ctggaggagc
     1381 gctacgaggt cgaggccgtc tcggcgccca gcggggtgac ccacctgacg ttcacccgga
     1441 agacctcgtg acgaccgcgg agcccgagcg ctcgacgggg taagcggacg acgggtctcg
     1501 aacccgcgac cttcggcttg ggaagccgac gctctaccaa ctgagctacg tccgcgtgcg
     1561 gccctggcgg gccggccgcc agtttagtcc cgctccgtac ccttcgaaag aggtgaagcg
     1621 actcctggca ccggctccgc tcgcggccgt cctgatcgtc gcggcgctcg tggcgctgct
     1681 cgcctacggc gtcgcccaga acgagcccga cgactcgatc gacagcgccg tcgcggccgg
     1741 caagcgcccc gcggcgcccg gcttcgagct gccgaagctg ggcgcgcccg gccggctctc
     1801 gctcgcctcg ctccggggca aggtcgtggt cctgaactac tgggcgtcgt ggtgcccgcc
     1861 gtgccgcggc gagtcgcccg tcctcgagcg ctggcaccgg cggatcgcgc cgaggaacgc
     1921 gacggtcctc ggcatcgacg tcccgacgtg gactccgacg cccgcgcgtt cgcccgccgc
     1981 tacgggctca cctacccctc ggttcgagac ggcgacggcg ccacgcggaa gtcgttcggc
     2041 gtcaacgggc agcccgagac gatcgtcatc gaccgccgcg ggcggatcgc caccaccttc
     2101 cgcggcccgg tgaccgaccg cgacctgcgg cgcgcgctgc cgccgctcct cgaggagccc
     2161 gcgtgaggcg cacggcggcg accgcgctcg tggcgctgct ggcgctggcc ggccccgccg
     2221 cggcggccgc gtgcccgcgc acgaccatgg gcgaggtcga gcacgaggtc atgtgcctgg
     2281 agtgcggcgt cccgctcgac gtcgccgaga actcgctgca ggccaagcag gagcgcacct
     2341 acatcgcgcg tcagatcgcg gcctgccgca ccaaggacca ggtcaaggat gccctcgccg
     2401 cgcagtacgg cgaccgcgtg ctcgccacgc cgaaggcgaa gggcttcggc ctggccgcct
     2461 acct
//
LOCUS       DASVPL010000182        11868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_61420, whole genome shotgun sequence.
ACCESSION   DASVPL010000182 DASVPL010000000
VERSION     DASVPL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11868
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61420"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /gene="leuC"
                     /locus_tag="VF517_09325"
     CDS             complement(<1..1221)
                     /gene="leuC"
                     /locus_tag="VF517_09325"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX8053181.1"
                     /translation="MAKTMFDKIWEAHEVAPGLIYIDLHMVHEVTSPQAFESLRLAGR
                     KVRRPDRTLATADHNVPTDGSTTAAQIRDQLSRVQVETLERNCREFGVPHYSLGSAHQ
                     GIVHVIGPELGITQPGMTVVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTMVQPKP
                     RSMRILYTGTLGFGVTAKDLILGTIGQMGVSGGVGHAIEFAGEAVETLSMEGRMTICN
                     MTIEGGGRAGMIAPDDTTFEWIEGRPAAPDQLPVDEWRALRTDDGASFDKQIEVDATA
                     LEPQVTWGTTPEMVAPVTAAVPEPSTEGEERALRYMDLAPGTAIQDIRLDRIFIGSCT
                     NSRIGDLRAAAEVVKGRKVSADLHAMVVPGSQQVKAQAEQEGLDEVFRAAGFDWRSAG
                     CSMCLGMNPDIAAPG"
     gene            complement(1227..1838)
                     /locus_tag="VF517_09330"
     CDS             complement(1227..1838)
                     /locus_tag="VF517_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8053182.1"
                     /translation="MATATAPLTDAVPSAAVEIGPRIRALREAMGLSLRDLGERAGVS
                     APMIGQVERGETSPTLAVANRIASGLELSLSQLLRLDESEGVTVIRRHQRRPGASRAS
                     GHAYELLTPQLPGQRAEVSRHVLKPGAATGGPDDPPIHEPGSRETAVLIAGRLTLICT
                     GTRYELNEGDAVTFDADLPHRFENPGPDEARFMAVVTAGLRRS"
     gene            complement(1941..2948)
                     /locus_tag="VF517_09335"
     CDS             complement(1941..2948)
                     /locus_tag="VF517_09335"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX8053183.1"
                     /translation="MTFRWLDGERTIVFGPADVGSLEPGFALVTTERAERQLPSLGER
                     ASAVVHVPAGPVPEAAATVREALPPGLPLVALGGGRVIDAAKGIAGADSLSVTAVPTT
                     LSGAPMTRIHRMPAGVDAWNLVRPSLVIADPDLMSSQDPQPRAASAMNALAHAAEALY
                     GPMANPVAEMAALRAAEHLSGGLDLSSEDGNGGNEDKVRLELALGSLLAGYAVGSAGF
                     ALHHLLGQTLVRVTGVSHSAAYAVLLPYTLEAMLERATPAISKLGEALGLGTRNPAAV
                     PARVAELAALAGPRRLGEIGVSEDQIDAVVEEALQRADLANTPDPPGADELRALLHEA
                     L"
     gene            complement(2945..4525)
                     /locus_tag="VF517_09340"
     CDS             complement(2945..4525)
                     /locus_tag="VF517_09340"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX8053184.1"
                     /translation="MESDVGRHERRDEDRVRIFDTTLRDGEQSPGISLNKQEKVEIAH
                     QLARLGVDVIEAGFPITSPGDFEAVQAIAREVHGPIICGLARTSFQDIDAAWSAIQDS
                     ERPRIHTFIATSDIHIERKLQTTREDVKGQARAAVAHAKQYTDDVEFSPEDGSRSDVE
                     FMAEVIQIAIDEGATTINVPDTVGYTMPHEYAAMFEELYRLVPDLRDVVVSVHCHNDL
                     GLAVANSFAGLTAGCRQVECAINGIGERAGNASLEEIVMLLHTRESSVGLWTGAETRE
                     IARTSRMISRLTGYAVQPNKAIVGRNAFAHEAGIHQDGVLKERTTYEIMDAETIGLEA
                     NSIVLGKHSGRHALRKALEELGFQVEGAALNTAFRRFKDIADKKKKVTALDLEAIVSD
                     EMRQSPVAFGLQSFEVEASSQRAPLATVIVRLPNGDLAEGSFTGDGPVDAFFSALNAA
                     TGHEARLREYHVSAVTGGRDALGEVSVLLELGGRLASGAGVSTDILEASGQAYLRALS
                     NALAADIPSEAPSEAVTP"
     gene            complement(4685..5098)
                     /locus_tag="VF517_09345"
     CDS             complement(4685..5098)
                     /locus_tag="VF517_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053185.1"
                     /translation="MWSASTAGGSSRRTAIRAGLPKGVEVVDPRSGTRRLVDRGAGAV
                     RLAPGLVLTYDGRSAGSPGGSEGLRAFDRQGRPRFRMLAGEVVHDVQVLGYHAYARAG
                     DTVHVIDLRAHAVVGRSRVPSEREMHFVVQGGAGD"
     gene            5494..5649
                     /locus_tag="VF517_09350"
     CDS             5494..5649
                     /locus_tag="VF517_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053186.1"
                     /translation="MVATPTCTSVVTRIASRSTIRIPPRPPLGTPLTGVPVEIVNAAC
                     PPSEESA"
     gene            complement(5894..7507)
                     /gene="serA"
                     /locus_tag="VF517_09355"
     CDS             complement(5894..7507)
                     /gene="serA"
                     /locus_tag="VF517_09355"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX8053187.1"
                     /translation="MNAAPQVLVKEKIGESGVQLLRDAGFDVELGVDWDDGELEQRIG
                     DYDGILIRSATKLTAELLAKADRLRAVGRAGVGVDNVDVPAATKRGIVVANAPQSNVI
                     TAAEHTLALLLALARNLPQAHASLTSGKWERSKFSGVELYEKTLGVIGFGRIGQLVAE
                     RAQGFGMRVVAFDLYVSEERFRDMGVQRAETSDELYAAADFVTIHLPKTPETEGWLNA
                     EAFAKMKDGVRVLNVARGPLLVDADLKAALDSGKVAGAALDVFQEEPITDHPLFAYPN
                     VIVTPHLGASTAEATDRAGYQAAEQVVAALTGGVVTSAVNVPAVSAADLEELGPFLPL
                     CANLGRIAAGLAENGSVDAVEAEYLGGIAGRDTRLLTVQVLKGILQGKTEEPVNDVNA
                     STLASERGIVVSETKQPSARDFTDLVRVTVVTGGNRSRVVGTTLGNRHRGHLLEAWGS
                     RFNVQLEDHLAVFRYRDQPGMIGRVGSALGAADVNVDSAAVGHGLDDEPSTGDEAIMV
                     VTTDRPVPDDVMREIVAGDGFHDGRAVSL"
     gene            complement(7504..8682)
                     /locus_tag="VF517_09360"
     CDS             complement(7504..8682)
                     /locus_tag="VF517_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX8053188.1"
                     /translation="MANPYIKSYVMTAGPTPLPPAVSNAMAEPILYHRAPAFIEVYAR
                     CLEKLKGVFQTENDVLTFAASGSGAMESAVANLVEPGEPAFVASCGKFGERWAELADD
                     FGARTIHWDAGWGNKVEPAELERRLAENEGVKVIFTTLSETSTGVVNDVQALTEVAHA
                     AGALIVVDAVSGLGAVPIPQDEWGVDVVVSGSQKALMCPPGLAFASPNAAALERAASI
                     PGRRHYFDWEKTAKGQREDPPDSPFTPAVTLFIALDVALAMMEEEGLEQVFERHRLLG
                     RATRSAVKALDLEIYGPEDENSNVATAIKLPETIDGGKVPKLMRDRYGITIAGGQGKL
                     KGKIARIAHCGYFGAFDILTTLSGFEMTLRELGHDLELGAGVGAAQGVFLDAGVPVAA
                     "
     gene            complement(8921..9655)
                     /locus_tag="VF517_09365"
     CDS             complement(8921..9655)
                     /locus_tag="VF517_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053189.1"
                     /translation="MPPVDLGASAQPPPGAAPPGYGQQAQAGQHGQQGQAGQQPAAWG
                     QPGYGQPGGYGQPGGYGQPGGYTQSGGHGQPAGYGQQSGGYAQQPAGYNQPVYGQQHN
                     PYVHQSPWASTYTYAYNEPDNSPALAGFICSLTSIGTLVVFFGLLSPVTLAVSIAGIV
                     ASRRGIKKVDRGETRKYKALAQWGFWLGIVGTVLSVLALAGWIALIASDPDAFDEEFD
                     PDNGDPARALAPAAVALARAAAALLA"
     gene            complement(9760..10548)
                     /locus_tag="VF517_09370"
     CDS             complement(9760..10548)
                     /locus_tag="VF517_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053190.1"
                     /translation="MTSAQPPEGGGWDPPTEGGGWDPPAPGGGTPGGGSEQPTQGGSA
                     PPTQGGEQPAQGLPPGWGQQGYGQQQGYGQQAGYGQQAGYGQQPYGQQGWGQAGYGQQ
                     QPGYGQQPGYGAPPYGQQYGYGPYYGYPSYEAYVTATQGPGNSAAVGGFIASIASLGL
                     LVTFAGFSAPLTIFGSIAGAIVSRNGVKKVDRGETQKQRDLGQWGFWLGIAGIVLAVI
                     AVAVWAAIFLAAGSTDDGYDSYGRIHDITRVAARVAHAAVPLLS"
     gene            complement(10621..11124)
                     /locus_tag="VF517_09375"
     CDS             complement(10621..11124)
                     /locus_tag="VF517_09375"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX8053191.1"
                     /translation="MPGGPQSGPPPGPAPGYAQPGPGPAPAGYGAPGQMVSPGMAPPI
                     LQGREFAGWWQRVGASLIDTIVIIVGLIILGYLLQAYMMGREGEKNGMTLGKQALGIK
                     ALREDGQPWTFGTGLFREFVIKYLLVGVVGGFIIVGSLINYLWPLWDERKQALHDKII
                     KDYVVKV"
     gene            complement(11260..>11868)
                     /locus_tag="VF517_09380"
     CDS             complement(11260..>11868)
                     /locus_tag="VF517_09380"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HEX8053192.1"
                     /translation="EPAPVPAAPQADWGSPPPPPMGAPPPPAGGPPPPGAGQGFGSPQ
                     QQGYGQPPQAYGEPQQAYGQPQYGQPGGAMVGPGQGVTPGLQPQDSTRVWMLSVVTFG
                     IYALIWFHRTNTGLQEWSRGRIEYNAGASLSAMTIGAVLFVPPLVATASYMGRIRTAQ
                     QMAGLPPQAGFWGYVLRNYLLFYGIKWAQDQINEIAVRQPQG"
BASE COUNT         1579 a         4105 c         4451 g         1733 t
ORIGIN      
        1 gccgggcgcg gcgatgtccg ggttcatccc gaggcacatc gagcagccgg ccgagcgcca
       61 gtcgaagcca gccgcgcgga agacctcgtc gagcccctcc tgctcggcct gcgccttcac
      121 ctgctgcgat ccggggacga ccatggcgtg caggtcagcg ctgaccttgc ggcccttgac
      181 cacctcggca gccgcgcgca ggtcgccgat ccggctgttg gtgcacgagc cgatgaagat
      241 ccggtcgaga cggatgtcct gaatcgccgt gccgggcgcg aggtccatgt agcgcagggc
      301 gcgctcctcg ccctccgtgc tcggctcggg aacggcggcg gtcaccgggg cgaccatctc
      361 cggtgtggtg ccccatgtga cctggggctc gagcgccgtg gcgtccacct cgatctgctt
      421 gtcgaagctc gcgccgtcgt cggtgcgcag cgcgcgccac tcgtccaccg gaagctggtc
      481 gggcgccgcg gggcggccct cgatccactc gaacgtggtg tcgtcggggg cgatcatgcc
      541 ggcccggccg ccgccctcga tcgtcatgtt gcagatcgtc atccggccct ccatcgagag
      601 cgtctcgacg gcctcgccgg cgaattcgat cgcgtgcccg acgccgcccg agacgcccat
      661 ctggccaatc gtgccgagga tgaggtcctt ggcggtcacg ccgaagccga gcgtgcccgt
      721 gtagaggatg cgcatggagc gcggcttggg ctggaccatg gtctgggtgg cgagcacgtg
      781 ctcgacctcg ctcgtgccga tgccgaacgc gagcgccccg aaggcgccgt gggtggcggt
      841 gtgcgaatcg ccgcagacga cggtcatgcc gggctgcgtg atgccgagct ccggcccgat
      901 cacgtggacg atgccctggt gggcggagcc gagcgagtag tgcggcacgc cgaactcgcg
      961 gcagttgcgc tcgagcgtct cgacctggac gcgggagagc tggtcgcgga tctgcgcggc
     1021 ggtggtggag ccgtcggtgg gcacgttgtg gtccgcggtg gcgagggtgc ggtccggacg
     1081 gcgcaccttg cggcctgcga gccggagcga ttcgaacgcc tgcggcgagg tgacctcgtg
     1141 gaccatgtgc aggtcgatgt agatgaggcc cggcgcgacc tcgtgggctt cccagatctt
     1201 gtcgaacatc gtcttggcca tcgggctcag ctccttctca gtccggccgt gacgacggcc
     1261 atgaagcggg cctcgtcggg gcccgggttc tcgaagcggt gcgggaggtc ggcgtcgaag
     1321 gtgacggcgt ccccctcgtt cagctcgtac ctcgtgccag tgcagatcaa agtcaaacga
     1381 ccagcaatga ggacagcggt ttcgcggctt cccggctcgt ggatcggcgg gtcgtcaggc
     1441 ccgccagtcg ccgcgccggg cttcagcacg tggcgcgaga cctcggcccg ctggccgggg
     1501 agctgcgggg tgagcagctc gtaggcgtgg ccgctggccc tcgatgcccc ggggcggcgc
     1561 tggtggcggc ggatgaccgt gacgccctcg ctctcgtcga ggcgcaggag ctggctcagg
     1621 gagagctcga ggccactggc gatgcggttc gcgacggcga gcgtagggct cgtctcgccg
     1681 cgctcgacct ggccgatcat gggggcgctg acgcccgccc tctcccctag gtccctgagc
     1741 gacaggccca tggcctcgcg cagggcgcgg atgcgcgggc ctatctcgac cgcggcgctg
     1801 ggaacggcat cggtcagggg ggcggttgcg gttgccatcc gagtgtacgt tatcacgtac
     1861 ggttgtacgt catccggtct cgccttaatg aggtgcccgg ctttagtggc gtatagcgcc
     1921 acaaactccg ggcacctcgt tcagagggct tcatgcagga gggcgcggag ctcgtcggcg
     1981 cccggtggat cgggcgtgtt ggcgaggtcc gcgcgctgca gggcctcctc caccaccgcg
     2041 tcgatctggt cctcggagac gccgatttcg cccaggcggc gcgggcccgc gagggcggcg
     2101 agctccgcca cgcgcgccgg gaccgcggcg gggttgcggg tgccgaggcc gagcgcctcg
     2161 ccgagcttcg agatcgcagg ggtggcgcgc tcgagcatcg cctcgagcgt gtacgggagg
     2221 aggaccgcgt aggcggcgga gtgggagacg ccggtgacgc gcacgagcgt ctggccgagc
     2281 aggtggtgca gggcgaagcc ggcggagccg accgcataac ccgcgaggag agagccgaga
     2341 gccagctcca accgaacctt gtcctcattg ccgccgttgc cgtcctccga ggagagatcc
     2401 agcccgcccg acaggtgctc cgccgcgcgc agggccgcca tctccgcgac ggggttggcc
     2461 atcgggccgt agagcgcctc ggccgcgtgg gccagcgcgt tcatggcgct ggccgcccgc
     2521 ggctgcggat cctgcgagga catcaggtcg gggtcggcga tcacgagcga cgggcggacg
     2581 aggttccacg cgtccacgcc cgcgggcatg cggtggatcc tcgtcatggg cgcgccgctc
     2641 agggttgttg ggaccgcggt cacagagagg gaatcggcgc ccgcgatgcc cttggccgcg
     2701 tcgatgaccc gcccgccgcc gagggcgacg aggggcaggc ccggcgggag cgcctcgcgc
     2761 accgtggcgg cggcctcggg cacgggcccg gccggcacat gcacgacggc cgaggcccgt
     2821 tccccaagtg atggcagttg ccgttccgcc cgctcagtgg tgaccagagc gaacccgggc
     2881 tcgagcgaac cgacgtccgc cggcccgaag acgatcgtcc gctcgccgtc gagccagcgg
     2941 aacgtcaagg cgtgacggct tctgacggag cctcggaggg gatgtcggcg gccagcgcgt
     3001 tcgacagcgc gcgcaggtaa gcctgacccg aagcctcgag gatgtccgtc gagacacccg
     3061 cgcccgaggc gagcctgccc ccgagctcga gcagcacgct cacctcgccg agcgcgtcgc
     3121 gcccgccggt gaccgcgctc acgtggtact cgcgcagccg cgcctcgtgg ccggtggccg
     3181 cgttcagcgc gctgaagaac gcgtcgaccg ggccgtcgcc cgtgaagctg ccctcggcaa
     3241 gatcgccgtt cggcagtcgc acgatcaccg tcgcgagcgg cgcgcgctgc gacgaggcct
     3301 ccacctcgaa cgactggagc ccgaacgcca ccggcgactg gcgcatctcg tcggagacga
     3361 tcgcctcgag gtcgagcgcc gtgaccttct tcttcttgtc ggcgatgtcc ttgaagcggc
     3421 ggaacgccgt gttcagcgcc gcgccctcca cctggaaccc gagctcctcg agcgccttgc
     3481 gcagcgcgtg gcgtccggag tgcttgccga gcacgatcga gttcgcctcg aggccgatcg
     3541 tctcggcgtc catgatctcg taggtcgtgc gctccttcag cacgccgtcc tggtggatgc
     3601 cggcctcgtg cgcgaacgcg ttgcggccca cgatggcctt gttcggctga accgcgtagc
     3661 cggtcagccg cgagatcatg cgcgaggtgc gcgcgatctc gcgcgtctcg gccccggtcc
     3721 acagccccac gctcgactcg cgcgtgtgga gcagcatcac gatctcctcg agcgacgcgt
     3781 tgcccgcgcg ctcgccgatg ccgttgatcg cgcactcgac ctggcggcag ccggcggtga
     3841 ggccagcgaa ggagttcgcg acggcgagcc cgaggtcgtt gtggcagtgg accgagacga
     3901 ccacgtcgcg caggtcgggc acgagccggt agagctcctc gaacatcgcg gcgtactcgt
     3961 gcggcatcgt gtagcccacc gtgtcgggca cgttgatcgt ggtcgcgccc tcgtcgatcg
     4021 cgatctggat cacctcggcc atgaactcga cgtccgagcg cgagccgtcc tcgggcgaga
     4081 actcgacgtc gtcggtgtac tgcttcgcgt gcgcgaccgc cgcgcgggcc tggcccttca
     4141 cgtcctcgcg cgtggtctgg agcttgcgct cgatgtggat gtcgctggtc gcgatgaacg
     4201 tatggatgcg cggacgctcg gagtcctgga tcgcgctcca ggcggcgtcg atgtcctgga
     4261 acgacgtgcg cgcgaggccg cagatgatcg gaccgtggac ctcgcgggcg atcgcctgga
     4321 cggcctcgaa gtcgccgggc gaggtgatcg ggaagccggc ctcgatgacg tcgacgccga
     4381 gacgcgcgag ctggtgggcg atctccacct tctcctgctt gttgagcgag atcccagggg
     4441 actgctcgcc gtcgcgcagc gtcgtgtcga agatccggac ccgatcctcg tcgcgcctct
     4501 cgtgcctgcc tacgtcgctc tccatgtcgt tcctttcagt gctctccgaa gttctcctgt
     4561 ggtcatgcgg cttggcggcc gccacgcgtt attccccccg aggggggagc aggagaagtc
     4621 gtagggagag cacgttcgac atgcgatttc ccactctaca gggtgatcgc aaccgcgccg
     4681 gcgatcagtc gcccgcgccg ccctgcacga cgaagtgcat ctctcgctcc gaggggaccc
     4741 gggagcggcc gaccaccgcg tgcgcgcgca ggtcgatcac gtgcaccgtg tcgccggcgc
     4801 gggcgtaggc gtggtagccc agcacctgaa cgtcgtggac gacctcgccg gcgagcatcc
     4861 ggaagcgcgg gcggccctgc cggtcgaacg cgcgcagtcc ctccgagccg ccgggcgacc
     4921 cggcgctgcg gccgtcgtag gtgagcacca ggccgggggc gagccgcacg gcgccagcgc
     4981 cgcggtcgac caggcgccgg gtgccggaac gcgggtcgac gacctcgacc cccttcggca
     5041 ggccggcgcg aatcgcggtg cgccgagagg agccgccggc cgtcgatgcc gaccacacgc
     5101 gtggccgcga cgcgctcggg ggcggagccg cgaaccggtc ggtacgtgac gcgcatcgtc
     5161 ctgaggtcga catcggccac ccgcgggccg gggccggcga tctgcgcgcg atcgcgcgag
     5221 gagtcgacgg cgaggccgag gcgcccgcac tccggcgcca tcgcggcgag cgtcgtgatt
     5281 cgagtccgcc cgcgcttgtc gaccgtgacg agcctgtttc cgagcagcat cacgagcagg
     5341 cggccggtcg tggccttgcc gggcgggtcg atgcagggcc cgggcacgcg gccgggacgg
     5401 ctgaggacac ggcccgtgga cggcttcacc acgaccacct ctcccgcctg ggtcacgccg
     5461 atcaggcggc gcggagcgag gtaggcgagc gccatggtgg cgaccccgac ctgcacgtcg
     5521 gtcgtgaccc gcatcgcgtc gaggtccacg atccggatcc cgccgcggcc gccgctcggc
     5581 acgccgttga ccggggtgcc ggtcgagatc gtgaacgcgg cctgcccgcc gtcggaagag
     5641 agcgcgtagc cgtcgtgcca ctcggggatc tcgatggccg ggccctctgg cgcgagcgtc
     5701 agcgggtcga gccggctgag ctcggtgacc ccgccgctcg ttccgtacat gcctagcgga
     5761 gcgaagaacg gcggctgcgg ctcggcggcg ggcgtggcgg ccggccaggc gaggagagcc
     5821 agcgagcaga ggatgagcgc aagcggctgt ctcatcgtct gtgggaacgc gccacggcgc
     5881 gcggagttgc ggcctagaga cttacggcgc gaccgtcgtg gaagccgtcg cccgcgacga
     5941 tctcccgcat gacgtcgtcc gggacaggac ggtccgtggt cacgaccatg atcgcctcgt
     6001 cgccggtgct cggctcgtcg tcgagcccgt ggcctacggc ggccgagtcc acgttcacgt
     6061 ccgccgcgcc gagcgccgag ccgacccgcc cgatcatgcc cggctggtcg cggtagcgga
     6121 acaccgcgag gtggtcctcg agctgcacgt tgaagcgcga tccccacgcc tcgagcaggt
     6181 ggccgcggtg gcggttgccg agggtggtgc caaccacgcg actgcggttg ccgccggtga
     6241 ccaccgtgac gcgcacgagg tcggtgaagt cgcgcgccga cggctgcttg gtctcggaga
     6301 cgacgatgcc gcgctcggac gcgagcgtgg aggcgttcac gtcgttgacg ggctcctcgg
     6361 tcttgccctg gaggatcccc ttcagcacct gcaccgtgag caggcgcgtg tcacggccgg
     6421 cgatcccgcc gaggtactcg gcctcgacgg cgtcgacgga gccgttctcg gcgaggccgg
     6481 ccgcgatccg cccgaggttc gcgcacagcg gcaggaacgg gccgagctcc tcgaggtccg
     6541 cggccgagac ggccgggacg ttcaccgcgc tcgtgacgac gccgccggtg agggcggcga
     6601 ccacctgctc ggcggcctgg tagcccgcgc ggtcggtggc ctcggcggtg gaggcgccga
     6661 ggtgcggggt gacgatcacg ttcgggtacg cgaacagcgg atggtcggtg atcggctcct
     6721 cctggaagac atcgagcgcc gctccggcga ccttgccgga gtcgagcgcc gccttgaggt
     6781 cggcgtcgac gagcagcggc ccgcgggcga cgttgaggac gcggacgccg tccttcatct
     6841 tcgcgaacgc ctcggcgttg agccagccct cggtctcggg cgtcttgggg aggtggatcg
     6901 tgacgaagtc ggccgccgcg tagagctcgt cggaggtctc ggcgcgctgc acgcccatgt
     6961 cgcggaagcg ctcctcggag acgtagaggt cgaaggcgac cacgcgcatg ccgaacccct
     7021 gcgcgcgctc cgccacgagc tggccgatgc ggccgaaccc gatcacgccg agcgtcttct
     7081 cgtagagctc gacgccgctg aacttcgagc gctcccactt gcccgaggtg agcgacgcgt
     7141 gcgcctgcgg caggttgcgc gcaagcgcga gcagcagcgc gagcgtgtgc tcggcggcgg
     7201 tgatgacgtt cgactggggc gcgttcgcca cgacgatgcc gcgcttggtg gcggccggca
     7261 cgtcgacgtt gtccacgccg acaccggcgc ggcccacggc ccggaggcgg tcggccttcg
     7321 cgagcagctc ggccgtgagc ttcgtggccg agcggatcag gatgccgtcg tagtcgccga
     7381 tccgctgctc gagctcgccg tcgtcccagt cgaccccgag ctcgacgtcg aagccggcgt
     7441 cgcgcaggag ctgcacgccg gactcgccga tcttctcctt tacgagtact tgcggagcgg
     7501 cgttcacgcc gccacgggca cgccggcgtc gaggaagacg ccctgcgccg cacccacgcc
     7561 cgcaccgagc tcgaggtcgt gcccgagctc gcggagggtc atctcgaacc ctgagagcgt
     7621 ggtgaggatg tcgaacgcgc cgaagtagcc gcagtgcgcg atgcgcgcga tcttgccctt
     7681 gagcttgccc tggccgccgg cgatggtgat gccgtagcga tcgcgcatca gcttcggcac
     7741 cttgccgcca tcgatcgtct cgggcagctt gatcgccgtg gcgacgttcg agttctcgtc
     7801 ctcggggccg tagatctcga ggtcgagcgc cttgacggcg ctgcgggtgg cgcggccgag
     7861 aaggcggtgg cgctcgaaga cctgctcgag gccctcctcc tccatcatcg cgagcgcgac
     7921 gtcgagcgcg atgaacagcg tgaccgccgg ggtgaacggg ctgtccggcg gatcctcgcg
     7981 ctggcccttg gcggtcttct cccagtcgaa gtagtggcgg cggccgggga tcgaggcggc
     8041 gcgctcgagg gcggccgcgt tcgggctcgc gaaggcgagg ccgggcgggc acatgagcgc
     8101 cttctgcgag ccggacacga ccacgtccac tccccactcg tcctgaggga tcgggacggc
     8161 gccgaggccg gatacggcgt cgaccacgat cagggccccg gccgcgtgcg cgacctcggt
     8221 gagcgcctgc acgtcgttga ccacgcctgt cgaggtctcg gagagggtgg tgaagatgac
     8281 cttcacgccc tcgttctcgg cgagccggcg ctcgagctcc gcgggctcga ccttgttgcc
     8341 ccagccggcg tcccagtgga tcgtgcgggc gccgaagtcg tcggccagct cggcccagcg
     8401 ctcgccgaac ttgccgcagc tcgcgacgaa ggccggctcg ccgggctcga cgaggtttgc
     8461 gacggcggac tccatcgcgc cggagccgga ggcggcgaag gtgagcacgt cgttctcggt
     8521 ctggaagacg cccttgagct tctcgaggca gcgcgcgtag acctcgatga acgcgggcgc
     8581 gcggtggtag aggatcggct ccgccatggc gttcgagacc gcgggcggga gcggcgtcgg
     8641 gccggcggtc atcacgtagc tcttgatgta cgggttggcc atcgagcggc gagaataccg
     8701 aaggggtcgg gggcctcttt ggcgccgggc cggagggccg cggcgggggt cggcggcgcg
     8761 cgcgaggagc ggcgcggcgg cccgtgtgag cgcgagcgcg gtggtcaggc gaggagcgcc
     8821 gcggcggccc gtgtgagcgc gagcgcgggg tcaggcgagg agcgctgcgg tggtcaggcg
     8881 aagagcgcgc cgcggggtca ggcgaggagc gccgcggcgg tcaggcgagg agcgctgcgg
     8941 cggctcgcgc gagcgcgacc gccgcgggcg cgagcgcgcg ggcggggtcg ccgttgtccg
     9001 ggtcgaactc ctcgtcgaac gcgtctgggt cggaggcgat cagcgcgatc cagccggcca
     9061 gcgccagcac cgagaggacg gtgccgacga ttccgagcca gaagccccac tgcgcgaggg
     9121 ccttgtactt gcgggtctcc cccctgtcga ccttcttgat gccccgtcgc gaggcgacga
     9181 tcccggcgat cgagacggcg agggtcacgg gcgagagcag gccgaagaag acgaccagcg
     9241 tgccgatcga ggtgagcgag cagatgaaac cggcgagcgc gggcgagttg tcgggctcgt
     9301 tgtaggcgta ggtgtaggtc gaggcccacg gggactgatg gacgtacggg ttgtgctgct
     9361 ggccgtagac gggctggttg tagccggcgg gttgctgggc gtagccgccg gactgctgcc
     9421 cgtaaccggc gggctgtccg tggccgccgg actgggtgta gccaccaggt tgcccgtagc
     9481 cgccgggctg gccgtagccg ccgggctggc cgtagccggg ctggccccag gcggccggct
     9541 gctggccggc ttggccctgc tggccgtgct ggccggcttg ggcctgctgc ccgtagccgg
     9601 gcggggccgc acccgggggc ggctgcgccg aggcaccgag gtccactggc ggcatcggcg
     9661 cgcctccgga tgtggcgtcg gtggcggaat ctccgctcga gcccggcgcc tgcgggggaa
     9721 gccacccgcc ggcgggcggg ccctgcgggt ccggcgtcat caggagagca gcggtaccgc
     9781 cgcgtgcgcc acccgcgccg cgacccgcgt gatgtcgtga atccggccgt aggagtcgta
     9841 gccgtcgtcg gtgctacccg cggcgaggaa gatcgctgcc cacacggcga ccgcgatcac
     9901 cgcgaggacg atccccgcga tgccgagcca gaagccccac tggccgaggt cgcgctgctt
     9961 ctgggtctcg ccgcggtcga ccttcttgac gccgttgcgc gaaacgatcg cgcccgcgat
    10021 cgagccgaag atcgtcagcg gggccgagaa gccggcgaac gtgaccagca gccccagcga
    10081 cgcgatcgag gcgatgaagc cgccgaccgc cgcactgttg ccgggcccct gcgtcgcggt
    10141 gacgtaggcc tcgtaggacg ggtagccgta gtaggggccg tacccgtact gctggccgta
    10201 cgggggagcg ccgtagcccg gctgctggcc gtagccgggc tgctgctgcc cgtacccggc
    10261 ctggccccag ccctgctggc cgtacggctg ctggccgtac cccgcctgtt ggccgtaccc
    10321 cgcctgttgg ccgtagccct gctgctggcc gtagccctgc tgcccccaac ccggcggcag
    10381 cccctgcgcc ggttgctccc cgccctgcgt cggcggcgcc gaaccgccct gcgtcggttg
    10441 ctccgagccg ccgcccggcg ttcctccgcc cggcgccggc ggatcccagc ctccgccctc
    10501 cgtgggtgga tcccagccgc cgccctcagg tggctgtgcg gacgtcataa gcgcgagcct
    10561 agacgactcg gggccgcgat ccatgccgga cgcgaccccg agtcaagccg ttccaggccg
    10621 tcagaccttg acgacgtagt ccttgatgat cttgtcgtgc agggcctgct tgcgctcgtc
    10681 ccacagcggc cacaggtagt tgatcagcga cccgacgatg atgaagccgc cgaccacgcc
    10741 cacgagcagg tacttgatga cgaactcgcg gaagagtccg gtgccgaagg tccacggctg
    10801 gccgtcctcg cgcagggcct tgatgccgag cgcctgctta cccagcgtca tcccgttctt
    10861 ctcgccctcg cgacccatca tgtaggcctg gagcaggtag ccgaggatga tcagcccgac
    10921 gatgatgacg atcgtgtcga tcagcgacgc gccgacgcgc tgccaccagc cggcgaactc
    10981 gcgcccctga aggatgggcg gcgccatgcc cggcgagacc atctggccgg gcgcgccgta
    11041 gcccgcgggg gccggccccg ggccgggctg cgcgtagccg ggcgccggtc cgggcggagg
    11101 gcccgactgc gggccgcccg gcatgggcgg aggcgggggc ggcgggccgc taccggcggg
    11161 cggcgggccg ctaccggcgg gcggcgggcc acccggagac tgcgggccgg aacccggctg
    11221 ctcgggcggt ccgcccggtg attcagtgct cattcgtgtt cacccctgcg gctgtctaac
    11281 cgcaatctcg ttgatttgat cctgggccca cttgatgccg tagaagagga ggtagttccg
    11341 gagcacgtag ccccagaacc cggcctgcgg cgggagcccc gccatctgct gagcggtccg
    11401 gatccggccc atgtagctcg cggtcgccac cagcggcggc acgaacagga cggctccgat
    11461 cgtcatcgcg ctgagcgagg cgccggcgtt gtactcgatc cgtccgcgtg accactcctg
    11521 gaggcccgtg ttggtgcggt ggaaccagat gagcgcgtag atgccgaagg tgacgacgct
    11581 cagcatccag acccgcgtcg agtcctgcgg ctggaggccg ggcgtgacac cctgccccgg
    11641 cccgaccatc gcgccgccgg gctgcccgta ctgcggctgc ccgtaggcct gctgcggctc
    11701 tccataagcc tgcggcggct ggccgtagcc ctgctgctgc ggcgagccga acccctggcc
    11761 ggcgcccggg ggcggcggac cgcccgcggg aggcggcgga gcgcccatcg gaggtggcgg
    11821 ggggctgccc cagtccgcct gcggggcggc ggggacgggc gcgggctc
//
LOCUS       DASVPL010000183        14380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_178247, whole genome shotgun sequence.
ACCESSION   DASVPL010000183 DASVPL010000000
VERSION     DASVPL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14380
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178247"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1536)
                     /locus_tag="VF517_09385"
     CDS             complement(1..1536)
                     /locus_tag="VF517_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8053193.1"
                     /translation="MTKLEGSNTSIWVDTTPTTSYPALDREVHVDVAVLGGGMAGLMA
                     ALFLKRDGARVAVLEAGRVAQGVTAYTTAKVSSLHGIHYQSVASSFGDDGARAYGQAN
                     EAAIERIEALVEELGIDCDWRRKPAYVYAEDDRGREKVQDEVQAALKAGLKASFTTET
                     DLPWEVTGAIRVEDQAEFHPRKFLLAIAEQIPGEGSHVFENTMARDVKDGEPARVETD
                     QGQIVVADHVIVATHFPFLDRGGYFARMHPERSYALGLYVSGHAPQGMYLSTESPSHT
                     VRSHPTERGELVIAGGESHKVGQSGEDTADRVARLEDWARERFDVTEIAYRWSTQDNM
                     PVDGVPYIGKYWPGATRLWVATGFKKWGLTNGVAAAEILADLIGGRTNPWASLFDPNR
                     FKPAAQAKELVKENANVGWRFVRDKVGGGDFSSLDEIEPGQGGLVREGAGKVAAYRDE
                     QGELHVLSPTCTHMGCTVKWNTAERSWDCPCHGSRFHYDGTVLQGPALDGLSVKGSGS
                     RST"
     gene            1589..2452
                     /locus_tag="VF517_09390"
     CDS             1589..2452
                     /locus_tag="VF517_09390"
                     /inference="COORDINATES: protein motif:HMM:NF005878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8053194.1"
                     /translation="MGELDARVVAVTGGARGIGRAIAGRLASAGARVGIGDLDLATAE
                     ATAAELPGEVAAFQLDVTDRGSMDRFVAGVEERYGPLDVFVNNAGILLVGPFLEEDDA
                     ATQRQFAVNVMGVVHGMRAALPGMRERGRGHVVNVASSASWVAPPGEVTYSATKHAVL
                     ALTDGVREELHGSGIHLTGVFPGLVDTELAKGTAPPRGSRWISPDDVGVAVLKAVITP
                     RPDVFAPREFAFTTRLARVLPTRARHRLMQSLDVGRVTATTSPAERAEYMRRIGLDPR
                     AAGHGTSRGDG"
     gene            2445..3413
                     /locus_tag="VF517_09395"
     CDS             2445..3413
                     /locus_tag="VF517_09395"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8053195.1"
                     /translation="MADGHVFLTGALGFIGCAIADRYAAEGWAVRGVDLTADPERGVV
                     AGDVTRPGDWQDHAAGCEVVIHLAARIGLGGTHDEFWPVNVLATRLALDAAVRGGARR
                     FVHTSSVVTFGLDFPDGVAESYPVRPTGAPYTDTKIASEQVVLAAHLSGEVPCTIVRP
                     GDVYGPRSRPWVLLILEAIRARQFALPARGRGIFSPVYVDNLVDGYVLAASAPAAEGQ
                     VFTISDGVGIECREFFAHHHRWLGVSGPRTVPTGVALLLAGVNDRARRIAGRETEVNP
                     GAVRYFTRPGTYSIEKARTLLGYEPGVGLDEGLERTRAWVVEAGLA"
     gene            3410..3895
                     /locus_tag="VF517_09400"
     CDS             3410..3895
                     /locus_tag="VF517_09400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX8053196.1"
                     /translation="MSAAILEGLFREAMAGLCSGVAVVTARRADGQPCGLVATSVSSY
                     SAAPPSVLASIGHNSRCHPALVEGEHFGVHLLNAGQSELAALFASRSTDKFAGLEWSW
                     DGDVPSLGGVLAYLRCRRSALFERYDHSILIGDVTGGRRGSGEPLVYMDRRMDWRLSP
                     P"
     gene            3917..5158
                     /locus_tag="VF517_09405"
     CDS             3917..5158
                     /locus_tag="VF517_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8053197.1"
                     /translation="MEAGKPDAGKQATQPLDPITRDTADGEPAVRIRPTIDTKSAFRT
                     VAVVLAAAAGIWVIYLLRQPLSWLFIAGFIAIAVSGPVNILSRRMKRGLAIMLVYFGI
                     LLVPVALLALLVPPIVDQVNTLVNDAPQYADDVTEFVNENERLRDLNADYDITTKLQD
                     EAEKLPGKVGDAAGVLADIGIGLVNGIFAGVTILILSIFMVGAGPRWRRTFIKVQAHD
                     PERAEAWNRMFDRIGAAVGNYVLGALIQATIAGVSAYLVLLLIGAPSPAALAVVVFLL
                     DLIPLIGATIGAVVVGVVTLFGDLPIDPIIWTVYSIIYQQIENNVIQPRIQARAVQLE
                     PFLVIVSVLFGSALFGLAGALLAIPVAASIQIAVREYLLFRHDPLAALIAEPKATDPE
                     GEPPEREQPSAGRADAPPEPA"
     gene            5385..6170
                     /locus_tag="VF517_09410"
     CDS             5385..6170
                     /locus_tag="VF517_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX8053198.1"
                     /translation="MESPTAADRRYLQPAPERTRADYALLMRYRQNKDAAARDELVER
                     FLPLARQLARRYARPNEPIDDLFQVASMGLLKAIERFDPARGNAFSTFAVPTIVGELK
                     RYFRDTGWAVHVPRPIQERIGQVNRAVNDLSRELGRSPTPTELSAKLGHSTEEILEAL
                     EAAKAFDAVSLDMPRGNGDDSSAYADTVGAHDERFEMVEYNAVIEPTMAALPDRDRLI
                     LRLRFERDLTQSEIADRLGISQMHVSRIIRRSLDRLRTVAEAA"
     gene            6175..6921
                     /locus_tag="VF517_09415"
     CDS             6175..6921
                     /locus_tag="VF517_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HEX8053199.1"
                     /translation="MVLRRDADGDGALAIGQPSHAWVSGQLARAWGDELDPHEEVCLA
                     AEQHDIGMAEWDLRPTLNRDTGLPHSFMEMPLDTHLELWSRAPAKLLRQSAYAALLVS
                     MHGTALYEMRDLDRMDAGDADKVRRFLAESRTEQDALLAELGQERAGVLVNQRLVWTW
                     DFMSLALCLDWAPTVAERVPLGGALVDMRLEPVGPSALAIDPWPFRAPSVTVRCEGRA
                     LGGGYRDERELHAALAAAPPVPVQWRLVPA"
     gene            6931..9039
                     /locus_tag="VF517_09420"
     CDS             6931..9039
                     /locus_tag="VF517_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053200.1"
                     /translation="MRGALMLVVAVLVAAVAGAPAAAAEFTPDGEAANYAKIRERAQY
                     EHGTPEYRALMQERGIESELELNDIRLNDPERDMSGNLCRSHYDGCAGDIRLWPKHWA
                     AEGHGITRPVLFTARNGAVLSGHVWATRSGPALRPAVVITSGSIQAPEELYVFAATTL
                     AKAGYVVLTYDVQGQGRSDTYGEGVDRDEGFPSQAGQPFYDGTEDALDFMLSTPASPY
                     RPRRSCSTGTSHEEKQLRRVRAGLNAAYNPLSELVDPRRVGVAGHSLGAAAVSYVGQL
                     DARVKAIVAWDNLRDSSAGIAASACPSGASPRPERIALTKPALGMSNDYSLTPTPYSA
                     APDEQAKNAASNGYSAAGVDSAQLNIRGGTHFEYAYIPNPGFGATLRGMDMAAWYMQA
                     WFDRYVKGGVDEDAERRLRTTRWRADARGAEVDPAGDGNLFSHYLKSRLAIGGFRCED
                     MRTGCAGLSADDGRPPNYSYYDVAKTADDGAVTPAAADFDGTAEVTVTKAAETSGADG
                     SFPVRVRAVARREEADHFELQVSRYSPRRAGAYRTIAGQLGAAPHVFRGALGATYRFR
                     ARAVSRGGAAGPWSRAAITMVPFDAAPGRVKFGRGWSSVSDARAWNGSFRRTTRAGAT
                     MAFRWRGSILHLVGRRSPAGGRAWLYVDGRRRSISFRSRKVRNRTLIWAEKVSGRGTH
                     TMRIVTRGGRAEVDGIGVAP"
     gene            9264..10820
                     /locus_tag="VF517_09425"
     CDS             9264..10820
                     /locus_tag="VF517_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8053201.1"
                     /translation="MPDRDDLAEALARRAATRDEARPEAVERRHAGGGRTARENIADL
                     VDPGSFVEYGALAIAAQRARRELQELIERTPADGLVAGTARVNEDLFGERSACAVLSY
                     DYTVLAGTQGVLGHRKKDRLFELIERMRLPTVFYAEGGGGRPGDTDYPVVSALDTRAF
                     ALWARLSGLVPRIAVVAGRCFAGNAVIAGCSDLIVATENASIGMGGPAMIEGGGLGRV
                     DPDDVGPVDMQAGNGVIDVVAADEAEATAVTKRLLAYFQGPTPPGEEPDQTVLRDIVP
                     QRRRRAYQVQPVIETLADAGSVTFLRERWAREMVTALVRIEGRPVGIVANNTMHMAGA
                     VTSEGADKAARFMQLCDAFGLPLVSLVDTPGMMVGPTAEATGLVRHCSRLLVTGASLR
                     VPLVAVVLRRGYGLGAQAMVAGSLHEPLLTVAWPTAELGPMGLEGAVRLGFRRELETI
                     ADEAERNQRVRELTELAEQNAKAVNAATLFELDDVIDPAETRRLIASTFAAAAPAAAA
                     EPPRGFVDTW"
     gene            complement(10821..11714)
                     /locus_tag="VF517_09430"
     CDS             complement(10821..11714)
                     /locus_tag="VF517_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053202.1"
                     /translation="MVGGLIVDPAAPAALRDSGSYAWAVASAWRGDLMLLARTLRAAV
                     LAITRADGAVPGCFVEIAVRDDVELFRSPADLKEWATPRAALRFDAIRVAVRGTATDL
                     DLRIARGRSTAITLPDIPGVMLEVGSTASDGRAIKVLNDVRPSVGRAGFRRTLARGRI
                     PLNPEPITGTDPAVRDRTLHERVERRIGRFAWPYLALTYGITAVFVAASDALAGTHFF
                     DTPWSLLIVVFAPLPVLYGLLAVAQRDDLPIGPRTYALLFPAVELAEVTVGRRIARFA
                     GRLALAAPVASIGVLVNAALT"
     gene            complement(11819..12817)
                     /locus_tag="VF517_09435"
     CDS             complement(11819..12817)
                     /locus_tag="VF517_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053203.1"
                     /translation="MEPREREDGRMEHSGRKLARCAVAAAIAALAVAAPVSANERAEL
                     EVGFTTARPGASTGLTLAVEYKNPSDPSAKPPPLASAAFELPSGTRIDNAALPQCTAT
                     DAEIHARGRNACPPETKIGEGRLVAHTGTPIDPFDGEGVFFNGAGEIIEIVVVRGTDE
                     AAGFDRIGIEGSTLRAHPPALPGGPPDGRTSIKRVELTAPARVEGGRVYVTTPPDCPA
                     DGLWRAAGRFSFDDGGSTTVFDSLSCERPAAPRLKLRVSPRTVAAGGRATLRVRVSSA
                     TPGCAAGASVRLGRARATTDSAGRAVLRKKLSKPAAVTVRVAKPGCGRATARVTVR"
     gene            12865..13575
                     /locus_tag="VF517_09440"
     CDS             12865..13575
                     /locus_tag="VF517_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX8053204.1"
                     /translation="MTPRTSSGLTRAAVAHAALQRLDAEGLDALSMRRLAGELGVGTM
                     TLYGYFRSKEELLDAVVDAGFSEFEMPPAGDDFRTTLRDFALAARAVLVRHPALVEIR
                     DAGPILRPRGFQVTELGVRILSEAGLGAEDAARSFRLLFDYVFGYAIVNRRPPSPELR
                     REALASIVALPPEEFPTVTAVAEQMAEAVGAEGQFEYGLERILDGIEARLAASRAGRR
                     ARPGAPGARRRGTGPRPR"
     gene            complement(13499..14329)
                     /locus_tag="VF517_09445"
     CDS             complement(13499..14329)
                     /locus_tag="VF517_09445"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX8053205.1"
                     /translation="MALTVGRGPFGHTPAGTFNRELPPLKGLIYFEDFPRRIRARFAG
                     ETVVDSCRAKLLHEHGLLPILYFPEDEVRTDLFEPSDHATHCPWKGDARHHTLRVGDS
                     VAKDAAWSYPAPLEGAAGLAGHVAFYWDRVDGWLEEDEPLMVHMRDPYHRTDVLDTSR
                     HVRVEIGGETVAESTRARALFETGLPPRWYLPREDVRDDALVPSDTRTGCAYKGYALY
                     HSVRVGETEERDVVWYYPEPLRDAERIRDRLCFLNEVVDLYLDGELQERPVSPFSRRD
                     "
BASE COUNT         1930 a         5162 c         5303 g         1985 t
ORIGIN      
        1 tcatgtgctt cggctacccg agcccttgac ggacaaaccg tcgagcgccg gtccctggag
       61 cacggtgccg tcgtagtgga agcgcgatcc gtggcacggg cagtcccagc tgcgctcggc
      121 cgtgttccac ttgacagtgc agcccatgtg cgtgcaggtg ggcgagagca cgtggagctc
      181 cccctgctcg tcgcggtacg cggccacctt gccggcgccc tcgcgcacga gcccgccctg
      241 cccgggctcg atctcgtcga gcgaggagaa gtcgccgccg ccgaccttgt cgcgtacgaa
      301 gcgccagccg acgttcgcgt tctccttcac gagctccttg gcctgcgcgg cgggcttgaa
      361 gcggttgggg tcgaacagcg aggcccacgg gttggtccgg ccgccgatca ggtcggcgag
      421 gatctcggcg gcggccacgc cgttcgtgag cccccacttc ttgaagccgg tggcgaccca
      481 cagccgcgtc gcgcccggcc agtacttgcc gatgtagggc acgccgtcga ccggcatgtt
      541 gtcctgagtg gaccagcggt aggcgatctc ggtcacgtcg aagcgctcgc gcgcccagtc
      601 ctcgagccgc gccacgcggt cggccgtgtc ctcgccgctc tggccgacct tgtggctctc
      661 gcccccggcg atgaccagct cgccgcgttc cgtcggatgc gagcgcaccg tgtgcgacgg
      721 gctctcggtg ctcaggtaca tgccctgcgg cgcgtgcccg ctcacgtaga ggccgagcgc
      781 gtaggagcgt tcggggtgca tgcgcgcgaa gtagccgccg cgatcgagga acgggaagtg
      841 agtggccacg atcacgtggt cggccaccac gatctggccc tggtcggtct ccacgcgcgc
      901 cggctcgccg tccttgacgt cgcgcgccat cgtgttctcg aagacgtggc tgccctcgcc
      961 ggggatctgc tcggcgatcg ccagcaggaa cttgcggggg tggaactcgg cctggtcctc
     1021 gacgcggatc gcgccggtca cctcccaggg cagatcggtc tccgtggtga agctcgcctt
     1081 cagccccgcc ttcagcgcgg cctggacctc gtcctggacc ttctcgcggc cgcggtcgtc
     1141 ctcggcgtag acgtacgcgg gcttgcgacg ccagtcgcag tcgatcccga gctcctcgac
     1201 gagcgcctcg atccgctcga tcgccgcctc gttcgcctgc ccgtaggcgc gcgcgccgtc
     1261 gtcgccgaag ctggaggcga ccgactggta gtggatcccg tggagcgagc tgaccttggc
     1321 ggtcgtgtag gcggtcactc cctgcgcgac ccgcccggcc tcgagcaccg ccacgcgggc
     1381 gccgtcgcgc ttgaggaaca gagcggccat caggcccgcc atgccgccgc cgagcaccgc
     1441 cacgtccacg tggacctcgc ggtcgagagc gggataggag gtagtcgggg tcgtgtcgac
     1501 ccagatggag gtgttgctgc cttcgagctt ggtcatggtc tgcctacggg acggggccgg
     1561 aagcgggatg aaatagggtg ctacccggat gggggaactc gacgcgcggg tagttgcggt
     1621 gaccggcggt gcgcgcggga tcggacgcgc gatcgcgggg cggctcgcga gcgccggcgc
     1681 tcgcgtcggg atcggggacc tcgacctcgc gaccgccgag gccaccgccg ccgaactccc
     1741 cggtgaggtc gccgcgttcc agctcgacgt gaccgaccgc ggctcgatgg accgcttcgt
     1801 agcgggggtg gaggagcgct acgggccgct cgacgtgttc gttaacaacg ccggcatcct
     1861 cctggtggga ccgttcctgg aggaggacga cgcggccacc cagcgccagt tcgccgtgaa
     1921 cgtgatgggc gtggtccacg ggatgcgcgc ggcgctgccg ggcatgcgcg agcgcggtcg
     1981 cggccacgtg gtgaacgtcg cctcgagcgc cagctgggtg gcgccgcccg gcgaggtgac
     2041 ctactcggcg accaagcacg ccgtcctcgc gctcacggac ggcgtccgcg aggagctgca
     2101 cggctccggc atccatctca ccggggtctt ccccggcctc gtcgacacgg agctcgccaa
     2161 gggcacggcg ccgccgcgcg gcagccgctg gatctccccc gacgacgtgg gcgtcgccgt
     2221 gctgaaggcg gtcatcacgc cgcggccgga cgtgttcgcg ccgcgcgagt tcgcgttcac
     2281 cacgcgcctc gcgcgcgtgc tgccgacgcg agcccgccac cggctgatgc agtcgctcga
     2341 cgtcgggcgg gtaaccgcca ccacctcacc cgccgagcgc gccgagtaca tgcgccgcat
     2401 cggcctcgac ccgcgcgcgg cgggccacgg cacctcgcgc ggcgatggct gacgggcacg
     2461 tcttcctcac gggcgccctc ggcttcatcg ggtgcgcgat agccgaccgc tacgcggcgg
     2521 agggctgggc ggtccgcggc gtggacctga ccgccgatcc cgagcgcggt gtggtcgccg
     2581 gcgacgtgac ccggcccggc gactggcagg accacgccgc cggctgcgag gtggtgatcc
     2641 acctggccgc gcgaatcggc ctcggcggca cccatgacga gttctggccg gtgaacgtgc
     2701 tggccacgcg gctcgcgctc gacgcggcgg tgcgcggggg cgcgcgccgc ttcgtgcaca
     2761 cgtcgtcggt cgtgacgttc gggctcgact tccccgacgg ggtggccgag tcgtaccccg
     2821 tgcgccccac cggcgcgccg tacacggaca cgaagatcgc ctccgagcag gtcgtcctcg
     2881 ccgcgcacct gtccggcgag gtgccgtgca ccatcgtccg ccccggcgac gtctacggcc
     2941 cgcgctcgcg cccgtgggtc ctgctcatcc tcgaggcgat ccgcgcgcgc cagttcgccc
     3001 tccccgcgcg cggacgcggg atcttcagcc ccgtctacgt cgacaacctc gtcgacggct
     3061 acgtgctggc ggcatccgcg ccggccgccg aggggcaggt gttcacgatc agcgacgggg
     3121 tgggcatcga gtgcagggag ttcttcgcgc accaccaccg ctggctcggg gtgagcggcc
     3181 cccgcaccgt gccgaccggc gtggcgctcc tcctcgccgg cgtcaacgac cgcgcacgac
     3241 gtatcgccgg gcgcgagacc gaggtgaacc ccggcgccgt ccgctacttc acccggcccg
     3301 gcacctactc gatcgagaag gcgcgcacgc tgctcggcta cgagccgggc gtggggctgg
     3361 acgagggtct cgagcgcacc cgcgcctggg tcgtcgaggc ggggctggcg tgagcgcggc
     3421 gatcctcgag gggctcttcc gcgaggcgat ggcgggcctc tgctcgggcg tggcggtggt
     3481 caccgcgcgg cgcgcggacg gccagccatg cggcctcgtc gccacctcgg tctcgtcgta
     3541 cagcgccgct ccaccgtcgg tgctggcgtc gatcggccac aactcgcgct gccacccggc
     3601 gctcgtcgag ggagagcact tcggcgtgca cctcctgaac gccgggcaga gcgagctggc
     3661 cgcgctgttc gccagccgct cgaccgacaa gttcgccggg ctggaatgga gctgggacgg
     3721 cgacgtgccg agcctcggcg gcgtgctcgc gtacctgcgc tgccgccgca gcgccctgtt
     3781 cgagcgctac gaccactcga tcctgatcgg ggacgtgacc ggtggccggc gcgggagcgg
     3841 cgaaccgctc gtgtacatgg accgccgcat ggactggcgc ctgagtccgc cctagccgct
     3901 gcgtaggatg cgcggcatgg aggccggcaa gccggacgcg ggcaagcagg cgacccagcc
     3961 ccttgacccg atcacccgcg atacggcgga cggcgagccc gcggtccgca tccggccgac
     4021 gatcgacacg aagtccgcgt tccgcaccgt ggccgtcgtg ctcgccgccg cggcggggat
     4081 ctgggtcata tacctgctgc gccagccgct gagctggctc ttcatcgccg gcttcatcgc
     4141 catcgccgtc tcgggcccag tcaacatcct ctcgcggcgg atgaagcggg gtctcgcgat
     4201 catgctcgtc tacttcggga tcctgctcgt gccggtcgcg ctgctggcgc tcctcgtccc
     4261 gccgatcgtc gaccaggtga acacgctcgt gaacgacgcg ccgcagtacg cggacgacgt
     4321 cacggagttc gtcaacgaga acgagcggct gcgcgacctc aacgccgact acgacatcac
     4381 caccaagctc caggacgagg ccgagaagct gcctggcaag gtgggtgacg ccgccggcgt
     4441 gctcgcggac atcgggatcg gcctagtgaa cggcatcttc gcgggcgtca cgatcctgat
     4501 cctcagcatc ttcatggtgg gagcggggcc gcgctggcgc aggacgttca taaaggtcca
     4561 ggcgcacgac cccgaacgcg cggaggcctg gaaccggatg ttcgaccgga tcggcgcggc
     4621 ggtgggcaac tacgtcctcg gcgccctcat ccaggccacc atcgccggcg tgagcgccta
     4681 cctggtgctg ctcctgatcg gcgcgccgtc gcccgcggcg ctcgcggtcg tcgtgttcct
     4741 gctcgacctg atcccgctga tcggcgccac catcggagcc gtggtcgtgg gggtggtgac
     4801 gctgttcggc gacctcccga tcgacccgat catctggacg gtctactcga tcatctacca
     4861 gcagatcgag aacaacgtga tccagccgcg gatccaggcg cgggcggtgc agctcgaacc
     4921 gttcctggtg atcgtttcgg tgctgttcgg cagcgcgctg ttcggcctcg cgggcgcgct
     4981 gctcgcgatc ccggtcgcgg cctcgatcca gatcgcggtg cgcgagtacc tgctgttccg
     5041 gcacgacccg ctggccgcgc tgatcgccga gccgaaggcg accgatcccg agggcgagcc
     5101 ccccgagcgc gagcagccga gcgccgggcg cgctgacgct ccgccggagc cggcctagcc
     5161 tctccccgac cggggccggc cgcgcgcggt accaccggtc gcgtaggtcc aaaccggaga
     5221 tttcaagggc tggcgctttg ccgccgactg atgtatgaat aagcgagcag ggggcccacc
     5281 atggcccatt cgggactcgc ccaggacgga ctcgtcgccg aacggccgga ggtacgccgg
     5341 tcgaggtcgc ggcgtgcgct caaacgagaa gagctttgct gcaaatggaa tccccaaccg
     5401 ccgccgatcg caggtacctt cagccggcgc ctgagcgcac gcgcgccgat tacgcgctgc
     5461 tgatgcgcta ccggcagaac aaggacgcgg ccgcgcgcga cgagctggtc gagcgcttcc
     5521 tgccgctcgc gcgccagctc gcacgccgct acgcgcgtcc gaacgagccg atcgacgacc
     5581 tcttccaggt cgcgagcatg ggcctgctga aggcgatcga gcgcttcgat cccgcccgcg
     5641 gcaacgcctt ctcgaccttc gccgtgccca cgatcgtggg cgagctcaag cgctacttcc
     5701 gcgacaccgg ctgggccgtg cacgtgccga ggccgatcca ggagcggatc gggcaggtca
     5761 accgcgccgt gaacgatctc tcgcgcgagc tcggccgctc ccccacgccg acggagctgt
     5821 cggccaagct cgggcactcg accgaggaga tcctcgaggc gctcgaggcc gcgaaggcct
     5881 tcgacgccgt ctccctggac atgccgcgcg gcaacggcga cgacagctcg gcctacgccg
     5941 acacggtcgg cgcgcacgac gagcgcttcg agatggtcga gtacaacgcg gtgatcgagc
     6001 cgaccatggc cgcgctgccc gaccgcgacc ggctgatcct gcggctgcgg ttcgagcgcg
     6061 acctgacgca gtcggagatc gccgaccggc tcggcatctc gcagatgcac gtctcgcgca
     6121 tcatccgccg ctcgctcgac cggctccgca cggtcgccga agccgcctag cccgctggtc
     6181 ctccgccgcg acgccgacgg cgacggggcg ctcgccatcg gccagccctc gcacgcgtgg
     6241 gtgtcgggtc agctcgcgcg cgcttggggc gacgagctcg acccgcatga ggaggtctgc
     6301 ctcgcggccg agcagcacga catcggcatg gccgagtggg acctgcggcc cacgctcaac
     6361 cgcgacacgg ggctgccgca ctcgttcatg gagatgccgc tcgacacgca cctcgagctg
     6421 tggtcgcgtg cccccgcgaa gctgctgcgt cagagcgcct acgcggcgct gctggtgtcg
     6481 atgcacggca cggcgctcta cgagatgcgc gacctcgacc gcatggatgc cggcgacgcg
     6541 gacaaggtgc gccgtttcct cgccgagagc cgcacggagc aggacgccct gctcgcggag
     6601 ctgggccagg agcgcgccgg cgtgctcgtg aaccagcggc tcgtctggac gtgggacttc
     6661 atgtcgctcg cgctctgcct cgactgggcg ccgacggtcg cggagcgggt cccactcggc
     6721 ggtgcgctcg tcgacatgcg gctcgagccc gtgggcccct cggccctcgc catcgacccg
     6781 tggccgttcc gggccccgtc ggtcaccgtc cgctgcgagg gccgcgcgct cggcggcggc
     6841 taccgcgacg agcgggagct gcacgccgcg ctggcggccg cgccgccggt accggtccag
     6901 tggaggctcg ttccggctta agctgcatcc atgcggggtg cgctgatgct ggtggtggcg
     6961 gtgctcgtag ccgccgtggc cggcgccccg gccgccgccg cggagttcac gcccgacggc
     7021 gaggcggcca actacgcgaa gatccgcgag cgcgctcagt acgagcacgg aacccccgag
     7081 taccgcgcgc tgatgcagga gcgcgggatc gagtcggagc tcgagctcaa cgacatccgg
     7141 ctgaacgatc ccgagcgcga catgagcggc aacctgtgcc gcagccacta cgacggctgc
     7201 gccggcgaca tacggctctg gcccaagcac tgggcggccg aggggcacgg gatcacgcgg
     7261 ccggttctct tcaccgcccg caacggggcg gtgctctccg ggcacgtgtg ggccacgcgc
     7321 tcggggccgg cgctgcggcc ggcggtcgtg atcacgtccg gctcgatcca ggcgccggag
     7381 gagctctacg tgttcgcggc cacgacgctg gccaaggcgg gctacgtggt gctcacctac
     7441 gacgtgcagg gccagggtcg ctcggacacc tacggcgagg gggtcgaccg cgacgagggc
     7501 tttccctcgc aggcggggca gccgttctac gacggcaccg aggacgcgct cgacttcatg
     7561 ctctcgaccc ccgcgagccc gtaccggccg cgcaggagct gctcgaccgg cacctcgcac
     7621 gaggagaagc agctgcgacg cgtgcgggcg ggcctcaacg ccgcctacaa cccgctgtcc
     7681 gagctggtgg acccgcggcg ggtcggcgtg gccgggcact cgctgggcgc ggcggcggtc
     7741 tcgtacgtgg ggcagctcga cgcgcgcgtg aaggcgatcg tggcgtggga caacctgcgc
     7801 gactcgagcg cggggatcgc ggcctccgcg tgcccctccg gcgcgtcccc gcggccggag
     7861 cggatcgcgc tgacgaagcc ggcgctcggc atgtcgaacg actacagcct gacccccacg
     7921 ccatacagcg ccgcgccgga cgagcaggcc aagaacgcgg cgtcgaacgg gtacagcgcc
     7981 gccggcgtcg acagcgccca gctcaacatc cgcggcggca cgcacttcga gtacgcgtac
     8041 atcccgaacc ctggtttcgg cgcgacgctg cgcgggatgg acatggccgc ctggtacatg
     8101 caggcgtggt tcgaccgcta cgtgaagggc ggcgtggacg aggacgcgga gcggcggctg
     8161 cggaccacgc gctggcgcgc ggacgcgcgc ggcgccgagg tggatcccgc gggtgacggc
     8221 aacctgttct cgcactacct gaagtcccgg ctcgcgatcg ggggcttccg ctgcgaggac
     8281 atgcgcacgg gatgcgccgg gctgagcgcc gacgacgggc gcccgccgaa ctactcgtac
     8341 tacgacgtgg cgaagacggc cgacgacggc gcggtaacgc cggccgccgc ggacttcgat
     8401 gggaccgcgg aggtgacggt cacgaaggcc gccgagacga gcggcgccga cgggtcgttc
     8461 cccgtgcgcg tgcgcgcggt cgctcgcagg gaggaggccg accacttcga gctccaggtg
     8521 tcgcgctact cgccgcgccg cgcgggggcc taccggacga tcgcagggca gctcggcgcc
     8581 gcgccgcacg tgttccgcgg ggcactcggc gcgacctacc gcttccgtgc ccgcgcggtg
     8641 tcgcgcgggg gcgcggccgg gccgtggtcg cgcgcggcga tcacgatggt gccgttcgac
     8701 gctgcgcccg ggcgggtgaa gttcggccgc ggctggtcga gcgtgagcga cgcgcgcgcc
     8761 tggaacggct cgttccggcg caccacgcgc gcgggggcga cgatggcgtt ccgctggcgc
     8821 gggtcaatcc tccacctggt cggccgccgc tcgccggccg gcggccgcgc ctggctctac
     8881 gtggacggcc ggcggcggtc gatcagcttc cgctcccgca aggtccgcaa tcgcacgctg
     8941 atctgggccg aaaaggtcag cggccgcggc acgcacacga tgcggatcgt gacgcgcggc
     9001 ggtcgcgccg aggtcgacgg gatcggcgta gcgccctagc gccaaaccgg cctggcaggt
     9061 ttcgcacgag gtcgtcctgg aaaaaagagc taaagctctt cggagctcgt gccgatgatc
     9121 ggtacgaaga cttcctttag ggcgaaggac gttctcttcg tccgaggcac gacgccgctg
     9181 gaggttgggc tagtacctat gctgagaagg ggtccgcgga gcgggcccct ctctctttct
     9241 ggggtcggtc gtaggcttca ggcgtgcccg accgtgacga cctcgccgag gccctcgcgc
     9301 ggcgcgcggc cacacgcgac gaggcgcggc cggaggctgt ggagcgccgg cacgcggggg
     9361 gcgggcgaac ggcccgggag aacatcgccg acctcgtcga ccccggcagc ttcgtcgagt
     9421 acggggcgct cgccatcgcg gcgcagcgcg cgcggcgcga gctccaggag ctgatcgagc
     9481 gcacgccggc cgacgggctc gtggcgggca ccgcccgtgt gaacgaggac ctgttcgggg
     9541 agcgcagcgc gtgcgccgtg ctctcgtacg actacacggt gctggccggc acgcagggcg
     9601 tgctcggcca ccgcaagaag gaccgcctgt tcgagctgat cgagcgcatg cggctgccga
     9661 ccgtcttcta tgccgagggc ggcggcggcc ggcccggcga caccgactac ccggtcgtct
     9721 cggcgctcga cacgcgcgcc ttcgcgcttt gggcgcggct ctccggcctg gtgccgcgca
     9781 tcgccgtggt cgcggggcgc tgcttcgccg gcaacgccgt gatcgccggc tgctcggacc
     9841 tgatcgtcgc gaccgagaac gcgtcgatcg gcatgggcgg cccggcgatg atcgagggcg
     9901 gcgggctcgg gcgggtcgat cccgacgacg tcgggccggt cgacatgcag gctggcaacg
     9961 gggtgatcga cgtggtcgcc gccgacgagg ccgaggccac cgcggtgacc aagcgcctgc
    10021 tcgcctactt ccagggtccg acgccgcccg gcgaggagcc cgaccagacg gtcctgcgcg
    10081 acatcgtccc gcagcggcgc cggcgcgcgt accaggtgca gccggtcatc gagacgctgg
    10141 ccgacgcggg ctcggtcacg ttcctgcgcg agcggtgggc ccgcgagatg gtcaccgcgc
    10201 tggttcgcat cgaggggcgt ccggtcggga tcgtggccaa caacacgatg cacatggcgg
    10261 gcgcggtgac gagcgaggga gccgacaagg cggcgcgctt catgcagctc tgcgacgcgt
    10321 tcgggctgcc gctcgtctcg ctcgtggaca cgccgggcat gatggtcggg cccaccgcgg
    10381 aggccaccgg cctcgtgcgc cactgcagcc gcctgctcgt cacaggcgcc tcgctccgcg
    10441 tgccgctggt cgcggtggtg ctgcgccgcg gctacgggct gggtgcgcag gcgatggtcg
    10501 cggggagcct gcacgaaccg ctgctcacgg tcgcgtggcc cacggccgag ctcggcccga
    10561 tgggactcga gggagcggtg cggctcggct tccggcgcga gctcgagacg atcgccgacg
    10621 aggccgagcg caaccagcgg gtgcgcgagc tgaccgagct ggccgagcag aacgcgaagg
    10681 ccgtgaacgc ggcgacgctg ttcgagctcg acgacgtgat cgacccggcg gagacgcgcc
    10741 ggctgatcgc ctccacgttc gcggcggcgg cgccggccgc ggcggcggag cccccacgcg
    10801 ggttcgtgga cacctggtag tcaggtcagg gccgcgttga ccagcacgcc gatgctggcg
    10861 acgggcgccg ccaaagcaag tcggccggcg aagcgcgcta tgcgccgccc cacggtcacc
    10921 tcggcgagct cgaccgcggg gaagagcagc gcgtaggtcc gcgggccgat gggcaggtcg
    10981 tcgcgctgtg ccacagcgag caggccgtac agcaccggga gaggagcgaa cacgacgatc
    11041 agcagggacc acggcgtgtc gaagaagtgc gtgccagcga gcgcatccga agcggcgacg
    11101 aacacggctg tgatcccgta ggtgagcgcg aggtacggcc acgcgaagcg gccgatgcga
    11161 cgctcgacgc gctcgtgcag cgtcctgtcc cgcaccgccg ggtccgtgcc ggtgatcggc
    11221 tccgggttca acgggatgcg gccccgagcc agggtccggc ggaacccggc gcgacccacg
    11281 gagggccgga cgtcgttcag taccttgatc gcgcgcccgt cgctcgccgt tgagccgacc
    11341 tcgagcatca cgcccgggat gtccggaagc gtgattgcgg tcgaccgccc gcgcgcgatc
    11401 cgcaggtcca ggtcggtcgc cgtgccgcgc acggccaccc ggatcgcatc gaagcgcagg
    11461 gccgcccggg gcgtggccca ttccttcagg tcggccgggc tgcggaacag ctcgacgtcg
    11521 tcgcgcacgg cgatctccac gaagcagccg gggaccgcgc cgtccgcgcg cgtgatcgcg
    11581 agcaccgcgg cgcgcagggt gcgcgcgagg agcatcaggt cgccgcgcca ggcgctcgcc
    11641 acggcccacg cgtagctgcc ggagtcgcgc agggcggccg gcgcggcggg atcgacgatc
    11701 agcccgccga ccacgggttc cgagccaggc tcgggtgcta cggcggaggc gtccatggcg
    11761 agcggcgccg atcgtaggcg cgagcgcgga cgcgcccggc tcgagccgac gccgagcgtc
    11821 agcgcaccgt cacgcgggcg gtcgcgcgac cgcagcccgg cttggcgacc cggaccgtga
    11881 cggcggcggg cttcgacagc ttcttgcgca gcacggcgcg accggcggag tcggtggtgg
    11941 cccgcgcgcg gccgagccga acgctcgccc ccgcggcgca gccgggcgtc gccgagctga
    12001 cgcggacccg gagcgtcgcg cgcccgcccg ccgcgacggt gcgcggcgag acgcgcagct
    12061 tcagcctggg tgccgccggc cgctcgcagc tgagcgagtc gaagacggtg gtgctcccgc
    12121 cgtcgtcgaa gctgaacctg cccgcggcgc gccagaggcc gtcggccggg cagtccgggg
    12181 gcgtggtgac gtacacgcgc ccgccctcca cgcgcgccgg ggcggtcagc tccactcgct
    12241 tgatcgacgt gcgcccgtcc ggcggccctc ccggcagcgc gggtgggtgc gccctcagcg
    12301 tcgatccctc gatgccaatc cggtcgaacc cggcggcctc gtcggtcccg cggaccacga
    12361 cgatctcgat gatctcgccg gcgccgttga agaagacccc ctcgccgtcg aacgggtcga
    12421 tcggggtgcc ggtgtgcgcg acgagccgcc cctcaccgat cttcgtctcg ggcgggcagg
    12481 cgttgcgccc gcgcgcgtgg atctcggcgt ccgtggcggt gcactgaggc agcgccgcgt
    12541 tgtcgatccg ggtgccggat ggcagctcga acgccgcgct cgcgagcggc ggcggcttcg
    12601 cggacgggtc ggaagggttc ttgtactcga ccgccagcgt gaggccggtg ctcgccccgg
    12661 ggcgtgccgt ggtgaagccg acctcgagct cggcccgctc gttcgccgag accggcgccg
    12721 cgactgcgag cgcggcgatg gcggcggcga ccgcgcacct cgctagcttg cgccctgagt
    12781 gctccatccg cccgtcctct cgctcgcgtg gctccattcc gtacactgta cgggatggcg
    12841 tacggaagag cgccggtccg ccgcatgacc cctcgtacga gctccgggct cacgcgcgcc
    12901 gcggtcgcac acgcagcgct ccagcgactc gacgcggagg ggctcgacgc gctcagcatg
    12961 cgccgcctgg ccggcgagct gggtgtgggg acgatgacgc tctacggcta cttccgcagc
    13021 aaggaggagc tgctcgacgc cgtggtcgac gccggcttct ccgagttcga gatgccgccg
    13081 gcaggcgacg acttccgcac caccctgcgc gacttcgcgc tcgccgcgcg cgcggtgctc
    13141 gttcgccacc cggcgctagt cgagatccgc gacgccggcc cgatcctgcg tccgcgcggc
    13201 ttccaggtca cggagctggg cgtgcgcatc ctcagcgagg ccgggctggg cgccgaggac
    13261 gcggcgcgca gcttccgcct gctgttcgac tacgtcttcg gctacgcgat cgtcaaccgc
    13321 cgcccgccct cgccggagct gcgccgcgag gcactcgcgt cgatcgtggc cctgccaccc
    13381 gaggagttcc cgaccgtgac ggcggtggcc gagcagatgg ccgaggcggt cggcgcggag
    13441 gggcagttcg agtacgggct cgaacgcatc ctcgacggga tcgaggcgcg gctggcggct
    13501 agtcgcgccg ggagaagggc gagaccgggc gctcctggag ctcgccgtcg aggtacaggt
    13561 ccacgacctc gttaaggaag cagagccggt cgcggatccg ctcggcgtcg cgcagcggct
    13621 cggggtagta ccagacgacg tcgcgctcct cggtctcgcc cacgcggacc gagtggtaca
    13681 acgcgtagcc cttgtaggca cagccggtgc gggtgtcgct ggggacgagc gcgtcgtcac
    13741 gcacgtcctc gcgcggcagg taccagcgcg gcggcaggcc agtctcgaac agcgcgcggg
    13801 cgcgggtgct ctcggccacg gtctcgccgc cgatctcgac gcgtacgtgg cgcgaggtgt
    13861 cgagcacgtc ggtgcggtgg tagggatcgc gcatgtgcac catcagcggc tcgtcctcct
    13921 cgagccaccc gtccacacga tcccagtaga acgcgacatg gccagcgagc ccggccgcgc
    13981 cctccagcgg ggccgggtag ctccaggcgg cgtccttggc gacggagtcg cccacgcgca
    14041 gggtgtggtg gcgggcgtcg cccttccacg gacagtgcgt ggcgtggtcg ctcggctcga
    14101 agaggtccgt gcggacctcg tcctccggga agtacaggat cgggagcagc ccgtgctcgt
    14161 ggagcagctt ggcgcggcag ctgtccacga cggtctcgcc cgcgaagcgc gcgcggatcc
    14221 ggcgcgggaa gtcctcgaag tagatgagcc ccttcagcgg cgggagctcg cgattgaacg
    14281 tgcccgcggg ggtgtggccg aaggggccgc gtccgacggt gagtgccatg ccgtcaggtt
    14341 acgcgggagg agtggccggg gcggcgcccg cgcgaggcgg
//
LOCUS       DASVPL010000184        13595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_138145, whole genome shotgun sequence.
ACCESSION   DASVPL010000184 DASVPL010000000
VERSION     DASVPL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13595
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138145"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="VF517_09450"
     CDS             complement(<1..1278)
                     /locus_tag="VF517_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053206.1"
                     /translation="MAPGRRMLLDAAAAALVGLLVFAAFGHSFLNYDTFYSLVWGDDL
                     VSGRGPQYEVPVAPTPHPLAVAVGALVSPLGDGAEDVLLALVILSIGAIAVGLFRLGR
                     ELFAWPVGLLAAAIFLTRVPPLNFGIRGYVDLPTIALVVWAAVLEVRRPRRGAAVLVL
                     LGLAGLLRPEAWLLAGAYWLWLVWPARAGAAAPAEGAGRAAPAPLDRGRALRLAALAA
                     AAPLLWALSDLLVTGDPLHSLHGTSELAADLGRPRGLDDVPRVMPRRLGEIMRLPELL
                     AATAGFGLALAWLRERAAVPAAILVLNGVGFLVLAVGGLSLLGRYLFLAGAMLSLFAA
                     VAVFGSMALAPDDRRRRAWMAGGIVLLVTMAALAPQQAGRLGDLRDDIADRDRVAADL
                     HDLVENPAAREALDRCGTLFVPNHRPVPNLAYWT"
     gene            1333..3258
                     /gene="dnaX"
                     /locus_tag="VF517_09455"
     CDS             1333..3258
                     /gene="dnaX"
                     /locus_tag="VF517_09455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX8053207.1"
                     /translation="MLSLYRRHRPRTFDEVVGQEHIVRTLRNAIALEKVHHAYLFVGS
                     RGTGKTSMAKLLACALNAEGGPRADFPPDDPAARAIMTGQSLDVVEMDAASNNSVDDI
                     RELRENVALAPMSGSRRVYILDEAHMLSTSAWNAFLKTLEEPPPHVVFVLATTEAHKV
                     PATIVDRCHRFDFHRPTLEQIADVLGRVSEQEGIEVPDGALPMIARAATGSFRDALGT
                     LEQLVTYGGKEIGLDDVLEILGVADAELVFECADALSTHDPKAALLAVERLSRSGRDV
                     EQFTGDLAGHLRHLYVVQTLGEVPTSFAVTAEQTDRVAAQAGQIPGGELLRAIDLLAT
                     ALRAAKDGSDPRIQLELALLKAVQPQADLSLQALLFRIEQLERRLEEAGAGAPAAGQP
                     AAAPVTAPPVAPPRVEIEVGAEGGTGAETAADAEAGTRAETAAGAEAGARAESEAGAE
                     PQAAAEPSRAAPAEPSPAAPPPPAPEPRVAAASHAGAAAAAAVAPEPEPDPEPVAEPE
                     PVPVAAAGDATGADLDRLKTLWPAVVDQVRSENAMVGACLDAARPLKVQGDRLTICFP
                     VMGGFSKKTVEKNRELVQSSLRALTGSAFALDYEMNDDAPESPVAVGLSDEELIERLK
                     RDYGATEIFDDEPEQEA"
     gene            3281..3601
                     /locus_tag="VF517_09460"
     CDS             3281..3601
                     /locus_tag="VF517_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX8053208.1"
                     /translation="MNKMLKQVQEMQAEMVKAQEEAEKQEVEASAGGGMVTVKVNGAL
                     SVLEVKIDPDAVDPDDVEMLQDMIQAATNEALRSAQEMVASKMGQVTGGLESALGGLG
                     LPGM"
     gene            3682..5091
                     /locus_tag="VF517_09465"
     CDS             3682..5091
                     /locus_tag="VF517_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8053209.1"
                     /translation="MKDLIASLARARDIAPGVRTAFRLGSITAALTVSLVLFGWSTAS
                     AAVAIALGIGLVVGWARGSEPQTSMAAELEETRRRLDSLALRDPLTGVLNHRAFQDQL
                     ETELRRARRESWPVAVVAVDIDDFREVNDVKGHAHGDALLVTVSEALASNLRPGDLCG
                     RVGGDEFMLALSGCDADAAREVLARLRSGIDSRAMAASVGVISVSAGIAEFPGHSVGR
                     AELMRFADSAMQRARGASPGATAVHSVDDTPLSLDDEASQAQRGALFNTVHALARAVD
                     ARDGFTHMHSQRVAFYAATLANAMKLGDERVEEVRMAGLLHDVGKIGIRDGILLKPTG
                     LTKEEFTVMRRHSELGRAIIAGAGLVEIAGWVGSLHERVDGAGYPEALSGEGIPLESR
                     ILHVADALEAMTTPRAYRDPMTPEDAVCELERNAGSQFDEGAVSVMARLVRERAIEVG
                     EQPRLGHPLAGGASAFAAD"
     gene            5122..5721
                     /gene="recR"
                     /locus_tag="VF517_09470"
     CDS             5122..5721
                     /gene="recR"
                     /locus_tag="VF517_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX8053210.1"
                     /translation="MFSPPVNRLITELARLPGIGQRTAQRLAFHILRTPTDEALPLAA
                     AIREVKETVGFCEICFNLAVGPKCRICEDERRESDVICVVEEPSDVIPIERTHEFRGR
                     YHVLGGALSPIDGIDPEDLRLAELVTRVEQGGTREVVIATNSTTTGEATALYIADTLR
                     ERAPDVSVTRLASGLPVGADLEYADEVTLGKAFQGRRAL"
     gene            complement(6049..7398)
                     /locus_tag="VF517_09475"
     CDS             complement(6049..7398)
                     /locus_tag="VF517_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8053211.1"
                     /translation="MRPAIVRHLRNLPPLRAGLALALGVVLVVLLASGGDDGDDRKKP
                     APAPAPRAAAAPAGSFLARIVPSPATGLPGARVPGVIRRLVARLPAERKAAQLMLVGF
                     QGRDPSATFFRTLRRSDFGGVVFERRNYRDSVQLEAMTASVANATARKNHEPPLMLAR
                     QAGGEQSAFPDFPPAGEPGELAGLPDAVDQFADTTRALRAVGLNGVIGPAVDVAPNEA
                     GALGTRAFSDQPDEVAAFAKAGVAAFRKAELLSGVEHFPGIGGATQHTDEGPAQVGLS
                     LDELRERDLVPFRAAIAAGAPAVVVGHASYATDDFVLPASLSRTLATDVLRGGLGFRG
                     IAISDDLAAGAIVTTQPSLPKAAVDAIVAGVDMVWLSGPPGVQAKVYRTILAAIRKGT
                     ISRGRLDTAVTRIITVKRELGLRVRRRTPPPQYPNAANPGSGQVEPVAPGAAPPPAP"
     gene            7425..8777
                     /locus_tag="VF517_09480"
     CDS             7425..8777
                     /locus_tag="VF517_09480"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX8053212.1"
                     /translation="MAGPATDRAGEPASDPRRTVVMKFGGTSVADAERIRRAAGRIVR
                     AKERGDDVVAVLSARGKTTDELVQMAHEVSARPHPREMDMLLSTGERISCALASMVIN
                     DLGYDAVSLTGSQAGIVTDASHTKARIIDVRTDRIEGLLGEGRVVLVAGFQGTSAALR
                     GDGGGYEVHGDVTTLGRGGSDTTAVALAAALNAHVCEIYTDVAGVFSADPRIVPEARK
                     LNYVSFEEMLEMSASGAGVLQLRSVEYARNHGVRIHCRSSFDDGPGTLVLMEEETMER
                     PMVTAVTHSTSEARVTLMSIPDQPGIAGRVATALADANVNIDMIIQNEPQSEGHRADM
                     SFTVPRDELELAREALEPLVGDMGIGQIATDSAIGKVSLVGAGMKSHPGVAAKTFSVL
                     GDSGVNIEMISTSPIKISCVVREDDVAPAVRALHRAFDLGEDAIRREDPRGDHRPVVS
                     "
     gene            8777..9808
                     /locus_tag="VF517_09485"
     CDS             8777..9808
                     /locus_tag="VF517_09485"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX8053213.1"
                     /translation="MRVALVGATGAVGSIMLEVLQERRFPATEVVPFASERSVGRKLA
                     FGDGTIEVQALTEESIAGFDIAIFSAGGSTSGEWAPRFAAAGAVVVDNSSRWRMQDDV
                     PLVVSEVNPDDLDNHRGIIANPNCSTMQMVVALKPIDVVAGIERLVITTMQSVSGTGQ
                     KAVEALHDETEAVLAAKELAKPEVYAHQIAFNVLPHAGTFPEGSDYTDEELKLVNETR
                     KILGKPDIAVSATCTRVPVYTGHSESVNVQTREPLSADDCRALLTRSPGIVVLDDPAH
                     GVYPLAIDAAGRDEVLVGRIRQDPSHPRALNMFVVADNLRKGAATNAVQLAELLVERG
                     LVRARAQAA"
     gene            9917..11812
                     /locus_tag="VF517_09490"
     CDS             9917..11812
                     /locus_tag="VF517_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053214.1"
                     /translation="MRGHTGGWGGPRRALLATVAALAILAPSAAAQSTARPPTPEPNT
                     AVDIAPRPCAVEGRDRYEREFYEREGWPGPDHERYPGACQRLRFSYGPIAVKPGQNDV
                     LIEPVKIEKPLREGYITRFKPDLVRADGVVPPIEQVHLHHATWLAEPDYGSGPFFAAG
                     EEKTVAPFPKGYGMPVKPSDTWLLLYMVHSAIQQPMEVYITYDIDFVPKDKGEALGLK
                     KAYPLWFDVRPSGYPVFNVQRDFGGADGICTWPTEKCAAHDPFGKEIVGQGQPGNGKG
                     TDWSFPKPGETLGRVENFNGGTIVGIGGHLHPGGLQNTIDLVRPGNTVRVKKKIKVKT
                     KSKKGRKRKTKTKTVYVTEKREQARIYTGDAYYWDRKDRMKAGGPPTSWDFSMGVGGL
                     PDWGVHVKPGDIIRSNATYDTTHQSTYENMGIVVSMFVPNDENGKPQAPGVDPFVTPH
                     DPTPTCDSGGVRAKPPRLCDRGIPTHGHLTENDNFGGPQGEWKAERGVETDRVGIADF
                     LYAPGDLSMISMTGVPTVKLGSDLKFTNYDGAVIYHTVTSCKFPCLGQTGTSFPISDG
                     QTSKGRNLDFDSSELGFGTPTIGPTKQTLNWSLPVSKEAGFEPGEIVTYFCRVHPGMR
                     GAFEVQQ"
     gene            11809..>13595
                     /locus_tag="VF517_09495"
     CDS             11809..>13595
                     /locus_tag="VF517_09495"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8053215.1"
                     /translation="MSNFGEDIPFLARNKPYRHGWADLSEAPPVDVLPCSNDEYFPPP
                     PTREQIGIMQLQSREIERYRRRFNMSRRAFVRTAAATAIGFWAIDAVRQGEYGNYGWA
                     ANTTTDACDLEWDGKKGMESLTNLPGEFIFDVQSHHVDAEGQWRVTNPGFHAFFAALW
                     PQSSWATGEPGGVREDGSIRGGGEGGEVDPIQNLSRFHYLKELYLDSATTATVLSIVP
                     TSPDTNNPLPLAEGYKTARLVNDLSKARRCVLHAFVMPNRGSSGTTNPGGSPIKPLYL
                     EEEMDLMMQRADQYGDLLRGWKTYCAWGDVPNASGWTFDTPVGERFLENVLAVSKKYP
                     QIPPVVATHKGFPLPAFDQRAATPRDIGPAAKAFPGVRFLVYHSGYDTGDRQTAYRGD
                     DEARSDTHTVDGFIKSLRENQWDASRFVKKGKKFGNVPNVYAELGSVWRGVMNDPDQA
                     AHLLGKLINHVGPKRVVWGTDSLWYGSPQPEIVALRRFEFTQKGKEFYGLPYGLNGDV
                     EDPRKKAPRPSRTIRNAILGYNAARAYSFDPVKIAEEIACDEVQRLKEEGYLRGGGTE
                     TESAPLASHVAPGARTKAEVIHNVYHSGE"
BASE COUNT         2061 a         5051 c         4783 g         1700 t
ORIGIN      
        1 ggtccagtag gcgaggttcg ggaccgggcg gtggttcggg acgaacagcg tgccgcagcg
       61 atcgagggcc tcccgcgcgg cgggattctc gacgaggtcg tgcaggtcgg cggcgacgcg
      121 gtcgcggtcg gcgatgtcgt cgcgcaggtc gccgagccgg cccgcctgct gtggcgcgag
      181 cgcggccatc gtgaccagca gcacgatccc gccggccatc cacgcgcggc ggcgccggtc
      241 atcgggcgcg agcgccatcg acccgaatac cgccaccgcg gcgaacagcg acagcatcgc
      301 gcccgcgagg aacaggtagc gcccgagaag cgacaggccg ccgaccgcga gcaccagaaa
      361 tcccacgccg ttcaggacga ggatcgccgc gggcacggcg gcgcgctccc gcagccacgc
      421 gagcgcgagg ccgaagccgg cggtggccgc gagcagctcc ggcagccgca tgatctcgcc
      481 gagccgccgc ggcatcacgc gcggcacgtc gtcgaggccg cgcggtcgcc cgaggtcggc
      541 ggccagctcg ctcgtcccgt gcagggagtg gagcgggtcg ccggtgacga ggaggtcgct
      601 gagcgcccac aggagggggg cggcggccgc gagcgcggcg aggcgcagcg cgcggccgcg
      661 gtcgagcgga gccggcgcgg ctcggccggc gccctccgcc ggggccgcag cgcccgcccg
      721 cgccggccac acgagccaca gccagtaggc gcccgcgagc agccacgcct ccggccgcag
      781 cagccccgcc aggccgagca gcaccagcac cgccgcgccg cgccgcggcc ggcgcacctc
      841 gagcaccgcg gcccacacga ccagcgcaat cgtcggcagg tccacgtaac cgcgtatccc
      901 gaagttcagc ggcggcacgc gcgtgaggaa gatcgcggcc gcgagcagcc cgaccggcca
      961 cgcaaacagc tcgcgcccaa gcctgaacag cccgaccgcg atggccccga tcgaaaggat
     1021 cacgagcgcg agcagcacgt cctcggcccc gtcgcccagc ggcgagacca gcgcccccac
     1081 cgcgacggcc agcgggtgcg gggtcggggc caccggcacc tcgtactgcg ggccgcgacc
     1141 gctcaccagg tcgtcccccc agacgagcga gtagaaggtg tcgtagttga ggaacgagtg
     1201 cccgaaggcc gcgaacacga gcaggccgac gagggccgcc gccgcggcgt ccagcagcat
     1261 ccgccggccg ggagccatgg cgcgggacgc tatccgcccg ccctccggac ccgtggctaa
     1321 agtccccggt ccgtgctctc gctgtaccgc cgccaccgcc cgaggacctt cgacgaggtg
     1381 gtcggccagg agcacatcgt ccgcacgctg cgcaacgcga tcgccctcga gaaggtccac
     1441 cacgcgtacc tcttcgttgg ctcgcgcggg accggcaaga ccagcatggc caagctgctc
     1501 gcctgcgcgc tgaacgccga gggcggcccg cgggccgact tcccgccgga cgatcccgcc
     1561 gcgcgcgcga tcatgaccgg ccagtcgctc gacgtggtcg agatggacgc cgcctcgaac
     1621 aactcggtcg acgacatccg cgagctgcgc gagaacgtcg cgctcgcccc gatgtccggg
     1681 tcgcgccgcg tctacatcct cgacgaggcg cacatgctct ccacctcggc gtggaacgcg
     1741 ttcctgaaga cgctcgagga gccgcccccg catgtggtct tcgtgctcgc cacgacggag
     1801 gcgcacaagg tgccggcgac gatcgtggac cgctgccacc gcttcgactt ccaccggccc
     1861 acgctcgagc agatcgccga cgtgctcggc cgcgtgtccg agcaggaggg gatcgaggtg
     1921 cccgacggcg cgctgccgat gatcgcccgc gcggccaccg gcagcttccg cgacgcgctc
     1981 ggcacgctcg agcagctcgt cacctacggc ggcaaggaga tcgggctcga cgacgtgctg
     2041 gagatcctcg gcgtggccga cgccgagctg gtcttcgagt gcgccgacgc gctctccacg
     2101 cacgacccga aggcggcgct gctggccgtg gagcggctgt cgcggtcggg ccgcgacgtc
     2161 gagcaattca ccggcgatct cgccggtcac ctgcgccacc tgtacgtggt gcagacgctc
     2221 ggcgaggtcc cgacctcgtt cgccgtcacc gccgagcaga ccgaccgggt ggccgcgcag
     2281 gccggccaga tcccgggcgg cgagctgctc cgcgcgatcg acctgctcgc caccgcgctg
     2341 cgtgccgcga aggacggctc ggatccgcgc atccagctcg agctcgcgct gctcaaggcg
     2401 gtccagccgc aggcggacct ctcgctccag gcgctgctgt tccggatcga gcagctcgag
     2461 cgccggctgg aggaggcggg agccggggcg ccggcggcgg gccagcccgc cgccgcaccg
     2521 gtgacggcgc cacccgtggc gcctccgcgt gtggagatcg aggtcggcgc ggagggcggc
     2581 acgggtgctg agaccgcggc cgatgcggag gccgggacgc gcgcggagac cgcggccgga
     2641 gcagaggccg gcgcgcgcgc cgaatccgag gccggcgcgg agcctcaggc cgccgccgag
     2701 ccgagccgcg cggcccctgc cgagccgagc cccgcggccc cgccgccacc ggccccggag
     2761 ccccgcgtcg ccgccgcctc ccacgcgggc gccgccgccg cggccgccgt ggctcccgag
     2821 cccgagcccg accccgagcc ggtcgccgag cccgagccgg tccccgtcgc cgccgcaggc
     2881 gacgccaccg gcgccgacct cgaccgcctc aagacgctct ggcccgcggt cgtcgaccag
     2941 gtccgctccg agaacgcgat ggtcggcgcg tgcctcgacg ccgcccgccc gctgaaggtc
     3001 cagggcgacc gcctcacgat ctgctttccg gtcatgggcg ggttctccaa gaagaccgtg
     3061 gagaagaacc gcgagctcgt gcagtcctcc ctgcgagccc tgaccggcag cgccttcgcg
     3121 ctcgactacg agatgaacga cgacgctccc gagagcccgg tcgcggtcgg gctcagcgac
     3181 gaggagctga tcgagcgcct gaagcgcgat tatggggcga ccgagatctt cgacgacgaa
     3241 cccgagcagg aggcctgacc aggtgccgca gccgcccaac atgaacaaga tgctcaagca
     3301 ggtgcaggag atgcaggccg agatggtcaa ggcgcaggag gaggccgaga agcaggaggt
     3361 cgaggccagc gcgggcggcg ggatggtgac cgtcaaggtc aacggcgcgc tctcggtgct
     3421 cgaggtcaag atcgacccgg acgcggtcga cccggacgac gtcgagatgc tccaggacat
     3481 gatccaggcg gcgaccaacg aggcgttgcg ctcggcacag gagatggtcg cgagcaagat
     3541 gggccaggtc accggcgggc tcgagagcgc gctcggcgga ctcgggctgc ccgggatgta
     3601 gagccgggcg ccgcgcgcgc ccgccgcttc cgccgtccgg aagcgctcaa ggaggcctcc
     3661 ccggctgccg atgcagcctc gatgaaggac ctcatcgcat cgctcgcgcg tgcgcgagac
     3721 atcgcgcccg gcgtgcggac cgccttccgc ctcggctcga tcaccgcggc actgaccgtg
     3781 tcgctcgtcc tcttcggctg gtccacggcc tcggccgccg tggcgatcgc cctcggcatc
     3841 ggcctggtgg tgggctgggc gcgcggcagc gagccgcaga cctcgatggc cgccgagctc
     3901 gaggagaccc gccgccgcct cgactccctc gccctgcgcg acccgctcac gggcgtcctc
     3961 aaccaccgcg ccttccagga ccagctcgag accgagctgc gccgcgcccg ccgcgagagc
     4021 tggccggtcg cggtcgtggc cgtcgacatc gacgacttcc gcgaggtcaa cgacgttaag
     4081 ggccacgcac acggcgacgc cctcctcgtg accgtctccg aggcgctcgc ctcgaacctt
     4141 cgccccggcg acctctgcgg ccgcgtgggc ggcgacgagt tcatgctcgc cctgagcggc
     4201 tgcgacgccg acgccgcccg cgaggtgctc gcccggctgc gctccggcat cgactcgcgt
     4261 gccatggccg cctcggtcgg cgtgatctcc gtcagcgccg gcatcgccga gttcccgggc
     4321 cactcagtgg ggcgcgccga gctgatgcgc ttcgccgaca gcgccatgca gcgcgcccgc
     4381 ggcgcctcgc ccggcgccac cgccgtccac tcggtcgacg acaccccgct ctcgctcgac
     4441 gacgaggcgt cgcaggctca gcgcggcgcc ctcttcaaca cggtccacgc gctcgcccgc
     4501 gcggtcgacg cccgcgacgg cttcacccac atgcactccc agcgggtcgc cttctacgcg
     4561 gccacgctcg cgaacgccat gaagctcggc gacgagcgcg tcgaggaggt ccggatggcc
     4621 ggcctgctcc acgacgtcgg caagatcggc atccgcgacg ggatcctcct gaagccgacc
     4681 gggctgacca aggaagagtt cacggtgatg cgccgccact ccgagctcgg ccgggcgatc
     4741 atcgccggcg cgggtctcgt cgagattgcc ggctgggtcg ggtcgctgca tgagcgggtc
     4801 gacggcgccg gctaccccga ggccctgagc ggcgagggga tcccgctgga gagccggatc
     4861 ctgcacgtcg ccgacgcgct cgaggccatg accaccccgc gcgcgtaccg cgacccaatg
     4921 acccccgagg acgccgtctg cgagctcgag cgcaacgccg ggtcgcagtt cgacgagggc
     4981 gccgtcagcg tgatggcccg cctcgtgcgc gagcgggcga tcgaggtcgg cgagcagccg
     5041 cgcctcgggc atccgctggc cggcggcgcg tcggcattcg ccgccgactg agcgcccggc
     5101 ccgccggata acctcggcgc ggtgttctcg ccgcccgtca atcgcctcat caccgagctc
     5161 gcccggctgc ccgggatcgg ccagcgcacc gctcagcggc tcgccttcca catcctccgc
     5221 acccccaccg acgaggcgct tccgctcgcg gcggcgatcc gcgaggtgaa ggagaccgtc
     5281 ggcttctgcg agatctgctt caacctcgcc gtcggcccca aatgccggat ctgcgaggac
     5341 gagcggcgtg aatccgacgt gatctgcgtg gttgaggagc cgagcgacgt gatcccgatc
     5401 gagcgcaccc acgagttccg cggccgctac cacgtgctgg gcggcgcgct ctccccgatc
     5461 gacgggatcg atccggagga cctgcgcctc gcggagctcg tcacgcgcgt cgagcagggc
     5521 ggcacgcgcg aggtcgtgat cgccacgaac tcgaccacca ccggcgaggc caccgcgctc
     5581 tacatcgccg acaccctgcg cgagcgtgcg ccggacgtga gcgtcacccg gctcgccagc
     5641 gggctgcctg tgggcgcgga cctggagtac gccgacgagg tcaccctcgg caaggcgttc
     5701 cagggtcgtc gcgcgctgtg acgggggccg agccgcgctg gctccgcagg gccgcgcgcg
     5761 tcggcttcgc gatcctcgcc ctcgccacgg tcctgctccc ctggtaccgg ctggacgact
     5821 acacgccgaa cggctgggac gccacgtggt tcgcgcggat cgccctggtc gccgccgccg
     5881 tcgggctggt ggcgggcccg tcgcgggtcc gggtcgcggc ggccgcgatg gcggtgctcg
     5941 cggtggccta caggcttgcg gtcccgcccg acttcggctt cgccttcgac gggctcgagg
     6001 tgcccacgga gcggcgcatc ggggtggccg tcgccctcgc ggctgcgctc acggcgctgg
     6061 tggcggagct gctccgggcg ccacgggctc gacctgaccc gagcccgggt tcgccgcgtt
     6121 cgggtactgc ggcggaggcg tccgcctccg aacccgcagg cccagctccc gcttgacggt
     6181 gatgatccgg gtcaccgcgg tgtcgagccg cccgcgcgag atcgtgccct tgcggatcgc
     6241 ggccaggatc gtgcggtaga ccttcgcctg aacccccggc ggcccggaca gccagaccat
     6301 gtccaccccg gcgacgatgg cgtcgaccgc cgccttcggg agcgacggct gggtggtcac
     6361 gatcgcgccc gccgcgagat cgtccgagat cgcgatgccg cggaagccga gcccgccgcg
     6421 cagcacgtcg gtagccagcg tgcgcgacag cgaggccggg agcacgaagt cgtcggtggc
     6481 gtagctcgcg tgcccgacca ccaccgcggg tgcgccagcg gcgatggccg cgcggaacgg
     6541 gaccaggtcg cgttcgcgca actcgtcgag gctgagcccg acctgcgccg gcccctcgtc
     6601 ggtgtgctgc gtggctccgc cgatcccggg aaagtgctcg acccccgaca gcagctccgc
     6661 cttcctgaac gcggcgacgc ccgccttcgc gaacgccgcc acctcgtcgg gctggtccga
     6721 gaacgcgcgg gtgccgagcg ccccggcctc gttgggcgcc acgtccaccg ccggcccgat
     6781 cacgccgttg aggccgaccg cgcgcagagc gcgggtcgtg tcggcgaact ggtccacggc
     6841 gtccggcagc ccggccagct cgcccggctc gcccgccggc gggaagtcgg ggaacgccga
     6901 ctgctcgccg cccgcctggc gcgcgagcat cagcggcggc tcgtggttct tgcgcgcggt
     6961 cgcgttggcg acggaggccg tcatcgcctc gagctggacc gagtcgcggt agttgcgccg
     7021 ctcgaacacg acgccgccga agtcgctgcg cctcagggtg cggaagaagg tggcggaggg
     7081 gtcgcggccc tggaagccga cgagcatgag ctgcgccgcc ttgcgctcgg cggggagccg
     7141 tgccaccagc cggcggatca cgcccggcac gcgggcgccg ggcagcccgg tcgcgggcga
     7201 gggcacgatc cgcgccagga acgaccccgc cggagccgcc gccgcgcgcg gtgccggtgc
     7261 gggggcgggc ttcttccgat cgtcgccgtc gtcgcccccc gaggcgagca gcaccaccag
     7321 gaccacgccc agcgcgagcg ccagtcccgc ccggagcggc ggcaggttcc tcaggtggcg
     7381 gacgatcgcg gggcgcaccc gccgaaagct atcctcggac cgcgatggcc ggccccgcta
     7441 ccgaccgtgc gggagagccc gcgagcgacc cccggcgcac cgtcgtgatg aagttcgggg
     7501 ggacgtccgt cgccgacgcc gagcgcatcc gccgcgccgc cgggcgcatc gtgcgcgcga
     7561 aggagcgggg cgacgacgtc gtggccgttc tctccgcgcg cgggaagacc acggacgagc
     7621 tcgtccagat ggcccacgag gtctccgcgc gcccacaccc gcgcgagatg gacatgctgc
     7681 tctcgaccgg cgagcggatc tcgtgcgcgc tcgcctcgat ggtcatcaac gacctcggct
     7741 acgacgcggt ctcgctcacc ggctcgcagg ccgggatcgt caccgacgcc tcgcacacca
     7801 aggcccggat catcgacgtg cgcaccgatc gcatcgaggg tctgctcggc gagggccgtg
     7861 tggtgctcgt cgccggcttc cagggcacgt ccgccgcgct caggggagac ggcggcggct
     7921 acgaggtgca cggcgacgtg acaaccctcg gccgcggcgg ctcggacacc acagcggtcg
     7981 ccctcgccgc ggccctgaac gcgcacgtct gcgagatcta caccgatgtg gccggggtct
     8041 tcagcgccga cccgcgcatc gtgcccgagg cgcgcaagct gaactacgtc tcgttcgagg
     8101 agatgctcga gatgtcggcc tccggcgcgg gcgtcctcca gctccgctcg gtcgagtacg
     8161 cgcgcaacca cggcgtgcgc atccactgcc gttcgagctt cgacgacgga ccgggtacct
     8221 tggtcctcat ggaagaggag acgatggaga ggccgatggt caccgccgtc acgcactcca
     8281 cctcggaggc gcgcgtgacg ctgatgagca tccccgacca gcccgggatc gccggccgcg
     8341 tggccaccgc gctcgccgac gcgaacgtga acatcgacat gatcatccag aacgagccgc
     8401 agtcggaggg gcaccgcgcc gacatgtcgt tcacggtgcc gcgcgacgag ctcgagctcg
     8461 cccgcgaggc gctcgagccg ctcgtgggcg acatggggat cggccagatc gcgaccgact
     8521 cggccatcgg gaaggtctcg ctcgtcggcg ctggaatgaa gagccacccg ggcgtcgcgg
     8581 ccaagacatt cagcgtgctc ggcgactcgg gtgtgaacat cgagatgatc tccacctcgc
     8641 cgatcaagat ctcgtgcgtc gtgcgcgagg acgacgtggc accggccgtg cgggcgctgc
     8701 accgcgcatt cgacctcggt gaggacgcga tccgccgcga ggacccgcgc ggcgaccacc
     8761 ggccggtggt gtcctgatgc gcgtcgccct cgttggcgcg accggcgccg tcggctcgat
     8821 catgctcgag gtgctccagg agcgccgctt ccccgccacc gaggtggtgc cgttcgcgag
     8881 cgagcggtcg gtcgggcgca agctcgcctt cggcgacggc acgatcgagg tccaggcgct
     8941 cacggaggag tcgatcgcgg gcttcgacat cgcgatcttc tccgcgggcg gctcgacgag
     9001 cggcgagtgg gcgccgcgct tcgccgcggc gggagccgtc gtggtggaca actcgtcgcg
     9061 ctggcgcatg caggacgacg tgccgctggt cgtctccgag gtcaatcccg acgacctcga
     9121 caaccaccgc gggatcatcg ccaacccgaa ctgctcgacc atgcagatgg tcgtggcgct
     9181 caagccgatc gacgtggtcg ccgggatcga gcggctggtc atcaccacga tgcagtcggt
     9241 ctctggcacc ggtcagaagg ccgtcgaggc gctgcacgac gagaccgagg ccgtgctcgc
     9301 cgccaaggag ctggccaagc ccgaggtgta cgcgcaccag atcgcgttca acgtgctccc
     9361 gcacgccggc accttcccgg agggctccga ctacaccgac gaggagctca agctcgtcaa
     9421 cgagacgcgc aagatcctcg gcaagcccga catcgccgtg tcggccacct gcacccgcgt
     9481 gcccgtgtac acgggccact cggagtccgt gaacgtccag acgcgcgagc cgctgtcggc
     9541 cgacgactgc cgcgcgctgc tcacccgctc gcccggcatc gtcgtgctcg acgatccggc
     9601 gcacggggtc tacccgctcg cgatcgacgc cgccggccgc gacgaggtgc tcgtcggccg
     9661 catccgccaa gacccgtcgc acccgcgcgc gctgaacatg ttcgtggtgg ccgacaacct
     9721 gcgcaagggc gccgcgacga acgccgtcca gctcgccgag ctgctcgtgg agcgcggcct
     9781 ggtccgcgcc cgcgcgcagg ccgcctgagc gccgccgcag cggtcgcatc ccccgggtgg
     9841 cccccggcgc cccccccggc gggacggtta cacactttcg tgactagaaa ccaggcgcgg
     9901 gggcaatagt ccaagcgtgc gggggcacac cgggggctgg ggaggaccgc gacgagcgct
     9961 gctcgccacc gttgccgcgc tcgcgatcct cgctccgtcc gccgccgcgc agtccaccgc
    10021 gcgtccgccg acgcccgagc cgaacacggc cgtcgacatc gcgccgcgcc cgtgcgccgt
    10081 cgagggccgc gaccggtacg agcgcgagtt ctacgagcgc gagggctggc ccggccccga
    10141 ccacgagcgc taccccggcg cctgccagcg gctgcgcttc tcgtacgggc cgatcgccgt
    10201 caagcccggc cagaacgacg tgctgatcga gccggtgaag atcgagaagc cgctgcgcga
    10261 gggctacatc acccgcttca agcccgacct cgtgcgcgcc gacggcgtcg tcccgccgat
    10321 cgagcaggtc cacctgcacc acgccacctg gctcgccgag cccgactacg gcagcggccc
    10381 gttcttcgcc gccggggagg agaagaccgt cgctccgttc ccgaagggct acgggatgcc
    10441 ggtcaagccg agcgacacct ggctgctgct ctacatggtc cactcggcca tccagcagcc
    10501 gatggaggtc tacatcacct acgacatcga cttcgttccc aaggacaagg gcgaggcgct
    10561 cgggctgaag aaggcctacc cgctctggtt cgacgtgcgc ccgtccggct acccggtctt
    10621 caacgtccag cgcgacttcg gcggcgcgga cggcatctgc acctggccga ccgagaagtg
    10681 cgcggcgcac gacccgttcg gcaaggagat cgtcggccag ggccagcccg gcaacggcaa
    10741 gggcaccgac tggtcgttcc cgaagccggg cgagacgctg ggacgggtgg agaacttcaa
    10801 cggcggaacg atcgtcggca tcggcggcca cctgcatccg ggcggcctcc aaaatacgat
    10861 cgacctcgtg cgacccggca acaccgtgcg cgtgaagaag aagatcaagg tcaagacgaa
    10921 gtcgaagaag ggcaggaagc gcaagacgaa gacgaagacc gtctatgtca cggagaagcg
    10981 cgagcaggcg cggatctaca cgggcgacgc ctactactgg gatcgcaagg accgcatgaa
    11041 ggccggcgga ccgccgacct cgtgggactt ctcgatgggc gtcggcggcc tgcccgactg
    11101 gggcgtgcac gtgaagccgg gcgacatcat ccgaagcaac gcgacctacg acaccacgca
    11161 ccagtccacc tacgagaaca tggggatcgt cgtgtcgatg ttcgtgccga acgacgagaa
    11221 cgggaagccg caggcgccgg gggtggaccc gttcgtcact ccgcacgacc cgaccccgac
    11281 ctgcgactcc ggcggcgtgc gcgcgaagcc gccgcggctc tgcgacaggg gcatacccac
    11341 ccacggccac ctcaccgaga acgacaactt cggcggcccc cagggcgagt ggaaggccga
    11401 gcgcggggtg gagaccgacc gcgtcggcat cgccgacttc ctctacgcgc ccggtgacct
    11461 ctcgatgatc tcgatgaccg gcgtgccgac cgtgaagctg ggctcggacc tcaagttcac
    11521 gaactacgac ggcgcggtga tctaccacac ggtcacctcc tgcaagttcc cctgcctcgg
    11581 gcagaccggc acgtcgttcc cgatctccga cggtcagacg agcaaggggc gcaacctcga
    11641 cttcgactcg tccgaactcg gcttcggcac gcccaccatc gggccgacga agcagacgct
    11701 gaactggagc ctgcccgtct ccaaggaggc cgggttcgag cccggggaga tcgtgaccta
    11761 cttctgccgc gtccatcccg ggatgcgtgg ggcattcgag gtccagcagt gagcaacttc
    11821 ggcgaggaca tcccgtttct ggcccgtaac aagccgtacc ggcacgggtg ggccgacctc
    11881 tcggaggcgc cgcccgtcga cgtgctcccg tgctcgaacg acgagtactt cccgccgcct
    11941 cccacccggg agcagatcgg gatcatgcag cttcagagtc gcgagatcga gcgctaccgg
    12001 cgcaggttca acatgagccg gcgcgcgttc gtgcggacgg ccgcggccac ggccatcggg
    12061 ttctgggcga tcgacgcggt gcgccagggc gagtacggca actacggctg ggcggccaac
    12121 accacgaccg acgcctgcga cctcgagtgg gacggcaaga agggcatgga gtcgctcacg
    12181 aacctgcccg gcgagttcat cttcgacgtg cagtcgcacc acgtggacgc ggagggccag
    12241 tggcgcgtga ccaaccccgg gttccatgcg ttcttcgcgg ccctgtggcc gcagtcgtcc
    12301 tgggcgaccg gtgagccggg cggcgtccgt gaggacggct cgatccgcgg cggcggcgag
    12361 ggcggcgagg tcgacccgat tcagaacctc tcgcgcttcc actacctcaa ggagctctac
    12421 ctcgactccg ccacgacggc gaccgtcctg tcgatcgtcc cgacctcgcc cgacaccaac
    12481 aacccgctgc cgctggccga gggctacaag accgcgcgcc tcgtgaacga cctgtcgaag
    12541 gcgcgccgct gcgtgctgca cgcgttcgtg atgccgaacc gcggcagctc gggaacgacg
    12601 aaccccggtg gctctcccat caagccgctt tatctcgagg aggagatgga cctgatgatg
    12661 cagcgcgccg atcagtacgg cgacctgctg cgcggctgga agacctactg cgcctggggc
    12721 gacgtgccca acgcgagtgg ctggacgttc gacacaccgg tcggcgagcg cttcctcgag
    12781 aacgtgctcg cggtgagcaa gaagtacccg cagatcccgc ccgtcgtcgc cacgcacaag
    12841 gggttcccgc tgccggcgtt cgaccagcgc gccgccacgc cgcgcgacat cggccctgcc
    12901 gcgaaggcct tcccgggcgt gcgcttcctc gtctaccact cggggtacga caccggcgac
    12961 cgccagacgg cgtaccgcgg cgacgacgag gcgcgttcgg acacgcacac ggtcgacggg
    13021 ttcatcaaga gcctgcgcga gaaccagtgg gacgcctcgc gcttcgtgaa gaagggcaag
    13081 aagttcggaa acgtccccaa cgtctacgcg gagctgggct ccgtctggcg cggggtcatg
    13141 aacgaccccg accaggccgc gcacctgctc ggcaagctga tcaaccacgt cgggccgaag
    13201 cgggtcgtct ggggcaccga cagcctctgg tacggctcgc cgcagccgga gatcgtcgcg
    13261 ctgcgccgct tcgagttcac ccagaagggc aaggagttct acgggctgcc ctatgggctg
    13321 aacggcgacg tcgaggaccc tcgcaagaag gcgccgcggc cgtcgcgcac gatccgcaac
    13381 gcgatcctcg gctacaacgc cgcccgcgcg tatagcttcg acccggtgaa gatcgccgag
    13441 gagatcgcgt gcgacgaggt gcagcggctc aaggaggagg gctacctgcg cggcggtggc
    13501 accgagaccg agtcggcgcc gctggcctcg cacgtcgcgc cgggtgcccg cacgaaggcg
    13561 gaggtcatcc acaacgtcta tcactccggc gagtg
//
LOCUS       DASVPL010000185         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_376458, whole genome shotgun sequence.
ACCESSION   DASVPL010000185 DASVPL010000000
VERSION     DASVPL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376458"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..273)
                     /locus_tag="VF517_09500"
     CDS             complement(25..273)
                     /locus_tag="VF517_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053216.1"
                     /translation="MRLGSEIIEWGNLLEVVYSAFGAGVGVALAFSIAVAGSTRFADE
                     VRENRMTRAALYAVVAALGLLVCLAAIVLGIVVMTAKG"
     gene            complement(279..1388)
                     /locus_tag="VF517_09505"
     CDS             complement(279..1388)
                     /locus_tag="VF517_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX8053217.1"
                     /translation="MAEDLVFYLVIATALAFDFTNGFHDTANVVATSISTRALPPRTA
                     VAMASILNFVGAFISLEVAATVGSGIVEGEFITLPIVFGGLIGAITWNLITWYFGLPS
                     SSSHALIGGVVGAAYAAEGSKAILGEGLLGKVAVPALIAPTLAFVVAGFAILLCYRIV
                     GRLRPGQVSRGFRGGQIISGGMLALAHGTNDAQKTMGVIVLALIAHGEIAADDFHVPT
                     WVVVTAATAIALGTYLGGWRIIRTMGTRIIKMDTAQGFAAQGGGAAVILAASHAGYPL
                     STTHTISGAVMGAGAARRFSAVRWGVAGNMLVAWVLTIPASAIVGATVYGFVRIFGED
                     SAVGPLLISVGILSGIAWTFARRLRRGSVISAAEA"
     gene            complement(1388..2017)
                     /locus_tag="VF517_09510"
     CDS             complement(1388..2017)
                     /locus_tag="VF517_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEX8053218.1"
                     /translation="MSRLSQVLVPKDRQYFDLFEEAAGNIVRAASLLDDMLRNYPDRR
                     EELTRDILICEQQGDRITHDIIRRLNETFVTPIDREDILVLASSLDDMVDFIEEAADY
                     LGLYKIEAPFEQAQQLSAVLLQCARQLEEAMPRLRNFEDISHFTVEVNRLENDGDRIT
                     RGAMAALFEGGIDPMVVIRWKDIYDRLENAIDAAEKVAYTLEGVVIKNS"
     gene            complement(2122..2805)
                     /locus_tag="VF517_09515"
     CDS             complement(2122..2805)
                     /locus_tag="VF517_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8053219.1"
                     /translation="MHAVLYDIHGNLPALDAVLADAAAAGAERFVLGGDYALFGAWPA
                     ETVERLRTLDAEWIRGNGERWTASPGDAPDNPVVQGAIRASRELLGAGAVAELAALPE
                     EHALGEARFVHGSPVSDVRSFLPEPAGDEDELLAGVAADRLVFGHTHLQFRRRAARGI
                     ELINPGSVGMPFDGDPRAGYALLGDDGDVELRRVAYDQAASAAAVRERFPEFGETVAR
                     RIELAAFAA"
     gene            complement(2813..>3096)
                     /locus_tag="VF517_09520"
     CDS             complement(2813..>3096)
                     /locus_tag="VF517_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053220.1"
                     /translation="ARRITRVTSGFPRMVALGYGKFVRADRIFAVVPIESGDRGDGRR
                     TYVHVEGVAEPLIASRSERAILAELDVALTDAAGLPRAPARRGAGEVPP"
BASE COUNT          458 a         1113 c         1109 g          416 t
ORIGIN      
        1 gcggcgggcc gcccccgctc agggtcagcc cttggcggtc atcacgacga tcccgagcac
       61 gatcgccgcg aggcagacca gcagcccgag agccgcgacc accgcgtaca gcgccgcccg
      121 cgtcatccgg ttctcgcgca cctcgtcggc gaagcgggtc gagccggcca ccgcgatgga
      181 gaacgccagc gccaccccca ctcccgctcc gaacgccgag tagacgacct ccagcaggtt
      241 gccccactcg atgatctcgg agccgagcct caccgcgctc aggcctcagc cgccgagatg
      301 accgacccgc gccgcagccg gcgtgcgaac gtccacgcga tcccgctcag gatcccgacg
      361 ctgatcagca gcggccccac ggcgctgtcc tcgccgaaga tccgcacgaa gccgtagacc
      421 gtcgcgccga cgatggccga cgcggggatc gtcagcaccc aggcgaccag catgttcccg
      481 gccacgcccc agcgcaccgc cgagaaccgc cgtgcggcgc ccgcgcccat caccgcgccc
      541 gagatcgtgt gcgtggtcga gagcgggtag ccggcgtgcg atgcagccag gatcacggcc
      601 gcgccgccgc cctgcgccgc gaacccctgc gcggtgtcca tcttgatgat tcgggtgccc
      661 atcgtgcgga tgatccgcca gccgcccaag taggtgccga gcgcgatggc ggtggcggcc
      721 gtgaccacga cccaggtcgg cacgtggaag tcgtcggcgg cgatctcgcc gtgcgcgatc
      781 agggcgagca cgatcacgcc catggtcttc tgcgcgtcgt ttgtgccgtg cgcgagcgcg
      841 agcatcccgc cggagatgat ctggccgccg cggaagcccc tgctcacctg gccgggccgc
      901 agccggccga cgatccggta gcagagcagg attgcgaagc ccgcgacgac gaaggccagc
      961 gtcggcgcga tcagggccgg caccgcgacc ttcccgagca gcccctcccc caggatcgcc
     1021 ttgctgccct cggccgcgta ggcggcgccc accacgccgc cgatcagggc gtgcgaggag
     1081 ctcgacggga gcccgaagta ccaggtgatc aggttccagg tgatcgcgcc gatcaggccg
     1141 ccgaacacga tcgggagggt gatgaactcg ccctcgacga tcccgctgcc caccgtcgcg
     1201 gccacctcga gcgagatgaa cgcccccacg aagttcagga tcgacgccat cgccacagcg
     1261 gtccggggcg gcagcgcgcg cgtggatatg gacgtcgcca cgacgttcgc ggtgtcgtgg
     1321 aagccgttgg tgaagtcgaa cgcgagcgcg gtcgcgatga ccaggtagaa gacgaggtcc
     1381 tcggccacta cgagttcttg atcacgactc cctcgagcgt gtacgcgacc ttctcggcgg
     1441 cgtcgatcgc gttctcgagg cggtcgtaga tgtccttcca gcggatcacg accatggggt
     1501 cgatgccgcc ctcgaacagc gccgccatgg cgccgcgggt gatgcggtcg ccgtcgttct
     1561 cgagccggtt gacctcgacc gtgaagtgcg agatgtcctc gaagttgcgc agccgcggca
     1621 tcgcctcctc gagctggcgc gcgcactgga gcaggaccgc ggagagctgc tgcgcctgct
     1681 cgaacggcgc ctcgatcttg tagaggccca ggtagtcggc ggcctcctcg atgaagtcga
     1741 ccatgtcgtc gaggctcgag gccaggacca ggatgtcctc gcggtcgatc ggggtcacga
     1801 acgtctcgtt gaggcggcgg atgatgtcgt gcgtgatccg gtcgccctgc tgctcgcaga
     1861 tcaggatgtc gcgggtgagc tcctcgcggc ggtccggata gttgcgaagc atgtcgtcga
     1921 gcaggctcgc cgcacggacg atgttgccgg cggcctcctc gaagaggtcg aagtactggc
     1981 ggtccttcgg cacgagaacc tgggacaggc gcgacatggc tcgacctccg tcagcgaggc
     2041 tcggtttctg ggcttggatg cccggggcgc ggcggcacgc gaccggagca gcggcagtgt
     2101 attgcgggcc gcggtcggcc ttcaggccgc gaaagccgcg agctcgatgc ggcgggcgac
     2161 ggtttcccca aactccggga agcgctcgcg gacggctgcg gcgctcgccg cctggtcata
     2221 ggcgacccgg cggagctcga cgtcgccgtc gtcgcccagg agcgcgtagc cggcgcgcgg
     2281 gtccccgtcg aatggcatcc cgacgctacc ggggttgatc agctcgatcc cgcgggccgc
     2341 gcggcggcgg aactggaggt gggtgtggcc gaagaccagg cggtcggccg ccaccccggc
     2401 cagcagctcg tcctcgtcac cggcgggctc ggggaggaag ctccgcacgt ccgagacggg
     2461 cgagccgtgc acgaagcgcg cctcgcccag cgcgtgctcc tccgggagcg ccgccagctc
     2521 ggccaccgcg cccgcgccca gcagctcgcg gctggcccgg atggcgccct ggaccaccgg
     2581 gttgtcgggc gcgtcgcccg ggcttgcggt ccagcgctcg ccgttcccgc ggatccattc
     2641 ggcgtcgagc gtgcgcaggc gctcgacggt ctcggccggc caggcgccga acagcgcgta
     2701 gtcgccgccg agcacgaagc gctcggcccc ggcggcggcg gcgtccgcca ggacggcgtc
     2761 gagggccggg aggttgccgt ggatgtcgta gaggaccgcg tgcatcgccc gctcagggcg
     2821 ggacctcgcc ggcgccgcgg cgcgccgggg cgcgcgggag gccggccgcg tcggtgagcg
     2881 cgacgtcgag ctcggcgagg atcgcgcgct cggagcgcga ggcgatcagc ggctccgcga
     2941 ccccctccac gtgtacgtag gtacgcctgc cgtcgccgcg gtcgccggac tcgatcggca
     3001 cgacggcgaa gatgcggtcg gcgcgcacga acttgccgta cccgagcgcc accatgcgcg
     3061 gaaaaccgct ggtcacacgg gtaattctac gcgccg
//
LOCUS       DASVPL010000186         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_258547, whole genome shotgun sequence.
ACCESSION   DASVPL010000186 DASVPL010000000
VERSION     DASVPL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258547"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VF517_09525"
     CDS             <1..629
                     /locus_tag="VF517_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053221.1"
                     /translation="VTLSCKQCGDPVEVSANAAPRARIYGRLCGECQAIAKAARRSKD
                     GRRHSAVTRPLAASAQSVAESTSAGRTAAVPQATGRSETSAPRPDELKQLVCADCGGE
                     FSVPRSHGRYPSKCPDCATGRRPGAAPPLRVLAPAGIREEWSGIEYVRVDTKLPRPPH
                     ADLVAMRQVADPVGAGERWEEAPAEDETTVAFWRRWVGPDELVAEAAA"
     gene            633..848
                     /locus_tag="VF517_09530"
     CDS             633..848
                     /locus_tag="VF517_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053222.1"
                     /translation="MAQHVRIGSARLMLLQHDDARKVGFSMRVHAEDAESHAAASTRD
                     PRELRELGDAAYAAAESLEQLLDRGDS"
     gene            845..1036
                     /locus_tag="VF517_09535"
     CDS             845..1036
                     /locus_tag="VF517_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053223.1"
                     /translation="MTARDRQVLELVANRGAVRVPEVASATGLSNAEALDALIELEGG
                     GLLVASVFRITADGREAAR"
     gene            1033..1287
                     /locus_tag="VF517_09540"
     CDS             1033..1287
                     /locus_tag="VF517_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053224.1"
                     /translation="MSASTITRRRRDSRRRAARNRELYGTVDERKERVLEAIDPLGIT
                     PLAVVAERARLPENVTGATLGLLVGDRKVVRHGRRGWERR"
     gene            1284..>1571
                     /locus_tag="VF517_09545"
     CDS             1284..>1571
                     /locus_tag="VF517_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053225.1"
                     /translation="MIAYRDPPLEADRCDRLPRAERWYCEACIASLYPHIRERLERHP
                     DGAPTRRCASCHTPLEDLAGADLLAELAAAVGDKLGTMNFYFGPGARTAVGQ"
BASE COUNT          207 a          561 c          594 g          209 t
ORIGIN      
        1 ccgtcacgct cagctgcaag cagtgcggcg atcccgtcga ggtctcggcg aacgccgcgc
       61 cgcgagctcg gatctacggc cgcctctgcg gtgagtgcca ggcgatcgcc aaggcggcgc
      121 gccgctcgaa ggacggccgc cgtcattcgg ctgtcacccg cccgctcgcc gcgtccgcgc
      181 agtcggtggc ggagtcgacg tccgctggtc gcacggccgc cgtgccgcaa gcgaccggga
      241 ggagcgagac gtcggctccg cgacccgatg agctcaagca gctcgtctgt gcggactgcg
      301 gcggcgagtt cagcgtgccg cgatcgcacg gccgctatcc gtcgaagtgc cccgactgcg
      361 cgaccggccg ccggcccggc gccgcgccgc cgctgcgcgt gctcgccccg gccgggatcc
      421 gcgaggagtg gtccggcatc gagtacgtcc gcgtcgacac gaagctgccg cggccgccgc
      481 atgccgacct cgtcgcgatg cgccaggtcg ccgatcctgt gggcgccggc gagcgctggg
      541 aagaggcgcc ggccgaggac gagacgaccg tggcgttctg gcggcggtgg gtcgggccgg
      601 atgagctggt cgcggaggcg gcggcttaga tgatggctca gcacgtgcgg atcgggagcg
      661 cgcggctcat gctgctgcag cacgacgacg ctcgcaaggt cgggttctcg atgcgtgtcc
      721 acgctgagga cgccgagtcg catgcggccg cgtcgacgcg tgacccgcgg gagcttcgcg
      781 agctcggcga tgccgcgtac gcggctgctg agagcctcga gcagctgctc gatcgaggtg
      841 actcgtgacg gcccgcgacc gccaggtgct cgagctcgtg gccaaccgcg gcgccgtccg
      901 ggtaccggag gtcgcctcgg ccacgggcct ctcgaacgct gaggcgctcg acgcgctgat
      961 cgagcttgag ggggggggcc ttctagtggc ctccgtcttt cggatcaccg cagacgggcg
     1021 ggaggcggct aggtgagcgc gagcacgatc acccgccggc ggcgcgactc gcgccggcgc
     1081 gccgcccgca accgcgagct ctacggcacg gtcgacgagc gcaaggagcg cgtgctcgag
     1141 gcgatcgacc cgctcggcat cacgccgctc gccgtggtcg cggagcgcgc gcgcctgccg
     1201 gagaacgtca ccggcgcgac gctcgggctg ctcgtcggtg accgcaaggt cgtgcgccat
     1261 gggcggcgcg gatgggagcg ccgatgatcg cgtatcgcga cccgcccttg gaggccgacc
     1321 ggtgcgaccg cctaccacgc gccgaacgct ggtactgcga ggcgtgcatc gccagccttt
     1381 acccgcacat ccgtgagcgg ttggagcgcc acccggacgg tgctcccact cgccggtgcg
     1441 caagctgcca caccccgctc gaggatctcg ccggcgcgga cttgctcgcc gagctggccg
     1501 cggcggtcgg cgacaagctc gggacgatga acttctactt cggccccggg gcgcggaccg
     1561 cggtcggaca g
//
LOCUS       DASVPL010000187        32092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_138343, whole genome shotgun sequence.
ACCESSION   DASVPL010000187 DASVPL010000000
VERSION     DASVPL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 32092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32092
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138343"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VF517_09550"
     CDS             complement(<1..211)
                     /locus_tag="VF517_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX8053226.1"
                     /translation="MEISKVGVLGAGLMGHGIAQVSAEAGYDVVLRDIDQAFVDKGRA
                     AIETQLRRAVEKGKKTQKDADAALAR"
     gene            262..1041
                     /locus_tag="VF517_09555"
     CDS             262..1041
                     /locus_tag="VF517_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8053227.1"
                     /translation="MPLVEIEDRGAVRHIVMARAEKRNAMNEELVRGLSEAFRDAAND
                     SSVRIVVVRGDGPMFSSGMDLSDLRLLSEQPENLRPFRGPILAVWNLLEEMTKPTIVQ
                     IHGACIGGAAELALAADMRVMAEDAVVAIPEVRVGLIPDVGGSSRLPAVVGIGRAKEL
                     IMTGKAIDGHEAYRIGFANRIAPADELDAATEALCNELLACAPLAVGLAKRVMDAAAK
                     PALALTLEQEVTCQQLLASSDDYAEGARAFQEKRNPEFAGR"
     gene            complement(1055..1519)
                     /locus_tag="VF517_09560"
     CDS             complement(1055..1519)
                     /locus_tag="VF517_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8053228.1"
                     /translation="MALPEDPAAFVAAAERGINEYDLDATAGAYAPDARLENYVDGSF
                     EAWDGADRVRAAWGAYLDAMRERGFTLRKTLLSAADDTIVNTWTGSLGGRTDAQGIEY
                     WRFDGAGKVREHRMYSLMNVKPSASPLARARMALAYPASALAFLKARKRAGL"
     gene            complement(1530..1883)
                     /locus_tag="VF517_09565"
     CDS             complement(1530..1883)
                     /locus_tag="VF517_09565"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX8053229.1"
                     /translation="MSLDPALAGESYCYLTTTGRVSGEPREIEIWFALNGPTLYLLAG
                     GRERANWVRNLRAEPRVSVRIGAVTAAAAARVVEDPEEDALARRLVHGKYASGPDDMP
                     GWRDTALPVALDLEA"
     gene            complement(1880..2365)
                     /locus_tag="VF517_09570"
     CDS             complement(1880..2365)
                     /locus_tag="VF517_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX8053230.1"
                     /translation="MANAFSTIVGNLDYPMFVATTIGSGGERAGCLVGFATQASIDPS
                     RFLVCVSDKNRTYRVLEGANALALHVVPEDAEDVVELFGGETADDTDKFDQCEWREGP
                     EGLPILERCPSWFAGRILERHRLGDHVGHLVEPFAGEAEYEGRAYPFSRAKRIDPGHE
                     A"
     gene            2450..2899
                     /locus_tag="VF517_09575"
     CDS             2450..2899
                     /locus_tag="VF517_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX8053231.1"
                     /translation="MNDKSASRTPAQLYALVFGATLVLAGILGWFYSSSFGSPGEVDG
                     VLGILDVNGWHNIVHMATGVLGLLALGYSAARTYALGLGAVYVLVAIWGFAVGDGGQI
                     LSIIPVNTEDNILHLLIGLAGLGAYAATPAVEKPTTAHRTDATGAAA"
     gene            3023..3523
                     /locus_tag="VF517_09580"
     CDS             3023..3523
                     /locus_tag="VF517_09580"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8053232.1"
                     /translation="MDAEPAADVEAVAVVPAPPEAVFAFLDDLGNHWIVADRLVEVLD
                     LHTPDGGPAAGGRVRLRGPLGVHRTVTTRVVASKAPRLLIGTAEIGSGTRARVSWALA
                     EHRGSTRVRLAAAVERAARVDRALLALGGRRWLRARFESTLAGLVEEFAARAPAQPAA
                     HSSTVP"
     gene            complement(3490..4638)
                     /locus_tag="VF517_09585"
     CDS             complement(3490..4638)
                     /locus_tag="VF517_09585"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8053233.1"
                     /translation="MEALAAMERGSASPGDVAAAEWGARRLAEAGARDVRVERFRYQR
                     TIAERSVPHLAAGIAAARAGGVAGAAVALASLVSFELDFGGRSQWLRRLVPAGEGANA
                     VARVPAAGESSRTLVLVAHHDTAHTGIGWRNPELIGIGTGDRLRELTGREPEPDVMDS
                     PATAPKLAFAAVAAGSLLRARALRAAGGASLAVALAMALDVARSPSVPGASDNATGVA
                     AVLAMVERFAAEPLPGTDVIAVLTGCEESGMGGMAHWLRGDGGRLDPSSTLVLGLDTL
                     GAGEPIVARAEGSIRPWRYRDEDLDIADRGAARAGIEPPRRVQLGGWTDPLLAVHAGL
                     PTISLLSATGNRFTNYHQPTDTPDRVDWDSVAACLRCAYGTVEEWAAG"
     gene            complement(4668..5579)
                     /locus_tag="VF517_09590"
     CDS             complement(4668..5579)
                     /locus_tag="VF517_09590"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX8053234.1"
                     /translation="MADPIQVREVGPRDGFQNEPEVIATDDKVRLVEMLARTGLRRLE
                     VTSFVRPDVIPQLGDAAEVLTRADIPDDVSVSVLIPNEKGLDNALPLRERFHEVNGFL
                     SATETHNHKNVNRSVEESLAGLERVFGRARVEGLRCEGVISVAFGCPYEGEVAPERVF
                     EIAQRLAAAGCAEVAFGDTTGMANPAQVREFMAAARERLPEVELTAHFHNTRGQGLAN
                     VLAAIESGIRSFESSFGELGGCPVPRGATGNIATEDLVSMLHEMGHPTGIDLEALVDC
                     AREAQRVLGRPLSGHVIVAGPVDWHRS"
     gene            complement(5746..6492)
                     /locus_tag="VF517_09595"
     CDS             complement(5746..6492)
                     /locus_tag="VF517_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8053235.1"
                     /translation="MRLEGRIALVTGGASGIGAATCRRLAAEGARVAVTDLNLDGART
                     IAEEVSGQPYELNVAEDESVRAAIAAAESDLGPIDVLVNNAGYDEFGFFVNTDQDMWQ
                     RVLGVNLVGVIACTHAVLPGMQERKRGRIVNVASEAGRVGSPGSAVYSGAKGGVIGFT
                     KAIAAEAARYDVTCNAVAPGPIETPLLMAARHLGDLGERLINGMVSKTVLGRLGQPDE
                     VAATIAFLASDDASYVTGETLGVSGGMGRI"
     gene            6525..7211
                     /gene="npdG"
                     /locus_tag="VF517_09600"
     CDS             6525..7211
                     /gene="npdG"
                     /locus_tag="VF517_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX8053236.1"
                     /translation="MEDTASVPIVGGTGALGFGLALRWARAGVPVVIGSRSAERAEEA
                     AGRLRERVPDAQAEGLENAEAVRRGPIVFLTVPFRNQSENLTNLKEALEPGQILVDAT
                     VPLAAAVSGKATRLLGVPQGSAGQQAQEMAPEGVRVVSALHTVSAATLGELDVELDED
                     IPIAGDKRDDKRRVAELIARIPGLRPVNAGLLESARLIEGLTPLLISVNARYKTHAGV
                     KFTGLPDELW"
     gene            7205..8140
                     /gene="cofD"
                     /locus_tag="VF517_09605"
     CDS             7205..8140
                     /gene="cofD"
                     /locus_tag="VF517_09605"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX8053237.1"
                     /translation="MVVVLAGGTGGAKLARGMLDAADALTVVANTADDTEVYGVHVSP
                     DPDLVTYWLADAIDARGYGIDGDTWEVMGALEAAGRETWFRLGDRDLAMCLIRTELLA
                     AGERLTAAHDAVVRAMGVRARVLPMSDEPVRTFVGSGGRARAFQEFMIVDRAAGPIES
                     VELRGLESARPGPEVLEAIASAEAIVIGPSNPVISIGPILALPGMRAALRAASAPVVA
                     VSPFVEGHAVKGPTEPFMEHFELELGAAGMAAAYGDVLDGIVADEGFGGAPVPVLVTD
                     TLMGDATARRRVATETLEFARSLSGGRASAGPDRR"
     gene            8168..8851
                     /gene="cofC"
                     /locus_tag="VF517_09610"
     CDS             8168..8851
                     /gene="cofC"
                     /locus_tag="VF517_09610"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX8053238.1"
                     /translation="MRTLAIVPVKSFDEAKQRLAASLARGSRSSLAQAMFSDVLAALD
                     RARSVEAVAVVTADQAAQQLAHGRALVLHDSAQAGQSDAVEIGIRHAVAAGYPRVLLV
                     PGDTPLMDPAEVDALLDASEREGLGAAIVADRHGTGTNALVLTPPGAIAPAFGPGSLE
                     RHVRLAEEAGVAHRVAAVPSLEHDVDTPEDLAALRSVLSGVRGRGQRTWGALHQIDHS
                     RATPVEVRA"
     gene            8869..9597
                     /gene="cofE"
                     /locus_tag="VF517_09615"
     CDS             8869..9597
                     /gene="cofE"
                     /locus_tag="VF517_09615"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX8053239.1"
                     /translation="MADLPEVRPGDDLGALIADAAPADLASDDVLVVAHKVVSKAEGR
                     LRRLEEVEPGERAVELAAPYGKDPRAVQVVLDESAELLRAERGVIVSVTRHGFVCANA
                     GVDASNAPEGTLVMLPLDPDSSARRLRARLGELRGVRPAVVVTDSFGRAWRTGQTDVA
                     IGAAGLVAVDDWRGRRDSVGRELNATAIAVADSIAGAADLARTKDSRRPAVLVRGLGH
                     LVTEDDGPGAAPLRREPDQDLFRR"
     gene            9729..10469
                     /locus_tag="VF517_09620"
     CDS             9729..10469
                     /locus_tag="VF517_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053240.1"
                     /translation="MPAAFTAALIAALLGAAPASAQLPTDLPAGWGGGSSAQRGDDRG
                     HWGGWDDQNEDEPADEPAPADDPAPADEPAPPSGWIDGGGLLPGGGDEPGAELLPPGG
                     ARPIVKGRLAALGTNGAAYAPSKAPRQVKLAIWAANQLRQKPYKWGGGHARWSDSGYD
                     CSGSVSFALHAAGLLGGPQTSGTLTRYGKGGLGKWISIYANKGHVFMVVAGLRFDTSP
                     HGSGESGPRWRPTGRPAGGFKVRHPAGL"
     gene            complement(10609..12027)
                     /locus_tag="VF517_09625"
     CDS             complement(10609..12027)
                     /locus_tag="VF517_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053241.1"
                     /translation="MGRGLAAAVATALVALAAAGPAAGQTPCTADLPATAPPPQPGGQ
                     LRYGIYPGGPAGQLGGIPSSSRRENQDKIFAALAQLKPTGGPFAVHLYRAYYTDEEQE
                     KEERTHRRLVDLYTSRGYGTEIVIRYRRDDDVAGFAAFVRGVVRRFGSNPLVKGFQIT
                     NEVNFEVSPDSSDGAYKGARDALIQGVIAAKDEARSLGYSQVQIGFNWVYRMDPQTER
                     SFWEYLGSAGGERFVKSLDWVGLDAYPGFFVPPRLEPSQTGDFMVHAMSTLRKCSMPL
                     AGIPDTVPIHIEENGYPTSPDRPYEDQAVAATSMLDAVSDYARVYNVTDHFWFDLRDA
                     ESDNPNPQQQYGITRDDYTPKPAFDVLRDRFARYMAPAPAAPPVTAPPPPRAAVVKLR
                     LRCSSRGVVAYTTGSDAGSASRVRFAMRGRKVEDSKAPFSVKVRLRPSARSQRFRAVA
                     KVRVGAAKPLVRVKHARCTVRR"
     gene            complement(12046..12447)
                     /locus_tag="VF517_09630"
     CDS             complement(12046..12447)
                     /locus_tag="VF517_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8053242.1"
                     /translation="MSDYTVKRIDEMEAVYGGGFKRARAELGVTSFGMQVLDFPPNSD
                     QHPEHDHAEEGQEEVFVPLSGRGELVVDGDRHVLEPGMMVRVGPAARRAITTGDAPLR
                     LLALGGHPGRPYQGKEVTELGAPDPLAQSRG"
     gene            complement(12500..14674)
                     /gene="cofH"
                     /locus_tag="VF517_09635"
     CDS             complement(12500..14674)
                     /gene="cofH"
                     /locus_tag="VF517_09635"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:TIGR03551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX8053243.1"
                     /translation="MRRRVTFSRNFTLSLSRTCRCYCKYCAFATHRAHLYAPDEVEKL
                     LDDAARRGVKELLVLTGEAPDVNPQVAAKLAEYGHEDFTSYVAWACERGLERGLLPHT
                     NIGVASREDLARLREVTASQGLMLESVNPDLVVHQGSPTKHPELRLDCIRAAGELRIP
                     FTSGILVGIGESREERVAALEALAAVHAEHGHIQELILQNFVPHPRYYGAEPAAIADE
                     AQRSGAHGEPAAMPPWATPVELDDMRELVREAKRLMPDVGVQIPPNLSDWWLPLVEDG
                     ATDLGGLSANGDHISPEHPFPSPHRMRKELAPRGYALTERLCVYPQYMTPEWIEQGVL
                     DVIKSRYWSFIPRRGSGRREERAIRRDLVPGAIARGRDGVELSPEELTALFAETRPEA
                     IEDMRQAADELRAELAGDEVTFVVNRNINLSNVCVVGCAFCGFGQGRRSPDAYEHDRE
                     EFARRVDEALDYGATEICIQSGIHPEWTLETYEDWLRYAKELAPEIHLHAYSAMEVDR
                     MCEVSGLPPREVFERLRAAGLGSTPGTAAEVLDDGVRERISPNKLPVGRWVEIIEASH
                     ASGLRSSATVMFGHVEEPWELAEHMRVVRGLQERTGGFTEFVPLSFIPFHTLLGRTHG
                     IEEISREENLKHTAAFRLALGRTVPNLQASWVKMGLDAATESLRWGVNDLGGTLMEES
                     ISRMAGSYHGTMVDPDDLIAAARAAGRAPVQRDTLYRVLQTY"
     gene            complement(14725..15552)
                     /locus_tag="VF517_09640"
     CDS             complement(14725..15552)
                     /locus_tag="VF517_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX8053244.1"
                     /translation="MAETGNAAEGSVVNAATVRIPDRGPAAYVAEFIGTFALVFAICA
                     AVVLFVPGPPDPSPTGAPAQSFFQDFAVVGLVHVLVLFFLIQTMAVMSGAHFNPAVTV
                     AMTAVRQIKPIDSAIYVLMQLAGGTLGALAVKGLYNEIGDQNGQNFGAVGFNADRLDN
                     TLQGMGLEALGTFFLVWAIMGVAVNPSAVKDWSGLVIGGTLGFAVMIIAPMTGAGLNP
                     ARALGPAIVAKSFGDEGKWLLLYVLAPVLGALLAVFLYSYLMVTHGKKGTGGAEPVG"
     gene            complement(15688..16449)
                     /locus_tag="VF517_09645"
     CDS             complement(15688..16449)
                     /locus_tag="VF517_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8053245.1"
                     /translation="MSRTAVITGASSGIGEATARRLGDEGWKLLLVARRADRLEGLAG
                     TLPDASVLALDLTDDDAPARVREAVEERFGGQLHLLVNNAGANWRAYFGDDDGGWANV
                     ERVLEVNLHAPIRLTEALLPVLRSSAPSSIVNVASVAARIPYPRGGQYAASKFGLTGW
                     SESLRYEEEAHGVHVGVVFPGFVATEGFPQEQLVGKAATRWVVSTPDRVAEAIADAGP
                     GGKPERYVPRPWLIPVILRTLAPGVVRRIARGMGG"
     gene            complement(16446..16784)
                     /locus_tag="VF517_09650"
     CDS             complement(16446..16784)
                     /locus_tag="VF517_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053246.1"
                     /translation="MARVALLCPDLLFGSRIEGGLAAVGHEVTRYDREPAAREAAAAE
                     ADVLVVDLNAEAFDGPALGALAGVGKLGFYGHTDVDTKQRAEAAGFDLVVPRSRMARE
                     MATLVERVAA"
     gene            complement(16784..17146)
                     /locus_tag="VF517_09655"
     CDS             complement(16784..17146)
                     /locus_tag="VF517_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053247.1"
                     /translation="MPLADDFDRILGSLPPDWTHLELDLRIFDEERYVEAASLLVQVN
                     AQPYSKHDWHWRLHSMREFGHAAAPQTVHGTLAVLDGEGIEGELAMRDARVGRVEVTP
                     MWGRPESVRQEHRRRRAQ"
     gene            17171..18079
                     /locus_tag="VF517_09660"
     CDS             17171..18079
                     /locus_tag="VF517_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053248.1"
                     /translation="MILASEYELLREDAGAVERRRGKLLLEGAETAAFLQGQVTNDVE
                     GLAPGTGCYALLLGHKGKVRFDMRILRGPDWIWIDCAKGALPVLQHTIRTYGIGRDMR
                     SRDVTEERSVVSVIGPRARAALGVDVPEEEHAFVDDGGVLHVATDVGVDVIGPPEALA
                     PEIPAVSEEVAEVLRIESGRPRFGLDFDGDTIPQEAGLNERAVSFTKGCYVGQETVAR
                     LHYKGKPNRHLRGLRLSEPAERGVELRLGERVVGALGSAAVSPVHGPIALALVRREAE
                     PGAVLEVGADGVTAQVVELPFRKSDG"
     gene            18133..19992
                     /locus_tag="VF517_09665"
     CDS             18133..19992
                     /locus_tag="VF517_09665"
                     /inference="COORDINATES: protein motif:HMM:NF045524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8053249.1"
                     /translation="MPLRPILTLIACLVCAAPAAADAPVRASSAGAQSEARATLDRAQ
                     ALLDGRGVESGKELTPVLKDLSLRLKALPEGDRREARAILARPTVGQAQAGEEAYRPG
                     STEHRICTAHFCIHWVTSPDPEDPDPPPLDSNDGDAIPDYVQTMSQVFENVFAVENIG
                     MGWREPTPDDTRGGDFNKVDVYIKQLGDQGIFGYATPDPGQESNSQAAYLVMDDDFTP
                     AEFPRYSEPLPPMQVTAAHEYNHVLQFGYDVLQDSWMFESTAVWMEDKVYDDINDYVS
                     YLGPWTQLTQVPLTAFNPGDLADPMNVKVYGDAVWNRWIDEHYGADTVRGAWERSLET
                     RPLSFAPDAYDASLLAKGTTFFDSFTRFAADTAEWRSAAGPFEEGPTWPDVQRASKTN
                     LAPGGPGVEGRLDHTTFALLNVTPTADERIKLIGSLPRGVRGAFALVGREGPIDSGTP
                     VVELKRLTSGGQTSVELVNPGRFERITAVLINGDVTQNGFSQFTGDWAYSKDNQPVLA
                     HVSSDYTPPRVRKRSPASGAKVARSAPVVVTFSEPMQNISTKTLDLIGPGGRHVSARV
                     SYDAAKRRARLVPKRKLDPRRRYTVKLGSTVVDLGDNPLPAKDRSWKFSTRSK"
     gene            20076..20279
                     /locus_tag="VF517_09670"
     CDS             20076..20279
                     /locus_tag="VF517_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053250.1"
                     /translation="MTTYISDQRARRLAELDARERQAWETYSESVRGLEGRDYEETED
                     QSWDRLQMKLRELEDERQLVAGD"
     gene            20399..21256
                     /locus_tag="VF517_09675"
     CDS             20399..21256
                     /locus_tag="VF517_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX8053251.1"
                     /translation="MRDTALDEVADPRLLRELIPAAEPGRDLDDWGRSERVFSLMEPV
                     LNFYYRYWFRVEQEGIEHVPDEGGALLVSNHSGALPPDAPMIMQTLRNEHPTSRPLYM
                     LGENWFKGYPGVGMLANKLGLVSAHPANAQRLLGEEGRLVLVFPEGQKGTRKLYWQRY
                     RLRRFGRGGFVKTALRAGVPVVPVAVVGAEEAMPIFAHVPLLQRLTGLVYFPVSHAFP
                     HLGLAAGLLYMPAKFKIRFLEPVHFDGYGAEDADDLELVQSLAEGIRERIQREVDQMI
                     SARTSVWFG"
     gene            21343..21714
                     /locus_tag="VF517_09680"
     CDS             21343..21714
                     /locus_tag="VF517_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053252.1"
                     /translation="MSGCGDDDDFANDPRPPSPVQLSGVITNSDVSVEPRKIGAGPII
                     LIVSNQTEQSHTVTLEGPSGSEEVGPINPLDTGRIQHNLEQGSYQIKAGSDKAVDEEI
                     QPAVLSVGPKRESSSNSVLLP"
     gene            complement(22139..23764)
                     /locus_tag="VF517_09685"
     CDS             complement(22139..23764)
                     /locus_tag="VF517_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053253.1"
                     /translation="MEAATPLRTATIRTLGDDRLVVDGLVIQDECAVRLVREREEAGD
                     DPVRAVADAIEIGARVLDREQAGANADFVKSEFDKVSREVEAAFTERAREVSEQLEKQ
                     MDAVFGPESGQLTKALERHFSDDSAGAVQNRVKDVINEVMQRSREDLLKQFSSADGQN
                     PLAEFKAGTLRQLREAGERQDSNLRTLNEQLTALKIELQSLHAEKEKAEEVDAERERG
                     SAKGRTFEEAVAEALERIAAARDDSCDAVGDVTGSTGKVGDVVVELDACNGPARGRIV
                     FEAKSGKALTKPKAWQQLNAALAQRDADFAVLVVPSEERLPAKTRPLTEYNGDKLLVA
                     FDPEGDSTLPLEVAYSLARARVLMARGGDDVVDSSAIRDVVERALAVVDDARKIKSQL
                     TGSKKGIEVAYSMLDEMENRVRDYLRDIETLAAAGADEGAAADGGVAAGGAAADGGAA
                     AGGAGAAEGAAADGGATVDGGAAAGAAADGGATVDGGATVDGGAAEGAATAREPARAA
                     EPAAAWEPPATEPARSRAPAPALSVIPGQEQLV"
     gene            23860..24435
                     /locus_tag="VF517_09690"
     CDS             23860..24435
                     /locus_tag="VF517_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8053254.1"
                     /translation="MQATGKIAKRGGGATDGGAATDGGAATGGGAATGGGAATATPAT
                     LTEDLATFVKYLMHSHGEDFFKAVGELDLSFTQVRILGVLARDVERASVKELADNLGL
                     SLPAVSRSIEGLVQRGYVTRAEDSDDRRMKQIAATGDGTALVARLMELRVASAADFLA
                     TLSAEERAGLAAALEPIVAREDVAALRRKVR"
     gene            24453..26024
                     /locus_tag="VF517_09695"
     CDS             24453..26024
                     /locus_tag="VF517_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053255.1"
                     /translation="MRLNEDNRKWWTLAAMCFALFMVMLDNTVVNVALPSIQDDLDAS
                     LSALEWTVNAYTLTFAVLLVTGGRLGDIFGRRRMFLFGVVVFAISSATIALAPTQAWL
                     VASRAFQGVGAAFMMPGTLSIISNTFPAHERGRAIGTWAGVSALALALGPVVGGALAE
                     HVSWRAIFVLNLPVAAGAVAVTLFATRESYDRTVSRAVDYAGIATISVGLTALVLALV
                     EGNQWGWDSPEVLALFGTAAVALVAFVWVELHGRSPMVEFSYFRSRSFLGANAIAFIV
                     SFAMLAMFFFMALYMQNILGYSPLEAGIRFLPSTVVIIFTAPIAGRLADRIGPRPLMA
                     TGLAAAAVALWMQSRVTVDTTYSDLLASFSLMGLGMGLTMSPMSTAAMNSVSADKAGA
                     ASGILSMFRMVGGTFGVAVIGALFQSLAHDRVTDAARAAGLPRSMENELVENVGSGRV
                     ADIAQGLDPVAAERLADGMREAFVNALGGALTLSAAVAAAGAVIALVLVEPKRAHRPV
                     DVARQPEAVEPAGAQ"
     gene            26121..27371
                     /gene="ccrA"
                     /locus_tag="VF517_09700"
     CDS             26121..27371
                     /gene="ccrA"
                     /locus_tag="VF517_09700"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX8053256.1"
                     /translation="MAVDLKNVTDVATEPGTLPETMAAWVIRQEREGEPTDAFQLEEI
                     EVPEPGALEVVVRVMAAGVNYNNVWAALGQPVSVFRYHPEDDHHIGGSDASGIVWKTG
                     PGVTRWKVGDEVIIHCNQASYEDPEVHGLDPMAAPSQKIWGYETSWGSFAQFTKVQAQ
                     QLIPKPENLTWEEAASYGLTYFTAYRMLITRCNIQAGDRVLIWGAAGGLGVFAVQLCN
                     LAGAVSCGVVSSAEKGALLEQIGITGWIDRNDHAGMMRKGGESPEEEKARFKASRAFG
                     NAVKEILGGDPDIVFEHVGQATFPTSVLVVKPFGKVVICAGTTGYNLDFDVRYLWMRQ
                     KEILGSHFANAWECTKANELIASGEIRPVLWRTMGFDGVAEAHQIMKENKHLGKIAIL
                     VGAEETGLGKTAEGPGAIYAEVGA"
     gene            27371..27697
                     /locus_tag="VF517_09705"
     CDS             27371..27697
                     /locus_tag="VF517_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053257.1"
                     /translation="MMQADENIAVVYWSINPFRGDKFEDAWRPAARAALDYGATSWML
                     FRSQDDPLSFIQLASFPTKKDFDRYWFSEEIAEARAAASGLYQVPVIPKWLRVVDSGE
                     LARVES"
     gene            complement(27694..28287)
                     /locus_tag="VF517_09710"
     CDS             complement(27694..28287)
                     /locus_tag="VF517_09710"
                     /inference="COORDINATES: protein motif:HMM:NF037271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8053258.1"
                     /translation="MTTTLAEATRGLLRDTVLDAVGELLAGRPWSDVTMGEVAERGGV
                     SRQTLYNVFGGRRELAQAYVMREAERFMAATEEAIRADAHDPRAALASATELFLAAAA
                     ENPLVRAISSSDEGDELLPLVTTRGGPLIADVTARLADVIVATWPALPLRETRTMVES
                     LVRLAISHAALPTASPRATADAIVAILGPYVDEMLGD"
     gene            complement(28291..29454)
                     /locus_tag="VF517_09715"
     CDS             complement(28291..29454)
                     /locus_tag="VF517_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEX8053259.1"
                     /translation="MTTASVNQPLTATWTDPKRYLWLLGLIVPLAPFLAFGLAELTGL
                     GVMWWFGPALVFGLFPFLDHAIGKDSSNPPDSAIKWLERDPYYRWCTYVFIPLQYGSL
                     LLACWLWTSGDLSLLESLGLAMTIAMVSGIAINTAHELGHKRKSHERWLSKVALAQTG
                     YGHFFVEHNRGHHVRVSTPEDPASSKLGESFWAFLPRTVMGSLRSALHLERERLADAG
                     ERFISPRNDVLNAWAMTVVLFGALVAVFGVEILPWLAIQAVVGFSLLEVVNYLEHYGL
                     LREKRDDGRYERTRPEHSWNSDNVASNVFLYHLQRHSDHHANPMRRYQALRHYDGLPE
                     LPSGYATMILLAYLTPLWRRVMDPKVVAHYGGDVTRANIHPRKRARYLARFGA"
     gene            29543..>32092
                     /locus_tag="VF517_09720"
     CDS             29543..>32092
                     /locus_tag="VF517_09720"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HEX8053260.1"
                     /translation="MRRGIARAALAAAAASLIGLAAAMPASADLNTLKASCQQRDAAD
                     GDTANGITLPFTFCDDGVPDVGGRTPNIGAVKAVAVPQRYDGFAGLPPKVAPDPNAGA
                     DLDGNIALDIDVSLPDPARHPPPPGGYPLVALMHGCCAGNKTSWEAPAVDAPREQWHY
                     NNAWFASRGFVVLNYTARGFVDQNDHGSTGETQLDSRLYEINDFQHLAGQLADDPYFH
                     VDPDRVVATGGSYGGGWTWMAITDPTWRSPAGRDMRLVAAAPKYGWTDLAYSLVPNGT
                     HFEGVLPTTDPERAAEPFGTPKASIVAALYTSGRTGIPPGTGPHSTFAPNIDQALACL
                     EADEPLASTPTCGAALDVTLPGFRRDRSAYYQSRFFERIASEPQSRVPIFSAGTFTDN
                     LFTAIEHRRMVDRIKSLVPEYPVQEFYGDYNHFVQNKPKEWGDVCGGDRHVCRFADYP
                     SGDLNAEPAGRLRTGATTRLNAFLDHYVKPQGNPSQPRPAFDVTGSLLICPQNASAEF
                     PADEPGERFTAPTFDELAPNTLTIAAAGAQATSNAAADPHAAQAEPVSNSVANGGRCP
                     VATTPAGAGTAVYDSDPLPRDYTMLGLSRVHATHTGSGDDIQLNARMYDVFPDGSAVM
                     MDRGAHRVTDANGTTVLPIQGNGWRFPAGHRIRIELTQNDAPYMRQSNRPSALLISGM
                     KLEVPVREASATMGAEAAGSEPPGVDLSSPRLASDTSRDPRFRLGLRSTGGASDHYEL
                     EVRNLRSTTWKRLSSNLRVPVFSFAGYFGSAYEFRARAVGRFGERGAWDTARTVVPFD
                     DSRRRSKPSFGRGWSHVRNASAWGQRLSRASGKGRGMRLAFKGAAKVYLIAR"
BASE COUNT         4429 a        11878 c        11358 g         4427 t
ORIGIN      
        1 cgcgggcgag cgccgcgtcg gcgtccttct gcgtcttctt gcccttctcg accgccctgc
       61 gcagctgcgt ctcgatggcc gcgcggccct tgtcgacgaa cgcctggtcg atgtcgcgca
      121 gcacgacgtc gtagcccgcc tccgcgctca cctgcgcgat tccgtggccc atcagaccgg
      181 cgccaagcac gcccaccttc gagatctcca ttagcctccg tcttcctgtt ttcgtcagcg
      241 ctctgagcgc gaggagccta catgccgctg gttgagatcg aggatcgcgg cgcggtccgc
      301 cacatcgtca tggcgcgcgc cgagaagcgc aacgcgatga acgaggagct cgtccgcggc
      361 ctcagcgagg cgttccgcga cgcggcgaac gacagctccg tccggatcgt cgtggtgcgc
      421 ggcgacgggc cgatgttctc gtccggaatg gacctctccg acctgcgctt gctctccgag
      481 cagcccgaga acctgcgccc gttcaggggg ccgatcctcg cggtctggaa cctgctcgag
      541 gagatgacca agccgacgat cgtgcagatc cacggcgcgt gcatcggcgg cgccgccgag
      601 ctcgccctcg cagcggacat gcgcgtgatg gccgaggacg cggtggtcgc gatccccgag
      661 gtgcgcgtcg ggctgatccc ggacgtgggc gggtcgtcac ggctcccggc cgtggtgggg
      721 atcggcaggg cgaaggagtt gatcatgacc gggaaggcga tcgacggcca cgaggcgtac
      781 cggatcggct tcgcgaaccg gatcgccccg gcggacgagc tcgacgccgc caccgaggcg
      841 ctctgcaacg agctgctcgc gtgcgcgccg ctcgcggtcg ggctcgccaa gcgcgtgatg
      901 gacgcggcgg cgaagccggc gctcgcgctc acgctggagc aggaggtcac ctgccagcag
      961 ctgctcgcgt cgagcgacga ctacgccgag ggcgcgcggg cgttccagga gaagcgcaac
     1021 ccggagttcg cgggccgcta ggcggctcgc cgcctcagag cccggcgcgc ttgcgcgcct
     1081 tcaggaacgc cagcgccgag gccggatagg cgagcgccat ccgcgcccgg gccaacgggc
     1141 tggccgacgg cttgacgttc atcaggctgt acatccggtg ctcacggacc ttgcccgcgc
     1201 cgtcgaagcg ccagtactcg atcccctgcg cgtcggtgcg gccgccgagc gagccggtcc
     1261 aggtgttcac gatcgtgtcg tcggcggccg agaggagcgt cttgcgcagc gtgaagccgc
     1321 gctcgcgcat cgcgtcgagg taggcgcccc acgcggcgcg gacgcggtcc gcgccgtccc
     1381 atgcctcgaa cgagccgtcg acgtagttct cgagccgggc atcgggggcg taggcgccgg
     1441 cggtcgcgtc gaggtcgtac tcgttgatcc cgcgctcggc cgcggcgacg aaggcggctg
     1501 ggtcctcggg cagcgccatg cgccaaaggc tacgcctcca ggtcgagcgc caccggcagg
     1561 gcggtgtcac gccagccggg catgtcgtcc ggcccgctcg cgtacttgcc gtgcacgagc
     1621 cggcgggcga gcgcgtcctc ctcggggtcc tccacgacgc gcgccgcggc cgccgccgtc
     1681 acggcgccga tccgcacgga cacccgcggc tcggcccgga ggttgcgcac ccagttcgcg
     1741 cgctcgcgac cgccggcgag caggtagagc gtcgggccgt tgagcgcgaa ccagatctcg
     1801 atctcgcggg gctccccgct cacgcgaccc gtggtggtca ggtagcagta gctctcgccg
     1861 gcgagtgccg ggtcgaggct catgcctcgt ggccgggatc gatcctcttg gcccgcgaga
     1921 aggggtaggc cctcccctcg tactccgcct cgcccgcgaa cggctcgacc aggtggccga
     1981 cgtggtcacc gagccggtgg cgctcgagta tccggccggc gaaccagctc gggcatcgct
     2041 cgaggatcgg cagcccctcg ggtccctcgc gccactcgca ctggtcgaac ttgtccgtgt
     2101 cgtcggcggt ctcgccgccg aacagctcga ccacgtcctc ggcgtcctcc gggacgacgt
     2161 gcagcgcgag ggcattcgcc ccctcgagca cgcggtaggt gcggttcttg tcggacacgc
     2221 agacgaggaa gcgcgacggg tcgatgctcg cctgggtggc gaacccgacc aggcagcccg
     2281 cgcgctcgcc gccggagccg atggtggtgg cgacgaacat cgggtaatcg agatttccga
     2341 cgatcgtgct gaaagcgttt gccatgtcag gtgtatctat cgagacatcg gattccgcgt
     2401 ccactccgta taggcggttg accggacgcg aatcactgga ggttttgcaa tgaacgacaa
     2461 gtctgcatca aggacgcccg cgcagctcta cgcgctcgtc ttcggcgcga cgctggtgct
     2521 ggccgggatc ctcggctggt tctactcgtc gagcttcggc tcgccgggcg aggtggacgg
     2581 ggtgctcggc atcctggacg tgaacggctg gcacaacatc gtccacatgg cgacgggcgt
     2641 gctcgggctg ctcgcactcg gctacagcgc cgcgcgcacc tatgccctcg ggctcggcgc
     2701 ggtctacgtg ctggtcgcga tctggggctt cgccgtgggc gacggcggcc agatcctgtc
     2761 gatcatcccg gtcaacaccg aggacaacat cctgcacctg ctgatcggcc tcgccggcct
     2821 cggcgcgtac gccgccacgc cggcggtcga gaagccgacc acggcgcacc gcacggacgc
     2881 caccggagcg gccgcgtaga gacgggtatc cgccgggcgc cccacgcggg cgcccggcgc
     2941 gccttcgcgg cggctcggcc tccgcgccgc gcccccgccc gccgcgtaac ctccgccgcg
     3001 taacctccgc cccgtgccct ggatggacgc cgagcccgcc gcggacgtcg aggccgtggc
     3061 cgtggttccc gccccgcccg aggccgtctt cgccttcctc gatgacctcg gcaaccactg
     3121 gatcgtcgcc gaccgtctcg tcgaggtcct ggacctccac acgccggacg gcggccccgc
     3181 cgccggcggc cgcgtgcgac tgcgcgggcc gctcggcgtg caccgcacgg tcaccacacg
     3241 cgtggtcgcg tcgaaggcgc cgcggctgct gatcggaacc gcggagatcg ggtcgggcac
     3301 gcgcgcccgg gttagctggg cgctggccga gcaccgcggg tccacgcgcg tgcggctggc
     3361 cgctgccgtc gagcgcgccg cgcgggtcga ccgggcgctg ctcgcgctcg gcggtcggcg
     3421 ctggctgcgc gcgcggttcg agtcgacgtt ggccgggctc gtggaggagt tcgccgcgcg
     3481 agcgccggct cagccggccg cccactcctc gaccgtgccg taggcgcaac gcaggcacgc
     3541 ggcgaccgag tcccagtcga cgcggtccgg cgtgtcggtc ggctggtggt agttcgtgaa
     3601 gcggttgccc gtggcggaga ggagcgagat cgtgggcaga ccggcgtgca cggccagcag
     3661 cgggtcggtc cacccgccga gctgcacgcg ccgcggcggc tcgatccccg cccgcgcggc
     3721 gccgcgatcc gcgatgtcca ggtcctcgtc ccggtagcgc cagggccgga tcgagccctc
     3781 ggcgcgcgcg acgatcggct cgcccgcgcc gagcgtgtcg agtcccagca ccagcgtgga
     3841 gctcggatcg agccgcccgc cgtcgccgcg caaccagtgg gccatcccgc ccatgccgga
     3901 ctcctcgcaa cccgtgagca cggcgatcac gtcggtgccc ggcagcggct cggccgcgaa
     3961 gcgctcgacc atcgcgagca ccgcggccac gcccgtggcg ttgtcgctcg cgccgggcac
     4021 agatgggctg cgagcaacgt cgagcgccat ggccagcgcg acggccaggc tcgccccgcc
     4081 ggcggcgcga agggcgcggg ctcgcagcag tgagcccgcg gccaccgccg cgaacgccag
     4141 cttcggcgcg gtcgccggcg agtccatcac gtccggctcc ggctcgcggc cggtcagctc
     4201 gcgcagccgg tcgcccgtcc cgatcccgat cagctccggg ttgcgccagc cgatcccggt
     4261 gtgcgcggtg tcgtggtgtg ccacgagcac gagcgtgcgg ctggactctc ccgcggccgg
     4321 cacgcgcgcc accgcgttgg cgccttcccc ggcgggcacg agccggcgca gccactgcga
     4381 gcggccgccg aagtccagct cgaacgacac gagcgacgcg agcgccaccg cggcaccggc
     4441 aacaccgccg gcccgcgcgg cggcgatacc ggccgcgagg tgcgggacgc tgcgctcggc
     4501 gatcgtccgc tggtagcgga agcgctcgac ccgcacgtcg cgcgccccgg cctccgccag
     4561 ccgtctcgcc ccccactccg ccgccgcgac atcgcccggc gaggccgacc cgcgctccat
     4621 cgccgccagc gcctccacgt cggcgcggat gcggtcgagc gcggcgttca gctccggtgc
     4681 cagtcgaccg gccccgcgac gatcacatgc ccggacagcg ggcgcccgag cacccgctgc
     4741 gcctcgcgcg cgcagtcgac gagcgcctcg aggtcgatgc ccgtcgggtg ccccatctcg
     4801 tgcagcatcg agaccaggtc ctccgtggcg atgttgccgg tcgccccgcg cggtaccggg
     4861 cagccgccga gctcgccgaa cgacgactcg aacgagcgga tccccgactc gatcgccgcc
     4921 agcacgttcg cgaggccctg gccgcgggtg ttgtggaagt gcgcggtcag ctccacctca
     4981 ggcagccgct cgcgcgccgc cgccatgaac tcgcgcacct gcgccgggtt ggccatgccg
     5041 gtcgtgtcgc cgaacgcgac ctctgcgcag ccggccgccg ccaggcgctg cgcgatctcg
     5101 aacacccgct cgggcgcgac ctcgccctcg tacgggcagc cgaacgccac cgagatcacg
     5161 ccctcgcagc gcagcccctc cacccgcgcg cggccgaaca cgcgctcgag cccggcgagc
     5221 gactcctcga ccgagcggtt cacgttcttg tggttgtgcg tctcggtggc cgagaggaag
     5281 ccgttgacct cgtggaagcg ctcgcgcagg ggcagggcgt tgtcgaggcc cttctcgtta
     5341 gggatcagga ccgagaccga cacgtcgtcg gggatgtccg cgcgggtgag cacctcggcc
     5401 gcgtcgccga gctgcgggat cacgtccggc cggacgaagc tcgtcacctc gaggcgccgc
     5461 agaccggtgc gcgcgagcat ctcgacgagc cggaccttgt cgtcggtcgc gatgacctcg
     5521 ggctcgttct ggaacccgtc gcgcgggccg acctcgcgga cctggatcgg gtccgccacg
     5581 gctagatgat cggctccagg tccgcgtgcc gctgggaccg cgaaaatcga gcggggccag
     5641 gacgcagggc gaaggtcatg cagacgtgca tgaccgagcc cgaggacgcc gccccgcgac
     5701 gatttgcagc ggaatccagc ggtgcgggga tctggagtcg gtcatctaga tcctgcccat
     5761 gcccccgctg acgccgagcg tctcgccggt gacgtaggac gcgtcgtccg acgcgaggaa
     5821 cgcgatcgtc gcggccacct cgtcaggctg gccgagccgg ccgagcaccg tcttagagac
     5881 catcccgttg atcagccgct cgccgaggtc gccgaggtgc cgcgcggcca tcaggagcgg
     5941 cgtctcgatt ggcccgggcg cgaccgcgtt gcaggtcacg tcgtagcggg cggcctccgc
     6001 ggcgatcgcc ttcgtgaagc cgatcacgcc gcccttggcg cccgagtaga ccgccgagcc
     6061 gggcgagccg acgcggcccg cctccgaagc cacgttcacg atccgcccgc gcttgcgctc
     6121 ctgcatgccg ggcagcaccg cgtgggtgca ggcgatcacg ccgaccaggt tcacgccgag
     6181 cacgcgttgc cacatgtcct ggtcggtgtt gacgaagaag ccgaactcgt cgtacccggc
     6241 gttgttgacg agcacgtcga tcggccccag gtccgactcg gcggcggcga tcgcggcgcg
     6301 caccgactcg tcctcagcaa cgttcagctc gtagggctgg ccgctgacct cctcggcgat
     6361 ggtccgggcg ccgtcgaggt tcaggtcggt gacggccacg cgcgcgccct cggccgccag
     6421 cctgcggcag gtggcggcgc cgatgccgct ggcgccaccg gtcacgagcg cgatgcgtcc
     6481 ctcgagtcgc acggccgcgg agcctaccga tagcctcgcc gcgcatggag gacacggcct
     6541 cagtcccgat cgtcggcggc accggagcgc tcggcttcgg cctcgcgctg cgctgggcgc
     6601 gcgccggggt gccggtcgtg atcggctcgc gcagcgccga gcgcgcggag gaggcggcgg
     6661 ggcggctgcg cgagcgcgtg ccggacgcgc aggccgaggg cctcgagaac gccgaggccg
     6721 tccgccgcgg gccgatcgtg ttcctcacgg tcccgttccg caaccagtcg gagaacctga
     6781 cgaacctgaa ggaggcgctc gagccggggc agatcctggt cgacgccacg gtgccgctcg
     6841 ccgcggccgt ctcgggcaag gccacgcgcc tgctcggggt gccgcagggg tccgccggcc
     6901 agcaggctca ggagatggcc cccgaaggcg tgcgggtggt gagcgcgctg cacacggtga
     6961 gcgcggcgac gctgggcgag ctcgacgtgg agctcgacga ggacatcccg atcgccggcg
     7021 acaagcgcga cgacaagcgc cgggtggcgg agctgatcgc gcgcatcccg gggctgcggc
     7081 ccgtcaacgc ggggctgctc gagtccgctc gcctgatcga gggactcacc ccgctgctga
     7141 tctcggtcaa cgcgcgctac aagacgcacg ccggggtgaa gttcaccggc ctccccgacg
     7201 agctttggta gtcgtcctcg ccggcggcac cggcggcgcg aagctcgcac gcgggatgct
     7261 cgacgccgca gatgcgctga cggtggtggc caatacggcc gacgacaccg aggtctacgg
     7321 cgtgcacgtc tcgcccgacc ccgacctcgt cacctactgg ctcgccgacg cgatcgacgc
     7381 gcgcggctac gggatcgacg gcgacacgtg ggaggtgatg ggcgcgctcg aggccgccgg
     7441 gcgcgagacg tggttccgcc tcggtgaccg cgacctggcg atgtgcctga tccgcacgga
     7501 gctgctggct gctggcgagc ggctcaccgc ggcgcacgac gcggtcgtgc gcgccatggg
     7561 ggtgcgcgcg cgggtgctgc cgatgagcga cgagccggtg cggacgttcg tggggagcgg
     7621 cgggcgcgcg cgggcgttcc aggagttcat gatcgtggac cgcgccgcgg gcccgatcga
     7681 gtcggtggag ctgcgcgggc tcgagtcggc gcggccgggg cccgaggtgc tcgaggcgat
     7741 cgcgtccgcc gaggcgatcg tgatcggccc gtccaacccg gtgatctcga tcgggccgat
     7801 cctcgcgctg ccggggatgc gcgccgcgct gcgggcggcg tccgcgccgg tcgtcgcggt
     7861 cagcccgttc gtggagggac acgcggtgaa ggggccgacc gagccgttca tggagcactt
     7921 cgagctggag ctcggagccg cgggcatggc cgccgcgtac ggggacgtgc tcgacgggat
     7981 cgtggccgac gaggggttcg gcggggcacc cgtacccgtc ctcgtgaccg acacgctgat
     8041 gggcgacgcc accgcgcggc gccgcgtggc cacggagacc ctggagtttg cgaggagcct
     8101 gtccggcggg agagcgtccg ccgggccgga tcggcggtag agtcgccgtg ccgtacggag
     8161 tccgcccttg cgcacgctcg cgatagtccc ggtcaagagc ttcgacgagg ccaagcagcg
     8221 gctggctgct tcgctcgccc gaggctcacg cagctcgctc gcccaggcga tgttctccga
     8281 cgtgctcgcc gcgctcgacc gcgcgcgctc ggtcgaggcg gtggcggtgg tgacggccga
     8341 ccaggccgcc cagcagctcg cccacgggcg cgcgctggtg ctgcacgaca gcgcgcaggc
     8401 cggacagtcc gacgccgtcg agatcgggat ccgccacgcg gtcgcggcgg gctacccgcg
     8461 cgtgctgctc gtgccgggcg acacgccgct gatggacccc gccgaggtcg acgccctgct
     8521 cgacgcctcc gagcgcgagg gcctcggcgc cgcgatcgtg gccgaccgcc acgggaccgg
     8581 caccaacgca ctcgtgctca cgccgcccgg cgcaatcgcc cccgcgttcg ggccggggag
     8641 cctcgagcgc cacgtgcgcc tggccgagga ggcgggcgtg gcccaccggg tggcggcggt
     8701 cccgtcgctc gagcacgacg tggacacccc ggaggacctc gccgcgctgc gcagcgtgct
     8761 gagcggcgtg cgcgggcgcg gccagcggac ctggggcgcg ctccaccaga tcgaccacag
     8821 ccgcgcgact cccgtcgagg tccgggcctg agcgtccgga tagcggcgct ggcggacctg
     8881 cccgaggtcc gtcccggcga cgacctcgga gcgctgatcg ccgacgccgc gccggccgac
     8941 ctcgcgagcg acgacgtgct ggtggtcgcg cacaaggtcg tctcgaaggc cgagggccgg
     9001 ctgcgccggc tcgaagaggt cgagccgggc gagcgggcgg tcgagctcgc ggctccgtac
     9061 gggaaggacc cgcgcgccgt gcaggtcgtg ctcgacgagt cggccgagct actgcgggcc
     9121 gagcgcggcg tgatcgtctc ggtcacgcgt cacggcttcg tatgcgcgaa cgccggggtg
     9181 gatgcctcga acgcgccgga gggaacgctc gtgatgctcc ccctcgaccc cgactcgtcc
     9241 gcgcggcggc tgcgcgcgcg gctgggcgag ctgcgcggcg tgcgcccggc ggtcgtggtc
     9301 accgacagct tcggccgcgc gtggcgcacc ggccagaccg acgtggccat cggcgccgcc
     9361 gggctcgtgg cggtggacga ctggcgcggg cgccgcgact ccgtcggccg cgagctgaac
     9421 gccaccgcca tcgcggtggc cgactccatc gcgggcgccg ccgacctcgc ccgcacgaag
     9481 gactcccgcc gcccggcggt gctggtgagg ggcctgggcc acctggtcac cgaggacgac
     9541 ggcccgggcg ccgcgccgct ccgccgcgaa ccggaccagg acctcttccg ccgctgagtg
     9601 caaaagcggg actatttgtc ccgctttcgc acacacccga agtcacttgc gtccctatac
     9661 gggacgcttt tgacttcacc ctgggtcaag ccctcgcgtc ggctgccgaa aaccgtcaca
     9721 tggcacggat gcccgcagcg ttcacggctg ccctgatcgc cgcccttctc ggagccgcgc
     9781 cggcgagcgc gcagctcccg accgacctgc ccgccggctg gggcggcggc tcgagcgcgc
     9841 agcgcggcga cgaccgtggc cactggggcg gctgggacga tcagaacgag gacgagcccg
     9901 ccgacgagcc cgctccggcc gacgatcccg ctccggccga cgagcccgct ccgcccagcg
     9961 gctggatcga cggcggcgga ctccttccgg gcggcggtga cgagcccggc gccgagctgc
    10021 tcccgcccgg cggcgcgcgg ccgatcgtga aggggcggct cgccgcgctc ggcacgaacg
    10081 gcgccgcgta cgcgccgtcg aaggcgccgc gccaggtgaa gcttgcgatc tgggccgcca
    10141 accagctcag gcagaagccc tacaagtggg gcggcgggca cgcgcgctgg agcgactcgg
    10201 gctacgactg ctccggctcg gtctcgttcg ccctgcacgc cgccggcctc ctcggcggcc
    10261 cgcaaacctc cggcacgctc acgcgctacg gcaagggcgg cctcggcaag tggatctcga
    10321 tctacgcgaa caaaggccac gtgttcatgg tcgtggcggg cctccgcttc gacacctcgc
    10381 cccacggcag cggcgagagc ggcccgcgct ggcggccgac cggccgcccc gccggcggct
    10441 tcaaggtccg ccacccggcg gggctgtagc cgcgcggagc cggcgccccc tggggcccgg
    10501 cgggtccgcc ggcgcgcggc gcggccccct ggggcccggc gggtccgtcg gcgcgcggcg
    10561 cccctcgggg gtcggcgcgc aggcgcgccc cctcggtcgg cgcggcggct agcgccgcac
    10621 cgtgcagcgc gcgtgcttca cgcgcacgag cggcttcgcc gccccgacgc gcaccttcgc
    10681 caccgcgcgg aagcgctgcg agcgcgccga cggccgtagc cgcaccttca ccgagaacgg
    10741 cgccttcgaa tcctccacct tcctcccccg catggcgaac ctcacacggg aggcggaccc
    10801 ggcatcggac ccggtggtgt aggccacgac cccccgcgag gagcaccgca gcctgagctt
    10861 cacgaccgcg gctcgcggcg gcggaggagc cgtgaccggc ggcgcggccg gcgccggggc
    10921 catgtaccgc gcgaagcggt cgcgcagcac gtcgaacgcc ggcttgggcg tgtagtcgtc
    10981 gcgcgtgatc ccgtactgct gctgcgggtt cgggttgtcg gactcggcgt cgcgcaggtc
    11041 gaaccagaag tggtcggtga cgttgtagac ccgggcgtag tcgctcaccg cgtcgagcat
    11101 cgacgtggcg gccaccgcct ggtcctcgta cgggcggtcc gggctggtcg ggtagccgtt
    11161 ctcctcgatg tgaatcggaa cggtgtcggg gatcccggcc agcggcatgc tgcactttcg
    11221 cagcgtgctc atcgcgtgga ccatgaagtc cccggtctgc gacggctcga ggcgcggcgg
    11281 gacgaagaac cccgggtagg cgtcgagccc gacccagtcg agcgacttca cgaagcgctc
    11341 accgcccgcg ctgccgaggt actcccagaa gctgcgctcg gtctgggggt ccatgcggta
    11401 gacccagttg aagccgatct gcacctgcga gtagccgagc gagcgcgcct cgtccttggc
    11461 ggcgatcaca ccctggatca gcgcgtcgcg cgcccccttg taagccccgt cggaggagtc
    11521 cggcgagacc tcgaagttca cctcgttcgt gatctgaaag cccttgacga gcgggttcga
    11581 cccgaagcga cggaccaccc cgcgcacgaa cgcggcaaag cccgccacgt cgtcgtcgcg
    11641 gcggtagcgg atcacgatct cggtgccgta accgcggctc gtgtagaggt cgacgagccg
    11701 gcggtgggtg cgctcctcct tctcctgctc ctcgtcggtg tagtacgccc ggtagaggtg
    11761 cacggcgaag ggcccgccgg tcggcttgag ctgggccagc gccgcgaaga tcttgtcctg
    11821 gttctcgcgt cgtgagctcg acgggatccc accgagctgc ccggccggcc cgcccgggta
    11881 gatcccgtag cggagctggc cgcccggctg cggcggcggc gccgtggccg ggaggtcagc
    11941 ggtgcacggg gtctgtccgg ccgccggacc ggcagccgcg agcgcgacga gcgccgttgc
    12001 cacggctgct gcgagacccc tccccacgcg ggcgagggta gtccgctacc cccgcgactg
    12061 cgccagcggg tccggagcgc cgagctccgt gacctccttg ccctggtacg gcctccccgg
    12121 gtgcccgccg agcgccagca ggcgcagcgg agcatcgccg gtcgtgatcg cgcgccgcgc
    12181 cgccggacct acgcgcacca tcatccccgg ctcgagcacg tggcgatcgc cgtccaccac
    12241 cagctcgccg cggccgctga gtggcacgaa gacctcctcc tgcccctcct cggcgtgatc
    12301 gtgctcgggg tgctggtcgg agttcggcgg gaagtcgagc acctgcatcc cgaacgaagt
    12361 cacgcccagc tcggcccgtg cccgcttgaa gccgccgccg tagacggcct ccatctcgtc
    12421 gatccgcttc accgtgtagt cgctcatgct cgcccctgac tcgacccccc ggcgcggtgc
    12481 caaggggatt tgcataccgt cagtaggttt gcagaacccg gtacagcgtg tcgcgctgga
    12541 ccggcgcgcg gcccgcggcg cgagcggcgg cgatcaggtc gtcggggtcg accatcgtgc
    12601 cgtggtagga gccggccatc cggctgatcg actcctccat tagcgtcccg cccaggtcgt
    12661 tcacccccca gcgcagcgac tccgtggccg cgtcgagacc catcttcacc cagctcgcct
    12721 ggaggttcgg cacggtcctg ccgagcgcca gtcgaaacgc cgccgtgtgc ttgaggttct
    12781 cctcgcgcga tatctcctcg atcccgtgcg tcctccccag gagcgtgtgg aacgggatga
    12841 acgagagcgg cacgaactcc gtgaagccgc cggtccgctc ctggagcccg cgtacgacgc
    12901 gcatgtgctc ggccagctcc cacggctcct cgacgtggcc gaacatcacc gtggcgctcg
    12961 agcgcagacc gctcgcgtgc gatgcctcga tgatctccac ccagcggccg acgggcagct
    13021 tgttcgggga gatccgctcg cgcacgccgt cgtcgagcac ctcggcggct gtgcccggag
    13081 tggacccgag gccggcggcg cgcaggcgct cgaacacctc ccgcggcggc agccccgaca
    13141 cctcgcacat gcggtcgacc tccatcgccg agtacgcgtg caggtggatc tcgggcgcca
    13201 gctccttcgc gtagcgcagc cagtcctcgt aggtctcgag cgtccattcc gggtggatgc
    13261 cggactggat gcagatctcg gtggcgccgt agtcgagcgc ctcgtccacc ctgcgggcga
    13321 actcctcgcg gtcgtgctcg taggcgtcgg gcgagcggcg cccctggccg aagccgcaga
    13381 aggcgcagcc gaccacgcag acgttcgaca ggttgatgtt gcggttgacg acgaacgtga
    13441 cctcgtcgcc ggccagctcg gcccgcagct cgtccgccgc ctggcgcatg tcctcgatcg
    13501 cctcgggacg cgtctcggcg aacagcgccg tgagctcctc ggggctcagc tcgacgccgt
    13561 cgcgcccgcg cgcgatcgcg cccgggacga ggtcgcgccg gatcgcgcgc tcctcgcgcc
    13621 ggccggaccc gcgccgcggg atgaagctcc agtagcgcga cttgatcacg tcgagcacgc
    13681 cctgctcgat ccactccggc gtcatgtact gcgggtagac gcagagccgc tcggtgagcg
    13741 cgtagccgcg cggcgccagc tccttgcgca tccggtgcgg ggacgggaac gggtgctccg
    13801 gcgagatgtg gtcgccgttc gcggagaggc cgcccaggtc ggtcgcgccg tcctcgacca
    13861 gcggcagcca ccagtccgac aggttcggcg ggatctgaac gcccacgtcc ggcatcaggc
    13921 gtttggcctc gcggaccagc tcgcgcatgt cgtcgagctc gaccggagtg gcccacggcg
    13981 gcatggcggc cggctcgccg tgcgcgccgg agcgctgcgc ctcgtcggcg atcgcggccg
    14041 gctcggcgcc gtagtagcgc gggtgcggga cgaagttctg gaggatcagc tcctggatgt
    14101 gaccgtgctc ggcgtgcacc gcggccagcg cctcgagggc ggccacccgc tcctcgcgcg
    14161 actccccgat cccgaccagg atgccgctcg taaacgggat gcgcagctcg cccgccgcgc
    14221 ggatgcagtc gagccgcagc tcggggtgct tggtcggcga gccctggtgg acgaccaggt
    14281 ccgggttcac cgactcgagc atcagcccct gcgaggccgt cacctcgcgc agccgcgcca
    14341 aatcctccct gctcgccacg ccgatgttcg tgtgcggaag cagcccgcgc tcgagcccgc
    14401 gctcgcaggc ccaggccaca tacgaggtga agtcctcgtg gccgtactcg gcgagcttcg
    14461 cggccacctg ggggttgacg tccggcgcct cgcccgtgag cacgagcagc tccttgaccc
    14521 cgcgccgggc cgcgtcgtcg agcagcttct cgacctcgtc aggcgcgtac aggtgggcgc
    14581 ggtgcgtcgc gaacgcgcag tacttgcaat agcaccggca cgtccgcgac agcgacagcg
    14641 tgaagttccg cgagaaggtg acccggcgcc tcatcggagc cggagtctac ggacgcgggt
    14701 gggcggcctc ggggaccgcc cgggtcagcc gaccggttcc gctcccccgg tccccttttt
    14761 cccgtgcgtc accatcaaat acgagtagag gaacaccgcg agcagggccc cgagcacggg
    14821 cgccaggacg tagaggagca gccacttgcc ctcgtcgccg aacgacttcg ccacgatcgc
    14881 cgggccgagc gcgcgggccg ggttgaggcc ggctccggtc atcggggcga tgatcatcac
    14941 cgcgaagccc agcgtgccac cgatcacgag cccggaccag tccttgaccg cgctcgggtt
    15001 gaccgccact cccatgatcg cccagacgag gaagaacgtt cccagcgcct cgagccccat
    15061 gccctggagc gtgttgtcga gccggtccgc gttgaacccg acggcgccga agttctgccc
    15121 gttctggtcg ccgatctcgt tgtagaggcc cttgaccgcc agcgcgccca gcgtgccgcc
    15181 ggcgagctgc atgagcacgt agatggccga gtcgatcggc ttgatctgcc gcaccgcggt
    15241 catcgcgacc gtgacggccg ggttgaagtg cgcccccgac atgacggcca tcgtctggat
    15301 caggaagaac aggaccagca cgtggaccag gccgacgacc gcgaagtcct ggaagaacga
    15361 ctgcgccggc gcaccggtgg gactggggtc gggcgggccc gggacgaaca gcaccacggc
    15421 ggcgcagatg gcgaacacga gcgcgaacgt tccgatgaac tcggccacgt aggcggccgg
    15481 cccgcggtcc gggatccgca ccgttgcggc gttcaccacg ctgccctcgg ctgcgtttcc
    15541 tgtctctgcc atctttggtc aggcctccct ggttcgggtc aagttggccc ggggccaccc
    15601 ctcggaccgc gaaagcgggc gcagtctagc taccggagcg gtcgccacga gccttggcgg
    15661 gcggcgccga gcgtgcgggg ccgccgctca cccgcccatg ccgcgcgcga tgcggcgcac
    15721 gacgcccggc gcgagtgtcc gaagtatgac cgggatcagc cacgggcgcg ggacgtagcg
    15781 ctccggcttc ccgcccgggc cggcgtccgc gatcgcctcc gccaccctgt ccggcgtcga
    15841 cacgacccag cgcgtggccg ccttcccgac gagctgctcc tgcgggaacc cctcggtggc
    15901 cacgaagccc gggaacacga cgcccacgtg cacgccgtga gcctcctcct cgtagcgcag
    15961 cgactcgctc cacccggtga ggccgaactt ggacgctgcg tactggccgc cgcgcggata
    16021 cgggatgcgc gccgcgaccg acgcgacgtt gacgatcgag ctcggcgccg agctcctgag
    16081 cacgggcagg agcgcctcgg tcagccggat cggcgcgtgc aggttcacct cgagcacccg
    16141 ctccacgttc gcccagccgc cgtcgtcgtc gccgaagtac gcgcgccagt tcgcgcccgc
    16201 gttgttcacg agcaggtgga gctgcccgcc gaaccgctcc tccacggcct cgcgcacccg
    16261 cgccggcgcg tcgtcgtcgg tgaggtccag cgcgagcacg ctcgcgtccg gcagcgtccc
    16321 cgccagcccc tcgagccggt cggcccggcg cgccacgagc agcagcttcc agccctcgtc
    16381 ccccagccgc cgcgcggtcg cctcgccgat cccgctcgac gcgccggtga tcacggctgt
    16441 gcggctcacg cggccacccg ctcgaccagc gtcgccatct cgcgcgccat ccgcgagcgc
    16501 ggcacgacca ggtcgaaccc ggccgcctcg gcgcgctgct tggtgtccac gtccgtgtgg
    16561 ccatagaagc cgagcttgcc cacgccggcc agcgcgccga gcgcgggccc gtcgaaggcc
    16621 tccgcgttga ggtccacaac cagcacgtcc gcctcggcgg ccgccgcctc gcgcgcggcc
    16681 ggctcgcggt cgtagcgcgt gacctcgtgc cccacggcgg cgagcccgcc ctcgatcctc
    16741 gacccgaaca gcaggtcggg gcacagcagc gccacccgcg ccatcactgc gcccggcggc
    16801 ggcggtgctc ctggcgcacc gactcgggcc gcccccacat cggagtgacc tcgacgcgac
    16861 ccacccgcgc gtcgcgcatc gcgagctcgc cctcgatccc ctcgccgtcg agcacggcca
    16921 gcgtgccgtg caccgtctgc ggcgcggcgg cgtggccgaa ctcgcgcatc gagtgcagcc
    16981 gccagtgcca gtcgtgcttg gagtacggct gcgcgttgac ctgcacgagc agcgaggcgg
    17041 cctcgacgta gcgctcctcg tcgaagatcc ggaggtcgag ctcgaggtgg gtccagtccg
    17101 gagggaggga cccgaggatg cggtcgaagt cgtccgccag cggcatgcgg cgaacactac
    17161 cctcgaccag gtgatcctcg cctccgagta cgagcttctg cgcgaggacg cgggcgccgt
    17221 cgagcgccgc cgcggcaagc tcctgctcga gggcgccgag acggccgcct tcctccaggg
    17281 ccaggtcacc aacgacgtgg aggggctcgc gccggggacc ggctgctacg cgctgctgct
    17341 gggccacaag ggcaaggtcc ggttcgacat gcgcatcctg cgcgggccgg actggatctg
    17401 gatcgactgc gccaagggtg cactgccggt gctccagcac acgatccgca cctacgggat
    17461 cgggcgcgac atgcgctcgc gcgacgtgac cgaggagcgc tcggtcgtgt ccgtgatcgg
    17521 gccgcgggcg cgcgccgcgc tcggtgtcga cgtacccgag gaggagcacg cgttcgtgga
    17581 cgacggcgga gtgctccacg tggcgaccga cgtcggtgtg gacgtgatcg gcccgcccga
    17641 ggcgctcgcc cccgagatcc ccgcggtctc cgaggaggtt gccgaggtgc ttcgcatcga
    17701 gtcgggccgg ccccgcttcg ggctcgactt cgacggcgac acgatcccgc aggaggccgg
    17761 cctcaacgag cgcgccgtga gcttcacgaa gggttgctac gtcggccagg agacggtcgc
    17821 gcggctgcac tacaagggca agccgaaccg ccacctgcgc gggctgcggc tgtccgagcc
    17881 cgccgagcgc ggcgtcgagc tgcgcctcgg cgagcgcgtg gtgggcgcgc tcggctcggc
    17941 cgcggtgtcg cccgtgcacg gcccgatcgc gctggcgctg gtgcgccgcg aggccgagcc
    18001 gggtgccgtg ctcgaggtcg gggcggacgg cgtcaccgcg caggtggttg agctcccgtt
    18061 ccgaaagtcc gacgggtgac cggctcaagc cgcgcctttc gacatccgac gctgttcgta
    18121 acaggagcgc gcttgcccct tcgaccgatc ctcaccctga tcgcctgcct ggtctgcgcc
    18181 gcccccgccg cggcggatgc gcccgtgcgc gccagcagcg ccggcgccca gagcgaggcg
    18241 cgcgcgacgc tggatagggc tcaggccctg ctcgacggcc gcggcgtgga gagcggcaag
    18301 gagctgaccc cggtgctgaa ggacctgtcc ctgcggctga aggcgctccc cgagggtgac
    18361 cgccgcgagg cgcgcgccat cctcgcccgt cccaccgtcg ggcaggccca ggccggcgag
    18421 gaggcctacc ggcccggctc gaccgagcac cgcatctgca cggcgcactt ctgcatccac
    18481 tgggtcacga gccccgaccc cgaggacccc gacccgccgc cgctcgactc gaacgacggc
    18541 gacgcgatcc ccgactacgt gcagacgatg agccaggtgt tcgagaacgt cttcgccgtc
    18601 gagaacatcg gcatgggctg gcgcgagccg acgcccgacg acacccgcgg cggcgacttc
    18661 aacaaggtcg acgtctacat caagcagctc ggcgaccagg gaatcttcgg ctacgcgacg
    18721 cccgatcccg gccaggagtc gaactcccag gcggcctacc tcgtgatgga cgacgacttc
    18781 acgcccgccg agttcccccg ctacagcgag ccgctgccgc cgatgcaggt gaccgccgcg
    18841 cacgagtaca accacgtgct ccagttcggc tacgacgtgc tccaggactc gtggatgttc
    18901 gagtcgaccg cggtctggat ggaggacaag gtctacgacg acatcaacga ctacgtctcg
    18961 tacctcgggc cgtggacaca gctcacgcag gtcccgctca ccgcgttcaa cccgggcgac
    19021 ctcgcggacc cgatgaacgt caaggtctac ggcgacgcgg tctggaaccg ctggatcgac
    19081 gagcactacg gggccgacac cgtgcgcggc gcgtgggagc gctcgctcga gacgcggccg
    19141 ctctcgttcg cccccgacgc ctacgacgcg tcgctcctcg cgaagggcac caccttcttc
    19201 gactcgttca cgcgcttcgc cgccgatacc gccgagtggc gctccgcggc cgggccgttc
    19261 gaggagggcc cgacctggcc cgacgtgcag cgcgcgagca agaccaacct cgcgcccggc
    19321 ggccccgggg tcgagggcag gctcgaccac acgacgttcg cgctcctcaa cgtcacgccc
    19381 accgccgacg agcggatcaa gctgatcggg tcgctcccgc gcggcgtgcg cggcgcgttc
    19441 gcgctggtcg gccgcgaggg cccgatcgac agcggtacgc cggtcgtcga gctcaagcgg
    19501 ctcacctccg gcggccagac gagcgtcgag ctcgtgaacc cgggtcgttt cgagcgcatc
    19561 accgcggtgc tgatcaacgg cgacgtgacc cagaacgggt tctcccagtt cacgggcgac
    19621 tgggcctact cgaaggacaa ccagccggtg ctcgcacacg tgtcgagcga ctacacgcca
    19681 ccgcgcgtgc gcaagcgctc gcccgcatcc ggcgcgaagg tggcgcggtc ggcaccggtc
    19741 gtcgtcacgt tctcggagcc gatgcagaac atcagcacga agacgctcga cctgatcggc
    19801 cccggcggcc ggcacgtctc cgcgcgggtc agctacgacg cggcgaagcg gcgggcgcgc
    19861 ctggtcccca agcgcaagct cgatccgcgc cgccgttaca cggtgaagct cggcagcacc
    19921 gtggtcgacc tgggcgacaa tcccctcccc gccaaggacc ggagctggaa gttctcgacc
    19981 aggagcaagt aggcgcgacc gtcctcggtg tagcaggcgt cattcggggt acgatgcgac
    20041 aactcgcccc tccggaccag gagcgagggc cgcgcatgac gacctacatc tcggaccaga
    20101 gggcacggag gctcgccgaa ctcgacgcac gtgagcgtca ggcgtgggag acgtacagcg
    20161 agagcgttcg cgggctcgag ggccgggact acgaggagac agaggatcag tcgtgggacc
    20221 gcttgcagat gaagctcagg gagctcgagg acgagcgtca gctcgtcgcg ggcgactgag
    20281 ctccgacagc ccgctcttag gcggatcgct cgacacgggc gccggggccg aggagccccg
    20341 gccgaccgcg gtcgactacg acccgcgctc ggacctctac gaactgccgc ggccgcaggt
    20401 gcgcgacacc gctctcgacg aggtggccga cccgcgcctg ctgcgcgagc tgatcccggc
    20461 cgcggagccg ggccgcgacc tcgacgactg gggccgctcc gagcgggtct tctcgctcat
    20521 ggagccggtg ctgaacttct actaccgcta ctggttccgg gtcgagcagg aggggatcga
    20581 gcacgtgccg gacgagggcg gcgctctgct cgtgtcgaac cactccggcg cgctgccgcc
    20641 cgacgcgccg atgatcatgc agacgctgcg caacgagcat ccgacgtcgc gaccgctcta
    20701 catgctcggc gagaactggt tcaagggcta tccgggcgtg ggaatgctcg cgaacaagct
    20761 cggcctggtg tccgcgcacc cggccaacgc ccagcgcctg ctcggcgagg aggggcggct
    20821 cgtgctcgtg ttccccgagg gtcagaaggg gacgcgcaag ctctactggc agcgctaccg
    20881 gctgcgccgc ttcggccgcg gcgggttcgt gaagaccgcg ctgcgcgccg gcgtgcccgt
    20941 tgttcccgtt gcagtggtcg gggccgagga ggcgatgccg atcttcgccc atgtgcctct
    21001 gcttcagcgc ctcacgggcc tcgtctactt tccggtcagc cacgcgttcc cgcacctggg
    21061 cctcgcggcc ggccttctct acatgcccgc caagttcaag atccgcttcc tggagcccgt
    21121 gcacttcgac ggctacggag ccgaggacgc ggacgacctc gagctggtcc agtcgctcgc
    21181 ggagggcatc cgcgagcgga tccagcgcga ggtcgatcag atgatctctg ctcgtacgtc
    21241 cgtgtggttc gggtagggtt cggcaggggg atggggagga tgaccagagt cggacggaac
    21301 cggaccgcgg cgctcctcgc gggggcgctc agcctgctcg taatgagcgg gtgcggagac
    21361 gacgacgact tcgcgaacga cccgcgcccg ccgtcgcccg tccagctcag tggcgtgatc
    21421 acgaactcgg acgtgtcggt ggagccgcgc aagatcggcg ccgggccgat catcctgatc
    21481 gtctcgaacc agaccgagca gtcgcacacg gtcacgctcg agggtccgag cggcagcgag
    21541 gaggtcgggc cgatcaaccc cctcgacacg ggtcgcatcc agcacaacct cgagcagggc
    21601 tcgtaccaga tcaaggccgg ctcggacaag gccgtggacg aggagatcca gcccgccgtg
    21661 ctgagcgtcg ggccgaagcg cgaatcctcg tcgaactccg tcctgctgcc gtaggcggcg
    21721 ggccgctgcc gtaggcgggg ctccgctgcc gtaggcggcg ctccgctgcc gtaggcgggg
    21781 ctccgctgcc gtagacgggg ctctgctgcc gtaggcggcg ctcctctgcc gtaggcggcg
    21841 ggccgccgca ccgccctggc gaccgggcgc cggccccggc tccactgggc catcttttgg
    21901 tcgtatatga cacctaactt cgcccagtcg ctcggggtca ggcggtgtcg ggccgcgctc
    21961 aggccactgg gcgaactttg ccccgtatat cggggctaac ttcgcccagt tggctcaggc
    22021 gatgagcgac cggggagatt cctccccacg cggcgctcga gtgcgcgccg ctgcgctggc
    22081 gaagctgccc ctccgccgag cgacgcgggc cggagcgcgt cgcgtcccgc cgcgtgcgct
    22141 agacgagctg ttcctggccc gggatgacgg agagggccgg agccggcgct cgcgatcggg
    22201 cgggttccgt cgcggggggc tcccacgccg ccgcgggctc cgcggcgcgc gcgggctccc
    22261 gcgccgtcgc cgcgccctcg gccgcgccgc catccaccgt cgcaccgcca tccaccgtcg
    22321 caccgccatc cgccgccgcg cccgcggccg cgccgccatc caccgtcgca ccgccatccg
    22381 ccgccgcgcc ctcggccgcg ccggcaccgc ccgccgccgc gccgccatcc gccgccgcac
    22441 cgcccgccgc cacaccgcca tccgccgccg cgccctcgtc tgcgcccgcc gccgcgagcg
    22501 tctcgatgtc gcgcaggtag tcgcggacgc ggttctccat ctcgtcgagc atcgagtagg
    22561 cgacctcgat gcccttcttc gagccggtga gctgtgactt gatcttgcgc gcgtcgtcga
    22621 caaccgcgag cgcgcgttcc accacgtcgc ggatcgcgct cgagtcgacc acgtcgtcgc
    22681 caccgcgagc catcagcacc cgcgcccgcg ccagcgagta ggccacctcg agcggcagcg
    22741 tgctgtcgcc ctcgggatcg aacgccacga gcagcttgtc gccgttgtac tcggtcagcg
    22801 gtcgcgtctt ggccggcagc cgctcctcgc tcggcacgac cagcaccgcg aagtcggcgt
    22861 cgcgctgcgc cagcgccgcg ttgagctgct gccacgcctt cggcttcgtg agcgccttgc
    22921 cgctcttggc ctcgaacacg atccggccgc gggccgggcc gttgcacgcg tcgagctcga
    22981 ccaccacgtc gcccaccttg ccggtcgagc ccgtgacgtc gcccaccgcg tcgcacgagt
    23041 cgtcgcgcgc ggcggcgatc cgctcgagcg cctcggccac ggcctcctcg aacgtgcgcc
    23101 ccttcgcgct cccgcgctcg cgctcggcgt cgacctcctc ggccttctcc ttctcggcgt
    23161 gcaggctctg caactcgatc ttcagcgccg tgagctgctc gttcagcgtg cgcaggttgc
    23221 tgtcctggcg ctcgccggcc tcgcggagct ggcgcagcgt gccggccttg aactccgcga
    23281 gcgggttctg gccgtcagcc gacgagaact gcttcagcag gtcctcgcgc gaacgctgca
    23341 tcacctcgtt gatcacgtcc ttcacgcggt tctgcacagc cccggcgctg tcgtcggaga
    23401 agtggcgttc gagcgccttc gtgagctgtc cgctctcggg cccgaacacg gcgtccatct
    23461 gcttctcgag ctgctcgctc acctcccgcg ctcgctcggt gaacgcggcc tcgacctcac
    23521 gcgagacctt gtcgaactcg ctcttcacga agtcggcgtt cgcgccggcc tgctcgcggt
    23581 cgagcacccg cgcgcctatc tcgatcgcgt cggcgaccgc gcgcaccggg tcgtcccccg
    23641 cctcctcgcg ctcgcgcacg agccgtacag cgcactcgtc ctggatgacg aggccgtcga
    23701 cgacgagacg gtcgtcgccg agcgttcgga tggttgcggt gcgcagtggg gtagcggctt
    23761 ccatggcagc agaccacggt aagcggggtc ccggacggag tgacggatgc cacactggct
    23821 gagcggccaa gtgccatcgc cgctgcaata cttgcgttca tgcaagcaac cgggaagatc
    23881 gctaagcggg gcggcggcgc gacggacggc ggagctgcga cggacggcgg agccgcgaca
    23941 ggtggcggag ccgcgacagg cggcggggcc gcgaccgcga cacccgccac cctcaccgag
    24001 gacctcgcca ccttcgtcaa gtacctcatg cacagccacg gcgaggactt cttcaaggcc
    24061 gtcggcgagc tcgacctctc gttcacccag gtgcgcatcc tcggcgtcct cgcgcgggac
    24121 gtcgagcgcg cgtcggtcaa ggagctggcg gacaacctcg gtctctccct ccccgccgtc
    24181 agccgctcca tagagggcct cgtccagcgc ggctacgtca cccgcgccga ggacagcgac
    24241 gaccgccgca tgaagcagat cgccgccacc ggcgacggca ccgcgctggt cgcgcggctg
    24301 atggagctcc gcgtggccag cgccgccgac ttcctcgcga cgctgagcgc ggaggagcgc
    24361 gccggcctcg ctgccgcgct cgagccgatc gtcgcgcgcg aggacgtcgc cgccctccgc
    24421 aggaaggtgc gctgagcgag atgccgcagc gcatgcgcct caacgaggac aaccgcaagt
    24481 ggtggacgct cgccgccatg tgcttcgccc tgttcatggt catgctcgac aacaccgtcg
    24541 tgaacgtcgc gctgccgtcg atccaggacg acctcgacgc gtcgctctcg gcgctcgagt
    24601 ggacggtcaa cgcgtacacg ctcacgttcg cggtcctgct cgtgaccggc ggccggctcg
    24661 gcgacatctt cggccgccgc cgcatgttcc tcttcggcgt tgtcgtcttc gcgatctcga
    24721 gcgcgacgat cgccctcgcc cccacccagg cctggctcgt cgcgagccga gcgttccagg
    24781 gcgtcggcgc cgccttcatg atgccgggga ccctctcgat catctcgaac acgttccccg
    24841 cgcacgagcg cgggcgcgcg atcgggacct gggcgggcgt ctccgcgctg gccctcgcgc
    24901 tcggcccggt ggtcggcggc gcgctcgccg agcacgtctc gtggcgcgcg atcttcgtcc
    24961 tcaacctccc ggtcgccgcg ggcgcggtcg ccgtcacgct gttcgccacg cgcgagtcct
    25021 acgaccgcac ggtctcgcgc gccgtcgact acgccggcat cgccacgatc tcggtcggcc
    25081 tcaccgcgct cgtgctggcg ctcgtcgagg gcaaccagtg gggctgggac tcgccggagg
    25141 tcctggcgct gttcggtacc gcggccgtcg ccttggtcgc gttcgtgtgg gtcgagctgc
    25201 acggccgcag cccgatggtg gagttctcct acttccgctc gcgctcgttc ctcggcgcca
    25261 acgcgatcgc gttcatcgtc tcgttcgcga tgctcgcgat gttcttcttc atggcgctgt
    25321 acatgcagaa catcctcggc tacagcccgc tggaggccgg gatccgcttc ctgccgagca
    25381 cggtcgtgat catcttcacc gcgccgatcg ccgggcgtct ggcggaccgc atcggaccgc
    25441 gcccgctgat ggccaccggc ctcgccgccg ccgccgtcgc gctctggatg cagtcgcggg
    25501 tgaccgtcga caccacctac tcggacctgc tcgcgtcgtt ctcgctgatg gggctcggca
    25561 tggggctgac gatgtcgccg atgtccaccg ccgcgatgaa ctccgtgagc gccgacaagg
    25621 cgggcgccgc ctccgggatc ctgtcgatgt tcaggatggt cggcggcacc ttcggcgtgg
    25681 ccgtgatcgg cgccctcttc cagagccttg cccacgaccg cgtgaccgac gcggcgcggg
    25741 ccgccgggct gccgcgctcg atggagaacg agctggtgga gaacgtgggg tccggccggg
    25801 tcgcggacat cgcgcaggga ctggacccgg tcgccgccga acggctcgcc gacggcatgc
    25861 gcgaggcgtt cgtcaacgcc ctcggcggcg ccctcaccct gtccgcggca gtggccgccg
    25921 ccggcgcggt gatcgcgctc gtgctcgtgg agccgaagcg cgcgcacagg cccgtggacg
    25981 tggcgcgcca gcccgaggcg gtcgagcccg ccggggcgca gtagccgggc ccggccgcga
    26041 ccgcgccgca cggctctgga cgcggcgccc gatacgatcc tgcggccctg aacccacctt
    26101 ccggaggtct gatccacagc atggccgttg acctgaagaa cgtgaccgac gtcgccaccg
    26161 agcccggcac gctgcccgag acgatggccg cctgggtcat ccgccaggag cgcgagggag
    26221 agccgaccga cgcgttccag ctcgaggaga tcgaggttcc cgagcccggc gcgctcgagg
    26281 tcgtcgtgcg cgtgatggcg gccggcgtga actacaacaa cgtgtgggcg gccctcggcc
    26341 agcccgtctc ggtcttccgc taccaccccg aggacgacca ccacatcggc gggtccgatg
    26401 cctccgggat cgtctggaag accggccccg gcgtcacgcg ctggaaggtc ggcgacgagg
    26461 tcatcatcca ctgcaatcag gcctcctacg aggaccccga ggtgcacggc ctcgacccga
    26521 tggcggcgcc gtcccagaag atctggggct acgagacctc ctggggctcg ttcgcgcagt
    26581 tcacgaaggt ccaggcccag cagctcatcc ccaagccgga gaacctgacc tgggaggagg
    26641 ccgcctccta cggcctcacg tacttcaccg cctaccggat gctcatcacc cggtgcaaca
    26701 tccaggccgg cgaccgcgtc ctgatctggg gcgccgccgg cggcctcggc gtgttcgcgg
    26761 tgcagctctg caacctcgcg ggcgcggtct cgtgcggcgt cgtgtcgtca gcggagaagg
    26821 gcgcgctgct cgagcagatc ggcatcacag gctggatcga ccgcaacgac cacgccggga
    26881 tgatgcgcaa gggcggcgag tcgcccgagg aggagaaggc ccgcttcaag gcctcgcgcg
    26941 cgttcggcaa tgccgtcaag gagatcctcg gcggcgatcc cgacatcgtg ttcgagcacg
    27001 tcggccaggc cacgttcccg acctcggtcc tcgtggtcaa gccgttcggc aaggtcgtga
    27061 tctgtgccgg caccaccggc tacaacctcg acttcgacgt ccgctacctc tggatgaggc
    27121 agaaggagat cctcggctcg cacttcgcca acgcgtggga gtgcacgaag gccaacgagc
    27181 tgatcgcgtc gggcgagatc cgcccggtgc tgtggcgcac gatgggcttc gacggcgtgg
    27241 ccgaggcgca ccagatcatg aaggagaaca agcacctcgg gaagatcgcg atcctcgtgg
    27301 gcgccgagga gaccggcctg ggcaagacgg ccgagggccc gggggcgatc tacgcggagg
    27361 tcggcgcctg atgatgcagg cggacgagaa catcgccgtc gtctactgga gcatcaaccc
    27421 gttccgcggc gacaagttcg aggacgcctg gcgcccggcc gcgcgggcgg cgctcgacta
    27481 cggcgcgacc tcgtggatgc tcttccgctc gcaggacgac ccgctgagct tcatccagct
    27541 ggcgtcgttc ccgaccaaga aggacttcga ccgctactgg ttctccgagg agatcgccga
    27601 ggcgcgtgcc gccgcgagcg ggctctacca ggtgccggtg atcccgaagt ggctgcgcgt
    27661 ggttgactcg ggcgagctcg cccgagtcga gagctagtcc ccgagcatct cgtcgacgta
    27721 gggaccgagg atcgcgacga tcgcgtccgc ggtcgcgcgc ggcgacgcgg tgggcagcgc
    27781 cgcgtggctg atcgcgaggc gcaccagcga ctcgaccatc gtgcgcgtct cgcgcagcgg
    27841 cagggcgggc caggtcgcga cgatcacgtc ggccagccgc gccgtcacgt cggcgatcag
    27901 cggcccgccc cgggtggtca cgagcggtag cagctcgtcg ccctcgtccg acgacgagat
    27961 cgcgcgcacc agcgggttct cggcagccgc ggcgaggaaa agctccgtcg cgctcgcgag
    28021 cgcggcccgc gggtcgtggg cgtcggcgcg gatcgcctcc tcggtggcgg ccatgaaccg
    28081 ctccgcctcc cgcatcacgt aggcctgcgc cagctcgcgc cggccgccga agacgttgta
    28141 gagcgtctgg cggctgacgc cgccgcgctc ggcgacctcc cccatcgtca cgtcggacca
    28201 cggccggccg gcgagcagct ccccgaccgc gtccagaacc gtgtcgcgca gcaggccgcg
    28261 cgtggcctcg gccagcgtgg tggtcatgag ctaggcgccg aagcgcgcca ggtagcgcgc
    28321 gcgcttgcgc gggtggatgt tggcccgggt cacgtcgccc ccgtagtggg cgaccacctt
    28381 cgggtccatc acccgccgcc agagcggcgt gaggtaggcc agcaggatca tcgtcgcgta
    28441 gccggagggg agctccggca gcccgtcgta gtggcgcagc gcctggtagc ggcgcatcgg
    28501 gttcgcgtgg tggtcgctgt ggcgctggag gtggtagagg aagacgttcg acgcgacgtt
    28561 gtcgctgttc cagctgtgct ccggccgcgt gcgctcgtag cggccgtcgt cgcgcttctc
    28621 gcgcagcagc ccgtagtgct cgaggtagtt gacgacctcg agcagcgaga acccgaccac
    28681 cgcctggatg gcgagccacg gcaggatctc gaccccgaac acggcgacca gcgcgccgaa
    28741 caggacgacc gtcatcgccc aggcgttgag cacgtcgttg cggggcgaga tgaaccgctc
    28801 gcccgcgtcc gcgaggcgct cgcgctcgag gtgcagcgcg gagcgcaggc tgcccatgac
    28861 cgtgcgcggc aggaacgccc agaagctctc gccgagcttg gagctggccg ggtcctcggg
    28921 cgtcgagacc cgcacgtggt ggccgcggtt gtgctcgacg aagaagtgcc cgtagcccgt
    28981 ctgcgccagc gcgaccttcg acagccagcg ctcgtgcgac ttgcgcttgt ggccgagctc
    29041 gtgggcggtg ttgatcgcga tcccggacac catcgcgatg gtcatcgcca ggccgaggct
    29101 ctcgagcagg gagaggtcgc cgctcgtcca cagccagcac gcgagcagca gcgagccgta
    29161 ctggagcggg atgaacacgt aggtgcacca gcggtagtag ggatcccgct cgagccactt
    29221 gatggcgctg tcgggcgggt tggacgagtc cttgccgatc gcgtggtcga ggaacgggaa
    29281 gagcccgaac acgagcgcgg ggccgaacca ccacataacc cccagcccgg tgagctcggc
    29341 gaggccgaac gcgaggaacg gcgcgagcgg cacgatcagc ccgagcagcc acaggtagcg
    29401 cttcgggtcg gtccaggtgg cggtgagcgg ctggtttaca gaagctgtcg tcatgtcgcg
    29461 agagtttaca cgaatacgag ttttgtcaac cacgcggcgc cgggtggcgc cgggcggggg
    29521 cagcgggagt agcctgcggc cggtgaggag ggggatcgcg agagcggcgc tggcggccgc
    29581 cgcggcgtcg ctgatcggac tcgctgccgc catgccggcg agcgccgacc tgaacacgct
    29641 caaggcgagc tgccagcagc gtgacgccgc cgacggcgac accgcgaacg gcatcaccct
    29701 cccgttcacg ttctgcgacg acggcgtgcc cgacgtgggc gggcgcacgc cgaacatcgg
    29761 tgcggtcaag gcggtcgcgg tgccgcagcg ctacgacggc ttcgcggggc tgccgccgaa
    29821 ggtcgccccc gacccgaacg cgggcgccga cctcgacggc aacatcgcgc tcgacatcga
    29881 cgtctcgctg ccggaccccg cgcgccatcc gcccccgccc ggcggatacc cgctcgtcgc
    29941 gctcatgcac ggctgctgcg cgggcaacaa gacgagctgg gaggcccccg ccgtggacgc
    30001 gccgcgcgag cagtggcact acaacaacgc ctggttcgcc tcgcgcgggt tcgtggtcct
    30061 caactacacg gcgcgcgggt tcgtggacca gaacgaccat ggctcgacgg gagagaccca
    30121 gctcgactcg cgcctctacg agatcaacga cttccagcac ctcgccggcc agctcgccga
    30181 cgacccgtac ttccacgtcg accccgaccg cgtggtcgcc accggcggct catacggcgg
    30241 ggggtggacc tggatggcga tcaccgaccc gacctggcgc agcccggccg gcagggacat
    30301 gcgcctggtg gccgcggcgc cgaagtacgg ctggaccgac ctcgcctact cgctggtgcc
    30361 aaacgggacc cacttcgagg gagtgctccc caccaccgac cccgaacgcg ccgcggagcc
    30421 gttcgggacc ccgaaggcga gcatcgtcgc ggcgctctac accagcggcc ggaccggcat
    30481 cccgcccggg accggccccc actccacgtt cgcgccgaac atcgaccagg cgctcgcgtg
    30541 cctcgaggcc gatgagccgc tcgcgagcac ccccacgtgc ggcgcggcgc tggacgtcac
    30601 gctgccgggc ttccgcaggg accgctcggc ctactaccag agccggttct tcgagcggat
    30661 cgcctcggag ccgcagtcgc gcgtgccgat cttcagcgcc ggcaccttca ccgacaacct
    30721 gttcacggcg atcgagcacc ggcgcatggt ggacaggatc aagtcgctcg tccccgagta
    30781 ccccgtgcag gagttctacg gcgactacaa ccacttcgta cagaacaagc cgaaggagtg
    30841 gggcgacgtc tgcggcggcg accggcacgt ctgccgcttc gcggactacc cgagcggcga
    30901 cctgaacgcc gagcccgcgg gccggctgcg caccggcgcc accacgcgcc tgaacgcgtt
    30961 tctcgaccac tacgtgaagc cgcagggcaa cccgagccag ccacggccgg ccttcgacgt
    31021 gaccggctcg ctgctgatct gcccgcagaa cgccagcgcc gagttcccgg ccgacgagcc
    31081 cggcgagcgc ttcaccgcgc cgaccttcga cgagctcgcg ccgaacacgc tcacgatcgc
    31141 cgccgccggc gctcaggcga cctccaacgc ggccgccgac ccgcacgccg cgcaggcgga
    31201 gccggtgagc aacagtgtcg ccaacggggg gcgctgcccg gtcgcgacca cgcccgccgg
    31261 cgccggcacg gcggtctacg actccgatcc gctgccgcgc gactacacga tgctcggcct
    31321 ctcgcgcgtc cacgccacgc acaccggcag cggcgacgac atccagctca acgcgcgcat
    31381 gtacgacgtc ttccccgacg gcagcgcggt gatgatggac cgcggggcgc accgcgtgac
    31441 cgacgccaac ggcaccaccg tgctcccgat ccagggcaac ggctggcggt tcccggcggg
    31501 ccaccggatc cgcatcgagc tgacgcagaa cgacgcgccg tacatgcgcc agtcgaaccg
    31561 gccctccgcg ctgctgattt ccgggatgaa gctcgaggtg ccggtccgcg aggcgtcggc
    31621 cacgatgggc gcggaggccg ccgggagcga gccgcccggc gtcgacctca gctcgccgcg
    31681 gctggccagc gacacgagcc gcgacccgcg cttccggctc gggctgcgct cgaccggcgg
    31741 cgcgagcgac cactacgagc tcgaggtgcg caacctgcgc agcacgacct ggaagcgcct
    31801 cagctcgaac ctgcgcgtgc ccgtgttctc gttcgcgggc tacttcggct cggcgtacga
    31861 gttccgggcg cgtgccgtgg gccgcttcgg cgagcgcggc gcgtgggaca ccgcccggac
    31921 ggtcgtgccg ttcgacgact cgcggcggcg cagcaagccg agcttcgggc gcggctggtc
    31981 gcacgtgcgc aacgcgtccg cctggggcca gcggctcagc cgcgcgtcag ggaagggccg
    32041 cggcatgcgg ctcgcgttca agggagcggc gaaggtctac ctgatcgcgc gc
//
LOCUS       DASVPL010000188        32086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_217672, whole genome shotgun sequence.
ACCESSION   DASVPL010000188 DASVPL010000000
VERSION     DASVPL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 32086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32086
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217672"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2902
                     /locus_tag="VF517_09725"
     CDS             <1..2902
                     /locus_tag="VF517_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8053261.1"
                     /translation="TLEDLALVPSDRGSVPLGEGEVRVAVRAAGLNFRDVLIALGIYP
                     GDAPLGSEAAGVVLELGPGVEGFAPGDSVMGLIGDSFGTVAVTDHRTLVRIPRGWSFV
                     EAASVPAVFLTAYFALVDLAEVKRGDRILIHAAAGGVGMAAVQLAHHLGAEVYATASP
                     AKWDAVRELGIADDHIASSRDLGFRDRFLEATGGEGVDVVLDALAREFVDASLELLPR
                     GGRFVEMGKADVRDPDEVARDHPGVRYRAFDLIEAGPERLQRMLGELVEMFDQGVLRH
                     APIRTWDVRRGADAFRFLREARHIGKVVLTIPPAFDPDGTVLVTGGTSGLGALVARHL
                     AEHHHARRLLLVSRRGREADGVGTLVEELAALGCESTVAACDVADRDALAELIGSLEH
                     PLTAVVHAAGVLDDGVVAALTPEQLDRVMRPKVDAAMNLHELTRDGTLSDFVLFSSAA
                     AVMGSPGQGNYAAANSFLDALAQHRHGQGLAASSLAWGLWADAGGMSAGLDEQEVARL
                     ARMGVQPLASSEGLALFDTARQLGEPALVPIRLDTAALRAGAAAGMLPPLLRGIVSAP
                     ARRDQDGGASLARRLATADGNREQVILDVVRGHVAAVLGRGSSDAVEPDREFKELGLD
                     SLAAVELRNRLTQATGMRLPTTLVFDHPSAAAIAEYVHEHLGDQPSQRSAPVAQGDGD
                     GTLTALLRHALDSGALVDAVPLLTEASRFRPSFESASELDDDGGGYVVQMASGDEGPK
                     LVCVPSFVVGSGPHQFARFASAFDGERDVFACALPGFRGSEPVPGTWDAAIDVLAESI
                     RGAVGDDPFVLVGYSTGGVLSHSLARRFEREGPAPAGLVLIDTPTPEADDDVNNHLFS
                     SVVSVILERAHEAIAVDDANWLAMGTYIRLQHDWEPDPIDTPSLLIRAGVPLGVADAD
                     QPSWPAWEVSDDVTEIQGDHFALIETAVAATAEATGGWLGALAGRTSGVGAG"
     gene            2961..3470
                     /locus_tag="VF517_09730"
     CDS             2961..3470
                     /locus_tag="VF517_09730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8053262.1"
                     /translation="MPERVKGETRTRTVSWEDPAPSVPRLIEMTGLEAMQAVVSGDLP
                     SSPFVEVIGIRAIEAEEGRVTMAVRTEDYHANPAGGVHGGLAAALLDSAMWSAVLTTM
                     PQGTFCTTLQMNVNYVRPIPMDDEEVRAEAHVVHGGRRTATAEGSIHDSNGKLCAHGT
                     ATCVVVGGS"
     gene            complement(3475..4929)
                     /locus_tag="VF517_09735"
     CDS             complement(3475..4929)
                     /locus_tag="VF517_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8053263.1"
                     /translation="MLNAEKADGSTVSDGNGSCPHMAAPTPRPTQDTVAEWVKASNAE
                     SGLPPGPSMSSVMQTLGIWSRPTAFFERCRARYGTPFTLHIRVPPTPFVMLASPDEIK
                     QMFMAPADVLWAGEGSSSIEKYTGQTGLACLDEDRHLARRRLVNRSMQGETVQRASAD
                     IAAIAERDVASWPRGEVIELYPWVHRLTLKVVFRIVFGPEPDRRLDELLDVVMSMMDI
                     NDKPTSVVPVHTLSPRAARILTAFRPSGIRRFQELSARADKLIYEVLEDRRRDGVEGE
                     DMVGLMLAARDEDGSPLDLVEIRDEVMTNFVAGTTTTAASISWAVEHLTREPEIRQRL
                     VDEIKAGESDAYLTATVHELLRRRPPLPTSIPRLVVKPIEIGGREYKPGVHLWGSPYL
                     LHHDESVYPDPYSFRPERFLDNAPGTYTWIPFGGGRRRCLGRAIADLEIKCVLREILG
                     NYELSRDTPQPERVRAKLVTAVPSRNARVALSPA"
     gene            complement(4982..5593)
                     /locus_tag="VF517_09740"
     CDS             complement(4982..5593)
                     /locus_tag="VF517_09740"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX8053264.1"
                     /translation="MTPEMRLPNSAHTSRPWRIHELTRDFRLEDVWALPTPGGPDDFP
                     RLVERMAAGDPEKDSSGIGRALWSIRWKVGELLGWDDEDAGARSGRPTLRDRLPADLR
                     EGPAGPEFETLPFTSLYQLDDEFAAEIVNKTVHGVMHLSWVPDESGGYRGEMAVYVKP
                     NGAFGNAYMAAIKPFRHLLVYPPMMKKLGRDWRRSGASTPTPA"
     gene            complement(5590..6222)
                     /locus_tag="VF517_09745"
     CDS             complement(5590..6222)
                     /locus_tag="VF517_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053265.1"
                     /translation="MSRTSVGELSASMAVLGLVIEEPEDASCVAQRLSERFRPARFAP
                     STAHMTLPRLEAQGLVRRVTAPEDARDSAQGRYEATEEGIDRFREWLRTSPDGLPALR
                     EALHARIDLAQPEDLERVIEITDRERLACERECAAARGRLAEARQVARFKGTNVDWSE
                     QVREVVMVDEAMIWAYRAARLERLHARLVDLRGQHGEEYEHRGEAAKLAS"
     gene            6490..8013
                     /locus_tag="VF517_09750"
     CDS             6490..8013
                     /locus_tag="VF517_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053266.1"
                     /translation="MTLPSILVASFAVSVDTTIVNVSLPTLVRELDASTSELQWIVGA
                     YSLVFAALLLAAGSLSDRVGRKGVLLAGLAVFGLASAAGGLTDSPEQLIVARGAMGLG
                     AALIFPATLSLISNIFPERDERARAIGLWGATTGAAVALGPIAGGWLLETFDWSAIFF
                     AMAPVAALAALLIGRTVPTSRDPDAPRADRPGLVLSASAIALLIYTIIEAPDYGWGAG
                     RTVAGFAVAAVLLVLFVLWQRRASAPLVDVSLFRNLRFTAASASVTVVFFSLSGFLFL
                     ITQYFQFFKGYGPLSTGVRMLPVALSLAPMSVIGTKMAVRYGTKVVVAIGLVCLAVFF
                     VWVSTDSETTGYFVIASQMVLSGIGMGLSSAPATEAIMGVVPKAKAGVGSAVNDATRI
                     LGATLGVAVIGSVYASVYSSRLVDALPAGVPGPQADAAEDSVGAALALAGRAGPALGD
                     RIHEAAASAFFDGMSSGLLVAAGVAAVGAVVAASLLPAQPVVSHEDVPVAPPVPAVE"
     gene            complement(8017..8955)
                     /locus_tag="VF517_09755"
     CDS             complement(8017..8955)
                     /locus_tag="VF517_09755"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX8053267.1"
                     /translation="MAPELDPDAQMILEVVIASGQGSLHSLPVEEARERVRAALVTKG
                     APFPLHSVEDVSVPAPTGALPLRLYRPREGRLPVALFLHGGGWTVNDVDTHDDLCRRL
                     AKRSGWMLASLDYRRAPEHKHPAQLEDAHVAYRWLLDNAESIGADAAPPALFGESSGG
                     ATAASLTLLLRDQGEPMPPYQVLAYPVADDFDRWPSYRERGTGYILDRALMEWYFGNF
                     RPPGPAADHQYLVPLATEDLSGLPPAFVLTAEFDPLRDEGVAYAKRLAEAGVEVEHVH
                     VDDQMHGFLLLGRVVPRASALVDRTADALAAHARRG"
     gene            8982..9836
                     /locus_tag="VF517_09760"
     CDS             8982..9836
                     /locus_tag="VF517_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8053268.1"
                     /translation="MPDIEQAALVAISDLHVEATDNRSFVEGLRPRHDDDWLIACGDV
                     GEIMSDVEWALATLARNFEKVIWTPGNHELWTSPADEVQLRGDARYRHLVSYCQGLGI
                     ATPEDPFPVWSGAGGPVVVAPIFTLYDYSFGVNVAPTKEEALARAHDAGVVCSDELVL
                     HPDPYESREQWCRARVEYTEGRLTAMAAEGLPSVLAGHFPLLAELTSPLMHPEFAQWC
                     GTLATADWHTRFRAEAVVYGHLHIPRTTTHDGVRFEEVTLGYPLQRRRWVNRRPVPRP
                     IVPFGGGE"
     gene            9833..10480
                     /locus_tag="VF517_09765"
     CDS             9833..10480
                     /locus_tag="VF517_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX8053269.1"
                     /translation="MIERLVPESVAVVSTRGDLPDVELFAEEERSVGRAVEKRRREFT
                     TARGCARMALERLGVAPVAIPNGERGEPVWPGGIVGSITHCTGYRASAVARVEDVLSV
                     GIDAEVHERLPEGVLEQIAFGTELATVAGDGGGVCLDRLMFSAKEAVYKAWFPLARRW
                     LGFEDVEMTIDVPVSSFRARLLVPGPVVGGVRLTEFRGRWAVEDGIVAAAVVVLP"
     gene            10548..11165
                     /locus_tag="VF517_09770"
     CDS             10548..11165
                     /locus_tag="VF517_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053270.1"
                     /translation="MAPHEVARSSLPASGDLGADLIPFGDALSDRPRSSCPDADAAVL
                     VVEVAHADSLTAGRSALELGALLAKFFRTVAEVTEAHGGYAERDDLCAVRCVFGDPAS
                     STCAADAALAAARELRDRLVGELPELAFTIGISVGAPGAGWIRARHRFEPLVICAPAS
                     EARRLCGQARRGGPPVLASERALARASATEACRWSSAAPAAHSIR"
     gene            11218..13200
                     /locus_tag="VF517_09775"
     CDS             11218..13200
                     /locus_tag="VF517_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8053271.1"
                     /translation="MRGLTRTCVVWGALALLLAMPSLARAGDLSPSSFDPGPVEYPNV
                     VKESDVPIVMRDGVRLYADVYRPADADGNAAPGRFPVVLNQILFNKGTTRLDGTPAGQ
                     IEEGASGYSPIFTKHGYVQVIVDARGTGSSQGEFSMFGPDEQRDGVEIARWLVRQPFS
                     DGRFVAYGASCMAIYGTFLAAQHPPGLKAMFPIVPMDDIYRDVLWHGGGLDVPFLAIW
                     HGMITSTKTRPPLYSASDPEAAIEVLRSRVSPSIVPTEILTEPYDNDYYRTRSPGRVV
                     SQVNVPTFVVGGWYDLMQRGTPRIYNQLQLPPGQKQLMMGPWYHLTGGQGLGQPGAPP
                     PIDVLALAWFDRWVKGERNGIENYGPVTVNQLGANRWETYRQYPRRDVQYKRFYLSGE
                     KSGTAQSLNDGSLSTDAPRSAGSDDMPGNSVNGLCTRSTVQWSVSFVPPGQPCETDNR
                     SQEATSLTYTTPPLADTMHLSGPLSLTLNGSTTARDTTWVATVTDVGPDGSSNQITSG
                     WLIQSRRALDAARTTFAPNGDPIVPYHPFTRESVLPVEPGVRDSMAVEIFNTDAVLEP
                     GHRLRVTLSSGDVPHILAPPDTVLAGVGGMSTVHRGGDAQSFLTAGLAPLGPEPAPKP
                     TKLQRCRSKAKHAHKSGKRRRAALARCSARYGNRAT"
     gene            13217..14188
                     /locus_tag="VF517_09780"
     CDS             13217..14188
                     /locus_tag="VF517_09780"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX8053272.1"
                     /translation="MSVSAETIAQGPPRSAARRFASAASLDLPMIAMLAAFVAEPSTR
                     DFALVNLLVQGVLFLFGACLPAHKTRLMAFVDGIWPWGLVALGVQALLFGDTGSPVLL
                     VVAAIYLAMGLRGGLWAMVVAFTTRRREDLPRYRYRRLLWKREGYRSKTVPMQHEIVQ
                     QAFCNVSFLAAPALLAVGDRHADIGPLVVAGAVLWAVSWALESLADVQKSRFAKRSAA
                     EGTAGTCEDGLWRYSRHPNYFFQWLQWHGLILVALPSLIAVADDFALLPWVGAAAALA
                     AISAGMYYVVVHYTGAIPAEYFSAQHRPGYRDYQRRVNRFVPGPTRG"
     gene            complement(14185..15165)
                     /locus_tag="VF517_09785"
     CDS             complement(14185..15165)
                     /locus_tag="VF517_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053273.1"
                     /translation="MDSRSATPEDLPPLTLSLLRELDLDASPTPGRAAHISAASGVAR
                     RGDYVYVIGDDELGLGVFRMSKEAPGELRTVLRGELSADHDERKREKPDLEALTVLPP
                     FGEHPYGALLGLGSGTKRQRERGFVWGLAADGSLSGEPVELDLAPLYARLRESAPELN
                     IEGAAALGDRLALFHRGNTEQGLNIVAEVPLGEFVEGVRAGGPVDCGDVRTRAYELGD
                     LDGVPLTFSDATPIADDLVVFTASAEPGSDPHGADGEIRGSVVGTIDGDGHVTRLRTI
                     DRRWKVEGVYASVDARVIDFLFVCDQDDPAAASPLLSAAMPIEPRFEGLG"
     gene            15219..16640
                     /locus_tag="VF517_09790"
     CDS             15219..16640
                     /locus_tag="VF517_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8053274.1"
                     /translation="MSTAVEPRTTGPHDGLVGEAPPSPAGPLENGDGAGLGSPPTIAL
                     PRLVQALRFNQRQIQFVFGARRRHGEVFRMTGLLPNGPVITDHPDHVRSLFTAKPEQA
                     PSLTGESSLRPIVGSNSVLTALGERHMRQRKLLLPSFHGEAIERYVQMISDATEREMA
                     DWPVDRPFALVPRMQAITLDVIMGGIFGIEGRPESGTPEHGLRATTRTLVAGSTNPVA
                     QLGELINTGRDEAVGPLRWALAVLDRQVYAVIAARRRSADLEERGDILSLLLQARTED
                     GEALTDEELRNELLVLVLAGHETTANQLAWTWERLVRTPAAYDRLREAVRSGVDAEEH
                     VEAAIVEGMRVRPVVPMIGRRVAVPWRFGPYAVAPGTPVGMSILLLHHRDDLYPDPFA
                     YRPERWLGRKPGTYEWIPFGGGIRRCLGAALAMAEQRVVLEAMVRTLDLEAADPEPER
                     AAHRNVTMIPARGARVVVRARRG"
     gene            16728..16925
                     /locus_tag="VF517_09795"
     CDS             16728..16925
                     /locus_tag="VF517_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053275.1"
                     /translation="MNQDKPADGRKAWQAPEIVEVGGVETVTTYDDGNLRDNQGDATP
                     KYKVFTRTKTESPESELPHGD"
     gene            16968..17651
                     /locus_tag="VF517_09800"
     CDS             16968..17651
                     /locus_tag="VF517_09800"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX8053276.1"
                     /translation="MRYARVDGLGVFLDLRTSTYKVLDEVATSMLDALTGAADPEAEL
                     AGWCREYEIDPETARAELARFADTCVSDGLLGARGRDDGEPAPPSAEPLASAPRGPRV
                     GRAVHALAATWRSLRADGFGPTYERYARIPVAPAASAPGACVRAFVRAENVWPSKRAP
                     DDCLLRSLALFRYLRGEGVAAEHVIGVWRFPFLAHAWVECGGAPMLDERVSGFTPLAR
                     IAAADAAAR"
     gene            17648..19399
                     /locus_tag="VF517_09805"
     CDS             17648..19399
                     /locus_tag="VF517_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8053277.1"
                     /translation="MTSAHFSGALSAPNRLAALDGEAIGGPPSAGCRCLFRGWVADAP
                     ALRRRLGLADEGGAEPGGAERLLAAAFREWGDRLQARVDGQFAAVVLDAAAGTALLTH
                     DALGIAPLYWRERSGRLEFSTRLRDLVDARAAADLDDEYLADFLAFGVPSSRRTPYRA
                     IRRLMPGTSLRWSGGRVTELRTWSASDVEPVRFRDNREYEERFEELVGSAVGGALDTG
                     RPTWIALSGGTDSVTVAGAAARHGGGDLRAYSVVAPRWQESDESRWIRKTVDALGLPW
                     QPIDAEDVLPFARMPTEFCGEPNQSIVNLGLIDTVQDVLGASGSRVLLTGKGGDGFAG
                     ASSGDVPAHLADALFEARPLTALRETLAWKAAADDVRSLSFWLRRGLIAPSLRHLGGR
                     RARVHTQRLTAPWLRPDYERTLRVRARAQRSPSPRCRTPGRQMLLDELWLCSAQMEPA
                     ATIGHEVRHPLFHRPLFEFQWAIPWQQKLLPRCDRYLQRRALRGVVPDSVRRRVSSAT
                     GTRALVEGLRRSREWQDYLCERPRIAEHGIADAERWRTAIKQASVGQTHGDHLLVAGI
                     AVEVWLKQLDEHGGGAG"
     gene            19396..20619
                     /locus_tag="VF517_09810"
     CDS             19396..20619
                     /locus_tag="VF517_09810"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX8053278.1"
                     /translation="MREGLTSGARVRWEDGELELRPRERFLPLLRAGVEAAPDRPEPR
                     RRLARLLLQTEQLEELEELATPALDDPGADPEVLHCLGLGALATERPELALRALRRAV
                     DLGFTHSLGALANVLRDLGRDDEALTAALQALELDPSNHSASGVVARILLERGERERL
                     WSLCQEIRRGGGWGAYLPSAMAVCARTGEQHREVERLVGREAWFLRRDLGLAPEFSRR
                     LAEELLDLMGTGDLPRRKASTGTGRRIDQLQVVARTNPVVAELFAALRAAIEEYLAER
                     EDALADELLGQQLGGRLPDELSLGAWALAMRGDGHEEWHMHPSGWLSGVYYVEVPDMT
                     ASAAEHPGAIDFGPFPFTGVEEVPSWPPWRIAPRTGEMLLFPSSYAHRTWPTGVDEDR
                     IVVAFDVLAAHPAEA"
     gene            20619..20912
                     /locus_tag="VF517_09815"
     CDS             20619..20912
                     /locus_tag="VF517_09815"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX8053279.1"
                     /translation="MASAELDPAAPIARSPEAVFAPGPPENFIMHLTSGRCVSVDDAG
                     VRIWELLESPRSLSELVDRLAQEFSGDRDAIAADVRAFVLALRDCELALPSPQ"
     gene            20909..21385
                     /gene="greA"
                     /locus_tag="VF517_09820"
     CDS             20909..21385
                     /gene="greA"
                     /locus_tag="VF517_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX8053280.1"
                     /translation="MTGPGSEAITAEGLRALEAELHELETAGRLAMSERIKAARELGD
                     LKENAEYHIAKDDQAHLETKIKRLRQRLLAAKVVEADADDATFAFGRTAKVLDEATGT
                     VHEWTIVGPTEADIKAGKLSSESPVAQALLGRAPGDTVGVETPRGTRSYVIKKLVG"
     gene            complement(21392..21844)
                     /locus_tag="VF517_09825"
     CDS             complement(21392..21844)
                     /locus_tag="VF517_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8053281.1"
                     /translation="MRDQTRARRRGLVYDALRVVEIDPARPLALDDVAREIAASRRSL
                     QRAFEDEGITFRQAVAVARIRRARALLAEGSLPVYVVAERVGYMSKAEFTKAFKRHVG
                     VRPSEYKRTMREERMQHADLRAPIAPVRRGFATPARAPAAAVAQHASV"
     gene            complement(21909..24020)
                     /locus_tag="VF517_09830"
     CDS             complement(21909..24020)
                     /locus_tag="VF517_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8053282.1"
                     /translation="MRGNRMLMLAAVALTALAATASPALAAEPTKKALTFDVVVGPQD
                     DTECTVTANLFTPAGASKDDPVAAVMATNGFGGGKSDYDALGLAYAKRGYVFLAYSGL
                     GFGNSGCKVTLDDPDWDGKAGSQLVSFLAGTKAANDGTKIDFVRADAPGDPRAGMIGG
                     SYGGQIQFAIAGIDKRLDTIVPSITWNDLSYSLVPNNTDFERGVTYRTPGVTKSDWPA
                     LFTALGLSVGFQQAVQNQDPSHLGACPNFTDQVCTSLVTSASTGYADEATLALLRHAS
                     VATYMSKIKIPTFLSQGQSDTLFDIQEAVATYQALRAQGTPVKMLWRSSGHSGGNIGG
                     ESNSTNLESAYESRMALEWFDYYLRGIGDPPTLDFSFLRDWVKYEGDAAPAVGNTRGY
                     PASRDETVFLSGTDQLVPDRAGVKPGSASFAAAPGATGTGGAAIAQPPAGNAAGTTAS
                     YTTAPLTEDVDVVGVPRLTVKLDAPTFAESQAQGPASKLVLFVKLLDVDASGAEVLPR
                     NQLSAVRVADVTKPVEIELPGIAHRFAKGHSMRVVIATSNSTNHGNNLAGPVTVVADP
                     AAPSTLTIPRLGAQAGPVGSAPSGTTPFAPPAGAPAAQPAGSGARARALTAASFSTNK
                     RCVKRRAVAFRIVRHAGSNRAASAVVTVNGKTVRKLRGAALPAKVRLERMPKRGAYRV
                     QVTVKTVGGKTLRSARTFRAC"
     gene            24118..24489
                     /locus_tag="VF517_09835"
     CDS             24118..24489
                     /locus_tag="VF517_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8053283.1"
                     /translation="MLRRADEVRMERKPGRAKGKGEFECRYLVEEGENENGIQFVHDD
                     TLDPGASFGTHRHIDSEEIYLVLDGTGQAWVDDDRWDVGPGDVVTLHKGQSHGIEANA
                     DGKLRFLAIGIGGPKRKYEVP"
     gene            24510..25682
                     /locus_tag="VF517_09840"
     CDS             24510..25682
                     /locus_tag="VF517_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053284.1"
                     /translation="MGRPAARSADAGAARHHFAVDVLRDAPGDPAAAVEALVEAVASR
                     LGPPGADALARVGLFLPAPIRFEDAEPIVARVLGEPCPPLELFVQPVVGGGAAALTWS
                     VTGDGVRVERAEPSAARVVAPEVELAVLGHAAGEPPAATAGDFAAGLRRFDTRLQGLG
                     LRFADAIKLWTVFPSTPELGTEQAFNEFNRGRVEVFDALDFHLSEGTGAPRYPASTGV
                     GALDATAPLSGVALAGGARGVQVENPAQVSAFRYPTERIDKPALFSRAIAVDLGTHVM
                     TYVAGTASTVGAKTAHVGDPAAQAEQTLNNIDTLITAENLARSGTPVTEGGLATLSHI
                     VVYAATDEAEAAAVEVCARMVPPEVPMLMVRSRLTRDELLFEADGVAFAPSAAPAD"
     gene            complement(25679..27409)
                     /locus_tag="VF517_09845"
     CDS             complement(25679..27409)
                     /locus_tag="VF517_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8053285.1"
                     /translation="MSTSSTNARPGGTETVARTGLLDWLEEPRAGHGLRFAEDGGGWA
                     MWEYPRLAALAGGTAEQIAELRHARTGVVSIVTRSGPEFIAAFVGALAAGNTPSPLAL
                     PPFVRDRSQYVEHAASILRAAEPVLVLVEAGFEELIEEAAELAGVPGRVARIEASEGP
                     APAPPGRDRAELALLQFTSGSSGRPRAVRVTWENLEANVAMIRRWLGMRPEDATATWL
                     PLYHDMGLIGCMVTPMVNQSDVWVMRPDQFIREPVRWLECYGVHGAQLGVAPNFGFGY
                     VNKRLSDEQLEPMDFSQWRVAIVGAERLDADVLGRFARRLEPRGFRASTFLPAYGLAE
                     ATLAVTGVPLEDVPRAVRPDWDDARFGEPLPVLASAMLTDAERIGSGEGWLVGCGPPH
                     PEVGVAIVDEDGRDLPEGALGEIAVTGPTVADDYLGEPGASTRFENGRLLSGDAGFVY
                     EGELYVMGRLGDSVKVRGRTVFVEDVEARIAALGVPKGKCVVLAGTHAGANLIAALAE
                     APAGPWVEKAAAMLEREGDASSRVLVLSGDPGSIERTSSGKPRRRVMWQRIVRGELPG
                     EVVHEGSGTG"
     gene            27450..29021
                     /locus_tag="VF517_09850"
     CDS             27450..29021
                     /locus_tag="VF517_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8053286.1"
                     /translation="MAGVHRGATYADLILAARRRYPDEPVDRTGQLVEAVIGAGVERG
                     EGVALLATGNPHASAATAAALAAGARVSALHPLASADDQAFVLDDAEISTLVYDPVHL
                     GDRAADLASRVPRVERLLALGPSADAPDLIELADRLPACRPRSLAEEGDLAFVHYTGG
                     TTGRPKGVAVSHAAMVHGALMVLAEWDWPAEVRFLACTQMAQVLLVPVRLLGGSVHYS
                     MFDPELVLRTIERERITCMYLVPSMIYALLDHPGIGDADLSSVESIFYGASTIAPARL
                     EEALETFGPVMCQIYASTEATHTVTVLPKRDHDPAVPGRLESCGRPAAALDVEVHDED
                     GRALAPGEVGEVAVRGRSLMEGYWRRPELTAAALRGGWYRTGDVAFQDERGFVTIIDR
                     RGDAIIRNGFNVYPREVESVLASHPAVLHAAVFGVPDGTAGEEVMAAVVPRAGAHVSV
                     DDLARLVHERKGVRYVPGAIEVVGAIAATPSGKPDRRRLRELAAAAKPERAAAAEPEQ
                     AEPERAAAAARPEPA"
     gene            complement(29018..30799)
                     /locus_tag="VF517_09855"
     CDS             complement(29018..30799)
                     /locus_tag="VF517_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX8053287.1"
                     /translation="MSAEPAVLQHLFEGAVERHAGRPAVGLGDDVWTYEELDRRASRV
                     AGWLAEHGVGSGDLVALLVERSSDYAAAMWGVLKAGATCLPLDPTDPWSRVAATLRLA
                     GATVAVARAEQVPGAAGELGMRVLDLRWDSPELTRQPDRVDPAPGEPGDLAYVFYTSG
                     STGEPKGVMLSHATCSAFIPWVAESYDFTPEDRHFLKTTVSFVSVVRQLVWPNVTGGY
                     AAILPAGRERDLRAVAEIVRDERVSILGFIPSSFAHFLAQADPRDMGAVRHVLCGGEP
                     LTKSLQLTAFDRLPAAEIHNLYALSEAPLVMHWRCTREDERPVAPMGDPIPGVEIAFA
                     DPDTGAPANGDGSAELYVGGVAPASGYLNAPELTAERFGAQPGGGGEGWLRTRDLVRR
                     AGDALEYAGRADSLVKVRGFRIEPGEVEAALNAFPGIAQCLVTMWEPDEGDRRLVAYL
                     VCDGDRVPETATLRDFLRERVPAYMVPSLFIGVGAFPIGARGKVDRAALPDPATATSS
                     GERVFVAPRDELEQEVADLWREVLKVPSVGAFDEFVDIGGESLLALQILEELERRAGV
                     EIPLEEFAEASTVAELAGVVRAARGPA"
     gene            complement(30796..31815)
                     /locus_tag="VF517_09860"
     CDS             complement(30796..31815)
                     /locus_tag="VF517_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX8053288.1"
                     /translation="MAVAGDTRRPAGDWLRSLRELVPIPEPYRDESMDRAAALEVLRC
                     PEEVLDRVVAEGFPSSGEGEAQRFDRRDVYNLALHSGSGRSLPELGVAFLGRLGREGE
                     QAWSEPRTWRIGAQIRCPRREGCGADPAWAFGRPVPEELGGELAESAPPGGSDPDADV
                     YEWSGEPGSASLSATIATRGSVERLVSDEVRDAFMAVVEDYRFQVLPPALRTDLDSVE
                     RIRAADCIAATGVLVRDCRAAGLEAEPQSGFLLGLFGFGGHRWVRVRDSDGRWKSLDP
                     TLAMIAALGGHDSGDFLEYCRGSILNRVVPCNTTPDGEVAGHSCDGESRLPDLAIRAR
                     RHDTD"
     gene            complement(31815..>32086)
                     /locus_tag="VF517_09865"
     CDS             complement(31815..>32086)
                     /locus_tag="VF517_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX8053289.1"
                     /translation="LLRQVQGGALALLAEAALDGAVASMLPASGERATVELRVDFLRK
                     APAGEGPLQADATLAQRGRNFTFGSCTVSSADRGRVAAATSVHRH"
BASE COUNT         4160 a        11705 c        11875 g         4346 t
ORIGIN      
        1 cacgctcgag gacctcgcgc tcgtgccctc cgaccgcggc agcgtgccgc tcggggaggg
       61 agaggtgcgc gtcgcggtac gcgcggccgg cctcaacttc cgcgacgtgc tgatcgcgct
      121 cgggatctac ccgggcgacg cgccgctcgg cagcgaggcg gccggagtgg tcctcgagct
      181 gggccccggc gtcgaggggt tcgcaccggg cgacagcgtg atgggcctga tcggcgattc
      241 gttcggcacc gtcgccgtga ccgaccaccg cacgctcgtc cgcatcccgc gcggctggtc
      301 gttcgtcgag gccgcgtcgg tgccggccgt gttcctcacg gcctatttcg cgctcgtgga
      361 cctcgccgag gtcaagcgtg gcgacaggat cctgattcac gcggcggccg gcggcgtggg
      421 gatggccgcc gtccagctcg cccaccacct cggcgccgag gtgtacgcga cggcgagccc
      481 cgccaagtgg gacgccgtgc gcgagctggg gatcgccgac gaccacatcg cctcctcccg
      541 cgacctcggc ttccgcgacc gcttcctgga ggcgaccggc ggcgagggag tcgacgtggt
      601 gctcgacgcg ctcgcgcgcg agttcgtgga cgcgtcgctc gaactgctgc cgcgcggcgg
      661 gcgcttcgtg gagatgggca aggccgacgt acgcgacccg gacgaggtcg cccgcgacca
      721 ccctggcgtg cgctaccggg cgttcgacct gatcgaggcc gggccggagc ggctccagcg
      781 gatgctcggc gagctcgtcg agatgttcga ccagggcgtg ctccgccacg cgccgatccg
      841 gacctgggac gtgcgccgcg gcgccgacgc gttccgcttc ctgcgcgagg ctcgccacat
      901 cggcaaggtc gtgctcacga tcccgcccgc cttcgacccc gacgggaccg tgctcgtcac
      961 gggcggcacc agcggtctcg gcgccctcgt cgcccgccac ctcgccgagc accatcacgc
     1021 gcgccggctg ctgctcgtca gccgccgcgg ccgcgaggcc gacggggtcg gcacgctcgt
     1081 cgaggagctc gcagcgctcg gctgcgagag caccgtcgcc gcctgcgacg tggccgaccg
     1141 cgacgccctc gccgagctga tcggctcgct ggagcacccg ctcacggcgg tcgtgcacgc
     1201 ggccggcgtg ctcgacgacg gcgtggtggc ggcgctcacg cccgagcagc tcgaccgggt
     1261 gatgcggccg aaggtcgacg ccgccatgaa cctccacgag ctgacccgcg acggcacgct
     1321 gtcggacttc gtgctgttct cgtcggcggc cgcggtcatg ggcagcccgg gccagggcaa
     1381 ctacgcggcg gccaactcgt tcctcgacgc actcgcccag caccgccacg gccagggcct
     1441 ggccgcgagc tcgctcgcgt ggggcctctg ggccgacgcc ggcgggatgt ccgccggcct
     1501 cgacgagcag gaggtggcgc ggctggcgcg gatgggcgtg cagccgctag cgagctccga
     1561 gggtctcgcg ctcttcgaca ccgcccgcca gctcggcgag ccggccctgg tgccgatccg
     1621 cctcgatacc gctgcgctgc gcgccggtgc cgccgcgggc atgctgccgc cgctgctgcg
     1681 cggcatcgtc agcgcgcccg cacgccgcga ccaggacggc ggcgcctcgc tcgcgcgcag
     1741 gctggcgacc gccgacggga atcgcgagca ggtcatcctc gacgtcgtgc gcggccacgt
     1801 ggccgccgtg ctcgggcgcg ggtcgagcga cgctgtcgag cccgaccgcg agttcaagga
     1861 gctgggcctc gactccctcg cggcggtcga gctgcgcaac cggctcacgc aggcgaccgg
     1921 gatgcggctg cccacgacgc tcgtgttcga ccacccgagc gccgccgcga tcgccgagta
     1981 cgtgcacgag catctcggcg atcagccctc ccagcggtcg gctccggtcg cgcagggcga
     2041 cggcgacggc accctcaccg cgctgctgcg ccacgcgctc gacagcggcg cactcgtgga
     2101 cgcggtgccg ctgctcaccg aggcgtcgcg gttccgcccc tccttcgagt cagcgagcga
     2161 gctggacgac gacggcggcg gctacgtcgt gcagatggca tcgggcgacg agggcccgaa
     2221 gctcgtctgc gtcccgtcgt tcgtggtcgg ctcgggcccg caccagttcg cgcggttcgc
     2281 gagcgccttc gacggcgagc gcgacgtgtt cgcctgcgcg ctgcccggat tccgcggcag
     2341 cgagcccgtg cccgggacgt gggacgcggc gatcgacgtc ctggccgagt cgatccgcgg
     2401 ggcggtcggc gacgacccgt tcgtcctcgt cgggtactcg accggcggag tgctgagcca
     2461 ctcgctggcc cggcggttcg agcgcgaggg gccggcgccc gccggcctgg tcctgatcga
     2521 caccccgacg cccgaggccg acgacgacgt caacaaccac ctcttctcgt ccgtggtgtc
     2581 ggtgatcctc gagcgcgccc acgaggcgat cgccgtggac gacgccaact ggctcgcgat
     2641 gggcacctac atccgccttc agcacgactg ggagccggac ccgatcgaca cgccgtcgct
     2701 gctcatccgc gccggtgtgc cgctgggcgt cgccgatgcc gatcagccga gctggccggc
     2761 gtgggaggtg agcgacgacg tgaccgagat ccagggcgac cacttcgcgc tgatcgagac
     2821 ggccgtcgcg gccacagccg aggcgaccgg cggctggctg ggggcgctgg ccgggcgcac
     2881 gagcggagtc ggcgcgggct gagaacatcc cagccgcgcc ggcgccacgg cgacgagtca
     2941 atcgacgaga ggagcgacac atgcctgaac gtgtgaaggg ggagacgcgc acccgcacgg
     3001 tgagctggga ggaccccgcc ccgtcggtcc cgcggctgat cgagatgacg gggctcgaag
     3061 cgatgcaggc ggtcgtgagc ggcgacctgc ccagctcgcc gttcgtcgag gtgatcggga
     3121 tccgcgccat cgaggccgag gagggcaggg tcaccatggc cgtgcggacc gaggactacc
     3181 acgccaaccc ggccggcggc gtccacggcg ggctcgcggc ggccctgctc gacagcgcga
     3241 tgtggtcggc ggtgctcacc accatgcccc agggaacctt ctgcacgacg ctccagatga
     3301 acgtcaacta cgtgcgcccg atcccgatgg acgacgagga ggtccgcgcc gaggcgcacg
     3361 tcgtgcacgg gggccgcagg accgcgaccg ccgaggggtc gatccacgac agcaacggca
     3421 agctgtgcgc gcacggcacg gccacctgcg tcgtggtcgg cgggagctag ccggctaggc
     3481 cggcgagagc gccacgcgcg cgttgcgcga gggcacggct gtcacgagct tcgcccgcac
     3541 ccgctcgggc tgcggggtat cgcggctcag ctcgtagttc ccgagtatct cgcgcaggac
     3601 gcacttgatc tcgaggtccg cgatggccct gccgaggcag cgcctgcggc cgcccccgaa
     3661 cgggatccac gtgtaggtgc cgggcgcgtt gtcgaggaag cgctcgggcc ggaacgagta
     3721 cgggtcgggg tagaccgact cgtcgtggtg gagcaggtac gggctccccc agaggtgcac
     3781 gccgggcttg tactccctgc cgccgatctc gatcggcttc accacgaggc gcgggatgct
     3841 ggtgggcagc ggcgggcgcc ggcgcaggag ctcgtggacc gtcgccgtga ggtaggcgtc
     3901 gctctcaccg gccttgatct cgtccaccag gcgctggcgg atctccggct cgcgcgtgag
     3961 gtgctcgacc gcccacgaga tcgaagcggc ggtcgtcgtg gtgccggcca cgaagttggt
     4021 catgacctcg tcgcggatct cgacgaggtc gagcggcgac ccgtcctcgt cgcgcgcggc
     4081 gagcatcagc ccgaccatgt cctcgccctc gacgccgtcg cggcggcgat cctccagcac
     4141 ctcgtagatc agcttgtccg cgcgtgcgct cagctcctgg aagcggcgga tgccggacgg
     4201 ccggaacgcg gtgaggatcc gcgccgcgcg cggcgacagg gtgtggacgg gcaccaccga
     4261 cgtcggcttg tcgttgatgt ccatcatgct catcacgacg tcgagcagct cgtccagccg
     4321 cctgtcgggc tcgggcccga agacgatgcg gaacacgacc ttcagggtca gccggtggac
     4381 ccacgggtag agctcgatga cctcgccgcg cggccacgag gcgacgtcgc gctcggcgat
     4441 ggcggcgatg tccgcgctgg cgcgctggac cgtctcgccc tgcatgctgc ggttgacgag
     4501 ccggcgccgc gcgaggtgcc tgtcctcgtc caggcacgcg aggccggtct ggccggtgta
     4561 cttctcgatc gagctcgagc cctcaccagc ccacagaacg tcggcgggcg ccatgaacat
     4621 ctgcttgatc tcgtccgggc tcgcgagcat gacgaacggc gtcggaggga ccctgatgtg
     4681 aagggtgaac ggggtgccgt agcgagcacg gcagcgctcg aagaaggccg tcggacgcga
     4741 ccagatgccg agcgtctgca tcacggagga catgctcggc cccggcggca gcccgctctc
     4801 ggcgttcgac gccttcaccc actcggccac ggtgtcctgg gtcgggcgcg gcgtgggcgc
     4861 cgccatgtgc gggcaggacc cgttgccgtc cgacacggtg gagccgtctg ccttttccgc
     4921 gttcaacatg cccgtagctc accggaccgg cgtcgagccg tcaagtttcg ccccgcgccc
     4981 atcaggcggg ggtcggggtc gaggcgccgg agcggcgcca gtcgcgcccc agcttcttca
     5041 tcatcggcgg gtacacgagc aggtgccgga agggcttgat cgcggccatg taggcgttcc
     5101 cgaatgctcc gttcggcttc acgtagaccg ccatctcgcc gcggtagccg ccgctctcgt
     5161 ccgggaccca gctcaggtgc atcaccccgt ggacggtctt gttgacgatc tccgcggcga
     5221 actcgtcgtc gagctggtag agcgaggtga aggggagcgt ctcgaactcc ggcccggccg
     5281 gtccctcgcg caggtccgcc gggagccggt cgcgcagcgt cggcctcccc gaccgggcgc
     5341 cggcgtcctc gtcgtcccag ccgagcagct cgccgacctt ccagcggatc gaccagagcg
     5401 cgcggccgat gcccgaggag tccttctccg ggtcgcccgc cgccatccgc tccaccaggc
     5461 gcgggaagtc gtccgggccg cccggcgtcg gcagcgccca gacgtcctcg aggcggaagt
     5521 cgcgggtcag ctcgtggatc cgccacggcc gggacgtatg cgcgctgttc ggaagcctca
     5581 tctccggcgt cacgatgcga gcttcgcggc ctcgcctctg tgctcgtact cctcgccgtg
     5641 ctggccgcgc aggtccacga ggcgcgcgtg caggcgctcg agccgcgcgg cgcggtaggc
     5701 ccagatcatc gcctcgtcga ccatgacgac ctcgcgcacc tgctcggacc agtcgacgtt
     5761 cgtccccttg aagcgcgcga cctggcgcgc ctcggccagg cggccgcggg ccgccgcgca
     5821 ctcgcgctcg caggcgagcc gctcacggtc ggtgatctcg atgacccgct cgaggtcctc
     5881 gggctgagcg aggtcgatcc gggcgtgcag cgcctcgcgc agagcgggca ggccgtcggg
     5941 tgacgtccgc agccactcgc ggaagcggtc gatgccctcc tcggtggcct cgtagcgtcc
     6001 ctgggcggag tcgcgcgcgt cctccggcgc cgtcacgcgg cgcacgagac cctgcgcctc
     6061 gaggcgcgga agcgtcatgt gcgccgtgga cggggcgaac cgggcgggcc ggaaccgctc
     6121 cgacaggcgc tgtgccacgc acgaggcatc ctcgggctcc tcgatcacca gcccgagcac
     6181 cgccatgctc gccgacagct caccaacgct tgtgcgcgac actctcagac accacctccg
     6241 accgacaccg gacttcgtcc acgggacaac ggccaatgac accagacgga cgcgctcctt
     6301 acgagttctg cgcagggccg ccaagcgcga tcccgacgct gaccgctcaa gggagctttc
     6361 aatgacgggc ctggattctc ccgacatcgg gatgtcttgc ggcgccgtgg aacttggtcg
     6421 cgggggccga gcctggtact tgacttccgg gcaggtgcct tgaagggtcc gcctatgagc
     6481 cggagccgat tgaccctgcc ctcgatcctg gtcgcgtcgt tcgcggtcag cgtcgacacg
     6541 acgatcgtca acgtctcgct ccccacgctc gtgcgcgagc tggacgcctc cacgagcgag
     6601 ctccagtgga tcgttggcgc ctacagcctc gtcttcgcgg cactgctgct tgccgccggc
     6661 agcctcagcg accgcgtcgg ccgcaagggc gtcctgctgg cgggcctcgc cgtcttcggc
     6721 ctcgcgagcg cggcgggcgg cctcaccgac agccccgagc agctcatcgt cgcgcgcggc
     6781 gcgatgggcc tcggcgcggc gctgatcttc ccggcgacgc tgtcgctgat ctcgaacatc
     6841 ttccccgagc gcgacgagcg ggcccgggcg atcgggctgt ggggggcgac caccggcgcc
     6901 gcggtcgcgc tcggaccgat cgccggcggc tggctgctcg agacgttcga ctggtcggcg
     6961 atcttcttcg cgatggcccc ggtcgccgcg ctcgccgcgc tgctcatcgg gcggaccgta
     7021 ccgacctcgc gcgacccgga cgcgccgcgg gccgaccggc ccgggctggt cctctcggcc
     7081 tcggcgatcg cgctcctgat ctacacgatc atcgaggccc cggactacgg ctggggcgcg
     7141 ggccgcaccg tcgcgggctt cgccgtcgcg gccgtgctgc tcgtcctgtt cgtgctctgg
     7201 cagcggcgcg cgagcgcccc gctggtcgac gtcagcctgt tccgcaacct ccgcttcacc
     7261 gccgcgagcg cctcggtcac ggtcgtcttc ttctcgctct ccgggttcct cttcctgatc
     7321 acgcagtact tccagttctt caagggctac gggccgctgt ccaccggcgt gcgcatgctc
     7381 ccggtggcgc tctcgctcgc gcccatgtcg gtgatcggga ccaagatggc cgtgcgctac
     7441 ggcacgaagg tcgtcgtggc gatcgggctg gtctgcctcg ccgtgttctt cgtctgggtc
     7501 tcgacggact cggagaccac cgggtacttc gtgatcgcca gccagatggt gctgagcggg
     7561 atcggcatgg gcctcagcag cgcgcccgca accgaggcga tcatgggcgt cgtccccaag
     7621 gcgaaggccg gcgtcgggtc ggcggtcaac gacgccacgc ggatcctcgg ggcgacgctc
     7681 ggcgtggccg tgatcggcag cgtctacgcg tcggtctact cgagccggct cgtcgacgcg
     7741 ctcccggcgg gggtgccggg gccccaggcc gacgcggccg aggactcggt cggcgctgcg
     7801 ctcgcactcg ccggccgcgc cgggcccgcg ctcggcgacc gcatccacga ggcagcggcc
     7861 agcgccttct tcgacggcat gagctcgggc ctcctggtcg ccgcgggggt agccgccgtg
     7921 ggggccgtcg tggcggcgtc gctgctgccg gcgcagccgg tggtcagcca cgaggacgtg
     7981 ccggtcgcgc cgccggtccc cgccgtggag taggggtcag ccgcgccgcg cgtgcgcggc
     8041 aagggcatcg gcggtgcggt cgaccagggc gctcgccctc ggcacgaccc ggcccagcag
     8101 caggaagccg tgcatctggt cgtcgacgtg cacgtgctcg acctccacgc cggcctccgc
     8161 cagccgcttg gcgtaggcca cgccctcgtc gcggagggga tcgaactcgg cggtgagcac
     8221 gaacgccggc ggcaggccgg acaggtcctc ggtcgccagc gggacgaggt actgatggtc
     8281 cgcggccggg cccggtgggc ggaagttgcc gaagtaccac tccatcagcg cgcggtcgag
     8341 gatgtagccg gtcccgcgct cgcggtagga gggccagcgg tcgaagtcgt cggcgaccgg
     8401 gtacgcgagc acctggtagg gcggcatcgg ttcgccctgg tcgcgcagga gcagggtgag
     8461 gctcgccgcg gtcgcgcccc cggagctctc gccgaacagc gcgggcggcg ccgcgtcggc
     8521 gccgatcgac tcggcgttgt cgagcagcca gcggtacgcg acgtgggcgt cctccagctg
     8581 cgccgggtgc ttgtgctcgg gagcgcggcg gtagtcgagc gaggcgagca tccacccgga
     8641 gcgcttggcg agccggcggc agaggtcgtc gtgcgtgtcc acgtcgttga ccgtccagcc
     8701 gccgccgtgc aggaacaggg cgaccggaag gcgaccctcg cggggccggt agagccgcag
     8761 gggaagcgct ccggtcggcg ccggcacgga gacgtcctcg acgctgtgca gcgggaacgg
     8821 cgcgcccttc gtgacgaggg ccgcacgcac gcgctcgcgc gcctcctcga ccggcaggga
     8881 gtggaggctc ccctgccccg acgcgatcac gacctcgagg atcatctgcg cgtcgggatc
     8941 gagttcgggg gccatggaac ggccggtagg ctaatggcgc agtgccggat atcgagcagg
     9001 ccgcgctggt ggcgatcagc gacctgcacg tcgaggccac cgacaaccga agcttcgtcg
     9061 agggcctgcg tccgcgccac gacgacgact ggctgatcgc gtgcggcgac gtcggcgaga
     9121 tcatgtcgga cgtcgagtgg gcgctggcca cgctggcgcg caacttcgag aaggtcatct
     9181 ggaccccggg caaccacgag ctgtggacga gccccgccga cgaggtgcag ctgcgcggcg
     9241 acgcgcgcta ccggcacctc gtgagctact gccaggggct cggtatcgcg actcccgagg
     9301 atcctttccc ggtctggtcc ggcgccggcg gacccgtggt ggtcgccccg atcttcaccc
     9361 tctacgacta cagcttcggc gtgaacgtgg cacccaccaa ggaggaggcg ctggctcgcg
     9421 cgcacgacgc cggcgtcgtg tgctcggacg agctcgtgct gcaccccgac ccgtacgagt
     9481 cgcgcgagca gtggtgccgg gcgcgggtcg agtacacgga ggggaggctt acggcgatgg
     9541 ccgccgaggg gctgccgtcc gtgctcgccg gccactttcc cctgctggcc gagctcacga
     9601 gccccctgat gcatcccgag ttcgcccagt ggtgcggcac gctcgccacc gccgactggc
     9661 acacccgctt ccgcgccgag gccgtggtgt acgggcacct gcacatccca cgcaccacca
     9721 cgcacgacgg tgtccgcttc gaggaggtca cgctcggcta ccccctccag cgcaggcgct
     9781 gggtcaaccg ccgtccggtg cctcgcccga tcgttccgtt cgggggcggc gagtgatcga
     9841 gcgcctcgtg ccggagtccg tcgccgtggt ctccacgcgc ggcgacctgc cggacgtgga
     9901 gctgttcgcg gaggaggagc ggtcggtcgg ccgcgccgtg gagaagcgcc ggcgggagtt
     9961 cacgaccgct cgcggctgcg cgcggatggc cctcgagcgg ctcggcgtcg cgccggtggc
    10021 gatccctaac ggcgagcgcg gcgagccggt gtggccgggc gggatcgtgg ggagcatcac
    10081 ccactgcacg ggctaccgcg ccagtgccgt ggccagggtc gaggacgtgc tgtcggtcgg
    10141 catcgacgct gaggtccacg agcggctgcc cgagggtgtg ctggagcaga tcgcgttcgg
    10201 cacggagctc gcgaccgtcg ccggcgacgg cggcggcgtc tgcctcgaca ggctcatgtt
    10261 cagcgcgaag gaggccgtgt acaaggcctg gttcccgctg gccaggcgct ggctcggctt
    10321 cgaggacgtg gagatgacga tcgacgtgcc ggtcagctcg ttccgcgcgc ggctgctcgt
    10381 tcccggaccc gtcgtcggcg gcgtgcggct gaccgagttt cgcgggcgct gggctgtcga
    10441 ggacggcatc gtggccgcgg ccgtggtcgt cttgccgtag cgctcgggcg cgattacgct
    10501 ccggctccgg accgctcgcc gtaccccgga gggaggagga gcagtcgatg gcaccgcacg
    10561 aggtggcccg ttcgagtctc ccggcgtcgg gagacctcgg cgccgacctc atccccttcg
    10621 gcgacgcgct ctccgaccgg ccgcggtcat cgtgcccgga cgccgacgcg gccgtgctgg
    10681 tggtcgaggt cgcgcacgcg gactccctga ccgccggccg ctccgcgctg gagctcggcg
    10741 cgctgctcgc gaagttcttc aggaccgtcg ccgaggtcac cgaagcgcac ggcggctacg
    10801 ccgagcgcga tgacctgtgc gcggtccgct gcgtgttcgg cgatcccgcc tcctccacgt
    10861 gcgcggcgga cgcggcgctc gccgccgcgc gtgagcttcg cgatcgactg gtcggcgagc
    10921 tgccggagct ggcgttcacc atcggcatct cggtgggagc gccgggcgcc gggtggatcc
    10981 gggccaggca ccggttcgag ccgctcgtca tctgcgcgcc cgccagcgag gccaggcggc
    11041 tctgcgggca ggcgcggcgc ggcggcccgc cggtgctcgc gtccgagcgc gcgctggccc
    11101 gcgcctcggc gaccgaggcc tgccgctggt cgtccgcggc ccccgccgcg cactcgattc
    11161 gctgaactcg tgaagtggtt agtggccctg caccaggggt acgcttcgaa ttggggcatg
    11221 agggggttga ctcgcacctg cgtcgtgtgg ggcgcactgg cgctgctcct tgccatgccc
    11281 tcgctcgccc gcgcgggcga cctctcgccg agcagcttcg acccgggtcc ggtcgagtac
    11341 ccgaacgtcg tcaaggagag cgacgtgccg atcgtgatgc gcgacggcgt ccggctctac
    11401 gccgacgtct accgcccggc cgacgcggac ggcaacgcgg cgcccggccg cttcccggtc
    11461 gtgctcaacc agatcctgtt caacaagggc acgacgcggc tggacgggac cccggccggc
    11521 cagatcgagg agggcgccag cggctactcg ccgatcttca ccaagcacgg ctacgtgcag
    11581 gtgatcgtcg acgcgcgcgg caccggctcg tcgcagggcg agttcagcat gttcggtccc
    11641 gacgagcagc gcgacggagt ggagatcgcg cgctggctcg tccgccagcc gttctcggac
    11701 ggccgcttcg ttgcctacgg cgcctcgtgc atggccatct acgggacgtt cctggccgcc
    11761 cagcacccgc ccggtctgaa ggcgatgttc ccgatcgtcc cgatggacga catctaccgc
    11821 gacgtgctgt ggcacggcgg cgggctcgac gtccccttcc tcgcgatctg gcacgggatg
    11881 atcacgtcca cgaagacgcg gccgccgctg tactcggcca gcgacccgga ggccgcgatc
    11941 gaggtgctgc gctcgcgcgt gagcccgagc atcgtgccga cggagatcct caccgagccc
    12001 tacgacaacg actactaccg gacgcgctcg cccggccgcg tcgtgagcca ggtgaacgtg
    12061 cccacgttcg tggtcggcgg ctggtacgac ctgatgcagc gcggaacccc gcggatctac
    12121 aaccagctcc agcttccgcc cgggcagaag cagctcatga tgggcccgtg gtaccacctg
    12181 accggcggcc aggggctcgg gcagcccggt gctccgccgc cgatcgacgt gctcgcgctc
    12241 gcgtggttcg accgctgggt gaagggcgag cggaacggga tcgagaacta cgggccggtg
    12301 acggtcaacc agctgggcgc gaaccgctgg gagacctacc ggcagtaccc gcgtcgcgac
    12361 gtgcagtaca agcgcttcta cctgagcggt gagaagagcg gcacggcgca gtcgctgaac
    12421 gacggctcgc tgagcaccga cgcgccgcgg tcggccgggt ccgacgacat gccgggcaac
    12481 tccgtgaacg gcctctgcac ccgctcgacc gtgcagtggt cggtctcgtt cgtcccaccg
    12541 ggccagccgt gcgagaccga caaccgctcg caggaggcga cgtcgctcac ctacaccacg
    12601 ccgccgctcg ccgacacgat gcacctgtcg ggaccgctct cgctcacgct gaacggctcg
    12661 accacggcgc gcgacacgac ctgggtcgcg accgtcaccg acgtgggccc tgacggaagc
    12721 tcgaaccaga tcacctccgg ctggctgatc cagtcgcgcc gcgcgctcga cgccgcgcgc
    12781 acgaccttcg cgccgaacgg cgacccgatc gtgccgtacc acccgttcac gcgcgagagc
    12841 gtgctgccgg tcgagccggg tgtcagggac tcgatggccg tcgagatctt caacaccgac
    12901 gcggtgctcg agccgggcca ccgcctgcgg gtgacgctca gctccggcga cgtgccgcac
    12961 atcctcgcgc cgcccgacac cgtcctggcc ggcgtgggcg gcatgagcac ggtgcaccgc
    13021 ggcggggacg cccagagctt cctgaccgct ggcctcgcgc cgctcgggcc ggagcccgcc
    13081 cccaagccca ccaagctcca gcgctgccgc tcgaaggcca agcacgcgca caagtcgggc
    13141 aagcgccggc gcgccgcgct ggctcgctgc tcggcgcgct acggcaaccg ggcgacctag
    13201 cggcggggcg accggcatga gcgtctcggc cgagacgatc gcgcagggcc caccgcgcag
    13261 cgccgcgcgg cggttcgcga gcgcggcgtc gctcgacctg ccgatgatcg ccatgctcgc
    13321 cgcgttcgtt gccgagccct cgacgcgcga cttcgcgctg gtcaacctgc tcgtgcaggg
    13381 cgtgctgttc ttgttcggag cctgcctgcc ggcgcacaag acgcgcctga tggctttcgt
    13441 cgacggcatc tggccgtggg ggctggtcgc cctcggcgtg caggcgctgc tcttcggcga
    13501 cacgggctcc ccggtcctgc tcgtcgtcgc tgccatctac ctggccatgg gcctgcgcgg
    13561 cgggctgtgg gcgatggtcg tcgcgttcac cacgcgccgg cgcgaggacc ttccgcgcta
    13621 ccgctaccgg cgcctgctct ggaagcgcga gggctaccgg tcgaagacgg tgccgatgca
    13681 gcacgagatc gtccagcagg ccttctgcaa cgtgtcgttc ctcgcggcgc cggcgctgct
    13741 ggcggtcggc gaccggcacg ccgacatcgg tccgctcgtg gtcgccgggg cggtgctgtg
    13801 ggcggtgagc tgggcgctcg agtcgctggc cgacgtgcag aagtcgcgct tcgccaagcg
    13861 ctcggcggcc gagggcacgg ccggcacctg cgaggacggc ctctggcgct actcgcgcca
    13921 ccccaactac ttcttccagt ggcttcagtg gcacgggctg atcctcgtcg cgctgccgtc
    13981 gctgatcgcg gtcgcggacg acttcgccct gcttccgtgg gtcggcgccg ctgccgcgct
    14041 cgccgcgatc tccgcgggga tgtactacgt cgtcgtgcac tacacgggcg ccatcccggc
    14101 cgagtacttc agcgcgcagc accggcccgg ctaccgggac taccagcggc gggtgaaccg
    14161 cttcgtgccc ggcccgaccc ggggctagcc gagcccctcg aagcgcggct cgatcggcat
    14221 cgccgccgac agcagcggcg aggccgcggc ggggtcgtcc tggtcgcaca cgaacaggaa
    14281 gtcgatgacg cgggcatcga ccgaggcgta gacgccctcg accttccacc tgcggtcgat
    14341 cgtgcgcagg cgcgtgacgt gcccgtcgcc gtcgatcgtc ccgaccaccg agccgcggat
    14401 ctcgccgtcg gccccgtggg ggtcggaccc gggctccgcc gacgcggtga acacgaccag
    14461 gtcgtcggcg atcggcgtcg cgtcgctgaa tgtgagcggc acgccgtcga gatcgccgag
    14521 ctcgtaggcg cgggtgcgca cgtcgccgca gtcgaccgga ccgcccgcgc gcacgccctc
    14581 gacgaactcg ccgagcggta cctccgcgac gatgttgagg ccctgctcgg tgttgccgcg
    14641 gtggaacagc gcgagccggt cgccgagcgc ggcggcgccc tcgatgttca gctccggagc
    14701 gctctcgcgc agccgcgcgt agagcggcgc gaggtcgagc tcgaccggct ccccgctcag
    14761 cgagccgtcg gcggcgaggc cccagacgaa gccgcgctcg cgctggcgct tggtgccgga
    14821 cccgaggccg agcagcgcgc cgtaggggtg ctcgccgaac ggcgggagca ccgtgagcgc
    14881 ctccaggtcg ggcttctccc gcttgcgttc gtcgtggtcg gcgctcagct ccccgcgcag
    14941 gacggtccgc agctctcccg gcgcctcctt cgacatgcgg aagacgccca gcccgagctc
    15001 gtcgtcgccg atcacgtaga cgtagtcgcc gcggcgcgcg acgccgctcg cggcggagat
    15061 gtgcgcagcg cggccgggag tgggggacgc gtcgaggtcc agctcgcgca gcaggctgag
    15121 cgtgagcggg ggcagatcct ctggtgtggc cgaccggctg tccaccgtgc cgtacgttac
    15181 gttcgccgcg atcggcgcgg agtagcatcg cccgtctgat gagcaccgcg gtcgagcccc
    15241 gtacgaccgg ccctcacgac ggcctggtcg gagaggcgcc gccgtcgccg gccggcccgc
    15301 tcgagaacgg cgacggcgcg ggcctcggtt cgcccccgac gatcgcgctc ccgcgactcg
    15361 tccaggccct gcgcttcaac cagcgccaga tccagttcgt gttcggcgcc cggcgcaggc
    15421 acggcgaggt gttccggatg accgggctgc tgccgaacgg gccggtgatc accgaccacc
    15481 ccgaccacgt gcggtcgctg ttcacggcga agccggagca ggctccgtcg ctcaccgggg
    15541 agtcgtccct gcggccgatc gtcggctcga actcggtgct cacggccctc ggcgagcgcc
    15601 acatgcgcca gcgcaagctg ctgctgccct cgttccacgg ggaggcgatc gagcgctacg
    15661 tccagatgat ttccgacgcg accgagcgcg agatggcgga ctggccggtg gaccggccgt
    15721 tcgccctggt gccacggatg caggcgatca cgctcgacgt gatcatgggc gggatcttcg
    15781 gcatcgaggg gcggcccgag agcggcactc ccgagcatgg gctgcgcgcc acgacgcgga
    15841 ccctcgtcgc ggggtcgacg aatcccgtcg cgcagctcgg cgagctgatc aacaccggcc
    15901 gtgacgaggc ggtcgggccg ctccgctggg cgctcgcggt cctcgaccgc caggtgtacg
    15961 cggtgatcgc ggcgcggcgg cgctccgcgg acctcgagga gcgcggcgac atcctctcgc
    16021 tgctgctcca ggcaaggacc gaggacggcg aggcgctgac cgacgaggag ctgcgcaacg
    16081 agctgctcgt gctcgtactc gccgggcacg agaccaccgc caaccagctc gcgtggacgt
    16141 gggagcggct ggtgcgcacg cccgccgcgt acgaccggct gcgcgaggct gtgcgctcgg
    16201 gcgtggacgc cgaggagcac gtcgaggcgg cgatcgtcga ggggatgcgt gtgcggccgg
    16261 tggtgccgat gatcggccgc cgcgtggcgg tgccgtggcg cttcggtccc tacgcggtgg
    16321 cgccgggcac gcccgtgggc atgagcatcc tgctgctgca ccaccgcgac gacctctatc
    16381 ccgacccgtt cgcctaccgg ccggagcgct ggctcggtcg caagcccggc acatacgagt
    16441 ggatcccgtt cggcggcggc atccgccgct gcctcggcgc ggcgctggcg atggccgagc
    16501 agcgcgtggt gctcgaggcc atggtccgca cgctcgacct cgaggccgcc gaccccgagc
    16561 ccgagcgggc ggcccaccgg aacgtgacga tgatcccggc gcgcggcgcg cgcgtcgtgg
    16621 tgcgcgcgcg gcgcggctga gcgcaccggc agaagtttct gcagcgcttc gcggttctcg
    16681 gttagggtgg cgcaactgca ccccatcccg cgagagggag gttcggaatg aaccaggaca
    16741 agccggctga cggacgcaag gcctggcagg caccggagat cgtcgaggtc ggcggcgtcg
    16801 agaccgtcac cacgtacgac gacggcaacc tgcgcgacaa ccagggcgac gcgaccccca
    16861 agtacaaggt cttcaccagg accaagacgg agagcccgga gtccgagctg ccgcacggcg
    16921 actagcggga ctggacgctg accggcgagt tcgccctgcg gccgtcggtg cgctacgcgc
    16981 gcgtcgacgg gctgggcgtg ttcctcgacc tgcggacctc gacctacaag gtgctggacg
    17041 aggtggcgac gtcgatgctg gatgcgctga ccggcgcggc cgatcccgag gccgagctcg
    17101 ccggctggtg ccgcgagtac gagatcgacc cggagaccgc gcgggcggag ctggcgcgct
    17161 tcgccgacac gtgcgtctcc gacgggctgc tgggcgcgcg cggccgcgac gacggcgagc
    17221 cggcgccgcc gagcgctgag ccgctcgctt ccgcaccgag gggcccgcgg gtcgggcggg
    17281 ccgtgcacgc gctggcggcg acgtggcgga gcctgcgcgc ggacgggttc ggccccacgt
    17341 acgagcgcta cgcgcgcatc ccggtcgcgc cggccgcatc cgcgcccggc gcatgcgtcc
    17401 gggcgttcgt gcgcgcggag aacgtctggc cctcgaagcg cgcgccggac gactgcctgc
    17461 tgcgctcgct cgcgctgttc cgctacctgc gcggcgaggg ggtcgccgcc gagcacgtga
    17521 tcggcgtgtg gcgcttcccg ttcctcgcgc acgcgtgggt cgagtgcggc ggcgcgccca
    17581 tgctcgacga gcgggtcagc ggcttcaccc cgcttgcgcg catcgccgca gccgacgccg
    17641 ccgcgcggtg acgagcgcgc acttctcggg ggcgctcagc gccccgaatc ggctcgccgc
    17701 gctcgacggc gaggcgatcg gcggcccgcc gtccgccggc tgccgatgcc tgttccgcgg
    17761 ctgggtcgcc gacgcgcccg cgctgcgccg ccggctcggg ctcgccgacg agggcggtgc
    17821 ggagccgggc ggcgccgagc gcctgctggc ggcggcgttc cgcgagtggg gggaccgcct
    17881 ccaggcgcgg gtggacggcc agttcgcggc cgtcgtgctc gacgccgccg cgggcacggc
    17941 gctgctgacc cacgacgcgc tcggcatcgc tccgctgtac tggcgcgagc gcagcgggcg
    18001 gctggagttc tccacgcgcc tgcgcgacct cgtcgacgcg cgtgcggctg ccgacctcga
    18061 cgacgagtac ctggccgact tcctcgcgtt cggcgttccg tcgagccggc ggacgccata
    18121 ccgcgccatt cggcggctca tgccgggcac gagcctgcgg tggagcggcg ggcgagtgac
    18181 cgagctgcgg acctggagcg cgtccgacgt cgagccggtg cgcttccggg acaaccgcga
    18241 gtacgaggag cgcttcgagg agctcgtggg ctcggctgtg ggtggcgcgc tcgacaccgg
    18301 ccggccgacg tggatcgcgc tgtccggcgg aaccgattcc gtgacggtcg ccggcgcggc
    18361 ggcacgccac ggaggcggcg acctgcgtgc gtactcggtc gtcgcgccgc gctggcagga
    18421 gtcggacgag tcgcgctgga tccgcaagac cgtggacgcg ctcggcctgc cctggcagcc
    18481 gatcgatgcc gaggacgtgc tcccgttcgc gcgtatgccc acggagttct gcggagagcc
    18541 gaaccagtcg atcgtcaacc tggggctgat cgacacggtc caggacgtgc tcggagcgag
    18601 cggctcgcgc gtgctgctga cgggcaaggg cggcgacggg ttcgccggcg cctcgtcggg
    18661 cgacgtgccc gcgcacctcg ccgacgcgct cttcgaggcg cgcccgctga ccgccctgcg
    18721 cgagacgctc gcgtggaagg cggcggccga cgacgtgcgc tcgctgtcgt tctggctgcg
    18781 ccgcgggctg atcgctccgt cgctgcggca cctgggcggg cgccgcgcgc gcgtgcacac
    18841 gcagcggctg accgcgccgt ggctgcggcc cgactacgag cgcacgttgc gcgtccgcgc
    18901 acgcgcacag cgttcgccgt cgccgcgctg ccgcacgccg gggcggcaga tgctgctgga
    18961 cgagctgtgg ctgtgcagcg cgcagatgga gcccgccgcg acgatcggcc acgaggtgcg
    19021 ccacccgctg ttccaccggc cgctgttcga gttccagtgg gcgatcccgt ggcagcagaa
    19081 gctgctgccg cgctgcgacc gctacctcca gcgccgggcg ctgcgcgggg tggtgcccga
    19141 ctcggtccgc cggcgggtga gcagtgccac cggcacgcgc gcgctggtgg aggggctgcg
    19201 gcgctcgcgc gagtggcagg actacctgtg cgagcgcccg cgcatcgcgg agcacgggat
    19261 cgcggacgcg gaacgctggc ggacggcgat caagcaggcg agcgtgggac agacgcacgg
    19321 ggaccatctg ctggtcgcgg gcatcgcggt ggaggtctgg ctcaagcagc tcgacgagca
    19381 cggagggggt gccggatgag ggaggggctc acgagcggcg cgcgcgtccg ctgggaggac
    19441 ggcgagctgg agctgcgccc gcgggagcgg ttcctgccgc tgctgcgtgc cggcgtcgag
    19501 gccgctccgg accggccgga gccgaggcgc cggctcgctc ggctgctgct tcagaccgag
    19561 cagctggagg agctggagga gctggccacc ccggcgctcg acgatcccgg cgcggacccg
    19621 gaggtgctgc actgcctggg gctcggagcg ctggctacgg agcggcccga gctggccctg
    19681 cgcgcgctgc ggcgggccgt ggacctgggg ttcacgcaca gcctgggcgc gctcgcgaac
    19741 gtgctgcgcg acctcgggcg cgacgacgag gcgctcacgg ccgcgctgca agcgctcgag
    19801 ctggatccct cgaaccactc cgcgagcggc gttgtcgccc gcatcctgct cgagcgcggc
    19861 gagcgcgagc gcctgtggtc gctgtgtcag gagatccggc ggggcggagg ctggggcgcc
    19921 tacctcccgt ccgcgatggc cgtgtgcgcg cgcaccggcg agcagcaccg cgaggtggag
    19981 cggctcgtcg gccgcgaggc gtggttcctg cgccgcgacc tgggcctggc gcccgagttc
    20041 agccgccgcc tcgccgagga gctgctcgac ctgatgggca ccggagacct gccgcggcgc
    20101 aaggcgagca ccggcaccgg ccggcgcatc gaccagcttc aggtggtggc gcggacgaac
    20161 cccgttgtcg cggagctgtt cgcggctctg cgcgcggcga tcgaggagta tctcgcggag
    20221 cgcgaggacg cgctcgcgga cgagctgctg ggccagcagc tcggagggcg gctgccggac
    20281 gagctgtcgc tcggggcctg ggcgctcgcc atgcgcggcg acggccacga ggagtggcac
    20341 atgcacccga gcggctggct gagcggcgtc tactacgtcg aggtacccga catgacggcc
    20401 agcgccgcgg agcatccggg cgcgatcgat ttcggcccgt tcccgttcac gggcgtcgag
    20461 gaggtgccgt cgtggccccc ctggcgcatc gcgccgcgga cgggcgagat gctgctgttc
    20521 ccgtcgtcgt acgcgcaccg cacctggccg accggggtgg acgaggaccg gatcgtcgtc
    20581 gccttcgacg tgctcgcggc gcaccccgca gaggcgtgat ggcgagcgcc gagctcgacc
    20641 cggcggcgcc gatcgcgcgc agccccgagg cggtcttcgc accgggcccg ccggagaact
    20701 tcatcatgca cctgacgagc ggccgctgcg tgagcgtgga cgacgccggc gtgcgcatct
    20761 gggagctgct cgagtcgccg cgttcgctgt cggagctggt cgatcgcctc gcgcaggagt
    20821 tctccggcga ccgcgacgcg atcgccgccg acgtgcgcgc gttcgtcctc gccctgcgcg
    20881 actgcgagct ggctctaccc tcgccgcaat gaccggtccc ggatcagagg cgatcacggc
    20941 cgaggggctg cgcgccctcg aggccgagct ccacgagctc gagacagcgg ggcgcctggc
    21001 gatgtcggag cgcatcaagg cggcgcgcga gctcggcgac ctgaaggaga acgccgagta
    21061 ccacatcgcc aaggacgatc aggcgcacct cgagaccaag atcaagcggc tgcgccagcg
    21121 cctgctcgcg gcaaaggtgg tcgaggccga cgcggacgac gccacgttcg cgttcgggcg
    21181 cacggccaag gtgctcgacg aggcgaccgg cacggtccac gagtggacga tcgtcggccc
    21241 gaccgaggcc gacatcaagg ccgggaagct gtcctcggag tcgccggtgg cccaggctct
    21301 gctcgggcgc gcgcccggcg acacggtcgg cgtcgagacg ccgcgcggca cccgcagcta
    21361 cgtgatcaag aagctcgtcg ggtagggcgc gtcagacgga cgcgtgctgc gcgaccgccg
    21421 cggccggtgc ccgggcgggc gtggcgaagc cgcggcgcac cggcgcgatc ggcgcccgca
    21481 ggtcggcgtg ctgcatgcgc tcctcgcgca tcgtgcgctt gtactcggag ggcctcacgc
    21541 cgacgtggcg cttgaatgcc ttggtgaact cggccttcga catgtagccg acccgctcgg
    21601 cgacgacgta cacgggcagc gacccctccg cgagcagcgc ccgcgcgcgt cgtatccgcg
    21661 ctacggcgac ggcctgccgg aaggtgatgc cctcgtcctc gaaggctcgc tggagggagc
    21721 ggcgcgaggc cgcgatctcc cgagcgacgt cgtcgagtgc cagcgggcgg gcggggtcga
    21781 tctccaccac gcggagggcg tcgtatacga ggccgcgccg ccgtgctctt gtctgatcgc
    21841 gcatgccacc gatacgggtc gcccgcgcgg gcggatcacc gcgtggacct tcggtcggcg
    21901 cgtcgccgct agcaggcgcg gaaggtccgc gcgctgcgca gcgtcttgcc gcccaccgtc
    21961 ttcacggtga cctgcacgcg gtaggcgccg cgcttcggca tccgctcgag cctgaccttg
    22021 gcgggcaggg cggcgccgcg cagcttcctc acggtcttcc cgttcacggt gaccaccgcc
    22081 gaggccgccc ggttcgaccc cgcgtggcgg acgatgcgga acgcgaccgc gcgccgcttc
    22141 acgcagcgct tgttcgtgga gaacgaggcg gcggtgagcg cccgcgcccg cgcgccgctg
    22201 cccgcgggct gagccgccgg ggcgccggcg gggggcgcga acggggtcgt ccccgacggg
    22261 gcgcttccga ccggtcccgc ctgcgcgccg agccgcggga tcgtgagggt gctcggcgcc
    22321 gccgggtcgg ccaccacggt gaccgggccg gcgaggttgt tgccgtggtt ggtcgagttg
    22381 ctcgtggcga tcacgacgcg catcgaatgg cccttggcga agcggtgggc gatgcccggc
    22441 agctcgatct cgacgggctt ggtcacgtcc gcgacgcgca ccgccgagag ctggttgcgc
    22501 ggcagcacct ccgccccgct cgcgtccacg tcgagcagct tcacgaacag cacgagcttg
    22561 ctggccgggc cctgcgcttg cgactccgcg aacgtcggcg cgtcgagctt cacggtgagc
    22621 ctgggcacgc cgaccacgtc gacgtcctcg gtgagcggag cggtggtgta gctcgcggtg
    22681 gtgccggccg cgttgccggc cgggggctga gcgatcgcgg cgccgcccgt ccccgtggcg
    22741 cccggcgccg cggcgaagct cgcgctgccc ggcttcacgc ccgccctgtc gggcacgagc
    22801 tggtcggtgc ccgagaggaa cacggtctcg tccctgctcg ccggatagcc gcgcgtgttg
    22861 cccaccgcgg gggcggcgtc gccctcgtac ttcacccagt cgcggaggaa cgagaagtcg
    22921 agcgtcggcg ggtcgccgat gccgcgcagg tagtagtcga accactcgag cgccatgcgg
    22981 ctctcgtacg cggactcgag gttcgtggag ttgctctcac cgccgatgtt gccgcccgag
    23041 tgccccgacg agcgccagag catcttcacc ggcgtgccct gtgcgcgcag cgcctggtag
    23101 gtggcgacgg cctcctggat gtcgaagagg gtgtcgctct gaccctgcga gaggaacgtc
    23161 gggatcttga tcttcgacat gtaggtggcc accgaggcgt ggcgcagcag cgcaagcgtc
    23221 gcctcgtccg cgtacccggt ggaggcggac gtgacgagcg acgtgcagac ctggtcagtg
    23281 aagttcgggc aggcgccgag gtggctcggg tcctggttct gcaccgcctg ctggaagccg
    23341 acgctcaacc cgagtgcggt gaacagcgcc ggccagtcgg acttggtcac tcccggcgtc
    23401 cggtaggtca cgccgcgctc gaagtcggtg ttgttcggga cgagcgagta cgacaggtcg
    23461 ttccacgtga tcgaggggac gatcgtgtcg aggcgcttgt cgatgcccgc gatggcgaac
    23521 tggatctgac cgccgtagga gccgccgatc atgcccgcgc gcggatcgcc gggggcgtcg
    23581 gcgcggacga agtcgatctt cgtcccgtcg ttggcggcct tggtcccggc caggaacgac
    23641 acgagctggc tgcccgcctt gccgtcccag tcggggtcgt cgagggtgac cttgcagccg
    23701 ctgttgccga agccgaggcc cgagtacgcg aggaacacgt agccgcgctt ggcgtaggcg
    23761 agcccgagcg cgtcgtagtc ggacttgcct ccgccgaacc cgttcgtggc catgaccgcg
    23821 gcgacgggat cgtccttgga cgcgcccgcc ggcgtgaaga ggttcgccgt gaccgtgcac
    23881 tccgtgtcgt cctgcggccc gacgaccaca tcgaaggtga gcgccttctt ggtgggctcg
    23941 gcggcgaggg cgggagaggc ggtggcggcg agcgcggtga gagcgaccgc ggcaagcatg
    24001 agcatccggt tccccctcat cccaccgaga cgatagagac aaaacgcgac ctgcgcgccg
    24061 aagcgagcgc aggtatcttg cacggtcaaa tcacccggga ccggggaggg cacgcagatg
    24121 cttcgacgag cagacgaagt gcgcatggag cgcaagccgg ggagggcgaa ggggaagggc
    24181 gaattcgagt gccgatacct cgtggaggag ggcgagaacg agaacggcat ccagttcgtg
    24241 cacgacgaca ccctcgaccc aggcgcctcg ttcggcaccc accggcacat cgactccgag
    24301 gagatctacc tggtcctcga cggcaccggg caggcgtggg tggacgacga ccgctgggac
    24361 gtgggtcccg gtgacgtggt cacgctccac aagggccagt cgcacggcat cgaggcgaac
    24421 gcggatggga agctgcgctt cctggcgatc ggcatcggcg ggccgaagcg gaagtacgag
    24481 gttccctgag cgcgccgtcg cggcctgacg tggggcgccc agcggcgcgc tccgcggacg
    24541 ccggagccgc gcgccaccac ttcgccgtcg acgtcctgcg cgacgcgccg ggcgatcccg
    24601 ccgcggccgt cgaggcgctg gtcgaggcgg tcgcgagccg gctcggcccg cccggcgccg
    24661 acgcgctcgc acgggtcggg ctgttcctgc ccgccccgat ccggttcgag gacgcggagc
    24721 cgatcgttgc gcgcgtgctc ggcgagccgt gcccgccgct cgagctgttc gtgcagccgg
    24781 tcgtgggcgg cggcgccgcg gcgctgacgt ggtccgtgac cggcgacggc gtgcgggtcg
    24841 agcgcgcgga gccgagtgcg gcacgcgtgg tggcgcccga ggtagagctc gccgtgctgg
    24901 ggcacgcggc cggcgagccc ccggccgcga ccgccggaga cttcgccgcc ggcctgcgcc
    24961 gcttcgatac gcgcctccag ggcctcggac tgcgcttcgc cgacgcgatc aagctctgga
    25021 ccgtgttccc ctcgacgccc gagctcggca ccgagcaggc gttcaacgag ttcaaccgcg
    25081 ggcgggtgga ggtgttcgac gcgctcgact tccacctcag cgagggcacc ggcgccccgc
    25141 gctacccggc cagcacgggc gtgggagcgc tcgacgcgac ggcgccgctc agcggcgtgg
    25201 cgctcgccgg cggcgcccgc ggtgtgcagg tcgagaaccc cgcccaggtc tcggccttcc
    25261 gctacccgac cgagcggatc gacaagccgg cgctgttctc gcgggcgatc gccgtggacc
    25321 tcggcacgca cgtgatgacc tacgtcgccg gcacggccag caccgtgggg gcgaagacgg
    25381 cgcacgtggg cgacccggcc gcccaggccg agcagaccct caacaacatc gacacgctga
    25441 taacggcgga gaacctcgcg cgctcgggca ccccggtaac cgagggcggg ctcgcgacgc
    25501 tcagtcacat cgtcgtgtac gcggccaccg acgaggccga ggccgcggcc gtcgaagtgt
    25561 gcgcccggat ggtcccgccc gaggtgccga tgctgatggt gcgctcgcgc ctcacgcgtg
    25621 acgagctgtt gttcgaggcc gacggcgtgg cgttcgcgcc gagcgctgcg cccgccgact
    25681 agccagttcc cgacccctcg tgcacgacct cgccgggcag ctcgccccgg acgatccgct
    25741 gccacatcac ccggcggcgc ggcttcccgc tcgaggtgcg ctcgatcgag cccggatcgc
    25801 ccgagagcac cagcacgcgc gacgaggcgt cgccctcgcg ctcgagcatc gccgccgcct
    25861 tctccaccca cgggccggcc ggcgcctccg ccagcgcggc gatcaggttc gccccggcgt
    25921 gcgtcccggc gagcaccacg cacttgccct tcggcacgcc gagcgccgcg atgcgcgcct
    25981 cgacgtcctc cacgaacacg gtgcgcccgc gcaccttgac gctgtcgccg agacgaccca
    26041 tcacgtagag ctcgccctcg tacacgaagc cggcgtcgcc gctcagcagc ctgccgttct
    26101 cgaagcgggt cgatgcgccc ggctcgccga ggtagtcgtc ggcgaccgtc gggccggtca
    26161 ccgcgatctc gccgagcgcc ccctcgggca ggtcgcggcc gtcctcgtcc acgatcgcca
    26221 cgcccacctc cggatgcggt ggcccgcagc cgacgagcca gccctcgccg gacccgatcc
    26281 gctccgcgtc ggtgagcatg gcgctcgcca gcacgggcag cggctcgccg aagcgcgcgt
    26341 cgtcccagtc ggggcgcacc gcgcgcggca cgtcctcgag cggcacgccg gtgacggcga
    26401 gcgtggcctc ggccaggccg tacgcgggca ggaacgtgga cgcgcggaag ccgcgcggct
    26461 cgagccggcg ggcgaagcgg ccgagcacgt ccgcgtcgag ccgctcggcg cccacgatcg
    26521 ccacgcgcca ctgcgagaag tccatcggct cgagctgctc gtcgctgagt cgcttgttca
    26581 cgtagccgaa gccgaagttc ggggccacgc cgagctgcgc gccgtgcacg ccgtagcact
    26641 cgagccagcg cacgggctcg cggatgaact ggtccgggcg catcacccag acgtcgctct
    26701 ggttgaccat cggtgtgacc atgcagccga tcaggcccat gtcgtggtag agcggcagcc
    26761 aggtggcggt cgcgtcctcg gggcgcatcc cgagccagcg gcggatcatg gccacgttcg
    26821 cctcgaggtt ctcccacgtg acccgcacgg cgcgcgggcg gcccgacgag ccggacgtga
    26881 actggagcag cgccagctcg gcgcggtcgc ggcccggcgg cgcgggcgcg ggcccctcgc
    26941 tcgcctcgat gcgcgccacc cgcccgggca ccccggccag ctcggcggcc tcctcgatca
    27001 gctcctcgaa gcccgcctcg accagcacga gcacgggctc ggccgcgcgc aggatcgacg
    27061 ccgcgtgctc gacgtactgc gatcggtcgc gcacgaacgg cggcagcgcc agcgggctcg
    27121 gcgtgttgcc ggcggccagc gcgcccacga acgcggcgat gaactcggga ccgctgcggg
    27181 tcacgatcga caccacgccc gtgcgtgcgt ggcgcagctc ggcgatctgc tccgcggtgc
    27241 cgcccgcgag cgccgccagg cgcgggtact cccacatcgc ccagccgcct ccgtcctcgg
    27301 cgaagcgcag tccgtgtccc gcgcggggct cctcgagcca gtcgagcagc ccggttcgcg
    27361 ccaccgtctc ggtgcccccc ggccgggcgt tggtcgagct cgtggacacg gcggcgcgcg
    27421 accttaccag cagtagagtg cggctcgaaa tggccggagt ccaccgcggc gcgacctacg
    27481 cggacctcat tctcgcggcg cgccggcgct acccggacga gcctgtggac cgcaccggcc
    27541 agctcgtcga ggcggtgatc ggcgcggggg tggagcgcgg cgagggggtc gcgctcctgg
    27601 ccaccggcaa cccgcacgcg agcgcggcca cggccgccgc gctggcggca ggagcgcgcg
    27661 tcagcgccct gcatccgctc gcctccgccg acgaccaggc gttcgtgctc gacgacgccg
    27721 agatctcgac actcgtctac gacccggtcc acctcggcga ccgcgcggcg gacctcgcga
    27781 gtcgcgtgcc gcgggtcgag cggttgctcg cgctcggccc gtccgccgac gcgcccgacc
    27841 tgatcgagct cgccgaccgg ctccccgcgt gccggccgcg ctcgctcgcc gaggagggcg
    27901 acctcgcctt cgtccactac accggcggca ccaccggccg gcccaagggc gtggcggtct
    27961 cgcacgccgc gatggtccac ggcgcgctca tggtgctggc cgagtgggac tggccggccg
    28021 aggtccgctt cctcgcgtgc acgcagatgg cccaggtgct gctcgtcccc gtccgcctgc
    28081 tcggcgggtc ggtccactac tcgatgttcg atcccgagct ggtgctgcgg acgatcgagc
    28141 gcgagcgcat cacctgcatg tacctcgtgc cgtcgatgat ctacgcgctc ctcgaccacc
    28201 ccgggatcgg cgacgccgac ctgtccagcg tggagtcgat cttctacggc gcttccacga
    28261 tcgcaccggc gcggctcgag gaggcgctcg agacgttcgg gccggtgatg tgccagatct
    28321 acgcctccac cgaggcgacg cacacggtga ccgtgctgcc gaaacgcgac cacgacccgg
    28381 cggttccggg ccgcctcgag tcgtgcggcc ggccggccgc cgcgctcgac gtcgaggtgc
    28441 acgacgagga cggccgtgcg ctggccccgg gtgaggtggg agaggtggcg gtgcgcgggc
    28501 gctcgctgat ggagggctac tggcgccgtc ccgagttgac cgcggccgcg ctgcgcggcg
    28561 ggtggtaccg caccggcgac gtcgccttcc aggacgagcg cgggttcgtg acgatcatcg
    28621 accgcagggg cgacgcgatc atccgcaacg gcttcaacgt ctacccgcgc gaggtcgaat
    28681 cggtgctggc gagccatccc gccgtgctcc acgcggcggt cttcggggtg cccgacggca
    28741 ccgccggcga ggaggtcatg gccgcggtgg tcccgcgtgc gggcgcgcac gtgtcggtgg
    28801 acgatctcgc gcggctggtg cacgagcgca agggcgtgcg ctacgtgccg ggggcgatcg
    28861 aggtggtcgg cgcgatcgcc gcgacgccga gcgggaagcc cgaccgcagg cggttgcgcg
    28921 agctggcggc ggcggcgaag cccgagcggg cggcggcggc agagcccgag caggcagagc
    28981 ccgagcgggc ggcggcggcc gcccggcccg agcccgccta ggccggtccg cgcgcggcgc
    29041 gcaccacgcc ggcgagctcc gcgaccgtgg acgcctcggc gaactcctcc agcgggatct
    29101 ccaccccggc gcggcgctcg agctcctcga ggatctggag cgccagcagc gactcgccac
    29161 cgatgtccac gaactcgtcg aacgcgccca ccgacggcac cttcagcacc tcccgccaga
    29221 ggtcggccac ctcctgctcg agctcgtcgc gcggcgcgac gaagacgcgc tcgcccgagc
    29281 tggtcgcggt ggcgggatcg ggcagggcgg cgcggtccac cttcccgcgc gcgccgatcg
    29341 ggaaggcgcc gacgccgatg aacagcgacg ggaccatgta cgccggcacg cgctcgcgca
    29401 ggaagtcgcg cagcgtggcg gtctccggca cgcggtcgcc gtcgcacacc aggtaggcga
    29461 ccagccgccg gtcgccctcg tccggctccc acatggtcac gaggcactgc gcgatcccgg
    29521 ggaaggcgtt cagcgcggcc tcgacctcgc cgggctcgat ccggaagccg cgcaccttca
    29581 cgaggctgtc ggcgcggccg gcgtactcga gcgcgtcgcc ggcccggcgc accaggtcgc
    29641 gcgtgcgcag ccagccctcg ccgccgcccc cgggctgggc cccgaagcgc tcggcggtga
    29701 gctccggcgc gttcaggtag ccgctcgcgg gggccacgcc gccgacgtag agctcggccg
    29761 atccgtcgcc gttcgcgggc gcgccggtgt cggggtcggc gaacgcgatc tccacgccgg
    29821 ggatcgggtc gcccatcgga gcgacgggcc gctcgtcctc gcgcgtgcag cgccagtgca
    29881 tgacgagcgg cgcctcgctc agcgcgtaga ggttgtgaat ctccgcggcc gggagccggt
    29941 cgaacgccgt gagctggagc gacttcgtca gcggctcgcc gccgcacagc acgtgcctaa
    30001 ccgcgcccat gtcgcgcggg tcggcctgcg cgaggaagtg cgcgaagctc gacgggatga
    30061 agcccaggat cgagacgcgc tcgtcgcgca cgatctccgc gacggcgcgc aggtcgcgct
    30121 cacgcccggc cggaaggatc gccgcgtagc cgccggtcac gttcggccac acgagctggc
    30181 gcaccacgct cacgaagctc acggtggtct tcaggaaatg gcggtcctcg ggggtgaagt
    30241 cgtaggactc cgcgacccac gggatgaacg ccgagcaggt ggcgtgcgag agcatcacgc
    30301 ccttgggctc gcccgtggag cccgacgtgt agaagacgta ggcgaggtcc ccgggctccc
    30361 cgggcgccgg atcgacccgg tcgggctggc gcgtcagctc gggggagtcc cagcgcaggt
    30421 cgagcacccg catgccgagc tcgccggcgg ccccgggcac ctgctcggcg cgcgcgaccg
    30481 cgaccgtcgc gccggccagg cgcagcgtcg ccgcgacgcg cgaccacggg tcggtcgggt
    30541 cgagcggcag gcacgtggcg ccggccttca gcacgcccca catcgcggcg gcgtagtcgg
    30601 acgagcgctc gacgagcagc gcgacgagat cgccgctgcc cacgccgtgc tcggcgagcc
    30661 agccggccac ccggctcgcg cggcggtcga gctcctcgta ggtccagacg tcgtcgccga
    30721 gcccgacggc ggggcggccg gcgtgccgct cgaccgcgcc ctcgaacagg tgctggagga
    30781 cggccggctc ggcgctcaat ctgtgtcgtg cctgcgcgcg cggatcgcca ggtcgggaag
    30841 ccgcgactcg ccgtcgcagc tgtgcccggc cacctcgccg tcgggggtgg tgttgcaagg
    30901 cacgacgcgg ttcaggatcg agccgcggca gtactcgagg aagtcgccgc tgtcgtgccc
    30961 gcccagcgcc gcgatcatcg cgagcgtcgg gtcgaggctc ttccagcggc cgtcggagtc
    31021 gcgcacgcgc acccagcggt gcccgccgaa gccgaacagt ccgagcagga agcccgactg
    31081 cggctcggcc tcgagccctg cggctcggca gtcgcgtacg agcacccccg tggcggcgat
    31141 gcaatcggcc gcgcggatcc gctcgaccga gtcgaggtcc gtgcgcagcg cgggcggcag
    31201 gacctggaag cggtagtcct cgacgaccgc catgaacgcg tcgcgcacct cgtccgacac
    31261 gaggcgctcg accgacccgc gcgtggcgat ggtcgccgac agggaggcgg agccgggctc
    31321 gccggaccac tcgtacacgt ccgcgtccgg gtcggagccg ccgggcggcg cgctctcggc
    31381 cagctcgccg ccgagctcct ccggcacggg ccggccgaac gcccacgccg ggtcggcccc
    31441 gcagccctcg cggcgcgggc agcggatctg cgccccgata cgccaggtgc gcggctcgct
    31501 ccaggcctgc tcgccctcgc gtccgaggcg ccccaggaac gccaccccga gctcgggcag
    31561 cgagcgcccc gagcccgagt gcagcgcgag gttgtagacg tcgcggcgat cgaagcgctg
    31621 tgcctcgccc tcaccgctcg acgggaagcc ctccgccacc acgcggtcga gcacctcctc
    31681 cgggcagcgc aggacctcga gcgcggccgc ccggtccatc gactcgtcgc ggtagggctc
    31741 gggaatcggc acgagctcgc gcagcgagcg cagccagtcg cccgcagggc ggcgcgtgtc
    31801 gccggcgacg gccatcagtg gcggtgcacc gaggttgcgg ccgcgacccg gccgcgatcg
    31861 gccgacgaca ccgtgcagga cccgaaggtg aagttgcgcc cgcgctgggc gagcgtcgcg
    31921 tccgcctgga gcgggccctc gccggccggt gccttgcgca ggaagtcgac ccgcagctcc
    31981 acggtcgcgc gctcgccgga cgcggggagc atggaggcca ccgcgccgtc gagtgccgcc
    32041 tcggccagca gcgccagcgc accgccctgc acctggcgca gcaacg
//
LOCUS       DASVPL010000189         4398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_258619, whole genome shotgun sequence.
ACCESSION   DASVPL010000189 DASVPL010000000
VERSION     DASVPL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258619"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF517_09870"
     CDS             complement(<1..318)
                     /locus_tag="VF517_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8053290.1"
                     /translation="MNTESTATQGDGPGGDDLRAGLRQMWNAVAPAWGEHAEYIDELA
                     SGMTGRMLDLTEPRPGEQVLELACGTGGLGMAAAERVAPGGEVVMSDVAAGMTAIARE
                     RADA"
     gene            387..1064
                     /locus_tag="VF517_09875"
     CDS             387..1064
                     /locus_tag="VF517_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8053291.1"
                     /translation="MAGPVKQRPYDSPRRRAQAADTRRAMLLAAERLFERDGFARTTI
                     AAIAAEAGVAEKTVYLGFESKSGLLRALWNLRLRGDEAEAAVAQREWYREMLDEPDPA
                     RKLRLTARNARVVKERIGPLFGVIRDGAPTDTDVAALWERIESDFYDNQLAIVKTLAA
                     MDALHPGLDAARGADILWTLNHPDVWRALVSRRGWSADQWEEWFAQTAAAQLLATKPV
                     GRRRRGE"
     gene            1075..2487
                     /locus_tag="VF517_09880"
     CDS             1075..2487
                     /locus_tag="VF517_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053292.1"
                     /translation="MGRLAAVLAAGLIALAGATAGAHAQTLEDTCASAELVPGACVGI
                     SKITERAAAECRRAGVPDESCIAPMARRVMREEVRAHASSWLHRTLDFQYELGSRVPF
                     RDAPWIGTHNSFNSTSEFPTVSHTDSNQQLSLTHQLRIDVRSLELDVHWLPSPHAAGQ
                     PAPVVCHGRGADELHAGCTTERLLSDTLTEITTWLRDHGDQVILLYIEDAVDAPEGYD
                     PTTAVLDQGLRDERGRSLVYRPPAGGACTDLPLDLTREKVRAAGAQVVIVSGCGSGAA
                     WHGLVFDWNDVHVEGGNDGYRDAPVCDQDPDGDGRPQFNRETYATKLVRFFEDSTWLS
                     ATLTGEGEGLTPEIARRMSRCGVDLFGFDQILPRDGRLDALAWSWAENEPSRAGDCTI
                     QRGDGRWIARRCTKRHRAACRRPDGRWVLTTSSLPAESALKACRARGGIPALPRTAAE
                     NEVLRSVAAAAGADGVWLPA"
     gene            2525..3706
                     /locus_tag="VF517_09885"
     CDS             2525..3706
                     /locus_tag="VF517_09885"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8053293.1"
                     /translation="MLRAIDRQGGTRPLACVMGDVDLVRALGMAGIRSACVTRPGWPA
                     DRSRFTAERIEWADNWGDPERLLDNVVRFAAAMPEPPPLFFQHDGDLMFVSRHRAALS
                     EHVRFAIADAELIEQCVDKTLFVVLAQRIGLPIPRSAVLWPAVQTGLPQLDLEPPLIL
                     KPLTRRDDWWQPLAGGAKAIEVDSLDELEREWSRFAEAGVDLVVQELIPGGEERIESY
                     HCYVDPNGDPAAEFTGRKLRTYPRRFGGTTACVITAEHDVLEMGRDCVRRMGLTGVAK
                     LDFKRDPSGGLHLLEVNPRFNLWHLPGAAAGVNIPAVVYADLVGLPRPSVGPVRPGVR
                     WSVPWHDYRAAREDGTSLVRWAAWQARCETHHNISLDDPMPFVGGMVLKRLRSRAGSG
                     G"
     gene            3706..>4398
                     /locus_tag="VF517_09890"
     CDS             3706..>4398
                     /locus_tag="VF517_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8053294.1"
                     /translation="MRYGVAGDVHGNVHALRTVLEALRAAGAERIVCPGDVVGYGPRP
                     DECVEMLADAGALVVAGNHDLMAIGRLPIERTGGIVRTTIEWTRSAISEATRSWLADL
                     PLELRTPDGLLVTHGGLGDPERYVRDRPAAAAELENLRERDPSARALLLGHTHRPMAA
                     PRGAPAVAREGGLALDPARGPWLLNAGSVGQSREARPVARALVFDSEGGAESARFLEL
                     SYDVRATRRELRA"
BASE COUNT          593 a         1644 c         1591 g          570 t
ORIGIN      
        1 cgcgtcggcg cgctcgcgcg cgatggccgt catccccgcc gccacgtccg acatcaccac
       61 ctcgccgccg ggagccacgc gctccgccgc ggccatgccc agcccgcccg tcccgcacgc
      121 gagctcgagc acctgctcgc ccggccgcgg ctcggtcagg tcgagcatgc gacccgtcat
      181 cccggacgcg agctcgtcga tgtactccgc gtgctcgccc caggccggcg cgaccgcgtt
      241 ccacatctga cgcaggcccg cccgcagatc gtcgccgccg ggcccgtccc cctgcgtcgc
      301 ggtgctctcg gtgttcaccc ctgccccctt cgtccgctgc cttggctaca gtgcccgtat
      361 attcattaca gtaacacgga actgaagtgg cgggcccggt caagcagcgt ccctacgact
      421 cgccgcggcg ccgcgcgcag gcggccgaca cccgccgcgc gatgctgctc gccgccgagc
      481 ggctcttcga gcgcgacggg ttcgcgcgca cgacgatcgc agccatcgcg gccgaggccg
      541 gcgtggcgga gaagaccgtc tacctcggct tcgagtccaa gagcgggctg ctgcgcgcgc
      601 tgtggaacct ccggctgcgt ggcgacgagg cggaggccgc cgtcgcgcag cgcgagtggt
      661 accgcgagat gctcgacgag cccgatcccg cacgcaagct gcgcctgacc gcgcgcaacg
      721 cacgcgtcgt gaaggagcgg atcggcccgc tcttcggagt catccgggac ggggcgccga
      781 cggacaccga cgtcgccgcg ctctgggagc ggatcgagag cgacttctac gacaaccagc
      841 tcgcgatcgt taagaccctc gccgcgatgg acgcgctgca tcccggcctg gacgccgctc
      901 gcggcgccga catcctctgg acgctgaacc acccggacgt ctggcgggcg ctcgtgagcc
      961 ggcgcggctg gagcgccgac cagtgggagg agtggttcgc gcagaccgcg gccgcgcagc
     1021 ttctcgccac aaagccggtt ggcaggaggc gccgcgggga atagggatgg gccagtggga
     1081 cgactcgccg cggttctcgc agcaggactc atcgccctag cgggggcgac cgccggcgcg
     1141 cacgcccaga cgctcgagga cacctgcgcc agcgccgagc tggtccccgg cgcgtgcgtc
     1201 ggcatctcca agatcaccga gcgcgccgcc gccgagtgcc gccgcgcggg agtcccggac
     1261 gagagctgca tcgcaccgat ggcccggcgc gtgatgcgcg aggaggtcag ggcgcacgcg
     1321 tcctcctggc tgcaccgcac gctcgacttt cagtacgagc tcggcagcag ggtgccattc
     1381 cgcgacgcgc cgtggatcgg cacgcacaac tcgttcaaca gcacctcgga gttcccgacg
     1441 gtctcgcaca ccgactcgaa ccagcagctc tcgctcacgc accagctccg gatcgacgtg
     1501 cgcagcctcg agctcgacgt gcactggctg ccgagcccgc acgcggccgg ccagccggcg
     1561 ccggtcgtct gccacggccg cggcgcggac gagctgcacg ccggctgcac caccgagcgc
     1621 ctgctgtccg acacgctcac cgagatcacc acctggctgc gcgaccacgg cgaccaggtg
     1681 atcctgctct acatcgagga tgccgtcgac gcgccggagg gctacgaccc gaccacggcg
     1741 gtgctcgacc aggggctgcg cgacgagcgt gggcggtcgc tggtctacag gcccccggcg
     1801 ggaggcgcct gcaccgacct gccgctcgat ctcacgcgcg agaaggtgcg cgcggccggc
     1861 gcccaggtcg tgatcgtgag cggctgcggg tcaggggccg cgtggcacgg gctcgtgttc
     1921 gattggaacg acgtccacgt cgagggcggc aacgacggct accgcgacgc gccggtctgc
     1981 gaccaggacc cggacggcga cggccggccc cagttcaatc gggagaccta cgccacgaag
     2041 ctggtgcggt tcttcgagga cagcacctgg ctgagcgcca cgctgaccgg cgagggggag
     2101 ggcctcaccc ccgagatcgc gcggcggatg tcgcgctgcg gcgtggatct gttcggcttc
     2161 gaccagatcc tcccgcgcga cggccggctc gacgcactcg cgtggagctg ggcggagaac
     2221 gagccctcgc gcgccggcga ctgcaccatc cagcgcggcg acggacgctg gatcgcccgc
     2281 cgctgcacga agcgacaccg cgccgcctgc cgccgccccg acggacgctg ggtgctgacc
     2341 acctcgtcgc tgcccgccga gtcggcgctg aaggcctgcc gcgcgcgtgg cggcattcca
     2401 gcgctcccgc gcaccgcggc cgagaacgag gtgctgcgca gcgtggccgc cgcggccggg
     2461 gccgacggag tgtggctacc ggcgtgagat gacggcccgc ggggcatcct gtctgcagag
     2521 gggcgtgctg agggctatcg acagacaggg gggcacgcga ccgctcgcgt gcgtgatggg
     2581 cgacgtggac ctggtccggg cgctcgggat ggccggcatc cgctcggcgt gcgtcacgcg
     2641 gcccggatgg ccagccgacc gctcgcgctt caccgcggag cgcatcgagt gggccgacaa
     2701 ctggggtgac cccgagcggc tgctcgacaa cgtcgtccgg ttcgccgccg cgatgcctga
     2761 gccgccaccg cttttcttcc agcacgacgg tgacctgatg ttcgtgtcgc gccaccgcgc
     2821 cgcgctcagc gagcacgtgc gcttcgcgat cgccgacgcg gagctgatcg agcagtgcgt
     2881 ggacaagacg ctcttcgtgg tgctcgcgca gaggatcggg ttgccgatcc cgcgctccgc
     2941 cgtgctctgg ccggccgtcc agaccgggct accgcagctc gacctcgagc cgccgctcat
     3001 cctcaagccg ctcacgcgcc gcgacgactg gtggcagccg ctcgccggcg gagcgaaggc
     3061 gatcgaggtg gactcgctgg acgagctcga gcgcgagtgg tcgcgcttcg ccgaggcggg
     3121 ggtggatctc gtcgtgcagg agctgatccc gggcggcgag gagcgaatcg agagctacca
     3181 ctgctacgtc gatccgaacg gcgacccggc ggccgagttc accggccgca aactgcgcac
     3241 gtatccgcgg cgcttcggag ggacgacggc ctgcgtcatc accgccgagc acgacgtgct
     3301 ggagatgggc cgcgactgcg tgcggcggat ggggctgacc ggggtggcca agctcgactt
     3361 caagcgcgac ccgagcggcg gcctgcacct gctcgaggtc aacccgcgct tcaacctgtg
     3421 gcatctgccg ggcgccgccg cgggcgtgaa catcccagcg gtcgtgtacg cggacctggt
     3481 cggcctgccg cgcccgagcg tcggtcccgt ccgccccggc gtgcgctggt ctgtgccgtg
     3541 gcacgactac cgcgccgctc gcgaggacgg cacgtcgctg gtccgctggg ccgcgtggca
     3601 ggcgcgctgc gagacccacc acaacatctc gctcgacgac ccgatgccgt tcgtcggcgg
     3661 gatggtcctg aagcggctgc gctcgagggc cggctccggc ggctgatgcg ctacggcgtt
     3721 gcgggcgacg tccacgggaa cgtccacgcg ctgagaacgg tcctcgaggc gctgcgtgcc
     3781 gcgggggcgg agcgcatcgt ctgccccggc gacgtggtcg gctacgggcc gcgccccgac
     3841 gagtgcgtgg agatgctcgc cgacgccggg gcccttgtcg tcgccggcaa ccacgacctg
     3901 atggcgatcg ggcgcctgcc gatcgagcgc acgggtggga tcgtgcgcac cacgatcgag
     3961 tggacccgct cggcgatctc ggaggccacc cgcagctggc tggccgacct gccgctcgag
     4021 ctgcgcaccc ccgacggcct cctcgtgacc cacggcgggc tcggcgaccc ggagcgctac
     4081 gttcgcgacc gccccgcggc ggcggcggag ctcgagaacc tgcgcgagcg cgacccgagc
     4141 gcccgcgccc tgctgctggg gcacacccac cggcccatgg cggcgccgcg cggtgcgccc
     4201 gcggtcgccc gcgagggcgg cctggccctc gaccccgcgc gcggcccgtg gctgctgaac
     4261 gccggcagcg tcggccagtc gcgcgaggcg cggccggttg cgcgcgcgct cgtgttcgac
     4321 agcgagggcg gcgccgagtc ggctcgcttc ctcgagctct cctacgacgt gcgcgcgacc
     4381 cgccgcgagc tgcgcgcg
//
LOCUS       DASVPL010000190        12679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_22100, whole genome shotgun sequence.
ACCESSION   DASVPL010000190 DASVPL010000000
VERSION     DASVPL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12679
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22100"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VF517_09895"
     CDS             complement(<1..589)
                     /locus_tag="VF517_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053295.1"
                     /translation="MARRSLKPQLNQIRSWVQQGRTDAWIAHQLDVSVGELVGFKREH
                     ELAPPEVDPDDGGAPGSAGEEEQDLRAQDEALIAAALDAEPDEEPEDTADGDGDAEGE
                     DRPRRRRGRRGGRSRRGRRRPLEGTFDHGDEGYGLWLDPAVQDDPIYAEHWAGHRPVT
                     ISVERDQIVIRRAGEEAGEGDDGAGEGGDDGGGGTG"
     gene            766..1008
                     /gene="csrA"
                     /locus_tag="VF517_09900"
     CDS             766..1008
                     /gene="csrA"
                     /locus_tag="VF517_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX8053296.1"
                     /translation="MLVLTRKSNQSIMIGDDIEVSVLAIMGEKVRIGIEAPRSVPVFR
                     KEVYLEIQQDSSRPEEDRKAVDEALDQMKSKGPGAA"
     gene            complement(1027..1269)
                     /locus_tag="VF517_09905"
     CDS             complement(1027..1269)
                     /locus_tag="VF517_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053297.1"
                     /translation="MRMRQSLAQFEAAFHEETAEERAMRERLRREAAVRSRQRRQARV
                     QRGGTFRFLVLLLSILATSVLVTVVMFRMLALWFGP"
     gene            complement(1335..2261)
                     /locus_tag="VF517_09910"
     CDS             complement(1335..2261)
                     /locus_tag="VF517_09910"
                     /inference="COORDINATES: protein motif:HMM:NF021389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEX8053298.1"
                     /translation="MALGYDGKLFILAFDHRGSFQKKWFGIEGEPDADETQTIADAKH
                     LIFEGLLSAAEGDIDPAVTGALVDAQFGGPVPTQAKSRGFKLAMPVEKSGQNEFDFQY
                     GDDFGKAIESADPDFSKVLVRYNSDGDAEMNARQLERLKRLSDWLHENDRKFLFELLV
                     PAEDAQLEQVGGDTDRYDAELRPELMRRAIEDIQDYGIEVDIWKIEGVDERSDCEMLA
                     QTTRRDGRDGVVCVLLGRGANDEKVDHWLTQAAPVDGFVGFAIGRSIWGDALKQFLDG
                     SLDRETAAKQIGDNYTRFVRVYQDAESAVGAS"
     gene            2339..3445
                     /locus_tag="VF517_09915"
     CDS             2339..3445
                     /locus_tag="VF517_09915"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX8053299.1"
                     /translation="MEHRFTGPSYTLGIEEELMIVDSETFELQNSIETMLEAMPQAEG
                     QVKPELMESVCEIATTPCKNTREAGAQLRDLRRLTQRTAGENGLAIGSAGTHPFAMWE
                     DQRIVARPRYRDLVAALRFVARQELIFGVHVHVGVDDPDKAIHIANGMRVHVPLLLAL
                     SANSPFWRADPSGLLSTRTPIFRAFPRVGIPPSYADFDDYSKRVGFMIESGAIEDYTY
                     LWYDVRPHPNFGTVEIRVMDGQTRLEHTLALAALVQALVKELAEHFESGQELSEYPYE
                     MLDENKWLAARHGLDGELVDLPSKDRVSTKELVRRVRDRIRGHSEELGSVDDLAGIDD
                     LLQRGNGATRQIVVYEANHDLREVMGEIVGATAA"
     gene            3466..4224
                     /locus_tag="VF517_09920"
     CDS             3466..4224
                     /locus_tag="VF517_09920"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8053300.1"
                     /translation="MSEADLFVVCKSCGSEVSPYVTECPYCGNRVRKRAPKIDRSEDD
                     ATQPRRRRRPKQPKLPKLRSDEIPGIAPETRPYATIGLIALSLLATLVLASGEVSLID
                     LGGIYGPLDGDWWRIAAYPFVFDNFGTVFITLVAVGIFGSLLERRFGPVAVVAIFLLA
                     GAAGGALTELLEAYPARSANGAAAGLLAAWYVDDRLAARRGDERGNDLLGVFVIFAVL
                     MLVPLADETASWAAGTGGLAAGAVLGALISPLRR"
     gene            complement(4329..5333)
                     /gene="selD"
                     /locus_tag="VF517_09925"
     CDS             complement(4329..5333)
                     /gene="selD"
                     /locus_tag="VF517_09925"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX8053301.1"
                     /translation="MLRLTELSHGAGCACKLDPGVLHPLLAQLPRSADPALLVGSEDA
                     DDAAVYRLTDELALVSTTDFFTPIVDDPYDFGRIAATNALSDVYAMGGTPLVALNLVA
                     YSLEQLGSEPLREILRGGGDVAAAAGVAIAGGHSIDDSEPKYGLAVTGTVHPDRVLRN
                     STARAGDALFLTKPVGGGAITTALKRGKGEAFLAGAVSVMTELNRDASLAAVDCGASA
                     VTDVTGFGLLGHLHEMCLGSGVEAEVAAGAVPAIEGVVELLAGDEPPVAGGTRRNRAW
                     VEPHVMWSDGVPEHLRWLACDAMTSGGLLVAAREDSGAPGVQIGRLAAGEPGRITVSA
                     "
     gene            5352..5681
                     /locus_tag="VF517_09930"
     CDS             5352..5681
                     /locus_tag="VF517_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053302.1"
                     /translation="MPERPLDDAAIDAALEALTDSKRLSAAESRVARVAPQLQRILNQ
                     ALAEGGWFGEAHEAQLLKAATEPDQEARLRLVRTLVAEETRMGMMIGVAVGWELAREL
                     DPHQQED"
     gene            5688..6365
                     /locus_tag="VF517_09935"
     CDS             5688..6365
                     /locus_tag="VF517_09935"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX8053303.1"
                     /translation="MDIRFLGHACFELTDGDTRVLIDPFLTGNPKAAAKADELDPTHI
                     FLTHGHADHIGDTVDIAKRSGAQVVAIVELANELSESGVENVADPNIGGTVEFDGVSV
                     RLTPAWHTAVSPSGTAHMPAGLVIELGGKTVYHLGDTGLFSDLALPARRSDIDVALMC
                     IGGHYTMDRHDGVIAAELVAAKQVIPCHYDTFPPIETDAQAFKSEVESRTSAEVVVLD
                     PGATHST"
     gene            6412..7326
                     /locus_tag="VF517_09940"
     CDS             6412..7326
                     /locus_tag="VF517_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX8053304.1"
                     /translation="MSETASDRMEREDFQATGDPAVQSASDSGHANLLSYEELYRLWE
                     RQHWSAHAIDFTQDRHDWWERIPEQERFQRMYGLSSFFIGEQKVADELGPMMRAAPTE
                     QMRIFMCTQIADEARHVELFNRFYDEVGVLDSPDLHGRLEAAQEHVNPEFNVIFDEML
                     GGRVDRLARKPEDLELLVETITLYHMVIEGMLALTGQHFILEYNERVGTLPGFIEGFN
                     NIARDEHRHVAFGARFLRDMAAEDPRYVDAIQRVLVEVAPIAESVLRPKWADEQEDLF
                     GVSPEETRNFAAKALERRLKVIGLTPVG"
     gene            complement(7377..8876)
                     /locus_tag="VF517_09945"
     CDS             complement(7377..8876)
                     /locus_tag="VF517_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8053305.1"
                     /translation="MSKRSGNGSLETLEAGALQHSSQLPVLAGRSPLLRLQSDEKLIA
                     MTRRGQHGAFETLVQRYQSRLLAFCRHMLGSTEDAEDVLQEVFTAAFNAMLADERPIN
                     ARPWLYRIARNRCLNHLRRPSASGQDSMDVFERDGGTTTADTVHKREEFRQIVADVQE
                     LPETQKTALLLREIDALSYEQIAEAMDTTVPSVKSLLVRARVSLGEAAEARLLSCSEV
                     RVELGEVAEGLARTSAPVRRHLKGCERCRTFRAELRRTNRALAAVFPIGPLVALKLFV
                     AKLGVGAAASGGSTAGSGIAASAGGAGATAGGATAGGALSAGVAGLASKAAAGIAAAA
                     LVTAGAVEVREVATHVNDERGKPAAVAVVAPRPAAPVQARASAAPRPVAVAVAAGPGP
                     SAKEPIEAEPAVPATAAATTAPVADQTSTTTPPATTAPAADTTAPTQPEDQGSTQEDG
                     STVVLTPGDDETAGGGPGSSSGSGGPATPPGERAPTGPASSGSVPTAGE"
     gene            8937..9021
                     /locus_tag="VF517_09950"
     tRNA            8937..9021
                     /locus_tag="VF517_09950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8971..8973,aa:Leu,seq:taa)
     gene            complement(9033..10283)
                     /locus_tag="VF517_09955"
     CDS             complement(9033..10283)
                     /locus_tag="VF517_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8053306.1"
                     /translation="MNAQANTPDHPGQLEKRSWIEVAKRTVKEFREDNLTDWAAALTY
                     YAVLALFPALIVIVSVLGLIGESATQPLIDNLGKVAPGPAKDIFTNAVTNLQENQGTA
                     GFAFVTGLAAALWSASGYTAAFMRASNAIYEVEEGRPIWKSLPVRVGVTLALVVGTLI
                     CAVAVVFTGGLAQAAGDVIGLGDSAVTAWSIAKWPVLLLIVSFMFALLYWAAPNVKQP
                     GFRWITAGGLFAVVVWILASTAFAFYVANFGSYNKTYGTLGGVISFLVWLWVSNIAVL
                     LGAEFNAELERGRQIEAGMKPTEREPFLPPRDTKKMDDPPDTSGIPDPTERGKKEDDD
                     VDDDKEDKGGRFARGEGSEGSEGSEGNGSDGEGFEPRKASDADLRREDRPNLAEGGGF
                     EPRTAAPKSGRVPGGGEPQLPDRD"
     gene            10335..10610
                     /locus_tag="VF517_09960"
     CDS             10335..10610
                     /locus_tag="VF517_09960"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HEX8053307.1"
                     /translation="MKFLFIPFSVISGLLAGFVGKKVFEQIWSVIDNEEPPESEHRDA
                     TWGKVIAAAALQGAIFRATKVAVDRSSRGAFESLTGTWPGQERPDPE"
     gene            10815..12218
                     /locus_tag="VF517_09965"
     CDS             10815..12218
                     /locus_tag="VF517_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053308.1"
                     /translation="MAAIGAVTIAIVVVAVLLFRGGGGHTLTAEFQNASQLVKGNQVT
                     IGGSPVGSVADIKLTDDGRADVKLSIKDDYKDIPVETQAVIRQASQSGIANRYVELQL
                     PPENKRSGKNLEDGGRIPITKTVTLVELDQLFNTLDPDTRDAVSKFFRNSADQFRGKE
                     EQQREAFRYLNPALSTSSRLFSELNRDSPLLADFLQDSEKVVTALAERRDDLTGLVTN
                     ANRTFQALSSEREALGESIARLPDFMRTSNTVFVNLRAALNDVDPLVNASKPVARQLR
                     PFLGELRPLAADARPTIRDLSNTLLQRGADNDLLDFQNTLPALASTALDKKERRPDFG
                     TGPKSVGNVPGAFPQTVDALNDAAPIIGYGRPFTPDLWGWFDDFSTSGPYDAEGGFSR
                     AQIVFNIQNVASAVPELIDLGQRDQVFGQLAKTGQFRRCPGGSEEPAPDGSNVFSASE
                     QQALDCVEADRATGPSP"
     gene            12218..>12679
                     /locus_tag="VF517_09970"
     CDS             12218..>12679
                     /locus_tag="VF517_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053309.1"
                     /translation="MRRIGTLLALVAVCAFAVIATGADDDGPKGKTIKIAFDNAFGLT
                     QGGDLRVAGVRAGQTSEFKTSRGPECQTEETKKTGRRPRTCALVIAKITEDGFKSFRV
                     DSQCDIRQQSLIGEYYVDCQPGNKPAKQPDGQPIPVQRTTSTVPTDLVNNIL"
BASE COUNT         1887 a         4552 c         4412 g         1828 t
ORIGIN      
        1 cgcccgtgcc tccgcccccg tcgtccccgc cttcgcccgc gccgtcgtca ccctcgccgg
       61 cctcctcgcc ggcgcggcgg atcacgatct ggtcgcgctc gacgctgatc gtcaccggcc
      121 ggtgcccggc ccagtgctcg gcgtagatcg ggtcgtcctg cacggcgggg tcgagccaga
      181 gcccgtagcc ctcgtcaccg tggtcgaagg ttccctcgag cgggcgccgg cggccgcgcc
      241 gcgagcgccc accgcggcgg ccacggcgcc ggcgcggccg gtcctcgccc tccgcgtcgc
      301 catcgccgtc ggccgtgtcc tccggctcct cgtcgggctc ggcgtcgagg gcggcggcga
      361 tcagcgcctc gtcctgcgcg cgcaggtcct gctcctcctc gcccgcgcta cccggcgcgc
      421 ccccgtcatc cgggtcgacc tcaggcggcg ccagctcgtg ctcgcgcttg aagcccacga
      481 gctcgcccac gctcacgtcg agctggtggg cgatccaggc gtccgtgcgg ccctgttgga
      541 cccagctccg gatctggttc agctgcggct tgagagagcg acgcgccatc gggcgcggaa
      601 ccctaccagc gggcttcaac ctcccggctc cccccgaacg ggtgactcgc gtccgcttgc
      661 gtgtacccgt acaatcttcc gatctcggga aaggattcct tgagtagcgg agaagctcca
      721 ctttacgagc gaccggcccg gcggctaggg ttgcccggag gcgccatgct ggtccttacg
      781 cggaagagca atcagagcat catgatcggg gacgacatcg aggtgtcggt cctcgcgatc
      841 atgggcgaga aggtccggat cgggatcgag gcgccgcgtt cggtgcccgt cttccgcaag
      901 gaggtctacc tcgagatcca gcaggactcg agccggcccg aggaggaccg caaggccgtc
      961 gacgaggcgc tcgaccagat gaagtcgaag ggcccgggcg ccgcttaggt ccgctctggg
     1021 cccggcctag ggcccgaacc agagcgccag catcctgaac atcacgacgg tcaccaggac
     1081 cgacgtggcc aggatcgaca gcagcagcac caggaagcgg aaggtgccgc cgcgctgcac
     1141 ccgcgcctga cggcgctggc gcgagcggac cgcggcctca cgccgcagcc gttccctcat
     1201 cgcccgctct tcggcggtct cctcgtggaa ggccgcctcg aactgggcaa gcgactgacg
     1261 cattcgcatt gggctagagc ctatcccgcc gcgcgtcccc atgaacgggg actacgtggc
     1321 ggcgcggcgc ctctctacga ggcgccgacc gccgactcgg cgtcctggta cacacgcacg
     1381 aagcgcgtgt agttgtcgcc gatctgcttc gcggcggtct cgcggtcgag agagccgtcg
     1441 aggaattgct tgagcgcatc gccccagatg ctgcggccga tcgcgaagcc gacgaagccg
     1501 tccacggggg ccgcctgggt gagccagtgg tcgaccttct cgtcgttggc accgcggccg
     1561 agcagcacgc agaccacccc gtcgcggccg tcgcggcggg tcgtctgggc gagcatctcg
     1621 cagtcggagc gctcgtccac gccctcgatc ttccagatgt ccacctcgat gccgtagtcc
     1681 tggatgtcct cgatggcgcg gcgcatcagc tcggggcgca gctcggcgtc atagcggtcg
     1741 gtgtcgccgc cgacctgctc gagctgggcg tcctcggccg gcaccagcag ctcgaagagg
     1801 aacttgcggt cgttctcgtg gagccagtcg gagaggcgct tgaggcgctc gagctggcgg
     1861 gcgttcatct cggcgtcgcc gtccgagttg taacggacga gcaccttcga gaagtcgggg
     1921 tcggcgctct cgatggcctt gccgaagtcg tcgccgtact ggaagtcgaa ctcgttctgc
     1981 ccgctcttct cgaccggcat cgcgagcttg aagccccggc tcttggcctg cgtgggcacg
     2041 gggccgccga actgcgcgtc cacgagcgcc cccgtcaccg ccgggtcgat gtcgccctcg
     2101 gcggcgctca gcagcccctc gaagatcagg tgcttggcgt cggcgatggt ctgcgtctcg
     2161 tcggcgtccg gctcaccctc gattccgaac cacttcttct ggaacgagcc ccggtggtcg
     2221 aacgcgagga tgaagagctt gccgtcgtag ccgagtgcca tgtgaagtcg ctacctgcct
     2281 tggttgttga cggcccgcag gatacccccg accggcacgc cacctaccat gccgccggat
     2341 ggagcaccgg ttcaccggcc cgagctacac gctcgggatc gaggaggagc tcatgatcgt
     2401 cgactccgag acgttcgagc tccagaactc gatcgagacg atgctcgagg cgatgcccca
     2461 ggccgagggc caggtgaagc ccgagctgat ggagtcggtg tgcgagatcg cgaccacccc
     2521 gtgcaagaac acgcgcgagg ccggcgccca gctccgcgac ctgcggcggc tcacccagcg
     2581 gaccgccggt gagaacggcc tcgcgatcgg ctcggcgggc acgcacccgt tcgccatgtg
     2641 ggaggaccag cggatcgtcg cccgcccgcg ctaccgcgac ctcgtcgcgg cgctcaggtt
     2701 cgtggcacgg caggagctga tcttcggggt ccacgtgcac gtgggcgtcg acgacccgga
     2761 caaggcgatc cacatcgcca acggcatgcg cgtgcacgtg ccgctgctgc tcgcgctgtc
     2821 ggccaactcg ccgttctggc gggccgaccc gagcggcctg ctctccaccc gaacgccgat
     2881 cttccgcgcg ttcccgcgag tcgggatccc gcccagctac gccgacttcg acgactactc
     2941 gaagcgtgtc ggcttcatga tcgagagcgg cgccatcgag gactacacct acctctggta
     3001 cgacgtgcgc ccgcatccga acttcggcac cgtcgagatc cgggtgatgg acgggcagac
     3061 gcggctcgag cacacgctcg ccctcgccgc gctcgtccag gctctcgtga aggagctggc
     3121 cgagcacttc gaatccgggc aggagctgtc cgagtacccc tacgagatgc tcgacgagaa
     3181 caagtggctc gccgcacgcc acggcctcga cggcgagttg gtggacctgc cgagcaagga
     3241 ccgcgtgtcg accaaggagc tcgtccggcg cgtgcgcgac cgcatccggg gccactccga
     3301 ggagctgggc tccgtcgacg acctggccgg catcgacgac ctgctccagc gcgggaacgg
     3361 cgccacccgg cagatcgtgg tgtacgaggc caaccacgac ttgcgcgagg tcatgggcga
     3421 gatagtgggg gcgaccgcgg cgtagcccag gggtatcctc gacctgtgag cgaggcggac
     3481 ctcttcgtcg tctgcaagag ctgcgggtcg gaggtcagcc cgtacgtcac cgagtgcccg
     3541 tactgcggca accgggtccg caagcgcgcc ccgaagatcg accgcagcga ggacgacgca
     3601 acgcagccgc gccggcgccg tcggcccaag cagccgaagc tcccgaagct gcgctcggac
     3661 gagatcccgg gcatcgcgcc ggagacgcgc ccgtacgcga cgatcgggct catagcgctg
     3721 agcctgctcg ccacgctcgt cctggcgagc ggcgaggtgt cgctgatcga cctcggcggg
     3781 atctacggcc cgctcgacgg cgactggtgg cggatcgccg cgtacccgtt cgtcttcgac
     3841 aacttcggca cggtgttcat cacgctcgtg gcggtgggga tcttcggatc gctgctcgaa
     3901 cggcgcttcg gcccggtcgc cgtcgtggcg atcttcctcc tggccggcgc ggccggcggc
     3961 gcgctcacgg agctgctcga ggcctacccg gcgcggagcg cgaacggcgc cgccgccggc
     4021 ctgctggcgg cctggtacgt ggacgaccgc ctggcggccc gccgcggcga cgagcgcggc
     4081 aacgacctcc tcggcgtctt cgtgatcttc gcggtgctga tgctcgtgcc gttggccgac
     4141 gagacggcct cgtgggcggc gggcacgggc ggcctggcgg cgggcgcggt gctgggcgcg
     4201 ctgatctcgc cgctgcgccg ctaggtcaac ggcggcactt aggaggggcg ggatcggccg
     4261 cgcgcggtcc tcttctcggt ggggtttcct gccttggagg gacagtgtcc ccctaagtca
     4321 ggaaagcttc aggcggagac cgtgatccgg cccggctcgc ccgctgcaag ccggccgatc
     4381 tggacgccgg gagcgcccga gtcctcgcgc gctgccacca ggagcccgcc cgaggtcatc
     4441 gcgtcgcagg cgagccagcg caggtgctcc ggcacgccgt cggaccacat gacgtgcggc
     4501 tcgacccaag cgcggttgcg gcgcgtgccc ccggcgaccg gcggctcgtc gccggccagc
     4561 agctcgacca cgccctcgat agccggcacc gcgccggccg cgacctcggc ctccacgccg
     4621 ctccccaggc acatctcgtg caggtggccg agcagcccga agccggtcac gtcggtcacc
     4681 gcggaggcgc cgcagtccac cgcggccaag gacgcgtcgc ggttgagctc ggtcatgacg
     4741 gagacggccc ccgcaagaaa cgcctccccc ttaccccgct tcagtgccgt tgtgatggcg
     4801 ccgccaccca ccggcttcgt gagaaacagc gcatcccccg cccgggccgt cgagttgcgc
     4861 agcacgcggt ccgggtgcac ggtcccggtg acggccaggc cgtacttcgg ctcgctgtcg
     4921 tcgatcgagt gtccgccggc gatcgccacg cccgccgccg cggccacgtc gccgccgccg
     4981 cgcaggatct cgcgcagcgg ctcagacccg agctgctcaa gcgagtaggc gacgaggttc
     5041 agcgcgacca gcggcgtgcc gcccatcgcg tagacgtccg agagcgcgtt ggtggcggcg
     5101 atgcgcccga agtcgtacgg gtcgtccacg atcggcgtga agaagtcggt ggtcgagacc
     5161 agggccagct cgtccgtcag ccggtaaacg gccgcgtcgt ccgcgtcctc gctgccgacc
     5221 agcagcgccg ggtcggcgct gcgcgggagc tgcgcgagca gcggatgcag cacgccgggg
     5281 tccagcttgc aagcgcagcc ggcgccgtgg gagagctcgg tgaggcgaag caccggccta
     5341 ttatcggtga cgtgccggag cgcccgctgg atgacgccgc aatcgacgcg gcgctcgagg
     5401 cgctcaccga ctccaagcgc ctcagcgcag ccgagtcgcg cgtggcccgc gtggccccgc
     5461 agctccagcg gatcctcaac caggcgctgg ccgagggcgg ctggttcgga gaggcgcacg
     5521 aggcacagct cctcaaggcc gccacggagc ccgaccagga ggcccggctg cggctggtgc
     5581 gcacgctggt cgccgaggag acccgcatgg ggatgatgat cggcgtagcg gtgggctggg
     5641 agctggcccg cgagctcgac ccacaccaac aggaggactg agaaccaatg gacatccgct
     5701 ttctaggcca cgcctgcttc gagctgaccg acggcgacac gcgcgtgctg atcgacccgt
     5761 tcctcaccgg caaccccaag gccgcggcca aggccgacga gctcgacccg acgcacatct
     5821 tcctcacgca cgggcacgcc gaccacatcg gggacacggt cgacatcgcc aagcgcagcg
     5881 gcgcacaggt ggtggcgatc gtcgagctgg ccaacgagct gagcgagtcg ggcgtcgaga
     5941 acgtggccga cccgaacatc ggcggcacgg tcgagttcga cggcgtcagc gtgcgcctca
     6001 cgcccgcctg gcacaccgcg gtctcgccga gcggcacggc gcacatgccc gccggcctcg
     6061 tgatcgagct cgggggcaag accgtctacc acctcggcga caccgggctg ttctccgacc
     6121 tcgccctgcc cgcccgccgc agtgacatcg acgtggcgct catgtgcatc ggcggccact
     6181 acacgatgga tcgccacgat ggggtgatcg ccgccgagct ggtcgccgcc aagcaggtga
     6241 tcccctgcca ctacgacacc ttcccgccga tcgagaccga cgcgcaggcc ttcaagtcgg
     6301 aggtcgagtc gcggacgtcg gccgaggtgg tcgtgctcga tccgggcgcc acgcacagca
     6361 cctagcggtt agactggctg gccagccagt cgagccaagg ggaggacgag agtgagcgag
     6421 accgccagcg accgcatgga gcgtgaggac ttccaggcga caggcgaccc ggcggtccag
     6481 tccgcgtcgg acagcggcca cgccaacctg ctgagctacg aggagctcta ccggctctgg
     6541 gagcgtcagc actggagcgc gcacgcgatc gacttcacgc aggaccgcca cgactggtgg
     6601 gagcgcatcc ccgagcagga gcgcttccag cgcatgtacg ggctgtcgtc gttcttcatc
     6661 ggtgagcaga aggtcgccga cgagctcggc ccgatgatgc gcgcggcgcc gacggagcag
     6721 atgcggatct ttatgtgcac gcagatcgcc gacgaggcgc ggcacgtgga gctgttcaac
     6781 cgcttctacg acgaggtcgg ggtgctcgac tcacccgacc tgcacggccg gctcgaggcc
     6841 gcgcaggagc acgtcaaccc cgagttcaac gtgatcttcg acgagatgct cggcgggcgc
     6901 gtcgatcggc tcgcgcgcaa gccggaggac ctcgagctgc tggtggagac gatcacgctc
     6961 taccacatgg tcatcgaggg gatgctcgcc ctcaccggcc agcacttcat cctcgagtac
     7021 aacgagcgcg tgggcacgct gcccggcttc atcgagggct tcaacaacat cgcgcgcgac
     7081 gagcaccgac acgtggcctt cggggcgcgc ttcctgcgcg acatggccgc cgaggacccg
     7141 cgttacgtgg acgccatcca gcgggtgctc gtggaggtcg cgccgatcgc cgagtccgtg
     7201 ctgcggccga agtgggccga cgagcaggag gacctcttcg gggtctcgcc cgaggagacg
     7261 cggaacttcg cggcgaaggc gctcgagcgg cgcctcaagg tgatcgggct cacgccggtc
     7321 ggctgagcgg agagggccgc ggccgcagcc gcgggcggcg ccgaagcgcc ggggtgctac
     7381 tcgcccgcgg tcgggaccga gccggacgag gccggtccgg tcggtgcgcg ttcgccgggg
     7441 ggcgtggccg ggccgccgga tccgctcgac gacccggggc cgccgccggc ggtctcgtcg
     7501 tcgccggggg tgaggaccac ggtcgagccg tcctcctgcg tggagccctg gtcctcgggc
     7561 tgcgtggggg ccgtggtgtc ggccgccgga gcggtggttg ccggaggcgt ggtggtggag
     7621 gtctggtccg cgacgggcgc cgtggtcgcg gcggccgtgg cgggcaccgc cggctcggcc
     7681 tcgatcggct ccttcgcgct cggacccggc ccggccgcga cggctacggc caccggccgc
     7741 ggagccgcgg aggcgcgcgc ctgcaccgga gcggcgggtc gcggtgctac gacggcgacg
     7801 gcggcgggct tgccgcgctc gtcgttcacg tgggtggcca cctcgcggac ctcgacggcg
     7861 ccggccgtca cgagcgcggc ggcggcgatg cccgcggccg ccttcgaggc cagcccggcc
     7921 acgcccgcgg agagcgcgcc gcccgcggtg gcgccgccgg cggtggcgcc cgcgccgccc
     7981 gccgaggcgg cgatgccgct gccggcggtc gagccgccgc tcgcggcggc gccgacgccg
     8041 agcttcgcga cgaacagctt gagcgccacg agcgggccga tcgggaagac ggcggcgagc
     8101 gcgcggttgg tccggcgcag ctcggcgcgg aaggtgcggc agcgctcgca gcccttgagg
     8161 tgacggcgca ccggcgcgga cgtgcgcgcc aggccctcgg ccacctcgcc gagctccacc
     8221 cgcacctcgg agcacgacag caggcgcgct tcggcggcct cgccgagcga cacgcgggcg
     8281 cgcacgagga gcgacttcac gctcggcacc gtcgtgtcca tcgcctcggc gatctgctcg
     8341 taggagagcg cgtcgatctc gcgcagcagc agcgcggtct tctgcgtctc gggcagctcc
     8401 tgcacgtcgg cgacgatctg ccggaactcc tcgcgcttgt gcacggtgtc ggcggtggtg
     8461 gtcccgccgt cgcgctcgaa gacgtccatc gagtcctggc ccgaggccga cgggcggcgc
     8521 aggtggttca ggcagcggtt gcgcgcgatg cggtagagcc acgggcgcgc gttgatcggg
     8581 cgctcgtcgg cgagcatcgc gttgaacgcc gcggtgaaga cctcctgcag tacgtcctcg
     8641 gcgtcctcgg tcgagccgag catgtggcgg cagaacgcga gcaggcgcga ctggtagcgc
     8701 tggaccaggg tctcgaaggc gccgtgctga cccctgcggg tcatcgcgat cagcttctcg
     8761 tcgctctgga gcctgagcag gggagagcgc ccggcgagca ccgggagctg cgagctgtgc
     8821 tgtagagcgc ctgcttccaa ggtttcgagc gagccgttgc ccgaccgttt cgacacgcta
     8881 gcaccgggga agttgcgcat ctacagcggg attcgcactt aaactcgcgc ctcctggccc
     8941 gggtgatgga actggtagac agcggggcct taaaagccct tgcccctcag gggcgtgtgg
     9001 gttcgaatcc caccccgggc actgctcccg cgctagtcgc ggtcgggaag ctgcggctcg
     9061 ccgccgcccg gcacgcgacc ggacttcggc gccgccgtgc gcggctcgaa gccaccgccc
     9121 tcggcgaggt tcggccggtc ctcgcgccgt aggtcggcat cgctcgcctt gcgcggctcg
     9181 aagccctcgc cgtccgaccc gttgccctcg gagccctccg agccctccga cccctcgccg
     9241 cgcgcgaagc ggccgccctt gtcctctttg tcgtcgtcca cgtcgtcgtc ctccttcttc
     9301 ccgcgctctg tcgggtccgg gatgcccgag gtgtcgggcg ggtcgtccat cttcttcgtg
     9361 tcgcgcgggg gcaggaacgg ctcgcgctcg gtcggcttca tgcccgcctc gatctggcgg
     9421 ccgcgctcga gctcggcgtt gaactccgcc ccgagcagca cggcgatgtt cgagacccac
     9481 agccagacca ggaacgagat cacgccgccg agcgtgccgt aggtcttgtt gtaggagccg
     9541 aagttggcga cgtagaacgc gaacgcggtc gacgcgagga tccacaccac gaccgcgaac
     9601 agcccgcccg cggtgatcca gcggaagccc ggctgcttca cgttcggcgc ggcccagtaa
     9661 aggagcgcga acatgaagct cacgatcagg agcagcaccg gccacttcgc aatgctccac
     9721 gcggtcacgg cgctgtcgcc gaggccgatg acgtcgccgg ccgcctgcgc gagcccgccg
     9781 gtgaagacca cggccaccgc gcagatcagc gtgccgacga cgagcgccag cgtcacgccc
     9841 acgcgcacgg ggaggctctt ccagatcggg cgcccctcct cgacctcgta gatcgcgtta
     9901 gacgcgcgca tgaaggcggc ggtgtagccc gaggccgacc agagcgcggc cgcgagacca
     9961 gtgacgaacg cgaagccggc ggtgccctgg ttctcctgga ggttcgtgac cgcgttggtg
    10021 aagatgtcct tcgccgggcc cggcgcgacc ttgccgaggt tgtcgatcag cggctgcgtg
    10081 gccgactcgc cgatcaggcc gagcaccgag acaatcacga tcagcgccgg gaacagcgcg
    10141 aggacggcgt agtaagtgag cgccgcggcc cagtcggtga ggttgtcctc acggaactcc
    10201 ttgaccgtgc gcttggcgac ctcgatccag gagcgcttct cgagctggcc ggggtgatcg
    10261 ggcgtgttgg cttgtgcgtt catggtggct ttcggttacc cgccgacccc gcgcggtata
    10321 cgtttcctgg cgtcatgaaa ttcctattca tcccgttcag cgtcatcagc ggcctcctcg
    10381 cggggttcgt cggcaagaag gtcttcgagc agatctggag cgtcatcgac aacgaggagc
    10441 cgcccgagtc cgagcaccgc gacgccacat ggggaaaggt gatcgcggcc gccgcgctcc
    10501 agggcgcgat cttccgcgcg accaaggtgg cggtggaccg cagctcacgc ggcgcattcg
    10561 agagcctcac gggcacctgg ccggggcagg agcgtccaga cccggagtga gttggcgcta
    10621 agccgctaat tcagcgttag tcgcctcaac actgtcttaa cagccgttct tcgacaggca
    10681 atgaaagtgc ggcaaatctg cctcttcttc cggtagattt ccgcgccctg tagccccgga
    10741 gggtctggtg cggacccccc ggcttcggga ggagatcagg gcaagggatg cgatctggaa
    10801 gtcttgtggg acgagtggct gccatcgggg cggtcacgat cgcgatcgtg gtcgtggccg
    10861 ttctgctgtt ccgtggcggc ggcggccaca ccctcaccgc cgagttccag aacgcctcgc
    10921 agctcgtgaa gggcaaccag gtcacgatcg gtggctcgcc ggtcgggtcg gtcgccgaca
    10981 tcaagctgac ggacgacggg cgcgccgacg tgaagctctc gatcaaggac gactacaagg
    11041 acatcccggt cgagacccag gcggtcatcc gccaggcgtc gcagtcgggc atcgccaacc
    11101 gctacgtcga gctccagctc ccgccggaga acaagcgctc gggcaagaac ctcgaggacg
    11161 gcggccgcat cccgatcacc aagaccgtca cgctcgtcga gctcgaccag ctcttcaaca
    11221 cgctcgaccc cgacacgcgc gacgccgtct cgaagttctt ccgcaactcg gccgaccagt
    11281 tccgcggcaa ggaggagcag cagcgcgagg ccttccgcta cctcaacccg gcgctgtcca
    11341 cctcgagccg gctcttcagc gagctcaacc gcgactcccc cctgctcgcc gacttcctcc
    11401 aggacagcga gaaggtcgtg accgcgctgg ccgagcgccg cgacgatctc accgggctcg
    11461 tcaccaacgc caaccgcacc ttccaggcgc tgagctccga gcgcgaggcg ctcggtgagt
    11521 cgatcgcccg cctgcccgac ttcatgcgca cctcgaacac cgtgttcgtg aacctgcgcg
    11581 cggcgctcaa cgacgtcgac ccgctggtga acgcctcgaa gccggtcgcg cgccagctcc
    11641 gtccgttcct cggcgagctg cgcccgctcg ccgccgacgc gcggccgacg atccgcgacc
    11701 tcagcaacac gctgctccag cggggcgccg acaacgacct cctggacttc cagaacacgc
    11761 tgccggcgct cgcatcgacc gcgctcgaca agaaggagcg ccgccccgac ttcggcaccg
    11821 gcccgaagag cgtgggcaat gtccccggtg cgttcccgca gacggtggac gcgctgaacg
    11881 acgccgcgcc gatcatcggc tacggccgcc ccttcacgcc cgacctgtgg ggttggttcg
    11941 acgacttctc gaccagcggc ccctacgacg ccgagggtgg cttctcgcgc gcgcagatcg
    12001 tcttcaacat ccagaacgtc gccagcgccg tgcccgagct gatcgacctc ggccagcgcg
    12061 accaggtctt cgggcagctc gcgaagaccg gccagttccg gcgttgcccg ggcggctccg
    12121 aggagcccgc ccccgacggg tcgaacgtct tctccgcctc ggagcagcag gcgctcgact
    12181 gtgtcgaggc cgaccgagcc acggggccga gcccgtgatg cggcggatcg gcaccctcct
    12241 cgcgctcgtc gccgtgtgcg cgttcgccgt gatcgccacg ggcgcggacg acgacggccc
    12301 gaagggcaag acgatcaaga tcgcgttcga caacgccttc gggctcaccc agggcggcga
    12361 cctgcgcgtg gcgggcgtgc gcgcgggcca gacgtccgag ttcaagacct cgcgcggtcc
    12421 cgagtgccag accgaggaga cgaagaagac cggccgtcgc ccgcgcacgt gcgcgctcgt
    12481 gatcgccaag atcaccgagg acggcttcaa gtcgttccgc gtcgactcgc agtgcgacat
    12541 ccgccagcag tcgctgatcg gcgagtacta cgtggactgc cagcccggta acaagcccgc
    12601 caagcagccc gacggccagc cgatcccggt ccagcgcacg acctccaccg tcccgacgga
    12661 cctcgtcaac aacatcctg
//
LOCUS       DASVPL010000191         6937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_22107, whole genome shotgun sequence.
ACCESSION   DASVPL010000191 DASVPL010000000
VERSION     DASVPL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22107"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1302
                     /locus_tag="VF517_09975"
     CDS             214..1302
                     /locus_tag="VF517_09975"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX8053310.1"
                     /translation="MERALKPVADRTSGAASAQPQRPAAEGHGRRLTAAFEALEAFPA
                     LAESRNRVLRLVREEHTAAGDVVAAIESDVSLVIAVLRIANRAPAPKRGRISSIPEAI
                     ELLTPSGVEALASRTAVFDFFERIPGWDAAPERFRLHAVATQRAADRIAREIDFPDKD
                     ELLVSALLHDIGKLVLVHAYPGYPAQVHREARTPDERLHQERRELGVDHALVGGVLAR
                     RWGLPARLAAAIERHHSDDAQGEAAVIRLGDMLAHYAQGHPIEPARMLKVARELDLDA
                     DRLRSIMYELPYGGGQERRQVDPCPLSPREVDVLKRLAEGKVYKQIALELNLSTSTVR
                     THLHNTYAKLGAVDRAQAVLLATERGWI"
     gene            complement(1299..2486)
                     /locus_tag="VF517_09980"
     CDS             complement(1299..2486)
                     /locus_tag="VF517_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8053311.1"
                     /translation="MTIGRRLPLQSAEPAVWFAYLRAAIVVAGLVAVAAFDVPHRGRL
                     ALAIALLALPWALWLVAAARRSSTLVLSPAIAAGDLLILGLVQVAEPASYAGVRFVGL
                     FLIATHAYFQGELRGLAVAAAGIAFLVPVTALVDTPMDGGMLAFSEGLFAVSALCCGL
                     FVARLRTAESVERLRSRELSRRAIEAENEVRRRLAEALHDGPVQELMGLDLMLASLDT
                     AAARGDSDGVRERVAEARKVTERNIVALRDELVGLGPYAFDELSFDAAIEQCRPVWQR
                     RHSIGIELAVDRLDLTNEVCGALFGIAQEAVANAGRHAGASRVVVTLRAVDGEVELRV
                     SDDGHGFTGPSPLSSTTAGHIGLASMRERAELIGGRLEIETGERGTKVLVRAPLARPV
                     ATA"
     gene            complement(2483..2860)
                     /locus_tag="VF517_09985"
     CDS             complement(2483..2860)
                     /locus_tag="VF517_09985"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX8053312.1"
                     /translation="MTRTRRSPLALALLWAPPLVLMGVIFALSAQPDLSSGLGTIDLV
                     LRKAAHMTEYGLLVFLLWRPLRTIAADRAALVAAIGVGVLYAASDEWHQTFIEGRHGT
                     PVDVGIDSIGMTVAALLIVRSLR"
     gene            2886..3287
                     /locus_tag="VF517_09990"
     CDS             2886..3287
                     /locus_tag="VF517_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8053313.1"
                     /translation="MLAITSKSPYAVRALSELARVGGAGPVPIGEIARRRDIPVQFLE
                     GMFATLRRAGILQSQRGVKGGYLFARPPREVTVLDVVEALEGSLDGDLASADPIWAEA
                     IEALRERLRATTIAAIAEREAREAGAQMYYI"
     gene            complement(3284..3496)
                     /locus_tag="VF517_09995"
     CDS             complement(3284..3496)
                     /locus_tag="VF517_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053314.1"
                     /translation="MRMNEPTAVIRRSPIIFRRRLWTSDHPVVIEERTDLIHRPSASL
                     ILRGDMDLSSVAVRALLSAGAGAAPP"
     gene            3604..4281
                     /locus_tag="VF517_10000"
     CDS             3604..4281
                     /locus_tag="VF517_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053315.1"
                     /translation="MASRKEEKERLRQEREERERQEEEKSKKKKRRARMLGAAALLVL
                     ALAIIAFVAFSGSDKKSPEEAQKGLQATTGPWEPLTAGLEDRENALGLPAPSDTIYHV
                     HAQLKVFVNGKPMTVPNNIGIDPAKGPVSLHTHDDKGIIHMEAVEPFPFTLGEFFTIW
                     GVRFTPTTLGAFQAGNGLVLETYLNGKKVPNGPGLQMKQSDRVVVMFGKPNSAPKDFK
                     LDAGASG"
     gene            4480..5436
                     /gene="cysK"
                     /locus_tag="VF517_10005"
     CDS             4480..5436
                     /gene="cysK"
                     /locus_tag="VF517_10005"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX8053316.1"
                     /translation="MSQTSAGQARAAIPANLADLVGRTPMVRLTRLAPECGAEIVGKL
                     EAYNPAGSVKDRIGVAMIEAAEAEGRIEPGKSTIVEATSGNTGIALAFVCAAKGYRLV
                     LFMPQGMSREREGLLRLYGADVRETESMGGMNEAIEAALQFAEDCPHCFRPDQFSNPA
                     NPEIHRRTTAEEIWEDTGGSVDVLVAGVGTGGTITGAGERLKELNPDLRVVAVEPKSS
                     PVLSGGLPGPHKIQGIGAGFVPDVLNLEVIDEVIGVDDEDALDTARRVCAREGILAGI
                     SGGAALWAAVAVGSRPEHQGARVVAVVPDSGERYVSTPFFAP"
     gene            5433..6155
                     /locus_tag="VF517_10010"
     CDS             5433..6155
                     /locus_tag="VF517_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX8053317.1"
                     /translation="MTLTELERERYARQLAGWGEESQARLREANAIVVGLGSLGSPVA
                     LYLAAAGVGRLGVVDPAVVELGDLSRQPLHFTPDLGVPKAQNAAVKLRALNPEIVVEP
                     YPARVDAGNALAIVAGADVVVDCTGGAEVNDACCAAGIPLVWGATTGHVLAVRPGESA
                     CLRCVYPSELPLRLRGPGALAGIVGSMQALEALKQLSGVGRPLLDTLLQFDGETMEQA
                     LLPVARRDECPSCAVPAPAQSA"
     gene            6155..6895
                     /gene="cysE"
                     /locus_tag="VF517_10015"
     CDS             6155..6895
                     /gene="cysE"
                     /locus_tag="VF517_10015"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX8053318.1"
                     /translation="MPRLGALTRVARELRRDVRAAQDRDPAARGLARVEILLTYGGVH
                     ALLAYRVAHVLHEARVPLVPHAIAYLARSITGVEIHPGARIGEGLFIDHGTGVVIGQT
                     AEVGRNVTLYQQVTLGGTGFAPGKRHPTVGDDVVVGSGAKLLGPINVGRGAKVGANSV
                     VIHDVPPNSTVVGNPGHPVRVDGRRPVGPDADWAHLPDPVADAIRALAARVTELEQQL
                     AEATGREHRPPAEVRPLRDVRGGDPAGG"
BASE COUNT         1025 a         2561 c         2495 g          856 t
ORIGIN      
        1 cggatcgaca gcgccattgc ccgcgctgtc cccgataagg gtggcgcccc caccagctcg
       61 ggttagggtc cacgccccaa tcccgctcgc gcaccggcct cggcgctcaa ctccggaact
      121 tgctctgccg acaaagagtc accggacgga ttacctaccc gcccgccagc cgcccccctt
      181 acgaactcgc aacgccacgg aaggcccacc caagtggaaa gagcactcaa gccggtcgca
      241 gaccggacca gcggagccgc gtccgcccag ccccagcgcc ccgccgctga gggtcacggc
      301 cgccggctga ccgctgcctt tgaggctctg gaggccttcc cggcgctcgc cgagtcgcgc
      361 aaccgcgtgc tgcgcctcgt ccgcgaggag cacaccgccg cgggcgacgt ggtcgccgcc
      421 atcgagtcgg acgtctcgct ggtcatcgcc gtcctcagga tcgccaaccg cgcgccggcc
      481 cccaagcgcg gccgcatctc gagcatcccc gaggccatcg agctgctcac gcccagcggc
      541 gtcgaggccc tcgccagccg caccgcggtc ttcgacttct tcgagcgcat ccccggctgg
      601 gacgccgcgc ccgagcgctt ccgcctccac gcggtcgcca cccagcgcgc cgccgaccgg
      661 atcgcccgcg agatcgactt cccggacaag gacgagctgc tcgtctccgc gctgctccac
      721 gacatcggca agctcgtgct ggtccacgcc tatccgggct accccgccca ggtgcatcgc
      781 gaggcccgca cgcccgacga gcgcctgcac caggagcgcc gcgagctcgg cgtcgaccac
      841 gcgctggtcg gcggcgtact cgcccgccgc tggggcctgc ccgcacggct cgcggccgcg
      901 atcgagcgcc accactccga cgatgcccag ggcgaggccg ccgtcatccg cctcggcgac
      961 atgctcgcgc actacgcgca gggccatccg atcgagccgg cccgcatgct gaaggtcgcc
     1021 cgcgagctgg acctcgacgc cgaccgcctg cgcagcatca tgtacgagct gccctacggc
     1081 ggcgggcagg agcgccgcca ggtcgatccg tgcccgctct cgccgcgcga ggtggacgtg
     1141 ctcaagcgcc tcgccgaggg caaggtctac aagcagatcg ccctcgagct gaacctctcg
     1201 acgagcacgg tgcgcacgca cctgcacaac acctacgcga agctcggagc ggtcgaccgc
     1261 gcgcaggccg tgctgctcgc gaccgagcgc ggctggatct aggccgtcgc gacgggccgc
     1321 gcgagcggcg cccgcaccag caccttcgta ccgcgctcgc cggtctcgat ctcgagccgg
     1381 ccgccgatca gctccgcgcg ctcgcgcatg ctcgcgagcc cgatgtgccc cgccgtcgtg
     1441 ctcgacaggg ggctcggccc cgtgaagccg tggccgtcgt cggagacccg cagctcgacc
     1501 tcgccgtcca cggcccgcag cgtgaccacg acccggctcg cccccgcgtg gcggcctgcg
     1561 ttcgccaccg cctcctgcgc gatgccgaac agcgcgccgc acacctcgtt ggtcaggtcg
     1621 agccggtcca cggccagctc gatgccgatg ctgtgccggc gctgccagac cgggcggcac
     1681 tgctcgatcg cggcgtcgaa cgacagctcg tcgaacgcgt aggggccgag gccgaccagc
     1741 tcgtcgcgca gggcgacgat gttgcgctcg gtgaccttgc gcgcctcggc gacccgctcg
     1801 cgcacgccgt cggagtcgcc ccgcgccgcg gccgtgtcga gcgaggcgag catgaggtcg
     1861 agccccatca gctcctgcac ggggccgtcg tgaagcgcct ccgcgaggcg ccggcgcacc
     1921 tcgttctcgg cctcgatcgc gcgccgcgag agctcgcgcg agcgcagccg ctcgaccgac
     1981 tcggccgtgc gcagccgcgc gacgaacagg ccgcagcaga gcgccgagac ggcgaacagc
     2041 ccctcggaga acgcgagcat gccaccgtcc atcggggtgt ccacgagcgc ggtgacaggg
     2101 acgaggaacg cgatgcccgc ggcggcgacg gcgagccccc gcagctcgcc ctggaagtac
     2161 gcgtgcgtgg cgatcaggaa cagcccgacg aagcggacgc cggcgtagga ggcgggctcg
     2221 gccacctgca cgagcccgag gatcagcagg tccccggcgg cgatcgccgg gctcaggacc
     2281 agcgtggagc tgcggcgggc cgccgcgacg agccacagcg cccacggcag ggcgaggagg
     2341 gctatcgcga gcgcgaggcg gccgcggtgc gggacgtcga aggccgccac ggccacgagc
     2401 ccggcgacga cgatcgcggc gcgcaggtag gcgaaccaga ccgctggctc cgccgactgg
     2461 agcgggaggc ggcggccgat cgtcaccgca ggctcctcac gatcagcagc gcggcgacgg
     2521 tcatgccgat cgagtcgatc cccacgtcga ccggcgtgcc gtggcggccc tcgatgaacg
     2581 tctggtgcca ctcgtcgctc gcggcgtaca gcaccccgac cccgattgcg gcgacgagcg
     2641 cggcgcggtc ggccgcgatc gtgcgcagcg ggcgccacag caggaacacg agcaggccgt
     2701 actcggtcat gtgcgcggcc ttgcgcagga cgaggtcgat ggtgccgaga ccggacgaga
     2761 gatccggctg cgccgagagg gcgaagatga cccccatcag gacgagcggc ggcgcccaca
     2821 ggagcgcgag cgcgagcggt gagcggcggg ttcgcgtcac ctacataacc tagactcgcc
     2881 agggcttgct cgccataacc tccaagtccc cgtacgcggt gcgcgcgctc agtgagctgg
     2941 ctcgcgtcgg cggcgcgggg ccggttccga tcggcgagat cgcccgccgc cgcgacatcc
     3001 cggtccagtt cctcgagggc atgttcgcga cgctccgccg cgccgggatc ctccagagcc
     3061 agcgcggcgt caagggcggc tacctgttcg cccgcccgcc gcgcgaggtg actgtgctcg
     3121 acgtggtcga ggcgctcgag ggctcgctcg acggcgacct ggcgagcgcc gacccgatct
     3181 gggccgaggc gatcgaggcg ctgcgcgagc gcctgcgcgc gacgacgatc gccgcgatcg
     3241 ccgagcgcga ggcccgcgag gccggcgcgc agatgtacta catctagggc ggcgcggcgc
     3301 cggcgccggc gctcaagagc gcacgcacgg caaccgatga caagtccatg tcgccacgca
     3361 aaatcaagct ggcgctcggc cggtggatga ggtcggtgcg ctcttcgatg accaccggat
     3421 ggtcggacgt ccacagcctg cgccggaaga tgatgggact gcgcctgatg acggcggtgg
     3481 gctcgttcat ccgcatggcg ttgggtcgcg tcgactagta accggcagct cagaatctga
     3541 gcggcggtcg gcggtccgtc tttaggcgcg ccagaggcac ccaccgctag actcgcgcgc
     3601 cgcatggcca gtcgcaagga agagaaggaa cgcctgcgcc aggagcgcga ggagcgcgag
     3661 cgccaggagg aggagaagtc caagaagaag aagcgccgcg cccgcatgct cggcgcggcc
     3721 gctctgctgg tcctcgccct cgcgatcatc gcgttcgtcg cgttctccgg cagcgacaag
     3781 aagtcgcccg aggaggcgca gaaggggctc caggcgacca ccggcccgtg ggagccgctg
     3841 acggcgggcc tcgaggatcg cgagaacgcc ctcggcctac cggcgccgag cgacacgatc
     3901 taccacgtcc acgcgcagct gaaggtcttc gtgaacggca agccgatgac ggtgccgaac
     3961 aacatcggta tcgatcccgc caagggcccc gtgtcgctgc acacgcacga cgacaagggc
     4021 atcatccaca tggaggcggt cgagccgttc ccgttcaccc tcggggagtt cttcacgata
     4081 tggggagtgc gcttcacgcc gaccacgctc ggcgccttcc aggccggcaa cggcctcgtg
     4141 ctggagacct acctgaacgg caagaaggtc cccaacggcc cgggcctcca gatgaagcag
     4201 agcgaccgcg tggtcgtgat gttcgggaag cccaactcgg cccccaagga cttcaagctg
     4261 gacgccggcg cctccggtta gccgtccgac cggcgggggg ctaatacata cgtccggagg
     4321 gggttccgct ttcgggcgac ctgccgtagg ttgataacca ggcaggggac accttcagtc
     4381 tccagggact cagaagccgg ccggaccagg ccctaactac cacggtccgg ccggcttcga
     4441 ccgttctggg gccaccgggg cgccggttac cctctgaaca tgagccagac gagcgccggg
     4501 caggcgcgag cggctatccc ggcgaacctc gccgacctgg tcggccgcac cccgatggtc
     4561 cgcctcacgc gcctcgcgcc cgagtgcggc gcggagatcg tcggcaagct cgaggcctac
     4621 aacccggccg gcagcgtgaa ggacaggatc ggcgtggcga tgatcgaggc cgccgaggcc
     4681 gagggccgca tcgagcccgg caagagcacg atcgtcgagg ccacgtcggg caacaccggg
     4741 atcgccctcg cgttcgtgtg cgcggcgaag ggctaccgcc tcgtgctgtt catgccccag
     4801 gggatgagcc gcgagcgcga ggggctgctg cgcctctacg gcgccgacgt gcgcgagacc
     4861 gagtcgatgg gcggcatgaa cgaggcgatc gaggccgcgc tccagttcgc cgaggactgc
     4921 ccgcactgct tccggcccga tcagttctcg aacccggcca acccggagat ccaccggcgc
     4981 acgacggcgg aggagatctg ggaggacacg ggcggctcgg tcgacgtgct ggtggccggg
     5041 gtcggcacgg gcggcacgat caccggcgcc ggcgagcgcc tgaaggagct gaacccggac
     5101 ctgcgggtgg tggcggtcga gccgaagtcc tcgccggtgc tgtctggcgg cctgccgggg
     5161 ccgcacaaga tccagggcat cggcgccggc ttcgtccccg acgtgctgaa cctcgaggtg
     5221 atcgacgagg tcatcggggt cgacgacgag gacgcgctcg acaccgcccg ccgcgtgtgc
     5281 gcgcgcgagg ggatcctcgc cgggatctcg ggtggggcgg cgctgtgggc ggcggtcgcg
     5341 gtcggcagcc ggcccgagca ccagggtgca cgtgtggtgg cggtcgttcc cgactcgggc
     5401 gagcgctacg tgagcacccc cttcttcgcg ccgtgacgct gaccgagctc gagcgcgagc
     5461 gctacgcgcg ccagctcgcc gggtgggggg aggagtcgca ggcgcgcctg cgcgaggcga
     5521 acgcgatcgt cgtgggcctc ggctcgctcg gctcgccggt cgcgctctac ctcgccgccg
     5581 cgggggtggg tcgtctcggc gtggtcgacc cggccgtggt cgagctcggc gacctctcgc
     5641 gccagccgct ccacttcacc cccgacctcg gcgtgccgaa ggcgcagaac gccgcggtca
     5701 agctgcgtgc cctgaacccg gagatcgtgg tcgagcccta ccccgcgcgg gtcgacgccg
     5761 gcaacgcgct cgcgatcgtg gcgggcgccg acgtggtggt ggactgcacg ggcggcgccg
     5821 aggtcaacga cgcgtgctgc gccgcgggga tcccgctggt gtggggagcc acgacggggc
     5881 acgtgctcgc ggtccgcccg ggcgagtcgg cctgcctgcg ctgcgtatac ccctccgagc
     5941 tgccgctgcg actccgcggg ccgggcgcgc tggcgggtat cgtcggatcg atgcaggcgc
     6001 tcgaagcgct aaagcagctc agcggcgtgg ggcggccgct gctcgacacg ctgctccagt
     6061 tcgacggcga gaccatggag caggccctgc tccccgtcgc caggcgtgac gaatgtccgt
     6121 cgtgcgccgt gcccgctccg gcacaatccg cctgatgcct cgactgggag cgctcacccg
     6181 ggtggcacgg gagctgcgcc gcgacgtgcg cgccgcgcag gatcgcgacc cggccgcgcg
     6241 cgggctcgcg cgagtggaga tcctcctcac ctacggcggc gtgcacgcgc tgctcgcgta
     6301 ccgcgtcgcc cacgtgctgc acgaagcgcg cgtgccgctg gtgccgcacg cgatcgccta
     6361 cctggcgcgt tcgatcaccg gcgtcgagat ccaccccggc gcccgcatcg gcgagggcct
     6421 cttcatcgac cacggcaccg gcgtggtgat cgggcagacc gccgaggtcg gccgcaacgt
     6481 gaccctctac cagcaggtca cgctaggcgg gaccggcttc gcgcctggca agcgccaccc
     6541 gacggtgggg gacgacgtgg tggtgggatc gggggcgaag ctgctcgggc cgatcaacgt
     6601 cggtcgcggc gcgaaggtgg gcgcgaactc ggtcgtgatc cacgacgtgc cgccgaactc
     6661 cacggtggtg ggaaacccgg gccatcccgt ccgcgtggac ggccgccgcc ccgtggggcc
     6721 cgacgccgac tgggcgcacc tgcccgaccc ggtcgcggac gcgatcaggg cgctcgccgc
     6781 gcgggtcacc gagctcgagc agcagctcgc cgaggcgacc gggcgcgagc accgaccgcc
     6841 cgccgaggtg cgcccgctgc gcgacgtgcg cggaggcgac ccggccggcg gctgagccac
     6901 cggccgcggc gccgcgcgcc gggccggcgg ctcgcac
//
LOCUS       DASVPL010000192         5304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_334660, whole genome shotgun sequence.
ACCESSION   DASVPL010000192 DASVPL010000000
VERSION     DASVPL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334660"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VF517_10020"
     CDS             <1..684
                     /locus_tag="VF517_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053319.1"
                     /translation="EAVQRRQMQYISALYEGDAGRMYTALGELLVAPDESRLALFRRD
                     FAPPPRDGAPIAEWMVEVMRLVRRHGLRLPPRVLSMYRALFAAETAAAAFGARASLRS
                     VGRDFFARLRRKQAAEAFRREDLEAFAISLVGLLRDAPEHLERTLRSRAEGRFVLGSD
                     SIDAPDSAAARRARARMTAGGMTSIALAGALALPDLPEAAGVSAAWPLGALLCALYAS
                     LLLGWRRLG"
     gene            complement(645..1682)
                     /locus_tag="VF517_10025"
     CDS             complement(645..1682)
                     /locus_tag="VF517_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053320.1"
                     /translation="MEAAALMLARKDRMVRSKSTAMERAFDRRGPERYADEGGPLPPA
                     ETILEIRRLFAEITAGNDHEDLVYMLNALVQNREALQQAVIVWVDLIEVLVQSAEGRY
                     GAVGRGDLKKDEVKAVVVYLLRTGKFRIPNVPDYLQPVVVDVVADISVDAVVAVANQR
                     GLWVEPPRTARPTWHELRRRFLRGIRRVVGFVLEPFGRLAAAIVMALRYPVRLSPALR
                     QALEAIEREGMIADHQSLLQRITDLKIWIGNNAGSVIAGVQLVDLVVDEAEAFGDLTG
                     PEKKDYARELVLAVLEDLGFDVRGRFTGMKLEMAIDMAIDSLVHTLHKRGVFTHHAPQ
                     PRRLQPRSRLA"
     gene            complement(1664..3157)
                     /locus_tag="VF517_10030"
     CDS             complement(1664..3157)
                     /locus_tag="VF517_10030"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX8053321.1"
                     /translation="MLRRIARYLATRPDVLDTDERTRLADLGEHVPPMGWPDVRKVLM
                     EGLGAHPSELFATVEPRPIASQAIAQVHRGSLLDGTPVRIKLVRPEAPDAVARAVGGA
                     DALAGLLGDRGNGERPRLAEEVAAWLDLHLDLTNELANLERLWELAADSTWEFVPRPY
                     PDLCAPTVLVSEDPGGVPMSDVILAAGPGPPGRVRVLREQEIDPVRLARVVLTVNLRQ
                     AFRYRLFGVDLNPRNLVALPGDVISFVDFSHCGQIDPTMSEDQLAYLTAVFENDMERA
                     LDPPIETTPFAGEGNIAAFRRELLDLARGRAVGGSGEGRSPAPGAPSPAGQLLADVMR
                     AAWLHHVRLPEGGLLLYRSIVTAQETARRLDPDAGIDEVSRRYLRVSHITAKARKVDP
                     ERIQSSALNVLTLLRDSPGQLQKILSDLADGSFSLNVWESETPAAERSRNRRSRFLVV
                     ALCAVSLAVLLTVPSPPEPLGVSLAWPLGAALAVLYAWLVLEWRRLR"
     gene            complement(3232..4854)
                     /locus_tag="VF517_10035"
     CDS             complement(3232..4854)
                     /locus_tag="VF517_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX8053322.1"
                     /translation="MRLRDRVLEVRRALELAWTVARFGTRMVAGRFLPLRGPGLPVQI
                     REALEDLGLTYLKLGQYLAMRFDILPPEVCRELGRLFDEVAPLPAEVARSIVEEELGA
                     PIDERFARFESEPLAAASVAQVHRAWTRDGDAVAVKVQRPGIERVFSADIRIMRRLTR
                     LADALHLLGRLSATEMLEQFANWTIREIDFEQEGRTAELVARNAERYEIEPSVHWDLT
                     TSRVLTLEFIDGHSLARIIRVIEQDGAEGAAREYPWLDVDLVLHHLTFATLRQVFVTG
                     LFHGDPHPGNVLVLEDNRIAFVDFGIFGELAGFDRDTLGAQIEQLAIGNIDESLRAYE
                     RQLASTAESDVRAFRTEAREVLTRWYQLSLRSDSPIAERHLGRYIGEMIDISRRYRLL
                     YDMSFLLYWRTLNALDSTALRMSPTYDLMEELRRFFEEIRPDLIERVMGVAMDRRGWA
                     RVRGIGQEAPDRVAALIGEAARGSLVGTVFAGERTSARRARHAETRWLGAAVAGVALI
                     VVAGSGAHTAGLLAVATGLLLTAATLRGLAPR"
     gene            complement(4845..>5304)
                     /locus_tag="VF517_10040"
     CDS             complement(4845..>5304)
                     /locus_tag="VF517_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEX8053323.1"
                     /translation="ARRLGDRAAPPVHLFVAAHVAPHLHGDVPATHLLPRDELVARLG
                     AMGGTPSAVLDNTALMDHFLPIVRADLGLCADYVYDPTAGRFLTIPISAFGATSDPEV
                     PVCAIGPWADLTRGPFRLRLIPGNHFFAARAPEQLMRAIEHDLASRVPCG"
BASE COUNT          737 a         1877 c         1997 g          693 t
ORIGIN      
        1 gaggccgtcc agcgacggca gatgcagtac atctcggccc tgtacgaggg cgacgccggg
       61 cggatgtaca cggcgctcgg tgagctgctg gtcgcccccg acgagagcag gctggcgctg
      121 ttccggcgcg acttcgcgcc gccgccgcgc gacggagcgc cgatcgccga gtggatggtc
      181 gaggtcatgc ggctggtgcg ccggcacggg ctgcggctgc cgccgcgcgt gctgtcgatg
      241 taccgcgcgc tgttcgccgc cgagacggcg gccgccgcct tcggcgcgcg cgcctcgctg
      301 aggtcggtgg ggcgcgactt cttcgcccgg ctgcgccgca agcaggccgc cgaggcgttc
      361 cggcgcgagg acctcgaggc gttcgcgata agcctggtgg gactgctgcg cgacgcgccg
      421 gagcacctcg agcgcacgct gcgaagccgt gccgagggcc gcttcgtgct cggctcggac
      481 tcgatcgacg cgccggacag cgcggccgcc cgccgcgccc gggcgcggat gaccgccggc
      541 ggcatgacga gcatcgcgct ggcgggcgcg ctggcgctgc cggacctgcc ggaggccgcc
      601 ggcgtgagtg ccgcgtggcc gctcggtgcg cttctatgcg cgctctacgc cagcctgctc
      661 ctgggctgga ggcgcctcgg ctgaggcgcg tggtgggtga agaccccgcg cttgtgcagc
      721 gtgtggacga gcgagtcgat ggccatgtcg atcgccatct cgagcttcat cccggtgaac
      781 cggccgcgta cgtcgaagcc gagatcctcc agcaccgcga gcacgagctc gcgcgcgtag
      841 tccttcttct cgggcccggt gaggtcgccg aaggcctcgg cctcgtccac cactaggtcc
      901 acgagctgga cgccggcgat gaccgagccg gcgttgttgc cgatccagat cttcaggtcc
      961 gtgatccgct ggagcaggct ctggtggtcg gcgatcatgc cctcgcgctc gatcgcctcg
     1021 agcgcctggc gcagggcggg cgacaggcga acgggatagc gcagcgccat gacgatggcc
     1081 gcggccagcc gtccgaacgg ctccaggacg aacccgacca cacgcctgat cccgcgcagg
     1141 aagcggcggc gcagctcgtg ccaggtcggc cgcgcagtgc gcggcggctc gacccacagc
     1201 ccgcgctggt tggcgacggc cacgacggcg tcgacgctga tgtcggcgac cacgtcgacg
     1261 accaccggct ggaggtagtc gggcacgttg gggatccgga acttgccggt ccgcagcagg
     1321 tagacgacga cggccttgac ctcgtccttc ttgaggtcgc cgcgcccgac cgccccgtag
     1381 cggccctccg cactctgcac gaggacctcg atcaggtcga cccagacgat caccgcctgc
     1441 tggagcgcct cccggttctg gaccagcgcg ttcagcatgt acacgaggtc ctcgtggtcg
     1501 ttgccggcgg tgatctcggc gaacagccgg cggatctcga ggatcgtctc ggccggagga
     1561 aggggcccgc cctcgtcggc gtagcgctcg gggccgcgcc ggtcgaaggc gcgttccatg
     1621 gccgtcgact tgctccgcac catgcggtcc ttgcgggcga gcatcagcgc agccgcctcc
     1681 actcgagcac gagccacgcg tagagaaccg ccagcgccgc gcccagcggc cacgccagcg
     1741 acaccccgag cggctccggg gggctaggga cggtgagcag caccgcgagg ctgacggcgc
     1801 acagcgcgac gacgaggaac cggctgcggc ggttgcgcga gcgctcggcg gccggggtct
     1861 ccgactccca gacgttgagc gagaagctgc cgtcggcgag gtcggacagg atcttctgga
     1921 gctggcccgg cgagtcgcgc agaagcgtca gcacgttcag cgcggagctc tggatccgct
     1981 cggggtcgac cttgcgcgcc ttggcggtga tgtgcgatac gcgcaggtag cggcggctca
     2041 cctcgtcgat gcccgcgtcc gggtcgagcc ggcgagcggt ctcctgcgcg gtgacgatcg
     2101 agcggtagag gagcagcccg ccctccggga gccggacgtg gtgcagccag gcggcgcgca
     2161 tcacgtcggc gagcagttgc cccgcgggcg agggagcgcc gggcgccggg ctgcgcccct
     2221 cccccgaccc gcccaccgcg cgcccgcggg cgaggtcgag cagctcgcgg cggaaggcgg
     2281 cgatgttgcc ctcgcccgcg aacggcgtcg tctcgatcgg cgggtcgagc gcgcgctcca
     2341 tgtcgttctc gaagacggcc gtgaggtagg cgagctggtc ctcggacatc gtcgggtcga
     2401 tctggccgca gtgcgagaag tccacgaacg agatcacgtc gccggggagg gccacgaggt
     2461 tgcgcgggtt caggtcgact ccgaacagcc ggtagcggaa cgcctgccgc aggttcaccg
     2521 tgaggacgac gcgcgcgagc cggaccgggt cgatctcctg ctcgcgcagg acccgcacac
     2581 gaccgggggg ccccgggccg gcggccagga tcacgtcgct catggggacg ccgccgggat
     2641 cctcggagac gagcacggtg ggggcgcaca ggtcggggta cggccgcggg acgaactccc
     2701 aggtgctgtc ggcggcgagc tcccagagcc gctcgaggtt ggcgagctcg ttcgtgaggt
     2761 cgaggtgaag gtcgagccag gcggcgacct cctcggccag gcgcggccgc tcgccgttcc
     2821 cgcggtcccc gagcagcccc gctagcgcgt cggccccgcc gaccgcgcgc gcgaccgcgt
     2881 ccggcgcctc cggccgcaca agcttgatgc gcacgggcgt gccgtccagc aggctgcccc
     2941 tgtgcacctg ggcgatggct tgcgaggcga tcgggcgcgg ctccaccgtc gcgaacagct
     3001 cggacgggtg cgcgccgagc ccctccatca gcaccttccg cacgtccggc cagcccatcg
     3061 gcgggacgtg ctcgccgagg tcggcaagcc gcgtgcgctc gtcggtgtcg agtacgtccg
     3121 ggcgggtggc gagatagcgg gcgatccggc ggagcatggg gtcgaccgac ggtcccgcga
     3181 cgggagccgg cgagccggcc ggcgggtgcg gcgcgggagg gttcggggag gtcatcgcgg
     3241 cgcaagcccc ctcaacgtcg ctgcggtcag cagcagcccg gttgccacgg ccagcagccc
     3301 ggccgtgtgc gcgccggagc cagccacgac gatcagcgcc acgccggcga ccgccgcccc
     3361 gagccagcgc gtctccgcgt gccgggcgcg gcgcgcgctc gtccgctcgc ccgcgaagac
     3421 ggttcccacc aggctcccgc gcgcggcctc gccgatcagc gcggccacgc gatcgggggc
     3481 ctcctgcccg atgccgcgca cgcgcgccca cccgcggcgg tccatcgcga cgcccatcac
     3541 acgctcgatc aggtcagggc ggatctcctc gaagaagcgc cgcagctcct ccatcaggtc
     3601 gtacgtgggc gacatgcgca gcgccgtcga gtcgagcgcg ttcagcgtgc gccagtagag
     3661 caggaagctc atgtcgtaca ggaggcggta gcgcctggag atgtcgatca tctcgccgat
     3721 gtagcggccg aggtggcgct cggcgatggg cgagtcggac cgcagcgaca gctggtacca
     3781 gcgcgtcaga acctcgcgcg cctcggtccg gaacgcccgt acgtcgctct cggccgtgct
     3841 cgcgagctgc cgctcgtaag cgcgcaggct ctcgtcgatg ttgccgatcg ccagctgctc
     3901 gatctgcgcg ccgagcgtgt cgcggtcgaa cccggccagc tcgccgaaga tcccgaagtc
     3961 cacgaacgcg atccggttgt cctcgagcac gagcacgttt ccggggtgtg ggtcgccgtg
     4021 gaagaggccc gtcacgaaga cctgccggag ggtcgcgaac gtcaggtggt gcagcacgag
     4081 atcgacatcg agccacggat actcccgggc cgcgccctcc gccccgtcct gctcgatgac
     4141 ccggatgatc cgcgcgaggc tgtggccgtc gatgaactcg agcgtgagca cgcgcgacgt
     4201 ggtcaggtcc cagtggacgg acggctcgat ctcgtagcgc tcggcgttgc gggcgacgag
     4261 ctcggcggtg cggccctcct gctcgaagtc gatctcgcgg atggtccagt tcgcgaactg
     4321 ctcgagcatc tcggtggcgg acaggcggcc gagcaggtgg agcgcgtcgg cgaggcgggt
     4381 gagccggcgc atgatccgga tgtcggcgga gaagacgcgc tcgattcccg gccgctgaac
     4441 cttgaccgcc acggcgtcgc cgtcccgcgt ccaggcgcgg tgcacttggg cgacggaggc
     4501 ggccgcgagc ggctcgctct cgaaacgagc gaagcgctcg tcgatcggcg caccgagctc
     4561 ctcctcgacg atcgagcgcg ccacctcggc cggcagcggc gccacctcgt cgaacaggcg
     4621 cccgagctcg cggcagacct cgggcgggag gatgtcgaag cgcatcgcga ggtactggcc
     4681 gagcttgagg taggtgagcc cgaggtcctc gagcgcctcg cggatctgca ccggcaggcc
     4741 cggcccgcgc agcggcagga accgcccggc gaccatccgc gtcccgaagc gggcgacagt
     4801 ccacgccagc tcgagggcgc gccgcacctc gaggacgcga tccctcagcc gcacggcacc
     4861 cggctcgcga ggtcgtgctc gatcgcgcgc ataagctgct cgggcgcgcg ggccgcgaag
     4921 aagtggttac ccgggatcag ccgaagccgg aagggaccgc gggtgaggtc ggcccagggg
     4981 ccgatcgcgc acaccggcac ctcggggtcg ctcgtggcgc cgaacgccga gatcgggatc
     5041 gtgaggaagc ggccggcggt cgggtcgtac acgtagtcgg cgcagagccc gagatcggcg
     5101 cgcacgatcg ggaggaagtg gtccatcagg gcggtgttgt ccagcaccgc cgacggcgtg
     5161 ccgcccatcg cgcccagccg ggcgaccagc tcgtcgcgcg ggagcaggtg ggtcgccggc
     5221 acgtcgccgt gcaggtgcgg cgcgacgtgg gcggccacga acaggtgcac gggcggcgcg
     5281 gcgcggtcgc cgagccgccg cgcc
//
LOCUS       DASVPL010000193         8133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_178968, whole genome shotgun sequence.
ACCESSION   DASVPL010000193 DASVPL010000000
VERSION     DASVPL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178968"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VF517_10045"
     CDS             <1..1313
                     /locus_tag="VF517_10045"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX8053324.1"
                     /translation="EFDIGWAPELEGEFEGVERDLDVTAVDPATAGRNAELCRIGAEA
                     LGAANRPLPRAAGRVACCGTCPMGCALDAKRAMHVSELPRAAAAGARIVAGARVTRVL
                     VEGGRAAGVEACAAAGAGVAGAGQDAAGAAGAGPDAAGAAGAGADAAGAAGARPGAEA
                     RAAGVEAGAGAAGAPFRVDARAVVLAAGALGTPELLLRQGIAGRSGHLGRHLRVQPAC
                     WVGARHPGEAVRGWDGVMQSWGVGEWADRGLFLEATFSPLPFGAHWMPGAGPAFAERV
                     ERYGEMAVVGVHLADRSEGRVRVRGGQLRVAYRLTRDDAATLAFGIARAAAIHFASGA
                     GEVYPQVAGAGILRPGEERGIEERRVRPAELRLEAFHPMGTARMGDEPSGSVVGPDGE
                     VHDVPGLFVADASVLPTALGANPMLTIMAVARHTARGLAERLAY"
     gene            complement(1327..2583)
                     /locus_tag="VF517_10050"
     CDS             complement(1327..2583)
                     /locus_tag="VF517_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053325.1"
                     /translation="MTTGAPAAEAEGREPDDAGGLRGRGGWHLVAFLAALALVLAMTI
                     DWYTTEQGEEFRRVEQVNRDREDSQRLDSEDVERAAQAAENEERNAFQADAFIDRLIL
                     LACLAAFVAAVATAFLRSMRRRAEPPWNPSALATVAGILATLLILYRMIQPPGFNEAA
                     VVKPGVPIALAAVGILTVAARLATLIEREERLGPVAVAAAEAEAAPRRRGRGRRSASV
                     SSVTPVSLASPEAEAAPRRRGRGRRSRQEPAPGLAQPLAAPEAAQGSARSATPGPAPG
                     SEWPAVPDSARPAAPGPAQGSDRPAESGSAWPPAAAAGPPAPEPERAPGSLWAPPPRD
                     PESAAGAAGDPFGPAEDPSAEAREARRRAREQARAARSGLGGDADSERREDHAVELSF
                     DADPPDPTRRADTPPEPPPAGPPTER"
     gene            complement(2580..3116)
                     /locus_tag="VF517_10055"
     CDS             complement(2580..3116)
                     /locus_tag="VF517_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053326.1"
                     /translation="MNLRRLRIGEWIVGIGGVLLLATTFLDWYDVESRRPVEAMHSAG
                     VMVAEGSLSAWEAFSVIDVLIALLAVLAIGAAVMAAAHNTPAVSLAMASMSVWIGLLT
                     LIALLVRAFALPDFALDGFVIPDDDVSVAPGLWLGLGACALTVAGAFASIRDERFPRA
                     ARIEVPVETIPPPEGGTA"
     gene            complement(3136..6477)
                     /locus_tag="VF517_10060"
     CDS             complement(3136..6477)
                     /locus_tag="VF517_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX8053327.1"
                     /translation="MSATAARDRLLEDLNEPQREAVIYDEGPLLVLAGAGSGKTRVLT
                     HRIAYAVETDRAAPGEILAITFTNKAAKEMRERVEMLVGRRSRVMWVMTFHSACARML
                     RAEGPRLGYTRGFTIYDEADSVRLVKHCIEALDVDPKRFTPRAIKRQISDAKNTLLDA
                     DGYRQKVGSFFEQTAADVYELYEKRIHSMNAMDFDDLLVRCVDLLQLFPEVRERYQQS
                     FRMVLVDEYQDTNRAQYRWLQLLASEHRNLCVVGDDDQCLVEGTPVTMVDGTTKPIES
                     IVEGDEVLSSYGGGDFRGARVTATTRRLADELIAITTASGRRLVSTPEHVHFAGYHRA
                     VTPPLHMTYLMWRRDKGFRLGTSRSRPDSRWAPVSGVHLRSVQEHADAAWVLSTHDSE
                     AAARAEEMKLSLQYGIPTLPFVARKGKSVNGLVCDQALIDDVFRSTDARDAGRWLLDD
                     LGLSFGHPHHVPATHEGRRRNVTVTLCADRRGRTAMHTIAMGGRDPGVARRLRDAGLN
                     VRPARRGSSSWRYESSFKDFDAGVRVLNRIQAATPVNVRCKARLGTAAGEAKQHNSLP
                     FMPAASVRPGMAMFAEDGSYDVVTSVERVWEERPVYDLNVEGTHNFVAEGLVTHNSIY
                     AFRGADLRNILDFEDDYRDARVIKLEQNYRSTQNILSAANAVVSNNRARKHKALWTDA
                     GEGEPVRVAELEDEHAEARYVTDRIERLVEEGGSRSEVAVFYRTNAQSRVLEDMLVRF
                     NVSYQVIGGTRFYERAEVKDAIAYLTLLVNPQDVVAFERVVNSPRRGIGNTTQARIVS
                     QANTLGEAVWDVALDPAAVPGLGAAAIKAVGRFMSTMERLREWMGSASVGDLLQQVLD
                     ETGYLEALRAERTIEAEGRIENLEELVSVAREYDGTAEEPSVEEFLQQIALFSEQDNL
                     TDDEGVVTLMTLHNAKGLEFPVVFILGCEDGVFPHSRAVESGDLEEERRLCYVGITRA
                     RRQLYMTFARTRSLYGGREWNMPSRFLDELPDELVEREAQAPQRYQQTWAARESVGAG
                     SRFGGGSGGIYGRSGTGAGATPQRREPVQGGGASFSLGDDVVHATLGEGVVTGIEPGG
                     LVVVRFAADGSERKLMSDYAPLKKR"
     gene            6504..7367
                     /locus_tag="VF517_10065"
     CDS             6504..7367
                     /locus_tag="VF517_10065"
                     /inference="COORDINATES: protein motif:HMM:NF021832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HEX8053328.1"
                     /translation="MGLCDEVRRSAAAIVARSEHVRIDLDRLRAIEPGPPPVLDPERH
                     YLEGSREEVALYILTLDSINFGSGWFPTLRKRPGSSGYYTVAWALADRFRAHGPWSPD
                     ELRALSPAAVADTLGQDRDHELMALYARALNDLGAWLGERAALDVIGDSAEDLAEELA
                     AGMPFFDDRGFWKRAQITPNDLALAGIAEFGDIDRLTIFADNLVPHVLRMDGVLRYDD
                     ALATRIDAGELLEPGGAEREIRAAALHACELIAAELGVAPRNLDVMLWNRGQEPRYKA
                     VPRHRTRTVFY"
     gene            7403..8122
                     /locus_tag="VF517_10070"
     CDS             7403..8122
                     /locus_tag="VF517_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053329.1"
                     /translation="MKKTIAIAATAALAAGGVGAVAATKTKDATSRCVVNGAETVTAN
                     NDLRVYALDKGFKDDSSATGLYACRYSTGKRVSLGTAYVSNDDEGGSGPIRYIRDISL
                     SEDLNDGSAPAVAFVDTNCVKNPCKSEVVVKSMVTGKTTRRFKAGSPFDYLELSFPTD
                     QDGLAIAWLETSAGGTCEQGCRVHLIKESGDKVLDEGTDIDADTFGALANDHPGVIHS
                     LGTNEFVWKRGATVKIASFND"
BASE COUNT         1085 a         2989 c         2953 g         1106 t
ORIGIN      
        1 acgagttcga catcggctgg gcgccggagc tcgagggcga gttcgagggg gtcgagcgcg
       61 acctcgacgt gaccgcggtc gacccggcca ccgccgggcg caacgctgag ctatgccgca
      121 tcggcgcgga ggcgctcggc gcggcgaacc ggccgctgcc gcgcgcggcg ggccgggtcg
      181 cctgctgcgg cacgtgcccg atgggctgcg cgctcgacgc gaagcgagcc atgcacgtgt
      241 ccgagctgcc ccgcgccgcg gcggctggtg cgcggatcgt ggcgggagcg cgcgtgaccc
      301 gcgtcctggt ggagggcggg cgcgcggccg gggtggaggc atgcgcggcg gcgggcgcgg
      361 gcgtggcggg cgcaggccag gacgcagcgg gcgcggcggg cgcaggcccg gacgcagcgg
      421 gcgcggcggg cgcaggcgcg gacgcagcgg gcgcggcggg cgcacgcccg ggcgcggagg
      481 cgcgcgcggc gggggtggag gcgggcgcgg gcgcggcggg cgcgccgttc cgcgtggacg
      541 cgcgtgcggt cgtgctcgcc gccggggcgc tcggcacgcc cgagctgctg ctgcgccagg
      601 gcatcgcggg gcgctccggg cacctcggcc ggcacctgcg agtgcagccg gcctgctggg
      661 tcggcgcgcg gcatcccggc gaggccgtgc gcggctggga cggggtgatg cagtcgtggg
      721 gcgtcggcga gtgggccgat cgcggcctgt tcctcgaggc caccttctcg ccgctcccgt
      781 tcggggcgca ctggatgccg ggcgcggggc cggcgttcgc ggagcgggtg gagcgctacg
      841 gcgagatggc ggtggtcggc gtgcacctgg ccgatcgctc ggagggtcgg gtgcgcgtgc
      901 gcggcgggca gctccgcgtc gcgtaccggc tcacgcgcga cgatgccgcc accctcgcct
      961 tcgggatcgc gcgcgcggca gccatccact tcgcctcagg agccggcgag gtctacccgc
     1021 aggtcgccgg cgcggggatt ctgcgaccgg gcgaggagcg cgggatcgag gagcggcggg
     1081 tgcggcccgc cgagctgcgg ctcgaggcat tccacccgat gggcacggcg cggatgggcg
     1141 acgagccgtc cgggtccgtc gtggggccgg acggcgaggt gcacgacgtg ccggggctgt
     1201 tcgtggccga cgcgagcgtc ctccccaccg cgctcggagc gaacccgatg ctgacgatca
     1261 tggccgtcgc gcggcacacc gcgcgcggcc tggccgagcg actggcctac taggagagcc
     1321 ggggcgctac cgctcggtgg gcgggccggc cggcggaggc tccggcggtg tatccgcgcg
     1381 gcgggtcgga tcgggtgggt ccgcgtcgaa cgagagctcc acggcgtggt cctcgcgccg
     1441 ctccgaatcg gcgtcgccgc ccaggcccga gcgggcggcg cgggcctgct cgcgcgcccg
     1501 gcgccgcgcc tcgcgcgcct ctgcgctcgg atcctcggcg ggtccgaacg ggtcgccggc
     1561 ggcaccggcg gccgactccg ggtcgcgcgg cggcggagcc cagagcgagc ccggggcccg
     1621 ttcgggctcg ggcgcaggcg gcccggccgc cgcggccggc ggccacgcgg agccggactc
     1681 ggcgggccgg tcggagccct gtgcgggtcc cggcgcggcg ggccgcgcgg agtcgggcac
     1741 ggcgggccac tctgagccgg gtgcaggccc cggcgtggcg gatcgcgcgg agccctgcgc
     1801 ggcctccggc gcggccaggg gctgcgccag gccgggtgcg ggctcctggc gcgagcgtcg
     1861 cccgcgcccg cggcgccgcg gcgccgcctc ggcctcgggt gacgccagtg aaacgggtgt
     1921 aacggatgaa acggatgcag agcgacgccc ccgcccccgg cgccgcggcg ccgcctcggc
     1981 ctccgccgcc gccacggcga ccggaccgag ccgctcctcg cgctcgatca gcgtcgccag
     2041 ccgcgccgcc accgtgagga tcccgaccgc cgccagcgcg atcggcacgc ccggcttgac
     2101 caccgcggcc tcgttgaagc cgggcggctg gatcatccgg tagaggatca gcagcgtggc
     2161 gaggatgccg gccacggtcg ccagcgcgct cgggttccag ggcggctccg ccctccttcg
     2221 catcgaccgc aggaacgccg tggccaccgc cgccacgaat gccgcgagac acgcgagcag
     2281 gatcagccgg tcgatgaacg cgtccgcctg gaacgcgttg cgctcctcgt tctcggccgc
     2341 ctgcgcagcc cgctcgacgt cctcgctgtc gagccgctgc gagtcctcgc gatcacgatt
     2401 gacctgctcg acccgccgga actcctcgcc ctgttcggtc gtgtaccagt cgatcgtcat
     2461 cgcgagcacc agcgccagcg ccgccaggaa cgcgacgagg tgccagccgc cgcgccccct
     2521 cagcccgccc gcgtcgtccg gctcgcgccc ctcggcctcg gccgcgggcg cgccggtggt
     2581 caagccgtcc cgccctcggg cggcgggatc gtctcgacgg gcacctcgat ccgcgctgcc
     2641 cgcgggaagc gctcgtcccg gatgctcgcg aacgcgccgg cgacggtgag cgcgcaggct
     2701 ccgagtccca gccacagccc gggcgcgacg gagacgtcgt cgtcggggat gacgaagccg
     2761 tcgagcgcga agtccggcag cgcgaacgcg cgcacgagca gcgcgatcag ggttaagaga
     2821 ccgatccaaa ccgacatcga cgccatagcc agcgagaccg cgggcgtgtt gtgcgccgcg
     2881 gccatcacgg cggcgccgat ggcgagcacc gcgagcaggg cgatcagcac gtcgatcacg
     2941 gagaacgcct cccacgccga cagcgacccc tcggcgacca tgacgcccgc cgagtgcatg
     3001 gcctccaccg gccgccggga ctcgacgtcg taccagtcga ggaacgtcgt cgcgagcagc
     3061 agcacgccgc cgatgccgac gatccattcg ccgatgcgta gccggcggag gttcacgcgc
     3121 ggcagggtag cgccgtcagc gcttcttcag gggcgcgtag tcggacatga gcttgcgctc
     3181 cgagccgtcg gccgcgaagc gcaccaccac gagcccgccg ggctcgatgc cggtcacgac
     3241 gccctcgccg agcgtggcat gcaccacgtc gtcgccgagc gagaacgacg ccccgccgcc
     3301 ctgcaccggc tcgcgccgct gcggcgtggc ccccgccccg gtgccgctgc gcccgtagat
     3361 gccgccgctc ccgccgccga accgcgaccc ggcgcccacg ctctcgcgcg cggcccacgt
     3421 ctgctggtag cgctgcggag cctgcgcctc gcgctccacg agctcgtccg gcagctcgtc
     3481 gaggaagcgg ctcggcatgt tccactcgcg ccccccgtat agggagcgcg tgcgtgcgaa
     3541 ggtcatgtag agctgccgcc gcgcccgcgt gatgccgacg tagcagagcc gccgctcctc
     3601 ctcgaggtca cccgactcga ccgcccgcga gtgcgggaag acgccgtcct cgcagccgag
     3661 gatgaacacg accgggaact caaggccctt ggcgttgtgg agggtcatga gcgtgacgac
     3721 gccctcgtcg tcggtcaggt tgtcctgctc ggagaagagc gcgatctgct ggaggaactc
     3781 ctcgaccgac ggctcctccg ccgtgccgtc gtactcgcgc gcgacgctca ccagctcctc
     3841 gaggttctcg atccgcccct cggcctcgat cgtccgctcc gcccgcagcg cctccaggta
     3901 gcccgtctcg tcgagcacct gctggagcag gtcgccgacc gacgcgctcc ccatccactc
     3961 gcgcagccgc tccattgtgg acatgaagcg gcccacggcc ttgatcgcgg ccgcgcccag
     4021 gccgggaacg gcggccgggt cgagcgccac gtcccagacg gcctcgccca gcgtgttcgc
     4081 ctggctcacg atccgcgcct gggtggtgtt gccgatcccg cgccgcggcg agttgaccac
     4141 gcgctcgaac gcaaccacgt cctgcgggtt cacgagcagc gtgaggtacg cgatcgcgtc
     4201 cttcacctcg gctcgctcgt agaagcgcgt gccgccgatc acctggtagc tgacgttgaa
     4261 gcgcaccagc atgtcctcga gcacgcgcga ctgcgcgttg gtccggtaga agaccgccac
     4321 ctcgctgcgc gacccgccct cctcgaccag gcgctcgatc cggtcggtca cgtagcgcgc
     4381 ctcggcgtgc tcgtcctcca gctccgccac ccgcaccggc tcgccctcgc ccgcgtcggt
     4441 ccagagcgcc ttgtgcttgc gggcgcggtt gttggagacg accgcgttgg ccgcgctgag
     4501 gatgttctgc gtcgagcggt agttctgctc gagcttgatg acccgagcgt cccggtagtc
     4561 gtcctcgaag tcgaggatgt tgcggaggtc ggccccgcgg aaggcgtaga tcgagttgtg
     4621 cgtgacgaga ccctccgcca cgaagttgtg ggtcccctcg acgttgaggt cgtagaccgg
     4681 gcgctcttcc cagacccgct ccacgctcgt gacgacgtcg tacgacccgt cctcggcgaa
     4741 catggccatc ccgggccgca cggaggccgc cggcatgaat ggcagcgagt tgtgctgctt
     4801 ggcctcgccc gccgccgtac ccagcctcgc cttgcagcgg acgttcaccg gggtcgccgc
     4861 ctggatgcga ttcaggacgc gaacgcctgc gtcgaagtcc ttgaaggaac tctcgtaacg
     4921 ccagctcgac gagccgcgcc gggcggggcg cacgtttagg ccggcgtccc tcagccggcg
     4981 agccaccccg ggatcgcgcc cgcccatcgc gatcgtgtgc atcgcagtcc ggccgcgccg
     5041 gtcggcacac agcgtcactg tgacgttccg acgcctgccc tcgtgcgtcg ccgggacgtg
     5101 gtggggatgc ccgaagctca ggccgaggtc gtcgaggagc caccgcccgg catcgcgtgc
     5161 gtccgtgctg cgaaagacgt cgtcgataag tgcttggtcg caaaccagcc cgttgacgct
     5221 ctttcccttc cgagcgacga acggaagcgt gggaatgccg tactggagcg acagcttcat
     5281 ctcctccgct cgcgcggcgg cctcgctgtc gtgggtcgat aggacccaag cggcgtcggc
     5341 gtgctcctgg accgatcgca gatgcactcc gctgacgggc gcccagcgac tgtcggggcg
     5401 gcttcggctc gtgccgagcc ggaaaccctt gtcacggcgc cacatgaggt aggtcatgtg
     5461 cagcgggggc gtgacggcgc gatggtaacc ggcgaagtga acgtgctccg gcgtgctcac
     5521 gagccgccga cccgaggccg tggtgattgc gatcagctcg tccgcgagtc gccgggtggt
     5581 cgcggtcacc ctcgccccgc gaaagtctcc acccccgtag gacgacagga cctcgtctcc
     5641 ctcgacgatc gactcgatgg gcttcgtcgt cccgtcgacc atcgttaccg gggtcccctc
     5701 gacgagacac tggtcgtcgt ccccgacgac gcacaggttg cggtgctcgc tcgccaggag
     5761 ctggagccag cgatactggg cgcggttggt gtcctggtac tcgtcgacga gcaccatgcg
     5821 gaacgactgc tggtagcgct cgcggacctc ggggaagagc tggagcaggt ccacgcagcg
     5881 gaccagcagg tcgtcgaagt ccatcgcgtt catcgagtgg atccgcttct cgtagagctc
     5941 gtagacgtcg gcggcggtct gctcgaagaa cgagccgacc ttctgccggt agccgtcggc
     6001 gtcgagcagc gtgttcttcg cgtcggagat ctggcgcttg atcgcgcgcg gcgtgaagcg
     6061 cttggggtcc acgtcgagcg cctcgatgca gtgcttcacg agccgcaccg agtcggcctc
     6121 gtcgtagatc gtgaacccgc gcgtgtagcc cagccgcggc ccctcggcgc gcagcatccg
     6181 cgcgcaggcg gagtggaagg tcatcaccca catcacccgc gagcggcgtc ccaccagcat
     6241 ctccacgcgc tcgcgcatct ccttcgcggc cttgttcgtg aaggtgatcg cgaggatctc
     6301 gcccggcgcc gcgcggtcgg tctccacggc gtacgcgatc cggtgcgtga gcacgcgggt
     6361 cttgccgctg cccgccccgg cgagcacgag cagggggccc tcgtcgtaga tcacggcctc
     6421 gcgctggggc tcgttcaggt cctccaggag gcggtcgcgg gcggcggtcg cggacactcc
     6481 gtcgaggtta gcctcagccc ccgatgggac tgtgcgacga ggtccgccgc tccgccgccg
     6541 cgatcgtggc gcgcagcgag cacgtgcgga tcgacctcga ccgcctgcgc gccatcgagc
     6601 ctgggccgcc gcccgtgctc gacccggagc gccactacct ggagggctcg cgcgaggagg
     6661 tagcgctcta catcctcacg ctcgactcga tcaacttcgg gtcgggctgg ttcccgacgc
     6721 tccgcaagcg gccgggctcg tccggctact acacggtcgc ctgggcgctg gccgaccgct
     6781 tccgagcgca cgggccgtgg agccccgatg agctgcgcgc tctctccccc gccgccgtcg
     6841 cggacacgct gggccaggac cgcgaccacg agctgatggc cctctacgcg cgggccctga
     6901 acgacctcgg cgcctggctc ggggagcgcg cggcgctgga cgtgatcggc gactccgccg
     6961 aggatctcgc cgaggagctc gccgccggga tgccgttctt cgacgaccgc ggcttctgga
     7021 agcgcgcgca gatcacgccg aacgacctcg cgctggccgg catcgccgag ttcggcgaca
     7081 tcgaccggct cacgatcttc gccgacaacc tcgtccccca cgtcctgcgc atggacggcg
     7141 tgctccgcta cgacgacgcg ctcgccaccc ggatcgacgc cggggagctg ctcgagcccg
     7201 gcggcgccga gcgcgagata cgcgcggccg ccctccacgc ctgcgagctg atcgccgccg
     7261 agctcggcgt ggcgccgcga aacctcgacg tgatgctctg gaaccggggc caggagccgc
     7321 gctacaaggc cgtcccgcgc caccgaacgc ggaccgtctt ctactagcgc aaccctcggc
     7381 cggcgggcgg gttagcgctc gcatgaagaa gacgatcgcc atcgcggcca ccgccgccct
     7441 cgccgctggc ggcgtgggtg ccgtcgccgc caccaagacg aaggacgcga ccagccgctg
     7501 tgtcgtgaac ggtgcggaga cggtgaccgc gaacaacgac ctgcgcgtgt acgcgctcga
     7561 caaggggttc aaggacgact cgtccgccac cgggctgtac gcgtgccgct actccaccgg
     7621 caagcgcgtc tcgctgggca ccgcctacgt gtcgaacgac gacgagggcg gctccggccc
     7681 gatccgctac atccgcgaca taagcctctc cgaggacctg aacgacggca gcgcgcccgc
     7741 ggtcgcattc gtggacacga actgcgtcaa gaacccgtgt aagtccgagg tcgtagtcaa
     7801 gtccatggtc accggcaaga cgacgcgccg gttcaaggcc ggcagcccgt tcgactatct
     7861 cgagctgtcg ttccccaccg accaggacgg gctcgcgatc gcgtggctcg agacctcggc
     7921 cggcggcacc tgcgagcagg gctgccgggt tcacctgatc aaggagtccg gcgacaaggt
     7981 gctcgacgag ggcaccgaca tcgacgcgga cacattcggg gcgctggcga acgaccatcc
     8041 gggcgtgatc cactcgctcg ggacgaacga gttcgtctgg aagcgcggcg ccaccgtgaa
     8101 gatcgccagc ttcaacgact agaggcgcgt gcg
//
LOCUS       DASVPL010000194        17462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_179148, whole genome shotgun sequence.
ACCESSION   DASVPL010000194 DASVPL010000000
VERSION     DASVPL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17462
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179148"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VF517_10075"
     CDS             complement(<1..415)
                     /locus_tag="VF517_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8053330.1"
                     /translation="MTHPAAAGDLTHLLEVAREAAGAGAAAAMSWWHDLDRLSVGEKA
                     ASDDLVSQADRDAEDAIRAVLSRHRPGDGVVGEEGGSTRGESGVEWMVDPIDGTTNYL
                     YGRPDWAVSVAAVRADGGAVIAGVVAEPALDRLTEA"
     gene            complement(412..1641)
                     /locus_tag="VF517_10080"
     CDS             complement(412..1641)
                     /locus_tag="VF517_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053331.1"
                     /translation="MSDSAPGRSLPSYLLLPRPKDLGKAWILPAAFGLGVLCDGGASA
                     HELLRAAVVWACLELLIYQARYQWNDIRGFAADQAHPDAASRGRLPGPVERARPHIAA
                     SALAGVARLAIAAAVAVGISGLDAGGVLLVLGVAVFAVAGVYESLRAAATGRTDAVPP
                     PLRPTLVALWIAVGGGYAVRGVGGLALAVDLTGEPALLVAAVVTMWAFGVAFVTARWA
                     VEALAFARLDGPWVVWRAHAGQAREHLLALVRWLPARVPAGPAGRAPVPLRDWAALSE
                     PTPRAAPWNVAILVAGAAAALTGRLLVDSSGDLLAPAAGLLLALGAVAAPAGWRWASV
                     AAGAAALAAVLALAGTERPLLAALPWLAVLGSHAFFSGQSLTTVGRGLRGRLAARAAA
                     GSPRRTPRQGALQSSHR"
     gene            complement(1638..2417)
                     /locus_tag="VF517_10085"
     CDS             complement(1638..2417)
                     /locus_tag="VF517_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX8053332.1"
                     /translation="MGNLPLEPAAVQVLLCDADGNLFPSEEPAFEASAVVTNRMMEAI
                     GSTVRFDPVDLRLRTTGLNFRSTAGDLAVAAGRPLPSHELEHWVEEEARAVTAHLARV
                     LKPNRSVTRPLERLARDLELAAVSSSALARLAACFEATGLAELLPPGRRFSAEDSLPV
                     PRSKPDPAIYAHALAELGVPARRALAVEDSVPGARSALAAGIPTVGNVMFVPGDERAA
                     RRAALAEAGAAAVVSSWVELERLLMPRPRLSERRDAAGASA"
     gene            complement(2417..3910)
                     /locus_tag="VF517_10090"
     CDS             complement(2417..3910)
                     /locus_tag="VF517_10090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX8053333.1"
                     /translation="MPNARPLAASTLHAHGARVRVPTYDRRALRPGVVHLGVGGFHRA
                     HQATYFDDLAQDGVTMDWGIVGVGMRRPHMQDALAPQDCLYTVVERDEGASSARVVGS
                     MPRYLFGPADPEAVFGALTDSRTRVVMLTVTGDGYHVDAATGELDADGEAIRADLANP
                     SRPTTPIGYIVEALARRRAAGVPPFSVVSCDNIPKNGDVTRAALVGFGTMRDELLGRW
                     IETRVACPSSMVDRITPETTAADRAAIRREFGVDDRWPVVTEPFRQWILEDEFCDVRP
                     PLEEAGVQFVSDVAPYQLMKTRLLNASHSALGYLGSLAGHVRTDDAMADPLLEAYVTD
                     LMAREVAPLLPATPGVDRDEYQSTLLQRFANPMIGDPLARLCGRGSTKMPAYLLPSIA
                     DSQKRGRSHPLLTLAVAAWLRYLRGSDCAGQRIEVKDALVDQLQPLARAGGSDPRPLL
                     ARRDLFGDLGQNASFVAAVRRDLMTLDRDGVHAAVAGHLDARAARAA"
     gene            complement(4120..5775)
                     /locus_tag="VF517_10095"
     CDS             complement(4120..5775)
                     /locus_tag="VF517_10095"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX8053334.1"
                     /translation="MTNGARALLGTLVDGGVTTCFTNPGTSEMHFVAALDSVPEMRAV
                     LALFEGVATGAADGYSRMAGTPAATLLHLGSGLGNGLANLHNARTGRVPIVNIVGDHA
                     THHTQYDAQLQSDIETVARNVSSWVRTSRSTAELGRDAAEAIAVAKGPPGQVATLILP
                     ADVSWSEGGEPAPAAAPAAPPVAPAEVVEEAVSALRGGEDTAILLGGRALREPALVAA
                     GRVAAATGAKLLAEVFPTRLQRGAGIPPIERVAYLAELASVQLAGLKHLILVDAKAPV
                     SFFAYPGKKSYLVPDGCEVHELAASDQDALGGLEALVDALGAGAAEPVRQAASRPARP
                     TGQLDADKVCQAIGAVLPEGAIVSDESQTSGVTLATHTAGAPPHDLLALTGGAIGQGL
                     PVALGAAIACPGRPVFALEGEGSAMYTIQSLWTMAREELDVTVVIFNNRSYAILNIEL
                     ERVGAERPGPRAKSQLDLAGPDLDFVRIANGLGVPAVRTETGEELVTALEQAVAEPGP
                     HLVEAVVPSVYSGLRLRAMPHALNVLGKLPRPVAKAAKARFYP"
     gene            5900..7138
                     /locus_tag="VF517_10100"
     CDS             5900..7138
                     /locus_tag="VF517_10100"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF005734.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX8053335.1"
                     /translation="MSLQELRSPSATDQPEPGDQPLGVLPAQGLRGAIDKGWISGGDY
                     AIRPASVQPASLDLRLGDRAWPLRCSFLPDPKSTVMSRLHDLALEEIDISKGATLERD
                     RAYLIPLVEELALPGEVRAKANPKSSTGRLDVFTRVITDGNHRFDEIPKGYRGKLFLE
                     VVPRSFAVKVEKLLSLNQLRLAVGDPRVRDDDLRRAHQEFPLLYVGERKVPDEDLAVS
                     DGLFLSVDLSGPPDRIVGYRAKKNSHRIDLSQIRHYRWRDYWEPVYPERGGRIVLEPE
                     IFYLLLSSERVCVPPEYAAEMMAYDPTAGELRTHYAGFFDPGFGYEDTGGKFGSRAAL
                     EVRARDVAFMVEHGQPICKLAFERMLEPPDEHYGSAVGSNYQGQETMLSKHFVEQGLG
                     RAQAGAGAAEVDAATNPLFE"
     gene            7143..7697
                     /locus_tag="VF517_10105"
     CDS             7143..7697
                     /locus_tag="VF517_10105"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX8053336.1"
                     /translation="MALWLVIAVAVLLVVGVALIYNSLVAKRNRAENAWAQVDVQLRR
                     RHDLIPNLVETVKGYAAHEKGTFEAVTETRAAAQQAQGPAAQAQAETALSGALGRLMA
                     VAENYPALRATENFQQLQGELGSTENRIAVARQVYNDTVLTLNNACQQVPTNIVANAF
                     GFRLREYFTVDAGDPARDPVSVGF"
     gene            7697..9586
                     /locus_tag="VF517_10110"
     CDS             7697..9586
                     /locus_tag="VF517_10110"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX8053337.1"
                     /translation="MRRAPAALAAALLVLLLAAAPAGAKSFALSDADVDVRVAPDGGI
                     VVRESITFDYAGSFSGAFRDIPLRKGESIDRVSVAERGERYKPGGSLTLGTGGPASQF
                     GSGVVDGKQRIVWRFRALDERRTYTIRYRISGLALAYDDVVDVNLQVWGSTWETGLAR
                     LRATMAAPREVTARNARVFGHPVTVPGDTAFGGGAAHLRALDVPKQQFVEMRLLFPRS
                     MLTSTKRAKVARGEARTRILAAERADAADYEHNRRKIDDAVDHLPANLLLLLFLGVAP
                     AAAILALVWLVLSRERRAADYDREYEQEPPSDDPPALIAPLLRQSTKPGGEEFTATLF
                     DLIRRGYLTTEPTTTEKWSWGALGKREGPDLLLSRKGATGDAAPLEKGVAKILERALG
                     NGPLPLSELSEKIKGNREANANSYSSWQEKIGKEFGRRHWYDDRAAKVIGAAAAAMGL
                     AAIALIAIGVVGIDSRFVRWGEVVQIALGVAAAINAVVLVVSLRNERLRRRWTPAAAL
                     EAARWAAFRRYLRDFPRLQEAPPESVALWERLLVYGIAFGLADRVLQAAKIAAPEALA
                     QSGIYAPGYYAGGETFYSGSSISSVSSGFSSALAPPSSGSDGGGGGFSGGGGGGGGGG
                     GGGAW"
     gene            9583..10389
                     /locus_tag="VF517_10115"
     CDS             9583..10389
                     /locus_tag="VF517_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8053338.1"
                     /translation="MSPLREINGVRLLVEESGSGPALVLVHGSWDDRRSWGRIENELS
                     RRYRVVSYDRRGHTGSDDGYPAGTRRTDEDDLAEIIEALGLAPAHVVASSFGGSISLG
                     LAARRPELVQTVCAHEPPLLSIAADDPEVARVFETMRSLLELIDRGEREAAARGFADD
                     IALGRGSWAMIPTDVRASMVANAETAAGEVRDPEWAQLDLDGLSRAGVPLLVTQGNTS
                     PLFFRRIIERLAAAVEGAEVRTLPGAGHLPHLTHPAEYVAAVRGFIERSA"
     gene            complement(10370..11572)
                     /locus_tag="VF517_10120"
     CDS             complement(10370..11572)
                     /locus_tag="VF517_10120"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF008398.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEX8053339.1"
                     /translation="MTRYRADRPLGVAPATAADYRELARRRLPRQLFDYVDGGAYDES
                     TMRANVSDLGRVLLRQVVMRDVSVREQAVEVLGQQLALPIVLAPVGLAGMCAPRAEVL
                     AARAAEAAGVPFVESTVSICSIEEVAGATTAPPWFQLYVMRDRGYAQDLMARAQAVGS
                     PVLMLTIDLAVVGARHRDTRNAMVGEHTAWAKARRGLDLVSHPRWLREVAVGGKPLTF
                     GNLENAVPDASTPADFKQWVDEQFDPSVTWDDIAWVREHWNGRLVVKGVLDPEDARRA
                     ADAGVDGVVVSNHGGRQLDSVPSTARALPDVVDAIGDEVEVLVDGGVRTGLDVVKMVA
                     LGARAVLIGRPWAWAVAARGQAGVRHVIEGMRADIDTALGLTGQNSVAGLDRSALYEG
                     RGLKPSAR"
     gene            11728..13095
                     /locus_tag="VF517_10125"
     CDS             11728..13095
                     /locus_tag="VF517_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8053340.1"
                     /translation="MIKAGTRRAIAASIAVVAAAAVPAPALAAEAHVETIDGVKVIVF
                     NAAPGELQSVLVDRDPGFTNDQYQLEDQNNKVSAGTGCANPPRTPFAGVEEKIVRCDA
                     SATTIRVNLADGRDSVRFGDLSGHLITDVVDGGDGDDSLDTSNGSGLIMGGPGDDTID
                     ANAGDDEVIGGDDDDTIVGGPGSDTISGDAGDDKIRGHATPDTHEIGKVTDFRAGDVN
                     KLAGGPGNDTIVGDNGTDELTGDTGNDNLNGAGAADLLDGGAGNDTIDEGDTPGDPNG
                     GGPAGPLAPDTVRGGSGTDTATYCTRHYSDSEARKHTLTITLDDKANDGEKGEKDQIG
                     SKGDVENVLGGGVTNDRITGNSRANELIGDCLSTFTSSGDNKLYGAGGNDKLVGSSGR
                     DLLNGGKGADRFLGNEDADTIQAKDGARDKSINCDGLGAKSSKDSATVDRSDPTPFNC
                     DKLRR"
     gene            complement(13104..13973)
                     /locus_tag="VF517_10130"
     CDS             complement(13104..13973)
                     /locus_tag="VF517_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8053341.1"
                     /translation="MRDAWAVIKRVLFSFYDDQATHHAAALTYYALMSLFPTLLLGVS
                     LLGLLGEYPGTYDSIVDYLRDVVPATTLAPIDDAVRAALRSKGSAALALLISTLTVLY
                     GTTGYLEAARRAMNVVFEAGDGRSFLRRKLTDVMSTVVLFALVLATLVLVFVGAEAAE
                     DLAGSTAASVWMVVRWPLALASAMLVFSFLYYVTPDVKQRAFRWLTPGAAAGVAVWIA
                     ASAAFSEYIGHFSSINVTYGSFAAAIILLFWLWLTNVALLFGAEVNAEIEREKQLAEG
                     VPEEDTLDLPARG"
     gene            complement(14026..14709)
                     /locus_tag="VF517_10135"
     CDS             complement(14026..14709)
                     /locus_tag="VF517_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053342.1"
                     /translation="MRIKTITATCALASLGLASAALADHKPGHSPGQGASDVTISATS
                     PIVWGRSTVITGAVRGAGAGVLVDLQSDAFPYADNEFVKDATVPTDAKGNYRFAPLPK
                     LNTRYRVVAATSPPVTSTAATVLVQIRVKVAVSDSTPRRGSLVTFSGTACPQHDGNLV
                     YIQRRTSTGSYGTVARTVLRDAGDTCSTYSRRIRVYSDGVYRVKVSSGGDGDHMTGVS
                     RAVRLDSHR"
     gene            14986..15534
                     /locus_tag="VF517_10140"
     CDS             14986..15534
                     /locus_tag="VF517_10140"
                     /inference="COORDINATES: protein motif:HMM:NF025590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEX8053343.1"
                     /translation="MKRITPLMLLLVALSVAACGGKTLDTSNVEKDIKKIASEGGVAT
                     EVKCPDEVTDVKKGKTYECTITYAGNEKNKQTVEMKIAANDESEFANQKAVQDEGLIR
                     QIVAQSDEDPATVCEHLEESILEQLGGDDCPTKAAEEDDGKPTDIKSIEIQGDSATMV
                     TSESTTTFERDTEGGWIVTAIE"
     gene            15646..17268
                     /locus_tag="VF517_10145"
     CDS             15646..17268
                     /locus_tag="VF517_10145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8053344.1"
                     /translation="MPNHDPPPAERAGQPRPRLLLALAVPGIAASLAAYALLWGQAYR
                     GPTALVLLFVAAIVYATFRESELRMGSISICGNVIPFFAAVELAGPLIAGIALTVAHV
                     VLDVRRRRSFCIVLSNVLSHLLVALAAAGALALLDDLLTTRSGEPLHLLVLIVAGFSA
                     DSVTYLMLVGLTWVETNQPSRDGILRPYLGVARIVALGMCMTAAVVWVHQEGGLAALA
                     VALVALPVFYELVDRINRIENELRVERDRNARYLAVAGSMFLVLDRDGRITLINERGA
                     ELLGRPESELVGADWFALTAPADEVSARREDFVRRLARGDAPPGSESSLALGDGDERI
                     VTWNTTVLRDDDGSASGMLVSSEDVTARRRAEERVAYLAYHDQLTGLGNRVAFEQRLE
                     RTLAGADAAGAAVALYFLDVDGFKAINDELGHAAGDGLLREIGTRLGEAVRGDDLVVR
                     QGGDEFLLLSRMARDGLDTRLENLRARVEEVFERPFDIDGRALAISVSIGVAVYPDHA
                     ADGEALIRRADDEMYRAKRRSGRARIAHAPGA"
BASE COUNT         2484 a         6414 c         6377 g         2187 t
ORIGIN      
        1 gcgcctccgt caggcggtcg agcgcgggct cggccacgac gcccgcgatc accgcgccgc
       61 cgtccgcgcg cacggccgcc acgctcacgg cccagtccgg gcgaccgtag aggtagttgg
      121 tggtcccgtc gatcgggtcg accatccact cgacgccgct ctcgccccgc gtcgagccgc
      181 cctcctcgcc gaccacaccg tcgccggggc ggtggcgcga cagcacggcg cggatcgcgt
      241 cctcggcgtc ccggtcggcc tggctcacga ggtcgtcgga ggcggccttc tccccgaccg
      301 acaggcggtc gaggtcgtgc caccagctca tcgcggccgc cgcgccggcg ccggctgcct
      361 cgcgcgcgac ttcgagcagg tgcgtcaggt caccggccgc ggcggggtgg gtcatcgatg
      421 tgacgattgc agggctccct gtcgcggcgt gcggcggggc gagccggcgg ccgcgcgcgc
      481 ggccaggcgc ccgcgcagcc cccgcccgac cgtggtgagg ctctgaccgc tgaagaacgc
      541 atggctcccc agcaccgcga gccacggaag cgcggccagc agcgggcgct cggtccccgc
      601 gagcgcgagc accgcggcca gcgccgcggc gcccgccgcc acggacgccc agcgccaacc
      661 ggcgggcgcc gcgacggcgc caagagcgag cagcaggccg gcggccggcg ccaggaggtc
      721 gccgctcgag tccacgagca gccggccggt gagcgcggcc gcggcgcccg cgacgagtat
      781 cgcgacgttc cacggcgccg cgcgcggtgt gggctcgctc agtgcggccc aatcgcgcag
      841 gggcaccggc gccctccccg cgggtcccgc cggcacgcgg gcgggcagcc agcgcacgag
      901 cgcgaggagg tgctcgcgcg cctgcccggc gtgcgcccgc cagaccaccc agggcccgtc
      961 gagccgcgcg aacgccagcg cctcgaccgc ccagcgcgcg gtgacgaacg ccacgccgaa
     1021 cgcccacatg gtcaccaccg cggcgaccag cagcgcgggc tcgcccgtga ggtcgaccgc
     1081 gagggcgagc ccgcccacgc cgcggaccgc gtacccgccg ccgaccgcga tccacagcgc
     1141 gacgagggtc ggtcgcagcg gcgggggaac ggcatcggtc cggccggtgg ccgccgcgcg
     1201 cagcgactcg tacacgcccg ccaccgcgaa taccgccacg ccgagcacca gcagcacgcc
     1261 gccggcatcg aggccggaga tgccgacggc gacggccgcc gcgatcgcga gccgtgccac
     1321 gccggcgagg gcactggccg cgatgtgcgg ccgcgcgcgc tcgaccggcc cggggaggcg
     1381 cccgcggctc gccgcgtcgg gatgagcctg gtcggcggcg aagccgcgga tgtcgttcca
     1441 ctggtagcgc gcctggtaga tcagcagctc gaggcacgcc cagacgaccg ccgcgcgcag
     1501 caactcgtgc gccgacgcgc cgccatcgca gagcacgccg aggccgaagg cggccgggag
     1561 gatccacgcc ttgccgaggt ccttcggacg cggcaggagc aggtagctcg gcagcgaccg
     1621 gccgggggcc gagtcgctca cgcggacgcc ccggctgcgt cgcggcgctc gctcagccgc
     1681 gggcgcggca tgagcagccg ctcgagctcg acccacgagg acacaaccgc cgccgctccg
     1741 gcctcggcca gcgccgcccg gcgcgccgcg cgctcgtcgc cggggacgaa catgacgttc
     1801 ccgaccgtgg ggatcccggc ggcgagcgcg gagcgggcgc cgggaaccga gtcctcgacc
     1861 gcgagcgcgc gccgcgcggg cacgccgagc tccgccagcg cgtgcgcgta gatcgcagga
     1921 tccggcttgc tcctggggac cggcagcgag tcctcggcgc tgaagcgccg cccgggcggc
     1981 agcagctccg ccagcccggt cgcctcgaag caggcggcga gacgcgcgag cgcgctcgag
     2041 ctgaccgccg cgagctcgag gtcgcgtgcc agccgttcga gcgggcgggt cacggaccgg
     2101 ttcggcttca gcacgcgtgc gagatgagcg gtcaccgcgc gggcctcctc ctcgacccag
     2161 tgctcgagct cgtgggacgg gagtggccgc cccgcggcga ccgccaggtc cccagccgtg
     2221 ctgcggaagt tcagcccagt ggtcctgagg cgcaggtcga ccgggtcgaa ccgcaccgtg
     2281 gagccgatcg cctccatcat ccggttcgtc accacggccg acgcctcgaa cgccggctcc
     2341 tcggatggga acaggttgcc gtccgcgtcg cacagcagca cctgcacggc cgccggctcg
     2401 agcgggaggt tgcccatcag gccgccctgg ccgctcgcgc gtcgaggtgt ccggcgacgg
     2461 cggcgtgcac gccgtcgcgg tcgagggtca tgaggtcgcg ccggaccgcc gccacgaagc
     2521 tcgcgttctg gcccaggtcg ccgaagaggt cgcggcgggc gagcagcggc cgggggtcgc
     2581 tcccgcccgc ccgtgcgagc ggctggagct ggtccaccag cgcgtccttg acctcgatgc
     2641 gctgcccggc gcagtcgctg ccgcgcaggt agcgcagcca ggcggcgacc gcgagcgtga
     2701 gcagcgggtg cgaccgtccg cgcttctgcg agtcggcgat cgagggcagc aggtaggccg
     2761 gcatcttcgt ggatccgcgg ccgcagagcc gcgcgagcgg gtcgccgatc atcgggttcg
     2821 cgaagcgttg cagcagggtg ctctggtact cgtcgcgatc gacgccgggg gtggccggca
     2881 gcagcggcgc cacctccctg gccatgaggt cggtcacgta tgcctcgagc agggggtcgg
     2941 ccatggcgtc gtcggtccgg acgtgccccg cgagcgagcc gaggtagccc agggccgagt
     3001 gcgaggcgtt caggagccgc gtcttcatca actggtacgg ggccacgtcg ctcacgaact
     3061 gcacgccggc ctcctcgagc ggcgggcgca cgtcgcagaa ctcgtcctcg aggatccact
     3121 gccggaacgg ctccgtcacc accggccagc ggtcgtcgac cccgaactcg cggcggatcg
     3181 cggcgcggtc ggcggcggtc gtctccggag tgatgcggtc gaccatgctg ctcgggcacg
     3241 cgacccgggt ctcgatccag cgccccagca gctcgtcgcg catcgtgccg aagcccacca
     3301 gcgccgcacg ggtcacgtcg ccgttcttgg ggatgttgtc gcaggacacg accgagaagg
     3361 gtggaactcc cgccgcgcgc cgccgcgcga gcgcctcgac gatgtagccg atcggagtcg
     3421 tcggccgcga ggggttggcc aggtcggcgc ggatggcctc gccgtcggcg tcgagctcgc
     3481 cggtggcggc gtccacgtgg tagccgtcgc cggtcacggt gagcatcacc acgcgagtcc
     3541 ggctgtccgt gagcgcgccg aacaccgcct cggggtccgc cggtccgaag aggtagcgcg
     3601 gcatggagcc gacgacgcgc gcgctgctgg cgccctcgtc gcgctccacg acggtgtaga
     3661 ggcagtcctg gggcgcgagc gcgtcctgca tgtgcggccg gcgcatgccc acgccgacga
     3721 tgccccagtc catcgtcacg ccgtcctgtg cgaggtcgtc gaagtaggtc gcctggtgcg
     3781 cgcggtgaaa gccgccgacg ccgaggtgca cgaccccggg cctgagcgcg cggcggtcgt
     3841 aggtcggcac gcggacacgg gcgccgtggg cgtggagcgt gcttgcggcc agcggtcggg
     3901 cattcggcac ggctcagtcc cctctcggtt cggttcctcg tactggaggt ccgccgggca
     3961 gtcctgagga gctgcgtcgt cccgtcctgt ctcatgtccc cggtcgcccc gtccggcgag
     4021 gtcgaggggg gcgatcggaa cgttgcccgc gcgcggcccg ccccaaacag ggccaccgcc
     4081 accggatgtc tcaggcgggc ctccgggcgg cccgcgcgct caggggtaga agcgcgcctt
     4141 cgcggccttc gcgaccggcc gcggcagctt gccaagcacg ttcagcgcgt gcggcatcgc
     4201 gcgcagcctc agcccgctgt acacgctcgg gaccacggcc tcgacgagat gcggaccggg
     4261 ctccgcgacg gcctgctcca gagcggtcac gagctcctcg ccggtctccg ttcgcacggc
     4321 cggcacaccg aggccgtttg cgatccgcac gaagtcgagg tccggcccgg cgaggtcgag
     4381 ctgagacttc gcccgcggtc ccggccgctc cgccccgacc cgctcgagct cgatgttgag
     4441 gatcgcgtag gagcggttgt tgaagatcac cacggtcacg tcgagctcct cgcgcgccat
     4501 cgtccagagg gactggatcg tgtacatggc ggacccctcg ccctccagcg cgaacaccgg
     4561 acggccgggg cacgcgatcg ccgcgccgag tgcgaccggg aggccctgcc cgatcgctcc
     4621 gccggtcagc gccagcaggt cgtggggcgg cgcgccggcc gtgtgggtgg cgagcgtgac
     4681 ccccgaggtc tgcgactcgt cggagacgat cgctccctcc ggaagcaccg cgccgatcgc
     4741 ctggcagacc ttgtccgcgt cgagctgccc ggtcgggcgc gccggccgcg acgccgcctg
     4801 gcgcaccggc tcggcggcgc cggccccgag cgcgtcgacg agcgcctcga ggccgcccag
     4861 cgcgtcctgg tcggatgccg cgagctcgtg cacctcgcac ccgtccggaa ccaggtagct
     4921 cttcttcccg gggtaggcga agaaggacac gggcgccttc gcgtccacga ggatcaggtg
     4981 cttcaggccg gcgagctgga ccgacgccag ctcggccagg tacgccacgc gctcgatcgg
     5041 ggggatgccc gcgccgcgct gcaagcgggt cgggaagacc tcggcgagca gcttggcgcc
     5101 cgtggccgcg gccacccgtc cggccgcgac gagcgcgggc tcgcgcagcg cccggccgcc
     5161 cagcaggatc gcggtgtcct cgccgccgcg cagcgcgctc accgcctcct cgaccacctc
     5221 ggcgggcgcc acgggcggag cggcgggggc cgccgccggg gcgggctcgc cgccctccga
     5281 ccacgacacg tcggcgggca ggatcagcgt ggccacctgc cccggcgggc ccttggccac
     5341 cgcgatcgcc tcggcggcgt cgcgccccag ctcggccgtg ctccgcgacg tccggaccca
     5401 gctcgacacg ttgcgcgcca ctgtctcgat gtccgactgg agctgcgcgt cgtactgcgt
     5461 gtggtgggtc gcgtggtcgc cgacgatgtt gacgatcggc accctccccg tgcgcgcgtt
     5521 gtggaggttc gccaggccgt tgccgaggcc ggagccgagg tgcagcagcg tggccgcggg
     5581 agtgcccgcc atgcgtgagt agccgtcggc ggcgccggtc gcgacaccct cgaacagcgc
     5641 cagcacggcc cgcatctcgg gcaccgagtc gagcgccgcg acgaagtgca tctcggaggt
     5701 gcccgggttg gtgaagcacg tggtcacgcc gccgtcgacc agagtcccca ggagtgcccg
     5761 cgcgccgttg gtcacgccgg gagtgtcgca ccgccctcga cgacgtgccg cgcgggttgt
     5821 tcgcgctccg tccggcggaa tcgtgagcat cgacggatgc cgaccacgac ccctctgcct
     5881 ccccgcctag actggcgccg tgagcctcca agagctgcgt agccccagcg cgaccgacca
     5941 gccggaaccg ggcgaccagc cgctgggcgt gctgcccgcg cagggtctgc gcggcgccat
     6001 cgacaagggc tggatctccg gcggcgatta cgcgatccgg ccggccagcg tccagcccgc
     6061 cagcctcgac ctgcgcctgg gcgaccgcgc ctggccgctg cggtgcagct tcctcccgga
     6121 tcccaagtcc acggtcatgt cgcggctcca cgacctggct ctggaggaga tcgacatcag
     6181 caagggcgcg accctggagc gcgaccgtgc ctacctgatc ccgctggtag aggagctcgc
     6241 gcttcccggc gaggtccgcg cgaaggccaa cccgaagagc tcgaccggcc gcctggacgt
     6301 gttcacgcgg gtgatcaccg acggcaacca ccgcttcgac gagatcccga agggctacag
     6361 gggcaagctg ttcctggagg tcgtgccgag gtcgttcgcg gtcaaggtcg agaagctgct
     6421 gagcctcaac cagctccggt tggcggtcgg cgatccgcgg gtgcgcgacg acgacctgcg
     6481 aagggcccac caggagttcc cgctgctcta cgtgggcgag cgcaaggtgc ccgacgagga
     6541 cctggcggtc tcggacgggc tgttcctcag cgtcgatctc agcggtccgc ccgacaggat
     6601 cgtcggctac cgcgcgaaga agaacagcca ccggatcgac ctgtcgcaga tccggcacta
     6661 ccgctggcgc gactactggg agccggtcta ccccgagcgc gggggccgga tcgtgctgga
     6721 gccggagatc ttctacctgc tgctgtcctc cgagcgcgtc tgcgtgccgc ccgagtacgc
     6781 cgccgagatg atggcctacg accccaccgc cggcgagctg cgcacgcact acgcggggtt
     6841 cttcgacccc ggcttcggct acgaggacac cggcgggaag ttcggttcgc gcgccgcgct
     6901 cgaggtgcgg gcgcgcgacg tggcgttcat ggtcgagcac ggccagccca tctgcaagct
     6961 cgcgttcgag cgcatgctgg agccgccgga cgagcactac ggctccgcgg tcggctcgaa
     7021 ctaccagggc caggagacga tgctgagcaa gcacttcgtc gagcagggcc tcgggcgcgc
     7081 gcaggccggc gccggtgccg cggaggtcga cgccgccacc aacccgctgt tcgagtagcc
     7141 gcatggcgct ctggctcgtc atcgccgtcg ccgtgctgct cgtggtcggt gtcgccctga
     7201 tctacaacag cctcgtcgcc aagcgcaacc gagcggagaa cgcctgggcg caggtcgacg
     7261 tgcagctgcg ccgccgccac gacctgatcc ccaacctggt cgagacggtc aagggctacg
     7321 cggcgcacga gaagggcacg ttcgaggcgg tcaccgagac gcgcgccgcc gcgcagcagg
     7381 cgcaggggcc ggccgcgcag gcgcaggcgg agactgctct gtcgggtgcg ctcggccgcc
     7441 tgatggccgt cgcggagaac taccccgcgc tgcgcgccac cgagaacttc cagcagctcc
     7501 agggcgagct gggcagcacc gagaacagga tcgcggtagc ccggcaggtc tacaacgaca
     7561 ccgtcctgac cctcaacaac gcctgccagc aggtgccgac gaacatcgtc gcgaacgcgt
     7621 tcgggttccg gctgcgcgag tacttcacgg tcgacgcggg cgatcccgcg cgcgatccgg
     7681 tctcggtggg attctgatgc ggcgggcgcc ggccgcgctg gcggcggcgc tcctcgtcct
     7741 cctgctcgcc gccgccccgg ccggggcgaa gtcgttcgcc ctctcggacg ccgacgtgga
     7801 cgtgcgcgtc gcacccgacg gcgggatcgt ggtgcgcgag tcgatcacct tcgactacgc
     7861 cggctcgttc agcggcgcct tccgcgacat cccgctgcgc aagggcgagt ccatcgaccg
     7921 ggtctcggtc gcggagcgcg gcgagcgcta caagccgggc gggtcgctca cgctcggcac
     7981 cggcggcccg gcgtcccagt tcggctccgg cgtggtcgac ggcaagcagc ggattgtctg
     8041 gcgcttccgg gccctcgacg agcggcggac gtacacgatc cgctaccgga tctcgggcct
     8101 cgcgctggcc tacgacgacg tggtcgacgt gaacctccag gtctggggga gcacatggga
     8161 gaccgggctg gcgcggctgc gggcgacgat ggccgcgccg cgggaggtga ccgcgcgcaa
     8221 cgcgcgcgtc ttcggccacc cggtcacggt gcccggggac accgccttcg gcggcggcgc
     8281 ggcccacctg cgcgcgctcg acgtgcccaa gcagcagttc gtggagatgc ggctgctgtt
     8341 cccgcgctcg atgctgacct ccaccaagcg cgcgaaggtg gcccgcggtg aggcgcgcac
     8401 gcggatcctc gccgccgagc gcgccgacgc ggccgactac gagcacaacc gccgcaagat
     8461 cgacgacgcc gtcgatcacc tcccggccaa cctgctgctc ctgctgttcc tgggcgtcgc
     8521 cccggccgcc gcgatcctcg cgctcgtgtg gctcgtgctc tcccgcgagc gccgcgccgc
     8581 ggactacgac cgtgagtacg agcaggagcc gccgtccgac gacccgccgg cgctgatcgc
     8641 gccgctgctg cgtcagtcca cgaagcccgg cggcgaggag ttcaccgcca cgctgttcga
     8701 cctgatccgg cgcggctacc tgaccaccga gccgaccacc accgagaagt ggagctgggg
     8761 cgcgctcggc aagcgcgagg ggcccgacct gctgctctcg cgcaagggcg ccaccggcga
     8821 cgccgcgccg ctcgaaaagg gcgtagcgaa gatcctcgag cgggcgctcg gcaacgggcc
     8881 gctcccgctc tccgagctgt ccgagaagat caagggcaac cgcgaggcga acgccaacag
     8941 ctacagctcg tggcaggaga agatcggcaa ggagttcggc cgccgccact ggtacgacga
     9001 ccgcgccgcg aaggtgatcg gtgccgcggc cgctgcgatg gggctcgccg cgatcgcgct
     9061 gatcgcgatc ggcgtcgtag ggatcgactc gcgcttcgtt cgctggggcg aggtcgtgca
     9121 gatcgcgctg ggggtggcgg cggcgatcaa cgcggtcgtg ctcgtcgtct cgctgcgcaa
     9181 cgagcggctg cgccggcgct ggacccccgc ggccgcgctc gaggcggcgc gctgggccgc
     9241 gttccgccgc tacctgcgcg acttcccccg gctccaggag gcgccgccgg agtcggtcgc
     9301 gctgtgggag cgcctgctgg tctacggcat cgccttcggc ctggccgatc gcgtactcca
     9361 ggcggcgaag atcgccgcgc cggaggcgct ggcgcagagc ggcatctacg cacccggcta
     9421 ctacgccggc ggcgagacct tctactcggg ctcgagcatc tccagcgtgt cgagcggctt
     9481 ctcgagcgcc ctggcgccgc cgtcgagcgg gtccgacggc ggtgggggcg gcttttcggg
     9541 cgggggtgga ggcgggggcg gaggaggagg cggcggcgcc tggtgagccc gctccgcgag
     9601 atcaacggcg ttcggctgct ggtcgaggag tcgggtagtg ggcccgcgct cgtcctcgtg
     9661 cacgggagct gggacgaccg gcgctcgtgg gggcggatcg agaacgagct gagtcgccgc
     9721 taccgggtgg tgagctacga ccgccgcggc cacacgggca gcgatgacgg ctacccggcg
     9781 gggacgcgcc ggaccgacga ggacgatctc gcggagatca tcgaggcgct ggggctcgca
     9841 cccgcgcacg tggtcgcgag ctcctttggc ggctcgatct cgctcggcct ggccgcgcgc
     9901 aggccggagc tcgtgcagac cgtctgcgcg cacgagccgc cgctgctgtc aatcgcggcc
     9961 gacgaccccg aggtcgcgcg cgtgttcgag accatgcgct cgctcctcga gctgatcgat
    10021 cgcggcgagc gcgaggccgc cgcgcgcggc ttcgcggacg acatcgccct cgggcgcggc
    10081 agctgggcga tgatcccgac cgacgtgcgt gcgtcgatgg tggcgaacgc cgagacggcc
    10141 gccggtgagg tccgcgaccc cgagtgggcg cagctcgacc tggacgggct gagccgcgcc
    10201 ggggtgccgc tcctcgtcac tcagggcaat acgagcccgc tgttcttcag gcggatcatc
    10261 gagcggctcg cggcggcggt cgagggcgcg gaggtcagga cgctgccggg cgccggccac
    10321 ctcccgcacc tcacccaccc ggcggagtac gtggccgccg tcaggggctt catcgagcgc
    10381 tcggcttgag accgcggccc tcgtagagcg ccgagcggtc gagcccggcc accgagttct
    10441 gaccggtgag cccgagcgcg gtgtcgatgt cggcccgcat gccctcgatc acgtgtcgca
    10501 cgccggcctg gccgcgggcc gccactgccc acgcccaggg ccggccgatg agcacggcgc
    10561 gcgcccccag cgcgaccatc ttcaccacgt cgaggcccgt gcgcaccccg ccgtcgacga
    10621 gcacctcgac ctcgtcgccg atcgcgtcca ccacgtcggg cagcgcgcgt gccgtggagg
    10681 gcaccgagtc gagctggcgc ccgccgtggt tcgacaccac gaccccgtcc acgcccgcgt
    10741 cggcggcccg gcgcgcgtcc tcggggtcga gcacgccctt cacgaccagg cggccgttcc
    10801 agtgctcgcg cacccaggcg atgtcgtccc aggtcacgct cgggtcgaac tgctcgtcga
    10861 cccactgctt gaagtcggcc ggtgtgctgg cgtccggcac ggcattctcg aggttgccga
    10921 acgtgagcgg cttgccgccg accgccacct cccgcagcca gcggggatgc gagacgaggt
    10981 cgaggccgcg ccgcgccttc gcccacgcgg tgtgctcgcc gaccatcgcg ttgcgcgtgt
    11041 cgcggtggcg cgcaccgacc acggcgaggt cgatcgtcag catcaggacc ggcgagccga
    11101 ccgcctgagc gcgcgccatt aggtcctgcg cgtagccgcg gtcgcgcatc acgtagagct
    11161 ggaaccacgg cggcgcggtc gtcgccccgg ccacctcctc gatgctgcag atcgagacgg
    11221 tcgactcgac gaacggcacg cccgcggcct cggccgcgcg cgcggccagc acctcggcgc
    11281 gcggcgcgca catcccggcg agccccaccg gggccaggac gatcggcagc gcgagctgct
    11341 gaccgagcac ctcgacggcc tgctcgcgga cgctcacgtc gcgcatgacc acctgccgca
    11401 gcaggacccg gccgaggtcg gacacgttcg cgcgcatcgt ggactcgtcg taggcgcccc
    11461 cgtcgacgta gtcgaagagc tgccgcggca gccgccgccg cgccagctcg cggtagtcgg
    11521 cggcggtcgc gggggcgacg ccgagcgggc gatcggctct gtagcgggtc atgccgcgga
    11581 tgttcccaaa acggcggctg gctgagcagc cgcacccgcc ggccgcacga gcgcaggagc
    11641 gccaagccgc cgccggcggc cctcgccgtc ttgacagcac ggcgcttcga tcagagggtg
    11701 gatccgctcg caacggaagg gattcggatg atcaaggcag gtacgcgccg cgcgatcgcg
    11761 gcgtcgatcg cagtcgtggc cgcggccgcc gtgccggcgc cggcgctggc cgccgaggcg
    11821 cacgtcgaga cgatcgacgg cgtgaaggtg atcgtgttca acgccgctcc gggcgagctc
    11881 cagagcgtgc tcgtggaccg cgaccccggc ttcacgaacg accagtacca gctcgaggac
    11941 cagaacaaca aggtgagcgc cggcacgggc tgcgcgaacc cgccgcgcac gccgttcgcc
    12001 ggcgtcgagg agaagatcgt ccgctgcgac gcgagcgcca cgacgatccg cgtgaacctc
    12061 gccgacgggc gcgacagcgt gcgcttcggc gacctcagcg gccacctgat caccgacgtg
    12121 gtcgacggcg gcgacggcga cgacagcctc gacacctcca acggcagcgg cctgatcatg
    12181 ggcggcccgg gcgacgacac catcgacgcc aacgccggcg acgacgaggt catcggcggc
    12241 gacgacgacg acacgatcgt gggcggcccc ggctcggaca ccatcagcgg cgacgccggg
    12301 gacgacaaga tccgcggcca cgcgacgccc gacacgcacg agatcggcaa ggtgaccgac
    12361 ttccgcgccg gcgacgtgaa caaactcgcc ggcgggcccg gcaacgacac gatcgtcggc
    12421 gacaacggga ccgacgagct gacgggcgac acgggcaacg acaacctcaa cggcgccggc
    12481 gcggcggacc tgctcgacgg cggggccggc aacgacacca tcgacgaggg cgacacgccc
    12541 ggcgacccca acggtggcgg cccggcgggc ccgctcgcgc cggacacggt ccgcggcggc
    12601 tcggggaccg acaccgccac ctactgcacg cgccactact cggactccga ggcgcgcaag
    12661 cacacgctca cgatcacgct cgacgacaag gccaacgacg gcgagaaggg cgagaaggac
    12721 cagatcggct cgaagggcga cgtcgagaac gtgctcggcg gcggcgtgac gaacgacagg
    12781 atcaccggca actctcgggc caacgagctg atcggcgact gcctgagcac gttcacctca
    12841 tcgggcgaca acaagctcta cggcgcaggc ggcaacgaca agctcgtcgg ctcgagcggt
    12901 cgcgacctgc tgaacggcgg caagggagcg gaccgcttcc tcggcaacga ggacgccgac
    12961 acgatccagg ccaaggacgg cgcgcgcgac aagtcgatca actgcgacgg gctcggggcg
    13021 aagtcgagca aggacagcgc gacggtggac cgctcggacc cgaccccgtt caactgcgac
    13081 aagctgcgcc gctgaacggg gagctagccg cgcgcgggga ggtccagcgt gtcctcctct
    13141 ggcacgccct cggctagctg cttctcgcgc tcgatctcgg cgttgacctc ggcgccgaac
    13201 aggagcgcga cgttggtgag ccagagccag aacagcagga tgatcgcggc ggcgaacgag
    13261 ccgtaggtga cgttgatgga gctgaagtgc ccgatgtact cggagaacgc cgccgaggcc
    13321 gcgatccaga cggccacccc ggcggccgcc cccggcgtga gccaccggaa cgcgcgctgc
    13381 ttgacgtcgg gcgtcacgta gtagaggaac gagaagacga gcatcgcgct cgccagcgcg
    13441 agcggccagc gcaccaccat ccagaccgac gccgccgtcg agcccgcgag gtcctccgcg
    13501 gcctccgcgc cgacgaagac cagcacgagc gtggcgagca ccagggcgaa gagcacgacc
    13561 gtggacatca cgtcggtcag cttgcggcgc aggaagctcc ggccgtcgcc ggcctcgaac
    13621 accacgttca tcgcccggcg cgccgcctcg aggtagcccg tggtcccgta caggacggtg
    13681 agcgtgctga tgagcagcgc cagggcggcg ctccccttgc tccggagggc cgcgcgcacc
    13741 gcgtcgtcga tcggcgcgag cgtggtggcc ggcacgacgt cgcgcaggta gtcgacgatc
    13801 gagtcgtagg tgccggggta ctcgccgagc aggcccagca gcgacacgcc cagcagcagc
    13861 gtggggaaca gcgacatcag cgcgtagtac gtcagcgccg ccgcgtggtg ggtggcctgg
    13921 tcgtcgtaga acgaaaagag gacccgcttg atgaccgccc acgcgtcccg cacgcggtca
    13981 acgtaccgcc ggggagccaa acggcccccc ggcgcccgtc cctggttatc ggtgcgagtc
    14041 gagccggacc gcgcggctca cgcccgtcat gtggtcgccg tcgccgccgg agctgacctt
    14101 cacccggtac acgccgtcgc tgtagacgcg gatgcgccgc gagtacgtcg agcaggtgtc
    14161 acccgcgtcg cgcagcaccg tgcgcgcgac cgtcccgtag ctgcccgtgc tcgtccgcct
    14221 ctggatgtag acgaggttgc cgtcgtgctg cgggcacgcg gtccccgaga aggtgacgag
    14281 gctgccgcgg cgcggcgtcg agtcgctcac cgcgaccttc acgcggatct gcacgagcac
    14341 cgtcgcggcg gtgctggtga ccggcgggct cgtcgccgcg acgacccggt agcgcgtgtt
    14401 cagcttcggc agcggcgcga accggtagtt gcccttggcg tccgtgggca cggtggcgtc
    14461 cttgacgaac tcgttgtcgg cgtaggggaa ggcgtcgctc tggagatcga ccaagacgcc
    14521 ggcgccggcg ccgcgcaccg ctccggtgat gacggtcgag cgaccccaga cgatcgggct
    14581 ggtggcgctg atcgtcacgt cgctcgctcc ctggccgggg ctgtggccgg gcttgtggtc
    14641 ggccagcgcg gcggatgcga gccccaggct cgcgagcgcg caggtcgcgg tgatggtctt
    14701 gatcctcatg ggtcccactt tcagattggt cctcgcctcg tatccccata acgggcacga
    14761 gggccgaaag ttgggtatac cgcggccgcg ccggccccgt ctgacggcgg gttggcgcca
    14821 gatgccccgg gcgggcgccg cccccgcgtt aggttcggag gctgtgagcc tcctctccgc
    14881 cgactcggaa tcccgtcgat tccccgtttc acaggcggaa atcggtggcc ggtcgcgaat
    14941 cccatcgcga cgaccacttc actgaaacca cgaaagagag aacgcatgaa gcgcatcacc
    15001 ccgctaatgc tgctcctcgt cgccctctcg gtggcggcgt gcggcggcaa gaccctcgac
    15061 acctcgaacg tcgagaagga catcaagaag atcgcctcgg agggcggcgt cgcgaccgag
    15121 gtcaagtgcc cggacgaggt cacggacgtc aagaagggca agacctacga gtgcacgatc
    15181 acctacgccg gcaacgagaa gaacaagcag accgtcgaga tgaagatcgc cgccaacgac
    15241 gagagcgagt tcgccaacca gaaggccgtg caggacgagg gcctcatccg tcagatcgtg
    15301 gcgcagagcg acgaggatcc ggcgacggtg tgcgagcacc tcgaggagtc gatactcgag
    15361 cagctcggcg gcgacgactg cccgacgaag gccgcggagg aggacgacgg caagccgacg
    15421 gatatcaaga gcatcgagat ccagggcgac agcgccacga tggtgacctc cgagtccacc
    15481 accacgttcg agcgcgacac cgagggcggc tggatcgtca ccgcgatcga gtagagcgac
    15541 gtagccgcgt tccggggtcg ggccaggcgc cgggccccgg gccgccggca atcacccgcc
    15601 tggggggtag ggacgagcgc caacgcgccg ataacggtcc cagcgatgcc gaaccacgac
    15661 ccgccccccg cggagcgcgc cgggcagccg cggccgcggc tgctgctggc cctcgctgtc
    15721 ccggggatcg ccgcgtcgct ggccgcgtac gcgctcctgt gggggcaggc gtaccgcggc
    15781 ccgaccgcgc tggtgctgct gttcgtcgcc gcgatcgtct acgcgacctt ccgcgagagc
    15841 gagctgcgga tgggcagcat ctcgatctgc ggcaacgtca tcccgttctt cgccgccgtc
    15901 gagctggcgg ggccgctgat cgcggggatc gcgctcaccg tcgcgcacgt cgtcctggac
    15961 gtccgccggc gccgcagctt ctgcatcgtc ctctcgaacg tcctctccca cctgctggtg
    16021 gcgctcgcgg ccgccggcgc gctcgccctg ctggacgacc tgctcacgac gcggtccggc
    16081 gagccgctgc acctgctggt gctgatcgtc gccgggttca gcgcggacag cgtgacctac
    16141 ctgatgctcg tcgggctcac ctgggtcgag acgaaccagc cgtcgcgtga cgggatcctg
    16201 cggccgtacc tcggcgtggc tcgcatcgtc gcgctcggga tgtgcatgac cgccgccgtg
    16261 gtgtgggtcc accaggaggg cggcctggcg gcgctcgcgg tggcgctcgt cgcgctgccg
    16321 gtcttctacg agctggtgga ccggatcaac cggatcgaga acgagctgcg cgtggagcgc
    16381 gaccgcaacg cgcgctatct cgcggtggcg ggctcgatgt tcctggtgct cgaccgcgac
    16441 ggccggatca cgctgatcaa cgagcgcggc gccgagctgc tcgggcggcc ggagtccgag
    16501 ctcgtcggcg ccgactggtt cgcgctcacc gcgcccgcgg acgaggtgag cgctcggcgc
    16561 gaggacttcg tgcggcggct cgcgcgcggc gacgctccgc ccggcagcga gtcgagcctc
    16621 gcgctcggcg acggcgacga gcgcatcgtc acctggaaca ccacggtcct ccgcgacgac
    16681 gacggcagcg ccagcggcat gctcgtctcg agcgaggacg tgaccgcacg ccggcgggcc
    16741 gaggagcgcg tggcctacct cgcctaccac gaccagctca ccgggctcgg caaccgagtc
    16801 gcgttcgagc agcgcctcga gcgcaccctg gcgggcgccg acgccgccgg tgcggcggtg
    16861 gcgctctact tcctcgacgt cgacggcttc aaggcgatca acgacgagct cggccacgcc
    16921 gccggcgacg gcctcctgcg tgagatcggt acccggctcg gcgaggcggt gcgcggcgac
    16981 gacctggtgg tccgccaggg cggcgacgag ttcctgctgc tcagccggat ggcccgcgac
    17041 gggctcgaca cgcggctgga gaacctgcgt gcacgcgtcg aggaggtctt cgagcggccg
    17101 ttcgacatcg acggccgcgc gctcgcgatc tcggtgagca tcggcgtggc cgtctatccc
    17161 gaccacgcgg ccgacggcga ggcgctgatc cgccgcgccg acgacgagat gtaccgcgcc
    17221 aagcgccgct ccgggcgcgc gcgcatcgcg cacgcgccgg gcgcctgacg cgcgcgtcgt
    17281 agcgggcgcg gggccgcgac ctcaggcgcg ggctggcacg gcgccgctca ggcggaaccg
    17341 gacgccgccg atctcgaccg actcgcgccc gcggcgcagc tcgtcgggca gcgcgaccgc
    17401 gacctcgacg agtcgctcgg cggactcgct cgccgcgcgc tcgaaggcca cccatccgcc
    17461 gt
//
LOCUS       DASVPL010000195         4588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_179321, whole genome shotgun sequence.
ACCESSION   DASVPL010000195 DASVPL010000000
VERSION     DASVPL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179321"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VF517_10150"
     CDS             <1..627
                     /locus_tag="VF517_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEX8053345.1"
                     /translation="IHVATLEKRTKLEIYLTVGHGRGYSPAEDNKTPDQPIGVIPIDS
                     IFSPVKRVAYNVEAARVGQRTDYDKLTLDIETDGSVAPQAALREAAEILIKSLAIFTD
                     ADRLEALQSAGLGGGGADGYGEVAAAAGPAAVGSGRSPDGMDDILIEELELGVRSYNC
                     LKRAGIQTVGDLLQKSESELNAIPNFGRKSIEEVIETLKSRGLDLRPG"
     gene            658..1011
                     /gene="rplQ"
                     /locus_tag="VF517_10155"
     CDS             658..1011
                     /gene="rplQ"
                     /locus_tag="VF517_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX8053346.1"
                     /translation="MRHQRTRNKLSRSASHRKALFMNLSREVFNHERIKTTEAKAKAV
                     APEVEKLITLAKRGDLHARRQALSALGQDKFVVYKLFEEIAPRYAERNGGYTRILKLG
                     PRHSDATEMVYLELV"
     gene            1040..1825
                     /locus_tag="VF517_10160"
     CDS             1040..1825
                     /locus_tag="VF517_10160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8053347.1"
                     /translation="MKAESRESWGVNPYAPLADLLRPAAVELVRACGIGPGMRVLDVA
                     AGNGNAAVEAVRAGAEAVASDLAPLQVEQGRERTRAEGLDIEWVEADAEELPFEDGSF
                     DASMSVFGAMFAPRVDRAAAELFRVVRPGGTVGMANWRPDGFQARFFDVLNRYRDPDP
                     PGVEPASLWGDEEHVRERFGPHADEVRVEPRMLPWQWSSLQEMGEFFAATRPGGSAAG
                     MSEERRQQLAIDVLELVQEHNTAEGGAVYIDSAYSIVVARRKS"
     gene            1840..2556
                     /gene="truA"
                     /locus_tag="VF517_10165"
     CDS             1840..2556
                     /gene="truA"
                     /locus_tag="VF517_10165"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX8053348.1"
                     /translation="MEYDGSGFSGWARQPGHRTVQGVLEEALERVRPEPAALTVAGRT
                     DTGVHALGQVASHPGEPALARSLNGVLQRDVRVVASERAPDGFDARRDALSRTYRYRV
                     HTREAQSPFERLRSLHWPQALDRDALDRCAALLPGTHDFTAFTPTQTDHVRFERDVLR
                     AEWSVEGPDMVAFWIEADSFMRHMVRILVGSMLAVARGYRPLDWFEGLLASAPRSEAG
                     DTAPAHGLYLEAVTYPQDGS"
     gene            2553..3362
                     /gene="surE"
                     /locus_tag="VF517_10170"
     CDS             2553..3362
                     /gene="surE"
                     /locus_tag="VF517_10170"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX8053349.1"
                     /translation="MKVLLTNDDGIQATGLNALRRALLEVPGVELSVIAPNANRSATA
                     RSITTRSPLWVEEIEFEDGTTGFATDGTPVDCVRFSVLGLVDRPELIVSGINHGTNLG
                     DDITSSGTVAAALEGVVLGLPAIAVSQQSEGGEMDFRAGLRFDFEEGAAFVARLVEEL
                     ESVPVPEGTLLNVNVPAGDAKGARVCGLGRRIYRDKLELVEEEDGRKRYRIYGERPGH
                     HEEEGTDFAALSEGLIAVTPLHFDLTDRAGIESLSGFDLDGLIRPAAQEVE"
     gene            3355..>4588
                     /gene="ligA"
                     /locus_tag="VF517_10175"
     CDS             3355..>4588
                     /gene="ligA"
                     /locus_tag="VF517_10175"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX8053350.1"
                     /translation="MSSAEARAAELRRVIEQHNHSYYVLDEPTVGDDEYDSLLNELRQ
                     LEAEHPELRTPDSPTQRVGAKPLDKFEPVRHLQPMLSLANARNEEELRAWDARVRKLA
                     GEEAPEITYGTEPKIDGLAISLVYENGLFVRGATRGDGEIGEDVTQNLRTIKEIPLRI
                     PDAPPLVEVRGEVYLPRSAFARLNEERAAAGEPTFANPRNSAAGSIRQLDPALAASRP
                     LSMWCYGIGTTEGLSFDRHSEALQWMRDRGFKVAPDTKVHADLESLVAECRRWENDRE
                     SLDYEIDGVVVKVDELDLQRSLGVVGREPRGAIAWKFAPMTATTTLRAVMWNVGRTGH
                     MVPFANLEPVQVSGVTVKLATLHNEEDLRRKDVRDGDEVIVMRAGDVIPQVVSPTAKA
                     QRSKKRSAPPEPPAECPAC"
BASE COUNT          692 a         1605 c         1682 g          609 t
ORIGIN      
        1 atccacgtcg ccacgctcga gaagcgcaca aagctcgaga tctacctgac cgtcggccac
       61 ggccgcgggt actcgccggc cgaggacaac aagacgcccg accagccgat cggcgtgatt
      121 ccgatcgact cgatcttctc gccggtcaag cgcgtggcgt acaacgtcga ggccgctcgt
      181 gtcgggcagc gcaccgacta cgacaagctg acgctcgaca tcgagaccga cggctccgtg
      241 gcgccgcagg cggcgctgcg cgaggccgcc gagatcctca tcaagagcct ggcgatcttc
      301 accgacgccg accggctcga ggcgctccag tctgcgggcc tcggcggcgg cggcgccgac
      361 ggctacggcg aggtggccgc ggccgccggc ccggcggcgg tcggctcggg tcgctccccc
      421 gacgggatgg acgacatcct gatcgaggag ctggagctcg gcgtgcgctc gtacaactgc
      481 ctgaagcgcg ccggcatcca gacggtcggc gacctgctcc agaagtcgga gtcggagctg
      541 aacgcgatcc cgaacttcgg tcgcaagtcg atcgaggagg tcatcgagac gttgaagtcc
      601 cggggcctcg acctgcgacc gggctagccg gccgcagcgg ctagggttct ccgccgaatg
      661 cgccaccagc gcacacgcaa caagctctcg aggagcgcct cgcatcgcaa ggcgctgttc
      721 atgaacctct cgcgcgaggt gttcaaccat gagcgcatca agacgaccga ggcgaaggcc
      781 aaggccgtcg cgccggaggt cgagaagctg atcacgctgg ccaagcgggg cgacctgcac
      841 gcgcggcgcc aggcgctgtc ggcgctcggg caggacaagt tcgtggtcta caagctgttc
      901 gaggagatcg cgccccgtta cgccgagcgc aacggcggct acacgcgcat cctcaagctc
      961 ggcccgcgtc actccgacgc gaccgagatg gtctacctcg agctggtcta agcagacgac
     1021 ttccgatccc ctggatcgaa tgaaggccga gtcccgcgag tcgtggggcg tcaatccgta
     1081 cgccccgctc gcggacctgc tgcgccccgc cgcggtcgag ctcgtccgcg cgtgcgggat
     1141 cgggcccggc atgcgcgtgc tcgacgtggc ggccggcaac ggcaacgccg cggtggaggc
     1201 ggttcgcgcg ggcgcggagg ccgtcgcctc ggacctcgcg ccgcttcagg tcgagcaggg
     1261 gcgcgagcgc actcgcgccg agggcctcga catcgagtgg gtggaggccg acgccgagga
     1321 gctgccgttc gaggacgggt cgttcgacgc gtcgatgtcg gtgttcgggg cgatgttcgc
     1381 gccgcgcgtc gatcgcgccg ccgccgagct gttccgggtc gtgcgcccgg gcggcaccgt
     1441 cgggatggcg aactggcgcc cggacgggtt ccaggcgcgc ttcttcgatg tcctcaacag
     1501 gtaccgcgat ccggacccgc ccggcgtcga gccggcgtcg ctctggggcg acgaggagca
     1561 cgtgcgcgag cgcttcggcc cgcatgcgga cgaggtgcgc gtggagcccc ggatgctgcc
     1621 gtggcagtgg agctcgcttc aggagatggg ggagttcttc gccgcaacgc ggcccggcgg
     1681 cagcgccgcg gggatgagcg aggagcggcg ccagcagctc gcgatcgacg tgctcgagct
     1741 ggtgcaggag cacaacaccg cggagggcgg cgcggtgtac atcgactccg cctactcgat
     1801 cgtcgtcgcc cgccgcaaga gctgaccgcc cggctcctga tcgagtacga cggctccggc
     1861 ttcagcggct gggcccgcca gccgggccac cggacggtgc agggcgtgct cgaggaggcg
     1921 ctcgagcggg ttcggccgga gcccgcggcg ctcacggtcg cggggcggac cgacacgggc
     1981 gtgcacgcgc tcggacaggt cgcgagccat ccgggcgagc cggccctggc gcggtcgctg
     2041 aacggcgtgc tgcagcgcga cgtgcgggtg gtcgccagcg agcgggcccc cgacggcttc
     2101 gacgcgcgcc gcgacgcgct ctcgcgtacc taccgctacc gcgtccacac ccgcgaggcc
     2161 cagagcccgt tcgagcgcct gcgatcgctg cactggccgc aggcgctcga ccgcgacgcg
     2221 ctcgaccgct gcgccgcgct gctgcccggc acgcacgact tcacggcgtt caccccgacg
     2281 cagaccgacc acgtgcgctt cgagcgcgac gtgctgcgtg cggagtggtc cgtcgagggg
     2341 ccggacatgg tggcgttttg gatcgaggcc gactcgttca tgcgccacat ggtgcggatc
     2401 ctggtcggct cgatgctcgc ggtcgcgcgt ggctaccggc cgctcgactg gttcgagggc
     2461 ctgctcgcga gcgcgccgcg cagcgaggcc ggcgacaccg cccccgcgca cgggctctac
     2521 ctcgaggccg ttacctatcc tcaggacggc tcgtgaaggt ccttctcacc aacgacgacg
     2581 gcatccaggc caccggcctg aacgcgctgc gcagggccct cctcgaggtg ccgggggtcg
     2641 agctgtcggt gatcgccccg aacgcgaacc gcagcgccac cgcgcgcagc atcaccaccc
     2701 gctcgccgct gtgggtcgag gagatcgagt tcgaggacgg gaccacgggt ttcgccaccg
     2761 acggcacccc ggtggactgc gtgcgcttct ccgtgctcgg gctggtggac cgcccggagc
     2821 tgatcgtctc cggcatcaac cacggcacga acctgggcga cgacatcacc tcctccggca
     2881 cggtcgccgc ggcgctcgag ggcgtggtgc tcgggctgcc cgcgatcgcg gtctcccagc
     2941 agtccgaggg cggcgagatg gacttccgcg ccggcctgcg gttcgacttc gaggagggcg
     3001 cggcattcgt ggcgcggctg gtcgaggagc tcgagtcggt gccggtcccg gaggggacgc
     3061 tgctgaacgt gaacgtcccg gccggcgacg cgaagggcgc ccgcgtgtgt gggctcggga
     3121 ggcggatcta ccgcgacaag ctcgagctcg tggaggagga ggacggccgc aagcgctacc
     3181 gcatctacgg cgagcgcccc ggccaccacg aggaggaggg caccgacttc gccgcgctct
     3241 cggagggcct catcgcggtc accccgctgc acttcgacct caccgaccgg gcgggcatcg
     3301 agtccctgtc cgggttcgac ctcgacgggc tgatccggcc cgccgcgcaa gaggttgagt
     3361 agcgccgagg cacgcgcggc cgagctgcgc cgcgtcatcg agcagcacaa ccacagctac
     3421 tacgtcctcg acgagccgac ggtcggcgac gacgagtacg actcgctgct gaacgagctg
     3481 cgccagctcg aggccgagca cccggagctg cgcacgccgg actcgccgac gcagcgcgtc
     3541 ggggccaagc cgctcgacaa gttcgagccg gtccgccacc tccagccgat gctctcgctc
     3601 gcgaacgcgc gcaacgagga ggagctgcgc gcgtgggatg cgcgggtgcg caagctcgcc
     3661 ggcgaggagg cgccggagat cacctacggc acggagccga agatcgacgg cctggcgatc
     3721 tcgctggtct acgagaacgg tctgttcgtg cgcggagcga cccgcggcga cggcgagatc
     3781 ggcgaggacg tgacgcagaa cctgcggacg atcaaggaga tcccgctacg gatcccggac
     3841 gcaccgcctc tggtggaggt ccgcggggag gtctatctac cgcgatccgc gtttgcgcgg
     3901 ctcaatgagg aaagggccgc ggccggagag ccgaccttcg cgaacccgcg gaactccgcc
     3961 gccggctcga tccgccagct cgatccggcg ctggcggcct cgcggccgct gtcgatgtgg
     4021 tgctacggga tcggcactac cgagggcctc tcgttcgacc gccacagcga ggcgctccag
     4081 tggatgcgcg accgcggctt caaggtcgcg cccgacacga aggtgcacgc cgacctcgag
     4141 tcgctcgtgg ccgagtgccg gcgctgggag aacgaccgcg agtcactcga ctacgagatc
     4201 gacggcgtgg tggtgaaggt ggacgagctc gacctccagc gctcgctcgg cgtggtcggc
     4261 cgcgagccgc gcggggcgat cgcgtggaag ttcgcgccga tgaccgccac gaccactctg
     4321 cgcgcggtga tgtggaacgt cgggcggacc gggcacatgg tcccgttcgc caacctcgag
     4381 ccggtgcagg tgagcggcgt gaccgtgaag ctcgccacgc tgcacaacga ggaggacctg
     4441 cgccgcaagg acgtgcgcga cggcgacgag gtgatcgtga tgcgcgccgg cgacgtgatc
     4501 ccgcaggtgg tctcgccgac cgcgaaggcg cagcgctcga agaagcggag cgcgccgccg
     4561 gagccgcccg ccgagtgccc ggcgtgcg
//
LOCUS       DASVPL010000196         9523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_139637, whole genome shotgun sequence.
ACCESSION   DASVPL010000196 DASVPL010000000
VERSION     DASVPL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139637"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF517_10180"
     rRNA            complement(<1..373)
                     /locus_tag="VF517_10180"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(780..1952)
                     /gene="tyrS"
                     /locus_tag="VF517_10185"
     CDS             complement(780..1952)
                     /gene="tyrS"
                     /locus_tag="VF517_10185"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX8053351.1"
                     /translation="MTDPAAFLLRNAVDALPAGELERKLAEGRPLRVKLGIDPTAPDI
                     HLGHTVVLRKLREFQDLGHVVVLIVGDYTARVGDPSGRSALRPVLDPEDIDRNAQTFQ
                     EQAFKVLDPDRTEVRRNGEWLDMSMEELFRLARTATVAQLLERDDFAKRYSSGVPISI
                     LELLYPLLQGYDSVQVRSDVELGGTDQTFNLLLAREIQKAYGVAPQVALTMPILPGTD
                     GTQRMAKSAGNYIGVTEPAEEVFGKAMSVPDEAMAVYYELLLDRELDRDRHPAESKRE
                     LARELVARFGGGEEAARAAEAHFDRLHVTREMPGEIDDAPLPDGDPLHMPAVLASAFG
                     VSRSEARRLLTGGGVKLDGEPVDGGRLDVPAGELAGRVIQLGKRRFKRLVAPADTR"
     gene            2075..4264
                     /locus_tag="VF517_10190"
     CDS             2075..4264
                     /locus_tag="VF517_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8053352.1"
                     /translation="MDADDPAGTEHPPKRRIKKLRLALVLFGLGVLAVVSTVFGMMMA
                     VSNELPSLEDTAQFKAARNSSLVAANGKDQIAQLTDNQNRILLRPAEIAPSVKNAVIA
                     IEDRRFYTHGGVDYTGIARALWQDVKRQEAVQGGSTITQQFVKNALDAQGERSIFQKL
                     KESALAYHLERRWTKPKILTQYLNTVYFGNGAYGIEAAARTYFGGRASYGRGERIAQD
                     LAPEEAALLAGIIASPSKYDPVTNPIAAQQRRDQVLGNMLDQGMIGQVEHDRAVRTAI
                     PAASAITPPQPDSSQPYFSTWVTQQLVDRYGPGATFGSGLKIRTTLDPAMQKAAEQAI
                     AGRLGGVGPTASIVVIDNKTGGVRAMVGGDNFERRPFNLATNGKRQPGSAIKPFILAA
                     ALQNGTSPNSVWSSRPKTFPYKEPNGKKDIFVVKNYANSYSGTTTLARATAQSDNSVY
                     AEIGMTVGRKKVAKIANEMGIRTKLSTNPAMLLGGLREGVTPLEMAFAYSTIANRGRR
                     VSGSLASYPGGPVAIEKVENARGKTRDKNKSRSKRVYSEEVGEQMRSLLHGVVVGGTG
                     TKAQVGEWAAGKTGTTENYGDAWFVGFTDRYTAAVWVGYPDRVKYMTTHYHGQPVAGG
                     TFPTEIWHDFMSAAIRIDDARGKGKDEGEAGEVPPPSAAPTTPAPGGPAAPAPQQTVP
                     ETPQPQTPQQPQQPAPQPTPAPQPAPEPPRGGGGGQGGGTSPGTAAQ"
     gene            complement(4324..5586)
                     /locus_tag="VF517_10195"
     CDS             complement(4324..5586)
                     /locus_tag="VF517_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8053353.1"
                     /translation="MRRVAVAAALLVLVPASAHAQAPGPEGYCEPQNSQYDPQDLQRP
                     DAPPVELPPGFTRGRISIGRYSAPTIEAGPRDSREAIVLLHGNPGRSLDWLGVLRSVP
                     HGTRVIAFDLIGFGTADKPYDFPYDLETTTPLLAQTFRELGIDRVHLVGHDIGSIVGI
                     DWAAANPDRLASAVLFAGGIFIGYQDHHFARVWRTPGVGEQMMYGTDREGFVSVIQAH
                     SPRPLPREFLDRDYDVYDRATRCAVLVSYRREGDIDARARQHAEALRPHDRPALVVFG
                     DRDPFVPSYIAANSRQAFPHADVHVYENSGHWPFVDEEERTVGLMRDFFRSHVVEQAG
                     AKIRLRVTPRHPRRGRRTRFRVRVVVGEARRPLPGALVTLLGRRARTDAAGRAQLVAT
                     PRATGLVKATALKESLLAGRKTIRVLAR"
     gene            complement(5607..6224)
                     /locus_tag="VF517_10200"
     CDS             complement(5607..6224)
                     /locus_tag="VF517_10200"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX8053354.1"
                     /translation="MTPADRAFYDRSVHDVAHDLVGCILRHGPTAARIVETESYHMEE
                     PACHAFAGLTPRTEVLFGEPGLAYVYRSYGIHALLNAVAEPEGVGAAALIRAVEPIEG
                     IEVMRDRRGPVRDLDLCSGPGKLTQAMGIWLDLNGTPLAGEGPIEILPPDAAERPPKI
                     VRGTRIGITKAVDLPWRFCDATSRHVSRPWPPAMRTRRTGARAAA"
     gene            complement(6221..7591)
                     /gene="argH"
                     /locus_tag="VF517_10205"
     CDS             complement(6221..7591)
                     /gene="argH"
                     /locus_tag="VF517_10205"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX8053355.1"
                     /translation="MSRFAEGQDPLFQAINSSVAFDYRLAPYDLEQSGAHARMLARCG
                     IIEEADLAEIERGLELVRQEIDSDAFEVHPGDEDIHMAIERRLTELVGPVGGKLHTAR
                     SRNDQVATDVAMFVRAHSLSARELTRDVMIALIDLAERHLDWPMPGYTHLQRAQPVYL
                     SHHLLAYFWKLRRDMARFEMCLGGADELPLGAGALAGVSFSTDRRGVAAELSFGSVAE
                     NSIDAVSNRDFVLDYLGAASICATHLSQLGAEIVLWSSEEFGYCELPDSFSSGSSIMP
                     QKKNPDAAELLRAKAPRVVAHLAGLHGVLHALPLTYNKDLQEDKEHLFDAVDTIELSL
                     LVAREMLRGISFRRDRLADAAADEMLGATDIADLLVRRGVPFREAHGIVGGLVRHAID
                     TGRTLSELPAAEVAERAPQIDAGLAETLRRETVLESKVSEGGTALASVREQLERARKV
                     LQDSPA"
     gene            7629..7985
                     /locus_tag="VF517_10210"
     CDS             7629..7985
                     /locus_tag="VF517_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053356.1"
                     /translation="MGKRGRRRSRDEPGSTTASEYRDADGNVLALRDQLSAGTVAKLR
                     EPVGGAAATSDDLWRRRTEMLFERLAVSWEIAGLPLTTQKELLGRYRMADGETQSWVR
                     STIEEHLRKHQPELVD"
     gene            7982..8947
                     /locus_tag="VF517_10215"
     CDS             7982..8947
                     /locus_tag="VF517_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8053357.1"
                     /translation="MTRALVTGATGKVGGAVARALLARGDSVQALVRSPERAARLLPA
                     EVELVRGDVTDARSVEAAVAGCSLVFNAMGLPEQWTADPDVFDRVNARGTETVVRAAR
                     AAGVARVVHTSTIDVFHAGRGERFDESRVADYPKGTIYERSKQRAEELALAARDGMEL
                     VICNPAGVYGPTPASSVSIDDDLFAPLVAGALPALPPGGLGLCFDAGVAEGHLLAAER
                     GRDGERYILCDRHVTLRSLAREVVGVARRGRVPPTLPAPVARAMAEAGEALARTAGVS
                     PLLARGQLHFFRWNAAPDSTKAQEELGWEPTALEDGLKAAVAALG"
     gene            8940..>9523
                     /locus_tag="VF517_10220"
     CDS             8940..>9523
                     /locus_tag="VF517_10220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8053358.1"
                     /translation="MGEGSAGPPGGGADGGAAGPGSVEGSAAGPGGAKPGAAGPPGGE
                     LRLRAARREDADAVALLVHSTAEAMYERFAGDRDSSVRVLRAGFRRAGTGASGDVVTV
                     AEVGGAVAGAMAAFPAQEIERRARRFMRLLLRRTPPWTWRETYRLYRLGAEVAPPPPP
                     SSFYVDSLATDPRHRRRGVARALLAEAERRAREEG"
BASE COUNT         1374 a         3242 c         3614 g         1293 t
ORIGIN      
        1 gcaagattcc ccactgctgc ctcccgtagg agtctgggcc gtgtctcagt cccagtgtgg
       61 ctgatcgtcc tctcagacca gctacccatc gcagccttgg taggccgtta ccccaccaac
      121 aagctaatgg gccgcgggcc catcccgatg cgaaggccga agctatcttt cctctcacca
      181 ctgatgtaat gagggttcat ccggtattag cccgagtttc ctcgggttgt cccggtcatc
      241 ggggcaggtt acccacgtgt tactcacccg ttcgcggctc tgccacgggc cgaagcccgc
      301 ttcgtcgctc cacttgcatg tgttaagcac gccgccagcg ttcgtcctga gccaggatca
      361 aactctccgt aaagaactgg cgtgtcacgc gatcgtcccg agggacgacc ggtttcactg
      421 tctatatgga aagctgtcat gtccgtactg actgcggacc cgagtacacc gtgggtccgc
      481 tcggacgtga cggggtgatt ctggaccgcc gctccctcgc cggcgccacg agtggcacct
      541 tgaaaaggga cagcgggtcg aacctggacg cgactcgatc gaggccgaag ccccggtcgg
      601 tcgcacacgc tgttgagttt tcaaagaccg ccgcgccctc gagacggatg ctccgcctcc
      661 catcgggtgg ggactccacc ctgctgggcg acccgcgagt atagcgccga accgggcggt
      721 caggcaaccc ttcgcgaggc ccccgacggt cgtgttccgg ccagtcggga ggcgggagct
      781 tagcgggtat cggcgggtgc gaccagccgc ttgaaacgcc gcttcccgag ctggatcacg
      841 cgtccggcca gctcgccggc ggggacgtcg aggcggccgc cgtcgaccgg ctccccgtcg
      901 agcttcacgc ccccgcccgt gagcagcctg cgcgcctccg agcggctgac gccgaaggcg
      961 gacgccagca ccgccggcat gtgcagcgga tcgccgtccg gcagcggcgc gtcgtcgatc
     1021 tcgccgggca tctcgcgggt gacgtggagg cggtcgaagt gggcctcggc cgcacgcgcc
     1081 gcctcctcgc cgccgccgaa gcgggccacg agctcgcgcg ccagctcgcg cttggactcc
     1141 gccgggtggc ggtcgcggtc gagctcgcgg tccagcagca gctcgtagta gaccgccatc
     1201 gcctcgtccg ggacgctcat cgccttgccg aagacctcct cggccggctc ggtcacgcct
     1261 atgtaattgc cggcggactt ggccatgcgc tgcgtgccgt cggtccccgg gaggatcggc
     1321 atcgtgagcg cgacctgcgg cgccacccca tatgccttct ggatctcgcg cgcgaggagg
     1381 aggttgaagg tctggtcggt gccgccgagc tcgacgtcgg agcggacctg aaccgagtcg
     1441 tagccctgga gcaacgggta gagcagctcg aggatcgaga tcgggacgcc ggaggagtag
     1501 cgcttggcga agtcgtcgcg ctcgaggagc tgggcgacgg tggccgtgcg cgcgagccgg
     1561 aacagctcct ccatcgacat gtcgagccac tcgccgttgc ggcgcacctc ggtgcgatcg
     1621 gggtcgagca ccttgaaggc ctgctcctgg aaggtctgcg cgttgcggtc gatgtcctcg
     1681 gggtcgagca ccggccgcag ggccgagcgg ccgctggggt cgcccacgcg cgccgtgtag
     1741 tcgccgacga tcagtaccac gacgtgtccg aggtcctgga actcgcgcag cttgcgcagc
     1801 acgacggtgt gtccgaggtg gatgtcgggc gccgtcgggt cgatgccgag cttcacgcgc
     1861 agcgggcggc cctcggccag cttgcgctcg agctcccccg cgggcagcgc gtcgaccgcg
     1921 tttcggagca ggaaagcggc tgggtcggtc acggcgccga tgctagtgtc gcggcatcgc
     1981 cgcaggcact ccgggtgccc tcccggaccg ctgctggcct gagggaatgc aggaatcgcc
     2041 gccgataccg ctgcgaccgc cacgccgacg tcgcgtggat gccgacgacc ccgcgggcac
     2101 cgagcacccc cccaagcgtc ggatcaagaa gctccgtctc gccctcgtgc tgttcgggct
     2161 tggcgtgctc gccgtggtct cgacggtctt cgggatgatg atggccgtct cgaacgagct
     2221 gccgtcgctc gaggacaccg cgcagttcaa ggccgctcgc aactcgtcgc tcgtggccgc
     2281 gaacggcaag gaccagatcg cgcagctcac ggacaaccag aaccggatcc tgctgcgccc
     2341 ggccgagatt gccccgagcg tcaagaacgc ggtgatcgcg atcgaggacc ggcgcttcta
     2401 cacgcacggg ggtgtcgact acacggggat cgcacgcgcg ctgtggcagg acgtgaagcg
     2461 ccaggaggcg gtgcagggcg gctcgaccat cactcagcag ttcgtgaaga acgcgctcga
     2521 cgcccagggc gagcgctcga tcttccagaa gctgaaggag tccgcgctcg cctaccacct
     2581 cgagcggcgc tggaccaagc cgaagatcct gacccagtac ctgaacacgg tctacttcgg
     2641 gaacggcgcg tacgggatcg aggcggcggc gcgcacgtac ttcggcggtc gcgcgagcta
     2701 cggccgcggc gagcggatcg cgcaggacct cgcgccggag gaggccgcgc tgctcgcggg
     2761 catcatcgcc tcgccgagca agtacgaccc ggtgacgaac ccgatcgccg cccagcagcg
     2821 ccgcgaccag gtgctcggga acatgctcga ccagggcatg atcgggcagg tcgagcacga
     2881 ccgcgcggtg cgcacggcga tcccggcggc gagcgcgatc acgccgccgc aaccggactc
     2941 gtcccagccc tacttctcca cctgggtgac ccagcagctc gtcgaccgct acgggccggg
     3001 cgccacgttc ggcagcgggc tgaagatccg cacgacgctc gacccggcta tgcagaaggc
     3061 ggccgagcag gcgatcgcag gccggctcgg cggggtcggc ccgaccgcct cgatcgtggt
     3121 gatcgacaac aagaccggcg gcgtgcgcgc gatggtgggc ggtgacaact tcgagcggcg
     3181 cccgttcaac ctggccacca acggcaagcg ccagcccgga tcggccatca agccgttcat
     3241 cctcgccgcg gcgctccaga acgggacatc gcccaactcg gtgtggtcct cgcggccgaa
     3301 gacgttcccg tacaaggagc cgaacggcaa gaaggacatc ttcgtggtca agaactacgc
     3361 gaactcgtac tcgggcacca ccacgctggc gcgcgccacg gcgcagtccg acaactccgt
     3421 gtatgccgag ataggcatga cggtgggccg caagaaggtc gcgaagatcg ccaacgagat
     3481 gggcatccgc acgaagctct ccaccaaccc ggcgatgctg ctcggcggcc tgcgcgaggg
     3541 cgtgactccg ctcgagatgg cgttcgcgta ctcgacgatc gccaaccgcg ggaggcgcgt
     3601 gtccgggtcg ctcgcctcgt acccgggtgg tcccgtggcg atcgagaagg tggagaacgc
     3661 gcgcgggaag accagggaca agaacaagtc gcgctcgaag cgggtgtact ccgaggaggt
     3721 cggcgagcag atgcgctcgc tgctgcacgg ggtggtcgtg ggcgggacgg ggaccaaggc
     3781 gcaggtgggc gagtgggcgg ccggcaagac cggcacgacc gaaaactacg gcgacgcgtg
     3841 gttcgtcggc ttcaccgacc gctacacggc ggccgtgtgg gtcggatatc cggaccgggt
     3901 gaagtacatg acgacccact accacgggca gccggtcgcg ggcgggacgt tcccgaccga
     3961 gatctggcac gacttcatgt cggccgcgat caggatcgac gatgcgcgcg gcaagggcaa
     4021 ggacgagggc gaggccggcg aggtgccgcc gccgtcggcc gctcccacca cccccgcgcc
     4081 cgggggccca gccgcgccgg cgccccagca gacggtgccc gagaccccgc agccgcagac
     4141 gccgcagcag ccccagcagc cggccccgca gccgactccg gcgcctcagc ccgcgccgga
     4201 gccgccgcgt ggtggcggcg gcgggcaggg cggcgggaca tcgcctggca cggcggctca
     4261 gtagggatcg ttcgacaatc ctgactcttg ggtcgtatgg accctcaagt caggaatgcc
     4321 gggtcagcgc gcgagcacgc ggatggtctt gcggcccgcc aggagcgact ccttcaacgc
     4381 ggtcgccttc acgagtccgg tggcgcgcgg cgtggccacg agctgcgcgc ggcccgcggc
     4441 gtccgtgcgc gcgcggcggc cgagcagggt gaccagggcg cctggcagtg ggcgccgcgc
     4501 ctccccaacc acgacccgca cccggaaccg ggtccggcgg ccgcggcggg gatgccgcgg
     4561 ggtgaccctc agccggatct tcgctccggc ctgctcgacc acatgggagc ggaagaagtc
     4621 gcgcatcagc ccgaccgtgc gctcctcctc gtccacgaac ggccagtgtc cgctgttctc
     4681 gtacacgtga acatccgcat gcgggaacgc ctgccggctg ttcgcggcga tgtaggaggg
     4741 aacgaagggg tcgcggtcgc cgaagacgac gagcgcgggc cggtcgtggg gccgcagtgc
     4801 ctcggcgtgc tggcgcgcgc gggcgtcgat gtcgccttca cgccgatacg agacgaggac
     4861 cgcgcagcgc gtcgcacggt cgtagacgtc gtagtcgcgg tcgaggaact cgcgcggcag
     4921 ggggcgcggg ctgtgggcct ggatgacgct cacgaagccc tcccggtcgg tgccgtacat
     4981 catctgctcg ccgacgccgg gcgtcctcca cacgcgtgcg aagtggtggt cctggtaacc
     5041 gatgaagatg ccgccggcga acagcaccgc gctggcgagc cggtccgggt tcgcggcggc
     5101 ccagtcgatg ccgacgatgc tgccgatgtc gtgccccacg aggtgcacgc gatcgatccc
     5161 gagctcgcgg aacgtctgcg cgagcagcgg cgtggtggtc tccaggtcgt acgggaagtc
     5221 gtagggctta tcggccgtcc caaagccgat caggtcgaac gcgatcacac gggtcccatg
     5281 cggcaccgag cgcagcacgc cgagccagtc cagcgaccga cccggattgc cgtgcaggag
     5341 gacgatcgcc tcgcgggagt cgcgcggccc ggcctcgatg gtcggcgccg agtagcggcc
     5401 gatcgagatc cggccgcggg tgaagccggg cggcagctcg accgggggcg cgtccgggcg
     5461 ctggaggtcc tgggggtcgt actgggagtt ctgcggctcg cagtagccct cggggccggg
     5521 ggcctgcgcg tgcgcggagg cgggcacgag tacgagcagg gcggctgcaa cggcaacgcg
     5581 gcgcatcccg tcctggattg tgagggtcag gcagcggcgc gtgcgccggt gcggcgggtg
     5641 cgcatcgccg ggggccaggg gcgcgagaca tggcgcgagg tggcgtcgca gaagcgccag
     5701 gggaggtcga cggccttggt gatcccgatg cgggtgccgc ggacgatctt cgggggccgc
     5761 tcggcggcgt cgggcgggag gatctcgatc ggcccctccc ccgccagcgg cgtgccgttc
     5821 aggtcgagcc agatgcccat cgcctgcgtg agcttgccgg ggccggagca caggtcgagg
     5881 tcgcggaccg gcccgcggcg gtcgcgcatg acctcgatgc cctcgatcgg ctcgacggcg
     5941 cggatcagcg cggccgcccc gacgccctcg ggctcggcca ccgcgttcag cagggcgtgg
     6001 atcccgtacg agcggtacac gtaggcgagg cccggctccc cgaacagcac ctcggtgcgc
     6061 ggcgtgagcc cggcgaaggc atggcaggcg ggctcctcca tgtggtagct ctcggtctcg
     6121 acgatgcggg ccgcggtggg gccgtggcgc aggatgcagc cgacgaggtc gtgcgcgacg
     6181 tcgtggaccg aacggtcgta gaaagcgcgg tcggccggcg tcacgccggc gagtcctgga
     6241 gcaccttgcg ggcgcgctcg agctgctcgc gcacggatgc cagcgccgtg ccgccctcgg
     6301 agaccttcga ctcgagcacg gtctcgcgcc tgagcgtctc ggcgaggccg gcgtcgatct
     6361 gcggggcgcg ctcggccacc tcggcggcgg gcagctcgga cagggtccgt ccggtgtcga
     6421 tcgcgtggcg gacgagcccg ccgacgatgc catgcgcctc gcggaagggc acgccgcggc
     6481 gcacgagcag gtcggcgatg tcggtggcgc cgagcatctc gtcggcggcg gcgtcggcca
     6541 gccggtcgcg gcggaaggag atcccgcgca gcatctcgcg ggcgaccagg agcgacagct
     6601 cgatcgtgtc gaccgcgtcg aagaggtgct ccttgtcctc ctggaggtcc ttgttgtagg
     6661 tgagcgggag ggcgtggagc acgccgtgga gcccggccag gtgggcgacc acgcgcggcg
     6721 ccttcgcgcg cagcagctcg gccgcgtccg ggttcttctt ctggggcatg atgctcgacc
     6781 ccgaggagaa cgagtcgggc agctcgcagt agccgaactc ctcgctcgac cagagcacga
     6841 tctcggcgcc gagctgcgag aggtgcgtgg cgcagatcga cgcggcgccg aggtagtcga
     6901 gcacgaagtc gcggttcgat acggcatcga tcgagttctc cgccaccgag ccgaagctca
     6961 gctcggcggc aacgccgcgc cggtcggtcg agaagctcac cccggcgagg gcaccggcgc
     7021 ccagcggcag ctcgtcggcc ccgccgaggc acatctcgaa gcgggccatg tcgcggcgca
     7081 gcttccagaa gtaggccagc aggtggtggg agaggtagac cggctgggcg cgctggaggt
     7141 gcgtgtagcc gggcatcggc cagtcgagat ggcgttcggc gaggtcgatc agggcgatca
     7201 tcacgtcgcg ggtgagctcg cgggcgctga ggctgtgggc gcggacgaac atcgccacgt
     7261 cggtcgcgac ctggtcgttg cgcgagcggg cggtgtgcag cttgccgccg accggcccga
     7321 cgagctcggt cagcctccgc tcgatcgcca tgtggatgtc ctcgtcgccc gggtggacct
     7381 cgaaggcgtc gctgtcgatc tcctggcgga ccagctcgag gccgcgctcg atctccgcga
     7441 ggtcggcctc ctcgatgatc ccgcaacggg cgagcatccg cgcgtgggcg cccgactgct
     7501 cgaggtcgta cggcgcgagc cggtagtcga acgccaccga cgagttgatg gcctggaaca
     7561 gcgggtcctg gccctcagcg aagcgggaca tggcgggcca gtctacgggc ggcggtagcc
     7621 tcgcccggat gggaaagcgg ggacggcggc gcagccgcga cgagccgggc tccacgacgg
     7681 cgagtgagta ccgcgacgcc gacggcaacg tgctcgccct gcgcgaccag ctctcggccg
     7741 ggaccgtggc gaagctgcgc gagccggtgg gcggagcggc ggcgacgagt gacgacctct
     7801 ggcgccggcg gacggagatg ctgttcgagc ggctggcggt gagctgggag atcgcgggcc
     7861 tgccgctgac gacgcagaag gagctgctcg gccgctaccg gatggccgac ggcgagacgc
     7921 agagctgggt gcgctccacc atcgaggagc acctgcgcaa gcaccagccc gagctggtgg
     7981 attgacccgc gcactggtca ccggggcgac cgggaaggtc ggcggggcgg tggcgcgggc
     8041 gctgctggcg cgcggcgact cggtgcaggc gctggtgcgc tcgccggagc gggctgcgcg
     8101 gctgttgccc gcggaggtgg agctggtgcg cggggacgtg accgacgcgc gctcggtcga
     8161 ggcggcggtg gcgggctgct cgctcgtctt caacgcgatg gggctgccgg agcagtggac
     8221 cgccgacccc gacgtgttcg accgcgtgaa cgcgcgcggg accgagacgg tcgtgcgcgc
     8281 ggcgcgggcc gcgggggtcg cgcgcgtggt gcacacgagc acgatcgacg tgtttcacgc
     8341 ggggcgcggg gagcggttcg acgagtcacg cgtggccgac taccccaagg gcacgatcta
     8401 cgagcggtcg aaacagcggg ccgaggagct cgcgctggcg gcgcgcgacg gcatggagct
     8461 cgtgatctgc aacccggcgg gggtgtacgg gccgacgccg gcctcgagcg tctcgatcga
     8521 cgacgacctg ttcgcgccgc tcgtcgccgg cgcgctgcct gcgctgccgc ccgggggcct
     8581 cgggctgtgc ttcgacgccg gcgtggcgga gggtcacctg ctcgcggccg agcgcgggcg
     8641 tgacggcgag cgctacatcc tctgcgaccg ccatgtgacc ctgcgttcgc tcgcgcggga
     8701 ggtcgtggga gttgcgaggc gcggacgcgt gccgccgacg ctgcccgcgc ctgtggcgcg
     8761 ggcgatggcc gaggccggcg aggcactggc gcgcacggcg ggcgtgtcgc cgctgctggc
     8821 tcgcgggcaa ctgcacttct tccgctggaa cgccgcgccg gactccacga aggcgcagga
     8881 ggagctcggc tgggagccga ccgcgctcga ggacgggctg aaggcggcgg tggcggcgct
     8941 tgggtgaggg cagcgcgggg ccgccgggtg gcggtgccga cggcggcgcg gcggggccgg
     9001 gcagcgtaga gggcagcgcg gccgggccgg gcggcgcgaa gccgggcgcg gcggggccgc
     9061 cgggtggtga gcttcgactc cgcgccgcgc gacgcgagga cgccgatgcc gtggcgctgc
     9121 tcgtccactc caccgccgag gcgatgtacg agcgcttcgc cggcgaccgt gacagctcgg
     9181 tccgggtgct gcgggcgggg ttccggcgcg cgggcaccgg ggcgagcggc gacgtggtca
     9241 cggtcgccga ggtgggcggc gcggtcgcgg gcgcgatggc cgccttcccc gcgcaggaga
     9301 tcgagcgccg ggcgcggcgg ttcatgcgcc tcctgctgcg gcggaccccg ccgtggacct
     9361 ggcgcgagac ctaccggctc taccggctcg gagccgaggt cgcccctccc ccgcccccgt
     9421 cctcgttcta cgtggactcg ctggcgacgg acccgcgcca ccgccgccgc ggcgtggccc
     9481 gcgcgctgct ggccgaggcc gagcgccggg cgcgcgagga ggg
//
LOCUS       DASVPL010000197        17992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_297489, whole genome shotgun sequence.
ACCESSION   DASVPL010000197 DASVPL010000000
VERSION     DASVPL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17992
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297489"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /gene="tmk"
                     /locus_tag="VF517_10225"
     CDS             complement(<1..329)
                     /gene="tmk"
                     /locus_tag="VF517_10225"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX8053359.1"
                     /translation="MPGLFVTFEGIDRSGKPTQARMLVAALGAERALAVREPGGTELG
                     ERVREVLKGSIPLDARAETLLFAAARAQLVADVIRPALQADRVVVSDRFLDSSLAYQG
                     DARGLGV"
     gene            387..1313
                     /locus_tag="VF517_10230"
     CDS             387..1313
                     /locus_tag="VF517_10230"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8053360.1"
                     /translation="MRALVTGGAGFIGSNVVDGLLAQGDEVTVVDDLSTGKRENLGQA
                     LGNGAQLVEADIRDAEALKDIFAQARPEVVFHLAAQIDVRKSADDPAFDARVNVEGTI
                     NVLQAARASGARRLVNTSTGGAIYGEAQVIPAPESHPNAPEAPYGQSKHAAEGYCELF
                     RRLFGLSTVSLRYGNVYGPRQDPLGEAGVVAIFCGKIRSGERPVVFGDGLQTRDYVHV
                     SDVVAANLTAAGSDSYGPINIGTGVEASVLDLVEALAPHASSDFTPDHQPERPGEVRH
                     IALDASRARAELGWEPRMALTEGIEQTLASVG"
     gene            1349..1519
                     /locus_tag="VF517_10235"
     CDS             1349..1519
                     /locus_tag="VF517_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053361.1"
                     /translation="MTAAIICAAVGLVLLGLGVAWLSGAIGGSGEGAATDWRERTADT
                     AADFRDWLRTGR"
     gene            2390..5269
                     /locus_tag="VF517_10240"
     CDS             2390..5269
                     /locus_tag="VF517_10240"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX8053362.1"
                     /translation="MPQSPSSAADITARRTGQGVMVERRYTEPGVHPFEQIEWEIRDA
                     VIGNPEKPAFEQLGVEFPKSWSQNATNIVAQKYFRGRLGSDERESSVKQMIGRVAGTI
                     AGWGRELGYFATDEDGDAFEAELTYILLHQQAAFNSPVWFNVGFEEKPQCSACFILSV
                     EDTMESILGWNTKEGMIFRGGSGSGINLSNIRSSKEYLSKGGLASGPVSFMRGADSWA
                     GTIKSGGKTRRAAKMVVLDIDHPDIVDFIWCKASEEEKAAALRDAGFDMSIDGEGFTS
                     IQYQNANNSVRVTDEFMHAVEAGSDWHTIARVSREPVDTYDARDLMRQIADAAWRCAD
                     PGVQYDTTINDWHTCPASGRINASNPCSEYMHVDDSACNLASINLMKFRRPDGSFDAS
                     AFEHVVDVMILAQEIVVGPSSYPTEGIGENARAFRQLGLGYANLGALLMADGLPYDSD
                     EGRSYAAAITALMTGRAYRKSAEVAGAIGPYERYPENREAHNHVMEKHRAASYELSDT
                     ECRDAELLAAARRSWDEAVALGLMHGYRNAQATVLAPTGTISFLMDCDTTGVEPDFSL
                     VKFKELVGGGQMTIVNKTVPLALERLGYGEAEVDQIGAWIQEHGTILGAPGLKDEHLP
                     VFDVAVGQRAISHMGHIQMMGAVQPFISGAISKTVNMPAETTVEDIADAYVQAWKLGV
                     KALAIYRDGSKTAQALRTDAHKGKDAPATAEVVEETPRPARRKMPKERQSLTHKFSIA
                     GHEGYITAGMYEDGTVGEIFLTDIGKEGSTLRGMMNAFATSVSISLQYGVPLETLVRK
                     FSYMRFDPEGATGNPEIPFAKSMPDYIMRWLASRFLDVDCQEELGIMTHEVRARKAAQ
                     EALMRGDTAAPVVEVEPTNSGGNGSGNGHANGGTNGGNGSPKASAGNGGTKPAAAAFT
                     DEPPVIPARLQGLDLGPACGQCGGMMQRTGSCYTCSSCGNNTGCG"
     gene            5460..5897
                     /locus_tag="VF517_10245"
     CDS             5460..5897
                     /locus_tag="VF517_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053363.1"
                     /translation="MNWIVIGVVLLLAALFEVWVELIAQNHPWRVAIVGGVVFLLVGA
                     ILFLWPSGPLITLTLLLAGAWAWALVAGLDARDHELGYYCRYGAQTQTELDRCMSSIN
                     TDEIEELDTPAARFAVGVRTECGPGSGRFCREAAKDAAIEYGG"
     gene            5972..6634
                     /locus_tag="VF517_10250"
     CDS             5972..6634
                     /locus_tag="VF517_10250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX8053364.1"
                     /translation="MSERLVDISHPIADGTITYPGLPAPEISEHLTRDEAEEHYAPGV
                     RFHIGRICMIANTGTYVDSPFHRFEDGVDLAGLELRRLAGRPGIVVDPPLDDGRAIEA
                     VAFHDLDLADRAVLVRTGWDRHWGTPEYGVGYPFLTAAAGEFLLEAGAAIVGIDSLNV
                     DDDTDPQRPVHTTLLGAGIPICEHLCNLTALPTWGFRFHAAPVKVEGLGTFPVRAYAE
                     LD"
     gene            6694..7197
                     /locus_tag="VF517_10255"
     CDS             6694..7197
                     /locus_tag="VF517_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053365.1"
                     /translation="MPDASDEANDIVDEVLDALMPNANYWAPRTIDDGAAPAPEQVRG
                     KAGGDGEDRVRYFAGPEALEVLAWIATSVVVPLIVGVASNRIDARLQAGSVPEPPRPR
                     GQTEAMAAVADAIARRQPHTDDRDSVELAEEHVRLLLIDYGWPAKEALSDATEIVGRV
                     SRRLRSQ"
     gene            7194..8228
                     /locus_tag="VF517_10260"
     CDS             7194..8228
                     /locus_tag="VF517_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053366.1"
                     /translation="MKPTSADSAPRELSRRRDHVLVKSAAGSQHVRNEFLRRRLQAVA
                     AGLPETPARDPKDDAKSQAPALKRVEGSSTGARIFAPQFGNELSASDHAALWEDFVAR
                     WFGDDNEDATDELMRAVGDAIMSRDRDLVLEILDALWRDRWILDQTLAAWLREWSFSP
                     QFGDFRARVRDAPEELDRAFDELRGAIAAAQDALDLDSFLARLHTLLPIAHYARRVAD
                     VADHPDAAKHSRFSEEAWVDATGVITRAVLGAAENKPGDMWVRSRVDALTPVRPEPRG
                     LRRSLRYYVARLEFPRVWVDAVLEWQAGGGAPPSWLTTYDDLTLASLAVTAFPGGSSA
                     AFRRPPAPSG"
     gene            complement(8135..9349)
                     /locus_tag="VF517_10265"
     CDS             complement(8135..9349)
                     /locus_tag="VF517_10265"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HEX8053367.1"
                     /translation="MASRRRTGTADLPLHGGRAPAWLFQRMTRLSREIVVFMASELGT
                     AEILRRLSDPHWFQAFACVLGFDWHSSGVTTTATGALKEGVRGLEHELGFFVAGGKGA
                     TSRRTPSEIERFCERLGRDPAPLVRASKVSAKVDNTAVQSGHQLYHHSFMFLADGSWA
                     VIQQGMSDETKSARRFHWLGESVDSFVEEPHAAVCDDARGDVLVNFVAAESADARAAA
                     VELAASDPKRSLDAIARASELVMPRRHELKLDDVNPAYIDRILLKTYAAAPSSFEELL
                     GIQGVGPKTLRSLALVSELVYGTRASTRDPARYAFAHGGKDGTPFPVDRATYDRTVSI
                     LESALNGARGVERSERVKAFRRLAKFAEPGEGAPGPARSAQPDGAGGRRNAALDPPGN
                     AVTASDASVRSS"
     gene            9375..9770
                     /locus_tag="VF517_10270"
     CDS             9375..9770
                     /locus_tag="VF517_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8053368.1"
                     /translation="MATIDDLLERAREDLQRVTPEEAAAAVAERDALLLDIRADSQRA
                     ADGTVPQARFVARNVLEWRLDPACEHRDPDLASYERPVILMCNEGYQSSLAAATLREL
                     GVDATDMVGGFQAWRDAGLPTATDEPDTA"
     gene            complement(9771..10712)
                     /locus_tag="VF517_10275"
     CDS             complement(9771..10712)
                     /locus_tag="VF517_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8053369.1"
                     /translation="MTVSTATAVPRTRGELEGDEALETLLRTGRRKLLVDTIARFRAA
                     DAFSHSRALAFQLTLTLVPALIAVVGLAAALDQDSFRRVVEALVTDLAPGAAGEILTD
                     ALRQGSGAAARESGETALVGGFVAALIAGTQAMAQIERGANRIYGTERDRPFPRKYGV
                     ALVIALSAGVLAVLSLVLLVGGSAIRDAAGWSDGFDSFWAIVRWPLGLAFVVASVSIL
                     FVYSPRRHQPEPSWLAFGAGASALLWLVFMGVLALYIDVAESFGQTYGPLAGTIGVLL
                     WAFLTSVALFGGLAFAAQLEAVRAGVESPRVERDENA"
     gene            10804..11343
                     /locus_tag="VF517_10280"
     CDS             10804..11343
                     /locus_tag="VF517_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8053370.1"
                     /translation="MTKRQVEDPVGRQRLAFELGTNDDGTETVLVECWVDPGGGVPPH
                     IHPHQTEVFEVVEGEMTFTAGRTKQVARAGETVTVPPGTRHAYENRSASSAYMRCVAT
                     PAAGLQDFLETTAQLSRQGHIMRIGPLRGPGSLRGLPKLAAMLERHRENTVILMPPPL
                     VQRLVLFPLARFADRSRRG"
     gene            11366..14197
                     /locus_tag="VF517_10285"
     CDS             11366..14197
                     /locus_tag="VF517_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8053371.1"
                     /translation="MERSGTGSPIADLAGDKTLLEREHELASLDVLIAAAAERQARVA
                     VVEGRAGIGKSRLIAAAREHAAASATRVLSARGTELEREFPYGVVRQLFEPLRSDPDE
                     WERLLEGAAASARPLFEAPSAADGDGQVRDASFAALHGLYWLVVNLTAETPLMLAVDD
                     LHWCDRPSLRFLAYLAPRLEGLPLLLVTGLRTGEAPTDPVLLDDVVNGPAATAVHPGP
                     LSAEAVTAMIGARLGPEVDERFAAACLESTAGNPLLLGQLISSLAAEGVVPDAGNAEK
                     VRAIGPRAVSRTVLLRLSRLSPGAMAVARAVAVLGENAELAAIAALAELDETAVAEAT
                     ASLARADILRHDLQLGFVHPLVHDAVYQDLPPGERELQHARAARVLLDGGAAPDQVAT
                     HLLMTPRRGEEWVVDLLVGAARSAVRKGATDSAIAYLRRALEEPPPEERRADLVYELG
                     FTESNASAPDAVGHLRQAYELCSDPRRRADSAFWLSRLLMLLGDPDQAAAIAAEAIAQ
                     TPAELEDERQALQAQQLTVAFWGGGDLSALDDPDSPPPRVSGSGPGAKMLLAHTAVAW
                     VVNNRPAAETCAIALDSLAGGVLIEAENGYFTVGAAVPLVLAERREGTEVWDACFADA
                     HRRGSLFGAMGAHMWHGWTLIRLGNLPEAEASLRTALTEMEMWGNPRVEYTLGFLAWA
                     LIERGRLDEARTALERVPLEPVPTWGGTHWRRSRVELLLAEGRPEEALAALDEVSGSV
                     FARNPALFPWRTHRALALDALGRTDEAIALAEEELAPARQWGAPATVARTLRILGTLQ
                     RDEGLPRLEEAAALLERSTDRLEHAKALTALGSTIRRARQPTEAREPLRRALELAAAC
                     GADGLVEHVRAELAAAGAQPRTTALSGVEALTASERRVAGLAAEGQTNRDIAQVLYVT
                     PKTVEVHLSNAYRKLGIRSRRELSGAMAT"
     gene            14194..17001
                     /locus_tag="VF517_10290"
     CDS             14194..17001
                     /locus_tag="VF517_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8053372.1"
                     /translation="MSDPLLERERERATLHRLLDAAAGQQAGLALVEGRAGIGKSRLL
                     ADLREHAAAEGIRVVSARSTELEQGFPYGVVRQLFEPLRATDPERWDAWLAGSADPAR
                     IVFETPVAGPDADELRDATFATLHGLYWLTVNLTADGPLLLAVDDLHWCDRASLRFLA
                     YLAPRLEGLPVLVATGLRTGEPGTDPALLAEIVSSPAAVAVQPAPLGSESVGELVLAR
                     LGAEADERFTAACLDATGGNPLLLSQLISSLEAEGVKPDAEHVDVVRQIGPRAVSRTV
                     LLRLSRLPAAAGAVARAVAVLGESAELRTVAAHAELDQAEAAEQIAALARTDILRSEA
                     PLDFVHPLVREAIYRELPTGERELQHARAARVLLDTGAAPDHVATQILMAPPRGEEWV
                     VDLLEEAARAAIRRGAADGAVAYLRRALEEPPPTERRPLLLYELGLVEAGTNGPDALV
                     HLREAYDTLDDPRVRVEVAFWVTRLLMFTAQPDAAIEFAVQARADAPGEEFEDARRAL
                     EALELTAIYFGGGDPAQLDRMEANRGRPPSAEPGAKMLAATAALDWSVRDGSMEECTA
                     LAREALADGVLIEAENGHFTVTGAVPLVLAESDEAMVSYEAQLVDAHRRGSLFAVLAV
                     NLWRGWALLLRGELRDAEASLREAHAQTLLYGLREGIGITTTLGFLAQALVEQGRLED
                     ARTDLERVARPTAPSFAGDHWRRGELELLLAEGRHDEALAAAEEYRRQLQRTENPAIA
                     PWRTLTALALDGLGRTEEAIALAEEELAPARRWGAPGTVGRTLRILGTLQREAGLPRL
                     EEAVAVLEHSNARAERARALAALGAAIRRDRRPTEAREPLRRALELADAVGAQGLVAQ
                     VRTELAAAGAQPRTTALSGVDALTASERRVAGLAADGQTNRDIAQALYVTPKTVEVHL
                     SSAYRKLGIRSRRDLVGAMGT"
     gene            17083..17844
                     /locus_tag="VF517_10295"
     CDS             17083..17844
                     /locus_tag="VF517_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053373.1"
                     /translation="MNSILETQETILTKALTLAIAVCCAVAVAPATAGAAVKTKTAKF
                     WVEVNGTQVTDWEEKQHDTYFDCQGQRWSRGKGSEVIDFRTKKIRALITYRNGHAQVK
                     YGTWDRFAQGDYYMRGKGSVERIGEKVFGIEPDERCWDGGPTEQSGPYDCKRIPLTYD
                     VDLDWHDSMLSAEASPVVTTNARYEKCPIVTPDGVLGGVWTVVQSEPFPYRDLFDPKF
                     KYHEVLGGKDFTYDDKRFTTAHTKVRWTARFRRVK"
BASE COUNT         2655 a         6557 c         6378 g         2402 t
ORIGIN      
        1 tccaccccga gcccgcgcgc gtcgccctga taggccaggg aagagtcgag gaagcggtcc
       61 gacaccacca ctcggtccgc ctggagagcc gggcggatca cgtcggccac gagctgcgcc
      121 cgcgcggcgg cgaacagcag cgtctccgcc cgcgcgtcca gcggaatcga tcccttcagg
      181 acctcgcgca cgcgctcgcc gagctcggtc ccgcccggct cacggacggc cagcgcgcgc
      241 tcggcgccga gcgccgcgac cagcatccgg gcctgcgtcg gcttgccgga gcggtcgatt
      301 ccctcgaacg tgacgaacag accgggcacg cgccgaagat acccgccgtg caggcgtctg
      361 ccgcccggcg gcgtagattg caggcggtgc gtgccctagt gacaggcgga gccggcttca
      421 tcggctcgaa cgtggtggac ggcctcctcg cccagggcga cgaggtcacc gtcgtcgatg
      481 acctctccac cggcaagcgc gagaacctcg ggcaggcgct cgggaacggc gcccagctgg
      541 tcgaggccga catccgcgac gccgaggcgc tgaaggacat attcgcgcag gcgaggcccg
      601 aggtggtctt ccacctcgcc gcgcagatag acgtgcgcaa gtccgccgac gacccggcct
      661 tcgacgcgcg cgtgaacgtc gagggcacca tcaacgtgct tcaggcggcc cgcgcgtccg
      721 gcgcccgccg cctcgtcaac acgagcaccg gcggcgcgat ctacggcgag gcgcaggtga
      781 tccccgcgcc cgagtctcac ccgaacgcgc ccgaggcgcc ctatggccag tcgaagcacg
      841 ccgccgaggg ctactgcgag ctgttccgcc ggctcttcgg cctgagcacg gtgtcgctgc
      901 gctacggcaa cgtctacggc ccgcgccagg acccgctcgg cgaggccggc gtggtcgcga
      961 tcttctgcgg caagatccgc tccggcgagc gcccggtcgt cttcggcgac ggccttcaga
     1021 cgcgcgacta cgtgcacgtg agcgacgtcg tcgcggcgaa cctgacggcg gctggcagcg
     1081 actcctacgg cccgatcaac atcggcacgg gggtcgaggc gagcgtgctc gacctggtcg
     1141 aggccctggc cccgcacgcg agctccgact tcacgcccga ccaccagccc gagcgaccgg
     1201 gtgaggtgcg ccacatcgcc ctcgacgcct cgcgcgcccg cgctgagctc ggctgggagc
     1261 cgcggatggc gctcaccgag ggcatcgagc agacgctcgc gagcgtcggc tagcgcaacg
     1321 ccccggccga tagcgtgttc gggcggtggt gacggcggcg atcatctgcg cggcggttgg
     1381 gctggtcctg ctggggctcg gcgtggcctg gctcagcggc gcgatcgggg ggtccggcga
     1441 gggcgccgcg accgactggc gcgagcgcac cgcggacacc gcggctgact tccgcgactg
     1501 gctgcgcacc ggtcgctgaa gcgctcgccg ccgggcggcg gatcttcccg ctcgttgcgg
     1561 tttttcggcg gccaaaccgg ctgcaaatct ctagaaagca gctcgtgaaa acccggattt
     1621 cgggtagcga tttccgtccg accccctgag caacatgctg atgtggccac tttccgtggc
     1681 ccctgagtgc tgcgccgtaa gtcgaaggtc cggcggctcg ggaacagagc tatgtagtga
     1741 aaggaaggag cgaccatgct ggcgctcctg agacaggcac cgggcgaaga ggctttggtg
     1801 tctggaaggt tcgccctagt ggatcactac ggggtgccac tgtgcctcac tgtggcggtc
     1861 gaaaagggtg aacgactgcc gtacgcggca gcgactgaag acggcccgac gtggttcctc
     1921 ttggtcgatg tgttcgccaa gcagacccaa gcggcctagc agtgtgcggg tgtcgttgcg
     1981 acttgagccc tcgaaagcag agtcgcggcg gcacccgtcc ttttggggag cggggggaac
     2041 actaagaggc ttctgcccgc gagcgtgcat ccctggttgc tgttgccgat ctggccctgc
     2101 caagacctct tgatagtgct tcccatgccg gacagaacac ttttcccgga cgttgcgggg
     2161 agtaacgacg acccgctccg ccctgccaat cggttgatgc cgatcttccc gcttgttgcg
     2221 actttcgccc ggtcaaagct gctgctaatc cctagaattc gttgacgtca ccgttactga
     2281 cttttttctc tcgcaggccg cttttccgac cgacgccatt ggcagcatgt ccttcccctc
     2341 cgcgcaaccg gcgcggcccg ggaagaccgc caatacgtag ggagaagaga tgccccagtc
     2401 gcccagctca gccgccgaca tcaccgcccg ccgcaccggt cagggtgtga tggtcgagcg
     2461 tcgctacacg gagccgggcg tgcatccctt cgagcagatc gagtgggaga tccgcgacgc
     2521 cgtcatcggg aacccggaga agcccgcctt cgagcagctc ggggtcgagt tcccgaagag
     2581 ctggtcgcag aacgcgacca acatcgtcgc gcagaagtac ttccgcgggc gcctcgggtc
     2641 ggacgagcgc gagagctcgg tcaagcagat gatcggccgc gtcgcgggca cgatcgccgg
     2701 ctggggccgc gagctcggct acttcgcgac cgacgaggac ggcgacgcgt tcgaggctga
     2761 gctcacctac atccttctcc accagcaggc ggcgttcaac tcgccggtct ggttcaacgt
     2821 cggattcgag gagaagccgc agtgctcggc ctgcttcatc ctcagcgtcg aggacacgat
     2881 ggagtcgatc ctcggctgga acaccaagga gggcatgatc ttccgcggcg gctccggctc
     2941 cgggatcaac ctctcgaaca tccgctcgtc gaaggagtac ctctcgaagg gtggcctcgc
     3001 cagcgggccc gtctcgttca tgcggggcgc ggactcctgg gccggcacga tcaagtcggg
     3061 cggcaagacg cgtcgcgcgg ccaagatggt cgtgctcgac atcgaccacc cggacatcgt
     3121 cgacttcatc tggtgcaagg cgtccgagga ggagaaggcc gccgcgctgc gcgacgccgg
     3181 cttcgacatg tcgatcgacg gcgagggctt cacctcgatc cagtaccaga acgccaacaa
     3241 ctcggtccgg gtcaccgacg agttcatgca cgccgtcgag gcgggctccg actggcacac
     3301 catcgcgcgc gtgagccgcg agccggtcga cacctacgac gcgcgcgacc tgatgcgcca
     3361 gatcgccgac gccgcgtggc gctgcgccga tcccggcgtc cagtacgaca ccacgatcaa
     3421 cgactggcac acctgcccgg ccagcgggcg gatcaacgcg tcgaacccgt gctcggagta
     3481 catgcacgtg gacgactcgg cctgcaacct cgcgtctatc aacctcatga agttccgccg
     3541 ccccgacggc agcttcgacg cgtcggcctt cgagcacgtc gtcgacgtga tgatcctcgc
     3601 gcaggagatc gtcgtcgggc cgtcgagcta cccgaccgag ggcatcggcg agaacgcccg
     3661 cgcattccgc cagctcggcc tcggctacgc caacctcggc gcgctgctga tggccgacgg
     3721 gctcccgtac gactccgacg agggacgctc ctacgccgcc gcgatcaccg cgctgatgac
     3781 cgggcgcgcc taccgcaagt cggccgaggt ggccggcgcg atcgggccgt acgagcgcta
     3841 ccccgagaac cgcgaggcgc acaaccacgt gatggagaag caccgcgcgg cgtcgtacga
     3901 gctctccgac accgagtgcc gcgacgcgga gctgctcgcc gcggcgcgcc gctcctggga
     3961 cgaggctgtg gcgctcgggc tcatgcacgg ctaccgcaac gcgcaggcca ccgtgctcgc
     4021 gcccaccggc acgatctcgt tcctcatgga ctgcgacacg accggcgtcg agccggactt
     4081 ctcgctcgtg aagttcaagg agctggtcgg cggcggtcag atgacgatcg tcaacaagac
     4141 ggtcccgctg gcgctcgagc gactcgggta cggcgaggcc gaggtcgacc agattggcgc
     4201 gtggatccag gagcacggca cgatcctcgg tgcccccggc ctcaaggacg agcacctgcc
     4261 ggtgttcgac gtggccgtcg gacagcgcgc gatctcgcac atgggccaca tccagatgat
     4321 gggcgcggtg cagccgttca tctcgggcgc gatctcgaag acggtgaaca tgccggccga
     4381 gaccacggtc gaggacatcg ccgatgcgta cgtgcaggcg tggaagctcg gcgtgaaggc
     4441 gctggcgatc taccgcgacg ggtcgaagac ggcgcaggcg ctgcgcacgg acgcgcacaa
     4501 gggcaaggac gcacccgcga ccgccgaggt ggtcgaggag accccgcggc cagcccggcg
     4561 caagatgccg aaggagcggc agtcgctcac gcacaagttc tcgatcgccg gccacgaggg
     4621 ctacatcacg gcgggcatgt acgaggacgg gacggtcggc gagatcttcc tcaccgacat
     4681 cggcaaggag ggctcgacgc tgcgcggcat gatgaacgcg ttcgcgacct cggtctcgat
     4741 ctcgctccag tacggcgtgc cgctcgagac gctcgtgcgc aagttcagct acatgcgctt
     4801 cgacccggag ggcgcgaccg gcaacccgga gatcccgttc gccaagtcga tgcccgacta
     4861 catcatgcgc tggctcgcgt cgcggttcct cgacgtcgac tgccaggagg agctcgggat
     4921 catgacccac gaggtccgcg cgcggaaggc ggcgcaggaa gccctgatgc gcggggacac
     4981 ggcggcgcca gtggtagagg tcgagccgac gaacagcggc ggcaacgggt cgggcaacgg
     5041 gcacgcgaac ggcgggacca acggcggcaa cggctcgccg aaggcgtcgg ccggcaacgg
     5101 cggcacgaag cccgctgccg cggcgttcac ggacgagccg ccggtcatcc ccgcgcggct
     5161 acagggcctg gacctcggcc cggcctgcgg ccagtgcggc gggatgatgc agcggacggg
     5221 cagttgctac acctgctcgt cctgcggcaa caacaccggc tgcggataac cggcgggctg
     5281 caaaccttcg ctctccggcg tcctcgggtc tcgccgtgct agtcgcacgg cttcgaccct
     5341 gcgtccttgg atagctcggt tttcgcaccg gttaccgcgc cgtgccagct cggtgccacc
     5401 aacgggccgc ctgagggcgg cccgttgctt tttctggctt ttgcacacag ggtgtgcgca
     5461 tgaactggat cgtgatcggg gtcgtgctcc tgctggcggc gctgttcgag gtatgggtcg
     5521 agctgatcgc gcagaaccat ccgtggcggg tcgcgatcgt cggcggggtc gtgttcctcc
     5581 tcgtcggggc gatcctgttc ctctggccga gcggaccgct gatcacactc acgttgctgc
     5641 tcgccggcgc ctgggcctgg gcgctcgtcg cgggactcga cgcccgcgac cacgagctgg
     5701 gttactactg ccgctacggc gcgcagaccc agaccgagct cgaccgctgc atgtcgagca
     5761 tcaacaccga cgagatcgag gagctcgaca cgccggcggc gcgcttcgcg gtcggcgtaa
     5821 ggaccgagtg cgggcccggc tccggccgct tctgccgcga ggccgcgaag gacgccgcga
     5881 tcgagtacgg cggctgaccg ccgtgaggag cccgcggcgc taaccgccgt gggcgccggg
     5941 ccgcaagccc ccgccacgta ggctccccct cgtgtcagag cgcctcgtcg acatcagcca
     6001 cccgatcgcg gacgggacga tcacgtaccc gggcctgccc gcgcccgaga tctcggagca
     6061 cctcacccgc gacgaggccg aggagcacta cgccccgggc gtcaggttcc acatcggacg
     6121 catatgcatg atcgccaaca ccggcacgta cgtggactcg ccgttccacc gcttcgagga
     6181 cggcgtcgac ctcgccggcc tcgagctgcg ccggctcgcg gggcggcccg ggatcgtggt
     6241 ggacccgccg ctcgacgacg ggcgcgcgat cgaggccgtc gccttccacg acctcgacct
     6301 cgcggaccgg gcggtgctcg tccggaccgg ctgggaccga cactggggca cgcccgagta
     6361 cggcgtcggc tacccgttcc tgaccgccgc ggcgggcgag ttcctgctcg aggcgggcgc
     6421 ggcgatcgtc ggcatcgact cgctcaacgt cgacgacgac accgacccgc agcgcccggt
     6481 ccatacgacc cttctgggcg cgggcatccc gatctgcgag cacctctgca acctgacggc
     6541 cctccccacc tggggcttcc gcttccacgc cgcccccgtg aaggtcgagg gcctgggcac
     6601 cttcccggtg cgcgcctacg cggagctcga ctgagctccc tacagcggcg gtagtgaagt
     6661 agcgtctgtc cgccatccgg ccggagcccg cgaatgcctg acgcctcgga cgaagccaat
     6721 gacatagtcg acgaagtgct cgatgccctg atgcccaacg ccaactactg ggcaccgcgg
     6781 accatcgacg acggggctgc tcctgcccca gagcaggtgc gcggaaaagc gggaggcgat
     6841 ggcgaggacc gagttcgcta cttcgccggg ccggaagctc tagaggtcct ggcgtggatc
     6901 gcaacgtcgg tggtcgttcc tttgatcgtc ggagtcgcat cgaaccgcat cgacgcgcgt
     6961 ctccaggccg ggagcgtgcc ggaaccaccc cggccgaggg gccagactga agcgatggcg
     7021 gcagtggcgg acgccattgc gcgccgccaa cctcacaccg acgatcgcga cagcgtcgag
     7081 ttggcggagg agcacgtccg tctgcttctg atcgattacg gctggccggc gaaggaagcg
     7141 ctcagcgacg ccaccgagat cgtcgggcgc gtaagccgcc gcctccgttc gcagtgaagc
     7201 caacgtccgc cgacagcgcg ccgcgagagc tttcgcggcg gcgagatcac gtactcgtaa
     7261 agagcgctgc cggttcgcaa cacgtcagga acgagttcct gaggcgacgg ctgcaggctg
     7321 tcgcggccgg cctgcccgaa acccccgcgc gagatccgaa agatgacgcc aagtcgcaag
     7381 cgccggccct gaagcgcgtc gagggatctt caaccggtgc gcgaatcttc gccccgcaat
     7441 tcggcaacga actttcggcg agcgaccacg ccgcgttgtg ggaggacttc gtcgcgcggt
     7501 ggttcggcga cgacaacgag gacgccacgg atgagctgat gcgggcagtc ggcgacgcga
     7561 tcatgtcccg cgaccgcgac ctcgttctgg aaatcctcga cgcactctgg cgcgacaggt
     7621 ggatcctcga tcagacgctt gccgcgtggc tccgcgagtg gtcgttcagc ccccagttcg
     7681 gcgacttccg cgcacgcgtg cgcgatgcac ctgaggagct tgatcgcgcg ttcgacgagt
     7741 tgagaggtgc gatcgccgcg gcccaggacg cgctcgatct ggattccttc ctcgcgcgac
     7801 tccacaccct gttgccgatc gcgcattacg cgcgccgcgt cgctgacgtc gcggaccacc
     7861 cagacgcagc caagcactcg cgattctccg aggaggcgtg ggtcgacgcc accggcgtga
     7921 taacccgagc tgtcttggga gccgccgaaa acaagccggg tgacatgtgg gtgcgatcgc
     7981 gcgtggatgc cctgacgcca gtccggccgg aacctcgtgg tctgcgccgg tcactccgtt
     8041 actacgtggc gcgcttggaa tttccacgcg tctgggtcga cgctgtactc gagtggcaag
     8101 cagggggcgg ggccccgccc tcgtggttga cgacctacga cgacctgacg ctcgcgtcgc
     8161 tggcggttac cgcgtttccg gggggatcga gcgcggcgtt ccgccgcccg cccgccccgt
     8221 caggttgagc actccgcgcg ggccccggcg ccccctcccc cggctccgcg aacttcgcca
     8281 gccgccggaa cgccttcacc cgctcgctgc gctccacccc acgcgcgccg ttcagcgcgc
     8341 tctccaggat cgacacggtc cggtcgtagg tcgcccgatc gaccgggaac ggcgtgccgt
     8401 ccttgccgcc gtgggcgaac gcgtaccgcg ccgggtcccg cgtgctggca cgcgtcccgt
     8461 agaccagctc gctcacgagc gccagcgagc gcagcgtctt cggccccacc ccctggatcc
     8521 cgagcagctc ctcgaacgag ctcggcgcag cggcgtaggt cttcaacagg atccggtcga
     8581 tgtaggccgg attcacgtcg tcgagcttca gctcgtgccg gcgcggcatg accagctcgg
     8641 aggcccgcgc gatcgcgtcg agcgaccgct tcgggtcgga ggcggcgagc tccacggcgg
     8701 ccgcgcgcgc gtcggcgctc tccgcggcca cgaagttcac gagcacgtcc ccgcgcgcgt
     8761 cgtcgcacac cgccgcgtgc ggctcctcga cgaagctgtc gacgctctcg ccgagccagt
     8821 ggaagcgccg cgccgacttg gtctcgtcgg acatgccctg ctggatcacc gcccagctgc
     8881 cgtcggccag gaacatgaac gagtggtggt agagctgatg accgctctga acggccgtgt
     8941 tgtcgacctt cgccgacacc ttgctcgccc gcacgagcgg cgccggatcg cgccccagtc
     9001 gctcgcagaa gcgctcgatc tccgacggcg tgcggcgcga tgtcgccccc ttcccgccgg
     9061 ccacgaagaa gccgagctcg tgctcgagcc cgcggacccc ctccttcaac gctccggtgg
     9121 ccgtggtggt cacccccgac gagtgccagt cgaagccaag aacgcacgcg aacgcctgga
     9181 accagtgcgg gtccgacagc cgccgcagga tctccgcggt accgagctcg gacgccatga
     9241 acaccacgat ctcccgcgag aggcgcgtca tccgctggaa cagccaggcg ggggcgcgcc
     9301 ccccgtggag cgggaggtcg gcggttccgg tgcggcggcg tgaggccacg acaagcaggg
     9361 taggttcagc gtcggtggcc acgatcgacg acttgctgga gcgcgcgcgg gaggacctcc
     9421 agcgcgttac cccggaggag gccgcggccg ccgtggccga acgcgacgcg ctgctgctcg
     9481 acatccgcgc cgactcgcag cgcgccgccg acggcaccgt cccgcaggcg cgcttcgtgg
     9541 cacgcaacgt gctcgagtgg cggctggacc ccgcctgcga gcaccgcgat ccggacctcg
     9601 ccagctacga gcgcccggtg atcctcatgt gcaacgaggg ctaccagtcg agcctcgcgg
     9661 cggcgaccct gcgggagctc ggcgtggacg ccaccgacat ggtcggcggc ttccaggcct
     9721 ggcgcgacgc cgggctgccg accgccacgg acgagccgga tacggcctag ttaggcgttc
     9781 tcgtcgcgct cgacgcgcgg cgactcgacg ccggcccgca ccgcctcgag ctgcgcggcg
     9841 aacgccagcc cgccgaacag agccaccgac gtcaggaacg cccacagcag caccccgatc
     9901 gtccccgcga ggggcccgta ggtctgcccg aacgactccg ccacgtcgat gtagagcgcc
     9961 agcacgccca tgaacacgag ccacagcagc gccgacgcgc cggctccgaa ggcaagccac
    10021 gacggctcgg gctggtgccg gcgcggcgag tacacgaaca ggatcgacac cgacgcgacc
    10081 acgaacgcaa gtccgagcgg ccagcgcacg atcgcccaga aggagtcgaa gccgtcgctc
    10141 cacccggccg cgtcgcgtat ggcggacccg ccgacgagaa gcaccaggga gagcaccgcc
    10201 agcaccccgg ccgacagggc gatcacgagg gccacgccgt acttgcgcgg gaacgggcgg
    10261 tcccgctcgg tcccgtagat ccggttggcc ccgcgctcga tctgcgccat cgcctgcgtg
    10321 cccgcgatca gagccgccac gaacccgccg accagcgccg tctccccgga ctcgcgcgcg
    10381 gcggcgccgc tgccctgccg cagcgcgtcg gtgaggatct cgcccgccgc gcccggtgcc
    10441 agatccgtga cgagagcctc caccacccgg cggaacgagt cctggtcgag cgccgccgcg
    10501 aggccgacca cggctatcag ggcgggtacg agcgtgagcg tcaactgaaa ggcaagcgcg
    10561 cgcgagtggc tgaacgcgtc ggccgcgcga aagcgcgcga tcgtgtcgac caggagtttc
    10621 ctgcgcccgg tgcgcagcag cgtctcgagc gcctcgtcgc cctcgagctc gccacgggtg
    10681 cgcggaacgg cggtcgcggt cgacacggtc atgcatcttg ctgatgtctt ccattccggg
    10741 tcgccccgcc cacccgaccc gccgcacgtt caggcgcgca gcgcggcggg gtactcggga
    10801 cgcatgacca agcgacaggt ggaggatccg gtcgggcgcc agcgactcgc gttcgagctg
    10861 ggcacgaacg acgacggcac cgagaccgtg ctcgtcgagt gctgggtcga cccggggggc
    10921 ggtgtcccgc cgcacatcca cccgcaccag accgaggtct tcgaggtggt cgagggcgag
    10981 atgaccttca ccgccggccg cacgaagcag gtcgcgcgcg cgggcgagac ggtcacggtc
    11041 ccgcccggca cccgccacgc ctacgagaac aggagcgcga gcagcgcgta catgcgctgc
    11101 gtcgccacgc cggcggccgg cctccaggac ttcctcgaga ccaccgcgca gctctcccgc
    11161 cagggccaca tcatgcggat cggcccgctg cgagggccgg gcagcctgcg cggcctgccg
    11221 aagctcgcgg ccatgctcga gcgccatcgg gagaacacgg tgatcctgat gccgccgccg
    11281 ctggtccagc gcctcgtgct cttcccgctc gcgcggttcg cggatcgctc gcggcgcggt
    11341 tgaacgaccc ttaccatggc gcgggatgga gcgcagcggc actggctcgc cgatcgctga
    11401 cctcgccggc gacaagaccc tgctcgaacg cgagcacgag ctcgcgtccc tcgatgtcct
    11461 gatcgcggcc gccgccgagc gtcaggcgcg ggttgcggtc gtcgagggcc gcgccggaat
    11521 cggcaagagc cgcctgatcg cggccgcccg cgagcacgcc gcggcgagcg ccacgcgggt
    11581 gctatccgcc cgcggcacgg agctggagcg cgagttcccg tacggcgtcg tccgccagct
    11641 cttcgagccg ctgcgctccg acccggacga gtgggagcgg ctgctcgagg gcgccgcagc
    11701 ctccgcccgc ccgctgttcg aggcgccgag cgccgcggac ggcgacggcc aggtgcgcga
    11761 cgcctccttc gccgcgctcc acggcctcta ctggctcgtc gtgaacctca cagccgagac
    11821 cccgctgatg ctcgccgtcg acgacctcca ctggtgcgac cgcccgtcgc tgcgtttcct
    11881 cgcctacctc gcgccgcggc tcgaaggcct tccgctcctg ctcgtgaccg gactgcgcac
    11941 gggcgaggcg cccaccgacc cggtcctgct cgacgacgtg gtgaacggac cggccgccac
    12001 cgccgtccat cccggcccgc tcagcgcgga ggccgtcacc gcgatgatcg gcgcgcgcct
    12061 cggcccggag gtcgacgagc gcttcgccgc cgcctgcctg gagtcgaccg ccggcaaccc
    12121 gctgctgctc ggccagctca tcagctcgct ggcggcggag ggcgtggtcc cggacgccgg
    12181 caacgcggag aaggtgcgcg cgatcggacc gcgcgcggtc tcgcgcaccg tgctcctgcg
    12241 cctctcgcgc ctctcccccg gcgcgatggc cgtggcacgt gccgtggcgg tgctgggcga
    12301 gaacgccgag ctggccgcca tcgccgcgct cgcggagctg gacgagaccg ccgtggccga
    12361 ggcgacggca tcgctcgccc gcgccgacat cctccgccac gacctccagc tcggcttcgt
    12421 ccacccgctc gtccacgacg cggtctacca ggacctcccg cccggcgagc gcgagctcca
    12481 gcacgcgcgc gccgcccgcg tgctgctcga cggcggagcc gcccccgacc aggtcgcgac
    12541 ccatctcctc atgaccccgc ggcgcggcga ggagtgggtg gtcgacttgc tggtgggcgc
    12601 ggcgcggtcg gccgtgcgca agggcgccac cgacagcgcg atcgcctacc tgcgccgcgc
    12661 gctcgaggaa ccgccgccgg aggagcgccg ggccgacctg gtctacgagc tgggcttcac
    12721 ggagtcgaac gccagcgccc ccgacgccgt cgggcacctg cgtcaggcct acgagctgtg
    12781 ctccgacccg cgccgccgtg ccgactcggc gttctggctc tcgcggctgc tgatgctgct
    12841 cggcgacccc gaccaggcgg cggcgatcgc cgcggaagcc atcgcgcaga ccccggccga
    12901 gctcgaggac gagcgccagg cgctccaggc ccagcagctc accgtcgcgt tctggggcgg
    12961 cggggacctg agcgcgctcg acgaccccga ctcgccgccg ccacgcgtca gcggcagcgg
    13021 cccgggcgcg aagatgctcc tcgcccacac cgcggtggcg tgggtcgtca acaaccgtcc
    13081 cgcggccgag acctgcgcga tcgcgctcga ctcgctcgcc ggcggtgtcc tgatcgaggc
    13141 cgagaacggc tacttcaccg tgggggccgc cgtgccgctc gtgctcgccg agcgccgcga
    13201 gggcacggag gtgtgggacg cgtgcttcgc ggacgcgcac cggcgcgggt cgctcttcgg
    13261 cgcgatgggc gctcacatgt ggcacgggtg gacgctgatc aggctcggca acctgcccga
    13321 ggccgaggcg tccctgcgca cggctctcac cgagatggag atgtggggca acccgcgggt
    13381 ggagtacacg ctcgggttcc tggcctgggc gctgatcgag cgcggccgcc tcgacgaggc
    13441 gcgcaccgcg ctcgagcgcg tgccgctcga gccggtgccg acctggggcg ggacgcactg
    13501 gaggcgctcg cgcgtggagc tgctgctcgc cgagggccgc cccgaggagg cgctcgcggc
    13561 gctcgacgag gtgtccggct cggtgttcgc ccgcaacccg gcgctgttcc catggcgcac
    13621 gcaccgcgcc ctggcgctcg acgcgctcgg tcgcaccgac gaggcgatcg cgctggcgga
    13681 ggaggaactc gccccggcgc ggcagtgggg cgcgcccgcc accgtcgccc gcacactgcg
    13741 catcctcggc accctccagc gcgacgaggg cctgccgcgg ctcgaggagg cggccgcgct
    13801 gctcgagcgg tcgaccgacc ggcttgagca cgcgaaggcg ctcaccgcgc tcgggtcgac
    13861 gatccggcgc gcacgtcagc cgaccgaggc gcgcgagccc ctgcgccgcg cgctcgagct
    13921 ggccgcggcg tgcggggccg acggcctcgt ggagcacgtg cgcgcggagc tggcggccgc
    13981 cggagcgcag ccgcgcacca ccgcgctcag cggcgtcgag gcgctcaccg ccagcgagcg
    14041 gcgcgtggcc ggcctggccg ccgagggcca gaccaaccgc gacatcgcgc aggttctcta
    14101 cgtcacgccg aagaccgtcg aggtccacct ctcgaacgcg taccgcaagc tcgggatccg
    14161 ctcccgccgt gagctgagcg gcgcgatggc cacgtgagcg acccgctgct cgaacgcgag
    14221 cgcgagcgcg cgacgctgca ccggctgctc gacgcggccg ccgggcagca ggccgggctg
    14281 gcgctcgtcg agggccgcgc ggggatcggc aagagccgcc tgctcgccga cctgcgcgag
    14341 cacgccgccg ccgagggcat ccgcgtggtc tccgcacgca gcaccgagct cgagcagggc
    14401 ttcccctatg gcgtggtccg ccagctcttc gagccgctgc gggcgaccga tccggagcgc
    14461 tgggacgcgt ggctcgcggg gtcggccgat ccggcgcgga tcgtcttcga gacgccggtg
    14521 gcgggcccgg acgccgacga gctgcgcgac gcgacgttcg ccaccctcca cggcctctac
    14581 tggctgaccg tgaacctgac ggccgacggc ccgctgctgc tcgccgtcga cgacctgcac
    14641 tggtgcgacc gcgcctcgct gcgcttcctc gcctacctcg ctccgcggct cgaggggctg
    14701 ccggtgctcg tggccacggg cctgcggacc ggggagccgg gcaccgaccc ggcgctgctc
    14761 gccgagatcg tgagcagccc ggccgccgtg gccgtgcagc cggcgccgct cgggtccgag
    14821 agcgtgggcg agctggtcct cgcgcgcctg ggggccgagg ccgacgagcg attcacggcc
    14881 gcctgcctcg acgctaccgg cggcaacccg ctgctcctga gccagctcat cagctcgctg
    14941 gaggccgagg gcgtgaagcc ggacgcggag cacgtggacg tggtgcgcca gatcggcccg
    15001 cgcgccgtgt cgcgcacggt cctgctgcgg ctctcgcggc tgcccgccgc ggccggtgcc
    15061 gtggcccgcg cggtggcggt gctgggcgag agcgccgagc tgcggacggt cgcggcccac
    15121 gccgagctcg accaggcgga ggcggccgag cagatcgcgg cgctcgcgcg caccgacatc
    15181 ctccgctcgg aggcgccgct cgacttcgtt cacccgctcg tccgcgaggc gatctaccgc
    15241 gagctgccga ccggcgagcg cgagctccag cacgcacgcg cggcgcgggt gctgctcgac
    15301 acgggcgccg cgccggatca cgtcgcgacg cagatcctga tggcgccacc gcgcggcgag
    15361 gagtgggtcg tggacctgct cgaggaggcg gcgcgcgccg ccatccgcag gggcgcggcc
    15421 gacggcgccg tggcctacct gcgccgcgcg ctcgaggagc cgccgccgac cgagcgccgc
    15481 ccgctgctgc tgtacgagct cgggctggtc gaggccggga ccaacgggcc cgacgccctc
    15541 gtccacctgc gcgaggcata cgacacgctc gacgacccgc gcgtgcgggt ggaggtggcg
    15601 ttctgggtca cccggctgct gatgttcacg gctcagcccg acgccgcgat cgagttcgcg
    15661 gtccaggccc gcgccgacgc gcccggcgag gagttcgagg acgcacggcg cgcgctggag
    15721 gcgctcgagc tgacggcgat ctacttcggg ggcggcgacc ccgcgcagct cgaccgtatg
    15781 gaggccaacc ggggaaggcc ccccagcgcg gagcccggcg ccaagatgct cgcggccacc
    15841 gccgcgctcg actggtcggt tcgcgacggg tcgatggagg agtgcaccgc gctcgcgcgc
    15901 gaggccctcg ccgacggcgt cctgatcgag gccgagaacg ggcacttcac cgtcaccggg
    15961 gccgtgccgc tggtgctcgc cgagagcgac gaggcgatgg tgagctacga ggcccagctc
    16021 gtcgacgccc accgccgcgg ttccctgttc gcggtactgg cggtgaacct gtggcgaggc
    16081 tgggcgctgc tcctgcgcgg tgagctgcgt gacgcggagg cgtcgctgcg cgaggcgcac
    16141 gcgcagaccc tgctctacgg gctgcgcgag gggatcggga tcacgaccac gctcgggttc
    16201 ctcgcccagg cgctggtaga gcagggccgg ctcgaggacg cgcggacgga tctcgaacgc
    16261 gtggcgcgac ccaccgcgcc gtcgttcgcg ggcgaccatt ggcgccgcgg cgagctcgag
    16321 ctgctgctcg cggagggccg ccacgacgag gcgctcgccg ccgccgagga gtaccggcgc
    16381 cagctccagc gcaccgagaa cccggcgatc gcgccgtggc ggacgctcac ggcgctcgcg
    16441 ctggacgggc tcggccgcac cgaggaggcg atcgcgctgg ccgaggagga gctcgcgccg
    16501 gcgcggcgct ggggcgcgcc cggcacggtg gggcgcacgc tgcggatcct cggcacgctc
    16561 cagcgcgaag cggggctgcc gcgcctcgag gaggccgtgg cggtgctcga acactcgaac
    16621 gcgcgggccg agcgggcgcg tgcgctggcg gcgctggggg cggcgatccg gcgcgaccgg
    16681 cgcccgaccg aagcgcgcga gccgctgcgc cgcgcgctcg agctggcgga cgccgtgggg
    16741 gcgcagggcc tcgtcgccca ggtgcgcacc gagctcgcgg ccgcgggcgc gcagccgcgg
    16801 accacggccc tcagcggggt cgacgcgctc acggcgtccg agcgccgcgt ggcgggcctg
    16861 gcggcggacg gtcagaccaa ccgcgacatc gcccaggcgc tctacgtgac acccaagacc
    16921 gtcgaggtcc acctctcgag cgcgtaccgc aagctcggca tccgctcccg gcgcgacctg
    16981 gtcggcgcca tgggcaccta gggggttctc ccccaaagac tcgggtgcct ccttgggggc
    17041 gtcctcgatg tgcgcaggct cgcgcggctc gaagctgagc ccatgaacag catcctcgag
    17101 acgcaggaga cgattttgac gaaggcactc acactcgcaa tcgccgtgtg ctgcgccgtg
    17161 gcggtggccc ctgccacggc cggcgcggcc gtcaagacca agaccgcgaa gttctgggtc
    17221 gaggtcaacg gcacccaggt cacggactgg gaggagaagc agcacgacac gtacttcgac
    17281 tgccagggtc agcgctggtc gcgcggcaag ggctccgagg tgatcgactt ccggacgaag
    17341 aagatccgcg cgctgatcac gtacaggaat ggtcacgcgc aggtgaagta cggcacctgg
    17401 gaccggttcg cgcagggcga ctactacatg cgcggcaagg gcagcgtcga gcggatcggc
    17461 gagaaggtct tcgggatcga gcccgacgag cgctgctggg acggcgggcc gaccgagcag
    17521 agcgggccgt acgactgcaa gcggatcccg ttgacctacg acgtcgacct cgactggcac
    17581 gactccatgc tctcggcgga ggcctcgccg gtggtcacga ccaacgcgcg ctacgagaag
    17641 tgcccgatcg tgacgcccga cggcgtgctc ggcggcgtct ggacggtcgt gcagagcgag
    17701 ccgttcccgt acagggacct gttcgacccg aagttcaagt accacgaggt actcggaggg
    17761 aaggacttca cctacgacga caagcgcttc accaccgcgc acacgaaggt gcgctggacc
    17821 gcgcgcttcc gccgggtgaa gtaggccgca gcggggcgag ggccggcggc gcgtgtgccg
    17881 ccggccctcg tcgttcgccc gcgggcggga gcccggggct ccccgctgac gcccgggcgg
    17941 ggacccgggc tggacggcgc ccgccggagg gagaccggcg ggcgctcggg gt
//
LOCUS       DASVPL010000198        17833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_62803, whole genome shotgun sequence.
ACCESSION   DASVPL010000198 DASVPL010000000
VERSION     DASVPL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17833
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62803"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1952
                     /gene="aspS"
                     /locus_tag="VF517_10300"
     CDS             171..1952
                     /gene="aspS"
                     /locus_tag="VF517_10300"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX8053374.1"
                     /translation="MRSAPRENAYREAWTGELRSADVGRTVTVAGWVHRRRDHGGLIF
                     IDLRDRTGLLQIVFHPESAPEAHAAAHDLRAEDVISVGGRLTRREEGQVNPNLPTGEI
                     ELTVTELEQLADADTPPFQVDDEGTVAEELRLRYRYLDLRRDRMRQAIELRHRVVAAI
                     RDHLNAQDFLEIETPMLTRSTPEGARDYLVPSRVQHGSWYALPQSPQLFKQLFMVGGL
                     ERYFQIVRCFRDEDPRADRQPEFTQLDIEMSFVEEEDVFALIDPLLESLLGIVGIEVS
                     FPLDRMSYDEAILRYGTDRPDRRPGMEIHDLGEVFAGSEFKVFAGALESGGVVRGFRA
                     SGGEWPRRRFDELTERAKSLGAKGLVWAVVERAEDGSGLGWRSRVAKFLQADEMARAV
                     EALGAGEGDVLLLVADDAKVAARVLGDLRLEVAGPQEGHDLLWVVDFPMFEWNEGEGR
                     LDPLHHPFTSPGTDLDDDPAKWRSRAYDVIMDGWELGGGSIRISDIDVQERVFTAIGL
                     APEEARERFGFLLEAFRYGAPPHGGIAFGLDRIVAMLAGRESIRDVIAFPKTSTAQDL
                     LTGAPAPVDEAQLKDLGVALRKPPPGA"
     gene            1990..2146
                     /gene="ssrS"
                     /locus_tag="VF517_10305"
     ncRNA           1990..2146
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF517_10305"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2321..3850
                     /gene="mnmA"
                     /locus_tag="VF517_10310"
     CDS             2321..3850
                     /gene="mnmA"
                     /locus_tag="VF517_10310"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX8053375.1"
                     /translation="MEDHLAAPRGRGRLRDGAHAGSAGGAPCGDLIRIALEVRDGRVA
                     DAGFDASGCSAAQAAGSAVVELVRGRPVLDAARVAPATVSDELGGLSPERHHAAALAA
                     DALHRALAAAAADPAALLAPGVVGDPAATPASPLAGRTLVAMSGGVDSAVAAHLCRER
                     GDDVVAVTLELWADAANDGTRSCCSPQAVTHARALAHRMGLPHLTLDLSEAFRHDVVD
                     DFLAEHAAGTTPNPCVRCNGLVRFGAMLALAERVGAERLVTGHYARIARDEQGPLVRQ
                     AADPLKDQAYMLARLDPELLDRLEFPLGDLAKPETRAIARAAGLPVADRPDSQDLCFL
                     AGTTAQAFMGRHAGPAARPGEIVDERGRVLGTHDGQHRFTVGQRRGIGVSSREPLYVV
                     RKHAGSNRVVVGPRAALARRSIALSGATLHRDGAEVDRVKLRYRSDPLPCRVTAAAPA
                     GRHGRLELELGAEVHGVAPGQTACLMRRDAVVGWATIRPDEMPMPDSHSLPMSEVALA
                     P"
     gene            3840..4229
                     /locus_tag="VF517_10315"
     CDS             3840..4229
                     /locus_tag="VF517_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053376.1"
                     /translation="MPRDRIELTTPVGRAWEAVVRMVLGGIADRLDLGFDQLDDLQLA
                     VERLLAEGDPRDPVSLSFELTEGGVRARIGPLRDTALAEALQGPEPPPGVLTLPRILS
                     TVVDSYGIEEVADGHLIVRLEKLVRRQ"
     gene            4226..4966
                     /locus_tag="VF517_10320"
     CDS             4226..4966
                     /locus_tag="VF517_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX8053377.1"
                     /translation="MSTVQPVTDDRELLRRYHVDGDPSAREELVNRHLPLVRSLARRY
                     AGRGEALEDIEQVGAIGLIKAIDRYELSREVALTTYATPNVVGAIKRHVRDKGWAIRV
                     PRALQELNGKMGPTIERLTSKLGRSPSIAEIAAEFETSTEQVLEALEAGSAYAPLSLS
                     TGPSGDEELDPMETIGELDEEFDRTDDRTSLEPALAALPEREREILRMRFEDGLTQTQ
                     IAERIGISQMHVSRLIRRSLERMRNQLT"
     gene            4998..5957
                     /locus_tag="VF517_10325"
     CDS             4998..5957
                     /locus_tag="VF517_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8053378.1"
                     /translation="MTTAAAEIVPGPPPDIAARSPFELFWRRLRRDRLAFAALGFIVF
                     LIVVALLAPVIVDVTGSRPPNETGKRFLDPEFGTATGPSSENPFGVDTLGRDVLSRVL
                     YGARVSLQVALIATALSVLIGLLVGLVAGYYRGWVDAVLSRVIDVLLAFPILLLALGI
                     AAACSFGEGCLRGVVKPGLGVVIFVIAFVNWTYIARIVRGQVLQLREREFVEAARSLG
                     ASDARILFREILPNLTAPLLVYTTLAIPQNILFEAALSFLGVGVQPPDASWGKMIADA
                     TAVFDTAWWYMLFPGAALLLTVLSFNILGDGLQDALDPRVGGN"
     gene            6015..7739
                     /locus_tag="VF517_10330"
     CDS             6015..7739
                     /locus_tag="VF517_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8053379.1"
                     /translation="MKRMRKQWLMAAIVGVLALVAAGCGGGDDEAKQGGTITVLAVND
                     IISLDPGAQYYQYDYMVVSQPGQRSLYGWKAEETKPTPDLAASMPQVSDGGRTVTIKL
                     RKGVKFSPPVNREATSADVKYAMERLFLPSVGNGYASVYFNGIVGAEEFTEGKAKEIE
                     GIQAPDPQTLVLKLENPVGVIANANALTLPGTAPVPKDYASKYDKGETSTYGEHLVFT
                     GPYMVKNDGKGNITGFTPNKRLELVRNPNWDKTTDYRAAYVDRIVSLAGTDATVASRR
                     ILSGKGLVSGDFAAPPTPVLKQALSSRKDQLDIVDSHGNRFIALNTKVKPLDNVNVRK
                     AIAAVVDKEELRLTRGGPTLGTVASHFIPPGIPGHDEAGGEAGTGADFLSKPQGDVAL
                     AQEYLRKAGFASGRYEGPELLMVGDDDSPPKETGEALQAQLQKIGIKLNYRQVPHNTM
                     ISKFCGTPKAAVAICPNLGWGPDFFDAQSMVDPIFNGENIAPTNNSNYAQLDDPKLND
                     AMAKATMLTDPAARAKAYGELDRQISELAPVIPWLWDKQVNIRSKDVNGVINAFNAAY
                     DLNFTSVE"
     gene            7762..8724
                     /locus_tag="VF517_10335"
     CDS             7762..8724
                     /locus_tag="VF517_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8053380.1"
                     /translation="MIAYLLKRLAWVVALLFLVSAITFVIFYLMPSADPAQLRAGRQP
                     TPELVERIRHQLGLDQPWYEQFWRYMKDLVLHFDLGRSYQSNVPVREQLFDRLPATLS
                     LALGAAVIWIVAGVAVGTVSAIRRHTLLDRAAMTAALVAISAPPYWLGLVALYLFAND
                     IGRFHVFEGAASYAPLTEDPLQWASSLVLPWLVLAASFAAIYARLVRATLGEAMSEDY
                     IRTARAKGLRERRVVVRHGLRSAITPVVTAAGLDIGVLLGGAILVETVFNIPGIGLLA
                     YESILDADLPMIQGTVLLGAFFIVLANLVVDVAYRFIDPRVGFA"
     gene            8721..9725
                     /locus_tag="VF517_10340"
     CDS             8721..9725
                     /locus_tag="VF517_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8053381.1"
                     /translation="MSALLEVHDLRVGFATEAGTLTAVDGVSFSVEAGGVLGVVGESG
                     SGKSVSSLAVMGLLRGRNVTVTGRATFDGRDLLALGESEMRRVRGNEVAMVFQDPLSS
                     LHPLKRVGAQLVEAVRAHRPLGRAAARARAVELLRMVELPEPERRVDAHPHELSGGMR
                     QRVMIAMALANEPRLLIADEPTTALDVTVQAQILDLLDRLRAELGMALVIVTHDLGVI
                     ARSAAEVAVMYAGRIVERAPTRRLFEEPQHPYTWGLLRSIPRLDSPPGTRLVPIEGRP
                     PSLLARPTGCSFHPRCPHVRESHTRIDPPLEPVDGDPAHEVACLLPARERRELWRSAR
                     "
     gene            9722..10507
                     /locus_tag="VF517_10345"
     CDS             9722..10507
                     /locus_tag="VF517_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8053382.1"
                     /translation="MSLLEVRDLVKEFPSARRAGGRRGEAVRAVDGVSFDVRPGETFG
                     IVGETGSGKSTIARLVARLIEPTAGSIRFDGREVTTLRRGELKALRRDVQMVFQDPYS
                     SLNPRKTVGSIVAEPLAVHGLHEGRGERRRAVQELLERVGLNPEHFNRYPHEFSGGQR
                     QRVGVARALALRPRLILADEPVSALDVSIQAQILNLLRDLSEELGLTLVLISHDLSVI
                     RYMCRRVAVMRAGKVVELADAETLFARPSHPYTAALLEAVPRI"
     gene            complement(10544..11119)
                     /locus_tag="VF517_10350"
     CDS             complement(10544..11119)
                     /locus_tag="VF517_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053383.1"
                     /translation="MLVWLRLKEVKRDRQAQVIDAEEERRVLRSRGSLVRVRTRASSK
                     GGPTAMRRERTRNPDKCGFQIGDTCSRVRDLLLARGRFAGAPPLNVVCLTGRLVDVPD
                     ARIPLSQGWSNRLLKVPRRGLWGGEEPGAFDVNIVLPPELASKATRGAAEHGIVAVVG
                     MLSVDIDYSAGRPHIQYAVVAESIERLGSLD"
     gene            11224..11928
                     /locus_tag="VF517_10355"
     CDS             11224..11928
                     /locus_tag="VF517_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8053384.1"
                     /translation="MDLVAVIKVGPGKEISLTKPVDITDPTVAKAYSHPLRIEILGLL
                     DNRVASPRQLATELDAGLSVTSYHVRQLKGAKLIRLVRRKQVRGAIEHYYTANVRPTI
                     TDTGWGRLPPIVKRAWLGGKIALIGKEVAAAAGAGGFDHKDIHLSRTSVRVTPAAWNA
                     AAKILARALDEIEALGVEGAAHPEAVEEEGEERHAIAVMMLFEAASPESFDPHTGAQS
                     RTENFDDFASTDQHRK"
     gene            12050..12577
                     /locus_tag="VF517_10360"
     CDS             12050..12577
                     /locus_tag="VF517_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8053385.1"
                     /translation="MLCSEPWVDRLISAHSSAEALQVARRYRPHVAVIDTQLVWESGD
                     LCHELRGQSPSTRILLTSGERVSEVNAKSLGAVGVVPKTWHGRAIAAAARSVGFGRTV
                     FAAGPEQQTDLLTPREKTILEMLARGATSRAIATRLNLSPHTVKDHVRSVLRKLKARN
                     RAEAVLRARRLGVLT"
     gene            complement(12669..13529)
                     /locus_tag="VF517_10365"
     CDS             complement(12669..13529)
                     /locus_tag="VF517_10365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8053386.1"
                     /translation="MAVTSRRSSASGCSKRTEQFGFRIVQRGETVPTGDALVTYEAFA
                     PYYDAFTRDYQYESWMSDIDAWARECGLAGRRLLDAACGTGNSFIPMLDRGYEVVACD
                     LSPAMVSRARRKANGRAEVVVADLRALPWCERFDLVTCVDDSLNYLLSTADLVAALRS
                     IHDALEPGGLAAFDFNSLSAYRTTWAGDFTLEAGHRRFRWRGEGSEHMPPGGLASATI
                     ELLAAGETLVTRSRHVQRHHSIETIELACSEVGLEVIEMRGESPEEGLVPEPDEDAHL
                     KIACMARRRK"
     gene            complement(13703..14281)
                     /locus_tag="VF517_10370"
     CDS             complement(13703..14281)
                     /locus_tag="VF517_10370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX8053387.1"
                     /translation="MVREEFLARWDAFNDPELAMLLTWPTSGAPTEIPSRPPYTREKR
                     EAAWEIISRSSSIAAFDVRKADDQRFIGEAGLNHITWPSASADIAVAILDPKDRGSGC
                     GSEVAMLLAAYAFDALGLHRVTLRYLAMNDAVVRGVERDGEAVGARIVGVERQSEWAF
                     GGWQDTVLVEILASDFPPHPATTHLRAAHDST"
     gene            complement(15019..15327)
                     /locus_tag="VF517_10375"
     CDS             complement(15019..15327)
                     /locus_tag="VF517_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053388.1"
                     /translation="MVKVQLIIDLVTPGVGRRRAERTAMLPGMPAVGQFIDTVRGPTL
                     ALAVTAVRWHAVDGTVLAFLGAGDSQRPSITDHDGELELSDHFINDLQDAGWSIGEYE
                     "
     gene            complement(15388..15678)
                     /locus_tag="VF517_10380"
     CDS             complement(15388..15678)
                     /locus_tag="VF517_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053389.1"
                     /translation="MSERGMYVRLADVPVPRRLWWWLLFLFLVVRSNRRALKAAGKTG
                     QDLGESVATRWAQVDADTAAMLRLTQTMVRLTWAVVGLTVVVIAATMYLGLR"
     gene            15763..16143
                     /locus_tag="VF517_10385"
     CDS             15763..16143
                     /locus_tag="VF517_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX8053390.1"
                     /translation="MLPSDPGVTVLSAYAAPDIAAEGGRDNATENGASGRPSIPMLDL
                     FAGAGDLSIGLSREGFEPVGAVEVMDDAALTYEARHDFTVDRRRLEKIPENELRVAWQ
                     GSGRRWGSSLPAVAHRRAAPRRRG"
     gene            16121..16486
                     /locus_tag="VF517_10390"
     CDS             16121..16486
                     /locus_tag="VF517_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX8053391.1"
                     /translation="MRLGDGDDRDGFTAMFRALDLIRPDAFIIENVAGLERGKTRPYF
                     LELVHVLDDVLGYEVKTKTLNGLRARHRGPIRVARPRARPSDPTCRNGRERSRVALDE
                     PEAQVVVGDPAVGACRAEA"
     gene            16811..>17833
                     /locus_tag="VF517_10395"
     CDS             16811..>17833
                     /locus_tag="VF517_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053392.1"
                     /translation="MVGEPRTARGVRYVRLEALTIRGGRGRPFQATPPTPRKRPSRTA
                     APAVPPSNSRSQANLRATLRLLRLEPRARLFFGSYAQSALGNGAGYVALVVLAYQAWH
                     SPWAISLVLLADFLPAMLFGPLFGAAADRWSRRRIAILSDLARAVAFIGIAFVGGIWA
                     IVGFALLAGAGAGLFTPAILAALPSLIEDEERLPAATSLYGALTDAGRSIGPALAGLG
                     LLVASPEAVAAANGVTFLVSAAVLAVLSFGGSPEGASERAGLLREAREGITATARLSG
                     IRVLIGASSGLLLFAAMLNVTELLVAEDLGSGDTGYSVLVAAAGLGFVAGSLAGANGA
                     PIAELKR"
BASE COUNT         2657 a         6414 c         6099 g         2663 t
ORIGIN      
        1 ctgaagggcc agctcaagca ggccacgcgg accggagcgc gcttcgtggc cgtgctgcgc
       61 gaggatgcga ccgtgctcca ggacaccgac acccgcgagg aagtcccgct cgaaggcggc
      121 ccggacggcg tcgtcgccgc cgtgatgcgc cacctgggga ccggatcgct gtgagaagcg
      181 ccccgcgcga gaacgcctac cgcgaggcct ggaccggcga gctgcgctct gccgacgtcg
      241 gccggaccgt cacggtcgcg ggctgggtgc accgccgccg cgaccacggc gggctgatct
      301 tcatcgacct gcgcgaccgc acggggctgc tccagatcgt cttccacccg gagtccgcac
      361 ccgaggcgca cgccgccgcg cacgacctgc gcgccgagga cgtgatctcg gtcggcggcc
      421 gcctgacgcg ccgcgaggag ggtcaggtca acccgaacct gccgaccggc gagatcgagc
      481 tgaccgtcac ggagctcgag cagctcgccg acgccgacac cccgccgttc caggtcgacg
      541 acgagggcac cgtggccgag gagctgcggc tgcgctaccg ctacctcgac ctgcgccgcg
      601 accgcatgcg ccaggcgatc gagctgcgcc accgcgtggt ggccgccatc cgcgaccacc
      661 tcaacgccca ggacttccta gagatcgaga ccccgatgct cacgcgctcg acgcccgagg
      721 gcgcgcgcga ctacctcgtg ccgagccgcg tgcagcacgg ctcctggtac gcgctgccgc
      781 agtcgccgca gctcttcaag cagctcttca tggtgggcgg cctcgagcgc tacttccaga
      841 tcgtccgctg cttccgcgac gaggatccgc gcgccgaccg tcagcccgag ttcacgcagc
      901 tcgacatcga gatgtcgttc gtcgaggagg aggacgtctt cgcgctgatc gacccgctgc
      961 tcgagtcgct gctgggcatc gtcgggatcg aggtctcctt cccgctcgac cgcatgtcct
     1021 acgacgaggc gatcctccgc tacggcaccg accgcccgga ccggcgcccg ggcatggaga
     1081 ttcacgacct cggcgaggtc ttcgccggct cggagttcaa ggtcttcgcg ggcgcgctcg
     1141 agtcgggcgg ggtggtgcgc gggttccggg cgagcggggg cgagtggccg cggcggcgct
     1201 tcgacgagct gaccgagcgg gcgaagtcgc tcggcgccaa gggcctcgtg tgggcggtgg
     1261 tcgagcgcgc cgaggacggc tccggcctcg gctggcgctc gcgggtggcg aagttcctcc
     1321 aggccgacga gatggcgcgc gccgtcgagg cgctcggtgc gggggagggc gacgtgttgc
     1381 tgctcgtggc cgacgacgcg aaggtggccg cgcgcgtgct cggcgacctg cggctcgagg
     1441 tcgccggccc gcaggagggt cacgacctgc tctgggtggt cgacttcccc atgttcgagt
     1501 ggaacgaggg ggaggggagg ctcgacccgc tgcaccatcc gttcacgtcg ccgggcacgg
     1561 acctcgacga cgacccggcg aagtggcgca gccgcgcgta cgacgtgatc atggacggct
     1621 gggagctcgg cggcgggtcg atccgcatca gcgacatcga cgtgcaggag cgcgtcttca
     1681 ccgcgatcgg gctcgctccc gaggaggcgc gcgagcgctt cggcttcctg ctcgaggcgt
     1741 tccgctacgg ggccccgccg cacggcggca tcgcgttcgg tctcgatcgg atcgtggcca
     1801 tgctcgccgg gcgcgagtcg atccgcgacg tgatcgcgtt cccgaagacc tccaccgcgc
     1861 aggacctgct caccggcgcg cccgcgcccg tggacgaggc gcagctcaag gacctcgggg
     1921 tcgcgctgcg caagccgccg ccgggcgcgt gaacgcggct ccggcccggc cgccccgctg
     1981 gtagctttcg cggccctggc ttgctcgcca gccgcccggt tcggttgagc caacaccacc
     2041 gaaacagggt gccccactcg ggccgggtcg tggcccgtcc cggagggcgc ccgaacttgc
     2101 tgaagcaggt tcaacacaag ggcgacagcg gcaagccggg gctcttggat ccggtgcgcg
     2161 gggctcttgc gtgcggcgcg cggggcctcc cggcttccgg cgcgcggggg ctctcggctg
     2221 cggcgcccgc gggctctcgg ctccggcaag ccgggtgctt ctctttaata cagcgcagga
     2281 actcgcaaac ctcgtgtcgc acggcctctc gatcccgccc ttggaggacc atctcgccgc
     2341 tccgcgcgga cggggccggc tacgcgatgg cgctcacgcc ggcagcgccg gcggcgcgcc
     2401 gtgcggcgat ctcatccgga tcgcgctcga ggtccgcgac ggccgcgtgg ccgacgccgg
     2461 cttcgacgcg agcggctgct ccgccgctca ggcggcgggc agcgccgtgg tcgagctggt
     2521 ccgcggccgc ccggtgctcg atgcggcgcg cgtcgcgccg gcgaccgtga gcgacgagct
     2581 cggcggcctc tcgcccgagc gccaccacgc ggcggcgctc gccgcggacg cgctgcaccg
     2641 cgccctcgcg gcggccgcgg cggaccccgc cgcgctgctc gcgcccggcg tcgtcggcga
     2701 cccggccgcg acgcccgcat cgccgctcgc gggccgcacc ctcgtcgcca tgtccggcgg
     2761 cgtggacagc gctgtggccg cgcacctctg ccgcgagcgc ggcgacgacg tcgtggcggt
     2821 caccctcgag ctctgggccg acgccgcgaa cgacggcacg cggagctgct gctcgccgca
     2881 ggccgtcacg catgcccgcg cgctcgcgca ccgcatgggc cttccgcacc tcacgctcga
     2941 cctgagcgag gcgttccgcc acgacgtggt cgacgacttc ctcgccgagc acgccgccgg
     3001 caccacgccc aacccgtgcg tgcgctgcaa cggcctcgtc cgcttcggcg cgatgctcgc
     3061 cctggccgag cgggtcggcg ccgagcgcct cgtcaccggc cactacgccc gcatcgcgcg
     3121 cgacgagcag ggtccgctcg tgcgccaggc cgccgacccg ctgaaggacc aggcctacat
     3181 gctcgcccgc ctcgacccgg agctgctcga ccgcctcgag ttccccctcg gcgacctcgc
     3241 caagcccgag acgcgcgcca tcgcccgcgc cgccggcctg ccggtggccg accgccccga
     3301 cagccaggac ctctgcttcc tcgcgggcac caccgcgcag gcgttcatgg gccgccacgc
     3361 cggacccgcc gcgcgccccg gtgagatcgt cgacgagcgc gggcgcgtgc ttggcacgca
     3421 cgacggccag caccgcttca ccgtcggtca gcgccgcggc atcggggtct cctcgcgcga
     3481 gccgctctac gtggtgcgca aacacgccgg ctcgaaccgc gtggtcgtcg gaccgcgcgc
     3541 cgccctggcg cgccgctcga tcgcgctgag cggcgcgacg ctgcaccgcg acggcgccga
     3601 ggtcgatcgc gtgaagctgc gctaccgctc ggaccccctg ccctgccgcg tcaccgccgc
     3661 cgcgcccgcc gggcgccacg gccgcctcga gctcgagctc ggcgccgagg tgcacggagt
     3721 cgcgcccggc cagacggcct gcctcatgcg acgcgacgcg gtggtcggat gggcgaccat
     3781 tcgccccgac gagatgccca tgccggattc ccattcccta ccgatgagcg aggtggccct
     3841 tgccccgtga caggatcgag ctgacgacgc cggtcggacg cgcctgggag gcggtggtcc
     3901 ggatggtgct cggcggcatc gccgaccggc tcgacctcgg cttcgaccag ctcgacgacc
     3961 tccaactcgc ggttgagcgc ctgctggccg agggcgaccc cagggacccg gtctcgctgt
     4021 cgttcgagct gaccgaaggc ggcgtgcgcg ctcgcatcgg gccgctccgc gacaccgcgc
     4081 ttgccgaggc gctccagggc cccgagccac cccccggcgt gctcaccctt ccgcgcatcc
     4141 tgtcgaccgt ggtcgactcg tacgggatcg aggaggtcgc cgacggccac ctgatcgtgc
     4201 gactggagaa gctggtccgg aggcaatgag cacagtgcag ccggtgaccg acgatcgtga
     4261 gctgctccgc cgctaccacg tggacggcga cccgtccgcg cgcgaggagc tggtgaaccg
     4321 ccacctcccg ctggtgcgct cgctcgcgcg ccgctacgcg ggtcgtggcg aggcgctcga
     4381 ggacatcgag caggtgggcg cgatcggcct gatcaaggcg atcgaccgct acgagctctc
     4441 gcgggaggtc gcgctcacga cctacgcgac gccgaacgtg gtcggcgcga tcaagcgcca
     4501 cgtccgcgac aagggctggg ccattcgcgt gccgcgcgcg ctccaggagc tgaacggcaa
     4561 gatggggccg acgatcgagc gactcacctc gaagctcggc cgctcgccga gcatcgccga
     4621 gatcgcggcc gagttcgaga cctccaccga gcaggtgctg gaggcgctcg aggccggctc
     4681 ggcctacgcg ccgctgtcgc tctcgacggg cccgagcggc gacgaggagc tcgacccgat
     4741 ggagacgatc ggcgagctcg acgaggagtt cgaccgcacc gacgaccgca cctcgctcga
     4801 gcccgcgctc gccgcgctgc ccgagcgcga gcgcgagatc ctccgcatgc gcttcgagga
     4861 cggcctgacc cagacgcaga tcgccgagcg gatcggcatc tcgcagatgc acgtatcgcg
     4921 gctgatccgg cgctcgctcg agcgcatgcg caaccagctc acgtaggttc ccgccgcgca
     4981 gcggcgaacc agtggccgtg accaccgcgg ccgccgagat cgtcccgggg cctccgcccg
     5041 acatcgccgc gcgctcgccg ttcgagctgt tctggcgccg cctgcgtcgc gaccggctcg
     5101 ccttcgcggc gctcggcttc atcgtcttcc tgatcgtcgt cgcgctgctc gcgcccgtga
     5161 tcgtggacgt gaccgggtcg cggccgccga acgagacggg caagcgcttc ctcgacccgg
     5221 agttcggcac ggcgacgggc cccagctctg agaacccgtt cggagtggac acgctcggcc
     5281 gcgacgtcct cagccgcgtc ctgtatggcg cccgcgtgtc gcttcaggtc gctctgatcg
     5341 ccaccgcact gtcggtgctg atcggacttt tggtggggct ggtggccggc tactaccgcg
     5401 gctgggtgga cgcggtcctg tcgcgggtca tcgacgtgct gctggcgttc ccgatcctcc
     5461 tgctggcgct cggcatcgcc gcggcgtgct cgttcggcga gggctgcctg cgcggcgtgg
     5521 tcaagccggg actgggcgtg gtgatcttcg tgatcgcgtt cgtcaactgg acctacatcg
     5581 cgcggatcgt gcgcgggcag gtactccagc tccgcgagcg cgagttcgtc gaggcggcgc
     5641 gctcgctggg cgcctccgac gcgcgcatcc tgttccgcga gatcctcccg aacctcaccg
     5701 cgccgctgct cgtctacacg acgctggcga tcccgcagaa catcctcttc gaggcggcgc
     5761 tctcgttcct gggcgtcgga gtccagccgc ccgacgcgag ctggggcaag atgatcgccg
     5821 acgcgacggc ggtcttcgac accgcctggt ggtacatgct cttcccgggc gccgccctgc
     5881 ttctgacggt cctctcgttc aacatcctcg gcgacggact gcaggacgcg ctcgaccccc
     5941 gcgtaggggg gaactgacgc ccccgtcaga ggaaccggcg ccgcgaaggc gaaccccggc
     6001 ccgacgacaa ggagatgaag cgcatgcgca agcagtggct gatggcagcc atcgtcgggg
     6061 tcctggcact ggtcgccgcg ggctgcggtg gcggcgacga cgaggcgaag cagggcggga
     6121 cgatcacggt gctggccgtg aacgacatca tcagcctcga tccgggcgcc cagtactacc
     6181 agtacgacta catggtggtc tcgcagcccg ggcagcgctc gctctacggc tggaaggccg
     6241 aggagaccaa gccgacgcct gacctcgccg cgagcatgcc gcaggtctca gacggcggtc
     6301 gcaccgtcac catcaagctc cggaagggcg tgaagttcag tccgcccgtg aaccgcgagg
     6361 ccacgtccgc cgacgtgaag tacgcgatgg agcgcctgtt cctcccgtcg gtcggcaacg
     6421 ggtacgcgtc ggtctacttc aacgggatcg tcggcgccga ggagttcacc gagggcaagg
     6481 ccaaggagat cgagggcatc caggctcccg acccgcagac cctcgtcctg aagctcgaga
     6541 acccggtcgg cgtgatcgcc aacgcgaacg ccctgacgct gccgggcacc gcgcccgtgc
     6601 cgaaggacta cgcctccaag tacgacaagg gcgagacctc gacgtacggc gagcacctgg
     6661 tgttcacggg cccgtacatg gtcaagaacg acggcaaggg caacatcacc gggttcacgc
     6721 cgaacaagcg gctcgagctc gtccgcaacc cgaactggga caagacgacc gactaccgcg
     6781 cggcctacgt ggaccgcatc gtctcgctcg ccggcaccga cgcgacggtg gccagccgcc
     6841 gcatcctgtc cggcaagggc ctggtgagcg gcgactttgc cgcaccgccg acgcctgtgc
     6901 tcaagcaggc gctgtcgagc cgcaaggacc agctcgacat cgtggacagc cacggcaacc
     6961 gcttcatcgc gctgaacacg aaggtcaagc cgctcgacaa cgtgaacgtg cgcaaggcga
     7021 tcgccgcggt ggtcgacaag gaggagctgc ggctcacccg aggcggcccg acgctcggca
     7081 cggtcgcttc gcacttcatc ccaccgggga tccccggaca cgacgaggcc ggcggcgagg
     7141 ccggcaccgg cgcggacttc ctgagcaagc ctcagggcga cgtcgcgctg gcccaggagt
     7201 acctgcgcaa ggcggggttc gcgagcggcc gctacgaggg gcccgagctg ctgatggtcg
     7261 gcgacgacga ctcgccgccg aaggagaccg gcgaggcgct ccaggcgcag ctccagaaga
     7321 tcggcatcaa gctcaactac cgccaggtgc cgcacaacac gatgatctcg aagttctgcg
     7381 gcacgccgaa ggcggcggtt gcgatctgcc cgaacctcgg gtgggggccg gacttcttcg
     7441 acgcccagag catggtcgac ccgatcttca acggcgagaa catcgcgccg accaacaact
     7501 cgaactacgc gcagctcgac gacccgaagc tgaacgacgc gatggccaag gcgacgatgc
     7561 tgaccgaccc ggcggcccgg gccaaggcgt acggggagct cgaccggcag atcagcgagc
     7621 tcgcgccggt gatcccgtgg ctgtgggaca agcaggtcaa catccggtcg aaggacgtga
     7681 acggcgtgat caacgccttc aacgcggcct acgacctgaa cttcacgtca gtcgagtagg
     7741 gccacggacg ggcagggacc cgtgatcgcc tacctgctca agcgactcgc ctgggtggtc
     7801 gcgctgctgt tcctggtcag cgcgatcacg ttcgtgatct tttacctgat gccgtcggcg
     7861 gaccccgcgc agctccgcgc cggccgccag ccgacgcccg agctggtcga gcggatccgc
     7921 caccagctcg ggctcgacca gccgtggtac gagcagttct ggcgctacat gaaggatctg
     7981 gtcctgcact tcgacctggg tcgcagctac cagagcaacg tgccggtgcg cgagcagctc
     8041 ttcgaccggc tccccgccac cctgtcgctc gcgctcggcg cggcggtgat ctggatcgtg
     8101 gccggcgtcg cggtcggcac ggtgtcggcg atccggcgcc acacgctgct cgaccgggcg
     8161 gcgatgacgg ccgcgctcgt cgcgatctcc gcgccgccgt actggctcgg cctcgtcgcg
     8221 ctctacctgt tcgcgaacga catcggcagg ttccacgtgt tcgagggagc cgccagctac
     8281 gcgccgctca ccgaggaccc gctccagtgg gcgtcgtcgc tcgttctgcc gtggctcgtg
     8341 ctcgcggcct cgttcgcggc gatctacgcg cggctcgtgc gcgcgacgct cggcgaggcg
     8401 atgtcggagg actacatccg caccgcgcgc gcgaagggcc tgcgcgagcg gcgcgtggtg
     8461 gtccgccacg gcctgcgctc ggccatcacg ccggtcgtca ccgcggcggg gctcgacatc
     8521 ggcgtgctgc tcggcggcgc gatcctcgtc gagaccgtct tcaacatccc tggcatcggc
     8581 ctgctcgcct acgagtcgat cctcgacgcc gacctcccga tgatccaagg cacggtcctg
     8641 ctcggcgcgt tcttcatcgt cctggcgaac ctcgtcgtgg acgtcgcgta ccgcttcatc
     8701 gacccgcgcg tcggcttcgc gtgagcgcgc tgctcgaggt gcacgacctg cgcgtgggct
     8761 tcgccaccga ggcggggacg ctcacggcgg tcgacggggt ctcgttctcg gtggaggccg
     8821 gcggcgtgct cggcgtggtg ggcgaatccg gctcgggcaa gagcgtgtcg agcctcgcgg
     8881 tgatgggcct gctgcgcggg cgcaacgtca ccgtgacggg cagggcgacg ttcgacggcc
     8941 gcgacctgct cgcgctcggc gagtccgaga tgcggcgcgt gcgcggcaac gaggtcgcga
     9001 tggtcttcca ggacccgctc tcgtcgctgc acccgctcaa gcgcgtgggc gcgcagctgg
     9061 tggaggcggt gcgcgcgcac cggccgctcg gacgggcggc ggcgcgggcg cgggcggtcg
     9121 agctcttgcg catggtcgag ctgccggagc cggagcgccg cgtggacgct cacccgcacg
     9181 agctctccgg cgggatgcgc cagcgggtga tgatcgcgat ggcgctcgcc aacgagccgc
     9241 gcctgctgat agccgacgag ccgaccaccg cgctcgacgt gaccgtccag gcgcagatcc
     9301 tcgacctgct cgaccggctc cgtgccgagc tgggcatggc gctcgtgatc gtgacccacg
     9361 acctcggcgt gatcgcacgc agcgccgccg aggtggcggt gatgtacgcc ggccggatcg
     9421 tcgagcgtgc gcccacgcgc aggctgttcg aggagcccca gcacccctac acctggggcc
     9481 tcctgcgctc gatcccgcgc ctcgacagcc cgcccgggac gcggctcgta ccgatcgagg
     9541 gccgcccgcc gagcctgctg gcccgcccga ccggatgcag cttccacccg cgctgcccgc
     9601 acgtgcgcga gtcgcacacc cggatcgacc cgccgctcga gccggtcgac ggcgacccgg
     9661 cgcacgaggt ggcgtgcctg ctgccggcgc gcgagcggcg cgagctgtgg aggagcgcac
     9721 ggtgagcctg ctcgaggtgc gcgacctcgt gaaggagttc ccctccgccc gccgcgccgg
     9781 cggccggcgc ggggaggcgg tgcgcgcggt ggacggcgtc tcgttcgacg tcaggccggg
     9841 ggagacgttc ggcatcgtgg gagagaccgg atcggggaag agcacgatcg cgcgcctcgt
     9901 ggcgcggctg atcgagccga ccgcggggtc gatccgcttc gacggccgcg aggtcacgac
     9961 gcttcgccgc ggcgagctga aggcgctccg ccgcgacgtg cagatggtct tccaggaccc
    10021 gtactcgtcg ctcaacccgc gcaagacggt cggcagcatc gtggccgagc cgcttgctgt
    10081 gcacgggctc cacgagggcc gcggcgagcg gcgccgcgcg gtgcaggagc tgctcgagcg
    10141 agtgggactg aaccccgagc acttcaaccg ctacccccac gagttctcgg gcgggcagcg
    10201 ccagcgcgtc ggcgtggcgc gggcgctggc cctgcggccg cggctgatcc tcgccgacga
    10261 gccggtgtcg gcgctcgacg tgtcgatcca ggcgcagatc ctcaacctgc tgcgcgacct
    10321 gagcgaggag ctggggctga cgctcgtgct gatctcgcac gacctctcgg tcatccgcta
    10381 catgtgccgg cgcgtggcgg tgatgcgcgc ggggaaggta gtcgagctgg ccgacgcgga
    10441 gacgctgttc gcgcggccgt cgcatccgta tacggctgcg ctgctcgagg ccgttccgag
    10501 gatctagcgg cgcgctacga agccgagcat cgccggaatg cggtcagtcg aggctgccca
    10561 gccgttcgat ggactcggcg acgaccgcgt actggatgtg tgggcggccg gcggagtagt
    10621 cgatgtcgac gctgagcatc cccaccaccg cgacgatgcc gtgttcggca gccccgcgtg
    10681 tcgcctttga tgcaagttca ggcggaagca cgatgttgac gtcaaaggcc ccgggttctt
    10741 ctcctcccca tagaccccgg cgcggcacct tcagcagccg gttgctccag ccctggctca
    10801 gcggaatacg ggcgtcgggt acgtcgacca gccttcccgt caggcagacg acgttcaacg
    10861 gcggcgctcc cgcaaaacgg ccccgtgcca gcaacaaatc gcgcacacgt gagcaagtgt
    10921 cgccgatttg gaaaccgcac ttatcaggat tccttgttcg ctcgcggcgc atagccgtgg
    10981 gcccgccttt cgacgaagcg cgcgtgcgga cgcggacgag agagccgcgc gaccgaagca
    11041 cgcgcctctc ctcctccgca tcgatcacct gagcttggcg atcacgcttc acctccttga
    11101 gccgcagcca aacgagcaat tgagcaatat atcctgatca atcaggtcag tcaatcgctc
    11161 cccaaaagcc gattaatttg cagccgaaaa gggcttctgc aagctcgtct caggcgccca
    11221 cccatggatc tggttgcagt gataaaagtg ggtcccggaa aggagatcag cctgacgaaa
    11281 cccgtcgaca taacggatcc cacggtcgcg aaggcgtact cgcatccgct gcggatcgag
    11341 atcctcgggc tcctggacaa ccgggtcgcg agtccccggc aacttgcgac cgaactcgac
    11401 gccggcctct cggtcacgag ctaccacgtt cggcaactca agggcgccaa gctcatccgg
    11461 ctcgttaggc gcaagcaggt gcgcggcgcc atcgagcact actacaccgc gaatgtccgg
    11521 ccgaccatca ccgacacagg ctgggggcgt cttccgccga tagtgaagcg agcttggctc
    11581 ggaggcaaga tcgccctgat cggcaaggaa gtcgccgccg ccgccggcgc gggcggattc
    11641 gaccataagg acattcattt gagccggacg agcgtccgcg taacacccgc tgcctggaac
    11701 gccgccgcga agatcctggc acgtgccttg gacgagatcg aggcactcgg cgtggagggc
    11761 gccgcgcacc ctgaggcggt ggaggaggag ggcgaagagc gccacgcgat cgcggtcatg
    11821 atgctgtttg aggccgcgtc gccagagtcc ttcgatcccc acaccggcgc ccagtcccgc
    11881 accgagaact tcgacgattt cgcctcgacg gatcagcatc ggaagtagct ggcgcatggt
    11941 gggcggtggg aaggcccctg agacaggacc cgcgagtccg gacgcaacct cccaactgcg
    12001 cgtcgtcctg gtcaatcccc acgatctgat ccactggggc ttccgatcct tgctgtgcag
    12061 cgaaccgtgg gtcgaccggc tcatcagcgc ccacagctcg gctgaagcgc tccaggtcgc
    12121 ccgccgctac cggccccacg tcgcggtgat agatacgcag ctggtgtggg aatcgggcga
    12181 cctgtgtcac gaactgcgcg ggcagtcgcc gtccactcgc atcctcctca cctcgggcga
    12241 gcgcgtgtcc gaggtaaacg cgaaatcgct tggggcggtc ggcgttgtgc ccaagacatg
    12301 gcatgggcga gccatcgccg ccgccgcccg atccgttggg tttggcagga ctgtgttcgc
    12361 cgccggaccc gaacagcaga ccgacttgct cacgccccgc gagaagacga ttctcgagat
    12421 gcttgcccgg ggcgcgacca gccgcgcgat cgccacgcgg ctgaacctgt caccacacac
    12481 ggtgaaggat catgtgcgct ctgtgctccg gaagctgaag gcgcgcaatc gagccgaggc
    12541 agtgctgcgt gccaggcggt taggggtact gacctagcgg tgcgcgcctc aagaacgctc
    12601 aggctgacgc acgtcggctg ggcgcctcat ccgcacgatg accgtgaacg tggtccggtc
    12661 ggctcgactc acttgcgcct gcgcgccatg caggcaatct tgaggtgcgc atcttcgtcg
    12721 ggctccggca cgagtccttc ctccggagac tccccccgca tttcaatcac ctcgaggccg
    12781 acctccgagc aagccagttc gatggtctcg atcgagtggt ggcgctgtac gtgtcgacta
    12841 cgggtcacga gcgtttcgcc tgcagcgagg agctcgatcg tggcggacgc cagcccaccc
    12901 ggtggcatgt gctcgctgcc ttcgccgcgc cagcgaaatc gcctgtgccc ggcctcgagc
    12961 gtgaagtcgc cagcccacgt cgtcctgtag gccgagaggg aattgaagtc gaacgccgcg
    13021 agcccccctg gctcgagtgc gtcgtggatg gagcggagcg cggcgacgag atccgccgtc
    13081 gagagcaggt agttcagcga atcgtccacg cacgtgacga ggtcgaaccg ctcacaccaa
    13141 ggcaacgcgc ggaggtccgc cacgacgacc tccgctcgtc cgttcgcctt gcgtctcgcc
    13201 cgcgagacca tcgccgggga gaggtcacac gcaacgactt cgtagccgcg gtcgagcatc
    13261 ggaatgaagc tgttgcccgt cccgcatgcc gcgtcgagca gtcgccggcc ggcgagtccg
    13321 cactcgcgcg cccaggcgtc gatgtcggac atccagctct cgtactggta gtcgcgggtg
    13381 aaggcgtcgt agtagggcgc gaacgcttcg taggtgacga gtgcgtcgcc agtaggcacc
    13441 gtttcgcccc gctgaacaat acggaagcca aattgttcag ttctttttga gcaacccgac
    13501 gcgctgctac ggcgcgacgt aacggccacc tcgccaaacg tccggtggcc tgcggagccg
    13561 cgaccgtcgt gcgttcgcct agcccgcccc tcgacgtcgc tcgcgtcact gcggcggagg
    13621 gatttcctct aggcgcaacc tgggtacgca gacgtggacg aacccccggc cgttctacgt
    13681 tgcgcgttgc gagcgggtcc cactaggttg agtcatgcgc cgcgcgcagg tgggttgtcg
    13741 ccggatgcgg cgggaagtcg gaggccagga tctcgacgag cacggtgtcc tgccatccac
    13801 caaatgccca ctcggattga cgctcgacgc cgacgatccg ggcgcccaca gcctcgccgt
    13861 cccgctcgac gccgcgaacg accgcgtcgt tcattgccag gtagcgcaag gtgacccgat
    13921 gcaaaccgag cgcgtcaaag gcgtaggccg ccaacagcat tgccacctca ctaccgcagc
    13981 cgctcccgcg atccttcgga tcgagtatcg ccacggctat atcggccgag gccgacggcc
    14041 acgtgatgtg gttgagtccg gcctccccga tgaagcgctg gtcatcggcc ttccgcacgt
    14101 cgaatgcggc gatgctcgat gacctgctga tgatctccca ggcagcctcg cgcttctcac
    14161 gcgtatacgg aggcctggac ggtatctccg tcggagcgcc ggacgtgggc catgtgagca
    14221 gcatcgcaag ctcgggatcg ttaaaagcgt cccagcgggc aaggaattcc tcccggacca
    14281 tcgccgaaag gccaactctc tcccctagaa cgatccagcg tcgctgcact taggccggga
    14341 cgatgccaga tcgttagaaa ttgcgtggcc gggtttgcga ccaacggtcg cactgcggtc
    14401 gtcaccgccg gcggtcgctt ccaggcacgt caactgcgac agcccgccag cccgggcgtg
    14461 gttactcgcc taatgcgcct cctagcgcca aggctcccag gagtaccggc ccgtttcgat
    14521 caaggcgatg tggaccgtgt cgccccttgt catcgaacgc gcggaatcaa ggcaagcgca
    14581 cgatgacgtt cggctgccgc ccctcgatga tcgccaagac gacctctagg gcctgcgcgg
    14641 tcgccggcac gaggatgacg aaccaccgcc atatgtagaa gcgcagcttc cactcggctc
    14701 ggcggacgtc tatgtatctg tcttcgtcac ttcgccttag caggcgacgt gacgagggcg
    14761 tgcctgacaa ggcgcgttct cccttctcgt ctacgccacc tgcgggtacc ggagacgcac
    14821 ggagcgcctc ccgatgcgag gaccggagac gcgcggagca tctcccgatt caactcacat
    14881 cgcctcgcct aaccgagctt taggttctgc ccccttgcgg aacaagcgtt agcttccgac
    14941 ccggctggtg agctatgtga ttgcttgccc agccaacttt agggcgcgca cccttggctg
    15001 gcaaccggcg ggacgcgctt actcgtactc cccaatactc cagccagcgt cctgtaggtc
    15061 gttgatgaag tggtcggaga gctcgagctc gccatcgtgg tcagtgatcg aagggcgctg
    15121 cgagtcgccc gccccgagga aggccagcac cgtcccatcg acagcgtgcc accgcacggc
    15181 cgtcaccgcg agggcgagcg tcggacctcg gacggtgtcg atgaactggc cgaccgcagg
    15241 catgccgggc agcatcgccg tgcgctcggc gcgacgccga cccacgcccg gcgtcacgag
    15301 gtcgatgatg agttggacct tgaccatgaa gggtcaatag cttggccagc gggccggatg
    15361 taaggaccca tgcggccctg cgacgcctca gcgcagcccg aggtacatgg tggccgcgat
    15421 gaccacgacc gtcaggccga cgaccgccca cgtcaggcgc accatcgtct gcgtcagcct
    15481 cagcatcgcg gccgtgtccg cgtctacctg cgcccagcgg gtggctaccg actctccgag
    15541 gtcttgaccc gtcttcccgg ccgccttgag cgcccgccga ttcgagcgga cgacgaggaa
    15601 caggaacagg agccaccacc acagtcggcg cgggaccggc acgtctgcca gccgcacgta
    15661 catcccacgc tcgctcatag cgtcaatcgt tccgcgccgg gcggacgccg atgtgcgcga
    15721 tggagcacca tggcgacgca tgaaccgtcc agtgcgagtg ccatgcttcc gtccgacccg
    15781 ggtgtgacgg ttctgtccgc atacgccgcc cccgacattg ccgccgaggg cggccgtgac
    15841 aatgccacgg agaacggcgc aagcggtcgt cccagcatcc ccatgctcga cctgttcgcg
    15901 ggcgcgggcg acctctccat cggcctctcg cgcgagggct tcgagccggt cggcgcggtc
    15961 gaggtcatgg acgacgcggc gctgacctat gaggcccgcc acgacttcac ggtggaccgg
    16021 cggcgcctgg agaagatccc cgagaacgag ctccgcgtgg cgtggcaggg ttcaggtcgt
    16081 cgctgggggt cctccctgcc agccgtggcc caccggcggg ctgcgcctcg gagacgggga
    16141 tgaccgcgac gggttcacag cgatgtttcg cgcgctcgac ctgatccgcc ccgacgcgtt
    16201 catcatcgag aacgtcgctg ggttggagcg cggcaagacg cgcccgtact tcctcgagct
    16261 cgtccacgtc ctagacgacg tgctgggcta cgaggtcaag accaagacgc tcaacggact
    16321 tcgtgcccga caccgtggac ctatacgcgt ggcccggcct cgcgctcgcc cttccgaccc
    16381 gacctgtcga aacgggcggg aacgctcacg agtagcgctt gacgaacccg aagcgcaggt
    16441 ggttgtgggc gacccagccg taggcgcctg ccgggccgag gcctagctcg gtcccgagcc
    16501 ggtgcagcga gcggccgggg gcgtgaccca gcgcgggttg ggggccggga cagccgagcc
    16561 cgctagtcgt cgggccgcat cggcctgccc tgccggtaga caaccggccc gatctcctcg
    16621 ccacggccgc cccgctcgag gccgttccga ggatctagcc gcgcgctatt cggccgcgcg
    16681 ggcccgacct ttagccattc tcggcctttg cacggactat ttcactcaaa tgggggtgtt
    16741 gacagccctg aacctgcacg cttagtgtgg atctcgcata ggtactggcc cgatcccagc
    16801 atgaagttag gtggtcgggg aacctcggac tgcaagggga gttcgatatg tccgcctcga
    16861 ggcgctaacg attcgcgggg ggcggggtcg cccgtttcag gccaccccgc ccaccccgcg
    16921 caaacgcccg tcgcggaccg ccgcacccgc agtacccccc tcgaactccc gctctcaagc
    16981 caacttgcgc gccacgctca gacttctccg gctcgagccc cgagcccgcc tcttcttcgg
    17041 ctcatacgcc cagtccgcgc tcggcaacgg cgccggctac gtggcgctcg tcgtgctcgc
    17101 ctaccaggcc tggcactcgc cgtgggccat ctcgctcgtc ctgctcgccg acttcctgcc
    17161 ggcgatgctg ttcggcccgc tgttcggtgc cgccgccgac cgctggtcgc gccggcggat
    17221 cgcgatcctc agcgacctcg cccgcgccgt cgccttcatc ggcatcgcgt tcgtcggcgg
    17281 tatctgggcg atcgtcggct tcgccctgct ggccggcgcc ggcgccggcc tgttcacgcc
    17341 cgcgatcctc gcggccctcc cgagcctgat cgaggacgag gagcggctgc ccgccgcgac
    17401 ctcgctctac ggcgcgctta ccgacgccgg ccgctccatc ggcccggcgc tcgcgggcct
    17461 gggactgctg gtggccagcc cggaggccgt cgcggccgcc aacggcgtga ccttcctcgt
    17521 gtcagccgcc gtcctggcgg tgctgagctt cggcggcagc ccggagggcg cctccgaacg
    17581 cgccggcctg ctgcgcgagg cacgcgaggg gatcaccgcc accgcgcgcc tgtcggggat
    17641 ccgcgtgctg atcggcgcct cgagcgggct gctgctgttc gcggccatgc tgaacgtgac
    17701 tgaactgctc gtggccgagg acctcggctc gggtgacacg ggctactccg tgctggtggc
    17761 ggcggcgggg ctcggcttcg tggccggctc gctcgccggg gccaacggtg cgccgatcgc
    17821 cgagctcaag cgc
//
LOCUS       DASVPL010000199        18339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_102694, whole genome shotgun sequence.
ACCESSION   DASVPL010000199 DASVPL010000000
VERSION     DASVPL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18339
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102694"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VF517_10400"
     CDS             <1..222
                     /locus_tag="VF517_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053393.1"
                     /translation="DCVREMVRMAVEELASIGDEVFVLARQRNLETIHQHDVVAERHG
                     LLGDLAREIAGDDTPTRHVGLREWLENAA"
     gene            288..737
                     /locus_tag="VF517_10405"
     CDS             288..737
                     /locus_tag="VF517_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053394.1"
                     /translation="MEDRLKTVGLSQDAAIVLALADTAVPFAHSPEDEAERWVRVMRL
                     HGAVGSTLQSLGVGEAPLETSAQPASVRLLRRRPLGEDVVNMVTASARMFAISRGAVT
                     VCTIDVLFALFEVYGKTFDRALYIRGTTREELLERLPQELEASVSQG"
     gene            complement(734..1333)
                     /gene="pgsA"
                     /locus_tag="VF517_10410"
     CDS             complement(734..1333)
                     /gene="pgsA"
                     /locus_tag="VF517_10410"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX8053395.1"
                     /translation="MFPLNLPNALTLFRILLVPVLVVALLDATPSAKWLAAAVFATAA
                     FTDGLDGYLARSRRSETTFGKVMDPIADKLLIAAALIALVSIDRLAGWVAMVIIAREF
                     AVSGLRIAAGAQGVVIPASSLGKVKTASQIAAILALIAASDTGDWWVQALLYTAVAAT
                     LISGADYFLNFRRRIDEARERVAAGAERKAGLDRATPPG"
     gene            complement(1344..1610)
                     /locus_tag="VF517_10415"
     CDS             complement(1344..1610)
                     /locus_tag="VF517_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053396.1"
                     /translation="MTYFKFVRIYSIVELTVFSALLVVWIGGLDEHAKTILGWTHGFG
                     WIALCLLVFEGCRRRVFPWPVLAATVSPLGPLGSTAAIEYVARG"
     gene            complement(1607..2308)
                     /locus_tag="VF517_10420"
     CDS             complement(1607..2308)
                     /locus_tag="VF517_10420"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX8053397.1"
                     /translation="MARIREHARRIGARLGAARFVLVRMALLSAGLLVLWIGLRLAGV
                     EMSVGAIEDWGEELGLAGWLAFVPVAIVLNCVFILAIPIAAAGAGLLFGIVPGTLLAI
                     AVATASTGIQNAIGRRWVGERAEHLLGRRGKQLDDLLDRRGFEAVFYARLTPFTPFTA
                     LNYASGVTRLGAWQIALASGLSMGPRIYAYTALGDNIDDLWSRENVVPFSIIVVSAAI
                     GFALIGRAAIQHRRA"
     gene            2348..2815
                     /locus_tag="VF517_10425"
     CDS             2348..2815
                     /locus_tag="VF517_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053398.1"
                     /translation="MRRVTTAIAVAALALGAAGCDGDDPDPRADFLKQADRICLRSGV
                     RPKALPNDNRQAAKLLGEEARLRAAVHLKLRALEVPAELRADYARFLRLTGEVASALR
                     RMAAVARGDEPARLAELGRRATLVEEERMRLAERIGFRRCGRPITEPVRSESP"
     gene            complement(2796..3245)
                     /locus_tag="VF517_10430"
     CDS             complement(2796..3245)
                     /locus_tag="VF517_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053399.1"
                     /translation="MTRTQAGIAAAATLLLVVPGAAQAASPQKNSGYSGTTSQGEALS
                     LVTSASGKRVIDLATSLTYRCTGEHDGQSGSFVLDTIKVKSGRFSARQDLHGTSDESV
                     VQGGSGTATGTFKRRGRRATGLIRSQITLNTGETCDSGKVTFTVTLT"
     gene            3346..4380
                     /locus_tag="VF517_10435"
     CDS             3346..4380
                     /locus_tag="VF517_10435"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX8053400.1"
                     /translation="MRLSRRNLLAGLPALGALPFAGRALAADEHAGHGAGHAEGAPTE
                     KTAAHAGHGGHVGTVGTVDHRANGFDPHAILRDFDRGTVRREGGRTVREWTLIAQDKE
                     IEVAPGVKYHAWTYNGRVPGPTLRCTEGERLRINFLNGSKHPHTVHFHGIHPATHDGI
                     PGVGAGNIEPGEEFTYEFDAAPFGLHHYHCHTIPLAEHIAKGLYGTFIVDPKRGRPKA
                     DELVMVQNGFDTNFDRSNEVYAVNTVAFAYMNEPIRVKRGELVRIYLVNLLEYDLVNS
                     FHVHANFFQHFPTGTSLEPVEFTDTIVQGQGQRSILEMRFPFPGRYMFHAHVTEFAEL
                     GWMGFFEVED"
     gene            4382..5593
                     /locus_tag="VF517_10440"
     CDS             4382..5593
                     /locus_tag="VF517_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053401.1"
                     /translation="MATVAPRRGGRRWLAALVPLAVLAAAVLALVLLDAPGLERRGVP
                     AEDLVVDRTELRPGEIELHVRNDGPDPVRIEQAVVNDSFVGFEQDSEKVGRLAATTVT
                     AQYPWIEGQAYEVALLTSTGGTVTHEIESAAESPKADAGFFGVMSLIGLYVGIIPLAI
                     GMLWLPWLRQLHERWIRLAMAVTVGLLAFLGLDAMEEGRAIGAEGAQALGGAALVFVG
                     AAVAYLALAGIDSWLRARRARAREAASARGDAGAASAGATLALLIAIGIGLHNLGEGL
                     AIGSAYAIGSLALGVSLVVGFAIHNTTEGLAIVAPAADEAVPLRRLVLLGAIAGLPVI
                     PGALIGAAAFSTSLTALMLGIGAGAIAQVIVQIAPALRDRAGALLNPLAVGGILLGAA
                     VMYATGLLVSV"
     gene            5596..6258
                     /locus_tag="VF517_10445"
     CDS             5596..6258
                     /locus_tag="VF517_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX8053402.1"
                     /translation="MATRELSHAVEDYAKAIYVLEERAGGPVTNNAIAERLSVTPASA
                     SSMVKKLDGLGLVAHVPYRGVRLTARGRRVALEVLRHHRLLERYLVEELGVPWDRVHD
                     EAEVLEHVLSEDLEARIAAKLGDPRHDPHGDPIPTLEGEIDEDETRALGELEPGESGT
                     FTRVSDADPEKLRYLHDRGIAPGMPLAVVGREPFGGPLRVEIGGHEHALGGELAEAMR
                     VA"
     gene            6392..7024
                     /locus_tag="VF517_10450"
     CDS             6392..7024
                     /locus_tag="VF517_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053403.1"
                     /translation="MERFGPLAGLIFLVLAIVSFAIAGDPTDLDKSTEEYARYWADND
                     TEQVISAIIGCYAAVAFLWFSGSVREVVAGVEPGKARLASIAQAGAAVFSAGLLVNSA
                     IQFAAAQTAEDVSPQATEALGALYENFFFPMAGGMAVFLLAAGIGAVRHGAFDRRLGW
                     MAVVIAVVALTPAGFFGFIASMIWVGLASIVLYRKKDPLGSGAAQPPAPA"
     gene            complement(7029..8756)
                     /locus_tag="VF517_10455"
     CDS             complement(7029..8756)
                     /locus_tag="VF517_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8053404.1"
                     /translation="MRRHVISRGGPTIRLARVRCRPGILCAVRRTLAAAAATAALLAA
                     AGPASAADPNCQLDPNPPAESPRIVRGEVDGHPYDVLLPADYGTTSRRYPVLYLLPGR
                     QYSEHSWLVKSDVAEFTEGFTGGRAAIVVTPSMGAEGWALDYRDGSQDWERFPFERLI
                     PHIDARFRTIADGSHRAVAGFSAGGAGALRWAAHRPDAFAAAGGFSAPVPLVAPDEPY
                     TGPGDVPASAGAGSPGPARGPAGDPYADPKEGCNSGGDAIGLRADDGWFFHGNDVASL
                     AVNLRGLGVYFSSGNGATCGPQDARDPVLLAPAEPAIRQLHDDLDRVLTAAAVDHTDD
                     RLPCGVHNMVTAEQGLHAFWDVMVRSFGRRAPASFDYRSIFADASAWGWTFRADAKRA
                     PEFVEVRGASRDGLTLIGSGTETVVTPPLFEAGERVRVEGAGPAAATADPAGRITLAA
                     DLGQPATTAQFAPGGEERRWVARRIRFVPAEPAPATAASFPSGRPCSSGGTLTIRLRH
                     PGHRERRRSVAVSVNGARVLVRRTRAALARKTVILRNLPGGVLRIGVRVRTTHNRTLK
                     AARTYPACR"
     gene            8755..9432
                     /locus_tag="VF517_10460"
     CDS             8755..9432
                     /locus_tag="VF517_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053405.1"
                     /translation="MRLATIAAAVLAATLALPAGAGAQSAGSLLWEQATRPGVSSGFE
                     LAIEVPGELRQKPMAGVTIKFAPGSRIDADGAPLCTAPREQRVNEGHRAACPAGSEVG
                     VGDATALAGSGTVDADLSFWNVRAPDRDLLNVEQLVNGQRVPPFSGTLGSGSIEFVFA
                     SAQRIARITATVRRNTETVGGEPVAYFRTPPVCTYSRRWKIRARLNFEDKTTRMLRAQ
                     VPCRRPR"
     gene            complement(9449..11308)
                     /locus_tag="VF517_10465"
     CDS             complement(9449..11308)
                     /locus_tag="VF517_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053406.1"
                     /translation="MTLVPRTIRTPLLALPLAAIAALAIAAPPASAETRADWVTLADQ
                     MAAPWPNLQKGEGELIDYMDEYTNPGGKPGGTRYGDALMGYGLIQAGIRTGNRAYVDT
                     GIRSISWATDPNRYFAPDARSDDSVFEQWGVAAAYNLMRTHFAGDPGWEKHRGRWEDW
                     MRKQKTVRFGTGRRFSNHDLVEAVMVVELLDSGLTSDQPDAIVGGARAQVERQVLALV
                     NKQAPDVVGDRNPAFISDPPDMPAAYHALSFGLYSHLVSRLGNRATLRARIVVRRSAD
                     GSWRMAAPDADQAMWGRSQELVWAYPAAAYGAAIAAGLPDTSAVDAARYRELVERSLE
                     RLKSDYPITDQGQLISPGLATDRISVANLLEGYTGAPSMGGLALVFLNQALDEPKASI
                     EGSKSRLAADVDTEAVIGDNEASFGVARRGNLWYAVHASRIRQGRLGTDVRYDLGLVG
                     LKRFENGAWRDVVAHRPNTMGQDKRDSIGPVRVSPGPMLFPAAPATVEPAGGGGFVLR
                     GGFWLDNRNRGVQRRANFRYEPSACGVRQTFTGKRGEVYEMSFFFRDRPTKQGRVLSG
                     PRERVTTTVPFSLRIQANNHRSRPYASAAFGPLYRARLRVRVPKSGRVAFETC"
     gene            complement(11341..13005)
                     /locus_tag="VF517_10470"
     CDS             complement(11341..13005)
                     /locus_tag="VF517_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX8053407.1"
                     /translation="MSAAPPSVSSNPSRLSNLRRLFGHRKDPYAGADPERARRIGGTL
                     WIASSVFAVFLSALSPPTQTELGGWGWLLAAALLLGAAVSGYVLIKRSDLISNDQLYA
                     MSFAAVTLDAAMEFLAGGRDTPWHNFYLLIVLFTAAIHPPRRVAAFLPFYLALASASA
                     FHSGGWSAAETGEVGLESLLTIGIALLTLVVMDGVRDQRVVLEQEGDEARHLAHVDPL
                     TDLGNRRALMIELERETPTAQRPLALALYDLDGFKSYNDSFGHVAGDALLVRLAEALG
                     KSVEGRGHAFRMGGDEFCVTTRLPHEEAEQLILTARDALTERAEHFKIEASYGWTQVT
                     DAKTTPSDILRAADRGMYARKTLSRASAGRQTTDVLLSALAERSADLGHHVHDVRELC
                     QAVADELDLWPEETAPLLQAASLHDVGKVAIPDAILDKPGPLTDDEWDFMRRHTIVGE
                     RILSAAPALTEAAKIVRSTHERYDGWGYPDGLAGHDIPLGARVIAVCDAYDAMVSPRP
                     YRSRVTPAEAIAELENCAGSQFDPRVVTAFKAVHTRTGAAFLARIG"
     gene            complement(13075..14292)
                     /locus_tag="VF517_10475"
     CDS             complement(13075..14292)
                     /locus_tag="VF517_10475"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX8053408.1"
                     /translation="MAADRELDVVLYGATGFVGKLTAEYLARAAPDGTRIGLGGRSRA
                     KLERVRESLGAAASEWELVVADSGDATALAELAARTRVVATTVGPYRKYGLKLVDACA
                     SAGTHYADLTGEPLFMLDTIRHFDAPAKASGARIVHNCGFDSIPSDLGVLLLHEAAGE
                     LTDTTLVVTSVRGGVSGGTIAAMGGTIDEVRSDRALRKVLMDPYALSPDRSAEPDLGD
                     EKDLSGVEHDSELGGWLGPFIMAGVNTRVVRRSNALLDWAYGRRFAYREVMGFGEGVK
                     GRAMALGAAAGTGGLTVAMALPPTRFVLDRVLPDPGEGPSDDMVRNGFFEIEVHARTP
                     SGERWVCDVKAKGDPGYGATSVMLGESALCLALAGEKLPERAGVLTPATGMGVALAER
                     LRAAGLTFEVRRG"
     gene            complement(14732..15133)
                     /locus_tag="VF517_10480"
     CDS             complement(14732..15133)
                     /locus_tag="VF517_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053409.1"
                     /translation="MNARETAVVVIASFGTVLVLVRFGAIWRGAGDRAAPPSWPFSHA
                     MWRGVVRTYPLGALMFPSLLVNYVIAKLDSSPSPGLVAVQAAAVAIAVVAFALYVSVV
                     LYNRPKRIVAPPYRDEPGVLEKRGGGGGSTR"
     gene            complement(15191..15775)
                     /locus_tag="VF517_10485"
     CDS             complement(15191..15775)
                     /locus_tag="VF517_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053410.1"
                     /translation="MTRFEHCYSAREAQELDTADVYDVPAEKAPTSRQKLIEIDPQNG
                     PRWLARCSGDYAGGLTGLYSCPNEHDLCVVIEGAAYILHTATPGAADDVEEYPTVHVL
                     PCRADNLLILGSFDTLTAYGPTGFVWQTESFAHDGFAIHEVRGHELVGVAESPAFGAR
                     EFRVDLRNGAHEGGTYWVSNGPRRRSRVWKRIRR"
     gene            complement(16077..16149)
                     /locus_tag="VF517_10490"
     tRNA            complement(16077..16149)
                     /locus_tag="VF517_10490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16114..16116),aa:Thr,seq:cgt)
     gene            16203..16589
                     /locus_tag="VF517_10495"
     CDS             16203..16589
                     /locus_tag="VF517_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053411.1"
                     /translation="MAMHDKQMRKRNDAVTPFLEPGEQIRASFLGQTPMPPWVFFLIA
                     GYIFVFFQKYRTVVLTDRNVYVMQNAFLRTYKFNGIAHKAPLASARIDSSGSWVSIDG
                     GPKLHVPPFGPIKRGLTDFRAAIGQT"
     gene            16626..16985
                     /locus_tag="VF517_10500"
     CDS             16626..16985
                     /locus_tag="VF517_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX8053412.1"
                     /translation="MLPKRVRRLPSHHLGGGLTLIEASGIRARLLGLSLLPGLPRCHA
                     LLIPRCSSVHTFGMRFALDVLFLGPEHELLRVERDVRPNRVLGCRKSTAVIEVPAGQA
                     APFVRAIRAGALARDRG"
     gene            complement(16982..18118)
                     /locus_tag="VF517_10505"
     CDS             complement(16982..18118)
                     /locus_tag="VF517_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053413.1"
                     /translation="MTAVALPGTEDHPHARMVLGAALSGMPSHAYLFHGPTGTGKRTA
                     ARALAAELLAAGEADGDGARARVRAGTHPDLTWVRPTGAHEMRVSDVDEAVVGAATRT
                     PFESSKRVFVLEGADTMSDAVANRMLKTLEEPPAYVHLILLTDALGQVLETVVSRCQL
                     VRFDPLPAERIASVLSGEGVEPGRAGACGRLALGNLTRARFLASAEGEALREDVERMV
                     LDALAGEERDVRAGEPWAPLLERAEGAGREAAAAVAALGKERLADEPKGRDRSAIERE
                     FDDTAKRDGRRARTEVLDLGLELCALAFRDLVCVAERAEGAVLGSDRVEALAPAARGR
                     DARRLRQAVERCEETRLSLALNVTEELALSALTVRLRRLVGSAG"
     gene            complement(18115..>18339)
                     /locus_tag="VF517_10510"
     CDS             complement(18115..>18339)
                     /locus_tag="VF517_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053414.1"
                     /translation="LALDPEAAAARAGERDRFEDEGEALQRRVAGAYEQLAAAAPGRW
                     RRVAADRDVEAVHADVLAAVEAALASGAPA"
BASE COUNT         2578 a         6575 c         6675 g         2511 t
ORIGIN      
        1 gactgcgtgc gggagatggt ccgcatggcg gtggaggagc tcgcgtcgat cggcgacgag
       61 gtcttcgtgc tcgcccgcca gcgcaacctc gagacgatcc accagcacga cgtcgtcgcc
      121 gagcgccacg gtctgctcgg ggacctcgcg cgggagatcg cgggcgacga cacgcccacc
      181 cgccacgtcg ggctgcgcga gtggctcgaa aacgccgcct gacgcacaag cgtccagaag
      241 cgtccacctg gcgtcacgcc cgccgcgccc gcgccgatca cagggtcatg gaagatcgcc
      301 tgaagaccgt aggcctgtcg caggacgcgg ccatcgtgct cgcgctcgcc gacacggcgg
      361 tgccgttcgc ccactccccc gaggacgagg ccgagcgctg ggtccgcgtg atgcggctcc
      421 acggcgcggt cgggtccacg ctccagtcgc tcggcgtcgg cgaggcgccg ctcgagacct
      481 cggcgcagcc cgcgtcggtc cgcctgctgc gccgccgccc gctgggcgag gacgtggtga
      541 acatggtcac cgctagcgcg cggatgttcg cgatctcgcg cggagccgtc acggtctgca
      601 cgatcgacgt gctgttcgct ctgttcgagg tctacggcaa gaccttcgac cgcgcgctct
      661 acatccgcgg cacgacccgc gaggagctgc tcgagcggct tccgcaggag ctcgaggcga
      721 gcgtctcgca gggctagccg ggcggggtcg cccggtcgag ccccgccttg cgctccgccc
      781 ccgccgccac gcgctcgcgc gcctcgtcga tgcggcggcg gaagttcagg aagtagtcgg
      841 cgcccgagat cagcgtcgcg gcgaccgccg tgtagagcag cgcctgcacc caccagtcac
      901 cggtgtccga ggcggcgatc agggcgagga tcgccgcgat ctggctggcg gtcttgacct
      961 tccccagcga gctggcgggg atcaccacgc cctgcgcgcc ggcggcgatc cgcaggccgc
     1021 tcacggcgaa ctcccgcgcg atgatcacca tcgccaccca gccggccagc cggtcgatgc
     1081 tgacgagcgc gatcagcgcg gcggcgatca gcagcttgtc ggcgatcggg tccatcacct
     1141 tgccgaacgt ggtctcgctg cggcgcgagc gcgcgaggta gccgtcgagg ccatcggtga
     1201 acgccgccgt ggcgaagacc gcggccgcga gccatttcgc gctcggcgtg gcgtcgagca
     1261 gcgcgaccac gagcaccggc acgagcagga tccggaagag cgtgagcgcg ttcgggaggt
     1321 tcagggggaa caccccgctg aggctacccg cgggctacgt attcgatagc ggcagtcgag
     1381 ccgagcggcc cgagcggcga gaccgtcgcg gccagcaccg gccacgggaa cacgcggcgg
     1441 cggcagccct cgaagaccag caggcagagc gcaatccagc cgaagccgtg ggtccaaccg
     1501 aggatcgtct tggcgtgctc gtccaggccg ccgatccaga ccacgagcag cgcgctgaac
     1561 acggtcagct ccacgatcga gtagatgcgg acgaacttga agtaggtcat gcgcgccggt
     1621 gctggatggc cgcgcgcccg atcagcgcaa agccgatcgc ggcggacacg acgatgatcg
     1681 agaacggcac gacgttctcg cgcgaccaga ggtcgtcgat gttgtcgccg agcgcggtgt
     1741 aggcgtagat gcgcggtccc atcgagaggc cggacgccag ggcgatctgc cacgcgccga
     1801 gccgtgtgac cccgctcgcg tagttgaggg cggtgaacgg agtgaaggga gtgagccgcg
     1861 cgtagaagac ggcctcgaac ccgcgccggt cgaggaggtc gtcgagctgc ttgccgcgcc
     1921 tgccgagcag gtgctcggcg cgctcgccca cccagcggcg cccgatcgcg ttctggatcc
     1981 cggtcgaggc ggtggccacg gcgatcgcca ggagcgtgcc cgggacgatc ccgaagagca
     2041 gccccgcgcc cgccgccgcg atcgggatcg cgaggatgaa gacacagttg aggacgatcg
     2101 cgacgggcac gaacgcgagc cacccggcca ggccgagctc ctccccccag tcctcgatcg
     2161 caccgaccga catctccacg cccgcgagcc gcaggccgat ccagaggacc agcagcccgg
     2221 cggacagcag cgccatccgc acgagcacga agcgcgcggc gccgaggcgc gcgccgatcc
     2281 tgcgcgcgtg ctcgcggatt cgggccacga acggagccta ttcggcgccg atagcctcgc
     2341 cgccgccatg cgacgggtca ccacagcgat cgcggtcgcc gcgctcgcgc tcggcgcggc
     2401 gggctgcgac ggcgacgacc ccgacccgcg ggcggacttc ctcaagcagg ccgaccgcat
     2461 ctgcctgcgc tcgggcgtgc gccccaaggc gctgccgaac gacaaccgcc aggcggccaa
     2521 gctgctcggc gaggaggcgc ggctgcgcgc ggcggtgcac ctgaagctgc gcgcgctcga
     2581 ggtgccggcc gagctgcgcg cggactacgc gcggttcctc aggctgaccg gcgaggtggc
     2641 gagcgcgctg cggcggatgg ccgcggtggc gcgcggcgac gagccggcgc ggctcgccga
     2701 gctcgggcgg cgcgcgacgc tcgtcgagga ggagcgcatg cgcctggccg agcggatcgg
     2761 gttcaggcgc tgcgggcgcc cgatcacgga gccggtcagg tcagagtcac cgtgaaggtg
     2821 accttgccgg agtcgcacgt ctcgccggta ttcagggtga tctgagagcg gatcagcccc
     2881 gtggcgcggc ggccgcggcg cttgaacgtc ccggtggctg tgccggaccc gccctgcacc
     2941 accgactcgt cgctcgtccc gtgcaggtcc tggcgcgcgc tgaagcgacc gctcttcacc
     3001 ttgatcgtgt cgagcacgaa gctgccggac tggccgtcgt gctcgccggt gcagcggtag
     3061 gtaaggctcg tggcgaggtc gatcacccgc ttcccgctcg ccgacgtgac gagcgagagc
     3121 gcctcgccct gcgaggtcgt cccgctgtag ccggagttct tctgggggct ggcggcctgc
     3181 gccgcgcccg gtacgacgag cagcaacgtc gccgccgcgg caatccctgc ctgagtgcga
     3241 gtcatcgatt cctttcgatt cgtcctgcgg cgagcgtacg ccagggtgcg ccgccccgcc
     3301 accttttaga catgactgaa cttgtcgata agcttcgcta caagcatgcg actgtcacgc
     3361 cgaaacctgc tcgccgggct tcccgccctc ggcgcgctgc cgttcgcggg gcgggcgctg
     3421 gccgctgacg agcacgcggg ccacggcgcg ggccacgccg agggcgcgcc caccgagaag
     3481 accgcggcgc acgccggcca cgggggacac gtggggacgg tcgggaccgt cgaccaccgc
     3541 gccaacggct tcgacccgca cgcgatcctg cgcgacttcg accgcggcac ggtgcgccgc
     3601 gagggcgggc gcacggtccg cgagtggacg ctgatcgcgc aggacaagga gatcgaggtc
     3661 gcgcccggcg tgaagtacca cgcctggacc tacaacgggc gcgtgcccgg ccccacgctg
     3721 cgctgcaccg agggcgagcg gctgcggatc aacttcctga acggctcgaa gcatccgcac
     3781 acggtccact tccacggcat ccacccggca acgcacgacg gcattccggg cgtgggtgcc
     3841 gggaacatcg agccgggcga ggagttcacc tacgagttcg acgccgcgcc gttcgggctc
     3901 catcactacc actgccacac gatcccgctg gccgagcaca tcgcgaaggg gctctacggc
     3961 acgttcatcg tcgacccgaa gcggggccgg ccgaaggccg acgagctcgt gatggtccag
     4021 aacgggttcg acaccaactt cgaccgctcg aacgaggtgt acgccgtcaa cacggtcgcg
     4081 ttcgcgtaca tgaacgagcc gatccgggtc aagcgcggcg agctcgtccg gatctacctc
     4141 gtgaacctcc tcgagtacga cctcgtcaac tcgttccacg tccacgcgaa cttcttccag
     4201 cacttcccca ccggcacgtc gctcgagccg gtcgagttca ccgacacgat cgtgcagggg
     4261 cagggccagc gctcgatcct cgagatgcgg tttcccttcc ccggcaggta catgttccac
     4321 gcgcacgtga ccgagttcgc ggagctcggc tggatgggct tcttcgaggt cgaggactag
     4381 cgtggcgacg gtcgccccgc gacgcggtgg acggcgctgg ctcgcggcgc tcgtgccgct
     4441 cgcggtgctg gcggccgcgg tcctggcgct ggtgctgctc gacgcgccgg gcctcgagcg
     4501 gcgcggcgtg ccggccgagg acctcgtcgt cgaccgcacc gagctgcggc ccggcgagat
     4561 cgagctgcac gtgcgcaacg acgggccgga tccggtgagg atcgagcagg cggtcgtcaa
     4621 cgactcgttc gtgggcttcg agcaggactc cgagaaggtc gggcgcctgg cggccacgac
     4681 cgtgaccgcg cagtacccgt ggatcgaggg ccaggcctac gaggtcgcgc tgctgacctc
     4741 gacgggcggc acggtcacgc acgagatcga gtcggcggcc gagtcgccga aggccgacgc
     4801 cggcttcttc ggcgtgatga gcctgatcgg gctctacgtg gggatcatcc cgctggcgat
     4861 cgggatgctg tggctgccct ggctgcggca gctccacgag cgctggatac ggctcgcgat
     4921 ggccgtcacg gtcggcctgc tcgcgttcct cgggctcgac gcgatggagg aggggcgcgc
     4981 gatcggcgcc gagggcgcgc aggcgctggg cggcgcggcg ctggtgttcg tgggcgcggc
     5041 ggtggcgtac ctggcgctcg cgggaatcga ctcgtggctg cgagcgcgcc gggcgcgggc
     5101 gcgggaggcc gcgtcggcgc gcggcgacgc cggggcggcg agcgccggtg ccaccctcgc
     5161 gctgctgatc gcgatcggca tcgggctgca caacctcggc gaggggctcg cgatcgggtc
     5221 ggcgtacgcg atcgggtcgc tcgcgctggg ggtctccctc gtggtgggct tcgccatcca
     5281 caacaccacc gaggggctgg cgatagtcgc gcccgcggcc gacgaggcgg tgccgctgcg
     5341 gcggctcgtc ctgctcggcg cgatcgccgg cctgccggtg ataccgggcg ccctcatcgg
     5401 cgcagcggca ttcagcacga gcctcacggc gctgatgctc gggatcggcg cgggcgcgat
     5461 cgcgcaggtc atcgtgcaga tcgcgccggc gctgcgcgac cgcgccggag cgctgctcaa
     5521 cccgctggcc gtcgggggca tcctgctcgg cgcggcggtg atgtacgcca ccggcctgct
     5581 ggtgtcggtg tagcgatggc aacgcgggag ctctcgcacg cggtcgagga ctacgcgaag
     5641 gcgatctacg tgctcgagga gcgcgccggc gggccggtca ccaacaacgc gatcgccgag
     5701 cggctgagcg tcacgccggc gtcggcgtcg agcatggtca agaagctcga cgggctcggc
     5761 ctggtcgccc acgtccccta ccgcggcgtg cggctgaccg cgcgcgggcg gcgggtcgcg
     5821 ctcgaggtgc tgcgccacca ccggctgctc gagcgctacc tcgtcgagga gctgggcgtg
     5881 ccctgggacc gcgtgcacga cgaggccgag gttctcgagc acgtgctctc ggaggatctc
     5941 gaggcacgca tcgcggcgaa gctaggcgac ccgcgccacg acccgcacgg cgacccgatc
     6001 ccgaccctcg agggcgagat cgacgaggac gagacgcgcg cgctcggcga gctggagccc
     6061 ggcgagagcg gcacgttcac gcgcgtctcg gacgccgacc cggagaagct ccgctacctg
     6121 cacgaccgcg gcatcgcgcc gggaatgccg ctggcggtgg tcggccgcga gccgttcggc
     6181 gggccgctgc gcgtggagat cggcggccac gagcacgcgc tgggcggcga gctggccgag
     6241 gcgatgcgcg tggcgtagcg ccgcccgccg cggctccgcg cgcgtcggtc cttgtacggg
     6301 tcgccccatc cccggcacgg taggccgtcc gggacgcgtg cgagcttgcc cgcaaccaac
     6361 gaacggggga cttgtggccg agcgaaggga catggagcgg ttcggaccgc tggcggggct
     6421 gatcttcctg gtgctggcga tcgtgtcgtt cgcgatcgcg ggcgacccga ccgacctcga
     6481 caagtccacc gaggagtacg cgcgctactg ggcggacaac gacacggagc aggtcatctc
     6541 ggccatcatc gggtgctacg cggccgtcgc gttcctgtgg ttctcgggca gcgtgcgcga
     6601 ggtggtcgcc ggcgtcgagc ccggcaaggc gcggctcgcg tcgatcgcgc aggccggcgc
     6661 cgcggtcttc agcgcggggc tgctcgtcaa ctccgcgatt cagttcgctg ccgcgcagac
     6721 ggcggaggac gtctcgccgc aggccaccga ggcgctgggc gcgctgtacg agaacttctt
     6781 cttcccgatg gccgggggga tggcggtatt cctgctcgcc gccggcatcg gggcggtgcg
     6841 gcacggggcg ttcgacaggc ggctcgggtg gatggctgtc gtgatcgccg tggttgcgct
     6901 cacgcctgcc gggttcttcg gcttcatcgc cagcatgatc tgggtggggc tcgcctcgat
     6961 cgtcctatac cgcaagaagg acccgctggg ctcgggggcg gctcagccgc cggcgcccgc
     7021 ctgagccgct acctgcaggc cgggtaggtc cgggccgcct tcagcgtccg gttgtgcgtg
     7081 gtgcgcacgc gcacgccgat gcgcagcacg ccgccgggaa ggttccgcag gatgaccgtc
     7141 ttgcgcgcga gcgcggcgcg cgtgcgccgc acgagcacgc gcgcgccgtt cacgctcacc
     7201 gcgaccgagc ggcggcgctc gcggtggccg gggtgcctga ggcggatcgt cagggtgccg
     7261 ccgctcgagc aggggcgccc ggaggggaag ctggcggcgg tcgcgggagc gggctccgcg
     7321 ggcacgaacc ggatccgccg ggcgacccag cggcgctcct cgccgcccgg cgcgaactgc
     7381 gccgtggtgg cgggctggcc gaggtcggcg gccagcgtga tccggcccgc ggggtcggcc
     7441 gtcgcggctg cgggcccggc gccctcgaca cgcacccgct cgcccgcctc gaacagcggc
     7501 ggggtgacga ccgtctcggt ccccgacccg atcagcgtca gtccgtcgcg cgaagcgccg
     7561 cgcacctcca cgaactcggg cgcgcgcttc gcgtccgcgc ggaacgtcca gccccaggcg
     7621 ctcgcgtcgg cgaagatcga gcggtagtcg aagctcgcgg gcgcgcggcg gccgaacgac
     7681 ctcaccatca cgtcccagaa cgcgtgcagg ccctgctcgg cggtgaccat gttgtgcact
     7741 ccgcagggca gccggtcgtc ggtgtgatcg acggcggcgg ccgtgagcac gcggtcgagg
     7801 tcgtcgtgga gctgacggat ggccggctcg gccggcgcca gcagcaccgg atcgcgcgcg
     7861 tcctggggcc cacacgtcgc gccgttgccg gaggagaagt agacgccgag cccgcgcagg
     7921 ttcaccgcga ggctggcgac gtcgttcccg tggaagaacc agccgtcgtc cgcgcgcagg
     7981 ccgatcgcgt cgccgcccga gttgcagccc tccttcgggt cggcgtaggg atcgccggcg
     8041 ggcccgcgcg cgggcccggg cgagccggcg cccgctgagg cgggcacgtc gcccggtccc
     8101 gtgtacggct cgtccggcgc caccagcggg accggcgcgg agaacccgcc ggcggcggcg
     8161 aaggcatccg gccggtgcgc cgcccagcgc agcgcaccgg caccgccggc cgagaagccg
     8221 gcgaccgcgc gatgggagcc gtcggcgatg gtgcggaagc gcgcgtcgat atgcgggatg
     8281 agccgctcga acgggaagcg ctcccagtcc tgcgagccgt cgcggtagtc gagggcccag
     8341 ccctcggcac ccatcgaggg cgtcaccacg atcgcggcac gcccacccgt gaagccctct
     8401 gtgaactcgg cgacatcgct cttgacgagc cacgagtgtt cgctgtactg gcggccgggg
     8461 agcaggtaca gcaccgggta gcggcggctc gtggtcccgt agtcggcggg cagcaggacg
     8521 tcgtaggggt gcccgtcgac ctcgccgcgc acgatgcggg gcgactcggc gggcgggttg
     8581 gggtcgagct ggcagttggg gtccgcggcg ctcgccggcc ccgcggctgc cagcagggcg
     8641 gcggtcgcgg cggcggcggc gagcgtcctc ctcaccgcgc agaggatgcc ggggcggcaa
     8701 cgcacgcgcg ccagccggat cgtcgggccg ccgcggctga ttacatgccg ccgcatgcgc
     8761 ctcgccacca tcgccgccgc cgtgctcgcc gccaccctcg cgctcccggc cggcgccggt
     8821 gcccagagcg cagggtccct gctctgggag caggccacgc ggccgggcgt ctcgagcggc
     8881 ttcgagctcg ccatcgaggt cccgggcgag ctgcgccaga agccgatggc cggggtgacg
     8941 atcaagttcg cgccgggctc gcgcatcgac gccgacgggg cgcccctgtg caccgcgccg
     9001 cgcgagcagc gcgtgaacga gggacaccgg gcggcgtgcc cagccggcag cgaggtcggg
     9061 gttggtgacg cgaccgcgct cgcgggcagc ggcacggtcg acgccgacct ctcgttctgg
     9121 aacgtgcgcg cccccgatcg cgacctcctc aacgtcgagc agctcgtgaa cggccagcgc
     9181 gtcccgccgt tcagcgggac gctcgggtcg gggtcgatcg agttcgtctt cgcctccgcc
     9241 cagcgcatcg ctcgcatcac cgcgacggtc cgccgcaaca cggagacggt cggcggcgag
     9301 ccggtcgcct acttccggac cccgccggtc tgcacctaca gcaggcgctg gaagatccgc
     9361 gcccggctga acttcgagga caagaccacg cggatgctgc gcgcgcaggt gccgtgccgg
     9421 cgcccgcgct gagcgccgcg gcgctgactc agcaggtctc gaacgcgacc cggccggact
     9481 tcggcacgcg gacgcgcagg cgtgcgcggt acagcgggcc gaacgcggcc gacgcgtacg
     9541 ggcgcgagcg gtggttgttc gcctggatcc gcaggctgaa cgggacggtg gtcgtgacgc
     9601 gctcacgcgg gcccgacaga acgcggccct gcttggtcgg gcggtcgcgg aagaagaacg
     9661 acatctcgta gacctcgccg cgcttgccgg tgaaggtctg gcgcacaccg caggcgctcg
     9721 gctcgtagcg gaagttcgcg cgccgctgga cgccgcgatt gcggttgtcg agccagaagc
     9781 cgccgcgcag cacgaagccg ccgccgccgg ccggttcgac ggtggccggc gcggccggga
     9841 agagcatcgg gcccggcgag acgcggaccg ggccgatgct gtcgcgcttg tcctggccca
     9901 tcgtgttcgg gcgatgtgcg acgacgtcgc gccaggctcc gttctcgaag cgcttgagac
     9961 cgacgaggcc gaggtcgtag cggacgtccg tgccgagccg gccctgccgg atccgcgacg
    10021 cgtgcaccgc gtaccagagg ttcccgcggc gggcgactcc gaaggaggcc tcgttgtcgc
    10081 cgatcaccgc ctcggtgtcg acatcggccg cgaggcgcga cttcgagccc tcgatcgagg
    10141 ccttgggctc gtcgagggcc tgattgagga acacgagggc gagcccgccc atcgaggggg
    10201 cgccggtgta gccctcgagc aggttcgcca cgctgatccg gtcggtggcg agcccgggcg
    10261 agatgagctg gccctggtcg gtgatcgggt agtcgctctt gaggcgctcg agcgagcgct
    10321 cgaccagctc gcggtagcga gcggcgtcga cggcgctggt gtcgggcagc ccggcggcga
    10381 tcgccgcccc gtaggcggcg gccgggtagg cccagacgag ctcctgcgag cggccccaca
    10441 tggcctggtc ggcgtccggg gcggccatgc gccacgagcc gtcggcggag cggcgcacga
    10501 cgatgcgcgc gcgcagagtg gcccggttgc cgagtcgcga gaccaggtgc gagtagaggc
    10561 cgaagctcag cgcgtggtag gcggcgggca tgtcgggcgg gtcggagatg aaggcggggt
    10621 tgcggtcgcc gacgacgtcg ggcgcctgct tgttgacgag cgcgagcacc tggcgctcga
    10681 cctgcgcgcg ggcgccgccg acgatcgcgt ccggctggtc ggaggtcaga cccgagtcga
    10741 gcagttcgac gaccatcacc gcctcgacaa ggtcgtggtt gctgaagcga cggccggtgc
    10801 cgaagcggac ggtcttctgc ttacgcatcc aatcctccca ccggccgcgg tgcttctccc
    10861 agccggggtc gccggcgaag tgagtgcgca tgaggttgta ggcggcggcc acgccccact
    10921 gctcgaagac gctgtcgtcg ctgcgggcat ccggggcgaa gtagcggttg gggtcggtgg
    10981 cccacgagat cgagcggatg ccggtgtcga cgtaggcgcg gttgccggtg cgaatcccgg
    11041 cctggatcaa gccgtagccc atcagcgcgt cgccgtaacg ggtgccgccg ggcttgccgc
    11101 cggggttcgt gtactcgtcc atgtagtcga tcagctcgcc ctcgcccttc tggaggttgg
    11161 gccagggggc cgccatctgg tcggccaggg tcacccagtc ggcgcgcgtc tcggcgctcg
    11221 cggggggcgc cgcgatcgcg agggcggcga tcgccgccag gggcagcgcg agcaggggcg
    11281 tgcggatcgt gcgcggcacg agggtcacaa cctcattgtg gggcgcgggt tgcggagtgg
    11341 ctagccgatg cgcgccagga aggcggcgcc ggtccgcgta tgaaccgcct tgaaggcggt
    11401 cacgacgcgc gggtcgaact gggatcccgc gcagttctcg agctcggcga tggcctcggc
    11461 gggggtcacg cggctgcggt agggacgcgg cgagaccatg gcgtcgtagg cgtcgcagac
    11521 ggcgatgacg cgggcgccga gcgggatgtc gtggccggcc aggccgtccg ggtagcccca
    11581 gccgtcgtag cgctcgtggg tggagcgtac gatcttcgcg gcctcggtga gggcgggggc
    11641 cgcgctgagg atccgctcgc cgacgatcgt gtggcggcgc atgaagtccc actcgtcgtc
    11701 ggtgagcggg ccgggcttgt cgaggatcgc gtcggggatg gcgaccttcc cgacatcgtg
    11761 cagcgatgcc gcctggagga gcggcgccgt ctcctcgggc cagaggtcga gctcgtcggc
    11821 gacggcctgg cagagctcgc gcacgtcgtg gacgtggtgg ccgaggtcgg cgctgcgctc
    11881 ggcgagcgcg ctcaggagga cgtcggtggt ctgcctgccg gcgctcgcgc gcgagagggt
    11941 cttgcgcgcg tacatgccgc gatcggcggc gcgcaggatg tcgctcgggg tggtcttcgc
    12001 gtcggtcacc tgggtccagc cgtaggaggc ctcgatcttg aagtgctcgg ccctctccgt
    12061 gagcgcgtcc ctggcggtga ggatgagctg ctcggcctcc tcgtgcggga ggcgtgtggt
    12121 cacgcagaac tcgtcgccgc ccatgcggaa tgcatggccc cggccctcga ccgacttgcc
    12181 gagcgcctcg gcgaggcgga cgagcagcgc gtcgcccgcc acgtggccga aggagtcgtt
    12241 gtaggacttg aagccgtcga ggtcgtagag ggcgagggcg agcggccgct gcgcggtggg
    12301 ggtctcgcgc tcgagctcga tcatcagggc gcgacggttc ccgaggtccg tgagcgggtc
    12361 gacgtgtgcg aggtgccggg cctcgtcgcc ctcctgctcg agcaccacgc gctggtcgcg
    12421 gacgccgtcc atgaccacca aggtgagcag cgcgatcccg atcgtgagga ggctctccag
    12481 cccgacctcg cccgtctccg ccgcgctcca gccgccggag tggaaggcgc tggcgctcgc
    12541 gagagcgagg tagaacggga ggaaggcggc gacgcggcgc ggcgggtgga tcgccgccgt
    12601 gaagaggacg atgagcaggt agaagttgtg ccacggcgtg tcgcgccccc cggcgaggaa
    12661 ctccatggcg gcatcgagcg tgacggcggc gaagctcatc gcgtagagct ggtcgttgct
    12721 gatgaggtcg ctgcgcttga tcagcacgta gccgctgacg gcggcgccga gcaggagcgc
    12781 ggcggcgagc agccagcccc agccgccgag ctcggtctgc gtggggggcg agagcgccga
    12841 gaggaagacc gcaaacaccg aggacgcgat ccacagggtc ccgccgatgc gccgcgcacg
    12901 ctccgggtcg gcaccggcgt acgggtcctt gcgatggccg aagagacgcc ggaggttgct
    12961 caggcgcgag gggttgctgg aaacggacgg cggggcggcc gacatgctgg gggtgttgtc
    13021 ggcaggttgg gcgcgtgtgt gtaccccctg cgggggacac gcgccattcc agggctagcc
    13081 ccggcggacc tcgaacgtca gtccggcggc gcgcagccgt tccgccaggg ccacgcccat
    13141 cccggtggcc ggcgtcagca cgcccgcccg ctcgggcagc ttctccccgg ccagagccag
    13201 gcagagtgcg ctctcgccga gcatcaccga ggtggccccg tagcccggat cgcccttggc
    13261 cttgacgtcg cagacccagc gctctccgct cggcgtccgg gcgtgcacct cgatctcgaa
    13321 gaagccgttg cgcaccatgt catcgctcgg accctcgccc ggatcgggca gcacgcggtc
    13381 gagcacgaag cgcgtgggcg gcagcgccat ggcgacggtg aggccgcccg tgccggcggc
    13441 ggcgccgagc gccatcgccc ggcccttgac gccctcgccg aaccccatca cctcgcggta
    13501 cgcgaagcgc cgaccgtaag cccagtcgag cagcgcgttc gagcggcgca ccaccctcgt
    13561 gttgaccccg gccatgatga acgggccgag ccaaccgccc agctcgctgt cgtgctcgac
    13621 tccgctcagg tccttctcgt cgcccaggtc cggctcggcc gagcgatcgg gcgacagggc
    13681 gtaggggtcc atgagcacct tccgcaacgc acggtcggag cgcacctcgt cgatcgttcc
    13741 gcccatcgca gcgatcgtcc cgccgctgac gccgccgcgc acgctcgtga cgacaagggt
    13801 cgtgtcggtg agctcccccg ccgcctcatg caggagcagc acgccgaggt ccgaggggat
    13861 cgagtcgaag ccgcagttgt gaacgatgcg cgcgccgctc gccttcgcgg gcgcgtcgaa
    13921 atgacggatc gtgtcgagca tgaacagggg ctcgccggtg aggtccgcgt agtgggtgcc
    13981 ggcgcttgcg cacgcgtcga ccagcttcag cccgtacttg cgatagggcc cgacggtcgt
    14041 ggccaccacc cgcgtgcgcg cggccagctc agccagcgcc gtggcatccc ccgagtcggc
    14101 cacgaccagc tcccactccg acgcggccgc gccgagggac tcgcgcacgc gctcgagctt
    14161 cgcgcgcgag cgcccgccca gcccgatccg cgtgccgtcg ggcgcggcgc gcgcaaggta
    14221 ctccgccgtg agcttgccga cgaagccggt ggcgccgtag aggacgacgt cgagctcgcg
    14281 gtcggctgcc acagccacgg cacgctaccg cagcgagcgc tcgcgccaga gtcggccagg
    14341 agacccgacc ccctacccgg gccgatcaat tcccccatcg ggctgagaag tcgctgccct
    14401 ccgcccggcc ccaccccacg acggcacgcg gtacgccgcc cagcttacgt agagccgacg
    14461 aggggactcg aacccctgac cccttcatta cgagttggcg cctggagggc gggttgtgct
    14521 gggttgagcg gggctcgatc ccgttgcgcc gcgttctcgg aggtcagaat tgccgagttg
    14581 gggacatggt tcgggacacg ctttcgcagt cggcggcgcg ccgcgcgatc ctcgcgccca
    14641 agtgccctcc caaagcgact tggacgcgtc aggcggagtg cttagccaac caacgcccgg
    14701 tcgccgcgcg gtggacctac cgcgccaccg tctaccgggt gcttcccccg cccccgccgc
    14761 gcttctccag cacgcctggc tcgtcgcgat agggaggtgc cacgatgcgc ttcgggcggt
    14821 tgtagaggac gaccgagacg tagagcgcaa acgcgaccac ggcaatcgcc accgcggccg
    14881 cctggaccgc cacgagccct gggctcggcg acgagtccag cttcgcgatc acatagttca
    14941 cgagcaaaga cggaaacatc agggcgccga gcgggtacgt cctcaccacg ccacgccaca
    15001 tcgcgtggct gaacggccag ctcggcggcg ccgcccggtc gccggcacca cgccaaatgg
    15061 ccccgaaacg cacgaggacc agcaccgtcc cgaaggacgc gatgactacc acggccgttt
    15121 cacgcgcgtt catacgcagt gtccgagtcg gtccagtttg gccgacgcac gttcctcttg
    15181 tcggccaggt tcagcgtcga atccgcttcc agacccggga ccggcgtctc ggtccgttcg
    15241 aaacccagta cgtacctccc tcgtgggcac cgtttctgag gtcgacccga aactcccgcg
    15301 caccgaacgc cggagactcc gcgacgccta ccaactcgtg cccacgcacc tcgtgtatcg
    15361 cgaaaccatc gtgcgcaaac gactctgtct gccagacaaa tccggtcggt ccatacgcgg
    15421 tcagcgtgtc gaaggagcca agaatgagca gattgtcggc acggcacggc aacacgtgga
    15481 ctgttggata ctcctcgacg tcatcggccg ctcccggcgt ggcggtgtgc agaatgtagg
    15541 cggcgccctc gatgacgacg cagagatcgt gctcgttcgg acacgagtag aggcctgtca
    15601 gtccgccggc gtagtcgcct gagcagcgcg caagccaccg ggggccgttc tgcgggtcta
    15661 tctcgatgag cttctgacgg gaagtcggtg ccttctccgc gggcacgtcg tagacgtcgg
    15721 ccgtatcgag ttcttgagcc tcgcgtgccg agtagcaatg ctcgaaacgt gtcatcgaac
    15781 gggatcattg accggggagc ttgttctttc tgaagaaccg atgggtgatc tgaccaccct
    15841 ctatgccgat ctcgtaccga cccgacttcc cgcggtgaac gccgccattt ggcactcacg
    15901 gtacctcccg ccgcgagtcc atcgctgttc ttcctatgcg tgatagcgcg aaagcgcaag
    15961 agtggatttg agaagaaccc tcaaatcgga gagcccacgg tgcaccacat gccggggttc
    16021 cgtcaggcca gcctcaaatc gcttcacgca cttgcactca gtgctctcag tactagagcc
    16081 gacgagggga ctcgaacccc tgaccccttc attacgagtg aagtgctcta ccagctgagc
    16141 tacgtcggcg ggtcgggccg cagtctagac acggcaccgg gccgcgccat aggctctcgc
    16201 acgtggccat gcacgacaag cagatgcgca agcgcaacga cgccgtcacg ccgtttctcg
    16261 agcccggcga gcagatccgc gcgtcgttcc tcggtcagac ccccatgccg ccgtgggtgt
    16321 tcttcctgat cgcgggctac atcttcgtct tcttccagaa gtaccgcacg gtcgtgctca
    16381 ccgaccgcaa cgtctacgtc atgcagaacg cgttcctgcg cacctacaag ttcaacggga
    16441 tcgcgcacaa ggcgccgctc gcatccgccc ggatcgattc gagcggcagc tgggtctcga
    16501 tcgacggcgg ccccaagctc cacgtgccgc cgttcggccc gatcaagcgc gggctcaccg
    16561 acttccgggc ggcgatcggc cagacttagg ggggcgaact ctccgccccc gcgcctgacc
    16621 ggtatgtgct ccccaagcgc gtcaggcgac ttccgtcgca tcacctgggc ggaggcctga
    16681 ccctcatcga ggcaagtggc atccgtgcgc ggctgctcgg gctctccctt ctgcccgggc
    16741 tgccgcgctg ccacgccctc ctcatcccgc gctgctcgtc ggtccacacc ttcgggatgc
    16801 gcttcgcgct cgacgtgctg ttcctcggcc cggagcacga gctcctgcgc gtcgagcgcg
    16861 acgtccggcc gaaccgcgtc ctcggctgcc gcaagtcgac ggcggtcatc gaggtccccg
    16921 ccggccaggc ggccccgttc gtgcgcgcga tccgcgccgg cgcgctcgcg cgcgaccgcg
    16981 gctagcccgc cgaccccacc agccgccgca gccgcacggt gagcgccgac agcgccagct
    17041 cctcggtcac gttcagagcc agcgacaggc gcgtctcctc gcagcgctcg accgcctggc
    17101 gcagccgccg cgcatcgcgc ccgcgcgccg ccggcgccag cgcctccacg cggtcgctcc
    17161 ccagcaccgc gccctcggcc cgctcggcca cgcacacgag atcgcggaac gccagcgcgc
    17221 acagctcgag ccccaggtcg agcacctcgg tgcgagcacg gcgcccgtcg cgcttggcgg
    17281 tgtcgtcgaa ctcgcgctcg atcgcgctgc ggtcgcggcc cttgggctcg tcggccagcc
    17341 gctccttgcc caaagcagcc acggcagcgg cagcctcgcg gccagccccc tccgcgcgct
    17401 ccaagagcgg cgcccacggc tccccggcgc gcacgtcgcg ctcctccccg gccagtgcat
    17461 ccaggaccat ccgctccacg tcctcccgca gcgcctcccc ctcggccgac gcgaggaacc
    17521 gcgcgcgcgt gaggttcccg agcgcgagcc gcccgcacgc gcccgcccgg cccggctcga
    17581 ccccctcgcc cgacaggacg gaggcgatcc gctccgccgg cagcgggtcg aagcgcacca
    17641 gctggcagcg cgagaccacc gtctcgagca cctgaccgag cgcgtccgtc agcaggatca
    17701 ggtgcacgta tgccggcggc tcctcgagcg tcttcagcat ccggttcgcc accgcgtcgc
    17761 tcatcgtgtc cgcgccctcg agcacgaaca cgcgcttgga ggactcgaac ggcgtgcgcg
    17821 tggccgcccc caccaccgcc tcgtccacgt cgctcacgcg catctcgtgc gcgccggtag
    17881 gccgcaccca ggtgagatcc gggtgcgttc ccgcgcgcac gcgcgcgcga gcgccgtcgc
    17941 catcggcctc cccggcggcg agcagctcgg ccgcgagcgc gcgtgccgcg gtgcgcttgc
    18001 cggtgccggt gggaccgtgg aacagatatg cgtgcgaggg catgccggag agcgccgcgc
    18061 ccagcaccat ccgcgcgtgc gggtggtcct cggtccccgg cagcgcgacg gccgtcacgc
    18121 cggcgcgccc gaggccagcg ccgcctcgac cgcggcgagc acgtccgcgt ggaccgcctc
    18181 cacgtcgcga tccgccgcca cccgccgcca ccggcccggc gccgcggccg cgagctgctc
    18241 gtaggcgccc gccacccggc gctggagcgc ctccccctcg tcctcgaacc gatcccgctc
    18301 cccggcgcgc gcggccgccg cctccgggtc caacgcgag
//
LOCUS       DASVPL010000200         4581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_259881, whole genome shotgun sequence.
ACCESSION   DASVPL010000200 DASVPL010000000
VERSION     DASVPL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259881"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VF517_10515"
     CDS             <1..1041
                     /locus_tag="VF517_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053415.1"
                     /translation="VPNDDHAPPVDDVDYSAYAEAFARRYGRGGEFWAAHPELPPLPV
                     TAYHVWNEPDLAHFWRPEPDAPRYARLYKAARAAIKRADPNAAVVLGGLSPLADGFVE
                     KLYAAEPSLRGNVDVLAYHPYGASPEHVLKQVRRMRATLDGLGETAVPLWITEIGWPT
                     QGRGGLPQAIPDRTRAGNVSLLTDTLLGSNCHVDVAAAYQWVAAERDPGFDEHWMGIY
                     HQDGTPTETGVAYGESVARNAALAGPHPALGLCTGRDRGGAAAKPLKLGLDVHATGSR
                     CFEAGATYRRRPVNGVEVTFAAGSIVKRTDTSTAGVARRCLPRAAAGRALRASARLLD
                     VAASATARRRVR"
     gene            complement(1054..2502)
                     /locus_tag="VF517_10520"
     CDS             complement(1054..2502)
                     /locus_tag="VF517_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053416.1"
                     /translation="MIKQVPSVPRIAAMALFAMSCVGILLFLWLQFGGPIPLRPEGYN
                     IEIAFPEATGLQKNLDVRASGITIGKVREVRVDHRTSRTLAKLAIDKEYAPLPSDMRA
                     ILRTKTLLGETFVELSQGSRTAPKIFDGGRLRDGRVAETVELDEIFQTYDPTTRKAIQ
                     LWQQELGRGVRGRGESLNNAVGNLPQFADSASDLLQVLYRQEGAVRGLFRDTGEVYEA
                     LTQDEDQLSNLIKNSHGLFSQTAQQRERLAEAFQIFPTFLDESRQTLERVEAFSRHAQ
                     PLVRDLQPVMRELRPTLVSLRSLAPSLDTYFRGFDRQIDVSGRSLPALREVVDETVPT
                     FRSLGPFLSEFNPIFEWLELHQHLVADFLGNGSAGTRDTVEGHGPGETGHYLRQLGPT
                     GAESLGIHPRRLSTNRGNAYLPPIIPDRDSVERKIFPNWDCLPANNFSDPAAPVDRAA
                     GPPARPACFVRRTFGWQGNQGRFLHVGPNDYR"
     gene            complement(2499..3812)
                     /locus_tag="VF517_10525"
     CDS             complement(2499..3812)
                     /locus_tag="VF517_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053417.1"
                     /translation="MMAVLRKNFLALLPLLALAAIGLVVGTYILANQRLRFPIVEDQP
                     IRMKVELPTAQAVTPGQGQFVRVAGVKIGSIGKVELEDGHASVVIEIDRKYEGLIRAD
                     ATMLLRPKTGLKDMFLEVDPGRGKPLEEGATLRLGNAIQDVNLDEILAMLDRDTREYL
                     LLLIHGAGKGLEGRGRDLREVFRLFEPTHRDLARLNSSVATRKVNLRRLVTALERLGS
                     ELAGREDELGQLVSSAARVFRAYASEDRNISAAVRELPKALKQTTRTVSEVERLAEVL
                     GPASEALRAPSRALGRYQRALRPLSIEAEPLLRKEIRPFVREARPTVRSLRPAALDLR
                     RATPNLTRSFVVLNRLFNMAAYNKDGREPPGDPDRDEGYLFYLGWLPHNSATTFATAD
                     AHGPFRPSLVAASCQTIRTLATERPESEYALNLTPLLTNPTLCGE"
     gene            complement(3809..>4581)
                     /locus_tag="VF517_10530"
     CDS             complement(3809..>4581)
                     /locus_tag="VF517_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053418.1"
                     /translation="LRAGRPALARLNEAFPPLRRLVADLRPSVRESGRTIGVSFPFIR
                     QTRRLVSRAELRGLVADLRPTVPNLARLTRDTVPLFERLRESAGCENEVFDHFANDRL
                     EDPAFPATGRIYEEAPKPLPSLSAESRNGDANGQWFHVLVSAGDYTVNIGDDVNGNPQ
                     FAQAYFPILGSQPPTPHGGRPPLHPEVPCETQQPPDLRSTAGPGDPVVARGLPNTRAA
                     RTRYERAKTRAVGFMRRDLKRMSLDKQFKVSSREIGRR"
BASE COUNT          603 a         1653 c         1682 g          643 t
ORIGIN      
        1 gtcccgaacg acgaccacgc cccgccggtg gacgacgtgg actacagcgc gtacgccgag
       61 gcgttcgccc gccgctacgg gcgcgggggc gagttctggg ccgcccaccc ggagctgccg
      121 cccctgcccg tgaccgccta ccacgtctgg aacgagccgg acctcgccca cttctggcgc
      181 cccgagccgg acgcgccgcg ctacgcccgc ctctacaagg cggcccgcgc cgcgatcaag
      241 cgcgccgacc cgaacgcggc cgtggtcctc ggcgggctct cccccctggc cgacggcttc
      301 gtcgagaagc tgtacgcggc cgagccgtcg ctgcgcggga acgtggacgt gctggcctac
      361 cacccgtatg gggcgagccc cgagcacgtg ctgaagcagg tgcggcgcat gcgcgcgacc
      421 ctcgacggcc tcggcgagac cgccgtgccg ctctggatca ccgagatcgg ctggcccacc
      481 caggggcgcg gcgggctgcc gcaggcgatt ccggaccgga cgcgggccgg gaacgtctcg
      541 ctcctcaccg acacgctgct cggctcgaac tgccacgtcg acgtggcggc cgcctaccag
      601 tgggtcgcgg ccgagcgcga cccgggcttc gacgagcact ggatgggcat ctatcaccag
      661 gacgggacgc cgaccgagac cggggtggcc tacggcgagt cggtcgcccg caatgccgcg
      721 ctcgccggcc cgcaccccgc gctcgggctc tgcaccggta gggaccgcgg cggggccgcc
      781 gcgaagccgc tgaagctggg gctcgacgtg cacgccacgg gctcgcgctg cttcgaggcg
      841 ggtgccacct accggcgccg cccggtgaac ggggtcgagg tcacgttcgc cgccggctcg
      901 atcgtcaagc gcaccgacac cagcaccgcc ggggtcgccc gccgctgcct cccccgcgcg
      961 gccgcggggc gcgcgctcag ggcctcggcg cggctgctcg acgtggccgc gtcggccacc
     1021 gcgcgccgcc gcgtgcgcta acgcctcccc ccgctagcgg tagtcgttcg gccccacgtg
     1081 caggaagcgg ccctggttgc cctgccagcc gaaggtgcgc cgcacgaagc acgccgggcg
     1141 cgccggcggg ccggccgctc gatcgacggg cgccgccggg tcgctgaagt tgttcgccgg
     1201 caggcagtcc cagttcggga agatcttgcg ctcgacggag tcccgatccg ggatgatcgg
     1261 cggcaggtac gcgttgccgc ggttggtcga caaccggcgc ggatggatgc ccagcgactc
     1321 ggcgccggtc gggccgagct ggcggaggta gtggccggtc tcgcccggcc cgtggccctc
     1381 gaccgtgtcc ctggtgccgg ccgagccgtt gccgaggaag tccgccacca ggtgctggtg
     1441 cagctcgagc cactcgaaga tcgggttgaa ctcgctcagg aacggcccga gcgacctgaa
     1501 cgtcggcacg gtctcgtcga cgacctcgcg cagcgcgggc agcgagcgcc cggacacgtc
     1561 gatctggcgg tcgaagccgc ggaagtaggt gtcgaggctc ggcgccaagg agcgcagcga
     1621 gacgagcgtg ggccgcagct cgcgcatcac cggctggagg tcgcgcacga gcggctgcgc
     1681 gtgccgcgag aacgcctcca cgcgctcgag ggtctgccgc gactcgtcca ggaacgtcgg
     1741 gaagatctgg aacgcctcgg ccagcctctc gcgctgctgt gccgtctgcg agaacagccc
     1801 gtgcgagttc ttgatcaggt tcgagagctg gtcctcgtcc tgggtgagcg cctcgtacac
     1861 ctcgcccgtg tcgcggaaga gcccgcgcac cgctccctcc tggcgataga gcacctggag
     1921 caggtccgag gcgctgtcgg cgaactgcgg caggttcccg accgcgttgt ttaggctctc
     1981 ccccctcccc cgtacgcctc ggccgagctc ctgctgccag agctggatcg ccttgcgcgt
     2041 ggtcgggtcg taggtctgga agatctcgtc gagctcgacg gtctcggcga cccgaccgtc
     2101 gcgcaggcgc ccgccgtcga agatcttcgg cgccgtccgc gagccctgtg agagctccac
     2161 gaacgtctct ccgagcagcg tcttcgtgcg cagtatcgcg cgcatgtcgc tcggcagcgg
     2221 cgcgtactcc ttgtcgatcg cgagcttcgc cagcgtgcgg ctcgtccggt ggtccacgcg
     2281 cacctcgcgc accttcccga tcgtgatccc ggacgcgcgc acgtcgaggt tcttctggag
     2341 cccggtcgcc tcggggaacg cgatctcgat gttgtagccc tcgggacgca gcgggatcgg
     2401 gccgccgaac tggagccaga ggaacagcag gatccccacg cacgacatgg cgaacagggc
     2461 catcgcggcg atccggggga cgctcgggac ctgcttgatc attcgccgca gagggtcggg
     2521 ttcgtgagca gcggcgtgag gttgagcgcg tactcggact cgggccgctc ggtggcgagc
     2581 gtgcggatcg tctggcagct cgcggcgacg agcgagggcc ggaacgggcc gtgcgcgtcg
     2641 gcggtggcga acgtcgtcgc ggagttgtgc ggcagccagc cgaggtagaa gaggtagccc
     2701 tcgtcgcggt cggggtcgcc cggcggctcg cggccgtcct tgttgtaggc ggccatgttg
     2761 aagagccggt tgagcacgac gaacgagcgg gtgaggttcg gggtcgcgcg ccgcaggtcg
     2821 agagccgccg ggcgcaggga gcggacggtg ggccgcgcct cgcggacgaa cgggcggatc
     2881 tccttgcgca ggagcggctc ggcctcgatc gacagcggcc gcagcgcgcg ctggtagcgg
     2941 ccgagcgcgc gcgagggcgc ccggagcgcc tcgctcgccg gccccagtac ctcggcgagc
     3001 cgctcgacct cggagaccgt gcgcgtggtc tgcttgagcg ccttcgggag ctcgcgcacc
     3061 gccgccgaga tgttgcggtc ctccgacgcg taggcccgga acacccgcgc agccgagctc
     3121 acgagctgcc cgagctcgtc ctcgcgcccg gcgagctcgc tgccgagccg ctcgagcgcg
     3181 gtcaccagcc ggcgcaggtt caccttgcgc gtggcgaccg acgagttcag gcgcgcgagg
     3241 tcgcggtgcg tgggctcgaa caggcggaac acctcgcgga ggtcgcgccc acggccctcg
     3301 aggcccttgc ccgccccgtg gatcagcagc agcaggtact cgcgcgtgtc gcggtcgagc
     3361 atcgcgagga tctcgtcgag gttcacgtcc tggatcgcgt tgccgagccg cagcgtggct
     3421 ccctcctcga gcggcttgcc gcggccggga tcgacctcga ggaacatgtc cttcagcccg
     3481 gtcttgggac gcagcagcat cgtcgcgtcc gcgcggatca ggccctcgta cttcctgtcg
     3541 atctcgatga ccacgctcgc gtggccgtcc tcgagctcga ccttgccgat cgacccgatc
     3601 ttcacgccgg ccacgcgcac gaactgcccc tgcccgggcg tgacggcctg ggcggtcggc
     3661 agctcgacct tcatccggat cggctggtcc tccacgatcg ggaagcgcag ccgctggttg
     3721 gcgaggatgt aggtgccgac cacgaggccg atcgcggcca gcgccagcag cggcagcagc
     3781 gccaggaagt tcttgcggag cacggccatc atcggcggcc gatctcccgc gagctgacct
     3841 tgaactgctt gtcgagactc atccgcttga ggtcgcggcg catgaagccc acggcgcggg
     3901 tcttcgcgcg ctcgtagcgc gtgcgcgccg cgcgcgtgtt cggcaggccc ctggcaacca
     3961 ccgggtcgcc gggccccgcg gtcgagcgta ggtcgggcgg ctgctgggtc tcgcacggca
     4021 cctccgggtg cagcggcggc cggccgccgt gcggcgtcgg cggctgcgag cccaggatcg
     4081 ggaagtacgc ctgcgcgaac tgcgggttgc cgttgacgtc gtcgccgatg ttcaccgtgt
     4141 agtcgccggc cgagacgagc acgtggaacc actgcccgtt cgcgtcgccg ttgcggctct
     4201 cggccgacag cgagggcagc ggcttgggag cctcctcgta gatgcgaccg gtggccggga
     4261 aggcggggtc ctcgagccgg tcgttcgcga agtggtcgaa cacctcgttc tcgcagccgg
     4321 cgctctcccg cagccgctcg aacagcggca cggtgtcgcg cgtgagccgc gcgaggttcg
     4381 gcaccgtcgg gcgtaggtcg gccaccagcc cgcgcagctc ggcccgcgag acgaggcgcc
     4441 gcgtctgccg gatgaacggg aagctcacgc cgatcgtgcg gcccgactcg cgtacgctcg
     4501 ggcgcaggtc ggccaccagc cgccgcaacg gcgggaacgc ctcgttcagc cgggccagcg
     4561 ccgggcgccc ggcgcgcagg g
//
LOCUS       DASVPL010000201         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_335806, whole genome shotgun sequence.
ACCESSION   DASVPL010000201 DASVPL010000000
VERSION     DASVPL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335806"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF517_10535"
     CDS             <1..316
                     /locus_tag="VF517_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053419.1"
                     /translation="ASGRVRRVAAGCRVQLALVRRAGGRCAFWRASRRRMEWRSCASP
                     VWTGAGETLREGDLTRWSHRFRASVAAGSYELRLRLVDRRGRVHVPAGRAALGFSLGP
                     PR"
     gene            374..1207
                     /locus_tag="VF517_10540"
     CDS             374..1207
                     /locus_tag="VF517_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX8053420.1"
                     /translation="MRAAAIQLNSDDDRPRNLRVADQLTRAAAADGADLIVLPEKFDL
                     IGTDAAMERGADTLDGPAIDWARSTARELGVDLVAGSIAERRPDRDKLSNTSVHIGPD
                     GEIAASYRKIHMFDVVVGGVEYRESATEEPGDELAVSHLADGTELGLSVCYDVRFPEL
                     FRILALRGARVIALPAAFTKVTGRAHWEVLVRARAIENQAFVVAADQCGVHPPGDKES
                     FGGSLIVDPWGEVLARAGDVPCFVAADLDLARQDEVREQLPSLANRVPSAYRWPAEVH
                     A"
     gene            1207..1845
                     /locus_tag="VF517_10545"
     CDS             1207..1845
                     /locus_tag="VF517_10545"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8053421.1"
                     /translation="MARGGNGGVDKRRLILDAAINVFARKGFHHCRVSDVADEAGVAY
                     GLVYHYFDSKEEILNTLFLERWQLMLDAIVGIDRQQDVPAREKLYMVASFIIDSYRHE
                     PELMKVIIVEVTRAANTFGRLHLSKIREAYTLIGDIVDSARQEGAFKADITPEFAAMC
                     FYGAIEQLLSSWIFDILPHSDEELENAKGLVVDAICGGLEVVPAPGLSSVSG"
     gene            1889..2011
                     /locus_tag="VF517_10550"
     CDS             1889..2011
                     /locus_tag="VF517_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053422.1"
                     /translation="MTKRLMWSGLLAGVGALTTIVANKLATVIWQKVFDEDPPE"
     gene            2008..2430
                     /locus_tag="VF517_10555"
     CDS             2008..2430
                     /locus_tag="VF517_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053423.1"
                     /translation="MSQEDNQDTRESPALDENEVQFTTADAEAVGDPAAAGTDAEGDP
                     GSAPVEAPGAGETAATATTGEPPQDEPAVDSEPTASVYEQPAGSPAEPPAGSPAAAQA
                     RANSGGDSPLERPEVQILGAFVGAFILAKLLQRLFGRD"
     gene            2430..2849
                     /locus_tag="VF517_10560"
     CDS             2430..2849
                     /locus_tag="VF517_10560"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX8053424.1"
                     /translation="MADNGVQPGGPADKQLGAIVADVTQKAQLLVREEIALAKAEITV
                     KTTKIVKGAVAFAAAGFFALMMLVFLLHMLSLGIADWFDLKTWVGYAATTLLLLIVTG
                     VSALVGVRFMKKGAPPVPEYAIEEAQKTRVAIEEARR"
     gene            2849..3070
                     /locus_tag="VF517_10565"
     CDS             2849..3070
                     /locus_tag="VF517_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053425.1"
                     /translation="MPERTPEQIRASVQETRRELEFSLNDLQAKVTELTDWRSQIAQH
                     RQQIVIGAAVAGFVIGGGIAAALGVFKRG"
     gene            complement(3253..>3651)
                     /locus_tag="VF517_10570"
     CDS             complement(3253..>3651)
                     /locus_tag="VF517_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX8053426.1"
                     /translation="RAELKMWFAIDTLEFLKRSGRIGAASAWIGSTLKIKPILTLEAE
                     ITPVERVRTSTRMFERMVDYARQREGSGANGWVVQHIQALEPANELADRCREIFGREP
                     VFFSEVGPVLGAHTGPGLLGVGSIPERFLV"
BASE COUNT          539 a         1295 c         1311 g          506 t
ORIGIN      
        1 cgcgagcggg cgcgtgcgcc gcgtcgccgc gggctgccgg gtgcagctcg cgctggtgcg
       61 cagggccggc gggcgctgcg cgttctggcg cgcgtcgcgg cggcggatgg agtggcgctc
      121 gtgcgcgagc ccggtctgga cgggcgcggg ggagacgctg cgcgagggcg acctgacccg
      181 ctggagccac cgcttccgag ccagcgtggc ggcggggagc tacgagctgc ggcttcgact
      241 cgtggaccgc cggggacgcg tgcacgtgcc ggccggtcgc gccgcgctcg gcttttcgct
      301 cggccctccc cggtagactg attggtcagt caacgccgcc agtccgaagc ggcgtgcggc
      361 aggggacgag tgcatgcggg cagctgcgat ccagctcaac tcagacgacg accggccgcg
      421 caacctccgc gtggccgacc agctcacacg tgcggccgcg gccgacggcg cggatctgat
      481 cgtcctcccg gagaagttcg acctgatcgg aaccgacgcc gcgatggagc gcggtgcgga
      541 cacgctcgac ggcccggcca tcgactgggc gcgctcgacc gcccgcgagc tcggcgtgga
      601 cctcgtggcc ggctcgatcg ccgagcgccg gcccgatcgc gacaagctct cgaacacctc
      661 ggtgcacatc gggcccgacg gcgagatcgc cgcgagctac cgcaagatcc acatgttcga
      721 cgtggtcgtg ggcggggtcg agtaccgcga gtcggccacc gaggagccgg gcgacgagct
      781 ggccgtgtcg cacctcgccg acggcacgga gctcggcctc agcgtctgtt acgacgtgcg
      841 cttcccggag ctgttccgga tcctcgcgct gcgcggcgcg cgcgtgatcg cgctcccggc
      901 cgccttcacg aaggtcaccg gccgcgctca ctgggaggtg ctggtgcggg cgcgcgcgat
      961 cgagaaccag gcgttcgtgg tcgccgccga ccagtgcggc gtgcacccgc ccggcgacaa
     1021 ggagagcttc ggcgggtcgc tgatcgtgga cccgtggggt gaggtgctgg cgcgcgcggg
     1081 cgacgtgccc tgcttcgtgg ccgccgacct cgacctggcc cgtcaggacg aggtgcgcga
     1141 gcagctcccg agcctggcca accgcgtgcc gagcgcctac cgctggcccg cggaggtgca
     1201 cgcctgatgg cccgcggcgg caacggcggt gtggacaagc gccgcctgat cctcgacgcg
     1261 gcgatcaacg tcttcgcgcg caagggcttt caccactgcc gcgtctccga cgtcgccgac
     1321 gaggcgggcg tggcgtacgg cctcgtctac cactacttcg attccaagga ggagatcctc
     1381 aacacgctct tcctcgagcg ctggcagctg atgctcgacg cgatcgtcgg gatcgaccgc
     1441 cagcaggacg tgcccgcgcg cgaaaagctc tacatggtcg cgtcgttcat catcgactcg
     1501 taccgccacg agccggagct gatgaaggtc atcatcgtcg aggtcacgcg tgcggccaac
     1561 accttcggcc ggctgcacct gagcaagatc cgcgaggcct acacgctgat cggcgacatc
     1621 gtcgactcgg ctcgccagga gggcgcgttc aaggccgaca tcacgcccga gttcgccgcg
     1681 atgtgcttct acggcgccat cgagcagctc ctctccagct ggatcttcga catcctcccg
     1741 cactccgacg aggagctgga gaacgccaag ggactggtcg tcgacgcgat ctgcggcggc
     1801 ctcgaggtgg tcccggcccc ggggctctcc tccgtgtctg gttagatacc gcgctgcccg
     1861 ggcaaccgga ctggatatgg gcaacgacat gacaaagcgc ctgatgtgga gcggcctgct
     1921 cgccggagtg ggtgctctga ccacgatcgt ggccaacaag ctcgcgaccg tgatctggca
     1981 gaaggtcttc gacgaggatc caccggagtg agccaggaag acaaccagga cacgcgcgag
     2041 agcccggctc tcgacgagaa cgaggttcag ttcacgaccg ccgacgccga ggccgtgggc
     2101 gacccggccg ccgccgggac cgacgccgag ggcgacccgg gcagcgcgcc ggtggaggcg
     2161 cccggagcgg gcgagaccgc ggccacggcc acgaccggcg agccgccgca ggacgagccc
     2221 gccgtcgaca gcgagccgac cgcgagcgtg tacgagcagc ccgccggatc gccggccgag
     2281 ccccccgccg gatcgcccgc cgccgcccag gcgcgtgcga acagcggcgg cgacagcccg
     2341 ctcgagcggc ccgaggtgca gatcctcggc gcgttcgtgg gcgccttcat cctcgcgaag
     2401 ctgctccagc ggctgttcgg cagggactga tggccgacaa cggggtccag ccgggcggtc
     2461 cggccgacaa gcagctcggc gcgatcgtcg ccgacgtcac gcagaaggcg cagctcctcg
     2521 tgcgcgagga gatcgcgctc gcgaaggccg agatcaccgt caagacgacg aagatcgtca
     2581 agggggccgt ggcgttcgcg gccgccggct tcttcgcgct gatgatgctg gtgttcctgc
     2641 tccacatgct gtcgctcggc atcgcggact ggttcgatct gaagacgtgg gtcggctacg
     2701 cggccaccac gctcctgctc ctgatcgtca ccggggtctc ggccctagtc ggggtgcgct
     2761 tcatgaagaa gggtgcgccg ccggtgccgg agtacgcgat cgaggaggcc cagaagacac
     2821 gggtcgccat cgaggaggcg cgccgctgat gcccgagcgc acccccgagc agatccgcgc
     2881 ctcggtccag gagacccgcc gcgagctcga gttctcgctg aacgatctcc aggcgaaggt
     2941 caccgaactg accgactggc gcagccagat cgcccagcac cgccagcaga tcgtgatcgg
     3001 agcggccgtc gccggcttcg tgatcggcgg cgggatcgcc gccgcgctcg gcgtcttcaa
     3061 gcgcgggtag ggcgctcacc gcgctgcgcc gcgccgcgcc gcggggctgg cgcccactgc
     3121 cgctgcgttc gcgcggctgc cggcaacggc ggctggggag gggggagagg gtctcgatcg
     3181 tcgattggag cctttggtcg catatagcca cctaaccctc caatcgatga gcgcgggcct
     3241 cgtccgccgc cgctagacca ggaaccgctc ggggatcgag ccgacgccca ggagcccggg
     3301 gccggtgtgg gcgccgagca cggggccgac ctccgagaag aagaccggct cgcggccgaa
     3361 gatctcgcgg cagcggtcgg ccagctcgtt ggcgggctcg agcgcctgga tgtgctgcac
     3421 gacccagccg ttcgcgcccg acccctcgcg ctggcgcgcg tagtccacca tccgctcgaa
     3481 catccgcgtg ctcgtccgca cgcgctcgac cggcgtgatc tccgcctcga gcgtgaggat
     3541 cggcttgatc ttcagcgtcg acccgatcca cgcgctggcg gccccgatcc gcccgctgcg
     3601 cttgaggaac tcgagcgtgt cgatcgcgaa ccacatcttc agctccgcgc g
//
LOCUS       DASVPL010000202        27182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_63237, whole genome shotgun sequence.
ACCESSION   DASVPL010000202 DASVPL010000000
VERSION     DASVPL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 27182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27182
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63237"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VF517_10575"
     CDS             <1..659
                     /locus_tag="VF517_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053427.1"
                     /translation="PHSEPPRRNNTPLIAAALAIVAIAGVIVALLATGTLGGDNGGDE
                     PATSEASSTATTTTERTTDEEPETVTRTETAPAPPAATEFESFTSEAGDFEALLPAGA
                     DWSEPDVSTVGEGIVRTRLTGPDGLEVIIDHTPAEAATFKPADRCNQTSLPTVPYAAK
                     CVFQGGSLEPCQRARCVDYLMNAGVDGPGWGVLVGGGDFAETERIAKRIATTLTPLDR
                     "
     gene            659..1678
                     /locus_tag="VF517_10580"
     CDS             659..1678
                     /locus_tag="VF517_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX8053428.1"
                     /translation="MAPPVHSGASVELPGAEPLPLERLARRFALAVGLILFVAALSYL
                     GRDGYADAAGGKIGFLDALYYSTVTITTTGYGDIRPESDEARLVTTLLITPARILFLI
                     LLVGTTVELLAEHTRRAYRLGRWRRGLQDHIVICGFGTKGRAAVSTLEGRGVPAKEVV
                     VIDERPDTLEQARALGFATVAGNASRSEVLEAAGIRRAKAIVVAADRDDAAVLITLTA
                     RELNRTATIASAVREEENAHLLRESGADSVITSSGAAGRLLGMATQSPQLVEVLEDLL
                     AVGSGLDIVEREVRPDQVGPLGAHGSREPIVALVRDGELIRFDDDRAAELQLGDRIVC
                     LCNAG"
     gene            complement(1675..2085)
                     /locus_tag="VF517_10585"
     CDS             complement(1675..2085)
                     /locus_tag="VF517_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053429.1"
                     /translation="MKRLRRPGGMDPPAVATLPDGEAIELVAAAEEVTRRYFAEFPDD
                     LDNYSAEVRDWAVHDTQHILSWAVSELNGFRALGRQVDWLARVLAARDFPLERLARNL
                     ELSADVLAERVPGERDAIEAMFAEAARGVRAASA"
     gene            complement(2082..2390)
                     /locus_tag="VF517_10590"
     CDS             complement(2082..2390)
                     /locus_tag="VF517_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX8053430.1"
                     /translation="MYVSMSHLRVDAGRAGELVEAFRNRIGLVDAADGFVDLEVWQAD
                     SDPGELIMVSRWRDRDAFKRYMKSAEHQHSHERIDPGLQEAIKLTGLGHLHTYEVVAR
                     "
     gene            complement(2409..3209)
                     /locus_tag="VF517_10595"
     CDS             complement(2409..3209)
                     /locus_tag="VF517_10595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8053431.1"
                     /translation="MSREAWILAVVGVLGALTLGGASFARDDAREAPTFDRPLFAGSF
                     EADGAATERGARPWHSGARLRGGAGSRVAIRSGRALRLRERPGGRTLAVLGARTPFGS
                     AMVLPVVRRRAGWLGVLASALPNGRVGWIRDDGSALTHARTRVRVLVDRSDRRLVLVR
                     DGREVASAEVGVGRPGAETPIGHFAVTDKLDGGRFARGYGCCILALSGRQPNLPAGWR
                     GGDRLAIHGTNGASASRVRSAGCVALDEAPLRRLMETVPLGTRVTIRP"
     gene            3315..3764
                     /locus_tag="VF517_10600"
     CDS             3315..3764
                     /locus_tag="VF517_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8053432.1"
                     /translation="MSERGTTAEAALAPDLVDGICELTLEADDLDLLAGFYNEAFGFR
                     TIKRDDDRAWLACGSRTRLGIWSPGPKEFGDRGGRHVHFALSAAPEALDAVAARLRSR
                     SVGFQGPVEHPGGDRSIYVQDPEGNVVEVWSFFQQDAGAIDGVDALA"
     gene            complement(4023..5264)
                     /locus_tag="VF517_10605"
                     /pseudo
     CDS             complement(4023..5264)
                     /locus_tag="VF517_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5404..6009
                     /locus_tag="VF517_10610"
     CDS             5404..6009
                     /locus_tag="VF517_10610"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8053433.1"
                     /translation="MPKVTQEHLDARRRQILDAALTCFAREGFHRTTMQDIFGEAGLS
                     PGAVYSYFKSKDEIIDAITREAARFAETGGTMIRENAEELTLAGAVAALAHGFEQLDV
                     GTAETRARMVPQLWVEAQRNSIVRDRARVGIGGAVEGLTEVARAAQERGDLDPELDPE
                     AAARVAISVFQGLLVQRSIFGADVDVDAYVEAALLMLGPRA"
     gene            complement(6006..6719)
                     /locus_tag="VF517_10615"
     CDS             complement(6006..6719)
                     /locus_tag="VF517_10615"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX8053434.1"
                     /translation="MKRVGHKGAGLIAPGNTVASFDAALDAGVDMIEFDVLPRDLRDP
                     ANTELVLSHDYEHLAEGPVLTFEEGLDHLAGETFATMHFNVDLKARGYELRVVEALRE
                     RGLVDRVLISTHHLESLDRIRAAEPRLKLGLSVPNVRRDYTQSPLLVLPALGALQVAR
                     LLVPARAGRLIRSGRCDALMAHWRFVTARLVRAVHAAGGELYVWTVDEEPAIRRLEAL
                     GVDAVISNDPRLFGERVPG"
     gene            complement(6726..7127)
                     /locus_tag="VF517_10620"
     CDS             complement(6726..7127)
                     /locus_tag="VF517_10620"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEX8053435.1"
                     /translation="MPARAPFADFFVSHHIGDYLLQTDFQALNKKGGLGEDEDSRTAL
                     LHHGMTYTAAFVPALLGVARRTSLTRAVGVAALIALPHMAIDDGRVVNAYMTRVKRTP
                     ADVDQKLTASVDQAMHMVCLWAAAFVARDRD"
     gene            complement(7154..9643)
                     /locus_tag="VF517_10625"
     CDS             complement(7154..9643)
                     /locus_tag="VF517_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX8053436.1"
                     /translation="MDRSPARTLVAALALAVALAAPAAAAETVPAGAIEARIDLDPWH
                     VEFVQPGGPTLAEAHATGTGPAGTIGFRTSTGWWHATRALTSAGDGRALTAELATTDP
                     LGRRLAVRIEPSGDGAIGVTAAVTGAAGTGDVEAVGVGFDAADRERYLGFGERSNAVD
                     QRGNEVESYVAEGPYQPEERAFLTAFVPPWGYHPRDDATYFPMPWLLSTRGYGVLIDN
                     NEASMHRLGSELSDAWSMETQASQIAFRVLAGPTPAATVKRLTERTGRQPKPLAPWFL
                     GPWFHTGQENVPPRAREEEAVRTLREADAPASAVETHLRYLPCGEAVPHRAAERERTD
                     FFHRNGLAALSYVNSEICQEYEPVWSDGAARGVFQKDSSGKPYVFNAYVGSRTPPQTP
                     VSQVDFTAPGAQDFWNGLMSQLVEDGHDGFMEDFGEYTPLDSQHANGMTGAQMHNYYP
                     VPYHRAGWEFARRQARPIARHVRSGWTGVHPYAQIVWGGDPTTDWGYDGLASAVTNAL
                     TMGLSGISTWGSDIGGYFSLGTRELTPELLIRWIQFGAVSGVMRTKFAGTAIPPRGER
                     PQPWSREIVGHWRRYAKLRTQLYPYIAAHEAEYRRTGLPLMRHLALTYPADARAAATD
                     DEFLFGPDLLAAPVLEPGATQRRLYVPNGRWFDLWRTLAYHEPTGGLKLGRPARLRPG
                     DRTVDAPLDQLPLLLRAGAVLPLLAPDVDTLADYGAGSGQVRLRDRSGRLDLIALPRG
                     RSESRMYERERLVSIEGSRGWRLKVEGRARRTYRLQASLATLRRPFRPCSVRIGSRRL
                     PRGAWSYRVTTGVLRATFTAKRGSLSVGRCG"
     gene            complement(9731..15040)
                     /locus_tag="VF517_10630"
     CDS             complement(9731..15040)
                     /locus_tag="VF517_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053437.1"
                     /translation="MIWSNRIRTLLAALVVGLAVLPAASASADRAYSLRYGQVERGDI
                     TTAANTIVTCPEGAPNCANARNGTGTVLRNSAWVMVPIDIDSDSTTFDSSSADLRLPA
                     GATILYAGLYWGSDTKAMPEGVDAPNPAARGTMRLAAPDGAGYRTITASTVDSDRFFP
                     SRYQGFADVTSLVRQAGAGTYTAANVQASTGKDQYGGWGLVVAYRDPSKPVRWLGLYD
                     GYRDFGGDDAADVPITGFRTPPSGTVDADFGMLAYEGDLGVQPDTARINGRDLVDPLH
                     ALNNYWNASITEYGSHVTTRNPSYINTLGMDANIMGIDGFLVNDATSAILHMEVATDL
                     VMPGAMTLVSDQAASAPANTAAPTVSGTARDGQTLTAGNGTWSGTTPMTFSYQWRRCD
                     SAGAACVDIDGATGSTYVVGAGDVGKTIRVQVTARNVVSSGVASSGATGVVVARAPVN
                     NAPPVVSGTARDGQALSTTNGSWTGTPTISFAYQWRRCDSAGNACVDIAGATGSSYTL
                     VPADVGKTIRVVVTASNAGGTAASTSDATGAVTARPPANGAPPVVSGTARDGQSLTTT
                     TGSWTGTPTISFAYQWQRCDSGGDACVDIAGATGSSYTLVPADVGKTLRAEVTASNAG
                     GSAASTSDPTGTVTARPPANSAPPVVSGTARDGQALSTTNGSWTGTPTISFGYQWRRC
                     DSAGGSCVDIAGATGSSYTLVPADVGKTIRVVVTASNAGGSAAATSDATGAVAARAPV
                     NNAPPVVSGTARDGQSLTTTTGSWTGTPTIAFAYQWRRCDSAGDVCTDIAGATGSSYA
                     LVPADVGKTIRVVVTASNAGGSATSTSDPTGTVAARAPVNNAPPVVSGTARDGQSLTT
                     TNGSWSGTPTISFGYQWRRCDSAGGSCVDIAGATGSTYALVPADVGKTLRAEVTGTNG
                     GGSASAVSAPSAVVAARPPTNNAPPVISGTARDGQTLSTTNGSWTGTPTISFTYQWRR
                     CDSAGNTCVDIAGATGSSYALVPADVGKTLRAVVTASNAGGSAASTSDATGAVAARAP
                     VNNAAPVVSGTARDGQSLTTTNGSWTGTPTISFGYQWQRCDSGGDACVDIAGATGTSY
                     TLVPADVGKTLRAEVTASNAGGSAASTSDPTGTVTARPPANNAPPVVSGTARDGQSLT
                     ATTGSWTGTPTITFAYQWRRCDSAGNGCASISGATGAAYELTPADVGHAIRVVVTATN
                     AGGSAASTSDPTGAVTASPPVNTAPPVVSGNARDSQTLTTTTGSWTGTPTISFAYQWR
                     RCDSAGNGCANISGATGASYELTPVDVGHAIRVVVTATNAGGSAALTSDPTGAVGVGP
                     PLNSTLPKISGTARDGLTLTADDGSWTGTPTITYTRRWRRCDAGGNDCANIVGATGTT
                     YTLTASDVGATIRVLVTAANGGGERSAESAPTAVVQAIPPANTTAPTLTGTAREGEEL
                     ALSDGSWTGSAPLTFTYAWERCGVDGSGCVPIAGATAASYTLVLADVGARVRGVVTAA
                     NAGGSRSAATDPTAVVTAKPVPPPPPPADSGGTTPGGSTGGTTPGGSTPGTTEPGTTP
                     AGSTPGGSTPGGSTPGTEPGTTPGGTTPDCGSTTGGSTTSGSTTPGGSTTGGSGTTSG
                     DCGTAPSAALGASQWKSSTGTGLRLRVDSDEPIKTLAFTAPRAMLPQRGDAGRVAGRI
                     TVYRKGAKSRTWTVRVTRAGGLATRGASAPRLALGRGTLRVSGLPAGAVSVKLTLYTR
                     NATSPKALVKPGHAARLDASLRTTAGRLEHLTYRLGRRTDRHS"
     gene            complement(15116..16585)
                     /locus_tag="VF517_10635"
     CDS             complement(15116..16585)
                     /locus_tag="VF517_10635"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8053438.1"
                     /translation="MGNGSRRQGPSVAIVGAGFGGIGLGIKLQQAGIESFTIFEKAGA
                     VGGVWRDNSYPGLTCDVPSHLYQFSFEPNHDWTRRFPQRDEILAYLERLTDKHGLREH
                     MRLGTEVAAADFDAERGRWVLRLVGGEEAEFDVLVTATGQLSRPSFPRIPGLDDFRGE
                     LFHSARWNHDYDMAGKRVAAIGTGASAVQYVPEVAKVVERLHVFQRTPGWVIPKPDRP
                     YRPREKALFRRIPLIPAISRRLTFIRFELFTLAMTRAYWMLRLYELGYKRRLRTEIPD
                     PQLREKLVPGYPMGCKRVLISNDYLPALARPNVEVVTDDIHEVTATGVATADGRVREV
                     DAIVLGTGFQANEFLAPMAIRGRDGADLNDVWRDGAEAYLGLAISGFPNMFMLYGPNT
                     NLGAGSIITMLESQIGFVVEAVRDLGSSGARWMDVRGDVQSAFNREVQERLGDSVWAA
                     GCNSWYRTESGRVTNNWAGFVTEYKRRTRRPDPADFVLA"
     gene            complement(16616..16834)
                     /locus_tag="VF517_10640"
     CDS             complement(16616..16834)
                     /locus_tag="VF517_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053439.1"
                     /translation="MRGTAVVQILIVLAVAVGLGYLVLDAVDGWEDWVVFGVVISAVV
                     GGAIAVWNRQYPNQSKKRVFTRDSDSSW"
     gene            16908..17342
                     /locus_tag="VF517_10645"
     CDS             16908..17342
                     /locus_tag="VF517_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8053440.1"
                     /translation="MVKTIAVGTDGSDTAKVAVDFALDMAERYGAKVVVASAYKPVSE
                     SQLRREQAEAPEDIQWSINPSEEVDTTLRAVEEEAKERGLSYASEARNGDPAEVLCDI
                     AEAHGADVIVVGNRGMQRRLLGSVPNSVAHKAPCSVMVVKTT"
     gene            complement(17339..17947)
                     /locus_tag="VF517_10650"
     CDS             complement(17339..17947)
                     /locus_tag="VF517_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX8053441.1"
                     /translation="MDLVSTLISSGWASGVNAYLTVALMSLLGRAGWGDVPEELQGNG
                     VLAAALVLYAIEFVVDKVPLLDSAWDVLHTIVRPAIASAVGVALGGDAGATGVEEVFA
                     GGGAGAMAFASHAVKAGIRLGVNSSPEPASNIAVSLLEDGLVAGVTVLAIEHPVPAAI
                     VAVILLVSGIALATFLVKRIRKAWRALRARWGGGGGPPALAR"
     gene            complement(17948..19150)
                     /locus_tag="VF517_10655"
     CDS             complement(17948..19150)
                     /locus_tag="VF517_10655"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8053442.1"
                     /translation="MSPGVGGSVVPGYEAVRYAFERNLSEGRELGAACAAYVGGEKVV
                     DLWGGVRDARSGARWDEDTLVIVYSTSKGLAAMTLALLHTRGLLDYDERVSTYWPEFG
                     QAGKERVTVRQLLGHEAGLPVVDEPLTPTLIADFDRLAEVIARQRPVWEPGTRHGYHG
                     LSLGWYEGELVRRIDPDHRTLGRFFAEEIAAPLGLDVHFGVPEDVPKRRIARIERAPI
                     RSLPGLRDMPPRMALAMANPRSLTFKAFANPRVYSPASFDSRRYRHLEFPAGGALGTA
                     RSVARAYMAFAARPPELGLSDATLAELTATPRPPARGWHDEVLKIDTAFSLGFARPRP
                     GELDFASPRAFGHPGAGGSFAFADPENEVSFAYVMNRMGYHIANDPREKALRDALYRC
                     LERERRAA"
     gene            complement(19147..19923)
                     /locus_tag="VF517_10660"
     CDS             complement(19147..19923)
                     /locus_tag="VF517_10660"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8053443.1"
                     /translation="MAIYEREGDAFVPTELARGPWDPRAQHGGAPAALLARSVELEAG
                     ELVVARLTYEFLRPVPLEPLSIATRVVRPGRRAQLIESTLETTAGPVCRVLGLVLARV
                     EGTAPAVPAHEPALPPPDGVAPSSSPPGERPMFAGDGVELRFVRGSLTEPGPAAAWVR
                     FLVPLVDGEEPSAVQRAAAAADFGNGVSASLDWNTHVFINADLTIHLLRPPRGDRVGL
                     DSRTVVDPDGVGMAESVLHDEHGLIGRAAQSLLVGRREAA"
     gene            complement(19929..20570)
                     /locus_tag="VF517_10665"
     CDS             complement(19929..20570)
                     /locus_tag="VF517_10665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8053444.1"
                     /translation="MPEVNPPEIDRYCEEHSTAPPGWLERLAAETREHLEFPQMLTGR
                     VEGRFLEMLVWASGARAVLEIGTYSGYSALSMAAALPDGGRIVSCEVSEKHAEFARRH
                     IEAAGYADRIEVRLGPALETIAELDGPFDLVFVDADKTGYSAYVDAVLPKLAPRGLIV
                     IDNTLWSGQVLDPDPDDESGTALAALNDRLAADERLVVVQLPVRDGVTLVRRA"
     gene            20608..21465
                     /locus_tag="VF517_10670"
     CDS             20608..21465
                     /locus_tag="VF517_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053445.1"
                     /translation="MRRALLVAVVLALVAGDGVAAAATAKLPRGGTTLFPRQRVVAFA
                     GTPGAPALGALGVGRLDHAVRRLRRQARHYHRAGRPILPALDLLATICSAGPGRDGKY
                     RYRRPLKIVQRHLAAARRARALLLIHIQPGRSTFMEELRPYRRFLSEPDVGVALDPEW
                     RMGPGQRPGKVIGSADSDEINAVSAYLDRVVKRHDLPQKLLVAHQFTPNMIRRRARVR
                     QRPGVAVTISIDGVGSRRLKEGTYSRLARSGDNLYDGFKLFYEEDGRVMTPAQVLRLR
                     PQPDFVVYE"
     gene            complement(21404..21961)
                     /locus_tag="VF517_10675"
     CDS             complement(21404..21961)
                     /locus_tag="VF517_10675"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8053446.1"
                     /translation="MAAVMWVLEIADATVGTGLDDYGIEPREPDGLVGIVASPFLHAD
                     FSHLMSNTVPFLVMGFAIALAGLVRVLSVTAIVMAVGGVGTWLVAPEHTNHIGASGVV
                     FGYAAYLLVRGFYNRNAFELFMGLIVGALWGSVLLSGLVPRAGISWQGHLFGGIGGVV
                     AAWLLATRTRRSRAAAEVSAPGRAS"
     gene            22099..22368
                     /locus_tag="VF517_10680"
     CDS             22099..22368
                     /locus_tag="VF517_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053447.1"
                     /translation="MGEQESGPRVNPEDVAKRQPPLAKVMEALAEPEGKTRRDSPTAT
                     ARDIALLMEARDASSPLASALDELERAHGGKASEDDLKPHLDPSD"
     gene            22397..23983
                     /locus_tag="VF517_10685"
     CDS             22397..23983
                     /locus_tag="VF517_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8053448.1"
                     /translation="MTPRAAVRAALVNPPHVRGPGRPLENASPPLGIAYIAAALRAGG
                     FPAELYDLADEVPMDPAKLAAAGFFDHDLYGFTSYTKTFDAALELVRLLKANNPAATI
                     VFGGPHATPLAEVLVETYDEIDVVVRNEGEQAMVAIARHRAGDLDSLAGVAGLVYRKG
                     GAIARTGDPHDLPPVDSLPDPARDYAIRPQRLVYDRRDPLNPQRIEFFSSSRGCPKRC
                     TFCSIVVMSPRYRFRSVESLMDEIRAVHERDPFGHVAFLDANFFVHWRRTAEFAEALH
                     RWDPGVTWSATATVDAIVRHPRVVERIGALNCQMLEVGVENGSDSFLARVNKRTTVEQ
                     NLDAIRALDDAGIELELDYIMFDPETTLEELADNADFLTEAELTDYIPADHLANTLRL
                     YPGTPARSRYAPGGPGPAEFAAADLYAPIADPRAAAVNRVMTEAVDTCFVGLEQVVRA
                     CERAADTSHDPQEMHALAIAFRNVPFAIFRESIDRAGAGELDLTLSDLAASARLRELV
                     ALAHEQETRASRPAELANAV"
     gene            23973..24302
                     /locus_tag="VF517_10690"
     CDS             23973..24302
                     /locus_tag="VF517_10690"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX8053449.1"
                     /translation="MPSSSPAPGVGGEAAASAFRLRPNPEVRVVEEHGTVYLIPPVGR
                     PLRLNRSASGLWRMLAEGRPRDEVVDRHAVDSGCDVATARDEIEQLIASLCENHYLMR
                     APADAPS"
     gene            24289..25980
                     /locus_tag="VF517_10695"
     CDS             24289..25980
                     /locus_tag="VF517_10695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8053450.1"
                     /translation="MRPRDRILTTGTRFERVPPNPAQPRLRVALINPPDPTSSDDGRS
                     FPALGLGYVAAFARVRGFHVDLFDFALDLDPDLDFLDRVGALDGYDVYGVSTYSDTLP
                     SALTVAAALRRGAPRSPIVFGGYHAAVLDAELLEDFDMIDYVVRDEGEIPFSELLDGL
                     ASDRSDHGSVASLTWRGADGAVVRNPSSERLLDQAELPFPVTETRHGPRRYADYADRD
                     SGERRRAVSLVSSRGCPKRCTFCSIALVNPLWRARSAESIMAEVRYRYAHEPFAHVFF
                     QDGNFFASPKRSLAFARELHAFDPSITWSGTATPDNVVRHAGVLAELAALNCASLELG
                     IESGNEGSLERFGKGTDRAVNLQAIRLLESAGIRLGLDFIMFEPEMTLDDVRANLDFL
                     HEADLYGYWPAECLFQELRLYPGTKMRTRHVPGDCTRHEVPPTPLRDAGLRTAFEEAW
                     AYRRTLQAATDEALKQLEVAIAGLDAVDRDGEEPEIRRLRQDAQACVIELRHAPYAFL
                     EAVASPSQAARPDELEAARTATRWALAGAVATVRSVRAAIGTWTAPARTRAERVA"
     gene            25977..26867
                     /locus_tag="VF517_10700"
     CDS             25977..26867
                     /locus_tag="VF517_10700"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8053451.1"
                     /translation="MSVSAEFLAARWQTAPEALPGLAADWLDRDWVARFESWAVPPTT
                     ARLFVLQEDGPAGAATAATMPDPRVAYELFQRYAGEGQQLTLLLNHTERVAPELRELR
                     DGLGIGNPWRHDDVVATLSTAGSGIGYHAGHEDSLVVQLAGRRHWRAWSADHVPASHR
                     HELVWRHAPEGPGAIRRPAADPLVDCELGPGDVLYVPSLFPHEGRTLELSVSLSIAWR
                     GVSVATLLDEVEALPAAERTLTERHPDAFLRLVRDPPPGAPDACAAVMEQLDPALRAL
                     RVSATEREWVRRSVDELLAG"
     gene            complement(26904..27146)
                     /locus_tag="VF517_10705"
     CDS             complement(26904..27146)
                     /locus_tag="VF517_10705"
                     /inference="COORDINATES: protein motif:HMM:NF039758.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6411 family protein"
                     /protein_id="HEX8053452.1"
                     /translation="MTIAAIVAVCVVLLVLAFLLPRLSRHPQRGVDRTLGVGQSAAGS
                     APGFLGRWLQKPFGSSRRATNKSASLGRRARGKMPL"
BASE COUNT         3726 a         9901 c         9939 g         3616 t
ORIGIN      
        1 cgccccactc cgagccaccg cgccgcaaca acacccccct catcgctgcc gccctcgcga
       61 tcgtcgcgat cgccggggtg atcgtggccc tgctcgccac cgggacgctc ggtggcgaca
      121 acgggggcga cgagcccgcc accagcgagg cctcctccac cgccacgacc acgaccgagc
      181 gcaccaccga cgaggagccc gagaccgtca cccgtacgga gaccgcgccg gcgccgccgg
      241 ccgccaccga gttcgagtcc ttcacgagcg aggccggcga cttcgaggcc ctcctgcccg
      301 cgggcgcgga ctggtcggag cccgacgtga gcaccgtcgg cgagggcatc gtccgcaccc
      361 ggctcaccgg ccccgacggt ctcgaggtga tcatcgacca cacccccgcc gaggccgcca
      421 ccttcaagcc ggccgaccgc tgcaaccaga ccagcctgcc cacggtcccc tacgccgcga
      481 agtgcgtctt ccagggcggc tcgctcgagc cctgccagcg tgcgcgctgc gtcgactacc
      541 tgatgaacgc gggcgtggac ggccccggct ggggcgtgct ggtgggcggc ggcgacttcg
      601 ccgagaccga gcggatcgcg aagcggatcg ccaccacgct gactccgctc gaccgctgat
      661 ggcgccaccc gtgcacagcg gggcgagcgt cgagctcccg ggtgccgagc cgcttccgct
      721 cgagcgcctg gcccggcgct tcgcgctcgc cgtcggcttg atcctgttcg tcgccgcgct
      781 ctcgtacctc ggccgcgacg ggtacgccga cgcggccggc gggaagatcg gcttcctcga
      841 cgcgctctac tactccacgg tcaccatcac gaccacgggc tacggcgaca tccggccgga
      901 gagcgacgag gcccggctgg tgacgacgct gctcatcacc ccggcgcgca tcctgtttct
      961 gatactgctg gtcggcacga ccgtcgagct gctggcggag cacacccgga gggcgtaccg
     1021 gctcgggcgc tggaggagag gcttgcagga ccacatcgtc atatgcgggt tcggcaccaa
     1081 ggggcgcgcg gcggtgagca cgctcgaggg tcgcggcgtg cccgcgaagg aggtcgtggt
     1141 gatcgacgag cggccggaca cgctcgagca ggcacgggcg ctcgggttcg cgactgtggc
     1201 cgggaacgcc tcgcgctcgg aggtgctcga ggccgcgggg atccggcgcg cgaaggcgat
     1261 cgtggtcgcc gccgatcgcg acgacgcggc cgtgctgatc acacttaccg cgcgcgagct
     1321 caaccgcacg gccacgatcg cctcggcggt gcgcgaggag gagaacgcgc acctgctgcg
     1381 cgagagcggc gcggactcgg tcatcacctc gtcgggcgcg gcgggccggc tgctcgggat
     1441 ggcgactcag tcgccgcagc tcgtggaggt gctggaggac ctgctggcgg tgggcagcgg
     1501 gctcgacatc gtggagcgcg aggtccgtcc cgaccaggtc gggccgctgg gcgcgcacgg
     1561 ctcgcgcgag ccgatcgtgg cgctcgtccg cgacggcgag ttgatccgct tcgacgacga
     1621 ccgcgcggcc gagctccagc tcggcgaccg gatcgtctgc ctctgcaacg ccggctaggc
     1681 gctcgccgcg cgcacgccgc gcgccgcctc ggcgaacatc gcctcgatcg cgtcgcgctc
     1741 cccgggcacg cgctcggcga gcacgtcggc cgacagctcg aggttgcgcg ccagccgctc
     1801 gagcgggaag tcgcgcgccg cgagcacgcg cgcgagccag tccacctggc ggccgagcgc
     1861 gcggaagccg ttcagctcgc tcaccgccca gctcaggatg tgctgcgtgt cgtgcacggc
     1921 ccagtcccgc acctcggcgg agtagttgtc gaggtcgtct gggaactcgg cgaagtagcg
     1981 ccgcgtgacc tcctcggccg cggccaccag ttcgatcgcc tcgccatccg ggagcgtcgc
     2041 cacggcgggc gggtccatgc cgcccggacg ccgcagccgc ttcaccgcgc gaccacctcg
     2101 taggtgtgca ggtggccgag gccggtgagc ttgatcgcct cctggagccc cgggtcgatg
     2161 cgctcgtgcg agtgttggtg ctcggcgctc ttcatgtagc gcttgaacgc gtcgcggtcg
     2221 cgccagcggc tgaccatgat cagctcgccg ggatcgctgt cggcctgcca gacctcgagg
     2281 tcgacgaacc cgtccgccgc gtcgaccagc ccgatccggt tgcggaacgc ctcgaccagc
     2341 tcgccggcgc gtcccgcgtc cacccggagg tgcgacatcg acacgtacat cgcctcgaac
     2401 cgtaaccact aggggcgaat cgtgaccctc gtgccgagcg ggacggtctc catcagccgc
     2461 cgcagcgggg cctcgtcgag cgccacgcag ccggccgagc ggacgcgcga ggcactggca
     2521 ccgttcgtcc cgtggatcgc gagccggtcg ccgccgcgcc agcccgccgg caggttcggc
     2581 tggcgtccgg acagggcgag gatgcagcat ccgtaaccgc gcgcgaagcg accgccgtcg
     2641 agcttgtcgg tgaccgcgaa gtggcctatc ggcgtctccg cgccgggccg gccgacgccc
     2701 acctcggccg aggccacctc gcgaccgtcc cgcacgagca cgagccgccg gtcggagcgg
     2761 tccaccagca cgcgcacgcg cgtgcgcgcg tgcgtgagag cgctgccgtc gtcgcggatc
     2821 cagccgaccc gcccgttcgg cagcgcggat gccagcacgc cgagccagcc ggcccggcgg
     2881 cgcacgacag gcaggaccat ggccgagccg aacggggtgc gggcgccgag caccgccagc
     2941 gtgcgcccgc cgggccgctc gcggagccgc agcgcacgcc cggaccggat ggcgaccctc
     3001 gacccggcgc ccccgcgcag ccgagcgccg gagtgccatg ggcgggcgcc ccgctcggtc
     3061 gcggcgccgt cggcctcgaa gctgcccgcg aacagcgggc ggtcgaacgt cggcgcctcg
     3121 cgggcatcgt cgcgggcgaa gctcgcgccg cccagcgtca gcgcgcccag cacgcccacg
     3181 accgccagta tccaggcttc cctgctcact tcgctcctgc tatcgggtac ggccccgccc
     3241 cgcgggggcc atacccccac ccggtcgagc gtacacgcct gatccgccgt ccccggccgg
     3301 tccgtaccga tggcatgagc gaacgcggaa cgaccgccga ggcggccctc gcaccggacc
     3361 tcgtggacgg gatctgcgag ctgacgctcg aggccgacga cctcgacctg ctcgcgggct
     3421 tctacaacga ggcgttcggc ttcaggacca tcaagcgcga cgacgaccgg gcctggctcg
     3481 cgtgcggatc gcgcacgcgg ctcggcatct ggagcccggg cccgaaggag ttcggcgacc
     3541 gcggcgggcg ccacgtgcac ttcgcgctgt ccgcggcacc cgaggccctc gacgcggtcg
     3601 ccgcccgcct gcgctcacgc tcggtgggct tccagggacc ggtcgagcac cccggcgggg
     3661 accggtcgat ctacgtccag gaccccgagg ggaacgtggt cgaggtctgg agcttcttcc
     3721 agcaggacgc cggggccatc gacggcgtcg acgccctcgc ctgacccgcg cgcgtccggc
     3781 aggcggcgca gcaccacggc ggcgctggcg agcgcgagcc cggcggccac gaccaggccg
     3841 atcccgagcg catccgtgaa ggcgcccccg gcggccgcgg tgagcgtgtc gcccaacctg
     3901 gccggcagct cggcagcgac cgcggtggcc gcgccgatcg actcgcccgc cgcgtcggcg
     3961 gccggcgcgg gcagccccgc cgccgcatcc tccacgcggc cggagtaggc cgagctggcc
     4021 accgagccga cgatcgcgac gcccagcgct ccggcgacca tccggttcac gtcgttcatc
     4081 gccgagccga cccccgcctt cgccagcggg accgcgctca tgacggcctc cgaccccggc
     4141 gccatgacgt tcgccatcgc gaacggcacg acgaagaaga acggaccgac gagccagtag
     4201 ctcgtgccgg cgtcccagaa caggacgctg gccagcgcgg ccgcgaggac cgtgagcgcc
     4261 cccgcgatct gccgcggcgc cccgatccgc ctgttgagct tgtggctcag cccggacccc
     4321 atcatcaggc cggcggccac cggcagcatc ttcacgcccg tctcgagggc cgagttgccc
     4381 tgcacgaact ggaggtactg ggtgacgagg aacagcatcc cgaacatcgc gaagaaggcg
     4441 gtcgagatcg cgagcgaacc gaccgtgaag gcccggcgcc ggaacagctc gaggtcgagc
     4501 agcggatccg ccgccgtgcg ctggcgcaag acgaacaggg cggcgagcac cacggccgcg
     4561 ccgagggcgg cgagcaccag gccgtcggtc cagccacggc cgggcgcctc gatgacggcc
     4621 cagacgaatg tgccgagggc cgcgatcgag agcgccacgc ccggtgcgtc gagccggccc
     4681 ggggccgggt cgcggctctc aggcacgtac ctcgcgccga gcgcgagcgc cacggcgatg
     4741 atcggcacgt tcacccagaa caccgccggc caggacgaga gctcgatcag cgtgccgccg
     4801 atcagcggcc cgaggccgat gccgatcgcg gccatcccgg cccagacggc gatcgccttg
     4861 ccgcgctcct cctgcgggaa cacgtccatg atgatcgaca gcgtcgtggg catgatcagc
     4921 gcggcgcctg cacccatcac ggcgcgcgcg gcgatgagct ggtcaccggt cgcggcgaac
     4981 gcaccggcga cgctggcgag gccgaacacc acgaggcccg cctggagcgc gcgcttgcgg
     5041 ccgtggcggt cgccgaggtt gccggccgcg agcagcaggc cggcgaagac gagcatgtag
     5101 gagtcgacga cccactggag cgtggagccg gagaggtcga ggtcgctctg caggctcggc
     5161 agcgcgacgt tcagcaccgt gttgtcgagc ccgatgacaa ccagcgacag cgacagcacc
     5221 gccagcgtcc tccacctgag ctcgtgcttg cggtcggcgg tcatggcttg tcccttcctc
     5281 ggtgttcggg gccggttgcc cggtgtccca atggttaaaa acgaatctcc gttctttata
     5341 ctgaaatgaa tgttcgctgt caagctagac tgcggcgacg aggtccgaag gacgaggagc
     5401 ggaatgccga aggtcactca ggagcacctg gacgcgcggc ggcgtcagat cctcgacgcc
     5461 gccctgacgt gcttcgcgcg cgagggtttc caccgtacga cgatgcagga catcttcggc
     5521 gaggcgggcc tctcccccgg cgccgtctac agctacttca agagcaagga cgagatcatc
     5581 gacgcgatca cgcgcgaggc ggcgcggttc gccgagacgg gcggcacgat gatccgcgag
     5641 aacgcggagg agctgacgct cgcgggggcc gtggccgctc tcgcgcacgg gttcgagcag
     5701 ctcgacgtgg ggacggcgga gacccgcgcg cgcatggtcc cgcagctatg ggtcgaggcg
     5761 cagcgcaact cgatcgtgcg cgaccgggcg cgcgtgggga tcgggggagc ggtggaggga
     5821 ctgaccgagg tcgcgcgcgc cgcgcaggag cgtggcgatc tcgatccgga gctcgatccg
     5881 gaggcggcgg cgcgggtggc gatctccgtg ttccagggcc tgctcgtcca gcgcagcatc
     5941 ttcggcgccg acgtggacgt cgatgcctac gtcgaggccg cgctgctgat gctcggcccc
     6001 cgcgcctagc cgggcacgcg ctcgccgaag agccgcgggt cgttgctgat cacggcatcg
     6061 acgccgagcg cctcgagccg gcggatcgcg ggctcctcgt ccacggtcca gacgtagagc
     6121 tccccgcccg ccgcgtgcac cgcgcgcacg agccgcgcgg tgacgaagcg ccagtgggcc
     6181 atcagggcgt cgcagcgtcc ggagcggatg aggcgcccgg cgcgcgcggg cacgagcagc
     6241 cgcgccacct ggagggcgcc gagcgcgggc agcacgagca gtggcgactg tgtgtagtcg
     6301 cgcctgacgt tcggcaccga aagcccgagc ttcaaccgcg gctcagccgc gcggatgcgg
     6361 tcgaggctct ccaggtggtg ggtcgagatc agcacccggt caaccaggcc gcgctcgcgc
     6421 agcgcctcga ccacgcgcag ctcgtagccg cgcgccttca ggtcgacgtt gaagtgcatc
     6481 gtcgcgaacg tctccccggc gaggtggtcg agcccctcct cgaacgtcag caccggcccc
     6541 tccgcgaggt gctcgtagtc gtggctcagc acgagctcgg tattcgcggg gtcgcgcagg
     6601 tcgcgcggca gcacgtcgaa ctcgatcatg tcgaccccgg cgtcgagcgc ggcgtcgaag
     6661 ctcgccacgg tgttccccgg cgcgatcagg ccggcgccct tgtgcccgac ccgcttcacc
     6721 tgtcgctagt cgcggtcgcg cgcgacgaac gcggccgccc agaggcacac catgtgcatc
     6781 gcctggtcca cgctcgcggt tagcttctgg tccacgtcgg ccggggtgcg cttcacgcgc
     6841 gtcatgtacg cgttgaccac gcggccgtcg tcgatcgcca tgtgcgggag cgcgatcagg
     6901 gcggcgacac cgaccgcgcg cgtcaggctc gtgcggcgtg ccacgccgag cagcgcgggc
     6961 acgaacgccg ccgtgtaggt catgccgtgg tggaggagcg cggtgcggga gtcctcgtcc
     7021 tcgccgagcc cgcccttctt gttgagcgcc tggaagtccg tctgtaggag gtagtcgccg
     7081 atgtggtgcg agacgaagaa atcggcgaaa ggagcgcggg cgggcatggg tcgaacgtac
     7141 tggatcgggt gcgtcagccg cagcggccga cgctgaggga accgcgcttg gccgtgaagg
     7201 tcgcgcgcag gacgccggtg gtgacgcggt agctccacgc gccgcgcggg aggcggcgcg
     7261 agccgatcct caccgagcac gggcggaacg ggcggcgcag cgtggcgagc gaggcctgga
     7321 ggcggtaggt gcgccgggcg cggccctcga ccttcaggcg ccagccgcgc gagccctcga
     7381 tcgacaccag ccgctcgcgc tcgtacatgc gcgactcgct gcgcccgcgg gggagggcga
     7441 tcaggtcgag tcgcccgctg cggtcgcgca gcctgacctg cccgctgccg gcgccgtagt
     7501 cggccagcgt gtcgacgtcg ggcgccagca gcgggagcac cgcgccggcg cgcaggagca
     7561 gcgggagctg gtcgagcggg gcgtcgacgg tgcgatcgcc ggggcgcaag cgggcggggc
     7621 ggccgagctt gaggcccccg gtgggctcgt ggtaggccag cgtgcgccag aggtcgaacc
     7681 agcggccgtt cggcacgtag agcctgcgct gcgtggcccc cggctcgagg acgggcgccg
     7741 cgagcaggtc ggggccgaac aggaactcat cgtcggtggc ggcggcgcgc gcgtcggccg
     7801 ggtaggtcag cgccaggtgg cgcatgagcg gcagcccggt gcggcggtac tcggcctcgt
     7861 gcgcggcgat gtacgggtag agctgcgtgc gcagcttcgc gtagcggcgc cagtggccga
     7921 cgatctcccg cgaccacggc tgcgggcgct cccccctggg cgggatggcg gtgccggcga
     7981 acttcgtccg cattacgccg gagacggctc cgaactggat ccagcggatc agcagctcgg
     8041 gagtgagctc gcgggtgccg agcgagaagt agccgccgat gtccgatccc caggtgctga
     8101 tgccggagag gcccatggtc agcgcgttgg tgaccgccga ggcgaggccg tcgtagcccc
     8161 agtcggtggt cgggtcgccg ccccagacga tctgcgcgta cgggtgcacg ccggtccagc
     8221 cggagcgcac gtggcgcgcg atcggccgcg cctggcggcg cgcgaactcc cagccggcgc
     8281 ggtggtaggg caccgggtag tagttgtgca tctgcgcgcc ggtcatcccg ttggcgtgct
     8341 gggagtcgag cggcgtgtac tcgccgaagt cctccatgaa gccgtcgtgg ccgtcctcga
     8401 cgagctggga cataaggccg ttccagaagt cctgcgcgcc gggtgccgtg aagtccacct
     8461 gcgagaccgg ggtctgcggc ggggtgcggc tccccacgta ggcgttgaac acgtacggct
     8521 ttccgctcga gtccttctgg aagacgccgc gcgcggcgcc gtcggaccac accggctcgt
     8581 actcctggca gatctccgag ttcacgtagg agagcgccgc gaggccgttg cggtggaaga
     8641 agtcggtgcg ctcgcgctcg gcggcgcggt gcgggacggc ctcgccacag ggcaggtagc
     8701 gcaggtgggt ctcgacggcg gaggcggggg cgtcggcctc gcgcagcgtc cggaccgcct
     8761 cctcctcgcg ggcgcgcggc ggcacgttct cctggccggt gtggaaccag gggccgagga
     8821 accacggcgc gagcggcttc ggctggcggc cggtgcgctc ggtgaggcgc ttcaccgtgg
     8881 cggcgggggt cggcccggca aggacgcgga acgcgatctg actcgcctgg gtctccatgc
     8941 tccacgcgtc cgaaagctcg ctgccgagcc ggtgcatgct cgcctcgttg ttgtcgatca
     9001 gcacgccgta gccgcgggtg gagagcagcc agggcatcgg gaagtaggtg gcgtcgtcgc
     9061 gcgggtggta gccccagggc gggacgaagg cggtgaggaa cgcgcgctcc tcgggttggt
     9121 acgggccctc cgcgacgtag ctctcgacct cgttgccgcg ctggtccacc gcattcgagc
     9181 gctcgccgaa gccgaggtag cgctccctgt cggcggcatc gaagccgacg ccgacggcct
     9241 ccacgtcgcc cgtgcccgcg gcgccggtca cggcggcggt cacgccgatc gcgccgtcgc
     9301 cggagggctc aatccgcacg gcgagccggc ggccgagcgg gtcggtggtg gcgagctcgg
     9361 ccgtgagggc ccgtccgtcg ccggcgctgg tgagggcgcg ggtggcgtgc caccagccgg
     9421 tcgaggtgcg aaagccgatc gtccccgcgg gaccggttcc ggtcgcatgc gcctcggcga
     9481 gcgtgggacc gcccggctgg acgaactcga cgtgccacgg atcgaggtcg atacgcgcct
     9541 cgatcgcccc cgcggggacg gtctccgcgg cggcggctgg agcggcgagg gccaccgcga
     9601 gggccagggc cgccacgagg gtccgggctg gggagcggtc cacccgggca ccgtaaccgc
     9661 ccccgcagga cctccgcgcc gccgcttttc cttcggcttt cctgacttat gggcgctatg
     9721 gacccttaag tcaggaatgt cgatcagttc tgcggccgag gcggtaggtc aggtgctcga
     9781 gccggcccgc cgtggtgcgc aggctcgcgt ccagcctggc cgcgtggccg ggcttcacga
     9841 gtgccttggg gctcgtggcg ttgcgcgtgt agagggtgag cttcacgctc accgcgccgg
     9901 cggggaggcc gctcacgcgg agggtccccc gcccgagggc gagacgggga gcgctcgcgc
     9961 cgcgggtggc gaggccgccc gcgcgggtga ccctgacggt ccacgtgcgc gacttcgcgc
    10021 ccttccggta gaccgtgatg cgaccggcca cgcggccggc atcgccgcgc tgcggcagca
    10081 tggcgcgcgg ggccgtgaac gcgagcgtct tgatcggctc gtcgctgtcg acgcgcaggc
    10141 gcaggccggt gccggtgctc gacttccact gggacgcgcc gagggccgcg gagggagcgg
    10201 tgccgcagtc gcccgacgtg gtgccggagc cgccagtggt ggagccgccg ggggtggtgg
    10261 agccgcttgt ggtggagccg ccggtggtgg agccgcagtc cggggtggtg ccgccgggcg
    10321 tggtgcccgg ctcggtgccg ggggtcgagc cgccgggggt cgagccgccg ggggtcgagc
    10381 cggcgggcgt ggtgccgggc tcggtggtgc cgggcgtgga gccgcctgga gtcgtgccgc
    10441 cggtggagcc gcctggagtc gtgccgccgg agtccgcggg gggcggcggg ggtggtacgg
    10501 gcttcgccgt cacgaccgcc gtggggtcgg tcgccgcgct gcggctcccg ccggcgttcg
    10561 cggcggtgac gacgccgcgc actcgcgcgc ccacgtccgc aaggacgagc gtgtagctgg
    10621 ccgcggttgc accggcgatc gggacgcagc ccgagccgtc gacgccgcaa cgctcccagg
    10681 cgtacgtgaa ggtgagcggg gcgctgccgg tccagctccc gtcgctcagc gccagctcct
    10741 cgccctcacg cgccgtgccg gtcagtgtgg gggccgtcgt gttcgccggc gggatcgcct
    10801 gcaccacggc ggtcggcgcc gactcggcgc tgcgctcgcc accgccgttg gcggcggtca
    10861 cgagcacccg gatcgtcgcg ccgacgtcgc tcgcggtcag cgtgtacgtg gttccggtgg
    10921 cccccacgat gttcgcgcag tcgttgccgc ctgcgtcgca acggcgccag cgccgggtgt
    10981 acgtgatcgt cggcgtgccg gtccaggagc cgtcgtccgc ggtgagggtc agtccgtcgc
    11041 gcgcggtccc cgagatcttg ggaagggtgc tgttcagcgg agggcccacc ccgacggctc
    11101 cggtgggatc ggaggtcaag gccgcggagc cgccggcgtt ggtcgctgtc accaccacgc
    11161 ggatggcgtg ccccacgtcg acaggggtca gctcgtatga ggcgcccgta gcgccgctga
    11221 tgttcgcgca gccgttgccg gccgagtcgc agcggcgcca ctggtaggcg aaggagatcg
    11281 tcggggtgcc ggtccacgag ccggtcgtgg tcgtgagcgt ctggctgtcg cgcgcgttcc
    11341 cggagaccac cggcggagcc gtgttgaccg gcgggctcgc cgtgacggcg ccggtggggt
    11401 cggaggtcga ggccgcggag ccgccggcgt tggtcgcggt caccaccacg cggatcgcgt
    11461 gccccacgtc ggccggggtc agctcgtacg cggcgcctgt cgcgccggag atgctcgcgc
    11521 agccgttgcc ggccgagtcg cagcggcgcc actggtaggc gaacgtgatc gtcggcgtcc
    11581 cggtccacga cccggtcgtc gccgtaaggg actggccgtc gcgcgcggtt ccggacacga
    11641 cgggcggagc gttgttggcg ggcgggcgtg cggtgacggt gcccgtcggg tcggaggtcg
    11701 aggcggcgga gccgccggcg ttgctcgcgg tcacctcggc gcgcagcgtc ttccccacgt
    11761 ccgcagggac gagcgtgtag ctcgtcccgg tagcccccgc gatgtcgacg cacgcgtcac
    11821 cgcccgaatc acaccgctgc cactgatacc cgaacgaaat cgtcggagtg cccgtccacg
    11881 acccgttagt ggtcgtaaga gactggccgt cgcgcgcggt gccggagacc acgggcgccg
    11941 cgttgttgac cggcgcgcgt gcggcgacgg caccggtcgc gtcggacgtc gaggccgccg
    12001 agccgccggc gttgctcgcg gtgaccaccg cgcgcagggt cttgcccacg tcggccggca
    12061 cgagcgcgta gctcgatccg gtggcgcccg cgatgtccac acacgtgttc cccgccgaat
    12121 cgcagcgccg ccactggtag gtgaaggaga tggtcggcgt accggtccac gacccgttcg
    12181 tggtcgagag cgtctgcccg tcacgcgcgg tcccggagat cacgggcggc gcgttgttgg
    12241 tgggcggccg cgccgcgacc accgcgctgg gcgcgctcac ggccgaagcg ctgccgccgc
    12301 cgttcgtgcc ggtgacctcg gcgcgcagcg tcttccccac atccgccggg acgagcgcgt
    12361 aggtcgaccc cgtagcgccg gcgatgtcca cgcacgagcc gccggcggag tcgcagcggc
    12421 gccactggta cccgaacgaa atcgtcggag tcccagacca cgacccgttc gtcgtagtaa
    12481 gagactggcc gtcgcgcgcg gttccggaca ccaccggcgg agcgttgttg accggcgcgc
    12541 gtgccgcgac ggtgcccgtc gggtcggagg tcgaggtggc ggagccgccg gcgttgctcg
    12601 cggtcacgac gacccggatc gtcttgccca cgtccgccgg gacgagcgcg tagctcgatc
    12661 cggtggcgcc cgcgatatcg gtgcacacgt cgccggcgga gtcacaccgc cgccactgat
    12721 acgcgaatgc gatcgtcgga gtgcccgtcc acgacccggt cgtcgtagta agagactggc
    12781 cgtcgcgcgc agttccggac accaccggcg gagcgttgtt gaccggcgcg cgcgccgcga
    12841 cggcaccggt cgcgtcggat gtcgcggccg cggagccgcc ggcgttgctc gcggtcacga
    12901 ccacccggat cgtcttcccc acatccgccg gcaccagcgt gtagctcgac cccgtagcgc
    12961 cggcgatgtc cacgcacgag ccgccggcgg agtcacaccg ccgccactgg tacccgaacg
    13021 aaatcgtcgg ggtccccgtc cacgacccgt tcgtggtcga gagcgcctgc ccgtcgcgcg
    13081 cggtcccgga taccaccggc ggagcgctgt tggcgggcgg gcgtgcggtg acggtgcccg
    13141 tcgggtcgga ggtcgaggcg gcggagccgc cggcgttgct cgcggtcacc tcggcgcgca
    13201 gggtcttccc cacgtccgcc ggcaccagcg tgtaactcga cccggtggca cccgcgatgt
    13261 cgacgcacgc gtccccaccc gaatcacacc gctgccactg atacgcgaac gaaatcgtcg
    13321 gagtgcccgt ccacgacccg gtcgtcgtag taagagactg gccgtcgcgc gcggtcccgg
    13381 acaccaccgg cggagcgccg ttggcgggcg ggcgtgcggt gacggcgccg gttgcgtcgg
    13441 aggtcgaggc cgcggtgccg ccggcgttgc tcgcggtcac gaccacccgg atcgtcttcc
    13501 ccacgtccgc cggcaccagc gtgtagctcg accccgtagc gcccgcgatg tccacgcacg
    13561 cgttccccgc cgaatcgcac cgccgccact ggtaggcgaa ggagatggtc ggcgtaccgg
    13621 tccacgaccc gttcgtggtc gagagcgcct gcccgtcgcg cgcggtcccg gacaccaccg
    13681 gcggagcgtt gttgaccggt gcgcgcgcca cgacgacccc agtagcccca gagctcgcca
    13741 cgcccgacga caccacgttc cgcgccgtga cctgcacgcg gatcgtcttg ccgacgtccc
    13801 ccgcacccac cacgtacgtc gaccccgtgg ccccgtcgat gtccacgcac gccgcgccgg
    13861 ccgagtcgca gcggcgccac tggtacgaga acgtcatcgg cgtcgtgccc gaccaggtgc
    13921 cgttgccggc cgtgagcgtc tggccgtcgc gagcggtccc gctcaccgtc ggcgccgcgg
    13981 tgttcgcggg ggcgctcgcg gcctggtccg agaccagcgt catcgcgccg ggcataacga
    14041 ggtcggtcgc gacctccatg tgcaggatcg cgctcgtggc gtcgttgacg aggaagccgt
    14101 cgatccccat gatgttcgcg tccatcccga gcgtgttgat gtagctcggg ttcctcgtgg
    14161 tcacgtgcga cccgtattcg gtgatcgacg cgttccagta gttgttcagc gcgtgcagcg
    14221 gatccacgag gtcgcggccg ttgatccgcg ccgtgtccgg ctgcacgccg aggtcgccct
    14281 cgtaggcgag catcccgaag tcggcatcga cggtcccgct gggcggcgtg cggaacccgg
    14341 tgatcgggac gtcggccgcg tcgtcgccgc cgaagtcgcg gtagccgtcg tagaggccga
    14401 gccagcggac gggcttcgac gggtcgcggt aggccacgac cagaccccag ccgccgtact
    14461 ggtccttgcc ggtggaggcc tggacgttcg ccgcggtgta ggtcccggcc ccggcctggc
    14521 ggacgagcga ggtgacgtcg gcgaacccct ggtagcggct cgggaagaag cggtcgctgt
    14581 cgaccgtcga ggccgtgatc gtccggtagc cggctccgtc gggggcggcg aggcgcatcg
    14641 tgccgcgcgc cgccgggttc ggcgcgtcca cgccctccgg catcgccttc gtgtccgagc
    14701 cccagtagag cccggcgtaa aggatcgtgg cgccggccgg cagcctcagg tcggcgctgc
    14761 tcgagtcgaa cgtcgtcgag tcggagtcga tgtcgatcgg caccatcacc caggcggagt
    14821 tgcgcagcac ggtgccggtc ccgttgcgcg cgttcgcgca gttcggcgcg ccctcggggc
    14881 aggtcacgat cgtgttggcg gccgtggtga tgtcgccgcg ctcgacctgg ccgtagcgca
    14941 gcgagtaggc ccgatcggca ctggcggagg cggccgggag cacggcgaga ccgaccacca
    15001 gagcggcgag cagcgtccgg atgcggttgg accagatcaa gggggtacgc gctgcgccgc
    15061 gatccgtcac ggcgcccctg ccaggggtcc cccgattatg acgcagaaaa gccgattacg
    15121 ccaggacgaa atcggccgga tcgggccgcc tcgtgcggcg cttgtactcg gtcacgaacc
    15181 cggcccagtt gttggtgacc ctcccggatt cggtccggta ccagctgttg cagcccgcgg
    15241 cccaaacgct gtcgccgagc cgctcctgca cctcgcggtt gaaggccgac tggacgtccc
    15301 cgcggacgtc catccagcga gcccccgagc tgccgaggtc gcgcaccgcc tcgaccacga
    15361 atccgatctg gctctcgagc atcgtgatga tcgatccggc ccccaggttc gtgttcggcc
    15421 cgtagagcat gaacatgttc gggaagccgc tgatcgccag gccgagatag gcctcggcgc
    15481 cgtcgcgcca gacgtcgttg aggtcggcgc cgtcgcgccc gcggatcgcc atcggcgcca
    15541 ggaactcgtt cgcctggaag ccggtgccga gcacgatcgc gtcgacctcg cggacccgcc
    15601 cgtcggcggt cgcgacgccg gtcgcggtga cctcatggat gtcgtcggtc acgacctcga
    15661 cgttcgggcg cgcgagggcc ggcaggtagt cgttcgagat cagcacgcgc ttgcagccca
    15721 tcgggtagcc cggcacgagc ttctcgcgca gctgcgggtc ggggatctcg gtgcgcaggc
    15781 ggcgcttgta gccgagctcg tagagcctca gcatccagta ggcgcgggtc atggcgagcg
    15841 tgaacagctc gaagcggatg aacgtgagcc ggcggctgat cgcggggatc agcggaatgc
    15901 gccggaagag cgccttctcg cgcgggcggt aggggcggtc gggcttgggg atgacccagc
    15961 cgggcgtgcg ctggaacacg tgcagtcgct cgaccacctt cgcgacctcc ggcacgtact
    16021 ggacagcgct cgcgcccgtc ccgatcgccg ccacgcgctt gccggccatg tcgtagtcgt
    16081 ggttccagcg ggccgagtgg aacagctcgc cgcggaagtc gtcgagtccc gggatccgcg
    16141 ggaacgaggg gcgcgagagc tggccggtcg cggtcacgag cacgtcgaac tcggcctcct
    16201 cgccgccgac caggcgcagg acccagcggc cgcgctcggc gtcgaagtcc gccgcggcca
    16261 cctcggtccc caggcgcatg tgctcgcgca gcccgtgctt gtcggtgagg cgctcgaggt
    16321 aggcgaggat ctcgtcgcgc tgcgggaagc ggcgcgtcca gtcgtggttg ggctcgaagc
    16381 tgaactggta gaggtgcgac ggcacgtcgc acgtgaggcc cgggtacgag ttgtcacgcc
    16441 agaccccgcc caccgcgccg gccttctcga agatcgtgaa cgactcgatc ccggcctgct
    16501 ggagcttgat cccgaggccg atcccgccga agccggcgcc gacgatcgcg acgctcggcc
    16561 cctgcctacg cgacccgttc cccataccgc cccgacacgc tagcgcgcgc ccgggctacc
    16621 agctgctgtc ggagtcgcgc gtgaagacgc gcttcttcga ctggttcggg tactggcggt
    16681 tccacaccgc gatcgcgccg cccaccaccg cgctgatcac gacgccgaac acgacccagt
    16741 cctcccagcc gtcgaccgcg tccagcacca ggtagccgag gcccacggcc accgccagca
    16801 cgatcaggat ctggaccacg gcggtacctc tcacttaagc tccccctcgc tcggtcggac
    16861 ggacagtacg gaccgtcgga ctcaaaggca agccggggga ggctgtgatg gtcaagacga
    16921 tcgccgtggg caccgacggg tccgacaccg cgaaggtcgc ggtcgacttc gcgctcgaca
    16981 tggctgagcg ctacggcgcg aaggtcgtcg tcgcaagcgc gtacaagccg gtgtcggaga
    17041 gccagctccg cagggagcag gccgaggcac cggaggacat ccagtggtcg atcaacccga
    17101 gcgaggaggt cgacacgacc ctccgcgcgg tcgaggagga ggccaaggaa cgcggcctga
    17161 gctacgcgag cgaggcgcgc aacggcgatc ccgccgaggt gctgtgcgac atcgccgagg
    17221 cgcacggcgc ggacgtgatc gtggtcggca accggggcat gcagcggcgc ctgctggggt
    17281 cggtgccgaa ctcggtcgcg cacaaggcgc cgtgctcggt gatggtggtg aagacgacct
    17341 agcgcgcgag cgcgggcggg ccgcctccac ctccccaccg cgcgcgcagc gcgcgccacg
    17401 ccttccggat ccgcttgacg aggaaggtcg cgagcgcgat gccgctgacc agcaggatca
    17461 ccgcgacgat cgcggcgggc acggggtgct cgatcgcgag caccgtgacg ccggccacga
    17521 gcccgtcctc gagcaggctc accgcgatgt tcgacgccgg ctccggcgag ctgttcacgc
    17581 cgagccggat gccggccttc accgcgtggc tcgcgaacgc catcgcaccg gcgccgcctc
    17641 cggcgaacac ctcctcgacc cccgtcgccc ccgcgtcccc gccgagcgcc acgccgaccg
    17701 cgctcgcgat ggcgggtcgc acgatcgtgt ggagcacgtc ccacgcgctg tcgagcagcg
    17761 gcaccttgtc cacgacgaac tcgatcgcat agagcacgag tgcggccgcc agcacgccgt
    17821 tgccctgcag ctcctccggc acgtcgcccc agcccgcccg gccgagcagc gacatcagcg
    17881 caacggtgag gtaggcgttc acgcccgacg cccagcctga gctgatcagc gtcgagacga
    17941 ggtccatcta cgccgctcgg cgctcgcgtt ccaggcagcg gtagagcgcg tcgcgcagcg
    18001 ccttctcgcg cgggtcgttc gcgatgtggt agcccatccg gttcatcacg taggcgaacg
    18061 acacctcgtt ctccgggtcg gcgaacgcga acgagccgcc cgccccgggg tggccgaacg
    18121 cgcgcgggct ggcgaagtcg agctcgccgg gccgcgggcg ggcgaagccg agcgagaagg
    18181 cggtgtcgat cttcagcacc tcgtcgtgcc agccgcgggc gggcgggcgc ggcgtggcgg
    18241 tcagctctgc gagggtcgcg tcggagaggc cgagctcggg cgggcgcgcc gcgaacgcca
    18301 tgtacgcccg tgcgacgctg cgcgcggtgc cgagcgcgcc gcccgccggg aactcgaggt
    18361 ggcggtagcg gcggctgtcg aacgacgccg gcgagtacac gcgcgggttg gcgaacgcct
    18421 tgaaggtgag cgagcgcggg ttggccatgg cgagcgccat ccgcggcggc atgtccctga
    18481 ggcccggcag cgacctgatc ggagcgcgct cgatccgcgc gatgcgccgc ttcggcacgt
    18541 cctccggcac gccgaagtgc acgtcgaggc cgagcggcgc ggcgatctcc tcggcgaaga
    18601 agcgccccag cgtgcggtgg tcggggtcga tccgccgcac gagctcgccc tcgtaccagc
    18661 cgaggctgag gccgtggtag ccgtggcggg tgccgggctc ccagaccggg cgctggcgag
    18721 cgatcacctc ggctaggcga tcgaagtccg cgatgagcgt cggggtgagc ggctcgtcga
    18781 ccaccggcag accggcctcg tggccgagga gctggcggac cgtaacgcgc tccttgcccg
    18841 cctggccgaa ctcgggccag taggtcgaga cgcgctcgtc gtagtcgagc agcccgcgcg
    18901 tgtggagcag cgcgagggtc atcgcggcga ggcccttcga ggtcgagtag acgatcacga
    18961 gcgtgtcctc gtcccagcgc gcgccgctcc gcgcgtcgcg cacgccgccc cacaggtcca
    19021 ccaccttctc gccgcccacg taggccgcgc acgcggcgcc gagctcgcga ccctcgctga
    19081 ggttgcgctc gaacgcgtag cggacggcct cgtagcccgg caccaccgac ccgccgacgc
    19141 cggggctcac gcggcctccc gccggccgac caggagcgac tgcgccgcgc gcccgatcag
    19201 gccgtgctca tcgtgcagca cgctctcggc catgcccacc ccgtccgggt ccacgacggt
    19261 gcggctgtcc agcccgaccc ggtcgccgcg cggggggcgc aggaggtgga tcgtgaggtc
    19321 cgcgttgatg aagacgtgcg tgttccagtc gagcgaggcg ctcacgccgt tgccgaagtc
    19381 ggcggcggcg gccgcacgct ggaccgccga cggctcctcg ccatcgacca ggggcacgag
    19441 gaagcgcacc caggcggcgg ccgggcccgg ctccgtgagc gagccgcgca cgaaccgcag
    19501 ctccaccccg tcgccggcga acatcggccg ctcgccgggt ggcgaggacg agggggcgac
    19561 gccgtccggc ggcggcagcg ccggctcgtg cgccggcacc gccggtgcgg tcccctccac
    19621 ccgcgcgagc acgaggccga gcacgcggca caccggcccc gccgtggtct cgagcgtcga
    19681 ctcgatcagc tgcgcgcggc ggccggggcg gacgacccgc gtcgcgatcg acaggggctc
    19741 gagcggaacc ggccgcagga actcgtaggt gagccgcgcc accaccagct cgccggcctc
    19801 gagctcgacg gaacgagcca gcagggccgc gggcgcgccg ccgtgctgcg cgcgcggatc
    19861 ccacggtccg cgggccagct cggttggcac gaaggcgtcg ccctcgcgtt cgtagatcgc
    19921 caccgcgact aggcccgccg caccagcgtc accccgtcgc ggaccggtag ctgcaccacg
    19981 acgagccgct cgtcggccgc cagccggtcg ttgagcgcgg ccagcgcggt gccggactcg
    20041 tcgtccgggt cggggtcgag gacctggccg ctccagagcg tgttgtcgat cacgatcagt
    20101 ccgcgcgggg cgagcttcgg gagcacggcg tcgacatagg ccgagtagcc ggtcttgtcg
    20161 gcgtcgacga acacgaggtc gaacggcccg tcgagctcgg cgatggtctc gagcgccggc
    20221 ccgagcctga cctcgatcct gtccgcgtag cccgccgcct cgatgtggcg ccgtgcgaac
    20281 tcggcgtgct tctcggagac ctcgcagctc acgatgcggc cgccgtcggg cagcgcggcg
    20341 gccatcgaca gggccgaata gccggagtag gtgccgatct cgagcaccgc ccgcgcgccg
    20401 gaggcccaga ccagcatctc gaggaagcgc ccctccacgc ggccggtgag catctgcggg
    20461 aactcgaggt gctcgcgcgt ctccgcggcc aggcgctcga gccagcccgg cggcgcggtg
    20521 ctgtgctcct cgcagtagcg gtcgatctcc ggtgggttga cctctggcac ggcgtggcag
    20581 cctacgccac cggtaccgtg ccgctccgtg cggagggccc tcctcgtcgc ggtcgtgctg
    20641 gcgctcgtcg ccggcgacgg cgtcgccgcg gcggccaccg ccaagctgcc gcgcgggggc
    20701 accacgctct tcccgcgcca gcgcgtggtg gccttcgccg gcacgccggg ggcgcccgcg
    20761 ctcggcgcac tcggtgtcgg ccggctcgac cacgccgtgc ggcggctgcg ccgccaggcg
    20821 cgccactacc accgcgccgg gcgccccatc ctgccggccc tcgacctgct cgccacgatc
    20881 tgctcggcgg ggccgggccg cgacggcaaa taccggtacc gccgcccgct gaagatcgtg
    20941 cagcgccacc tggccgccgc ccgccgggcg cgcgctctgc tgctcatcca catccagccc
    21001 ggacgctcga cgttcatgga ggagctgcgt ccctaccgcc gcttcctgtc cgagcccgac
    21061 gtcggcgtcg cgctcgaccc cgaatggcgg atgggccccg gccagcgtcc cggcaaggtc
    21121 atcggctcgg ctgactccga cgagatcaac gcggtctccg cctacctcga ccgggtcgtg
    21181 aagcgtcacg atctgcccca gaagctgctg gtcgcccacc agttcacgcc gaacatgatc
    21241 cgccgccgcg cgcgcgttcg ccagcgcccg ggcgtggcgg tgacgatctc gatcgacggc
    21301 gtcggatcgc ggcggctgaa ggagggcacc tactcgcggc tcgcgcgctc gggcgacaac
    21361 ctctacgacg gcttcaagct cttctacgag gaggacgggc gcgtcatgac gcccgcccag
    21421 gtgctgagac ttcggccgca gcccgacttc gtcgtgtacg agtagcaagc agccaggccg
    21481 ccaccacgcc gccgatcccg ccgaacaggt ggccctgcca cgagatcccc gcgcgcggca
    21541 ccagcccgct cagcaggacg ctgccccaca gcgcgcccac gatcagtccc atgaagagct
    21601 cgaacgcgtt gcggttgtag aacccgcgca cgagcaggta ggccgcgtaa ccgaagacga
    21661 ccccgctcgc cccgatgtgg ttcgtgtgct ccggggcgac gagccaggtg ccgaccccgc
    21721 ccaccgccat cacgatcgcg gtcacgctca gcacccggac gaggccggcg agcgcaatcg
    21781 cgaaccccat gacgaggaac ggcacggtgt tcgacatgag gtgcgagaag tcggcgtgca
    21841 ggaacgggga ggcgacgatg ccgaccagcc cgtccggctc gcgcggctcg atcccgtagt
    21901 cgtcgaggcc ggtgcccacg gtggcgtcgg cgatctcgag cacccacatg accgcggcca
    21961 tcccgagcac gagcaggaag cccgcctggc gatcgctgcg tggcttgtct acggcctcgg
    22021 cgatgcgggc agtatcgcgg ctggcggtgt cgaacccggt caggtaggct cgcgccgagc
    22081 aaccgagggg ggtcccacat gggagagcag gagtccggtc cgcgcgtgaa cccggaggat
    22141 gtcgcgaagc ggcagccgcc gctcgcgaag gtcatggagg cgctggcgga gcccgaggga
    22201 aagacccgcc gcgactcccc tacggcgacg gcgcgcgaca tcgcgctcct catggaggca
    22261 cgcgacgcga gctccccgct cgcgtcggcg ctcgacgagc tcgaacgcgc gcacgggggc
    22321 aaggcgtccg aggacgacct caagccgcac ctggacccgt cggactaggg ccgcggcgct
    22381 cgacccactc agacggatga caccgagagc ggccgtccgc gccgcgctcg tgaacccgcc
    22441 gcacgtgcgc ggcccggggc ggccactcga gaacgcatcc cctcccctgg gcatcgcgta
    22501 catcgccgcg gcgctgcggg ccggcggctt tccggccgag ctctacgacc tggcagacga
    22561 ggtcccgatg gacccggcga agctcgccgc cgccggattc ttcgaccacg acctgtacgg
    22621 gttcacctcg tacacgaaga cgttcgacgc cgcgctcgag ctggtccgcc tgctgaaggc
    22681 gaacaacccg gccgcgacca tcgtcttcgg cgggccgcac gcgacgcccc tggcggaggt
    22741 gctcgtcgag acctacgacg agatcgacgt cgtggtccgc aacgagggcg agcaggcgat
    22801 ggtcgcgatc gcgcgtcacc gcgcgggcga tctcgactcc ctcgccggcg tggcgggcct
    22861 cgtgtaccgc aaaggcgggg cgatcgctcg caccggcgac ccgcacgacc tgccgccggt
    22921 cgattccctc cccgatcccg cacgcgatta cgcgatcagg ccgcagcgcc tggtctacga
    22981 ccggcgcgac ccgctgaatc cgcagcgcat cgagttcttc tcgagcagca ggggctgccc
    23041 gaagcgatgc acgttctgtt cgatcgtggt gatgagcccg cggtatcgct tccgctcggt
    23101 cgaatcgctc atggacgaga tccgcgcggt ccacgagcgc gatccgttcg ggcacgtcgc
    23161 gttcctcgac gcgaacttct tcgtccactg gcggcgcacc gccgagttcg cggaggccct
    23221 ccaccgctgg gatcctggcg tgacgtggtc ggcgaccgcg accgtcgacg cgatcgtgcg
    23281 gcacccgcgg gtggtcgagc ggatcggagc cctgaactgc cagatgctcg aggtcggcgt
    23341 cgagaacggc agcgacagct tcctcgcgcg cgtgaacaag cggacgaccg tggagcagaa
    23401 cctcgatgcg atccgcgccc tcgacgacgc gggcatcgag ctcgagctcg actacatcat
    23461 gttcgacccc gagaccacgc tcgaggagct ggccgacaac gcggacttcc tcaccgaggc
    23521 ggagctgacg gactacatcc cggccgacca cctcgccaac acgctcaggc tgtaccccgg
    23581 cacgcccgct cgctcgcgtt acgcccccgg tggacccggg ccagcggagt tcgccgcggc
    23641 cgacctgtac gcgccgatcg ccgatccgcg cgcggcggcg gtgaaccgcg tgatgaccga
    23701 ggcggtcgac acgtgcttcg tgggcctcga gcaggtcgtc cgcgcgtgcg agcgcgcggc
    23761 ggacacgagc cacgacccgc aggagatgca cgcgctcgct atcgcgttcc gcaacgtccc
    23821 gttcgcgatc ttccgcgaga gcatcgaccg cgccggcgcc ggcgagctgg acctcaccct
    23881 ctcggacctc gcggccagcg cgcggctgcg cgagctcgtg gcgctcgcac acgagcagga
    23941 gacacgcgct tcgcgcccgg ccgagctggc caatgccgtc tagctcgccc gctcccggag
    24001 tcggcgggga ggccgccgcc tccgcgttcc gcctgcggcc gaatccggag gtgcgagtgg
    24061 tcgaggagca cgggacggtg tacctgatcc cgcccgtggg acgcccgctg cggctcaatc
    24121 gcagcgcgtc ggggctctgg cggatgctcg cggagggacg gccacgcgac gaggtcgtcg
    24181 accgccacgc cgtcgacagc ggctgcgacg tcgcgaccgc ccgcgacgag atcgagcagc
    24241 tcatcgcatc cctgtgcgag aaccactacc tgatgcgcgc ccccgccgat gcgccctcgt
    24301 gaccgcatcc tgacgacggg gacgcgcttc gagcgcgtcc cgccgaaccc ggctcagccg
    24361 cgcctgcgtg tcgcgctgat caacccgccg gacccgacct cgtccgacga cggtcgctcg
    24421 ttcccagcgc tgggactcgg atacgtcgcg gcgttcgcgc gcgtgcgcgg cttccacgtc
    24481 gacctgttcg acttcgcgct cgacctcgat ccggacctcg acttcctcga ccgggtgggc
    24541 gcgctcgacg gctacgacgt ctacggcgtc tcgacctact ccgacaccct gccgtcggcc
    24601 ctgacggtcg cggcggcgct caggcggggc gcaccccgct ccccgatcgt cttcggcggc
    24661 taccacgccg cggtgctgga cgccgagctg ctcgaggact tcgacatgat cgactacgtc
    24721 gtgcgcgacg agggcgagat cccgttcagc gagctgctcg acggcctggc gagcgaccgc
    24781 tccgaccacg ggagtgtggc cagcctgacg tggcgaggcg cggatggcgc ggtcgtgcgc
    24841 aacccgtcgt ccgagcgttt gctcgaccag gccgagcttc cgttcccggt cacggagacg
    24901 cgccacggcc cgcgccgcta tgccgactac gccgaccgcg atagcggcga gcggcgccgc
    24961 gcggtgagcc tcgtcagcag ccgcgggtgc ccgaagcggt gcacgttctg ctcgatcgcg
    25021 ctcgtcaacc ccctgtggcg cgcgcgctcg gccgaatcga tcatggcgga ggtgcgctac
    25081 cggtacgcgc acgagccgtt cgcccacgtc ttcttccagg acggcaactt cttcgccagc
    25141 ccgaagcgct cgctcgcctt cgcgcgggag ctccacgcct tcgacccgtc gatcacgtgg
    25201 tcgggcacgg ccacccccga caacgtggtg cgccacgccg gcgtcctcgc cgagctggcc
    25261 gcgctcaact gcgcctcgct cgagctgggc atcgagagcg gcaacgaggg ctcgctcgaa
    25321 cgcttcggca agggcaccga ccgcgccgtc aacctccagg cgattcgcct gctcgagtcc
    25381 gccgggatcc ggctcgggct cgacttcatc atgttcgagc cggagatgac gctcgacgac
    25441 gtgagggcca acctcgactt cctccacgag gccgacctct acggctactg gcccgccgag
    25501 tgcctgttcc aggagctgcg gctctacccg gggaccaaga tgcgcacgcg tcacgtgccc
    25561 ggcgactgca cgcgtcacga ggtgccgccg actccactgc gcgacgccgg gctgcggacc
    25621 gcgttcgagg aggcctgggc gtaccgccgg accctccagg cggcaaccga cgaggcgctc
    25681 aagcagctcg aggtggcgat cgccggcctc gatgccgtcg accgtgacgg ggaggagccg
    25741 gagatccggc ggctgcgcca ggacgcgcag gcgtgcgtga tcgagctgcg gcacgccccc
    25801 tacgcgttcc tcgaggccgt cgcgagcccg tctcaggcag ctaggccgga cgagctggag
    25861 gccgcccgca ccgccacccg ctgggcgctc gccggcgcgg tcgcgacggt gcgttccgtg
    25921 cgcgcggcga tcgggacctg gacggcaccg gcgcgcacgc gagccgagcg cgtggcatga
    25981 gcgtctcggc cgagttcctc gcggcccggt ggcagaccgc ccccgaggcg ctccccggcc
    26041 tcgcggcgga ctggctcgac cgcgactggg tcgcgcggtt cgagtcgtgg gccgtgccgc
    26101 ccacgacggc gcgcctgttc gtcctccagg aggacggccc cgcgggcgcc gcgacggcgg
    26161 ccacgatgcc cgacccgcgg gtggcatacg agctgttcca gcgctacgcc ggcgagggac
    26221 agcagctcac gctgctgctg aaccacaccg agcgcgtggc gcccgagcta cgtgagcttc
    26281 gtgacgggct cggaatcggc aacccgtggc gccacgacga cgtggtggcg acgctgtcca
    26341 ccgccggcag cgggatcggc taccacgcgg gacacgagga cagcctcgtg gtgcagctcg
    26401 ccgggcggcg gcactggcgc gcctggtcgg ccgaccacgt gccggcctcg caccggcacg
    26461 agctggtctg gcgccacgcg ccggaggggc cgggcgcgat caggcgcccc gccgcggatc
    26521 cgctcgtcga ctgcgagctg ggccccggcg acgtgctgta cgtgccctcg ctcttcccgc
    26581 acgagggccg cacgctcgag ctgtcggtga gcctgtcgat cgcgtggcga ggggtgtcgg
    26641 tggcgacgct gctggacgag gtcgaggccc tgcccgccgc ggagcggacg cttacggagc
    26701 gccaccccga cgccttcctc cggctggtgc gcgacccgcc gccgggtgcg ccggacgcgt
    26761 gcgccgccgt gatggagcag ctcgatccgg cgctgcgcgc actccgtgtc tccgcgaccg
    26821 aacgcgagtg ggtgcgccgg tccgtggacg agctgctggc cggctgagcc cccgaggggg
    26881 cccagcggcc gtggagcggt tcgctacagc ggcatcttcc cgcgcgcgcg gcgtccgagc
    26941 gaagcgctct tgttggtcgc gcggcgcgag ctgccgaacg gcttctggag ccagcgcccc
    27001 aggaagcccg gcgcggatcc ggcggcgctc tgaccgactc cgagcgtcct gtcgacgccg
    27061 cgctgcggat ggcgcgagag cctcggcagc aggaaggcga gcaccagcag tacgacacat
    27121 acggccacga tcgcggcgat ggtcatgtct ctctcctcag gtagcggggg tacgcgtgcc
    27181 cc
//
LOCUS       DASVPL010000203        15064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_63313, whole genome shotgun sequence.
ACCESSION   DASVPL010000203 DASVPL010000000
VERSION     DASVPL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15064
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63313"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VF517_10710"
     CDS             complement(<1..559)
                     /locus_tag="VF517_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX8053453.1"
                     /translation="MELDRHFIERNDFSAARRGYDPDEVDRHLRELADAVADLKRQQK
                     PSPASLAGAAADQVRTIVEAAERSAAEIQQNAEDEARRITDDASRKARETRERADADA
                     AQRVADADEATQRALDRAGSIEGEIDRLLGDLRSSANGLVERVNGAAGSLQGELDQAR
                     SEFESVREARLEVGGGGGGGGAALRD"
     gene            complement(642..2651)
                     /gene="uvrB"
                     /locus_tag="VF517_10715"
     CDS             complement(642..2651)
                     /gene="uvrB"
                     /locus_tag="VF517_10715"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX8053454.1"
                     /translation="MPKFEINPAYTAVADQPQARDGLAAGIEAGERFQTLLGVTGSGK
                     TATMAFTIEQVQRPALVIAHNKTLAAQLCNEFREFLPKNAVEYFVSYYDYYQPEAYVP
                     SADLYIEKDSSINEEIDRLRHAATSALFARRDVVIVASVSCIYGLGSPEKYDAQMLLL
                     KKGEMADRDQILRKLVEIQYSRNDQALSRGSFRVRGETLEVFPAYAETAFRAVFFGDE
                     IEAIHHFDPLTGELYNELEHVGIWPATHYATDRPTIERAVKEIRDELEARCAELEREG
                     KLLESHRLRQRTQFDIEMMKELGFCNGIENYSRILDGRPPGSRPYCLLDYFPEDFVCF
                     VDESHQTIPQIGGMFEGDRSRKQTLVDYGFRLPSAMDNRPQTFDEFLARTPQIVMVSA
                     TPGDFERGHSSRIVEQIVRPTGIIDPHVEVRETKNQIDDLMNEVRARAEAGERALVTT
                     LTKKMAEDLTDYLMEYGFRVRYLHSEIDTLERIQIIRDLRLGEFDVLVGVNLLREGLD
                     LPEVTLVAIIDADKEGFLRGETSLIQTIGRAARNVKGRVIMYADKESAAMRKAIGETD
                     RRRAIQVAYNEEHGITPESIKKGISDVGDFLSLESKVPQRSHKARARAAREQGMAPSE
                     IQKTIVELEEEMLLAADDLRFEYAAKLRDEIKALRAELDEQQATA"
     gene            2712..2978
                     /locus_tag="VF517_10720"
     CDS             2712..2978
                     /locus_tag="VF517_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053455.1"
                     /translation="MQTSGVLLCSIAMLVGAAVGAPAAGAQELDTGQVRDAGGQPHDF
                     DRNDNFIVCFNHENGAVIDDRPDPETGFASCPRPFGLVPVFLPL"
     gene            3014..3631
                     /locus_tag="VF517_10725"
     CDS             3014..3631
                     /locus_tag="VF517_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053456.1"
                     /translation="MRPALALLLAAVVSVALLAAPAGATHSRGKCTQRGKTLIRNDSG
                     RLFEKDNVGGGLTLLGCLWSANRTVVLDTAYSDFTTDHTYGQERLAGRYAAWATSTED
                     VSCKAECPADYPTVRFGVHVMNLRTRAERTVEGIPAGSAFKLNADGVTAWLARLGNGQ
                     REVHLWTAKSHDVVGSGAVPSSSLRLTRDTLSWVDGDVQHTVRLP"
     gene            3678..4667
                     /locus_tag="VF517_10730"
     CDS             3678..4667
                     /locus_tag="VF517_10730"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053457.1"
                     /translation="MPPDETERSDTQGWEREFALRRPRYQRLAEEVKFALETELSTAG
                     IKTHDVIARCKTLESLREKIDRKRYDQPLEQAEDLAGVRVVTLFLSDLPRVEELVRRH
                     LDVLSEEDTIEADEPAVFGYMSKHYVGTLAARYAGPRYDDLEGLKVEIQLRTILMDAW
                     ASVSHHLAYKSDSSIPRELRRQFSALSGLFYVADLQFEALFAGSTLVQHQASAALEAG
                     GSTAEVDLNLDTLAAWLRDRFPDRAHSDREQVARLVEQLKQVGYTTLGEIESLVQQAA
                     PVFDHRERGRDQAYSDVGAVRVSVALVNPQFDALRLKKVLDDMKQRDEPRSGG"
     gene            complement(4664..5263)
                     /locus_tag="VF517_10735"
     CDS             complement(4664..5263)
                     /locus_tag="VF517_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8053458.1"
                     /translation="MSRAVRRRRRTAVALIAAGLIAGIGLALWFGPGEDAIREITLPL
                     RHEDIIRQQSREKGLDPALVAAVIYQESKFRDQTSHAGARGLMQITPATAEFIARDSG
                     GTAFSQEDLAKPQINIAYGTYYLRYLLRRYDGDTELALAAYNAGETNVDRWAREAGGE
                     DEFNRDDHIPFPETREYVDNVFELRDEYRRHYKRELGLR"
     gene            complement(5260..5871)
                     /gene="coaE"
                     /locus_tag="VF517_10740"
     CDS             complement(5260..5871)
                     /gene="coaE"
                     /locus_tag="VF517_10740"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX8053459.1"
                     /translation="MAGSAARERIPFVGLTGGIGAGKSEALAALERAGAATLSSDAVV
                     HELLASDPVRDELTERFGPDIAPGGTVDRDRMAEVVFGDPAQRAWLEGYLWPKVGERI
                     WSWREELDGRSERPRAAIVEVPLLFESGMDQAFDHTIAVVADDALRDERIGARGHSGV
                     ASRESRQLPQDEKAERADFAVRNDGTVQELEEVLIGVLERIER"
     gene            complement(6041..7651)
                     /gene="rpsA"
                     /locus_tag="VF517_10745"
     CDS             complement(6041..7651)
                     /gene="rpsA"
                     /locus_tag="VF517_10745"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX8053460.1"
                     /translation="MPEGDTLIAEPPKAVEGSNGLLLEINGEIVPNYDATIVPFSEGD
                     VVSGNVVRIDKDEVLVDIGYKSEGVIPSNELSIRKSANPHDEVELGEEVDALVLTKED
                     QDGRLVLSKKRARFEKAWRKIEGAAESGEPVEGSVIEVVKGGLIIDLGVRGFLPASLV
                     DIRRVQNLDEFLGQMIECKVIELNRSRNNVVLSRRAVLEEERKEVRQQILDRLQPGQI
                     VEGAISNIVDFGAFVDLDGIDGLIHISELSWSHVNHPSEILSIGQVVPVKVLDIDRDR
                     QRISLGLKQTQEDPWQRVVDTYALGDELEGKVTKVVSFGAFVEILDGVEGLVHISELA
                     QHHVENPREVVNQNDVIKVKILEIDSERRRLSLSAKRVEGQELPLGGGDYLPPSGGTG
                     EEGEIGEVHDLGLSDVVFAGAEGEAPAPVEDAPVEPAPVGEGDVDAPPVEPAPPVEPA
                     PPADDEPANEAPAEDIAAPDAAGDAPAPVEDAPVEEAPPATDAVSAEGDAPLPDAPAP
                     GDAISGAPADAVEAEGDAAAPPDDAPAT"
     gene            7689..8816
                     /locus_tag="VF517_10750"
     CDS             7689..8816
                     /locus_tag="VF517_10750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8053461.1"
                     /translation="MAKRVTKRGAERTPLGGDYDVLICGASFAGLSVARELAGSGART
                     LLIDRYAVGERQTSACAAPTDWLTALGLEESIRQTFRDLVFHTPHGDARYRLPFTFST
                     FDYRQLCRLLFEQCDAEFETAKVDGRDGDVVHTDRGDLRAPLIVDALGWRRVLASEGY
                     QPPDAPLSRGLEVHPPGSGGDLELWIDRRYVPAGYAWSFPARDEVRVGVGSFDPRFHV
                     KDTTVLLAEDLDVPAVRYQGNWIPHRLRDSTEDGIFFAGDSAGHCLPLTAEGIRTAFH
                     FGIACGRELRAVVDGRRTREDALRRYHDFSAEHEWKFRWMLRAQQAVPRVPPRLLGPL
                     IRALGAQRVVDWAFGHYLRIAPPPPPTVARRPAPAREPVAA"
     gene            complement(8813..9583)
                     /locus_tag="VF517_10755"
     CDS             complement(8813..9583)
                     /locus_tag="VF517_10755"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEX8053462.1"
                     /translation="MAGWLPPQPPPGRGHRRRPARARAPRLLNERLPHAGLYRRATAA
                     AFVLAPVLFLVDNLLHPKEYARDNEAKQLAEIADHYTRWQIAHLLALLAILGFAVAVL
                     GLAFLVRRHRPVAGLVGGVLALLGVLSLASVVALDGFTWGILGEVSARTGDTRVTAQA
                     LHDVQQSEWSLQYYVPALAFTVGMVYLAVTAARSGALPEPAGWLLALGSLLVGTEGLI
                     VSNAYYLIGSVVMLAAGVAVALGLVRMGDDDFARPRGR"
     gene            complement(9499..9801)
                     /locus_tag="VF517_10760"
     CDS             complement(9499..9801)
                     /locus_tag="VF517_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053463.1"
                     /translation="MEPPLVEERRRLPDRRRTERAESRGRYPRKAAAAAVGVCGGLAL
                     VYLFFAAIGAVDLAEALGASAVALVMGLVWLGGYLLSRRQAEGTVGDRLERERRGF"
     gene            9896..10492
                     /locus_tag="VF517_10765"
     CDS             9896..10492
                     /locus_tag="VF517_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8053464.1"
                     /translation="MAMVVVALGVAVEAGTSVVSFDRGANRAVHAHAAEPVTGSAFVL
                     SALGSTFVLVGVVLAASLGLAVRGRRRASVALISAYVVTDATVALMKVLVARPRPGES
                     LTHAGGFSFPSGHAATSMAVYGSLAFVFARARRGLPRVAFALAGGALVTLIGLSRIYL
                     GVHYPSDVVAGWITGAAITIAAWTIASRLRPRRAPAPA"
     gene            complement(10489..11388)
                     /locus_tag="VF517_10770"
     CDS             complement(10489..11388)
                     /locus_tag="VF517_10770"
                     /inference="COORDINATES: protein motif:HMM:NF025600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HEX8053465.1"
                     /translation="MRGIICIGGALAALAVGLAACGEGDATAKGKDLDLTRLAVADDN
                     VRTSGAKRGYLYLCRDQMGGGGAQQDGPWIDGDRWDYTRKAMVDGDVEWREAVFSQAV
                     SGSTRTLRGNGLPTNHTTGTYPVSSSDDAYQYDRNPNSIRAGSVDLQLPKNPKKLSSA
                     QCIQGGQVGILDSGVAMFSAVDATGRDAGAHEVQDRCDGHPQIQGTYHYHALPRCIAT
                     GSSKAHSKRIGWALDGFPIYGPRGTGGEYMRNSELDACHGHTHTVKIDGRKRSTYHYH
                     ATMEFPYTVGCFRGKQMATDRMP"
     gene            complement(11471..11911)
                     /locus_tag="VF517_10775"
     CDS             complement(11471..11911)
                     /locus_tag="VF517_10775"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8053466.1"
                     /translation="MKPGVAVLLTACAGGLVAMQAPINSALGKAAGTFAAASISFAVG
                     TTALIGITLLSNSKVDLAAIGDLRWYYLTGGLLGAAYVTTVLVSVRTLGAGGLTAATI
                     TGQLSFSVVMDRLGAFGLDEKPLSVGRVIGVALLAAGVFLIVRE"
     gene            complement(11908..14634)
                     /gene="polA"
                     /locus_tag="VF517_10780"
     CDS             complement(11908..14634)
                     /gene="polA"
                     /locus_tag="VF517_10780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX8053467.1"
                     /translation="MTADETREIFLIDGNSLVYRAFFALPETIATSKGQPTNAIFGFA
                     SMLVKILTEHGPKSTVVVWDAGMSGRDEVSADYKAQRKSRPDLLREQWPHFEPLVEAF
                     GYRNVKVPGYEADDVIATLAERARAESIDVTIVTGDRDAFQLIEPREDGRGEVKVMAT
                     SRGITETKVYDRQAVIDRYGIAPELIPDFYGLKGDTSDNIPGVPGIGDKTASELLQRF
                     GSLETVLASVDEISGAKRKENLTNHADDARMSKQLATAVRDVELDIDLESCLADEPDR
                     GRLREVFREFELRAPLERLEEALGEDAAAPREIAATVLSARAVEGPTAALRELDGTLA
                     TLVAERPVPEDDPTVLSQGDTVPPGQESPDEAAPLELSLDVEAASPQPPLRFAAYAGG
                     ETVLTGEAESLAAIAAAWGERPVVAHDWKSLAASEEPSQAPPLQHDTIVAAYLLDPAG
                     RAYPLHELAEREGLGAKIDPPSEGQGTGNGLAERAVVTRAVAEIQRAELEKEGLTRLF
                     EEVELPLVDVLVDLERAGVKLDTAKLAEISAKVQEQVETLEGEIHELAGTEFTIGSPQ
                     QLGEILFEKLQLSRKRRGKTGFSTDARVLQAIRGEHEIIPKIERWRELSKLKSTYLDA
                     LPLLVDQRSRLHTTFNQTSTTTGRLSSTDPNLQNIPIRTELGREIRACFVAEEGHRLI
                     SADYSQVELRVLAHVCGEEVLKDIFARGEDVHTATAAEMFGVEPKDIDPGTRSKAKMI
                     NFGIVYGLSAYGLADRLDIPQDEAGEFIARYLDRFPKVRQFIDSTIADATESGYVTTI
                     FGRKRRIPELRSRKWQTRSLGERLAVNTPIQGSAADIIKVAMVRCENALRDAGLSTRL
                     VLQIHDELLFEAPVAEVDAASEIVVREMADAYELDPPLAVDAGSGENWLAAK"
     gene            complement(14644..>15064)
                     /locus_tag="VF517_10785"
     CDS             complement(14644..>15064)
                     /locus_tag="VF517_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053468.1"
                     /translation="NGRRVRALSGDLFGPVSGERFDLITANPPYVPGDEIPTAGAARA
                     WEGGFDGRALVDRFAAGVAEHLSPGGRVLMVVSSLTGEEETLAALRAGGLRAEVVARE
                     RGPLGPLVSARAELLERRGLLAPGEREEDMLVIEARA"
BASE COUNT         2066 a         5303 c         5324 g         2371 t
ORIGIN      
        1 cgtcgcggag cgcggccccg ccgcctccgc ctccgccgcc gacctcgagc cgcgcctcgc
       61 ggaccgactc gaactcgctc cgcgcctggt cgagctcgcc ctgaagcgag ccggccgcgc
      121 cgttgacgcg ctccaccagc ccgttggccg aggagcgcag gtcgcccagc aggcgatcga
      181 tctccccctc gatcgagccg gcgcgatcga gcgcgcgctg ggtcgcctcg tcggcgtccg
      241 cgacgcgctg cgcggcatcg gcatcggcgc gctcgcgcgt ctcccgggcc ttccgcgagg
      301 cgtcgtcggt gatccggcgc gcctcgtcct cggcgttctg ctggatctcg gcggcgctgc
      361 gctcggccgc ctccacgatc gtgcggacct ggtccgcggc cgcgcccgcg agcgacgcgg
      421 gcgagggctt ctgctggcgc ttgaggtcgg cgaccgcgtc cgcgagctcg cgcaggtgcc
      481 ggtctacctc gtcggggtca tagccccggc gcgcggccga gaagtcgttg cgctcgatga
      541 agtgacggtc cagttccaag ttctctcccg tgggaatggc ggggccttac ggaggcgtgc
      601 ggcgcggagc gtaccgcaac ggggaccgcc tttcgacgcg atcaggccgt ggcctgctgc
      661 tcgtccagct cggctcgcag ggcctttatc tcgtcgcgca gcttggccgc gtactcgaag
      721 cgcaggtcgt ccgcggcgag gagcatctcc tcctcgagct cgacgatcgt cttctggatc
      781 tccgagggcg ccatcccctg ctcgcgcgcg gcgcgggcgc gcgccttgtg gctgcgctgg
      841 ggcaccttcg actcgagcga gaggaagtcc ccgacgtcgg agatgccctt cttgatcgac
      901 tccggcgtga tgccgtgctc ctcgttgtag gcgacctgga tcgcgcggcg gcggtcggtc
      961 tcgccgatcg ccttgcgcat cgcggcggac tccttgtccg cgtacatgat cacgcgcccc
     1021 ttcacgttgc gggcggcgcg accgatcgtc tggataagcg acgtctcgcc gcggaggaag
     1081 ccctccttgt cggcatcgat gatcgcgacc agggtgacct cgggcaggtc gaggccctcg
     1141 cgcagcaggt tcacgccgac cagcacgtcg aactcgccga gccgcaggtc gcggatgatc
     1201 tggatccgct cgagcgtgtc gatctccgag tgcaggtagc ggacccggaa gccgtactcc
     1261 atcaggtagt cggtgaggtc ctcggccatc ttcttcgtca gcgtggtcac gagcgcgcgc
     1321 tcgccggcct cggcccgggc gcggacctcg ttcatcaggt cgtcgatctg gttcttggtc
     1381 tcacggacct cgacgtgcgg gtcgatgatc cccgtcggcc ggacgatctg ctcgacgatc
     1441 cgcgacgagt gcccgcgctc gaagtcgccg ggcgtggccg acaccatcac gatctgcggc
     1501 gtgcgcgcga ggaactcgtc gaaggtctgc gggcggttgt ccatcgcgct cggcaggcgg
     1561 aagccgtagt cgaccagggt ctgcttgcgc gagcggtcgc cctcgaacat gccgccgatc
     1621 tgcgggatgg tctggtgcga ctcgtcaacg aagcagacga agtcctccgg gaagtagtcg
     1681 agcaggcagt acgggcgcga gccgggcggg cggccgtcca ggatccgcga gtagttctcg
     1741 atcccgttgc agaagccgag ctccttcatc atctcgatgt cgaactgcgt gcgctggcgg
     1801 agacgatggg actcgagcag cttgccctcg cgctcgagct cggcgcagcg ggcctcgagc
     1861 tcgtcgcgga tctccttcac ggcgcgctcg atcgtggggc ggtcggtggc gtagtgggtg
     1921 gccggccaga tgccgacgtg ctcgagctcg ttgtagagct ccccggtcag cgggtcgaag
     1981 tggtggatcg cctcgatctc gtcaccgaag aagaccgcgc ggaaggccgt ctcggcgtag
     2041 gccgggaaga cctcgagggt ctcgcccctc acgcggaacg agccgcgtga gagcgcctgg
     2101 tcgttgcggc tgtactggat ctcgaccagc ttgcggagga tttggtcgcg gtcggccatc
     2161 tcgcccttct tcagcagcag catctgcgcg tcgtacttct cgggcgagcc gaggccgtag
     2221 atgcacgaga cgctggccac gatcaccacg tcgcggcgcg cgaacagcgc gctcgtggcg
     2281 gcgtggcgga ggcggtcgat ctcctcgttg atcgaggagt ccttctcgat gtagaggtcc
     2341 gcgctcggga cgtacgcctc gggctggtag tagtcgtagt acgagacgaa gtactcgacc
     2401 gcgttcttgg gcaggaactc acggaactcg ttgcagagct gcgccgccag cgtcttgttg
     2461 tgcgcgatca cgagggccgg ccgctggacc tgctcgatcg tgaaggccat cgtggcggtc
     2521 ttgcccgacc cggtcacccc gagcagcgtc tggaagcgct ccccggcctc gatcccggcg
     2581 gcgagcccgt cgcgcgcctg cggctggtcc gcgaccgccg tgtaggcggg gttgatctcg
     2641 aacttcggca tcctcgcctg aagctagcag cggcatttga ccggacggga gttccgtgtt
     2701 ttgctgaaga tatgcaaacc tcaggtgtcc tgctgtgctc gatcgcgatg ctggtgggag
     2761 ccgccgtcgg ggcgccggcc gccggggcgc aggagctgga caccggccag gtccgcgacg
     2821 caggcgggca gccgcacgac ttcgaccgca acgacaactt catcgtgtgc ttcaaccacg
     2881 agaacggcgc cgtgatcgac gaccggccag acccggagac cggtttcgcc agctgtccta
     2941 ggcccttcgg gctcgtcccg gtatttctac ccctgtaggc catcgccggc gccgcggcgg
     3001 ttaggctccg ggcatgcgcc ccgcgctcgc tttgctcctg gccgcggtcg tctccgtggc
     3061 gctgctcgcc gcccccgccg gggccacgca ctcacgcggc aagtgcacgc agcgcggcaa
     3121 gacgctgatc aggaacgact ccgggcggct gttcgagaag gacaacgtcg gcggcgggct
     3181 cacgctgctc ggatgcctgt ggtcggccaa caggaccgtc gtgctcgaca ccgcatacag
     3241 cgacttcacg accgaccaca cctacgggca ggagcggctc gccggccgct acgcagcgtg
     3301 ggccacgagc accgaggacg tgagctgcaa ggccgagtgc ccggccgact acccgaccgt
     3361 ccggttcggc gtgcacgtga tgaacctgcg cacgcgcgcg gagcggaccg tcgaggggat
     3421 cccggcgggc agcgcgttca agctcaacgc cgacggcgtg accgcctggc tggcgcggct
     3481 tggcaacggc cagcgcgagg tgcacctgtg gaccgcgaag agccacgacg tggtcggcag
     3541 cggcgcggtc cccagctcgt cgctgcgcct gacgcgcgac accctgagct gggtcgacgg
     3601 cgacgtgcag cacaccgtcc ggctgcccta gctagcggag gccgagctcc ctctccgcgc
     3661 ggacgtatct ttcgcggatg ccgcccgacg aaactgaacg ctcagacact caaggctggg
     3721 agcgagagtt cgcacttcgc cgtccgcgat atcagcgctt ggcggaagag gtgaaattcg
     3781 cacttgagac tgagctatcg accgctggca tcaagacgca cgacgtcatc gcacgatgca
     3841 agacgctcga aagcctccgc gagaagattg atcgcaagcg atacgaccag cctctcgaac
     3901 aggccgagga tctggccgga gttcgggtag taacgctctt cctgtccgac ctcccgaggg
     3961 ttgaggaact cgtgaggcgt cacttggacg tgctctcaga agaggacacg atcgaggcgg
     4021 atgagccagc cgtcttcggt tacatgtcta agcactacgt gggcacgctc gccgcgcgct
     4081 acgcgggacc ccgttacgac gatctggagg gcctaaaggt cgagatccag ctccgtacga
     4141 ttttgatgga cgcgtgggca agcgtctctc accatctcgc gtataagagc gattcgagta
     4201 tcccgcgcga gcttcggcgg cagttctcag cgttgagcgg actgttctat gtcgcggacc
     4261 tacagttcga agccctgttt gccggctcca cgctggtgca acaccaagcg agcgcagctc
     4321 tcgaggccgg gggctccaca gcagaggtag acctaaatct ggacaccctt gcggcgtggc
     4381 tgcgcgatcg atttccggat cgcgcgcact cagatcgaga acaggtcgca cgccttgtcg
     4441 agcaactcaa gcaagtcggc tacacaacgc tcggcgagat cgagagcctt gttcagcagg
     4501 cagctccagt cttcgaccac cgtgagcggg gacgagacca agcgtactca gatgtcggcg
     4561 cagtgcgagt ctcagtcgcc ctagtcaacc ctcagttcga cgcgcttcga ctaaagaagg
     4621 tcttggacga tatgaagcaa cgcgacgagc cgaggtccgg cggctagcgg aggccgagct
     4681 ccctcttgta gtggcggcgg tattcgtcgc gcagctcgaa cacgttgtcc acgtactcgc
     4741 gcgtctcggg gaacgggatg tggtcgtcgc ggttgaactc gtcctcgccg ccggcctcgc
     4801 gcgcccacct gtccacgttg gtctcccccg cgttgtacgc ggccagcgcc agctccgtgt
     4861 cgccgtcgta gcggcgcagg aggtagcgca ggtagtacgt cccgtaggcg atgttgatct
     4921 gcggcttggc gaggtcctcc tggctgaacg cggtgccgcc cgagtcgcgg gcgatgaact
     4981 ctgcggtcgc gggcgtaatc tgcatgagcc cacgcgcgcc ggcgtgcgag gtctggtcgc
     5041 ggaacttcga ctcctggtag atgaccgcgg cgaccagcgc cgggtcgagg cccttctcac
     5101 gcgactgctg gcggatgatg tcctcgtggc gcaacggaag cgtgatctcg cggatcgcgt
     5161 cctcccccgg gccgaaccag agcgccaggc cgattccggc gatgaggccg gccgcgatca
     5221 gggcgacggc cgtgcggcgc ctgcggcgca cggctcggct catcgctcga tccgttccag
     5281 tactccgatc agcacctcct ccaactcctg cactgtccca tcgttgcgca cggcgaagtc
     5341 ggcacgctcg gccttctcgt cctgcgggag ctggcgcgac tcgcgcgagg ccactccgct
     5401 gtgcccccgg gcgccgatcc gctcgtcgcg cagcgcgtcg tcggcgacga ccgcgatcgt
     5461 gtggtcgaag gcctggtcca tgccggactc gaacagcagc gggacctcga cgatcgcggc
     5521 gcgcgggcgc tcggagcggc cgtcgagctc ctcgcgccag ctccagatgc gctcgccgac
     5581 cttcggccac aggtagccct cgagccaggc gcgctgggcg gggtcgccga agacgacctc
     5641 ggccatccgg tcgcgatcga ccgtcccgcc gggcgcgatg tcgggaccga agcgctccgt
     5701 gagctcgtcg cgcacgggat cgctcgcgag cagctcgtgc acgaccgcgt cgctcgacag
     5761 cgtggcggcg ccggcgcgct cgagcgcggc gagggcctcg gacttccccg cgccgatacc
     5821 gccggtcagc ccgacgaacg ggatgcgctc gcgggccgcg gagcccgcca cgcgctaggt
     5881 ggtcggtgcc gggttcgcgt gcccgcgggg gcgcgaaaac cgagctggcc gaggacgcag
     5941 ggtgatgtcc atgcatgagt gcatggacga acccgaggac gctggcccag cgaaggtttg
     6001 cagcgtcatc cgggggtgcg ggagcccggg accggccacc tcaggtcgcg ggggcgtcgt
     6061 cgggcggagc ggcggcgtcg ccctcggcct cgacggcgtc ggcgggcgcg ccggagatgg
     6121 cgtcgcccgg agccggcgcg tcgggaagcg gggcgtcgcc ctcggcggag acggcgtcgg
     6181 tggccggcgg agcctcttcg acgggtgcgt cctcgacggg agccggagcg tcgccggcgg
     6241 cgtcgggcgc cgcgatgtcc tcggccggag cctcgttggc cggctcgtcg tcggccggcg
     6301 gggcgggctc caccggcggg gcgggctcca ccggcggggc gtccacgtcg ccctcgccga
     6361 ccggcgctgg ctccacgggg gcgtcctcga ccggggcggg cgcctcgccc tccgcgccgg
     6421 cgaagacgac gtcggagaga ccgaggtcgt ggacctcgcc gatctcgccc tcctcgccgg
     6481 tgccgccgga gggcggcagg tagtcgccgc ccccgagcgg cagctcctgg ccctcgacgc
     6541 gcttggcgct gaggctgagc cggcggcgct cggagtcgat ctcgaggatc ttcaccttga
     6601 tcacgtcgtt ctggttgacc acctcgcgcg ggttctcgac gtggtgctgg gcgagctcgg
     6661 agatgtggac gaggccctcg accccgtcga ggatctccac gaacgcgccg aagctcacga
     6721 ccttggtgac cttgccctcg agctcgtcgc cgagcgcgta ggtgtcgacg acccgctgcc
     6781 acgggtcctc ctgcgtctgc ttcaggccca gcgagatccg ctggcggtcg cggtcgatgt
     6841 cgagcacctt caccggcacg acctgcccga tcgagaggat ctcggagggg tggttcacgt
     6901 ggctccagga gagctcggag atgtggatca ggccgtcgat gccgtcgagg tcgacgaatg
     6961 cgccgaagtc gacgatgttc gagatcgccc cctcgacgat ctggcctggc tggagtcggt
     7021 cgagaatctg ctgccgcacc tccttgcgct cctcctcgag gaccgcgcgg cgcgaaagta
     7081 ccacgttgtt gcgggaccgg ttcagctcga tgaccttgca ctcgatcatc tggccgagga
     7141 actcgtccag gttctggacg cggcgaatgt cgaccagcga ggcgggcagg aagccgcgca
     7201 ccccgaggtc gatgatcagg ccgcccttga cgacctcgat gaccgagccc tcgaccggct
     7261 cgccggactc cgcggcgccc tcgatcttgc gccatgcctt ctcgaagcgg gcgcgcttct
     7321 tggagagcac gagccggccg tcctgatcct ccttggtgag gaccagcgcg tcgacctcct
     7381 cgccgagctc gacctcgtcg tgagggttcg ccgacttgcg gatcgacagc tcgttcgagg
     7441 ggatgacgcc ctcgctcttg tagccgatgt cgacgaggac ctcgtccttg tcgatccgga
     7501 ccacgttgcc gctgacgaca tcgccctcgc tgaaggggac gatcgtcgcg tcgtagttgg
     7561 gaacgatctc gccgttgatc tcgaggagga gtccgttcga tccctccacg gccttcggcg
     7621 gctcggctat aagcgtgtct ccctctggca ccgggcgcaa gagtagcggc aagctagcct
     7681 ctccaccgat ggcgaagcgg gtcacgaagc ggggcgccga gcggacgcct ctcggcggcg
     7741 actacgacgt cctgatctgc ggggcgagct tcgcggggct gtccgtggcc cgcgagctgg
     7801 cgggcagtgg cgcgcgcacg ctgctgatcg atcgctacgc ggtgggcgag cgccagacct
     7861 cggcctgcgc ggcgccaacc gactggctca cggcgctggg gctcgaggaa tcgatccgcc
     7921 agacgttccg cgacctcgtc ttccacaccc cccacggcga cgcgcgctac cggctgccgt
     7981 tcaccttctc cacgttcgac taccgccagc tctgccggct cctgttcgag cagtgcgacg
     8041 ccgaattcga gaccgccaag gtcgacggcc gcgacggaga cgtggttcac accgaccgcg
     8101 gcgacctgcg cgcgccgctg atcgtggacg ccctcggctg gcggcgcgtg ctcgcgagcg
     8161 aggggtacca gccgccggat gccccgctgt cgcgcgggct cgaggtgcac ccgcccggct
     8221 cgggcggcga tttggagctc tggatcgacc gccgctacgt gcccgccggc tacgcctgga
     8281 gcttccccgc gcgcgacgag gtgcgggtcg gcgtgggcag cttcgacccg cgcttccacg
     8341 tgaaggacac caccgtgctg ctggcggagg acctcgacgt gccggccgtg cgctaccagg
     8401 gcaactggat cccgcaccgg ctgcgcgact ccaccgagga cggcatcttc ttcgcggggg
     8461 actcggcggg tcactgcctg ccgctcacgg ccgagggcat ccgcacggcg ttccacttcg
     8521 ggatcgcgtg cgggcgcgag ctgcgcgcgg tggtcgacgg ccgccgcacg cgcgaggacg
     8581 ccctgcgccg ctaccacgac ttcagcgccg agcacgagtg gaagttccgc tggatgctgc
     8641 gcgcgcagca ggcggtgccg agggtgccgc cgcggctgct cgggccgctg atccgcgcgc
     8701 tcggcgcgca gcgcgtggtc gactgggcgt tcgggcacta cctgcggatc gcacccccgc
     8761 cgccgcccac ggtcgcccgg cggccagcgc cggcgcgcga gcccgtcgcc gcctagcggc
     8821 cccgcggccg cgcgaagtcg tcgtcgccca tgcggaccag gccgagcgcc acggccaccc
     8881 ccgccgcgag catcaccacg gagccgatca ggtagtacgc gttcgagacg atcagcccct
     8941 cggtgccgac cagcagcgac ccgagcgcga gcagccagcc ggccggctcg ggcagggctc
     9001 cggagcgggc tgccgtgacg gcgaggtaca ccatgcccac cgtgaacgcc agcgccggca
     9061 cgtagtactg gagcgaccac tccgactgct ggacgtcgtg cagcgcctgt gcggtcacgc
     9121 gggtgtctcc ggtgcgcgcg gagacctcgc cgagaatgcc ccacgtgaac ccgtcgagcg
     9181 cgacgacgga cgcgagggag agcaccccga gcagggcgag cacaccgccg accagacccg
     9241 cgaccgggcg gtggcggcgc accaggaaag cgagcccgag cacggccacc gcgaaaccga
     9301 ggatcgcgag gagggccagc aggtgcgcga tctgccagcg cgtgtagtgg tcggcgatct
     9361 cggcgagctg cttcgcctcg ttgtcgcgtg cgtactcctt cgggtgcagg aggttgtcga
     9421 cgaggaacag caccggcgcc agtacgaacg ccgcggccgt ggcgcggcgg tagaggccgg
     9481 cgtgcgggag ccgctcgttc agaagccgcg gcgctcgcgc tcgagccggt cgccgacggt
     9541 gccctcggcc tggcggcggc tgaggaggta gccacccagc cagacgaggc ccatgaccag
     9601 cgcgaccgcg ctggccccga gggcctccgc gaggtcgacc gcgccgatcg ccgcgaagaa
     9661 caggtacacg agggcgaggc cgccgcagac gcccaccgcg gccgccgctg ccttgcgcgg
     9721 gtaacggccg cgactctcgg cgcgctcggt ccggcggcgg tcgggaagcc tgcgccgctc
     9781 ttcgaccagg ggcggctcca tgcccatttt tatctccctc ccacgtgcgc ctgggatggt
     9841 aatcggagtt ttgcgtgagc tcaggcgcat ccccgcagtc gcctggcctg ccgcgatggc
     9901 catggtcgtg gtcgcgctcg gcgtggccgt cgaggccggg acgagcgtcg tcagcttcga
     9961 ccgcggcgcc aaccgggccg ttcacgccca cgccgccgag ccggtcaccg ggtcggcctt
    10021 cgtgctcagc gcgctcggct ccaccttcgt gctcgtcggg gtggtgctcg ccgcgtcgct
    10081 tggactcgct gttcgcggcc gccggcgcgc ctccgtggcg ctgatctccg cgtacgtcgt
    10141 caccgacgcc accgtggcgc tcatgaaggt gctcgtcgcc cgcccgcgcc cgggcgagtc
    10201 gctcacccat gccggcgggt tcagcttccc gagcgggcac gcggccacga gcatggcggt
    10261 gtacgggagc ctcgccttcg tattcgcgcg tgcgcgccgg gggctcccgc gcgtggcctt
    10321 tgcgctcgcg ggcggcgcgc tcgtcacgct gatcggcctc agccggatct acctcggggt
    10381 gcactacccg agcgacgtcg tggcgggctg gatcaccggc gcggcgatca cgatcgccgc
    10441 ctggacgatc gcgagcaggc tgcgcccgcg gcgggcgccc gcgccggcct agggcatgcg
    10501 gtcggtggcc atctgcttcc cgcggaagca gcccacggtg tacgggaact ccatcgtcgc
    10561 gtggtagtgg taggtcgagc gcttcctgcc gtcgatcttc acggtgtgcg tatggccgtg
    10621 acaggcgtcg agctcggagt tgcgcatgta ctcgccgccc gtgccgcgcg ggccgtagat
    10681 cgggaagccg tcgagcgccc agccgatgcg cttcgagtgc gccttgctcg acccggtcgc
    10741 gatgcagcgc ggcagcgcgt ggtagtggta ggtgccctgg atctgagggt ggccgtcgca
    10801 gcggtcctgg acctcgtggg cgccggcgtc gcggccggtc gcgtcgaccg cgctgaacat
    10861 cgccacgccc gagtcgagga tcccgacctg cccgccctgg atgcactgcg ccgacgacag
    10921 cttcttcgga ttcttcggca gctggaggtc cacgctcccc gcgcggatcg aattcgggtt
    10981 gcggtcgtac tggtaggcgt cgtccgacga cgagacgggg taggtgccgg tcgtgtggtt
    11041 ggtcggcagt ccgttgccgc gcagcgtccg cgtcgacccg ctcaccgcct ggctgaacac
    11101 ggcctccctc cactcgacgt cgccgtccac catcgccttc cgcgtgtagt cccagcggtc
    11161 gccgtcgatc cacgggccgt cctgctgagc gccgccgccg cccatctgat cgcggcacag
    11221 gtacaggtat ccgcgcttcg caccgctggt ccggacgttg tcgtccgcca ccgccagtcg
    11281 cgtcaggtcg aggtccttgc ccttggccgt cgcgtcgccc tcgccgcacg ccgccagtcc
    11341 gaccgccagt gccgccagcg ctcccccgat gcagatgatg cccctcacga tccctcctcc
    11401 ggttgctgcg cgccaccttc ccgctcgcgc gtatgcgcca cgtgagaggt gcgtaagagg
    11461 atctgaagaa ctactcgcgg acgatcagga acacgcccgc ggccagcagc gccacgccga
    11521 tcacgcgacc gacgctgagc ggcttctcgt cgagcccgaa ggcgccgagc cggtccatca
    11581 cgaccgagaa cgagagctgg cccgtgatcg tggccgccgt gaggccgccc gcgccgagtg
    11641 tgcgcacgct caccagcacc gtcgtcacgt acgcggctcc cagcaagccg cccgtgaggt
    11701 agtaccagcg caggtcgccg atggcggcga ggtccacctt cgagttgctc agcagggtga
    11761 tgccgatcag cgcggtcgtc cccaccgcga acgagatcga cgccgccgcg aacgtgcccg
    11821 ccgccttccc gagcgccgag ttgatcggcg cctgcatcgc cacgagtccg cccgcgcagg
    11881 ccgtgagcag caccgcgacc ccgggcttca cttcgccgcc agccagttct cgccggagcc
    11941 ggcgtccacg gccagcggcg ggtcgagctc gtaggcgtcg gccatctcgc gcaccacgat
    12001 ctccgacgcg gcgtcgacct ccgccaccgg cgcctcgaac agcagctcgt cgtggatctg
    12061 gaggaccagg cgggtggaca gccccgcgtc gcgcagcgcg ttctcgcagc ggaccatcgc
    12121 gaccttgatg atgtcggccg cgctgccctg gatcggcgtg ttcacggcga gccgctcgcc
    12181 gagcgagcgc gtctgccact tgcgcgagcg cagctcgggg atgcggcgct tgcggccgaa
    12241 gatcgtggtc acgtagccgc tctcggtcgc gtcggcgatc gtcgagtcga tgaactggcg
    12301 gaccttgggg aagcggtcga ggtagcgcgc gatgaactcg ccggcctcgt cctggggaat
    12361 gtcgagccgg tcggcgagcc cgtacgccga gagcccgtac acgatcccga agttgatcat
    12421 cttggccttc gagcgcgtgc ccggatcgat gtccttcggc tcgacgccga acatctcggc
    12481 ggcggtggcc gtgtgcacgt cctcgccgcg ggcgaagatg tccttcagca cctcttcgcc
    12541 acacacgtgc gcgagcacgc gcagctcgac ctgcgagtag tcggcgctga tcagccggtg
    12601 gccctcctcg gcgacgaagc aggcgcggat ctcgcggccc agctcggttc ggatcgggat
    12661 gttctggagg ttcgggtcgg tgctcgagag ccgaccggtg gtggtcgagg tctggttgaa
    12721 ggtcgtgtgc aggcggctgc gctggtccac cagcagcggc agcgcgtcga ggtaggtcga
    12781 cttgagcttc gagagctcgc gccagcgctc gatcttcggg atgatctcgt gctcgccgcg
    12841 gatcgcctgg agcacgcgcg cgtcggtcga gaaaccggtc ttgccacggc gcttgcgcga
    12901 gagctggagc ttctcgaaca ggatctcgcc gagctgctgg ggcgagccga tcgtgaactc
    12961 cgtgccggcc agctcgtgga tctcgccctc cagggtctcg acctgctcct gcaccttggc
    13021 gctgatctcg gcgagcttgg cggtgtcgag cttcacgccc gcgcgctcga ggtccacgag
    13081 cacgtccacc agcggaagct cgacctcctc gaacaggcgg gtcaggccct ccttctcgag
    13141 ctccgcgcgc tggatctcgg ccacggcgcg ggtcacgacg gcgcgctcgg cgaggccgtt
    13201 gccggttccc tgcccctcgg acggggggtc gatcttcgcg ccgagcccct cgcgctccgc
    13261 cagctcgtgg agcgggtacg cgcggcccgc cgggtcgagc aggtaggcgg cgacgatcgt
    13321 gtcgtgctgg agcggcgggg cctggctcgg ctcctcgctc gcggccaggg acttccagtc
    13381 gtgggccacg accgggcgct cgccccaggc cgcggcgatg gcggcgaggc tctcggcctc
    13441 gccggtcagg accgtctcgc cgccggcata tgcggcgaat cgcaacggcg gctggggaga
    13501 ggcggcctcg acatcgaggg agagctcgag cggggcggct tcgtcgggac tttcctgtcc
    13561 tggagggaca gtgtccccct gggacaggac ggtcgggtca tcctccggga cggggcgctc
    13621 cgcgacgagg gtcgcgagcg tcccgtccaa ctccctaagt gccgccgttg ggccctccac
    13681 cgctcgtgcg gagagcaccg tcgccgcgat ctcccgcggc gccgccgcgt cctcccccag
    13741 cgcctcctcc agccgctcca ggggcgcacg cagctcgaac tcgcggaaca cctcgcgcag
    13801 gcgcccgcgg tcgggctcgt cggcgaggca cgactcgagg tcgatgtcga gctcgacgtc
    13861 gcgcacggcg gtggcgagct gcttcgacat ccgggcgtcg tcggcgtggt tggtcaggtt
    13921 ctccttgcgc ttggcgccgg agatctcgtc cacgctcgcg agcaccgtct ccagggagcc
    13981 gaagcgctgg agcagctcgg aggcggtctt gtcgccgatg cccgggacgc ccgggatgtt
    14041 gtccgaggtg tcgcccttga ggccgtagaa gtcggggatc agctccggcg cgatgccgta
    14101 gcggtcgatc acggcctggc ggtcatagac cttggtctcg gtgatgccgc ggctcgtggc
    14161 catgaccttc acctcgccgc ggccgtcctc gcgcggctcg atgagctgga acgcgtcgcg
    14221 gtcgccggtc acgatcgtta cgtcgatcga ctccgcgcgg gcgcgctccg cgagcgtggc
    14281 gatcacgtcg tcggcctcgt agcccggcac cttcacgttg cggtagccga acgcctcgac
    14341 cagcggctcg aagtgcggcc actgctcgcg cagcaggtcg ggccgcgact tgcgctgtgc
    14401 cttgtagtcg gcggagacct cgtcgcggcc cgacatgccc gcgtcccaga ccacgacggt
    14461 cgacttcgga ccgtgctcgg tgaggatctt cacgagcatc gaggcgaacc cgaagatcgc
    14521 gttggtgggc tgccccttcg aggtcgcgat cgtctccggc agcgcgaaga aggcgcgata
    14581 gacgaggctg ttcccgtcga tcaggaagat ctcgcgggtc tcgtcggcgg tcaccggtgg
    14641 atcttaggcg cgtgcctcga tgaccagcat gtcctcctcg cgctccccgg gagcgagcag
    14701 gccgcggcgc tcgagcagct cggcgcgggc ggagaccagc gggccgagcg ggccgcgctc
    14761 ccgcgccacc acctcggcgc ggagcccgcc ggcgcgcagc gcggcgaggg tctcctcctc
    14821 cccggtcagc gaggacacga ccatcaggac gcggccgccg ggcgacaggt gctcggccac
    14881 gccggccgcg aagcgatcca cgagtgcccg cccgtcgaag cccccctccc acgcccgcgc
    14941 ggcgccggcg gtcgggatct cgtcgccggg cacgtagggc gggttggcgg tgatcaggtc
    15001 gaagcgctcc ccgctcaccg gtccgaacag gtctccggag agcgcgcgca cgcggcggcc
    15061 gttc
//
LOCUS       DASVPL010000204         3959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_378482, whole genome shotgun sequence.
ACCESSION   DASVPL010000204 DASVPL010000000
VERSION     DASVPL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378482"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VF517_10790"
     CDS             complement(<1..918)
                     /locus_tag="VF517_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053469.1"
                     /translation="MTPALRLAAALIAMAALAPAVAHAARPGQSFPLGDGRVVTSGPA
                     PGRALACAPPAASPSPAGRLPWIAGRRWTPSRRPVVDGALVWPLASFELGGAGRVTDF
                     AGNGLPVLGRTGVFPPPAGDDAQPFATGAAALAEHDVRGSFSERPRVRGAACIDPSLP
                     VAIASDGVPIVAPFDGRGRDLVAREVTDRCGGRTDAAGLYRYRGAAPCLGRRASGRAH
                     SGLVGWARDGFAVYGPRGAHGRPLRSRDLDACHGHRHRVGSSKASTYHYHLTPDFPYS
                     IGCFRGRPAPGWTITQRGTGDEPPAGPQAP"
     gene            complement(927..1418)
                     /locus_tag="VF517_10795"
     CDS             complement(927..1418)
                     /locus_tag="VF517_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8053470.1"
                     /translation="MAGKHDASPPPRRGVYMISVAAELAGMHPQTLRIYESRGLIEPE
                     RTSGGTRRYSADDVERLRRIQELTSELGMNLAGVEKVFELEQELDRMRERMRRLERQA
                     ERVQQEMAEEIEKVRKSFRAELVPYQAPGAALIPARDAKAGRVRIPIQRQPRGRDGGS
                     PRV"
     gene            complement(1418..2548)
                     /gene="dnaJ"
                     /locus_tag="VF517_10800"
     CDS             complement(1418..2548)
                     /gene="dnaJ"
                     /locus_tag="VF517_10800"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8053471.1"
                     /translation="MPAVKDPYSVLGVDKKATQEDIKKAYRKLARQYHPDRNPDDKGS
                     EERFKEVQQAYSMVGDPEKRKEYDSGGGLFGGFDPGGFRTGGGGGPGVGGFGDILSDL
                     FGGAARGGGARTANRPERGRDLETEVHVSFEQAMEGAQVQVTVPMTASCRTCAGTGAK
                     PGTSPSVCSRCQGRGVESEGQGLFSISQPCSKCGGTGTEIKDPCPTCHGTGNTRQVKR
                     YRANIPAGVRDGSRVRLAGKGEPGRRGGPAGDLYVITRVGDSPVFRRKGEHLEVDVPI
                     TIVEAIRGATVEVPTLNGTKRIRVPAGTQHGTVQRLRGEGPPRLGGKGRGDIHYRLAV
                     DIPRSLDRKQKKAVDQLAEVMDGNPRERLFTGAAGNAAGEEE"
     gene            complement(2559..3167)
                     /locus_tag="VF517_10805"
     CDS             complement(2559..3167)
                     /locus_tag="VF517_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX8053472.1"
                     /translation="MTRHTPNEAAVREQLKQRDDNGAAAEQAAAPEPSEEAGQQAEES
                     QAQEAPQEEAPQDPLAQAEAQRDEYLDLARRTQAEFENYRKRNAQMASQAGERAKVGL
                     LRELLPVVDNLERALAAAAEDEGPLAEGVKLVLADLQGVLARAGVEALVPVGEKFDPT
                     IHEALSMRPEEGAESGIVVEVVEKGYSTRDSVIRPARVVVSA"
     gene            complement(3167..>3959)
                     /locus_tag="VF517_10810"
     CDS             complement(3167..>3959)
                     /locus_tag="VF517_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8053473.1"
                     /translation="AGDVKDVLLLDVTPLTLGIETKGGVMTKLIERNTTIPTRKSEVF
                     STAEDNQPSVEVHVLQGEREMANNNKSLGKFQLTGIPPAPRGMPQIEVTFDIDANGIL
                     NVSAKDLGTGKEQKIEIKAGSGLSEDEVERMVKDAEAHAEDDRRQRELVEARNNAENA
                     AYQAEKQLGDLGDAVDASSKETIEAAIKDLRENLESDDPALLREKTEAVQTAFHAVSE
                     QMYAQAAQEQAESAPGGNGASADGDGSVSDEEVVDAEVVEGEGRE"
BASE COUNT          439 a         1486 c         1430 g          604 t
ORIGIN      
        1 gggcgcctga gggcccgcgg gcggctcgtc gcccgtcccc cgctgcgtga ttgtccagcc
       61 cggggcgggg cggccgcgga agcagccgat cgaatacggg aagtcggggg tcaggtggta
      121 gtggtaggtg ctggccttcg agctgccgac ccggtggcgg tggccgtggc aggcgtcgag
      181 gtcgcggctg cgcaacggcc tcccgtgggc gccgcgcggc ccgtagaccg caaagccgtc
      241 gcgcgcccag ccgaccagcc cggagtgcgc acgaccgctg gcacgccgcc cgaggcacgg
      301 cgccgcgccg cgatagcggt acaggccggc ggcgtcggtc cggccaccgc agcggtcggt
      361 gacctcgcgc gcgacgagat cgcggccgcg gccgtcgaac ggcgcgacga tcgggacccc
      421 gtcggaggcg atcgcgaccg gcaggctcgg gtcgatgcac gccgctccac gcacgcgcgg
      481 tcgctcgctg aacgagcctc gcacgtcgtg ctcggcgagg gcggcggcgc cggtggcgaa
      541 cggttgcgcg tcgtcgccgg ccgggggcgg gaagactccc gtgcggccca gcacgggcaa
      601 cccgttgccc gcgaagtcgg tgacgcggcc cgctccgccg agctcgaacg acgcgagcgg
      661 ccacaccagc gcaccgtcca cgacaggccg ccgcgacggc gtccagcgcc ggcccgctat
      721 ccacggcagc cggcccgcgg gggacgggga ggcggcgggc ggcgcgcacg cgagcgcccg
      781 cccgggcgcc ggtcccgacg tgaccacgcg accgtcgccg agcggaaagc tctggccggg
      841 cctcgcggcg tgcgccacgg cgggggccag cgcggccatg gcgatcagcg cggccgcgag
      901 tcgtagggcc ggcgtcacgc ggccgctcaa acgcggggag agccgccgtc gcggccgcgc
      961 ggctggcgct ggatcgggat gcgcacccgg ccggccttcg cgtcgcgcgc ggggatcagc
     1021 gccgcgcccg gcgcctggta gggcacgagc tcggccctga acgacttgcg gaccttctcg
     1081 atctcctcgg ccatctcctg ctgcacgcgc tcggcctggc gctcgaggcg gcgcatgcgc
     1141 tcgcgcatcc ggtcgagctc ctgctcgagc tcgaagacct tctcgacgcc ggcgaggttc
     1201 atgcccagct cggaggtcag ctcctggatc cggcgcaggc gctcgacgtc gtcggctgag
     1261 tagcgccgcg tgccgccgct cgtgcgctcc ggctcgatca ggccgcgcga ctcgtagatg
     1321 cgcagcgtct gcgggtgcat gcccgcgagc tccgcggcca ccgagatcat gtagacgccg
     1381 cggcgcgggg gcggcgatgc gtcgtgcttg ccggccatca ctcctcctct ccggcggcgt
     1441 tcccggcggc accggtgaag agccgctcgc gcgggttgcc gtccatgacc tcggcgagct
     1501 ggtcgaccgc cttcttctgc ttcctgtcga gggaccgcgg aatgtccacc gcgagccggt
     1561 agtggatgtc gccgcgaccc ttcccgccga gccgcggcgg gccctcgccg cgcagccgct
     1621 ggacggttcc gtgctgcgtc ccggcgggca cgcggatgcg cttggtcccg ttcagcgtgg
     1681 gcacctcgac ggtcgcgccg cggatggcct ccacgatcgt gatcggcacg tccacctcga
     1741 ggtgctcgcc cttgcgccgg aagaccgggg agtcgcccac gcgggtgatc acgtagaggt
     1801 cgccggccgg accgccgcgg cggccgggct cgcccttgcc ggcgagccga acgcggctgc
     1861 cgtcgcgcac gccggcgggg atgttcgcgc ggtagcgctt gacctggcgg gtgttgccgg
     1921 tgccgtggca ggtcgggcac gggtccttga tctcggtgcc ggtaccgccg cacttcgagc
     1981 acggctgcga gatcgagaac agcccctgtc cctcggactc gacgccgcgc ccctggcagc
     2041 gcgagcagac gctcggcgac gtgcccggct tcgcgccggt acccgcgcag gtgcggcagg
     2101 acgcggtcat cggcacggtc acctgaacct gcgcaccctc catcgcctgc tcgaacgaga
     2161 cgtgcacctc ggtctcgagg tcgcgcccgc gctcgggccg gttggccgtg cgcgcgccgc
     2221 cgccgcgggc ggccccgccg aagaggtcgg agaggatgtc gccgaagccc ccgacgcccg
     2281 ggccgccgcc cccgcccgtg cggaacccgc ccggatcgaa gccgccgaag aggccgccgc
     2341 cggagtcgta ctccttgcgc ttctcggggt cgccgaccat cgagtacgcc tgctggacct
     2401 ccttgaatcg ctcctccgag cccttgtcgt cggggttgcg atcggggtgg tactggcgcg
     2461 cgagcttgcg gtacgccttc ttgatgtcct cctgcgtcgc cttcttgtcg acaccgagga
     2521 cgctgtatgg gtccttgacc gcgggcatgg ctcctcctct aggcggagac caccacgcgg
     2581 gccggtcgga tcacgctgtc acgcgtgctg tagcccttct cgaccacctc gacgacgatg
     2641 cccgactcgg cgccctcctc gggtcgcatc gacagcgcct cgtggatcgt gggatcgaac
     2701 ttctcgccga ccgggacgag cgcctcgacg cccgcgcgcg cgagcacgcc ctggaggtcg
     2761 gccagcacga gcttgacccc ctcggccagc ggaccctcgt cctcggcggc cgccgcgagc
     2821 gcccgctcga ggttgtccac gaccgggagc agctcgcgca gcagcccgac cttcgcccgc
     2881 tcgccggcct gcgaggccat ctgggcgttg cgcttgcggt agttctcgaa ctcggcctgc
     2941 gtgcgccggg cgaggtcgag gtactcgtcg cgctgggcct cggcctgcgc gagcgggtcc
     3001 tggggagcct cctcctgcgg agcctcctgc gcctgcgatt cctcggcctg ctgccccgcc
     3061 tcctcggacg gctcgggagc ggcggcctgc tcggccgccg ccccgttgtc gtcccgctgc
     3121 ttgagctgct cgcgcacggc ggcctcgttc ggcgtgtgcc gcgtcattac tcgcggccct
     3181 cgccctcgac cacctcggcg tcgacaacct cctcgtcgct caccgagccg tcaccgtccg
     3241 ccgacgcgcc gttgccgccc ggagccgact cggcctgctc ctgggccgcc tgcgcgtaca
     3301 tctgctcgga caccgcgtgg aacgccgtct gcacggcctc ggtcttctcg cggagcagcg
     3361 ccgggtcgtc ggactccagg ttctcccgga ggtccttgat cgcggcctcg atcgtctcct
     3421 tcgaggacgc gtcgaccgcg tcaccgaggt cgccgagctg cttctcggcc tggtaggccg
     3481 cgttctcggc gttgttgcgg gcctcgacca gctcgcgctg gcgacggtcg tcctcggcgt
     3541 gcgcctcggc gtccttgacc atccgttcga cctcgtcctc ggacaggccc gagccggcct
     3601 tgatctcgat cttctgctcc ttgccggtgc cgaggtcctt cgcggacacg ttgaggatgc
     3661 cgttggcgtc gatgtcgaac gtgacctcga tctgcggcat gccgcgcggc gccgggggga
     3721 tcccggtgag ctggaacttg ccgagcgact tgttgttgtt ggccatctcg cgctcgccct
     3781 ggagcacgtg gacctcgacg ctcggctggt tgtcctcggc cgtcgagaac acctccgact
     3841 tgcgcgtcgg gatcgtggtg ttgcgctcga tgagcttcgt catcacgccg cccttggtct
     3901 cgatgccgag ggtcagcggg gtcacgtcga gcaggaggac gtccttgacg tcgccggcc
//
LOCUS       DASVPL010000205         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_63639, whole genome shotgun sequence.
ACCESSION   DASVPL010000205 DASVPL010000000
VERSION     DASVPL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63639"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3023)
                     /locus_tag="VF517_10815"
     CDS             complement(<1..>3023)
                     /locus_tag="VF517_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX8053474.1"
                     /translation="EAPAGAGYTPGEAAPGDGAVAAGPTAPLPAIPLILSARTGAALA
                     AQAERLRGRLLERTELEPLDVAYSLATGRARFERRAALVAADRDELIACLDALAAGEL
                     PAAATLGAPRPGKSAFLFTGQGAQWAGMGRDLYEAYPVFAQALDAACAELDAQLGRSL
                     RELVFADEGSPEAELLGRTENTQAALFALEVALYRLFESFGLRPDYLVGHSSGELTAA
                     HVAGVLSLPDAAALVAARGRLMGALPEGGAMLAVEADEDELAPTLGEQLSIAAINGPR
                     ALVVSGAQQAVEECEAHWRAQGRKTSRLDVSHAFHSPLMEPMLDEFRAVAAGLGFEAP
                     QMAVVSNLTGALATDELTDPGYWVRHVREAVRFADGVRELERRGVTRFVELGPDGVLT
                     AMARGCVSEEHAERSLFAAAMRAEREGPRTLAACAGAIEVAGLGLDWHAYFAGRGAER
                     VDLPTYAFQRERYWLEGADGPGDLGAAGLDAVDHPMIGAALPMAGGGGWLFTDGWSLA
                     THPWLADHAVFETVIAPGTAFAELALRVGGEVGCGAIEELTIEAPLLLPEHGTVHLQV
                     AVGEPDEEGRREIAIHSRAAATGEDGAAPTDWTRHASGLLAVAPQDGGEAAADRLASE
                     AWPPPGAEPVDIDGLYDRLAASGYAYGPAFQGVTAAWRRGDEVFAQIGLDDENADRAV
                     HFGIHPALFDASFHAVLGVLDEELEPGQVPLPFSWAGVRLLRAGAPSLRVSVARTGAD
                     TLRLTALDEDGVPALVVDTLTTRTLDAGRLESARSAGPDSLFAIEWTEASVASPNGHP
                     TRFAVLGDGGGDAVHDRHADMTALLAALDAGSPPPAAVVAFIRSADGEPAAAARAVAA
                     EALSLLQGWLADEGLAGTRLVIATHGAVAVDGEQPDLAQAPVAGLVRSAQSEHPEHFQ
                     LVDLDGSDPPWTAILASDEPQLAVRGGTVLAPRLARAHGLAPPPDASGGWRLAAERKG
                     TLEDLALVSSDRGSVPLEEGEVRVAVRAAGLNFRD"
BASE COUNT          361 a         1110 c         1214 g          338 t
ORIGIN      
        1 cgtcgcggaa gttgaggccg gccgcccgca ccgcgacgcg cacctctccc tcctcgagcg
       61 gcacgctgcc gcggtcggag gacacgagcg cgaggtcctc gagcgtgccc ttgcgctcgg
      121 cggcgagccg ccagccgccg ctcgcgtccg gcggcggggc caggccgtgg gcgcgggcga
      181 gccgcggcgc caggacggtg ccgccgcgca ccgcgagctg cggctcgtcg ctcgcgagga
      241 tggcggtcca cggcgggtcg ctgccgtcga ggtccacgag ctggaagtgc tccgggtgct
      301 cggactgcgc gctgcggacc aggccggcga ccggcgcctg ggcgaggtcg ggctgctcgc
      361 cgtccaccgc gacggcgccg tgcgtggcga tcacgaggcg cgtgccggcg agcccctcgt
      421 cggcgagcca gccctggagc agcgacagcg cctcggcggc cacggcgcgg gcggcggcgg
      481 ctggctcccc gtcagcggag cgtatgaacg cgaccacggc cgcgggcggc gggctgccgg
      541 cgtcgagggc cgcgaggagc gccgtcatgt cggcatggcg gtcgtgcacc gcgtcgccgc
      601 cgccgtcgcc gagcacggcg aagcgggtcg ggtggccgtt cggcgaagcc accgaggcct
      661 cggtccactc gatcgcgaac aaggagtccg ggccggcgct gcgcgcggac tcgagccggc
      721 cggcatcgag cgtgcgcgtg gtgagcgtgt cgaccacgag ggcggggacg ccgtcctcgt
      781 cgagcgccgt gaggcgcagc gtgtcggcgc cggtacgcgc gaccgagacg cgcagcgacg
      841 gggcgcccgc gcgcagcagg cggaccccgg cccacgagaa cgggagcggc acctgacccg
      901 gctcgagctc ctcgtcgagc acgccgagca cggcgtggaa ggaggcgtcg aagagcgcgg
      961 gatggatccc gaagtgcacc gcgcggtcgg cgttctcgtc gtcgaggccg atctgggcga
     1021 acacctcgtc cccgcgccgc cacgccgccg tcacgccctg gaacgcggga ccgtaggcgt
     1081 agccgctcgc ggccaggcgg tcgtagaggc cgtcgatgtc gacaggctcg gctccgggcg
     1141 gcggccacgc ctcgctcgcg agccggtcgg ccgcggcctc gcctccgtcc tgcggcgcga
     1201 cggcgagcag cccgctcgcg tggcgcgtcc agtccgtcgg cgcggcgccg tcctcgcccg
     1261 tcgcggccgc gcgcgagtgg atcgcgatct cgcgccgtcc ttcctcgtcc ggctcgccga
     1321 cggccacctg aaggtggacg gtgccgtgtt ccggcagcag cagcggcgcc tcgatcgtca
     1381 gctcctcgat cgctccgcat ccgacctcgc cgcccacccg cagggccagc tcggcgaacg
     1441 ccgtccccgg cgcgatcacg gtctcgaaca ccgcgtggtc ggccagccac gggtgcgtgg
     1501 cgagcgacca gccgtcggtg aacagccacc cgccgccgcc ggccatgggc agcgccgcgc
     1561 cgatcatcgg gtgatcgacg gcatcgagcc cggcggcgcc gaggtcgcca gggccgtcgg
     1621 cgccctccag ccagtagcgc tcgcgctgga aggcgtaggt cggcaggtcg acgcgctctg
     1681 ctccgcggcc ggcgaagtag gcgtgccagt cgagcccgag ccctgcgacc tcgatcgccc
     1741 cggcgcaggc ggcgagcgtg cgcgggccct cgcgctcggc gcgcatggcg gcggcgaaca
     1801 gggagcgctc ggcgtgctcc tcggagacgc agccgcgcgc catcgccgtc agcacgccgt
     1861 cggggccgag ctcgacgaag cgggtcactc cgcggcgctc gagctcgcgg acgccgtcgg
     1921 caaagcggac cgcctcgcgc acgtggcgga cccagtagcc ggggtcggtc agctcgtcgg
     1981 tggcgagcgc gccggtcagg ttcgagacga ccgccatctg tggggcctcg aagccgaggc
     2041 cggcggcgac ggcgcggaac tcgtcgagca tcggctccat caggggtgag tggaaggcgt
     2101 ggctgacgtc gaggcgcgat gtcttgcgcc cctgcgcgcg ccagtgggcc tcgcattcct
     2161 cgaccgcctg ctgcgcgccg ctgacgacga gcgcgcgcgg tccgttgatc gccgcgatcg
     2221 agagctgctc gccgagggtg ggtgcgagct cgtcctcgtc ggcctcgacc gccagcatcg
     2281 cgccgccctc gggcagcgcg cccatcagcc ggccgcgcgc ggcgacgagc gcggcggcat
     2341 ccgggagcga gagcactccg gcgacgtggg ctgcagtgag ctcgccgctc gagtgcccga
     2401 ccaggtagtc ggggcgcagg ccgaaggact cgaacaggcg gtagagggcg acctcgagcg
     2461 cgaacagagc ggcctgggtg ttctcggtgc gcccgagcag ctcggcctcg ggcgagccct
     2521 cgtcggcgaa gacgagctcg cggagcgagc gcccgagctg tgcgtcgagc tcggcgcagg
     2581 cggcgtcgag cgcctgcgcg aagaccgggt aggcctcgta gaggtcgcgg cccatgccgg
     2641 cccactgggc gccctggccg gtgaacagga acgcgctctt gccggggcgc ggcgcgccga
     2701 gcgtggcggc ggcgggcagc tcgccggcgg cgagcgcgtc gaggcaggcg atcagctcgt
     2761 cgcggtcggc ggcgaccagt gccgcgcggc gctcaaagcg ggcgcggccg gtcgcgagcg
     2821 agtaggcgac gtcgagcggc tcgagctcgg tgcgctcgag caggcggccg cgcaggcgct
     2881 cggcctgggc ggcgagggcg gcgccggtgc gcgcggagag gatcagcggg atggcgggga
     2941 ggggtgcggt cgggccggct gcgaccgcac cgtcgcccgg cgcggcttcg ccgggcgtgt
     3001 accccgcacc ggccggcgcc tcc
//
LOCUS       DASVPL010000206        13924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_219672, whole genome shotgun sequence.
ACCESSION   DASVPL010000206 DASVPL010000000
VERSION     DASVPL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219672"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1424)
                     /locus_tag="VF517_10820"
     CDS             complement(138..1424)
                     /locus_tag="VF517_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8053475.1"
                     /translation="MGEPGGADLVYVPTFFGAIEHYWEYHATARFFDRLSECGRLVLF
                     DRRNTGMSDVSLDGGYALEDQIDDVLAVMDAAGVERAGVFAQLEGCMMASLFAGTHPD
                     RVSSLVLYAPFARLTRAPGYEIGLTREERDEQIIGPSLAQWGEGTRGIPIAPSLADDP
                     EFVEWFGKLERLTSGPGLMQRMMDLNSETDVRSILPEVRVPTLVMSRPATEFFDHAHA
                     VYVADHIPGARLLELPGNDIFVAGGDVEPLISEIEEFFTGTRRRRPPDRVLATVLFTD
                     IVGSTERAAQLGDSGWRQLLDRHNDLVRRHLDRHQGREVKTIGDGFLATFDGPARGVR
                     CAAEIADEVRRLGIEIRAGLHTGECELVGDDVAGMAVNIGARVSSLAGPSEVLVSGTV
                     KDLVVGSGLEFDDRGEHELKGVPGAWRLYGVAATGG"
     gene            complement(1492..2661)
                     /locus_tag="VF517_10825"
     CDS             complement(1492..2661)
                     /locus_tag="VF517_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8053476.1"
                     /translation="MATPQEVAGRYRIEGRLGAGGMSTVFRAQDQVLERPVAVKLLAE
                     HLAEDEAFVARFRREALAAARLQHPNIVQVFDSGQDAQSGRHYIVMEFVEGPSCADLL
                     RQHRMFDAEDTVRIVRDACHGLDYAHRAGVIHRDVKPGNLLVASETGTTKLADFGIAK
                     AAEQTRITQVGSVLGTAAYLSPEQAAGEETGTASDIYSLGVCTYQFLAGRLPHEYGSL
                     TELALKQQQETAVPITEFRQDVPPELDRAVRASLERDPNRRYSTALEMATALEAGLRG
                     ESTAATERLDTDATRALAAGEATSILARPPATRVGVPPPQRLEPRPPVDPEPRREKKK
                     RNPLAWVIGILIALLLGAAIALALGLDKGIGENIREDNSSQQIDELIRYVRENRE"
     gene            complement(2661..3194)
                     /locus_tag="VF517_10830"
     CDS             complement(2661..3194)
                     /locus_tag="VF517_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053477.1"
                     /translation="MRRLVPTALVVLAAVAAGCGDDEPKIPRQDAASLRTDLLEAKRR
                     LRPLRCDDLETNSFPKLERRAAKLPEGSDVRETLEEGIDHLRSLVEADCAARREEKEE
                     TTDTTEPETTTAPPETTTAPPETTQPPKTDTQPTTPTTPTTPTTPDTQPQPDNGGGQT
                     VPQGTVKPGRGKKKDKG"
     gene            complement(3191..3955)
                     /locus_tag="VF517_10835"
     CDS             complement(3191..3955)
                     /locus_tag="VF517_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8053478.1"
                     /translation="MTVHSPDNVEAQRVALISDVHGNLPAFQAVLADIERAGVDARWC
                     LGDLVGYGAQPDECVALARESCDVCLIGNHDLVVIDKIEITEFSHNAAVAAVWTREHI
                     AQESVDYLSGLASSGQGGELGLYHASPRDPVWEYVLSAPQAGQCMDAMEPRVGAVGHS
                     HVALWFSRTDGGAVSGDQARGGTELDLSQGDWIVNPGGVGQPRDGDPRAAWLLLDLGT
                     WTATWHRVEYEIDAAARAIEQAGLPDVLSKRLYLGQ"
     gene            3944..5176
                     /locus_tag="VF517_10840"
     CDS             3944..5176
                     /locus_tag="VF517_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8053479.1"
                     /translation="MHGHRVGLPVAVAIAVVAAGAATVAFRPRSGVIDSAPASAQAYF
                     SPSELERSRDYSGPQRALGLGGLAVTGGALALIALRPPRRVRRALERAGARPLLGAAG
                     AGAGLSIALVVVSLPLGAVRHSRAEEFGLSTQDWGSWAADVAKAAGIEALLAGAGAAL
                     LIGLMRRFPRNWWLPASGAVVAISAAFVWLSPIVIEPIFNKFTPLAQGDLRREVLDLA
                     DRSGVEVGEVYRIDASRRTTGANAYVGGLGHTKRVVLYDNLIEGFSPAEVRSVVAHEL
                     GHVKHDDVPKGLLWIAIVAPAGLFLIQRLTERLAPDAERAGPSTLPAAVFSLALVSFV
                     LGLAGNAVSRGVEARADAYALRLTDDPAGFIALERRLVTQNLGDPDPPGLLVALFGTH
                     PPAMERIGFALTYAREKR"
     gene            complement(5173..5475)
                     /locus_tag="VF517_10845"
     CDS             complement(5173..5475)
                     /locus_tag="VF517_10845"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX8053480.1"
                     /translation="MPDEPAPERQYNIQTTPEAMMGVYANFANVSHSDYEFTITYARV
                     DHEVEDVEVPGVVVTRVNISPRFMRELIDAMEDNYSKWQARERIRELPEFPGEAEG"
     gene            5511..5918
                     /locus_tag="VF517_10850"
     CDS             5511..5918
                     /locus_tag="VF517_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX8053481.1"
                     /translation="MKTIVLAVGRLRPPYADDVQHYQKLLARHTRLELIEVREDQSLD
                     GRIPERAFVVLLAADGREYDSVGFSGFLEERRQEGRDLCFVVGGPRGLDLDRCDLRLS
                     LGRMTLPHQLARVVVLEQLYRAHKILAGEPYHY"
     gene            5920..7533
                     /gene="argS"
                     /locus_tag="VF517_10855"
     CDS             5920..7533
                     /gene="argS"
                     /locus_tag="VF517_10855"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX8053482.1"
                     /translation="MPRSDPIAELAATVQAAAEELGGGKSIAGAKLDRPPRPDFGDFS
                     SNAPMLLAPTLGEPPRAVAERLGALVGERLDGSLDRVEVAGPGFLNLFLSDDWFRAAL
                     DGARDAGERYGGGSVAVAEKVQVEFVSANPTGPLTVASGRHAAWGDALSRMLEFAGHD
                     VEREYYVNDHGSQVRRFGESIQARARGEEPPEDGYRGAYVERLAEELPDAAGAPDPWE
                     LARAGVELMVASVRATLERFRVDFDSFFHESSLHAKGKIEQVVELLRERGHVYTHEDA
                     LWLRTTDFGDDKDRVLQRGSGEYTYFAADIAYHLDKRERGFDRAINLWGADHHGYVRR
                     MLAAWEAVGGEPGTLELLIMQLVNLLERGQRVQMSKREGEFVTLDDLIDDIGVDAARF
                     FLLQRSHDTTLDLDLARAREQSQENPVYYVQYAHARIASILRKAKAEESTPAADAGSA
                     PLHPSERGLVKQLLELPGEVGVAAERRAPHRLTEYALETARSFSAFYRDCQVVGGDHE
                     AFRLELCSLTKLVLARSLDLLGVEAPEHM"
     gene            complement(7540..8277)
                     /locus_tag="VF517_10860"
     CDS             complement(7540..8277)
                     /locus_tag="VF517_10860"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX8053483.1"
                     /translation="MIAATDVDTTVFAAFAVGLVSFVSPCVLPLVPGYLSAISGVSVA
                     ELAEGERRAQVLAPALLFCLSFSVMFVALGMTATGLGQTLLDHRETLRQVAGVVIVLM
                     GLLFIATLFVTRLNRQWRPEALLRRASTGGPIVAGLAFAVAWLPCTGPTLGAILTAAS
                     TQDTVAKGGVLLAFYALGLAVPFVLCAVAFTRVTGIFRFFRDHWTAITVVSGVVLVAM
                     GVLLYTNELTELNAWLQDRGVNGISDL"
     gene            complement(8274..8726)
                     /locus_tag="VF517_10865"
     CDS             complement(8274..8726)
                     /locus_tag="VF517_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEX8053484.1"
                     /translation="MRRIAALLCAVLALGVPAAALAACPQTTVADLEDEVMCPVCGTS
                     LATAGDAPLAVQERRLIDGLVKQCRSKQQIKDVLVAQYGQSVLAEPSDGGFRTATYLI
                     PALALAGGLVGVVLAARRWRRRRPRPPAAAPDSPDSALLDADMGRYDL"
     gene            complement(8723..9277)
                     /locus_tag="VF517_10870"
     CDS             complement(8723..9277)
                     /locus_tag="VF517_10870"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8053485.1"
                     /translation="MKRFLTPVSIAALVAVAALFALLVYGVLQGGEKSGIDEALADGR
                     RPEAPALSLPRLDRPGKLSVDDHRGKVVVLNFWASWCAPCRDEAPLLERWHRRIAAQG
                     GTVLGVDALDATGDAREFVRRFGITYPNVRDVSGEHLRPFGISAYPETFVLDRRGRIA
                     ALVRGPVTEEFLAREIPPLLREPS"
     gene            9423..10652
                     /locus_tag="VF517_10875"
     CDS             9423..10652
                     /locus_tag="VF517_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053486.1"
                     /translation="MAIRRAKISARTLRCALAACAVLAAAPAAADATTLTLRPEATSL
                     AGWTVTPSGTTHHEALNDAVTAPSPPLTATDYVSSALSTTQFLKMSLTTASLPSGENV
                     TGATAWGYLQTGSGRTIIMWVMSGSTTLGSVTIPAGSPAGWYQLALTVPLTQSQVDAM
                     EIGFTSASGSGTANFVYATYVQVDTTNPTPPPPPPPPPPPPVDPPVDPAPVDPAPVDP
                     PADPGPTTPPSDPPASPGPSPTTSPSEPTTVPTDSGTPVSTDPQSPVVVPVTPVQPTV
                     DPVTDVVKPALAIAGTNPTATPAGDVPVTVECAADMAGGCEGVLWLEDPLSVRSDSQI
                     SAARRGTGRFSKNKKYKLRAGQRKTVPVRLDRRTYRKFKNKRSFKVTVVAQQKDPNGQ
                     VVTQRRTVRVFNRKKKR"
     gene            10735..10807
                     /locus_tag="VF517_10880"
     tRNA            10735..10807
                     /locus_tag="VF517_10880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10768..10770,aa:Gly,seq:ccc)
     gene            10842..11255
                     /locus_tag="VF517_10885"
     CDS             10842..11255
                     /locus_tag="VF517_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053487.1"
                     /translation="MWDWADAWLLQSITYAGRRGDLRAVIHHADYINVDIPTRDDIER
                     SVNRLAAAGLVEPSGLRLRATRQGRKLVRQSGRWSQGLREVPPRLTAKLRDEVAFPSQ
                     PTDWSLSEEDWQAAYDRYYPPEKRAAAIPRPHDSA"
     gene            complement(11291..12547)
                     /locus_tag="VF517_10890"
     CDS             complement(11291..12547)
                     /locus_tag="VF517_10890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053488.1"
                     /translation="MRAVVTPALSLLRSERRARWFFAALAQSALGSGAGYVALLVVAY
                     ERLHSPWAISLVLLADFAPAMVLGPLLGAAADRWSRRRCAVLADAVRAVAFVGVALAG
                     SLEATVAFALLAGTGTALFRPAAMAALPSLVPEGRSAAATSLYSATSQAGFVVGPAIA
                     ALVLLAVGPEQLLLANGATFAISAAILARLDFGEPVAAAAGSPATIRSLLADAREGIG
                     ALAGMPLLRLIVAASTIGALLGAVYNVVELPFATDALGSDASGYAALAAAYGIGYVGG
                     SLRGSGGGDAARLKRGFVRGLALLGAGGLAIGVSPAIGPAVAAFAVAGCGNGLAVVHE
                     RLLLQSEVAPRLHGRSFAAIEWLAAWGFAAGFLLAGVLMALTGPRELMLLIAAAEIAL
                     AAVAAVALLRPRAAPGIGARYGEQVT"
     gene            complement(12765..13808)
                     /locus_tag="VF517_10895"
     CDS             complement(12765..13808)
                     /locus_tag="VF517_10895"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX8053489.1"
                     /translation="MRAITVIPGQPQSAQLDEIAEPVRDADELLVQGIALGVCGTDRE
                     IVRGEYGWAPPGRERLVLGHESLGRVLEAPADSGFHEGDLVVGIVRRPDPEPCRCCAA
                     GEWDMCQNGLYTERGIKEIDGYGAERYTLEPEFAVAVPAQLEPVAVLLEPTSVVAKAW
                     EQIDRISQRACSPGGRVLVTGAGPIGLLAGLLGKQRGLDVHVLDLEEDGPKPALTKAL
                     GAEYHVGSVEQCPEADIVIEATGVGQLVFDVMQHAAPGAIVCLTGVAATGRKLSADPG
                     AINNELVLRNEVVFGSVNAARRHYEQAADALKRADAQWLDGLISRRVALADWEAALEK
                     RDDDVKVVIELTG"
BASE COUNT         1924 a         5005 c         5123 g         1872 t
ORIGIN      
        1 ccgccatgcg ggggcggtgc ctccggtcct ccagtgctca tggcgcgaaa gcgtaactcg
       61 ccccgcggac gagggtgcga gtccatttca tagtgctata ggaccaacaa atggactctc
      121 gccccctccc gggggcgcta accgccggtc gccgccaccc cgtacagccg ccaggctccc
      181 ggcacgccct tcagctcgtg ctcgccgcgg tcatcgaact cgagtcccga gccgaccacc
      241 aggtccttga ccgtccccga caccagcacc tcgctgggcc ccgccagcga ggacacccgc
      301 gcgccgatgt tcacggccat gccggccacg tcgtcgccga ccagctcgca ctcgcccgtg
      361 tgcagcccgg cccggatctc gatgccgagc cggcgcacct cgtcggcgat ctcggccgcg
      421 cagcgcacgc cgcgcgccgg gccgtcgaac gtcgcgagga agccgtcgcc gatggtcttg
      481 acctcgcgcc cctggtggcg atccaggtgc ctgcgcacca ggtcgttgtg gcggtcgagg
      541 agctggcgcc agccggagtc gccgagctgc gccgcgcgct cggtcgatcc gacgatgtcg
      601 gtgaagagca cggtcgcgag cacgcggtcg ggcgggcgcc gccggcgcgt gccggtgaag
      661 aactcctcga tctcgctgat gagcggctcg acgtcgccgc ccgcgacgaa gatgtcgttg
      721 ccggggagct cgagcagccg cgcgcccggg atgtggtcgg ccacgtagac ggcgtgcgcg
      781 tggtcgaaga actccgtcgc gggccgcgac atcacgagcg tcgggacgcg gacctcgggc
      841 aggatcgagc ggacgtcggt ctcggagttc aggtccatca tgcgctgcat cagtccgggc
      901 ccggaggtga ggcgctcgag cttgccgaac cactcgacga actcggggtc gtcggcgagg
      961 ctcggtgcga tcgggatgcc gcgcgtaccc tcgccccact gtgcgaggct cggcccgatt
     1021 atctgctcgt cccgctcctc gcgcgtgagg ccgatctcgt acccgggcgc gcgcgtgagc
     1081 cgcgcgaacg gggcgtagag aacgagcgac gagacccggt cggggtgggt cccggcgaac
     1141 agcgaggcca tcatgcagcc ctcgagctgg gcgaacaccc cggcgcgctc gacgcccgcg
     1201 gcgtccatca cggcgagtac gtcgtcgatc tggtcctcga gggcgtagcc gccgtccagc
     1261 gagacgtccg acatgccggt gttgcggcgg tcgaacagca cgagccggcc gcactcgctc
     1321 agccggtcga agaagcgtgc ggtggcgtgg tactcccagt agtgctcgat cgccccgaag
     1381 aacgtcggga cgtagacgag gtcggccccg cccggctcgc ccacaacctg ataggcgagg
     1441 tggaggtcgc cgttcctggc gtagcgggtc ttcggacgcc gcgccgcggc gctactcccg
     1501 gttctcgcgg acgtagcgga tcagctcgtc gatctgctgc gaggagttgt cctcgcggat
     1561 gttctcgccg atgcccttgt cgagcccgag cgccagcgcg atcgccgctc ctagcagcag
     1621 cgcgatcagg atgccgatca cccaggcgag cgggttgcgc ttcttcttct cgcggcgcgg
     1681 ctccgggtcg accggcggcc gcggctcgag ccgctggggc ggggggacgc cgacgcgggt
     1741 ggcgggcggg cgggcgagga tgctcgtcgc ctcgccggcg gccagagcgc gcgtcgcgtc
     1801 cgtgtcgagc cgctcggtgg ccgcggtcga ctcgccgcgc aggcccgcct cgagggcggt
     1861 ggccatctcg agcgccgtgg agtagcggcg gttcgggtcg cgctcgaggc tcgcgcgcac
     1921 agcgcggtcg agctcgggcg ggacgtcctg gcggaactcc gtgatcggaa ccgccgtctc
     1981 ctgctgctgc ttgagggcga gctcggtgag cgagccgtac tcgtgcggga gccggccggc
     2041 gaggaactgg taggtgcaga cgccgagcga gtagatgtcc gacgcggtgc cggtctcctc
     2101 gcccgcggcc tgctcgggcg agaggtaggc ggcggtgccg agcaccgagc cgacctgcgt
     2161 gatccgcgtc tgctcggccg ccttcgcgat gccgaagtcc gcgagcttcg tcgtgccggt
     2221 ctccgaggcg acgaggaggt tccccggctt cacgtcgcga tggatcacgc ccgcgcggtg
     2281 cgcgtagtcg agcccgtggc aggcgtcgcg cacgatccgg acggtgtcct cggcgtcgaa
     2341 catgcggtgc tggcggagca ggtcggcgca cgacggtccc tcgacgaact ccatgacgat
     2401 gtagtggcgc ccggactgcg cgtcctggcc ggagtcgaag acctgcacga tgttcgggtg
     2461 ctggagccgc gccgcggcca gcgcctcgcg gcggaagcgg gccacgaaag cctcgtcctc
     2521 ggcgaggtgc tcggcgagca gcttgaccgc cacgggccgc tcgagcacct ggtcctgcgc
     2581 gcggaagacc gtcgacatcc cgcccgcccc gagacggccc tcgatccggt agcgcccggc
     2641 gacctcctgt ggggtggcca tcagcccttg tccttcttct tgccccgacc gggcttgacg
     2701 gtgccctggg ggacggtctg gccgccaccg ttgtccggct gtggctgcgt gtccggagtg
     2761 gtcggcgtcg tgggcgtggt gggggtcgtc ggctgggtgt cggttttcgg cggctgggtg
     2821 gtctccgggg gagccgtggt ggtctccggc ggcgccgtcg tggtctccgg ctccgtcgtg
     2881 tcggtcgtct cctctttctc ctcgcgccgg gccgcgcagt cggcctcgac caggctgcgg
     2941 aggtggtcga tcccctcctc gagcgtctcg cgcacgtccg agccctccgg cagcttggcg
     3001 gcgcggcgct cgagcttcgg gaagctgttc gtctcgaggt cgtcgcagcg caggggacgc
     3061 agccgccgct tggcctcgag caggtcggtg cgcaggctgg cggcgtcctg gcgggggatc
     3121 ttcggctcgt cgtcaccgca gccggcagcg acggcggcga gcacgacgag ggctgtcggg
     3181 acgaggcgtc tcactgaccg aggtagagcc gcttcgagag tacgtcgggg agaccggcct
     3241 gctcgatagc acgcgcggcc gcgtcgatct cgtactcgac gcggtgccag gtcgcggtcc
     3301 aggtcccgag gtcgagcagg agccacgcgg cgcgcgggtc gccgtcgcgc ggctggccga
     3361 cgccgcccgg gttcacgatc cagtcgccct gtgagaggtc gagctcggtg ccgccgcggg
     3421 cctggtcgcc gctcaccgcc ccgccgtcgg tgcgcgagaa ccagagcgcc acgtgcgagt
     3481 gccccacggc gcccacgcgc ggctccatgg cgtccatgca ctggcccgcc tgcggagccg
     3541 agagcacgta ttcccagacc gggtcgcgcg ggctcgcgtg gtagaggccg agctcgccgc
     3601 cctgcccgct cgacgcgagc cccgacaggt agtccacgga ctcctgcgcg atgtgctcgc
     3661 gtgtccagac cgccgccacg gcggcgttgt gggagaactc ggtgatctcg atcttgtcga
     3721 tcacgaccag atcatggttg ccgatgaggc acacgtcgca cgactcgcgc gcgagcgcga
     3781 cgcactcgtc gggctgggcc ccgtagccaa cgaggtcgcc caggcaccag cgcgcgtcga
     3841 cacccgcccg ctcgatgtcg gcgagcaccg cctggaaggc gggcaggttg ccgtgcacgt
     3901 cggagatcag ggcgacgcgt tgcgcttcta cattgtcagg ggaatgcacg gtcatcgtgt
     3961 tgggctgccg gtcgcggtgg cgatagccgt tgtcgccgcc ggagcggcga cggtcgcctt
     4021 ccggccgcgg agcggcgtga tcgactcagc gccggccagc gcgcaagcct acttcagccc
     4081 atccgagctc gaacggagtc gcgactacag cggcccccag cgcgcgctgg gcctgggcgg
     4141 gctcgccgtc acgggtggcg cgctggcgct gatcgcgctg cggccgccgc ggcgggtgcg
     4201 ccgcgcgctc gagcgggcgg gcgcgcggcc gctgctcggg gcggcggggg cgggggcggg
     4261 gctgtcgatc gcgctcgtgg tcgtctcgct gccgctgggg gcggtgcgcc actcgcgggc
     4321 cgaggagttc gggctctcga cccaggactg gggctcttgg gccgccgacg tggccaaggc
     4381 cgccgggatc gaggcgctgc tcgcgggcgc cggggcggcc ctcctgatcg gactcatgcg
     4441 gcgcttcccg cgcaactggt ggctgcccgc ctcgggggcc gtcgtggcca tcagcgcggc
     4501 gttcgtctgg ctctccccga tcgtgatcga gccgatcttc aacaagttca cgccgctcgc
     4561 gcagggtgac ctgcgccgcg aggtgctcga tctggccgac cgctcgggcg tggaggtggg
     4621 ggaggtctac cggatcgacg cgagccgccg gacgaccggc gccaacgcct acgtgggcgg
     4681 gctcgggcac accaagcgcg tcgtgctcta cgacaatctg atcgagggct tctcgccggc
     4741 ggaggtgcgg tcggtggtcg cgcacgagct cgggcacgtg aagcacgacg acgtgccgaa
     4801 ggggctgctg tggatcgcga tcgtcgcgcc cgccgggctg ttcttgatcc agcggctgac
     4861 cgagcgactg gcgcccgacg ccgagcgcgc cgggccgagc acgctgcccg ccgccgtgtt
     4921 ctcgctcgcg ctcgtctcgt tcgtcctcgg gctggccggg aacgccgtct cgcgcggcgt
     4981 cgaggcgcgg gccgacgcct acgccctgcg gctcaccgac gacccggcgg gcttcatcgc
     5041 gctcgagcgg cgactcgtga cccagaacct cggcgacccc gacccgcccg gcctgctggt
     5101 ggcgctgttc ggcacgcacc cgcccgcgat ggagcggatc ggcttcgcgc tcacctacgc
     5161 gcgggagaag cgctagccct cggcttcgcc ggggaactcg ggcagctcgc ggatccgctc
     5221 gcgcgcctgc cacttcgagt agttgtcctc catggcgtcg atcagctcgc gcatgaagcg
     5281 cggggagatg ttcacgcgcg tgacgacgac cccgggcacc tcgacgtcct cgacctcgtg
     5341 gtcgacgcgc gcgtaggtga tggtgaactc gtagtcggag tggctgacgt tcgcgaagtt
     5401 cgcgtagacg cccatcatcg cctcgggcgt cgtctggatg ttgtactggc gctcgggcgc
     5461 cggctcgtcg ggcacggggc tagtatcgcg gaaatcggga ccggccaccc atgaagacga
     5521 tcgtcctcgc ggtcggacgc ctccggccgc cctacgccga cgacgtccag cactaccaga
     5581 agctcctcgc ccggcacacg cggctcgagc tgatcgaggt gcgcgaggac cagtccctgg
     5641 acggtcgcat ccccgagcgg gcgttcgtgg tgctgctggc cgcggacggc cgcgagtacg
     5701 actccgtcgg gttcagcggc ttcctggagg agcgccgcca ggagggccgc gacctctgct
     5761 tcgtggtcgg gggcccgcgc gggctcgatc tcgaccgctg cgacctgcgc ctctcgctcg
     5821 gccggatgac gctgcctcac cagctcgcgc gcgtggtcgt gctcgaacag ctctaccgcg
     5881 cgcacaagat cctcgccggg gagccatacc attactgacg tgccccggag cgaccccatc
     5941 gcggagctcg ccgcgacggt ccaggcggcg gccgaggagc tgggcggggg caagtcgatc
     6001 gccggtgcca agctcgaccg cccgccgcgc ccggacttcg gcgacttctc cagcaacgcg
     6061 ccgatgctgc tcgcgccgac gctcggcgag ccgccgcgcg cggtcgcgga gcggctgggc
     6121 gcgctcgtgg gcgagcggct cgacggctcg ctcgaccgcg tcgaggtggc cgggccggga
     6181 ttcctgaacc tgttcctctc ggatgactgg ttccgcgcgg cgctggacgg agcgcgcgac
     6241 gccggcgagc gctacggagg cgggtcggta gcggtcgctg agaaggtcca ggtggagttt
     6301 gtgagcgcga acccgaccgg acccctcacg gtggccagcg ggcggcacgc ggcctggggc
     6361 gacgcgctct cgcgcatgct cgagttcgcc ggccacgacg tggagcgcga gtactacgtc
     6421 aacgaccacg gctcgcaggt gcggcgcttc ggcgagtcga tccaggcgcg ggcgcgcggc
     6481 gaggagccgc ccgaggacgg ctaccgcggc gcctacgtcg agcggctggc ggaggagctg
     6541 cccgatgccg ccggcgcgcc ggacccgtgg gagctggcgc gggccggagt cgagctgatg
     6601 gtcgcctccg tgcgcgccac gctcgaacgc ttccgcgtgg acttcgacag cttcttccac
     6661 gagagctcgc tgcacgcgaa ggggaagatc gagcaggtcg tcgagctgct gcgcgagcgc
     6721 gggcacgtct acacgcacga ggacgcgctc tggctgcgca cgaccgactt cggcgacgac
     6781 aaggaccgcg tgctccagcg cggcagcggc gagtacacct acttcgcggc cgacatcgcg
     6841 taccacctcg acaagcgcga gcgcggcttc gatcgcgcga tcaacctctg gggcgccgac
     6901 caccacggct acgtgcggcg gatgctcgcc gcatgggagg ccgtgggcgg cgagcccggc
     6961 acgctcgagc tcttgatcat gcagctcgtc aacctgctcg agcgcgggca gcgcgtgcag
     7021 atgtcgaagc gcgagggcga gttcgtgacc ctcgacgacc tgatcgacga catcggcgtg
     7081 gacgccgccc gcttcttcct cctccagcgc agccacgaca ccacgctcga cctcgacctc
     7141 gcacgcgcgc gcgagcagtc gcaggagaat cccgtctact acgtgcagta cgcgcacgcg
     7201 cgcatcgcga gcatcctgcg caaggcgaag gcggaggaga gcacgcccgc ggcggacgca
     7261 gggagcgcgc cgctgcaccc gtccgagcgc gggctggtga agcagctgct cgagctgccc
     7321 ggcgaggtgg gtgtcgcggc cgagcgccgc gcgccgcacc gcctgaccga atacgcgctc
     7381 gagacggcgc gcagcttctc ggcgttctac cgcgactgcc aggtggttgg cggcgaccac
     7441 gaggccttcc gcctcgagct gtgctcgctc acgaagctcg tgctcgcccg gtcgctcgac
     7501 ctgctcgggg tcgaggcgcc ggagcacatg tagcgtctac tagaggtcgc tgatgccgtt
     7561 cacgccacgg tcctggagcc aggcgttcag ctcggtcagt tcgttggtgt agagcaggac
     7621 gcccatcgcg accagcacca cgcccgagac gacggtgatc gcggtccagt ggtctcggaa
     7681 gaagcggaag atgcccgtca cgcgggtgaa cgcgaccgcg cagagcacga acgggacggc
     7741 gaggccgagc gcgtagaagg cgagcagcac gccgcccttg gcgaccgtgt cctgcgtgct
     7801 ggcggcggtg aggatcgccc cgagggtggg gccggtgcac ggcagccacg cgacggcgaa
     7861 cgcgaggccg gcgacgatcg ggccgccggt cgaggcgcgc cgcagcagcg cctcggggcg
     7921 ccactggcgg ttgagccgcg tgacgaacag cgtggcgatg aagagcagcc ccatgaggac
     7981 gatcacgacg ccggcgacct ggcgcagggt ctcgcggtgg tcgagcaggg tctggccgag
     8041 cccggtcgcg gtcatgccga gggccacgaa catcacgctg aagctcaggc agaagaggag
     8101 cgccggggcc agcacctgcg cgcgccgctc gccctcggcc agctcggcga ccgagacgcc
     8161 cgagatcgcc gagaggtagc ccggcacgag cgggagcacg cacggcgaga cgaacgagac
     8221 gaggcccacg gcgaacgcgg cgaagacggt ggtgtcgacg tcggtggcgg cgatcacagg
     8281 tcgtagcggc ccatgtccgc gtcgagcagg gcgctgtccg gcgagtcggg cgcggcggcc
     8341 gggggccgcg ggcggcggcg gcgccagcgg cgcgcggcca ggaccactcc gaccaggccg
     8401 ccggccagcg cgagcgccgg gatcaggtac gtggcggtgc ggaacccgcc gtcgctcggc
     8461 tcggccagca cggactgccc gtactgcgcc accagcacgt ccttgatctg ctgcttgctc
     8521 cggcactgct tcacgagccc gtcgatcagg cggcgctcct ggacggccag cggcgcgtcg
     8581 ccggcggtgg cgagcgacgt gccgcagacc gggcacatga cctcgtcctc gaggtcggcg
     8641 acggtggtct gcgggcacgc ggcgagcgcc gccgcgggaa cgccgagggc gagcacggcg
     8701 cagagcagtg ccgcgatgcg cctcacgacg gctcccgcag gagcggcggg atctcgcgcg
     8761 cgaggaactc ctcggtgacc gggcccctca cgagcgcggc gatccgcccg cggcggtcga
     8821 gcacgaacgt ctccggatag gcggagatcc cgaacggccg gaggtgctcg cccgagacgt
     8881 cgcgcacgtt cgggtaggtg atcccgaaac ggcgcacgaa ctcgcgcgcg tcgccggtgg
     8941 cgtcgagggc gtcgacgccg agcacggtgc cgccctgcgc cgcgatccgg cggtgccagc
     9001 gctcgagcag cggcgcctcg tcgcggcagg gagcgcacca cgaggcccag aagttgagca
     9061 ccacgacctt cccgcggtgg tcgtcgaccg acagcttgcc ggggcggtcg aggcgcggca
     9121 ggctgagcgc cggagcctcg ggccggcggc cgtcggcgag cgcctcgtcg atgccgctct
     9181 tctctccccc ctggaggacg ccgtagacga gcagggcgaa cagcgcggcc accgccacca
     9241 gcgcggcgat cgagaccggt gtgaggaacc gcttcacgcg ccgtcaaggg taaagctggg
     9301 ctctggccgg aatctaagcg cgcattgcga gggtcacggg agcctcggat atcggtccag
     9361 aagggggcaa ccgggcgccc gcgggctaaa gagtggcgcc gcgccaggcg acaaggaggt
     9421 agatggccat ccggcgtgcc aagatttccg cgaggacgct gcgctgcgcg ctggcggcct
     9481 gcgccgtcct ggccgccgcc ccggccgcgg ccgacgccac cacgctcacc ctgcggcccg
     9541 aggcgacctc gctggccggc tggacggtca cgccttcggg aaccacgcac cacgaagcgc
     9601 tgaacgacgc cgtcacagcg ccgagccccc cgctgaccgc gaccgactac gtcagcagcg
     9661 cgctctcgac gacccagttc ctcaagatga gcctgacgac ggcgagcctg ccgagcggcg
     9721 agaacgtcac cggcgccacc gcctggggct acctccagac cggctcgggg cgcacgatca
     9781 tcatgtgggt catgtccggc tccaccacgc tcggctcggt cacgatcccc gcgggcagcc
     9841 cggccggctg gtaccagctc gcgctcaccg tgccgctgac gcagtcgcag gtcgatgcga
     9901 tggagatcgg cttcacctcc gcctccggaa gcgggaccgc gaacttcgtc tacgccacct
     9961 acgtgcaggt cgacacgacc aacccgaccc cgcccccgcc cccgcccccg cccccgcccc
    10021 cgccggtcga tcccccggtg gacccggccc cggtggaccc ggccccggtg gacccgccgg
    10081 ccgacccggg cccgacgaca ccgccgagcg atcccccggc cagcccgggc ccgagcccga
    10141 cgacctcgcc cagcgagccc acgacggtcc cgaccgattc gggcacgccg gtctccaccg
    10201 acccgcagtc gcccgtcgtc gtcccggtca cgccggtgca gcccaccgtg gaccccgtga
    10261 cggacgtggt caagcccgcc ctcgctatcg ccgggaccaa cccgaccgcg acgcccgcgg
    10321 gcgatgtccc cgtgaccgtg gagtgcgccg cggacatggc cggcgggtgc gagggtgtgc
    10381 tctggctcga ggatccgctc tcggtccggt cggacagcca gatcagcgcc gcgcgccgcg
    10441 gcaccggccg cttctccaag aacaagaagt acaagctgcg cgccggccag cgcaagaccg
    10501 tcccggtccg gctcgaccgc cgcacctatc gcaagttcaa gaacaagcgc tcgttcaagg
    10561 tgacggtggt cgcgcagcag aaggacccga acggccaggt cgtgacgcag cggcgcacgg
    10621 tccgggtgtt caaccggaag aagaagcgct agccgccccc gcgcgtcccg cgtggtgccc
    10681 acgcgcgtcc cgcgcggtgc ccacgcgcgt cccgcgcggt agattccgcg ctgcgcggcg
    10741 gtagctcagt tggtagagcg cgagcttccc aagctcgagg tcgcgggttc gaggcccgtc
    10801 cgccgctctg ccaccgacgg gtggccatag cctttggtgc catgtgggac tgggccgacg
    10861 cgtggttgtt gcaatcgatc acgtatgcgg gccggcgcgg cgacctgcga gccgtcatcc
    10921 accacgcgga ctacatcaac gtcgatatcc cgacccggga cgacatcgaa cgttcggtca
    10981 atcgtctcgc ggctgcaggt ctggtcgaac cgtccggtct tcgccttcgg gcgacgcgcc
    11041 agggtcggaa gctcgtccgc caaagcgggc gctggagcca ggggttgcgt gaggtaccgc
    11101 cgcgcctcac ggcgaagttg cgggacgaag tcgcgttccc ctcgcagccg acggactggt
    11161 cgctctccga ggaggactgg caagccgctt acgaccggta ctacccgcct gagaagcgtg
    11221 ctgccgcgat tccccgcccg cacgactccg cctgaggcct gctcgcgacg cggcgggagg
    11281 tccgtgctct ctacgtgacc tgctcgccgt agcgcgcgcc gatcccgggg gcggcgcgcg
    11341 gccgcagcag cgcgacggcg gcaaccgcgg cgagcgcgat ctccgcggcc gcgatcagca
    11401 gcatcagctc gcgcgggccg gtgagcgcca tcaggacgcc cgccagcagg aagcccgcgg
    11461 cgaagcccca ggcggcgagc cactcgatcg cggcgaagct gcgaccgtgc aggcgcggag
    11521 cgacctcgga ctggagcagc agccgctcgt ggacgacggc gagcccgttg ccgcagccgg
    11581 cgaccgcgaa cgccgcgacc gccggcccga tggccggtga gaccccgatt gcgaggccgc
    11641 ccgctccgag cagggcgagg ccgcgcacga acccgcgctt caggcgggcc gcgtcgccgc
    11701 cgcccgagcc gcgcagcgag ccgccgacgt agccgatccc gtaggccgcg gcaagcgccg
    11761 cgtagccgga ggcgtccgag ccgagcgcgt cggtcgcgaa cggcagctcg acgacgttgt
    11821 agacggcgcc cagcaacgcc ccgatcgtcg acgcggcgac gatcaggcgc agcagcggca
    11881 tcccggccag agcgccgatc ccctcacgcg cgtcggcgag cagcgagcgg atcgtcgccg
    11941 ggctcccggc ggcggcggcg acgggctcgc cgaagtcgag tcgggcgagg atcgccgccg
    12001 agatcgcaaa ggtggcgccg ttcgcgagca ggagctgctc ggggccgacc gcgagcagga
    12061 cgagcgcggc gatggccgga cccacgacga agcccgcctg ggaggtggcg ctgtagagcg
    12121 acgtggcggc ggccgagcgg ccctcgggga cgaggctcgg cagggccgcc atcgcggccg
    12181 gccggaacag ggccgtgccg gtgcccgcga ggagcgcgaa cgcgacggtc gcctccaggc
    12241 ttcccgcgag ggcgacgccc acgaacgcga cggcgcgcac ggcgtccgcg aggacggcgc
    12301 agcggcgccg cgaccagcgg tcggccgccg cgccgaggag tgggccgagc accatggccg
    12361 gggcgaagtc cgcgagcagc accaggctga tcgcccaggg ggagtggagg cgctcgtacg
    12421 ccacgacgag gagcgcgacg tagccggcgc cgctgccgag cgccgactgg gccagcgccg
    12481 cgaagaacca gcgcgcgcgc cgctcgctcc ggagcagtga gagcgcgggg gtcacgacgg
    12541 cgcgcaccgc cggagccaga tggcggtgcg cgtgtcgcgg tggtgaatgc gatcgcggcc
    12601 tgctcggccg caccgcgggc tagtacttgc cgtagggcat gttgcctcct gtcgatgagt
    12661 ccggacgagc atgcgatcca caccctcacg ggcccctggt agccgcaaga ggggcgcacg
    12721 gaggccggcc gctcccgccg ctcgtcggcc ggatggggaa atcgtcagcc ggtgagttcg
    12781 atgacgacct tgacgtcgtc gtcgcgcttc tcgagggcgg cctcccaatc cgcgagtgcc
    12841 acacgccgcg agatgaggcc atccagccac tgcgcgtccg cacgcttcag cgcgtcggcg
    12901 gcctgctcgt agtggcgtcg tgcggcgttc acggatccga ataccacctc gttccgcagc
    12961 acgagctcgt tgttgatggc gccgggatcg gcggacagct tgcggcccgt cgcggccacg
    13021 cccgtgaggc agacgatcgc cccgggtgcg gcgtgttgca tgacgtcgaa cacgagttgg
    13081 ccgactccgg tcgcctcgat gacgatgtcc gcctcggggc attgctccac ggatccgacg
    13141 tgatactcgg cgcccagggc cttcgtgagc gcgggcttcg ggccgtcctc ctccaggtcg
    13201 agcacgtgga cgtcgagtcc gcgctgcttg ccgagcagac cggcgagcag gccgatcggg
    13261 cccgcgcccg tcacgagcac cctcccgccc ggcgagcacg ctcgctgcga gatccggtcg
    13321 atctgctccc acgcctttgc cacgacgctc gtgggctcga ggagcacggc gacgggctcg
    13381 agttgcgctg ggaccgcgac cgcgaactcc ggttcgagcg tgtatcgctc ggcgccatat
    13441 ccgtcgatct ccttgatccc ccgctcggtg tagagaccgt tctggcacat gtcccactcg
    13501 ccggcggcgc agcaccggca cggctccggg tcgggccgcc ggacgatgcc gacgacaaga
    13561 tcgccctcgt ggaacccgct gtccgcgggc gcctccagca cccggccgag cgattcgtgg
    13621 cccagcacca gccgctctcg accgggcggt gcccagccgt actcgccgcg gacgatctcg
    13681 cggtccgtcc cgcagacccc gagcgcgatt ccctggacca gcagctcgtc cgcatctcgc
    13741 acgggctctg cgatctcgtc gagctgcgcg gattgcggct gacccgggat gacggtgatc
    13801 gcgcgcatgt ggaggatcta cccgagcgct ggggcctacc aactccatgg acgcggcgac
    13861 gttcgttcga ttacgtctcc cgaccaggcg ggtcatgcgg cgaaacgccg ccgcgcgcgg
    13921 tcga
//
LOCUS       DASVPL010000207         5266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_24601, whole genome shotgun sequence.
ACCESSION   DASVPL010000207 DASVPL010000000
VERSION     DASVPL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24601"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VF517_10900"
     CDS             complement(<1..827)
                     /locus_tag="VF517_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053490.1"
                     /translation="MQSAVARLVPAALVLAGLAYFATEAELLTTSTRQTALLVVALVV
                     LLHLALTVDPAWLLSAGIISTMFAGNWASLGLNASVGPHRVLLGAGILAVLLRAPPAR
                     DRPPLQLDHAHFALAAALAFAVVSAIWVGTLEGSRSQYVLLDQFGILPFGLFAIAPVA
                     FYTDRQRMILLGGLVVTGAYLSVTAVLEKLELYDLLVPSYIGDPSVGQHFGRARGPFA
                     ESAAMGLALYACAVAAAIALVVWRRPGPRALAAAVVVAAPVGLLLTVTRGVWVAGIAA
                     "
     gene            complement(834..1682)
                     /locus_tag="VF517_10905"
     CDS             complement(834..1682)
                     /locus_tag="VF517_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053491.1"
                     /translation="MPVEDITAPSYPPREQANFGRMPLLQALRRHWPLAVLPVLLLVG
                     AAIAIGLQREPEYESESRLNVGGLNLTQQSIEGYTTAVQALAVSYSRAIDATPVTEPA
                     ADELGLTPGEVADRISATPIQGSPVIRVIAKGDDAAETVRLADAAGDNLVDYAEELNS
                     GGSQSGRLLKRFRAASRELRAIGTDLRRARRRNSRNVERLEAREDIARLEQQVAGTLY
                     QNAQAGQATIGLVQKLAPATRAKSDRDDYLRQLVAGGAIGGLLIGIGLAVWRGNAVAR
                     RRLGAY"
     gene            1745..2893
                     /locus_tag="VF517_10910"
     CDS             1745..2893
                     /locus_tag="VF517_10910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8053492.1"
                     /translation="MLVVTNMLPGARRSGGEIVTQSVVDALFAAGHDVRVLGYRAPDD
                     TAADARGEACVGRRRIETRAAGLSAAGWMLRAVATRAPYTGAKWVSRDYRRAVAAGLA
                     ERPAALVVDHAGLLSVVPPAAALEVPMVFVGHNAEARMYEGLAAGSGSRPGRMVYRRE
                     ARRIGAYEAELARHARQVWALTPADADYFRSLNPSADVRTLEVASVLPEPVAGPEPEY
                     DVAIIGTWSWDANAQGLRWFADEVVPHLNGLRVEVAGRGAEWLDGRSPNVRVRGVVPD
                     AADFMARARVVAVPSVEGGGVQLKTLDGIACGVPLVSTTVGTRGLDGLPGSVAVADDP
                     AEFARQLERFAADPDRGRLRDEAVAWSRARRERLERDVADWVAEIARP"
     gene            2893..4110
                     /locus_tag="VF517_10915"
     CDS             2893..4110
                     /locus_tag="VF517_10915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8053493.1"
                     /translation="MRLAYVCSRYPSISHVFILREVLALRRAGVEIDPFTIRRDGEHE
                     LLTEVDREEDRRTHVIVPAPPLALVAAHARALLRSPSRYLATFGLALRLRGPGARSLL
                     WQVFYFGEAGLMWRECQRRGIRHIHAHHANVGSDVALLASSLGGPGWSWSFTMHGSTE
                     FFDVREHRLPQKVEHASFVVCVSRHGRSQLMAFVGPEHWDKLHVVHCGVDVDWFAAVD
                     RGGRDGSPEILTVGRTVPVKGQALLVESLAELHRRGLAARLTIVGDGPQLPGLRSLAE
                     RRGVAEHVDFVGPVGQDRLRAFYERADVFALPSFAEGLPIVLMEAMATGLPVVASRIT
                     GIPELVEEGVSGVLVVPGDGADLTGALERLLREPAERRAEMGAAGRAKVLAEFDVERT
                     ARELLAVYEERIG"
     gene            4110..>5266
                     /gene="asnB"
                     /locus_tag="VF517_10920"
     CDS             4110..>5266
                     /gene="asnB"
                     /locus_tag="VF517_10920"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX8053494.1"
                     /translation="MCGICGIAAADPGERPLDASLMTAMTDVITHRGPDEDGQRIEPG
                     VALGMRRLSIIDLPGSSQPMSNEDGSVWTVFNGEIYNFPELRRWLEGRGHALATRGDT
                     ETIVHLYEEHGPDFAKRLRGMYGIAVWDAPRRRLVLTRDRMGVKPLYLAHGPRGLAFA
                     SEVKSLIAGGLVEPRLDPAAAELFLAYGYVPGPATLFAGVRKLMPATTLVFEDGAIVS
                     EESYWDPWSDAPEGVGRDWREDEERLLELLRRSVEARMISDVPLGVMLSGGLDSSLIT
                     ALMAEANRGPVKTFSIGFAEDAGANELGDARRVAQRLGTEHHELETSAFDHPELLDEA
                     LWHLEEPVADLSFLAFLLLSRLARESVTVALSGQGADELLGGYRKHQVAAVG"
BASE COUNT          656 a         1886 c         2033 g          691 t
ORIGIN      
        1 gcggcaatcc cggcgaccca gactccgcgg gtgaccgtca gcagcagccc gaccggcgcc
       61 gcgaccacga ccgcagccgc cagcgcgcgc ggtcccgggc ggcgccagac caccagcgcg
      121 atggccgccg ccacggcgca cgcgtacagc gccagcccca tcgcggcgga ctcggcgaac
      181 ggcccgcgtg cgcggccgaa gtgctggccg accgaggggt cgccgatgta gctcggcacg
      241 agcaggtcgt acagctcgag cttctcgagg accgccgtga ccgacaggta cgcgccggtg
      301 acgacgaggc cgcccagcag gatcatccgc tggcgatcgg tgtagaacgc gaccggggcg
      361 atcgcgaaca gcccgaacgg caggatcccg aactggtcca gcaggacgta ctgcgacctg
      421 ctgccctcga gcgtcccgac ccagatcgcc gacacgacgg cgaacgcgag cgctgcggcc
      481 agcgcgaagt gggcgtggtc gagctggagc ggcggccggt cgcgggcggg cggtgcgcgc
      541 agcagcaccg cgaggatccc ggcgccgagc agcacgcggt gcggaccgac gctcgcgttc
      601 agcccgagcg aggcccagtt cccggcgaac atcgtcgaga tgatcccggc gctgagcagc
      661 caggcggggt cgacggtcag ggccaggtgg agcagcacga ccagcgcgac cacgagcagc
      721 gcggtctgcc gcgtggacgt ggtgagcagc tccgcctcgg tcgcgaagta cgcgagcccc
      781 gccagcacca gcgccgccgg gacgaggcga gcgacggcgc tctgcatcgc cggctagtac
      841 gccccgagtc gccgccgcgc cacggcgttg ccgcgccaga cggccagccc gatcccgatc
      901 aggagcccgc cgatcgcgcc gcccgcgacg agctgacgca ggtagtcgtc ccggtcgctc
      961 ttggcccggg tggccggcgc gagcttctgc acgagcccga tcgtcgcctg gccggcctgg
     1021 gcgttctggt agagcgtgcc ggccacctgc tgctcgagcc gggcgatgtc ctcgcgcgcc
     1081 tcgagccgct ccacgttgcg gctgttgcgc cggcgagcgc ggcgcaggtc ggtcccgatc
     1141 gcacgcagct cgcgcgaggc cgcccggaag cgcttcagca gccggccgct ctggctgcca
     1201 ccgctgttga gctcctcggc gtagtcgacg aggttgtcgc ccgcggcgtc cgcgagccgc
     1261 acggtctcgg cggcgtcgtc acccttcgcg atcacgcgga tcacggggct gccctggatc
     1321 ggcgtggccg agatgcggtc ggcgacctca cccggtgtca gcccgagctc gtccgcggcg
     1381 ggctcggtca cgggagtcgc gtcgatcgcc cgcgagtacg agaccgcgag cgcctggacg
     1441 gcggtcgtgt agccctcgat cgactgctgg gtgaggttga gcccgcccac gttgaggcgc
     1501 gactcgctct cgtactccgg ctcgcgctgg agcccgatcg cgatcgccgc gccgaccagc
     1561 agcaggacgg gcagcacggc gagcggccag tgccggcgca gcgcctggag cagcggcatg
     1621 cgcccgaaat ttgcctgctc acgcggggga tacgagggcg cggtgatgtc ctcgactggc
     1681 acggtggcgg gcagtgtaga gcgggtcggt ggccccatgt gctggcagaa tcggcgccgt
     1741 gccgatgctc gtcgtgacca acatgctgcc cggtgcgcgg cgcagcgggg gggagatcgt
     1801 gacccagagc gtggtcgacg cgctgttcgc cgcgggccac gacgtgcggg tgctgggcta
     1861 ccgcgcgccg gacgacacgg cagcggacgc gcgcggcgag gcgtgcgtgg ggcggaggcg
     1921 gatcgagacg cgcgccgccg gcctgagcgc cgccgggtgg atgctgcgcg cggtcgcgac
     1981 gcgcgcgccg tacaccggcg ccaagtgggt ctcgcgggac tatcgccggg cggtcgcggc
     2041 cgggctggcc gagcggcccg cggcgctcgt cgtggaccac gcggggctcc tctcggtcgt
     2101 tccgcccgcg gccgcgctcg aggtcccgat ggtcttcgtc ggccacaacg ccgaggcgcg
     2161 gatgtacgag gggttggcgg ccggctcggg cagccggccg ggccgcatgg tctaccggcg
     2221 cgaggcgcgc cggatcggcg cctacgaggc cgagctcgcc cggcacgcgc ggcaggtctg
     2281 ggcgctcacc cccgccgacg ccgactactt ccgctcgctg aacccatccg ccgacgtgcg
     2341 cacgctcgag gtggcatcgg tcctgccgga gccggtcgcg gggccggagc cggagtacga
     2401 cgtggcgatc atcggcacct ggagctggga cgcgaacgcg cagggcctgc gctggttcgc
     2461 cgacgaggtc gtgccgcacc tgaacggcct gcgggtcgag gtggccggcc gcggcgcgga
     2521 gtggctcgac gggcgcagcc cgaacgtgcg ggtccgcggc gtcgtgccgg acgcggccga
     2581 cttcatggcc cgggcgcggg tcgtggccgt gccgtcggtc gagggcggcg gcgtgcagct
     2641 caagaccctc gacgggatcg cgtgcggtgt gccgctcgtg tccaccacgg tcggcacgcg
     2701 cgggctcgac gggctccccg gctccgtcgc ggtcgccgac gacccggccg agttcgcccg
     2761 tcagctcgag cggttcgccg ccgacccgga tcgcggccgg ctgcgcgacg aggcggtcgc
     2821 gtggtcgcgc gctcgccgtg agcggctcga gcgcgacgtg gcggactggg tcgcggagat
     2881 cgcccgaccc tgatgcgcct cgcctacgtc tgcagccgct acccgtcgat ctcgcacgtc
     2941 ttcatcctcc gtgaggtgct ggcgctgcgc cgtgcggggg tcgagatcga cccgttcacg
     3001 atccgtcgcg acggcgagca cgagctgctc accgaggtcg atcgcgagga ggaccggcgc
     3061 acgcacgtga tcgtgccggc gccgccgctg gcgctcgtcg ccgcgcacgc gcgagctctg
     3121 ctgcgctccc cctcccgcta cctcgccacg ttcggcctcg cgctgcggct gcgcgggccg
     3181 ggcgcgaggt cgctcctctg gcaggtcttc tacttcgggg aggccggcct gatgtggcgc
     3241 gagtgccagc ggcgcggcat ccgccacatc cacgcccacc acgcgaacgt cgggtcggac
     3301 gtcgcgctgc tggcgtcgtc gctcggcggc cccgggtggt cgtggagctt cacgatgcac
     3361 gggagcaccg agttcttcga cgtgcgcgag caccggctgc cgcagaaggt cgagcacgcg
     3421 tccttcgtgg tgtgcgtgag ccggcacggg cgcagccagc tcatggcctt cgtcggtccc
     3481 gagcactggg ataagctcca cgtcgtccac tgcggcgtcg acgtcgactg gttcgccgcc
     3541 gtcgaccgcg gcggccgcga cgggtcgccg gagatcctga ccgtcgggcg gaccgtgccg
     3601 gtcaagggcc aggcgctgct ggtcgagtcg ctggccgagc tgcaccggcg cggcctggcc
     3661 gcgcggctga cgatcgtggg ggacggcccc cagctcccgg ggctgcgctc gctggcggaa
     3721 cggcgcggcg tggccgagca cgtcgacttc gtggggccgg tcggccagga ccggctgcgc
     3781 gcgttctacg agcgcgccga cgtgttcgcg ctgccgagct tcgccgaggg gctgccgatc
     3841 gtgctgatgg aggcgatggc caccggcctg ccggtcgtgg cctcgcgcat cacgggcatc
     3901 ccggagcttg tcgaggaggg cgtctccggc gtactcgtcg tgcccggcga cggcgccgac
     3961 ctgaccggcg cgctcgagcg cctgctgcgc gagcccgcgg agcggcgggc cgagatgggc
     4021 gcggcgggcc gcgcgaaggt gctcgcggag ttcgacgtcg agcggacggc gcgggagctg
     4081 ctcgcggtct acgaggagcg gatcggctga tgtgcgggat ctgcggcatc gcagccgcgg
     4141 acccgggcga gcgcccgctc gacgcctccc tcatgacggc gatgaccgac gtcatcaccc
     4201 atcgcggccc cgacgaggac ggccagcgga tcgagccggg ggtggcgctc gggatgcggc
     4261 ggctcagcat catcgacctg cccggcagct cgcagccgat gtcgaacgag gacgggtcgg
     4321 tgtggaccgt cttcaacggc gagatctaca acttccccga gctgcgcagg tggctcgagg
     4381 gccgcggcca cgccctggcg acgcgcggcg acaccgagac gatcgtgcac ctgtacgagg
     4441 agcacggccc cgacttcgcg aagcggctgc gcgggatgta cggcatcgcc gtgtgggacg
     4501 cgccgcggcg gcggctcgtg ctcacccgcg accgcatggg ggtcaagccg ctctacctcg
     4561 cgcacggccc gcgcgggctc gcgttcgcct ccgaggtcaa gtcgctgatc gccggcgggc
     4621 tcgtcgagcc gaggctcgac cccgcggcgg cggagctgtt cctcgcctac gggtacgtgc
     4681 cggggccggc gacgctgttc gcgggcgtgc gcaagctgat gccggcgacg accctcgtgt
     4741 tcgaggacgg ggccatcgtc tcggaggagt cgtactggga tccctggagc gacgcccccg
     4801 agggcgtcgg ccgcgactgg cgcgaggacg aggagcgcct gctcgagctg ctgcgccggt
     4861 cggtcgaggc gcgcatgatc agcgacgtgc cgctcggggt gatgctctcc ggcgggctcg
     4921 actcgagcct catcacggcg ctgatggcgg aggcgaaccg cgggccggtc aagacgttct
     4981 cgatcggctt cgcggaggac gcgggcgcga acgagctggg cgacgcgcgc cgggtggcgc
     5041 agcggctcgg caccgagcat cacgagctcg agacgagcgc gttcgaccac cccgagctgc
     5101 tcgacgaggc gctctggcac ctcgaggagc ccgtcgcgga cctctcgttc ctggcgttcc
     5161 tgctgctctc gcggctggcg cgcgagtccg tgaccgtcgc cctgtcgggc cagggcgccg
     5221 acgagctgct cggcggctac cgcaagcacc aggtcgcggc cgtcgg
//
LOCUS       DASVPL010000208         8673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_219935, whole genome shotgun sequence.
ACCESSION   DASVPL010000208 DASVPL010000000
VERSION     DASVPL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219935"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VF517_10925"
     CDS             <1..182
                     /locus_tag="VF517_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX8053495.1"
                     /translation="MCLGMNPDTLDPGERCASTSNRNFEGRQGRGGRTHLVSPEMAAA
                     AAIEGRFVDIREWDG"
     gene            182..742
                     /gene="leuD"
                     /locus_tag="VF517_10930"
     CDS             182..742
                     /gene="leuD"
                     /locus_tag="VF517_10930"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX8053496.1"
                     /translation="MDPIRTIEGGVSVLDRADVDTDQIIPKQFLKRVERTGFGEFLFY
                     DWAKEPGWELPKNPILAAGRNFGCGSSREHAPWALEDYGFRAIVAPSFADIFYSNCTK
                     IGLLPVILPEDDVRALMEAGEAEIDLDRQTVSFAGRELPFEIDAEIKHRLLNGLDDIG
                     VTLASAEAIDRYEQERERPGPVTTAL"
     gene            739..1488
                     /locus_tag="VF517_10935"
     CDS             739..1488
                     /locus_tag="VF517_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8053497.1"
                     /translation="MTQDWDASTYERVSGPQVEWAGQVIDRLELAGDETVLDAGCGSG
                     RVTRLLVERLPRGHVVAVDGAPSMVEHAREALGERATVIHSDLQELVLDGPVDAVFSN
                     AVFHWVPDHDLLFRRMHDALKPGGRMSVQCGGAGNVEGFHGEAAAVGEQAPFAEHLAG
                     WTGPWNFAGAEETKERLERAGFAEVETWLQPWPVHPDEPRAFIATVCLVHHLERLPED
                     LRERYVDAVAERIGERPTLDYVRLNIVARRD"
     gene            1481..2914
                     /locus_tag="VF517_10940"
     CDS             1481..2914
                     /locus_tag="VF517_10940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8053498.1"
                     /translation="MTERNALRALGGLVVLAAVLRFWRIGHQSFWLDEVFTAGLADKG
                     LWGMLKGVRETESTPHLYYLLAWLWAKVAGDGEGALRSLSALSGIATVPVAYLAAREL
                     FKPAAALTAAALVAVNPWFVWYSQEARAYALLTLIATASLLFFLRALKAESRRDLTLW
                     ALFSALAVLTHYFAVFLVGAEAVWLLWTMRERAARPVAALAAVGLALVPLAISQRGEG
                     HTAFIEDIALGSRIADLPKRFVTGELGTPTPLIGPLAGLLVVAGIALALCWRPERERA
                     FGLLALVTAAVLVPVLLAVVGPDLLLPRNAIAAYVPLAIVVAAGLATHRAGLAAAGVL
                     AALMLAVTIQISLNEHLQRDDWRAVAAALRDDPPGAGRAIVVTSEPQIAPLEHYLPGL
                     AEYPPRGAALNEIVTIEVTREGASREPVVPAGFTPAERRREPSFELVRLQSGSIQVPP
                     AQVLADTRLDDEAAALRFQPREYSRTP"
     gene            2911..3936
                     /gene="leuB"
                     /locus_tag="VF517_10945"
     CDS             2911..3936
                     /gene="leuB"
                     /locus_tag="VF517_10945"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX8053499.1"
                     /translation="MSAPRIAALPGDGIGPEIMAAARELLAALGEFEIEEHLVGGASI
                     DAHGTALTDAVLDACRQADAVLLAAVGGPKWDTTDPDKPRPEQGLLGLRKGLGLFANL
                     RPVRPSQALLDSSPLRRERIEGTDLLVVRELTGGLYFGERGRRDGTAFDTLVYSEEEV
                     DRICEVAFRFARTKVTSVDKANVLETSRLWRAVAERVAERHPDTPLDHLLVDNAAMQL
                     VARPADFDVIVTENMFGDILSDVAAMLTGSIGMLPSASVGAGGPGLFEPVHGSAPDIA
                     GTGSANPLAMFGSVAMMMRYGLGREDDAARVESAVDRALEEGLRTRDLGGEASTEEAT
                     GAVLSNL"
     gene            3951..4877
                     /locus_tag="VF517_10950"
     CDS             3951..4877
                     /locus_tag="VF517_10950"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX8053500.1"
                     /translation="MNTADLIWMNGEFVAWEDAKVHVLTHAMHYGTGVFEGIRCYETE
                     QGPAIFRHQDHLQRLRRSADLYYMDLPYSTEEIREVTHELVARNGLRSCYIRPVVHRG
                     YGPMGLDPLDNPVECFIAAWEWGSYLGDEGKTKGIRAKTSSWRRISPESQPPQAKACG
                     QYLNSVLAKVESRKAGFDEAILLDHKGVVCEGTGENVFVVRDGEIATPPMSASILDGI
                     NRRSIIEIARDLGYRVTERDIAREELALADEVFLTGTAAELTPMREIDDIEIGPPGEI
                     TLQIQRVFDDALHGRAERYREWLDLVQVPSKA"
     gene            4874..6481
                     /gene="cimA"
                     /locus_tag="VF517_10955"
     CDS             4874..6481
                     /gene="cimA"
                     /locus_tag="VF517_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX8053501.1"
                     /translation="MTQQVQIYDTTLRDGMQGEGMSLSVEEKLRVAHAVDELGWHFIE
                     AGFLSSNPKDEEFFRLLEHERFANVEVAAFGMTRRRGVAADEDPALRLLADSFAPVCT
                     LVGKTWKLHLEKVIKVDPDENLAMIADSVGFLREHGKRVVYDCEHFFDAYREDPEYAT
                     RCVRAAAEAGAEWVALCDTNGATLPDAIASNTHTVAMEFASACGIAIHTHDDAGCGVA
                     NSLLAVDAGARQVQGTVNGYGERCGNANLTTIVPDLQLKLGYECVSADQLAGLTEAAH
                     LIDEICNVSPNPNQPYVGRNAFAHKGGMHVAGVLSDARTFEHVDPAAVGSDRALLISE
                     LSGKGTVQARAEQAGLGISADEAVRVVDRVKELESRGFHFEAADGSFDLLIRRETGDY
                     EPLFRLESWRVIAEKREDGRVQTEATIKIWVGGERYLRTCEGNGPVNALDRALRAAIG
                     DVYPHLRDIELVNYKVRILDEDKGTGAVTRVLLDASDGTDTWGTIGVSENIIEASWEA
                     LVDSLEAGMLPRRSGHTRRAAGEPATP"
     gene            6478..7587
                     /locus_tag="VF517_10960"
     CDS             6478..7587
                     /locus_tag="VF517_10960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8053502.1"
                     /translation="MIPLARPVIGQREEELVLEVLRSGHLSLGPMLPRFEHAFADRLG
                     VGFASAVSSGTAGLHLAVRAAGVEPGDEVVTSPFSFVASANCVVYEGARPVFCDIDRR
                     TLNIDPAAAAAAVTERTAGLLPVHIFGYPADMPALERLAVDRGLWIVEDACEALGATH
                     GDGCVVGARGNLAVFAFYANKQMTTGEGGVVICPIEAHKARLDSERNQGRAPDMGWLD
                     HDRLGFNYRMSDLQCALGVAQLERLDGMLALRAAVAAHYREALAGIEGLELPCPDVGG
                     DMRGWVVYVVQLPPGVDRDATITALRERGIDSKPYLPAIHLMTFYRERFGHREGEFPV
                     CEDVAARSLALPFFPGLAESEVERIGENLRAVLYS"
     gene            7593..8621
                     /locus_tag="VF517_10965"
     CDS             7593..8621
                     /locus_tag="VF517_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8053503.1"
                     /translation="MRQALIAVMVLLLVPAGASAATASVDVHVSTGKGCSVSPESCVQ
                     AELDFTAAAGEANDVEVSTAGSSLVVRDPGTGVSAGSGCQQQGPKMVRCSPPEGPSMG
                     FVLISVVLGDGVDVLRNTTGIVSSATGGSGDDRLTGGTSSDTFDGGAGRDRLEGADGS
                     DYLQDTGPVEPDVLDGGAGYDKVDYSMRAARVTVDLRGPASPSGAAGEDDSLVGIEEG
                     HGGRGPDLLRAGADPVVLVGHGGADRIIGGFGNDLLIGNTGDDKLYGIGGSDRLVGGA
                     GNDGLRAGCGRSKLFGNAGNDRFDSRNGAANRVVGGAGRDVAVLDQLDTTQQVERRTR
                     QHIDACAL"
BASE COUNT         1220 a         3145 c         3117 g         1191 t
ORIGIN      
        1 cgatgtgcct cgggatgaac cccgacacgc tcgaccccgg cgagcgctgc gcgtcgacct
       61 cgaaccgcaa cttcgagggc cgccagggcc gcggcgggcg cacgcacctg gtgagcccgg
      121 agatggcggc ggcggcggcg atcgagggcc gcttcgtcga catcagggag tgggacggct
      181 gatggacccg atacgcacga tcgagggcgg cgtgtcggtg ctcgaccgcg ccgacgtgga
      241 caccgaccag atcatcccca agcagttcct caagcgggtg gagcgcacgg gcttcggcga
      301 gttcctgttc tacgactggg cgaaggagcc cggctgggag ctgcccaaga acccgatcct
      361 cgccgcggga cgcaacttcg gctgcggctc ctcgcgcgag cacgcgccgt gggcgctcga
      421 ggactacggc ttcagggcga tcgtcgcgcc ctcgttcgcc gacatcttct actcgaactg
      481 caccaagatc ggactgctcc cggtgatcct ccccgaggac gacgtgcgcg ccctgatgga
      541 ggcgggcgag gccgagatcg acctcgaccg ccagacggtg tcgttcgccg gccgcgagct
      601 gccgttcgag atcgacgccg agatcaagca ccgcctgctg aacgggctcg acgacatcgg
      661 cgtgaccctc gcgagcgccg aggcgatcga ccgctacgag caggagcgcg agcggccggg
      721 ccccgtcacc accgcgctgt gacccaggac tgggacgcct ccacctacga gcgcgtctcc
      781 ggcccgcagg tcgagtgggc cggccaggtg atcgatcgcc tcgagctcgc gggcgacgag
      841 accgtgctcg acgccggctg cggcagcggc cgggtcacgc ggctgctggt cgaacggctt
      901 ccccgcgggc acgtggtggc ggtcgacggc gcgccctcga tggtcgagca cgcgcgcgag
      961 gcgctcggcg agcgcgccac ggtgatccac tcggacctcc aggagctggt gctcgacggg
     1021 ccggtcgacg ccgtcttctc gaacgccgtc ttccactggg tgccggacca cgacctgctc
     1081 ttccgccgca tgcacgacgc gctgaagccc ggcggccgca tgtcggtcca gtgcggcggc
     1141 gcaggcaacg tcgagggctt ccacggcgag gccgcggccg tgggggagca ggcgccgttc
     1201 gccgagcacc tcgccggctg gaccgggccg tggaacttcg cgggcgccga ggagaccaag
     1261 gagcggctcg agcgcgctgg cttcgcggag gtggagacct ggctccagcc ctggccggtc
     1321 caccccgacg agccgcgcgc cttcattgcc acggtctgcc tcgtccacca cctcgagcgg
     1381 ctgccggagg acctccgtga gcgctacgtc gacgccgtcg ccgagcgcat cggcgagcgg
     1441 ccgacgctcg actacgtgcg gctcaacatc gtcgcgcgcc gtgactgagc gcaacgcgct
     1501 gcgcgcgctc ggcggcctcg tcgtgctggc cgcggtgctg cgcttctggc ggatcggcca
     1561 ccagagcttc tggctcgacg aggtcttcac tgccgggctc gcggacaagg ggttgtgggg
     1621 gatgctgaag ggcgtccgcg agaccgagag cacgccgcac ctctactacc tgctggcgtg
     1681 gctgtgggcg aaggtcgccg gcgacggcga gggcgccctg cggtcgctgt cggcgctgtc
     1741 cggcatcgcc accgtgccgg tcgcctacct cgcggcgcgg gagctgttca agcccgccgc
     1801 cgcgctaacc gccgccgcgc tcgtcgcggt caacccgtgg ttcgtctggt actcgcagga
     1861 agcgcgcgcg tacgcactgc tcaccctgat cgcgaccgcg tcactgctgt tcttcctgcg
     1921 cgcgctgaag gccgagtccc gccgcgacct gaccctctgg gcgctgttct cggcgctggc
     1981 ggtcctcacc cactacttcg cggtcttcct ggtcggggcg gaggccgtct ggctcctgtg
     2041 gacgatgcgc gagcgcgccg cccgcccggt cgccgcgctg gccgccgtgg gcctcgcgct
     2101 cgtaccgctc gcgatctcac agcggggcga ggggcacacg gcgttcatcg aggacatcgc
     2161 cctcggcagc cggatcgccg acctcccgaa gcgcttcgtc accggggagc ttggcacccc
     2221 gaccccgctg atcggcccgc tcgccgggct cctggtggtc gcgggcatcg cgctggcgct
     2281 gtgctggcgc ccggagcggg agcgagcgtt cgggctcctt gccctcgtaa cggccgccgt
     2341 tctggtgccg gtcctgctcg ccgtcgtcgg gccggacctc ctcctcccgc gcaacgcgat
     2401 cgcggcctac gtcccgttgg cgatcgtggt ggccgccggc ctcgcgacac accgcgcggg
     2461 cctggccgcg gcgggggtgc tcgccgcgct catgctcgcc gtcaccatcc agatctcgct
     2521 caacgagcac ctccagcgcg acgactggcg cgccgtggcc gccgcgctcc gcgacgaccc
     2581 gccgggggcc ggccgcgcca tcgtggtcac ctccgagccg cagatcgcgc cgctcgagca
     2641 ctacctcccc ggactcgccg agtacccgcc ccgcggcgcc gcgctcaacg agatcgtcac
     2701 gatcgaggtg acccgcgagg gcgcctcccg cgaaccggtg gtgccggcgg gcttcacgcc
     2761 ggccgagcgg cgccgcgagc cgagcttcga gctcgtacgc ctccagtcag gctcgatcca
     2821 ggtcccgccc gcgcaggtgc tcgcggacac gcgcctcgac gacgaggccg ccgccctgcg
     2881 cttccaaccc cgtgagtact ctcggacccc atgagcgccc cccgcatagc cgccctgccg
     2941 ggcgacggca tcggccccga gatcatggcc gccgcgcgcg agctgctggc cgcgctcggc
     3001 gagttcgaga tcgaggagca cctggtcggc ggcgcctcga tcgacgccca cggcaccgcg
     3061 ctgaccgacg ccgtcctcga cgcctgcagg caggcggacg ccgtcctgct cgccgccgtc
     3121 ggcggcccga agtgggacac caccgatccc gacaagccgc gtcccgagca gggtctgctc
     3181 ggcctgcgca agggcctcgg cctgttcgcg aacctgcgcc cggtccgccc gagccaggcg
     3241 ctgctcgact cgagcccgct gcgtcgcgag cggatcgagg gcaccgacct gctggtggtc
     3301 cgcgagctca ccggcgggct ctacttcggc gagcgcggcc gccgcgacgg caccgccttc
     3361 gacaccctcg tctacagcga ggaggaggtc gaccggatct gcgaggtcgc cttccgcttc
     3421 gcgcgcacga aggtcacgag cgtggacaag gccaacgtgc tcgagacgag ccggctctgg
     3481 cgcgccgtgg ccgagcgggt cgcggagcgg caccccgaca cgccgctcga ccacctgctc
     3541 gtcgacaacg ccgccatgca gctcgtcgcg cgcccggccg acttcgacgt gatcgtcacc
     3601 gagaacatgt tcggcgacat cctgagcgac gtcgccgcga tgctcaccgg ctcgatcggc
     3661 atgctcccga gcgcaagcgt gggcgccggc gggccgggcc tgttcgagcc cgtccacggc
     3721 tcggctccgg acatcgccgg cacgggcagc gccaacccgc tcgcgatgtt cggctcggtg
     3781 gcgatgatga tgcgctacgg actcggtcgc gaagacgacg cggcgcgcgt agaatcggcg
     3841 gtcgatcgcg cgctggagga agggcttcgc acccgggacc taggcggcga agccagcacc
     3901 gaggaggcca ccggggccgt cctctcgaat ctctgaacag gagacgccct gtgaacaccg
     3961 cggacctcat ctggatgaac ggcgagttcg tcgcttggga ggacgcgaag gtgcacgtac
     4021 tcacgcacgc gatgcactac ggcacgggcg tcttcgaggg gatccgctgc tacgagaccg
     4081 agcaggggcc ggcgatcttc cgccaccagg accacctcca gcggctgcgg cgctcggcgg
     4141 acctctacta catggacctg ccgtactcga cggaggagat ccgcgaggtc acgcacgagc
     4201 tggtcgcgcg caacgggctg cgctcctgct acatccgccc ggtcgtgcac cgcggctacg
     4261 ggccgatggg cctcgacccg ctcgacaacc cggtcgagtg cttcatcgcc gcctgggagt
     4321 ggggctccta cctgggcgac gagggcaaga ccaaggggat ccgcgcgaag acgagctcgt
     4381 ggcggcgcat atcgccggag tcgcagcccc cgcaggccaa ggcgtgcggt cagtacctca
     4441 acagcgtgct cgccaaggtc gagtcgcgca aggccgggtt cgacgaggcg atcctgctcg
     4501 accacaaggg cgtggtctgc gagggcacgg gcgagaacgt gttcgtcgtg cgcgacggcg
     4561 agatcgccac gccgccgatg agcgcgagca tcctcgacgg catcaaccgc cgctcgatca
     4621 tcgagatcgc ccgcgacctc ggctaccggg tgaccgagcg cgacatcgcg cgcgaggagc
     4681 tggcgctggc ggacgaggtc ttcctcaccg gcaccgccgc cgagctcacg ccgatgcgcg
     4741 agatcgacga catcgagatc ggcccccccg gcgagatcac gcttcagatc cagcgcgtct
     4801 tcgacgacgc cctgcacggc cgggccgagc gctaccgcga gtggctcgac ctcgtgcagg
     4861 taccgtcgaa ggcgtgaccc agcaggtcca gatctacgac accaccctgc gcgacggcat
     4921 gcagggggag ggcatgtccc tctcggtgga ggagaagctg cgggtggcgc acgccgtcga
     4981 cgagctcggc tggcacttca tcgaggcggg cttcctcagc tcgaacccga aggacgagga
     5041 gttcttccgc ctcctcgagc acgagcggtt cgcgaacgtc gaggtggccg ctttcgggat
     5101 gacccggcgg cgcggcgtgg ccgccgacga ggatccggcg ctgcggctgc tcgcggactc
     5161 gttcgcgccc gtgtgcacgc tcgtcggcaa gacctggaag ctgcacctgg agaaggtcat
     5221 caaggtcgat cccgacgaga acctcgcgat gatcgccgac tcggtggggt tcctccgtga
     5281 gcacggcaag cgcgtggtgt acgactgcga gcacttcttc gacgcctacc gcgaggaccc
     5341 cgagtacgcc acgcgctgcg tccgtgcggc cgccgaggcc ggcgcggagt gggtcgcgct
     5401 ctgcgacacc aacggcgcaa ctctgccgga tgcgatcgcg tcgaacacac acacggtcgc
     5461 gatggagttc gcgtcggcct gcggaatcgc catccacacg cacgacgatg ccggctgcgg
     5521 cgtggcgaac tcgctcctcg cggtcgacgc gggcgcccgc caggtgcagg gcaccgtcaa
     5581 cgggtacggc gagcgctgcg gcaacgcgaa cctcaccacg atcgtgcccg acctccagct
     5641 caagctgggc tacgagtgcg tgtcggccga tcagctggcc ggtctcaccg aggcggccca
     5701 cctgatcgac gagatctgca acgtgagccc gaacccgaac cagccctacg tgggccgcaa
     5761 cgcgttcgcg cacaagggcg ggatgcacgt ggcgggcgtg ctgtcggacg cgcgcacgtt
     5821 cgagcacgtc gatccggccg ccgtgggctc cgatcgcgcg ctgctcatct ccgagctctc
     5881 cggcaagggg accgtgcagg cgcgcgccga gcaggcgggc ctcggcatct cggcggacga
     5941 ggctgtgcgc gtggtggacc gcgtgaagga actcgagtcg cgcggcttcc acttcgaggc
     6001 ggccgacggc tcgttcgacc tcctgatccg gcgcgagacc ggcgactacg agccgctgtt
     6061 ccggctcgag tcgtggcgcg tgatcgcgga gaagcgcgag gacggccggg tgcagaccga
     6121 ggccacgatc aagatctggg tcggcggtga gcgctacctg cgcacctgcg agggcaacgg
     6181 cccggtcaac gccctcgacc gcgcgctgcg cgccgcgatc ggcgacgtct acccgcacct
     6241 gcgcgacatc gagctcgtca actacaaggt ccgcatcctc gacgaggaca agggcacggg
     6301 cgcggtcacg cgcgtgctcc tcgacgccag cgacggaacc gacacctggg gcacgatcgg
     6361 cgtctcggag aacatcatcg aggcgagctg ggaggctctc gtggactcgc tcgaggccgg
     6421 gatgctgccg cgtcgcagcg ggcacacgcg ccgggctgcg ggcgagccgg cgacgccttg
     6481 atccccctcg cacggccggt catcggccag cgggaggagg agctcgtcct cgaggtcctg
     6541 cgctccggcc acctgtcgct ggggccgatg ctgccgcgct tcgagcacgc cttcgccgac
     6601 cggctcgggg tcggcttcgc cagcgcggtg tccagcggca cggccgggct ccatctggcc
     6661 gtgcgggccg ccggcgtcga gccgggcgac gaggtggtca cgtcgccgtt cagcttcgtc
     6721 gcgagcgcca actgcgtggt ctacgagggc gcgcggccgg tcttctgcga catcgaccgg
     6781 cgcaccctca acatcgaccc ggccgcggcc gcggcggcgg tcacagagcg gaccgctggc
     6841 ctcctgccgg ttcacatctt cggctacccg gcagacatgc ccgcgctcga gcggctggcg
     6901 gtggatcgcg ggctgtggat cgtcgaggac gcctgcgagg cgctcggcgc cacccacggc
     6961 gacgggtgcg tcgtgggcgc acgcggcaac ctcgcggtgt tcgcgttcta tgccaacaag
     7021 cagatgacca ccggtgaggg cggggtggtg atctgcccga tcgaggcgca caaggcgcgg
     7081 ctcgactccg agcgcaacca gggccgcgcg cccgacatgg gctggctcga ccacgaccgg
     7141 ctcgggttca actaccggat gagcgacctc cagtgcgcgc tcggcgtcgc tcagctcgag
     7201 cggctcgacg gcatgctggc gctgcgcgcg gccgtcgcgg cgcactaccg cgaggcactg
     7261 gccgggatcg aggggctcga gctgccgtgc ccggacgtag gcggcgacat gcgcggctgg
     7321 gtcgtgtacg tggtgcagct cccgccgggg gtggaccgcg acgcgaccat cacggcgctt
     7381 cgtgagcggg ggatcgactc gaagccgtac ctgccggcga tccacctgat gaccttctac
     7441 cgcgagcgct tcggccaccg cgagggcgag ttcccggtct gcgaggacgt ggccgcgcgc
     7501 tcgctcgcgc tcccgttctt ccccggcctc gccgagtccg aggtggagcg aatcggcgaa
     7561 aatttgcgcg ccgtcctgta ttcctagtgg taatgcgcca ggcgctgatt gccgtgatgg
     7621 tcctcctgct ggtcccggcg ggcgcctcgg ccgcgaccgc cagcgtggac gtgcacgtga
     7681 gcaccggcaa ggggtgcagc gtgtcgccgg agagctgtgt gcaggcggag ctggacttca
     7741 cggccgcggc cggtgaggcg aacgacgtcg aggtctcgac cgcgggcagc tcgctggtcg
     7801 tgcgggaccc cggcaccggg gtaagcgccg gcagcggctg ccagcagcag ggcccgaaga
     7861 tggtccgctg ctcgccgccc gaggggccgt cgatgggctt cgtcctgatc agcgtggtgc
     7921 tcggcgacgg ggtcgacgtg ctccggaaca ccacgggcat cgtctcgtcg gccaccgggg
     7981 gatcgggcga cgatcgtctg accggcggta cgtcgtcgga cacgttcgac ggcggcgccg
     8041 ggcgcgacag gctcgaaggt gcggacggct ccgactacct ccaggacacc ggcccggtcg
     8101 agcccgacgt gctcgacggc ggcgccgggt acgacaaggt cgactactcg atgcgcgcgg
     8161 cacgcgtgac ggtcgacctg cgcggccccg cgagcccgtc cggtgcggcg ggtgaggacg
     8221 actcgctggt cggcatcgag gagggccacg gcgggcgcgg gccggacctc ttgcgcgccg
     8281 gcgcggatcc ggtcgtgctg gtcggtcacg gcggggcgga ccggatcatc ggcgggttcg
     8341 gcaacgacct gctgatcggc aacaccggcg acgacaagct ctatggcatc ggtggcagcg
     8401 accgcctcgt cggcggggcc ggaaacgacg gcctccgtgc gggctgcggc cgctcgaagc
     8461 tcttcggcaa cgccggcaac gaccgattcg acagccgcaa cggcgcggcg aaccgcgtgg
     8521 tgggcggagc cggccgcgac gtggcggtgc tcgaccagct cgacacgacg cagcaggtgg
     8581 agcgccgcac ccgccagcac atcgacgcct gcgcgctgta gcgccccgtc gccccgccgc
     8641 cccgccgccc cgccgccccg ccgccccgcc gcc
//
LOCUS       DASVPL010000209        18797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_103993, whole genome shotgun sequence.
ACCESSION   DASVPL010000209 DASVPL010000000
VERSION     DASVPL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18797
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103993"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VF517_10970"
     CDS             complement(<1..531)
                     /locus_tag="VF517_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX8053504.1"
                     /translation="MAKGGTHDERSLIEHQSEPNDATPFLPEKRTLGALREAAAGCRG
                     CHLWRGATQTVFGEGRKRARVVLVGEQPGDREDRAGQPFVGPAGRELDRGLEAAGIDR
                     GEAYVTNVVKHFKFEERGRRRIHATPKRFEIDACKPWLEEELKLLSPEAVVLLGATAG
                     KAILGASFRVTQQRGRP"
     gene            610..1659
                     /locus_tag="VF517_10975"
     CDS             610..1659
                     /locus_tag="VF517_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8053505.1"
                     /translation="MGTEDRIEQLIDSAEARGSSCVNLSELSELVAELELDDDQTQAA
                     HERIEARGLDVSDDCGKDCEPTKIVNGEMVTATTDALQLFLNEIRRHPLLSKDEEIEL
                     AQAIERGDLEAKERMINSNLRLVVSIAKKYQGNELPLLDLIQEGILGLIRASEKFDWR
                     KGYKFSTYATFWIRESIQRGLANRGRTIRLPVHIGQRERKIARTQRELSVKLGREPTD
                     EEVAEAAELTLDDMREVRDAARTVTSLDRPVGEDEDTGLGDLLPSGDLAPEEEVEISL
                     RESALRKAIEQLPDTEREVVRLRYGLNGAGEPTPLREAGRRLGMSAEGVRKLESKALA
                     RLAMARELEGLRSVA"
     gene            complement(1660..2823)
                     /locus_tag="VF517_10980"
     CDS             complement(1660..2823)
                     /locus_tag="VF517_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053506.1"
                     /translation="MSDLRVVREFRDELTGAPPPRRRPATRRAAAAAVALVVLGVGIA
                     TAATGEFPPVGDPLPAPRHDNARRPLSPVTGTGRVAPIRTADPEGGPPWSVRTFRTRG
                     GAECEQVGRVVKGRLGVIGDDGEFHEAPLASVACFSRRRRAAPADATLLRTHGSPNQG
                     PGIPATGTPPREYAPVPCEDGVSAGQSFDPHTVVCGDRPRRFIDTGTVGPNVVAIEVR
                     GGGVQERHPVVAGRYMIVREAVQPPRSVAYAVFRDGRRVRVWKAPRKRTTPVQRDAGV
                     IRRTGLHASPAHVGRDTLVTVSMRARFYPKSRYGGWFDIDLTGPRGCPRQGRVRFTVF
                     LDRMARAGERLRFGVRPPGLTHTERADWCSGRYTGRVLFRSRIDLGTFSFERP"
     gene            complement(2820..3329)
                     /locus_tag="VF517_10985"
     CDS             complement(2820..3329)
                     /locus_tag="VF517_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8053507.1"
                     /translation="MEFEPIYDACAERLLVYFTRRTLDPETALDLWAETLAQGYAGWR
                     RFRGTTQDEGVAWLYAIAANQWAGYLRRGYAERRAMRRLGIERPELARDDIERLEELA
                     GLTELRGQVGAALGALPAEQQEALRLRVVEELPYAEVAERLNVTEPAARARVSRGLRR
                     LGLTLEEAT"
     gene            complement(3373..5061)
                     /locus_tag="VF517_10990"
     CDS             complement(3373..5061)
                     /locus_tag="VF517_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053508.1"
                     /translation="MAVTVLATLAPTQAQAITGGAVDARFGSCGQATVLIPRAVAGRQ
                     FSYGAGSSLAVQPDGKVIAAGPAARGMGATRFNADGSIDTTFGDGGVAFIAADVPRSD
                     ETQVTAVALQPDGKVLAAGWLRTQGPGDDPGLSLVERFVIARFTAAGQPDPSFGGDGI
                     VIEAPAGSSSASIHGIAPLPGGGLVVAGQVDDRFAVGRYRDDGTLDPGFGEAGVARVT
                     TGTRQRGRGHAVAVQPDGRVLAAGDTNADGNSRTWTLARLTASGAPDASFGGTGTVTE
                     TLDESSTASTLVPLADGRFYAVGTTVDFWGNDEGGGTTRRAATIRYLPNGARDTAFAG
                     DGSVLDALGQGLYSAVSPTGAALDANGRLTLATQYGPLVRYTEAGARDLAFGTEGILR
                     VFSAPSGMSIAARPDGSLFLGGVNSRQGSRPTGFEWGAAIMRLAGSGQALESLKGQPA
                     ACAFRVRNTSIRHLLRRSRTARYGRVLVGAFLPQPTPGGGQVVARATAGGRTFELGSH
                     SFATNYAGSSSFEVPIRKASYARLARARSVRIELTLTLADRDTTAATAARTLSR"
     gene            complement(5235..6386)
                     /locus_tag="VF517_10995"
     CDS             complement(5235..6386)
                     /locus_tag="VF517_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8053509.1"
                     /translation="MSCRLPALVLSLIAVQAAPAQAATVDVFDGNLRFVAAYGESNHP
                     TFHYRAEDELVVFDSSLARRAVVAGPGCAQDGESTVICPRAGVTGASFFLGGRPRGED
                     LPDSLTLTADVPVPVTAAASSGANARVSYIDLRPIAASLDGMANDGPAGRGDNLGRGI
                     GAIDGGDGTDSLVGNNLANALDGDNGADSLSAGGGDDTVTAASYNDVGADAVGLETRG
                     ADTIDCGPGDDLVYYDVSDKQVNCELLVLVRDFGYVYGGTPGPDRIIADRGPAFVHGN
                     LGNDRLGVTRFAGGTSLFGDEGNDRLAGNTGSDRIEGGLGNDVLVGAEGDDRLFGNRG
                     LDRIAAGPGADTISARDGQADTISCGSGRDRVTADRRDRIGRDCERVSR"
     gene            6491..7345
                     /locus_tag="VF517_11000"
     CDS             6491..7345
                     /locus_tag="VF517_11000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX8053510.1"
                     /translation="MKVGVLTFPTEYSVQPDELGRMVEEHGFESLFVAEHTHIPVSRE
                     SPYPAGGELPREYSHTYDPFVALTAAAAVTTELKLATGICLVVQRDPITTAKEVATLD
                     RICGGRFMFGVGAGWNREEMADHGTDPGTRFSLMRERVEAMKAIWTQEEAEYHGRHVS
                     FDPLWQWPKPLQEPHPPVLLGGNGPKVLDRVVSFADGWMPNWGLQTNEELIARVEELQ
                     RLASEAGRGRIPVTVYAARAKLEVLGPLAEAGVERAVLWIPPAPRDEVAPRLERLASL
                     ARDLNGEP"
     gene            complement(7342..8712)
                     /locus_tag="VF517_11005"
     CDS             complement(7342..8712)
                     /locus_tag="VF517_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8053511.1"
                     /translation="MTTAQLNEVSRLRDRVGRVVAPADPAWDESRQAYNLAVDQHPAL
                     IAYPQDAQGVAEVIRFARERDLRVAPQRTGHNARPLGALEHTILLKTDDLTGVEIDRL
                     AGRARVGSGMKWEHVVPQASDMGLAALHGSTPDVSIAGYSLGGGLGWYGRKYGLAANS
                     VTAIEIVTSDGDLRRVDHQHEPELFWALRGGGGNFGVVTALEFDLYPVREVYAGVMFF
                     PVERASEVLHAWRELTPTLPDEMTTVGRILQFPPLEEVPEPMRGQSFVLVEGFYLGGQ
                     AAGEELLRPVRELGPAMDTFAMVPPVGLSEMHMDPPDPVPAVSDSMLLGDLTAEAIDG
                     MVAVTGPGSGSMLVTVEFRHMGGALGRTQPHHGAIAKLDGEFAMFAAGMAMEPGMAAA
                     NEAQLALLTNALAPYESGRYGNFVETKADTSSFFDADTYSRLQRVKGEYDPDGLFRAN
                     HVIEAA"
     gene            9287..11965
                     /locus_tag="VF517_11010"
     CDS             9287..11965
                     /locus_tag="VF517_11010"
                     /inference="COORDINATES: protein motif:HMM:NF027421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COR domain-containing protein"
                     /protein_id="HEX8053512.1"
                     /translation="MEEARTTGVFRIHEESLGRWPEELRHISGLRELVMTDCRLSAIP
                     RWIADLTDLEFLDLANNPIIRLPTSIGTLQSLRSLGLASVPIRELPASLSGLESLEKL
                     VLSGTRLKQLPSWIGHLSALQMLDAGRLELSTLPNSVRQLQKLTHFYLWNHGFETPPP
                     ELHNLTSLEVLDLSHSGSMSLEPHRQQASMADAPSRLVGASASGSGPLRALPEWLAND
                     LPALQWLYLGGQRLERLPPRLPPALRGLYAASNFIERVDPTLFDLSALAELDLRANAI
                     TDLDPPVGAISRLRYIALADNPLPIPPEVLSGSEPGRIIPYLSQVQGPAKPLDQAKVI
                     VVGEGSVGKTSLVRRLVANTYSPLEQKTEGIAVTRWGVDAGGAPITLDIWDFGGQEIM
                     HATHQFFLTKRSLYMLVIDARQGDEQNRLEYWLKLIQSFSAGSPVILVGNKTEQSPLD
                     IDTRGLMAKYPNVRSILAVSCSTGEGVGSVREVLTDAIAEMPHVRDLLPVAFFDIKER
                     LERLDENYLSFGAYQELCRRHGLTNLEQQELLVGFLHDLGTVLCFREDPRLSDTNILN
                     PDWVTGGVYRLLNSHLAAQRKGLLTWDDVDSILDTIDYPRERRSFIVDMMKRFELCYE
                     ADDTFLVPDLLTKEEPDTGSWADALHFEIKYDVLPSSLISRLIVRMHASISHATVWRT
                     GLVVSLDENRGLVKGDREDAVIRIWVTGNPSGRRGLLTAVRRELRAIEGTIPGLIGEE
                     RVPVPRHPGVWVPYSHLLDLESAGRDTVVPQGLTMDFSVQELLAGIEAPSERADPAMR
                     SLPESDPASIIRKGDGAWVPSDAIRLGMLLIVVMVVAAAVFVGAERIVGTNAAAGIAA
                     VALAAVALVAVVVLRSSGQLSEAGFLDAVRQTLRRE"
     gene            complement(12031..14838)
                     /locus_tag="VF517_11015"
     CDS             complement(12031..14838)
                     /locus_tag="VF517_11015"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8053513.1"
                     /translation="MAPAGEVQLLERGRELESLDSLIDGAAAGDGRIALIEGQAGIGK
                     SRLLAEAREHAQSAGMRVLTAKGSELELEFAFGVVRQLFEPSLVDAETRAAWLTGAAA
                     AAGPVFDAVGDAAEDAPRDGSFAALHGLYWLVVNAAADGPVMLALDDLHWVDRPSLRF
                     VAYLARRLEGLPVLIAATLRSAEPGSDPALLGEIANDPSTASVRPGALSEEGVCRLVR
                     ARLADDADSAFCSACHDTTGGNPLLLRQLLSSLESEGVAPESRNATVVRDIGPRAVSR
                     TVLLRLARLSADAAAVARAVAVLGENADVPAAAALAEIDDRVAAEATADLARAEILRP
                     DPPLAFVHGLVRDAIYHDLPPGERELQHGRAARMLADGGASLEQVASHLLTVPRRGDE
                     WVYDQLVQAARSALRKGAPESAVSYFTRALDEPPPADRRAGLLMELGMAEVSTFGPDA
                     ATHLRMAHDELEGPDRAMAAYALARTLLFTGAAGEGAALAERTMDEVPEEALELRQAL
                     ESLIYYGIVFGGAPQAGLQRYDRYRSGAPGDGPGAKMLEAVAAYDWAVSGGNSEEVAE
                     LAIRALEGGLLVREDNGLSNIPPIASLALADRDEALEYLDEAEADAHRRGAMFAALGV
                     NCWRSFTLWCRGELPDADAEGRMGVEKMELWGQQTGQVMAYSMSILGRIATERGDLDT
                     AREMLGDAGELVPEAHSTFLWATARAELALAEGKPEEAIEVCDQACRSLHRIVNPAWI
                     PWRTLKARALDRLGRTEEALELALEELPPSRAWGAPGTLARTLREIGTIEREDGIERL
                     RESVSVAEGSHAKLEHAKCLFALGSAVRRSRQPTDAREPLRRALELAFVCGAPVLAEE
                     IRTELHATGVRPRTEALSGPAALTAKEAKVASLAAEGQTNRDIAQSLYVTPKTVEVHL
                     TNAYRKLGIRSRRELAGALAA"
     gene            complement(14839..15192)
                     /locus_tag="VF517_11020"
     CDS             complement(14839..15192)
                     /locus_tag="VF517_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8053514.1"
                     /translation="MAPFTSDPGHEVAPERVKEMLDRGEIVLIDVREQYEWDAGRIAG
                     AKHIELERLAARADEIPRDRPVVFQCRLGARSAMATEAFRASGYEAYNLTGGISAWVD
                     HGLPLDPDGGVVAEH"
     gene            complement(15271..15738)
                     /locus_tag="VF517_11025"
     CDS             complement(15271..15738)
                     /locus_tag="VF517_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053515.1"
                     /translation="MAPPLLPIAVLALGSAGQAGPPAVALAPPVAGLYRGATAQEQRI
                     ALRVGPGGRRASWRIAYRARCDDGARIRGAYRSGDGTPLAAVGPGGTFRVTGEEPARF
                     RGGPTGAARFSLTGQIGPDGGSGTWRVEVFPPRPGAARVSCTSGPVRWGVARG"
     gene            complement(15887..17917)
                     /locus_tag="VF517_11030"
     CDS             complement(15887..17917)
                     /locus_tag="VF517_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8053516.1"
                     /translation="MLLWTALVAIVAMLPASPAAAAETESWIVVYEHGVDSVDRATDA
                     RERRRGFRSRLRFRRAVRGFSARLTAEQVRELRADPAVESVVPDRPVRASAAQPLAPG
                     EPIAPSGVRRLGLAAPSSSSAGWVRQASDVGVAVIDTGIDLDHPDLTAVDGVDCIAPG
                     TTAEDENGHGTHVAGTIGAENDGAGVTGVAPGTRLHAVRVLDAAGSGSTSSVVCGIDW
                     VLANASARGIRVINMSLGGAGETDTDCGVTSGDPFHAAVCRATSAGILNVVAAGNEAA
                     DVRAVPVDATDPTQGTGPGSPAVYPEVLTVSAINDHDGAPGAQGTLDPNECGVDDRRA
                     PYSNFATLAADRAHMIAAPGTCITSTWLDGTYATIDGTSMATPHVAGAVALCIGEAGE
                     PGPCAGLTPAQIVERLRTEARAYRLANPGSGFAGDPEQPFPTGTTRYYGYLLHPLLAG
                     PGSSFASTPPDTTTDPTPAFEFASQAPGATFECSLDGGAFQACGRNHETAPLADGSHQ
                     LAVRAVDVAGNRDATPAVDSFTVDAVQDPQPVVEPGPPVTTTTTTPPAEPDRTAPAAS
                     LGVATRQRIGTVLRKGIRVSMLCSESCRADASVIIASSEAAKLGLGRRAVTGGRKGLE
                     LGGGKRTVAIKLTSAVRKRLALARSVLVQLRVTVTDAGGNARTVKKSVRLTR"
     gene            18030..>18797
                     /locus_tag="VF517_11035"
     CDS             18030..>18797
                     /locus_tag="VF517_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8053517.1"
                     /translation="MGERTEYKPGTFSWAELATTDPDGAKGFYGGLFGWEAQDMPAGE
                     SGVYTMCSLRGSYVGAVMEQGEQEREMGIPPHWNNYVTVDDVDASTARASELGATVAA
                     GPFDVMEAGRMSAIQDPTGAFLCLWQPRDHIGASLVNEPGCLTWNDLNTPDPDTAREF
                     YEALFGWTYEKMDSDEADYWITWNGERTNGGLMRMETEGAPSFWIPYFAVEDVEAATE
                     KATSSGGGKHKGPITVPVGRFSILHDPAGAVFAVYEGK"
BASE COUNT         2675 a         6674 c         6760 g         2688 t
ORIGIN      
        1 cggccgcccg cgctgctggg tcacgcggaa gctcgcgccc aggatcgcct tgccggcggt
       61 ggcgccgagc agcaccaccg cctccggcga gagcagcttc agctcctcct ccagccacgg
      121 cttgcaggcg tcgatctcga accgcttcgg cgtcgcgtgg atccggcggc ggccgcgctc
      181 ctcgaacttg aagtgcttga ccacgttggt cacgtaggcc tcgccgcgat cgatccccgc
      241 cgcctcgagc ccgcggtcga gctcgcgccc cgcggggccc acgaacggct gcccggcgcg
      301 gtcctcgcgg tcgcccggct gctcgcctac cagcaccacc cgcgcgcgct tgcgcccctc
      361 gccgaacacg gtctgggtcg cgccgcgcca gaggtggcag ccgcggcagc cggcggccgc
      421 ctcgcgcagg gcgccgaggg tgcgcttctc gggcaggaac ggcgtcgcat cgttcggctc
      481 cgactggtgt tcgatcaagg aacgctcgtc gtgcgtacca cccttggcca cggcgcgagg
      541 atacgcccaa gtgagatttg ggctacctaa cctgggtact agagagccat ggaggcagga
      601 ggaccaaacg tgggtacgga agaccggatc gagcagctga tcgactccgc cgaggcgcgc
      661 ggctcgagct gcgtcaacct ctccgagctg agtgagctcg tcgccgagct cgagctcgac
      721 gacgaccaga cacaggcggc gcacgagcgg atcgaggcgc gcggcctcga cgtcagcgac
      781 gactgcggca aggactgcga gcccacgaag atcgtcaacg gcgagatggt cacggccacg
      841 accgacgcgc tccagctctt cctcaacgag atccgccgcc atccgctgct ctcgaaggac
      901 gaggagatcg agctggcgca ggcgatcgag cgcggcgacc tcgaggccaa ggagcgcatg
      961 atcaactcga acctgcggct cgtggtctcg atcgcgaaga agtaccaggg caacgagctg
     1021 ccgctgctcg acctgatcca ggagggcatc ctcggcctga tccgcgcgtc ggagaagttc
     1081 gactggcgca agggctacaa gttctcgacg tacgcgacct tctggatccg cgagtcgatc
     1141 cagcgcgggc tggccaaccg cgggcgcacg atccgcctgc cggtccacat cggccagcgc
     1201 gagcgcaaga tcgcgcgcac gcagcgcgag ctgtcggtga agctcgggcg cgagccgacc
     1261 gacgaggagg tcgcggaggc cgccgagctc acgctcgacg acatgcgcga ggtgcgcgac
     1321 gcggcgcgca cggtcacgag cctcgaccgg cccgtcggcg aggacgagga caccggtctc
     1381 ggcgacctgc tcccgagcgg cgacctcgcc cccgaggagg aggtcgagat cagcctccgc
     1441 gagagcgctc tgcgcaaggc gatcgagcag ctaccggaca ccgagcgcga ggtggtgcgg
     1501 ctgcgctacg gcctcaacgg cgccggcgag cccacgccgc tccgcgaggc cggccgccgg
     1561 ctcggcatgt cggccgaggg cgttcgcaag ctcgagtcga aggccctcgc gcggctcgcg
     1621 atggcgcgcg agctcgaggg cctgcgctcg gtcgcctagc tacgggcgct cgaagctgaa
     1681 cgtcccgagg tcgatccggc ttcggaacag cacgcggccg gtgtagcgac cgctgcacca
     1741 gtcggcgcgc tcggtgtgcg tgagtccggg gggtcgcaca ccgaaccgca gccgctcgcc
     1801 cgcacgggcc atgcggtcga ggaacaccgt gaagcgcacg cggccctggc gcgggcagcc
     1861 acgtggaccg gtcaggtcga tgtcgaacca cccgccgtac ctgctcttcg ggtagaagcg
     1921 ggcgcgcatc gagaccgtga cgagcgtgtc gcggccaacg tgcgcgggcg aggcgtgcag
     1981 gcccgtgcgg cggatgacgc cggcgtcgcg ctgcaccggc gtggtgcgct tgcgcggcgc
     2041 cttccagacg cggacccgcc ggccgtcgcg gaacacggcg tacgcgaccg agcgcggcgg
     2101 ctggaccgcc tcgcggacga tcatgtaacg cccggccacg acgggatggc gctcctgcac
     2161 accaccccca cggacctcaa tcgcgaccac gttcggcccg acggtgccgg tatcgatgaa
     2221 gcgacgcggg cgatcgccgc acacgacggt gtgcgggtcg aaggactgcc cggcgctcac
     2281 gccgtcctcg cacggcaccg gggcgtactc gcgcggtggt gtgccggtgg cggggatgcc
     2341 cgggccctgg ttcgggctgc cgtgcgttcg cagcagcgtt gcatcggccg gggccgcgcg
     2401 gcgccggcgg ctgaagcagg ccacggacgc cagcggcgcc tcgtggaact cgccgtcgtc
     2461 gccgatcacg ccgaggcgcc ccttcaccac ccgcccgacc tgctcacact cggcgcctcc
     2521 gcgcgtgcgg aaggtgcgca cgctccacgg cggcccgccc tccggatccg cggttcggat
     2581 cggcgccacc cggccggtgc cggtgaccgg cgagagggga cggcgcgcgt tgtcgtgcct
     2641 gggagcgggg agcggatcgc ccacgggcgg aaactcgccg gtggccgcgg tggcgatccc
     2701 caccccgagt accacgagtg ccacggcggc agcggccgcc cggcgcgtag cgggccggcg
     2761 ccgcggcggc ggcgctccgg tcagctcgtc gcggaactcg cgcaccacgc gcaggtcgct
     2821 catgtcgcct cctccagcgt cagtccaagc cgccgcaggc cccgcgagac ccgcgcacgc
     2881 gcggccggct cggtcacgtt caggcgctcg gccacctcgg cgtacggcag ctcctcgacg
     2941 acgcgcaggc gcagcgcctc ctgctgctcg gccggcaggg ctcccagtgc cgcgccgacc
     3001 tggccgcgca actcggtcag gcccgccagc tcctcgagcc gctcgatgtc gtcgcgcgct
     3061 agctcggggc gctcgattcc gagccgccgc atcgccctgc gctccgcgta gccgcggcgc
     3121 aggtagccgg cccactggtt cgccgcgatc gcgtagagcc aggccacgcc ctcgtcctgc
     3181 gtggtgcctc gaaagcgccg ccagccggcg tagccctgcg cgagggtctc ggcccacagg
     3241 tcgagcgccg tctccgggtc gagggtgcgc cgggtgaagt agacgagcag ccgctcggcg
     3301 caggcgtcgt agatcggctc gaactccatc gtcaccgaca gagttgcgcg agccgcccct
     3361 ggtgtgacgc gcctagcggc tcagcgtgcg cgcggccgtg gcagcggtgg tgtcgcggtc
     3421 ggcgagcgtg agcgtcagct cgatgcggac gctgcgcgcg cgggcgaggc gggcgtacga
     3481 ggccttgcgg atcggcacct cgaaggacga gctgccggcg tagttggtcg cgaacgagtg
     3541 cgagccgagc tcgaaggtac gcccgccggc cgtcgcgcgc gcgacgacct gccccccgcc
     3601 cggcgtcggc tggggcagga acgcgcccac cagcaccctc ccgtagcgcg cggtgcggct
     3661 gcggcggagg aggtggcgga tgctcgtgtt gcgcacgcgg aaggcgcagg cggcaggctg
     3721 gcccttcagg gattcgagcg cctgcccgga gccggccaga cgcatgatcg ccgcccccca
     3781 ctcgaacccg gtcggacggc tgccctggcg ggagttaacg cccccgagga acagcgagcc
     3841 gtcgggccgc gcggcgatgg acatgcccga gggcgcggag aagacgcgga ggattccctc
     3901 ggtgccgaac gcgaggtcgc gcgcgccggc ctccgtgtag cgcacgagcg ggccgtattg
     3961 ggttgcgagc gtcagccgac cgttcgcgtc gagcgcggcg ccggtcggcg agaccgccga
     4021 gtagaggccc tgaccgaggg cgtcgagcac ggagccgtcg ccggcgaagg cggtgtcgcg
     4081 cgcgccgttc gggaggtagc ggatggtcgc cgcgcgccgg gtcgtgccac cgccctcgtc
     4141 gttgccccag aagtcgacgg tggtcccgac cgcgtagaag cggccgtccg cgagcggcac
     4201 cagcgtggag gcggtggagc tctcgtcgag ggtctccgtg accgtgccgg tgccgccgaa
     4261 gctcgcgtcg ggcgcgccgg aggcggtcag gcgtgcgagc gtccaggtgc gcgagttgcc
     4321 gtccgcgttg gtgtcgccgg ctgcgagcac gcggccgtcc ggctgcacgg cgaccgcgtg
     4381 gccgcgcccg cgctggcgcg tgccggtggt gacccgggcc acgccggcct cgccgaagcc
     4441 ggggtcgagc gtgccgtcgt cgcggtagcg ccccacggcg aagcggtcgt cgacctgtcc
     4501 cgccacgacc agcccgccgc cgggcagcgg cgcgatgccg tgtatggagg ccgagctcga
     4561 gccggcgggt gcctcgatca cgatcccgtc gccgccgaag ctggggtccg gctggccggc
     4621 ggccgtgaag cgggcgatca cgaagcgctc cacgagcgag aggccggggt cgtccccggg
     4681 cccctgcgtg cgcagccagc ccgcggcgag caccttcccg tcgggctgga gcgccacggc
     4741 ggtgacctgg gtctcatcgg agcggggcac gtcggcggcg atgaaggcca cgccgccgtc
     4801 gccgaaggtc gtgtcgatgc tcccgtcggc gttgaagcgg gtggcgccca tgccgcgcgc
     4861 agcggggccg gcggcgatca ccttgccgtc cggctggacg gcgagcgacg agccggcgcc
     4921 gtagctgaac tggcggcccg ccaccgcgcg cgggatgagt accgtggcct gcccgcagct
     4981 cccgaagcgg gcgtcgacgg cgccgccggt tattgcctgg gcttgggtgg gggcgagggt
     5041 tgccaggacg gtgacggcca cggcaacgac aacggcagca tttaggggaa gggttcggga
     5101 cgggtgccgg ctccgccgga ggcccgggcc gcgcgcggct ccgcccgttt gccggaggcg
     5161 cgccccgccg cgcgcggctc cgcccgcccg cggccggaag ccccggccgc cgcgcgcggc
     5221 tccgcccgcc cgcttcaccg gctcacccgc tcgcagtcgc ggccgatccg gtcgcggcgg
     5281 tcggccgtca cgcggtcgcg gccgctgccg caggagatcg tgtcggcctg accgtcgcgg
     5341 gccgagatcg tgtcggcacc ggggcctgcc gcgatgcggt cgaggccgcg gttgccgaac
     5401 aggcggtcgt cgccctccgc cccgaccagc acgtcgttgc cgagcccgcc ctcgatccgg
     5461 tcgcttccgg tgttgcccgc cagccggtcg ttgccctcgt cgccgaagag cgacgtgccg
     5521 cccgcgaatc gcgtgacccc gagacggtcg ttgccgaggt tgccgtggac gaaggcgggg
     5581 ccgcggtcgg cgatgatccg gtcggggccc ggagtgccgc cgtagacgta gccgaaatcg
     5641 cgcacgagca cgagcagctc gcaattgacc tgcttgtcgc tgacgtcgta gtacacgagg
     5701 tcgtcgccgg ggccgcagtc gatcgtgtcc gcgccgcggg tctccaggcc gaccgcgtcc
     5761 gcgcccacgt cgttgtagga cgccgccgtg accgtgtcgt cgcccccacc ggcggagagg
     5821 ctgtccgctc cgttgtcgcc gtcgagcgcg ttggccaggt tgttgcccac gagcgagtcg
     5881 gtgccgtcgc cgccgtcgat cgcgccgatc cccctgccga ggttgtcgcc gcgccccgcc
     5941 ggcccgtcgt tggccatgcc gtcgaggctc gccgcgatcg gccgcaggtc gatgtacgag
     6001 acgcgcgcgt tcgcaccgct ggacgccgcc gcggtcacgg gaaccggcac gtccgcggtg
     6061 agcgtgagcg agtcgggcag gtcctcgccg cgcggcctcc cgccgaggaa gaacgaggcg
     6121 ccggtcacac cggcgcgcgg gcagatcacc gtcgactcgc cgtcctgcgc gcagcccggg
     6181 ccggcgacca cggctcggcg ggcgagactc gagtcgaaga cgaccagctc gtcctcggct
     6241 cggtaatgga aggtcgggtg gttggactcg ccgtaggccg ccacgaaccg caggttgccg
     6301 tcgaacacgt ccacggtcgc ggcctgcgcg ggcgccgcct gaacggcgat cagtgagagc
     6361 acgagggctg ggaggcggca gctcatccgc gcaatgaaac cgcttttgcc gacgcgagtt
     6421 tcgctcggct ttcctgactt atgggtccta tacgcccaaa agtcaggaaa gctgcgacag
     6481 aatgccgctc gtgaaggtcg gcgtgctcac ctttcccacc gagtactcgg tccagccgga
     6541 cgagctgggg cggatggtcg aggagcacgg cttcgagtcc ctgttcgtgg ccgagcacac
     6601 ccacatcccg gtgagcaggg agtcgcccta ccccgccggc ggcgagctgc cgcgcgagta
     6661 cagccacacc tacgacccgt tcgtggcgct caccgcggcc gccgcggtca cgaccgagct
     6721 caagctggcg accggcatct gccttgtggt ccagcgcgac ccgatcacca ccgccaagga
     6781 ggtcgccacg ctcgaccgga tctgcggtgg gcgcttcatg ttcggcgtgg gggccggctg
     6841 gaaccgcgag gagatggccg accacgggac cgaccccggc acgcgcttct cgctgatgcg
     6901 cgagcgggtc gaggcgatga aggcgatctg gacccaggag gaggccgagt accacggccg
     6961 ccacgtctcc ttcgatccgc tctggcagtg gccgaagccg ctccaggagc cgcatccgcc
     7021 ggtgctgctc ggcggcaacg gcccgaaggt gctcgatcgc gtggtgtcgt ttgccgacgg
     7081 ctggatgccc aactgggggc tccagacgaa cgaggagctg atcgcgcggg tggaggagct
     7141 ccagcggctg gcgagcgagg cgggccgcgg gcggatcccc gtgacggtct acgccgcccg
     7201 cgccaagctc gaggtgctcg gaccgctggc cgaggccggc gtggagcggg cggtgctctg
     7261 gataccgccc gcgccccggg acgaggtcgc gccgcgcctg gagcgcctcg cgagcctcgc
     7321 ccgggacctg aacggggagc cctaggccgc ctcgatcacg tggttggccc ggaacagtcc
     7381 gtccgggtcg tactcgccct tcacgcgctg gagccggctg taggtgtcgg cgtcgaagaa
     7441 ggagctcgtg tccgccttcg tctcgacgaa gttgccgtac cggcccgact cgtacggcgc
     7501 cagcgcgttc gtcaggagcg cgagctgcgc ctcgttggcc gctgccatcc ccggctccat
     7561 ggccatgccg gcggcgaaca tcgcgaactc gccgtcgagc ttcgcgatag cgccgtggtg
     7621 tggctgcgtg cggccgagcg cgccgcccat gtggcggaac tcgaccgtca cgagcatcga
     7681 ccccgacccg ggcccggtaa ccgcgaccat gccgtcgatc gcctcggccg tcagatcgcc
     7741 caggagcatc gagtcgctca cggccgggac gggatccggc gggtccatgt gcatctccga
     7801 caggcccacg gggggcacca tcgcgaacgt gtccatggcc ggcccgagct cgcgcaccgg
     7861 gcgcagcagc tcctcgccgg cggcctggcc accgaggtag aagccctcca ccagcacgaa
     7921 cgactgcccg cgcatcggct ccggcacctc ctcgagcggc gggaactgga ggatccggcc
     7981 gaccgtggtc atctcgtcgg gcagggtcgg cgtgagctcg cgccaggcgt gcagcacctc
     8041 gctcgcgcgc tcgaccggga agaacatcac gcccgcgtag acctcgcgca cggggtagag
     8101 gtcgaactcc agcgcggtga ccacgccgaa gttgccgccg ccgccgcgca gcgcccagaa
     8161 cagctcgggc tcgtgctggt ggtcgacgcg ccgcaggtcg ccgtcggagg tgacgatctc
     8221 gatcgcggtg acgctgttcg ccgccaggcc gtacttgcgc ccgtaccagc cgaggccgcc
     8281 gccgagcgag tagccggcga tcgagacgtc gggggtcgag ccgtgcagcg ccgcgaggcc
     8341 catgtcggac gcctgcggca cgacgtgctc ccacttcatg ccggagccga cgcgcgcacg
     8401 gccggcgagg cggtcgatct cgacgccggt caggtcgtcg gtcttcagca ggatcgtgtg
     8461 ctcgagcgcg ccgagcggcc gggcgttgtg gccggtgcgc tgcggggcga cgcgcaggtc
     8521 gcgctcgcga gcgaaccgga tcacctcggc cacgccctgc gcgtcctgcg ggtaggcgat
     8581 cagcgcgggg tgctggtcga cggccaggtt gtaggcctga cgggactcgt cccaagccgg
     8641 atcggcgggg gcgacgacgc ggccgacgcg gtcgcggagc cggcttacct cgttgagctg
     8701 tgcggtggtc atggtgttgc tcctgttctg ggggcgctga caaagatgga tgcgcggagc
     8761 gccgcgatca gcccgaacag cccgcggggg agggaccgct cgacaccggc ggggaaggtc
     8821 cccgtcccgg tgaggcggac gccggcgagg cgagtctcgg ggctgatcgc ggtggtcgtt
     8881 ccgtgcatgc ttcgcagctt cggccaccgc cagaccgcgc acatcgagga caaccccaag
     8941 gttcgacccc agtctttcgg gcctggcggt cttagggggg gttggggtac tggcggcacc
     9001 gcaccgccgg ccccaagcag cgcgcacacc accacgagat ccgcccctcc aggggccccg
     9061 cgagagcgcc ttaagaccta gatcacgtcg tccgcacggg atggatcgga agcttgtagt
     9121 cccgcgcggg tcccgcattt aggaatatcg cgatgaagct cgtttcggcc atcatctccc
     9181 tcgcccgccg atccgttcca gagcgcattc gaatgtaagg tagtgagcag ccatcgtcag
     9241 gtcgacctag cgcgtcttgc ccactccccc cacacttacc gcccgcttgg aagaggcacg
     9301 caccacaggc gtgtttcgta tccacgaaga atcgcttggg aggtggcctg aggagttgcg
     9361 ccacatcagc ggcctgcgcg agctcgtgat gaccgattgc agactgagcg cgattccgcg
     9421 ctggatcgca gacctcaccg acctcgaatt tctcgatcta gcgaacaatc cgatcatccg
     9481 cctcccgacc tcgatcggca cccttcaaag cttacgatca cttggccttg ccagtgtccc
     9541 cattcgggag ctaccggctt ccttgtcggg gctcgagtcg ctcgagaagc tagtcctgtc
     9601 cgggacacgc ctcaaacagc tccccagttg gatcggccac ttgagcgcct tgcagatgtt
     9661 ggacgcaggc cgccttgagc tatcgactct gccgaactcg gtcaggcagt tacagaagct
     9721 aacgcacttc tacctgtgga accacgggtt cgagacgccg ccgcccgagt tgcataacct
     9781 gacctcgctc gaggttcttg atctcagtca cagtgggtcc atgagcctag agccacaccg
     9841 ccagcaagcc tcaatggctg atgcaccgtc tcggctagtt ggcgcgagcg cgagcgggag
     9901 cgggccgctc cgggcgctac cggagtggct cgctaacgac ctaccggcac tgcagtggct
     9961 gtacctcggt ggacagcgct tggagcgcct accaccccgg ctgccgccag ccctacgcgg
    10021 cctgtacgcg gcgagcaatt ttattgaacg cgtggaccca acactcttcg acctaagcgc
    10081 tctggccgag ctggatcttc gcgcgaatgc aattaccgac ttggatccgc ccgtgggtgc
    10141 aattagccgg ctccgctaca tcgccctcgc agataaccct ttgccgatcc cgcctgaagt
    10201 gctatctggc tcggagccgg ggcggatcat tccgtattta tcgcaggtcc aaggcccagc
    10261 caagccgctt gaccaagcaa aggtaatcgt cgtcggcgag ggatcggtgg gcaagacatc
    10321 gcttgttagg cgtctcgtgg ctaacacgta ctccccactc gagcagaaga ccgaaggaat
    10381 tgcagtcacg cgctggggcg tcgacgcagg cggagcgccg ataacacttg atatctggga
    10441 cttcggcggg caagagatca tgcacgctac acaccagttt tttctaacta agcgcagcct
    10501 ctacatgtta gttatcgacg ctcgccaggg agatgagcag aaccgactgg aatattggct
    10561 taaattgata cagagctttt cggctgggtc gccggtgatc ctcgtcggca ataaaaccga
    10621 acagtcgcca ctagacattg acacgcgcgg tctgatggcg aaatatccca acgtgaggtc
    10681 aatcttggca gtttcgtgta gcacgggcga gggcgttggg tctgtgcgcg aagtattgac
    10741 agatgcgatc gccgaaatgc cgcatgttcg tgatctccta cccgtcgcgt ttttcgatat
    10801 caaggaacga ctcgagcgac tcgacgagaa ctatttatct tttggcgcgt accaagaact
    10861 ttgtcgccgg cacggcctaa caaacttgga gcagcaagag ttgctcgtgg ggttcttgca
    10921 cgatctcggc accgtgcttt gcttccgtga agaccctcgg ctatccgata caaatatctt
    10981 gaacccagac tgggtcacgg gaggagtcta ccgactgctc aactcgcatc tcgccgcaca
    11041 acgtaagggt cttctgactt gggacgatgt tgattcgatc ctcgatacca tcgattaccc
    11101 acgtgagcga cgctcgttca ttgtcgacat gatgaaacgc ttcgagctct gctatgaggc
    11161 agacgatacg tttcttgttc ccgatttact caccaaagag gagccggata ctggatcgtg
    11221 ggctgacgcg cttcacttcg agattaagta cgacgtgctt ccatcgagct tgatctctcg
    11281 tctgattgtg cgtatgcatg cgtcgattag tcacgccacg gtctggcgta ccggtttggt
    11341 ggtgtccttg gacgagaatc gtgggcttgt gaaaggcgac cgtgaagacg ctgtgatccg
    11401 catatgggtc acaggcaatc cgagtggcag gcgcgggctg ctcacagcag tacgacgtga
    11461 gctgcgtgcg atcgaaggaa ctatcccggg gcttatcgga gaggagaggg tgccggtccc
    11521 tcgtcaccct ggtgtctggg tgccttacag ccatctgctg gaccttgaaa gcgcgggacg
    11581 cgacaccgtg gttccgcagg ggctcacgat ggatttcagc gttcaggagc tgctggctgg
    11641 gattgaagcc ccaagcgaac gcgctgatcc agcgatgagg tcactgcccg aatctgaccc
    11701 agctagcatc atccgtaagg gtgatggtgc ctgggtgccg agcgacgcga tccggctggg
    11761 gatgttgctc atcgtcgtaa tggtggtcgc cgcagccgtc ttcgtaggcg cggagcgcat
    11821 cgtgggcacc aacgctgcgg ccgggatagc ggccgttgcg ctcgccgctg ttgcgctcgt
    11881 agcggtcgtg gtacttcgat cgtctggcca gctcagcgag gccggcttcc tcgatgccgt
    11941 gcggcagact cttcgccgag aatgaccggg tccccgcgct cgattccgcg gtgattgctc
    12001 ggccccgcca gcctcactta agcgggtgcg tcaggccgcc agcgcccccg ccagctcgcg
    12061 gcgggagcgg atgccgagct tgcggtacgc gttcgtcagg tggacctcga ccgttttcgg
    12121 cgtgacgtag aggctctggg cgatgtcgcg gttggtctgg ccctcggccg ccagcgaggc
    12181 caccttggcc tccttggcgg tgagggccgc gggaccgctg agcgcctcgg ttcgcgggcg
    12241 cacgccggtg gcgtgcagct ccgtgcggat ctcctcggcc aggaccgggg cgccgcagac
    12301 gaaggccagc tcgagcgcgc ggcgcagcgg ctcgcgcgcg tcggtcggct ggcgcgagcg
    12361 gcgcaccgcg ctgcccagcg cgaacaggca cttcgcgtgc tcgagcttgg cgtgcgaacc
    12421 ctccgccacg gagaccgact cgcgcagccg ctcgatcccg tcctcgcgtt cgatcgtgcc
    12481 gatctcgcgc agcgtccgcg ccagcgtccc gggggcgccc cacgcgcggc tcggaggcag
    12541 ctcctccagc gccagctcga gggcctcctc ggtgcggccg agccggtcga gcgcgcgtgc
    12601 cttgagggtc cgccacggga tccaggcagg gttcacgatg cggtgcagcg atcggcaggc
    12661 ctggtcgcac acctcgatcg cctcctcggg cttgccctcg gcgagcgcca gctcggcgcg
    12721 agccgtggcc cagaggaagg tggaatgcgc ctcggggacg agctcgccgg cgtcgccgag
    12781 catctcccgc gcggtgtcga ggtcgccgcg ctcggtcgcg atccgcccga ggatcgacat
    12841 cgagtaggcc atgacctgac cggtctgctg gccccagagc tccatcttct cgacccccat
    12901 gcggccctcg gcgtcggcgt cgggcagctc gccccggcac cacagggtga acgagcgcca
    12961 gcagttgacc ccgagcgccg cgaacatcgc cccgcggcgg tgggcgtcgg cctccgcctc
    13021 gtccaggtac tcgagcgcct cgtcgcggtc ggccagcgcc agcgaggcga tcggcgggat
    13081 gttcgagagg ccgttgtcct cgcgcacgag caggccgccc tcgagcgccc tgatcgccag
    13141 ctcggcaacc tcctcggagt tgccccccga cacggcccag tcgtacgccg cgaccgcctc
    13201 gagcatcttc gcgcccggcc cgtcgccggg ggcgccgctg cggtagcggt cgtagcgctg
    13261 gagaccggcc tgcggggccc ctccgaacac gattccgtag tagatgaggc tctcgagcgc
    13321 ctgcctcagc tccagcgcct cctcggggac ctcgtccatc gtgcgctcgg cgagcgccgc
    13381 tccctccccc gccgcgcccg tgaacagcag cgtgcgggcg agcgcgtacg cggccatcgc
    13441 gcggtcgggg ccctcgagct cgtcgtgggc catgcgcagg tgggtggccg cgtccggacc
    13501 gaaggtggag acctcggcca tgcccagctc catcagcagt ccggcgcgcc ggtccgcggg
    13561 cggcggctcg tccagcgcgc gggtgaagta cgagacggcg ctctccggcg cgcccttgcg
    13621 cagcgcggag cgcgcggcct ggacgagctg gtcgtagacc cactcgtccc cgcgacgcgg
    13681 caccgtgagc aggtgcgagg ccacctgctc cagcgaggcg ccgccgtcgg ccagcatccg
    13741 cgccgcgcgg ccgtgctgga gctcgcgctc gcccggcggg aggtcgtggt agatcgcgtc
    13801 gcggacgagc ccgtgcacga atgccagcgg cgggtccggc cgcaggatct cggcacgcgc
    13861 gaggtctgcg gtggcctccg cggccacgcg gtcgtcgatc tcggccaggg cggcggccgc
    13921 cggcacgtcc gcgttttcgc cgagcaccgc cacggcgcgg gccacggcgg cggcgtcggc
    13981 cgacaggcgc gcgagccgga gcagcaccgt gcgcgagacc gcccgcggcc cgatgtcgcg
    14041 caccaccgtc gcgttgcgcg actccggcgc cacgccctcc gactcgagcg agctcaggag
    14101 ctggcggagc agcagggggt tgccgccggt ggtgtcgtgg caggcgctgc agaaggcgct
    14161 gtcggcgtcg tcggcgagcc gcgcgcgcac cagccggcac acgccctcct cgctcagggc
    14221 gcccggacgc accgacgcgg tggaggggtc gttcgcgatc tcgcccagca gcgcgggatc
    14281 ggagcccggc tcggcgctgc gcagcgtggc ggcgatcagc accggcaggc cctcgagtcg
    14341 ccgcgcgagg taggccacga accgcagcga cggccggtcc acccagtgca ggtcgtcgag
    14401 cgcgagcatc accggcccgt ccgcggccgc gttcaccacc agccagtaga gcccgtgcag
    14461 ggcggcgaac gagccgtcgc gcggtgcgtc ctcggccgcg tcgccgacgg cgtcgaagac
    14521 cgggccggcg gcagcggcgg cgccggtcag ccaggcggcg cgcgtctcgg cgtccaccag
    14581 cgagggctcg aagagctggc gcaccacgcc gaacgcgaac tccagctcga gctcgctccc
    14641 cttcgccgtg aggacgcgca ttccggcgga ctgcgcgtgc tcgcgcgcct cggccagcag
    14701 cctgctcttg ccgatcccgg cctgaccctc gatcagcgcg atgcggccgt cgccggcggc
    14761 ggcgccgtcg atcaggctgt cgagggactc gagctcacgc ccccgctcga ggagctggac
    14821 ttcgccggcg ggggccactc agtgctcggc caccacgccg ccgtcgggat cgagcgggag
    14881 accgtggtcc acccacgcgg agatcccgcc ggtgaggttg tacgcctcgt agcccgacgc
    14941 gcggaacgcc tccgtggcca tcgcggagcg ggcgccgagg cggcactgga agaccaccgg
    15001 ccggtcgcgc gggatctcgt ccgcacgggc ggccaggcgc tcgagctcga tgtgcttggc
    15061 ccccgcgatc cgccccgcgt cccactcgta ctgctcgcgg acgtcgatca gcacgatctc
    15121 gccgcggtcg agcatctcct tgacgcgctc gggcgcaacc tcgtgcccgg ggtccgaggt
    15181 gaagggggcc atgccctcaa tctacgcgct acccgagtga aatcgcaacg ggcccggcgg
    15241 gtgcgctgcg cccgccgggc ggggccgggg tcagccgcgc gccacgcccc agcggaccgg
    15301 tccgctcgtg cagctcacgc gggcggcgcc cgggcgcggt gggaagacct cgacgcgcca
    15361 tgtgcccgag ccgccgtcgg gaccgatctg gccggtcagg ctgaagcggg cggcgccggt
    15421 gggaccgccc cggaagcgag ccggctcctc gcccgtcacg cggaaggtcc cgccagggcc
    15481 gacggccgcg agcggcgtgc cgtcgccgga gcggtacgcg ccgcggatgc gcgctccgtc
    15541 gtcgcagcgg gcgcggtacg cgatccgcca gctcgcgcgc cggccgcccg ggcccacgcg
    15601 gagcgcgatg cgctgctcct gggcggtggc ccctctgtag aggcccgcga cgggcggtgc
    15661 gagcgccacg gccggaggcc cggcctgccc ggcggagccg agagcgagga ccgcgatggg
    15721 gagcagcgga ggcgccatgt ccgcgtcgcg acgctagcgg ggaggccgga gggaccggcc
    15781 gtcgtcatgc acttccgttc gacattcctg ccctgagggt ccatatgacc cataagtcag
    15841 gaatgtcgcg cggtgcccgg gcctctcgga cttccgtgga gcgccgctaa cgcgtcaggc
    15901 gcacggactt cttcacggtg cgggcgttgc cgcccgcgtc cgtgaccgtc acgcgcagct
    15961 gcaccaggac gctgcgcgcc agcgccagcc gcttgcggac cgcgctcgtg agcttgatcg
    16021 ccaccgttcg cttgccgccg ccgagctcca gccccttgcg cccgccggtg accgcgcgcc
    16081 tgcccagtcc cagcttcgcc gcctcgctgc tcgcgatgat cacgctcgcg tccgcgcggc
    16141 agctctccga gcagagcatg ctcacccgga tccccttgcg cagcaccgtg ccgatccgct
    16201 ggcgggtggc gaccccgagg gaggccgccg gcgcggtccg gtccggctcc gccggcggcg
    16261 tggtcgtcgt cgtcgtgacg ggcgggccgg gctcgaccac cggctgcggg tcctgcaccg
    16321 cgtccaccgt gaacgagtcc actgcggggg tggcgtcccg gttgccggcg acgtcgaccg
    16381 ctcgcaccgc gagctgatgc gagccgtccg cgagcggcgc ggtctcgtga ttgcggccgc
    16441 atgcctggaa ggcgccgccg tcgaggctgc actcgaaggt cgcgcccggg gcctgcgagg
    16501 cgaattcgaa cgccggggtg gggtcggtgg tggtgtccgg cggtgtcgag gcgaacgagc
    16561 tgcccgggcc ggcgagcagc ggatgcagga ggtagccgta gtagcgcgtg gtgccggtcg
    16621 ggaagggctg ctcgggatcg cccgcgaagc cgctgcccgg gttggcgagc cggtaggcgc
    16681 gcgcctcggt gcgcagccgc tcgacgatct gggcgggcgt gagacccgca cacgggcccg
    16741 gctcgcccgc ctcgccgatg cagagcgcca ccgccccggc cacgtgcggc gtggccatgc
    16801 tcgtgccgtc gatcgtggcg tacgtgccgt ccagccaggt ggaggtgatg caggtgcccg
    16861 gcgcggcaat catgtgcgcc ctgtcggccg ccagggtcgc gaagttcgag tacggggcgc
    16921 gtcgatcgtc gacgccgcac tcgttcgggt cgagcgtccc ctgggcgccc ggagcgccgt
    16981 cgtggtcgtt gatggcgctc acggtgagca cctccgggta gaccgccggg ctgccggggc
    17041 cggtgccctg ggtcggatcg gtcgcatcga ccgggaccgc ccgcacgtcc gccgcctcgt
    17101 tccccgcggc gaccacgttg aggatccccg ccgacgtggc gcgacacacg gcggcgtgaa
    17161 acggatcgcc gctcgtcacg ccgcagtcgg tgtcggtctc gcccgcgccg ccgaggctca
    17221 tgttgatcac ccggatgccg cgcgcggagg cgttcgccag cacccagtcg atcccgcaca
    17281 ccacgctgct cgtgctgccg ctcccggcgg cgtccaggac gcgcaccgcg tgcaagcgcg
    17341 tgccgggcgc cacgccggtc acgccggcac cgtcgttctc ggccccgatc gtgcccgcca
    17401 cgtgcgtccc gtggccgttc tcgtcctcgg cggtcgtgcc gggcgcgatg cagtccaccc
    17461 cgtccaccgc ggtcaggtcc ggatggtcga ggtcgatgcc cgtgtcgatc acggccacgc
    17521 ccacgtcgct cgcctggcgc acccagcccg cggagctcga cgacggtgcg gccagtccga
    17581 ggcggcgcac cccgctcgga gcgatcggct cgcccggcgc gagcggctgc gccgcggagg
    17641 cgcgcaccgg ccggtcgggc acgacggact cgacggccgg gtcggctcgc agctcgcgaa
    17701 cctgctccgc cgtgagccgc gccgagaagc cccgcaccgc acgccggaag cgcagccggc
    17761 tgcggaaccc gcgccggcgc tcgcgcgcgt cggtcgcgcg gtccacgctg tcgaccccgt
    17821 gctcgtagac cacgatccag ctctcggtct cggcggccgc cgcggggctc gccggcagca
    17881 tcgccacaat ggccacgagg gcggtccaga gcagcacgcg cgaggccttt acttcaaaaa
    17941 gtgaagcgat acctggatgg taggccgacc acgtacgcgc gtcagtggca ggatgccgat
    18001 ctgaacacga cgacgaaggg acttccgcag tgggtgagcg aaccgagtac aagccgggca
    18061 ccttctcctg ggccgagttg gcgacgaccg accccgacgg cgccaagggc ttctacggcg
    18121 gcctgttcgg ctgggaggcg caggacatgc cggccggcga gagcggcgtg tacacgatgt
    18181 gcagcctgag gggatcgtac gtcggcgcgg tgatggagca gggcgagcag gagcgcgaga
    18241 tggggatccc gccgcactgg aacaactacg tgacggtgga cgacgtcgac gcgtccacgg
    18301 cgcgcgcgag cgagctcggc gccaccgtcg ccgccgggcc gttcgacgtg atggaggcgg
    18361 gccggatgtc ggcgatccag gaccccaccg gcgcgttcct gtgcctatgg cagccgcgcg
    18421 accacatcgg cgcgtcgctc gtgaacgagc ccggctgcct cacctggaac gacctcaaca
    18481 cccccgaccc cgataccgcc cgcgagttct acgaggccct gttcggctgg acctacgaga
    18541 agatggacag cgacgaggcc gactactgga tcacatggaa cggcgagcgc acaaacggcg
    18601 ggctgatgag gatggagacc gagggagcgc cgtcgttctg gattccctac ttcgccgtcg
    18661 aggacgtcga agccgccact gagaaggcga cctccagcgg cggcggcaag cacaaggggc
    18721 cgatcaccgt cccggtgggc cggttctcga tcctgcacga tccggccggc gcggtgttcg
    18781 ccgtctacga gggcaag
//
LOCUS       DASVPL010000210        39852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_24704, whole genome shotgun sequence.
ACCESSION   DASVPL010000210 DASVPL010000000
VERSION     DASVPL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 39852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39852
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24704"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VF517_11040"
     CDS             <1..945
                     /locus_tag="VF517_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053518.1"
                     /translation="RPAMPTLRPQELSGLVVIPTVEHDPGIHRVSIAIDVGVSRTGAG
                     IAPPEVVTREDLVVVLRNASEGSLEAIASPDPGALPVRAIRVVQARGEFTFSQGVNPP
                     DELVVTLRGDQKTFPMSQTLSPTRCLGKEPQVGDRAPGLPRKGSFLHRLPFIQRLRRS
                     RCCVKRLDAPVNPSADATAKSEHFEVEADFVARGAKCQCGCCEYRQYVRGTFSDAAGA
                     PVRFDLPSGALDPASYCEDGAIDEFGPGAHGYYGHRSTSSPGDAYAGAGKSASCTYRG
                     NERPSCPPTDGVHLEFLGLVIDVCQGRVAAKRTWIVDL"
     gene            complement(976..1785)
                     /locus_tag="VF517_11045"
     CDS             complement(976..1785)
                     /locus_tag="VF517_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX8053519.1"
                     /translation="MSHWILLVAPLALASFVMLFAFVGCQLETGGLGGPEQYKDSVTN
                     NTNLVGYWRLGEAVGATTAVDSKGGHNGTYVGGVTLGQPGFVVGDSDTAAQFDGSTGH
                     VTVPHSDALNPAKFTVEAIADVAGGEGHRAVASSRDISPDTQIFGYAIYVNNENKWEA
                     WVGDGASGVGVAAGGPDATPGRHYLAMTYDGKTLKLYVDPAADAPATAEVVYQPNTAR
                     DFRIGAGGNESDTPQHLFNGVVDEVAVYNVDLDFATIQSHFELATNGPSSG"
     gene            complement(1764..2450)
                     /locus_tag="VF517_11050"
     CDS             complement(1764..2450)
                     /locus_tag="VF517_11050"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX8053520.1"
                     /translation="MRRVLFSWHGVNVPSYPAMLYVGLLCGVYAGYGVATSMPIDPDR
                     ASLALLILIAPALVGSRLWFVLSHWEVFRREPRRIWRRGDGGAALVGGLLLAVAVSPA
                     VLAGLGLPFAAFWDVATFTMLVGMVFTRVGCLLNGCCAGRPSDGRVALHLPDHAGRWE
                     RRHPVQLLELAAAVTLLAGAAAIATAPPFPGAIFVFALAGYGCVRSILNPLRETHGTR
                     KELECRTGSF"
     gene            complement(2453..2881)
                     /locus_tag="VF517_11055"
     CDS             complement(2453..2881)
                     /locus_tag="VF517_11055"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX8053521.1"
                     /translation="MASWVLAGAGAVVLLDQVTKSLAVRFLASAGVGGARRSGVFRLA
                     ANGGSWLGRAPSLPAQLGLWSMTLACTVLVLATGAGSPSHGLAGAGLVVALGGATSNL
                     LDRAGRGAVIDFIALGWWPAFNLADVAIVAGVAVAVGSVV"
     gene            complement(2900..3532)
                     /locus_tag="VF517_11060"
     CDS             complement(2900..3532)
                     /locus_tag="VF517_11060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8053522.1"
                     /translation="MTGSRVPASGVAVRPARADDIAAAGLVFDAAATAYTRLAGSADD
                     GRRIVARVWSQPGHSASFEHALVAEIDGQAVGVLIGFPVRDRYRLHRALLLTSLRDLR
                     PGRWPLLAAALPQIVLASPRPPRGAYYIGTIAVARHARWRGVAGTLAHHAEIAAEARG
                     FRHMVAHTGTRHAIARRALENHGFRLARARRNGYALYVKGIGATIDAGAS"
     gene            3644..4438
                     /locus_tag="VF517_11065"
     CDS             3644..4438
                     /locus_tag="VF517_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053523.1"
                     /translation="MRRPLAALALAAAGLLAVPAVAQVPGDPVRVEPSTAGTSSHMIV
                     DIHGSADPKANGRPATHAVIDLAQGFRRDLRARSERCTESRAKAFDCPGKSKIGSGTA
                     DATVSNGAISQAVVADVSLFLAPRAQSSDAAGVWLIFTERSSGSRAWIFGRLLKLGSH
                     GPFGIEARFENFETANSAAPEGFTVRLDRIQVEVGASRTEKITVCCKTVRRNGKKKKV
                     RYRKKVVRNLLHNPKSCDGSWDYQVRIRYSATDESVRDGVMPCTNP"
     gene            complement(4586..4677)
                     /locus_tag="VF517_11070"
     tRNA            complement(4586..4677)
                     /locus_tag="VF517_11070"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4641..4643),aa:Ser,seq:gct)
     gene            complement(4714..5277)
                     /locus_tag="VF517_11075"
     CDS             complement(4714..5277)
                     /locus_tag="VF517_11075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8053524.1"
                     /translation="MRSFAGFAVIAMLAAALYGGAAVATGASGDGGNGDAAAVIGASQ
                     IGLQYEPAAPRRARASGLIWPVRGAITGRFGEQRGGHRHAGIDIPIADGTPIRAALDG
                     VVVMREWQDGYGNYTCLAHAGGFVTCYAHQSRFRVKQGAKVSQGEVIGFVGDTGTSDT
                     AHLHFEVRSGRAPWGEPQDPLKRLPRG"
     regulatory      complement(5285..5440)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            5502..6128
                     /locus_tag="VF517_11080"
     CDS             5502..6128
                     /locus_tag="VF517_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053525.1"
                     /translation="MDRRLLYALIALATVLIALVATQVLVPSYLERRAENRLTEDGGK
                     ATVDIDALPALGLLFDHGKRIQVRGEELVIPLEGERGKVFGDLDRFAEADVRLDRMVA
                     GPLRIARFSLARGKHGDPYALTVSATVTAAELGDYAGQQLGPLAGFLGRIGASVLPFS
                     NAPIPVELDASLKSDDGRAVVETVDGTVAGLPADPLVGALAEAIAARL"
     gene            6146..6352
                     /locus_tag="VF517_11085"
     CDS             6146..6352
                     /locus_tag="VF517_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053526.1"
                     /translation="MDEEDPTTQELRVEQLQRERIEREAAAAGATPDDTEQHERRADR
                     ASYLREKLEERAEAERDAAREDDD"
     gene            6366..6629
                     /locus_tag="VF517_11090"
     CDS             6366..6629
                     /locus_tag="VF517_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053527.1"
                     /translation="MEYDERADELEKEAERLEHESKRLGERTDAARSEWESAKSDPSK
                     APGAQAAEATGPHHIDAEDPATGRRYGEVRQEEIEDAEAADSE"
     gene            6720..7694
                     /locus_tag="VF517_11095"
     CDS             6720..7694
                     /locus_tag="VF517_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX8053528.1"
                     /translation="MLGRHQRNEDREPDRPRGGTLGQAIAAARDQLGLEIVCVSRTVR
                     ASDLSDHAGDACGVDPLADGSAIVPLESGDGHLHGSLVCRPVDGHELDDRDLGFLRVL
                     GRVVGTQIAHSERDRKLRRQQAETTGLQALLAAIEARDSYTGEHSRSVVDLSVRVARE
                     LQLGGRDLDQVEQVALLHDVGKVAVPDAVLQKNGPLTEPEVELVRRHPVVGARIVSSV
                     SELAHLAPAIRSGHERWDGRGYPDGLSGDEIPILSRITFVCDAYDAMVSDRPYRDALP
                     PQVAITEVERNAGSQFCPRASAALLGVLETTRSHARSATRFFTAPPVP"
     gene            7719..8867
                     /locus_tag="VF517_11100"
     CDS             7719..8867
                     /locus_tag="VF517_11100"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEX8053529.1"
                     /translation="MQTNELNSDLLRRLADLRPEGARVLSLFLNLDPSEFATPPARAT
                     EARSLIDQAERQLRESGELDHDAEKALRQDIERARELFDRLDADGAHGLAVYVCGPAD
                     LFEAIKLPRPIETRAVVDDSPFVEPLAELGMRGSWCVMVVNRQVARMLRGSPDQLEEL
                     PVVEDEVHGRHDQGGWSQARYQRSVDEEVRWHLKHAAEVAFRRFKRSPFDNVLLGGPE
                     ETVTEFEGILHPYLRERVAGHFKVDVENTTPDQIRAAAAPLIDAVDEKREREALDRLE
                     EGVGTGGRGTAGLDLTIAALNERRVEILLLSDRFDAAGVYCRGCGWVGTDVTLTACPV
                     DGGTLEERENIAENAIELAILQSAQVLRVRHHPERLEAHGSIGAVLRF"
     gene            complement(8864..10456)
                     /locus_tag="VF517_11105"
     CDS             complement(8864..10456)
                     /locus_tag="VF517_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8053530.1"
                     /translation="MADAVVIGAGQNGLVAANVLADAGWDVLVLEEQDAPGGAVKSGE
                     LTGQPGFVHDLFSAFYPLTVVSPAMRAMRLEEHGLVWRRSPLAFAHPAIDGTCPYVSL
                     DLEETVERLNHYENGDGEAWRRLYGIWERVGDPIMEAGFGAPFPPLRGAAKMAWALNR
                     DTVRFLRMITLSVRRFGEEWFVSEQARRMITGNALHADLTPETAVSAGFGWMLSVLGQ
                     HVGFPTPEGGAGQIIAALLKRLDARGGVVRCGQRVEAVIVRGGRAVGVRTADGTEIDA
                     PRAVIADVVAPKLFLDLVGEEHLPNRTVADLRRFEYDASTFKVDWALDGPIPWAAPDA
                     RRSGVIHTGEDMDHFTVHASELARKLMPSHPLMVVGQYAPVDSTRAPEGCDVAWAYTH
                     HPRTPKGDAGGQDITGRFDERETELFARRLEDEIEARAPGFRDLIRARHVFTPHDLER
                     ANSNLVNGAINGGTAQAHQQLVFRPTSGVGRPETPIRNLYLGSSSAHPGGAVHGACGA
                     NAARAALRWRGLLPGVSTLPPR"
     gene            complement(10592..10762)
                     /locus_tag="VF517_11110"
     CDS             complement(10592..10762)
                     /locus_tag="VF517_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053531.1"
                     /translation="MIGIVLAVLFAALVYAICVALGLPAIVGIVAAILVLLAGIPTGG
                     YGLGGRFGSRTH"
     gene            10929..11795
                     /locus_tag="VF517_11115"
     CDS             10929..11795
                     /locus_tag="VF517_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX8053532.1"
                     /translation="MPRSIWTGAISFGLVNVPVKLYSAVSKKTVRFHQLHEPDGVRIQ
                     QKRFCPADGEEVPYDEIVKGYEISPDRYVVISPEELDSLEPRKTKTVDIEDFVDLDEI
                     DPLYYDHPYYLLPGQGAQKPYKLLVEAMKQANKVAIARVVIRTKEQLVAIRPVGDILS
                     MASMNFADEVIDPDSFDEAPGDDVETRKREVEMAQQLIESLTSEFDPSKYHDTYRERV
                     MELIEAKADGQEIAVQPEEEVAPVPDLMAALEASLAAAKKDKGGSGNGKPKSAEPKKK
                     PAASKKKAAAKK"
     gene            complement(11865..12074)
                     /locus_tag="VF517_11120"
     CDS             complement(11865..12074)
                     /locus_tag="VF517_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053533.1"
                     /translation="MTDHQEGQDKQDEGGSPHTSPEEGHPAGTGERTDRDIGGPTAPE
                     DGGDGDETGGGPSPKPADEGFDSHQ"
     gene            12063..12839
                     /locus_tag="VF517_11125"
     CDS             12063..12839
                     /locus_tag="VF517_11125"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8053534.1"
                     /translation="MVGHSATPTRSPGLRLIRDLLARPERHSYGGSRSQRADLYVPDG
                     AGPHPVVVLLHGGYWRSKYGKSLMRAVSADLARRGFAAWNVEYRRMGRHGGGWPMTFD
                     DVGAAIDLLPEIGRGRLRLDAVDVVGHSAGGQLAIWAATRRDGRVPVRRVVAQSAVCD
                     MVWAGAAARELLGGGPDEVPERYVATNPMQLVPIGKPTLLVHGSDDETVPLERSRLYY
                     EAARAAGDPVELIEPSPGHHRIHIDPRSRAWQVVAEWLAG"
     gene            12850..13545
                     /locus_tag="VF517_11130"
     CDS             12850..13545
                     /locus_tag="VF517_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053535.1"
                     /translation="MTRVPEPVARATASVFRTLSSLRGKRAFHPHGLVFEAEFEPYLR
                     SSPTGVEILDGGPRRALVRCSRALGFPEPAPDILGFAIRFTDAYGAGRHQDLLCVTSI
                     DAPVGHHALIPATGFFTRPYSTLLAYRLPEGVRLFGVLPIGSGPALGPRLEQIAEAAR
                     RGTATFELALAPLSGRFDGVGTIQLGDQLPPEQGELLRFNPWNSGGGMCPTGPFMGLR
                     DAAYKGSQEGAAT"
     gene            complement(13546..13839)
                     /locus_tag="VF517_11135"
     CDS             complement(13546..13839)
                     /locus_tag="VF517_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053536.1"
                     /translation="MLTLEGIDGESIVLDGNWIEKLRIGNSRARNPISSYKETQVKDF
                     TRRKKVFFGEKEELIQITVVCQNFMSLIVPAERRADVEGFVAALDEAKAAAAE"
     gene            13860..14810
                     /locus_tag="VF517_11140"
     CDS             13860..14810
                     /locus_tag="VF517_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053537.1"
                     /translation="MTSLPLAHETWLTHDDVAVDWDFVLEGLTLALLAAAVLLTLAVR
                     LVARRRPGVDVPVLARMAPFMPFAVRLHLAVSLIGLVSLGYYLSPAMDLQADLAGILL
                     GAVMVIVAVAMVIGFQVRAAAWLLIAAGPLGMLEFGVSPVVQRIDMLGPALFLLFAGA
                     GRWSAEFELGRVRDPSPEAIARAVWALRVAAGVALIVVAFVEKLAQPDLALEFLHHHP
                     QFNVAREVGIPMGDLEFARVAGAIEVLFGLLLISGALPQAIVLIAGIPFNATLWFFGN
                     LELVGHLPVYGTMLVLLVYGSSPELRPLASRMWPWRPLRA"
     gene            14842..15297
                     /locus_tag="VF517_11145"
     CDS             14842..15297
                     /locus_tag="VF517_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053538.1"
                     /translation="MGSVWGIVGAPLALAKGVVSFTRDVGTIARVAREAEPVIREVEP
                     IVRDNSTTAREALDVARENNALTRQANATIATLDLHATELLRRVGTIELELPAIRRGL
                     AHVEDLADSAETLSVAAPPVQAAAERVERLAQRIPGARRSTRRLNGRDA"
     gene            15309..15698
                     /locus_tag="VF517_11150"
     CDS             15309..15698
                     /locus_tag="VF517_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8053539.1"
                     /translation="MSYTIRNLRDVPDMAKKFGHSDSQEAHFAHKELGAETVGFSFQV
                     VKPGKRQAFAHRHEQAEEIYVVIAGSGRVKLDDEAQEVRALDAIRIAPGVVRTFEAGD
                     EGLELIVFGPRHEGEVEIITEDVWGDA"
     gene            complement(15695..17833)
                     /locus_tag="VF517_11155"
     CDS             complement(15695..17833)
                     /locus_tag="VF517_11155"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8053540.1"
                     /translation="MNRLTDTVLRHKRMVAAAWILLTLIGMAAAGPASEALDQRFSVP
                     GREGWEASQEILRLYGNGGESLPFVAVAKLPEGSTATSPGVRAELTRVEEVARKSVPR
                     ARIAGYGSTGDKAFVSEDGRTAFVYVFPGRSDDPFGANVETMRDYRDALKDVKVAGAP
                     VLLTGYDALFDSSGEGAEGPGLLLEALIGGAGALLVLAFVFGSWLAVVPLAMAIASVL
                     VSFALLLALTTITEVSPIVQFLVALIGLGISIDYALLIVVRWREEREKGLEGDAAVAA
                     AMQTAGRAVVFSGTTVAIGLLALIALPLPFLRSMGYGGMLIPLVATVVAITLLPVILA
                     TIGPRLDRKRIRRRDRSAAFWQRWSEGVVRRRGLAALVAMAILAGLLAVATDIHLGNP
                     DPDTIAKEGKAREGLVALDESGIGKGAIFPVETLVSEADAAKVATAQGQVEGVHGAAA
                     PEGADWRRGGRAIVAAVPHEGDESAAGRDAVQNVIDAGHSASATARVGGSGPLNADFI
                     DAVYGAFPLMIALISIVTFLLLARAFRSLLLPLKAVILNVLSVGAAWGVMTLVWQAGH
                     GSDEIWGIAATGSISSWIPLMVFAFLYGLSMDYEVFILARMREEYDHVGETDKAVVFG
                     LSRTGRLVTSAALILFLTFAAMASGPQTDVKILATGLAAGIIIDATVVRALLVPAVVS
                     MFGRWNWWLPAGAARLLRVRPSPEPATEPA"
     gene            17884..18321
                     /locus_tag="VF517_11160"
     CDS             17884..18321
                     /locus_tag="VF517_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8053541.1"
                     /translation="MKFPDHGAVNAQTGFLLRKVSTASFNAFAEIVAEHDLHPMHFGM
                     LTILADEEPIAQHELGEKTGVDPSTMVARMDALEERGLIERMRSAQDRRSYEIRLSPE
                     GRRCLAQLRRKAKAHGDRFFAALSESERAQLHRLLAKLAASLD"
     gene            complement(18328..19434)
                     /locus_tag="VF517_11165"
     CDS             complement(18328..19434)
                     /locus_tag="VF517_11165"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8053542.1"
                     /translation="MPTSSTSADEQRLLEQASAGDQDAFGRLVEPYRGELHAHCYRML
                     GSTHDAEDALQEALLRAWRALARFEGRSALRSWLYTIATNTCLNAIAKRPKRVLPVDY
                     APASDPHTAPGEPIVETVWIEPYPDEMLGLEDGLAGPDARYEQRESIELAFVAALQHL
                     PPNQRAVLILREVLGFSAKEVAEALETSVASVNSALQRARAAVDERVPDQSQQQTLRN
                     LGDDELRELVARYVDAWERDDVETFAGMLAEDATFAMPPLASWYQGREGIAAWAARWS
                     MNGLWRWRTVVTRANGQPAIGFYAWDESAQAHVPFALNVLTLRGREVSNVTAFVARTI
                     EGDDNDYSRWPEEAADPRRLQSTFSSFGLPDRLD"
     gene            19509..19871
                     /locus_tag="VF517_11170"
     CDS             19509..19871
                     /locus_tag="VF517_11170"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8053543.1"
                     /translation="MMAATSRPVVHLELHTRDLAGAQAFYGELFGWKPERVETPHGSY
                     VALGLGGELGGGMVQCQTPRSLWLPYVEVGDIEEATGRAQDLGATVLLDPREGPAGWR
                     SVVVTPAGGEIAFWQQKR"
     gene            19915..21138
                     /locus_tag="VF517_11175"
     CDS             19915..21138
                     /locus_tag="VF517_11175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8053544.1"
                     /translation="MGVEGSGLTAGGGRERFLAGDPIRGVAAVLVVVYHTAFLAQPPQ
                     GESLADLGLVYGAAAGDALRALDLTVFVFLVLSGYLIGRPFVHSFVAGRPMPPVGAYV
                     RNRVLRIVPTFWVVFGVVVLLHGLSNYPAGDVLSVFAFAQNYHPSEVAVLIGPGWTVD
                     VEVTFYLALPLVALVLIGLGRTRGTPGARRAVLLGLLAMVYLASLMAKDFDPPGGTLT
                     LITVGFGFMPGIALAALETTPWARTLALRAARNWSLGLVAVAVVVLVPYAISGDPGHY
                     PPRPETAARVMASVFACAVVAAPLVLQWGGGGCPSAVDNRVTRWLGARSYPIYLVHQG
                     ILLEFVGWSVLSERAWPHLALVLAIAMPLVLAAGALLHVAVERPALRRRRDWRAAHEL
                     RGRLGRGRAAPRIGD"
     gene            21200..21607
                     /locus_tag="VF517_11180"
     CDS             21200..21607
                     /locus_tag="VF517_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053545.1"
                     /translation="MAQEIEGLFEPSRYELQAKDLEITYTQLVGGVPDVTIKEGGVEK
                     RYHGTQVRVDRETGLGRIVTVMTDFMFDGNQTTLSLLLPSVNFGDKAEVPVRTVAIQT
                     VHKGSIGGPQLIDGQLSTYKSISLRGKARRIRG"
     gene            complement(22051..22344)
                     /locus_tag="VF517_11185"
     CDS             complement(22051..22344)
                     /locus_tag="VF517_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053546.1"
                     /translation="MGSSEDRGRAKRDALAEAAPQRRVRAATPLTSDVALRLVRAASA
                     ALAPLFLQQGLSLSSTGTHHALLIYLSLYGGAIFFGITFAWTWIALERRKDPG"
     gene            22448..23884
                     /gene="argG"
                     /locus_tag="VF517_11190"
     CDS             22448..23884
                     /gene="argG"
                     /locus_tag="VF517_11190"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF003779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX8053547.1"
                     /translation="MSALNTLEGRAVRGLGCHHDRIRFGWTVVILTSLPAAEQVGVAF
                     SGGLDTSVAVAWIRANGGTPYAYTANLGQYDEPDIASVPERALALGAEAARLVDCRAE
                     LVEEGLLALACGAWHGSTAGKTYFNTTPLGRAVTGTRLLIEMQTDGVDVWSDGSTYKG
                     NDIERFYRYGLLMNPLLRIYKPWLDDRFVSEFGGRAEMSRWLEERGLPCRHSGESAYS
                     TDANIWGATHEAKRLELLDEGMDLIDPIMGVAHWRGNVEIEEETAVVEFECGWPVAIN
                     GCRFGSAIEVVTEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLV
                     TAIHNEDTIDQYRSMGRRLGRLLYEGRWYDPQALMLRDSLQRFVGAPVTGEVAVRLRR
                     GDDYTLERTGGPGLTYRPERLSMERSEGAAFKAADRVGQLKMRNLDIADSRAKLKSLG
                     GSVLANPHTQRLSSAWPPNAPELRTKDG"
     gene            23877..24842
                     /locus_tag="VF517_11195"
     CDS             23877..24842
                     /locus_tag="VF517_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8053548.1"
                     /translation="MAEETLASLLKRFLADRGFRELSSYRVMSRRGGLDDQAIERILS
                     GKTKRPSRAKLLRIVETLRLEQHERNALEAASHHSETHPGFLTVTRAERVPAPGQEPA
                     LGPGRVREGPMWMQRGVILLTAFGDPSDLIKEYTKKSGPWGNVAWRSGVVFGPYDVVV
                     RATTPKGKSVMDFAEQLFEDGEDPDERWMVRTIETIPLRDDMPFYLDPAPSHDHLTPN
                     CVWATIFIQALGGERQPEFPQLFYERANQPDFWGGVHFLTGAVTIGQYDSVVEIIAGN
                     VNILKEYVRAAQKHAATKPYDRHAHTITYYTHQPDLRQGATPATF"
     gene            complement(24863..24951)
                     /locus_tag="VF517_11200"
     tRNA            complement(24863..24951)
                     /locus_tag="VF517_11200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24915..24917),aa:Ser,seq:tga)
     gene            complement(25013..26587)
                     /locus_tag="VF517_11205"
     CDS             complement(25013..26587)
                     /locus_tag="VF517_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053549.1"
                     /translation="MFALVLALLLLAVPPAFAQNADYVPTGEIVADSGFRPTEDGFSF
                     ENYGESQRDLGPNEMRALFGSRACIRVVDGRCTLTQVGREWMNDVNEAMQGGHCYGFA
                     MMSLLLYKDTLPGTRQVGMPTGPTYGMRLNPMLQRNIAYTWAWQTLDSVVDRTVAATP
                     KEVLAQLIEKLRDGGREGWGLGFFGRDGGGHEVTPYAVESKGNGQYVILVYDNNWPGR
                     TSPFYVDTVADKWHYDLSAGNPEFEPDVWDGDAKTKTMDISPATPGIGVQPCPFCEGS
                     KTQFNRVTLDGSPTNHPNLLIQDRYKRRVGWVGDKLVNEIPGAKVIKPVTAEPWQDKP
                     EPEYRIPVSGGGLTITIDGRRLKKRSKGDVRVIGPGYFSAVEDISVTPGDVDTLTVGA
                     RGERLRYKTKRTESPNVGLAFDQGGVSWSFSALSYDVKNRSVIDFRVDKRHGRVHFSN
                     DGGQGNYSIAAFRDGRKTSLDWGATFEITGAVEANIAYNRRIRDDNGKSGLPITWTED
                     DGRTYTDALDRDYQDK"
     gene            complement(26687..27934)
                     /gene="serS"
                     /locus_tag="VF517_11210"
     CDS             complement(26687..27934)
                     /gene="serS"
                     /locus_tag="VF517_11210"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX8053550.1"
                     /translation="MLDLRRIREEPDVVREALERRHVPGGIVDDVLALDARRRELLPE
                     LEGMRGEQKRASEAIAAAKRSGEDAAGAIEAMRGVAGRIKEMGAEVGAVEERLDATLA
                     TLPNLADPTAPPEDDVVREWGEAGAAGRDHLELLGWMVDMEAGARLSGSRFSYLRGPL
                     VMLELALVRWVLDVLTAKGFEPVIPPVLVREEALFGTGFLPDTEDQIYRVPDDDLFLV
                     GTSEVALASLHAGQILEPGALPRRYAGFSPCFRREAGAAGKDTRGIFRVHQFDKVEMF
                     AFVEPEESEDEHERLLAIEEEIYQALEIPYRVVNIAAWDLGASAAKKYDIEGWFPGQE
                     RYRELTSCSNTTDYQARRLDARYRTAEGAPPRHVHTLNGTAIGVQRTIIALAENGQQD
                     DGTIRLPEALHAFGAPKTIGPVG"
     gene            complement(27957..28388)
                     /locus_tag="VF517_11215"
     CDS             complement(27957..28388)
                     /locus_tag="VF517_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8053551.1"
                     /translation="MSLPRPLLACAAAALAALAAGCGGKQDIGVPEGQTTLRHGAELF
                     SERCSGCHHLDEAKARGSKPEDEVAGGERTNGPNFNVRKENRDDVLFAIRNGGFSGAI
                     MPANIVVGKDAEDVARFVEQYAGRKAPTPDEAQEETSQGGE"
     gene            28411..28800
                     /locus_tag="VF517_11220"
     CDS             28411..28800
                     /locus_tag="VF517_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX8053552.1"
                     /translation="MRRAPLAIAAIAFALAGCGDDESGSKGSLTLNSGQPVPVKAYEY
                     GFEPGTITIRAKNTEVAVQFRLTNVGTLPHDIHVRKGDEELGGAEAIGDGKSTSATVS
                     VPPGEYEFYCSIGDHADLGMKGKLTVE"
     gene            complement(28928..29332)
                     /locus_tag="VF517_11225"
     CDS             complement(28928..29332)
                     /locus_tag="VF517_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053553.1"
                     /translation="MGFAVLALNALAAAWGAIAWSRRDPSTGFWYVLRAAQASVVIQA
                     ALGGVLLASGEHVDGLHLFYGLTPLFVSLVTEGMRVGAAQAEVAEVEDVEALERREQV
                     LLARRVVLREMGVMTTGALMIVTLSLRAAGIF"
     gene            29415..29771
                     /locus_tag="VF517_11230"
     CDS             29415..29771
                     /locus_tag="VF517_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX8053554.1"
                     /translation="MLKSYLPALIFVLLGAGLGGLFTYLNGVLGPRRPSKVKSQPYEC
                     GLPSEVTSSFRFGISFYLIAMLFILFDIEVVFLYPVAVGLRDFGMFALVEVIVFVALL
                     FAALAFVWRRGALEWK"
     gene            29762..30439
                     /locus_tag="VF517_11235"
     CDS             29762..30439
                     /locus_tag="VF517_11235"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX8053555.1"
                     /translation="MEVTRQKLGGDPSEDFRIKQLRARQMLRGEISDDDLDTYIEERV
                     GLTTLEKAQAWARANAPWPLTFGLACCAIEMISVVGSPRMDAARFGAEVFRASPRQAD
                     MIILSGRVSIKMAPVIRRIYDQMLEPKWVIAMGVCSSSAGMFNNYAVVQGADKFLPVD
                     IYIPGCPPRPEALMYGIIKLQSQIKGQPDLGWRERYQARGTEEKGLAYERTEADERSR
                     DTGRPLA"
     gene            30432..30953
                     /locus_tag="VF517_11240"
     CDS             30432..30953
                     /locus_tag="VF517_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX8053556.1"
                     /translation="MPDATGLELIAGVVRDRLGEDAVVGTTLYREQATLEVAPPAVHD
                     VLALLRDDEDEAFPHLMSLHGCDYLPEEPRLGVHYQLLSRERLDRLGVKVRVGTDDPV
                     VPSVVDLFPGADFQEREVFDFFGVHFDGHPDLRRILMPEDYEGHPQRRDFPIGGEPVL
                     FTFNEHDMPRWYD"
     gene            30946..32277
                     /gene="nuoD"
                     /locus_tag="VF517_11245"
     CDS             30946..32277
                     /gene="nuoD"
                     /locus_tag="VF517_11245"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX8053557.1"
                     /translation="MTDDRSAVPTQDPSKAPSVVDDGPLSVPYRDRERSVSLERMDKP
                     GAPVFGTELEPETELFTINMGPHHPATHGVLRLLLTLEGEVVRECTPIIGYVHTGIEK
                     SCEDQAYWKVIPYVERMDYLSYNFNATAFCMAVERLLEEEVPPRAQYLRVIMLELNRI
                     ASHLFWLGTSALDVGAISMLWYSLRERDLILDLFEMIAGQRLHLRYIQVGGVIDDPPP
                     GWDRKVRDFCTTMPTRLDQYEAILDRNEIVLQRLKHTGVVPVETMRGLGVTGPLLRAC
                     GDPWDLRKAMPYSSYDHFDFKIPVGTTGDNYDRYRVRMAEMRESVKIIEQALDGLPEG
                     PYITTNRKVALPPRHELATSMEALIHHFKLVTEGFRVPPGEVYMPIESPRGELGCFVL
                     ADGSAKPARVHMRDPSFVNLQSLPHMAKDALIADLIASVAMLDPVLGGIDR"
     gene            32274..32972
                     /locus_tag="VF517_11250"
     CDS             32274..32972
                     /locus_tag="VF517_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX8053558.1"
                     /translation="MSPESQDSHEMDPRVPEGLPVPDDFGRESAMGSRAQDLPRGWDR
                     SYDKDKDPAVIPDIAEVDVPADLRAEIEAHMAKYPDRRSASIPSLHAAQRVHGWCSPL
                     AIRQVAAVMRVTPGYLSSVASFYDMLNEDPVGRHRVYVCTSVACHVVNAKAVYDAIES
                     AGADLPDTEIRQFECLGACDMAPMASVDGRYIGPLDPSDAPEIVAAIREGRKPLPGRG
                     LEDEDYRLPWGGRA"
     gene            32954..34300
                     /gene="nuoF"
                     /locus_tag="VF517_11255"
     CDS             32954..34300
                     /gene="nuoF"
                     /locus_tag="VF517_11255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX8053559.1"
                     /translation="MGRAGLMEFDSLRLIRNINDPELRTIDGYERGGGYRAIRKALLE
                     LEPEQVLHELEESGLRGRGGAGFAMGKKASFIPKGTMDKYLCCNADESEPGTFKDRLL
                     MQKNPHLLIEGIVIAAVAAGANKAFIYIRGEYELQADTLDRAVAEAYERGYLGERILD
                     SDHSIDLVVHRGAGAYICGEETALLDSLEGKRGNPRLKPPFPANQGLYQGPTLINNVE
                     TLCNVPAIIENGADWFKSVGTEKSAGTKLVSVSGNVRRPGNYEIPLGMRAADIVYGLA
                     GGPPEGRRIKCWFPGGSSAPVLLEEDLDLDYDFETMAAAGSMLGSGAIIVVDDSQPIV
                     GLALRLAEFYRHESCGKCTPCREGTNWTVKMLERIDTGEATPADLDVMAEVQTNIMGN
                     CLCVLGDSMAMPIGSMIEKFRDEFERHIEEARLRNNGLPLAAHGGRADLAVGSPAA"
     gene            34303..36885
                     /gene="nuoG"
                     /locus_tag="VF517_11260"
     CDS             34303..36885
                     /gene="nuoG"
                     /locus_tag="VF517_11260"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX8053560.1"
                     /translation="MPRPEPKFVTFEVDGREVSAPEGAMLVDGAKYGDVEIPVFCYEP
                     KLGAPVGACRMCLVEIEGIPKLQTACSTPVKDGMVVTTTSDRVKHAQNAVVEFLLVNH
                     PLDCPVCDKGGECPLQDITFGWGAGRSRFIEPKRHFKKPLELSPLVHIDRERCILCYR
                     CVRFSQEISEDYQLVLVERGDHTFVGTHDGHPYVGPFSGNITQLCPVGALLPSTYRFR
                     ARPWDIENAGTVCTMCPSQCNVELTVRDDKKVLRVNARDNHEVDDGWLCDKGRYGFGV
                     FNAPERIVAPMVRDGGELREVSWERAIGDAVKALDRAGEKTAAIVGGEATNEEGFLLQ
                     FLMREVLGSSHVDSRIGGTLDPEQARQLARPDLAAQVSDLDYAGAVLVLGTELVDEAP
                     ILDLRVRKAVRRRGVPLIVATSRPSTLDPNADVVLRYAPGAEEALVGALAQALGGSGS
                     ESGSGAQPQRSEGWDKVRFAPEPGQPQEPQQQEPEPAVPTDFAERAGASRDAIARAAG
                     KLRGNGPVVVLWGERLSGGERGAQATRALLALTRALDIGSSEGAGLIEIPAGTNGRGL
                     REVGCVPNMKAGLADIEPAGHDAAAIAKALGDEVSTLVLLQADPLRTHPNRPDWERGL
                     DAANTVIAFSDFADETIQNHADIVFPAEGYAEKEGTVTHPDGRLQRVRASVAKPELVR
                     SQTDVLLELLSALSGAPFRLSPPQLTSFMADTVPFYRGLTVDEIGGRGVRWQERDAAA
                     QLPQTPLPETDLEAPPELPGGLRLGTVPSLWASSETEHARALRFLAPTQRAELSPDDA
                     RNLGVEPGDRVEVAVNGTSVGATAALRSGVPAGSVFLIEGTTEDNATALANGKPRVVE
                     VRKA"
     gene            36882..37901
                     /gene="nuoH"
                     /locus_tag="VF517_11265"
     CDS             36882..37901
                     /gene="nuoH"
                     /locus_tag="VF517_11265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX8053561.1"
                     /translation="MTQLAAVGFEESVLVQIVKALLIFLFVLQAVPLILLLERKALGR
                     FQARIGPNRVGPYGLMQPLADVLKLLSKEQFTPATAVPWMMAIAPAISIFTAVVTLAI
                     IPFGDTREGGLFGADYGLYGIDVSIGILWAFAFGALAFYGLMLGGWASGSKYSVLGAM
                     RSAAQLISYEVSLALALLGVVITAGSLSLTEIVHAQDGIWYIVPQFVGFLIFMVAGFA
                     ETNRPPFDLPEADAEIVAGYNTEFGGMRFGSFFMAEYINMIAISGIATAMFLGGWHGP
                     GPGFLDPVWVLAKMFFFIWIFIWIRATLPRLRYDQLMAFGWKILLPLATLNALVTAIV
                     VVATE"
     gene            38069..38605
                     /gene="nuoI"
                     /locus_tag="VF517_11270"
     CDS             38069..38605
                     /gene="nuoI"
                     /locus_tag="VF517_11270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX8053562.1"
                     /translation="MSPWNPDEDVIIVQRGPEPGGVGGAYRAFGETLRGLKTTMGRLL
                     EGPVTREYPEDKTPVYPRFRGRHKLHRFEDSDLEKCVGCSLCAAACPAECIRVVAAEN
                     TPDNQVSAGERYAVVYEINMSRCIFCGYCEIACPFDAITMGNDYELADYTRSDLIFTK
                     EMLLADPIERAPLRREGE"
     gene            38610..39302
                     /locus_tag="VF517_11275"
     CDS             38610..39302
                     /locus_tag="VF517_11275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX8053563.1"
                     /translation="MEELLFFVAALGAIGGALGVVLLRNPFFSVLALVAHLVALALLF
                     LLLNAEFLAASQLVVYAGAVMVLYVFVVAYIGGVDDPGLDQGGAVGRLGPPFAVALLV
                     ELCIAIVGSGLAALDTAGADVGAGFGSPGEVGKLLLEKFLVPFEAASFLLLVAAVGAI
                     VLARRRRGLVDDAEDLPERAGKAPIGGRDGGVRGMDQYDEQPPAGTAEGAGDPTPARG
                     DDRGGTPEEVPA"
     gene            39303..39602
                     /gene="nuoK"
                     /locus_tag="VF517_11280"
     CDS             39303..39602
                     /gene="nuoK"
                     /locus_tag="VF517_11280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX8053564.1"
                     /translation="MDIAWFVVLSAFIFCIGAAGVLTRRNPLVVLLCLELMLNAGNLA
                     LISFSRMLGDHEGQVFALIVMVVAACEVVIGLGLIVAMYRNKIPIDVDEVRELRG"
     gene            39599..>39852
                     /locus_tag="VF517_11285"
     CDS             39599..>39852
                     /locus_tag="VF517_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053565.1"
                     /translation="MSAGGWGWLILLFPLWGTILISLFWRLLPRRTAGWLGSAAILGS
                     FVASLGAFFSIQSDDVHHRVVESNAFDYVKTAGVNVDLGIL"
BASE COUNT         5962 a        14021 c        14047 g         5822 t
ORIGIN      
        1 cggcccgcga tgccgacctt gcgcccgcag gagctgtccg gactcgtggt catccccacg
       61 gtggagcacg accccggcat ccaccgcgtc tcgatcgcga tcgacgtggg cgtgtcgcga
      121 acaggggcgg ggatcgcacc cccggaggtg gtcaccaggg aggacctggt cgtggtgctg
      181 cgcaacgcca gcgaggggag cctcgaggcg atagcgagcc cggaccccgg ggccctcccc
      241 gtccgcgcga ttcgggtcgt gcaggctcgg ggcgagttca cgttctcgca gggcgtgaac
      301 ccgccggacg agctggtcgt cacgctgcga ggcgatcaga agacgttccc gatgtcccag
      361 acgctctcgc cgacccgctg cctgggcaag gagccgcagg tcggggaccg ggccccgggc
      421 ctaccccgga agggcagctt cctccatcgc cttccgttca tccaacgact gcgccgctcg
      481 cggtgctgcg tcaagcgact ggacgctccg gtcaacccca gcgctgacgc gaccgccaag
      541 agcgagcact tcgaggtgga ggccgacttc gtcgcgcgcg gtgccaagtg ccagtgcggg
      601 tgctgcgagt accggcagta cgttcgaggg accttcagcg acgccgccgg cgccccggtc
      661 cggttcgatc tgccctcggg cgcactcgac cccgccagct actgcgagga cggcgcgatc
      721 gacgagttcg gcccgggcgc gcacggctat tacgggcacc ggagcacgag ctcgccggga
      781 gacgcctacg cgggcgcggg caagtcggcg agctgcacat accgcgggaa cgagaggccg
      841 agctgtcctc ccacggacgg cgtccacctg gagttcctcg gcctggtaat cgatgtctgc
      901 caggggcgcg tggccgcgaa gcggacctgg atcgtcgacc tctagtcccg ggtctgcctc
      961 cgggtcaggc ccggatcatc ccgagctcgg cccgttcgtc gccagctcga agtgcgactg
     1021 gatcgtggcg aagtcgaggt cgacgttgta gaccgccacc tcgtcgacca cgccgttgaa
     1081 gaggtgctgc ggagtatcgg actcgttccc gcccgcacct atgcggaagt cccgagccgt
     1141 attcggctgg tagaccacct ctgccgtggc gggcgcgtcc gcggccgggt cgacatacag
     1201 cttgagcgtc ttcccgtcgt aggtcatcgc caggtagtgc ctcccgggcg ttgcgtcggg
     1261 cccacccgcg gcgaccccca cgcccgacgc gccatccccg acccacgcct cccacttgtt
     1321 ctcgttgttc acgtagatcg cgtatccgaa gatctgcgtg tccgggctga tgtcgcgcga
     1381 ggaggcgacg gcacggtgac cctcgccgcc ggcgacatcg gcgatcgcct ccacggtgaa
     1441 cttggccggg ttcaacgcgt cgctgtgagg cacggtcacg tggcccgtgg acccgtcgaa
     1501 ctgcgcggct gtgtcgctgt cacccaccac gaaccccggc tgtccgagcg tcacacctcc
     1561 gacgtacgtc ccgttgtggc cccccttgct gtcgaccgcc gtcgtggctc ccacggcctc
     1621 accgagacgc cagtagccga cgaggttcgt gttgttcgtg acgctgtcct tgtactgctc
     1681 gggtccgccg aggcccccgg tctccaactg gcagccgacg aaggcgaaca acatcacgaa
     1741 cgacgccagt gccagcggcg cgactagaag gatccagtgc gacattccag ctcctttcgg
     1801 gtcccatgcg tctcccgcag gggattgagg atcgagcgca cgcagccgta gccggccagc
     1861 gcgaagacga agatggcgcc cgggaacggc ggggcggtgg cgatcgccgc ggcgccggcg
     1921 agcagcgtca cggccgccgc cagctcgagg agctggacgg ggtggcgccg ctcccagcgg
     1981 cccgcgtgat cgggcaggtg aagggcgacg cgaccgtcgc tcggccggcc cgcgcagcag
     2041 ccgttgagga ggcagccgac gcgggtgaag accatgccga ccagcatcgt gaacgtcgcc
     2101 acgtcccaga aggcggcgaa cgggagcccg agccccgcga gcaccgccgg cgagaccgcg
     2161 acggcgagga gcagcccacc cacgagggcg gcgccgccgt ccccgcggcg ccagatccgc
     2221 cgcggctcac gacggaacac ctcccagtga ctcagcacga accagagccg cgagcccacg
     2281 agagccggcg cgatcaggat gaggagcgcg agggacgcgc ggtcggggtc gatcggcatg
     2341 ctcgtggcca cgccatagcc ggcatagacg ccgcagagca acccgacgta cagcatcgcc
     2401 ggatagctgg ggacgttgac gccgtgccag ctgaagagga cgcggcgcac cgtcagacga
     2461 ccgagccgac cgcgacggcg actccggcga cgatcgccac gtccgcgagg ttgaaggccg
     2521 gccaccagcc gagagcgatg aagtcgatga ccgcaccccg accggcccgg tcgagcaggt
     2581 tgctcgtagc gcccccgagc gccacgacga gtccagcgcc cgcgagtccg tgtgagggcg
     2641 accccgcgcc cgtggcaagc acgagcaccg tgcacgcaag cgtcatgctc cagagcccga
     2701 gctgagccgg cagggacggc gcgcgaccga gccacgagcc cccgttggcc gcgagcctga
     2761 acactcccga ccgcctggct ccacccactc cggcggaggc gaggaaccgg accgcgagcg
     2821 acttcgtcac ctggtcgaga aggaccaccg cgccggctcc tgcaagcacc cagctcgcca
     2881 cgcgggcgat cttaaccgct caggaggccc ccgcgtcaat tgttgcgccg atgcccttga
     2941 cgtacagcgc atagccgttg cggcgcgccc tcgccaaccg gaagccgtgg ttctcgagcg
     3001 cgcggcgagc gatcgcgtgg cgggtcccgg tgtgggccac catgtgccgg aagccgcgtg
     3061 cctcggccgc gatctcggcg tggtgggcca gcgtccccgc gacaccgcgc cagcgtgcgt
     3121 gccgggcgac cgcgatcgtc ccgatgtaat acgcgccgcg tggaggccgc gggctcgcca
     3181 ggacgatctg cggcagggcg gcggcgagca gcggccaccg cccgggcctc aggtcacgga
     3241 ggctcgtgag cagcagcgcg cgatggaggc ggtagcgatc gcgcaccggg aagccgatca
     3301 gcacgccgac ggcctggcca tcgatctcgg cgaccagcgc gtgctcgaag ctcgcggagt
     3361 ggcccggctg cgaccacacc cgagcgacga tgcgccggcc gtcgtcggcg gatccggcca
     3421 gacgcgtgta cgcggtggcc gcggcatcga atacgagtcc cgccgcggcg atgtcgtcag
     3481 ctcgggccgg acgaaccgcc acacccgaag cgggaacccg tgaacccgtc acgagcggat
     3541 cctaggtcgg ctctcctcag gcggttcccg aggccagcac acaacgacca ccaactgacc
     3601 gagttcgatc aaaaattccc cagcgcgctg gtacaatcgg tcagtgcgcc gccccctcgc
     3661 cgcactcgca ctcgccgccg ccggcctcct cgccgtgccg gcggtcgcac aggtgcccgg
     3721 cgacccggtc agggtcgagc cgagcacggc cggcacgagc tcccacatga tcgtggacat
     3781 ccacgggtct gccgacccga aggcgaacgg gcgccccgcg acacacgccg taattgacct
     3841 ggcccagggc ttcaggcgcg acctccgcgc gcggtcggaa cgttgcacgg agtcgcgggc
     3901 caaggcgttc gactgccccg gcaagagcaa gatcggctcc ggcacggccg acgccacggt
     3961 gtccaatggc gcgatcagcc aggccgtggt ggcggacgtg tcgctcttcc tcgcaccacg
     4021 cgcccagtcc agcgacgcgg ccggtgtgtg gctcatcttc acggagcgca gcagcggctc
     4081 gcgggcctgg atcttcggcc ggctcctcaa gctcggcagc cacggcccgt tcgggatcga
     4141 ggcgcggttc gagaacttcg agaccgcgaa ctcggccgcg ccggagggct tcaccgtgcg
     4201 gctcgaccgc atccaggtcg aggtgggcgc gtcccgcacc gagaagatca cggtctgctg
     4261 caagacggtg cggcgcaacg gcaagaagaa gaaggtcagg tacaggaaga aggtcgtccg
     4321 taacctgctc cacaacccga agagttgcga cggctcgtgg gactaccagg tacggatccg
     4381 ctactcggcg accgacgaga gcgtgcgcga cggcgtcatg ccttgcacga acccctagta
     4441 ggcgcctgcg aaaccgcggt gggtacctcg agcgatccgc gcgctctggc tagctgctgc
     4501 tctcagccgt ggggccgtgg ccggggatga ggccagaaac gacaccgacc ccgtctcagg
     4561 ggccggtgtt cgcacttcga tcaaatggcg gagagggcgg gattcgaacc cgcgaaggag
     4621 cttgcacccc ttactcgctt agcaggcgag tgccttcagc cactcggcca cctctccgga
     4681 ccgggccgat tctacggctc gcgcggcggc gcgtcagccg cgcggcaggc gcttcagcgg
     4741 atcctgcggc tcgccccaag gcgcgcggcc gctgcgcacc tcgaagtgca ggtgcgccgt
     4801 gtccgaggtg ccggtgtccc cgacgaaccc gatcacctcg ccctgcgaca ccttggctcc
     4861 ctgcttgacc cggaagcgcg actggtgcgc gtaacaggtg acgaacccgc ccgcgtgcgc
     4921 gagacacgtg tagttgccgt agccgtcctg ccactcgcgc atcacgacga caccgtccag
     4981 tgccgccctg atcggcgtcc cgtcggcgat cgggatgtcg attccggcgt gccggtggcc
     5041 gccgcgctgc tctccgaacc gcccggtgat cgccccgcgc accggccaga tcaggccgga
     5101 ggcccgcgcg cgccgcggcg cggccggctc gtactggagg ccgatctgcg aggcgccgat
     5161 caccgcggct gcgtcaccgt taccgccgtc gccgctcgcg cccgtcgcca ccgcagcgcc
     5221 gccatacaga gcggccgcga gcatcgcgat aactgcaaac ccggcgaatg agcgcactgc
     5281 gtgaagctcc cctgtctcgg cctacggggt tagctgacgg gctcgcgctc aaggcagcgc
     5341 taccgggcgg tactgcccgg attcgcccca cgccgccata tggcggcact ggttcccccg
     5401 atcccacacg cgtgcgtgcg gcattcggca catctacgaa gtcgaggagt gtatttcccg
     5461 accggatgga tgcaacaaac gtctcaggca gactctctta catggaccgc cgcttgctct
     5521 acgccctgat cgcactcgcc accgtgctga tcgcgctcgt cgcgacgcag gtgctcgtgc
     5581 cctcgtatct cgagcgccga gccgagaacc ggctcaccga ggacggcggc aaggcgaccg
     5641 tcgacatcga cgcgctcccc gcgctgggcc tcctgttcga ccacggcaag cgcatccagg
     5701 tgcgcggcga ggagctcgtg atcccgctcg agggcgagcg cggcaaggtg ttcggcgacc
     5761 tcgaccgctt cgccgaggcc gacgtgcgcc tcgaccgcat ggtcgccggc ccgctgcgca
     5821 tcgcgcgttt ctcactcgcg cgcgggaagc acggcgaccc atacgccctg accgtcagcg
     5881 cgaccgtgac cgcggccgag ctcggcgact acgcgggcca gcagctcggg ccgctcgccg
     5941 gcttcctcgg ccgcatcggc gcctcggtgc ttccgttctc gaacgcgccg atccccgtcg
     6001 agctcgacgc ctcgctgaag agcgacgacg gccgcgcggt ggtcgagacc gtcgacggca
     6061 ccgtggccgg gctccccgcc gaccccctgg tcggcgccct cgccgaggcg atcgccgccc
     6121 ggctctgagc cggtacgctc gcgccgtgga cgaggaggac ccgacgacgc aggagctgcg
     6181 ggtggagcag ctccagcgcg agcgcatcga gcgcgaggcc gcggcggccg gcgcgacgcc
     6241 ggacgacacc gagcagcacg agcggcgtgc cgaccgggca agctacctgc gcgagaagct
     6301 cgaggagcgg gccgaggccg aacgcgacgc cgcccgcgaa gacgacgact gagactggag
     6361 acacgatgga gtacgacgag cgggctgacg agctggagaa ggaggcggag cgcctagagc
     6421 acgagtcgaa gcgcctcggc gagcggaccg acgcggcccg ctcggagtgg gagtcggcga
     6481 agtcggatcc gagcaaggcg cccggcgcgc aggccgccga ggcgaccggc ccgcaccaca
     6541 tcgacgccga ggatcccgcc accgggcgca ggtacggcga ggtccgccaa gaggagatcg
     6601 aggacgccga ggcggccgac tccgagtaac cgagataacg actcaacctc tggcgcgtta
     6661 cagcgttgac cccgtagacg catgggaggc gtcgggcggg gggagagatt gaggacgctt
     6721 tgctcggacg acatcaacgg aatgaggatc gggagccgga tcgcccccgg ggcggcaccc
     6781 tcggccaggc gatagccgcc gcgcgcgacc agctcgggct cgagatcgtc tgcgtctcgc
     6841 gcaccgtgcg cgcgagcgac ctgagcgacc acgcgggcga cgcatgcggg gtggacccgc
     6901 tcgcggacgg atcggccatc gtgccgctcg agtccggcga cgggcacctg cacgggagcc
     6961 tggtgtgccg gccggtcgac ggccacgagc tggacgaccg cgacctcggg ttcctgcgcg
     7021 tgctcgggcg cgtggtgggg acgcagatcg cccacagcga gcgcgatcgc aagctgcgcc
     7081 gccagcaggc cgagaccacc gggctccagg cgctgctcgc ggccatcgag gcccgcgaca
     7141 gctacaccgg cgagcactcg cgcagcgtgg tggatctctc cgtgcgcgtc gcgcgcgagc
     7201 tccagctcgg cgggcgcgac ctcgaccagg tcgagcaggt cgcgctgttg cacgacgtcg
     7261 gcaaggtcgc ggtgccggac gcggtgcttc agaagaacgg cccgctcacc gagcccgagg
     7321 tcgagctggt acggcgccac ccggtggtgg gtgcgcgcat cgtcagctcg gtgtccgagc
     7381 tggcccacct cgcgcccgcg atccgctcgg gccacgaacg ctgggacggc cgcggctacc
     7441 ccgacggcct gagcggcgac gagatcccga tcctcagccg catcaccttc gtgtgcgacg
     7501 cctacgatgc gatggtctcg gaccgcccgt accgcgacgc gctgccgccc caggtcgcca
     7561 tcaccgaggt cgagcgcaac gccgggtcgc agttctgccc gcgcgcctcg gcggcgctgc
     7621 tcggagtgct cgagaccacc cgctcgcacg cacgctcggc aacgcgcttc ttcaccgcac
     7681 cgccggttcc gtaacgcgtc gatacagtcg cgtccggcat gcagacgaac gagctgaaca
     7741 gcgacctgct gcggcgtctc gccgacctgc ggcccgaggg cgcgcgggtc ctgagcctct
     7801 tcctcaacct cgacccgtcg gagttcgcaa cgccccccgc gcgtgccacc gaggcgcgct
     7861 cgctgatcga ccaggccgag cgccagctcc gtgagtccgg cgagctcgac cacgacgccg
     7921 agaaggcgct gcgccaggac atcgagcgcg cgcgcgagct cttcgaccgc ctcgacgccg
     7981 acggcgcgca cgggctggcc gtgtacgtct gcggccccgc cgacctgttc gaggcgatca
     8041 agctgccgcg cccgatcgag acccgcgcgg tcgttgacga ctcaccgttc gtggagccgc
     8101 tcgccgagct cggcatgcgc gggagctggt gcgtgatggt cgtgaaccgg caggtcgcgc
     8161 gcatgctgcg cggatcgccg gaccagctcg aggagctgcc ggtggtcgag gacgaggtgc
     8221 acggccgcca cgaccagggc ggctggtccc aggcgcgcta ccagcgcagc gtcgacgagg
     8281 aggtgcgctg gcacctcaag cacgccgccg aggtggcctt ccgccgcttc aagcgctcac
     8341 cgttcgacaa cgtcctgctc ggcgggccgg aggagacggt caccgagttc gagggcatcc
     8401 tccaccccta cctgcgcgag cgcgtggcgg gccacttcaa ggtcgacgtg gagaacacca
     8461 cgcccgacca gatccgcgcc gccgcggcgc cgctgatcga cgcggtcgac gagaagcgcg
     8521 agcgcgaggc gctcgaccga ctcgaggagg gcgtcggcac cggtggacgc gggacggccg
     8581 ggctggacct caccatcgcc gccctcaacg agcggcgcgt cgagatcttg ctgctgagcg
     8641 accgtttcga cgccgcgggg gtgtactgcc gcggctgcgg gtgggtcggc accgacgtga
     8701 cgctgaccgc ctgccccgtc gacggcggaa ccctcgaaga gcgcgagaac atcgccgaga
     8761 acgcgatcga gctcgcgatc ctccagtccg cgcaggtcct gcgcgtgcgc caccaccccg
     8821 agcggctcga ggcccacggg tccatcggcg cggtgctgcg cttctagcgc ggcggcagcg
     8881 tcgacacgcc gggcagcagt ccccgccagc gcagcgccgc gcgcgcggcg ttcgcgccgc
     8941 aggcgccgtg gaccgctccg cccggatgcg cggaggagga gccgaggtag aggttgcgga
     9001 tcggcgtctc cggacggccg acgcccgagg tcggccggaa cacgagctgc tggtgcgcct
     9061 gcgccgtgcc gccgttgatc gccccgttca cgaggttgga gttcgcgcgc tcgaggtcgt
     9121 gcggggtgaa cacgtgccgg gcgcggatca gatcgcggaa gccgggcgca cgcgcctcga
     9181 tctcgtcctc cagccggcgc gcgaacagct cggtctcgcg ctcgtcgaag cggccggtga
     9241 tgtcctgccc gccggcgtcg cccttcggcg tgcgcgggtg gtgcgtgtag gcccaggcca
     9301 cgtcgcagcc ctccggtgcg cgcgtcgaat ccacgggcgc gtactggccc acgaccatca
     9361 gcgggtgcga cggcatcagc ttgcgcgcga gctcggacgc gtggacggtg aagtggtcca
     9421 tgtcctcgcc ggtgtggatc acgcccgagc ggcgggcgtc gggcgccgcc cacgggatcg
     9481 gcccgtcgag cgcccagtcg accttgaagg tcgaggcgtc gtattcgaag cggcgcaggt
     9541 cggcgaccgt gcggttgggc aggtgctcct cgcccaccag gtcgaggaac agcttcggcg
     9601 cgaccacgtc cgcgatgacg gcgcgcgggg cgtcgatctc ggtgccgtcc gcggtgcgca
     9661 ccccgaccgc gcgcccgccg cggacgatca cggcctcgac gcgctgaccg cagcgcacca
     9721 ctcccccgcg cgcgtcgagc cgcttcagca gcgcggcgat gatctggccg gcgcccccct
     9781 cgggcgtggg gaagcccacg tgctgaccga gcaccgacag catccagccg aacccggcgc
     9841 tgaccgccgt ctccggcgtc aggtccgcgt gcagggcgtt gccggtgatc atccggcgcg
     9901 cctgctcgga gacgaaccac tcctcgccga agcgccgcac cgacagggtg atcatgcgca
     9961 ggaagcgaac cgtgtcgcgg ttcagcgccc aggccatctt cgccgccccg cgcagcggcg
    10021 ggaagggcgc gccgaagccg gcctccatga tcgggtcccc cacccgctcc cagatcccgt
    10081 agagccggcg ccaggcctca ccgtcgccgt tctcgtagtg attcagacgc tcgaccgtct
    10141 cctcgagatc gagtgacacg tacgggcagg tcccgtcgat cgccgggtgc gcgaacgcga
    10201 gcggcgagcg ccgccacacg agaccgtgct cctcgagccg catcgcgcgc atggcgggcg
    10261 acaccacggt gagcgggtag aacgcgctga agaggtcgtg gacgaagccc ggctggccgg
    10321 tcagctcgcc gctcttgacg gcgccgcccg gagcgtcctg ctcctcgagc acgagaacgt
    10381 cccagcccgc gtcggctagg acgttggcgg ccacgaggcc gttctgaccc gcaccgatga
    10441 caaccgcgtc agccatgcgg tcgaggctac ccggccgggg gtaggacaac ccggcagccg
    10501 gcggggccgc gagactcgag cgacacccgc cggacaaccg gttgaagcgg gacccacgag
    10561 ggggttgggc cccgccgggg agctgctccc gttagtgggt gcggctcccg aacctgccgc
    10621 cgagcccgta gccgccggtg gggataccgg ccagcagtac gaggatggcg gccacgattc
    10681 cgacgattgc cggaaggccg agtgcaacgc agatcgcgta aacgagggct gcgaagagaa
    10741 cggccaagac gatgccgatc atctggactc cttccctctt ctgtaggggt actgggcagt
    10801 cgattgccct gctcctgttg ttccccttac agaacgcagc tacgcctccc gggttggatg
    10861 gaagcgtcgc ggcgcaattt ccgtcccgcc cgcgatggat cggggacgtt tgcgggaaga
    10921 tacgttccat gccccgatct atatggaccg gcgcgatcag cttcgggctg gtcaacgtcc
    10981 cggtgaagct ctacagcgcg gtctccaaga agaccgtccg tttccatcag ctccacgagc
    11041 ccgacggcgt gcgcatccag cagaagcgct tctgcccggc cgacggcgag gaggttccct
    11101 acgacgagat cgtgaagggc tacgagatca gcccggaccg ctacgtcgtg atctcgccgg
    11161 aggagctcga ctccctggag ccgcgcaaga cgaagaccgt cgacatcgag gacttcgtcg
    11221 acctcgacga gatcgacccg ctctactacg accaccccta ctacctgctt cccggccagg
    11281 gcgcgcagaa gccgtacaag ctgctggtcg aggcgatgaa gcaggcgaac aaggtcgcga
    11341 tcgcgcgcgt ggtgatcagg accaaggagc agctcgtcgc gatccggccg gtgggcgaca
    11401 tcctgtcgat ggcgagcatg aacttcgccg acgaggtgat cgaccccgac tcgttcgacg
    11461 aggcacccgg cgacgacgtc gagacgcgca agcgcgaggt cgagatggcg cagcagctga
    11521 tcgagagcct cacgtccgag ttcgacccgt cgaagtacca cgacacctac cgcgagcgcg
    11581 tgatggagct gatcgaggcg aaggccgacg gtcaggagat cgcggtgcag ccggaggagg
    11641 aggtcgctcc ggtgccggac ctgatggcgg cgctcgaggc gagcctcgcc gcggccaaga
    11701 aggacaaggg aggctcgggc aacggcaagc cgaagtccgc ggagcccaag aagaagccgg
    11761 ccgcttcgaa gaagaaggcg gcggctaaga agtagacctc agacgaggtg ttcgttctaa
    11821 gtcacgcata ccgtgagtta gaacgaacac ctcgtcctcg aggcctactg gtgtgagtcg
    11881 aagccctcgt cggccggctt cgggctgggc ccgccgccgg tctcgtcgcc gtcgccgccg
    11941 tcctcgggcg cggtcggacc gcctatgtcg cgatccgtgc gctcgcccgt gccggccggg
    12001 tggccctcct ccgggctcgt gtgcgggctt ccgccctcgt cctgcttgtc ttgtccttct
    12061 tgatggtcgg tcatagcgcc acccctaccc ggtcgccggg actgcgactc atccgcgacc
    12121 tcctcgcgcg gcccgaacgc cacagctacg gcggctcccg ctcccagcgc gccgacctct
    12181 acgtgcccga cggtgccggg ccgcacccgg tcgtggtgct gctgcacggc ggctactggc
    12241 gctcgaagta cgggaagtcg ctgatgcgcg ccgtctcggc ggacctcgcg cggcgcgggt
    12301 tcgccgcgtg gaacgtcgag taccggcgca tgggccgcca cggcggcggc tggccgatga
    12361 ccttcgacga cgtgggcgcc gccatcgacc tgctgccgga gatcggccgc gggcggctca
    12421 ggctcgacgc ggtcgacgtg gtcgggcact cggccggcgg gcagctggcg atctgggcgg
    12481 cgacgaggcg cgacgggcgc gtgccggtcc ggcgcgtcgt ggcccagtcg gcggtctgcg
    12541 acatggtgtg ggcgggcgcg gccgcacgcg agctcctggg cggcggcccc gacgaggtgc
    12601 cggagcgcta cgtggcgacg aacccgatgc agctcgtccc gatcggcaag ccgacgctgc
    12661 tcgtgcacgg ctccgatgac gagaccgtgc cgctcgagcg atcgcgcctg tactacgagg
    12721 ccgcgcgtgc ggccggcgac ccggtcgagc tgatcgaacc ctcgccgggg caccaccgga
    12781 tccacatcga cccgcgctca cgcgcctggc aggttgtagc ggagtggctc gccgggtagg
    12841 gcatgcccga tgacccgcgt cccggaaccc gtcgccaggg ccaccgccag cgtgttccgg
    12901 accctctcct cgctgcgcgg caagcgcgcg ttccacccgc atgggctggt gttcgaggcg
    12961 gagttcgagc cgtacctgcg ctcgtcgccc accggggtcg agatcctcga cggcgggccg
    13021 cgccgcgccc tcgtgcgctg ctcgcgggcc ctcgggttcc ccgagccggc ccccgacatc
    13081 ctcggcttcg cgatccggtt caccgacgcg tacggcgcgg ggcgccacca ggacctgctc
    13141 tgcgtcacgt cgatcgacgc gccggtgggt caccacgcgc tgatccccgc gaccggcttc
    13201 ttcacgcggc cctactcgac gctgctggcc taccggctgc ccgagggcgt ccggctgttc
    13261 ggcgtgttgc cgatcgggag cgggcccgcg ctcggcccgc ggctcgagca gatcgccgag
    13321 gcggcgcggc gcggcacggc gacgttcgag ctggcgctgg cgcctctgag cggccgcttc
    13381 gacggtgtcg gcacgatcca gctgggcgac cagctcccgc cggagcaggg cgagctgctg
    13441 cgcttcaacc cgtggaacag cggcggcggc atgtgcccga ccggcccgtt catgggcctg
    13501 cgcgacgcgg cctacaaggg cagccaggag ggggcggcga cctagttact cggcggccgc
    13561 ggccttcgcc tcgtcgagcg cggctacgaa gccctccacg tcggcgcggc gctccgccgg
    13621 cacgatcagc gacatgaagt tttggcagac caccgtgatc tggatcagct cctccttctc
    13681 cccgaagaag accttcttgc ggcgggtgaa gtccttgacc tgggtctcct tgtacgagga
    13741 gatggggttg cgggcgcgcg agttgccgat ccggagcttc tcgatccagt tcccgtccag
    13801 cacgatcgac tcgccgtcga tgccctcgag cgtcagcacg ctgcgatcct aacgccgctg
    13861 tgacctccct tccgctcgca cacgagacct ggctgaccca cgacgacgtc gcggtcgact
    13921 gggacttcgt gctcgagggc ctcaccctcg cgctgctcgc cgcggcggtg ctcctcaccc
    13981 tggccgtccg cctcgtagcg cggcgccggc cgggcgtgga cgtgcccgtg ctggcgcgga
    14041 tggcgccctt catgccgttc gccgtgcggc tgcacctggc cgtatcgctg atcgggctcg
    14101 tctcgctcgg ctactacctc tcgccggcga tggacctcca ggccgacctc gccgggatcc
    14161 tgctcggcgc ggtgatggtg atcgtcgccg tggcgatggt catcggcttc caggtccggg
    14221 cggccgcgtg gctcctgatc gcggccggcc cgctcgggat gctcgagttc ggcgtgtcac
    14281 ccgtcgtcca gcggatcgac atgctggggc ccgcgctctt cctgctgttc gccggagccg
    14341 ggcgctggtc ggccgagttc gagctcgggc gcgtgcgcga cccctccccc gaggcgatcg
    14401 cgcgcgcggt gtgggcgcta cgggtcgccg ccggcgtcgc gctgatcgtg gtcgcgttcg
    14461 tcgagaagct cgcccagccg gacctcgcgc tcgagttcct ccatcaccac ccgcagttca
    14521 acgtcgcgcg cgaggtcggg atcccgatgg gcgacctcga gttcgcacgc gtggcgggcg
    14581 cgatcgaggt gctcttcggg ctgctcctga tctccggcgc gctgccgcag gcgatcgtgc
    14641 tgatcgcggg catcccgttc aacgcgacgc tctggttctt cggcaacctc gagctggtgg
    14701 gtcacctccc ggtctacggg accatgctcg tgctgctggt ctacggctcg tcgcccgagc
    14761 ttcgcccgct ggcgtcgcga atgtggccct ggcgccccct ccgcgcctga tccgccagcg
    14821 gccgcacgcc gtatcgaagg cgtggggagc gtgtggggca tcgtcggggc accgctggca
    14881 ctcgcgaagg gtgtggtgag cttcacccgc gacgtgggga cgatcgcgcg ggtcgcgcgc
    14941 gaggccgagc cggtgatccg cgaggtcgag ccgatcgtcc gcgacaactc cacgaccgct
    15001 cgcgaggccc tcgacgtggc acgcgagaac aacgcgctca cccgccaggc gaacgcgacc
    15061 atcgcgaccc tcgatctcca cgccaccgag ctgctgaggc gggtgggcac gatcgagctg
    15121 gagctgccgg cgatccgccg cgggctcgcc cacgtggagg acctcgccga ctcggccgag
    15181 acgctcagcg tggccgcgcc gcccgtgcag gccgccgccg agcgcgtcga gcgactggcg
    15241 caacgcatcc ccggcgcccg gcgctcgacg cggaggctca acggccgcga cgcgtagtct
    15301 cgcgtgccat gagctacacg atcaggaacc tcagggacgt gccggacatg gccaagaagt
    15361 tcggccactc cgattcgcag gaggcgcact tcgcgcacaa ggagttgggc gccgagacgg
    15421 tcggcttctc ctttcaggtc gtcaagccgg gcaagcggca ggccttcgct caccgccacg
    15481 agcaggccga ggagatctac gtcgtgatcg cggggagcgg ccgggtgaag ctcgacgacg
    15541 aggcgcagga ggtccgcgcg ctcgacgcca tccgaatcgc gcccggcgtc gtgcgcacgt
    15601 tcgaggccgg tgacgagggc ctcgagctga tcgtgttcgg cccccgccac gagggcgagg
    15661 tcgagatcat caccgaggac gtctggggcg acgcctaggc gggctcggtc gccggctccg
    15721 gcgaggggcg tacgcgcagc aggcgcgcgg cacccgccgg gagccaccag ttccagcgcc
    15781 cgaacatcga gaccacggcg ggaacgagca gcgcgcgcac gacggtcgcg tcgatgatga
    15841 tcccggccgc caggccggtg gcgaggatct tcacgtcggt ctgcggcccc gacgccatcg
    15901 ccgcgaacgt gaggaagagg atcagcgccg cgctcgtgac gagccggccg gtgcggctca
    15961 gcccgaagac gaccgccttg tcggtctcgc cgacgtggtc gtactcctcg cgcatgcgcg
    16021 cgaggatgaa cacctcgtag tccatcgaga ggccgtacag gaacgcgaac accatcagcg
    16081 ggatccacga ggagatcgag ccggtggcgg cgatccccca gatctcgtcc gatccatggc
    16141 cggcctgcca cacgagcgtc atcacgcccc aggccgcgcc gaccgagagc acgttgagga
    16201 tcacggcctt gagcgggagc agcagggagc ggaacgcgcg ggcgagcagc aggaaggtga
    16261 cgatcgagat cagcgcgatc atcagcggga aggctccgta gacggcgtcg atgaagtcgg
    16321 cgttgagcgg cccgctgccg ccgacgcggg cggtcgccga ggccgagtgg ccggcgtcga
    16381 tcacgttctg tacggcgtcg cggccggcgg cggactcgtc gccctcgtgc gggaccgcgg
    16441 ccacgatggc gcggccgccc ctgcgccagt cggcgccctc cggcgcggcg gcgccgtgca
    16501 cgccctcgac ctggccctgc gcggtggcga ccttggcggc gtcggcctca gacacgagcg
    16561 tctccacggg gaagatcgcg cccttgccga tgccggactc gtcgagggcg acgagtccct
    16621 cgcgtgcctt gccctccttg gcgatcgtgt cggggtcggg gttgccgagg tgaatgtcgg
    16681 tggcgaccgc cagcaggccg gcgaggatcg ccatcgcgac gagggcggcc agcccgcggc
    16741 gccgcacgac gccctccgac cagcgctgcc agaaggccgc gctgcggtca cggcggcgga
    16801 tccgcttgcg gtcgagccgc ggtccgatcg tggcgaggat gaccggcagc agggttatgg
    16861 cgacgaccgt ggcgaccagc gggatcagca tcccgccgta gcccatgctg cgcaggaacg
    16921 gcagcggaag cgcgatcagc gcgagcaggc cgatggcgac cgtcgtgccg gagaagacga
    16981 cggcgcggcc ggccgtctgc atggcggcgg ccacggccgc gtcgccctcg agccccttct
    17041 cccgctcctc gcgccagcgc acgacgatca gaagcgcgta gtcgatcgag atgccgaggc
    17101 cgatcagcgc gacgaggaat tggacgatcg gcgagacctc ggtgatcgtg gtgagcgcga
    17161 ggagcagcgc gaacgagacg agcaccgatg cgatggccat cgcgagcggc accaccgcca
    17221 gccaggagcc gaacacgaag gccagcacga gcagcgcgcc ggcgccgccg atcagagcct
    17281 cgagcaggag gcccgggccc tcggcgccct cgcccgagga gtcgaacagc gcgtcgtagc
    17341 ccgtgagcag gaccggggcg cccgcgacct tcacgtcctt cagcgcgtcg cggtagtcgc
    17401 gcatggtctc gacgttcgcg ccgaacgggt cgtcgctgcg gccggggaag acgtagacga
    17461 acgcggtacg gccgtcctcg gacacgaacg ccttgtcgcc ggtcgagcca tagcccgcga
    17521 tccgggcgcg cggcaccgac ttgcgggcca cctcctcgac gcgcgtgagc tcggcgcgga
    17581 cgccgggcga ggtggcggtg ctgccctccg gaagcttggc gaccgcgacg aacggcaggc
    17641 tctcgccgcc gttgccgtac aggcgcagga tctcctggct ggcctcccag ccctcgcggc
    17701 ccgggacgct gaagcgctgg tcgagcgcct cgctggcggg gcccgccgcc gccatgccga
    17761 tcagggtcag caggatccag gccgcggcga ccatgcgctt gtggcggagg acggtgtctg
    17821 tcagccggtt catcatgggg actattcgta tcacaaatgc ttcgcgttgc gtatgatcgt
    17881 gccgtgaagt tcccggacca cggagcggtg aacgcacaga cgggctttct gctgaggaag
    17941 gtctcgacgg cctcgttcaa cgccttcgcc gagatcgtgg ccgagcacga cctgcacccg
    18001 atgcacttcg ggatgctgac gatcctcgcg gacgaggagc cgatcgcgca gcacgagctc
    18061 ggcgagaaga cgggcgtcga cccgagcacg atggtggcgc ggatggacgc cctcgaggag
    18121 cgtgggctga tcgagcggat gcggagcgcg caggaccggc gcagctacga gatccggctc
    18181 tcgccggagg gccgcaggtg cctcgcgcag ctccgccgca aggcgaaggc gcacggggac
    18241 cggttcttcg ccgcgctcag cgagagcgag cgagcccagc tgcacaggct gctggcgaag
    18301 ctggcggcga gcctcgacta gccggattca gtcgaggcgg tcgggcaggc cgaagctgct
    18361 gaaggtgctc tggagccgcc tgggatcggc cgcctcctcg ggccagcgcg agtagtcgtt
    18421 gtcgtcgccc tcgatcgtgc gcgccacgaa cgccgtcacg ttgctcacct cgcggccgcg
    18481 cagggtcagc acgttcagcg cgaacggcac gtgtgcctgc gcgctctcgt cccaggcgta
    18541 gaagccgatg gccggctggc cgttcgcgcg cgtgacgacg gtccgccagc gccagaggcc
    18601 gttcatcgac cacctggcgg cccaggccgc gatcccctcg cggccctggt accagctcgc
    18661 gagcggcggc atggcgaagg tcgcgtcctc ggcgagcatg ccggcgaacg tctcgacgtc
    18721 gtcgcgctcc caggcgtcca cgtagcgggc cacgagctcg cgcagctcgt cgtcgccgag
    18781 gttgcggagc gtctgctgct ggctctggtc cggcacccgc tcgtccaccg cggcacgggc
    18841 gcgctggagg gcgctgttca ccgacgcgac gctcgtctcg agcgcctccg ccacttcctt
    18901 cgcggagaac ccgagcacct cgcgaaggat caggacggcg cgctggttcg gcgggaggtg
    18961 ctggagggcg gcgacgaacg cgagctcgat gctctcgcgc tgctcgtagc gggcatcggg
    19021 cccggcgagg ccgtcctcga ggccgagcat ctcgtcgggg tagggctcga tccagacggt
    19081 ttccacaatc ggctcgccgg gcgccgtgtg cgggtccgag gccggcgcgt agtcgaccgg
    19141 gagaacgcgc ttgggccgct tggcgatcgc gttcaggcag gtgttggtgg cgatcgtgta
    19201 gagccaggag cgcagggcgc tgcggccctc gaagcgcgcg agcgcgcgcc aggcgcgcag
    19261 gagcgcttcc tggagggcgt cctcggcgtc gtgcgtcgag ccgagcatcc ggtagcagtg
    19321 ggcgtggagc tcgccgcggt agggctcgac gaggcggccg aacgcgtcct ggtcgcccgc
    19381 gctcgcctgc tcgaggagcc gctgctcgtc tgcgcttgtg gatgaagtcg gcatgtcggt
    19441 tggaccgtcc gccggtaccg aagtcatcgg accgatgagt ttcggcggcg ggagcggtct
    19501 tagtggggat gatggcagcc acctccaggc cggtcgtcca tctcgagctg cacactcgcg
    19561 acctcgcggg cgcgcaggcc ttctacggcg agctgttcgg gtggaagccg gagcgggtcg
    19621 agacgcccca cgggagctac gtggcactcg ggctcggggg tgagctcggc gggggcatgg
    19681 tccagtgcca gacaccgcgc tcgctgtggc tgccgtatgt ggaggtcggc gacatcgagg
    19741 aggcgacggg ccgcgcgcag gacctcggcg ccaccgtcct gctcgacccg cgcgagggtc
    19801 ccgccggctg gcgcagcgtg gtggtcacgc cggccggcgg cgagatcgcg ttctggcagc
    19861 agaagcgctg agcggcaccg tcgcacggca gccgtcgcgt taaatgacgc ccgcatgggc
    19921 gtcgagggct cagggctgac ggccggcggg gggcgcgagc gcttcctcgc gggcgacccg
    19981 atccgcggtg tggccgcggt gctcgtggtc gtctaccaca ccgctttcct ggcccagccg
    20041 ccgcagggcg agtcgctggc ggacctcggg ctggtctacg gcgcggccgc cggcgacgcg
    20101 ctgcgcgcgc tcgacctgac ggtgttcgtc ttcctcgtcc tgtccgggta cctgatcggc
    20161 cgcccgttcg tgcactcgtt cgtcgccggc cggccgatgc cgccggtcgg ggcgtacgtg
    20221 cgcaaccgcg tcctgcgcat cgtcccgacg ttctgggtcg tgttcggggt tgtcgtgctc
    20281 ctgcacggat tgtcgaacta cccggccggc gacgtgctgt cggtcttcgc gttcgcgcag
    20341 aactaccacc cgagcgaggt ggccgtcctg attggcccgg gctggacggt ggacgtggag
    20401 gtgaccttct acctggcgct gccgctcgtc gcgctggtgc tgatcgggct cggtcgcacg
    20461 cgcggcacgc ccggcgcgcg ccgcgcggtg ctgctcgggt tgctcgcgat ggtgtatctc
    20521 gccagcctga tggcgaagga cttcgatccc cccggcggga cgctgacgct gatcacggtc
    20581 ggcttcggct tcatgccggg gatcgcgctg gcggcgctcg agacgactcc gtgggcgcgc
    20641 acgctcgccc tgcgcgccgc gcgcaactgg agcctcgggc tggtggcggt cgctgtcgtg
    20701 gtactcgtcc cgtacgcgat ctcgggggat cccgggcact acccgccgcg gccggagacg
    20761 gccgcccgcg tgatggcgtc tgtgttcgcg tgtgcggtcg tggccgcgcc gctcgtcctc
    20821 cagtgggggg gtggcgggtg cccgtcagcg gtggacaacc gggtgacgcg ctggctcggg
    20881 gcgcgctcgt acccgatcta cctcgtgcac caggggatcc tgctcgagtt cgtgggctgg
    20941 tccgtgctct ccgagcgcgc gtggccgcac ctggcgctcg tgctcgcgat cgcgatgccg
    21001 ctggtgctcg cggcgggcgc gctgctgcac gttgccgtcg agcggccggc gctgcggcgg
    21061 cggcgcgact ggcgcgcggc gcacgagctg cgcgggcggc tgggacgggg gcgggcagcc
    21121 ccccgaatcg gggattgacg ccgcctgggc ggcggacgag ggttcgattc ggcatccgtt
    21181 ttcgactcag gggggcgaca tggcgcagga gatcgagggg ctgttcgagc ccagcaggta
    21241 cgagctgcag gcgaaggacc tcgagatcac ttacacgcag ctggtcgggg gagttcccga
    21301 cgtgacgatc aaggagggcg gcgtcgagaa gcgctaccac ggcacccagg tgcgggtgga
    21361 tcgcgagacg gggctcggtc gcatcgtcac ggtcatgacc gacttcatgt tcgacggcaa
    21421 ccagacgacg ctcagcctcc tcctcccgag cgtgaacttc ggcgacaagg cggaggtccc
    21481 ggtgcggacg gtggcgatcc agaccgtgca caaggggagc atcggcggcc cacagctgat
    21541 cgacggccag ctgtcgacgt acaagtcgat ctcgctgcgg gggaaggcgc ggaggattcg
    21601 ggggtaggcg ccgggggcgg tcggggttat cgcgtggcag ccgggtactc cggctcccga
    21661 gggcgggagc ccccacgagg gagcgacgag atgaccgctg tggggcgtcc gccgctgctc
    21721 aggcgggtga tctctcgacg ctgggtgtca acgcggacat gctcgagccg agccgacgcc
    21781 gactatgcgc gcatggaaac tggcgccgtc ggcgacggct ccgacgtcaa cggaacgaga
    21841 ctagagagaa ccccgcccgg gttagggggc ggcggttcgc gcgtccaatc aaatgattga
    21901 gagatcggca cgacgtgcga gtctgcctga tcagggccct ggcggtgttg ctggagccga
    21961 cgtccaataa cgccgcctga gccatagagt caattggacg gggcgtaagc catacctaca
    22021 gccggctgcg gttggtggcc tcgcggggtt tcagcccggg tccttgcgcc gctccaaagc
    22081 gatccacgtc cacgcaaagg tgatgccgaa gaagatcgcg cctccataga ggctcaaata
    22141 gatgagcagc gcgtgatggg tgccggtcga gcttagactc aggccctgct gtaagaacag
    22201 cggggcgagg gccgccgagg ccgcgcggac cagcctgagg gctacgtccg atgtgagggg
    22261 agtcgccgcc ctcacccggc gctgtggcgc ggcctccgcc aaggcatcgc gttttgcacg
    22321 acctctgtct tcactcgaac ccatctcgct ccttatcgca ggccgcacgc agacgttgcg
    22381 cctgcgacaa gtctgaacca gacgtcccct acgcgaagga tccggtgagc gtgctcagga
    22441 aaacgtgttg agcgcgctca acacgttgga ggggcgcgcc gtgcgtggct taggctgcca
    22501 ccatgatcga attcgtttcg ggtggacggt cgtgattctt acgtccctac ccgctgccga
    22561 gcaggttggt gtcgcgttct cgggcggtct ggacacgtcg gtcgcagttg catggatccg
    22621 cgcgaacgga ggcactcctt acgcgtacac cgccaacctt gggcaatacg acgagcctga
    22681 tatcgcgagc gtgcccgagc gagcattggc gttgggcgcc gaggctgcgc ggctagttga
    22741 ctgtcgagcc gaactcgtcg aagagggtct gctcgctctg gcgtgcggtg catggcacgg
    22801 atcgacggcc ggcaagacgt atttcaacac cactccactc ggacgcgcag tcacaggtac
    22861 acgattgctg attgaaatgc agaccgacgg cgtggacgtt tggagcgacg gctcgaccta
    22921 caagggcaac gacatcgagc gcttctatcg ctatgggctg ttgatgaacc cattgctgcg
    22981 catttacaag ccgtggctcg acgacaggtt cgtgtctgaa ttcggggggc gggccgagat
    23041 gagccgttgg ctcgaggagc gtgggctgcc gtgccgacac agcggtgaaa gcgcgtactc
    23101 gacagacgcg aacatctggg gggcgacgca cgaagcgaag cggctcgaac tgctcgacga
    23161 ggggatggac ctcatcgacc cgattatggg cgtggctcac tggcgaggca acgtggagat
    23221 cgaggaagag acggcggtcg ttgagttcga gtgcgggtgg ccagttgcga tcaacggctg
    23281 ccgcttcggc tcagcgatcg aggtcgtcac cgaggccaac gccatcggcg gccgccacgg
    23341 gctagggatg agcgaccaaa tcgagaaccg catcatcgag gcgaagagcc gtgggattta
    23401 cgaggcaccg ggcatggccc tgctgcatat cgcgtacgag cgcctggtta cggcaattca
    23461 caacgaagac acgatcgatc agtaccgctc gatggggcgt aggctcggtc gtctgctcta
    23521 cgaggggcgc tggtacgacc cgcaggcgct gatgcttcgc gactcgcttc agcgcttcgt
    23581 cggggcaccg gtcaccggcg aagtcgcagt ccgtctgcgc cgcggcgacg actacacact
    23641 cgagcgaacc ggtggaccgg gtcttaccta ccgcccggag cgcctgtcaa tggagcgctc
    23701 cgagggcgcc gccttcaagg ctgccgaccg ggtgggacaa ctgaagatgc gcaacttgga
    23761 cattgcggac tcccgcgcga agctcaaatc gctcggcggg tcagtgttgg ccaacccgca
    23821 cacccagcgc ctgtcaagcg cttggcctcc aaacgcaccc gaactgagga ccaaagatgg
    23881 ctgaagagac actggcgagc ttactgaagc gcttcctagc ggatcgcggc ttcagagagc
    23941 tctcgagcta tcgcgtaatg agcaggcgag gtgggctcga tgaccaagcg atcgagcgaa
    24001 tcttgagcgg taagacgaag cgcccgagcc gagcgaaact cctgagaatc gtcgagacac
    24061 tgcgactcga acaacacgag cgcaacgcac tcgaggccgc gtcgcaccac tccgagacgc
    24121 acccgggctt cctcacagtt acccgggccg agcgtgtccc ggcgccgggc caggaaccgg
    24181 cacttggccc cggcagggtc cgggagggcc ccatgtggat gcagcgtggc gtcatactcc
    24241 tgacggcgtt cggagatccc tccgacctga tcaaggagta cacgaagaag agcgggccgt
    24301 ggggaaacgt cgcgtggcga tccggcgtcg tgtttggccc ctatgacgtc gtggtccggg
    24361 ccacgacccc gaaggggaaa agcgtcatgg acttcgccga acaattgttt gaggacggtg
    24421 aggacccgga cgagcgctgg atggtgcgca cgatcgagac gattcctctg cgcgacgaca
    24481 tgcctttcta cctggatccg gcgccaagcc acgaccacct cacgccgaac tgcgtatggg
    24541 cgaccatctt tatccaggcc ctgggtggcg agcggcaacc cgagttcccg cagctcttct
    24601 acgagcgtgc caaccaaccc gacttctggg gtggagtgca cttcctcacg ggcgcggtca
    24661 caattggtca gtacgactct gtcgtggaga tcatcgccgg caacgtcaac atactcaagg
    24721 agtacgttcg cgcggcgcag aagcacgcgg cgacgaagcc ctacgaccgg catgcgcaca
    24781 cgatcaccta ctacacgcac cagccggacc ttcgacaggg ggcgactccg gcgaccttct
    24841 gagcgacgtt tcgaacccga agcggagggg gtgggattcg aacccacggt ccgcctgacg
    24901 acggacaccg gttttcaaga ccggcgcatt caaccgctct gccacccctc cgtagcgcga
    24961 gcgtagcccg ccggcaagcc ctccgcgcgg cccgcgccag gcgccgggcg ccctacttat
    25021 cctggtagtc ccgatccagc gcgtcggtgt acgtacgccc gtcgtcctcg gtccacgtga
    25081 tcggaagccc ggacttgccg ttgtcgtcgc ggatgcggcg gttgtaggcg atgttcgcct
    25141 cgaccgcgcc cgtgatctcg aaggtcgcgc cccagtcgag gctcgtcttc cggccgtcgc
    25201 ggaacgcggc tatcgagtag ttcccctggc cgccgtcgtt cgagaagtgc acccggccgt
    25261 gccgcttgtc gacgcggaag tcgatgaccg agcggttctt gacgtcgtac gacagcgccg
    25321 agaagctcca cgacacgccg ccttggtcga acgccaggcc gacgttcgga gactcggtgc
    25381 gcttcgtctt gtagcgcagc cgctcgccac gcgccccgac cgtcagcgtg tcgacgtcgc
    25441 ctggcgtgac ggatatgtcc tccacggcac tgaagtaacc gggcccgatg acccgcacgt
    25501 cgcccttgct gcgcttcttc agccggcgcc cgtcgatggt gatcgtgagg ccgccgcccg
    25561 agacagggat ccggtactcg ggctcgggct tgtcctgcca cggctcggcg gtcacgggct
    25621 tgatcacctt cgcgccgggg atctcgttca ccagcttgtc gccgacccag ccgacgcgcc
    25681 tcttgtaacg gtcctggatc aggaggttcg ggtggttcgt cgggctcccg tcgagcgtga
    25741 cgcggttgaa ctgcgtcttc gagccctcgc agaagggaca cggctgcacc ccgatgcctg
    25801 gggtcgcggg gctgatgtcc atcgtcttgg tcttcgcatc gccgtcccac acgtcgggct
    25861 cgaactccgg gttgccggcg gagaggtcgt agtgccactt gtcggcgacc gtgtcgacgt
    25921 agaagggaga cgtcctgccg ggccagttgt tgtcgtagac gaggatcacg tactggccgt
    25981 tgcccttcga ctcgaccgcg tacggggtga cctcgtggcc cccgccgtcg cgcccgaaga
    26041 agcccagccc ccagccctcg cggccgccgt cgcggagctt ctcgatcagc tgcgcgagca
    26101 cctccttcgg cgtggccgca accgtcctgt cgacgaccga gtcgagcgtc tgccacgccc
    26161 acgtgtaggc gatgttgcgc tgaagcatcg ggttcagccg catcccgtac gtgggtcccg
    26221 tcggcatccc cacctgccgc gtgcccggga gcgtgtcctt gtacaggagg aggctcatca
    26281 tcgcgaagcc gtagcagtgg ccaccctgca tcgcctcgtt gacgtcgttc atccactccc
    26341 gcccgacctg cgtgagggtg cacctgccgt cgaccacccg gatgcaggcg cggcttccga
    26401 acagggcccg catctcgttg gggccgaggt ccctctggct ctcgccgtag ttctcgaacg
    26461 agaagccgtc ctcggtcggc cggaacccgg agtcggcgac tatctcgccg gtcggcacgt
    26521 agtcggcgtt ctgcgcgaac gccggcggca ccgccaggag cagcagcgcg aggacgagcg
    26581 cgaacacgta cccacgtgcg gcgggctgaa ggtgcgaggc caggtggcca acgtaaccgc
    26641 gcgccgtcga cgagcgcaaa cgggttggcg ccgcggcagg cgcgcgctag ccgacgggcc
    26701 cgatcgtctt cggcgcgccg aacgcgtgca gtgcctccgg cagccggatc gtgccgtcgt
    26761 cctgctggcc gttctcggcc agcgcgatga tcgtgcgctg caccccgatc gcggtcccgt
    26821 tcagcgtgtg cacgtgccgc ggcggcgcgc cctcggcggt ccggtagcgg gcgtcgagcc
    26881 tgcgcgcctg gtagtcggtc gtgttcgagc acgacgtcag ctcgcggtag cgctcctgac
    26941 cggggaacca gccctcgatg tcgtacttct tcgcggccga cgccccgagg tcccaggcgg
    27001 cgatgttcac cacgcggtag gggatctcga gcgcctggta gatctcctcc tcgatcgcga
    27061 gcagccgctc gtgctcgtcc tcggactcct ccggctcgac gaacgcgaac atctcgacct
    27121 tgtcgaactg gtgcacgcgg aagatcccgc gcgtgtcctt gcccgcggcg ccggcctcgc
    27181 gtcggaagca gggggagaag ccggcgtagc ggcgcgggag cgcgccgggt tcgaggatct
    27241 gaccggcgtg gagcgacgcg agcgcgacct cggaggtgcc caccaggaac aggtcgtcgt
    27301 cgggcacccg gtagatctgg tcctcggtgt cgggcaggaa gcccgtgccg aacagcgcct
    27361 cctcgcggac gagcacgggc gggatgaccg gctcgaagcc cttcgcggtc agcacgtcga
    27421 ggacccagcg caccagcgcc agctcgagca tcacgagcgg cccgcgcagg tatgagaacc
    27481 gcgagccgga caggcgcgcg cccgcctcca tgtcgaccat ccagccgagc agctcgaggt
    27541 ggtcgcgccc ggcggcgccg gcctcgcccc actcgcgcac cacgtcgtcc tcgggcggag
    27601 cggtggggtc ggcgaggttc ggcagggtcg ccagggtcgc gtcgaggcgc tcctcgactg
    27661 cccccacctc cgcgcccatc tccttgatcc gccccgccac cccgcgcatc gcctcgatcg
    27721 cgccggcagc atcctccccc gagcgcttgg ccgcggctat cgcctcgctg gcccgcttct
    27781 gctcgccgcg catcccctcg agctcgggca gcagctcgcg ccggcgggcg tccagcgcca
    27841 gcacgtcgtc cacgatcccg cccggcacgt ggcggcgctc gagcgcctct cgcacgacgt
    27901 cgggctcctc ccgtatgcgc cttaggtcga gcacgacagc gcggcgcccc gggccgctac
    27961 tcgccgccct gcgaagtctc ttcctgcgcc tcgtccggcg tcggggcctt gcggccggcg
    28021 tactgctcga cgaaccgcgc cacgtcctcc gcgtccttcc cgaccacgat gttggcgggc
    28081 atgatcgcgc ccgagaagcc gccgttgcgg atcgcgaaca gcacgtcgtc gcggttctcc
    28141 ttgcggacgt tgaagttcgg gccgttcgtg cgctcgccgc cggcaacctc gtcctcgggc
    28201 ttcgacccgc gggccttcgc ctcgtcgagg tgatggcagc cggagcagcg ctccgagaag
    28261 agctcggcgc cgtggcgcag ggtggtctgg ccctcgggga cgccgatgtc ctgcttaccg
    28321 ccgcagcccg cggcgagggc cgccagcgcg gccgcggcgc aggcaagcaa gggccggggg
    28381 agcgacatcc gggcgcatat tatcggcgtg gtgcgccgtg cccctcttgc catagccgcg
    28441 atcgcgttcg ccctcgccgg ctgcggtgac gacgagtccg gctcgaaggg aagcctgaca
    28501 ctgaactccg gtcagccggt gccggtcaag gcctacgagt acgggttcga gccagggacg
    28561 atcacgattc gcgccaagaa caccgaggtc gccgtccagt tcaggctgac gaacgttgga
    28621 acgctgcccc acgacatcca cgtgcgcaag ggcgacgagg agctcggcgg ggccgaggcg
    28681 atcggagacg gcaagtcgac gagcgccacc gtgtcggtgc cccccggcga gtacgagttc
    28741 tactgctcga tcggcgacca cgccgacctc ggcatgaagg gaaagctgac ggtcgagtag
    28801 aaggccccga cgccgggctg tatcgccgcc gatgtaggcg atctgcctca aagcgtcttg
    28861 ccgtggctga ccgcccaagc cggcgccggg gcgtgtgggg aattcgcgcc acgcggcgcg
    28921 gttcctccta gaagatcccg gcggcgcgca gcgagagcgt gacgatcatc agcgccccgg
    28981 tcgtcatcac gcccatctcg cgcagaacga cacgccgcgc cagcagcacc tgctcgcgcc
    29041 gctcgagcgc ctccacgtcc tcaacctcgg ccacctcggc ctgcgcggcg cccacccgca
    29101 tgccctcggt cacgagggag acgaacagcg gcgtgaggcc gtagaacagg tgcagcccgt
    29161 cgacgtgctc gccgctcgcg aggagcacgc cgcccagcgc ggcctggatc accaccgacg
    29221 cctgcgccgc ccgcagcacg taccagaacc cggtcgaggg atcgcgccgc gaccaggcga
    29281 tcgcgcccca cgcggcggcg agcgcgttca gcgccagcac cgcaaacccc acgaccacgt
    29341 gaacctcagt cagaaagcgc cctttcgcac tatgtggcaa gttgtcacaa gcgggtatat
    29401 aaccccgctc cgcgatgctg aagagctacc tcccagcact gatcttcgtc ctgctcggtg
    29461 ccgggctcgg cggtctcttc acgtacctga acggcgtgct cggccctcgc cggccgagca
    29521 aggtcaagtc gcagccgtac gagtgcggcc tgccgtcgga ggtcacgtcg agcttccgct
    29581 tcgggatcag cttctatctg atcgcgatgc tgttcatcct gttcgacatc gaggtcgtct
    29641 tcctctaccc ggtcgcggtg gggctcaggg acttcggaat gttcgcgctg gtcgaggtga
    29701 tcgtcttcgt ggcgctgctg ttcgcggcgc tcgcgttcgt gtggcggaga ggagcgctcg
    29761 aatggaagtg acgcgccaga agctcggcgg cgacccctcc gaggacttcc ggatcaagca
    29821 gctccgcgcg cgccagatgc tgcgcggcga gatctccgac gacgacctcg acacctacat
    29881 cgaggagcgg gtcgggctca ccacgctcga gaaggcgcag gcatgggcgc gcgccaacgc
    29941 gccgtggccg ctcacgttcg ggctggcctg ctgcgccatc gagatgatct cggtggtcgg
    30001 ctcgccgcgc atggacgcgg cgcggttcgg cgccgaggtg ttccgcgcct cgccgcgcca
    30061 ggcggacatg atcatcctgt ccgggcgcgt ctcgatcaag atggcgccgg tcatccgccg
    30121 catctacgac cagatgctcg agccgaagtg ggtgatcgcg atgggcgtgt gctcgtcgag
    30181 cgccgggatg ttcaacaact acgccgtcgt ccagggcgcg gacaagttcc tccccgtgga
    30241 catctacatc ccgggctgcc ccccgcggcc cgaggcgctc atgtacggga tcatcaagct
    30301 ccagagccag atcaagggcc agcccgacct cggctggcgc gagcgctacc aggcgcgagg
    30361 gaccgaggag aaggggctcg cctacgagcg cacggaggcc gacgagcgca gccgcgatac
    30421 ggggcggccc cttgcctgac gccactggcc tggagctgat cgcgggcgtc gtccgcgacc
    30481 ggctgggcga ggacgccgtg gtcggcacga cgctctaccg cgagcaggcc acgctcgagg
    30541 tggccccgcc ggccgtgcac gacgtgctcg cgctgctgcg cgacgacgag gacgaggcgt
    30601 tcccgcacct gatgagcctg cacggctgcg actacctgcc cgaggagccg cgcctcggcg
    30661 tgcactacca gctcctctcg cgcgagcggc tcgaccgcct cggcgtgaag gtgcgcgttg
    30721 gcactgacga tccggtcgtc cccagcgtcg tcgacctctt ccccggcgcc gacttccagg
    30781 agcgcgaggt cttcgacttc ttcggcgtgc acttcgacgg ccacccggac ctgcgccgca
    30841 tcctcatgcc cgaggactac gaggggcatc cgcagcggcg cgacttcccg atcggcggcg
    30901 agccggtgct gttcaccttc aacgagcacg acatgccgcg ctggtatgac tgacgaccgc
    30961 tcggccgttc ccacgcagga cccctcgaag gccccctcgg ttgtcgacga cggaccgctg
    31021 tcggtgccct accgcgaccg cgagcggtcg gtctcgctcg agcgcatgga caagccgggc
    31081 gcgccggtgt tcggcaccga gctcgagccc gagaccgagc tcttcacgat caacatgggc
    31141 ccgcaccacc cggccaccca cggggtgctg cgcctgctgc tcacgctcga gggcgaggtc
    31201 gtgcgcgagt gcacgccgat catcggctac gtccacaccg gcatcgagaa gagctgcgag
    31261 gaccaggcct actggaaggt catcccgtac gtcgagcgga tggactacct ctcgtacaac
    31321 ttcaacgcca cagccttctg catggcggtc gagcggctgc tggaagagga ggtcccgccc
    31381 cgcgctcagt acctgcgcgt gatcatgctc gagctcaacc ggatcgcgag ccatctgttc
    31441 tggctcggca cctcggcgct cgacgtcggc gcgatctcga tgctctggta ctcgctgcgc
    31501 gagcgcgacc tgatcctcga cctgttcgag atgatcgccg ggcagcgcct gcacctgcgc
    31561 tacatccagg tcggcggcgt gatcgacgac ccgcccccgg gctgggatcg caaggtgcgc
    31621 gacttctgca ccacgatgcc aacgcggctc gaccagtacg aggcgatcct cgaccgcaac
    31681 gagatcgtgc tccagcggct caagcacacc ggcgtggtgc cggtcgagac gatgcgtggg
    31741 ctgggcgtga ccggcccgct gctgcgcgcc tgcggcgacc cgtgggacct gcgcaaggcg
    31801 atgccgtaca gctcctacga ccacttcgac ttcaagatcc ccgtgggcac gaccggggac
    31861 aactacgacc gctaccgcgt gcggatggcg gagatgcgcg agtcggtgaa gatcatcgag
    31921 caggccctcg acggtctgcc cgaggggccg tacatcacga ccaaccgcaa agtcgccctg
    31981 ccgccccgcc acgagctcgc gacctcgatg gaggcgctga tccaccactt caagctcgtg
    32041 accgagggct tccgcgtgcc gccgggcgag gtgtacatgc cgatcgagtc gccacgcggc
    32101 gagctcgggt gcttcgtgct cgccgacggc tccgcgaagc ccgcgcgcgt gcacatgcgc
    32161 gacccgtcgt tcgtcaacct ccagtcgctg ccgcacatgg cgaaggacgc gctgatcgcc
    32221 gacctgatcg cctcggtcgc gatgctcgac ccggtgctcg gggggatcga caggtgagcc
    32281 cggagagcca ggactcgcac gagatggacc cgcgcgtgcc cgagggtctg ccggtgccgg
    32341 acgacttcgg ccgcgagagc gcgatgggat cgagagcgca ggacctcccc cgcggttggg
    32401 accgctccta cgacaaggac aaggacccgg ccgtcatccc ggacatcgcc gaggtggacg
    32461 tgcccgccga cctgcgcgcc gagatcgagg cgcacatggc gaagtacccg gaccgccgct
    32521 cggcgtcgat cccgtcgctg cacgccgcgc agcgcgtgca cggctggtgc tcgccgctcg
    32581 cgatccgcca ggtggccgcg gtgatgcgcg tgacgcccgg ctacctctcc tcggtcgcga
    32641 gcttctacga catgctcaac gaggatccgg tcgggcgcca ccgggtctac gtgtgcacga
    32701 gcgtggcctg ccacgtcgtg aatgccaagg cggtgtacga cgcgatcgag tccgcgggcg
    32761 ccgacctgcc ggacaccgag atccgccagt tcgagtgcct gggcgcgtgc gacatggccc
    32821 cgatggcgtc ggtggacggc cgctacatcg ggccgctcga cccgagcgac gcgcccgaga
    32881 tcgtggcggc gatcagggag gggcgcaagc cgctcccggg ccgcggcctc gaggacgagg
    32941 actaccggct gccgtgggga gggcgggcct gatggagttc gacagcctcc ggctgatccg
    33001 caacatcaac gaccccgagc tgcgcacgat cgacggctac gagcggggcg gcggctaccg
    33061 ggcgatccgc aaggcgctgc tcgagctcga gcccgagcag gtgctccacg agctcgagga
    33121 gtcgggtctg cgcggccgcg gcggcgccgg cttcgcgatg ggcaagaagg ccagcttcat
    33181 ccccaagggc acgatggaca agtacctctg ctgcaacgcg gacgagtccg agcccggcac
    33241 gttcaaggac cgcctgctga tgcagaagaa cccgcacctg ctgatcgagg ggatcgtgat
    33301 cgcggcggtc gcggccggcg ccaacaaggc gttcatctac atccgcggcg agtacgagct
    33361 ccaggcggac acgctcgacc gcgccgtggc cgaggcatac gagcgcggct acctcggcga
    33421 gcgcatcctc gactcggacc actccatcga cctggtggtc caccgcggtg ccggcgccta
    33481 catctgcggt gaggagaccg cgctgctcga ctcgctcgag ggcaagcgcg gcaacccgcg
    33541 cctgaagccg ccgttcccgg cgaaccaggg cctctaccag gggccgacgc tgatcaacaa
    33601 cgtcgagacc ctctgcaacg tgccggcgat catcgagaac ggcgccgact ggttcaagtc
    33661 ggtcggcacc gagaagtcgg cgggcacgaa gctcgtgtcg gtctccggca acgtgcgccg
    33721 gccgggcaac tacgagatcc cgctcggcat gcgcgcggcc gacatcgtct acgggctcgc
    33781 gggcggaccg ccggaggggc gcaggatcaa gtgctggttc cccggcggct cgtcggcgcc
    33841 ggtgctgctc gaggaggacc tcgacctcga ctacgacttc gagacgatgg ccgcggccgg
    33901 gtcgatgctc ggctcgggcg cgatcatcgt ggtggacgac tcgcagccga tcgtcgggct
    33961 cgcactgcgc ctcgccgagt tctaccgcca cgagtcgtgc gggaagtgca cgccgtgccg
    34021 ggagggcacg aactggaccg tgaagatgct cgagcggatc gacaccggcg aggccacgcc
    34081 cgcggacctg gacgtgatgg cggaggtcca gaccaacatc atgggcaact gcctctgcgt
    34141 gctcggcgac tcgatggcga tgccgatcgg ctcgatgatc gagaagttcc gcgatgagtt
    34201 cgagcgccac atcgaggagg cgcggctgcg caacaacggg ctgccgctgg cggcgcacgg
    34261 aggacgcgcc gatctggcgg tcgggagccc ggccgcctag ccatgcctcg cccggagccc
    34321 aagttcgtca cgttcgaggt cgacggccgc gaggtctcgg cgcccgaggg cgcgatgctc
    34381 gtggacggcg ccaagtacgg cgacgtcgag atcccggtct tctgttacga gcccaagctc
    34441 ggcgcgccgg tcggcgcctg ccgcatgtgc ctcgtcgaga tcgagggcat ccccaagctc
    34501 cagaccgcct gctcgacccc ggtcaaggac ggcatggtgg tgaccacgac ctccgaccgg
    34561 gtcaagcacg cgcagaacgc ggtcgtggag ttcctgctcg tgaaccaccc gctcgactgc
    34621 ccggtctgcg acaagggcgg cgagtgcccg ctccaggaca tcacgttcgg ctggggcgcc
    34681 gggcgctcgc gcttcatcga accgaagcgc cacttcaaga agccgctcga gctctcgccg
    34741 ctggtccaca tcgaccgcga gcgctgcatc ctctgctacc gctgcgtgcg cttctcgcag
    34801 gagatctccg aggactacca gctcgtgctg gtggagcgcg gcgaccacac cttcgtgggc
    34861 acgcacgacg gccacccgta cgtcggcccg ttcagcggca acatcaccca gctctgcccg
    34921 gtcggcgcgc tgctgccctc tacctaccgc ttccgcgcgc gcccgtggga catcgagaac
    34981 gcgggcacgg tctgcacgat gtgcccgagc cagtgcaacg tcgagctgac ggtgcgcgac
    35041 gacaagaagg tgctgcgcgt gaacgcgcgc gacaaccacg aggtggacga cggctggctg
    35101 tgcgacaagg gccgctacgg gttcggcgtg ttcaacgcgc cggagcggat cgtggcgccg
    35161 atggtgcgcg acggcggcga gctgcgcgag gtctcgtggg agcgcgcgat cggcgatgcc
    35221 gtgaaggcgc tcgaccgggc cggcgagaag accgcggcga tcgtgggcgg cgaggccacc
    35281 aacgaggagg gcttcctcct tcagttcctc atgcgcgagg tgctcggctc ctcgcacgtg
    35341 gactcgcgca tcggcggcac gctcgacccc gagcaggccc gccagctcgc gcggcccgac
    35401 ctggccgcgc aggtctcgga cctcgactac gcaggcgcgg tgctggtgct cggcaccgag
    35461 ctcgtggacg aggccccgat cctcgacctg cgcgtgcgca aggcggtgcg ccgccgcggc
    35521 gtgccgctga tcgtggcgac gtcgcggccg agcacgctcg acccgaacgc cgacgtggtg
    35581 ctgcgctacg cgccgggcgc cgaggaggcg ctggtgggtg cgctggcaca ggcgctcggg
    35641 gggagcggct ccgaatccgg atccggcgcg cagccgcagc gcagcgaggg ctgggacaag
    35701 gtccgcttcg cgcccgagcc gggccagccg caggagccgc agcagcagga gccggagccg
    35761 gcggtgccca ccgacttcgc cgagcgcgcc ggcgcctcgc gcgatgccat cgcccgggcc
    35821 gccggcaagc tgcgcggcaa cggcccggtc gtggtgctgt ggggcgagcg gctgagcggc
    35881 ggcgagcgcg gcgcgcaggc cacgcgcgcg ctgcttgcac tcacccgcgc gctcgacatc
    35941 ggatcgagcg agggcgccgg actgatcgag atccccgccg ggacgaacgg gcgcggcctg
    36001 cgcgaggtcg gctgcgtgcc gaacatgaag gccggcctgg ccgacatcga gcccgccggc
    36061 cacgacgccg ccgcgatcgc gaaggcgctc ggcgacgagg tgtccacgct cgtcctgctc
    36121 caggccgatc cgctgcgtac gcacccgaac cgccccgact gggagcgcgg cctcgacgcc
    36181 gccaacaccg tgatcgcgtt cagcgacttc gccgacgaga cgatccagaa ccacgccgac
    36241 atcgtgttcc ccgccgaggg ctacgcggag aaggagggca cggtcacgca ccccgacggg
    36301 cgcctccagc gcgtgcgcgc gtcggtggcc aagcccgagc tcgtgcgctc gcagaccgac
    36361 gtgctgctcg agctgctctc cgctctgtcg ggcgctccgt tccggctcag cccgccgcag
    36421 ctcacgagct tcatggccga cacggtcccg ttctaccgcg ggctgaccgt tgacgagatc
    36481 ggcggccgcg gcgtgcgctg gcaggagcgc gacgccgccg cgcagctccc gcagacgccg
    36541 ctgcccgaga ccgacctcga ggccccgccg gagctgcccg gcgggctgcg gctcggcacc
    36601 gtcccgtccc tctgggccag ctcggagacc gagcacgcgc gcgcgctccg cttcctcgcc
    36661 cccactcagc gcgccgagct ctcgcctgac gacgcgcgca acctcggcgt ggagccgggc
    36721 gaccgcgtcg aggtggccgt gaacgggacg agcgtgggcg ccacggccgc cctgcgctcc
    36781 ggcgtcccgg ccggcagcgt gttcctcatc gaggggacga ccgaggacaa cgcgaccgcg
    36841 ctcgccaacg gcaagccgcg cgtggtggag gtgcgcaagg catgacgcag ctcgccgccg
    36901 tcggcttcga ggagtcggtg ctcgtccaga tcgtgaaggc gctcctgatc ttcctgttcg
    36961 tgctccaggc ggtgccgctg atcctgctgc tcgagcgcaa ggccctcggc cgcttccagg
    37021 cgcggatcgg gcccaaccgc gtgggtccct acggcctgat gcagccgctc gccgacgtgc
    37081 tcaagctgct ctcgaaggag cagttcacgc cggccaccgc cgtcccgtgg atgatggcca
    37141 tcgcacccgc gatctcgatc ttcaccgcgg tggtcacgct cgcgatcatt ccgttcggcg
    37201 acacgcgcga gggcggcctc ttcggcgccg actacggcct ctacgggatc gacgtatcga
    37261 tcgggatcct gtgggcgttc gccttcggcg cgctcgcctt ctacggcctg atgctcggcg
    37321 gctgggcgtc cggctcgaag tactcggtcc tcggcgccat gcgctcggcc gcgcagctca
    37381 tctcgtacga ggtgtcgctg gcgctggcgc tgctcggcgt ggtgatcacg gccgggtcgc
    37441 tgtcgctcac cgagatcgtc cacgcgcagg acggcatctg gtacatcgtg ccgcagttcg
    37501 tcggcttcct gatcttcatg gtcgcgggct ttgccgagac caaccgcccg ccgttcgacc
    37561 tgccggaggc cgacgccgag atcgtcgccg gctacaacac cgagttcggc gggatgcgct
    37621 tcggctcgtt cttcatggcc gagtacatca acatgatcgc gatctccggg atcgcgaccg
    37681 cgatgttcct cggcggctgg cacgggccgg gaccaggctt cctcgacccg gtctgggtgc
    37741 tcgcgaagat gttcttcttc atctggatct tcatctggat ccgcgcgacg ctgccgcgcc
    37801 tgcgctacga ccagctcatg gcgttcggct ggaagatcct gctgccgctg gcgacgctca
    37861 acgcactggt gaccgcgatc gtggtggtgg cgacggaata gatgcccgcc tcccgacccc
    37921 cgcacccccg gatgccgctg caaaccttca ccggaccttc gtcctcggac tcgtccatgc
    37981 actcgtgcat ggacgtcgtc ctgcgtcctc ggccggctcg gttttcgcgc ccccggtggc
    38041 acgcgggcca ggaggcgaac atctcgtcat gagcccctgg aacccagacg aggacgtgat
    38101 catcgtccag cgcggccccg agcccggcgg cgtcggcggc gcctaccggg cgttcggcga
    38161 gacgctgcgc ggcctcaaga cgacgatggg ccggctgctc gagggcccgg tcacgcgcga
    38221 gtacccggag gacaagacgc cggtctaccc gcgctttcgc ggccgccaca agctgcatcg
    38281 gttcgaggac agcgacctcg agaagtgcgt cggctgctcg ctctgcgcgg cggcgtgccc
    38341 ggccgagtgc atccgcgtgg tcgccgccga gaacacgccc gacaaccagg tctcggccgg
    38401 cgagcgctac gccgtggtct acgagatcaa catgagccgc tgcatcttct gcggctactg
    38461 cgagatcgcg tgcccgttcg acgccatcac gatgggcaac gactacgagc tggccgacta
    38521 cacgcgctcg gatctgatct tcaccaagga gatgctgctc gccgacccga tcgagcgggc
    38581 gccgctgcgg cgggagggcg agtagggcat tggaggagct cctcttcttc gtcgcggcgc
    38641 tcggcgccat cggcggcgcg ctcggcgtcg tgctgctgcg caacccgttc ttcagcgtgc
    38701 tcgcgctcgt cgcgcacctg gtcgcgctgg cgctgctgtt cctgctgctg aacgcggagt
    38761 tcctggccgc ctcacagctc gtggtctacg cgggcgcggt gatggtgctc tacgtgttcg
    38821 tggtggccta catcggcggt gtggacgacc ccggcctcga ccagggcggc gccgtcgggc
    38881 ggctcgggcc gcccttcgca gtggccctgc tggtcgagct gtgcatcgcg atcgtcggct
    38941 cgggcctcgc ggcgctcgac accgcggggg ccgacgtggg cgccggcttc ggctcccccg
    39001 gcgaggtggg caagctgctt ctcgagaagt tcctcgtgcc gttcgaggcg gcgtcgttcc
    39061 tgctgctcgt cgcggcggtc ggcgcgatcg tgctggcgcg ccgcaggcgc ggcctcgtgg
    39121 acgacgccga ggatctgccg gagcgcgccg gcaaggcgcc gatcggcggc cgcgacggag
    39181 gcgtgcgcgg gatggaccag tacgacgagc agccgcccgc cggcaccgcc gagggggccg
    39241 gcgacccgac cccggcgcgc ggcgacgacc gcggcggcac gcccgaagag gtcccggcct
    39301 agatggacat cgcctggttc gtagtcctgt cggcgttcat cttctgcatc ggcgccgccg
    39361 gggtgctcac gcgtcgtaac ccgctcgtcg tgctgctctg cctcgagctg atgctgaacg
    39421 ccggcaacct cgcgctgatc tcgttctcgc ggatgctcgg cgaccacgag ggccaggtgt
    39481 tcgcgctgat cgtgatggtc gtggcggcgt gcgaggtcgt gatcggcctc ggcctgatcg
    39541 tcgcgatgta ccggaacaag atcccgatcg acgtcgacga ggtaagggag ttgcgcgggt
    39601 gagcgccggc ggctggggtt ggctcatcct gctgttcccg ctctggggaa cgatcctgat
    39661 ctcgctgttc tggcggctgc tgccgcggcg caccgcgggc tggctcggca gcgcggcgat
    39721 cctcgggtcg ttcgtcgcct cgctgggcgc gttcttctcg atccagtcgg acgacgtgca
    39781 ccaccgggtc gtggagtcga acgcgttcga ctacgtgaag accgccggcg tgaacgtgga
    39841 cctggggatc ct
//
LOCUS       DASVPL010000211         7482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_141085, whole genome shotgun sequence.
ACCESSION   DASVPL010000211 DASVPL010000000
VERSION     DASVPL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141085"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VF517_11290"
     CDS             <1..1461
                     /locus_tag="VF517_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX8053566.1"
                     /translation="GWPALVVRATLGGAPLPIVRNDLPSPSVRLCLFDGVPDEVSLAE
                     PYQGLTFGLEDGVVGPRNVTATSVAGAQIANAAPLTPTFRTPPANATGGVLDVAPLAA
                     ALETAVGITQFSSKAVVNWNGTALQTAFVSASRLDATVPANLIASAGTAEVTVTDGGA
                     TSYPARFTIDPPLAIDGLEPSMVAAGGPAFTLVVDGVGFGADSAVQWNGTALKTTVVS
                     ALEVSAAVPANLIAAAGSVSVVVATGGKSTAPAQLAIVAGGPAISELEPSIAAAGSGG
                     FTLTVVGSGFEPTGTVVNWNGAPLATRFLSPTEVSATVPASLIATPATATVTVTAGGT
                     ASSGKPFTIGGAGPQVGSFEQAVAIAGGKGFNLVVRGINFGNDAVVKWNGTALTTTWD
                     DAKQVTAAVPASVIAAAGAAAVTVESGGHASPPASFVVIAPEPGIGLLEPSTAVAGGP
                     QFKLSVTGGFGAGDFAIQMVDAPELQSFVPTHQEPS"
     gene            1458..5939
                     /locus_tag="VF517_11295"
     CDS             1458..5939
                     /locus_tag="VF517_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX8053567.1"
                     /translation="MTDASPLVVPMVVEAFVVNDWVREGNPFVRAQMQYGVLQRAGNA
                     QPGLNDRDLNFGRTNPVPPHNVAASDYYNGVYLKWRLPKAFTRGKQDSAASRMHFRCI
                     PNRWIVVRYGGPLTARVASAWIVESDYIWPTNEPQPAMTAAQVSSLYVQGAAGTAPVA
                     TYMGRNVPLGTWKEPGTSQKLTAIAPGNTAFAFYQPRNNNVLSFIDVLGSAPAETLSY
                     QVFGWFSLPGDDPLADVAKDGFETVLGELGWTLPKDTDPALTASWSLLSGYVGGVAWQ
                     TADEPPGGTPKGSPVSIAAGNTAVEALTAMVTAQAAEQGKQVDAELLEAFQLDMLDVL
                     DEPDGAARLHEKLHASFFKRFSGGYTWDVVDVPGADPVSEQELAKELAWLALLNQAQA
                     TLDADIRLLTSLQVELYEMWWKYTSWPSAYKGQTVVKGPTGAALKRDDLLNQLQPTVA
                     GTIAQRTYAQLQKVAAGQGAVPSGATEDALKASIQSYASKQGLPASRQLKRSAAPSFY
                     EPNNPVVLIAGAGASGIVPDPEATVCRFPAQLVRGFKWQGNDITASTQGLTVPRPDIS
                     GVSGAPWSSALATALVDEAFFTDPQSATAISAALPNSTVAQVQAAMASDPLGTPPAGA
                     VQAWTANPWHPLLLLWQVTYYPIAYGTPAAPNWTFEDGRYVWNGAGVQQTTAFQGVIQ
                     LSPAATFNLAARLEAFLATNPHLDPLELEELNTLLDFVKTQDNDWDVLSQALDGFNEQ
                     ARLGMSGVFMGPEASPVTTTPPLATLIGDQDSYPPGLGNVPTRVIPPSNFQPWRAGQF
                     SFAELIVIDEWGQAVWPIDDSSAKHETVYLPPDMSPVLKSNSVDFTIAAGAHVDGVAP
                     GLIAAGGPDATITVTGSGFAANAAVHWNGTALATTFQSAAQLSAVVPAAQTASAAGAQ
                     VTVQSGGAASNAVTVTVAGGPAIGSLSPNLFQAGMATSAEVALGVSGVGFTPTAVVQW
                     NGTALRTAFTSPTRLTAAVPANFLAAPGAAGVTVVDRSTTSPAATFTVAAGAAIGSIA
                     PASALAGSQALTLTVTGIGFEPGAAVTWKGAALVTTFVSPTQLTASVPAANLATADTA
                     AVCEQLGHTVIPAATETFAQLPPALLEPGRLDFDMLSATDDSVVFGPSAPAADPVCGW
                     VLPNHLDGSLMAYDSAGTALGELSLGVGASGPAHPCWTAAPGSPYRTLAQIGAAIKHF
                     GPFLETLGGQDAATFAAFLAAIDETLWTTVPMGAAFDKSLAVLIGRPLALVRSRVQFA
                     LDGLAYPDPSWQYTFTPQPNLLAGYELAIELGNVAQLNDGLIGYFVEDSYGRFNIVQQ
                     AGAVASTYLHPIGVDDNYVYLPFDGTTQTHLSMLVDPRAPVHATTSILPDVTLDLPAR
                     FVDDALAAMDVTFRLNGVLTDQQIATPAPANGDGDGGDDDDDDDDGETTTVQLPLPDE
                     KVGTWTWVEKDVGGWVSYPTAVNDATARLTEVPPVLRRGLLQLSAALGKGAKEEKRS"
     gene            5936..>7482
                     /locus_tag="VF517_11300"
     CDS             5936..>7482
                     /locus_tag="VF517_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053568.1"
                     /translation="MTVISRPNSWVTVAGDSETTLTYAPRVDPAPLTVSVPGRDATLG
                     SLVVVITNGTADDLDVASVTFTIVVGAPGKEGSPLMPTTKGVKTVVSDTTTWAFNGPD
                     APVTSGTADFVLAPVTGSTAKLAAGTSVYVEIYDFETMQIPGTSTITVAEAIGTSDPE
                     FTSFSISTFPAGFFFDSLIPTVQSGSALVPVAQVANGSTVTLTWNSSLSDLKNQTVYY
                     SSATSGQQKVTPSVLGAWTSPPLTSDTVFAVAVVAQGIGGEPLTAALVTGVSVQNPAL
                     VASSLTAGQVTSTGAASVVGPLTANGITATGITVNGALVANGVTVNGTLSAAGATLSG
                     ALSAGATTVNGALSAASANVSGNVQAGSIATGTATVNSSLYARQSKVSMITGAQTINP
                     GNYVANTDGFVLGTVGWPSSADPGCVGYASGQSAGMTVFATGGNLGAFGPRWSDYQAS
                     NGNTFVLPVAAGNNYVINAQQVDGGLQQAAAPIWFYWVPLGTAPSGLATTERLGDAPD
                     DFVPLPKP"
BASE COUNT         1101 a         2717 c         2701 g          963 t
ORIGIN      
        1 ggctggccgg cgctggtggt gcgcgcgacg ctcgggggcg cgccgctgcc gatcgtgcgc
       61 aacgacctgc cgtcgccgtc ggtccggctg tgcctgttcg acggggtgcc ggacgaggtg
      121 agcctggcgg agccgtacca ggggctgacc ttcggcctcg aggacggcgt cgtcgggccg
      181 cgcaacgtga ccgccaccag cgtcgcgggc gcgcagatcg cgaacgccgc gccgctcacg
      241 ccgaccttca ggacgccgcc cgcgaacgcc accggcggcg tgctcgacgt cgcgccgctc
      301 gcggccgcgc tagagaccgc ggtggggatc acgcagttca gctcgaaggc cgtcgtcaac
      361 tggaacggca cggccctcca gaccgccttc gtgagcgcga gccgcctaga cgccaccgtg
      421 ccggcgaacc tgatcgcgtc ggcggggacg gccgaggtca ccgtgacgga cggcggcgcg
      481 acgtcgtacc cggcgcggtt caccatcgat cccccgctgg cgatcgacgg gctcgagccg
      541 agcatggtcg cggccggcgg cccggcgttc acgctcgtgg tcgacggcgt cggcttcggg
      601 gccgactccg ccgtccagtg gaacgggacg gcgctgaaga cgacggtggt gagcgcgctg
      661 gaggtgagcg cggccgtgcc cgcgaacctg atcgccgccg ccggcagcgt gagcgtcgtc
      721 gtcgcgaccg gcgggaagtc gacggcgccg gcgcagctcg cgatcgtcgc gggcggcccc
      781 gcgatcagcg aactcgagcc gagcatcgcg gcggccggca gcggcgggtt cacgctcacc
      841 gtcgtcggat ccgggttcga gcccaccggc acggtcgtga actggaacgg ggcgccgctg
      901 gcgacgcgct tcctcagccc gaccgaggtg agcgcgaccg tgcccgcgag cctgatcgcg
      961 acccccgcga cggcaaccgt gaccgtgacc gccggaggga ccgcctcgag cggcaagccc
     1021 ttcaccatcg gcggcgcggg gccgcaggtc gggtcgttcg agcaggccgt cgcgatcgcc
     1081 ggcggcaagg ggttcaacct ggtcgtgcgc gggatcaact tcggcaacga cgcggtcgtg
     1141 aagtggaacg gcacagcgct cacgacgacc tgggacgacg cgaagcaggt aaccgccgcg
     1201 gtaccggcga gcgtgatcgc cgcggccggc gcggccgcgg tcacggtcga gtcgggcggc
     1261 cacgcgtcgc cgccggcgag cttcgtcgtg atcgcgcccg agccgggcat cgggctgctc
     1321 gagccgagca cggccgtcgc cggcgggccg cagttcaagc tcagcgtgac cggcggcttc
     1381 ggcgccggcg acttcgcgat ccagatggtg gacgcgcccg agctccagtc cttcgtcccg
     1441 acccaccagg agccgtcatg acggatgcga gcccgctcgt cgtaccgatg gtcgtcgagg
     1501 cgttcgtagt caacgactgg gtccgcgagg gcaacccgtt cgtgcgcgcg cagatgcagt
     1561 acggcgtgct ccagcgcgcc ggcaacgcgc agcccgggct gaacgaccgc gacctgaact
     1621 tcggcaggac caacccggtc ccaccccaca acgtggcggc ctcggactac tacaacggcg
     1681 tctacctgaa gtggcggctg ccgaaggcgt tcacccgcgg caagcaggac tcggccgcgt
     1741 cgcggatgca cttccgctgc atcccgaacc gctggatcgt ggtgcgctac ggcggtccgc
     1801 tgaccgcccg cgtggcgagc gcctggatcg tcgagagcga ctacatctgg ccgacgaacg
     1861 agccgcagcc ggcgatgacc gccgcgcagg tcagctcgct ctacgtgcag ggggcggccg
     1921 ggacggcgcc ggtcgccacg tacatgggcc gcaacgtccc gctcggcacc tggaaggagc
     1981 ccggcacgtc gcagaagctc accgcgatcg cccccggcaa cacggcgttc gccttctacc
     2041 agccgcgcaa caacaacgtg ctctcgttca tcgacgtgct cggcagcgcg ccggccgaga
     2101 cgctcagcta ccaggtgttc ggctggttct cgctgcccgg cgacgacccg ctcgccgacg
     2161 tggccaagga cgggttcgag acggtcctgg gtgagctggg ctggacgctc ccgaaggaca
     2221 ccgaccccgc gctcaccgcg agctggtcgc tgctgtcggg ctacgtcggc ggcgtggcct
     2281 ggcagacggc cgacgagccg cccggcggca cgccgaaggg ctcgccggtc tcgatcgcgg
     2341 ccggcaacac cgcggtcgag gcgctgaccg cgatggtgac cgcgcaggcg gccgagcagg
     2401 gcaagcaggt cgacgccgaa ctgctcgagg cgttccagct cgacatgctc gacgtgctcg
     2461 acgagccgga cggcgcggcg cggctccacg agaagctgca cgcgagcttc ttcaagcgct
     2521 tctcgggtgg ctacacctgg gacgtcgtcg acgtgccggg ggcggacccg gtgtccgagc
     2581 aggagctcgc caaggagctc gcgtggctgg cgctgctcaa ccaggcccag gcgacgctcg
     2641 acgccgacat ccgcctgctg acctcgctcc aggtcgagct ctacgagatg tggtggaagt
     2701 acacctcgtg gccgtcggcg tacaagggcc agacggtcgt gaaggggccg accggcgcgg
     2761 cgctcaagcg cgacgacctc ctgaaccagc tccagccgac ggtcgcggga acgatcgccc
     2821 agcggacgta cgcgcagctc cagaaggtcg cggccggcca gggcgcggtg ccgagcgggg
     2881 ccacggagga cgcgctgaag gcgtcgatcc agagctacgc gtcgaagcag ggcctcccgg
     2941 cttcgcgtca gctcaagcgc tccgcggcgc cgtccttcta cgagccgaac aaccccgtgg
     3001 tgctgatcgc gggcgccggc gcgagcggca tcgtgccgga cccggaggcg accgtctgcc
     3061 gcttcccggc gcagctcgtg cgcggcttca agtggcaggg gaacgacatc acggcgtcga
     3121 cccagggcct gacggtcccg cggcccgaca tctctggcgt gtcgggcgcc ccctggtcga
     3181 gcgccctggc cacggcactc gtcgacgagg cgttcttcac cgacccgcag agcgcgaccg
     3241 cgatctccgc cgcgctcccc aacagcacgg tcgcgcaggt ccaggcggcg atggcgagcg
     3301 acccgctcgg gacgccgccg gccggcgcgg tgcaggcgtg gaccgcgaac ccctggcacc
     3361 cgctgctgct gctctggcag gtgacctact acccgatcgc gtacgggacg ccggccgcgc
     3421 cgaactggac cttcgaggac gggcgctacg tgtggaacgg cgccggggtg cagcagacga
     3481 ccgcgtttca gggggtgatc cagctgagcc cggccgcgac gttcaacctc gccgcgcggc
     3541 tcgaggcgtt cctcgccacc aacccgcacc tcgacccgct cgagctcgag gagctgaaca
     3601 cgctgctcga cttcgtgaag acgcaggaca acgactggga cgtgctgtcg caggcgctcg
     3661 acggcttcaa cgagcaggcc aggctgggca tgtcgggcgt gttcatgggc ccggaggcga
     3721 gcccggtcac gaccacgccg ccgctcgcga cgctcatcgg cgaccaggac agctacccgc
     3781 ccggcctcgg caacgtcccg acgcgggtga tcccgccgag caacttccag ccgtggcggg
     3841 cggggcagtt ctcgttcgcc gagctgatcg tgatcgacga gtggggccag gcggtctggc
     3901 cgatcgacga cagcagcgcg aagcacgaga cggtctacct gccgccggac atgagtcccg
     3961 tgctcaagtc gaactcggtc gacttcacga tcgcggcggg ggcgcacgtc gacggcgtgg
     4021 cgcccggcct gatcgcggcc gggggccccg acgcgaccat cacggtgacg ggctcgggct
     4081 tcgccgccaa cgcggccgtg cactggaacg gcaccgcgct ggcgaccacc ttccagagcg
     4141 cggcgcagct ctcggcggtc gtcccggccg cgcagaccgc gagcgccgcc ggcgcgcagg
     4201 tgacggtgca gtcgggcggg gccgcctcga acgcggtgac cgtcacggtc gcgggcgggc
     4261 cggccatcgg ctcgctcagc ccgaacctct tccaggccgg catggccacc tcggccgagg
     4321 tggcgctcgg cgtgagcggg gtcgggttca cgccgaccgc ggtcgtgcag tggaacggca
     4381 ccgcgctgag gaccgcgttc acgagcccga ccaggctgac ggctgccgtg ccggccaact
     4441 tcctggccgc accgggcgcc gcgggcgtga ccgtggtgga ccgctcgacc acctcgccgg
     4501 cggccacctt caccgttgcg gcgggagccg cgatcgggtc gatcgcgccg gcctcggccc
     4561 tggccgggag ccaggcactc acgctgaccg tcacgggcat cggcttcgag cccggcgctg
     4621 ccgtgacgtg gaagggggcg gcgctcgtga cgacgttcgt gagccccacg cagctcactg
     4681 cctcggtgcc ggcggcgaac ctcgcgaccg cggacaccgc cgccgtctgc gagcagctcg
     4741 gtcacacggt gatcccggcg gccaccgaga cgttcgccca gctccccccg gcgctgctcg
     4801 agccggggcg gctcgacttc gacatgctct cggcgaccga cgacagcgtc gtgttcggtc
     4861 cctcggcccc ggcggccgac ccggtatgcg gctgggtgct ccccaaccac ctcgacggct
     4921 cgctgatggc ctacgactcg gccgggaccg ccctcggcga gctctcgctg ggcgtgggcg
     4981 cgagcgggcc cgcgcacccg tgctggacgg ccgcccccgg cagcccgtac cggacgctcg
     5041 cccagatcgg agccgcgatc aagcacttcg gaccgttcct ggagacgctc ggcggccagg
     5101 acgcggccac gttcgcggcg ttcctcgcgg cgatcgacga aacgctctgg acgaccgtcc
     5161 cgatgggcgc ggccttcgac aagagcctcg cggtgctgat cgggcgcccg ctggcgctgg
     5221 tgcgctcgcg cgtgcagttc gcgctcgacg ggctcgccta ccccgaccca agctggcagt
     5281 acacgttcac gccgcagccg aacctgctcg cgggctacga gctcgcgatc gagctcggca
     5341 acgtcgcgca gctaaacgac gggctgatcg gctacttcgt ggaggactcg tacggtcggt
     5401 tcaacatcgt ccagcaggcg ggcgccgtgg cgagcacgta cctgcacccg atcggcgtgg
     5461 acgacaacta cgtgtacctg ccgttcgacg gcacgacgca gacgcacctg tcgatgctgg
     5521 tcgacccgcg cgcgccggtg cacgcgacga cctcgatcct gcccgacgtc acgctggacc
     5581 tcccggcccg gttcgtggac gacgcgctgg cggcgatgga cgtgacgttc cggctcaacg
     5641 gggtgctcac cgaccagcag atcgcgacgc cggcgccggc gaacggcgac ggcgacggcg
     5701 gcgacgacga cgacgacgac gacgacggcg agaccacgac cgtccagctc ccgctgcccg
     5761 acgagaaggt ggggacctgg acgtgggtcg agaaggacgt cggcggctgg gtcagctacc
     5821 cgacagccgt gaacgacgcg acggcgcggc tgacggaggt gccgccggtc ctgcggcgcg
     5881 ggctgctcca gctctcggcc gcgctcggca agggagcgaa ggaggagaag aggtcatgac
     5941 cgttatcagc aggccgaact cgtgggtcac cgtcgccggc gattccgaga cgacgctgac
     6001 gtacgccccg cgggtcgatc ccgcgccgct gacggtcagc gtgcccggcc gcgacgcgac
     6061 gctcggctcc ctcgtcgtgg tgatcacgaa cggcacggcc gacgacctgg acgtcgcctc
     6121 ggtcacgttc acgatcgtcg tcggggcgcc cggcaaggag ggcagccccc tcatgcccac
     6181 gacgaagggc gtgaagacgg tggtctcgga caccaccacg tgggcgttca acgggccgga
     6241 cgctccggtc acgagcggga ccgcggactt cgtgctcgcg cccgtcaccg ggagcaccgc
     6301 gaagctcgcc gccgggacgt ccgtgtacgt ggagatctac gacttcgaga cgatgcagat
     6361 accgggcacg agcacgatca cggtggcgga ggcgatcggc acgagcgatc ccgagttcac
     6421 ctcgttctcg atcagcacgt tccccgccgg gttcttcttc gacagcctga tccccaccgt
     6481 gcagagcggc agcgcgctgg tcccggtcgc ccaggtggcg aacgggtcga ccgtgacgct
     6541 gacgtggaac tcctcgctat ccgacctgaa gaaccagacg gtttactact cgagcgccac
     6601 gtccggccag cagaaggtga cgccgagcgt gctcggcgcg tggacctcgc cgccgctcac
     6661 gagcgacacg gtgttcgcgg tcgccgtggt ggcccagggc atcggcggcg agccactgac
     6721 ggcggccctg gtgaccgggg tgtcggtgca gaacccggcg ctcgtggcca gctcgctgac
     6781 ggccggccag gtgacgtcca ccggcgcggc gtcggtcgtc ggtccgctga cggcgaacgg
     6841 catcacagcg accggcatca ccgtgaacgg cgcgctggtg gccaacggcg tgaccgtgaa
     6901 cggcacgctg agcgccgccg gcgcgacgct cagcggcgcg ctcagcgccg gcgccaccac
     6961 cgtcaacggc gcgctgtcgg ccgcgagcgc gaacgtgagc ggcaacgtgc aggcgggctc
     7021 gatcgccacc ggcacggcca ccgtcaactc gtcgctctac gcgcggcaga gcaaggtgtc
     7081 gatgatcacc ggcgcgcaga ccatcaaccc cggcaactac gtggccaaca ccgacgggtt
     7141 cgtgctcggc acggtcgggt ggccttcgag cgcggacccc ggctgcgtcg gctacgcgtc
     7201 cggccagtcg gctgggatga cggtgttcgc gaccggcggc aacctcggcg cgttcgggcc
     7261 gaggtggtcc gactaccagg cgagcaacgg caacacgttc gtgctgccgg tcgccgcggg
     7321 caacaactac gtgatcaacg cccagcaggt cgacggcggc ctccagcagg ccgcggcccc
     7381 gatctggttc tactgggtgc cgctcgggac cgcgccgtcc ggcctcgcca ccaccgaacg
     7441 gctcggcgac gcgccggacg acttcgtgcc gctaccgaag cc
//
LOCUS       DASVPL010000212         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_298940, whole genome shotgun sequence.
ACCESSION   DASVPL010000212 DASVPL010000000
VERSION     DASVPL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298940"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VF517_11305"
     CDS             <1..377
                     /locus_tag="VF517_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053569.1"
                     /translation="QTRRMVDATKRLLPSGALVGYRIDNVRITRPHRSKIRVRCGPCL
                     FLIVQARPGKAPRLLTRRILGSVSRKSLLRKTLRPGSRVEVFATRPMWIGRYYAFKFG
                     RKSFTRAEQCLTPPENRRAKCR"
     gene            368..778
                     /locus_tag="VF517_11310"
     CDS             368..778
                     /locus_tag="VF517_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053570.1"
                     /translation="MPVADPKRSTRWSQGARALVAGLITFGLLVLAGWGIATAKSALF
                     EEDDLLPQRLDAGAAPARPELERARTLPHLGRGPKPPHRPGAGGSPPANDGPSGEPNP
                     SPRPDPSPTGQGGGQPAPAPAPDPGLRDGDKTGR"
     gene            909..1847
                     /locus_tag="VF517_11315"
     CDS             909..1847
                     /locus_tag="VF517_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053571.1"
                     /translation="MRSARAAVAGLPQKVALGAGAAFMGTALATGWWLGDRSDGRERI
                     RTAEVELELPAGWQRVPRAPSVPGISAETMTAYAPDADAERGALMVGSQPAHGETLLP
                     EPVARRVRAWPRPIPVGLGGYDAFRYEVAPGRRRLRMYVIPSVATGRDIVIACLLPAS
                     ADVAQLMENCDRAAAGVRIDDARSYSRAAARAYAKRVRTVLEDLDDERPGLRRKLAAA
                     RTSKGQAKVAGTLADLHAEFADRARGLTYEPPLGKINGVVVRALDDAAEAYEDLAEAA
                     EERDRDRFEAERDAVAASERGLQRPLDRLAVLISPR"
     gene            complement(1862..2197)
                     /locus_tag="VF517_11320"
     CDS             complement(1862..2197)
                     /locus_tag="VF517_11320"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8053572.1"
                     /translation="MAGDLVYFVVPAADAERARAFYGALFGWEFSPGNVPGGFNIEGP
                     TPPGGLFGPSDATRPLPYFQVDDIEAAVARVRELGGEAEDPQEIASGWMSHAKDDQGL
                     EIGLWQGRG"
     gene            complement(2197..2823)
                     /locus_tag="VF517_11325"
     CDS             complement(2197..2823)
                     /locus_tag="VF517_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8053573.1"
                     /translation="MPEHKVFAAAYNAIFAGLEKGGLADKRRELLAQARGATLELGAG
                     TGHNLPHYPAAVERLVLTEPDPHMAKRLRAQLAESGRSGAEVVEAGAEALPFGDDEFD
                     TVVATLVFCTIPDPRAALDEVARVLRPGGRMLFLEHVRAHSPRRARWQDRLNPVQNLL
                     FGGCNLNRPTAATLEASPLEVEELRDDRMPRVGGPLVEPMIVGSARVA"
     gene            complement(3213..>4642)
                     /locus_tag="VF517_11330"
     CDS             complement(3213..>4642)
                     /locus_tag="VF517_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX8053574.1"
                     /translation="LIALRDIDRVIELIRGAPDPDAARDSLIAEFELSREQAQAILDL
                     RLQRLTALEAGKIEQEHADLMERIRELREILGDEQRISGLIKEELQEIRERYGDERRT
                     EITHSEDEVGIEDLIADQQMVISITKSGYIKSLPLSTYRSQRRGGVGVIGMDMKDEDY
                     IEHLFVCSTHDYLLFFTNRGKVYRQKVYDLPEASRTAKGRALVNLLPLREGEFVRAVL
                     STRDFTEDKYLVFATKKGQIKKTEFLAYNTPIKADGIIAISIRDDDELVAVRRTSGDD
                     DIIMVSRSGQAARFNATAVRSMGRDTAGVRGMNVQRGDNCVLAMDVARDDSELLVVTE
                     NGFGKRTAISEYPVKGRGAMGVKTIQITEKKGGLAGALIVREHHELVFISQNGMVQRT
                     AVRGISRYGRASQGVKLMNLREDDQVSAVALVMETTAEAGAAVAEELPGEELPGGDGA
                     VLEDGDVAAPEDTVSDNGTSPPEAE"
BASE COUNT          619 a         1670 c         1666 g          687 t
ORIGIN      
        1 gtcagacgcg ccggatggtc gacgcgacca agcggctgct gccgagcggt gccctggtgg
       61 ggtaccggat cgacaacgtg cggatcactc gtccgcacag gtcgaagatc agagtgcgct
      121 gcgggccctg cctgttcctg atcgtgcagg cgcgcccagg caaggctccc cgacttctca
      181 cgcgccggat cctggggtcc gtgagccgga agagccttct gcgcaagacg ctccgccccg
      241 ggtcgcgggt cgaggtgttc gccacgcggc cgatgtggat cgggcgctac tacgccttca
      301 agttcggaag gaagagcttc acccgggcgg agcagtgcct cacaccgccc gagaaccggc
      361 gggcgaagtg ccggtagccg acccgaagcg ctccacgcgc tggagccagg gcgctcgggc
      421 gctggtcgcg ggactgatca ccttcggcct gctcgtcctc gccggctggg ggatcgccac
      481 cgcgaagtcg gcgctgttcg aggaagacga cctcctaccg cagcgcctcg acgcgggagc
      541 ggctcccgcg cgtcctgagc tcgagcgcgc gcgcacgctg ccgcacctcg ggcgcggacc
      601 caagccgccg caccgcccgg gcgcgggcgg atcgccgccg gcgaacgacg gtccgagcgg
      661 cgagccgaac ccttcccccc gtccggaccc ctcccctacg gggcagggcg gcggccagcc
      721 ggctcccgct ccggcgcccg acccaggcct gcgcgacggt gacaaaacag gccggtgatc
      781 gccccaggcg agcacgcgcg ggcgcgcggc ttcggcgcgt aacgaggagg gccacccgcg
      841 cactggcgcg ggtcggccgg gccttggacc ggggggctcg cgccacgggc gcgatgaccg
      901 tggacggctt gcgttccgct cgcgccgccg ttgccggcct gccgcagaag gtcgccctgg
      961 gggccggggc ggcgttcatg ggcaccgcgc tcgccaccgg gtggtggctg ggcgaccgtt
     1021 cggacgggcg tgagcgcatc cgcaccgccg aggtcgagct ggagctccca gccgggtggc
     1081 agcgcgttcc gcgggcgccc tcggttccag ggatcagcgc cgagaccatg accgcgtacg
     1141 cgcccgacgc ggacgccgag cgtggcgcgc tgatggtcgg ctcgcagccc gcgcacggcg
     1201 agacgctgct gcccgagccc gtcgcgcggc gtgtgcgagc ctggccgcgg ccgatcccgg
     1261 tgggcctggg cggctacgac gcgttccgat acgaggtcgc gcccggtcga cgccgccttc
     1321 gcatgtacgt gattccatcc gtcgcgaccg gccgcgacat cgtgatcgcg tgcctcctgc
     1381 cggcctccgc ggacgtggcg cagctcatgg agaactgcga tcgcgcagcc gccggcgtcc
     1441 gcatcgacga cgcgcgctcc tactcccgcg cggcagcgcg cgcgtacgcc aagcgggtcc
     1501 gcaccgtgct cgaggacctc gacgacgagc gtccggggct gcggcgcaag ctcgcggccg
     1561 cgcgcacctc gaagggacag gcgaaggtgg cagggaccct ggcggacctg cacgccgagt
     1621 tcgccgaccg tgcgcgtggc ctgacctacg agccgccgct cggcaagatc aacggtgtcg
     1681 tcgtgcgagc gctggacgat gctgccgagg catacgagga cctcgccgag gccgccgagg
     1741 agcgcgatcg ggaccgcttc gaggcggagc gcgatgccgt cgccgcgagc gagagagggc
     1801 tacagcgtcc gctcgaccgg ctcgcggtcc tgatctcccc gcgctgattg cggactggag
     1861 ctcagccgcg gccctgccag aggccgatct cgagcccctg gtcgtccttc gcgtgcgaca
     1921 tccagccgga cgcgatctcc tgcgggtcct cggcctcgcc gccgagctcg cgcaccctgg
     1981 ccaccgcggc ctcgatgtcg tcgacctgga agtagggcag cgggcgggtg gcgtcggagg
     2041 ggccgaacag gccgccgggt ggcgtcgggc cctcgatgtt gaagccgccc ggcacgttgc
     2101 cgggcgagaa ctcccagccg aacagcgcgc cgtagaacgc cctcgcccgc tcagcgtctg
     2161 cggcggggac gacgaagtag acgaggtcgc cggccactac gccacccgcg ccgatcccac
     2221 gatcatcggc tcgaccagcg ggccgccgac gcgcggcatg cggtcgtcgc gcagctcctc
     2281 gacctcgagc ggcgaggcct cgagcgtggc ggcggtcggc cggttgagat tgcagccgcc
     2341 gaagaggagg ttctggaccg ggttgaggcg gtcctgccag cgcgcccgcc gcggcgagtg
     2401 ggcgcgcacg tgctccagga acagcatgcg gccgcccggg cgcagcacgc gcgcgacctc
     2461 gtcgagcgcg gcccgcgggt cggggatcgt gcagaacacc agcgtcgcga cgaccgtgtc
     2521 gaactcgtcg tcgccgaacg gcagggcctc ggcgccggcc tcgaccacct ccgcaccgct
     2581 gcggccggat tccgcgagct gcgcccgcag tcgcttggcc atgtgcgggt cgggctcggt
     2641 cagcacgagg cgctcgaccg cggcggggta gtgcgggagg ttgtggcccg tgccggcgcc
     2701 gagctcgagc gtcgctccgc gcgcctgcgc gagcagctcg cgccgcttgt cggcgagccc
     2761 gcccttctcg agcccggcga agatcgcgtt gtaggcggcg gcgaagacct tgtgctcggg
     2821 catccgcgcg atcctacgcc gttcgcgcgt tcgttggcag acgagaaggg gcgccccgct
     2881 aggggcgccc cttcgtccaa cactgctgaa ttatcgactg cggcactgaa gcaccttcgc
     2941 tcgcaccggt gcgcagaatc ggccttgtgc tccttcgcgc ggtggccctc gctaatgcgc
     3001 cggccggggg gaccaaatcc gacctccgcc ttcgacggta cccggggtgg actccctagg
     3061 cgggtccagc gtccgaccta gcggccgggc acctccaggg tcccgttcaa actgtcactc
     3121 aagttaggac cacctccttc ctctggtacg cccatataag cacacccggt ggctggagcg
     3181 gaaggcccga tttcggggac gccgcggcgg cgctactcgg cttctggcgg ggaggtcccg
     3241 ttgtcggaga cggtgtcctc cggagcggct acgtcgccgt cctccagcac cgctccgtcg
     3301 ccgcccggca gctcctctcc cggcagctcc tcggccaccg cggcgcccgc ctcggcggtc
     3361 gtctccatca ccagcgccac cgcactcacc tggtcgtcct cgcgcaggtt catcagcttc
     3421 acgccctgcg aggcgcgccc gtagcgggag atgccccgca cggcggtgcg ctggaccatg
     3481 ccgttctgcg agatgaagac gagctcgtgg tgctcgcgca cgatcagcgc gccggcgagg
     3541 ccgcccttct tctcggtgat ctggatcgtc ttcacaccca tcgcgccgcg gcccttgacc
     3601 gggtactccg agatcgccgt gcgcttgccg aagccgttct cggtgacgac gagcagctcg
     3661 gagtcgtcgc gcgccacgtc catcgcgagc acgcagttgt cgccgcgctg gacgttcatg
     3721 ccgcggacgc cggcggtgtc gcggcccatc gagcgcacgg cggtcgcgtt gaagcgcgcc
     3781 gcctggcccg agcgcgagac catgatgatg tcgtcgtcgc cgctggtccg tctaaccgcg
     3841 accagctcgt cgtcgtcgcg gatcgagatc gcgatgatcc cgtcggcctt gatcggcgtg
     3901 ttgtaggcga ggaactcggt cttcttgatc tggcccttct tcgtggcgaa gaccaggtac
     3961 ttgtcctcgg tgaagtcgcg cgtggagagc accgcgcgca cgaactcgcc ctcgcgcagc
     4021 ggcagcaggt tgacgagcgc gcggcccttc gccgtgcgcg aggcctcggg caggtcgtag
     4081 accttctgcc tgtacacctt gccgcggttc gtgaagaaca ggaggtagtc gtgcgtcgag
     4141 cacacgaaga ggtgctcgat gtagtcctcg tccttcatgt ccatgccgat cacgccgacg
     4201 ccgccgcggc gctgcgagcg gtacgtggac agcggcagcg acttgatgta gccggacttc
     4261 gtgatcgaga tgaccatctg ctggtcggcg atcaggtcct cgatgccgac ctcgtcctcg
     4321 gagtgcgtga tctcggtgcg ccgctcgtcg ccgtagcgct cgcggatctc ctgcagctcc
     4381 tccttgatca ggccgctgat gcgctgctcg tcgccgagga tctcgcgcag ctcgcggatc
     4441 cgctccatca ggtcggcgtg ctcctgctcg atcttgccgg cctcgagcgc ggtgaggcgc
     4501 tggaggcgaa ggtcgaggat ggcctgcgcc tgctcgcggg agagctcgaa ctccgcgatc
     4561 aggctgtcgc gggcggcgtc gggatcgggg gcgccgcgaa tcagctcgat cacgcggtcg
     4621 atgtcgcgca gggcgatgag ga
//
LOCUS       DASVPL010000213        38562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_24814, whole genome shotgun sequence.
ACCESSION   DASVPL010000213 DASVPL010000000
VERSION     DASVPL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 38562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38562
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24814"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF517_11335"
     CDS             complement(<1..234)
                     /locus_tag="VF517_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8053575.1"
                     /translation="MVGGGLAGLATARRVASAGWTVVVLEARPDRVGGRLETHTHDGF
                     PFDLGATWIGAGHDRAAALVAELGLRPRRTHADG"
     gene            complement(267..671)
                     /locus_tag="VF517_11340"
     CDS             complement(267..671)
                     /locus_tag="VF517_11340"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8053576.1"
                     /translation="MTGGCLCGGVRFELTEPPTDAGYCHCTRCQRRTGTAASPQARID
                     ARTFRLLQGEELVGGWDPGDGGFEKCFCRSCGAHLFSRSPDGREQMSIRLGAFDGDPG
                     VRPSWRSFVAYAAPWEPIPDDGLTRYDEAKPR"
     gene            complement(668..2233)
                     /locus_tag="VF517_11345"
     CDS             complement(668..2233)
                     /locus_tag="VF517_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HEX8053577.1"
                     /translation="MTIHRGPEPDVEIPDLDLTAFVLEHAGERADKPALIDGPSGRTL
                     TYGQLAQSIRALAAGLTARGFGKGDVLAVYMPNLPEYAIALHGVASVGGKCTTMNPLY
                     TASEVAHQLEDSGARFLVTVGPMLAVASEAAAKAGIEEVFVVGEGDGATPFTELMGNP
                     DEAPEVEIDPDGDLAVLPYSSGTTGLPKGVMLSHRNLVANMSQNQIAYPIAEDDRLIG
                     VLPFFHIYGMVVIMNQGLRAGATIVTMPRFDLDQFLELIQEHRITRAYVVPPIALALA
                     KHPAVDNYELGSITGIMSGAAPLGGELAQAVAKRLDCTVIQGYGLTETSPVTHCIRPS
                     GENRPGSIGQALPNTECRLVDPETGADAQAGERGELWIRGPQVMKGYLNNDEATSHTV
                     DEDGWLHTGDIGVVDEDGFFTIVDRLKELIKYKGFQVAPAELEALLINHPDVQDVAVI
                     GVPDEEAGELPKAYVVPAGDSLDSDALMQWCAEKVSPQKKIRLVEEIDAIPKSASGKI
                     LRRELKDREAAAR"
     gene            complement(2259..3578)
                     /locus_tag="VF517_11350"
     CDS             complement(2259..3578)
                     /locus_tag="VF517_11350"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8053578.1"
                     /translation="MAGLPDIPSVPVVPRIPGVPDPLRRIDVPRDLAAVTTVGPEDEP
                     AAAGMSAAAVDRIWKATTDWYRSGVHPAIQVCVRREGVVVLDRAIGHARGNGPRDGAG
                     VEKVPVTPATPFLIYSGAKAITAVVVHMLHERGLLDIHDRVCEYIDGYERHGKDDVTI
                     GHVLAHRSGVPNLPREALDVQNAHDWELIVRWLIDAKPASKPGNRLAYHAVSGGFILG
                     EVVRQVTGRDIRTVLAEEILDPLGFRWTNYGVPERDLDTVAADYLTGPRALPPLSTFM
                     TRALGLPFEELVASANTPEFRTAIVPSANIVTTANELSRFYELLRRGGELDGVRVMEP
                     TTIRQALAEQSHLEVDLSLGFPTRFSYGLMLGAQLLSLYGRDTQHAFGHLGFTNILSW
                     ADPQRATAVAVITNGKPLLYPDVPRFYNLTQRITSEAPKVAASEILV"
     gene            3601..4212
                     /locus_tag="VF517_11355"
     CDS             3601..4212
                     /locus_tag="VF517_11355"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8053579.1"
                     /translation="MAPISDALRRGEAAAGPALDRLIAAGREHFGRDGYAGTSLDAVV
                     AAAGVTKGSLYHHFKGKADLFEAVFRQRAAELSEQVAAASLPERDPWQAAYAGVRAYL
                     DGSQEAAVERIMLLDGPSVLGWERVREIEAEYGLALIKTSIGRLVDAGEIRRPDVDVL
                     AHLLFGAITEGVLLVTRSPDPSAARRTVEREIRRLVDGLGEPA"
     gene            complement(4213..4872)
                     /locus_tag="VF517_11360"
     CDS             complement(4213..4872)
                     /locus_tag="VF517_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8053580.1"
                     /translation="MTVPLKTRILLADDHALVRHGLKLIINREADLEVVAEAGDGAEA
                     VKAALAGEIDLAILDVSMPRLTGIQVARELTQQRPELRTLILSMHDSEQYFFEALRAG
                     ASGYVLKSVVDRDLVEACRATMRGEPFVYPGAVRALIREHLERSPSSDDPPTDPLSPR
                     ESEVLKLVAEGLTSREIAGTLSISEKTVERHRGHILDKLGMRDRVELTRYAIRRGLVE
                     P"
     gene            complement(4869..5825)
                     /locus_tag="VF517_11365"
     CDS             complement(4869..5825)
                     /locus_tag="VF517_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8053581.1"
                     /translation="MRPSLFWRIFLPNATVLMIAWAMLAFSPAALVSPTVAPLEFAAG
                     LAGLIVIYVVNVLVLRRELAPIGRLRALMSTADPLRPGRRIPIYGDSAEVVELTEAFN
                     EMLARLERERRTSASSALEAQESERLRLARELHDEIGQRLTALLLELENVSREVPPGV
                     AAPVGAARESARSTLEEVRRIGQRLRPEALDDLGLGSALANLCDRLAAQTGLECACRI
                     DPGLPPLESDEELAVYRVAQEAMTNAIRHGDPSRIELQLARDDGAVALRVLDDGTGIA
                     GHEEGAGLTGMRERALMIGAHLEIGAGPDGGAAVQLTLPAPP"
     gene            5918..6682
                     /locus_tag="VF517_11370"
     CDS             5918..6682
                     /locus_tag="VF517_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8053582.1"
                     /translation="MTSISDTQVRVLDADPDLAAGLPADELPAATHAALARTYLLATG
                     TWTPPASWAGSASTLGLLVLDGLVMRSLELGDRSSTELLGEGDLLRPWQRDTEEALVA
                     CSVQWRVLEPTRIAVLDGRFALAIARWPRLTAAVVGRALRRSRAHGVFAAISHVNRVD
                     DRLVLAFWYFAERWGRVGPDGVAVRLPLTHEQLGAMIGARRPSVTTALSSLAAQGILV
                     PRRRGEWVLTPTARLRIDGLPEAARGGAQHAPRLAA"
     gene            6762..8402
                     /locus_tag="VF517_11375"
     CDS             6762..8402
                     /locus_tag="VF517_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053583.1"
                     /translation="MILALAAAVPAAGAAAPPATPQLGCGPGSRPEPGMQGRVSAADV
                     ASGAADRGFTCNTTQVGHEGASGGFKVERFVDRAGHDCAYYDTTLLFPLNAQNLGDQP
                     TGVAVLDMSDPANPKRTATLATPAMQSPHESLNLSRERGLLAAVLGNPGAAPGVVDVY
                     DLNDDCRQPQLQSSAPVAFLGHESGFAPDGKTFYATSLFDGHITAVDLTNPKTPLPIT
                     VIESFSHGMNTSDDGNRGYIAARDVGLQILDLSEIQARKPLPQVREISRLRWDTMSIP
                     QNAIPITIKGKAYVVEVDEFSVESGGGSFPEGNGPQVGAARLIDISDEAHPRVVSDMR
                     LQVHQPENRGQLEGDPGASSFVQGYAGHYCNVPRRKDPEIVACTFINSGLRVFDIRDP
                     EKPKEIAYFVAPPRPSPVMGDRSNYAMSKPEFVPERDEIWYSDGNSGFYVVRLANGVW
                     RSGGASDCLRPARIGFRIHRTGRNRVVRVDAFVNGRRKLLRRGRDITRIELSKLPRKG
                     SMKVRIVATHNTGSKVVSTRTWSGCVKGRPTVRQIRRR"
     gene            complement(8441..9589)
                     /gene="dxr"
                     /locus_tag="VF517_11380"
     CDS             complement(8441..9589)
                     /gene="dxr"
                     /locus_tag="VF517_11380"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX8053584.1"
                     /translation="MKRIVLLGATGSIGRQALDVIARSPDLELAGLSARSTWEPLVDE
                     GRRHGARRIALDDPAAARDAASAWPEAEVLSGPEAAAELAASTDADLVLNAIVGAAGL
                     GATISTLESGKQLALANKESLVVGGSLVMDLARRMGARIVPVDSEHSALDQLIEAGPP
                     GAVERLVVTASGGPFRGRSPEEVAQASVEDALAHPTWSMGVKNTIDSATLMNKGLEVI
                     EAHHLFGLPYERIEVLVHPTSIVHGLVTLTDGAVLAHLGMPDMRMPISYAMHFPERVD
                     VAVEPLDLVAASPLVFEPVDDRIAPCLRIARAAAEAGGTATCVLNAANEVAVAEFVEG
                     RLRFGDIARVVEETLERMPVQPADSVGTLLEADAEARATAHDCVPARR"
     gene            9760..11463
                     /locus_tag="VF517_11385"
     CDS             9760..11463
                     /locus_tag="VF517_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8053585.1"
                     /translation="MLGWLEEPTAERGVRFARGISEWDVWTWAEMAAAAHGAAARMSE
                     MHGGRSGTVAIVIPNSPEFVAAFYGALAAGLTPCPIAPPKAIDEPQRYRRHVAGLLEA
                     AQPALVVTVAECRDALAPIALDCPLLELSADRTAPLRPLAPPPDIALLQFTSGSSGRP
                     RAVQVTRPNLEANIRAIIAKSAMDGQVDHVATWLPTYHDMGLIGCLLATAAATAGLSM
                     LRVEDFVRRPMRWLECLGAEDGAATITATPPFGFGLALKRVTDDDLAGLDFSRWRVAI
                     VGAERVDAAVLRRFLERFSENRLSPSVLCPAYGLAEGTLLVSAKQGPRAATVIRPDWS
                     TLRFGEPVEVDAVTTLADLETLGDGVDWLVSCGDCPDGIGVEIVGEDGAPLPDGTLGE
                     IAVTGSCVTAGYLNEPEVTAERFAGGTLRTGDAALRHDGELYVFGRMADALKVNGRWL
                     SIEEIEAKVLASGAVARGRSVLFSGRDPEGDAVIALSEAEAGEWVSAVADVLGAEVPD
                     TMSIHVLSGGWGTIMRTSSGKPRRRAMWEAYLADTLDAEVVYSRRANAPDEQLAGGRS
                     A"
     gene            11460..11747
                     /locus_tag="VF517_11390"
     CDS             11460..11747
                     /locus_tag="VF517_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8053586.1"
                     /translation="MNDTQAPPGHRDMMARLQDLYEGSRGPGRTLRGDDRLADDLGID
                     SLQLMEMLAILEEQLGIEIVGDPGLFNITTVDDLVELLAHRVAGDKPRSKD"
     gene            11750..12784
                     /locus_tag="VF517_11395"
     CDS             11750..12784
                     /locus_tag="VF517_11395"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8053587.1"
                     /translation="MSPEDTTVTWPASAALVGDEDRLLDGTTEVLLAPVRGRDPRREL
                     PRELFHKRPALFVLKFGFASLLIAASCAVILAGLAWPLQALAVVVLGLMYAHLVELQH
                     ETLHEHAFNSRRLNRWFGFVCGLFMFSSYSHYKYDHLRHHADLGTDNNREFFNYRFQN
                     LHRPDGMIRAMWHMGRYADVFRDIVRSIARRPLPGIRERHNKRIQDEYRLFAVAIVAA
                     CAFTAITGDFVLLLLWLLPLLAIAEPTHFLIELPEHFGLNTQSNPDVLSNTRTVAAGP
                     LLRWFTNSNNLHTAHHYHQGVPMVNVPELHGLIDHQVRAAEDSYWSFFAKVATGKLRY
                     EDMGATCMTR"
     gene            12790..13749
                     /locus_tag="VF517_11400"
     CDS             12790..13749
                     /locus_tag="VF517_11400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX8053588.1"
                     /translation="MTTTATPTTPAAHTTEAPFQGSGSPGRPSRKWMLKELHRLDPEK
                     DAGRITELSYSARFSRMQLSYHMYYSMFFLRASGAPESARPIDRDGKGFFYRNGHKRA
                     DDTTADIFGWMHHGPESELGRASIARVKRMHDNLAARWGMPNHVLVHTLACDLLSPHW
                     LMEMIGGPKFDDHERLAHLNFWRAVGDGLGITDVPETWDGMVAYVKEYEASEHFQPSP
                     HGKRLAHGFADQFCERWFPRGFRWLGRWVLLSLVEDHVLDVHGFERPPRPVVRLTREA
                     TRLTAYLAYYVLRDPSPTFSPGELFRSGHPDIAACPVKHATPS"
     gene            13749..14990
                     /locus_tag="VF517_11405"
     CDS             13749..14990
                     /locus_tag="VF517_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053589.1"
                     /translation="MALLGYPLDGSAERADRWEQSLGVMPFDPAVNMLGWEETRRRPA
                     LALRLRLALLVRRFDVALSEGLTDWRRLAPKVSAGRERIYFWGSEEAIAAARSWFSGG
                     RLPDDGTLGGLELGTTTYVMEDHGPATIAVADHAASCTTAVPAIGVGLVTCDELFREG
                     VASMRTEIRSRHPAPGVPSGMASHLLAVREFRLSYWGVAPWYIGWGEHEMLGYFELPA
                     DPERTRRMILDAPGPFIADASDRAFADALVALNLGEDRPAAADAPPGTKAQAGPIRWE
                     VHLPGPLLMPWHRAVVRPTRRRDGSTLDAALSTVAATPRNACTTVEVPVEDPAFLPVQ
                     RQLVASGFQLAALAPPVHIEGEGRLGFTGLWTRVTSELPVAAPYYLTSTLLNNRESEV
                     VSHVHRLSDRWTGHQRASRAA"
     gene            14932..16299
                     /locus_tag="VF517_11410"
     CDS             14932..16299
                     /locus_tag="VF517_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8053590.1"
                     /translation="MSTGSVTGGPDISARPGPPEDGAGRDPVAVIGAGVSGLVACGEL
                     ERRGIPYVCFEKGEGLGGVWRRYRDVVTVTCRDAMELEALPMPPGTPDFPTGDHIIRY
                     MEDYADAFELRDRIVHGVSIASLDREPDGRWRAVHSDGTSTLHRAVILATGRIGEPAW
                     PELEGSFDGRFLHSGDYRDGSEFEGKRVVVIGFGNSAVDVACDVARYASTTHLAIRTG
                     TWVVPRYFAGRPLDKASGPIVTRVPMALRWPIYRALLWAIHGNMEAYGLPAPDAKPGT
                     RPLTVSDELIHRIGAGQVTPAAAAVALRGDRVEFADGSSVEADAVICGTGYRIEVPLL
                     EDYLAGRGLTLDGLWLNVSPPEVEDLYVVGTMVAFGAIPPLAEAQAALVAELIGGAGE
                     PPPERVKRREIAQERRRRRRYSGDTAQWMVGETPAYLRRLRKARSAARRRAGAEGRAT
                     AGSRA"
     gene            16296..16775
                     /locus_tag="VF517_11415"
     CDS             16296..16775
                     /locus_tag="VF517_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053591.1"
                     /translation="MSTPPPARLEVNGLMLSGFTELGFGAMTGWIYALALEEPDRARA
                     IGIKSVDRVRQWHLDLIALGGLSVLVGTALPSLPRRVSVPLAVGCWTNAMSFGVLMVK
                     PDLTENMVYRAAITGSFAITSAGFVGAAREAWSRRRRPPAPPATISGGGNGALDGGR"
     gene            16762..18912
                     /locus_tag="VF517_11420"
     CDS             16762..18912
                     /locus_tag="VF517_11420"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8053592.1"
                     /translation="MAVVESRRRLMLVLWGIVFVVALVPAAKQADHLTAGGYEVPGSG
                     SHAVEQAAHRFPQLRPNAIGVLVDPPDGRRIAAADVTRSRAEIAPAIEKVPSLSLGAP
                     STTLLAPGETAAVIPLRGPQDENSAIDAAVELREHIAEEAGENRLYLVGQPAVWAGHD
                     EVSKESLASAEVVGFPLTLLILVAVFGSLAAAMLPLALGFVSIVVCGALIYGLAELTP
                     MSLFVSNVASLIGIAVGVDYSLFILARYRRELAAGRDQREALQITMRTSGRVVLFSGV
                     AVETALAGLFLIDSLALRSIALGAMVVVAVAVVAALTLTPAVILTMGERLHRPSARIA
                     RVRSALSGLVRRRSTPDEFWARWSDRVTRRPWLSLAAALAVLLVLAAPALRMKTVTQA
                     LPELPASSDVRTGAEAAIERFGPAASGETYVLVTYPRRVDNATNRHIGAVVAADPAVT
                     HVAPPAVSGDGRHALFSIVPRSPAETADTRRLAERLEGKLASVAARVPGVSIAVGGTA
                     AHEAQFDEQITGSLWKVVLFVLLASAAVMFVLLRSVLLPLKAIVMNVLTVGAAFGVLV
                     AVFQWGWFDGFLGFESPGAVSTLALPFVLAIMFGLSMDYEVFLLARVQEEYRRSGDTR
                     SAVQHALRTSAGAISSAAVIMIVVFLAFVAVSLATIKMIGLGLAVAVLLDATIVRLVL
                     VPAAVVLMGDANWWAPSWLKRRDRETPPMVAAGG"
     gene            complement(18909..19823)
                     /locus_tag="VF517_11425"
     CDS             complement(18909..19823)
                     /locus_tag="VF517_11425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8053593.1"
                     /translation="MRLSKVPTVPLSLLREYSTRSVEARVPEPMVMDDADSVAEYHEV
                     GKKTQAAIYQLSALATSHLAPEGAHVLDLGSGSGQNLAHLARRRPDLRITGVDLSDLM
                     IETAEAMFAEEGLDDRVKMVQGDITDLADLVPDDLGVVSSVQSLHHLPSFDHLQRCLE
                     QMAAARERTGCAVWIFDEARLRHGKSYGGFMETAFNFPLPPKLKHDALASERAAFTPA
                     EMVEYLGRAGLGDLHGSAMYVLPAFHLYWPRRRDGAPSGHERCWRAVPPLPGTAIDTF
                     MFGWMFPDVPHREGSSLAAVARRGPRSV"
     gene            19933..21171
                     /locus_tag="VF517_11430"
     CDS             19933..21171
                     /locus_tag="VF517_11430"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX8053594.1"
                     /translation="MRPLLLILAALFALPAAAQADVIPFGSDLSGAADVDQSHGEDTA
                     FWNVALANGGAVTAPAEGHITAIKVKGGVLTRTGDNKLARQIHFQVLDPTADGPKVRV
                     SSADFYLPRTNDQQVVTTYEEPELVNICVNKGDFIDLNTIGGAEFAAGGDAGSALQVF
                     SRTPGSTVNWYESGGGTNNGAVFPRQVALNNTELLLQAVLSTGPDASDFCPGGYRQHI
                     FRGLAFPAPTQPTVKTKTRRVRLKGECNGENYGGCFGELTLSAVIDGASRTLGQVDFS
                     IAPGVKNTIEIPISEASVLAIQKAKILKGTLTAVAHDNPRGDARVKWDSVPRQEKTTT
                     DEISLKPDRPACVVPGGLIGKSSASAKAKLKAAGCGNVVKFKKVTKSKQWSKVVSVKP
                     KAGTVLPAGTRVTLTIGRRK"
     gene            21197..22783
                     /locus_tag="VF517_11435"
     CDS             21197..22783
                     /locus_tag="VF517_11435"
                     /inference="COORDINATES: protein motif:HMM:NF045452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053595.1"
                     /translation="MRSLALVCAVAALLLAASGAQAQSSFDQNGVLFVHGFVGTGGQF
                     ESQKLRFTSNGYPERWVEAIDYDSTFATESRAQVHARIDALIAELKKRTGRPKVDLLG
                     HSLGTSVLQEYLGSSAERAANVAHYVNIDGSQADALPGGVPTLAIWAGRGEPGRRIVG
                     ATNVTLPNQTHVQSATSAEAFVEYHKFFTGRAPAHDIVPQAGQITIAGRVLHFPLNRG
                     VAGATLAIWEVDGATGQRTGSAPLATTSIGESGDWGPVEVTAGRHYEFEVTRPGAPVH
                     HHYYEPFVRSDHLVRLLESDALANGGERGPRHAAGVILRYKELWGDQPGQNDALSING
                     TNVCPEVVCPVSKRVNALFFSDRRMDGQSDLSQPDPVYSALPFVTGVDLFAPAAIPPT
                     GRTAVEIRSRGGGPTRAVNMPNFASLTDVASVQLNDYERIDGSGPCLRPASIPFRLHR
                     VEGTRVVRVEAFVNGRRTLVSRGRDIRRIELRKLARTGTLRVRIVATHNTGSKVVSTR
                     SWSGCTKGKPRVRVVRRSGA"
     gene            complement(22792..23691)
                     /locus_tag="VF517_11440"
     CDS             complement(22792..23691)
                     /locus_tag="VF517_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053596.1"
                     /translation="MGRWPPIHDPQALRWISLDDETWLAELEKRVRAFGSRAYDAAAL
                     EHALGYPWERPSGSYVLRGGQVSLIEDIEPAERRAAVAEHARDRYPLVAFGANGAPSR
                     LQDRFASFEDPRDRDALVLTGELHGVDVGAQASPTAFGSMPGVLFASPGTAVRAAVLW
                     LTPAQLGELTKAELGYRLGRLDRARFTMDEAGVSVDDVFAYVSRIGTLRLDGEPVALG
                     AVPARGRSARAMTQEQLLEAVGTLALGRPVGAADVVRMCFEDMAALMSKLIPLTWPTA
                     LRLPDDEWSPYPVDDGVGTRPPG"
     gene            complement(23701..23976)
                     /locus_tag="VF517_11445"
     CDS             complement(23701..23976)
                     /locus_tag="VF517_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053597.1"
                     /translation="MVRGTRALAVSSTLFVAALVGPGSASGQSQPQPPPNPCPDSFIL
                     TPVQAVPPADADTDRNGNGFVCAKINQNPHAGLVVVDDARARHLPPI"
     gene            24108..24791
                     /locus_tag="VF517_11450"
     CDS             24108..24791
                     /locus_tag="VF517_11450"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX8053598.1"
                     /translation="MSTLGIVGGIGPPSTVAYYRTIVDLHRGRCGRPPRVVIDSIDGD
                     ELVEHLAAGDRHAVTTMLVGALDQLARAGADLALLASASAHTAYDEVRSASPLPLVGI
                     VEATAAGAVGCSRVGIFGTRFTMRADLFGPALAAEGIATVAPSPQEQEAIDRVYFDEL
                     VGGRFRPESRDALRAIAVRLRDESGVDGILLAGTELPLLLPETRYDDLVYLDVARLHA
                     EAAVEAMLD"
     gene            complement(24899..25114)
                     /locus_tag="VF517_11455"
     CDS             complement(24899..25114)
                     /locus_tag="VF517_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053599.1"
                     /translation="MLMVLFVVTLLSALGIWVIAKVTMRVMDQLGLDLMPTLQWLGLA
                     EPTSPPPPRLRDLALTPPPSPGTRVAR"
     gene            complement(25198..25270)
                     /locus_tag="VF517_11460"
     tRNA            complement(25198..25270)
                     /locus_tag="VF517_11460"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25235..25237),aa:Ala,seq:cgc)
     gene            25431..27383
                     /locus_tag="VF517_11465"
     CDS             25431..27383
                     /locus_tag="VF517_11465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8053600.1"
                     /translation="MSFRSRLLLFFTIIVVIPMVAVALVLFSITEDSETGKADAEIAQ
                     GLRAAFFLYDDARDDARSLLDDVARDPELAGALSERDQKAIERRLGQLRDKHASIVAI
                     TIYDTARKRLARDGAADAVAFAVASPSVRSGERLGFVGVSVTRASDFMQQVRRMTALE
                     VRVISQGRVLASSLNERGRAVVESGDVDVAERKYRGRFAVARDPAGPNVQLGLFVEGA
                     ELRDAIGDQRLLIGAILVAFLLLALFSSIVVVRALQTQIAQFLEAARRLARGDFSRPV
                     PVEGADEFAELAGEFNAMSAQLAGKIDEVERKRSELEETIRRVGDAFATGLDREAIVN
                     LVVRTAVEACEAEIGRALPIDARRMHRAEVGEPSPELVAALEAAERSAFAVDDDLGSD
                     WLASLEGKEDIHHHRPVRAEVGGVHSLAAQLVARLGRGGAQEQVGVVSIARHGGGFDD
                     TEYELFSYLTGQAAVSIENVDLHEIVQLQALTDELTGLFNLRHFHDTLSGEIERSRRF
                     GTDVGLAMLDIDDFKKINDTFGHPQGDLVLLEVARVLRSLSRDIDEPARYGGEEMAVI
                     LPQTDLHGAELLAERMRAAIEALEIKRLDGKGTLKVTASFGVASLPVCASDKESLIAE
                     ADAALYRAKRAGKNRTERGEPVTAEG"
     gene            27414..28070
                     /locus_tag="VF517_11470"
     CDS             27414..28070
                     /locus_tag="VF517_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053601.1"
                     /translation="MGALDDAIREHLELKRRHGATEEEIARKEAEALGPARRGPAAEP
                     AMEPAPGSPEDLPPEAVDPDGLVEPEAVELAEPDAAEPEPVEPQEPVAPLAETEPVGA
                     DEEPLATPVEPTIDVPSEPEPPAALDPAPADPIEADLPPVHDVDRDPLEPPPPVTKAA
                     PGATVEFDTLSARRDDDDLADPTDEDVLEETPEFLRETPEHDRLWFEQKPPRDFDLDN
                     "
     gene            complement(28242..29639)
                     /locus_tag="VF517_11475"
     CDS             complement(28242..29639)
                     /locus_tag="VF517_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX8053602.1"
                     /translation="MSEETTTSTEQEARESDDAPETKEQLHESSDGDGSDKSPRAANE
                     AETTTRAEISNYEIVSHYFDDAAERLGIGDDIATVLKTSYREVQVQIPIRRADGKVHV
                     YSGFRVQHNGARGPYKGGVRFHPEVDLDEVRALASLMTWKTAIAGIPYGGAKGGVNCP
                     ARELEQDELEKVARSFISKIGKLLGPQRDIPAPDVGTDAQAMAWMMDEYGKIYGHTPA
                     CVTGKPIALEGSYGREAATGRGVVHMYEEAAKELDLKPEDTRFVVQGYGNVGSWAARI
                     LQDLGAKLVAASDVEGAIRSDEGLDAHALAEHVKEGGKVCEFDGGEAISAEELIETEC
                     EVFIPAALGGMIHKHNADKLNCKLIVEGANSPTTPTADDMLADKGVMVVPDLMANAGG
                     VIVSYFEWVQNLQHFRWEEDEVNERLAKIMRRAYGEVSERAKEKDLALRPAAYELGIE
                     RVFEAATTRGYVHAD"
     gene            29729..31576
                     /locus_tag="VF517_11480"
     CDS             29729..31576
                     /locus_tag="VF517_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8053603.1"
                     /translation="MRGGQRGVIAALAAAALVPAVVAAPAHGAAIAYQADAAHSGFVP
                     DGPNPPLGRKWVRRDLGVQVSYPVIAEGKVFVTAYAADASNSSYGSSSPTGPTFLHAL
                     DRGTGATVWVRQVATGGLPAYGDGRVYVLGREALDAVSASNGERLWRAEMRDTGGAAP
                     VADGPFVYASDARGAHAVSSDTGLTVQSESLPNSGFTVVGDRVYVTQSGCKTIALYSR
                     GLAQQVWSRAGPCSSSAPVPAAYHTLNTTPPNERLWARDSNGQTGLVVDALTSLGTEQ
                     FNSEGALALAGELAFLRNPAGIQARNQASGVIAWTASPATQLRGSALAVRDAVWTTTG
                     SGELVALRRSDGGELFRTVLLAHQLTTSDHDSSASPPHSGMAADANGLVVPYVNRLVA
                     LGPGSDTPGIDELDKIPAGQTQLSAAMKPRELEFGAKVKVTGQLKSSDSQAQPHQLEL
                     QADAYPYDGVWQAVDSAATQPTYDYSLDHRPDRNTRYRVVDISTSPAVVSKTFQAWVY
                     PKLRWRFGRSRVGAVRIAATIAAPDHLALNGKPLYIYRGRTRRGTYTRIGSLKIKRRK
                     PTSYSAVGTRRVPSHRPRTDYFFVCFRIPDTNVLTRAKGRPDQCGRRRL"
     gene            31607..31678
                     /locus_tag="VF517_11485"
     tRNA            31607..31678
                     /locus_tag="VF517_11485"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31640..31642,aa:Ala,seq:tgc)
     gene            31835..32068
                     /locus_tag="VF517_11490"
     CDS             31835..32068
                     /locus_tag="VF517_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053604.1"
                     /translation="MSNRQIAVRLPDEVVKFVDAIVSSGRERSRAAVVTRALQRERRR
                     VMAERDAAILSGGEPDPELIALAEHIARLDRDL"
     gene            32092..32367
                     /locus_tag="VF517_11495"
     CDS             32092..32367
                     /locus_tag="VF517_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX8053605.1"
                     /translation="MDKVRPVLVLTREVVRPHMNTVTVAPITTTIRGLSTEVEVGPAN
                     GLEHRSVVACDHVTTIPNAALSSQIGVLLDGQEQALSDAIRTALDLD"
     gene            complement(33103..33603)
                     /locus_tag="VF517_11500"
     CDS             complement(33103..33603)
                     /locus_tag="VF517_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053606.1"
                     /translation="MTGFGRLLEDLNRAGVRYVIVGGIAVIRHGVVRATKDLDVVVAC
                     DEDTAKRLADLRVEWEATRPDGSPEERRLPSAGWPLHLRTSHGLIDLLAEQAPPLDLS
                     GLLSRADTRRVESVPAAICSLADLVALKRQGGRATDLEDIRRLETAHGELPEPPKDPG
                     VSGGAT"
     gene            complement(33600..33818)
                     /locus_tag="VF517_11505"
     CDS             complement(33600..33818)
                     /locus_tag="VF517_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053607.1"
                     /translation="MTRAERDTRLADLAADARAAERRGDGWAAGEVWRQYELVRDGGR
                     DPDELLAEGMALSQVALELAELAERSEV"
     gene            complement(33859..34128)
                     /locus_tag="VF517_11510"
     CDS             complement(33859..34128)
                     /locus_tag="VF517_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8053608.1"
                     /translation="MRLTRSPVVELNRAVAVAEADGPAAGLALVDAIEGLDGYRYLHA
                     TRADLLRRLGRRDEARAAYERALALATDDAERRFLEGRLRELAGY"
     gene            complement(34119..34997)
                     /locus_tag="VF517_11515"
     CDS             complement(34119..34997)
                     /locus_tag="VF517_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8053609.1"
                     /translation="MLATLIGLLGDFELAEDAAQEAFATAAARWPREGVPRNPRAWLV
                     ATARNRAIDRIRRERTLAEKMRLLDVPSRTAEDPMDEDTAIPDERLELVFTCCHPALA
                     TEAQVALTLRTLGGLTTEEIARAFLVPDATMAQRLVRAKRKIRHAGIPFRVPPDHLLP
                     DRLAAVLAVVYLIFNEGYGGRHDLASEAIRLGRALAELMPDEPEVHALLALMLLHDAR
                     RAARFRDGELVLLGDQDRSLWDGGRVAEGRMLLERALALRGSGPYVVQAAIASLHADE
                     QATGTRLRRSTGSSCG"
     gene            complement(35030..35368)
                     /locus_tag="VF517_11520"
     CDS             complement(35030..35368)
                     /locus_tag="VF517_11520"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8053610.1"
                     /translation="MKYMMLIWSNSTDWDQLSEEEQNAALAEYGAIGELPGKVSGEQL
                     RPGSTATTVRVRDGETLTTDGPFVETKEQLGGYYLFEADDLDAAIEIAARIPAARQSG
                     AVEVRPVVER"
     gene            complement(35400..35615)
                     /locus_tag="VF517_11525"
     CDS             complement(35400..35615)
                     /locus_tag="VF517_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053611.1"
                     /translation="MANQVTRREDAELRFREIAAQAGLPEPDEVVYHEDDEELEFLWH
                     EQKLAVVVELDENSGGAVDSAGPRSSQ"
     gene            complement(35735..38488)
                     /locus_tag="VF517_11530"
     CDS             complement(35735..38488)
                     /locus_tag="VF517_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053612.1"
                     /translation="MRRGGLLLVLALLLAVPGSAFAAPAKSVRGDAATGSQRSMLKAR
                     SVKVLAKSRHSGYVRLFATFRPYARKGPWQVITGIKEVELRKGSERRVTLRLNRIGRS
                     VMKGCTGGRIVVSAARIEGRAGAPIGKTIRTFKKLRRDAASCRGAAGGGGGAGGGTGG
                     GGSGGGSGGGGGSGGGGSGGGSGGGSGGGGSGGSGGSSVPAGEPVDFNPADPDRCDWL
                     DEADCLFPFPNDHFTVADSTTDTKRRLNLNILSMPKNKAGKPFDPEPLNRNDGFGPGL
                     PIETKVSGLDTKEAFQRSKIVPVDDMADALRPTQPVVVIDTRTRARHLVWAELDANPT
                     NPAERALVIRPGRHFVEGRRYIVALRNLKDAAGKTLTPSLGFRVYRDGHPTTNAAVEA
                     RRKSFEGMFTTLAEAGIARDDLYRVWDFTVASERNMTERILAMRDDAFAQLGDTKLDD
                     LTVQGTAPKYRITKVTDDPDGDVARRVEGTYTVPCYLHLPACPAGARFVFDPTKTNGP
                     PTRLPGNTMEARFTCNIPKVAKDGPPARPSLYGHGLFGSRSEVGSGNVRAMGNEHNMV
                     FCATDWIGMSCTDTPTSPDDLQSIVQSALAGTPDNPPDCDVPNALTIETDLSRFPELV
                     DRVQQGILNFLYLGRLMVHNSGFNADPAFRIGAGGAGVLDTRRLFYDGNSQGGIIGGA
                     LMAVMVDGDRGVLGVPGMAYSTLLQRSVDFGTGEPPQPDPTDPESLLPQYAYPLYESY
                     PNQIQRQLVLALMQTLWDRGEANGYANHITTDPLRNTPAHTVLMHVALGDHQVAQLTA
                     ESEARTIGASMRVPWADPGRDLDRGDPVYGVPKIPSYPFGGSAIVLWDTGPFRQIPGG
                     TAGTPPPPNENVPPEAGHDPHSAPRGSPIGRIQKSEFLRIDGKVIDVCGARPCYAGGW
                     AGP"
BASE COUNT         5292 a        13920 c        13747 g         5603 t
ORIGIN      
        1 gccgtcggcg tgcgtgcgcc gcgggcgcag cccgagctcg gccaccagcg cggccgcgcg
       61 gtcgtgtccg gcgccgatcc acgtggcacc gaggtcgaag gggaagccgt cgtgggtgtg
      121 agtctcgagc cgaccgccca cccggtcggg acgcgcctcg agcacgacca ccgtccaccc
      181 cgcgctcgcg acgcgacgcg cggtcgcgag ccccgccagc ccgccgccga ccacgacgac
      241 gtcggcggat cgcgtgcgtc ccgcggtcag cggggcttcg cctcgtcgta gcgcgtgagg
      301 ccgtcgtccg ggatcggctc ccacggcgcg gcgtaggcca cgaacgagcg ccagctcggc
      361 cgcaccccgg ggtcgccgtc gaacgccccc aggcggatgc tcatctgctc gcggccgtcc
      421 gggctgcggc tgaacaggtg cgcgccgcac gagcggcaga agcacttctc gaagccgccg
      481 tcgccggggt cccagccgcc gaccagctcc tcgccctgga ggagccggaa cgtgcgcgcg
      541 tcgatccgcg cctggggcga ggccgccgtg cccgtccggc gctggcagcg cgtgcagtgg
      601 cagtagcccg cgtcggtggg cggctcggtc agctcgaagc ggacgccgcc gcagaggcag
      661 ccgccggtca tcgagcggcc gcctcgcgat ccttcagctc gcgccgcagg atcttcccgg
      721 aggccgactt cgggatcgcg tcgatctcct ccacgagccg gatcttcttc tgcggcgaga
      781 ccttctccgc gcaccactgc atgagcgcgt cggagtcgag cgagtcgccc gcgggcacga
      841 cgtacgcctt cggcagctcg ccggcctcct cgtcgggcac gccgataacc gccacgtcct
      901 gcacgtcggg gtggttgatc agcagcgcct cgagctcggc cggcgcgacc tggaagccct
      961 tgtacttgat gagctccttg aggcgatcca cgatcgtgaa gaacccgtcc tcgtccacca
     1021 cgccgatgtc gcccgtgtgc agccagccat cctcgtccac ggtgtggctg gtcgcctcgt
     1081 cgttgttgag gtaacccttc atcacctgcg ggccccggat ccacagctcg ccgcgctcgc
     1141 cggcctgcgc gtccgcgccc gtctcgggat cgaccagccg gcactcggtg ttcggcagcg
     1201 cctggccgat cgagccgggc cggttctcgc cgctcggccg gatgcagtgg gtcaccgggc
     1261 tcgtctcggt cagcccgtag ccctggatca ccgtgcagtc gagccgtttc gccacggcct
     1321 gcgccagctc gccgccgagc ggcgccgcac ccgacatgat ccccgtgatc gacccgagct
     1381 cgtagttgtc cacggcggga tgcttggcga gcgccagcgc gatcggcggc accacgtagg
     1441 cgcgcgtgat gcggtgctcc tggatcagct cgaggaactg gtcgaggtcg aaccgcggca
     1501 tcgtcacgat cgtcgccccc gcccgcaggc cctggttcat gatcacgacc atcccgtaga
     1561 tgtggaagaa cggcagcacg ccgatcagcc ggtcgtcctc cgcgatcgga tacgcgatct
     1621 ggttctgcga catgttcgcc acgaggttgc ggtgcgagag catcacgccc ttcggcagcc
     1681 cggtcgtccc gctcgagtag ggcagcaccg cgaggtcgcc gtccgggtcg atctcgacct
     1741 ccggtgcctc gtccggattc cccatcagct cggtgaacgg cgtcgcgccg tcgccctcgc
     1801 cgaccacgaa cacctcctcg atgccggcct tggcggccgc ctcgctcgcc accgcgagca
     1861 tcgggccgac cgtcaccagg aagcgcgcac ccgagtcctc gagctggtga gccacctcgc
     1921 tcgccgtgta gagcgggttc atcgtcgtgc acttaccgcc caccgaagcc accccgtgca
     1981 gcgcgatcgc gtactccggc aggttcggca tgtagaccgc gagcacgtcg cccttgccga
     2041 acccgcgtgc ggtgagcccg gccgccagcg cgcggatgct ctgcgcgagc tggccgtagg
     2101 tgagcgtgcg cccggacggg ccgtcgatca gcgcgggctt gtcggcgcgc tcgcccgcgt
     2161 gctcgagcac gaacgcggtc aggtcgaggt cggggatctc gacgtcgggc tcgggtccgc
     2221 ggtggatggt catcggggcg tctcctctgt gtgcatggtc agacgaggat ctcagaggcc
     2281 gccaccttcg gcgcctcgct ggtgatcctc tgcgtcaggt tgtagaagcg cggcacgtcc
     2341 gggtacagca gcggcttgcc gttggtgatc acggccacgg ccgtcgcccg ctgcgggtcc
     2401 gcccacgaga ggatgttcgt gaagccgagg tggccgaacg cgtgctgggt gtcgcgcccg
     2461 tacaggctga ggagctgtgc gccgagcatc aggccgtagc tgaagcgcgt cgggaagccg
     2521 agcgacaggt ccacctcgag gtgcgactgc tcggcgagcg cctggcggat cgtcgtcggc
     2581 tccatcacgc gcacgccgtc gagctcgccg ccgcggcgca acagctcgta gaagcgcgac
     2641 agctcgttgg cggtcgtgac gatgttcgcc gacggcacga tcgcggtgcg gaactccggg
     2701 gtgttggccg acgccaccag ctcctcgaac ggcagtccga gtgcgcgcgt catgaacgtg
     2761 gaaagcggcg gcagcgcgcg cgggccggtg aggtagtcgg ccgcgaccgt gtcgaggtcc
     2821 cgctcgggca cgccgtagtt cgtccacctg aaccccagcg ggtcgaggat ctcctcggcg
     2881 agcacggtgc ggatgtccct gcccgtcacc tgccggacga cctcgccgag gatgaagccg
     2941 cccgacacgg cgtggtaggc gagccggttg ccgggcttcg aggcgggctt cgcgtcgatc
     3001 agccaccgca cgatcagctc ccagtcgtgg gcgttctgca cgtcgagcgc ctcgcgcggc
     3061 aggttcggca cgccggagcg gtgcgcgagc acgtggccga tcgtgacgtc gtccttgccg
     3121 tggcgctcgt agccgtcgat gtactcgcac acgcggtcgt ggatgtcgag cagcccgcgc
     3181 tcgtggagca tgtgcacgac gacggcggtg atggccttgg cgccggagta gatgaggaac
     3241 ggcgtggcgg gggtgacggg caccttctcc acgccggcgc cgtcgcgagg cccgttgccg
     3301 cgcgcgtgtc cgatcgcgcg gtcgagcacc acgacgccct cccgccggac gcacacctgg
     3361 atcgccgggt gcacgccgct gcgataccag tcggtcgtgg ccttccagat ccggtcgacc
     3421 gccgcggcgc tcatgccggc ggccgccggc tcgtcctcgg gaccgacggt ggtgaccgcg
     3481 gccaggtcgc gcggcacgtc gatgcggcgc agcgggtccg gcaccccggg gatgcgcggg
     3541 acgaccggta ctgacgggat gtcggggagc ccggccaccg ggcgtggcaa tctagtgctt
     3601 atggccccga tcagcgacgc cctgcgccgg ggcgaggccg cggcgggacc ggcgctcgac
     3661 cggctgatcg ccgcgggccg cgagcacttc ggccgcgacg gctacgccgg tacgtcgctt
     3721 gacgcggtcg tggcggccgc cggtgtcacg aagggctcgc tctaccacca cttcaagggg
     3781 aaggccgacc tgttcgaggc cgtcttcagg cagcgtgcgg ccgagctgtc cgagcaggtc
     3841 gccgccgcgt cgctgcccga gcgcgacccg tggcaggccg cctacgcggg cgtgcgcgcg
     3901 tacctcgacg gctcgcagga ggcggcggtc gagcggatca tgctgctcga cgggccgagc
     3961 gtgctcggct gggagcgggt gcgcgagatc gaggcggagt acggcctggc gctcatcaag
     4021 acgtcgatcg ggcgcctggt ggacgcgggc gagatccggc gccccgacgt ggacgtgctg
     4081 gcgcacctcc tgttcggggc gataacggag ggggtgctgc tggtcacgcg ctcgcccgac
     4141 ccgagcgcgg cgcggcggac ggtcgagcgc gagatcaggc ggttggtcga cgggctgggc
     4201 gagccggcct agctacggct ccacgagccc ccggcggatc gcgtagcggg tcagctcgac
     4261 ccggtcgcgc atgccgagct tgtcgaggat gtggccgcgg tggcgctcga cggtcttctc
     4321 gctgatggag agcgtcccgg cgatctcccg gctcgtcagc ccctccgcca cgagcttcag
     4381 cacctcggac tcccgcggcg agagcgggtc ggtgggcggg tcgtcgctcg agggcgagcg
     4441 ttcgaggtgc tcgcggatga gggccctgac ggcccccggg tacacgaacg gctcgccgcg
     4501 catggtggcc cggcacgcct cgaccaggtc gcggtccacg accgacttca gcacgtagcc
     4561 cgacgcgccg gcccgcaggg cctcgaagaa gtactgctcg ctgtcgtgca tcgagaggat
     4621 cagcgtgcgc agttccggcc gctgctgggt gagctcgcgc gcgacctgga tgccggtcag
     4681 ccgcggcatc gacacgtcca ggatcgcgag gtcgatctcc ccggcgagcg cggccttgac
     4741 cgcctccgcg ccgtcgcccg cctcggccac cacctccagg tccgcctcgc ggttgatgat
     4801 gagcttgagg ccgtggcgca cgagcgcgtg gtcgtcggcg agcaggatcc gggtcttgag
     4861 cgggaccgtc atggcggcgc ggggagggtc agctgcacgg ccgcgccgcc gtccggcccg
     4921 gccccgatct ccaggtgcgc cccgatcatc agcgcgcgct cgcgcatccc tgtgagtcct
     4981 gctccctcct cgtggcccgc gatcccggtc ccgtcgtcga gcacgcgcag cgcgaccgcg
     5041 ccgtcgtcgc gggcgagctg gagctcgatg cgcgacgggt cgccgtggcg gatcgcgttc
     5101 gtcatcgcct cctgcgcgac gcggtagacg gccagctcct cgtcggactc gagcggcggc
     5161 aggccggggt cgatgcggca ggcgcactcg aggccggtct gcgcggcgag gcgatcgcac
     5221 aggttcgcga gcgcgctgcc cagcccgagg tcgtcgagcg cctccggccg cagccgctgg
     5281 ccgatccggc gcacctcctc gagcgtggag cgggccgact cccgcgcggc gcccaccgga
     5341 gcggccacgc ccggcggcac ctcccgcgag acgttctcga gctccagcag cagcgcggtg
     5401 aggcgctggc cgatctcgtc gtggagctcg cgcgcgagtc gcagccgctc ggactcctgc
     5461 gcctcgagcg cgctggacgc gctcgtgcgg cgctcgcgct cgagccgggc cagcatctcg
     5521 ttgaacgcct cggtcagctc gaccacctcg gcgctgtcgc cgtagatcgg gatgcgccgc
     5581 ccggggcgca gcggatcggc ggtgctcatc agcgcgcgca gccgcccgat cggcgcgagc
     5641 tcgcgccgca ggaccagcac gttcaccacg tagatcacga tcaaccccgc caggccggcg
     5701 gcgaactcga gcggggccac cgtcggcgat acgagcgcgg ccggcgagaa cgcgagcatg
     5761 gcccaggcga tcatcagcac agtcgcgttc gggaggaaga tccgccagaa gagcgatggc
     5821 ctcacgtggc caccttggca tatgggggcc gctcccacgg gcggatgggg ggtggccctc
     5881 atgtcagccc gctgacggcg ctccatgatc gagggcgatg acttccatct ccgatacgca
     5941 ggtccgcgtc ctcgacgcgg atcccgacct cgccgccggg ctgcccgcgg acgagctgcc
     6001 ggccgcgacc cacgcggcac tggcgcgcac ctacctcctg gcgaccggga cctggacgcc
     6061 gccggcgtcg tgggccggga gcgcctcgac cctcggcctg ctggtgctgg acggcctggt
     6121 gatgcgctcg ctcgagctcg gcgaccgcag ctcgaccgag ctgctcggcg agggcgacct
     6181 gctgcgcccc tggcagcgcg acaccgagga ggcgctcgtc gcgtgcagcg tccagtggcg
     6241 ggtgctcgag cccacgcgga tcgcggtgct cgacggccgc ttcgccctcg cgatcgcgcg
     6301 ctggccgcgg ctcaccgccg cggtggtggg acgggcgctg cgccgctcgc gcgcgcacgg
     6361 tgtgttcgcg gcgatctcac acgtgaaccg cgtggacgat cgcctggtgc tcgccttctg
     6421 gtacttcgcg gagcgctggg gccgggtcgg gccggacggc gtggccgtgc ggcttccgct
     6481 cacccacgag cagctcggag ccatgatcgg agctcgacgc ccgtcggtga ccaccgccct
     6541 gtcctccctc gcggcgcagg ggatcctcgt gccgcgcagg cgcggtgaat gggtgctgac
     6601 tcccacggca cgcctgcgta tcgacgggct tcccgaggcg gcacgcggcg gggcgcagca
     6661 cgcgcctcgc ctcgccgcct gagcgccggc ggcatccggc ggcgcggctc gtgcgatccg
     6721 gtgggatcat ctatctcctg acccgccgaa tcgcagcaat cgtgatcctg gccctggcgg
     6781 ccgcggtacc ggcggccggc gccgccgccc ctcccgcgac cccgcagctc ggctgcggcc
     6841 cgggctcgcg tccggagccc ggcatgcagg gccgcgtgtc cgccgccgac gtcgcctccg
     6901 gcgcggccga caggggcttc acctgcaaca ccacgcaggt cggccacgag ggcgcgtcgg
     6961 gcgggttcaa ggtggagcgc ttcgtggacc gcgcgggcca cgactgcgcc tactacgaca
     7021 cgacgctgct gttcccgctg aacgcccaga atctcggcga ccagcccacc ggcgtggccg
     7081 tgctcgacat gtccgacccg gcgaacccga agcgaaccgc cacgctcgcc acgcccgcga
     7141 tgcagtcgcc gcacgaatcg ctcaacctca gcagggagcg cgggctgctt gccgcggtcc
     7201 tcggcaaccc gggcgcggcg cccggcgtcg tcgacgtcta cgacctcaac gacgactgcc
     7261 gccagccaca gctccagtcg agcgcgccgg tcgcgttcct cggtcacgag agcggcttcg
     7321 cgcccgacgg caagacgttc tacgcgacct cgctgttcga cggccacatc acagccgtcg
     7381 acctgaccaa ccccaagacg cccctcccga tcacggtcat cgagagcttc tcgcacggca
     7441 tgaacacgag cgacgacggc aaccgcggct acatcgccgc gcgcgacgtg gggctccaga
     7501 tcctcgacct cagcgagatc caggcgcgca agccgctgcc gcaggtgcgc gagatcagcc
     7561 gcctcaggtg ggacacgatg tcgatcccgc agaacgcgat cccgatcacg atcaagggga
     7621 aggcgtacgt ggtggaggtg gacgagttca gcgtcgagag cggcggcggc agcttcccgg
     7681 aggggaacgg cccgcaggtg ggcgccgcgc gcctgatcga catctccgac gaggcccacc
     7741 cgcgcgtggt gtccgacatg cgcctccagg tgcaccagcc ggagaaccgc gggcagctcg
     7801 agggcgaccc gggcgcgagc agcttcgtgc agggctacgc gggccactac tgcaacgtgc
     7861 cgcgccgcaa ggaccccgag atcgtcgcct gcacgttcat caactcgggc ctgcgcgtgt
     7921 tcgacatacg cgaccccgag aagccgaagg agatcgccta cttcgtcgcg ccgccgcggc
     7981 cgagcccggt gatgggcgac cgcagcaact acgcgatgtc gaagcccgag ttcgtgccgg
     8041 agcgcgacga gatctggtac tcggacggca acagcggctt ctacgtggtg cggctcgcca
     8101 acggggtgtg gcggtccggc ggcgcgagcg actgcctgcg ccccgcgcgg atcggcttca
     8161 ggatccaccg cacgggtcgc aaccgcgtgg tccgggtcga cgcgttcgtg aacggccgcc
     8221 gcaagctcct gcgccgcggt cgcgacatca cgcggatcga gctgagcaag ctcccgcgca
     8281 agggctcgat gaaggtccgg atcgtggcga cgcacaacac cggctcgaag gtcgtcagca
     8341 cgcgcacctg gagcggctgc gtgaagggcc gcccgaccgt gcgccagatc cgccggcgct
     8401 agtcagcgcc gctgagtcgc ggccgcaccg cgccgcgcgg tcagcgtcgc gccggaacgc
     8461 agtcgtgcgc ggtcgcgcgt gcctcggcgt cggcctccag cagcgtgccg accgagtccg
     8521 cgggctgcac cggcatccgc tcgagcgtct cctccacgac cctcgcgatg tcgccgaagc
     8581 gcagccgccc ctcgacgaac tccgccaccg ccacctcgtt cgcggcgttc agcacgcacg
     8641 tcgccgtccc gccggcctcc gccgcggcgc gtgcgatccg caggcacggc gcgatccggt
     8701 cgtccaccgg ctcgaacacg agcgggctcg cggcgaccag gtcgagtggc tcgaccgcga
     8761 cgtccacccg ctccgggaag tgcatggcgt aggagatcgg catgcgcatg tcgggcatcc
     8821 cgaggtgcgc gagcacggca ccgtcggtga gtgtcacgag cccgtggact atcgaggtgg
     8881 ggtggaccag cacctcgatc cgctcgtacg ggagcccgaa caggtggtgc gcctcgatca
     8941 cctcgagccc cttgttcatc agcgtggccg agtcgatcgt gttcttcacg cccatcgacc
     9001 aggtcgggtg cgcgagcgcg tcctccacgg acgcctgcgc gacctcctcc ggcgagcgac
     9061 ccctgaacgg gccgcccgag gcggtcacga cgaggcgctc gaccgccccc ggcggccccg
     9121 cctcgatgag ctggtcgagc gcggagtgct cggagtccac cggcacgatt cgcgcgccca
     9181 tgcggcgggc gaggtccatc acgagcgagc cgccgaccac cagcgactcc ttgttcgcga
     9241 gtgcgagctg cttgccggac tcgagcgtgc tgatcgtggc gccgagcccc gcggcgccga
     9301 cgatcgcgtt cagcacgagg tcggcgtcgg tgctcgccgc cagctcggcc gccgcctcgg
     9361 gcccgctcag cacctcggcc tcgggccagg cggaggcggc gtcgcgcgcg gccgcgggat
     9421 cgtcgagggc gatccgccgg gcgccatggc ggcggccctc gtccaccagc ggctcccacg
     9481 tggagcgggc cgagaggccg gccagctcga ggtccggcga gcgcgcgatc acgtcgagcg
     9541 cctgccggcc gatcgagccg gtcgctccca ggaggacgat gcgcttcaag acgctgaaaa
     9601 gctagcggga gcccccgccc ggcgctcgcg gcgcgatgtg gcttaagcgt ttcctacgcg
     9661 tcgatgtggg aagatcaaaa acagggaggt ggtggcgtga gcgactccgc cggggggccc
     9721 cttcgagggg aagacgtcct aatcgagagc agcggcggtt tgctcggttg gctcgaggag
     9781 ccgaccgccg agcgaggggt ccgcttcgcc cgcggcatta gcgagtggga cgtttggacg
     9841 tgggccgaga tggccgccgc ggcccacggc gccgccgcgc ggatgagcga gatgcacggc
     9901 gggcgctcag gcacggtcgc gatcgtgatc cccaacagcc ccgagttcgt cgcggccttc
     9961 tacggcgcgc tcgcggcggg cctcacaccg tgcccgatcg cgccccccaa ggcgatcgac
    10021 gagccccagc gctaccgccg gcacgtggcc ggtctgctcg aggcggcgca gccggcgctc
    10081 gtcgtgaccg tcgccgagtg ccgcgacgcg ctggcgccga tcgcgctcga ctgcccgctg
    10141 ctcgagctca gcgccgaccg gaccgcgccg ctgcgcccgc tggcgccgcc ccccgacatc
    10201 gccctgctcc agttcacctc cgggtcgtcg ggccgcccgc gcgcggtcca ggtcacgcgc
    10261 ccgaacctcg aggccaacat ccgcgcgata atcgccaagt ccgcgatgga cgggcaggtg
    10321 gaccacgtag cgacctggct gccgacctac cacgacatgg gcctgatcgg gtgcctgctc
    10381 gcgacggcgg cggccacggc cgggctgagc atgctgaggg tcgaggactt cgtcaggcgg
    10441 ccgatgcgct ggctcgagtg cctcggggcc gaggacggcg cggcgacgat cacagccacc
    10501 ccgccgttcg ggttcggcct cgcgctcaag cgcgtcaccg acgatgacct cgccggcctg
    10561 gacttctcgc gctggcgcgt cgcgatcgtc ggcgccgagc gcgtcgacgc cgccgtgctg
    10621 cggcggttcc tcgagcgctt cagcgagaac cggctctccc cgtcggtcct ctgccccgcg
    10681 tacggcctcg ccgagggcac gctgctggtg agcgccaagc agggcccgcg cgcggccacc
    10741 gtcatccgcc cggactggtc caccctccgg ttcggcgagc ccgtcgaggt cgacgcggtc
    10801 accaccctcg ccgatctcga gacgctcggc gacggcgtcg actggctcgt ctcgtgcggg
    10861 gactgcccgg acggcatcgg cgtggagatc gtgggcgagg acggcgcccc cctgcccgac
    10921 ggcacgctcg gcgagatcgc cgtcacgggc tcgtgcgtca ccgccggcta cctgaacgag
    10981 ccggaggtga cggcggagcg cttcgccggc ggtacgctgc ggaccggcga cgccgccctg
    11041 cgccacgacg gcgagctgta cgtcttcggg cgaatggcgg acgcgctcaa ggtcaacggc
    11101 cgctggctgt cgatcgagga gatcgaggcc aaggtgctcg cctccggcgc ggtcgcacgc
    11161 ggccgcagcg tgctgttctc cggacgcgac cccgagggcg acgcggtgat cgcgctgtcc
    11221 gaggccgagg ccggcgagtg ggtgagcgcc gtcgcggacg tgctcggcgc cgaggtgccg
    11281 gacacgatga gcatccacgt gctgagcggc ggctggggca cgatcatgcg tacgtcgagc
    11341 gggaagccgc gccggcgggc gatgtgggag gcgtacctcg cggacacgct cgacgccgag
    11401 gtcgtctact cgcgccgggc caacgcgccg gacgagcagc tcgccggagg gcgctccgca
    11461 tgaacgacac ccaggcgccg cccgggcacc gggacatgat ggccaggctt caggacctct
    11521 acgagggcag ccgcggcccc gggcgcacgc tgcgcgggga cgaccggctc gcggacgacc
    11581 tcggcatcga ctcgctccag ctcatggaga tgctggcgat cctcgaggag cagctcggga
    11641 tcgagatcgt cggcgacccg ggcttgttca acatcacgac ggtggacgac ttggtggagc
    11701 tgctcgcaca ccgcgtcgcc ggcgacaaac cacgatcgaa ggactgacca tgtcacctga
    11761 agacacgact gtcacatggc ccgcctcggc cgcgctcgtc ggcgacgagg accgtctcct
    11821 cgacggcacg accgaggtgc tcctcgctcc ggtgcgcgga cgcgatccca ggcgtgagct
    11881 cccgcgcgag ctgttccaca agcgccccgc gctgttcgtc ctcaagttcg gcttcgcgag
    11941 cctgctgatc gcggcctcgt gcgcggtgat cctcgcgggt ctggcctggc cgctccaggc
    12001 gcttgccgtc gtggtgctcg gcctcatgta cgcgcacctg gtcgagctcc agcacgagac
    12061 cctgcacgag cacgcgttca actcgcggcg gctgaacagg tggttcgggt tcgtctgcgg
    12121 gctcttcatg ttcagctcgt actcgcacta caagtacgac cacctccgcc accacgcgga
    12181 cctgggcacc gacaacaatc gcgagttctt caattaccgg tttcagaacc tgcaccgccc
    12241 ggatgggatg atccgcgcga tgtggcacat gggccgctac gccgacgtgt tccgggacat
    12301 cgtccgcagc atcgcgcggc gcccgctccc cgggatccgc gagcgccaca acaagcgcat
    12361 ccaggacgag taccggctgt tcgcggtggc gatcgtcgcc gcctgcgcgt tcacggccat
    12421 caccggcgac ttcgtgctcc tgctcctgtg gctcctgccg ctgctcgcga tcgccgagcc
    12481 gacgcacttc ctgatcgagc tcccggagca cttcgggctg aacacccaga gcaacccgga
    12541 cgtgctgtcc aacacgcgga ccgtcgcggc ggggccgctg ctgcgctggt tcacgaactc
    12601 caacaacctc cacacggccc accactacca ccagggggtc ccgatggtga acgtgccgga
    12661 gctccacggt ctgatcgacc atcaggtccg cgcggctgag gattcctact ggtcgttctt
    12721 cgcgaaggtg gcgaccggga agctcaggta cgaggacatg ggcgcgacct gcatgacgag
    12781 gtaggcacga tgaccaccac agcgacaccg acaacacccg cagcccacac caccgaggcg
    12841 ccgttccagg ggagcggctc gccgggccgg ccttcgcgca agtggatgct gaaggagctg
    12901 caccggctcg atcccgagaa ggacgccggc cggatcaccg agctgagcta cagcgcccgc
    12961 ttctcgcgga tgcagctctc gtaccacatg tactactcga tgttcttcct gcgggcgtcc
    13021 ggagcgccgg agagcgcgcg gccgatcgac cgcgacggga agggcttctt ctaccgcaac
    13081 ggccacaagc gcgccgacga cacgaccgcg gacatcttcg gctggatgca ccacggtccc
    13141 gagtcggagc tcggccgggc gtcgatcgcg cgcgtgaagc gaatgcacga caacctcgcc
    13201 gcgcgctggg ggatgccgaa ccacgtcctg gtgcacacgc tcgcgtgcga cctgctgtcc
    13261 ccgcactggc tgatggagat gatcgggggc ccgaagttcg acgaccacga gcgcctcgcg
    13321 cacctgaact tctggcgtgc ggtgggtgac gggctcggga tcaccgacgt cccggagacc
    13381 tgggacggca tggtcgccta cgtgaaggag tacgaggcct ccgagcactt ccagccctcg
    13441 ccgcacggca agcggctggc gcacggcttc gccgaccagt tctgcgagcg ctggttcccg
    13501 cgcgggttca ggtggctcgg gcgctgggtc ctcctgtcgc tggtcgagga ccacgtgctc
    13561 gacgtgcatg gcttcgagcg cccgccgcgg ccggtcgtga ggctgacgcg cgaggccacg
    13621 cgtctcacgg cgtacctcgc gtactacgtg ctgcgcgacc cctcgcccac gttcagcccg
    13681 ggcgagctgt tccgcagcgg gcacccggac atcgccgcct gcccggtgaa gcacgcgacg
    13741 ccctcctgat ggccctcctc gggtacccgc tggacgggtc cgccgagcgt gcggaccgct
    13801 gggagcagag cctcggcgtc atgccgttcg accccgcggt gaacatgctc ggttgggagg
    13861 agacgcggcg gcgcccggcg ctcgcgttgc gcctgcgcct ggcgctgctg gtgcgtcgct
    13921 tcgacgtcgc gctcagcgag gggctcaccg actggcgccg cctggcgccg aaggtgtcgg
    13981 cgggtcgcga gcgcatctac ttctggggct cggaggaggc gatagcggcg gcgcgctcct
    14041 ggttctccgg cggccgcctg ccggacgacg ggacgctggg cgggctcgag ctcggcacca
    14101 cgacgtacgt catggaggac cacgggccgg caacgatcgc cgtcgccgac cacgccgcga
    14161 gctgcaccac cgcggtgccc gcgatcggag tgggcctcgt cacctgcgac gagctgttcc
    14221 gcgagggcgt ggcgtcgatg cgcacggaga tccgcagccg ccacccggcg ccgggcgtgc
    14281 cgagcggcat ggccagccac ctcctggccg tccgcgagtt ccggctctcc tactggggcg
    14341 tcgcgccctg gtacatcggc tggggcgagc acgagatgct cggctacttc gagctgcccg
    14401 ccgatcccga gcgcacccgc cgcatgatcc tcgacgcgcc ggggccgttc atcgccgacg
    14461 cgtccgaccg ggccttcgcc gacgccctcg tcgcgctcaa cctcggcgag gaccgcccgg
    14521 cggcggccga cgccccgcct ggaacgaagg cgcaagcggg accgatccgc tgggaggtgc
    14581 acctgcccgg gccgctgctg atgccctggc acagggcggt ggtgcggccg acacggcgcc
    14641 gcgacggatc gacgctcgac gccgcgctgt ccacggtggc cgccacaccg cggaacgcgt
    14701 gcacgaccgt cgaggtgccg gtcgaggacc ccgccttcct cccggtccag cgccagctcg
    14761 tggcgtcggg cttccagctc gcggcgctcg cgcccccggt gcacatcgag ggcgaggggc
    14821 gccttgggtt cacgggcctc tggacgcgcg tcacgagcga gctgcccgtc gccgcgccgt
    14881 actacctgac ttcgaccctg ttgaacaacc gagagagcga ggtcgtgagc catgtccaca
    14941 ggctcagtga ccggtggacc ggacatcagc gcgcgtcccg ggccgcctga ggacggggcc
    15001 gggcgcgacc cggtcgcggt gatcggcgcc ggcgtctccg gcctcgtcgc gtgcggcgag
    15061 ctcgagcgcc gcgggatccc gtacgtctgc ttcgagaagg gcgaggggct cggcggcgtc
    15121 tggcgccggt accgcgacgt ggtcaccgtg acctgccgcg acgccatgga gctggaggcg
    15181 ctcccgatgc ctcccggcac gccggacttc ccgaccgggg accacatcat ccgctacatg
    15241 gaggactacg ccgacgcgtt cgagctgcgc gaccggatcg tgcacggcgt gtcgatagcg
    15301 agcctcgacc gcgagccgga cggccgctgg cgcgccgtcc actccgacgg caccagcacg
    15361 ctgcaccgcg cggtgatcct ggccacgggc cgcatcggcg agcccgcctg gccggagctc
    15421 gagggcagct tcgacggccg cttcctccac tcgggcgact accgcgacgg ctccgagttc
    15481 gagggcaagc gcgtggtcgt gatcggcttc ggcaactccg cggtcgacgt ggcgtgcgac
    15541 gtcgcgcgct acgcgagcac cactcacctg gcgatccgca ccggcacgtg ggtggtgccg
    15601 cgctacttcg ccgggcgtcc gctcgacaag gccagcggcc caatcgtgac gcgcgttccg
    15661 atggcgctgc gctggccgat ctaccgcgcg ctcctgtggg cgatccacgg caacatggag
    15721 gcctacgggc tgcccgcccc ggacgccaag ccgggtacgc ggccgctcac ggtcagcgac
    15781 gagctgatcc atcgcatcgg cgcgggccag gtgacgcccg cggccgcggc ggtcgcgctg
    15841 cgcggcgacc gggtcgagtt cgccgacggg agctcggtcg aggcggacgc tgttatctgc
    15901 gggaccgggt accgcatcga ggtgccgctc ctcgaggact acctggccgg gcgcgggctc
    15961 acgctcgacg gcctctggtt gaacgtcagc ccgcccgagg tggaggacct gtacgtcgtc
    16021 gggacgatgg tcgccttcgg cgcgatcccg ccgctggccg aggcgcaggc ggccctcgtg
    16081 gcggaactga tcggcggggc gggcgagccg ccgccggagc gggtcaagcg ccgcgagatc
    16141 gcgcaggaac gccggcgccg gcgccgctac tcgggggaca ccgcgcagtg gatggtcggg
    16201 gagactccgg cctacctccg ccggctgcgc aaggcgcggt ccgcggccag gcgccgcgcc
    16261 ggggccgagg gcagggcgac cgcgggctcg cgcgcatgag cacaccgccc ccagcgcggc
    16321 tggaggtcaa cggcctgatg ctctcgggct tcacggagct gggcttcggc gccatgaccg
    16381 gatggatcta cgcgctcgcg ctcgaggagc ccgacagggc gcgtgccatc gggatcaagt
    16441 cggtggaccg ggtccgccag tggcacctcg acctgatcgc gctgggcggt ctgtcggtgc
    16501 tcgtcggcac cgcgctgccg tcgctgccgc ggcgcgtctc ggttccgctc gccgtggggt
    16561 gctggacgaa cgccatgtcg ttcggcgtcc tgatggtcaa gcccgacctc accgagaaca
    16621 tggtgtaccg cgcggcgatc accggttcgt tcgcgatcac gagcgcgggc ttcgtcggcg
    16681 ccgctcgcga ggcgtggtcg cgccgcaggc gcccgccggc cccgcccgcc acgatctcgg
    16741 gaggtgggaa tggcgcgctg gatggcggtc gttgagtcgc gccggcgcct catgctcgtg
    16801 ctctggggga tcgtgttcgt ggtcgcgctc gtccccgccg ccaagcaggc cgaccatctg
    16861 accgccggcg gctacgaggt cccgggctcg ggatcgcacg ccgtcgagca ggccgcgcac
    16921 cgcttcccgc agctcaggcc gaacgcgatc ggcgtcctcg tcgatccgcc ggacggccgc
    16981 cgcatcgcgg ccgccgacgt gacgcgatca cgagccgaga tcgcgcccgc gatcgagaag
    17041 gtgccgagcc tctcgctcgg tgcgccgtcg accacgctcc tcgccccggg ggagaccgcg
    17101 gcggtgatcc cgctgcgcgg cccgcaggac gagaacagcg cgatcgacgc cgccgtcgag
    17161 ctgcgcgagc acatcgccga ggaggccggc gagaaccgcc tctacctggt cggccagccg
    17221 gcggtgtggg ccggccacga cgaggtgagc aaggagagcc tcgcgtccgc cgaggtcgtc
    17281 ggcttcccgc tgacgctgct gatcctcgtg gcggtcttcg gctcgctcgc cgccgccatg
    17341 ctcccgctcg cgctcggctt cgtcagcatc gtcgtctgcg gggcgctgat ctacggcctg
    17401 gccgagctga ccccgatgtc gctgttcgtg tcgaatgtcg cctcgctgat cggcatcgcg
    17461 gtcggcgtgg actactcgct tttcatcctc gcgcgctacc ggcgcgagct ggccgcgggg
    17521 cgcgaccaga gggaggcgct gcagatcacg atgcggacct ccgggcgcgt ggtgctcttc
    17581 tcgggcgtcg ccgtcgagac cgcgctcgcc ggcctgttcc tgatcgactc gctcgcgctg
    17641 cgctcgatcg cgctgggcgc gatggtggtc gtcgcggtgg cggtcgtggc cgcgctcacg
    17701 ctcacccccg cggtgatcct gacgatgggg gagcgcctgc accgtccctc ggcgcggatc
    17761 gcgagggtgc gctccgcgct gagcggcctc gtgcgccggc gctcgacgcc ggacgagttc
    17821 tgggcgcgct ggtcggatcg cgtgacccgg cgcccatggc tctcgctggc cgccgcgctc
    17881 gcggtcctgc tggtgctcgc cgcccccgcg ctgcggatga agacggtcac ccaggcgctg
    17941 ccggagctgc ccgcgagctc cgacgtgcgc accggagccg aggcggcgat cgagcggttc
    18001 ggccccgccg cgtcgggcga gacctacgtg ctggtgacgt acccgcgcag ggtcgacaac
    18061 gcgaccaacc gtcacatcgg ggccgtggtg gcggccgatc cagccgtcac ccacgtcgcc
    18121 ccgccggcgg tgagcggcga cggccgccac gcgctgttca gcatcgtgcc gcgcagcccc
    18181 gccgagaccg cggacactcg ccggctggcc gagcggctcg agggcaagct cgcgagcgtc
    18241 gccgctcgcg tgcccggcgt ctcgatcgcc gtgggcggga cggcggcgca cgaggcgcag
    18301 ttcgacgagc agatcacggg gtcgttgtgg aaggtcgtcc tgttcgtcct gctcgcgagc
    18361 gccgccgtga tgttcgtcct gctgcgctcg gtcctcctgc cgctgaaggc gatcgtgatg
    18421 aacgtgctga ccgtgggcgc ggcgttcggc gtcctagtgg cggtcttcca gtggggctgg
    18481 ttcgacgggt tcctcgggtt cgagtcgccg ggcgccgtct ccaccctcgc gctgccgttc
    18541 gtgctcgcga tcatgttcgg cctgtcgatg gactacgagg tgttcctgct cgccagggtg
    18601 caggaggagt accgccgctc gggggacacg cgctccgccg tccagcacgc gcttcgcacc
    18661 agcgccggcg cgatcagcag cgcggcggtg atcatgatcg tggtcttcct cgcgttcgtg
    18721 gccgtgagcc tcgcgactat caagatgatc gggctcggcc tcgcggtcgc ggtgctgctc
    18781 gacgcgacga tcgtccgtct tgtgctggtc cccgcggcgg tcgtgctgat gggcgacgcg
    18841 aactggtggg cgccctcgtg gctcaagcgc cgcgaccggg agacgccgcc gatggtcgcg
    18901 gcgggcggct agaccgagcg cggtccccgc cgcgccaccg ccgcgaggga cgacccctcc
    18961 cggtgcggca cgtccgggaa catccagccg aacatgaacg tgtcgatcgc cgtgccgggc
    19021 agcggcggca cggcgcgcca gcagcgctcg tgtccgctcg gagcgccgtc gcggcgccgc
    19081 ggccagtaga ggtggaacgc gggcagcacg tacatcgccg agccgtgcag gtccccgagc
    19141 ccggcgcggc ccaggtactc caccatctcg gcgggggtga acgcggcgcg ctcgctcgcc
    19201 agcgcgtcgt gcttgagctt gggcggcagg gggaagttga aggcggtctc catgaacccg
    19261 ccgtacgact tgccgtgacg caggcgggcc tcgtcgaaga tccagacggc gcacccggtc
    19321 cgctcgcgcg cggccgccat ctgctccagg cagcgctgga ggtggtcgaa cgacggcagg
    19381 tggtgcagcg actgcacgct gctcaccacc ccgaggtcgt cgggcaccag gtcggcgagg
    19441 tccgtgatgt cgccctggac catcttcacg cggtcgtcga ggccctcctc ggcgaacatc
    19501 gcctcggcgg tctcgatcat cagatcggag aggtcgacgc cggtgatcct caggtcgggc
    19561 cgcctgcgcg cgaggtgagc gaggttctgg cccgacccgc tgccgaggtc gagcacgtgg
    19621 gcgccctcgg gggcgaggtg gctcgtggcc agcgccgaga gctggtagat cgcggcctga
    19681 gtcttcttgc cgacctcgtg gtactcggcg accgagtccg cgtcgtccat caccatcggc
    19741 tcgggcacgc gggcctcgac cgaccgcgtc gagtactcgc gcagaaggga cagcgggacc
    19801 gtcgggacct tgctcagtcg catcgagagg cgcgcgagag tatcagtggc gtgccctgtc
    19861 ctggcgtgaa cgcatcactt gcgcctgggc caaggtgcac ttagggtcgt tgaggcaccc
    19921 cggtgcccgc ttatgcgtcc tctccttctg atcctcgccg ccctcttcgc cctcccggcg
    19981 gccgctcagg ccgacgtcat ccccttcggc agcgacctgt ccggcgcggc cgacgtcgac
    20041 cagtcgcacg gcgaggacac cgccttctgg aacgtcgcgc tggcgaacgg cggcgcggtg
    20101 accgcgccgg ccgagggcca catcacggcg atcaaggtga agggcggggt gctcacccgc
    20161 acgggcgaca acaagctcgc gcggcagatc cacttccagg tgctggaccc gaccgccgac
    20221 ggccccaagg tcagggtcag ctcggccgac ttctacctgc cgcgcacgaa cgaccagcag
    20281 gtcgtgacga cctacgagga gccggagctc gtcaacatct gcgtcaacaa gggcgacttc
    20341 atcgacctga acacgatcgg cggcgccgag ttcgccgcgg gcggcgacgc cggctcggcg
    20401 ctacaggtct tctcgcgcac gccaggctcc accgtcaact ggtacgagtc gggcggcggc
    20461 acgaacaacg gcgccgtctt cccgcggcag gtcgccctca acaacaccga gctgctgctc
    20521 caggcggtgc tctccaccgg gccggacgcg tcggacttct gccccggcgg ctaccgccag
    20581 cacatcttcc gtgggctcgc gttcccggcg ccgacccagc cgaccgtcaa gaccaagacc
    20641 cgcagggtgc ggctgaaggg cgagtgcaac ggcgagaact acggcggctg cttcggcgag
    20701 ctcacgctca gcgcggtgat cgacggcgcc tcccgcacgc tcggccaggt ggacttctcg
    20761 atcgcgcccg gcgtcaagaa cacgatcgag atcccgatct ccgaggcgag cgtgctcgcg
    20821 atccagaagg cgaagatcct caagggcacg ctcacggccg tggcgcacga caacccgcgc
    20881 ggcgacgcgc gcgtcaagtg ggacagcgtc ccgcgtcagg agaagacgac gaccgacgag
    20941 atctcgttga agccggacag gccggcctgc gtggtcccgg gcggcctgat cggcaagtcg
    21001 tcggcgtccg cgaaggcaaa gctcaaggcg gccggctgcg ggaacgtcgt gaagttcaag
    21061 aaggtcacga agtcgaagca gtggagcaag gtcgtctcgg tcaagccgaa ggcgggcacg
    21121 gtcctgccgg ccgggaccag ggtcacgctg accatcggcc gccgcaagta actccgccgc
    21181 gacgtacgct tcgaccatga ggtctctcgc gctggtctgc gcggtcgccg cgctgctgct
    21241 agccgcctcg ggcgcccagg cccagtcctc gttcgaccag aacggcgtgc tgttcgtgca
    21301 cgggttcgtg gggaccggag ggcagttcga gtcgcagaag ctccggttca cgagcaacgg
    21361 ctacccggag cgctgggtcg aggcgatcga ctacgactcg accttcgcga ccgagagccg
    21421 ggcgcaggtg cacgcgcgca tcgacgcgct catcgccgag ctgaagaagc ggaccgggcg
    21481 cccgaaggtg gacctcctcg ggcactcgct cggcacctcg gtcctccagg agtacctggg
    21541 ctcctcggcg gagcgggcgg cgaacgtggc ccattacgtg aacatcgacg gctcgcaggc
    21601 ggacgcgctt cccggcggcg tgccgaccct ggcgatctgg gccgggcgcg gggagccggg
    21661 ccggaggatc gtcggcgcga ccaacgtcac cctgccgaac cagacgcacg tccagtcggc
    21721 cacgtcggcc gaggcgttcg tcgagtacca caagttcttc accggcaggg cgcccgcgca
    21781 cgacatcgtc ccgcaggctg ggcagatcac gatcgccggc cgggtcctcc acttcccgct
    21841 caacagaggc gtggcggggg cgaccctcgc gatctgggag gtggacggcg ccaccggcca
    21901 gcgcacgggg agcgcacccc tcgccaccac gtccatcggc gagtccgggg actggggtcc
    21961 ggtcgaggtg acggcgggca ggcactacga gttcgaggtc acccgcccgg gcgctcccgt
    22021 gcaccaccac tactacgagc cgttcgtccg cagcgaccac ctggttcgcc tgctcgagtc
    22081 ggacgcgctg gccaacggcg gggagcgcgg gccgcgccac gccgccggcg tgatcctccg
    22141 ctacaaggag ctgtggggcg accagccggg ccagaacgac gcgctctcga tcaacgggac
    22201 caacgtctgc ccggaggtgg tgtgtccggt gagcaagcgg gtgaacgcgc tgttcttctc
    22261 cgaccggagg atggacggcc agagtgacct gagccagccg gaccccgtct acagcgcgct
    22321 gccgttcgtc accggggtgg acctcttcgc ccccgccgcg atcccgccga ccggacggac
    22381 cgccgtcgag atcaggtcgc gcggcggcgg gcccacccgg gcggtgaaca tgccgaactt
    22441 cgcgtcgctc accgacgtcg ccagcgtgca gctgaacgac tacgagcgca tcgacggcag
    22501 cggcccgtgc ctgcgcccgg cctcgatccc cttcaggctg caccgggtgg agggcacgcg
    22561 cgtggtccgg gtggaggcgt tcgtgaacgg caggcggacg ctagtgagcc gcggccgcga
    22621 catcaggcgc atcgagctgc gcaagctggc ccggaccgga acgctgaggg tccgcatcgt
    22681 ggcgacgcac aacacgggct cgaaggtcgt gagcacgcgg agctggagcg gctgcacgaa
    22741 gggcaagccg cgggtgcggg tggtccggcg ctccggagcc tgagaatggg gctagccggg
    22801 aggccgggtc cccaccccgt cgtcgacggg atacggactc cactcgtcgt ccggtaggcg
    22861 cagcgccgtc ggccaggtga gggggatgag cttcgacatg agcgcggcca tgtcctcgaa
    22921 gcacatgcgc acgacgtcgg cggcacccac cgggcggccg agcgcgagcg tccccacagc
    22981 ctccaggagc tgctcctggg tcatcgcacg cgcggacctg ccgcgcgcgg ggaccgcgcc
    23041 gagggccacc ggctcgccgt cgaggcgcaa cgtgccgatc cgcgagacgt aggcgaagac
    23101 gtcatcgacg ctcacgcccg cctcgtccat ggtgaaccgg gcgcggtcga ggcgaccgag
    23161 gcgatagccg agctcggcct tcgtcagctc gccgagttgt gccggcgtga gccagagcac
    23221 cgctgcccgc acggcggtcc ccgggctcgc gaagagcacg cctggcatcg acccgaacgc
    23281 ggtcggggac gcctgcgcgc cgacatccac tccgtgcagc tcgcctgtca ggacgagcgc
    23341 gtcccggtcc cgtgggtcct cgaacgacgc gaaccggtcc tggaggcgcg acggggcgcc
    23401 gttggccccg aatgccacga gcggatagcg gtcgcgggcg tgttcggcca cggcggcgcg
    23461 ccgctcggcc ggctcgatgt cctcgatcag gctgacctgg ccgccgcgca gcacgtagga
    23521 gccggagggc cgctcccacg ggtacccgag cgcatgctcg agggcggccg catcgtaggc
    23581 gcggctgccg aacgcacgga cgcgcttctc gagctccgcc agccaggtct cgtcgtccag
    23641 cgagatccac cgcagcgcct gcgggtcgtg gatgggcggc cagcgaccca tccgatgatc
    23701 ctagatgggt gggaggtggc gcgcccgcgc gtcgtcgacg acgacgagcc ccgcgtgagg
    23761 gttctggttg atcttggcgc agacgaaccc gttgccgttt cggtccgtgt cggcgtccgc
    23821 aggcggcacc gcctgcaccg gcgtcagtat gaaggagtcg ggacaggggt tcggcggcgg
    23881 ctgaggctga ctctgaccgg acgccgagcc aggtccgacc agcgccgcca cgaacagcgt
    23941 cgagctgacg gcgagcgcac gagttcctct gaccattctc attcctccat gatgtgtcga
    24001 taccgcctac gtcacgcccc taccccagcc cgttcggttc accctccccc gaccggagga
    24061 atcgagcggc ccggaaacgg caagcgcgct gcacgtaggc tgcccgcgtg agcacgctcg
    24121 ggatcgtcgg cggcatcggt cccccctcga ccgtcgcgta ctaccgcacg atcgtcgacc
    24181 tccatcgcgg tcgctgcggc cgtcccccgc gcgtggtcat cgacagcatc gacggcgacg
    24241 agctcgtgga gcatctggcc gcgggcgatc gccacgcggt cacgacgatg ctcgtcggcg
    24301 ctctcgacca gctcgctcgc gcgggtgcgg atctcgctct gctcgcatct gcatccgctc
    24361 acacggcgta tgacgaggtg cggtccgcct cgccgctccc gctggtcggg atcgtcgagg
    24421 cgactgcggc aggagcggtg gggtgcagtc gggtgggcat cttcgggacg cgattcacaa
    24481 tgcgcgccga cctgttcggc ccggcgctcg cggccgaggg gatcgcgacg gtggcgccct
    24541 caccgcagga gcaggaggcc atcgaccgtg tgtacttcga cgagctcgtg ggcggccggt
    24601 tcagacccga gtcgcgcgac gctctgcgcg caatcgcggt tcggctacgt gacgagagcg
    24661 gcgtcgacgg catcctcctg gccggtaccg agctcccgct cctcctgccc gaaacgcgct
    24721 acgacgacct cgtctacctc gacgtggcgc ggctgcacgc cgaggccgcc gtcgaggcga
    24781 tgctcgactg agccgtatct actcgaccac taccgtcggc tgcgagcgcg tctccgaccc
    24841 gcgcttactc ggccagttcc ttagcggcgg cgttcggccc gcggaaaccc gccgcgacct
    24901 agcgggcgac gcgcgtaccg ggggaaggtg gcggagtcaa cgccaggtcg cgtagtcgcg
    24961 gcggcggcgg gctggtcggc tcggccaggc ccagccactg caatgtcggc atcagatcga
    25021 gtccgagctg atccatcacc cgcatggtga cctttgcgat cacccagatc cccaaggcgc
    25081 tcaggagggt cacgacgaag aggaccatca gcatgcgccg gaattcggtg ccggtccgtg
    25141 cgttcctctt ggtagagagc ccctcggggg tggtgaagct cgcggcagcc gcgaggatgg
    25201 agctagccgg actcgaaccg gcgacctcct gggtgcgatc caggcgctct tccagctgag
    25261 ctatagcccc gcgccgggga tggtagcggc gcggaggccc cccggcggga tgccccgcgc
    25321 gccgcgcccg cgccccccgc gcaggctccg cgcgccactc ggcgaataga cgaagttggc
    25381 cgagtggcgt aacgggcaca ctttgtcgta cccctacggg ttccacctgc atgagctttc
    25441 gtagccgcct actgctgttc ttcacgatca tcgtggtcat cccgatggtc gcggtggcgc
    25501 tggtcctgtt cagcatcacc gaggacagcg agaccgggaa ggccgacgcc gagatcgccc
    25561 agggtctgcg ggcggcgttc ttcctctacg acgacgcccg ggacgacgcg cgctcgctgc
    25621 tcgacgacgt ggcgcgcgac cccgagctcg cgggagcgct gtcggagcgc gaccagaagg
    25681 cgatcgagcg ccggctgggg cagctgcgcg acaagcacgc gagcatcgtc gcgatcacga
    25741 tttacgacac ggcccggaag cggctcgcgc gcgacggcgc cgccgacgcg gtggcgttcg
    25801 ccgtcgccag cccctcggtg cgctcgggcg agcggctcgg cttcgtcggt gtctccgtca
    25861 cgcgcgcgtc tgacttcatg cagcaggtgc ggcggatgac cgcgctcgag gtgcgggtga
    25921 tcagccaggg ccgcgtcctc gcctccagcc tgaatgagcg cggtcgcgcg gtcgtggagt
    25981 ccggcgacgt cgacgtggcg gagcgcaagt accgcggccg cttcgccgtg gcccgggacc
    26041 ccgccggccc gaacgtgcag ctcggcctgt tcgtcgaggg cgcggagctc cgcgacgcga
    26101 tcggcgacca gcgcctcctg atcggcgcga tcctggtcgc cttcctgctg ctcgcgctgt
    26161 tctcgtcgat cgtggtcgtg cgcgcgctcc agactcagat cgcgcagttc ctcgaggcgg
    26221 cgcgccggct cgcccgcggc gacttcagcc ggcccgtgcc ggtcgagggt gccgacgagt
    26281 tcgccgagct ggccggcgag ttcaacgcca tgtccgcgca gctcgcgggc aagatcgacg
    26341 aggtcgagcg caagcgctcg gagctcgagg agacgatccg ccgcgtaggc gacgccttcg
    26401 cgaccggcct cgaccgcgag gcgatcgtga acctggtcgt gcgcaccgcc gtcgaggcgt
    26461 gcgaggccga gatcggccgg gcgctgccga tcgacgctcg tcgcatgcac cgcgccgagg
    26521 tgggcgagcc gtcacccgag ctcgtggctg cgctcgaggc cgccgagcgc agcgcgtttg
    26581 ccgtggacga cgacctcggc tcggactggc tcgcctcgct cgagggcaag gaggacatcc
    26641 accaccaccg ccccgtgcgc gcggaggtcg gcggcgtgca ctcgctggcc gcacagctcg
    26701 tcgcgcggct gggccgcggc ggcgcccagg agcaggtggg tgtggtctcg atcgcgcgcc
    26761 acggcggcgg cttcgacgac accgagtacg agctcttctc ctacctcacc ggccaggccg
    26821 cggtctcgat cgagaacgtc gacctccacg agatagtcca gctccaggca ctgacggacg
    26881 agctgaccgg cctcttcaac ctgcgccact tccacgacac gctcagcggc gagatcgagc
    26941 ggagccggcg cttcggtacc gatgtcggcc tcgcgatgct cgacatcgac gacttcaaga
    27001 agatcaacga caccttcggc cacccgcagg gcgacctggt gctgctcgag gtcgcgcgcg
    27061 tgctgcgctc gctctcgcgc gacatcgacg agccggcgcg ctacggcggg gaggagatgg
    27121 cggtgatcct gccccagacc gacctgcacg gcgccgagct gctcgccgaa cgcatgcgcg
    27181 ccgcgatcga ggcgctcgag atcaagcggc tcgacggcaa gggcacgctg aaggtcaccg
    27241 cgagcttcgg cgtcgcgtcg ctgccggtgt gcgccagcga caaggagagc ctgatcgccg
    27301 aggccgacgc cgcgctctat cgcgccaagc gggcgggcaa gaaccggacc gagcgcggcg
    27361 agccggtcac cgctgagggc taggcgccgc cggctccttt aacctgctgg ccgatgggag
    27421 cgctcgacga cgcgatccgc gagcacctgg agctcaagcg gcgtcacggt gcgacggagg
    27481 aggagatcgc gcgcaaggag gccgaggcgc tcggcccggc gcgccgcggg cccgcggccg
    27541 agcccgccat ggagcccgcg ccgggatctc cggaggatct gccgcccgag gcggtggatc
    27601 cggacgggct cgtcgagccc gaggcggtgg agctcgccga gcccgacgcc gccgagccgg
    27661 agccggtcga gccgcaggag ccggtagccc cgctcgccga aaccgagccc gtaggcgccg
    27721 acgaggagcc gctggccacg ccggtcgagc cgaccatcga cgtgccgtcc gagcccgagc
    27781 cgccggcggc cctcgaccca gccccggcgg acccgatcga ggccgacctc ccgcccgtcc
    27841 acgacgtgga ccgcgacccg ctggagcctc cgccgccggt cacgaaggcc gcccccggcg
    27901 ccaccgtcga gttcgacacg ctctccgcgc gccgcgacga cgacgatctc gccgacccca
    27961 ccgacgagga cgtcctcgag gagactccgg agttcctgcg cgagaccccc gagcacgacc
    28021 ggctctggtt cgagcagaag ccgccgcgcg acttcgacct cgataactga cgcccgcctg
    28081 gacaggtgtc cgagcctgag gcccaccggc tcgcgttgag ccgcgccgcg acgatgaagt
    28141 gagcacgacg gcatggaggg ttccgtcgtt cggtgcggcg tatcaagccg cttggtcagg
    28201 gggtcggcac ggcagccggc cccctttccg cctttctggg gtcagtcggc gtgcacgtac
    28261 ccgcgcgtgg tggcggcctc gaacacgcgc tcgatgccca gctcgtaggc ggccggacgc
    28321 agcgccaggt ccttctcctt ggcgcgctcg gacacctctc cgtaggcgcg gcgcatgatc
    28381 ttcgccagcc gttcgttgac ctcgtcctcc tcccagcgga agtgctggag gttctgcacc
    28441 cactcgaagt aggagacgat cacgccgccc gcgttcgcca tcagatccgg cacgaccatc
    28501 acgcccttgt cggcgagcat gtcgtcagcg gtcggcgtgg tcgggctgtt cgccccctcg
    28561 acgatcagct tgcagttgag cttgtcggcg ttgtgcttgt ggatcatccc gccgagcgcg
    28621 gccgggatga agacctcgca ctcggtctcg atgagctcct ccgccgagat ggcctccccg
    28681 ccgtcgaact cgcagacctt gcccccctcc ttcacgtgct cggcgagggc gtgggcgtcg
    28741 aggccctcgt cggagcggat cgcgccctcg acgtccgagg ccgcgacgag cttcgcgccc
    28801 aggtcctgga ggatccgcgc cgcccacgag ccgacgttgc cgtagccctg cacgacgaag
    28861 cgcgtgtcct ccggcttcag gtcgagctcc ttggccgcct cctcgtacat gtggaccacg
    28921 ccgcggccgg tggcggcctc gcggccgtag gagccctcga gggcgatcgg cttgccggtg
    28981 acgcaggcgg gcgtgtgccc gtagatcttc ccgtactcgt ccatcatcca ggccatcgcc
    29041 tgcgcgtcgg tgccgacgtc cggcgccgga atgtcgcgct gcgggccgag cagcttgccg
    29101 atcttgctga tgaacgaccg cgcgaccttc tcgagctcgt cctgctcgag ctcgcgcgcc
    29161 gggcagttca cgccgccctt ggcgccgccg tacgggatgc ccgcgatagc ggtcttccag
    29221 gtcatgagcg aggcgagcgc gcgcacctcg tcgaggtcca cctcggggtg gaagcgcacg
    29281 ccgcccttgt acgggccgcg cgcgccgttg tgctgcacgc gaaagcccga gtagacgtgg
    29341 acctttccgt cggcgcgccg gatcgggatc tggacctgca cctcgcggta ggaggtcttg
    29401 aggacggtcg cgatgtcgtc gccgatgccg agccgctcgg cggcgtcgtc gaagtagtgg
    29461 ctgacgatct cgtagttcga gatctcggcg cgcgtggtcg tctcggcctc gttggcggca
    29521 cgcggcgact tgtcgctgcc gtcgccgtca gagctctcgt ggagctgctc cttggtctcg
    29581 ggggcgtcgt cggactcgcg cgcctcctgc tcggtgctgg tggtggtctc ctcgctcatt
    29641 ccgttctcct gcccggggcg gcggatgttc tcacccccgg gcgtcggggt gactcaccaa
    29701 tgccattgcg ccagatatcc tgagcggcgt gagagggggg caacgggggg tcatcgcggc
    29761 gctggccgca gccgcgctcg tgcctgcggt cgtggccgct ccggcgcacg gcgccgcgat
    29821 cgcctaccag gcggatgccg cgcactcggg cttcgtgccg gacggtccca acccgccgct
    29881 ggggcgcaag tgggtgaggc gcgacctggg cgtgcaggtc tcctacccgg tgatcgccga
    29941 gggcaaggtc ttcgtgaccg cgtacgccgc cgacgcgagc aactcgagct acgggagctc
    30001 gagccccacc ggaccgacgt tcctgcacgc gctcgaccgc ggcaccggcg cgaccgtgtg
    30061 ggtgcgccag gtcgccaccg gcgggctgcc ggcctacggg gacgggcggg tctacgtcct
    30121 tggacgcgag gcgctcgacg cggtctcggc ctcgaacggc gagcgcctct ggcgtgcgga
    30181 gatgcgcgac accgggggcg cggcgccggt cgccgacggc ccgttcgtct acgcgtcgga
    30241 tgcgcgcggg gcgcacgcgg tctcctcgga caccggcctg acggtgcaga gcgagtcgct
    30301 gcccaacagc ggcttcaccg tggtcggcga ccgggtgtac gtgacgcagt cgggctgcaa
    30361 gacgatcgcg ctctactcgc gcgggctcgc gcagcaggtg tggtcgcgcg cgggtccgtg
    30421 ctcgtcgtcg gcgccggtgc cggcggcgta ccacacgctg aacacgacgc cgccgaacga
    30481 gcggctgtgg gcgcgcgact cgaacggtca gaccggcctc gtggtcgacg cgctcacgag
    30541 cctaggcacc gagcagttca actccgaggg cgccctcgcg ctcgccggcg agctcgcgtt
    30601 cctgcgcaac ccggctggga tccaggcgcg caatcaggcg agcggcgtga tcgcgtggac
    30661 ggcgtcgccc gccacgcagc tgcgtggctc ggcgctggcg gtgcgcgacg cggtctggac
    30721 caccaccggg agtggcgagc tggtggcgct gcgtcgcagc gacggcgggg agctgttccg
    30781 caccgtcttg ctggcgcacc agctgacgac gtcggaccac gactccagtg cgtcgccgcc
    30841 tcactccggc atggcggcgg atgcgaacgg cctggtcgtg ccgtacgtga accggctggt
    30901 ggcgctcggc cccggatcgg ataccccggg catcgacgag ctcgacaaga tcccggcagg
    30961 ccagacgcag ctgagcgcgg ccatgaagcc gcgcgagctc gagttcgggg cgaaggtgaa
    31021 ggtgaccggg cagctgaaga gctccgactc gcaggcgcaa ccgcaccagc tcgaactcca
    31081 ggccgacgcg tatccctacg acggcgtgtg gcaggcggtc gacagcgcgg cgacgcagcc
    31141 gacctatgac tactcgctcg accaccggcc cgaccgcaac acgcgctacc gcgtggtgga
    31201 catctccacg tcgcccgccg tggtgtcgaa gaccttccag gcgtgggtgt acccgaagct
    31261 gcggtggcgc ttcggccgca gccgggtggg ggcggtgcgg atagccgcga cgatcgccgc
    31321 gcccgatcac ctcgcgctga acgggaagcc gctgtacatc taccgcgggc gtacgcggcg
    31381 gggcacgtac acccggatcg gctcactcaa gatcaagcgg cggaagccga cgagctacag
    31441 cgcggtcggc acccgccggg tgccgagcca ccgcccccgc accgactact tcttcgtctg
    31501 cttccggatc ccggacacca acgtgctgac ccgggcgaag ggccgcccag atcagtgcgg
    31561 gcgcaggcgc ttgtagggcc gcgctccggc tacgctcagt ccgcccgggg gcgtggtgct
    31621 aacgggaaca cggggccttt gcaaggctca gttgggggtt cgattccccc cgcctccatt
    31681 gcctggctta cttcggtgct tgccgacggg cttctcgcgg atccgccttt gatccagacc
    31741 ggcctgcggt gcgttgtcgg cccccgcttc tgcggctgca cggcgcgggc tggccggggt
    31801 acggcgctga atcccgtcgc gataccattc tggtatgagc aacaggcaga tcgcggttcg
    31861 cctcccggac gaggtggtga agttcgtcga tgcgatcgtg agcagtggtc gcgagcggag
    31921 cagggcggct gttgtcaccc gggcgttgca gagagaacgg cggagggtga tggctgaacg
    31981 cgatgccgcg atcttgtcgg ggggcgagcc cgatccggag ttgatcgccc tcgccgagca
    32041 catcgcgcgc ctggatcgcg acctctagtg cggccgatcc acgtcgctca cttggacaag
    32101 gtccgcccgg tactcgtgct cacccgtgag gtcgtgcgcc cacacatgaa tacggtgacg
    32161 gttgctccca tcacgaccac cattcgcggc ctctcgaccg aagtcgaggt cggtcccgcc
    32221 aacggcctcg agcaccgctc cgtcgtggcc tgcgatcacg tgaccacgat cccgaacgct
    32281 gcgctttcgt cgcagatcgg agtcttgctc gatggccagg agcaggctct ctccgacgcg
    32341 attcgcaccg ccctcgatct cgactgagca tcgacgatcg gtccttagcg gcttgcgtcg
    32401 tgcgagagca cgcgaatcgc ggaggaactt ggagttcgaa agcatcccgc gacctccatt
    32461 gctcgggaga tttcgctcag gccgtgcgcg cggttgatcc gcccgcttcg gtctcgggct
    32521 gccatccggt ctcctcgcgc acgcgcttgg gaagagcgcg taccgccgcg acatcgctcg
    32581 ccgcgtcagt gccgggaacg gattgggctg tatggcgacg ccggtctagc ccgatcggcg
    32641 gcagcagcat ccattgcaag ggaggcctat gagaaaggtc acctcgtcgt ctttatgccg
    32701 atggtccggt tgctcgagat ggtgaagcag gagatcacta gcacggcatc ggaggacgcg
    32761 cgcgcgacgg accagttcgt cgctgccccc gacctgcttg tactcgacga cctcgacata
    32821 ggccgggagg gcgattggag cctgcttcac gttgcggccg ctctccggcg gcggacgttt
    32881 cgcaagacgc gagcaactgt tctgacgagc gattgtcatc cctccaaact cgggcccgtc
    32941 tgggagtgag gcgtgatcaa ggcgctcacc gagatctgtg gcccagcgtt gaacgtggat
    33001 cctcgctacg agcctccact cgccgattca gacgtattgg cggccgtgtt gaaggcacgt
    33061 cgccgagccc gggcgagggc ctagaggggg ctggcttcgg ctctacgtcg cgccgccaga
    33121 tacgcccggg tccttgggtg gctcgggaag ctccccatgg gccgtctcca agcgcctgat
    33181 gtcctccagg tcggttgcgc ggccgccctg ccgcttgagc gccacgaggt cggcgagcga
    33241 gcagatcgct gccgggacgc tttccactcg acgcgtgtcg gcacgcgaca gcagcccgct
    33301 caaatcgagc ggcggggctt gttcggcaag caggtcgatg aggccatgcg acgtacgcag
    33361 gtggaggggc cacccggcag agggcaaccg ccgctcctcc ggtgagccgt cgggccgcgt
    33421 cgcctcccac tcgacgcgga ggtcggccaa gcgcttggcc gtgtcctcgt cgcacgcgac
    33481 caccacgtcg aggtccttcg tcgcacgcac gacgccatga cggatgacag cgataccacc
    33541 gacgatgacg tagcggacgc ccgcgcggtt cagatcctcg agcaagcgcc cgaagccggt
    33601 cacacctcgg atcgctctgc cagttcggcg agctcgaggg caacttggct cagcgccatg
    33661 ccctcggcga gcagctcgtc cgggtcgcgg cctccgtcgc gcacgagctc gtattgccgc
    33721 catacctcgc ccgccgccca gccgtcgcct cgcctctcgg ccgcgcgcgc gtcagccgcg
    33781 agatcggcga ggcgtgtatc ccgttcggcc ctggtcacgc cacaagggta tgccgacgac
    33841 ctcgggcgcg cggcagagct aatagccggc cagctcccgt agccgtccct ccaggaaccg
    33901 ccgctccgca tcgtcggtgg ccagcgccag cgcccgctcg tacgccgctc gcgcctcgtc
    33961 gcgacggccg agccggcgca gcaggtcggc gcgcgtggcg tggaggtagc ggtagccgtc
    34021 cagcccctcg atcgcgtcga ccagggcgag ccccgctgcg gggccgtccg cctcggccac
    34081 ggcgaccgcg cggttcagct ccaccaccgg cgatcgcgtc agccgcaaga gcttcccgta
    34141 gagcgccgca atctcgtgcc agtcgcttgc tcgtcggcgt gcagcgaggc gatcgcggcc
    34201 tgcaccacgt acgggccgct cccgcgcagc gccagcgctc gctccagcag catccggccc
    34261 tcggccacgc gcccgccgtc ccagagcgag cggtcctggt cgccgagcag cacgagctct
    34321 ccatcccgga agcgtgccgc gcggcgcgcg tcgtgcagca gcatcagcgc gaggagcgcg
    34381 tgcacctccg gctcgtccgg catcagctcg gccagggcgc ggccgagccg gatcgcctcc
    34441 gacgcaaggt cgtggcgtcc gccgtagccc tcgttgaaga tcaggtacac gaccgccagc
    34501 accgccgcga gccggtcggg cagcaggtgg tcggggggta cccggaacgg gatgccggcg
    34561 tgcctgatct tgcgcttggc ccgcacgagg cgctgcgcca tcgtcgcgtc cggcaccagg
    34621 aacgcgcgcg cgatctcctc ggtcgtgagc ccgccgagcg ttcgcagcgt gagcgccacc
    34681 tgcgcctcgg tcgccagcgc ggggtggcag cacgtgaaca cgagctcgag acgctcgtcc
    34741 ggtattgcgg tgtcctcgtc catcggatcc tcggcggtcc gcgacggcac atcgagcagg
    34801 cgcatcttct ccgcgagggt ccgctcgcgg cggatgcggt cgatcgcgcg gttgcgtgcg
    34861 gtcgcgacca gccacgcgcg ggggttgcgg gggaccccct cgcgcggcca gcgcgcggcg
    34921 gcggtcgcga acgcctcctg ggcggcgtcc tcggcgagct cgaagtcccc gaggaggccg
    34981 atcagcgtgg cgagcacccg cccccactcg tcgcggaaga cgctctcgat cagcgctcca
    35041 ccaccgggcg cacctcgacg gcaccgctct gccgggcggc cgggatgcgt gctgcgatct
    35101 cgatcgcggc gtcgaggtcg tcggcctcga acaggtagta gccgccgagc tgctccttcg
    35161 tctccacgaa cggcccgtcc gtggtcagcg tctcgccgtc ccgcacgcgc accgtggtcg
    35221 ccgtcgagcc gggccgcagc tgctcgccgc tgaccttgcc gggaagctcg ccgatcgccc
    35281 cgtactcggc gagcgccgcg ttctgctcct cctcggagag ctggtcccag tcggtcgaat
    35341 tcgaccagat cagcatcatg tacttcatgg tgtttcctcc tctgtgaggg tcggtttccc
    35401 tactgggacg aacggggacc ggcggaatcg acagctccgc cggaattctc gtccagctcg
    35461 accaccaccg cgagcttctg ctcgtgccag aggaactcga gctcctcgtc gtcctcgtgg
    35521 tagaccacct cgtcgggctc gggcagcccc gcctgcgccg ctatctccct gaaccgcagc
    35581 tccgcatcct cgcgtcgtgt cacctgattt gccatgccgt gagtctgcgg cacggcgggt
    35641 tacgcgtggg gacgcgcgcg ttacaagttg gtgacgggcg agttacaact cggcgtcagc
    35701 cgcgggagcg ggcgtcaggc gcggagcggc ccgctcacgg cccggcccag ccgcccgcgt
    35761 agcacggccg cgcaccgcac acgtcgatga ccttcccgtc gatgcgcagg aactccgact
    35821 tctgaatgcg cccgatcggc gacccgcgcg gcgcgctgtg cgggtcgtgc cccgcctcgg
    35881 gcgggacgtt ctcgttcggc ggcggcggcg tgccggccgt gccgcccggt atctgccgga
    35941 acggccccgt gtcccacagg acgatggccg atccgccgaa cggatagctc gggatcttgg
    36001 gcaccccgta gaccggatcc ccgcggtcga ggtcgcgacc cgggtccgcc cagggcacgc
    36061 gcatggacgc gccgatcgtg cgcgcctcgc tctccgccgt gagctgcgcg acctgatggt
    36121 cgccgagcgc gacgtgcatc agcacggtgt gcgccggcgt gttgcgcagc gggtcggtgg
    36181 tgatgtggtt cgcgtagccg ttcgcctccc cgcggtccca cagcgtctgc atcagcgcga
    36241 gcacgagctg gcgctggatc tggttcgggt acgactcgta gagcgggtac gcgtactgcg
    36301 gcaggaggct ctcggggtcg gtcgggtcgg gctgaggagg ctcgccggtg ccgaagtcga
    36361 cgctgcgctg gagcagcgtg ctgtacgcca tgccgggcac gccgagcacg ccccggtcgc
    36421 cgtcgaccat caccgccatc agcgcgccgc cgatgatccc gccctggctg ttgccgtcgt
    36481 agaagaggcg ccgcgtgtcg agcacgcccg cgccgccggc tccgatccgg aacgccgggt
    36541 cggcgttgaa gccgctgttg tgaaccatca gccggccgag gtagaggaag ttcagtatcc
    36601 cctgctgcac tcgatccacg agctcgggga agcgcgacag gtcggtctcg atcgtgagcg
    36661 cgttcggcac gtcgcagtcc ggcgggttgt cgggcgtgcc ggcgagcgcg ctctggacga
    36721 tgctctgtag gtcgtcgggg ctcgtcggag tgtcggtgca cgacatcccg atccagtcgg
    36781 tcgcgcagaa gaccatgttg tgctcgttgc ccatggcgcg cacgttgccg gagcccacct
    36841 cgctgcggct gccgaacagc ccgtgcccgt agagcgacgg ccgcgccgga gggccgtcct
    36901 tcgcgacctt cgggatgttg caggtgaagc gcgcctccat cgtgttgccg ggcaggcggg
    36961 tcggcggccc gttggtcttc gtggggtcga agacgaaccg cgccccggcg ggacaagcgg
    37021 gaaggtgcag gtagcacggc acggtgtagg tgccctcgac gcggcgggcc acgtcgccat
    37081 cggggtcgtc ggtgaccttg gtgatccggt acttcggcgc cgtgccctgg accgtgaggt
    37141 cgtcgagctt ggtgtcgccg agctgcgcga acgcatcgtc gcgcatcgcg aggatccgct
    37201 cggtcatgtt gcgctcgctg gcgacggtga agtcccagac gcggtagagg tcgtcgcgcg
    37261 cgattccggc ctcggcgagc gtcgtgaaca tcccctcgaa gctcttgcgc cgcgcctcga
    37321 cggcggcgtt ggtggtcggg tgcccgtcgc ggtagacgcg gaacccgagc gacggcgtga
    37381 gcgtcttgcc ggccgcgtcc ttcaggttgc gcagcgccac tatgtagcgg cggccctcga
    37441 cgaagtggcg cccgggcctg atgacgagcg cgcgctcggc cgggttcgtg gggttggcgt
    37501 cgagctccgc ccacacgagg tgacgcgccc gcgtgcgcgt gtcgatgacg accaccggct
    37561 gcgtcgggcg cagggcgtcg gccatgtcgt cgaccggcac gatcttgctg cgttggaacg
    37621 cctccttggt atcgagcccg gaaaccttgg tctcgatcgg caggcccggg ccgaagccgt
    37681 cgttgcggtt gagcggctcc gggtcgaacg gcttgccggc cttgttcttc ggcatcgaca
    37741 ggatgttcag gttgaggcgc cgcttcgtgt cggtggtcga gtcggcgacg gtgaagtggt
    37801 cgttggggaa cgggaagagg cagtcggcct cgtcgagcca gtcgcagcga tcggggtctg
    37861 cggggttgaa gtcgaccggt tcgccggcgg ggacgctcga gccgccgctg ccgcctgagc
    37921 cgccaccgcc cgaaccgcca cccgagccgc cgccggaacc tccgccgccc gagccaccgc
    37981 caccgccgga gccgcccccg gacccaccac cgccggtgcc gccgcccgca cccccgccgc
    38041 cgcccgccgc cccgcggcag ctcgcggcat cgcggcgcag cttcttgaac gtccggatcg
    38101 tcttgccgat gggcgccccc gcgcgcccct cgatccgcgc cgcgctcacc acgatccgac
    38161 cgccggtgca gcccttcatc acgctgcgcc cgatgcggtt gagccgcagc gtcacgcgcc
    38221 gctcgctccc cttgcgcagc tcgacctcct tgatccccgt gatgacctgc cacgggccct
    38281 tgcgcgcgta cgggcggaac gtggcgaaca gccgcacgta gccgctgtgg cgcgacttcg
    38341 caagcacctt cacgctcctc gccttcagca tcgagcgctg gctgcccgtc gccgcgtcgc
    38401 cgcgcaccga cttcgccggc gcggcgaacg cgctgccggg caccgcaagc agcagcgcca
    38461 gcacgagcag cagtcccccc ctccgcacgg gccgaagcct agtccgagaa tcgccacgcg
    38521 gcgacggtcg tctgaggtac ggtccgcgac gatgcacgac ga
//
LOCUS       DASVPL010000214         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_24905, whole genome shotgun sequence.
ACCESSION   DASVPL010000214 DASVPL010000000
VERSION     DASVPL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24905"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1030
                     /locus_tag="VF517_11535"
     CDS             311..1030
                     /locus_tag="VF517_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8053613.1"
                     /translation="MAERKRTILLVEDERSIVEPLAAALEREGFHTELAGTVAEALQK
                     AGEVDPDLVLLDLMLPDGSGYDVCRELRSRSSVPIVMLTARGDEADRVVGLELGADDY
                     VVKPFSAREVAARIRAVLRRAQSVPAEAPTGTDDAIQIGDVRLDPARRTVTRGGGDVD
                     LARKEFELLQYLMENAGRVVTRDRLLEEVWDMNWFGSTKTLDVHVSAVRKKLGDDPAA
                     PRYLHTVRGVGFRFTGPDEIK"
     gene            1027..2478
                     /locus_tag="VF517_11540"
     CDS             1027..2478
                     /locus_tag="VF517_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8053614.1"
                     /translation="MRLRAPVRLRTRLIAAFAYVLILVIVALAVPLSLNLSKRVDAEV
                     RAEAAGQAQLVAASAGGRMDDADELDRLANRTTESLGGRVVIVNARGRLLADSAGEGL
                     EDEDYATRPEIATALRGETSQGERHSDDLDQDLLFTAVPVVSEGRTTGAVRITQSVDA
                     VNREVRNDTLALVGVGAIALLLGLGVAALLAGSLSRPLRALAATARRVGRGDLDARAE
                     VTGPAEQREVAAAFNDMTERLSQALASQREFVANASHQLRTPLTGLKLRLEAAGIRAD
                     DPELERDLIAAEREADRLARLLDELLTLAREADRPASGTPVALREAAEAAHERWAGPA
                     AQGDHALELGDGPDLHVLASAHDIAIVLDNLVENALNYSPPGSAVAIEWSATEDRARL
                     AVLDEGPGVAPDEDGRLFERFYRGEASRGGAPGTGLGLAIVETLARRWGGSARIANRP
                     EGGARAEVELPALPTPDPSLPDGLEAQGYARRR"
     gene            2475..2951
                     /locus_tag="VF517_11545"
     CDS             2475..2951
                     /locus_tag="VF517_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053615.1"
                     /translation="MRSVALTCALGVAGFLAAAGIGIAANEITGDSVGLSAEPLAAGE
                     DLAPAEASRSREDADRAERRAREKRRARAAKRRRERRRDAAERREDAPAAATPAAPAP
                     APVTPSGGDDHGGGGGGGDDNSGSGSSGSGSSGSGSSGSGSGSDDSGSGRGRGGDD"
BASE COUNT          408 a         1178 c         1161 g          357 t
ORIGIN      
        1 cggctcgagc ggctccgggt cgagcggctc cggctccggc tcgaactcgg gcagcggcag
       61 cggctcggcc tccggcagcg gctcgagcgg cagcggctcg acctcgggca gcggctcgag
      121 cggcagctcc gacgacgacg gcagcggctc gcaccgcggc cgcggcagcg acgacaactg
      181 agcgggagat cccgctcgga cacggggcgg ggcggctcca gtcaccgccc cgtcccacct
      241 gcttccccga ctgccccggc cgcctcccag ccggggcggt cgcccgcccg ggcccctagc
      301 atccgagccg atggctgagc gcaagcggac catcctgctc gtcgaggacg agcggtcgat
      361 cgtcgagccg ctcgccgcgg cgctcgagcg cgagggattc cacaccgagc tcgccggcac
      421 cgtcgctgag gcgctccaga aggccggcga ggtcgacccc gatctcgtcc tgctcgacct
      481 gatgctcccc gatggctccg gctacgacgt ctgccgcgag ctccgctcgc gctcatcggt
      541 cccgatcgtg atgctcaccg cgcgcggcga cgaggccgac cgcgtggtcg gcctcgagct
      601 cggcgccgac gactacgtcg tcaagccgtt cagcgcccgc gaggtggcgg cccggatccg
      661 agccgtcctc aggcgcgcgc aatcggtgcc cgcggaggct cccaccggca ccgacgacgc
      721 catccagatc ggcgacgtgc gactcgatcc ggcccgccgg acggtcacgc gcggaggcgg
      781 cgacgtcgac ctcgcccgca aggagttcga gctgctccag tacctgatgg agaacgccgg
      841 ccgcgtggtc acccgcgacc gcctcctcga ggaggtctgg gacatgaact ggttcggctc
      901 caccaagacc ctcgacgtcc acgtcagcgc cgtgcgcaag aagctcggcg acgaccccgc
      961 cgcgccgcgc tacctgcaca ccgtgcgcgg cgtcggcttc cgcttcacgg gcccggacga
     1021 gatcaaatga ggctgcgggc gccggtcagg ctgcgcaccc gcctgatcgc agccttcgcc
     1081 tacgtcctga tcctcgtgat cgtcgccctg gcggtccccc tgtcgctgaa cctctcgaag
     1141 cgcgtggacg cggaggtgcg cgcggaggcc gccgggcagg cgcagctcgt ggccgccagc
     1201 gcgggcggcc gcatggacga cgccgacgag ctcgatcgcc tcgccaaccg caccaccgag
     1261 agcctgggcg ggcgcgtggt gatcgtcaac gcgcgcggcc ggctgctggc cgactcggcg
     1321 ggggaggggc tggaggacga ggactacgcg acccgcccgg agatagcgac cgcgctgcgc
     1381 ggcgagacct cgcagggtga gcgccacagc gacgacctcg accaggacct gctgttcacc
     1441 gcggtgccgg tggtcagcga gggcaggaca accggcgcgg tgcggatcac gcagagcgtc
     1501 gacgcggtga atcgcgaggt gcgcaacgac acgctcgcgc tcgtcggcgt gggggcgatc
     1561 gcgctgctgc tcgggctcgg cgtggccgcc ctgctcgcgg gctcgctgtc gcgaccgctg
     1621 cgcgctctcg cggccaccgc ccgccgggtc ggccgcggcg acctcgacgc ccgggccgag
     1681 gtgaccggac cggccgagca gcgcgaggtg gcggcggcgt tcaacgacat gaccgagcgg
     1741 ctgtcccagg cgctcgcgtc tcagcgcgag ttcgttgcga acgcctcgca ccagctccgc
     1801 acgccgctga ccggcctgaa gctgcgtctc gaggcggcgg ggatccgggc cgacgacccg
     1861 gagctcgagc gcgacctgat cgccgccgag cgcgaggccg accgcctggc acgactgctg
     1921 gacgagctgc tcacgctcgc gcgcgaggcc gaccgcccgg cgagcggaac ccccgtcgcg
     1981 ttgcgcgagg ccgccgaggc cgcgcacgaa cgctgggccg gtcccgccgc gcagggcgac
     2041 cacgcgctgg agctcggcga cggtcccgac ctgcacgtgc tcgcctcggc acacgacatc
     2101 gcgatcgtgc tcgacaacct cgtcgagaac gccctcaact actcgccgcc gggctcggcc
     2161 gtcgccatcg agtggagcgc gaccgaggac cgtgcccggc tcgccgtgct cgacgagggg
     2221 ccgggcgtgg cgccggacga ggacgggcgc ctgttcgagc gcttctaccg aggcgaggcg
     2281 agccgcggcg gcgcacccgg caccggcctc ggcctggcga tcgtggagac gctggcccgc
     2341 cgctggggcg ggagtgcgcg gatcgcgaac cggcccgagg gcggcgcgcg ggcggaggtg
     2401 gagctgccgg ctttaccaac tcctgaccct tcgcttcccg acggcttgga agcccagggc
     2461 tacgctcgca ggcgatgaga tcagtggccc tcacatgcgc gctgggagtg gccggtttcc
     2521 tggccgcggc gggcatcggc atcgccgcca acgagatcac cggtgactcc gtcgggctct
     2581 cggccgagcc gctcgcggcc ggcgaggacc tggcgcccgc cgaggcttcc cgctcccgcg
     2641 aggacgccga ccgcgccgag cgccgcgccc gggagaagcg ccgcgcgcgt gccgcgaagc
     2701 gccgtcgcga gcgccgcagg gacgccgccg agcgccgcga ggacgcgccg gcggccgcca
     2761 cgcccgcggc ccccgctccc gccccggtca cgccgtcggg cggagatgac cacggcggcg
     2821 gtggcggcgg cggcgacgac aacagcggct cgggcagcag cggctcgggc agcagcggct
     2881 ccggcagcag cggctcgggc tcgggcagcg acgattccgg cagcggccgg ggtcgcggcg
     2941 gcgacgacta gtcaggcccc gcgccatcga ttggcgggtt ggacacgcgt aagcgtgtaa
     3001 aaggctccaa tcgcgctgag tggagccgct gcagcggccc gccgtggtgc ccgcgggcgc
     3061 tacttccggg ccgcggcgcc gcgggcgcta cttccgggcc gtgg
//
LOCUS       DASVPL010000215        12868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_104625, whole genome shotgun sequence.
ACCESSION   DASVPL010000215 DASVPL010000000
VERSION     DASVPL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104625"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF517_11550"
     CDS             <1..415
                     /locus_tag="VF517_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053616.1"
                     /translation="HIAPYAGQTLRAGRLRIRVLGPPPRAPGPPPEDPNPRALATVVS
                     AGSFDLFLSGDAESPALLGYPLPRVEAMKVSHHGSADPGLPELLRRLRPQVAAIEVGA
                     DNSYGHPTPSTLAALAHAVPRVYRTDRDGTVRLTP"
     gene            440..676
                     /locus_tag="VF517_11555"
     CDS             440..676
                     /locus_tag="VF517_11555"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX8053617.1"
                     /translation="MLEIRPNCECCDRDLPPDSPDARICTFECTFCAACVEHRLGGRC
                     PNCGGNFERRPIRPPAALAANPASTRRVTRDAPC"
     gene            670..1593
                     /locus_tag="VF517_11560"
     CDS             670..1593
                     /locus_tag="VF517_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8053618.1"
                     /translation="MLRQGRRRRIALLVLAAVAVLLAVNTVLVERDTDPADPDIGRIL
                     ELPGGDVQVREDGDPDDPPLILVHGFQASLRWWDRVTPKLARDHHVVRIDLLGHGGSE
                     KPRDGYSMPEQGDLVAQVMERLGIRRAAVVGHSMGGQVVTALAERHRNLVDRLMVIDT
                     APENRFSRNRRTQAIAAFPVLGHAISRVTPDSAIRKQVDLAFADGTDVPDVIYDDPKR
                     ATFNSFTGSRDGSREYRDEKPLDERLAGSGIRLHVIFGSEDELVDPKAAEEWDVPGVR
                     VAVLPGRGHSPMVEEPERMARLIAEFARVRE"
     gene            1743..2699
                     /gene="holA"
                     /locus_tag="VF517_11565"
     CDS             1743..2699
                     /gene="holA"
                     /locus_tag="VF517_11565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX8053619.1"
                     /translation="MAALKPAYLVCGEDDTKIDAWRARVRRRAETENGPGALETFDAR
                     SESPEAVAASLCALTFGGGTRYVMVDGVESWKPGVLEPLERELAAPAPDTVLVLVARG
                     KAPEKLRKAVEQAGGELREYEAPKPRALPRWVAERAAEEGLTLDADASRTLVSVVGSS
                     QQRLSREVEKLALLAHPKDRLSAEQVRRFASGETSGQGYDLADALVAGDLEQTLRLAE
                     RLVGQGDRPGRLVYPILSGLRRALRAAELIDAGVSESQLASELRMPPWLAKRVAAQAR
                     RADRDALERALCAFADLEVKMRCGECVDDESGLTLALARSAA"
     gene            complement(2773..3039)
                     /gene="rpsT"
                     /locus_tag="VF517_11570"
     CDS             complement(2773..3039)
                     /gene="rpsT"
                     /locus_tag="VF517_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX8053620.1"
                     /translation="MANIASQEKRIQRSARERLENRRYTSTIKTYFRRLEDAVASGDD
                     DRVATEHRELVSTIDRAVKRGALHRNTGARKKARAAKLRAGSSS"
     gene            3029..4732
                     /gene="murJ"
                     /locus_tag="VF517_11575"
     CDS             3029..4732
                     /gene="murJ"
                     /locus_tag="VF517_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX8053621.1"
                     /translation="MFATGGEPSEDERKPDAEPEPTRAGEDSEPEPTRAAADPEPEPG
                     SGGRRLAVSTLFFSFATALSRVMGLVREVVAASMFGVKGPMSAFTIAFQVPNLVRALV
                     ADAALQGAFVPVFTELLEKGKRREAFKVASGLLTLIAMVLGALTLIFLIAAPPLIELV
                     TPGFDDEPVLRDLTTGLSQLMFPIVLLLALAGVTVGMLNSFDRFGAAAVAPVFWNLAI
                     IACLVGLTPVFHGDDDIYAYAIGVLAGTVIQLVLPLPWLRGTGGRFTFSLDWRNPHVI
                     RVLKLMLPVTIALGLINFSLLINSFFGTLVSEEAPAAIDKAFRIYMLPQGIFSVAIAT
                     VVFPTLARFAARREFDDLRSTMANGVRLICLLLIPSAAFMAVLAEPITRVVYERGEFD
                     PAATDLVAEAMFWWAFSLPAQGVSLLLSRTYFSLQRPWMTTALAGGNMVVNAIVALAL
                     YEPLGVGGIVIGTVVGTVGMALAQAWYLRPDLGGVEGAQTLRAVVRMLLAAGILAAVA
                     YLTWRALDETIGRAIWSQAAAVAAAIVTGTAAYAAAVWLLRVEEAKQIVRLLAGRLGR
                     S"
     gene            4743..6572
                     /gene="lepA"
                     /locus_tag="VF517_11580"
     CDS             4743..6572
                     /gene="lepA"
                     /locus_tag="VF517_11580"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX8053622.1"
                     /translation="MAAPAANIRNFSIIAHIDHGKSTLADRILEVTGAVDARKHRPQL
                     LDKMELERERGITIKAQAVRVEYTARDGETYRLHLIDTPGHVDFTYEVSRSLAACDGA
                     LLLVDASQGVEAQTLANTYLAVDAGLELIPALNKLDLPGAEPERVAGEISELLGEPAD
                     DVIRISAKTGEGVTDVLEAIVERIQSPAGDLDAPPRALIFDSEFDQYRGVIAYVRVVD
                     GSLNKGDAIKAMQTGTQADIDDIGYFGPDLTPVASLSAGEVGYVITGVKDVSQLRVGD
                     TLTTRANPATEALPGYREVKPMVFCGLFPVETDQFSDLRDALEKLSLNDAALSWEPET
                     SQALGFGFRCGFLGLLHMDIVRERLEREYGLELLATTPNVEYEVTLTDGSVTTVHSPS
                     DMPDRARVDEIREPNIRATILCPKEHVGAVMDLCQERRGIHVDMSFLSEARVQLRYDL
                     PLAEIVLDFFDQLKSRTKGYASLDYELIGLRPADLVKLDILLAGDMVDALSMIVHKDK
                     AYAAGRGLTERLFKQIPRQQFEVAIQAAIGAKIIARESVKPMRKDVIAKCYGGDITRK
                     RKLLEKQKAGKKRMKQVGRIEVPQEAFLSVLTIGDSDNGAAKR"
     gene            6610..8619
                     /locus_tag="VF517_11585"
     CDS             6610..8619
                     /locus_tag="VF517_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8053623.1"
                     /translation="MLWQGLRRLLAPTVACVALLAPAAAQAADPPEGAEWKQAAIPST
                     DGVTLYADVLRPKGLPANAKTPVILSIGPYFNHSGQLGPLGLAQDAPYDPITGGPSDR
                     FYDFIKAAKVFEKGYTWVQVDLRGFGASNGCLDWAGPGEQADVKSAVEWAASQEWSTG
                     KVGMYGKSYDAVTGMLGVLTQPKGLAGVVAQEPVYDMYRYLYSNRVRYLNASATPNLY
                     NGIAASPGAASDSLAYQESALNDLQRPGCPAFNFADQQDPNHDSAYWKARNLIASADG
                     KQTPFFLTQGLLENNTKPDGAADYFNALDGPKRAWFGMWDHVRGTDKTSQGKFVMGRS
                     SFVEEAMRFYDRFVAGKPDSEAPVSKDPPVVLQTNDAKWRAEQNWPPKDAVALNAPLK
                     AGTYLDDGTEGGYNDGTGQNIWTFSPQFAHDVELAGTPRVTVDAETVAPSANLVVDLY
                     DVDKDGEAIIISRQAYLLPGSGKVSFNLYDQDWKMPAGHRIGVMVTGSNQGWWTHVPT
                     GQEVTVKSAQLSLPFLTCQRTATLPGETSVRLTNYARTEPFQLPADLIAANTLASFPT
                     PEAMRACTPVEQTGAGECVDKRKFLFRIKQPRGGRIVRATAYLDGKKILTVRGKRVTR
                     MTIAKFPKGTHTLRIVAQHASGKRTISTRQYKGCNKGRPTTVVRA"
     gene            complement(8616..9074)
                     /locus_tag="VF517_11590"
     CDS             complement(8616..9074)
                     /locus_tag="VF517_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8053624.1"
                     /translation="MKAIPNSLIRTSYRIGYHGLHAYWRVANPHKRGVKCVVTRGGEV
                     LLVRHTYGPTARWELPGGGVKRGEEPAAAARREAHEELGVDTPDWRPLGDLFERIDGK
                     RDTLWCFTTELPGSQEVITDDVEIAEARWFGRDALPADAHRYVRRILALG"
     gene            9096..9746
                     /gene="msrA"
                     /locus_tag="VF517_11595"
     CDS             9096..9746
                     /gene="msrA"
                     /locus_tag="VF517_11595"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8053625.1"
                     /translation="MLFFREKTKMIDPESALPGRSQQMPVPPVHEVLGNPMLPPFPEG
                     LERAVFGMGCFWGAERMFWQADGVWTTAVGYAGGYTPNPTYEEVCSGSTGHTEVVLAV
                     FDPKLTSYDELLRIFWEGHDPTQGMRQGNDVGTQYRSLIHWHGDGQRDAAERSRDMFA
                     ERLRTSGFDDITTEIAPAGDFYYAEAYHQQYLHKVPNGYCGLGGTGVSCPVGLAAS"
     gene            9840..10232
                     /locus_tag="VF517_11600"
     CDS             9840..10232
                     /locus_tag="VF517_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053626.1"
                     /translation="MESYPGTAGPATSEDLARVADELLEHTRALRRQHEELRDALAGI
                     SAVAAHEPLDRDEDEADGGHQNGATPETLHPMVLQMALAGETREFARDQLHALDVEDA
                     DELVDDVFDRVDSQRPAHRRRLFTRRAG"
     gene            10243..11502
                     /locus_tag="VF517_11605"
     CDS             10243..11502
                     /locus_tag="VF517_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053627.1"
                     /translation="MRFRRADLRVESFEYVEVGAGLALLRLAGVWRASAPEGVRLLTV
                     RGRAEEELAALPEPPDDGAGVWRAAFSAGAEVLDGRTAFRLEAADGRVVELPAPVERG
                     VGAAAADPEAAAEPEAAAEPEAEPYAEPEPEHAVHAEPEPYAEPSRNGSSPAEDHLDA
                     ADRGGFLTYGSTAPISQESGRETAHDARALEAAERALHAERARHERTEAGLREQLRVM
                     VAETADFMGRLEGYEMRRAELEKELSWERLLHKETRRLQDEAERERDEAIERLGPVEA
                     ELARVRREVEASARSQRRLVEARERIGELERQLERQEGLLETARTVADRGIAQLTALE
                     DRLVRMRDEAGRLPAAGSNGHAPAAPAIAGPQQELIDRAFEEADRGTERLAWLERRVG
                     ELRDGIAAQAVAEPANRPRGRGRRFRR"
     gene            11579..11652
                     /locus_tag="VF517_11610"
     tRNA            11579..11652
                     /locus_tag="VF517_11610"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11613..11615,aa:Pro,seq:cgg)
     gene            complement(11705..12025)
                     /locus_tag="VF517_11615"
     CDS             complement(11705..12025)
                     /locus_tag="VF517_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053628.1"
                     /translation="MADQTENGEERLDWTEAEMHPMTASGFMLVVRGVAKHPMKVRLR
                     PTPIGIVPDEDGYRGVVVLGKSEEFTTQVETPWTAQIDTKEVGDKIELIGATKKEYFP
                     PKDG"
     gene            complement(12078..12572)
                     /locus_tag="VF517_11620"
     CDS             complement(12078..12572)
                     /locus_tag="VF517_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053629.1"
                     /translation="MRAGPRTLVVPCLVALASTAAACGETRKTGSVGDKLEAKRIEVT
                     VEKVDRRTPVPRSDITGLSLPSSGHRLVGVLVNVCNDYGAVVGQFDFELETSTGEATP
                     KYTARNYRESFQSVRDDCERGWIVFEIPEESRPTKVEFAFDESGTNRNTQDNLTARFE
                     WKVE"
     gene            12624..>12868
                     /locus_tag="VF517_11625"
     CDS             12624..>12868
                     /locus_tag="VF517_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053630.1"
                     /translation="MFGLEEWFAELAHGQALGLVLLGALLLGLRHASDPDHLAAVSTL
                     IASEPEGGVRRAGWLGLAWGMGHATSLALFGLPIVLF"
BASE COUNT         1840 a         4728 c         4518 g         1782 t
ORIGIN      
        1 ccacatcgct ccgtacgcgg gtcagaccct tcgcgccggc cgcctgcgca tccgcgtcct
       61 cggccccccg ccgcgtgcgc ccggaccacc gccggaggac ccgaacccgc gcgcgctcgc
      121 gacggtcgtc agcgcgggct cgttcgacct cttcctctcc ggcgacgccg agagcccggc
      181 gctgctcggc tacccgctcc cgcgcgtcga ggcgatgaag gtctcccacc acgggagcgc
      241 cgaccccggc ctgcccgagc tgctgcgccg cctgcgcccg caggtcgctg cgatcgaggt
      301 cggcgcggac aactcctacg gccatccgac gccgtccaca ctcgccgcgc tcgcgcacgc
      361 ggtgccgcgc gtctatcgca ccgaccgcga cggcaccgtg cgcctcaccc cctgacggag
      421 ggtcgattag tctcgcgcaa tgctcgagat ccgccccaac tgcgagtgct gcgatcgcga
      481 cctcccgccg gattccccgg acgcccggat ctgcaccttc gagtgcacct tctgcgcggc
      541 ctgcgtggag caccgcctcg gcggcaggtg ccccaactgc ggcggcaact tcgagcggcg
      601 cccgatcagg cccccggcgg cgcttgccgc caacccggcg tcgacgcgtc gcgtgacccg
      661 cgacgccccg tgctgaggca gggcagacgc cgccggatcg cgctgctcgt cctggccgcg
      721 gtcgccgtcc tgctcgcagt caacacggtc ctggtcgagc gcgacaccga ccccgccgac
      781 cccgacatcg gccgcatcct cgagctgccg ggcggcgacg tccaggtccg cgaggacggc
      841 gatccggacg atccgccgct gatcctcgtg cacggcttcc aggcgtcact gcgctggtgg
      901 gaccgcgtca ccccgaagct cgcccgcgac caccacgtgg tccgcatcga cctgctcggt
      961 cacggcggct ccgagaagcc gcgcgacggc tactcgatgc cggagcaggg cgatctcgtc
     1021 gctcaggtca tggagcggct ggggatccgc cgcgccgcgg tcgtgggcca ctcgatgggc
     1081 ggccaggtcg tgaccgcgct cgccgagcgc caccgcaacc tcgtcgaccg cctcatggtc
     1141 atcgacacgg ccccggagaa ccgcttcagc cgcaaccgac gcacccaggc gatcgccgcg
     1201 ttccccgtgc tcggccacgc gatcagccgg gtgacgcccg actccgccat ccgcaagcag
     1261 gtggacctcg cgttcgccga cggcaccgac gtgcccgacg tcatctacga cgaccccaag
     1321 cgcgcgacct tcaactcgtt caccggctcg cgcgacggca gccgggagta ccgcgacgaa
     1381 aagccgctcg acgagcgcct cgccggctcc ggcatccgcc tgcacgtgat cttcggcagc
     1441 gaggacgagc tcgtcgaccc gaaggcggcc gaggagtggg acgtcccggg cgtccgcgtc
     1501 gcggtcctcc ccggccgcgg gcacagcccg atggtcgagg agccggagcg aatggcccgg
     1561 ctgatcgcgg agttcgcgcg ggtgcgcgag tgaggtccac cggcggaaaa cgtcgacatt
     1621 cctgacttcg cccccccata cggtggtcaa gtcaggaatg ccggagagaa gcggtgtcgg
     1681 cggaggcgca gcccgcgcgg gctctgagcc tggccgccgc ccccgggctt agactcggcc
     1741 gggtggccgc cctgaagccc gcctacctcg tctgcggcga ggacgacacg aagatcgacg
     1801 cctggcgcgc tcgggtccgc cggcgcgccg agaccgagaa cggcccaggc gcgctcgaga
     1861 ccttcgacgc ccgctccgag tcgccggagg ccgtggccgc gtcactctgc gccctcacct
     1921 tcggcggggg cacgcgctac gtgatggtcg acggcgtcga gtcctggaag cccggcgtgc
     1981 tcgagccgct cgagcgcgag ctggccgcgc ctgcgcccga caccgtgctc gtgctggtcg
     2041 cccgcggcaa ggccccggag aaactgcgca aggcggtcga gcaggcgggc ggcgagctgc
     2101 gcgagtacga ggcgcccaag ccgcgggcgc tgccgcgctg ggtcgccgag cgggcggccg
     2161 aggaggggct gacgctcgac gccgacgcct cgcgcaccct cgtcagcgtg gtcggcagca
     2221 gccagcagcg gctctcccgc gaggtcgaga agctcgcgct gctcgcgcac ccgaaggacc
     2281 ggctgagcgc cgagcaggtg cggcgcttcg cgtcgggcga gacgagcggt cagggctacg
     2341 acctcgccga cgcgctcgtg gccggcgacc tcgagcagac gttgcggctc gcggagcgtc
     2401 tcgtggggca gggcgaccgt cccggccgcc tggtctaccc gatcctcagc ggcctgcgac
     2461 gcgcgctgcg cgcggcggag ctgatcgatg cgggcgtgtc ggagtcgcag ctcgcgtccg
     2521 agctgcggat gccgccgtgg ctggcgaagc gggtcgcggc ccaggcccgg cgcgccgacc
     2581 gcgacgcgct cgagcgcgcc ctctgcgcgt tcgccgacct cgaggtgaag atgcgctgcg
     2641 gcgaatgcgt ggacgacgag agcgggctca cgctcgcgct cgcgcggtcg gccgcgtagc
     2701 cggggcgcct gcgcgggccg gcgtcctcct ggcgtgcggg cggcgtccgc tgaggcggcc
     2761 gcggaggttt ctctagctcg aggagccggc gcgcagcttc gccgcgcggg ccttcttgcg
     2821 ggcgccggtg ttgcggtgca gcgccccgcg cttgaccgcg cggtcgatcg tcgagacgag
     2881 ctcgcggtgc tcggtggcca cccggtcgtc gtcgccgctc gcgacggcgt cctcgaggcg
     2941 gcggaagtaa gtcttgatcg tcgacgtgta gcgacggttc tcgaggcgct cgcgcgccga
     3001 ccgctggatc cgcttttctt gtgaggcgat gtttgccacg ggcggagagc ctagcgagga
     3061 cgagcggaag ccggacgccg agccggagcc cacccgcgcg ggcgaggaca gcgagccgga
     3121 gcccacccgc gcggccgccg accccgagcc cgagcccggc agcggcggcc gccgcctcgc
     3181 cgtctccacg ctcttcttct cgttcgccac cgccctatcg cgcgtcatgg gcctcgtgcg
     3241 cgaggtcgtc gcggccagca tgttcggcgt gaaggggccg atgtcggcct tcacgatcgc
     3301 cttccaggtg ccgaacctcg tccgcgccct ggtggccgac gccgcgctcc agggcgcctt
     3361 cgtccccgtc ttcaccgagc tgctcgagaa gggcaagcgg agggaggcct tcaaggtcgc
     3421 ctcgggcctc ctgaccctga tcgcgatggt actcggcgcc ctcacgctga tcttcctgat
     3481 cgccgcgccg ccgctcatcg agctcgtcac ccccgggttc gacgacgagc ccgtgctcag
     3541 ggacctcacc accgggctga gccagctcat gttcccgatc gtcctgctgc tcgcgctcgc
     3601 gggcgtgacc gtcgggatgc tcaactcgtt cgaccgcttc ggcgccgccg cggtcgcgcc
     3661 cgtcttctgg aacctcgcga tcatcgcgtg cctcgtcggc ctcacgccgg tcttccacgg
     3721 cgacgacgac atctacgcgt acgcgatcgg cgtcctcgcg ggcacggtga tccagctcgt
     3781 cctgccgctc ccgtggctgc gcggcacggg cggtcgcttc acgttcagcc tcgactggcg
     3841 caacccgcac gtgatccgcg tcctcaagct gatgctgccg gtcacgatcg cgctcggcct
     3901 catcaacttc agcctgctga tcaactcgtt cttcggcacg ctcgtatcgg aggaggctcc
     3961 ggcggcgatc gacaaggcgt tccgcatcta catgctcccg caggggatct tcagcgtggc
     4021 gatcgcgacc gtcgtgttcc ccaccctcgc gcgcttcgca gcccgcaggg agttcgacga
     4081 cctgcgctcg accatggcca acggcgtgcg cctgatctgc ctgctgctga tcccgtcagc
     4141 tgcgttcatg gccgtcctcg cggagccgat cacgcgcgtg gtctacgagc gcggcgagtt
     4201 cgacccggcc gccaccgacc tcgtggccga ggcgatgttc tggtgggcgt tcagcctccc
     4261 ggcccagggc gtcagcctcc tcctcagccg cacgtacttc tccctccagc gaccgtggat
     4321 gaccactgcg ctggccgggg ggaacatggt cgtaaacgcg atcgtcgccc tggcgctgta
     4381 cgagccgctc ggggtggggg ggatcgtgat cggcacggtc gtcggcaccg tggggatggc
     4441 gctggcgcag gcctggtacc tgcggccgga cctcggcggg gtggaggggg cccagaccct
     4501 gcgcgcggtg gtccgcatgc tgctggcggc gggcatcctc gccgccgtcg cctacctgac
     4561 ctggcgggcg ctcgacgaga cgatcgggcg cgcgatctgg tcgcaggcgg ccgcggtcgc
     4621 cgccgcgatc gtcaccggca ccgcggccta cgcggccgcc gtctggctgc tgcgggtgga
     4681 ggaggcgaag cagatcgtcc ggctcctcgc tggtcggctc gggcgctcct aacctctcgc
     4741 acgtggccgc acccgcagcg aacatccgga acttctcgat catcgcccac atcgaccacg
     4801 ggaagtcgac cctggccgac cgcatcctcg aggtcaccgg ggccgtcgac gcgcgcaagc
     4861 accgtccgca gctgctcgac aagatggagc tcgagcgcga gcgcgggatc acgatcaagg
     4921 cgcaggccgt gcgcgtggag tacaccgcgc gcgacggcga gacctaccgc ctgcatctga
     4981 tcgacacgcc cggccacgtg gacttcacct acgaggtgag ccgcagcctc gccgcgtgcg
     5041 acggcgcgct tctgctggtg gacgcctcgc agggcgtcga ggcccagacc ctcgccaaca
     5101 cctacctcgc ggtggacgcc ggcctcgagc tgatcccggc gctcaacaag ctcgacctcc
     5161 ccggcgccga gcccgagcgg gtggccggcg agatctcgga gctgctcggc gagcccgccg
     5221 acgacgtgat ccgcatctcc gcgaagaccg gcgagggcgt cacggacgtg ctcgaggcga
     5281 tcgtggagcg catccagtcc cccgcgggcg acctcgacgc cccgccgcgc gcgctcatct
     5341 tcgactccga gttcgaccag taccgcggcg tgatcgccta cgtccgcgtg gtcgacggct
     5401 ccctgaacaa gggcgacgcc atcaaggcga tgcagaccgg aacccaggcg gacatcgacg
     5461 acatcggcta cttcggcccg gatctgacgc cggtcgccag cctcagcgcc ggcgaggtgg
     5521 ggtacgtgat cacgggcgtg aaggacgtct cgcagctgcg cgtcggcgac acgctcacca
     5581 cgcgcgcgaa cccggccacc gaggcgctgc ccggctaccg cgaggtcaag ccgatggtgt
     5641 tctgcggcct gttcccggtg gagaccgacc agttctccga cctgcgcgac gcgctcgaga
     5701 agctctcgct caacgacgcc gcgctgtcgt gggagcccga gaccagccag gcgctcggct
     5761 tcggcttccg ctgcggcttc ctcggcctgc tgcacatgga catcgtgcgc gagcggctcg
     5821 agcgcgagta tggcctcgaa ctgctggcca cgaccccgaa cgtcgagtac gaggtcacgc
     5881 tcaccgacgg cagcgtgacc accgtgcact cgccgagcga catgccggac cgcgcgcgcg
     5941 tcgacgagat ccgggagccg aacatccgag cgacgatcct ctgcccgaag gagcacgtcg
     6001 gcgcggtgat ggacctctgc caggagcgcc gcggcatcca cgtggacatg agcttcctgt
     6061 ccgaggcgcg ggtccagctc cgctacgacc tcccgctggc cgagatcgtg ctcgacttct
     6121 tcgaccagct caagtcgcgc accaagggct acgcgtcgct cgactacgag ctgatcggcc
     6181 tgcgccccgc cgacctcgtg aagctcgaca tcctgctcgc gggcgacatg gtcgacgcgc
     6241 tctcgatgat cgtccacaag gacaaggcct acgccgccgg ccgcgggctc accgagcgcc
     6301 tcttcaagca gatcccgcgc cagcagttcg aggtcgcgat ccaggcggcg atcggggcga
     6361 agatcatcgc gcgcgagtcc gtgaagccca tgcgcaagga cgtgatcgcc aagtgctacg
     6421 gcggcgacat cacccgcaag cgcaagctgc tcgagaagca gaaggccggc aagaagcgca
     6481 tgaagcaggt cggccggatc gaggtgccgc aggaggcgtt cctgtcggtg ctgacgatcg
     6541 gcgactcgga caacggcgcc gccaagcgct gagtcccggc gcgtgaacgc gcgccgctaa
     6601 catcgccgcg tgctctggca ggggctccgc cggcttctcg cgcccactgt ggcgtgcgtc
     6661 gcgctgctcg cgcccgcggc cgcccaggcc gccgacccgc ccgagggcgc cgagtggaag
     6721 caggccgcga tcccctcgac cgacggggtg accctctacg ccgacgtgct gcgcccgaag
     6781 ggcctgcccg ccaacgcgaa gacgccggtc atcctctcga tcggcccgta cttcaaccac
     6841 tccggccagc tcggcccgct cggcctcgcg caggacgcac cgtacgaccc gatcaccggc
     6901 ggcccgtccg accgcttcta cgacttcatc aaggccgcca aggtcttcga gaagggctac
     6961 acctgggtgc aggtggacct gcgcgggttc ggcgcgagca acggctgcct cgactgggcc
     7021 ggccccggcg agcaggccga cgtgaagtcg gccgtcgagt gggcggcgag ccaggagtgg
     7081 tcgaccggca aggtcggcat gtacggcaag tcctacgacg cggtcaccgg aatgctcggc
     7141 gtgctcaccc agcccaaggg cctcgcgggc gtagtagcgc aggagccggt ctacgacatg
     7201 taccgctacc tgtactcgaa ccgcgtccgc tacctcaacg ccagcgccac gcccaacctc
     7261 tacaacggca tcgcggcgag cccgggcgcg gcgtcggact cgctcgccta ccaggagtcg
     7321 gccctcaacg acctccagcg gcccggctgc ccggccttca acttcgccga ccagcaggac
     7381 ccgaaccacg actcggccta ctggaaggcg cgcaacctga tcgcctcggc cgacggcaag
     7441 cagacgccgt tcttcctcac ccagggcctg ctcgagaaca acacgaagcc ggacggcgcg
     7501 gccgactact tcaacgccct cgacggtccc aagcgcgcct ggttcggcat gtgggaccac
     7561 gtgcgcggca cggacaagac gtcgcagggc aagttcgtga tgggccgcag ctcgttcgtc
     7621 gaggaggcga tgcgcttcta cgaccgcttc gtcgcgggca agcccgacag cgaggcgccg
     7681 gtgtcgaagg acccgcccgt ggtgctccag accaacgacg ccaagtggcg cgcggagcag
     7741 aactggccgc cgaaggacgc ggtcgcgctg aacgcgccgc tgaaggccgg gacctacctc
     7801 gacgacggca ccgagggcgg ctacaacgac ggcaccggcc agaacatctg gaccttctcg
     7861 ccgcagttcg cgcacgatgt cgagctggcg ggcacgccgc gggtgacggt ggacgccgag
     7921 acggtggcgc cgagcgcgaa cctggtggtc gatctctacg acgtcgacaa ggacggcgag
     7981 gcgatcatca tctcgcgcca ggcctacctg ctgcccggct ccggcaaggt gagcttcaac
     8041 ctctacgacc aggactggaa gatgcccgcc gggcaccgca tcggcgtgat ggtcacgggc
     8101 tcgaaccagg gctggtggac gcacgtgccg accggccagg aggtcacggt caagagcgct
     8161 cagctctcgc tcccgttcct cacctgccag cgcaccgcga cgctgcccgg cgagacgtcg
     8221 gtgcgcctca cgaactatgc gcgcaccgag ccgttccagc tcccggccga cctgatcgcc
     8281 gcgaacaccc tcgcgagctt ccccacgccc gaggcgatgc gcgcctgcac gccggtcgag
     8341 cagaccggcg cgggcgagtg cgtggacaag cgcaagttcc tcttccgcat caagcagccg
     8401 cgcggaggcc ggatcgtgcg cgcgacggcc tacctcgacg gcaagaagat cctcacggtg
     8461 cgcgggaagc gggtcacgcg gatgaccatc gcgaagttcc cgaagggcac ccacacgctg
     8521 cggatcgtgg cgcagcacgc gagcggcaag cgcaccatca gcacccgcca gtacaagggg
     8581 tgcaacaagg gcaggccgac caccgtcgtc cgcgcctagc cgagcgcgag tatccgcctc
     8641 acgtagcggt gcgcgtcggc cggtagcgcg tcgcgcccga accagcgggc ctcggcgatc
     8701 tcgacgtcgt cggtgatcac ctcctgcgag ccgggcagct cggtggtgaa gcaccagagc
     8761 gtgtcgcgct tgccgtcgat gcgctcgaac aggtcgccga gcgggcgcca gtcgggggtg
     8821 tccacgccga gctcctcgtg cgcctcgcgg cgggcggccg cggcgggctc ctcaccgcgc
     8881 ttgacgccgc cgccgggcag ctcccagcgc gcggtcggcc cgtaggtgtg gcgcacgagc
     8941 agaacctcgc cgccgcgggt gaccacgcac ttcacgccgc gcttgtgcgg gttcgcgacg
     9001 cgccagtagg cgtgcagccc gtggtatccg atccggtagc tcgtgcgtat gaggctgttg
     9061 gggatcgcct tcacgcgcga cctatgatgg acccgatgct cttcttccgc gaaaagacca
     9121 agatgatcga ccccgagagc gcgctgccgg ggcgctcgca gcagatgccg gtgccgccgg
     9181 tgcacgaggt gctcggcaac ccgatgctcc cgccgttccc ggaggggctc gagcgggcgg
     9241 tgttcggcat gggctgcttc tggggcgctg agcgcatgtt ctggcaggcc gacggcgtct
     9301 ggaccacggc ggtcggatac gccggcggct acacgcccaa ccccacctac gaggaggtct
     9361 gctcggggag caccggccac acggaggtgg tgctcgcggt cttcgacccg aagctcacta
     9421 gctacgacga gctgctgcgg atcttctggg aggggcacga tccgacccag gggatgcgac
     9481 agggcaacga cgtcggcacg cagtaccggt cgctgatcca ctggcacgga gatggccagc
     9541 gcgacgccgc cgagcgctcg cgggacatgt tcgccgagcg gctgcgcacg tcggggttcg
     9601 acgacatcac caccgagatc gctccggcgg gcgacttcta ctacgccgag gcctaccacc
     9661 agcagtacct gcacaaggtc ccgaacggct actgcggcct cggcgggacc ggcgtgagct
     9721 gcccggtggg gctcgcggcg tcctgagcgt cgctgtagcg cgccggttcc ggcttacgcg
     9781 ggcggctctt cgggcatatt gacccgacag tcatcgagac ggtccgaggg ggacgtcgaa
     9841 tggagtcgta tccgggcacg gcagggccgg cgacgagcga ggatctcgct cgggtcgccg
     9901 acgagctgct cgagcacacg cgggcgctga ggcgccagca cgaggagctg cgcgacgcgc
     9961 tcgccggcat cagcgcggtg gcggcacacg agccgctcga ccgtgacgag gacgaggcgg
    10021 acggcgggca ccagaacggg gccacgccgg agacactgca cccgatggtg ctccagatgg
    10081 ccctggcggg cgagacgcgt gagttcgcgc gcgaccaact ccacgctctc gacgtcgagg
    10141 atgcggacga gctcgtggac gacgtgttcg accgcgtcga ctcccagcgc ccggcgcacc
    10201 ggcggcggct gttcacccgc cgggccggct gagcccgcga cgatgaggtt ccggcgcgcg
    10261 gacctgaggg tcgagagctt cgagtacgtc gaggtggggg cggggctggc gctcctgcgg
    10321 ctggccggcg tgtggcgggc gagcgcgccg gagggcgtgc ggctgctcac ggtccgcggc
    10381 cgcgcggagg aggagctcgc cgccctgccc gagccgcccg acgacggcgc cggcgtctgg
    10441 cgcgccgcgt tctcggccgg cgcggaggtg ctggacgggc gaaccgcgtt caggctggag
    10501 gcggccgacg ggcgggtggt cgagctgccg gcgccggtgg agcgcggcgt gggtgcggcc
    10561 gcggccgacc cggaggcggc ggccgagccg gaggccgcgg ccgagccgga ggcggagccc
    10621 tacgcggagc cggagcccga gcacgcggtc cacgcggagc cggagcccta cgccgagccc
    10681 tcgcgcaacg ggtcctcgcc cgccgaggac cacctggacg cggcggaccg tggaggtttc
    10741 ctgacttatg ggtccactgc gcccataagt caggaaagcg gacgcgaaac cgcgcacgac
    10801 gcgcgcgccc tcgaggccgc cgagcgcgcc ctccacgccg agcgggcgcg gcacgagcgc
    10861 accgaggcgg gcctgcgcga gcagctgcgc gtgatggtcg ccgagaccgc cgacttcatg
    10921 ggccggctcg agggctacga gatgcgccgc gcggagctcg agaaggagct gagctgggag
    10981 cggctgctgc acaaggagac gcgccggctc caggacgagg ccgagcgcga gcgcgacgag
    11041 gccatcgagc ggctcgggcc cgtcgaggcc gagctggcgc gcgtgcggcg tgaggtggag
    11101 gcctcggcgc ggtcgcagcg gcggctcgtc gaggcgcgcg agcgcatcgg cgagctggag
    11161 cgccagctcg agcgccagga gggcctgctc gagacagcgc ggaccgtcgc cgaccgcggc
    11221 atcgcgcagc tgaccgcgct cgaggatcgc ctcgtgcgga tgcgcgacga ggcgggacgg
    11281 ctgcccgcag ccgggtcgaa cggccacgcc cccgccgccc ccgccatcgc gggcccgcag
    11341 caggagctga tcgaccgtgc tttcgaggag gccgaccgcg ggaccgagcg gctcgcctgg
    11401 ctagagcgcc gcgtcggcga gctgcgcgac gggatcgccg cgcaggccgt cgcggagccg
    11461 gccaaccgcc ctcgcggccg cggccgtcgc ttccgccgat aagcccacgc atcggctcgc
    11521 ccgcgctcgg cgccccacgg cgaaggccca aaccgtgcgg ctagactgac gcccacttcg
    11581 gggcgtggcg cagcctggta gcgcgcaccg ttcgggtcgg tgaggtcccc ggttcaaatc
    11641 cgggcgcccc gattagatcc ctctaagcgc tgcgtacggc ccacgcagcg ctgccggcgg
    11701 ctcgctagcc gtccttcggc gggaagtact ccttcttcgt ggccccgatc agctcgatct
    11761 tgtcgccgac ctctttcgtg tcgatctggg ctgtccatgg cgtctcgacc tgagtcgtga
    11821 actcctcgct cttgccaagc acgacgaccc cgcgataccc gtcctcgtcc ggcacgatcc
    11881 cgatcggggt cgggcgcagc cgcaccttca tcgggtgctt ggccacgccg cgcaccacga
    11941 gcatgaaccc gctcgcggtc atagggtgca tctccgcctc ggtccagtcc agcctctcct
    12001 cgccgttctc ggtctggtct gccatcaatc cctccgtccg tcgctcggag gcatgacatc
    12061 acatccttcg agcgctctca ctccaccttc cactcgaacc gcgcggtcag gttgtcctgg
    12121 gtgttgcggt tcgttcccga ctcgtcgaag gcgaactcga ccttggtcgg gcggctctcc
    12181 tcggggatct cgaagacgat ccagccgcgc tcgcagtcgt cgcgcacgga ctggaacgac
    12241 tcgcggtagt tgcgcgcggt gtacttcggc gtggcctcgc ccgtgctcgt ctcgagctcg
    12301 aagtcgaact ggcccacgac ggcgccgtag tcgttgcaca cgttcaccag caccccgacg
    12361 agccggtggc cgctgctcgg cagcgacagg ccggttatgt cgctgcgcgg gaccggcgtc
    12421 cggcggtcca ccttctcgac ggtcacctcg atccgcttgg cctcgagctt gtcaccgacc
    12481 gagccggtct tcctggtctc tccgcacgcg gcagccgtgg acgccagtgc gacgaggcag
    12541 ggaacgacca gtgtccgcgg gccagcacgc acggcgggca tgttacgagc gggacccggc
    12601 cgcatcgcct acggtcagtg ggaatgttcg ggctcgagga gtggttcgcg gagctggccc
    12661 acggccaggc gcttggactc gtgctgctgg gcgcgctgct cctggggctg cgccacgcct
    12721 ccgatcccga ccacctggca gcggtctcga cgctgatcgc gtccgagccc gagggcggcg
    12781 tgcgccgcgc cgggtggctc ggcctcgcgt ggggcatggg gcacgcgacg agcctcgcgc
    12841 tattcggact gccgatcgtg ctgttcca
//
LOCUS       DASVPL010000216         8963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_104679, whole genome shotgun sequence.
ACCESSION   DASVPL010000216 DASVPL010000000
VERSION     DASVPL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104679"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VF517_11630"
     CDS             complement(<1..633)
                     /locus_tag="VF517_11630"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8053631.1"
                     /translation="MRQLEITGVRSVEWREAPEPALGGDGEAIVRPLAVALCDLDSAF
                     LTGAVPVGDGFPFGHECVAEVVEVGDGVESVRPGDVVVVPFQISCGACEACEAGHTGS
                     CRTVPRGSAYGMKPLGGDWGGALADRLRVPFADAMLVPLPDGLEPAAVASVADNVPDG
                     FRTVAAPLAATPGAEVLVVGGFARSVGLYATAAAVALGASRVVYADTDEGR"
     gene            665..955
                     /locus_tag="VF517_11635"
     CDS             665..955
                     /locus_tag="VF517_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053632.1"
                     /translation="MAIQRGIEGSEPVHRDVLGPPVEVQEERRSLRTTRMTGGTLACP
                     ACDAPVAPGARVLTPADPLACPYCDHAAPTRDFLSLDAPTRPARVAVHVVRR"
     gene            complement(977..2614)
                     /locus_tag="VF517_11640"
     CDS             complement(977..2614)
                     /locus_tag="VF517_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8053633.1"
                     /translation="MSLFDVLDDPRPGREHLSVWNGSGFTTLTWDDWRTRGAEVAGAL
                     RRLGVQPGDRVGCLLTNRPEVCAAVLGVWMAGGTVVSIPIIARGMSPEAYMQQIRTVR
                     AEVGADLVLMEAQYGALLDPDALGGRSVAYEDLSGAPLRELTPPADGEVAFVQYSSGS
                     TSDAKGCMLTGRAIRRQLVALGEALELDPEVDRGASWLPLSHDMGFFGALMQSLWNGI
                     HVAIGPPERFLRSPGSWARDWVDFDANLTATPNFALDLLARRRQGAAAGPLPMRKLVI
                     GGERVEPSTLARVTLALARDGFDATSLTPAYGLAECVLAVTMKPIGTDPRVLTVAREA
                     LADGRLELVDGEAAGPQTTTLVSSGRPVGDAELRIAGDGEVGEICVRSSNLTCGYVNR
                     PDLNAARLADGELRTGDAGFLHDGELYVTGRLDDMLSIHGRNIYARDVEVALSDAEEG
                     IRRGTCVLLDVADGGLSRVLLLTEPASDVAAVGELDLLAQRIADAALRETGIHLDECA
                     FVARGKLPKTPSGKVKRFRCRELIEGDGEGILARVRV"
     gene            complement(2640..3125)
                     /locus_tag="VF517_11645"
     CDS             complement(2640..3125)
                     /locus_tag="VF517_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053634.1"
                     /translation="MEKGAASECAARASLTPVSWRPREDLSSADWTEQGRKLGLIGRN
                     VGWWIGDWLRYGNAAYGERYTRAARVTGYDVQTLMNMVYVASRVDTSRRREKLSWSHH
                     AELAALDPDAQERWLARAEAERLSVRCLREEVRREARALAAGEAAGETFVCKSCGHSF
                     S"
     gene            3393..3791
                     /locus_tag="VF517_11650"
     CDS             3393..3791
                     /locus_tag="VF517_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8053635.1"
                     /translation="MTASHPFRAAVEARDVDGMVALLAPDVVLHSPVKFQPFEGREAV
                     RALFRALFEVFEDFRYTDELAGDGTHALIFRARVGDRELEGLDLLRAGPDGLLSEVTV
                     MVRPQSGLQAVGQAVAERLGLVGAGGAGAG"
     gene            4014..4706
                     /locus_tag="VF517_11655"
     CDS             4014..4706
                     /locus_tag="VF517_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053636.1"
                     /translation="MEGNAAPTKAPDTAQRKRRPSEAPEKRTAQDLARALTKTSWLPG
                     DELRLSEWIEHGRRLGVIGRGVGWWIGDWLRYGNMKFGERYVRASRITGYDVQTLMNM
                     VYVATAYDDTQRRGSLSWSHHAELAALPAGERDRWLDQAERERLSVRCLREELRRERR
                     LEAGQAPEPAAEPAAIAAGADAAAPDGPAESSGVEHGGVAAAAAAPDVRCPQCGCRIA
                     DEPAAPAPLRVA"
     gene            complement(4719..6287)
                     /locus_tag="VF517_11660"
     CDS             complement(4719..6287)
                     /locus_tag="VF517_11660"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX8053637.1"
                     /translation="MSDPMPRSPNKFSRHQENSAFPNGAGAPDAAAPKGGGTLTVTPP
                     TPAHLEPHTPLERLEMLCDPGSVHVIRSVVRSYSLGDKAREGDGVVGASGTIAGRPIF
                     CYAEDGRFAGGSLGKAHAETIVRVLELAGDARAPVVGFIESAGARMQEGSSALGAYGK
                     VFKANVRLSGCVPQISIVTGSSAGGGCYSPALTDFVVMTKGTSMFLTGPGVVREVTGE
                     VVTPGDLGGHKVHSRNGVCQFVADTDLDAAVLARVLLSYLPQSAWERPPELEPSPPAA
                     GDPGAYIPRESSQIYDVREVANRIVDGGSLLEVSPRWARNMVTAFARLGGMSVGIVAN
                     QPRYLGGVIDAAASEKGARFVRTCNAYGIPLVVLVDTPGFMPGTKQESAGVIRRGAKL
                     LYAFIEAEVPKVTVVVRQAYGGGFITMNSKELGADFSFAWPRATIGIMGARQAVGITH
                     RREIAAADDPREELRVLAERYVTEHQSAQAAARDGVVDELLAPSETRERLIAALGAMR
                     SKPGGQGRSGNIPL"
     gene            complement(6400..7617)
                     /locus_tag="VF517_11665"
     CDS             complement(6400..7617)
                     /locus_tag="VF517_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HEX8053638.1"
                     /translation="MTTEAAESKPVPLATTTVAMLPEAQLEPALKPAGGAGDAALPDA
                     RNVLVTGGTGFLGAFMVHGLLRDTAATIHCLVRADDADAARRRVIDNLGRFGLPTPDD
                     PSRIEPVVGDVTQPRLGIADADHERLQGELDGIYHGAASLNWKKPYDDIKRTNVAGIR
                     SVLELSAGGERSLPVHHISTVAVLPFWGLPEAGEDFSLEHDGWLLGGYTQSKWVAERT
                     AQAAAERGFPVRVYRPGGVTGSSRTGHFAQGAYLEAFLKGCIQLRSGPDSDMPFDMVP
                     VDYVADALLHISRTPNAERTFHLTNPTNDGIQEIFDAIETYGYPLERRPFEEWKQGLL
                     SSPDLAQNALKPFDWFLGNVGEQQITPPRYLSTRTQAAVEDSDLRCPPIAELIPTYLD
                     HWVEIGFLDPPPR"
     gene            7698..>8963
                     /locus_tag="VF517_11670"
     CDS             7698..>8963
                     /locus_tag="VF517_11670"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8053639.1"
                     /translation="MGGPIARRPWRFLVVALVLTLVAGALGVGVADVLQPYNGDPDPG
                     AESAAAADQIREATGVDPRGGIVALVEPGGGARSPSGRAAVARVRAELEAERTVGRVV
                     SPTTPGGTPLASRDGRATYLVAFLRPGQAAAHRDAAERIEERFEGRRDVRIGGVGAGY
                     VEGSEIAEEDLARAEQLVFPLLLAAAFLIFRGGVAAMLPLFVGFLSVLWTLALLRVLG
                     EATDVSVFALNLVTSLALGLSIDFSLLLVSRFREELERAGGDERAVALQRTLATAGRT
                     VLFSSLTIAAALASLLVFPQRLLYSLGVAGLLAALMTATLALVVVPACLALLGPRVNA
                     LAPRRLQRAAEREARPAEAGFWYRTAQLVARRPLPVALAAAALMLALAAPVLGMRTTS
                     ADTPAELPEGATTREVDDDLRTRFTPANAS"
BASE COUNT         1152 a         3372 c         3242 g         1197 t
ORIGIN      
        1 ccgtccctcg tcggtgtccg cgtagaccac gcgggaggcg ccgagcgcga cggccgcggc
       61 ggtcgcgtag agcccgacgc tgcgcgcgaa gccgccgacg acgagcacct cggcgcccgg
      121 cgtggccgcg agcggcgccg ccacggtgcg aaagccgtcc ggcacgttgt cggccacgct
      181 ggcgaccgcg gccggctcga gcccgtcggg cagcggcacc agcatcgcgt cggcgaacgg
      241 cacccgcagc cggtcggcga gcgccccgcc ccagtcgccg ccgagcggct tcatcccgta
      301 cgccgacccg cgcggaaccg tccggcagct ccccgtgtgc cccgcctcgc acgcctcgca
      361 cgcgccacac gagatctgga acgggaccac gaccacgtcc cccggccgca ccgattcgac
      421 cccgtcgccg acctccacga cctccgccac gcattcgtgc ccgaacggga acccgtcgcc
      481 gaccggcacc gctccggtca ggaacgcgga gtcgaggtcg cacagcgcga cggccagcgg
      541 ccgcacgatc gcctccccgt ccccaccgag cgccggttcg ggcgcctcgc gccactcgac
      601 cgagcgcacc cccgtgatct cgagctggcg caccccccaa tcatgccgga cctagactga
      661 gggcgtggcc atccagcgtg gcatcgaggg gtccgagccc gtgcaccggg acgtgctcgg
      721 cccgccggtc gaggtgcagg aggagcggcg cagcctccgc acgacgcgga tgaccggcgg
      781 cacgctcgcg tgtccggcct gcgacgcgcc ggtcgccccc ggcgcccgcg tgctcacccc
      841 ggccgatccg ctcgcctgcc cgtactgcga ccacgccgcc cccacgcgcg acttcctctc
      901 gctcgacgct cccacccgcc ccgcgcgcgt ggccgttcac gtcgtccggc gctgaccggc
      961 gcctctccgg ccgcttctac acgcgcacgc gcgcgaggat cccctcgccg tccccctcga
     1021 tcagctcgcg gcagcggaag cgcttgacct tgccgctcgg cgtcttcggc agcttgccgc
     1081 gcgcgacgaa cgcgcactcg tccaggtgga tccccgtctc gcgcagcgcg gcatcggcga
     1141 tccgctgcgc gagcagatcg agctcgccga cggcggccac gtccgaggcc ggctcggtca
     1201 gcagcagcac gcgcgagagc ccgccgtcgg cgacgtcgag cagcacgcag gtgccccgcc
     1261 ggatgccctc ctcggcgtcg ctcagcgcga cctcgacgtc gcgcgcgtag atgttgcggc
     1321 cgtggatgct cagcatgtcg tcgagccggc ccgtcacgta cagctcgccg tcgtggagga
     1381 agccggcgtc gccagtccgc agctcgccgt ccgcgagccg cgcggcgttc aggtccggcc
     1441 ggttcacgta accgcaggtg aggttggagg agcgcacgca gatctccccc acctcgccgt
     1501 cgccggcgat ccgcagctcg gcgtcgccca ccggccggcc cgacgagacg agcgtcgtgg
     1561 tctgcggccc ggccgcctcg ccgtcgacca gctcgagccg cccgtcggcc agtgcctcgc
     1621 gcgccacagt cagcacgcgc gggtccgtgc cgatcggctt catcgtcacc gcgagcacgc
     1681 attcggccag cccgtaggcc ggggtcagcg acgttgcgtc gaaaccgtcg cgggcgaggg
     1741 cgagggtcac gcgcgcgagg gtggagggct ccacgcgctc gccgccgatc acgagcttgc
     1801 gcatcggcag cggtccggcg gcggcgccct gtcgcctgcg cgccagcagg tcgagcgcga
     1861 agttcggcgt ggcggtgagg ttcgcatcga agtcgaccca gtcgcgcgcc cagcttccgg
     1921 gcgagcgcag gaaccgctcg ggcggcccga tcgcgacgtg gatcccgttc cagaggctct
     1981 gcatcagggc gccgaagaaa cccatgtcgt gcgagagcgg cagccaggac gcgccgcggt
     2041 cgacctccgg gtcgagctcc agcgcctcgc ccagcgccac cagctgccgg cggatcgccc
     2101 gtcctgtgag catgcagccc ttcgcgtcgc tggtgctgcc cgacgagtac tggacgaagg
     2161 cgacctcgcc gtcggccggc ggtgtcagct cgcgcagcgg cgcgccggac aggtcctcgt
     2221 aggcgaccga gcggccgccg agcgcgtcgg ggtcgagcag cgcgccgtac tgcgcctcca
     2281 tgagcacgag gtcggcgccc acctccgcgc ggaccgtgcg gatctgctgc atgtacgcct
     2341 ccggcgacat cccgcgcgcg atgatcggga tcgagacgac cgtccccccc gccatccaga
     2401 cacccagcac ggcggcgcag acctccggcc ggttagtgag caggcagccg acgcgatcgc
     2461 ccggctggac gccgagccgg cggagcgccc cggcgacctc ggccccgcgt gtgcgccagt
     2521 cgtcccacgt cagggtcgtg aagcccgagc cgttccagac cgataggtgc tcccgccccg
     2581 gccgcgggtc gtcgagcacg tcgaagaggc tcacgcgccg gcctcgcccg tcgcggctgc
     2641 tagctgaacg agtgaccgca ggacttgcac acgaaggtct cgccggcggc ctcgccggcc
     2701 gccagcgcgc gcgcctcgcg gcgcacctcc tcgcgcaggc agcgaaccga gaggcgctcg
     2761 gcctccgcgc gcgccagcca gcgctcctgt gcgtccgggt cgagcgctgc cagctcggcg
     2821 tggtggctcc acgagagctt ctcgcgacgc cgcgaggtgt cgacgcgcga tgccacgtag
     2881 accatgttca tgagcgtctg gacgtcgtag ccggtcaccc gggccgcgcg cgtgtagcgc
     2941 tcgccgtagg ccgcgttccc gtagcgcagc caatcgccga tccaccagcc gacgttgcgg
     3001 ccgatgagcc cgagcttgcg tccctgctcc gtccagtccg cggacgagag gtcctcgcgg
     3061 gggcgccagg agaccggcgt cagggacgcc cgggcggcac actcgctcgc agcgcccttc
     3121 tccaagccct gctgcgtagc ggaaagctga cccatgaccc ccctcgttgc gtcccctcgc
     3181 agggcggccg gtgggtcgcc cgcggtctcg ttccgagaat aatccgcaaa gtgcggttac
     3241 ggagcttcgg ggggtagatt acagacgttt ggggggccaa tggggcaacg gtcgcccaca
     3301 gcaccggtgc cggttgcgcc cacctcggtg ctggttgcgg gtcgccgcac cgcggaccac
     3361 actctgacga tcgccgagcc aaaggagagc cgatgacagc cagccacccg ttccgcgccg
     3421 cggtcgaggc gcgcgacgtc gacggaatgg tcgcgctgct cgcgcccgac gtggtcctcc
     3481 actcgccggt gaagttccag ccgttcgagg gccgcgaggc cgttcgggcc ctgttccgtg
     3541 cgctcttcga ggtgttcgag gacttccgct acaccgacga gctcgccggc gacggcacgc
     3601 acgccctgat cttccgggcg cgtgtgggcg accgggagct cgaggggctc gacctgctgc
     3661 gcgccggccc cgacgggctg ctctccgagg tcaccgtgat ggtgcgtccg cagtcgggct
     3721 tgcaggccgt cggccaggcc gtggccgagc ggctgggcct cgtcggggcg ggcggcgccg
     3781 gcgccggctg aggccgcgcg gggcgggcgc ggggcgggcc gaccgcgagc cgggggcgac
     3841 cttccgcggt cccccaaaga gtgtcgccgc gccgttttgg gggcgcctac caattagtgc
     3901 tgccttagcg gtaggatccc ccgaaccggg gataaccgga acgcggtgga tggctcgcca
     3961 tcgagtccgc cactcaggag ctgagcatca ccatccgggg ggggaaggtg aaaatggagg
     4021 gcaacgcggc gccgaccaag gcgcctgaca cggcacagcg caagcgccga cccagcgagg
     4081 caccggagaa gcgcaccgcg caggacctgg cccgagcgct gaccaagacg tcatggctcc
     4141 cgggcgacga gctgcggctc agcgaatgga tcgagcacgg gcgccgtctc ggggtcatcg
     4201 gccgcggcgt cggctggtgg atcggcgact ggctccgcta cggaaacatg aagttcggcg
     4261 agcgctacgt gcgcgcctcg cggatcacgg gctacgacgt ccagaccctg atgaacatgg
     4321 tctacgtcgc tacggcctac gacgacacgc agcgacgcgg cagcctctcg tggagccacc
     4381 acgccgagct ggcggccctg cccgccgggg agcgcgatcg ctggctcgac caggccgagc
     4441 gcgagcgcct gtcggtgcgc tgcctgcgcg aggaactccg gcgtgagcgc cggctcgagg
     4501 ccgggcaggc gccggagccc gccgcggagc cggcggcgat cgcggcaggt gccgacgcgg
     4561 ccgcgccgga cggtcccgcg gagagcagcg gggtggagca cggtggtgtg gcagcggcag
     4621 ccgctgcgcc cgacgtgcgc tgcccgcagt gcggctgtcg catcgcggac gagccggcgg
     4681 cgccggcgcc gctgcgcgtg gcctagcgcc tcctccggct agagcgggat gttcccggag
     4741 cggccctggc cgcccggctt cgagcgcatc gcaccgagcg cggcgatcag gcgctcgcgc
     4801 gtctccgacg gcgcgagcag ctcgtccacc acgccgtcgc gtgccgcggc ctgcgcgctc
     4861 tggtgctcgg tcacgtagcg ctcggccagc acccgcagct cctcacgcgg atcgtcggcc
     4921 gcggcgatct cgcggcggtg cgtgatgccg accgcctggc gcgcgcccat gatgccgatg
     4981 gtcgcccgcg gccacgcgaa cgagaagtcg gcgccgagct ccttcgagtt catcgtgatg
     5041 aagccgccgc cgtaggcctg gcggaccacg acggtcacct tcggcacctc cgcctcgatg
     5101 aacgcgtaga gcagcttcgc gccgcgccgg atgacaccgg ccgactcctg cttggtgccg
     5161 ggcatgaagc ccggcgtgtc gaccagcacc accagcggga tcccgtaggc gttgcaggtc
     5221 ctgacgaagc gcgcgccctt ctcggaggcg gcggcgtcga tcacgccgcc gaggtagcgc
     5281 ggctggttcg cgacgatgcc gaccgacata ccgccgaggc gcgcgaacgc ggtgaccatg
     5341 ttccgcgccc agcgcggcga gacctcgagc aggctcccgc cgtcgacgat ccggttcgcc
     5401 acctcgcgca cgtcgtagat ctgcgagctc tcgcgcggga tgtacgcgcc cgggtcgccg
     5461 gcggccggcg gcgagggctc gagctccggc gggcgctccc aggcgctctg cgggaggtag
     5521 gagagcagga cccgtgcgag caccgcggcg tcgaggtcgg tgtcggccac gaactggcag
     5581 acgccgttgc gcgagtgcac cttgtgaccg ccgaggtcgc cgggcgtgac cacctcgccg
     5641 gtgacctcgc gcaccacgcc gggaccggtc aggaacatgc tcgtcccctt ggtcatcacg
     5701 acgaagtcgg tgagcgcggg cgagtagcag ccgccgccgg ccgagcttcc ggtgacgatc
     5761 gagatctgcg gcacgcagcc cgacaggcgg acgttggcct tgaagacctt cccgtacgcg
     5821 ccgagcgccg acgatccctc ctgcatccga gcgcccgcgg actcgatgaa ccccaccacc
     5881 ggcgcccgcg cgtcgccggc cagctcgagc acgcgcacga tcgtctccgc gtgcgccttg
     5941 ccgagcgagc cgcccgcgaa gcgcccgtcc tcggcgtaac agaagatcgg ccgcccggcg
     6001 atcgtgcccg aggcgcccac gacgccgtcg ccctcgcgcg ccttgtcgcc cagcgagtac
     6061 gaccgcacca ccgagcggat cacgtggacc gaccccgggt cgcagagcat ctcgaggcgc
     6121 tcgagcggcg tgtgcggctc cagatgcgcg ggtgtgggcg gggtgaccgt cagcgttccg
     6181 ccgcccttcg gcgcggccgc gtcaggggcg ccggcgccgt tcgggaaggc cgagttctcc
     6241 tgatgtcggg agaacttgtt gggagatctg ggcatggggt cgctcatttc ttcccccatc
     6301 ctgcggaagt tcgcggagat ccgcaaagct ccccttagcg cgaagcgccg atcgtcgcgt
     6361 cccaggcccc gctttcggcg ccccgccggg cgcgcgcgcc tacctcgggg gcgggtcgag
     6421 gaacccgatc tcgacccagt ggtcgaggta cgtcgggatc agctcggcga tcggcgggca
     6481 acgcaaatcg ctgtcctcga ccgctgcctg cgtgcgcgtc gacaggtagc gcggcggcgt
     6541 gatctgctgc tcgccgacgt tcccgaggaa ccagtcgaac ggcttcagcg cgttctgggc
     6601 caagtccggg ctcgacagaa ggccctgctt ccactcctcg aacggccggc gctcgagcgg
     6661 gtagccgtac gtctcgatcg cgtcgaagat ctcctggatg ccgtcgttcg tggggttggt
     6721 gaggtggaac gtgcgctccg cgttcggcgt gcgcgatatg tgcagcagcg cgtcggcgac
     6781 gtagtcgacc gggaccatgt cgaagggcat gtccgagtcc gggccgctgc ggagctggat
     6841 gcagcccttc aggaaggcct cgaggtaggc gccctgcgcg aagtggcccg tccgcgagct
     6901 gccggtgacc ccgccgggcc ggtacacgcg caccgggaag ccgcgctcgg ccgcggcctg
     6961 agcggtgcgc tccgcgaccc acttcgactg cgtgtagccg ccgagcagcc agccgtcgtg
     7021 ctcgagcgag aagtcctccc cggcctcggg caggccccag aacggcagga cggcgaccgt
     7081 cgagatgtgg tggaccggca gcgagcgctc accgcccgct gagagctcga ggacgctgcg
     7141 gatgccggcg acgttcgtcc tcttgatgtc gtcgtagggc ttcttccagt tcagcgaggc
     7201 cgcgccgtgg tagatgccgt cgagctcgcc ctggaggcgc tcgtggtcgg cgtcggcgat
     7261 gccgagccgc ggctgggtca cgtcgcccac caccggctcg atgcggctcg ggtcatccgg
     7321 cgtcgggaga ccgaaccggc cgaggttgtc gatcacgcgg cggcgggccg cgtccgcgtc
     7381 gtcggcccgg acgaggcagt ggatcgtggc ggcggtgtcg cgcagcagac cgtggaccat
     7441 gaaggcaccg agaaagcccg tgccgccggt cacgaggacg ttgcgtgcgt cgggcagcgc
     7501 cgcgtcgccg gcgccgcccg cgggcttcag cgccggctcg agctgggcct cgggaagcat
     7561 cgcgacggtc gtcgtcgcga gcgggaccgg cttgctctcg gctgcttcgg tggtcatggc
     7621 tcgggggcgc acgcggggtg ggcccggcgc ggcggatcct attcgagggc cgcacgcgcg
     7681 ctctaagatg ccgcgccgtg gggggtccga tcgcgcgccg gccgtggcgc ttcctggtcg
     7741 tcgcactcgt cctgacgctc gtggccggtg cactcggagt gggcgtcgcc gacgtgctcc
     7801 agccctacaa cggcgatccc gatccgggcg ccgagagcgc ggcggccgcg gaccagatcc
     7861 gcgaggccac cggcgtggac ccgcgcggcg gcatcgtcgc gctagtcgag ccgggcggcg
     7921 gggcgcgctc accgagcggg cgcgccgcgg tcgcgcgcgt gcgcgcggag ctcgaggcgg
     7981 agcgaaccgt gggccgcgtc gtgtccccca ccacccccgg cggcacgccg ctggcctcgc
     8041 gggacgggcg tgcgacctac ctcgtcgcgt tcctgcgccc gggccaggcc gccgcgcacc
     8101 gcgacgccgc cgagcgcatc gaggagcgct tcgagggccg ccgcgacgta cgcatcggcg
     8161 gcgtcggcgc cggctacgtg gagggctccg agatcgcgga ggaggatctc gcgcgcgccg
     8221 agcagctcgt gttcccgctg ctcctcgccg cggcgttcct gatcttccgc ggcggcgtcg
     8281 cggcgatgct gccccttttc gtcggcttcc tgtcggtgct ctggacgctc gcgctgctgc
     8341 gcgtgctcgg cgaggccacc gacgtgtcgg tcttcgcgct caacctcgtc acgtcgctgg
     8401 cgctcggcct ctcgatcgac ttcagcctcc tgctcgtctc gcgcttccgc gaggagctcg
     8461 aacgcgcggg cggcgacgag cgcgcggtcg ccctccagcg cacgctcgcc acggcgggcc
     8521 gcacggtcct cttcagcagc ctcacgatcg cggcggcgct ggcgtcgctg ctcgtcttcc
     8581 cgcagcggct cctgtactcg ctcggggtgg ccggcctgct tgcggcgctg atgaccgcca
     8641 cgctggcgct ggttgtcgtg ccggcctgcc tggcgctgct cgggccacgc gtgaacgccc
     8701 tcgcgccgcg ccgcctgcag cgcgccgccg agcgcgaggc gcgccccgcc gaggccggct
     8761 tctggtaccg gaccgcgcag ctcgtcgcgc gcaggccgct gccggtcgcg cttgccgcgg
     8821 cggcgctcat gctggcgctg gccgccccgg tgctcggcat gcgcacgacc tccgccgaca
     8881 cccccgcgga gctgccggag ggagccacca cccgcgaggt ggacgacgac ctgcgcaccc
     8941 gcttcacgcc ggccaacgcc agc
//
LOCUS       DASVPL010000217        10872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_142403, whole genome shotgun sequence.
ACCESSION   DASVPL010000217 DASVPL010000000
VERSION     DASVPL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10872
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142403"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1071)
                     /locus_tag="VF517_11675"
     CDS             complement(100..1071)
                     /locus_tag="VF517_11675"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HEX8053640.1"
                     /translation="MGDLGQVGPGTRGKHGPVGAAVRSAALIAAVLAALSLPATASAD
                     HSDANLPWPAALPPQEVSAAVQPRPVDNCRRATIRCVDDLLRRLRAQWKPLDRACDHR
                     ALWPFAYIQITKLIRADLARDVPRWFPHRKWFTYVVTDFSNRYFRAYADYHRGRADRV
                     PYSWRVTFQATAAGDYHAGQDILLASNAHTQWDLPHAYAKLGMRTPSGETRKPDHDGV
                     NEVNTRVFDDIEDEYARRYDPTFAWVDMKPSPIDELGSQEMVKGWREGAWRNAERLMN
                     ARTPEERSEVDRSIATTANAWADFIAAGTLPGHRATRDAHCRAFHAG"
     gene            complement(1071..1973)
                     /locus_tag="VF517_11680"
     CDS             complement(1071..1973)
                     /locus_tag="VF517_11680"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HEX8053641.1"
                     /translation="MRFWICVAVLCTLAFGGYAATAGGSSHPANVHWESFLPALPSSS
                     SAQPGPVPHCRKATSRCIDEQVKRMKRLQREMGCDHRGVFATTYLELTRQYRDDLKAG
                     MKNQLQDLKYLYREVAVFANVYFETFKRFKRGEDVPEAWRIAFETARSGEVNGAQDML
                     LGINAHVQNDMPFVIAALGIRARDGSSRKPDHDVVNDVLDRAYERVVAEVSARYDPFV
                     STTNAPWNPGDDVAGLEMVRVWRENVWRNAERLVNAASDEERQRISEDIKARASDWAR
                     GIATFQTPGDRQRRDMYCAAHLAG"
     gene            complement(2020..2928)
                     /locus_tag="VF517_11685"
     CDS             complement(2020..2928)
                     /locus_tag="VF517_11685"
                     /inference="COORDINATES: protein motif:HMM:NF025695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi-alpha family protein"
                     /protein_id="HEX8053642.1"
                     /translation="MTEAGGEFTRYSEPAYTDAEPEQPHDPGVLDVVPGLARIAGVAW
                     WRTTQWSVETSVRVGARVVRFAASGQAPADLFRESGTDLRVWLRRFVDVVDESAHAAA
                     GTSSNGTDPSHRANGSANGDVPLRERGAELLRRSADVHLDDDCHPAYERILEEMAPDE
                     ARILRHMCSEGPQAAVDVRTGGPIGMLNSQLVAPGLTMVAREAGCRRPERVAAYLNNL
                     HRLGLLWFSRETLEDQATYQVLEAQPEVADAMTRAGRARTVRRSIHLTPFGEDFCKAV
                     LPLNTSEIDALPRPPSDDEPIDAELA"
     gene            complement(2933..4210)
                     /locus_tag="VF517_11690"
     CDS             complement(2933..4210)
                     /locus_tag="VF517_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053643.1"
                     /translation="MDSVWLHFITIPIFSGIIGYVTNWSGVIMLFYPIRFRGIRLPGL
                     APLVHLMPRKIQQIPGFMVGGLGWQGIIPSRAAKMGSIAVDKGIAKLGSAADFYKQLD
                     PEKMADHVLVSARRDMRDTVERIIQRENPDLWRDLPPGFRERLHERVQEQLPEIVHEV
                     VTEEIAENIDQLLDVKLMVIRRMEENPALANKVYTEVGSRELRLIINFGFLFGFLLGF
                     PVAAISELLIHGWWVLPILGIFVGYITNLIAIHMIFEPVEPKTYLGVIPFQGLFLRRQ
                     QEAGDIYARVIAEDIVTLRNIGEELMHGPRADRTRQMIEDALRPAVDRAVGRARPAVR
                     VAVGAREYDAIRDSVAAEGAEYTMTPLVDEEFGREQGERVRELISERIREMPPADFSE
                     MLRSAMREDEWLLYLHGAVLGFAGGLAHLAVFG"
     gene            complement(4278..6725)
                     /locus_tag="VF517_11695"
     CDS             complement(4278..6725)
                     /locus_tag="VF517_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8053644.1"
                     /translation="MARVRRTGIGAIRSAEVDRALKLCGGGIEAGHGAIVFLAGPPAS
                     GRSGLLAQLDLELRYEAARLRTVRGTVADGRYLPEQETRDGQQALALAQGALGLAGAA
                     LPILGLVGQVMSVSRTALALVEEGRLGSGEPTFDALFQLLRVAAREKPIVCLVDDCDE
                     ADGRWWSELLLGLAAEIERDLPLFLFLAVEGGAEPGANSDDEPDAMFAARHLTGRGLA
                     QWWPLGDATPDDLRVLTGAAEPDTTARLGELTDGRVGWAAELWAEWRTLGVVERDTDL
                     GPWRFAPGQPDRTASSIAAVVRRRLGQEFGDDSDGARLAWDVLACAALEGRYFTGQVV
                     AAALGLDSDAVVDLIDDRLLRDEHRPHGLVEEAGWLTVEDAPGEERHLARYRFASGLL
                     RSALVRYGLGQSEREARARGVADAIERVLGSAATALSGRMAALMSIAGDAELAAEWRR
                     RADGHMPADAIAEQARALFELEPESTDRATCRRACHILLQASFSYWAAGALNDVRTAG
                     QRAAILARHEGDEHAEAAALDTQGAAESYMGMPDEARTHLDAAAALFRAHGDRRGIAA
                     VALNQAYLEGNTGALDQARAHFDEAIALFHGLGDAHGEMNARMVKANLLIQHRDPDGA
                     AAALAPAIPLVEAYGDLSDQAKLREALGGAAYHARRFDQAIAYYREANQLYQEGGYPR
                     GMATSLRSYGISLASDGQWEEAESVLREVIVEAQAMENASGETEARAGLAHVLGATGR
                     AEAAIEEQRLVLELLRGSGMEHLRADRRVDLAKLMLAGGDVDGARAELEGALEDARRA
                     GAEPVVATATELLDSLP"
     gene            6793..7446
                     /locus_tag="VF517_11700"
     CDS             6793..7446
                     /locus_tag="VF517_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX8053645.1"
                     /translation="MPESTEQVRERYLAAILEPDPAAAREVVREAVRGGLAVEDAYGG
                     VLGAAMSEVGRRWEAGEISVAHEHLASEVTGSLVSELAGRIRAEPETGRLALICCSPG
                     ERHCIGGQMLAGLLEAAGWEVLYMGASLPGEDLVALADEEAADVIALSTTMTNHLDAV
                     AETIRAVQALEEPPLVIVGGQAYGGEDDAQRVGADAYAASVAEAPALLRRRLPPAAA"
     gene            complement(7665..9083)
                     /locus_tag="VF517_11705"
     CDS             complement(7665..9083)
                     /locus_tag="VF517_11705"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8053646.1"
                     /translation="MISALLAGVIERAPVSFPMIFLTLGLALGGGSLGVIEPDLEGDA
                     LRTVAFATLALVLFLEAMSLDLRHLRRYWVVPALTLGPGSLIVVVLVAVTAMLVAGLD
                     PVLAFIVGAILASTDPVVLRDVVHDERIPSSVRRALSLEAGMNDVIVLPTILILTAVA
                     ASDVGGTLDWARFLAELLVVGPALGALVGWAGAALMAEVDQRRPVRSEWQALYGVGLV
                     LCAFAVGETGGEDGFLAAFAAGAAVSVSNRRMCDCFLDFGEVIAELLMLLSFVLFGAV
                     LSTEIGNVPLADGLLLAAVALLVIRPLAVGGLLTLRTATLSNHGRAFIAWFGPRGLNS
                     LLLALIVIEAGVPEGREVFALVGIVVLASTFLHGITATPMAAWYGRRVDAATHPEERE
                     SSMDGLLGPLSADDVPRIEAEELAKLLESAEPPVVLDVRSRSGYANDPFQIPGAARVR
                     PDEVSVWAEENERDKLVVLYCT"
     gene            complement(9192..10649)
                     /locus_tag="VF517_11710"
     CDS             complement(9192..10649)
                     /locus_tag="VF517_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8053647.1"
                     /translation="MTAVAVEASTRPLTERSGLELAAAIRAGEVSSRDAVEAHIEQIE
                     RVNPRINAVVVERFAEARAEAAAADERIAAAGPDEELPPLLGVPCTIKESLSLAGLPQ
                     SAGVVARADRPCERSSTVVERILAAGAIPLGLTNTSELTLWVETENRLYGRTRNPYDS
                     SRTAGGSSGGEGAAIGSGASPLGLGTDFAGSIRLPAFFNGVFGHKPSVGLVPMTGHYP
                     AAGGASAPFAVAGPLARRAEDLIVVLRAIAGPDGEDELAEDMELGDPADVSLDGLRVV
                     VSESGTLGPTGPELAQARERAAGALAAAGARLERVSTKSMRRALEVYMATVATTGITL
                     ASLLEDAGAEPLTVRSALRRGGPHTVATRLTAFLERATARVPDRRTQKAVAARDAFVR
                     ELCDTIGDGVLLHPPAPKVAPRHGGTIGRVWWIHPTLVFNLAGLPVTQVPLGLGNRGL
                     PLGVQVVAGPGRDHVAIAAALELERVFGGWTPPPA"
     gene            10667..>10872
                     /locus_tag="VF517_11715"
     CDS             10667..>10872
                     /locus_tag="VF517_11715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053648.1"
                     /translation="MRRLLLLVCAIVLVDTMFYAAITPLLPHYSDELGLSKAEAGVLA
                     AAYPAGTLLGSLPSGWLAVRIGAKR"
BASE COUNT         1452 a         4053 c         3936 g         1431 t
ORIGIN      
        1 gacgcctcga accgccccac tccccggccg ccgacggccg cccgccgcgg agccgcagcg
       61 gccgtgggcg ccagccgcag ggcacgccgg gcgcgggccc tagcccgcgt ggaaggcgcg
      121 gcagtgcgcg tcgcgcgtgg cgcggtggcc cggcagcgtg ccggctgcga tgaagtcggc
      181 ccaggcgttc gcggtggtcg cgatcgagcg atccacctcg ctgcgctcct cgggcgtgcg
      241 cgcgttcatc aggcgctcgg cgttgcgcca ggcgccctcg cgccagccct tgaccatctc
      301 ctgactgccc agctcgtcga tcggcgaggg cttcatgtcg acccacgcga acgtcgggtc
      361 gtagcggcgc gcgtactcgt cctcgatgtc gtcgaacacg cgcgtgttca cctcgttcac
      421 gccgtcgtgg tccggcttgc gcgtctcgcc ggacggggtg cgcatgccca gcttcgcgta
      481 cgcgtgcggg aggtcccact gcgtgtgggc gttgctcgcg agcaggatgt cctggccggc
      541 gtggtagtcg cccgcggccg tcgcctggaa ggtcacccgc cacgagtacg gcacgcggtc
      601 ggcgcgcccg cggtggtagt ccgcgtacgc gcggaagtag cggttcgaga agtccgtgac
      661 gacgtacgtg aaccacttgc ggtgcgggaa ccagcgcggc acgtcgcggg cgaggtcggc
      721 gcggatcagc ttcgtgatct ggatgtacgc gaacggccac agcgcccggt ggtcgcaggc
      781 gcggtcgagc ggcttccact gcgcgcgcag gcggcggagc aggtcgtcga cgcagcggat
      841 cgtcgccctg cggcagttgt cgaccggtcg cggctgcacc gccgcgctga cctcctgcgg
      901 cgggagcgcg gcgggccagg ggaggttcgc gtcgctgtgg tcggcgctcg cggtggcggg
      961 caggctcagc gccgccagaa cggcggcaat cagggcggcg gaccggaccg ccgcgcctac
     1021 tgggccatgt ttgccccgcg taccggggcc aacttggccc aggtcgccca tcaccccgcg
     1081 aggtgcgcgg cgcagtacat atcgcggcgc tggcggtcgc cgggcgtctg gaacgtcgcg
     1141 atgccgcgtg cccagtccga cgcgcgcgcc ttgatgtcct cggagattcg ctggcgctcc
     1201 tcgtccgagg cggcgttcac gagccgctcg gcgttgcgcc acacgttctc gcgccagacc
     1261 ctcaccatct cgagcccggc cacgtcgtcg ccggggttcc agggtgcgtt cgtggtcgat
     1321 acgaacgggt cgtagcgcgc cgacacctcg gcgactacgc gctcgtacgc gcggtcgagc
     1381 acgtcgttca ccacgtcgtg gtcgggcttg cgcgacgagc cgtccctcgc gcggattccg
     1441 agggccgcga tcacgaacgg catgtcgttc tgcacgtgcg cgttgatccc gagcagcatg
     1501 tcctgcgcgc cgttgacctc gccgctgcgc gcggtctcga aagcgatccg ccaggcctcc
     1561 gggacgtcct cgccgcgctt gaagcgcttg aacgtctcga agtacacgtt cgcgaagacg
     1621 gcgacctcgc gatacaggta cttgaggtcc tggagctggt tcttcatgcc ggccttcagg
     1681 tcgtcgcggt actggcgcgt cagctcgagg taggtggtcg cgaacacgcc gcggtggtcg
     1741 cagcccatct cccgctgaag gcgcttcatc cgcttgacct gctcgtcgat gcagcgcgag
     1801 gtggccttgc ggcagtgcgg caccggcccc ggctgggcgc tcgacgagct cggcagcgcg
     1861 gggaggaagg actcccagtg cacgttcgcg ggatgggagg aaccgccggc ggtggcggcg
     1921 taccccccga acgcgagcgt gcagagcacg gccacgcaga tccaaaagcg catcgcctca
     1981 accattcccc gcgtggcggg cgggaaacgc gccgtttcgc taggccagct cggcgtcgat
     2041 cggctcgtcg tcggacgggg gccgcgggag cgcgtcgatc tcggaggtgt tgagtggcag
     2101 gacggccttg cagaagtcct cgccgaaagg cgtcaggtgg atgctgcgcc ggacggtccg
     2161 ggcgcggccc gcgcgggtca tcgcgtcggc gacctcgggc tgggcctcga gcacctggta
     2221 ggtggcctgg tcctcgaggg tctcgcggga gaaccagagc aggccgagcc ggtgcaggtt
     2281 gttcaggtag gccgcgacac gctcgggccg ccggcagccc gcctcgcggg cgaccatggt
     2341 gagccccggc gcgacgagct gggagttgag catcccgatc gggccgccgg tgcgcacgtc
     2401 caccgccgcc tgcggcccct cgctgcacat gtgccggagg atgcgcgcct cgtcgggtgc
     2461 catctcctcg aggatgcgct cgtacgcggg gtggcagtcg tcgtcgaggt gcacgtcggc
     2521 cgagcggcgc agaagctcgg cgccgcgctc gcgcagcggc acgtcgccgt tcgcgctccc
     2581 gttcgcgcgg tgggacgggt cggtcccgtt cgagctcgtc ccggcggccg cgtgagcgct
     2641 ctcgtccacc acgtccacga agcgccgcag ccagacgcgc agatcggtgc cgctctcgcg
     2701 gaacaggtcg gcgggcgcct gcccggacgc ggcgaagcga accacccgcg cgcccacgcg
     2761 cacggaggtc tcgacgctcc actgcgtggt ccgccaccac gccacgccgg cgatccgcgc
     2821 cagcccgggc acgacgtcga ggaccccggg gtcgtggggc tgctcgggct cggcgtccgt
     2881 gtaagccggc tcgctgtagc gcgtgaactc gccgccggcc tcggtcacgc gatcacccga
     2941 acaccgccag atgcgcgagc ccgcccgcga agccgagcac cgcgccgtgc aggtacagca
     3001 gccactcgtc ctccctcatc gcactgcgaa gcatctcaga gaagtccgcc ggcggcatct
     3061 cgcggatgcg ctccgagatc agctcgcgca cgcgctcgcc ctgctcgcgt ccgaactcct
     3121 cgtcgaccag cggggtcatc gtgtactcgg cgccctcggc ggcgaccgag tcgcggatcg
     3181 cgtcgtactc gcgcgcgccc acggccacgc ggaccgccgg ccgggcgcgg cccacggcgc
     3241 ggtcgacggc gggtcgcagg gcgtcctcga tcatctggcg cgtccggtcc gcccgcggcc
     3301 cgtgcatcag ctcctcgccg atgttcctca gcgtgacgat gtcctcggcg atgaccctgg
     3361 cgtagatgtc gccggcctcc tgctggcgcc gcaggaacag gccctggaat gggatcacgc
     3421 cgaggtacgt cttgggctcg accggctcga agatcatgtg gatggcgatc aggttggtga
     3481 tgtagccgac gaagatcccg aggatcggca ggacccacca gccgtggatc agaagctcgc
     3541 tgatcgcggc caccgggaag cccagcagga agccgaacag gaacccgaag ttgatgatca
     3601 gccgcagctc gcgcgagccg acctccgtgt agaccttgtt cgccagcgcc gggttctcct
     3661 ccatgcgccg gatgaccatg agcttgacgt cgaggagctg gtcgatgttc tccgcgatct
     3721 cctcggtcac gacctcgtgg acgatctccg ggagctgctc ctgcacgcgc tcgtggaggc
     3781 gctcgcggaa gccgggcggg aggtcgcgcc agaggtcggg gttctcgcgc tggatgatcc
     3841 gctcgacggt gtcgcgcatg tcgcggcgcg cgctgaccag cacgtggtcg gccatcttct
     3901 ccgggtcgag ctgcttgtag aagtcggcgg cgctgccgag cttcgcgatc cccttgtcga
     3961 ccgcgatgct gcccatcttc gccgcacgcg aggggatgat gccctgccag ccgagcccgc
     4021 cgaccatgaa gccggggatc tgctggatct tgcgcggcat caggtgcacg agcggggcga
     4081 gcccgggcag ccggatcccc ctgaagcgga tcggatagaa gagcatgatc acgccgctcc
     4141 agttcgtgac gtagccgatg atccccgaga agatcgggat cgttatgaag tgcagccaga
     4201 cgctgtccaa gtttcccccg ccaggcattc tcccggagcg gggccggtag cggcggcagg
     4261 cgggcgcggg cttgcgttca ggggagggag tcgagcagct ccgtggcggt ggcgacgacg
     4321 ggttcagcgc cggcgcgccg cgcgtcctcg agagcaccct cgagctcggc tcgcgcgccg
     4381 tccacgtcgc ccccggcgag catgagcttc gccaggtcca cgcggcggtc cgcgcgcagg
     4441 tgctccatcc cgcttccgcg cagcagctcg agcaccagcc gctgctcctc gatggcagcc
     4501 tccgctcgcc cggtcgcgcc gagtacgtgc gccaggccgg cccgcgcctc cgtctcgccg
     4561 gacgcgttct ccatggcctg cgcctcgacg atcacctcgc gcagcacgct ctcggcctcc
     4621 tcccactgac cgtcggacgc cagggagatg ccgtagctgc gcagcgacgt ggccatgccg
     4681 cgcgggtaac cgccctcctg gtagagctgg ttcgcctccc ggtagtacgc gatcgcctgg
     4741 tcgaaccggc gcgcgtggta ggccgcgccg ccgagcgcct cgcgcagctt ggcctgatcg
     4801 ctcaggtcgc catacgcctc gaccagcggg atcgcgggcg ccagggccgc ggccgccccg
     4861 tccgggtcgc gatgctgaat gagcaggttc gccttgacca tccgtgcgtt catctcgcca
     4921 tgcgcgtcgc cgaggccgtg gaacagggcg atcgcctcgt cgaagtgcgc gcgcgcctgg
     4981 tcgagcgcac cggtgttgcc ctcgaggtag gcctggttca gcgccaccgc cgcgatcccc
     5041 cgccggtcgc cgtgtgcacg aaagagcgcg gcggcggcgt ccaggtgcgt cctcgcctcg
     5101 tccggcatgc ccatgtagga ctcggcggcg ccctgcgtgt cgagcgcggc ggcctcagcg
     5161 tgctcgtcgc cctcgtggcg cgccaggatc gcggcgcgct gccctgcggt tcgcacgtcg
     5221 ttcagcgctc ccgcggccca gtagctgaag ctcgcctgga gcaggatgtg gcaggcgcgg
     5281 cggcaggtag cgcgatccgt gctctcgggc tcgagctcga acagcgcgcg cgcctgctcg
     5341 gcgatcgcgt cggccggcat gtgcccgtcc gcccggcgcc tccactccgc ggcgagctcg
     5401 gcgtcgcccg cgatcgacat gagcgccgcc atccggcccg acagcgccgt cgcggccgag
     5461 ccgagcaccc gctcgatcgc gtccgccacc ccgcgggcgc gcgcctcgcg ctcgctctga
     5521 ccgaggccgt agcgcaccag ggccgagcgc agcaggccgg acgcgaaccg gtagcgcgcg
     5581 aggtgccgct cctcccccgg cgcgtcctcc accgtcaacc agccggcctc ctcgaccagc
     5641 ccgtgcgggc ggtgctcgtc gcgcaggagg cggtcgtcga tcaggtcgac caccgcgtcc
     5701 gagtcgagcc cgagcgcggc ggccaccacc tggccggtga agtagcggcc ctcgagcgcc
     5761 gcgcaggcga gcacgtccca cgcgagccgc gcgccgtccg agtcgtcgcc gaactcctgc
     5821 ccgagccgac ggcgcacgac cgccgcgatc gagctcgccg tgcggtcggg ctggccgggc
     5881 gcgaaccgcc acgggccgag gtcggtgtcg cgctcgacca cgccaagcgt gcgccactcc
     5941 gcccacagct cggccgccca gcccacgcga ccgtcggtca gctcccccag ccgcgcggtc
     6001 gtgtcgggct cggcggcacc ggtgagcacg cgcaggtcgt ccggggtggc gtcgccgagc
     6061 ggccaccatt gcgcgagccc gcggccggtg aggtggcgcg cggcgaacat ggcgtcgggc
     6121 tcgtcgtcgc tgttcgcccc cggctccgcg ccgccctcca ccgccaggaa caggaacagc
     6181 gggaggtcgc gctcgatctc agcggccagc ccgagcagca gctcagacca ccagcgcccg
     6241 tcggcctcgt cgcagtcgtc cacgaggcag acgatcggct tctcgcgcgc cgccacacgc
     6301 aggagctgga acagggcatc gaacgtcggc tccccggagc ccagccggcc ctcctccacg
     6361 agcgcgagcg cggtgcgcga gacgctcatc acctgcccga cgagaccgag gatcggcagc
     6421 gccgcgccgg caagcccgag cgccccctgg gcgagtgcga gcgcctgctg gccgtcacgc
     6481 gtctcctgct ccggcaggta gcgcccgtcc gcgaccgtcc cgcgcacggt gcgcagccgc
     6541 gccgcctcgt agcgcagctc gaggtcgagc tgcgcgagca atccggaccg cccgctcgcc
     6601 ggcggccccg ccaagaacac gatcgccccg tgcccggcct cgatgccgcc gccgcagagc
     6661 ttcagggcgc gatcgacctc cgcggaccgg atcgcgccga tgccggttct cctgacgcga
     6721 gccacggcgc cgagtctcgc gccgggagcc gctgcattca acccccgaga gcgggtagct
     6781 tccgcagcgc cggtgccgga gtcaaccgaa caagtacgcg agcgctacct cgcggcgatc
     6841 ctcgagccgg accccgccgc ggcgcgcgag gtggtgcgcg aggccgtccg cggcgggctc
     6901 gcggtcgagg acgcgtacgg cggcgtgctc ggggccgcga tgagcgaggt cgggcgccgc
     6961 tgggaggccg gtgagatcag cgtcgcgcac gagcacctcg cgtcggaggt gaccgggtcg
     7021 ctggtctcgg agctcgcggg ccggatcagg gcagagcccg agaccggacg cctcgcgctg
     7081 atctgctgct cgcccgggga gcgtcactgc atcggcgggc agatgctcgc ggggctcctg
     7141 gaggcggccg gctgggaggt gctctacatg ggcgcgtcgc tgcccggcga ggacctcgtc
     7201 gcgctggccg acgaggaggc cgccgacgtg atcgcgctct ccacgacgat gaccaaccac
     7261 ctcgacgcgg tggccgagac gatccgcgcc gtacaggcgc tcgaggagcc gccgctcgtg
     7321 atcgtcggcg ggcaggccta cggcggagag gacgacgctc agcgggtggg cgccgacgcc
     7381 tacgccgcga gcgtggccga agcgcccgcg ctgctgcggc ggcggctgcc gcccgcggcg
     7441 gcgtaaggcg ccgcgggccg cgcgagccgc ggcccgagtc ccacgccccc gccgcgcatc
     7501 cccgccggcc ggcggcgcgc ctacgccgcc gcccccgcct ccacgcccgc gcgctcgacc
     7561 tcgtgctcct cgcgccaggc atcgagcccg ccgcgcagcg ccgccgccct gaacccctcg
     7621 ttgatcagct gccgcgccgc ccgggcgctg gttccctcgt tgagtcacgt gcagtagagg
     7681 acgaccagct tgtcgcgctc gttctcctcc gcccagacgg agacctcgtc gggccgcacg
     7741 cgcgcggcgc ccgggatctg gaacgggtcg ttcgcgtatc cggagcggga gcgcacgtcg
     7801 agcacgaccg gcggctcggc cgattcgagc agcttggcca gctcctcggc ctcgatgcgc
     7861 ggcacgtcgt cggccgacag cggcccgagc aggccgtcca tcgagctctc gcgctcctcg
     7921 ggatgcgtgg cggcgtccac ccggcggccg taccacgccg ccatcggcgt ggcggtgatc
     7981 ccgtgcagga aggtcgatgc gagcacgacg atcccgacca gcgcgaacac ctcgcgcccc
     8041 tcgggcaccc cggcctcgat cacgatcagc gcgagcagca gcgagttgag cccgcgcggc
     8101 ccgaaccacg cgatgaacgc gcggccgtgg ttgctcagcg tggccgtgcg cagcgtgagc
     8161 agaccgccga ccgcgagcgg gcggatcacg agcagggcga ccgccgcgag cagcagcccg
     8221 tcggcgagcg gcacgtttcc gatctcggtc gagagcacgg cgccgaacag cacgaacgag
     8281 agcagcatca gcagctcggc gatcacctcg ccgaagtcga ggaagcagtc gcacatccgc
     8341 cggttcgaga ccgacacggc cgcgcccgcc gcgaacgccg cgaggaaccc gtcctcgccc
     8401 ccggtctccc cgacggcgaa cgcgcacagc acgagcccca ccccgtagag cgcctgccac
     8461 tcgctgcgca ccgggcgccg ctggtccacc tccgccatca gcgcggcgcc cgcccagccc
     8521 acgagcgcac cgagcgccgg cccgacgacc agcagctcgg cgaggaagcg cgcccagtcg
     8581 agcgtgccgc ccacgtcgct cgccgcgacg gcggtgagga tgaggatcgt gggcagcacg
     8641 atcacgtcgt tcatcccggc ctcgaggctc agcgcccgcc ggacggagga ggggatccgc
     8701 tcgtcgtgga cgacgtcgcg caggaccacc gggtcggtgg aggcgaggat cgcgccgacg
     8761 atgaacgcga gcaccgggtc gaggccggcc accagcatcg ccgtcacggc caccagcacg
     8821 acgacgatca gcgacccggg gccgagggtg agcgcgggca ccacccagta gcggcgcagg
     8881 tggcgcaggt cgagcgacat cgcctcgagg aacaggacca gcgcgagggt cgcgaacgcc
     8941 acggtgcgca gcgcgtcgcc ctcgaggtcg ggctcgatca cgcccagcga cccgccgccg
     9001 agcgccagcc cgagggtcag gaagatcatc gggaacgaga ccggcgcgcg ctcgatgacg
     9061 cccgcgagga gcgccgagat catgagcaca ccggctccga gagcgaagag gccgaccgga
     9121 tccaagtcgc ggcgacgata ccgccggctg gcgctgctac cggcgcatgg cgaaggggtc
     9181 gatcggcggg atcaggcggg cggcggcgtc cagccgccga acactcgctc gagctccagg
     9241 gcggccgcga tcgccacgtg gtcgcggccc gggccggcga ccacctgtac tccgagcggc
     9301 aggccgcggt tgccgaggcc gagcggcacc tgggtgaccg ggaggccggc gaggttgaac
     9361 accagcgtcg gatggatcca ccacacccgc ccgatcgtgc cgccgtggcg cggcgcgacc
     9421 ttcggagccg gcgggtggag cagcacgccg tcgccgatcg tgtcgcacag ctcgcggacg
     9481 aaggcgtcgc gcgccgcgac cgccttctgg gtgcgccggt caggcacgcg ggcagtggcg
     9541 cgctccagga acgccgtgag cctggtcgcg acggtgtgcg gcccgccccg ccgcagcgcg
     9601 ctgcgcaccg tcaggggctc ggcgccggcg tcctcgagca gggaggccag tgtgatcccg
     9661 gtggtggcca ccgtggccat gtagacctcg agcgcgcgcc gcatgctctt ggtggagacg
     9721 cgctcgaggc gcgcgcccgc ggccgccagc gccccggcgg cgcgctcacg cgcctgggcc
     9781 agctccggcc cggtcgggcc gagcgtgccc gactcgctca ccaccactcg caggccgtcg
     9841 aggctcacgt ccgccggatc gcccagctcc atgtcctcgg ccagctcgtc ctcgccgtcg
     9901 ggtccggcga tcgcccggag cacgacgatc agatcctcgg cgcgccgcgc gagcggcccg
     9961 gccaccgcga acggcgcgct cgctcccccc gccgcggggt agtggccggt catcggcacg
    10021 agccccacgg acggcttgtg gccgaagacc ccgttgaaga acgccggcag gcggatcgag
    10081 ccggcgaagt cggtgccgag cccgagcggc gatgcccccg agccgatcgc ggcgccctcg
    10141 ccgcccgacg agccgccggc ggttcggctg gagtcgtatg ggttgcgggt gcgtccgtag
    10201 aggcggttct ccgtctccac ccagagggtc agctcggagg tgttggtgag cccgagcggg
    10261 atggccccgg cggcgaggat gcgttcgacg acggtcgagg accgctcgca gggacgatcg
    10321 gctcgcgcca ccacccccgc ggactgcggc aggccggcca ggctgagcga ctccttgatc
    10381 gtgcacggca ctccgagcag cggcggcagc tcctcgtccg gccccgcggc ggcgatccgc
    10441 tcgtcggccg cagcggcctc ggcgcgcgcc tcggcgaagc gctcgaccac gacggcgttg
    10501 atccgcggat tcacgcgctc gatctgctcg atgtgcgcct cgaccgcgtc gcgcgagctc
    10561 acctcacccg cgcggatcgc agccgccagc tcgaggcccg agcgctcggt gagcgggcgg
    10621 gtcgaagcct cgactgcgac agcggtcata tacacatcat cccccgatgc gccgcctgct
    10681 gctcctggtg tgcgcgatcg tcctcgtgga cacgatgttc tacgcggcga tcacgccgct
    10741 gctgccccac tactcggacg agctcggcct gtcgaaggcc gaggccggcg tgctcgcggc
    10801 ggcgtacccg gcgggcaccc tgctcggctc gctgccgagc ggctggctgg cggtgcggat
    10861 cggcgcgaag cg
//
LOCUS       DASVPL010000218         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_380032, whole genome shotgun sequence.
ACCESSION   DASVPL010000218 DASVPL010000000
VERSION     DASVPL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380032"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VF517_11720"
     CDS             <1..1185
                     /locus_tag="VF517_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8053649.1"
                     /translation="AAVYLTLGDEPGDVSHPDVEFTETEDAPKAEEPKDLTWPVYGLT
                     PERTRYLNAPEVDPPFKKLWRLRIGHLIEFQPVLAAGVLYVVPNDGVARAVDAETGKV
                     KWSTRVGTLNASSPAYYKGDLFIATLSKRITALRAKDGRQRWKRNLPSRTESSPVVIG
                     GVVYLGSEDGRVYALKAKTGRILWTYKARGAVKAGLAYSEGRLFFGDYNGQVTALRAK
                     DGSRIWRTGTEGRSFGRSGNFYSTPAVKYGRVYIGNTDGFVYSFAARNGQLAWRHRTG
                     AFVYAAPAVARVEGTPPTVYIGSYDKHFYALDARSGKVRWKFNAKGRISGAPTVVGRV
                     VYFSELENKTTSGLDVRTGRRVFKLSSGAFNPAISDGRRLFVTGYSSLFGFEPRKRSK
                     RK"
     gene            complement(1127..>1994)
                     /locus_tag="VF517_11725"
     CDS             complement(1127..>1994)
                     /locus_tag="VF517_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8053650.1"
                     /translation="QARELLWNQKVIESNQFESYFRVNSLFFDPNIYGRFLMVVMIGL
                     AAVLLWARSGRAVAVSGGLLALLWGGLVLTFSQSSFAALLVGLASLAALRWSFRRVAA
                     GIGLCAAVALVVAFGLGIGSDEIDESSGNRLDLIGGGLSMVADRPLHGFGSGSFGELY
                     REREEASSQRAASASHTIPITVSAEQGAIGLIAYLVLVVVSLRLLFAGLGPLSGRDPP
                     ARLVTRAFLAAAYAALLFHTLLYAAFLEDPIAWTLLAIGIVLLRPVAESGAATSSSSA
                     SSARTRTATSTP"
BASE COUNT          336 a          708 c          703 g          247 t
ORIGIN      
        1 gcggccgtct acctgaccct cggcgacgag ccgggggacg tctcgcaccc ggacgtcgag
       61 ttcaccgaga ccgaggacgc tccgaaggcg gaggagccca aggacctcac ctggccggtc
      121 tacggcctga cgcccgagcg cacccgctac ctgaacgcgc ccgaggtcga cccgccgttc
      181 aagaagctct ggcggctgcg catcggccac ctgatcgagt tccagccggt gctcgccgcc
      241 ggcgtgctgt acgtggtgcc gaacgacggc gtggcccggg cggtggacgc cgagaccggc
      301 aaggtgaagt ggtccacgcg cgtgggcacc ctgaacgcct cctccccggc ctactacaag
      361 ggcgacctct tcatcgcgac gctgtcgaag cggatcaccg cgctgcgcgc gaaggacggt
      421 cgccagcgct ggaagcgcaa cctgccgagc cgcaccgagt cgtcgccggt cgtgatcggg
      481 ggagtcgtgt acctgggctc cgaggacggc cgggtctacg cgctgaaggc gaagacgggc
      541 cgcatcctct ggacctacaa ggcgcgcggc gccgtgaagg cgggcctcgc ctactcggag
      601 gggcgcctgt tcttcggcga ctacaacggg caggtcacgg cgctgcgggc caaggacggc
      661 tcgcgcatct ggcggaccgg caccgagggg cgcagcttcg gccgctccgg caacttctac
      721 tcgaccccgg cggtgaagta cgggcgcgtc tacatcggga acacggacgg cttcgtctac
      781 tccttcgccg cgcgcaacgg ccagctcgca tggcgccacc gcaccggcgc gttcgtctac
      841 gcggctccgg ccgtggcgcg cgtcgagggc actccgccga cggtctacat cggctcctac
      901 gacaagcact tctacgcgct ggacgcccgc tccggcaagg tgcgctggaa gttcaacgcc
      961 aaggggcgca tctcgggggc gccgacggtg gtcgggcgcg tcgtctactt ctcggagctc
     1021 gagaacaaga ccacgagcgg gctcgacgtg cgcaccggga ggcgcgtctt caagctgtcg
     1081 agcggggcgt tcaacccggc gatctcggac ggccggcggc tattcgtcac ggggtactcg
     1141 tcgctgttcg ggttcgagcc gaggaagcgc tcgaagagga agtagccgcg ccgctctcgg
     1201 cgaccgggcg cagcagcacg atcccgatcg cgagcagggt ccacgcgatc gggtcctcca
     1261 ggaaggcggc gtagagcagc gtgtggaaga gcagcgccgc gtaggcggcc gccaggaacg
     1321 cgcgcgtgac gagccgggcg ggcgggtcgc gacctgagag cggcccgagg ccggcgaaca
     1381 gcagccgcag cgacacgacg acgagcacga ggtaggcgat caggccgatc gcaccctgct
     1441 cggctgagac cgtgatcggg atcgtgtgcg aggccgacgc ggcgcgctgc gagctggcct
     1501 cctcgcgctc gcggtacagc tccccgaagg agcccgagcc gaagccgtgc agcggccggt
     1561 cggccaccat cgacaggccg ccgccgatca ggtcgaggcg gttgccggac gactcgtcga
     1621 tctcatccga tccgatcccc agcccgaacg cgaccaccaa cgccaccgcc gcgcagagcc
     1681 cgatgcccgc ggccacgcgg cggaagctcc agcgcagcgc cgcgagcgac gcgaggccga
     1741 cgagcagcgc cgcgaagctc gactgcgaga acgtcagcac gagcccgccc cagagcagcg
     1801 cgagcaggcc gccggacacc gccacggccc ggccgctgcg cgcccacagc agcaccgccg
     1861 ccagtccgat catcaccacc atcaaaaagc gcccgtagat gttcgggtcg aagaacagcg
     1921 agttgacgcg gaagtagctc tcgaactggt tcgactcgat gaccttctgg ttccacagga
     1981 gctcgcgcgc ctgg
//
LOCUS       DASVPL010000219        15698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_25873, whole genome shotgun sequence.
ACCESSION   DASVPL010000219 DASVPL010000000
VERSION     DASVPL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15698
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25873"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VF517_11730"
     CDS             complement(<1..182)
                     /locus_tag="VF517_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053651.1"
                     /translation="MGGFGRGSRAIALAIALTFTMAGAASADVNQVAFDEYPVGTQIT
                     NQYESKGVTFADGANGT"
     gene            533..1741
                     /locus_tag="VF517_11735"
     CDS             533..1741
                     /locus_tag="VF517_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8053652.1"
                     /translation="MRFFEFESRQILAKEGIPLAGHGFATDAEQAKTIAAEIGKPVVI
                     KSQVLTGGRMKAGGVQFADTPEEAEGHARDILALEINGHMPKGVLIAEKAPIAQEYYA
                     GVIYDGVRKLPTFVFSDMGGIDIEEVAESHPEHIGRGHFSTILPNMDFRAKDVVAQVG
                     ITGSDLNRLTPILSKLAQLFVKYDMTLAEINPLAKLEDGTFVAVDAHMDMENEARPRQ
                     KAILKELGIGDEETREAREPTDFEIKGEQIDAVDHRGVAGNVTEFDGNIGLVIGAGGG
                     SLTLFDAVRNSGGSPANYCEIGGNPSVSKAKNLAKLVLEKPGVEKIAVMMSIVSNTRV
                     DIVARGVIAACLELGHDPAEKIAVFRIPGAWEEEGFKILEKYGVEYFDRTVSMHEAAR
                     RAVEKIGATA"
     gene            1738..2652
                     /locus_tag="VF517_11740"
     CDS             1738..2652
                     /locus_tag="VF517_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX8053653.1"
                     /translation="MNHLIDQDTTFIVQGITGREAVNLTRECLDYGSKIVGGVTPGRK
                     GREVHGVPVFDSVAQLVESIGSVPDGSVVTVPPAFTRDAVFEAIEAGVRLIVIVTERI
                     PRYDVAQMVELANLRGARIIGPNCLGIIRPGVAKMGGIGGPAKDAQKAYQPGSIGVMS
                     RSGGMTTEISSTLTAAGLGVSTAVSIGGDAIIGSTYAELMPLFEADEQTEGIVIYTEP
                     GGRMEAELAAWVTENESRLPIVAFMAGRFMDEMPGMSFGHAGTIVEGKADTATEKIAR
                     LEAAGITVAEDIASIPDLMKEKLGKVAA"
     gene            2652..2888
                     /locus_tag="VF517_11745"
     CDS             2652..2888
                     /locus_tag="VF517_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053654.1"
                     /translation="MAGVFIDVELDDGIRDDAELAKKLEDACPVDIYAARDGRVEVVE
                     ENLDECILCELCVKTAPAGKLRVRKLYDGTALER"
     gene            complement(2891..3745)
                     /locus_tag="VF517_11750"
     CDS             complement(2891..3745)
                     /locus_tag="VF517_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8053655.1"
                     /translation="MTGDSNETRACPACAADGATPAGEENGWALARCGRCGVRFTVEA
                     PTDEHLQEIYNRIYSEGDAYQMHLDQIRELEAKGKRKPGLYRSLIFLNRYKPKPDDRL
                     LEVGCGVGLFLSAANRKGWQVEGIDLSETALQASRDVHHLPVRQGRFDELDFDEGAYS
                     VIATFEVLEHLTDPRGFLEKAKRLLKPGGLLVCSVPNEGAKVPFPPVRGQASTPPVHL
                     NFWDRAALTRFFELNGYRVERMITQRTMNSVVHPREDAKRFLRLQAGAAFGRYEGIHL
                     FAAARPAG"
     gene            3801..5288
                     /locus_tag="VF517_11755"
     CDS             3801..5288
                     /locus_tag="VF517_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8053656.1"
                     /translation="MTEEAQGAGAGRNTLLALMTQLVTGLFTAVLTIYLVRALGPREF
                     GVLSLAIGIGTVLLLPSDFGISGSASRFIAERFPDRPAIAAIMADALRLKLAIGAIVS
                     IALLATAGLIADAYGEPELGWPIRWMAIAVFGQSMVGFFRYAFLSMRDAGTGFRIVAG
                     ESAVEAAATIGLVVLAGGAASAAAGRAIGYTFGAILAGSLAARLLGRAAFSRSRRLAD
                     ARRRLARYAGALFAIDVAFSASVQMAPLMIGGFLGAREVGLFTAPSRLMILLQYPGLA
                     LANGVGPRMARGEDHEPETRLFTTAFRYLIVLQALLLVPIVAWADPIVDLLLGSEFSE
                     SADLLRQLTPYIFSSGLAAILASGLTYLGEAKRRLPIAIGDVFLTAGLTAGGLATIGL
                     SGAAYAADIVSVLYVGVHFWIIRGLVDLPLRPLLLALVRGLLAAAAAAGVLVLFGTDD
                     LAVWEWVAGGAGALLAFTAVVVLTREVSLGELRSMVALVARRSRA"
     gene            5428..6195
                     /locus_tag="VF517_11760"
     CDS             5428..6195
                     /locus_tag="VF517_11760"
                     /inference="COORDINATES: protein motif:HMM:NF004850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX8053657.1"
                     /translation="MAGKPKSAEMHPGPGFRIREKINRPRPETIAALGAFDAPAISDL
                     MNRLYAMTPAIKNLTDPNLRLLGPATTVKCYPGDNLMVHKALDIAEPGDVIVVDTSGQ
                     QNTAVLGDLVSTKARHRGVAGFLVDGLVRDIAGIRMLGDFPVYARDVTPIGPLHRGPG
                     EINFPISAGGIVVQPGDAIVGDSNGVVVVPQDIVEEMVRRLTAQQAAESDYTAAVARG
                     EFNNSWVDKTLTDAGVPLVNGNGNHKAPAAEPQTADS"
     gene            6200..7480
                     /locus_tag="VF517_11765"
     CDS             6200..7480
                     /locus_tag="VF517_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053658.1"
                     /translation="MTEPQSAGGRPPAVLLGGETIAVSAARSLAPAGVRVYALGDATD
                     PVSRSRHCHEFVEVGAKKGVQERYMAWLRDHAPEGAVVIPCDDDGIELIARGRERLVE
                     LGLRPIEGNDEALLGMLDKDRTYELCREIGVDCPRTETVSTREEAEAVAQRFAYPCAL
                     KPRESHVFAQYFGILAKAVQVGSPEELVREFDRTADLGVSMLVTEIVPGPDEALCSYY
                     SYLDEDGRPLYHFTKKKERQYPPEFGLGCYQITTWDPDVAAAGLKFFQGAGLRGIANV
                     EFKRDSRDGTLRLIECNARLTAANEQVRLAGLDIALLAYSRVTGTPGPELRSYKVGVP
                     LWHPIEDFRAMLRYRARGDLTVGGWLRSLRRRQRFPLFRLDDPLPTVHSLSVKGGRLV
                     RKLRRQAPAASSATNSATSAVEAPPESSPEAVRS"
     gene            complement(7390..8409)
                     /locus_tag="VF517_11770"
     CDS             complement(7390..8409)
                     /locus_tag="VF517_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX8053659.1"
                     /translation="MGYRVTLISGDGVGPEVVGAARAVLDATGVGIEWEERGPEEAGA
                     SVRECGVALKGPTSTPVAGARSFNIALRRELDLHTSIRPVRSLAPAAARGIDLVVARM
                     NGEDLYAGIELEPGSAAAAEVLALAGADLPADTGLSLKPLSETEIRRSTRAALAWCRD
                     AGRTRVTIAHKATVMRQTDGVFLRVAREEAEAAGLEVGDMLVDTICGELARRPGRFDV
                     LLAPVMYGDLLSDLAAGLAGGPGIAPNANLGDTAAVFEPVHGSAPRIAGRGIANPFAT
                     ILSGAMLLRHLGEDAAALNVEGAVAAAAEQGVVTYDLTASGDDSGGASTAEVAEFVAE
                     LAAGA"
     gene            complement(8491..9447)
                     /locus_tag="VF517_11775"
     CDS             complement(8491..9447)
                     /locus_tag="VF517_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053660.1"
                     /translation="MKIFAFDPADYRDQYEKHGWVHIPGGIDPEFLAHLQEYTRSRFS
                     EKHVEGKAIGGAKEQALFDFPAETEFPGELFDSIAHVAGLNREGMTLSERHIKAYDSD
                     APDFPVAHKDRFASQVSVGLSIDIPEDSYLVLYPHTDVSVNPYNVSPQLLASLEPHER
                     PEVVLADAPEIRIDDKPGDVVMFPGSAVWHLRRHAANATNLYLKMNDFNSDPLGEDPS
                     TEERTRRTLELIASANGHTPQLVPVLSRRLDVVARQRSRDGQEVLQAHVWEEMPVVLN
                     QVEWDVLHAIDGQRSVAELAGSDEARAAVQRLAQRGVIELVG"
     gene            complement(9456..10571)
                     /locus_tag="VF517_11780"
     CDS             complement(9456..10571)
                     /locus_tag="VF517_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053661.1"
                     /translation="MGGLGLRLGKRQRALLAQLDVARSTGVRYARLRRAEAAELRAQG
                     GEPRNSTYREIWTEAAAAVGAEYEQLSDDFAELRRDGARTRVMRQSTELDAEVTLEIA
                     SHKGLVHGWLTGAGIPVPEHHAFDYRDLDSGAEFLARAGAACVIKPGSGSGGGWGLAG
                     NICTREQLARAVLNASRYSSQMLIERQVAGDLHRVLVLDGEVIDVVRRRSPSVEGDGR
                     STVRELIAAENRRRLDARGAAGLWPLRIDLDCLFALEARGESLDAVPPSGATVTVKHV
                     TNENRLEDNVTVPASEVGEGIRAEARKAAELVGLRLAGLDVIATGLGRSLADSGGVVL
                     EVNGAPGLSHHYHVADRAKATRVAAPILEKLLSRPKE"
     gene            complement(10574..11704)
                     /locus_tag="VF517_11785"
     CDS             complement(10574..11704)
                     /locus_tag="VF517_11785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8053662.1"
                     /translation="MAEADSQSSARRPAHPIAPRPRPPESVELLDSAERIRADWDDLA
                     DRVEAAPFLRPGWIEPWQTAFGSGEPAYLVCRRDGALAGVLPLQRRRGTLVSTANWHT
                     PLYGPVAADGDAAAELARGLLAHAGSRADLWFLDPEAPGFPQIASAAETGGYGTIART
                     IAQSPYIAVEGSFEDFMAGLDRKFRKDIGRRWRRLEEQGGAEAVFEDGSEGLEDLLTD
                     GFRLEGSGWKSEQGSAIASDPKRESFYREVARWAAARGWLRLAFLRVGGRAIAFDFGI
                     EAHGVFYIPKGGFDVEYRKAGPGQLLTHAGVERAFELGLRSYELLGQQDEYKRQWTAS
                     VRERLRLQVFPRRPVGSANYLAWRYGRPLVRKVRGHGRADPF"
     gene            11729..12847
                     /locus_tag="VF517_11790"
     CDS             11729..12847
                     /locus_tag="VF517_11790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8053663.1"
                     /translation="MARTDIARSGSGSRLRLIDIDPLRDRRWDALVRRHRAAGAYHLG
                     AWAGVLEQAYGDRPCYLGLEAEDGALLAGMPMVCTNGLVTGRRLRSLAVFPPAGPLAD
                     SDEDLRALLQGACRRADATRARVWTLHTRQGGLEAAEPALRFVPKHPTFVLPLDSDPD
                     EMRRGWKKSASNLFRSLKKAEKAGVTIREGSAKSDLDAFYSLYARTMRRRRVLPRPYR
                     QISAARGLLPEGVFRLHLAEHGGRIVAGALWHSFGGTLDLLYNGSDDRHLEGRPNHAL
                     YWHAIRWAAETGHAELDLGHARPGSSLAQWKEQFGAEQVQEFRYDYVPGANPDEVNVE
                     RIASVAEGRESNSLVARGLERAPLPALRLAARVAYRRL"
     gene            complement(12848..13240)
                     /locus_tag="VF517_11795"
     CDS             complement(12848..13240)
                     /locus_tag="VF517_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053664.1"
                     /translation="MSSSDTRALIAQAIEDFSEEVPSLKNLKVVVRLELRAKGHDAPV
                     WRVELPGPAISKDPAGDARVEVSIPRSHFNELAKDGRLKHWVDAYEKGHVNVAGDSAV
                     VKLVGNVIQRQLLRGDSGLRGGGARRNV"
     gene            complement(13252..14673)
                     /locus_tag="VF517_11800"
     CDS             complement(13252..14673)
                     /locus_tag="VF517_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053665.1"
                     /translation="MRERRNPPAEELYARKWRIWTVAMVGIFMALIDVTIVNISIPTL
                     ERELDAKVDAVSWVLNAYNIVFAVVLVSMGRLADQFGRKRFFLIGLAIFTVGSLLCGI
                     AGSVEQLIAFRALQGLGAGILAPLALALTTLIFPPHQRGLGLAGLAIVANLAATIGPP
                     LGGVLLEVASWHWIFLVNVPIGVIGIVAATRVMPETYDLSAGRRVDLVGMLLLGATVL
                     ALTYGLVDANDAGWGSPEIVGLLGAAALLGVAFGLSQRYGRHPMLTAGLVRNRQFVGA
                     SVAFLLFAVGVVGQLFIAILFLTNLWHYSALEAALALTPVALVGLVVAPLVGRIADRV
                     TPRAIGIPALVTMGVGLLWLGAAPTDPSYLAVLGPLILTGGAMGAAFPAMNIGAMGSV
                     RGPELGLGSGIVNMARQLGFALGVAILVAVLESHGSPGSPGEARDAFADSFRVAALFV
                     LAAIPFALAMRRGPSHASPPAVG"
     gene            complement(14670..15032)
                     /locus_tag="VF517_11805"
     CDS             complement(14670..15032)
                     /locus_tag="VF517_11805"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8053666.1"
                     /translation="MSEVLAAGGVVRRDGLVAVVHRPKYDDWTLPKGKLDEGESFEEA
                     ALREVEEETGLRCELGEELESTTYRDRHGREKLVRYWAMTPASGEFEPNREVDELRWV
                     TPDEARALLTYERDRDVL"
     gene            complement(15037..>15698)
                     /locus_tag="VF517_11810"
     CDS             complement(15037..>15698)
                     /locus_tag="VF517_11810"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8053667.1"
                     /translation="PGGMASANKFLEEWVPKITGSPAFKKDGLLIITLDEAEGDDTSS
                     CCGEKTGPNTPNNGGPEPGNGGGRIGAVLLSPFIRAGTETKQEYNHYSLLRSVEDLFA
                     VEHLGYAGQEGLVPFGDDVYTNPAGTPQALARPHLKLSGAPRHCHRGAFTAKLRAIGR
                     GVSTLAASLDGRRLKSSAKAAFGVRVECGALKPGVHRIRGDATGAGGQSHRLFHFRRT
                     R"
BASE COUNT         2246 a         5645 c         5601 g         2206 t
ORIGIN      
        1 gtgccgttcg cgccgtcggc gaacgtcacg cccttcgact cgtactggtt ggtgatctgc
       61 gtcccgacgg ggtactcgtc gaacgcgacc tggttgacgt cggccgaagc cgcgcccgcc
      121 atcgtgaatg tcagcgcgat cgcgagcgcg atcgcgcggc tgcccctgcc gaaacccccc
      181 atgccggcgg ggtttctatc accggtttga ggtaggccgg gctacgcgcc tggttctggg
      241 cgcgtcggcg ttcgtcagcc ccgcggcttc ggtcggtaca gcgcgcagcc cgcgtggagg
      301 gcggtcccgt gaatccggac gacgtgctcg tcgccggggc gcagacgctc gccgcagccg
      361 aggcagcgcg catcgcggtc ggagccgctc aggcggcgga cgacgctgcg gggcgattga
      421 agggcttggc tcaccatcag gggtacggcc gcgggctacc cggcggctca caccgcaaaa
      481 ccgctccggc gcaccgccgg ggcggccggt cgataccctt ccgcgccgcc ccatgcggtt
      541 cttcgagttc gaatcccggc agatcctcgc caaggagggc atcccgctcg ccggccacgg
      601 cttcgcgacc gatgccgagc aggccaagac gatcgcggcg gagatcggaa agccggtcgt
      661 gatcaagtct caggtgctca ccggcgggcg catgaaggcc ggcggcgtgc agttcgccga
      721 cacgcccgag gaggccgagg gccacgcccg cgacatcctc gcgctcgaga tcaacggcca
      781 catgccgaag ggcgtgctga tcgccgagaa ggcgccgatc gcgcaggagt actacgccgg
      841 ggtcatctac gacggggtgc gcaagctgcc cacgttcgtg ttcagcgaca tgggcgggat
      901 cgacatcgag gaggtcgccg agagccatcc cgagcacatc gggcgcggcc acttctccac
      961 gatcctgcca aacatggact tccgcgcgaa ggacgtggtc gcgcaggtcg gcatcaccgg
     1021 ttccgacctc aaccggctca cgcccatcct ctccaagctc gcgcagctct tcgtgaagta
     1081 cgacatgacg ctggccgaga tcaacccgct cgcgaagctc gaggacggca cgttcgtggc
     1141 ggtcgacgcc cacatggaca tggagaacga ggcccgcccg cgccagaagg cgattctcaa
     1201 ggagctcggg atcggcgacg aggagacgcg cgaggcccgc gagccgacgg acttcgagat
     1261 caagggcgag cagatcgatg cggtggacca ccgcggcgtg gccgggaacg tgaccgagtt
     1321 cgacggcaac atcgggcttg tgatcggcgc cggcggcggc tcgctcacgc tgttcgacgc
     1381 ggtccgcaac agcggcggca gccccgcgaa ctactgcgag atcggcggca acccgagcgt
     1441 gtcgaaggcc aagaacctcg cgaagctcgt gctcgagaag ccgggcgtgg agaagatcgc
     1501 cgtgatgatg tcgatcgtct ccaacacccg cgtggacatc gtcgcgcgcg gcgtgatcgc
     1561 ggcctgcctg gagctggggc acgacccggc ggagaagatc gccgtcttcc ggatccccgg
     1621 cgcgtgggag gaagagggct tcaagatcct cgagaagtac ggggtcgagt acttcgaccg
     1681 cacggtctcg atgcacgagg ccgccaggcg ggcggtcgag aagatcgggg ccacggcatg
     1741 aatcacctga tcgaccagga cacgaccttc atcgtgcagg ggatcaccgg ccgcgaggcc
     1801 gtgaacctca cgcgcgagtg cctcgactac gggtcgaaga tcgtcggcgg cgtcacgccg
     1861 gggcgcaagg gccgcgaggt gcacggcgtg cccgtgttcg actcggtcgc gcagctcgtc
     1921 gagtcgatcg gcagcgtgcc ggacggctcg gtcgtcaccg tcccccccgc cttcacgcgc
     1981 gacgcggtgt tcgaggcgat cgaggccggc gtgcggctga tcgtgatcgt caccgagcgc
     2041 atcccgcgct atgacgtcgc gcagatggtc gagctggcca acctgcgcgg cgcgcggatc
     2101 atcggcccca actgcctcgg gatcatccgg ccgggcgtgg cgaagatggg cggcatcggc
     2161 ggccccgcca aggacgcgca gaaggcatac cagcccggct cgatcggcgt gatgtcgcgc
     2221 agcggcggca tgaccaccga gatctcctcg accctgaccg ccgccggcct cggcgtctcc
     2281 accgccgtct cgatcggcgg cgacgcgatc atcggctcca cctacgccga gctgatgccg
     2341 ctgttcgagg ccgacgagca gaccgagggc atcgtgatct acaccgagcc cggcgggcgc
     2401 atggaggccg agctggcggc gtgggtgacc gagaacgagt cgcgcctgcc gatcgtggcc
     2461 ttcatggccg gccgcttcat ggacgagatg ccgggcatga gcttcggcca cgccggcacg
     2521 atcgtagagg gcaaggccga caccgcgacc gagaagatcg cgcggctcga ggccgcggga
     2581 atcaccgtgg ccgaggacat cgcctcgatc cccgacctga tgaaggagaa gctggggaag
     2641 gtggcggcct gatggcggga gtcttcatcg acgtcgaact cgacgacggg atccgcgacg
     2701 acgccgagct ggcgaagaag ctcgaggacg cgtgcccggt ggacatctac gcggcgcgcg
     2761 acgggcgggt cgaggtggtc gaggagaacc tcgacgagtg catcctctgc gagctgtgcg
     2821 tgaagaccgc gcccgccggg aagctgcgcg tccgcaagct ctacgacggc acggcgctcg
     2881 aacgctgagc tcagccggcc gggcgggcgg ccgcgaagag gtggatcccc tcgtagcgcc
     2941 cgaacgcggc cccggcctgg aggcgcagga accgcttggc gtcctcgcgc ggatggacga
     3001 ccgagttcat cgtccgctgc gtgatcatcc gctcgacccg gtagccgttc agctcgaaga
     3061 agcgcgtcag cgcggcgcga tcccagaagt tcaggtgcac cggcggcgtc gaggcctggc
     3121 cgcgcaccgg cgggaacggc accttggccc cctcgttcgg caccgagcag accagcaggc
     3181 ccccgggctt cagcagccgc ttggccttct cgaggaaccc gcgcgggtcg gtgaggtgct
     3241 cgagcacctc gaaggtcgcg atcacgctgt aggcgccctc gtcgaagtcg agctcgtcga
     3301 agcggccctg gcggaccggc aggtggtgga cgtcgcgcga ggcctggagc gccgtctcgg
     3361 agaggtcgat gccctcgacc tgccagccct tccggttcgc ggccgagagg aacaggccga
     3421 caccgcagcc aacctcgagc aggcggtcgt cgggcttcgg cttgtagcgg ttgaggaaga
     3481 tcagcgagcg gtagaggccc ggcttgcgct tgcccttcgc ctcgagctcg cgaatttgat
     3541 cgaggtgcat ctggtaggcg tcgccctcgg agtagatgcg gttgtagatc tcctggaggt
     3601 gctcgtcggt cggcgcctcg accgtgaagc gcacgccgca gcgcccgcag cgcgcgagcg
     3661 cccagccgtt ctcctcaccc gccggcgtcg cgccgtcggc cgcgcacgcc gggcaggcgc
     3721 gagtctcgtt cgagtcccct gtcatcgcca aaacttacct ccatgccctt ctcgtccggt
     3781 ttcgcctgga ccatcggcac atgactgagg aggctcaagg cgcgggcgcc gggcgcaaca
     3841 cgctgctcgc gctgatgacg cagctcgtca cgggtctgtt caccgcggtc ctgacgatct
     3901 acctcgtgcg cgcgctcggg ccgcgcgagt tcggagtcct gtcgctcgcc atcgggatcg
     3961 gcacggtgct gctcctgccg tcggacttcg ggatctcggg ctcggcgtcg cgcttcatcg
     4021 ccgagcgctt cccggaccgc ccggcaatcg ccgcgatcat ggccgacgcg ctgcggctga
     4081 agctcgcgat cggggcgatc gtcagcatcg cgctcctcgc cacggcgggt ctgatcgccg
     4141 acgcgtacgg cgagcccgag ctcgggtggc cgatccgctg gatggccatc gcggtgttcg
     4201 gccagagcat ggtcggcttc ttccgctacg cgttcctctc gatgcgcgac gcggggaccg
     4261 gcttccgcat cgtggccggc gagagcgcgg tcgaggccgc ggccaccatc gggctcgtgg
     4321 tgctggccgg cggcgcggcg tcggcggccg cgggccgggc gatcgggtac acgttcggcg
     4381 cgatcctcgc cggctcgctc gcggcgcggc tgctcggccg cgcggcgttc agccgctcgc
     4441 gccggctggc ggacgcgcgg cgcagactgg cgcgctacgc gggcgcgctg ttcgcgatcg
     4501 acgtggcgtt ctcggccagc gtgcagatgg cgccgctgat gatcggcggc ttcctcggag
     4561 cccgggaggt gggcctgttc acggctccgt cacggctgat gatcctgctc cagtaccccg
     4621 ggctggcgct ggcgaacggc gtcggcccgc ggatggcgcg cggcgaggac cacgagccgg
     4681 agacgcgcct gttcacgacg gcgttccgct acctgatcgt gctccaggcg ctcctgctcg
     4741 tcccgatcgt ggcctgggcg gacccgatcg tcgacctgct gctcggctcc gagttctccg
     4801 agtcggccga cctgctgcgc cagctcacgc cgtacatctt ctcctccgga ctagcggcga
     4861 tcctggccag cggcctcacc tacctcggcg aggcgaagcg gcggctcccg atcgcgatcg
     4921 gcgacgtgtt cctcaccgcg ggcctgacgg ccggggggct cgccacgatc gggctgagcg
     4981 gcgcggccta cgccgccgac atcgtgagcg tcctgtacgt gggggtccac ttctggatca
     5041 tccgcgggct cgtggacctg ccgctgcggc ctctgctgct ggcgctggtg cgcggcctgc
     5101 tggccgccgc ggcggcggcg ggcgtgctgg tgctcttcgg gaccgacgac ctggcggttt
     5161 gggagtgggt cgcgggcggc gcgggcgccc tgctcgcctt cacggcggtc gtggtgctca
     5221 ctcgcgaggt ctcgctcggc gagctgcgct ccatggtggc gctggtcgcg cggcgcagcc
     5281 gcgcctgaac gcgcccttgc acccgatctc atcgattgga gccttttgca ccgtatgtcg
     5341 gagtaaggct ccaatcgatc gctcctaaag ggcggccgcc gcggggtcga ttaccgtttg
     5401 gaacggcccc gcaccgagag gaagaccatg gccggcaagc ccaagtccgc tgagatgcac
     5461 ccgggtcccg ggtttcgcat ccgggagaag atcaaccgcc cgcgccccga gacgatcgcc
     5521 gcgctcggcg cgttcgacgc gcccgcgatc tccgacctca tgaaccggct ctacgcgatg
     5581 acgccggcca tcaagaacct gaccgatccc aacctgaggc tgctcgggcc ggccaccacg
     5641 gtcaagtgct acccgggcga caacctgatg gtccacaagg cgctcgacat cgccgagccg
     5701 ggcgacgtga tcgtcgtgga cacgagcggt cagcagaaca ccgccgtgct cggcgacctc
     5761 gtctcgacca aggcgcgcca ccgcggagtg gccggcttcc tggtcgacgg cctggtccgc
     5821 gacatcgcgg ggatccgcat gctgggcgac ttcccggtct acgcccgtga cgtcaccccg
     5881 atcggcccgc tgcaccgcgg cccgggtgag atcaacttcc cgatctcggc cggcggcatc
     5941 gtggtccagc ccggtgacgc gatcgtgggc gactccaacg gcgtcgtggt cgtgccgcag
     6001 gacatcgtcg aggagatggt gcgccggctc acggcccagc aggccgccga gtcggactac
     6061 accgccgccg tcgcccgtgg cgagttcaac aacagctggg tcgacaagac cctgaccgac
     6121 gccggcgtgc cgctggtgaa cggcaacggc aaccacaagg cccccgccgc cgagccgcag
     6181 accgcggaca gctagcgggt tgacagagcc tcagtccgcc gggggcaggc ccccagccgt
     6241 cctgctgggc ggcgagacga tcgcggtctc ggccgctcga agcctggcgc ccgccggggt
     6301 gcgggtgtac gccctcgggg acgcgacgga cccggtgagc cgctcgcggc actgccacga
     6361 gttcgtggag gtcggggcga agaagggcgt gcaggagcgc tacatggcgt ggctgcgcga
     6421 ccacgcgccg gagggcgccg tcgtgatccc gtgcgacgac gacggcatcg agctgatcgc
     6481 ccgcggccgc gagcggctcg tggagctcgg gctgcggccg atcgagggca acgacgaggc
     6541 cctgctcggc atgctcgaca aggaccgcac ctacgagctg tgccgcgaga tcggcgttga
     6601 ctgcccgcgc accgagacgg tctccacccg cgaggaggcc gaggcggtgg cccagcgctt
     6661 cgcctacccg tgcgccctca agccgcgcga gtcgcacgtg ttcgcgcagt acttcgggat
     6721 cctggcgaag gccgttcagg tcggctcgcc ggaggagctc gtcagggagt tcgaccggac
     6781 cgccgacctc ggcgtctcga tgctcgtcac cgagatcgtg ccggggcccg acgaggcgct
     6841 ctgctcgtac tactcctacc tcgacgagga cggccggccg ctctaccact tcaccaagaa
     6901 gaaggagcgc cagtacccgc ccgagttcgg gctcggctgc taccagatca cgacctggga
     6961 cccggacgtg gccgccgccg ggctgaagtt cttccagggc gccgggctgc gcgggatcgc
     7021 gaacgtcgag ttcaagcgcg actcgcgcga cggcaccctg cgcctgatcg agtgcaacgc
     7081 gcggctcacc gccgccaacg agcaggtccg gctggcgggg ctcgacatcg cgctgctcgc
     7141 ctactcgcgc gtgactggca cgcccgggcc ggagctgcgc tcgtacaagg tcggcgtgcc
     7201 gctctggcac ccgatcgagg acttccgggc gatgctgcgc taccgcgccc gcggcgacct
     7261 caccgtcggc ggctggctgc gcagcctgcg caggcgccag cgcttcccgc tcttccgcct
     7321 cgacgacccg ctgccgaccg tgcattcgct gtcggtcaag ggtgggcggc tggtgcgcaa
     7381 gctcaggcgt caggcgccgg cggccagctc ggccacgaac tcggccacct ccgccgtcga
     7441 ggctccgccg gagtcgtcgc ccgaagccgt gaggtcgtag gtcacgaccc cctgctcggc
     7501 ggccgcggcc accgcgccct cgacgttcag cgcagccgcg tcctccccca ggtgacgcag
     7561 cagcatcgcg ccggagagga tcgtggcgaa cgggttggcg atgccgcggc ccgcgatgcg
     7621 cggcgccgag ccgtgtaccg gctcgaacac cgctgcggtg tcgcccaggt tcgcgttcgg
     7681 cgcgatgccg ggaccgccgg cgaggccggc cgccaggtcc gagagcagat cgccgtacat
     7741 cacgggcgcg agcaagacat cgaagcgccc ggggcgccgc gccagctcgc cgcagatcgt
     7801 gtccaccagc atgtccccca cctcgagccc ggccgcctcg gcctcctcgc gcgcgacgcg
     7861 caggaagacg ccgtcggtct gccgcatcac ggtggccttg tgggcgattg tcacccgcgt
     7921 gcggcccgcg tcccggcacc acgccagcgc cgccctcgtc gatcgccgta tctccgtctc
     7981 ggacagcggc ttcaacgaga gaccggtatc ggccgggagg tcggccccgg cgagcgcgag
     8041 cacctcggcc gccgcggcgg agccgggctc gagctcgatc cccgcgtaga ggtcctcccc
     8101 gttcatgcgc gcgaccacca ggtcgatgcc gcgcgccgcg gcgggcgcga ggctccggac
     8161 cggccggatg ctcgtgtgca ggtcgagctc gcgccgcagc gcgatgttga acgaccgagc
     8221 acccgcgacg ggcgtggacg tcggcccctt cagggccacc ccgcactcgc ggaccgaggc
     8281 gccagcctcc tccggcccac gctcctccca ctcgatcccg accccggtgg cgtcgagcac
     8341 cgcccgcgca gcgcccacga cctcgggacc gaccccgtcc ccggagatca gcgtgacgcg
     8401 ataacccatg ggccggagcg tattcagcga actggtcgac ctaatgcacg tataccgtgc
     8461 attcgatcga ccagatgccg atttcggtgt ctagccgacc agctcgatca cgccgcgctg
     8521 ggcgaggcgc tgcaccgcgg cgcgggcctc gtcggagccg gcgagctcgg cgacgctgcg
     8581 ctggccgtcg atcgcgtgaa gcacgtccca ctcgacctgg ttgaggacga ccggcatctc
     8641 ctcccagacg tgcgcctgga ggacctcctg gccgtcgcgg ctgcgctggc gcgcgaccac
     8701 gtcgagccgg cgcgagagca ccggcacgag ctgcggcgtg tggccgttgg ccgacgcgat
     8761 cagctccagc gtgcggcgcg tgcgctcctc ggtcgaggga tcctcgccga gcgggtcgga
     8821 gttgaagtcg ttcatcttca ggtagaggtt ggtcgcgttc gcggcgtgcc ggcggaggtg
     8881 ccacacggcg gagcccggga acatcacgac gtcgccgggc ttgtcgtcga tccggatctc
     8941 cggagcgtcg gcgaggacga cctcggggcg ctcgtgcggc tcgaggctcg cgaggagctg
     9001 cgggctcacg ttgtacgggt tgaccgacac gtccgtgtgc gggtagagca cgaggtacga
     9061 gtcctccggg atgtcgatcg agaggccgac cgagacctgg gacgcgaagc ggtccttgtg
     9121 ggcgaccggg aagtccggtg cgtccgagtc gtaggccttg atgtggcgct cggacagcgt
     9181 catgccctcg cggttgaggc ccgcgacgtg ggcgatcgag tcgaacagct cgcccgggaa
     9241 ctccgtctcg gcggggaagt cgaacagcgc ctgctccttc gcgccgccga tggccttgcc
     9301 ctcgacgtgc ttctcgctga agcggctgcg ggtgtactcc tggaggtggg cgaggaactc
     9361 ggggtcgatg ccgccgggga tgtggaccca gccgtgcttc tcgtactggt cgcggtagtc
     9421 cgcggggtca aaggcgaaga tcttcatgaa agggtctatt ccttgggtcg ggacagcagc
     9481 ttctccaata tcggcgcggc gacgcgggtg gctttagccc tgtccgcgac gtggtagtgg
     9541 tgactgaggc ccggggcccc gttcacctcc agcaccacgc cgcccgaatc ggcgagcgac
     9601 cggccgagac cggtagctat gacgtcaagc ccggccaagc gtagccccac cagctcggcc
     9661 gccttgcgcg cttctgcacg aattccttcg ccgacctccg aggcgggcac ggtcacgttg
     9721 tcctcgagcc ggttctcgtt cgtcacgtgc ttcacggtga cggtcgcgcc ggacggcggg
     9781 acggcgtcga ggctctcgcc gcgggcctcg agggcgaaca ggcagtcgag gtcgatgcgc
     9841 agcggccaga gcccggcggc gccccgggcg tcgagccggc ggcggttctc ggcggcgatc
     9901 agctcgcgga cggtcgagcg cccgtcgccc tcgacgctcg gcgagcggcg ccggaccacg
     9961 tcgatcacct cgccgtcgag cacgagcacg cggtgcaggt caccggccac ctggcgctcg
    10021 atcagcatct gcgagctgta gcgcgaggcg ttcagcaccg cgcgggcgag ctgctcgcgc
    10081 gtgcagatgt tgcccgcgag tccccagccg ccgcccgatc cgctccccgg cttgatcacg
    10141 caggccgccc ccgcgcgcgc gaggaactct gcgccggagt cgaggtcgcg gtagtcgaag
    10201 gcgtggtgct cgggcaccgg gatcccggca ccggtcagcc agccgtgcac gaggcccttg
    10261 tgagaggcga tctcgagcgt gacctcggcg tcgagctcgg tggactgccg catcacgcgc
    10321 gtgcgcgcgc cgtcgcggcg cagctcggcg aagtcgtcgg agagctgctc gtactcggct
    10381 cccaccgccg cggcggcctc ggtccagatc tcccggtagg tcgagttgcg cggctcgccg
    10441 ccctgcgcgc gcagctcggc cgcctcggcg cggcgcaggc gcgcgtagcg caccccggtc
    10501 gagcgcgcga catccagctg agcgaggagc gcgcgctggc gcttcccgag gcgcagtccc
    10561 aggccgccca cggctagaag gggtccgccc gtccgtggcc gcgtaccttg cgcacgagcg
    10621 gccgcccgta gcgccaggcg aggtagttcg ccgagccgac cgggcggcgc gggaagacct
    10681 ggaggcgcag gcgctcgcgc acggaggccg tccactggcg cttgtactcg tcctgctggc
    10741 cgagcagctc gtacgagcgc aggccgagct cgaaggcgcg ctcgacgccc gcgtgcgtga
    10801 ggagctgccc cgggccggcc ttgcggtact cgacgtcgaa accgcccttg gggatgtaga
    10861 agacgccgtg cgcctcgatg ccgaagtcga aggcgatcgc gcggccgccc acgcgcagga
    10921 acgccagccg cagccagccg cgcgccgccg cccagcgcgc gacctcgcgg tagaagctct
    10981 cgcgcttcgg atcggacgcg atcgcgctgc cctgctcgga cttccagccc gagccctcga
    11041 gccggaagcc gtcggtcagg aggtcctcga ggccttccga tccgtcctcg aagaccgcct
    11101 cggccccgcc ctgctcctca agccgcctcc atcgccgacc gatgtcctta cggaacttgc
    11161 gatcaagccc cgccatgaag tcctcgaacg agccctccac cgcgatgtac ggcgactgcg
    11221 cgatcgtgcg cgcgatcgtg ccgtacccgc ccgtctcggc ggcgctcgcg atctgcggga
    11281 agccgggtgc ctcgggatcg aggaaccaca ggtcggcgcg gctgcccgcg tgggcgagca
    11341 gcccgcgggc cagctccgcg gcggcgtcgc cgtcggccgc gaccggcccg tacagcggcg
    11401 tgtgccagtt cgccgtcgag acgagcgtgc cgcgccggcg ctgcagcggg agcacgcccg
    11461 cgagcgcccc gtcgcgcctg cacaccaggt acgcgggctc gcccgagccg aacgccgtct
    11521 gccacggctc tatccatccg ggccgcagga atggcgcggc ctcgacgcgg tcggcgaggt
    11581 cgtcccaatc ggcgcggatc cgctcggccg agtccagcag ctcgaccgac tcaggcgggc
    11641 gtgggcgcgg agcgatggga tgcgccggac gccgcgcgct cgattgcgag tcggcttcgg
    11701 ccacggggtc gcgaggttag cggtgaccgt ggcgagaacg gatatcgcgc ggagcggctc
    11761 gggctcccgg ctgaggttga tcgacatcga tccgctgcgg gaccggcgct gggacgcact
    11821 cgtccgccgc caccgcgcgg ccggcgccta ccacctgggg gcgtgggcgg gcgtgctcga
    11881 gcaggcctac ggcgatcgcc cctgctacct gggtctcgag gccgaggacg gcgcgctgct
    11941 cgccgggatg ccgatggtgt gcacgaacgg cctcgtgacc ggccgccggc tgcgctcgct
    12001 ggcggtgttc ccgccggcgg gaccgctggc cgactccgac gaggacctgc gcgcgctgct
    12061 ccagggcgcg tgccgccgcg ccgacgccac gcgcgcccgc gtctggaccc tgcatacgcg
    12121 ccaaggcggc ctcgaggcgg cggagccggc gctgcggttc gtgcccaagc acccgacctt
    12181 cgtgctgccg ctcgacagcg atcccgacga gatgcgccgc ggctggaaga agagcgcgag
    12241 caacctcttc cgcagcctca agaaggccga gaaggccggc gtcacgatcc gcgaggggtc
    12301 cgcgaagtcc gacctcgacg cgttctactc gctctacgcg cgaacgatgc gccggcgccg
    12361 tgtgctcccg cgtccgtacc gccagatctc cgcggcgcgc ggcctgcttc ccgagggtgt
    12421 cttccgcctg cacctggccg agcacggcgg ccggatcgtc gccggcgcgc tgtggcactc
    12481 gttcggcggc acgctcgacc tcctctacaa cggcagcgac gatcgccacc tcgaggggcg
    12541 cccgaaccac gccctctact ggcacgcgat ccgctgggct gccgagaccg ggcacgccga
    12601 gctggacctc ggtcacgcgc ggcccggctc gagcctcgcg cagtggaagg agcagttcgg
    12661 cgccgagcag gttcaggagt tccgctacga ctacgtaccc ggggcgaacc ccgacgaggt
    12721 gaacgtggag cgcatcgcga gcgtcgccga gggtcgcgag agcaacagcc tcgtcgcccg
    12781 tggcctcgag cgcgctccgc tgccggccct gcgcctggcc gcgcgcgtgg cctaccggcg
    12841 cctctagcta cacgttgcgg cgggcgccgc cgccgcgcag accgctgtcg ccgcgcagga
    12901 gctggcgctg gatcacgttg ccgacgagct tcacgacggc gctgtcgccc gccacgttca
    12961 cgtggccctt ctcgtaggcg tcgacccagt gcttgaggcg cccgtccttg gccagctcgt
    13021 tgaagtgcga gcgggggatc gagacctcca cgcgagcgtc gcccgccggg tccttcgaga
    13081 tcgccgggcc gggcagctcg acccgccaca ccggcgcgtc gtgacccttg gcgcgcagct
    13141 cgagccggac gaccaccttc aggttcttga gcgacggaac ctcctcggag aagtcctcga
    13201 tggcctgggc gatcagcgcg cgggtgtcgg aggagctcat cgcgccggag cctaacccac
    13261 ggccggaggc gacgcgtgcg acggcccgcg gcgcatcgcg agcgcgaacg ggatcgccgc
    13321 caggacgaac agggcggcca cgcggaagga gtcggcgaag gcgtcgcgcg cctcgccggg
    13381 cgaaccgggc gatccgtgtg actcgagcac ggccacgagg atcgccacgc ccagggcgaa
    13441 gccgagctgg cgcgccatgt tcacgatccc ggagccgagc cccagctccg gcccgcgcac
    13501 cgatcccatg gcgccgatgt tcatggccgg gaacgccgcg cccatcgcgc ccccggtgag
    13561 gatcaggggt cccagcacgg ccaggtagct cgggtcggtc ggggcggccc ccagccacag
    13621 caggccgacc cccatggtga cgagcgccgg gatgccgatc gcgcgcggcg tcacgcggtc
    13681 ggcgatccgg ccgaccagcg gcgccacgac gaggcccacc agcgcgaccg gagtcagggc
    13741 cagcgcggcc tcgagcgccg agtagtgcca caggttcgtg aggaacagga tcgcgatgaa
    13801 gagctgcccg accacgccga ccgcgaacag gaggaacgcg acgctcgctc ccacgaactg
    13861 ccggttgcgg acgaggcccg cggtcagcat cgggtggcgg ccgtagcgct gcgacaggcc
    13921 gaaggcaacc cccagcagcg cggcggcgcc cagcagcccg acgatctccg gggagcccca
    13981 gcccgcgtcg ttggcgtcga cgagcccgta cgtgagcgca agcacggtgg ccccgagcag
    14041 cagcatcccg accaggtcca cgcggcgccc cgcgctcagg tcataggtct cgggcatcac
    14101 ccgtgtggcc gcgacgatgc ctatgacgcc gatcgggacg ttcaccagga agatccagtg
    14161 ccagctcgcc acctcgagca gcaccccgcc cagcgggggc ccgatcgtgg ccgccaggtt
    14221 cgcgacgatc gcgaggcccg cgaggccgag cccgcgctgg tgcggcggga agatcagcgt
    14281 ggtgagcgcg agcgccagcg gcgcgaggat gccggcgccg agcccctgga gcgcgcggaa
    14341 ggcgatcagc tgctcgaccg acccggcgat cccgcagagc agcgagccga ccgtgaagat
    14401 cgccagcccg atcaggaaga agcgcttgcg gccgaactgg tcggcgagcc gccccatcga
    14461 gacgagcacg accgcgaaga cgatgttgta cgcgttcagc acccatgaga cggcgtcgac
    14521 cttggcgtcc agctctcgct ccagggtggg gatcgagatg ttcacgatcg tgacgtcgat
    14581 cagggccatg aagatcccga ccatcgcgac ggtccagatc ctccacttgc gggcgtagag
    14641 ctcctcggcg ggcgggttgc ggcgctccct cacagcacgt cccggtcacg ctcgtacgtc
    14701 aggagggcgc gcgcctcgtc cggcgtcacc cagcgcagct cgtccacctc gcggttcggc
    14761 tcgaactcgc cggaagccgg ggtcatcgcc cagtagcgga cgagcttctc gcgcccgtgg
    14821 cggtcgcggt aggtcgtcga ctcgagctcc tcgccgagct cgcagcgcag gccggtctcc
    14881 tcctccacct cgcgcagcgc cgcctcctcg aacgactcgc cctcgtcgag cttccccttg
    14941 gggagggtcc agtcgtcgta cttcggccgg tgcacgactg cgacgagccc gtcgcggcgc
    15001 acgactccac ccgccgcgag aacctcgctc acgcggctag cgcgtccggc ggaagtggaa
    15061 cagccggtgc gactgaccgc ccgcgcccgt agcgtcgccg cggatccggt ggacgccggg
    15121 cttcagcgca ccgcactcca cccttacccc gaacgccgcc ttcgccgagc tcttcaggcg
    15181 cctgccgtcg agcgaggccg cgagcgtgct caccccgcgc ccgatcgcgc ggagcttggc
    15241 cgtgaaggcg ccgcggtggc agtgcctggg cgcgccggag agcttcaggt gcgggcgtgc
    15301 gagtgcctgc ggcgtacccg ccgggttcgt gtagacgtcg tcgccgaagg gcaccaggcc
    15361 ctcctgcccg gcgtagccga ggtgctcgac cgcgaacagg tcctcgacgc tccgcagcag
    15421 cgagtagtgg ttgtactcct gcttggtctc ggtgccggcc cggatgaacg gcgagagcaa
    15481 tacggccccg atccgcccac cgccgttgcc cggctccggg ccgccgttgt tcggcgtgtt
    15541 cgggccggtc ttctcgccgc agcaggagct cgtgtcgtcg ccctcggcct cgtcgagcgt
    15601 gatgatcagc aggccgtcct tcttgaaagc gggcgagccg gtgatcttcg ggacccactc
    15661 ctcgaggaac ttgttcgcgc tcgccatccc accgggct
//
LOCUS       DASVPL010000220         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_262577, whole genome shotgun sequence.
ACCESSION   DASVPL010000220 DASVPL010000000
VERSION     DASVPL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262577"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..884)
                     /locus_tag="VF517_11815"
     CDS             complement(132..884)
                     /locus_tag="VF517_11815"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX8053668.1"
                     /translation="MELEDRLTITTPEGIELDLQLAGLGSRFIAQTLDLLIKGVTILL
                     IALALSIAGLTGLAIMLPAFMLVLYAYDVVFETFANGRTPGKMAARLRVVRAGGEPID
                     FMSSAIRNVLRVIDGIPFSYVPGMISILVTQRNQRLGDLAAGTIVIHEERVPVPPPRP
                     GPGWAPPGPGWAPPPPWQPAPVWDVSAVSAEELSAVRSFLDRRWTIDVRARGQLALQL
                     AEVLWPKVAGAPAGLPPEAFLEQLAQQKALRG"
     gene            891..1901
                     /locus_tag="VF517_11820"
     CDS             891..1901
                     /locus_tag="VF517_11820"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX8053669.1"
                     /translation="MNLDRFQSTRGARWAELEELVRRAGGKPERLGPAGVRRVGRLYR
                     STAADLAVARRRFPGDPLIARLERLTVAARQAVYAERSRRLSPVRFFTTDYWRRVHER
                     PRLLLIAALLLIVPGLLGGLWAAEDPGAAIGLVPQDFLPTVESPGGNRGLSGEEAGAF
                     SSAVFTNNIQVSFLALAAGIALGLGTVFVLLYNGLILGVVFGLTIENGQGAPLVEQVA
                     SHGILELSCTVVTAAAGLRLGEAILRPGRRKRTDALVDEARGAVEIVLGTAPWLVLAG
                     FVEGFVSPEGIGVTAALIVGAAIAGVYWMLVAVRGRPEPAAGEADPSAPSHSRARALA
                     LR"
BASE COUNT          228 a          706 c          712 g          269 t
ORIGIN      
        1 ctcccccgcg acggcctcgt cgcgctcatg tgcgtcgagc gcgatcccga ggcctgccac
       61 cgctcgctgg tcgccggccg gctggccgcc gagcacgggc tcgcggtcac gcacctgcgc
      121 ccgccggcgt cctagccgcg cagcgccttc tgctgggcga gctgctcgag gaacgcctcg
      181 ggcggcaggc ccgcgggcgc gccggccacc ttcggccaga gcacctcggc gagctggagg
      241 gcgagctgcc cgcgcgcgcg tacgtcgatt gtccagcggc gatcgaggaa gctgcgcacg
      301 gcggacagct cctcggcgct cacggcgctc acgtcccaga ccggcgcggg ctgccacggg
      361 ggcggcggcg cccagcccgg tccgggcggc gcccagcccg gtcccggcct cggcggtggc
      421 acgggaaccc gctcctcgtg gatcacgatc gtgccggcgg ccaggtcccc caggcgctgg
      481 ttgcgctggg tcacgaggat gctgatcatc ccggggacgt agctgaacgg gatcccgtcg
      541 atcacgcgca gcacgttgcg gatcgcgctc gacatgaagt cgatcggctc cccgcccgcc
      601 cgcaccacgc gcagccgcgc cgccatcttg ccgggcgtgc gaccgttcgc gaatgtctcg
      661 aacaccacgt cgtacgcgta gaggacgagc atgaaggcgg gcagcatgat cgccaggccg
      721 gtgaggccgg cgatcgagag cgccagcgcg atgaggagga ttgtcacgcc cttgatgagc
      781 aggtcgagcg tctgcgctat gaaacgggac ccgagaccgg cgagctggag gtccagctcg
      841 atgccctccg gggtagtgat ggtgagacgg tcctccagct ccatggccac ttgaacctag
      901 accgcttcca gtccacgcgc ggcgcccgct gggccgagct cgaggagctc gtgcgcaggg
      961 ccggcggcaa gcccgagcgc ctcgggccgg ccggcgtgcg gcgcgtcggg cgcctctacc
     1021 gctccacggc ggccgacctc gcggtcgctc gccggcgctt ccccggcgat ccgctgatcg
     1081 cgcgcctcga acggctcacg gtggcggcgc gtcaggcggt ctacgccgag cgctcgcgcc
     1141 gcctctcccc cgtccgcttc ttcaccaccg actactggcg ccgcgtgcac gagcgcccgc
     1201 ggctgctgct catcgcggcg ctgcttctga tcgtgcccgg cctgctcggt gggctgtggg
     1261 ccgcggagga tcccggcgcc gcgatcgggc tcgtgccgca ggacttcctc cccacggtcg
     1321 agtcgccggg cggcaaccgc ggcctgtcag gcgaggaggc cggcgcgttc tcgagcgcgg
     1381 tgttcacgaa caacatccag gtctcgttcc tggcgctggc ggcggggatc gcgctcgggc
     1441 tcggcacggt gttcgtgctc ctctacaacg ggctgatcct cggcgtggtg ttcgggctga
     1501 cgatcgagaa cggccagggc gcgccgctcg tggagcaggt ggcgagccac gggatcctcg
     1561 agctgtcgtg caccgtcgtc acggcggccg ccggcctgcg actcggcgag gcgatcctgc
     1621 gcccgggccg gcgcaaacgc acggacgcac tggtggacga ggcccggggc gccgtcgaga
     1681 tcgtgctcgg caccgcgccg tggctggtcc tggccgggtt cgtggagggg ttcgtctcgc
     1741 ccgaggggat cggcgtcacg gcggcgctga tcgtcggcgc cgccatcgcc ggggtgtact
     1801 ggatgctcgt ggcggtgcgc ggtcggcccg agccggcggc cggcgaggcc gacccgagcg
     1861 ccccgtctca cagccgcgct cgcgccttgg ccctcaggta ggcgcgcacg cacgc
//
LOCUS       DASVPL010000221         8908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_300607, whole genome shotgun sequence.
ACCESSION   DASVPL010000221 DASVPL010000000
VERSION     DASVPL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300607"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..423)
                     /locus_tag="VF517_11825"
     CDS             complement(175..423)
                     /locus_tag="VF517_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053670.1"
                     /translation="MYRAFGMHRAVSHLHVALVRAGDRLRSSAGQGTVEYVALILLVA
                     LVMAGVVAALKGYKTDQGKDLGDAIVGKIKDAVDKVKF"
     gene            638..1594
                     /locus_tag="VF517_11830"
     CDS             638..1594
                     /locus_tag="VF517_11830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8053671.1"
                     /translation="MRVLLVTSMYPTPERPGLGGFVRDQVEALRAFDDLDLELFVIEP
                     TGGSRRWLSAARELRRRYARERFDLVHAHHGLAGWSALAVRGAPHLVTFHGTDLSHRV
                     VGPMSRALARLIDAAAPVSTTLTRFAGGGLPGAGRSLRAPVLPMGVNLDRFGRRDRAE
                     ARARLGLDPSRPYLLFPADPARPEKRHDRARELAAAAGAELIGYEGVAPKDVPDLINA
                     VNTVVAPSEREGYGLAPLEALACDVPVLSTDVGIAPLALRGVEGTLCAPFDRDRWLRA
                     VQPHVADPDPRIDGRRRAELFDRNRMAERVFEAYRELTSMRP"
     gene            1726..2310
                     /locus_tag="VF517_11835"
     CDS             1726..2310
                     /locus_tag="VF517_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053672.1"
                     /translation="MRKRLRRARHARDAALHELGALVMEMQRQGRHDPSLVERKAREA
                     VAVDAEARALAGALDRGEALTSVSATGIVGPCGSCGALVAREDRFCGRCGTAVGAAGV
                     NGATAVVGAAAPSGAQRVGAPTVAVAQPVDAAPPSAAQRVDAAPPATAERVDAAPPPS
                     GAQRVEAAPPAAADRVETGPPSDDGVPTYTVTTR"
     gene            2310..3566
                     /locus_tag="VF517_11840"
     CDS             2310..3566
                     /locus_tag="VF517_11840"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8053673.1"
                     /translation="MRFRRDKDGGGSAGPPDAGTRVDQPTETITAPPPSVADRVDEPA
                     AQPPGADGAEEPTAVQPTRESEGPAKVLPPTEGEASARPPSPPPPSATDRVEAPAPAA
                     PSATDRVESPAPPAAPSATDRVESPAPAAPSATDRVESPAPPAAQATRESEGPAKVLA
                     PPPTASTRVDAPAAAPPPQPGQLVSATAPTQAGQTACPQCGTPADAGQEMCIVCGSRI
                     ARKQSKPRGWLLPAVLAGLGVLLLGTAVAFGVVELTKDDEVKKSDVADLNEDTQPSTP
                     PPSTRPPPAATPPTQTSPTIPPATREPDNPSGTPDTTQTTPAPTTPGGSGNEIAEWPA
                     GKTAYTVVLVSATSRNAADAKAEQAIARGIKAGVLRSDDFATLRAGYWVVFAGQYDSS
                     EQATRAADRYAEQGFGGGYPRQVKPK"
     gene            complement(3626..4720)
                     /locus_tag="VF517_11845"
     CDS             complement(3626..4720)
                     /locus_tag="VF517_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX8053674.1"
                     /translation="MPPRPPPPRPLCKTLSARCSSGDYGTGIAAPLYFRNESSLEHDT
                     GAHPEGPGRIPAIERELEARDWLGYSVRDAPAVSLDVLGAVHPVRYVESVRAMSEGGG
                     GAFDPDTVASPGSYRAALHGAGGACALVEALVAGEARFGFCGLRPPGHHAESARAMGF
                     CLFNNVAVAARHALDSLGVRRVFVFDWDVHHGNGTNDIFHSSADVLFASIHQSPLYPG
                     TGPLSDVGSGAGEGFSLNLPVPPGSGENEWLALVEHVVVPAARAFEPELVLVSAGFDA
                     HRDDPLADCRLSTDSFAAMALWMRSLGESCGAPVGAVLEGGYDLSALATSVAATLEAF
                     SLGGEPQRVAANGISEEAAAVVGRYWPLSA"
     gene            4660..6132
                     /locus_tag="VF517_11850"
     CDS             4660..6132
                     /locus_tag="VF517_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8053675.1"
                     /translation="MSTEPTASYRADVAVVGAGAAGLYAALVAAREGARVVLVSRSPL
                     AESASYWAQGGIAAALAVYDSPEGHLADTLAAGRGSCRRSAAQVLCDESPAAVRDLER
                     LGVHFDADRRGSLALGLEGGHSVRRVVHAGGSATGRRITRELSALAAQDERIEILEGT
                     TASALWCADGRCAGLLAQRTSGARIALSARATILATGGAAALWERTTNPRGAIGAGLT
                     LAEHGGAKLADLEMTQFHPTALVHDDPQHDGFLITEAIRGEGALLLDARGERFVDELA
                     PRDQVALAVQGVLAQGEQVHLDMRAIDMRGFPNVASALRRAGFDPTRQLVPVAPAAHY
                     TMGGIAVDLDGRSSIEGLLAVGECACSGLHGANRLASNSLAECFVFGRRAGLAAAASD
                     PLPGDVGEPPASGPDPVPPRPTRAALWRGAGLHRTAEGLRSLLDDPFPLARRIAAAAL
                     AREESRGAHQRQDFRVADPRLDGMHVTLSGDVAPQLERWL"
     gene            6282..6722
                     /locus_tag="VF517_11855"
     CDS             6282..6722
                     /locus_tag="VF517_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053676.1"
                     /translation="MYQFSRAVYRELSPLVEIDPHGCDREHRRAVLTACEQAFTRLAT
                     DRHYFARPTRSLFNDVRCYFPMARQMDVYRVIDRYMHIAGDYVVQREREGLSIDGSPL
                     CCHASTRKGTACQRVPLPGSKYCPSHKHLEEGFDPLEEELVTAA"
     gene            6733..7365
                     /locus_tag="VF517_11860"
     CDS             6733..7365
                     /locus_tag="VF517_11860"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX8053677.1"
                     /translation="MQIAIPLFDGLTALDAVGPYEVLSRPPGWDVVFVAAETGPKRTD
                     NAVLALTADASLADLPRPDIVVVPGGIGTRSSANDDVVEWIRSVHEHTRWTTSVCTGS
                     LLLGKAGVLEGLRATTHWLSLEMLREYGAEPTSERVVEQGKVITAAGVSSGIDMALRL
                     LQVELGDEIAQAVQLSIEYDPQPPFDAGSPEKAPPAIVDFVRQASAHLKQ"
     gene            7365..>8908
                     /locus_tag="VF517_11865"
     CDS             7365..>8908
                     /locus_tag="VF517_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX8053678.1"
                     /translation="MLLGVDVGGTFTDAALLDGERLVTAKAPTTPRDQSEGVIAAVEA
                     ALERAGAEASAVERFVHGMTVGTNALLEGKVARTALLATEGFTDLEALGRQARADLYR
                     LCAGHPPPLVPDELRVAVPERTGPDGVLRELDEELLRERLDGVDAEAAAVCLLWGFRH
                     PEHERRVAELVADGVHVSTSHETAGVFREYERCATTVVDAALSPLLRRYLERLSDRAR
                     DAGLPAPEVMLSGGGVVAAATAARHGSWTVLSGPAGGAVGCARLADRAGRPDAVGLDM
                     GGTSADVSVASGGRAASTGGREVGGRALALPMVDVHTVGAGGGSVAWRDAGGALRVGP
                     RSAGADPGPAAYGRGGTEPTVTDANLVLGYLDAGSPLAGGVSLDAGAAARVVGELGDS
                     LGLSLEETAAGIVRVAGVEMARAVRVMTVERGIDPRELALLAFGGAGPLHAAAVAEEL
                     GMRRVLVPRSSGVLSAVGLIASERRRDLVESVLLAGEALARETVAGAVERLAARGREE
                     LGEPDA"
BASE COUNT         1150 a         3405 c         3285 g         1068 t
ORIGIN      
        1 cgcggcgtga cgggctcgcc gggtcggagc tccgccgcgc gggcctcgcc gggtcggagc
       61 tccgccgcgc gggcttcgcc gagtcggagc tccgtcgcgc gggcttcgcc gagtcggagc
      121 tccgtcgcgg cggccgcggc ggacgcggcc gctgacccgg agcgcgcgag gcgctcaaaa
      181 cttcaccttg tccacggcgt ccttgatctt gccgacgatc gcgtcgccca ggtccttgcc
      241 ctggtcggtc ttgtagccct tgagcgcggc gacgacgccg gccatcacga gcgcgaccag
      301 caggatcagc gcgacgtact cgaccgtgcc ctgcccggcg ctcgaccgca ggcggtcgcc
      361 ggcgcggacg agcgcgacgt gaaggtggct gactgcgcga tgcatgccga aggcgcggta
      421 catggggttc tcctcccgtg acggtgctcc gcgggcaccg atgtgggcgc ctggggagcg
      481 tgtttttgcg atggggcgat ggtcgcgagg ggcgtgttac gggacaagac cgcggggtga
      541 cgattgagtg acgggggtgt gacaaagcgg atgcactggc atccgggcgc cgcgacccgc
      601 cccctttccc gcgaccgcgc gagtacgctg cgcgcagatg cgggtgctgc tcgtgaccag
      661 catgtacccg actcccgagc ggccggggct cggcggcttc gtccgcgacc aggtcgaggc
      721 gctgcgcgcg ttcgacgacc tcgacctcga gctgttcgtg atcgagccca ccggcggcag
      781 ccggcgctgg ctcagcgccg cacgcgagct gcgtcgccgt tacgcgcgcg agcgattcga
      841 cctcgtccat gcccaccacg gtctggccgg gtggagcgca ctggccgtcc gcggcgctcc
      901 gcacctcgtg acgttccacg ggaccgacct cagccaccgg gtggtcgggc cgatgtcacg
      961 cgcgctcgcg cggctgatcg acgcggccgc gcccgtctcg accacgctca cgcgcttcgc
     1021 gggaggcggc ctccctggag cgggccgctc gctgcgtgcg ccggtgctcc cgatgggcgt
     1081 gaatctcgac cgcttcggac gtcgcgaccg cgccgaggcc cgtgctcgcc tcggtctcga
     1141 cccctcccgc ccctacctcc tgttcccggc cgacccggcg cggcccgaga agcgccacga
     1201 ccgcgcacgc gagctggccg cggcggctgg ggccgagctg atcggctacg agggcgtggc
     1261 gccgaaggac gtgcccgatc tgatcaacgc ggtgaacacg gtcgtggcgc cctcggagcg
     1321 agaggggtac gggctggcgc cgctggaggc gctcgcctgc gacgtgccgg tcctctcgac
     1381 cgacgtcgga atcgcgccgc tcgcgctgcg gggggtcgag ggaacactgt gcgcgccctt
     1441 cgaccgcgac cgctggctgc gcgcggtgca gccgcacgtc gccgatccgg atccgcgcat
     1501 cgacggccgg cgccgcgcgg agctgttcga tcgcaaccgt atggctgaac gcgtgttcga
     1561 ggcctatcgg gagctaacct cgatgcgtcc gtgatgaaag gtttccgcaa tcgtgcagcc
     1621 gaaaaggcac gccgcgccgc cagtggcggc gaggggcctc ccgcgggtca ggatccggcg
     1681 gcgcccgggg ccgcgcccgc agcttcgacg gccgatcgcg gcgcgatgcg caagcgcctg
     1741 cgccgcgcgc gccatgcgcg tgacgccgcg ctccacgagc tcggtgcgct cgtgatggag
     1801 atgcaacggc agggtcgcca cgatccgtcg ctcgtggagc gcaaggcgcg cgaggccgtg
     1861 gccgtggacg ccgaggcgcg tgcgctcgcc ggcgcgctcg atcgtggcga ggcgctcacg
     1921 agcgtgagcg cgaccggcat cgtcgggccg tgcggctcgt gcggtgccct ggtcgcgcgc
     1981 gaggaccggt tctgtgggcg ctgcggcacg gcggtcggcg cggcgggcgt gaacggggcg
     2041 acggcggtgg tcggagcggc tgcgccctcg ggcgcccagc gtgtcggggc accgaccgtc
     2101 gcggtcgcgc agcccgtgga cgccgccccg ccttcggccg cgcaacgcgt ggacgcggcc
     2161 ccgcccgcga ccgcggagcg cgtggacgcc gccccgcccc cgtcgggcgc gcagcgtgtg
     2221 gaagccgccc cgcccgcggc cgcggatcgc gtcgagacgg gtccgccgtc ggacgacggt
     2281 gtcccgacct acacggtcac gacccgctga tgcgcttccg cagagacaag gacggcggcg
     2341 ggagcgcggg tccgccggac gcgggcacgc gtgtcgatca gccgaccgag accatcaccg
     2401 ccccgccccc gagcgtggcg gaccgggtcg acgagcccgc ggcgcagccg ccgggcgcgg
     2461 atggtgccga ggagcccacg gccgtgcagc ccacgcgcga gtccgagggc ccggcgaagg
     2521 tgctgccgcc gaccgagggc gaggcgagcg cgcggccgcc gtccccgccg ccgccgagcg
     2581 cgaccgaccg cgtcgaagcc ccggcgcccg ccgcgccgag cgcgaccgac cgagtggagt
     2641 ccccggcgcc gcccgccgcg ccgagcgcga cggaccgagt ggagtccccg gcgcccgccg
     2701 cgccgagcgc gaccgaccgc gtcgagtccc cggcgccgcc cgccgcgcag gccacccgcg
     2761 agtccgaggg cccggcgaag gtgctcgcgc cgccacccac cgcctccacg cgcgtcgacg
     2821 caccagccgc cgcgccgccc ccgcaacccg gccagctcgt ctccgcaaca gcgcccaccc
     2881 aagccggcca gaccgcctgc ccccagtgcg gcacgcccgc cgacgccggc caggagatgt
     2941 gcatcgtctg cggctcgcgc atcgcgcgca agcagtcgaa gccgcgcggc tggctcctcc
     3001 cggccgtgct cgccggcctc ggtgtgctgc tgctcggcac ggcggtcgcg ttcggcgtcg
     3061 tcgagctgac caaggacgac gaggtgaaga agagcgacgt cgccgacctc aacgaggaca
     3121 cccagccctc gaccccaccg ccgtccaccc ggccgccccc cgccgcgacc cctccgacac
     3181 agacctcgcc caccatcccc ccggccacgc gcgagcccga caatccgtcc gggacgcccg
     3241 acacgaccca gaccaccccc gcacccacca cacccggcgg cagcggcaac gagatcgccg
     3301 aatggcccgc cgggaagacg gcctacacag tcgtcctcgt ctcggccacc tcccgcaacg
     3361 cggccgacgc caaggccgag caggcgatcg cccgcggcat caaggcgggc gtgctccgct
     3421 cggacgactt cgcgaccctc cgagccggct actgggtcgt cttcgccggc cagtacgact
     3481 cgtccgagca ggccacccgc gcggccgatc gctacgccga gcagggcttc ggcggcggct
     3541 acccgcgcca agtcaagccg aagtagccga tattcctgac ttaagggtct atatgaccca
     3601 aaagtcagga atgtcgaaga aaaagtcagg ccgagagcgg ccagtagcgc ccgacgaccg
     3661 cggccgcttc ctcactgatg ccgttggcgg caacccgctg gggttcgccg ccaagagaga
     3721 acgcctcgag cgtcgccgcc accgacgtcg ccagcgcgct caggtcgtag ccgccctcca
     3781 gcaccgcgcc caccggcgcg ccgcacgact cgccgagcga ccgcatccag agcgccatcg
     3841 cggcgaacga gtcggtcgac aagcggcagt cggcgagcgg gtcgtcgcgg tgcgcgtcga
     3901 agccggccga gaccaacacg agctccggct cgaacgcccg cgccgccggg accaccacgt
     3961 gctcgaccag agccagccac tcgttctcgc ccgacccggg cggcaccggc aggttcagcg
     4021 agaagccctc gccggcgccg gagcccacgt cgctcagcgg cccggtcccg ggatacagcg
     4081 gcgattggtg gatcgaggcg aacagcacgt cggcggacga atggaagatg tcgttcgtcc
     4141 cgttcccgtg gtggacgtcc cagtcgaata cgaaaacccg ccgcaccccg agcgagtcca
     4201 gcgcgtgccg cgccgccacg gccacgttgt tgaacaggca gaaccccatc gcgcgcgcgg
     4261 actccgcgtg gtgccccggc ggccgcagcc cgcagaagcc gaaccgagcc tcgcccgcca
     4321 cgagcgcctc caccagcgcg cacgccccgc ccgccccgtg cagagccgcg cgatacgacc
     4381 ccgggctggc caccgtgtcg gggtcgaacg cccctccccc tccctccgac atcgcacgga
     4441 ccgactccac gtaccgcacg gggtgcacgg cacccagcac gtcgagcgaa accgcgggcg
     4501 catccctcac cgagtacccg agccagtcgc gcgcctcgag ctcgcgctcg atcgccggga
     4561 tccggcccgg cccctccggg tgcgccccgg tgtcgtgctc cagcgaggac tcgttgcgga
     4621 agtagagcgg cgccgcgatg ccggtaccgt agtcaccgct tgagcaccga gccgacagcg
     4681 tcttacagag cggacgtggc ggtggtgggc gcgggggcag ccggcctcta cgcggcgctc
     4741 gtggccgcgc gcgagggggc ccgcgtcgtg ctcgtctcgc gctcgccgct ggccgagtcc
     4801 gcgagctact gggcccaggg cgggatcgcc gccgcgctgg ccgtctacga ctcccccgag
     4861 ggtcacctcg cggacacgct cgccgccggc cgcgggtcgt gccggcgcag cgccgctcag
     4921 gtgctctgcg acgagtcgcc ggccgccgtg cgcgacctcg agcggctcgg cgtgcacttc
     4981 gacgcggacc gccggggctc gctcgccctc ggcctcgagg gaggccactc cgtgcggcgc
     5041 gtggtgcacg ccggcggcag cgccaccggg cgccggatca cgcgcgagct ctcggcgctc
     5101 gccgcccagg acgagcggat cgagatcctc gagggcacca ccgccagcgc gctctggtgc
     5161 gcggacggcc gctgcgcggg cctgctcgcc cagcgcacga gcggcgcgcg catcgcgctg
     5221 agcgcccgcg cgacgatcct cgccaccggc ggcgccgccg cgctctggga gcgcaccacc
     5281 aacccgcgcg gcgcgatcgg cgccggcctg accctcgccg agcacggcgg cgcgaagctg
     5341 gccgatctcg agatgacgca gttccacccg accgcgctcg tacacgacga cccgcagcac
     5401 gacggcttcc tgatcaccga ggcgatccgc ggcgagggcg ccctgctgct cgacgctcgc
     5461 ggcgagcgct tcgtggacga gctcgccccg cgcgaccagg tggcgctcgc cgtccagggt
     5521 gtgctcgcgc agggcgagca ggtgcacctc gacatgcgcg cgatcgacat gcgcggcttc
     5581 cccaacgtcg cgagcgccct gcggcgagcc ggcttcgacc ccacccggca gctcgtgccc
     5641 gtggcccccg ccgcgcacta cacgatgggc gggatcgcgg tcgacctcga cggccgctcc
     5701 tcgatcgagg gcctcctggc cgtcggcgag tgcgcgtgca gcggcctgca cggagcgaac
     5761 cggctcgcgt cgaactcgct ggcggagtgc ttcgtgttcg gccgccgcgc cggcctggcg
     5821 gccgcggcca gcgacccgct gcccggcgac gtgggcgagc cgcccgcgag cggccccgac
     5881 ccggtcccgc cgcgcccgac gcgcgcggcg ctctggcgcg gcgccggcct gcaccgcacg
     5941 gccgagggcc tgcgctcgct gctcgacgac ccgttcccgc tcgcccggcg catcgccgcc
     6001 gccgcgctcg cgcgcgagga gagccgcggc gcccaccagc gccaggactt ccgcgtcgcc
     6061 gacccccgcc tcgacggcat gcacgtgacg ctcagcggcg acgtcgcgcc gcagctcgag
     6121 cgctggctgt agcgcgccag accgctctct caccggaacg aacgcagctt gtgtgggtta
     6181 acacaacgtt aatccggcgt tctttgtgca ttagcacacc agtggaatac tcggcgccgc
     6241 cttgcaggac tgcggggcat tcgaaggggg gaagggagac catgtaccag ttcagtcgcg
     6301 ccgtctatcg cgagttgagt ccgctcgtcg agatagaccc gcacggatgc gatcgggagc
     6361 accggcgcgc ggtactgacc gcctgtgagc aggccttcac gaggctcgcc accgacaggc
     6421 actacttcgc gcggcccacg cggtcgctgt tcaacgacgt ccgctgctac ttcccgatgg
     6481 cgcggcagat ggacgtctac cgggtgatcg accgctacat gcacatcgcg ggcgactacg
     6541 tggtccagcg cgagcgcgag gggctcagca tcgacggcag cccgctgtgc tgccacgcca
     6601 gcacccgcaa gggcaccgcc tgccagcgcg tgccgctgcc gggcagcaag tactgcccgt
     6661 cgcacaagca cctcgaggag ggcttcgacc cgctcgaaga ggagctcgta accgccgcgt
     6721 agcatccagc gggtgcagat cgcgatcccg ctgttcgatg gtctgaccgc gctcgacgcc
     6781 gtcggtccct acgaggtgct gagccgcccg cccgggtggg acgtggtgtt cgtcgcggcc
     6841 gagaccggcc cgaagcgaac cgacaacgcc gtgctcgcgc tcaccgccga cgcgtcgctg
     6901 gccgacctgc cgcggccgga catcgtggtg gtgcccggcg gaatcggcac ccgctcgagc
     6961 gcgaacgacg acgtggtcga gtggatccgc tcggtccacg agcacacgcg ctggacgaca
     7021 tccgtgtgca cggggtcgct gctgctcggc aaggccggcg tgctggaggg cctgcgcgcg
     7081 accacccact ggctgtcgct cgagatgctg cgcgagtacg gcgccgagcc cacgtcggag
     7141 cgggtggtcg agcagggcaa ggtcatcacc gcggcgggtg tgtcgtcggg gatcgacatg
     7201 gcgctgcgcc tgcttcaggt cgagctcggc gacgagatcg cacaggccgt gcagctctcg
     7261 atcgagtacg acccgcagcc gccgttcgac gccggctcgc cggagaaggc gccgcccgcg
     7321 atcgtcgact tcgttcgcca ggcctccgcg cacctgaagc agtagtgctg ctcggcgtcg
     7381 acgtcggagg cacgttcacc gacgccgcgc tgctggacgg cgagcggctc gtgaccgcga
     7441 aggcaccgac gaccccgcgc gaccagtccg agggcgtgat cgccgccgtc gaggcggcgc
     7501 tcgagcgggc cggggccgag gcgagcgcgg tcgagcgctt cgtgcacggc atgaccgtgg
     7561 ggacgaacgc gctgctcgag ggaaaggtcg cacgcacggc gctgctcgcc accgaggggt
     7621 tcaccgacct cgaggcgctc ggccgccagg cgcgcgccga cctctaccgc ctctgcgcgg
     7681 gacacccgcc gccgctcgtg ccggacgagc tgcgcgtggc ggtgcccgag cgcacgggtc
     7741 ccgacggggt gctgcgcgag ctcgacgagg agctgctgcg cgagcgcctc gacggcgtcg
     7801 acgccgaggc ggccgcggtc tgcctgctgt ggggcttccg gcaccccgag cacgagcggc
     7861 gcgtggcgga gctcgtcgcc gacggcgtgc acgtctccac ctcgcacgag accgcgggcg
     7921 tgttccgcga gtacgagcgc tgcgcgacca cggtggtgga cgcggcgctc tctccgctgc
     7981 tcaggcgcta cctggagcgg ctctccgacc gcgcgcgcga cgccgggctg ccagcgcccg
     8041 aggtgatgct gtcgggcggc ggcgtggtgg ccgcggcgac cgccgcgcgg cacggatcct
     8101 ggacggtgct gtcggggccc gcgggcgggg cggtcgggtg cgcccggctg gccgaccgcg
     8161 ctggccggcc cgacgcggtg gggctcgaca tgggcgggac gtccgcggac gtgtcggtgg
     8221 cgagcggcgg gcgcgcggcg tccaccggcg ggcgcgaggt cggcgggcgc gcgctggcgc
     8281 tgccgatggt cgacgtccac acggtcggcg ccggcggcgg gagcgtggcc tggcgcgacg
     8341 ccggcggagc gctgcgcgtg ggcccgcgct cggcgggcgc cgacccgggg ccggccgcct
     8401 acggccgcgg cggcaccgaa ccgaccgtga cggacgcgaa cctcgtgctc ggctacctcg
     8461 atgccggctc gccgctcgcc ggcggggttt cgctcgacgc cggcgccgcc gcgcgcgtgg
     8521 tgggcgagct cggcgactcg ctcggcctgt ccctcgagga gaccgccgcg gggatcgtgc
     8581 gggtggcggg agtcgagatg gcgcgggccg tccgggtgat gaccgtggag cgcggcatcg
     8641 acccgcgcga gctggcgctg ctcgccttcg gcggggccgg cccgctgcac gccgcggcgg
     8701 ttgccgagga gctcggcatg cggcgcgtgc tcgtgccgcg ctcgtccggg gtgctgtcgg
     8761 ccgtgggcct gatcgcctcc gagcgccgcc gcgacctggt cgagagcgtg ctgctggcgg
     8821 gcgaggcgct cgcgcgcgag accgtcgccg gtgcggtcga gcgcctggcc gcgcgcgggc
     8881 gcgaggagct cggggagccg gacgccga
//
LOCUS       DASVPL010000222        28096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_26470, whole genome shotgun sequence.
ACCESSION   DASVPL010000222 DASVPL010000000
VERSION     DASVPL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 28096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28096
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26470"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VF517_11870"
     CDS             complement(<1..963)
                     /locus_tag="VF517_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8053679.1"
                     /translation="MSDQNNAQAEGPSANAQAAELEFRAATKRYPGQAEAAVNALSLK
                     VPAGEICVLVGPSGCGKTTAMRMVNRMIDITEGDILLDGRSVRDRDPADLRRDIGYAI
                     QQIGLFPHQTVGQNIATVPRLLGWDKSRIRARVDELLELVSLDPHETRDRYPAQLSGG
                     QRQRVGVARALAADPPLMLMDEPFGAIDPINRERLQNEFLRLQKEIRKTIVFVTHDID
                     EAIKMGDKIAVLKKGGHLAQYASPAELLTAPADRFVEDFVGADRALKRLALQRVRDID
                     LWRAPLVRVGEPAAEARAKVADSDLPYPLLVDDEGRPLGWLSDRA"
     gene            complement(1006..1653)
                     /locus_tag="VF517_11875"
     CDS             complement(1006..1653)
                     /locus_tag="VF517_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8053680.1"
                     /translation="MSGLAGGAAALQAGRREERKAKNRAKLLAAARRVFAEKGFGEAT
                     ARDIVRETDLATGTFYNYFRDKDELFRALLEEFQQRVSDATLPLRRDANLSLEERIAQ
                     GALAFFQVVAEDAELFHVLRRNAGAIAILPSESMFPEGMAELDEDLDQWRLDGDLPCI
                     DLDYLSAAIAGAAFQIANRMVERDPPDPEEAARFTARAVIGVIHSVAPDSTSQAR"
     gene            complement(1677..2642)
                     /locus_tag="VF517_11880"
     CDS             complement(1677..2642)
                     /locus_tag="VF517_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX8053681.1"
                     /translation="MLVTIAAVKAVTIRDEALSVEEHDDPVPGTGELLIRVRAAGLNG
                     ADMLQRRGLYPAPPGAPQDIPGLELAGEVAALGPGATRFAEGDRVMAVVAGGGQAELA
                     TVHERAAMPVPDALDWPAAGGFPEVFTTAHDALFTQAGLGAGEHLLVHGAAGGVGTAA
                     VQIARAAGARVTGTVRREEMRGAVEELGASAIDPEGFEDHGPFDVVLELVGAPNMAGN
                     LKALATGGRIVTIGIGAGAKAELNLGLLMAKRAVLRGSTLRARPLEQKADAARRVEHA
                     VLPLVESGAVKVPVADTFPLADASSAYERFAAGGKLGKVVVRVPD"
     gene            2842..3555
                     /locus_tag="VF517_11885"
     CDS             2842..3555
                     /locus_tag="VF517_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053682.1"
                     /translation="MLLIPGFLAGDDSLGIMTRWLRRTGHHTRKAGMRSNIDCSAKAY
                     DLLEERLETMAESRGEKVAIVGQSRGGTFAKVLANRRPDLVSGIVTLGSPQRYPLAVH
                     PLVRANVYALGAIGSLGAPGLFRHTCLWGDCCKSFRDDLLSELPAEVGYVSIYSRTDG
                     IVDWRACLDEGAVHVEVRASHCGMAVNAGAYEALAAALADFRARGREPGNRRRRGTAA
                     RATQPSGRTAKPSRLSRAA"
     gene            complement(3552..3863)
                     /locus_tag="VF517_11890"
     CDS             complement(3552..3863)
                     /locus_tag="VF517_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8053683.1"
                     /translation="MKLYVCYGTWKAAPRPGGHPCGTAYHALREAGHDPEVIKSYGLM
                     ILPDTPFNQTAGRKEAKRLTGSSTVPVLVTDDGEVVSDSRKIADWAKKNPAGAAAATA
                     Y"
     gene            3914..4246
                     /locus_tag="VF517_11895"
     CDS             3914..4246
                     /locus_tag="VF517_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053684.1"
                     /translation="MRTHPVKEFAAEAARTVGRRVRARRRGHHADAAPAAPAPRSIPL
                     PQVPLNAAKQPANGALDARGSAGADELHAPEAAADDGELDALRGALVRELDRLAADDD
                     CSASFRRA"
     gene            complement(4259..5806)
                     /locus_tag="VF517_11900"
     CDS             complement(4259..5806)
                     /locus_tag="VF517_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053685.1"
                     /translation="MPSLANLRPARSRALVAATVAALALPALGGTAAASTSQESIMQD
                     DHVLYNEGPAAQARALDQMAALGVDTIHTVISWRGLAPDNAALKPPKGVKLDDPRSYD
                     PVRWNTYDDLVRGAAARGMQVLMTPSGPAPRWAEGCSESEREKYNPGTCRPSPGKLGQ
                     FVEALARRYSGRYTDESGEGKLPAVKRWSFWNEPNLNSWLSPQTARVKGRTIQKGAAM
                     YRSIVHAGNAGLRAGGHRRDQVLLGESAPIAGGSRGAAPVTFYRALFCVDSRGRKLKG
                     SAASAIGCSKPRRFAVGGIAHHPYTRGAGDPLTTRQSSANITIAYTSRLKAVARQGEH
                     SGLVPRGISSKIYFTEFGVSSNPPGTRYSVPLDTQAEWINQADYMAYRDSGVRSVAQF
                     ALEDDPSFGRDSFQTGICFYNQPNPCYPKPAYAAYRVPIYVVERGRNVLIYGQARPAA
                     ANQRRLEIQNRADANAPWQTVQTVDLSTRGQFLKTLPARPGTWRLAWTPTPGTTHLSR
                     EAVARKR"
     gene            5883..6863
                     /gene="mtnA"
                     /locus_tag="VF517_11905"
     CDS             5883..6863
                     /gene="mtnA"
                     /locus_tag="VF517_11905"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX8053686.1"
                     /translation="MAAISPICWRDGVLELLDQRCLPGEEAWLRCSATCQVADAIRSL
                     AVRGAPAIGIAAAYGLALAAARGEDLAEAARMLGDTRPTAVNLRWALNRTLAAGREGA
                     LAEAHRIFAEQVEADRRMGELGAELLAAGDRVLTHCNAGALATGGRGTAVGVLRSAWE
                     TGRLAHVWVGETRPLLQGARLTAWELEREGIPYRVVADSGAGSLMARGLVSRVIVGAD
                     RIARNGDVANKVGTYPLAVLADRHLIPFYVAAPVSTIDHATPSGDAIEIEEREPAEVV
                     ELGGRRIAPPGAEAANPAFDVTPAELVTAIVTEAGVLEPPYEEAIAGVLG"
     gene            6850..7881
                     /locus_tag="VF517_11910"
     CDS             6850..7881
                     /locus_tag="VF517_11910"
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HEX8053687.1"
                     /translation="MSSADRGRRGGEPRPATPGEARRPALDADALLGAARRPLVVGIG
                     GGGDVVGALATAEAARLQHGARPVVGGVTWERRPIDPVPGPRHLNEIEGGRQLAPGVL
                     AAGPDTRVRESGVLFGESRMAGFLGEETVLVDANGGPAAVAAGLTAAVDALGADLVVF
                     LDVGGDVLAHGDEPGLASPLCDSILLAAAGRMAAAGVPTLGGLFGIGCDGELTPREVD
                     ARLAEIGAAGGLAGARGLTEPVAALLEGAIEHVPTEASAMAVRAFRGQTGPTAIRAGR
                     RTLELTMAAAATVYFDVTVAMATAARLPALVDPATSLDEANDALNAAGIRTELDYERE
                     NAYPRAPGA"
     gene            complement(7878..8324)
                     /locus_tag="VF517_11915"
     CDS             complement(7878..8324)
                     /locus_tag="VF517_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053688.1"
                     /translation="MAPEPAARGLSLSILRKPTALLLIVVMAVGSVFLWLGIPFLWIY
                     GVSQMVDTTAPQLWPLLAILFGVPVSMWLFGRFLYRMNGAYERVTGQTSDVRVQLPWH
                     RSMRGERDVGRRTTVLEFVMICSVGLCLLAFGIWFLFFAGSSLPGG"
     gene            8383..9525
                     /locus_tag="VF517_11920"
     CDS             8383..9525
                     /locus_tag="VF517_11920"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX8053689.1"
                     /translation="MKLGVPNQGSAGESRVATVPDVVRRLSKKGVEVVVETGAGARSH
                     VPDEEFEAAGATIGSAEQTWGADAVAFVKAPGAAEIERLRRGAVVMGFLNPLTDTETA
                     SALASAGVTSFAMEAIPRITRAQSMDALSSQSTVSGYRAVLVAAEELDRFFPMLTTAA
                     GTIKPATVLVLGAGVAGLQAIATARRLGAVVTGYDVRAVVREQVESLGARFLELDLET
                     DAEGAGGYAKALDEDQQRRQQEAMAEAAGKFDVVITTALIPGRPAPLLLIAKAVENMR
                     PGSVVVDLAAVTGGNCELTEPGQTVERHDVKILGPLDLAAEMPTHASQLYARNIQSLV
                     ELMVGEEGALELNWEDEIIAGACITRDGEIVHEGAKKAAGAPASQA"
     gene            9534..9872
                     /locus_tag="VF517_11925"
     CDS             9534..9872
                     /locus_tag="VF517_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX8053690.1"
                     /translation="MDIITMIAILVLAAFVGFEVISKVPNTLHTPLMSGTNAIHGIVL
                     LGGLLVISRADGVLDKALLTIAIAFGTINVVGGFLVTDRMLGMFKGREKKKEPAAADA
                     ASGDGSGGGS"
     gene            9869..11296
                     /locus_tag="VF517_11930"
     CDS             9869..11296
                     /locus_tag="VF517_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX8053691.1"
                     /translation="MTTLATSFVQDRDFINALYIVAFVLFIIGLRGLGGPKTAVRGNK
                     IAAVGMLIAFVATLLIDDVIADSEDAILIAVGVIAGTAIGVPAARSVKMTAMPQMVAL
                     FNGVGGGAVALIAWAEFRHGGADLELKVLIPILIAAIIGSISFWGSNIAFGKLQEILP
                     GRPISLPGQSIINLLLALVTVGTAIAIAAGTDSEALFVLGILVAGALLGNLMVLPIGG
                     ADMPVVISLLNAFTGVSAALAGIALENTALIVAGIVVGASGSILTNMMAVAMNRSVPA
                     IIAGGFGGVTTAPTADGDEDRTVRSTSADDVAVQLAYADRVVVVPGYGMAVAQAQHTV
                     RELADELEQNHGVEVSFAIHPVAGRMPGHMNVLLAEADVEYDKLKEMDEINPDFPQTD
                     VSLVIGANDVTNPDARTNEGSPIYGMPILDVDKSGQVIVLKRSMNTGFAGIDNPLFYA
                     ENTALLFGDAKKSVQQVLGEVKELG"
     gene            11388..12038
                     /locus_tag="VF517_11935"
     CDS             11388..12038
                     /locus_tag="VF517_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053692.1"
                     /translation="MEFEESRIEDSLVRAPDGDVVALGGYCESLGISRERVPADPVRL
                     LSYGANASPDELARKLAGHDPVVPVALTEIGDLDVVFSAHASPRGGLGAAVQRSPGTW
                     IEVAITYLHPDLLPLIDATEENYRRVAMPSRGVEFYVSRHGCLVLDGSEVAMAAVRAR
                     GRRFPAMTTLEAVEAVRGLLAPDVPLDRFVHENATDAELRAQRTEVLRRGAKPFGP"
     gene            complement(12019..12789)
                     /locus_tag="VF517_11940"
     CDS             complement(12019..12789)
                     /locus_tag="VF517_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8053693.1"
                     /translation="MDFDSADLAQRLDAVYRTRDVVRRRRLVREAVAAAPGERILDVG
                     CGPGYYVAELAEEVGPDGTVTGIDQSPAMLAVARDRCKSLPGTSFGEGEATALPFDDA
                     SFDAVLSVQVMEYVPDVAAALAEVHRVLRPGGRVVLWDIDWTTVSWHSRDPVRMRRVL
                     EAWDGHLIDPALPRRLAPLMRAGGFEDVRMEAHTFATAELTLDAYGGSLIGVVEPFVA
                     ASGLADEASAWAAEQRELGESGEFYFAVVQVCFTGRRA"
     gene            12819..13994
                     /locus_tag="VF517_11945"
     CDS             12819..13994
                     /locus_tag="VF517_11945"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8053694.1"
                     /translation="MERHEVLVIGAGPAGLATAAVLERAGIPAPVLEKSDNVAASWRR
                     HYDRLHLHTVRWLSNLPGYRFSRKHGRWVSRDGVVRYLEGYVRHHELDVRTGVEVQRL
                     ERDDAADEWVLDTPGGELRARHVVVATGYNHTPVMPDWPGRDGFEGTLIHGQDYRNPE
                     PYRGQDVLVVGSGNTGAEIAVDLVEGGAARVRIAVRTPPAVMLREANGLPSQITGVMM
                     RRLPLPVADALSRSSARMLIGDLSEYGLPPVERPITRAVKEDVIPILDVGLVELVKEG
                     RVEVVAGVVSFEGRDVVLADGSRVQPDAVISCAGYARGLEPLVGHLGLLGHKGRPTVH
                     GGETHPNAPRLYFIGYTNPISGMFREFRIVAPKIAKAIARDRGAPRPAGREREPAAA"
     gene            complement(14048..14491)
                     /locus_tag="VF517_11950"
     CDS             complement(14048..14491)
                     /locus_tag="VF517_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8053695.1"
                     /translation="MATASKTASPRSSPAAEAWGLLLQLFFAHHRPRLIELGQELDLA
                     PQQLLALKMLDEPRPMSALAGALMCDNSNVTGIVDRLETRGLVERQPSPLDRRVKLLV
                     LTGEGRRVRELITARMTSPPPPIAELSAKDQRELRDLLRRALGGA"
     gene            14560..15546
                     /locus_tag="VF517_11955"
     CDS             14560..15546
                     /locus_tag="VF517_11955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8053696.1"
                     /translation="MTQPEGIEVEGLVREFKKGPRAVDGIDLEVAPGEIYGFLGPNGA
                     GKSTTVHMLTTLLPPTAGTARVGGFDVVKQGPQVRSTIGAALQDAALDPLMTATEHMK
                     LQTALHGLPKAERDRRGRDLIERVGLSEAADRRVKGYSGGMKRRLDLALALVHEPRIL
                     FLDEPTTGLDPQSRAALWDEVARLANEDGVTVFLTTQYLEEADVLANRVGIIDNGRIV
                     AEGTPTELKAQIGRPTVEAIPRDPDQREAAQEVLRRFGEDFNEASSRGAAVRLTRGVD
                     ELASIVRALDDDGIALANIELHHPTLDDVFLAKTGRSLEGAEEDEVEEPVAA"
     gene            15543..16355
                     /locus_tag="VF517_11960"
     CDS             15543..16355
                     /locus_tag="VF517_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8053697.1"
                     /translation="MSASLAMPRQAFRTPLAIQVGYIARRSVIRTLRQPAIIVPGVTF
                     PLFLLAVNAGGLDAATHLPGFPTDSYLTFALAITFMQAALFATNAGGNDLAEDIRTGF
                     LSRLSLTPLRGSALLAGQLAGIVALGLFQAVVFISVGLAAGGKIEAGAPGVLVLIALS
                     VLTGLAFGTVGMLIGLRTGSGEAVQGFFPLLFVMLFLSSMSLPRDLIEQDWFRTIATY
                     NPVSYLIEGFRSLLIYGWDGEALALGFGVATTIFVVALALSARALQTRMVRT"
     gene            16352..17116
                     /locus_tag="VF517_11965"
     CDS             16352..17116
                     /locus_tag="VF517_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8053698.1"
                     /translation="MSYYSVARAVAWRHLNNLVKNPALFLPPLIFPLFFFTAFAGGLS
                     AVGDAPGFDYPDYTAFQFVFVMLQTASFGGVFTGFSIAADWESGFARRMMLAAPHRSA
                     LIAGYAAAALLRTLLVGGMVFLVALVTGMEIGGNGIELGGLLGLALILNVAATLFGAG
                     VSLRTRTMQAAPMLQTPVFLFLFLAPVYAPRDLLAGWIHSVADVNPATALLEAGRALI
                     AGLPAEAGLAYAVATGLAVLFAVWAVRGMRSAENAV"
     gene            17169..19316
                     /locus_tag="VF517_11970"
     CDS             17169..19316
                     /locus_tag="VF517_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX8053699.1"
                     /translation="MRVPLLAIAVAFLAVPASAGAATYDVTTTADNLTCNPGNCSLRG
                     AIEAANAASGADTINLPAGNYVLELSGGDDDNAVGDLDVNGSLTLNGAGAGTTTVDAG
                     DTEDRVFDVGPNGGDALVVALRDLTVRDGNADFEGGGVRSSAQTTLQRVVVTGSAAFR
                     GGGVAQTSSTTLLRILDSTITGNSGQQGGGVAHDAGGHSDTGAVGVLIERSTISDNHA
                     VPGGEGDPDGGGIAVNLDGDVRVRSSTISGNSAEGFQDASGGGAYVNGGLLEVTNSTV
                     SGNSVTGGYGGGIANTDGDLTVLNATIAGNSSTAGGVTSRRIAAAAVQNPGDPSQPGG
                     NVDSFGDSNTFKNTIVAGGSPQNCRGRFNTVFTSSGHNLEDANTCGFDATGDAVNTDP
                     QLGPLAANGGPTRTRALPKSSPAIDTADNAACPATDQRGIARAQPAGGTCDKGAYERP
                     GLDLSITKLASAATVKVGEEVRFTLAVSNSGPADAGGVRVTDALPDGLQFVSADPSRG
                     SCSGTTTVTCELGTLAAEQDADVVIRATATRAGEFTNTAAVASNEDFPEPNRANNSAS
                     VSVRAEDVAQGCRDTVAPLTELKQSRVRVRRGKVRLSGVSYDPDPCASGVNRVLVSLA
                     RVKGRHLVNCRFLRSMTRYLLTPAPGMNCQDPVLFRAKGGSSQGTARTSYSFIYFVDL
                     PDGIYRAQARAFDNAGNKERPTKGRNIRKFFVR"
     gene            19358..20062
                     /locus_tag="VF517_11975"
     CDS             19358..20062
                     /locus_tag="VF517_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053700.1"
                     /translation="MAEEQSTPQSDLPRDDDRTDKQQESASGGGDSPEDKAEQDQGED
                     TEATAGASGEGQSDAMEAAEKAKEEQEEAKEKMKELEEDPPEKLEDWPDDKTKYETFG
                     GAEGNESYDDSVTAKLGPSSLRHREDGSVEVEGEEVDDPEEYKGDPIPGGPTDPQAAE
                     SRAYGEPDLTDETSQDVEGSGDGGEGSGSGEGGEEDTSEGTSEGTSEGTSEGTSEGTS
                     SSSSDGEDDDGDDEKS"
     gene            complement(20059..22173)
                     /locus_tag="VF517_11980"
     CDS             complement(20059..22173)
                     /locus_tag="VF517_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8053701.1"
                     /translation="MRVDESVVIDATIEEVWEAVGDLPRWDRLLAAATRWEHAGGPER
                     GLGARYQLRLHVGSAHVGSLIEVVECDAPGDLAWTSITGLDQRGRWRLRETDDGRTRV
                     WLRLSYQAPGGVLGMVADRASSPMVGDNLRETLERLKREVEGERYMGDGSGNDGGLVS
                     SVTAGVEQALETVRTVWSAGLVRPERPDRLARALIAIQRWGYTPATGYIANAARYPDQ
                     DAVIDDLGRLTFAEVHERTNRLASGLSDAGVLEGDGVAIMCRNHRYFVETVVACSKLG
                     AHALFLNTAFAGPQLTEVVKREKPVAVVYDEEFAELLADAGKRRKRFVAWYEGEDPPD
                     PTLEDLIQDGDPADVVPPEEQGRVVILTSGTTGTPKGASRKQAEGIGPAISMLSRIPL
                     HAGDKALIAAPLFHSWGFAHFTLGMALHHTIVLQRRFKPESTLAGIAQHQVQSCPMVP
                     VMVQRIMDLPEEVRRKYDTSSLRSIPLSGSALPGDLAIEFMNEFGDILFNLYGSTEVA
                     WATIATPDDLRAAPGTAGKPPMGTTLRIYDENGNQVPPGGEGRIFVANDMLFEGYTGG
                     GSKDVIDGLMSTGDVGYIDDEGRLFVSGRDDEMIVSGGENVFPREVEDTISNMQGVSE
                     VAVIGVDDEEWGQRLKAFVVKKGSGVSEDEVKKHVKSNLAGYKVPREVEFIKELPRNA
                     TGKVLKRELTEAEEEKAEAKKS"
     gene            complement(22170..23102)
                     /locus_tag="VF517_11985"
     CDS             complement(22170..23102)
                     /locus_tag="VF517_11985"
                     /inference="COORDINATES: protein motif:HMM:NF015915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8053702.1"
                     /translation="MSSAREYERSAARRARTNGSVNGSASAGGLASALGRAIAEQVQS
                     RIPTADLDERDPDYIRETLPGLWLLASFYFRADVKGLENIPAEGPVLLVGNHSGGNMT
                     PDTLVLTLAFNTYFGVERRFYQLAHNLVVSMPWLATLRRYGTVAASPQNAKKALDTGA
                     ALLVYPGGDYEVHRPSWESARVDFNGRKGYVRLALEHGVPIVPIVSLGGQETALFLTR
                     GERLARLLRLDRMFRLKVLPISLAAPWGVNVGDMLGHVPLPAKLQIRALEPIDLNEQF
                     GADPDVDEVDEYVLAKMQATLDSLAEQRTLPVIG"
     gene            complement(23128..24207)
                     /locus_tag="VF517_11990"
     CDS             complement(23128..24207)
                     /locus_tag="VF517_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8053703.1"
                     /translation="MSRSPRIGLVLGAGGVMGGAWLTGGLEAIATETGWDPSSADYIV
                     GTSAGSMMGGLLSSGVPPWFMVAHSEGRSFEGLMGVDGRPQDTADRAAGATFRVERAL
                     PRVGPGSWGLIARSVTAPHLHRPSALLAGWLPQGVISNEPLKDTIRRVVPSGWNDHPN
                     FWAVACDYATGRRVPFGRADAPPAELADAVAASCAIPGFYRPVRIGGRRYVDGGLWST
                     SNLDILRGLDLDLVVCLNPTSSLHPPFAWNPVERVAGVMRGMSGRRLGGESKKLRESG
                     TEVVLIQPTAEDHEAMGPNLMSRRNLTVVIRTAIRTVGEQLRADGVRQYLDDLPEGEP
                     HKIREPDGPPSEWPETLPGVRRQAA"
     gene            complement(24224..26974)
                     /locus_tag="VF517_11995"
     CDS             complement(24224..26974)
                     /locus_tag="VF517_11995"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX8053704.1"
                     /translation="MRRLLVIAAVAVTAALAAAPSQAATPVTWDKYSLRIENRPVFVN
                     AGEVHPNRMPGGPAQWRRVLRTLKAAGLDTISIYVFWQDHEIAPGRFRFDGVWDVERF
                     MREARDAGLDVIARIGPYVQGEVDGGGYPWWTLGKPGIHRTGEPEFTADWKRWFAAVL
                     PRLARWQAGGANRGTMVALQVENEHPGGGAEETEQSRAYMRDLVQTAKGLGIEVPITH
                     NDVQLLGQERSRGHFLDLVDVFGFDNYPYGFACCADWNEETFRRQVDTFEDYYRDEVG
                     VQRSPLYAAEIQGGLIAFADDGHTTEDRYRHMIGYEYVQTISLLGQGLTMINRYMTYG
                     GTTWGNQVYPNAGTGYDYGAPVREWGALGTRYDELRRIGLQIRAAGGSIARTERVDAP
                     EGVYRMRRSVDDGTLHVFLRNADPGVERDTPVTIDGRTTPPVPLPGHSARWLLARANL
                     SGWRIDLTSAEVAHADKRFLVLFGDRGRPYSVWIAGRRFDFKPAKHPRILRLGKRTLV
                     IMSRSQAARVWKSGTRLVVGPLLLMGSTVETSRRTRTTTIARGSVKRRTLEGPARVKV
                     PRLSDWRRRAETPERQPAFDDSAWPRLEKLSTHNQVQPNTSPVLGSDDNGVPGSGFIW
                     YRGRYSGPAGELCLEGRHRYHVWLNGRSLGTVTSAAEVPGPMGLGGLGASPPVPQPAR
                     LALPADAQTGGENVIAVLVESWGHNMDAGGAIQAKNPRGLISASLDRPGAPPCGWTLG
                     PAGPGGEFSTPLAAGTYSTAPGLPKPSGGIDWRIHGAAPEDYPNTSGLHGEGAGWHRG
                     GFDDSSWERVSLPRETSSGPGEVAWYRTRFRLSVPRGHYAAFGLDLPKASFPANVYLN
                     GVQIARAGRDREERFYLPAGVLRERGENTLAIARWNVGESARMDAPALHLYERRRVAR
                     LP"
     gene            complement(27085..>28096)
                     /locus_tag="VF517_12000"
     CDS             complement(27085..>28096)
                     /locus_tag="VF517_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053705.1"
                     /translation="GPGPPRTGAAAAVLGGAGRVSTARGSAIAAPLATVSAMSVTVQV
                     GLLFALATAFTSVLGFLLKHRGAGQAPPVDARRPVRSSLALFSARDYTIGIVVATGSW
                     GLHVAALSLAPISLVQSVIAGGLVLLTVLADRLFGQRVTRREWIGVGLTALGLAFLAA
                     TMSGTGDEAHSDYGAGTLALYAGLLAAAGMAAAVYGWGESRARAPFVGVGAGLLWGAS
                     DVSIKALSDSLDNDGVLVLLHPLAFVIASLSLIGLTVSARSLQQGGAVPVIAATSAAA
                     NLVTIAAGPIVFGEPMPDDPLGFTLRLLAFALVIVAASLTPPPGADEEDASAVEPEAA
                     PA"
BASE COUNT         3874 a        10177 c        10181 g         3864 t
ORIGIN      
        1 cgcccggtcg ctcagccatc cgagcgggcg gccctcgtcg tccaccaaca gcgggtaggg
       61 caggtccgag tccgcgacct tcgcgcgggc ctccgccgcc ggctcaccca cgcgcacgag
      121 cggcgcccgc cagaggtcga tgtcgcgcac gcgctggagc gcaagccgct tgagcgcgcg
      181 gtcggcgccc acgaagtcct ccacgaagcg gtcggcgggc gcggtcagca gctcggccgg
      241 gctcgcgtac tgggcgaggt ggccgccctt cttcagcacc gcgatcttgt cgcccatctt
      301 gatcgcctcg tcgatgtcgt gggtgacgaa gacgatcgtc ttgcggatct ccttctggag
      361 gcgcaggaac tcgttctgga ggcgctcgcg gttgatcggg tcgatcgcgc cgaacggctc
      421 gtccatcagc atgagcggcg ggtcggcggc gagggcgcgc gccaccccga cgcgctggcg
      481 ctggccgccc gatagctgtg ccgggtagcg gtcgcgcgtc tcgtgcgggt cgagcgagac
      541 gagctcgagc agctcgtcca cccgggcgcg gatccgcgac ttgtcccagc cgagcaggcg
      601 cgggaccgtg gcgatgttct ggccgacggt ctggtgcggg aacagcccga tctgctggat
      661 cgcgtagccg atgtcgcggc gcaggtccgc cgggtcgcgg tcgcggacgc tgcggccgtc
      721 gagcaggatg tcgccctcgg tgatgtcgat catgcggttg accatccgca tcgcggtggt
      781 cttcccgcag cccgacggcc cgaccaggac gcagatctcg ccggccggaa ccttcagcga
      841 gagcgcgttg accgcggcct cggcctgccc gggataccgc ttggtcgcgg ctcgaaactc
      901 gagctctgcg gcctgtgcgt tcgcggacgg cccctcggcc tgggcgttgt tctgatctga
      961 caaggagggc ttcccgcccg ggcacctgcc cgggacgccg ggatcctacc ttgcctgcga
     1021 ggtggagtcg ggtgcgaccg agtggatcac gccgatcacc gctcgggcgg tgaaccgagc
     1081 cgcctcctcg ggatcgggcg gatcgcgctc aaccatgcgg ttcgcgattt ggaacgccgc
     1141 gccggcgatc gccgccgaga ggtagtcgag gtcgatgcag ggcaggtcgc cgtcgagcct
     1201 ccactggtcg agatcctcgt cgagctcggc catgccctcg gggaacatcg actcgctcgg
     1261 gaggatcgcg atggcgcctg cgttacggcg caggacgtgg aagagctcgg cgtcctcggc
     1321 gacgacctgg aagaacgcga gcgcgccctg ggcgatgcgc tcctcgagcg agaggttcgc
     1381 gtcgcggcgc agcgggaggg tcgcgtcgct cacgcgctgc tggaactcct cgagcagggc
     1441 ccggaacagc tcgtccttgt cgcggaagta gttgtagaag gtgcccgtcg cgagatcggt
     1501 ctcgcggacg atgtcgcggg cggtcgcctc gccaaagccc ttctcggcga agacccgacg
     1561 cgccgccgcc agcagcttcg cccgattctt cgccttgcgc tcctcgcggc gccccgcctg
     1621 caacgccgcc gctccccctg ccaacccgct cactgaaaaa tgttgtaccg caaaactcag
     1681 tcaggcacgc gcactacgac cttcccgagc ttgcctccgg cggcgaagcg ttcgtacgcg
     1741 gaggaggcgt cggccagcgg gaaggtgtct gccacgggca ccttcacggc gcccgactcg
     1801 accagcggga gcacggcgtg ctcgacgcgc ctggcggcgt cggccttctg ctcgagcggg
     1861 cgcgcgcgca gcgtcgagcc gcgcagcacg gcgcgctttg ccatgagcag gccgaggttg
     1921 agctcggcct tcgcgcccgc tccgatgccg atcgtgacga tccggccgcc ggtcgccagc
     1981 gccttcaggt tgccggccat gttgggagcg ccgacgagct ccaggacgac gtcgaacggc
     2041 ccgtggtcct cgaacccctc cgggtcgatc gcgctcgcgc cgagctcctc gaccgcgccg
     2101 cgcatctcct cgcggcggac cgtgccggtc acccgcgcgc cggcggcgcg ggcgatctgg
     2161 acggccgcgg tgccgacgcc gcccgcggcg ccgtgcacga gcaggtgctc gcccgcgccg
     2221 aggccggcct gggtgaagag cgcgtcgtgg gcggtggtga agacctccgg gaagccgccg
     2281 gcggccggcc agtcgagggc gtcgggcacc ggcatcgcgg cccgctcgtg caccgtcgcg
     2341 agctccgcct ggccgccgcc ggcgacgacc gccatgaccc ggtcgccctc cgcgaagcgg
     2401 gtcgcccccg gcccgagcgc ggccacctcg ccggcgagct cgaggcccgg gatgtcctgc
     2461 ggagcccccg gcggcgccgg gtacaggccg cggcgctgga gcatgtcggc gccgttcagg
     2521 cccgcggccc ggacgcggat cagtagctcg ccggtgccag gaacgggatc gtcgtgctcc
     2581 tcgacggaga gcgcctcatc cctgatcgtg acggccttca cggcggcgat cgtaacgagc
     2641 acggactagt gcctacgcgc catcgctttt ttaggagccg atccgtgggg tactttggtc
     2701 ccagaggctc gtatgacccc caggtcactt tttgcgctcc cgcccctgtg gcgagagggg
     2761 aggatcggtc ttgaggccgc cgccctcctg aggcacccga tccatcgagg tgagggcgtc
     2821 gcgcatgccg acggccagcc ggtgctgctg atccccggct tcctcgccgg cgacgactcg
     2881 ctcgggatca tgacccgctg gctgcggcgc accgggcacc acacgcgcaa ggccggcatg
     2941 cgctcgaaca tcgactgctc ggcgaaggcg tacgacctgc tcgaggaacg gctcgagacg
     3001 atggccgaga gccgcggaga gaaggtcgcg atcgtcggtc agagccgcgg cgggaccttc
     3061 gcgaaggtgc ttgccaatcg gcgtcccgac ctcgtgtcgg gcatcgtcac gctcggttcg
     3121 ccgcagcggt atccgctcgc cgtccacccg ctcgtgcgag cgaacgtcta cgcgctgggc
     3181 gcgatcggtt cgctcggcgc gccggggctg ttccggcaca cgtgcctctg gggcgactgc
     3241 tgcaagagct tcagggacga cctgctcagc gagctgccgg cggaggtcgg gtacgtctcg
     3301 atctactcgc gcaccgacgg catcgtggac tggcgtgcat gcctggacga gggcgcggtc
     3361 cacgtcgagg tgcgggccag ccactgcggg atggcggtca acgccggcgc ctacgaggcg
     3421 ctcgccgcag cgctggcgga cttccgcgcc cgcgggcgcg agccgggcaa ccggcggcgc
     3481 cgcggcaccg cggcgcgggc cacgcagcct tcgggccgga cggccaagcc gtcgcggctc
     3541 tcgcgcgcgg cctagtaggc ggtcgcggcg gctgcgccgg ccgggttctt cttcgcccag
     3601 tccgcgatct tccgcgagtc gctgacgacc tcgccgtcgt cggtcacaag caccgggacg
     3661 gtcgagctgc cggtcaggcg cttcgcttcc ttgcggcccg ccgtctggtt gaacggcgtg
     3721 tcgggcagga tcattagccc gtaggacttg atgacctcgg ggtcgtgacc cgcctcgcgc
     3781 agcgcgtggt aggcggtccc gcaggggtga ccgccgggac gcggggcggc cttccatgtt
     3841 ccgtaacaga cgtagagctt catagggcca agttctagcg gttcccgtcc gggggcgcgg
     3901 ccaccatgga ggcatgagga cacatcccgt taaggagttc gccgccgagg cggcccgaac
     3961 ggttggccgc cgggtgcggg cgcgcaggcg cggccaccac gccgacgcgg cgcccgcggc
     4021 ccccgctccg cgctcgatcc cgctccccca ggtgccgctg aacgcggcga agcagcccgc
     4081 caatggcgcg ctcgacgcgc gaggatcggc cggcgccgac gaactccacg cgccggaggc
     4141 ggccgccgac gacggcgagc tcgacgccct gcgcggtgcg ctcgtgcgcg agctggaccg
     4201 cctcgccgcc gacgacgact gctcggcgag cttcaggcgc gcgtagcggg cccggcgtct
     4261 agcgcttgcg cgctaccgct tcgcggctga ggtgcgtcgt gcccggggtc ggcgtccacg
     4321 ccagccgcca ggttcccggc cgcgccggga gggtcttcag gaattggcca cgggtgctca
     4381 ggtccacggt ctggaccgtc tgccagggcg cgttcgcgtc cgcccggttc tggatctcga
     4441 gccggcgctg gttggccgcc gccgggcgcg cctgcccgta gatcagcacg ttgcggccgc
     4501 gctcgaccac gtagatcggg acgcgatagg cggcgtaggc cggcttcggg tagcacgggt
     4561 tcggctggtt gtagaagcag atcccggtct ggaagctgtc gcgcccgaac gaggggtcgt
     4621 cctcgagcgc gaactgggcg accgagcgca cgccggagtc gcggtaggcc atgtagtcgg
     4681 cctggttgat ccactccgcc tgggtgtcga gcgggacgct gtagcgggtg cccggcgggt
     4741 tgctcgagac cccaaactcg gtgaagtaga tcttcgacga gatcccgcgc ggcacgaggc
     4801 ccgagtgctc accctgccgc gcaaccgcct tgagccgcga cgtgtaggcg atcgtgatgt
     4861 tggcggacga ctgccttgtg gtgagcggat cgccggctcc gcgcgtgtac gggtggtggg
     4921 cgatcccgcc gaccgcgaag cggcgcggct tcgagcagcc gatcgcgctg gcggccgagc
     4981 ccttgagctt gcgacccctc gagtcgacgc agaagagcgc gcggtagaac gtgaccggcg
     5041 cggcgccgcg gctcccgccc gcgatcggcg ccgactcgcc cagcagcacc tggtccctgc
     5101 ggtggccccc cgcgcgcagc ccggcgttgc cggcgtgcac gatcgagcgg tacatcgcgg
     5161 cgcccttctg gatcgtcctc cccttcacgc gcgcggtctg gggggacagc cacgagttga
     5221 ggttcggctc gttccagaac gaccagcgct tgaccgcggg gagcttgccc tcgccggact
     5281 cgtccgtata ccggccgctg tagcggcgcg cgagcgcctc cacgaactgg ccgagcttgc
     5341 ccgggctcgg ccggcaggtt ccggggttgt acttctcgcg ctcggactcg gagcagccct
     5401 ccgcccagcg cggcgcgggc ccgctcgggg tcatcagcac ctgcatcccg cgcgcggccg
     5461 cgccacggac gaggtcgtcg taggtgttcc agcggaccgg gtcgtacgag cgcgggtcgt
     5521 cgagcttcac gcccttgggc ggcttcagcg cggcgttgtc gggcgcgagc ccgcgccagc
     5581 tgatgaccgt gtggatcgtg tccacgccga gcgccgccat ctggtcgagc gcgcgcgcct
     5641 gagccgccgg cccctcgttg tagagcacgt ggtcgtcctg catgatcgat tcctggctcg
     5701 tggacgcggc ggccgtcccg ccgagggcgg gcagcgcgag ggccgcgacg gtggcggcga
     5761 cgagcgcacg cgaccgcgcc ggccgaaggt ttgcgaggga gggcattggc ggttccttcg
     5821 gggcggttac agcggcggga acactagaag aggccggcgg ttgcgaaagt gcactacgcg
     5881 acatggccgc gatctccccc atctgctggc gcgacggcgt tctcgagctg ctcgatcagc
     5941 gttgcctgcc cggcgaggag gcatggctgc gctgctccgc aacttgtcag gttgctgacg
     6001 cgatccgatc gctcgcggtg cgcggggcgc ccgcgatcgg gatcgccgcc gcctacgggt
     6061 tggcgctggc cgcggcgcgc ggcgaggacc tcgccgaggc cgcgcggatg ctcggcgaca
     6121 cgcgaccgac ggccgtgaac ctgcgctggg cgctcaatcg cacgctcgcc gccggccgcg
     6181 agggcgcact ggccgaggcg caccggatct tcgcggagca ggtcgaggcc gaccgccgca
     6241 tgggcgagct cggtgccgag ctgctcgcgg ccggcgaccg cgtgctgacg cactgcaacg
     6301 ccggcgccct cgccacgggc gggcgcggca cggcggtggg cgtcctgcgc tcggcgtggg
     6361 agaccgggcg gctcgcgcac gtgtgggtgg gcgagacgcg gccgctgctc cagggcgcgc
     6421 ggctcacggc gtgggagctc gagcgcgagg ggatccccta ccgcgtggtc gccgactcgg
     6481 gcgccgggtc gctcatggcg cgcgggctgg tgagccgggt gatcgtcggc gcggaccgca
     6541 tcgcgcgcaa cggcgacgtg gccaacaagg tcggcaccta cccgctggcg gtgctcgcgg
     6601 accgccactt gatcccgttc tacgtggcgg cgccggtctc gacgatcgac cacgcgacac
     6661 cgagcggcga cgccatcgag atcgaggagc gcgagcccgc cgaggtggtc gagctgggcg
     6721 gccgccggat cgcgccgcct ggagcggagg cggcgaaccc ggccttcgac gtgaccccgg
     6781 cggagctcgt gaccgcgatc gtcacggagg ccggcgtgct cgagccgccg tacgaggagg
     6841 cgatcgccgg tgtcctcggc tgaccgcggg cggcgcggcg gcgagccacg gccggccacg
     6901 cccggcgagg cccggcgccc ggcgctcgac gccgacgccc tgctcggcgc cgcccggcgg
     6961 ccgctcgtcg tgggcatcgg cggcggaggt gacgtggtcg gcgcgctcgc gaccgccgag
     7021 gcggctcggc tccagcacgg cgctcggccc gtggtcggcg gggtcacctg ggagcggcgg
     7081 ccgatcgacc cggttccggg cccgcgccac ctgaacgaga tcgagggcgg ccgccagctg
     7141 gcgccgggag tgctcgccgc cggcccggac acccgcgtgc gtgagagcgg cgtgctcttc
     7201 ggcgagtcgc ggatggccgg cttcctcggc gaggagaccg tgctggtcga cgcgaacggc
     7261 ggtcccgccg ccgtcgccgc cggcctgacg gcggccgtcg acgccctcgg cgcggacctg
     7321 gtcgtgttcc tcgacgtcgg cggggacgtg ctcgcacacg gcgacgagcc cggcctcgcc
     7381 agccccctgt gcgactcgat cctgctcgcg gcggccggac gcatggcggc ggcgggcgtc
     7441 ccgacgctgg gcggcctctt cggcatcggc tgcgacggcg agctgacgcc gcgcgaggtg
     7501 gatgcgcggc tggccgagat cggcgcggcc gggggcctgg ccggcgcgcg cggactgacc
     7561 gagcccgtcg ccgcgctgct cgagggcgcg atcgagcacg tgccgaccga ggcgagcgcg
     7621 atggccgtgc gcgccttccg cgggcagacc ggcccgaccg cgatccgcgc cgggcgccgc
     7681 acgctcgagc tcacgatggc cgcggccgcg accgtctact tcgacgtgac ggtcgcgatg
     7741 gccaccgcgg cccgcctgcc cgcgctcgtc gacccggcga cgagcctcga cgaggccaac
     7801 gacgccctca acgccgccgg catccgcaca gagctcgact acgagcgcga gaacgcctac
     7861 ccgcgagcgc cgggcgccta gccccccggg aggctcgagc ccgcgaagaa caggaaccag
     7921 atcccgaacg cgagcaggca gagcccgacc gagcagatca tcacgaactc gagcaccgtc
     7981 gtccgccggc cgacgtcgcg ctcgccgcgc atgctgcggt gccaggggag ctgcacccgc
     8041 acgtccgagg tctgcccggt cacccgctcg taggcaccgt tcatccggta gaggaagcgg
     8101 ccgaagagcc acatcgagac cggcaccccg aagagaattg ccaggagcgg ccagagctgc
     8161 ggcgcggtcg tgtccaccat ctgcgagacg ccgtagatcc acagaaacgg gatcccgagc
     8221 cacagaaaga cgctcccgac ggccatcacg acgatcaaca ggagcgccgt cggcttccgc
     8281 agaatcgaca ggctgaggcc acgcgcggca ggctccggcg ccacggagac atcgtaatcg
     8341 gctctcactc aatatcctcc cggcccgcat gaggggatgc acatgaagct cggcgtgccc
     8401 aaccagggtt ccgccggcga atcgcgcgtg gccacggtgc ccgacgtcgt ccgccggcta
     8461 tcgaagaagg gcgtcgaggt cgtggtcgag accggggccg gcgcccgctc gcacgtgccc
     8521 gacgaggagt tcgaggccgc cggcgcgacg atcggctccg ccgagcagac ctggggtgcc
     8581 gacgcggtcg cgttcgtgaa ggcccccggc gccgccgaga tcgagcgcct ccggcgcggc
     8641 gccgtcgtga tgggcttcct caacccgctc accgacaccg agaccgcgag cgcgctcgcg
     8701 agcgccggcg tcacctcgtt cgcaatggag gcgatcccgc ggatcacccg cgcccagtcc
     8761 atggacgcgc tctcgagcca gagcaccgtg tccggctacc gcgccgtgct agtggccgcc
     8821 gaggagctcg accgcttctt cccgatgctc accaccgccg ccggcacgat caagccggcc
     8881 accgtgctgg tgctcggagc cggcgtcgcc ggcctccagg cgatcgcgac cgcccgccgc
     8941 ctcggcgccg tcgtgaccgg ctacgacgtg cgcgcggtgg tgcgcgagca ggtcgagtcg
     9001 ctcggcgcgc gcttcctcga gctcgacctg gagaccgacg ccgagggcgc cggcggctac
     9061 gccaaggccc tcgacgagga ccagcagcgc cgccagcagg aggcgatggc cgaggccgcc
     9121 ggcaagttcg acgtcgtcat caccaccgcc ctcatccccg gccgcccggc gccgctgctg
     9181 ctgatcgcga aggccgtcga gaacatgagg ccgggctcgg tcgtcgtgga cctcgccgcg
     9241 gtcaccggcg gcaactgcga gctgacggag ccgggccaga ccgtcgagcg ccacgacgtg
     9301 aagatcctcg gcccgctcga cctcgcggcc gagatgccca cccacgcgtc gcagctctac
     9361 gcgcgcaaca tccagtcgct ggtcgagctg atggtcggcg aggagggtgc gctcgagctc
     9421 aactgggagg acgagatcat cgccggcgcc tgcatcacgc gcgacggcga gatcgtccac
     9481 gagggcgcca agaaggcagc gggcgcgccc gcctcacagg cataggagaa gccatggaca
     9541 tcatcacgat gatcgccatc ctcgtgctcg ccgcgttcgt cgggttcgag gtcatctcga
     9601 aggtgccgaa cacgctgcac acgccgctca tgtcgggcac caacgcgatc cacgggatcg
     9661 tgctgctcgg cggcctgctc gtgatctcgc gggccgacgg cgtgctcgac aaggccctcc
     9721 tcacgatcgc gatcgccttc ggcacgatca acgtggtggg cggcttcctc gtgaccgacc
     9781 ggatgctcgg catgttcaag ggccgcgaga agaagaagga acctgcggcg gccgatgccg
     9841 cgtccggtga cggcagcgga ggcggctcgt gacgacgctc gcgacctcct tcgtgcagga
     9901 ccgcgacttc atcaacgcgc tctacatcgt cgcgttcgtg ctgttcatca tcggtctgcg
     9961 cgggctcggt gggccgaaga cggcggtgcg cggcaacaag atcgcggccg tcggcatgct
    10021 gatcgcgttc gtcgccaccc tgctgatcga cgacgtgatc gcggactcgg aggacgccat
    10081 cctgatcgcg gtcggcgtga tcgccggcac cgccatcggc gtgcccgcgg cgcgcagcgt
    10141 gaagatgacc gcgatgcccc agatggtcgc gctgttcaac ggcgtgggcg gcggggcggt
    10201 ggcgctgatc gcgtgggcgg agttccgtca cggcggcgcc gacctcgagc tcaaggtgct
    10261 catccccatc ctgatcgccg cgatcatcgg ctcgatctcg ttctggggat cgaacatcgc
    10321 gttcggcaag ctccaggaga tcctccccgg gcggccgatc tcgctgccgg gccagtcgat
    10381 catcaacctg ctgctcgcgc tcgtgaccgt cggcacggcg atcgcgatcg ccgccggcac
    10441 ggactccgag gcgctgttcg tgctcggcat cctggtcgcg ggcgcgctgc tcgggaacct
    10501 gatggtgctg ccgatcggcg gtgccgacat gcccgtcgtg atctcgctgc tgaacgcgtt
    10561 caccggcgtc tcggccgcgc tcgccggcat cgcgctggag aacaccgcgc tgatcgtggc
    10621 cggcatcgtc gtgggcgcgt ccggctcgat cctcacgaac atgatggccg tggcgatgaa
    10681 ccgctcggtg ccggcgatca tcgccggcgg cttcggcggc gtgaccacgg cgcccaccgc
    10741 cgacggtgac gaggaccgca ccgtgcgctc cacctcggcc gacgacgtgg cggttcagct
    10801 cgcctacgcc gaccgcgtgg tggtggtgcc cggctacggc atggcggtcg cgcaggcgca
    10861 gcacaccgtg cgcgagctgg ccgacgagct cgagcagaac cacggcgtgg aggtgagctt
    10921 cgccatccac ccggtcgccg gccggatgcc cgggcacatg aacgtgctgc tcgccgaggc
    10981 cgacgtggag tacgacaagc tcaaggagat ggacgagatc aacccggact tcccgcagac
    11041 ggacgtctcg ctcgtgatcg gcgccaacga cgtgacgaac cccgacgcgc ggacgaacga
    11101 gggctcgccg atctacggca tgccgatcct cgacgtcgac aagtccggcc aggtgatcgt
    11161 gctcaagcgc agcatgaaca cgggcttcgc cggcatcgac aacccgctct tctacgcgga
    11221 gaacaccgcg ctgctgttcg gcgacgcgaa gaagtccgtc cagcaggtgc tcggcgaggt
    11281 gaaggagctc ggctgagggg gccgaccggg tcgctcacgt agcggcctac cccttcccgc
    11341 gggcggagac gtcgtcggtc ctggtccgcg accacgcggt cgagttggtg gagttcgagg
    11401 agtcccggat cgaggacagc ctcgtgcgcg cgcccgacgg cgacgtggtg gcgctcggcg
    11461 gttactgcga gtcgctcggc atctcccgcg agcgcgtgcc ggccgacccg gtgcgcctgc
    11521 tgtcctacgg cgcgaacgcg tcgcccgacg agctggcgcg caagctcgcc ggccacgacc
    11581 cggtggtgcc cgtggcgctc accgagatcg gggacctcga cgtggtcttc tccgcgcacg
    11641 cgtccccccg cggcgggctc ggcgccgcgg tacagcgcag ccccggaacg tggatcgagg
    11701 tggcgatcac ctacctccac cccgacctcc tcccgctgat cgacgcgacc gaggagaact
    11761 accgccgcgt ggcgatgccc tcgcgcgggg tcgagttcta cgtctccagg cacggctgcc
    11821 tggtgctcga cggctcggag gtggcgatgg ccgcggtccg tgcgcgcggc cggcgcttcc
    11881 cggcgatgac gacgctcgag gccgtcgagg ccgtgcgcgg cctgctcgcg ccggacgtgc
    11941 cgctcgaccg gttcgtgcac gagaacgcga cggacgccga gctgcgcgcc cagcggaccg
    12001 aggtgctgcg ccgcggcgct aagcccttcg gcccgtgaag cagacctgca cgacggcgaa
    12061 gtagaactcg cccgactcgc ccagctcgcg ctgctcggcc gcccaggcgc tcgcctcgtc
    12121 ggcgagcccg ctcgcggcga cgaacggctc gaccacgccg atcagcgagc cgccgtaggc
    12181 gtcgagcgtc agctcggcgg tcgcgaacgt gtgggcctcc atgcggacgt cctcgaagcc
    12241 tcccgcgcgc atcagcggag ccagccgccg cggcagcgcc gggtcgatca ggtgcccgtc
    12301 ccacgcctcc agcacgcggc gcatgcgcac gggatcgcgt gagtgccagg acaccgtcgt
    12361 ccagtcgatg tcccacagga ccacgcgccc gccggggcgc agcacccggt ggacctcggc
    12421 cagtgcggcc gccacgtcgg gcacgtactc catgacctgc accgagagca ccgcgtcgaa
    12481 gctcgcgtcg tcgaacggca gcgcggtcgc ctctccctcg ccgaacgagg tgccgggaag
    12541 cgacttgcag cggtcccgcg cgaccgcgag catcgccggg ctctggtcga tgccggtcac
    12601 ggtcccgtcc ggcccgacct cctcggccag ctcggcgacg tagtaaccgg ggccgcagcc
    12661 cacgtcgagg atgcgctcgc cgggcgccgc cgccacggct tcgcgcacca gccggcgcct
    12721 gcggaccacg tcgcgggtcc ggtagacggc gtcgagcctc tgcgcgagat ccgcgctgtc
    12781 gaagtccatc tccgccactc cccgcaatac ttccagacgt ggagcgccac gaggtacttg
    12841 tgataggcgc gggcccggcc gggctcgcga ccgcggccgt gctcgagcgc gcaggcatcc
    12901 cggcaccggt gctcgagaag agcgacaacg tcgccgccag ctggcgccgg cactacgacc
    12961 gcctgcacct gcacacggtc cgctggctct cgaacctgcc gggctaccgc ttctcgcgca
    13021 agcacgggcg ctgggtctcg cgcgacgggg tggtccgcta cctcgagggc tacgtgcgtc
    13081 accacgagct cgacgtgcgc accggcgtgg aggtccagcg gctcgagcgc gacgacgccg
    13141 ccgacgagtg ggtgctcgac acgccgggcg gggagctgcg cgctcgccac gtggtggtgg
    13201 ccacgggcta caaccacacg ccggtgatgc ccgactggcc cggccgcgac ggcttcgagg
    13261 gcacgctgat ccacggccag gactaccgca accccgagcc gtaccggggt caggacgtgc
    13321 tggtggtcgg ctccgggaac accggcgccg agatcgccgt ggacctggtc gagggcggcg
    13381 cggcgcgcgt gcggatcgcg gtgcgcacgc cgccggcggt gatgctgcgc gaggcgaacg
    13441 ggctcccgtc ccagatcacc ggcgtgatga tgcgccggtt gccgctgccg gtggccgacg
    13501 cgctgtcgcg cagctcggcg cgcatgctga tcggcgacct gtcggagtac gggctgccgc
    13561 cggtcgagcg cccgatcaca cgggccgtga aggaggacgt gatcccgata ctcgacgtcg
    13621 gcctggtcga gctcgtgaag gagggccggg tcgaggtggt ggcgggcgtg gtgagcttcg
    13681 agggccgcga cgtggtgctg gccgacggct cacgggttca gcccgacgcg gtgatctcct
    13741 gcgcgggcta cgcgcgcggg ctcgagccgc tcgtcgggca cctcgggctg ctcggccaca
    13801 aggggcggcc gaccgtgcac ggcggcgaga cccacccgaa cgccccgcgc ctgtacttca
    13861 tcggctacac gaatccgatc agcggaatgt tccgcgagtt ccggatcgtc gcgccgaaga
    13921 tcgcgaaggc gatcgcgcgc gaccgcgggg cgccgcggcc cgcggggcgc gagcgcgagc
    13981 ccgcggcggc gtagccggcg gcgcacccgc gcggcggcgc tcagccgcca gggcgcggcc
    14041 ggcgctatta cgcgccgccg agcgcgcggc gcagcaggtc gcgcagctcg cgctggtcct
    14101 tcgcggacag ctccgctatc ggcggcggcg gggaggtcat ccgcgccgtg atcagctcgc
    14161 gcacgcggcg accctcgccg gtcagcacga gcagcttcac gcggcggtcg agcggcgacg
    14221 gctggcgctc caccaggccg cgggtctcga ggcggtccac gatcccggtg acgttcgagt
    14281 tgtcgcacat gagcgcgccc gccagggcgc tcatcggacg cggctcgtcg agcatcttca
    14341 gcgcgaggag ctgctgcggc gccaggtcga gctcctgccc cagctcgatc agccgtggcc
    14401 ggtgatgggc gaagaagagc tgtaggagga ggccccaagc ctcggccgcg ggactgctcc
    14461 tcggactggc ggtctttgag gccgtcgcca tttgcggtaa atcatagtcg aatcatcgga
    14521 tagttgacaa cctcaaagac tttgctagtc tgcccggcca tgacacagcc agagggaatc
    14581 gaggtcgagg gcctcgtcag ggaattcaag aagggtccgc gtgcggtcga cgggatcgac
    14641 ctcgaggtcg cgccgggcga gatctacggc tttctcggcc cgaacggggc cggcaagtcc
    14701 accaccgtcc acatgctcac cacgctgcta ccgcccacgg cgggcaccgc gcgagtgggc
    14761 gggttcgacg tggtcaagca gggcccgcag gtgcgctcca cgatcggcgc cgcgctccag
    14821 gacgcggcgc tcgacccgct catgaccgcg accgagcaca tgaagctcca gaccgccctc
    14881 cacggcctcc ccaaggccga gcgcgatcgc cgcggccgcg acctgatcga gcgggtcggc
    14941 ctgagcgagg ccgccgaccg gcgcgtgaag ggctactccg gcgggatgaa gcgccggctc
    15001 gacctcgcgc tggccctcgt gcacgagccg cgcatcctgt tcctcgacga gccgaccacg
    15061 ggcctcgacc cgcagagccg cgccgcgctg tgggacgagg tggcccggct cgcgaacgag
    15121 gacggcgtga cggtcttcct caccacccag tacctcgagg aggccgacgt actggccaac
    15181 cgcgtgggga tcatcgacaa cggccgcatc gtcgccgagg gcacgcccac cgagctgaag
    15241 gcccagatcg gccgcccgac cgtggaggcg atcccgcgcg atcccgacca gcgcgaggcc
    15301 gcgcaggagg tgctgcgccg gttcggcgag gacttcaacg aggcctcctc acgcggcgcg
    15361 gccgtaaggc tcacccgcgg cgtcgacgag ctggcgtcga tcgtgcgcgc actcgacgac
    15421 gacggcatcg cgctcgcgaa catcgagctg caccacccca cgctcgacga cgtgttcctc
    15481 gccaagaccg gccgctcgct cgagggcgcg gaggaggacg aggtcgagga gcccgtggcc
    15541 gcgtgagcgc ctcgctcgcc atgccgcgcc aggcgttccg cacgccgctc gcgatccagg
    15601 tcgggtacat cgcccgccgc tcggtgatcc ggacgctgcg ccagccggcg atcatcgtcc
    15661 ccggggtcac gttcccgctg ttcctcctcg cggtgaacgc gggcgggctc gacgcggcca
    15721 cgcacctgcc gggcttcccg accgactcct acctaacgtt cgcgctcgcg atcacgttca
    15781 tgcaggcggc gctgttcgcc acgaacgccg gcggcaacga cctcgccgag gacatccgca
    15841 ccggcttcct cagccgtctc tccctcaccc cgctgcgcgg ctcggcgctg ctcgcggggc
    15901 agctcgccgg catcgtcgcg ctcgggctgt tccaggccgt cgtgttcatc tcggtcgggc
    15961 tcgcggcggg cgggaagatc gaggctggcg cgcccggcgt gctcgtgctg atcgcgctgt
    16021 cggtactgac cgggctcgcg ttcggcaccg tcgggatgct gatcggcctg cgcacgggct
    16081 cgggcgaggc ggtccagggc ttcttcccac tgctcttcgt gatgctcttc ctctcctcga
    16141 tgagcctccc gcgcgacctc atcgagcagg actggttccg cacgatcgcg acctacaacc
    16201 ccgtctcgta cctgatcgag ggctttcgca gcctgctgat ctacggctgg gacggcgagg
    16261 cgctggcgct cgggttcggc gtcgccacca cgatcttcgt cgtcgcgctg gccctgtcgg
    16321 cgcgggcgct ccagacgcgg atggtgcgaa cgtgagctac tactcggtcg cacgtgcggt
    16381 cgcctggcgc cacctcaaca acctggtcaa gaacccggcg ctgttcctgc cgccgctcat
    16441 cttcccgctg ttcttcttca ccgcgttcgc gggcggcctg tcggccgtgg gcgatgcgcc
    16501 cggcttcgac taccccgact acaccgcgtt ccagttcgtg ttcgtgatgc tccagaccgc
    16561 ctcgttcggg ggcgtcttca cgggcttctc gatcgccgcc gactgggaga gcggcttcgc
    16621 gcgccggatg atgctcgcgg cgccgcaccg ctcggcgctg atcgccggct acgcggcggc
    16681 ggcgctgctc cgcaccctgc tcgtcggcgg gatggtcttc ctcgtcgccc tggtcaccgg
    16741 gatggagatc gggggcaacg ggatcgagct cggcggcctg ctcggcctgg ccctgatcct
    16801 caacgtcgcg gccacgctct tcggcgcggg cgtctcgctg cgcacccgca ccatgcaggc
    16861 cgcccccatg ctccagacgc ccgtgttcct gttcctattc ctcgccccgg tctacgcgcc
    16921 gcgcgacctg ctcgcggggt ggatccactc ggtggccgac gtgaaccccg ccaccgcgct
    16981 gctcgaggct gggcgcgccc tgatcgcggg gctccccgcc gaggccggcc tcgcctacgc
    17041 ggtggccacc gggctggccg tgctgttcgc cgtgtgggcc gtgcgcggca tgcgcagcgc
    17101 cgagaacgcc gtgtagctgg cgcgggccgc cgcttgacag acgcggcgcg tcggcagagg
    17161 atcgacccgt gcgcgtaccc ctgctcgcga tcgccgtcgc gttcctcgcc gtgccggcct
    17221 cggccggggc cgccacgtac gacgtcacga cgaccgccga caacctcacc tgcaaccccg
    17281 gcaactgctc actgcgcggc gccatcgagg cggccaacgc ggcgtccggg gccgacacga
    17341 tcaaccttcc ggccgggaac tacgtgctgg agctgagcgg cggcgacgac gacaacgcgg
    17401 tgggcgacct cgacgtcaac ggctcgctca ccctcaacgg cgcgggggcg ggcaccacga
    17461 cggtggacgc tggcgacacc gaggatcgcg tcttcgacgt cgggccgaac gggggcgacg
    17521 cgctcgtcgt cgcgctgcgc gacctgacgg tccgcgacgg caacgccgat ttcgagggcg
    17581 gcggggtccg gagctcggcg cagaccaccc ttcagcgcgt cgtcgtgacc ggcagcgcgg
    17641 ccttcagggg cggcggagtg gcacagacga gctccaccac cctgctgcgg atcctcgaca
    17701 gcaccatcac cggcaactcc ggccagcagg gaggcggcgt cgcgcacgac gcgggcggcc
    17761 acagcgacac gggcgccgtc ggcgtgctga tcgagcgctc gacgatctcg gacaaccacg
    17821 cggttcccgg cggggagggc gaccctgacg gcggcggcat cgcggtcaac ctggacggcg
    17881 acgtgcgggt gcgcagcagc acgatcagcg gcaactcggc cgagggcttc caggacgcga
    17941 gcggcggcgg cgcctatgtc aacggcggcc tgctcgaggt caccaacagc acggtcagtg
    18001 gaaactccgt gaccggcggc tacggcggcg ggatcgccaa caccgacggc gacctgacgg
    18061 tcctgaacgc caccatcgcc ggcaactcct ccacggcggg cggggtcact tcgcgtcgca
    18121 tcgcggccgc ggccgtccag aaccccggcg acccgagcca gcccgggggg aacgtcgact
    18181 cgttcggcga ctccaacacc ttcaagaaca cgatcgtcgc gggcggctcg ccgcagaact
    18241 gccgcggtcg cttcaacacg gtcttcacct cgagcggcca caacctcgag gacgcgaaca
    18301 cctgcgggtt cgacgcgacc ggcgacgccg tgaacaccga tccgcagctc ggcccgctcg
    18361 ccgccaacgg cggaccgacc cggacgcgcg cgctgccgaa gagcagcccg gcgatcgaca
    18421 cggccgacaa cgcggcctgc ccggcgaccg accagcgcgg catcgcgcgg gcgcagcccg
    18481 cgggcggcac ctgcgacaag ggcgcctacg agcgtcccgg gctcgacctc tcgatcacga
    18541 agctcgcgtc ggcggccacg gtcaaggtcg gcgaggaggt gcgcttcacg ctcgcggtct
    18601 cgaactccgg ccccgcggac gccggcggcg tgcgagtgac cgacgcgctg ccggacgggc
    18661 tccagttcgt gtcggccgac ccgtcccggg ggagctgctc gggcacgacg accgtgacct
    18721 gcgagctcgg cacgctggct gccgagcagg acgccgacgt ggtgatccgc gccaccgcca
    18781 cgcgcgccgg cgagttcacg aacaccgcgg cggtggcgtc caacgaggac ttccccgagc
    18841 cgaacagggc caacaacagc gcgagcgtga gcgtgcgcgc ggaggacgtg gcccagggct
    18901 gccgcgacac ggtcgcgccg ctcaccgagc tcaagcagtc gcgcgtgcgc gtgcgccgcg
    18961 gcaaggtcag gctgagcggc gtctcgtacg acccggaccc gtgcgccagc ggcgtgaacc
    19021 gcgtgctcgt gtcgctggcg cgagtgaagg gccggcacct ggtcaactgc cgcttcctcc
    19081 gctcgatgac gcgctacctg ctcacccccg cgcccggcat gaactgccag gaccccgtgc
    19141 tgttccgggc caagggcggc agctcccagg gcaccgcgcg gacgagctac agcttcatct
    19201 acttcgtcga tctccccgac ggcatctacc gcgcccaggc gcgcgcgttc gacaacgccg
    19261 gcaacaagga acggccgacg aagggccgca acatccggaa gttcttcgtt cgctgacctc
    19321 gggtagattc ccgtccgacc cacgagaggg agggcgagtg gccgaggagc agagcacacc
    19381 gcagtcggat cttccgcgcg acgacgatcg cacggacaag cagcaggagt ccgcgtccgg
    19441 tggcggcgac tcgcccgagg acaaggccga gcaggatcag ggcgaggaca ccgaggccac
    19501 cgcgggcgct tccggcgagg gccagagcga cgcgatggag gccgcggaga aggccaagga
    19561 ggagcaggaa gaggccaagg agaagatgaa ggagctcgag gaggacccgc ccgagaagct
    19621 cgaggactgg cccgacgaca agaccaagta cgagaccttc ggcggcgcgg agggcaacga
    19681 gtcctacgac gactcggtca ccgcgaagct cgggccgtcg tcactgcgcc accgcgagga
    19741 cggctcggtc gaggtcgagg gcgaggaggt cgacgacccc gaggagtaca agggcgaccc
    19801 gatccccggc ggccccaccg acccgcaggc cgcggagtca cgcgcctacg gcgagcccga
    19861 cctgaccgac gagacctcgc aggacgtgga gggctcgggg gacgggggcg agggctccgg
    19921 ctccggcgag ggcggcgagg aggacacctc cgagggaacc tcagagggca cttcggaggg
    19981 gacgagcgaa ggcacctccg agggcacctc cagcagcagc tccgacggcg aggacgacga
    20041 cggagacgac gagaagagct agctcttctt cgcctccgcc ttctcctcct cggcctcggt
    20101 cagctcgcgc ttcaggacct tccccgtcgc gttgcggggc agctccttga tgaactcgac
    20161 ctcgcggggc accttgtagc ccgcgaggtt cgacttcacg tgcttcttga cctcgtcctc
    20221 gctgaccccg ctgcccttct tcacgacgaa cgccttcagg cgctggcccc actcctcgtc
    20281 gtcgaccccg atcaccgcca cctcggagac gccctgcatg ttcgagatcg tgtcctcgac
    20341 ctcgcgcggg aagacgttct cgccgcctga gacgatcatc tcgtcgtcgc ggcccgagac
    20401 gaacagccgg ccctcgtcgt cgatgtagcc gacgtcgccc gtcgacatga ggccgtcgat
    20461 cacgtccttc gatccgccgc cggtgtagcc ctcgaagagc atgtcgttgg ccacgaagat
    20521 ccggccctcg ccgccgggcg gcacctgatt gccgttctcg tcgtagatcc gcagggtcgt
    20581 gcccatcggc ggcttgccgg cggtgccggg cgccgcgcgc aggtcgtccg gggtcgcgat
    20641 cgtcgcccag gccacctcgg tcgagccgta gaggttgaag aggatgtcgc cgaactcgtt
    20701 catgaactcg atcgcgagat cgcccggcag ggccgatccc gagagcggga tgctccgcag
    20761 cgacgaggtg tcgtacttgc ggcgcacctc ctccggtagg tccatgatcc gctggaccat
    20821 caccggcacc atcgggcagg actggacctg gtgctgggcg atccccgcga gggtgctctc
    20881 cggcttgaag cggcgctgga gcacgatcgt gtggtgcagc gccatgccga gcgtgaagtg
    20941 cgcgaagccc cacgagtgga acagcggtgc cgcgatgagc gccttgtcgc ccgcgtgcag
    21001 cgggatgcgc gagagcatcg agatggccgg gccgatcccc tcggcctgct tgcgcgaggc
    21061 gcccttgggg gtgccggtgg tgcccgaggt gaggatcacc acgcgcccct gctcctccgg
    21121 cgggacgacg tcggcggggt cgccgtcctg gatcaggtcc tcgagcgtcg ggtccggcgg
    21181 gtcctcgccc tcgtaccacg cgacgaaacg cttgcggcgc ttaccggcgt cggcgagcag
    21241 ctcggcgaac tcctcgtcgt agaccaccgc gacgggcttc tcgcgcttga cgacctcggt
    21301 gagctgcggc ccggcgaagg cggtgttcag gaacagggcg tgcgcgccga gcttcgagca
    21361 cgcgaccacc gtctcgacga agtagcggtg gttgcggcac atgatcgcga cgccgtcgcc
    21421 ctccagcact ccggcgtcgg agaggccgct cgcgaggcgg ttcgtgcgct cgtggacctc
    21481 cgcgaaggtg agccggccga ggtcgtcgat gacggcgtcc tggtcggggt agcgggcggc
    21541 gttggcgatg tacccggtcg cgggcgtgta gccccagcgc tggatggcga tcagcgcacg
    21601 cgcgagccgg tcgggtcgct ccgggcgcac gaggccggcg ctccagacgg tgcggaccgt
    21661 ctcgagcgcc tgctcgacgc ccgccgtcac gctgctcacg aggccaccgt cgttgccgct
    21721 cccgtccccc atgtatcgct ccccctcgac ttcgcgcttg agtcgttcga gtgtctcccg
    21781 caggttgtct ccaaccatcg gcgagctggc gcggtcggcg accatcccga gcacgccgcc
    21841 gggcgcctgg tagctgagcc gcagccacac gcgcgtgcgc ccgtcgtcgg tctcgcgcag
    21901 gcgccatcgg ccgcgctggt cgagcccggt aatgctcgtc caggcgaggt cgccgggggc
    21961 gtcgcactcg accacctcga tcaggctccc gacgtgagcg gagcccacgt gcaggcggag
    22021 ctggtagcgc gcgccgagcc cgcgctcggg gccgccggcg tgctcccagc gcgtggcggc
    22081 ggcgagcagg cggtcccagc gcggcaggtc gccgaccgcc tcccagacct cctcgatcgt
    22141 cgcgtcgatg acgacgctct cgtcgaccct catccgatca ccggcagcgt ccgctgctcg
    22201 gccagggagt cgagcgtggc ctgcatcttg gcgagcacgt actcgtcgac ctcgtcgacg
    22261 tcggggtccg caccgaactg ctcgttcagg tcgatcggct cgagcgcgcg gatctggagc
    22321 ttggccggca gcggcacgtg gccgagcatg tcgccgacgt tcacgcccca cggggccgcg
    22381 agcgagatcg gcagcacctt caggcggaac atgcggtcga gccgcagtag ccgcgccagc
    22441 cgctcgccgc gcgttaggaa gagcgcggtc tcctgcccgc cgagcgagac gatcgggacg
    22501 atcggcacgc cgtgctcgag cgccagccgc acgtagccct tgcggccgtt gaagtcgacc
    22561 cgcgcggact cccagctcgg gcggtgcacc tcgtagtcgc cgccggggta gaccaggagc
    22621 gcggcacccg tgtcgagcgc cttcttcgcg ttctgcggtg acgcggcgac ggtcccgtac
    22681 ctgcgcagcg tcgcgagcca cggcatcgac acgaccaggt tgtgggcgag ctggtagaag
    22741 cgccgctcga ctccgaagta cgtgttgaac gcgagcgtga gcacgagcgt gtccggcgtc
    22801 atgttgccgc cggagtggtt gccgaccagc agcaccggtc cctcggcggg gatgttctcg
    22861 aggcccttca cgtcggcgcg gaagtaaaag ctggcgagca gccagaggcc cggcagcgtc
    22921 tcgcggatgt agtcggggtc gcgctcgtcg aggtcggccg tggggatgcg gctctgcacc
    22981 tgctcggcga tggcgcgtcc gagcgcgctc gccaggccgc ccgcggaggc gctgccgttc
    23041 acggatccgt tggtgcgcgc gcgcctcgcg gccgagcgct cgtactcccg tgcggagctc
    23101 atacggggtg tatacccgct tgggcgatca agccgcctgc ctgcgcactc ccggcagcgt
    23161 ctccggccac tccgacggcg gtccgtcggg ctcgcgaatc ttgtgaggct cgccctcggg
    23221 caggtcgtcg aggtactgcc gcacgccgtc cgcgcggagc tgctcgccga cggtgcggat
    23281 cgcggtgcgg atgaccacgg tgaggttcct gcggctcatc aggttcgggc ccatggcctc
    23341 gtggtcctcg gccgtgggct ggatcagcac cacctcggtg ccgctctcgc gcagcttctt
    23401 gctctcgccg ccgaggcggc ggccggacat cccgcgcatg acccccgcca cccgctcgac
    23461 cgggttccac gcgaacggcg ggtggagcga cgacgtcggg ttcaggcaca cgaccaggtc
    23521 gaggtcgagg ccgcgcagga tgtcgaggtt cgaggtggac cacaggcccc cgtcgacgta
    23581 gcggcgcccg ccgatcctca ccgggcggta gaagccgggg atcgcgcacg aggcggcgac
    23641 ggcgtcggcc agctcggccg gcggggcgtc ggcgcgcccg aacggcacgc gccgcccggt
    23701 ggcgtagtcg caggcgacgg cccagaagtt cgggtggtcg ttccagccgc tcggcaccac
    23761 gcggcggatc gtgtccttca gcggctcgtt cgagatcacg ccctgcggga gccagccggc
    23821 caggagcgcg gacgggcggt gcaggtgcgg ggcggtgacg ctccgcgcga tcaggcccca
    23881 cgagccggga ccgacgcgcg gcagcgcgcg ctcgacgcgg aaggtcgccc cggcggcgcg
    23941 gtcggcggtg tcctgcgggc gcccgtccac gcccatcagc ccctcgaacg accggccctc
    24001 cgagtgggcg accatgaacc acggcggcac gcccgagctg agcaggccgc ccatcatcga
    24061 gccggccgag gtgcccacga tgtagtcggc gctgctggga tcccagccgg tctcggtggc
    24121 gatcgcctcg agcccgccgg tgagccatgc gccgcccatc acgccgccgg cgccgagtac
    24181 caatccgatc cgcggtgacc gtgacatcga tcggtgtcag agtctacggg agccgggcga
    24241 cgcggcgccg ctcgtagagg tgcagcgccg gcgcgtccat ccgcgcggac tcccccacgt
    24301 tccagcgcgc gatcgccagg gtgttctcgc cgcgctcgcg caggacgccg gcgggcaggt
    24361 agaagcgctc ctcgcgatcg cgtccggcgc gggcgatctg cacgccgttc aggtacacgt
    24421 tcgcggggaa ggacgccttc ggcaggtcga gcccgaacgc cgcgtagtga cccctcggca
    24481 cgctcagccg gaagcgcgtg cgataccagg cgacctcgcc ggggccggag ctggtctcgc
    24541 gcggcagcga cacgcgctcc caggaggagt cgtcgaagcc gccgcggtgc cagccggcgc
    24601 cctcgccgtg gaggccgctc gtgttggggt agtcctcggg cgcggccccg tggatccgcc
    24661 agtcgatgcc gcccgacggc ttgggcaggc cgggtgccgt cgagtaggtg cccgcggcga
    24721 gcggcgtgct gaactcgccg ccggggccgg ccgggccgag ggtccagccg cacggcggcg
    24781 cgccggggcg gtcgagcgac gcggagatca gcccgcgcgg gttcttcgcc tggatcgcgc
    24841 cgccggcgtc catgttgtgg ccccagctct cgaccagcac ggcgatgacg ttctcgccgc
    24901 cggtctgggc gtcggccggc agcgcgaggc gcgcgggctg cggcacgggc ggtgacgcgc
    24961 cgaggccgcc gaggcccatc gggccgggca cctccgcggc gctcgtgacg gtgccgaggc
    25021 tgcgaccgtt cagccacacg tggtagcggt ggcggccctc caggcagagc tcgccggccg
    25081 ggccgctgta gcggccgcgg taccagatga agccgctgcc gggcacgccg ttgtcgtcgc
    25141 tgccgagcac ggggctcgtg ttgggctgga cctggttgtg ggtggagagc ttctcgaggc
    25201 gcggccaggc ggagtcgtcg aaggcgggtt ggcgttcggg ggtctcggcc ctgcgtcgcc
    25261 aatcgctcag ccgcgggacc ttcacgcgcg cggggccttc caacgtgcgc cgcttcacgc
    25321 tcccccgcgc gatcgtcgtc gtccgtgttc ggcgcgacgt ttccaccgtc gaccccatca
    25381 gtaagagcgg ccccaccacg agccgcgtgc cgctcttcca gacgcgtgcg gcctgcgagc
    25441 gcgacatgat caccagggtc ctcttcccca gccgcagtat gcgcgggtgc ttcgccggct
    25501 tgaagtcgaa ccgccggccc gcgatccaca ccgagtacgg ccgcccgcgg tcgccgaaca
    25561 gcacgaggaa gcgcttgtcc gcatgcgcga cctccgcgct cgtgaggtcg atcctccagc
    25621 cactcaggtt cgcgcgggcg agcaaccagc gcgccgagtg gccgggcagc ggcaccggcg
    25681 gggtcgtcct gccgtcgatc gtgaccggcg tgtcgcgctc gaccccgggg tccgcgttgc
    25741 ggaggaagac gtgcagcgtg ccgtcgtcca cggaacgccg catccggtag acgccctcgg
    25801 gcgcgtccac ccgctcggtc cgcgcgatcg accctcccgc cgcccggatc tggagcccga
    25861 tccggcgcag ctcgtcgtag cgcgtgccga gcgcgcccca ctcgcgcacc ggcgcgccgt
    25921 agtcgtagcc cgtgccggcg ttcgggtaga cctggttgcc ccacgtcgtc ccgccgtagg
    25981 tcatgtagcg gttgatcatc gtcagcccct ggccgagcag cgagatcgtc tgcacgtact
    26041 cgtagccgat catgtgccgg tagcggtcct cggtcgtgtg accgtcgtcg gcgaaggcga
    26101 tcaggccgcc ctggatctcg gccgcgtaga ggggcgagcg ctgcacgccc acctcgtccc
    26161 ggtagtagtc ctcgaacgtg tcgacctggc ggcggaaggt ctcctcgttc cagtcggcgc
    26221 agcaggcgaa gccgtacggg tagttgtcga agccgaacac gtcgacgagg tccaggaagt
    26281 gcccgcgcga gcgctcctgg ccgaggagct ggacgtcgtt gtgggtgatc gggacctcga
    26341 tcccgaggcc cttggcagtc tgcaccaggt cgcgcatgta cgcgcgcgac tgctcggtct
    26401 cctcggcgcc gccgcccggg tgctcgttct cgacctggag cgccaccatc gtgccgcggt
    26461 tggcgccgcc ggcctgccag cgcgcgaggc gcggcagcac cgcggcgaac cagcgcttcc
    26521 agtcggcggt gaactcgggc tcgccggtgc ggtggatgcc aggcttgccg agcgtccacc
    26581 acgggtagcc gccgccgtcg acctcgccct gcacgtacgg accgatccgc gcgatcacgt
    26641 cgaggccggc gtcgcgcgcc tcgcgcatga accgctcgac gtcccagacg ccgtcgaagc
    26701 ggaagcgccc cggcgcgatc tcgtggtcct gccagaagac gtagatgctg atcgtgtcga
    26761 gcccggccgc cttcagcgtg cgcagcaccc ggcgccactg cgcgggccca ccgggcatgc
    26821 ggttcgggtg gacctcgccg gcgttgacga agacggggcg gttctcgatc cgaagggagt
    26881 acttgtccca ggtgaccggc gttgcggcct ggctcggcgc cgcggccagc gcggcggtta
    26941 cggcaacggc ggcgattacg aggaggcggc gcataccgcg cggcaagcta cccgcgcgcg
    27001 gtggctcggg cgccgccggg aatcgcgcag ctttcctgac ttaagggtct atacgacccc
    27061 taagtcagga atgtcgatcg aaaactaggc cggagccgcc tccggctcca ccgcgctcgc
    27121 gtcctcctcg tccgcccccg gtggcggggt gagcgaggcg gccacgatca ccagggcgaa
    27181 cgccaggagc cgcagggtga agccgagcgg gtcgtccggc atcggctcgc cgaagacgat
    27241 cgggccggct gcgatggtca cgaggttcgc ggcggcgctg gtcgcggcga ttacggggac
    27301 cgccccgccc tgctggaggc tccgcgccga gaccgtgagg ccgatcagcg agagcgacgc
    27361 gatcacgaac gcgaggggat ggagcaggac caggacgccg tcgttgtcga gcgagtcgct
    27421 gagcgccttg atcgacacgt cggaggcgcc ccacaggagc cccgcgccca cgcccacgaa
    27481 cggcgcgcgg gctcggctct cgccccagcc gtatacggcg gcggccatcc cggccgcggc
    27541 gagcagcccg gcgtacagcg ccagggtgcc cgcgccgtag tcggagtgcg cctcgtcgcc
    27601 ggtgccgctc atcgtggcgg cgaggaacgc gagcccgagc gcggtgagcc ccacgccgat
    27661 ccactcgcgc cgcgtcacgc gctggccgaa gagccggtcc gccagcaccg tgagcagcac
    27721 gaggccgccg gcgatcacag actggacgag cgagatcggc gcgagcgaca gcgcggccac
    27781 gtggaggccc cacgagccgg tcgcgacgac gatcccgatc gtgtagtcgc gggcgctgaa
    27841 cagcgcgagc gacgagcgca ccgggcggcg cgcgtcgacc ggcggcgcct gccccgcgcc
    27901 gcggtgcttc agcaggaagc cgagcaccga cgtgaacgcg gtggcgagcg cgaacagcag
    27961 tccgacctga accgtcaccg acatcgcgct gacggtagcc agcggggccg cgatggccga
    28021 gccgcgggcg gtgctgacgc gcccggctcc gccgaggacc gcggcggccg cgcccgtgcg
    28081 cggcggcccg ggcccc
//
LOCUS       DASVPL010000223         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_183367, whole genome shotgun sequence.
ACCESSION   DASVPL010000223 DASVPL010000000
VERSION     DASVPL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183367"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VF517_12005"
     CDS             complement(<1..571)
                     /locus_tag="VF517_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053706.1"
                     /translation="MLELFASQSSPAGGAAAAEAVLATVMAIAITLVISVPLAGHVSG
                     RGRLLAWMAEASAKPSRLPGWAALPNDILGTALLVAVFGMYWDVALHIDDGRDAGPLA
                     NPAHYFILLGLFGVFFAGMASIVLARNERTATSVRLPNGWHAPLGGLVIAFFGALALV
                     AFPLDDTWHRLFGQDVTLWGPTHLILIGSA"
     gene            666..1298
                     /locus_tag="VF517_12010"
     CDS             666..1298
                     /locus_tag="VF517_12010"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8053707.1"
                     /translation="MAPPSHRQQLLDGAIHCLRTKGYARTTARDIAAAADANLASIGY
                     HFGSKDALLDEAVIAVSEEWARKMEQAAFAGEGASPLERMAGSWVAMLAAFEHERPLM
                     VAFVEAVARAQWSDELRGRLAASYRGLRDSVGAMVERSFGGEAPAADGAADPRVVASY
                     LIAVFDGFLVQWLLDPEDTPDGETLIANLGAALASAMEAAAAGSATGPRG"
     gene            1308..>2236
                     /locus_tag="VF517_12015"
     CDS             1308..>2236
                     /locus_tag="VF517_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053708.1"
                     /translation="MTTSAGTERVPVPRDRENDYTREAAQQRADFVREKTGAELEHVT
                     SYSFDPSILTGNVEQFVGVAQVPIGVAGPLLVNGEHAQGEFYVPLATAEGTLVASYNR
                     GMRLLHEAGGVTTTIMDDRMQRAPAFLFASARQARDFRDWIDEHFDEIKAAAEATTRT
                     GKLQDIERYSAGRMLYTRFNYTTGDAAGQNLTGKATAAACAWIAANHPEIEHFFLESN
                     FATDKKSSQVNMLNTRGKRVVAEAVIPDELIRRVMRSDTELMFRARQVSNLGGFMSGV
                     NNNGAHSANGITAMLIATGQDAANVAESSAAFV"
BASE COUNT          364 a          790 c          799 g          283 t
ORIGIN      
        1 aggccgagcc gatgaggatc aagtgcgtgg gcccccacag ggtcacgtcc tggccgaaga
       61 gccggtgcca ggtgtcgtcg agcgggaacg cgacgagcgc gagcgctccg aagaacgcga
      121 tgaccaggcc gccgagcggc gcgtgccagc cgttgggtag gcggaccgag gtggccgtcc
      181 gctcgttgcg ggcaagcacg atcgacgcca tgccggcgaa gaagacgccg aacaggccga
      241 gcaggatgaa gtagtgcgcc gggttggcca gcggcccggc gtcgcggccg tcgtcgatgt
      301 gcagggcgac gtcccagtac atgccgaaga cggccacgag cagcgcggtg ccgagtatgt
      361 cgttcggcag tgccgcccag ccgggcaggc gcgagggctt cgccgacgcc tccgccatcc
      421 acgcgagcag gcgcccgcgg cccgacacgt gcccggcgag cggaaccgag atcacgagcg
      481 tgatggcgat ggccatgacc gtggcgagca cggcttccgc cgccgccgct cctcccgccg
      541 gactgctctg gcttgcgaat agctccagca tcccctgcct cccccaggcc gtcgacgcgg
      601 ccctttgctg tacgtatgtt caatatgaac gtccgtccaa caagctaggg tgggcctggt
      661 cacagatggc cccgccaagc caccgccagc agctgctcga cggcgcgatc cactgcctgc
      721 gcacgaaggg gtacgcgcgc accaccgcgc gcgacatcgc cgcggccgcg gacgccaacc
      781 tcgcctcgat cggctaccac ttcggctcca aggacgccct gctcgacgag gccgtgatcg
      841 ccgtctcgga ggagtgggcg cgcaagatgg agcaggcggc gttcgccggc gagggcgcct
      901 ccccgctcga gcgcatggct ggctcgtggg tggcgatgct cgcggccttc gagcacgagc
      961 ggccgctgat ggtggcgttc gtcgaggcgg tcgcccgtgc ccagtggtcg gacgagctgc
     1021 gcgggcgcct ggcggcgagc taccgcggcc tgcgcgacag cgtcggcgcg atggtcgagc
     1081 gcagcttcgg gggcgaggcg cccgcggccg acggcgctgc cgacccgcgc gtggtcgcgt
     1141 cgtacctgat cgccgtgttc gacggcttcc tggtccagtg gctgctcgac cccgaggaca
     1201 ccccggacgg cgagacactg atcgcgaacc tgggcgccgc cctggcgagc gcgatggaag
     1261 ctgccgccgc cggttccgcc accggcccgc gcgggtagag agcgcgcatg acaacgagcg
     1321 ccggcacaga acgcgtcccc gtcccccgcg accgggagaa cgactacacc cgcgaggcag
     1381 cccagcagcg cgccgacttc gtgcgcgaga agacgggggc cgagctcgag cacgtgacga
     1441 gctactcgtt cgacccgtcg atcctgaccg gcaacgtcga gcagttcgtg ggcgtcgcgc
     1501 aggtgccgat cggcgtggcc ggcccgctgc tcgtcaacgg agagcacgcg cagggcgagt
     1561 tctacgtgcc gctggcgacc gcggagggca cgctcgtggc gagctacaac cgcggcatgc
     1621 gcctgctcca cgaggcgggc ggcgtgacca cgacgatcat ggacgaccgg atgcagcgtg
     1681 cgccggcgtt cctgttcgcc agcgcgcgcc aggcgcgtga cttccgcgac tggatcgacg
     1741 agcacttcga cgagatcaag gccgccgccg aggccaccac ccgcaccggc aagctccagg
     1801 acatcgagcg ctacagcgcc gggcgcatgc tctacacgcg cttcaactac acgaccgggg
     1861 acgccgcggg gcagaacctg accggcaagg cgaccgccgc ggcgtgcgcg tggatcgccg
     1921 ccaaccaccc cgagatcgag cacttcttcc tcgagtcgaa cttcgcgacc gacaagaaga
     1981 gctcgcaggt gaacatgctc aacacgcgcg gcaagcgggt ggtggccgag gccgtgatcc
     2041 cggacgagct gatccggcgc gtgatgcggt cggacaccga gctgatgttc cgcgcccgcc
     2101 aggtctccaa cctcggcggc ttcatgtccg gcgtgaacaa caacggcgcg cactccgcga
     2161 acgggatcac ggcgatgtta atcgccacgg gccaggacgc cgcgaacgtc gcggagtcgt
     2221 cggcggcgtt cgtgca
//
LOCUS       DASVPL010000224         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_338976, whole genome shotgun sequence.
ACCESSION   DASVPL010000224 DASVPL010000000
VERSION     DASVPL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338976"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VF517_12020"
     CDS             <1..406
                     /locus_tag="VF517_12020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8053709.1"
                     /translation="EHIGAGLAVNHGDPRITRVGALLRRFSLDELPNLVNVLRGDMAI
                     VGPRPTIQAQVDQYTDRQRRRLEVKPGITGWAQVNGRASLPWHERIELDVWYVDNRSL
                     RLDLRILARTARMLATGHGLYKGETGGWKPTA"
     gene            388..1374
                     /locus_tag="VF517_12025"
     CDS             388..1374
                     /locus_tag="VF517_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8053710.1"
                     /translation="MEADRVAVLLTGAGKRYDIVSAFAKHAFTIAADPSPLAPAQYAA
                     DLRVVPPPFESDEYVPFLLEVVREHGVRAVVPLTDLDIEILGRSELPAFVPPPDVARA
                     TFDKWEAHELLLARGLPSPPTVLPGDEPEAYPVVVKPRRGSGARSIHVAADREQAEFF
                     ARYVDEPVMVQKLLTGPHFSIDLMCDLDGRCLNAIPRSMLESRGGESIKGKVTRDPEL
                     IELGRRLGEELGVRGPCTFQVFNDPDVGLAIYDVNTRFGGAFPAHMYAARPGRTYPEL
                     VVRMAAGETVDPHVGDFEDGMTFTRWFWQIELDDQLRPTGREIVEGGPRAPR"
     gene            complement(1402..>1594)
                     /locus_tag="VF517_12030"
     CDS             complement(1402..>1594)
                     /locus_tag="VF517_12030"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEX8053711.1"
                     /translation="QALDGPEISDEEHEIVLHEEQPVVQKQTVPKERVRLDKDTVVDE
                     QSVSEEVRKERIDVEGDAR"
BASE COUNT          213 a          583 c          570 g          228 t
ORIGIN      
        1 cgagcacatc ggcgccgggc tggcggtgaa ccacggcgac ccgcggatca cgcgcgtcgg
       61 cgcgcttctg cgccggttct cgctcgacga gctgcccaac ctcgtgaacg tcctgcgcgg
      121 cgacatggcg atcgtcgggc cgcggcccac gatccaggcc caggtcgacc agtacaccga
      181 ccgccagcgg cggcggctcg aggtgaagcc ggggatcacc ggctgggcgc aggtgaacgg
      241 ccgtgcctcg ctgccgtggc acgagcggat cgagctggac gtctggtacg tggacaaccg
      301 ctcgctgcgg ctcgacctgc ggatcctcgc gcggaccgcg cggatgctcg cgaccggaca
      361 cggcctctac aagggtgaga cgggcggatg gaagccgacc gcgtagcggt cctcctcacc
      421 ggggccggca agcgctacga catcgtcagc gcgttcgcga agcacgcgtt cacgatcgcc
      481 gccgacccga gcccgctggc gcccgcgcag tacgccgcgg acctgcgggt cgtgccgccg
      541 ccgttcgaga gcgacgagta cgtgccgttc ctcctggagg tggtgcgcga gcacggcgtg
      601 cgcgcggtcg tgccgctcac ggacctcgac atcgagatcc tggggcgcag cgagctgccg
      661 gcgttcgtgc cgccgccgga cgtcgcgcgc gcgacgttcg acaagtggga ggcccacgag
      721 ctgctgctgg cacgcgggct gccgtcgccg cccaccgtgc tgcccggcga cgagccggag
      781 gcctatccgg tggtcgtcaa gccgcgccgg ggctcgggag cgcggtcgat ccacgtcgcc
      841 gcggaccgcg agcaggcgga gttcttcgcg cgctacgtcg acgagccggt gatggtccag
      901 aagctgctca ccggcccgca cttctccatc gacctgatgt gcgacctcga cggccgctgc
      961 ctcaacgcga tcccgcgctc gatgctcgag tcgcgcgggg gcgagtcgat caagggcaag
     1021 gtcacgcgcg accccgagct gatcgagctg ggccgccggc tcggcgagga gctcggcgtg
     1081 cgcgggccgt gcacgttcca ggtcttcaac gacccggacg tggggctcgc gatctacgac
     1141 gtcaacaccc gcttcggcgg cgccttcccc gcgcacatgt acgcggcgcg accgggccgc
     1201 acgtatcccg agctcgtcgt gcggatggcc gcgggtgaga cggtggaccc gcacgtgggc
     1261 gacttcgaag acgggatgac cttcacccgc tggttctggc agatcgagct cgacgaccag
     1321 ctgcgcccga ccggccgcga gatcgtcgag ggcggtccgc gggccccgcg ctagggcgag
     1381 gcccgcggcc acccgcggcg gctagcgcgc gtcgccctcc acgtcgatcc gctccttgcg
     1441 gacctcctcg gagaccgact gctcgtcgac gaccgtgtcc ttgtcgaggc ggacgcgctc
     1501 cttcggcacc gtctgcttct ggaccaccgg ctgctcctcg tggagcacga tctcgtgctc
     1561 ctcgtcgctg atctccggtc cgtccagcgc ctgg
//
LOCUS       DASVPL010000225         6166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_301044, whole genome shotgun sequence.
ACCESSION   DASVPL010000225 DASVPL010000000
VERSION     DASVPL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301044"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF517_12035"
     CDS             complement(<1..369)
                     /locus_tag="VF517_12035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8053712.1"
                     /translation="MHIETGMISPVISYCVVNTNGRELLMRCLDAIDRTHPPGVEREV
                     LVLDNASDDGSAEAVRERGGADVRLIALDRRTGKAENDSTLLREARGDYALLLNEDAE
                     LQEGAAAALLEALERDPGAAV"
     gene            398..628
                     /locus_tag="VF517_12040"
     CDS             398..628
                     /locus_tag="VF517_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053713.1"
                     /translation="MPKAIQIRDVPDDVHRRLKVRAAREGRTLSELLRQELEVLANTP
                     TLAEVLAEIKSRGPVTLTETPAEAVRAGRAER"
     gene            628..1011
                     /locus_tag="VF517_12045"
     CDS             628..1011
                     /locus_tag="VF517_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8053714.1"
                     /translation="MVADASAVVDLLLDRPAGATLERALGRHAVAHAPHLIDPEVLHA
                     LRRLTQRGGLGERRALGALTDFAALPIVRHAHAPLHDRVWDLRERFTAYDASYVVLAE
                     LLEAEFVTTDSALARSARGLVAVAD"
     gene            1021..2145
                     /locus_tag="VF517_12050"
     CDS             1021..2145
                     /locus_tag="VF517_12050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8053715.1"
                     /translation="MRIQLVDPSAFTPPYDHALAAALARAGADVELVTSRFQYGPVPR
                     GDGYRVTEHFYRRTAARGLDARGRMALKLAEHVPDMLRYRRHAEAADVVHWQWLTVQP
                     LDVRLLAPKRPRVLTAHDVIPREPRPGQIAATRRLAERMDAVVVHSEHGAARLRDELG
                     LAPERIHVIAHGAFDYLTRLPEETPLPAELAAVEGPVVLLFGLLRPYKGIDVLLEAFR
                     EVSGAELWIVGMPRMPIEPLRAAAARVGATVRFVPRFVTDPEIPAFFRRADLVVLPYR
                     EIDQSGVLYTALAFGKPMVLSDVGGFGEVASHGAARLVPPGDPAAVAAALNELLADEG
                     ERARLAEAARRAADGPYSWNRIANRTLDLYRELLGGTGPH"
     gene            2205..3113
                     /locus_tag="VF517_12055"
     CDS             2205..3113
                     /locus_tag="VF517_12055"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX8053716.1"
                     /translation="MSDVAVLGPGGVGGFVAAALARARHRVTVVAREETAAVIARDGI
                     AVTSVRLGDMHARPEAATTLDRPVDALVVAVKATGLAAALARIQAEPHLVVPMLNGLD
                     HVEGLRRRFGPRAVAATIRIEATRVAPGRIEQTSPFLVVEMASADPRMRGSIDAFGDL
                     LNRAGIPARVRDSEADVMWAKLVRLCSLALTTTAYDVPLGPIRETPELRAEHEGCVRE
                     AAAVAVADGADVDPRNTLAELDEAHAELDSSMHRDVSAGREPELDAIAGSVLRAGARH
                     GLDCPTIERLAARVAERAGIAPPAPR"
     gene            3123..4241
                     /locus_tag="VF517_12060"
     CDS             3123..4241
                     /locus_tag="VF517_12060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8053717.1"
                     /translation="MLLAILFWASAALIVHTHAGYALVLALLAKLRPRAGRPALAELP
                     RVSLIVSARDEEEVIADKVRNALALDYPRERLEIVVASDGSTDRTAELARTAGADLVL
                     ELAHGGKVHTQDRAVERASGSILVFSDANTTLEPDALRELVGAFADPAVGYACGHVTF
                     DGGAANQEGAYWRYEMAIRGLESRLAGVTGGNGGLYATRRDAYIVVDPIMGHDLTFPF
                     NMVKRGWRPVYEPNARAREKTTPDNVAEGRRKRRMMSHMWPILLRGGLLDPRGYGPLY
                     AFQILSHRVLRYASPFLHLIALGTNVALLSEGAIYQVTFAIQLATIAAALLAAVVPWR
                     PFRLAQYYVLVTGSILGGLWDWLRTGTTAGWEKAEGTR"
     gene            4238..4834
                     /locus_tag="VF517_12065"
     CDS             4238..4834
                     /locus_tag="VF517_12065"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8053718.1"
                     /translation="MNRALDTLVAGLALAIASPLLLGAMVAIRLESSGSPIYRQRRVG
                     KDGAPFEMLKLRTMVSGAEHVGAGLAVNEGDPRITRIGGWLRRFSLDELPNLVNVLRG
                     EMAIVGPRPTIQAQVDQYTPRQRRRLEVKPGITGWAQVNGRAALPWHERIELDVWYVD
                     NRSLLLDLRILVRTAQMLATGHGLYKGDTGGWKPNEHA"
     gene            4810..5802
                     /locus_tag="VF517_12070"
     CDS             4810..5802
                     /locus_tag="VF517_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8053719.1"
                     /translation="MEAERARVAVLLTGVGKRYDIVSAFAQHAFTIAADPSPLAPARY
                     AADLRVAPPRIDDPGYVPFLQDLVAEHDVGAVVPLTDLDIEVLARADLPAFVPDPEVC
                     RATYDKYETHQLLLRLGLPSPPTVLPGEEPDSYPVMVKPRRGSGARSIHPAADREQME
                     FFVRYVDEPVMVQRLMGGHEFSIDILCDLDGRCLNAIPRTMLESRGGESIKGTVIADP
                     ELIELGRAVAEALPARGPCTVQAFRDPEIGLGITDVNTRFGGAFPAPMYAAGPGRTYP
                     ELIVRMAAGERIDPHVGEFDAGTTFTRWYWQIELDADNEPTGRDIVAGGPRPPR"
     gene            complement(5799..>6166)
                     /locus_tag="VF517_12075"
     CDS             complement(5799..>6166)
                     /locus_tag="VF517_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053720.1"
                     /translation="PAPAVSMTRPPKHVAKQALVKLQGSIEPHTQSISVLVEKKNRST
                     WQRFYGRTVAVRADGTFTKKVRFRYEGLYRASFVFGGDGSYAPSRSKVYHVRVPRSGG
                     SGGGGTSGAPPYEPPASTR"
BASE COUNT          797 a         2375 c         2156 g          838 t
ORIGIN      
        1 caccgcggcg ccggggtcgc gctcgagcgc ctccagcaga gccgccgccg cgccctcctg
       61 gagctcggcg tcctcgttca gcagcagcgc gtagtcgccg cgcgcctcgc gcagcagcgt
      121 cgagtcgttc tcggccttgc cggtgcgccg gtcgagcgcg atcagccgga cgtcggcccc
      181 gccgcgctcc cgcaccgcct cggccgatcc gtcgtccgag gcgttgtcga gcaccagcac
      241 ctcccgctcc acgccgggcg ggtgcgtgcg gtcgatcgcg tcgaggcacc gcatgagcag
      301 ctcgcgcccg ttggtgttga cgacgcagta cgagatcacg ggcgagatca tgcccgtctc
      361 gatatgcatg catgatgcat gctacggtgc atagtgcatg cccaaggcca tacagattcg
      421 cgacgtgccc gatgacgtgc atcgacggct caaggtgcgc gccgcgcgcg agggccggac
      481 cctctccgag ctcctgcgcc aggagctcga ggtgctcgcg aatacgccga ccctcgccga
      541 ggtgctggcc gagatcaagt cgcgcgggcc ggtcactctc acggagaccc cggccgaggc
      601 cgtccgcgcg ggccgggccg agcgctgatc gtcgccgacg cgtcagcggt cgtcgaccta
      661 ctgctcgatc ggcccgccgg cgccaccctg gagcgcgccc tgggccgtca cgccgtggcc
      721 cacgccccgc acctgatcga ccccgaggtg ctccacgcgc tgcgccggtt gactcagcgc
      781 ggcgggctcg gcgagcgccg cgccctcggg gcactcaccg acttcgcggc gcttccgatc
      841 gtccggcacg cgcacgcgcc tctgcacgat cgcgtgtggg acctccggga gcgcttcacg
      901 gcctacgacg cctcatatgt ggtgctcgcg gaactcctcg aggccgagtt cgtaaccacg
      961 gatagcgcgc tggcacggag cgcccgcggc ttggtcgcgg tcgccgacta gctgcccgcc
     1021 gtgcggatcc agctagtcga cccctccgcc ttcaccccgc cgtacgacca cgcgctggcc
     1081 gccgcgctcg cccgcgcggg tgccgacgtg gagctcgtca cgagccgctt ccagtacggc
     1141 ccggtgccgc gcggggacgg ctaccgcgtg accgagcact tctaccgccg caccgccgcg
     1201 cgcgggctcg acgcgcgcgg gcgcatggcg ctgaagctcg ccgagcacgt gccggacatg
     1261 ctccgctacc gccgccacgc cgaggcggcg gacgtggtcc actggcagtg gctcacggtg
     1321 cagccgctcg acgtgcgcct gctcgctccc aagcgcccgc gcgtgctcac cgcgcacgac
     1381 gtgatcccgc gcgagccgcg gccgggtcag attgccgcca cgcgccgtct ggccgagcgg
     1441 atggacgcgg tggtggtgca ctccgagcac ggcgcggcgc gcctgcgcga cgagctgggc
     1501 ctcgcgccgg agcggatcca cgtgatcgcg cacggcgcgt tcgactacct gacccggctc
     1561 cccgaggaga cgccgctgcc ggccgagctg gccgcggtcg agggaccggt ggtgctgctg
     1621 ttcggcctgc tgcgtccgta caagggaatc gacgttctgc tcgaggcgtt ccgcgaggtg
     1681 agcggggccg agctctggat cgtcgggatg ccgcggatgc cgatcgagcc gctgcgcgcg
     1741 gccgccgcgc gcgtgggtgc caccgtgcgg ttcgtcccgc gcttcgtgac cgatcccgag
     1801 atccccgcgt tcttccgccg cgccgacctg gtcgtgctgc cctaccgcga gatcgaccag
     1861 tccggcgtgc tctacacggc gctggcgttc ggcaagccga tggtgctgag cgacgtgggc
     1921 gggttcggcg aggtggcttc gcacggcgcc gcgcgactcg tgccgccggg cgatcccgcc
     1981 gcagtggcgg cggcgctgaa cgagctgctc gccgacgagg gcgagcgcgc ccggctcgcc
     2041 gaggcggcgc gccgcgcggc ggacggcccc tactcgtgga accgcatcgc gaaccgcacc
     2101 ctcgatctct accgtgagct cctcggaggt actgggccac attgaccccg catagcgggg
     2161 ctaacttggc ccagttcggc tgaccggctc gtagcctcac ccccgtgagc gacgtcgccg
     2221 tcctcgggcc cggaggcgtg ggcggcttcg tggccgccgc gctcgcccgc gcccgccacc
     2281 gcgtcaccgt cgtggcgcgc gaggagaccg ccgccgtcat cgcgcgggac gggatcgcgg
     2341 tcacgagcgt gcggctcggg gacatgcacg cgcgccccga ggccgcaacg acgctcgacc
     2401 ggccagtcga cgcgctggtc gtcgccgtca aggccaccgg gctcgccgcc gcgctcgcgc
     2461 gcatccaggc cgagccgcac ctcgtggtac cgatgctcaa cgggctcgac cacgtggagg
     2521 ggctgcgccg gcgcttcggc ccgcgcgccg tggcggcgac gataaggatc gaggcgacgc
     2581 gtgtggcgcc cgggcggatc gagcagacga gcccgttcct cgtggtcgag atggcctcgg
     2641 ccgacccccg gatgcgcggc tcgatcgacg cgttcggcga cctgctcaac agggccggga
     2701 tccccgcccg cgtgcgcgac tcggaggccg acgtgatgtg ggccaagctc gtccgcctct
     2761 gttcgctcgc actcaccacg accgcctacg acgtcccgct cggcccgatc cgcgagaccc
     2821 ccgagctgcg cgccgagcac gagggctgcg tccgcgaggc cgccgcggtg gcggtcgccg
     2881 acggcgccga cgtcgacccg cgcaacaccc tcgccgagct cgacgaggcc cacgccgagc
     2941 tcgacagctc catgcaccgc gacgtgtcgg ccggccgcga gcccgagctc gacgccatcg
     3001 ccggctcggt cctgcgcgcc ggggcccggc acggcctcga ctgcccgacc atcgagcgcc
     3061 tcgccgcccg cgtggcggag cgcgccggga tcgcgcctcc ggccccgcga taatcacccg
     3121 ccgtgctcct ggccatcctc ttctgggcgt ccgccgccct gatcgtgcac acgcacgccg
     3181 gctacgccct cgtgctcgcg ctgctcgcga agctgaggcc gcgcgccggg cgccccgccc
     3241 tcgccgagct cccgcgcgtc tcgctgatcg tctccgcccg cgacgaggag gaggtgatcg
     3301 ccgacaaggt ccgcaacgcg ctggcgctcg actatccgcg cgagcgactc gagatcgtcg
     3361 tcgcctccga cggctcgacc gaccgcaccg ccgagctggc gcgcacggcc ggcgcggacc
     3421 tggtcctcga gctcgcgcac ggcggcaagg tgcacacgca ggaccgcgcg gtcgagcgcg
     3481 cgagcggctc gatcctcgtc ttctccgacg ccaacacgac gctcgagccc gacgcgctgc
     3541 gcgagctggt cggcgcgttc gccgaccccg cggtgggcta cgcctgcggc cacgtgacct
     3601 tcgacggcgg cgccgccaac caggagggcg cctactggcg ctacgagatg gcgatccgcg
     3661 ggctcgagtc gcggctcgcg ggcgtgaccg gcggcaacgg gggcctatat gccacacgca
     3721 gggatgccta catcgtggtc gacccgatca tgggccacga cctcaccttc ccgttcaaca
     3781 tggtcaagcg cggctggcgt ccggtctacg agccgaacgc ccgcgcccgc gagaagacga
     3841 ccccggacaa cgtcgccgag ggccgccgca agcgccgcat gatgagccac atgtggccga
     3901 tcctgctgcg cggcggactg ctcgacccgc gcgggtacgg ccccctctac gcgttccaga
     3961 tcctctccca ccgggtgctg cgctacgcct cgccgttcct gcacctgatc gcgctcggca
     4021 ccaacgtcgc gctgctgagc gagggcgcga tctaccaggt caccttcgcg atccagctcg
     4081 ccacgatcgc ggctgcgctg ctcgcggcgg tcgtgccgtg gcggccgttc cggctcgccc
     4141 agtactacgt gctcgtcacg ggctcgatcc tcggcgggct ctgggactgg ctgcgcaccg
     4201 gcaccaccgc cggctgggag aaggccgagg gcacgcgctg aaccgcgcgc tcgacaccct
     4261 ggtcgcgggg ctcgccctcg cgatcgcgag cccgctgctg ctcggcgcga tggtcgcgat
     4321 ccggctcgag tcgagcggct cgccgatcta ccgccagcgc cgcgtcggca aggacggcgc
     4381 accgttcgag atgctgaagc tgcgcacgat ggtgtccggt gccgagcacg tcggcgccgg
     4441 cctggccgtg aacgagggcg acccgcggat aacgcgcatc ggcggctggc tgcgccgctt
     4501 ctcgctcgac gagcttccga atctcgtgaa cgtgctgcgc ggcgagatgg cgatcgtcgg
     4561 cccgcgcccc acgatccagg cgcaggtcga ccagtacacc ccgcgccagc gccgccgcct
     4621 cgaggtgaag cccggcatca cggggtgggc gcaggtcaac ggccgcgccg cgctgccgtg
     4681 gcacgagcgc atcgagctcg acgtctggta cgtggacaac cgctcgctgc tgctcgacct
     4741 ccgcatcctc gtcaggaccg cacagatgct cgcgaccggg cacggcctct acaagggaga
     4801 cacgggcgga tggaagccga acgagcacgc gtagccgtcc tgctgacggg cgtcggcaag
     4861 cgctacgaca tcgtcagcgc gttcgcgcag cacgcgttca cgatcgcggc cgacccgagt
     4921 ccgctcgcac ccgcgcgcta cgccgccgac ctgcgcgtgg ccccgccgcg catcgacgat
     4981 cccggctacg tcccgttcct ccaggacctg gtggccgagc acgacgtggg cgcggtcgtg
     5041 ccgctcaccg acctcgacat cgaggtgctc gcgcgcgccg acctcccggc cttcgtcccc
     5101 gaccccgagg tctgccgcgc gacctacgac aagtacgaga cccatcagct cctgctgcgc
     5161 ctcgggctgc cgagcccgcc gaccgtcctg cccggcgagg agcccgactc ctaccccgtg
     5221 atggtcaagc cgcgccgcgg ctcgggcgcc cgctcgatcc accccgccgc cgaccgcgag
     5281 cagatggagt tcttcgtgcg ctacgtcgac gagccggtga tggtccagcg cctgatgggt
     5341 ggccacgagt tctcgatcga catcctgtgc gacctcgacg gccgctgcct gaacgcgatc
     5401 ccgcgcacga tgctcgagtc gcgcggcggc gagtcgatca agggcacggt tatcgcggac
     5461 cccgagctga tcgagctcgg ccgcgccgtg gcggaggcgc tgcccgcgcg cgggccgtgc
     5521 acggtgcagg cgttccgcga ccccgagatc ggcctcggca tcaccgacgt gaacacgcgc
     5581 ttcggcggcg cctttcccgc accgatgtac gcggccgggc ccggccggac ctacccggag
     5641 ctgatcgtgc gcatggcggc gggcgagcgc atcgacccgc acgtgggcga gttcgacgcg
     5701 ggcacgacgt tcacgcgctg gtactggcag atcgagctcg acgccgacaa cgagccgacg
     5761 ggccgcgaca tcgtcgccgg ggggccgcgc ccgccgcgct agcgcgtgct ggccggcggc
     5821 tcgtacggcg gcgcgccgct cgtgccgccg ccgcccgacc cgcccgagcg cggcacgcgc
     5881 acgtggtaga ccttcgagcg cgacggcgcg tacgagccgt cgccgccgaa cacgaacgag
     5941 gcccggtaga gcccctcgta gcggaagcgg accttcttgg tgaaggtgcc gtcggcgcgc
     6001 acggcgaccg tgcgcccgta gaagcgctgc cacgtgctgc ggttcttctt ctcgacgaga
     6061 accgagatcg actgcgtgtg cggttcgatc gagccctgga gtttcacgag cgcctgcttg
     6121 gccacgtgct tcggcggccg cgtcatcgag acggctggcg ctgggc
//
LOCUS       DASVPL010000226         3448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_301297, whole genome shotgun sequence.
ACCESSION   DASVPL010000226 DASVPL010000000
VERSION     DASVPL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301297"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VF517_12080"
     CDS             <1..587
                     /locus_tag="VF517_12080"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX8053721.1"
                     /translation="DVDRLQDPVGRPTSLVALLGPVRRGTLGVANALGTGVADDKLVH
                     AYVEEMIGFYLGEEAVLRSVPTYDLAEPSTLAYVLGRLGELVVKPRSDYGGRGVVIGP
                     HASDDDLAQVARMIAARPDRFIAQETIPLSRHPTVCDGRLEPRHVDLRAFVLSSGDDS
                     HVVPGGLTRVALSPGALVVNSSQNGGGKDTWVAR"
     gene            584..1363
                     /locus_tag="VF517_12085"
     CDS             584..1363
                     /locus_tag="VF517_12085"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX8053722.1"
                     /translation="MTGAPLIGLTTSEVRGAKEFKPTPEGDPPRKDLALGFKYFEAVE
                     RAGGIPVILPPLGPASVDPLLANLSGICMAGGPDIDPRTYGGPDHPELGPTEPELDRF
                     EMAVTWAARSRCIPILAICRGAQMLNVALGGSLHMHLPDDAGDRVEHRRAGSEGPAAQ
                     HEVRVERDSILGASLGVETATVNSYHHQAVRKLGRGLRAVAHAEDGVIEGIELAGPDF
                     VVGVQWHAEAMDGAAHGALFEAFVAAAARRAVAAGEGRLAA"
     gene            1360..2514
                     /locus_tag="VF517_12090"
     CDS             1360..2514
                     /locus_tag="VF517_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX8053723.1"
                     /translation="MTTEMRIVPSTRPSWADWQPRSTPYTVGIEEEAMLLDPVGWKLS
                     QTCMEVLEELPPDLAAHATAETHGSALELATGVHQTIGGAVEELCRLRSGLAEALELR
                     GLAVASAGTHPFAVWSDTVVSPGERYQFLYGSLRELARREPTHALHVHVGVPSPDVAM
                     RAANRLRAHLPLLLALSGSSPFWQGRDTGLASARTPLFQAFPRVGIPRAFRDYDDWVE
                     MVEMLVRCGAFPDPSFLWWDVRLQPRFGTVEVRIMDAQARVEDTAALAALVQSLVRLE
                     CEEGHAPEALLSLPEAIEENRFLAARDGMAASFLDPELEMSVPARTIAYELLEACAPH
                     AHDLGCHAELQDVRRLVQTPGAAWQRYTASSRGLGGLLADLADAYVDYRD"
     gene            complement(2511..3368)
                     /locus_tag="VF517_12095"
     CDS             complement(2511..3368)
                     /locus_tag="VF517_12095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8053724.1"
                     /translation="MRLSVVIPTHDRAPVLARCLDALEPQGADEVVVVDDGSSDETPA
                     VLAERPWAKAVRREQAGGRSAAKNTGMRAATGDLVLFLDDDAIAQPGLVERHRDHHRA
                     HPEAHEALLGHVTWSPEVEVTPHMHWLENGGPMFAYPEIADPEDVTWRMLYTVNVSLK
                     RDFLEPFDEELPIYEDTELGFRLSKRGLRLRYDPHALAHHLRTETPERTERRMEEVGA
                     AAAVLYRKHPELREPPPPIRRVGRLKALGAGAASRLGYRGLDRRLDEHRAARAFARGF
                     AAAERGRGR"
BASE COUNT          419 a         1256 c         1322 g          451 t
ORIGIN      
        1 ccgacgtcga ccggctccag gacccggtcg ggcgcccgac cagcctcgtc gccctgctcg
       61 gcccggtccg caggggcacg ctcggggtcg cgaacgcgct gggcacgggc gttgccgacg
      121 acaagctggt gcacgcctac gtggaggaga tgatcggctt ctatctcggc gaggaggcgg
      181 tgttgcgctc ggtcccgacc tacgacctgg ccgagccctc cacgctcgcc tacgtgctgg
      241 gccggctggg cgagctggtc gtcaagccgc gctcggacta cggcggccgc ggcgtggtga
      301 tcggccccca cgcgagcgac gacgacctcg cccaggtggc gcgcatgatc gccgcgcgcc
      361 ccgaccgatt catcgcccag gagacgatcc cgctctcccg ccacccgacc gtctgcgacg
      421 gccggctcga accgcgccac gtggacctgc gcgccttcgt cctgagctcc ggcgatgatt
      481 cccacgtcgt gcccggcgga ctcacgcggg tcgcgctgtc gccgggcgcg ctcgtggtca
      541 acagctcgca gaacggaggg gggaaggaca cttgggtggc gaggtgacag gcgcgccgct
      601 gatcggcctg acgacctccg aggtccgcgg ggcaaaggag ttcaagccca cgcccgaggg
      661 cgacccgccg cgcaaggacc tcgcgctcgg cttcaagtac ttcgaggccg tcgagcgcgc
      721 cggcggaatc ccggtgatcc tcccgccgct cggcccggcg tcggtcgacc cgctgctcgc
      781 gaacctgtcg gggatctgca tggcgggcgg ccccgacatc gacccgcgca cctacggcgg
      841 ccccgaccac cccgagctcg ggccgaccga gccggagctc gaccgcttcg agatggccgt
      901 gacctgggcc gcgcggtcgc gctgcatacc gatactcgcg atctgccgcg gggcgcagat
      961 gctgaacgtc gcgctcggcg gcagcctgca catgcacctg cccgacgacg ccggcgaccg
     1021 cgtcgagcac cgccgcgccg gctccgaggg gccggcagcg cagcacgagg tccgcgtgga
     1081 gcgcgacagc atcctcggcg cgagcctcgg cgtggagacc gcgacggtca actcctacca
     1141 ccaccaggcg gtgcgcaagc tcgggcgcgg cctgcgcgcg gtggcgcacg ccgaggacgg
     1201 cgtgatcgag gggatcgagc tggccgggcc cgacttcgtg gtaggcgtgc agtggcatgc
     1261 cgaggctatg gacggggcgg cgcacggcgc gctgttcgag gcgttcgtgg cggcggccgc
     1321 ccggcgcgcg gtggcggccg gcgaggggcg gctggcggcg tgaccacgga gatgcgcatc
     1381 gtgccctcga cccgcccgag ctgggccgac tggcagccgc gctcgacgcc gtacacggtc
     1441 gggatcgagg aggaggcgat gctgctcgac ccggtcggct ggaagctttc gcagacgtgc
     1501 atggaggtgc tcgaggagct tcctcccgac ctcgccgcgc acgccaccgc cgagacgcac
     1561 ggctcggcgc tcgagctggc gaccggggtg catcagacga tcggcggcgc ggtcgaggaa
     1621 ctgtgccggc tgcgcagcgg gctggcggag gcgctcgagc tgcgcgggct ggccgtggcg
     1681 agcgcgggca cgcacccgtt cgcggtgtgg agcgacaccg tcgtgtcgcc gggcgagcgc
     1741 taccagttcc tctacgggtc gctgcgcgag ctggcgcggc gggagccgac gcacgcgctc
     1801 cacgtgcacg tcggggtgcc gtcgccggac gtcgccatgc gcgccgcgaa ccggctgcgc
     1861 gcgcacctcc cgctgctgct ggcgctgtcg ggcagctcgc cgttctggca gggccgcgac
     1921 accggactcg cgtccgcgcg cacgccgctg ttccaggcgt tcccgcgggt cgggatcccg
     1981 cgcgcgttcc gcgactacga cgactgggtc gagatggtcg agatgctcgt gcgctgcggc
     2041 gcgttcccgg acccgtcgtt cctgtggtgg gacgtgcgcc tccagccgcg cttcggcacg
     2101 gtcgaggtgc ggatcatgga cgcccaggcg cgggtggagg acacggccgc gctcgccgcg
     2161 ctggtgcagt cgctggtccg gctcgagtgc gaggagggcc atgcgccgga ggcgctgctg
     2221 agcctccccg aggcgatcga ggagaaccgc ttcctggcgg cccgcgacgg catggctgcg
     2281 tcgttcctcg accccgagct ggagatgtcg gtgccggcgc ggacgatcgc gtacgagctg
     2341 ctcgaggcgt gcgcgccgca cgcgcacgac ctcggctgcc acgcggagct ccaggacgtg
     2401 cggcggctgg tccagacgcc gggcgccgcc tggcagcgct acacggcgtc ctcacgcggg
     2461 ctcggcggac tgctggcgga cctcgccgac gcctacgtgg actaccgcga ctagcggccg
     2521 cggccacgct cggccgcggc gaacccgcgc gcgaacgccc gcgccgcgcg gtgctcgtcg
     2581 aggcgacggt cgaggccgcg gtagccgagc cggctcgccg cgcccgcgcc gagcgccttg
     2641 agccggccga cccgccggat cggcggcggc ggctcgcgca gctccgggtg cttgcggtag
     2701 aggaccgcag ccgcggcgcc gacctcctcc atccgccgct cggtgcgttc cggcgtctcg
     2761 gtgcgcaggt ggtgcgccag cgcgtgaggg tcgtagcgca gccgtagccc gcgcttcgag
     2821 agccggaagc ccagctcggt gtcctcgtag atcggcagct cctcgtcgaa cggctccagg
     2881 aagtcgcgct tcagcgagac gttgaccgtg tagagcatcc gccaggtcac gtcctcgggg
     2941 tcggcgatct cggggtaggc gaacatcggc ccgccgttct cgagccagtg catgtgcggt
     3001 gtgacctcga cctcgggcga ccaggtcacg tggccgagca gcgcctcgtg cgcctcgggg
     3061 tgagcgcggt ggtggtcgcg gtggcgctcg accaggccgg gctgggcgat cgcgtcgtcg
     3121 tcgaggaaca ggaccaggtc gccggtcgct gcacgcatgc cggtgttctt ggcggccgag
     3181 cgcccgccgg cctgctcgcg gcgcaccgcc ttcgcccagg gccgctcggc gagcaccgcg
     3241 ggcgtctcgt cggaggagcc gtcgtccacc accacgacct cgtcggcccc ctggggctcg
     3301 agggcgtcga ggcagcgggc gagcacgggc gcgcggtcgt gcgtcgggat gaccaccgag
     3361 agcctcacgg gaccggcgcg gccgcggtcg agggcggcac cgcgccctcg gggtcgggct
     3421 cggcctcggc gcgccgcagg ccgcgcag
//
LOCUS       DASVPL010000227        24727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_339549, whole genome shotgun sequence.
ACCESSION   DASVPL010000227 DASVPL010000000
VERSION     DASVPL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24727
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339549"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VF517_12100"
     CDS             <1..825
                     /locus_tag="VF517_12100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053725.1"
                     /translation="LVLVTGAAFGIVWGLVRGNSAGWGSAEVLLTLVSGVALVGAFAI
                     WESRAAAPMLPLRLFRSRAFSAGNVAIFFHWGSALGAVFFMAQFLQVVLGYGPLKSGL
                     GLMPWGVTTVVVAQAVGRRIGRYGERPFIAAGLTLNAVGLIGIALTAGPSVAYWPIAV
                     GLLLSGSGIAMSIPAAQSAVMTSVAPPDMGKASGAFSTMRQLGGAFGVAVLVAIFAGA
                     GSYDSPQAFSDGFVAAAVACAGLSVLGVMAALALPRHAASTRESLDAMPALQAGRT"
     gene            complement(843..1430)
                     /locus_tag="VF517_12105"
     CDS             complement(843..1430)
                     /locus_tag="VF517_12105"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8053726.1"
                     /translation="MEASRRPGQRAGITRELVIAAGRELLIEHGAAGLTMRAVARHLA
                     VAPGALYSHVRTKAELIDEVLDDLLSQVDAPDPLGTDWRGGLYGLMTSTHEVLLEHPG
                     LVPLYVARRGARGPNAQRLGEIMLALLATQGITGTPAREALRALLVYTIGSAAFTAQT
                     PIDPELDDAQLPSPAELETNFENGLRWLLAGIASA"
     gene            1500..2192
                     /locus_tag="VF517_12110"
     CDS             1500..2192
                     /locus_tag="VF517_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8053727.1"
                     /translation="MADIALFPSVLGVRAGVLDAQDRLSRAGHEVTVIDQYDGRSFDD
                     YEEAGAFAESLGYAGLMAGAFAAVERLPDGFIAVGFSNGGGMAEHVALHRDVAGVALL
                     AGVLPLDMLGDRPWPAGVPVQIHYTRDDPFRREDWLESLTGSVRASGASVELYDYAGA
                     GHLFTDPSLPAEYDAAAAEQLWRRVLAFCAACDETRRSASDRLELGVRPLQHRAQYVT
                     GGGEIAHGQVAP"
     gene            complement(2097..3980)
                     /locus_tag="VF517_12115"
     CDS             complement(2097..3980)
                     /locus_tag="VF517_12115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053728.1"
                     /translation="MATAAAPPTLLATRRGKLTLALLAAVAFLDFVDASIVNIALPSI
                     RRDLHFSEQGLQWVTSGYLLTYGGFMLLGGRAADLLGRRRVLVAGVVLIGGASLVGGF
                     AGSSGLLVAARLAQGIGAAFSLPAALSILTTTFQEGSDRNTALGIWGAVAGLASAAGV
                     LLGGFLTEGPGWRWVMFVNPIAAAVVLVGVFRLIDGERPEPQRRSFDVAGAILATAGM
                     LLLVFTLVEAPDQGWGDGRTILGLAGALALLAGFAINEHRAKKPLLPLSIFRVKGLAA
                     ADATQLIASAGVLAMFFFLTLYMQNVLGYSPIQAGASYLPLTFGIGLAAGVSSQLLER
                     VGTRPVSIAGSLIAAGGVLWLSRIPVDGSYVSDLLPGMFIMSLGLGAVFVAVTTAANA
                     GVPADRAGLAAALVNASTQVGGALGLAIFSAIATTRINDLLESHAQPATALTSGFQRA
                     LLACSVFLLAAAVIATRITNTRGETTTEGSPMPAPPTFGAPVIGQTEKALSAILERQL
                     SGTDLNQPAWIALSVSVAAGGPVDRDELTQRVAGALKVSPAEARAHLTTLADARLLES
                     GGSGSLVEVSEAGRQLHGRVRAAITEVTDRLWGDLPVGDLATAGHVLGTVLERANAEL
                     EPV"
     gene            4075..4551
                     /locus_tag="VF517_12120"
     CDS             4075..4551
                     /locus_tag="VF517_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8053729.1"
                     /translation="METPPRPEPPVAEFAGQLLFRLWRASHTRVADALATVGLTPSSF
                     ALLNVIGAREGAIQQQLGAAMGIDPSTMVALIDELEREGLAQRRPSPTDRRARRIVIT
                     AKGRRRRERARKLVADTEDEVLHGLTAAERDELLALLRRALEGAPPQTPWSSAEGD"
     gene            complement(4548..5096)
                     /locus_tag="VF517_12125"
     CDS             complement(4548..5096)
                     /locus_tag="VF517_12125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX8053730.1"
                     /translation="MPELLLLCGSLRQGSTNEAALRTVRDVLPDGWTAVTYDGMAALP
                     HFNPDDDHDPLPAEVVQLRDRLRAADAVLISTPEYAGALPGSFKNLLDWTIGGDEIYE
                     APVAWVNIAGPAAPSGGADAHDSLRKVLSYAHADLVEAACARVPITRADVAPSGLVED
                     AGIRGRLREIVDAIDAHLTARG"
     gene            complement(5096..5908)
                     /locus_tag="VF517_12130"
     CDS             complement(5096..5908)
                     /locus_tag="VF517_12130"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8053731.1"
                     /translation="MQRTAERPAVGPLLREWRQRRRISQLDLALDAGVSARHVSFVET
                     GRSRPSAEMVMQLSEQLDVPLRDRNALLLAAGYAPAYGQRDLDAAEMAPVRAAIDRVL
                     RGHEPYPALVVDRHWGMVAANATIPLLTAGAAGHLLEPPVNVLRLSLHPDGLAPRIAN
                     LGEWRAHLLDRLGRQAVASGDPALFALHEELAEYPSGDRAPVPDLDAAEIAVPLQLRV
                     GDATLAFISTATSFGTAIDVTVSELSIEAFFPADEATAEALRAAQPSSERQI"
     gene            5996..7414
                     /locus_tag="VF517_12135"
     CDS             5996..7414
                     /locus_tag="VF517_12135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053732.1"
                     /translation="MTPPTRTNRTHWAALPVLLAGSFVVVLDFFIVNVALPSIAADLD
                     AGASSLEWVVAAYGLTFAAFLITAGRLGDELGRRRVFVVGLALFTLASTACGLAPSPT
                     TLVLARALQGVAGAIVMPQVLSIIGVTYRDGDYVKALSAYGVALGLSAVGGQVIGGAL
                     VQTDVAGLGWRACFLINVPICLAAIALTPALVRECRAPSRARIDLLGAATLALALVAV
                     LLPLIEGRQHGWPAWTWLSLAIAPLLLAGFVARQARLARRGGSPLVDLRLFRERAFSA
                     GLLAQLLLASAQASFFVYLALYLQQGRGLDPLEAGLAFSILAFAYVAVSGPAPALTER
                     FGRSLVAAGGISLTLGLGSFAGVVGEIGNGGSIAVLVPSLLLVGAGIGLCLTPLTSTV
                     LSCIDPARSGAASGVLSTTQQVGYALGVAVTGVIFFGAGRAPAQGFELASVQLTAVAA
                     ALVLMTGMLPRRVTPQVAAPAG"
     gene            complement(7411..8799)
                     /locus_tag="VF517_12140"
     CDS             complement(7411..8799)
                     /locus_tag="VF517_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX8053733.1"
                     /translation="MANGNNRDAVIAALRTAAQSQPPGTRLPAVRELMSRHRAGPGTV
                     QQALAQLKREGLVTARPGQGTFVAERPLPSSPRDPSWQSVALGPPRGFSRPLAELVSL
                     PPPGVLSLSSGYLPADLQPVTLLRDALIRAARRPGIWDRLPVEGLEPLRAWFAQDLGI
                     GMSPDDVMITPGTQAALACAFRALAEPGAAVLVESPAYLGALAGARAADLRITPVPCD
                     AEGLRPDLLEEALARTGSRLLYCQPLYANPHGAVLAPSRRGDILTLLQQHGAFMIEDD
                     WARDLSIAEHAPAPLAATDDHGHVVYVRSLTKPAAPGLRIGGLAARGPAAERLRAARV
                     VDDFFVAGPLQEAALEIVGSPGWQRHLKRLRGELRRRRDALLEGLSRHLSAVSVPDVP
                     DGGLHVWVRLRDGTDDDAVARSALRAGVLVSAGRQWFPSEPDGSFLRLTFASLDPADL
                     DAAVRRLAELGL"
     gene            8861..9736
                     /locus_tag="VF517_12145"
     CDS             8861..9736
                     /locus_tag="VF517_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8053734.1"
                     /translation="MRRVAGFLADSALVPGFLGVLAFSFTLPATRVAVQDLDGTVVGL
                     GRALVAAVLAAALLRARHERLPDPSQLRRLGVVALGVIIGFPLCTSLALTLVPASHGA
                     VFVGLIPAATAVMAVLRAGERPSAPLWMAVAAGVVAVLVFAAARGAGEPRTGDLLILL
                     AVALCAVGYAEGGSLAREIGGWRVICWALVLAAPVLAPVVAWRVAETGLHGGADAWLG
                     FAYVSVVSMFLGFFVWYRGLARGGIARIGQLQLAQPVLTLTWAALILGERFGAATVVA
                     AAAVLMCAAISQRAR"
     gene            complement(9776..10483)
                     /locus_tag="VF517_12150"
     CDS             complement(9776..10483)
                     /locus_tag="VF517_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8053735.1"
                     /translation="MSGAIVVGAGPGIGLAVSRRFAREGYPVGVIARSSATLAGVQAE
                     LDSTGAETAAAAADVTDTGALTAALDAITERFGVPDVLVYNAALIQWDEFGELMAQGH
                     LDAWATNVGGAITAIGHLAPRMAARGSGTIIITGGMPTPIPEVTSLSLGKAGVRALAE
                     LLEAKFAPSGLHVATVTVAGAVAPGTDFDPDEIAERYWALHSQPPGEWEREVLYTGTP
                     ARSIGLDRRAREELSRP"
     gene            complement(10483..11979)
                     /locus_tag="VF517_12155"
     CDS             complement(10483..11979)
                     /locus_tag="VF517_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053736.1"
                     /translation="MTTPHSVQQLDRQPTDRARMLSLYVLCVGMLMIILDSTIVNVAL
                     PSIQTDLGFTQSSLAWVVNAYLIPFGGLLLLAGRLGDLVGRKRVFTAGLALFVLASAA
                     CGLASSQGMLIGARFVQGVGGALTSSVILGMIVTMFPGPREQAKAIGVYSFVAASGAS
                     LGLLAGGVLTESISWHWIFFVNLPFGVAAMFLSARLIENDTGIGIRRGADVPGALLIT
                     GGLMLAVYTIVEAVNYGWGSLHTLGLGAIAIALLTAFVARLATAKVPLMHLRILRSRN
                     VSGANAVQVLMVGGLFGMFFMGALYLQRVLGYGPTDVGLAFLPVALLIAVMSLRVTDR
                     VILRVGPRPTLITGLGLVLTGLLWFTRLPIDADYLVDVLPTMVLFGTGAGLAFPAIVS
                     LAMSGATPEDAGVASGLVNTTQQVGGALGLAVLATLSTERTESLASQGTAHAEALVDG
                     YRLALLVASGFIALGIVVASTVLEAISVADPAEQPVAEPLRPAHSEAA"
     gene            complement(12118..12627)
                     /locus_tag="VF517_12160"
     CDS             complement(12118..12627)
                     /locus_tag="VF517_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8053737.1"
                     /translation="MDASVKEAGSETQSPPVPDNLNWLLAWVSHALATETTAALERFS
                     VTPRAHCVLSSAMKGEYTQTELATAIGLDKTTMVVTIDELEAHGLAERVPSENDRRAR
                     VIRVTPAGRKLVARGEREVARIEAEVLGSLPERQRGVLVDALSALAQDRLSEPVACAH
                     APRRRAPRG"
     gene            complement(12693..13322)
                     /locus_tag="VF517_12165"
     CDS             complement(12693..13322)
                     /locus_tag="VF517_12165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX8053738.1"
                     /translation="MSRLLHISSSPRGGASESIQIANAFLESYREAHPDVDVDTWDLW
                     DGSLPAFGPAAAAAKMTVFGGGTPEGEQAAAWRDARAAFDRFDAADVLLFGVPMWNAS
                     IPYILKQLIDVISQPGTVFGVDPQTGYTGLLEGRGKKAFVVYTSAVWGPPLGSEFGTN
                     FQSTYFDDWLRWTGITDITALRFHPTLTGDADAGRRKVHEAAREAGRRG"
     gene            complement(13319..13669)
                     /locus_tag="VF517_12170"
     CDS             complement(13319..13669)
                     /locus_tag="VF517_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8053739.1"
                     /translation="MARTISRQQLQEAIEAGDVTVVETLRAEHFAQGHLPGAIHIHYE
                     AIAERAGELLPDRDAQIVTYCSNTACRNSEIAANKLTAMGYTNVRKYAEGKDDWQEAG
                     LPLEVPAAVEGAPR"
     gene            13767..14657
                     /locus_tag="VF517_12175"
     CDS             13767..14657
                     /locus_tag="VF517_12175"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8053740.1"
                     /translation="MTPELRLIRYFVAVAEEGNVTRAAERLHISQPSLSAAIKQLEEQ
                     LGVELFARRGRQIAITPAGELLRDRGRELLEHAEVVVDVVTARDRAGAGRLRLGLSPT
                     ARYGVAPELLAACAASAPATMIYTAEDTTGALIRDVARGQLDMAIAFCAPPDAAGVDQ
                     ILVREEPAVVHLPAAHPLASRPRLSLPELAEETILVAASRDSSGFTDRILGAFTELGI
                     APRTRADPYPDLGLQAVREGLGLVIYARSAFPERLAGSAFVPVDPPLTLPFQLVVREG
                     PRSAAVEAVASIARSLSEAA"
     gene            complement(14641..15444)
                     /locus_tag="VF517_12180"
     CDS             complement(14641..15444)
                     /locus_tag="VF517_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8053741.1"
                     /translation="MQIRRLGWAGIALTSADTTLVVDLIGSFGAFEPLLTGPRAPLPQ
                     PSRDADAALVTHLHEDHVDAAALAAVLKGGGPVLRPHSVPVAGTDAVGTEAGERGLLE
                     HELETDSLQPWEARTIGPFAVTALPAVDGFGDPQLSWLIEADGQRVLHAGDTLFHGSW
                     WSIALRSDPIDVAFLPINGAVCNFPHRQPPSPFAAAMDPHQAAVAAHILGAGRAVPMH
                     YDGVHAPPFYVQVDDPSGAFAREGERLGLTVETLAPGEELQLETATPPR"
     gene            15514..16119
                     /locus_tag="VF517_12185"
     CDS             15514..16119
                     /locus_tag="VF517_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8053742.1"
                     /translation="MSPRRSAAEARETRESIVAGAVAIGSREGLESLSFGRVAEAAHL
                     SKSGVSRHFSTKEELQLATLAVGRRRFREEVWEPAAHRRPGLERLRAVMESWLGFLQV
                     CPFPGGCLVTSAATEFDGRSGPVRDAVVGELALWRTMLEREIEVAVRTGELPEDTDAE
                     LLAFELGGIALATNQAVQLHGDGAAAERGARAVERLLQPSG"
     gene            16178..16897
                     /locus_tag="VF517_12190"
     CDS             16178..16897
                     /locus_tag="VF517_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8053743.1"
                     /translation="MPPTAAPALTTIAPGVKQVTVGERAMSQVYLLDHPDGPVAFDAG
                     VRGTGQAVLDAAGAPLSRVVLSHSHIDHRGGANELGAPVFCHPDEVADVKGDAGASYT
                     DFGLIRNPVVREAMPKLLALWDGGPVDVAGTVAAGDDVAGFEVIHLPGHAPGQIALFR
                     HGDRLLLAADSIYTIDMEAGRPGAPRVPHPATSWDTEMARASIRRLIGLEPASVFTGH
                     AGHFDTDVARQLEAAASFSYA"
     gene            16905..17822
                     /locus_tag="VF517_12195"
     CDS             16905..17822
                     /locus_tag="VF517_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8053744.1"
                     /translation="MLIRRLSWCGVELEAGDGSVAVIDVLEDVLPLERFLGPPAAPVR
                     RLAREGRATVGALTHLHRDHADASALARVLAPDAVVLRPPPQHGEPLEVAGTRQAEAE
                     LAEHGIRTRVVEVGEVVTHGPFTFTALHGVDAGGDPQHSWLIEVDGCRLVHAGDTMWH
                     GEWWTRALRHGAVDVAFLPINGPRVDFPHRQPASGLPGAMTPEQAAAAAAAMRARVVV
                     PIHYGLFAKPPFYEPVERPETRLRAAAEGRGLAVRVLGDGERFTVTAAAPAPAPATAP
                     APAPAPAPAPAPAPATATAAAPAASTATD"
     gene            complement(17819..18217)
                     /locus_tag="VF517_12200"
     CDS             complement(17819..18217)
                     /locus_tag="VF517_12200"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8053745.1"
                     /translation="MATVRKEAVIDASPDKVWDAVRDWGALHERLVPGFATDARVEGR
                     TRMVTFFTGMVLEERIVTVDEAERRLVWSIVNGPYEHHNGVAQVLAEEDGRARFVWTA
                     DLLPDEAEERTGEMMERGTQVAKETLEAAA"
     gene            complement(18246..19637)
                     /locus_tag="VF517_12205"
     CDS             complement(18246..19637)
                     /locus_tag="VF517_12205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053746.1"
                     /translation="MRTLLILLTGQAMAVMDGSILAVAAPSLRTSLDASDAELQLVVG
                     MYTIAFAALVVTGARIGDVIGRRRAFLVGLTGFTLASLAGGLAPNPTILIAVRAMQGV
                     AAALMTPQVLSIIQLQYTGETRARAVGAYSFILAVGVAAGQILGGALVSADVLGAAWR
                     PALLLNAPIGVLLLLGARRGLRDPGVANPRRLDLAGATLLTGALLALVLPLTFGRDSG
                     WPAWVWPSFAACGAALAAFAVVERRVRARGRAPLFDLDVLRLPGVAAGVAAVLLIMAS
                     YSGFLISLTLHLQNSLGFSPFEAGLVFACYASGFGAASLAWTRLGAGVRRRLPIAGPL
                     VMAAGVGAIGLIASGGAWPVLATAPVLVVAGVGHACGYSPLATWLTTAVRAAQAGDLS
                     GLVITGSMVGNVIGIAAFTGIYLSAGAHDSAHGLAVTTAVLGGVLVLTALCAARALVP
                     TRAAGAVPAESHA"
     gene            19706..20521
                     /locus_tag="VF517_12210"
     CDS             19706..20521
                     /locus_tag="VF517_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8053747.1"
                     /translation="MLDSRTIAAEGDIAIADVACTHERGRGETTEYADRHAVVFVRRG
                     CFSRVADGVEALLDPTTAYCMNPGEEQRYDHPHSHGDDCTAVFLPAELVGDLTRDGRL
                     PSRALPVSSAADLEHRLLVHATHEADVHEVIERSIALAALTVGHVSAPRPRARRPAAR
                     RAHAALAAGAREALADDPDASLSVLAARLAVSPHHLSRVFRAQTGKTIARHRMRLRAR
                     AALERLGGGESDLGRLAADLGFADQSHMCRVLDRETGRTPAALRHALGASSKR"
     gene            complement(20601..21185)
                     /locus_tag="VF517_12215"
     CDS             complement(20601..21185)
                     /locus_tag="VF517_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX8053748.1"
                     /translation="MTPGQDTALAIRNTLRGGRRAGVHTALGVAAGQAAWSAATAAGL
                     AALLLASEPAFLVVKVAGSLYLIGLGLGSLRRALSRRSRAHATDGRAPEKPLRAAAAF
                     RQGMVSNLGNPKMAVFFTSLLPQFVGEHASFVSPLLLGLCFCSMTALWLSAYAAVIAR
                     FGELLDRPRPRRVLDAITGMTLVALGLRLAHERR"
     gene            complement(21264..22178)
                     /locus_tag="VF517_12220"
     CDS             complement(21264..22178)
                     /locus_tag="VF517_12220"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX8053749.1"
                     /translation="MTEEQFDELRPSAFAIAYRMLGSVSEAEDVVQEGFLRLHGAIES
                     GVRIESPRAYLATVVARLALDHLRSARVRRETYVGDWLPEPLVASADDDPARRAEMAD
                     SLSLAFLVLLESLSPEQRAAFLLREVFDEPYDRIAEIVGTSEQNARQLATRARRHVEE
                     RRPRFEASREQREELAARFFAATEDGDLEGLEELLAHDVVLHGDGGGKVPALARAVHG
                     RARVARTLTAWQRAGDRLLGGVTLRREEVNGQPGALFFDGEGRLFSVMVIDVAEGQIQ
                     GVRSIVNPDKLRHLGPVANLGTLLREQG"
     gene            complement(22181..23128)
                     /locus_tag="VF517_12225"
     CDS             complement(22181..23128)
                     /locus_tag="VF517_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8053750.1"
                     /translation="MKVFVAGATGVLGRALVPRLVAEGHEVVGMTRSASKQDLVRSLG
                     ARPVVADALDADAVAQAVASAEPEVIVHQLTALSGDFDMRHIDRFFATTNRLRTEGTD
                     HLLAAALAVGARRFVAQSYAGWPAARTGARVKDEHAPFDPDPPEPLRDMLSAIRHLED
                     AVTGADSLEGVVLRYGGFYGPGTTLSADPDAAHSVAVRKRQFPVVGDGGGIWSFVHVD
                     DAAAATALAVERGARGIYNVVDDEPAPARDWLPELARVLGAKPPRRVPRWLGRLLAGE
                     AAAVMMTEVRGASNEKAKRELGWQPRYASWRQGFAQGLG"
     gene            23225..23599
                     /locus_tag="VF517_12230"
     CDS             23225..23599
                     /locus_tag="VF517_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053751.1"
                     /translation="MTAQGSPYMRLKRAIARGNPTIAMATAAELPRVPLEDALALCLL
                     LLDREPARYEPAAVRWHSRFCREARPSLADAQLALAALHALAGPSADAAAQSLLAVSE
                     SVSQDRCAEVLAAWLERRASLP"
     gene            23656..24378
                     /locus_tag="VF517_12235"
     CDS             23656..24378
                     /locus_tag="VF517_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053752.1"
                     /translation="MAKPEENLKRAGRWEILGAWLHVWTPPRGTYIPPVPTRKILLGA
                     VATAIVVAVAAVLIGRSAEEGDKREARDIAAARARSRARVAKEQKPRLKQLTAPVPSG
                     ASASELSDHRRQLLSKLETEITADAQARFRDGTLGVRPKFTACKPYVRPRVKNPPEPP
                     LSAAIGKYECLATTVEVSPTQRTEGGHAGYPFWARVDFKGGRVVWCKINLRPAEGGIG
                     HDVFVRLPAPCDLKLDGVVPKA"
     gene            24399..>24727
                     /locus_tag="VF517_12240"
     CDS             24399..>24727
                     /locus_tag="VF517_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8053753.1"
                     /translation="MATPRVSAETNVIGAMAIALADTLRTATEAAAEMPASFPAALAA
                     LRTWASGRTVDVLAEGLRVSHSRTVRVVDRLERDGLARRRPDPEDARAVRVELTPAGR
                     RAADRVLA"
BASE COUNT         3313 a         8925 c         9120 g         3369 t
ORIGIN      
        1 ctcgtgctgg tcacgggcgc ggcgttcggc atcgtctggg gtctcgttcg cggcaactcg
       61 gccggctggg ggagcgccga ggtgctgctc acgctcgtgt ccggcgtggc gctggtcggg
      121 gcgttcgcga tctgggagtc acgggcggcc gcgccgatgc tgccgctgcg cctcttccgc
      181 tcgcgtgcat tctcggcggg caacgtggcg atcttcttcc actggggctc ggccctcgga
      241 gccgtgttct tcatggccca gttccttcag gtcgttctgg gatacggccc gctgaagtcc
      301 gggttggggc tcatgccgtg gggggtgacg accgtcgtcg tcgcccaggc cgtcgggcgc
      361 cgcatcggtc gctacggcga gcgaccgttc atcgcggcgg ggttgacgct gaacgccgtc
      421 gggctgatcg ggatcgcgct gaccgccggc ccctcggtcg cctactggcc gatcgccgtc
      481 ggcctgctgc tctccggcag cggcatcgcg atgtcgatac cggcagcgca gagcgcggtg
      541 atgacatccg tggcgccccc cgacatgggc aaggcctccg gcgccttcag cacgatgcgc
      601 cagctcggcg gagcgttcgg cgtggcggtg ctcgtcgcga tcttcgcggg tgccggcagc
      661 tacgactcgc cgcaggcttt cagcgacggc ttcgtcgcgg ccgccgtcgc ctgtgcggga
      721 ctgtcggtgc tcggggtgat ggccgcgctc gcgctcccgc ggcacgcggc gagcacgcgc
      781 gagtcgttgg acgcgatgcc ggcgctccag gccgggagga cgtgagggcg gtccggcgcg
      841 ctctaggcgg aggcgatccc cgcgagcagc catcggaggc cgttctcgaa gttcgtctcg
      901 agctccgccg gagacggcag ctgcgcgtcg tcgagctcgg ggtcgatcgg tgtctgcgcg
      961 gtgaacgcgg ccgacccgat cgtgtagacg aggagcgcgc ggagcgcttc gcgcgctggc
     1021 gtgccggtga tcccctgggt ggccagcagg gcgagcatga tctccccgag ccgctgcgcg
     1081 ttggggccgc gagcgccgcg gcgggccacg tagagcggca cgagaccggg gtgctcgagc
     1141 agcacctcat gggtcgacgt catcaggccg tacaggccgc ctcgccagtc ggtgccgagc
     1201 gggtcggggg cgtccacctg tgagaggagg tcgtcgagca cctcgtcgat gagctcggcc
     1261 ttggtgcgca cgtggctgta gagcgcaccc ggcgcgacgg cgaggtgacg ggccaccgca
     1321 cgcatcgtca gcccggcggc gccatgctcg atcaggagct cgcgcccggc ggcgatcacc
     1381 agctcgcgtg tgatcccggc tcgctgaccg ggcctgcggc tcgcctccac gaactcaact
     1441 gtacaccgtc caacttaagc tgtacactgt tcaactgatc cactcgaagg agcgctccaa
     1501 tggctgacat tgccctattc ccatccgtcc tcggtgtccg cgccggtgtg ctggacgccc
     1561 aagatcggct gagccgtgca gggcacgagg tcaccgtgat cgatcagtac gacggacgct
     1621 cgttcgacga ctacgaggag gccggggcct tcgccgagtc gctcggctac gcgggactga
     1681 tggccggtgc gttcgccgcg gtcgagcggc tgccggacgg cttcatcgcg gtcggcttct
     1741 cgaacggcgg tgggatggcc gagcacgtgg cgcttcatcg cgacgtcgcg ggagtggcac
     1801 tgctcgccgg cgtgctgccg ctcgacatgc tcggcgatcg gccgtggccc gccggggtgc
     1861 cggtccagat ccactacacc cgggacgatc ccttccggcg cgaggactgg ctcgagtcgc
     1921 tgacggggag cgtccgcgca tcgggcgcga gcgtcgagct gtacgactac gccggggcgg
     1981 ggcacctctt caccgacccg tcgctgcccg ccgagtacga cgccgccgcc gccgagcagc
     2041 tctggcggcg ggtgctcgcc ttctgtgcgg cctgtgacga gacgcgccgg agcgcgtcag
     2101 accggctcga gctcggcgtt cgcccgctcc agcaccgtgc ccagtacgtg accggcggtg
     2161 gcgagatcgc ccacgggcag gtcgccccat aggcggtcgg tgacctcggt gatggcggcg
     2221 cggacccggc cgtgcagttg ccgccccgcc tcactgacct cgactagcga cccgctcccg
     2281 ccggactcga ggagccgcgc gtcggcgagg gtcgtgagat gggcccgcgc ctccgccggg
     2341 ctgaccttga gagcgccggc cacccgctgg gtcagctcgt cgcggtcgac gggtccgccg
     2401 gcggcgacgc tcaccgacag cgcgatccac gcgggctggt tcaggtcggt gccgctcagc
     2461 tgccgctcga ggatcgcgct gagggccttc tcggtctggc cgatcaccgg cgcaccgaat
     2521 gtcggaggtg cgggcatggg gcttccttcc gtcgtcgtct cgccgcgcgt gttggtgatc
     2581 cgcgtggcga tcacggccgc cgccaggagg aagacgctgc acgccagcag ggcccgctgg
     2641 aagcccgacg tgagcgcggt ggcgggctgt gcgtggctct ccagcagatc gttgatccgg
     2701 gtggtcgcga tcgccgagaa gatcgcgagt ccgagggcgc caccgacctg cgtcgaggca
     2761 ttcacgagcg ccgcggccag gcccgcccgg tcggcaggca cgcccgcgtt ggccgcggtc
     2821 gtgacggcga cgaacacggc gccgagaccg agcgacatga tgaacatgcc cggcagcagg
     2881 tcgctcacgt aggaaccgtc gacggggatc cgcgagagcc agaggacgcc gcccgccgcg
     2941 atcagcgagc cggcgatgct cacgggccgc gtcccgacgc gctccagcag ctgcgacgag
     3001 acgccggcgg ccaggccgat gccgaacgtg aggggcaggt acgacgcgcc ggcctggatc
     3061 ggcgagtagc ccagcacgtt ctgcatgtag agcgtgagga agaagaacat cgccaggacc
     3121 ccggccgagg cgatcagctg cgtggcgtca gcggcggcga ggcccttcac gcggaagatc
     3181 gagagaggga gcaggggctt cttcgcgcgg tgctcgttga tcgcgaaccc cgcgagcagc
     3241 gccagcgctc cggccagccc gaggatcgtt cggccatcgc cccagccctg gtcgggcgcc
     3301 tccaccagcg tgaagaccag cagcagcatc ccggctgtcg cgaggatcgc tccggccacg
     3361 tcgaagctgc ggcgctgcgg ctcgggccgc tcgccgtcga tcagccggaa cacgcccacc
     3421 agcaccaccg ccgcggcgat cgggttgacg aacatcaccc agcgccagcc ggggccctcg
     3481 gtgaggaagc cgccgaggag cacgccggcc gccgaggcca ggccggcgac ggcgccccag
     3541 atgccgagcg ccgtgttgcg gtcggacccc tcctggaacg tggtcgtgag gatcgacagc
     3601 gcggcgggaa ggctgaaggc ggcgccgatg ccctgggcga gccgggcagc caccaagagg
     3661 cccgagctcc cggcgaagcc acccacgagc gacgcgccac cgatcaagac cacgcccgcg
     3721 acgagcacac ggcggcggcc gaggaggtcc gcggcccggc cgccgagcag catgaagccg
     3781 ccgtaggtga gcaggtagcc gctggtcacc cactggaggc cctgctcgga gaagtggagg
     3841 tcccggcgga tcgaagggag cgcgatgttg acgatcgacg cgtcgacgaa gtcgaggaac
     3901 gcgacggcgg cgagcagggc gagcgtgagc ttgcctcgcc gggtcgcgag gagggttggg
     3961 ggcgctgctg ctgtcgccat ctctgatctc cgttcaatgg gaaactgctt gacctgggat
     4021 atattttgta gcacaaacta tatgtcgatc gtagaaacgt gcaaagctca cggtgtggaa
     4081 acgccccctc gccccgaacc gccggtcgcg gagttcgccg ggcagctcct gttccggctc
     4141 tggcgcgcgt cgcacacgcg cgtcgcggac gcgctcgcga cggtcgggct gacgccgtcg
     4201 tcgttcgccc tgctcaacgt catcggtgcg cgcgagggag cgatccagca gcagctcggc
     4261 gcggccatgg gcatcgaccc gagcacgatg gtcgcgctga tcgacgagct cgagcgggag
     4321 gggctcgcac agcggcgccc gtccccgacg gaccgtcggg cccgccggat cgtgatcacc
     4381 gcgaagggac gcaggcgccg cgagcgcgcc cggaagctcg tcgcggacac ggaggacgag
     4441 gtgctgcacg gactgacggc cgccgagcgc gacgagctgc tggcgctgct gcggcgcgcg
     4501 ctcgagggcg cgccgcccca gacgccgtgg agctccgccg agggggacta gccgcgcgcg
     4561 gtcaggtgcg cgtcgatcgc gtcgacgatc tcgcggagcc tgccgcggat tccggcgtcc
     4621 tcgaccagac cgctcggcgc gacgtcggca cgggtgatcg gcacccgcgc gcacgccgcc
     4681 tcgaccaggt cggcatgcgc gtagctcagc accttgcgca gcgagtcgtg ggcgtccgcg
     4741 ccgccgctgg gcgcggccgg cccggcgatg ttgacccacg cgaccggtgc ctcgtagatc
     4801 tcgtcgccgc cgatggtcca gtcgagcagg ttcttgaacg agccgggcag cgctccggcg
     4861 tactccggcg tcgagatcag cacggcgtcg gcggcgcgga gccggtcacg aagctggacg
     4921 acctcggcgg gtaggggatc gtggtcgtcg tcgggattga agtgggggag ggccgccatg
     4981 ccgtcgtagg tgacggcggt ccagccgtcc ggcagcacgt cgcggaccgt ccgcagcgcg
     5041 gcctcgttgg tggagccctg cctgaggctg ccgcagagca ggagcagctc gggcatcaga
     5101 tctgcctctc actcgagggc tgggcggcgc gcagtgcttc cgcggtcgcc tcgtcggcgg
     5161 ggaagaacgc ctcgatcgac agctcggaca cggtcacgtc gatcgccgtg ccgaacgacg
     5221 tggcggtgct gatgaacgcg agcgtcgcat cgccgacccg cagctgcagg ggcacggcga
     5281 tctcggcggc gtcgagatcc gggacgggcg cgcggtcccc gctcggatat tccgcgagct
     5341 cctcgtggag cgcgaacagg gcggggtcgc cgctcgcgac ggcctggcgc ccgagccggt
     5401 cgagcagatg cgcgcgccac tcgccgaggt tcgcgatccg gggagcgagg ccgtcggggt
     5461 ggaggctcag ccgcagcacg tttacgggcg gctcgaggag atggccggcg gcgccggcgg
     5521 tgagcagcgg gatcgtcgcg ttggcggcga ccatccccca gtggcggtcg accacgagag
     5581 cgggatacgg ctcgtgcccg cgcagcacgc gatcgatcgc ggcgcgtacg ggggccatct
     5641 ccgccgcgtc gaggtcccgc tgcccgtacg cgggcgcgta gccggcggcg agcagcagag
     5701 cgttgcggtc gcgcagcggc acgtcgagct gctcggagag ctgcatgacc atctccgcgc
     5761 tcgggcgcga ccggcccgtc tcgacgaagc tgacgtgacg ggcggagacc ccggcgtcga
     5821 gcgccaggtc gagctggctg atgcggcggc gctggcgcca ctcgcgcagt agggggccga
     5881 cagcgggtcg ctcggcagtg cgctgcatgc cgaccaacgt agccatgccc ggggcgcggg
     5941 tcacttacct ccgaggtcat tgtggtgggg cgcggcggcg gtcaccgttg ccgccatgac
     6001 acctccaacc agaaccaatc gcacccactg ggcggccctg cccgtgttgc ttgccggcag
     6061 cttcgtcgtc gtactcgact tcttcatcgt caacgtcgcc ctgccctcga tcgccgccga
     6121 cctcgacgcc ggcgccagct cgctcgaatg ggtcgtcgcg gcctatgggc tgaccttcgc
     6181 ggcgttcctg atcaccgccg ggaggctcgg cgacgagctc ggccgccggc gcgtgttcgt
     6241 cgtggggctc gccctgttca cgctcgcgtc caccgcatgc gggctcgccc cgagcccgac
     6301 gaccctcgtg ctcgcgaggg cgctccaggg cgtggcgggg gcgatcgtca tgccgcaggt
     6361 cctgtcgatc atcggcgtga cctaccgcga cggcgactac gtcaaggcgc tcagcgccta
     6421 cggcgtcgcc ctggggctct cggcggtcgg cgggcaggtg atcggcggag cgctcgtgca
     6481 gacagacgtc gccggcctcg gatggcgcgc gtgcttcctg atcaacgtcc cgatctgcct
     6541 cgccgcgatc gccctgacgc ccgcgctggt gcgtgagtgt cgcgctccga gccgggcgag
     6601 gatcgacctc ctgggcgcgg cgacgcttgc ccttgcgctc gtcgccgtcc tgctgcccct
     6661 gatcgagggg cgccagcacg ggtggccggc ctggacgtgg ctgtcgctcg cgatcgcccc
     6721 gcttctcctc gcgggcttcg tcgcgcgcca ggcgcggctc gcgcgccgcg gcgggagccc
     6781 gctcgtggac ctgaggctgt tccgcgagcg agctttctcg gcgggtctgc tcgcgcagct
     6841 gctgctggcc agcgcccagg cgtcgttctt cgtctacctc gcgctgtacc tccagcaggg
     6901 gcgcggcctc gacccgctcg aggcgggcct cgccttctcg atcctcgcgt tcgcgtacgt
     6961 cgcggtctcg ggtccggcgc cggccctcac ggagcgattc ggccggtccc tggtggccgc
     7021 gggcggcatc agcctcaccc tcgggctcgg ctcgttcgcc ggcgtggtgg gggagatcgg
     7081 aaacggcggg tcgatcgccg tcctggtgcc gtcgctctta ctggtcggtg ccggcatcgg
     7141 gctgtgcctc accccgctca cgtcgacggt gctcagctgc atcgacccgg cgcgatccgg
     7201 agccgcctcg ggcgtcctct ccacgacgca gcaggtcggt tacgcgctcg gcgtggcggt
     7261 caccggcgtg atcttcttcg gcgccgggcg agcgcccgcg cagggattcg agctggcgtc
     7321 ggtgcagctc acagcggtcg ccgcggcgct cgtcctgatg accgggatgc tgccgcggcg
     7381 ggtgacgccg caggttgccg cgccggcggg ttagagcccg agctcggcga gccggcgcac
     7441 ggctgcgtcg agatccgccg ggtccagcga ggcgaacgtg agccgcagga acgacccgtc
     7501 gggctcggat ggaaaccact gacggccagc gctgaccagt acgccggcgc gcagcgccga
     7561 ccgcgcgacg gcgtcgtcgt cggtaccgtc gcgaagcctc acccagacgt gcagcccgcc
     7621 gtccggcacg tccggcaccg acacggccga caggtgacga gacaggcctt cgaggagggc
     7681 gtcgcgacgg cggcgcagct cgccgcgaag gcgcttgagg tgccgctgcc agccgggcga
     7741 tcccacgatc tcgagcgccg cctcctggag cgggccggcc acgaagaagt cgtcgacgac
     7801 gcgcgccgca cgcagccgct ccgccgcggg gccgcgggcg gcgagcccac cgatccgcaa
     7861 ccccggcgcg gccggcttcg tgagcgatcg gacgtacacg acgtgaccgt gatcgtccgt
     7921 ggcggccagc ggcgcgggcg cgtgctcggc gatggagaga tcgcgcgccc agtcgtcctc
     7981 gatcatgaac gccccgtgct gctgcagcag tgtcaggatg tcgccgcgac gcgacggggc
     8041 gagcacggcg ccgtgcgggt ttgcgtacag cggctggcag tagagcaagc gggagccggt
     8101 gcgggcgagc gcctcctcga gcaggtcggg ccggagaccc tcggcgtcgc acggcaccgg
     8161 cgtgatccgg agatcggcgg cgcgggcgcc ggccagtgcc ccgaggtacg ccggggactc
     8221 gaccagcacc gccgcgccgg gctcggcgag cgcgcggaac gcgcatgcga gcgctgcctg
     8281 cgtgcccggg gtgatcatca cgtcgtcggg gctcatcccg atgccgaggt cctgggcgaa
     8341 ccacgcgcgc agcggctcga ggccctccac cggcagccgg tcccagatcc cggggcgccg
     8401 cgcggcccgg atcagggcgt cgcgcagcaa cgtgacgggc tgaagatcgg ccgggaggta
     8461 tccgctcgag agtgagagca cccccggtgg cggaagcgag acgagctccg cgagcgggcg
     8521 gctgaagccg cgcggtggcc ccagcgcgac gctctgccag ctcgggtccc gtggcgacga
     8581 cgggagcggg cgctcggcca cgaacgtgcc ctgccccggc cgcgcggtca cgagcccctc
     8641 ccgcttgagc tgcgcgagcg cctgctggac tgttcccggc ccggcgcgat gccgagacat
     8701 cagctcgcgc accgccggca gcctcgtccc gggaggttgc gattgcgccg cggttcgcag
     8761 ggcagcgatt acagcgtcgc gattgttacc attggccatg cctagtcaga gtaacgctac
     8821 tggtacggcg gcggtaacgc accccggggc gctcggcccc atgcggcggg tcgcggggtt
     8881 cctcgcggac agcgcgctgg tacccggctt cctcggagtc ctcgcgttct cgttcaccct
     8941 gccggccacg cgcgtggccg tccaggacct cgacggcacc gtcgtcgggc tggggcgcgc
     9001 gctggtggct gccgtgctcg cggccgcgct gctcagggca cggcacgagc gcctgccgga
     9061 cccctcgcag ctgcggcggc tcggggtggt cgcgctcggg gtcatcatcg gcttcccgct
     9121 ctgcacctcc ctcgcgctga cgctcgtccc cgcatcccac ggggcggtgt tcgtgggact
     9181 tatccccgcg gccacggcgg tcatggcggt gctgcgcgcg ggtgagcgcc cgtcggcgcc
     9241 gctgtggatg gcggtggccg cgggcgtggt cgcggtgctc gtcttcgcgg cggcgcgcgg
     9301 agccggggag ccgcgcacgg gggacctgct catcctcctg gccgtcgcgc tctgtgccgt
     9361 cggctacgcg gagggcgggt cgctggcgcg cgagatcggc ggttggcgcg tgatctgctg
     9421 ggcgctggtg ctggccgccc cggtgctggc gcccgtggtg gcgtggaggg tggccgagac
     9481 cgggctccac ggcggcgccg acgcgtggct cggcttcgcg tacgtgtcgg tcgtgagcat
     9541 gttcctgggc ttcttcgtct ggtaccgcgg gctggctcgc ggcgggatcg cgcgaatcgg
     9601 acagcttcag ctcgcgcagc cggtcctgac gctgacctgg gcggcgctga tcctcggcga
     9661 gcgcttcgga gccgcgacgg tcgttgccgc tgccgccgtg ctgatgtgcg cggccataag
     9721 ccagcgcgcg cgctagagct cctcgcgtgc tagggcctgc tgagctcctc gcgcgctagg
     9781 gccggctgag ctcctcgcgc gcgcgcctat ctaagccgat gcttcgcgcc ggggtgcccg
     9841 tgtagaggac ctcgcgttcc cactcgccgg gcggctgcga gtgcagcgcc cagtaccgct
     9901 cggcgatctc gtccgggtcg aagtccgtgc cgggcgcgac cgcgccggcg accgtcaccg
     9961 tggccacgtg gaggccgctg ggcgcgaact tcgcttccag cagctcggcc aaggcgcgga
    10021 cgcccgcctt cccgagcgac aggctcgtca cctcgggaat cggagtgggc atcccgccgg
    10081 tgatgatgat cgtgccgctg ccgcgcgccg ccatccgggg ggcgaggtgg ccgatcgcgg
    10141 tgatcgcccc gccgacgttg gtcgcccagg cgtcgagatg tccctgcgcc atcagctcgc
    10201 cgaactcgtc ccactggatt agcgccgcgt tgtagacgag cacgtcgggg accccgaacc
    10261 gttcggtgat cgcgtcgagg gctgccgtca gagccccggt gtcggtgaca tcggcggcgg
    10321 ccgctgcagt ctcggccccc gtgctgtcga gctccgcctg aacgccggcg agggtggcgg
    10381 acgaccgcgc gatcacgccg accggatagc cctcgcgcgc gaaccgccgg gacacggcga
    10441 ggccgatccc ggggccggcg ccgaccacga tcgcgccgga catcacgcgg cctcggagtg
    10501 cgcgggcctg aggggctcgg cgacgggttg ctcggcgggg tcggcgacgg agatcgcctc
    10561 gaggacggtc gacgcgacga cgatccccag cgcgatgaag ccggacgcga cgagcagcgc
    10621 caggcggtag ccgtccacga gcgcctcggc gtgcgccgtg ccctggctcg ccaggctctc
    10681 ggtgcgttcg gtcgacaggg tggcgagcac ggcgaggccg agcgctccgc cgacctgctg
    10741 ggtcgtgtta acgagcccgg aggcgacgcc tgcatcctcc ggggtcgccc cggacatcgc
    10801 gagcgacacg attgccggga acgccaggcc ggctcccgtg ccgaacagga ccatggtcgg
    10861 gagcacgtcg acgaggtagt cggcgtcgat cggcagccgc gtgaaccaca gcaggccggt
    10921 gagcaccagc ccgagacccg tgatcagcgt gggccgcggc ccgacccgga ggatcaccct
    10981 gtcggtgacc cgcagcgaca tgaccgcgat cagcagggcg acgggcagga acgcgaggcc
    11041 cacgtcggtc ggcccgtatc cgaggacccg ctggaggtac agcgctccca tgaagaacat
    11101 gccgaacagg ccgcccacca tgaggacctg gaccgcgttc gcgcccgaaa cgttgcggga
    11161 gcggaggatc ctcaggtgca tgagcggcac cttcgccgtg gcgagcctgg cgacgaaggc
    11221 ggtcagcagc gcgatggcga tcgctcccag cccgagcgtg tggagggagc cccagccgta
    11281 gttcaccgcc tcgacgatcg tgtagacggc gagcatcagc ccgcccgtga tcagcagcgc
    11341 gccgggcacg tcggcgcccc tgcggatgcc gatgccggtg tcgttctcga tcagccgcgc
    11401 ggagaggaac atcgccgcga ccccgaacgg gaggttcacg aagaagatcc agtgccagct
    11461 gatcgactcg gtgaggacgc cgccggcgag gaggccgagc gatgccccgg aggcggcgac
    11521 gaagctgtag acgccgatcg ccttcgcctg ctccctgggt ccggggaaca tcgtgacgat
    11581 catcccgagg atcacggagg aggtcagcgc gccgccgacg ccctggacga agcgcgctcc
    11641 gatcagcatc ccctggctcg aggcgagccc gcacgcggcc gaggcgagca cgaacagcgc
    11701 gagccccgcg gtgaagacgc gcttgcggcc gacgagatca ccgagccgcc ccgcgaggag
    11761 gagcagcccg ccgaacggga tcaggtacgc gttcacgacc caggcgagac tggactgggt
    11821 gaacccgagg tcggtctgga tcgacggcag cgcgacgttc acgatcgtgc tgtcgaggat
    11881 gatcatcagc attccgacgc agaggacgta gagggagagc atgcgcgccc ggtcggttgg
    11941 ctggcggtcg agctgttgga ctgagtgcgg ggtggtcacg gccgaccttt ctgacgggag
    12001 taccggaggg aacgaccgta acagattgtc ccgttcggga atatcttgta cgggagcata
    12061 tgtcggcaac cggccgcagc gcgccgcggg cggtcggccg gatgagagag cgcggggcta
    12121 gccgcggggg gcgcgccggc gcggcgcgtg agcgcaggcg acgggctcgg acaggcggtc
    12181 ctgagcaagc gccgaaaggg cgtcgacgag cacgccccgc tgccgctcgg ggaggctccc
    12241 gagcacttcc gcctcgatcc tggcgacctc gcgctcaccg cgggcgacga gcttccggcc
    12301 ggcgggcgtg accctgatca cccgcgcgcg gcggtcgttc tcggacggca cacgctccgc
    12361 gagcccgtga gcttccagct cgtcgatcgt cacgaccatc gtcgtcttgt cgagtccgat
    12421 cgccgtggcc agctcggtct gcgtgtactc gcccttcatg gcggacgaca gcacgcaatg
    12481 cgcgcgcggc gtgacgctga accgctccag cgccgccgtc gtctccgtgg ccagcgcgtg
    12541 actgacccag gcgagcagcc agttgaggtt gtccggcacc ggagggctct gagtctccga
    12601 tccggcttcc ttgaccgacg cgtccatggg caccgagcct aacacggtcg tgccgtttgg
    12661 gactatctat gtcggaacga ttcatcgccc cgtcagccgc gccggcccgc ctcgcgcgcg
    12721 gcctcgtgga ccttccggcg cccggcgtcg gcgtcgcccg tgagcgtggg gtggaagcgc
    12781 agcgcggtga tgtcggtgat gccggtccac cgcagccagt cgtcgaagta ggtcgactgg
    12841 aagttggtcc cgaactcgct tccgagcggc gggccccaca ccgcgctcgt gtagaccacg
    12901 aacgccttct tccccctgcc ctcgagcagg cccgtgtagc ccgtctgggg gtccacgccg
    12961 aagaccgtcc ccggctggga gatcacgtcg atgagctgct tgaggatgta ggggatgctc
    13021 gcgttccaca tcggaacgcc gaagagcagg acgtcggccg cgtcgaagcg gtcgaacgcg
    13081 gcgcgcgcat ccctccacgc cgccgcctgc tcgccctcgg gggtgccgcc cccgaagacc
    13141 gtcatcttcg ccgcggccgc ggccgggccg aacgccggca gggatccgtc ccagaggtcc
    13201 cacgtgtcga cgtccacgtc ggggtgcgcc tcccggtagc tttcgaggaa ggcgttcgcg
    13261 atctgaatcg actccgacgc gccgccacgc gggctcgagc tgatgtgcag gaggcggctc
    13321 atcgcggtgc tccctcgacc gcggcgggca cctcgagggg gagtccggcc tcctgccagt
    13381 cgtccttgcc ctcggcgtac ttgcgcacgt tcgtgtagcc catcgcggtc agcttgttcg
    13441 cggcgatctc ggagttgcgg caggccgtgt tcgagcagta cgtcacgatc tgggcgtcgc
    13501 gatccggcag cagctcgccg gcgcgctcgg cgatcgcctc gtagtggatg tggatggcac
    13561 ccggcaggtg gccctgcgcg aagtgctcgg cgcgaagcgt ctcgacgacg gtcacgtcgc
    13621 ccgcctcgat tgcctcctgc aactgctgac ggctgatggt cctggccatg ggtctctcct
    13681 gttcggcgtt tggtcgaggc cccaaggtga tgggcctcgg cgcccgcgtc caagaccgat
    13741 cgactgggtc gagataggct gcgcctatga cgccggagct gcggctcatc cgctacttcg
    13801 tggccgtcgc cgaggagggc aacgtcaccc gcgcggccga gcgtctccac atctcgcagc
    13861 cgtcgctcag cgcggcgatc aagcagctcg aggagcagct cggcgtcgag ctgttcgcac
    13921 gcaggggacg ccagatcgcg atcacgccgg ccggggagct gctgcgcgac cgcgggcgcg
    13981 agctgctcga gcacgccgag gtggtggtcg acgtggtcac cgcgcgcgac cgggccggcg
    14041 cgggccggct gcgcctgggg ctctcgccga ccgctcgcta cggcgtggca ccggagttgc
    14101 tcgcggcctg tgccgccagc gccccggcga cgatgatcta caccgcggag gacaccaccg
    14161 gcgccctcat ccgcgacgtg gcgcgcggcc agctcgacat ggcgatcgcg ttctgcgccc
    14221 cgccggacgc ggcgggcgtc gaccagatcc tcgtgcgcga ggagcccgct gtggtgcacc
    14281 tgccggcggc gcacccgctg gcgtcacgcc cccggctcag cctgcccgag ctcgccgagg
    14341 agaccatcct cgtcgcggcc agccgcgatt cgagcgggtt caccgatcgc atcctcggcg
    14401 ccttcacgga gctcgggatc gcgccgcgca cgcgagccga tccctacccc gacctcgggc
    14461 tccaggccgt acgcgagggt ctcgggctcg tgatctacgc gcgcagcgcc ttcccggagc
    14521 ggctcgccgg atcggcgttc gtgccggtcg acccgccgct gacccttccc ttccagctcg
    14581 tcgtcaggga gggcccgagg agcgcggccg tcgaggccgt ggcgtcgatc gcgcggagcc
    14641 tcagcgaggc ggcgtagccg tctcgagctg cagctcctcg cccggcgcga gggtctcgac
    14701 cgtcaggccg agcctctcgc cctcgcgggc gaacgcgccg gacggatcgt cgacctgcac
    14761 gtagaacggc ggcgcgtgca cgccgtcgta gtgcatgggg accgctcgtc cggctccgag
    14821 gatgtgggcg gccaccgccg cctggtgggg gtccatcgcc gcggcgaacg gactgggcgg
    14881 ctggcggtgc gggaagttgc agaccgcgcc gttgatcggt aggaacgcca cgtcgatcgg
    14941 atcgctgcga agcgcgatgc tccaccacga gccgtggaag agcgtgtcgc ccgcgtgcag
    15001 cacccgctgc ccgtcggcct cgatcagcca cgagagctgc ggatcgccga agccgtcgac
    15061 cgccggcagc gcggtgacgg cgaacgggcc gatcgtcctc gcctcccacg gctgcaggga
    15121 gtccgtctcg agctcgtgct cgagcagtcc ccgctcgccc gcctcggtcc cgactgcgtc
    15181 ggtcccggcg accggtacgg agtgcggccg cagcacggga ccgccgccct tcaggacggc
    15241 ggcgagcgcg gcggcgtcga cgtggtcctc gtgcagatgg gtcacgagcg cggcgtcggc
    15301 gtcccgcgac ggctgcggca gcggcgcgcg gggaccggtg agcaacggct cgaaggcacc
    15361 gaaggatccg atcaggtcga cgacgagtgt ggtgtcggca ctggtcagtg cgatacctgc
    15421 ccagccgagg cggcgtattt gcatgcggcg agttctagca cacgatcgtt ccctaaatgg
    15481 cgttcgctcg cgtgctaagg ctaagctgga cccatgagcc cgcgacgcag cgcagcggag
    15541 gcacgcgaga cccgtgagag catcgtcgcg ggcgctgtcg cgatcgggtc gcgcgagggg
    15601 ctcgagagcc tgagcttcgg cagggtggcc gaggcggcgc acctgagcaa gtccggcgtg
    15661 agccgccact tctcgacgaa ggaggagctt cagctggcca cgctcgccgt cggcaggcgg
    15721 cgctttcgcg aggaggtctg ggagccggcc gcgcatcggc gccctggcct cgagcgcctt
    15781 cgggcggtaa tggagtcctg gcttggcttt ctccaggtgt gcccgttccc cggcggctgc
    15841 ctcgtcacat cggccgcgac cgagttcgac gggcgcagcg gcccggtgcg cgacgcggtg
    15901 gtgggggagc tggcgctctg gcgcacgatg ctcgagcgcg agatcgaggt cgccgtccgg
    15961 accggcgagc tgccggagga caccgacgcc gagctgctcg cgttcgagct cgggggcatc
    16021 gcgctggcca ccaaccaggc ggttcagctc cacggcgacg gcgccgcggc cgagcgcggc
    16081 gcccgcgcgg tcgagcggct gttgcagccg tccggctgat ttagcgcacg ttcggttgct
    16141 aatgtggcgc gccccgtcca aggaaggggg gccccagatg cctccgaccg ccgcaccagc
    16201 cctcaccacc atcgcgcccg gcgtcaagca ggtgaccgtc ggcgagcgcg cgatgagcca
    16261 ggtgtacctg ctcgaccacc ccgacggccc ggtcgcgttc gacgccggcg tgaggggcac
    16321 ggggcaggcc gtgctagatg ccgccggggc gcccctgtcg cgcgtggtgc tgagccacag
    16381 ccacatcgac cacaggggcg gggcgaacga gctcggcgcc ccggtcttct gccatccgga
    16441 cgaggtcgcc gacgtgaagg gtgacgccgg ggcgagctac acggacttcg ggctgatccg
    16501 caacccggtc gtgcgcgagg cgatgccgaa gctgctcgcc ctctgggacg gcggcccggt
    16561 cgacgtggcc gggacggtcg cggcgggcga cgacgtggcg ggcttcgagg tcatccacct
    16621 gccgggccac gccccgggcc agatcgcgct gttccggcat ggcgatcgcc tgctgctcgc
    16681 ggccgacagc atctacacga tcgacatgga ggccggccgg cccggggcgc cgcgtgtgcc
    16741 gcaccccgcc acgagctggg acaccgagat ggcgagagca tcgatccggc ggctgatcgg
    16801 gctcgagccg gcgagcgtct tcacaggtca cgcgggccac ttcgacaccg acgtcgcgcg
    16861 acagctcgag gccgccgcga gcttctcgta cgcgtgagcc acggatgctg atccgccggc
    16921 tgagctggtg cggcgtcgag ctcgaggccg gcgacggctc ggtcgcggtc atcgacgtgc
    16981 tcgaggacgt gctgccgctc gaacggttcc tcggcccgcc ggccgcaccg gtcagacgcc
    17041 tggcgcgcga gggccgggcg accgtcggag cgctgacgca cctgcaccgc gaccacgccg
    17101 acgcgtcggc gctagcgcgc gttctcgcgc ccgacgcggt ggtgctccgg ccgccgccgc
    17161 agcatggcga gccgctcgag gtcgcgggga cgcgccaggc ggaggccgag ctcgccgagc
    17221 acggcatccg gacacgggtg gtggaggtcg gcgaggtcgt gacgcacggc ccgttcacgt
    17281 tcaccgcgct gcacggcgtc gacgccggcg gcgacccgca gcactcgtgg ctgatcgagg
    17341 tcgacggctg ccggctcgtc cacgccggcg acaccatgtg gcacggcgag tggtggacgc
    17401 gggcgctgcg tcacggggcc gtcgacgtcg ccttcctgcc gatcaacggg ccgagggtgg
    17461 actttccgca ccggcagccg gcgagcgggc tgccgggcgc gatgacgccc gagcaggccg
    17521 ccgccgcggc ggcggcgatg cgcgctcgcg tcgtcgtgcc gatccactac gggctgttcg
    17581 cgaagccgcc gttctacgag ccggtcgagc gccccgagac gcgccttcgc gccgccgccg
    17641 aaggccgcgg gctggcggtc agggtcctcg gcgacggcga gcggttcacg gtcaccgcgg
    17701 ccgcgccggc gcccgccccc gcgaccgcac cggcgcccgc ccccgcaccg gcgcccgccc
    17761 ccgcgccggc ccccgcgacc gcgaccgccg ccgcgcccgc ggcgtccacc gccaccgact
    17821 aggcagccgc ctcgagcgtc tccttcgcca cctgcgtgcc gcgctccatc atctcgcccg
    17881 tacgctcctc cgcctcgtcc gggagcaggt ccgctgtcca gacgaagcgc gcgcggccgt
    17941 cctcctcggc gagcacctgc gccacgccgt tgtggtgctc gtaggggccg ttcacgatcg
    18001 accagaccag ccgccgttcg gcctcgtcga ccgtgacgat ccgctcctcg agcaccatcc
    18061 ccgtaaagaa cgtgaccatg cgggtccggc cctcgacgcg ggcgtcggtc gcgaagccgg
    18121 gcacgagccg ctcgtgcagc gcgccccagt cgcggaccgc gtcccagacc ttgtccgggc
    18181 tcgcatcgat caccgcctcc ttacgtaccg tcgccatcgg atcgctcctc ctgttcgtgg
    18241 gtccgtcaag cgtgtgactc ggccggcacg gcgccggcgg cgcgggtcgg taccagcgcg
    18301 cgcgccgcac agagggccgt gagcacgagc accccgccca gcaccgcggt cgtgaccgcg
    18361 aggccgtgcg cggagtcgtg tgcgccggcg ctcaggtaga tcccggtgaa cgccgcgatt
    18421 ccgatcacgt tgccaaccat gctcccggtg atcacgaggc cgctcaggtc gccggcctgc
    18481 gccgcgcgca cggcggtggt gagccaggtc gcgagcgggc tgtagccgca ggcgtgtccg
    18541 acccccgcca ccaccagcac cggcgccgtg gccaggacgg gccaggctcc gccggaggcg
    18601 atcagcccga tcgcgcccac cccggccgcc atgaccagcg ggccggcgat cggcaggcgg
    18661 cgccgcaccc cggcaccgag ccgcgtccag gcgaggctcg cggcgccgaa gccgctcgcg
    18721 tagcacgcga acaccagccc cgcctcgaac gggctgaaac cgaggctgtt ctggagatgc
    18781 agcgtgaggc tgatcaggaa gccggagtag ctggccatga tcaacagaac ggccgcgacg
    18841 cccgccgcca cccccggcag gcgcagcacg tcgaggtcga agagaggggc gcggccgcgc
    18901 gctcggaccc tgcgctcgac gaccgcgaag gcggcgagcg ccgccccgca ggcggcgaac
    18961 gacggccaga cccatgcggg ccagccgcta tcgcggccga acgtcagcgg gagcaccagg
    19021 gcgagcaggg ccccggtcag gagagtcgcg ccggcgaggt cgagccgccg cgggttggcg
    19081 acccccgggt cgcgcaggcc gcggcgggcg cccagcagca ggagcacgcc gatcggagcg
    19141 ttcagcagca gcgcgggccg ccacgccgcg ccgagcacgt ccgcgctcac gagcgccccg
    19201 cccaggatct gcccggccgc gacgcccacg gccaggatga acgagtacgc cccgaccgcg
    19261 cgcgcccgcg tctcgcccgt gtactggagc tggatgatcg ataggacctg gggcgtcatg
    19321 agtgcggcgg cgactccctg catcgcacgg acggcgatca ggatcgtcgg gttcggcgcg
    19381 aggccgcccg ccagcgacgc aagcgtgaac ccggtgaggc cgacgaggaa cgcgcgtcgg
    19441 cggccgatca cgtccccgat ccgcgcgccg gtcacgacca gcgcggcgaa cgcgatcgtg
    19501 tacatcccca ccaccagctg cagctccgcg tcggacgcgt cgaggctcgt ccgcaggctc
    19561 ggcgcggcca ccgcgaggat cgagccgtcc atcacggcca tggcctggcc ggtcagcagg
    19621 atcaggagcg tgcgcatggc cggcaggctt ccaggcggcc gccgaacgct cttggacttt
    19681 cttgctcgtt tgcgaagatg ggcggatgct cgactcgcgg acgatcgccg ccgagggcga
    19741 catcgctatc gcggacgtcg cgtgcacgca cgagcggggg aggggcgaga cgacggagta
    19801 cgccgaccgg cacgcggtcg tgttcgtccg tcgcggttgc ttctctcgcg tggccgacgg
    19861 ggtggaggcg ctgctcgacc ccaccacggc ctactgcatg aaccccggcg aggaacagcg
    19921 ctacgaccac ccgcactcgc acggcgacga ctgcaccgcg gtgttccttc ccgccgagct
    19981 cgtcggcgac ctgacccgcg acggccggct gccctcacgg gcgctcccgg tctcatccgc
    20041 ggccgacctc gagcaccggc tgctcgtgca cgcaacgcac gaggccgacg tgcacgaggt
    20101 gatcgagcgg tcgatcgcgc tcgccgcgct gaccgtcgga cacgtgtccg cgccgcgccc
    20161 gcgcgcgcga cgccccgcgg cccggcgcgc tcacgccgcg ctcgcggccg gggcacgcga
    20221 ggccctcgcc gacgaccccg acgcctccct ctccgtcctg gcggcgcggc tcgcggtctc
    20281 cccgcaccac ctcagccgcg tgttccgcgc gcagaccggc aagacgatcg cgcgccaccg
    20341 gatgcggctg cgggcgcgcg ccgcgctcga gcgccttggc ggcggcgaga gcgacctcgg
    20401 gcggctcgca gcggacctcg ggttcgccga ccagagccac atgtgccgcg tgctcgaccg
    20461 cgagaccggt cgcacgcccg ccgcgcttcg ccacgcgctc ggcgcgtcgt ccaagcgtta
    20521 gccgagccct tcagtcgagc tgcagtcgct ggacccgctg gaggtggcgc tcgttcagct
    20581 cccgactgcg gggatggcca ctagcggcgc tcgtgggcga gacgcaggcc gagggctacc
    20641 agggtcatcc cggtgatcgc gtccagcacc cggcgcggcc gcgggcggtc gagaagctcg
    20701 ccgaacctgg cgatcaccgc ggcgtaggcg ctgagccaga gcgctgtcat cgagcagaag
    20761 cagaggccga gcagcagggg gctgacgaac gaagcgtgct cgccgacgaa ctgcggcagc
    20821 aggctggtga agaacacggc catcttcggg ttgccgagat tgctcaccat gccctgccgg
    20881 aaggccgccg ccgcccgcag tggcttctcc ggtgcgcgcc cgtccgtggc gtgggctcgg
    20941 gaccgtctgg acagagcgcg gcgcagactc cccagcccga gcccgatcag atagaggctg
    21001 cccgcgacct tgaccacgag gaaggccggc tcggacgcaa gcagcagcgc ggccagcccg
    21061 gcggccgtcg ccgcgctcca cgccgcctgg ccggccgcca cgccgagcgc ggtgtggaca
    21121 ccggcccgcc ggccgccgcg cagcgtgttg cgaatcgcaa gggccgtgtc ctggcccggg
    21181 gtcactatga ccagggcgga gacggcgagg aagaggccga tctgggagat gactcagttt
    21241 ccgtcgacga gtggcgtagc agcctagccc tgctcgcgca gcagcgtgcc caggttggcc
    21301 accggcccca ggtgccgcag cttgtccggg ttgacgatcg agcgcacgcc ctggatctga
    21361 ccctcagcga cgtcgatgac catcacgctg aacaacctgc cctcgccgtc gaagaacagc
    21421 gcacccggct gcccgttcac ctcctcgcgc cgcagggtga cgccgccgag gaggcggtcg
    21481 ccggcgcgct gccaggccgt cagcgtgcgc gccacccggg cgcggccgtg gaccgctcgc
    21541 gcgagggccg gcaccttgcc accgccgtcg ccgtggagca cgacgtcgtg ggccagcagc
    21601 tcctccagcc cctcgagatc gccgtcctcg gtggccgcga agaagcgggc cgcgagctcc
    21661 tcccgctgct cgcgcgaggc ctcgaaccgg gggcgccgct cctccacgtg gcggcgggcc
    21721 cgcgtggcga gctggcgcgc gttctgctcg ctcgtcccga ctatctccgc gatgcggtcg
    21781 tagggctcgt cgaacacctc acgcagcagg aacgcggcgc gctgctcggg cgacaggctc
    21841 tcgagcagga cgaggaaggc gagcgacagc gagtccgcca tctcggccct gcgggcggga
    21901 tcgtcgtcgg cgctcgccac gagcggctcc ggtagccagt cgcccacgta ggtctcgcgc
    21961 cggacccgcg ccgagcgcag gtggtcgagc gccagccgcg cgaccaccgt cgccaggtag
    22021 gcgcgcggcg actcgatccg cacgccgctc tcgatcgccc cgtggaggcg gaggaacccc
    22081 tcctgcacca cgtcctccgc ctcgctgacg ctgccgagca tccggtaggc gatcgcgaac
    22141 gccgagggcc ggagctcgtc gaactgctcc tcggtcatgg tcagccgagc ccctgcgcga
    22201 agccctgccg ccagctcgca tagcgcggct gccagccgag ctcgcgcttc gccttctcgt
    22261 tcgaggctcc cctcacctcg gtcatcatga ccgccgccgc ctcgccggcc aggagccggc
    22321 cgagccagcg cggcacgcgc cgcggcggct tggcgcccag cacgcgagcc agctcgggca
    22381 gccagtcccg cgccggggcg ggctcgtcgt ccaccacgtt gtagatgccg cgcgcgccgc
    22441 gctcgaccgc gagcgcggtc gccgcggcgg catcgtcgac gtggacgaag gaccagatcc
    22501 cgccgccgtc gccgaccacg gggaactggc gcttgcggac ggccacgctg tgggcggcgt
    22561 cggggtcggc gctgagcgtg gtgccggggc cgtagaagcc gccgtagcgg agcacgacgc
    22621 cttcgagcga gtccgcgccc gtcaccgcgt cctccaggtg acggatggcg ctcagcatgt
    22681 cgcggagcgg ctccggcggg tcggggtcga acggcgcgtg ctcgtccttc acgcgcgcgc
    22741 cggtgcgcgc ggccggccag cccgcgtagc tctgtgccac gaagcggcgc gcgcccacgg
    22801 cgagggcggc ggcgagcagg tggtcggtgc cctccgtgcg cagccggttc gtggtcgcga
    22861 agaaccggtc gatgtggcgc atgtcgaaat cgcccgacag cgccgtgagc tggtgcacga
    22921 tcacctcggg ctcggcggag gccaccgcct gggcgaccgc gtcggcatcg agcgcgtcgg
    22981 ccaccaccgg gcgcgctccc aggctgcgca ccaggtcctg cttcgacgcg ctccgggtca
    23041 tcccgaccac ctcgtggccc tcggccacga gccgcgggac gagcgccctg ccgagaactc
    23101 cggttgcgcc ggctacgaag accttcatgt ccgctcctct cgtctcgttt gacccttaga
    23161 cgagatgggg gcccggcctg tgacagtgcg ccggccggct tgcgatccat gcgaacatac
    23221 gttcgtgaca gcccagggct cgccctacat gaggctcaag cgggcgatcg cccgcgggaa
    23281 cccgactatc gcgatggcca cggcggccga gctgccgcgg gtgccgctcg aggacgcgct
    23341 cgcgctgtgc ctcctcctgc tcgaccgcga gccggcccgc tacgagccgg cggccgtgcg
    23401 ctggcacagc cgcttctgcc gcgaggcccg tccctcgctg gccgacgccc agctcgcgct
    23461 ggccgccctt cacgccctcg ccggcccgag tgccgacgcc gcggcgcagt cgctcctcgc
    23521 agtgtccgag agcgtgtccc aggatcgctg cgcggaggtc ctggcagcct ggctcgaacg
    23581 ccgggcgagc ctgccctgag atatgtgcgt cgggcataca ttcgtgtacg atcccggccg
    23641 tcaaaagggg gtgacatggc caagccggaa gagaacctca agcgagcggg ccgctgggag
    23701 atcctcggcg cctggctgca tgtgtggacg ccgccgcgcg ggacctacat cccgccggtc
    23761 ccgaccagga agatcctgct cggcgcggtg gccacggcga tcgtggtcgc tgtggccgcg
    23821 gtgctgatcg gcaggagcgc ggaggagggg gacaagcgcg aggcgcgcga catcgccgcc
    23881 gcccgggcgc gctcacgcgc acgcgtggcc aaggagcaga agccgcgtct caagcagctg
    23941 accgcgccgg tgccgtccgg ggcatcggcc tccgagctga gcgaccaccg caggcagctg
    24001 ctcagcaagc tggagacgga gatcaccgcg gacgctcagg cgcgcttccg cgacggcacc
    24061 ctgggcgtcc gccccaagtt cacggcctgc aagccgtacg tgcgaccgcg ggtcaagaac
    24121 ccgcccgagc ccccgctcag cgcggccatc gggaagtacg agtgcctcgc cacgaccgtc
    24181 gaggtctcgc ccacccagcg aaccgagggc ggccacgcgg gctatccgtt ctgggctcga
    24241 gtcgacttca aggggggccg ggtcgtgtgg tgcaagatca acctgcgtcc ggccgagggc
    24301 ggcatcggcc acgacgtgtt cgtccggctg cccgcgccgt gcgatctcaa gctcgacggg
    24361 gtcgttccca aagcgtgacg cggtcctagg atccggggat ggcgaccccc cgcgtctccg
    24421 ccgagacgaa cgtgatcgga gccatggcca tcgccctggc cgacacgctc cggacggcga
    24481 ccgaggcggc ggcggagatg ccggcatcgt ttcccgccgc cctcgccgcg cttcgcacct
    24541 gggcgagcgg gcggaccgtg gacgtcctcg ccgagggcct gcgcgtgtcg cactcgcgga
    24601 cggtgcgcgt ggtcgaccgc ctggagcgcg acggtctcgc ccggcgcagg ccggacccgg
    24661 aggacgcgcg cgcggtccgc gtcgagctga cccccgccgg gcggcgcgcc gccgatcggg
    24721 tgctggc
//
LOCUS       DASVPL010000228         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_222487, whole genome shotgun sequence.
ACCESSION   DASVPL010000228 DASVPL010000000
VERSION     DASVPL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222487"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VF517_12245"
     CDS             complement(<1..1453)
                     /locus_tag="VF517_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053754.1"
                     /translation="MPSGSAEARTVRAFAMNPKLDLAWMESRDSYRAKMLTLADQAAP
                     NLRGPEDPARPVDTARDLMAWPEDVGLFASLSGPRAAPARSSGSLEGAILTLIGIYGP
                     QNEYYAAKYPHVVERAPQVRQLTLSLTDTFGRVVIETFSEIAARHRVWLEAGVNMAQE
                     WQVVCADRDAFNSAVPPRLPGGQLCQEENPQKVQQLRDPFEPGRDYAYEAVTSKTSNM
                     ALVFDPAGRVVSHQAKEYLTPTEMPGLLDLVPGEVTGGLGAVKTPVGTLGFVTSKDSW
                     MPDVQAKLDQAHVDLLVQPEFFVGDLVARGTQWSPDTLLASGYSNVLRHPSFEAMALP
                     ELTGNIFNFSADAQTHFAVKPRTVGAAGGHLVGQPDSPGLVGVAPWVVPDPIRPGEPV
                     RERRARLGAAGEALAPGSGVACEEPGKPAPCENGHWEGVRWQDVEVDRRPKYRRYSGR
                     LKPTRFSAARPVAPSKNPQRNAAMAVQGPNALLA"
     gene            complement(1491..1772)
                     /locus_tag="VF517_12250"
     CDS             complement(1491..1772)
                     /locus_tag="VF517_12250"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX8053755.1"
                     /translation="MAVLDDAEIDERLGRLADGWKRDGGAIERAFKFEDFRGSVDFIN
                     RIAPVADGMNHHPDLAVSWNTVTVTLSTHSENGVTENDFQLAAEIDELE"
     gene            1784..2701
                     /locus_tag="VF517_12255"
     CDS             1784..2701
                     /locus_tag="VF517_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8053756.1"
                     /translation="MRIASLVPSATEMLFALGLGDSVVGVTHECDYPPEAAELPHLTR
                     SVVPEGLPPAEIDREVRERTERGEAIYELDREELEEVEPDLIVTQAVCEVCAVAYDDV
                     VKVAGEMEDPPQVISLDPTTLGEVLADVRRLAEAADAGDAGEQLVADLADRLDRVQAA
                     VGDVAERPGVAALEWLEPVYVGGHWVPQMIELAGGFDVLGMPGEKSRVAEWDEVAAAQ
                     PEVVVAMPCGYGAARAAEEAETYAERLAGLGARSVVAVNASAYFSRPGPRLVDGIELL
                     AHLLHPDRAPEPEFADRPIEVELEPAAPR"
     gene            complement(2698..3498)
                     /locus_tag="VF517_12260"
     CDS             complement(2698..3498)
                     /locus_tag="VF517_12260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8053757.1"
                     /translation="MEVCYRHPGRETRVSCSNCDRPICPDCMTSTSVGMRCPECARQK
                     TKVKTMATIEQPTVTFVLMGISIALGLGGFLAGDPQFGGSLLDDDFAISRATVGDGEL
                     WRLVTSGFLHYGLFHLGANMLSLYILGSMLEPSLGRVRFALVYFVSLLAGSFGALLLQ
                     PVGFTAGASGAVFGLFGAAVVIFRKRGISLMESGLGLWLGLNLLISLRPGISLGGHLG
                     GFAGGLVATYVLMELQERLPVRARRAASYAIVAALGLIAIAGAVAVSA"
     gene            complement(3533..>3851)
                     /locus_tag="VF517_12265"
     CDS             complement(3533..>3851)
                     /locus_tag="VF517_12265"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX8053758.1"
                     /translation="VTASVDRTRLPGGSARLEVADADGRPATLRVSGLPVPKSGEVYE
                     VWVERDGEVRPAGALFEPGRDGHGAAAIPGGVEGVDRVMVTRERAGGVDRPTETPVIV
                     ADV"
BASE COUNT          535 a         1423 c         1391 g          502 t
ORIGIN      
        1 acgccagcag cgcgttcggc ccctgcacgg ccatcgcggc gttgcgttgg gggttcttcg
       61 acggtgcgac cgggcgcgcg gccgagaacc gcgtcggctt gagccgcccg ctgtagcggc
      121 ggtacttcgg gcgccggtcc acctccacgt cctgccagcg cacgccctcc cagtgcccgt
      181 tctcgcacgg cgccggcttg cccggctcct cgcaagcgac gcccgagccc ggcgccagcg
      241 cctcgcccgc ggccccgagc cgcgcgcggc gctcccggac cggctcgccc gggcggatcg
      301 ggtcgggcac gacccacggc gcgaccccca cgagccccgg cgagtcgggc tggccgacca
      361 ggtgcccgcc cgcggccccc acggtccgcg gcttgaccgc gaagtgcgtc tgcgcgtcgg
      421 ccgagaagtt gaagatgttg ccggtcagct ccggcagcgc catcgcctcg aacgacgggt
      481 ggcgcagcac gttcgagtag cccgaggcga gcagcgtgtc gggcgaccac tgagtcccac
      541 gtgcgaccag gtccccgacg aagaactccg gctgcacgag caggtccacg tgggcctggt
      601 cgagcttcgc ctggacgtcg ggcatccagg agtccttgct cgtgacgaag ccgagcgttc
      661 ccaccggggt cttcaccgcg ccgagtccgc cggtcacctc gcccggcacg aggtcgagca
      721 gccccggcat ctcggtcggc gtcagatact ccttcgcctg gtgcgatacc acccggcccg
      781 ccgggtcgaa gacgagcgcc atgttcgacg tcttcgaggt gaccgcctcg tacgcgtagt
      841 cgcggcccgg ctcgaacgga tcgcggagct gctggacctt ctgcgggttc tcctcctggc
      901 agagctgccc gcccggcagc cgcggcggca cggcggagtt gaacgcgtcg cggtcggcgc
      961 acaccacctg ccactcctgg gccatgttca ccccggcctc gagccacacg cggtggcgcg
     1021 cggcgatctc cgagaaggtc tcgatcacca cgcggccgaa cgtgtccgtg agcgagagcg
     1081 tgagctggcg cacctgcggc gcgcgctcga ccacgtgcgg gtacttcgcg gcgtagtact
     1141 cgttctgcgg cccatagatc ccgatcagcg tgaggatcgc gccctcgagc gagcccgacg
     1201 acctggcggg cgcggcgcgc ggaccgctca gcgaggcgaa cagcccgacg tcctcgggcc
     1261 aggccatcag gtcgcgcgcg gtgtcgaccg ggcgggccgg gtcctcgggc ccgcgcaggt
     1321 tcggcgccgc ctggtcggcg agcgtgagca tcttcgcgcg gtagctgtcc cggctctcca
     1381 tccaggccag gtcgagcttc gggttcatcg cgaacgcgcg caccgtccgc gcctcggcgg
     1441 acccggacgg caccacgccc agcgctacga cgagcgccgc gagcgcggtc ctactccagc
     1501 tcgtcgatct ccgccgcaag ctggaagtcg ttctcggtca ccccgttctc ggagtgcgtg
     1561 gagagggtga cggtgacggt gttccacgag accgccaggt cggggtggtg gttcatgccg
     1621 tcggccacgg gcgcgatgcg gttgatgaag tcgaccgagc ctcggaagtc ctcgaacttg
     1681 aacgcgcgct cgatggcgcc accgtcccgc ttccagccgt cggccaggcg ccccagccgc
     1741 tcgtcgatct cagcgtcgtc cagaactgcc atcgtttccc ttcgtgcgaa ttgccagcct
     1801 tgtcccgtca gcgaccgaga tgctgttcgc cctcggcctc ggtgactccg tcgtcggggt
     1861 cacccacgag tgcgattatc cgccagaggc ggcggaactg ccccacctga cccgcagcgt
     1921 ggtccccgag gggctcccgc cggccgagat cgaccgcgag gtgcgcgagc ggaccgagcg
     1981 cggcgaggcc atctacgagc tcgaccgcga ggagctcgag gaggtcgagc cggacctgat
     2041 cgtgacccag gccgtgtgcg aggtctgcgc ggtcgcgtac gacgacgtgg tgaaggtcgc
     2101 gggagagatg gaggaccccc cgcaggtcat ctcgctcgac ccgaccaccc tcggcgaggt
     2161 gctggccgac gtgcggcggc tggccgaggc ggcggacgcg ggcgacgcgg gcgagcagct
     2221 ggtggcggac ctcgccgacc gactcgaccg cgtccaggcg gccgtgggcg acgtggccga
     2281 gcggcccggc gtcgcggcgc tcgagtggct cgagccggtg tacgtcggcg gccactgggt
     2341 cccgcagatg atcgagctgg ccggcggctt cgacgtgctc gggatgcccg gggagaagtc
     2401 gcgcgtggcc gagtgggacg aggtcgcggc ggcgcagccc gaggtggtgg tcgcgatgcc
     2461 gtgcggctac ggcgccgcac gggcggccga ggaggcggag acgtacgcgg agcggctggc
     2521 cgggctcggc gcgcgcagcg tggtggcggt gaacgcctcg gcgtacttct cacggccggg
     2581 cccgcggctc gtcgacggca tcgagctgct cgcgcacctg ctccaccccg accgcgcgcc
     2641 ggagccggag ttcgccgacc gcccgatcga ggtcgagctc gagccggccg ctccgcgcta
     2701 ggcggagacg gccaccgcgc ccgcgatcgc gatcaggccg agcgcggcca cgatcgcgta
     2761 cgacgccgcg cggcgcgcac gcaccggcag gcgctcctgg agctccatca gcacgtaggt
     2821 ggccacgagg ccgccggcga acccgccgag gtgaccgccc agcgagatcc ccggccggag
     2881 gctgatcagc aggttcaggc cgagccagag gccgagcccg ctctccatga gcgagatgcc
     2941 gcgcttgcgg aagatcacca ccgccgcgcc gaacaggccg aagaccgcgc ccgacgcccc
     3001 cgccgtgaac ccgaccggct ggagcagcag cgcgccgaac gagcccgcga gcagcgacac
     3061 gaagtagacg agggcgaacc gcacccggcc gagcgacggc tcgagcatcg agccgaggat
     3121 gtagagcgag agcatgttcg cgccgaggtg gaacaggccg tagtggagga acccgctcgt
     3181 gacgagccgc cagagctcgc cgtctccgac cgtggcccgc gagatcgcga agtcgtcgtc
     3241 gagcaggctg ccgccgaact gcgggtcccc cgccaggaac ccacccaggc ccagcgcgat
     3301 cgagatcccc atcaggacga aggtgaccgt cggctgctcg atcgtcgcca tcgtcttgac
     3361 cttcgtcttc tggcgcgcgc actccgggca gcgcatcccg acggacgtcg aggtcatgca
     3421 gtcggggcag atcgggcggt cgcagttcga gcacgagacg cgcgtctcgc ggcccggatg
     3481 gcggtagcag acctccatcc gcgagacctt atccccgccc tctgtggcgg ctttaaacgt
     3541 cggcgacgat gaccggcgtc tcggtcgggc ggtcgacgcc gcccgcgcgc tcgcgcgtga
     3601 ccatcacccg gtcgacgccc tcgacgccgc ccgggatcgc cgccgcgccg tggccgtcgc
     3661 ggccgggctc gaacagcgcg ccggcgggac gcacctcgcc gtcgcgctcg acccagacct
     3721 cgtagacctc gccgctcttc gggaccggaa ggccgctcac gcgcagcgtc gccgggcggc
     3781 cgtcggcatc ggccacctcg agccgcgcgg agccgccggg cagccgcgtg cggtcgacgc
     3841 tggcggtcac g
//
LOCUS       DASVPL010000229         4247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_339751, whole genome shotgun sequence.
ACCESSION   DASVPL010000229 DASVPL010000000
VERSION     DASVPL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339751"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VF517_12270"
     CDS             complement(<1..311)
                     /locus_tag="VF517_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8053759.1"
                     /translation="MTIGQRLREARLRRELDIADAERYTKIRAKYLRALENDEFGMLP
                     DRTLAKTFLRTYSDYLGLDAQLLIEEYRAQHEPRGEGDLHPLAPRRAGEPRQRRAGPP
                     VG"
     gene            complement(348..2765)
                     /locus_tag="VF517_12275"
     CDS             complement(348..2765)
                     /locus_tag="VF517_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX8053760.1"
                     /translation="MARPKTVASKRRKPAAKKKSAPPQLNLPKLPKLPELEQRHHDLI
                     GLGLVALGAFFAFVFYLDWEGGKVGGALADAFVFLFGGVGYLVPVVLFAAGAVLVLKP
                     MLPSVNPFRAGGACLLAALMLGLAGGLFGLGPDDAARDGWMKPGYIEDHGGAFGEALY
                     WVASTLFQDVGAHIIFVFLLLAGVLLLTGASVAGVLRGTRAGLVGTTQRVRRSTGEFA
                     AVVTGRERRPAPEPSVGPEREPDPIVPEPDGEPVVRATHVEAPPIYGEESEEIDFAPG
                     PEPDPIAEAHYAEDEPEPEPQPEDLTPQGNRRSELTESDELDYRPPRPGLLERSKQGA
                     RVDTAAQERTAGELTEALSHFGIESQIVGAVVGPHVTRYELRLAPGVKMSKVSQLRDD
                     LAYALAATDVRILAPIPGKQAVGVEVPNEDRKMVHLGDVFQEAPAGWSPLTVWLGKDI
                     AGKAIGTDLAKQPHVLVAGTTGSGKSGCVNAMLSSILLRATPNEVRLVLVDPKQVELN
                     HYEHVPHLLTPVVTSPRLAANVLSNLIKEMESRYAVMSKARARNLVELNRIREREGER
                     PLPYILCVIDELADLMMVAPGEVEDSIIRLAQKSRAVGIHLLLATQRPSADIITGMIK
                     ANVPARIAFAVSSQTDSRVILDQNGAESLLGQGDMLFRPASESRPARIQGAFISEAEI
                     EKITDHWRMQGEPEIQEELLEAVETEDDHGADEFSADQDDLLGEAIATVVQLGSASTS
                     MLQRRLRVGYTRAGRLIDMMERRGVISGYEGSKARQVLISEADLPRVLAALDEPVGAA
                     AGVGDRG"
     gene            complement(2829..>4247)
                     /locus_tag="VF517_12280"
     CDS             complement(2829..>4247)
                     /locus_tag="VF517_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053761.1"
                     /translation="HHNITLMVLAVSALAYALSQTMVAPALPQIQHDLHTSATSVTWV
                     LTVYLLTASVATPIVGRLGDMFGKERMLLVVLGFFALGSLVAALSHSVALLVAGRAIQ
                     GIGGAVFPLAFGIIRDEFPREKVATAIGLISATFGIGGGAGLVLSGAIVDNLSYEWIF
                     WLALVVVLVAMVATRLFVPESPVKSPARIDWVGAGLLSGGLVALLAAVSEGNRWGWGS
                     PGILLLFATAAVLLVALVRYELAFDEPLVDMRMMRARPVLTTNITALLIGFGMFGSFI
                     LIPQLVQTPESTGFGFGATVTEAGLFMLPSTAVMLVAGPISGALGSRLGSKLPLLIGT
                     LMASSAFGFLAIVHDQRWAIYVGTALMGLGIGFSFAAMANLVVEAVDQRQTGIASGMN
                     TIMRTIGGSIGGQLAASVVAGHVANGQPTSTGFTIAFTLSSVVVLGAFLCALAIPARR
                     RERADVIPLSPAARPAEQRAAS"
BASE COUNT          627 a         1519 c         1521 g          580 t
ORIGIN      
        1 cccaccggcg gcccggcgcg ccgctggcgc ggctcacccg cgcggcgcgg ggcgagcgga
       61 tgaaggtctc cctcgccgcg cggctcgtgc tgggcgcggt actcctcgat caggagctgg
      121 gcgtcgagcc ccaggtagtc cgagtaggtg cgcaggaacg tcttggcgag cgtgcggtcg
      181 ggaagcatcc cgaactcgtc gttctccagc gcacgcaggt acttggcacg gatcttcgtg
      241 taccgctcgg catccgcgat gtcgagctcg cgcctcaggc gcgcctcgcg gagtcgctgg
      301 ccgatggtca cgccgcgtat tcaagcggac cgtgggcggt gcgccgtcta gccgcgatcg
      361 ccgacgccgg cggccgcgcc gaccggctcg tccagcgccg ccagcacgcg cggcaggtcc
      421 gcctcgctga tcagcacctg ccgggccttg gagccctcgt aaccggagat cacgccgcgc
      481 cgctccatca tgtcgatcag ccggcccgcc cgcgtgtagc cgacccgcaa ccggcgctgg
      541 agcatcgagg tcgaggcgct gccgagctgc accaccgtcg cgatcgcctc gcccagcagg
      601 tcgtcctggt cggcgctgaa ctcgtcggcg ccgtggtcgt cctcggtctc gacggcctcc
      661 agcagctcct cctggatctc cggctcgccc tgcatgcgcc agtggtcggt gatcttctcg
      721 atctcggcct cgctgatgaa cgcgccctgg atgcgcgccg ggcgcgactc cgaggccggc
      781 cggaacagca tgtcgccctg accgagcagc gactcggcgc cgttctggtc gaggatcacg
      841 cgggagtcgg tctgcgacga gaccgcgaac gcgatccgcg ccggcacgtt cgccttgatc
      901 atgccggtga tgatgtccgc gctcgggcgc tgggtggcga gcagcaggtg gatgccgacc
      961 gcgcgcgact tctgcgcgag cctgatgatc gagtcctcga cctcgccagg ggcgaccatc
     1021 atcaggtcgg ccagctcgtc gatcacgcag aggatgtagg gcagcgggcg ctcgccctcg
     1081 cgctcgcgga tccggttcag ctcgacgagg ttgcgcgcgc gggccttgct catcaccgcg
     1141 tagcgcgact ccatctcctt gatcagattg gacagcacgt tggccgcgag ccgcgggctc
     1201 gtgacgaccg gggtgagcag gtggggcacg tgctcgtagt ggttcagctc gacctgcttc
     1261 gggtccacga gcaccaggcg gacctcgttc ggtgtggcgc gcagcaggat cgaggacagc
     1321 atcgcgttca cgcagccgga cttgcccgag ccggtggtac cggccaccag cacgtgcggc
     1381 tgctttgcga ggtcggtgcc gatcgccttc ccggcgatgt ccttgccgag ccagaccgtg
     1441 agcggcgacc agcccgccgg ggcctcctgg aagacatcgc ccaggtggac catcttgcgg
     1501 tcctcgttgg gcacctcgac gccgacggcc tgcttgcccg ggatcggcgc gaggatgcgc
     1561 acgtcggtgg cggccagcgc gtaggcgagg tcgtccctga gctgggagac cttcgacatc
     1621 ttcacgccgg gggccaggcg cagctcgtag cgggtcacgt gcgggcccac gaccgcgccc
     1681 acgatctgcg actcgatccc gaagtggctg agcgcctcgg tcagctcgcc ggcggtgcgc
     1741 tcctgagcgg cggtgtccac gcgggcgccc tgcttggagc gctcgagcag cccgggccgc
     1801 ggcgggcggt agtcgagctc gtcgctctcg gtcagctcgg agcgacggtt gccctgcggc
     1861 gtcaggtcct ccggctgggg ctcgggctca ggctcgtcct cggcgtagtg cgcctccgcg
     1921 atcgggtccg gctcgggccc cggggcgaag tcgatctcct ccgactcctc gccgtagatc
     1981 ggcggagcct ccacgtgggt ggcgcggacc accggctcgc cgtcgggctc gggcacgatc
     2041 ggatccggct cccgctctgg cccgacgctc ggctccggcg cgggccggcg ctcgcggccg
     2101 gtcacgaccg cggcgaactc gccggtcgag cgccgcaccc gctgcgtggt gccgacgagg
     2161 cccgcgcgcg tgcctcggag aaccccggcc accgacgccc cggtgagcag cagcacgccc
     2221 gcgagcagca ggaacacgaa gatgatgtgg gccccgacgt cctggaacag cgtgctcgcc
     2281 acccagtaga gcgcctcgcc gaacgcgccg ccgtggtcct cgatgtagcc gggcttcatc
     2341 caaccgtcgc gggccgcgtc gtccggcccg aggccgaaca ggccgccggc gagcccgagc
     2401 atcagcgcgg cgagcaggca cgccccgccc gcgcggaacg ggttgaccga gggcagcatc
     2461 ggcttcagca cgagcaccgc gcccgccgcg aacaggacca ccggcacgag gtagccgacg
     2521 ccgccgaaca ggaacacgaa cgcgtcggcc agggcgccgc cgaccttgcc gccctcccag
     2581 tccaggtaga agacgaacgc gaagaaagcg ccaagcgcga ccaggccgag tccgatcagg
     2641 tcgtgatggc gctgctcgag ttcggggagc tttggcagct tcggcaggtt gagctgcggg
     2701 ggcgcggact tcttcttggc ggccggcttg cgccgcttgc ttgcgactgt ctttgggcgt
     2761 gccatgcgcg tggagagttc gacgcgccac gacgagtccc ttgcccgctc cgggctgcgc
     2821 cgcgagagct agctggcggc gcgctgctcg gccggccggg cggcgggcga aagcgggatc
     2881 acgtccgccc gctcgcgcct gcgggccggt atcgccaggg cgcagaggaa cgcgccgagg
     2941 accaccacgg acgagagcgt gaacgcgatc gtgaagccgg tcgaggtcgg ctggccgttc
     3001 gcgacgtgcc ccgcgaccac gctcgccgcg agctggccgc cgatcgagcc gccgatcgtg
     3061 cgcatgatcg tgttcatccc gctggcgatg ccggtctggc gctggtcgac cgcctcgacc
     3121 accaggttcg ccatcgccgc gaacgagaag ccgatcccga gccccatcag cgcggtgccc
     3181 acgtagatcg cccagcgctg gtcgtgcacg atcgcgagga agccgaaggc ggacgaggcc
     3241 atcagggtgc cgatcaggag cggcagcttc gagcccaggc gcgagccgag cgcgccagag
     3301 atcggtcccg cgacgagcat caccgcggtc gagggcagca tgaacaggcc cgcctccgtg
     3361 acggtcgccc cgaagccgaa gccggtgctc tccggcgtct gcacgagctg cgggatcagg
     3421 atgaacgagc cgaacatccc gaacccgatc aggagcgcgg tgatgttggt ggtcaggacc
     3481 ggccgcgcgc gcatcatccg catgtccacg agcggctcgt cgaacgccag ctcgtagcgg
     3541 accagcgcga ccagcagcac ggccgccgtc gcgaacagga ggaggattcc cggcgagccc
     3601 cagccccagc ggttgccctc gctcaccgcc gccagcagcg cgaccagccc gccggacagc
     3661 aggccggcgc cgacccagtc gatccgcgcc gggctcttca caggggactc cggcacgaac
     3721 aggcgggtgg cgaccatcgc gacgaggacc acgacgagcg cgagccagaa gatccactcg
     3781 taggagaggt tgtcgacgat cgcgccgctc agcaccaggc cggcgccgcc gccgatgccg
     3841 aacgtggcgc tgatcaggcc gatggcggtg gccaccttct cgcgcgggaa ctcgtcgcgg
     3901 atgatcccga acgccaacgg gaagacggcg ccgccgatgc cctggatcgc gcgcccggcg
     3961 accagcaggg caaccgagtg cgacagcgcc gcgaccagcg agccgagcgc gaagaatccg
     4021 agcaccacga gcagcatgcg ctccttgccg aacatgtcgc cgagccggcc gacgatcggt
     4081 gttgcgaccg acgcggtcag caggtacacg gtgagcaccc acgtgaccga cgtcgccgag
     4141 gtgtgcaggt cgtgctggat ctgcggcagt gccggggcga ccatcgtctg cgagagcgcg
     4201 taggccagtg cggacacggc caggaccatc agcgtgatgt tgtggtg
//
LOCUS       DASVPL010000230        25570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_144572, whole genome shotgun sequence.
ACCESSION   DASVPL010000230 DASVPL010000000
VERSION     DASVPL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 25570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25570
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144572"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VF517_12285"
     CDS             <1..512
                     /locus_tag="VF517_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053762.1"
                     /translation="CRVAMGRAATASKDFPGGPEDGTGTVSSGGREWEFQHVSIGNPQ
                     CAIEVGDELTELDLSVLGPPIEGDTELFPNRTNVSFWRRDGDAAITARIFERGVGETM
                     SSGTGASGAAVAAVLRGVASPVTVHLDGGELEVEVGEDLNVNLTGWAVPVYEGRLSEN
                     FVEELREAE"
     gene            499..1662
                     /locus_tag="VF517_12290"
     CDS             499..1662
                     /locus_tag="VF517_12290"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX8053763.1"
                     /translation="MKPSSRLERIPPYLFAELERKIAAKKEQGVDVISLGIGDPDTPT
                     PALVVDALAAGGADPATHQYPSNRGRADFREAVARFYERRFGVTIDPATEVIPALGAK
                     ECVFNLNLAFLDPDSVALASDPGYPVYTGGPLLAGAEAVLLPLRADAGFAPDFDSISA
                     EDWARARLMFLNYPNNPTGAVLPDGVFERAIELAHEHDLLVVHDASYTETTFDGYRAP
                     SFLEWDGAREVGVEVFSLSKGYNMTGWRTAAIAGNAEAIEHYWRLKSNIDSGMFEAVQ
                     LAAIAALDEGDATARGMSEVYARRRDLVCDALAQIGVDVTPPKGTIYIWAPVPEGHTS
                     ASYCELVLEESGVVLSPGGAYGPNGEGFFRISLTVPDERLLEAVERLRSSLGG"
     gene            1842..2780
                     /locus_tag="VF517_12295"
     CDS             1842..2780
                     /locus_tag="VF517_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053764.1"
                     /translation="MNTGSGWATWNEGGTFVDNYVRESAAARVVPVFTYYMIRQSLPG
                     RDDGDEPRAVLSNLRNASTMRAYLDNLRLFFERAARFPKTRIVLHVEPDMWGYGHQVA
                     KNDDAASVPVAVVGDLGGLAREVVRLRDEIAPNVVLGWHVSGWGTGIDLQVNDPSTKQ
                     SSALGARAARFYRSLGARFDITFTDWSDRDAGFKERILGRTHADAWFTPGDYTRWRYF
                     MSAYSRKTGQRIVVWQIPLGNTALDDSWGRYRDRHVQWLIGAGYGSRLKAMRSAGVSA
                     LLFGGGADGTTSAKTDGGYFRKRARTYYRTGPLRLP"
     gene            complement(2825..3796)
                     /locus_tag="VF517_12300"
     CDS             complement(2825..3796)
                     /locus_tag="VF517_12300"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8053765.1"
                     /translation="MRAFVTGGTGFIGQRLVSKLRERGHDVVALVRNPDKAADLKRAG
                     VELVEGDLTNVEAIRRGAEGADACFHGAAIYKVGIPKSQHAEMYETNVRGTERVLDAA
                     IQAAVPKIVYLSTINVFGNTGGEIVDESYERPPNGFLSYYDQTKFESHQVALDRIGKG
                     GPVVIVQPGGVYGPDDHSEIGNMIEQFRAGKLPMLPFPQTGLNMVHVDDVAEGCILAY
                     EHGEPGESYVLGGEITTMRGIVEKVAQIDGKKPPKRNLPTGVMKAIAPAGPLVGKLMG
                     MAPNMRELISASDGVTYWARDDKARTRLGYAPRDLETGLRQTLAASG"
     gene            3829..5184
                     /gene="hflX"
                     /locus_tag="VF517_12305"
     CDS             3829..5184
                     /gene="hflX"
                     /locus_tag="VF517_12305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX8053766.1"
                     /translation="MNRGRDGRRSETQYGDGIRNPRARQRAFCIAVAPDEPDLSELRE
                     LLLTAGVAVAGEMVQRRERPDPDRYFGKGKRAELKRAVQEADANLVACDDELLPRQER
                     NLEEELGVPVIDRTAVILDIFADHAVSAEGKLQVELAQLEYNLARMRGLWTHLERLGA
                     GRVDGGIGTRGPGESQIETDRRLARDRITAIRRRLDRTRERRDTQRGERERAHLPTVA
                     LAGYTNAGKSTLLNGLTGAEAQVRDRLFHTLDPLTRSFEIAGRQYLLTDTVGFIRKLP
                     HQLVDAFASTLEETLRADLIVHVVDASAPEDEQTEMIGAVEEVLTEIGAGEQPTVLAL
                     NKVDLLDEERRRELSFRYPRGVQVSAVTGDGLDELRDAVEAQFLSTLRPMELLLPYGE
                     GGSLSALHDAAGEVEREDTVEGVRVKARVPARVAARFERFEVNGNGASPNGGGSVGPR
                     E"
     gene            5181..5612
                     /gene="dut"
                     /locus_tag="VF517_12310"
     CDS             5181..5612
                     /gene="dut"
                     /locus_tag="VF517_12310"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX8053767.1"
                     /translation="MTLRFTRLHAEARPPAQAHEGDAGHDLHAAEPATLGPGERASVG
                     TGIAVAIPEGCAGLVLPRSGLAARHGVTLVNAPGLIDSGYRGEVRVLLLNTDRDETFE
                     VSAGDRIAQLVLVRFEAGQAEEVESLDATARGAGGFGSTGR"
     gene            5733..6191
                     /locus_tag="VF517_12315"
     CDS             5733..6191
                     /locus_tag="VF517_12315"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX8053768.1"
                     /translation="MSRHTSSSTRIARRPAPHLRASDADRDRAVDMLRDHAAAGRLST
                     EELEDRTDSALAATTFGQLDAVLSDLPRQRDPRRAERRHDLARKAFATHLRTYVMVMV
                     LLVAIWALTGMGYFWPVWPALGWGIGIASHGAYTPRGRRGHGQPRAMARP"
     gene            complement(6284..7234)
                     /locus_tag="VF517_12320"
     CDS             complement(6284..7234)
                     /locus_tag="VF517_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053769.1"
                     /translation="MMRSIGIVGALLAAAVFVAGCVSTANVAGVSNTCTNPATMNDGF
                     FANRSVYFRISTQADPVDPSTNWVCYRIKVPNQPETAGRLDVNATTDVAPVARVTTDV
                     DSRACTTTVGNTLPTTHPIQQGTVAETPFYIDAYGAGSQTWLCVEAGPVKHRVLVNAS
                     AGLNEPDVTANSDTAPGPIADTTPPPAGQPSTSCSAGAYGPATENVNAHLADRDLFLY
                     NAKPADNEVHVCARLSSPQGAAGFHLGVKAAVNQVVDVQQSSDITPCTNDVLVLGNPP
                     LALRTSPVGQTPVSVCVNSTRYTVVTGPVPPVVTFERDTQ"
     gene            complement(7362..8084)
                     /locus_tag="VF517_12325"
     CDS             complement(7362..8084)
                     /locus_tag="VF517_12325"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053770.1"
                     /translation="MTRALRVCSLYPDLMNIYADRGNIAVLRARCEWRGIGFELAASS
                     VGESVDPAAHDLFYMGGGQDRDQAAVAHDMVATKRDALHAAADRGAVVLAVCGGYQLL
                     GSSYEMGDEELPGIGLADLRTVREPGERLIGNCAIEADLGGGTRVVAGFENHGGRTYL
                     GAGEKPLGRVLSGHGNNGRDDHEGVHKGNVIGTYLHGPLLPKNVWLADRLIELALGIE
                     LDPLDDTMEDAAHMSARRAAGV"
     gene            complement(8081..8824)
                     /locus_tag="VF517_12330"
     CDS             complement(8081..8824)
                     /locus_tag="VF517_12330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8053771.1"
                     /translation="MKAAPVADPVVWHDVECAAYAADLPLWHELAAAADGPILDLGAG
                     TGRVALELAGAGHDVTALDSEPAFLRALAARARERGLRVRTEVADARSFALGREFALA
                     IAPMQVVQLLGGPSGRLRMLGSVREHLTVGGRFAAALADPFEGIPAEDAAPPVPDVRE
                     QDGWVFSSTPIAVRAEAGATVIDRHRQSVSPEGRLEEVVASIRLDDAPAEELEHLGAS
                     VRMRTLPRRHVPATGDYVGSTVVLLEKLP"
     gene            complement(8877..10232)
                     /locus_tag="VF517_12335"
     CDS             complement(8877..10232)
                     /locus_tag="VF517_12335"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX8053772.1"
                     /translation="MLRLAVAVARLARAAIRRAGRGGTTVPGRVLLRLDPRAIAHLGR
                     RIAGGVTLISATNGKTTTASMLAGVLERDERTVVRNRAGSNMHWGVATALIDAGREPG
                     ELGLFEVDEAWLARIAEQLDPGLLILGNLFRDQLDRYGELELLADRWADLVAARGDRT
                     ALVLNADDPLVADLGREHSRVTYFGVEDDTLALPELQHAADSKHCRNCGHPYVYDAIY
                     MGHLGRYRCTNCGRTRPVPEVVATRVALRGMDGSDIALRTSGGSVDVRLPLPGLYNVY
                     NALAAATAALELGVSLERVREGLEGMSAVFGRVERLSVSGRDVSILLVKNPVGANEVL
                     RTLTLEDGRIDLWLALNDRIADGRDISWIWDADFELLAGRVRRVTCSGTRAEEMALRL
                     KYAGIDAPIAVERDLERSLDAAVADRDGGTPLYALPTYTALLDLRDLLADRGVAGRWS
                     E"
     gene            complement(10232..11119)
                     /locus_tag="VF517_12340"
     CDS             complement(10232..11119)
                     /locus_tag="VF517_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8053773.1"
                     /translation="MADPSRPLAVLVNPAASGGRAIKALPAVQGELERLGIEFRVVHT
                     TSAANARSEAATSAERGEAVAGIGGDGLIGTLAGALCGTGVPLVVVPGGRGNDFARVL
                     GIPTDPAAAARLYVEGSERLLDVAEANDATFLGIASLGVDSDANRIANEAKLVKGNLV
                     YLYAAVRALIQWRDATFEVTVDGERHSFVGASVAVGNSKAYGGGMYALPHAELDDGLL
                     DVLVSTEKRKLGLMRLTLRAFTGSHVDDPTLKFLRGREVEVSADRPFDVYADGDPVAE
                     LPVRIRVAERVLRVMVPAR"
     gene            11229..11660
                     /locus_tag="VF517_12345"
     CDS             11229..11660
                     /locus_tag="VF517_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053774.1"
                     /translation="MERPGGGGRSHRPDRRAADIEYLTECGVRLVISTMTTRHGIAEY
                     EAAGLEWHHVPVASCAVGADALEELLPLLRRELRRSGAVAIHGNRHTDFVAAVAAAHL
                     YDHRGADPAASLAAAVAAGLTVTPAAAALLGVDLAEGTPDA"
     gene            complement(11661..11945)
                     /locus_tag="VF517_12350"
     CDS             complement(11661..11945)
                     /locus_tag="VF517_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053775.1"
                     /translation="MARKTHHKHSSTKGYRRLLDSLAAIEGVHRVATGRVTPRMGSGR
                     PVAAISKVRRVDSGLSITINTDGAMIDAYVITDRPEDVEAAMAERGWTVG"
     gene            complement(11945..12742)
                     /locus_tag="VF517_12355"
     CDS             complement(11945..12742)
                     /locus_tag="VF517_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX8053776.1"
                     /translation="MPLTPFIARYEQVILDLDGCVYVGGEAIDGAPEAVAALRDAGKR
                     IAFVTNDPRSSTEDYVRRLWGLGVQASLADVVTVGGAVQHVLAERYGGRTVFAIGTEA
                     LRRHVADSGVRVLNGTDLATRADIVVVGGTEDLVYEDLRVAALALRRGAEFLVTGRDP
                     TYPMPDGLWPGTGAIVAALEYSTERTAAVIGKPEPQLFLTALDRLGEGSTLAIGDRLD
                     TDVAAAGKVRLDAALVLSGGTTAAEAAAAPEPRPVRVAADLRELVLG"
     gene            12769..13473
                     /locus_tag="VF517_12360"
     CDS             12769..13473
                     /locus_tag="VF517_12360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8053777.1"
                     /translation="MRRALVIVSICLAAALGACGDDDKDEGAQDTSATTQAAPEPTQA
                     EPPAANAGGCKQVDEPTSAQRKAKEPKDELDAGKTYSLTFATNCGDFTIELDQKSAPK
                     TAASLVSLAEAKFYDGTAFHRIVPGFVIQGGDPTATGSGGPGYSTVDKPPADARYTKG
                     VVAMAKTQQEPPGTSGSQFFVVVGADVGLPPEYAVVGKVSKGMDTVERIGQQGDPATE
                     QPLQAVVIEKVTVKES"
     gene            13473..14225
                     /locus_tag="VF517_12365"
     CDS             13473..14225
                     /locus_tag="VF517_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8053778.1"
                     /translation="MVRDVNQADFEQNVIERSVEVPVVVDFWAEWCGPCRRLGPALEQ
                     AAKAREGQVDLAKVDVDGNQGLAQAFQVQGIPAVKAFRDRKVVNEFTGALPPAQVERF
                     FDELVPSEAEQQAEAALESGSEDGLRAALAADPRNAAVGRALGRLLLARDAVDDALEV
                     LAPHEHDFTAAGLAARARLSRNGAGLADAFDAWDRDDRAAALESLQTAFAAAAEDERE
                     DIRKVMVAIFTELGPDDPLAREHRRRLAAAMY"
     gene            complement(14264..14875)
                     /locus_tag="VF517_12370"
     CDS             complement(14264..14875)
                     /locus_tag="VF517_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053779.1"
                     /translation="MAKQRQQQKMVMQAGTLGAIQKLEPRSDEELIRETKHRAAARAI
                     LGARAAERYDAKQARTYFNEALAGTPPRERPALRQMMKASLALAERRPDDLKEAMESM
                     GQTPPSNRQLMVLRLMSLVSPPPGASALARARGFAVLIAIVVVLVLIGFGLAKLVALP
                     FGGVGTIGGILLGLVIVVAILGVLTLLGRRRQAKAQAAARAGG"
     gene            complement(14898..18419)
                     /locus_tag="VF517_12375"
     CDS             complement(14898..18419)
                     /locus_tag="VF517_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX8053780.1"
                     /translation="MKRGWRSGGMLAAWVCALLVLAVPAVAGPVATDENSYKQLGRVF
                     PDPLAGCQPGAGVCSPFAQGNSPATQFIGYQEFVNGMQFLSSKPEWRRYLEVWPLDGK
                     LGEGSGSKPGNEMFPGNDLGRLEFKPSSRYQSAGLPTTTQSRQKADLFVARVTDETVP
                     DKGKKRFALSLSIHGIERAGAEGGIRAIEDLVTAATTGAGGKRLLPKEAGESATFGET
                     LRNVIIYFTLPNPDGWRRGSVQEGGVFFQRYNGNGMDLNRDWPDIGFSHRFYTANSEP
                     ETRAFYNFYRGIEANEGQFTAGDDLHGQPFADALSYTMMPHGRHDWPKDARLRNAAVG
                     INHATNAMVSWSPIVQPNDQPQGGEIGCAPDVLGTPCSKIYGQTWGTVYDTINYTTAG
                     TLGDWFDSTEGLNAEGIDNEMSFSHLDKNITFDPHTEQMHVDGNKALIWSHVTSMLNP
                     PPDAKFDAPGRKAYVPNVRLKRAEKEAPKIPPGTVPQDDIPSAPSPGGTRTFEVKGGK
                     QPDGKDIYNGGMRIQATAANAQGIGPGVTTLTVQCRHCDAHTERKDAAGDDWVTVGED
                     FNQAPTYAQAGVTVSVNQPQAFYTDKDGKVAKVEWRAVVSTEGGPAPGIGIEFFSELA
                     SDDGNTGGDPPPRLAAYDVANTDVFRDLNPDMAPGVAGFDALDPRAVIAGQQSLTGLD
                     SLVLADDGLPGYKKQQQEGPVGTPPANRDIASSPTAPGGYGLAVDERAPGTYTKIDFE
                     LTPANPAGGVNIRIDWADETNDFDMYLYREAADGSDVLVASSTGTQLETKFEEINVED
                     PLPNGKYHLYVDNFAAPDFNWKGKMTFKQLFVKNEPTSFSEDEKNQWFAKVREWVAAG
                     GNLVLTDGALTALPDLVPGKFKSEDVEETTVYAGQVSFGKSSSESTLKDPLAQNPITL
                     QQPGARLNSGNRRQTYEPTPLGFAIQQDVRGNDDTSFAEQWDIDRKAWEAAGGRTAGT
                     SVDSGARDAAPVYDRVALGELKVGSGQIRVAGGLLPQPSTKYNHPLGIEPYSLTYSGY
                     ILLRNLLEVPQILSNPTIGGRFLISGRAVKMRKNAAAVRVSCRKPFVCKGTLTLQTRV
                     RERVKGKVKKKTVTLGKVKFNIGNKARNRVLQVPIRKSSRKYVLRTRRVNVLATAPIA
                     FADGKRGTARNDFWFYRPTSRALRRGR"
     gene            complement(18454..22008)
                     /locus_tag="VF517_12380"
     CDS             complement(18454..22008)
                     /locus_tag="VF517_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX8053781.1"
                     /translation="MATLRGYGRIRGVGAAAVGAVWLCALFLAGPAAATPVATNEGAY
                     AALGRVFPDPLAGCQAAGTSPCSPNAQGNVPATQFIGIDEFMDAIRYMNSKGDWQRYM
                     DIVALDGKLGDGAGSTTKDVDPNNNVPLEFKPDAKYVSAGLPTTELGRRKSDLMAIRV
                     TDENVPDKGKKRYALSLSIHGIERAGAEGGIRAMEDLVTAFTKQRADQPIAPKAVQPN
                     APTFADVLRKSIIYFTLPNPDGWRRGSVSSGGVFFQRYNGNGVDPNRDWPDIGFSYRG
                     YSAISEPETQAWIDFYTQVNGKSDPRFAAGDDLHGQPFADALSYTLLPHGRHSYAKNL
                     RIQDAAIAIHNGTYDAIKWSPIVVPDDSPRGGATETCEDTTLGTACGQIYAQTWGTVY
                     DTINYTTTGALGDWYDSSVGLNADGIDNEMSFSHLDKNINFDPHTEQLHVDGNKALIY
                     AHLTRILSPPPAAVFDADGRKAYVPNKRLRRDEKSNQPGPPAGSVAQFTYDSGIDPTP
                     GGGEFPFTVRQGQQPAGSGDDAGKNIFNGGMRIEVTDSNVQGVGSGQVTLQVQCKNCD
                     EHPGVKDADGFVTVAEDYNQSFLYAQSGIVATVNRPQAFGRDGKPVEWRAVIDGPTAA
                     ARVQVHFTQGPASASGETGGGIPPVLRKYDVANTDFFKDLNEFMPGTDEDMRAIDPAK
                     VIAGTQSLSGLDTIALADDALPGYTGDYGGVVSHPTGGPTPNQTFASAASAPGGGQGA
                     PRNPPAGTYEDREFTIGPNDSNKSAKIQMTWTNTAMDWDMYVFRKTASGGLASEGSSA
                     QGAPQTSETVIIPDPSPGTYVVRMINFAAPDPTFTLKVEFTAPPPAGQAPPSDFTGEQ
                     KDKWLDALRKFVEGGGNLVLTDGALRALPDLVPGGKIPGSKVQRQRVYVGQSAFAFNP
                     NGPSTAKDVLLRDTQPPGARNNTGFRRQMFEPTPLGFAIQTEDGGDASFARQYAVDKE
                     TWEKAGGRSVAASADAGARDAGPIFDQVTIGELKLGSGQIRIAGALLPQPSEEYDHEW
                     GIEPYAVTYTGYTVARNLLDQPNRGAGGGVGTIGGRFLISGRAVKMSRKGDAGVRVSC
                     RTPLQCNGKLTLYVKRAVRVKGKRRVRAVKIGAAKFNIRTKTRNTVLRVRLTKSGRKI
                     IRERRRTRVIAKAPVKFRDGLTGTARKTFRLYRPSRRR"
     gene            22140..22448
                     /locus_tag="VF517_12385"
     CDS             22140..22448
                     /locus_tag="VF517_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8053782.1"
                     /translation="MRTLHHPRVDELELPEVLHALSDPVRLEIVRLLADETERPCSSV
                     EAKVAKSTLSHHFKVLREAGITWTRANGTHRLVSLRTEDLEERFPGLISSVLNAAKAA
                     "
     gene            22644..23783
                     /locus_tag="VF517_12390"
     CDS             22644..23783
                     /locus_tag="VF517_12390"
                     /inference="COORDINATES: protein motif:HMM:NF016427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8053783.1"
                     /translation="MPAVTKDNPQHAARKRHADGGSAARKRGGDAAAEKPARKTTTRK
                     RGENSQDEPARKKTETVTKKNSKTTAAETKKETPTRRRRFAREDDGMAGKTAAEKAHA
                     QADDVTTDSLQLFFNEARKYPLLTAAEEIELAQRIEKGDVEAKDRMINSNLRLVVSVA
                     RKYQGHGLTLGDLVQEGMLGLIRATEKFDWRRGFKFSTYATLWIRQAIQRGLENSSRT
                     IRLPVHIAQRERKINRTERELATKLGREPTEEELAEAVELPIDQVIEIRKAARPLTSL
                     DQPVGDDGETAFGDLLASNAPNPEDEVVQDAGTSAVQRAVDALPERERDVVELRFGLG
                     GREPTPLRETGRRLGISAERVRQIEEDALERLAESGDLAALRDAA"
     gene            23862..24323
                     /locus_tag="VF517_12395"
     CDS             23862..24323
                     /locus_tag="VF517_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8053784.1"
                     /translation="MTLPPFQAFLDAHAEAIHRFLVALVGPQEADDALQETLIAALRA
                     YPRLRPGSNLRAWGLSIARNKAIDSTRARARRPVPVAASVEVAVPAGAEPDGEVWAAV
                     RVLPAKQRTAIALRYAADLRHREVALAMGVSEEAARRSVSDGLKRLREVLG"
     gene            24320..24913
                     /locus_tag="VF517_12400"
     CDS             24320..24913
                     /locus_tag="VF517_12400"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX8053785.1"
                     /translation="MKERLTIRSPLPDGALARFAERAGEEGLVDVAYATLDTPVGELL
                     VASTRRGVVRISYLDWFPAEATIADLARRISPRVLEAPARLDGVRRELDEYFAGERRS
                     FDVRVDRSLLGPFARKVLGRTARIPYGEVSTYAAMAAAAGSPRASRAAGNALASNPIP
                     IVIPCHRVLRSGGGMGGYTGGLERKAQLLELEGWSAV"
     gene            24936..>25570
                     /locus_tag="VF517_12405"
     CDS             24936..>25570
                     /locus_tag="VF517_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053786.1"
                     /translation="MPRRLTVIIALAAVAAVLGGFVLLTGGKDTVPLSTVANAAEATN
                     SAGGFKLSIDGSFEIPQVNRTLPMKGSGALDPKARRGRLVFDSAGALAPTGGASGRIE
                     QVFEGDVIYMTMPAIAQQLGGKKAWLRVDIKEAGRALGVDPSQFSQLGGNDPRQMLDQ
                     IRSVSGEVEKLGAEKVRGVDTTHYRASVDLRRYPDRLPEAQREQARVAVERL"
BASE COUNT         3515 a         9156 c         9176 g         3723 t
ORIGIN      
        1 cctgccgcgt ggcgatgggg cgggccgcca cggcctccaa ggattttcct ggcgggccgg
       61 aggatgggac cggcaccgtc agctcgggtg ggcgcgagtg ggagttccag catgtctcga
      121 tcggcaaccc gcagtgcgcg atcgaggtgg gcgacgagct gaccgagctc gacctgagcg
      181 tgctcggccc gccgatcgag ggcgacaccg agctcttccc gaacaggacc aacgtgtcgt
      241 tctggcgccg cgacggcgac gccgcgatca cggcgcggat cttcgagcgc ggcgtggggg
      301 agacgatgtc gtcgggcacg ggggcgagcg gcgcggccgt cgcggccgtg ctgcgcgggg
      361 tggccagccc cgtgaccgtc cacctcgacg gcggcgagct cgaggtcgag gtgggggagg
      421 acctgaacgt gaacctgacc ggctgggccg tgcccgtgta cgagggacgg ctctccgaga
      481 acttcgtgga ggagctgcgt gaagccgagt agccgcctgg agcggatccc gccgtacctg
      541 ttcgccgagc tcgagcggaa gatcgccgca aagaaggagc agggggtgga cgtgatcagc
      601 ctcgggatcg gcgaccccga cacgccgacg ccggcgctcg tggtcgacgc gctcgccgcc
      661 ggcggggccg accccgccac ccaccagtac ccgtcgaatc gcgggcgggc ggacttccgc
      721 gaggccgtcg cgcgcttcta cgagcgccgc ttcggcgtga cgatcgaccc ggccaccgag
      781 gtgatccccg cgctcggcgc caaggagtgc gtgttcaacc tgaacctcgc gttcctcgac
      841 cccgacagcg tcgcgctcgc gagcgacccg ggttacccgg tctacaccgg cgggccgctg
      901 ctggccggcg ccgaggccgt gctgctgccg ctgcgcgccg acgccgggtt cgcgcccgac
      961 ttcgactcga tctccgccga ggactgggcg cgcgcccggc tgatgttcct caactacccg
     1021 aacaacccga ccggcgcggt gctgccggac ggcgtgttcg agcgggcgat cgagctggcc
     1081 cacgagcacg acctgctcgt ggtgcacgac gcctcctaca ccgagacgac cttcgacggc
     1141 taccgcgcgc cgagcttcct cgagtgggac ggcgcgcgcg aggtcggcgt cgaggtcttc
     1201 tcgctctcca agggctacaa catgaccggc tggcggacgg ccgccatcgc cggtaacgcg
     1261 gaggcgatcg agcactactg gcggctgaag tcgaacatcg actcgggcat gttcgaggcg
     1321 gtgcagctcg cggccatcgc ggcgctcgac gagggcgacg cgaccgcgcg ggggatgagc
     1381 gaggtgtacg cgcgccggcg cgacctcgtc tgcgacgcgc tcgcccagat cggcgtggac
     1441 gtgacgccgc cgaagggcac gatctacatc tgggcgccgg tgcccgaggg gcacacctcc
     1501 gcgtcgtact gcgagctggt cctcgaggag tcgggcgtgg tgctctcgcc gggcggggcg
     1561 tacgggccca acggcgaggg tttcttccgg atctcgctca cggtgcccga cgagcggctg
     1621 ctcgaggcgg tcgagcgcct gcggtcgagc ctcgggggct gagcatccgc cgcctggtcg
     1681 cgatcctcgt cctggcgctc gtcgcctgcg ccgctcccgc gcaggccgcg aagtggccga
     1741 gcaagcgcct ggcgctcggg gtcaccgact cgccgggcgg cgccaaggcc ctacgcaaga
     1801 gcgtccagct cacgttccgc taccagtacc tcgccgccgg tgtgaacacg gggagcggct
     1861 gggcgacctg gaacgagggc ggcacgttcg tcgacaacta cgtgcgcgag tccgcggcag
     1921 cgcgcgtggt gcccgtcttc acctactaca tgatccgcca gtcgctgccc ggccgcgacg
     1981 acggcgacga gccgcgggcg gtgctctcga acctgcgcaa cgcgtcgacc atgcgggcgt
     2041 atctggacaa cctgcggctc ttcttcgagc gcgcggcgcg gttcccgaag acgcggatcg
     2101 tgctgcacgt cgagccggac atgtggggct acgggcacca ggtggcgaag aacgacgacg
     2161 cggcgagcgt gccggtggcg gtggtgggcg acctcggcgg gctcgcgcgc gaggtggtgc
     2221 ggctgcgcga cgagatcgcg ccgaacgtgg tgctcggctg gcacgtgagc ggatggggca
     2281 cggggatcga cctgcaggtc aacgacccct cgaccaagca atcgtccgcg ctcggggcgc
     2341 gcgccgcccg cttctaccgc tcgctcggtg cgcgcttcga catcacgttc accgactggt
     2401 cggaccgcga cgcgggcttc aaggagagga tcctcggccg cacgcacgcc gacgcctggt
     2461 tcacgccggg tgactacacg cgctggcggt acttcatgtc cgcctactcg cgcaagacgg
     2521 gccagcggat cgtggtctgg cagatcccgc tcggcaacac cgccctggac gactcgtggg
     2581 gccgctaccg cgaccgccac gtgcagtggc tgatcggcgc cggctacggg agccggctga
     2641 aggccatgcg ttcggcgggc gtcagcgcgc tgctgttcgg cggcggtgcc gacgggacga
     2701 ccagcgcgaa gaccgacggc ggctacttcc gcaagcgcgc ccggacgtac tacaggaccg
     2761 gaccgctgcg gctgccgtag ccgcgagtcc atttgtacgt cctatagcac tacgaaatgg
     2821 actctcaccc ggaggcggcg agcgtctggc gcaggccggt ctccaggtcg cgcggcgcgt
     2881 acccgagccg cgtgcgcgcc ttgtcgtccc tggcccagta ggtcacgccg tccgaggccg
     2941 agatcagctc gcgcatgttc ggagccatgc ccatcagctt ccccaccagc ggccccgcgg
     3001 gcgcgatcgc cttcatcacc cccgtgggga ggttccgctt cggcggcttc ttcccgtcga
     3061 tctgcgcgac cttctccacg atcccgcgca tcgtcgtgat ctcgccgccg agcacgtacg
     3121 actcgcccgg ctcgccgtgc tcgtaggcga ggatgcagcc ctccgccacg tcgtcgacgt
     3181 ggaccatgtt gagcccagtc tgggggaacg gcagcatcgg cagcttgccc gcccggaact
     3241 gctcgatcat gttcccgatc tccgagtggt cgtcgggccc gtacacgccg cccggctgga
     3301 cgatcacgac cggcccgccc ttgccgatcc ggtcgagcgc gacctgatgc gactcgaact
     3361 tcgtctggtc gtagtaggag aggaagccgt tcggcgggcg ctcgtaggac tcgtccacga
     3421 tttcgccgcc ggtgttcccg aacacgttga tggtgctgag gtagacgatc ttcggcacgg
     3481 cggcctggat cgcggcgtcg agcacgcgct cggtaccgcg cacgttcgtc tcgtacatct
     3541 ccgcgtgctg cgacttcggg atcccgacct tgtagatcgc ggccccgtgg aagcaggcgt
     3601 cggcgccctc ggcgccgcgg cggatcgcct cgacgttcgt caggtcgccc tccaccagct
     3661 cgacgcccgc tcgcttcagg tcggcggcct tgtcggggtt gcgcacgagg gccacgacgt
     3721 cgtggccgcg ctcgcgcagc ttgctcacca gccgctggcc gatgaagccg gtcccgcccg
     3781 tcacgaaggc cctcacgtgg agcaacctat cgcgcctaga ctgcccgtgt gaatcgcgga
     3841 cgagacggac gccgctcgga aacccaatat ggcgacggaa ttcgcaatcc gcgggcccgc
     3901 cagcgggcgt tctgcatcgc cgtcgcgccg gacgaacccg acctctccga gctgcgcgag
     3961 ctgctgctga ccgctggcgt cgcggtggcc ggcgagatgg tccagcgccg cgagcggccg
     4021 gacccggacc gctacttcgg caagggcaag cgcgccgagc tgaagcgcgc ggtgcaggag
     4081 gcggacgcga acctagtcgc ctgcgacgac gagcttctcc cgcgccagga gcgcaacctc
     4141 gaggaggagc tcggagtgcc ggtgatcgac cgcaccgccg tgatcctcga catcttcgcc
     4201 gaccacgccg tgtccgccga gggcaagctc caggtcgagc tggcgcagct cgagtacaac
     4261 ctcgcgcgca tgcgcgggct ctggacccac ctcgaacggc tgggggccgg gcgcgtcgac
     4321 ggcggcatcg gcacgagggg cccgggcgag tcgcagatcg agaccgaccg gcggctggcc
     4381 cgcgaccgga tcaccgcgat ccgccgccgc ctcgaccgca cccgcgagcg ccgtgacacg
     4441 cagcgcggcg agcgcgagcg cgcgcacctg ccgaccgtgg cgctggccgg ctacaccaac
     4501 gccgggaagt ccaccctcct gaacggcctc accggcgccg aggcgcaggt ccgcgaccgg
     4561 cttttccaca cgctcgaccc gctcacgcgc tcgttcgaga tcgccgggcg ccagtacctg
     4621 ctgaccgaca ccgtcgggtt catccgcaag ctcccgcatc agctcgtgga cgcattcgcg
     4681 tcgaccctcg aggagaccct gcgggccgat ctgatcgtgc acgtcgtgga cgcgtccgca
     4741 cccgaggacg aacagaccga gatgatcggc gcggtcgagg aggtcctcac cgagatcggc
     4801 gccggcgagc agcccacggt gctggcgctc aacaaggtcg acctgctcga cgaggagcgc
     4861 cggcgcgagc tgtcgttccg ctacccgcgc ggcgtgcagg tctcggccgt gaccggggac
     4921 gggctcgacg agctgcgcga cgccgtcgag gcccagttcc tgagcacgct gcgaccgatg
     4981 gagctgctgc tcccgtacgg cgagggcgga tcgctctcgg cgctgcacga cgccgcgggc
     5041 gaggtcgagc gcgaggacac cgtcgagggc gtgcgcgtga aggcgcgggt gcccgcgcgc
     5101 gtggcggcgc gcttcgagcg cttcgaggtg aacggcaacg gcgcctcgcc gaacggcggc
     5161 ggctccgtcg gcccgcgcga gtgacgctgc gcttcacgcg gctgcacgcc gaggcccggc
     5221 cccccgcgca ggcgcacgag ggcgacgcgg gccatgacct ccacgcggcc gagccggcca
     5281 cgctcggtcc gggcgagcgc gcctcggtgg ggaccggcat cgcggtcgcg atcccggagg
     5341 gctgcgccgg gctcgtgctg ccccgcagcg ggctggcggc gcgccacggc gtgacgctcg
     5401 tcaacgcgcc cggcctgatc gactcgggct accgcggcga ggtgcgggtg ctgctgctga
     5461 acaccgaccg cgacgagacg ttcgaggtct ccgccgggga ccgtatcgcc cagctcgtgc
     5521 tggtccgctt cgaggccggc caggccgagg aggtcgagtc gctggatgcg acggcccgcg
     5581 gcgccggcgg cttcggctcg acgggtcgct gaaacggcat ggagcccgag gacgtcgaac
     5641 tcctgtcggt cgtggatccc gaggcgtacc cgcttgtgag ttgagtcacg cgtgccttga
     5701 ccggtgcggg ggagaggcgt agcttccgcg ccatgtcgcg ccacacgagc tcctccactc
     5761 gcatcgcgcg ccgcccggcg ccgcacctgc gcgcctccga cgccgatcgc gaccgcgcgg
     5821 tggacatgct gcgcgaccac gccgcggcgg gccggctctc caccgaggag ctcgaggacc
     5881 gcaccgattc cgcgctggcg gccacgacgt tcggacagct cgacgcggtc ctgtcggacc
     5941 tgccgaggca gcgcgacccg cggcgcgccg agcgccgcca cgacctggca cgcaaggcgt
     6001 tcgccaccca cctgcgcacc tacgtgatgg tgatggtgct gctggtcgcg atctgggcgc
     6061 tcacgggcat gggctacttc tggcccgtct ggcccgcgct cggctgggga atcggcatcg
     6121 cgagccacgg tgcctacacg ccgcgcggcc ggcgcggcca cggccagccg cgcgcgatgg
     6181 cccggcccta gacgccgggg acctcatcgg cggccacgca cggcccgagg ccgtcgagct
     6241 gcctgcgctc gacgaccccg agccatacgt ctgagacccg cggctactgc gtgtctcgct
     6301 cgaacgtcac gacgggcggg accggcccgg tcacgacggt gtagcgcgtg ctgttgacgc
     6361 agaccgacac cggcgtctgg cccaccggac tcgtccggag cgccagcggc gggttgccga
     6421 gcacgagcac gtcgttcgtg cacggtgtga tgtcggacga ctgctggacg tcgacgacct
     6481 ggttcaccgc ggccttcacc ccgaggtgga agccggccgc cccctgcggg ctcgacaggc
     6541 gcgcgcagac gtgcacctcg ttgtcggccg gcttggcgtt gtagaggaac aggtcgcggt
     6601 cggcgaggtg cgcgttgacg ttctcggtcg cgggaccgta cgcccccgcc gagcacgacg
     6661 tcgagggctg cccggccggc ggcggcgtgg tgtccgcgat cggcccgggt gcggtgtccg
     6721 agttggccgt cacgtcgggc tcgttgaggc ccgccgacgc gttcaccagc acccggtgct
     6781 tgaccggtcc ggcctcgacg cagagccacg tctgcgaccc ggcaccgtag gcgtcgatgt
     6841 agaacggcgt ctccgcgacc gtgccctgct ggatcgggtg tgtggtgggc agcgtgttcc
     6901 ccacggtggt cgtgcacgcg cgcgaatcga cgtcggtcgt cacgcgggcg accggcgcca
     6961 cgtcggtggt cgcgttcacg tcgagccgcc cggccgtctc cggctggttc gggaccttga
     7021 tccggtagca gacccagttc gtgcttggat cgaccgggtc ggcctgtgtg ctgatcctga
     7081 agtacacgga gcggttcgcg aagaacccgt cgttcatcgt ggccgggttg gtgcacgtgt
     7141 tggacactcc cgcgacgttc gcggtgctca cgcacccggc cacgaagacc gccgccgcca
     7201 agagcgcacc gacaatcccg atgctgcgca tcatctttcc cccctgagtc tcgtcccgtc
     7261 cggccccccc ggacgagttc aagagtcatt gcacgctcag gattcgttga aacgcgcagg
     7321 tgccgaagat caccgctctg gggtaaggcg ccgcccgccg cctagacccc cgcggcccgc
     7381 cgcgcgctca tgtgcgcggc gtcctccatg gtgtcgtcga gcgggtcgag ctcgatgccc
     7441 agcgccaact cgatcaggcg atccgcgagc cagacgttct tcggcagcag cgggccgtgc
     7501 aggtacgtgc cgatcacgtt gcccttgtgc actccctcgt ggtcgtcgcg gccgttgttg
     7561 ccgtgaccgc tcagcacgcg gccgagcggc ttctcgcccg cgccgaggta ggtacgcccg
     7621 ccgtggttct cgaagccggc cacgacgcgc gtgccgccgc ccaggtcggc ctcgatcgcg
     7681 cagttgccga tcagccgctc gcccggctcg cgcaccgtcc gcaggtcggc gaggccgatg
     7741 ccgggcagct cctcgtcgcc catctcgtag ctcgacccca ggagctggta gcccccgcac
     7801 acggcgagca cgacggcgcc gcgatcggcc gcggcgtgca gggcgtcgcg cttggtcgcg
     7861 accatgtcgt gggccaccgc cgcctggtcg cggtcctgcc cgccgcccat atagaagagg
     7921 tcgtgcgcgg cgggatccac ggactcgccg acactcgacg ccgcaagctc gaagccgatg
     7981 ccgcgccact cgcagcgcgc gcgcagcaca gcgatgttgc cgcggtcggc gtagatgttc
     8041 atcagatcgg gatagagcga gcagaccctc agcgcgcgcg tcacggcagc ttctccagca
     8101 gaaccacggt gctgcccacg tagtcgcccg tggcgggcac gtggcggcgc gggagggtgc
     8161 gcatccggac gctcgcgcca aggtgctcga gttcctccgc gggagcgtcg tcgaggcgga
     8221 tgctcgccac gacctcctcg agccgaccct ccggcgagac cgactggcgg tggcggtcga
     8281 tcacggtggc gcccgcctcc gcgcgcaccg cgatcggcgt gctcgagaag acccagccgt
     8341 cctgctcgcg cacgtccggc actggcgggg ccgcgtcctc ggccgggatg ccctcgaacg
     8401 ggtccgccag cgcggcggcg aagcggcctc cgaccgtcag gtgctcgcgc acgctgccga
     8461 gcatgcgaag ccgcccggac gggccgccga ggagctggac cacctgcatg ggggcgatgg
     8521 cgagcgcgaa ctcgcgaccg agtgcgaacg atcgcgcgtc cgccacctcg gtgcgcactc
     8581 gcaacccgcg ctcgcgcgcg cgggcggcca gcgcgcgcag gaacgcgggc tcggagtcga
     8641 gggccgtcac gtcgtgcccc gcgcccgcga gctcgagcgc aacgcgtccg gtcccggcgc
     8701 cgaggtcgag gatcgggcca tccgccgcgg cggcaagctc gtgccagagc gggaggtcgg
     8761 ccgcgtaggc ggcgcactcc acgtcgtgcc agacgaccgg gtcggccacc ggagccgcct
     8821 tcacggacgc gcccccgccg ccggccgcct ccggtcgccc gagcggcggc cgcgcgtcac
     8881 tcgctccacc ttcccgccac cccgcggtcg gccaacagat cgcgcaggtc gagcagcgcc
     8941 gtgtaggtcg gcagggcgta caggggcgtg ccgccgtcgc ggtcggccac cgcggcgtcg
     9001 agcgagcgct cgaggtcgcg ctccaccgcg atcggcgcgt cgattcccgc gtacttcagc
     9061 cgcagcgcca tctcctccgc gcgcgtgccg gagcaggtga cgcggcggac ccgtccggcg
     9121 agcagctcga agtcggcgtc ccagatccag gagatatcgc ggccgtcggc gatccggtcg
     9181 ttcagcgcca gccagaggtc gatccggccg tcctcgaggg tcagcgtgcg cagcacctcg
     9241 ttggcgccga ccgggttctt cacgagcagg atcgagacat cgcgaccgga caccgacagg
     9301 cgctccacgc ggccgaacac ggccgacatc ccctcgaggc cctcgcgcac ccgctccagc
     9361 gacacgccga gttcgagcgc cgccgtggcc gccgccagcg cgttgtagac gttgtagagc
     9421 ccgggcagcg gaagccgcac gtcgaccgag ccgcccgagg tacgcagcgc gatatccgac
     9481 ccgtccatcc cgcgcagcgc cactcgcgtg gccaccacct cggggaccgg gcgggtgcgg
     9541 ccgcagttcg tgcagcgata gcggccgaga tgccccatgt agatcgcgtc gtagacgtac
     9601 gggtgcccgc agttgcggca gtgcttcgag tcggcggcgt gctggagctc gggcagcgcg
     9661 agcgtgtcgt cctcgacccc gaaataggtc acccgcgagt gctcgcgacc gaggtcggcc
     9721 acgagcgggt cgtccgcgtt cagcacgagc gcggtgcgat cgccgcgcgc cgcgacgagg
     9781 tccgcccagc ggtcggccag cagctcgagc tcgccgtagc ggtcgagctg gtcgcggaac
     9841 aggttgccga ggatcagcag gcccgggtcg agctgctcgg cgatgcgagc gagccacgcc
     9901 tcgtcgacct cgaacaggcc cagctcgccc ggctcgcgcc ccgcgtcgat gagggcggtc
     9961 gccacgcccc agtgcatgtt cgacccggcg cggttgcgga ccaccgtccg ctcgtcgcgc
    10021 tcgagcacgc cggcgagcat cgaggcggtg gtggtcttgc cgttggtcgc ggatatgagc
    10081 gtcacgccgc ccgcgatccg gcggccgagg tgcgcgatcg cccgggggtc gaggcggagc
    10141 agcacccgcc ccggcacggt cgtgccgccc cggccggcgc gccggatcgc ggcccgggca
    10201 aggcgggcga ccgccaccgc gaggcgcagc atcaccgtgc cggcaccatc acgcgcagca
    10261 cccgctcggc cacgcggatc cggacgggca gctccgcgac cgggtcaccg tcggcgtaga
    10321 cgtcgaacgg gcggtccgcg ctgacctcca cctcgcgccc gcgcaggaac ttcagggtgg
    10381 ggtcgtcgac atgcgagccc gtgaacgccc tcagcgtcag tcgcatcaga ccgagcttcc
    10441 gtttctcggt gcttacgagg acgtcgagca gcccgtcgtc cagttcggcg tgcgggagcg
    10501 cgtacatgcc gccgccgtag gccttcgagt tgcccaccgc cacggaagcg ccgacgaacg
    10561 agtgacgctc gccgtcgacc gtcacctcga acgtcgcgtc cctccactgg atcagggcgc
    10621 gcacggcggc gtataggtag acgaggttcc ccttgacgag cttggcctcg ttcgcgatcc
    10681 ggttcgcgtc ggagtccacg cccaggctcg cgatccccag gaaggtcgcg tcgttcgcct
    10741 cggccacgtc gagcagccgc tccgagccct cgacgtagag gcgcgccgcc gccgcgggat
    10801 cggtcgggat gccaagcacg cgcgcgaagt cgttgccgcg gccgccgggc accaccacga
    10861 gcggcacgcc ggttccgcag agcgcgcccg ccagggtgcc gatcagcccg tcgccgccga
    10921 tgcccgccac cgcctcaccg cgctccgcgc tcgtcgcagc ttccgagcgc gcgttggcgg
    10981 cgctggtcgt gtgcacgacg cggaactcga tcccgagccg ctccagctcg ccctgcacgg
    11041 ccggcagcgc cttgatggcg cggccaccgg acgccgcggg atttacgagc accgcgagcg
    11101 ggcggctggg gtcggccatc ggggcgagag tctaaaaggg gggcgaacct ccgcggcggc
    11161 gcccttaggg aggcatgaca catcgaccga agcgaatctg gtgggcgcgc ccgcgcaagc
    11221 tgtgcgcgat ggagcgaccg ggcggcggtg ggcgcagcca ccggcccgat cgccgcgcgg
    11281 ccgacatcga gtacctcacc gagtgcggcg tgcggctcgt gatcagcacg atgaccacgc
    11341 gccacggcat cgccgagtac gaggccgccg ggctcgagtg gcaccacgtg ccggtggcat
    11401 cctgcgccgt gggggccgac gcgctcgagg agctcctacc gctgctgcgg cgcgagctgc
    11461 gccgctccgg cgccgtcgcg atccacggca accgccacac cgacttcgtg gcggcggtcg
    11521 ccgcggccca cctgtacgac caccgggggg ccgatcccgc cgccagcctg gccgccgccg
    11581 tggcggcggg cctgacggtc acgcccgccg cggccgcgct gctcggcgtg gacctcgcgg
    11641 agggaacgcc ggacgcgtga tcagcccacg gtccagccgc gctcggccat cgccgcctcg
    11701 acgtcctccg ggcggtcggt gatcacgtag gcgtcgatca tcgccccgtc ggtgttgatc
    11761 gtgatcgaga ggcccgagtc gacgcggcgg accttcgaga tggcggccac gggccggccc
    11821 gagcccatcc gcggcgtcac gcggccggtc gcaacccggt ggacgccctc gatcgcggcc
    11881 agcgagtcga gcagccgccg gtagcccttc gtcgacgagt gcttgtggtg ggtcttgcgg
    11941 gccatcagcc gagcaccagc tcgcgcaggt ccgcggccac gcgcaccggc cgcggctcgg
    12001 gggccgccgc cgcctcggcc gccgtggtcc cgccgctcag caccagcgcc gcgtcgaggc
    12061 gcaccttccc ggcggcggcg acatcggtgt cgagccgatc gccgatcgcg agcgtgctgc
    12121 cctcgccgag ccggtcgagc gccgtcagga agagctgagg ctccggcttc ccgatcacgg
    12181 cggccgtacg ctcggtcgag tactcgagcg ccgcgacgat cgctccggtg ccgggccaga
    12241 gcccgtccgg catcgggtag gtcggatcgc gcccggtgac gaggaactcg gccccacgcc
    12301 gcagcgcgag cgccgccacg cgcaggtcct cgtacacgag gtcctcggtg ccgcccacga
    12361 ccacgatgtc ggcgcgcgtg gcgaggtcgg tcccgttcag cacgcgcacg cccgagtccg
    12421 ccacatggcg ccgcagcgcc tcggtcccga tcgcgaagac cgtccgcccg ccgtagcgct
    12481 cggccagcac gtgctgcacc gcgccaccca ccgtcacgac atccgccagc gacgcctgga
    12541 cgcccaggcc ccagagccgc cgcacgtagt cctcggtcga gctgcgtgga tcgttcgtca
    12601 cgaatgcgat ccgcttgccg gcgtcgcgca gcgcggcgac ggcctccggc gctccgtcga
    12661 tcgcctcgcc tccgacgtag acgcacccgt cgaggtccag gatcacctgc tcgtaccgcg
    12721 cgatgaaggg cgtgaggggc atgagccctt gaagctaccc tctgcgcggt gcgacgagcc
    12781 ctcgtcatcg tctcgatttg cctcgcggcc gccctcggcg cctgcggcga cgacgacaag
    12841 gacgagggcg cccaggacac ctccgccacc acccaggcgg cgcccgagcc gacgcaggcc
    12901 gagccccccg ccgcgaacgc cggtggctgc aagcaggtcg atgagcccac ctccgcgcag
    12961 cgcaaggcga aagagccgaa ggacgagctc gacgccggca agacctacag cctcacgttc
    13021 gccaccaact gcggcgactt cacgatcgag ctcgaccaga agtccgcccc caagaccgcg
    13081 gcgtcgctcg tctcgctcgc cgaggcgaag ttctacgacg gcaccgcctt tcaccggatc
    13141 gtgcccgggt tcgtgatcca gggcggcgac ccgaccgcca ccggcagcgg cggccccggc
    13201 tactcgaccg tggacaagcc gcccgccgac gcccgctaca ccaagggcgt ggtggccatg
    13261 gccaagaccc agcaggagcc gcccggcacg tcgggcagcc agttcttcgt cgtggtcggc
    13321 gccgatgtcg gcctcccgcc cgagtacgcc gtcgtcggca aggtcagcaa gggcatggac
    13381 acggtcgagc gcatcgggca gcagggcgat cccgccaccg agcagccgct ccaggcggtg
    13441 gtcatcgaga aggtgaccgt caaggagtcc tgatggtccg cgacgtcaac caggccgact
    13501 tcgaacagaa cgtgatcgag cggtcggtcg aggtgcccgt ggtggtcgac ttctgggccg
    13561 agtggtgcgg cccgtgccgg cgtctcggcc cggcgctcga gcaggcggcc aaggcccgcg
    13621 agggccaggt cgatctcgcg aaggtggacg tcgacggcaa ccagggcctt gcccaggcat
    13681 tccaggtcca gggcatcccg gccgtcaagg cattcaggga ccgcaaggtc gtcaacgagt
    13741 tcaccggcgc gctgccgccc gcccaggtgg agcgcttctt cgacgagctg gtcccgtccg
    13801 aggccgagca gcaggccgag gccgcgctcg agtccggctc cgaggacggc ctgcgcgccg
    13861 cgctggccgc cgacccgcgc aacgcggccg tcggccgggc gctcggccgg ctgctcctag
    13921 cgcgagacgc cgtggacgac gcgctcgagg tgctagcccc gcacgagcac gacttcaccg
    13981 ccgccggctt ggccgcccgc gcccgcctct cgcgcaacgg cgccgggctc gccgacgcct
    14041 tcgacgcctg ggaccgcgac gaccgcgccg ccgccctcga gtccctccag accgcgttcg
    14101 ccgccgccgc ggaggacgag cgcgaggaca ttcgcaaggt gatggtcgcg atcttcaccg
    14161 agctcggccc ggacgacccg ctcgcacgcg agcaccgccg ccgcctcgcc gcggcgatgt
    14221 actagggccg caggcgcgga gcgtcagccg caggcgcgga gcgtcagccg ccggcgcggg
    14281 ccgcggcctg cgccttggcc tggcgccgcc tccccagcag cgtgagcacg ccgaggatgg
    14341 ccaccacgat cacgaggccg agcaggatcc cgccgatcgt cccgacgccg ccgaacggca
    14401 gggccaccag cttggccagc ccgaagccga tcagaacgag cacgacgacg atcgcgatca
    14461 gcacggcgaa cccgcgcgca cgcgcgagcg cgctggcgcc cggcggaggc gagaccaggc
    14521 tcatcagccg gagcaccatg agctggcggt tcgacggcgg agtctggccc atcgactcca
    14581 tcgcctcctt gaggtcgtcc gggcggcgct cggcgagcgc cagcgacgcc ttcatcatct
    14641 gccgcagcgc cggccgctcg cgcgggggcg tgcccgccag cgcctcgttg aagtaggtcc
    14701 gcgcctgctt ggcgtcgtag cgctcggcgg cccgcgcccc gaggatggcc cgcgcggcgg
    14761 cgcggtgctt ggtctcgcga atcagctcct cgtcggagcg cggctcgagc ttctggatcg
    14821 ccccgagcgt ccccgcctgc atgaccatct tctgctgctg ccgctgctta gccacggcgg
    14881 gggagtgtag ggcggggcta gcgcccgcgg cgtagagcgc gcgaggtggg gcggtagaac
    14941 cagaagtcgt tgcgcgcggt gccgcgcttg ccgtcggcga acgcgatcgg cgccgtggcc
    15001 agcacgttca cgcggcgggt gcgcagcacg tacttgcgcg aggacttgcg gatcggcacc
    15061 tggaggaccc ggttgcgggc cttgttgccg atgttgaact tgaccttgcc gagcgtgacg
    15121 gtcttcttct tcaccttgcc cttgacgcgc tcgcggaccc gggtctgaag cgtcagcgtg
    15181 cccttgcaga cgaacggctt gcggcaggag acgcgcacgg cggccgcgtt cttgcgcatc
    15241 ttcaccgcgc ggccggagat caggaagcgc ccgccgatcg tcgggttcga caggatctgc
    15301 ggcacctcga gcaggttgcg gagcaggatg tagcccgagt aggtgagcga gtacggctcg
    15361 atgccgagcg ggtggttgta cttggtcgag ggctggggga ggaggccgcc ggccacgcgg
    15421 atctggccgc tgcccacctt gagctcgccg agagcgacgc ggtcgtacac cggggcggcg
    15481 tcgcgcgccc ccgagtcgac cgacgtgccg gccgtgcggc cgcccgccgc ctcccaggcc
    15541 ttgcggtcga tgtcccactg ctcggcgaac gaggtgtcgt cgttgccgcg cacgtcctgc
    15601 tggatggcga agccgagcgg ggtgggctcg taggtctgac ggcggttccc gctgttgagc
    15661 cgcgcgcccg gctgctggag cgtgatcggg ttctgcgcca gcgggtcctt cagggtgctc
    15721 tcgctcgacg acttgccgaa cgagacctgc ccggcgtaca cggtcgtctc ctcgacgtcc
    15781 tcgctcttga acttgccggg gacgaggtcg ggcagcgccg tcagcgcgcc gtcggtgagc
    15841 acgaggttgc cacccgccgc gacccactcg cgcaccttcg cgaaccactg gttcttctcg
    15901 tcctcgctga agctcgtcgg ctcgttcttc acgaagagct gcttgaacgt catcttgccc
    15961 ttccagttga agtcgggcgc ggcgaagttg tccacgtaca ggtggtactt gccgttcggc
    16021 agcggatcct cgacgttgat ctcctcgaac ttcgtctcga gctgggtgcc ggtcgaggag
    16081 gcgacgagca cgtcgctgcc gtcggcggcc tcgcggtaga ggtacatgtc gaagtcgttc
    16141 gtctcgtcgg cccagtcgat ccggatgttc accccgccgg ccgggttcgc gggcgtcagc
    16201 tcgaagtcga tcttcgtgta ggtcccgggc gcgcgctcgt cgacggccag cccgtagccg
    16261 ccgggggcgg tcgggctcga ggcgatgtcg cggttcgcgg gcggcgtgcc gaccggtccc
    16321 tcctgctgct gcttcttgta gccggggagg ccgtcgtcgg ccagcacgag ggagtcgagc
    16381 cccgtgaggg actgctgccc cgcgatcacg gccctcgggt cgagggcgtc gaagcctgcg
    16441 acgccgggtg ccatgtcggg gttgaggtcg cggaagacgt ccgtgttggc cacgtcgtag
    16501 gcggcaagcc gcggcggcgg atcgccgccc gtgttgccgt cgtccgaggc gagctccgag
    16561 aagaactcga tgccgatgcc gggggcgggg ccgccctcgg tgctcacgac cgcgcgccac
    16621 tccaccttgg ccaccttgcc gtccttgtcg gtgtagaacg cctgcggctg gttgacgctc
    16681 acggtcacgc cggcctgcgc gtaggtgggg gcctggttga agtcctcgcc gacggtgacc
    16741 cagtcgtccc cggcggcgtc cttgcgctcg gtgtgcgcgt cgcagtgcct gcactggacg
    16801 gtgagcgtgg tgacgcccgg cccgatcccc tgcgcgttgg cggcggtcgc ctggatgcgc
    16861 atgccgccgt tgtagatgtc cttgccgtcg ggctgctttc cgcccttgac ctcgaaggtg
    16921 cgggtgccgc cgggggaggg cgcgctcggg atgtcgtcct gcggcaccgt gcccggcggg
    16981 atcttcggcg cctccttctc ggcgcgcttg agccgcacat tcggcacgta ggccttccgc
    17041 ccgggcgcgt cgaacttcgc gtcgggcggc gggttgagca tcgacgtgac gtgcgaccag
    17101 atcagcgcct tgttgccgtc cacgtgcatc tgctcggtgt gcgggtcgaa ggtgatgttc
    17161 ttgtcgaggt gcgagaagga catctcgttg tcgatgccct ccgcgttgag cccctcggtc
    17221 gagtcgaacc agtcgccgag cgtgcccgcc gtcgtgtagt tgatcgtgtc gtagacggtg
    17281 ccccaggtct ggccgtagat cttcgagcac ggcgttccga ggacgtcggg cgcacagccg
    17341 atctcgccgc cctgcggctg gtcgttgggc tgcacgatcg gcgaccagct caccatcgcg
    17401 ttggtcgcgt ggttgatgcc caccgccgcg ttgcgcagcc gcgcgtcctt cggccagtcg
    17461 tggcggccgt ggggcatcat cgtgtaggag agtgcgtcgg cgaacggctg gccgtggaga
    17521 tcgtcgcccg cggtgaactg cccttcgttc gcctcgatgc cgcggtagaa gttgtagaag
    17581 gcgcgagtct cgggctcgga gttcgccgtg tagaaccggt ggctgaaccc gatgtcgggc
    17641 cagtcgcggt tcaggtccat cccgttgccg ttgtagcgct ggaagaagac gccaccctcc
    17701 tgcaccgagc cgcggcgcca gccgtccggg ttaggcagcg tgaagtagat gatcacgttg
    17761 cgcagcgtct cgccgaacgt cgcggactcg cccgcctcct tcggaagcag ccgcttgccg
    17821 ccggcgccgg tggtggccgc ggtgacgaga tcctcgatcg cgcggatgcc gccctcggcg
    17881 ccggcgcgct cgatcccgtg gatcgacagc gacagggcga agcgcttctt gcccttgtcc
    17941 ggcacggtct cgtcggtgac ccgcgccacg aacaggtccg ccttctggcg gctctgcgtg
    18001 gtggtgggca gaccggccga ctggtagcgc gagctcggct tgaactcgag acggccgagg
    18061 tcgttacccg ggaacatctc gttgccgggc ttggaccccg agccctcgcc gagcttcccg
    18121 tcgagcggcc agacctcgag atagcggcgc cactccggct tggagctcag gaactgcatc
    18181 ccgttgacga actcctggta gccgatgaac tgcgtggccg gggagttgcc ctgggcgaac
    18241 ggggagcaca cgcccgcgcc gggctggcat ccggcgagcg gatcggggaa gacgcgcccg
    18301 agctgcttgt aggagttctc gtcggtcgcg accggcccgg cgacagcggg cacggcgagc
    18361 acgagcagcg cgcacaccca cgccgccagc attcccccgc tccgccaccc ccgcttcacg
    18421 gcggcgcagt ctacgacccc ccggaccacc aagtcagcgg cgcctcgacg ggcggtagag
    18481 cctgaaggtc ttgcgcgccg tcccggtcag gccgtcgcgg aacttcaccg gcgccttcgc
    18541 gatcacgcgc gtgcggcgcc gctcgcggat gatcttccgt ccgctcttgg tgagccgcac
    18601 ccgcaggacc gtgttgcggg tcttggtgcg gatgttgaac ttcgcggcgc cgatcttcac
    18661 tgcccgcacg cgccgcttgc ccttcacgcg cacggcgcgc ttcacgtaca gcgtcagctt
    18721 cccgttgcac tggagcggcg tgcggcacga gacgcgcacg cccgcgtcgc ccttgcggct
    18781 catcttcacc gcgcgacccg agatcaggaa gcgcccgccg atcgtgccga cgccgccgcc
    18841 ggcgccccgg ttcggctggt cgagcaggtt ccgagcgacc gtgtagccgg tgtacgtcac
    18901 ggcgtagggc tcgatgcccc actcgtggtc gtactcctcg ctcggctggg gcagcagcgc
    18961 gccggcgatc cggatctgcc cgctcccgag cttcagctcg ccgatcgtga cctggtcgaa
    19021 gatcggcccg gcgtcgcgcg cccccgcgtc cgccgacgcc gcgacgctgc gcccgcccgc
    19081 cttctcccag gtctccttgt cgacggcgta ctggcgcgcg aacgaggcgt cgccgccgtc
    19141 ctcggtctgg atcgcgaagc cgagcggcgt gggctcgaac atctggcgcc ggaagccggt
    19201 gttgttgcgc gcgccgggcg gctgggtgtc gcgcagcagg acgtccttcg cggtgctcgg
    19261 gccgttcggg ttgaaggcga acgccgactg cccgacgtac acgcgctggc gctggacctt
    19321 gctgcccggg atcttcccgc cgggcacgag gtccggcagg gccctgagag cgccgtcggt
    19381 cagcacgagg ttgccgcctc cctcgacgaa cttgcgcagc gcgtcgagcc acttgtcctt
    19441 ctgctcgccg gtgaagtcgc tcggcggcgc ctgcccggcc ggcggcggcg cggtgaactc
    19501 gaccttcagc gtgaaggtgg ggtccggcgc ggcgaagttg atcatccgga ccacgtaggt
    19561 gccgggggag gggtcgggga tgatcacggt ctcgctggtc tgcggcgccc cctgcgcgga
    19621 cgagccctcc gaggccagcc cgcccgacgc ggtcttgcgg aacacgtaca tgtcccagtc
    19681 catcgccgtg ttcgtccagg tcatctggat cttcgcggac ttgttgctgt cgttcgggcc
    19741 gatcgtgaac tcgcggtcct cgtacgtgcc cgcgggcggg ttgcgcggcg cgccctggcc
    19801 gccacccggg gcggacgcgg ccgaggcgaa cgtctggttc ggcgtcggcc cgccggtcgg
    19861 gtggctgacg accccgccgt agtcgccggt gtagccgggc agcgcgtcgt cggcgagagc
    19921 gatcgtgtcg agcccggaca ggctctgcgt cccggcgatg accttcgcgg ggtcgatcgc
    19981 gcgcatgtcc tcgtcggtgc ccggcatgaa ctcgttgagg tccttgaaga agtcggtgtt
    20041 ggccacgtcg tacttgcgca gcacgggcgg gatgccgccg cccgtctcac cgctggccga
    20101 cgccggaccc tgcgtgaagt ggacctggac ccgagcggcc gcggtggggc cgtcgatgac
    20161 cgcccgccac tcgaccggct tgccgtcgcg cccgaacgcc tgcgggcggt tcaccgtcgc
    20221 gacgatcccg gactgggcgt agaggaacga ctggttgtag tcctcggcga ccgtcacgaa
    20281 cccgtcggcg tccttgaccc cgggatgctc gtcgcagttc ttgcactgga cctgcagcgt
    20341 cacctggccg cttccgacgc cctggacgtt cgagtccgtc acctcgatgc gcatgccgcc
    20401 gttgaagatg ttcttgccgg cgtcgtcgcc cgatccggcc ggctgctggc cctgcctcac
    20461 ggtgaacggg aactcgcctc cgcccggggt tgggtcgatg cccgagtcgt acgtaaactg
    20521 ggcgacgctg cccgcgggcg gccccggctg gttgctcttc tcgtcgcgcc tgaggcgctt
    20581 gttcggcacg tacgccttgc ggccgtctgc atcgaacacg gccgccgggg gcgggctcag
    20641 tatccgcgtc aggtgcgcgt agatcagcgc cttgttcccg tcgacgtgga gctgctcggt
    20701 gtgcgggtcg aagttgatgt tcttgtcgag gtgcgagaac gacatctcgt tgtcgatccc
    20761 gtccgcgttc aacccgacgg aggagtcgta ccagtccccg agggcgccgg tcgtcgtgta
    20821 gttgatcgtg tcgtacaccg tgccccaggt ctgggcgtag atctgcccgc acgccgtgcc
    20881 gagcgtcgtg tcctcgcacg tctcggtcgc gccgccgcgg ggcgagtcgt ccggcaccac
    20941 gatcggcgac cacttgatcg cgtcgtaggt cccgttgtgg atcgcgatcg cggcgtcctg
    21001 gatgcgcagg ttcttcgcgt agctgtggcg gccgtggggg aggagcgtgt acgagagcgc
    21061 gtccgcgaac ggctggccgt gcaggtcgtc accggcggcg aagcgcgggt ccgacttgcc
    21121 gttgacctgc gtgtagaagt cgatccacgc ctgggtctcc ggctctgaga tcgcgctgta
    21181 gccgcggtac gagaagccga tgtccggcca gtcgcggttc gggtcgacgc cgttgccgtt
    21241 gtagcgctgg aagaagacgc cgccggagga gaccgagccg cgccgccacc cgtccgggtt
    21301 cggcagcgtg aagtagatga tcgacttgcg caggacgtct gcgaacgtcg gcgcgttcgg
    21361 ctggaccgcc ttgggtgcga tcggctggtc ggccctctgc ttggtgaagg ccgtgacgag
    21421 atcctccatc gcgcggatgc cgccctccgc gcccgcgcgc tctatgccgt ggatcgagag
    21481 cgacagggcg tagcgcttct tgcccttgtc ggggacgttc tcgtcggtga cgcggatcgc
    21541 catcaggtcg gacttgcggc ggccgagctc ggtggtcggc aggccggcgg acacgtactt
    21601 cgcatcgggc ttgaactcga gcggcacgtt gttgttcggg tcgacgtcct tggtcgtcga
    21661 gcccgcgccg tcgccgagct tgccgtcgag ggcgacgatg tccatgtagc gctgccagtc
    21721 gcccttcgag ttcatgtagc gaatcgcgtc catgaactcg tcgatcccga tgaactgagt
    21781 cgcgggcacg ttgccctgcg cgttcggcga gcacggcgag gtgccggcgg cctggcagcc
    21841 cgcgagcggg tcggggaaca cacggccgag cgcggcgtag gcgccctcgt tggtcgcgac
    21901 cggcgtggcc gcggcgggcc cggcgaggaa gagcgcgcag agccagacgg cgccgaccgc
    21961 cgctgctcct acccccctga tccgcccgta cccccgcaaa gtcgccacgc attgaagcac
    22021 cgagcgttga cctctgcgcc agcttcggac gtttgggggg gttggacgaa cgccgctatc
    22081 ctgagcttcg atgcttcggt ccggatcgaa ctggccgacg agttgagggg ctcggcggca
    22141 tgaggaccct gcaccacccg cgcgtggacg agctggagct tccggaggtc ctgcacgcgc
    22201 tgagcgaccc ggtgcggctc gagatcgtcc gcctgctggc cgacgagacc gagcggccgt
    22261 gcagttccgt ggaggcgaag gtcgccaagt ccacgctctc gcaccacttc aaggtcctgc
    22321 gcgaggcagg aataacgtgg acaagggcga acgggacgca taggcttgtc tccctgcgga
    22381 ccgaggacct ggaggagcgt ttcccgggtc tcatcagctc ggtcctgaac gccgcgaagg
    22441 ccgcttagag cgcgaaagcg ccgagatggc aggcgcgcgc gggcggcaag ttcccgcaga
    22501 gtgcgccgtt tttacgaaat cggagcctgg gtaagcgtgt tccggaacac agatccgggg
    22561 taacaacctg gtaacgttct agatcagccg aactttcgaa atcttttcgc aactttcgcc
    22621 cccgaagcca gttggaaagc tgaatgcccg ctgtaactaa ggacaaccca caacacgccg
    22681 cgcgcaagcg ccacgccgac ggaggctccg cagctcgcaa gcgcgggggc gacgcggcgg
    22741 ccgagaagcc ggcccgcaag accaccaccc gcaagcgcgg agagaactcg caggacgagc
    22801 ccgcgcgtaa gaagactgag accgtgacca agaagaactc caagaccacc gctgcagaga
    22861 cgaagaagga gacgcccacg cggcgccgcc gtttcgcgcg tgaggacgac ggcatggccg
    22921 gcaagaccgc cgccgagaag gcgcacgcgc aggccgacga cgtcacgacc gactcgctcc
    22981 agctcttctt caacgaggcc cgcaagtacc cgctgctcac cgcggcggag gagatcgagc
    23041 tcgctcagcg catcgagaag ggcgatgtcg aggccaagga ccgcatgatc aactcgaacc
    23101 tgcggctggt cgtctcggtg gctcgcaagt accagggcca cggcctcacg ctgggcgacc
    23161 tcgtccagga gggcatgctc ggcctgattc gcgcgaccga gaagttcgac tggcgccgcg
    23221 gcttcaagtt ctcgacgtac gcgacgctgt ggatccgcca ggcgatccag cgcgggctgg
    23281 agaacagctc ccgcacgatc cgcctgcccg tccacatcgc gcagcgcgag cgcaagatca
    23341 accgcaccga gcgcgagctc gcgaccaagc tcggccgcga gccgaccgag gaggagctgg
    23401 ccgaggcggt cgagctcccg atcgaccagg tcatcgagat ccgcaaggcg gcgcggccgc
    23461 tcacgtcgct cgaccagccg gtcggcgacg acggcgagac cgcgttcggc gacctgctcg
    23521 cgagcaacgc gcccaacccc gaggacgagg tcgtccagga cgccggcacc agcgcggtgc
    23581 agcgcgccgt ggacgccctg cccgagcgcg agcgcgacgt ggtcgagctt cgcttcggcc
    23641 tcggcggtcg cgagcccacc ccgctgaggg agacgggccg ccggctcggc atctcggcgg
    23701 agcgggtgcg ccagatcgag gaagacgcgc tcgagcgtct tgccgagagc ggcgacctcg
    23761 ccgccctgcg cgacgcggcc tagccgcact gcgcacgacc gctgcgcggt aggcggggcc
    23821 tccgggcctc acgttttcac gccccgtagc gtttacgggg cgtgacgctg ccgccgtttc
    23881 aggcattcct cgacgctcac gccgaggcga tccaccgttt cctggtcgcg ctcgtcggcc
    23941 cgcaggaggc cgacgacgcg ctccaggaga ccctgatcgc cgcgctgcgg gcctacccgc
    24001 ggctgcggcc cgggtcgaat ctgcgcgcgt ggggactgtc gatcgctcgc aacaaagcca
    24061 tcgattccac gcgcgcgcgg gcgcgcaggc cggttccggt ggccgcgagc gtggaggtgg
    24121 ccgtgcccgc gggcgcggag ccggacggcg aggtgtgggc ggcggtccgc gtcctgccgg
    24181 ccaagcagcg cacggcgatc gccctgcgct acgcggccga cctgaggcat cgcgaggtcg
    24241 cgctcgcgat gggggtcagc gaggaggccg cgcggcggag cgtcagcgac ggcttgaaac
    24301 gactgaggga ggtcctcgga tgaaggaacg actgaccatt cgcagtccgc tgccggacgg
    24361 cgcgctggcc cggtttgctg agcgcgcggg ggaggagggc ctcgtcgacg tcgcctacgc
    24421 gacgctcgac acgccggtgg gcgagctgct cgtcgcgtcc acgcgccgcg gcgtggtgcg
    24481 catctcctac ctcgactggt tccccgccga ggcgaccatc gccgacctgg cccggcggat
    24541 ctcgccgcgc gtgctcgagg cgccggcacg gctcgacggg gtgcggcgcg agctcgacga
    24601 gtacttcgcc ggcgagcgcc gcagcttcga cgtgcgcgtc gatcgctcgc tgctcggtcc
    24661 gttcgccagg aaggtgctcg ggcgcacggc gcggatcccg tacggcgagg tctcgaccta
    24721 cgcggccatg gccgccgccg cgggcagccc gcgggcctcg cgcgccgcgg ggaacgcgct
    24781 cgcgtcgaac ccgatcccaa tcgtgatccc ctgccaccgc gtcctgcgca gcggcggcgg
    24841 gatgggcggc tacaccggcg ggctcgagcg caaggcgcag ctgctcgagc tcgaggggtg
    24901 gagcgccgtt tagattccgc ggctacgctc gtttcgtgcc ccggcgtctc acggtgatca
    24961 tcgccctggc cgcagtcgcg gccgtgctcg gcggcttcgt gctgctcacg ggcgggaagg
    25021 acacggtgcc gctctccacc gtcgcgaacg ctgcggaggc gacgaacagc gcgggcggct
    25081 tcaagctctc gatcgacggc agcttcgaga tcccgcaggt gaaccggacg ctgccgatga
    25141 agggctcggg ggcgctcgac ccgaaggcgc gccgcgggcg gctcgtcttc gactcggccg
    25201 gagcgctggc gcccacgggc ggggcgagcg gccggatcga gcaggtcttc gagggcgacg
    25261 tgatctacat gacgatgccg gcgatcgccc agcagctcgg cggcaagaag gcatggctgc
    25321 gggtggacat caaggaggcg gggcgcgcgc tcggcgtcga cccgtcgcag ttcagccagc
    25381 tcggtggcaa cgacccgcgg cagatgctcg accagatccg gtcggtcagc ggcgaggtgg
    25441 agaagctcgg cgccgagaag gtgcgcggtg tcgacacgac ccactaccgc gcctccgtgg
    25501 acctgcgcag gtacccggac cggctgcccg aggcccagcg cgagcaggcg cgcgtggcgg
    25561 tcgagcggct
//
LOCUS       DASVPL010000231         9877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_144583, whole genome shotgun sequence.
ACCESSION   DASVPL010000231 DASVPL010000000
VERSION     DASVPL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144583"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VF517_12410"
     CDS             complement(<1..340)
                     /locus_tag="VF517_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053787.1"
                     /translation="MADLERLMRIHNRSMVAFDLALGGGALAAPAATLRVLGHDPPSP
                     ETEELFRRLGPVWLTFAAAHMMAAVRGEPRDWWALAWLRGTEILTDVLWSSSPYFRRP
                     GARAGLWFAGA"
     gene            370..1647
                     /locus_tag="VF517_12415"
     CDS             370..1647
                     /locus_tag="VF517_12415"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX8053788.1"
                     /translation="MPGRIRITSGDDAGRIIEVTDELVVGRSQEGAGRIPDPEISRSH
                     ARVYLEPDGGLAIEDLGSTNGTFVSGTRITEPRSLSAGETLRVGQTTLEVEEAPEPAP
                     AADPELAPEDLGDIDPDIRGKDGPVADETVVGQPIPAEAEPSAEAEPEPAEPLPPQPP
                     SSGPPPPPGQAVPVGAAPVPSGLDAPPPFGAAAPPGAEPPPPGQAAPPGAAPPPPGQA
                     GPPGAAAPPAPAPSRLPRPLLAVLAGVLLLAAIGIGAVVLLTGDDEPEKKDKASETRV
                     EGPPALVSAARAAGCTAQNNPPEGRQHVRGVVKYRTNPPTSGNHDPDAAADGAYDTSP
                     PVAQLVHSLEHGRIVLWHKPSDRRARDMLLKVGDEDGSKMILVPNTTGMPYAVAATAW
                     GHLLGCPEANDKVPAAVRAFRDAYRGKGPELVP"
     gene            complement(1644..3014)
                     /locus_tag="VF517_12420"
     CDS             complement(1644..3014)
                     /locus_tag="VF517_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053789.1"
                     /translation="MTATRRTFSSGIAMLAVAVLAFAAPAGAAVTQMATGMPVGEVEL
                     AGQSVVFGSFGGGTARVMRADPGGSITKLAEHTVPGDDDDECCSTYFSTAFSASATQV
                     AFSSFYEAYVKGSLAQSNFKLSAGPPQGAVAQLFDCGLNHPYDVDGDRIAYLGDDCTE
                     KTSGLGARVVVRNLAAAGAPVVGSFAMTRQPNTLDLAGDHVALSGFFAAAPELIVYSL
                     SGAEQYKINHNFALYSLQADGKLAVAHPTAQGDCRLEWFSKAEPVAHRIDVCPRGRVR
                     MAGDRIAVDRLQGTTSSLDVVALNGSTRSVTFFQPSGALTGFDWDGSRLAYGVQSCVR
                     ADDAVFVEDLTSDPPVVEGGACTATIDTKKARADRKGLLRVRVSCPDGCTGELDLYRG
                     KTSVNRRTAAVSIRSGAKTIPIRLGTLRTVRPSGRVLQARLVVTQRSTATRTFKGNVR
                     VLPPKS"
     gene            3091..4758
                     /locus_tag="VF517_12425"
     CDS             3091..4758
                     /locus_tag="VF517_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX8053790.1"
                     /translation="MSARRRANLLLIVTDQQRAAMHWPDEPGWLRELTPTDHELARTG
                     LTFADAICSSCMCSPSRATLFTGLYPTQHRVTLTLTEAGARPDPRNAPAVFGTFAKSI
                     RSGSVPRGKTARAFARGALRIGANSGEAEPTLDPATPNLARLLAGAGYTVGFKGKWHL
                     TKPLHGEWSRDDADLLERDYGFAGWEPPDAGENLDPEHFGGGNAGSSGMGFDEDFTRQ
                     AEAFIATAAEPWALIVSLVNPHDVLGYPASYERGGYRAEEFRDLEVPLPATLEEDLSN
                     KPYPHSLMKLGQQSYIGPLRGRRAELDYLSFYAHLHRVVDAKIGRIVGALGDAGDARS
                     LRSRTVVVRTADHGEMGLAHGGLRQKMFNAYEETLRVPLVVSNPVLFPRAAETRAPAG
                     LVDLVPTLATLLGLGADGFAGADLTPVLARHADAPDEALEAAGVDLAAAARHPEPRDS
                     VQDASAFVYEDHKAGTAFENVVPQPNRIRALREPRWKYAAYVDPAGAAAPQYELYDLD
                     ADPIERHNLVDRTTGAVLDRRHEAERERLDALLHDRMQGVGAFMSGR"
     gene            4812..6731
                     /locus_tag="VF517_12430"
     CDS             4812..6731
                     /locus_tag="VF517_12430"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX8053791.1"
                     /translation="MRTLPLVSVVGALVIALLAPASAAGQLRDGLSTQLAIQDGPGGR
                     YQMGGDWLFRRDTADEGVLNRYFNHRGSSGWTKVQIPNAWNAQDESFESMNGDIVWYR
                     KDFRLPSEASGLDWIVRFQNVRYHAEVWLNGKQIGRHSGAYLPWELKLNGLRKGVNRL
                     VLRVDNRRFEDDLPPMQFDDTDQTPAGGWWNYGGMLGEVYLRRVNRIDMEEVEVRPTL
                     PSGATGPARVDWRVKLRNLHNRTQRVRVTGNYGGQRVNLGLKRIPAGGTSEARGRLTV
                     RSPRLWSPPDPQLYDVKITTDAGDAAAGSRRAAPLRRVGDYSLKSGIRSIRVENGRLL
                     VNDQPTNMRGVFVHEDDYVKGSALGNAERENMINRARDLGATLLRTHYPMHPQMHELA
                     DRLGVFIWSEVPVFQVPAELLRRSGLRATARDMLTTNVLTNQNHPSVLTWSVSNELRP
                     EPGSIERSYYKESSTLLRKLDPTRPISTVIAGYLDHEPQSSFSNFDMLGFNSYFGWYP
                     GRQASIADRENLSPFLDKMRQFYPSQAIMVTEHGAEANREGPAEERGTYAFQSEFYDY
                     HNRVYATKPWLSGVIGTLRAFRVRPGWEGGNPKPSNNWHEKGVLDFFDNPKPSYAVLQ
                     SWFRNTVQYGPGTPR"
     gene            6728..8632
                     /locus_tag="VF517_12435"
     CDS             6728..8632
                     /locus_tag="VF517_12435"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX8053792.1"
                     /translation="MTRRLLLLTLAVLALCAAPAQAAFFPGESIDGPADILRVTDIDT
                     AQDGGSAVVYLKRTEGVPHVWTVRMTNGIWGQPEQLDVGQAGESSDPHVAVSDGGRVV
                     AVWINGGRLFSAVRATGETPWTAPIEVHAGPVQHVSVGMSVHGVGYAAFSVGGASRDV
                     RAARLSGTTWTAFADPLDYEAERDAGGGRGPRIAAAADGTALAAWEEVAANGGRRVFV
                     RRVLRDRLSAYPAEVSVPQLEGRPGGAARNPEVGMDWDSSFGWVAVEQDFVDNGVNRS
                     RVFGRRIVGVGLEDPLLLDSLQWGGTDSATDPDLDVTGRLRALAASTLEPYDGVAGAV
                     LQIDVFGPIGRLDGPVGPVDPDPTVAHSSNGEGAFAWHEDANVHGRYWNRDEVLEGDT
                     PLANPEFGPVQAALGIDSSANRLGEVAIAFVQGGPLERRLVVSHWDRPLRAITPATAT
                     QNWQANRRPQLRWGRVTELWGSAQYRVEINGQPVVTTASTAFDLPFDLPDGSHPWRIV
                     TIDRRGQETPGLERRLNIDATRPVAELATTGTLRAGQSIRFVARDDPPPQPPPAAGAP
                     PPPAIRTSGLERVTASFGDRTRGTGTRELRHVFRKKGNYRVRLVVRDRAGNQAIVKLL
                     VKVANARRSR"
     gene            8629..>9877
                     /locus_tag="VF517_12440"
     CDS             8629..>9877
                     /locus_tag="VF517_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053793.1"
                     /translation="MSRLDRARGAPARRLTLARSAPARRLTLALLVVAAALGSAAPAQ
                     AARGMEIALQDDPVFLQRDYYDRDLALTQAREMGVTRLRVSLGWASALGTEQARQRTV
                     PPQPVYDFSRYDDLIDAAAGYGIRLQLNVIPPAPAWATGDRKVGVERPNPRLYGQFVR
                     AAAEHFKGRVDRFAIWNEPNHVGWLTPQSSAAAQYRQLYLEGYKAIKRVDPATEVLIG
                     ELVPYKNGSRSSSPLLFLRQLTCTRTASGVPGLRGYRMRRSGSCPPLRADGLAHHPYE
                     YRGPPESGYPGADNVTIGTLGRLTSTLSELGRLRALATPKRRPVDVFITEFGYFGVGR
                     NSHPDARRASYLRRAYQIAQRNPSVRQMTQYLLVRPPRRYTGSYFDMSIVLPNGEPSI
                     VFKELARWAKSAARRGYIAAPGQP"
BASE COUNT         1421 a         3765 c         3456 g         1235 t
ORIGIN      
        1 acgcgcccgc gaaccacagc cccgcccgcg cgcccggccg ccggaagtac ggcgacgacg
       61 accacaggac gtcggtgagg atctccgtcc cacgtagcca cgcgagcgcc caccagtcgc
      121 gcggctcgcc gcgcacggcc gccatcatgt gcgctgccgc gaaggtcagc cagacgggtc
      181 cgaggcggcg aaacagctcc tcggtctccg gcgacggcgg gtcgtgaccg agcacgcgca
      241 acgtcgccgc cggcgccgcg agcgccccgc ctccgagcgc caggtcgaag gcgaccatcg
      301 accggttgtg gatgcgcatg aggcgctcga ggtcggccac ttgcggcagc gtatagcttc
      361 cccgcgcgaa tgccgggacg aattcggatc acgagcggcg acgacgccgg ccggatcatc
      421 gaggtaaccg acgagctcgt cgtcgggcgc tcgcaggagg gcgcggggcg gatccccgac
      481 ccggagatct cgcgcagcca cgcgcgcgtc tacctcgagc ccgacggcgg cctcgcgata
      541 gaggacctcg gctccacgaa cggcacgttc gtgagcggca cgcggataac ggagccccgc
      601 tccctgagcg cgggggagac gctgcgcgtg gggcagacga cgctggaggt cgaggaggcg
      661 ccggagcccg cgccggcggc cgacccggag ctcgcccccg aagatctggg cgacattgac
      721 cccgatatac ggggcaaaga tggcccagta gccgacgaga cggtggtggg gcaaccgatc
      781 ccggccgaag cggagccctc ggccgaagcg gagccggagc cggcggagcc ccttcctccc
      841 cagccgccgt ccagcggccc gccgccaccg ccggggcaag ccgtgccggt cggagccgcg
      901 ccggtcccga gcgggctcga cgcgccgccg ccgttcggag ccgccgctcc ccccggagcc
      961 gagccgccac cgccgggcca agccgctccc cccggggccg ctcccccacc gccgggccaa
     1021 gccgggccgc ccggggccgc cgcgccgccc gcgcccgccc cgagccgcct cccccgcccg
     1081 ctcctcgccg tgctcgccgg ggtgctactc ctcgccgcca tcggcatcgg cgccgtcgtt
     1141 ctcctcaccg gcgacgacga gcccgagaag aaggacaagg cttcggagac gcgcgtcgag
     1201 ggaccgcccg ccctcgtcag cgccgcccgg gccgccggat gcacggcaca gaacaacccg
     1261 ccggagggac gccagcacgt gcgcggcgtc gtcaagtacc gcaccaaccc gccgacgtcc
     1321 ggcaatcacg atcccgacgc cgccgccgac ggggcctacg acacctcgcc gccggtcgcg
     1381 cagctcgtcc actcgctcga gcacggccgg atcgtcctgt ggcacaagcc gtccgaccgc
     1441 cgcgcgcgcg acatgctgct caaggtcggc gacgaggacg gcagcaagat gatcctcgtc
     1501 ccgaacacca caggcatgcc gtacgccgtg gccgccaccg cctggggcca cctgctcggc
     1561 tgcccggagg ccaacgacaa ggtgcccgcg gccgtccgcg ccttccgcga cgcctaccgc
     1621 ggcaagggcc cggagctcgt gccctagctc ttgggcggca gcacccggac gttgcccttg
     1681 aacgtccggg tcgccgtgct gcgctgggtc accaccagcc gtgcctggag cacccggccg
     1741 ctcggccgca ccgtgcgcag cgtcccgagc cggatcggga tcgtcttcgc gccgctccgg
     1801 atcgacaccg ccgccgtgcg ccggttcacg ctcgtcttcc cccggtagag gtcgagctcg
     1861 cccgtgcagc cgtcggggca cgagacccgc acccgcagga gccccttgcg gtcggcgcgc
     1921 gccttcttcg tgtcgatcgt cgccgtgcac gcaccgccct cgaccaccgg cgggtcgctc
     1981 gtcaggtcct cgacgaagac cgcgtcgtcc gcgcgcacgc agctctgcac gccgtacgcg
     2041 agccgcgagc cgtcccagtc gaagcccgtc agcgcgccgg aaggctggaa gaacgtgacg
     2101 gatcgggtcg agccgttcag cgcgaccacg tcgagcgacg acgtcgtccc ctggagccgg
     2161 tcgacggcga tccggtcgcc ggccatccgc acgcgcccgc gcgggcacac gtcgatccgg
     2221 tgcgcgaccg gctccgcctt cgagaaccac tcgagccggc aatcgccctg ggcggtgggg
     2281 tgggcgacgg cgagcttccc gtcggcctgg agcgagtaca gcgcgaagtt gtggttgatc
     2341 ttgtactgct cggcgcccga caggctgtag acgatcagct ccggcgccgc ggcgaagaag
     2401 cccgacagcg cgacgtgatc accggccagg tcgagcgtgt tcggctggcg cgtcatcgcg
     2461 aacgacccca cgaccggcgc gcccgccgcg gccaggttgc gcaccaccac gcgcgcgccc
     2521 aggccgctgg tcttctccgt gcagtcgtcg ccgaggtacg cgatccggtc gccgtccacg
     2581 tcgtacgggt ggttgagccc gcagtcgaag agctgcgcga ccgcgccctg cggcggcccg
     2641 gccgacagct tgaagttgct ctgcgccagc gagcccttga cgtaggcctc gtagaagctc
     2701 gagaacgcga cctgcgtggc cgatgccgag aaggcggtcg agaagtaggt gctgcagcac
     2761 tcgtcgtcgt cgtcgcccgg cacggtgtgc tcggcgagct tcgtgatcga gccgcccgga
     2821 tcggcgcgca tgacgcgagc ggtcccgccg ccgaacgagc cgaacacgac cgactgcccg
     2881 gccagctcca cctcgccgac cggcatgccc gtggccatct gcgtcacggc cgccccggcg
     2941 ggcgcggcga acgcgaggac cgcgaccgcg agcatcgcga tgccgctgga gaacgtcctg
     3001 cgggtggccg tcaccgggtt tcctttgctc gggggtaagc cgtttcgacc ctaagcgggg
     3061 accggtagcc ggtcaagtag cgtgcgcgcc atgtcggcga ggcggcgcgc caacctcctt
     3121 ctgatcgtca ccgaccagca gcgggcggcc atgcactggc ccgacgagcc cggttggctg
     3181 cgcgagctga cacccaccga tcacgagctt gcgcgcaccg gtctcacctt cgccgacgcg
     3241 atctgctcgt cgtgcatgtg ctcgccgagc cgcgcgacgc tcttcaccgg cctctacccc
     3301 acccagcaca gggtgaccct cacgctcacc gaggccggcg cccgccccga cccgcgcaac
     3361 gcgcccgcgg tgttcggcac gttcgccaag agcatccggt ccggctcggt gccgcgcggg
     3421 aagaccgccc gcgcgttcgc gcgcggggcg ctgcgcatcg gcgccaacag cggcgaggcg
     3481 gagccgacgc tcgatcccgc cacgcccaac ctcgcccggc ttctggccgg cgccggctac
     3541 acggtcgggt tcaagggcaa gtggcacctg acgaagccgc tccacggcga gtggtcgcgg
     3601 gacgatgcgg acctcctcga acgcgactac gggttcgcgg gctgggagcc gcccgacgcc
     3661 ggcgagaacc tcgaccccga gcacttcggc ggcggcaacg cgggcagctc ggggatgggg
     3721 ttcgacgagg acttcacccg ccaggccgag gccttcatcg cgaccgcggc cgagccgtgg
     3781 gcgctgatcg tctcgctcgt gaacccgcac gacgtgctcg gctacccggc ctcgtacgag
     3841 cggggcggct accgcgcgga ggagttccgc gacctggagg tgccgctgcc ggcgacgctc
     3901 gaggaggacc tctcgaacaa gccgtacccg cactcgctga tgaagctcgg gcagcagtcg
     3961 tacatcggcc cgctgcgtgg ccgccgggcc gagctcgact acctcagctt ctacgcgcac
     4021 ctgcaccgcg tggtggacgc gaagatcggc cgcatcgtgg gtgcgctggg cgacgccggt
     4081 gacgcgcgct cgctccgctc ccggaccgtg gtggtccgga ccgccgacca cggcgagatg
     4141 ggcctcgccc acggcggcct gcgccagaag atgttcaacg cgtacgagga gacgctgcgc
     4201 gtgccgctgg tcgtctcgaa cccggtcctg ttcccgcgcg cggccgagac gcgcgcgccc
     4261 gcggggctcg tggacctggt gccgacgctc gcgacgctgc tcggcctggg tgccgacggc
     4321 ttcgcgggcg ccgacctcac gcccgtcctc gcccgccacg ccgacgcgcc cgacgaggcg
     4381 ctcgaagcgg ccggcgtgga cctcgccgcc gccgcgcggc accccgagcc gcgcgactcg
     4441 gtccaggacg cgagcgcgtt cgtctacgag gaccacaagg cggggaccgc gttcgagaac
     4501 gtcgtcccgc agcccaaccg gatccgcgcg ctgcgggagc cgcgctggaa gtacgccgcc
     4561 tacgtcgacc cggcgggcgc cgccgcgccg cagtacgagc tctacgacct cgacgcggac
     4621 ccgatcgagc gccacaacct ggtcgaccgc acgaccgggg cggtcctcga ccgccgccac
     4681 gaggccgagc gcgagcgcct cgacgcgctc ctgcacgacc ggatgcaggg tgttggcgcc
     4741 tttatgtccg gccgctaact tcgcgcttcc ccctccgcaa caggcaggtc tcagctgtgt
     4801 tccaacgcgg gttgcgcacc ctcccgctcg tatcggtcgt aggcgcgctc gtgatcgcgc
     4861 tgctcgctcc ggcttcggcc gcgggccagc tccgtgacgg cctgagcacc cagctcgcca
     4921 tccaggacgg ccccggcggc cgctaccaga tgggcggcga ctggctgttt cggcgcgaca
     4981 ccgccgacga gggggtcctc aaccgctact tcaaccaccg cggctcgagc ggctggacca
     5041 aggtgcagat cccgaacgcc tggaacgccc aggacgagtc gttcgagagc atgaacggcg
     5101 acatcgtctg gtatcgcaag gacttccggc tgccctcgga ggcgagcggt ctcgactgga
     5161 tcgtccgctt ccagaacgtc cgctaccacg ccgaggtatg gctgaacggc aagcagatcg
     5221 gccgccactc cggcgcctac ctgccgtggg agctgaagct gaacggcctc cgtaagggcg
     5281 tcaaccggct cgtgctccgc gtggacaacc gccgcttcga ggacgacctg ccgccgatgc
     5341 agttcgacga caccgaccag accccagcgg gcggctggtg gaactacggc ggcatgctcg
     5401 gcgaggtcta cctgcggcgc gtgaaccgga tcgacatgga ggaggtcgag gtccggccca
     5461 cgctgccgag cggcgcgacc ggcccggcgc gggtggactg gcgggtcaag ctgcgcaacc
     5521 tccacaaccg cacccagcgc gtgcgcgtga cgggcaatta cggcgggcag cgggtcaacc
     5581 tcgggctcaa gcgcatcccg gccggcggca cctccgaggc acgcgggcgc ctgacggtgc
     5641 gcagcccgcg gctctggtcg ccgcccgatc cgcagctcta cgacgtgaag atcacgaccg
     5701 acgccggcga cgcggcggcg ggcagccgcc gggcagcacc gctgcggcgg gtgggcgact
     5761 actcgctgaa gagcggcatc cgctcgatca gggtggagaa cggacggctg ctcgtgaacg
     5821 accagccgac gaacatgcgc ggcgtcttcg tccacgagga cgactacgtg aagggctccg
     5881 cgctcggcaa cgcggagcgc gagaacatga tcaaccgggc gcgcgacctc ggcgcgacgc
     5941 tgctccgcac gcactacccg atgcacccgc agatgcacga gctggccgac cggctcggcg
     6001 tgttcatctg gtccgaggtg ccggtcttcc aggtgccggc cgagttgctc aggcgctcgg
     6061 gcctgcgcgc gaccgcgcgc gacatgctca ccacgaacgt cctgacgaac cagaaccacc
     6121 cgtcggtcct gacctggtcg gtctcgaacg agctgcggcc ggagccgggc agcatcgagc
     6181 gctcgtacta caaggagtcg tcgaccctgc tgcgcaagct cgacccgacg cgcccgatct
     6241 cgaccgtgat cgccggctat ctcgaccacg agccgcagtc gtcgttctcg aacttcgaca
     6301 tgctcgggtt caacagctac ttcggctggt accccggccg ccaggcctcg atcgccgacc
     6361 gcgagaacct ctcgccgttc ctcgacaaga tgcgccagtt ctatccgagc caggcgatca
     6421 tggtcaccga gcacggcgcc gaagcgaacc gcgaggggcc ggcggaggag cgcggtacct
     6481 acgcgttcca gagcgagttc tacgactacc acaaccgcgt ctacgcgacc aagccgtggc
     6541 tgtcgggcgt gatcgggacg ctgcgcgcgt tccgggtgcg gccgggctgg gagggcggca
     6601 acccgaagcc gtcgaacaac tggcacgaga agggcgtcct cgacttcttc gacaacccga
     6661 agccgtcgta cgcggtcctg cagagctggt tccgcaacac cgtccagtac gggccgggga
     6721 cgccgcgatg acccgccgcc tgctgctgct caccctggcc gtgctcgcgc tgtgcgcggc
     6781 accggcgcag gccgccttct tccccggcga gtcgatcgac gggcccgccg acatcctgcg
     6841 cgtgaccgac atcgacacgg cccaggacgg cggcagcgcg gtcgtgtacc tgaagcgcac
     6901 ggagggcgtc ccgcacgtgt ggacggtgcg gatgacgaac ggcatctggg gccagcccga
     6961 gcagctcgac gtcggccagg caggcgagtc cagcgacccg cacgtggcgg tctccgacgg
     7021 cggtcgcgtg gtggccgtgt ggatcaacgg cggccggctc ttctccgcgg tccgcgccac
     7081 gggcgagacg ccgtggaccg cgcccatcga ggtgcacgcg ggcccggtgc agcacgtctc
     7141 ggtcgggatg agcgtgcacg gggtcggcta tgcggcgttc tcggtcggcg gcgcctcgcg
     7201 cgacgtgcgc gccgcgcgcc tgtccggaac cacatggacg gccttcgccg acccgctcga
     7261 ctacgaggcc gagcgcgacg ccggcggcgg tcgcgggccg cgcatcgcgg cggcggcgga
     7321 cggcaccgcg ctcgcggcgt gggaggaggt cgcggcgaac ggcgggcggc gcgtgttcgt
     7381 caggcgcgtc ctgcgcgacc ggctcagcgc ctaccccgcg gaggtgagcg tgccgcagct
     7441 cgagggccgc ccgggcggcg cggcgcgcaa ccccgaggtc ggcatggact gggactcgag
     7501 cttcggctgg gtcgcggtcg agcaggactt cgtggacaac ggcgtcaacc gcagccgcgt
     7561 cttcggccgc cgcatcgtcg gcgtcggcct cgaggacccg ctgctgctcg actcgctcca
     7621 gtggggcggc acggacagcg cgaccgaccc ggacctcgac gtgaccggcc gcctgcgcgc
     7681 tctcgcggcc tccacgctcg agccgtacga cggcgtggcc ggggcggtgc tccagatcga
     7741 cgtgttcggt ccgatcggcc ggctcgacgg cccggtcggc ccggtcgatc ccgatcccac
     7801 cgtcgcgcac tcgtcgaacg gcgagggcgc gttcgcgtgg cacgaggacg ccaacgtgca
     7861 cggccgctac tggaaccgcg acgaggtgct cgagggcgat acgccgctcg ccaacccgga
     7921 gttcggcccg gtgcaggccg ccctgggcat cgactcctcc gccaaccgcc tcggcgaggt
     7981 ggcgatcgcg ttcgtccagg gtggcccgct cgagcggcgc ctcgtggtgt cgcactggga
     8041 ccgcccgctg cgcgccatca cccccgcgac cgccacccag aactggcagg ccaaccggcg
     8101 cccgcagctg cgctggggtc gcgtgaccga gctgtggggc tcggcccagt accgcgtgga
     8161 gatcaacggc cagcccgtgg tcacgaccgc gtcgaccgct ttcgacctgc cgttcgacct
     8221 gcccgacggg tcgcacccgt ggcggatcgt cacgatcgat cgccgcggcc aggagacccc
     8281 gggcctcgag cggcggctga acatcgacgc gacccgtccc gtcgccgagc tggccaccac
     8341 cggcacgctg cgcgccggcc agtcgatccg cttcgtcgcg cgcgacgacc cgccgccaca
     8401 gccgccgccc gccgccgggg cgccgccgcc gccggcgatc cgcacgtccg gcctcgagcg
     8461 cgtgacggcg tcgttcggtg accgcacgcg cggcaccggc acgcgcgagc tgcgtcacgt
     8521 cttcaggaag aagggcaact accgcgttcg cctggtcgtg cgcgaccgcg ccggcaacca
     8581 ggcgatcgtc aagctgctcg tgaaggtcgc gaacgcgagg cggagccggt gagccgcctc
     8641 gaccgcgcac gcggcgcccc ggcgaggcgg ctcacgctcg cacgcagcgc cccggcgagg
     8701 cggctcacgc ttgcgctcct cgtggtcgcc gcagcactcg gctccgcggc cccggcccag
     8761 gctgcgcgcg gcatggagat cgcgctccag gacgacccgg tgttcctcca gcgcgactac
     8821 tacgaccgcg acctcgccct gacccaggcg cgtgagatgg gcgtcacccg cctgcgcgtg
     8881 agcctcggct gggcgagcgc cctcggcacc gagcaggcgc gccagcgcac ggtcccgccg
     8941 cagccggtct acgacttctc gcgctacgac gacctgatcg acgccgccgc cggctacggc
     9001 atccggctcc agctcaacgt gatcccgccc gcgcccgcgt gggcaaccgg cgacaggaag
     9061 gtcggcgtcg agcgcccgaa cccgcgcctc tacggccagt tcgtgcgcgc cgcggcggaa
     9121 cacttcaagg ggcgcgtgga ccggttcgcg atctggaacg agccgaacca cgtgggctgg
     9181 ctcacgccgc agagcagcgc ggccgcgcag taccgccagc tctacctgga gggctacaag
     9241 gcgatcaagc gcgtcgatcc ggccacggag gtgctgatcg gcgagctcgt gccgtacaag
     9301 aacggctcgc gctcgagctc gccgctgctc ttcctccgcc agctcacctg cacgcgcacg
     9361 gcgtccgggg tgcccggcct gcgcggctac cggatgcgca ggagcgggag ctgcccgccg
     9421 ctgcgcgccg acggcctcgc gcaccacccg tacgagtacc gcggcccgcc cgagagcggc
     9481 tacccgggcg ccgacaacgt cacgatcggg acgctcggcc ggctcacctc cacgctgtcg
     9541 gagttggggc gcctgcgcgc gctcgccacg cccaagcggc ggccggtgga cgtcttcatc
     9601 accgagttcg gctacttcgg ggtggggcgc aacagccacc ccgacgcgcg ccgcgcgtcg
     9661 tacctgcggc gcgcttacca gatcgcacag cgcaacccgt cggtgcgcca gatgacccag
     9721 tacctgctcg tgcgcccgcc gcggcgctac accggcagct acttcgacat gagcatcgtg
     9781 ctgccgaacg gcgagccgtc gatcgtcttc aaggagctcg cgcgctgggc gaagagcgcc
     9841 gcgcggcgcg ggtacatcgc ggcaccgggg cagcccg
//
LOCUS       DASVPL010000232         5770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_382242, whole genome shotgun sequence.
ACCESSION   DASVPL010000232 DASVPL010000000
VERSION     DASVPL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382242"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VF517_12445"
     CDS             complement(<1..498)
                     /locus_tag="VF517_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053794.1"
                     /translation="MRLAYVLERYPELTQTFVEAELRELARTGHEPEVLALQPGDGAA
                     LAEAAFGPIYPARGAARVAALARTLARSPREAAAFVRGGEEARGRGPADGGGGGEARG
                     RGPAYAGGDRAAGAAPPSPAAVARAAADRWPPDNRRTRGLARIAAWHRAAARADHLHA
                     HFATEA"
     gene            complement(495..2165)
                     /locus_tag="VF517_12450"
     CDS             complement(495..2165)
                     /locus_tag="VF517_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053795.1"
                     /translation="MRRPDALPRPLVLALLFAGAALLSAITILQGVQPNDEGLMLQAA
                     QRIADGQVPYSDFWWYYPPGQPYLLAGLWDLFGPSLVVWRVVRVLTDAGVAVLAYELA
                     RRRAPGWLPLGVWLAAACAMAFPSQPHPFPIALALALGALIAVERRPLLAGVLLGACA
                     AWRLEFAAYAGLGIAIAHVVSSEPGRARITRLGRLVAAAVATGAALYLPVVLAAGVGR
                     SWEMLVEYPVLDFGDYQSLPFPLDYDGPLNTTGPGGFISDSAENLIHFYLPLALAIGL
                     AGGLLALALRLRRADPWPVATAVFAIGMGHYLLVRADIFHTAPLAVMVAILAAWALAA
                     SRAPRAHPSAETGQIVPLLNTPGEPKSKASPIGTQSTSGGAARRAIALAATAAATLGF
                     AWALAEGIDRRVRGLTDEDTVALDLPVADGVRARPPRALALERAARYVREHVPEGEPI
                     YVTTKRSDLVTSGNPLLYVLAERPNVTRYDIAAPGVVTSAPVQREIVADLRRERPLVV
                     RWIAPITAEPEPNRAGESSGVRILDDYLEREYRQVAKLGYYVMLEPAP"
     gene            2185..2715
                     /locus_tag="VF517_12455"
     CDS             2185..2715
                     /locus_tag="VF517_12455"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX8053796.1"
                     /translation="MGEREERTAVGGAAGRGEAARAAGAGEAAGPAGTGEAAAEPGAR
                     TAARRESHPEPSRLRELIEKLEDRREHHREHGTLYRVAFAVAGFTVLLGGIAMLLLPG
                     PALLVIPIGLAMLALEFAWAEEMLETAVEKAEAAGRAAEETSKKQRIVGFVAIGIAVA
                     AAVAAALVWDIPFLPV"
     gene            complement(2732..4324)
                     /locus_tag="VF517_12460"
     CDS             complement(2732..4324)
                     /locus_tag="VF517_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053797.1"
                     /translation="MVARIARRLGLDPFELGCLVVLVGTSFAVLLPLLSRGRPLSGAD
                     GLYPADQLQYFTWIREASQHGLIGNRMDLAPGDRPFLHPGFLLSGLLHRVTGISIPLS
                     YLVLWKPVAIGLTFAGALAYVRRLVPEGGQRRAALAIVLFAVMPASALVAWSDWGGNP
                     RQYTFDFISGEMWTGQFLWGYLFTAIAVFLMPLVLLAVERRRLGLAALGAFVVMYLQP
                     WQGATLALVVVAVELVQWRRSGERPPWAVALVPVAAGVPATYYFVLSHADESWKLASK
                     ANAAGGQPEWSWPWWAILLTLLPLAAPAALAYRLPAPSWQDVAVRIWPFAAFLVYLQP
                     GGTFPYHSFQGLAIPLGVLAVLGVASLSWRPSPALAVAAVAFLTLPGFAHKLETAANN
                     IHVGADPYFIFPDEHRGLDFVEGDPRPGGVLAPLYSGFYIPYTTGREVYVGQFSWAPD
                     ARTRRKRADDLFEGRLRGEPARAFVRSTRARFLFADCRKLSDLSGELRGMLEEVRRFG
                     CATVYVLRERPGMRAAAGRPDE"
     gene            4353..>5770
                     /locus_tag="VF517_12465"
     CDS             4353..>5770
                     /locus_tag="VF517_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8053798.1"
                     /translation="MSERLPFAASELRSRTIRGVAINAAFLVVVEAAALAQGLIVARI
                     LGPGEIGLYGIVSITVMTLIALKRVGIDEAFVQQDEEDQEREFQLAFSLELALAALFA
                     LLIALMAPLLVVVYDEPRLLPLTLALTYLPISFALQAPLWVFFRRMDFLRQRSLQAVV
                     PIVTFLVTVPLAIADVGVWSIVIGALAGNAASVALALRLSPYRLALRFERAAAARYFA
                     FSWPVLAVTAAGLVAGQGAILLFKLDGGLTAAGFLVLAVGFTRYADRAEHVLSPTVYP
                     VVCAVRDRTRALEELFTAAVRAAAIWSLGFGVMLVLFGPDLVDFLLGDEWEGATVLLQ
                     GLGAASGLYSLGYTWIAFARGLGRVRQPAVEALVAILAFGALAVPAYLAGGPTAYALA
                     ICGSSVTILVVRAYFVRRLLPGVDLGRLVLRCAWPVALAALPPIALRAALWGGERTGL
                     QAAAELALFVLVYAAIVVAAERP"
BASE COUNT          759 a         2153 c         2135 g          723 t
ORIGIN      
        1 ggcctcggtg gcgaagtgtg cgtggaggtg gtcggcgcgc gcggcggctc tgtgccaggc
       61 cgcgatacgg gcgaggcccc gcgtgcggcg gttgtcgggg ggccagcggt ccgccgcggc
      121 gcgcgcaacg gcggctgggg agggtggcgc ggcgcctgcc gcgcggtctc cgccggcgta
      181 cgccgggccc cggccccggg cttcgccccc tccgccaccg tccgccgggc cccggccgcg
      241 ggcttcctcc ccccctctca cgaacgccgc cgcttcgcgc gggctccgcg ccagggttcg
      301 cgccagcgcc gccacccggg ccgcaccgcg cgccgggtag atcgggccga aggcggcttc
      361 cgcgagcgcc gcaccgtcgc ccggctgcag cgcgagcacc tcgggctcgt gaccggtgcg
      421 ggcgagctcg cgcagctcgg cctccacgaa ggtctgcgtc agctcggggt agcgctccag
      481 cacgtacgcg agcctcacgg cgcgggctcc agcatcacgt agtagccgag tttcgcgacc
      541 tggcggtact cgcgctcgag gtagtcgtcg aggatgcgga cgccgctcga ctcgcccgcg
      601 cggttcggct cgggctccgc ggtgatcgga gcgatccagc gcaccacgag cggtcgctcg
      661 cgccggaggt cggcaacgat ctcgcgctgc acgggcgcgg acgtgaccac gccgggcgca
      721 gcgatgtcgt agcgggtcac gttcggccgc tcggcgagca cgtacagcag cgggttgccg
      781 gaggtgacga ggtccgagcg cttcgtggtc acgtagatcg gctccccctc cggcacgtgc
      841 tcgcggacgt agcgcgccgc gcgctccagt gcgagcgcgc gcggcggccg ggcccgcacg
      901 ccgtcggcca cggggaggtc gagcgccacg gtgtcctcgt cggtgagccc gcgcacgcgc
      961 cggtcgatcc cctcggcgag ggcccaggcg aagccgaggg tggcggcggc cgtggcggcg
     1021 agggcgatcg cgcgccgggc ggcgccgccc gaagtcgatt gcgtccctat tggggacgct
     1081 tttgacttcg gctcgccggg tgtgttcaaa agcgggacta tttgtcccgt ttctgcactc
     1141 gggtgggcgc gcggcgcgcg gctcgccgcc agcgcccagg cggccaggat cgccaccatc
     1201 accgccaggg gggcggtgtg gaagatgtcc gcgcggacca ggaggtagtg gcccatcccg
     1261 atcgcgaaga ccgccgtggc gaccggccac gggtcggccc gcctgaggcg cagcgccagc
     1321 gcgagcagcc cgccggccag cccgatcgcc agcgcaagcg gcaggtagaa gtggatcagg
     1381 ttctccgcgc tgtcggagat gaagccgccc gggccggtcg tgttcagggg accgtcgtag
     1441 tcgagcggga atggcagcga ctggtagtcg ccgaagtcga gcacggggta ctcgacgagc
     1501 atctcccacg agcggccgac gccggccgcc agcaccaccg gcaggtacag agcggccccg
     1561 gtggcgacgg ccgcggccac cagtcgcccg agccgcgtga tccgcgcacg gccgggctcc
     1621 gaagacacca catgggcgat cgctatgccg agccccgcgt acgcggcgaa ctcgagccgc
     1681 cacgcggcgc aggccccgag cagcacgccc gcgagcagcg gccgccgctc gactgcgatc
     1741 agggcgccga gcgccagcgc cagcgcgatc gggaacgggt gcggctggct cgggaacgcc
     1801 atcgcgcagg cggcggcgag ccacacgccg agcgggagcc agccgggcgc gcgccggcgg
     1861 gcgagctcgt acgcgagcac cgccacgccc gcgtccgtga gcacgcgcac cacgcgccag
     1921 accacgagcg aggggccgaa gagatcccac agcccggcga gcaggtacgg ctggccgggc
     1981 gggtagtacc accagaagtc gctgtacggg acctggccgt ccgcgatgcg ttgcgcggcc
     2041 tggagcatca ggccctcgtc gttcggctgg accccctgga ggatcgtgat cgcagagagc
     2101 agcgcggcgc ccgcgaacag cagggcgagc acgagcgggc ggggaagcgc gtcaggccgc
     2161 cgcaccgggg gtacggtatc cgtcatgggt gagcgggagg agaggaccgc ggtgggcgga
     2221 gccgcggggc ggggcgaagc cgcgcgagcc gcgggcgctg gcgaagccgc gggcccggcg
     2281 ggcacgggcg aggccgcggc cgagccgggc gccagaaccg ccgcgcggcg cgagagccac
     2341 ccggagccct ccaggctccg cgagctgatc gagaagctcg aggatcgccg cgagcaccac
     2401 cgcgagcacg gcaccctcta ccgcgtcgcg ttcgcggtcg ctggcttcac ggtcctcctc
     2461 ggcggcatcg ccatgctgct gctccccggc cccgcgctgc tggtcatccc gatcgggctc
     2521 gccatgctcg cgctcgagtt cgcctgggcg gaggagatgc tcgagaccgc ggtcgagaag
     2581 gccgaggccg cgggcagggc ggccgaggag acgagcaaga agcagcgcat cgtcggcttc
     2641 gtcgcgatcg ggatcgccgt ggccgccgcc gtggcggccg cgctcgtctg ggacatcccg
     2701 ttcctcccgg tctagcgagc gcgccagccg ctcactcgtc cggccgcccg gccgccgccc
     2761 gcatgcccgg ccgctcgcgc agcacgtaga ccgtcgcgca gccgaaccgc cggacctcct
     2821 cgagcatccc gcgcagctcg ccgctcaggt cactcagctt gcggcagtcg gcgaacagga
     2881 accgtgccct ggtcgagcgc acgaacgccc gcgccggctc cccgcgcagg cgcccctcga
     2941 acaggtcgtc cgcccgcttg cgccgcgtgc gcgcgtccgg cgcccacgag aactgcccca
     3001 cgtacacctc gcgcccggtc gtgtacggga tgtagaagcc cgagtagaga ggggcgagca
     3061 cgccgcccgg ccgcgggtcg ccctcgacga agtcgagccc gcggtgctcg tccgggaaga
     3121 tgaagtacgg gtcggcgccc acgtggatgt tgttcgcggc cgtctcgagc ttgtgcgcga
     3181 agcccggcag cgtgagaaac gccaccgcgg ccacggcgag ggccggcgac ggccgccacg
     3241 acagcgacgc gacgccgagc accgccagca cgccgagcgg gatcgcgaga ccctggaacg
     3301 agtggtacgg gaacgtgccg cccggctgca aatagaccag gaacgccgcg aacggccaga
     3361 tccgcaccgc cacgtcctgc cacgagggcg ccggcagccg gtacgccagc gccgccggcg
     3421 cggcgagcgg cagcagcgtc aggaggatcg cccaccacgg ccagctccac tccggctgcc
     3481 cgccggcggc gttcgccttc gacgccagct tccaggactc gtccgcgtgc gagagcacga
     3541 agtagtaggt cgccggaacc cccgccgcaa ccggcaccag cgcgaccgcc cacggcggcc
     3601 gctcgcccga gcgccgccac tggacgagct cgaccgccac cacgaccagc gccagcgtcg
     3661 ccccctgcca cggctggagg tacatcacga cgaacgcgcc gagcgccgcc agcccgagcc
     3721 tgcgccgctc caccgccagc agcaccagcg gcatcaggaa caccgcgatg gccgtgaaca
     3781 ggtagcccca gaggaactgg ccggtccaca tctcccccga gatgaagtcg aacgtgtact
     3841 ggcgcgggtt gccgccccag tcgctccagg ccaccagcgc cgaggcgggc atcaccgcga
     3901 acaggacgat cgcgagcgcg gcgcggcgct gcccgccctc cggcaccagc cgccgcacgt
     3961 acgcgagcgc gccggcgaac gtcagcccga tcgcgaccgg cttccacagc actaggtacg
     4021 acagcgggat cgagatcccg gtgacccggt gcagcagccc ggagagcagg aagcccgggt
     4081 gcaggaacgg gcgatcgccc ggcgcgaggt ccatccggtt gccgatcagc ccgtgctgcg
     4141 aggcctcgcg gatccacgtg aagtactgga gctggtcggc cgggtagaga ccgtccgcgc
     4201 ccgagagcgg gcggcccctg ctcagcagcg ggagcaggac cgcgaacgag gtcccgacca
     4261 ggacgacgag gcagccgagc tcgaacgggt cgagccccag gcggcgcgcg attcgggcga
     4321 ccacggcgga tgaccgtagt agcttggccc gcatgagcga gcggctcccg ttcgcagcct
     4381 cggagctgcg ctcgcgcacg atccggggcg tcgcgatcaa cgcggccttc ctcgtggtgg
     4441 tcgaggccgc cgcgctggcg cagggcctga tcgtcgcgcg gatcctcggc ccgggcgaga
     4501 tcgggctcta cgggatcgtg tcgatcacgg tcatgacgct gatcgcgctc aagcgcgtcg
     4561 gcatcgacga ggcgttcgtg cagcaggacg aggaggacca ggagcgcgag ttccagctcg
     4621 ccttctcgct cgagctcgcg ctggccgcgc tgttcgctct cctgatcgcg ctgatggccc
     4681 cgctcctggt ggtggtctac gacgagccgc ggctgctccc gctcaccctg gcgctgacct
     4741 acctgccgat ctcgttcgcg ctccaggcgc cgctctgggt cttcttccgc cgcatggact
     4801 tcctgcgcca gcgcagcctc caggcggtgg tcccgatcgt cacgttcctg gtgacggtgc
     4861 cgctggcgat cgccgacgtg ggcgtctggt cgatcgtcat cggcgccctc gccggcaacg
     4921 ccgcctcggt ggcgctggcg ctgcggctct cgccctaccg gctcgcgctg cgcttcgagc
     4981 gcgccgccgc cgcgcgctat ttcgcgttct cgtggccggt tctggcggtc accgcggccg
     5041 gcctagtcgc cggccagggc gcgatcctcc tcttcaagct cgacggcggc ctcaccgctg
     5101 cggggttcct cgtgctcgcg gtgggcttca cgcgctacgc cgaccgcgcc gagcacgtcc
     5161 tgtcgccgac ggtctacccg gtggtctgcg cggtccgcga ccgcacccgc gcgctcgagg
     5221 agctgttcac ggcggcggtg cgcgccgcgg ccatctggtc gctcggcttc ggcgtgatgc
     5281 tcgtgctgtt cggccccgac ctcgtggact tcctgctcgg cgacgagtgg gagggagcga
     5341 cggtcctgct ccagggtctc ggtgcggcca gcgggctgta ctcgctcggc tacacgtgga
     5401 tcgcgttcgc gcgcgggctc gggcgcgtgc gccagccggc ggtggaggcg ctcgtggcga
     5461 tcctcgcgtt cggcgccctg gcggtgcccg cttacctcgc cggcgggccg accgcctacg
     5521 cgctggcgat ctgcggctcc tcggtgacga ttctcgtcgt gcgcgcgtac ttcgtccgcc
     5581 ggctcctgcc aggtgtcgac ctcgggaggc tcgtgctgcg ctgcgcttgg ccggtcgccc
     5641 tcgcggcgct gcccccgatc gccctgcgcg ccgcactgtg gggcggcgag cgcacggggc
     5701 tccaggccgc ggccgagctc gcgctgttcg tgctcgtcta cgccgccatc gtcgtggccg
     5761 ccgagcggcc
//
LOCUS       DASVPL010000233         9289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_382256, whole genome shotgun sequence.
ACCESSION   DASVPL010000233 DASVPL010000000
VERSION     DASVPL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382256"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VF517_12470"
     CDS             <1..465
                     /locus_tag="VF517_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053799.1"
                     /translation="VFTQPLVADVQIFLAPPVKSGDAAGVVLYVKERSTGQQGTTTGR
                     IVKTSGSFGLEVRFEDLAGAQQAPEGFTVRIDRFQTDVGASRYEKVKVCCKTVKKNGK
                     KKKVKYLKKVRRDLIVNPKTCGGSWAYQVRLRYSATEESVRDGAAPCSASRR"
     gene            complement(478..2160)
                     /locus_tag="VF517_12475"
     CDS             complement(478..2160)
                     /locus_tag="VF517_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX8053800.1"
                     /translation="MRAEGRAPSPNGGAPRPTRGAPRPTRGAELYTDGRTAFLPSAVN
                     VEDGATIRGDRTVKVDVCVIGTGAGGAPVAKELAEGGMGVAMLEEGPHFTTSDFNARP
                     RDMLVELYRDAGQTATLGNTPILLPLGRSIGGTTLINSGTSFRTPEPVLEMWRERFGL
                     ESMSAAALDPIFRRVERIQNVVQVPPEYAGRNAHIVKRGAEALGWSGDFVYRNVRGCV
                     GSGICTFGCPTSAKQHVGLTYVPQAWEAGATTYTGCRATRLDVQNGRIRAVEARTSAG
                     GRLRVECDVAVVAAGAIATPLFLARQGLGGESGELGRNLSIHPASGVRALMDEEVDMA
                     KGVPQSYFIDEFADEGIMLEGAAGPPDYAAMSFPFSGERHRDLMLQYLHISQFGIMVS
                     DTSRGHVRERAGRVEIHYDLNAEDTATFKRGIGLLCELYFAAGARVVYAPVDRVAELR
                     DGDLRPLHDLDMRPSNLTLLAFHPMGTARADARPGSGVVDENLAVRGVEGVYVADASV
                     VPSSLGVNPQITIMTLATRLAFHLLGTAPPQDEPEPESIARPRITRPHALTA"
     gene            complement(2157..2585)
                     /locus_tag="VF517_12480"
     CDS             complement(2157..2585)
                     /locus_tag="VF517_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053801.1"
                     /translation="MPRTISGREASIFACFTDAVVAPEPFLPPVRETDAVAFFDRWMG
                     LLPRANRAGLRALLLLAEVAPLATHGGRLRRIDRGRRAEWVQRIERSGNGHVRMLMKL
                     LESAAQLSYYGNDAVLGQLGYDAEANLSRGRALRAAEGRP"
     gene            complement(2656..4506)
                     /locus_tag="VF517_12485"
     CDS             complement(2656..4506)
                     /locus_tag="VF517_12485"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX8053802.1"
                     /translation="MSWQLASFLVLGLALVAGFSWFERSRPPARVVSLVAALAALAVV
                     GRIVFAAIPNVKPTTDIVLFAGYTLGAAPGFAVGAVTALVSNFFISQGPWTPWQMAGW
                     GLVGISGALLARATGRRLGRIPLAVACGAAALLFGAVMNTHLWTLAASHDLGTWVAIS
                     GTSLPFDLAHVAGNVAFCLLLGPPFVRALERFRRRFEVHWRPAPAPAAAAVAAVIAGG
                     LLLPAPGEASPTSRAASYVRSAQNSDGGFGSSPGSSSNGLHTGWAALGLAAAGRNPAD
                     VGKGGKSVIDYIRANQASLNDLGELERTILVLRASSLSPRNFMGRDLVGQLKARQGGN
                     GSWAKNNAWTAFGILALRAAGESSGSSSVKRARTWLARQQGSDGGFGVAPGAASDTDN
                     TGSVLQALGASGLRSGKRVDRALRFLRASQNRDGGFGQLRDYDSNAQSTAWAVQGVLG
                     VGRSSGSLKRGSRTALGYIRSLQGSDGRIRYSRRSAQTPVWVTADALRALAGKPFPLR
                     PVARKRAAKRDPQSAARAKRRTAEHGGSGADGNGGGGGSGAAGGGGEPLGPPAPGAVK
                     AAARAPGSDGGEDGGGPSAALLLALTGAGLLLAAGVIWLVRRRLGDSLNS"
     gene            complement(4503..6029)
                     /locus_tag="VF517_12490"
     CDS             complement(4503..6029)
                     /locus_tag="VF517_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8053803.1"
                     /translation="MSPALRDVSLALEPGSFTVVAGASGSGKSTLLRAACGLVPHFHG
                     GSVEGSLVVGGLDVRDHGPGDLAAVCGTVLQEPETQVVMGGVRAEIALGLEHRGESAT
                     AVARAVEEVALMLGIAQLLDRRVETLSGGELQRVALAAALAGSPSLLVLDEPTSQLDP
                     VAGDELIWLLRRLNEEWGVAVMVAEHRLERCLPAADRVVAMEAGRVAFDGPPREFLAA
                     NPVTPAAQLFSRAGLTPLPVSVREARDALRLAGLLPAFLTYGSIRPKSQERRRFLRRR
                     PAAALDVRGVWHEIPDGPAILRGIDLRVEPGERVALMGRNGAGKSTLLRHVKGLVEPT
                     RGRIERGGEVALLLQNPGDYLVHERVEQELEAAGIEAAGLTGRERANPRDLSSGERQR
                     LALEIVLGETTPAVVCLDEPTRGMDQASKLSLIDRLSTLAAGGAAVVVATHDTELAAE
                     FADRIVLLGQGDVIADGPTAEVLAGGRHFATDVARILGGAALTPAAGALLIERELVAP
                     "
     gene            complement(6076..6951)
                     /locus_tag="VF517_12495"
     CDS             complement(6076..6951)
                     /locus_tag="VF517_12495"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX8053804.1"
                     /translation="MPSYRPGASALHAARASAGMALCGAYVLAAMLYDNPLVLTGALV
                     GVCAAAAGAGVIGELRRAAWLALPLAVLVVLINALVTRGGSTVIARGGEVLGRRLDIT
                     LEAVQYGGMSALRILVLVAAFALYSAAIDPDDVLRLLRRFSYRSALTVSLATRLVPVL
                     QRDAQRISEAACCRPVHPRRGAVAVAAITRALDRAVDVAASLELRGYAGARRPRRERR
                     PWSRHDLRVIAAAALVAGVAIGGKLLGAGGVDPYPTLDLAAGTPEALLAGALVLAGAA
                     PFAGSGARLGVARLG"
     gene            complement(6957..7970)
                     /locus_tag="VF517_12500"
     CDS             complement(6957..7970)
                     /locus_tag="VF517_12500"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEX8053805.1"
                     /translation="MSSPFRPARTACTPSARATSARSRRGSKSARQLLSAAAICAALI
                     LAACGVGPGKELDGAARLEVTRDFGQRFLGGTARIAVVHESDTVMRFLEKTHDVETSY
                     GGGFVDSIDGVRGDKAARRDWFYYVNGIEASKGAADFELSPGDRIQWDHRSWTAAMRV
                     PVIVGAYPEPMVNGQEGRRLPVRVQCERENSEACQGALRRLSDSGVIATQSSLQGPAG
                     EHSVRVLVGRWSALREVRGAAGIERGPRASGVFARFRGDSLELLDGDGRVARTAAPGT
                     GLVAATETGPSGIVWVVTGVDDAGVERAVEALDERKLHGAYAVAETERETTRLPIVEG
                     SGE"
     gene            complement(7879..8586)
                     /locus_tag="VF517_12505"
     CDS             complement(7879..8586)
                     /locus_tag="VF517_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053806.1"
                     /translation="MRTSAAAAVVAALMAVAPPAGAASYPKVEQLVVFRDGDSIQKTA
                     TAKAATVRVGGRSCTTGPSTPLAALVRIKPPKLGLRDYGSCSTRARDSAGLFVRSMNG
                     DTNKGFDGWVYKVGQKLATAGAADPSGPFGRGRLKTGQRVTWFYCRLQGQSCERTLVV
                     KSAPAATGQITVTVRAYDDRGKSIPAAGATVHADGSTATTGQDGSVVISLPPGTHSVY
                     AERAGDVRSFTERIEVG"
     gene            complement(8586..9269)
                     /locus_tag="VF517_12510"
     CDS             complement(8586..9269)
                     /locus_tag="VF517_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053807.1"
                     /translation="MKLRTLAVMAALAVLGALAPAGAQAVKVNVRVEGKSRTLLERNV
                     DTFVHPVTADASGPHKCDGTNAGASTTPGPTLTGAFDDAAKKESLDWAGSWSQSFEDF
                     LIDRVGPDAATSSQFWGTVLNFRDTDAGGCQVKVVEGDQVLIAFDSFGKTKLRLTGPR
                     GTRSGRAFALTVVDGNTGKAVSGARVAGRTTNAQGRVLLRITKRGLYRFKASASNAIR
                     SNQWKLRIK"
BASE COUNT         1259 a         3400 c         3464 g         1166 t
ORIGIN      
        1 gtcttcaccc agccgctcgt cgccgacgtg cagatcttcc tcgcgccacc ggtcaagagc
       61 ggcgacgccg ccggcgtggt gctctacgtc aaggagcgca gcaccggcca gcagggcacg
      121 accaccggcc ggatcgtgaa gacgagcggc tcgttcgggc tcgaggtgcg cttcgaggac
      181 ctcgccggcg cgcagcaggc gcccgagggc ttcaccgtca ggatcgaccg cttccagacc
      241 gatgtcggcg cgtcccgtta cgagaaggtc aaggtctgct gcaagacggt caagaagaac
      301 ggcaagaaga agaaggtcaa gtacctgaag aaggtccgcc gcgacctgat cgtcaacccg
      361 aagacgtgcg gcgggagctg ggcctaccag gtccggctgc gctactcggc gaccgaggag
      421 agtgtgcgcg acggcgccgc gccctgctcg gcctcgcgcc gctaggcccg ccgccgctca
      481 ggccgtgagc gcgtgcgggc gcgtgatgcg cggccgcgcg atcgactccg gctcgggctc
      541 gtcttgtggc ggcgcggttc ccagcaggtg gaacgcgagc cgcgtggcca gggtcatgat
      601 cgtgatctgc gggttcacgc ccagcgagct cggcacgacg ctcgcgtcgg ccacgtagac
      661 gccctcgacg ccgcgcaccg cgaggttctc gtcgaccacg ccgctcccgg ggcgcgcatc
      721 tgcgcgcgcg gtgcccatcg ggtggaacgc gagcagagtc aggttcgacg ggcgcatgtc
      781 gaggtcgtgc agcgggcgca ggtccccgtc gcgcagctcg gccacgcggt cgaccggcgc
      841 gtagacgacc cgtgcgccgg cggcgaagta gagctcgcag aggagcccga tgccgcgctt
      901 gaaggtggcg gtgtcctcgg cgttcaggtc gtagtggatc tcgacccgcc cggcgcgctc
      961 gcgcacgtgg ccgcgcgagg tgtcggagac catgatcccg aactgcgaga tgtgcaggta
     1021 ctggagcatc aggtcgcggt ggcgctcgcc gctgaacggg aacgacatcg cggcgtagtc
     1081 cggcgggccg gccgcaccct cgagcatgat cccctcgtcg gcgaactcgt cgatgaagta
     1141 cgactgcggg acgcccttcg ccatgtcgac ctcctcgtcc atcagcgcgc ggacgcccga
     1201 cgcggggtgg atcgagaggt tgcggcccag ctcgccggac tcgccgccga ggccctggcg
     1261 ggcgaggaac agcggggtgg cgatcgcccc ggcggccacc acggcgacgt cgcactccac
     1321 gcgcaggcgc ccgcccgcgg aggtgcgcgc ctcgacggcg cggatgcggc cgttctgtac
     1381 gtcgaggcgc gtggcgcggc agccggtgta ggtcgtggca ccggcctccc acgcctgcgg
     1441 gacgtaggtg aggccgacgt gctgcttcgc cgaggtcggg cagccgaacg tgcagatccc
     1501 ggagccgacg cagccgcgca cgttgcggta gacgaagtcg ccggaccatc cgagcgcctc
     1561 cgccccgcgc ttgactatgt gcgcgttgcg cccggcgtac tcgggcggga cctgcacgac
     1621 gttctggatg cgctcgacgc ggcggaagat cgggtcgagc gccgcggcgc tcatgctctc
     1681 gaggccgaag cgctcgcgcc acatctcgag caccggctcg ggggtgcgga acgaggtgcc
     1741 ggagttgatg agcgtggtgc cgccgatcga gcgcccgagc gggaggagga tcggcgtgtt
     1801 gccgagcgtg gcggtctgac cggcgtcgcg gtagagctcg accagcatgt cgcgcggtcg
     1861 ggcgttgaag tcgctcgtcg tgaagtgcgg gccctcctcg agcatcgcca cgcccatccc
     1921 gccctcggcg agctccttcg cgaccggcgc gccgcccgcg ccggtgccga tcacgcagac
     1981 gtccaccttg accgtccggt cgccgcggat ggtggcaccg tcctcgacgt tcacggcgct
     2041 ggggaggaac gcggtcctgc cgtcggtgta gagctcggcg ccgcgggttg ggcgcggggc
     2101 gccgcgggtt gggcgcgggg cgccgccgtt cgggctcggg gcgcgcccct ccgccctcac
     2161 gggcggccct cggcggcgcg gagcgcccgg ccccggctca ggttcgcctc ggcgtcgtac
     2221 ccgagctggc ccagcaccgc gtcgttgccg tagtacgaga gctgcgccgc gctctccagg
     2281 agcttcatca gcatccgcac gtggccgttg ccggagcgct cgatgcgctg tacccactcg
     2341 gcgcggcggc cgcggtcgat gcggcgcagg cgcccgccgt gcgtcgcgag cggcgcgacc
     2401 tcggccagca gcagcagcgc gcgcaggccg gcgcggttgg cccgtgggag caggcccatc
     2461 cagcggtcga agaacgcgac ggcgtcggtc tcgcggaccg gcggcaggaa cggctccggg
     2521 gccacgaccg cgtcggtgaa gcacgcgaag atcgacgcct cgcggccgga gatcgtgcgc
     2581 ggcatcgcgc cagcgtatcc cgtgccagcg cgccccctcc gtgcgctctg aacacttgat
     2641 gaccctatgg ggtcatcaac tgttcagaga atcgccgagc cggcggcgga ccagccatat
     2701 gacgcccgca gcgaggagca gccccgcacc ggtgagcgcg aggaggagcg cggcggaggg
     2761 cccgccgccg tcctcgccgc cgtctgagcc gggcgcgcgc gccgcggcct tgacggcgcc
     2821 gggggcgggc ggaccgagag gctcgccacc accaccggcg gcgccgctcc ctcccccgcc
     2881 gccgttgccg tcagcgccgg agcccccgtg ctcggcggtt cgccgcttcg cgcgagccgc
     2941 gctctgcggg tcgcgcttcg cggcccgctt gcgcgccacc ggtcgcagcg ggaacggctt
     3001 gccagccagt gcccggagcg catctgccgt gacccacacg ggcgtctgcg cgctgcggcg
     3061 cgagtatcgg atccgcccgt ccgagccctg gagcgagcgg atgtagccga gcgccgtgcg
     3121 cgagccgcgc ttcagcgagc cggagctgcg ccccacgccg agcactccct gcacggccca
     3181 ggcggtggac tgcgcgttcg agtcgtagtc gcggagctgg ccgaagccgc cgtcgcggtt
     3241 ctggctggcg cgcaggaagc gcagcgcgcg atccacccgc ttgccgctgc gcaggccgct
     3301 cgcgccgagc gcctggagca cgctcccggt gttgtcggtg tcgctggccg cgccgggcgc
     3361 cacgccgaag ccgccgtcgc tcccctgctg gcgcgcgagc cacgtgcgcg cgcgcttgac
     3421 cgaggacgag ccggagctct cgccggcggc gcgcagcgcg aggatcccga acgccgtcca
     3481 ggcgttgttc ttcgcccacg agccgttgcc gccctggcgc gccttcagct ggccgacgag
     3541 gtcgcgcccc atgaagttgc gcggcgagag ggacgaggct cgcagcacga ggatcgtgcg
     3601 ctcgagctcg ccgaggtcgt tcaggctcgc ctggttcgcg cggatgtagt cgatcacgga
     3661 cttgccgccc ttgccgacgt cggccgggtt gcgcccggcg gcggcgaggc cgagcgcggc
     3721 ccagccggtg tggaggccgt tcgacgagct gccgggcgag ctgccgaagc cgccgtcgga
     3781 gttctgggcc gagcgcacgt aggaggcggc cctgctcgtg ggcgaggcct cgcccggcgc
     3841 gggcaggagc agcccgcccg cgatcacggc cgcgacggct gccgccgccg gtgcgggagc
     3901 cgggcgccag tggacctcga agcggcgccg gaagcgctcg agcgcgcgca cgaacggcgg
     3961 accgagcagc aggcagaagg ccacgttgcc cgccacgtgg gcgaggtcga acgggagcga
     4021 ggtgccggag atcgcgaccc aggtgccgag gtcgtggctc gcggcgaggg tccacaggtg
     4081 cgtattcatc accgcgccga agaggagcgc cgccgccccg caggcgaccg cgagcgggat
     4141 gcggccgagc cggcggccgg tggcgcgcgc gagcagcgcg ccggagatcc caacgagtcc
     4201 ccagcccgcc atctgccagg gggtccacgg cccctgggag atgaagaagt tcgagacgag
     4261 ggccgtgacc gctcccaccg cgaagccggg cgccgctccg agcgtgtagc cggcgaagag
     4321 cacgatgtcg gtggtgggct tcacgttggg tatggcggcg aagacgatcc tgccgacgac
     4381 cgccagcgcc gcgagcgcgg ccacgagcga gaccacgcgc gcgggtgggc gcgagcgctc
     4441 gaaccagctg aagccggcca ccagcgcgag gcccagcacg aggaacgagg cgagctgcca
     4501 gctcacggcg ccaccagctc acgctcgatc agcagcgcgc cggccgcggg cgtgagcgcg
     4561 gcgccgccga ggatccgggc cacgtcggtg gcgaagtgcc ggccgccggc gagcacctcg
     4621 gcggtgggac cgtcggcgat cacgtcgccc tggccgagca gcacgatgcg gtcggcgaac
     4681 tcggcggcca gctcggtgtc gtgggtggcc accacgacgg cggcgccacc ggcggcgagg
     4741 gtcgaaaggc gatcaatcag ggagagcttc gacgcctggt ccatacctcg cgtgggctcg
     4801 tccaggcaga ccaccgccgg ggtggtttct ccgagcacga tctccagcgc caggcgctgg
     4861 cgctcgccgc tcgacaggtc gcgcgggttc gcgcgctcgc gcccggtgag gcccgcggcc
     4921 tcgatcccgg ccgcttcgag ctcctgctcc acgcgctcgt gcacgaggta gtcgcccggg
     4981 ttctggagga gaagcgcgac ctcgccgccg cgctcgatcc gcccccgcgt gggctcgacc
     5041 agccccttca cgtggcggag cagcgtgctc ttaccggcgc cgttgcggcc catgagggcc
     5101 acgcgctcgc cgggctccac gcgcaggtcg atcccgcgca ggatcgccgg cccgtccggg
     5161 atctcgtgcc agaccccgcg cacgtcgagc gcggcggcgg ggcggcggcg cagaaatcgt
     5221 cgacgttcct gacttttggg tcgtatggac ccataagtca ggaatgccgg aagaagtccc
     5281 gccaatcgca gggcgtcgcg cgcctccctc accgataccg gcagcggcgt gagccccgcg
     5341 cgcgagaaga gctgagcggc gggagtgacc ggattggccg cgaggaactc ccgcggcggt
     5401 ccgtcgaacg cgacgcggcc ggcctccatc gccacgaccc gatccgccgc gggcaggcag
     5461 cgctcgagcc ggtgctccgc caccatcacg gccacccccc actcctcgtt cagccgccgc
     5521 aggagccaga tgagctcgtc gccggcgacc gggtcgagct gcgacgtggg ctcgtcgagc
     5581 acgagtagcg acgggctccc cgccagcgcg gccgcgagcg ccacgcgctg gagctcgccg
     5641 cccgacagcg tctcgacgcg gcggtcgagc agctgcgcga tcccgagcat gagcgcgacc
     5701 tcctcgaccg cccgcgcgac ggcggtggcg ctctcgccgc ggtgctcgag cccaagcgcg
     5761 atctccgcgc gcacgccgcc catcaccacc tgcgtctcgg gctcctggag caccgtgccg
     5821 cagacggcgg cgaggtcgcc ggggccgtgg tcgcgcacgt cgaggccgcc gacgacaagc
     5881 gagccctcga cggagccgcc gtggaagtgc ggcacgagcc cgcacgcggc gcgcaggagc
     5941 gtggacttgc cggaccccga agcgccagcg acgacggtga aggaccccgg ctcgagcgcg
     6001 agcgagacgt cgcggagcgc gggcgacaca gcatccggat atccataggt gagccgttca
     6061 gcggtgaaca cggggtcagc caaggcgcgc cacccccagc cgcgcgcccg aacccgcgaa
     6121 cggcgcggcc ccggcaagca cgagcgcacc ggccagcagc gcctccggcg tccccgccgc
     6181 gagatcgagc gtgggatacg gatccacccc gccggccccg agcagcttcc cgccgatcgc
     6241 gacgcccgcc acgagagccg ccgcggcgat cacgcgcaga tcgtgccgcg accacggccg
     6301 gcgctcgcgc cgcggccgcc gcgcgcccgc gtagccgcgg agctccagcg aagccgcgac
     6361 gtcaacagcg cggtcgagcg cccgcgtgat cgccgcgacg gcgacggcgc cgcgccgcgg
     6421 atgcacgggc cggcagcagg cggcctcgga gatgcgctgt gcgtcgcgct gtagcaccgg
     6481 cacgagccgg gtggcgagcg acacggtgag cgcggatcgg taggagaatc ggcggagcag
     6541 gcgcagcacg tcatcggggt cgatggcggc ggagtagagc gcgaaggcgg ccacgagcac
     6601 gaggatgcgc agcgcgctca tcccgccgta ctggacggcc tcgagggtga tgtcgagccg
     6661 ccggccgagc acctcgccgc cgcgcgcgat gaccgtgctg cccccgcgcg tgacgagcgc
     6721 attgatcagc acgaccagca ccgccagcgg gagcgcgagc caggcggcgc ggcgcagctc
     6781 gccgatcacg ccggcgcccg cggcggcggc gcacacgccg acgagcgcgc cggtgagcac
     6841 cagcgggttg tcgtagagca tcgcggccag gacatacgcg ccgcagagcg ccatgcccgc
     6901 cgacgcgcgc gcggcgtgga gcgcgctcgc gccgggccgg tagctcggca ccagcctcac
     6961 tctcccgacc cctccacgat cggcagccgt gtggtctcgc gttcggtctc ggcgacggcg
     7021 taggcgccgt ggagcttgcg ctcgtcgagc gcctcgacgg cccgctccac ccccgcgtcg
     7081 tccactcccg tcacgaccca cacgattccg gacgggccgg tctcggtggc cgcgaccagg
     7141 ccggtgccgg gcgccgctgt gcgcgccacg cggccgtcgc cgtcgagcag ctcgagcgag
     7201 tcgccgcgga agcgcgcgaa cacgccgctg gcgcgggggc cgcgctcgat gcccgccgcg
     7261 ccgcggacct cgcgcagtgc cgaccagcgt cccacgagca cgcgcacgga gtgctcgccc
     7321 gcggggccct ggagcgagga ctgcgtggcg atcacgccgc tgtcggagag gcgccggagg
     7381 gctccctggc acgcctcgct gttctcacgc tcgcactgca cacgcacggg caggcgcctg
     7441 ccctcctggc cgttgaccat cggctccggg taggcgccca ctatcaccgg cacccgcatc
     7501 gccgcggtcc agctccgatg gtcccactgg atccggtctc ccggcgatag ctcgaagtcg
     7561 gcggcgccct tgctcgcctc gatcccgttc acgtagtaga accagtcgcg gcgcgcggcc
     7621 ttgtcgccgc gcacgccgtc gatcgagtcg acgaagccgc cgccgtagct cgtctcgacg
     7681 tcgtgggtct tctcgaggaa gcgcatcacg gtgtcgctct cgtggacgac cgcgatgcgc
     7741 gcggtcccgc cgaggaagcg ctggccgaag tcgcgcgtga cctcgagacg cgcggcgccg
     7801 tcgagctcct tccccggccc gacgccgcag gccgcgagga tcagtgcggc gcagatcgcc
     7861 gcggcgctga ggagttgcct agccgacttc gatcctctcc gtgaacgagc ggacgtcgcc
     7921 cgcgcgctcg gcgtacacgc tgtgcgtgcc gggcggaagg gagatgacaa ccgagccgtc
     7981 ctgcccggtg gtggcggtgg agccgtccgc gtggacggtc gcccccgcgg ctgggatcga
     8041 cttgccgcgg tcgtcgtacg cgcgcacggt cacggtgatt tggccggtcg ccgcaggcgc
     8101 cgacttcacc accagcgtgc gctcgcagct ctgtccctgg aggcggcagt agaaccacgt
     8161 gacgcgctgg ccggtcttca gccggccgcg gccgaagggg ccgctcgggt ccgcggcgcc
     8221 ggcggtggcc agcttctggc ccaccttgta gacccagccg tcgaagccct tgttcgtgtc
     8281 cccgttcatc gagcgcacga acaggccggc ggagtcgcgc gcgcgggtcg agcacgagcc
     8341 gtagtcgcgc aggccgagct tgggcggctt gatccggacg agggcggcga gcggcgtgct
     8401 cggcccggtg gtgcaggagc ggccgccgac gcgcacggtc gcggccttgg cggtcgccgt
     8461 cttctggatc gaatccccgt cgcggaacac gacgagctgc tcgaccttcg ggtagctcgc
     8521 cgcgcccgcc ggcggggcga cggccatgag ggccgccacc accgccgctg ccgcggacgt
     8581 gcgcatcact tgatccggag cttccactgg ttcgagcgga tcgcgttcga ggccgacgcc
     8641 ttgaaccggt acagcccgcg cttggtgatc ctcaggagca cgcggccctg ggcgttggtc
     8701 gtccggcccg cgacgcgcgc gcccgagacg gccttgccgg tgttgccgtc gaccaccgtc
     8761 agggcgaacg cccggcccga gcgcgtgccg cgcgggccgg tcagccggag cttcgtcttg
     8821 ccgaacgagt cgaacgcgat caggacctgg tcgccctcga cgaccttgac ctggcagccg
     8881 ccggcgtcgg tgtcccggaa gttcaggacg gtgccccaga actggctgga cgtggcggcg
     8941 tcggggccga cgcggtcgat caggaagtcc tcgaaggact ggctccagga gccggcccaa
     9001 tcgagcgatt ccttcttggc ggcgtcgtcg aaggcgccgg tgagggtcgg gccgggggtg
     9061 gtgcttgcgc ccgcgttggt gccgtcgcac ttgtgcgggc cgctggcgtc ggccgtgacc
     9121 gggtgaacga acgtgtcgac gttgcgctcg agcagagtcc tcgacttgcc ctccacccga
     9181 acgttgacct tgaccgcctg tgcgccggcg ggtgccagcg caccgagcac ggccagtgcg
     9241 gccatgacgg cgagagtacg gagcttcatg tgatttagcc tccccttcc
//
LOCUS       DASVPL010000234        16187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_264826, whole genome shotgun sequence.
ACCESSION   DASVPL010000234 DASVPL010000000
VERSION     DASVPL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16187
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264826"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VF517_12515"
     CDS             <1..1545
                     /locus_tag="VF517_12515"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8053808.1"
                     /translation="GVLATMGLSKLVSHIQPVASGIDSLILLVGLAVGVDYALFYLRR
                     SREYRAAGYSVDASVEAAAATSGRAVLVSGLTVLVSMAGMYLAGIPMFVSFATATIIV
                     VAVSVVAALTVLPAVLSKLGDRVEKGRIPFHGRLKRRTAELGVWSRITDRVLRRPKLA
                     VALTGGLLVALALPTLGMHTALSGTESMSRDIDVVRTWDRAQAAFPEESVPVVVVVRA
                     DDVTTGEARNALGKLESAAATRKDLYEGAATVETSDDRHVATMMFPAAGNGTDAESDR
                     ALDEMRDNLVPETVGRIDGAETVVTGETAAIRDFNGSLKSHLPLVFAFVLSMAFVLLL
                     LTFRSLVIPIKAIVLNLLSVGAAYGLLVAVFQKGWGESLLGFESNGAITAWLPPFLFV
                     VLFGLSMDYHVFILSRIREAFDSGMSTDEAVSHGIKSTAGVVTSAAIVMVGVFAIFGT
                     MSSIDMKQMGIGLSFAVLIDATLIRGVLLPASMKLLGDWNWWLPKRLAWIPELKHEGE
                     VGPAQA"
     gene            1670..1912
                     /locus_tag="VF517_12520"
     CDS             1670..1912
                     /locus_tag="VF517_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053809.1"
                     /translation="MASFLLEHAHGPDECDAAFAAWQGFSSPLRHQRVHSTCLSGGHG
                     LFWTVEAVDAEAALALLPRYVAARTRVIQVRDVVVP"
     gene            complement(1909..2652)
                     /locus_tag="VF517_12525"
     CDS             complement(1909..2652)
                     /locus_tag="VF517_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8053810.1"
                     /translation="MIEAERRDAVAVARLNRPEARNALSPEVMEELAALLEGWDFDPE
                     IRCIVIAGSDDYFAAGADIKAMAERSFQDVLASPSKAFWSRLAAIGTPLIAAVSGYAL
                     GGGCELALACDMIVAHEKAEFGQPEIMLGLIPGGGGTQRLTHTIGKQRTMELVLTGRR
                     ITAQEAQAFGLVNAIDKDWFGKAMEIAQVIARRPPIATRLAKEAVLTAEAADLTHERR
                     LFEIAMATEDRVEGMRAFVEKRKPDFRGR"
     gene            complement(2649..3806)
                     /locus_tag="VF517_12530"
     CDS             complement(2649..3806)
                     /locus_tag="VF517_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8053811.1"
                     /translation="MIDFELTDEQQLIRETARDFADREIIPVARDNDRNERFDLDLVK
                     KLGDMGYLGPIVAEEYGGRGLDYVTYGLIVEEIGRGDSSARTVVSVQTSLVCSSIQRW
                     GTEEQKQEWLPRLCSGEILGCFGLTEPDTGSDAANLKTRAEKIDGGWRISGQKQWISM
                     GNHAKVALLFAQTDPEQKHRGLAAFLVPTDSGGFSSGEIHGKLGLRASDTAELSLDGV
                     EVGDEALLGQVGDGFKVAMSALDSGRYSVAAGCVGICQGCVNASVAYSKERKQFDRPI
                     ASFQLVQAMIADMIVDTEASRALVWRAGWLKDAGKPNTTETSVAKLYATEAAVRTANT
                     AIQVHGGSGYVDDHPVERYLRDARVTTLYEGTSQIQKLIIGRAATGINAMT"
     gene            complement(3950..4948)
                     /locus_tag="VF517_12535"
     CDS             complement(3950..4948)
                     /locus_tag="VF517_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8053812.1"
                     /translation="MLAVRVSSPLVEAELAVSGVLALAGPSGAGKTTLLRMIAGLARP
                     DEGRIDCGRETWFGAGVDLPPERRRCGFVFQDHALFPHMSAVRNVTYGAHGDRARAVG
                     LLERLGVARAEARPRELSGGERQRVALARALASDPKALLLDEPLASLDPRTRARAARE
                     LSALLGDVPTIVVTHDFAEASLLASEVAVLDHGRIAQRGTPSELASSPASGFVADFTG
                     AVVLTGTASPGEAGLTRVDLDGGGSILSTDGAQGRVAVSIHPWDVTLGGTAGSARNRL
                     PAQVVTLTRIGNRTRVGLTTPQPLAAEVTTDSAARIRVGATVDATWKAAATRLLPL"
     gene            complement(4929..5687)
                     /gene="modB"
                     /locus_tag="VF517_12540"
     CDS             complement(4929..5687)
                     /gene="modB"
                     /locus_tag="VF517_12540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX8053813.1"
                     /translation="MRFVGAAALAIVLAFLALPVVAIFVDAGPGELLAALGDESALDA
                     LRLSLETSAIAVALVVTLGTPAAYLLATRAFRGRALVTTLVELPLVLPPAVAGVGLLA
                     ALGPNGIAGGPAPALVLETAGVVVALAFVSAPFYIRQAQAAFEAVDPALLEASRTLGY
                     GEAATLARVAIPVARPGLLAGVALALGRALGEFGATLMFAGSVRGVTQTAPLAIYERF
                     GTDFTGALALSAVLVALSLALLLTVKLSDARRTR"
     gene            complement(5684..6448)
                     /gene="modA"
                     /locus_tag="VF517_12545"
     CDS             complement(5684..6448)
                     /gene="modA"
                     /locus_tag="VF517_12545"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8053814.1"
                     /translation="MKPGGRGEARRRSRIGRLGSSLLGAAVLAAGAMGAAGCGGGDEP
                     RLTVSAASSLKTAFTEHAETFDGAKVRLSFGGSDQLAAQIRAGARPDVFAAANTELPR
                     QLHAEGLVEAPVRFASNRLVVAVRDDGGVRAVAELAEPGTKIAVGSPSVPVGSYTRGA
                     LRRLDPGVAANVASEEPDVSSIVARVRAGAVDAGFVYATDVRPLEELRAIELPFRTRV
                     EYAAAVVRGTKNTREARRFVRGLRGAKALRDAGFGP"
     gene            complement(6445..6834)
                     /locus_tag="VF517_12550"
     CDS             complement(6445..6834)
                     /locus_tag="VF517_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8053815.1"
                     /translation="MPRQAMRLGEAAQALGVSIDTLRRWDRAGKIRTIRDERNRRLVP
                     AAEVERLSARPRRHRTGEALSARNRFPGVVRSVEVDGVMGLVEIEAGGFRITAAITRD
                     AIEELGLGEGVRATATVKATSVMIEGG"
     gene            complement(6933..8147)
                     /locus_tag="VF517_12555"
     CDS             complement(6933..8147)
                     /locus_tag="VF517_12555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX8053816.1"
                     /translation="MADSRNAVVVDALRTPIGRYGGVLSTVRPDDLAAHVIEAAVERN
                     SLPAESIDDVFFGCTNQAGEDNRNVARMGLLLAGLPVEVPGATVNRLCASGLEAVNQA
                     ARAVVAGEGSLLLGGGVESMSRAPFAMLKPERSLPRGNQTLVDTTLGWRFVNARMAQR
                     YSTESMGETAENVAERYGVSREDQDAFALESHRRAAAAFEEGRFDDELVPVPVPQRKG
                     EPVVVHSDEGVRPDTTPEKLAALPAVFCEEGTITAGNSSQLNDGASCVVVASADRASE
                     LGREPLARIVSTGVAGVDPAYMGVGPVPASRLALERAGLSIEDIDLVELNEAFAAQVL
                     ASARELGIPHEKLNVNGGAIALGHPLGSSGARLLGTLIWELRRRGARYGLATMCIGVG
                     MGMAMVVENPAA"
     gene            8168..8518
                     /locus_tag="VF517_12560"
     CDS             8168..8518
                     /locus_tag="VF517_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053817.1"
                     /translation="MPVLPLAADDPYFGIAMVVMLVVLIGIFLAIGKWYPGSGADVLD
                     WQPTRSYEDEIRLELEDVDQMLEAQNERRRASGRPEISEDSVREEVETEEREQAERAA
                     AFRDRHGGQEPRNE"
     gene            complement(8537..9457)
                     /locus_tag="VF517_12565"
     CDS             complement(8537..9457)
                     /locus_tag="VF517_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8053818.1"
                     /translation="MRKPRHPGLHDAKGRELPHRLIQAGGVDTYAVDAGDGPPVVLVH
                     GYGDTADGWRRVVPGLLAAGHRVVAVDVPPFGRSGDPKTPSLLDFYKDFFPELFERLE
                     LERATVIGHSLGGAISLHLTLQRPDLVERLGLVAPAGLGKAPPWWWHLIAGYDVAWKT
                     ALSIPNPVTAPLIRHGMTRFLDWRLFHDPRQMRDTIDHLVSLHGTRKSFDLLLSAGRC
                     CIDSYTGTLLEDSRAIGVPLFMVWGEHDGLVPSRHASDFGRIHPNAHVHVFGDCGHYP
                     HIELPSRFNRVLREWLGAGGAGLRAAAEAA"
     gene            9527..9853
                     /locus_tag="VF517_12570"
     CDS             9527..9853
                     /locus_tag="VF517_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053819.1"
                     /translation="MARILIVGDDPALAAALGERGHAVSTVADPYPLGTLLPRLQGVS
                     ALAWLLGPDLLDSLAAKLVDTHVRGFACGPDGAEIAERFGQTYGMPTAVVRDGDWVAG
                     VESLIA"
     gene            complement(9843..10589)
                     /locus_tag="VF517_12575"
     CDS             complement(9843..10589)
                     /locus_tag="VF517_12575"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX8053820.1"
                     /translation="MSSPRPLIGLTTSEMRTPVRDELLPHADAGRQEMVLNFHYMRAV
                     TAAGGLPVVMSPQPLDAVGELVARLDGVLIPGGPDIDPSFYGHEPDDDLGDTWPEVDA
                     FEIAVVREAESRGLPVLGICRGMQLLNVAHGGTLYQHLPDHVGGEVLHRRLKLEDPVP
                     MHAVRIEAGSRLAEWLGRERAEVNSYHHQGPWEVGAGLRAVAWADDGVIEGLEGGNTL
                     GVQWHAEAMQEADEQRAMFAAFVAAATAKR"
     gene            10642..11490
                     /locus_tag="VF517_12580"
     CDS             10642..11490
                     /locus_tag="VF517_12580"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX8053821.1"
                     /translation="MAGYAKDVLVETDWVQEHLNDPNIRIVEVDENPALYAEAHIPGA
                     IGFDWKTDLQDQVKRDFLGPDEFGELMGARGISNGHTIVLYGDRNNWFAAYTYWYFRY
                     YGHDNVKLMNGPREKWIGEGRPTDTVTPEYQEETFKAQPGNEAIRAKRDEVLQALGSN
                     TRLVDVRSPAEFSGELIAMAGYEQEGAQRAGHIPGAASIPWAQAVKEDGTFKDADELR
                     ALYGGKGVTDGNDIIAYCRIGERSAHTWFVLHELLGQDNVKNYDGSWTEWGNMVNVPV
                     EKGPGS"
     gene            11490..12248
                     /locus_tag="VF517_12585"
     CDS             11490..12248
                     /locus_tag="VF517_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053822.1"
                     /translation="MRRLLALPLLVAAAVTGCGGGGDDDKPKTPKAAETIQQFQARLT
                     TAVAAIGQGQCETVTQFNSKAGFQLPCDERSKKLFAGFRVTGAKTYGSGGVVEFQDAE
                     TKGKFGVYTVAVGEDGKYQITGPISPIVEKSTLTEKADKAEEMDKSAQAMVDAIRSNN
                     CDKFFGAVITPPGMPKPQACKQELGEAYGPLRQQLAADKDAKPERLDGNAGFMFYALE
                     AGGQYRTLIVTRTEGAAKPYLGFVTFRGPAEKKT"
     gene            complement(12255..12764)
                     /locus_tag="VF517_12590"
     CDS             complement(12255..12764)
                     /locus_tag="VF517_12590"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX8053823.1"
                     /translation="MDAPGSTDAAVLIPLYETAGDLRAVFTRRRADLSRHAGEISFPG
                     GRQDSPDEDLRVTALRESEEEIGLPRDAVDLIGALPPVGTFVTSFRVFPFVGVIEAGH
                     RWTPEPREVDEVLELSLRALVEGHRMQRLLRKGVPIKTPTYTVGPHFVWGATARIVEH
                     LLDRLRPLL"
     gene            complement(12817..13212)
                     /locus_tag="VF517_12595"
     CDS             complement(12817..13212)
                     /locus_tag="VF517_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8053824.1"
                     /translation="MRAIARRQNGRFQHRIEMGRHWVTADEPEDDGGDDTGPSPQDLL
                     AGSLASCTAITIEMYAKRKGWDIGEVAVDVDYEPAQRGSPTKFEMKVLLPKELPEEQR
                     ERLMQIAAKCPVHRTLEGEVMFDESVELV"
     gene            complement(13209..13766)
                     /locus_tag="VF517_12600"
     CDS             complement(13209..13766)
                     /locus_tag="VF517_12600"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX8053825.1"
                     /translation="MVGIYTRKGDDGTTGLWYGGRVPKSDLRPEAYGAVDEAASMLGL
                     ARAAAGEEPEIHADIVRLQTELFVAGAELATAPEAAHRLEAGVSRVTEEMVDRLESDI
                     DRYMDRVELPPKFVLPGGTELSARLDVARAAVRRAERRVVDIDRAGALPDTTVLRFLN
                     RASDALYAMARFADEPEPELFGGRG"
     gene            13841..14950
                     /locus_tag="VF517_12605"
     CDS             13841..14950
                     /locus_tag="VF517_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX8053826.1"
                     /translation="MKRFLIALIAAVLALPAGANAASDMDFALQDDSVFVTNAYYGRE
                     RGLDKARDLGVTHIRVNVLWAEVVGSSKNRRSRPSTVNYDYTAYDQVVVAARARGMEV
                     QMALVGPAPAWATGNKKRGVYKPKYRYFEDFVERTVQHFGGLVNRYSIWNEPNHVGWL
                     APLKSGPGLYSQLYKVGYSAIKRNNPGAQVLFGELAPYASRRGSATPPLQFLRGVLRT
                     NRSYRGSSRYALEADGFAHHPYDFGHKPSYRYPGGDNVTLATLSRLTQALDRLAKYRM
                     LSTPSGDRLDVWLTEYGFMQSGRYAISDSRRASYLKAGFDMARRNGRVRQMLQFLLVQ
                     PSSRYRFFDTALLGRKGKETRTYRTIRSWAARYVR"
     gene            14971..>16187
                     /locus_tag="VF517_12610"
     CDS             14971..>16187
                     /locus_tag="VF517_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053827.1"
                     /translation="MRVRLALLAALAVLALPGSAMAASGMEVAIQDDSVFVAREYYKL
                     STALTQAQDLRASKIRVNVLWSSVVGAKSAKARRRPKKVRYDFTSYDQIVVAAQQRGM
                     QVQMALTGPAPAWAAGNRRSGPYKPNAKLYADFVKRTVAHFRATVGRFSIWNEPNHVG
                     WLAPVKSQAALYRSLYRAGYSAAKKANPGAQVLIGETAPYARDKRTAQPPLRFLRALL
                     CVNGNYAGTKCRLRADGYAHHPYEYSHGPDYQFPGGDNVTIGTLGRLTGALDRLAAGG
                     ALRTPAGGALDVYLTEFGYFRVGRFKLPDSTRGDYLRRAFEIAQANPRVRQMLHFLLV
                     EPPKKYAFFDTSIVSRKGRPSSAFSALAGWAQAAAADGRVGGYGGGGGGGGGSGGGGS
                     GGGGGEPPPPPTPP"
BASE COUNT         2334 a         5900 c         5706 g         2247 t
ORIGIN      
        1 ggcgtcctgg ccacgatggg gctgtcgaag ctcgtcagcc acatccagcc cgtcgccagc
       61 ggcatcgaca gcctgatcct cctggtcggc ctggccgtcg gcgtggacta cgcgctgttc
      121 tacctgcgcc gctcgcgcga gtatcgggcc gccggctaca gcgtggacgc ctcggtcgag
      181 gccgcggcgg cgacgtccgg ccgcgctgtg ctggtctcgg gcctgaccgt cctggtctcg
      241 atggccggca tgtacctcgc cgggatcccc atgttcgtgt ccttcgccac cgcgacgatc
      301 atcgtcgtcg ccgtgtcggt ggtggccgcg ctcacggtgc taccggcggt tctgtcgaag
      361 ctgggcgatc gcgttgagaa gggccggatc ccgttccacg gccggctcaa gcggcgcacg
      421 gcggagctcg gcgtctggtc gcgcatcacc gaccgcgtgc tgcgccggcc gaagctggcc
      481 gtcgctctca ccgggggtct cctggtggcg ctcgccctgc cgacgctcgg catgcacacc
      541 gcgctctcgg gcacggagtc gatgtcgcgc gacatcgacg tggtccgcac ctgggaccgc
      601 gcgcaggccg cgttcccgga ggagagcgtg ccggtcgtgg tcgtcgtcag ggccgacgac
      661 gtgacgacgg gcgaggcccg caacgccctc ggcaagctcg agagcgcggc ggccacccgg
      721 aaggacctct acgagggcgc ggccaccgtg gagaccagcg acgacaggca cgttgccacg
      781 atgatgttcc cggccgccgg caacggcacc gacgccgagt cggaccgggc gctcgacgag
      841 atgagggaca acctcgttcc ggagaccgtc ggccgcatcg acggtgccga gacggtcgtg
      901 accggtgaga ccgcggcgat ccgtgacttc aacggctcgc tcaagtcgca cctgccgctg
      961 gtgttcgcgt tcgtcctctc gatggcgttc gtgctcctgc tgctcacgtt ccgctcgctc
     1021 gtgatcccga tcaaggcgat cgtgctgaac ctgctgtccg tgggggccgc gtacggcctg
     1081 ctcgtggcgg tgtttcagaa gggctggggc gagtcactgc tcggcttcga gtcgaacgga
     1141 gcgatcacgg cgtggctgcc cccgttcctc ttcgtggtcc tgttcgggct ctcgatggac
     1201 taccacgtgt tcattctcag ccggatccgc gaggcgttcg actcgggcat gagcaccgac
     1261 gaggccgtgt cgcacgggat caagagcacg gccggcgtcg tcaccagcgc cgcgatcgtg
     1321 atggtcggcg tgttcgcgat cttcggcacg atgtcgtcga tcgacatgaa gcagatgggc
     1381 atcggcctct cgttcgccgt cctgatcgac gccacgctca tccgcggcgt cctgctcccg
     1441 gccagcatga agctgctcgg cgactggaac tggtggctgc cgaagcgcct ggcctggatc
     1501 cccgagctga agcacgaggg ggaggtgggg cccgcccagg catagccccg caacagcccc
     1561 gacgttcgag agcccgctcc ggcgggctct cggcgttccc ggctctcagg gtccgggttc
     1621 agggtcgcgc cccgatgtct cagggcgggc gcggagcgag tatccggtca tggcctcatt
     1681 cctcctcgag cacgcgcacg ggccggacga gtgcgacgcg gcgttcgcgg cgtggcaggg
     1741 gttcagcagc ccgctgcgcc accagcgcgt gcactccacc tgcctgtccg gcggccacgg
     1801 cctattctgg acggtcgagg ccgtcgacgc cgaggcggcg ctcgcactgc tcccgcgcta
     1861 cgtggccgcg cgcacccgcg tcatccaggt gcgcgacgtg gtggtcccct agcggcccct
     1921 gaagtccggc ttgcgcttct cgacgaaggc gcgcatgccc tcgacgcggt cctcggtggc
     1981 catcgcgatc tcgaagaggc ggcgctcgtg ggtcaggtcg gcggcctccg cggtgagcac
     2041 cgcctccttc gccaaccggg tcgcgatcgg cgggcggcgc gcgatcacct gagcgatctc
     2101 catcgccttg ccgaaccagt ccttgtcgat cgcgttcacg agcccgaagg cctgcgcctc
     2161 ctgcgcggtg atccgccggc cggtgagcac gagctccatc gtccgctgct tgccgatcgt
     2221 gtgcgtgagc cgctgggtgc ctccgccgcc ggggatcagc cccagcatga tctccggctg
     2281 gccgaactcg gccttctcgt gcgcgacgat catgtcgcag gccagcgcca gctcgcagcc
     2341 gccgcccagc gcgtagcccg acacggcggc gatcagcggc gtcccgatcg cggcgaggcg
     2401 cgaccagaat gccttgctcg gcgacgcgag cacgtcctgg aagctgcgct cggccatcgc
     2461 cttgatgtcg gcgcccgcgg cgaagtagtc gtcgctgccg gcgatcacga tgcagcggat
     2521 ctccgggtcg aagtcccagc cctcgagcag cgcggccagc tcctccatga cctcggggct
     2581 cagcgcgttg cgggcctcgg ggcggttgag gcgggcgacg gcgacggcgt cgcggcgctc
     2641 ggcctcgatc acgtcatcgc gttgatcccg gtcgccgcgc gcccgatgat cagcttctgg
     2701 atttgggatg tgccctcgta gagggtcgtc acacgagcat cgcgcaggta gcgctcgact
     2761 ggatgatcgt cgacgtagcc ggacccgccg tggacctgga tggcggtgtt ggcggtccgc
     2821 acggcggcct cggtcgcata cagcttcgcg acggacgtct cggtggtgtt cggcttgccc
     2881 gcgtccttca gccagcccgc ccgccagacc aaagcccgcg acgcctcggt gtccacgatc
     2941 atgtcggcga tcatcgcctg cacgagctgg aagctcgcga tcgggcggtc gaactgcttg
     3001 cgctccttcg agtacgccac cgacgcgttc acgcagccct ggcagatgcc tacgcacccg
     3061 gcggccacgc tgtagcgccc cgagtcgagc gcggacatcg cgaccttgaa gccgtcgccg
     3121 acctgtccga gcagcgcctc gtcgccgacc tcgacgccgt cgagcgacag ctccgccgtg
     3181 tcggaggcgc gcaggccgag cttgccgtgg atctccccgc tcgagaagcc gccggagtcg
     3241 gtgggcacga ggaaggcggc gaggccgcgg tgcttctgct cgggatcggt ctgggcgaag
     3301 aggagcgcga ccttggcgtg gttgcccatg gagatccact gcttctgacc gctgatgcgc
     3361 cagccgccgt cgatcttctc cgcgcgggtc ttgaggttgg cggcgtcgga gcccgtgtcg
     3421 ggctcggtca gcccgaagca gccgaggatc tccccgctgc acagacgcgg cagccactcc
     3481 tgcttctgct cctcggtgcc ccagcgctgg atcgagctgc acacgagcga cgtctggacg
     3541 gagacgacgg tgcgcgcgga ggagtcgccg cgaccgatct cctcgacgat cagcccgtag
     3601 gtcacgtagt cgagcccgcg gccgccgtac tcctcggcca cgatggggcc caggtagccc
     3661 atgtcgccga gcttcttgac gaggtcgagg tcgaagcgct cgttgcggtc gttgtcgcgg
     3721 gcgaccggga tgatctcgcg gtcggcgaag tcgcgggcgg tctcgcggat gagctgctgc
     3781 tcgtcggtca gctcgaaatc gatcacgtcg gcggacccta ccgcgcaact ccccaacctt
     3841 caggcgggcg cgtagttctg gtggttgtaa caccgccggg ggccgattcg gccatacccc
     3901 cctcggtctg cccccggcca gtaccccacg cgcccacccg cccgccccgc tacagcggga
     3961 gcaggcgggt ggccgcggct ttccaggtcg cgtcgaccgt cgcgcccacc ctgatccgcg
     4021 cagccgagtc ggtcgtgacc tcggcggcaa gcggctgcgg cgtcgtgagc ccgacacggg
     4081 ttcggttgcc gatccgcgtg agcgtcacga cctgcgcggg caggcggttc cgcgccgacc
     4141 ccgccgtgcc cccgagcgtc acgtcccacg gatggatgct caccgccacc ctgccctggg
     4201 cgccgtcggt gctcaggatc gagccgcctc cgtcgaggtc cacccgcgtc agtccggcct
     4261 cgccggggct cgccgtgccg gtgagcacca ccgcgccggt gaagtcggcc acgaagcccg
     4321 aggcggggga ggaggccagc tcgctgggcg tgccgcgctg cgcgatgcgg ccgtgatcga
     4381 gcacggcgac ctcgctcgcg agcagcgacg cctcggcgaa gtcgtgagtg accacgatcg
     4441 tgggcacgtc gccgagcagc gcggagagct cgcgcgcggc gcgggcacgg gtgcgcgggt
     4501 cgagcgaggc gagcggctcg tcgagcagca gcgccttcgg gtcggacgcc agagcgcggg
     4561 ccagcgccac gcgctggcgc tcgcccccgg agagctccct cgggcgtgct tccgcccgcg
     4621 cgacgccaag tcgctccaag agcccgacgg cgcgcgctct atccccgtgc gcgccgtagg
     4681 tcacgttgcg aacggcgctc atatgaggga acagtgcgtg gtcctggaac acgaacccgc
     4741 agcgccgccg ctccggcggc aggtccacac cggcgccgaa ccaggtctcg cggccgcagt
     4801 cgatccgtcc ctcgtccggc ctcgcgagcc cggcgatcat ccgcagcagc gtcgtcttac
     4861 cggcgcccga cgggcccgcg agcgccagga cgccagacac cgcgagctcc gcctcgacca
     4921 gcggcgagct aacgcgtacg gcgagcatcg ctcagcttca cggtgaggag cagcgccagc
     4981 gacagcgcga ccaggacggc cgagagcgcg agcgcgccgg tgaagtcggt gccgaagcgc
     5041 tcgtagatgg cgagcggcgc ggtctgcgtg acgccgcgga ccgagccagc gaacatgagc
     5101 gtggcgccga actcgccgag cgcgcggccc agcgcgagcg cgaccccggc cagcaggccg
     5161 ggccgcgcga ccgggattgc cacgcgcgcg agcgtggccg cctcgccgta cccgagcgtg
     5221 cgcgaggcct cgagcagcgc cgggtccacc gcctcgaacg ccgcctgcgc ctggcggatg
     5281 tagaacggcg ccgacacgaa cgccagcgcc acgaccacgc ccgcggtctc gagcacgagc
     5341 gccggggcgg ggccgccggc gatcccgttc gggccgagcg ccgcgagcag ccccacgccc
     5401 gcgacggccg gcggcagcac gagcggcagc tccacgagcg tcgtcacgag cgccctgccg
     5461 cggaacgcgc gcgtggcgag gaggtaggcg gcgggggttc cgagggtgac gaccagcgcg
     5521 acggcgatcg cgctggtctc gaggctcagc cgcagcgcgt cgagcgcgct ctcgtcaccg
     5581 agggccgcga gcagctcgcc ggggccggcg tcgacgaaga tcgccacgac cgggagcgcg
     5641 aggaaggcca gcacgatcgc gagcgcggcg gcgccgacga acctcatggc ccgaacccag
     5701 cgtcgcggag cgctttcgct ccgcgcagcc cgcgcacgaa ccgccgcgct tcgcgcgtgt
     5761 tcttggtgcc gcgcaccaca gccgccgcgt actcgacccg ggtgcgaaac gggagctcga
     5821 tcgcacgcag ctcctccaag ggccggacgt cggtggcgta gacgaacccg gcgtcgacgg
     5881 ccccggcccg cacccgcgcg acgatgctcg acacgtcggg ctcctcactc gcgacgttgg
     5941 cggcgacccc cgggtcgagc cgccgcagcg cgccgcgcgt gtacgagccg acggggacgg
     6001 acggggatcc gaccgcgatc ttcgtgccgg gctccgccag ctcggccacc gccctcaccc
     6061 cgccgtcgtc gcgcacggcc acgacgaggc gattgctcgc gaaccgcacc ggagcctcca
     6121 cgaggccctc ggcgtggagc tgtcgcggca gctcggtgtt cgccgccgcg aagacgtccg
     6181 ggcgtgcgcc ggcgcggatc tgggcggcga gctggtcgga gccgccgaag gagagccgga
     6241 ccttcgcccc gtcgaaggtc tcggcgtgct cggtgaacgc ggtcttcagc gagctcgcgg
     6301 cggagaccgt gaggcgcggc tcgtccccgc ccccgcaccc ggccgccccc atggctccgg
     6361 ccgccagaac ggcggcacct aggagggagg acccgagccg cccgatccgg ctccggcggc
     6421 gcgcctcgcc gcgaccgcct ggcttcaccc tccctcgatc atcaccgagg tcgccttaac
     6481 ggtcgcggtc gcgcgcaccc cctccccgag gccgagttcc tcgatggcgt cgcgggtgat
     6541 cgccgccgtg atccggaagc cgccggcctc gatctcgacc agtcccatca cgccgtcgac
     6601 ctcgaccgac cgcaccacgc ccgggaagcg gttgcgcgcg gacagcgcct ctccggtgcg
     6661 gtgccggcgc ggccgcgcgc tgagccgctc gacctccgct gctggcacga gccgccggtt
     6721 gcgctcgtcg cgaatcgtcc ggatcttgcc cgcacgatcc cagcgccgca gcgtgtcgat
     6781 cgacaccccg agggcctgcg ccgcctcgcc gagccgcatc gcttgcctag gcattgccta
     6841 tgcagactac agcgggccct cgacttctga acagttgata cgcctatagg ggcgtcaagt
     6901 gttcagaacg acgggcggag cgcggagggc gcctaggcgg cggggttctc cacgaccatc
     6961 gccatgccca tgccgacgcc gatgcacatg gtcgccaggc cgtagcgggc gccgcggcgg
     7021 cggagctccc agatcagcgt gccgagcagg cgggcgccgg aggagccgag tgggtggccc
     7081 agggcgatcg cgccgccgtt cacgttgagc ttctcgtgcg ggatgccgag ctcccgagcg
     7141 gaggcgagca cctgcgccgc gaacgcctcg ttcagctcga ccaggtcgat gtcctcgatc
     7201 gacaggccgg cgcgctcgag cgcgaggcgc gaagccggca ccggcccgac gcccatgtaa
     7261 gccgggtcga ccccggcaac cccggtggag acgatccgag caagcggctc acgcccgagc
     7321 tcggaagcgc ggtcggcgga agcgaccacg acgcacgacg ccccgtcgtt gagctgcgac
     7381 gagttccccg cggtgatcgt gccctcctcg caaaagaccg ccggcagcgc cgcgagcttc
     7441 tccggggtcg tgtccggccg gacgccctcg tccgagtgca cgaccacggg ctcgcccttg
     7501 cgctgcggca ccggtacggg caccagctcg tcgtcgaagc gaccctcctc gaaggccgcg
     7561 gccgcccgcc ggtgcgactc cagcgcgaac gcgtcctggt cctcgcgcga gaccccgtag
     7621 cgctcggcca cgttctcggc cgtctcgccc atcgactcgg tcgagtagcg ctgggccatc
     7681 cgcgcgttca cgaagcgcca gccgagcgtc gtgtccacga gcgtctggtt cccgcgcggc
     7741 agcgagcgct ccggcttgag catcgcgaac ggcgcgcggc tcatcgactc gacgccgccg
     7801 ccgagcagca gcgagccctc gcccgcgacc acggcgcgcg cggcctggtt gaccgcctcg
     7861 agtcccgacg cgcagagccg gttcaccgtc gcccccggca cctccaccgg caggcccgcc
     7921 agcagcagac ccatgcgcgc cacgttgcgg ttgtcctcgc cggcctggtt ggtgcagccg
     7981 aagaagacgt cgtcgatcga ctcggcgggc agcgaattgc gctccacggc cgcctcgatg
     8041 acgtgcgcgg cgagatcgtc gggacgcacc gtggagagca cgcccccgta gcgtccgatg
     8101 ggcgttcgga gtgcgtcgac cactaccgcg ttgcgcgaat cggccacctc cgcgatccta
     8161 ccgggagatg cccgtcctcc cgctcgccgc cgacgacccc tatttcggca tcgccatggt
     8221 ggtcatgctg gtcgtcctga tcgggatatt cctcgcgatc gggaagtggt acccgggctc
     8281 cggcgccgac gtgctcgact ggcagccgac ccgctcctac gaggacgaga tccggctgga
     8341 gctggaggac gtggaccaga tgctcgaggc gcagaacgag cgccggcgcg cgtcgggccg
     8401 cccggagatc agcgaggact cggtgcgcga ggaggtagag accgaggaac gcgagcaggc
     8461 tgagcgcgcc gcggcgttcc gcgaccgcca cggggggcag gaaccgcgga acgagtgacg
     8521 ggggcgccgt ccggcgctag gccgcttccg ccgccgcgcg cagccccgcg cccccggccc
     8581 cgagccactc gcgcagcacc cggttgaacc gcgacggcag ctcgatgtgc gggtagtgcc
     8641 cgcagtcgcc gaacacgtgc acgtgggcgt tcgggtggat ccggccgaag tcactcgcgt
     8701 gccgtgaggg caccagcccg tcgtgctcgc cccacaccat gaacagcggc accccgatcg
     8761 cgcgcgagtc ctcgagcagc gtgcccgtgt aggagtcgat gcagcagcgc cccgccgaga
     8821 gcagcaggtc gaacgacttg cgggtgccgt gcagcgagac gaggtggtcg atcgtgtcgc
     8881 gcatctggcg cgggtcgtgg aacaggcgcc agtcgaggaa gcgcgtcatg ccgtgccgga
     8941 tcagcggcgc cgtgaccggg ttggggatcg agagcgcggt cttccacgcg acgtcgtagc
     9001 ccgcgatcag gtgccaccac cacggcggcg ccttgccgag ccccgccggc gcgaccagcc
     9061 cgagccgctc gaccagatcc ggccgctgga gcgtgaggtg cagcgagatc gccccgccca
     9121 gcgagtgccc gatcaccgtg gcccgctcga gctcgagccg ctcgaacagc tccgggaaga
     9181 agtccttgta gaagtcgagc agcgaggggg tcttcgggtc gccggagcgc ccgaagggcg
     9241 ggacgtccac cgccaccacg cgatgccccg cggcgagcag ccccggcacc acgcgccgcc
     9301 agccgtcggc ggtgtcgccg tagccgtgga cgaggacgac cggtgggccg tcgccggcgt
     9361 cgaccgcgta cgtgtcgacg ccaccggcct ggatcagccg gtgaggtagc tcccgcccct
     9421 tcgcgtcatg gagccccgga tgtcgcggtt tccgcactct gcggtcgttc ccctcacgtc
     9481 ttttgtacca cggaatgggg acgtacgccg ctgccatgat gcgtacgtgg cgcgcatcct
     9541 catagtcggc gacgaccccg ctctggcggc cgcgctgggc gagcgcgggc acgccgtgag
     9601 caccgttgcc gacccctacc cgctcggcac cctgctgccg cggctccagg gcgtaagcgc
     9661 cctggcgtgg ctgctcggcc ccgacctgct cgactcgctg gccgccaagc tcgtggacac
     9721 gcacgtgcgt ggcttcgcct gcggcccgga cggcgccgag atcgccgagc gcttcggcca
     9781 gacctacggc atgccgacgg cggtcgtgcg cgacggcgac tgggtcgcgg gcgtcgagtc
     9841 gctcatcgct tagccgtagc cgcggccacg aacgccgcga acatcgcccg ctgctcgtcc
     9901 gcctcctgca tcgcctcggc gtgccactgg accccgagcg tgttgcctcc ctcgagcccc
     9961 tcgatcacgc cgtcgtcggc ccacgccacc gcgcgcagcc cggcgccgac ctcccacggc
    10021 ccctggtggt ggtacgagtt cacctcggcg cgctcgcgcc cgagccactc cgccagccgc
    10081 gacccggcct cgatccgcac cgcgtgcatc ggcaccgggt cctcgagctt cagccgccgg
    10141 tgcagcacct cgccgcccac gtggtccggc aggtgctggt agagcgtccc gccgtgggcg
    10201 acgttcagga gctgcatccc ccggcagatc ccgagcacgg ggagcccgcg ggactccgcc
    10261 tcccgcacga ccgcgatctc gaacgcgtcc acctcgggcc aggtgtcgcc caggtcgtcg
    10321 tcgggctcgt gcccgtagaa cgacgggtcg atgtccggcc cgccgggaat cagcacgccg
    10381 tcgaggcgcg cgaccagctc accgaccgcg tcgagcggct gcggcgacat caccaccggc
    10441 agcccgcccg cggccgtcac cgcccgcatg tagtggaagt tgaggaccat ctcctggcgc
    10501 cccgcgtcgg cgtgggggag cagctcgtcg cgcacgggcg tccgcatctc ggaggtggtc
    10561 aggccgatga gggggcgagg gctggacatt ggatgccatg atcccctatc cgaattcgag
    10621 acttcagacg gaggtagatc cgtggccggc tacgcgaagg acgtgctcgt cgagaccgac
    10681 tgggttcagg agcacctgaa cgatcccaac atacgcatcg tcgaggtcga cgagaacccg
    10741 gcgctctacg ccgaggcgca catccccggc gcgatcgggt tcgactggaa gaccgacctc
    10801 caggaccagg tcaagcgcga cttcctcggg ccggacgagt tcggcgagct gatgggcgct
    10861 cgcgggatct cgaacgggca cacgatcgtg ctctacggcg accgcaacaa ctggttcgcc
    10921 gcatacacgt actggtactt ccgctactac ggccacgaca acgtgaagct gatgaacgga
    10981 ccgcgcgaga agtggatcgg cgagggccgt ccgaccgaca cggtcacccc ggagtaccag
    11041 gaggagacgt tcaaggcgca gcccgggaac gaggccatcc gcgcgaagcg cgacgaggtg
    11101 ctccaggcgc tcggctcgaa cacgcggctc gtggacgtgc gctcgccggc cgagttctcc
    11161 ggcgagctga tcgcgatggc cggctacgag caggagggcg cgcagcgcgc cggccacatc
    11221 ccgggcgcgg cctcgatccc gtgggcgcag gcggtcaagg aggacggcac gttcaaggac
    11281 gccgacgagc tgcgcgcgct ctacggcggc aagggcgtga ccgacggcaa cgacatcatc
    11341 gcctactgcc ggatcggcga gcgctcggcg cacacgtggt tcgtgctgca cgagctgctc
    11401 ggccaggaca acgtgaagaa ctacgacggc tcgtggaccg agtggggcaa catggtcaac
    11461 gtgccggtcg agaagggccc gggctcgtag tgcggcggct gctggccctg ccgctgctgg
    11521 tcgcggcggc ggtcaccggc tgcggtggtg gcggggacga cgacaagccg aagaccccga
    11581 aggcggccga gacgatccag cagttccagg cgcgcctcac caccgccgtg gccgcgatcg
    11641 ggcagggcca gtgcgagacc gtcacgcagt tcaactcgaa ggcgggcttc cagctccctt
    11701 gcgacgagcg ctcgaagaag ctgttcgcgg gcttcagggt gaccggagct aagacctacg
    11761 gcagcggcgg cgtggtcgag ttccaggacg ccgagaccaa gggcaagttc ggcgtctaca
    11821 cggtcgcggt cggcgaggac ggcaagtacc agatcaccgg cccgatctcg ccgatcgtcg
    11881 agaagagcac cctgacagag aaggccgaca aggccgagga gatggacaag tcggcgcagg
    11941 cgatggtcga cgcgatccgc tcgaacaact gcgacaagtt cttcggcgcg gtgatcacgc
    12001 cgcccgggat gccgaagccg caggcctgca agcaggagct cggcgaggcc tacgggccgc
    12061 tgcgccagca gctcgcggcc gacaaggacg cgaagcccga gcgcctcgac ggcaacgccg
    12121 gcttcatgtt ttacgcgctc gaggccggcg gccagtaccg cacgctgatc gtgacgcgca
    12181 cggagggggc ggcgaagccg tacctcggct tcgtgacctt ccgcggcccg gcggagaaga
    12241 agacctagaa gccctcagag cagcgggcgc agccggtcga gcaggtgctc gacgatgcgc
    12301 gcggtcgcgc cccacacgaa gtgcggcccg acggtgtagg tgggggtctt gatcgggacc
    12361 cccttgcgca gcaggcgctg catccggtgt ccctccacga gcgcgcgcag cgacagctcg
    12421 agcacctcgt cgacctcgcg cggctccggc gtccagcggt ggccggcctc gatcacgccg
    12481 acgaacggga acacgcggaa gctggtgacg aacgtgccca ccggcggcag cgcgccgatc
    12541 aggtcgacgg cgtcgcgcgg caggccgatc tcctcctcgc tctcgcgcag cgcggtcacg
    12601 cggaggtcct cgtcggggga gtcctggcgc ccgccgggga acgagatctc gccggcgtgg
    12661 cggctcaggt cggcgcggcg cctggtgaat accgcgcgca ggtcgccggc ggtctcgtag
    12721 agggggatca ggacggcggc gtcggtcgag ccgggcgcgt ccatggcggc ggcctcctcg
    12781 ggggtgagga gcagatcgcc cgcccgcgcg agcgggctag acgagctcga ccgactcgtc
    12841 gaacatcacc tcgccctcga gcgtccggtg gaccgggcac ttcgcggcga tctgcatcag
    12901 ccgctcgcgc tgctcctccg gcagctcctt gggcaggagc accttcatct cgaacttggt
    12961 gggcgagccg cgctgggccg gctcgtagtc cacgtccacg gccacctcgc cgatgtccca
    13021 gcccttgcgc ttggcgtaca tctcgatcgt gatcgccgtg cacgaggcga ggctcccggc
    13081 gagcaggtcc tggggactcg gcccggtgtc gtcgccgccg tcgtcctccg gctcgtccgc
    13141 ggtgacccag tgccggccca tctcgatcct gtgctggaac ctgccgttct gacggcgtgc
    13201 gatcgctctc atcctcttcc tccgaacagc tcgggctccg gctcgtcggc gaagcgggcc
    13261 atggcgtaca gggcgtccga ggcgcggttc aggaaccgca gcacggtcgt gtcgggcagc
    13321 gcgccggcgc ggtcgatgtc gaccacccgg cgctcggcgc ggcggacggc cgcgcgcgca
    13381 acgtcgagac gggcggagag ctcggtgccg cccggcagca cgaacttcgg cggcagctcc
    13441 acgcggtcca tgtagcggtc gatgtcggac tcgagccggt ccaccatctc ctcggtcacc
    13501 cgcgagaccc cggcctcgag ccggtgcgcg gcctcgggcg cggtggccag ctcggcgccg
    13561 gccacgaaca gctctgtctg gaggcggacg atgtcggcgt ggatctcggg ctcctcgccc
    13621 gccgccgcgc gtgccagccc cagcatcgag gcggcctcgt cgacggctcc gtaggcctcc
    13681 gggcggaggt ccgacttggg cacgcggccg ccgtaccaga ggccggtcgt gccgtcgtcg
    13741 cccttgcgcg tgtagatgcc caccatcggg cgctgatcct agggagtctg catgctgaca
    13801 gctccgaacg cagggaaaac gagttgctac atctgagctc atgaagagat tcctgatcgc
    13861 gctcatagcc gccgtcctcg cgctgcccgc cggcgccaac gccgcatccg acatggattt
    13921 cgcgctccag gacgactcgg tcttcgtcac gaacgcgtac tacggccgcg agcgcgggct
    13981 cgacaaggcc cgcgacctcg gcgtgaccca catccgcgtg aacgtgctct gggccgaggt
    14041 ggtcggcagc tcgaagaacc gccgctcgcg cccgagcacg gtcaactacg actacacggc
    14101 ctatgaccag gtcgtggtcg ccgcccgcgc tcgcggcatg gaggtccaga tggcgctcgt
    14161 cggcccggcc cccgcgtggg ccaccggcaa caagaagcgc ggcgtctaca agcccaagta
    14221 ccgctacttc gaggacttcg tggagcggac cgtccagcac ttcggcgggc tcgtgaatcg
    14281 ctactcgatc tggaacgagc ccaaccacgt cggctggctc gcgccgctga agagcggtcc
    14341 ggggctctac agccagctct acaaggtcgg ctacagcgcg atcaagcgca acaaccccgg
    14401 cgcccaggtc ctgttcggcg agctggcccc gtacgccagc cgccgcggct cggccacgcc
    14461 gccgctccag ttcctgcgcg gcgtgctgcg cacgaaccgc agctacaggg gcagcagccg
    14521 ctacgcgctc gaggccgacg ggttcgccca ccatccgtac gacttcgggc acaagccgag
    14581 ctaccgctat ccggggggcg acaacgtcac gctcgccacg ctgagccggc tcacccaggc
    14641 cctcgaccgg ctggcgaagt acaggatgct gagcacgccc agcggcgacc ggctcgacgt
    14701 gtggctgacc gagtacggct tcatgcagtc cggtcgctac gcgatctcgg actcgcgccg
    14761 ggcctcgtac ctgaaggcgg gcttcgacat ggcccgcagg aacgggcggg tgaggcagat
    14821 gctccagttc ctgctcgtgc agccgagctc gaggtaccgc ttcttcgaca ccgccctcct
    14881 cggccgcaag ggcaaggaga cgcgcaccta caggacgatt cggtcgtggg cggcgcgcta
    14941 cgtgcgctga cgcgctctaa ttcagggaca gtgcgcgtca gactggccct gctcgccgcc
    15001 ctagcggtgc tcgccctgcc cggctcggcc atggccgcgt ccgggatgga ggtcgcgatc
    15061 caggacgact cggtcttcgt cgcccgcgag tactacaagc tctccacggc gctgacccag
    15121 gcccaggacc tgcgggcgtc gaagatccgg gtgaacgtgc tgtggtcgtc ggtggtgggc
    15181 gcgaagtccg cgaaggcgcg ccgcaggccg aagaaggtcc gctacgactt cacctcgtac
    15241 gaccagatcg tggtggccgc gcagcagcgc gggatgcagg tgcagatggc gctcaccggc
    15301 ccggcgcccg cgtgggcggc gggcaaccgc cggagcggcc cgtacaagcc gaacgccaag
    15361 ctctacgcgg acttcgtcaa gcgcacggtc gcgcacttcc gcgcgaccgt cggccgcttc
    15421 tcgatctgga acgagccgaa ccacgtcggc tggctcgcgc cggtcaagag ccaggccgcg
    15481 ctctaccgct cgctctaccg ggcgggctac tcggccgcga agaaggccaa ccccggcgcg
    15541 caggtgctga tcggcgagac cgctccgtat gcccgtgaca agcgcaccgc gcagcctccg
    15601 ctcaggttcc tgcgcgcgct gctctgcgtg aacggcaact acgcggggac gaagtgcagg
    15661 ctgcgcgccg acggctacgc gcaccacccc tacgagtact cgcacgggcc ggactaccag
    15721 ttcccgggtg gcgacaacgt gacgatcggg acgctcggcc gcctgaccgg cgcgctcgac
    15781 cggcttgccg cgggcggtgc gctgcggacg cccgcgggcg gcgcgctcga cgtctacctg
    15841 accgagttcg gctacttccg cgtcggccgc ttcaagctcc ccgactccac gcgcggcgat
    15901 tacctcaggc gcgccttcga gatcgcgcag gccaacccgc gcgtgcgcca gatgctgcac
    15961 ttcctgctgg tcgagccgcc gaagaagtac gcgttcttcg acacgagcat cgtgtcgcgc
    16021 aagggcaggc cgagcagcgc cttcagcgcg ctggccggct gggcgcaggc cgcggccgcc
    16081 gacgggcgcg tgggcggcta cggcggcggc ggcggtggcg gcggaggttc cggcggtgga
    16141 ggctccggtg gcggtggagg cgaaccgccc ccgcccccaa ccccccc
//
LOCUS       DASVPL010000235        20511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_68034, whole genome shotgun sequence.
ACCESSION   DASVPL010000235 DASVPL010000000
VERSION     DASVPL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 20511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20511
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68034"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6788)
                     /locus_tag="VF517_12615"
     CDS             complement(<1..6788)
                     /locus_tag="VF517_12615"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEX8053828.1"
                     /translation="MALQPSRVRVLIVLSFLLALAGALPTPAAAASPADACAPTGSET
                     VRTDKARYPTEDLVHVSGSGYAAGCEVVVRIVRPDGSVVVGDGSETTGSDVVRTSADG
                     RLAYDYRLGTVSGTYRIYVVHPESGAVLASAEFEDAAILTNLRLGATGAENYVFAAGD
                     RVDAAGTVSNSRNYRWTILRSDGTSTVGPCTATSSTTQGGSEAVTYSYTVAASDPAST
                     ATPYTFRLQEFANNDTTCTGATPSTADLQFTVARATSYSDAALTAPRAAFGAGQSAYL
                     RVDGVTASQADWSVTWIPPSGSVACANTLGTDRPDSSAAGRLPDTAGSFLQYAPGASG
                     AVWNLSANFDAGASCPAFGPSNDGQWRVRLQRDATHSVELPAFLVATTPPQTTIDSGP
                     SGTTTGVSPSFAFSSDKPGTTFRCKLDGPGAATGTYAACSSPRNYTNLAEGSYTLSVY
                     ATDTAGNDDATPATRSFTVDTTLPVVTLTQPLDGSATTDTTPVLAGAAGNAPGDGADV
                     TVNVYAGATASGSPVRTFTVARAGTAWSIVDSNWDAAVALRAPLADGVYTARAEQSDA
                     GGPGQSNTRTFRVDTTAPATTDDVPTTTQPDDVTVTLTALDSGSGVFRTYYTSDDSDP
                     ADPANPSRRTYDGAAKPVLGDGQQIRYYSVDQAGNAEPVRTSRIVKVDRGSTMVEADG
                     NQENGSGTSLTGPASSDWLGFWNSGRVATMVDPNALDDYFTGGTKEQSPLFWGFQNQA
                     GGVTPAKSNVQAAWTSLESTRSTTFLYVAFKREGTTGNTFLTFELNQGTDSYVNSTGT
                     TVPCRQNGDILVSFETGNPPTVTIYRWVSTAPGPPSCPQGAIGTFISSGPLTPAQFQG
                     RMNTAPIVNHLDTGRLGTSFPTNSFGEAGIDLPAVLNQMTGTPCASFARVHVHSRSSE
                     QISSQLQDNVGPYPLNVQSCAVTGTKFEDVDGDGVRDSGEPGIGGFRVYVDLDGDGSL
                     DAGEPSDVSSASGAYEITGVPAGSYDVREALTPAQVAAGWTCSKPVGCRKAIEMTTGG
                     NTTGVEFGNWRPATVSGTKFVDTNRNGFRDGAEGPLAGGTVYVDYNGNGTRDAGEPLA
                     VTGADGRYTIGGIRPGSFTLRQEPLPGYVCTAPSPSCSFPPITFTSGGTVTEKDFADA
                     PSGRVSGTKFEDRNADGDRDGGEPGLASWTVYVDYNGNGSLDAGEPSDVTDGAGDYSI
                     DDVRAPGTHRVREVAQGGWTCSLPSPCSYSIAFVAGTDATGRDFGAWRPGSVSGTVWN
                     DLDEGGTRGGGEPGLGGWTVFVDLDGNGVLDAGEPTAATAADGSYTIAGVRPGSYSVR
                     QVAQGGWRCTAPAGCAHSAVTVDSGAASSGRDFGNAQSTTVSGTKWADHDRDGVQDSN
                     EPGLSGWTVYVDYDGDDALGALEPSAVTDLNGDYTIARIAPNTAANPYAVRELPRANW
                     VCTAPAGDCEWEGLVIAAGGAEVDRDFGAYRVQSVSGRTYEDRDANGAENGGDSARGG
                     VTVWVESIADNGVIDAGEPSAVSDARGYYLIPDVPAGSFKVRQAAAAGWTCSVASADA
                     SGCFRSIMLAPGGTAPGNDFGAWRPASVSGTVFEDIDRDGARGVDEGVLAGATVVVET
                     VTENGVADPGEPTATVGPDGSYSIGGLAPRTTPYRVVALPPAGHTCTAPAACAHSVAL
                     LSGDSAEGRDFGMVGEASVSGVQFDDLNADGVREGGEPGVGGRTVWVDYDNDGVRDAA
                     EPSDVTDGSGAYSIDRVRLGGFRVRQEPATGWTCSAPAGCSHRVDFAAGTDTTDRDFG
                     AWRPGSVSGTVFDDLDRGGDRDGGEPGLSGWTAFVDYDGDEVRDTDEPSAVSSGDGTY
                     TIPGVRPGVWPVREELQGAYTCTVPVPCERSATVTSGGGSAGNDFGNAEPGVTLEGTV
                     FHDRDADGAARELDPVTGEPVEPGLDGRTVWLETVAANGVADPGEPTTVTSALGDYTF
                     RNLNPGSYTVRHDTGSAWNCSYPGGCEWGVSAAGGDSVVGQDFGDWTSPGISGRVYED
                     GNADGGWDAGEPYLNGWRVYLDMNDDGDFDAGSDPYAISATSGGDDGVYSIAGIVPDG
                     KTHEVREEPPSGTWRCTEPANGSVATDCSRSFQNDSEATQAGDFGNYRPVTIAGVKYE
                     DRDADGDRDTGEPGLGGRTIKLDPGTPADPSDDLSTTTAGDGTYSFPGLKPGTTYRVY
                     DEGESGWTCSEPGSPCEYSVPTQSGDGTVERDFGAWRPVTIAGVKYEDRDADGDRDTG
                     EPGLGGRTIKLDPGT"
     gene            6908..7447
                     /locus_tag="VF517_12620"
     CDS             6908..7447
                     /locus_tag="VF517_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053829.1"
                     /translation="MGFRGLFTLAAVLTAALAVPSTALGVRAPERAPLDLATGLTAAP
                     LCTGEQTVSWQPAGAGTFTGTLVGRFDSCPAQGYVVRGVCRLSVLGPDGTAIQTAEGY
                     AYGVTEPTACDPGPLTVGPLPAADTSLTVRFWANFNIRGPSFEGVPTVLCPNTFGPSM
                     TCESNMSLAPGHDQAARFQ"
     gene            7487..8416
                     /locus_tag="VF517_12625"
     CDS             7487..8416
                     /locus_tag="VF517_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX8053830.1"
                     /translation="MSLVVVGSIAFDAVKTPFGERERMLGGSAVHFSLAASFFTDVRV
                     VGPVGEDFGDDAYAVLHGRGVNTGDIERVEGGKTFFWRGRYEFDLNTAHTEDTQLGVF
                     GEFSPKLSDESRQAGTLFLANIQPDIQREVREQCEGARFAGLDSMNLWIDTARESLVR
                     SIGLVDLVFLNDAEIRMLTEEPNLVRAARKVMDMGPRVVIAKQGEYGAALFTEDRFFS
                     LPAYPLETVRDPTGAGDTFAGGFMGYLASVGPDATNDDDELRRAMTYGSVMASFNVED
                     FGTERVSRLEESEIGERYEEFREITRLSEAPAR"
     gene            complement(8413..9807)
                     /locus_tag="VF517_12630"
     CDS             complement(8413..9807)
                     /locus_tag="VF517_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053831.1"
                     /translation="MNKRAPSIAQIAVMVIFALSCFGLLTYIWKSFGGPSPLAPKGYR
                     IQADFDEATQLSSTADVRISGITVGRVKKSELGGDRTRVEMEIERRYAPLPIDTRAIV
                     RQKTLLGESYVELTPGDKRKGVLFDNGRLRRSQVERTTELDEVTRALDLDTRRDLQRF
                     LKAVAALDGEGDDLNAALGQLPRFTRDSTDLLGTLDREHEAVRALVSDGGTVLEALGR
                     RSGELAGLVRSADAVLATTARRDRELAETVRILPTTLAELRPTLEQVRALSLEARPLV
                     RELRPGARALAPALQDASALAPHARLLFGDLDRLIDVGRKGVPSTTRIVRAAHPLFKL
                     LSPTLQEAAPVVDYLGLYKEDIVIQLANFGAALQAKAPVQATGETLYYLRALVPFLPE
                     GLLVASQRVGSNRYNPYFKPRALDRLAQGLESFDCSHTSNPGEQAPNVPCVVQQQVTF
                     QGSNSAYTHVRRLR"
     gene            complement(9804..11204)
                     /locus_tag="VF517_12635"
     CDS             complement(9804..11204)
                     /locus_tag="VF517_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053832.1"
                     /translation="MRTSLRRHVKPYVRHIAALAGMVALSLAVAGYITVHQRLRFPWQ
                     DEVQIFAEFENAQAVTAGQGQTVDVAGVKVGEIGSVEVEDGHALVRLDISDQDAVGPI
                     YSNAKLNLRPKTGLNDMAVQMDPGRPDPALPDRGELKDGDRIPVENTQANVNPDAVLS
                     GLDTDTRRYLQLLLNAGGGGVRGRSADLRAILKASQPALDHAEKVSAAVADRRAKVRR
                     LIHNLRLLSGAAADRDDDLRSLTTAAAAVLRTLGERDVELQGAVQRLPGALAATRAAL
                     VNGRAFAGDARPALDAIRPLARDIAPALVAARPLLREALPVVRDEVRPLVRSATPLLA
                     KLRPSVDRIDQVTEPDLLAVGQTLNRTVNLLGHNPPGPEEGFLFHLAWYVHNATSVLS
                     IQDAQGVAWRGLVMGSCSTFPDVIRATPLFQPLTQLPLCGQPGGQPFPPLVPGPQSIE
                     DLVESATPPRTGEAVR"
     gene            complement(11201..12637)
                     /locus_tag="VF517_12640"
     CDS             complement(11201..12637)
                     /locus_tag="VF517_12640"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053833.1"
                     /translation="MSRGPRSVTTLGAGLMAITIAFVSVYLAFGGPNPYDDKYELKAI
                     VQSGSELHSRTPVRIAGVDVGKVKKVERGPGALATVTMELEQRALPLHRDAQLKIRPR
                     IFLEGNLFVDLTPGTPSAPELKDGGTIPYAQTATPVQLDQVLGALRRSNRDDLAETVR
                     NLGDALDRGGAEALNGALPSMGPAFANGSQAMEALRGQRQHDLSGAIGELATVSGALA
                     DRKEDLADAVSGLARTTAALASRREQLGRSIPELDALLHESRPAFAALNGLFPSARAV
                     VAEARPGVREAPATLRLAHPVLAQLQGLLAPPELPALIRTGTPAIRSLARLQPNLRRL
                     LASVTPVTECARRIVVPTLKKSIDDPPLTTGRPVLPIYRELLNGIVGQSSSTANFDGN
                     GTSLRYHSGFGDESIGTGTVPGAADALAGATSEPLLGSRPRYTGQIPPYRPDVECRTQ
                     DPPDLRAATGPAFSPPGSPRRVIPRQTP"
     gene            complement(12634..14025)
                     /locus_tag="VF517_12645"
     CDS             complement(12634..14025)
                     /locus_tag="VF517_12645"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053834.1"
                     /translation="MRDRRPTSLMGTPILIGSVTVLVLVVAVFLSYNANSGLPFVPTY
                     QISVQVQDAAGLVKGNDVRVGGKRVGSVSQIDGKVGADRPYARLELKLDKPLEPLLDD
                     SQVTVRPRSPLGLKYLEIVPGRSGRPVKAGGELALERSVQTVELDEVLDTFDESTRRA
                     LQRTVSGLGVGVAGRGVDFNESVALAPQLAGNAERVFGNLSDPRTRLRRAVRALGRVT
                     DELGPVAPRAGSLFDNANTTLHALSGVRPELAAVLAGLPPTEATAIDTLRVTRPVLAE
                     ARQLLRDLRPGVRALPSASRRLDAALDRGIPVLRRTGDLAGRLTDALVAVEDLSADPA
                     TRDSLTRLDSVLRSGLPTLQYVVPAQAVCNHLGIWLRNASSTVAEGDLGGTWVRTLII
                     VNHEEMVQTDKPAPRLHFNPYPATAGPGTGGECEAGNEPFLPGQQIGNLPGVQATTTE
                     NTNRNQGLLRTTR"
     gene            complement(14022..15356)
                     /locus_tag="VF517_12650"
     CDS             complement(14022..15356)
                     /locus_tag="VF517_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053835.1"
                     /translation="MIRRLIISALALAGVAALVLFTTAARPGKAVYEVDAIFDNAGFL
                     IEGQDVKIGGAKVGSVKRVALTRDRRARVTMEVEERFGPFRKDARCSIRPQSLIGEKF
                     VQCNPGTPKAPELARHGGARPTVPIARNHSPVDIDLVFAALRRPVRERLSILLNELGT
                     GLAGRPEELNQAIRRANPALGEARQTLEILSGERHTLGRLIDESDRVLGELADRRESV
                     RDFISNANDVTRTMADRRGDIDLAIRRLPPLLDELEPSARELAALTSEARPSVRDLAA
                     SAPSLRRLLADFDPLADATRPTLPKLTELARAGRRAVRAADPVADRLRPVANQLPATV
                     TLARDLTDSLDRQDVAALLGRYVYFAAAAASRFDRFSHILPSYQLAGTCQVYADSTPL
                     RNCDAHFGGYRGDRGVEVPSVSPKRQAAPASKPAPGPARAAEDQALDFLMGP"
     gene            complement(15353..16708)
                     /locus_tag="VF517_12655"
     CDS             complement(15353..16708)
                     /locus_tag="VF517_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053836.1"
                     /translation="MPVGRRTHVLARLLTLAALLGAIAVIAWLLFASDDSYRVTLTLD
                     NASQLVKGNQVKVGGVPVGTVSKLELGDDAQALVELDITDEELQPLHRGTTVEVRSTS
                     LSGIANRYVALKPGPNDEEPIEDGGAIAATDAESEVDLDAVLNSLSPEVLGDLQATVH
                     GLGAATDGRGKELEDAMVALNPALSQTASTAREVMRDEGAFSRFLVDSASVVSAVAER
                     RPDLERLVPATGATLSAIAERTASLDSSLRQLPPTLREANTTLVNLRALVGDLRPLVR
                     EARPVAPLLTETLTRLRPVARSGVRVVPALRRLIDRPGREDLIGVLAEMPRLEDAAVP
                     AFESTVATVKDALPVVRELRPYTPDFVGAYRAYGGTNSAYYDANGHYQRIAFVGSGFT
                     LSPGTGNVVPLLPEQGGLAGYQTFRRNRCPGAAAQTHPDRSNPWQPEAGFPCDPEQAV
                     R"
     gene            complement(16771..17601)
                     /locus_tag="VF517_12660"
     CDS             complement(16771..17601)
                     /locus_tag="VF517_12660"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX8053837.1"
                     /translation="MSDPGLFGPDSVTWRVNREGVLLAGGGTALILQLAHPLVAAGVS
                     QHSNYREDPWGRLYRTLDLTTKIQFGSTATAEEASARIRHTHGRVRGKSDQAAGAYAE
                     GTPYDARTHDLIMWVHATLVHTSLLVYQRYVRPLTIAEQRRYYEEQKLLGEKFGVPRE
                     HQPATYADFNDYFEAMLASERIAPTEALLDVVDAVMRPELPFVARPIVEALNLSTVGH
                     LPTSLRDELGLPWGPHRARLFEASRLVLSAALPVLPKLLREFPPARSADRRVRRLAAA
                     "
     gene            17656..17916
                     /locus_tag="VF517_12665"
     CDS             17656..17916
                     /locus_tag="VF517_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053838.1"
                     /translation="MGWGFVWMMVVLKLPIVALLWLVWWAIRQTDAEAELPTDDGGSD
                     RHPHRGGPRRPRPPRRGPHAEPAPRAPSRIRVAGRQLTRPLR"
     gene            17928..18209
                     /locus_tag="VF517_12670"
     CDS             17928..18209
                     /locus_tag="VF517_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053839.1"
                     /translation="MSLPLAEFNAYHGIGIAFAVWAVILSLLGMRSADFPRSGGIQRA
                     VVAISALLLVSAVGAAIATSEKHGAHESSHGDRENPTHPGESPENVPNE"
     gene            18305..19639
                     /locus_tag="VF517_12675"
     CDS             18305..19639
                     /locus_tag="VF517_12675"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX8053840.1"
                     /translation="MAEEQTQSQDGGGVATAEQTLTVTDNRTGETYEVEVSDGTVKAM
                     DFRQIKVDEDDFGLMTYDPAFTNTAACRSEITYIDGEAGVLEHRGYKIEELCERASYL
                     EVAYLLIHGELPNQSQLDDWTHEITIHTFVHENIKRFMEGFRYDAHPMGMLLASVGAL
                     STFYPGAKDIQDHSERHMAAVRLIAKVPTLAAFAYRHSLGLPYNYPDNDLDYPSNFLQ
                     MMFRMTELKYEPDPRLAKALDVLWILHADHEQNCSTNAVRAVGSSKVDPYSAVAAGVA
                     ALYGPLHGGANEAVLKMLDRIGTKENIPDFLEGVKEGKERLMGFGHRVYKNYDPRARI
                     IKKHVDEVFEVTGVNPKLELAVELEKRALDDEYFTDRKLYPNVDFYSGLIYEALTLPP
                     EMFTVMFAIPRTSGWIAQWIEMIDDKEQKIARPRQIYTGEREREFVPVADRG"
     gene            19692..19892
                     /locus_tag="VF517_12680"
     CDS             19692..19892
                     /locus_tag="VF517_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053841.1"
                     /translation="MKKLVVVAVTAMALVAAAPASAEPGKDQGCGDIFRGLGAPGFGQ
                     AVSEVAGETGFDDYVQVVCRHF"
     gene            complement(19889..20338)
                     /locus_tag="VF517_12685"
     CDS             complement(19889..20338)
                     /locus_tag="VF517_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053842.1"
                     /translation="MPGRAYPLPVAAAVCFVATAGDRAAVAAKSRADVQSPSSSPGAL
                     DGGTPNGPSTNAVISQDRRWSRYVAFESEASDLTRGDTNGAKDVFVVRRDRRRVLVGA
                     VVRRLPLVCQERSLRSSQYEDPVPPAAPPQRPPPENRQVYMRYLGPV"
BASE COUNT         2676 a         7277 c         7677 g         2881 t
ORIGIN      
        1 gtgccggggt cgagcttgat cgtgcgcccg ccgaggccgg gctcgccggt gtcgcgatcg
       61 ccgtcggcgt cgcggtcctc gtacttgacg ccggcgatcg tgaccggccg ccaggcgccg
      121 aagtcgcgct cgaccgtgcc gtcgccggac tgcgtcggga cgctgtactc gcagggactg
      181 cccggctcgg agcaggtcca gccgctctcg ccctcgtcgt agacgcggta ggtcgtgccg
      241 ggcttgaggc cggggaagct gtaggtgccg tcgccggcgg tggtggtcga gaggtcgtcg
      301 ctcgggtcgg ccggggtgcc ggggtcgagc ttgatcgtgc gcccgccgag gccgggctcg
      361 ccggtgtcgc gatcgccgtc ggcgtcgcgg tcctcgtact tgacgccggc gatcgtgacc
      421 gggcggtagt tgccgaagtc gcccgcctgc gtggcctccg agtcgttctg gaacgagcgc
      481 gagcagtcgg tggcaaccga gccgttcgcg ggctcggtgc agcgccacgt gccgctcggc
      541 ggctcctcgc gcacctcgtg cgtcttgccg tcgggcacga tgccggcgat ggagtacacg
      601 ccgtcgtcgc cacccgaggt cgcgctgatc gcgtacgggt cgctgcccgc gtcgaagtcg
      661 ccgtcgtcgt tcatgtcgag gtacacacgc cagccgttca ggtacggctc gcccgcgtcc
      721 cagccgccgt cggcgttccc gtcctcgtag acgcggcccg agatgccggg gctcgtccag
      781 tcgccgaagt cctggcccac gacgctgtcg ccgccggcag cgctcacgcc ccactcgcag
      841 ccgccggggt aggagcagtt ccaggccgag ccggtgtcgt gacgcaccgt gtagctgccg
      901 gggttgaggt tgcggaaggt gtagtcgcca agcgcgctcg tgacggtcgt cggctcaccc
      961 gggtcggcca cgccgttcgc cgcgaccgtc tcgagccaca cggtccgccc gtcgaggccc
     1021 ggctcgaccg gctcgccggt gacgggatcg agctcgcgcg ctgcgccgtc tgcgtcgcgg
     1081 tcgtggaaca cggtgccctc gagcgtcacg cccggctccg cgttgccgaa gtcgttgccc
     1141 gccgagccgc cgcccgaggt cacggtcgcg ctgcgctcgc acggcaccgg cacggtgcac
     1201 gtgtacgcgc cctggagctc ctcccgcacg ggccacacgc ccggccgcac gccggggatc
     1261 gtgtaggtgc cgtcgcccga gctgacggca gacggctcgt cggtgtcgcg cacctcgtcg
     1321 ccgtcgtagt ccacgaacgc ggtccaaccg gagagcccgg gctcgccgcc gtcgcggtcg
     1381 ccgccacggt cgaggtcgtc gaacacggtg ccggacacgc tgccggggcg ccaggcgccg
     1441 aagtcgcgat cggtcgtgtc ggtgcccgcc gcgaagtcca cgcggtggga gcacccggcc
     1501 ggggccgagc aggtccaccc cgtcgccggc tcctgccgga cgcggaagcc gccgaggcgc
     1561 acccggtcga tcgagtaggc gccgctgccg tcggtgacgt cggagggctc ggccgcgtcg
     1621 cggacaccgt cgttgtcgta gtccacccac accgtgcggc cgccgacgcc gggctcgccg
     1681 ccctcgcgca cgccgtccgc gttcagatcg tcgaactgca cgcccgacac cgacgcctcg
     1741 ccgaccattc cgaagtcgcg gccctcggcg gagtcgccgg agaggagtgc cacggagtgc
     1801 gcgcaggcgg ccggcgccgt gcaggtgtgg ccggccggcg gcagcgccac gacgcggtac
     1861 ggcgtggtgc gcggcgccag cccgccgatc gagtaggagc cgtcggggcc gaccgtcgcg
     1921 gtcggctcgc cggggtcggc cacgccgttc tcggtcacgg tctcgacgac cacggtcgca
     1981 cccgccagca cgccctcgtc gacgccgcgc gcgccgtcgc gatcgatgtc ctcgaagacg
     2041 gtgccgctca cgctcgcggg gcgccacgcg ccgaagtcgt tgcccggtgc ggtccccccg
     2101 ggagcgagca tgatcgaccg gaagcagccg ctggcgtcgg cgctcgcgac cgagcaggtc
     2161 cagccggcgg ccgcggcctg gcgcaccttg aacgacccgg cgggcacgtc cgggatcagg
     2221 tagtagccgc gcgcgtcgct gacggcgctc ggctcgccgg cgtcgatcac gccgttgtcg
     2281 gcgatcgact cgacccagac cgtcacgccg ccgcgggcgg agtcgccgcc gttctcggcg
     2341 ccgttcgcgt cgcgatcctc gtaggtgcgc ccgctcaccg actgcacccg gtacgcgccg
     2401 aagtcgcggt cgacctcggc gccgcccgcg gcgatcacca ggccctccca ctcgcagtcg
     2461 ccagccggtg cggtgcagac ccagttcgcc cgcggcagct cgcgcaccgc gtacgggttg
     2521 gccgcggtgt tcggcgctat ccgcgcgatc gtgtagtcgc cgttcaggtc ggtgaccgcg
     2581 ctgggctcga gagcaccgag cgcgtcgtcg ccgtcgtagt ccacgtagac ggtccagccc
     2641 gagaggcccg gctcgttcga gtcctgcacc ccgtcgcggt cgtggtcggc ccacttggtg
     2701 ccggagacgg tggtgctctg agcgttgccg aagtcgcgcc cgctgctcgc cgcgcccgag
     2761 tcgacggtca cggccgagtg cgcgcagccc gccggcgccg tgcagcgcca gccgccctgc
     2821 gccacctggc ggaccgagta cgagccgggc cgcacgccgg cgatcgtgta gctgccgtcg
     2881 gccgccgtcg cggcggtcgg ctcgcccgcg tcgagcacgc cgttgccgtc gaggtcgacg
     2941 aagacggtcc agccaccgag gccgggctcg ccgccaccgc gcgtgccgcc ctcgtcgagg
     3001 tcgttccaga cggtgccgct gacggagccg ggccgccagg cgccgaagtc gcgcccggtc
     3061 gcgtcggtgc cggcgacgaa cgcgatcgag taggagcacg ggctcgggag cgagcaggtc
     3121 cagccgccct gggcgacctc gcggacgcgg tgcgtgccgg gcgcgcggac gtcgtcgatc
     3181 gagtagtccc cggcgccgtc ggtcacgtcg gacggctcac ccgcgtcgag gctcccgttg
     3241 ccgttgtagt cgacgtagac ggtccagctg gccaggccgg gctctccgcc gtcgcggtcg
     3301 ccgtccgcgt tgcgatcctc gaacttggtc ccgctgacgc ggcccgacgg tgcgtcggcg
     3361 aagtccttct cggtgaccgt cccaccggag gtgaaggtga tcggcgggaa cgagcagctc
     3421 gggctcggcg cggtgcagac gtaacccggc agcggctcct ggcgcagcgt gaagctgccc
     3481 gggcggatgc cgccgatcgt gtagcggccg tcggcgcccg tgaccgcgag cggctcgccc
     3541 gcgtcgcgcg tcccgttgcc gttgtagtcg acgtagacgg tgccgccggc gagcggcccc
     3601 tcggcgccgt cgcggaagcc gttgcggttc gtgtccacga acttcgtccc gctgacggtc
     3661 gcgggccgcc agttgccgaa ctcgacgccg gtcgtgttgc cgcccgtcgt catctcgatc
     3721 gccttgcggc agccgaccgg cttcgagcac gtccagccgg cggccacctg ggccggggtg
     3781 agcgcctcgc gcacgtcgta ggaccccgcg gggacgcccg tgatctcgta cgcgccgctc
     3841 gcgctgctca cgtccgaggg ctcgcccgcg tcgagcgacc cgtcgccgtc gagatccacg
     3901 tacacgcgga acccgccgat ccccggctcg ccggagtcgc gcacgccgtc cccgtccacg
     3961 tcctcgaact tcgtcccggt caccgcgcag ctctgcacgt tcagcgggta cggtccgacg
     4021 ttgtcctgga gctgggagct gatctgctcg gacgagcggc tgtgcacgtg cacccgcgcg
     4081 aacgaggcgc acggcgtgcc ggtcatctgg ttgaggacgg ccgggaggtc gatgcccgcc
     4141 tcgccgaacg agttggtcgg gaagctcgtc ccaagccggc ccgtgtcgag gtggttcacg
     4201 atcggcgccg tgttcatccg gccctggaac tgggcgggcg tgagcggccc gctcgagatg
     4261 aacgttccga tcgcgccctg cgggcacgac ggcggccccg gcgcggtgct cacccagcgg
     4321 tagatcgtca cggtcggcgg gttgccggtc tcgaagctca cgaggatgtc gccgttctgg
     4381 cggcagggca cggtcgtgcc agtggagttg acgtacgagt cggtgccctg gttcagctcg
     4441 aacgtgagga aggtgttgcc ggtcgtcccc tcgcgcttga acgcgacgta caggaaggtg
     4501 gtcgagcggg tggactcgag ggaggtccac gccgcctgca cgttcgactt cgcgggcgtg
     4561 acgccgccgg cctggttctg gaagccccag aacagcggcg actgctcctt ggtccccccg
     4621 gtgaagtagt cgtcgagcgc gttcgggtcg accatcgtcg ccacgcgtcc ggagttccag
     4681 aagccgagcc agtcgctgct cgccgggccg gtgagcgagg tgccggaccc gttctcctgg
     4741 ttgccgtcgg cctccaccat cgtgctgccg cggtcgacct tcacgatgcg cgaggtgcgc
     4801 accggctcgg cgttgccggc ctggtccacc gagtagtagc ggatctgctg gccgtcgccg
     4861 agcacgggct tggcggcgcc gtcgtaggtg cgccgggacg ggttcgccgg gtcggccggg
     4921 tccgagtcgt cggacgtgta gtaggtgcgg aagacgcccg agcccgagtc gagcgcggtg
     4981 agcgtgaccg tgacgtcgtc gggctgcgtg gtcgtgggga cgtcgtcggt cgtcgccggg
     5041 gcggtggtgt cgacgcggaa ggtgcgcgtg ttgctctgac cggggccgcc cgcgtccgac
     5101 tgctcggcgc gagcggtgta gacgccgtcg gccagcggcg cacgcagcgc caccgcggcg
     5161 tcccagttcg agtcgacgat cgaccacgcg gtcccggcgc gcgcgacggt gaaggtgcgc
     5221 accggcgagc cggaggcggt cgcgccggcg tagacgttca cggtcacgtc cgcgccgtcg
     5281 ccgggcgcgt tgcccgcggc acctgcgaga acgggggtcg tgtcggtcgt cgcggagccg
     5341 tcgagcggct gcgtcagggt gacgaccggg agcgtcgtgt ccacggtgaa agagcgcgtg
     5401 gccggcgtgg cgtcgtcgtt gcccgccgtg tccgtggcgt agacgctgag ggtgtagctg
     5461 ccctcggcga ggttcgtgta gttgcgcggc gagctgcagg cggcgtacgt gccggtggcc
     5521 gcgccgggtc cgtcgagctt gcagcggaag gtggtcccag gcttgtcgga gctgaaggcg
     5581 aagctcgggg agacgccggt cgtcgtgccg ctcgggccgc tgtcgatcgt cgtctgcggc
     5641 ggggtggtcg cgacgaggaa tgccggcagc tcgaccgagt gggtcgcgtc gcgctggagc
     5701 cgcacgcgcc actggccgtc gttggagggg ccgaatgccg ggcagctcgc cccggcgtcg
     5761 aagttcgcgg acaggttcca cacggccccg ctcgcgccgg gcgcgtactg gaggaagctg
     5821 ccggccgtgt cgggcaggcg cccggcggcc gaggagtcgg ggcggtcggt cccgagcgtg
     5881 ttcgcgcagg ccaccgagcc cgagggcggg atccaggtga cgctccagtc ggcctgcgac
     5941 gcggtgacac cgtcaacccg caggtacgcg ctctgcccgg caccgaacgc ggcgcgcggc
     6001 gccgtgagcg cggcgtcgga gtacgaggtc gcccgcgcca cggtgaactg gaggtccgcg
     6061 gtgctcgggg tcgcgcccgt gcaggtcgtg tcgttgttcg cgaactcctg gagccggaag
     6121 gtgtagggcg tggccgtcga ggcggggtcc gaggcggcca ccgtgtacga gtacgtgacg
     6181 gcctcgctgc cgccctgcgt cgtggagctg gtggccgtgc acgggccgac cgtcgacgtc
     6241 ccgtccgagc ggaggatcgt ccagcggtag ttgcggctgt tcgagaccgt gcccgcggcg
     6301 tcgacccggt cccccgcggc gaagacgtag ttctcggcgc ccgtcgcgcc gaggcgcagg
     6361 ttggtgagga tcgcggcgtc ctcgaactcg gcgctcgcca ggaccgcgcc gctctcgggg
     6421 tggaccacgt agatccggta ggtcccggag accgtgccga gccggtagtc gtaggccagc
     6481 cggccgtccg cactcgtgcg cacgacgtcg ctgccggtcg tctcgctccc gtcgccgacg
     6541 accacgctgc cgtcgggccg gacgatccgg acgacgacct cgcagcccgc ggcgtagccg
     6601 gagccgctca catgcacgag gtcctcggtc gggtagcgcg ccttgtcggt ccggacggtc
     6661 tcggagcccg tgggggcgca ggcgtcggcc ggcgaagcgg ccgcggcggg cgtggggaga
     6721 gcacccgcga gcgcgagcag gaacgagagg acgatcagca cgcggacgcg cgaaggctgg
     6781 agggccatga gtcgccatcg gcaagcggcg ccgcgcgctt tatcggccgg gagggggatt
     6841 gcgagggcgg cgcgcaccac gggcgcgcga gttaccctcc ggcgagcaac ctacgcgggg
     6901 gggacgcatg gggtttcggg ggctcttcac gttggcggcc gtcctgacgg ccgccctggc
     6961 tgtgccgagc actgcgctcg gtgttcgcgc gccggagcgc gcaccgctcg accttgcgac
     7021 cggcctgacg gccgcgccgc tgtgcacggg tgagcagacc gtgagctggc agccggccgg
     7081 cgccggcacg ttcacgggca cgctggtcgg tcggttcgac agctgcccgg cgcaggggta
     7141 cgtggtgcgc ggcgtctgcc ggctgagcgt gctcggcccc gacggcacgg cgatccagac
     7201 cgccgagggc tacgcctacg gcgtcacgga gcccaccgcc tgcgaccccg gcccactcac
     7261 ggtcggaccg ctccccgccg ccgacaccag cctcacggtg cgcttctggg cgaacttcaa
     7321 catccgcggc ccgagcttcg agggggtccc gacggtgctc tgcccgaaca cgttcggccc
     7381 atccatgacg tgcgagagca acatgagcct ggcgcccggt cacgaccagg ccgcccgctt
     7441 ccagtagccg gggaggcgcc ccggcgtcgc tagggttgcg ccgcctatga gcctggtggt
     7501 ggttggatcg atcgcgttcg acgcggtgaa gacgcccttc ggggagcgcg agcgcatgct
     7561 cggcggctcc gccgtgcact tctcgctcgc cgcgagcttc ttcacggacg tgcgcgtggt
     7621 cggcccggtc ggcgaggact tcggcgacga cgcctacgcc gtcctccacg gccgcggggt
     7681 gaacaccggc gacatcgagc gcgtcgaggg cggaaagacc ttcttctggc gcgggcgcta
     7741 cgagttcgac ctcaacaccg cgcacaccga ggacacgcag ctcggcgtgt tcggcgagtt
     7801 ctcgccgaag ctctcggacg agtcgcggca ggccggcacg ctgttcctcg ccaacatcca
     7861 gccggacatc cagcgcgagg tccgcgagca gtgcgagggc gcgcgcttcg ccgggctcga
     7921 ctcgatgaac ctctggatcg acaccgcgcg cgagtcgctc gtgcgctcga tcggcctcgt
     7981 ggacctcgtg ttcctcaacg acgccgagat ccgcatgctc accgaggagc cgaacctcgt
     8041 gcgcgcggcg cgcaaggtga tggacatggg cccgcgcgtg gtgatcgcca agcagggcga
     8101 gtacggcgcc gcgctgttca ccgaggaccg cttcttctcg ctcccggcct acccgctgga
     8161 gaccgtgcgc gaccccaccg gcgccggcga caccttcgcc ggcggtttca tgggctacct
     8221 cgcgtcggtc ggaccggacg cgacaaacga cgacgacgag ctgcgccgcg cgatgaccta
     8281 cggctcggtg atggcgtcgt tcaacgtcga ggacttcggc accgagcgcg tgtcacgcct
     8341 cgaggagagc gagatcggcg agcgctacga ggagttccgc gagatcacgc gcctgtcgga
     8401 ggcgccggcc cgctagcgca ggcgccgcac gtgcgtgtac gcgctgttgc tgccctggaa
     8461 ggtcacctgc tgctggacga cgcagggcac gttcggcgcc tgctcgccgg ggttcgacgt
     8521 gtgcgagcag tcgaacgatt cgaggccctg cgccaggcgg tcgagcgcgc gcggcttgaa
     8581 gtacgggttg tagcggttcg agccaacccg ctggctggcg accagcagcc cctccggtag
     8641 gaacggcacc agcgcgcgca ggtagtagag cgtctcgccg gtcgcctgca ccggcgcctt
     8701 ggcctggagc gccgcgccga agttggcgag ctggatgacg atgtcctcct tgtagagccc
     8761 gaggtagtcg accaccggcg ccgcctcctg gagcgtcggc gacagcagct tgaagagcgg
     8821 gtgtgccgcg cgcacgatcc gcgttgtcga gggaacgccc ttgcgaccca cgtcgatcag
     8881 gcggtcgagg tcgccgaaca gcaggcgcgc gtgcggcgcg agcgccgacg cgtcctggag
     8941 ggccggcgcg agcgcgcggg cgcccggccg cagctcgcgg acgagcgggc gcgcctcgag
     9001 cgacagcgcc ctgacctgct cgagcgtcgg gcgcagctcc gcgagcgtcg taggcaggat
     9061 gcgcaccgtc tcggccagct cgcggtcgcg ccgcgcggtg gtggcgagca cggcgtccgc
     9121 cgagcgcacg agccccgcca gctcgcccga ccggcggccg agcgcctcga gcaccgtccc
     9181 gccgtcgctc acgagcgcgc ggaccgcctc gtgctcgcgg tcgagcgtgc cgagcaggtc
     9241 ggtcgagtcg cgtgtgaaac gcgggagctg cccgagcgcc gcgttcaagt cgtcgccctc
     9301 tccgtcgagc gccgcgaccg ccttcaggaa ccgctggagg tcacggcgcg tgtcgaggtc
     9361 gagcgcgcgc gtgacctcgt cgagctcggt cgtccgctcg acctgcgagc gccgcagccg
     9421 gccgttgtcg aacagcaccc ccttgcgctt gtcgccgggt gtcagctcga cgtagctctc
     9481 gcccagcagc gtcttctggc ggacgatcgc gcgcgtgtcg atcggcagcg gcgcgtagcg
     9541 gcgctcgatc tccatctcga cccgggtgcg gtcgccgccg agctcggact tcttcacccg
     9601 tccgaccgtg atgccggaga tgcgcacgtc ggcggtgctc gagagctgcg tggcctcgtc
     9661 gaagtcggcc tggatccggt agcccttcgg cgcgagcggc gagggcccgc cgaacgactt
     9721 ccagatgtat gtcaacaggc cgaagcagga cagcgcgaag atcaccatca cggcgatctg
     9781 ggcgatgctc ggcgcgcgct tgttcacctc accgcctccc cggtcctcgg tggggtcgcg
     9841 ctctcgacca ggtcctcgat cgactgcggg cccggcacca gcggtgggaa cggctgcccg
     9901 ccgggctgac cgcagagcgg gagctgcgtt agcggctgga acagcggcgt cgcgcggatc
     9961 acgtcgggga acgtcgagca gctccccatc acgagcccgc gccaggcgac gccctgcgcg
    10021 tcctggatcg acagcaccga cgtcgcgttg tgcacgtacc aggcgaggtg gaagaggaag
    10081 ccctcctccg ggccgggcgg gttgtgcccg agcaggttga ccgtgcggtt cagggtctgc
    10141 ccgaccgcga gcaggtccgg ctcggtgacc tggtcgatgc ggtcgaccga cgggcgcagc
    10201 ttcgccagga gcggcgtggc cgagcgcacc agcgggcgga cctcgtcgcg cacgaccggc
    10261 agggcttcgc gcaggagcgg ccgcgcggcc acgagcgccg gggcgatgtc gcgcgcgagc
    10321 ggccggatcg cgtcgagcgc cgggcgggcg tcgccggcga acgcgcgtcc gttcaccagc
    10381 gccgcgcgcg tcgcggccag cgcgccgggc agtcgctgca ccgcgccctg gagctccacg
    10441 tcgcgctcgc cgagcgtgcg cagcacggcg gcagccgctg tcgtcaggct gcgcaggtcg
    10501 tcgtcgcggt cggcagccgc gccgctgagc agccgcaggt tgtggatcag ccgcctgacc
    10561 ttcgcgcggc ggtccgccac ggcggccgag accttctccg cgtggtcgag cgccggctgc
    10621 gacgccttca ggatcgcccg caggtccgcg ctgcggccgc gcacgccgcc gccgcccgcg
    10681 ttcagcagga gctggaggta gcgccgcgtg tcggtgtcga gcccggacag gaccgcgtcc
    10741 gggttcacgt tcgcctgtgt gttctcgacc ggtatccggt cgccgtcctt cagctcgccg
    10801 cggtccggca gggccgggtc gggccggccc gggtccatct gcactgccat gtcgttcagc
    10861 cccgtcttcg gccgcaggtt gagcttggcg ttcgagtaga tcgggccgac cgcgtcctgg
    10921 tcgctgatgt cgagccggac gagcgcgtgg ccgtcctcca cctcgaccga gccgatctcg
    10981 ccgaccttca ccccggccac gtccacggtc tggccctgac cggcggtgac ggcctgcgcg
    11041 ttctcgaact ccgcgaagat ctggacctcg tcctgccagg ggaagcgcag gcgctggtgg
    11101 accgtgatgt acccggccac cgccagcgag agcgcgacca tccccgcgag cgcggcgatg
    11161 tggcggacgt acggcttgac gtgccggcgc agcgacgtcc tcatggcgtc tgcctcggga
    11221 tcactcgccg cgggctgccg ggcgggctga acgccggacc ggtcgcggcc ctgaggtcgg
    11281 gcgggtcctg cgtgcggcac tcgacgtccg gccggtaggg cgggatctgc cccgtgtagc
    11341 gcgggcgcga cccgagcagc ggctcggagg tggccccggc cagcgcgtcg gccgcgcccg
    11401 gcaccgtgcc ggtgccgatg ctctcgtccc cgaagcccga gtggtagcgc agcgaggtgc
    11461 cgttgccgtc gaagttggcc gtggacgagc tctggccgac gatgccgttg agcagctcgc
    11521 ggtagatcgg caggaccggt cgcccggtgg tgagcggcgg gtcgtcgatc gacttcttca
    11581 gcgtcggcac gacgatgcgg cgcgcgcact cggtgacggg cgtgacggag gcgagcagcc
    11641 ggcgcaggtt cggctggagc cgggcgagcg agcggatcgc gggcgtgccg gtgcggatca
    11701 gcgccggcag ctccggcggc gcgagcagcc cctggagctg ggccagcacc gggtgggcga
    11761 gccgcagcgt ggcgggcgcc tcgcgaacgc cgggacgcgc ctccgccacc accgcgcgtg
    11821 cgctcgggaa gagcccgttg agcgcggcga aggcggggcg ggactcgtgc agcagcgcgt
    11881 cgagctcggg tatcgaccgg ccgagctgct cgcgccgcga cgccagggcg gcggtggtgc
    11941 gcgcgagccc gctgaccgcg tccgcgaggt cctccttgcg gtccgccagc gcgcccgaca
    12001 ccgtcgccag ctcgccgatc gcccccgaca ggtcgtgctg gcgctggccg cgcagcgcct
    12061 ccatcgcctg cgagccattc gcgaacgcgg ggcccatcga ggggagcgcg ccgttcagcg
    12121 cctccgcgcc gccgcggtcg agcgcgtcgc cgaggttgcg cacggtctcg gcgaggtcgt
    12181 cccggttcga gcgccgcagc gcgccgagca cctggtcgag ctggaccggc gtggccgtct
    12241 gcgcgtacgg gatcgtgccg ccgtccttca gctcgggcgc cgacggcgtg cccggcgtca
    12301 ggtccacgaa gaggttgccc tcgaggaaga tccgcgggcg gatcttgagc tgagcgtcgc
    12361 ggtgcagcgg cagcgcgcgc tgctccagct ccatggtcac ggtggcgagc gcgcccggtc
    12421 cgcgctcgac cttcttcacc ttgccgacgt cgacgcccgc gatccggacc ggcgtgcgcg
    12481 agtgcagctc gctgcccgac tgcacgatcg ccttcagctc gtacttgtcg tcatatgggt
    12541 tcggcccgcc gaacgcgagg tagaccgaga cgaacgcgat cgtgatcgcc atcaggccgg
    12601 cgccgagcgt ggttacggag cgcgggccgc ggctcatcgc gtggtcctca gcaggccctg
    12661 gttgcggttg gtgttctcgg tcgtcgtggc ctggacgccg gggaggttcc cgatctgctg
    12721 gcccgggagg aacggctcgt tgcccgcctc gcactcgccg cccgtgccgg ggcccgcggt
    12781 ggccgggtac gggttgaagt ggagccgcgg ggccggcttg tcggtctgga ccatctcctc
    12841 gtggttgacg atgatcagcg tgcggaccca ggtgccgccg aggtcgccct ccgcgacggt
    12901 cgaggacgcg ttgcgcagcc agatcccgag gtggttgcag accgcctgcg cggggacgac
    12961 gtactggagc gtcggaagcc cgctgcgaag cacggaatcg agccgcgtca gcgagtcgcg
    13021 cgtggccgga tcggcggaca ggtcctcgac cgcgacgagc gcgtcggtga gccggccggc
    13081 caggtcgccg gtccgccgca gcacggggat gccgcggtcg agcgccgcgt ccagccgccg
    13141 cgaggcgctc ggcagcgcgc gcacgcccgg ccgcaggtcg cgcaggagct ggcgcgcctc
    13201 ggcgagcacg ggccgggtca cccgcagcgt gtcgatcgcg gtggcctcgg tcggcggcag
    13261 cccggccagc acggccgcca gctcgggccg cacgccggac agcgcgtgca gcgtggtgtt
    13321 cgcgttgtcg aacaagctgc ccgcgcgcgg cgccaccggc ccgagctcgt cggtcacgcg
    13381 accgagcgcc cgcaccgcgc gccgcagccg cgtgcgcggg tccgagaggt tgccgaacac
    13441 gcgctccgcg ttgcccgcga gttgcggtgc gagggcgacc gactcgttga agtccacacc
    13501 ccggcccgcg acgccgacgc ccagcccgct caccgtgcgc tggagcgccc tgcgcgtgga
    13561 ctcgtcgaag gtgtcgagca cctcgtcgag ctcgacggtc tgcaccgacc gctcgagcgc
    13621 cagctcgccg cccgccttga ccggccgccc ggaccggccg ggcacgatct cgaggtactt
    13681 gagcccgagc ggcgagcgcg ggcgcacggt cacctgcgag tcgtcgagca gcggctcgag
    13741 cggcttgtcg agcttcagct cgaggcgcgc gtacggccgg tcggccccca ccttgccgtc
    13801 gatctggctg acggagccga cccgcttgcc gcccacccgc acgtcgttgc ccttcacgag
    13861 cccggccgcg tcctggacct gcacgctgat ctggtaggtc ggcacgaacg gcagcccgga
    13921 gttcgcgttg taggagagga acaccgcgac cacgagcacg agcacggtca ccgagccgat
    13981 caggatcggc gtgcccatca ggctcgtcgg gcggcgatcc ctcatggccc catcaggaag
    14041 tcgagggcct ggtcctcggc ggcccgggcc ggcccgggcg ctggcttgga cgcgggcgcc
    14101 gcctggcgct tggggctcac cgacgggacc tccaccccgc ggtcgcccct gtagccgccg
    14161 aagtgcgcgt cgcagttgcg cagcggcgtg ctgtcggcgt agacctggca ggtcccggcg
    14221 agctggtacg acggcaggat gtgggagaag cggtcgaagc gcgacgccgc ggccgcggcg
    14281 aagtagacgt agcggcccag cagcgcggcc acgtcctggc gatcgaggga gtccgtgagg
    14341 tcgcgtgcga gcgtgaccgt cgccggtagc tgattcgcca ccggacgcag gcgatcggcc
    14401 accgggtcgg ccgcgcgcac ggcccgccgt ccggctcgcg ccagctcggt gagcttgggg
    14461 agcgtgggcc gcgtggcgtc ggcgagcggg tcgaagtcgg cgagcagccg gcgcagcgac
    14521 ggcgcggacg ccgcgaggtc gcgcacgctc gggcgcgcct cggaggtcag cgcggccagc
    14581 tcgcgcgcgg acggctcgag ctcgtcgagc agcggcggca gccgccggat ggcgaggtcg
    14641 atgtcgccgc ggcggtcggc catcgtgcgc gtcacgtcgt tcgcgttcga gatgaagtcg
    14701 cgcaccgact cgcgccggtc cgccagctcg cccagcaccc ggtcggactc gtcgatcagg
    14761 cgcccgagcg tgtgccgctc gccgctcagg atctcgagcg tctggcgcgc ctcgccgagc
    14821 gcggggttcg cgcgccggat ggcctggttg agctcctcgg ggcggccggc cagccccgtg
    14881 ccgagctcgt tgagcaggat cgagagccgc tcccggaccg gccgccgcag cgcggcgaac
    14941 accaggtcga tgtccaccgg cgagtggttg cgcgcgatcg ggaccgtcgg tcgcgcgccg
    15001 ccgtggcgcg ccagctcggg cgccttcggc gtaccggggt tgcactggac gaacttctcc
    15061 ccgatcaggc tctgcggccg gatcgagcag cgcgcgtcct tgcggaacgg cccgaagcgc
    15121 tcctcgacct ccatcgtcac ccgcgcgcgg cgatcgcgcg tgagcgccac gcgcttgacg
    15181 gagccgacct tcgcgccgcc gatcttcacg tcctggccct ctatcaggaa gccggcattg
    15241 tcgaagatcg cgtcgacctc gtacacggcc ttgccgggcc gcgcggcggt ggtgaagagg
    15301 accagcgcgg cgacgccggc cagcgcgagc gcggagatga tgaggcggcg gatcaccgca
    15361 ccgcctgctc cgggtcgcac gggaagccgg cctccggctg ccacgggttc gaccggtcgg
    15421 gatgcgtctg cgccgcggca ccggggcagc ggttgcgcct gaacgtctgg tagccggcga
    15481 ggccgccctg ctccggcagc agcggcacga cgttgccggt ccccggcgag agggtgaagc
    15541 cgctgccgac gaacgcgatg cgctggtagt ggccgttggc gtcgtagtag gccgagttcg
    15601 tcccgccgta ggcgcggtag gcgcccacga agtcgggcgt gtagggtcgc agctccctga
    15661 caaccggcag cgcgtccttc acggtcgcga cggtggactc gaacgcgggc accgccgcgt
    15721 cctcgaggcg cggcatctcc gccagcacgc cgatcaggtc ctcgcggccg gggcggtcga
    15781 tcagccgccg cagcgccggc accacgcgca cgccgctgcg cgcgacgggc cgcagccgcg
    15841 tgagcgtctc ggtgagcagc ggggccaccg ggcgcgcctc gcgcacgagc gggcgcaggt
    15901 cgcccaccag cgcgcgcagg ttcacgagtg tggtgttcgc ctcgcgcagg gtcggaggca
    15961 gctggcgcag cgagctgtcg agcgaggcgg tccgctccgc gatggcgctg agggtcgcgc
    16021 cggtggccgg aacgagccgc tcgaggtcgg ggcgccgctc ggctacggcc gacaccacgc
    16081 tcgccgagtc cacgaggaag cgcgagaacg ccccctcgtc gcgcatgacc tcgcgggccg
    16141 tcgaggccgt ctgcgagagc gcggggttga gcgcgaccat cgcgtcctcg agctccttgc
    16201 cgcggccgtc ggtggccgcc ccgagcccgt ggacggtcgc ctggagatcg cccagcacct
    16261 cgggggacag ggagttcagc accgcgtcga ggtccacctc ggactccgcg tcggtggcgg
    16321 ctatggcgcc gccgtcctcg atcggctcct cgtcgttggg gcccggcttc agcgccacgt
    16381 agcggttggc gatgcccgac agcgaagtcg agcggacctc caccgtcgtg ccacggtgca
    16441 gcggctggag ctcctcgtcg gtgatgtcga gctcgaccag cgcctgcgcg tcgtcgccca
    16501 gctcgagctt cgacacggtc ccgaccggga ccccgcccac cttcacctgg ttgcccttga
    16561 cgagctggct cgcgttgtcg agcgtcaggg tgacgcggta cgagtcatcg gaggcgaaca
    16621 gcagccacgc gatcaccgcg atcgcgccaa gcagcgcagc gagcgtcagc agccgcgcga
    16681 gtacatgggt tcgcctgccg accggcacag agactcctcc ccggaaactc cagcggcgaa
    16741 gctatacgac tggcgccctg cgccgcgctg ctaagcggcc gcgagccgcc tcacgcgccg
    16801 gtccgcactg cgggccggcg gaaactcgcg caggagcttg ggcagcacgg gcagcgccgc
    16861 ggagagcacc aagcgcgagg cctcgaacag gcgcgcgcgg tgcggtcccc aggggagccc
    16921 gagctcgtcg cgcagcgacg tcgggaggtg accgaccgtc gacaggttca gggcctcgac
    16981 gatcgggcgc gccacgaacg gcagctccgg ccgcatcacg gcgtcgacca cgtcgagcag
    17041 cgcctcggtg ggcgcgatcc gctcgctggc gagcatcgcc tcgaagtagt cgttgaagtc
    17101 ggcgtaggtg gccggctggt gctcgcgcgg gacgccgaac ttctcaccga gcagcttctg
    17161 ctcctcgtag tagcggcgct gctcggcgat cgtgagcggc cgcacgtatc gctggtagac
    17221 gagcagcgag gtgtggacga gcgtggcgtg gacccacatg atcaggtcgt gtgtgcgcgc
    17281 gtcgtagggc gtgccctcgg cgtacgcgcc ggcggcctgg tcgctcttcc cgcgcacgcg
    17341 gccgtgcgtg tggcggatgc gcgccgaagc ctcctccgcg gtcgcggtcg agccgaactg
    17401 gatcttcgtg gtgaggtcga gcgtgcgata gagccggccc cacgggtcct cgcggtagtt
    17461 cgagtgctgc gaaacgcccg ctgcgaccag tggatgcgcg agctggagga tcagcgcggt
    17521 gccgccgccc gccagcagga caccctcgcg gttgacgcgc catgtgaccg agtcgggccc
    17581 gaacagccca gggtcgctca tcgcagccgc gatcgtaccc tgttgctcgg cgcacgatcc
    17641 gggtaccctg cgcgcatggg ctggggtttc gtctggatga tggtggtgct gaagctcccg
    17701 atcgtcgcgc tgctgtggct cgtctggtgg gccatccggc agaccgacgc ggaggccgag
    17761 ctgccgaccg acgacggcgg cagcgaccgc catccgcatc gcggcggccc gcgccgcccg
    17821 cgcccgcccc gccgcgggcc gcacgccgaa cccgccccgc gcgcgccaag ccgcatccgc
    17881 gtggcgggcc ggcagctcac ccgaccgctc aggtaggctg ccgcgccgtg agcctccccc
    17941 tggcggagtt caacgcgtac cacggcatcg gcatcgcgtt cgcggtctgg gccgtgatcc
    18001 tgtccttgct cggtatgagg agcgccgact tcccgcgcag cggcgggatc cagcgcgccg
    18061 tggtcgcgat cagcgcgctg ctcctggtca gcgcggtcgg cgcggccatc gccacgtcgg
    18121 agaagcacgg ggcgcacgag tcgagccacg gcgatcggga gaacccgacg catccgggcg
    18181 aaagccccga gaacgtgcca aacgagtgac ccgcgcccgg cgcgcggtgc gccgggggcc
    18241 tccgttcgtc agaatcaagc gttccgggta gtccagagcg cgaacagcga acgagaggat
    18301 cggcatggcg gaggagcaga cgcagtcaca ggacgggggt ggggtagcca ccgccgagca
    18361 gaccctcacg gtcaccgaca accgcaccgg cgagacgtac gaggtcgagg tctccgacgg
    18421 caccgtgaag gccatggact tccgccagat caaggtcgac gaggacgact tcggcctcat
    18481 gacctacgac ccggcgttca cgaacaccgc cgcctgccgg agcgagatca cctacatcga
    18541 cggcgaggcc ggggtgctcg agcatcgcgg ctacaagatc gaggagctct gcgagcgggc
    18601 gtcgtacctg gaggtcgcct acctgctcat ccacggtgag ctgccgaacc agtcgcagct
    18661 cgacgactgg acgcatgaga tcacgatcca cacgttcgtg cacgagaaca tcaagcggtt
    18721 catggagggc ttccgctacg acgcgcatcc gatggggatg ctgctcgcgt cggtcggcgc
    18781 gctctccacg ttctacccgg gtgccaagga catccaggac cactccgagc gccacatggc
    18841 cgcggtgcgg ctgatcgcga aggtgccgac gctcgccgcg ttcgcctacc gccactcgct
    18901 cgggctcccg tacaactatc ccgacaacga cctcgactat ccgagcaact tcctccagat
    18961 gatgttccgg atgaccgagc tcaagtacga gccggacccg cggctcgcga aggcgctcga
    19021 cgtgctctgg atcctgcacg ccgaccacga gcagaactgc tcgaccaacg cggtgcgcgc
    19081 ggtgggctcg tcgaaggtgg acccgtactc ggccgtggcc gccggcgtgg ccgcgctgta
    19141 cggcccgctg cacggcggcg ccaacgaggc ggtcctcaag atgctcgatc ggatcgggac
    19201 gaaggagaac atcccggact tcctcgaggg cgtgaaggaa ggcaaggagc gcctgatggg
    19261 cttcggccac cgcgtctaca agaactacga tccgcgcgcg cggatcatca agaagcacgt
    19321 ggacgaggtg ttcgaggtca ccggcgtgaa cccgaagctc gagctggccg tcgagctcga
    19381 gaagcgagcg ctcgacgacg agtacttcac cgaccgcaag ctctacccga acgtcgactt
    19441 ctactcgggc ctgatctacg aggcgctgac cctgccgccg gagatgttca cggtgatgtt
    19501 cgcgatcccg cgcacgtccg gctggatcgc gcagtggatc gagatgatcg acgacaagga
    19561 gcagaagatc gccaggccgc ggcagatcta caccggcgag cgcgaaagag aatttgtgcc
    19621 ggtggccgac cggggttagg ctggtcggtg ggcagctgcc cacaggggga cggcctatca
    19681 aggaggaaag catgaagaag ctggtggttg tggcggtgac ggcgatggcg ctggtcgcag
    19741 cggcgcccgc gagcgcggag cccggtaagg accagggctg cggcgacata ttcaggggcc
    19801 tgggggcgcc cgggttcggt caggcggtat ccgaggtcgc gggtgagacg ggcttcgacg
    19861 actacgtcca ggtcgtctgc cggcacttct agaccgggcc gaggtagcgc atgtagacct
    19921 gccggttctc cggcggcggc cgctgcggcg gcgccgccgg agggaccggg tcctcgtact
    19981 gggagctgcg cagcgagcgc tcctgacaga cgagaggaag gcggcgcacg acggcgccga
    20041 ccagaacgcg ccgtcgatcg cggcgcacca cgaagacgtc cttggcgccg ttggtgtccc
    20101 cccgcgtcag gtccgaggcc tcggattcga acgcgacgta gcgcgaccag cggcggtcct
    20161 gcgagatcac cgcgttcgtg gacgggccgt tcggcgtccc gccgtcgagc gcaccgggag
    20221 acgagctggg tgactggacg tcggcgcgcg acttggctgc gaccgcggct cgatcgccgg
    20281 cggtcgcgac gaagcagacg gctgcggcaa caggtagggg gtacgccctg ccaggcaccg
    20341 attgggaagg tggcggcgtt gcgctcccct acgcttcaca cgtggccgcg cagcgcgtct
    20401 ccctgccgag cgccatcgcc aagccggcgg cccggctcgg agcggccgcg cgcttgcgaa
    20461 tgcgcgacgc ggtccggtcg ctgcccccgc gctgggaggc cgtgcgcgac g
//
LOCUS       DASVPL010000236         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_340327, whole genome shotgun sequence.
ACCESSION   DASVPL010000236 DASVPL010000000
VERSION     DASVPL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340327"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VF517_12690"
     CDS             <1..1129
                     /locus_tag="VF517_12690"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX8053843.1"
                     /translation="AAPVRARLAALRALLAALRARLAAPVRALLAALRALLAARRAPM
                     RAAVLRGIPRAAGAFGHGAVALRGRFGGLSAAARRRVLAAFMLLALLATVYFAWFRDS
                     SFVAVEKVTVTGAVTDDAPRIRARLIAAARDMSTLHVDEEALRRALPAGAAVAELRVS
                     TDFPHGMRIEVVQRPAVAVLVGEGRRVAVAADGSLLKGVRAEHVPSIAVGAFPGRGQL
                     GRGRALQLVRVAAAAPAPLRARIERVRRLPDKGLVAYLRRGPQVILGEANGLDAKWAA
                     AAAVLADGASRGATYVDVSIPTRPVAGGVDVPQPEAEQPPAGSGGAPVDPGAGGTPGA
                     GGTPGAGTTPGASGTTPGAGTTPGATGTPGATGAPPSGTTP"
     gene            1468..2619
                     /gene="ftsZ"
                     /locus_tag="VF517_12695"
     CDS             1468..2619
                     /gene="ftsZ"
                     /locus_tag="VF517_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX8053844.1"
                     /translation="MELGAMDTGSYLAVIKVVGVGGGGTNAVNRMVDAGLKGVEFIAV
                     NTDAQSLQMCDADIKLQIGGQLTRGLGAGANPETGHGAASESRDEIKEALKGADMVFV
                     TAGEGGGTGTGAAPVIAEIAKHEIGALTVGVVTKPFLFEGSQRSRQAEEGIERLGEQV
                     DTLIVIPNEKLLGIVERRTSILDAFRMADDVLRQGVQGITDLITIPGLINLDFADVRT
                     IMHDAGSALMGIGAASGESRAAEAAKAAISSPLLEKSVAGATGILLNITGGSDLGLFE
                     VNEAAEIIQSAAASDSNIIFGAVIDEHGGDDLRVTVIATGFDRPSGGASAGSSGLFDV
                     QRAPRERDRPRRERPSDASAGRTPVFDDSERSSLEIGDDDIDVPSFLKD"
     gene            complement(2616..>3521)
                     /locus_tag="VF517_12700"
     CDS             complement(2616..>3521)
                     /locus_tag="VF517_12700"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8053845.1"
                     /translation="VRVQRQANGGKPAAMNRGIEAARSDVIVTVDGDTVFEPGTLREL
                     VRPFRDPDVGAVAGNTKVGNRGGLLGRWQHIEYVMGFNLDRRLYDLLRCMPTVPGAIG
                     AFRRAALVDIGGVSHATLAEDTDITIGIGRAGWRVAYAEDARAWTEAPASLAALWRQR
                     YRWSYGTMQAVWKHRAAVVRPGEWRIGRVAIPYLVLFQILLPLLAPLIDLFALYGIVF
                     LDPVPVVGYWLGFNLLNLLLGWYAFRLDRESPRPLWAMPLQQFVYRQLMYLVAIESVI
                     SALVGNRIRWQPMERTGAVEVPPAS"
BASE COUNT          500 a         1313 c         1267 g          441 t
ORIGIN      
        1 cgccgcgccg gtgcgcgcac ggctcgccgc gctccgcgca ctgctcgccg cgctccgcgc
       61 acggctcgcc gcgccggtgc gcgcactgct cgccgcgctc cgcgcactgc tcgccgcgag
      121 gcgcgcgccg atgagggcgg ccgtcctccg cgggatcccg cgcgccgccg gcgccttcgg
      181 ccacggcgcc gtcgcgctgc gcgggcgctt cggcggcctg tccgccgccg cgcgccggcg
      241 ggtcctcgcc gcgttcatgc tcctggcgct gctcgcgacc gtctacttcg cctggttccg
      301 cgactcgtcg ttcgtcgccg tcgagaaggt cacggtcacc ggcgccgtga ccgacgacgc
      361 gccgcgcatc cgcgcccgcc tcatcgcggc cgcgcgcgac atgagcaccc tccacgtgga
      421 cgaggaggcg ctgaggcgcg cgctcccggc cggcgccgcc gtcgccgagc tgcgcgtgag
      481 caccgacttc ccgcacggga tgcgcatcga ggtggtccag cggcccgcgg tggccgtgct
      541 cgtgggggag ggccgccgcg tggcggtggc ggccgacggc tcgcttttga agggcgttcg
      601 cgccgagcac gtgccgtcga tcgcggtcgg cgccttcccg gggcgcggcc agctcggtcg
      661 cggccgggcg ctccagctcg tccgggtggc cgccgcggcc ccggcgcccc tgcgtgcgcg
      721 gatcgagcgg gtccgccggc tgcccgacaa gggcctcgtc gcctacctgc gccggggccc
      781 gcaggtgatc ctcggcgagg cgaacggcct cgacgcgaag tgggccgccg ccgccgccgt
      841 gctcgccgac ggcgcgagcc gcggcgcgac ctacgtggac gtgagcatcc ccacccgccc
      901 cgtcgcgggc ggcgtggacg tgccgcagcc cgaggcggag cagcctccgg cggggagcgg
      961 cggcgccccg gtggacccgg gcgcgggcgg taccccgggc gcgggcggta ccccgggcgc
     1021 cggcacaacc ccgggcgctt cgggcacgac ccccggcgcc ggcaccaccc ccggcgctac
     1081 gggcaccccc ggcgccaccg gcgctccgcc ctccggcacc accccgtgac ccacccggtc
     1141 cgcggagtcc ggtgcgccgc gactacggca tcacttaggg ggagggaccg aactggtcga
     1201 tctaatacac gtatacggtg tacaagatcg accaggtccc cgggacggtc ccaaagtcta
     1261 aagctcgact tgaggcttgc gaccctcgac caccgctcca ggctttagcc ggggcgcacg
     1321 agatcttgcc gctgattgac acttcgcccg aaactcccct acagtgcgga atttgcggca
     1381 tcccatccgg gggtaaaact ctcaacaatc acttgagggt cggacggaat gaaggctccg
     1441 tctcgccgcc agtgggagga acctgaaatg gaactgggag cgatggacac aggtagctac
     1501 ctggcggtga tcaaggtcgt cggtgtcgga ggcggcggca cgaatgccgt caaccgcatg
     1561 gtcgacgccg gcctcaaggg ggtcgagttc atcgcggtca acaccgacgc gcagtcgctg
     1621 cagatgtgcg acgccgacat caagctccag atcggcggcc agctcacgcg cgggctcggc
     1681 gcgggcgcca acccggagac cggccatggc gcggccagcg agagccgcga cgagatcaag
     1741 gaggcgctga agggcgctga catggtcttc gtgaccgcgg gcgagggcgg cggcaccggc
     1801 accggcgcgg cccccgtgat cgccgagatc gccaagcacg agatcggcgc gctcaccgtc
     1861 ggcgtcgtga ccaagccgtt cctgttcgag ggcagccagc gctcccgcca ggccgaggag
     1921 ggcatcgagc gcctcggcga gcaggtggac acgctgatcg tgatcccgaa cgagaagctg
     1981 ctcgggatcg tcgagcgccg cacgagcatc ctcgacgcct tccggatggc cgacgacgtg
     2041 ctgcgccagg gcgtccaggg catcaccgac ctcatcacga tccccggcct gatcaacctc
     2101 gacttcgcgg acgtgcgcac gatcatgcac gacgccggct cggcgctgat gggcatcggt
     2161 gcggccagcg gcgagagccg ggcggccgag gccgcgaagg ccgcgatctc gagcccgctg
     2221 ctcgagaagt cggtcgcggg cgccacgggg atcctgctga acatcacggg cgggtccgac
     2281 ctcgggctgt tcgaggtcaa cgaggcggcc gagatcatcc agagcgcggc cgcgtcggac
     2341 tcgaacatca tcttcggcgc cgtgatcgac gagcacggcg gcgacgacct ccgcgtgacc
     2401 gtcatcgcca cgggcttcga ccgcccgagc gggggcgctt ccgccggcag ctccggcctg
     2461 ttcgacgtgc agcgcgcgcc gcgcgagcgc gaccgcccgc gccgcgagcg cccgtccgac
     2521 gcctcggccg gccgcacccc cgtcttcgac gacagcgagc gctcgtcgct cgagatcggc
     2581 gacgacgaca tcgacgtgcc gagcttcctg aaggactagc tcgccggcgg gacctcgacg
     2641 gcgccggtcc gctccatcgg ctgccagcgg atccggttgc ccaccagcgc gctgatcacc
     2701 gactcgatcg ccacgaggta catgagctgg cggtagacga actgctgtag cggcatcgcc
     2761 cacagcggcc gcggcgactc gcggtcgagc cggaacgcgt accagcccag cagcaggttg
     2821 agcaggttga acccgagcca gtagccgacg accggcacgg gatcgaggaa gacgatgccg
     2881 tagagggcga acaggtcgat cagcggcgcg agcagcggca gcaggatctg gaagagcacc
     2941 aggtacggga tcgccacgcg gccgatccgc cactcgcccg gacgcacgac cgcggcgcgg
     3001 tgcttccaga ccgcctgcat cgtcccgtac gaccagcggt agcgctggcg ccagagggcg
     3061 gcgagcgagg cgggcgcctc ggtccaggcc cgcgcgtcct ccgcgtaggc cacccgccag
     3121 ccggcgcggc cgatgccaat cgtgatgtcg gtgtcctcgg ccagcgtggc gtgcgagacg
     3181 ccgccgatgt cgacgagcgc ggcacgcctg aacgcgccga tggcgcccgg cacggtgggc
     3241 atgcagcgca gcaggtcgta caggcgccgg tcgaggttga agcccatcac atactcgatg
     3301 tgctgccagc ggccgagcag cccgccgcgg ttgcccacct tggtgttgcc ggccacggct
     3361 cccacgtccg ggtcgcggaa cgggcgaacc agctcgcgca gcgtgcccgg ctcgaacacc
     3421 gtgtcgccgt cgacggtgac gatcacgtcg ctgcgcgcgg cctcgatccc gcggttcatg
     3481 gcggcgggct tgccgccgtt cgcctggcgc tgcacccgca c
//
LOCUS       DASVPL010000237         8746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_145207, whole genome shotgun sequence.
ACCESSION   DASVPL010000237 DASVPL010000000
VERSION     DASVPL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145207"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VF517_12705"
     CDS             complement(<1..1116)
                     /locus_tag="VF517_12705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HEX8053846.1"
                     /translation="MRRPSAADLVDLVPAIRVLSRPVAGVAAIAALALAGCGDGSTPL
                     RDSVAFDGRSPRVPAAARVRVLVELRRPSLGDRMATEQLDPAAQRAHVASLASEAEAL
                     LSALRAKDVETRRPVLYGRAWSGFAVTLSTNDLPAVQALGLRSEPVRRFYGATSAVTP
                     AAGAVRVQRRARGRAAPTVALLDSGVSPRARPLRERVVAGYDAVDRDRDPAPRGSRER
                     HGTAMASVLAAELPAGERFASIRVAGRQRDTEGGPELEVGTTDQVLAGLERTVDPDGD
                     GDVEDRIPIALVGVSSPYSGFANSPEDESARAAAAIGTLVIAPAGNEGAATGSYGTIG
                     SPAAAPHALAVGALDGAAPGGAAAPGGGAGGGAAGGGS"
     gene            complement(1109..2101)
                     /gene="tsaD"
                     /locus_tag="VF517_12710"
     CDS             complement(1109..2101)
                     /gene="tsaD"
                     /locus_tag="VF517_12710"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX8053847.1"
                     /translation="MILAIETSCDDTCAAVVGSDGEVRSNVVASQGLLHERYGGVVPE
                     IASRRHLELIDAVLDDALERAGATLDDASVVAVTRGPGLIGALLVGVSAAKALAAARG
                     LPLVPVDHLAGHVLASTLGERPIEPPFVCLVASGGHTFLARVDEPPGYTILGQTLDDA
                     AGEAFDKGARLLGLGYPGGPAVDRLARSGDPEAFDFPRALPGAGLDFSFSGLKTALLY
                     RVRDLGEEVAAARQADLAASYQRAIVESLVARLRQAVEREGVGRVAIGGGVAANSELR
                     RRVEELAGELGAELWVPPMELCTDNAAMIAAVARYLDPIPYPGYLGLDATARVA"
     gene            complement(2098..2583)
                     /gene="rimI"
                     /locus_tag="VF517_12715"
     CDS             complement(2098..2583)
                     /gene="rimI"
                     /locus_tag="VF517_12715"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX8053848.1"
                     /translation="MKKQLEIRRLAYADLPQAIAIERRSFPTPWSLAMFVLELSKPSG
                     ICLAALQDGRIVGYLICSRYDTVWHVMNVAVDPNLRRRGIGTRLLERLFERADRPGAQ
                     YTLEVRLSNEGGIALYESFGFYSAGLRRGYYHDNKEDALIMWRTVPGQEEPGGVAAAH
                     A"
     gene            complement(2580..3251)
                     /gene="tsaB"
                     /locus_tag="VF517_12720"
     CDS             complement(2580..3251)
                     /gene="tsaB"
                     /locus_tag="VF517_12720"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX8053849.1"
                     /translation="MTVLGFDTSTAATSACLLRADGEAFESVPTPAELFGRPGHAREL
                     LPRAAAVMERAGVGFGDLAAVAVGVGPGTFTGLRIGVATARGLAHAHGMELRPVSSLA
                     ALAAGIDAPLRLALIDARRGELFGALHDEAGERWEEFVAAPEALAGRVHELDDRPLAA
                     GDGAVRFRELLEAAGVRVAPDEAEAHAVRALHVCRLAATAQPVAPEAVLPNYLRAPDA
                     IPRQA"
     gene            complement(3248..3673)
                     /gene="tsaE"
                     /locus_tag="VF517_12725"
     CDS             complement(3248..3673)
                     /gene="tsaE"
                     /locus_tag="VF517_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX8053850.1"
                     /translation="MRLETANPDATAEAGASLATRLRGGDTVLVGGELGSGKTTFVRG
                     ACRALGVDGPVTSPTFTIGQVYGGRTPDGAPIEIAHLDLYRLQTLAGEDPALLEDYLT
                     PDRIAFVEWPGVALDAIPRIAARVTIQHLGGDRRVIEVR"
     gene            3738..4067
                     /locus_tag="VF517_12730"
     CDS             3738..4067
                     /locus_tag="VF517_12730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8053851.1"
                     /translation="MNTLSLETREEGEQVRIAVAGELDLSSALTFDEEVRRAEERKPA
                     VLVIDLSGLKFLDSTGLRLIMSAQARARKHGCRLKIVEGGEAVKRIFRLAGVHRRLDI
                     VSEAPAA"
     gene            complement(4087..4899)
                     /locus_tag="VF517_12735"
     CDS             complement(4087..4899)
                     /locus_tag="VF517_12735"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX8053852.1"
                     /translation="MSDRTPEERREELIRLYHEVRDCRRCPLSATRTNAVFGMGNADA
                     DLMFVGEAPGAEEDRQGKPFVGRAGKLLDQLLADVGMERSRDVFIANVLKSRPPGNRD
                     PQPDEIEACRPYLETQIALIEPLVICTLGNFATKLLTGSPVGITRCCGVPQERELGGR
                     PVVIYPLFHPAAALRTPNVLERLREDFSRVPALLERERARSAAAAPESASAEPDRAGS
                     AEPDRAGSAGQDRAGSAEPERPSALAAMAASMGTTAASEPDPPPQLGLFGSA"
     gene            5020..5766
                     /locus_tag="VF517_12740"
     CDS             5020..5766
                     /locus_tag="VF517_12740"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX8053853.1"
                     /translation="MSAANGTARTPARIGIVGRGRVGEALAAGLRRAGREVEGPLGRG
                     EAPDSDVVLLCVPDSEIAAAAEHVRASARTVGHTSGATSLAVLGEGEAFGLHPLQTVA
                     GEARLAGAGCAIAGSSPAALATARELATALGMEPFEIDDSQRAAYHAAASIASNYLLA
                     LEAAAETVAAGAGIPRADARRMLGPLVRQTVENWQRLGPERALTGPVARGDEETVARQ
                     AEAVENVAPELARLFAELTEATRGLTGVAA"
     gene            5763..6629
                     /gene="panC"
                     /locus_tag="VF517_12745"
     CDS             5763..6629
                     /gene="panC"
                     /locus_tag="VF517_12745"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX8053854.1"
                     /translation="MRTVRTIAELRAALAARRAGGDIGLVPTMGAFHEGHLSLMRQAR
                     ADCATVVVSLFVNPAQFSPNEDLSAYPRDEARDAGLASDEGVDLLFAPPHEEVYPDGF
                     DTTVEVHGLTEILCGDPAHRGAAHFRGVTTVVTKLFNMVHPDVAYFGQKDAQQAAVMR
                     KLVRDLDMPVRIEVLPTVREPDGLALSSRNAYLSPAEREKALALSRALAAAGEEVAAG
                     RRDAASVLAAARRELDSRGVEPEYLELRSADDLSPAERVNGRTLLAVAAHVGRARLID
                     NAMLGDPNGGHP"
     gene            6638..7006
                     /gene="panD"
                     /locus_tag="VF517_12750"
     CDS             6638..7006
                     /gene="panD"
                     /locus_tag="VF517_12750"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX8053855.1"
                     /translation="MLKSKIHRGRVTGSDLHYVGSITVDADLLDAADILEHEQVHVLD
                     IDNGARFETYTIAGERGSGEMCINGAAARLVHTGDRIIVVSYAEYSAEDLESYVPRVV
                     HVDEHNGIVTVDDAVAELLS"
     gene            7003..7905
                     /gene="panB"
                     /locus_tag="VF517_12755"
     CDS             7003..7905
                     /gene="panB"
                     /locus_tag="VF517_12755"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX8053856.1"
                     /translation="MSSGPRISPAPPRKRATVTLSTLAEKKALGEPIVMVTAYDYPSA
                     QVAEAAGVDVVLVGDSGAMTVLGYPSTVPVSVDEMLMLAGAARRGLSTPLLVGDLPFG
                     SYEVSNEQAVATAQRFVKEAGCDAVKLERGGVCAERARAIVDAGIPVMGHVGLTPQTA
                     IALGGYRSQGRTAERALEVANDAFALEEAGCFAIVFEAVPSALTEALVPRLEVPVIGI
                     GAGPDTDGQVLVFHDLLGIYDGHAARFVKRYGNVRQAMVDGVEAFTADVRERRYPEPE
                     HGYTMAPDEAERLRTLLTEHQPAS"
     gene            7955..8371
                     /locus_tag="VF517_12760"
     CDS             7955..8371
                     /locus_tag="VF517_12760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX8053857.1"
                     /translation="MATHGGELRFETEGDGHVVDLTDGVAAVVRQSGIENGTASVFVP
                     GSTAAVTTMEYEPGGVQDLRVLLDRLIPREGDYEHNRLNHDSNSHAHMRAAIVGPSET
                     FPVIGAELRLGTWQQVVLIDFDDRPRQRTVVVSVTG"
     gene            8371..>8746
                     /locus_tag="VF517_12765"
     CDS             8371..>8746
                     /locus_tag="VF517_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053858.1"
                     /translation="MRLRPALAAGLALALTLGALAAPARAAEVTRSPDATAVSINGLF
                     HYSGALLPNASQHLGLIARSGIRVARAGAFWPWVEPRAPFLGFHSYTWDSTDDAIGEL
                     AANGLRFLPVLGYSTLWSTTVPN"
BASE COUNT         1152 a         3322 c         3169 g         1103 t
ORIGIN      
        1 gctgccgccg cccgccgcgc cgccgcccgc gccacctccg ggagccgcgg cgcctccggg
       61 agccgcgccg tccagcgcgc ccaccgcgag cgcgtgcggc gccgcggccg gggagccgat
      121 cgtcccgtac gagcccgtgg ccgcgccctc gttgcccgcc ggcgcgatca ccagcgtgcc
      181 gatagcggcc gccgcgcgcg ctgactcgtc ctccggcgag ttcgcgaagc ccgagtacgg
      241 ggagctgacg ccgaccagcg cgatcgggat ccggtcctcg acgtcgccgt cgccgtcggg
      301 gtcgaccgtc cgctcgagcc ccgcgagcac ctggtcggtc gtaccgacct ccagctccgg
      361 gccgccctcc gtgtcgcgct ggcggccggc gaccctgatc gaggcgaagc gctcgcccgc
      421 cggcagctcc gcggcgagca cggaggccat ggcggtcccg tgtcgctccc gcgacccgcg
      481 cggcgcggga tcgcggtcgc gatcgacggc gtcgtagccc gccaccaccc gctcccgcag
      541 gggccgcgct cgcggggaca cccccgagtc gaggagcgcg acggtgggag ccgcgcggcc
      601 gcgcgcacgt cgctggaccc gcaccgcgcc cgccgcgggg gtcacggcgg atgtggcccc
      661 gtagaacctg cgcaccggct ccgagcgcag gccgagcgcc tggacggcgg ggaggtcgtt
      721 ggtcgagagc gtcaccgcaa agccgctcca cgcgcggccg tagaggacgg gccgcctcgt
      781 ctcgacgtcc ttcgcgcgca gcgcgctcaa cagggcctcg gcctcgctcg cgaggctcgc
      841 cacgtgcgcg cgctgggcgg cgggatcgag ctgctcggtc gccatgcggt cgccgagcga
      901 cgggcggcgc agctccacga gcacccgcac ccgcgcggcg gcgggcacgc gcgggctgcg
      961 gccgtcgaag gcgaccgagt cgcgcagcgg cgtgctccca tccccgcatc cggcgagcgc
     1021 gagggcggca atcgccgcga cgcccgcaac tggtcgactt agtacccgta tagcgggcac
     1081 aaggtcgacc aggtcggccg cggagggtct acgcaacccg cgcggtggcg tcgaggccga
     1141 ggtagccggg gtacgggatc gggtcgaggt accgcgccac cgccgcgatc atcgccgcgt
     1201 tgtccgtgca cagctccatc ggaggcaccc acagctcagc ccccagctcg cccgccagct
     1261 cctccacccg ccggcgcagc tcggagttcg ccgccacgcc acccccgatc gccactcgcc
     1321 ccactccctc gcgctccacc gcctgacgca gccgcgcgac gagcgactcc acgatcgcgc
     1381 gctggtacga ggcggccagg tcggcctgcc gggccgcggc gacctcctca cccaggtcgc
     1441 gcacgcggta gaggagcgcc gtcttgaggc cgctgaacga gaagtcgagc ccggcgcccg
     1501 gcagcgcacg cgggaagtcg aacgcctccg gatcgcccga gcgggctagc cggtccacgg
     1561 cggggccgcc cgggtagccg aggccgagga gccgcgcgcc cttgtcgaac gcctcgccgg
     1621 cggcgtcgtc gagcgtctgg ccgaggatcg tgtagccggg cggctcgtca acgcgggcga
     1681 ggaaggtgtg cccgccgcta gcgaccaggc agacgaacgg cggctcgatc ggccgctcgc
     1741 cgagcgtgct cgccagcacg tgcccggcca ggtggtccac cggcacgagc gggagcccgc
     1801 gcgcggccgc aagcgccttt gcggccgaga ctcccaccag cagcgcgccg atcagccccg
     1861 gcccgcgcgt gaccgccacg acggacgcgt cgtcgagcgt cgccccggcg cgctcgagcg
     1921 cgtcgtcgag cacggcgtcg atcagctcga ggtggcgccg cgaggcgatc tccggcacca
     1981 ccccgccgta gcgctcgtgg agcaggccct gcgaggccac gacgttcgat cgcacctcgc
     2041 catcgctgcc gacgaccgcg gcgcaggtgt cgtcgcagct cgtctcgatc gcgaggatca
     2101 cgcgtgtgcg gcggcgacgc cgccgggctc ttcctgtccc gggaccgtcc gccacatgat
     2161 cagggcgtct tccttgttgt cgtggtagta gccgcggcgc agtcccgcgg agtagaagcc
     2221 gaagctctcg tacagcgcga tgccgccctc gttcgagagc cggacctcga gcgtgtactg
     2281 ggcgccgggg cggtcggcgc gctcgaacag ccgctcgagc agcctggtcc cgatgccgcg
     2341 ccggcgcagg ttcgggtcga cggccacgtt catcacgtgc cagaccgtgt cgtagcgcga
     2401 gcagatcagg tagccgacga tgcgcccgtc ctggagcgcg gcgaggcaga ttccggacgg
     2461 cttcgatagc tcgagcacga acatcgcgag cgaccacggc gtcgggaacg agcggcgctc
     2521 gatggcgatc gcctgcggca ggtccgcgta ggccaggcgg cggatctcga gttgcttctt
     2581 catgcttgtc gcgggattgc gtccggggct ctgaggtagt tcgggaggac ggcctccggg
     2641 gcgaccggct gggcggtggc tgcgagccgg cacacgtgca gcgcgcggac cgcgtgcgcc
     2701 tccgcctcgt cgggcgcgac ccggacgcct gcggcctcca ggagctcgcg aaatcgtacc
     2761 gccccgtccc ctgctgcaag cggcctgtcg tccagctcgt gcacgcgccc ggcgagcgcc
     2821 tcgggcgccg ccacgaactc ctcccagcgc tcgccggcct cgtcgtggag cgccccgaac
     2881 agctcgccgc gccgggcgtc gatcagcgcc aggcgcagcg gcgcgtcgat cccggctgcg
     2941 agcgcggcca gcgacgacac cggccggagc tccatgccgt gcgcgtgcgc gaggccgcgc
     3001 gcggtcgcga cgccgatccg caggcccgtg aaggtgcccg gcccgacacc gaccgcgacc
     3061 gcggcgaggt cgccgaaccc cacccccgcc cgctccatga cggccgccgc ccgcggcagc
     3121 agctcgcgcg cgtgcccggg ccggccgaac agctccgccg gggtggggac cgactcgaat
     3181 gcctcaccgt ccgcgcgcag caggcaggcc gaggtcgccg ccgtcgaggt gtcgaagccg
     3241 aggaccgtca tcgcacctct atgacacgcc gatcgccccc gagatgctgg atcgtcacgc
     3301 gcgcggcgat ccgcgggatg gcatcgaggg cgacgcccgg ccactcgacg aaggcgatcc
     3361 ggtccggggt caggtagtcc tcgagcagcg ccgggtcctc gcccgcgagc gtctggagcc
     3421 ggtagaggtc gaggtgggcg atctcgatcg gcgcgccgtc cggcgtgcgc ccgccgtaga
     3481 cctggccgat cgtgaacgtc gggctcgtga ccggcccatc gacgccgagc gcccggcacg
     3541 cgccgcgcac gaaggtggtc ttccctgagc ccagctcacc ccccaccagg acggtgtcgc
     3601 cacccctcag acgggtggcc agcgacgcgc cagcttcggc ggtcgcgtcc ggattggcgg
     3661 tttcgagtcg cattctcggg gagatttcat agcgcgaagg gcttcggtcc gcacacaggg
     3721 ggtatggtgg ccgccagatg aacacgctct cgctcgagac gcgtgaagag ggggagcagg
     3781 tacgtatcgc agttgcaggt gagctcgacc tctccagcgc gcttactttc gatgaagagg
     3841 tccgccgcgc ggaggagcgc aagcccgcgg tcctcgtgat cgacctgagc gggctgaagt
     3901 tcctcgactc cacgggcctg cggctgatca tgagcgccca ggcgcgggcg cgcaagcacg
     3961 gctgccgcct gaagatcgtg gagggcggcg aggccgtgaa gcgcatcttc cggctcgccg
     4021 gcgtgcaccg gcgcctcgac atcgtgtccg aggccccggc cgcctgagcg tccagcgggt
     4081 ccgccctcag gcggagccga acagcccgag ctgcggcggg gggtccggct cgctcgcggc
     4141 ggtggtgccc atcgacgcgg ccatggccgc gagggcggag gggcgctcgg gctccgcgga
     4201 gccggcgcgg tcctgcccgg cggagccggc gcgatccggc tcggcggagc cggcgcggtc
     4261 cggttcggcg gatgcgctct ccggcgccgc ggcggcactc cgcgcccgct cgcgctcgag
     4321 cagcgccggc acgcggctga agtcctcgcg caggcgctcg agcacgttcg gggtccgcag
     4381 cgccgcggcg gggtggaaga gcgggtagat cacgaccggc cggccgccca gctcgcgctc
     4441 ctgcggaacg ccgcagcagc gggtgattcc aacgggcgag ccggtgagca gcttggtggc
     4501 gaagttgccg agcgtgcaga tcacgagcgg ctcgatcagt gcgatctggg tctcgaggta
     4561 cgggcggcag gcctcgatct cgtccggctg ggggtcgcgg ttgccgggcg ggcggctctt
     4621 gaggacgttc gcgatgaaga cgtcgcgcga gcgctccatc ccgacgtcgg ccaggagctg
     4681 gtccaggagc ttcccggctc gccccacgaa gggcttgccc tggcggtcct cctccgcccc
     4741 cggcgcctcg cccacgaaca tcaggtcggc gtccgcgttg cccatgccga acacggcgtt
     4801 tgtgcgcgtg gcgctgagcg gacagcgacg gcagtcgcgc acctcgtggt agagccggat
     4861 cagctcctcg cgccgctcct ctggcgtgcg atcgctcacg tccggaaggt taggccgggc
     4921 ctcggctgtt tctcgccccg tttggggggt atcctcgggg ccaaggccgt tacccccgtg
     4981 gagcggacca accgactcac cagcatcgat cgccgtccga tgagcgccgc caacggcacc
     5041 gcacgtacgc ccgcccgcat cgggatcgtg ggtcgtggcc gcgtgggcga agcgctggcc
     5101 gccggtctgc gccgggcggg acgtgaggtc gagggcccgc tcgggcgcgg cgaggcgccc
     5161 gactcggacg tcgtgctgct ctgcgtcccg gactcggaga tcgccgcggc ggccgagcac
     5221 gtccgcgcct ccgcgcgaac cgtgggccac acgagcggcg cgacctcgct cgcggtgctg
     5281 ggcgagggcg aggcgttcgg cctccacccg ctccagacgg tggcgggcga agccaggctc
     5341 gccggcgcgg gctgcgccat cgcgggctcc tcccccgccg ccctcgccac cgcgcgcgag
     5401 ctggccaccg ccctcggcat ggagcccttc gagatcgacg actcgcagcg ggccgcctac
     5461 cacgcggccg cgtcgatcgc ctcgaactac ctgctcgcgc tggaggccgc ggcggagacc
     5521 gtcgccgccg gcgccgggat cccgcgtgcg gacgcgcggc ggatgctcgg cccgctcgtg
     5581 cgccagaccg tggagaactg gcagcggctc ggcccggagc gcgccctgac cggaccggtg
     5641 gcgcgcggcg acgaggagac ggtggcccgc caggccgagg ccgtcgagaa cgtcgcgccc
     5701 gagctggcgc ggctgttcgc ggagctcacc gaggccacgc gtggcttgac gggggtggcg
     5761 gcgtgaggac cgtgcgcacg atcgcggagc tgcgcgccgc cctcgccgcc cgccgcgccg
     5821 gcggcgacat cggcctcgtt ccgaccatgg gcgccttcca cgagggccac ctcagcctca
     5881 tgcgccaggc ccgcgccgac tgcgccacgg tcgtcgtctc gctattcgtg aaccccgcgc
     5941 agttctcccc caacgaggac ctgagcgcct acccgcgcga cgaggcgcgc gacgccggcc
     6001 tcgcctccga cgagggcgtc gacctcctgt tcgccccgcc gcacgaggag gtctaccccg
     6061 acgggttcga cacgacggtc gaggtccacg gcctcaccga gatcctctgc ggcgatccgg
     6121 cccaccgcgg cgcggcccac ttccgcggcg ttaccaccgt ggtcacgaag ctcttcaaca
     6181 tggtccaccc cgacgtggcc tacttcggtc agaaggacgc tcagcaggcg gccgtgatgc
     6241 gcaagctggt gcgcgacctc gacatgccgg tgcggatcga ggtgctgccc acggtccgcg
     6301 agccggacgg cctcgcgctc agctcccgca acgcctacct gtcacccgcc gagcgcgaaa
     6361 aggcactcgc gctcagccgc gcgctggccg ccgcgggcga ggaggtcgcc gccggccgcc
     6421 gcgacgccgc ctccgtgctg gccgccgccc gccgcgagct cgactcgcgc ggcgtcgagc
     6481 ccgagtacct cgaacttcgt tccgcggacg acctctcgcc ggccgagcga gtgaacggac
     6541 gcacccttct cgcagtcgcc gcgcacgtgg ggagggcgcg gctcatcgac aacgcaatgc
     6601 tcggagatcc caacgggggg cacccatgag acgcaccatg ctgaagtcca agatccaccg
     6661 cggccgggtg accggcagcg acctccacta cgtcggctcg atcacggtcg acgccgacct
     6721 cctcgacgcc gccgacatcc tcgagcacga gcaggtgcac gtgctcgaca tcgacaacgg
     6781 cgcccgcttc gagacctaca cgatcgccgg cgagcgcggc tcgggcgaga tgtgcatcaa
     6841 cggggcggcc gcgcggctgg tccacacggg cgacaggatc atcgtcgtct cgtacgccga
     6901 gtacagcgcc gaggacctcg agtcctacgt gccgagggtc gtgcacgtcg acgagcacaa
     6961 cggcatcgtc acggtcgacg acgcggtcgc ggagctgctg tcatgagctc cggcccgcgc
     7021 atctcccccg cccccccgcg caagcgcgcc acggtcacgc tctcgacgct ggccgagaag
     7081 aaggccctcg gcgagccgat cgtcatggtc accgcgtacg actacccgag cgcgcaggtg
     7141 gccgaggccg ccggcgtgga cgtcgtgctc gtcggcgact ccggcgctat gaccgtgctc
     7201 ggctacccca gcaccgtgcc ggtgtcggtc gacgagatgc tgatgctcgc cggcgccgcc
     7261 cggcgcgggc tcagcacccc gctgctcgtg ggcgacctgc cgttcgggtc ctacgaggtc
     7321 tcgaacgagc aggcggtcgc caccgcccag cggttcgtga aggaggccgg ctgcgacgcc
     7381 gtcaagctcg agcgcggcgg tgtctgcgcc gagcgcgccc gcgcgatcgt ggacgccggc
     7441 atcccggtca tgggccacgt cggcctcacg ccgcagaccg cgatcgcgct cggcggctac
     7501 cgctcgcagg gccgcaccgc ggagcgcgcg ctggaggtcg ccaacgacgc cttcgcgctc
     7561 gaggaggcgg gctgcttcgc gatcgtgttc gaggcggtgc cgtcggcgct caccgaggcg
     7621 ctcgtgccgc ggctcgaggt gccggtgatc ggcatcggcg ccggccccga caccgacggc
     7681 caggtgctcg tgttccacga cctgctcggc atctacgacg ggcacgccgc ccgcttcgtg
     7741 aagcgctacg gcaacgtgcg ccaggcgatg gtcgacgggg tcgaggcgtt caccgccgac
     7801 gtgcgcgagc gccgctaccc ggagcccgag cacggctaca cgatggcccc ggacgaggcc
     7861 gagcggctgc ggacgctgct caccgagcac cagccggcga gctgacggac gccaagcttc
     7921 gctggccgac ggcccgatcg gcgacactgc cgccgtggcc acgcacggcg gcgagctccg
     7981 cttcgagacc gagggcgacg ggcacgtcgt cgacctgacc gacggcgttg ccgccgtggt
     8041 gcggcaatcc gggatcgaga acggcacggc gtcggtgttc gtgcccggct cgaccgctgc
     8101 cgtcacgacg atggagtacg agcccggcgg cgtgcaggac ctgcgcgtgc tgctcgaccg
     8161 cctgatcccg cgcgagggcg actacgagca caaccggctc aaccacgact cgaactcgca
     8221 cgcgcacatg cgcgccgcga tcgtcggccc gtcggagacc ttcccggtaa tcggcgcgga
     8281 gctgcggctc ggcacctggc agcaggtggt gctgatcgac ttcgacgaca ggccgcgcca
     8341 acggaccgtg gtagtgagcg tgaccggctg atgcggcttc gccccgcgct ggccgcgggc
     8401 ctggccctgg cgctcacgct tggcgcgctc gccgctccgg cgcgggccgc cgaggtcacg
     8461 cgctcgccgg acgccaccgc cgtcagcatc aacggcctct tccactacag cggggcgctg
     8521 ctcccgaacg cgtcccagca cctcggcctg atcgcgcgca gcggcatccg cgtcgcgcgc
     8581 gcgggcgcct tctggccgtg ggtggagccg cgcgcgccgt tcctcggctt ccactcctac
     8641 acctgggact cgaccgacga cgccatcggg gagctggcgg cgaacggcct gcgcttcctg
     8701 ccggtgctcg gctactcgac cctctggtcg acgaccgtcc cgaacg
//
LOCUS       DASVPL010000238         1786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_223399, whole genome shotgun sequence.
ACCESSION   DASVPL010000238 DASVPL010000000
VERSION     DASVPL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223399"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1035
                     /locus_tag="VF517_12770"
     CDS             124..1035
                     /locus_tag="VF517_12770"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8053859.1"
                     /translation="MELPEDQRALLPSSGDVEFYEEHGWWVSPPIFSDEQMDEALRSV
                     ERHYAGERDSAPPTQLASYLDWTPEKGEDRLRINNYIALQQRGLGALALSPLLGATAA
                     RLARTEEVRLFNSSLVYKPPQVEGDAAKVGWHVDRAYWQTSTSENMLTAWVAFHDCDE
                     EMGTITMLNGSHRWPSDPVLDELRTGRTFVTDDVDALEQRLESLGLPIEKVPINVRRG
                     QVSFHHCMTFHGSGTNRSDRPRISMTVHMQDEQNRYREAYDAAGELFVYRNDTVCRKL
                     PSGEPDYRDPDICPVIWRDQDAVPASV"
     gene            1035..>1786
                     /locus_tag="VF517_12775"
     CDS             1035..>1786
                     /locus_tag="VF517_12775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8053860.1"
                     /translation="MPGDGAAVEPAAEPFALSAPYDPPQLDASLLAMSGLEQLRASIR
                     GETRPAPISELLGMRPIEADEGSAAFAMPASGWLAGEGGRPQAGVLAPLADAAHAAAV
                     VSTLPPGHALTTLCLTLSYVEALTGEAEEVVCRARCDRGGVGELPSHSSAEVTDDRGT
                     VLARSTARCAVFPLPGANAAGAAAAGAAASGGTAAGGAAAGGTAAGGTAAGGAAAGGA
                     AGDRTGAPAPRGTAAVPAPGLDASGDPPIRELT"
BASE COUNT          263 a          656 c          658 g          209 t
ORIGIN      
        1 gacgcgctcg agatcacgac cgtgggggac gtcgaggaga tcatcggacg ggtgctctcc
       61 ggcggcccgt cctgacacgc aaaagttcca ttagcgctgc aggtggtgat aaacagtgcc
      121 gccgtggagc tgcccgagga ccaacgcgca ctgctgccgt cgtcgggaga cgtggagttc
      181 tacgaggagc acggctggtg ggtgtccccg ccgatcttct cggacgagca gatggacgag
      241 gcgctgcgca gcgtggagcg ccactacgcc ggcgagcgcg actccgcccc gccgacgcag
      301 cttgccagct acctcgactg gacccccgag aagggcgagg accggctgcg gatcaacaac
      361 tacatcgcgc ttcagcagcg gggcctcggg gcactggccc tctcgccgct gctcggcgcg
      421 accgcggcac ggctcgcccg caccgaggag gtccggctct tcaacagctc gctggtctac
      481 aagccgccgc aggtggaggg cgacgcggcg aaggtcggct ggcacgtgga ccgcgcctac
      541 tggcagacca gcacatccga gaacatgctc accgcctggg tcgccttcca cgactgcgac
      601 gaggagatgg gcacgatcac gatgctgaac ggcagccacc gctggccgag cgacccggtg
      661 ctggacgagc tgcggacggg caggaccttc gtgaccgacg acgtggacgc gctcgagcag
      721 cggctcgagt cgctcggcct gccgatcgag aaggttccga tcaacgtgcg ccgggggcag
      781 gtcagcttcc accactgcat gacgttccac gggagcggca ccaaccgctc cgaccgcccg
      841 cggatatcca tgaccgtcca catgcaggac gagcagaacc ggtaccgcga ggcgtacgat
      901 gccgcgggcg agttgttcgt ctaccgcaac gacacggtgt gccggaagct gccgagcggc
      961 gagcccgact accgcgaccc cgacatctgc ccggtgatct ggcgcgacca ggacgcggtg
     1021 cccgccagcg tctgatgccc ggtgacgggg cagccgtaga gcccgccgcc gagccgttcg
     1081 cgctctcggc accgtacgac ccgccccagc tcgacgcgtc gctgctggcg atgagcgggc
     1141 tcgagcagct gcgcgcgagc attcgcggcg agacgcgccc cgcgccgatc agcgagctcc
     1201 tggggatgcg cccgatcgag gccgacgagg gctcggccgc gttcgcgatg ccggcgtccg
     1261 gctggctggc gggcgagggc gggcggcccc aggccggggt gctcgcccca ctcgccgacg
     1321 ccgcccacgc cgcggcggtc gtctcgaccc tgccgcccgg ccacgcgctc accacgctct
     1381 gcctgaccct gagctatgtc gaggccctca cgggcgaggc cgaggaggtg gtctgccgcg
     1441 cgcgctgcga ccgcggcggg gtgggtgagc tgccctccca ctcgagcgcg gaggtgaccg
     1501 acgaccgcgg caccgtgctg gcgcgctcga cggcccgctg cgccgtcttc ccgctgccgg
     1561 gagcgaacgc ggccggagcc gccgcggccg gagccgccgc gagcggaggc accgctgccg
     1621 gaggcgccgc ggccggaggc accgcggccg gaggcaccgc ggccggaggc gccgcggccg
     1681 gaggcgccgc gggcgaccgt accggcgcgc cggcgccgcg cgggaccgcc gcggtgcccg
     1741 cgcccgggct cgacgcgagc ggcgatccgc ccatccgcga gctgac
//
LOCUS       DASVPL010000239         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_107915, whole genome shotgun sequence.
ACCESSION   DASVPL010000239 DASVPL010000000
VERSION     DASVPL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107915"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VF517_12780"
     CDS             <1..692
                     /locus_tag="VF517_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8053861.1"
                     /translation="VAGDLYVIAPDDMHDGRDLGTTRGWMLGFMPDALLPDSPSTPGY
                     TPLPGDPRWLALRRTGARRSARYVVPPRDRGRWSQRFAEIRHELRARRLGYQHAVRAH
                     LGLLLIATTRAAMFDGPPVDPTPDPVQEMIEVIDARYAEPLSVGDVAQAVASSPSHLG
                     RMAKRVTGVSIRQWIEERRMQEARRLLLETDQSIDLIARTVGFHDVQYFRRRFRRAHG
                     MPPQTWRERNR"
     gene            826..1485
                     /locus_tag="VF517_12785"
     CDS             826..1485
                     /locus_tag="VF517_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8053862.1"
                     /translation="MRITSTTNSNFDAEAGLPADQQRRLRVLVVDDHDVVQWGFRMLL
                     GSESWVQRCIAAREPEEALRLATRYEPHVALIDVRLGEESGAELCTRLIEVSPMTRVL
                     LMSGFGQVSASVARGAGARGFVPKSWDAHDIAGAARMVGLGMTVFVPVQEQPVRGLTD
                     REQEVLPLIASGATNREIAASLFLSTNTVKDHTRTLYRKLRAKNRADAIVRAQRMGLL
                     D"
     gene            1606..2616
                     /locus_tag="VF517_12790"
     CDS             1606..2616
                     /locus_tag="VF517_12790"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX8053863.1"
                     /translation="MTEAGLGTWAGPVRPARAVRGAAIASLGAALPRTTVPNDQIAPR
                     LGVDDRWILKRIGVRERRMIAPDERLSDLVTAAARQALGRAEVDPEDLDLVLVGTFTQ
                     DEVLPNAAPLVAAALGATRAGGVDVGAACMGFLSGVALAAGIIEAGRADSVLVAGAEV
                     LTRYLDADDRLTAALIGDGAGAVVMTAVGAPSRIGPCVLRCDDRSRDVVYMTRNDMKL
                     RMEAQQTFVHAVDYLCDVTHEAVAAAGLTLDEIDLFVYHQANARITTSVGDRLELPPE
                     RVVDCIETLGNTSAASIPLALDVAQRECRLHPGMRVLVAAIGAGFTWGAAVIEWSAGG
                     VR"
     gene            2628..3842
                     /locus_tag="VF517_12795"
     CDS             2628..3842
                     /locus_tag="VF517_12795"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX8053864.1"
                     /translation="MITGTGAVTPLGLGSRALIERWVEGTSGIEDGLGRCSEFDPSEV
                     LTVKEVRRSDRFTQFAIAACAEALESAGWADGLPYASDRVGCVVGTGIGGLATIEEQH
                     SRLLDAGERSVSALGVPMMMSNAAAGALAMRHGMTGPSWGVVSACAAGAHAIGTAVRL
                     VQTGDADAVVTGGAEAALTPMARAGFARMGATSPTGVSRPFDARRDGFVLGEGAGILV
                     LEEREAALARGAQPIGEVLGYCATADAYHLTAPQPTGECAARAIAGALAEAGLDAARV
                     DYVNAHGTSTPLNDRTETQALKSALGDRAATVPVSSLKSAIGHLLGASGAVEAIATAL
                     ALRERVAPPTLNWSEPEEGLDLDYVPGVARPLRERANGGGDADGGAAVGISNSFGFGG
                     HNAVICLAGGER"
     gene            3839..>4895
                     /locus_tag="VF517_12800"
     CDS             3839..>4895
                     /locus_tag="VF517_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8053865.1"
                     /translation="MTRGFEMSRVAFDTFREGLPETRFDARQRIELLCDPGSAKVIRS
                     SVESRRIGSRARAGDGVVAAAGTVDGRPVFCFAQDAGFVGGSLGEAHADTIVRVLSLA
                     REARVPVVGFIESAGARMQEATAALGGYGRIFRETIALSDCVPQISIIAGTSAGGGSY
                     APALTDFVVMTKEARMFLTGPAIVRAATGEDTDAATLGGQAVHARNGVSHFVVPTDID
                     AAFLTRELLSYLPQNTSESPPDAEPHGPIGDNPGASVPRETRRVYDVRGVVRAIVDAG
                     RMLEVAPRWARNMVTGFARLDGRAVGIVANQPWHLGGVIDSDASEKAASFVSTCDRFR
                     VPLVVLVDTPGFLPGLRQ"
BASE COUNT          646 a         1731 c         1846 g          672 t
ORIGIN      
        1 cggtcgccgg cgacctgtac gtgatcgctc ccgacgacat gcacgacggt cgcgacctgg
       61 ggacgacgcg cggctggatg ctcggcttca tgcccgacgc gctgctgccc gactcgccgt
      121 ccacgcccgg ctacacgccg cttcccggcg acccgcgctg gcttgcgctg cggcgcaccg
      181 gggcccgccg cagcgctcgt tacgtggtcc ctccgcgcga tcgggggcgc tggtcgcagc
      241 gcttcgccga gatacgccac gagcttcggg cccggcggct cggctaccag cacgcggtgc
      301 gcgcgcacct cggactcctg ctcatcgcca ccacgcgcgc ggccatgttc gacggccccc
      361 cggtggaccc gacgccggac cccgtgcagg agatgatcga ggtgatcgac gcgcgctacg
      421 ccgagccgct gtcggtcggg gacgtcgcgc aggccgtggc cagctcgccg agtcacctcg
      481 gccggatggc gaagcgcgtc acgggggtga gcatccgcca gtggatcgag gagcgtcgca
      541 tgcaggaggc gcgtcgcctc ctattggaga ccgaccagtc gatcgacctg atcgcgcgga
      601 cggtcggctt ccacgacgtg cagtactttc ggcgccggtt ccggagggct cacggcatgc
      661 cgccccagac atggagggag cgcaaccgct gaggcgcgcg aatccggcga ctcaccgatc
      721 aggacgagcc tgacgcccaa acgggtggct cggtgcgcga aacgtccccc cacacgcgcc
      781 gttgtgacct gtaaaagcgc tgaagcggta cggataattc gcaatgtgcg gataacgagc
      841 acgacgaact cgaacttcga cgccgaggcc gggctgcccg ccgaccagca gcgccggctg
      901 cgcgtcctcg tcgtcgacga ccacgacgtg gtccagtggg gctttcgcat gctgctcgga
      961 tccgagtcgt gggtccagcg ctgcatcgcg gcgcgcgagc ccgaggaggc gctgcggctc
     1021 gcgactcgat acgagccgca cgtcgcgctg atagacgtac ggctgggcga ggagtcgggc
     1081 gccgagctgt gcacccgtct gatcgaggtg tcgccgatga cccgcgtcct gctcatgtcg
     1141 ggcttcgggc aggtcagcgc gagcgtcgcc cgcggcgcgg gcgcgcgcgg cttcgtgccc
     1201 aagagctggg acgcgcacga catcgccggc gcggcgcgca tggtcgggct cggcatgacc
     1261 gtgttcgtcc ccgttcagga gcagcccgtc cgggggctca ccgaccgcga gcaggaggtc
     1321 cttccgctga tcgcctcggg ggccaccaac cgcgagatcg ccgcgagcct cttcctctcg
     1381 accaacaccg tcaaggacca cacgcgcacc ctctaccgaa agcttcgtgc caagaaccga
     1441 gccgacgcga tcgtccgtgc gcagcggatg ggattgctcg attagctgcc gcgccaccgg
     1501 cgaggggtga ttacttcacc tcaacaggtg gatgcggtga ccggaggcgc cgggtgccgg
     1561 acgtcgcgtg cgagtctcgc gccggggagt gatccgaggc gaatcgtgac tgaggctggt
     1621 ctgggcacct gggcgggacc ggtgagaccg gcccgcgccg tccgcggggc cgcgatcgcg
     1681 tcactcggcg ccgccctgcc gcgcaccacg gtcccgaacg accagatcgc cccgcgcctc
     1741 ggggtggacg atcgctggat cctcaagcgg atcggggtgc gcgagcgcag gatgatcgcg
     1801 cccgacgagc ggctcagcga cctggtgacc gcggcggccc gccaggccct cggacgcgcg
     1861 gaggtcgatc cggaggatct cgacctcgtg ctcgtcggca ccttcacgca ggacgaggtg
     1921 ctcccgaacg ccgcaccgct ggtggcggcg gcgctggggg cgacgcgcgc cggcggcgtc
     1981 gacgtaggcg ccgcctgcat gggattcctg tccggcgtcg ccctcgccgc cggcatcatc
     2041 gaggcgggtc gcgccgacag cgtgctggtc gccggcgccg aggtgctcac gcgctacctc
     2101 gacgcggacg atcgtctgac cgccgcgctc atcggcgacg gcgccggcgc cgtggtgatg
     2161 acggccgtgg gtgcgccgag ccggatcggg ccgtgcgtgc tgcgctgcga cgaccgctcg
     2221 cgcgacgtcg tctacatgac ccgcaacgac atgaagctgc ggatggaggc gcagcagacc
     2281 ttcgtccacg cggtcgacta cctgtgcgac gtcacccacg aggcggtcgc cgccgccggg
     2341 ctcacgctgg acgagatcga cctgttcgtc taccaccagg cgaacgcgcg catcacgacg
     2401 tcggtcggcg atcgcctcga gcttccaccg gagcgggtcg tcgactgcat cgagacgctc
     2461 gggaacacgt cggcggcctc gatcccgctt gccctcgacg tggctcagcg cgaatgccgg
     2521 ctgcaccccg ggatgcgggt gctcgtggcg gcgatcggcg cgggcttcac ctggggggcg
     2581 gcggtgatcg agtggagcgc gggcggggtt cgttgaagcg gcgggtcgtg ataacgggca
     2641 ccggcgcggt aacgccgctc gggctggggt cgcgcgcgct catcgagcgc tgggtcgagg
     2701 gcaccagcgg tatcgaggac ggcctcggac gctgctcgga gttcgacccc tccgaggtgc
     2761 tcaccgtcaa ggaggtccgg cgcagcgacc gcttcacgca gttcgcgatc gccgcctgcg
     2821 cggaggcgct cgagtccgcg ggctgggcgg acggcctccc gtacgcgtcc gaccgggtcg
     2881 gctgcgtggt gggtacggga atcggcgggc tcgcgacgat cgaggagcag cacagcaggc
     2941 tgctcgacgc cggcgagcgc tccgtgtcgg cgctcggggt gccgatgatg atgtcgaacg
     3001 ccgcggccgg cgcgctcgcg atgcggcacg ggatgaccgg cccgagctgg ggtgtcgtgt
     3061 cggcctgtgc cgccggcgcc catgccatcg ggaccgcggt gcggctggtc cagacgggcg
     3121 acgcggacgc ggtcgtgacc gggggggccg aggcggcgct gacgccgatg gcgcgggccg
     3181 gcttcgcccg gatgggcgcg acctcgccga ccggcgtctc gcggccgttc gacgcgcgcc
     3241 gcgacgggtt cgtgctcggc gagggcgccg ggatcctggt gctggaggag cgcgaggccg
     3301 cgctcgcacg cggcgcccag ccgatcgggg aggtcctcgg ctactgcgcc accgccgacg
     3361 cctaccacct gaccgcgccg cagccgacgg gcgagtgcgc ggcgcgcgcg atagccggcg
     3421 cgctcgcgga ggccggcctc gacgccgcgc gggtcgacta cgtgaacgcg cacgggacgt
     3481 ccacgccgct gaacgaccgc accgagaccc aggcgctcaa gtccgcgctc ggcgaccggg
     3541 ccgccacggt ccccgtgtcg tcgctcaagt cggccatcgg ccacctgctc ggcgcgagtg
     3601 gggccgtcga ggcgatcgcc acggcgctgg cgctgcgcga gcgcgtcgct ccgcccacgc
     3661 tcaactggtc ggagcccgag gaggggctcg atctcgacta cgtgccgggc gtggccaggc
     3721 ccctgcgcga gcgcgcgaac ggcgggggcg acgctgacgg cggggccgcc gtcggcatct
     3781 cgaactcgtt cgggttcggg gggcacaacg cggtgatctg cctcgccgga ggggagcggt
     3841 gacgcgtggc ttcgagatgt cgagggtggc cttcgacacg ttccgggagg ggctgccgga
     3901 gacgcgcttc gacgcccggc agcggatcga gctgctgtgc gacccgggat cggcgaaggt
     3961 catccgctcg tcggtcgagt cgcggcggat cgggagccgg gcccgcgccg gcgacggcgt
     4021 ggtggcggcc gccgggacgg tcgacggcag gccggtgttc tgcttcgccc aggacgccgg
     4081 gttcgtcggc ggctcgctcg gcgaggcgca cgccgacacg atcgtgcgcg tcctctcgct
     4141 cgcgcgcgag gcgagggtgc cggtcgtcgg cttcatcgag tccgccgggg ctcggatgca
     4201 ggaggcgacc gccgcgctcg gcggctacgg ccgcatcttc agggagacga tcgcgctgtc
     4261 ggactgcgtc ccgcagatct cgatcatcgc gggcacgtcg gcgggcgggg ggtcgtacgc
     4321 gcccgcgctg accgacttcg tggtgatgac caaggaggcg cggatgttcc tgacgggccc
     4381 cgcgatcgtg cgtgcggcaa cgggcgagga caccgacgcc gcgactctgg gtgggcaggc
     4441 ggtgcacgcc cgcaacggcg tcagccactt cgtggtgccg accgacatcg acgcggcgtt
     4501 cctgacccgc gagcttctgt cgtacctacc gcagaacacg tccgagtcgc cgccggacgc
     4561 cgagccgcat ggaccgatcg gcgacaaccc gggcgcgagc gtcccgcgcg agacgcggcg
     4621 cgtctacgac gtccgcgggg tcgtgcgcgc gatcgtcgat gccgggcgga tgctcgaggt
     4681 ggcgccgcgc tgggcccgca acatggtcac cgggttcgcg cgcctcgacg ggcgcgccgt
     4741 cggaatcgtc gcgaaccagc cgtggcacct cggcggcgtg atcgactccg acgcgtccga
     4801 gaaggccgca agcttcgtgt ccacctgcga tcgcttccgc gtcccgctcg tcgtcctcgt
     4861 cgacacgccc ggcttcctac ccggcctgcg ccagg
//
LOCUS       DASVPL010000240        29226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_28418, whole genome shotgun sequence.
ACCESSION   DASVPL010000240 DASVPL010000000
VERSION     DASVPL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 29226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29226
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28418"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="VF517_12805"
     CDS             complement(<1..1476)
                     /locus_tag="VF517_12805"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX8053866.1"
                     /translation="MATAQKAPARRSTRAKTSNGKDGAPPKRRADDNGAIDAGDLENL
                     LEGLHAARHGDFSRRLSTRRRDMVGDLARAYNELVEQNARMTKELSRVGRVIGREGRM
                     AERASLPGSGGDWESSIDSINDLVDDLVRPTTEVARVIDAVADGDLSQKMALKIEGQP
                     IRGEFLRIGTTVNTMVDQLSSFADEVTRVAREVGTEGKLGGQAEVKGVSGTWRDLTEN
                     VNSMASNLTEQVRSIAHVTTAVANGDLSQKITVEAKGEVAALAMTINTMVGQLSSFAD
                     EVTRVAREVGTEGKLGGQAQVEDVSGRWRDLTESVNFMASNLTSQVRNIAQVATAVAE
                     GDLSQKITVDARGEILELKDTINVMVDQLRAFAAEVTRVAREVGTEGKLGGQAQVEDV
                     SGTWRGLTENVNLMADNLTYQVRNIAAVTTAVAQGDLSKKITVDARGEILELKETINT
                     MVDQLRSFAAEVTRVAREVGTDGILGGQAEVEGVSGTWRDLT"
     gene            complement(1580..2617)
                     /locus_tag="VF517_12810"
     CDS             complement(1580..2617)
                     /locus_tag="VF517_12810"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8053867.1"
                     /translation="MARVLTQRELGRATLARQMLLAREEIGAVAAVERLAGLQAQVRE
                     PPFTGLASRVDGFERAQLIEAIERREVVRATAMRCTMHLMSARDFLDLWGAIRPAIAR
                     AVRGWTGKRLAGLDMDAVAAEARAHFHERPRTFAELRDLIPTIADTDEPQAMAYAVRS
                     HLPLVQVPDGGSTGHSNQAPYGLAEDVLGEPAVTDPDPAELIRRYLAAFGPASVADAQ
                     AWSGVPGLKPAFEALRDELVTFEDEGGRELFDLPDAPRPPADTPAPPRLLPEYDNLVL
                     AHADRTRFVAEEHRKAIYLTAGRVRATFLVDGVVAGVWDPRARELEPFGRLQRSDRAA
                     LGRELERLGDA"
     gene            2636..3073
                     /locus_tag="VF517_12815"
     CDS             2636..3073
                     /locus_tag="VF517_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8053868.1"
                     /translation="MARARPVGINHVALTVGDLDEALAFYGRIFEFELRGRAPGTAFL
                     DMGDQFLALAEGRDAPPDAQRHFGLVVDDREAARRALEEAGAEIVPGRALRFRDPWGN
                     QVELVEYGDVQFTKAPGVLGGMGLGDLRKSAGALRQLEEKGLG"
     gene            complement(3074..3619)
                     /locus_tag="VF517_12820"
     CDS             complement(3074..3619)
                     /locus_tag="VF517_12820"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEX8053869.1"
                     /translation="MKTKPIFASIPLPEGMPPTFAASRSYMHWYLEGIVDRKLRSDFV
                     VEAEVVVYTGAAGAAPQGVGGQPGMAQAGGAPGGAGAPQPIAPGFGEPVEMGPPGGAA
                     GGYGQPGGMQLPGGVQADPAGFQQPAGPQPIASPPAPAGGAPSAEALPGSFAADWYPD
                     PWLQSRLRYWDGNAWTGHLAE"
     gene            3603..4058
                     /locus_tag="VF517_12825"
     CDS             3603..4058
                     /locus_tag="VF517_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8053870.1"
                     /translation="MGFVFTRRGIVPEACSSWFLPRIVGIGQAMEWVATGRVFPAAEA
                     LAGGLVSSLHPKDELLGAAHALAREIADNTAPVSVALGRRLLWTMLGAEHPMEAHRAD
                     SRAMFARGQSADASEGVTAFLEKRPAEFPDRVSDGLPEIFPGREVPAFR"
     gene            complement(4059..4610)
                     /locus_tag="VF517_12830"
     CDS             complement(4059..4610)
                     /locus_tag="VF517_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053871.1"
                     /translation="MRRGLAALMIALAAVLVAVPLGIAATSGGTDERGDVEGSPSGGA
                     DRADIVRATAGRTSDGQIKHTVTVAGSAADPASGDLATYIYIEAQTDAGAMDECTFFV
                     GRYHGRLGVYLCGTAERLGSARVVRTSEHTTRYTFSPRAIGNPKTYQWAAITRGVTNA
                     RGTFTRHDRLPSGDEFFFEQKLP"
     gene            complement(4692..5150)
                     /locus_tag="VF517_12835"
     CDS             complement(4692..5150)
                     /locus_tag="VF517_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053872.1"
                     /translation="MEQDVRTPIERRIQRSLEDDTGHGKPLSRRTRQTKRTVEAYLAA
                     GVMPRYMERLAEIDSSLKIQRGRLERAYLALKEACGEDREAFGRQWRARLDAWPFDHL
                     NALIREHNEWYPMESGLPLDPRTGDYVRRAGRSYRREEIGPAWALEQFPA"
     gene            complement(5161..6432)
                     /locus_tag="VF517_12840"
     CDS             complement(5161..6432)
                     /locus_tag="VF517_12840"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX8053873.1"
                     /translation="MGGVDRRTFMAATAVTAANPQVALAKRKPAKPPLGAGGAPMPLD
                     ALAKLARDHGRGQPGLFLDLAALDQNTRTVVDFAKAQGWGVRPALKSFRSPALISYLL
                     NRLPEPRGLVFNLSEVDPIIAQAPRNTDLMTGYPPTFGELQTFLRTKQPRGQRPHRLR
                     ILIDSVAVAEHLATLARSARRPVPEVALEFDVGMGRGGINDADEMRACLEALRELKLT
                     AVLGYDGHATLRGDPEYRRLVATQAQEVYRTRLAELKALGHEPEVRNGPGSSNYRNWT
                     GGPANEIGCGSAFLYAAYLNGGYDTDGLAPALTLGGAVRRITSDHPSVPVIGVAQPGA
                     AEMEIVVQGVAAASELAHPPGGREDEASGGGDALIVPKGSVALGDYVLYRPTQTEGAI
                     LGYHELVAVREGRLLRRWPVFKRQGARDLTP"
     gene            complement(6445..7365)
                     /locus_tag="VF517_12845"
     CDS             complement(6445..7365)
                     /locus_tag="VF517_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053874.1"
                     /translation="MRHKIRTPREERLAALAARASRQYGILAIWQLSELGFTPDSVKR
                     LVAARRLHRLYRGVYALGHRSLAPRGRVLAAVYAYGPDAVASHLAAAWVHDLRRSRGS
                     VVHVTVVGKERRARAGTRLHAVRTLHTEDVTVVEGIPVTSVARTLTDLGASLSEKQLR
                     RTFEEADRRGLLDASAVRKACERGRGRRGIGNARVALAEYRPDSPKTRSDLEIEFLEF
                     CRANGIPEPSVNVWVEGQEVDMAWLDRGVVVELDSYEYHGTRAAFERDRVRSAELEVA
                     RWRPIRVTSQRMQQATALRSHLLSLLAVSD"
     gene            complement(7416..9017)
                     /locus_tag="VF517_12850"
     CDS             complement(7416..9017)
                     /locus_tag="VF517_12850"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HEX8053875.1"
                     /translation="MTTTLEPTRSAPLDTGSKRVTGAHLAALAARVSAGEGERETIEI
                     ENPSTGSVLATVPRCTAEDIDAAVARARVAQARWRESSFEERREILLRFHDLVLDRQD
                     EILDVIQLESGKARRHAYEEILDAAVVARYYARSAERHLREQRRQGALPVLTATWEYH
                     HPVGVVGVIAPWNYPLTLSISDAVPALAAGNAVVMKVDTQTPFSALIGLALLEEAGLP
                     SELLQIVTGSGAQLGPELISRVDYVMFTGSTAVGRQVAAQAAERLVPSSMELGGKNAM
                     IVLEDADLTRTVEGAERALFSNAGQLCISVERLYVHESIADEFTRRLVERVEKLKLGN
                     SLDYSCDMGSLISAKQLETVREHLEDAVAKGARVLTGGNARPDIGPYFHEPTLLADVR
                     DGMTLFRDETFGPVASISTFSSEEDVVARANDSDYGLNFSVWSSDVDRARHLAVQLQA
                     GTVNINEAYIATWGSVDAPMGGMKDSGMGRRHGADGILKYTESQTVSVQRLLPIAPPP
                     RVGQRVWTKAITLGLRALRRVPGKE"
     gene            complement(9017..10360)
                     /locus_tag="VF517_12855"
     CDS             complement(9017..10360)
                     /locus_tag="VF517_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8053876.1"
                     /translation="MTTTTEAPPAALPSASVADSTKVLVAGVVPAITRGLFKPRRRAM
                     KRLTALDADRRTVETLAAIKRKHPGQGARLLGGRMVVLWGADAIREVLDQSADVYAAD
                     AGAKQKGMCHFQPDALTISRGEEWRDRRAFTESVLATAERVHPLGDRFLSVVADEMSR
                     MRISGRVEWERWERLFDHVTLRVIFGEKARGDQDLTALLSELMCEANRIVAREAESSD
                     AYHELHGRMEWYLRDPEPGALVARFADAPQTDRTRVVHQIPHWVFAMRDTLAANAYRA
                     LAVIAADEAVEERVREEMDGRDLTSAADVDGLRYLEGCLQETMRLWPTTPLIAREATR
                     DTTLAGERIDEGTQVLMVNAFNHRDPDGVADADLLKPERWESSQRDYRFNHLSNGSQW
                     CPGEPLVLLLGKAVLAQMLAAWDVTVLEPELPAGSKEPLPHSLDFYSIRYAVRRR"
     gene            complement(10357..12240)
                     /locus_tag="VF517_12860"
     CDS             complement(10357..12240)
                     /locus_tag="VF517_12860"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX8053877.1"
                     /translation="MKRSLGGRALHGVFGRINERKQWHELPFPINLLNLLSLRLDLRD
                     MNLHDTTPVYEAGPEQEPPEEYRAARHPEGKWNDLVQPDMGSTGTAFGRNIDTRRIKP
                     EKPPRIHDPSARTVSLELMTRDSFKPATTLNILAAAWIQFENHNWFFHGRGEPDNTMD
                     VPLEPGDDWHEDPMRVRRTVSVPSGEDNGDGSVNFGNTETHWWDGSQLYGVGIEKQNQ
                     LRTFQDGKLKIGDDGRMLPDPDEDGVDLTGFNENWWFGLSCLHTLFTKEHNAICDALK
                     KENPRWSDQRLFDTAWLVNSALMAKIHTVEWTPGIVNNTALWAAMNANWSGVLGQHTK
                     DLIGRLGDSEIFSGIMGSPAEHHEARFQLTEEFVSVYRMHPLIPDDWKIYDFGSGSVR
                     EEREFTELATKNTRSFMDGYELTDLFYSLGVEHPGAITLHNYPRALQRHVRIDGEVMD
                     LATLDILRDRERGVPRYNDFREEMRMPRVRSFDELTPNKVWAEQIRRVYDNDIDKVDL
                     QIGMYAEPLPPGFGFSDTAFRVFVLMASRRLKSDRFFTTDFRPEVYTELGIRWVNRTK
                     MKDVILRHYPELEPALEGMPNAFLPWHRVEQFKGGSEGDGRVTSAKKRVEGIQKAMEA
                     INR"
     gene            complement(12318..13934)
                     /locus_tag="VF517_12865"
     CDS             complement(12318..13934)
                     /locus_tag="VF517_12865"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX8053878.1"
                     /translation="MICRGLTLLIVVLAMCVPAHAAAAPDPFFGTLFDDNAALTREQR
                     QQDMDRQAAAGIGTIRHHVFWSQVETEWTNASATGVYDWRALDQIIGDAADRGLRVLP
                     VVFSTPEFYKPDGARTDRQVPPADPVYLGRFLRKLAERYGPNGSFWCSGLPRTCRSAV
                     LPIRAWQVWNEPNLPAWWKGDSEPDPAEYVELLKHAQLNLRLADPGAEVVMAGLAPSF
                     TTPSRQDFRVYLNGLYDAGAAAYFDALAIHAYRQDVEGVVEIPRMVREIADAHGDTAT
                     PIWITEWGWASGGANSPFVTTPSCQAARVHAAAGAWRALRDELRLSAAIQFQWRDVAT
                     TRTAWPFHAGLNPASLSEPPKPSLGQYANAIAGRPPDSGYDVATACSPAPPPPPAAGP
                     PAGAAVNPGADPPPSDAAAQHAVPPPPTTSAAADADLTPPDPRIVAATLRGGRLLMTV
                     GCGAEPCTAIASASLRLPGRAFRLTGATRAVPAGGTATVRLALPEKARRALKQPRLRA
                     RARARIALRALDPAGNSATRKRTLALAPTR"
     gene            complement(13973..14343)
                     /gene="ssrA"
                     /locus_tag="VF517_12870"
     tmRNA           complement(13973..14343)
                     /gene="ssrA"
                     /locus_tag="VF517_12870"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(14343..14825)
                     /locus_tag="VF517_12875"
     CDS             complement(14343..14825)
                     /locus_tag="VF517_12875"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8053879.1"
                     /translation="MPLKARASARNGSDSTDRLILDELQREGRVTFAELGRRVGLSPP
                     AVAERVQRLEDDGTILGYGALVDPRAVGYSLSAIIRVRPAPDRLKTVADVARKTAEVV
                     ECHRVTGDDCYVMKAHLRDVEHLEEVIDRFAALGQTTSSIMQSSPVPQRPLATTIERT
                     "
     gene            14891..15769
                     /locus_tag="VF517_12880"
     CDS             14891..15769
                     /locus_tag="VF517_12880"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8053880.1"
                     /translation="MRREPPPQVYFVFSAVFHYLGPAFAVLLFARVQPLGVAWLRIAS
                     AAAILGLWRRRIVLSRLVVAWGAVLALMNCCFYVAIDRLPLGTVAAIEFLPVIVLAAA
                     GARTVRNGAALALAVPGVYVLADVRLEGEPLGVALAFANALLFALYIVLAHRVAQHAS
                     IGGIDGLAASMLIAAIVVTPMAGWAAVPALTDPVALAAGVGVGVSSSVIPYIADQLAM
                     ARLTRAAYALMVSLLPATATVIGVIVLAQIPSAREAAGVALVVAGVALHREARPARNE
                     SARGQHRAEHEQPASA"
     gene            complement(15792..16484)
                     /locus_tag="VF517_12885"
     CDS             complement(15792..16484)
                     /locus_tag="VF517_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEX8053881.1"
                     /translation="MKGRGLALACATTAMWLVMAGSALAAKPGGGGGTTTTPYDPILF
                     VHGWNSSGTVWTDMISRFQSAGWPSSSLNNWSYNTAQSNATTAAQIRDKVDAILAASG
                     ATKVDIISHSMGGLSSRYYVKNLGGDLKVDEWVSLGGPNHGTDTANFCFDTSCGEMRI
                     GSSFLTALNSGDETPGTPRFGTWWSPCDEVINPDSSVALGGATNTQTACLSHSGLYGD
                     ATVFGQVRDFVR"
     gene            complement(16577..17464)
                     /locus_tag="VF517_12890"
     CDS             complement(16577..17464)
                     /locus_tag="VF517_12890"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8053882.1"
                     /translation="MSDEPREDEAAEVEGEAAEVEAGTDGELAEVEAELEAADAEGGR
                     LAALAKLRLADGSPRVLNAARAVRRLLPGDSELGDPLSMAGDDPSLVLARRVAEVGQQ
                     RPSALRELGLGAFQVWQAISEKQGRGRGDRELAILFTDLVEYSTWALEAGDEASLALL
                     REVGRAEDSAVAEHGGRVVKRLGDGLMAVFNDPQEAVLAACDVRERVAAIEQDGYRPR
                     LRAGVHLGRPRRIGGDYLGVDVNIAARVADAASGDQILISGPTCDRLEDGAFKCKRQR
                     RFKAKGAPKGLEVFAVEPA"
     gene            complement(17484..18476)
                     /locus_tag="VF517_12895"
     CDS             complement(17484..18476)
                     /locus_tag="VF517_12895"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053883.1"
                     /translation="MPVVFARSPLRVTLAGGGTDLPSWYGRNGGYVVAAAIDRHVFML
                     VNTEHQDRIRLKHMEWEEADAPEQVQHPILREALLRRWNGAPLELASTSDVPPGTGLG
                     SSGAYAVCALGALALAGGSPAAAAELAEEACDIEIGVLRRTVGKQDQYASAFGGIRAY
                     GIARDGVVAAEPLALDEAVIGALDDGLLLFYSGEQRSASDLLAGEAQAAQAGDGTVER
                     SLGRAVEIAHAVRDALERGDLGAFGALMNEQWDAKLARAPGMATARMDELRRAALGAG
                     AEGVMQMGAGGGGYLLAYAADPERVRASMETAGAPELRFRLDPDGFRGEAFVPA"
     gene            18527..18949
                     /locus_tag="VF517_12900"
     CDS             18527..18949
                     /locus_tag="VF517_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053884.1"
                     /translation="MDDLLRPSLQEHEPAQADAAKPWRVSSQVYVGFFGGALAVGLIA
                     FLNTYRLRCPDRSRLLVVGATLAGCAATATMLVLGIERPSFQVGGVTAYGLVYLIQRR
                     ADRAFEIFDGDHESLVRPGIAAALAGWFLSLNVIVWAS"
     gene            18949..19818
                     /locus_tag="VF517_12905"
     CDS             18949..19818
                     /locus_tag="VF517_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8053885.1"
                     /translation="MRTDADNSALRMAEYYLEINRPEEALHALDSSGVALEEPGAWLI
                     RGTALFALERHDEAGKAAEEGLREDPDDVWLLHLLSIAKEALGDLAASERAILAALEL
                     EPDIPGLYTQYADLLMRAGQLDKAHAVIERAAALDPEDLSVLRARMSYAYVTGKAREG
                     EKWSRKMLELEPEHPGAHGMLGVFKIEQGSVIGAREHYETAVALDPSSPDAREATIEI
                     RTATHPAMLPARALDRLGVAGSWVAAVIVITALRSVSPGAGAVAGITWVTFCVYTWVA
                     PPIVRRLVIRKLS"
     gene            19815..21089
                     /locus_tag="VF517_12910"
     CDS             19815..21089
                     /locus_tag="VF517_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8053886.1"
                     /translation="MTDRIEGLRAALEASPDNAALRAVLAETLADAGQVDEALDHYDR
                     LLEARALSGEQALVAGRLALAAERYQIAARCLEHAKSQGALEGVADLQHDLERGLSAG
                     GDPPPRIERSPATPPELQPDELLDEERRITFADVGGLAEVKKTIHRTIILPFQRPDLY
                     LKYGRRAGGGVMMFGAPGCGKTMLARATAGECGLPFFNVRIEDILDPHLGGSEEQLSF
                     AFETARASAPCVIFLDELDALAYARRKQQGFGRSLVNAILQQLDAIGSDNRDLLIMGA
                     TNAPWDVDDGLKRPGRLDRVLFVPPPDEEARARILELGMRDRPTEGIDIRALVKATPL
                     FSGADLRALVEQATDEVIDEALDSGGEPPMRMAHLEKVLREMRPTTLDWLASARNYVQ
                     FANQSGRYDEVAAFLRSRDAKKALKEIAKHDE"
     gene            complement(21160..21615)
                     /gene="smpB"
                     /locus_tag="VF517_12915"
     CDS             complement(21160..21615)
                     /gene="smpB"
                     /locus_tag="VF517_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX8053887.1"
                     /translation="MPPQRDIARNRNASHRYNLLDKWECGIQLMGTEVKSVRAGRVQL
                     KDGYAAVEEGEVWLQNVHIAPYDPARDNHDPERPRKLLLHRREIERLVGKVSERGLTL
                     VPTRMYFKGKHAKVEIALASGKDLYDKRRSLKEKDMKREIEREMGTRRR"
     gene            complement(21626..23299)
                     /locus_tag="VF517_12920"
     CDS             complement(21626..23299)
                     /locus_tag="VF517_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX8053888.1"
                     /translation="MVASPVFERGRHVSLDSAKGAGVGDLVLLGAGKRGVRVVRRLGR
                     PDVARDVLEALMIERGLRRSFPRAVEGAAEDAAQDPPANDAPRADLTGLATFTIDPAT
                     ARDYDDAVSATREDGGIIRVWVHIADVSAFVRPGSVLERETFRRATSVYVPGAVEPML
                     PHALSSGACSLVSGQDRLAVSVELEMDGAEVRSAAFHRSRIRSDARLTYPQVDRIFAG
                     SERAEEPWGEPLALAREVSAALREKRERRGSLAVESAEPVFEFDDRGHVVGVGHEEQT
                     ESHKLIEELMILANEQVAGQLADRRVPTLYRVHEKPDPRGVERLAEQLESLDVPTPPL
                     PEGMSPQQAGDAAAEISHVIARYVRKSGRGKRSFGTLVLRAMKQAYYSPRNIGHAGLA
                     SERYCHFTSPIRRYPDLVAHRALLATIGADDTPPRADELFDAAEHSSTAERDAMKIER
                     GADDVCMAFLLERRLAEAGWDLELDGEVVGVISKGLFVKFGDEGFEGFLPVRRMRGDW
                     WEINEQETALVAADSGRRIRLGDPLRVAVGRVDAPRGRADLELAADAAH"
     gene            complement(23359..24534)
                     /locus_tag="VF517_12925"
     CDS             complement(23359..24534)
                     /locus_tag="VF517_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8053889.1"
                     /translation="MRSALTAFFACATVIVVFVGGLWLGGHPGKLPDPLRDAFVEDRR
                     SVRAELLESIQDDYYKKVDTDKLEGQELKGIVKALGDRFSAYYPPDEAKEFRQALSAE
                     FEGIGVTVQPNDKGLEVVSVFEDTPASRAGIHKSDLIVGVNGKSIAGVSSDVATGRIK
                     GKAGTKVKLSVLPAGEKKPRDVDVERAKIDVPLAKGRMEKSGGRKIAYVRLAAFSEGA
                     HAAVRKELEPLLKQGADGIVFDLRGNGGGLLTEAVLVSSLFVEDGLIVSTKGRNRAER
                     KFDAEGSALAPDLPMAVLVDRGSASASEIVTGALRDKGRATVVGEKTFGKGVFQEVEF
                     LSDDSILELTVGSYYLPKGENLAQKGIPPQVRARDLPRTRRDEALPVAVDRVARTAR"
     gene            complement(24556..25452)
                     /gene="ftsX"
                     /locus_tag="VF517_12930"
     CDS             complement(24556..25452)
                     /gene="ftsX"
                     /locus_tag="VF517_12930"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX8053890.1"
                     /translation="MTRVGFFLKEAFRALSRNAAPSLAALITIVLTALVLGVFIPVVQ
                     ATTGTANDVRERVVVDVFLKQGSTDAQRTQLRQALLATPNVRSVEFVSKQEALKEQTK
                     RNKQAFELLGSNPLPDAFRVSPRDPEGVNAIVDRIAPRGPSGERTSRLAAVDEVRNRE
                     RDTDKILDATGLVKALTASLAALFVFASIALVANTIRLSLFARRREIEVMKLVGATNW
                     FIRWPFVIEGIVVGFMGGVIAALMLLIAKETLVDPLSDRFALLAAPDTIDFPLLVAML
                     MAACVLVSSLGSGITLRRFLRV"
     gene            complement(25456..26397)
                     /gene="ftsE"
                     /locus_tag="VF517_12935"
     CDS             complement(25456..26397)
                     /gene="ftsE"
                     /locus_tag="VF517_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX8053891.1"
                     /translation="MAVVESENVRPVRTDAERPYVPVVELREVTKVYEGGSLAVDRVS
                     LQVTRGEFVFLVGPTGCGKSTLTRLLMKELEPSRGEVLIAGRKLGEIPPKKVPKLRRN
                     IGVIFQDFKLLPNRTVYQNVAYSLQVIGENRQQIRKKVPEILRLVGLSTKLHSYPDQL
                     SGGEQQRVSIARAFVNHPPLLLADEPTGNLDPETSIGIMQLIYRINRTGTTVVVATHD
                     REMVDKMRRRVIELSEGRVVRDDESGLYRAEESTAEFAMRVREELGYGKETVTRVKRD
                     LEPDHVPLDARHRHFTDGDRDRYAEEKVRRALYGEDL"
     gene            complement(26463..27221)
                     /locus_tag="VF517_12940"
     CDS             complement(26463..27221)
                     /locus_tag="VF517_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8053892.1"
                     /translation="MEFQSLFPSTGTLTATEAAAELRAGARPHASRPYLVLNMVATAD
                     GRITIDGRSGPIGNAHDRALFHELRAQVDAVMVGAGTARNERYGRLVRDPDRRDRRVA
                     AGLEADPLAVVVSARLALDPENVPLLQDADSCVTCVTAADETIAGVLASVDYIRTPKD
                     EAAGIVPMRPALEELRERGVETVLCEGGPVLNGTLLREGLVDELYLVVASKLAGGGGP
                     SVVSGPELDPPTGLELVSALEGGGDLFLRYRVAP"
     gene            27326..28681
                     /locus_tag="VF517_12945"
     CDS             27326..28681
                     /locus_tag="VF517_12945"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8053893.1"
                     /translation="MTPRRLIEPARLAGSALLHTQSDERLVDLARSGNDRAFEAIVAR
                     YRRPLLRYCRKLLPPERAEDAVQQAFLNAYRAIGAGEAELNLKPWLYRIAHNASLSLL
                     RQNGWSHEELDEQFDGVQQPPQAFEQREQVREVVRAVQGLPERQRDAVVLRELEGRSY
                     DEIAVALGVSGGAVRQLLNRARTNLRAAATALTPFDLVAWCVSRGGAGATGAERIAEL
                     TAGGAGGLAAAAKLGTALVVAGAVAGAGTGVGPLPDMVGGGGDAGAEEAAAAEPAGGP
                     AGEDGPATRVRHGRGADGRTEGRGRRGGDDRAGRDRSRSGSDDDSGSRGSGSGGGSDD
                     DDGGSGSSGSGSGDDDSGSGSSGSGSGGSDSSGSGSSGSDSSGSGSSGSGTSGSGSSG
                     SGTSGSGSDSSGSGPGPDSRSGSGDSGTSGSGSGTSGSGSGSGSNSGSGSGLSSGSGS
                     E"
     gene            28859..>29226
                     /locus_tag="VF517_12950"
     CDS             28859..>29226
                     /locus_tag="VF517_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053894.1"
                     /translation="MKRTILRGTVVAAALALMLPAAASASRGDRDGDKMSDRWEGKHG
                     LRVGKDDARRDPDRDRLSNLKEFRLKTNPRRADTDGDGLRDGDERRTRNDARDRDSDD
                     DGVRDGDEVNGTVASFDSATG"
BASE COUNT         3853 a        10524 c        10521 g         4328 t
ORIGIN      
        1 cgtgaggtcg cgccacgtcc cggagacgcc ctcgacctcc gcctggccgc cgaggatgcc
       61 gtccgtgccg acctcgcgcg cgacgcgggt gacctcggcg gcgaaggacc gcagctggtc
      121 gaccatcgtg ttgatggtct ccttcagctc gaggatctcg ccgcgggcgt cgacggtgat
      181 cttcttggag aggtcgccct gggcgacggc cgtcgtcacc gcggcgatgt tgcgcacctg
      241 gtaggtcagg ttgtccgcca tcaggttcac gttctcggtg aggccgcgcc aggtgcccga
      301 cacgtcctcg acctgggcct ggccgccgag cttgccctcg gtgccgacct cgcgcgcgac
      361 gcgggtcacc tcggcggcga acgcccggag ctggtcaacc atcacgttga tggtgtcctt
      421 gagctcgagg atctccccgc gcgcgtcgac cgtgatcttc tgcgagaggt cgccctcggc
      481 aaccgcggtc gccacctgcg cgatgttgcg cacctggctc gtgaggttcg aggccatgaa
      541 gttcacgctc tcggtcaggt cgcgccagcg ccccgacacg tcctcgacct gcgcctggcc
      601 gccgagcttg ccctcggtgc ccacctcgcg cgccacgcgc gtgacctcgt cggcgaacga
      661 cgagagctgc ccgaccatcg tgttgatggt catcgcgagc gcggccacct cgcccttggc
      721 ctcgaccgtg atcttctgcg acaggtcgcc gttggcgacc gcggtcgtca cgtgcgcgat
      781 gctccgcacc tgctcggtga ggttcgacgc catcgagttc acgttctcgg tcaggtcgcg
      841 ccaggtgccg gagacgccct tcacctcggc ctggccgccg agcttgccct cggtgccgac
      901 ctcacgcgcc acgcgcgtga cctcgtccgc gaacgacgag agctggtcca ccatcgtgtt
      961 caccgtggtg ccgatccgca ggaactcgcc gcggatgggc tggccctcga tcttcagcgc
     1021 catcttctgg ctgaggtcgc cgtcggccac ggcgtcgatc acgcgcgcga cctcggtggt
     1081 ggggcgcacg aggtcatcga ccaggtcgtt gatcgagtcg atgctgctct cccagtcgcc
     1141 gcccgaccct ggcagcgagg cgcgctcggc catgcggccc tcgcggccga tcacgcggcc
     1201 gacgcgcgag agctccttcg tcatgcgggc gttctgctcc acgagctcgt tgtaggcgcg
     1261 ggcgaggtcg ccgaccatgt cgcgccggcg cgtcgagagg cggcgcgaga agtcgccgtg
     1321 acgcgcggcg tggaggccct cgagcaggtt ctcgaggtcg ccggcgtcga tcgcgccgtt
     1381 gtcgtcggcg cggcgcttgg gaggcgcgcc gtccttgccg ttgctggtct ttgcgcgcgt
     1441 gctgcgccgt gcgggggcct tctgcgctgt tgccacgcgc cctccttcgg gtccgtcgag
     1501 gtaagtcctg ggttgcggca gcgtactccc gcgcgccgcc cggcccgccg ggtggaggat
     1561 ctgcacgggg tgcgccgagc taagcgtccc cgaggcgctc cagttccctg ccgagcgccg
     1621 cgcggtcgct gcgttgcagc cggccgaacg gctcgagctc gcgtgcacgg ggatcccaca
     1681 cgccggccac caccccgtcc acgaggaacg tggcgcgcac gcggccggcg gtgaggtaga
     1741 tcgccttcct gtgctcctct gcgacgaatc gtgtccggtc ggcgtgggcg agcacgaggt
     1801 tgtcgtactc ggggagcagg cgcggcgggg ccggcgtgtc cgcgggggga cgcggcgcgt
     1861 ccggtaggtc gaacagctcg cggccgccct cgtcctcgaa cgtgacgagc tcgtcgcgca
     1921 gcgcctcgaa cgcgggcttc agcccgggga cgccggacca ggcctgggcg tccgccacgc
     1981 tcgccgggcc gaacgcggcg aggtagcggc ggatcagctc ggccgggtcg gggtcggtca
     2041 cggcgggctc gcccagcacg tcctcggcca acccatacgg cgcctggttc gagtggccgg
     2101 tgctgccgcc gtcggggacc tgcacgaggg ggaggtgcga gcggaccgcg tacgccatgg
     2161 cctgcggctc gtcggtgtcg gcgatcgtgg ggatcaggtc gcgcagctcg gcgaacgtgc
     2221 gcgggcgctc gtggaagtgc gcgcgcgcct cggccgctac ggcgtccatg tcgagcccgg
     2281 ccagccgctt gccggtccag ccgcgcacgg cgcgggcaat cgcggggcgg atcgcgcccc
     2341 acaggtcgag gaagtcgcgc gcgctcatca ggtgcatcgt gcagcgcatc gccgtggctc
     2401 ggaccacctc gcggcgctcg atcgcctcga tcagctgcgc gcgctcgaac ccgtcgacgc
     2461 gactggcgag cccggtgaac ggcggctcgc gcacctgggc ctggaggccg gccaggcgct
     2521 cgacggccgc gaccgcgccg atctcctcgc gcgcgagcag catctgccgc gccagcgtgg
     2581 cccggccgag ctcgcgctgg gtgaggacac gcgccacggt tggcagacta cgagcgtggc
     2641 gcgtgcacga ccggtcggga tcaaccacgt ggcgctcacc gtgggcgacc tcgacgaggc
     2701 gctcgccttc tacgggcgga tcttcgagtt cgagctccgc gggcgggcgc cggggacggc
     2761 gttcctcgac atgggcgacc agttcctcgc gctcgccgag ggccgcgacg cgccgcccga
     2821 cgcgcagcgc cacttcggcc tcgtggtgga cgacagggag gccgcgcgcc gcgcgctcga
     2881 ggaggccggc gcggagatcg tgccggggcg ggcgctccgc ttccgcgacc cgtgggggaa
     2941 ccaggtcgag ctggtcgagt acggcgacgt gcagttcacg aaggcgcccg gtgtgctggg
     3001 cgggatgggg ctcggcgacc tgcgcaagag cgcgggcgcg ctgcgtcagc tggaggagaa
     3061 ggggctcggc tagctactcg gcgaggtgcc cggtccaggc gttgccgtcc cagtagcgca
     3121 ggcgcgactg gagccacggg tccgggtacc agtcggccgc gaacgagccg ggcagggcct
     3181 cggcgctggg cgccccgccc gcgggcgcgg gaggcgaggc gatcggctgc gggccggcgg
     3241 gctgctggaa gccggcggga tcggcctgga cgccgccggg aagctgcatg ccgcccggct
     3301 ggccgtagcc gccggcggct ccgccgggcg ggcccatctc gaccggctcg ccgaagccgg
     3361 gcgcgatcgg ctgcggcgca ccggctcccc ccggggcccc gcccgcctgc gccatccccg
     3421 gctgcccgcc cacaccctgc ggcgcggcgc cggccgcgcc cgtgtacacg acgacctccg
     3481 cctcgaccac gaagtccgag cgcagcttgc gatccacgat gccctcgagg taccagtgca
     3541 tgtagctcct ggacgccgcg aacgtcggcg gcatcccctc cggcagcggg atcgacgcga
     3601 agatgggctt cgtcttcacc cgccgcggga tcgtgccgga ggcgtgctcg agctggttcc
     3661 tgccgcggat cgtcgggatc ggccaggcga tggagtgggt cgccaccggg cgcgtcttcc
     3721 ccgccgccga ggcgctcgcc ggaggcctcg tcagcagcct gcaccccaag gacgagctgc
     3781 tcggcgccgc ccatgcgctc gcgcgcgaga tcgccgacaa caccgcgccc gtgtccgtgg
     3841 cgctcggccg ccggctgctc tggacgatgc tcggcgccga gcacccgatg gaggcgcacc
     3901 gcgccgactc gcgggcgatg ttcgctcgcg ggcagtcggc cgacgcgagc gagggggtca
     3961 ccgcgttcct cgagaagcgg ccggccgagt tcccggaccg tgtcagcgac gggctgccgg
     4021 agatcttccc cggccgcgag gtacccgcgt tccgctagct aggggagctt ctgctcgaag
     4081 aagaactcgt cgccgctcgg caggcggtcg tggcgcgtga acgttccgcg tgcgttggtc
     4141 acgcccctgg tgatcgcggc ccactggtag gtcttcgggt ttccgatcgc gcgcgggctg
     4201 aaggtgtacc tcgtcgtgtg ctcgctcgtg cgcaccaccc gcgccgagcc gagccgctcg
     4261 gcggtgccgc agaggtacac gcccaaacga ccgtggtagc ggccgacgaa gaaggtgcac
     4321 tcatccatgg cgcccgcgtc ggtctgtgcc tcgatgtaga tgtaggtggc gaggtcgccg
     4381 gaggctgggt ccgcggcgga gccggcgacc gtgaccgtgt gcttgatctg gccgtcgctc
     4441 gtgcggccgg ccgtggcgcg gacgatgtcg gcccggtcgg cgcctccgga gggagagccc
     4501 tccacgtcgc cgcgctcgtc ggtcccgccg ctggtcgcgg cgatgccgag cggcaccgcg
     4561 acgagaaccg ccgcgagggc gatcatcagc gctgcgagtc ccctcctcat tgcttaattg
     4621 ctacccacca gcgcggcccg aaacgtgaca catgtctcag agcgggccgg ttggcgccgc
     4681 cgcgcggctt gctatgcggg aaattgctcc agcgcccagg ccgggccgat ctcctcgcgg
     4741 cggtacgagc gcccggcgcg gcggacgtaa tcccccgttc gggggtcgag cggcagcccg
     4801 gactccatcg ggtaccactc gttgtgctcg cggatcagag cgttcaggtg gtcgaacggc
     4861 cacgcgtcca accgcgcgcg ccactggcgc ccgaacgcct cgcggtcctc gccgcaggcc
     4921 tccttcagcg cgaggtaggc gcgctcgagg cgaccgcgct ggatcttcag gctcgagtcg
     4981 atctccgcga ggcgctccat gtagcgcggc atgacgcccg cggcgaggta cgcctcgacg
     5041 gtgcgcttgg tctggcgcgt gcgccgcgac agcggcttgc cgtgcccggt gtcgtcctcg
     5101 aggctcctct ggatccgccg ctcgatcggc gtccgcacgt cctgttccat cttctgaaat
     5161 ctagggtgtg aggtccctgg cgccctgccg cttgaagacc ggccagcgtc gcaggagccg
     5221 tccctcgcgc acagcgacga gctcgtgata gccgaggatc gccccctcgg tctgggtggg
     5281 ccggtagagg acgtagtcgc cgagcgccac ggagcccttc gggacgatca gggcgtcgcc
     5341 cccgcccgag gcctcgtcct cgcggccgcc gggcgggtgc gcgagctcac tcgccgccgc
     5401 cactccctgg acgacgatct ccatctcggc ggcgccgggc tgcgcgacgc cgatcaccgg
     5461 cacgctgggg tggtcgctcg tgatccgccg caccgcgccg ccgagcgtga gcgccggcgc
     5521 caggccgtcg gtgtcgtagc cgccgttcag gtaggcggcg tacaggaacg ccgatccgca
     5581 gccgatctcg ttcgccggcc cgccggtcca gttgcggtag ttcgagctgc cgggaccgtt
     5641 gcggacctcg ggctcgtggc cgagcgcctt cagctcggcg agccgcgtcc ggtagacctc
     5701 ctgcgcctgg gtcgccacca ggcggcggta ctccgggtcg ccgcgcaggg tggcgtggcc
     5761 gtcgtagccg agcacggccg tgagcttcag ctcgcgcagc gcctccaggc acgccctcat
     5821 ctcatccgcg tcgttgatcc ctccgcggcc cataccgacg tcaaattcca gggcgacctc
     5881 gggcaccggc cggcgtgcgc tccgcgccag cgtcgccagg tgctcggcaa cggcaactga
     5941 gtcaatcagg atacgcagtc ggtgggggcg ctgtcccctt ggttgcttcg tgcgcaggaa
     6001 cgtctgcagt tctccgaagg tcggcgggta gccggtcatc aggtccgtgt tcctcggggc
     6061 ctgggcgatg atcgggtcca cctcgctcag gttgaagacg agcccgcgcg gctcgggcag
     6121 gcggttcagg aggtaggaga tcagcgccgg gctgcggaac gacttgagcg ccggccgcac
     6181 gccccagccc tgcgccttcg cgaagtcgac caccgtcctc gtgttctggt cgagcgccgc
     6241 gaggtcgagg aacaggcccg gctggccacg gccgtggtcg cgcgcgagct tggcgagcgc
     6301 atcgagcggc atgggcgcac cacccgcgcc gagcggcggc ttcgccggct ttctcttcgc
     6361 gagcgcgacc tgcgggttcg cggccgttac ggcggtcgcg gccatgaacg tccgcctgtc
     6421 gactcccccc atcgtgtttc gtattcaatc gcttacggcg agcagtgaga gaaggtggct
     6481 gcggagcgcc gtggcctgct gcatgcgctg gctcgtgacc cggatcggcc gccagcgggc
     6541 cacctccagc tcggcgctcc tgacccggtc gcgctcgaac gccgcgcggg tgccgtggta
     6601 ctcgtagctg tccagctcca ccaccacgcc gcggtcgagc cacgccatgt ccacctcctg
     6661 gccctccacc cacacgttca cgctcggctc ggggatgccg ttcgcgcggc agaactcgag
     6721 aaactcgatc tccagatccg agcgcgtctt gggcgagtcg ggccggtatt cggcgagcgc
     6781 cacgcgggcg ttcccgatcc ccctccgtcc cctcccccgc tcgcacgcct tgcgcacggc
     6841 gctcgcgtcc agcagcccgc gcctatcggc ctcctcgaat gtccgtcgca actgcttctc
     6901 ggagagggag gcaccgagat ccgtgagcgt gcgcgccacg ctcgtgacgg ggatgccctc
     6961 cacgacggtc acgtcctcgg tgtggagggt gcggacggcg tggagccggg tccccgcccg
     7021 cgcccggcgt tccttcccga ccacggtcac atggaccacc gagccgcggc tcctacgcag
     7081 gtcgtgcacc cacgccgctg ccagatgact cgccacggca tcgggcccgt aggcgtagac
     7141 cgcggccagg accctgcccc ggggcgcgag cgagcggtgc ccgagcgcgt acaccccgcg
     7201 gtagaggcgg tgcagccgcc gcgcagcaac caaccgcttc acgctgtcgg gtgtgaaacc
     7261 gagctcggag agctgccaga tcgcaaggat cccgtactgc cgcgaggccc gtgcagccag
     7321 cgcggccagc ctctcctcac gaggtgtccg gattttgtgg cgcataccgc cactaagtcc
     7381 gggcacctcg tatttcgccc ccgtgggggc tccgcctact ccttgcctgg cacgcggcgc
     7441 agggcgcgta ggcccagcgt tatcgccttg gtccacacgc gctggcccac gcgcgggggc
     7501 ggggcgatcg gcagcaggcg ctgcaccgag acggtctggg actccgtgta cttgaggatc
     7561 ccgtcggcgc cgtggcggcg gcccatcccg gagtccttca tgccgcccat cggcgcgtcg
     7621 accgagcccc aggtggcgat gtaggcctcg ttgatgttca ccgtgccggc ctgcaactgg
     7681 acagccaggt gccgagcacg atccacgtca gacgaccaga cgctgaagtt gagcccgtag
     7741 tcgctgtcgt tcgcccgcgc cacgacgtcc tcctcggacg agaacgtgga gatcgaggca
     7801 acgggtccga acgtctcgtc gcggaacagc gtcatgccgt cgcgcacgtc ggcgagcagc
     7861 gtcggctcgt ggaagtacgg gccgatgtcg ggccgcgcgt tcccgccggt cagcacgcgc
     7921 gcgcccttgg cgacggcatc ctcgagatgc tcgcgcaccg tctcgagctg cttcgccgag
     7981 atcagcgatc ccatgtcgca cgagtagtcg agggagttgc cgagcttcag cttctccacg
     8041 cgctcgacca gccggcgcgt gaactcgtcg gcaatcgact cgtgcacgta cagccgctca
     8101 accgagatgc agagctggcc cgcgttcgag aacagcgcgc gctcggcgcc ctccacggtg
     8161 cgggtcaggt cggcgtcttc gagcacgatc atcgcgttct tgccgccgag ctccatcgag
     8221 ctggggacca gacgctcggc cgcctgcgcc gcgacctgtc gacccaccgc ggtcgacccg
     8281 gtgaacatga cgtagtccac acgcgagatc agctccggac caagctgcgc cccacttccg
     8341 gtcactattt gaagcagttc agaaggcagc cccgcctctt ccaaaagcgc gagcccgatc
     8401 aacgcgctga acggcgtctg cgtgtccacc ttcatcacca ccgcgttgcc cgcggccagc
     8461 gccggcacgg catcgctgat cgacagcgtg agcgggtagt tccacggcgc gatcacgccg
     8521 accacgccga ccgggtggtg gtactcccac gtcgccgtga gcaccggcag cgcgccctga
     8581 cggcgctgct cgcgcaggtg acgctcggcg ctgcgggcgt agtagcgcgc gaccacggcg
     8641 gcgtcgagga tctcctcgta cgcgtgccgg cgcgccttgc cggactcgag ctggatcacg
     8701 tcgaggatct cgtcctgacg gtcgaggacg aggtcgtgga agcgcagcag gatctcccgg
     8761 cgctcctcga atgagctctc gcgccagcgc gcctgggcga cccgcgcgcg ggcgaccgcg
     8821 gcgtcgatgt cctcggcggt gcagcgcggc acggtggcga gcaccgaccc ggtcgagggg
     8881 ttctcgatct cgatggtctc ccgctccccc tccccggcag agacgcgagc cgcgagcgcg
     8941 gcgagatgcg caccggtcac gcgcttcgac ccggtgtcga gaggcgccga tcgggtgggc
     9001 tccagggtgg tggtcatcag cgcctcctca cggcgtagcg gatcgagtag aagtccaacg
     9061 agtgcgggag cggctccttc gagccggcgg gaagctcggg ctccagcacc gtcacgtccc
     9121 aggccgcgag catctgcgcg agcacggcct tccccagcag cagcacgagc ggctcgccgg
     9181 ggcaccactg ggatccgttg ctcaggtggt tgaagcggta gtcgcgctgc gaggactccc
     9241 agcgctccgg cttgagcagg tcggcgtcgg ccaccccgtc ggggtcgcgg tggttgaacg
     9301 cgttgaccat cagcacctgc gtgccctcgt cgatccgctc gccggcgagc gtcgtgtcgc
     9361 gcgtggcctc gcgcgcgatc agcggcgtgg tcggccacag ccgcatcgtc tcctggaggc
     9421 agccctcgag gtagcgcagg ccgtccacgt ccgcggcgct cgtgaggtcg cggccgtcca
     9481 tctcctcgcg cacgcgctcc tcgaccgcct cgtccgctgc gatcacggcc agcgcgcggt
     9541 acgcgttcgc ggcgagcgtg tcgcgcatcg cgaagaccca gtgcgggatc tggtgcacca
     9601 cgcgcgtgcg gtcggtctgc ggcgcgtccg cgaagcgcgc caccagcgcg ccgggctccg
     9661 ggtcgcgcag gtaccactcc atccgcccgt gcagctcgtg gtaggcgtcc gaggactcgg
     9721 cctcgcgggc cacgatccgg ttcgcctcgc acatcagctc gctcagcaga gcggtcaggt
     9781 cctgatcgcc gcgcgccttc tcgccgaaga tcacgcgcag cgtgacgtgg tcgaacagcc
     9841 gctcccagcg ctcccattcg acccggccgc tgatccgcat gcgcgacatc tcgtcggcca
     9901 ccacggacag aaagcggtcg ccgagcgggt gcacccgctc ggcggtggcg agcacggact
     9961 cggtgaacgc acgccggtcg cgccactcct ctccgcgcga gatcgtcagg gcgtccggct
    10021 ggaagtggca catgcccttc tgcttggcgc ccgcgtcggc ggcgtacacg tcggcggact
    10081 ggtcgagcac ctcgcggatc gcgtcggcgc cccacagcac caccatccgc ccgcccagca
    10141 gccgcgcgcc ctggcccggg tgcttgcgct tgatcgcggc gagcgtctcg acggtgcggc
    10201 gatccgcgtc gagcgcggtg agccgcttca tcgctcgcct gcgcggcttg aagagcccgc
    10261 gcgtgatggc ggggaccacg ccggctacaa ggaccttcgt gctgtcggcg acggaggccg
    10321 aggggagcgc ggcgggcgga gcctccgtgg tggtggtcac cggttgatcg cctccatcgc
    10381 cttctggatg ccctcgacgc gcttcttcgc gctggtcacg cgcccgtcgc cctcgctgcc
    10441 gcccttgaac tgctcgacgc ggtgccacgg caggaaagcg ttcggcatcc cctcgagcgc
    10501 cggctcgagc tcgggatagt ggcgcaggat cacgtccttc atcttcgtcc tgttgaccca
    10561 cctgatcccc agctcggtgt agacctccgg ccggaagtcc gtggtgaaga agcgatcgct
    10621 cttcagccgc cgcgaggcca tcagcacgaa cacccggaac gccgtgtcgg agaacccgaa
    10681 gcccggcggc agcggctcgg cgtacatgcc gatctggagg tcgaccttgt cgatgtcgtt
    10741 gtcgtagacc cgccggatct gctcggccca caccttgttc ggggtgagct cgtcgaacga
    10801 gcgcacgcgc ggcatccgca tctcctcgcg gaagtcgttg tagcgcggca cgccgcgctc
    10861 gcggtcgcgc aggatgtcga gcgtggcgag gtccatcacc tcgccgtcga tccgcacgtg
    10921 gcgctggagc gcgcgcgggt agttgtggag cgtgatcgcg cccgggtgct cgacgccgag
    10981 cgagtagaaa aggtcggtca gctcgtaccc gtccatgaac gagcgcgtgt tcttggtggc
    11041 gagctcggtg aactcgcgct cctcgcgcac ggacccgctc ccgaagtcgt agatcttcca
    11101 gtcgtcgggg atcagcgggt gcatccggta gaccgagacg aactcctcgg tgagctggaa
    11161 gcgcgcctcg tggtgctcgg cgggcgagcc catgatcccg ctgaagatct ccgagtcgcc
    11221 gagccggccg atcaggtcct tcgtgtgctg gccgagcacc cccgaccagt tcgcgttcat
    11281 cgccgcccag agcgccgtgt tgttcacgat ccccggcgtc cactcgaccg tgtggatctt
    11341 cgccatcagc gcggagttga cgagccacgc ggtgtcgaag agccgctggt cgctccagcg
    11401 cgggttctcc ttcttgagcg cgtcgcagat cgcgttgtgc tccttcgtga acagcgtgtg
    11461 caggcacgag agcccgaacc accagttctc gttgaagccc gtgaggtcca cgccgtcctc
    11521 gtcgggatcc ggcagcatcc ggccgtcgtc gccgatcttc agcttgccgt cctggaacgt
    11581 gcgcagctgg ttctgcttct cgatgcccac gccgtagagc tgcgagccgt cccaccagtg
    11641 cgtctcggtg ttgccgaagt tcacggagcc gtcgccgttg tcctcgccgg acgggacgct
    11701 gacggtgcgg cgcacgcgca tcgggtcctc gtgccagtcg tcgcccggct cgagcgggac
    11761 gtccatcgtg ttgtcgggct cgccgcggcc gtggaagaac cagttgtggt tctcgaactg
    11821 gatccacgcg gcggcgagga tgttgagcgt cgtggcgggc ttgaagctgt cgcgcgtcat
    11881 cagctcgagc gagaccgtgc gcgcgctcgg gtcgtggatc cgaggcggct tctcgggctt
    11941 gatgcggcgc gtgtcgatgt tgcggccgaa cgcggtgccg gtggagccca tgtcgggctg
    12001 cacgaggtcg ttccacttgc cctcgggatg gcgcgcggcg cggtactcct cgggcggctc
    12061 ctgctcgggg cccgcctcgt agacgggagt cgtgtcgtgc aggttcatgt cacgcaggtc
    12121 gagccgcagc gacagcaggt tcagcaggtt gatcgggaac ggcagctcgt gccactgctt
    12181 gcgttcgttg atccggccga acacgccatg cagcgcgcga ccccccagcg acctcttcat
    12241 cgacgcctac ctccctgtcg gggaccgtcc tgagacggcc ataccctgac acagatcgat
    12301 gtagtcgtcg gcgcgcctca gcgtgtgggc gctagcgcca gggtgcgctt gcgggtcgcg
    12361 gagttgcccg cggggtcgag cgcccggagc gcgatcctgg cccgcgcgcg ggctcgtaac
    12421 ctcggctgct tcagcgctcg gcgtgccttc tcggggagcg ccagccggac cgtcgcggtc
    12481 ccgcccgccg gcaccgcccg cgtcgcgccg gtgagccgga acgcccggcc cgggagccgc
    12541 agcgaggccg acgcgatggc cgtgcacggc tcggcgccac agcccacggt catgagcagg
    12601 cggccgccgc gcagggtggc ggcaacgatc cgcgggtcgg gcggcgtgag gtccgcatcc
    12661 gcggcggcgg atgtcgtcgg aggcggcggg acggcatgct gcgcggccgc atcgctgggg
    12721 ggcgggtccg cgcctgggtt taccgcggcg cccgcaggtg ggcccgcggc cggcgggggc
    12781 ggcggcgccg gagagcacgc tgtggcgacg tcgtagccgg agtccggcgg gcgtcccgcg
    12841 atcgcgttcg cgtactgacc caggctcggc ttcgggggtt cggagaggga ggcgggattg
    12901 agcccggcgt ggaacggcca ggccgtcctg gtcgtcgcga cgtcgcgcca ctggaactgg
    12961 atggccgcgc tgagccgcag ctcgtcgcga agcgcccgcc acgcgccggc ggcggcgtgc
    13021 acgcgcgcgg cctggcaact gggggtggtg acgaacggcg agttcgcacc gccgctcgcc
    13081 cagccccact cggtgatcca gatcggagtg gcggtgtctc cgtgcgcgtc cgcgatctcc
    13141 cggaccatcc tcggaatctc cacgaccccc tcgacgtcct ggcggtaggc gtggatcgcg
    13201 agcgcgtcga agtacgccgc cgcgcccgcg tcgtacagcc cgttgaggta cacgcggaag
    13261 tcctgccggc tcggcgtggt gaagctcggc gcgaggccgg ccatcaccac ctccgctccc
    13321 gggtcggcca gcctgaggtt caactgggcg tgcttcagga gctccacgta ctcggccggg
    13381 tcaggctcgg aatcgccctt ccaccacgcc gggaggttcg gctcgttcca gacctgccac
    13441 gcgcggatgg gcagcacggc gctccggcag gtccgcggca ggcccgagca ccagaacgag
    13501 ccgttcggcc cgtagcgctc ggccagcttg cgcaggaagc ggcccaggta gacggggtcc
    13561 gcgggcggga cctggcggtc ggtccgcgcc ccatccggct tgtagaactc gggcgtgctg
    13621 aagaccaccg gcaggacgcg cagccccctg tcggccgcgt ccccgatgat ctgatcgagc
    13681 gcccgccagt cgtagacgcc ggtcgcactt gcgttggtcc actcggtctc gacctggctc
    13741 cagaagacgt ggtggcggat cgtcccgatc ccggccgcgg cctggcgatc catgtcctgc
    13801 tggcgctgct cgcgtgtgag ggcggcgttg tcgtcgaaca gcgtcccgaa gaacgggtcc
    13861 ggcgcggcgg ccgcgtgggc gggaacgcac atcgcgagca cgacgatcag cagcgttagg
    13921 ccccggcaaa tcacccgcgg ggcatccccc gaatcgggga ttcccgaacc ttaagtggag
    13981 gcggccggtt ctgccccggc gtccgcggaa cccaaccccg atgcttgcta cgagtgttcc
    14041 cgtcggagtc tgatctcggc caccgggtcg gtcgatccgc gggacacgtc accggcgcca
    14101 caccctcctg ttgatgtccc caccgggcgg atggtcagtt ccggtgggtg agcccgctgg
    14161 ttgaagccgc ttgaccgggt cacgggctac ccggggcgac tgcccttacc ggtgtttagg
    14221 cggcaagggc gaactcgtga gagtccgcac ttattggttc ccggatcatt taacgaggcc
    14281 aaccggggtc ctcgactcgc cacatcgagg cagacagctc cacgtcgaag ctagttcgcc
    14341 ccctatgtcc gctcaatggt agtcgcgagc ggtcgctgcg gcaccggcga ggactgcatg
    14401 atcgagctcg tggtctgccc gagggcggcg aagcggtcga tcacctcctc gaggtgctcc
    14461 acgtcgcgca ggtgcgcctt catcacgtag cagtcgtcgc cggtcacgcg gtggcactcg
    14521 acgacctcgg cggtcttgcg ggccacgtcc gccacggtct tcagccggtc cggcgccggg
    14581 cgcacccgga tgatcgccga gagcgagtag ccgaccgcgc gcgggtccac cagcgcgccg
    14641 tacccgagga tcgtgccgtc gtcctcgagg cgctgcaccc gctcggccac ggccggcggc
    14701 gagagcccga ccctccgccc cagctcggcg aacgtgacgc gcccctcgcg ctggagctcg
    14761 tcgagaatga ggcgatccgt cgaatcgctg ccgttacggg ccgaagccct ggctttcaaa
    14821 ggcacagctc ggatactacc tgtggataga gggtgcaaac gtgcaatagc cgacgatgct
    14881 tccgggcacc atgcgccgcg agcccccgcc ccaggtctac ttcgtcttca gcgcggtctt
    14941 ccactacctc ggccccgcct tcgccgtgct cctgttcgcg cgcgtgcagc cgctcggggt
    15001 ggcgtggctg aggatcgcct cggcggcggc gatactcggc ctctggcgcc ggcgcatcgt
    15061 gctcagtcgc ctcgtggtgg cgtggggagc ggtgctggcg ctgatgaact gctgcttcta
    15121 cgtggcgatc gaccggctgc cgctcgggac ggtcgccgcg atcgagttcc tgcccgtcat
    15181 cgtgctggcg gcggcgggcg cgcgcacggt gcgcaacggg gcggcgctcg cgctggccgt
    15241 gcccggcgtc tacgtgctgg ccgacgtgcg gctcgagggc gagccactgg gcgtggcgct
    15301 cgcgttcgcc aacgcgctcc tcttcgcgct ctacatcgtg ctggcgcacc gcgtggcgca
    15361 gcacgcgtcg atcggcggca tcgacggcct cgccgcgtcg atgctcattg ccgcgatcgt
    15421 ggtcacgccc atggccggct gggccgccgt gccggcgctc acggacccgg tggcgctggc
    15481 ggcgggcgtc ggcgtgggcg tctcgtcgtc ggtcatcccc tacatcgccg accagctcgc
    15541 gatggcccgc ctcacacgtg ccgcctacgc gctgatggtg tcgctgctgc cggcgaccgc
    15601 gacggtgatc ggcgtgatcg tgctcgcgca gatcccgtcg gcgcgggagg ccgccggtgt
    15661 cgcgctggtg gtcgccggcg tggcgctcca ccgggaggca cgcccggcgc ggaacgagag
    15721 cgctcgcggg cagcatcgcg cagagcacga gcagcccgcg agcgcgtgac tacttgtagg
    15781 tctcagcgcg gctagcgcac gaagtcgcgc acctgcccga acacggtggc gtcgccgtac
    15841 agcccgctgt gcgagaggca cgcggtctgc gtgttcgtgg ccccgccgag cgccacgctc
    15901 gaatccgggt tgatgacctc gtcgcacggc gaccaccagg tcccgaagcg cggggtgccg
    15961 ggggtctcgt cgcccgagtt cagcgcggtc aggaacgagg agccgatccg catctcgccg
    16021 caggaggtgt cgaagcagaa gttcgcggtg tcggtcccgt ggttgggccc gccgagcgag
    16081 acccactcgt cgaccttcag atcgccgccc aggttcttga cgtagtagcg cgacgagagc
    16141 ccgcccatcg agtggctgat gatgtccacc ttggtcgcgc cggaggcggc caggatcgcg
    16201 tcgaccttgt cgcggatctg cgcggccgtg gtcgcgttcg actgcgcggt gttgtagctc
    16261 cagttgttga gcgacgagga tggccagccc gcgctctgga agcggctgat catgtccgtc
    16321 cagacggtcc cgctcgagtt ccacccgtga acgaagagga tcgggtcgta cggcgtcgtc
    16381 gtcgtcccgc cgcccccgcc gggcttcgcc gcaagggcgg accccgccat caccagccac
    16441 atcgcagtcg tcgcgcacgc gagcgcgagc ccccgtccct tcatgcaatc cctcccgttg
    16501 ttgaatgccc ctaagaaact gcggctggga agtacccccg ccccatgcgg gccaaccccc
    16561 cgacgaatgg gggttcttag gcgggttcga cggcgaagac ctcgaggccc ttgggggcac
    16621 ccttcgcctt gaagcggcgc tggcgcttgc acttgaacgc accgtcctcg aggcgatcgc
    16681 aggtcggtcc ggagatgagg atctggtccc cgctggcggc gtccgccacg cgggccgcga
    16741 tgttcacgtc gacgcccagg tagtcgccgc cgatgcggcg cgggcggccg aggtgcacgc
    16801 cggcgcgcag ccgcggccgg tagccgtcct gctcgatcgc cgcgacccgc tcgcgcacgt
    16861 cgcacgcggc aagcaccgcc tcctgcgggt cgttgaagac ggccatgagc ccgtcgccga
    16921 gccgcttgac cacgcgcccg ccgtgctcgg ccaccgccga gtcctcggcg cggccgacct
    16981 cgcgcagcag cgcaagcgac gcctcgtcgc cggcctcgag cgcccaggtc gagtactcga
    17041 ccaggtcggt gaagaggatc gccagctcgc ggtcaccgcg cccgcggccc tgcttctcgg
    17101 agatcgcctg ccagacctgg aacgcgccga gccccagctc gcgcagggcg ctcggccgct
    17161 gctgacccac ctcggcgacg cgccgggcga gcacgagcga ggggtcgtcg cccgccatcg
    17221 agagcgggtc ccccagctcg ctgtcgcccg gcagcaggcg ccggacggcg cgggccgcgt
    17281 tgagcacgcg cggcgagccg tccgcgaggc gcagcttcgc gagcgcggcc agccggccgc
    17341 cctccgcgtc cgctgcctcg agctcggcct cgacctccgc cagctcgccg tcggtgccgg
    17401 cctcgacctc cgcggcctcg ccttcgacct ccgcggcctc gtcttcacgt ggctcgtcgc
    17461 tcacgccgtc aagggtacgg ccgctacgcg ggcacgaacg cctcgccccg aaagccgtcc
    17521 gggtcgagcc ggaagcgcag ctcgggcgcc cccgccgtct ccatcgacgc gcgcacccgc
    17581 tcggggtcgg ccgcgtacgc gagcaggtag ccgccgcccc ccgcgcccat ctgcatgact
    17641 ccctcggcac cggcgccgag cgcggcgcgc cgcagctcgt ccatccgcgc cgtggccatg
    17701 ccgggcgcac gggcgagctt cgcgtcccac tgctcgttca tcagcgcgcc gaaggcgccg
    17761 aggtcgcctc gctcgagcgc gtcgcgcacc gcgtgcgcga tctcgacggc gcgcccgagg
    17821 ctccgctcga cggtgccgtc gccggcctgg gccgcctgcg cctcgccggc cagcaggtcc
    17881 gaggccgagc gctgctcgcc cgagtagaac agcagcagcc cgtcgtcgag cgcgccgatc
    17941 accgcctcgt cgagcgccag cggctcggcg gccacgacgc cgtcgcgcgc gatcccgtac
    18001 gcgcggatcc cgccgaacgc cgaggcgtac tggtcctgct tgccgacggt ccgcctcagc
    18061 acgccgatct cgatgtcgca tgcctcctcg gccagctccg ccgcggcggc cgggctcccg
    18121 cccgcgagcg cgagcgcccc gagcgcgcag accgcgtagg cgccggacga gccgaggccg
    18181 gtccccggcg gcacgtcgga ggtcgaggcc agctcgagcg gggcgccgtt ccagcgccgc
    18241 agcagcgcct cgcgcaggat cgggtgctgc acctgctccg gcgcgtcggc ctcctcccac
    18301 tccatgtgct tgagccggat gcggtcctgg tgctcggtgt tgacgagcat gaagacgtgg
    18361 cggtcgatgg ccgcggccac cacgtagccg ccgttgcggc cgtaccagga gggcaggtcg
    18421 gtgccgcccc cggcgagcgt cacgcgcagg ggcgagcggg cgaatacgac gggcacggcg
    18481 gcactgtgac agttcgggcg cgagggtggc cgggtaaacg gggggcgtgg acgacctctt
    18541 gcggccgagc ctccaggagc acgaaccggc ccaggcggac gcggccaagc cgtggcgcgt
    18601 cagctcccag gtctacgtcg gcttcttcgg cggagcgctg gccgtcggcc tgatcgcgtt
    18661 tctcaacacc taccggctgc gttgccccga ccgcagccgc ctgctggtgg tcggggcgac
    18721 gctggccggc tgcgccgcca ccgccacgat gctcgtgctc gggatcgagc gcccgagctt
    18781 tcaggtcggc ggcgtgaccg cctacgggct cgtctacctg atccagcgac gggccgaccg
    18841 cgccttcgag atcttcgacg gcgaccacga gtcgctcgtg cgccccggca tcgcggccgc
    18901 cctcgccggc tggttcctca gcctcaatgt catcgtgtgg gcctcgtgat gcggaccgat
    18961 gccgacaact ccgcactccg gatggccgag tactacctcg agatcaaccg gccggaggag
    19021 gcgctgcacg ccctagacag ctcgggcgtc gcgctcgagg agccgggcgc ctggctgatc
    19081 cgcggcacgg cgctgttcgc cctggagcgc cacgacgagg cgggcaaggc cgccgaggag
    19141 gggctgcgcg aggatcccga cgacgtctgg ctgctgcacc tgctgtcgat cgcgaaggag
    19201 gcgctcggtg acctcgccgc ctccgagcgg gcgatcctcg ccgcgctcga gctcgagccg
    19261 gacatcccgg ggctctacac gcagtacgcg gacctgctga tgcgtgcggg ccagctcgac
    19321 aaggcgcacg cggtgatcga gcgcgcggca gctctcgacc ccgaggacct cagcgtgctg
    19381 cgcgcgcgca tgtcgtacgc gtacgtgacc gggaaggcgc gcgagggcga gaagtggagc
    19441 cgcaagatgc tcgagctgga gcccgagcac cccggcgccc acggcatgct cggcgtgttc
    19501 aagatcgagc aggggtcggt catcggggcg cgcgagcact acgagaccgc ggtggcgctc
    19561 gatccgtcca gccccgacgc gcgcgaggcc acgatcgaga tccgcaccgc aacgcacccc
    19621 gcgatgctcc cggcacgcgc gctcgaccgg ctcggcgtgg cgggatcgtg ggtcgcggcg
    19681 gtgatcgtca tcaccgcgct gcgctcggtc tcgccggggg ccggcgcggt cgcgggcatc
    19741 acctgggtga cgttctgcgt ctacacgtgg gtcgcgccgc cgatcgtgcg ccgcctcgtg
    19801 attcggaagc tctcgtgacc gaccggatcg agggactccg ggccgcgctc gaggcgtcgc
    19861 cggacaacgc ggccctgcgc gccgtgctcg ccgagaccct cgcggacgcg gggcaggtcg
    19921 atgaggcgct cgaccactac gaccggctgc tcgaggcgcg cgcgctcagc ggcgagcagg
    19981 cgctcgtggc cgggcggctg gcgctggcgg ccgagcgcta ccagatcgcg gcgcgctgcc
    20041 tcgagcacgc caagtcgcag ggcgcgctcg agggcgtggc ggacctccag cacgacctcg
    20101 agcgcgggct ctcggcgggc ggggatccgc ccccgcgcat cgaacgctcc cccgccaccc
    20161 caccggagct ccagcccgac gagctgctgg acgaggagcg gcggatcacg ttcgcggacg
    20221 tgggcggcct cgcggaggtc aagaagacca tccaccgcac gatcatcctc ccgttccagc
    20281 ggcccgacct gtatctcaag tacgggcgcc gcgcgggcgg cggcgtgatg atgttcggcg
    20341 cgcccggctg cggcaagacg atgctcgcgc gggcgaccgc cggcgagtgc gggctgccgt
    20401 tcttcaacgt acggatcgag gacatcctcg acccgcacct gggcggctcg gaggagcagc
    20461 tcagcttcgc gttcgagacc gcgcgcgcga gcgcaccgtg cgtgatcttc ctcgacgagc
    20521 tcgacgcgct cgcctacgcg cggcgcaagc agcagggctt cgggcgctcg ctcgtgaacg
    20581 cgatcctcca gcagctcgac gcgatcggct ccgataaccg cgacctgctg atcatgggcg
    20641 cgaccaacgc gccgtgggac gtcgacgacg gcctcaagcg gcccggccgc ctcgaccgcg
    20701 tgctgttcgt cccgccgccc gacgaggagg ctcgcgcgcg catcctcgag ctggggatgc
    20761 gcgaccggcc gacggagggg atcgacatcc gggcgctcgt caaggccacg ccgctattca
    20821 gcggggccga cctgcgggcg ctggtggagc aggccaccga cgaggtgatc gacgaggcgc
    20881 tcgactcggg cggcgagccg ccgatgcgga tggcccacct cgaaaaggtg ctgcgcgaga
    20941 tgcgccccac cacgctcgac tggctggcga gcgcgcgcaa ctacgtccag ttcgcgaacc
    21001 agtcgggccg ctacgacgag gtggccgcgt tcctgcgctc gcgcgacgcg aagaaggcgt
    21061 tgaaggagat cgcgaagcac gacgagtagg gggcgccggc gcgaccggca ggcgcgggtg
    21121 gcgccgcggc ccggagggcc gggcgcggcc ggcgggcggc tagcgccggc gcgtccccat
    21181 ctcccgctcg atctcgcgct tcatgtcctt ctccttgagc gagcgacgct tgtcgtagag
    21241 gtccttgccg ctggccagcg cgatctcgac cttcgcgtgc ttgcccttga agtacatgcg
    21301 cgtgggcacg agcgtgaggc cgcgctcgga gaccttgccc accagccgct cgatctcgcg
    21361 ccggtgcagc agcagcttgc gcgggcgctc cgggtcgtgg ttgtcgcggg cggggtcgta
    21421 cggcgcgatg tgcacgttct gcagccagac ctcgccctcc tcgaccgccg cgtagccgtc
    21481 cttcagctgc acgcgcccgg cgcgcaccga cttcacctcg gtgcccatga gctggatccc
    21541 gcactcccac ttgtcgagca ggttgtagcg gtgcgaggcg ttgcggttac gggctatgtc
    21601 gcgctgcggg ggcatggcgg cgggcctagt gtgccgcgtc cgcggccagc tcgaggtcgg
    21661 cgcgtccgcg cggcgcgtcc acgcggccga ccgccacgcg gagcggatcg cccagccgga
    21721 tgcggcgtcc ggagtcggcg gcgaccagcg cggtctcctg ctcgttgatc tcccaccagt
    21781 caccgcgcat ccggcgcacc ggcaggaagc cctcgaagcc ctcgtcgccg aacttgacga
    21841 acaggccctt cgagatcact ccgaccacct cgccgtcgag ctcgaggtcc cagcccgcct
    21901 cggccaggcg gcgctcgagc aggaaggcca tgcagacgtc gtcggcgccg cgctcgatct
    21961 tcatggcgtc gcgctcggca gtcgagctgt gctcggcggc gtcgaacagc tcgtcggcgc
    22021 ggggaggcgt gtcgtccgcg ccgatcgtcg cgagcagcgc gcggtgggcc acgaggtcgg
    22081 ggtagcggcg gatcggcgag gtgaagtggc agtagcgctc gctcgccagc ccggcgtggc
    22141 cgatgttgcg cggcgagtag tacgcctgct tcatggctcg cagcacgagc gtgccgaacg
    22201 agcgcttgcc gcggccggac ttccggacgt agcgcgcgat gacgtggctg atctcggcgg
    22261 cggcgtcgcc cgcctgctgg gggctcatgc cctcgggcag cggcggggtg ggcacgtcga
    22321 gcgactcgag ctgctccgcg aggcgttcga cgccgcgcgg gtccggcttc tcgtgcacgc
    22381 ggtagagcgt gggcacacgc cggtcggcga gctggccggc cacctgctcg ttggcgagga
    22441 tcatcagctc ctcgatcagc ttgtgcgact cggtctgctc ctcgtgaccg acgccgacca
    22501 catggccacg atcgtcgaac tcgaacacgg gctcggccga ctcgacggcc agggagccgc
    22561 gccgctcgcg cttctcgcgc agcgcggccg acacctcgcg cgcgagcgcc agcggctcgc
    22621 cccacggctc ctcggcgcgc tcggagccgg cgaagatccg gtccacctgg gggtaggtca
    22681 atcgcgcgtc gctgcggatg cgcgagcggt ggaacgcggc cgagcgcacc tcggcgccgt
    22741 ccatctccag ctcgaccgac accgcgagcc gatcctgacc gctcacgagg ctgcacgcgc
    22801 cgctcgacag cgcgtgcggc agcatcggct cgaccgcgcc agggacgtag acgctcgtcg
    22861 cgcgccggaa ggtctcgcgc tcgagcacgc tgccggggcg gacgaaggcg ctcacgtcgg
    22921 cgatgtggac ccagacgcgg atgatgccgc cgtcctcgcg cgtggccgac accgcgtcgt
    22981 cgtagtcgcg cgcggtggcc gggtcgatcg tgaaggttgc gaggccggtg aggtcggcgc
    23041 gcggggcgtc gttcgccggg gggtcctggg ccgcgtcctc ggcggcgccc tcgacggcgc
    23101 gcgggaacga gcgccgcagc ccgcgctcga tcatcagcgc ctcgagcacg tcgcgcgcca
    23161 cgtcggggcg cccgagccgg cgcaccacgc gcacgccgcg cttgccggcg ccgagcagca
    23221 cgaggtcgcc aacccccgcg cccttcgcgg agtcgagcga cacatggcgc ccgcgttcga
    23281 agacggggct ggcgaccatg aagcggccgc gcttctcgag gactgcgacg gtgggtgcga
    23341 gggaggcgcc gcggggcgtc aacgcgcggt gcgcgcgacg cgatcgacgg cgaccggcag
    23401 cgcctcgtcg cgccgcgtgc gcggcaggtc gcgcgccctc acctgcggcg ggatcccctt
    23461 ctgagcgagg ttctcgccct tcggcaggta gtagctgccc acggtgagct cgaggatgct
    23521 gtcgtcggac aggaactcga cctcctggaa cacgcccttt ccgaaggtct tctcgccgac
    23581 cacggtcgcg cgacccttgt cgcgcagcgc gccggtgacg atctcggagg cgctcgcgct
    23641 gccgcggtcg accagcaccg ccatcggcag gtccggggcg agggcggagc cctcggcgtc
    23701 gaacttgcgc tcggcgcggt tgcggccctt cgtcgagacg atcagcccgt cctccacgaa
    23761 caggctcgac acgagcacgg cctcggtgag cagcccgccg ccgttgccgc gcaggtcgaa
    23821 cacgatgccg tcggcgccct gcttgagcag cggctcgagc tccttgcgca ccgccgcgtg
    23881 cgcaccctcg ctgaacgcgg cgagccgcac gtacgcgatc ttgcgcccac cgctcttctc
    23941 catcctgccc ttggccagcg gcacgtcgat cttcgcgcgc tcgacgtcca cgtcgcgcgg
    24001 cttcttctcg ccggccggca ggaccgacag cttcaccttc gtgcccgcct tgcccttgat
    24061 acgtccggtc gcgacatcgc tggagacccc cgcgatcgac ttcccgttca caccgacgat
    24121 caggtccgac ttgtggatgc ccgcgcgcga ggccggcgtg tcctcgaaca ccgacacgac
    24181 ctcgaggccc ttgtcgttcg gctgcacggt cacgccgatc ccctcgaact cggcactcag
    24241 cgcctggcgg aattccttcg cctcgtcggg cgggtagtag gccgagaagc ggtcgccgag
    24301 cgccttcacg atgcccttca gctcctggcc ctcgagcttg tcggtgtcga ccttcttgta
    24361 gtagtcgtcc tggatcgact cgagcagctc ggcgcggacg ctgcgccggt cctcgacgaa
    24421 cgcgtcgcgc agcggatcgg gcagcttgcc ggggtggccg ccgagccaga ggccgccgac
    24481 gaacacgacg atgacggtcg cgcaggcgaa gaaagctgtg agggcgctac gcacccgtcg
    24541 agattagctg cgcgcctaaa cgcggaggaa acgccgcagc gtgatgccgc tgccgagcga
    24601 ggacaccagc acgcaggccg ccatcagcat cgccacgagc agcgggaagt cgatcgtgtc
    24661 gggcgcggcg agcagcgcga agcggtccga cagcgggtcc accagtgtct ccttcgcgat
    24721 cagcagcatc agcgccgcga tcaccccgcc catgaacccg acgacgatcc cctcgatcac
    24781 gaacggccag cggatgaacc agttggtggc acccacgagc ttcatcacct cgatctcgcg
    24841 ccggcgggcg aacagcgaca gccggatcgt gttcgcgacg agcgcaatcg aggcgaacac
    24901 gaacagggcg gccagcgacg cggtgagcgc cttcactagt ccggtggcgt cgaggatctt
    24961 gtcggtgtcg cgctcgcggt tgcggacctc gtccacggcg gcgagccgcg aggtgcgctc
    25021 cccgctcggg ccgcgcggcg cgatgcggtc gacgatcgcg ttcacgccct cgggatcgcg
    25081 cggcgagacg cggaacgcgt ccggcagcgg gttgctcccg agcagctcga aggcctgctt
    25141 gttgcgcttg gtctgctcct tcagcgcctc ctgcttggag acgaactcga ccgaccgcac
    25201 gttcggcgtg gcgagcagcg cctggcgcag ctgggtgcgc tgggcgtcgg tggagccctg
    25261 cttcaggaag acgtcgacca ccacgcgctc gcgaacgtcg ttggcggtgc cggtcgtggc
    25321 ctgcacgacc gggatgaaca cacccagcac gagcgcagtg agcacgatcg tgatgagcgc
    25381 cgcgaggctc ggagccgcgt tgcggctgag cgcgcggaac gcctccttga ggaaaaagcc
    25441 gacccgggtc acgggctaga ggtcctcgcc gtagagcgcg cggcggacct tctcctcggc
    25501 gtagcggtcg cggtcgccgt cggtgaagtg cctgtggcgg gcgtcgagcg gcacgtggtc
    25561 gggctcgagg tcgcgcttga cgcgggtcac ggtctccttg ccgtagccga gctcctcgcg
    25621 gacgcgcatg gcgaactccg ccgtggactc ctcggcgcgg tagaggccgg actcgtcgtc
    25681 gcgcaccacg cggccctcgc tcagctcgat cacgcggcgg cgcatcttgt ccaccatctc
    25741 gcggtcgtgg gtggcgacga ccacggtcgt gccggtccgg ttgatccggt agatcagctg
    25801 catgatcccg atcgaggtct cggggtcgag gttgccggtg ggctcgtcgg ccaggagcag
    25861 cggcgggtgg ttcacgaacg cgcgcgcgat cgagacgcgc tgctgctcgc cgcccgagag
    25921 ctggtccggg tagctgtgga gcttggtgct cagaccgacc aggcgcagga tctccggcac
    25981 cttcttgcgg atctgctggc ggttctcgcc gatcacctgg agcgagtacg cgacgttctg
    26041 gtacacggtc cggttcggga gcagcttgaa gtcctggaag atcacgccga tgttgcggcg
    26101 cagcttcggg accttcttgg gcgggatctc gccgagcttg cggccggcga tcagcacctc
    26161 gccgcgcgag ggctcgagct ccttcatcag caggcgcgtc agcgtggact tgccgcagcc
    26221 ggtcgggccg accaggaaga cgaactcgcc gcgggtgacc tgaagcgaca cgcggtcaac
    26281 ggcgagcgac ccgccctcgt agaccttcgt gacctcgcgc agctcgacca ccggcacgta
    26341 tgggcgctcg gcgtcggtgc gcaccgggcg tacgttctcg ctctcgacga ctgccatgag
    26401 ccgcctttaa tagctggcga acgcgccgca gccaaggcgt gcaacgggtc ctgcagtccg
    26461 tcctagggcg cgacgcggta gcggaggaac aggtcccccc cgccctcgag cgccgacacg
    26521 agctccagcc cggtcggcgg atcgagctcc ggccccgaca cgaccgaggg cccgccgccg
    26581 cccgccagct tcgacgcgac caccaggtac agctcgtcca cgaggccctc gcggaggagc
    26641 gtgccgttga gcaccgggcc gccctcgcag aggaccgtct cgacgccgcg ctcgcgcagc
    26701 tcctcgagcg ccggtcgcat cgggacgatc ccggcggcct cgtccttggg ggtgcggatg
    26761 tagtcgacgc tggcgagcac gccggcgatc gtctcgtcgg cggcggtcac gcacgtgacg
    26821 cacgagtcgg cgtcctggag cagggggacg ttctccgggt cgagggccag gcgcgcgctc
    26881 accaccacgg ccagtgggtc cgcctcgagg cccgcggcca cgcgacggtc gcgacggtcg
    26941 gggtcgcgga cgaggcgccc gtagcgctcg ttgcgcgcgg tgcccgcgcc gaccatcacc
    27001 gcgtcgacct gcgcgcgcag ctcgtggaag agcgcgcggt cgtgggcgtt gccgatcggg
    27061 ccggagcggc cgtcgatcgt gatgcggccg tcggcggtgg cgaccatgtt gagcaccagg
    27121 tacgggcgcg acgcgtgcgg gcgggcgccg gcgcgcagct ccgccgcggc ctcggtggcc
    27181 gtgagcgtgc cggtgctggg gaagaggctc tggaactcca cgcggaggag cgtattccga
    27241 ggagtgggcc gagccacctt ctggccggct gcgtataacc cggcgtgatc tttttcgccc
    27301 ctgtgcgtta ccgcagggga gggccgtgac cccacgccga ctcatcgaac ccgctcgtct
    27361 cgccggatcc gcgctgctgc acacgcagag cgacgagcgg ctggtcgacc tcgcgcggtc
    27421 gggcaacgac cgggccttcg aggcgatcgt ggcgcgctac cgccggccgc tcctgcggta
    27481 ctgccggaag ctgctgccac cggagcgggc cgaggatgcg gtgcagcagg cgttcctcaa
    27541 cgcgtaccgg gcgatcggcg cgggcgaggc cgagctgaat ctcaagccat ggctctaccg
    27601 gatcgcgcac aacgcttcgc tgagcctcct gcgccagaac ggctggagcc acgaggagct
    27661 cgacgagcag ttcgacggcg tgcagcagcc cccgcaggcg ttcgagcagc gcgagcaggt
    27721 gcgcgaggtg gttcgggcgg tgcaggggct cccggagcgc cagcgggatg ccgtcgtgct
    27781 gcgcgagctc gagggacgct cgtacgacga gatcgcggtg gccctcggcg tgagcggagg
    27841 agcggtccgg cagcttctca accgggcgcg gacgaatctg cgggctgcgg ccacggccct
    27901 tacgcccttc gacctcgtcg cctggtgcgt ctcccgtggc ggcgccggcg ccacgggagc
    27961 cgagcggatc gccgagttga cggcgggcgg cgcgggtggt cttgccgctg ccgcgaagct
    28021 cggaaccgct ctggtggttg ccggtgcggt ggcgggggcg ggcaccggag ttggaccgct
    28081 gccggacatg gtcggtggtg gcggggatgc aggggccgag gaggccgccg cggccgagcc
    28141 cgccggcggc ccggccggcg aggacggccc agccacccgc gtacggcatg gccgcggagc
    28201 cgatggccgc acggaggggc gcgggcgccg cggcggcgac gatcgcgctg gacgcgaccg
    28261 ctctcgaagc ggctcggacg acgactcggg atcccgcggc agcggctcgg gcggtgggtc
    28321 tgacgacgac gatggcggtt ccggttcgag tggctcaggc tcaggcgacg acgactcggg
    28381 ctcgggttcc agcggctcgg gcagcggcgg ctcggattcc agtggctcgg gttccagcgg
    28441 atcggactcg agcggctctg gctcgagcgg ctcgggcacc agcggctcgg gttccagcgg
    28501 ctccgggacg agcggctccg gctcggactc cagcggatcg ggcccggggc cggactcgcg
    28561 ttcgggctcg ggtgactccg gcacgagcgg ctccggctcc ggcacgagcg gctccggctc
    28621 cgggtcgggc tcgaattcgg gctccggctc cggcctcagt tccggctccg gctccgagta
    28681 gcactccggc tccggcctca gttccggctc cggcggcgag tagcactccg gctccgagcg
    28741 gcgccccggc gcgtcgctcc ccctcagcgt gagctttccg tcgccgctgc gttccaggag
    28801 cagacggccg ggatcgaccg gctcgtgaga cccccaagtt caaaccgagg agcacacgat
    28861 gaagaggacc atcctgagag gcaccgtcgt ggccgccgcg cttgcgctca tgctgccggc
    28921 cgccgcctcg gccagcagag gcgaccgcga cggcgacaag atgtccgacc gctgggaggg
    28981 caagcacggg ctgcgcgtcg gcaaggacga cgcccgccgc gatcccgatc gagaccggct
    29041 gtcgaacctc aaggagtttc ggctgaagac gaacccccgc cgcgccgaca ccgacggcga
    29101 cggcctgcga gacggcgacg agcgccgtac ccgcaacgac gcgcgcgacc gcgacagcga
    29161 cgacgacggc gtgcgcgacg gcgacgaggt gaacgggacc gtcgcgagct tcgactcggc
    29221 gacggg
//
LOCUS       DASVPL010000241         6910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_302984, whole genome shotgun sequence.
ACCESSION   DASVPL010000241 DASVPL010000000
VERSION     DASVPL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302984"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VF517_12955"
     CDS             complement(<1..891)
                     /locus_tag="VF517_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX8053895.1"
                     /translation="MLGRRTVVAALAASATLTAAPAAQALPVTVERAVERTCQARLLP
                     AGTPGVARETVAPKAAGVTTARLTGSAGDWDLALFDAKTGRLHNAGATAGRSEQAVAL
                     APANRAIVAQACRRTGSGASAELKIGFYQPPAPAAEREPPAIVHVPLGAGRTIAQLEA
                     TGLDVTHAVDASGADVVLYSAAERTRLLAAGFTYRTVVPDLRKADFASFRADRAYAGS
                     IRASALPSGRDSYRTLADYGTDLKGIVDKYPAVARRVEIGKSLEGRPIEGVELGGNVG
                     GRDGRPIFAVLGAHHAREWPS"
     gene            complement(928..2274)
                     /locus_tag="VF517_12960"
     CDS             complement(928..2274)
                     /locus_tag="VF517_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053896.1"
                     /translation="MLRALAAALVLAACLAAAAPAAADPAFLRQFGGQEGKAPGQFAL
                     PDGIGTDNAGNVWVADSQNNRIQRFSPTGEVLPFQAFAKRGWSQAPGRFKLPYDVDAD
                     ALGNLFVADTQNNRIQKFTPEGRFVRMWGRNGGDGTAGVGPGEMDQPRGLATDPFGNV
                     WVADHENARVLKFDNDGKLLLTLGANGGDGSYGEGVGEFNSPRGICSDSAGTIYVADD
                     VNHRIVKLANADGRVLATFGKPGGPASGFGTGPGEFRLPYGCAVDNRNRLWVADTNNN
                     RVVVLTTDGLFIRNWGVNGGDGTGGSGQGEFDEPYNAGGDCAGNIYISDEDNHRVQVF
                     GEPGSPRPVCPPGLTLSGGRASLGKRRIEIGAACDRICRVVARVSLRDTRGRLVRLTT
                     AEQILTGGAPARTLAIRFRSDLAKRFARGAKVSVRVTASGAPGADRTRPKTLTLGQ"
     gene            2307..3317
                     /locus_tag="VF517_12965"
     CDS             2307..3317
                     /locus_tag="VF517_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053897.1"
                     /translation="MRLPDRRGLIAPALIVALALATVYVQYRGLLVDSAGRDPQQFIE
                     VDLRDRHDAILDHTQGDPWRYRLLSEWGAEGSLRVADAGGFEKPAVVGFLGFRVLQNL
                     AIFWLMWLYLRRLGIGWYESLFGLGLLAWGFTQALFHQAMSFNTYGDIVFYLAAGLLI
                     LNRRWWWIVPLSVVAAVNRETSGLIPVMLMALAWPLGWRSEEGRRALRIGAVALAAFA
                     VSYGIVRLAVGSGFFIKADGQDPGLELLDYNVTRGQTWDFVFQTLNILPLLALLAWRR
                     WTPELRAFGLAIVPAWIAIHVFTSVLAESRLLLVPLALVFIPGVLAGLGAPRGEPEPA
                     RT"
     gene            complement(3253..3765)
                     /locus_tag="VF517_12970"
     CDS             complement(3253..3765)
                     /locus_tag="VF517_12970"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEX8053898.1"
                     /translation="MRPASDTLSEARSARHPSAAGWLLGIGLGGFVDGIVLHQILQWH
                     HLLSDEGSHPTTTVAGLEANTLADGLFHLATWLAVAAGLYLLWRDSQAGRTWIGRRLL
                     GLMLAGWGAFNLVEGIVDHHILTLHHVKEGDSELAFDLAFLALGAALLTFGLALARRA
                     ERPAPPARPG"
     gene            complement(3872..4270)
                     /locus_tag="VF517_12975"
     CDS             complement(3872..4270)
                     /locus_tag="VF517_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053899.1"
                     /translation="MTDARIEPVPADPAFARVFLEKAEGFLADADRVGLDSRQILCWQ
                     ACISMLEAILLAAGRRVTPGSGGHMRRLEEAERVLGGNHAELFERLDLHRDARHDVSY
                     AAGVASDLGTDELRQAAAELLAVARSYVTR"
     gene            complement(4263..4856)
                     /locus_tag="VF517_12980"
     CDS             complement(4263..4856)
                     /locus_tag="VF517_12980"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8053900.1"
                     /translation="MRQPVPPFLPLFRSEAQVRLLGALTLNPRRNWSSDELATAIGAP
                     RPSVHRELQRAVAAGLVDRDDTRRPHRFRAVTKSPLYRPLRSLLELTVGIESQLRQLF
                     EGEPNVDAALIHGSWARGQAGPDSDIDLLVVGRPDVTRLRRSTREIGRAVGRRIDTVV
                     LSPEEFRKRRAAGDGFLEKILAEPHVVLAGSPWGTYD"
     gene            complement(4920..5471)
                     /locus_tag="VF517_12985"
     CDS             complement(4920..5471)
                     /locus_tag="VF517_12985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8053901.1"
                     /translation="MTRSRLLLLLALLASVLALPACGDDDEGSGSATTETIPPADAAQ
                     PTQTAPPVVEGGDPKKLGQKPKVEVPEGDPPSKLETEDIVKGKGAVAKKGDSVTVQYV
                     GVLYDGGTEFDASWNRNEPFPFELGAGMVIPGWDEGVAGMKEGGRRKLIIPPDLAYGP
                     QGQPPTIPPNSTLVFVVDLLKVE"
     gene            5544..6140
                     /gene="folE"
                     /locus_tag="VF517_12990"
     CDS             5544..6140
                     /gene="folE"
                     /locus_tag="VF517_12990"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX8053902.1"
                     /translation="MIELLSSNGNGHAPAAIDVPRAERAAAELLAALGVDLEAESTRE
                     TPRRLVGVYSELLAPRPFNATTFTNDDGYDELVVARGIPFHSLCEHHLLPVTGVAHVG
                     YLPGERIIGLSKLARVVELFARRLQVQERLTKQVADWLERELAPRGVGVVLEAEHMCM
                     SLRGVQKPGASTVTSALGGLVRDDSRTRQEFLTLTGRS"
     gene            6184..6813
                     /locus_tag="VF517_12995"
     CDS             6184..6813
                     /locus_tag="VF517_12995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8053903.1"
                     /translation="MERAIDHRDLAPDSPSRAWIAALQADGPVRDQAIARLHELLLRG
                     ARFELARRRAALSHVRGEELEDIATQAADDALMAVLRKLDDFRGASRFTTWAYKFALL
                     EAGVRLRRRAWQDREVVLEPESWPAFADGGASPQRELEQAELLQALQRAVATALTPHQ
                     RSVFAALALNGVPIDVLAERLDTTRGALYKTLHDARRKLRAELEREGMA"
BASE COUNT          926 a         2463 c         2508 g         1013 t
ORIGIN      
        1 tgagggccac tcgcgcgcgt ggtgcgcgcc gaggacggcg aatatcggcc gcccgtcgcg
       61 cccgccgacg ttgccgccca gctccacgcc ctcgatcggc cggccctcga gcgacttgcc
      121 gatctcgaca cggcgcgcga ccgccgggta cttgtcgacg atccccttca ggtcggtccc
      181 gtagtcggcg agcgtgcggt acgagtcgcg gccgctcggc agcgcggatg cccggatcga
      241 tcccgcgtaa gcgcggtcgg cgcggaagct ggcgaagtcg gccttgcgca ggtcgggcac
      301 gaccgtgcga taggtgaacc cggccgcgag caggcgcgtg cgctcggcgg ccgagtagag
      361 gacgacgtcc gcgccgctcg cgtcgaccgc gtgcgtgacg tcgagcccgg tggcctcgag
      421 ctgcgcgatc gtgcgcccgg cgccgagcgg cacgtgcacg atcgcgggcg gctcgcgctc
      481 cgcggccgga gcgggcggct ggtagaagcc gatcttcagc tcggcgctcg cgccggagcc
      541 ggtgcgccgg cacgcctgcg ccacgatcgc gcggttcgcc ggcgccagcg cgaccgcctg
      601 ctcgctgcgg cccgcggtcg ccccggcgtt gtgcagccgg ccggtcttcg cgtcgaagag
      661 cgcgaggtcc cagtcgccgg cggagcccgt cagcctggcc gtggtcactc cggccgcctt
      721 cggcgcgacc gtctcgcgcg cgacgccggg cgtgccggcc ggcagcagcc gcgcctggca
      781 cgtgcgctcg accgcccgct cgacggtcac gggcagggcc tgcgcggcgg gtgcagcggt
      841 cagcgtcgcc gatgcggcga gtgcggcaac cacggtgcgc ctcccgagca cgcgcgaaag
      901 actacggatc ggcagtcggg ccggcgccta ctgcccgagc gtcagggtct tcggccgcgt
      961 gcgatccgcg cccggcgcgc ccgacgcggt cacgcggacc gacaccttcg ccccgcgcgc
     1021 gaagcgcttc gccaggtcgc tccggaaccg gatcgccagc gtccgcgccg gcgcgccccc
     1081 ggtcaggatc tgctcggccg tcgtgagccg cacgaggcgc ccgcgcgtgt cgcgcaggct
     1141 cacccgcgcg accacccggc agatccggtc gcacgcggcg ccgatctcga tccggcgctt
     1201 cccgaggctc gccctgccgc cgctcaaggt aagccccggc gggcacaccg gccgcgggct
     1261 gcccggctcg ccgaacacct ggacccggtg gttgtcctcg tcgctgatgt agatgttgcc
     1321 ggcgcagtcg ccgcccgcgt tgtagggctc gtcgaactcg ccctggccgg agccgccggt
     1381 cccgtcgccg ccgttcacgc cccagttgcg gatgaacagc ccgtcggtgg tgagcacgac
     1441 cacgcggttg ttgttcgtgt cggccaccca gaggcggttg cggttgtcga ccgcgcagcc
     1501 gtacgggagc ctgaactcgc ccgggccggt gccgaacccg ctggccggcc cgccgggctt
     1561 gccgaaggtc gccagcaccc gcccgtcggc gttggcgagc ttcacgatgc ggtggttcac
     1621 gtcgtcggcg acgtagatcg tgccggcgga gtccgagcag atcccgcgtg gggagttgaa
     1681 ctcaccgacg ccctcgccgt agcttccgtc gccgccgttg gcgccgagcg tgagcagcag
     1741 cttgccgtcg ttgtcgaact tcaggacgcg ggcgttctcg tggtcggcca cccagacgtt
     1801 gccgaacggg tcggtggcga gcccgcgcgg ctggtccatc tcgcccgggc cgacaccggc
     1861 cgtgccgtcg ccgccgttgc ggccccacat gcgcacgaac ctgccctccg gcgtgaactt
     1921 ctggatgcgg ttgttctggg tgtcggcgac gaagaggttg ccgagcgcgt ccgcgtccac
     1981 gtcgtacggg agcttgaagc gtccgggcgc ctggctccag ccgcgcttgg cgaacgcctg
     2041 gaacggcagc acctcgcccg ttgggctgaa gcgctggatc cggttgttct ggctgtcggc
     2101 gacccagacg ttgcccgcgt tgtcggtccc gatcccgtcc ggaagggcga actggcccgg
     2161 cgccttgccc tcctggccgc cgaactggcg caggaaggcc gggtcggctg ccgcgggcgc
     2221 ggcggccgcc aggcaggcgg cgaggacgag cgctgcggcg agtgcgcgaa gcacggcgcg
     2281 gcaggttagc gcgctacctt cgcgccatgc gcctgcccga tcgcaggggg ctgatcgctc
     2341 cggcgctcat cgtcgcgctg gcgctggcca ccgtctacgt ccagtaccgc ggcctgctcg
     2401 tggactcggc cggacgcgac ccgcagcagt tcatcgaggt ggacctgcgc gatcgccacg
     2461 acgcgatcct cgaccacacc cagggcgatc cctggcgcta ccgcctgctg tcggagtggg
     2521 gggcggaggg gtcgctgcgc gtggccgacg cgggcggctt cgagaagccc gccgtggtcg
     2581 gcttcctcgg cttccgcgtg ctccagaacc tcgcgatctt ctggctgatg tggctctacc
     2641 tgcgccggct cgggatcggc tggtacgaga gcctgttcgg actcgggctg ctggcgtggg
     2701 gcttcaccca ggcgctcttc caccaggcga tgtcgttcaa cacctacggc gacatcgtgt
     2761 tctacctggc ggcgggcctg ctgatcctga accgcaggtg gtggtggatc gtgccgctca
     2821 gtgtggtcgc ggcggtgaac cgggagacga gcgggttgat cccggtgatg ctgatggccc
     2881 tggcgtggcc gctcggctgg cgcagcgagg agggtcgccg ggcgctgcgg atcggggcgg
     2941 tcgcgctcgc cgccttcgcg gtcagctacg ggatcgtcag gctcgcggtg gggtcgggct
     3001 tcttcatcaa ggccgacggg caggaccccg gactcgagct gctcgactac aacgtcacgc
     3061 gcggccagac gtgggacttc gtcttccaga cgctgaacat cctgccgctg ctcgccctgc
     3121 tggcgtggcg gcgctggacg ccggagctga gggcgttcgg gctggcgatc gtccccgcct
     3181 ggatcgcgat ccacgtgttc acctcggtgc tggcggagtc gcgcctgctg ctcgtgccgc
     3241 tggcgctcgt gttcatcccg ggcgtgctgg cggggctggg cgctccgcgc ggcgagccag
     3301 agccagcccg aacgtgagca gcgccgcgcc cagcgcgagg aacgcgaggt cgaacgcgag
     3361 ctccgagtcg ccctccttca cgtggtgcag ggtgaggatg tggtggtcga cgatgccctc
     3421 gaccaggttg aacgcgcccc atccggccag catgaggccg agcaggcggc ggccgatcca
     3481 cgtgcggccc gcctggctgt cacgccacag caggtagagg ccagccgcca ccgcgagcca
     3541 ggtggcgagg tggaacaggc cgtcggcgag cgtgttcgcc tcgagccccg cgaccgtcgt
     3601 ggtcggatgg ctcccctcgt cgctcagcag gtggtgccac tgaaggatct ggtgcagcac
     3661 gatgccgtcg acgaacccgc cgaggccgat cccgaggagc cagccggcgg cgctggggtg
     3721 acgggccgag cgagcctctg agagcgtgtc gctggctggg cgcacgcgga gcacctaccc
     3781 gatagccact cgtacgaccc gcgatgcatc gcgaatcccg aagccgaagg tcgggggttc
     3841 gagccccgtc gcacgcagtg cagactcgcg gtcagcgcgt cacgtaggag cgagcaaccg
     3901 ctagcagttc agccgccgcc tgcctcagtt cgtcagttcc gagatcggac gcgacacccg
     3961 cggcgtagga gacgtcgtgc cgcgcgtcgc gatggaggtc gagccgctcg aatagctcag
     4021 cgtggttgcc tccgagcacc cgctcggcct cctcgaggcg gcgcatgtgt ccgcctgaac
     4081 caggagtcac ccggcgaccg gcggcaagca ggatcgcctc gagcatcgag atgcacgctt
     4141 gccagcagag gatctgcctc gagtcgagcc cgactcgatc cgcatcggca aggaagccct
     4201 ccgctttctc gaggaagact cgtgcgaacg cgggatctgc gggcacgggt tcgatgcgcg
     4261 cgtcagtcat aggtgcccca ggggcttccc gcgaggacga catgcggctc cgccaggatc
     4321 ttctccagaa atccgtcgcc ggcggcgcgg cgcttgcgga actcctcagg cgagaggacg
     4381 acggtgtcga tccggcgccc gacggcccga ccgatctcgc gagtcgagcg ccgcagccgg
     4441 gtcacgtcgg gccggccgac cacgaggagg tcgatgtcgc tgtcgggacc tgcctggccg
     4501 cgagcccacg agccatgaat caaagcggcg tcgacattcg gctcgccctc gaagagctga
     4561 cgaagctgcg actcgatccc gaccgtcagc tccagcagac tgcgcagcgg ccggtacagc
     4621 ggacttttcg taacggctcg aaagcggtgg ggtcgccgag tatcgtcgcg atcgactagg
     4681 cccgccgcca cagcccgctg cagctctcgg tgcaccgagg gccgaggcgc cccgatcgcc
     4741 gtggcgagtt cgtcggacga ccaatttcgc cgcgggttga gggtcagggc ccctagcagt
     4801 cgaacctgcg cctccgatcg aaaaagcggc aagaacggag gcactggctg tcgcattact
     4861 gagatttata tctcaataat cagacggcca attcccaact gggggtagtt tcgggtgacc
     4921 tactcgacct tcagcaaatc cacgacgaac accagcgtgg agttcggcgg gatcgtcggc
     4981 ggctggccct gcgggccgta ggcgaggtcg ggcgggatga tcagcttgcg ccgcccgccc
     5041 tccttcatgc ccgccacgcc ctcgtcccag ccggggatga ccatccccgc gccgagctcg
     5101 aacgggaacg gctcgttcct gttccaggac gcgtcgaact cggtgccgcc gtcgtagagc
     5161 acgccgacgt actgcacggt cacgctgtcg cccttcttcg cgacggcgcc cttgcccttg
     5221 acgatgtcct cggtctcgag cttcgacggc gggtcgccct ctgggacctc gaccttcggc
     5281 ttctgcccga gcttcttggg gtcgccgccc tcgaccacgg ggggcgcggt ctgggtcggc
     5341 tgggccgcgt cggcgggtgg gatcgtctcg gtggtggcgg agccgctgcc ctcgtcgtcg
     5401 tccccgcagg cggggagggc gagcacggag gcgagcaggg cgagcaggag caggaggcgt
     5461 gagcgtgtca tcggcgcgag gctacaacgc tcgcccccgg cgacggaggt aagaaccgcc
     5521 cgcgcgggcg caccaagggg ttcatgatcg aactcctgag cagcaacgga aacggacacg
     5581 cgcccgctgc gatcgacgtg ccgcgggcgg agcgtgcggc ggccgagctc ctggccgcgc
     5641 tcggtgtgga cctcgaggcc gagagcacgc gggagacgcc gcggcggctc gtcggcgtct
     5701 acagcgagct gctggcgccg cgccccttca acgccaccac gttcaccaac gacgacggct
     5761 acgacgagct cgtggtcgcg cgcgggatcc cgttccactc cctctgcgag caccacctgc
     5821 tgccggtcac cggcgtcgcg cacgtgggct acctgccggg cgagcggatc atcggcctgt
     5881 cgaagctcgc ccgggtggtc gagctgttcg ctcggcggct ccaggtgcag gagcggctga
     5941 ccaagcaggt ggccgattgg ctcgagcgcg agcttgctcc gcgcggggtc ggcgtggtgc
     6001 tcgaggccga gcacatgtgc atgtcgctgc gcggcgtgca gaagcccggc gccagcaccg
     6061 tcacctccgc gctgggcggc ctcgtgcgcg acgactcgcg cacccgccag gagttcctga
     6121 cgctcacggg gcggagctag ccggatgacc gcggtcgcgc tcgcgccgac ctatgctcgc
     6181 cacgtggagc gcgcaatcga ccaccgcgac ctcgctcccg acagcccctc ccgcgcgtgg
     6241 atcgcggcgc tccaagccga cgggcccgtg cgcgaccagg cgatcgctcg gctgcacgag
     6301 ctgctgctgc gcggcgcgcg gttcgagctc gcgcggcggc gggccgcgct ctcgcacgtg
     6361 cgcggcgagg agctcgagga catcgcgacg caggcggcgg acgacgcact gatggccgtg
     6421 ctccgcaagc tcgacgactt ccgcggcgcg agccgcttca ccacctgggc ctacaagttc
     6481 gcgctgctcg aggccggcgt gcggctgcgc cgccgcgcct ggcaggaccg cgaggtcgtg
     6541 ctcgagcccg agagctggcc ggcgttcgcg gacggcggcg cctcgccgca gcgggagctc
     6601 gagcaggcgg agctgctgca ggcgctccag cgcgccgtcg cgaccgcgct cacgccgcac
     6661 cagcgcagcg tgttcgccgc gctcgcgctc aacggggtgc cgatcgacgt gctcgccgaa
     6721 cgtctcgaca ccactcgcgg cgcgctctac aagactcttc acgacgcccg ccggaagctc
     6781 cgcgcggaac tcgaacggga gggaatggca tgagcgacga cgctcgcgaa cggctcgccc
     6841 ggctgctggg gcccgcgggg cccgaggtct cctgcgagac ctgcttcgag gtgctcgacg
     6901 agtacgtgga
//
LOCUS       DASVPL010000242        10606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_382976, whole genome shotgun sequence.
ACCESSION   DASVPL010000242 DASVPL010000000
VERSION     DASVPL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382976"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..359
                     /gene="rpsJ"
                     /locus_tag="VF517_13000"
     CDS             36..359
                     /gene="rpsJ"
                     /locus_tag="VF517_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX8053904.1"
                     /translation="MAALTQNKIRIRLKAYDHSAIETAAREIVETATRTGATVSGPVP
                     LPTEKNVYCVIRSPFKDKDSREHFEIRTHKRLIDILQPTPKTVDSLQRLDHLPSGVDI
                     QIQLV"
     gene            364..981
                     /gene="rplC"
                     /locus_tag="VF517_13005"
     CDS             364..981
                     /gene="rplC"
                     /locus_tag="VF517_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX8053905.1"
                     /translation="MAALLGRKLGMTQVFSEDGTVQRVTVVEAGPCHVTGVRSFDRDG
                     YEAVQLAFGDIKEKNLTKAERGHLAKADAPALRRVKEFRDEAGELTVGEVVRADVFQV
                     GDRVKISGISKGKGFQGTIKRHNFSRGPVSHGSHNVRAPGSIGASATPSRVFKGIRGP
                     GQMGNKRVTQKGVEVVDVRPDENLLLVRGSVPGPRGQIVEIRTDS"
     gene            981..1643
                     /gene="rplD"
                     /locus_tag="VF517_13010"
     CDS             981..1643
                     /gene="rplD"
                     /locus_tag="VF517_13010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX8053906.1"
                     /translation="MAAPKAPYLGKSGSVDLDTEVFGEEFHMALVHEAVKAELNARRR
                     GTASTLTRGEVAMTTAKAFRQKGTGRARAGALSTPQRTGGGIAFGPKPRGYKVKVNRK
                     ARRRAMRAALTQHAERGTIAVVDPADFDAPATKRAAEGLAGFGEGHALVVLAREGEET
                     AVKSFRNLTGVTVLPSDNVGVSDVVRARRLIVSPSALERITTVAKPPTMSSHVERTGA
                     AA"
     gene            1640..1936
                     /gene="rplW"
                     /locus_tag="VF517_13015"
     CDS             1640..1936
                     /gene="rplW"
                     /locus_tag="VF517_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX8053907.1"
                     /translation="MNARSVLIKPIVSEKSYALLAANKYTFRVHSDAHKTQIRQAVEE
                     IFGVRVLEVQTMSVKPKPKRRGWSSGHTRAWKKAIVKLHDEDSIELFEGQELAE"
     gene            2061..2903
                     /gene="rplB"
                     /locus_tag="VF517_13020"
     CDS             2061..2903
                     /gene="rplB"
                     /locus_tag="VF517_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX8053908.1"
                     /translation="MALKRYKPTSPGRRFATFQDNSEVTKKEPEKRLTQGIKKSGGRN
                     THGRITSRHRGGGAKRRYRMVDFKRRKDGVPARVAAIEYDPNRSAYIALLNYADGEKA
                     YILAPARLRVGATVVSGPGAEIQPGNCLPLRNIPTGTTIHNVELEPGRGGQLARSAGA
                     AVQLMAKEGARATLRLPSGEMRTVPIEARASIGAIGNADHQNISVGKAGRSRHKGKRP
                     QTRGTAMNPVDHPHGGGEGSTTPGRHPVTPWGVPTLGYPTRKKRKESDRYIVRRRRRG
                     KGKR"
     gene            2910..3188
                     /gene="rpsS"
                     /locus_tag="VF517_13025"
     CDS             2910..3188
                     /gene="rpsS"
                     /locus_tag="VF517_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX8053909.1"
                     /translation="MSRSSKKGPWVEERLIDRIEAMNSSGNKQMIKTWSRASTIFPEM
                     VGHTIAVHDGRKHVPVFISESMVGHKLGEFAPTRLFRGHAGGDKARMR"
     gene            3524..3892
                     /gene="rplV"
                     /locus_tag="VF517_13030"
     CDS             3524..3892
                     /gene="rplV"
                     /locus_tag="VF517_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX8053910.1"
                     /translation="MTVRAKAKYVRTAPRKARLVIDHIRGKSVDDARALLLTTPRAAS
                     RDVLKLLDSAIANAENNHELVADELVVGRAYVDEGPTLKRYRPRALGRATRIRKRTSH
                     MTIELTNQIENGRRRNGGKR"
     gene            3895..4521
                     /gene="rpsC"
                     /locus_tag="VF517_13035"
                     /pseudo
     CDS             3895..4521
                     /gene="rpsC"
                     /locus_tag="VF517_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            5046..5468
                     /gene="rplP"
                     /locus_tag="VF517_13040"
     CDS             5046..5468
                     /gene="rplP"
                     /locus_tag="VF517_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX8053911.1"
                     /translation="MLQPKRVKHRKQHRGRRAGYAKGGTRVEFGEYGLKALDRGWITN
                     RQIEAARIAMTRKIKRGGKVWINIFPDKPITQKPAETRMGSGKGSPEGWVAVVKPGRV
                     MFELAGVPEDLAREAMRLAGHKLPVKTKFVMREGAGPQ"
     gene            5489..5692
                     /gene="rpmC"
                     /locus_tag="VF517_13045"
     CDS             5489..5692
                     /gene="rpmC"
                     /locus_tag="VF517_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX8053912.1"
                     /translation="MADDQLAEFVQRSRQEVFNLRFQHATGQLENTSRMGQAKRDLAR
                     GLTIARQRNLDIDAELRRQRDSA"
     gene            5865..6098
                     /gene="rpsQ"
                     /locus_tag="VF517_13050"
     CDS             5865..6098
                     /gene="rpsQ"
                     /locus_tag="VF517_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX8053913.1"
                     /translation="MRQGVVISDKADKTISVKIDIARRHRTYKKIVRHSTTLHAHDER
                     NDAHTGDTVRVVESRPLSRTKRWRLVEVVERAR"
     gene            6262..6630
                     /gene="rplN"
                     /locus_tag="VF517_13055"
     CDS             6262..6630
                     /gene="rplN"
                     /locus_tag="VF517_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX8053914.1"
                     /translation="MIQPETRLRVADNTGAREILCIRVRGGSHRRYAGVGDIIVGTIK
                     QATPHGSVRKGEVVTAVVVRTRKQFGRDDGTYIAFDENAAVLIDPQNNPRGTRVFGPV
                     ARELRDRNFMKIVSLAPEVL"
     gene            6635..6970
                     /gene="rplX"
                     /locus_tag="VF517_13060"
     CDS             6635..6970
                     /gene="rplX"
                     /locus_tag="VF517_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004268591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX8053915.1"
                     /translation="MPSRIRKDDVVKVISGKDKGKTGRVLRVEPRRDRVFVEGLNIQK
                     RHTRPRTIRDASKGGQVGGVVEKEGPIHISNVMPLDPGNGEATRVSHTIEEDGRRVRV
                     GKRSGTAFE"
     gene            6991..7530
                     /gene="rplE"
                     /locus_tag="VF517_13065"
                     /pseudo
     CDS             6991..7530
                     /gene="rplE"
                     /locus_tag="VF517_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            7773..7958
                     /locus_tag="VF517_13070"
     CDS             7773..7958
                     /locus_tag="VF517_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX8053916.1"
                     /translation="MAKTSQVVRQQRPAKYKTRQYNRCRRCGRPRAYFRKFGLCRICL
                     REMAHEGYVPGLTKSSW"
     gene            7972..8370
                     /gene="rpsH"
                     /locus_tag="VF517_13075"
     CDS             7972..8370
                     /gene="rpsH"
                     /locus_tag="VF517_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010169919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX8053917.1"
                     /translation="MSMTDPIADFLTRIRNGLRADRDAVDIPASRFKVEIARILREQG
                     YIEDYSVEPAEVGEVVRVRLKYTQDRAPVISGIQRVSRPGRRTYVNATEIPKVLGGMG
                     TSIVSTSKGVMTGHDARRQGVGGEVVAYVW"
     gene            8378..8911
                     /gene="rplF"
                     /locus_tag="VF517_13080"
     CDS             8378..8911
                     /gene="rplF"
                     /locus_tag="VF517_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX8053918.1"
                     /translation="MSRIGKQPVPFPSTVDVTIEPEVVRVNGPRGALEERVSRDLTIE
                     KNDGEIVVTRSTNRGEHRALHGLTRSLIANMVQGVTEGFEKRLEIQGVGYRAALRGKD
                     IDLQVGYSHPVSIKAPDGIEFEVPVPTQIIVRGNSKQAVGEIAAQIRKVRPPEPYKGK
                     GIRYAGEHVMRKVGKRA"
     gene            8908..9261
                     /gene="rplR"
                     /locus_tag="VF517_13085"
     CDS             8908..9261
                     /gene="rplR"
                     /locus_tag="VF517_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX8053919.1"
                     /translation="MSVKTRQQARLRRRRRVRAKVRGSAERPRLSVFRSNKGIQAQLI
                     DDDAGRTLAAVNWVESDLRELKPEEQAKRVGQLLAERASAAGVETCVFDRGGYLYHGR
                     VRALAEAAREAGLRF"
     gene            9295..9816
                     /gene="rpsE"
                     /locus_tag="VF517_13090"
                     /pseudo
     CDS             9295..9816
                     /gene="rpsE"
                     /locus_tag="VF517_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            9930..10103
                     /gene="rpmD"
                     /locus_tag="VF517_13095"
     CDS             9930..10103
                     /gene="rpmD"
                     /locus_tag="VF517_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX8053920.1"
                     /translation="MTLKLTQVKSANGTNPKQKGALRSLGLGRIGKTTERPDDPTVRG
                     AVQSVRHLLRVEE"
     gene            10100..10585
                     /gene="rplO"
                     /locus_tag="VF517_13100"
     CDS             10100..10585
                     /gene="rplO"
                     /locus_tag="VF517_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX8053921.1"
                     /translation="MSEEIGLHNLKPAPGSRHRRKRVGRGEGSGVGKTSGRGQKGYGS
                     RAGSKSRPGAEGGQMPIHMRMRKLRGPHMKKSMPFEPFRTQTQPVNLRDLDARFGDGD
                     SVTPHTLKVHGLATRTGVPVKILGVGELSKKLTVSAHGFSKSAREKIEGAGGTCTVLD
                     D"
BASE COUNT         1815 a         3766 c         3760 g         1265 t
ORIGIN      
        1 caggcgaagc tgggtccgcg acgagcatcc acaccatggc cgcacttacg cagaacaaga
       61 tccggatccg gctgaaggcc tacgaccact cggcgatcga gacggccgcg cgcgagatcg
      121 ttgagaccgc gacgcgcacc ggcgcgacgg tgtcggggcc ggtcccgctg ccgaccgaga
      181 agaacgtgta ctgcgtgatc cgcagcccgt tcaaggacaa ggactcgcgc gagcacttcg
      241 agatccgcac gcacaagcgg ctcatagaca tcctccagcc caccccgaag accgtcgact
      301 cgctccagcg gctcgaccac ctcccgtcgg gcgtggacat ccagatccag ctcgtctagg
      361 ccgatggctg cgctgctcgg cagaaagctc gggatgaccc aggtcttctc tgaggacgga
      421 acggtccagc gggtaacggt cgtcgaggcc ggtccgtgcc acgtgaccgg tgtccgcagc
      481 ttcgatcgcg acggctacga ggccgtgcag ctcgcgttcg gggacatcaa ggagaagaac
      541 ctcacgaagg ccgagcgtgg gcatctcgcg aaggccgacg cgccggcgct gcgccgcgtc
      601 aaggagttcc gcgacgaggc gggcgagctg accgtcggcg aggtcgtgag ggccgacgtc
      661 ttccaggtcg gcgaccgggt caagatctcc gggatctcga agggcaaggg cttccagggc
      721 accatcaagc gccacaactt ctcgcgcggc ccggtctcgc acggctcgca caacgtgcgc
      781 gctcccggct cgatcggcgc ctccgccacg ccctcgcgcg tgttcaaggg catccgtggc
      841 cccggccaga tgggcaacaa gcgcgtcacg cagaagggcg tcgaggtcgt ggacgtgcgg
      901 cccgacgaga acctgctgct cgtgcgcggg tcggtccccg gcccgcgcgg tcagatcgtc
      961 gagatcagga cggactcgta gtggccgcgc ccaaggctcc ctacctcggc aagtccggca
     1021 gcgtcgacct cgacaccgag gtcttcggcg aggagttcca catggcgctc gtccacgagg
     1081 ccgtcaaggc cgagctgaac gcacgccgcc gcggcaccgc ctccacgctg acccgtggcg
     1141 aggtggcgat gaccaccgcc aaggccttcc ggcagaaggg caccggccgc gcccgcgccg
     1201 gcgcgctctc gaccccgcag cggaccggcg gcggcatcgc gttcggcccg aaaccgcgcg
     1261 gctacaaggt gaaggtcaac cgcaaggccc gccggcgcgc catgcgcgcg gcgctgaccc
     1321 agcacgccga gcgcgggacg atcgccgtgg tcgatcccgc ggacttcgac gcgcccgcca
     1381 ccaagcgcgc cgccgagggg ctcgccggct tcggcgaggg ccacgcgctc gtggtgctcg
     1441 ctcgcgaggg cgaggagacc gcggtcaagt ccttccgcaa cctcaccggc gtcacggtgc
     1501 tgccgtccga caacgttggc gtctccgacg tggtgcgcgc acggcggctg atcgtctcgc
     1561 cgagcgcgct cgagcggatc accacggtcg cgaagccgcc cacgatgtcg tcgcacgtcg
     1621 agcgcacggg ggccgcggca tgaacgcccg ctccgtcctc atcaagccga tcgtctcgga
     1681 gaagagctac gcgctgctcg cggccaacaa gtacaccttc cgcgtccact cggacgcgca
     1741 caagacgcag atccgccagg cggtagagga gatcttcggt gtgcgcgtgc tcgaggtgca
     1801 gacgatgagc gtgaagccga agcccaagcg ccgcggctgg agctccggtc acacgcgcgc
     1861 ctggaagaag gcgatcgtga agctgcacga cgaggacagc atcgaactct tcgagggcca
     1921 ggagctcgcc gaatgaccct gcaaaccttc gcatccccgc gtcctcgggt ctcgccgtgc
     1981 gttgcacggc ttcaaccctg cgtcctcggt ctgctcggtt ttcgggccca cgatcgagct
     2041 cctacggagc tggccgagta atggccctca agcgctacaa gccgaccagc cccgggcgcc
     2101 gcttcgcgac gttccaggac aactccgagg tgaccaagaa ggagccggag aagcggctca
     2161 cccagggcat caagaagagc ggcgggcgca acacgcacgg gcggatcacc tcgcgccacc
     2221 gcggcggcgg ggccaagcgc cgctaccgca tggtcgactt caagcgccgc aaggacggcg
     2281 tgcccgcgcg cgtggccgcg atcgagtacg acccgaaccg cagcgcctac atagcgctcc
     2341 tcaactacgc cgacggcgag aaggcataca tcctcgcgcc ggcgcggctg cgggtggggg
     2401 cgacggtcgt gagcgggccc ggcgccgaga tccagcccgg caactgcctg ccgctgcgca
     2461 acatcccgac cggcaccacg atccacaacg tcgagctcga gccgggccgt ggcgggcagc
     2521 tcgcccgctc cgccggcgcg gccgtccagc tcatggcgaa ggagggcgcg cgggcaacgc
     2581 tgcgcctccc gtccggcgag atgcgcacgg tgccgatcga ggcgcgcgcg agcatcgggg
     2641 cgatcggcaa cgccgaccac cagaacatct ccgtggggaa ggccggtcgc agccggcaca
     2701 agggcaagcg cccgcagacg cgcggcaccg cgatgaaccc ggtggatcat ccgcacggcg
     2761 gcggcgaggg atcgaccacg cccggccggc acccggtcac gccgtggggc gtcccgacgc
     2821 tcggctaccc cacacgcaag aagcgcaagg aatcggatcg ctacatcgtc cgccgccgca
     2881 ggcgcgggaa gggcaagagg taacgggtca tgtcacgttc ttccaagaag gggccgtggg
     2941 tcgaggagcg cctgatcgat cggatcgagg cgatgaactc cagcggcaac aagcagatga
     3001 tcaagacgtg gtcgcgcgcg tccacgatct tccccgagat ggtcgggcac acgatcgcgg
     3061 tgcacgacgg ccgcaagcac gtgccggtct tcatctccga gtcgatggtc gggcacaagc
     3121 tcggcgagtt cgctccgacg cgactgttcc gcggccacgc cggcggtgac aaggcgagga
     3181 tgcgttgatc gacgaggagc gcaaggacga ggcgaccgac gccgaggtgg aggccgccga
     3241 ggacgagtcg cgcgccgccg aggccgaggc cgaggcgggc gaggtcgcgg aggacgccga
     3301 ggcgaccgag ccggaggcga agcccaagcg ccgccgccgc aagaaggccg acgacgtgaa
     3361 ggaggaggcc gccgccgagg acgccgaggc tgaggccgcc gaggtcgacg gcgagccccc
     3421 cgccgaggac gagaaggcgc ggcccaagcg ccgccgcggc gccgccgagg tcacggcggg
     3481 cgccaagaag ccggcccgcc ggcgccgctc gcccgaggag gtcgtgaccg tccgcgcgaa
     3541 ggccaagtac gtgcgcaccg ccccgcgcaa ggcgcggctc gtgatcgacc acatccgcgg
     3601 caagtccgtc gacgacgcgc gtgctttact cctgaccacg ccgcgcgcgg cttcgcggga
     3661 cgtcctgaag ctcctggact ccgcaatcgc caacgcggag aacaaccacg aactggtcgc
     3721 cgacgagctc gtggtgggcc gggcctacgt cgacgagggc ccgacgctca agcgctaccg
     3781 tccgcgcgcg ctggggcggg ccacacgaat tcgcaagcgg acgtcgcaca tgaccatcga
     3841 gctcaccaac cagatcgaga acggccgccg ccggaacggg ggcaagcgct agccatgggt
     3901 cacaaggtcc gcccggagat cctgcgcgtc ggctacatcc acgactggaa gtcgacctgg
     3961 ttcaacgaga aggagttctc ggactacctg atcgaggacg tccgcatccg cgatcacatc
     4021 gagaccaagc tggcccacgc cggcctctcc gacatcacga tccgcaagga cacgaacgag
     4081 gtcgaggtca acatcaacac ggcccggccc ggcatcgtga tcggcaagtc cggcagcgag
     4141 gtcgacgcgc tccgccgcga gcttcacaag atgaccggca agtcggtcaa ggtgaacatc
     4201 ctcgagatca agcgccccga gctcgactcg aagctcgtcg cgcagtcggt gggcgagcag
     4261 ctccagaacc gcgtcgcctt ccgccgcgcg atgaagcgcg cgctcacctc cgccatgcgt
     4321 tccggcgcga agggcgtgaa gatccaggtc tccggccgcc tcggaggcgc cgagatggcg
     4381 cgcaccgagg gctactcgga cggccgcgtc ccgctgcaca ccctgcgcgc cgacatcgac
     4441 ttcggcttct acgaggcgcg caccaccttc ggccggatcg gcgtgaaggt ctggatcaac
     4501 aagggcgaga tcatgccctc cggcttcgcg caggacctca ccgagatcga ggcgccgcag
     4561 caggcgcgcg tcggcagcgg cggtcccggt ggccgcggcg gtcccggcgg acccggcggt
     4621 cgcggcggcg gcccgggtgg tcgcggcggc ggccccggtg gacgtggcgg ccccggcgga
     4681 cccggcggtc gcggtggcgg cccgggtggt cgcggcggcg gtccgggtgg tcgcggaggc
     4741 ggcggcggac gcgggcccgg tggccccggc ggtcgtggtg gctccggcgg cggtccgggt
     4801 ggaccgggcg gtcgcggtgg ctctggcggc ggtggacgcg gacccggcgg tcccggcggc
     4861 gggcgtggtc ccggcggcgg tggcgggcgc ggtgccggcg gtcccggtgg cggcggcggc
     4921 cgtggagccg gcggctccgg cggcggcccc agccgtggcg gtcagggtgc tcagtccggc
     4981 ccgcggcccc agggtggcga gggcggcgcg agcggcggca cgatggatcc gagccgaggt
     5041 agctgatgct ccagccgaag cgagtcaagc accgcaagca gcacagggga cgcagggccg
     5101 gctacgccaa gggcggcacg cgcgtcgagt tcggcgagta cgggttgaag gctctcgacc
     5161 gcggctggat caccaaccgc cagatcgagg ccgcccgaat cgccatgacc cgcaagatca
     5221 agcgcggcgg caaggtctgg atcaacatct tcccggacaa gccgatcacg cagaagccgg
     5281 ccgagacccg catgggctcc ggcaagggct cgcccgaggg ctgggtcgcc gtggtcaagc
     5341 cgggccgcgt gatgttcgag ctcgccggcg tgccggagga cctcgctcgc gaggcgatgc
     5401 gcctggccgg ccacaagctt cccgtcaaga ccaagttcgt catgcgcgag ggggctggtc
     5461 cccagtgaag ggcgcggagg tacgcgacat ggccgacgac caactcgccg agttcgtaca
     5521 gcgctcgcgc caggaggtct tcaacctgcg cttccagcat gccaccggcc agctcgagaa
     5581 cacgagccgg atggggcagg ccaagcgcga tctcgcccgc ggtctcacga tcgcgcggca
     5641 gcgcaacctc gacatcgacg ccgagctgag gaggcagcgc gacagtgcct gacgaggcga
     5701 ccatcaagca gcgcgtggag gagcgtgagg agcgccggcg caagaacgcc gccgcccgca
     5761 gccgcacgcg cgcgaagcag aaggagaagc gggccgccac gcgagccgag gctccgcccg
     5821 agccggccgt tgagggcaag cagcacggcc ccggccgccc gaaggtgcgc cagggcgtgg
     5881 tgatctcgga caaggccgac aagacgatca gcgtcaagat cgacatcgcc cgccgtcacc
     5941 gcacctacaa gaagatcgtc cgccactcga ccaccctgca cgcgcacgac gagcgcaacg
     6001 acgctcacac gggcgacacc gtgcgcgtgg tcgagagccg gccgctcagc cgcaccaagc
     6061 gctggcggct cgtcgaggtc gtggagaggg cgcgctagtg aggccctccg ctcactcgac
     6121 cggcactgcc gcggctgcga acactgcgcc cggcgtcgtc ctcggtcgcg cgaatagcgc
     6181 tgctattcgc cgctccctgc gtcctacccg ggcttggttc tcgctcgcgt cagcaccagt
     6241 cgagctcacg gaggacctcc gatgatccag ccggagacca ggctgcgcgt cgccgacaac
     6301 accggcgccc gcgagatcct ctgcatccgc gtccgcggcg gctcgcaccg ccgctacgcc
     6361 ggcgtcggcg acatcatcgt cggcacgatc aagcaggcca cgccgcacgg ctcggtccgc
     6421 aagggcgagg tcgtgaccgc ggtcgtggtc cgcacgcgca agcagttcgg ccgcgacgac
     6481 ggcacctaca tcgcgttcga cgagaacgcc gccgtgctga tcgacccgca gaacaacccg
     6541 cgcggaaccc gcgtcttcgg ccccgtcgcg cgcgagctgc gcgaccgcaa cttcatgaag
     6601 atcgtctcgc tcgctccaga ggtcctctag gccgatgccc tcgcgcatcc gcaaggacga
     6661 cgtggtgaag gtcatctccg gcaaggacaa ggggaagacc ggccgcgtgc tgcgcgtcga
     6721 gccgcgccgc gaccgcgtgt tcgtggaggg cctgaacatc cagaagcggc acacgcgccc
     6781 gcgcacgatc cgcgacgcct cgaagggcgg ccaggtcggc ggcgtggtgg agaaggaggg
     6841 gccgatccac atctccaacg tgatgccgct cgacccgggc aacggcgagg ccacccgcgt
     6901 cagccacacg atcgaggagg acggccgccg cgtgcgcgta ggcaagcgat ccgggacggc
     6961 gttcgagtga tggccacgac cgaggcgaac atcgtcccgc gcttcaagac gcagtacgag
     7021 caggagatca agcccgctct gatcgagcgc ttcggctact cgagctcgat gcagacgccg
     7081 cgcctcctga agatcacgct gaacatgggc gtcggcgacg ccaagcagga ctcgaacatg
     7141 ctcgaggccg cgcagacgca actcggcatc atcgccgggc agaagcccaa catccgccgc
     7201 gcacgcaagt cgatcgccaa cttcaagctg cgtgaggaga tgcccgtcgg cgtctcggtg
     7261 acgctgcgcc acgcgcgcat gtacgagttt ctcgaccggc tgctgtcggt cgcgatcccg
     7321 cgcatccgcg acttccgcgg cctcaacccg cgctcgttcg acggccgcgg caactactcg
     7381 ctcggcgtcc gcgagcagat catcttcccc gagatcgact acgactcgat cgaccacgtg
     7441 cgcgggctgg acgtgaccat cacgaccagt gcgcagaccg acgaggaggc gttcgtactg
     7501 ctccagtcgt tcgggatgcc gttccgccgc gaggggcgcc cgggcggtcc ggacgccgac
     7561 gccgcggcgg ccgccgcgga ggaggagcgc aaggaggagg cgcgcgcgcg tgccgaggcc
     7621 gagcaggcgg cgctcgacca gttgaaggag gagaacccgg aggcgtacga gacgccgacg
     7681 cgcgccgagg agggcgatcc cgcggccggg gaaaccgagg gcggcgaccc gccgggcgcc
     7741 ggttccgaca gagctacgga ggaccagacc tagtggcaaa gacttctcag gtagtccggc
     7801 agcagaggcc ggccaagtac aagacccgcc agtacaaccg ctgccgccgc tgcggccgcc
     7861 cgcgcgcgta cttccgcaag ttcggcctgt gccggatctg cctgcgcgag atggcccacg
     7921 agggctacgt ccccggcctg accaagtcga gctggtagga gttatcgccc catgtcgatg
     7981 accgatccga tcgccgactt cctgacgcgc atccgcaacg gcctgcgcgc cgaccgcgac
     8041 gcggtcgaca tccccgcctc gcgcttcaag gtcgagatcg cgcgcatcct gcgtgagcag
     8101 ggctacatcg aggactactc ggtcgagccg gccgaggtcg gcgaggtcgt gcgcgtgcgg
     8161 ctcaagtaca cccaggaccg cgcgcccgtc atctccggca tccagcgcgt ctcgcgcccc
     8221 ggacggcgca cgtacgtcaa cgcgaccgag atcccgaagg tcctcggcgg catgggcacg
     8281 tcgatcgtgt ccacctccaa gggcgtcatg accggccacg acgcgcggcg ccagggcgtc
     8341 ggcggcgagg tcgtcgccta cgtttggtag cgatcagatg agccgcatcg gaaagcagcc
     8401 cgttcccttc ccctcgaccg tcgacgtgac gatcgagccc gaggtcgtgc gcgtgaacgg
     8461 accgcgcggg gcgctcgagg agcgcgtctc acgcgacctc acgatcgaga agaacgacgg
     8521 cgagatcgtg gtcacgcgct cgaccaaccg cggcgagcac cgcgcgctgc acgggctcac
     8581 ccgatcgctg atcgcgaaca tggtgcaggg cgtcaccgag ggcttcgaga agcgcctcga
     8641 gatccagggc gtcggctacc gcgcggcgct gcgcggcaag gacatcgacc tccaggtcgg
     8701 ctactcgcac ccggtctcga tcaaggcgcc ggacggcatc gagttcgagg tgccggtgcc
     8761 cacccagatc atcgtgcgcg gcaactcgaa gcaggcggtc ggtgagatcg ccgcccagat
     8821 ccgcaaggtg cgcccgcccg agccgtacaa gggcaagggc atccgctacg cgggcgagca
     8881 cgtgatgcgc aaggtgggca agcgcgcatg agcgtcaaga cgaggcagca ggcgcgcctg
     8941 cgccgccgcc gccgcgtccg cgcgaaggtg cgcggctcgg ccgagcgccc gcggctgtcg
     9001 gtgttccgct cgaacaaggg catccaggcc cagctcatcg acgacgacgc cggccgcacg
     9061 ctggccgccg tgaactgggt cgagtccgac ctgcgcgagc tgaagccgga ggagcaggcc
     9121 aagcgcgtcg ggcagctcct tgccgagcgc gcgagcgccg cgggggtcga gacctgcgtg
     9181 ttcgaccgcg gcggctacct ctaccacggg cgcgtccgcg cgctcgccga ggcggccagg
     9241 gaggcgggtc tccgcttcta ggcggagggc ccgactcgct aacctcgctc agtcatggca
     9301 cgcgacggac agagacgcga tcgcgaccgg ggcggccccg gcagcgacct gcaggagcgg
     9361 gtcgtcgaga tcaaccgcgt cgcgaaggtc gtcaagggcg gccgccgctt ctcgttcacg
     9421 gcgctcgtgg tcgtgggcga cgagaagtcc caggtgggcg tcggctacgg caaggccaac
     9481 gaggtcccgc tcgcgatcca gacgggcgtc gagcgcgccc gccgcaacct cttcaaggtg
     9541 ccgaagcacg gcagcacgat cccgcaccag gtcaccggcg tatacggcgc cggccgcgtc
     9601 ctgctgcgcc ccgcctccga gggcaccggc gtcatcgccg gcggcggcgt gcgcgcggtg
     9661 ctggagctag cgggcatcca cgacgtgctg tcgaagtcgc tcgggtcgca gaacccgatc
     9721 aacctcgtca aggccacgat caccgcgctc caggagctgc gctcgccgct cgaggtggcc
     9781 gagctgcgcg gcctgagtgt cgcccaggtg ctcggcgtcg acaacaaggc cgggaacggc
     9841 aacggcgccg cggctgagtc cgactcgccg gagggcgccg cggctgagtc cgaggcgccg
     9901 gaggctcccg agacggagac ggcgccggca tgacgctgaa gctgacgcag gtcaagtccg
     9961 cgaacggcac aaacccgaag cagaagggcg cgctgcgctc gctcgggctc ggccggatcg
    10021 gcaagaccac cgagcggccc gacgatccca ccgtccgcgg cgccgtccag tccgtccgcc
    10081 acctcctgag ggtcgaagag tgagcgagga gatcggtctc cacaacctga agccggcccc
    10141 gggctcgcgc caccggcgca agcgcgtggg acgcggcgag ggctccggcg tcggcaagac
    10201 gtcgggtcgc ggccagaagg gctacggctc gcgcgcgggg agcaagtcgc gccccggcgc
    10261 cgagggcggc cagatgccga ttcacatgcg gatgcgcaag ctgcgcggcc cgcacatgaa
    10321 gaagtcgatg ccgttcgagc cgttccggac gcagacccag ccggtcaacc tccgcgacct
    10381 cgacgcgcgc ttcggcgacg gcgactcggt cacgccccac acgctgaagg tgcatggcct
    10441 cgccacccgc acgggggtgc cggtcaagat cctcggcgtc ggcgagctct ccaagaagct
    10501 caccgtgagc gcgcacggct tctcgaagag cgcccgcgag aagatcgagg gcgccggcgg
    10561 cacctgcacc gtcctggacg actgaccatg gtcgccatgc ccagat
//
LOCUS       DASVPL010000243         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_108225, whole genome shotgun sequence.
ACCESSION   DASVPL010000243 DASVPL010000000
VERSION     DASVPL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108225"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2499
                     /locus_tag="VF517_13105"
     CDS             <1..>2499
                     /locus_tag="VF517_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX8053922.1"
                     /translation="LGRLREGERVLVHAGAGGVGMAAIQIARHGGAEVYATASPAKWD
                     VLRELGLDDDHIASSRDLDFRERFLEATGEEGMDLVLDSLAGEFVDASLDLLPRGGRF
                     VEIGKADVREPETVAEEHSGVEYRAFDLVEAGPERIQEMMVEVVGLLEGGALRHPPVT
                     SWDVHRAPAAFRHMREARHVGKIVLSVPRTPEPDGTILITGGTGGLGALLARHLAERG
                     AGRLVLTSRRGAETPGVDALEAELRELGATPVVAACDVADRDQLEALLGEIPDEHPLT
                     TVIHAAGVLDDGTIGSLTREQLDRVMRPKVDGALNLHELTAGLDLAEFLMFSSLAGTL
                     GSPGQANYAAANAFLDALARSRQADGLPAKSLVWGAWSESAGMAGDRDAADTDRLRRL
                     GAELMTNDEGLALFDAARDAGDAVPVPVRLDTAALRAQARDGLLPPILSGLVRAPSRS
                     SGARTGSLARRLAELPEDKWDEAVLELVRRDVAAVLGHDSPDQVGPQRSFKELGFDSL
                     SAVELRNRLTQTTGLRLTSTLVFDHPTIEAAARFVRSEVSGVERREPARPSAPTRSDE
                     PIAIVGMSCRYPGGASSPDELWELVTSGTDAISSFPDDRGWDVDRLYDPDPDKPHTCY
                     VREGGFLYDATDFDARFFKIGQGEATAMDPQQRLMLETTWDAFQNAGIDPASLRETDT
                     GVFAGAIEMGYRREVPAAYEAFRMTGTTLSVLSGRLAHTFGLQGPAMTVDTACSSSLV
                     ALHLACQSLIRGECSLALAGGVTVMAAPDLFVDFARQRGLAPDGRCKSYGAGADGTGF
                     SEGAGVLVLERLSEARRLGHRVLAVVRGSAVNQD"
BASE COUNT          323 a          904 c          935 g          337 t
ORIGIN      
        1 acctcggccg cctgcgcgag ggcgagaggg tgctcgtgca cgccggcgcg ggcggcgtcg
       61 ggatggccgc gatccagatc gcccggcacg gcggggccga ggtctacgcg acggccagcc
      121 ccgcgaagtg ggacgtcctg cgcgagctgg ggctcgacga cgaccacatc gcctcctcgc
      181 gcgacctcga cttccgcgag cgcttcctgg aggcgaccgg cgaggagggc atggatctgg
      241 tgctcgactc gctggcgggc gagttcgtcg atgcctcgct cgacctgctg ccgcgcggcg
      301 ggcgcttcgt cgagatcggc aaggccgacg tccgtgagcc ggagacggtg gccgaggagc
      361 actccggcgt cgagtaccgg gccttcgacc tggtcgaggc cgggcccgag cgcatccagg
      421 agatgatggt ggaggtcgtc gggctgttgg agggcggcgc cctccgccac ccgccggtca
      481 cgagctggga cgtgcaccgc gcaccggcgg cgttccgcca catgcgcgag gcgcgccacg
      541 tcggcaagat cgtgctgagc gtcccccgga cgccggagcc cgacgggacg atcctcatca
      601 ccggcggcac cggcgggctg ggtgcgctcc tcgcccgcca cctcgccgag cggggcgccg
      661 gtcgcctggt cctgacgagc cggcgcggcg ccgagacgcc gggagtggac gcgctcgagg
      721 ccgagctcag ggagctcggc gccacgccgg tcgttgcggc ctgcgacgta gccgaccgcg
      781 accagctcga ggccctgctc ggggagatcc ccgacgagca tccgctgacg acggtgatcc
      841 acgccgccgg cgtgctcgac gacgggacga tcggctcgct gacgcgcgag cagctcgacc
      901 gcgtgatgcg cccgaaggtc gacggagcgc tcaacctgca cgagctcacc gcggggctcg
      961 acctcgcgga gttcctgatg ttctcctcgc tggcggggac gctcggctcg cccgggcagg
     1021 cgaactacgc ggccgccaac gcgttcctcg acgcgctcgc gcgcagccgg caggccgacg
     1081 ggctcccggc caagtcgctc gtgtgggggg cctggtccga gtccgccggc atggctggcg
     1141 atcgagacgc ggccgacacg gaccgcctgc ggcgcctcgg tgccgagttg atgacgaacg
     1201 acgaggggct cgcgctcttc gacgcggcgc gcgacgccgg cgacgccgtc ccggtccccg
     1261 tgcgcctcga caccgcggct ctgcgcgcgc aggcgcgtga cgggctgctg ccgccgatcc
     1321 tgagcggact cgtgcgcgct ccgtcacggt cgtcgggcgc gcgcaccgga tcgctcgcgc
     1381 ggcggctcgc cgagctgccg gaggacaagt gggacgaggc ggtgctcgag ctcgtccgca
     1441 gggacgtggc cgcggtgctc gggcacgatt cgcccgacca ggtcggcccg cagcgctcgt
     1501 tcaaggagct cggcttcgac tcgctctcgg cggtcgagct gcgcaaccgt ctgacgcaga
     1561 ccaccgggct gcgactgacc tcgacgctcg tgttcgacca cccgacgatc gaggccgccg
     1621 cgcggttcgt gcgctcggag gtctccggcg tcgagcgtcg cgagcccgcg aggccgtccg
     1681 cgcccacgcg cagcgacgag ccgatcgcga tcgtcggcat gagctgcagg tatccgggcg
     1741 gagcttcctc gcccgacgag ctgtgggagc tcgtcaccag cggcaccgac gccatatcga
     1801 gcttccccga cgaccgcggc tgggacgtgg accgcctcta cgacccggac cccgacaagc
     1861 cccacacctg ttacgtgcgc gaaggcggct tcctatacga cgccacggac ttcgacgcga
     1921 ggttcttcaa gatcgggcag ggcgaggcca cggcgatgga cccccagcag cgactgatgc
     1981 tggagacgac gtgggacgct ttccagaacg ccggtatcga tccggcctcg ctgcgcgaga
     2041 cggacaccgg cgtgttcgcg ggcgcgatcg agatgggcta ccgcagggag gtgcccgcgg
     2101 cgtacgaggc cttccggatg accgggacca cgttgagcgt gctgtccggc cggctcgccc
     2161 acacgttcgg cctgcagggg ccggccatga ccgtcgacac ggcgtgctcg tcgtcgctcg
     2221 tggcgttgca cctcgcgtgc cagtcgctga tccgaggcga atgctcactc gcgctggccg
     2281 gcggcgtgac cgtcatggcc gcgcccgacc tgttcgtcga cttcgcgcgc cagcgcggcc
     2341 tcgcgcccga cgggcgctgc aagtcctatg gggcgggtgc cgacggcacg gggttctcgg
     2401 agggcgccgg ggtcctcgtg ctcgagcgcc tctccgaggc gcgccggctc gggcaccgcg
     2461 tcctggccgt ggtgcgcggc tcggcggtca accaggacg
//
LOCUS       DASVPL010000244         7018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_303281, whole genome shotgun sequence.
ACCESSION   DASVPL010000244 DASVPL010000000
VERSION     DASVPL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303281"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..597)
                     /locus_tag="VF517_13110"
     CDS             complement(58..597)
                     /locus_tag="VF517_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8053923.1"
                     /translation="MSAALLKDLRTSRGEETMRSLYRTYGGELYGFALNCLGDRGLAE
                     EVVQDVFTRVWRYADSFDPERASFRTWIYGIARNAIIDLERRRSVRFGLAPREDDGDE
                     IATDDSLEHALLGWQVAAALTRLTPEHRQVIRLAHFQGMRLREIAELTGLPLGTVKSR
                     VSYALRGLRLALEEMGVEP"
     gene            complement(740..810)
                     /locus_tag="VF517_13115"
     tRNA            complement(740..810)
                     /locus_tag="VF517_13115"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:Gln,seq:ctg)
     gene            862..1140
                     /locus_tag="VF517_13120"
     CDS             862..1140
                     /locus_tag="VF517_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX8053924.1"
                     /translation="MSRAIPLEDVLSSHRSTNRWRLKARLIAAGLKDDRCERCGIASW
                     RDRPLSLQLHHVNGVADDNRLENIELLCPNCHSQTPTHSSKRGSRRSE"
     gene            1137..1721
                     /locus_tag="VF517_13125"
     CDS             1137..1721
                     /locus_tag="VF517_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX8053925.1"
                     /translation="MTSSGPRARPGVTRMLVAARLDSGTSVKEVARQLGLSKSTVSWH
                     AARLGRPRQSRSAVRYDWAAVQSYYDEGRSPRECCERFGFNRSAWAAAVKSGRVVVRP
                     RRAPLCDLLVADSPASRLTVKRRLLREGIKSSRCDECGLERWQGRQLRFELHHINGNR
                     SDHRLMNLRLLCPNCHSQTENFAGRALPSRRKES"
     gene            complement(1718..2947)
                     /locus_tag="VF517_13130"
     CDS             complement(1718..2947)
                     /locus_tag="VF517_13130"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8053926.1"
                     /translation="MADRSVDYLLIGGGIACARCAQTLRREGAEGSILVVGREADPAY
                     ARPPLSKEYLAGTMPREEVTSVEPEWWEENGVELLTRTSVMKLDTGERVAKLATREEV
                     GFDKALVATGANVRRLRADGGELKGIHYLRSYGNADAIRADAEAGKRAVMIGGSYIGS
                     EVAATLTTAFGVECTILMQEDVCLSPQFGNEVGGYFQGLLESHGITAHGGDSLERYEG
                     EERVARVVSQRGLELDCDFVVVGAGVVPDVMLARSAGLELGESGGIACSSRLETSVPG
                     IYAAGDVAEYDSPLHGRPVRIEHWDVAAEHGKTAARNMLGQDVAHEVVPYFWSDFADW
                     AKLEYVGIGSGERRVIRGSLDDGDFIAFSLDGDRLVAAVTVGRPEDLAEARRLMADRA
                     TPDPDALADVSTDLSSL"
     gene            complement(2947..3624)
                     /locus_tag="VF517_13135"
     CDS             complement(2947..3624)
                     /locus_tag="VF517_13135"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX8053927.1"
                     /translation="MYILIVGAGKVGWNLARELIDRGNEVTVVESDRNRYATVEEELE
                     HAAHSGDGSELWVLERAGIERAEMVIAVTGDDEDNILISQVAREKYGVERVIARCNNP
                     RNLQHFDLLGVRPTVSATDLILRLIEHEVPRYGPLHLLDLPQERLEIIEVEVSAGSAA
                     DGAEVKDIGLPEGSLVISILRDGTGFVPLSDSVIEAGDEVLVVLDTGLEEKVSAPFRA
                     RQESPAG"
     gene            complement(3624..4046)
                     /locus_tag="VF517_13140"
     CDS             complement(3624..4046)
                     /locus_tag="VF517_13140"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX8053928.1"
                     /translation="MFVLIVGCGRVGSTVAKRMLAGGHEVSVLDEDPEAVAQLERGED
                     RTWEEMGGSFTVGTALEIDALLEAGIERADAFIASTDGDNTNLVIAQIAQRRFEIERV
                     VVRVLDPARASWYEEQGLRTVSPTKVAIDMLTEAVGVG"
     gene            complement(4085..6064)
                     /locus_tag="VF517_13145"
     CDS             complement(4085..6064)
                     /locus_tag="VF517_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8053929.1"
                     /translation="MNRSRVTPAALPAQARLRVSRGLGQTALAAIGLSAIGASIYFIL
                     GIVAGRALGLTPFVFGLTGVFFVITAMTYVEANSIHPERGGASTFARYAFDELWSFVA
                     GWAILLDYLIVMAIGSFAVSHYLAAFWGELAEPPIELIATTLVIGFVTWSNIRGSSAS
                     RFGRLARVGLANLALMAVIIAIGLITEYKPGEVVESIDLGVAPEWHDLWFAAVLAAVA
                     FLGIEAASGLAGEVRVGGGGLRRLIGFAVLVVVVALVGMSAVGLMVLPVENGQTELGT
                     RYVEAPVLGIVSGFDPAWLRDALRYSVGVMGAVVLVQAVNGTMLGLSRLTYSLATHRQ
                     IPSVIGRLDASRGTPYVAISLAAAIALGLAFSSDIEFLAGIFAFGAMLAFTLAHASVI
                     VMRFREREAPRAFRIPLSVPVRGGSLPLPAVVGAVGSFAAWVSVIVLHEGARAVGGIW
                     MAAGLVLYVTYRLGQDKSLRKRYAIPQEALQDRRGSPAAEYGSILVPVFGSALDDDIV
                     GTAGRLAAEEGEEGEGGAVIEALYVVEVPMSLPLDARVPDEKIVEAMRAVARAKEVGE
                     EYEGVVVATAMVRARAAGAAIVSEARRRGVEAIVLAAEAPTGTRGGKLLGGRADPRDR
                     AVGETTRYVLEKAPCRVILTASPPGEDGYLEGVAP"
     gene            complement(6061..6261)
                     /locus_tag="VF517_13150"
     CDS             complement(6061..6261)
                     /locus_tag="VF517_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053930.1"
                     /translation="MERAYRHTTMAFGLAICVLGVTMTVLALVRGAGPLALGVLVGVA
                     FTVLGAGRLYLATRPDATHGRR"
     gene            complement(6272..>7018)
                     /locus_tag="VF517_13155"
     CDS             complement(6272..>7018)
                     /locus_tag="VF517_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8053931.1"
                     /translation="FTIAHLSLVALRVKVPDQERPYTAPGNVTIRGQRLPLFAMFGAV
                     GTGLAFVVVTILNVTTLIAGSIWLTIGVASYVAYRRRLGLSLTQTTKIVVPDPIVERE
                     VEYESILVAFEDGVYSADAVATAVKLAARRRRGIHVVATISVPASAPIDAPLPDQEAR
                     ALAAIDSARVLGGRRVTGHWEKVRQGEAGRRIVDEARSMRARAIVMPLMPSRRGGAPV
                     MGRTHETVLEERPCRVIIEATPRERVRAAT"
BASE COUNT         1071 a         2632 c         2370 g          945 t
ORIGIN      
        1 gcggctcgag cgcgtggagc acgtagccgc cgatcagcgg gcggacgtcg gagcacttca
       61 cggctccacc cccatctcct cgagcgccag ccgcaggccg cgcagggcgt acgagacgcg
      121 gctcttcacg gtgccgagcg gcagcccggt gagctcggcg atctcccgca gccgcatgcc
      181 ctggaagtgc gcgagccgga tgacctgccg gtgctcgggg gtgagccgcg tgagcgccgc
      241 ggcgacctgc cagccgagca gcgcgtgctc cagcgagtcg tcggtcgcta tctcgtcgcc
      301 gtcgtcctcg cgcggcgcga gcccgaaccg gaccgagcgg cgccgctcga ggtcgatgat
      361 cgcgttgcgc gcgatcccgt agatccaggt gcgaaagctc gcccgctcgg ggtcgaagct
      421 gtcggcgtag cgccagacgc gtgtgaacac gtcctgcacg acctcctccg cgaggccccg
      481 atcgccgagg cagttcagcg cgaacccgta cagctcgccg ccgtaggtcc ggtagagcga
      541 ccgcatcgtc tcctcgccgc gcgaggtgcg cagatccttg agcagggcgg cggacatggg
      601 ctcagtgtgg tctacgcaga gcgcgctcgc gcggttcggc gcccgaaggc tcggcgcccg
      661 aaggccccct cgcacggttc cgtccgattc gatcccgata ctacgaacaa gcgttcgtat
      721 gacgctttcg acaattgaac tgccgggcag ggattcgaac cccagcttcc agggccagaa
      781 cctggcgtgc tgccgctaca ccacccggca tagaggaggg cgagtatagg tggtgcctgg
      841 agtgagattc gagggctcgt cgtgagccgc gcgattccgc tcgaagatgt tctgtcgtcc
      901 catcggtcga cgaatcggtg gcgactcaag gcacggctga tagccgcggg actgaaggac
      961 gaccgctgcg agcgatgcgg gatcgcgtcg tggcgcgacc ggccactgtc gctgcagctt
     1021 caccacgtca acggcgtcgc ggatgacaac cggctcgaga acatcgagct gctctgtccc
     1081 aactgccata gccagacgcc gactcactcg tcgaagcgtg ggtcgcgccg gagcgaatga
     1141 cgagctcggg accgcgggcc cggcccggcg tcacccgcat gctggtggca gctcgcctgg
     1201 acagcggcac atccgtcaaa gaggtggccc ggcagctcgg cctctcgaag tcgaccgtct
     1261 cgtggcatgc agcccggctc gggaggcccc gccagtcgcg cagcgccgtg cgctacgact
     1321 gggccgcggt gcagtcctac tacgacgaag gccgctctcc aagggagtgc tgcgagcggt
     1381 tcggcttcaa ccgcagcgcc tgggcggccg ctgtgaagag tggccgtgta gtcgtccgcc
     1441 cgcggagggc accgctctgc gacctgctcg tggccgacag tcccgcatcg cgactgacgg
     1501 tcaagaggcg cctgctccga gagggcatca agtcgtcccg gtgcgacgag tgcggcctgg
     1561 agcgatggca gggccggcaa cttcgtttcg agcttcatca catcaacggc aatcgctccg
     1621 atcaccggct catgaacctg cgccttctct gtccgaactg ccacagccag accgagaact
     1681 tcgccgggcg ggcattgccg tcgcggcgga aggagagcta gagcgagctc agatccgtgg
     1741 acacatccgc cagcgcgtcc gggtccggcg tcgcgcggtc ggccatcagg cggcgcgcct
     1801 cggccaggtc ctcggggcgg ccgacggtca ccgcggccac caggcggtcg ccgtcgaggg
     1861 agaaggcgat gaagtcgccg tcgtcgagcg atccccggat cacccgccgc tcgccgctgc
     1921 cgatgcccac gtactcgagc ttcgcccagt cggcgaagtc cgaccagaag tacgggacga
     1981 cctcgtgcgc gacgtcctgg ccgagcatgt tgcgggcggc ggtcttgccg tgctccgcgg
     2041 ccacgtccca atgctcgatg cgcacggggc ggccgtggag cggcgagtcg tactcggcca
     2101 cgtcgccggc ggcgtagatc ccggggaccg aggtctcgag ccgcgacgag cacgcgatcc
     2161 ccccgctctc gcccagctcg aggccggccg accgggcgag catcacgtcc ggaaccacgc
     2221 cggcgcccac gaccacgaag tcgcagtcga gctcgaggcc gcgctggctc accacgcgcg
     2281 cgacgcgctc ctcgccctcg tagcgctcga gcgagtcgcc gccgtgcgcg gtgatcccgt
     2341 gcgactcgag cagcccctgg aagtagccgc ccacctcgtt gccgaactga ggcgagaggc
     2401 acacgtcctc ctgcatcagg atcgtgcact cgacgccgaa cgccgtcgtc agggtggccg
     2461 ccacctccga cccgatgtag ctgccgccga tcatcacggc gcgcttgccc gcctcggcgt
     2521 cggcgcggat cgcgtccgcg ttgccgtacg aacgcaggta gtggatcccc ttgagctcgc
     2581 cgccgtcggc gcgcaatcgt ctgacgttcg ccccggtggc cacgagcgcc ttgtcgaacc
     2641 cgacctcctc gcgggtcgcg agcttcgcca cgcgctcgcc ggtgtcgagc ttcatcacgc
     2701 tcgtccgcgt caggagctcg acgccgttct cctcccacca ctccggctcg accgaggtga
     2761 cctcctcgcg cggcatcgtg ccggccaggt actccttcga cagcggcggg cgcgcgtagg
     2821 ccggatcggc ctcacgcccg acgaccagga tcgatccctc ggcaccctcg cggcggagcg
     2881 tctgcgcgca gcgggcgcat gcgatgccgc cgccgatcag cagatagtcg acggagcggt
     2941 cggccatcag ccggccggcg attcctgccg ggcccggaac ggagcgctga ccttctcctc
     3001 gagaccggtg tcgagcacca ccagcacctc gtccccggcc tcgatgaccg agtccgacag
     3061 cggcacgaag ccggtcccgt cgcgcagtat cgagatcacg agcgagccct ccggaaggcc
     3121 gatgtccttc acctcggcgc cgtccgccgc cgagcccgcc gagacctcca cctcgatgat
     3181 ctcgagccgc tcctgcggca ggtcgagcag gtgcagcggc ccgtagcgcg gcacctcgtg
     3241 ctcgatgagc ctcaggatca ggtcggtcgc ggagacggtc gggcgcacgc cgagcaggtc
     3301 gaagtgctgg aggttgcgcg ggttgttgca gcgcgcgatc acgcgctcca cgccgtactt
     3361 ctcgcgagcg acctgggaga tcaggatgtt gtcctcgtcg tcgccggtca ccgcgatcac
     3421 catctccgcg cgctcgatcc ccgcccgctc gaggacccac agctccgagc cgtcaccgga
     3481 gtgcgccgcg tgctcgagct cctcctcgac ggtcgcatag cggttgcggt cggactcgac
     3541 cacggtcacc tcgttgccgc ggtcgatcag ctcgcgcgcc aggttccacc cgaccttccc
     3601 ggcgccgacg atcaggatgt acatcagccg accccgaccg cttcggtgag catgtcgatg
     3661 gccaccttgg tcggggagac cgtgcgcagc ccctgctcct cgtaccagct cgcgcgcgcc
     3721 gggtcgagca cgcgcaccac cacgcgctcg atctcgaagc ggcgctgtgc gatctgggcg
     3781 atcacgaggt tcgtgttgtc gccgtcggtc gaggcgatga acgcgtccgc gcgctcgatg
     3841 ccggcctcga gcagcgcgtc gatctcgagc gccgtcccga cggtgaacga gccgcccatc
     3901 tcctcccagg tgcggtcctc gccgcgctcg agctgcgcga cggcctcggg gtcctcgtcg
     3961 agcaccgaca cctcgtgccc gccggcgagc atccgcttcg cgacggtcga gcccacgcgg
     4021 ccgcagccca cgatcaatac gaacatgagg cgcaatctac ccgctagggg ccagtcggaa
     4081 gcgtctacgg tgcgacaccc tccaggtagc cgtcctcgcc cggcggggaa gccgtgagga
     4141 tcacgcggca aggggccttc tcgagcacgt agcgcgtggt ctccccaacc gcgcggtcgc
     4201 gtgggtcggc gcggccgccg agcagcttgc cgccgcgtgt gcctgttggc gcctcggcgg
     4261 ccagcacgat cgcctcgacg ccgcgccgcc gcgcctccga gacgatcgcg gcgcccgccg
     4321 cgcgtgcgcg caccatcgcc gtagcgacca ccaccccctc gtactcctcg ccgacctcct
     4381 tcgcgcgtgc gaccgcgcgc attgcctcga cgatcttctc gtcgggcacg cgcgcgtcga
     4441 gcgggagcga catcggcacc tcgaccacgt agagcgcctc gatcaccgcg ccgccctcgc
     4501 cctcctcccc ctcctcggcg gcgagccggc ccgccgtgcc gacgatgtcg tcgtcgagcg
     4561 ccgacccgaa gactgggacg aggatgctcc cgtactccgc cgccggcgac ccgcgcctgt
     4621 cctggagcgc ctcctgcggg atcgcgtagc gcttgcgcag cgacttgtcc tgcccgagcc
     4681 ggtaggtcac gtagagcacg agcccggcgg ccatccagat cccgccgacc gcgcgcgccc
     4741 cctcgtgcag cacgatcacg ctcacccacg cggcgaacga gccgaccgcc ccgaccacgg
     4801 ccggcagcgg cagcgacccg ccgcgcaccg gcaccgagag cgggatacgg aaggcccgcg
     4861 gcgcctcgcg ctcgcggaag cgcatcacga tcaccgacgc gtgcgcgagc gtgaacgcca
     4921 gcatcgcgcc gaacgcgaat atccccgcga ggaactcgat gtcggagctg aacgccagcc
     4981 cgagcgcgat cgccgccgcc agcgagatcg cgacgtaggg cgttccgcgc gaggcgtcga
     5041 ggcgcccgat cacactcggg atctgccggt gggttgcgag cgagtaggtc aggcgggaga
     5101 gaccgagcat cgtgccgttg accgcctgca ccagcacgac cgcgcccatc actccgacgc
     5161 tgtagcgcag cgcgtcgcgc agccaggcgg ggtcgaagcc ggacacgatg ccgagcacgg
     5221 gcgcttcgac gtagcgcgtg ccgagctcgg tctgcccgtt ctccacgggc agcaccatca
     5281 gccccaccgc cgacatgccg acgagcgcga cgaccaccac gaggaccgcg aagccgatca
     5341 gccggcgcag cccgccgccg ccgacgcgaa cctcgcccgc gagccccgag gccgcctcga
     5401 tcccgaggaa cgccacggcc gccagcaccg ccgcgaacca gaggtcgtgc cactccggcg
     5461 ccacgccgag gtcgatcgac tccacgacct cgcccggctt gtactcggtg atcaggccga
     5521 tcgcgatgat cacggccatc agcgcgaggt tcgccaggcc gacccgcgcg agccggccga
     5581 accggctggc ggatgagccg cggatgttcg accaggtgac gaacccgatc acgagcgtgg
     5641 tcgcgatcag ctcgatcggc ggctcggcga gctcgcccca gaacgccgcg aggtagtgcg
     5701 acacggcgaa cgagccgatc gccatcacga tcaggtagtc gaggaggatc gcccagcccg
     5761 cgacgaagct ccagagctcg tcgaacgcgt agcgggcgaa cgtggaggcc ccgccgcgct
     5821 cggggtggat cgagttggcc tccacgtacg tcatcgcggt gatgacgaag aagaccccgg
     5881 tgaggccgaa cacgaacggc gtgagcccca gcgcccggcc ggcgacgatt ccgaggatga
     5941 aatagatcga cgcgccgatc gcgctcagcc cgatcgccgc gagcgcggtc tggcccagcc
     6001 cgcgcgagac ccgcagccgc gcctgcgctg gcagcgcggc gggcgtgacg cgggacctgt
     6061 tcaccgccgg ccgtgcgttg cgtcgggccg cgtggcgagg tagaggcgcc ccgcaccgag
     6121 cacggtgaac gccaccccga ccaggacccc gagcgccagc gggccggcgc cccgcacgag
     6181 cgccaggacg gtcatcgtca cgccgagcac gcagatcgcc aggccgaacg ccatggtcgt
     6241 atggcgatag gcccgctcca ccggcgcgat tctacgtcgc ggcgcgcacc cgctcgcgcg
     6301 gggtcgcctc gatgatcacc cggcacgggc gctcctcgag cacggtctcg tgggtgcgcc
     6361 ccatgaccgg cgcaccgccg cggcgcgacg gcatcagcgg catcacgatc gcccgcgcgc
     6421 gcatcgaccg cgcctcgtcc acgatccgcc ggccggcctc gccctggcgc accttctccc
     6481 aatgcccggt cacgcgccgt cccccgagca cgcgcgccga gtcgatcgcg gccagggcgc
     6541 gcgcctcctg gtcgggcagc ggcgcgtcga tcggggcgct ggcgggcacg gagatcgtgg
     6601 ccaccacgtg aatgccgcgc cgtcgccgcg cggcgagctt caccgccgtc gcgaccgcgt
     6661 ccgcggagta gaccccgtcc tcgaacgcaa ccaggatcga ctcgtactcg acctcgcgct
     6721 cgacgatcgg gtcggggacc acgatcttcg tcgtctgcgt gaggctgagc ccgagccgcc
     6781 tgcggtaggc gacgtagctc gccaccccga tcgtcagcca gatagaaccg gcgatcagcg
     6841 tcgtgacgtt caggatcgtg accacgacga acgcgagccc ggtccccacc gcgccgaaca
     6901 tcgcgaacag cggcaggcgc tgcccgcgga tcgtcacgtt gccgggcgcg gtgtacgggc
     6961 gctcctggtc gggcaccttc acgcgcagcg cgacgagcga caggtgcgcg atcgtgaa
//
LOCUS       DASVPL010000245        37235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_185054, whole genome shotgun sequence.
ACCESSION   DASVPL010000245 DASVPL010000000
VERSION     DASVPL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 37235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37235
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185054"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VF517_13160"
     CDS             complement(<1..361)
                     /locus_tag="VF517_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053932.1"
                     /translation="MSSWPEQDLRERVAAGGGRVSISAEDLLGGFGLRRATPTARRNV
                     EAWLAEAGLAVEPSLGTNGLPETVTLYDPAAPAEPAEPAATEPVAAPPPAAADRVPSP
                     EPAPAELAATPAAAAPAP"
     gene            complement(479..799)
                     /locus_tag="VF517_13165"
     CDS             complement(479..799)
                     /locus_tag="VF517_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX8053933.1"
                     /translation="MTLVRRRFRGMIALVALVTAVALVVTAAPVMAQSTSAVTQADCD
                     AGRIKDKQGNTLKGERCERLIGQRVRLAQTGFEAWYLLLGGVACIGGAAALRMRRRPT
                     PQLG"
     gene            complement(900..1799)
                     /locus_tag="VF517_13170"
     CDS             complement(900..1799)
                     /locus_tag="VF517_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8053934.1"
                     /translation="MAVALLTAAAAVPAHAQEGPRAPDAPPLFRPAARAPLPDPPKFV
                     DVNGGAPTARDQAKPLFDPSTRAAAAYRHPLAKRVAAATAYARARSGRVAFAVIDLDG
                     RLRGWHVDEQHRSASVVKAMFLVAYLNRIRGRALSTSDRRLLGPMIVRSDNDRASRVY
                     GILGPGAVYALARRARMTRFVLKARWGDTLITAADQARYFARIDLLVPERHRAYARKL
                     LASIIEEQRWGVPPAIPSGWRVFFKGGWRPLGRRNLVNQSALIESGDRRVALAVLTDG
                     NPSHVYGTETIRGVARRVLRGLR"
     gene            complement(1850..3115)
                     /locus_tag="VF517_13175"
     CDS             complement(1850..3115)
                     /locus_tag="VF517_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX8053935.1"
                     /translation="MACATLVALAGAPAAQAGKRMEVAMSDDPVLLARHYYDRDPALQ
                     NARELGVTRLRVFVNWAGVMGKQREAVDPPQQPTYYWGIYEELIDAAARAGMRVQLDL
                     AGPAPRFATGDRELSMTRPDPAQFAAFARAAATRFRGRVDRYSIWNEPNHDGWLRPSA
                     EAPQLYRALYVAGYAAIKQADPGAAVLFGETAPYHGTRAMAPLTFLRRTLCLTPKYKL
                     DKPCLAKLPVGLRGRLATDGYAHHPYDFRNPPEYKYRGLDNVTMGTLSRLTTALDKAA
                     RARALWGPKKRRAPDVFLSEFGYFATGPRAKLPEKKRAAYLPRAFTIAQKNKRVRQML
                     QYGLVAPPSGYPGAAFDFGLVTLDGSPRATYGALVQWAAKALAKKQIAAPGGPIALPA
                     AQPGADPQPEAPPEEEPEEQPPFPLPPFP"
     gene            3189..4436
                     /locus_tag="VF517_13180"
     CDS             3189..4436
                     /locus_tag="VF517_13180"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8053936.1"
                     /translation="MIEVEHTTVETGLPLVRLSVPGTRAVTALVAFDAGARTERPEEN
                     GMAHFLEHLVFKGGESFPTYRDVNETAERLGAQLNAYTSHDIVAFHITCRSEAVMEAL
                     DLLTDFVGRARIDADELDRERGVVIQEIARSNDAPASVADRLIDAATYGEHPLGRSVL
                     GPEEHLRTFTRDAIVAFRERQWASARGGAFLAGDLTHLDADGAAELFTRFPARNGTPE
                     ADPAPSFERRVRVERRDTQQSHLRLHWRPAADPTDLRQRAALSVYSTLLGGSMGSRLF
                     DEIREQRGLCYAIRSYGTSHADVSRLDVSSGLDSTKCPEAYERIREIIADLAENGPRD
                     GEVERARSYTAGSTVLALESTRVVASRAASQKVTFGEISSPDEMIAALDAVTENDVRE
                     VAAQVTGEPVVACVGPHEAADFE"
     gene            complement(4440..5597)
                     /locus_tag="VF517_13185"
     CDS             complement(4440..5597)
                     /locus_tag="VF517_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX8053937.1"
                     /translation="MGPAELAARAREHGRIAIDTEFVSEGRYRPLLCLVQVAVAPRDN
                     NGEPHIEVLDPIEGFDHEPLAAVLADPEVEVVIHAGGQDVPILRRAWGVGPTNVFDTQ
                     VAAGFAGFSAQAGYGNLLQKVAGVQLNKSAGFTRWDRRPLTDEQIEYAKGDVEDLLAL
                     SDTLQERLAKAGRLDWAREECRRLEGIDDQRDADEAYRRLPRVSRLRPKQRAVAYALA
                     DWRERTAATEDRPVGSVLSDVALVEIARRQPESIDALTAIRGVRPDLGRRRGNAVLEV
                     IRTGRDREPPPAEEFTRTDPDPGAAARIALCESVVRQRVGEAGLAYELVASRADLTEI
                     VVAARAGSDEPDVRTLRGWRRDLVGDELLELLAGRRVVRATRRGVEIEPAD"
     gene            5642..6253
                     /locus_tag="VF517_13190"
     CDS             5642..6253
                     /locus_tag="VF517_13190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8053938.1"
                     /translation="MRRVVAAARRAGLLVRLLPPRVAWFYFRARRTAARSGDDWSLKS
                     ATKPESLALLLKLARGRRAVVEIGTGTAWTTAALALADPRRNVVSYDPVMRPERDRYL
                     AIAGPSATARIELIAAGGEAGPAPEGPAPDFVFVDGSHERELTISTFEAWRGALAPGG
                     VIAFHDYGNELYPGVSEAIAELGIEGRVERDVFVWSGSLPSEG"
     gene            6250..6858
                     /locus_tag="VF517_13195"
     CDS             6250..6858
                     /locus_tag="VF517_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX8053939.1"
                     /translation="MIVTITPHAVEKVREFRANVEDPDAQAMWVRVTGVHRGEFTYDL
                     ALRPLSAAGPGDEVEHVDGLAVVVAGEDVDSLRGATIDWSDDLMRGGLTVSNPNRPQV
                     VTPALGNGELSGDVEDRVAQVIDRRINPAIASHGGRATLDHVVDGIAYVRLGGGCQGC
                     GMATVTLDQGIESAILESVPEIRQVVDVTDHAAGENPFYQPA"
     gene            complement(6872..9655)
                     /gene="fxsT"
                     /locus_tag="VF517_13200"
     CDS             complement(6872..9655)
                     /gene="fxsT"
                     /locus_tag="VF517_13200"
                     /inference="COORDINATES: protein motif:HMM:NF040586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxSxx-COOH system tetratricopeptide repeat
                     protein"
                     /protein_id="HEX8053940.1"
                     /translation="MGGALPGAGFFLAPGLVVTCAHVTGDVEELEVLWRPDAAPVPAR
                     LELKLCDRGRPVPDLAADYPDIALLRVEAAGHPCVALDEDMPTYGDSFQSYGYPEEGG
                     SVLLTPAALDYRGTKSQHPTPFIDLASDTVKPGMSGGPLLNVRSKAVCGVLVATRSPS
                     SPDGGLAVPWVAVRDQLAEAMGANRAFHAANHSWSDVARPAGRRVRFRLPHVVKHFSG
                     RGPELETLERALADDSRAIVTQAITGLGGVGKSQLAARYVQKHVDDYDVVAWIRAEDG
                     GIADLADLALELGEEVEGLAPADRAQVAVRVLENAGARWLLVLDNVATPEQLAVCCPA
                     TGNGNVLVTSRNRAVGEFGALLDVDVFEDEVGADFLVGRAGRDGEREAALRLTRALGG
                     LPLALAHAGAYCSSGTSFDEYLKMLTELPSAEVFDESPEAFYSQTVASTWQVSIDAAA
                     ERASLAPAVLGMASYLAPDKIPLPLFRVLLGDPNNIRERKRLSDAFNALHGLSLVEVG
                     EASLSVHRLLQKTVRDQARLGGDEEGPAFAARAVLVAFPKDRRVPSEWPRCEELLPHA
                     AAMAHTIEPRPERTDVAEVLRRACGYLLEVRDVRRGVPMAESALEYSQHALGPAHERT
                     LAALANLANAVWAAGRTKDALDLEERVLEDYERVFGSDHPSTLMVRANLAASYHSAGR
                     TEAAIAAFERVIAARKRLLGDDHEDTLTARAGLAGAYMAASRADDAIALYEAVIATRE
                     RTLGAEYPTTLTARISLGSALTEAGRSADAVRLMEGTLEVASAILGPNHPDTLAARSI
                     LGGAYDAEGRAGEAIALRSAALSCAEEILGRLHPDVLIMKNNLAASLERAHRIDEAIA
                     LRESVIAGAESTLGAAHPHSLTYRANLGVTFAKAGRHADAVELLTDVAAEMERVLGPE
                     DANTLALRAFLQS"
     gene            complement(9694..10008)
                     /locus_tag="VF517_13205"
     CDS             complement(9694..10008)
                     /locus_tag="VF517_13205"
                     /inference="COORDINATES: protein motif:HMM:NF041216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CU044_2847 family protein"
                     /protein_id="HEX8053941.1"
                     /translation="MNGDTVVDAALPGGGVVQVRVAEGASGIGSVGRNELELEEALEP
                     IGEVAAIVRDKLAALGATKATAAFGVSLTVKTGKLSALIFESQGAASLTVTLEWVNPA
                     PA"
     gene            complement(10024..10911)
                     /locus_tag="VF517_13210"
     CDS             complement(10024..10911)
                     /locus_tag="VF517_13210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8053942.1"
                     /translation="MSSRAWAVFAAMSVLWGIPYLFIKVAVDDGLSPWFLSWARLVLG
                     AAVLLTLAWRAGKLVELRGHWTWIVLFAVVEISLPFPLIAAGEQHVASSLAAILIAAV
                     PLFVALLALRFDAEERVGGMRLVGLVVGLAGVVALVGIDVAGRSDELLGALAILLAAL
                     GYAAGPMVLNRKLGEVDARATMGASLAVAALVLTLPAALTWPADAPTGDALVSIVVLG
                     LFCTALAFVLWSVLIAEAGPGRAAVITYVAPAVAIALGVAVLDEEVGLGAVVGLVLIL
                     AGSWLATGGRLPRRSVKLA"
     gene            complement(10908..12803)
                     /locus_tag="VF517_13215"
     CDS             complement(10908..12803)
                     /locus_tag="VF517_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8053943.1"
                     /translation="MQNQALHARSSFPPIGDYGFLSDGEVAALVAPSGNVEWLCVPQF
                     DSPAVFATILDRDAGRFRLGPTDAQVPAARRYLPGGVVLETTWKTRTGWLMVRDALTV
                     GPWHHVEERAHRQHRPPSDWEADHVLVRTARCAQGIVELQLECEPVFGYGERAAEWEY
                     TGSGYTEAVARGGKDDFELGLDTDMRVGFEGSRATARTTMRKGDVAYVALSWSEHGGP
                     KSYHDAFGMMDQTNTYWREWLARGEFPDHPWRAQLERSALTLRGLTYAPTGAIVSAAT
                     TSLPRVSGGERNYDYRYSFMRDAAFALWGSYSLGFDWEADDFFYFLAELAEDGQELQN
                     IVAIDGRRDLSEHTVDQLSGYEGSRPVRVGNAAHRSTQHDVWGAMADAAYLHTRSRDK
                     MPERIWPIVNELVTRAVSDWREPDRGIWATRGEPRHYTSSKVMCWVAADRGARLAELR
                     QEDGLAEEWQGCADAIREDVIANALSDRGVFAQHYDTEALDASLLLMPLVRFLPGDDE
                     RVRRTVEAIADELVVDGLVMRQRPEEEDFAVEGETFTVCSFWLVSAFVEIGEVERARE
                     LCERLLALASPLGLYAEHIDPDTGRHLGNFPHAFTHLAQVNAALHVIRAEGEPVADTR
                     VDRPTTA"
     gene            complement(12833..13417)
                     /locus_tag="VF517_13220"
     CDS             complement(12833..13417)
                     /locus_tag="VF517_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053944.1"
                     /translation="MARRIALLALALLALAPATAPAATTNPADALAATAIDPYRYDHA
                     KSCRKSPPPGTLALKAWLEANAGGAFWGTMRCERWGKDSASLHAEGRAIDWHLNVHRS
                     ADKREASRLIRLFLATDRVGNRHALARRMGIQELIWNCRSWWSGGDGMRPYSACYDSA
                     GRPKKIDDTTAHRDHIHIGLNWPGARKQTSFWLR"
     gene            complement(13465..14595)
                     /locus_tag="VF517_13225"
     CDS             complement(13465..14595)
                     /locus_tag="VF517_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8053945.1"
                     /translation="MHPWVESHASRMALRAEREMEALVAVSSPSGDVHGAEEAVAVCA
                     ALLPPEAEVERIPCSTAGYADDLLAHVRGTGKRRVLLLGHLDTVVAHESHRPIERRGD
                     RIIGSGTVDMKGGVALSLALFEVLAERPEAYAEIALLLVNDEEWRVGSLHHVHRFEGF
                     DACLCFEAGQHGPEGEEGLIVRRKAASTLKVVAHGLSSHSGSAPDRGRNALLALAAAA
                     QAVAAHHDPQGPDRLSAVPTILTSGDAFNVVPSGGELFCDMRADDAAAFHRVLAEVPD
                     EVGGARLEAEIVRLWPGMDSRETTRDLLAATAEVLGRPVIPVERGGASDASHVSSAVP
                     LTLDGFGPRGGQAHNPDEFVLAESIHQRAEVALALMAALLEL"
     gene            complement(14622..15881)
                     /locus_tag="VF517_13230"
     CDS             complement(14622..15881)
                     /locus_tag="VF517_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX8053946.1"
                     /translation="MPDMLELADRLWSGEADLAEHHPVGFLGELTEVREGIAFLPSFA
                     NVTAVETDEGLVLFDSGSFALAQWAFESIRGWRPDTCVHTVVFTHGHVDHVFGLPLWE
                     EEAVDRGWIPPIVVAHENMPARFDRYRLTAGYNAAINRRQFGVDELEWPLDYRYPDKT
                     YRDELELEIGGERFELHHAKGETDDYTWTWIPGRRTICCGDLFIWAVPNAGNPQKVQR
                     YPREWVQALRAMAELDAELLLPGHGLPVVGAERVRAALEDSAALLESLVEQTLALMNE
                     GAPLDQILHAVRPPANLLEKPYLRPIYDEPEFIVRNLWRQFGGWYDGNPANLKPAPDA
                     ALAGELAGLTGGGERLAERALELAEAGELRLAGHLAELAALAEPELEAAQRARAEVNE
                     CRAQDASSTMARGVFNAAARESRARLG"
     gene            15920..17356
                     /locus_tag="VF517_13235"
     CDS             15920..17356
                     /locus_tag="VF517_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8053947.1"
                     /translation="MSRQAPMLQALDDAPITKRYVILAVVTMFGAVLDLFDFFLIAFI
                     VPVIDDEWQLTFGQATAVLLSAGVGAIAGALIWGWLGDRYGRRKPLIAGILTFSVATG
                     LLALAPDDGWWFLALGRIVVGAGVAGVAVIAVPLTLEFTPTRMRTTIVGFVTTATVPI
                     GILAAALAAALLADPLGWRPLFAVGVVPAVLALFIKWYVPESPRWLIDQGRPEEARRS
                     IAYLLMEPEESLSLEVPPRDREPEIGFRQLFRYRNSLLVTCTAWFGASVAVSGLILWG
                     PTFLKEILEIDSDEAALLFVFVTLGSFAGRLFFSFAPARFGRRACGVVTGLGAAPLLF
                     LAAASGETEVLGISLFLVALVLASFFVDGGFANLAPLTPELFPTSLRTQGLGLSWAFS
                     GLGRIVGPLVVGAWAAEGNDPIEPEATLDALLPAFSFLAVAALAAGLVFLVLRTEPHG
                     RDLESLSDELRESVAAPGAAAAEPAAPA"
     gene            complement(17600..19078)
                     /locus_tag="VF517_13240"
     CDS             complement(17600..19078)
                     /locus_tag="VF517_13240"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053948.1"
                     /translation="MNKQAPSPTKIFVMVMWALSCFGLLLWLWFSFGGPVPLRPEGYR
                     VKVKMPESALLVKEADIRMAGLDIGKVKNLSLAREGGNVVEMEIEPEFAPLPKDVKAI
                     LRQKSLLGQIYIELTPGTRDGPKLDDGEMIPAKSVVEPVELDEIISLFNKETRDNFQG
                     WIREISIAIDNGRGEDLSNAIGNLDDFAAAGSQTLKVLDDQEPALRRLVKNTGITLNA
                     LTERRGQLRELVVNVNDFFGALASRNDRLAETIAVLPTFLDESRLTLNRLRTFTRETH
                     PLIRDLIPVAFQLRPTLRDLGALAPDLKNVFHKLDPVITESERNLPAAQRFLEGAKPL
                     FPALHTYLPELNPILSYINYSQETLANFIHAASASLNATMPPLSPDEGPRHYLRQFSI
                     SNTRGVQLSQTRPTYERGNSYPLPNYLKRMRPLGMTEAFDCNNTGFPGDGSREDSAQG
                     EPPCFVQPPQLWDGKKFPHVLRGNKIVSKPVGNEGTADPAVR"
     gene            complement(19078..20415)
                     /locus_tag="VF517_13245"
     CDS             complement(19078..20415)
                     /locus_tag="VF517_13245"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053949.1"
                     /translation="MILAIRKRLRDFIAIAALAAIALGTAYYILNEQRLRIPGLEEKP
                     FTLKAEFSDAQGVMPGQGQTIRVAGMRIGDIGSVKLENGRALVTMEIDKEHEDVVHTD
                     ATALLRPRTGLKDMFIELDPGSKSAPVMKKNGRIRIENTAPDVDSDEVLSSLDSDTRA
                     YLQLLINGAGKGLKGRGNDLREVLARFGPLHRDLARVNSAFAERRRELARLIHNYGST
                     VTELSTRDRELSTLVESQSQALERWASEDQNVSAAVARLPGTLRTVESALVKVNQLGQ
                     EIPPGLNAMRPVVRQLHQTNLQVRPFAREATPILRDQVRPFIRAARPFIRNVRPAARD
                     LANASPDVREAFREINRFFNIGAYNPNGKEPVPNDFAAAQQREEGLLFWLGWLAQNTN
                     SVFSTGDASGPFRRITATASCSTFQNLVNVEPASGALLNLNQLLADQKLCPTG"
     gene            complement(20412..22100)
                     /locus_tag="VF517_13250"
     CDS             complement(20412..22100)
                     /locus_tag="VF517_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053950.1"
                     /translation="MASTPFSNMPRGMAQKAGITPLKAGLIFLVLAILATYFAFSKAN
                     PFAQPYELYAVFENANELKERSPVRIAGVEVGKVKSIEPIADSGMAKVRMEIEEDGLP
                     IKKDAQVKVRQRIFLEGNYFVDLQPGTADSPARKSGSTIPPAQTASPVQFGDVLGALQ
                     HDTRSDLQTFLKEYAKGVEGRGSRGFNMAVKYWEAAYRNTAISTRATLGQRPGDLGRV
                     LKGQGKVFGALSRDEESLKLLVTSLNRLAAAFAREDDNLQATIPALRDVLRVGRPALA
                     SLNQAFPTLRAFAREALPAARSSSTTLDVQIPFITQARRLVSRAELRGLVADLRPTVP
                     DLARLNRSQTESLEQTRLIASCQSNVLLPFSTTPVPDPDFPNASGESFSETGPHGLVG
                     LSGESRMHDANSPIFRVLAGGGGSTLSVQSQAGEQLYAQTDLPIEGVRPIKPARTPVY
                     RPNIPCETQEPPDMNAPSGPGGDPVTPTPTLLPKYNALQKRSKADLDKVLFAWDRMNK
                     GKPYIDPLEFSDLGEQIQARKLGFKRNAKGMFVPPNELDSSETKANAGDGSGVR"
     gene            complement(22103..23629)
                     /locus_tag="VF517_13255"
     CDS             complement(22103..23629)
                     /locus_tag="VF517_13255"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053951.1"
                     /translation="MRTGRQASIVASPVLIGALTLLVVIVAVFLAYNANAGLPFVPTF
                     DVRAELEGGQNLVVSNDVRVGGYRVGSVDKIVPGVNPRTGKATAIVHMKLDKSVEPLP
                     KDSTVYVRPRSNLGLKYVELTLGKSTETMQAGDTIPVAQSRTPIELDEYFSTFDEKMR
                     QNTQTALEGYGNALAGRGQSINLAIREFVPLFTHLTPVMTALSDPNTRLNQFFRATAR
                     FSGEIAPVARQYSELFGNMATTFEALGRDENALRATIERGPPTLDAGIQSFPEQRPFL
                     ADSERLFAALQPVADEFSASLPQLREALVVGRPVVRRSPPFYNRTANVFRSLRDLVEQ
                     PATLLGLKDLTATVKIAAPLVEFVAPYQSVCNYTVFWLTGLAEHVSEDVRFGTAQRSG
                     LKSDNRTQDNRISTSDSERPADIPDNQDPQTARDPIGDPLTTLHRQNYAPAIDAQGNA
                     DCQTGQTGYLDGPLAEGFRYPPTNDPNRLGGSHVVLDPDTPGLAGTTRKGVKNLRDLP
                     "
     gene            complement(23626..25248)
                     /locus_tag="VF517_13260"
     CDS             complement(23626..25248)
                     /locus_tag="VF517_13260"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053952.1"
                     /translation="MRRLIALGTLLVACVLAVVLTGAGGDDAKGQKYLIEFDNAFGLV
                     EDGEVKVGGVSAGKITDFDLTDADPPKVAVEVELTGDDFDKFRRGIGPEAVDGVRCGV
                     RQQSLIGEYFIDCQPGTKGPQVADGGRVPVKQTFSTVPLDLINNIQRRPYRERFRLII
                     SELGVGLAGRPDELNEVIRRAHPGLRETTETLDILARQRRFIKSFVEDADEVSAAVEP
                     RKRDLSRWAKETSEVAQVQASRKDSIAEQWRKLPTFLAELQPTAAQLEQTADEQIPLL
                     RKLRTAAPDLRAFLAELGPFSEDSRRSTRALGAAAVEGRRAVDESSEEVAQLRSLAED
                     LPRFAKPLRQFLQSLDDRSRSIENDPAAGRTAPPAPDKTAYKPGQGMTGFEVIWNYVY
                     FQTLAINPFDDISHLLRIILIQNACAPYAVKPTPEQAKQCGPNIGAGGPGGRAYSPGV
                     FDPDPTDGPATEAASLSPQENIERFGLRRGAGQPEAPALPGQRDISEPQVTLPPNVQE
                     LVDSLRSGKPDAPADPTGLQQSGQLLDFLMSP"
     gene            complement(25245..26666)
                     /locus_tag="VF517_13265"
     CDS             complement(25245..26666)
                     /locus_tag="VF517_13265"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8053953.1"
                     /translation="MAEALGPERPRAPIARVAALAAVAGAVLLVAYLLFMDGGGGYRV
                     KLEFLNAQQLVYGNLVERGGVQVGSVVKREITDDGHALIEIKMNEDQPKLREGTTAII
                     RQGSLSSVANRYIELHMPPEDKAGDDIPDGGTIHSDKTTTGVEIDQFFNTFDKPTRRA
                     IQGFYRGNYANYKGRGIQANEGWKYLNPSLAGSTALFEELNKDTPVLENFLVNSSRFV
                     TALSERQEELTPLVSNLNRTTRALANEKEALAEAIERFPDFMRQANTTYVNLRATLDD
                     VDPLVEASKPVARKLRPFLSQLRPFANEARPTIRDLSQILRRKGDSNDLTELNRTYPS
                     LAQIAVETRDRNGKDRLGAFPQLTKALTDSAPVVAHGRPYTVDLIGWFDDYSTTGAYD
                     ALGSFSRVQTFVNALSAQLFGGVIPREQRGAALKALLQTEEFKRCPGGAEAPAPDGSN
                     VWSEEEQKQLDCLEEDRAVGPRP"
     gene            26832..29195
                     /locus_tag="VF517_13270"
     CDS             26832..29195
                     /locus_tag="VF517_13270"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEX8053954.1"
                     /translation="MRSVGVRALGLALIAMTCTAATAAAAAPEIKVLSNRADLISGGD
                     ALVELSPRGSGGVQGVMVDLDGRDVTAAFAERPGGRWMALLDGLKAGANVLTARAPDG
                     SASRLTITNHPIGGPVIDGPQIQPWKCAAGATDPQCNRPVRVEYRYKPSGGGSLQTYD
                     PANPPSDVATTTTDQGKTVPFIVRHEVGALDRDEYRIAVLWDPSKPFTPWAPQEGYNH
                     KLVVNHGFSCDTAYEQAAAPDVLNETALARGFAVMSHALDNAGHNCNILTQAEALIMT
                     KERVVEELGEIRYTIGSGCSGGSLVQQQVANAYPGFYQGLTPACSFTDAWSSAMQYVD
                     YVGLRNYFENPNKWGVGVAWGPDDIAAVEGHPNPVNAITFTEVIPFSGEPTRSCPGVP
                     MEQVYDENTNPKGVRCTLQDYMRNIFGPRDPSVWESVEKKLGYGFAGRPYDNVGIEYG
                     RKALEAGTISPEQFIDLNAKAGGGDIDVTLGPARSVADRPALERVYRSGAVNQGTHLD
                     QVAIIDLRGPDPGAFHDVYRTYVMRARLIREHGHAKNQILWRGQVPLLGDTNYANEAI
                     IAMDRWLAAVEKDKRGVPLPQKIVEDRPADLVERCTNGSGQELPAEACDASVQAYASP
                     RIVAGMPLTDDTIKCDLQPLRRSSYPGFSDAQFAELQKIYPTGVCDYSKPGPDRVPTV
                     PWLSYRSGPGGVPLGDPPRSTSVTPAGCLRGSSRTVAISRKLRGQRVRSVRVNANGKV
                     RLVRRKGRLYAVVDLRKLRGKTMTLRLSRRLADGKLVKTKHSRLVCK"
     gene            complement(29234..30436)
                     /gene="sufD"
                     /locus_tag="VF517_13275"
     CDS             complement(29234..30436)
                     /gene="sufD"
                     /locus_tag="VF517_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX8053955.1"
                     /translation="MASAVTARPLPPLPQFKGRPGWEFTDLSKVEVDAYEPALPGNPH
                     AVERAVALFDPPDESHQLAEVDGAVLAGAVEVDGAYVGPLDSAPAAASQLIGTVVSSD
                     DPFVVRNDAARNQGACVYVPRGVTLSAPAMLAGIQEAGGRAVNRRTLIVLEEDAQAEV
                     WEQYLSAGDELDGLFNAVVEIVVGPNARLRYVCGQGLSERSLVFGTQRAVVDANGSLE
                     WIALGFGGGTGRMSMETELRGPGAEARVTGAYATRRRQHVDFDTTQEHAAAHTTSDLA
                     FRGVLADRSSSVWRGMIKVDPGAQQTDAFQDNRNLLLSKRAHADAIPGLEILANDVRC
                     THAAAVAQIDRDQLFYLMSRGLPRDAATRLIIDGFLQSLVERFGEGALHDAVSSALER
                     RLAAILDS"
     gene            complement(30429..31874)
                     /gene="sufB"
                     /locus_tag="VF517_13280"
     CDS             complement(30429..31874)
                     /gene="sufB"
                     /locus_tag="VF517_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX8053956.1"
                     /translation="MTTPPDTAVRDTSAPELAGIGENYEERFGFHDAENYLYKAPKGL
                     NREIVEKISEFKSEPQWMRDFRLKALDHFLARPMPTWGSPMLAQVDFDNIHYFVRASE
                     RAERDWDDVPDDVKKTFDRLGIPEAERKFLAGVGAQYESEVVYHQVREDLEQQGVQFL
                     DMDSALREHEDIVREYFATIIPANDNKLAALNSAVWSGGSFVYVPPGVHVEMPLQAYF
                     RINTENMGQFERTLIIADEGSSVHYVEGCTAPTYSSDSLHSAVVELIAKPGARIRYTT
                     VQNWSQNVFNLVTKRAVAEADATVEWVDCNLGSKLTMKYPSVYLMGERAHGEILSIAF
                     AGEGQHQDAGGKIVHAAPHTTSNIFAKSISKDGGRSSYRGLLEVAKGATEARSTVVCD
                     ALLLDEDSRSDTYPTIRIGEDDVNVGHEASVSKVSEEQLFYLQSHGIPEEDASKMIVN
                     GFIEPIAKELPMEYAVELNRLIELQMEGSIG"
     gene            complement(31877..32194)
                     /locus_tag="VF517_13285"
     CDS             complement(31877..32194)
                     /locus_tag="VF517_13285"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX8053957.1"
                     /translation="MPDLIDICPLDELPPGGMRLFAHEDIEIGVFNCAGEVLAIEDRC
                     SHDDGPLVEGELDQGACTIECPRHGSRFDLRTGRPLTLPAYTPVDTFPVSVADGIIRV
                     EID"
     gene            complement(32234..33919)
                     /locus_tag="VF517_13290"
     CDS             complement(32234..33919)
                     /locus_tag="VF517_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053958.1"
                     /translation="MRLRTALVIAAVALGLMAPAAHAGDPIMPLSQVKPGMRCTAYTV
                     IRGTEITSFDAEVMDVVEQRPYAPSILVRVSGPAVADGGVAAGFSGSPIYCKDDQGTA
                     RNIGAIAEVVGDYGNDLALATPIEEILREPVDPPPGRARVRAAAARGAPVTPLTVAGL
                     SRPVERALVATARRGPRPIFAAPKAPAPSEPFVPLRPGSSVSVGFSSGDIWVGGIGTV
                     AYVDGTSAWVFGHPFEGVGRRSLLMQEAYVHTVVGNPDPSLGSYKLAAPGRDIGTITN
                     DGLSAVAGTMGILPRRTELEVTARDPQLARSETTRADVADESAVDLPSGYSPLAFVGP
                     LAVADAAARVLRAVPARESGALCARIELLGQPEPLRFCNRYVVQAGGGVGGGMADDLA
                     SAIASIDAAEYAPLAVERVEADLDVHRGARQAYMLGASMPRSVRRGSTVPVSLRARIV
                     RGGLRRFDFRLRVPRGLEPGSHVLTLEGADTDEGGELGGELGGIVIFDEDGEEAERPA
                     RSFAELRAQIDQIARWDGVTARFAGGGGGRVRAYLDPDVRIGGSIKLRLRVRR"
     gene            complement(33952..34380)
                     /locus_tag="VF517_13295"
     CDS             complement(33952..34380)
                     /locus_tag="VF517_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX8053959.1"
                     /translation="MDALYREAILDHYKHPHNFGEIDDADLEFEDTNPLCGDELKIQL
                     KVGDDGRVETVRFSGQGCAISQASASMISDEIKGMSVEQLRKLDKEAILELLGIDISA
                     TRMKCALLSLKVIKSAALGAPVDWETGEYSGGGSRGPAGL"
     gene            complement(34380..35609)
                     /locus_tag="VF517_13300"
     CDS             complement(34380..35609)
                     /locus_tag="VF517_13300"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEX8053960.1"
                     /translation="MTTEAAQRQAPALDVDRVAAEFPILRRGITYLDSAATAQKPQAV
                     LDAVMSSMAEHNANVHRGVYPLAQESTEAFEGARHTVARWINAAYEEVVFTKNVTEAI
                     NLVAHSWGTHNVGPGDTVLLTEMEHHSNIVPWQLLAQRTGAELRYVPVISGRLDMDAL
                     DRELARSPKLLAVVHVSNTLGTINDVETIVARAHRMGTRVLVDGAQAAPHFALDMQRI
                     GADFYGFTGHKVYGPSIGVLYGKRDLLESMPPFLGGGDMISRVDFQESTWNQLPWKFE
                     AGTTPFTEAVGLAAALDWLGALGIENVAAHESDVTAYALERLGEVPGLTIFGPRSLPR
                     GPAVSFALEGVHPHDVAEILGREEICVRAGHHCTQPLMRRLGVPATSRASFGVHNSRA
                     DVDKLVEGLAEVRRIFA"
     gene            complement(35606..36373)
                     /gene="sufC"
                     /locus_tag="VF517_13305"
     CDS             complement(35606..36373)
                     /gene="sufC"
                     /locus_tag="VF517_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX8053961.1"
                     /translation="MADLEIRNLHVRAGEKEILKGLDLVVRAGQVHAMMGPNGSGKST
                     LANAIMGHPNLEVTEGQILLDGQDMTEADPDERARAGLFMAFQYPVSVPGVTVTKYLR
                     TVMNAHREEKGEDPVSLKDFRRTVEDAMKLVEVPREFAARYLNEGFSGGEKKRMEILQ
                     LALQQPRIAVLDETDSGLDIDALRIVANGVNSLHGPDMGVLIITHYQRILHMVQPDSV
                     HVLYEGRIVKEGGPELVGLLEERGYGWIREEVEAAAA"
     gene            complement(36419..36883)
                     /locus_tag="VF517_13310"
     CDS             complement(36419..36883)
                     /locus_tag="VF517_13310"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8053962.1"
                     /translation="MTFSAKTEYGVRLMVALGRDAQERPTSLKAIAEAEGLPLSYLEH
                     VVANLKRAGLVTSSRGAHGGYRLARPAGSIAMDEVVLALEGAIAPMECFVQVPTERVA
                     CSHEGARAHACSTRLLWMRVQSGVVRALQGTTLGELVEFESRQEPRAEAVVA"
BASE COUNT         5131 a        13175 c        13416 g         5513 t
ORIGIN      
        1 cgggcgcggg cgccgcggcc gcgggcgtag ccgcgagctc cgcgggcgcc ggctccggcg
       61 acggcacgcg gtccgccgct gcggggggcg gagcggccac cggctccgtt gccgccggct
      121 ccgcgggctc ggccggcgcc gcggggtcgt acagcgtcac cgtctccggg agcccgttgg
      181 taccgagcga gggctccacc gccagccccg cctcggccag ccaggcctcc acgttgcggc
      241 gcgccgtcgg tgtcgcccgg cgcaggccga agccgccgag gagatcctcg gcggagatcg
      301 agacgcggcc gcccccggcg gccacgcgct cgcgcagatc ttgctcaggc cagctcgaca
      361 cgcgggtctc tctcagtcga gttcatatag cgcagtcgac caccgacggc gcacctgccg
      421 ggcgcgaggc gcgtggcggc gcgtcagggc gccgccacgc gatcctcgga gacccggtct
      481 agccgagctg cggcgtgggg cgccggcgca tccgcagcgc agcagcgcca ccgatgcagg
      541 cgacaccgcc gagcagcagg taccaggcct cgaagcctgt ctgcgccagg cggacgcgct
      601 gaccgatgag acgctcgcag cgctcaccct tcagcgtgtt gccctgcttg tccttgatgc
      661 ggccggcgtc gcagtcggcc tgcgtcactg ccgaggtgct ctgcgccatg accggagcgg
      721 ccgtgactac gagtgccact gcggtgacga gcgccaccag cgcgatcatt ccgcggaagc
      781 ggcggcgtac gagcgtcatt cggtctccct tcctctctgt cgatgaaccg aacgcggcca
      841 acctacaccc gcatggggtc ggctcgccgc ctcgacgggg cgtcttggcg cgccggccgt
      901 cagcgcaggc cgcgcagcac gcgccgcgcc acgccgcgga tcgtctcggt cccgtagacg
      961 tgcgagggat tcccgtcggt gagcacggcc agcgccacgc gccggtcgcc gctctcgatc
     1021 agcgccgact ggttgacgag gttccgccgg cccaacggcc gccagccgcc cttgaagaag
     1081 acccgccagc cggacgggat cgccggcgga acgccccagc gctgctcctc gatgatcgac
     1141 gcgagcagct tgcgcgcgta cgcccggtgg cgctccggca cgagcaggtc gatgcgggcg
     1201 aagtagcgcg cctggtcggc cgcggtgatc agcgtgtcgc cccatcgcgc cttgagcacg
     1261 aagcgcgtca tgcgcgcgcg cctggccagc gcgtagaccg cacccggccc gaggatgccg
     1321 tacacgcggg acgcccggtc gttgtcggag cggacgatca tcggtccgag cagccggcga
     1381 tcggacgtcg agagcgcgcg gccgcgaatc cggttcaggt aggcgacgag gaacatcgcc
     1441 ttgacgacgc tcgcggagcg atgctgctcg tccacgtgcc agccgcgcag ccgtccgtcg
     1501 aggtcgatca cggcgaacgc aacgcggccc gagcgcgccc gcgcgtaggc cgtcgccgcc
     1561 gccacgcgct tcgccaacgg atggcggtac gcggcggccg cgcgcgtgct cgggtcgaac
     1621 agcggcttgg cctggtcccg cgccgtcggc gcgccgccgt tcacgtccac gaacttcggc
     1681 gggtccggga gcggtgcccg cgcggccggg cggaacagcg gcggcgcgtc gggcgcgcgc
     1741 gggccctcct gcgcatgggc ggggaccgcg gcggcggcgg tcaggagagc gacggccaat
     1801 gacgcacggc gcctcacacg ccgatcgtac ggcgaaagcc ggccggccgt caggggaacg
     1861 gcgggagcgg gaacggtggc tgctcttccg gctcctcctc gggcggcgcc tccggctgcg
     1921 ggtccgcccc cggctgggcg gccggtagcg cgatcggccc gccgggcgcc gcgatctgct
     1981 tcttcgcgag cgccttcgcg gcccactgca ccagcgcccc gtaggtcgcg cgcgggctcc
     2041 cgtcgagcgt gaccaggccg aagtcgaacg ccgcgcccgg gtagcccgac ggcggagcga
     2101 cgagcccgta ctggagcatc tgccgcactc gcttgttctt ctgcgcgatc gtgaacgccc
     2161 gcggcaggta ggcggcgcgc ttcttctccg gcagcttcgc gcgcggaccg gtcgcgaagt
     2221 agccgaactc gctgaggaac acgtcgggcg ccctgcgctt cttcggcccc cacagggccc
     2281 gcgcccgcgc ggccttgtcg agcgccgtgg tcagccgcga cagcgtgccc atcgtgacgt
     2341 tgtccagccc cctgtacttg tactccggcg ggttcctgaa gtcgtacgga tggtgcgcgt
     2401 agccgtcggt ggcgaggcga ccgcgaagcc ccacgggcag cttcgccagg cagggcttgt
     2461 cgagcttgta cttcggcgtg aggcagagcg tgcgccgcag gaaggtgagc ggggccatcg
     2521 cgcgagtgcc gtggtagggc gcggtctcgc cgaacagcac ggccgcgccg gggtcggcct
     2581 gcttgatcgc cgcgtacccg gcgacgtaca gggcgcggta gagctgcggc gcctccgcac
     2641 tcgggcgcag ccagccgtcg tgattgggct cgttccagat cgagtagcgg tccacccgtc
     2701 ccctgaagcg cgtggccgcc gcgcgcgcga aggccgcgaa ctgcgccggg tcgggccgcg
     2761 tcatgctcag ctccctgtcg ccggtcgcga agcgcggcgc cggcccggcg aggtcgagct
     2821 gcacccgcat ccccgcgcgc gcggccgcgt cgatcagctc ctcgtagatg ccccagtagt
     2881 aggtgggctg ctgcggcggg tcgacggcct cgcgctgctt gcccatcacg ccggcccagt
     2941 tgacgaacac gcgcagccgc gtgaccccca gctcgcgcgc gttctggagc gcgggatcgc
     3001 ggtcgtagta gtgacgcgcg agcagcacgg ggtcgtcgga catcgcgacc tccatccgct
     3061 tgcccgcctg cgccgcgggt gcacccgcga gcgcgaccag agtcgcgcac gccaaagcga
     3121 gccccgttat ccgcattccc ccgcgcacca gcgtcagggt agccccgccc gccgctaccc
     3181 tgcgccacgt gatcgaggtg gagcacacga ccgtcgagac cggcctcccg ctcgtgcgtc
     3241 tgtcggtccc cggaacccgc gcggtcacgg cgctcgtggc gttcgacgcg ggtgcccgca
     3301 ccgagcgtcc cgaggagaac ggcatggcgc acttcctcga gcacctggtg ttcaagggcg
     3361 gcgagtcgtt cccgacctac cgcgacgtga acgagacggc cgagcggctc ggcgcgcagc
     3421 tcaacgcgta cacctcgcac gacatcgtcg ccttccacat cacctgccgc tcggaggccg
     3481 tgatggaggc gctcgacctg ctgacggact tcgtcggccg cgcgcggatc gacgccgacg
     3541 agctcgatcg cgagcgcggc gtggttatcc aggagatcgc tcgctcgaac gacgcgccgg
     3601 cgagcgtcgc cgatcggctg atcgacgcgg ccacctacgg cgagcacccg ctcgggcgca
     3661 gcgtgctcgg gcccgaggag cacctgcgca ccttcacgcg cgacgcgatc gtggccttcc
     3721 gcgagcgcca gtgggcgtcg gcgcgcggcg gcgcgttcct cgccggcgac ctcacgcacc
     3781 tcgacgccga cggcgcggcc gagctcttca cccgcttccc cgcccgcaac ggcacgcccg
     3841 aggccgaccc ggcgccgagc ttcgagcgcc gcgtgcgcgt cgagcgccgc gacacccagc
     3901 agtcgcacct gcgcctgcac tggcggccgg ccgccgatcc gaccgacctc cgccagcgtg
     3961 ccgcgctctc ggtctactcc acgctgctgg gcggatcgat gggctcacga ctgttcgacg
     4021 agatccgcga gcagcgcggc ctctgctacg cgatccgttc ctacgggacc tcgcacgccg
     4081 acgtgtcgcg gctcgacgtc tcgtccggac tcgactcgac gaagtgcccc gaggcctacg
     4141 agcgcatccg cgagatcatc gccgacctcg ccgagaacgg cccgcgcgac ggagaggtgg
     4201 aacgagcccg ctcgtacacc gccgggtcga ccgtgctggc gctcgagagc acgcgagtcg
     4261 tggcgagtcg cgccgcttcc cagaaggtca ccttcgggga gatctcctcg ccggacgaga
     4321 tgatcgcggc gctcgacgcg gtcaccgaga acgacgtgcg cgaggtcgcg gcgcaggtga
     4381 ccggcgagcc cgtggtggcc tgcgtcggcc cgcacgaggc cgccgacttc gagtagcggc
     4441 tagtccgcgg gctcgatctc gacgccgcgg cgcgtggccc gcaccacgcg gcggccggcc
     4501 agcagctcga gcagctcgtc gccgaccagg tcgcggcgcc agccgcgcag ggtgcgcacg
     4561 tcgggctcgt ccgacccggc ccttgcggcc acgacgatct cggtgaggtc ggctcgcgac
     4621 gccacgagct cgtaggccag cccggcctcc ccaacccgct ggcgcaccac cgactcgcag
     4681 agcgcgatcc gggcggcggc gccgggatcg gggtcggtcc gggtgaactc ctcggccggc
     4741 ggcggctcgc ggtcgcggcc cgtgcggatc acctcgagga cggcgttgcc gcgacggcgg
     4801 cccaggtccg ggcggacgcc gcggatcgcc gtgagcgcgt cgatcgactc cggctggcgg
     4861 cgggcgatct ccacgagcgc gacgtccgag aggaccgagc cgacgggccg gtcctcggtc
     4921 gccgcggtgc gctcgcgcca gtcggccagc gcgtaggcca cggcgcgctg cttcggccgc
     4981 agccggctca cgcgcggcag gcgccggtag gcctcgtcgg cgtcgcgctg atcgtcgatg
     5041 ccctcgagcc gacggcactc ctcgcgcgcc cagtcgagcc ggccggcctt cgcgagccgc
     5101 tcctggagcg tgtcgctcag cgccagcaga tcctcgacat cgcccttcgc gtactcgatc
     5161 tgctcgtcgg tgagcggacg gcggtcccag cgcgtgaagc ccgcgctctt gttcagctgc
     5221 actccggcga ccttctgcag gaggttcccg tacccggcct gcgcgctgaa cccggcgaag
     5281 cccgccgcga cctgggtgtc gaagacgtta gtcggcccca ctccccacgc acgtcgcagg
     5341 atcggcacgt cctgcccgcc cgcgtggatc acgacctcga cctcggggtc cgccagcacc
     5401 gccgcgagcg gctcgtggtc gaacccctcg atcgggtcga gcacctcgat gtgcggctcg
     5461 ccgttgttat cgcgcggcgc gaccgcgacc tgcacgaggc agagcagcgg cctgtagcgg
     5521 ccctcggaga cgaactccgt gtcgatcgcg atgcgcccgt gctcgcgggc gcgcgcggcc
     5581 agctcggccg gacccatcgc ctggtctctt tgcgctgctt ccaccggata gcctcggaac
     5641 gatgcgcagg gtagtggccg ccgcgcggcg cgcgggcttg ctggtgcggc tgctgccgcc
     5701 gcgagtcgcc tggttctact tccgcgcgcg gcgcacggcc gcccgcagcg gcgacgactg
     5761 gtcgctcaag tcagccacca agccggagtc gctcgcgctg cttctcaaac tggcgcgcgg
     5821 ccgccgagcc gtggtcgaga tcggcaccgg cacggcctgg acgacggccg cgctggcgct
     5881 cgccgacccg cgccgcaacg tggtgagcta cgaccccgtc atgcgccccg agcgcgaccg
     5941 ctacctggcg atcgcgggcc cgtcggccac cgcccgcatc gagctgatcg cggccggcgg
     6001 cgaggccggg ccggcgcccg agggccccgc tcccgacttc gtgttcgtgg acggctcgca
     6061 cgagcgcgag ctcacgatca gcaccttcga ggcatggcgc ggggcactgg cgccgggcgg
     6121 cgtgatcgcc ttccacgact acggaaacga gctctacccg ggggtctccg aggcgatcgc
     6181 ggagctgggc atcgaggggc gcgtggagcg cgacgtgttc gtgtggagcg ggtctctacc
     6241 ctctgaaggg tgatcgtcac catcaccccg cacgccgtcg agaaggtccg cgagttccgc
     6301 gcgaacgtcg aggatccgga cgcgcaggcc atgtgggtcc gcgtcaccgg cgtccaccgc
     6361 ggcgagttca cgtacgacct cgcgctgcgg ccgctgagcg cggccgggcc gggcgacgag
     6421 gtcgagcacg tcgacgggct ggcggtcgtc gttgcgggcg aggacgtcga ctcgctgcgc
     6481 ggtgccacga tcgactggtc cgacgacctc atgcgcggcg ggctcacagt gtcgaacccg
     6541 aaccgcccgc aggtcgtcac gcccgcgctc ggcaacggcg agctgagcgg cgacgtggag
     6601 gaccgcgtgg cgcaggtgat cgaccgccgg atcaaccccg cgatcgcatc gcacggaggc
     6661 cgcgcgacgc tcgaccacgt ggtcgacggc atcgcctacg tccgcctagg cggcggctgc
     6721 cagggctgcg gcatggccac ggtcacgctc gaccagggga tcgagtccgc gatcctcgag
     6781 tcggtccccg agatccgcca ggtggtggac gtgaccgacc acgccgccgg cgagaacccc
     6841 ttctaccagc cggcttaggg gccgtcgccc gtcagctttg aaggaaagcg cgcaaggcga
     6901 gcgtgttggc gtcctcgggg cctaacaccc gctccatctc agccgcaacg tcagtcagca
     6961 gctctaccgc gtccgcgtgg cgtccagcct tggcgaacgt gacgccgagg ttcgcacgat
     7021 acgtcaggct atgcggatgg gcggcgccga gtgtggattc cgcccccgcg ataacgctct
     7081 cccgtagggc gatggcctca tcgatccggt gtgcacgctc aagcgatgcg gcgagattgt
     7141 ttttcatgat caggacgtct ggatgcaacc gcccgaggat ctcctctgca cacgacaacg
     7201 ccgcactacg taaggcgatc gcctcacccg cgcggccttc cgcgtcgtat gcgcccccca
     7261 ggatcgatcg tgcggccaga gtatcggggt gattagggcc gaggatcgcg ctcgccacct
     7321 caagtgtccc ttccatcagc ctgacagcgt ccgcagaccg gcccgcttcg gtcagcgcgg
     7381 agcccaggct gatccgggcg gttagcgtcg tcgggtactc ggcgccgagc gtgcgctcgc
     7441 gcgtcgcgat caccgcctcg tacagagcga tcgcatcgtc ggcgcgcgac gccgccatgt
     7501 atgcacccgc caagccagca cgtgccgtca gcgtgtcttc gtgatcatca cccagcaggc
     7561 gcttcctggc agcaatgacc ctctcgaaag ccgcgatcgc cgcttcggtg cgtcccgccg
     7621 agtggtatga cgcggcgagg ttcgcgcgga ccataagcgt cgagggatga tccgagccga
     7681 aaacgcgctc atagtcctcc aagacacgtt cttcaaggtc gagcgcgtcc ttcgttcgcc
     7741 cagcggccca caccgcgttc gccaggttcg ccagcgccgc gagggtgcgc tcgtgtgcgg
     7801 gacctagcgc atgctgtgag tactcaaggg cggactccgc catcggaact ccgcgacgca
     7861 cgtcacgtac ctcaagcagg tagccacacg cgcgcctaag gacctccgcg acgtcggtgc
     7921 gctccggcct cggctcgatt gtgtgcgcca tcgcggccgc atgcggaagc agctcctcgc
     7981 acctcggcca ttccgagggc acccgtcggt ccttggggaa tgccactagc actgcgcgtg
     8041 cggcgaacgc ggggccctcc tcgtcgcccc ctagccgtgc ctggtcgcga acggtctttt
     8101 ggaggagccg atggacgctt aggctcgcct caccgacctc taccagcgac agcccgtgca
     8161 gcgcgttgaa ggcgtccgac aagcgtttcc gctcacgaat gttgttgggg tcgcccagca
     8221 acacacgaaa aaggggcagc ggaatcttgt caggagccaa gtaactggcc atgccgagga
     8281 ccgcaggcgc cagcgacgcg cgctcggccg ccgcgtcgat cgacacctgc caggttgacg
     8341 cgaccgtctg ggagtagaac gcctcgggac tctcgtcgaa gacctctgcg ctcggcaact
     8401 cggtcagcat cttcaagtac tcgtcgaacg acgtgccgga cgagcagtac gcgcccgcgt
     8461 gcgccagcgc gagcggcagc cccccgagcg cccgggtgag gcgcagcgcc gcttcgcgtt
     8521 cgccatcgcg acccgcgcgc ccgacgagga agtcggcgcc gacctcgtcc tcgaatacgt
     8581 ccacgtcaag caacgcgccg aactcaccga ccgcgcgatt gcgcgaggtc acaagcacat
     8641 tgccgttgcc ggtggccggg cagcagaccg ccaactgctc gggtgtcgcc acgttgtcga
     8701 gcacgagcag ccagcgcgcc ccggcgttct ctagcacgcg cactgccacc tgcgcgcggt
     8761 cggccggtgc cagaccttca acctcctcgc cgagctccaa ggcaagatcg gcgaggtcgg
     8821 cgatgccgcc gtcctcggcg cggatccacg cgacgacgtc gtagtcatcc acgtgcttct
     8881 gcacgtagcg cgcggcgagc tgcgacttgc ccacgccgcc gagcccggtg atcgcctgcg
     8941 taacgatcgc gcgcgaatcg tcggccagcg cgcgctcgag cgtctcgagc tccggcccgc
     9001 ggccgctgaa gtgcttcacc acatggggca gccggaagcg cacgcgccgg cctgcgggcc
     9061 gcgcgacgtc gctccacgaa tggttagcag cgtggaatgc ccggtttgcc cccatggcct
     9121 ccgcgagctg atcgcggacc gcgacccacg ggacggccag cccgccgtcc gggctggacg
     9181 ggctgcgggt ggccacgagc actccgcata cggccttcga ccggacgttg agcagcggcc
     9241 ccccgctcat tccgggcttg accgtgtccg aggcgaggtc gatgaacggc gtcgggtgct
     9301 gcgacttggt gccgcggtag tcgagcgcgg ctggcgtaag tagaaccgat cctccctcct
     9361 ccgggtagcc gtagctctgg aagctgtcgc cgtaggtggg catgtcctcg tcgagggcca
     9421 cacagggatg tcccgcagcc tccacgcgca gcagggcgat gtccgggtag tccgccgcga
     9481 ggtccgggac cggccgcccg cggtcgcaga gcttcagctc gagccgcgcc ggcacgggcg
     9541 cggcgtccgg tcgccacagg acttcgagct cctcgacatc ccccgtcacg tgcgcgcagg
     9601 tcaccaccag accgggcgcc aggaagaagc ccgcgcccgg cagcgcccct cccacgactt
     9661 ggaccgtgga ggcacgcaga agtgcctcga gactcatgcc ggagccgggt tcacccactc
     9721 gagcgtgacc gtcagcgaag ccgcgccctg ggactcgaat atgagcgcgc tgagcttgcc
     9781 cgtcttgacc gtgaggctca cgccgaacgc ggctgtcgcc ttcgttgcgc cgagcgcggc
     9841 gagcttgtcg cgcacgatcg ccgccacctc gccgatcggc tcgagcgcct cctccagctc
     9901 cagctcgttg cgcccgacgc tgccgatgcc cgacgcgccc tccgccacgc gaacctgcac
     9961 caccccgcca cccggcaacg ccgcgtcgac gaccgtatct ccgttcatgc ttgcctcctc
    10021 cgttcaggca agtttgaccg aacggcgcgg gaggcgcccg cccgtcgcca gccacgagcc
    10081 ggcgaggatg agcaccagcc cgaccacggc gccgaggccg acctcctcgt cgagcacggc
    10141 caccccgagc gcgatcgcca cggccggcgc cacgtaggtg atcaccgccg cgcgtcccgg
    10201 tcccgcctcg gcgatcagca cgctccagag cacgaacgca agcgcggtgc agaacaggcc
    10261 gagcaccacg atcgagacga gcgcgtcgcc cgtgggcgcg tcggccggcc acgtgagcgc
    10321 cgccggcagc gtgagcacca gcgcggccac ggccaggctg gcgcccatcg tcgcgcgcgc
    10381 gtcgacctcg ccgagcttgc ggttcagcac catcggcccc gcggcgtagc cgagcgcggc
    10441 cagcaggatc gccagggcgc ctagcagctc gtcgcttcgg ccggccacgt cgatgcccac
    10501 cagcgcgacc acccccgcca gccccaccac caggccgacg agccgcatac ccccgacgcg
    10561 ctcctccgcg tcgaagcgca gtgcgagcag tgccacgaac agcggcaccg ccgcgatcag
    10621 gatcgccgcg agcgacgacg ccacgtgctg ctcaccagcg gcgatcagcg ggaacggcag
    10681 cgagatctcc acgaccgcga acagcacgat ccaggtccag tgcccgcgca gctccaccag
    10741 cttgccggcg cgccaggcca gcgtgagcag gacggcggcg ccgagcacca gccgcgccca
    10801 cgacaggaac cacggcgaga ggccgtcgtc gaccgcgacc ttgatgaaca ggtacggaat
    10861 gccccacagg accgacatgg ccgcgaacac ggcccaggcg cgagagctca agcggtggtc
    10921 gggcggtcca cgcgggtgtc cgccaccggc tcgccctcgg cgcggatcac gtgcagcgct
    10981 gcgttgacct gggcgaggtg cgtgaaggcg tgcgggaagt tgccgaggtg gcgtccggtg
    11041 tccgggtcga tgtgctcggc gtagaggccc agcgggctgg ccagggcgag caggcgctcg
    11101 cacagctcgc gggcgcgttc cacctcgccg atctccacga aggccgacac gagccagaac
    11161 gagcagaccg tgaacgtctc gccctcgacc gcgaagtcct cctcctccgg ccgctggcgc
    11221 atcacgaggc cgtcgaccac cagctcgtcg gcgatcgcct cgaccgtgcg cctcacgcgc
    11281 tcgtcgtcac ccgggaggaa gcggaccagc ggcatcagga gcagcgaggc gtcgagcgcc
    11341 tcggtgtcgt agtgctgagc gaacacgccg cggtccgaga gcgcgttggc gatcacgtcc
    11401 tcgcggatcg cgtcggcgca accctgccac tcctcggcga gcccgtcctc ctggcgcagc
    11461 tcggcgagcc gggcaccgcg gtcggcggcc acccagcaca tcaccttcga cgaggtgtag
    11521 tggcgcggct cgccgcgcgt ggcccagatc ccgcggtcgg gctcccgcca gtcgctgacc
    11581 gcgcgcgtga cgagctcgtt gacgatcggc cagatccgct cgggcatctt gtcgcggctg
    11641 cgcgtgtgca ggtaggccgc gtcggccatc gcgccccaca cgtcgtgctg cgtgctgcgg
    11701 tgggccgcgt tgcccacgcg caccgggcgc gatccctcgt atcccgaaag ctggtcgacc
    11761 gtgtgctcgg agaggtcgcg ccggccgtcg atcgcgacga tgttctggag ctcctggccg
    11821 tcctcggcca gctcggcgag gaagtagaag aagtcgtcgg cctcccagtc gaagccgagg
    11881 ctgtagctgc cccacagggc gaacgcggcg tcgcgcatga agctgtagcg gtagtcgtag
    11941 ttgcgctcgc cgccggacac gcgcggcagc gaggtggtgg ccgccgagac gatggcgccc
    12001 gtcggcgcgt aggtgaggcc gcggagcgtg agcgcgctgc gctcgagctg cgcgcgccac
    12061 gggtggtcgg ggaactcgcc gcgcgcgagc cactcgcgcc agtaggtgtt ggtctggtcc
    12121 atcatgccga acgcgtcgtg gtagctcttg gggccgccgt gctcggacca ggagagtgcg
    12181 acgtatgcca cgtcgccctt gcgcatggtc gtccgcgcgg tcgcgcgcga gccctcgaac
    12241 ccgacccgca tgtcggtgtc gagcccgagc tcgaagtcgt ccttgccccc gcgcgcgacg
    12301 gcctccgtgt agccggagcc ggtgtactcc cactcggccg cgcgctcgcc gtagccgaac
    12361 accggctcgc actcgagctg gagttccacg atcccctggg cgcagcgcgc cgtgcgcacg
    12421 agcacgtggt cggcctccca gtcgctcggc gggcggtgct ggcggtgcgc acgctcctcg
    12481 acgtggtgcc acggacccac cgtgagcgcg tcgcgcacca tcagccagcc cgtgcgcgtc
    12541 ttccaggtgg tctcgagcac cacgccgccg ggcaggtagc gccgcgcggc cgggacctgg
    12601 gcgtcggtcg ggccgagccg gaagcggccc gcgtcgcgat cgaggatcgt ggcgaagacg
    12661 gccggcgagt cgaactgcgg cacgcagagc cactcgacgt tgccgctagg cgcgaccagc
    12721 gccgcaacct caccgtcgga caggaagccg tagtcgccga tcggcgggaa cgagctgcgg
    12781 gcgtggagag cctggttctg gatcgcgcga gcttagccgc gcgggcggcg cctcagcgga
    12841 gccagaagct ggtctgctta cgcgcgcccg gccagttcag gccgatgtgg atgtggtcgc
    12901 ggtgagcggt ggtgtcgtcg atcttcttgg gccggccggc gctgtcgtag caggccgagt
    12961 agggccgcat cccgtcgccg cccgaccacc acgagcggca gttccagatc agctcctgga
    13021 tgcccatgcg gcgggcgagc gcgtggcggt tgccgaccct gtcggtggcg aggaagaggc
    13081 ggatcagccg cgaggcctcg cgcttgtccg cggagcggtg cacgttcagg tgccagtcga
    13141 tcgcgcggcc ctcggcgtgc aggctcgcgc tgtccttgcc ccagcgctcg cagcgcatcg
    13201 tcccccagaa ggcgccgccc gcgttcgcct cgagccaggc cttcagcgcg agggtgccgg
    13261 gcggaggcga cttgcggcag ctcttggcgt ggtcgtagcg gtaggggtcg atcgcggtcg
    13321 cggcgagggc gtcggcgggg ttcgtcgtgg ccgcgggcgc tgtcgcggga gcgagcgcga
    13381 ggagcgcgag cgcgagcagg gcgatgcggc gggccatgct tgagctatcg gcacggggcg
    13441 gcgcgggctt gccacccgaa cgggttacag ctcgagcagc gctgccatca gcgcgagggc
    13501 cacctcggcg cgctggtgga tcgactcggc gagcacgaac tcgtccgggt tgtgcgcctg
    13561 gccgccgcgc gggccgaagc cgtcgagcgt gagcgggacg gccgacgaga cgtggctcgc
    13621 gtcgctcgcg ccgccgcgct cgaccgggat caccggccgc ccgagcacct cggccgtggc
    13681 cgccagcagg tcgcgcgtgg tctcgcgcga gtccatcccc ggccagagcc tcacgatctc
    13741 cgcctcgagc cgggcccctc cgacctcgtc gggaacctcc gcaagcaccc ggtggaacgc
    13801 cgcggcgtcg tcggcgcgca tgtcgcagaa gagctcgccg ccggacggga ccacgttgaa
    13861 cgcgtcgccg ctcgtgagga tcgtggggac cgcgctgagc cggtccgggc cctgcgggtc
    13921 gtgatgcgcg gccaccgcct gagcggccgc ggccagcgcc agcagggcgt tgcgcccgcg
    13981 gtcgggtgcc gagccggagt gcgacgagag cccgtgcgcg acgaccttca gcgtgctggc
    14041 ggccttgcgc cgcacgatca ggccctcctc cccctccggc ccgtgctggc cggcctcgaa
    14101 gcacaggcag gcgtcgaagc cctcgaagcg gtgcacgtgg tgcaggctcc cgacccgcca
    14161 ctcctcgtcg ttgacgagca gcagcgcgat ctccgcatag gcctccgggc gctcggcgag
    14221 cacctcgaac agcgccagcg agagggccac gccgcccttc atgtccacgg tgcccgatcc
    14281 gatgatccgg tcgccgcgac gctcgatcgg gcggtgcgac tcgtgcgcga ccacggtgtc
    14341 gaggtgcccg agcaggagca cccgccgctt gcccgtgccg cgcacgtgcg ccagcaggtc
    14401 gtcggcgtac ccggccgtgg agcacgggat ccgctcgacc tcggcctccg gtggcagcag
    14461 cgccgcgcag acggccaccg cctcctccgc gccgtgcacg tcccccgagg gcgacgagac
    14521 cgccaccagc gcctccatct cgcgctcggc gcgcagcgcc atccgcgaag cgtggctctc
    14581 gacccagggg tgcatcgtcc cgacggtagc ggcggtctcg gtcagccgag cctggcgcgc
    14641 gactcgcgcg cggcggcgtt gaagacgccg cgtgccatcg tcgagcttgc gtcctgcgcg
    14701 cggcactcgt tcacctcggc gcgggcgcgc tgcgcggcct cgagctccgg ctcggccagc
    14761 gctgccagct cggccaggtg gccggcaagc cgcagctcgc cggcctcggc cagctccagc
    14821 gcacgctcgg ccagccgctc cccgccgccg gtaagccccg ccagctcgcc cgccagcgcc
    14881 gcgtcgggcg cgggcttcag gttggccggg ttgccgtcat accagccgcc gaactggcgc
    14941 cagaggttgc gcacgatgaa ctcgggctcg tcgtagatcg ggcgcaggta cggcttctcg
    15001 aggaggttcg cgggcgggcg caccgcgtgc aggatctggt cgagcggcgc gccctcgttc
    15061 atcagcgcga gggtctgctc cacgagcgac tcgagcagcg ccgcgctgtc ctcgagcgcc
    15121 gcgcgcacgc gctcggcgcc caccaccggg aggccgtggc cggggagcag cagctcggcg
    15181 tccagctcgg ccatcgcccg cagcgcctgg acccactcgc gcgggtagcg ctggaccttc
    15241 tgcgggttgc ccgcgttcgg cacggcccag atgaacaggt cgccgcagca gatcgtccgc
    15301 cggccgggga tccaggtcca cgtgtagtcg tcggtctcgc ccttcgcgtg gtgcagctcg
    15361 aagcgctcgc caccgatctc gagctcgagc tcgtcccggt aggtcttgtc cgggtagcgg
    15421 tagtcgagcg gccactcgag ctcgtccaca ccgaactggc ggcggttgat ggcggcgttg
    15481 tagccggcgg tgagccggta gcggtcgaag cgggccggca tgttctcgtg cgcgaccacg
    15541 atcggcggga tccagccgcg gtcgacggcc tcctcctccc agagcggcag cccgaacacg
    15601 tggtccacgt gcccgtgcgt gaagaccacc gtgtgcacac aggtgtccgg ccgccagccg
    15661 cggatcgact cgaacgccca ctgcgcgagc gcgaagctgc cgctgtcgaa cagcacgagg
    15721 ccctcgtccg tctcgacggc cgtcacgttc gcgaacgacg gcaggaaggc gatgccctcg
    15781 cgcacctccg tcagctcgcc gaggaagccg accgggtggt gctcggcgag gtcggcctcg
    15841 ccgctccaca gccggtccgc gagctcgagc atgtcgggca tggcgccgcg agcctaccgc
    15901 tgattgaatc ccggccggca tgtcgcgcca ggctccgatg ctccaggcgc tcgacgacgc
    15961 gccgatcacg aagcgctacg tgatcctcgc cgtcgtcacg atgttcggcg ccgtcctcga
    16021 cctgttcgac ttcttcctga tcgcgttcat cgtcccggtg atcgacgacg agtggcagct
    16081 cacgttcggg caggcgaccg cggtgctgct gagcgcaggc gtcggcgcga tcgcgggcgc
    16141 gctgatctgg ggctggctgg gcgaccgcta cggccgccgc aagccgctga tcgccggcat
    16201 cctcaccttc tcggtcgcca cgggcctgct cgcgctggca ccggacgacg gctggtggtt
    16261 cctcgcgctc gggcggatcg tggtcggcgc gggcgtggcc ggggtggcgg tgatcgcggt
    16321 cccgctcacg ctcgagttca cgcccacccg catgcgcacg accatcgtcg gcttcgtcac
    16381 caccgccacg gtcccgatcg gcatcctcgc cgcggcgctg gcggccgcgc tgctcgccga
    16441 cccgctcggc tggcgcccgc tgttcgccgt cggcgtcgtg ccggccgtgc tggcgctgtt
    16501 catcaagtgg tacgtgccgg agtcgccgcg ctggctgatc gaccaggggc gaccggagga
    16561 ggcgcggcgg tcgatcgcct acctgctgat ggagccggag gagtcgctgt cgctcgaggt
    16621 gccgccgcgc gaccgcgagc ccgagatcgg cttccgccag ctcttccgct accgcaacag
    16681 cctgctggtg acgtgcaccg cgtggttcgg tgcgtcggtg gcggtgtcgg ggctgatcct
    16741 ctgggggccg acgttcctga aggagatcct cgagatcgac tccgacgagg ccgcgctcct
    16801 gttcgtgttc gtcacgctcg gctcgttcgc cgggcggctg ttcttctcgt tcgctcccgc
    16861 gcgcttcggc cgccgcgcct gcggagtggt gaccggcctc ggcgccgcgc cgctgctgtt
    16921 cctggccgcc gccagcggcg agaccgaggt cctcgggatc tcgctgttcc tggtggcgct
    16981 ggtgctggcg agcttcttcg tcgacggcgg cttcgccaac ctcgcgccgc tcacgccgga
    17041 gctcttcccg acgagcctgc gcacccaagg gctcggcctc tcctgggcgt tcagcgggct
    17101 cggccggatc gtcggcccgc tcgtggtggg cgcctgggcc gcggagggca acgacccgat
    17161 cgagccggag gcgacgctcg acgcgctcct ccccgcgttc agcttcctgg ccgtggccgc
    17221 gctggcggcg gggctcgtgt tcctcgtgct gaggacggag ccgcacgggc gcgatctgga
    17281 gtcgctgagc gacgagctgc gggagagcgt ggccgctccg ggcgccgcgg cggcggagcc
    17341 ggcggcgccc gcctagggct ccggccgctc cccacggcgg gcacttaggg gagggggaac
    17401 gggcgcccgc gcatcgattg gagggttatt ccgctatgcg gtacacaagg ctccaatcga
    17461 tggggagcct tcgaccctcc cccctcccca gccgccgttg gcggcaggcc ggggagccgc
    17521 agcggccgtg ggcgccagcc gcagagcgcc agccgcacgc ggtgacgagc cgcgcccgca
    17581 cgcggcggcg cgccgcgccc taccgaaccg ccgggtccgc cgtcccctcg ttgccgaccg
    17641 gcttgctcac gatcttgttg ccgcgcagga cgtgcgggaa cttcttgccg tcccagagct
    17701 gcggcggctg gacgaagcag ggcggctcgc cctgggcgga gtcctcgcgg ctgccgtcgc
    17761 cggggaaacc ggtgttgttg cagtcgaacg cctcggtcat gccgagcggg cgcatccgct
    17821 tgaggtagtt gggcagcggg tacgagttgc cgcgctcgta ggtcggccgc gtctgcgaga
    17881 gctggacgcc gcgcgtgttc gagatcgaga actggcgcag gtagtggcgc gggccctcgt
    17941 ccggcgagag cggcggcatc gtggcgttca gcgacgcgga ggcggcgtgg atgaagttgg
    18001 cgagtgtctc ctgcgagtag ttgatgtagg agaggatcgg gttgagctcc ggcaggtagg
    18061 tgtgcagcgc cgggaacagc ggcttcgcgc cctcgaggaa gcgctgcgcg gcgggcaggt
    18121 tgcgctcgga ctccgtgatc accgggtcga gcttgtggaa cacgttcttg aggtcgggcg
    18181 cgagggcgcc gaggtcgcgc agcgtcggcc ggagctggaa ggcgaccgga atcaggtcgc
    18241 ggatgagcgg atgcgtctcc ctggtgaacg tgcgcagcct gttcagcgtc aggcgcgact
    18301 cgtccaggaa cgtgggaagc acggcgatcg tctcggcgag gcggtcgttg cgcgacgcca
    18361 gcgcgccgaa gaagtcgttc acgttcacga ccagctcacg cagctgcccg cggcgttcgg
    18421 tcagcgcgtt cagcgtgatg ccggtgttct tcacgagcct gcgcagcgcc ggctcctggt
    18481 cgtccagcac cttcagcgtc tgcgagccgg cggccgcgaa gtcgtcgagg ttgccgatcg
    18541 cgttgctcag gtcctcgccg cgcccgttgt cgatcgcgat cgagatctcg cggatccagc
    18601 cctggaagtt gtcgcgcgtc tccttgttga agagggagat gatctcgtcc agctcgaccg
    18661 gctccacgac ggacttcgcc gggatcatct cgccgtcgtc gagcttcggc ccgtcgcggg
    18721 taccgggcgt cagctcgatg tagatctgac ccaacagcga cttctgccgg aggatcgcct
    18781 tcacgtcctt cggcagcggt gcgaactcgg gctcgatctc catctcgacc acgttgccgc
    18841 cctcgcgcgc gaggctgagg ttcttcacct tgccgatgtc gaggccggcc atgcggatgt
    18901 cggcctcctt cacgagcagc gccgactcgg gcatcttcac cttcacgcgg tagccctccg
    18961 ggcgcagcgg caccgggccg ccgaacgaga accacagcca gagcagcagc ccgaagcagg
    19021 acagcgccca catcaccatc acgaagatct tggtcgggct cggagcttgc ttgttcatca
    19081 gccggtcggg cagagcttct ggtcggccag aagctggttg aggttcagga gcgcgccgga
    19141 ggcgggctcg acgttgacga ggttctggaa cgtcgagcac gaggcggtgg cggtgatgcg
    19201 ccggaacggc ccgctcgcgt cgccggtgct gaagacgctg ttggtgttct gcgcgagcca
    19261 gccgagccag aacaggaggc cctcctcgcg ctgctgggcg gcggcgaagt cgttcggcac
    19321 cggctccttg ccgttcgggt tgtaggcgcc gatgttgaag aaacggttga tctcgcggaa
    19381 cgcctcgcgc acgtccggac tcgcgttggc caggtcgcgc gcggccgggc gcacgttgcg
    19441 gatgaacggg cgtgccgcgc ggatgaacgg gcgcacctgg tcgcgcagga tcggcgtggc
    19501 ctcgcgggcg aacgggcgca cctggaggtt ggtctggtgg agctggcgga cgaccggccg
    19561 catcgcgttc agccccggag ggatctcctg gccgagctgg ttgaccttca cgagcgccga
    19621 ctccaccgtg cgcagcgttc ccggcaggcg cgcgaccgcc gccgagacgt tctggtcctc
    19681 ggacgcccag cgctcgagcg cctggctctg cgactcgacc agggtgctga gctcgcggtc
    19741 gcgcgtggac agctcggtga ccgtcgagcc gtagttgtgg atcaggcggg ccagctcgcg
    19801 gcggcgctcc gcgaacgcgc tgttcacgcg cgcgaggtcg cggtgcagcg gcccgaaccg
    19861 cgcgagcacc tcgcgcaggt cgttgccgcg ccccttgagg cccttgcccg ctccgttgat
    19921 caggagctgg aggtaggccc gcgtgtcgct gtcgagcgac gagaggacct cgtcggaatc
    19981 cacgtccggc gccgtgttct cgatccggat ccgcccgttc ttcttcatca ccggcgccga
    20041 cttgctgccc gggtcgagct cgatgaacat gtccttcagg ccggtgcgcg ggcgcagcag
    20101 cgcggtcgcg tcggtgtgga cgacgtcctc gtgctccttg tcgatctcca tcgtgaccag
    20161 ggcgcgcccg ttctcgagct tcacgctgcc gatgtcgccg atccgcatgc cggccacgcg
    20221 gatcgtctgg ccctggcccg gcatgacgcc ctgcgcgtcg gagaactcgg ccttgagcgt
    20281 gaacggcttc tcctcgaggc ccgggatgcg cagtcgctgc tcgttgagga tgtagtacgc
    20341 ggtgccgagg gcgatcgcgg ccagcgccgc gatcgcgatg aagtcgcgca gccgcttgcg
    20401 gatcgcgagg atcatcggac gccgctcccg tcgccggcgt tggccttcgt ctcggagctg
    20461 tcgagctcat tcggcgggac gaacatgccc ttggcgttgc gcttgaagcc gagcttcctc
    20521 gcctggatct gctcgccgag gtccgagaac tcgagcgggt cgatgtacgg cttgcccttg
    20581 ttcatccggt cccaggcgaa gaggaccttg tcgaggtcgg ccttcgagcg cttctggagc
    20641 gcgttgtact tgggcagcag cgtgggcgtg ggcgtgacgg ggtcgccgcc cgggccgctc
    20701 ggggcgttca tgtccggcgg ctcctgcgtc tcgcacggga tgttcggccg gtagaccggc
    20761 gtccgtgcgg gcttgatcgg ccgcacgccc tcgatcggca ggtcggtctg ggcgtagagc
    20821 tgctcgccgg cctggctctg caccgacagg gtgctgccgc cgccaccggc caggacgcgg
    20881 aagatcggcg agttcgcgtc gtgcatgcgg ctctccccgg agaggccgac cagcccgtgc
    20941 ggcccggtct cggagaagga ctcgcccgag gcgttgggga agtccgggtc cgggaccggc
    21001 gtggtcgaga acggcagcag cacgttgctc tggcacgacg cgatcaggcg ggtctgctcg
    21061 agcgactcgg tctggctgcg gttcaggcgc gcgagatccg ggaccgtcgg ccgcaggtcg
    21121 gccacgagcc cgcgcagctc cgcgcgcgag acgaggcggc gcgcctgcgt gatgaacggg
    21181 atctggacgt cgagcgtggt cgacgacgag cgcgccgcgg gcagtgcctc gcgcgcgaac
    21241 gcgcgcagcg tggggaaggc ctggttcagc gacgccagcg ccgggcggcc gacgcgcagc
    21301 acgtcgcgca gcgccgggat ggtcgcctgg aggttgtcgt cctcgcgggc gaaggccgcg
    21361 gccagccggt tcagcgaggt gacgagcagc ttgagcgatt cctcgtcgcg cgacagcgct
    21421 ccgaacacct tgccctggcc cttgagcacg cggccgaggt cgccggggcg ctggccgagc
    21481 gtggcgcgcg tggagatcgc ggtgttgcgg taggccgcct cccagtactt gacggccatg
    21541 ttgaagccgc gcgagcccct gccctcgact cccttcgcgt actccttgag gaaggtctgg
    21601 aggtcggagc gggtgtcgtg ctggagcgcg cccagcacgt cgccgaactg gaccggcgag
    21661 gcggtctgcg ccggcgggat ggtcgagccg gacttgcgcg ccggcgagtc cgccgtgccg
    21721 ggctggaggt cgacgaagta gttgccctcc aggaagatgc gctggcgcac cttgacctgg
    21781 gcgtccttct tgatggggag gccgtcctcc tcgatctcca tccggacctt cgccatgccg
    21841 gagtccgcga tcggctcgat cgacttcacc ttgccgacct cgacgccggc gatccggacc
    21901 ggcgagcgct ccttcagctc gttggcgttc tcgaagaccg cgtagagctc gtagggctgc
    21961 gcgaacgggt tggccttgct gaacgcgaag taggtcgcga ggatggccag cacgaggaag
    22021 atcaggcccg ccttgagcgg cgtgatgccc gccttctgcg ccattccgcg cggcatgttg
    22081 gagaagggag tcgaggccat cgctagggga ggtccctcag gttcttgacg cccttgcggg
    22141 tggtgccggc gaggcccggc gtgtccgggt cgagcacgac gtggctgccg ccgagccggt
    22201 tcgggtcgtt cgtcggcgga tagcggaagc cctccgccag cgggccgtcg aggtagccgg
    22261 tctggccggt ctggcagtcg gcgttgccct gcgcgtcgat cgccggcgcg tagttctggc
    22321 ggtgcagcgt ggtgagcgga tcgccgatcg gatcgcgcgc cgtctgcggg tcctggttgt
    22381 cggggatgtc cgccgggcgc tcggagtcgg aggtgctgat gcggttgtcc tgggtacggt
    22441 tgtcggactt caggccggag cgctgcgccg tgccgaagcg cacgtcctcg gagacgtgct
    22501 cggcgaggcc ggtgagccag aagaccgtgt agttgcagac cgactggtac ggcgccacga
    22561 actcgacgag cggcgcggcg atcttgaccg tcgcggtcag gtccttcagg cccagcagcg
    22621 tcgcgggctg ctcgacgagg tcgcgcagcg acctgaacac gttcgccgtg cggttgtaga
    22681 acggcggcga gcggcgcacc accggccggc cgacgaccag cgcctcgcgg agctgcggca
    22741 gcgacgccga gaactcgtcg gcgaccggct ggagcgcggc gaacaggcgc tcggagtcgg
    22801 ccaggaacgg ccgctgctcg gggaagctct ggatgccggc gtcgagcgtc ggcgggccgc
    22861 gctcgatcgt ggcgcgcagc gcgttctcgt cgcgcccgag cgcctcgaag gtcgtggcca
    22921 tgttcccgaa cagctccgag tactggcgcg cgaccggagc gatctcgccg gagaagcgcg
    22981 cggtggcccg gaagaactgg ttgagccgcg tgttcgggtc cgacagcgcc gtcatcaccg
    23041 gcgtcaggtg ggtgaagagc ggcacgaact cgcggatggc gaggttgatc gactggccgc
    23101 ggccggcgag cgcgttgccg tagccctcga gcgccgtctg ggtgttctgg cgcatcttct
    23161 cgtcgaacgt cgagaagtac tcgtcgagct cgatcggcgt gcgcgactgc gcgaccggaa
    23221 tcgtgtcgcc cgcctgcatc gtctcggtgc tcttgccgag ggtcagctcg acgtacttca
    23281 ggcccaggtt cgagcgtggc cgcacgtaga cggtcgagtc cttcggcagc ggctcgaccg
    23341 acttgtcgag cttcatgtgg acgatcgccg tggccttccc ggtccgcggg ttcacgcccg
    23401 gcacgatctt gtccacggac ccgacgcggt agccgcccac gcgcacgtcg ttcgagacga
    23461 ccaggttctg gccgccctcg agctcggccc gcacgtcgaa ggtcggcacg aacggcaggc
    23521 ccgcgttcgc gttgtaggcg aggaacacgg cgacgatgac cacgagcaac gtcagggccc
    23581 cgatcaggac cggcgaggcg acgatggatg cctggcggcc ggtcctcatg gcgacatcag
    23641 gaagtcgagg agctgtccgg actgctggag cccggtcggg tcggcgggag cgtccggctt
    23701 cccgctgcgg agggagtcga cgagctcctg cacgttcggc gggagggtga cctgcggctc
    23761 ggagatgtcg cgctggccgg gcagcgccgg tgcctcgggc tggccggcgc cgcgccgcag
    23821 gccgaagcgc tcgatgttct cctgcgggct gaggctcgcg gcctcggtgg cggggccgtc
    23881 ggtcgggtcg gggtcgaaga cgcccgggct gtaggcccgg ccgcccggtc cgccggcgcc
    23941 gatgttcggc ccgcactgct tggcctgctc gggcgtcggc ttgaccgcgt acggggcgca
    24001 cgcgttctgg atcaggatga tccgcagcag gtggctgatg tcgtcgaacg ggttaatcgc
    24061 gagcgtctgg aagtacacgt agttccagat cacctcgaag ccggtcatgc cctggcccgg
    24121 cttgtacgcg gtcttgtcgg gggccggcgg cgccgtgcgg ccggcggccg ggtcgttctc
    24181 gatcgagcgg ctgcggtcgt ccaggctctg gaggaactgg cggagcggct tggcgaagcg
    24241 cggcaggtcc tcggccagcg agcggagctg cgcgacctcc tcgctgctct cgtcgaccgc
    24301 gcggcgaccc tcgacggccg cggcgccgag cgcccgcgtc gagcggcgcg agtcctcgga
    24361 gaacggcccg agctcggcga ggaacgcccg caggtcgggc gccgccgtgc gcagcttgcg
    24421 caggagcggg atctgctcgt cggcggtctg ctcgagctgc gccgcggtgg gctggagctc
    24481 ggctaggaac gtcgggagct tgcgccactg ctcggcgatc gagtccttgc gcgacgcctg
    24541 cacctgggcc acctcggagg tctccttggc ccagcgcgag aggtcgcgct tgcgcggctc
    24601 cacggcggcg ctgacctcgt cggcgtcctc cacgaagctc ttgatgaagc gccgctggcg
    24661 ggcgaggatg tcgagagtct cggtggtctc gcgcaggccc gggtgcgcgc ggcggatgac
    24721 ctcgttcagc tcgtccgggc ggcccgcgag cccgacgccc agctcgctga tgatgagcct
    24781 gaagcgctcg cggtaggggc ggcgctggat gttgttgatc aggtcgagcg gcaccgtcga
    24841 gaacgtctgc ttgaccggca cccggccgcc gtcggcgacc tgcgggccct tggtgccggg
    24901 ctggcagtcg atgaagtact cgccgatcag cgactgctgg cggacgccgc agcgcacgcc
    24961 gtcgaccgcc tccggcccga tcccgcggcg gaacttgtcg aagtcgtcgc ccgtcagctc
    25021 gacctcgacg gcgaccttcg ggggatcggc gtcggtcagg tcgaagtcgg tgatcttgcc
    25081 ggcgctcacg ccgccgacct tcacctcgcc gtcctcgacc agaccgaagg cgttgtcgaa
    25141 ctcgatgagg tacttctgac ccttcgcgtc gtcgccgcct gcgccggtca gcaccaccgc
    25201 gagcacgcag gcgaccagca gcgtgccgag ggcgatcagg cgcctcatgg ccgcggcccc
    25261 acggcccggt cttcctcgag gcagtcgagc tgcttctgct cctcctcgga ccagacgttc
    25321 gagccgtccg gcgcaggcgc ctcggcgccg cccgggcagc gcttgaactc ctcggtctgg
    25381 agcagcgcct tgagcgccgc gccgcgctgc tcacgcggga tgacgccgcc gaagagctgc
    25441 gccgagagcg cgttcacgaa cgtctggaca cgcgagaagc tgccaagcgc gtcgtaggcg
    25501 cccgtggtcg agtagtcgtc gaaccagccg atcaggtcga ccgtgtacgg gcggccgtgc
    25561 gcgacgaccg gcgcgctgtc ggtgagcgcc ttcgtgagct gcgggaaggc gccgagccga
    25621 tccttgccgt tgcgatcgcg cgtctcgacc gcgatctgcg ccagcgacgg gtaggtgcgg
    25681 ttcagctcgg tgaggtcgtt ggagtcgccc ttgcggcgga ggatctggct gaggtcgcgg
    25741 atagtcgggc gcgcctcgtt cgcgaacggc cgcagctgcg agaggaacgg ccgcagcttg
    25801 cgggcgaccg gcttggacgc ctcgacgagc gggtccacgt cgtcgagcgt cgcgcgcagg
    25861 ttcacgtagg tcgtgttcgc ctggcgcatg aagtcgggga agcgctcgat cgcctcggcg
    25921 agggcctcct tctcgttggc cagcgcgcgc gtggtgcggt tgaggttgct gacgagcggg
    25981 gtcagctcct cctggcgctc ggacagcgcg gtgacgaagc gcgagctgtt gacgaggaag
    26041 ttctcgagga cgggcgtgtc cttgttcagc tcctcgaaca gcgcggtcga gccggcgagc
    26101 gacgggttga ggtacttcca gccctcgttc gcctggatcc cgcggccctt gtagttcgcg
    26161 tagttgccgc ggtagaagcc ctggatcgcc ctgcgcgtcg gcttgtcgaa cgtgttgaag
    26221 aactggtcga tctcgacgcc ggtggtcgtc ttgtcggagt ggatcgtgcc gccgtccggg
    26281 atgtcgtcgc cggccttgtc ctccggcggc atgtgcagct cgatgtagcg gttggcgacc
    26341 gaggagagcg agccctgccg gatgatcgcg gtggtgccct cgcgcagctt gggctggtcc
    26401 tcgttcatct tgatctcgat cagcgcgtga ccgtcgtcgg tgatctcgcg cttgacgacc
    26461 gagccgacct gcacgccgcc gcgctcgacg aggttgccgt agacgagctg ctgcgcgttc
    26521 aggaactcga gcttcacccg gtagccgccg ccaccgtcca tgaacagcag atacgcgacg
    26581 agcagcacgg cgccggcgac cgcggcgagt gccgcgacgc gggcgatcgg agccctcggg
    26641 cgctcaggcc ccagtgcctc agccaatcga ggtctcgatg atcgtctgca agttacgtat
    26701 ctccccgggc ggcccgagcg gccgccgccg ggtgaaagct actcgctgga cccgcccgat
    26761 gcgctggatt gaggagcccc cggcgcgcct taacgtaatg ttcatgccgg atgaggagga
    26821 gcttcggagg gatgcgatcc gtgggggtcc gcgcgctggg gttggcgctg atcgccatga
    26881 cctgcacggc ggcgacggcg gccgcggccg ctcccgagat caaggtcctc tccaaccgcg
    26941 cggacctgat ttcgggcggg gacgcacttg tagagctgtc tccgcgcgga tcgggcggcg
    27001 tgcagggcgt catggtggac ctcgacggcc gcgacgtgac ggccgccttc gccgagcgcc
    27061 cgggcggtcg ctggatggcg ctgctcgacg gcctgaaggc cggcgcgaac gtcctcacgg
    27121 cgcgtgcacc ggacggatcg gcctcccggc tgacgatcac aaaccacccg atcggggggc
    27181 cagtgatcga cggtccgcag atccaaccgt ggaagtgcgc ggcgggcgcg accgacccgc
    27241 agtgcaaccg gccggtgcgg gtcgagtacc gctacaagcc gagcggcgga ggctcgcttc
    27301 agacctacga ccccgcgaac ccaccgtcgg acgtcgccac caccacgacc gaccagggca
    27361 agaccgtccc gttcatcgtc cgtcacgagg tcggcgcgct cgaccgcgac gagtaccgga
    27421 tcgccgtgct ctgggacccg agcaagccgt tcacgccgtg ggcgccgcag gagggctaca
    27481 accacaagct cgtcgtgaac cacggcttca gctgcgacac cgcttacgag caggccgcgg
    27541 cgcccgacgt gctcaacgag accgcgctcg cgcgcggctt cgccgtgatg tcgcacgcgc
    27601 tcgacaacgc gggccacaac tgcaacatcc tcacccaggc cgaggcgctg atcatgacca
    27661 aggagcgggt ggtcgaggag ctcggcgaga tccgctacac gatcggcagc ggctgctccg
    27721 gcggctcgct cgtgcagcag caggtggcga acgcctaccc cggcttctac cagggcctga
    27781 cgccggcgtg cagcttcacg gacgcgtggt cctcggccat gcagtacgtg gactacgtcg
    27841 ggctgcgcaa ctacttcgag aacccgaaca agtggggcgt gggcgtggcc tgggggccgg
    27901 acgacatcgc ggcggtcgag gggcacccga acccggtcaa cgcgatcacg ttcaccgagg
    27961 tcatcccgtt cagcggcgag ccgacgcgtt cgtgtccggg cgtgccgatg gagcaggtct
    28021 acgacgagaa caccaatccc aagggcgtgc gctgcacgct ccaggactac atgcgcaaca
    28081 tcttcggccc gcgcgacccg agtgtctggg agtccgtcga gaagaagctc ggctacggct
    28141 tcgcgggccg gccgtacgac aacgtgggca tcgagtacgg gcgcaaggcg ctcgaggcgg
    28201 ggacgatctc gcccgagcag ttcatcgacc tgaacgccaa ggccggcggc ggcgacatcg
    28261 acgtgaccct cggccccgcg cgctcggtgg ccgaccgccc cgccctcgaa cgcgtctacc
    28321 gcagcggcgc cgtgaaccag gggacacacc tcgaccaggt ggcgatcatc gacctgcgcg
    28381 gccccgaccc cggcgccttc cacgacgtct accgcaccta cgtgatgcgc gcgcggctga
    28441 tccgcgagca cggccacgcg aagaaccaga tcctctggcg cggccaggtg ccgctgctcg
    28501 gcgacacgaa ctacgccaac gaggcgatca tcgccatgga ccgctggctg gcggccgtcg
    28561 agaaggacaa gcgcggcgtg ccgctgccgc agaagatcgt cgaggacagg cccgcggacc
    28621 tggtcgagcg ctgcacgaac ggctcggggc aggagctgcc cgcggaggcg tgcgacgcca
    28681 gcgtgcaggc ctacgccagc ccgcggatcg tggccggcat gccgctcacc gacgacacga
    28741 tcaagtgcga cctccagccg ctccggcggt cgagctaccc cggcttctcg gacgcgcagt
    28801 tcgcggagct gcagaagatc tacccgaccg gcgtgtgcga ctactcgaag ccggggcccg
    28861 accgggtgcc gacggtgccg tggctgtcct acaggtcggg gccgggcggc gtcccgctcg
    28921 gcgatccccc gcgctcgacg tccgtcacgc ccgccgggtg cctgcgcggc tcgtcgcgca
    28981 cggtcgcgat ctcacgcaag ctgcgcgggc agcgcgttcg gtcggtgcgc gtgaacgcga
    29041 acgggaaggt ccggctcgtg cggcgaaagg gccgcctcta cgcggtggtg gacctgcgca
    29101 agctgcgcgg caagacgatg acgctgcgcc tctcccgccg gctcgccgac ggcaagctgg
    29161 tcaagacgaa gcactcgcgg ctcgtctgca agtagttctg aacagttgac caccccatag
    29221 gggcgtcaag tgttcaggaa tcgaggatcg cggccagccg gcgctcgagt gcgctcgaca
    29281 cggcgtcgtg cagggcaccc tcgccgaagc gctcgaccag cgactggagg aagccgtcga
    29341 tgatcaggcg ggtggccgcg tcgcgcggga ggccgcgcga catcaggtag aagagctggt
    29401 cgcggtcgat ctgcgcgacc gcggcggcgt gcgtgcagcg cacgtcgttg gcgaggatct
    29461 cgaggcccgg gatcgcgtcg gcgtgcgcgc gcttcgagag cagcaggttg cggttgtcct
    29521 ggaacgcgtc ggtctgctgg gcgcccgggt cgaccttgat catgccgcgc cagacgctgc
    29581 tcgagcggtc cgcgagcacc ccgcggaagg cgagatcgga cgtggtgtgc gcggccgcgt
    29641 gctcctgagt cgtgtcgaag tcgacgtgct ggcgccggcg ggtggcgtag gcgccggtca
    29701 cgcgcgcctc ggcgcccggt ccgcgcagct cggtctccat gctcatgcgc ccggtgccgc
    29761 cgccgaagcc cagcgcgatc cactcgagcg atccgttcgc gtccaccacg gcacgctggg
    29821 tgccgaacac gagcgagcgc tcggacagcc cctggccgca cacgtagcgc agccgggcgt
    29881 tcggcccgac cacgatctcg acgaccgcgt tgaacaggcc gtccagctcg tcgccagcgg
    29941 agaggtactg ctcccagacc tccgcctgcg catcctcctc gagcacgatc agcgtgcggc
    30001 ggttgaccgc gcggccgccg gcctcctgga tcccggcgag catcgccggc gccgacagcg
    30061 tgaccccgcg ggggacgtac acgcaggcgc cctggttgcg cgcggcgtcg ttgcggacga
    30121 cgaacgggtc gtcggaggag acgaccgtcc cgatcagctg cgaggcggcc gcgggtgccg
    30181 agtcgagggg cccgacgtac gcgccgtcga cctccactgc cccggccagc accgccccgt
    30241 ccacctcggc gagctggtgt gactcgtcag gggggtcgaa gagcgcgacg gcgcgctcca
    30301 ccgcgtgagg gttccccggg agggccggct cgtacgcgtc gacctcgacc ttcgagaggt
    30361 cggtgaactc ccagccgggc ctgcccttga actgcggtag tgggggcagg ggccgcgccg
    30421 tgacggcgct agccaatgct gccttccatc tggagctcga tcagccggtt gagctcgacg
    30481 gcgtactcca tcggaagctc cttcgcgatc ggctcgatga agccgttgac gatcatcttc
    30541 gacgcgtcct cctccgggat cccgtgggac tggaggtaga acagctgctc ctcggagacc
    30601 ttcgagaccg aggcctcgtg tccgacgttc acgtcgtcct cgccgatcct gatggtgggg
    30661 taggtgtccg agcgcgagtc ctcgtcgaga agcagcgcgt cgcacaccac cgtcgagcgc
    30721 gcctcggtgg cacccttcgc gacctcgagc aggccgcggt agctggagcg cccgccgtcc
    30781 ttcgagatcg acttggcgaa gatgttcgac gtcgtgtgcg gcgccgcgtg gacgatcttg
    30841 ccgcccgcgt cctggtgctg gccctcgccc gcgaacgcga tcgagaggat ctcaccgtgg
    30901 gcgcgctcgc ccatcaggta gacgctcggg tacttcatcg tgagcttcga cccgaggttg
    30961 cagtcgaccc actcgaccgt ggcgtcggcc tccgcgaccg cccgcttcgt aacgaggttg
    31021 aagacgttct gcgaccagtt ctggaccgtc gtgtagcgga tccgcgcgcc cggcttggcg
    31081 atcagctcga ccaccgcgga gtgcagggag tccgacgagt acgtgggggc ggtgcagccc
    31141 tcgacgtagt gcaccgacga gccctcgtcg gcgatgatca gcgtgcgctc gaactggccc
    31201 atgttctcgg tgttgatccg gaagtaggcc tggagcggca tctcgacgtg cacgcccggc
    31261 ggcacgtaga cgaacgagcc gcccgaccac acggccgagt tgagcgcggc gagcttgttg
    31321 tcgttcgccg ggatgatcgt ggcgaagtac tcgcggacga tgtcctcgtg ctcgcgcagc
    31381 gcggagtcca tgtcgaggaa ctggacgccc tgctgctcga ggtcctcgcg cacctggtgg
    31441 tagacgacct cggactcgta ctgcgcgccg acgccggcga ggaacttgcg ctcggcctcg
    31501 gggatgccga gccggtcgaa ggtcttcttg acgtcgtcgg ggacgtcgtc ccaatcgcgc
    31561 tcggcgcgct cggaggcgcg gacgaagtag tggatgttgt cgaagtcgac ctgggcgagc
    31621 atcgggctgc cccaggtcgg catcggacgc gcgaggaagt ggtcgagcgc cttcaggcgg
    31681 aagtcgcgca tccactgcgg ctcggacttg aactcggaga tcttctcgac gatctcgcgg
    31741 ttgagcccct tcggggcctt gtagaggtag ttctcggcgt cgtggaagcc gaagcgctcc
    31801 tcgtagttct ccccgattcc ggcgagctcg ggagcggagg tgtcacggac tgctgtatcg
    31861 ggcggcgtgg tcatggtcag tcgatctcca ctcggatgat gccgtcggcg acggacaccg
    31921 ggaaggtgtc caccggcgtg taggcgggga gggtcagcgg gcggccggtg cgcaggtcga
    31981 agcgcgaccc gtgccgcggg cactcgatgg tgcaggcgcc ctggtcgagc tcgccctcga
    32041 cgagcgggcc gtcgtcgtgc gagcagcgat cctcgatcgc gagcacctca ccggcgcagt
    32101 tgaagacgcc gatctcgatg tcctcgtggg cgaacaggcg catgccgccg ggcggcagct
    32161 cgtcgagggg gcagatgtcg atcaggtctg gcatgtcagc ttgttaatcc gactcggcgg
    32221 atcgggattg attctagcgc cggacgcgga gccggagctt gatcgagccg cctatccgga
    32281 cgtcggggtc gaggtaggcg cgcacgcgcc ctcctccccc gcccgcgaag cgcgccgtga
    32341 ccccgtccca gcgcgcgatc tggtcgatct gcgcccgcag ctccgcgaac gagcgggcgg
    32401 gccgctcggc ctcctcgccg tcctcgtcga agatcacgat cccgccgagc tcgccgccga
    32461 gctcgccgcc ctcgtcggtg tcggcgccct cgagcgtgag cacgtgcgag ccgggctcca
    32521 ggccgcgcgg tacccggagc cggaagtcga agcggcgcag cccgccgcgc acgatgcgcg
    32581 cgcgcagcga gaccgggacg gtcgagccgc gccggacgct ccgcggcatc gaggctccga
    32641 gcatgtaggc ctgccgcgcg ccgcggtgga cgtcgaggtc ggcctccacg cgctcgacgg
    32701 cgagcggggc gtactcggcc gcgtcgatcg aggcgatcgc gctcgccagg tcgtcggcca
    32761 tgccgccgcc cacgccaccg ccagcctgga ccacgtagcg gttgcagaag cgcagcggct
    32821 cgggctggcc gagcagctcg atccgggcgc acagcgcacc gctctcgcgc gccggcaccg
    32881 cgcgcagcac gcgcgccgcg gcgtcggcga ccgcgagcgg ccccacgaac gcgagcggcg
    32941 agtagcccga cggcaggtcg acggccgact cgtcggccac gtcggcgcgg gtggtctcgg
    33001 agcgggcgag ctgcgggtcg cgcgcggtga cctcgagctc ggtcctgcgc ggcaggatgc
    33061 ccatcgtgcc ggccaccgcg gagaggccgt cgttggtgat cgtgccgatg tcgcggccgg
    33121 gagccgccag cttgtagctg ccgagcgacg ggtccgggtt tccgacgacc gtgtgcacgt
    33181 acgcctcctg catcagcagc gagcggcggc cgacaccctc gaatggatgg ccgaagaccc
    33241 aggccgacgt gccgtcgacg tacgcgaccg tgccgatccc gccgacccag atgtcgccgc
    33301 tcgagaaccc gaccgacacc gacgagcccg ggcgcagcgg cacgaacggc tcgctgggag
    33361 ccggggcctt cggcgcggcg aagatcggcc gcggaccgcg ccgcgcggta gccacgagcg
    33421 cgcgctcgac cggacggctg aggccggcga ccgtgagcgg ggtgaccgga gcgccgcgcg
    33481 ccgcggcggc gcggacccgc gcgcggccgg gcggtgggtc caccggctcg cgcaggatct
    33541 cctcgatggg ggtggcgagc gccaggtcgt tgccgtagtc gcccacgacc tccgcgatag
    33601 cgccgatgtt gcgcgccgtc ccctgatcgt ccttgcagta gatcggcgac cccgagaagc
    33661 ccgccgccac cccgccgtcg gccaccgccg gccccgagac gcggaccagg atgctgggcg
    33721 cgtagggccg ctgctccacc acatccatca cctcggcgtc gaagctcgtg atctcggtcc
    33781 cgcggatcac cgtgtaggcg gtgcagcgca tgcccggctt cacctgggag agcggcatga
    33841 tcgggtcgcc ggcgtgcgcg gccggcgcca tcaggccgag ggcgaccgcg gctatgacga
    33901 gggcggtccg caggcgcact ggcgcaactt agcggtgaag agggcccggc gctacagccc
    33961 ggcgggcccg cggctgccgc cgccggagta ctcgccggtc tcccagtcga ccggcgcgcc
    34021 tagcgcggcg ctcttgatga ccttcagcga gagcagcgcg cacttcatcc gggtggccga
    34081 gatgtcgatc ccgagcagct cgaggatcgc ctccttgtcg agcttgcgca gctgctccac
    34141 ggacatgccc ttgatctcgt ccgagatcat cgaggccgac gcctggctga tcgcgcagcc
    34201 ctggcccgag aagcgcacag tctccacgcg cccgtcgtcg ccgaccttga gctggatctt
    34261 cagctcgtcg ccgcagagcg ggttggtgtc ctcgaactcg aggtcggcgt cgtcgatctc
    34321 cccgaagttg tgcgggtgct tgtagtgatc gaggatggcc tctcgataca gggcgtccat
    34381 tacgcgaaga tcctccggac ctcggcgagg ccctcgacca gcttgtccac gtcggcccgc
    34441 gagttgtgga ccccgaagct cgcgcgcgag gtggccggca cgccgaggcg gcgcatcagc
    34501 ggctgcgtgc agtggtggcc ggcgcggacg cagatctcct cgcggccgag gatctcggcc
    34561 acgtcgtgcg ggtggacgcc ctccagcgcg aacgagacgg cggggccgcg cgggagcgag
    34621 cgggggccga agatcgtgag tccggggacc tcgccgaggc gctcgagcgc gtatgcggtc
    34681 acgtccgact cgtgggcggc gacgttctcg atgccgagcg caccgagcca gtcgagcgcc
    34741 gcggccagcc cgacggcctc ggtgaacggc gtggtgcccg cctcgaactt ccaggggagc
    34801 tggttccagg tcgactcctg gaagtcgacg cggctgatca tgtcgccgcc gccgaggaac
    34861 ggcggcatcg actcgagcag gtcgcgcttg ccgtagagca cgccgatcga aggcccgtag
    34921 accttgtggc cggtgaaccc gtagaagtcc gcgccgatcc gctgcatgtc gagcgcgaag
    34981 tgcggcgccg cctgggcgcc gtcgacgagc acgcgggtgc ccatccggtg cgcgcgggcg
    35041 acgatcgtct cgacgtcgtt gatcgtcccg agcgtgttcg agacgtggac gacggcgagc
    35101 agcttcggcg agcgagccag ctcgcggtcg agcgcgtcca tgtcgagccg gccggagatc
    35161 acgggcacgt agcgcagctc ggcgccggtg cgctgggcca ggagctgcca cgggacgatg
    35221 ttcgagtgat gctccatctc ggtgaggagc accgtgtcgc ccgggccgac gttgtgtgtg
    35281 ccccacgagt gcgcgacgag gttgatcgcc tcggtcacgt tcttggtgaa gacgacctcc
    35341 tcgtaggccg cgttgatcca gcgggcgacg gtgtggcgcg cgccctcgaa ggcctcggtc
    35401 gactcctgcg cgagcgggta gacgccgcgg tggacgttcg cgttgtgctc ggccatcgag
    35461 gacatcaccg cgtcgaggac ggcctgcggc ttctgagccg tggccgcgct gtcgaggtag
    35521 gtgatgccgc gccgcaggat cgggaactcg gccgcgaccc gatcgacgtc gagcgcgggc
    35581 gcctggcgct gtgccgcctc ggtggtcacg ccgccgcggc ctccacctct tcgcggatcc
    35641 agccgtagcc ccgctcctcg agcaggccga cgagctcagg gccgccctcc ttgacgatcc
    35701 gcccctcgta gaggacgtgc acggagtccg gctgcaccat gtgcaggatg cgctggtagt
    35761 gggtgatgat caggacgccc atgtccgggc cgtgcagcga gttgaccccg ttggcgacga
    35821 tccgaagcgc gtcgatgtcg agcccggagt cggtctcgtc gagcaccgcg atgcgcggct
    35881 gctggagcgc gagctggaga atctccatgc gcttcttctc accgcccgag aagccctcgt
    35941 tcaggtagcg cgcggcgaac tcgcgcggca cctcgacgag cttcatcgcg tcctccaccg
    36001 tgcggcggaa gtccttcagg ctcacgggat cctcgccctt ctcctcgcgg tgggcgttca
    36061 tcaccgtgcg caggtacttc gtgacggtca cgcccggcac cgagaccggg tactggaaag
    36121 ccatgaacag gcccgcgcgg gctcgctcgt ccgggtcggc ctcggtcatg tcctggccgt
    36181 cgagcaggat ctggccctcg gtcacctcga ggttcgggtg ccccatgatc gcgttggcga
    36241 gggtggactt gccggagccg ttcggcccca tcatcgcgtg gacctgaccc gcgcgcacga
    36301 ccaggtcgag acccttgagg atctccttct cgcccgctcg tacgtggagg ttcctgatct
    36361 cgaggtccgc catttgagtc tccgtcctct gtgtagtcgt cttggtgggg ccgccgcgct
    36421 acgcgacgac ggcctcggca cgcggctctt gccgggactc gaactcgacg agctcgccca
    36481 gcgtcgttcc ctgtagcgcg cgcacgactc cgctctgcac ccgcatccag aggaggcgcg
    36541 tggagcaggc gtgcgcgcga gcgccctcgt gcgagcaggc gacacgctcg gtcggcacct
    36601 gcacgaagca ctccatcggg gcgatcgccc cctcgagggc cagcacgacc tcgtccatcg
    36661 cgatcgaacc ggccggccgc gcgaggcggt agccgccgtg cgctccgcgg ctcgaggtga
    36721 cgagcccggc ccgcttgagg ttggccacca cgtgctccag atacgagagc gggaggccct
    36781 cggcctccgc gatcgccttg agcgacgtgg ggcgctcctg cgcatcgcgc ccgagggcga
    36841 ccatgaggcg gacgccgtac tcggttttcg cggagaaggt cacgcctaaa tcctagcaac
    36901 ggggtcggat taaccggggg gtgcgccaca ccccccgtca ggggggcgca acgcctgttc
    36961 ccacctggaa tggaggccgc cgagtggccg aggcagtggc ccctccggcg gatagcggtt
    37021 gtgcgcctct cctaaggaat gcccgcaact ttgggctttt ctccgtagcc ctcggcgctc
    37081 aactatgcca ctgtcacacg gcgggagggg aatcttgagc aacgcggaga cagcactgag
    37141 cgaagaccag gacttcgtcg aggaggcctc taaaccggtc gtcccggccc cggcacggtt
    37201 cacatcgtcg gtcaagccga gcgacgagca tcggc
//
LOCUS       DASVPL010000246         6875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_383688, whole genome shotgun sequence.
ACCESSION   DASVPL010000246 DASVPL010000000
VERSION     DASVPL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383688"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VF517_13315"
     CDS             <1..513
                     /locus_tag="VF517_13315"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8053963.1"
                     /translation="QAVLNLGNAGARVKMLVRGDRLAKTMSAYLVDRIADHPLIDVRL
                     HTEVVEVNSVDGELDCVTVTGGERLPAAALFICIGGQPRTGWAEPIGVRTDRAGYILT
                     GPDLLDAGRRPEGWPLDRDPLALETSVPGLFAAGDVRHGSTKRVAGAVGEGAMAVALA
                     HRRLEELAGR"
     gene            complement(587..1564)
                     /locus_tag="VF517_13320"
     CDS             complement(587..1564)
                     /locus_tag="VF517_13320"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8053964.1"
                     /translation="MTVTDVTAVDERAAARRDPGPPVVREMTQAQRIGNVIGVVVPFL
                     GVLAAIVLLWNQAVDAVDLAILAVTYIFVGFGVTVGYHRLLTHGAFQTRPSIRYTFAI
                     LGSMSVQGSVLDWVADHRKHHAHADEEGDPHSPHVGHGSGLSGLWHAHVGWLFNNQGQ
                     ADWKKYARELYEDPGMRRIHKLFLPLTALSLLIPAMLGFALHGWTLEGFLRGLVWGGL
                     VRAFMLHHITWSINSICHFFGRRRFATDDHSTNVAWLAVPSLGEAWHHNHHAFPRAAE
                     HGLERGQIDPSGLLIRGLEKLGLAWNVVRISPERQAQKLAAGARPPARA"
     gene            1685..2386
                     /locus_tag="VF517_13325"
     CDS             1685..2386
                     /locus_tag="VF517_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8053965.1"
                     /translation="MRLRLLLASLAAAVALGGCQYPRDVEGTLDRVEGGTMRVGVSDN
                     EPWVNLDGSEPTGVEPEIVRRFAKTIDAKIQWVQGDSEQLIDALEGGQLDLVIAGITR
                     TSLLKLKAALTRPYVDTELLLAHPRGEDMPDESHDVKVAVEADSQAASLLDHKTDYGL
                     LELDSLEGHRGPALVWDYWLDDLDLESTGEDLIDEEHAIAVRFGENAMLTELERFLLD
                     REGEWRGLVNREGKP"
     gene            2383..3441
                     /locus_tag="VF517_13330"
     CDS             2383..3441
                     /locus_tag="VF517_13330"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8053966.1"
                     /translation="MRYPGGPELPDEQQQAQRKAGRLEWFTLAYMFTALVAVYFALGD
                     SQAMKAAWLEDILGLVPPAAFLIAARYRYRAPTEQMPWGYHRAISVAYLAGAIAIVAL
                     GAFILFDSSHKLIKGEHASIGMVELFDWQLWQGWLMLAVLIYTGAPPVILGLMKKPLA
                     QKLHDKVLFADAEMQRADWMTAAAAMVGVIGIGFGLWWADSVAAIVIALDVVHDGARY
                     TGRAVLNLMDSRPRTYDEKKPHPLKLQVEHELGQMDWVREAVVRMRELGHVLAVNAFV
                     VPSSDDGLPDRVEETIERLRALDWKLHDVSVTVVRTIDHVPEGESRVSRGIASFDEVR
                     RDIGLSERDVEEQGASGR"
     gene            complement(3445..4776)
                     /locus_tag="VF517_13335"
     CDS             complement(3445..4776)
                     /locus_tag="VF517_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053967.1"
                     /translation="MRRALPLLLTFVALAGLAAEATAQTRPRWDTRVLALVPRPGFPA
                     HAYVHPNGRIYAGTYTNPSGDAAPSRVFEYDGGNGTLLRSWTVEGQNLAEEHGVQAAT
                     SDARGRIVLLDKSPPRILLLDQRTSAQIPYASFGSSSIPNYAAWGPDGSLYVTDYELP
                     ILWRVPPGGGTPEKWLEDPRLDGGGEFGTTGIELAGDRTTLLVAQQSEAGGAAGNPTT
                     GRLFRVPIGPDGKAGAMTQLWESAPADGPDGFGIAKSGNVYIANLASNQIAVVGPGGN
                     EIERFPQSPGTGENGSSVPFDSPSNVSFLGTRAIVAQQSYFAGDPTKQAILDVETGEE
                     GLPELIPANAGLADAVPPSVKPATLDARRLTLTLSEPAEVDFLVERRDPGGWVVLRRS
                     FRDLAAGAHTLSLRDIAEKKRLRRALHRVTIFATDRAGNVSRDVRRYARVR"
     gene            complement(4773..6290)
                     /locus_tag="VF517_13340"
     CDS             complement(4773..6290)
                     /locus_tag="VF517_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEX8053968.1"
                     /translation="MSPKRGMNRRQLLGTTAAAAATGAAAGAAPASAASKNVRKIPQR
                     SADVIVVGAGLAGLTAARAVQKAGKSVLVIEANDRVGGRVWNHELEGGKVSERGGTFV
                     GPTQDHIIDLAKEYGIGTFSSVIEGENVYVDPDGARSTYSDTGPTGTAPLDPAILPDL
                     ATSVALLDQMSTEVPVDAPWNAPRAREFERYTLEEWIRQNSTTERFRALVPAATRPIF
                     GAEPRELSLLFVLFYIAASGDETHVGTFERNFNTREGAQERRLKGRSSQGLAQLMAAK
                     LGSGNVLLKAPVRRIVQGRSIVRVESPAVVARGKRVIVAIPPTSAGRIDYDPVMPADR
                     DQLTQRVWQGALTKVAAAYDKPWWREKGLNGTALGTSNDILVNATFDDSPEDGSPGVI
                     FGFVGGDQSRRYAGMSGSARRAKVLDEFALYFGEEARNPVEFFDTLWPDERWIRGAPV
                     GNYSPGHLLAYGPALRRVQGKIHWAGTETSTYWNGYMDGAVRSGERAAKEVLDRL"
     gene            6363..>6875
                     /locus_tag="VF517_13345"
     CDS             6363..>6875
                     /locus_tag="VF517_13345"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8053969.1"
                     /translation="MSNLAQEAIEARRAAAEGNGAASPGATGTVREATYDLLRAHGIT
                     TMFGNPGSTELPFLADFPSDLTYVLALQEAVAVGMADGWAQASGRTAFVNLHTTPGVG
                     HAMGSIVNARDNRAPMVVTAGQQSRPMMALSALLTNQDATTLPKPAVKWSYEPARAED
                     VPMAVARGLQL"
BASE COUNT          951 a         2386 c         2550 g          988 t
ORIGIN      
        1 caggcggtgc tgaacctcgg caacgcgggc gcgcgggtga agatgctcgt gcgcggcgac
       61 cggctggcga agacgatgtc ggcctacctg gtcgatcgga tcgccgacca tccgctgatc
      121 gacgtgcgac tgcacaccga ggtggtcgag gtcaacagcg tcgacgggga gctcgactgc
      181 gtgacggtca ccggcggcga gcggctgccg gccgcagccc tcttcatctg catcggcggt
      241 cagccgcgca ccgggtgggc cgagccgatc ggcgtgcgca ccgaccgcgc cggctacatc
      301 ctcaccggcc cggacctgct cgacgccggg cggcggcccg agggctggcc gctcgaccgt
      361 gacccgctgg cgctcgagac gagcgtgccc ggcctgttcg ccgccggtga cgtgcggcat
      421 gggtctacca agcgcgtggc cggcgccgtg ggcgagggcg cgatggcggt ggcactggcg
      481 catcgccggc tggaggagct ggccggccgc tagtagacgc gccccgcggg gggcgcgagt
      541 ccatttcagg gtcctatagc acccacaaat ggactctcgc cccgccctac gcgcgcgcgg
      601 gcgggcgcgc tccggcggcc agcttctgcg cctggcgctc gggcgagatc cgcaccacgt
      661 tccacgccag cccgagcttc tcgagcccgc ggatcaggag cccggacggg tcgatctgcc
      721 cgcgctccag cccgtgctcg gccgcccgcg ggaaggcgtg gtggttgtgg tgccacgcct
      781 cgccgagcga cggcacggcg agccacgcga cgttggtcga gtggtcgtcg gtggcgaacc
      841 gtcggcggcc gaagaagtgg cagatcgagt tgatcgacca ggtgatgtgg tgcagcatga
      901 acgcgcgcac caggccgccc cagaccagcc cgcgcaggaa gccctcgagc gtccagccgt
      961 ggagcgcgaa gccgagcatg gccgggatca gcaggctgag cgcggtgagc ggcaggaaca
     1021 gcttgtggat ccggcgcatc cccgggtcct cgtagagctc gcgcgcgtac ttcttccagt
     1081 ccgcctggcc ctggttgttg aagagccacc cgacgtgggc gtgccagagc ccgctcagcc
     1141 cggagccgtg cccgacgtgc gggctgtgcg ggtcgccctc ctcgtcggcg tgcgcgtggt
     1201 gcttgcggtg gtcggctacc cagtcgagca cggagccctg caccgacatg gacccgagga
     1261 tcgcgaaggt gtagcggatc gaggggcggg tctggaacgc gccgtgcgtg aggaggcggt
     1321 ggtagccgac cgtcacgccg aagccgacga agatgtaggt gacggcgagg atcgccaggt
     1381 cgaccgcgtc gaccgcctgg ttccagagca ggacgatcgc ggccagcacg ccgaggaacg
     1441 ggacgaccac gccgatcacg ttgccgatgc gctgcgcctg cgtcatctcc cggaccaccg
     1501 gaggcccggg gtcgcggcgc gccgccgccc tctcgtcaac cgccgtcacg tccgtaaccg
     1561 tcacgcgtcc ctcctcgatc gccaagccca ccccgctctt ggctcttatt ccccaaaccg
     1621 cgtacctcat gtcatgaacc cgcaaattcg gtgtccggcc gccccggcgg gtacaacgcg
     1681 tgacgtgcgc ctacgcctgc tgctcgcttc cctggccgcc gccgtcgcgc tcggcggctg
     1741 ccagtacccg cgcgacgtgg agggcacgct cgaccgcgtc gagggcggca cgatgcgcgt
     1801 gggcgtgagc gacaacgagc cgtgggtgaa cctcgacggc tccgagccga ccggggtcga
     1861 gccggagatc gtgcgccgct tcgcgaagac gatcgacgcg aagatccagt gggtgcaggg
     1921 cgacagcgag cagctgatcg acgcgctcga gggcggccag ctcgacctcg tcatcgccgg
     1981 gatcacgcgc acgtcgctcc tgaagctcaa ggccgcgctc acccgcccgt acgtcgacac
     2041 cgagctgctg ctcgcgcacc cgcgcgggga ggacatgccc gatgagagcc acgacgtgaa
     2101 ggtggccgtc gaggccgaca gccaggccgc ctcgctgctc gaccacaaga ccgattacgg
     2161 cctgctcgag ctcgactcgc tcgagggtca ccgcggcccg gcgctcgtgt gggactactg
     2221 gctcgacgac ctcgacctcg agtcgaccgg cgaggacctg atcgacgagg agcacgccat
     2281 cgcggtgcgc ttcggcgaga acgcgatgct caccgagctc gagcgcttcc tgctcgaccg
     2341 cgagggcgag tggcgcgggc tcgtgaaccg ggagggcaag ccgtgaggta cccgggcggg
     2401 ccggagctgc cggacgagca gcagcaagcg cagcgcaagg cgggccggct cgagtggttc
     2461 acgctcgcct acatgttcac cgcgctggta gcggtgtact tcgcgctcgg cgactcgcag
     2521 gcgatgaagg cggcctggct cgaggacata ctcggcctgg tgccgccggc ggcgttcctg
     2581 atcgccgcgc gctaccgcta ccgcgcgccg accgagcaga tgccctgggg ctaccaccgc
     2641 gcgatctcgg tggcctacct cgccggcgcg atcgcgatcg tcgcgctcgg cgcgttcatc
     2701 ctgttcgact cgagccacaa gctcatcaag ggcgagcacg cctcgatcgg gatggtcgag
     2761 ctgttcgact ggcagctctg gcagggctgg ctgatgctcg cggtgctgat ctacacgggc
     2821 gcgccgccgg tgatcctcgg gctgatgaag aagccgctcg cccaaaagct ccacgacaag
     2881 gtgctcttcg ccgacgcgga gatgcagcgc gccgactgga tgacggcggc cgcggcgatg
     2941 gtcggcgtga tcgggatcgg cttcgggctc tggtgggcgg actcggtggc cgcgatcgtg
     3001 atcgcgctcg acgtggtcca cgacggtgct cgttacaccg gccgcgccgt gctcaacctg
     3061 atggattcgc ggccgcgcac gtacgacgag aagaagccgc atccgctgaa gctccaggtg
     3121 gagcacgagc tcggccagat ggactgggtg cgtgaggcgg tggtgcggat gcgcgagctc
     3181 gggcacgtgc tcgccgtgaa cgcgttcgtc gtgccgtcga gcgacgacgg gctgcccgac
     3241 cgcgtcgagg agacgatcga gcgcctgcgc gcgctcgact ggaagctgca cgacgtgtcg
     3301 gtcaccgtcg tgcgcacgat cgaccacgtg cccgagggcg agagccgcgt gtcgcgcggg
     3361 atcgcctcgt tcgacgaggt gcgccgggac atcggtctga gcgagcggga cgtggaggag
     3421 cagggcgcga gcgggcgcta gccgctagcg cacccgcgcg tagcggcgca cgtcgcgcga
     3481 gacgttgccg gcgcgatcgg tggcgaagat ggtcacgcgg tggagcgcgc gcctgagccg
     3541 cttcttctcg gcgatgtccc tgagcgacag cgtgtgcgcc cccgcggcga ggtcgcggaa
     3601 gctgcggcgc aggacgaccc acccgccggg gtcgcggcgc tcgaccagga agtcgacctc
     3661 cgccggctcg ctgagcgtga gcgtgagccg ccgcgcgtcg agcgtcgccg gcttcacgct
     3721 cggcgggacc gcgtcggcca gcccggcgtt cgccgggatc agctcgggca acccctcctc
     3781 ccccgtctcg acgtcgagga tcgcctgctt ggtcgggtcg cccgcgaagt aggactgctg
     3841 ggcgacgatc gcgcgcgtgc cgaggaagct cacgttcgac ggcgagtcga acggcacgct
     3901 cgaaccgttc tcgccggtgc ccgggctctg cgggaagcgc tcgatctcgt tcccgccggg
     3961 tccgaccacg gcgatctggt tcgacgcgag gttcgcgatg tagacgttgc cggacttcgc
     4021 gattccgaag ccgtcggggc cgtccgccgg cgcgctctcc cagagctggg tcatggcgcc
     4081 ggccttgccg tcggggccga tcggcacgcg gaacagccgc ccggtggtgg ggttgccggc
     4141 ggcgccgccc gcctcgctct gctgcgccac gaggagggtc gtgcggtcgc ccgccagctc
     4201 gatcccggtg gtgccgaact cgccgccgcc gtcgaggcgc gggtcctcga gccacttctc
     4261 cggcgtgccc ccgcccggcg ggacgcgcca caggatcggc agctcgtagt cggtcacgta
     4321 aaggctgccg tccggccccc aggcggcgta gttggggatc gacgagctgc cgaaggaggc
     4381 gtacgggatc tgcgcgctgg tccgctggtc gagcagcagg atgcgcggcg ggctcttgtc
     4441 gagcaggacg atccggccgc gcgcgtcgct cgtggccgcc tgcacgccgt gctcctcggc
     4501 gaggttctgt ccttcgacgg tccacgagcg cagcagcgtg ccgttgccgc cgtcgtactc
     4561 gaacacgcgc gagggggcgg cgtcgccgct cgggttcgtg taggtgccgg cgtagatgcg
     4621 cccgttcggg tgcacgtacg cgtgggcggg gaagccgggg cgcgggacga gcgcgagcac
     4681 gcgcgtgtcc cagcgcgggc gggtctgcgc ggtcgcctcc gcggcgaggc cggcgagcgc
     4741 gacgaacgtg agcaggagcg gaagcgcccg cctcacagcc ggtccagtac ctccttggcg
     4801 gcacgctctc cggagcgcac ggcgccgtcc atgtagccgt tccagtaggt cgaggtctcg
     4861 gtcccggccc agtggatctt gccctggacg cggcggagcg ccgggccgta cgcgagcagg
     4921 tgacccgggc tgtagttgcc gaccggtgcg ccgcggatcc agcgctcgtc gggccacagc
     4981 gtgtcgaaga actcgaccgg gttgcgcgcc tcctccccga agtacagggc gaactcgtcg
     5041 agcaccttcg cgcggcgcgc ggacccggac atgccggcgt agcggcgcga ctggtcgccg
     5101 ccgacgaagc cgaagatcac gccgggcgat ccgtcctccg gggagtcgtc gaaggtggcg
     5161 ttgacgagga tgtcgttgct cgtgccgagc gccgtgccgt tgaggccctt ctcgcgccac
     5221 cacggcttgt cgtacgcggc ggcgaccttc gtgagtgctc cctgccagac gcgctgggtg
     5281 agctggtcgc ggtcggccgg catgaccggg tcgtagtcga tccgcccggc gctcgtgggc
     5341 gggatcgcga cgatcacgcg cttcccgcgg gccaccacgg ccggcgactc cacgcgcacg
     5401 atgctcctgc cctgcacgat gcgccgcacg ggcgccttca ggagcacgtt cccgctcccg
     5461 agcttcgcgg ccatcagctg ggcgagcccc tgcgacgagc ggcccttgag gcgccgctcc
     5521 tgggccccct cgcgggtgtt gaagttgcgc tcgaacgtgc cgacgtgggt ctcgtcgccc
     5581 gacgcggcga tgtagaagag cacgaatagc agcgacagct cgcgcggctc ggctccgaag
     5641 atcggccggg tggccgccgg cacgagtgcc cggaagcgct cggtggtgct gttctggcgg
     5701 atccactcct cgagcgtgta gcgctcgaac tcgcgggcgc gcggcgcgtt ccagggagcg
     5761 tccaccggca cctcggtcga catctggtcg agcagggcca ccgacgtcgc gaggtcgggc
     5821 aggatcgccg ggtcgagcgg tgcggtgccg gtggggcccg tgtcgctgta ggtgctgcgc
     5881 gcgccgtcgg ggtcgacgta cacgttctcg ccctcgatca cggacgagaa cgtgccgatc
     5941 ccgtactcct tggcgaggtc gatgatgtgg tcctgggtgg ggccgacgaa cgtgccgccg
     6001 cgctccgaga ccttgccgcc ctcgagctcg tggttccaca cgcgtccgcc gacgcggtcg
     6061 ttcgcctcga tcacgaggac cgacttgccg gccttctgga ccgcgcgtgc cgcggtgagg
     6121 ccggcgagcc cggcgccgac cacgatcacg tccgcgctgc gctgcgggat cttcctgacg
     6181 ttcttgctgg cggcgctcgc gggagcggcg ccggcggcgg ctccggtggc cgcggctgct
     6241 gccgtcgtgc caaggagctg acggcggttc atacctcgct tcgggctcat aggcgggacg
     6301 ttacagagca tggcgtgaga ggcttcccag ttcgtggcga acgctgaccg acgggagacc
     6361 tgatgagcaa cctggcccag gaggcgatcg aggcacgccg ggcggctgcc gagggcaacg
     6421 gagcggcctc ccctggcgcg acgggcaccg tgcgcgaggc gacctacgac ctgctgcgcg
     6481 cgcacggcat cacgacgatg ttcggcaacc cggggtcgac cgagctgccg ttcctcgcgg
     6541 acttccccag cgacttgacc tacgtcctcg cgctgcagga ggccgtggcc gtgggcatgg
     6601 ccgacggctg ggcgcaggcg agcggcagga cagccttcgt gaacctgcac accacgccag
     6661 gcgtgggcca cgcgatgggc agcatcgtga acgcgcgcga caaccgcgcg ccgatggtcg
     6721 tcacggcggg gcagcaatcg cggccgatga tggctctgag cgcgctgctc acgaaccagg
     6781 acgcgaccac gctccccaag cccgccgtca agtggagcta cgagccggcg cgggccgagg
     6841 acgtgccgat ggccgtggcg cgcgggctcc agctg
//
LOCUS       DASVPL010000247        12022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_341363, whole genome shotgun sequence.
ACCESSION   DASVPL010000247 DASVPL010000000
VERSION     DASVPL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12022
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341363"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1996)
                     /locus_tag="VF517_13350"
     CDS             complement(176..1996)
                     /locus_tag="VF517_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8053970.1"
                     /translation="MESGTVAEQYETKFPALRETTLCGAFQATARECAERDAVRTKGG
                     EFSITWSEYAERVERTAAGLAGLGIGRGDTVAIMLTNRPEFHWVDAAAMHIGATPFSV
                     YNTYSSEQIEYLVSDAQNKIVITEQAFLDRVEKVKEACSAVEHVVVVDGDPGEHLSLE
                     DLEGRAEEGFDFEATWRAVDEDDVLTLIYTSGTTGPPKGVQITHGNEMTALRAFDQII
                     EFSAGAQLVSYLPMAHIAERSCTQYLPMGFGFTVTDCPDAREVISYLPEVRPSWFFAV
                     PRIYEKLKAGLEANLAKVEDEEQRAGAQKALEIGLKKVRLEQSGEEVPEELQRAYEQA
                     DEQLFSKLRKELGLDRLEAMNVGAAPTPREVIEFFHAIGLPLAELWGMSETCGAGTCN
                     PPGAVKIGTVGPASPFCEVKLAEDGEVLIKGGIVMKGYRNQPDKTKETFTDDGWLMTG
                     DIGEFDDDGYLKIVDRKKELIINAAGKNMSPANIEAKLKTAGPLIGQAVTIGDQRSYN
                     TALVTLDPDSLPGWAKSNGLEGRSIEELAEDPKVFEAVEAEVEKANESLARVEQIKKF
                     KILPTDWEPGGDELTPTMKLKRRPIGEKYAEEIEELYAGA"
     gene            complement(2031..3158)
                     /locus_tag="VF517_13355"
     CDS             complement(2031..3158)
                     /locus_tag="VF517_13355"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX8053971.1"
                     /translation="MSWFLAFLGFALLIMLHEAGHFAAAKAVGMRVERFSLFFPPFIW
                     RKQGKGETEYTIGVIPLGGYVKISGMNPDAELPPEVRDRAYHAQPVWKRIVVIAAGPL
                     VNLIIAIVLLFVFYWQVGELNHAQVDRIDKSYPAAQLLEPGDRVVSVDGMPVNIDTPE
                     GREKITTQISSHTCGQDRPVKGCRAKDPVNMVIERDGRTMPVAVTPIYDPDAEPVKEG
                     GQKGRMRIGFGYGQTGPRETMPAGKAFTTGIDRFWFVTVETVKLPARLFDAEKRKEVG
                     SVVGGYEVTRQTILSRVGDVVVVMAVISLSLAIINLFPFLPLDGGHIFWAIVEKIRRK
                     PVPLAIMERASVIGFLLVIGVFILGLSNDIDRLSGEGFQVR"
     gene            complement(3155..4156)
                     /locus_tag="VF517_13360"
     CDS             complement(3155..4156)
                     /locus_tag="VF517_13360"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX8053972.1"
                     /translation="MRRIVILGSTGSIGRQALDVIEASGGELEVVALAARRSSDALVE
                     QARAHGVTRIALADEHAAARASEAWTGGEVLAGAEGLVRLITETDCDLVLNALAGAAG
                     VGPSVAALGEGIDLALANRESLVVAGEIVMALAEATSTSIIPVHPDHSGLHQLISGER
                     PGTVDRLVLTAPPGGSLMDTGFDVIAATHLFGVPADDVEVLLHPERVVHALVQLNDGA
                     SLAHLGYPDMRVPISYALNHPERVDTPVEPLDLAALRRLTFEAPDLDAYPCLRLAYEA
                     ARAGGTAPCVLQAAYDVAPRAEVIERTLAAVEVTPVRHFSDLYRADEQARELAAVAA"
     gene            complement(4190..5272)
                     /locus_tag="VF517_13365"
     CDS             complement(4190..5272)
                     /locus_tag="VF517_13365"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8053973.1"
                     /translation="MAGLVFFTGFPGFIGTRVVRRLLADDPEVRVAALVESKMGARAR
                     EAAGRLEGGDRVEILEGDIADKSLGLTGEQRERLTAEVTVAYHLAAIYDLAVPSDIAQ
                     KVNVDGTGNVLDLLADCERLERHNYVSTAYVAGDRSGHVYEHELVMGQGFKNHYESTK
                     FQAEVWVRHSMDRIPTTIYRPAIVVGDSQTGETQKFDGPYYILRTISRALRMKSPIPL
                     FGKAGAPFNVVPVDFVVDALAAGASDPGSVGETLHLVDPDPITAAELARMLSREYAGK
                     EPGYKLPPKAVELSLHSKTVRKAFHGAPAQSIVYLNHPVSFDTRRAEALLARVGVRCP
                     RFEEYVGPIVSYFRQHEGDPAYAPAA"
     gene            complement(5355..6755)
                     /locus_tag="VF517_13370"
     CDS             complement(5355..6755)
                     /locus_tag="VF517_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053974.1"
                     /translation="MARTALVVLLFLAIATPAHAGGTVFSHPSPELYGDAVLAGERVV
                     WVTRSESPDGIEFHRARVDGSDHAITHVAAPVNPSGGNRAFFAFDASARRLAAGLHLL
                     RCTDCKYQSYESQFSRTLSGSLDGPLDEIARCSEGGGANPLPVDPRIAVSGDVVAVRS
                     PCRTQITVYDLATSPPQTREYPGAFFFRVAGDFLASQDGDGVVVRNWRSGEEVLRLPD
                     AAGFDLQPDGKLAFVARSGEQRPVMWASPASPVPREVAPHSAGATRIANDRIAHGRPG
                     GGVTVRTLGGDVLADSAVARLGDFDGGQVAWAARVCTRVVVATWDLAGEPPVTPAGDC
                     PLAALRSTTLRAWFAAVSAEHWATVKIVCPPDPPLGCEGSLALLAPDGRPRPKRRGLI
                     DFARGSYRLEPGERATVRLDFVPGGLCAVRRNALRPIFHAGGHPLYAPPGARAKRRRV
                     ALVGVRSYVDRCEPRT"
     gene            complement(6779..7573)
                     /locus_tag="VF517_13375"
     CDS             complement(6779..7573)
                     /locus_tag="VF517_13375"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX8053975.1"
                     /translation="MPETGKRFVQAVPLILFACLIVGYGGEVFMLGAAALGILMLNEL
                     YRMMGRARPVNLAGFLTLVALCLVALYGEREQLLLVLVISFPVTFALAAARQRRENVS
                     WGIAATYLGVIWIGLGVAHAVMLRELDHGGALLLDVLIGTFISDTAAYFGGRTWGRRR
                     IAPRISPNKTLEGLISGIAGGTFAFWLFGLAYHHEWFNGTDRVLIGLAVACAAPVGDL
                     FESMLKRDLGVKDTGRAFGSHGGVLDRLDGALFTLPAAFYVALALL"
     gene            complement(7573..8235)
                     /gene="uppS"
                     /locus_tag="VF517_13380"
     CDS             complement(7573..8235)
                     /gene="uppS"
                     /locus_tag="VF517_13380"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX8053976.1"
                     /translation="MPARRVAIIMDGNARWAAGRGLPTLAGHKAGVDTLKQTVKDAVK
                     LGVEDLTVYAFSTENWTRPDDEVTGLMALFAERIESETPELDDEGVRIRYVGRREGLD
                     DDLIRRMEWSEAETADNERLRLFVAFNYGGRAEILDAAARYEGGGEQEFAKLLYAPEM
                     NEPDLLIRTSGEHRLSNFLLWQTAYSELVFTDTLWPDFDESALRAALDEYDARERRFG
                     AR"
     gene            complement(8243..8812)
                     /gene="frr"
                     /locus_tag="VF517_13385"
     CDS             complement(8243..8812)
                     /gene="frr"
                     /locus_tag="VF517_13385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX8053977.1"
                     /translation="MSEGIIGEFLEDAADRMKKSVESTRHEFGSVRTGRATPALLDRV
                     NVDYYGAVTPLKQLAGISATEARLLTVTPYDKSSIKAIEKAILESDVGLTPSNDGNVI
                     RLIIPELTEERRRDLVKVVRRIAEDGRVAIRNVRRDVMHDLRELKEAGEVGQDDERRA
                     EQELQKLTDERVSELDEALAVKEKDILEV"
     gene            complement(8809..9561)
                     /gene="pyrH"
                     /locus_tag="VF517_13390"
     CDS             complement(8809..9561)
                     /gene="pyrH"
                     /locus_tag="VF517_13390"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX8053978.1"
                     /translation="MPAPEQEDPAAGRKPVFSRILLKLSGEALMGPLEYGADKERIAA
                     IAAQIQQVSDSGVEVAVVVGGGNIYRGLKGAAEGMDRATGDYMGMLATVLNALALQDA
                     LEKRGAVTRVQSAIEISEVAEPYIRRRAMRHLEHGRVVIFAAGTGNPFFTTDTAAALR
                     ALEMHAEALLMAKNGVEGVYDADPRTSPDANFLPTITHRQAIEQGLEVMDSTALSLCM
                     DNSLPIHVFNMDDETNIERIVSGERVGTLVST"
     gene            complement(9564..10166)
                     /gene="tsf"
                     /locus_tag="VF517_13395"
     CDS             complement(9564..10166)
                     /gene="tsf"
                     /locus_tag="VF517_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX8053979.1"
                     /translation="MADFTAADVKALRDQTGAGMMDCKRALEESDGDIEKAIEVLRVK
                     GQAQAAKRGARTASEGVIQSYIHSNGKIGVLVEVDCETDFVARNDDFQAFARDIALHV
                     AAAAPLYVTEEEVPEDVKAAERRIFEEQASDKPENVRPKIAEGRLRKWLDEIVLMRQP
                     HVNGDKYEGRTIEEIRADTAAKTGENVVIRRFTRFAVGEE"
     gene            complement(10169..11266)
                     /gene="rpsB"
                     /locus_tag="VF517_13400"
     CDS             complement(10169..11266)
                     /gene="rpsB"
                     /locus_tag="VF517_13400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX8053980.1"
                     /translation="MAAQVGMRDLLEAGVHFGHQTRRWNPKMRRYIFGERDGIHIIDL
                     QQTERLLARAQEFAAGVAARGGTVLFVGTKKQARDSIQETAEAAGMPYIHLRWLGGLL
                     TNFQTISKRTKRLHDLLDWTENGTLELLPTRERIGAIHEREKLMHNLGGVRDMQRPPD
                     AIFIVDLKTEEIGVREAVRLKIPIIGLVDTNCDPGPVNYVIPGNDDAIRSCKAIIEAI
                     GTVVGENSARFREEEERARKEAEEKARKEQEERERREAEEKARLEAEEAAAREAAAQE
                     AAAAAATGDQHGSPAPSEGQHPERPGGREVREQMEQAGEKPTREALEQAERDKKAPLD
                     EPPKRPRGPEEADAKASEQREGAVRPDQEVS"
     gene            complement(11447..>12022)
                     /locus_tag="VF517_13405"
     CDS             complement(11447..>12022)
                     /locus_tag="VF517_13405"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8053981.1"
                     /translation="GDDPWRPGVVHRLDRDTSGLLIVAKDDATHRALQDMLRRREIER
                     EYLALVEGRPDARSGTIDAPIGRDRRDRTAHSTSTDRPRDAVTHFAVERALPRTTLLR
                     VRLETGRTHQIRVHMAAIGHPVCGDARYGGEGCGARLVLTRQFLHSARLRFTHPATGG
                     QVRCESKLPVDLLRALEAAAREPVSEGPDGD"
BASE COUNT         1634 a         4361 c         4160 g         1867 t
ORIGIN      
        1 cgagatcgtg cgggaggcgg tggcctcccg ccgcacgatc cgcgaggtgg cgctcgagaa
       61 gggcgtcgac gaggagacgc tcgaccgcgc gctcgacctc caggcgatgg cgcgcggcgt
      121 caagccgagc ccgaccgcct aggcgccgcc gagcccgccc ggcgcgacgc gcgggctacg
      181 cgcccgcgta gagctcctcg atctcctccg cgtacttctc gccgatcggc cggcgcttca
      241 gcttcatcgt gggcgtcagc tcgtcgccgc cgggctccca gtcggtgggc aggatcttga
      301 acttcttgat ctgctccacc cgcgcgagcg actcgttggc cttctcgacc tcggcctcga
      361 cggcctcgaa caccttcggg tcctcggcca gctcctcgat cgacctgccc tcgagcccgt
      421 tcgacttcgc ccagccgggc aggctgtcgg gatcgagcgt gacgagcgcc gtgttgtacg
      481 agcgctggtc gccgatcgtg acggcctggc cgatcagcgg gccggccgtc ttcagcttgg
      541 cctcgatgtt cgccggcgac atgttcttgc cggcggcgtt gatgatcagc tccttcttgc
      601 ggtcgacgat cttcaggtag ccgtcgtcgt cgaactcgcc gatgtcgccg gtcatcagcc
      661 agccgtcgtc ggtgaaggtc tccttggtct tgtcgggctg gttgcggtag cccttcatga
      721 cgatcccgcc cttgatcagc acctcgccgt cctcggcgag cttgacctcg cagaacggcg
      781 acgccggccc gacggtcccg atcttgaccg cccccggcgg gttgcaggta cccgcgccgc
      841 aggtctcgga cataccccag agctcggcca gcggcaggcc gatcgcgtgg aagaactcga
      901 tcacctcgcg aggcgtgggc gcagcgccga cgttcatcgc ctcgagccgg tcgagcccga
      961 gctccttgcg cagctttgag aagagctgct cgtcggcctg ctcgtacgcc cgctggagct
     1021 cctcggggac ctcctcgccg gactgctcga gccgtacctt cttcagcccg atctcgagtg
     1081 ccttctgcgc cccggcgcgc tgctcctcgt cctcgacctt cgcgagattg gcctcgagcc
     1141 cggccttcag cttctcgtag atccgcggga cggcgaagaa ccagctcggc cggacctccg
     1201 gcaggtacga gatcacctcg cgcgcgtccg ggcagtcggt gaccgtgaag ccgaagccca
     1261 tcggcaggta ctgcgtgcac gagcgctcgg cgatgtgcgc catcgggagg taggacacga
     1321 gctgcgcgcc cgcggagaac tcgatgatct ggtcgaacgc gcgcagcgcc gtcatctcgt
     1381 tcccgtgcgt gatctgcacg cccttcgggg ggccggtggt gccggacgtg tagatcagcg
     1441 tcagcacgtc gtcctcgtcg accgcgcgcc aggtcgcctc gaagtcgaag ccctcctcgg
     1501 cgcggccctc gaggtcctcg aggctcaggt gctcgccggg gtcgccgtcg accacgacca
     1561 cgtgctccac cgcggagcag gcctccttga ccttctcgac gcgatcgagg aacgcctgct
     1621 cggtgatgac gatcttgttc tgcgcatccg agaccaggta ctcgatctgc tcggacgagt
     1681 aggtgttgta gaccgagaac ggcgtggccc cgatgtgcat cgcggcggcg tcgacccagt
     1741 ggaactcggg acggttggtg agcatgatcg cgacggtgtc gccgcgcccg atgccgagac
     1801 ccgcgaggcc cgcggccgtg cgctcgacgc gctcggcgta ctcgctccag gtgatgctga
     1861 actcaccgcc cttggtgcgc acggcgtcgc gctcggcaca ttcgcgcgcc gtcgcctgga
     1921 acgctccgca cagcgtcgtc tcgcgcagcg ccgggaactt cgtctcgtac tgctcggcga
     1981 cggttccgct ctccatccgg ctcctccctc cgaaagtgga gatcgggagg ctaacgcacc
     2041 tggaagccct ctcccgacag cctgtcgatg tcgttcgaga ggccgaggat gaacactcct
     2101 atgacgagca ggaagccgat cacgctggcg cgttccatga tcgcgagcgg gaccggcttg
     2161 cgccggatct tctcgacgat cgcccagaag atgtggccgc cgtcgagcgg caggaacggg
     2221 aacaggttga tgatcgcgag cgacagcgag atcaccgcca tgacgaccac cacgtcgccg
     2281 acgcgcgaga ggatcgtctg gcgcgtgacc tcgtagccgc cgaccacgct gcccacctcc
     2341 ttgcgcttct cggcgtcgaa cagccgcgcg ggcagcttga cggtctcgac cgtgacgaac
     2401 cagaagcggt cgatgccggt ggtgaacgcc ttgccggcgg gcatggtctc gcgcggaccg
     2461 gtctggccgt agccgaagcc gatccgcatg cggcccttct gcccaccctc cttcacgggc
     2521 tcggcgtccg ggtcgtagat cggcgtgacc gcgaccggca tcgtgcgccc gtcgcgctcg
     2581 atcaccatgt tcaccggatc cttcgcgcgg cagcccttca cgggacggtc ctggccacac
     2641 gtgtgcgagc tgatctgggt cgtgatcttc tcgcggccct cgggggtgtc gatgttcacc
     2701 ggcatgccgt cgaccgacac gacgcggtcg cccggctcga gcagctgcgc ggccgggtag
     2761 ctcttgtcga tccggtccac ctgcgcgtgg ttgagctcgc ccacctgcca gtagaagacg
     2821 aacagcagga cgatcgcgat gatcaggttc acgagcggcc cggccgcgat caccacgatc
     2881 cgcttccaca ccggctgcgc gtggtaggcg cggtcgcgca cctccggcgg cagctccgcg
     2941 tccgggttca tcccgctgat cttcacgtag cccccgagcg ggatcacccc gatcgtgtac
     3001 tcggtctccc ccttgccctg cttgcgccag atgaatggcg ggaagaacag cgagaagcgc
     3061 tccacccgca tgccgacggc ctttgccgcg gcgaagtggc ccgcctcgtg cagcatgatc
     3121 agcagggcga agccgaggaa ggcgaggaac cagctcacgc ggccaccgcc gcaagctcgc
     3181 gggcctgctc gtcggctcgg tagaggtcgg agaagtgacg caccggagtg acctctacgg
     3241 ccgcgagcgt ccgctcgatc acctcggccc gcggggccac gtcgtaggcg gcctggagga
     3301 cgcacggcgc ggtccccccg gcgcgagctg cctcgtaggc aagtcgcagg cacgggtagg
     3361 cgtcgaggtc gggcgcctcg aaggtcagcc gccgcagcgc ggcgaggtcg aggggctcga
     3421 ccggggtgtc cacccgctcg gggtggttga gggcgtacga gatcggcacg cgcatgtcgg
     3481 ggtagccgag gtgagcgagg ctcgctccgt cgttgagctg caccagcgcg tgcaccaccc
     3541 gctcggggtg cagcagcacc tcgacgtcgt cggcgggcac gccgaacagg tgcgtggccg
     3601 cgatcacgtc gaagccggtg tccatcaggc tgccgccggg gggcgccgtg agcacgagcc
     3661 ggtccaccgt gccgggccgc tcgccggaga tgagctggtg gaggcccgag tggtcggggt
     3721 gaacggggat gatcgacgtg ctcgtggcct ccgcgagggc catgacgatc tcgccggcga
     3781 ccacgagact ctcgcggttg gcgagcgcga gatcgatccc ctcgcccagc gccgccacac
     3841 tggggcccac gcctgcggcg cccgcgagcg cgttcagcac gaggtcgcag tcggtctccg
     3901 tgatgagccg gacgagaccc tccgcacccg ctagcacctc gccgccggtc caggcctccg
     3961 aggcccgggc cgcggcgtgc tcgtcggcga gcgcgatgcg cgtgacgccg tgcgcccgcg
     4021 cctgctcgac cagcgcatcc gagctgcgcc gggccgcgag cgcgaccacc tcgagctcgc
     4081 cgccgctcgc ctcgattacg tcgagcgcct gccggccgat cgagccggtg gagccgagaa
     4141 tgacgattcg ccgcatcgcg gcgagtctag ggacgcgcct atgcggctct taagcggccg
     4201 gcgcgtacgc cgggtcgccc tcgtgctgcc tgaagtacga gacgatcggg ccgacgtact
     4261 cctcgaagcg cgggcagcgc acgccaacgc gggccagcag cgcctcggcg cgacgcgtgt
     4321 cgaagctcac cgggtggttg aggtagacga tcgactgcgc cggcgccccg tggaacgcct
     4381 tgcgcacggt cttgctgtga agggacagct cgaccgcctt cggggggagc ttgtagccgg
     4441 gctccttgcc cgcgtactca cgcgacagca tccgcgccag ctcggcggcg gtgatcgggt
     4501 cggggtcgac caggtgcagc gtctccccca cgctcccggg atccgaggcg ccggccgcca
     4561 gggcgtccac cacgaagtcg accggcacca cgttgaacgg cgcgcccgcc ttgccgaaca
     4621 gcgggatcgg cgacttcatc cgcagcgccc gcgagatcgt gcggaggatg tagtaggggc
     4681 cgtcgaactt ctgcgtctcg ccggtctgcg agtcgcccac gacgatcgcc gggcggtaga
     4741 tcgtggtcgg gatgcggtcc atcgagtggc ggacccagac ctcggcctgg aacttggtcg
     4801 actcgtagtg gttcttgaag ccctggccca tgacgagctc gtgctcgtag acgtggccgc
     4861 tgcggtcgcc cgccacgtac gccgtgctca cgtagttgtg gcgctcgagc cgctcgcagt
     4921 cggcgagcag gtcgagcacg ttgccggtgc cgtcgacgtt caccttctgc gcgatgtccg
     4981 acggcacggc gaggtcgtag atcgccgcga ggtggtacgc gacggtcacc tccgccgtca
     5041 gccgctcgcg ctgctcgccg gtgagaccga ggctcttatc ggcgatgtcg ccctcgagga
     5101 tctcgacgcg gtcgccgccc tcgagcctgc cggccgcctc gcgagcccgg gcgcccatct
     5161 tcgactccac gagggcggcc acccgcacct cggggtcgtc ggcgagcagg cgccggacga
     5221 cgcgcgtccc gatgaagccg ggaaacccgg tgaagaagac gagccctgcc atgcggcgcg
     5281 gcagtctacg cattcagggg cgagagtcca tttcttacgg ctataggcgt aagaaaagga
     5341 ctcgcacccc cgtttcaggt gcgcggctcg cagcgatcga cgtacgagcg cacacccacc
     5401 agagccaccc gccggcgctt cgcacgcgcc cccggcggcg cgtagagcgg gtgcccgccc
     5461 gcgtggaaga tcggccgcag cgcgttgcgg cggaccgcgc agaggccgcc ggggacgaag
     5521 tcgagccgga ccgtcgcgcg ctcgccgggc tcgagccggt aggagccgcg ggcaaagtcg
     5581 atcagccccc tgcgcttcgg gcgcggccgg ccgtcggggg ccagcagggc caggctgccc
     5641 tcgcacccca gcggcgggtc cggcgggcaa acgatcttca cggtcgccca gtgctcggcc
     5701 gagacggcgg cgaaccacgc gcggagcgtg gtgctgcgca gcgcggcgag cgggcagtcg
     5761 cccgccgggg tgaccggggg ctctccagcg aggtcccagg tggcgacgac cacgcgcgtg
     5821 cacacccgcg cggcccaggc cacctggccg ccgtcgaagt cgccgagccg cgcgacggct
     5881 gagtcggcca gcacgtcgcc gccgagcgtg cggaccgtga cgccgccgcc gggtcgcccg
     5941 tgcgcgatgc ggtcgttcgc gatccgcgtc gcaccggcgg agtgcggcgc gacctcccgc
     6001 ggcaccggcg acgccggcga cgcccacatc accggccgct gctccccgct ccgcgcgacg
     6061 aacgccagct tcccgtcggg ctggaggtcg aagccggcgg cgtcgggcag ccgcagcacc
     6121 tcctcgccgc tgcgccagtt gcggaccacc acgccgtcgc cgtcctggct cgccaggaag
     6181 tcgccggcga ccctgaagaa gaacgcgccc gggtactcgc gggtctgcgg cggcgaggtc
     6241 gcgaggtcgt acacggtgat ctgcgtgcgg cacgggctgc gcaccgccac gacgtccccg
     6301 ctcaccgcga tccgcgggtc caccggcagc gggttcgccc cgccgccctc cgagcagcgc
     6361 gcgatctcat ccagcgggcc gtcgagcgag ccgctcagcg tccgcgagaa ctgggactcg
     6421 tagctctggt acttgcagtc ggtgcagcgg agcaggtgga gcccggcagc gagccggcgc
     6481 gcggacgcgt cgaacgcgaa gaacgcccgg ttgccgccgc tcgggttcac cggcgccgcg
     6541 acgtgcgtga tcgcgtggtc gctgccatcg acgcgggctc tgtggaactc gatcccgtcc
     6601 ggcgactccg accgcgtcac ccagaccacg cgctcgcccg cgaggaccgc gtcgccgtac
     6661 agctcaggcg aggggtggga gaacaccgtc ccgcccgcgt gggccggtgt ggcgatcgcc
     6721 aggaacagca ggaccacgag cgcggtgcga gccacgccgc tagaacccgt gccggaaact
     6781 aaaggagcgc cagcgcgaca tagaacgccg ccggcagcgt gaacagcgcg ccgtcgagcc
     6841 ggtcgagcac gccgccgtgc gagccgaacg cgcggccggt gtccttcacg ccgaggtcgc
     6901 gcttgagcat cgactcgaac aggtcgccga ccggcgccgc gcacgccacg gcgaggccga
     6961 tcagcacccg gtcggtgccg ttgaaccact cgtggtggta cgcgagcccg aacagccaga
     7021 acgcgaacgt gccgccggcg atcccggaga tgagcccctc cagcgtcttg ttcggcgaga
     7081 tccgcggagc gatccggcgc cgcccccacg tgcgcccgcc gaagtaggcc gccgtgtcgg
     7141 agatgaacgt cccgatcagc acgtcgagca gcagcgcccc gccgtggtcg agctcgcgca
     7201 gcatgaccgc gtgcgccacg cccagcccga tccagatcac gccgaggtag gtcgccgcga
     7261 tcccccacga gacgttctcg cgccgctgcc gcgccgccgc cagggcgaac gtcacgggga
     7321 acgagatcac gagcacgagc aggagctgct cccgctcgcc gtacagcgcg acgaggcaca
     7381 gcgccaccag ggtcaggaac cccgccaggt tcacgggccg agcgcgcccc atcatccggt
     7441 agagctcgtt gagcatcagg atcccgaggg ccgccgcgcc gagcatgaag acctcgccgc
     7501 cgtagccgac gatcaggcac gcgaacagga tcagcgggac cgcctgcacg aagcgcttgc
     7561 ccgtctcggg catcagcgcg ccccgaagcg gcgctcgcgc gcgtcgtact cgtcgagcgc
     7621 cgctctgagc gcggactcgt cgaagtccgg ccacagcgtg tcggtgaaga ccagctccga
     7681 gtaggccgtc tgccagagca ggaagttcga cagccggtgc tcgccgctcg tgcggatcag
     7741 caggtccggc tcgttcatct cgggcgcgta caggagcttc gcgaactcct gctcgccgcc
     7801 cccctcgtag cgggccgccg cgtccaggat ctcggcgcgg ccgccgtagt tgaacgccac
     7861 gaacaggcgc agtcgctcgt tgtccgcggt ctccgcctcc gaccactcca tgcgccggat
     7921 cagatcgtcg tcgagcccct cgcggcgccc cacgtagcgg atccgcacgc cctcgtcgtc
     7981 gagctccggg gtctcggact cgatccgctc ggcgaacagc gccatcagcc ccgtcacctc
     8041 gtcgtcgggc cgtgtccagt tctcggtgga gaaggcgtag acggtgaggt cctccacccc
     8101 gagcttcacc gcgtccttga cggtctgctt cagcgtgtct acgccggcct tgtggcccgc
     8161 gagcgtcggc aacccgcgcc ccgcggccca gcgggcgttg ccgtccatga tgatcgcgac
     8221 gcgacgagcc ggcacgccgc catcagacct cgaggatgtc cttctccttg acggccaggg
     8281 cctcgtcgag ctccgacacg cgctcgtcgg tgagcttctg gagctcctgc tccgcgcggc
     8341 gctcgtcgtc ctgcccgacc tccccggcct ccttcagctc gcgcaggtcg tgcatcacgt
     8401 cgcggcgcac gttgcggatc gccacgcgcc cgtcctcggc gatgcggcgc accaccttca
     8461 ccaggtcgcg ccggcgctcc tcggtcagct cggggatgat cagccggatc acgttgccgt
     8521 cgttcgacgg cgtcaggccg acgtccgact cgaggatcgc cttctcgatg gccttgatcg
     8581 acgacttgtc gtacggggtc accgtgagca gccgggcctc ggtcgcagag attccggcga
     8641 gctgcttgag cggcgtgacg gcgccgtagt agtccacgtt cacgcggtcg aggagggcgg
     8701 gcgtggcgcg gccggtgcgg acgctcccga actcgtggcg cgtggactcg accgacttct
     8761 tcatgcggtc cgcggcgtcc tcgaggaact cgccgatgat cccttcgctc atgtcgaaac
     8821 cagcgtcccc actcgctcgc cggacactat ccgctcgatg ttggtctcgt catccatgtt
     8881 gaagacgtgg atcgggagcg aattgtccat gcacagcgac agcgcggtcg agtccatcac
     8941 ctcgagcccc tgctcgatcg cctggcggtg ggtgatcgtc gggaggaagt tcgcgtcggg
     9001 cgaggtgcgc gggtcggcgt cgtacacgcc ctccaccccg ttcttcgcca tcagcagcgc
     9061 ctcggcgtgc atctcgagcg cgcgcagcgc ggcggcggtg tcggtcgtga agaacgggtt
     9121 gccggtgccc gccgcgaaga tcacgacgcg gccgtgctcg aggtggcgca tcgcgcggcg
     9181 gcggatgtag ggctccgcga cctcggagat ctcgatcgcc gactgcacgc gcgtgacggc
     9241 gccgcgcttc tcgagcgcgt cctggagcgc cagcgcgttc agcacggtgg cgagcatccc
     9301 catgtagtcg ccggtggcgc ggtccatgcc ctccgcggcg cccttcaggc cacggtagat
     9361 gttcccgccg ccgaccacga ccgccacctc cacgccgctg tcggacacct gctggatctg
     9421 cgcggcgatg gccgcgatgc gctccttgtc ggcgccgtac tcgagtggcc ccatgagggc
     9481 ctcgcccgag agcttcagga ggatgcggct gaagaccggc ttccgcccgg cggccggatc
     9541 ctcctgctcg ggcgcgggga tcgctactcc tcgcccaccg cgaagcgggt gaagcggcgt
     9601 atcaccacgt tctcgccggt cttcgcggcg gtgtcggccc ggatctcctc gatcgtgcgg
     9661 ccctcgtact tgtcgccgtt cacgtgtggc tggcgcatca gcacgatctc gtcgagccac
     9721 ttgcgcaggc ggccctcggc gatcttcggc cgcacgttct cgggcttgtc cgaggcctgc
     9781 tcctcgaaga tgcgccgctc ggcggccttc acgtcctcgg ggacctcctc ctccgtgacg
     9841 tacagcggcg ccgccgcggc cacgtgcagg gcgatgtcgc gcgcgaacgc ctggaagtcg
     9901 tcgttgcggg cgacgaagtc ggtctcgcag tcgacctcga cgagcacgcc gatcttgccg
     9961 ttcgagtgga tgtagctctg gatcacgccc tccgaggcgg tgcgggcgcc gcgcttggcc
    10021 gcctgggcct ggcccttcac gcgcaggacc tcgatggcct tctcgatgtc gccgtcggac
    10081 tcctcgaggg cccgcttgca gtccatcatc cccgcgccgg tctgatcgcg cagggccttc
    10141 acgtcggccg cggtgaagtc agccatcgtc agctcacctc ctggtcgggc cgcacggccc
    10201 cctcgcgctg ctcggatgcc ttcgcatcgg cctcctcggg cccgcgcggg cgcttcggcg
    10261 gctcgtcgag cggggccttc ttgtcgcgct cggcctgctc gagcgcctcg cgcgtcggct
    10321 tctcgccggc ctgctccatc tgctcgcgca cctcgcggcc gccgggccgc tcggggtgct
    10381 ggccctcgct cggcgcgggc gagccgtgct ggtcgccggt ggcggcggcc gcggccgcct
    10441 cctgagcggc ggcctcgcgc gcggcggcct cctcggcctc caggcgcgcc ttctcctcgg
    10501 cctcgcggcg ctcgcgctcc tcctgctcct tgcgagcctt ctcctcggcc tccttgcgag
    10561 cgcgctcctc ctcctcgcgg aagcgggcgg agttctcgcc gacgaccgtg ccgatcgcct
    10621 cgatgatcgc cttgcacgag cggatcgcgt cgtcgttgcc ggggatcacg tagttcacgg
    10681 gccccgggtc gcagttggtg tcgacgagcc cgatgatcgg gatcttgagg cgcacggcct
    10741 cgcgcacacc gatctcctcg gtcttcaggt cgacgatgaa gatcgcgtcg ggcgggcgct
    10801 gcatgtcgcg cacgccgccg aggttgtgca tcagcttctc gcgctcgtgg atcgccccga
    10861 tgcgctcgcg cgtcggcagc agctcgagcg tgccgttctc ggtccagtcg agcaggtcgt
    10921 gcaggcgctt ggtccgcttc gagatcgtct ggaagttcgt gagcaggccg ccgagccagc
    10981 gcaggtggat gtacggcatg cccgccgcct cggcggtctc ctggatggag tcgcgggcct
    11041 gcttcttcgt gcccacgaac agcaccgtcc cgccgcgcgc cgcgaccccg gccgcgaact
    11101 cctgagcccg cgcgagcaga cgctcggtct gctgcaggtc gatgatgtgg attccgtccc
    11161 gctcgccgaa gatgtaacgg cgcatcttcg ggttccagcg cctcgtctgg tggccgaagt
    11221 ggaccccggc ctcgagcagg tctcgcatgc cgacctgtgc tgccacgact tctccttgtg
    11281 ttgtggtgcc cgccggcggg cgcgggaccc ggctcgaagc cgggcaggac cgcgtccgtt
    11341 tacccgtcgg ggtagacgac cgtgtgagcg ccggaggata gccgcagcct ggaaacggtc
    11401 ccggaatagg aacgggcgcc ccgaaggacg cccgtgccat ttctgttcag tccccgtctg
    11461 gcccttcgga gactggctcc cgtgcagctg cttcgagcgc gcgaagcagg tccacgggca
    11521 gtttggactc gcagcgcact tgtccccctg tcgccgggtg ggtaaacctc aatcgagccg
    11581 aatggagaaa ttggcgcgtt agcactagac gcgccccgca cccctcgcct ccgtagcggg
    11641 cgtcgccgca caccgggtgc ccgatcgcgg ccatgtgcac gcggatctgg tgggtgcggc
    11701 cggtctcgag ccgcacgcgg aggagcgtcg tgcgcggaag cgcgcgctcg accgcgaagt
    11761 gcgtgacggc atcgcgcggg cggtcggtgc tggtcgagtg cgccgtgcgg tcgcgccggt
    11821 cgcggccgat cggggcgtcg atggtgccgc tgcgggcgtc cgggcggccc tcgaccaggg
    11881 cgaggtactc gcgctcgatc tccctccgcc gcagcatgtc ctggagcgcg cggtgcgtcg
    11941 cgtcgtcctt ggccacgatc aggagcccgg acgtgtcgcg atcgagccgg tgcacgacgc
    12001 ccgggcgcca ggggtcgtcg cc
//
LOCUS       DASVPL010000248        13309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_185627, whole genome shotgun sequence.
ACCESSION   DASVPL010000248 DASVPL010000000
VERSION     DASVPL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13309
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185627"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VF517_13410"
     CDS             <1..619
                     /locus_tag="VF517_13410"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8053982.1"
                     /translation="MEAAQATERAERRLDSEKAKRIVAAMRASVARRGAAGSTFDHVA
                     QEAGVSRGLLHYYFGSKERLLVEVVRHDAWLRIQELEDRLRDADTLDAVIEALVVQLR
                     ELVEQDDVTHAVIHELLSAARQNDEIRDELGEHYRRLRGRVGEILREKEADGVVALRA
                     EPEAAAAVLLAIADGFEVQTTIDDDWDSAPTVAAAVAAARHLLGA"
     gene            747..3026
                     /locus_tag="VF517_13415"
     CDS             747..3026
                     /locus_tag="VF517_13415"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8053983.1"
                     /translation="MQRAMDKLSSFIERRRRLVVAIWVVALLAAMPFAARQTEHLTSG
                     GFTSPGSSSQKVDAALGSFPSVNREGLVAVLAMDRDASRKDLNAALARLRRAVDDHER
                     IEPLGGARSPRIQRADGGTRVALVPLRVEGSRNQAADAVVELRDDLLAEPQRKVEVQL
                     TGQQALWAGMQDVTKDDLESAETAGFPVVLVILLAVFGSLAAALLPLALGFAAVILTG
                     AEIFFLSQQTGMSVFVTNTASMIGIGVAVDYSLFVLARYREEIHGGATPEQARQTSMR
                     TSGIAVAFSGVTVLISLAGLFLVNSQTLRSMALGAIVVVAVSIVGAITLLPALMRMLG
                     PRAYSRGRIATVTGLLVRARRSGGRRRGRSDPELGGRTFWQRWTERVMRRPITAAVLS
                     AAVMLTLAFPTLSLDLGNGALDQFPEGNPTRVAAERAASIQGEGAAGPALVMARFRDG
                     SASDGRNRAAVEHFAAQMRRDRRVAAVSPPTVSRDRTAVLVAVTPRDHFESARADALL
                     ERLRSDSNPLTRVARVDVGGATGFNKDFIDLISGSMWKILLFVLAFSYLVLLVLLRSV
                     LLPLKAVLMNLLSVGAAYGVLVAIFQWGWADGFLGFQSLGYVQAMTPPFLLAVVFGLS
                     MDYEVFLLTRIRERYQATGDTQRAVAEGLAASARTITSAAAIMVAVFAVFAMTGVPSI
                     KEIGLGLSVAIALDATLVRLVLVPATMEIMGEWNWWLPKWLDRILPHTDFEASGEPPE
                     QPTPPAVPREQPAPAPAAG"
     gene            complement(3023..4309)
                     /locus_tag="VF517_13420"
     CDS             complement(3023..4309)
                     /locus_tag="VF517_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX8053984.1"
                     /translation="MAQAMADRHAQGLDAISYEDLYRRWEHGNWSAYDIDFSQDRVDW
                     SETFDDLERRSALWTYSMFFHGEDSVTDNLSPYIDAAPLEEQKYFLATQQVDEARHSV
                     FFNRFMQEVVNAGSGSVSNTLEATRPELSWGFRKTFAALDRIADELRRDRSIPNFARS
                     IVMYHFIVEATLAQPGQHFIESYCNERGILPGFASGVENVSRDEQRHIGFGVKCLSDL
                     NRQDPDCKAAVAELLRDVMPFASAVFVPPNWDRRYAEVFGKSLEDIYEDGMASFETKL
                     RMAGMPLESLPGAPPFPLDVTPRERAEQVVAILEAGYLGEKIGPPSKDPESVAQLFDL
                     IKRSIDKTAAPGGSATILWDFSDLDPWYLRIDNGSSDSRQGRPEDPDLTLRCRFEDWV
                     EIVARRRDPRVAIATGKLRPKGSMRVLLRMPKIFGR"
     gene            4369..5187
                     /locus_tag="VF517_13425"
     CDS             4369..5187
                     /locus_tag="VF517_13425"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8053985.1"
                     /translation="MSAAALAAIALAAAAPGGGAYEFARANAQAGPRPAASAAERRAH
                     IAVRERFADAGLGVEVQRFTVRGRGRSRNVIAKLDTPRTCLRIVMAHTDSTPNAPGAN
                     DNASGLGVLAELAPRLRALDPGCDVWLVATGAEERIYTGSPDHLGALALARLVRRRGL
                     QRRLRYALSLDEVGRDRPFWIRSPVGSPRGPVERELLAAARSERVGVSWVRDAGSGNS
                     DHREFELLGMRGMKLGVGADGEPCRHSPCDRASRLKQSSLRAALLVVERALRRR"
     gene            complement(5217..6566)
                     /locus_tag="VF517_13430"
     CDS             complement(5217..6566)
                     /locus_tag="VF517_13430"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX8053986.1"
                     /translation="MPDQVTMDKIVALCKRRGFIFQSSEIYGGLASTYDYGHYGVLLK
                     SNVKAEWWRAMVQERDDIVALDAAILMHPKTWEASGHLEGFTDPLVQCLGDCKRRWRE
                     DHLREAQPEGELHCPNCGGDLSEPRNFNLMFKTHMGPVEEEGNEIFLRPETAQGIFVN
                     FKNVLQFSRKRPPFGIAQVGKSFRNEITPGNFIFRTREFEQMEMEYFVPPTEADRFYD
                     FWREERRRWYEELGIRPDHLRLRDHDADELSHYSSATADVEYLFPIGWSELEGIANRG
                     NFDLTQHMTHSSEKLDYVDSATGDRYVPHVIEPAAGADRATLAFMVDAYDEEEVEGRE
                     RVLLRLHPRLAPVKVAVLPLLNKDGHPEVAREIYEDLRRRMPAEYDAGGSIGKRYRRQ
                     DEIGTPWGVTVDHQTIEDRTVTLRDRDSLDQSRIAIDELAGVLTERLAAPWQSPKLG"
     gene            6702..6839
                     /locus_tag="VF517_13435"
     CDS             6702..6839
                     /locus_tag="VF517_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053987.1"
                     /translation="MVSKAFRATSHVAALIVWHSMRVSILTDLDAPRPPALDGAASQA
                     A"
     gene            6872..7207
                     /locus_tag="VF517_13440"
     CDS             6872..7207
                     /locus_tag="VF517_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX8053988.1"
                     /translation="MPFYEYRCKNGHTFEVMQRMSDDPVSVCQECGAPVQRVFHPVAV
                     HFKGSGFYTTDYGRKKAGAGAGSDESKGSSSSSSDSNSSDSKGSESKSSDSKSSDSKS
                     EKSAAKKSD"
     gene            complement(7208..8335)
                     /locus_tag="VF517_13445"
     CDS             complement(7208..8335)
                     /locus_tag="VF517_13445"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX8053989.1"
                     /translation="MPDAPGGPPPPQGPPQPPPQPQRPPGPPQYKVYRSRRGLRDRLL
                     PDRAAGEDRVKPPRREGPPGLHPTKPRWRSALRYALIGAALWLLLSFVVFMVSAQTQD
                     GVSQRTENALAGGGSLLTGSTVLVLGSDQRPKGTKEPGAAGSPSRADSIMLLHVGVGS
                     VRKLSILRDSYANIPDSGPQRINAAYAIGGAALTIRTVQEFMGNGLEINHVIEVDFSN
                     FPELIDSLGGVDIELDNCLKSDRFGDKRVVLSKGKHHLTGREALRFARVRKNRCAPNE
                     DDRQRAARQQKVLAGMRARLVHPKNWPGSFIRAPWIAWEAPRALRTDMKGPGLLALFT
                     DLLTGGSGETTVLKPDGINGDGSLRIERSSRASAVRRLTGD"
     gene            8357..9304
                     /locus_tag="VF517_13450"
     CDS             8357..9304
                     /locus_tag="VF517_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8053990.1"
                     /translation="MRVIGIDAGGTKLLGGVVDDALSVRDRVHRFWRGETREQVLDVI
                     VGAVEEARAAAPDVGAVGIGIPSVVDAEAGVSEYSVHLPLEGVAVRDLMAERLGLPVW
                     IDNDTNLALLSEWRAGAARGVRHAVMLTLGTGIGGALLLDGRLYRGARGGGAELGHMV
                     VDVDGPPCQGGCPNRGCLEALASGTAIGREGAAAAAAAPDSGLGRAAAAGREVTGALV
                     CEVAHDGCTVAREVLALTGRRLGAGLASLVNAFQPEVLVIGGGAAAAGELLLGPAREV
                     AEATALPPLSAGVRIVAAELGAEAGLVGAGVLALEEGRV"
     gene            9301..10122
                     /gene="rsmI"
                     /locus_tag="VF517_13455"
     CDS             9301..10122
                     /gene="rsmI"
                     /locus_tag="VF517_13455"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX8053991.1"
                     /translation="MSAGRLVVCPTPIGNLEDVTLRVLSALREADVVACEDTRQTGKL
                     LERYGVSAPLVSYHEHNEEARSAQLVERMRAGETVALVSDAGMPLISDPGYVLVRACA
                     AAGLAVEVLPGPSAALAALVASALPADRWRFVGFLPRKAGRLREVLGEPGGTLVAFES
                     PRRLAATLAALAELDPERAAAVCRELTKVHEEVVRGTAAELAARYASEEPRGEIVLVV
                     AGAPEAPPGAAAAASVDAVARLVEAGAKARVAASVVAELTGEKANALYRGFLERG"
     gene            11685..13190
                     /gene="metG"
                     /locus_tag="VF517_13460"
     CDS             11685..13190
                     /gene="metG"
                     /locus_tag="VF517_13460"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX8053992.1"
                     /translation="MAYYVTTPIYYVNAQPHLGHAYTTIAADVLARHMRQRGDEVFFL
                     TGTDEHGEPVAQAAEREGVSPRELADRNAAKFLDLAARVNASNDFFIRTSDERHVKRV
                     QEVLQRVYDRGHVYEGVYEGWYCPRCADFKTDAELVEGNKCPIHLIELDREREDNYFF
                     KLSEFQVPLERMFEENPEWILPRSRYNEALSFIRGGLQDVSLTRGKITWGVPVPWDDG
                     HVFYVWFDALLNYYTALAFARDGEDLTDTFWPADVHLIGKDILKFHAVFWPALLMAAG
                     IELPRRESIHGYLLMDDHKMSKSLGNVIDPFQVADVYGVDALRYYLFAAVGFGQDGSI
                     SPADLEARYTSDLANEYGNLASRTVGMIGRFRDGVVPDATAPPELAGAFENHAERVSA
                     HLDRVELTHALSAIWELVRRVNQYVQETEPWKLAKDPDAADRLDAALYGIAEGLRVAS
                     VLVYPFMPESAERVLAALGQPELKLDHARIGAVPGGARVSELPPLFPRIEK"
BASE COUNT         1733 a         4742 c         4898 g         1936 t
ORIGIN      
        1 gatggaggct gctcaggcaa ccgagcgcgc ggagcggcgg ctcgactccg agaaggcgaa
       61 gcggatcgtg gccgcgatgc gggcgagcgt cgcgcggcgc ggcgccgccg ggtcgacgtt
      121 cgaccacgtc gcgcaggagg cgggcgtctc gcgcggcctg ctgcactact acttcggctc
      181 gaaggagcgc ctgctcgtgg aggtggtgcg ccacgacgcc tggttgcgga tccaggaact
      241 cgaggaccgc ctgcgcgacg ccgacacgct cgacgcggtg atcgaggcgc tggtggtgca
      301 gctccgcgag ctcgtggagc aggacgacgt cacccacgcc gtcatccacg agctgctgag
      361 cgccgcgcgc cagaacgacg agatccgcga cgagctcggc gagcactacc ggcgcctgcg
      421 cggccgcgtc ggcgagatcc tgcgcgagaa ggaggccgac ggcgtcgtcg cgctgcgcgc
      481 cgagccggag gccgccgccg cggtgctcct cgcgatcgcc gacggcttcg aggtccagac
      541 gaccatcgac gacgactggg acagcgcgcc gacggtcgcg gccgcggtcg ccgcggcgcg
      601 gcacctgctc ggcgcctgac gggccgcgcg gcgcctgacg gagcgcgtga gctagttcac
      661 acccgcgacc gggccgagca ggcgatgatt ggccggccag ccaagatcga cgcggggcag
      721 ccgcggaggg ggactcggag ggatcgatgc agcgtgcgat ggacaagctc tcgtcattca
      781 tcgagcggcg ccgccgcctg gtcgtggcga tctgggtcgt cgccctgctc gccgcgatgc
      841 cgttcgcggc caggcagacc gagcacctga cgagcggtgg cttcacgtcg ccgggctcga
      901 gctcgcagaa ggtggacgcc gcgctcggca gcttccccag cgtcaaccgc gagggcctcg
      961 tggccgtgct cgcgatggat cgcgacgcga gtcgcaagga cctgaacgcc gcgctcgcgc
     1021 ggttgcgtcg cgcagtcgac gaccacgagc ggatcgagcc gctgggcggc gcgcggtccc
     1081 cgcggatcca gcgcgcggac ggcggcaccc gcgtcgcgct cgtcccgctg cgcgtcgagg
     1141 gcagccgcaa ccaggccgcc gacgcagtgg tcgagctgcg cgacgacctc ctcgccgagc
     1201 cgcaacgcaa ggtcgaggtc cagctcaccg gccagcaggc gctgtgggcc ggcatgcagg
     1261 acgtgaccaa ggacgacctc gagtccgccg agaccgccgg cttcccggtc gtgctggtga
     1321 tcctgctcgc cgtgttcgga tccctggcgg cggcgctgct gccgctcgcc ctcggcttcg
     1381 ccgcggtgat cctcaccggc gccgagatct tcttcctctc ccagcagacg ggcatgtcgg
     1441 tcttcgtgac caacaccgcg tcgatgatcg gcatcggcgt ggccgtcgac tactcgctgt
     1501 tcgtgctcgc ccgctaccgc gaggagatac acggtggagc caccccggag caggcacggc
     1561 agacctcgat gcgcacctcc ggaatcgccg tcgcgttctc gggcgtgacc gtcctgatct
     1621 cgctcgcggg ccttttcctc gtgaactcgc agacgctccg ctcgatggcg ctcggcgcga
     1681 tcgtggtggt ggccgtctcg atcgtcgggg cgatcacgct gctgccggcg ctgatgcgga
     1741 tgctcggccc ccgcgcgtac tcgcgcggac ggatcgcgac cgtcaccggg ctgctcgtgc
     1801 gcgcgcgccg cagcggtgga cgccggcgcg gacgctcgga ccccgagctc ggcggccgca
     1861 ccttctggca gcgctggacg gagcgcgtca tgcggcgccc gatcactgcg gcggtcctca
     1921 gcgcggccgt gatgctcacg ctcgccttcc cgacgctgtc gctcgacctg ggcaacggcg
     1981 cgctcgacca gttccccgag ggcaacccga cgcgcgtggc cgccgagcgc gcggcctcga
     2041 tccagggcga gggcgccgcg ggcccggcgc tcgtgatggc ccgcttccgc gacgggagcg
     2101 cgagcgacgg gcgcaaccgg gcggcggtgg agcacttcgc cgcgcagatg cggcgcgacc
     2161 gccgcgtcgc cgcggtcagc ccgccgacgg tctcgcgcga tcgcaccgcg gtgctggtcg
     2221 cggtcacccc gcgcgatcac ttcgagagcg cccgcgccga cgccctgctg gaacggctcc
     2281 gctcggattc caacccgctc acgcgcgtcg cgcgcgtgga cgtcggcggc gcgaccggct
     2341 tcaacaagga cttcatcgac ctgatctccg gctcgatgtg gaagatcctc ctgttcgtcc
     2401 tcgcgttcag ctacctcgtg ctgctcgtgc tgctgcgctc ggtgctgctg cccctgaagg
     2461 ccgtgctgat gaacctgctc tcggtcggcg ccgcgtacgg cgtgctcgtt gcgatcttcc
     2521 agtggggctg ggccgacggc ttcctcgggt tccagagcct cggctacgtg caggcgatga
     2581 cgccgccgtt cctgctcgcc gtggtcttcg gcctgtcgat ggactacgag gtgttcctgc
     2641 tcacacggat ccgcgagcgc taccaggcca ccggcgacac ccagcgcgcc gtcgccgagg
     2701 gcctcgcggc cagcgcgcgg acgatcacga gcgcggccgc gatcatggtc gccgtcttcg
     2761 cggtgttcgc gatgacgggc gtgccgtcga tcaaggagat cggactgggg ctgtcggtgg
     2821 cgatcgcgct ggacgcgacg ctcgtccggc tcgtgctcgt ccccgccacc atggagatca
     2881 tgggcgagtg gaactggtgg ctgccgaagt ggctcgaccg gatcctcccg cacacggact
     2941 tcgaggcgag cggcgagccg cccgagcagc ccacgcctcc ggccgtgccg cgcgagcagc
     3001 cggcgcccgc gccggcagcc ggctagcgcc cgaagatctt cggcatccgc aggagcacgc
     3061 gcatgctgcc cttcggccgg agcttgccgg tcgcgatggc gacgcgcggg tcacgcctgc
     3121 gggccacgat ctcgacccag tcctcgaacc ggcagcgcag cgtcaggtcc gggtcctcgg
     3181 gcctgccctg ccgggagtcc gagctgccgt tgtcgatgcg caggtaccac gggtcgaggt
     3241 ccgagaagtc ccacaggatc gtcgcggagc cgcccggcgc ggcggtcttg tcgatcgagc
     3301 gcttgatcag gtcgaacagc tgcgccacgc tctccgggtc cttgctcggc gggccgatct
     3361 tctcgccgag gtagccggcc tcgagtatcg ccaccacctg ctcggcgcgc tcgcgcgggg
     3421 tcacgtcgag tgggaacggc ggcgcgcccg gcaggctctc gagcggcatc cccgccatgc
     3481 gcagcttcgt ctcgaacgac gccatcccgt cctcgtagat gtcctcgagc gacttgccga
     3541 agacctccgc gtagcggcgg tcccagttcg gcgggacgaa cacggccgag gcgaacggca
     3601 tgacgtcgcg cagcagctcg gcgacggcgg ccttgcagtc gggatcctgc ctgttgaggt
     3661 cggacaggca cttcacgccg aagccgatgt ggcgctgctc gtcgcgcgag acgttctcca
     3721 cgccgctggc gaagccgggc aggatgccgc gctcgttgca gtagctctcg atgaagtgct
     3781 gcccgggctg cgcgagggtg gcctcgacga tgaagtggta catcacgatc gagcgcgcga
     3841 agttcggtat cgagcggtcg cggcgcagtt cgtcggcgat cctgtcgagc gccgcgaagg
     3901 tcttgcggaa gccccagctc agctccggcc gggtggcttc gagcgtgttc gagacggatc
     3961 cactgccggc gttgaccacc tcctgcatga agcggttgaa gaagacggag tggcgcgcct
     4021 cgtcgacctg ctgggtcgcg aggaagtact tctgctcctc gagcggggcg gcgtcgatgt
     4081 agggggagag gttgtcggtg acggagtcct cgccgtggaa gaacatcgag tacgtccaga
     4141 gtgcggaccg gcgctcgagg tcgtcgaacg tctcgctcca gtcgacgcgg tcctggctga
     4201 agtcgatgtc gtaggcgctc cagttgccgt gctcccagcg cctgtagaga tcctcgtacg
     4261 agatcgcgtc gagaccctgt gcgtgccggt ccgccatcgc ctgtgccatg tgtcgtgctc
     4321 tcccccgtcg ccctggccga tgagtgagtt tatgccagga tcggctcgat gagcgctgcc
     4381 gccctagccg cgatcgccct ggccgcggcg gctcccggtg gcggagcgta cgagttcgca
     4441 cgcgccaacg cccaggcggg cccgcgtccg gccgcctccg ccgccgagcg gcgcgcgcac
     4501 atcgcggtgc gtgagcggtt cgccgacgcg gggctcggtg tcgaggtgca gcgcttcacc
     4561 gtgcgcggcc gcggccgctc gcgcaacgtg atcgcgaagc tcgacacgcc gcgcacgtgc
     4621 ctgcgcatcg tcatggcgca caccgacagc acgccaaacg cgccgggcgc gaacgacaac
     4681 gcgtcgggcc tcggcgtgct cgcggagctc gcgccgcggc tgcgcgcgct cgaccccggc
     4741 tgcgacgtgt ggctggtcgc caccggcgcc gaggagcgga tctacaccgg ctccccggac
     4801 cacctcgggg cgctcgcgct cgcgcggctc gtgcgccgcc gcggcctcca gcggcggctg
     4861 cgctacgcgc tgtcgctcga cgaggtcggg cgcgaccggc cgttctggat ccgctcgccc
     4921 gtgggctcgc cgcgcgggcc ggtcgagcgg gagctgctgg ccgccgcgcg cagcgagcgc
     4981 gtgggggtga gctgggtgcg cgacgcgggc agcggcaact ccgaccaccg cgagttcgag
     5041 ctgctcggga tgcgcggcat gaagctgggc gtgggggccg acggcgagcc gtgccgccac
     5101 agcccgtgcg accgcgcgag ccgcctgaag cagtcgtcgc tgcgcgcggc gctgctggtg
     5161 gtcgagcgcg cgctgcgccg gcgctgagcg ggggtccggg ccccagcgtg gcggcgctaa
     5221 ccgagcttcg ggctctgcca gggggcggcg agccgctcgg tgagcacgcc cgccagctcg
     5281 tcgatcgcga tgcgcgactg gtcgagcgaa tcgcggtcgc gcagcgtgac cgtccggtcc
     5341 tcgatcgtct gatggtcgac ggtcacgccc cacggcgtgc cgatctcgtc ctggcgccgg
     5401 tagcgcttcc cgatcgagcc gcccgcgtcg tactcggccg gcatccgccg acgcaggtcc
     5461 tcgtatatct cgcgcgcgac ctcgggatga ccgtccttgt tgagcagcgg cagcaccgcg
     5521 accttcaccg gcgcgagccg cgggtgcagg cgcagcagca cgcgctcacg cccctcgacc
     5581 tcctcctcgt cgtaggcgtc gaccatgaac gcgagcgtcg cgcggtcggc gcccgcggcg
     5641 ggctcgatca cgtgcgggac gtagcggtcg ccggtggcgg agtcgacgta gtcgagcttc
     5701 tcgctcgagt gcgtcatgtg ctgggtgagg tcgaagttgc cgcggttggc gatgccctcg
     5761 agctcggacc agccgatcgg gaagaggtac tccacgtcgg cggtcgcgct cgagtagtgc
     5821 gagagctcgt cggcgtcgtg gtcgcggagg cgcaggtggt cgggccggat gccgagctcc
     5881 tcgtaccagc ggcggcgctc ctcgcgccag aagtcgtaga agcggtcggc ctcggtgggc
     5941 gggacgaagt actccatctc catctgctcg aactcgcgcg tgcggaagat gaagttgccc
     6001 ggggtgatct cgttgcggaa cgacttgccg acctgcgcga tgccgaacgg cgggcgcttg
     6061 cgcgagaact ggaggacgtt cttgaagttg acgaagatgc cctgcgcggt ctccgggcgc
     6121 aggaagatct cgttgccctc ctcctccacc ggccccatgt gggtcttgaa catgaggttg
     6181 aagttgcgcg gctcggagag atctccgccg cagttcgggc agtggagctc gccctcgggc
     6241 tgagcctcgc ggaggtggtc ctcacgccag cggcgcttgc agtcgccgag gcactggacg
     6301 agcgggtcgg tgaacccctc caggtggccg gacgcctccc acgtcttggg gtgcatgagg
     6361 atggcggcgt cgagggccac gatgtcgtcg cgctcctgga ccatcgcccg ccaccactcg
     6421 gccttgacgt tgctcttcag caggacgccg tagtggccat agtcgtaggt cgaggcgagc
     6481 ccgccgtaga tctcggacga ctggaagatg aacccacgcc gcttgcagag ggcgacgatc
     6541 ttgtccatgg tcacctggtc gggcacggcg cggaacccta gaggacgccc cggagcccgc
     6601 gatctcggcg cactgacgct cgcactttcc ccgctaatag cgtgtttctg gagaggacgg
     6661 ccgccgggta gctcctgtac tgtgccgcgc ggtctccgcc catggtgagc aaagccttca
     6721 gagcaacgag ccacgtcgcc gcactgatcg tgtggcactc gatgcgcgtc tcgatcctca
     6781 cggacctgga cgcgccccga ccgcccgcgc tggacggcgc cgccagccag gccgcctaac
     6841 cggcgccgct cgcgtcaata gactcgggcg catgccgttc tacgagtacc gctgcaagaa
     6901 cgggcacacc ttcgaggtca tgcagcggat gagcgacgac ccggtttcgg tgtgccagga
     6961 gtgcggcgcg ccggtgcagc gggtcttcca cccggtcgcg gtccatttca agggatcggg
     7021 cttctacacg accgactacg gccgcaagaa ggccggcgcg ggggcggggt cggacgagtc
     7081 gaagggctcc tcgtcctcct cctccgactc gaattcgtcg gactccaagg ggtcggagtc
     7141 gaagtcctcg gactcgaagt cctcggattc gaagtccgag aagtccgccg cgaagaagtc
     7201 cgactagcta gtcgccggtg aggcgccgga ccgcggacgc gcggctcgag cgctcgatgc
     7261 ggaggctgcc gtcgccgttg atgccgtcgg gcttcagcac ggtcgtctcg cccgagccgc
     7321 cggtcagcag gtccgtgaag agggcgagca ggccgggccc cttcatgtcg gtgcggagtg
     7381 cccgcggcgc ctcccacgcg atccagggcg cgcggatgaa gctccccggc cagttcttcg
     7441 gatggacgag ccgcgcgcgc atgccggcga ggaccttctg ctgtcgcgcc gcgcgctggc
     7501 ggtcgtcctc gttcggagcg cagcggttct tcctcacgcg cgcgaagcgg agcgcctcgc
     7561 ggccggtcag gtggtgcttg cccttgctga gcacgacgcg cttgtcgccg aagcggtcgg
     7621 acttgaggca gttgtcgagc tcgatgtcga cgccgccgag ggagtcgatc agctccggga
     7681 agttcgagaa gtcgacctcg atgacatggt tgatctcgag cccgttgccc atgaactcct
     7741 ggacggtgcg gatggtgagc gcggcgccgc cgatcgcgta ggcggcgttg atccgctgcg
     7801 gtcccgagtc cgggatgttc gcgtaggagt cgcgcaggat cgagagcttg cggacgctgc
     7861 cgacgcccac gtgcagcagc atgatgctgt ccgcgcgcga gggcgagccc gccgcaccgg
     7921 gctccttggt ccccttcggc cgctggtcgg acccgagcac gagcacggtg ctcccggtga
     7981 gcaggctgcc gccgcccgcg agcgcgttct cggtgcgctg cgatacgccg tcctgggtct
     8041 gcgcgctcac catgaagacg acgaatgaga gcagcagcca gagggcggcg cctatgagcg
     8101 cgtagcgcag cgcgctgcgc cagcgcggct tggtggggtg gaggcccggg gggccctccc
     8161 ggcgcggggg cttgacgcgg tcctcgccgg cggcccggtc ggggagcagg cggtcgcgca
     8221 ggccgcgccg cgagcggtag accttgtact ggggcgggcc gggcggacgc tgcggctgtg
     8281 gcggcggctg cggcggtccc tggggcggcg gggggccgcc gggcgcgtcg ggcatggccc
     8341 ttgtatacaa gctgcagtgc gcgtgatcgg gatcgacgcc ggtgggacga agctactcgg
     8401 tggtgtggtc gacgacgccc tgtcggtccg cgaccgcgtc catcgcttct ggcggggcga
     8461 gacccgtgag caggtcctcg acgtgatcgt cggcgccgtc gaggaggcgc gcgcggcggc
     8521 gccggacgtg ggcgcggtcg gcatcgggat cccctcggtg gtcgacgctg aggccggcgt
     8581 gtcggagtac agcgtgcacc tcccgctcga gggagtggcg gtccgcgacc tgatggcgga
     8641 gcggctcggg ctgccggtgt ggatcgacaa cgacacgaac ctcgcgctgc tcagcgagtg
     8701 gcgggcgggc gccgcgcgcg gtgtgcgcca cgcggtgatg ctcacgctcg gcaccgggat
     8761 cggcggtgcc ctgctgctcg acgggcggct ctaccgcggc gctcgcggcg gcggggccga
     8821 gctcggccac atggtcgtgg acgtagacgg gccgccgtgc cagggcggct gcccgaaccg
     8881 cggctgcctg gaggcgctgg cgtcgggcac ggcgatcggg cgcgaggggg ccgcggcggc
     8941 ggctgctgct ccggactcgg ggttggggcg cgcggccgcc gccgggcgcg aggtgacggg
     9001 cgcgctcgtg tgcgaggtcg cgcatgacgg ctgcacggtc gcgcgcgagg tgctggcact
     9061 gaccggccgc cggctgggag cggggctcgc ctcgctcgtg aacgcgttcc agccggaggt
     9121 gctggtgatc ggcggtggcg cggcggcggc gggtgagctg ctgctggggc cggcgcgtga
     9181 ggtcgcggag gcgacggcgc tgccgccgct ttcggccggg gtccggatcg tggcggcgga
     9241 gctgggcgcg gaggccgggc tcgtgggcgc cggggtgctg gcgctcgagg aggggcgggt
     9301 gtgagcgcgg ggcggctcgt cgtgtgcccg acgccgatcg ggaatttgga ggacgtgacg
     9361 ctgcgggtgc tgtcggcgct gcgcgaggcg gacgtggtcg cctgcgagga cacgcggcag
     9421 acggggaagc tgctcgagcg ctacggggtg agtgcgccgc tcgtctccta tcacgagcac
     9481 aacgaggagg cgcgctccgc gcagctcgtg gagcggatgc gggcgggcga gaccgtggcg
     9541 ctcgtctccg atgccggcat gccgctgatc tccgacccgg gctacgtgct cgtgcgagcg
     9601 tgtgcggcgg cggggctggc ggtcgaggtg ctgccggggc cgtcggcggc gctggccgcg
     9661 ctcgtggcgt cggcgctgcc cgcggatcgc tggcgcttcg tggggttcct gccgcgcaag
     9721 gcgggccggt tgcgcgaggt gctgggcgag ccgggcggga cgctggtggc cttcgagtcg
     9781 ccgcggcggc tggccgcgac gctggcggcg ctggcggagc tcgacccgga gcgcgccgcc
     9841 gccgtctgcc gggagctgac gaaggtgcac gaggaggtcg tgcgcgggac cgcggcggag
     9901 ctcgcggcgc gctacgcgtc ggaggagccg cgcggcgaga tcgtgctggt ggtggcgggc
     9961 gccccggagg cgccgccggg cgccgcggcg gccgcgtcgg tggacgctgt ggcgcggctc
    10021 gtggaggcgg gggcgaaggc gcgggtggcg gcgagcgtgg tggcggagct cacgggggag
    10081 aaggcgaacg cgctgtatcg ggggttcttg gagcgggggt aggggttccg gccgcgcccg
    10141 agctcccgcc gcgccccgcc gcgcccgctg cgccttcccc gccgcgcccg cccctgtcgc
    10201 cgcgctcgct ttgcccgccg cggccttccc cgccgcacct tgcgcgccgc gcccgacaac
    10261 caccttcgtg ccggaccgag cgaggttcgc cgacattcct gccctgaggg tccatatgac
    10321 ccataagtca ggaacgtcga gaaatccgcc cgttccccgc gccggttcgc ccctcagagc
    10381 gtgtcattcg cgtgccgatc cgtgtcgatc tcgcgccgac tgttacagct ccgcaacggt
    10441 gtgcaaaagg agtgaatcgc cctcgcgaag ccacgccgga acccgcttac ggtcgccgtc
    10501 atgagaactc gcatgccatc actgctcgcc ctcattgccc tcgccctgat cgcgctcgcg
    10561 ccgccggccg cggcgcgcgg tagcgggtgg cgatggccgg tcgacggacc ggtggtgact
    10621 cagtaccgca acggtgagga cccgtacgcg ggtggccagc accggggaat cgacgtctcc
    10681 gcggccgccg gcacgccggt cgtcgccgcg accgacggcg acgtccgctt cgcggggcca
    10741 ctcggcagct ccgggctgac cgtctcgatc cgcacggccg acggcagcta cgacacctcg
    10801 tacctccacc tcggctcgat cgaggtgcgt gcaggcgacc gggtcggagt cggcgcgcgg
    10861 atcggaagcg tcggcaccac tggcagccgc acgctgtccg agccgcacct ccacttcggc
    10921 gtccgcgagg ccggcgaccg cttcgcgtac cgcgaccccc tcacgctcct gccgccaccc
    10981 ccgagcgggc cacgcgtgcc aacgccgcgc ccgctgccgc ttcccggccc cgcgcccgcg
    11041 cctccgctcc tgggcccgcc cgccgcggca gtggcagcgg cagcggctcc ggccccgccg
    11101 ctacccatcc catcgccggg acctgtcgcc gcacccgcgc cgctacccat cccatcgccg
    11161 ggaccggtcg ccgcacccgc gccgctgcac atcacgcacg ccgcatcgcc cccgcaatcc
    11221 accccgcacc gcgccgggtc gagccgaggg cttcccagtc cacattcagc cgcggccgcc
    11281 tctcccatcg actccgcacc aacacgcctg cgcgcggccg cgaaccccgg gcgggccggg
    11341 ctgtccaaca cggcgcaggg cgcccgcggc tccgaccggg ccacccacgg gccgcaaacc
    11401 gccgccacac gcgcggtgac cagcggcccc gcctcggccc caccgaaccc accacaccgg
    11461 acagcacacg cggcagccgc gccaccccgc gccgcgcgcc gcgaagaaac cggcctggac
    11521 ctcggctggc tcgccgcgtg cgccggcctc gtcgctgtcg cggcgctgct cgcacgaccg
    11581 cgcggcccgg ccgaatcgct gcgtcgcggg acccgcctcg ccgaccgcac gcgaccgcga
    11641 gctcgcgccg ctgctgccgg agggggcgcg gatagcatcg ccacgtggcc tactacgtca
    11701 ccacgccgat ctactacgtg aacgcccagc cgcatctcgg gcacgcgtac acgacgatcg
    11761 cggcggacgt gctggcgcgt cacatgcgcc agcggggcga tgaggtcttc ttcctcaccg
    11821 gcaccgacga gcacggcgag ccggtggccc aggcggccga gcgcgagggc gtgtccccgc
    11881 gcgagctggc cgaccgcaac gcggccaagt tccttgacct cgccgcgcgt gtcaacgcgt
    11941 cgaacgactt cttcatccgc acgtcggacg agcgccacgt gaagcgcgtg caggaggtgc
    12001 tgcagcgcgt gtacgaccgc ggccacgtct acgagggcgt ctacgagggc tggtactgcc
    12061 cgcgctgcgc ggacttcaag accgacgcgg agttggtgga gggcaacaag tgccccatcc
    12121 acctgatcga gctggaccgc gagcgcgagg acaactactt cttcaagctc tccgagttcc
    12181 aggtgccgct cgagcggatg ttcgaggaga accccgagtg gatcctgccg cgctcgcgct
    12241 acaacgaggc gctgtcgttc atccgcggcg gcctccagga cgtctccctc acgcgcggga
    12301 aaatcacatg gggcgtaccc gtcccgtggg acgacggcca cgtcttctac gtctggttcg
    12361 acgcgctcct caactactac accgccctcg ccttcgcgcg cgacggcgag gacctcaccg
    12421 acaccttctg gccggcggac gtccacctga tcggcaagga catcctcaag ttccacgccg
    12481 tgttctggcc ggccctgctc atggcggcgg gcatcgagct gccgcggcgc gagtccatcc
    12541 acggctacct cctgatggac gaccacaaga tgtcgaagtc gctcggcaac gtgatcgatc
    12601 cgttccaggt cgccgacgtc tacggcgtcg acgcgctgcg ctactacctc ttcgccgcgg
    12661 tcggcttcgg ccaggacggg agcatctcgc ccgcggacct cgaagcgcgc tacaccagcg
    12721 acctggcgaa cgagtacggc aacctcgcgt cgcggaccgt gggaatgatc ggccgcttcc
    12781 gcgacggcgt cgtgcccgac gccacggccc cgccggagct ggccggcgcg ttcgagaacc
    12841 acgccgagcg cgtgagcgcg cacctcgacc gcgtcgagct cacacacgcg ctgtccgcga
    12901 tctgggagct ggtccggcgt gtcaaccagt acgtgcagga gaccgagccc tggaagctcg
    12961 cgaaggaccc cgacgcggcc gaccgcctcg acgccgcgct ctacggcatc gccgaggggc
    13021 tgcgcgtggc gtcggtgctg gtgtacccgt tcatgcccga gtcggccgag cgcgtcctcg
    13081 ccgccctcgg gcagccggag ctgaagctcg atcatgctcg catcggggcg gtccccggcg
    13141 gcgcccgggt cagcgagctc ccgcccctct tcccgcgcat agagaagtga tcgacagcca
    13201 ctgccacctc gacgcctgcg acccgcccga cgcagagctc gtccagcgcg cgcgtacgtc
    13261 gggcgtgcgg cggctcggca cggtgggcat gaaccccgct tcggtcgag
//
LOCUS       DASVPL010000249         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_185829, whole genome shotgun sequence.
ACCESSION   DASVPL010000249 DASVPL010000000
VERSION     DASVPL010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185829"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3213)
                     /locus_tag="VF517_13465"
     CDS             complement(<1..>3213)
                     /locus_tag="VF517_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8053993.1"
                     /translation="RGAALDWAAAAPPAGPALELPTYPFERRRHWLPEGGGDDGRAEP
                     PAEEPEAVADPTGPEGELLVRLLDLPPPDRRPELVAHLRTEVAEVIGLDSPDVLEPHQ
                     GFFEVGMDSLMAMQLQTRLAEALDRELPATLALDHPTVDALADHLLAEVLAQGSHATA
                     RRNGARRTRDDPIAIVGMSCRFPGAPDLDAFWRLLDDGVDAVTAVPPTRWDADAFYDP
                     DPDAPMRMNTRSGGFLDVPVDEFDAEMFGIAPREAAGMDPQQRLLLEVAWEALEDAGL
                     PVNELSGTETGVFVGINTSDYMQLLSSSGGADIDPYVATGNTFSVAAGRVSYLLGLQG
                     PSLALDTACSSSLVAAHLACQSLHGGECEAAVVGGVNLMLAPATSIGMAKLRALSPDG
                     RCKTFDASADGYGRGEGCGVVVLKRLPDAVAAGDRIAGVIRGSAVNQDGRSAGLTVPN
                     GPAQEALVRDALRRARVDPAQVGYVEAHGTGTPLGDPIELQALGAALGEGRDPERPLQ
                     VGSVKTNFGHLEAAAGVAGLIKVTLAMRHGRIPRHLHLSDPNPYIPWDELPIAVPTEP
                     VDWPRGDEPRVAGISAFGFSGTNAHLVVAEPPPEAAPVAAPEPAPVAAPPETAPAAAP
                     AERPDLLVLSARSPESLSDLAGRFATSLRERGDEFRDVCRSAALRRTHHAHRLAVAAA
                     SGHEAAERLAAFAGAGAAPGVVAGQPVADGRRLVFAYCGQGAQWVGMGRALAAAEPAV
                     QATLARCDELIRDRAGWSPIEELDAPEARSRLGDTEIAQPVLFSLQLALTELWRSWSI
                     RPDSVVGHSVGEVAAACVAGALDLEQAIDVVLARGRVMQPLKGRGAMAAIGLPPAEVE
                     ELLRAGVSVAAINSPSTTVVSGDTAAVEAVVAAAGERQAFARMLPGGYAFHSQQTAPC
                     REPLVEALASLEPREPAIPVISTVTGRRAGRDDLDAEHWGRNVTDRVEFAVALDAACD
                     GEHNLVVEIGPHPVLATAVTQCLAAAGKGGAAIASMRRGDGSRATALEALGALHVAGH
                     PVDHRRLREAGRFVSIPTYAWQRRRHWLRAARVTEPGRRVD"
BASE COUNT          386 a         1176 c         1270 g          381 t
ORIGIN      
        1 atcgacccgg cgtccgggct cggtcacccg cgcggcgcgc agccagtgcc gccgccgctg
       61 ccaggcgtag gtcgggatgc tcacgaagcg cccggcctcg cgaagccggc ggtggtcgac
      121 cgggtggccg gccacgtgca gcgcgccgag cgcctcgagc gccgtcgcgc gcgacccgtc
      181 gccacggcgc atcgaggcga tcgcggcccc gcccttcccc gcggccgcca ggcactgggt
      241 gacggccgtg gcgagcacgg gatgcgggcc gatctcgacg acgaggttgt gctcgccgtc
      301 gcaggcagcg tccagagcca ccgcgaactc gacccggtcg gtcacgttgc gtccccagtg
      361 ctcggcgtcg agatcgtccc tccccgcacg gcggccggtg acggtggaga tcacggggat
      421 ggccggctcg cgtggctcga gtgaggcgag cgcctcgacg agcggctcgc ggcagggcgc
      481 cgtctgctgg ctgtggaacg cgtagccgcc ggggagcatc cgggcgaacg cctgccgctc
      541 gcccgctgcc gcgaccacgg cctcgacggc ggcggtgtcg ccggagacca ccgtcgtcga
      601 cgggctgttg atcgccgcca ccgacacgcc cgcgcgcagc agctcctcga cctcggcggg
      661 aggcaggccg atcgcggcca tcgcgccgcg gcccttcagc ggctgcatga cccggccgcg
      721 ggcgagcacc acgtcgatcg cctgctcgag gtcgagcgcc ccggccacgc aggcggccgc
      781 cacctcgccc acgctgtgac cgacgacgga gtcaggccgg atcgaccacg atcgccacag
      841 ctcggtgagc gcgagctgga gcgagaacag gaccggctgc gcgatctcgg tgtcgcccag
      901 ccgcgagcgc gcctcgggcg cgtcgagctc ctcgatcggc gaccagccgg cgcggtcgcg
      961 gatcagctcg tcgcagcgag cgagggtggc ctggacggcc ggctcggcgg cggcgagcgc
     1021 gcgccccatg ccgacccact gggcgccctg gccgcagtac gcgaacacga gccggcgccc
     1081 gtcggccacg ggttgcccgg cgaccactcc gggcgccgcg cccgccccgg cgaacgcggc
     1141 gaggcgctcg gcggcctcgt gcccggacgc ggccgccacc gcgagcctgt gcgcgtgatg
     1201 agttcggcgc agggcggcgc tgcggcagac gtcgcggaac tcgtcgcccc tctcgcgcag
     1261 ggaggtcgcg aaccggccgg ccaggtcgct cagcgactcc ggcgagcggg ccgagaggac
     1321 cagcaggtcg gggcgctcgg ccggcgcggc cgcgggggcg gtctccggcg gcgcggccac
     1381 gggtgcgggc tccggcgcgg ccacgggggc ggcctccggc ggcggctcgg cgaccaccag
     1441 gtgcgcgttg gtgccgctga acccgaaggc gctgatgccg gccacgcgcg gctcgtcgcc
     1501 gcgcggccag tcgacgggct cggtcggaac ggcgatcggc agctcgtccc acgggatgta
     1561 cgggttcgga tcgctgaggt ggagatgtcg cgggatgcgg ccatggcgca tggcaagtgt
     1621 gaccttgatg agcccggcca cgccggcggc ggcctcgagg tgcccgaagt tcgtcttcac
     1681 ggagccgacc tggagcgggc gctcggggtc gcggccctcg ccgagggcgg cgccgagcgc
     1741 ctggagctcg atcgggtcgc cgagcggcgt tccggtcccg tgcgcctcga cgtagccgac
     1801 ctgagcggga tcgacgcggg cgcgccggag cgcgtcgcgc accagcgcct cctgcgcggg
     1861 tccattcggg acggtgagcc cggcgctgcg gccgtcctgg ttcacggccg acccgcggat
     1921 gacgcccgcg atccggtcgc ccgccgccac cgcgtcgggc aaccgcttca gcaccacgac
     1981 cccgcagccc tcgccgcggc cgtagccgtc ggcggacgcg tcgaaggtct tgcaccggcc
     2041 gtccggcgac agggcacgca gcttggccat cccgatgctg gtggcgggcg ccagcatcag
     2101 gttcacgccg ccgaccacgg ccgcctcgca ctcgccgccg tggaggctct ggcaggcgag
     2161 gtgcgcggcc acgagcgacg aggagcaggc ggtgtcgagc gccaggctcg ggccctggag
     2221 cccgagcagg taggagaccc ggccggccgc cacgctgaac gtgttgccgg tggcgacgta
     2281 cgggtcgatg tcggcgccgc cgctagacga cagcagctgc atgtagtcgg aggtgttgat
     2341 cccgacgaag acgccggtct ccgtgccgct cagctcgttc accggcagac cggcgtcctc
     2401 gagcgcctcc catgcgacct cgagcaggag ccgctgctgc gggtccatgc ccgcggcctc
     2461 gcgtggggcg atgccgaaca tctcggcgtc gaactcgtcc accggcacgt cgaggaagcc
     2521 gccgctgcgg gtgttcatcc gcatcggggc gtcggggtcg gggtcgtaga aggcgtcggc
     2581 gtcccagcgg gtgggcggga cggcggtcac ggcgtcgacg ccgtcgtcga gcagccgcca
     2641 gaacgcgtcg aggtccggtg cgccggggaa gcggcagctc atgccgacga tcgcgatcgg
     2701 gtcgtcgcgc gtgcggcggg cgccgttgcg cctggccgtg gcgtggctgc cctgggcgag
     2761 aacctcggcc agcaggtggt cggccagggc gtcgacggtg gggtggtcga gcgcgagcgt
     2821 cgccggcagc tcgcggtcga gcgcctccgc gagccgcgtc tggagctgca tcgccatcag
     2881 cgagtccatg ccgacctcga agaacccctg atgcggctcg agcacgtcgg gggagtcgag
     2941 gccgatcacc tcggccacct cggtgcgcag gtgggcgacc agctccgggc ggcgatcggg
     3001 gggcgggagg tcgagtaggc gtacgagcag ctcgccttcg gggccggtgg ggtcggcgac
     3061 tgcctccggc tcctcggcgg gcggctcggc gcgcccgtcg tcgccgccgc cctccggcag
     3121 ccagtggcgg cggcgctcga acgggtaggt cggaagctcg agggcgggcc cggccggcgg
     3181 ggcggcggcg gcccagtcga gcgccgcgcc gcg
//
LOCUS       DASVPL010000250         6883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_304301, whole genome shotgun sequence.
ACCESSION   DASVPL010000250 DASVPL010000000
VERSION     DASVPL010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304301"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VF517_13470"
     CDS             complement(<1..964)
                     /locus_tag="VF517_13470"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX8053994.1"
                     /translation="MSRPPLPEGPYLVVGLARSGTAAMRMLAPHGEVIGIDSGEPTEA
                     RELANVHLRSDGLDLLDSARTVVKSPGVPREAPVVAEALRRGIEVTGELELAWRLLPN
                     DFVAVTGTNGKTTTTELLGAIHRAAGVPVGVAGNVGTPLASLVGALPAAAVVVCEASS
                     FQLEDSSAFAPEAAVLLNLEPDHLDRHGNLDAYRAAKLRAFANQRPEHVAVVPPGFEV
                     PGEARRVEIDAGLLDGMELRLRGEHNRRNAAAAVAVALARGIDRESVANALRTFAGVP
                     HRLEEVAVRDGVTYVNDSKATNVASAAVGIRAFEGGVHAILGGSL"
     gene            complement(961..1938)
                     /gene="mraY"
                     /locus_tag="VF517_13475"
     CDS             complement(961..1938)
                     /gene="mraY"
                     /locus_tag="VF517_13475"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX8053995.1"
                     /translation="MLGEILIAGIASLLICVFLGPKFIDFLRAREFGQHIREEGPEGH
                     KHKAGTPTMGGLVMFLAVAVPFLILSDYDEASMAVFGTAMACAFLGFLDDWTKISHKR
                     SLGLSARWKLAIQALIAIALWYVATRGVGLDDELVTRLFDAEIDLGYFYPVLIFLVLA
                     GASNGVNLTDGLDGLAAGCCAIVLLAYTAMTLSTGDADIQRLSLLCACLVGAAVGFLW
                     FNSFPAAIFMGDTGSLGLGGAIAAMAVMTQTEVLLLIIGGIFVIEALSVAIQVFSFQR
                     FRKRVFLMAPVHHHFELMAWSETKIILRFWIVAAICSAIGFTLYQQSIS"
     gene            complement(1938..3362)
                     /locus_tag="VF517_13480"
     CDS             complement(1938..3362)
                     /locus_tag="VF517_13480"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX8053996.1"
                     /translation="MTLRDLIGEGAPAAEISGLAYSSGSVEPGYLFFCVRGFKADGHD
                     YAPDAVERGAAALVCERPLGLGVPEHVVPDARAAMGPLAARFYGDPTETLRVAGVTGT
                     NGKTTTTYLLRAILEAAGERTGLLGTVTSIVGGRAEPVERTTPEAIDLQRNFRRMLDA
                     GDTACAMEVSSHALDLRRTDGIHFATRVFTNLTQDHLDFHPSMEAYFQAKRRLFEGGA
                     PSIVNVDDEYGRRLAAELPDATTYAIECDAGYRALNVEFDPGGSRFTCATPDGEVRVT
                     TRLPGLFNVQNALAAAAAARSLGVPLDTAVEALARADRVPGRFEPVDEGQRFTALVDY
                     AHTPDSLDNVLRAARQITRGRLHVVFGAGGDRDRSKRPQMGEVASRLADRVIVTSDNP
                     RSEDPDSIVDQILAGTGPDAEREPDRRRAIELAMTSAAPGDVVVVAGKGHEQGQEFEG
                     GRKEPFDDVTVVREALRATQGAAH"
     gene            complement(3396..5033)
                     /locus_tag="VF517_13485"
     CDS             complement(3396..5033)
                     /locus_tag="VF517_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8053997.1"
                     /translation="MVLLERRIGLLFGLFLLLLVFAGLRATWLVGVRGDSLRGRAAQQ
                     QVQEIVVPARRGAITDRNGRELAVTEDAATISANPKQVPNPATAAHRLSAALRMPLDG
                     LLEKLSDRSVGFVYLARKVDAAKGDAVRKLSLPGISVTPEPRRRYPHGALASQLVGAV
                     GTDGYGLAGIEQSREKQLHGNDGKRRIVSDALGDPVSIVDLEHERPGQDVRLTIDSAI
                     QERVESVLSGVGRTYQPKGATALVLDPRNGEILSLANWPPVPAERFGEAPAYARQNRA
                     VGASYEPGSTFKPFTVAGALSEKLIQPSTLFQLGPTIQVADRTIKESHAGGGGTLTTA
                     DILAQSSNVGTVTIGLRLGGKRFDRWIRRFGFGGSTHVDIPGEATGIVPKPAEYSGSS
                     MGNLPIGQGLAVTPMQIAAGYQALANGGVMRPPHVIEGAPGPARRVIDARVAGQVSRM
                     LEGVLGAGGTAQEAKIDGYVLAGKTGTAEKAENGGYSKTKYVASFIGFAPARKPRLLV
                     AVMVDEPKGQIYGGQVAAPAFQKIASFALPYLRIPPG"
     gene            complement(5037..5921)
                     /locus_tag="VF517_13490"
     CDS             complement(5037..5921)
                     /locus_tag="VF517_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8053998.1"
                     /translation="MPRSAAARAETAAPAKPRTARAAAAATKSRSTARAAAAATKSRS
                     TGATKTRAAVAAPAKPRTARAAATAPAKPRTAPAPRRRSGPAQDARPRRGSAEATPRQ
                     PLTGADVRRAVASRGNVMIDRLLRGRLWVGCVGLLLAGIVSLNVGLLQLNDQIARTDS
                     KSASIGRENSDLRLQLAKLDSTERIQRLAEAKGYVMPAPGAVRYVRSRSRDAREAARR
                     IVAPEPITPAAAPAPAATGAPAAGATGATPTATPAPGTTAPTGTTAPTGTTAPTGTAT
                     PAATPAYTPPAATGTATP"
     gene            complement(5921..>6883)
                     /gene="rsmH"
                     /locus_tag="VF517_13495"
     CDS             complement(5921..>6883)
                     /gene="rsmH"
                     /locus_tag="VF517_13495"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX8053999.1"
                     /translation="AILLDMTRTHVPVLAGELIELLDPQPGETAVDCTFGDGGHARLV
                     AGRIGPNGTLVCIDRDPAAEERFDSFAREVSCGTRFLRMDYADGLELLVAEGVAADMA
                     YLDLGVSSMQIDTWERGFSYSYDAPLDMRMDSEQELDARTVVNEWDERRLAQLFRSYG
                     EERFARQIARAIVRRRAEAPLETTYELVEAIKSALPAPARFGGGHPAKRVFQAVRIAV
                     NDELASLDRALPPAWDVLAAGGRLGAISFHSLEDRRVKRFLAALARGCVCPPDLPICA
                     CGRTPEADVLTQRAVVPTSGEVAQNPRAASGRLRVARKLEGDEA"
BASE COUNT          910 a         2390 c         2663 g          920 t
ORIGIN      
        1 tgaggctgcc gcccaggatg gcgtgcaccc cgccctcgaa tgcgcggatc ccgacggccg
       61 ccgacgccac attcgtcgcc ttcgagtcgt tcacgtaggt cacgccgtcg cggacggcca
      121 cctcctccag ccggtgcggc accccggcga aggtgcgcag cgcgttggcc accgactcgc
      181 ggtcgatccc gcgcgccagc gccaccgcca ccgcggcggc cgcgttccgg cggttgtgct
      241 cgccgcgcag ccgcagctcc atgccgtcga gcaggcccgc gtcgatctcc acccgccgcg
      301 cctcccccgg cacctcgaac ccgggcggca ccacggccac gtgctcgggg cgctggttcg
      361 cgaacgcgcg cagcttggcc gcgcggtagg cgtcgaggtt gccgtggcga tcgaggtggt
      421 cgggctcgag gttgagcagc acggccgcct cgggcgcgaa cgcggacgag tcctcgagct
      481 ggaaactgga cgcctcgcag accaccaccg cggccgcggg gagcgcgccc acgagcgagg
      541 caagcggcgt gcccacgttg cccgccacgc ccacgggcac gcccgccgcc cggtgaatcg
      601 cgccgagcag ctcggtcgtg gtggtcttgc cgttcgtgcc ggtgacggcg acgaaatcgt
      661 tggggagaag gcgccaggcg agctccagct ccccggtcac ctcgatcccg cggcgcagcg
      721 cctccgcgac caccggcgcc tcgcgcggca cgcccgggct cttgaccacc gtgcgggcgc
      781 tgtcgagcag gtcgaggccg tcgctgcgca ggtggacgtt cgccagctcc cgcgcctcgg
      841 tcggctcccc cgagtcgatc ccgatcacct cgccgtgggg ggcgagcatc cgcatcgccg
      901 cggtgcccga gcgagcgagg cccaccacga ggtacggccc ctccgggagg ggtggccggc
      961 tcacgagatc gactgctggt acagcgtgaa gccgatcgcc gagcagatcg ccgcgacgat
     1021 ccagaagcgc aggatgatct tcgtctccga ccacgccatc agctcgaagt ggtggtgcac
     1081 cggggccatc aggaagacgc gcttgcggaa gcgctggaac gagaagacct ggatcgcgac
     1141 cgacagcgcc tcgatcacga agatgccgcc gatgatcagc agcagcacct cggtctgcgt
     1201 catcacggcc atcgcggcga tggcgccgcc gaggccgagc gagcccgtgt cgcccatgaa
     1261 gatcgcggcc gggaacgagt tgaaccacag gaacccgacc gcggcgccga ccaggcacgc
     1321 gcacagcagc gacagccgct ggatgtcggc gtcgccggtc gagagcgtca tggccgtgta
     1381 ggcgagcaga acgatcgcgc agcagccggc ggcgaggccg tcgaggccgt cggtgaggtt
     1441 gaccccgttg ctggcgccgg ccagcacgag gaagatcagc accggataga agtagccgag
     1501 gtcgatctcg gcgtcgaaca ggcgcgtgac gagctcgtcg tcgagtccca ccccgcgcgt
     1561 ggccacgtac cagagcgcga tcgcgatgag cgcctggatc gccagcttcc agcgcgccga
     1621 gaggccgagc gagcgcttgt gcgagatctt ggtccagtcg tccaggaagc cgaggaatgc
     1681 gcaggccatg gcggtgccga agaccgccat gctcgcctcg tcgtagtcgc tcaggatcag
     1741 gaacgggacc gcgaccgcga ggaacatgac gagcccgccc atcgtggggg tgccggcctt
     1801 gtgcttgtgg ccctccggcc cctcctcgcg gatgtgctgg ccgaactcgc gcgcacgcag
     1861 gaagtcgatg aacttcggcc cgaggaagac gcagatcagc agcgaggcga tgcccgcgat
     1921 gaggatctcg ccgagcatca gtgcgcggct ccctgcgtcg cgcgcagcgc ctcgcgcacg
     1981 accgtcacgt cgtcgaacgg ctccttgcgc ccgccctcga actcctgccc ctgctcgtgg
     2041 cccttgcccg cgaccacgac cacgtcaccc ggcgcggcgc tcgtcatcgc gagctcgatc
     2101 gcgcggcggc ggtcgggctc gcgctcggcg tccggaccgg tgccggcgag gatctggtcc
     2161 acgatcgagt cggggtcctc cgagcgcggg ttgtcggagg tcacgatcac gcgatcggcc
     2221 aggcggctcg cgacctcccc catctggggg cgcttgctgc ggtcgcggtc gccgcccgcg
     2281 ccgaacacca cgtgcagccg cccgcgcgtg atctgccgcg cggcgcgcag gacgttgtcg
     2341 agcgagtcgg gcgtgtgggc gtagtcgacc agggcggtga atctctgtcc ctcgtccacg
     2401 ggctcgaaac gcccgggcac gcgatcggcg cgcgccagcg cctcgaccgc ggtatcgagc
     2461 gggacgccca gcgagcgcgc cgcggcggcc gctgccagcg cgttctggac gttgaagagg
     2521 ccggggagcc gggtggtgac gcgcacctcg ccgtcgggcg tcgcgcacgt gaaccgcgag
     2581 ccgcccggat cgaactcgac gttgagcgcg cggtagccgg cgtcgcactc gatcgcgtac
     2641 gtggtcgcgt ccgggagctc ggcggccagg cggcggccgt actcgtcgtc gacgttgacg
     2701 atcgacggcg ccccgccctc gaacagccgc cgcttcgcct ggaagtaggc ctccatgctc
     2761 gggtggaagt cgagatggtc ctgggtgagg ttggtgaaca cgcgggtggc gaagtggatg
     2821 ccgtcggtgc ggcgcaggtc gagcgcgtgc gaggagacct ccatggcgca ggccgtgtcg
     2881 ccggcgtcga gcatgcgacg gaagttgcgc tggaggtcga tcgcctcggg agtggtccgc
     2941 tcgaccggct cggcgcggcc gccgacgatc gacgtgaccg tgccgagcag gccggtgcgc
     3001 tcgccggcgg cctcgaggat cgcgcgcagc aggtaggtcg tggtggtctt gccgttggtt
     3061 ccggtcacgc cggccacgcg cagggtctcc gtgggatcgc cgtagaagcg ggcggcgagc
     3121 ggtcccatgg ccgcccgggc gtcggggacc acgtgctcgg gcaccccgag gccgagcggg
     3181 cgctcgcaga cgagcgcggc ggccccgcgc tccacggcgt cgggcgcgta gtcgtggccg
     3241 tcggccttga agccgcggac gcagaagaac aggtagccgg gctcgacgct cccgctggag
     3301 taggcaaggc ccgagatctc ggctgcgggg gcgccctccc cgatcaggtc acgaagggtc
     3361 acgggtcggc ggcgagtgta cccccgggtc cggcgctaac ccggggggat tcgcaggtaa
     3421 gggagggcga acgaggcgat cttctggaag gcgggggcgg cgacctggcc gccgtagatc
     3481 tgacccttcg gctcgtcgac catcaccgcc acgagcaggc gcggcttgcg ggccggcgcg
     3541 aagccgatga acgaggccac gtacttcgtc ttggaatagc cgccgttctc ggccttctcg
     3601 gcggtgccgg tcttgcccgc cagcacgtag ccgtcgatct tcgcctcctg cgcggtgccg
     3661 ccggcgccga gcacgccctc gagcatgcgc gagacctggc cggccacgcg ggcgtcgatc
     3721 acgcggcggg cggggcccgg ggcgccctcg atcacgtgcg gcgggcgcat cacgccgccg
     3781 ttggcgagcg cctgatagcc cgcggcaatc tgcatcgggg taacggcgag gccctggccg
     3841 atcggcaggt tgcccatcga ggagccggag tactcggccg gcttagggac gatccccgtg
     3901 gcctcgcctg ggatgtccac gtgggtggat ccgccgaagc cgaacctgcg gatccagcgg
     3961 tcgaagcgct ttccaccaag gcgcagcccg atcgtcacgg tgccgacgtt cgacgactgg
     4021 gccaggatgt cggccgtcgt gagcgtgccg ccgccaccgg cgtgcgactc cttgatcgtg
     4081 cggtcggcga cctggatggt cgggccgagc tggaacagcg tgctgggctg gatcagcttc
     4141 tcgctcaggg ctcccgcgac ggtgaacggc ttgaaggtgg aaccgggctc gtagctggcg
     4201 ccgacggcgc ggttctggcg ggcgtaggcg ggggcctcgc cgaagcgctc cgcggggaca
     4261 ggcggccagt tcgcgagcga gaggatctcg ccgttacggg ggtcgagcac gagcgcggtg
     4321 gcgcccttgg gctggtaggt gcgcccgacg ccgctcagga ccgactcgac gcgctcctgg
     4381 atcgcggagt cgatcgtgag ccgcacgtcc tggcccgggc gctcgtgctc gaggtcgacg
     4441 atgctgaccg ggtcgccgag cgcgtcgctc acgatccggc gcttgccgtc gttgccgtgg
     4501 agctgcttct cgcgcgactg ctcgatgccg gccaggccgt atccgtcggt gccgacggcg
     4561 cccacgagct gcgaggcgag cgcgccgtgc gggtagcggc ggcggggctc tggggtcacg
     4621 ctgatgccgg gtaggctgag cttgcggacg gcgtcgccct tggcggcgtc gaccttgcgg
     4681 gccaggtaga cgaagcccac gctcctgtcg ctcagctttt cgaggaggcc gtcgagcggc
     4741 atgcgcaggg cggcgctcag gcggtgggcg gcggtggcgg ggttgggaac ctgcttgggg
     4801 ttggcggaga tcgtggcggc gtcctccgtc acggcgagct cgcgaccgtt gcggtcggtg
     4861 atcgcgccgc ggcgggcggg cacgacgatc tcctggacct gctgctgggc ggccctgccg
     4921 cggaggctgt cgcccctgac gcccacgagc caggtggcgc gcagtccggc gaatacgagg
     4981 agtaggagga acagcccgaa cagcagcccg atgcgccgct cgagcaggac catcgcctag
     5041 ggggtcgccg tcccggtggc cgcgggcggc gtgtaggcgg gggtcgccgc gggggtggcg
     5101 gttcccgtgg gggcggtggt gcccgtgggg gcggtggtgc ccgtgggggc ggtggttccc
     5161 ggggcggggg tggcggtggg cgtggcgccc gtggctccgg cggccggggc gccggtggca
     5221 gcgggggccg gggcggcggc cggcgtgatc ggctcgggcg cgacgatgcg gcgcgcggcc
     5281 tcgcgggcgt cgcggctgcg ggagcggacg tagcggaccg cgcccggcgc gggcatcacg
     5341 tagcccttgg cctcggcgag gcgctggatg cgctcggtcg agtcgagctt ggccagctgg
     5401 aggcgcaggt cggagttctc gcggccgatg gacgcggact tcgagtccgt gcgcgcgatc
     5461 tggtcgttga gctggagcag gccgacgttc agcgacacga tgccggcgag cagcaggccg
     5521 acgcacccga cccacagccg gccgcgcagc aggcggtcga tcatcacgtt cccgcgcgac
     5581 gcgacggcgc gccggacgtc ggcgccggtc agcggctggc gcggagtggc ctcggcggag
     5641 ccgcgccggg ggcgagcgtc ctgggcggga ccggagcgcc ggcgcggagc cggggccgtg
     5701 cgcggcttgg cgggcgcggt ggcggcggcg cgggcggtgc gcggcttcgc cggcgcggcg
     5761 acagctgcgc gggtcttggt cgccccggtc gagcgcgact tcgttgccgc agcggcggcg
     5821 cgcgcggtcg aacgcgactt cgttgccgca gcggcggcgc gcgcggtccg cggcttcgcc
     5881 ggcgcggccg tctcggcgcg ggcggcggcg gagcgcggca tcaggcctcg tccccctcga
     5941 gcttgcgcgc cacacgcagg cggcccgacg cggcgcgcgg gttctgcgcc acctcgccgg
     6001 acgtgggcac tacggcgcgc tgggtcagca cgtcggcctc gggcgtccgc ccgcaggcgc
     6061 agatcggcag gtcgggcggg cacacgcatc cacgcgcgag cgccgcgagg aaccgcttca
     6121 cgcgacggtc ctcgagggag tggaacgaga tcgcgccgag ccgtccaccg gccgcgagga
     6181 cgtcccacgc gggcgggagc gctcgatcga gcgaggccag ctcgtcgttc accgcgatcc
     6241 gcaccgcctg gaacacgcgc ttcgccggat gcccgccgcc gaagcgcgcg ggggccggca
     6301 gcgcggactt gatcgcctcg accagctcgt aggtggtctc caggggagcc tccgcccgcc
     6361 ggcggacgat cgcccgggcg atctgacgcg cgaagcgctc ctcgccatac gaccggaaga
     6421 gctgcgccag ccggcgctcg tcccactcgt tcacgaccgt gcgcgcgtcg agctcctgct
     6481 cggagtccat ccgcatgtcg aggggggcgt cgtaggagta agagaagccg cgctcccacg
     6541 tgtcgatctg catggaggag acgccgaggt ccaggtaggc catgtcggca gcgacgccct
     6601 cggccaccag cagctcgagc ccgtccgcgt agtccatccg caggaagcgg gtcccgcagg
     6661 agacctcgcg cgcgaaggag tcgaagcgct cctcggcggc cggatcgcgg tcgatgcaga
     6721 cgagcgtgcc gttcgggccg atccgcccgg ccacgagccg cgcgtgtccg ccgtcgccga
     6781 aggtgcagtc gacggcggtc tcgcccggct gcgggtcgag cagctcgatg agctccccgg
     6841 ccagcacagg gacgtgggtg cgtgtcatgt caagaaggat ggc
//
LOCUS       DASVPL010000251        62265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_69652, whole genome shotgun sequence.
ACCESSION   DASVPL010000251 DASVPL010000000
VERSION     DASVPL010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 62265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62265
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69652"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VF517_13500"
     CDS             complement(<1..891)
                     /locus_tag="VF517_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054000.1"
                     /translation="MSIRLAPLLVVGCVLVSASPAFAGVASAPRDPSTGAHRLEYRAE
                     PGESSRLSMSHATTGGVRVEDTGAPLAPGENCRSDGPNAVVCVAEQGYFDSAAFDLGD
                     GDDHLTLGLLEATVNAGPGDDQVISRQVKVDVDGGPGADLLQGLTNSRLSYADRTGAV
                     TVIQDGLPNDGEAGEGDNVGPGFTHLTGGAGDDELHLDERHRNGLWLRSSLEGGAGDD
                     RLFGTDSPDRIEGGAGDDELYGFAGDFDFLDGGPGADLIRGGAGFDGAAPYDGTRPVD
                     ITLDDRPGDGPAGENDDVGAD"
     gene            999..2129
                     /locus_tag="VF517_13505"
     CDS             999..2129
                     /locus_tag="VF517_13505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX8054001.1"
                     /translation="MAFDLEAALRRVIEIDGSDLHLKVPARPLIRTMGALEPIPDTDE
                     LTPEDTEGAVRELLNDPSKLEEFASEHEVDFSYSLPGCSRFRVNAFRQRGVVSMVARA
                     VPHEIKSIEQLGLPEVIGQIADEERGIILVTGTTGSGKSSTLAGMIDQINRLHARHIV
                     TIEDPIEFLHRDKRSIINQREVGADTASFKRALRRVLRQDPDVILVGEMRDEETVSTA
                     LSAAETGHLVLSTLHTVDAPETVNRIIDFFPPHMNQQVRAMLAGTLKAVVSQRLVKTA
                     DGRGRVACCEVLRMTGRVRDMIMNPEETGKLVDVIAEGAYYGMQTFDQALLAHVQGGR
                     ITMEEAMRSATSPHDFKLMVASDGQRSTSVEQLMGEPEPSVV"
     gene            2168..4480
                     /locus_tag="VF517_13510"
     CDS             2168..4480
                     /locus_tag="VF517_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HEX8054002.1"
                     /translation="MPVPDRIANPWGERTPLAPGDRWPERVDQHLTVPPDDVDAWVRA
                     ASVLHSNGDAYDLAVADGRIVGVRGRGDDRVNRGRLGPKDLFGWQATHSPDRLTRPLV
                     RMASGELEPVDWDAAMGLMVERSKELLAESGSGAFGFYTTGQLFLEDYYAQALVARAG
                     IGTNHLDGNTRLCTATAAEAMKQSFGCDGQPGSYDDINHADTIALYGHNVAATQPVLW
                     MRMLDRRAGPDPPTLIAVDPRTTPVAREADIHLAPKPGTNLALLNALVRELIANGWVD
                     RAYVDAHTVGYDDLESKTRECTPEWAERITGVAAADVRRAAEAIGGAERLVSTLLQGV
                     YQSHQATGTAVQVNNLHLLRGMLGRPGCGVLQMNGQPTAQNTRECGSNGDLPGFRNWQ
                     NEAHVEDLARVWNVDPLQIPHWAPPTHAMEIFRYAEQGSIRFLWITGTNPAVSLPELE
                     RIRAILRQERLFVVVQDLFLTETAELADVVLPAATLGEKTGTFTNAERTVHLAEKAVD
                     PPGEARTDLEIFLDYARRMGFTDRDGKPLIKFHDPESAFEAWKECCRGRPCDYTGITY
                     ARLRERSGIQWPCNEDAPDGTERLYTDGRFFASPEECESYGKDLLTGAALEETEYRAA
                     NPEGRAMLKACEYVKPVEAPDDEYPLALTTGRTIYHFHTRTKTGRAPELHAAAPEPWV
                     EMAPSDAEAAGVAEGDEVEVVTPRGRVRVPVRLTGMLPGVVFVPFHYGYWDAPGADHD
                     RAANELTITAWDPASKQPLLKVGKARVQRA"
     gene            4477..4962
                     /locus_tag="VF517_13515"
     CDS             4477..4962
                     /locus_tag="VF517_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054003.1"
                     /translation="MKLGLAIEQLAESEERLATELARAGERHEADHDVYHLSSTLAGI
                     SRAHLKALAPFAERYGVAVETNGAGPASGLLGAVRERASELTGRRPEAGLLLLRDLRE
                     LYLLASDASLGWTVLGQGAQAARDSELLDVVSECHPDTLRQIKWITTRIKQAAPQALT
                     T"
     gene            5045..5770
                     /locus_tag="VF517_13520"
     CDS             5045..5770
                     /locus_tag="VF517_13520"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEX8054004.1"
                     /translation="MQSTLGQFQETESTEPFSLQNSKLLKVELNQVTIQAKLGSMVAY
                     QGDVTFEHAGSGGMGRMLKKAVTGEGTKLMKMSGAGEVFLADQAQDIHLVQLENDYIT
                     CNGANVLAFDAGIDWDIKKVEGGVAAAMAGGLTNMTLQGTGWVALISDGPPVLLDVST
                     APTFADAQAAITWSSGVQTQVKVDANLKSLIGRGSGESIQMAFSGQGWVLVQPSEGMV
                     QAAAAGGGGGGAAGGLAGMLGGR"
     gene            5856..6221
                     /locus_tag="VF517_13525"
     CDS             5856..6221
                     /locus_tag="VF517_13525"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054005.1"
                     /translation="MKQGSDVCPVCATAELVCTKWTMLIVRDLAQGRSRFCEFERSLA
                     GISPRTLSLRLRELEAEGVVERRTYPEVPPRVEYALTEKGQALLPIIEVMRDFGREWL
                     DAHGPCDVPDEARAPAAVA"
     gene            6270..8252
                     /locus_tag="VF517_13530"
     CDS             6270..8252
                     /locus_tag="VF517_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054006.1"
                     /translation="MRNRELHDALRDFALESAALLTAAVGAGAELPYDVLEEPGTGSV
                     LYRYQPLTAEFIGARWDSISAQSSFERAARALGTGAEAYLRMRGLPGGADSEPALRAL
                     LERLYEDATGFEFPEERFERVYAEFERTLYEHTLRASVVAPVHGLVLERERVELGGGL
                     ILARGDRVAAPPEAVWPLGPGERDRATGPNTVVALERDVEADAGMPAGEARIRFRRLL
                     TALRLLKPGRVAFGAPAWGRADEGAWQPIPLSFGGRGRGEPWLLRASEGGELAELVEL
                     VSAARLPGRIAWALGRFEMGCERGSDAEALSDYLLALRAMLDADDETGRASLGLRLAA
                     LCAEESERRGVRARLETAFALERSAMGGAVLDPDDTAPGAVVHELEQHVRALLRDVVC
                     GYLGGDLRSVADDILLASADPIEIRARDVRAEPDEVPDLRDPAPDLLDEPPPRAEERE
                     EGFPEPLATGEHDPGLFARTGDEDWEEPDDAAEPDADGGPLLRRVRRGDGPGTAADPR
                     SAAWRIASQPGPRRGRERPEPAATPASVMADEELTIADRPMAAAVAEPPVAEPRRAEP
                     RSAEPPRAEPTVAEPRAEPRSAEPPVAEPRAEPRSAEPPDPLAAAAPEPLLPPEEPTS
                     RIAALDAPEIDVDHTISAGVTPSADWDDDDCWSAPV"
     gene            8315..8557
                     /locus_tag="VF517_13535"
     CDS             8315..8557
                     /locus_tag="VF517_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054007.1"
                     /translation="MTEGKAFRSPGLKVDGRIFAMLVGEELVVKLPAERCKALVTDGA
                     ARPFESGSRKMREWVVIELPPTRWPELAREALSFVR"
     gene            complement(8554..9675)
                     /locus_tag="VF517_13540"
     CDS             complement(8554..9675)
                     /locus_tag="VF517_13540"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX8054008.1"
                     /translation="MSVGELLDGKHVCICAGSGGVGKTTTAAAVAMGMAAQGLRVAVL
                     TIDPARRLANSLGLSELGNEATRIDPALFEEHGLEMRGELWAMMLDAKRTWDELIETR
                     APDDATRDAVLGNRIYQELSNALAGSTEYMAIEKLYELHESGDYDLLVLDTPPTRNAL
                     DFLDAPDRLTRFIDSRSLSFFLKPGLFGVKAFGRGTGVLFSLLKRLTGVDLLRDLSEF
                     FASFGDMAIGIRDRAKVVESLLEDADTTFLLITSPRRDAVDDVVYFRRRLRENGMPFG
                     AAIVNRVHEGADTDVSGADVEPDLGALLGEKLGRKVARNFDDYRELAARDADNIARLE
                     RELDGDPLVRVPLLDDDVHDLAGLAAVNDYLFAADAVTA"
     gene            complement(9672..10607)
                     /locus_tag="VF517_13545"
     CDS             complement(9672..10607)
                     /locus_tag="VF517_13545"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX8054009.1"
                     /translation="MSDLLDKRLLFVAGKGGVGRTTVAASLGLLAARRGKRTIVVEVA
                     KQERVSRSFRHAGAGYAETELAPNLYGISIDPDKSLEEFLVDQVGSKRLAGLLFNNRI
                     FEYFAAATPGMRELVTIAKVWELALLERRRRGDAPYDLVILDGPPTGQGLGMLKTPQT
                     FADVARVGPIRRKALMIDGFLRDPAQTGVVAVALPEEMPVNETLEFRTKLRDQMGYDV
                     GLVVMNAMLPERFGGEDAAAIEAVNGGAGDPTVAAALRTALSQHHRSRAQRAQLRRLR
                     KGRDGAPVLTLPFLFDTDLDIDDWEGLSGELERRL"
     gene            10760..11509
                     /locus_tag="VF517_13550"
     CDS             10760..11509
                     /locus_tag="VF517_13550"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8054010.1"
                     /translation="MIVFANQKGGVAKTTTTLNLACAFAEKGHRVLCVDMDPQGNLTM
                     SQGIDPDSLESSMYDVLVHHIPIRDVIRKREIDVACASIDLAGAEIAMSTQIGRERSL
                     EKAFAGIKEDYDYICIDTPPSLGLLTINALTAADKVIVPVQCEYLSMRGLIQLQNTLS
                     MIRDNLNPKVRIEGIVPTLVDSRTTHAKEAIELLEENFGDRVYASRITKTVRFAEAPV
                     KGMSVLKYDPDGKAAYAYRQLAREVLANGSR"
     gene            11520..13097
                     /gene="ftsZ"
                     /locus_tag="VF517_13555"
     CDS             11520..13097
                     /gene="ftsZ"
                     /locus_tag="VF517_13555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX8054011.1"
                     /translation="MREGPLAQLFRRTDEDAPEPGREQAPAEPQAAPPEERAAQSAHE
                     RLDGPIARAETHDEPRTPDQERAARREAERRSPVEGLEDLRPAPPSARQHHEPEPRRE
                     PVEPPSPTTPHREPGVRPPKQRLEEVFSNEVPDDMLARTPRRDDEYRGRYGRDVPEPT
                     AVTPEPVLQPVLRVVGVGGAGVNAVNRMIEAGVEGVDFIAVNTDVQSLQQSSAHITQH
                     IGSDVTRGLGSGSDPEIGRQAALDSYDDVKALLRGSDMVFIAAGSGGGTGTGAAPVVA
                     RCARELGALTVGIVTKPFTFEGSRRADQAERGIEELASEVDTLITIPNTRLLSVLDKR
                     VSMVDAFRMADDVLRQGVQGISDLITLPGLINLDFADVRTIMANAGPALLGIGMGVGE
                     QRAMEAATHAVASPLLETSVEGARSILLSITGGRDLSLWEVNEAAKAVAEAAHPDANI
                     IFGAMVDENVVDQVWVTVVATGYGERPARRASSGREGGRASWEPEGEPRVTRGAGRSQ
                     NGSALSDIDIPEFLPRS"
     gene            13173..14633
                     /locus_tag="VF517_13560"
     CDS             13173..14633
                     /locus_tag="VF517_13560"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX8054012.1"
                     /translation="MLRAGGNAVDAAVAGVLMSFVTESPLTGPGAGGFMLVHTAGGED
                     HLLDFFVAAPGRGLAEGTEPAALRPIELRFSEEAVQTFNVGASSVGVYGTTVGIAQAL
                     RRFGTVRLGDLTGFAAQVARDGHEVTPMEGSFIEILGPILTSTPEAAAAFAPDGRVPV
                     PGDTLRLPELGDLIERLGAEGPGFLYVGDVAEAVCEWVGERGGLLTLEDLRSYQVIER
                     EPARAEYRGRTMLTNPPPSSGGILIAYSLGLLERLGRPADVGTVVEVMDQANRARTDE
                     FLQGLHAEGYLERFLAKDALEKAASRLGSTTHISVLDDDGGCAAVTCSNGSSSGVFVP
                     GTGMHLNNMLGEEDLNPFGFHQHAPGRRIPSMMAPTVVLREGHPQIALGSAGSNRIRS
                     AILQTILRVIDDGMPAQEAVTHPRVHWEGRVVEAEPGVDEEALAALEAGGREVIRFAE
                     QSLYFGGVQAVARDPETGALSGGGDPRRGGAAVVVP"
     gene            14721..15137
                     /locus_tag="VF517_13565"
     CDS             14721..15137
                     /locus_tag="VF517_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054013.1"
                     /translation="MLPKCKPFGGAALAATTALTALACGNPGGDLIALEERGAHFTRL
                     VVTDDGRGSCNGGELKRLESSRLLEAREVERELQELATERTNFPDPTGGRQFRAKMRD
                     ATVTWMERSQQDPVLPKATALTLELRRELCPAPATP"
     gene            complement(15134..15751)
                     /locus_tag="VF517_13570"
     CDS             complement(15134..15751)
                     /locus_tag="VF517_13570"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX8054014.1"
                     /translation="MAHVEERFPLFPLGIVLLPTEIVPLHIFETRYKLMIGESVESGG
                     EFGIVWLGDDGLAEVGCTARVAQVLEQMDDGRMNILAQGGRPFRLLRKIDDMPYPAGD
                     VEMIEDLDEGDEELAAGAREAYADLVERVTDNRPGIEELEGLTAYGMAATIELAAAPK
                     QALLEERSEDRRLKTVGDLFVTALDRIDNAEEAQERAKSNGKVRF"
     gene            complement(15780..16868)
                     /locus_tag="VF517_13575"
     CDS             complement(15780..16868)
                     /locus_tag="VF517_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054015.1"
                     /translation="MTSSTISQPLNNAALPAQTGGFVIKQPVNFTGGDALIGFSASAW
                     SGQPGPISMSLWLDGQPTGAMLGTYASQGAMHLSLGHTWAWVHGLTPGQHVIMLETGP
                     ATVTDPNDVVCLTVIEPNDGLAIRLAEDAPCPTGVAQPLITARAETKGGGVWISGSSS
                     GWVTQAATTVRSALLYDGGDGAPMEVFANNANQRLTVVPTDNVEDTSRGNHEFQVFGG
                     GQTQTDPNDYAHLSVVEWVDYAQAPSYLAGITNAAAQAQQGSGTIASVPVTSKGGTLL
                     VKGAASCWTQTANAPLVMSVVIDSTPVGMLRIFANPTSVHMAAVTNDLVVPNIPAGSH
                     TLSLVGQPNTITDQNDRVSAFVMEFPKS"
     gene            16924..18264
                     /locus_tag="VF517_13580"
     CDS             16924..18264
                     /locus_tag="VF517_13580"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX8054016.1"
                     /translation="MTAIVWFRRDLRVRDHPALRRAIAEHDRIVPFFCLDPRLLHGRH
                     ASGPRTQFMLECLADLDESLDGRLVVRHGRPEEELVRIAREAGAGDVYFSADVSPFAR
                     QRGSRVNEAGLRLHACPGLTVVDDVGALQTGTGRPYTVFSPFHRRWSGEPRRAVLRAP
                     RKLAGPSLSGAIPSLTELGLRQEVEEPMPGGETAARRRLAAFSIEDYSREDLESSSRL
                     SPYLHFGCVSPLEVETREPGLGRQLAWRDFYHHVILRFPRNAVQEHQERYRGSLAWDH
                     DGELLSAWQEGRTGYPLVDAGMRQLRREGWMHNRARLVVGSFLTKHLGLDWRLGERWF
                     MRLLIDGDEANNNGNWQWIASVGVDPQPAYRRLYNPARHLERFDPDGTYVRRYVPELQ
                     RVPMTHLPEPWKMPPELQRQAGCTIGRDYPAPIVDHREARAAALERYRSAAAAA"
     gene            complement(18204..19166)
                     /locus_tag="VF517_13585"
     CDS             complement(18204..19166)
                     /locus_tag="VF517_13585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054017.1"
                     /translation="MPPVSHEEIREVNVRYHDVAAADYDSKWGISYGPDGRAQVVGKM
                     RRALGADRRFGRALEIGAGTGYFTLNLLRAGMIDSAVATDISPGMLRELEDSARRLGV
                     QVETACSEADALPFPDDSFDLVFGHAILHHLPDLDAAFREFRRVLRPGGRIAFCGEPS
                     RHGDRIAGVPKRTAMALAPAWRRVLGARERGLPESDGHDHALEHVVDVHAFTPGQLEE
                     LATGAGFEGVRVRGEELVAGLFGWMNRTLESTAEPDSIPLAWIRYAYFGYLGLKQVDA
                     RLLEGRLPPAIFYNLLVSAQAPAEPQAAAAADRYRSSAAARASR"
     gene            19210..19974
                     /locus_tag="VF517_13590"
     CDS             19210..19974
                     /locus_tag="VF517_13590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054018.1"
                     /translation="MSEPASYTQQDQPPGVPPLSLTGERTLPDVPEENYWYRRHLAVY
                     RWVAERCRGVDVVDMACGEGYGTAVLAERAARVTGVDANPEAYEHARTKYTRPGVRFV
                     RDLVEGYSEPCDAVVFLQTIEHLEDPKAVLDHFRSILRPGGAAYVSTPNLLTLAPPGA
                     EKSDNPWHVREFRAEEFRELCEASFERVELLGLFHARKLRAHELAISHGWDDLHPKLG
                     ITKRFYDWFTPAISEHDFALRPAPLDGALDFVAVLR"
     gene            19971..21446
                     /locus_tag="VF517_13595"
     CDS             19971..21446
                     /locus_tag="VF517_13595"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEX8054019.1"
                     /translation="MSALRADPDRGQLAIVLHTHMPYVEGFGTWPFGEEWLWEALTGV
                     YLPLLDVLRDAPVTLSLTPVLCDQLEALEGEAGERYLSFMREMRAPIHAEDARGLDSV
                     GAHDAANELRRAAADYREGERRFTELRGRLLHELRELDRHSPVELWTSAATHSVLPLL
                     ATDAGLGLQVSTGIRSHERRFDEWSGGFWLPECAYRPGLERDLAEHGVRAFCVDQTES
                     LGLGSLDQLEPVRTEPGPVAVPIDWELTKLVWDSAGYPSAALYRDYHGRTTHDLRPWD
                     NGGEPYRHWQALVLARRHAREFVAHAAGRLDAYRAERGRPGLLCCALDTELLGHWWYE
                     GLRWLAFVLDEARLQGVPLGTLPDALDRVEAVERPLAAASWGKPKDLTTWDSPRVADI
                     AFAARRAELDTVAAAAAATARDRGPALQRAVRELLALQASDWAFQVTHDLAADYPRKR
                     VESHARELDAALAALKDSGAPPDPAVRNLAPDVDLAPLFAT"
     gene            21463..22653
                     /locus_tag="VF517_13600"
     CDS             21463..22653
                     /locus_tag="VF517_13600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8054020.1"
                     /translation="MTRVLILSWEYPPLIEGGLARHVRKLAENLVGLGVEVHVLTRGH
                     EESPAEEELRGVMVHRVREPERPRELAEFVTWVEHMNADMLAAGVELGDRYDFDVVHG
                     HDWLVAVAGDHLARRFRCPLAVTIHATEYGRHQGWVDKHPQSHIHGVERWMANRADRV
                     ITCSAYMREHVADIYGLEEDRIAVIPNGIDPSDLVTVEDLDALRARFAAPDERLVLLV
                     GRLVYEKGFQLALEALPGLVERVGNVRFLVAGSGTHEDELREQATELGLDEHGTFLGW
                     IGDDVLHSLYRIADLTVVPSIYEPFGLVALEAMASGCPCLVADTGGLREVVPNDEVGL
                     RFRSRDPESLGRMAERLLTDDELRDRLIAEASEHVLTFDWADVAEQALTVYAELARDR
                     SPAT"
     gene            22678..24873
                     /locus_tag="VF517_13605"
     CDS             22678..24873
                     /locus_tag="VF517_13605"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8054021.1"
                     /translation="MRALASFVTGRRTKRVVPAIWLLLLLVFTPLGAQLTDAVDDRFE
                     SFLPDDAQSTEVAELQRERFRSGQTADGLVVYRREGGLTAADRRKILADAQAAQRDPD
                     LHLKRPPVAAGQPGAPADLFAPDGDAALASYTFENDFETLGDEGEALREITGDGGGGL
                     EVYVTGQAGFGADFKEVFGELDTKLLLATVLLVLVLLGAIYRSPLIAISPLIVVAFAY
                     MVAQGLIYLYTQTGAVVNNNATQILVVLMFGVGTDYCLLLVSRYREELRRVEDKHEAM
                     ARAMRRTGPVIVASGMTVSLTMLTLLVADTGSIKSLGPVAAIGVMCVLAASLTLLPAL
                     LTMFGRPGFWPRRASVEFSPDDEVHERSGVWRRVGGRVLRRPGLALIVTLAIFGLGSL
                     GLFAYAEDYSTENGFKQKTESVEGFKALSESFPSGATDPSTVMVVREHGSVNDRELAA
                     VAARVQSDGAVAAAAPVPERSTDGRIGQVSVVFREDPYTPAAFDAVPRIRDEVADAVP
                     GARTIVGGGAAVQYDFDASTERDLKWIVPLGLIVIGLILALLLRAAVAPLVLIASVMA
                     SFFGTLGISLLFFRHVVGDPGVDASLPVFAFIFLVGLGIDYTIFLMGRVREEARIHGT
                     REGTLRALAATGPVITSAGMILAGTFSVLITMPITFMFNIGFMVALGILLDTFVVRTV
                     MVPAAVEVLGDRIWWPSDPSGGGKALSEDRPGPPEPATPSAAPSASPAS"
     gene            complement(24833..25465)
                     /locus_tag="VF517_13610"
     CDS             complement(24833..25465)
                     /locus_tag="VF517_13610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8054022.1"
                     /translation="MLRRPPPPLHGGPLTLMRFMRRHRMLNAKYARLAVRLLRRRLTN
                     RRLHSDGLAFIGPGVVLQIGRRGRIELGRWSWLGHGTKIRCHEGVVSIGAKTVLGQEC
                     TISAYQHVSIGRECVIADRVMLIDFDHGVVEVERPIRLQGIYKRDVRVGNNVWIGYGA
                     CILRGVTVGDNAIIGTNSVVTRDVPANAIVGGVPARLIRMRETPRELRWE"
     gene            25492..26448
                     /gene="meaB"
                     /locus_tag="VF517_13615"
     CDS             25492..26448
                     /gene="meaB"
                     /locus_tag="VF517_13615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX8054023.1"
                     /translation="MTPLGTTAAELTGRLLAGDKRALARAISLVENDDPEGWALVREV
                     YPKTGRATMVGFTGPPGVGKSTLIGAIVKNARERERQVAVLSIDPSSPFTHGALLGDR
                     IRLADHFLDPGVFIRSMASRGSLGGLSEATLQAALLMDASGKDDVLLETVGVGQAEVD
                     IIDHADTVVLVLMPGSGDSIQALKAGVMEIPDVIVVNKSDHPLTDTMIREIRGVLSLG
                     PSGSWKVPILRTEAAKGEGVSELVEKIQEHREHIEAEGTLQERRRRNLMNEVLELATV
                     RMRRKLDATARENDEVHALLERVVSRELDPASAAAQLLERLT"
     gene            complement(26477..27865)
                     /locus_tag="VF517_13620"
     CDS             complement(26477..27865)
                     /locus_tag="VF517_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054024.1"
                     /translation="MRRRALLLWIVAFGLYASTIGIHAFGASEYGGDEPHHLLTAQSI
                     VDDRTPDLLDEYRSRAYSEYYPYEMRPRGVLTAGNLNEPHGVGFPLLIAPAWAAGEET
                     AVELFLAAIAALAVVLAYLLALRAVPDPWALGATLAVAASPPMLAYSTAVQPPLAAGV
                     ALAGATLLALRLDEHPSRRSSFACFLLLATLPWLGMSFVPAGLVVGAIAFRTLRNAQR
                     PWVTLVGLEVVAVSIAIYLSVNDNLYGGPTPQSAGLGDEATGTNASFPGGYLDRAYRL
                     AALLIDREAGLLRWAPVLALALAGAWLLLRERQGRLARAIPALRRGERAVTICALAVG
                     AQFLTAAFLAPTMFGFEFPGRHLIAALPLGVPLVALGLRRLPRVGASLIAVTLVGSGW
                     LYADLRWGGGTWIDDRPDAPFGPLTELLPLFGEGSVAPFVVAAVLGAGALAALALALS
                     GSRWRALAGGGP"
     gene            complement(27927..29060)
                     /locus_tag="VF517_13625"
     CDS             complement(27927..29060)
                     /locus_tag="VF517_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8054025.1"
                     /translation="MNFDLTDEQRNIRELVRDFAQNEVRPVAEELDREKRFPYEIVAQ
                     LGELGLMGMPFPQEYGGGGTDNLSYAIAVEELTRVDSSVAITMAAHTSLGTWPVYAFG
                     SEEQKRELLPDLCAGRKLGAFGLTEPEAGSDAGNVKTRAALEDGEWVIDGAKQFITNA
                     GTDISGHVAITAKTGENEISNILVPKGTPGYEQGTPYRKMGWNASDTRPLSFDGARVP
                     EENLLGPRGQGFKQFLQTLDGGRIGVAAMGVGLAQGALDEALAYSKERRAFGQPISKF
                     QAIQGKLADLATEIEAARLITYEAAWLKDQGRSFTLVAAKAKLKTGRLAVRATEEAVQ
                     IHGGYGYIEEYPVCRHYRDAKILTIGEGTDEVQQMVIARALGA"
     gene            29138..30037
                     /locus_tag="VF517_13630"
     CDS             29138..30037
                     /locus_tag="VF517_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054026.1"
                     /translation="MRSERGQASVEWVGAVLLVALVLGGATAAAGDVDGRSYGGWLAH
                     SIVCAVSRGCAEQAGELAAAYGDSDAELVRRYAPNLVYEPGTYTLPVDFRQCRSHRCA
                     DAPDDPDLDAHLTTRGGYPATAFTHIVHRDGETFIQYWLYYPDSNSTFAGSSRAWNAV
                     RRVEPWMGDYPGYHRDDWEGVQVRIGPDGRARTRASAHHGYQWCKQQRCKNSWGPWTG
                     WSRVSRGSHAGHVPTRSDPLSTRIRPAHPGADTHERTTTSGGLRLIPIETLDTGAYKP
                     LDPGITPPWRKRVYTDPLSDSTS"
     gene            complement(30044..30796)
                     /locus_tag="VF517_13635"
     CDS             complement(30044..30796)
                     /locus_tag="VF517_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8054027.1"
                     /translation="MAGALDGRKVAITGASSGIGAATAELLAAQGAAVAVGARREERL
                     GELVERISGAGGTAHAVPVDVGDEASARSFVEGAAEKLGGLDGLVNNAGVMLLGPIDG
                     ADTEHWRRMVDVNLLGLLYCTHAALPILREGGGGDIVNVSSVAGRVASAGSGVYNLTK
                     WGVGAFSEALRQETGWAKIRVTIVEPGFVDTELQGHNDIPVVVETIGKMREQIGKVLE
                     PGDIANAIAYAISQPEHVSVNEILVRPTGQPR"
     gene            complement(30796..32169)
                     /locus_tag="VF517_13640"
     CDS             complement(30796..32169)
                     /locus_tag="VF517_13640"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX8054028.1"
                     /translation="MPVFEGETLDDPALQALVDSGEAKAGLRKLAVTHEEGRRVVLVG
                     LGKREELEHERARVAAAVAAGRARELGARSLSWAAPTGGEVAGALVEGTLLALYRFDQ
                     FKSTKEDGDDAAIGSLEIASAEGVDPQAIESGRVRGVAQNAARDLQNYPANVATPTWL
                     AERASEIAAAHDSLTIEVWDRAQIVEAGMGAFASVAQGTYEEPRMIVMRYEGGGSGPL
                     LGYVGKAVTFDTGGISIKPAGRMHEMKFDMSGGAAVIEAMGAIAELGVPARILAVVPS
                     TENMPSGRSVKPGDIVTAMNGKTIEVNNTDAEGRLLLADALAYAVDQGAERVINLATL
                     TGAIIVALGSTYAGLFSNDDDWCGEVEAACNATGELGWRLPLHPEYLELTKGKYGDLT
                     NAAEERKAMSIYAAEFLRQFVGETPWVHVDIAGTAWGLGRSYVGKGASGFGVRMLVEL
                     AEQAGRS"
     gene            32300..33484
                     /locus_tag="VF517_13645"
     CDS             32300..33484
                     /locus_tag="VF517_13645"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8054029.1"
                     /translation="MPKTVILGTARTPFGKLGGALSSLDATDLGGHAIAAALERAEVA
                     PEQVEHVSFGQVLQAGQGQIPSRQAQIKGGIPKEVSSETVNKVCASSIRSVTIVDSAV
                     RAGDLDVAVAGGMESMSQAPYLLKQARFGYRMGDAKALDAMINDGLTNPFTKKHMAQE
                     ASEVGDELEMTRPDLDKFALRSHELAIQATDEGRIPEEIAPVTVKAKKKETVVEVDEA
                     PRRDTSLETLAKLPPIFVEDGTHTAGNAPGVNDGAGALVLASEEWAKQNGRQPLATIV
                     THAQVADDFPYLARTPANATKKALEQAGLTVDDIDLFEINEAFASVAINSMRMLEVDE
                     SKVNVNGGAIALGHPIGASGARILGALVHELRRRGGGRGVAAICSGGGQGDAVIIEVN
                     GG"
     gene            33607..34083
                     /locus_tag="VF517_13650"
     CDS             33607..34083
                     /locus_tag="VF517_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054030.1"
                     /translation="MRGRHCAVVCALVAAAVSSTAGPASAQIFPGDCFVSDGGRILTA
                     TGSPATFGGSASSTSGAQLGHQVYIDDGPLTGFRFRSIAMTAMLCNLDAHRAEMYGSG
                     EVTTQLGLSQPVEYRIAVLGVRSTSQVPDTYRITLSNGYDSGEQPVIDGNIAVRGT"
     gene            34108..34908
                     /locus_tag="VF517_13655"
     CDS             34108..34908
                     /locus_tag="VF517_13655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX8054031.1"
                     /translation="MQRGAAFFDLDKTLIEGSSAVHFARSSYRAGVMTRGQLARDLWA
                     NVKFRLEGSTDDMADMLRSRVLDAIAGRRVVDLARLAPGALAGILPRVYPEMLKEAWA
                     HQDAGRPVYIVTAASQEMAEMMAHVLIFDGGVGMRSEIADGVYTGRPDGPFTYREGKA
                     VAIREMAEREGIDLAESFAYSDSESDLPMLRLVGNPVAVNPDAHLSRVAREEGWRIIT
                     FDRLHRRVRIATTAAALAGVGLAGGWVAGRARTLSAGWGRRRTRLPLR"
     gene            34872..36626
                     /locus_tag="VF517_13660"
     CDS             34872..36626
                     /locus_tag="VF517_13660"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054032.1"
                     /translation="MGKASYTLAVALIARSSAPRALAALAAVIAFAAAPAGAQTPAPH
                     PLTGKGQNFEIVANVPMDGGSGEVAASDLELHGDYAFVGSYGEGMVVVDISNPFAPKR
                     VGKFDCPGGQNDIQLSPDGKTVAMAIDTTANECHPNDEGTSIIDVSDPAQPKEIAWID
                     KKELPRGSHNNTLDWPYLYVDQYSTAYSQLEIFDLSTPSVPRKVGELSYGGEDSVHDL
                     SVDHRPDGRSFAYAASIGFTDVIDVTDPRSPILRERVQDPQVTISHQAEANFDRTLLL
                     VTDEFNGGASYSGACGGSPITRVPFPTGAPAVGDPQNIGALHIYRLDPSGNIAGSGGL
                     DKAGTFNIPYQANDEPSAGCTIHVFWQAPDENRMVTAWYGRGTRAIDYSNPAAPKELG
                     YFIPTGSDTWAAKPHRGFIYTGDILRGMDVLRYKGEGGARWPATSGPAELQRARQQGA
                     SPPVPTGAPAPAQPAPQQGEQPQPTQGPRLRVGRRVFTKHVRVPDVPRKRKAELVLSF
                     YKNKGLVTRLRLRTRDRRIAKLRTQAAGLAGHYRYTIRLGDRGRFLARGIVRVRRSDT
                     TRVELPAGQALICRVSAK"
     gene            36636..38285
                     /locus_tag="VF517_13665"
     CDS             36636..38285
                     /locus_tag="VF517_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8054033.1"
                     /translation="MATAPRAMTDAERWYRERYSASPERDALFTTLSGEEVEPLYTAD
                     HLGDPDGIGLPGEFPYTRGVYPSMYRGRLWTMRQFAGFGTAEETNERFRYLLDRGQTG
                     LSTAFDMPSLMGHDSDHARSEGEVGREGVAIDTLDDMETLFSGIPMAEVSTSMTINAP
                     AAVMLAFYVVAAEKQGTPPEKLAGTIQTDILKEYIAQKEWCFPIEPAMRLVGDMIEFC
                     SRRMPRWHPVSISGYHIREAGSTAQQELAFTLKDGFTYVEQAIERGLHPDEFAPRLSF
                     FFNAHIDFFEEIAKYRAARRIWARELRDTYGAQDPKSLLMRFHSQTAGVSLTAQQPLV
                     NIVRTATEALSAVLGGTQSLHTNSYDEALALPTEDAVSIALRTQQVIAHETGVTNTID
                     PLGGSYFVEAMTDRMERAAYDYFAKIDELGGMVEAIKQSYPQREIADASFRYQQEVED
                     GQRIVVGVNRYQDPNEDPLAILKIDPGLERKQIDRLAGVRARRRGEEVETSLARLREA
                     AATDGNLMEPLIECARAHASEGEIVESLQAVFGSYTETPVF"
     gene            38297..38809
                     /locus_tag="VF517_13670"
     CDS             38297..38809
                     /locus_tag="VF517_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054034.1"
                     /translation="MEIDLPEGAVPDPATVTPAGERLERLIDGVITRRVVVQSDERGT
                     VREIFNPAWGFTDEPIVYVYQVTIYPGQKKGWVVHREQDDRLFFDQGAAKLVLYDARA
                     DSPTKGMINELFAGTNNRLLIRIPAGVVHAVVDVGTDEVRFVNLPTRPYNHERPDKMR
                     LPDDAVPYQL"
     gene            38842..39693
                     /locus_tag="VF517_13675"
     CDS             38842..39693
                     /locus_tag="VF517_13675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8054035.1"
                     /translation="MLADEYVRPRPRASDGPLVTVVIPTYNRSEVLRHALRSVLDQSY
                     RRLEVIVAGDACTDDSEEVVASFADPRVTWVNLEVNTGSQGGPNRAALEIANGELVAY
                     LGHDDLWRRDHVALLVADIQRSGADVTYGACDSVFSQGRIAGRRFTCPPLGEPALPSS
                     VMHRRSMCERGAEWRDWRETVNAPDYAFFESLVAAGAKLSRVPALTAVKFPASARRNV
                     YREHRSREQERFARRMNGRAFVAWEILTGLLLLPLRRRTRLPIDQRAYTEPGELVTAL
                     RRVRGLE"
     gene            complement(39704..40498)
                     /locus_tag="VF517_13680"
     CDS             complement(39704..40498)
                     /locus_tag="VF517_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8054036.1"
                     /translation="MSPHPLTSIAAFARRDARVEATHRLPYVIDLLAVAAGVAVFYYL
                     ARFAGRGPGRGDLFPFVVAGLAVLRVNAALPRIVHRFEGEVALGSLAQLLSSRLSSAV
                     VMAGEAAFELLRGCVLGLFLVLLAAGLVEPDLHLGAVAVAGVLIGLAGAAVVSLGLAA
                     GLTGLYLVLRLGLSLSALATIAVPLLAGAYFPIDELPQPLHALATVLPFDDSVDIVRH
                     ALLRGEIDAAALARLAAGAAIVLPLGFWTLHLGTRHSRRVGTLAIP"
     gene            complement(40495..41190)
                     /locus_tag="VF517_13685"
     CDS             complement(40495..41190)
                     /locus_tag="VF517_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8054037.1"
                     /translation="MAAVVELRGATKRFGAVLALDAIDLAVEPGETVVLVGPNGSGKT
                     TLLQLLSGTLEPDAGDVLVCGHDVVRERRAARARTGAVLGGPGGWYPRLSGRANLEFF
                     ASLEALGDAASGAVDAALGAVGLAEAADRRVGGYSTGMRARLALARARLARPPVLLLD
                     EPTAALDARSVREFRDSLRTDDRPPAVLLATHDAEEAVQLGDRVVALRAGRVARELRH
                     DPGLEDVTALMAA"
     gene            41273..41593
                     /locus_tag="VF517_13690"
     CDS             41273..41593
                     /locus_tag="VF517_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054038.1"
                     /translation="MALVALAFPATVAAAKTYTVKVGDDYFSPTKRTVKVNDIVKWVW
                     VAEDGAPGSTVNEHSIVETKDRFHSKTQTAGSYKFRFKKAGKFTIVCGEHPETMIFRV
                     TVKK"
     gene            41607..42020
                     /locus_tag="VF517_13695"
     CDS             41607..42020
                     /locus_tag="VF517_13695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX8054039.1"
                     /translation="MEAGSQRKIRVVVAKPGLDGHDRGAKVIARALRDAGMEVIYTGL
                     HQTPEQIAETVLQEDADAVGLSILSGAHMTLVPKVVRLLNEQGADDVVVTVGGTIPAE
                     DIPELKKLGVAEIFTPGSSTQEIIDFIQGAVGTRT"
     gene            42237..43031
                     /locus_tag="VF517_13700"
     CDS             42237..43031
                     /locus_tag="VF517_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX8054040.1"
                     /translation="MKICVLVKEVPDAAVEKRMDPGTKRLDRSGEKNLNPFDTHAIEA
                     AMQVKEGGVADVEEVIAVTMGPESAVRALHKAVSLGADRSVHLTDPALEDSDVAATGY
                     ALSQVLKREQPDLVLLGQQSDDGECYTIGAVVADHLQMPSLTQVIKMDVQDGKLRCER
                     QAEYGYDTVEVDLPAVISVGDAINEPRYPSLKAIMGAKKKPLESVGTGDVEIETSRVG
                     SEGKRTAVLGINPPPQKEAGQIIEDEDTDDTVEKIVAWLDERKLLA"
     gene            43028..44005
                     /locus_tag="VF517_13705"
     CDS             43028..44005
                     /locus_tag="VF517_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX8054041.1"
                     /translation="MSGILTYALHYQGEFNKNSLGAVTEGARLAKEIGGDAAVVVVGG
                     DDLSDDLCASLGKYGATKVFRANGPEGLGAPVVDVMAKVIQDNDYDYALFGGGLLGFE
                     IGAGLAARLNGGVTMEVTAVKAQDGKLVAERPILQDSAIVDVEYVGKPGIIIGRLNAF
                     EMAEAGEGSASVEDVDVQYSEWSNKARMIERGEQRGAVVNIEDAIILVAGGRGLGTAE
                     GFELCEQLAEALGGAVAATRAVVDAGWYPYAAQIGQTGKTVAPKLYLAAGISGAIQHK
                     VGMQASENIVAINKDQNAPIFEFSDLGIVGDLNKILPKLTEAVKAKRGG"
     gene            44132..45175
                     /locus_tag="VF517_13710"
     CDS             44132..45175
                     /locus_tag="VF517_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX8054042.1"
                     /translation="MVGAGSFGTAVALLLERAEIRTTLLCRTEEQAERMNEARENEKY
                     LPGVQLPDRLKVRTFGGRDDQFHRADLVFVAVPSAGIADAIAELERQGVNDRAGVVSL
                     AKGLVPPDGTPPTEVLDRAFGTERVACVGGPAHAKEMVEGGAGLICASQPPALADTVA
                     SVLREAGVVCEVSTDPVGVELAGCAKNAAALAAGATEPQGLNAAGMAAADIFGEVLAL
                     AEARGGRARTFLGRAGVGDLVAPAMAPKSRNRSAGELLAEGVPARDIPDRLGQAVEAL
                     ETVPLLARAIERAGISAPITSALARLIEGHLPLDDWVALVRVAQPDPERRDRGLRAWL
                     RRIRRRLNGLMRR"
     gene            45199..45414
                     /gene="rpmE"
                     /locus_tag="VF517_13715"
     CDS             45199..45414
                     /gene="rpmE"
                     /locus_tag="VF517_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX8054043.1"
                     /translation="MKADIHPEYVEAHVRCTCGNEFTTRSTKPELHVEICSNCHPFYT
                     GRQKLVDTGGRVERFRRRQARSGRSAS"
     gene            45471..46199
                     /locus_tag="VF517_13720"
     CDS             45471..46199
                     /locus_tag="VF517_13720"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX8054044.1"
                     /translation="MRMRGLALLLTSALSLVALSGTAQAAVPAAKVVDCRTGKAPELR
                     LATFEGRMTAIRGTQRMQMRFELLEQTPGTAEPQPVKAPTLTPWRRSRVGVKAFTYAQ
                     TVKGLASGVTYSSRVYFRWLSPAGKVLRSEQRDSGTCVQDGALPNLVLGSVKSTPGQV
                     EGTAVYTVQVGNTGQGDAESLTVSLIADGAHIDTRTIDGLKAGEFTSVRFTGPYCRRL
                     RAIVDRGATVPETVEEDNELRARC"
     gene            46255..47259
                     /locus_tag="VF517_13725"
     CDS             46255..47259
                     /locus_tag="VF517_13725"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX8054045.1"
                     /translation="MSGTAETAQGMQARKDAPIGGQAVLEGVMMRGVSVWAVAVRKLS
                     AEQVAQGEDRDMREAAAGEIEVVSEPLVSWVKRHRALRLPIVRGVVALAESLKIGFRA
                     LGISADAQAAGHDEEIGKGMWVGTIIFSLALAVGLFFLLPVGLTSLFKDSLPNSLVFV
                     IVEKVIRISIFLGYLWAISKMRELQRVFEYHGAEHKTISNYESGLPLTPENAQQFSRL
                     HPRCGTSFLLIVMIVAIVVFAPLGTPSLPILFASRVIGIPLVAGLAFEVIKLFGRKRD
                     KRWAQILMAPGMQLQKLTTREPDLDQLAVAIAALEAVLAVENPEQATEEDRVGMEVVA
                     "
     gene            47281..48330
                     /gene="prfA"
                     /locus_tag="VF517_13730"
     CDS             47281..48330
                     /gene="prfA"
                     /locus_tag="VF517_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX8054046.1"
                     /translation="MIDDLVGQIESRYRELSAQMSDPEVIGDRERYAEVGRAYSHIEP
                     AYALAVEYRKAADDAAGARELLAEGDDPELRELLRTSEARIAELEEEIRLAMVEPDPA
                     DEKDVIVEVRGGTGGEEAGLFAGDLHRMLARYAERRGFKTEPLSTADGEYTFAIRGRG
                     AYSVFKFEGGTHRVQRVPETESQGRIHTSTATVAVLPEAEEVDVRVDPNDLQIDVYRS
                     SGPGGQSVNTTDSAVRITHKPTGLVVSMQDEKSQLQNREKAMRVLRARLYEQELAARQ
                     AEMSAERKSQVGTGERSEKVRTYNFPQDRVTDHRVKLTKGNLPAVLAGELDELTAALE
                     ADEKRRKLEASSRGE"
     gene            48327..49148
                     /gene="prmC"
                     /locus_tag="VF517_13735"
     CDS             48327..49148
                     /gene="prmC"
                     /locus_tag="VF517_13735"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX8054047.1"
                     /translation="MTTAVGTVRDAIGAAADALGAAGCDTPRLDAELLVAAALGMPRE
                     RLHLEPESGVPPRAARVASEFIRRRARREPVAYILGRRGFRHIDVAVDARVLIPRPET
                     ELLVEVALELPAGARVHDVGTGSGAIALAIADERPDLVVSASDASRAAVDAARANAAR
                     LGLAVAVEHAAGLPETSRDADLVVANLPYVTEAEWAGLAPEIVRYEPREALVSGDDGL
                     DAIRALVAGAPRGLRMALEHAPSQTAAVREMLAEPRTLEDLAGRERVTRGRVLNT"
     gene            49186..49779
                     /locus_tag="VF517_13740"
     CDS             49186..49779
                     /locus_tag="VF517_13740"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX8054048.1"
                     /translation="MEAFQAALLSDGVGLFPADTVYGLAADAESARGVERLYELKGRP
                     PVKPAAVMFFDPDAVLDLARDLGPRTREALARLLPGPVTLIVPNPERRYPLACGPVPE
                     RLGIRVPGLPEPLAALLAIRRPILQSSANPSGGPDPRRLADVDPEIRAGVDAELDGGE
                     LPGTASTVVDLVRYEQAGEFEVLREGAVAAEELAELL"
     gene            50125..51111
                     /locus_tag="VF517_13745"
     CDS             50125..51111
                     /locus_tag="VF517_13745"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX8054049.1"
                     /translation="MLERLAKQICRIAGVELSCIFVRDLRDPRLVIAAAGHGLPWDAI
                     GCRFGADEGPVGHVLATGEPTLSDDFAALVSPLEVPEGSSGCAGVAVPVRWNGRVDGA
                     LVAGCCTPGRAFGEEELLLLSELADLAAAALEHTTERHDFGDLMKAHVEALAAAMDMR
                     DRRTAAHSEDVVRLACEVGRLLGLQDASLVELEFAARLHDVGKIRVPDAVLHKPGPLD
                     EDEFEVIRCHAAWGSETLSGIPGLEAVATVVRFHHERWDGNGYPDKLAGARIPLASRI
                     ICACDAFAAMTADRPYRDAMDNDDALAEICSKSGSQFDPSVVDALAEVVATV"
     gene            51219..52499
                     /gene="glyA"
                     /locus_tag="VF517_13750"
     CDS             51219..52499
                     /gene="glyA"
                     /locus_tag="VF517_13750"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX8054050.1"
                     /translation="MTDLPPDFQTRPVAEVDPEIAQVLEDELRRQDATLEMIASENFV
                     PQAVLDCQGSVLTNKYAEGYPGRRYYGGCEYVDVAEQLAIDRAKALFGADHANVQPHA
                     GAQANNAVYHALLTPGDRVLGMKLDHGGHLSHGMKINVSGKLYDVAAYGVRESDSRLD
                     MEDVARAAYEHRPKLIVAGWSAYPRQLDFAAFRAIADDVGAFLMVDMAHFAGLVAAGL
                     HPSPVPHADVVTTTVHKTLAGPRSGMILCREEHAKKINSAVFPGQQGGPLMHAIAAKA
                     VALRIAASEPFRERQQRTVDGAKRLAEELLGAGVNVLTGGTDVHLILADLRDSELDGQ
                     QAEDRLDEVGITVNRNAVPFDPRPPAISSGLRVGTPALATRGFGDEEFTEVGRIIGTA
                     LTAEWSDDLRTELAERTRGLAERHPLYPQLLQTV"
     gene            52553..53134
                     /locus_tag="VF517_13755"
     CDS             52553..53134
                     /locus_tag="VF517_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8054051.1"
                     /translation="MLRDVVLDIREGVTALLGPSGSGKSTLLRLLNRLADPDRGIVRF
                     RGEDVTMLDPRELRRRACLVPQLPAPLPGTVADNLRYGPALRGRAVGVERPLELAGLT
                     KLYADRDASQLSVGEQQRVMLARALALEPEVLLLDEPTSALDDYTKGGVERTLAELAH
                     RSGLSMVLVTHDRAQAERMAGRIVELHDGAVAR"
     gene            53131..53913
                     /gene="fetB"
                     /locus_tag="VF517_13760"
     CDS             53131..53913
                     /gene="fetB"
                     /locus_tag="VF517_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEX8054052.1"
                     /translation="MRIDVSLLEVLGSLALVALAAGISYWRRIDIERDLGVAVLRSFL
                     QLTAVGFVIQAIFDSDSLLLVALLLAVMVAFGSFTARSRARQVPGALPAIALGLSAAA
                     VITIGLVIGLGVFDAEPRYLVPVGGMVIGNAMTAAAISLNRLADDMRASRLQIEATLA
                     LGATAAEAARAIVQRSLRSGMIGLVDSTKTTGVVFFPGTMVGMLVAGADPVDAVRLQL
                     ILLWTLIGSVAISAVIATSLGYRGFFTPAHQLRELPASRPDA"
     gene            complement(53894..55087)
                     /locus_tag="VF517_13765"
     CDS             complement(53894..55087)
                     /locus_tag="VF517_13765"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX8054053.1"
                     /translation="MPSTGDGFVALGLALVVALLTTPIAGALAWRIGAIDTPRARGLH
                     QMPTPRLGGLAILAAVATSTLVFLPHDQQTNAILIGAAVIVAVGMADDILELSAEYKI
                     VGQILAAAIPVAAGVRVTDFTIPFFGRIDLSDPLSYGLTALGIVALMNVVNFIDGVDG
                     LAAGVCTIAALTFATISLSLDRQVAGVLALIVAGASLGYLRHGFHPASVFLGDSGSNL
                     LGYLLGAIVVQGALKTNAVVALAFPMVILAVPILDSSFVIAKRLKYRQPVYKADRWHF
                     HHRFANIGFSQRKTALYLYGWTLSLAALALALRFVPYSDDHGHFDWEWVAVLAGFGLV
                     AVAASAYLVYVLEILKLKRFRQFQLRNESKLAGAPEPAEADIEARVAHELETGEFEAV
                     KRPGG"
     gene            complement(55094..56986)
                     /gene="asnB"
                     /locus_tag="VF517_13770"
     CDS             complement(55094..56986)
                     /gene="asnB"
                     /locus_tag="VF517_13770"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX8054054.1"
                     /translation="MCGICGQASLDGVTGRDPAALQSMNEALFHRGPDSDGMLLDGPV
                     GLAMRRLSIIDLAGGDQPISNEDGSVHVIQNGEIYNYRELMAGLEQRGHRFSTHSDTE
                     ALVHLYEERGPRFVEDLRGMFAIALWDAGRRRLVLARDRFGIKPLYYATARGVLSFAS
                     ELKALMRQPELSGEVDLDAVEAFLAFNSIPAPLTIYREARKLPPGHLLVWEGGATRVE
                     RYARPGPARAGEQLGGTEDELAEELRERLRDSVRAHLVADVPVGVMLSGGVDSSALAA
                     LASQESSGRVNTFSIGFEERSFNELDLARLVAERYGTEHHELVVRPDAAELMHEIAEF
                     FDEPFADSSALPTYLVSKLASEHVKVALSGEGGDELFGGYFTYVADLIAPVVGRPVAA
                     LRPLVERLPSSSRRTSLDYMAKRFARAGALPPLERHHGWKEIFAPEARAELLGPRRTG
                     VRDPLDVDRARYAETAGSEAIARLQDVDVGIYLPDDLLTKTDRASMAHSLEARVPFLD
                     TALADFALALPRRMRVRGLQKKRLLRKAVAPLVPREIHRGRKRGFSIPAAAWLRGELE
                     PFAREVLSPDTVRSQGFFDPATVTAILDRHVSGREDLSRQLWGLMSFGLWHERHAGAR
                     ALRSKQ"
     gene            complement(56998..57696)
                     /locus_tag="VF517_13775"
     CDS             complement(56998..57696)
                     /locus_tag="VF517_13775"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8054055.1"
                     /translation="MEAATQEAVRPGSLAERYARLAHEANLEPRPVDAVLRGLDVAIA
                     GGTLALTSPLIGSIALAIRLTSGEPVLYRGRRVGRAGRIFTMYKFRTLVPDAEVRLGP
                     YLGNTLSKLTEDEVTAVGRVLRSSQLDELPQLWTVLRGDMSIVGPRPIRPAFFEELCV
                     EIPQYWQRLVVRPGMTGFAQMRLTREHSWAEKLAHDYEYISDRSVHLYMRVVLETLWR
                     IASRPFTGRKALQS"
     gene            complement(57718..58740)
                     /locus_tag="VF517_13780"
     CDS             complement(57718..58740)
                     /locus_tag="VF517_13780"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX8054056.1"
                     /translation="MRIWIDMTAPAHVLVFRPIIERLRARGHEVEVTARDYAQTLELL
                     QMHRIDHTAFGRHGGAARAQKAVALARRTGAMRRFGAGRRFDLALAHGSNDLALAAAT
                     LGIPAANTFDYEFAVQQHNVGCRLARRVMVPDRIPAERLARYGVGPDKLVQYPGLKEE
                     YYLHDFEPDAGVLDQLGVDRDRVVVIFRPPPDVSLYHRKSNPLFPQVLDHLGRHDGVQ
                     AVVIPRTEAQRSYVRELGLPSVIVPEGAVDAQSLIALGDLVISAGGTMNREAVALGTP
                     VYTTYGGRLGGVDEALIRENRLRPLTDPRAIEIEKKDGAGQAQRRDPEVLVDMFLGTL
                     DSSAIR"
     gene            complement(58752..60833)
                     /locus_tag="VF517_13785"
     CDS             complement(58752..60833)
                     /locus_tag="VF517_13785"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEX8054057.1"
                     /translation="MRWDELKVEEQERRALPGFRDPATVRTFDAPEALETRFYEIRAK
                     SAINRVPERSRMPFRWTINPYRGCTHACSYCGAGETPVLMATGRTKLLADLRVGDRIY
                     GTAREGQYRRYAVTEVLDHWKVMRPAFRVTLEDGTVLVTSGDHRFLSNRGWKHVTNTS
                     RSEADRPHLTVNNHLVGTGKFAEPPEDSEDYRRGYLTGVIRGDAHVRHTHRVIGRPVG
                     RTNVFRLALADFEALQRTKRYLADMAVQTAEFEFKVPAGYRPMRAIRATGGPRLETIV
                     NTIDWPAAGSRAWCKGFLAGIFDAEGHFGQCIRIANSDPELINWTAACLDRLGFQHVI
                     EDPRPNGCRNVRIRGGLSEALRFFHTTDPAITRKRSIQGRMVKTFSNLRVASIERLPY
                     MPLYDITTGTGDFIANGVVSHNCFARPTHTFLGLNPGRDFEKEIVVKVNAPELVRREL
                     ARPSWKGEHVAMGTNTDPYQWVEGRYKLMPGIWEAMRDFANPCSILTKSPLLLRDKDL
                     LVQINERAPVSASFSVPTLEEKAWRASEPHTPHPRARLEAVAELNRLGIPTGILIAPL
                     MPGINDDPKQVERIIEIATEAGAVSIGGIGLHLRGEVRDVFMDWLRSYRPDLVDRYEQ
                     LYARGAYLAGPEKERIQSLVRQRSKFTRRNLERDERRAREWRQAAVEREAERRAKEAQ
                     AARAPEQPSLF"
     gene            complement(60974..61237)
                     /locus_tag="VF517_13790"
     CDS             complement(60974..61237)
                     /locus_tag="VF517_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX8054058.1"
                     /translation="MSLIGQVVGEPQLRTSRADVPQCTMRLAIPRRSRHGRREPGVVY
                     VDVTTFGTEAQECADRLREGSTVGLAGRLDDAGHVLIDQLDLL"
     gene            61305..61538
                     /locus_tag="VF517_13795"
     CDS             61305..61538
                     /locus_tag="VF517_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8054059.1"
                     /translation="MARKPDRLLVGLGKAIRELRAERGWSQEELSLKTGVHRNYIGGI
                     ERAERQPTVETVAKLAAALDVTPADLLARAERA"
     gene            complement(61535..>62265)
                     /locus_tag="VF517_13800"
     CDS             complement(61535..>62265)
                     /locus_tag="VF517_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054060.1"
                     /translation="EGSFEVQLILEAAGLWDSVVDLLNKDAADALANLMQIVGGTYGL
                     FAFIRMVKRRLIKGQEQVERGNVRLTLEDGETLEVPTEVFDLYRNVQVRKRARQVVEP
                     LNRRGVERLELRTEEDITVSISSSDVTSFEPPEDIDVPLLERESEMVLAIASVAFIKD
                     NKWRFSDGERTFYANIEDPLFVDRVEKGIEFFRKGDFLECRLRIVQSQQGDALHTDYY
                     VTEVIRHIPRQVQMPLDLPPPDAP"
BASE COUNT         9068 a        22462 c        22217 g         8518 t
ORIGIN      
        1 gtcggcgccg acgtcgtcgt tctcgccggc cggcccgtcg cccggccggt cgtcgagggt
       61 gatgtcgacg ggcctggtgc cgtcgtaggg ggcggcgccg tcgaagccgg ccccgccgcg
      121 aatcaggtcg gccccgggtc cgccgtcgag gaaatcgaag tcgcccgcga acccgtagag
      181 ctcgtcgtcg cccgcgccgc cctcgatgcg atccggcgag tccgtcccga acagccggtc
      241 gtcgccggcg ccgccctcga ggctcgaccg cagccagagg ccgttgcggt ggcgctcgtc
      301 gaggtggagc tcgtcgtcgc cggccccgcc cgtgaggtgg gtgaagcccg gcccgacgtt
      361 gtcgccctcg ccggcctcgc cgtcgttcgg caggccgtcc tggatgaccg tcacggcgcc
      421 cgtgcggtcc gcgtagctca gccgcgagtt cgtaaggccc tgcaacaggt cggcaccggg
      481 accgccgtcc acgtcgacct tcacctgcct ggagatcacc tggtcgtcgc ccggccccgc
      541 gttcacggtc gcctcgagga gaccgagcgt gaggtggtcg tctccgtcgc cgaggtcgaa
      601 ggcggccgag tcgaagtagc cctgctcggc aacgcagacg accgcgttcg ggccgtcgct
      661 gcggcagttc tccccgggag cgagcggtgc gccggtgtcc tcgacgcgga ctccacccgt
      721 cgtcgcgtgg ctcatgctga gacggctgga ctcgccgggc tcggcgcggt actcgaggcg
      781 gtgggcaccg gtgctcggat cgcgcggcgc cgaggccacg ccggcaaacg caggagaggc
      841 ggagacgagc acacatccca cgacgagcag gggcgcgagg cgaatcgaca tcacgccctc
      901 aacctcccgc ctcgccccga gttgcgctgc cggtggcgca ttcgcaccag gcacgccccg
      961 acgctaaagg ccgatacacc agggccgata agcccaacat ggcattcgac ctcgaggctg
     1021 ccctccgccg cgtgatcgag attgacgggt ccgaccttca cctgaaggtg cccgcccgtc
     1081 cgctcatccg caccatgggt gctctggagc ccattccgga caccgacgag ctgacccccg
     1141 aggacaccga gggcgccgtc cgcgagctgc tcaacgaccc ctcgaagctc gaggagttcg
     1201 ccagcgagca cgaggtcgac ttctcctaca gcctccccgg ctgctcgcgc ttccgcgtga
     1261 acgccttccg ccagcgcggc gtcgtctcga tggtcgcccg cgccgtgccg cacgagatca
     1321 agtcgatcga gcagctcggc ctccccgagg tcatcggcca gatcgccgac gaggagcgcg
     1381 ggatcatcct cgtgaccggc accaccggct cgggcaagtc ctccacgctc gccggcatga
     1441 tcgaccagat caaccgcctc cacgcgcggc acatcgtcac gatcgaggac ccgatcgagt
     1501 tcctccaccg cgacaagcgc tcgatcatca accagcgcga ggtcggggcc gacacggcgt
     1561 cgttcaagcg cgccctgcgc cgcgtgctgc gccaggatcc cgacgtgatc ctggtcggcg
     1621 agatgcgcga cgaggagacc gtcagcaccg cgctctccgc cgccgagacc ggccacctcg
     1681 tgctctccac gctccacacg gtggacgcgc ccgagaccgt gaaccgcatc atcgacttct
     1741 tcccgccgca catgaaccag caggtccgcg cgatgctcgc cggcacgctg aaggccgtcg
     1801 tctcgcagcg cctcgtgaag accgccgacg gccgcggccg ggtggcctgc tgcgaggtcc
     1861 tccggatgac cggccgcgtg cgcgacatga tcatgaaccc ggaggagacc gggaagctcg
     1921 tcgacgtgat cgccgagggc gcctactacg gcatgcagac cttcgaccag gcgctgctcg
     1981 cccacgtcca gggcggccgg atcacgatgg aggaggccat gcgctccgcc accagcccgc
     2041 acgacttcaa gctcatggtc gcctccgacg gccagcgctc gacctcggtc gagcagctca
     2101 tgggcgagcc cgagccgtcc gtagtctgac gtcgccgacc tcgcgaatcc gctaacactt
     2161 ctccggcatg cccgtgccgg atcgcatcgc gaatccgtgg ggtgagcgga cgccgctcgc
     2221 gccgggtgac cgctggcccg agcgcgtcga ccagcacctg acggtccccc cggacgacgt
     2281 cgacgcatgg gtgcgcgcgg cttcggtcct ccactccaac ggcgacgcat acgacctggc
     2341 cgtggcggac gggcggatcg tcggagtgcg cgggcggggg gacgaccgcg tcaaccgcgg
     2401 ccggctcggc ccgaaggacc tgttcggctg gcaggcgacc cactcgccgg accggctcac
     2461 gcgaccgctc gtgcggatgg cgagcggcga gctcgagccg gtggactggg acgccgccat
     2521 gggcctgatg gtcgagcgct cgaaggagct gctcgccgag tccggcagcg gggcgttcgg
     2581 cttctacacg accggccagc tcttcctcga ggactactac gcgcaggcgc tcgtcgcgcg
     2641 ggcggggatc gggaccaacc atctcgacgg caacacgcgc ctctgcaccg cgacggcggc
     2701 cgaggcgatg aagcagtcgt tcggctgcga cggccagccc ggctcctacg acgacatcaa
     2761 ccacgccgac acgatcgcgc tctacggcca caacgtggcg gcgacgcagc cggtcctctg
     2821 gatgcgcatg ctcgaccgcc gcgccgggcc cgacccgccg acgctcatcg cggtcgaccc
     2881 gcgcacgacc ccggtcgcgc gcgaggccga catccacctc gctccgaagc cgggtaccaa
     2941 tctcgccctg ctgaacgcgc tcgtccgcga gctgatcgcg aacggttggg tcgaccgcgc
     3001 gtacgtcgac gcgcacaccg tcgggtacga cgacctcgag agcaagacgc gcgagtgcac
     3061 accggagtgg gccgagcgga tcaccggggt cgccgccgcc gacgtccggc gcgcggccga
     3121 ggcgatcggc ggcgccgagc gactggtctc gaccctgctc cagggcgtgt accagtcgca
     3181 ccaggcgacc ggcacggcgg tgcaggtcaa caacctccac ctcctgcgcg gcatgctcgg
     3241 caggcccggc tgcggcgtgc tccagatgaa cggccagccg accgcccaga acacccgcga
     3301 gtgtgggtcc aacggcgacc tccccggctt ccgcaactgg cagaacgagg cgcatgtcga
     3361 ggacctcgcg cgggtctgga acgtggatcc gctccagatc ccgcactggg ccccgcccac
     3421 ccacgcgatg gagatcttcc gctacgccga gcagggctcg atccggttcc tgtggatcac
     3481 cggcacgaac ccggcggtct cgctgccgga gctggagcgc atccgcgcga tcctccgcca
     3541 ggagcgcctc ttcgtggtgg tccaggacct cttcctgacc gagacggccg agctggccga
     3601 cgtggtgctg ccggcggcga cgctggggga gaagaccggc acgttcacga acgcggagcg
     3661 gaccgtgcac ctggccgaga aggcggtcga cccgccgggc gaggcgcgca ccgacctcga
     3721 gatcttcctc gactacgccc gccggatggg cttcaccgac cgcgacggca agcctctcat
     3781 caagttccac gaccccgaga gcgcgttcga ggcctggaag gagtgctgcc gcggtcgccc
     3841 ctgcgactac accgggatca cctacgcgcg gctgcgcgag cgcagcggga tccagtggcc
     3901 gtgcaacgag gacgctcccg acggcaccga gcgcctctac accgacgggc gcttcttcgc
     3961 gtcccccgag gagtgcgaga gctacggcaa ggacctgctc acgggggccg cgctcgagga
     4021 gaccgagtac cgcgccgcga accccgaggg ccgcgcgatg ctaaaggcgt gcgagtacgt
     4081 gaagccggtc gaggcgccgg acgacgagta cccgttggcc ctcacgaccg ggcgcacgat
     4141 ctaccacttc cacacgcgca cgaagaccgg ccgcgctccg gagctgcacg cggcggcgcc
     4201 cgagccgtgg gtcgagatgg ctccgagtga cgcggaggcc gccggcgtgg cggagggcga
     4261 cgaggtcgag gtcgtgacgc cgcgcggccg ggtgcgggtg ccggtgcggc tcacggggat
     4321 gctgccgggc gtcgtgttcg tgccgttcca ctacggctac tgggacgctc ccggcgccga
     4381 ccacgaccgc gccgccaacg agctgacgat caccgcctgg gacccggcct cgaagcagcc
     4441 gctgctcaag gtgggcaagg cgcgggtgca gcgggcgtga agctcgggct cgcgatcgag
     4501 cagctcgccg agtccgagga gcggctcgcg accgagctgg cgcgggcggg cgagcgccac
     4561 gaggccgacc acgacgtgta ccacctgagc tccaccctgg cggggatctc gcgcgcgcac
     4621 ctgaaggcgc tggcgccgtt cgcggagcgc tacggggtcg ccgtcgagac gaacggcgcc
     4681 ggtccggcgt ccgggctgct gggcgcggtt cgcgagaggg cgtccgagct caccggccgg
     4741 cggcccgagg cggggctgct cctgctgcgc gacctgcgcg agctgtacct gctcgcgtcc
     4801 gacgcctcgc tcggctggac cgtcctcggc cagggcgcgc aggccgcccg cgacagcgag
     4861 ctgctggacg tggtgagcga gtgccatccg gacacgctgc gccagatcaa gtggatcacc
     4921 acgcggatca agcaggccgc gccccaggcg ctcacgacct aggcgccgcc gccccttgac
     4981 gcgcggcggc gttgccgcaa gtgtttcctc tccccccacc ccgactccat cagggaggca
     5041 gtacatgcag agcactcttg ggcagttcca ggagaccgag tcgaccgagc cgttcagcct
     5101 tcagaacagc aagctgctga aggtcgagct gaaccaggtc acgatccagg cgaagctcgg
     5161 ttcgatggtc gcctaccagg gcgacgtgac cttcgagcac gccggctccg gcggcatggg
     5221 ccggatgctg aagaaggcgg tcaccggcga ggggaccaag ctgatgaaga tgtcgggcgc
     5281 cggcgaggtc ttcctggccg accaggccca ggacatccac ctggtccagc tcgagaacga
     5341 ctacatcacc tgcaacggcg ccaacgtcct ggcgttcgac gcggggatcg actgggacat
     5401 caagaaggtg gagggcggcg tggccgcggc gatggccggc ggcctcacga acatgaccct
     5461 ccaggggacc ggctgggtgg cgctgatctc ggacggcccg cccgtcctgc tcgacgtgtc
     5521 gacggcgccc acgttcgccg acgcccaggc cgcgatcacg tggtcgagcg gcgtccagac
     5581 gcaggtcaag gtcgacgcga acctgaagtc gctgatcggc cgcggctcgg gcgagagcat
     5641 ccagatggcc ttcagcggcc agggctgggt gctcgtgcag ccgagcgagg gcatggtcca
     5701 ggcggctgcc gccggaggtg gcggcggtgg cgccgcgggc ggcctcgccg ggatgctcgg
     5761 cgggcgctag ccggaccggg tgccggctcc cgccgcgggg gccggcaccg cttagccgct
     5821 ggggttgcac cgcttccgcc gctacacttc actttgtgaa gcaagggagc gacgtctgtc
     5881 ccgtctgtgc gaccgccgag ctcgtctgca ccaagtggac gatgctcatc gtccgcgatc
     5941 tcgcacaggg ccgcagccgc ttctgcgagt tcgagcgctc gctcgccggg atcagcccgc
     6001 gcacgctgtc gctgcggctg cgtgagctgg aggccgaggg cgtggtcgag cgccgcacct
     6061 acccggaggt cccgccgcgg gtcgagtacg cgctcaccga gaagggtcag gcgctcctgc
     6121 cgatcatcga ggtgatgcgg gacttcgggc gcgagtggct cgacgcccac gggccgtgcg
     6181 acgtgccgga cgaggcacgc gctcccgccg cggtcgccta gccgcacccc ttcgcaggag
     6241 cacgcggcgc cgcggcgaaa tggcgcctca tgcgcaaccg cgagctgcac gacgcactcc
     6301 gtgacttcgc gctcgagtcc gccgcgctgc tcaccgcggc ggtcggcgcg ggcgccgagc
     6361 tgccctacga cgttctcgag gagccgggca caggctcggt cctctaccgc taccagccgc
     6421 tgacggccga gttcatcggc gcgcgctggg actcgatctc cgcccagtcg agcttcgagc
     6481 gcgcggcgcg cgccctcggc accggagccg aggcctacct gcgcatgcgc gggctgcccg
     6541 gcggcgcgga ctccgagccg gcgctgcggg cgctgctcga gcgcctctac gaggacgcga
     6601 ccggcttcga gttccccgag gagcgcttcg agcgcgtgta cgcggagttc gagcgcacgc
     6661 tctacgagca cacgctgcgc gcatcggtgg tcgcgccggt gcacgggctg gtgctcgagc
     6721 gcgagcgggt ggagctcggc ggcgggctga tcctcgcgcg cggcgatcgc gtcgcggctc
     6781 ctccggaggc ggtctggccg ctcgggccgg gggagcgcga ccgggcgacg ggccccaaca
     6841 ccgtggtcgc gctcgagcgc gacgtggagg ccgacgccgg catgcccgcc ggcgaggcac
     6901 ggatccggtt tcgcaggctg ctcaccgccc tgcggctgct gaagcccggg cgcgtggcgt
     6961 tcggcgctcc cgcgtggggg cgggccgacg agggcgcctg gcagccgatc cctctgtcgt
     7021 tcggaggccg cggccgcggc gagccgtggc tgctgcgcgc gtccgagggc ggggagctgg
     7081 ccgagctggt cgagctcgtc tcggcggcgc ggctgccggg gcggatcgcg tgggccctcg
     7141 ggcgcttcga gatggggtgc gagcgcggct ccgacgccga ggcgctgtcc gactacctgc
     7201 tggcgctgcg ggcgatgctc gacgccgacg acgagaccgg ccgcgcgagc ctcgggttgc
     7261 ggctggcggc gctgtgcgcc gaggagtccg agcggcgcgg ggtgcgcgcg cggctcgaga
     7321 cggcgttcgc gctggagcgc tcggccatgg gcggcgccgt gctcgacccc gacgacacgg
     7381 ctcccggcgc ggtcgtgcac gagctcgagc agcacgtgcg cgcgctgctg cgcgacgtgg
     7441 tgtgcggcta cctgggcggc gacctccgct cggtggccga cgacatcctg ctcgcgagcg
     7501 ccgacccgat cgagatccgc gcacgcgacg tgcgtgccga gccggacgag gtccccgacc
     7561 tgcgcgaccc ggcgcccgac ctgctcgacg agccgccgcc gcgcgccgag gagcgcgagg
     7621 aggggttccc ggagccgctg gcgacggggg agcacgaccc cggcctcttc gcgcgcacgg
     7681 gggacgaaga ctgggaggag ccggacgatg cggcggagcc cgatgccgac ggcggtccgc
     7741 tgctgcggcg cgtgcgccgc ggcgacgggc ccggcaccgc cgcggacccg cgctcggcgg
     7801 cgtggcggat cgcttcgcag ccggggccgc gaagggggcg cgagcggccc gagcctgcgg
     7861 cgacgccggc gtcggtgatg gcggacgagg agctgacgat cgccgaccgc cccatggcgg
     7921 cggcggtggc ggagccgccg gtggcggagc cgcggcgcgc cgagccgagg agcgccgagc
     7981 cgccgcgcgc cgagccgacg gtggcggagc ctcgcgccga gccgaggagc gccgagccgc
     8041 cggtggcgga gcctcgcgcc gagccgagga gcgccgagcc gccggacccc cttgcggccg
     8101 ccgcgccgga gccgctgctt ccccccgagg agcccacctc acggatcgcc gcgctcgacg
     8161 cgcccgagat cgacgtcgat cacacgatca gcgcgggcgt caccccgtcg gcggactggg
     8221 acgacgacga ctgctggtcg gcgccggtct gaccaccgaa gcgccggaaa cggccttcgc
     8281 cgagctggcc gctgcgctca cgcgccagcg cggcgtgacc gaggggaagg cgttccgctc
     8341 gcccggcctg aaggtcgacg ggcggatctt cgcgatgctg gtcggggagg agcttgtggt
     8401 gaagctcccg gccgagcgct gcaaagccct ggtcaccgac ggcgccgcgc ggccgttcga
     8461 gtcgggctcg cgcaagatgc gcgagtgggt cgtgatcgag ctgccgccaa cgcggtggcc
     8521 ggagctcgcg cgcgaggccc tgtccttcgt acgctaggcc gtcaccgcgt ccgccgcgaa
     8581 caggtagtcg ttcacggcgg cgaggcccgc gagatcgtgc acgtcgtcat cgagcagcgg
     8641 cacccgcacc agcgggtcgc cgtccagctc gcgctcgagg cgcgcgatgt tgtccgcgtc
     8701 gcgcgcggcc agctcgcggt agtcgtcgaa gttgcgcgcg accttgcggc cgagcttctc
     8761 gccgagcagc gcacccaggt cgggctcgac gtcggcgccg ctgacgtcgg tgtccgcgcc
     8821 ctcgtgcacc cggttcacga tcgccgcccc gaacggcatc ccgttctcgc gcaggcggcg
     8881 gcggaagtag acgacgtcgt ccaccgcgtc gcggcgcggg gaggtgatga gcaggaaagt
     8941 cgtgtccgcg tcctcgagca ggctctcgac caccttcgcg cggtcgcgga tcccgatcgc
     9001 catgtcgccg aagctcgcga agaactccga caggtcgcgc agcaggtcca cgccggtgag
     9061 ccgcttgagc agcgagaaga gcacgcccgt cccgcgcccg aacgccttca cgccgaacag
     9121 tcccggcttg aggaagaacg acagcgagcg cgagtcgatg aagcgtgtga gccggtcggg
     9181 ggcgtcgagg aaatccaaag cgttgcgggt gggcggggtg tcgagcacca gcaggtcgta
     9241 gtcgcccgac tcgtgcagct cgtagagctt ctcgatcgcc atgtactcgg tggacccggc
     9301 cagcgcgttc gagagctcct ggtagatccg gttgccgagc accgcgtcgc gggtggcgtc
     9361 gtcgggcgcg cgcgtctcga tcagctcgtc ccaggtgcgc ttggcgtcga gcatcatcgc
     9421 ccacagctcg ccgcgcatct cgagcccgtg ttcctcgaac agggccggat cgatccgggt
     9481 ggcctcgttg ccgagctccg acaggccgag cgagttcgcg agccgccgcg ccgggtcgat
     9541 cgtgagcacc gccaccctca gcccctgcgc ggccatgccc atcgccacgg cggccgccgt
     9601 ggtcgtcttg ccgacgccgc ctgacccggc gcagatgcag acgtgcttgc cgtcgagcag
     9661 ctcgccgacg ctcacaggcg ccgctccagc tcgcccgaca ggccctccca gtcgtcgatg
     9721 tcgaggtcgg tgtcgaacag gaacggcagc gtgaggaccg gcgcgccgtc gcggcccttg
     9781 cgcagccgcc ggagctgcgc gcgctgggcc cgggagcggt ggtgctggga cagggcggtc
     9841 cggagggcgg cggcgacggt cggatccccg gcgccgccgt tcacggcctc gatcgcggcg
     9901 gcgtcctcac cgccgaagcg ctccggcagc atcgcgttca tcaccacgag cccgacgtcg
     9961 tagcccatct ggtcgcgcag cttcgtgcgg aactcgagcg tctcgttgac gggcatctcc
    10021 tcgggcagcg ctacggccac gacgccggtc tgcgccgggt cacgcaggaa gccgtcgatc
    10081 atcagcgcct tgcggcggat cggcccgacg cgcgccacgt cggcgaacgt ctgcggcgtc
    10141 ttcagcatgc cgagcccctg gcccgtgggc ggtccgtcga ggatcacgag atcgtacggc
    10201 gcgtcgccgc ggcggcggcg ctcgagcagc gccagctccc agaccttggc gatcgtcacg
    10261 agctcgcgca tccccggcgt ggccgcggcg aagtactcga agatgcggtt gttgaagagg
    10321 agcccggcca gccgcttcga cccgacctgg tccacgagga actcctcgag cgacttgtcg
    10381 gggtcgatcg agatgccgta gaggttgggc gcgagctcgg tctccgcgta gccggcgccg
    10441 gcgtggcgga acgagcgcga gacccgctcc tgcttggcca cctcgaccac gatcgtgcgc
    10501 ttgccacggc gcgccgcaag cagcccgagc gaggccgcga ccgtggttcg gccgacgccg
    10561 ccctttccgg cgacgaacag caggcgcttg tctagtaggt cggacacggt gaaggattct
    10621 tctctacccg tacgaatacg cacggcacat gggactcttc gatcgaaagc ctgacgcacc
    10681 gcagtcctcg gcggctcccc cgccgcccga cccgtcgagc atgcaaccgg cgccagtcgt
    10741 ggaaccgaag gtcgccgagg tgatcgtatt cgccaaccag aagggcggcg tggcgaagac
    10801 cacgacgacc ctgaacctgg cgtgcgcgtt cgcggagaag ggccaccgcg tcctctgcgt
    10861 ggacatggac ccgcagggca acctgacgat gagccaggga atcgaccccg actcgctcga
    10921 gtcgtcgatg tacgacgtgc tcgtccacca catccccatc cgcgacgtca tccgcaagcg
    10981 cgagatcgac gtggcctgcg cctcgatcga cctggccgga gccgagatcg cgatgtccac
    11041 ccagatcggc cgcgagcgct cgctggagaa ggcgttcgca gggatcaagg aggactacga
    11101 ctacatctgc atcgacacgc cgccctcgct cgggctgctc acgatcaacg ccctcacggc
    11161 ggccgacaag gtgatcgtcc ccgtccagtg cgagtatctg tccatgaggg ggctgatcca
    11221 gctccagaac accctgtcga tgatccgcga caacctgaac ccgaaggtcc gtatcgaggg
    11281 catcgtgccc acgctggtcg actcccgcac gacccatgcg aaggaagcga tcgagctgct
    11341 cgaggagaac ttcggggacc gggtgtacgc gtcgaggatc acgaagaccg tgcgcttcgc
    11401 cgaggcgccg gtcaagggca tgtccgtgct caagtacgac cctgatggaa aggcggcgta
    11461 tgcctaccga caactcgccc gagaggtcct cgcgaatgga tcgcggtaag cgcgcgagca
    11521 tgcgcgaggg ccccctcgcg cagctcttcc gccgcaccga cgaggacgcg ccggagccgg
    11581 gtcgcgagca ggcgcccgcg gagccgcagg ccgcgccccc cgaggagcgc gcagcgcagt
    11641 ccgcgcacga gcgcctggac gggccgatcg cgcgcgccga gacccacgac gagccgcgca
    11701 cgccggacca ggagcgcgcc gcgcgccgcg aggccgagcg ccgctcgccg gtcgaggggc
    11761 tcgaggacct gcgtcccgcg ccgccctcgg cacgtcagca tcacgagccc gagccgcggc
    11821 gcgagccggt cgagcccccg tcgcccacca cgccgcaccg cgagccgggc gtacggccgc
    11881 cgaagcagcg gctcgaggag gtcttctcca acgaagtgcc ggacgacatg ctcgcgcgca
    11941 ccccgcgtcg cgacgacgag taccgcggcc gctacggccg cgacgtgccg gagccgacgg
    12001 cggtcacgcc agagccggtg ctccagcccg tgctgcgcgt cgtgggcgtc ggcggtgccg
    12061 gcgtcaacgc cgtcaaccgg atgatcgagg ccggcgtcga gggcgtcgac ttcatcgcgg
    12121 tcaacaccga tgtccagtcg ctccagcagt cgagcgcgca catcacccag cacatcggga
    12181 gcgacgtcac gcgtgggctc ggctccgggt cggatcccga gatcggccgc caggcggcgc
    12241 tcgactccta cgacgacgtc aaggcgctgc tcaggggctc ggacatggtg ttcatcgccg
    12301 ccggctcagg tggcggcacg ggtacggggg ccgctccggt ggtcgcgcgc tgtgcgcgcg
    12361 agctcggtgc gctcaccgtg gggatcgtca ccaagccgtt cacgttcgag ggctcgcgcc
    12421 gggccgacca ggccgagcgc gggatcgagg agttggccag cgaggtcgac accctgatca
    12481 cgatcccgaa cacgcgcctg ctgtcggtgc tcgacaagcg cgtgtcgatg gtcgacgcgt
    12541 tccgcatggc cgacgacgtg ctgcgccagg gcgtccaggg catctcggac ctgatcacgc
    12601 tgcccggcct catcaacctc gacttcgccg acgtgcgcac gatcatggcc aacgccgggc
    12661 cggcgctcct gggcatcggc atgggcgtcg gtgagcagcg cgcgatggag gcggccaccc
    12721 acgcggtcgc gtcacccctg ctcgagacct cggtcgaggg cgcgcggtcg atcctgctgt
    12781 cgatcaccgg cgggcgcgac ctgtcgctgt gggaggtcaa cgaggctgcc aaggccgtcg
    12841 ccgaggccgc tcacccggac gccaacatca tcttcggggc gatggtcgac gagaacgtcg
    12901 tggaccaggt ctgggtcacg gtggtggcga ccggctacgg cgagcgtccg gcccggcgtg
    12961 cctcctcggg gcgcgagggc gggcgggcgt cgtgggagcc ggagggcgag ccgcgcgtca
    13021 cgcgcggggc tggccgtagc cagaacggct ccgcgctgag cgacatcgac attcccgagt
    13081 tcctcccgcg cagctagccc agggctagca tcgccccgat gggaggggtg gtcgcagcgg
    13141 ggcacccgct gaccgctgag gcaggagcgc gcgtgctccg cgccggcggc aacgcggtcg
    13201 acgccgccgt ggcgggcgtc ctgatgtcgt tcgtcaccga gtcgccgctc accggcccgg
    13261 gcgcgggcgg cttcatgctg gtccacaccg ccggcgggga ggaccacctg ctcgacttct
    13321 tcgtggcggc gcccggccgc gggctcgccg agggcaccga gccggccgcc ctgcggccga
    13381 tcgagctgcg cttctcggag gaggcggtgc agacgttcaa cgtcggcgcc tcgtcggtcg
    13441 gcgtctacgg gaccacggtg gggatcgccc aggcgctgcg gcgcttcggc accgtccggc
    13501 tcggcgacct caccggcttc gcggcccagg tcgcgcgcga cgggcacgag gtcacgccga
    13561 tggaggggtc gttcatcgag atcctcggac cgatcctcac ctccacgccc gaggccgcgg
    13621 cggccttcgc cccggacggc cgggtgccgg tgccgggcga cacgctgcgg ctgcccgagc
    13681 tcggcgacct gatcgagcgc ctcggcgccg agggacccgg cttcctctac gtcggcgacg
    13741 tggccgaggc ggtctgcgag tgggtgggag agcgcggcgg gctgctcacg ctcgaggatc
    13801 tgcgctccta ccaggtgatc gagcgcgagc ccgcgcgcgc cgagtaccgc ggccgcacga
    13861 tgctcacgaa cccgccgccg tcgtcgggcg ggatcctgat cgcgtactcg ctcggcctgc
    13921 tcgagcgcct cggccggccc gcggatgtgg gcacggtggt cgaggtgatg gaccaggcga
    13981 accgcgcgcg caccgacgag ttcctccagg gcctgcacgc ggagggctac ctggagcgct
    14041 tcctggcgaa ggacgcgctc gagaaggcgg cctcgcggct cggctcgaca acccacatct
    14101 cggtgctgga cgacgacggc ggctgcgcgg cggtcacgtg ctcgaacggg tcctcgtccg
    14161 gcgtgttcgt ccccggcacc gggatgcatc tcaacaacat gctgggggag gaggacctga
    14221 acccgttcgg cttccaccag cacgcgcccg ggcgccggat cccgagcatg atggccccga
    14281 ccgtcgtgct acgcgagggc cacccccaga tcgccctcgg ctcggccggc tcgaaccgca
    14341 tccgctcggc catcctccag acgatcctgc gcgtgatcga cgacggcatg cccgcccagg
    14401 aggcggtgac gcacccgcgc gtgcactggg agggccgcgt ggtggaggcc gagcccggtg
    14461 ttgacgagga ggccctggcc gctttggagg ccggcggccg cgaggtgatc cgcttcgccg
    14521 agcagagcct gtacttcggc ggcgtgcagg cggtggcccg cgaccccgag accggagccc
    14581 tcagcggggg cggcgacccc cgccgcggcg gcgcggcggt ggtcgttccg tagggcattc
    14641 ctgacttatg ggtctatacg acccacaagt caggaatgcc taagaaaaag ccccggcgag
    14701 cccatcgcca caatccatcc atgctcccta agtgcaagcc gttcggaggc gccgcgctgg
    14761 ccgccacgac cgcgctgacc gcgctggcct gcggcaaccc cggcggcgac ctgatcgcgc
    14821 tcgaggagcg cggcgcgcac ttcacgcgcc tagtcgtgac ggacgacggg cgcgggagct
    14881 gcaacggcgg tgagctgaag cgcctggaga gcagccgcct gctcgaagcg cgcgaggtcg
    14941 agcgtgagct ccaggagctc gcgaccgagc gcacgaactt cccggacccc accggcgggc
    15001 gccagttccg ggcgaagatg cgcgacgcga ccgtgacgtg gatggagcgc tcccagcagg
    15061 atcccgtcct gccgaaggcc acggcgctga cgctcgagct ccggcgcgag ctctgccccg
    15121 cgccggcgac tccctagaag cgcaccttgc cgttcgactt ggcccgctcc tgggcctcct
    15181 cggcgttgtc gatccggtcg agggcggtca cgaacaggtc gcccacggtc ttcaggcgcc
    15241 tgtcctcgga gcgctcctcg agcagcgcct gcttcggggc agcagccagc tcgatggtcg
    15301 cggccatccc gtacgccgtc agcccctcga gctcctcgat cccgggacgg ttgtcggtca
    15361 cgcgctcgac caggtccgcg tacgcctcac gagcgcccgc cgcgagctcc tcgtcgccct
    15421 cgtcgaggtc ctcgatcatc tccacgtcgc cggccgggta gggcatgtcg tcgatcttgc
    15481 gcaggagccg gaacgggcgc ccgccctggg cgaggatgtt catgcggccg tcgtccatct
    15541 gctcgaggac ctgcgccacg cgcgcggtgc agccgacctc ggccagaccg tcgtcgccga
    15601 gccagacgat cccgaactcg ccgccgctct cgaccgactc cccgatcatc agcttgtagc
    15661 gcgtctcgaa gatgtgcagg ggcacgatct ccgtcggcag cagcacgatg ccgagcggga
    15721 acaggggaaa gcgctcctcc acatgggcca tacggggagc ctacggggct tggttcacct
    15781 cacgacttcg ggaactccat cacgaacgcg gagacccggt cgttctggtc ggtgatcgtg
    15841 ttgggctgcc cgaccaggct gagcgtgtgg ctgcccgccg ggatgttggg cacgaccagg
    15901 tcgttcgtga ccgcggccat gtgcacgctg gtcgggttgg cgaagatccg gagcatcccg
    15961 accggggtgc tgtcgatcac gacgctcatc acgagcggcg cgttcgccgt ctgcgtccag
    16021 cacgacgcgg cgcccttcac cagcagcgtg ccgcccttgc tcgtgaccgg caccgacgcg
    16081 atcgtccccg agccctgctg tgcctgggcg gcggcgttcg tgatcccggc gaggtacgac
    16141 ggcgcctgcg cgtaatccac ccactcgacc acgctcaggt gcgcgtagtc gttcgggtcg
    16201 gtctgggtct ggccgccgcc gaagacctgg aactcgtgat tgccccggct ggtgtcctcg
    16261 acgttgtccg tcggcacgac cgtcaggcgc tggttggcgt tgttggcgaa gacctccatg
    16321 ggcgcgccgt cgccgccgtc gtagaggagc gccgagcgga ccgtcgtcgc agcctgcgtg
    16381 acccagccgg agctcgagcc gctgatccac acgccgccgc ccttggtctc ggctctggcc
    16441 gtgatgagcg gctgcgccac gccggtcggg cacggtgcgt cctccgcgag ccggatcgca
    16501 agcccgtcgt tcggctcgat gaccgtcagg catacgacgt cgttcgggtc cgtgacggtc
    16561 gccgggccgg tctccagcat gatcacgtgc tggccgggcg tcaggccgtg aacccacgcc
    16621 cacgtgtggc cgagcgagag gtgcatcgcc ccctggctgg cgtaggtccc gagcatcgcg
    16681 ccggtgggct ggccgtcgag ccagaggctc atagagatcg gcccgggctg ccccgaccag
    16741 gcggaggcgg agaacccgat gagcgcgtcg cccccggtga aattcaccgg ctgcttgatg
    16801 acgaagccgc cggtctgggc cggtagcgcg gcgttgttca gcggctggga aatcgtgctt
    16861 gaggtcatcg cgcgacgcta accgggttca tccgccgagg caagggcgca cgtatccgcg
    16921 gcgatgacgg cgatcgtctg gttccggcgc gacctgcgcg tgcgcgacca ccccgcgctc
    16981 cggcgcgcga tcgccgagca cgaccggatc gtcccgttct tctgcctcga cccgcggctg
    17041 ctgcacggcc gccacgcctc cggcccccgc acgcagttca tgctcgagtg cctcgccgac
    17101 ctggacgagt cgctcgacgg ccggctcgtg gtccggcacg gacggcccga ggaggagctc
    17161 gtgcgaatcg cgcgcgaggc gggcgccggg gacgtctact tcagtgccga cgtgagtccc
    17221 ttcgcgcggc agcgggggag ccgagtcaat gaggcgggcc tgcgcctcca cgcctgcccg
    17281 ggcctcaccg tggtcgacga cgtcggcgcc ctccagaccg gcaccggccg cccctacacc
    17341 gtgttctccc cgttccatcg ccgctggtcc ggcgagccgc gccgcgccgt gctgcgcgcc
    17401 ccccgcaagc tcgccggccc gagcctctcc ggcgcgatcc cgtcgctgac cgagctgggc
    17461 ctgcgccagg aggtagagga gccgatgccc ggcggcgaga ccgcggcccg ccggcggctc
    17521 gccgccttct cgatcgagga ctactcgcgg gaggacctcg agagctcgtc gcggctctcg
    17581 ccgtacctgc acttcggctg cgtctcgccg ctcgaggtcg agacgcgcga gccgggcctc
    17641 ggccgccagc tcgcctggcg cgacttctac caccacgtca tcctgcgctt cccgcgcaac
    17701 gccgtgcagg agcaccagga gcgctaccgc ggctcgctcg cctgggacca cgacggcgag
    17761 ctgctgagcg cctggcagga gggccgcacc ggctacccgc tcgtcgacgc cggcatgcgc
    17821 cagctccgcc gcgagggctg gatgcacaac cgcgcgcggc tcgtggtcgg ctcgttcctg
    17881 accaagcacc tcgggctcga ctggcgcctc ggcgagcgct ggttcatgcg actgctgatc
    17941 gacggcgacg aggccaacaa caacggcaac tggcagtgga tcgcctcggt gggggtcgac
    18001 ccgcagccgg cctaccggcg cctctacaac ccggcgcgcc acctggagcg cttcgacccc
    18061 gacggcacct acgtgcgccg ctacgtgccg gagctacagc gggtgccgat gacccacctg
    18121 cccgagccgt ggaagatgcc gccggagcta cagcgccagg ccggctgcac gatcggccgc
    18181 gactacccgg cgccgatcgt cgatcaccgc gaggcgcgcg cggcggcgct cgagcggtac
    18241 cggtccgccg cggcggccgc ctgaggctcc gccggcgcct gcgccgagac gagcaggttg
    18301 tagaagatcg ccggcggcag ccgcccctcg agcagccgtg cgtccacctg cttgaggccc
    18361 aggtagccga agtacgcgta gcggatccag gcgagcggga tgctgtccgg ctcggcggtt
    18421 gactcgagcg tccggttcat ccagccgaac aggccggcca cgagctcctc gccgcgcacc
    18481 cgcacgccct cgaacccggc gccggtggcc agctcctcga gctgcccggg ggtgaatgcg
    18541 tggacgtcca caacgtgctc gagcgcgtgg tcgtgcccgt cgctctcggg caggccccgc
    18601 tcgcgggcgc cgaggacccg gcgccaggcc ggggcgagcg ccatcgcggt gcgcttgggc
    18661 acgcccgcga tccggtcgcc gtggcgcgag ggctcgccgc agaacgcgat ccgcccgccg
    18721 ggccgcagca cgcggcggaa ctcccggaac gccgcgtcga ggtcgggcag gtggtggagg
    18781 atcgcgtgcc cgaagacgag gtcgaacgag tcgtcgggga acggcagcgc gtcggcctcg
    18841 ctgcacgcgg tctccacctg cacgccgagc cgcctcgccg agtcctccag ctcgcgcagc
    18901 atcccgggcg agatgtcggt ggccacggcg gagtcgatca tcccggcgcg cagcaggttc
    18961 agcgtgaagt agccggtgcc ggcgccgatc tcgagcgcgc ggccgaagcg ccggtcggcg
    19021 ccgagcgcgc ggcgcatctt gccgaccacc tgcgcgcgcc cgtcgggacc gtaggagatc
    19081 ccccacttgg agtcgtagtc cgcggccgcc acgtcgtggt agcgcacgtt cacttccctg
    19141 atctcctcgt gtgagacggg cggcacggcg cgcgactata tgcgcgaccg tcgctatcgt
    19201 cgcgccgccg tgtccgagcc tgcgagctac acgcagcagg accagccgcc gggggtcccg
    19261 ccgctcagtc tgacgggaga gcggacgctt ccggacgtcc cggaggagaa ctactggtac
    19321 cgccgccacc tcgcggtcta ccgctgggtg gccgagcgct gccgcggcgt cgacgtggtg
    19381 gacatggcct gcggcgaggg ctacggcacc gcggtgctgg ccgagcgcgc cgcccgggtg
    19441 accggcgtgg acgcgaatcc cgaggcctac gagcacgccc gcacgaagta cacgcgcccc
    19501 ggcgtgcgct tcgtgcgcga cctcgtcgag ggctactccg agccctgcga cgccgtcgtc
    19561 ttcctccaga cgatcgagca cctcgaggac ccgaaggccg tgctcgacca cttccgctcg
    19621 atcctgcgcc cgggcggcgc ggcgtacgtc tccacgccca acctgctcac gctcgcgccc
    19681 cccggcgcgg agaagtcgga caacccctgg cacgtgcggg agttccgcgc cgaggagttc
    19741 cgcgagctgt gcgaggcgag cttcgagcgc gtcgagctgc tcgggctctt ccacgcgcgc
    19801 aagctccgcg cgcacgagct ggcgatcagc cacggctggg acgacctgca cccgaagctc
    19861 ggcatcacca agcgcttcta cgactggttc acgcccgcga tctcggagca cgacttcgcg
    19921 ctgcggccgg ccccgctcga tggggcgctc gacttcgtcg cggtcctgcg atgagcgcgc
    19981 tgcgcgcgga ccccgatcgc gggcagctag cgatcgtcct ccacacacac atgccgtacg
    20041 tcgagggctt cggcacctgg ccgttcggcg aggagtggct gtgggaggcg ctcacgggcg
    20101 tctacctgcc gctgctcgac gtgctgcgcg acgcgccggt cacgctcagc ctcacgccgg
    20161 tcctgtgcga ccagctcgag gcgctcgagg gcgaggcggg cgagcgctac ctcagcttca
    20221 tgcgcgagat gcgcgccccg atccacgcag aggacgcgcg cgggctcgac agcgtgggcg
    20281 cgcacgacgc cgccaacgag ctgcgccgcg cggccgccga ctaccgcgag ggcgagcgcc
    20341 gcttcaccga gctgcgcggc cggctcctcc acgagctgcg cgagctcgac cgccactcgc
    20401 ccgtggagct gtggacgagc gcggccaccc actccgtgct gccgctgctc gcgaccgacg
    20461 ccggcctggg cctccaggtg agcaccggca tccgctcgca cgagcgccgc ttcgacgaat
    20521 ggagcggagg cttctggctg cccgaatgcg cgtaccgccc cgggctcgag cgcgacctgg
    20581 ccgagcacgg cgtgcgcgcg ttctgcgtgg accagaccga gtcgctgggg ctcggctcgc
    20641 tcgaccagct cgagccggtg cggaccgagc ccggcccggt cgccgtgccg atcgactggg
    20701 agctgacgaa gctcgtgtgg gactccgccg ggtatccgtc agccgcgctc taccgcgact
    20761 atcacggccg caccacccac gacctgcgcc cgtgggacaa cggcggcgag ccctaccgcc
    20821 actggcaggc gctcgtgctc gcccgccgcc acgcgcgcga gttcgtcgcg cacgccgccg
    20881 ggcggctcga cgcctaccgc gccgagcgcg ggcggccggg gctgctctgc tgcgcgctcg
    20941 acaccgagct gctcggccac tggtggtacg agggcctgcg ctggctcgcg ttcgtgctcg
    21001 acgaggcgcg gctgcagggc gttccgctcg gcacgctgcc ggacgccctg gaccgcgtcg
    21061 aggccgtcga gcgcccgcta gccgcggcga gctggggcaa gccgaaggac ctcaccacgt
    21121 gggactcccc gcgggtggcg gacatcgcgt tcgccgcgcg gcgcgccgag ctcgacacgg
    21181 tcgccgccgc cgccgccgcc accgcgcgcg accgcggccc cgccctccag cgggcggtgc
    21241 gcgagctgct cgcgctccag gcgagcgact gggccttcca ggtcacccac gacctcgccg
    21301 ccgactaccc gcgcaagcgt gtcgaatccc acgcacgcga gctggacgcc gcgctcgccg
    21361 ctctgaaaga ctccggcgcg ccacccgacc ccgctgtgcg caacctcgca ccggacgtcg
    21421 acctggctcc gctcttcgcc acctgatgcc gctcatcccc aagtgacgcg cgtactgatc
    21481 ctctcgtggg agtacccacc gctgatcgag ggcgggctgg cccggcacgt gcgcaagctg
    21541 gccgagaacc tcgtcgggct cggggtggag gtgcacgtgc tcacgcgcgg ccacgaggag
    21601 tcgcccgccg aggaggagct gcgcggcgtg atggtgcacc gcgtgcgcga gcccgagcgc
    21661 ccgcgcgagc tcgccgagtt cgtcacctgg gtcgagcaca tgaacgcgga catgctcgcc
    21721 gccggggtcg agctgggcga ccgctacgac ttcgacgtgg tgcacgggca cgactggctc
    21781 gtggccgtcg cgggcgatca cctcgcacgg cgcttccgct gcccgctcgc cgtcacgatc
    21841 cacgccaccg agtacggccg ccaccagggc tgggtcgaca agcacccgca gtcgcacatc
    21901 cacggcgtcg agcgctggat ggccaaccgc gccgaccggg tgatcacctg ctcggcgtac
    21961 atgcgcgagc acgtggccga catctacggc ctcgaggagg accggatcgc ggtgatcccc
    22021 aacgggatcg accccagcga cctcgtcacc gtcgaggacc tcgacgcgct gcgcgcccgc
    22081 ttcgcggcgc cggacgagcg cctggtgctg ctggtcggcc ggctcgtgta cgagaagggc
    22141 ttccagctcg cgctcgaggc gctgccgggg ctggtcgagc gcgtcggcaa cgtgcgcttc
    22201 ctggtggccg gctcgggcac gcacgaggac gagctgcgcg agcaggcgac agagctcggc
    22261 ctcgacgagc acggcacgtt cctcggctgg atcggcgacg acgtgctcca ctcgctctac
    22321 cggatcgccg acctgacggt ggtcccgtcg atctacgagc cgttcggcct ggtcgcgctt
    22381 gaggcgatgg cctcgggctg cccctgcctc gtggccgaca ccggcgggct gcgcgaggtg
    22441 gtgccgaacg acgaggtcgg gctgcgcttc cgctcgcgtg acccggagtc gctcgggcgc
    22501 atggccgagc gcctgctcac cgacgacgag ctgcgcgacc ggctgatcgc cgaggcctca
    22561 gagcacgtcc tcaccttcga ttgggccgac gtggccgagc aggcgctgac ggtctacgcc
    22621 gaactggcgc gcgatcgctc gcccgccacc tagccgcctt cataggatcg gcgagcgatg
    22681 agagcgctcg cgtcgttcgt caccggtcgc cgcaccaagc gggtggtgcc ggccatctgg
    22741 ctcctgctcc tgctggtctt cacgccgctg ggcgcgcagc tcacggacgc cgtggacgac
    22801 cgcttcgaga gcttcctccc cgacgacgcc cagtccaccg aggtcgccga gctccagcgc
    22861 gagcgcttcc gctccggcca gacggccgac ggcctcgtcg tctatcgccg cgagggcggc
    22921 ctcacggcgg cggaccggcg caagatcctg gccgacgccc aggccgcgca gcgcgatccc
    22981 gacctgcacc tgaagcgccc gccggtcgcg gcggggcagc cgggcgcgcc ggccgacctg
    23041 ttcgcgccgg atggcgacgc ggcgctcgcc agctacacgt tcgagaacga cttcgagacg
    23101 ctcggcgacg agggcgaggc gctgcgcgag atcacggggg acgggggcgg cggcctggag
    23161 gtctacgtca cgggccaggc cggcttcggg gcggacttca aggaggtgtt cggggagctc
    23221 gacaccaagc tcctgctcgc gacggtcctg ctcgttctgg tgctgctcgg cgccatctac
    23281 cgctcgccgc tgatcgcgat atcgccgctg atcgtcgtcg ccttcgccta catggtcgcc
    23341 caggggctga tctacctcta cacgcagacc ggcgcggtgg tgaacaacaa cgccacgcag
    23401 atcctcgtgg tgctgatgtt cggggtgggg accgactact gcctgctgct cgtgtcgcgc
    23461 taccgcgagg agctgcggcg ggtggaggac aagcacgagg cgatggcgcg cgcgatgcgg
    23521 cggaccggcc cggtgatcgt cgcgagcggc atgaccgtgt cgctgacgat gctgacgctg
    23581 ctcgtggcgg acacgggctc gatcaagtcg ctcggccccg tggcggcgat cggcgtgatg
    23641 tgcgtgctgg cggcctcgct cacgctgctc ccggccctgc tcacgatgtt cgggcgtccc
    23701 ggcttctggc cgcggcgcgc ctcggtcgag ttctcgcccg acgacgaggt gcacgagcgc
    23761 agcggcgtct ggcggcgcgt gggcggccgc gtgctgcgcc gtccggggct ggcgctgatc
    23821 gtcacgctcg cgatcttcgg cctgggatcg ctcgggctat tcgcctacgc cgaggactac
    23881 agcaccgaga acggcttcaa gcagaagacc gagagcgtgg agggcttcaa ggcgctgtcg
    23941 gagtccttcc cctccggcgc gaccgacccc tcgaccgtga tggtggtccg cgagcacggg
    24001 tccgtgaacg atcgcgagct ggccgcggtg gcggcgcgcg tgcagagcga cggcgccgtg
    24061 gccgccgcgg cccccgtgcc ggagcgctcg accgacggcc ggatcggcca ggtcagcgtc
    24121 gtgttccgcg aggatcccta cacgcccgcg gcgttcgacg ccgtcccgcg catccgcgac
    24181 gaggtggccg acgcggtgcc gggcgcgcgc acgatcgtcg gcggcggcgc ggcggtccag
    24241 tacgacttcg acgcctcgac cgagcgcgac ctgaagtgga tcgtcccgct cgggctgatc
    24301 gtgatcggcc tgatcctggc gctgctgctg agggcggcgg tcgcgccgct cgttctgatc
    24361 gcgagcgtga tggcgtcgtt cttcggcacg ctcgggatct cgctgctgtt cttccggcac
    24421 gtggtcggcg acccgggggt ggacgcctcg ctgccggtct tcgcgttcat cttcctggtg
    24481 gggctcggga tcgactacac gatcttcctg atgggacggg tgcgcgagga ggcgcggatc
    24541 cacggcacgc gcgaggggac gctgcgcgcg ctggccgcga ccgggccggt catcaccagc
    24601 gcggggatga tcctcgccgg gaccttctcg gtgctgatca cgatgccgat cacgttcatg
    24661 ttcaacatcg gcttcatggt cgcgctgggg atcctgctcg acacgttcgt cgtgaggacg
    24721 gtgatggtcc cggcggcggt cgaggtgctc ggcgaccgga tctggtggcc gtcggacccc
    24781 tcgggcggcg gcaaggcgct gagcgaggac aggccgggcc cgcccgagcc ggctactccc
    24841 agcgcagctc cctcggcgtc tcccgcatcc tgatcagccg ggcggggacg ccgcccacta
    24901 tcgcgttggc gggcacgtcc ctcgtgacga cggagttcgt cccgatgatc gcgttgtcac
    24961 cgaccgtgac cccgcgcagg atgcacgccc cgtacccgat ccagacgttg ttgccgaccc
    25021 gcacgtcgcg cttgtagatc ccctggaggc ggatcgggcg ctcgacctcc accacgccgt
    25081 ggtcgaagtc gatcagcatc acgcggtcgg cgatcacgca ctcgcggccg atcgacacgt
    25141 gctggtaggc cgagatcgtg cactcctggc cgagcacggt tttggccccg atcgagacga
    25201 ccccctcgtg gcagcggatc ttcgtgccgt gcccgagcca cgaccagcgg ccgagctcga
    25261 tgcgtcccct gcggccgatc tggagcacca cgcccgggcc gatgaacgcg agcccgtcgc
    25321 tgtgcaggcg cctgttcgtg agccgccgcc ggagcagccg gacggccagg cgcgcgtact
    25381 tcgcgttcag catccggtga cggcgcatga agcgcatcag cgtcagcggg ccgccgtgca
    25441 gcgggggagg ggggcggcgg agcaccgcgc gatcgtagga tctccggctc gatgacccca
    25501 cttggcacca ccgccgccga gctgaccgga cgcctgctcg ccggcgacaa gcgcgcgcta
    25561 gcccgcgcga tctcgctggt ggagaacgac gaccccgagg gctgggcgct cgttcgcgag
    25621 gtctacccga agaccggccg cgcgacgatg gtcggcttca ccgggccgcc cggcgtcggc
    25681 aagtccaccc tgatcggcgc gatcgtgaag aacgcgcgcg agcgcgagcg ccaggtggcg
    25741 gtcctgtcga tcgacccgtc ctcgccgttc acccacggcg cgctgctcgg cgaccggatc
    25801 cggctggccg accacttcct cgaccccggc gtgttcatcc gctcgatggc gagccgcggg
    25861 tcgctgggcg gcctatcgga ggcgacgctc caggcggcgc tcctgatgga cgcctcgggc
    25921 aaggacgacg tgctgctgga gacggtcggt gtgggtcagg ccgaggtgga catcatcgac
    25981 cacgccgaca cggtggtgct cgtgctgatg ccgggctccg gcgactcgat ccaggccctg
    26041 aaggcggggg tgatggagat ccccgacgtg atcgtggtca acaagtccga ccacccgctc
    26101 accgacacga tgatccgcga gatccgcggt gtgctctcgc tcggcccgag cggatcgtgg
    26161 aaggtgccga tcctgcggac cgaggctgcg aagggcgagg gcgtgagcga gctggtcgag
    26221 aagatccagg agcatcgtga gcacatcgag gccgagggga cgctccagga gcgccgccgc
    26281 cggaacctga tgaacgaagt gctcgagctc gcgacggtcc gcatgcgccg caagctcgac
    26341 gcgaccgccc gcgagaacga cgaggtgcac gcgctgctcg agcgcgtggt gagccgcgag
    26401 ctcgatcccg cctcggcggc ggcgcagctg ctcgagcggc tgacctagcg gcgcccggct
    26461 cggcgagcgc cggcccctac gggccgcccc cggcgagcgc cctccagcgc gagccgctca
    26521 gggccagcgc gagcgccgcg agcgcgccgg cgccgagcac cgcggccacg acgaacgggg
    26581 cgaccgatcc ctccccgaac agcggcagca gctccgtgag cgggccgaag ggcgcgtcgg
    26641 gccggtcgtc gatccaggtg ccgccgcccc agcgcaggtc cgcgtagagc cagcccgagc
    26701 cgaccagggt gacggctatc aggctcgcgc cgacccgcgg caggcgccgc agcccgagcg
    26761 ccacgagcgg cacgcccagc ggcagcgccg cgatcaggtg ccggcccggg aactcgaagc
    26821 cgaacatggt gggcgcgagg aaggcggcgg tgaggaactg cgctcccacg gcaagcgcgc
    26881 agatcgtgac ggcgcgctcc ccgcggcgca gggccgggat ggcgcgggcc agccggccct
    26941 ggcgctcgcg cagcaaaagc cacgcgccgg cgagcgcgag cgcgagcacg ggcgcccagc
    27001 gcagcaggcc cgcctcgcgg tcgatcagca gggcggcgag gcggtaggcg cggtcgaggt
    27061 agccgccggg gaacgaggcg ttcgtgccgg tggcctcgtc gcccaggccg gcggactgcg
    27121 gggtcggccc cccgtagagg ttgtcgttca ccgacaggta gatcgcgatg ctcaccgcca
    27181 ccacctcgag ccccacgagc gtgacccacg ggcgctgcgc gttgcgcagc gtgcggaacg
    27241 cgatcgcgcc caccacgagc ccggcgggca cgaagctcat ccccagccac gggagcgtgg
    27301 ccaggagcag gaagcaggcg aacgacgagc ggcggctcgg gtgctcgtcg aggcgcaggg
    27361 cgagcagcgt ggcaccggcc agcgcgaccc cggccgcgag cggcggctgg accgccgtgg
    27421 agtaggcgag catcggcggc gacgccgcca cggccagcgt cgcgccgagc gcccacgggt
    27481 ccggcaccgc ccgcagggcg agcaggtagg ccagcaccac cgcgagcgcg gcgatcgccg
    27541 cgaggaagag ctcgacggcg gtctcctcgc cggccgccca ggcgggtgcg atcaggagcg
    27601 ggaagccgac cccgtgcggc tcgttcaggt tgcccgccgt gagcaccccg cgcgggcgca
    27661 tctcgtacgg gtagtactcg ctgtaggccc gcgagcggta ctcgtcgagc aggtcgggcg
    27721 tgcggtcgtc gacgatcgac tgggcggtga gcaggtggtg gggctcgtcg ccgccgtact
    27781 cggaggcgcc gaacgcgtgg atcccgatcg tgctcgcgta gagcccgaag gcgacgatcc
    27841 agaggaggag cgcgcgcctc ctcatgggcg ccgcgccgcg cccgcccgcg gcttcggccc
    27901 gcggcgcacc ggctccgccc gcgccgctag gcgccgagcg cccgcgcgat caccatctgc
    27961 tgcacctcgt cggtgccctc gccgatggtc aggatcttcg cgtcgcggta gtgccggcag
    28021 accgggtact cctcgatgta gccgtagccg ccgtggatct gcacggcctc ctcggtcgcg
    28081 cgcacggcca ggcggcccgt cttgagcttc gccttcgccg ccacaagggt gaacgaccgg
    28141 ccctggtcct tgagccaggc ggcctcgtag gtgatcagcc gcgcggcctc gatctcggtg
    28201 gcgagatcgg cgagcttgcc ctggatcgcc tggaacttcg agatcggctg gccgaacgcg
    28261 cgccgctcct tcgagtaggc gagcgcctcg tcgagcgccc cctgcgcgag gcccacgccc
    28321 atcgcggcca cgccgatgcg gccgccgtcg agagtctgga ggaactgctt gaagccctgg
    28381 ccgcgcgggc cgagcaggtt ctcctcgggc acccgcgcgc cgtcgaagct gagcggccgc
    28441 gtgtcggacg cgttccagcc catcttgcgg tacggggtgc cctgctcgta tcccggcgtc
    28501 cccttgggca cgaggatgtt cgagatctcg ttctcgccgg tcttcgctgt gatggccacg
    28561 tggccggaga tgtcggtgcc cgcgttggtg atgaactgct tggcaccgtc gatcacccac
    28621 tcgccgtcct cgagcgcggc gcgggtcttc acgttgccgg cgtccgaccc ggcctccggc
    28681 tcggtcaggc cgaacgcgcc gagcttgcgg ccggcgcaga ggtcagggag cagttcccgc
    28741 ttctgctcct cgctgccgaa ggcgtagacc ggccaggttc cgagcgaggt gtgcgcggcc
    28801 atcgtgatgg ccacgctcga gtccacccgc gtgagctcct cgacggcgat cgcgtacgag
    28861 aggttgtcgg tcccgccgcc gccgtactcc tgggggaacg gcatccccat gaggccgagc
    28921 tcgccgagtt gggccacgat ctcgtagggg aaccgcttct cgcggtcgag ctcctcggcc
    28981 acgggacgca cctcgttctg ggcgaagtcc cggaccagct cgcggatgtt gcgctgctcg
    29041 tcggtgaggt cgaagttcac gggagcggcg cagaatagtc acgtgcccgg cacatctgtc
    29101 acccacgtgt aacgcggcgc cgcttacggt cggccccatg cggtccgagc gtggacaggc
    29161 gagcgtggag tgggtgggcg cggtgctgct cgtggcgctg gtgctcggcg gcgccacggc
    29221 cgcggcgggc gacgtggacg gccgctccta cggcggctgg ctcgcgcact cgatcgtgtg
    29281 cgcggtgagc cgcggctgcg ccgagcaggc gggggagctg gccgcggcgt acggggacag
    29341 tgacgccgag ctggtgcgcc gctacgcgcc caacctcgtc tacgagccgg gcacctacac
    29401 gctgccggtc gacttccgcc agtgccgctc gcaccgctgc gccgatgcgc ccgacgaccc
    29461 cgacctcgac gcccacctca ccacacgcgg tggatatccc gccaccgcgt tcacgcacat
    29521 cgtgcaccgc gacggcgaga cgttcatcca gtactggctc tactaccccg actcgaacag
    29581 cacgttcgcg ggctcgtcgc gagcctggaa cgcggtgcgc cgggtcgagc cgtggatggg
    29641 ggactacccg ggctaccacc gcgacgactg ggagggggtc caggtgcgga tcggccccga
    29701 cggccgcgcg cgaacccgag ccagcgccca ccacggctac cagtggtgca agcagcagcg
    29761 ctgcaagaac agctggggcc cctggaccgg ctggtcgcgc gtatcgcgcg gcagccacgc
    29821 cggccatgtc ccgacgcgct cggatccgct gagcacgcgc atccgcccgg cccaccccgg
    29881 cgccgacacc cacgagcgca ccacgacgag cggcggactg cgcctgatcc cgatcgagac
    29941 gctggacacc ggcgcctaca agccgctcga ccccggcatc acgccgccgt ggcggaagag
    30001 ggtctacacc gacccgctga gcgactcgac gtcgtgagcg ccgctagcgc ggctggcccg
    30061 tcggccgtac caggatctcg ttcaccgaga cgtgctccgg ctgcgagatg gcgtacgcga
    30121 tcgcgttggc gatgtcgccg ggctcgagga ccttcccgat ctgctcgcgc atcttgccga
    30181 tcgtctcgac caccacaggg atgtcgttgt ggccctggag ctcggtgtcc acgaaccccg
    30241 gctcgacgat cgtcacccgg atcttcgccc agccggtctc ctggcgcagc gcctcggaga
    30301 aggcgccaac gccccacttc gtcaggttgt agaccccgct gccggcggac gcaacgcggc
    30361 ccgccaccga cgagacgttc acgatgtccc caccgccgcc ctcgcggagg atcggcagcg
    30421 ccgcgtgcgt gcagtacagg agccccagca ggttcacgtc caccatccgg cgccagtgct
    30481 cggtgtcggc cccgtcgatc gggccgagca gcatcacgcc cgcgttgttc acgagcccgt
    30541 cgagccctcc gagcttctcc gcggcgccct ccacgaacga ccgcgcgctc gcctcgtcgc
    30601 ccacgtccac cggaacggcg tgcgcggtcc cgcccgcacc cgagatccgc tcgaccagct
    30661 ccccgagccg ctcctcgcgc cgggcgccca cggccaccgc cgccccctgc gcagccagca
    30721 gctccgccgt cgcggctccg atgcccgacg acgcgccggt gatcgcgacc ttccggccgt
    30781 cgagcgcgcc ggccatcagc tccgccccgc ctgctcggcc agctcgacca gcatccgcac
    30841 gccgaacccg gaggcgccct tcccgacgta cgagcgcccg agcccccagg ccgtgccggc
    30901 gatgtccacg tgcacccacg gcgtctcgcc cacgaactgg cgcaggaact cggcggcgta
    30961 gatcgacatc gccttgcgct cctcggccgc gttcgtgagg tcgccgtact tacccttggt
    31021 cagctcgagg tactccgggt gcagcggcag ccgccagccg agctcgccgg tggcgttgca
    31081 cgcggcctcg acctcgccgc accagtcgtc gtcgttcgag aagagccccg cgtaggtgct
    31141 cccgagcgcg acgatgatcg cgccggtgag cgtcgcgagg ttgatcacgc gctcggcgcc
    31201 ctggtcgacc gcgtaggcca gcgcgtcggc cagcagcagc cgcccctcgg cgtcggtgtt
    31261 gttcacctcg atcgtcttgc cgttcatcgc ggtgacgatg tcgcccggct tgaccgaccg
    31321 cccgctcggc atgttctcgg tcgaggggac gacggcgagg atccgcgcgg gcacgcccag
    31381 ctcggctatc gcgcccatcg cctcgatcac ggcggcgccg ccggacatgt cgaacttcat
    31441 ctcgtgcatg cgcccggccg gcttgatcga gatgccgccg gtgtcgaagg tcacggcctt
    31501 gcccacgtaa ccgagcagcg gccccgagcc gccgccctcg tagcgcatga cgatcatgcg
    31561 cggctcctcg taggtgccct gggccacgga cgcgaacgcg cccatcccgg cctcgacgat
    31621 ctgcgcgcgg tcccagacct cgatcgtcag cgagtcgtgc gccgcggcga tctcggacgc
    31681 ccgctcggcc agccaggtcg gcgtggccac gttggcgggg tagttctgga ggtcgcgcgc
    31741 ggcgttctgc gccacgccgc gaacgcggcc ggactcgatc gcctgcggat cgaccccctc
    31801 ggccgacgcg atctcgagcg agccgatggc cgcgtcgtca ccgtcctcct tcgtcgactt
    31861 gaactggtcg aagcgataca gggcgagcag cgtgccctca acgagcgcgc cggccacctc
    31921 gcccccggtg ggcgcggccc acgacagcga gcgcgccccc agctcccgcg cgcgcccggc
    31981 ggccacggcc gcggccaccc gcgcgcgctc gtgctcgagc tcctcgcgct tgccgagacc
    32041 caccagcacc acgcgccggc cctcctcgtg cgtgaccgcg agcttgcgca gccccgcctt
    32101 ggcctcgccc gagtcgacca gcgcctgaag ggccggatcg tcgagcgtct cgccctcgaa
    32161 gaccggcacc acacgggtgt cggcatcggt ttcgcggggg tctcccgcac gtcctgtaac
    32221 tgtgatgttg gccacggccg gggcagtcta cgcaccgcta cccttcgcca cccaccgacc
    32281 caggagatcg agcagaccga tgcccaagac agtgatcctc gggaccgccc gcacaccgtt
    32341 cggaaagctc ggcggagccc tgtcctcgct cgacgcgacc gacctcggcg gtcacgccat
    32401 cgccgcggcc ctcgagcgcg ccgaggtggc cccggagcag gtcgagcacg tgtccttcgg
    32461 gcaggtcctc caggccggcc aggggcagat cccctcgcgc caggcgcaga tcaagggcgg
    32521 catccccaag gaggtctcgt cggagaccgt caacaaggtc tgcgcgtcga gcatccgcag
    32581 cgtcacgatc gtcgacagcg ccgtgcgcgc cggtgacctc gacgtcgccg tcgccggcgg
    32641 catggagtcg atgtcgcagg ccccgtacct cctcaagcag gcccgcttcg gctaccgcat
    32701 gggcgacgcc aaggcgctcg acgcgatgat caacgacggg ctcacgaatc ccttcacgaa
    32761 gaagcacatg gcccaggagg ccagcgaggt cggcgacgag ctcgagatga cgcgcccgga
    32821 cctcgacaag ttcgcgctgc gcagccacga gctcgcgatc caggccaccg acgagggccg
    32881 catccccgag gagatcgcgc cggtcacggt caaggcgaag aagaaggaga ccgtggtgga
    32941 ggtcgacgag gcgccgcgcc gcgacacctc gctcgagacc ctcgccaagc tgccgccgat
    33001 cttcgtcgag gacggcaccc acaccgccgg caacgccccg ggcgtcaacg acggcgcggg
    33061 cgcgctcgtg ctcgcctccg aggagtgggc caagcagaac ggccgccagc cgctggccac
    33121 gatcgtcacc catgctcagg tcgccgacga cttcccgtac ctcgcccgca cgccggcgaa
    33181 cgccaccaag aaggcgctcg agcaggccgg cctgacggtc gacgacatcg acctgttcga
    33241 gatcaacgag gcgttcgcct cggtcgccat caacagcatg cggatgctcg aggtggacga
    33301 gagcaaggtc aacgtgaacg gcggcgcgat cgcgctcggc cacccgatcg gcgccagcgg
    33361 cgcgcgcatc ctcggagcgc tcgtgcacga gctgcgccgg cgcggcggcg gccgcggggt
    33421 cgcggccatc tgctcgggtg gaggccaggg cgacgccgtg atcatcgagg taaacggggg
    33481 ctaacgcgcc acccctcctc cgggtggggg agccccgacc ggagggtttt gctcaacccc
    33541 cgtttagcgc tgtcgcggca gaggtacagc cgtcccccag gccaaacgaa tcagaggggc
    33601 ggaagcatga ggggaaggca ttgcgcggtc gtctgcgcgc tggtagcggc agccgtttcg
    33661 agcacggcgg gaccggcgtc ggcacagata tttccggggg actgcttcgt cagcgacggc
    33721 ggacgcatcc tgaccgcaac gggcagcccg gccacgttcg gcggcagcgc gtcgagcacg
    33781 agcggcgcgc aactcggcca ccaggtctac atcgacgacg gccctctgac gggcttcagg
    33841 ttccgttcga tcgcgatgac cgcgatgctc tgcaatctcg acgcccatcg cgccgagatg
    33901 tacggcagcg gcgaggtgac cacccagctc ggcctgagcc agcccgtgga gtaccggatc
    33961 gccgtgctcg gcgtgcgcag cacgtcgcag gtgcccgaca cctaccggat cacgctgtcg
    34021 aacggctacg actccggcga gcagccggtg atcgacggca acatcgccgt cagaggtacg
    34081 taactcgacc tgcatagcct tggcgccatg caacgtggcg ccgccttctt cgacctcgac
    34141 aagaccctca tcgagggatc gagcgccgtg cacttcgcgc gctcctcata ccgcgcgggg
    34201 gtgatgaccc gtgggcagct cgcgcgcgac ctctgggcga acgtcaagtt ccgcctcgag
    34261 gggagcaccg acgacatggc cgacatgctc cgctcgcgcg tgctggacgc gatcgcgggc
    34321 cggcgcgtgg tggacctcgc gcggctcgcg ccgggcgccc tcgcgggcat cctgccgcgc
    34381 gtctacccgg agatgctgaa ggaggcgtgg gcccatcagg acgccgggcg gccggtgtac
    34441 atcgtgacgg ccgcctccca ggagatggcc gagatgatgg cccacgtgct gatcttcgac
    34501 ggcggcgtgg gcatgcgctc ggagatcgcc gacggcgtct acaccgggcg ccccgacggc
    34561 ccgttcacct accgcgaggg caaggccgtg gcgatacggg agatggccga gcgcgagggg
    34621 atcgacctcg cggagtcgtt cgcgtactcg gactcggagt ccgaccttcc gatgctgcgc
    34681 ctcgtcggca acccggtggc cgtcaacccg gacgcgcacc tcagccgcgt cgcgcgcgag
    34741 gagggctggc ggatcatcac gttcgatcgg ctgcaccgcc gcgtgcggat cgcgaccacg
    34801 gccgccgcgc tcgcgggagt gggcctcgcc gggggctggg tggcgggccg agcccgtaca
    34861 ctctcggccg ggtggggaag gcgtcgtaca cgcttgccgt tgcgctgatc gcgagatcga
    34921 gcgctccgcg cgcgctggcc gcgctggcgg ccgtgatcgc attcgcagcc gcgcccgccg
    34981 gtgcgcagac acccgccccg cacccgctca cgggcaaggg gcagaacttc gagatcgtgg
    35041 ccaacgtgcc catggacggc ggcagtggcg aggtcgcggc ctctgacctc gagctgcacg
    35101 gcgactacgc gttcgtcggc tcctacggcg agggcatggt cgtggtggac atctcgaacc
    35161 cgttcgcgcc gaagcgcgtc ggcaagttcg actgcccggg cggccagaac gacatccagc
    35221 tctcgcccga cggcaagacc gtggcgatgg cgatcgacac gaccgccaac gagtgccacc
    35281 cgaacgacga gggcacctcg atcatcgacg tctccgaccc ggcgcagccg aaggagatcg
    35341 cctggatcga caagaaggag ctgccgcgcg ggtcgcacaa caacacgctc gactggccct
    35401 acctctacgt ggaccagtac tcgacggcgt actcgcagct cgagatcttc gacctctcga
    35461 cgccgtcggt gccgcgcaag gtcggcgagc tgagctacgg cggcgaggac tcggtgcacg
    35521 acctctcggt cgaccaccgc ccggacggca ggtcgttcgc gtacgcggcc tcgatcggct
    35581 tcaccgatgt gatcgacgtg accgacccgc gcagcccgat cctgcgcgag cgcgtgcagg
    35641 acccgcaggt gacgatctcg caccaggccg aggcgaactt cgaccgcacg ctgctgctcg
    35701 tcaccgacga gttcaacggc ggcgcctcct acagcggggc ctgcggcgga tcgccgatca
    35761 cgcgcgtgcc gttcccgacc ggcgccccgg ccgtgggcga cccgcagaac atcggcgcgc
    35821 tgcacatcta ccggctcgac ccgagcggca acatcgccgg ctccgggggg ctcgacaagg
    35881 ccggcacctt caacatcccc taccaggcga acgacgagcc ctcggccggc tgcacgatcc
    35941 acgtgttctg gcaggcgccg gacgagaacc gcatggtcac ggcctggtac gggcgcggca
    36001 cgcgggcgat cgactactcg aacccggccg ctccgaagga gctcggctac ttcatcccca
    36061 ccggctccga cacctgggcc gccaaaccgc accgcggctt catctacaca ggcgacatcc
    36121 tccgcggcat ggacgtgttg cgctacaagg gcgagggcgg cgcccgctgg ccagccacgt
    36181 cgggcccggc cgagctccag cgcgcgcgcc agcagggcgc ctctccgccg gtgccgaccg
    36241 gcgcgccggc ccccgcgcag cccgcgcccc agcagggcga gcagccccag ccaacgcagg
    36301 ggccgcggct gcgcgtcggc cgccgcgtct tcaccaagca cgtgcgcgtg ccggacgtgc
    36361 cccgcaagcg caaggccgag ctggtgctct cgttctacaa gaacaagggg ctcgtcacgc
    36421 ggttgcggct gcgcacgcgc gaccggcgga tcgcgaagct gcggacccag gccgccggcc
    36481 tcgcgggcca ctaccgctac acgatcaggc tgggcgaccg gggcaggttc ctggcccgcg
    36541 ggatcgtgcg cgtgcgccgc tcggacacca cgcgagtcga actgccagcg gggcaggcgc
    36601 tgatctgccg cgtgtcggcg aagtagcctt tccccatggc caccgcccca agggcgatga
    36661 cggacgccga gcgctggtac cgcgagcgct actcggccag tcctgagcgc gacgcgctgt
    36721 tcaccacgct ctcgggcgag gaggtcgagc cgctctacac cgccgaccac ctcggcgacc
    36781 ccgacggcat cggcctcccc ggcgagttcc cctacacgcg cggcgtctac ccgtcgatgt
    36841 accgcggccg cctttggacg atgcgccagt tcgccggctt cggcaccgcc gaggagacca
    36901 acgagcgctt ccgctacctg ctcgaccgcg gccagaccgg gctctcgacg gcgttcgaca
    36961 tgccgagcct catgggccac gactccgacc acgcgcgctc cgagggcgag gtcggccgcg
    37021 agggcgtcgc gatcgacacg ctcgacgaca tggaaacgct gttctccggg atcccgatgg
    37081 ccgaggtctc cacctcgatg acgatcaacg ccccggccgc ggtgatgctc gcgttctacg
    37141 tggtcgccgc ggagaagcag ggcaccccgc ccgagaagct cgcgggcacg atccagaccg
    37201 acatcctcaa ggagtacatc gcccagaagg agtggtgctt cccgatcgag ccggccatgc
    37261 ggctcgtggg cgacatgatc gagttctgct cgcggcggat gccgcgctgg cacccggtct
    37321 cgatctccgg ctaccacatc cgcgaggccg gatcgacggc tcagcaggag ctcgcgttca
    37381 cgctgaagga cggcttcacc tacgtcgagc aggcgatcga gcgcggcctc catcccgacg
    37441 agttcgcgcc gcggctgtcg ttcttcttca acgcccacat cgacttcttc gaggagatcg
    37501 ccaagtaccg cgccgcgcgc cggatctggg cgcgcgagct gcgcgacacc tacggcgcgc
    37561 aggacccgaa gtcgctcctg atgcgcttcc actcgcagac ggccggcgtg tcgctcaccg
    37621 cgcagcagcc gctggtgaac atcgtccgca ccgcaaccga ggcgctctcg gccgtgctcg
    37681 gcggcaccca gtcgctccac acgaactcct acgacgaggc cctggcgctc ccgaccgagg
    37741 acgccgtgag catcgcgctg cgcacgcagc aggtgatcgc ccacgagacc ggcgtgacca
    37801 acacgatcga cccgctcggc ggctcctact tcgtcgaggc tatgaccgac cggatggagc
    37861 gggcggccta cgactacttc gcgaagatcg acgagctggg cgggatggtc gaggccatca
    37921 agcagagcta cccgcagcgc gagatcgccg acgcgtcctt ccgctaccag caggaggtcg
    37981 aggacgggca acgcatcgtg gtcggggtga accgctacca ggatcccaac gaggatccgc
    38041 tggccatcct gaagatcgac ccgggcctcg agcgcaagca gatcgaccgc ctggcgggcg
    38101 ttcgcgcgcg ccggcgcggg gaggaggtcg agacgtcgct ggcccggctg cgcgaggccg
    38161 cggcgaccga cggcaacctc atggagccgc tgatcgagtg cgcccgtgcg cacgcaagcg
    38221 agggcgagat agtcgagtcg ctccaggcgg tgttcggcag ctacaccgag acccccgtct
    38281 tctagtgcgg cggcccgtgg agatcgacct ccctgagggc gccgtcccgg accccgcgac
    38341 cgtcaccccc gcgggcgagc ggctggagcg cctgatcgac ggcgtaatca cgcgccgggt
    38401 ggtggtccag agcgacgagc gcggcaccgt cagggagatc ttcaacccgg cgtggggctt
    38461 cacggacgag ccgatcgtct acgtctacca ggtgacgatc tacccggggc agaagaaggg
    38521 ctgggtggtc caccgcgagc aggacgaccg gctcttcttc gatcagggcg ccgcgaagct
    38581 cgtcctctac gacgcgcgag cggactcacc gacgaagggc atgatcaacg agctgttcgc
    38641 cggcacgaac aaccggctgc tcatccggat cccggcgggg gtcgtgcacg ccgtcgtcga
    38701 cgtcggcacg gacgaggtga gattcgtgaa cctcccgacg cgcccgtaca accacgagcg
    38761 gcccgacaag atgcggctgc ccgacgacgc cgtgccgtac cagctctgag gctcgaccac
    38821 gtcgcaggcc tggtgctcgg gatgctcgcg gacgagtacg tccgcccgcg gccccgcgct
    38881 tcggacgggc cgctcgtcac cgtggtcatc ccgacctaca accgcagcga ggtgctgcgg
    38941 cacgcgctgc ggagcgtgct cgaccagagc taccgccggc tcgaggtgat cgtcgccggt
    39001 gacgcctgca ccgacgactc cgaggaggtc gtcgcctcgt tcgccgaccc gcgggtgaca
    39061 tgggtcaacc tcgaggtgaa caccgggagc cagggcggcc cgaacagggc ggcgctcgag
    39121 atcgcgaacg gcgagctggt ggcgtacctg gggcacgacg acctctggcg ccgcgatcac
    39181 gtcgcgctcc tggtggcgga catccagagg agcggcgccg atgtcaccta cggcgcgtgc
    39241 gactccgtgt tctcgcaggg tcgcatcgcc ggccggcgct tcacctgccc gcccctcggc
    39301 gagccggcgc tgccgtcgag cgtgatgcac cgccggtcga tgtgcgagcg cggcgccgag
    39361 tggcgcgact ggcgcgagac ggtcaacgca cccgactacg cgttcttcga gagcctcgtc
    39421 gccgcgggcg cgaagctgtc gcgcgtgccg gccctgacgg cggtgaagtt cccggcctcc
    39481 gcgcgccgca acgtctaccg cgagcaccgc agccgcgagc aggagcgctt cgcgaggagg
    39541 atgaacggtc gcgcgttcgt cgcctgggag atcctcacgg gcctcctcct gctcccgctg
    39601 cgccgccgca cgcgcctccc gatcgaccag cgggcgtaca ccgagcccgg cgagctcgtc
    39661 accgcgctgc gacgcgtccg cgggctcgag tgacccccgg gcgctagggg atcgcgaggg
    39721 tgcccacccg gcgcgaatgg cgcgtgccga ggtggagcgt ccagaacccg agcgggagca
    39781 cgatcgcggc gcccgcggcg aggcgcgcga gggccgcagc gtcgatctcg ccgcgcagca
    39841 gcgcgtggcg gacgatgtcg acggagtcgt cgaacggcag cacggtcgcg agcgcgtgca
    39901 ggggctgcgg cagctcgtcg atcgggaagt aggcgccggc cagcagcggt acggcgatcg
    39961 tcgccagcgc gctcagcgac aggccgagcc ggaggaccag gtagaggccg gtgaggccgg
    40021 cggcgaggcc gagcgagacg acggccgcgc cggcgaggcc gatcaggacg ccggccacgg
    40081 cgaccgcgcc gagatgcagg tcaggctcga ccaggcccgc ggccagcagg acgaggaaca
    40141 ggccgagcac gcagccacgc agcagctcga acgccgcctc gccggccatg accaccgcgg
    40201 aggagaggcg cgagctgagc agctgggcga gggagccgag cgccacctcg ccctcgaagc
    40261 ggtggacgat ccgcggcagc gcggcgttga cccgcaggac ggcgagcccg gcgaccacga
    40321 acgggaacag gtcgccccgc ccgggccctc gaccggcgaa tctcgccagg tagtagaaga
    40381 cggcgacccc cgccgcgacg gcgagcaggt cgatgacgta cggcagcctg tgcgtggcct
    40441 ccacgcgcgc gtcgcggcgc gcgaacgcgg cgatcgacgt caacgggtgg gggctcatgc
    40501 ggccatcagc gcggtcacgt cctcgaggcc cggatcgtgc cggagctcgc gcgccacccg
    40561 cccggcccgc agcgcgacca cccggtcgcc gagctggacg gcctcctcgg cgtcgtgggt
    40621 cgcgagcagc acggcgggcg ggcgatcgtc ggttctcagc gagtcccgga actcgcgcac
    40681 cgagcgcgcg tccagcgcgg ccgtgggctc gtcgagcagc aggaccggcg ggcgcgcgag
    40741 ccgggcccgc gcgagcgcga gccgggcccg catccccgtc gagtagccgc ccacccgccg
    40801 gtcggcggcc tcggcgagcc cgaccgcgcc cagcgcggcg tccaccgcgc cggaggccgc
    40861 atcgccgagc gcctccaggc tcgcgaagaa ctcgaggttc gcgcggccgc tcagccgggg
    40921 gtaccagccc cccggcccgc cgaggacggc gccggtccgg gctcgtgcgg cgcgccgctc
    40981 gcggacgacg tcgtgaccgc agacgagcac gtcgcccgcg tccggctcga gcgtcccgga
    41041 gaggagttgc agcagcgtgg tcttgccgga gccgttcggc ccgacgagga cgacggtctc
    41101 gcccggctcg accgcgaggt cgatcgcgtc gagcgcgagc acggcgccga accgcttcgt
    41161 ggcgcctctc aactcgacga ccgccgccat ccgcgggacc gtagccggtc gcctgaacct
    41221 tctcgcgcta ccgtccgtac aactcgccat gcgcagactc gccctccccc tgatggccct
    41281 cgtggctctc gccttcccgg caaccgtcgc ggcggccaag acgtacacgg tcaaggtcgg
    41341 cgacgactac ttcagcccca ccaagcgcac ggtcaaggtg aacgacatcg tgaagtgggt
    41401 ctgggtggcc gaggacggag cccccggctc gaccgtcaac gagcacagca tcgtcgagac
    41461 caaggaccgc ttccactcga agacccagac ggccggcagc tacaagttcc gcttcaagaa
    41521 ggcgggcaag ttcacgatcg tctgcggcga gcacccggag accatgatct tccgggtcac
    41581 cgtcaagaag tagactcgcc cgccggatgg aagccggctc ccagcgaaag atccgcgtcg
    41641 tcgtggccaa gcccggcctc gacgggcacg atcgcggcgc gaaggtgatc gcgcgcgccc
    41701 tgcgcgacgc gggcatggag gtcatctaca cgggcctcca ccagaccccg gagcagatcg
    41761 ccgagaccgt gctccaggag gacgccgacg cggtcggact ctcgatcctc tcgggcgcgc
    41821 acatgacgct cgtgccgaag gtggtccgcc tgctgaacga gcagggcgcc gacgacgtgg
    41881 tggtcacggt cggcggcacg atccccgccg aggacattcc ggagctgaag aagctcggcg
    41941 tggccgagat cttcacgccg ggctcctcga cgcaggagat catcgacttc atccagggcg
    42001 ccgtcggcac ccgcacctag taggcgacgg ctgcaaacct tcgcctgcgg gcgccctcgg
    42061 tcgctcgcgt gctggcgcac gcgtcgcccc ctgcgtcctt cgcatgctcg gttttcgccg
    42121 ccgctccctt tcgagccccg tctcgtacac tttcaccaca ggcgattgac tcgtcagtca
    42181 ataagggcgg cagcggttag aatgccgcgc cgcaccgacc gtaaggagct ttccccttga
    42241 agatttgcgt cctcgtgaaa gaggtcccgg acgcggccgt ggagaagcgc atggaccctg
    42301 gtaccaagcg cctcgaccgc tccggtgaga agaacctgaa ccccttcgac acgcacgcca
    42361 tcgaggcggc gatgcaggtc aaggagggcg gcgtcgcgga cgtcgaggag gtcatcgccg
    42421 tgacgatggg gccggagagc gccgtccgcg cgctccacaa ggccgtgtcg ctcggcgccg
    42481 accgctcggt ccacctgacc gacccggccc tcgaggactc cgacgtcgcc gccaccggct
    42541 acgcgctgtc ccaggtgctc aagcgcgagc agcccgacct ggtcctgctc ggccagcagt
    42601 cggacgacgg cgagtgctac acgatcggcg cggtcgtggc cgaccacctc cagatgccgt
    42661 cgctgaccca ggtcatcaag atggatgtcc aggacggcaa gctgcgctgc gagcgccagg
    42721 ccgagtacgg ctacgacacg gtcgaggtcg acctgcctgc cgtcatctcc gtgggcgacg
    42781 cgatcaacga gccgcgctac ccgtcgctca aggcgatcat gggcgcgaag aagaagccgc
    42841 tcgagagcgt cggcacgggc gatgtcgaga tcgagacgag ccgcgtcggc agcgagggca
    42901 agcgcaccgc cgtactcggc atcaaccccc cgccccagaa ggaagcgggg cagatcatcg
    42961 aggacgagga caccgacgac acggtcgaga agatcgtcgc gtggctcgac gaaaggaagc
    43021 tgctggcatg agcgggatcc tcacctacgc actccactac cagggcgagt tcaacaagaa
    43081 ctcgctcggc gccgtcaccg agggcgcgcg gctggcgaag gagatcggcg gcgatgccgc
    43141 ggtcgtcgtc gtcggcggcg acgacctgag cgacgacctc tgcgcgtcgc tcggcaagta
    43201 cggcgcgacg aaggtcttcc gcgcgaatgg ccccgagggg ctcggcgcac cggtggtcga
    43261 cgtgatggcc aaggtcatcc aggacaacga ctacgactac gcgctgttcg gcggcggcct
    43321 gctcggcttc gagatcggcg ccggcctcgc tgctcgcctc aacggcggcg tgacgatgga
    43381 ggtcacggcg gtcaaggcgc aggacggcaa gctcgtcgcc gagcgcccca tcctccagga
    43441 ctcggccatc gtcgacgtcg agtacgtcgg caagcccggc atcatcatcg gccgcctgaa
    43501 cgcgttcgag atggccgagg ccggcgaggg ctcggcgagc gtcgaggacg tcgacgtcca
    43561 gtactcggag tggtcgaaca aggcccggat gatcgagcgc ggcgagcagc gcggcgccgt
    43621 cgtgaacatc gaggacgcca tcatcctcgt ggccggtggc cgcgggctcg gcacggccga
    43681 gggcttcgag ctgtgcgagc agctcgccga ggcgctcggc ggagcggttg cggccacccg
    43741 cgcggtggtc gacgcgggct ggtacccgta cgcggctcag atcggccaga ccggcaagac
    43801 ggtcgcgccg aagctctacc tcgcggccgg catctcgggc gcgatccagc acaaggtggg
    43861 catgcaggcg tccgagaaca tcgtcgcgat caacaaggac cagaacgcgc cgatcttcga
    43921 gttctccgat ctcgggatcg tcggcgacct gaacaagatc ctccccaagc tcaccgaggc
    43981 cgtgaaggcc aagaggggcg gctagtagcg cagcctgtcc gtcccgccgc tcgcctgacg
    44041 agcgcgccga gcagacacag cccgccgcgg cggccgggct aacttcaccg ccatagcccc
    44101 gcgactgaga caccaacccc gccgcgcgtc ggtggtcggc gccggctcgt tcggcacggc
    44161 cgtcgcgctg ctgctcgagc gcgccgagat ccggaccacg ctgctctgcc gcaccgaaga
    44221 gcaggccgag cggatgaacg aggcgcgcga gaacgagaag tacctccccg gcgtgcagct
    44281 ccccgaccgg ctgaaggtcc gcacgttcgg cgggcgcgac gaccagttcc accgcgcgga
    44341 cctcgttttc gtcgcggtgc cgtcggccgg catcgcggac gccatcgccg agctcgagcg
    44401 ccagggcgtg aacgaccgcg cgggggtcgt ctcgctcgcc aaggggctcg tcccgccgga
    44461 cggcactccc cccaccgagg tgctcgaccg cgccttcggc accgagcgcg tggcctgcgt
    44521 gggcggaccc gcgcacgcga aggagatggt ggagggcggc gcgggactga tctgcgcctc
    44581 gcagccaccc gcgctggccg acaccgtcgc gtccgtgctg cgcgaggcgg gggtggtgtg
    44641 cgaggtctcg acggaccccg tgggcgtgga gctggccggc tgcgccaaga acgccgcggc
    44701 gctcgcggcg ggcgccaccg agccgcaggg gctgaacgcg gccggcatgg cggcggccga
    44761 catcttcggc gaggtgctgg cgcttgcgga ggcgcgcggc gggcgtgcgc ggacgttcct
    44821 cgggcgggcc ggggtgggcg acctcgtggc ccccgcgatg gcgccgaagt cgcgcaaccg
    44881 cagcgccggg gagctgctcg ccgagggcgt gcccgcgcgc gatatccccg atcggctcgg
    44941 ccaggcggtc gaggcgctcg agactgtccc gctgctggcg cgcgcgatcg agcgggcggg
    45001 gatctcggcg ccgatcacct ccgcgctcgc gcgcctgatc gagggccatc tcccgctcga
    45061 cgactgggtc gcgctggtcc gcgtcgcgca gccggatccg gagcgccgcg accgcggcct
    45121 gcgggcctgg ctgcgccgga tccgcaggcg cctcaacggc ctcatgcgca ggtagtcgcc
    45181 ccgactacac tcgaatacat gaaggcggac atacatcccg agtacgtaga ggcccacgtc
    45241 cgctgcactt gcgggaacga gttcacgacc cgctcgacca agccggagct ccacgtcgag
    45301 atctgctcga actgccatcc gttctacacc ggacggcaga agctcgtgga caccggtggt
    45361 cgggtggagc ggttccggcg ccgtcaggcg cgcagcggcc gcagcgcgtc ctgacgcgcg
    45421 cgtcaactta gaccgcaact tcagcggacg gaagctgttc aaaaagcgcc atgcgcatgc
    45481 gcggactcgc actcctgctc acctcggctc tcagcctcgt ggccctctcc gggaccgcgc
    45541 aggccgctgt gcccgcggcc aaggtcgtgg actgccggac cggcaaggca cccgagctga
    45601 ggctggccac gttcgagggc cgcatgacgg cgatcagggg cacgcaacgc atgcagatgc
    45661 gcttcgagct cctcgagcag acccccggca ccgccgagcc ccagcccgtc aaggcgccga
    45721 cgctcacacc gtggcggcgc agccgcgtgg gcgtgaaggc gttcacctac gcgcagaccg
    45781 tcaaggggct cgcgtcgggc gtcacctaca gctcgcgcgt gtacttccgc tggctgagcc
    45841 cggccggcaa ggtcctccgc agcgagcagc gcgactccgg cacctgcgtg caggacggcg
    45901 ccctcccgaa cctggtcctg gggtcagtca agtccacgcc gggccaggtc gaggggacgg
    45961 cggtctacac ggtgcaggtc ggcaacaccg gtcagggcga cgcggagtcc ctgacggtca
    46021 gcctgatcgc cgacggcgcg cacatcgaca cgcggacgat cgacggcctg aaggccggcg
    46081 agttcaccag cgtgaggttc accgggccct actgccgccg cctgcgcgcg atcgtcgatc
    46141 gcggggcgac cgtgcccgag accgtcgaag aggacaacga gttgcgcgcc cgctgctgag
    46201 cacgcgcccg cccgtcctta cgaacccctt tcaaagcgcc gatacgatcc tcgtatgagc
    46261 gggaccgccg agacggcgca ggggatgcag gcccgcaagg acgcgccgat cggcggccag
    46321 gcggtgctcg agggcgtgat gatgcgtggc gtctccgtgt gggccgtcgc cgtgcgcaag
    46381 ctctccgctg agcaggtcgc gcagggcgag gaccgcgaca tgcgcgaggc agccgccggc
    46441 gagatcgagg tggtctccga gccgctcgtc tcgtgggtga agcgccaccg ggcgctcagg
    46501 ctgccgatcg tccgcggcgt cgtcgcgctt gcggagtcgc tgaagatcgg cttccgcgcg
    46561 ctcgggatct ccgccgatgc ccaggccgcc ggtcacgacg aggagatcgg caaggggatg
    46621 tgggtcggca cgatcatctt ctccctggcg ctcgccgttg ggctcttctt cctgctgccg
    46681 gtgggcctca cgagcctctt caaggactcg ctgccgaact cgctcgtctt cgtgatcgtg
    46741 gagaaggtga tccggatctc gatcttcctc ggctacctgt gggcgatatc gaagatgcgc
    46801 gagttgcagc gcgtgttcga gtaccacggc gccgagcaca agacgatctc gaactacgag
    46861 tccgggctgc cgctcacgcc ggagaacgcg cagcagttct cgcgccttca cccgcgctgc
    46921 ggcacgagct tcctgctgat cgtgatgatc gtggcgatag tcgtgttcgc gccgctcggc
    46981 acgccgtcgc tgccgatcct gttcgcctca cgggtcatcg gaatcccgct cgtcgccggc
    47041 ctcgcgttcg aggtcatcaa gctgttcggc cgcaagcgcg acaagcgctg ggcgcagatc
    47101 ctgatggcgc ccggcatgca gctccagaag ctcaccacgc gcgagcccga cctcgaccag
    47161 ctcgcggtcg cgatcgccgc cctcgaggcc gtgctcgcgg tcgagaaccc cgagcaggcg
    47221 accgaggagg accgggtcgg catggaggtc gtggcgtagc gaggccctaa cctcgcctgc
    47281 atgatcgacg acctggtcgg gcagatcgag agccgctaca gggagctctc agcccagatg
    47341 tcggaccccg aggtgatcgg cgaccgggag cgctacgccg aggtgggccg cgcctacagc
    47401 cacatcgagc cggcctacgc gctggcagtc gagtaccgca aggccgccga cgacgccgcg
    47461 ggtgctcgcg agctgctcgc ggagggcgac gaccccgagc tgcgcgagct gctgcgcacc
    47521 tcggaggcgc gcatcgccga gctcgaggag gagatccgcc tggcgatggt ggagcccgac
    47581 ccggccgacg agaaggacgt gatcgtcgag gtgcgcggag gcaccggcgg ggaggaggcg
    47641 ggcctgttcg cgggcgacct ccaccggatg ctcgcgcgct acgccgagcg tcgcggcttc
    47701 aagaccgagc cgctctcgac cgctgacggc gagtacacgt tcgcgatccg cggccgcggc
    47761 gcctacagcg tcttcaagtt cgagggcggg acccatcggg tgcagcgcgt gcccgagacc
    47821 gagtcgcagg gccgcatcca cacctcgacc gccaccgtcg cggtgctgcc ggaggccgag
    47881 gaggtcgacg tgcgggtcga cccgaacgac ctccagatcg acgtgtaccg ctcctcgggc
    47941 ccgggcgggc agtccgtcaa cacgaccgac tcggccgtgc gcatcacgca caagccgacc
    48001 gggctcgtgg tctcgatgca ggacgagaag tcgcagctcc agaaccgcga gaaggcgatg
    48061 cgcgtgctgc gcgcgcggct ctacgagcag gagctggccg cgcgtcaggc ggagatgtcg
    48121 gccgagcgca agtcgcaggt cggcaccggc gagcgctcgg agaaggtgcg cacctacaac
    48181 ttcccgcagg accgcgtgac cgaccaccgc gtgaagctca cgaagggcaa cctgcccgcc
    48241 gtgctcgcgg gcgagctcga cgagctgacg gcggcgctcg aggccgacga gaagcgccgc
    48301 aagctcgagg ccagctcgcg cggggagtga ccaccgcggt cggcacggtc cgcgacgcga
    48361 tcggcgccgc ggcggacgcg ttgggtgcgg cgggttgcga cacgccgcgg ctcgacgcgg
    48421 agctgttggt cgcggcggcg ctcgggatgc cgcgcgaacg gcttcacctg gagcctgaat
    48481 cgggagtgcc accgcgcgcc gctcgcgtgg cctccgagtt cattcgccgc cgggcacggc
    48541 gcgagcctgt cgcgtacatc ctcggccgcc gcggctttcg ccatatcgac gtggccgtcg
    48601 acgcgcgcgt gctcatcccg cggccggaga cggagctgct cgtggaggtc gccctggagc
    48661 tgcccgccgg agcgcgcgtg cacgacgtgg ggaccgggtc gggggcgatc gcgctcgcga
    48721 tcgcggacga gcgccccgac ctcgtggtga gcgcgtccga cgcctcgcgc gccgccgtgg
    48781 acgcggcccg cgcgaacgcc gcgcggctcg ggctcgctgt cgcggtcgag cacgcggcgg
    48841 ggcttccgga gacctcccgc gacgcggatc tggtggtggc gaacctgccg tacgtcaccg
    48901 aggcggagtg ggcggggctc gcgcccgaga tcgtccgcta cgagccgcgc gaggcgctgg
    48961 tctccggcga cgacgggctc gacgcgatcc gcgcgctggt ggccggcgct ccgcgcggtc
    49021 tccgcatggc gctcgagcac gcgccgtcgc aaacggcggc agtgagggag atgctcgcgg
    49081 agccgcgcac gctggaggac ctcgccgggc gcgagcgcgt gacgcgcggc cgggttctga
    49141 acacttaacc acgctatggg gtggtcaagt gttcaatcgt cggatgtgga ggcctttcag
    49201 gccgccctcc tgagtgacgg tgtcggtctc ttccccgccg acacggtcta cggcctcgcc
    49261 gcggacgccg agtccgcgcg cggcgtcgag cgcctctacg agctgaaggg ccgcccgccg
    49321 gtgaagcccg cggccgtcat gttcttcgac cccgacgccg tcctcgacct cgcgcgcgac
    49381 ctcggtcccc gaacgcgcga ggcgctcgcg cggctgctcc ccgggccggt cacgctgatc
    49441 gtcccgaacc ccgagcgccg ctacccgctc gcgtgcgggc ccgtgccgga gcggctcggg
    49501 atccgcgtgc ccggcctgcc ggagccgctc gcggcgctgc tcgccatccg ccggccgata
    49561 ctccagtcga gcgccaaccc gtcgggtgga ccggaccccc ggcgcctcgc cgacgtggac
    49621 ccggagatac gcgccggggt ggacgcggag ctcgacggcg gggagcttcc gggcaccgca
    49681 tcgacggtcg tggacctcgt ccgctacgag caggcggggg agttcgaggt gctgcgcgag
    49741 ggcgccgtgg ccgcggagga gctcgccgag ctcctctgag gcccaccccc cgtgggcacg
    49801 aaatttcagg cgaacaagtt tttgacgcgt ctgggccgga aacattgtgc ttaagcaaac
    49861 gtgtcccctg ccctgggaag gccgccggta gacatccggc gcagtgggga cgcagaggga
    49921 gaaggaaaaa ggtcatggtg cgcgggccaa cctcccgcag ttcctagacg actgttcgag
    49981 ttttcgcccg ggcgatcggg gtgatcgttc gtgaggcaag gactgatgcc gaggcgagtt
    50041 caacagcgac ggtttgaccc ggtcgagacg accgcgacag cccctgcccc cgcccctgct
    50101 cgcgaggcgg gttacggggc gctcgtgctc gagcggctgg cgaagcagat ctgccgcatc
    50161 gccggcgtcg agctctcgtg catcttcgtc cgcgaccttc gcgacccgcg cctggtgatc
    50221 gcggccgccg ggcacggcct cccctgggat gcgatcggct gccgcttcgg tgccgacgag
    50281 ggccccgtcg gccacgtgct cgcgaccggc gagcccacgc tgagcgacga cttcgccgcg
    50341 ctggtcagcc cgctcgaggt tcccgaggga tcgtcgggct gcgctggcgt cgcggtgccg
    50401 gtgcgatgga acggccgggt ggacggcgcg ctcgtggccg gctgctgcac ccccggccgc
    50461 gcatttgggg aggaggagct gctcctgctc tccgagctcg cggacctcgc ggccgcggcg
    50521 ctcgagcaca cgaccgagcg tcacgacttc ggcgacctga tgaaggcgca cgtggaggcg
    50581 ctcgccgccg cgatggacat gcgcgaccgc cgcaccgcgg cgcactcgga ggacgtggta
    50641 cggctcgcct gcgaggtcgg gcgactcctc ggactccagg acgcctcgct cgtcgagctc
    50701 gagttcgccg cgcgcctgca cgacgtcggc aagatccgcg tgcccgacgc ggtcctgcac
    50761 aagccgggcc cgctcgacga ggacgagttc gaggtcatcc gctgccacgc ggcgtggggc
    50821 tcggagacgc tctcggggat ccccgggctc gaggcggtcg cgaccgtggt gcgcttccac
    50881 cacgagcgct gggacggcaa cgggtacccc gacaagctcg ccggcgcgcg catcccgctc
    50941 gccagccgga tcatctgcgc gtgcgacgcc ttcgccgcga tgaccgcgga ccgcccgtac
    51001 cgcgacgcga tggacaacga cgacgcgctg gcggagatct gctcgaagtc cggcagccag
    51061 ttcgacccct cggtcgtgga cgccctcgcc gaggtcgtcg cgaccgtcta gacagcttcc
    51121 ccacaggcgc cggcgctcac cccctcggag cgccggcgcc gaccattccc gcgccccgcg
    51181 cccgctttgc cctcggatgg cgctggtacg gtgactgcgt gaccgatctg ccgcccgact
    51241 tccagacccg cccggtcgcc gaggtcgatc ccgagatcgc gcaggtgctg gaggacgagc
    51301 tccggcgcca ggacgcgaca ctcgagatga tcgcctccga gaacttcgtc ccgcaggccg
    51361 tgctcgactg ccagggctcg gtgctcacga acaagtacgc cgagggctat ccgggccggc
    51421 gctactacgg cggctgcgag tacgtggacg tcgccgagca gctcgcgatc gaccgcgcga
    51481 aggcgctttt cggggccgac cacgccaacg tgcagcccca cgcgggcgcg caggccaaca
    51541 acgccgtcta ccacgcactg ctcacgccgg gcgaccgcgt cctcggcatg aagctcgacc
    51601 acggcggcca cctgagccac gggatgaaga tcaacgtctc cggcaagctc tacgacgtcg
    51661 ccgcctacgg cgtgcgcgag agcgactcgc ggctcgacat ggaggacgtc gcgcgcgcgg
    51721 cgtacgagca ccggccgaag ctgatcgtgg ccggatggtc ggcctacccg cgccagctcg
    51781 acttcgcggc gttccgggcg atcgcggacg acgttggcgc gttcctaatg gtcgacatgg
    51841 cgcacttcgc gggcctggtt gccgccggcc tccacccgag ccccgtcccg cacgcggacg
    51901 tggtcaccac caccgtgcac aagacgctcg ccggcccgcg gagcgggatg atcctgtgcc
    51961 gcgaggagca cgccaagaag atcaactcgg ccgtgttccc ggggcagcag ggcggcccgc
    52021 tgatgcacgc gatcgcggcc aaggcagtgg cgctgcggat cgccgccagc gagcccttcc
    52081 gcgagcgcca gcagcgcacg gtcgacggcg cgaagcgcct cgccgaggag ctgctcggcg
    52141 ccggcgtgaa cgtgctcacc ggcggcaccg acgtgcacct gatcctcgcc gacctgcgcg
    52201 actccgagct cgacggccag caggccgagg accgtctcga cgaggtcgga atcaccgtga
    52261 accgcaacgc cgtcccgttc gacccgcgcc cgccggcgat ctcctccggg ctgcgcgtcg
    52321 gcacgcccgc gctggccacg cgcgggttcg gcgacgagga gttcaccgag gtcggccgga
    52381 tcatcggcac cgcgctcacc gcggagtgga gcgacgacct gcgcaccgag ctcgccgagc
    52441 gcacgcgggg gctcgccgag cgccacccgc tctacccgca gctgctgcaa acggtctgag
    52501 ccgcacgctc ttcgagctcg accgagtcac cgcctcgcgc ggcggggcgg cggtgctgcg
    52561 cgacgtggtg ctggacatac gggagggcgt gacggcgctg ctcgggccgt cgggctcggg
    52621 caagtcgacc ctgctgaggc tgcttaaccg gctggccgac cccgatcgcg gcatcgtgcg
    52681 cttccgcggc gaggacgtga cgatgctcga cccgcgcgag ctgcgccgcc gcgcgtgcct
    52741 cgtgccgcag ctaccggcgc cgctgcccgg cacggtcgcc gacaacctgc gctacgggcc
    52801 ggcgctacgc ggccgcgccg tcggcgtcga gcgcccgctc gagctggccg ggctcacgaa
    52861 gctctacgcc gaccgcgacg cgtcgcagct ctcggtcggc gagcagcagc gcgtgatgct
    52921 cgcccgcgcg ttggcgctcg agccggaggt gctgctgctc gacgagccga cctccgccct
    52981 cgacgactac acgaagggcg gcgtggagcg gacgctcgcc gagctggccc accgctccgg
    53041 gctgtcgatg gtgctcgtga cccacgaccg cgcccaggcc gagcggatgg cgggccggat
    53101 cgtggagctg cacgacgggg cggtggcgcg gtgaggatcg acgtctcgct gctcgaggtg
    53161 ctcgggtcgc tcgcgctggt ggcgctggcg gcgggcatct cgtactggcg gcggatcgac
    53221 atcgagcgcg acctcggtgt ggccgtgctt cgctcgttcc tccagctcac cgcggtcggc
    53281 ttcgtgatcc aggcgatctt cgactccgac agcctcctgc tcgtggcgct gctgctcgcg
    53341 gtgatggtcg cgttcggctc gttcacggcg cgctcgcggg cgcggcaggt gccgggcgcg
    53401 ctgcccgcga tcgcgctcgg gctgagcgcc gccgcggtga tcacgatcgg gctggtgatc
    53461 gggctcggcg tgttcgacgc cgagccgcgc tacctcgttc ccgtcggcgg catggtgatc
    53521 ggcaacgcga tgaccgccgc ggccatctcg ctcaaccggc tggccgacga catgcgcgcg
    53581 tcgcggctcc agatcgaggc gacgctggcg ctcggcgcga ccgcggcgga ggccgcccgc
    53641 gcgatcgtcc aacgcagcct ccgctccggg atgatcgggc tggtggactc caccaagacc
    53701 accggcgtcg tcttcttccc cggcacgatg gtgggcatgc tggtcgccgg cgccgacccg
    53761 gtcgacgccg tgcggctcca actcatcctg ctctggacgc tgatcgggag cgtggcgatc
    53821 tcggctgtga tcgcgacgtc cctcggctac cgcggcttct tcacccccgc gcaccagctg
    53881 cgcgagctgc cggctagccg cccggacgct tgacggcctc gaactccccg gtctcgagct
    53941 cgtgcgccac gcgcgcctcg atgtcggcct cggccggttc cggcgcgccc gcgagcttgc
    54001 tctcgttgcg gagctggaac tggcggaagc gcttgagctt gaggatctcg agcacgtaga
    54061 ccaggtacgc gctcgcggcg accgccacga ggccgaagcc ggccagcacg gcgacccact
    54121 cccagtcgaa gtggccgtgg tcgtcggagt aggggacgaa ccgcagcgcc agcgcgagcg
    54181 cggccagcga gagggtccag ccgtagaggt agagggcggt ctttcgctgc gagaaaccga
    54241 tgttcgcgaa gcggtggtgg aagtgccagc gatcggcctt gtagaccggc tggcggtact
    54301 tgaggcgctt ggcgatcacg aagctcgagt cgaggatcgg cacggcgagg atcaccatcg
    54361 ggaacgccag cgcgaccacg gcgttcgtct tcagcgctcc ctgcaccacg atggcgccga
    54421 gcaggtagcc gagcaggttc gaccccgagt cgccgaggaa caccgaggcc gggtggaagc
    54481 cgtggcgcag gtagccgagc gaggcaccgg cgacgatcag cgcgagcacg cccgcgacct
    54541 ggcggtcgag cgacagcgag atcgtggcga aggtgagtgc ggcgatcgtg cacacgccgg
    54601 cggcgagccc gtcgacgccg tcgatgaagt tgacgacgtt catcagggcg acgatgccga
    54661 gggccgtcag gccgtaggac agcgggtcgc tcaggtcgat ccgcccgaag aacgggatcg
    54721 tgaagtcggt gacgcgcacg ccggcggcga ccggtatcgc tgcggcgagg atctggccga
    54781 cgatcttgta ctcggccgag agctcgagga tgtcgtcggc catcccgacg gcgacgatca
    54841 ccgcggcgcc gatcaggatc gcgttggtct gctggtcgtg ggggaggaag acgagcgtcg
    54901 aggtggcgac cgccgcgagg atcgcgaggc cgccgagccg gggcgtcggc atctggtgca
    54961 ggccgcgcgc ccgcggggtg tcgatcgcgc cgatcctcca ggcgagggca cccgcgatcg
    55021 gggtggtcag cagcgcgacc accagcgcga ggccgagggc gacgaaacca tcgccggtgc
    55081 taggcatggc acctcactgc ttcgaccgta gcgcgcgagc ccctgcgtga cgctcgtgcc
    55141 acagcccgaa cgacatgagg ccccagagct ggcggctcag gtcctcgcgg ccggagacgt
    55201 ggcgatcgag gatcgcggtg acggtcgcgg ggtcgaagaa cccctggctg cggaccgtgt
    55261 cgggggagag cacctcacgt gcgaacggct cgagctcgcc gcgcagccag gcggccgccg
    55321 gtatcgagaa gccgcgcttg cggccgcggt ggatctcgcg cggcacgagc ggcgccaccg
    55381 ccttgcgcag caggcgcttc ttctggaggc cgcgcacgcg catgcggcgt gggagtgcca
    55441 gggcgaagtc ggccagcgcc gtgtctagga acggcacgcg cgcctcgagc gagtgcgcca
    55501 tgctcgcgcg gtcggtcttc gtgagcaggt cgtcgggcag gtagatcccg acgtccacgt
    55561 cctggaggcg cgcgatcgcc tcggagccgg cggtctcggc gtagcgggcg cggtccacgt
    55621 cgagcgggtc gcgcacccct gtgcgccgcg ggccgagcag ctccgcccgc gcctccggcg
    55681 cgaagatctc cttccagccg tggtggcgct cgagcggcgg cagcgcgccc gcgcgcgcga
    55741 agcgcttggc catgtagtcg aggctcgtgc gccgcgacga gctgggcagc cgctcgacca
    55801 gcggacgcag cgcggcgact ggtcggccga cgaccggcgc gatcaggtcg gcgacgtagg
    55861 tgaagtagcc gccgaacagc tcgtcgccgc cctcgcccga gagcgcgacc ttcacgtgct
    55921 cggaggcgag cttcgacacc agataggtgg gcagcgccga cgagtccgcg aacggctcgt
    55981 cgaagaactc cgcgatctcg tgcatcagct cggcggcgtc cgggcgcacc accagctcgt
    56041 ggtgctcggt gccgtagcgc tcggcgacca gccgtgccag gtctagctcg ttgaacgagc
    56101 gctcctcgaa gccgatcgag aaggtgttca cgcggccgct cgactcctgc gaggcgagcg
    56161 cggcgagcgc cgaggagtcg accccgcccg agagcatcac gccgaccggc acgtcggcaa
    56221 cgaggtgggc gcgtaccgag tcgcgcaggc gctcgcgcag ctcctccgcc aactcgtcct
    56281 cggtgccgcc gagttgctcg cccgcccgcg cggggccggg gcgcgcgtag cgctcgacgc
    56341 gcgttgcgcc gccctcccaa acgagcaggt gcccgggcgg cagcttgcgc gcctcgcggt
    56401 agatcgtgag cggcgccggg atcgagttga aggcgaggaa tgcctcgacg gcgtcgaggt
    56461 ccacctcccc cgagagctcg ggctggcgca tgagcgcctt gagctcggag gcgaacgaga
    56521 gcacgccgcg ggccgtcgcg tagtacagcg gcttgatccc gaagcggtcg cgagcgagca
    56581 cgagccggcg ccggccggca tcccacagcg cgatggcgaa catcccgcgc aggtcctcga
    56641 cgaagcgcgg cccgcgctcc tcgtagaggt gcacgagcgc ctcggtgtcg gagtgggtcg
    56701 agaaccggtg accgcgctgc tcgagccccg ccatcagctc gcggtagttg tagatctcgc
    56761 cgttctggat cacgtggacg ctgccgtcct cgttcgagat cggctggtcg ccgccggcga
    56821 ggtcgatgat cgacagacgc cgcatcgcga gcccgacggg accgtccagc agcatgccgt
    56881 cgctgtccgg cccgcggtgg aacagggcct cgttcatcga ctggagcgcg gccggatcgc
    56941 ggcccgtcac gccgtcgagc gacgcctggc cgcagatgcc gcacatgggc cctgactcta
    57001 gctctggagc gccttgcgcc cggtaaacgg gcggctcgcg atgcgccaga gcgtctcgag
    57061 caccacgcgc atatagaggt gcaccgagcg gtccgagatg tactcgtagt cgtgggccag
    57121 cttctcggcc caggagtgct cgcgggtgag gcgcatctgc gcgaacccgg tcatcccggg
    57181 gcgcaccacg aggcgctgcc agtactgcgg gatctcgacg cacagctcct cgaagaacgc
    57241 gggccgaatc gggcgcgggc cgacgatgct catgtcgccg cgcagcacgg tccagagctg
    57301 gggcagctcg tcgagctgcg aggagcgcag cacgcggccg accgccgtga cctcgtcctc
    57361 ggtgagcttc gagagcgtgt tgccgaggta ggggccgagc cgcacctcgg cgtccggcac
    57421 aagcgtgcgg aacttgtaca tcgtgaagat ccgcccggcc cggcccacgc gccgcccgcg
    57481 gtagaggacc ggctcgcccg aggtcagccg gatcgcgagc gcgatgctcc cgatcagggg
    57541 gctggtgagg gcgagcgtgc cgcccgcgat ggccacgtcg aggccgcgca ggacggcatc
    57601 caccgggcgc ggctcgaggt tggcctcgtg tgccagccgc gcgtagcgct cggcgagcga
    57661 gccgggccgt accgcttcct gagtggcggc ctccatctca aggcgagtgt gaccgattca
    57721 gcggatcgct gacgagtcga gcgtgccgag gaacatgtcc accagcacct cggggtcgcg
    57781 ccgctgcgcc tggcccgcgc cgtccttctt ctcgatctcg atggcgcgcg ggtccgtgag
    57841 cgggcgcagc cggttctcgc ggatcagggc ctcgtccacg ccgccgagcc ggccgccgta
    57901 ggtggtgtag accggcgtgc cgagcgcgac cgcctcgcgg ttcatggtgc ctccggcgga
    57961 gatcacgagg tcgccgagcg cgatcaggct ctgcgcgtcg acggcgccct cgggcacgat
    58021 caccgacggc agcccgagct cgcggacgta gctgcgctgc gcctcggtgc gcgggatgac
    58081 caccgcctgc acgccgtcgt gacggcctag gtggtcgagc acctggggga agagcgggtt
    58141 cgacttgcgg tggtagagcg acacgtcggg cggcgggcgg aagatcacga cgaccctgtc
    58201 gcggtccacg ccgagctggt cgagcacgcc cgcgtccggc tcgaagtcgt gcaggtagta
    58261 ctcctccttc agccccgggt actggacgag cttgtccggg ccgacgccgt agcgggcgag
    58321 ccgctccgcg gggatgcggt cggggaccat cacccggcgc gcgaggcggc agccgacgtt
    58381 gtgctgctgg acggcgaact cgtagtcgaa cgtgttcgcg gcggggatgc cgagcgtggc
    58441 ggcggcgagg gcgaggtcgt tggagccgtg ggcgagggcg aggtcgaacc ggcggcccgc
    58501 gccgaagcgg cgcatcgcgc cggtgcgcct ggcgagcgcg accgccttct gcgcgcgggc
    58561 ggcgccgccg tggcggccga acgcggtgtg gtcgatgcgg tgcatctgga gcagctcgag
    58621 cgtctgcgcg tagtcgcgcg cggtgacctc cacctcgtgg ccgcgggcgc gcagccgctc
    58681 gatgatcggc cggaacacga gcacgtgcgc cggcgccgtc atgtcgatcc agatccgcac
    58741 gggacccgac cctagaacag gctcggctgc tcgggcgcgc gggcggcctg cgcctccttc
    58801 gcgcggcgct cggcctcccg ctcgaccgcc gcctggcgcc attcgcgcgc gcggcgctcg
    58861 tcgcgctcga ggttgcgccg cgtgaacttc gagcgctgcc tgacgagcga ctggatccgc
    58921 tccttctccg ggcccgccag gtaagcgccg cgggcgtaga gctgctcgta gcggtccacg
    58981 aggtccggcc ggtacgagcg cagccagtcc atgaacacgt cccggacctc gccgcgcagg
    59041 tgcagcccga tgccgccgat gctcaccgcg cccgcctcgg tggcgatctc gatgatcctc
    59101 tccacctgct tggggtcgtc gttgatgccc ggcatcagcg gcgcgatcag gatcccggtg
    59161 gggatgccga gccggttgag ctcggccacc gcctcgagtc gcgcgcgcgg gtgcggggtg
    59221 tgcggctcgc tcgcgcgcca cgccttctcc tcgagcgtcg gcaccgagaa gctcgcgctg
    59281 accggggcgc gctcgttgat ctgtacgagc aggtccttgt cgcgcagcag cagcggcgac
    59341 ttggtgagga tcgagcacgg gttggcgaag tcgcgcatcg cctcccagat gccgggcatg
    59401 agcttgtagc ggccctcgac ccactggtac gggtcggtgt tggtgcccat ggcgacgtgc
    59461 tcgcccttcc acgacgggcg cgcgagctcg cgccgcacga gctccggcgc gttgaccttg
    59521 accacgatct ccttctcgaa gtcgcgaccg ggattgaggc cgaggaaggt gtgggtgggg
    59581 cgggcgaagc aattgtgcga aacgacgccg ttcgcgatga agtcgccggt gccggtagtg
    59641 atgtcgtaga ggggcatgta gggcagccgc tcgatggagg ccactcgtag gttcgagaac
    59701 gtcttgacca tccgcccctg aatcgatcgc tttcgcgtga tcgcgggatc ggtcgtgtgg
    59761 aagaaccgga gcgcctcgga gaggccgccg cgaatgcgaa cgtttctaca cccgttcggc
    59821 cgcgggtctt cgatcacgtg ttgaaaaccg agccgatcga gacacgcagc cgtccagttg
    59881 atgagctccg ggtcggagtt cgcgatgcga atgcactgcc cgaaatggcc ctcggcgtcg
    59941 aagattcccg cgaggaaccc cttgcaccac gcgcggctcc ccgcggcggg ccagtcgatc
    60001 gtgttcacga tggtctcgag acgcgggccg ccggttgctc tgatcgcgcg catcggccga
    60061 tagccggcgg gtaccttgaa ctcgaactcg gccgtctgca ccgccatgtc cgcgagatag
    60121 cgtttggtcc tttgcagcgc ttcgaagtcg gcgagggcga gccggaatac gttcgtacga
    60181 cccactgggc ggccgatgac tcgatgggtg tgcctgacgt gagcatcgcc gcggatgact
    60241 ccagtcaggt agccgcgacg gtaatcctcg gaatcctcgg gcggctccgc gaacttcccc
    60301 gtcccgacca ggtggttgtt gacggtgagg tgcggcctgt cggcctctga gcgagaagtg
    60361 ttcgtcacgt gcttccagcc gcggttgctg agaaagcggt ggtcaccgct ggtcacgagc
    60421 acggtgccgt cctcgagcgt gacccgaaaa gccgggcgca tcaccttcca gtgatcaagc
    60481 acttcggtga cggcataacg cctgtattgc ccttcgcgcg ccgtcccgta gattcggtcg
    60541 ccgacccgca agtcggcaag cagctttgtc cggccggtcg ccataagcac cggcgtctcg
    60601 ccagctccgc aatacgagca tgcgtgtgtg catccgcgat aggggttgat cgtccaccgg
    60661 aacggcatcc gcgagcgctc cggcacgcgg ttgatcgcgg acttcgcgcg gatctcgtag
    60721 aagcgcgtct cgagggcctc gggtgcatcg aaggtgcgca cggtcgcggg atccctgaac
    60781 cccggcagcg cccgccgctc ctgctcctcg accttcagct cgtcccagcg catccgaacg
    60841 tatgttcgca cacccggcgg gcaagtgggc aatcgctgta gaagcgggtt acgaactcgg
    60901 cacgtaccag cgcagcggtc ccgccggtgc cgtgtccagt acgtcttccg gttcgtcaac
    60961 taaggacgaa ccgctagagc aagtcgagct ggtcgatcag gacgtggcct gcgtcgtcca
    61021 gccgacccgc cagcccaacc gtgctgccct cgcgcaggcg gtcggcgcac tcctgcgcct
    61081 cagtgccgaa ggtggtcacg tccacgtaga cgacacccgg ctcgcgccga ccgtggcgtg
    61141 agcgtcgggg gatcgcaagg cgcatcgtgc attggggcac gtcggcgcgg ctggtccgta
    61201 gctgcggctc cccgaccacc tgaccgatca ggctcacgct gttcatgcga gtaacacgat
    61261 tgggctgtag accaaggtaa cacggttagg ctgtagtacg tcgggtggcg cgcaagccgg
    61321 atcggctgtt ggtcggactc gggaaggcga tccgcgagct acgcgccgag cgcggctggt
    61381 cgcaagagga actgagcctc aagaccggcg tacaccggaa ctacatcggc ggcatcgagc
    61441 gcgctgagcg ccagccaaca gtcgagaccg tggcgaagct ggcggcagcg ctggacgtga
    61501 ccccggccga cctcttggcg cgcgcggagc gggcctaggg ggcgtcaggc ggcggcaggt
    61561 ccaagggcat ctggacctgc ctcgggatgt gccggatgac ctcggtgacg tagtagtcgg
    61621 tgtgcaaggc atcgccctgc tgcgactgca cgatccggag gcgacattcg aggaagtcgc
    61681 ccttgcgaaa gaactcgatg cccttctcca cgcggtccac gaagagcggg tcttcgatgt
    61741 tcgcgtagaa cgttcgctcc ccgtcgctga accgccactt gttgtccttg atgaaggcga
    61801 cggacgcgat agcgaggacc atttcgcttt cgcgctccaa gagcggcacg tcgatgtcct
    61861 cgggaggctc gaacgacgtc acgtctgacg atgagatgct gaccgtgatg tcttcctccg
    61921 tgcggagttc gagcctttct acgccccgac ggttcaatgg ctcgactacc tgtcgcgccc
    61981 gcttgcgcac ctggacgttt ctgtagaggt caaaaacctc ggtaggaact tcaagcgtct
    62041 caccgtcctc tagcgtcagc ctgacgttgc cgcgctcgac ctgttcttgg cctttgatga
    62101 gccggcgctt gaccatccgg atgaaggcga acagcccgta ggtgccgccg acgatctgca
    62161 tgaggttggc gagagcgtcc gccgcgtcct tgttcagcag gtccactacc gaatcccaaa
    62221 ggcccgcagc ctcaaggatc aactggacct cgaacgagcc ctcag
//
LOCUS       DASVPL010000252        12483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_109122, whole genome shotgun sequence.
ACCESSION   DASVPL010000252 DASVPL010000000
VERSION     DASVPL010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12483
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109122"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..586)
                     /locus_tag="VF517_13805"
     CDS             complement(53..586)
                     /locus_tag="VF517_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8054061.1"
                     /translation="MSVATETAPPPAETARVAESFEDLYRRTFPRVYAYVASLVRDRS
                     AAEEVTAQAFERAYRRRSSYRPGRGPIEAWLFGIARNAALDELRRARRRARLEDEAAV
                     PAGMAGDEETDGLLRRESVRAALASLDARERDLIALKFAGGLSNAEIARVLGMSESNA
                     GTRLHRTIEKLRRACHD"
     gene            complement(616..1512)
                     /gene="gluQRS"
                     /locus_tag="VF517_13810"
     CDS             complement(616..1512)
                     /gene="gluQRS"
                     /locus_tag="VF517_13810"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX8054062.1"
                     /translation="MDGRYAPSPTGSLHLGNLRTALLAWLFARSAGSRFLMRVDDLDR
                     QRVRPGVEEEQLANLAAIGLDWDGPVVRQSERLALYDEALARLSAEGRVYTCYCTRAE
                     VRAAASAAHGPPVEGFYPGTCRGLTAAERAEREASGRRPALRLRAGGERIAFEDRVLG
                     PREGPVDDFVLRRGDGTAAYNLAVVVDDAAQGVGEVVRGADLVDSTPRQILLARLLGV
                     DAPAYAHVPLVLGPDGTRLAKRHGSVTLADRLALGESVADVVSWMASSAGLAEPRERV
                     SAASLVERFDPDRLAREPTTFV"
     gene            1533..3941
                     /gene="ligD"
                     /locus_tag="VF517_13815"
     CDS             1533..3941
                     /gene="ligD"
                     /locus_tag="VF517_13815"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX8054063.1"
                     /translation="MADKLATYKDKRDFGKTKEPAGGRRKKAAKAPRFVVQEHHARRL
                     HWDLRLERDGVLASWAIPNGIPQDPKQNRKAVHTEDHPIEYIDFEGEIPKGEYGGGTM
                     KVWDTGTYEAEKFRDDEVIVTFHGERLTGKYVLFRAGKDEKDWMIHRMDPPADPDREP
                     MPERLVPMLATAGALPRDEEKWSFEIKWDGVRALVYSEPGRLRIEGRRLTDMTERYPE
                     LRPLNRQLGSRDAILDGEIVAFDENGRPSFERLQHRMHLTGDSRVKRRAKEIPVVLAI
                     FDLLYLDGRNLMGLPYEERRAKLDELGLEGPNWRTPAAHPGEGTALHAASEQQGLEGI
                     VAKRLDSSYEPGRRSNAWIKIKNKRRQELVIGGWIPGEGKRESTIGALLVGYHTPDGS
                     LRFGGRVGTGFTQKALRDLEERLDPLRRKTTPFEGTPKPPKGSVFVRPELVCEVEYTE
                     WTNEMVLRHPAYKGLCSSMEPGEVVLGEEEVVEEVPEAADQGVLGGLEKLQGNAFEAT
                     VDGRKLRLTNIDKVLYPKAGFTKGQVIDYYARIGPVLLPHLSGRPLTLKRYPNGVEGE
                     HFYEKQCPSHRPDWVETASIWSRHNKHNIDFCLANDLPTLVWAANLADLELHTSLSMA
                     AEITRPTMMVFDLDPGEPANIVDCCRVGLWVRELFEGLGMEIFAKTSGSKGLQAYVPL
                     NVETSYDVTKPFSRAVAELLEKQHPEHVVSRMTKSLRPGKVLVDWSQNDEHKTTVNVY
                     SLRAKERPTVSTPVSWDEVAECLRTEDPNVLTFPSDQVLARVDELGDLFAPVLTLKQE
                     LPSF"
     gene            4426..5523
                     /locus_tag="VF517_13820"
     CDS             4426..5523
                     /locus_tag="VF517_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054064.1"
                     /translation="MTRVRTGQMHLRAALILSLVALAALPSAASAALAGPSQTEGSDI
                     FFSAGPGEANRLTVTGQGSTVVFEDAGASIAPGRGCTALAANRVACPRSSFMFVRLED
                     GDDEVRTSGELADTFVFIEGGQGADTIHGEVGDTSIEGGTGPDRLFGGPLTSRLDAVD
                     VVRRGEFEDVPDHNRERDEVTCAQSTRVDLPLRVEVDANDVLAGPCPPHYLFLETFIV
                     IEGTAGADSLTSAGPPTHVNGLAGDDTLSGEQDDRLDGGEGADRLFGQGLLLGGSGDD
                     RLDGGQSSGLTSGARLDGQSGDDEITGTRLGDSITGGSGRDTISSGRGRDSIRARDGA
                     RDRITCGPDRDRVSADRGDTVSRDCEVVSRG"
     gene            5516..6124
                     /locus_tag="VF517_13825"
     CDS             5516..6124
                     /locus_tag="VF517_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054065.1"
                     /translation="MARRLALLTALCLAGSLAAALPAQASHGCSAWRSKGAHLIVKNR
                     EAVVFSKGVYYYGCLSSVGTIRRLPEEGGGIDTGGADAPQLAGRYVAYSTSGSGIGDE
                     FDRIYVYDLRVGRRFLIESSTFIRDIALKRNGSVAWIEAAPADPGDTPVWDVRAWSNE
                     DRQGSVLLDRGTDVGPESLVLTPDRLRIEWTRGGVARSAPLR"
     gene            6156..8312
                     /locus_tag="VF517_13830"
     CDS             6156..8312
                     /locus_tag="VF517_13830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX8054066.1"
                     /translation="MAHSRPAAPVPEADALHAADARLRHLGAITEAALAHLDLDRLLD
                     ELLTRLRGILSVDTIAILLLDPDSNELVARAAKGLEEEVEQGVRIPVGAGFAGRVAAD
                     RRPIYLPKVDRHSVINPILINKGIASMLGVPLLVEGEATGVLHVGSLTPREFGADDAE
                     LLQLAADRMALAIDHARLYASEREARAAAEQRANQLTQLQAITDVALGRLSVDEDVLG
                     DMLERVRDVLAVDTVAVLLLSAEGDELVARAARGLEEEVERGVRIPLGRGFAGRIAEQ
                     GEPLVLEDIDRADLVNPLLREKGIKTLVGVPLLVEERVIGVLHVGSLHPRVFAEEEIG
                     LLERAADRIALAADRARQHTLAGLLQRTLLPARLPEVPSLRMAARYVPGSDEAHVGGD
                     WYDVVELPHGRVGVAIGDVVSRGVRAAAVMGQMRTALRAYALDADGDRPGPVLDRLDR
                     LVRSLDEREMATVAYVVIDPAESVLTASLAGHPPPLVLGADGSTRFLDGVRSRPIGVV
                     AARRYDETTAPLERGETLILYTDGLVERRGVPIDEGMRRLEEVAASVAGADPDELCDR
                     LVRLAEDDSVADDVAVLAIRLPAAAAESLAMTLPAEPGSLSTMRRAVRDWLGRREVDP
                     AVGNDILVAMGEAAANAVEHAYGPGNAEFRLEARIDAGEIVVRIYDEGRWRPARGSHR
                     GRGLSMMEALMEDVQVDSDESGTVVTMRRKLEGAGP"
     gene            8309..8665
                     /locus_tag="VF517_13835"
     CDS             8309..8665
                     /locus_tag="VF517_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8054067.1"
                     /translation="MIAGSTPPQITIDEGVVIAALPDEIDLANARGITSMVSAAVPNE
                     AVGVVLDLSGTTYLDSSGVHLIFDLADRLGARQQRLVLAVPETSNVRRVLELVDVTVV
                     AAMVPELARAKELARG"
     gene            8714..9568
                     /locus_tag="VF517_13840"
     CDS             8714..9568
                     /locus_tag="VF517_13840"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX8054068.1"
                     /translation="MTRTGTQALTLSAEIRARFEEFSRSQKDVGQYIVDHLDEAAFHT
                     AEELARRANTSSSTVVRFAQALGFEGFPELQASAREEYRRARDSGGPVGDPLETQPLF
                     PIDQTEFEAALAADHVNVAETARKLDRDEVAAAVERISRSEHTLLCGTDQMAFFASYL
                     RHLLMLLDLRCEVVASPSQEGLARLGRIDDRTLVIGFSAGRPHPLVVRAMKLARHRRA
                     GTIAIADATLSEVAKLADQRLYYSSNSPAFVRSHTALLGMIQALAYGVYALDESAYAD
                     RIKAFRLK"
     gene            9712..10596
                     /gene="pdxS"
                     /locus_tag="VF517_13845"
     CDS             9712..10596
                     /gene="pdxS"
                     /locus_tag="VF517_13845"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX8054069.1"
                     /translation="MKDTATFRVKAGLAEMLKGGVIMDVVTAEQARVAEAAGAVAVMA
                     LELVPADIRAAGGVARMADPTKIEEIQGAVTIPVMAKARIGHFTEAQVLEALEVDYVD
                     ESEVLTPADEANHIDKWKFKVPFVCGATNLGEALRRIAEGAAMIRTKGEAGTGNVVEA
                     VRHMRTIRGDIRRLSSMDESELASAAKDLRAPLELVRAVAEKCGLPVVNFSAGGIATP
                     ADAALMMQLGAEGVFVGSGIFKSEDPERTAKAIVEATTHYDDPERVARASRDLGAPMG
                     GIEISSLGEDGLLQTRGW"
     gene            10601..11167
                     /gene="pdxT"
                     /locus_tag="VF517_13850"
     CDS             10601..11167
                     /gene="pdxT"
                     /locus_tag="VF517_13850"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX8054070.1"
                     /translation="MVVGVLALQGDFEAHAGMLTRLGAEARIVRTPGELAGVDALVVP
                     GGESTTITLGIEREGLAEPLRELVSGGTPVLATCAGMIVLDRDHLGVLDLTTRRNAFG
                     RQVMSFEADLDLPGLGETPLRAVFIRAPWVEEHGANVEVLAEVDGHPVAVRQGEVTAV
                     AFHPELTSDARLHRWLVEAAGARIKEPA"
     gene            11164..12267
                     /locus_tag="VF517_13855"
     CDS             11164..12267
                     /locus_tag="VF517_13855"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX8054071.1"
                     /translation="MKDQRAEALAQILVRYSTKVQKGDTVVIQSTTPAEPLALAVYEE
                     VLRAGGLPMVQLAPDGAASAFYGLASDEQLDWVSPLSRWIAEESDVRISLMADSNARE
                     LSQADPKKQARAQKARRSLLDTTMKRAASGEHRWALTLFPTHAYAAEANMSLAQYEDF
                     YYAACLATDPEPVTAWARQSDEVKRLAEWIQGKEEVHITAPGTDIKLGVAGRRFIPCT
                     GEHNMPDGEFFTGPIEDAVDGEVSFSFPASYGGREVTGVRFRFEGGKVVDASAERGED
                     FLIEMLDTDDGARRLGELGIGTNYGIADGTKEILLDEKIGGTVHMAIGASYPESGGVN
                     ESAVHWDMVCDLRQGGAIVVDGVELQRDGRFVV"
BASE COUNT         1834 a         4557 c         4471 g         1621 t
ORIGIN      
        1 ggccaacgcg tcgtcgacgg ccgccagcgc gacgtcccgg tcgagctcgt tctcagtcat
       61 ggcaggccct ccgcagcttc tcgatggtcc ggtgcagcct ggtcccggcg ttcgactcgg
      121 acatgccgag cacgcgagcg atctccgcgt tcgacagccc gcccgcgaac ttcagcgcga
      181 tcaggtcgcg ctcccgcgcg tcgagcgatg cgagcgcggc gcgcacggac tcgcggcgca
      241 gcaggccgtc ggtctcctcg tcgcccgcca tcccggccgg cacggcggcc tcgtcctcca
      301 accgtgcgcg acgtcgcgcg cgccgcagct cgtccagcgc cgcgttgcgc gcgatcccga
      361 acagccacgc ctcgatcgga ccgcgccccg gccggtagct cgaccgccgc cggtacgcgc
      421 gctcgaacgc ctgcgcggtt acctcctccg cggccgagcg gtcgcgcacg agcgacgcca
      481 cgtacgcgta cacgcgcggg aacgtgcgcc ggtagaggtc ctcgaagctc tccgccacgc
      541 gtgccgtctc ggccggcggc ggggcggtct cggtcgcgac gctcacaccc ctactacggc
      601 gccgcggccg atccatcaca cgaacgtggt cggctcgcga gccagccggt ccggatcgaa
      661 gcgctcgacc agtgaggccg cgctcacccg ctcgcgcggc tcggcgagcc ccgcgctcga
      721 cgccatccac gacacgacgt ccgccacgct ctcgccgagc gccaaccggt cggccagggt
      781 gaccgagccg tggcgcttcg cgagccgggt cccgtccggc ccgagcacaa gcggcacgtg
      841 cgcgtacgcg ggcgcgtcga ctccgaggag gcgcgcgagc aggatctgcc gcggcgtcga
      901 gtcgaccagg tcggcaccac gcaccacctc gccgacgccc tgggcggcgt cgtccacgac
      961 caccgcgagg ttgtaagcgg ccgtcccgtc gccgcgccgc agcacgaagt cgtcgaccgg
     1021 accctcgcgg ggcccgagca cccggtcctc gaacgcgatc cgctcgccgc ccgcgcgcag
     1081 ccgcagggcc ggccgtcgcc cggacgcctc gcgctcggcg cgctccgcgg cggtcaaacc
     1141 ccggcaggtt cccgggtaga acccctcgac cggcggcccg tgtgccgccg aagcggcggc
     1201 gcggacctcc gctcgcgtgc agtagcacgt gtacacacgc ccctcggccg acagccgcgc
     1261 gagcgcctcg tcgtagaggg ccagccgctc cgactggcgc accaccggac cgtcccagtc
     1321 gagcccgatg gccgcgaggt tcgcgagctg ctcctcctcg acccccggcc gcacgcgctg
     1381 gcggtcgagg tcatccaccc gcatgaggaa ccgcgacccc gccgaccgcg cgaacagcca
     1441 cgccagcagg gccgtgcgca ggttcccgag gtgcagcgac ccggtcgggc tgggcgcgta
     1501 gcggccgtcc acggcggata ccgtctcagg cgatggcgga caagctcgcc acctacaagg
     1561 acaagcgcga cttcgggaag acgaaggagc ccgccggcgg caggcgcaag aaggccgcca
     1621 aggccccgcg cttcgtggtc caggagcacc acgcgcgccg cctgcactgg gacctccggc
     1681 tcgagcgtga cggcgtgctc gcgagctggg cgatcccgaa cggcatcccg caggacccca
     1741 agcagaaccg caaggccgtt cacaccgagg accacccgat cgagtacatc gacttcgagg
     1801 gcgagatccc gaagggcgag tacggcggcg gcacgatgaa ggtctgggac accggcacct
     1861 acgaggccga gaagttccgc gacgacgagg tgatcgtgac cttccacggc gagcggctca
     1921 ccggcaagta cgtcctgttc cgcgcgggca aggacgagaa ggactggatg atccaccgga
     1981 tggacccccc ggcggacccc gaccgggagc ccatgcccga gcgcctcgtg ccgatgctcg
     2041 ccacagccgg cgccctcccg cgcgacgagg agaagtggtc gttcgagatc aagtgggacg
     2101 gcgtgcgcgc gctcgtctac agcgagcccg gccgcctgcg catcgagggc cggcgactca
     2161 ccgacatgac cgagcgctat cccgagctgc gcccgctcaa ccgccagctc ggctcgcgcg
     2221 acgcgatcct cgacggcgag atcgttgcct tcgacgagaa cggccgcccg agcttcgagc
     2281 gcctccagca ccgcatgcac ctcaccggcg actcgcgcgt caagcgccgc gccaaggaga
     2341 ttcccgtcgt cctggccatc ttcgatctcc tctacctcga cggccgcaac ctcatgggcc
     2401 ttccgtacga ggagcgccgc gcgaagctcg acgagctggg cctcgagggg cccaactggc
     2461 ggacgcccgc cgcgcacccg ggggagggca ccgccctgca cgccgcgagc gagcagcagg
     2521 gcctcgaggg gatcgtggcg aagcggctcg acagctcgta cgagccgggc cggcgctcga
     2581 acgcgtggat caagatcaag aacaagcgcc gccaggagct cgtgatcggc ggctggatcc
     2641 cgggcgaggg caagcgcgag agcacgatcg gcgcgctgct cgtcggctac cacacgccgg
     2701 acggcagctt gcgcttcggc gggcgcgtag gcaccggctt cacgcagaag gcgctgcgcg
     2761 acctggagga gcgcctcgac ccgctgcggc gcaagaccac cccgttcgag ggaacgccca
     2821 agccgcccaa gggctcggtg ttcgtccgcc ccgagctggt ctgcgaggtc gagtacaccg
     2881 agtggaccaa cgagatggtc ctgcgccacc ccgcctacaa ggggctctgc tcgagcatgg
     2941 agcccggcga ggtcgtgctc ggcgaggagg aggtggtcga ggaggtgccg gaggcggccg
     3001 accagggcgt gctcggtggc ctcgagaagc tccagggcaa cgcgttcgag gcgaccgtcg
     3061 acggccgcaa gctgcgcctc acgaacatcg acaaggtcct ctacccgaag gccggcttca
     3121 ccaagggcca ggtgatcgac tactacgcgc ggatcgggcc ggtgctgctg ccgcacctga
     3181 gcggccgccc gctgacgctg aagcgctatc cgaacggcgt cgagggcgag cacttctacg
     3241 agaagcagtg cccctcgcac cggccggact gggtcgagac ggcctcgatc tggagccgcc
     3301 acaacaagca caacatcgac ttctgcctgg ccaacgacct gccgacgctc gtctgggcgg
     3361 cgaacctcgc cgacctcgag ctgcacacgt cgctctcgat ggccgccgag atcacccgcc
     3421 cgacgatgat ggtcttcgac ctcgacccgg gcgagccggc gaacatcgtc gactgctgcc
     3481 gcgtcgggct ctgggtgcgc gagctgttcg aggggctcgg catggagatc ttcgccaaga
     3541 cctccggctc gaagggcctc caggcctacg tcccgttgaa cgtcgagacg agctacgacg
     3601 tgacgaagcc gttctcgcgc gcggttgcgg agctgctcga gaagcagcat cccgagcacg
     3661 tggtctcgcg gatgacgaag agcctgcggc cggggaaggt gctggtggac tggagccaga
     3721 acgacgagca caagaccacc gtgaacgtct actcgctgcg cgcgaaggag cgcccgacgg
     3781 tctcgacccc ggtgagctgg gacgaggtcg cggagtgcct gcgcacggag gacccgaacg
     3841 tgcttacgtt cccctccgac caggtgctcg cccgcgtgga cgagctgggc gacctgttcg
     3901 cgccggtgct gaccctgaag caggagctgc cgtcgttctg agacttggaa ggccacgcaa
     3961 ggaggcgcgg agcttcagcc ggcggctggt cgacgaggag tcgcgcgcgt tcgtcgagga
     4021 ggagctcgag gagacgcgcg agctctaccg cacgggcagc ggggagttcc tgctcgagcc
     4081 gctctgccgg cacgtcttcg cgagcgcggc gaccacggac atccagtggc tcaagagccg
     4141 cggcatcgac ggcgagcgct tctaccgcga ggagctggcg ccgagctggg agggcctcga
     4201 ccgcgaggag cgcgtccggc ggatcgagcg gttcatcgag ctgtcgggcc agctcgggtc
     4261 ggtgagcggc aacggaacgg cccccgggcc agaggtgcgc gacctcgtcg ccaccgtgca
     4321 cgtgaaggtg atcctgctcg cctgggcctt cgaccgcacg cacggcttcg tcgaccgcct
     4381 cttcaacggt ccgctccagt atcggctgca ccgcaccaac tccgcgtgac gcgggttcgt
     4441 accgggcaga tgcacctgcg agcagccctg atcctctccc tcgtcgccct cgcggcgctg
     4501 ccctcggcgg cgagcgccgc cctggcgggc cccagccaga ccgaggggtc ggacatcttc
     4561 ttctcggcgg gccccggcga ggccaaccgg ctcaccgtga ccggtcaggg ctcgacggtg
     4621 gtcttcgagg acgccggggc gagcatcgcg cccggtcgcg gctgcacggc cctcgcggcg
     4681 aaccgcgtgg cctgtccgcg cagctcgttc atgttcgtcc ggctcgagga cggcgacgac
     4741 gaggtcagga cgagcggcga gctcgcggac acgttcgtct tcatcgaggg cggccagggc
     4801 gccgacacga tccacggcga ggtgggggac acgagcatcg agggcggcac tgggcccgac
     4861 cggctgttcg gcggtccgct gacctccagg ctcgacgcgg tcgacgtggt gcggcgcggc
     4921 gagttcgagg acgtgcccga ccacaaccgc gagcgcgacg aggtcacgtg cgcgcagtcg
     4981 acgcgcgtgg atctgcccct gcgggtcgag gtggacgcca acgacgtcct cgccggcccc
     5041 tgcccgccgc actacctctt cctcgagacc ttcatcgtga tcgagggcac cgccggcgcc
     5101 gactcgctca cgtcggccgg gccgccgacg cacgtgaacg gcctcgccgg cgacgacacc
     5161 ctgtcgggcg agcaggacga ccggctcgac ggcggggagg gcgccgaccg cctgttcggc
     5221 cagggcctcc tcctcggcgg cagcggcgac gaccggctcg acggcgggca gagctccggc
     5281 ctgaccagcg gcgcgcggct cgacggccag tcgggcgacg acgagatcac cgggaccagg
     5341 ctgggcgact cgatcaccgg cggcagcggt cgcgacacga tctcgagcgg ccgcggccgc
     5401 gactcgatcc gtgcccgcga cggagcgcgc gatcgcatca cgtgcggacc cgaccgcgac
     5461 cgcgtgagcg ccgaccgcgg cgacaccgtc tcccgcgact gcgaggtggt ctcccgtggc
     5521 taggcggctg gcgctcctca cggccctctg cctggccgga tcgctcgccg cggcgctgcc
     5581 cgcgcaggcg agccacgggt gcagcgcatg gcgcagcaag ggggcgcacc tgatcgtcaa
     5641 gaaccgcgag gccgtggtct tcagcaaggg cgtctactac tacggctgcc tctcctcggt
     5701 cgggacgatc cggcggctgc ccgaggaggg cggcgggatc gacaccgggg gtgcggacgc
     5761 tccccagctc gccgggcgct acgtcgccta cagcacctcc ggatcgggca tcggagacga
     5821 gttcgaccgc atctacgtct acgacctccg cgtcggccgt cgcttcctga tcgagtcgag
     5881 caccttcatc cgcgacatcg cgctcaagcg caacggctcg gtcgcatgga tcgaggccgc
     5941 cccggccgac ccgggggaca cgccggtctg ggacgtgcgc gcctggagca acgaggaccg
     6001 ccagggctcg gtgctcctcg accgcgggac cgacgtcggt cccgaatcgc tggtcctgac
     6061 gcccgaccgg ctcaggatcg agtggacgcg cggcggggtc gcccgcagcg caccgttgcg
     6121 ctgaacgcgc cgaccgcgcc cctaacctct cgcgcatggc gcactcccgc ccggccgcgc
     6181 cggtgccaga ggccgacgca ctgcacgcgg cggacgctcg cctgcgtcac ctcggcgcca
     6241 tcaccgaggc cgccctcgcg cacctggacc tcgaccggct cctcgacgag ctgctgacgc
     6301 gcctgcgcgg gatcctgagc gtcgacacga tcgcgatcct gctcctcgac cccgactcga
     6361 acgagctggt cgcgcgcgcg gcgaaggggc tcgaggagga ggtcgagcag ggcgtccgga
     6421 tcccggtcgg cgccggcttc gcgggccggg tcgctgcgga ccgccggccg atctacctgc
     6481 cgaaggtcga ccgccacagc gtgatcaacc cgatcctgat caacaagggc atcgcctcga
     6541 tgctcggcgt tccgctgctg gtcgagggcg aggcgaccgg ggtgctacac gtcggatcgc
     6601 tcaccccgcg cgagttcggc gcagacgatg ccgagctgct ccagctcgcg gccgaccgca
     6661 tggcgctcgc gatcgaccac gcccgcctgt acgcgtccga gcgcgaggcg cgtgcggccg
     6721 ccgagcagcg cgccaaccag ctcacgcagc tccaggcgat caccgacgtg gcgctcggcc
     6781 ggctctcggt ggacgaggac gtgctcggcg acatgctcga gcgggtgcgc gacgtgctcg
     6841 cggtggacac cgtcgcggtc ctgctgctct cggccgaggg cgacgagctg gtcgcgcgcg
     6901 ccgcgcgcgg gctcgaggag gaggtcgagc gcggggtgcg gatcccgctc gggcgcggct
     6961 tcgcgggccg catcgccgag cagggcgagc cgctcgttct cgaggacatc gacagggcgg
     7021 acctcgtcaa cccgctcctg cgcgagaagg ggatcaagac gctcgtcggc gtcccgctcc
     7081 tggtcgagga gcgggtgatc ggcgtgctcc acgttggctc cctccacccg cgcgtgttcg
     7141 ccgaggagga gatcggcctg ctcgagcgtg ccgccgaccg gatcgcgctg gccgccgacc
     7201 gcgcccgcca gcacacgctc gcgggcctgc tccagcgcac gctgctgccc gcgcggctgc
     7261 cggaggtgcc gtcactgcgg atggccgccc gctacgtacc ggggtcggac gaggcccacg
     7321 tggggggcga ctggtacgac gtggtggagc ttccgcacgg gcgcgtcggc gtggcgatcg
     7381 gggacgtggt gagccgcggc gtgcgcgcag cggccgtgat gggccagatg cgcacggcgc
     7441 tgcgcgcgta cgcgctcgac gccgacggcg accgcccggg gccggtgctc gaccgcctcg
     7501 accggctcgt ccgcagcctc gacgagcgcg agatggccac cgtcgcctac gtcgtgatcg
     7561 accccgctga gtcggtcctc acggcgtcgc tcgccggcca cccgccgccg ctcgtgctcg
     7621 gtgcggatgg gagcacgcgg ttcctcgacg gcgtgcgctc gcgaccgatc ggcgtggtcg
     7681 ccgcgcgccg ctacgacgag acgaccgccc cactcgagcg cggcgagacg ctcatcctct
     7741 acacggacgg gctggtcgag cgccgcggcg tgcccatcga cgagggcatg cgccggctcg
     7801 aggaggtggc ggcatccgtt gccggcgccg acccggacga gctgtgcgac cggctcgtgc
     7861 ggctcgccga ggacgactcg gtggccgacg acgtcgccgt gctggccatc cgcctgcccg
     7921 ccgccgcggc ggagtcgctc gcgatgacgc ttcccgccga gccgggctcg ctctcgacca
     7981 tgcgccgtgc ggtccgggac tggctcggcc gccgcgaggt ggaccccgcc gtgggcaacg
     8041 acatcctcgt ggcgatgggc gaggccgccg ccaacgcggt ggagcacgcc tacgggccgg
     8101 gcaacgccga gttccggctg gaggcccgca tcgatgcggg cgagatcgtg gtgcggattt
     8161 acgacgaggg ccgctggcgc ccggcgcggg gatcgcaccg cgggcgcgga ttgtccatga
     8221 tggaggcgct gatggaggac gtgcaggtgg acagcgacga aagcggaacc gtggtgacca
     8281 tgcgccgcaa gctcgagggg gccggcccat gatcgcgggc tcgacacccc cgcagatcac
     8341 gatcgacgag ggagttgtga tcgcggccct gcccgacgag atcgacctcg ccaacgcgcg
     8401 cgggatcacc tcgatggtgt ccgccgccgt ccccaacgag gccgtcggcg tcgtgctcga
     8461 cctctcgggc acgacctacc tcgacagctc cggcgtccac ctgatcttcg acctcgccga
     8521 ccggctcggc gcgcgccagc agcgcctggt gctcgcggtg cccgagacct cgaacgtcag
     8581 gcgcgtgctc gagctggtgg acgtgacggt ggtggccgcg atggtccccg agctcgcccg
     8641 cgcgaaggag ctggcgcggg gctgaccgac cggtccggga ggaactgtcc gccgccgggc
     8701 gaatcccccc ggcgtgacac gcaccggcac ccaggctctc accctctcgg ctgagatccg
     8761 cgcgcgcttc gaggagttct cgcgctctca gaaggacgta ggccagtaca tcgtcgacca
     8821 cctcgacgag gccgccttcc acacggccga ggagctggcg cgcagggcca acacgtcgag
     8881 ctcgacggtc gtgcgcttcg cccaggccct cggcttcgag ggtttccccg agcttcaggc
     8941 gtccgcccgc gaggagtacc gccgggcgcg cgacagcggc ggcccggtcg gcgacccgct
     9001 cgagactcag ccgctcttcc cgatcgacca gaccgagttc gaggccgcgc tggccgccga
     9061 ccacgtcaac gtggccgaga cggcgcgcaa gctcgaccgc gacgaggtgg ccgccgccgt
     9121 ggagcggatc tcgcgctccg agcacacgct gctctgcggc accgatcaga tggcgttctt
     9181 cgcgagctac ctgcgccacc tgctgatgct gctcgacctg cgctgcgagg tcgtcgcgag
     9241 cccgagccag gagggcctcg cgcggctcgg ccggatcgac gaccgcacgc tcgtgatcgg
     9301 cttctccgcc ggtcgcccgc acccgctggt ggtccgcgcg atgaagctcg cccgccaccg
     9361 ccgcgccggc acgatcgcga tcgccgacgc caccctctcc gaggtcgcga agctcgccga
     9421 ccagcgcctc tactactcct cgaacagccc ggcgttcgtg cgctcgcaca ccgcgctgct
     9481 cgggatgatc caggcgctcg cctacggcgt gtacgcgctg gacgagtcgg cctacgccga
     9541 ccgaatcaaa gctttccggc tcaagtagcc ggctcggctc gcgcgcgggg gcggagccgc
     9601 gcgtcatgcc ccgctcgggg gtacggtcgc aggctccctc agagagcccc gatcgggaca
     9661 tgccgcgcgg ctccgacggg cgtccccggt ggccaactac tattcgcctt cgtgaaggac
     9721 accgcgacat tccgggtgaa ggcgggcctc gccgagatgc tcaagggtgg cgtgatcatg
     9781 gacgtggtca ccgccgagca ggcccgcgtg gccgaggcgg cgggcgccgt ggccgtgatg
     9841 gccctcgagc tcgtgccggc cgacatccgg gccgccggcg gggtggcccg catggccgat
     9901 cccaccaaga tcgaggagat ccagggcgcc gtcacgatcc cggtcatggc gaaggcgcgg
     9961 atcgggcact tcaccgaggc gcaggtgctg gaggccctcg aggtggacta cgtggacgag
    10021 tccgaggtgc tgaccccggc cgacgaggcc aaccacatcg acaagtggaa gttcaaggtg
    10081 ccgttcgtct gcggcgcgac caacctgggc gaggcgctgc ggcggatcgc cgagggcgcc
    10141 gcgatgatcc gcaccaaggg cgaggccggc acgggcaacg tcgtcgaggc cgtgcgccac
    10201 atgcgcacga tccgcggcga catccggcgg ctctcgtcga tggacgagtc cgagctggcg
    10261 agcgcggcga aggacctgcg cgcgccgctc gagctggtgc gcgcggtggc cgagaagtgc
    10321 ggtctgccgg tcgtgaactt ctccgccggc ggcatcgcca ccccggccga cgcggcgctg
    10381 atgatgcagc tcggcgccga gggcgtcttc gtcggctccg gcatcttcaa gtccgaggac
    10441 ccggagcgga ccgcgaaggc aatcgtcgag gccacgaccc actacgacga ccccgagcgt
    10501 gtcgctcgcg cgtcgcgcga cctcggcgcg ccgatgggcg gcatcgagat ctcctcactc
    10561 ggcgaggacg gcctgctcca gacgcgcggc tggtagccgc gtggtcgtcg gcgtactcgc
    10621 cctccagggc gatttcgagg cgcatgccgg gatgctgacg cggcttggcg cggaggcgcg
    10681 catcgtccgc acgccgggcg agctcgccgg ggtcgacgcg ctcgtggtgc ccggcggcga
    10741 gagcaccacg atcacgctgg gaatcgagcg cgaggggctc gccgagccgc tgcgcgagct
    10801 cgtgtccggc ggtacgccgg tgctggccac gtgcgccggc atgatcgtcc tcgaccgcga
    10861 tcacctcggc gtgctcgacc tcaccacgcg tcgcaacgcg ttcgggcggc aggtcatgag
    10921 cttcgaggcc gacctcgacc tacccggttt gggggagaca ccgctgcgag cggtattcat
    10981 ccgcgctccc tgggtggagg aacacggcgc gaatgtcgaa gtcttagccg aggtcgacgg
    11041 ccacccggtg gccgttcggc agggagaagt gaccgcagtg gcattccacc ccgagctgac
    11101 cagcgatgca cggctgcacc gctggctcgt cgaggccgcg ggtgcccgga tcaaggagcc
    11161 tgcatgaagg accagcgcgc cgaggcgctc gcccagatcc tcgttaggta ctcgacgaag
    11221 gtgcagaagg gcgacacagt cgtcatccag tcgacgacgc cggcggagcc cctggccctg
    11281 gcggtgtacg aggaggtgct gcgggccggc ggactgccga tggtgcagct cgcccccgac
    11341 ggggccgcgt cggcgttcta cgggctggcc tcggacgagc agctcgactg ggtctcgccc
    11401 ctctccaggt ggatcgccga ggagtccgac gtccgcatct cgctcatggc cgattcgaac
    11461 gcgcgggagc tgtcgcaggc ggacccgaag aagcaggccc gcgcgcagaa ggcgcgccgc
    11521 tcgctgctcg acacgaccat gaagcgcgcc gccagcggcg agcaccgctg ggcgctcacg
    11581 ctgtttccga ctcacgcgta cgccgccgag gccaacatgt cgctcgcgca gtacgaggac
    11641 ttctactacg cggcctgtct ggccaccgac ccggagcccg tcaccgcctg ggcgcgccag
    11701 tccgacgagg tcaagcgact cgccgagtgg atccagggca aggaggaggt ccacatcacg
    11761 gccccgggca ccgacatcaa gctcggcgtt gccgggcggc ggttcatccc gtgcaccggc
    11821 gagcacaaca tgcccgacgg cgagttcttc acgggcccga tcgaggacgc ggtcgacggg
    11881 gaggtctcgt tctcgttccc ggcgtcctac ggcggccgcg aggtgaccgg cgtgcggttc
    11941 cggttcgagg ggggcaaggt cgtcgacgcc tcggccgagc gcggcgagga ctttctgatc
    12001 gagatgctcg acaccgacga cggcgcgcgc cggctcggtg agctcggcat cgggaccaac
    12061 tacgggatcg ccgacggaac caaggagatc ctgctcgacg agaagatcgg cgggaccgtg
    12121 cacatggcga tcggcgcgag ctacccggag tcgggcggcg tgaacgagtc ggccgtgcac
    12181 tgggacatgg tctgcgacct gcgccagggt ggcgcgatcg tggtcgacgg cgtcgagctc
    12241 cagcgcgacg gccgcttcgt cgtctagctc ccgacgttca cccatccgtc gggtttccgc
    12301 ccttttgggc cgggtaccca gcggacgagg caagggagag agatgactgt cgacagggtc
    12361 aggcgcacgc ttcgcgggcg cacagaggcg cgcaacccgc gcgatccgag gcgcgccgcg
    12421 cgcacccggg tcgagaccgc gcgccgcttc ctggcccgca ccggcccggc gccgcacccg
    12481 cgc
//
LOCUS       DASVPL010000253        16434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_146843, whole genome shotgun sequence.
ACCESSION   DASVPL010000253 DASVPL010000000
VERSION     DASVPL010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16434
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146843"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1323
                     /locus_tag="VF517_13860"
     CDS             178..1323
                     /locus_tag="VF517_13860"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HEX8054072.1"
                     /translation="MAGGAHDLDWLKGALQSAIALEHSTLPLYVAAMFSLRTQSFTAY
                     NSVRSVAMEEMVHLAIACNTLSALGGAPQIRHLDPGFPSQGLPGGAEPDLSAWLAQLS
                     RPQLQAFMRIELPEFLMPDEYRHESYPTIASLYDAIDAAIDANADAVREAMKETAGSP
                     DRANQVGDNIGFKTIEYVEGEDPIPQLHAGIDEILEQGEGASSRTLHANPGSEGEESH
                     YCKFAELYYGARYEEPSPPVELTLATEPEFFRGYAIPFPDVVNTLAVPSDGYAAILAE
                     DPNAQAAEQALEAFDTAYSTVLAHLDAVWNGPAAQAFPTLGAAVGAMAGLRVPACFKV
                     MVQEVPAPAIAKLPELYPDEHERMAELTDLDRPVFYGPRFRNLNPAA"
     gene            complement(1337..2977)
                     /locus_tag="VF517_13865"
     CDS             complement(1337..2977)
                     /locus_tag="VF517_13865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX8054073.1"
                     /translation="MPDPSLVRFRRAVASACAALESRRQEVNDLNVFPVADGDTGDNM
                     AMTLRAVLAELDTMSGQDIDEIGREELVAAVARAALLGARGNSGVILSQIVRGAAEEL
                     ASRPGELIDPILVSAALARAADAAYDSVRNPAEGTMLSAVRAMAHRVAQDLAHMEPQR
                     LGPGVDPAEQDRLLAEVLERALEAGAEAVERGPEQLDVLRAAGVVDAGAYGLTVIMAG
                     VIGALRDDAPPELEHREAPARGLHHPHGHESSRFRYCTNFAVTGDGLESGPYVSKLES
                     IGDSVLVVGDPHTLKVHVHTDHPDRAMALFEGVGEVSRLDVADMRDQIAERDERLAAA
                     GEEAGCGVLAVANGPGIRRLYESLGAHVVDGGATMNPSTNDLLAGIHAAPAREVVVLT
                     NNPNVIMAAERAAELSEKPVQVVPTRSQQAGIPALLELDPDASAEQNAKALNAAVARL
                     RTGGVAPAARDDAQGRFVAGEALAYVGDELVDWGDPERALSAVIARIADGGEVLTCLA
                     GDGAPLSRATIERAVPDGVELEHHVGGQPAWWWLLCAE"
     gene            3067..3966
                     /locus_tag="VF517_13870"
     CDS             3067..3966
                     /locus_tag="VF517_13870"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX8054074.1"
                     /translation="MRTVYLGTSDFAVTVLERLADSPHRPALVVTRPDRPKGRGRKLS
                     PPPAAEAARALGIDLIQPESVNSDEARAAISAAAAGAVCICAYGALLKEPLLSEHPML
                     NVHPSLLPRWRGAAPVERAIEAGDEETGVSIMRPIAEMDAGPVCLARVEPIEDEDDYG
                     TLAPRLARLGGELLVEALDSSPPFVEQPDEGVTEAPKITGEDRRLDPGLEAGAAWRRV
                     RALNPHIGTYVELADGERLGVRRAAPTDAPPPPPAELGVDEGRLLLGCVDAALELLVV
                     QPPGGKPMSAADYLRGHGARHGA"
     gene            4015..5319
                     /gene="rsmB"
                     /locus_tag="VF517_13875"
     CDS             4015..5319
                     /gene="rsmB"
                     /locus_tag="VF517_13875"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX8054075.1"
                     /translation="MPASPARVVAQRVLERVFREGAWADRAFRGEAERAGLDPRERAF
                     AQALAYGAVQRVRTLDHVLERCSSRTLDEVQPDVRDALRLGVLQVVWLDGVPDRAAVD
                     QTVELVKAAVPRAGGFANAVMRRAAREARGIVDGLPEGTASEAALKHSHPDWLARMWW
                     KQLGADEARALMARDNEPAESALRANTLRAAPEEVRAELERAGAEARPAEPLGAGAEP
                     APVAGALPEGIVVAGAFDAHASDLFARGLVMPQSRGSMLVARTLAPQPGERVLDMCAA
                     PGAKSTHLAALMGNEGEVVAVESNEQRAGELRANAARLGATIVHVRTQDAREPVEPGG
                     FDRVLLDAPCSDLGTLQSRPDARWRKGPAAIEELAELQQELLDAALAQLRPGGRLVYS
                     TCTISPREQVGSLPGMRAEPPIQALPHRHGTDGFFIAGFTSA"
     gene            5343..5570
                     /locus_tag="VF517_13880"
     CDS             5343..5570
                     /locus_tag="VF517_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054076.1"
                     /translation="MESSSTLPRPDCPSCGEPWLSPTQLPGRFRCVYCLHRFELRSDC
                     PGCGNHATIARMTDTANVECRNCGSSMLVPI"
     gene            complement(5769..6116)
                     /locus_tag="VF517_13885"
     CDS             complement(5769..6116)
                     /locus_tag="VF517_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054077.1"
                     /translation="MKWRGILCALVALSVAVVVGVPAASGEEACQPGFVQGETFSADF
                     DADGDGFVCVNPDTGEVSDDQAAAPGTVDRNSDGIVCVRQVGNGMIVRTDNRAANPEN
                     AGCPPAFQPSPIA"
     gene            complement(6194..7468)
                     /locus_tag="VF517_13890"
     CDS             complement(6194..7468)
                     /locus_tag="VF517_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054078.1"
                     /translation="MGIGVTGLVTYAYFSLASHSLSDDDYGAITLLWSMVFIVVSVLY
                     RPVEQLLSRTIAERDAHGRGGSEHLRVAATIQLALGFVFAAVALALRGPIEDDLFEGS
                     ATLYWVLIAAVLAYAASYFARGFLAGNRRFGLYGLLVLLEATSRVMFALAVAVGIADG
                     QSVVAFGILAAPLFSLAVVPWALRLRLRADAEVGEPVPAEGPAGESTKPAEPEFTLAH
                     GTGFAAAVLLIMLAEQTFLNAGPLLVKATGGAEGAALAGFAFNVLLIARAPLQLFQSV
                     QTSILPHLTHVSATGDRAAFSRAVTTTLKAIAAFAGLVAVGMLVLGPWAMELFFGESD
                     EYDRGGLALVAVGMGLYLAAATLNQAALARGLTKQAALCWVGSALTFVLMLLLPGFSS
                     PVLQLELAYVAGALLLCALLFALYRRGSIPPA"
     gene            complement(7629..8243)
                     /locus_tag="VF517_13895"
     CDS             complement(7629..8243)
                     /locus_tag="VF517_13895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054079.1"
                     /translation="MSATPTVPTGNTTDKYNTTNPVMRRMMDGFERDLNELFAQAAPR
                     SILDVGCGEGVLTSEWADRLDGGRIVGIDLDDPKLRAEWEKRQRANLEYRVEEATSLS
                     FEDDEFDVATAIEVLEHVPEPEATLAEMARVAQRHLLVSVPREPIWRMLNMARGAYLG
                     ALGNTPGHLNHWTKVGFVSLLSRYGTVAEVRTPLPWTMALVSVS"
     gene            complement(8240..9298)
                     /locus_tag="VF517_13900"
     CDS             complement(8240..9298)
                     /locus_tag="VF517_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8054080.1"
                     /translation="MKLIIQIPCLNEEDQLPTTLADLPRQVEGFDEVEWLIVDDGSTD
                     RTIEVAKANGVDHIVRLTNNKGLATGFQAGLDACLKLGADVIVNTDADNQYSAADIPK
                     LVAPIVEGRADMVVGDRVVESIEHFSPLKKTLQRLGSWVVRRASETTVPDTTSGFRAY
                     NREAAIQVQVVSKYTYTLETIIQAGKMLVAIDHTPIRTNAKTRESRLFKSMGAYVRRN
                     TVSIFRIYSMYEPLRVFMTAAAIVGFAALVIWIRFAYFYVVEDEGAGHVQSLILGAVL
                     FIAAVQLAALGIVGDILAGSRILQQRILERVRRVELQLGVEPSHYEPGAGGERPRTTG
                     AEAGRATGNTEEREALKL"
     gene            9343..9735
                     /locus_tag="VF517_13905"
     CDS             9343..9735
                     /locus_tag="VF517_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054081.1"
                     /translation="MTQRLAAAALCASFALAGCGDGADQNERQRAQEVVEKFALAEGP
                     EACDYLGGQALEDNYGGRDYATGRERCEKAAKRFEGEAITVKAVKVTSETTARVDAES
                     KAGRLYVVSLSKPADDWVIERIIQQRRQ"
     gene            complement(9784..9999)
                     /locus_tag="VF517_13910"
     CDS             complement(9784..9999)
                     /locus_tag="VF517_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054082.1"
                     /translation="MSDQTGDQPAKRGDAAYRAQKDAINARNDAARKAGREYRQNEDR
                     KAAVRRAEADRADLADLRDTFGGNTAH"
     gene            complement(10086..10421)
                     /locus_tag="VF517_13915"
     CDS             complement(10086..10421)
                     /locus_tag="VF517_13915"
                     /inference="COORDINATES: protein motif:HMM:NF007494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX8054083.1"
                     /translation="MTIAFEDFEKVDVRVGVVTDVEDFPRARNPSLKLTIDFGPEIGV
                     KRSSAQVTNYAHDELRGMQVLAVVNFEPRNIAGFMSEVLVLGVESAAGDGIALLTPSR
                     DAVVGSRMY"
     gene            complement(10418..12217)
                     /locus_tag="VF517_13920"
     CDS             complement(10418..12217)
                     /locus_tag="VF517_13920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8054084.1"
                     /translation="MRARLRRPTGHELIAYGLLLAIVVSGLYLRLRNNGYGLPYVYNY
                     DEAQHFTNKAVTMINGDLDPGYYQNPSAFTYLVFLMLKVLYGVFGLELAEGSIRRQFT
                     QDPTQIWELARGLAAVLAMAGVVATFWVGKRIWGLRVAVVAAFVLCFAFLPVVYSRIA
                     LTDTGTFLPTVLCVYFSLRAWEDGQRRHYVLAGVAAGFAVGFKYTAGLVLVPLFVAAL
                     IRFRRDRETPLVRRSDLHGLVFAGIATLVAFAITTPFFFVKPISALYQLKQQAEAAGD
                     SAKLGQSQEGGFSYYVKSLTWGFGWAAIAAAIAGAVIEIRRDRWRGILLALFPLVLFL
                     YMGSQTRYFGRWLLPMYPVLALLAAVGLVQLASLIRRRPVVQGLVLAALAAGIMAQSI
                     VADVRTSEVLGKEDTRQIARDWLERTQPEAIRIVIEPGVPDTYYRVVGSRNRQRQFVR
                     GFIRDVRRRVVLDAPEGADTTYAATLTPDLIDLYRQQGFCLVMTMSVIRGRAENAKVP
                     QALAYYDRLERESKRVFYASPYDPGAEKVPLHYDFSYNYYPTAFARPGPEVGIYRLND
                     CVEKTDSVPVGPLGTKGLDKGVATSYRPVDPQQ"
     gene            12300..13424
                     /gene="ribD"
                     /locus_tag="VF517_13925"
     CDS             12300..13424
                     /gene="ribD"
                     /locus_tag="VF517_13925"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX8054085.1"
                     /translation="MAETPAGERDARHLRRAVELAAEARGMTSPNPLVGAVIVKSGRV
                     IGEGFHSAAGRPHAEREALAACAEDPAGATMYVSLEPCAHEGRTPPCTDAIVEAGIAR
                     VVVASDDPTEKANGRGLGILRDEGVTVDVLNGEVAASARLLNQPFRKHARTGRPLVVM
                     KCAMTLDGKVATSTGDSQWISGEESRARAHRWRAELDAVVVGIGTALSDDPLLTARVE
                     AVARQPSRVVFDSEARLPLASQLVRTVDQAPLIVVCGRAAPRTATEALEAAGAEVVVV
                     SGENEGARVGAALDELGGRGIQSLLLEGGPHLTGAFLDAGEIDVVHAFVAPMLAGGRN
                     AKPPVEGEGIERIAAGRRALSSDVERIGDDVLISARLAEW"
     gene            13424..13999
                     /locus_tag="VF517_13930"
     CDS             13424..13999
                     /locus_tag="VF517_13930"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX8054086.1"
                     /translation="MFTGLVQQKGTVASVEGGRLSVRTESPLDLEEGASVSVNGVCLT
                     ATAIAAGGFDADVMPETLRRTALGTLAAGHPVNLELALRASDRLGGHMVQGHVDATGT
                     VEALRDEGFARVVRIAAGTDVLRYVVEKGSIAVDGVSLTVSAVGDDWFEVSLIPETLA
                     RTTLGGAEPGRVVNLEVDVLAKYVEKLVSAR"
     gene            13996..15318
                     /locus_tag="VF517_13935"
     CDS             13996..15318
                     /locus_tag="VF517_13935"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX8054087.1"
                     /translation="MSSAETRAPFSTIEEAIEDIRAGRMVVVCDAEDRENEGDLTLAA
                     QFATPDAINFMAKEGRGLICLALTPDRCDELGLDLMAAKNESPYETAFTVSVEAREGV
                     TTGISAHDRAHTIQVAIDPRTQPRDLVQPGHVFPLKAKAGGVLERTGQTEAAVDLARL
                     AGLTPAGVICEIMNDDGTMARVPDLVDYCARHDLKMVTVADLIAYRRRHDKLVERVVE
                     TRLPTDFGDFSAVGFRSLVDGKHHIAMVKGDVDGVEDVLVRVHSECLTGDVFHSQRCD
                     CGLQLETALARIDSEGCGVLLYLAQEGRGIGLLNKLKAYKLQEEGLDTVDANLELGLP
                     ADLRDYGIGAQILVDLGLTTIRLLTNNPKKIVGLEGYGLKVSDQLPIAMPAGAHNRDY
                     LRAKRDKLGHLLHHQGLALDEEMIHEEQVHDRERSDGAPRVPRPDPPE"
     gene            15315..15725
                     /gene="ribH"
                     /locus_tag="VF517_13940"
     CDS             15315..15725
                     /gene="ribH"
                     /locus_tag="VF517_13940"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX8054088.1"
                     /translation="MSAWAIVVGRFYEDLADELVAGAKRVFEGEVDVYDVPGAFELPL
                     GALYCARSGRYSGVACLGAVIRGETSHYDHVCEEAAAGIARVSLDTGVPCSFGVLTVE
                     NMDQARARLGDKRHQGEDAARAVVAMAAMREALA"
     gene            15722..>16434
                     /locus_tag="VF517_13945"
     CDS             15722..>16434
                     /locus_tag="VF517_13945"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEX8054089.1"
                     /translation="MSELVNLWSDTQTRPSAGMRRAMAEAEVGDEQRGADPTVNALQE
                     RVAELLGHEAALFLPSGTMCNQIGIRLHVRPGEAMILHGSAHPIIAEAGGPAAHAGAM
                     IVAVEGEGGMFDGAAVRGSLSVPGNRYEARSALVNVEQTTNMGGGRVWPLELMRDVVS
                     AARAAGLRAHLDGARLMNAVVAAGVPAADYASQFDTAWIDFTKGLGAPVGACLAGSRE
                     LIEEAWRLKQMMGGALRQAG"
BASE COUNT         2348 a         5869 c         6029 g         2188 t
ORIGIN      
        1 catcttggcg cgcggctgct gcgggttgac gtcggtcagg cgggacatgg tctgcccttc
       61 ttcagggagc ggggcggggg cggcgcgact ctaatcgcgc cgcgtccgcg aagtcaaatg
      121 ccgctgatcg atcagcgcaa gttgcacatg tgcccggccc gcgggtaggg tcggccggtg
      181 gccggcggag cccacgacct cgactggctg aagggcgcgc tccagtccgc gatcgccctc
      241 gagcactcga cgctgccgct ctacgtcgcg gccatgttct cgcttcggac gcagagcttc
      301 accgcgtaca actccgtgcg cagcgtggcg atggaggaga tggtccacct cgcgattgcg
      361 tgcaacacgc tctccgcgct ggggggcgct ccgcagatcc gccacctcga ccccggcttc
      421 ccctcgcagg ggctcccggg cggcgccgag ccggacctga gcgcgtggct cgcgcagctc
      481 tcgcgccccc agctccaggc cttcatgcgc atcgagctcc cggagttcct gatgccggac
      541 gagtaccgcc acgagtccta cccgacgatc gccagcctct acgacgcgat cgacgccgcg
      601 atcgacgcga acgccgacgc ggtgcgcgag gcgatgaagg agacggccgg ctcgccggac
      661 cgtgcgaacc aggtgggcga caacatcggg ttcaagacca tcgagtacgt cgagggcgag
      721 gacccgatcc cccagctcca cgccgggatc gacgagatcc tcgagcaggg cgagggagcg
      781 tcgagccgga cgctgcacgc gaaccccggc tccgagggcg aggagtcgca ctactgcaag
      841 ttcgcggagc tctactacgg cgcgcgctac gaggagccga gcccgcccgt cgagctgacc
      901 ctcgcgaccg agcccgagtt cttcaggggc tatgcgatcc cgttcccgga cgtggtcaac
      961 accctcgccg tgccgtccga cggctacgcg gcgatcctcg ccgaggaccc gaacgcgcag
     1021 gccgccgagc aggcgctcga ggcgttcgac acggcctact cgaccgtcct cgcccatctc
     1081 gacgccgtct ggaacggacc cgccgcgcag gcgttcccga cgctgggcgc ggcggtcggc
     1141 gcgatggccg ggctccgcgt gcccgcctgc ttcaaggtca tggtccagga ggttcccgcc
     1201 ccggcgatcg cgaagctgcc cgagctctat ccggacgagc acgagcggat ggcggagctg
     1261 accgacctcg accggcccgt gttctacggg ccgcgcttcc gcaacctgaa ccccgcggcc
     1321 tagcgccgcg cccgcgctac tcggcgcaga gcagccacca ccaggcgggc tggccgccca
     1381 cgtggtgctc gagctcgacg ccgtcgggga cggcgcgctc gatcgtggcg cggctgagcg
     1441 gcgcgccgtc gcccgcgagg cacgtgagca cctcgccgcc gtcggcgatc cgggcgatga
     1501 ccgcgctgag cgcgcgctcc gggtcgcccc aatcgaccag ctcgtcgccg acgtaggcga
     1561 gcgcctcgcc cgccacgaag cggccctgcg cgtcgtcgcg agcggccggg gccacgccgc
     1621 cggtgcgcag gcgcgcgacc gcggcgttca gcgccttcgc gttctgctcg gcgctcgcgt
     1681 ccgggtcgag ctcgagcagc gccgggatgc cggcctgctg cgagcgggtc ggcacgacct
     1741 gcacgggctt ctccgagagc tcggccgcgc gctcggccgc catgatcacg ttcgggttgt
     1801 tcgtcagcac cacgacctcg cgcgcggggg ccgcgtggat gccggcgagc aggtcgttgg
     1861 tggaagggtt catggtcgcc ccgccgtcga ccacgtgcgc gccgaggctc tcgtacagcc
     1921 gccggatgcc cggtccgttg gcgaccgcga gcacgccgca gcccgcctcc tcgcccgcgg
     1981 cggccaggcg ctcgtcgcgc tcggcgatct gatcgcgcat gtcggccacg tcgaggcgcg
     2041 agacctcgcc cacgccctcg aagagggcca tggcgcggtc gggatggtcg gtgtgcacgt
     2101 gcaccttgag cgtgtgcggg tcgccgacga ccagcaccga gtcgccgatc gactcgagct
     2161 tcgacacgta cggccccgac tcgaggccgt cgccggtcac ggcgaagttg gtgcagtagc
     2221 ggaagcggct cgactcgtgg ccgtgcgggt ggtggagccc gcgtgccggt gcctcgcgat
     2281 gctcgagctc gggcggcgcg tcgtcgcgca gagcgccgat cacaccggcc atgatcacgg
     2341 tgaggccgta ggcgccggcg tccaccacgc cggcggcgcg caggacgtcg agctgctcgg
     2401 ggccgcgctc gaccgcctcg gcaccggcct cgagggcgcg ctccagcacc tccgccagca
     2461 gccggtcctg ctcggcgggg tcaacgcctg gcccgagccg ctgaggctcc atgtgtgcga
     2521 ggtcctgcgc cactcggtgc gccatcgcgc ggacggccga gagcatcgtc ccctcggcgg
     2581 ggttgcggac cgagtcgtac gcggcgtcgg ccgcgcgggc gagagcggcg ctcacgagga
     2641 tcggatcgat cagctcgccg ggccgcgacg ccagctcctc cgcggcgccg cgcacgatct
     2701 gcgagaggat cacgccgctg ttgccgcgcg cgccgagcag cgcggcgcgg gccacggcgg
     2761 cgaccagctc ctcgcggccg atctcgtcga tgtcctggcc gctcatcgtg tcgagctcgg
     2821 cgagcaccgc gcgcagcgtc atcgccatgt tgtcgccggt gtcgccgtcg gcgaccggga
     2881 acacgttgag gtcgttgacc tcctgccgcc ggctctcgag cgccgcgcag gcgctcgcga
     2941 cagctcgccg gaagcgaacc aggctgggat ctggcatggc catgtactac acccacacta
     3001 cgccgcgttt cgggcgcatg gccgcgcggc gcgcccgcga ccggagattg cggcagtctg
     3061 tgcgccgtgc ggaccgtcta cctcggcacc tcggacttcg ccgtcaccgt gctcgagcgg
     3121 ctggcggatt cgccgcaccg gccggcgctc gtggtcacgc ggccggaccg gccgaagggc
     3181 cgcgggcgca aactctcgcc gccgcccgca gccgaggcgg cgcgcgccct cggcatcgac
     3241 ctcatccagc ccgagagcgt gaactcggac gaggcccggg cggcgatctc cgcggccgcg
     3301 gccggcgcgg tctgcatctg cgcctacggc gcgctgctga aggagccgct gctgtcggag
     3361 cacccgatgc tgaacgtcca cccctcgctg ctgccgcgct ggcgcggcgc ggcaccggtc
     3421 gagcgcgcga tcgaggccgg ggacgaggag accggggtct cgatcatgcg gccgatcgcc
     3481 gagatggacg cggggcccgt ttgcctggcg cgggtggagc cgatcgagga cgaggacgac
     3541 tacgggacgc tcgcaccgcg gctcgcacgg ctcgggggcg agctgctcgt ggaggcgctc
     3601 gacagctcgc cgccgttcgt ggagcagccg gacgagggcg tcacggaggc gccgaagatc
     3661 acgggcgagg accgccggct cgatccgggc ctcgaggcgg gcgcggcgtg gcggcgcgtg
     3721 cgcgctctga acccgcacat cggcacctac gtggagctcg ccgacggcga gcgcctgggc
     3781 gtgcggcgtg cggcgccgac ggacgcgccc cctcccccgc ccgcggagct gggcgtggac
     3841 gaggggcggc tgctgctcgg ctgcgtcgac gccgcgctcg agctgctcgt ggtgcagccg
     3901 cccggcggga agccgatgag cgcggccgac tacctgcgcg gtcacggcgc gcggcacggc
     3961 gcttgacggc gcggcgcggc ggccggtgcc ggtgacgcgg cgcgggagcc gctgatgccg
     4021 gcttcgccgg cgcgcgtcgt cgcccagcgt gtgctcgaac gcgtcttccg ggagggcgcc
     4081 tgggccgacc gtgccttccg cggcgaggcc gagcgagccg gcctcgaccc gcgcgagcgc
     4141 gccttcgcgc aggcactcgc gtacggcgcc gtccagcgcg tgcgcacgct cgaccacgtg
     4201 ctcgaacgtt gctcgagccg cacgctcgac gaggtccagc cggacgtccg cgacgcgctg
     4261 cggctcgggg tgctccaggt cgtctggctc gacggcgtgc cggaccgcgc ggcggtggac
     4321 cagaccgtcg agctggtgaa ggccgccgtg ccgcgcgcgg gcgggttcgc caacgcggtc
     4381 atgcggcggg cggctcgcga ggcgcgcggc atcgtggacg ggctgcccga ggggaccgcg
     4441 tcggaggccg cgctcaagca ctcgcacccc gactggctcg cccggatgtg gtggaagcag
     4501 ctcggtgcgg acgaggcgcg cgcgctgatg gcgcgcgaca acgagccggc cgagagcgcg
     4561 ctccgggcga acacgctccg cgcggcgccc gaggaggtcc gcgcggagct ggagcgcgcg
     4621 ggcgccgagg cgcggccggc ggagccgctc ggcgcgggcg cggagccggc ccccgtcgcg
     4681 ggcgccctcc ccgagggcat cgtcgtcgcc ggcgccttcg acgcgcacgc ctccgacctc
     4741 ttcgcgcgcg gcctcgtgat gccgcagtcg cgcggctcga tgctcgtggc gcgcaccctc
     4801 gccccgcagc cgggcgagcg cgtgctcgac atgtgcgcgg ctcccggcgc gaagagcacg
     4861 cacctggcgg ccctgatggg gaacgagggc gaggtcgtcg ccgtggagtc gaacgagcag
     4921 cgcgccggcg agctacgcgc gaacgccgcc cggctcggcg cgacgatcgt ccacgtgcgc
     4981 acgcaggacg cgcgcgagcc ggtcgagccc ggcggcttcg accgcgtcct gctggacgcc
     5041 ccgtgctcgg acctcggcac gctccagtcg cgccccgacg cgcgctggcg caaggggccg
     5101 gcggcgatcg aggagctggc ggagctccag caggagctgc tcgacgcggc gctcgcccag
     5161 ctcaggcccg gcggcaggct cgtctactcc acctgcacga tcagcccgcg cgagcaggtg
     5221 ggctccctgc ccgggatgcg cgcggagcct ccgattcagg cgctccctca ccgtcacggc
     5281 acggacggat tcttcatcgc cgggtttacc tccgcctgac cacggtgtag aagaacgctc
     5341 cgatggagtc ctcgagcacc ctcccccgcc ccgactgccc ctcctgcggc gagccgtggc
     5401 tcagcccgac ccagctcccg ggacgcttcc gctgcgtcta ctgcctgcac cgcttcgagc
     5461 tgcgctccga ctgccccggc tgcggcaacc acgcgacgat cgcgcgcatg accgacaccg
     5521 cgaacgtcga gtgccgcaac tgcggcagca gcatgctggt cccgatctag acggggtccg
     5581 ggagcgaggg tgtgcaagag gccgcttggt gctgagcacg gccctaccgc ccgacatctc
     5641 gtcggtgccg ctgcccgagg aatcgcaaga cgaggcgtcc gtcgaggacg tcgtcgagcg
     5701 catcgaccag tcgatctacg ccgcgctggt cgccagctgg cgaccagcgc gccggccggc
     5761 cgtcgtcgct atgcgattgg cgacggctgg aacgccggcg ggcagcccgc gttctccggg
     5821 ttggcggccc ggttgtcggt cctgacgatc atcccgttcc cgacctggcg gacgcacacg
     5881 atgccgtcgc tgttgcggtc gacggtcccg ggggccgcgg cctgatcgtc ggagacctcg
     5941 ccggtgtccg ggttcacgca cacgaacccg tccccgtccg cgtcgaaatc cgcggagaac
     6001 gtctcaccct ggacgaagcc cggctggcag gcctcctcgc cactcgccgc cggcacaccc
     6061 acgacaaccg ctacggacag cgccaccagg gcgcagagga tcccccgcca cttcatagcc
     6121 agttcccttc ctcgagcccc cctggcggag cccgggccag aacctcgaac gtaacccggc
     6181 tacccccgga cggtcaagcg ggagggattg atccgcgcct atagagcgcg aagaggagcg
     6241 cgcacagcag cagcgccccc gccacgtacg cgagctcgag ttggaggacc gggctgctga
     6301 accccggcag cagcagcatg agcacgaacg tgagcgccga gccgacccaa cagagcgccg
     6361 cctgcttcgt gagcccgcgc gcgagcgccg cctggttgag cgtggcggcc gccaggtaga
     6421 gccccatacc cacggccacg agcgcgaggc cgccgcggtc gtactcgtcc gactccccga
     6481 agaacagctc catcgcccac gggccgagga cgagcatccc cacggccacg agtcccgcga
     6541 aggccgcgat cgccttcagg gtcgtggtca ccgcccggct gaaggcggcc cggtcgccgg
     6601 tcgcgctcac gtgcgtgagg tggggcagga tcgaggtctg caccgactgg aagagctgga
     6661 gcggagcgcg ggcgatcagc agcacgttga acgcgaagcc cgcgagcgcg gcgccctccg
     6721 cgcccccggt cgccttcacg agcagcggcc cggcgttcag gaaggtctgc tcggcgagca
     6781 tgatcagcag caccgccgcg gcgaagccgg tcccgtgcgc gagcgtgaac tcgggctccg
     6841 ccggtttcgt ggactcgccg gcgggcccct cggccggcac gggctcaccc acctcggcat
     6901 ccgcgcgcag gcgcagcctg agggcccacg gcaccacggc gagcgagaac agcggcgccg
     6961 ccaggatgcc gaacgcaacc accgactgcc cgtccgcgat ccccaccgcc acggcgagcg
     7021 cgaacatcac ccgcgaggtg gcctcgagca gcaccagcag cccgtagagc ccgaagcggc
     7081 ggttgccggc gaggaacccg cgggcgaagt agctcgccgc gtacgccagc accgcggcga
     7141 tcagcaccca gtagagcgtc gcggagccct cgaacaggtc gtcctcgatc ggcccgcgca
     7201 gcgccagcgc cacggcggcg aaaacgaacc cgagcgcgag ctggatcgtc gccgccacgc
     7261 gcaggtgctc gctgccgccg cggccgtggg cgtcgcgctc ggcgatcgtg cgcgagagga
     7321 gctgctccac cgggcggtag agcaccgaca cgacgatgaa gaccatcgac cacagcagcg
     7381 tgatcgcgcc gtagtcgtcg tcgctcagcg aatggctggc tagcgagaag tacgcgtagg
     7441 tgacgagtcc ggtgaccccg atccccaccg acaggatcgc ggcgccgcgg ccgtagccgc
     7501 cggcctcagt cgaggaccgt gagcgttctc gtggccgcga aaaccgagtc aatcaaggac
     7561 gcagggtcga aggcttggtt ggccaagccg agacccgagg acgccgagtg acgacggttt
     7621 gcagcggatc acgagacgct cacaagcgcc attgtccacg gcaaaggcgt gcgcacctcg
     7681 gcgaccgtcc cgtagcgcga cagcagcgac acgaacccga ccttggtcca gtggttcagg
     7741 tggccgggcg tgttccccag ggcaccaagg taagcgccgc gggccatgtt gagcatgcgc
     7801 cagatcggct cgcgtgggac cgagacgagc aggtggcgct gcgccacccg cgccatctcg
     7861 gccagcgtgg cctcgggctc gggcacgtgc tcgagcacct cgatcgcggt ggccacgtcg
     7921 aactcgtcgt cctcgaacga gagggaggtc gcctcctcga cgcggtactc gaggttcgcc
     7981 cgctggcgct tctcccactc ggcgcggagc ttggggtcgt cgaggtcgat cccaacgatc
     8041 cgcccgccgt cgaggcggtc cgcccactcc gaggtgagca cgccctcgcc gcagccgacg
     8101 tcgaggatcg acctcggcgc cgcctgcgcg aacagctcgt tcaggtcgcg ctcgaacccg
     8161 tccatcatcc gccgcatgac ggggttcgtg gtgttgtact tgtcggtggt gttgccggtc
     8221 gggacggtcg gcgtggcgct cacagcttca gcgcctcccg ctcctccgtg ttgccggtcg
     8281 cgcggccggc ctcggcgccg gtggtgcgcg ggcgctcgcc gccggccccc ggctcgtagt
     8341 gcgacggctc gacgccgagc tggagctcca cgcggcgcac gcgctcgagg atccgctgct
     8401 ggaggatccg gctgccggcc aggatgtcgc cgacgatgcc gagcgccgcg agctgcaccg
     8461 ccgcgatgaa cagcaccgcg ccgaggatca gcgactgcac gtgcccggcg ccctcgtcct
     8521 cgaccacgta gaagtaggcg aaccgaatcc agatcacgag cgcggcgaaa ccgacgatcg
     8581 cagcggctgt catgaacacc cgcagcggct cgtacatcga gtagatccgg aagatcgaga
     8641 cggtgttgcg gcgcacgtac gcgcccatcg acttgaacag acgcgactcg cgggtcttcg
     8701 cgttcgtgcg gatcggcgtg tggtcgatcg ccacgagcat cttgcccgcc tggatgatcg
     8761 tctcgagcgt gtaggtgtac ttcgagacga cctgcacctg gatcgcggcc tcgcggttgt
     8821 acgcgcggaa gccggacgtg gtgtcgggca cggtcgtctc cgaggcgcgg cgcacgaccc
     8881 agctacctag ccgctgcagc gtcttcttca gcggcgagaa gtgctcgatc gactcgacca
     8941 cccggtcgcc caccaccatg tccgcgcggc cctcgacgat cggcgcgacg agcttcggga
     9001 tgtcggccgc ggagtactgg ttgtcggcgt cggtgttgac gatcacgtcg gcgccgagct
     9061 tcaggcacgc gtcgaggccc gcctggaacc ccgtggcgag gcccttgttg ttcgtcaggc
     9121 ggacgatgtg gtcgacgccg ttcgccttcg cgacctcgat ggtgcggtcg gtcgagccgt
     9181 cgtcgacgat cagccattcc acctcgtcga agccctcaac ctgacgggga aggtccgcga
     9241 gtgtggtcgg aagctgatcc tcctcgttga ggcaggggat ctggatgatg agcttcatgc
     9301 cggcgcgtcc agagggtatc ggccgcgggt aacgtaatcg ccgtgacgca acgcctcgcc
     9361 gcagccgccc tctgcgcgtc cttcgccctc gccggttgcg gcgacggcgc cgaccagaac
     9421 gagcgtcagc gcgctcagga ggtcgtcgag aagttcgcgc tcgccgaggg gccggaggcg
     9481 tgcgactacc tcggcggcca ggccctcgag gacaactacg gcggccgcga ctacgccacc
     9541 ggcagggagc gctgcgagaa ggccgccaag cgcttcgagg gcgaggcgat caccgtcaag
     9601 gccgtgaagg tcacgagcga gacgaccgcg cgcgtcgacg cggagtcgaa ggccgggcgg
     9661 ctctacgtcg tgtcgctgtc gaagcccgct gacgactggg tgatcgagcg gatcatccag
     9721 cagcgcaggc agtagaccgc ggggggcgcc gcgccccacg ccgcggccgg gcggtgcccg
     9781 cgcctagtgc gcggtgttgc cgccgaaggt gtcgcgcagg tcggccaggt cggcgcggtc
     9841 ggcctcggcg cggcgcaccg ctgccttgcg atcctcgttc tggcggtact cgcgcccggc
     9901 cttgcgggcg gcgtcgttgc gcgcgttgat cgcgtccttc tgggcccggt aggcggcgtc
     9961 gccgcgcttg gcgggctggt cgccggtctg atcactcatg ggtctccccc ggttgctgtt
    10021 gaccgaggtc tccctcagtg gcttcatgga aagcataggg cccgtgccgg gcgcttgtgt
    10081 cggagctagt acatgcgcga gccgacgacc gcatcgcggc tcggcgtgag cagcgcgatc
    10141 ccgtcgccgg ccgcggactc cacgccgagc accagcacct cgctcatgaa ccccgcgatg
    10201 ttgcgcggct cgaagttcac gacggccagc acctgcatgc cgcgcagctc gtcgtgggcg
    10261 tagttggtga cctgagcgct cgagcgcttc acgccgatct cggggccgaa gtcgatggtc
    10321 agcttgagcg acgggttgcg cgcccgcggg aagtcctcca cgtcggtgac cacgcccacc
    10381 cggacgtcga ccttctcgaa gtcctcgaac gcgatcgtca ctgctgcggg tcgaccggcc
    10441 ggtagctcgt ggccacgccc ttgtcgaggc ccttcgtgcc gagcggcccc accggcaccg
    10501 agtcggtctt ctcgacgcag tcgttcaggc ggtagatccc tacctcgggg cccggccgcg
    10561 cgaacgcggt cgggtagtag ttgtacgaga agtcgtagtg cagggggacc ttctcggcac
    10621 ccgggtcgta cgggctcgcg tagaagacgc gcttcgactc gcgctcgagc cggtcgtagt
    10681 acgcgagcgc ctgcgggacc ttcgcgttct cggcgcgccc acggatcacg ctcatagtca
    10741 tcacgaggca gaagccctgc tggcggtaga ggtcgatcag gtcgggtgtg agcgtggccg
    10801 cgtaggtggt gtcggctccc tcgggggcgt cgagcaccac gcgccggcgc acgtcgcgga
    10861 tgaagccgcg cacgaactgg cgctggcggt tgcggctgcc gaccacgcgg tagtaggtgt
    10921 cgggcacgcc gggctcgatc acgatcctga tcgcctcggg ctgggtgcgc tcgagccagt
    10981 cgcgcgcgat ctgccgcgtg tcctccttgc cgagcacctc cgaggtgcgc acgtccgcga
    11041 cgatcgactg ggccatgatc cccgcggcga gcgcggcgag cacgaggccc tgcaccaccg
    11101 ggcggcgccg gatcagcgag gcgagctgca cgagcccgac cgccgcgagc agcgccagca
    11161 ccgggtacat cgggagcagc cagcgcccga agtagcgcgt ctgcgacccc atgtagagga
    11221 acaggacgag cgggaacagc gccagcagga tgccgcgcca gcggtcgcgg cggatctcga
    11281 tgaccgcgcc cgctatagcg gcggcgatcg ccgcccagcc gaagccccag gtcaggctct
    11341 tcacgtagta gctgaagccg ccctcctgcg actggccgag cttggcggag tcgcccgccg
    11401 cctccgcctg ctgcttgagc tggtagagcg cgctgatcgg cttgacgaag aagaacggcg
    11461 tggtgatcgc gaacgcgacc agcgttgcga tgcccgcgaa cacgagcccg tggaggtcgc
    11521 tccgccggac gagcggcgtc tcccgatcgc ggcgaaagcg gatcagcgcg gccacgaaca
    11581 gcggcacgag cacgagcccg gccgtgtact tgaagccgac cgcgaagccg gccgccacgc
    11641 ccgcgagcac gtagtggcgg cgctgcccgt cctcccaggc gcgcagcgag aagtagacgc
    11701 agaggaccgt cggcaggaac gtgccggtgt cggtcagcgc tatccgcgag tacacgaccg
    11761 gcaggaacgc gaagcacagg acgaacgccg ccaccaccgc cacgcgcagg ccccagatcc
    11821 gcttcccgac ccagaacgtg gccaccacgc cggccatcgc gaggacggcg gcgagcccgc
    11881 gcgcgagctc ccagatctgc gtcgggtcct gcgtgaactg ccgccggatg ctgccctcgg
    11941 cgagctccag gccgaacacg ccgtagagca ccttcagcat caggaagacc aggtatgtga
    12001 aggccgacgg gttctggtag tagcccgggt cgagatcgcc gttgatcatc gtcacggcct
    12061 tgttcgtgaa gtgctgcgcc tcgtcgtagt tgtagacgta cgggagcccg tagccgttgt
    12121 tgcgcaggcg caggtagagg ccgctgacga cgatggccag aagcaggccg taagcgatca
    12181 gctcatggcc ggtggggcgc cgcaggcggg ctcgcatccg gccgcgcagg ttagcggcgg
    12241 gcccctgtgc cattggtaag gttgactcgc gagtcaacta cgacgaggcg gactggaaat
    12301 tggctgagac ccccgctggc gagcgggatg cgcgccacct gagacgggcc gtcgagctgg
    12361 ccgcagaggc gcgcgggatg accagcccga accccctcgt cggcgcggtg atcgtgaagt
    12421 ccggccgcgt gatcggcgag ggcttccaca gcgccgcggg ccgcccccac gccgagcgcg
    12481 aggcgctcgc cgcctgcgcc gaggaccccg ccggcgcgac catgtacgtg tcgctcgagc
    12541 cgtgcgcgca cgagggccgc acgccgccgt gcaccgacgc gatcgtcgag gccggcatcg
    12601 cgcgcgtggt ggtcgcatcc gacgatccca ccgagaaggc gaacggccgc gggctcggga
    12661 tcctgcgcga cgagggcgtg accgtcgacg tgctgaacgg cgaggtggcg gcgagcgcgc
    12721 ggctcctcaa ccagccgttt cgcaagcacg cgcgcaccgg ccggccgctg gtggtcatga
    12781 agtgcgcgat gacgctcgac ggcaaggtcg cgaccagcac gggcgactcg cagtggatct
    12841 cgggcgagga gagccgcgcc cgcgcgcacc gctggcgtgc cgagctcgac gcggtggtgg
    12901 tcgggatcgg gaccgctctc tccgacgacc ccctcctcac cgcgcgcgtg gaggccgtcg
    12961 cgcgccagcc gagccgcgtc gtgttcgact ccgaggcgcg gctgccgctc gccagccagc
    13021 tggtgcgaac cgtggaccag gctccgctga tcgtcgtctg cggccgggcg gcgccgcgca
    13081 ccgccaccga ggcgctggag gcggccggcg ccgaggtggt cgtggtctcc ggcgagaacg
    13141 agggcgcacg cgtgggcgcc gcgctcgacg agctcggcgg gcgcgggatc cagtccctcc
    13201 ttctcgaggg cgggccgcac ctcaccggag ccttcctcga cgcgggggag atcgacgtgg
    13261 tgcacgcgtt cgtcgcgccg atgctcgccg gcgggcgcaa cgccaagccg ccggtcgagg
    13321 gcgaggggat cgagcggatc gcggccgggc gccgcgcgct gtcgagcgac gtcgagcgga
    13381 tcggcgacga cgtgctgata tcggctcgcc tcgcggagtg gtgatgttca cgggcctcgt
    13441 acagcagaag ggcacggtcg catccgtgga gggcgggcgc ctctcggtcc gcaccgagtc
    13501 cccgctcgac ctcgaggagg gggcatcggt gtcggtcaac ggcgtgtgtc tcaccgcgac
    13561 cgccatcgcc gccggcggct tcgacgccga cgtgatgccg gagacgctgc ggcgcacggc
    13621 gctcggcacg ctcgcggccg gccacccggt gaacctcgag ctggcgctgc gcgcgtccga
    13681 ccgactcggc ggccacatgg tgcagggcca cgtggacgcc acgggcacgg tcgaggcgct
    13741 gcgcgacgag ggcttcgcgc gcgtggtgcg gatcgcggcc ggcacggacg tgctccgcta
    13801 cgtggtcgag aaggggtcga tcgccgtgga cggcgtctcg ctcaccgtgt ccgctgtcgg
    13861 cgacgactgg ttcgaggtat ccctgatccc cgagaccctc gcgcgcacta cgctcggcgg
    13921 cgccgagccc ggtcgcgtgg tcaacctgga ggtggacgtg ctcgctaagt acgtggagaa
    13981 gctggtgagc gcgcgatgag ctcagccgag acgcgcgcgc cgttcagcac catcgaggag
    14041 gcgatcgagg acatccgcgc cgggcggatg gtcgtggtct gcgacgccga ggaccgcgag
    14101 aacgagggcg acctgacgct cgcggcgcag ttcgccacgc ccgacgcgat caacttcatg
    14161 gccaaggagg ggcgcggcct gatctgcctg gcgctcacgc ccgaccgctg cgacgagctc
    14221 gggctcgacc tcatggccgc caagaacgag tcgccctacg agacggcgtt caccgtgtcg
    14281 gtcgaggcgc gcgagggcgt gaccaccggc atctccgcgc acgaccgcgc gcatacgatc
    14341 caggtggcga tcgacccgcg cacgcagccg cgcgacctcg ttcagcccgg gcacgtgttc
    14401 ccgctcaagg cgaaggcggg cggcgtgctg gagcgcacgg gccagaccga ggcggcggtg
    14461 gacctcgcgc ggctcgccgg gctcacgccg gccggggtga tctgcgagat catgaacgac
    14521 gacggcacga tggcccgggt gccggacctc gtcgactact gcgcccggca cgacctgaag
    14581 atggtcacgg tcgccgacct gatcgcctac cggcgccgcc acgacaagct ggtcgagcgc
    14641 gtggtcgaga cgcggctgcc gaccgacttc ggcgacttca gcgcggtggg cttccgttcg
    14701 ctggtggacg gcaagcacca catcgcgatg gtcaagggcg acgtggacgg cgtcgaggac
    14761 gtgctcgtcc gcgtgcactc cgagtgcctg acgggcgacg tgttccactc gcagcgctgc
    14821 gactgcgggc tccagctcga gaccgcgctg gcgcgcatcg actccgaggg ctgcggggtc
    14881 ctgctctacc tcgcgcagga gggtcgcggc atcgggctgc tcaacaagct gaaggcgtac
    14941 aagctccagg aggaggggct cgacacggtc gacgccaacc tcgagctcgg gctgcccgcg
    15001 gacctgcgcg actacggcat cggggcgcag atcctggtgg acctcggact caccacgatc
    15061 cggctgctca ctaacaaccc gaagaagatc gtcgggctcg agggctacgg gctgaaggtg
    15121 agcgaccagc tcccgatcgc gatgccggcg ggcgcgcaca atcgcgacta cctgcgggcc
    15181 aagcgcgaca agctcggcca cctgctccac caccagggcc tcgcgctcga cgaggagatg
    15241 atccacgagg agcaggtcca cgaccgcgag cgcagcgacg gcgccccgcg cgtgccgcgg
    15301 cccgatccgc cggaatgagt gcctgggcga tagtggtcgg gcgcttctac gaggacctgg
    15361 cggacgagct ggtggctggg gcgaagcgcg tcttcgaggg cgaggtcgac gtgtacgacg
    15421 tgccgggggc gttcgagctg ccgctcgggg cgctgtactg cgcgcggtcg ggccgctact
    15481 cgggtgtcgc ctgcctcggc gcggtgattc gcggcgagac ctcgcactac gaccacgtgt
    15541 gcgaggaggc cgccgccggg atcgcgcggg tttcgctcga caccggtgtg ccgtgctcct
    15601 tcggggtact gaccgtcgag aacatggacc aggcgcgcgc acggctcggg gacaaacggc
    15661 atcagggcga ggacgccgcc cgggcggtgg tggcgatggc cgcgatgcgc gaggcgcttg
    15721 cgtgagcgag ctggtcaacc tctggagcga cacgcagacg cgcccgtcgg cgggcatgcg
    15781 ccgcgcgatg gccgaggccg aggtcggcga cgagcagcgc ggcgccgacc cgaccgtgaa
    15841 cgcgctgcag gagcgggtgg ccgagctgct cggccacgag gccgcgctgt tcctcccctc
    15901 gggcacgatg tgcaaccaga tcgggatacg gctgcacgtg cggccgggcg aggcgatgat
    15961 cctgcacggc tcggcgcacc cgatcatcgc cgaggccggg ggccccgccg cgcacgccgg
    16021 cgcgatgatc gtggcggtcg agggcgaggg cgggatgttc gacggcgccg cggtgcgcgg
    16081 ctcgctgagc gtgcccggca accgctacga ggcgcgctcg gcgctcgtga acgtcgagca
    16141 gacgaccaac atgggcggcg gccgggtgtg gccgctggag ctgatgcgcg acgtggtgag
    16201 cgcggcgcgc gcggccgggc tgcgcgcgca cctcgacggg gcgcggctga tgaacgccgt
    16261 ggtggcggcg ggggtgccgg cggccgacta cgcctcgcag ttcgacaccg cctggatcga
    16321 cttcacgaag ggcctcggcg cgcccgtggg cgcgtgcctg gcgggctcgc gcgagctgat
    16381 cgaggaggcg tggcgcctca agcagatgat gggcggcgcg ctgcgccagg cggg
//
LOCUS       DASVPL010000254         7571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_29826, whole genome shotgun sequence.
ACCESSION   DASVPL010000254 DASVPL010000000
VERSION     DASVPL010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29826"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..677)
                     /locus_tag="VF517_13950"
     CDS             complement(162..677)
                     /locus_tag="VF517_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054090.1"
                     /translation="MPAELVGALRALPGLARDIARIAEATRTLPDVRASTAAMERNTS
                     VLPEVVESLEKLGEATSVLIPMDKRMQTIEGAMPVLVEVQQSLAQLPDTMAGLDATLH
                     EMSENLTRLLAALDHLSSDLTTLEDSVGPLGRIAKRLPGGKRSENEDGAAGPPPPDGA
                     ARVDAPPPPTT"
     gene            complement(708..1550)
                     /locus_tag="VF517_13955"
     CDS             complement(708..1550)
                     /locus_tag="VF517_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8054091.1"
                     /translation="MGEVNGDLHTIDELAQRTGLTTRNIRAYQSRGLLPPPEVRGRTG
                     YYGEEHIARLELIREMQADGFNLTAIKRLIEGSGGGWEDMLGFKRIALSGWDTEEPEV
                     ATLEQIAERFGVTPEDDARPLERAIELGVVVPLGGDRFELPSPALIQAGEEVVAAGVP
                     LATALDVLGNIMRHTDAVARTFTRLFLDHVLRPFQERGSPPEEWPQVRQSLERLRPVA
                     GQAISAAFALQMTAAAEETYGRVLGGEGWEPGRGGVPRDGGKRSSRSRPRSHSSRRRR
                     GAGA"
     gene            1645..3555
                     /locus_tag="VF517_13960"
     CDS             1645..3555
                     /locus_tag="VF517_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054092.1"
                     /translation="MRHHRHKLTLALTALAVLAIAPDALAHGDPSKAPSAGGGAVAQI
                     VIGTLAGIVAAGVAAWVIVGHREGRIQLLRRLGAFGERVSGLPSWVAVPVSVHGGGLL
                     IAVFGMYWDIATHIDAGRDEGPFANASHYFILVGLAMIFLAGALACAMPDRKPSDASI
                     RLPRGLQAPLGGVLILICAAVALSAFPLDDMWHRIFGQDVTLWGPTHLLLFGGAALTV
                     VGGFVLVIEGRRAMPPEEVPSKSAAVLRKVSVIGFAGALLIALSTFQGEFDYAVPQFR
                     LVFHPILLMLAAAGALTLVRVHVGRGGALMAVAFFLVVRGLLSLIVSPIFDHTTLHFP
                     LYIVEALMVEAVALRVRPEQTIRFGAIAGAAVGTVGLAAEWAWSHIWWTVEWPSTLLP
                     EAAIAGFVAAVAAGVLGARAGRAVTAEPGLPPVARWATPLAALAVVAVCVYATPIGTG
                     KPEVTADVQLRDVIPGKERAVEVTARLTPADAADDAHWFLVTAWQGEEGRSVVSAMKE
                     ISPGVWRSQEPLPVHGTWKSTLRLHRGSAIEGMAVYFPEDKAIPAKEVPATASFTRPF
                     VEDKKLLQREQKPNASDALVLVGYLIVLLIAIVLVGSIVAGLARLERVTSRPGAKPIE
                     REPLAAAAETAL"
     gene            complement(3647..6205)
                     /gene="valS"
                     /locus_tag="VF517_13965"
     CDS             complement(3647..6205)
                     /gene="valS"
                     /locus_tag="VF517_13965"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX8054093.1"
                     /translation="MSDALPKKPKLEGLEDKWAERWEADGTHRFDRAHAREEIYAVDT
                     PPPTVSGSLHVGHVFSYTHTDVVARFQRMRGREVFYPMGWDDNGLPTERRVQNYFNVR
                     CDPHLQYEPGLEVEPGADGPPRMVSRPNFVELCLKLTTEDEQAFEDLWRHLGLSVDWS
                     MTYTTIGERAQRVAQLSFLGELEQGLIVHVAAPTLWDVGFRTAVAQAELEDRETKGAF
                     HRIRFPNPDGEDVEIETTRPELLPACVALVAHPDDGNYKGLFGSSVRSPLFGAPVPVV
                     AHPLADPEKGTGIAMVCTFGDTTDVTWWRELSLPVRPIVAPDGTLAQVEWGADGFPSD
                     DAARAQSAYDELTGQRIEGARKRIVELLAEDGSLVGEPRPIKHAVKFFEKGDRPIEII
                     TSRQWFVRTTDFQEDFIARGREMEWHPPYMRARFEDWVNGLTGNWCISRQRFFGVPFP
                     VWYPLGEDGVPDYDSPILAARESLPVDPSTDVPPGYEPAQRDVPGGFTADPDVMDTWA
                     TSSLSPQIAGGYPDDPDLFERVFPMDLRPQAHDIIRTWLFSTVVRGQFDHGVAPWTNA
                     AISGWILDPDRKKMSKSKGNVVTPMGMLEQYGSDAVRYWAANGRPGADTAFDEGQMRI
                     GRKLAVKLLNASKFVLGMPPTTPGAITEPVDLSLLARLEAVVEDATRQFERYDYTAAL
                     ERVESFFWFFCDNYLELVKDRRYGSRGDGPAASADAALRVGLSVLQRMFAPFLPFVTE
                     EVWSWWQEGSVHRAAWPTAEEVRSAGSGDAEVLGLAAAVLHEVRKAKSEANLPLRATA
                     LRVEVSAGEPQAGLIELVRDDLREAGVAEEVAVVPGDGGLSVRVELDGTSTAST"
     gene            6261..6647
                     /locus_tag="VF517_13970"
     CDS             6261..6647
                     /locus_tag="VF517_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054094.1"
                     /translation="MKRLLGLMATLIVAAVFAVGCGGDDDDGGGSGTDAPSVGETAPS
                     EGGGSGGTDQPDNKDEAIDRCYEEAKKLEGQARETAEAGCKAADTGDTDQLKDEAKQQ
                     CLDSAKQIPDEAARKQAEQTCDTISK"
     gene            6679..7131
                     /locus_tag="VF517_13975"
     CDS             6679..7131
                     /locus_tag="VF517_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054095.1"
                     /translation="MGLLDRLRGVDSGEGAHAPSLARDAREMRATLTALHSERGGGEL
                     DLRALVRDLLEGEVEAPTADVRHMVALDTSPDEFYAQELAPNWDGLDEDRRADKLDQF
                     VELAHMVEASPDALPRDMAASLRTKLLVLGWAFDEAHGYLGRLAGSSH"
     gene            7189..>7571
                     /locus_tag="VF517_13980"
     CDS             7189..>7571
                     /locus_tag="VF517_13980"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX8054096.1"
                     /translation="MRRTGLALLAAAALCVGVAAPAWSGDEERQPPVAALSVSPQPLV
                     AGDGAVLDGSGSRDPDGSVVSYAWDLDGDGAFERGSGAEARLAHVFEQPGTYRVGLRV
                     VDATGSSADATQNVTVQPRPEPEPKA"
BASE COUNT         1019 a         2753 c         2726 g         1073 t
ORIGIN      
        1 gccgtcgagc gcgggatggc gcggctcgtg gccgcggcgg cggagtccta gcgcgccgtc
       61 ggcggcgccg acaccggccc ggcccggcgc tacgcgccgc gccgacaccg gcccggcccg
      121 gcgctacgcg cgcggcggcg gcgacacctg ccggcccggc gctacgtggt cggcggcggc
      181 ggggcgtcga cccgcgcggc gccgtcgggc ggcggggggc ccgccgcccc gtcctcgttc
      241 tccgagcgct tgccgcccgg cagccgcttc gcgatccgtc ctagcgggcc gaccgagtcc
      301 tcgagtgtgg tgaggtccga gctcaggtgg tcgagcgcgg cgagcagccg cgtcaggttc
      361 tcggacatct cgtggagcgt ggcgtccagt ccggccatgg tgtccggtag ctgcgcgagg
      421 ctctgctgta cctcgacgag gacgggcatc gcgccctcga tggtctgcat ccgcttgtcc
      481 atcgggatga gcacggaggt cgcctcgccg agcttctcca gggactcgac gacctcgggc
      541 agcacgctgg tgttgcgttc catcgccgcc gtggacgcgc gcacatccgg gagcgtcctg
      601 gtcgcctcgg cgatccgcgc gatgtcgcgc gccagccccg gcagggcgcg cagggctccg
      661 accagctccg cgggcatgcc cacgactcgt ccgaacgggt tcatcgccta cgcccccgcg
      721 ccccgccgtc gcctgctgct gtgcgatcgc gggcgcgatc gcgacgagcg cttgccgccg
      781 tcgcgcggca ccccgccgcg gccaggctcc caaccctcgc ccccgagcac ccgcccgtag
      841 gtctcctcgg ccgccgccgt catctggagc gcgaacgcgg ccgagatcgc ctgacccgcg
      901 accggccgca ggcgctcgag cgactggcgc acctggggcc actcctccgg gggcgagccg
      961 cgctcctgga acgggcgcag aacgtggtcg aggaacagcc gcgtgaacgt gcgggccacg
     1021 gcgtcggtgt ggcgcatgat gttcccgagc acgtcgagcg ccgtcgcgag cggcacgccc
     1081 gcggccacga cctcctcgcc cgcctggatg agcgcggggc tcggcagctc gaagcggtcg
     1141 ccgccgagcg gcacgaccac acccagctcg atcgcgcgct cgagcggccg cgcgtcgtcc
     1201 tccggcgtga ccccgaagcg ctcggcgatc tgctcgagcg tcgcgacctc gggctcctcg
     1261 gtgtcccagc ccgacagggc gatccgcttg aagccgagca tgtcctccca gccgccgccg
     1321 gagccctcga tgaggcgctt gatggcggtg aggttgaagc cgtcggcctg catctcgcgg
     1381 atgagctcga ggcgggcgat gtgctcctcg ccgtagtagc cggtcctgcc gcggacctcg
     1441 gggggcggga gcagcccgcg cgactggtac gcgcggatgt tgcgcgtggt cagcccggtg
     1501 cgctgggcga gctcgtcgat ggtgtgcagg tccccgttta cctcgcccac ggggcggata
     1561 ctaagaccag agcgtgacat cgtttggtgt cacatacact cgcgtaaccc aagcaggacg
     1621 ctcgcaccaa agaggggacg actcatgcgc caccatcgtc acaagctcac actggccctg
     1681 accgcgctcg ccgtgctggc gatcgcgccc gacgcgctcg cgcacgggga cccgtcaaag
     1741 gccccgtcgg cgggcggcgg cgcggtggcg cagatcgtga tcggcacgct cgccggcatc
     1801 gtcgccgccg gcgtggcggc gtgggtgatc gtcggccacc gcgaggggcg catccagctc
     1861 ctgcgccggc tcggcgcgtt cggggagcgg gtgagcggcc tgccgagctg ggtcgccgtg
     1921 ccggtcagcg tgcacggcgg cggtctgctg atcgccgtgt tcggcatgta ctgggacatc
     1981 gcgacgcaca tcgacgccgg tcgcgacgag ggcccgttcg cgaacgcctc gcactacttc
     2041 atcctcgtcg ggctcgcgat gatcttcctc gccggcgcgc tcgcgtgcgc gatgccggac
     2101 cgcaagccga gcgacgcgtc gatccgcctg ccgcgcgggc tccaggcccc gctcggcggc
     2161 gtgctgatcc tgatctgcgc cgcggtcgcg ctgtccgcgt tcccgctcga cgacatgtgg
     2221 caccgcatct tcggccagga cgtgacgctc tggggcccca cgcacctgct gctcttcggc
     2281 ggcgcggcgc tcaccgtcgt cggcggcttc gtgctcgtga tcgagggccg ccgcgcgatg
     2341 ccgcccgagg aggtgccctc gaagagcgcg gccgtgctgc gcaaggtctc ggtcatcggc
     2401 ttcgcgggcg cgctgctgat cgcgctgtcc accttccagg gcgagttcga ctacgccgtg
     2461 ccgcagttcc gcctcgtctt ccacccgatc ctgctgatgc tggccgccgc gggcgccctc
     2521 acgctggtgc gggtgcacgt cggccgcggc ggcgcgctga tggccgtggc gttcttcctg
     2581 gtcgtccgcg ggctgctcag cctgatcgtc tcgccgatct tcgaccacac gaccctgcac
     2641 tttccgctct acatcgtcga ggcgctgatg gtggaggccg tcgcgctgcg ggtgaggccc
     2701 gagcagacga tccggttcgg cgcgatcgcc ggcgcggccg tcggcacggt cgggctcgcg
     2761 gccgagtggg cctggtcgca catctggtgg accgtggagt ggccctccac gctgctcccc
     2821 gaggcggcca tcgcgggctt cgtcgccgcg gtcgcagccg gcgtgctcgg agcgcgcgcg
     2881 ggtcgcgccg tgaccgccga accggggctt cctccggtcg cgcgctgggc cacgccgctc
     2941 gccgcgctcg cggtcgtggc cgtgtgcgtc tacgccacgc cgatcggcac gggcaagccc
     3001 gaggtgaccg ccgacgtgca gctgcgcgac gtgatccccg gcaaggagcg cgcggtggag
     3061 gtcaccgccc gcctcacgcc cgccgacgcg gccgacgacg cgcactggtt cctcgtcacc
     3121 gcatggcagg gcgaggaggg ccgctcggtc gtatcggcga tgaaggagat ctcgcccggc
     3181 gtctggcgct cgcaggagcc gctgccggtc cacggcacct ggaagtcgac gctgcggctg
     3241 catcgcggca gcgcgatcga gggcatggcg gtgtacttcc cggaggacaa ggcgatcccg
     3301 gccaaggagg tcccggccac cgcctcgttc acccgcccgt tcgtggagga caagaagctg
     3361 ctccagcgcg agcagaagcc gaacgcatcg gacgccctcg tcctggtcgg ctacctgatc
     3421 gtgctgctga tcgcgatcgt cctggtcggc tcgatcgtcg ccggcctcgc gcggctggag
     3481 cgcgtcacct cgcggccggg cgcgaagccg atcgagcgcg agcccctggc ggcggccgcg
     3541 gagaccgctt tgtagcgagc ctcggcgcat cgattggagc ctttctccgc catgcggtac
     3601 acaaggctcc aatcgatgag aaagcgggcg atccgggccc gcctccctaa gtgctcgccg
     3661 ttgacgtacc gtcgagctcg acccgcacgc tgagcccgcc gtcgcccggc accacggcaa
     3721 cctcctctgc cacccccgcc tcgcgcaaat catcgcgcac cagctcgatc agccccgcct
     3781 ggggctcccc cgcggagacc tccacgcgca aagccgtggc ccgcagcggc aaattggcct
     3841 cggacttggc cttgcgcacc tcgtgcagca cggcggccgc gagccccagc acctcggcgt
     3901 cgccggagcc ggcggagcgg acctcctccg ccgtcggcca tgccgcgcgg tgcaccgacc
     3961 cctcctgcca ccacgaccag acctcctcgg tcacgaacgg caggaacggc gcgaacatcc
     4021 gctggagcac ggacaggccg acgcgaaggg cggcgtcggc ggatgcggcg gggccatccc
     4081 cgcgcgagcc gtagcggcgg tccttcacca gctccaggta gttgtcgcag aagaaccaga
     4141 agaagctctc cacccgctcc agggcggcgg tgtagtcgta gcgctcgaac tggcgcgtgg
     4201 cgtcctcgac cacggcctcg aggcgagcaa gcagcgacag gtcgacgggc tcggtgatcg
     4261 cgccgggcgt ggtcggcggc atcccgagca cgaacttcga cgcgttcagc agcttcacgg
     4321 ccagcttgcg gccgatccgc atctgtccct cgtcgaacgc cgtgtcggcg cccggccgcc
     4381 cgttcgcggc ccagtagcgc accgcgtcgg agccgtactg ctcgagcatc cccatcgggg
     4441 tgaccacgtt gcccttcgac ttcgacatct tcttgcggtc gggatcgagg atccagccgg
     4501 agatcgccgc gttggtccag ggcgcgacgc cgtggtcgaa ctgcccgcgc acgaccgtcg
     4561 agaagagcca ggtcctgatg atgtcgtgcg cctgcgggcg caggtccatc gggaacacgc
     4621 gctcgaacag gtcgggatcg tccgggtagc cgcccgcgat ctgcggcgac agcgacgacg
     4681 tggcccacgt gtccatcacg tcggggtcgg cggtgaagcc gccggggacg tcgcgctgcg
     4741 ccggctcgta gccgggcggc acgtcggtcg acgggtcgac cggcagcgac tcgcgcgcgg
     4801 cgaggatcgg gctgtcgtag tcgggcaccc cgtcctcgcc gagcgggtac cacaccggga
     4861 acggcacgcc gaagaagcgc tggcggctga tgcaccagtt gccggtcagg ccgttgaccc
     4921 agtcctcgaa tcgcgcgcgc atgtacggcg ggtgccactc catctcgcgc ccgcgcgcga
     4981 tgaagtcctc ctggaagtcc gtcgtgcgca cgaaccactg gcggctcgtg atgatctcga
     5041 tcggccggtc gcccttctcg aagaacttga ccgcgtgctt gatcggccgc ggctcgccga
     5101 ccagcgaccc gtcctcggcc agcagctcga cgatccgctt gcgagcgccc tcgatgcgct
     5161 ggccggtgag ctcgtcgtag gcgctctgcg cgcgggcggc gtcgtcggag gggaagccgt
     5221 cggcgcccca ctcgacctgc gcgagcgtgc cgtctggcgc gacaatcggg cgcaccggca
     5281 gcgacagctc gcgccaccac gtcacgtcgg tggtgtcgcc gaacgtgcag accatcgcga
     5341 tgccggtgcc cttctcgggg tcggcgagcg gatgcgcgac gaccggcacc ggcgccccga
     5401 agagggggct gcggaccgag ctcccgaaga gccccttgta gttgccgtcg tcgggatgcg
     5461 cgacgagcgc gacgcaggcc gggaggagct cgggacgggt ggtctcgatc tccacgtcct
     5521 cgccatcggg attcgggaaa cggatccggt ggaacgcgcc cttcgtctcg cggtcctcga
     5581 gctcggcctg ggcgaccgcg gtgcggaagc cgacgtccca cagcgtgggc gccgccacgt
     5641 gcacgatcag gccctgctcg agctcgccga ggaacgagag ctgcgccacc cgctgcgcgc
     5701 gctcgccgat cgtcgtgtag gtcatcgacc agtcgaccga gagcccgagg tggcgccaca
     5761 ggtcctcgaa cgcctgctcg tcctcggtcg tgagcttcag gcacagctcg acgaagttcg
     5821 gccgcgagac cattcgcggc ggaccatcgg cgccgggctc gacctcgagc cccggctcgt
     5881 actggaggtg cgggtcgcag cggacgttga agtagttctg cacgcgccgc tcggtcggca
     5941 gtccgttgtc gtcccagccc atcgggtaga agacctcgcg cccgcgcatc cgctggaagc
     6001 gcgcgaccac gtcggtgtgc gtgtaggaga agacgtgccc gacgtgcagc gagccgctga
     6061 cggtcggcgg cggcgtgtcg acggcgtata tctcctcacg cgcgtgcgcg cggtcgaagc
     6121 ggtgcgtgcc gtcggcctcc cagcgctcgg cccacttgtc ctcgagcccc tcgagcttgg
     6181 gcttcttcgg gagggcgtcg gacattcgct cctacgttac ctgcggatgt ggtctactcc
     6241 gcggcatgaa caaccaaggc atgaagcgac tactcggact gatggccaca ctgatcgtcg
     6301 cggcggtgtt cgccgtgggc tgtggcgggg acgacgacga cggcggcggt agtgggaccg
     6361 acgcgccaag cgtgggtgag accgctccct ccgagggcgg cggctccggc ggcaccgatc
     6421 agccggacaa caaggacgag gcgatcgaca ggtgctacga ggaggccaag aagctggagg
     6481 gccaggctcg tgagacggcg gaggccggct gcaaggccgc cgacaccggc gatacggacc
     6541 agctcaagga cgaggcgaag cagcagtgcc tcgactccgc gaagcagatc ccggacgagg
     6601 cagcacgcaa gcaggccgag caaacctgcg acacgatcag caagtagccg atcgcgtcaa
     6661 cggttcggaa gagaggccat gggtctgctg gaccggctga ggggcgtcga ctccggtgag
     6721 ggtgctcacg cgccctcgct agcgcgcgac gcccgtgaga tgcgagccac cctcaccgcg
     6781 ctccacagcg agcgcggtgg gggggagctc gacctgcgcg cgctcgtgcg cgacctgctc
     6841 gagggcgagg tcgaggcccc gaccgccgac gtgcgccaca tggtcgcgct cgacacctcg
     6901 ccggacgagt tctacgcgca ggagctcgca ccgaactggg acgggctcga cgaggaccgc
     6961 cgcgcggaca agctcgacca gttcgtggag ctcgcgcaca tggtcgaggc gtcgcccgac
     7021 gcgctcccgc gcgacatggc ggcctcgctg cggaccaagc tgctcgtgct cggctgggcg
     7081 ttcgacgagg cgcacggcta cctcggccgc ctggccgggt cgagccactg acccgaaccg
     7141 gggcgcctcc acatccgtac gcccccatat gcgactaagc tcgccacggt gaggcgtacc
     7201 ggactcgcac tcctggccgc cgcggcgctc tgcgtgggcg tcgccgcgcc cgcctggtcc
     7261 ggcgacgagg agcgccagcc gccggtggcg gcgctgagcg tctcgccgca gccgctcgtc
     7321 gcgggcgacg gggccgtgct cgacgggtcc ggctcgcgcg accccgacgg gtcggtcgtg
     7381 agctacgcgt gggacctcga cggcgacggt gcgttcgagc gcggcagcgg agccgaggcg
     7441 cggctcgcgc acgtgttcga gcagcccggc acctaccgcg tcgggctgcg tgtggtcgac
     7501 gcgaccgggt ccagcgccga cgcgactcag aacgtgacgg tgcagccgcg gccggagccc
     7561 gagccgaagg c
//
LOCUS       DASVPL010000255        10741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_226040, whole genome shotgun sequence.
ACCESSION   DASVPL010000255 DASVPL010000000
VERSION     DASVPL010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226040"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..697
                     /gene="ruvC"
                     /locus_tag="VF517_13985"
     CDS             197..697
                     /gene="ruvC"
                     /locus_tag="VF517_13985"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX8054097.1"
                     /translation="MVVVLGIDPGAASTGYGVVVSSGGRMAALDGGVIETSADEPLER
                     RLTRIHAQVCDLIAEHAPAAMAVEDIFFGQNVRTAFSVGQARGVVLLSAGMAGVPCYS
                     YTPQAVKSAVCGSGRAEKGQVQRMVGALLSLPEPPEPDHAADALAVAICHANGVAMRT
                     AVGSAS"
     gene            694..1269
                     /gene="ruvA"
                     /locus_tag="VF517_13990"
     CDS             694..1269
                     /gene="ruvA"
                     /locus_tag="VF517_13990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX8054098.1"
                     /translation="MIASVRGEVIVRRPDHVVVECGGVGYRLAVSANTLKAVPRVEEE
                     IRLHTHLVMRDDGMHLFGFASEPERELFLMLIGVQSVGPKVALAVLSGGTPRELLSAI
                     AGGDSARFQAVPGIGKRTAERIIVELREKVAGATSGDEIVVTRADDPRSLAREGLLGL
                     GFTVQEADQLLDSARGETAEDLIAEALRGAR"
     gene            1266..2330
                     /gene="ruvB"
                     /locus_tag="VF517_13995"
     CDS             1266..2330
                     /gene="ruvB"
                     /locus_tag="VF517_13995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX8054099.1"
                     /translation="MSGIKTPGAEALRDEAEPARIGAAAAAPPDEELDRSLRPKRLED
                     FVGQPAVKEQLAVFVAAARARGEPLDHVLLAGPPGLGKTSLAQIVASELDVPFVQTAG
                     PALERKGDIASFLTALAPGAVFFVDEIHRLPRALEETFYPAMEDRRLPITVGQGAGAR
                     VVTLDLPPFTMIGATTRAGLLTTPLRDRFGVTHRLELYPPEELARIVLRSASILGVEI
                     DTPGAEAIAARSRGTPRVANRLLKRVRDYAEVRGAGRIDAAVARSALELLEVDEAGLD
                     RLDREILRTICEKFEGGPVGLSTLAVAVAEEADTLEDVYEPYLLQKGLIKRTPRGRVA
                     TASAWEHLGLERPRGAASLF"
     gene            2390..2836
                     /locus_tag="VF517_14000"
     CDS             2390..2836
                     /locus_tag="VF517_14000"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8054100.1"
                     /translation="MPNFICPNCGKRSTAAERTAGFRSHARGCANCGFGFLFELLDDY
                     YPAPNAAFFVCDAQAQVIAAGRGARELTGLGDVDVMGRPVREVLGLEFENGSDHIGTA
                     LEWGVRVLDKPVVVHAEGDRPEPAKADIFPAYDEDGGLLLVLTPKL"
     gene            2833..6132
                     /gene="secD"
                     /locus_tag="VF517_14005"
     CDS             2833..6132
                     /gene="secD"
                     /locus_tag="VF517_14005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX8054101.1"
                     /translation="MTDRRRNLIILGIVGVLLTLAALVILPPSPVSKETRLGLDLKGG
                     VELVYQARPTPKAPKITPEAINDAISTIRKRTDSLGVSEPEIQRAGENEISIGLPAVK
                     NAARAEEQVGTTAQLQFYDWEPNVLGNPYQPINDFVQAVELGAASKPRAEDIDIPAGG
                     ASDAIEKRFGGDKAKIRAYYDGQNDSVGDKYYVFGANKQLIRPGGVGPTEGDLSDRND
                     ATAFYGSCSEIAEDFSPTAARPREPEKAAADEKAKPAKDTACPQTMDKLVGQPGAPGN
                     GSRVVKVPRGIVVVKAEEPQGATARTPPQYFVIEDDSELSGSEIENPEQNFDQQTQEP
                     IVTMEFTDTGKKAFARVTKRIAERGQSTLPQPGSKPEDRFQRFMISLDGQIVSLATID
                     FLRNPEGIDGETGAQINGIGNIEDTQDLANNLRIGALPVSLKLISKTQVSATLGQQAL
                     DQGLFAAGIGLILTLLFLIAFYRVLGVIAGSALVIYAVLLFALVKLIPITLTLPGIAG
                     LVLTLGVAADANIVIFERVKEEARAGRTIPAAIANGYTKALRTIIDANVVTIGVAFIL
                     FTLATSTVKGFAFTLGIGTLVSLFTAVLATSAILGTFSRSRVLGSRYAIGIGKRESEW
                     RFDFTGMSKWFFSMSGMILLAGALAVAGLGVNFGIDFESGTRITTPLERKASVSDVRD
                     TLDPLGYSDAKIQEVEDPELGENIVQIATQQLEPERVTVVQQALDRDFGVTNFSSTSV
                     GPTFGQQIARTAAIAIVASLILISIYIGLRFEFKFAVPVLIALAHDLLITAGVYALTE
                     REVNTATVAALLTILGYSLYDTIIVFDRIRENVPRMPRATFSQIVNRSMSEVLTRSIV
                     TSSSTLMPIVALMVFGGATLQDFGFALLIGVASGTYSSVFIAAPVLVHWKERERVYRR
                     RRRMIMDEHGGEVPPFADIGAPGEDVEPEHKAGRVERAAATRRQRRAAKAAEQRARQT
                     SRPEPAAADEPAAGGRPVPERGAEEVVAPVATEDPAEIPPSPAAEAAAASEASEAEPV
                     ADPSGVRARAAARAAARRGTSTRPAPKRDGDGDGSGGADGADGSDGGNGGGLGSSSKP
                     DNKRRKRHGRR"
     gene            6129..6413
                     /locus_tag="VF517_14010"
     CDS             6129..6413
                     /locus_tag="VF517_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054102.1"
                     /translation="MSMLVWVMMALAVWHFTVFLPDYFWGGIVGALLCAIFGAALIGF
                     LISGLSVPGRHDTDLAQALVALPGTFAGLAFAYLYGRAQDRKADIERLPL"
     gene            6533..9142
                     /gene="recJ"
                     /locus_tag="VF517_14015"
     CDS             6533..9142
                     /gene="recJ"
                     /locus_tag="VF517_14015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX8054103.1"
                     /translation="MPSQAARWTCKPYSVAAAERIAHDLDVSPPVATVLVRRGYATPE
                     AAREFIEAGDRHNPRAFAGIDVACSLILDHVRRGSPIVVHGDYDVDGVCSTAIVLGVL
                     RRLGADPRWHIPAREDGYGLSTATVERLAASGAGLIVTTDCAVGAVEEVARARELGVD
                     VVVTDHHRPGEVLPDCPIVHPVICGYPFPDLCAAGVAHKLAEALLTRAGADPAGADAD
                     LDLVALATVADVVSLTGENRRLVRDGLRAMARTERPGLRALMRVASVDLGGVTEHALG
                     FRLGPRLNAAGRLARAEAALELLMTRDEGRAEEVARELDELNRRRQDTETRILFGAEA
                     ARAEHPDAPAYVLAGEGWHPGVIGIVASRMVERYHRPCVMIALDGNDGKGSGRSISAF
                     DLHAGLGACAPHLRRFGGHRAAAGLEIDAGAVDAFRDAFIAHASTKLSPSDLVRVERI
                     DAVVGADAVGTALAEELERLGPFGHGNPQPALLVPATRVSEVRGMGDEDQHSRLTIAS
                     GGGARARAVAFRTSPGALNAEPDARYDAAVRLELNEWNGTVEPRLVVRSLCPTERGDC
                     RPLLAEDDFWTEFARELEAEPGGFANHPAAPAAPAPGASAPHTAAAPPPAPGGFAEHP
                     AGAVPAGAAPRTVRDRRGEGFAGVAGDVLSSGESVAILCADVARRRPGLEAIVAGIAR
                     AVSGGHEDESRCPALISWDALAADPACAREHAHLIALDPPRTLAGEHLLATAPAPAGG
                     GFVHLAWGAAEAEFALASARHDLDLRPHATAIYRDLRATGPCAGRQLESLLRGPGPHP
                     RAAALCARAMRVLVELGLAACEPTPAGGFAARVSEAARTELDRSPTYRASAQRLAEAE
                     RELAAAAAISRAA"
     gene            complement(9370..10440)
                     /gene="wecB"
                     /locus_tag="VF517_14020"
     CDS             complement(9370..10440)
                     /gene="wecB"
                     /locus_tag="VF517_14020"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX8054104.1"
                     /translation="MRVATVVGNRPQFVKAAAVSRLLREHHDEVLVHTGQHYDDEMSR
                     VFFDELPIPAPDRQLGVGSGAAAVQTARMLEALEGVLREIEPALVLVYGDTNSTLAGA
                     LAAAQARVPVAHVEAGMRSFDRAMPEELNRVLTDHASDLLLCSTETAIANLAREGIEA
                     GVHLVGDAMADVALATARVARSRSDVLEGFGVEPGGYLLVTAHRAGNVDAPERLAMLV
                     ELLLSLPRPLVFPVHPRTRARLEAAGLLAALEEAPGVHLSAPVGYVELLALAIGARAV
                     VTDSGGLQKEAYLVEVPCVTLRDTTEWVETVETGWNVLVDLDAAAAQAALDRPPPADH
                     PDLYGGGRAGERIRDVIDAFGA"
     gene            10468..>10741
                     /locus_tag="VF517_14025"
     CDS             10468..>10741
                     /locus_tag="VF517_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8054105.1"
                     /translation="MAVPLFASSLEPHHDALVERMAAVVRSGRYILGPEVRAFEDEYA
                     TYLGARHCVGVANGTDALTIALRALGVGPGDEVVCPSFTFYATAEAI"
BASE COUNT         1441 a         3950 c         3911 g         1439 t
ORIGIN      
        1 gggtgaggcc cgggcgtgcc ggcgtccagc gcgctcgcgc cacgcgctcc gacctgcggc
       61 cgggcaaggc gaacatccgg gtgcttcgac gctagtcgtc ctcgattgga gccttttgcc
      121 gcgtatagcc ggctaaggct ccagtcgctg cgcccgccag cgggaggcgt ccggctccga
      181 gcgcagaatc acccccgtgg tggtcgtcct cggcatagat cccggtgctg caagcaccgg
      241 ttacggcgtc gtcgtctcga gcgggggccg catggccgcg ctcgacggtg gcgtcatcga
      301 gacgtcggcc gacgagccgc tcgagcgccg gctcacgcgc atccacgcgc aggtctgcga
      361 cctgatcgcc gagcacgcgc ccgcggcaat ggcggtcgag gacatcttct tcggccagaa
      421 cgtgcgcacc gcgttctcgg tcggtcaggc gcgcggggtc gtcctgctgt cggccggtat
      481 ggctggggtc ccgtgctact cgtacacgcc gcaggcggtc aagtccgcgg tgtgcgggtc
      541 cggtcgggcg gagaaggggc aggtgcagcg gatggtcggc gccctgctgt cgctgccgga
      601 gccgcccgag cccgatcacg cggccgacgc gctggcggtc gccatctgcc acgccaacgg
      661 cgtcgccatg cgcaccgcgg tggggagcgc gtcgtgatcg cgtcggtgcg cggcgaggtg
      721 atcgtgcggc gcccggacca cgtggtggtg gaatgcggcg gggtcggcta ccggctggcg
      781 gtgtcggcca acacgctgaa ggcggtgccg cgggtggagg aggagatccg gctccacacg
      841 cacctcgtga tgcgcgacga cggcatgcac ctcttcgggt tcgcctcgga gcccgagcgc
      901 gagctcttcc tgatgctgat cggcgtgcag agcgtgggcc cgaaggtcgc gctcgccgtg
      961 ctctccggcg gcacgccacg cgagctgctg agcgcgatcg cgggcggcga ctcggctcgc
     1021 ttccaggccg tccccggcat cggcaagcgc accgcggaga ggatcatcgt cgagctgcgc
     1081 gagaaggtcg cgggggcgac gagcggcgac gagatcgtcg tcacgcgcgc cgacgacccg
     1141 cgctcgctcg cgcgcgaggg cctgctcggc ctcgggttca cggtccagga ggccgaccag
     1201 ctgctcgaca gcgcccgcgg cgagacggcg gaggacctga tcgccgaggc cctgcgaggc
     1261 gcgcgttgag cggcatcaag acccccgggg ccgaggcgct gcgcgacgag gccgagcctg
     1321 cccgcatcgg cgcggccgcg gcggcgccgc cggacgagga gctcgaccgg tcgctgcgcc
     1381 cgaagcggct cgaggacttc gtcggtcagc cggccgtcaa ggagcaacta gccgtgttcg
     1441 tcgcggccgc tcgcgcgcgc ggggagccgc tcgaccacgt gctgctcgcg ggcccgccgg
     1501 gtctcggcaa gacctcgctg gcgcagatcg tcgcgtcgga gctcgacgtc ccgttcgtgc
     1561 agaccgccgg ccccgccctc gagcgcaagg gcgacatcgc ctcgttcctc accgccctgg
     1621 cgcccggcgc ggtcttcttc gtcgacgaga tccaccgcct cccgcgcgcg ctggaggaga
     1681 cgttctaccc cgcgatggag gatcgccgcc tgccgatcac cgtcggccag ggcgccggcg
     1741 cgcgcgtggt cacgctcgac ctcccgccat tcacgatgat cggcgccacc acccgggcgg
     1801 gactgctcac cacgccgctg cgcgaccgct tcggcgtgac ccaccggctc gagctctacc
     1861 cacccgagga gctcgcgcgg atcgtgctgc gctcggcgag catcctgggg gtcgaaatcg
     1921 acacacccgg cgccgaggcg atcgcggccc gctcgcgcgg cacgccgcgc gtagccaacc
     1981 gcctgctcaa gcgcgtacgc gactacgccg aggtgcgcgg cgccggtcgc atcgatgccg
     2041 ccgtggcgcg ctccgcgctc gagctcctgg aggtcgacga ggccgggctc gaccggctcg
     2101 accgcgagat cctcaggacc atctgcgaga agttcgaggg cggcccggtc gggctctcga
     2161 cgctcgcggt cgcggtcgcc gaggaggccg acacgctcga ggacgtctac gagccctacc
     2221 tactccagaa ggggctgatc aagcgcacgc cgcgcggtcg cgtggccacg gcgtccgcct
     2281 gggagcacct ggggctggag cgcccacgcg gcgcggcgag cctgttctga gtgtgggtcg
     2341 ccgccgcaca gaaaacgttt aggcatgacg gcctaacctc taggcgtcca tgccgaactt
     2401 catctgcccg aactgcggca agcgcagcac cgcggccgag cggacggccg gctttcgcag
     2461 ccacgcccgc ggctgcgcca actgcggctt cggcttcctc ttcgagctgc tcgacgacta
     2521 ctaccccgcg cccaacgccg cgttcttcgt gtgtgatgcg caggcccagg tgatcgcggc
     2581 gggccgcggc gcgcgcgagc tcacggggct cggcgacgtc gacgtgatgg gtcgcccggt
     2641 ccgcgaggtg cttgggctcg agttcgagaa cggctcggac cacatcggca cggcgctcga
     2701 gtggggcgtg cgcgtgctcg acaagcccgt cgtggtgcac gcggagggcg accggcccga
     2761 gcccgccaag gctgacatct tcccggctta cgacgaggac ggcgggctcc tcctcgtgct
     2821 cacgccgaaa ctgtgaccga ccgccgccgc aacctgatca tcctcggcat cgtgggcgtg
     2881 ctgctcacgc tggcggccct ggtgatcctc ccgccgtcgc cggtgtcgaa ggagacgcgg
     2941 ctgggcctcg acctgaaggg cggagtcgag ctcgtctacc aggcccggcc cacgcccaag
     3001 gcgccgaaga tcacgccgga ggcgatcaac gacgcgatct ccacgatccg caagcgcacc
     3061 gactcgctgg gcgtctccga gcccgagatc cagcgcgccg gcgagaacga gatctcgatc
     3121 gggctgccgg cggtcaagaa cgccgcccgc gcggaggagc aggtcggcac cacggcgcag
     3181 ctccagttct acgactggga gccgaacgtc ctgggcaacc cgtaccagcc gatcaacgac
     3241 ttcgtccagg cggtggagct cggcgcggcg tccaagccgc gcgccgagga catcgacatc
     3301 ccggccggag gcgcgtcgga cgcgatcgag aagcgcttcg gcggcgacaa ggcgaagatc
     3361 cgcgcctact acgacggcca gaacgactcg gtcggcgaca agtactacgt ctttggggcg
     3421 aacaagcagc tgatccggcc cggcggcgtc gggcccacgg agggcgacct ctcggaccgc
     3481 aacgacgcca cggcgttcta cgggtcctgc agcgagatcg ccgaggactt ctcgcccacg
     3541 gccgcgcgcc cgcgcgagcc cgagaaggcg gccgccgacg agaaggcgaa gcccgcgaag
     3601 gacaccgcct gcccgcagac gatggacaag ctggtcggcc agcccggcgc ccccgggaac
     3661 ggcagccgtg tcgtgaaggt gccgcgcggc atcgtcgtcg tcaaggccga ggagccacag
     3721 ggggccaccg cgcgcacgcc gccgcagtac ttcgtgatcg aggacgactc cgagctgtcg
     3781 ggctccgaga tcgagaaccc ggagcagaac ttcgaccagc agacccagga gcccatcgtc
     3841 acgatggagt tcacggacac cgggaagaag gcgttcgccc gggtcacgaa gcggatcgcc
     3901 gagcgcgggc agtccacgct gccgcagccg ggcagcaagc ccgaggaccg cttccagcgc
     3961 ttcatgatct cgctggacgg ccagatcgtc tcgctggcga cgatcgactt cctgcgcaac
     4021 cccgagggga tcgatggcga gaccggcgcc cagatcaacg gcatcggcaa catcgaggac
     4081 acgcaggacc tcgcgaacaa cctgcggatc ggcgcgctgc cggtctccct caagctgatc
     4141 tcgaagacgc aggtgtcggc cacgctcggc cagcaggcgc tcgaccaggg cctgttcgcg
     4201 gcggggatcg gcctgatcct cacgctcctg ttcctgatcg cgttctaccg cgtgctcggc
     4261 gtgatcgccg gctccgcgct cgtgatctat gcggtcttgc tgttcgcgct cgtgaagctg
     4321 atcccgatca cgctcacgct cccgggcatc gccggcctcg tcctcacatt gggggtggcc
     4381 gccgacgcga acatcgtcat cttcgagcgc gtcaaggagg aggcgcgcgc ggggcggacg
     4441 atcccggccg ctatcgcgaa cggctacacg aaggcgctgc gcacgatcat cgacgcgaac
     4501 gtcgtgacga tcggcgtcgc gttcatcctc ttcacgctcg cgacctcgac cgtcaagggc
     4561 ttcgcgttca cgctcggcat cggcacgctc gtgtcgctgt tcacggcggt gctcgccacc
     4621 tcggcgatcc tcggcacgtt ctcgcgctcg cgggtgctcg gctcgcgcta cgcgatcggg
     4681 atcggcaagc gcgagagcga gtggcgcttc gacttcaccg gcatgtcgaa gtggttcttc
     4741 tcgatgtcgg gcatgatcct gctggccggt gcgctggcgg tggcgggact cggcgtgaac
     4801 ttcggcatcg acttcgagtc gggcacgcgc atcaccacgc cgctcgagcg caaggcgagc
     4861 gtcagcgacg ttcgcgacac cctcgacccg ctcggctact ccgacgcgaa gatccaggag
     4921 gtcgaggacc ccgagctggg cgagaacatc gtgcagatag cgacccagca gctcgagccc
     4981 gagcgggtca ccgtggtgca gcaggcgctc gaccgcgact tcggcgtcac gaacttctcg
     5041 agcacgtcgg tcggcccgac gttcgggcag cagatcgcgc gcaccgcggc catcgcgatc
     5101 gtcgcgtcgc tgatcctgat ctcgatctac atcgggctca ggttcgagtt caagttcgca
     5161 gtgccggtgc tgatcgcgct ggcgcacgac ctgctgatca cggcgggtgt gtacgcactg
     5221 acggagcggg aggtgaatac ggcgaccgtg gccgcgctgc tcactatctt gggctactcg
     5281 ctctacgaca cgatcatcgt gttcgaccga atacgcgaga acgtgccgcg catgccgcgg
     5341 gcgaccttct ctcagatcgt gaaccgctct atgagcgagg tgctcacgcg ctcgatcgtc
     5401 acgagctcgt cgacgctgat gccgatcgtc gcgctgatgg tcttcggcgg cgcgacgctc
     5461 caggacttcg gcttcgcgct gctgatcggc gtggcgtccg gtacgtactc gtcggtgttc
     5521 atcgccgcgc cggtgctcgt gcactggaag gagcgcgagc gggtctacag gcgccggcgc
     5581 cggatgatca tggacgagca cggcggcgag gtgcccccgt tcgcggacat cggggcaccg
     5641 ggcgaggacg tcgagcccga gcacaaggcg ggccgggtcg agcgcgcggc cgccacgcgg
     5701 cgccagcgcc gggcggccaa ggccgccgag cagcgagcgc ggcagacctc gcggcccgag
     5761 ccggcggcgg cggatgagcc cgcggcgggc ggccggccgg tgcccgagcg cggggcggag
     5821 gaggtcgtgg ccccggtcgc gaccgaggac ccggcggaga tcccgccctc gccggcggcg
     5881 gaggccgcgg cagcttcgga ggcctcggag gcggagccgg tggccgaccc gagcggcgtg
     5941 cgagcacgcg cagcggcgcg tgcggcggcc cgccgaggca cgagcacgcg gcccgcgccc
     6001 aagcgcgacg gtgacggcga cgggtcgggc ggcgctgatg gtgccgacgg ctcggatggc
     6061 ggcaacggcg gcgggctcgg gtcgtcctcg aagcccgaca acaagcgacg caagaggcac
     6121 ggccgccgat gagcatgctc gtctgggtca tgatggccct ggccgtctgg cacttcacgg
     6181 tcttcctgcc cgactacttc tggggcggca tcgtgggggc gctgctctgc gcgatcttcg
     6241 gcgcggcgct catcggcttc ctgatctcgg gcctgtccgt gcccggccgc cacgacaccg
     6301 atctggccca ggcgctcgtc gcgctgcccg gcaccttcgc cggcctcgcg ttcgcgtacc
     6361 tgtacgggcg cgcgcaggac cgcaaggcgg acatcgagcg cctgccgctg tagcggcgac
     6421 gccgctcggc ggcgcgcgag ctcccgcggc gccgcgctga ccgcccttcg gctccgcccc
     6481 gccgtgaccg gcgttcgtgc cttccgtccg gctccggacc gaccatggcc ggatgccttc
     6541 gcaagccgcg cgctggacct gcaagcccta ctcggtggca gccgccgagc ggatcgcgca
     6601 cgatctggac gtatccccgc ccgtcgccac ggtgctcgtc cggcgcggat acgccacgcc
     6661 cgaggccgcg cgggagttca tcgaggcggg tgaccggcac aatccgcgcg cgttcgcggg
     6721 gatcgatgtc gcatgcagcc tcatcctcga ccacgtgcgg cgcgggtcgc cgatcgtggt
     6781 ccacggcgac tacgacgtcg acggcgtctg ttcgaccgcg atcgtgctcg gcgtgctgcg
     6841 ccgcctcggc gccgacccgc gctggcacat ccccgcgcgc gaggacggct acgggctgtc
     6901 gacggcgacg gtcgagcggc tcgccgcgag cggcgcgggc ctgatcgtca cgacggactg
     6961 tgcggtcggc gcggtggagg aggtggcgcg tgcgcgcgag ctgggggtgg acgtggtcgt
     7021 caccgaccac caccggcccg gcgaggtgct gcccgactgc ccgatcgtcc acccggtgat
     7081 ctgcggctac ccgttcccgg acctgtgcgc cgccggggtc gcgcacaagc tcgcggaggc
     7141 gctcctgacc agggcggggg ccgacccggc gggcgcggat gccgacctcg acctggtcgc
     7201 cctcgcgacg gtcgccgacg tggtgtcgct caccggcgag aaccgccggc tcgtccgcga
     7261 cggcctgcgc gcgatggctc gcaccgagcg ccccgggctg cgcgcgctga tgcgcgtcgc
     7321 gtcggtcgat ctcggcggcg tgaccgagca cgcgctcggg ttccggctcg gaccgcggct
     7381 gaacgcggcg gggcggctcg cgcgcgcgga ggccgccctc gagctgctga tgacgcgtga
     7441 cgagggacgc gccgaggagg tcgcgcgcga gctcgacgag ctcaaccggc gccgtcagga
     7501 caccgagacc cgcatcctct tcggcgccga ggccgcgcgg gccgagcacc cggatgcgcc
     7561 ggcgtacgtg ctcgcggggg aggggtggca tcccggcgtg atcgggatcg tggcctcgcg
     7621 gatggtcgag cgctaccacc gcccgtgcgt gatgatcgcc ctcgacggca acgacgggaa
     7681 ggggtccggg cgcagcatct ccgccttcga cctgcacgcc gggctcggcg cctgcgcccc
     7741 gcacctgcgc cggttcggag gccaccgtgc cgcggccggg ctcgagatcg acgcgggggc
     7801 cgtcgacgcc ttccgcgacg ccttcatcgc ccacgcctcc acgaagctct cgccgagcga
     7861 cctcgtgcgc gtggagcgga tcgacgcggt ggtgggcgcc gacgccgtcg gtaccgccct
     7921 cgccgaggag ctcgagcggc tcggaccgtt cgggcacggc aacccgcagc cggcgctgct
     7981 cgtgccggcc acgcgcgtga gcgaggtgcg cgggatgggg gacgaggacc agcactcgcg
     8041 gctcacgatc gcctcgggcg ggggcgcgcg ggcccgtgcg gtggcgttcc gcacgtcgcc
     8101 gggggcgctg aacgcggagc cggacgcgcg ctacgacgcc gccgtgcgcc tcgagctcaa
     8161 cgagtggaac ggcaccgtgg agccgcgcct ggtggtgcgg agcctgtgcc cgaccgaacg
     8221 cggcgactgc cggccgctac tggccgagga cgacttctgg accgagttcg cgcgcgagct
     8281 cgaggcggag cccggcggct tcgccaacca tcccgccgct ccggcagcgc cggcccccgg
     8341 ggcctccgcc ccacacaccg cggctgcgcc cccgccggcg cccggcggct tcgccgagca
     8401 ccccgccggt gccgtgcccg ccggcgcggc tccgcggacc gtgcgcgacc ggcgcggtga
     8461 ggggttcgcg ggcgtcgccg gtgacgtgct ttcgagcggc gagtccgtgg cgatcctctg
     8521 cgcggacgtc gcgcggcggc gcccgggact ggaggcgatc gtggccggca tcgcgcgtgc
     8581 cgtgagcggc ggccacgagg atgagagccg ctgcccggcg ctcatctcct gggacgcgct
     8641 cgccgccgac cccgcttgcg cacgcgagca cgcccacctg atcgcactcg acccgccgcg
     8701 cacgctggcc ggcgagcacc tgctggctac ggcgcccgcg ccggccggcg gcggcttcgt
     8761 ccacctcgcc tggggcgccg cggaggccga gttcgcgctg gccagcgcgc gacacgacct
     8821 ggacctgcgc ccgcacgcga ccgcgatcta ccgcgacctg cgcgcgaccg ggccgtgcgc
     8881 cggccgccag ctcgaatcgc tgctgcgagg ccccggcccg catccgcgcg cggctgctct
     8941 gtgtgcccgc gcgatgcgcg tgctggtgga gctgggactg gcggcctgtg agccgacacc
     9001 ggcgggcggc ttcgctgcgc gcgtgagcga ggcggcccgc acggagctcg accgctcgcc
     9061 gacctaccgc gccagcgcgc agcgcctggc cgaagccgag cgcgagctcg cggccgcggc
     9121 ggcgataagc cgcgcggcct gaccgagcga gccgcccgca gcggcctgac cgagcgggcc
     9181 gctttggcga ccggtcgctt tcgtccgccg gccgcgtcgc cacaccttcg gcagcaaccg
     9241 aagggttgag tcgacattcc tgactttgcc acccatacgg aggcaaagtc aggactgtcg
     9301 ggtctgcggc cgccgtggcc gcgccggcgg tcggggccga atgtggcccg ccgccgcccc
     9361 cggcggccgt caggcgccga aggcgtcgat cacgtcgcgg attcgctcgc cggcgcggcc
     9421 gccgccgtat aggtcggggt ggtccgccgg cggcgggcgg tcgagcgccg cctgcgccgc
     9481 cgcggcgtcg aggtccacga gtacgttcca gccggtctcg accgtctcga cccactccgt
     9541 cgtgtcgcgc agcgtcacgc acggcacctc gacgaggtac gcctccttct ggagcccacc
     9601 ggagtcggtg accaccgcgc gggcgccgat ggcgagcgcc agcagctcca cgtagccgac
     9661 gggggccgag agatgcacgc ccggcgcctc ctcgagcgcg gcgagcagcc cggcggcctc
     9721 cagccgcgcc cgcgtgcgcg ggtgcaccgg gaacaccagt ggccgcggca gcgagagcag
     9781 cagctccacg agcatcgcga gccgctccgg cgcgtccacg ttcccggcgc ggtgggcggt
     9841 tacgagcaga tagccgcccg gctcgacgcc gaacccctcg agcacgtcgc tgcgcgaccg
     9901 cgcgacccgc gccgtcgcca gcgcgacgtc agccatcgcg tcgcccacca ggtgcacgcc
     9961 cgcctcgatc ccctcgcgag cgaggttcgc gatcgccgtc tcggtcgagc acaggagcag
    10021 gtcgctcgcg tggtcggtca gcacccggtt cagctcctcc ggcatcgctc ggtcgaacga
    10081 gcgcatcccg gcctccacat gcgccaccgg cacccgcgcc tgtgccgcgg ccagcgcgcc
    10141 cgcgagcgtc gagttcgtgt cgccgtagac gaggacgagc gccggctcga tctcgcgcag
    10201 aaccccctcg agcgcctcga gcatccgcgc ggtctgcacg gctgcggccc ccgagccaac
    10261 cccgagctgg cgatcgggcg ccgggatcgg cagctcgtcg aagaacaccc gcgacatctc
    10321 gtcgtcgtag tgctggccgg tgtgcaccag cacctcgtcg tggtgctcgc gcaacagccg
    10381 cgaaaccgct gccgccttca cgaactgagg gcggtttccc acgacggtcg cgacgcgcat
    10441 caggcggccg gtacagtagc ggcgccagtg gccgtccccc tcttcgcgag ctcgctcgag
    10501 ccccaccacg acgcgctggt cgagcggatg gcggccgtgg ttcgctcggg ccgctacatc
    10561 ctcggcccgg aggttcgcgc cttcgaggac gagtacgcga cgtacctcgg cgcccgccac
    10621 tgcgtggggg tggcgaacgg caccgacgcg ctgacgatcg ccctgcgggc gctcggggtc
    10681 ggccccggcg acgaggtcgt gtgcccgtcg ttcaccttct acgcgaccgc cgaggccatc
    10741 g
//
LOCUS       DASVPL010000256         7069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_30075, whole genome shotgun sequence.
ACCESSION   DASVPL010000256 DASVPL010000000
VERSION     DASVPL010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30075"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VF517_14030"
     CDS             <1..577
                     /locus_tag="VF517_14030"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8054106.1"
                     /translation="ADAAGLTNVTTRELDLESIDEAADAYDAVLCREGLMLTSDPARA
                     SAEIARVLRPSGRFALSVWGPRERNPWLGLLLDSAGAVLGVPVPPPGVPGPFSLSDRD
                     RLAAVLAGAGLAEVQVDEASVPRSAGSFEEWWQRTVALAGPLAKMLESLPPETRDAIR
                     QRAEEAVRPFATADGGLEFPGVALVASGRAA"
     gene            complement(581..1360)
                     /locus_tag="VF517_14035"
     CDS             complement(581..1360)
                     /locus_tag="VF517_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8054107.1"
                     /translation="MCVAGLFAAGCGGGGEVRTGDPEAHGTSVVRYEIDSRYAGRTLS
                     QVGVTPPDSRRRPPLLVFLHGRGKGGAESNVNGAFLDALRALGPRAPAVVFPDGGDHS
                     YWHDRRGGAWGRYVVREVIPQAVRRLRADPRRVAIGGISMGGFGAFDIALHSPSRFCA
                     VGGHSPAIWASAGETAPGAFDDAEDFARHDVIALARARRLRRPAWLDSGDEDPFAAAS
                     QVLAQTLGVEKHTWAGGHEADYWHAHYDEYLRFYARALERC"
     gene            complement(1425..2480)
                     /locus_tag="VF517_14040"
     CDS             complement(1425..2480)
                     /locus_tag="VF517_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054108.1"
                     /translation="MAMAYGSDISVYVEDQALDGGASPSPAPWWLSPDIDIPAHPGLA
                     VQGTNQVKIRVHVQDEPVVSELIVAEVYVGDPSLVLSPSSGTVRIDPGNLRFRTLAIQ
                     GPEPVATTAGGEATFDWTPSSTAGAVNGPGHRCLVLRAFPESVTPPTGSFDVPNEQHE
                     AQRNIMILTTTTNPPPSGGGGSGGGGSGAPGDPLDPDRLTGLWRKSILTRALGKKGPR
                     IVVWAFDPAPGKQIEAMVRAYLGKRRKFAGFSRAAPGEVTLYPGRSGREVEPTELLKG
                     SFAGSAGLGKGLFAGDRLVAAAELEASPKAATNLVLGFDHSNIRKYSATVLHGAQWDA
                     DGRPEGGLTVVALAPNP"
     gene            complement(2558..2797)
                     /locus_tag="VF517_14045"
     CDS             complement(2558..2797)
                     /locus_tag="VF517_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054109.1"
                     /translation="MSRLTVSWKPSSSGFRHAVYVMVGDGRRVAEVLDAKRRSFTLKR
                     VSSKVGAGAKVLGLTASNGKGPSASRSIKARKRSS"
     gene            3038..3910
                     /locus_tag="VF517_14050"
     CDS             3038..3910
                     /locus_tag="VF517_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054110.1"
                     /translation="MQGRLSAFTAAFIAAAVFGTFAASAAAQLKQDPIMNSTQPVASN
                     VYFQQTAAQLDNLEANGERLVDFEPDGTDASGDTLFTVATVVPSGPAWEGFNFSSSPA
                     PAGTALPSTDGSVRPFDLEYYLRNSSGDPTADPTPRAGYLGVENTGPFERKWRVLRRR
                     TPGTLKDWVIKNKMRIIDLDSYRGGSASNALVIQNTGSIARKWWFYVNVTTSYINARA
                     KEKKAAITDLEPLRNGRYSVVMERSSLRPYYRVNKTAAQIVDFAVQKNDRIADIDPEP
                     DAPGKYSAVFIPNG"
     gene            complement(3919..3992)
                     /locus_tag="VF517_14055"
     tRNA            complement(3919..3992)
                     /locus_tag="VF517_14055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3956..3958),aa:Val,seq:tac)
     gene            4021..4395
                     /locus_tag="VF517_14060"
     CDS             4021..4395
                     /locus_tag="VF517_14060"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8054111.1"
                     /translation="MQIARLSEVEAFTTLDGSTIREVAGPAWTAARNQSLAEATVPPG
                     SDTAAHYHRTTEELYFFTAGAGRMRLGEDEREVRAGDCVVIPPGVEHKLLNPGGEPLV
                     LLCCCAPPYSDEDTVITEPDAS"
     gene            4405..4749
                     /locus_tag="VF517_14065"
     CDS             4405..4749
                     /locus_tag="VF517_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054112.1"
                     /translation="MDSKRFWRAAAVQGVLVGGLFVVLALALDKEFFEDNGAFVGPLI
                     WIGCSLITGRVLHLPVTFTLFCALAGAVAGIVVEFAAPHGAAIAAAIGVFAASASGYD
                     EGAGSAGQASTP"
     gene            complement(4731..5096)
                     /locus_tag="VF517_14070"
     CDS             complement(4731..5096)
                     /locus_tag="VF517_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054113.1"
                     /translation="MADVTQVTVAQVVHRAAVLCDPEGVDDACTQLILAYEDDDRPAL
                     GLSDTLGEELRSTVAGLDPEHDSAGAEVAAAVATFLATKPHGGNDDEGTIREAARIAW
                     GQSPPPNVSDWLDAQGVEA"
     gene            5163..>7069
                     /locus_tag="VF517_14075"
     CDS             5163..>7069
                     /locus_tag="VF517_14075"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX8054114.1"
                     /translation="MKALVLALLALVLAAPAAGAATHELDAGPLTVRVTDEPFAIELV
                     DEADGDVLRTVAAPGVAASDRRARYGPLGFSFDLRQPVVSNAYFGYYVAAEAETVWFH
                     ATRVTAAHPAAGRLELDLATDDPLGHRLVLTIEALPQGGARLDSRVEGPLAERASLSG
                     AAFEHADGERYLGFGERSNAADQTGNRVISWAEEGPFSAGNYEDELNVVLPGFVFPTG
                     PTTTNFPIPWLVSTRGLGVLIDQTERSYFNLLNERGDAWLAEVESPRLRLSVFAGPRP
                     ADVVRRYSDYAGRQPRPAPWIFGPWFQPTLEKEPLELAKRFRADNVPVSVAQTYTHYL
                     PCGVHRGNEQAERERVADYHRLGFKITTYFNPHVCLDYQPVYDDAARRGLFVKNQAGQ
                     PYVLSNPFTADEQVSEVDFTHPEGPAFYGSLLDEALAAGYDGWMEDFGEYTPADSVLH
                     NGQTGETMHNRYPVEYHCASTRHSRAKAPAVFIRSGFHGVQSCARVVWGGDPTEDWSC
                     TDGICAAVHQLLSTGLSGIAYQGSDIGGFHAIVNGRTTDELNARWIQVGAISGVMRTQ
                     ANGYSVRYSREDRSQVWHEAVYPIWRRWARLRTRLYPYIAGASAQYQASGMPIARHLS
                     LAFPEDRTAAGR"
BASE COUNT          992 a         2594 c         2474 g         1009 t
ORIGIN      
        1 cgccgacgcg gccgggctga ccaacgtgac cacacgcgag ctcgacctcg agtcgatcga
       61 cgaggccgcc gacgcctacg acgcggtgct ctgccgcgag gggctgatgc tcacgagcga
      121 cccggcccga gcgagcgccg agatcgcgcg cgtcctgcgc ccgtccggcc gcttcgcgct
      181 ctcggtctgg ggaccgcgcg agcgcaaccc gtggctcggg ctgctcctcg actccgccgg
      241 cgccgtgctc ggcgtgccgg tgccgccgcc cggcgtgccg ggaccgttct ctctctcgga
      301 ccgcgaccgg ctcgcggccg tgctggccgg cgcggggctt gccgaggtgc aggtcgacga
      361 ggcgtcggtc ccgcggagcg cggggtcgtt cgaggagtgg tggcagcgca ccgtggcgct
      421 ggcgggcccg ctcgcgaaga tgctcgagtc gctgccgccg gagacgcgcg acgcgatcag
      481 gcagcgcgcc gaggaggcgg tccggccgtt cgcgacggcg gacggcgggc tggagttccc
      541 gggcgtggcg ctggtggcgt cggggcgcgc cgcctgagcg tcagcagcgc tcgagcgcgc
      601 gggcgtagaa gcgcaggtac tcgtcgtagt gggcgtgcca gtagtcggcc tcgtggccgc
      661 cggcccaggt gtgcttctcg acgccgagcg tttgcgcaag cacctgggag gcggcggcga
      721 aggggtcctc gtcgccgctg tcgagccagg ccggtcgccg caggcgccgt gcacgagcga
      781 gcgcgatcac gtcgtggcga gcgaagtcct cagcgtcgtc gaacgcgccc ggagccgttt
      841 cgcccgcgct cgcccagatc gcgggcgagt ggccgcccac cgcgcagaag cgcgagggcg
      901 agtgcagagc gatgtcgaaa gcgccgaagc cgcccatcga gatcccaccg atcgccaccc
      961 ggcgtggatc ggcccggagc cggcggaccg cctgtggaat cacctcgcgg accacgtaac
     1021 gaccccacgc ccccccgcgg cggtcgtgcc agtaggagtg gtcgccgccg tcggggaaga
     1081 cgaccgccgg cgcgcgcggg ccgagcgcgc ggagcgcgtc caggaaagcg ccgttcacgt
     1141 tgctctcggc accgcccttg ccacggccgt gcaggaagac gagcagcggc ggccgccgcc
     1201 gcgagtccgg cggagtgaca ccgacctgac tcagagtccg cccggcgtac cggctgtcga
     1261 tctcgtagcg gacgacactc gtgccgtgcg cttcgggatc cccggtccgg acctcgccgc
     1321 caccgccgca ccccgccgcg aacaggcccg cgacgcacac gacgacgggc gcgtgcgatc
     1381 cgagtctccg tctgcgcccc ttcagggtgt tggcggcccg cacatcatgg attcggcgcg
     1441 agggcgacga ccgtgagtcc gccctcgggg cgaccgtcgg cgtcccactg ggccccgtgg
     1501 aggacggtcg cgctgtactt gcggatgttc gagtggtcga atccgagcac gagattggtc
     1561 gcggccttcg gactcgcctc gagctcggcc gccgccacga ggcgatcccc tgcgaacagc
     1621 cccttcccga gacccgcgct tccggcgaag gagcccttca gcagctcggt cggctcgacc
     1681 tccctgccac tgcgcccggg gtacagggtc acctcacccg gcgccgccct ggaaaacccc
     1741 gcgaacttgc ggcgcttgcc caggtacgcg cgcaccattg cctcgatctg cttgccgggc
     1801 gccgggtcga aggcccagac gacgatccgc ggacccttct tgcccagcgc gcgtgtgagg
     1861 atggacttcc gccagaggcc ggtcagccgg tccgggtcga gcggatcgcc gggagcgccg
     1921 gagcctccgc cgccggagcc tcccccgccg gagggcggcg ggttggtcgt cgtcgtcagg
     1981 atcatgatgt tgcgctgagc ctcgtgctgc tcgttcggca cgtcgaagct cccggtcggc
     2041 ggcgtgacgc tctccgggaa cgcgcgcagc acgaggcagc gatggccggg gccgttcacc
     2101 gcgccagccg tcgacgacgg ggtccagtcg aaggtcgcct cgccgccggc ggtggtcgcg
     2161 acaggctcgg gcccctgtat ggcgagggtg cggaaacgca ggttgcccgg atcgatccgg
     2221 accgtcccgc tcgacgggct gagaaccagg ctcgggtcgc ccacatagac ctcggcgacg
     2281 atcagctcgg acacgaccgg ttcgtcctgg acgtgaacgc ggatcttgac ctgattcgtc
     2341 ccctggaccg cgaggccggg atgcgccgga atgtcgatgt cggggctcag ccaccacggc
     2401 gccgggcttg ggctcgcgcc cccgtccagg gcctgatcct caacgtaaac cgagatgtcg
     2461 ctgccatacg ccattgccac cgctccagtt agcttgcgcg cgagcgtatg gacgtaggac
     2521 ggccgctgtc aaggcgccag ccgtcccgcg ctcttagcta gctggagcgc ttcctcgcct
     2581 tgatcgagcg cgacgcgctc ggccccttgc cgttcgaggc ggtgaggccg agcaccttcg
     2641 cccccgcgcc gaccttgctc gagacgcgct tcagggtgaa cgagcgccgt ttggcgtcga
     2701 gcacttcagc cacccgccgg ccgtcgccga ccatcacgta gaccgcgtgg cggaagccgg
     2761 acgagctcgg cttccagctc accgtcagcc gcgacacctt ccgccgggtg gcactcgccg
     2821 cacaggtcct cggtcgtgaa gatcgcgacc ttgtcgcccg agtgcgcccc gcccggcgcc
     2881 gacttgacca gcggctcgtc ggtcgtgccg gggttggaga cgcgtcctcg ggcgcccgct
     2941 taaggcgcca caaagctctt ggcgccaagg atcctgtcgc cgcgggtaca gtccgccgcc
     3001 gttcgcagtt aaacggacag gggacagggg gcagggcatg caggggcgac tctcggcgtt
     3061 cacggctgcg ttcatcgcgg ctgcggtatt cggcaccttc gcggcctctg ccgcggcgca
     3121 gctcaagcag gatccgatca tgaactcgac ccagccggtc gcctcgaacg tgtacttcca
     3181 gcagacggcc gcgcaactcg acaacctcga ggccaacggg gagcggctgg tcgatttcga
     3241 gcccgacggc accgacgcga gcggcgacac gctgttcacg gtggcgaccg tcgtaccgtc
     3301 cggcccagcg tgggaagggt tcaacttcag ctcgtccccc gcacccgcgg gcacggccct
     3361 cccgagcacg gatgggagcg ttcgcccctt cgacctggag tactacctgc gcaactcctc
     3421 gggcgatccg accgcggatc ccaccccccg ggcgggctac ctcggcgtgg agaacaccgg
     3481 tccgttcgag cgcaagtggc gggtgctgcg ccgccggacc ccagggacgc tgaaggactg
     3541 ggtgatcaag aacaagatga ggatcatcga cctggactcc taccgcggcg gctccgcttc
     3601 gaacgccctg gtcatccaga acaccggctc gatcgcccgc aagtggtggt tctacgtgaa
     3661 cgtgacgacg tcgtacatca acgcgcgggc gaaggagaag aaggcggcga tcaccgacct
     3721 cgagccactg aggaatgggc gctacagcgt cgtgatggag cgctcgagcc tcaggccgta
     3781 ctaccgcgtg aacaagacgg cggcccagat cgtggacttc gcggtccaga agaacgaccg
     3841 catcgccgac atcgatcccg agccggacgc gcccgggaag tactcggcgg tgttcattcc
     3901 gaacggctga gccgctcatg ggcggtactg gactcgaacc agtgaccccc tgcttgtaag
     3961 gcaggtgctc tagccagctg agctaaccgc ccgggccggt cgagtctaga gtccggcgcg
     4021 atgcagatcg cgaggctcag cgaggtcgag gcgttcacca cgctcgacgg gtccaccatc
     4081 cgcgaggtgg ccggccccgc ctggacggcg gcccgcaacc agagcctcgc ggaggccacc
     4141 gtgccgcccg gctcggacac cgccgcgcac taccaccgca ccaccgagga gctctacttc
     4201 ttcaccgcgg gcgcgggccg catgaggctc ggcgaggacg agcgcgaggt gcgcgccggc
     4261 gactgcgtgg tgatcccgcc gggcgtggag cacaagctgc tcaatccagg cggcgagccg
     4321 ctggtcctgc tgtgctgctg cgcgccgccg tacagcgacg aggacacggt catcaccgag
     4381 ccggacgcct cgtaggattc cgccgtggat tccaagcggt tctggcgcgc ggcggccgtg
     4441 cagggggtgc tcgtcggcgg cctcttcgtg gtgctcgccc tcgccctcga caaggagttc
     4501 ttcgaggaca acggcgcgtt cgtcggtccg ctgatctgga tcggatgctc gctgatcacc
     4561 ggccgcgtcc tgcacctccc ggtgacgttc acgctcttct gcgccctcgc gggggccgtg
     4621 gccgggatcg tcgtcgagtt cgccgcaccg cacggggccg cgatcgcggc cgccatcggc
     4681 gtcttcgcgg cgtcagcctc gggctacgac gagggggccg gctcggccgg tcaggcctcg
     4741 acgccctgag cgtcgagcca gtcgctcacg ttcggcgggg gcgactggcc ccaggcgatg
     4801 cgggcggcct cgcggatcgt gccctcgtcg tcgttgccgc cgtgcggctt cgttgccagg
     4861 aacgtggcca cggcggcggc cacctcggcc cccgcgctgt cgtgctcggg gtcgaggccc
     4921 gccacggttg agcgcagctc ctcccccagg gtgtcgctca ggccgagtgc cggccggtcg
     4981 tcatcctcgt aggcgaggat gagctgggtg caggcgtcgt cgacgccctc ggggtcgcag
     5041 agcaccgccg cgcgatgcac cacctgggcc acggtcacct gagtcacgtc cgccatgcca
     5101 ccggtagcta cccgggcggc acccggcacg aaacctgcgc gggtacgcga agatcgcgcg
     5161 ccttgaaggc tctcgtccta gccctcctcg cgctcgtcct cgccgcgccg gccgccggcg
     5221 ccgccaccca cgagctcgat gcgggtccgc tgaccgtgcg cgtgaccgac gagccgttcg
     5281 cgatcgagct ggtggacgag gccgacggcg acgtgctgcg cacggtcgct gcccccggcg
     5341 tcgcggcgag tgaccgccgc gcgcgctacg gcccgctcgg cttctcgttc gacctgcgcc
     5401 agccggtcgt gagcaacgcc tacttcggct actacgtcgc ggccgaggct gagacggtct
     5461 ggttccatgc cacccgcgtg accgcggccc accccgccgc cgggcggctc gagctcgacc
     5521 tcgccaccga cgacccgctc ggccaccgcc tcgtcctcac gatcgaggcc ctgccgcagg
     5581 gcggggcgcg gctcgactcg cgcgtcgagg gcccgctcgc cgagcgcgcc tcgctgtccg
     5641 gcgccgcctt cgagcacgcc gacggcgagc gctacctcgg gttcggcgag cgctccaacg
     5701 ccgcggacca gaccggcaac cgcgtgatct cgtgggccga ggagggaccg ttctccgccg
     5761 gcaactacga ggacgagctg aacgtggtcc tgccgggctt cgtgttcccg accggcccga
     5821 ccaccaccaa cttcccgatt ccgtggctcg tctccacccg cgggctcggc gtgctgatcg
     5881 accagaccga gcgctcgtac ttcaacctgc tgaacgagcg cggggacgcg tggctggccg
     5941 aggtcgagtc gccgcggctg cggctgtcgg tgttcgcggg cccgcggccc gccgacgtgg
     6001 tgcgccgtta ctcggactac gcgggccgcc agccccgtcc cgcgccgtgg atcttcggcc
     6061 cgtggttcca gccgacgctg gagaaggagc cgctcgagct ggcgaagcgc ttccgcgcgg
     6121 acaacgtgcc ggtgtcggtg gctcagacct acacccacta cctcccctgc ggggtccacc
     6181 gcggcaacga acaggccgag cgcgagcgcg tggccgatta ccaccggctc ggcttcaaga
     6241 tcacgaccta cttcaaccca cacgtctgcc tcgactacca gccggtctac gacgacgccg
     6301 cgcggcgcgg cctgttcgtg aagaaccagg cgggccagcc gtacgtgctg tcgaacccgt
     6361 tcaccgccga cgagcaggtc tcggaggtcg acttcaccca ccccgagggg ccggcgttct
     6421 acgggagcct cctcgacgag gcactcgccg ccggctacga cggctggatg gaggacttcg
     6481 gcgagtacac gccggccgac tccgtcctcc acaacggaca gaccggggag acgatgcaca
     6541 accgctaccc ggtcgagtac cactgcgcct ccacgcgcca ctcgcgtgcg aaggctccgg
     6601 cggtgttcat tcgctccggg ttccacgggg tgcagtcgtg cgcgcgcgtg gtctggggcg
     6661 gcgacccgac cgaggactgg agctgcaccg acggcatctg cgcggccgtg caccagctcc
     6721 tctccaccgg gctctccgga atcgcctacc agggctccga catcggcggc ttccacgcga
     6781 tcgtgaacgg gcgcaccacc gacgagctga acgcgcgctg gatccaggtc ggcgcgatca
     6841 gcggcgtgat gcgcacgcag gcgaacggct acagcgtgcg ctactcgcgt gaggaccgct
     6901 cgcaggtgtg gcacgaggcc gtctacccga tctggcgccg ctgggcgcgg ctgcgcacgc
     6961 ggctctaccc ctacatcgcc ggagcctcgg cgcagtacca ggcgagcggg atgccgatcg
     7021 cccgccacct gtcgctcgcg ttcccggagg accggaccgc cgccgggcg
//
LOCUS       DASVPL010000257        14326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_30082, whole genome shotgun sequence.
ACCESSION   DASVPL010000257 DASVPL010000000
VERSION     DASVPL010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14326
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30082"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..850)
                     /locus_tag="VF517_14080"
     CDS             complement(110..850)
                     /locus_tag="VF517_14080"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX8054115.1"
                     /translation="MDLLLLFAAGSATALATGLGALPVGWLVRRARDVRPAMWGVTVG
                     LMGVASIVGLLLPALDEGSTASVVAGLAVGVVFLLVAKRLIRVHDVGYHGLRGAGIRR
                     AVLVFLVLLVHSLPEGLAIGTAYASDTAGLSLFVILAIALQNVPEGTSVAMPMEAEGF
                     STRRQFWAAVLTSVPQPLGAVAAFLAVEQVESLLPFSFGFAAGAMLAIVAIELIPEAF
                     ARGHRGLGAAGTAVGAALMLALAAALGV"
     gene            906..1373
                     /locus_tag="VF517_14085"
     CDS             906..1373
                     /locus_tag="VF517_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8054116.1"
                     /translation="MVTKRIGTEGRREATALDRENLGFRLAKASQRWNELLAERFRRD
                     GFPEVRPSYGSILVPLFEEDGLRMGLLAERAGLSKQTMTTMVRLLERDGLVTRTRDPD
                     DGRAWRVSLTRRSHAFRPVAERALEELHALVGERLGADEVARFQRFLEEVQSL"
     gene            1370..1762
                     /locus_tag="VF517_14090"
     CDS             1370..1762
                     /locus_tag="VF517_14090"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8054117.1"
                     /translation="MRSRTVTAVFTAPPERVFGYMSDIENLPRWATEFARELKVVDGR
                     HVVVNGLGEFVFEIRADADTGVVDMYAGPTPDAMGVFPTRVVGLPGGGSAYTFTMFQA
                     PDMPDELFDSQYESLRREFENLEREFAA"
     gene            1795..2556
                     /locus_tag="VF517_14095"
     CDS             1795..2556
                     /locus_tag="VF517_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054118.1"
                     /translation="MEYRALRILLALLAVVAVLVIGLGDSGFDPACLNEYMEPGSDLK
                     RYCAGERPADSTWLALALVVVVAGAVLASVLPNGWVLLGATCVALLAAQAARPEAPTG
                     PGAIDGPERVESQVPPPAPVVRRPVPGENGLPPDQAAAARAQSDAQETSRYVAMCIRG
                     GERPRDCADPDVRNDGLSPEADLDLEVYDRYRWRIRTRTRGVRPAHTYTMRVDLRNDR
                     IVHTCAPRGTIVCIRRGRRVEELRPPGSGAQLVEP"
     gene            2706..3014
                     /locus_tag="VF517_14100"
     CDS             2706..3014
                     /locus_tag="VF517_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054119.1"
                     /translation="MTDSIAGIPAGRLLLAIVAGTILFLGALSISFTQAADADWVCSR
                     TGAKPWEHFDHWFLYIDDTEHRYVSGHTHSNGEHHHIWDTYKDYGFTYVGQTHNNCGV
                     "
     gene            complement(3036..3446)
                     /gene="arfB"
                     /locus_tag="VF517_14105"
     CDS             complement(3036..3446)
                     /gene="arfB"
                     /locus_tag="VF517_14105"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX8054120.1"
                     /translation="MEDPLRVGRGVAIPLAEIELRASRSSGPGGQHANVTASRIEAVF
                     DVEGSSALSDEQKRRVMARVGPRLTAVAQDSRSQARNRELALERLRGRLAAALTVPRS
                     RRATKPSRGARERRLDVKRRRGETKRGRRRPDAD"
     gene            complement(3471..4298)
                     /locus_tag="VF517_14110"
     CDS             complement(3471..4298)
                     /locus_tag="VF517_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8054121.1"
                     /translation="MGVLDGKSAIVTGSARGIGRATAELLAEQGAQVLINDLDGDVAE
                     QAAGEIGGETTVFAGDLTADGVPDQLVQKAVDEFGKIDIVVNNAGYTWDGPIHKMTDE
                     QYQAMLDIHTVVPFKILRAAAPHLREPAKKEKEAGEEVFRKVVNISSVSGTMGNAGQA
                     NYSSGKSGVVGLTKTLAKEWGQFKINVNAVAFGFVETRLTAAKEEAGTFQKEGKEIQL
                     GIPEQLRQMAAMIIPLGRPATPEEAAGGVFFLCSPWSNYVHGQVLNVTGGQFSGMYI"
     gene            4402..6474
                     /locus_tag="VF517_14115"
     CDS             4402..6474
                     /locus_tag="VF517_14115"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX8054122.1"
                     /translation="MALALLLPASAAAAPPASCPGDAIKPTRAITGEFGTEIEGSYVM
                     VPFDVPAGTTQVRVKYCFDQPESPTNAQLRHTLDLGLYEPRERTGVPWGLSEFRGWGG
                     SSHPDVTLTAEGFSSEADRRKHVPGKTTRGFRPGPIEPGQWAAELGVAAVASQDEGDS
                     DGKVGWRVEVELRSDPAFADEPYKPAPYDTRPARSEPGWYAGDMHVHAEHSALGDALM
                     SEVFGFAFGAPPDGAGLDFITLTDYVSGAQWDEIGRHQASYPGKLIMRSSETITYRGH
                     TNAHATTSNVDYRTGPIYARAPNGALTLFTAARTPQHIFDRVHATGGFTQVNHPTIFP
                     SEVPAFAGFCRGCPWDYSDSETDWRKVDAYEVHTGPAGLGQPAGNEPGPNPFTPAAIE
                     EFDRLRREGYRITGVAVSDSHNAGRTPGGVTQSPIGEGTTVVYADELSEEGIQRGVKA
                     GHAYAKLFGHESPDVRLTGRSGDQTAMMGDRLGGETASFEAQVIGGVATALPRTLVVL
                     RDGTPFLTVPVVPVGSTFSFSFDSIGPGDYRIQVMRGSAIDALSNPITLGPRAVPQPP
                     PGAPPGQGPGAGPPSGGGGGEGSGSDPRRPAGPTAMRLYPKPVRVRAGRSVRFRFKVL
                     TADATRARVAGALVRFGGRKARTDENGVAHITVRHRRTGRKRASVSKPGLGKAVAKLR
                     VLRAKSRR"
     gene            complement(6471..6863)
                     /locus_tag="VF517_14120"
     CDS             complement(6471..6863)
                     /locus_tag="VF517_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8054123.1"
                     /translation="MPSRNSELVRVAFDCFRRDDFESARELIHPEVRWYSSGAFPGFD
                     PLYVGHERVREWWQTLQEPWESFTIDIEQEVERGNRVILAVRFRAVGKESGVPVDLPF
                     GQVFEFEDGLVTVFRPFPSWEEALAAAS"
     gene            complement(6866..7720)
                     /locus_tag="VF517_14125"
     CDS             complement(6866..7720)
                     /locus_tag="VF517_14125"
                     /inference="COORDINATES: protein motif:HMM:NF004826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX8054124.1"
                     /translation="MAADVSHAVLITGCSTGIGRATAKYLSERGLTVYATARKIDSIQ
                     DLEEHGCRLLQLDVTDEESMGRAVAAVEEQEGAVGALVNNAGYSLSGAIESVSMEEVR
                     RQFETNVLGLIRMCQLALPGMRRQHHGRIVNVSSMGGKLTFPGGGIYHSTKHAVEGLS
                     DVLRFEVRGFGIDVSIIEPGLIKTEFGNAAVGALGRATEAHDGPYDEFNNVVAATTHG
                     AYEGPGARLAAPPESVARAIHKAITRRRPKPRYVITAGARVMLGVHAVVPDRGWDAMM
                     ASRFPRPR"
     gene            complement(7731..9815)
                     /locus_tag="VF517_14130"
     CDS             complement(7731..9815)
                     /locus_tag="VF517_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054125.1"
                     /translation="MAALAVLALGAGSSSAHEELRGDSTVDHTVTGGDPEGGFQFLRL
                     GPGEPYAVRNDLAAPQGGRDARRRSLAYFGQITDLQLADEESPARVEFADVDPSGTAT
                     AAYRPQEALIPHLVDWAIRQMNLFQTSPVPQGNGEHASMLATLMTGDLADNQQLNETE
                     WVRTLLEGGLLDPNSGTSDLSGTSCPPGTPLDDPRNYTGVQDRDDTPTDDPHFYDPDE
                     PTGEYTGWPKYAGLLDQAQKPFVAEGLKMPSYVLFGNHDGLAQGNQSTTAPIERIATG
                     CVKPLASTGAVTGMDPMALLTPASVMLVPPDQRRQFVDKLQFKKIFDTTRQSDDHGFA
                     LVDKAELDASGGAASYYDWSPAPGIRFVALDTLSEGGVTSQSSNGNVDEPQFNWFEGV
                     LKEATANDELVVVFAHHARSSLTANVQDEATGPCPPDDDHGHSANPGCDRDPRVSNVK
                     LGDDVAALMHRYPHAVAYVAGHSHENRVAPQKSEDGGTAYWEIKSPAVADWPPQDRVI
                     EVMDNCDGTLSIFGTMLDTANRVGAPAPGTSAAGFDSETLASIGRTLTYNDPQLGPGS
                     NAQGKPVDRNVELLIKDPRKLPPACAGANGGGGGGGNGGTAGSTPKIKLSVKPRKVLG
                     GRRTCFRFRATSNGRVVPGAKIGFAGRRANTGRTGKARICRRIYRSKRAYAKRKGFTT
                     GRAKVKVLRRKR"
     gene            complement(9953..11410)
                     /locus_tag="VF517_14135"
     CDS             complement(9953..11410)
                     /locus_tag="VF517_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054126.1"
                     /translation="MVAVLVCGALVAPAGAAPTRVTSATNEVMGLELAGSRLFYGELS
                     GKGLLVTLTDPNNPKRRLYTASTPGSTSDDETGEYSSARSALAASADQLAFAQSTASG
                     SAKYQQGVSSLKVLGGPPIGDLFELESCGHSDPYGPASASVDVDGPRIASTDCSGQIV
                     IRDYTAPAQPTVTRVSPGQGLAAATIAVAGRYVAYNAYGIGPSAPSPTVTVVHDWVAN
                     TKLYEVPRQESFDLQADGKLASVKATPNATDCADHEAQWFSAAEPTAHVLPVRPCTTD
                     VHIVGDRVAVVTNPEDSPSSQRLLTLAGTDGSVNYVARLGTRGMAGADHDFDGQRVAY
                     SITNCAGNRDLFTESATAPQPRDEEQACPVLIRASTAGLGPSERTIPIVIECSRGCNA
                     LAIVRMRLGGKLVIVGSKRIRLTPDDPCTGGAQRYRLKLASPARREVRRRGSVKADLI
                     VSTPDRAGVDRTTKRKLTLRAAKHNASATGSDCSS"
     gene            11564..13099
                     /locus_tag="VF517_14140"
     CDS             11564..13099
                     /locus_tag="VF517_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8054127.1"
                     /translation="MTQQSAPQRSGAGAPSAVHELKTLVLSRHPGILIETTEPERAAL
                     SLADVARDLRLNRLQWSVTTGLRHAFDGGRAAYESQDPAGVLAAIAGLQLEALYELHD
                     FTPYLAKPEVARAFRELLDKFSRPDRLSTVVLVESAAKLPPELDPYVVRYDLRFPSRD
                     EYRVALETVVESLKQNGRATVEIAPDDYDEFCGALTGLTLNQARQAIARAAIEDGFLT
                     RDDLSRLAEFKARALQEDGLLEYFPPADNRYELGGFSNLTRWLERARVGFSPAAAELG
                     LQPPKGILLVGVQGCGKSLAAKVIAREWRLPLLKLDAGRLFDKFIGETERNFRKAIAV
                     AEATAPAVLWIDELEKAIAPSGGGGDTDGGLSRRLFGSFLTWLQEKKAPVFVVATAND
                     LSLLPPELLRKGRFDEIFFVDLPNEQERAEVFRIHLGLRKQDAAAFDFAALVAASEGF
                     SGAEIEQAVIASLLGALHDGVPLTTEHVVAELGSTVPLSVTRREDVAELRASAEGRFV
                     PVR"
     gene            13197..13838
                     /locus_tag="VF517_14145"
     CDS             13197..13838
                     /locus_tag="VF517_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054128.1"
                     /translation="MAWRGVGLLFVVAMLVPATAGASHRPSACERLKGKDLAPHAKVK
                     LVERANADDGKDLLGCVLPAGRVRRVASSADYYTSGENYSIRRIARTVLLMDSSSDSQ
                     YGSARSTWVYDIRTGRSYGIASSSYLLGEPASDPSDTTATTAFVNKAGQAVAAIVPDG
                     TASLTIAGFSAKGKRADLDSGTPEELPAPSLGLNYSTAYWTHSGEPRSAELPG"
BASE COUNT         1983 a         5010 c         5206 g         2127 t
ORIGIN      
        1 tcacgctcga cggcgcgcgc gacaactggt tcgggccatg gccgccgccg gggatgaccg
       61 gcgagggcgg caagccgctg gccgaggagc ggcggccgag gccgccgggc tagaccccga
      121 gcgccgccgc cagcgcgagc atcagcgccg cgccgaccgc cgtgcccgcc gcgccgagcc
      181 cccggtgccc gcgcgcgaac gcctccggga tcagctcgat cgccacgatc gcgagcatcg
      241 cgcccgcggc gaagccgaac gagaacggca gcagcgattc gacctgctcg accgccagga
      301 aggccgccac ggcaccgagc ggctgcggca cgctcgtgag cacggccgcc cagaactggc
      361 gcctggtcga gaagccctcg gcctccatcg gcatcgccac gctggtcccc tcgggaacgt
      421 tctggagcgc gatcgcgagg atcacgaaca ggctgagccc ggcggtgtcc gacgcgtaag
      481 ccgtgccgat cgccagcccc tccgggaggc tgtgcacgag caacacgagg aacacgagca
      541 ccgcgcggcg gatgcccgcg ccgcgcaggc cgtggtagcc gacgtcgtgc acgcggatca
      601 ggcgcttggc gaccaggagg aagacgacgc cgacggcgag cccggcgacc acggatgccg
      661 tcgagccctc gtcgagcgcc gggagcagga ggccgacgat cgacgccacg cccatcaggc
      721 cgacggtgac tccccacatg gccgggcgca cgtcgcgcgc gcggcgcacg agccagccga
      781 ccgggagtgc gccgagaccg gtggcgaggg cggtggcgct gccggcggcg aagagcagca
      841 acaggtccaa ggtgcggagc gtatgggtgg tgtcggagcg cgctatttag tacgatatcg
      901 gactaatggt gaccaagcgc atcggcacgg aggggcggag ggaggccacg gcactcgatc
      961 gcgagaacct cggcttccgc ctcgcgaagg cgtcgcagcg ctggaacgag ctcctcgcgg
     1021 agcgattccg gcgcgacggc ttcccggagg tgaggccgag ctacggctcg atcctcgttc
     1081 ctctgttcga ggaggacggc ctgcggatgg gactgctcgc ggagcgggcg ggcctcagca
     1141 agcagacgat gaccacgatg gtccgcctcc tagaacgcga cgggctcgtc acgcgcacgc
     1201 gcgaccccga cgacgggcgc gcatggcggg tgtcgctcac gcggcgctcg cacgcgttcc
     1261 gtcccgtggc cgagcgcgcg ctcgaggagc tccatgcgct cgttggtgag cgcctcggtg
     1321 cggatgaggt cgccaggttc caacgattcc tggaggaggt ccagtcgcta tgaggtcacg
     1381 caccgtaacc gccgtcttca ccgccccgcc ggagcgggtc ttcggctaca tgtccgacat
     1441 cgagaacctg ccgcgctggg cgaccgagtt cgcccgcgag ctgaaggtcg tcgacggccg
     1501 gcacgtggtc gtcaacgggc tcggggagtt cgtgttcgag atccgggcgg acgcggacac
     1561 cggcgtggtg gacatgtacg cgggcccgac gcccgacgcg atgggcgtgt tcccgacgcg
     1621 cgtggtcggg ctgccgggcg gcggcagcgc ctacacgttc acgatgttcc aggcccccga
     1681 catgccggat gagctgttcg actcgcagta cgagtcgctg cgccgggagt tcgagaacct
     1741 ggagcgggag ttcgccgcct aacgggcccg cccgccgcgg tcgatcaccg ccgcatggaa
     1801 tatcgggccc tccggatact gctcgcgctc ttggcggtgg tcgcggtcct cgtgatcggg
     1861 ctgggggact ccggcttcga ccccgcgtgc ctcaacgagt acatggagcc ggggtcggac
     1921 ctgaagcgct actgcgcggg cgagcggccg gccgactcga cgtggctcgc gctcgccctc
     1981 gtggtggtcg tggcgggcgc ggtcctcgcg agcgtcctgc ccaacgggtg ggttttgctc
     2041 ggggccacgt gcgtcgcgct gctggccgcg caggcggcgc ggccggaggc gccgaccggt
     2101 cctggcgcga tcgacggccc ggagcgcgtc gagagccagg tcccaccgcc cgcgccggtc
     2161 gtacgcaggc cggtcccagg cgagaacggc ttaccgccag accaggcggc ggccgcgcgc
     2221 gctcagagcg atgcccagga gacatcccgg tacgtcgcca tgtgcatcag gggcggtgag
     2281 cgtccgaggg actgcgcgga ccccgacgtg cgcaacgacg gtctgagccc ggaggcggac
     2341 ctcgacctcg aggtctacga ccgctatcgg tggcggatcc gcacgcgtac gcgcggcgtc
     2401 cggccggcgc acacgtacac gatgcgcgtg gacctgcgca acgaccggat cgtccacacg
     2461 tgcgcgccgc gggggacgat cgtctgcatc cggcgcggtc gcagggtcga agagctgcga
     2521 ccaccgggaa gcggcgcgca gctcgtcgag ccctgagcgt tggaggagcg cgagcactcg
     2581 cgcgtcagga acctcaccgg gatggggtag ccgcgcactc atcggcggtc atcaccaccg
     2641 catggggggc ttgcttttcc ttgagtgcaa aacaagtgtg aacccaatcc aagggaggcg
     2701 cacgcatgac tgactcaatc gcaggtatcc cggccgggcg gctactgctg gcaatcgtcg
     2761 ccggcacgat cctgttccta ggggcgttgt cgatctcgtt cacccaggcg gcggacgccg
     2821 actgggtctg ctcgcgaacc ggagccaagc cgtgggagca cttcgaccac tggttcctct
     2881 acatcgacga caccgagcac cgctacgtaa gcgggcacac ccactcgaac ggcgagcacc
     2941 accacatctg ggacacgtac aaggactacg gcttcaccta cgtcgggcag acgcacaaca
     3001 actgcggggt ctagggggcc cgtgcggcct ggcgcctagt cggcgtcggg ccgccgccgg
     3061 ccgcgcttgg tctcgccgcg gcggcgcttc acgtctaagc gccgctcgcg cgccccgcgc
     3121 gagggcttgg tcgcccggcg cgagcgcggc accgtcagtg ccgccgcgag ccggccgcgc
     3181 agccgctcga gcgccagctc gcgattgcgc gcctgggagc gcgagtcctg cgccacggcg
     3241 gtcaggcgcg ggcccacccg cgccatcacc cgccgcttct gctcgtcgct gagcgcgctc
     3301 gacccctcga cgtcgaacac ggcctcgatc cgcgaggccg tcacgtttgc gtgctggccg
     3361 cccggaccgg acgaacggct cgcgcgcagc tcgatctccg cgagcgggat cgcgacgccg
     3421 cggccgaccc gcagcgggtc ctccacggcg cgcgctcccg tcccgaggac ctagatgtac
     3481 atgccggaga actggcctcc ggtcacgttc agcacctggc catgcacgta gttcgaccac
     3541 ggcgagcaga ggaagaacac gccgccggcg gcctcctcgg gcgtggccgg gcggccgagc
     3601 ggaatgatca tcgcggccat ctggcggagc tgctccggga tgccgagctg gatctccttg
     3661 ccctccttct ggaaggtgcc cgcctcctcc ttggccgccg tgagccgcgt ctcgacgaac
     3721 ccgaacgcca ccgcgttcac gttgatcttg aactggcccc actccttcgc gagcgtcttg
     3781 gtgagcccga cgacgcccga cttgcccgac gagtagttcg cctgcccggc gttgcccatc
     3841 gtgcccgaga ccgacgagat gttcacgacc ttgcggaaca cctcctcgcc ggcctccttc
     3901 tccttcttgg ccggctcgcg caggtgcggc gcggcggcgc gcaggatctt gaacggcacc
     3961 accgtgtgga tgtcgagcat cgcctggtac tgctcgtcgg tcatcttgtg gatcgggccg
     4021 tcccacgtgt agcccgcgtt gttgacgacg atgtcgatct tgccgaactc gtcgacggcc
     4081 ttctggacga gctggtcggg cacgccgtcg gctgtgaggt cgcccgcgaa gaccgtggtc
     4141 tcgcccccga tctcgcccgc cgcctgctcg gcgacgtcgc cgtcgaggtc gttgatgagg
     4201 acctgcgcgc cctgctcggc gagcagctcg gccgtggcgc ggccgatgcc gcgcgccgag
     4261 cccgtgacga tcgccgactt gccgtcgagt actcccactg gtgcttctcc ttgtccgctt
     4321 tcgaggcgcc cactatgacg cagcgcggca cgcgggggcc ggcggtaccc tcgccatatc
     4381 gggcgcagga tcctcatcgg ggtggctctg gcgctgctgc tgccggcgag cgcggcggcg
     4441 gcgcccccgg cgagctgccc cggcgacgcg atcaagccca cgcgcgcgat cacgggcgag
     4501 ttcgggaccg agatcgaggg cagctacgtg atggtgccgt tcgacgtccc ggccggcacc
     4561 actcaggtgc gcgtcaagta ctgcttcgac cagccggagt cgcccaccaa cgcgcagctc
     4621 aggcacacgc tcgacctggg gctgtacgag ccgcgcgagc ggaccggcgt gccgtggggg
     4681 ctctccgagt tccgcggctg gggaggctcg agccatcccg acgtgacgct caccgccgag
     4741 ggcttctcga gcgaggcgga ccggcgcaag cacgtgcccg gcaagaccac gcgcggcttc
     4801 aggcccggcc cgatcgagcc agggcagtgg gccgcggagc tgggcgtcgc ggccgtcgcc
     4861 tcacaggacg agggcgacag cgacgggaag gtggggtgga gggtcgaggt cgagctgcgc
     4921 tccgacccgg ccttcgcgga cgagccgtac aagccggctc cgtacgacac ccggcccgca
     4981 cgctccgagc cgggctggta cgcgggcgac atgcacgtgc acgccgagca ctcggcgctc
     5041 ggcgacgcgc tgatgagcga ggtcttcggc ttcgcgttcg gagcgccgcc ggacggcgcc
     5101 gggctcgact tcatcaccct caccgactac gtcagcggcg cgcagtggga cgagatcggc
     5161 cgccaccagg cctcctaccc aggcaagctg atcatgcgca gcagcgagac catcacctac
     5221 cgcgggcaca ccaacgcgca cgccacgacc agcaacgtcg actaccggac cgggccgatc
     5281 tacgcgcgcg cgcccaacgg agccctgacc ctgttcaccg ccgcgcgcac gccgcagcac
     5341 atcttcgacc gcgtgcacgc cactggaggc ttcacgcagg tcaaccaccc cacgatcttc
     5401 ccgtccgagg tgcctgcctt cgcggggttc tgccgcggct gcccgtggga ctactcggac
     5461 tcggagacgg actggcgcaa ggtcgacgcg tacgaggtgc acaccgggcc cgccggcctg
     5521 gggcagccgg ccggcaacga gcccggcccg aacccgttca cgcccgccgc gatcgaggag
     5581 ttcgaccgcc tgcgccggga gggctaccgg atcaccggcg tcgccgtgag cgactcgcac
     5641 aacgccggcc gcactccggg cggcgtcacg cagtcgccga tcggcgaggg caccacggtc
     5701 gtctacgcgg acgagctctc cgaggagggg atccagcgcg gggtgaaggc cggccacgcg
     5761 tacgcgaagc tgttcggcca cgagagcccg gacgtacggc tcaccggccg ctccggcgac
     5821 cagaccgcga tgatgggcga ccgcctcggc ggcgagacgg cttcgttcga ggcgcaggtc
     5881 atcggcggcg tcgcgaccgc gctcccgcgc acgctcgtag tgctccgcga cggcacgccg
     5941 ttcctcaccg tgcccgtggt gccggtcggg tcgacgttct cgttctcgtt cgactcgatc
     6001 ggcccgggcg actaccggat ccaggtgatg cgcggctcgg cgatcgacgc gctgtcgaac
     6061 ccgatcacgc tcggtccgcg ggcggtgccg cagccgccgc cgggcgcgcc gcccgggcag
     6121 gggccgggcg ccggaccgcc ctcgggtggc ggcggcggtg agggcagcgg ctccgatccc
     6181 aggcgcccgg ccgggcccac ggcgatgcgc ctctacccga agccggtacg cgtgcgcgcg
     6241 ggacggagcg tgcgcttccg gttcaaggtc ctgaccgccg acgcgacgcg cgcgcgtgtc
     6301 gcgggcgctc tcgtccgctt cggggggagg aaggcgcgga cggacgagaa cggcgtcgcc
     6361 cacatcacgg tccgccaccg caggacgggc cgcaagcgcg ccagcgtgtc gaagcccggc
     6421 ctgggcaagg ccgtggcgaa gctgcgcgtg ctgcgcgcga agtcgcggcg ctagctcgcg
     6481 gccgccagcg cttcctccca cgacgggaag ggcctgaaga cggtcaccag gccgtcctcg
     6541 aactcgaaga cctggccgaa cggaagatcc accggcacgc cgctctcctt gccgaccgcc
     6601 ctgaagcgca cggccaggat cacgcggttg ccgcgctcga cctcctgttc gatgtcgatc
     6661 gtgaacgact cccacggctc ctggagcgtc tgccaccact cgcgcacgcg ctcgtggccg
     6721 acgtagaggg gatcgaagcc ggggaacgcg ccgctcgagt accagcggac ctcggggtgt
     6781 atgagctcgc gagcgctctc gaagtcgtcg cgacggaagc agtcgaacgc gacccggacg
     6841 agttcggagt tgcggctcgg cactgtcagc ggggccgcgg gaagcggctg gccatcatcg
     6901 cgtcccagcc gcggtcgggc acgacggcgt gcacgccgag catcacgcgc gcgcccgccg
     6961 tgatcacgta gcgcggcttc ggccggcgcc gtgtgatcgc cttgtggatc gcgcgcgcca
     7021 ccgactccgg cggcgccgcc agccgggcgc cgggcccctc gtaggcgccg tgcgtggtgg
     7081 cagcgacgac gttgttgaac tcgtcgtacg gaccgtcgtg ggcctcggtc gcgcgcccga
     7141 gggcacccac cgcggcgttg ccgaactccg tcttgatcag ccccggctcg atgatcgaga
     7201 cgtcgatccc gaacccgcgc acctcgaagc gcaacacgtc cgagagcccc tcgaccgcgt
     7261 gcttggtcga gtggtagatc ccgccaccgg ggaaggtgag cttgccgccc atcgagctca
     7321 cgttcacgat ccggccgtga tgctggcgcc gcatgcccgg cagcgcgagc tggcacatgc
     7381 ggatcaggcc gagcacgttg gtctcgaact ggcgccggac ctcctccatc gagaccgact
     7441 cgatcgcgcc cgagaggctg tacccggcgt tgttcacgag cgcgcccacg gcgccctcct
     7501 gctcctcgac cgccgccacc gcgcgcccca tcgactcctc gtcggtcacg tcgagctgga
     7561 gaagccggca gccgtgctcc tcgaggtcct ggatcgagtc gattttgcgg gcggtcgcgt
     7621 agaccgtcag cccgcgttcg gagaggtact tcgcggtggc gcggccgatg ccggtcgagc
     7681 agccggtgat cagtacggcg tgggagacgt cggcggccac gcgtggcacg ctaccgcttc
     7741 cggcgcagga ccttcacctt ggcgcggccg gtcgtgaagc ccttgcgctt ggcgtacgcg
     7801 cgcttggacc ggtagatcct gcggcagatc ctcgccttcc cggtcctgcc ggtgttcgcc
     7861 ctgcgccccg cgaacccgat cttcgcgccc ggcaccacgc ggccgttcga cgtggccctg
     7921 aagcggaagc acgtcctgcg cccgccgagc accttcctcg gcttcaccga cagcttgatc
     7981 ttcggagtcg atccggccgt gccgccgttg ccgccgccac cgccgccgcc gttggccccc
     8041 gcgcaggcgg gcggcagctt ccgcgggtcc ttgatgagca gctcgacgtt gcggtcgacc
     8101 ggcttgccct gcgcgttgct ccccggcccg agctgagggt cgttgtaggt gagcgtgcgg
     8161 ccgatcgacg cgagcgtctc ggagtcgaac ccggcggcgg aggtgcccgg cgccggggcg
     8221 cccacgcggt tcgcggtgtc gagcatcgtc ccgaagatcg agagcgtgcc gtcgcagttg
     8281 tccatcacct cgatcacgcg gtcctgcggc ggccagtcgg ccaccgccgg gctcttgatc
     8341 tcccagtagg cggtgccgcc gtcctcggac ttctgcggcg ccacgcggtt ctcgtgcgag
     8401 tggcccgcca cgtacgcgac cgcgtgcggg tacctgtgca tgagcgccgc cacgtcgtcg
     8461 ccgagcttca cgttcgacac gcgcgggtcg cggtcgcagc ccgggttggc cgagtggccg
     8521 tggtcgtcgt ccggcggaca cggaccggtg gcctcgtcct ggacgttcgc cgtcaggctc
     8581 gagcgcgcgt ggtgcgcgaa caccacgacc agctcgtcgt tggcggtcgc ctccttcagc
     8641 acgccctcga accagttgaa ctgaggctcg tcgacgttgc cgttgctgct ctgcgacgtg
     8701 accccgccct cggagagcgt gtccagcgcg acgaagcgaa tgcccggcgc cggcgaccag
     8761 tcgtagtagg aggcggctcc gccggaggcg tcgagctcgg ccttgtccac cagggcgaac
     8821 ccgtggtcgt cgctctgccg ggtggtgtcg aagatcttct tgaactggag cttgtccacg
     8881 aactgccggc gctggtcggg cgggacgagc atcacggacg cgggggtgag gagcgccatc
     8941 gggtccatgc cggtgacggc accggtggac gcgagcggct taacgcatcc ggtggcgatc
     9001 cgctcgatcg gagcggtcgt gctctggttg ccctgcgcga gcccgtcgtg gttgccgaac
     9061 aggacgtagc tcggcatctt caggccctcg gccacgaatg gcttctgcgc ctggtcgagc
     9121 agcccggcgt acttcggcca gccggtgtac tcgcccgtgg gctcgtcggg gtcgtagaag
     9181 tgggggtcgt cggtcggcgt gtcgtcgcgg tcctgcacgc ccgtgtagtt gcgcgggtcg
     9241 tcgaggggcg tgccgggcgg gcagctcgtc ccgctcaggt cgctggtgcc gctgttcggg
     9301 tcgagcaggc cgccctccag cagggtgcgg acccactctg tctcgttgag ctgctggttg
     9361 tcggcgaggt cgccggtcat cagcgtggcg agcatcgacg cgtgctcgcc gttgccctgc
     9421 gggaccgggc tcgtctggaa caggttcatc tgccggatcg cccaatccac caggtggggg
     9481 atcagggcct cctgcgggcg gtacgccgcg gtggcggtgc ccgaggggtc cacgtcggcg
     9541 aactccacgc gcgcgggcga ctcctcgtcg gccagctgga ggtcggtgat ctggccgaag
     9601 taggcgagcg agcggcggcg ggcgtcgcgg ccgccctgcg gcgcggccag gtcgttgcgc
     9661 accgcgtacg gctccccggg gccgagccgc aggaactgga agcccccctc cgggtcgccg
     9721 ccggtcacgg tgtggtcgac cgtggagtcg ccgcgcagct cctcgtgcgc gctcgagctg
     9781 cccgcgccga gcgcgagcac cgccagggcc gccaccgggg cggccgtacg cctcacccat
     9841 cccacggctg caatgttggc accgccgtct cgcgcgtgcg gagggcgggg gtatcttcgc
     9901 gcgcgcctag cgtgccgcac ctcgccgagc gagcgcgggc gctcgcgggg gcctagctcg
     9961 agcagtcgct cccggtcgcg gacgcgttgt gcttcgccgc gcgcagcgtc agcttgcgct
    10021 tggtcgtccg gtcgacgccg gcgcggtccg gcgtgctcac gatcaggtcc gccttgacgg
    10081 aacctcgccg gcgcacctca cgccgcgcgg ggctcgccag cttcagccgg tagcgctgcg
    10141 cgccgcccgt gcacgggtcg tcgggcgtga gccggatccg cttgctgccg acgatcacga
    10201 gcttgcctcc gaggcgcatg cgcacgatcg cgagcgcgtt gcacccgcgc gagcactcga
    10261 tcacgatcgg gatcgtgcgc tcgctcgggc cgaggccggc cgtgctcgca cggatcagca
    10321 ccgggcacgc ctgctcctcg tcgcgcggct gcggagcggt ggcgctctcg gtgaacaggt
    10381 cgcggttgcc cgcgcagttg gtgatcgagt acgcgacgcg ctggccgtcg aagtcgtgat
    10441 cggcgcccgc catgccgcgg gtgccgagtc gtgccacgta gttcacgctg ccgtcggtgc
    10501 cggcgagcgt gagcaggcgc tgcgatgacg gcgagtcctc ggggttcgtg accaccgcca
    10561 cgcggtcgcc aacgatgtgc acgtccgtgg tgcacgggcg gaccggcagc acgtgcgcgg
    10621 tcggctccgc cgcggagaac cactgggcct cgtggtcggc gcagtcggtg gcgttcggcg
    10681 tcgccttgac gctggcgagc ttgccgtcgg cctggaggtc gaacgactcc tgccgcggga
    10741 cctcgtagag cttcgtgttc gcgacccagt cgtggaccac cgtgaccgtc ggcgacggcg
    10801 cggacggacc gattccgtac gcgttgtagg cgacgtagcg gccggcgacg gcgatggtcg
    10861 ccgcggcgag gccctgcccc gggctcacgc gggtgacggt cggctgggcc ggcgcggtgt
    10921 agtcgcggat gacgatctgc ccggagcagt cggtcgacgc gatgcgcggg ccgtcgacgt
    10981 cgaccgaggc ggaggcgggg ccgtagggat cgctgtgacc gcaggactcg agctcgaaga
    11041 ggtcgccgat cggcggtccg ccgagcacct tgaggctcga gacgccctgc tggtacttgg
    11101 cgctccccga agctgtggac tgggcgaagg cgagctggtc ggcggacgcg gcgagggcgg
    11161 atcgggcgga ggagtactcg cccgtttcgt cgtcggacgt ggagcccggc gtgctcgcgg
    11221 tgtagaggcg gcgcttgggg ttgttcgggt cggtgagcgt caccaggagt cccttgcccg
    11281 agagctcgcc gtagaagagt cgcgagcccg cgagctcgag gcccatgacc tcgttcgtgg
    11341 cgctcgtcac tcgcgtgggc gcggccccgg ccggtgccac gagagccccg cagacgagta
    11401 cagcgaccaa cccgaccagc attccctgcc gcattcgcgt ctccccgatt cgccttggtt
    11461 gctgccgcta accctgcccc tgccgggagg ttgcggcgcg caggctacac gcgcgcgtct
    11521 caagaagcgc cgcgtagttg ccgactcgct ctaaatggag gccatgacgc agcagagcgc
    11581 gccgcagcgc agcggtgccg gtgctccgag cgcggtccac gagctgaaga cgctggtgct
    11641 ctcgcggcat cccgggatcc tcatcgagac caccgagccc gagcgcgccg cgctgtcgct
    11701 ggccgacgtg gcacgcgacc tgcggctcaa ccgccttcag tggtcggtga ccacgggact
    11761 caggcacgcg ttcgacggcg gccgggctgc ttacgagtcg caggacccgg ccggcgttct
    11821 ggctgccatc gccgggctgc agctggaggc gctgtacgag ctgcacgact tcacgccgta
    11881 cctcgccaag ccggaggtgg cgcgagcgtt ccgtgagctg ctcgacaagt tctcccgccc
    11941 cgatcgcctc tcgacggtgg tcctggtgga gtctgccgcc aagctgccgc cggagctcga
    12001 cccgtatgtc gtgcgctacg acctgcggtt cccgagtcgc gacgagtacc gcgtcgcgct
    12061 cgagaccgtc gtggagtcgc tgaagcagaa cggccgcgcg accgtcgaga ttgcccccga
    12121 cgactacgac gagttctgcg gcgcgttgac cgggctgacg ctcaaccagg cgcggcaggc
    12181 catcgctcgt gccgcgatcg aggacgggtt cttgacgcgg gacgaccttt cccgcctggc
    12241 cgagttcaag gcgcgcgcgc tccaggagga cgggctgctc gagtacttcc cgcccgccga
    12301 caaccgctac gagctgggcg ggttctcgaa tttgacgcgc tggctcgagc gggcgcgggt
    12361 gggcttctcg cccgccgccg ccgagctggg gctgcagccg cccaagggga tcctcttggt
    12421 cggggtgcag ggctgcggga agtcgctggc ggcgaaggtg atcgcgcgcg agtggcggct
    12481 gccgctgttg aagctcgacg ccggccggct cttcgacaag ttcatcggcg agaccgagcg
    12541 caacttccgc aaggcgatcg cggtggccga ggccaccgcg cccgcggttc tctggatcga
    12601 cgagctcgag aaggcgatcg cgcccagcgg gggtggggga gataccgacg gcgggctctc
    12661 gcggcgcctg ttcgggtcgt tcttgacctg gctccaggag aagaaggcgc cggtcttcgt
    12721 ggtggcgacc gcgaacgacc tatcgctgct gccgccggag ctgctgcgca aggggcgctt
    12781 cgacgagatc ttcttcgttg atctgcctaa tgagcaggag cgtgcggagg tcttccggat
    12841 tcacctcggg ttgcggaagc aggacgcagc ggcttttgat ttcgccgctc tggttgccgc
    12901 gagcgagggg ttcagcgggg ccgagatcga gcaggcggtg atcgcctcgc tgctgggcgc
    12961 gctgcacgac ggggtgccgc tgacgacgga gcacgtggtt gcggagctgg ggtcgacggt
    13021 gccgctgtcg gtgacgcggc gcgaggatgt ggcggagctg cgggctagcg ccgaggggcg
    13081 gttcgtgccg gtgcggtagg gcctggcggg ttcccgctgg cgcggcggtc gatacgcaac
    13141 aatctcgtcg cggcgtggtt cagacgcgaa cgcgacgaaa ggcgggggac atgcgggtgg
    13201 catggagagg cgtcggtctg ctgttcgttg tggcgatgct cgtgccggcg acggcggggg
    13261 cctcgcacag gccgagcgcc tgcgagcgcc tgaagggcaa ggatctcgcg ccgcacgcga
    13321 aggtgaagct ggtggaacgc gcgaacgccg acgatggcaa ggacctcctc ggctgcgtgc
    13381 tgccggcggg ccgcgtccgc cgcgtggcct cgagcgccga ttactacacg tccggcgaga
    13441 actactcgat ccgccggatc gcgcgcaccg ttctcctgat ggactcctcg tcggacagcc
    13501 agtacggcag cgcccgctcc acctgggtct acgacattcg caccggtcgc tcgtacggca
    13561 tcgcgagcag ctcctatctc ctgggagagc cggccagcga cccgtcggac acgaccgcga
    13621 ccacagcgtt cgtcaacaag gcggggcagg cagttgccgc gatcgttccc gacggcaccg
    13681 cgtcgctgac gatcgccgga ttctcggcga agggaaagcg ggcggacctg gactccggga
    13741 cgccggagga gctcccggcg ccgtcgctcg gtctgaacta ctcgaccgcg tactggaccc
    13801 actcgggcga gccgcggagc gcggagctgc cggggtaggc gcgcgcgccg ctgtgcgaca
    13861 tggaggtctt cgagggtgga aggggatgat cgggcgtgac acctagctct agggcgtagg
    13921 gccgcgctgc gtcttcgttg gatggttcgc ccaggctgcg gggaggtcca tctgcgagac
    13981 gaataacatc gcgatctgcg atgaatgaca tctcgaaatt ttgatgctga acgggcccac
    14041 gaactgccga gcgctctcgg cgagcagctg gcagtgggaa acgaggacta cgagttggtt
    14101 gtcattggcg gatcagctct gcaggcactg ggtcttgtgg atcgcccgac agcgacatcg
    14161 acgtcgtcgc gctgagtctc ggaaccactt tggtcacggc tgagccgctg ccgccgccgc
    14221 tcttggccgc ggctggccga gtggctcgag acttcgcgct gcccgatcgc tggctgaact
    14281 ccggcccgac gagccttctg gatttcgggc ttcccgatgg cttcgt
//
LOCUS       DASVPL010000258         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_267010, whole genome shotgun sequence.
ACCESSION   DASVPL010000258 DASVPL010000000
VERSION     DASVPL010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267010"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VF517_14150"
     CDS             complement(<1..270)
                     /locus_tag="VF517_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054129.1"
                     /translation="MIGYAELTSPDEEAQRQGFRAIRFALQEPPGERGVFDQLVLARG
                     EEGYPKPADEDLHEFVEKVAERWRPHQSKKDGRYLDPVNGIAASGY"
     gene            complement(627..2096)
                     /locus_tag="VF517_14155"
     CDS             complement(627..2096)
                     /locus_tag="VF517_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX8054130.1"
                     /translation="MRSRRSTQSRALLALLALLVLAPSAAAAPQPPFDQHGRWMLDAK
                     GRVVVLHGVNMVNKRPPYAPDALGFGADDVKAIKREGFNTVRLGIIYKGLEAERGRYD
                     DGYLARIVATARLLQKHGIYPLVDFHQDMYNERFNGQGFPDWAVRDDGLPAQPDQGFP
                     ANYLIMPALQRAFDYFFDDVDLQEPYAAAWAHVAQRFRKERGLVGFDIFNEPFPGTRW
                     QSCANPAGCPDLDAKLAAFSKRTFEAIRRVDRRNVLWWEPYLVFNNGAQTSHGDTGDG
                     RAGMSFHMYCLGETPGLAPPGGPPDTGGCGVEDELVLDNARSVSDATGDALLLSEFGA
                     SDDLASIGRVVEEADAEEMSWQYWSWWNRDVCCERPTEGVIRDISKPPTPDNLKQDKL
                     DVLVRPYPQAVAGTPERWSFDRATREFALEYRVKGPDGRRRPTYVPTEVFVPRRHYPK
                     GYTVEVSGARVVSKKNAELLLLRAKLAAGKVSVRVRPRG"
     gene            complement(2068..>2320)
                     /locus_tag="VF517_14160"
     CDS             complement(2068..>2320)
                     /locus_tag="VF517_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054131.1"
                     /translation="TKANGLDDKPLDQLVEDPKVIEAVQAEIDKANDSLARVEQIKKF
                     KILPTEWEPGGEELTPTMKLKRKPIAEKYRDEIEALYAK"
BASE COUNT          290 a          842 c          817 g          371 t
ORIGIN      
        1 gtagccgctc gcggcgatgc cgttcacggg gtcgaggtag cggccgtcct tcttcgactg
       61 gtgcggccgc cagcgctcgg cgaccttctc cacgaactcg tgcaggtcct cgtccgccgg
      121 cttcgggtag ccctcctcgc cgcgcgcgag cacgagctgg tcgaacactc cccgctcgcc
      181 aggcggctcc tggagcgcaa agcggatcgc gcggaagccc tggcgctggg cctcctcgtc
      241 ggggctcgtg agctcggcgt agccgatcat cccgctgccg tatccgctgg cggcgatgcc
      301 gttgatgggg tcgagatagc ggccgtcctt ggcctggtgg ctgcgccagc gctcggctat
      361 ctgctcgacg aagccgtgca gctcctggtc cgccggctcc gggtagtcgt cgccgcgccc
      421 cgcgaagagc caagcgacga gcgcaacgag tgcgacgccg ccggcgagtg cgacgagccg
      481 ccgccgcgcg cggcggcggc gttgctcccg ctgggccgcg cgccggcgct ctctggtcgg
      541 tacctcgagg atcgggtgag gataggaagc ggctgcgccg caaagcgcgc gcgccgccgg
      601 cgtggcgccg acccgccgcc ccgcggctac ccgcgcggcc tcacgcgcac ggagaccttc
      661 ccggccgcca gcttggcgcg caggagcagc agctccgcgt tcttcttcga gaccacgcgg
      721 gcgccgctca cctcgaccgt gtagcccttc gggtagtgcc ggcgcggcac gaagacctcg
      781 gtcggcacgt aggtcggccg ccggcgcccg tccggcccct tcacgcggta ctcgagcgcg
      841 aactcgcgcg tcgcgcggtc gaagctccag cgctccggcg tgcccgccac ggcctgcggg
      901 tacggccgca ccagcacgtc gagcttgtcc tgcttgaggt tgtcgggggt gggcggcttc
      961 gagatgtcgc ggatcacgcc ctcggtgggc cgctcgcagc acacgtcgcg gttccaccac
     1021 gaccagtact gccacgacat ctcctcggcg tcggcctcct ccaccacgcg gccgatcgag
     1081 gcgaggtcgt cgctcgcgcc gaactcgctg agcagcagcg cgtcgccggt cgcatccgac
     1141 acgctgcgcg cgttgtcgag caccagctcg tcctcgaccc cgcagccgcc cgtgtccggc
     1201 ggcccgccgg gcggcgcgag cccgggcgtc tcgcccaggc agtacatgtg gaagctcatc
     1261 ccggcgcggc cgtcgcccgt gtcgccgtgc gaggtctgcg cgccgttgtt gaacacgagg
     1321 tagggctccc accagaggac gttgcgccgg tccacgcggc ggatcgcctc gaacgtccgc
     1381 ttcgagaacg ccgcgagctt cgcgtcgagg tcgggacacc cggccgggtt ggcgcagctc
     1441 tgccagcgtg tgccggggaa cggctcgttg aagatgtcga agccgacgag gccgcgctcc
     1501 ttgcggaagc gctgcgccac gtgcgcccac gccgcggcgt agggctcctg gaggtccacg
     1561 tcgtcgaaga agtagtcgaa cgcgcgctgg agggccggca tgatcaggta gttcgccggg
     1621 aagccctggt ccggctgggc cggcaggccg tcgtcgcgca ccgcccagtc cgggaatccc
     1681 tgcccgttga agcgctcgtt gtacatgtcc tggtggaagt ccacgagcgg atagatgccg
     1741 tgcttctgca gcagccgcgc ggtggcgacg atccgcgcca ggtacccgtc gtcgtagcgg
     1801 ccgcgctcgg cctcgagccc cttgtagatg atcccgagcc ggacggtgtt gaagccctcg
     1861 cgcttgatcg ccttcacgtc gtcggcgccg aacccgagcg cgtccggcgc gtacggcggg
     1921 cgcttgttga ccatgttcac gccgtggagc acgacgacgc ggcccttcgc gtcgagcatc
     1981 cagcggccgt gctggtcgaa cggaggctgc ggcgccgccg cggcggacgg cgccaggacc
     2041 agcagggcga ggagggcgag gagggcccta ctttgcgtag agcgcctcga tctcatccct
     2101 gtacttctcg gcgatcggct tgcgcttgag cttcatcgtc ggcgtcaact cctcaccgcc
     2161 gggctcccac tcggtgggca ggatcttgaa cttcttgatc tgctccacgc gcgcgagcga
     2221 gtcgttggcc ttgtcgatct ccgcctggac ggcctcgatc accttcgggt cctccacgag
     2281 ctggtcgagc ggcttgtcgt cgagcccgtt ggccttcgtc
//
LOCUS       DASVPL010000259         4388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_384743, whole genome shotgun sequence.
ACCESSION   DASVPL010000259 DASVPL010000000
VERSION     DASVPL010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384743"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VF517_14165"
     CDS             complement(<1..477)
                     /locus_tag="VF517_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8054132.1"
                     /translation="MRDAHDPSGECDLAVVGAGIVGLAVARELHRRHPRLSVRLLERE
                     DAVARHQTGRNSGVIHAGIYYAPGSLRARLCVEGARELYEYCERAGIEHERRGKVIVA
                     LDASELPALDELERRGRANAVPGLRRLDRDGLLELEPHAAGVAALHSPATGVVDFAA"
     gene            672..4145
                     /locus_tag="VF517_14170"
     CDS             672..4145
                     /locus_tag="VF517_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8054133.1"
                     /translation="MTYATPTITIGQGDTLTFTNLDNLARHDLLGHDGKFGSDLLGAG
                     ESGPVKGVESLEQGQYQFHCSLHGWMQGVLNVGPAGSGGPSGEVTGGGGGGATPSAPT
                     QGNQDPYDAYLHATKGSVGDASWPFYGNTLAGTRDGGKDGPSAADVPNLGVAWSFYSS
                     EGDFTGTPVVSKGLVLSGSNAGKVYAIDATTGRQRWAHSTGAVINGSLAVSGNRVFVP
                     IAQPHAPKVMALDLKTGKQLWVTQISNQKNSDVYGSPVVWNGRVFMGVSALYGEYGDA
                     DVNVRGQVVALSERTGKLKWNTYTVPPGHDGGAVWTTPAVDAKTGRLYVGTGNAYHEP
                     SADTTDSMLSLDGKTGAIVGKFQATAGDVWNGTSNAAEGPDYDFGASPQLFEGPDGRK
                     LVGEGQKAGVYHALDRATMERQWSTTVGPGAFVIGGIIGSTATDGQRIYGPSTIGGEL
                     WSIDNKGGYQWFTSDGGPLKFNATSVANGVVYTTDMTGFLVAREASTGIVLNRIPLGG
                     PSWGGVSVAGGTVFAAVGTQSSAGYIAAYRVRQGDEEGAAAEHYEDNPEQDPDEYLAG
                     QERTEKCSAAKAKLKKAKGKKAKRKWKKRVRKYCTPPKEEHEHAEGEEHEGEHQHADN
                     TAQGGAVLKASRQRGDRYVPKAPGTTEHLSLYYGPYTMPPGWDMNRLDLELPTQNGFL
                     IALEPEMRRVQDLSTPMHTEAHIHHAHWFGFDPGNAEDRYFNQLGPGTHEWVFGNGDE
                     ETRADFRRRTAAEPNGPVYGNFLPAGRNQEVIYMLHNKTAQPMEVWIVLDVVFQHGTR
                     QQLEELTGRQYHDVAGMLMGRTFSVPNEPNGDGKWENIRDQKTADGKDRPIEFVAPRD
                     GTIVGTGGHLHPGGTKVTVENYGSTEDPCPADKKGRGYGGTLLLDSDIINRNAPYSED
                     YQTEVTHPAWRAPLRKGDRLRISGTYANKGRAYYYAMTHAGFYFDYDQAPKGHCKPYI
                     VGPAKKKVKDPTAGVPNRPWTMEDEYCGWGKAKPCEKPEEKPAEDAFANQNVVHVANF
                     QYLPGDRSSSTAAGSKIPSVKEGESITFVNDDQFANIRHTITTCPWPCNGRYVANYPH
                     ADGNWDSDTLGYDPIDGGTPNPMAKTPPDLKAGLYTYFCRIHPWMRGAFRIEPATPAG
                     GGAAGLTLPGG"
BASE COUNT          770 a         1594 c         1510 g          514 t
ORIGIN      
        1 ggccgcgaag tcgacgacgc cggtcgccgg cgagtgcagc gcggccacgc ccgcggcgtg
       61 cggctcgagc tcgagcagcc cgtcgcggtc gagccgtcgc agccccggca cggcgttggc
      121 acggccgcgg cgctcgagct cgtcgagcgc cggcagctcg ctcgcgtcga gcgcgacgat
      181 caccttgccg cggcgctcgt gctcgatgcc cgcccgttcg cagtactcgt acagctcgcg
      241 cgcaccctcc acgcacaggc gcgctcgcag cgaccccggc gcgtaataga tacccgcgtg
      301 gatcacgccg ctgttgcgtc cggtctggtg ccgcgcgacc gcgtcctcgc gctcgagcag
      361 ccgcacgctg aggcgagggt ggcgccggtg cagctcgcgc gccaccgcca gcccgacgat
      421 gcccgcgccg acgacggcga ggtcgcattc accgctcgga tcgtgggcgt cgcgcatcac
      481 ttcgagtttc acaaacacac ccagggggca gaaagggcgc gcaggggctg gttctggtct
      541 acacgggggt gcaattgcca ggacgtcgcg gccgaggccg cgttcttcta atagtcggag
      601 ccgtcgtcgc cggggcactg agcgccgcac cggccgcatc cgccgccgag cagcaggcct
      661 acgcggcggg catgacctac gccacgccga cgatcacgat cggccagggc gacaccctca
      721 ccttcacgaa cctcgacaac ctcgcccggc acgacctgct cggccacgac gggaagttcg
      781 gcagcgacct gctcggcgct ggcgagtccg gtccggtgaa gggggtcgag tccctcgagc
      841 agggccagta ccagttccac tgctccctcc acggttggat gcagggcgtg ctgaacgtcg
      901 gaccggccgg ctcgggcggc cccagcggtg aggtaaccgg cggtggcggc ggcggtgcga
      961 ccccgagcgc gcccacccag ggcaaccagg atccgtacga cgcgtacctg cacgcgacaa
     1021 agggctcggt cggcgacgcg agctggccgt tctacggcaa cacgctcgcc ggcacccgcg
     1081 acggcggcaa ggacggcccg agcgccgcgg acgtcccgaa cctcggcgtc gcctggagct
     1141 tctactcgag cgagggcgac ttcaccggca cgcccgtcgt gtccaagggc ctcgtgctct
     1201 ccggctcgaa cgccggcaag gtctacgcga tcgacgcgac caccggccgc cagcgctggg
     1261 cccacagcac gggcgcggtg atcaacggca gcctcgccgt cagcggcaac cgcgtgttcg
     1321 tcccgatcgc gcagccgcac gcgccgaagg tgatggcgct cgacctgaag acgggcaagc
     1381 agctctgggt cacgcagatc tcgaaccaga agaactccga cgtgtacggc agcccggtcg
     1441 tctggaacgg ccgcgtcttc atgggcgtct cggcgctcta cggcgagtac ggcgacgccg
     1501 acgtgaacgt ccgcgggcag gtcgtcgcgc tgagcgagag gaccggcaag ctgaagtgga
     1561 acacgtacac ggtgccgccg ggccacgacg gcggcgcggt gtggaccact ccggcggtcg
     1621 acgcgaagac cggccgcctc tacgtgggca ccggcaacgc ctaccacgag ccctccgccg
     1681 acacgaccga ctcgatgctc tcgctcgacg ggaagaccgg cgcgatcgtg ggcaagttcc
     1741 aggccacggc cggcgacgtg tggaacggga cgagcaacgc ggccgagggc cccgactacg
     1801 acttcggcgc ctcgccccag ctcttcgagg gccccgacgg aaggaagctc gtcggcgagg
     1861 ggcagaaggc cggcgtctac cacgcgctcg accgcgcgac catggagcgc cagtggagca
     1921 cgaccgtcgg ccccggcgcg ttcgtgatcg gcggcatcat cggctcgacc gccaccgacg
     1981 gtcagcggat ctacggcccc tccaccatcg gcggcgagct ctggtcgatc gacaacaagg
     2041 gcggctacca gtggttcacc agcgacggcg gccccctcaa gttcaacgcg acgtcggtcg
     2101 ccaacggggt tgtctacacc accgacatga ccggcttcct ggtcgcgcgc gaggcgtcga
     2161 cgggcatcgt tctgaaccgg atcccgctag gcggcccgag ctggggcggg gtatcggtcg
     2221 cgggcggcac ggtgttcgcg gcggtcggca cgcagtcgag cgcgggctac atcgctgcct
     2281 accgcgtgcg ccagggcgac gaggagggcg ccgccgccga gcactacgag gacaaccccg
     2341 agcaggaccc ggacgagtac ctcgccgggc aggagcgcac ggagaagtgc tcagccgcga
     2401 aggcgaagct caagaaggcc aagggcaaga aggccaagcg caagtggaag aagcgcgtgc
     2461 gcaagtactg cacgcccccg aaggaggagc acgagcacgc cgagggcgag gagcacgagg
     2521 gcgagcacca gcacgccgac aacaccgcgc agggtggcgc cgtgctcaag gcgagtcgcc
     2581 agcgcggtga ccgctacgtg cccaaggcgc cggggacgac cgagcacctc tcgctctact
     2641 acggccccta cacgatgccg ccgggctggg acatgaaccg gctcgacctc gagctgccca
     2701 cgcagaacgg cttcctgatc gcgctcgagc cggagatgcg ccgcgtgcag gacctcagca
     2761 ccccgatgca caccgaggcg cacatccacc acgcgcactg gttcggcttc gaccccggca
     2821 acgccgagga caggtacttc aaccagctcg ggcccggcac gcacgagtgg gtgttcggca
     2881 acggcgacga ggagacccgc gctgacttcc ggcgccgcac ggccgccgag ccgaacggcc
     2941 ccgtctacgg caacttcctg ccggccggcc gcaaccagga agtcatctac atgctgcaca
     3001 acaagaccgc gcagccgatg gaggtctgga tcgtcctcga cgtggtcttc cagcacggca
     3061 cgcgccagca gctcgaggag ctcaccggcc gccagtacca cgacgtcgcc ggcatgctga
     3121 tgggccgcac cttcagtgtg ccgaacgagc cgaacggcga cggcaaatgg gagaacatcc
     3181 gcgaccagaa gaccgccgac ggcaaggacc ggccgatcga gttcgtggcc ccgcgcgacg
     3241 gcacgatcgt cggcaccggc gggcacctgc acccgggcgg aacgaaggtg accgtcgaga
     3301 actacggcag caccgaggac ccgtgtcccg ccgacaagaa gggccgcggc tacggcggca
     3361 cgctgctgct cgactccgac atcatcaacc gcaacgcgcc gtactcggag gactaccaga
     3421 ccgaggtcac ccatcccgcg tggcgcgctc cactgcgcaa gggcgaccgg ctccgaatat
     3481 ccggcaccta cgccaacaag ggcagggcgt actactacgc catgacccac gcgggcttct
     3541 acttcgacta cgaccaggcg ccgaagggcc actgcaagcc atacatcgtc ggcccggcga
     3601 agaagaaggt gaaggacccg accgcgggcg tgcccaaccg gccgtggacg atggaggacg
     3661 agtactgcgg ctggggcaag gcgaagccgt gcgagaaacc cgaggagaag ccggccgagg
     3721 acgcgttcgc gaaccagaac gtggtccacg tggcgaactt ccagtacctg ccgggcgaca
     3781 gatcgagctc gaccgcggcg ggcagcaaga tcccgtcggt caaggagggc gagtcgatca
     3841 cgttcgtcaa cgacgaccag ttcgccaaca tccgtcacac gatcaccacg tgcccgtggc
     3901 cgtgcaacgg gcgctacgtg gccaactacc cgcacgccga cggcaactgg gactccgaca
     3961 cgctcggcta cgacccgatc gacggcggca cgccgaaccc gatggcgaag acacccccgg
     4021 acctgaaggc cggcctctac acctacttct gccgcatcca cccgtggatg cgcggcgcct
     4081 tccggatcga gcccgcgacc cccgcgggcg gcggcgcggc cgggctcacc ctgccgggcg
     4141 gctagcgccc ccgcggggcg gcttccgccg gcgcggccag gccgcacccc gccggccggc
     4201 accgccgggg ggcgggcgcc ccccggccgc ctgggccgtt gcatcgacgt tgatctgtat
     4261 agtcatctac agatgactgt cgatctaacg ctcgccccga agcagaagcg ccccgcgggc
     4321 gctacgtgct gcgcgccggt ggcgcggccc gacgtgtcgg cggccgaggc ggagcggctc
     4381 gccgctgt
//
LOCUS       DASVPL010000260        14204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_304959, whole genome shotgun sequence.
ACCESSION   DASVPL010000260 DASVPL010000000
VERSION     DASVPL010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14204
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304959"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="otsB"
                     /locus_tag="VF517_14175"
     CDS             complement(<1..779)
                     /gene="otsB"
                     /locus_tag="VF517_14175"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX8054134.1"
                     /translation="MSAPTAETLRPLTEAPDRTALLFDIDGTLAPIVERADDATVPKQ
                     AAQLLGALATRYACVACISGRAAADARRLVGVGRIAYAGSHGAEILAPGARAAVAIPA
                     FATWADPIHRFVAERDDPELRRLRVRIEDKGPIMAFHWRGAPDEGTAETRVQAIAAEA
                     EAGGLAIHWGRKVLEVRPPVPISKGRAVREFVRSSGARAAMYAGDDATDLDAFDALDE
                     LAAEGELDLALRVGVRSDEGPAAIVERADLVVDDMIDVLERL"
     gene            800..1159
                     /locus_tag="VF517_14180"
     CDS             800..1159
                     /locus_tag="VF517_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8054135.1"
                     /translation="MSPRLVRLALIGSAAGAFSGLFGVGGGTVIVPLLVLWFGFEERE
                     ATGTSLMAIVAIALIAAGVQGLHGNVDPLKALLVGIPALGGVLAGTALQQRVSERMVA
                     GAFAALLVVSAILLVIG"
     gene            1159..1542
                     /locus_tag="VF517_14185"
     CDS             1159..1542
                     /locus_tag="VF517_14185"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8054136.1"
                     /translation="MDLATVAGVIGLGFAAGVTSGMLGVGGGVIFVPALVFFLDQGQL
                     DAESTSLLAIVPVAILGAWRQHGYGNLRLREGLIVGMLAPPGILLGTTIANEVSERTL
                     ELSFAALQLFFAISMARRALAPRDG"
     gene            1570..1977
                     /locus_tag="VF517_14190"
     CDS             1570..1977
                     /locus_tag="VF517_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054137.1"
                     /translation="MAKRILALVSEAIDAEALRSALGGEDLDGAEVLVIAPALNSRRR
                     FIMADVDGAIERAEAVQQETVERLDEGGVDAAGGTGESDPMLALQDALVTFDADEIVL
                     FTHSGGKSNFLEEGLVDEASARFEPPVRHMVVE"
     gene            1980..2912
                     /locus_tag="VF517_14195"
     CDS             1980..2912
                     /locus_tag="VF517_14195"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX8054138.1"
                     /translation="MDLQYDAWLRERSFHHSRFPAERLAAERTATVSVCVPAREEART
                     IGPIVRDLVGLREAGVIDQVVVADAESADGTGRIAAEEGAEVHVQSELLSGYGPCAGK
                     GDAMWRALSVLHGEVVLFVDADSEDFGPHFACGLLGPLLLDETIHYVKAFYRRPFKLD
                     SACAPEGGGRVTELTARPLLNLFYPQLALFAQPLAGEIAARRSLFERLPWSSGYAIEI
                     TQLIDAWAEVGLWGMAQVDLDVRQNRHQSLHDLGPMAYAVLRGVTERLVREGRLTAEQ
                     RDAFVTPGPHGLEEHRVELVERPPMATLAAGASA"
     gene            complement(2933..3088)
                     /locus_tag="VF517_14200"
     CDS             complement(2933..3088)
                     /locus_tag="VF517_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054139.1"
                     /translation="MTNQETENQQQGSSTSPPEETKDATTPPANPEVDQEAVEKGQEN
                     IDRISGN"
     gene            3181..4101
                     /locus_tag="VF517_14205"
     CDS             3181..4101
                     /locus_tag="VF517_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054140.1"
                     /translation="MRRRRAVAALAGAVALIVVVALLVGGDDGESDRAGEDRAAAAAP
                     PELPRGGRRILPRFRLVAFYGAPQAGELGALGIGTPDQAGRRLARQARTYRRPGRPVL
                     PVFELIAVVANGSPGRDGRYRTRQGAATIRRYLAAARRARALLLLDIQPGRADFLAEM
                     RALEPFLREPDVGLALDPEWHVGAGQVPGQVLGSVDAGVVNTVSARLAAIVRERRLPD
                     KLLVVHQFTANMVRRKPTLARPPGVALVLDVDGFGTQAQKIAKYRELTRGGGPRFAHG
                     LKLFFREDTDLMSPPEVLRLRPQPQLVVYE"
     gene            complement(4109..5062)
                     /locus_tag="VF517_14210"
     CDS             complement(4109..5062)
                     /locus_tag="VF517_14210"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054141.1"
                     /translation="MPTVETVNGPVEADQLGRTLIHEHFRTTDEATRFQFPHLYDEDA
                     EWDAALADANAVKGHGIQTVVEPSAMFLQRDARFSKRVADESGLQIVLATGVYTYDHL
                     PQPLKNRDEDALAEIFVHDIEQGMQGTGIKAAFLKCAADAEGVGENVAKVHRAVARAS
                     QRTGRPIMAHSHPASGTGLEQMRIFEEEGVDPAKVQIAHTGDTDDLDYIEKLLERGPW
                     IGLDRYGLDIFLPTDKRNATTLALLERGYAERMFLSQDFCSTIDWFPPEVQQQLKENV
                     VPNWSMTFLFEEVIPYLKENGMTDEQLDQMMVENPKRWLTA"
     gene            5156..6067
                     /locus_tag="VF517_14215"
     CDS             5156..6067
                     /locus_tag="VF517_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054142.1"
                     /translation="MTRLRTLTLVLLAGLLLLAPAAPAKTAWKRLGEARTPQLETDPR
                     YAAYEDPNGVTRLFDSETNRRTKHRRPIADCPFTNLGGGRIMWACRAYPVHIAFETIR
                     TGRIGQRDLEAGDSAGGAHYIGKYWVKGAVNDANGNQYEAWWRLSDGTRGPEVTGARR
                     IDDLDRPGLVRELCSPLQRKPNPFFGMTGDTRPYAAYQFDGRFGLTADGFQWVLQRCG
                     QKPKPVTLGLNGGELTAGRIAYFDRGDIVVLDAATRRKTTWSVTDIEPTADGAVVRVT
                     ANRIFVAVFRGADGFAPSRVYTARLPK"
     gene            complement(6125..7060)
                     /locus_tag="VF517_14220"
     CDS             complement(6125..7060)
                     /locus_tag="VF517_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX8054143.1"
                     /translation="MSDTVRVAAIQATPVILDVDATTDKAAALLAEAAGEGARLAVLP
                     ETFLSVYPSNAWAAGATAFTGWDDLWERMWESAVDVCGPHVARLVEVCREHELHCVIG
                     VNEREDERPGTLYNAMLTLGPEGLLHRHRKLMPTMQERVFHGVGAGDDLDVTELPGIG
                     RVGGLICWENRMPLARYAVYKHGPQIWAAPTADDTGGWLASMRHIAIESGAFVVSVPQ
                     YIPASAFPEDFPVPLPDGMDVFGKGGAAVVEPTWGKVIAGPLRDSEGILYADCDLRET
                     LRAKRWFDVVGHYGREDVLAQPAGIPVRADEPPPG"
     gene            complement(7064..7711)
                     /locus_tag="VF517_14225"
     CDS             complement(7064..7711)
                     /locus_tag="VF517_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054144.1"
                     /translation="MQGGGILDESTLVVGEQANWLVRKLAPAETRVCPVWDRDGQQIG
                     QVEPTDRDSAGGKAVRFGLALLLTSSTRRNDRRLRLVDASGSTLLDLHIAGGALYVNG
                     AGGEEIGMVRNVSDRQWLRAEFTTEVPKKKLFFRWPESVATARTKPDNPPYEFEITNG
                     SGGTIARLTNDGDHRNVLAIHERPDDRVRALLVGFACGLVDRVWLTKPQDSGGGI"
     gene            7836..8483
                     /locus_tag="VF517_14230"
     CDS             7836..8483
                     /locus_tag="VF517_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054145.1"
                     /translation="MQEPPSTPADAAQTEDARSKRIEVWIAGLLGVVAVLTAFATYKA
                     VLEDGDTLANFNEGIALNVQAAERDTAGLQEYSTDQATYLEYVKATQGDDTDLSSYFR
                     ETLFSPELEEGVKWVDEGPKDAISPFQEDSPYARPEWSEADKLRTKTDAKFKEAGIHD
                     EDGDKYVLATVVLSIALFLLGIAGVNRQFRVQRGLTALGAVVSLIGLIQILAVAL"
     gene            8518..9453
                     /locus_tag="VF517_14235"
     CDS             8518..9453
                     /locus_tag="VF517_14235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8054146.1"
                     /translation="MAQPRFDEWQLDLHGHRAIYRVAGSGPPVVLIHGMVNSSRHWEN
                     VALRLADDYTVVAPDLIGHGDSATPRGDYSLGAHAASIRDLLTAIGIDRATIVGHSLG
                     GGVAMQFFYQFPQRTERLVLVSSGGLGGEVSPLLRSAAVPGASALLSLAAHPRLVDAL
                     AGAGARLRERGSGAGVPLQAVARALRPLERPGAREAFLQTLRAVIDVKGQRVSARDRL
                     YLLESMPTLVVWGERDNTIPLAHGLDTHRAVPHSRFATLPRAAHFPHLEDPEGLAAVL
                     REFLETTEPAPVEDADWGGLIARRAPRSRREPAQV"
     gene            complement(9468..11711)
                     /locus_tag="VF517_14240"
     CDS             complement(9468..11711)
                     /locus_tag="VF517_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX8054147.1"
                     /translation="MAERTAYATCPLCEATCGLEVSVDGGAVTGIRGDRDDVFSQGFI
                     CPKGGSLRALHDDPDRLRTPLVRRDGELVEASWDEAFAEIDRRLPPLLESHGRDAVAV
                     YVGNPAAHSLSFQTFGRVLLKALGTGNIYTASTVDQMPKQISSALMFGGGLSVPVPDV
                     DRTDHLLILGANPLASNGSLLTAPDMRGRLRRIRERGGKVVVIDPRRSRTAEHADEHH
                     FIRPGTDALLLFALVHAIFDEGLEDLGTVGDHVEGLEVVRELARGFAPEDAAGACGID
                     AGTIRRMARELATAERAAVYGRIGTCTQEFGTLASWLVDVLNAITGNLDRPGGAMFPL
                     AAAGQSNSQGARGRGRGLTRGRWHTRVRGLPEAFGELPVACLAEEIETPGEGQVRALI
                     TVAGNPAVSTPNAQRLAGALSELDFMVSIDVYVNETTRHADVVLPGPSPLERSHYDLA
                     LYQLAARNVANYSAPVLEASMPQEWETALRLTGVVTGQGPAADIDAIDDFVAAEAVRR
                     EVARAGGALEGRDAAEILAALEHRRGPERLLDLLLRSGPYGDAFGARPDGLSLAALEA
                     APHGVDLGPLEPRVPDVLRTPSGRIELAPEPLVADVERLRAALGRHVNGGMVLIGRRQ
                     LRSNNSWMHNIAPLVRGKERCTAHVHPDDAARLGLADGEPVQVSSRAGSIEVQAEVTD
                     AVMPGVVSIPHGWGHDVDGVRMEVATAHAGSNSNVLADETLVDPLSGNAVLNGIPVEL
                     APVAVPV"
     gene            complement(11749..12474)
                     /locus_tag="VF517_14245"
     CDS             complement(11749..12474)
                     /locus_tag="VF517_14245"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8054148.1"
                     /translation="MRATTVRFSDDLWSLLEQEASAAGVSAAQFVRDATVLRIAYLMG
                     RRGEPGIEETLQRLSGLPERDNGPVPPANVMAAVRDVERLRTLRDTGLLDSPRQENFD
                     RHARIASEALDAPVALVSLIDEDRQFFQSCIGLPEPWQSQRETPLSHSFCQHAVASRE
                     PLIVDDAREHPVLKDNLAIRDLRVVAYAGIPLISGDGQALGTLCTIDDHPRHWTKHQV
                     GLLKDIAESVTREIEIARATNSG"
     gene            12584..13111
                     /locus_tag="VF517_14250"
     CDS             12584..13111
                     /locus_tag="VF517_14250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8054149.1"
                     /translation="MAAGAVREQAGAAGLAGTYERHAENVRRAAYSVTHDRALAEDVT
                     QDVFLALWARPERYDPARGSVENLLRVMARSRALDAARRNSAARRAQDRLDAEPVAAS
                     EPDPAEAAATSLRAGRLRRAVQGLPPEQRDAIALAYWGGLTAEEVAHRRGIPHGTAKS
                     RIRIGLKKLRSDFDG"
     gene            13170..>14204
                     /locus_tag="VF517_14255"
     CDS             13170..>14204
                     /locus_tag="VF517_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8054150.1"
                     /translation="MAKAPPGAPPGPPLPRVVQTLGFLTAGTRYLEACRRRYGDVVWF
                     RTLFDPGFAMVFDPDVVRDVFQAPPDRLRAGEANALLSTVLGPRSVLVLDGAEHLRHR
                     KLMLPPFHGRRLTAHEEVMRESADRSIDAWPVGGEFALLPHMYVLTLDVIATAVFGIE
                     EDDRREELKRRIRTFLETAGSPLRVILLMLSRGRIRDEAAQRQFDARREAMDELILDE
                     IAQRRGAPDIEERDDALSLLLLARDEDGAGLTDAEVRDQLVTLLVAGHETTAAGIAWT
                     LDLVYRDPAVLERARTAAREGETVYLDAVVKESLRIRPVVPGIGRVVREEPFVAGGYS
                     IPPGVEINPSI"
BASE COUNT         1834 a         5128 c         5200 g         2042 t
ORIGIN      
        1 aggcgctcga ggacgtcgat catgtcgtcc acgacgaggt cggcgcgctc cacgatcgcg
       61 gccggcccct cgtcggaccg gacgccgacc cgaagtgcga ggtcgagctc gccctcggcc
      121 gcgagctcgt cgagggcgtc gaaggcatcg aggtcggtcg cgtcgtcgcc ggcgtacatc
      181 gccgcccgcg cgccggacga gcgaacgaac tcgcgcacgg cgcggccctt gctgatcggc
      241 accggcgggc gcacctcgag caccttgcgt ccccagtgga tcgcgaggcc gccggcctcg
      301 gcctcggcgg cgatcgcctg gacccgcgtc tcggcagtgc cctcgtcggg cgcgccgcgc
      361 cagtggaagg ccatgatcgg ccccttgtcc tcgatccgca cgcgcagccg gcgcagctcg
      421 ggatcgtcgc gctcggccac gaagcggtgg atcgggtcgg cccaggtggc gaacgcgggg
      481 atcgccacgg ccgcacgcgc gccgggggcc aggatctccg ccccgtgcga ccccgcgtag
      541 gcgatccggc caacgccgac caggcgccgc gcgtccgccg cagcgcggcc ggagatgcat
      601 gcgacgcagg cataacgggt ggcgagagcg ccgaggagct gcgcggcctg cttgggcacc
      661 gtcgcgtcgt ccgcgcgctc gacgatcggg gcgagcgtgc cgtcgatgtc gaacagcagg
      721 gccgtacggt cgggagcttc agtcagcggc cgcagcgttt cggccgtggg ggctgacacg
      781 cgtcctagta tgcccggcca tgtcaccgcg gctcgtccgg ctggcgctga tcggcagcgc
      841 cgccggagcc ttcagcgggc tgttcggggt tggcggcggc accgtgatcg tcccgctgct
      901 cgtgctgtgg ttcggcttcg aggagcgcga ggccaccggc acgtcgctca tggcgatcgt
      961 ggcgattgcg ctgatcgcgg cgggggtgca ggggctccac ggcaacgtcg acccgctgaa
     1021 ggcgctgctc gtcggcatcc ccgcgctggg cggcgtgctg gccggcaccg ccctccagca
     1081 gcgggtctcg gagcggatgg tggccggcgc gttcgcggcg ctgctggtcg tgtcggcgat
     1141 cctcctggtg atcggctagt ggacctcgcc accgtcgccg gcgtgatcgg gctcggcttc
     1201 gccgccggag tgacgagcgg catgctcggg gtgggcgggg gcgtgatctt cgtgccggct
     1261 ctcgtgttct tcctcgacca ggggcagctc gacgccgagt cgacgtcgct gctcgcgatc
     1321 gttcccgttg cgatcctggg ggcgtggcgc cagcacggat acgggaacct gcgcctgcgg
     1381 gagggcttga tcgtcgggat gctggccccg cccgggatcc tgctgggcac cacgatcgcc
     1441 aacgaggtca gcgagcggac gctcgagctg tcgttcgcgg cgctccagct cttcttcgcg
     1501 atcagcatgg ccaggcgcgc gctcgcgccg cgtgatggtt gagcgcccgt tcgggtatac
     1561 acctagccca tggcgaagag gatcctggca ctggtatcgg aggcgatcga cgcggaggcg
     1621 ctgcgcagcg cgctcggcgg cgaggacctc gacggtgcgg aggtgctcgt gatcgcgccc
     1681 gcgctgaaca gcaggcggcg cttcatcatg gccgacgtcg acggcgccat cgagcgcgcg
     1741 gaggcggtgc agcaggagac cgtggagcgg ctcgacgagg gcggggtgga cgccgccggc
     1801 ggcaccggcg agtccgatcc gatgctcgcg ctgcaggacg cgctcgtcac gttcgacgcg
     1861 gatgagatcg tgctcttcac gcactcgggt gggaagagca acttcctcga ggagggattg
     1921 gtagacgagg catcggcgcg gttcgagccg cccgtccgtc acatggtcgt ggagtagccg
     1981 tggatctgca gtacgacgcc tggctgcgcg agcgcagctt ccatcacagc cggttccccg
     2041 ctgagcggct ggccgccgag cggaccgcga ccgtgtcggt ctgcgtgccg gcgcgcgagg
     2101 aggcgcgcac gatcgggccg atcgtgcgcg acctggtcgg gctgcgcgag gccggggtga
     2161 tcgaccaggt ggttgtcgcg gacgccgagt ccgccgacgg caccgggcgg atcgccgccg
     2221 aggagggcgc cgaggtgcac gtgcagtcgg agctcctgtc cggctacggg ccgtgcgccg
     2281 ggaagggcga cgcgatgtgg cgcgcgctgt cggtcctgca cggcgaggtg gtgctgttcg
     2341 tcgacgccga ctcggaggac ttcggccccc acttcgcgtg cggcctgctg gggccgctgc
     2401 tgctcgacga gaccatccac tacgtcaagg cgttctaccg acgcccgttc aagctcgaca
     2461 gcgcgtgcgc gccggagggc ggcgggcgcg tgaccgagct gacggcgcgg ccgctcctca
     2521 acctcttcta tccacagctc gccctgttcg cgcagccgct ggccggcgag atcgccgcgc
     2581 gccggtcgct gttcgagcgg ctcccgtggt cctccggcta cgcgatcgag atcacgcagc
     2641 tcatcgacgc ctgggccgag gtcgggctat gggggatggc gcaggtcgac ctcgacgtgc
     2701 gccagaaccg ccaccagtcg ctgcacgacc tggggccgat ggcgtacgcg gtgctccgcg
     2761 gcgtgaccga gcgcctcgtg cgcgagggcc ggctcaccgc ggagcagcgc gacgcgttcg
     2821 tgacccccgg cccgcacggg ctcgaggagc accgggtcga gctggtcgaa cggccgccga
     2881 tggcgacgct ggcggccggc gcgtccgcct agccggccgc catccgggcg ggctagttcc
     2941 cggagatccg gtcgatgttc tcctgcccct tctcgacggc ctcctgatcg acctcggggt
     3001 tcgccggcgg ggtcgtggcg tccttcgtct cctccggggg gctggtggag gagccctgct
     3061 gctggttctc ggtctcttgg ttggtcatgt cggtagcggt acccggaggg ccgcgagata
     3121 agcccgctcg accgcgacgc gctggcctgg cggcgcgcgg ccgctcggcg gcgggcgcag
     3181 gtgcgccggc ggcgggccgt cgcggcgctg gccggtgccg tggcgctgat cgtggtcgtg
     3241 gcgttgctgg tcggagggga cgacggcgag tccgatcgcg ccggcgagga ccgggcggcc
     3301 gccgccgcgc cgccggagct cccgcgcggg ggacggcgga ttctgccgcg ctttcggctg
     3361 gtggcgttct acggcgctcc gcaggccggc gagctcgggg ccctcggcat cggcacgccg
     3421 gaccaggcgg gtcgcaggct ggcgcgccag gcgcgcacgt acaggcggcc cggccgaccc
     3481 gtgctgccgg tgttcgagct gatcgcggtg gtggccaacg gctcgcccgg ccgcgacggg
     3541 cgctaccgca cacgccaggg cgcggcgacg atccggcgct acctggcggc ggcgcggcgg
     3601 gcgcgggcgc tgctgctgct cgacatccag cccggccggg cggacttcct cgccgagatg
     3661 cgcgcgctcg agccgttcct gcgcgagccg gacgtgggcc tggcgctcga ccccgagtgg
     3721 cacgtcgggg ccggacaggt gccgggccag gtgctcggct cggtggacgc gggggtcgtg
     3781 aacaccgtgt ccgcgcgact ggcggcgatc gtgcgcgagc ggcggctgcc ggacaagctg
     3841 ctcgtggtgc accagttcac ggcgaacatg gtgcggcgca agcccacgct cgcgcgcccg
     3901 cccggcgtgg cgctcgtgct cgacgtcgac ggcttcggca cgcaggcgca gaagatcgcg
     3961 aagtaccgcg agctgacgcg cggaggcggc ccgcggttcg cacacggact gaagctcttc
     4021 ttccgcgagg acaccgacct gatgtcgccg cccgaggtgc tgcgcctgcg gccgcagccg
     4081 cagctcgtgg tctacgagta ggcgccgctc aggcggtgag ccagcgcttc gggttctcca
     4141 ccatcatctg atcgagctgc tcgtcggtca tcccgttctc cttcaggtac gggatcacct
     4201 cctcgaacag gaacgtcatg ctccagttcg ggacgacgtt ctccttgagc tgctgctgca
     4261 cctccggcgg gaaccagtcg atcgtcgagc agaagtcctg cgagaggaac atgcgctcgg
     4321 cgtagccgcg ctcgagcagg gcgagggtgg tcgcgttgcg cttgtcggtg ggcaggaaga
     4381 tgtcgagccc gtagcgatcg aggccgatcc acgggccgcg ctcgaggagc ttctcgatgt
     4441 agtcgaggtc gtcggtgtcg ccggtgtggg cgatctggac cttggcgggg tcgacgccct
     4501 cctcctcgaa gatgcgcatc tgctcgaggc ccgtgccgct cgcgggatgc gagtgggcca
     4561 tgatcggccg gccggtccgc tggctcgcgc gcgcgaccgc gcggtgcacc ttcgccacgt
     4621 tctcgccgac gccctcggca tcggcggcgc acttgaggaa ggcggccttg attccggtgc
     4681 cctgcatgcc ctgctcgatg tcgtgtacga agatctccgc gagggcgtcc tcgtcgcggt
     4741 tcttcagcgg ctgcggcagg tggtcgtagg tgtagacgcc ggtggcgagc acgatctgga
     4801 ggccagactc gtcggccacg cgcttggaga agcgtgcgtc gcgctggagg aacatcgcgc
     4861 tcggctcgac cacggtctgg atgccgtggc ccttgacggc gttggcgtcg gcaagcgccg
     4921 cgtcccactc cgcgtcctcg tcgtagaggt gcgggaactg gaagcgggtc gcctcgtcgg
     4981 tggtgcggaa gtgctcgtgg atgagcgtcc ggccgagctg gtcggcctcg acgggcccgt
     5041 tcacggtctc gactgtgggc atcttcggtc tctcctcccg ctggttgcgg cgcgtacgct
     5101 acggggaatg gcgccggcac cgcagcccgg ccgcggttgg cgtgtttgca ccccagtgac
     5161 caggctccgc acgctcacgc tcgtgctgct cgccggcctt ctcctgctgg ctccggcggc
     5221 gccggcgaag acggcttgga agcgcctcgg cgaggcgcgg acgccccagc tcgagaccga
     5281 cccgcgctac gcggcgtacg aggaccccaa cggcgtgacg cggctcttcg acagcgagac
     5341 gaaccgccgg accaagcacc ggcggccaat cgccgactgc ccgttcacga acctcggtgg
     5401 cggccggatc atgtgggcgt gccgggccta cccggtgcac atcgcgttcg agacgatccg
     5461 cacgggcagg atcgggcaac gcgacctcga ggccggcgac agcgccggcg gcgcgcacta
     5521 catcggcaag tactgggtca agggcgcagt gaacgacgcg aacggcaacc agtacgaggc
     5581 ctggtggcgg ctgtcggacg ggacgcgcgg tccggaggtc accggcgcgc ggcggatcga
     5641 cgacctcgac cgcccggggc tcgtgcgcga gctgtgctcg ccgctccagc gcaagcccaa
     5701 tccgttcttc ggcatgaccg gggacaccag gccctacgcg gcctaccagt tcgacggccg
     5761 cttcggcctc accgccgacg gcttccagtg ggtcctccag cgctgcgggc agaagcccaa
     5821 gccggtgacg ctggggctca acggcggcga gctgaccgcg ggccggatcg cctacttcga
     5881 ccgcggcgac atcgtcgtgc tcgacgcggc cacgcgccgg aagacgacgt ggtccgtcac
     5941 cgacatcgag cccacggcgg acggcgcggt cgtgcgcgtc acggccaacc gcatcttcgt
     6001 cgcggtgttc cgcggcgcgg acggcttcgc gccctcgcgg gtgtacacgg cgcgcctgcc
     6061 gaagtagggc tcgcggcgcg tccgccgcgc gcctgccggc acgattcgct ccgagggtgg
     6121 gcggctagcc gggcggcggc tcgtcggcgc gcacggggat ccccgccggc tgcgcgagca
     6181 cgtcctcgcg cccgtagtgt ccgaccacgt cgaaccagcg cttcgcgcgc agcgtctcgc
     6241 gcaggtcgca gtccgcgtac aggattccct cgctgtcgcg gagcggaccc gcgatgacct
     6301 tgccccaggt cggctcgacg accgccgcgc cgcccttgcc gaacacgtcc atcccgtcgg
     6361 gcagcgggac cgggaagtcc tccggaaacg cgctcgccgg gatgtactgc ggcaccgaga
     6421 ccacgaacgc gcccgactcg atcgcgatgt gcctcatgct cgcgagccag ccgcccgtgt
     6481 cgtcggcggt cggggcggcc cagatctgcg ggccgtgctt gtacacggcg tagcgggcca
     6541 gcggcatccg gttctcccag cagatcagcc cgcccacgcg cccgatgccc ggcagctctg
     6601 tcacatccag gtcgtctccc gccccgacgc cgtggaacac gcgctcctgc atcgtcggca
     6661 tgagcttgcg gtggcgatgc agcaggccct cggggccgag cgtgagcatc gcgttgtaga
     6721 gcgtgccggg ccgctcgtcc tcgcgctcgt tcacgccgat cacgcaatgc agctcgtgct
     6781 cgcggcagac ctcgacgagg cgggcgacgt gcgggccgca cacgtccacg gcgctctccc
     6841 acatccgctc ccagaggtcg tcccagccgg tgaacgccgt ggcgccggcg gcccacgcgt
     6901 tcgaggggta gaccgagagg aacgtctcgg gaagcacggc cagtcgcgcc ccctcgcccg
     6961 cggcctcggc gagcagcgcg gcggccttgt cggtcgtcgc gtcgacgtcg aggatgaccg
     7021 gcgtcgcctg gatcgcggcc actcggacgg tgtcggacac ggcctagatc cctccgcccg
     7081 agtcctgcgg cttcgtgagc cacacgcggt ccacgaggcc gcaggcgaac ccgaccagca
     7141 gcgcacgcac gcgatcgtcc ggtcgttcgt ggatcgcgag cacgttgcgg tggtcgccat
     7201 cgttcgtgag gcgcgcgatc gtcccgccgc tgccgttcgt gatctcgaac tcgtacggcg
     7261 ggttgtccgg cttggtgcgc gccgtggcaa ccgattccgg ccagcggaag aagagcttct
     7321 tcttgggcac ctcggtcgtg aactccgccc tgagccactg tcggtcgctc acgttgcgca
     7381 ccatcccgat ctcctcaccg ccggcaccgt tcacgtagag cgccccaccg gcgatgtgca
     7441 ggtccaggag cgtgctgccg gacgcgtcca caagccgcaa ccggcgatcg tttcgccgcg
     7501 tgctggacgt gagcagaagc gccagcccga agcggaccgc cttgccgccc gccgagtcgc
     7561 gatcggtcgg ctcgacctgc ccgatctgct gcccgtcacg gtcccagacc gggcacaccc
     7621 gagtctccgc gggcgcgagc ttgcgcacca accagttggc ctgctcgccg acgacgagcg
     7681 tcgactcgtc aaggatgccc ccgccttgca tcgacgcgga ccttacaggc gaccttctcg
     7741 ggtgagcatc gtgtcaccat gggtggtcgc ggttcaccgc gaccacattc gtgccaggag
     7801 cagggatcgc cgcgtgcccg gcgtatcctc cgctcatgca agagcccccc agcacgcccg
     7861 cggacgccgc ccagaccgag gacgcgcgca gcaagcgcat cgaggtgtgg atcgccgggc
     7921 tgctcggcgt cgtcgcggtc ctcaccgcat tcgccaccta caaggccgtc ctcgaggacg
     7981 gcgacaccct ggccaacttc aacgagggga tcgccctcaa cgtccaggcg gccgagcgcg
     8041 acaccgctgg cctccaggag tactcgaccg accaggccac ctacctcgag tacgtcaagg
     8101 ccacgcaggg ggacgacacc gacctctcgt cgtacttccg cgagacgctc ttctcgcccg
     8161 agctcgagga gggcgtgaag tgggtcgacg aggggccgaa ggacgcgatc tcgccgttcc
     8221 aggaggactc gccgtacgcc cggcccgagt ggagcgaggc ggacaagctg cgcacgaaga
     8281 ccgacgcgaa gttcaaggag gccgggatcc acgacgagga cggcgacaag tacgtgctcg
     8341 ccaccgtcgt gctctcgatc gccctgttcc tgctcggcat cgccggcgtg aaccgccagt
     8401 tccgcgtgca gcgcgggcta acggccctcg gcgcggtcgt ctcgctgatc gggctcatcc
     8461 agatcctggc cgtcgcgctc tgagcgccgg cgcccgctgg cgctaacgtg ccgccgcgtg
     8521 gcgcaaccac gcttcgacga gtggcagctc gacctgcacg ggcaccgcgc gatctaccgg
     8581 gtcgccggca gcggtcctcc ggtcgtgctc atccacggga tggtcaactc ctcgcgccac
     8641 tgggagaacg tggcgctgcg gctggcggat gactacaccg tcgtcgcgcc ggacctgatc
     8701 ggccacggcg actcggccac cccgcgcggc gactactcgc tcggtgctca cgcagcgagc
     8761 atccgcgacc tgctcaccgc catcgggatc gaccgcgcga cgatcgtcgg ccactcgctc
     8821 ggcggcggcg tggcgatgca gttcttctac cagttccccc agcgcaccga gcggctcgtg
     8881 ctcgtgtcga gcggcgggct cggcggcgag gtgagcccgc tgctgcgcag cgccgccgtc
     8941 cctggtgcgt ccgcgctgct ctcgctcgcc gcgcatccgc gcctggtcga tgcgctggcc
     9001 ggcgccgggg cgcgcctgcg cgagcgcggc tcgggcgccg gcgtgccgct ccaggcggtc
     9061 gcgcgtgcac tgcgcccgct ggagcgcccg ggcgcgcgcg aggcgttcct ccagacgctg
     9121 cgagcggtga tcgacgtgaa gggccagcgc gtgagcgcgc gcgaccggct ctacctgctc
     9181 gagtcgatgc cgacgctggt ggtctggggc gagcgcgaca acacgatccc gctcgcgcac
     9241 ggcctcgaca cccaccgggc cgtgccgcac agccgcttcg ccacgctccc gcgcgccgcg
     9301 cacttcccgc acctcgagga ccccgagggg ctcgcggcgg tgctgcgcga gttcctcgag
     9361 acgaccgagc cggcgcccgt cgaggacgcc gattggggcg ggctgatcgc gcggcgggcg
     9421 ccgcgcagcc ggcgcgagcc cgcgcaggtc tagcggcgcg ggccgctcta gaccggcacg
     9481 gcgaccggcg cgagctcgac cgggatcccg ttgagcaccg cgttgcccga cagcgggtcg
     9541 accagcgtct cgtccgcgag cacgttcgag ttcgagccgg cgtgcgccgt cgcgacctcc
     9601 attcgcacgc cgtccacgtc atgcccccag ccgtggggga tgctcacgac gccgggcatc
     9661 accgcgtcgg tcacctcggc ctgcacctcg atcgatcccg cccgcgagct cacctgcacc
     9721 ggctcgccgt cggcgaggcc gagccgcgca gcgtcgtcgg ggtgcacgtg cgcggtgcag
     9781 cgctccttgc cgcgcacgag cggcgcgatg ttgtgcatcc acgagttgtt cgagcgaagc
     9841 tgccggcggc cgatcagcac catgccgccg ttcacgtggc ggccgagcgc cgcgcgcagc
     9901 cgctcgacgt cagccacgag cggttcgggc gccagctcga tgcggccgct cggcgtccgc
     9961 agcacgtccg gcacgcgcgg ctcgagcgga ccgaggtcga cgccgtgtgg cgccgcctcc
    10021 agtgccgcga gcgagaggcc gtccggccgc gcgccgaacg cgtcgccgta gggacctgag
    10081 cgcagcagca ggtcgagcag ccgctcgggc ccgcggcggt gctccagggc ggcgaggatc
    10141 tcggcggcgt cgcgcccctc cagcgcgcca cccgcgcgcg cgacctcgcg gcgcaccgcc
    10201 tccgccgcca cgaagtcgtc gatcgcgtcg atgtcggccg cggggccctg gccggtcacg
    10261 accccggtga gccgcagcgc ggtctcccac tcctgcggca tcgacgcctc gagcacgggc
    10321 gccgagtagt tggccacgtt gcgcgcggcg agctggtaga gcgcgaggtc gtagtgcgag
    10381 cgctcgagcg gcgacggtcc gggcaggacc acgtcggcgt ggcgcgtcgt ctcgttcacg
    10441 tagacgtcga tcgagaccat gaagtcgagc tcgctaagcg ctcccgcgag ccgctgcgcg
    10501 ttcggcgtgc tcaccgccgg gttccccgcc accgtgatca gcgcgcgcac ctggccctcg
    10561 ccgggcgtct cgatctcctc ggccaggcac gccaccggca gctccccgaa cgcctccggc
    10621 aacccgcgca cgcgcgtgtg ccagcgcccg cgcgtcagcc cacggccgcg accgcgcgcg
    10681 ccctgcgagt tgctctggcc agcggccgcg agcggaaaca tcgccccgcc gggccgatcg
    10741 aggttgcctg tgatcgcgtt cagcacgtcg accagccagc tcgcgagcgt gccgaactcc
    10801 tgggtgcacg tgccgatccg gccgtacacg gcggcgcgct cggcggtcgc gagctcgcgg
    10861 gccatccggc ggatcgtgcc ggcgtcgatg ccgcaggcgc ccgccgcgtc ctccggggcg
    10921 aacccgcgcg ccagctcgcg cacgacctcg aggccctcga cgtgatcgcc caccgtgccc
    10981 aggtcctcga gcccctcatc gaagatcgcg tgcacgagcg cgaacagcag cagcgcgtcg
    11041 gtgcccggcc ggatgaagtg gtgctcgtcg gcgtgctcgg cggtgcggct gcggcgcgga
    11101 tcgatcacga ccaccttgcc gccgcgctcg cggatccggc gcaggcgccc gcgcatgtcg
    11161 ggcgccgtga gcaggctccc attcgacgcc agcgggttgg cacccaggat cagcagatgg
    11221 tcggtgcggt cgacgtcggg caccggcacc gacaggccgc cgccgaacat cagcgccgag
    11281 gagatctgct tcggcatctg gtcgaccgtg ctggcggtgt agatgttgcc ggtgccgagc
    11341 gccttcagca gcacccgccc gaaggtctgg aacgacaggc tgtgcgcggc cgggttgccg
    11401 acgtacaccg ccaccgcgtc gcgcccgtgg ctctccagga gcggcggcag gcggcgatcg
    11461 atctcggcga acgcctcgtc ccagctcgcc tcgaccagct ccccgtcgcg gcgcaccagc
    11521 ggcgtgcgca gccggtcggg gtcgtcgtgc agcgcccgga ggctgccgcc cttcgggcag
    11581 atgaacccct gcgagaacac gtcatcgcgg tcgccgcgga tcccggtcac cgcgccgcca
    11641 tcgacgctca cctcgaggcc gcacgtcgcc tcgcagagcg ggcacgtcgc gtatgcggtg
    11701 cgctcggcca tcgcggaccg agagcgtacg cccttgggcg cctccgcgct agccggagtt
    11761 cgtggcgcgc gcgatctcga tctcgcgcgt gaccgactcg gcgatgtcct tcagcagccc
    11821 gacctggtgc ttggtccagt gccgcgggtg gtcgtcgatc gtgcagagcg tcccgagcgc
    11881 ctggccgtcg ccgctgatca gcgggatgcc ggcgtaggcg accacgcgga gatcgcggat
    11941 cgcgaggttg tccttgagaa ccgggtgctc gcgcgcgtcg tccacgatca gcggttcgcg
    12001 cgacgcgacc gcgtgctggc agaacgagtg gctgaggggc gtctcgcgct ggctctgcca
    12061 cggctccggg aggccgatgc agctctggaa gaactggcgg tcctcgtcga tcagcgagac
    12121 gagcgccacc ggggcgtcga gcgcctccga ggcgatccgc gcgtggcggt cgaagttctc
    12181 ctggcgcggg gagtccagca ggccggtgtc gcgcagggtg cgcaggcgct cgacgtcgcg
    12241 caccgcggcc atgacgttcg ccgggggcac cgggccgttg tcccgttccg gcaggcccga
    12301 gagccgttgg agcgtctcct cgatgccggg ctcgccgcgc cgccccatga ggtaggcgat
    12361 gcgcaggacg gtcgcgtcgc gcacgaattg ggcggcactg acgcccgccg ccgatgcctc
    12421 ctgctcgagg agcgaccaga ggtcatcaga gaaccggact gtggtcgctc gcattgccat
    12481 gtacccgatt tagtgtaagc ggcggcgtat ccgctgtcta tctcgcgcgg atcgacgcca
    12541 atttgatttc aagcgatatc ggttgcggta gcttattggc gcggtggcag cgggagcggt
    12601 gagagaacag gctggagcgg cggggctggc aggcacctat gagcggcacg ccgagaacgt
    12661 tcgccgggcc gcctactccg tcacgcacga ccgcgccctg gccgaggacg tgacccagga
    12721 tgtgttcctc gctctctggg cacggccgga gcgctacgac cccgcgcgtg gcagcgtgga
    12781 gaacctcctg cgcgtgatgg cccgcagccg cgcgctcgac gcggcgcggc gcaactccgc
    12841 ggcgcggcgc gcgcaggacc ggctcgacgc ggaaccggtc gcggcctcgg agcccgaccc
    12901 cgccgaggcc gcggccacct ccctgcgcgc ggggcggctg cgccgggccg tgcagggcct
    12961 accccccgag cagcgcgacg cgatcgcgct cgcgtactgg ggtggcctga ccgcggagga
    13021 ggtcgcgcac cggcgcggca tcccgcacgg cacggccaag agccggatcc ggatcgggct
    13081 caagaagctg cgcagcgact tcgacggctg acgggctggc cgggcggcgg ccgatcgagc
    13141 cgagtggtgc cgtgtgggag cattccggga tggccaaggc cccgccggga gctccacccg
    13201 gccccccgct cccccgggtc gtccagacgc tcggctttct gaccgccggc acccgctacc
    13261 tcgaggcctg ccggcgccgc tacggcgacg tcgtgtggtt ccggacgctg ttcgaccccg
    13321 gcttcgcgat ggtcttcgac ccggacgtgg tccgggacgt gttccaggcg ccgcccgacc
    13381 ggctgcgcgc gggcgaggcc aacgcgctgc tgtcgacggt cctggggccg cgctcggttc
    13441 tcgtgctcga cggggccgag cacctgcgcc accgcaagct gatgctgccg ccgttccacg
    13501 ggcgccggct caccgcccac gaggaggtga tgcgcgagag cgccgatcgc tcgatcgacg
    13561 cgtggccggt cggcggcgag ttcgcgctgc tgccgcacat gtacgtgctc accctcgacg
    13621 tgatcgccac cgcggtcttc ggcatcgagg aggacgaccg ccgcgaggag ctcaagcggc
    13681 gcatccggac cttcctcgag acggcgggca gcccgctgcg cgtgatcctg ctgatgctct
    13741 cgcgcgggcg gatccgcgac gaggctgcgc agcgccagtt cgacgctcgc cgcgaggcca
    13801 tggacgagct gatcctcgac gagatcgcgc agcgccgcgg cgcacccgac atcgaggagc
    13861 gcgacgacgc gctctcgctg ctcctccttg cgcgggacga ggacggggcc gggctgaccg
    13921 acgcggaggt gcgcgaccag ctcgtgacgc tgctcgttgc cggccacgag accacggcgg
    13981 ccgggatcgc gtggacgctc gacctggtgt accgcgaccc ggccgtgctg gagcgtgccc
    14041 gcacggccgc gcgcgagggc gagaccgtct acctcgacgc ggtcgtgaag gagtcgctgc
    14101 gcatacgccc cgtcgtgccc gggatcggcc gcgtggtgcg cgaggagccg ttcgtggccg
    14161 gcggctactc gatcccgcca ggggtggaga tcaacccgtc gatc
//
LOCUS       DASVPL010000261        22867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_30348, whole genome shotgun sequence.
ACCESSION   DASVPL010000261 DASVPL010000000
VERSION     DASVPL010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22867
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30348"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /gene="dnaK"
                     /locus_tag="VF517_14260"
     CDS             complement(<1..1265)
                     /gene="dnaK"
                     /locus_tag="VF517_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX8054151.1"
                     /translation="MGRTIGIDLGTTNSCMAVLEGGDPTVVENAEGARTTPSVVAFTS
                     GGERLVGTVAKRQAVTNPENTVFSIKRFMGRKAAEVKEEETIVPFKVVKGPNGDARVE
                     AGGAQYSPPEISAMILQKLKTDAEAFLGETVDSAVVTVPAYFNDDQRQATKDAGKVAG
                     LDVKRIINEPTAAALAYGLDKEADQTILVFDLGGGTFDVSVLEIAEGVFEVKATAGDN
                     HLGGDNFDKAIVDWLVKEFKASQGIDLSQDKMALQRLYEAAEKAKVELSTTQESQINL
                     PFVTADASGPKHLDQRLTRSKLTELTADLLDRTVGPTKQAMKDAGVTGDQIDHIVLVG
                     GMTRMPSVQEKVKELTGKDPHKGVNPDEVVAVGAAIQAGVLAGDVKDVLLLDVTPLTL
                     GIETKGGVMTKLIERNTTIPTRKSEVFSTA"
     gene            1466..2593
                     /locus_tag="VF517_14265"
     CDS             1466..2593
                     /locus_tag="VF517_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054152.1"
                     /translation="MELSGGTDLRRLRSLKDQHKAELLALPNVIGVGLGQRVIAGTRT
                     EEWVISVNVVRKLAPDELDRDARIPAELDAEIRGTHVTAKTDVVESGVPRLEGYKDRI
                     RPVQPGYSIRPYESNYLGTTGGFVVVEGDGYLMSNFHVLWDYLPGSPIVQPGDTSDPT
                     DVIGNPDTRHYVQPSPDKVNYMDAALATLKEIDGNYRHIQPAHPEFGPLAGSYNSVGM
                     GWHMRKVGATTGLTEARVSKWDFGGGFDSNHDGVADVWFEHQVELWNASAMPSDGGDS
                     GAWWVTDDNRVGLLHWAGNGVDLSVATPISWVLQKFGASVWAKWGRDRELITGDAQPA
                     DGPSLDDLYDPDELEHIIHSKEADRPAAPAMGGVAAASTDA"
     gene            complement(2617..3492)
                     /locus_tag="VF517_14270"
     CDS             complement(2617..3492)
                     /locus_tag="VF517_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8054153.1"
                     /translation="MSRQRVSELGLVGIAAVWGLTFPMVQDAVEELPTMAFLGYRFLA
                     AALVVALVFRRQLAALSRAGWLAGAVMGVFLTGGYVFQTLGLERTSSSNAGFITGLFV
                     VLTPLLGALVLRQRIDALAWAAAAVSAAGLYLLSGTEGFNAGDALVLGCAISFSAHIL
                     ATGRAATEHDAGALLVVQLAVCGIFCTAVAGGGGQLEVPTGGDVWLALAVTAFVASAL
                     GFFVQTYAQQHAAPARTALILASEPAFAGLFGFLLNDERLSVVAWSGALLIMAAIVAV
                     ELIPRWRPPRPLPEA"
     gene            complement(3489..4175)
                     /gene="nth"
                     /locus_tag="VF517_14275"
     CDS             complement(3489..4175)
                     /gene="nth"
                     /locus_tag="VF517_14275"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX8054154.1"
                     /translation="MPSRAIARPATATPRRFRTILERLREAYGRPLPLEHRAPVDELV
                     LTVLSQNTNDRNRDVAYTRLRERFATWDEVREAPVAEVEDAIRPGGLAPTKAVRIQEI
                     LRAIGDDDLSWLAEAPLAEGRDYLCALPGVGRKTAACVLLFSYGRPDVPVDTHVYRVG
                     SRLGLWRAGAPLDEAHDEMLRLAAKEDPYEAHVALIRHGRRICHARSPRCRECPLLSL
                     CPYGKALVRG"
     gene            4194..4997
                     /locus_tag="VF517_14280"
     CDS             4194..4997
                     /locus_tag="VF517_14280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8054155.1"
                     /translation="MTEHSAEIAGTHVAWVEHDGPSPVLYVHGVPNHGEMWTPFLERT
                     GGVAPDLPGFGRSGKPANFEYSIKGYGDFLEAFVDHLGLERFALAMHDWGSVGLELAQ
                     RRPEAVERLVLLDVVPFLPGYRWHWVARTWRTPVAGELLMGSSTKWATRRLLRTTGAI
                     PEEHVPAFVEEMWPHFDHGTQRAILKLYRSAPPAVLEQAGARLADITAPALVVWGEDD
                     VYLPTRFAHAYAEALGGETEVEVLPDALHWVWLDRPDVVDRVSAFLGAR"
     gene            5008..6684
                     /locus_tag="VF517_14285"
     CDS             5008..6684
                     /locus_tag="VF517_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054156.1"
                     /translation="MPSSAPATKPAPAATRDRSANGGPPGTLAEADRRKALAWRLAPV
                     ALAALASLAYLIAEPRTVDFAAGLYRTHLFSEEGFALWNGNWYSGHHVLGYSVLFPPL
                     AWLVGPRLLGVICALAGAALFEALVRRHFGERARWGALFFGLAAVAPLWSGRNPFLLG
                     ATIGLAAALALQRDRRGLAVGLAAACGLASPVAGAFLGLVTLAYGLSARARRAPAAWI
                     LAAAMGPAVLIGLAFGTDGREPFDLSTFWPLPLLAAGFAVALPREQRTLRIAALLYAL
                     ACCAAYVVDSPMGGNAARLAGFIGAPVLLCALLAYGRPRQRAALAAAAVTLVGLLFIQ
                     WSPPIRDTVKAFEDPSAEPSYYKPLNAFLASAGGPPGRVEIPFTRSHWEAYDVGREHP
                     LARGWLRQADIQRNGIFYGGAFDSLTYAAWLSEHGVRWVALPDAKPDYSSYDERALIE
                     AGLPYLKLRWRSDHWRVYEVTLPNAMVVPRGGARLEIERLGNDDVELRALGRGEATVR
                     VAWSHYWKADSGCVERDGEWTRVIADRAGRIRLSMDFAPSRIVSRGRRCA"
     gene            6725..7501
                     /locus_tag="VF517_14290"
     CDS             6725..7501
                     /locus_tag="VF517_14290"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8054157.1"
                     /translation="MTRAWYLSSRWLPQGWLDALRQLVLFAGAYYAYRIVRGFVDGQT
                     AVAFQHARDIVDAERAMGLFFEPSFQDAVVSQTWLRDFVNFMYVNSHFMITTTFLIWL
                     YLARNHAFYYVRNMFMIAMGLALVGYLVYPTAPPRLLPEWGFTDTVAEAVGPAVENTS
                     EVLYNPYAAMPSMHVAFALMISIPAMKLVGNQVLRYVWAAYPAIVTFVVVTTGNHFWI
                     DAAVGAAVAAVSAYTAHAALGRARPEAWAWRSAVPAKAAA"
     gene            7540..8157
                     /locus_tag="VF517_14295"
     CDS             7540..8157
                     /locus_tag="VF517_14295"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8054158.1"
                     /translation="MRTVEDARTLARNRLIESRLTPNAISMAGLVGNVVAAVLITQRY
                     FFLAGVAFVLGSVMDTLDGRYSRMSGKGSAFGAFLDSTLDRIEEGVVLAACAAYFSDR
                     GDDLAVAAVVITVLGSLMVSYTRARAEALGVECKVGLATRPVRVVILSAGLLFARGAS
                     LGDFELLAPAIYVMAVLTSFTVLQRVLHVRAALNRADQGLSAPSA"
     gene            8244..9407
                     /locus_tag="VF517_14300"
     CDS             8244..9407
                     /locus_tag="VF517_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX8054159.1"
                     /translation="MTQIPGNGASRYQTEKVRVAIVGVGNCANSFVQGVQYYRDATGS
                     EAVPGLMHVDLGGYHVGDIEFTAAFDIDADKVGKDLSEAIWSGQNNTIKFADVPKLDV
                     PVHRGMTHDGLGKYLKEKITKAPGETADIVQILKDTKTDVVVSYLPVGSEQATKWYVE
                     QVLEAGCGFVNCIPVFIAREEYWGNRFRKAGLPIVGDDIKSQVGATIVHRTMARLFGD
                     RGVKMMRTSQLNVGGNMDFYNMLERERLESKKISKTNAVTSIMDHELPADDVYIGPSD
                     YVPWLTDRKWAHIRVEGQAFGDVPLNMEMKLEVWDSPNSAGIVIDAVRCCKLALNNGL
                     GGPLEAPSSYLMKSPPVQVPDPVARQNTEDFIAEYGGAPKLPGQAKIRAVEPA"
     gene            9451..11325
                     /locus_tag="VF517_14305"
     CDS             9451..11325
                     /locus_tag="VF517_14305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8054160.1"
                     /translation="MPDERFSIAQVTPYAWEQPHDVNRFVERLSDELCARGHRIVVVA
                     PSDSRELIRESRETIRRCRDEPECVFAGEGCANVLAVGQSIPFPPGVRRGGSVSLPAN
                     VSRSIEDLFDNAQFDFVHVHEPFAPSAASAALRHSRALNVGSFHGGTERILSTQVARK
                     LVTLLFGRLDGRTTSSAATRDLIGRFFPGDYRVVSPGAEVVARPPGDRDREIVDILYC
                     AEEERGALRLFLRALRRIPPELPWHATVWSRSADAVPAVPLPRSIRDRVRFVGPAQGS
                     EAQHVVRADVVVAASAGAAPATHLPLWAIAGGAVPVASRLPQYEEALGDGDLGLLFEP
                     RDTDTLAAQLARLVQERELRERLRDRVVEAQPGLSWSRVADEFEDLYRMIAARRHDPA
                     PKAAVRKRLEGRDQIHVDLHMHTNHSHDCATPVDTLLEGAKAAGLGAIAVTDHNEISG
                     ALEARERADGIKVIVAEEIKTADQGEVIGLFIEEKIPRGLTLQETIAEIRRQGGLVYV
                     PHPFDRMHSVPDYEHLLDVIEDVDAIEVFNPRVAISSFNEEAERFAAKYRIVAGAGSD
                     SHVVQGLGSVKIVMRDFDGPEEFLESLRAADIVRRRQSLLYVQAVKLIQKRGHGAGKR
                     "
     gene            11373..11978
                     /locus_tag="VF517_14310"
     CDS             11373..11978
                     /locus_tag="VF517_14310"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX8054161.1"
                     /translation="MPATDDEIRQKYLDRAIRELNQLTHEIQECDLCPRGQVMPVLPS
                     GHCLGDIFLLKYVPAPSEISEGVAFYGRAGQALKKSFARLGIDPSVVYGTVFVKCPVG
                     DTEDADPACRARVLEELAIVQPRIVVIMGDEALAELNEIGVPLGRRVEPLVGEIQKLT
                     PACDALFVPDIDRALDDESAKREFWKAFRSLGDWYADFPPY"
     gene            12013..12195
                     /locus_tag="VF517_14315"
     CDS             12013..12195
                     /locus_tag="VF517_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054162.1"
                     /translation="MDLEPKSVDPVIERTCAECGAKLTEQEIRAAMEAGQTYLCSVHA
                     AELEPAEDQAELDEPG"
     gene            complement(12208..13584)
                     /gene="rlmD"
                     /locus_tag="VF517_14320"
     CDS             complement(12208..13584)
                     /gene="rlmD"
                     /locus_tag="VF517_14320"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX8054163.1"
                     /translation="MSGTPTARSARPQRGAELELTVDSFANGGNGIARHEGYVLFVQG
                     GVPGDRLRARVTKAKRDYGEARAIELIEPSPDRIEPRAAHPGASWQVLPYERQLEEKE
                     RQVREALERIGGFESPPLEPMVPAIEPWRYRNKVEYSFGTADDGGLVLGFHRPGRWNE
                     IDDVTDDILASERVDAVRERVKAWCRAEGLDAYDRRAGDGFLRNLVVREGRRAGMLQA
                     RVVTSEGDFRADELAEAAGADGFLWTRTGGVAETTREGKTRLVSGAGAIEEEIDVLGD
                     RLRFRVSPEAFFQTNTEMAERLYGAAVELAGLGGSERVYDLFSGIGTIALAMATRAGE
                     VIGVELVERAVADAIENAKLNGIDNARFFAGDVRTAMRPLVEEAGKPDVVVVDPPRAG
                     LSAKIVRRLLETEARRVVYVSCNPTTLAPNARQIADAGYRLAAVRPVDMFPQTPHIEC
                     VALLERTA"
     gene            13606..15051
                     /locus_tag="VF517_14325"
     CDS             13606..15051
                     /locus_tag="VF517_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054164.1"
                     /translation="MNLDAYRADAQAFWQELEREYLLHLSGRKEGFELEAIYAGHAGL
                     FTREAVERLREDDAPRELVRFAVEGLLGQATKGEAEEVARVETAARVHANGDELGFRE
                     AVAVQANETDADRREAIDRARREVVERDLNPLLLEAHERAAALTRELGWESTLAMCEE
                     LGGIDLRALGRQAEAFVAATEEAYEPMVEPELRRQLGYGLDRLRRADLPAFFRARGFD
                     DGFPGERLLESLERTAGGLGLDGARVQIDAERRPTKSPRAFCAPVRVPDEVHLVIAPV
                     GGCDDYEALMHEAGHAYHYSHTDRRLPFEASCLGDNSVTEAYAFLLQHLTADAEWLAA
                     MLGMADSEPVLRFARASRIVFLRRYCAKLGYELGLHADGRPPAEHRAEYSRRLGTALR
                     VDWPGETWLADVDSFFYAARYLRAWAFETRLRLLFQERFGPRWFAEPEAGALLRSLWA
                     TGQARSADELLAELTGERLDLGVLATDLAVA"
     gene            complement(15048..15974)
                     /locus_tag="VF517_14330"
     CDS             complement(15048..15974)
                     /locus_tag="VF517_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054165.1"
                     /translation="MADSGSSGGGSSRKSGGSRSKSSSSRAKSSGGSRAKSSGGSRST
                     SGSGRAKSSSSGSASGSSRAKSTSSRAKSTSSRSSSSSSSRSGSTSSRSGGQSKRRAA
                     AKKGGQARARQQKAQKAGRQATQAAEAATGTEFSGKSVAELRDALTKGVVAPLNIVML
                     SRERIEEVVDDAVKRGRMTADDAQELVQGLVQRGRRQTNDVLRDLEQLLGRGGEVVEG
                     ARKRGSTAAKRGRSRVRDVADPVIAQADRARRVAGVGPNFPILGYDDLTAAQVQTRLE
                     GLTAAELRKVRDYERRNANRKSVLDAIESKLS"
     gene            complement(16015..16842)
                     /locus_tag="VF517_14335"
     CDS             complement(16015..16842)
                     /locus_tag="VF517_14335"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8054166.1"
                     /translation="MTRHPGIVVRALTWNLFHGRDHPPDPALLTWRSRILRTPERGDT
                     HAQVNRRLDREFHRFVAGLEWDVALLQEAPPRWLRPLCTEAGASGASALTSRNQLALA
                     RSALARLNPDLIASNEGGSNQVLARPPWRIVEVRRHTLALLPERRRMIWTLLARPDGA
                     ALAVANLHASTGGRQPTRAELRDAAAHAVEWSGDTPLVFGGDLNIRPRRAPHEFERLR
                     TRFGLRGDTPRDAIDHLLARNARPLGPPVARHAELPVDGRLLRLSDHAYVTAAFEVE"
     gene            16831..17727
                     /gene="argF"
                     /locus_tag="VF517_14340"
     CDS             16831..17727
                     /gene="argF"
                     /locus_tag="VF517_14340"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX8054167.1"
                     /translation="MTRHFLTGEELTSEELGSLLDRAIELKAARAAGGSSWEALGGRS
                     VALVFEKPSTRTRVSFEVGIHELGGHPVVLRESETQLSRGESLRDFALVLSRMVAAIG
                     VRTGPHSTLEELARHASIPVVNMLTADHHPCQALADLQTLRERFGDLRGLKLAYVGDG
                     NNVTHSLMLVGALAGVEVVAATPPELRPDAAIVEASGATVTDDSAAAAEGAHALYTDV
                     WVSMGDADSEAKRELLAPYRLDEALMDRARDDAIALHCLPAHPGEEISEGVLYGERSA
                     VWDQAENRLHAQKALLELLLSA"
     gene            complement(17724..18206)
                     /locus_tag="VF517_14345"
     CDS             complement(17724..18206)
                     /locus_tag="VF517_14345"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8054168.1"
                     /translation="MSESLVEGLALRWRTAWTEGTRTSFAACCSAGVQYEDPVAREPL
                     EGLDALAEHATMLRDALPDLRIESVGQAVNEGSFACVPWKLLGTHRGSIAAIPASNRF
                     VVVQGVHYLEVADGRVRRARGFFDLYDAAIQLGLLPGRGSLAESALMLVRGFGLRARA
                     "
     gene            complement(18203..18970)
                     /locus_tag="VF517_14350"
     CDS             complement(18203..18970)
                     /locus_tag="VF517_14350"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX8054169.1"
                     /translation="MSLTIAGRTFRSRLIMGTGGFRNLETMAEAVMASGTELATLALR
                     RVDPGARGSIVEALEQAGCALLPNTAGCYTARDAVTTAQLAREAFETDWVKLEVIGDD
                     RTLLPDPTELLDAAETLVADGFTVLPYTNDDPILARRLEDAGCAAVMPLGSPIGSGMG
                     IRNPYNLRLIVEQARVPVILDAGIGTASDAALAMEAGCEGVLLASAVSRAEDPVRMAR
                     AMRLAVESGRLAFESGRIPRRLYAEASTPAEGIPELT"
     gene            complement(18967..19173)
                     /gene="thiS"
                     /locus_tag="VF517_14355"
     CDS             complement(18967..19173)
                     /gene="thiS"
                     /locus_tag="VF517_14355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX8054170.1"
                     /translation="MNLVLNGQPRELPDGAKVSDAVSASGIPALARGIAVAVDREVVP
                     RSLWEATPLADGQRVEVLEAVQGG"
     gene            complement(19170..20351)
                     /gene="thiO"
                     /locus_tag="VF517_14360"
     CDS             complement(19170..20351)
                     /gene="thiO"
                     /locus_tag="VF517_14360"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX8054171.1"
                     /translation="MADAPRAREYDAAVVGAGIVGLASAWRLAQRGLSVVAIDRGAAG
                     SGASSVAAGMLAPVTEAEFGEQALLGLNLAGAAAWPGFAAELAERSGLGTGYRECGAL
                     VVAVDRDDLEEIRRLHSFQRSLGLDAEWLTGRECRRLEPGLAPSVGGGVLAAHDHQAD
                     PRATLAALRTAFEHEGGEIVEGVEVTGVEDHSLVTSAGHVHAEHVVVAAGAWSAALAP
                     DAPPLRPVKGQVLRLREGAGSGPGGAAPSGGAAPTGGPPALASRIVRTPRCYVVPRAG
                     GEIVIGATVEERGFDPAVTAGGVHRLLEAAWEALPDVEERELVETSASFRPATPDNAP
                     VVGRGSTAGLIWATGHHRNGILLAPITASAVAALATGAEPVEELAGFGPERFALAGRH
                     A"
     regulatory      complement(20376..20485)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20514..20732)
                     /locus_tag="VF517_14365"
     CDS             complement(20514..20732)
                     /locus_tag="VF517_14365"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HEX8054172.1"
                     /translation="MTIGTSIVLIAIGAILKYAVNADVDAIDIQVVGTILMIVGILGL
                     VISLLYTFVLADRRRPDPYDAPTRRDRF"
     gene            20752..21429
                     /gene="thiE"
                     /locus_tag="VF517_14370"
     CDS             20752..21429
                     /gene="thiE"
                     /locus_tag="VF517_14370"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX8054173.1"
                     /translation="MARRDHAERIRHASLYLILEVAAAHAVEPALEGGVDVVQIRDKH
                     APDGELVAAGHELGDLCRRHGALFVVNDRPDLALECGADGVHVGQDDESVDAVRALAG
                     DRLFVGVSTHSPAQVAAAEESDADYFAVGPVHATPTKPGRPAVGLELVRHAAEHARKP
                     WFAIGGIDAGNAAAVVAAGARRLAVVRAIRDADDPRAAARELRAIASGATGATGAAAA
                     ARGGVRG"
     gene            21422..21937
                     /locus_tag="VF517_14375"
     CDS             21422..21937
                     /locus_tag="VF517_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054174.1"
                     /translation="MGKRSRKRPSRPPAPERRDTNGTQPAKKLSRSEAKDAAARAALV
                     PLAEGERPRAVTVGAVLAGLFAVGNLIAYAAGIEINGERPQAGGVIAYSLLMGVAAWG
                     MWRSKYWAVLGLQAILGILIVIFSLFLFEAENLKSGLICVAVIAPAGLLFFHLVKAMA
                     RIQMPDRRPSS"
     gene            21934..22350
                     /locus_tag="VF517_14380"
     CDS             21934..22350
                     /locus_tag="VF517_14380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8054175.1"
                     /translation="MSGDFSIVQRVRFGDLDAMQHMNNVEFLRFFETARIDYITQLAP
                     EHNPGNPNGFGFIFAECHIAYRAPAHYNEEIRTFIRPVELKRSSLKLEFEMHVDADDR
                     LVAEGWGTLVGYDYESGRAHPIPDAFRERVEPLLGR"
     gene            22363..>22867
                     /locus_tag="VF517_14385"
     CDS             22363..>22867
                     /locus_tag="VF517_14385"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8054176.1"
                     /translation="MPESSYDCIVIGSGPGGYVAAIRAAQLGMKTAVVEKDRVGGRCL
                     NYACIPAKAVLRAADIKTEVEHGSSMGIVAENVSVDFDGVVKHREKVVKTLTGGVSGL
                     FKKTKIDLIEGFGALTEDANVRIGAAKDGDEIEAGKVILATGSIPKPLLGLQFGGRVL
                     GTETMWAI"
BASE COUNT         3102 a         8227 c         8335 g         3203 t
ORIGIN      
        1 gccgtcgaga acacctccga cttgcgcgtc gggatcgtgg tgttgcgctc gatgagcttc
       61 gtcatcacgc cgcccttggt ctcgatgccg agggtcagcg gggtcacgtc gagcaggagg
      121 acgtccttga cgtcgccggc cagcacgccc gcctggatgg cggcgcccac tgcgacgacc
      181 tcgtccgggt tgacgccctt gtgcgggtcc ttgccggtca gctccttgac cttctcctgc
      241 accgacggca tgcgcgtcat gccgccgacc agcacgatgt ggtcgatctg gtcgccggtc
      301 acgccggcgt ccttcatcgc ctgcttggtc ggcccgaccg tgcggtcgag caggtcggcc
      361 gtcagctcgg tgagcttcga gcgcgtgagc cgctggtcga ggtgcttcgg ccccgacgcg
      421 tcggccgtga cgaacggcag gttgatctgc gactcctgcg tggtcgagag ctcgaccttg
      481 gccttctcgg ccgcctcgta gaggcgctgg agggccatct tgtcctgcga gaggtcgatg
      541 ccctgcgagg ccttgaactc cttgacgagc cagtcgacga tcgccttgtc gaagttgtcg
      601 ccgccgaggt ggttgtcacc cgcggtggcc ttcacctcga acacgccctc ggcgatctcg
      661 agcaccgaca cgtcgaacgt gccgccgccg aggtcgaaga cgaggatcgt ctggtcggcc
      721 tccttgtcga gcccgtaggc gagcgcggcc gcggtcggct cgttgatgat ccgcttgacg
      781 tcgaggcccg cgaccttgcc cgcgtccttg gtggcctggc gctggtcgtc gttgaagtac
      841 gccggcacgg tgaccacggc gctgtcgacc gtctcgccca ggaaggcctc ggcgtcggtc
      901 ttcagcttct ggaggatcat cgcgctgatc tccggcgggc tgtactgcgc gccgccggcc
      961 tccacgcgcg cgtcgccgtt cgggcccttg acgaccttga acggcacgat cgtctcctcc
     1021 tccttcacct cggccgcctt gcggcccatg aagcgcttga tggagaagac ggtgttctcg
     1081 gggttggtga cggcctgccg cttggcgacc gtgccgacca ggcgctcgcc gccgctcgtg
     1141 aacgcgacta cggacggcgt cgtgcgcgcg ccctcggcgt tctcgacgac ggtcggatcg
     1201 ccgccctcga ggaccgccat gcacgagttc gtcgtgccca ggtcgatgcc aatcgttctg
     1261 cccatagggg ttcttgctcc ttctcggttc taaatcgcga cgctaagaaa tctgagtgtg
     1321 gttttagcag atcgtgatcg aggcgcccac cggcggcgac ggtccgtacc gctcagtcgg
     1381 cgctgcgcgc ttgacagcgc atcgcccccc ggcttacgtt cggcacgagc cttcggggga
     1441 gacgcgacga cgggttgggg gaagcatgga gctgagtggg gggaccgatc tgagacggct
     1501 gcggtcgctc aaggaccagc acaaggcgga gctgctcgcg ctgccgaacg tgatcggggt
     1561 tggactcggt caacgcgtga tcgcgggcac gcgaaccgag gagtgggtaa tcagcgtcaa
     1621 cgtcgtgagg aagctcgccc cggacgagct cgaccgcgac gcgaggattc ccgccgagct
     1681 ggacgccgag atccggggca cgcatgtgac ggcgaagacg gatgtggtcg agagcggcgt
     1741 cccccggctc gagggctaca aggatcgcat caggccggtt caaccgggtt actcgatccg
     1801 gccctacgag tccaactatc tgggcacgac cggcgggttc gtcgtggtcg aaggcgacgg
     1861 gtacctcatg agcaacttcc acgtgctctg ggactacctg ccgggctcgc ccatcgtcca
     1921 gcccggggac acgagcgacc ctacggacgt catcgggaac cccgatacga ggcactacgt
     1981 ccaaccctcg cccgacaagg tgaactacat ggacgccgcc ctcgcgacgc tgaaggagat
     2041 cgacggcaac taccgccaca tacagcccgc gcacccagag ttcggcccgc tcgccggaag
     2101 ctacaacagc gttggcatgg ggtggcacat gcgcaaggtc ggcgcgacca ccggcctgac
     2161 ggaggcgcgg gtcagcaagt gggacttcgg cggaggcttc gactcgaacc acgacggcgt
     2221 cgcagacgtc tggttcgagc accaggtcga gctctggaac gcctcggcca tgccgtcgga
     2281 cggcggcgac tcgggcgcct ggtgggtcac cgacgacaat cgtgtcgggc tgctccactg
     2341 ggccggcaac ggcgtggacc tgtccgtcgc caccccgatc tcgtgggtcc tccagaagtt
     2401 cggcgcgtcg gtctgggcga agtggggtcg cgaccgggag ctgatcaccg gcgacgccca
     2461 gccagccgac gggccctcac tggacgacct gtatgacccc gacgagctcg agcacatcat
     2521 ccattcgaag gaagcggacc gcccggcggc gccggccatg gggggagtgg cggcggccag
     2581 cacggacgcg tagacctgtc cgcgcacgca cggccgtcaa gcctccggca gcggccttgg
     2641 cgggcgccag cgcgggatca gctcgacggc cacgatcgcg gccatgatca ggagcgctcc
     2701 gctccaggcc acgaccgaga gccgctcgtc gttcagcagg aagccgaaca ggcccgcgaa
     2761 cgcgggctcg gacgcgagga tcagtgcggt gcgcgccggc gccgcgtgct gctgcgcgta
     2821 ggtctggacg aagaagccga gcgcgctcgc cacgaacgcc gtgaccgcca gggcgagcca
     2881 cacgtcgccg cccgtgggga cctcgagctg gccgccgcca cccgcgacgg ccgtgcagaa
     2941 gatcccgcac accgcgagct ggacgacgag cagcgccccc gcgtcgtgct cggtcgccgc
     3001 gcggccggtc gcgaggatgt gggcggagaa cgagatcgcg cacccgagca cgagggcgtc
     3061 gcccgcgttg aacccctccg ttcccgacag caggtagagg cccgccgccg agaccgcggc
     3121 cgcggcccac gccagtgcgt cgatccgctg gcgcagcacg agcgccccca ggagcggcgt
     3181 gaggacgacg aacaggccgg tgatgaagcc cgcgttcgag gagctcgtgc gctcgagccc
     3241 gagcgtctgg aagacgtatc ccccggtgag gaagacgccc atgacagcgc ccgccaacca
     3301 gcccgcgcgc gagagcgcgg cgagctgacg gcggaacaca agcgcgacca cgagcgcggc
     3361 cgcgaggaag cggtagccga ggaacgccat cgtcgggagc tcctcgacgg cgtcctggac
     3421 catcgggaac gtcagtcccc agaccgccgc gatgccgacc agcccgagct cggagacgcg
     3481 ctggcggctc acccgcggac gagcgccttc ccgtacgggc agagcgacag cagcgggcac
     3541 tcccggcagc gcggcgagcg cgcgtggcag atccgccgac cgtgccggat cagcgccacg
     3601 tgcgcctcgt acgggtcctc cttcgccgcc agccgcagca tctcgtcgtg tgcctcgtcg
     3661 agcggcgcgc cggcccgcca cagcccgagc cgcgacccga cccggtacac gtgcgtgtcg
     3721 accggaacgt cggggcgccc gtacgagaac agcaggacgc acgcggccgt cttgcgtccc
     3781 acaccgggca gggcgcagag gtagtcgcgc ccctcggcaa ggggcgcctc ggccagccag
     3841 ctcaggtcgt cgtcgccgat cgcgcgcagg atctcctgga tgcgaacggc cttggtgggc
     3901 gcgaggccgc ccgggcggat cgcgtcctcg acctcggcca cgggcgcctc gcgcacctcg
     3961 tcccaggtcg cgaagcgctc gcgcaggcgc gtgtaggcga cgtcgcggtt gcggtcgttc
     4021 gtgttctgcg agagcacggt gagcacgagc tcgtcgaccg gcgcgcggtg ctcgagcggc
     4081 agcggccgcc cgtaggcctc gcgcagtcgc tcgaggatcg tgcggaagcg ccgcggcgtg
     4141 gcggtggcgg gtcgcgcgat ggccctgctc ggcatccgtg gaaaggtacg ggcgtgaccg
     4201 agcacagcgc cgagatcgcc gggacgcacg tcgcctgggt cgagcacgac gggccgagcc
     4261 cggtcctgta cgtccacggc gtgcccaacc acggggagat gtggaccccg ttcctcgagc
     4321 gcaccggcgg cgtcgcgccc gacctgccgg gcttcgggcg ctccggcaag ccggccaact
     4381 tcgagtactc gatcaagggc tacggcgact tcctcgaggc gttcgtggac cacctcgggc
     4441 tcgagcggtt cgcgctcgcg atgcacgact ggggcagcgt cgggctcgag ctcgcgcagc
     4501 ggcgcccgga ggccgtcgag cggctggtgc tgctcgacgt cgtgccgttc ctgccgggct
     4561 atcgctggca ctgggtggcg cggacctggc gcacgccggt cgccggcgag ctgctcatgg
     4621 gctcgtcgac caagtgggcc acccggcggc tcctgcgcac gaccggggcg atcccggagg
     4681 agcacgtccc agcgttcgtc gaggagatgt ggccgcactt cgaccacggc acccagcgcg
     4741 cgatcctcaa gctctaccgc tcggcgccgc ccgccgtgct cgagcaggcg ggcgcgcggc
     4801 tcgcggacat cacggcgccc gcgctcgtgg tctggggcga ggacgacgtc tacctgccga
     4861 cccgcttcgc gcacgcctac gcggaggcgc tcgggggcga gacggaggtc gaggtcctgc
     4921 cggacgcgct gcactgggtc tggctcgacc ggccggacgt cgtcgaccgg gtcagcgcct
     4981 tcctcggcgc tcgttagcct tccgcccgtg ccgtcatccg ctccggcaac caagccggcg
     5041 ccggcggcga cgagggatcg atccgcgaac ggcgggccgc ccggcacgct cgccgaggcg
     5101 gatcgccgca aggcgctcgc ctggcgcctc gccccggtcg ccctcgccgc gctcgcgtcg
     5161 ctcgcgtacc tgatcgccga gccgcgcacg gtcgacttcg ccgccgggct ctaccgcacc
     5221 cacctcttca gcgaggaggg cttcgcgctg tggaacggca actggtacag cggccaccac
     5281 gtgctcggct acagcgtgct gttcccgccg ctcgcgtggc tcgtcggccc gcgcctgctg
     5341 ggcgtgatct gcgcactcgc gggggcggcg ctgttcgagg cgctcgtgcg ccggcacttc
     5401 ggcgagcggg cgcgctgggg cgcgctgttc ttcggcctcg ccgccgtcgc gccgctgtgg
     5461 tcggggcgca acccgttcct gctcggcgcc acgatcggac tggccgccgc actcgcgctc
     5521 cagcgcgatc gccgaggcct cgccgttggc ctggccgccg cctgcggcct tgcgagcccg
     5581 gtcgcgggcg cgttcctcgg gctggtcacg ctggcgtacg ggctgtcggc gcgggcgaga
     5641 cgggccccgg cggcatggat cctcgcggcg gcgatgggcc cggcggtgct gatcgggctg
     5701 gcgttcggca cggacggccg cgagccgttc gacctctcga cgttctggcc gctgcccctc
     5761 ctggccgccg gcttcgcggt cgcgctcccc cgcgagcagc gcaccctgcg gatcgccgca
     5821 ttgctctacg cgctggcctg ctgcgcggcc tacgtggtcg actccccgat gggcggcaac
     5881 gccgcgcgcc tcgccggctt catcggagcc cccgtgctgc tgtgcgcgct gctggcgtac
     5941 ggccggccgc ggcagcgcgc ggcgctcgcc gcggcggccg tcacgctcgt gggcctcctg
     6001 ttcatccagt ggtcaccgcc gatccgcgac accgtcaagg cgttcgagga cccgtcggcc
     6061 gagccgagct actacaagcc gctgaacgcc ttcctcgcaa gcgccggcgg gccgcccggc
     6121 cgggtcgaga tcccgttcac gcgcagccac tgggaggcct acgacgtggg ccgcgagcac
     6181 ccgctcgcgc gcggctggct gcgccaggcg gacatccagc gcaacgggat cttctacggc
     6241 ggcgccttcg acagcctcac ctacgcggcg tggctcagcg agcacggcgt gcgctgggtc
     6301 gcgctgcccg acgctaagcc cgactacagc tcctacgacg agcgcgccct gatcgaggcc
     6361 gggctgccct acctgaagct gcgctggcgc tctgaccact ggcgcgtgta cgaggtcacg
     6421 ctgccgaacg cgatggtcgt cccacggggc ggcgcgcggc tcgagatcga gcggctcggc
     6481 aacgacgacg tggagctgcg ggcgctcggc cgcggcgagg cgaccgtgcg cgtggcctgg
     6541 tcgcactact ggaaggcgga ctcgggctgc gtcgagcgcg acggcgagtg gacgcgtgtg
     6601 atcgcggatc gggcggggcg gatccggctg tcgatggact tcgcgccgag ccgcatcgtc
     6661 tccaggggcc ggcggtgcgc gtgagtggcg aaggtccggg gggtgccact aggatggcgc
     6721 cgccgtgact cgtgcttggt acttgagcag ccgctggctg ccgcaggggt ggctcgacgc
     6781 cctccgccag ctcgtcctgt tcgccggggc gtactacgcc taccggatcg tccggggctt
     6841 cgtggacggc cagaccgccg tcgcgttcca gcacgcgcgc gacatcgtcg acgccgagcg
     6901 cgcgatgggg ctgttcttcg agcccagctt ccaggacgcg gtcgtctctc agacctggct
     6961 gcgcgacttc gtcaacttca tgtacgtcaa cagccacttc atgatcacca cgacgttcct
     7021 gatctggctc tacctcgcgc gcaaccacgc cttctactac gtgcgcaaca tgttcatgat
     7081 cgccatgggg ctggcgctcg tcggctacct ggtctacccc accgcgccgc cgcggctgct
     7141 gccggagtgg ggcttcaccg acacggtcgc cgaggccgtc ggcccggccg tcgagaacac
     7201 ctccgaggtg ctctacaacc cgtacgcggc catgccgagc atgcacgtcg cgttcgcgct
     7261 gatgatctcg ataccggcca tgaagctcgt cgggaaccag gtgctgcgct acgtctgggc
     7321 ggcctacccg gcgatcgtca ccttcgtcgt ggtcaccacc ggaaaccact tctggatcga
     7381 cgcggcggtc ggcgcagcgg tcgccgcggt gtccgcgtac acggcgcacg cggcgctcgg
     7441 ccgcgcccgt cccgaggcct gggcatggcg cagcgcggtc ccggcgaagg ccgcggcgta
     7501 gccacggcga gccccgacgg cccgcggcgc gcctccgagg tgcgaacggt cgaggacgcg
     7561 cgcacgcttg cgcgcaaccg cctgatcgag tcgcgcctca cgccgaacgc gatctcgatg
     7621 gccggcctcg tcggcaacgt cgtcgcggcc gtgctgatca cccagcgcta cttcttcctc
     7681 gccggggtgg cgttcgtcct cgggtcggtc atggacacgc tcgacggccg ctactcccgg
     7741 atgtcgggca agggcagcgc gttcggcgcc ttcctcgact cgacgctcga ccggatcgag
     7801 gagggtgtcg tgctcgcggc gtgcgccgcg tacttctcgg accgcggcga cgacctcgcc
     7861 gtggccgccg tcgtgatcac cgtgctgggg tcactgatgg tgagctacac ccgcgctcgc
     7921 gcggaggcgc tcggggtcga gtgcaaggtc ggcctcgcga cccgccccgt gagggtcgtt
     7981 atcctctccg caggtttgct gttcgcgagg ggggcgtcgc tcggcgactt cgagctgctg
     8041 gcaccggcca tctacgtgat ggcggtcttg acgagtttca cggtgctcca gcgggtactc
     8101 catgtccgag cggcgctcaa ccgcgctgac cagggcctgt ccgcaccctc cgcgtaacca
     8161 gcggaaagtg cggggtttga tctagcctgc cgcaccgggc gcgcccggcc gcgtcgcccg
     8221 acccaacagg aggacccaga aacgtgactc agatccctgg aaacggcgca tcgcgctacc
     8281 agaccgagaa ggtacgcgtc gccatcgtcg gcgtcggcaa ctgcgccaac agcttcgtgc
     8341 agggcgtcca gtactaccgc gacgccaccg ggagcgaggc cgtccccggc ctcatgcacg
     8401 tggacctcgg cggctaccac gtcggcgaca tcgagttcac cgccgccttc gacatcgacg
     8461 ccgacaaggt cggcaaggac ctctccgagg cgatctggtc gggccagaac aacacgatca
     8521 agttcgccga cgtgccgaag ctcgacgtcc ccgtgcaccg cggcatgacg cacgacgggc
     8581 tcggcaagta cctcaaggag aagatcacca aggcgcccgg cgagaccgcc gacatcgtcc
     8641 agatcctcaa ggacacgaag accgacgtcg tcgtctccta cctgccggtc gggtccgagc
     8701 aggccaccaa gtggtacgtc gagcaggtgc tcgaggccgg ctgcgggttc gtcaactgca
     8761 tccccgtgtt catcgcccgc gaggagtact ggggcaaccg cttccgcaag gccgggctcc
     8821 cgatcgtcgg cgacgacatc aagtcgcagg tcggcgccac gatcgtgcac cgcacgatgg
     8881 cgcggctgtt cggcgaccgc ggcgtgaaga tgatgcgcac gtcgcagctc aacgtcggcg
     8941 gcaacatgga cttctacaac atgctcgagc gcgagcgcct ggagtccaag aagatctcga
     9001 agaccaacgc ggtcacctcg atcatggacc acgagctgcc ggcggacgac gtctacatcg
     9061 ggccgtcgga ctacgtgccg tggctgaccg accgcaagtg ggcccacatc cgcgtggagg
     9121 gccaggcgtt cggcgacgtg ccgctgaaca tggagatgaa gctcgaggtg tgggactcgc
     9181 cgaactcggc gggcatcgtc atcgacgccg tgcgctgctg caagctcgcg ctgaacaacg
     9241 gcctcggcgg cccgctcgag gcgcccagct cgtacctgat gaagtcgccc ccggtgcagg
     9301 tcccggaccc cgtggcccgg cagaacaccg aggacttcat cgccgagtac ggcggcgctc
     9361 cgaagctgcc cggccaggcg aagatccgcg cggtcgagcc ggcgtagccg ctagctcggc
     9421 ccgcacggcg cgggggcgta tcctcgcgcc gtgcccgacg agcgcttctc gatagcccag
     9481 gtcaccccct acgcctggga gcagccgcac gacgtcaacc ggttcgtgga gcggctctcg
     9541 gatgagctgt gcgcgcgcgg gcaccggatc gtggtggtcg cgccgtcgga ctcgcgcgag
     9601 ctgatccgcg agtcgcggga gacgatcagg cgctgccgcg acgagccgga gtgcgtgttc
     9661 gcgggcgagg gctgcgcgaa cgtgctcgcg gtcgggcaga gcatcccgtt cccgcccggc
     9721 gtgcgccgcg gcgggtcggt gtcgctgccg gcgaacgtct cgcgctcgat cgaggacctg
     9781 ttcgacaacg cgcagttcga cttcgtccac gtgcacgagc cgttcgcgcc gagcgccgcg
     9841 tcggccgcgc tgcgccactc gcgggcgctc aatgtgggca gcttccacgg cggcaccgag
     9901 cggatcctct cgacgcaggt ggcgcgcaag ctggtcaccc tgctgttcgg ccgcctcgac
     9961 gggcgcacga cgagctcggc ggccacgcgc gacctgatcg ggcgcttctt ccccggcgac
    10021 taccgcgtgg tgagccccgg cgccgaggtg gtcgcgcgcc cgcccggcga ccgtgaccgc
    10081 gagatcgtcg acatcctcta ctgcgccgag gaggagcggg gggcgctgcg gctgttcctg
    10141 cgcgcgctcc ggcggatccc gccggagctg ccgtggcacg ccacggtctg gtcgcgctcg
    10201 gcggacgccg tgcccgcggt gccgctgccg cgctcgatcc gcgaccgcgt gcgcttcgtc
    10261 ggcccggcgc agggttcgga ggcccagcac gtggtgcgcg cggacgtggt ggtggccgcc
    10321 tctgccggag cggcgccggc cacgcacctg ccgctgtggg cgatcgccgg cggcgccgtg
    10381 ccggtcgcct cgcggctgcc tcagtacgag gaggcgctcg gcgacggcga cctcggcctg
    10441 ctcttcgagc cgcgcgacac cgacacgctg gccgcccagc tcgcccggct ggtgcaggaa
    10501 cgtgagctgc gcgagcgcct gcgcgatcgg gtggtcgagg cccagcccgg gctctcctgg
    10561 tcgcgcgtgg ccgacgagtt cgaggacctc taccggatga tcgccgcccg gcgccacgac
    10621 ccggcgccga aggcggcggt caggaagcgc ctcgagggcc gcgaccagat ccacgtggac
    10681 ctccacatgc acacgaacca ctcgcacgac tgcgcgacgc cggtggacac gctgctcgag
    10741 ggggcgaagg cggccgggct gggcgcgatc gcggtgaccg accacaacga gatctccggt
    10801 gcgctcgagg cgcgcgagcg ggccgacggg atcaaggtga tcgtggccga ggagatcaag
    10861 accgccgacc agggcgaggt gatcggcctg ttcatcgagg agaagatccc gcgcgggctg
    10921 acgctccagg agacgatcgc cgagatccgc cgccagggcg gcctcgtgta cgtgccgcac
    10981 ccgttcgacc ggatgcactc ggtccccgac tacgagcacc tgctcgacgt gatcgaggac
    11041 gtggacgcca tcgaggtctt caacccgcgg gtggccatct cgtcgttcaa cgaggaggcc
    11101 gagcgcttcg cggccaagta ccggatcgtc gcgggggccg ggtccgacag ccacgtggtc
    11161 caggggctcg gctcggtgaa gatcgtgatg cgggacttcg acgggcccga ggagttcctc
    11221 gagtcgctgc gggcggccga catcgtccgg cggcggcaga gcctcctcta cgtgcaggcg
    11281 gtgaagctga tccagaaacg agggcacggg gcaggcaaga ggtgatgcag cacaaaggag
    11341 ggcgccccga ccaggcgaaa tcgaccgcct ggatgccagc caccgacgac gagatccggc
    11401 agaagtacct cgaccgtgcg atccgcgagc tcaaccagct cacccacgag atccaggagt
    11461 gcgacctgtg cccgcgcggc caggtcatgc cggtgctccc gtcgggccac tgcctggggg
    11521 acatcttcct cctgaagtac gtgccggcgc cctcggagat ctccgaggga gtggcgttct
    11581 acggccgcgc cggccaggcg ctcaagaaga gctttgcgcg gctggggatc gatccctcgg
    11641 tggtctacgg gaccgtgttc gtgaagtgtc cggtcggtga caccgaggac gccgaccccg
    11701 cctgccgcgc ccgcgtgctg gaggagctcg cgatcgtgca gccgcggatc gtcgtgatca
    11761 tgggcgacga ggcgctggcc gagctgaacg agatcggcgt gccgctcggg cgccgcgtcg
    11821 agccgctcgt gggcgagatc cagaagctca ccccggcgtg cgacgcgctg ttcgtgcccg
    11881 acatcgacag ggcgctcgac gacgagtccg ccaagcgcga gttctggaag gcgttccgct
    11941 cgctcggcga ctggtacgcg gacttcccgc cgtactagcc ggccggcgcg cgcgggtata
    12001 gtcgcccgcc tcatggacct cgagcccaag agcgtcgacc cggtcatcga gcgcacctgc
    12061 gcggagtgcg gggcgaagct gaccgagcag gagatccgcg ccgccatgga ggcggggcag
    12121 acctacctct gctcggtgca cgcggccgag ctcgagccgg ccgaggacca ggccgagctc
    12181 gacgagcccg gctagcgccg agccgagcta ggcggtccgc tccagcagcg ccacgcactc
    12241 gatgtgcggg gtctgcggga acatgtccac cgggcgcacg gcggcgagcc ggtagcccgc
    12301 gtctgcgatc tggcgcgcgt tcggcgcgag cgtggtcggg ttgcaggaga cgtagaccac
    12361 gcggcgcgcc tcggtctcga gcaggcggcg cacgatcttg gccgagaggc cggcgcgcgg
    12421 cgggtcgacc acgaccacgt cgggcttgcc cgcctcctcg accagcggtc gcatggccgt
    12481 gcgcacgtcg cccgcgaaaa agcgcgcgtt gtcgatgccg ttgagcttgg cgttctcgat
    12541 cgcgtcggcc accgctcgct cgaccagctc gacgccgatg acctcgccgg cgcgcgtggc
    12601 catcgcgagc gcgatcgtgc cgatgccgct gaacaggtcg tacacgcgct cgctgccgcc
    12661 gagcccggcc agctcgaccg cggcgccgta caggcgctcg gccatctcgg tgttggtctg
    12721 gaagaacgcc tccggcgaga cgcggaagcg cagccggtcg cccagcacgt cgatctcctc
    12781 ctcgatcgcg ccggcgccgc tcacgaggcg cgtcttgccc tcgcgggtgg tctcggccac
    12841 gccgccggtg cgggtccaga ggaagccgtc ggcgccggcg gcctcggcca gctcgtccgc
    12901 gcggaagtcg ccctcgctcg tgacgacccg cgcttggagc atcccggcgc gcctgccctc
    12961 gcgaacgacg aggttgcgca ggaagccgtc accggcgcgg cggtcgtagg cgtcgaggcc
    13021 ctcggcgcgg caccaggcct tcacgcgctc gcgcacggcg tcgacgcgct cggaggcgag
    13081 gatgtcgtcg gtcacgtcgt cgatctcgtt ccagcggccc ggccggtgga agccgagcac
    13141 gaggccgccg tcgtcggccg tcccgaacga gtactcgacc ttgttgcggt agcgccacgg
    13201 ttcgatcgcg ggcaccatcg gctccagcgg aggcgactcg aagccgccga tgcgctcgag
    13261 cgcctcgcgc acctggcgct ccttctcctc gagctggcgc tcgtagggca gcacctgcca
    13321 ggaggcgccc gggtgggccg cgcgcggctc gatccggtcc gggctcggct cgatcagctc
    13381 gatcgcgcgg gcctcgccgt agtcgcgctt ggccttcgtg acccgcgcgc gcaggcggtc
    13441 gccgggcacg ccgccctgga cgaacagcac gtagccctcg tggcgggcta tgccgttgcc
    13501 cccgttggcg aacgagtcga cggtcagctc gagctccgct ccgcgctggg ggcgtgcgga
    13561 acgggctgtg ggggtgccgg acacgcgacc tatgctggca ggctcgtgaa tctcgacgcc
    13621 taccgcgccg acgcccaggc cttctggcag gagctcgagc gcgagtacct gctccacctg
    13681 tccgggcgca aggagggctt cgagctcgag gcgatctacg cgggtcacgc cgggctgttc
    13741 acgcgcgagg cggtggagcg cctgcgcgag gacgacgccc cgcgcgagct ggtgcgcttc
    13801 gcggtcgagg ggctgctcgg gcaggccacg aagggcgagg ccgaggaggt cgcgcgcgtc
    13861 gagacggccg cgcgggtcca cgcgaacggc gacgagctcg gcttccgcga ggcggttgcc
    13921 gtgcaggcga acgagaccga cgccgaccgg cgcgaggcga tcgaccgggc gcggcgcgag
    13981 gtggtggagc gcgatctcaa cccgctgctg ctcgaggcgc acgagcgcgc ggccgccctc
    14041 acgcgcgagc tgggctggga gagcacgctc gcgatgtgcg aggagctcgg cgggatcgac
    14101 ctgcgcgcgc tcggccgcca ggccgaggcg ttcgtcgcgg ccacggagga ggcgtacgag
    14161 ccgatggtgg agccggagct gcgccggcag ctcggctatg ggctcgaccg gctgcggcga
    14221 gcggacctgc ccgccttctt ccgggcgcgc gggttcgacg acgggttccc gggcgagcgg
    14281 ctgctggagt cgctcgagcg gacggcgggc ggcctcggcc tcgacggcgc gcgcgtgcag
    14341 atcgacgccg agcgccggcc cacgaagtca ccgcgcgcgt tctgcgcgcc cgtgcgcgtg
    14401 ccggacgagg tgcacctggt gatcgccccc gtcggcggct gcgacgacta cgaggcgctc
    14461 atgcacgagg ccggccacgc ctaccactac tcgcacaccg accggcgcct cccgttcgag
    14521 gcgagctgcc tcggcgacaa ctccgtgaca gaggcgtacg cgttcctgct ccagcacctg
    14581 accgccgacg cggaatggct cgcggccatg ctcgggatgg cggactcgga gccggtgctg
    14641 cgtttcgcgc gcgcgagccg gatcgtgttc ctgcgccgct actgcgcgaa gctcggctac
    14701 gagctcggcc tgcacgccga cggccgcccg cccgccgagc accgggcgga gtactcgcgc
    14761 cggctcggca ccgcgctgcg cgtggactgg ccgggggaga cctggctggc cgacgtcgac
    14821 tcgttcttct acgccgcgcg ctacctgcgg gcgtgggcct tcgagacgcg gctgcggctg
    14881 ctcttccagg agcgcttcgg cccgcgctgg ttcgcggagc ccgaggcggg ggcgctgctg
    14941 cggtcgctgt gggccaccgg gcaggcgcgg agcgccgacg agctcctggc ggagctcacc
    15001 ggcgagcgcc tggacctcgg cgtgctggcg accgacctcg cggtcgccta gctcagcttc
    15061 gactcgatcg cgtcgagcac cgacttgcgg ttcgcgttgc ggcgctcgta gtcgcggacc
    15121 ttccgcagct cggcggcggt gagcccctcg agcctggtct gcacctgcgc ggccgtgagg
    15181 tcgtcgtagc cgaggatcgg gaagttcggc ccgacccccg ccacgcgccg cgcccggtcg
    15241 gcctgggcga tcaccgggtc ggcaacgtcg cggacgcgtg accgtccccg cttggccgcc
    15301 gtcgagccgc gcttgcgcgc gccctcgacc acctcgccgc ctcgcccgag cagctgctcg
    15361 aggtcgcgca ggacgtcgtt cgtctgcctg cgcccgcgct gcacgaggcc ctggacgagc
    15421 tcctgcgcgt cgtcggcggt cattcggccg cgcttgacgg cgtcgtcgac gacctcctcg
    15481 atgcgctcgc ggctcagcat cacgatgttg agcggagcga cgacgccctt ggtcagcgcg
    15541 tcgcgcagct cggcgaccga cttgcccgag aactccgtcc cggtcgccgc ctcggcggcc
    15601 tgcgtggcct gccggccggc cttctgagcc ttctgctggc gggcgcgcgc ctggccgccc
    15661 ttcttcgccg cggcgcggcg cttcgactgc ccgccggagc gcgagctggt ggagccggag
    15721 cgactgctgc tgctgctgga cgagcgcgag ctggtcgact tcgcgcggct gctggtcgac
    15781 ttcgcgcggc tgctgccgga cgccgaaccg ctgctgctcg acttcgcgcg gccgctgccg
    15841 gacgtggagc gactgccgcc gctcgacttc gcgcgactgc cgccgctcga cttggcgcgg
    15901 ctgctgctcg acttcgatcg gctgccgccg gacttgcgtg acgacccgcc tcctgagctt
    15961 cccgagtcag ccatgtcgtc tcctctcctc gtattcctgc ggggggtggc tggactattc
    16021 cacttcgaac gcggcggtga cgtacgcgtg gtccgacagg cgcagcagcc ggccgtcgac
    16081 cggcagctcg gcgtggcgcg ctaccggcgg gccgagcggc cgcgcgttgc gcgcgaggag
    16141 gtggtcgatg gcgtcgcgcg gcgtgtcgcc gcggagcccg aagcgggtgc gcaggcgctc
    16201 gaactcgtgc ggcgcgcggc gcgggcggat gttgaggtcg ccgccgaaca cgagcggcgt
    16261 gtcgcccgac cactcgaccg cgtgcgccgc ggcatcgcgc agctcggcgc gcgtgggctg
    16321 gcggccgccg gtgctcgcgt ggaggttggc caccgccagc gcggcgccgt cgggacgagc
    16381 gagcagcgtc cagatcatcc ggcggcgttc gggcaggagc gcgagcgtgt ggcggcgcac
    16441 ctcgacgatg cgccacggcg gccgggcgag cacctggttc gagccgccct cgttcgaggc
    16501 gatcaggtcc gggttcaggc gcgcgagcgc gctgcgggcc agcgcgagct ggttgcgcga
    16561 cgtgagggcg gacgcgccgc tggcccccgc ctcggtgcag agcggtcgca gccagcgcgg
    16621 cggagcctcc tggagcaggg cgacgtccca ctcgagcccg gcgacgaaac ggtggaactc
    16681 gcggtcgagc cggcggttca cctgggcgtg cgtgtcgccg cgctcggggg tgcgcaggat
    16741 ccgcgagcgc caggtgagca gggcggggtc gggcgggtgg tcgcgaccgt ggaagaggtt
    16801 ccaggtgaga gcccggacta cgatccccgg gtgacgcgtc acttcctcac cggcgaggag
    16861 cttacgagcg aggagctcgg ctccctgctc gaccgggcga tcgagctgaa ggcggcccgc
    16921 gccgcgggcg ggtcctcgtg ggaggcgctc ggcgggcgca gcgtcgcact cgtcttcgag
    16981 aagccctcca cacgcactcg ggtctcgttc gaggtcggca tccacgagct cggtggccat
    17041 ccggtcgtgc tgcgggagtc cgagacgcag ctctcgcgag gggagtcgct gcgcgacttc
    17101 gcgctggtgc tctcgcgcat ggtcgccgcg atcggcgtgc gcacggggcc gcactcgacg
    17161 ctcgaggagc tggcccggca cgcgagcatc ccggtggtga acatgctcac cgcggatcac
    17221 cacccgtgcc aggcgctggc cgatctccag acgctgcgcg agcgcttcgg cgacctgcgc
    17281 ggcctgaagc tcgcctacgt gggcgacggc aacaacgtga cccactcgct gatgctggtc
    17341 ggcgcgctgg cgggcgtcga ggtcgtggcc gcgacgccgc cggagctgcg cccggacgcg
    17401 gcgatcgtgg aggcgagcgg cgccacggtg accgacgatt ccgccgccgc cgccgagggc
    17461 gcgcacgcgc tctacaccga cgtctgggtg agcatgggcg acgccgactc ggaggcgaag
    17521 cgcgagctgc tcgccccgta ccgcctcgac gaggcgctga tggaccgcgc gcgcgacgac
    17581 gcgatcgcgc tccactgcct gccggcgcac ccgggcgagg agatctcgga gggcgtgctc
    17641 tacggcgagc gctcggccgt ctgggaccag gcggagaacc gcctgcacgc gcagaaggcg
    17701 ctgctcgagc tgctgctcag cgcctaggcg cgggcgcgca ggccgaagcc gcgcacgagc
    17761 atcagcgccg actccgcgag gctgccgcgc cccggcagca ggccgagctg gatcgcggcg
    17821 tcgtagaggt cgaagaagcc gcgggcgcgg cggacgcggc cgtcggcgac ctcgaggtag
    17881 tggacgccct gcacgaccac gaagcggttg ctcgcgggga tcgcggcgat cgagccgcgg
    17941 tgggtgccga gcagcttcca ggggacgcac gcgaacgatc cctcgttgac cgcctgcccg
    18001 accgactcga tgcgcaggtc cggcagcgcg tcgcgcagca tcgtggcgtg ctcagcgagg
    18061 gcgtcgaggc cctccagcgg ctcgcgggcc acggggtcct cgtactgcac gcccgccgag
    18121 cagcaggcgg cgaacgaggt gcgcgttccc tcggtccacg cggtgcgcca gcgcagggcc
    18181 agcccctcga cgagcgactc gctcacgtga gctccgggat cccctcggcc ggcgtggacg
    18241 cctccgcgta gagccgccgc ggtatgcgtc cggactcgaa cgctagccgc ccgctctcca
    18301 ccgccagccg catcgcgcgc gccatccgca cggggtcctc ggcgcgcgac acggcgctcg
    18361 cgagcagcac gccctcgcag cccgcctcca tcgccagagc ggcgtccgag gccgtgccga
    18421 tgccggcgtc gaggatcacc ggcacgcgcg cctgctccac gatcagtcgc aggttgtacg
    18481 ggttgcggat gcccatgccg ctgccgatcg gcgagccgag cggcatcacg gccgcgcagc
    18541 cggcgtcctc gaggcggcgg gcgaggatcg ggtcgtcgtt ggtgtagggg agcacggtga
    18601 agccgtcggc gacgagcgtc tcggcggcgt cgagcagctc ggtgggatcg gggaggagcg
    18661 tacggtcgtc gccgatcacc tcgagcttca cccagtcggt ctcgaacgcc tcgcgtgcga
    18721 gctgcgcggt cgtcacggcg tcgcgcgccg tgtagcagcc ggccgtgttc ggcaggagcg
    18781 cgcagcccgc ctgctcgagc gcctcgacga tcgagccgcg ggccccgggg tccacgcgcc
    18841 gcagggccag ggtggcgagc tcggtgccgg acgccatcac ggcctcggcc atcgtctcga
    18901 ggttgcggaa gccgccggtg cccatgatca ggcgcgagcg gaaggtgcgt ccggcgatcg
    18961 tgaggctcat ccgccctgca ccgcctcgag cacctcgacg cgctggccgt cggcgagtgg
    19021 cgtggcctcc cagaggctgc gcggcacgac ctcgcgatcg accgccacgg cgatgccgcg
    19081 cgccagcgcc gggatgccgc tggcgctgac cgcgtcggag accttggccc cgtcgggcag
    19141 ctcccgcggc tggccgttca gcacgaggtt cacgcgtgcc tcccggcgag ggcgaagcgc
    19201 tccgggccga agccggcgag ctcctcgacc ggctcggcgc ccgtggcgag cgcggccacg
    19261 gcggacgccg tgatcggcgc cagcaggatg ccgttgcgat ggtggcccgt ggcccagatc
    19321 aggccggcgg tcgagccgcg gccgaccacc ggggcgttgt cgggcgtggc ggggcggaag
    19381 ctcgccgacg tctcgaccag ctcgcgctcc tcgacgtcgg gcagcgcctc ccaggcggcc
    19441 tcgagcaggc ggtggacgcc gcccgcggtc accgcggggt cgaagccgcg ctcctcgacg
    19501 gtggcgccga tgacgatctc gccaccggcg cgggggacga cgtagcagcg cggggtgcgg
    19561 actatgcgcg aagccagtgc gggcggaccg cccgtgggcg cggctccgcc ggacggcgcg
    19621 gctccgcctg ggccggaccc ggccccctcc cgcagccgca gcacctggcc cttcacgggc
    19681 cgcagcggcg gcgcgtcggg cgccagcgca gcgctccacg ctccggcggc caccaccacg
    19741 tgctcggcgt gcacgtgacc cgcgctcgtg acgagcgagt ggtcctccac gcccgtcacc
    19801 tccacgccct ccacgatctc gccgccttcg tgctcgaacg ccgtgcgcag agccgcaagg
    19861 gtcgcccgcg gatcggcctg gtggtcgtgc gccgcgagca cgccgccgcc gacgctcggc
    19921 gcgagcccgg gctcgaggcg gcggcattcg cgcccggtca gccactccgc gtcgagcccg
    19981 agcgagcgct ggaacgaatg caggcggcgg atctcctcca ggtcgtcgcg atcgacagcc
    20041 actaccagcg cgccgcactc gcgatagccc gtgccgaggc ccgagcgctc ggccagctcg
    20101 gccgcgaagc cgggccacgc cgccgcgccg gcgaggttga gccctagcag cgcctgctcg
    20161 ccgaactcgg cctcggtcac cggcgcgagc atcccggcgg ccaccgagct ggccccggat
    20221 ccggccgcac cgcgatcgat cgccacgacc gacagcccgc gctgggcgag ccgccacgcg
    20281 ctcgccagcc cgacgatgcc ggcccccacg acggcggcgt cgtactcgcg tgctctcggc
    20341 gcgtcggcca ctgccctctt ctcacctcct gggcgaggcg cgcaccgatc gtccctccgc
    20401 cggcattacc cggatcaggt tcaacggtcg gcggcgcacc agccgccctc tcagcccggt
    20461 cctccgagct cccgtacgag cttcatcgta gcccgccgga gcgggcgcgg cgcctagaag
    20521 cggtcgcggc gcgtgggcgc gtcgtacggg tccggccggc ggcggtcggc cagcacgaac
    20581 gtgtacagca gcgagatcac gagaccgagg atcccgacga tcatcaggat cgtgcccacg
    20641 acctggatgt cgatcgcgtc gacgtccgcg ttcacggcgt atttcaggat cgcgccgatc
    20701 gcgatcagga ctatcgaggt gccgatcgtc atgcgtggcc agaatagacg tatggcgcga
    20761 cgagaccatg cggaacggat ccggcatgcc agcctgtacc tgatcctcga ggtggcggcc
    20821 gcccacgcgg tggagccggc gctcgagggc ggtgtcgacg tggttcagat cagggacaag
    20881 cacgcccccg acggggagct cgtggccgcc ggccacgagc tcggcgacct ctgccgccgc
    20941 cacggcgcgc tgttcgtggt caacgaccgg ccggacctgg cgctcgagtg cggcgccgac
    21001 ggggtgcacg tgggacagga cgacgagtcc gtcgacgccg tgcgcgcgct cgccggcgac
    21061 cggctgttcg tgggcgtctc gacgcattcg cccgcgcagg tggccgcggc ggaggaatcg
    21121 gacgccgact acttcgcggt cggtcccgtg cacgccacgc cgacgaagcc cggccggccc
    21181 gcggtcggcc tcgagctggt gcgccacgcg gccgagcacg cccgcaagcc gtggttcgcg
    21241 atcggcggga tcgacgcggg gaacgccgcc gcggtggtcg cggcgggcgc gcgccggctc
    21301 gcggtggtcc gcgcgatccg cgacgccgac gacccgcgcg cggcggcccg cgagctgcgt
    21361 gcaatcgcgt cgggcgccac gggcgccacg ggcgccgcgg ccgccgcgag gggcggagtc
    21421 cgtgggtaag cgctcacgca agcgcccgtc gcggccgccg gccccggagc gccgcgacac
    21481 gaacgggacc cagcccgcca agaagctctc gcgctccgag gcgaaggacg cggccgcgcg
    21541 cgcggcgctc gtgccgctcg ccgagggaga gcggccgcgg gccgtgaccg tcggcgcggt
    21601 gctggcgggg cttttcgccg tcggcaacct gatcgcgtac gccgccggga tcgagatcaa
    21661 cggcgaacgg ccgcaggccg gtggggtgat cgcctactcg ctcctgatgg gagtggccgc
    21721 gtggggcatg tggaggtcga agtactgggc cgtgctcggg ctccaggcga tcctcggcat
    21781 cctgatcgtg atcttctcgc tgttcctgtt cgaggccgag aacctgaagt cgggcctgat
    21841 ctgcgtggcg gtgatcgcgc ccgccggcct cctgttcttc cacctagtca aggcgatggc
    21901 gcggatccag atgcccgacc ggcggccgtc gtcgtgagcg gcgacttctc gatcgtgcag
    21961 cgcgtgcgct tcggcgacct cgacgccatg cagcacatga acaacgtcga gtttttgcgc
    22021 ttcttcgaga ccgcccggat cgactacatc acccagctcg cacccgagca caacccgggc
    22081 aacccgaacg gcttcggctt catcttcgcc gagtgccaca tcgcctaccg cgctcccgcg
    22141 cactacaacg aggagatccg cacgttcatc cggccggtgg agctgaagcg ctcgtcgctc
    22201 aagctcgagt tcgagatgca cgtggacgcg gacgaccggc tcgtcgccga gggctggggg
    22261 acgctcgtcg gctacgacta cgagtccggc agggcgcacc cgatccccga cgcgttccgc
    22321 gagcgggtcg agccgctgct cggtcgatag cctcgggggc cgatgcccga atcgagctac
    22381 gactgcatcg tcatcggctc cggacccggc ggctacgtgg ccgccatccg cgccgcccag
    22441 ctcggcatga agacggcggt ggtcgagaag gaccgcgtcg gcgggcgctg cctcaactac
    22501 gcgtgcatcc ccgcgaaggc ggtcctgcgc gcggccgaca tcaagaccga ggtcgagcac
    22561 gggagctcga tggggatcgt cgccgagaac gtgtccgtcg acttcgacgg cgtcgtcaag
    22621 caccgcgaga aggtggtcaa gacgctgacc ggcggcgtct ccgggctctt caagaagacc
    22681 aagatcgacc tgatcgaggg cttcggcgcg ctcaccgagg acgcgaacgt gcgcatcggc
    22741 gccgccaagg acggcgacga gatcgaggcg ggcaaggtga tcctcgccac cggctcgatc
    22801 ccgaagccgc tgctggggct ccagtttggc ggccgcgtgc tgggcaccga gaccatgtgg
    22861 gcgatcg
//
LOCUS       DASVPL010000262         8637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_186879, whole genome shotgun sequence.
ACCESSION   DASVPL010000262 DASVPL010000000
VERSION     DASVPL010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186879"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..805
                     /locus_tag="VF517_14390"
     CDS             260..805
                     /locus_tag="VF517_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054177.1"
                     /translation="MTAAQHRSHRPAWRLRAAGMLALGVLLGATAPAAAQQPLPEDNA
                     GTGQYVEPVPDAGGDRPARPGSGGGGGSLPPDTRAALPSGEEGRVLQRIAGEPGSGAP
                     AGGGGAGGGSEGNGGSGGGNGASGGGARPATGEESPSAISAAASAASDPGGAGILISL
                     VAALAAAFTVVALIRRRRSDS"
     gene            complement(875..2329)
                     /locus_tag="VF517_14395"
     CDS             complement(875..2329)
                     /locus_tag="VF517_14395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX8054178.1"
                     /translation="MRPQPGAAGVETPARPPRTEVPLRDRGGFQKDALRRRLLAAADM
                     LAVVTAALLVGTLEGAASGVVVGASAPLWLVLAKTRGLYDHDHVRIRHLTLDDAPGLF
                     HWALVGTAATALFLAAMPSGSLSVAASVGLGGTVFVLAFVLRFVARTGWRHLVPPERG
                     LIVGGGPLADAFARRLSLQGGHHVEIVARVGLVDSRPPPPARRLGREVALEDLDAIVV
                     DEEIERVVVALQDLSEETLAHIASTCRSLGVKLSVAPPMRAMLGTAVHLGRLLELPLI
                     EFRTWDPSRSTMLMKRAVDVVGASVALALAAPLMLAIAVAVRLDSPGPALFRQRRAGR
                     GGVPFQMLKFRTMTADAEARIHEVVAIDELTEPVFKLRSDPRITRVGRVLRRTSLDEL
                     PQLINVLRGDMSLVGPRPEEVWLVERYEEADRFRLEMRPGLTGPMQVQGRGELTFQER
                     TAVEREYVENYSLRKDVKILLQTFSAVVGGRGAY"
     gene            complement(2405..4333)
                     /locus_tag="VF517_14400"
     CDS             complement(2405..4333)
                     /locus_tag="VF517_14400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX8054179.1"
                     /translation="MEITFVQAPRQNAFFGEIVEAIRDELRRAGVDSSVSHSGFPPPR
                     RGMVYALVPPHEWFALHARRHPPTTGQLQRTVGICAEQPGTSFFEDDVTLAASIGAVM
                     DINAAGVRELGRRGVRATHFPLGWTPSWSHWSPDASEGDGHEPARDVDVLHLGIHSDR
                     RAAILAESARHLARWRCHLNLGDDHGPNFRPQANYVTGAAKWELLRRSRTLLNIHVGD
                     RPYFEWTRVVQAICNGCVVVSEHSSHFEPLVAGHHFVSARPEALALLAQPILEVESRR
                     ASMARDAWHLLKEQLPLRDSVARLIEAAHDLDRHPVFTGVTVSIPLQSPAAPLDSEDD
                     DRDEFERATSYPHLPGDHETSVVRHALKATQLEVIGLRRELARSRLEALDGETATSIR
                     VAHESPAYRAARPRVSVLVSLFNYDRHVRAALDSCAASSLTDIELVVVDDGSSDRSRQ
                     VVREWSEAKPAVPLLLLAHPCNRGLSAARNSALDFARAELAFILDADNCLYPAGLERL
                     VAALDQDPEVAFAYGMLEVLNAGRSVGLRSFYPWRPARLRTGNYIDAMALWRTRSLRE
                     LGGYTDDLRLYGWEDYDLWCRAAEREVEGVLVPEIVARYRSTGHSMLAVTNISWKAAV
                     SLLVDRHPKLMANVQPPP"
     gene            4360..5406
                     /locus_tag="VF517_14405"
     CDS             4360..5406
                     /locus_tag="VF517_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8054180.1"
                     /translation="MSNDVSIAGSDLTNKVDGVPERFDPASMHGELAEAEHLVRYSWA
                     ARLAGGRRVLDAGCGLGYGSRMLHRAGASEVVGVDIGAGVIEAAAAGDEPGLRFEVGD
                     LNGLDFEDGRFDLVVCLEVIEHVHDQRRVIAELSRLLSPEGILVVSSPNRAVYVPGNP
                     HHVHEYLPDELHAALAESLEHVRLWRQHGWICSAILDEEAFASSDVTPLPDLWVEKAV
                     GEEPGRETYTLALGSNAPLPEPAPGAVITGAVEVRKWLDLYHEQDQVLRGLHDHFARL
                     GSRDGELNDLRLQLEQAERALAQLPLLERELEELRARAEAGERNLANVLSSPSWRVTT
                     PLRAVKNLLRRPRS"
     gene            complement(5407..6822)
                     /locus_tag="VF517_14410"
     CDS             complement(5407..6822)
                     /locus_tag="VF517_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054181.1"
                     /translation="MRLLAITLVMAAVAGLPAVAHGAGRFDVGLTDPLDGAFGEKDPP
                     GAYDAARDAGVRFVRVNVQWHLVAANRPANPTSPDDPAYGWSWLDERVTGLKSRGIEP
                     VLSLYSAPEWARSSKGGATRVTPNVGDLENFATAVARRYGGSTPGVPRVRYWQIWNEP
                     NLKTYLDQSDSVEQYRAMVTASYAAIHGVHDDNRVVAGGLAPYAAYAGDHAPLRFMRE
                     LLCMSAGSKPRPTCGARVPFDAWSVHPYTSGGPNRSARARDDVSLGDLPEVHRLLLAA
                     ERAGHVKPKGSLDFWVTEFSWDTKGPDPWGVPLALHARWVAEAFYNMWRNGVSMAVWF
                     QLRDNPQGTFSWGQTFQSGLFFRTADRYADERAKPVRDVVRFPFVALPARGGAAVWGR
                     IPTGSKGTVVVERRAGKRWVRVANLGAGGSGIFRRTLRVAARTVLRARYGSDASVPFK
                     VERSPDRNVNPFGGPPGNRLS"
     gene            complement(6822..7856)
                     /locus_tag="VF517_14415"
     CDS             complement(6822..7856)
                     /locus_tag="VF517_14415"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEX8054182.1"
                     /translation="MRQRSFILLAVTLAVMIFGSVGVYAYDSSNDEIAEGITAAGIDI
                     GGMTESAAREKLRREVAYDLERPLKVHYKGRSFTLKPERARIRTNVEEMVEQAVEHSR
                     EGNVISRSLRSITGGEIHKDIPVKVTFDKRAVTALVKRVKRKVDLPARDASVDFAGGS
                     IQPVAGKNGRRLDAGRLTSQIESALVEPTSERVVRARVVVTKPEVTTKELASKYPKIL
                     VVSKATTSVTLYENLKPTKTYQVAVGSPGYPTPNGRFAIQNKQVDPVWQVPNSDWAGD
                     LAGRTIPPGPQNPLKARWMGIYGGAGFHGTSDVGSIGSAASHGCVRMAVPDVIDLFDR
                     VDVGTPVFVS"
     gene            complement(7951..>8637)
                     /locus_tag="VF517_14420"
     CDS             complement(7951..>8637)
                     /locus_tag="VF517_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054183.1"
                     /translation="APVLPPWATTLALGPMRRLGVPVPPEMLGQLRFGRGLDNRKLKA
                     SGFSYRYTSRETALAFAEHTRLASVMRGTAEPYRYEREVEDFLRWSPNVRNPSVNREH
                     RLTPAELLELRRLHAGYAARTGAPGTDSPSRQAQSLAHTARAMEGDDAPANGGAAATA
                     ETAGSTTAPPTSIEHYDDLTADEIVPLVESLESPDLLALRDHERAGQGRPRVLAAIDG
                     VLVRRESRQP"
BASE COUNT         1186 a         3102 c         3203 g         1146 t
ORIGIN      
        1 gtcatcgaga gcgccgtgag gagcgtgaag gcgagcagcc ccccgccggc cacgagggcg
       61 gcgcgcggca cccgcgcccg cggccagagc ccgagcccca ggcccacgat caccgcgacc
      121 cacaccacga gcgcggctcc cgcgcggaac ccggacgagt agccaccgcc cgcggccgcc
      181 gtcgcgatac acgcgagcgc gactaatgcc gctgtaacgt gttggattcg acgcacgatg
      241 accgggaacc gctaaccgaa tgaccgccgc acagcatcgc tcccaccgcc ccgcgtggcg
      301 gctgcgggcc gccgggatgc tagccctcgg cgtactcctg ggcgcgaccg cgccggccgc
      361 ggcccagcag ccgctccccg aggacaacgc cgggaccggc cagtacgtcg agcccgtgcc
      421 ggatgccggc ggcgaccgcc cggcgcggcc tggctcgggc ggtggcgggg gctccctccc
      481 gccggacacg cgggccgcgc tgccctccgg cgaggagggc cgcgtgctcc agcggatcgc
      541 gggcgagccc ggttccggcg ccccggcggg cggcggcggc gcgggcggag gcagcgaggg
      601 caacggcggc agcggcggcg gcaacggcgc gagcggcggc ggggcccggc cggcgaccgg
      661 cgaggagtcg ccgagcgcga tctccgcggc ggcgagcgcg gcgtccgatc cgggcggtgc
      721 cgggatcttg atctcgctgg tggccgcact ggcagcggcg ttcaccgtcg tggccctgat
      781 ccgccggcgg cgctccgact cctagcggag agcgagtccg cgggcggctt cggcgaggtg
      841 cgcttcgcgg gcgcgagcgc gggtgtcggc tgggttagta ggccccgcgg ccgccgacga
      901 ccgcggagaa cgtctggagc aggatcttca cgtccttgcg gagcgagtag ttctcgacgt
      961 actcgcgctc gaccgcggtc cgctcctgga aggtcagctc gccgcgcccc tggacctgca
     1021 tcggtccggt gagcccgggc cgcatctcca gccggaagcg gtcggcctcc tcgtagcgct
     1081 ccacgagcca gacctcctcg gggcgcggcc cgacgaggct catgtcgccg cgcaggacgt
     1141 tgatgagctg cggcagctcg tcgaggctgg tgcgtcgcag gacgcggccg acgcgggtga
     1201 tgcgcgggtc ggagcggagc ttgaagaccg gctcggtgag ctcgtcgatc gcgaccacct
     1261 cgtggatgcg cgcctcggcg tcggcggtca tggtcctgaa cttgagcatc tgaaacggaa
     1321 cgccaccacg acctgcgcgg cgctggcgga acagggccgg gccgggcgag tcgagcctca
     1381 cggccacggc gatcgccagc attagcggtg cggcgagggc gagcgccacg ctggccccga
     1441 cgacgtccac ggcgcgcttc atcagcatgg tcgagcgcga tgggtcccac gtgcggaact
     1501 caatcagcgg cagctcgaga agccggccga ggtggacggc ggtgcccagc atcgcgcgca
     1561 tcggcggggc gacgctcagc ttcacgccga gcgaccggca ggtggaggcg atgtgggcga
     1621 gcgtctcctc gctcaggtcc tggagcgcga ccacgacccg ctcgatctcc tcgtccacga
     1681 cgatcgcgtc gaggtcctcc agggccacct cacgcccgag ccggcgggcc ggcggcggcg
     1741 ggcgcgagtc gaccagcccg acgcgcgcca cgatctccac gtggtggccg ccctggagcg
     1801 agagccgccg cgcgaacgca tccgcgagcg gcccgccgcc cacgatcagc ccgcgctcgg
     1861 gcggcaccag gtgccgccat cccgtccgcg ccacgaagcg cagcacgaac gccagcacga
     1921 acaccgtgcc cccgagcccc accgaggccg ccaccgacag ggagccgctc ggcatcgcgg
     1981 ccaggaagag cgcggtcgcg gccgtgccca ccagggccca gtggaacagg cctggggcat
     2041 cgtcgagggt caggtggcgg atccggacgt gatcgtggtc gtagagcccg cgcgtcttgg
     2101 cgagcaccag ccagagcggc gcgctggcgc ccaccacgac cccggacgcc gcaccctcca
     2161 gggtccccac cagcagggcc gcggtcacga ccgcgagcat gtcggcggcg gccagaagcc
     2221 ggcgcctcag agcgtccttc tggaacccgc cgcggtcgcg cagcgggacc tccgtgcgcg
     2281 gcggacgagc cggcgtctcg acgccggccg cgccaggctg cggcctcacg tcctccactc
     2341 cccgcgtcac gaccctcgcc gtccccgccc tagcactgtg cccgagtgta aacccacatg
     2401 gcgctcacgg cggcggttgg acgttcgcca tcagcttcgg gtggcggtcg acgagcagcg
     2461 agaccgccgc cttccacgag atgttcgtga ccgcgagcat cgagtgcccg gtggagcggt
     2521 agcgggccac gatctcgggc acgagcacgc cttcgacctc gcgctccgcg gcgcggcacc
     2581 agaggtcgta gtcctcccag ccatacaggc ggaggtcgtc ggtgtagccg ccgagctcac
     2641 gcagagagcg cgtgcgccac agggccatcg cgtcgatgta gttgccggtg cgaagccgcg
     2701 cggggcgcca cgggtagaag ctcctgaggc ccaccgaccg cccggcgttc agcacctcga
     2761 gcatgccgta ggcgaacgcg acctcggggt cctggtcgag cgccgcgacg agccgctcga
     2821 gcccagccgg gtagaggcag ttgtccgcgt cgaggatgaa cgccagctcg gcgcgggcga
     2881 agtcgagcgc cgagttgcgc gcggcgctca ggccgcggtt gcatgggtga gcgagcagca
     2941 gcaggggcac cgcgggcttt gcctcgctcc actcgcgcac cacctggcgc gagcggtccg
     3001 aggaaccgtc gtccacgacc acgagctcga tgtccgtgag gctgcttgcc gcacacgagt
     3061 cgagcgcggc gcgaacgtgc cggtcgtagt tgaacaggct caccagcacc gagacgcgcg
     3121 gcctggcggc cctgtaggcg ggtgactcgt gcgcgacgcg gatgctggtc gcggtctcgc
     3181 cgtcgagcgc ctcgagccgc gagcgggcca gctcgcggcg caggccgatg acctcgagct
     3241 gcgtcgcctt cagcgcgtgg cgcacgaccg acgtctcgtg gtcgcccggc aggtgcgggt
     3301 agctggtggc ccgctcgaac tcgtcgcggt cgtcgtcctc ggagtcgagc ggggccgccg
     3361 gcgactggag cgggatcgac accgtgacgc cggtgaagac ggggtggcga tcgagatcgt
     3421 gggccgcctc gatcaggcgt gcgaccgagt cccgcagcgg cagctgctcc ttgagcaggt
     3481 gccacgcgtc gcgggccatc gacgcacgcc gcgactcgac ctcgaggatc ggctgagcga
     3541 gcagcgccag cgcctcgggc cgcgccgaca cgaagtggtg accggcgacc aacggctcga
     3601 agtgggagga gtgctcgctg acgacgacgc agccgttgca gatcgcctgg accacgcggg
     3661 tccactcgaa gtacgggcga tccccgacgt ggatgttgag cagtgtccgc gagcggcgca
     3721 gcagctccca cttcgccgcg ccggtgacgt agttcgcctg cggccggaag ttcggcccgt
     3781 ggtcgtcgcc gagattcaga tggcagcgcc agcgagcgag gtggcgggcc gactcggcga
     3841 ggatcgccgc gcggcggtcg ctgtggatgc ccaggtggag cacgtccacg tcgcgcgcgg
     3901 gctcgtgtcc gtcgccctcg gacgcgtcgg gcgaccagtg gctccacgac ggggtccagc
     3961 ccagcgggaa gtgggttgct cgaacgccgc gccgcccgag ctcgcgtacg ccggccgcgt
     4021 tgatgtccat caccgctccg atcgacgcgg cgagcgtgac gtcgtcctcg aagaacgagg
     4081 tgcccggctg ctccgcgcag atcccgaccg tccgctggag ctggccggtc gtcggcggat
     4141 ggcgccgcgc gtgcagcgcg aaccactcgt gcggcggcac gagcgcgtac accatgccgc
     4201 gtcgcggcgg cgggaagccg ctgtggctga ccgaggaatc gacaccggca cggcgcagct
     4261 cgtcgcggat cgcctctacg atctcgccga agaacgcgtt ctggcgcggg gcttgtacga
     4321 aggtgatctc cactgggcgc ggatactagg tctgcaaagt tgagcaacga cgtgtcgata
     4381 gccggctctg atctcaccaa caaggtcgac ggggtcccgg agcggttcga ccctgcctcg
     4441 atgcacggcg agctcgccga ggccgagcac ctcgtcaggt attcctgggc ggcgcgcctg
     4501 gccggcgggc ggcgcgtgct cgacgccggc tgcggactcg ggtacggctc gcggatgctg
     4561 caccgagcgg gtgcctccga agtcgtcgga gtggacatcg gcgcgggcgt gatcgaggcg
     4621 gcggcggccg gcgacgagcc cgggctgcgc ttcgaggtgg gcgacctcaa cggcctcgac
     4681 ttcgaggacg gccgcttcga cctcgtcgtg tgcctcgagg tcatcgagca cgtccacgac
     4741 cagcgccgtg tgatcgccga gctcagccgg ctcctctccc ccgaggggat cctcgtggtg
     4801 tcctccccca accgggccgt gtacgtgccc ggcaacccgc atcacgtcca cgagtacctg
     4861 ccggacgagc tgcacgccgc cctcgccgag tcgctcgagc acgtgcgcct gtggcgccag
     4921 cacggctgga tctgctccgc gatcctcgac gaggaggcgt tcgcaagcag tgacgtgacc
     4981 ccgctgcccg acctctgggt cgagaaggcc gtgggcgagg agccgggccg cgagacctac
     5041 acgctcgcgc tcggcagcaa cgcgccgctt cccgagcccg cgcccggcgc ggtgatcacc
     5101 ggtgccgtgg aggtccgcaa gtggctcgac ctctaccacg agcaggacca ggtcctgcgc
     5161 gggctgcacg accacttcgc gcggctcggc agccgcgacg gcgagctgaa cgacctccgc
     5221 ctgcagctcg agcaggccga gcgggcgctc gctcagctgc cgctgctcga gcgggagctc
     5281 gaggagctgc gggcgcgcgc cgaggccggg gagcgcaacc tcgcgaacgt gctgtcgtcg
     5341 ccgagctggc gtgtgaccac gccgctgcgc gcggtcaaga acctgctcag gcgcccgcgg
     5401 agctgatcag gacagccggt tgcccggcgg gccgccgaac gggttcacgt tgcggtcggg
     5461 ggagcgctcg accttgaagg gcaccgacgc gtcgctgccg tagcgggcgc ggaggacggt
     5521 ccgggccgcc acgcggagcg tccgccggaa gatgccgctg ccgccggccc ccaggttcgc
     5581 gacgcggacc cagcgcttgc cggcgcgccg ctccacgacg acggtcccct tcgacccggt
     5641 cgggatcctg ccccacaccg cggcgccgcc gcgagccggc agcgcgacga atgggaaccg
     5701 gaccacgtcg cgcacgggct tggcgcgctc gtcggcgtag cggtcggccg tccggaagaa
     5761 caggccggac tggaacgtct ggccccagct gaaggtgccc tgcgggttgt cgcggagctg
     5821 gaaccagacg gccatgctca ccccgttgcg ccacatgttg tagaaggcct cggcgaccca
     5881 gcgcgcgtgc agcgcgagcg ggacgcccca cggatccggc cccttcgtgt cccacgagaa
     5941 ctccgtcacc cagaagtcga gcgacccctt cggcttcacg tgtcccgcgc gctcggcggc
     6001 gagcagcagg cgatgcacct cgggcaggtc gccgagcgag acgtcgtcgc gggcccgggc
     6061 gctgcggttg gggccgccgc tcgtgtaggg gtggaccgac cacgcgtcga acggcacgcg
     6121 cgcgccgcag gtcgggcgcg gcttcgagcc ggcgctcatg cagagcagct cgcgcatgaa
     6181 cctgagcggc gcgtggtcgc cggcgtaggc ggcgtacggc gccagccccc cggccacgac
     6241 gcggttgtcg tcgtgcaccc cgtggatcgc ggcgtacgag gccgtgacca tggcccggta
     6301 ctgctcgacc gagtcgctct ggtccaggta cgtcttcagg ttcggctcgt tccagatctg
     6361 ccagtacctg acccgcggga cgccgggcgt cgagccgccg tagcggcgcg cgaccgcggt
     6421 cgcgaagttc tccaggtcac cgacgttcgg cgtcacgcgc gtcgcgccgc ccttgctgga
     6481 ccgcgcccat tcgggcgccg agtagagcga gagcaccggt tcgataccgc ggctcttgag
     6541 ccccgtgacc cgctcgtcga gccagctcca gccgtacgcc ggatcgtcgg gcgaggtcgg
     6601 gttggccggc cggttggccg ccacgaggtg ccactgcacg ttcacgcgca cgaagcgcac
     6661 gcccgcgtcg cgggcggcgt cgtaggcgcc gggcgggtcc ttctccccga aggccccgtc
     6721 gagggggtcg gtgagcccga cgtcgaaccg ccccgccccg tgggcgaccg cgggcaggcc
     6781 ggcgacggcg gccatcacca gcgtgatcgc gaggagacgc actagctcac gaacacgggc
     6841 gtaccgacat cgacgcggtc gaacaggtcg atcacgtcgg ggaccgccat ccgtacgcag
     6901 ccgtgcgacg cggcgctgcc gatcgagccc acgtccgagg tgccgtggaa gccggccccg
     6961 ccgtagatcc ccatccagcg cgccttcagc gggttctgcg gccccggcgg gatcgtgcgg
     7021 ccggcgaggt cgcccgccca gtcgctgttg gggacctgcc agaccgggtc gacctgcttg
     7081 ttctggatcg cgaagcgccc gttcggcgtc gggtagccgg gcgagccgac ggccacctgg
     7141 taagtcttcg tcggcttgag gttctcgtag agcgtgaccg aagtggtggc cttgctgacc
     7201 acgaggatct tcgggtactt cgacgcgagc tccttggtgg tgacctcggg cttggtgacg
     7261 accacccgcg cgcgcaccac gcgctcggag gtcggctcga cgagcgccga ctcgatctgg
     7321 ctcgtgagcc ggcccgcgtc gaggcggcgg ccgttcttgc ccgccaccgg ctggatgctg
     7381 ccgccggcga agtcgaccga ggcgtcgcgc gccggcaggt cgaccttgcg cttcacgcgc
     7441 ttgaccaggg cggtcacggc gcgcttgtcg aaggtcacct tcacggggat gtccttgtgg
     7501 atctccccac cggtgatcga gcgcaggctg cggctgatca cgttgccctc gcggctgtgc
     7561 tcgacggcct gctcgaccat ctcctcgacg ttcgtcctga tccgcgcgcg ctcgggcttg
     7621 agcgtgaacg agcggccctt gtagtgcacc ttcagcgggc gctcgaggtc gtaggcgacc
     7681 tcgcggcgaa gcttctcgcg ggcggcgctc tccgtcatgc cgccgatgtc gattcccgcg
     7741 gcggtgatgc cctccgcgat ctcgtcgttg ctcgagtcgt aggcgtagac gcccaccgag
     7801 ccgaagatca tcacggccag ggtgaccgcc aggagaatga acgagcgctg ccgcataccg
     7861 aggtccggcc agtctaggcc gaggggcgga tttttcccct aattgcgggg aaaccgcgcc
     7921 aactggaaga gttcttgcac gccgtccggt ctatggctgg cgcgactcgc ggcgcacgag
     7981 caccccgtcg atcgccgcga gcacccgcgg gcggccctgc ccggcccgct cgtggtcccg
     8041 cagggccagc agatcggggc tctcgagcga ctccaccagc ggcacgatct cgtcggcggt
     8101 gaggtcgtcg tagtgctcga tcgacgtcgg cggggccgtg gtcgagccgg ccgtctcggc
     8161 cgtggcggcc gcgccgccgt tggcgggcgc gtcgtcgccc tccatcgcgc gcgcggtgtg
     8221 cgcgagcgac tgcgcttggc gcgacggcga gtcggtgccg ggggcgccgg tgcgcgcggc
     8281 gtagccggcg tggagacggc gcagctcgag cagctcggcg ggggtgagcc ggtgctcgcg
     8341 gttcacggac gggttgcgca cgttcgggct ccagcgcagg aagtcctcca cctcgcgctc
     8401 gtagcggtac ggctcggcgg tcccgcgcat gaccgacgcg agccgcgtgt gctcggcgaa
     8461 ggcgagcgcc gtctcgcgcg aggtgtagcg gtagctgaag ccgctggcct tgagcttgcg
     8521 gttgtcgagg ccgcggccga agcgcagctg gcccagcatc tcgggcggca ccgggacgcc
     8581 caggcggcgc atcgggccga gcgcgagcgt cgtcgcccac ggcggcagga ccggcgc
//
LOCUS       DASVPL010000263        19899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_186938, whole genome shotgun sequence.
ACCESSION   DASVPL010000263 DASVPL010000000
VERSION     DASVPL010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 19899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19899
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186938"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF517_14425"
     CDS             complement(<1..401)
                     /locus_tag="VF517_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054184.1"
                     /translation="MGGIARTAACRFSIVVGLALAGLVAGPGVARAGTYEVVACDTAP
                     GGAHGSWSPRASEKMGTMQWCPSAGREAAGLWAGNGLNVGTIPAFAASQQYFDAPAGT
                     SIVFFAARYMFRRFDPYWRLGVFADTQMLHG"
     gene            complement(608..1105)
                     /locus_tag="VF517_14430"
     CDS             complement(608..1105)
                     /locus_tag="VF517_14430"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEX8054185.1"
                     /translation="MAGEPTSMLGAHARNGGDVHGSPPTDASDYAVINLVYGALFTGV
                     ILATRERARKDPVQLHELIPMSAATFALAKVIAKEKVGTWVREPFVEHVEGEAKPEGR
                     GLRRAVGELVTCTRCVGAWSALGVVGLRLAYPDAGRNVTNVLAVSGANDWLQAGFKAV
                     CARSD"
     gene            1147..2196
                     /locus_tag="VF517_14435"
     CDS             1147..2196
                     /locus_tag="VF517_14435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX8054186.1"
                     /translation="MVDWSLARQIARFASGAGGSAEVDADFPAMVADAHGHLRSYTGL
                     EPVQPIPPPEVVDRAEWADVNLTSLCRMLEPVSEGLSSRLENAGPFAGPLRAVTGATL
                     AAESGLVMGYMSQRVLGQYELSLLEAEASPRLLFVGPNLNKAVRELGADRDSFLRWIV
                     LHELTHVLQFQGVPWLREHLSSLMREYMRTVEVRMERGAAGGLPSWPDPQRIVEAFRE
                     GGLVALVQTREQRKIMEKVQPAMSVIEGYSEHVMDAVGERVLSGYAGLRDGMERRRRS
                     RSAPERILQRLLGLDMKMRQYEEGKAFCDGVVERGGIEALNRVWDSPQALPTQAELKD
                     PAGWVTRVQALPAAA"
     gene            2341..3075
                     /locus_tag="VF517_14440"
     CDS             2341..3075
                     /locus_tag="VF517_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054187.1"
                     /translation="MAPWTRLSPLALVLALVCLGVTAASASAANSNRLYVNASADRPA
                     GVDASRLYDVADHSASRWKLSVEGNASRMPGVKDGTQVIGFSKATAAQALGVTTVWSR
                     TLYKVVTTRRCWRQNGRRRCQTTRRYVKNGVEVVEKDVQLNPSVPWEQGPSYPSPTRF
                     DLESTILHELGHFANPLKDNHVFGCENSPMIDSIAPGEFWRDADDWLRYGCSASAGGR
                     SKLAVPAAPQMRMLVVEHRLPPRFEH"
     gene            3375..4022
                     /locus_tag="VF517_14445"
     CDS             3375..4022
                     /locus_tag="VF517_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054188.1"
                     /translation="MAQQSTSSNRRSTASKRSTSSRSTSSANGNSKRTTTGTQRSARA
                     KSAAASRQRNTANRQAARTRSNARRTTTQAQTTARQAAGAAAQRVKAETTAVQAVVQT
                     AQRGVLIPVGAALTARDNVVETVRPYTRGRTSIEREVTRIQKRVSTNLRRFERRGETA
                     RTRLQRQVKRTRTQVEREARQRRNQVQRSVKTAGKDFGRGFDRIQAGVDELNPVS"
     gene            4182..5336
                     /locus_tag="VF517_14450"
     CDS             4182..5336
                     /locus_tag="VF517_14450"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX8054189.1"
                     /translation="MPSEDQIRDALRVVIDPELRQNIVDLGMVRSIRQPVHGRIEVVV
                     SLTTPGCPIRSHFQTAVVDAVSALEGVAQVEVGFDVLSADEKQGLQQRLGRQGGLPSG
                     ALARVKNVICVGSGKGGVGKSTITANLAAALTAEGHPAAAMDADVWGYSIPRMLGVHG
                     KPRVNERRKIVPLVGAGGVQAISIEFFLERQDQAIVWRGPMLHKAIRQFLEDVEWGEL
                     DYLLIDLPPGTGDVSMTLAQLLPQARFVIVTTPQPAAQNVAKRAAETAIRFDLEIAGV
                     VENMSAFNGPDGERVTIFGEGGGQVLADELEVPLLGKVPLAEDLRVGADEGEPLVLTD
                     PDAPAAQAIFHAARGLVAATPQESPLLQVANGGLEPAAAPVTGTPLPMAP"
     gene            5495..7084
                     /locus_tag="VF517_14455"
     CDS             5495..7084
                     /locus_tag="VF517_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054190.1"
                     /translation="MTSVGRMRREDGFSVVELVIAMAVILVGVLGTLVLLDRANAQTS
                     SAKTRQTANALVRDIVETAQGTGYTQLTPTSVKSTLQSNGFPDDIPASSSTWEIKRNG
                     IVFSVAATACIVDDPGDGLATHAADAGYCSDSPGGTGDNSGDDYRRVTLTVTPPTSIG
                     TPVTQTTVVGANRITNPGGSGPGGGTATSNDVKEMKIISPTLYSGQVAPCQNNATCTF
                     PAATTTAVTPKSVSFRAVTAYTAQRVVFTVDGLVTATLTGPATTFDWTWNLPDSQPDG
                     NYVVAAQIFDANGVNAISSPSPLVVTVNRYVPDQNAFAPTAAGRNPLWSNLPEMETYP
                     TADTSARVDRDITGFLATRFLNGAANGTACQTFSPNARGCLDTSPPSCCSSTVTYRIA
                     PTGANPDGSQQLGPNTAASPNVNAVNTRPVAPDNVTIVRTGNRVTITWVNPSGSGDPD
                     PGDCIDFYRVYRRDLNGTTWEYTDRADRTTFGNAVAPCGGSGESSNTITLTEDTNTAK
                     RYRVTAVDTHLAESPKENISG"
     gene            7081..8040
                     /locus_tag="VF517_14460"
     CDS             7081..8040
                     /locus_tag="VF517_14460"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8054191.1"
                     /translation="MIARAIRNRLRSQRGFTLVEVLVAGSLSIIVIGAALAPFEVLNR
                     TGRVTANQNDSQDNARNAVVMITRNLRNTSGQNQLVNLASPYDLVIETVDPTPKPSGS
                     QNARNLMRVRYCLDTTSGGSVSLTSGRIWEQSLRWTTATPPTTLPSVNCPDNSWGTKR
                     AVANFITNRATSTTRPTAVPLFSYFPTGASLDMITSIRVSIYSDRDWTHAPRETELTS
                     GVLLRNQNGSPTASFTAIPGVAGSRKITLNAAASTDPEGLPLTYRWCDVTTVSTCDDT
                     TRIGTSTLYTYTAPAAGNRKIVLQAFDIGGLQSTAGPVTVVAP"
     gene            8037..9248
                     /locus_tag="VF517_14465"
     CDS             8037..9248
                     /locus_tag="VF517_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054192.1"
                     /translation="MRTRIVHALRARAADESGLALVLAVMLTMLMLASAMALLAFTDN
                     QTKMTGKERLRESTLTLAEGALNAQANLLSAGWPETSDKAFVPCTNKSTTVRCPDAGN
                     LLRGFASKDFGNSAPISWNLSVRDNGLGSFYDDVATASQPAWDQSGPGGGAPDGVMWL
                     RAQATIRGESRTIVSLIRATPIARAFPRGVLTAGHFHTLNNGNKQLLDTGSGPGVLAR
                     CTVGPGGPDRGNPCLDYQPTKGQVYPNSWVSDPSIPSAMSPNEIDLLRNRAKAANTWF
                     STCPSTVPTAPLVFVEDGSCQINSGSWVNSPAAPGMLVMYRGDLIIQGSAGFYGLIYA
                     VNADNRADNLVTVTGGAYVSGAIVVDGRGGLAIDGNGLVLQYDSNAFNLVTTTQTINV
                     IANSWRELNGH"
     gene            complement(9273..9539)
                     /locus_tag="VF517_14470"
     CDS             complement(9273..9539)
                     /locus_tag="VF517_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX8054193.1"
                     /translation="MAYVIAEPCIDTKDNSCVEVCPVDCIHPTPDEPDYDDVKMLYID
                     PDECIDCDACVEACPVDACFAEDQLPDEWQNYIQVNADYYKSKQ"
     gene            9622..10500
                     /locus_tag="VF517_14475"
     CDS             9622..10500
                     /locus_tag="VF517_14475"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8054194.1"
                     /translation="MILLTGATGNLGSALLARLVADRQRVRCLVRDPRGLGPDRVRVQ
                     IALGDLADPASFRNALRGVKTVVHLAASIRDQPRASIEELNAVATLRLLRAAEKAGVE
                     RFVFFSALGASLHERARFFRAKALAEQAVEDAEIPSVVFAPSIVYSPRDPWVRLLERM
                     SLLPVMPISGSGQARYQPIWADDAAACVMAALGADGAAHTRYELAGPQTLSYDDIALE
                     TLGALRRRRRLVHVPLPVVRRGLRAVEAVVGAQAFAVWEEAELMEIPMTTPRGTADAE
                     ALGVSPRPMRAVLGAR"
     gene            complement(10460..11335)
                     /locus_tag="VF517_14480"
     CDS             complement(10460..11335)
                     /locus_tag="VF517_14480"
                     /inference="COORDINATES: protein motif:HMM:NF021577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HEX8054195.1"
                     /translation="MANDLTIREVAARTGVNAATLRMWEQRYGFPEPGRLDSGHRRYS
                     DEDVERVRRVARDRAAGLSIPAAIDRALNATAEVEPSIYAGVRRRRPDLIPYLLPKRT
                     LIALSHAIEDECVARAERPVLFGSFQRPSFFRHAEARWRELTRTAHAAIVFAEFDERR
                     DPDGGPHEVPIEHEHPLCREWALVCDAPDYSAFLAAWERPGQDAIRDYERRFETVWSV
                     EPELVREAARVAAGIAEQGAPDIVSAIRERLQDTPPPSADEFRLVGALTNRMVAYVGS
                     ETANGLPAPHASVAD"
     gene            complement(11341..11871)
                     /locus_tag="VF517_14485"
     CDS             complement(11341..11871)
                     /locus_tag="VF517_14485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8054196.1"
                     /translation="MSPNLKDPRQFDDAYRQLAPQAVAAANRVLRDEAAAEDVVQDVF
                     MQLWLRPSAYDAARGPLSAYVTMMARSRALDRWRTRSARDNAVERTKEEVRVTGRGAE
                     ESADEQVIRRERSRTLAGALRHLPAEQRDALLLAYGRGMTASEIAEVSAIPLGTAKSR
                     LRLGLRKTRENLAAAA"
     gene            complement(12130..13764)
                     /gene="groL"
                     /locus_tag="VF517_14490"
     CDS             complement(12130..13764)
                     /gene="groL"
                     /locus_tag="VF517_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX8054197.1"
                     /translation="MAHKELKYDQDARSALEAGVDAVANAVKVTLGPKGRYVVLDKKF
                     GAPTITNDGVTIAREIEVEDVFENQGAQLVREVATATNDVAGDGTTTATVLAQQIVRT
                     GLKNVAAGANPLGLKRGIEKAVDQVVDEIRKQAKEISGKDQIARVAAISAADDEIGDI
                     IADAIEKVGKDGVVNVEEGQTFGMDLEFTEGMQFDKGYISPYMVTDQDRMEAVLEDPY
                     ILIANQKIGSVRDLLPVLEQVIQAGKSLLIIAEDVEGEALATLVVNKLRGTFSAVAVK
                     APGFGDRRKRMLEDIAILSGGEVITEEMGLKLENTQISQLGRARRVVVAKDNTTVIDG
                     AGEAEAIKGRINQIKNEIENTDSDFDREKLQERLAKLAGGVAVVKVGAATETEMKEKK
                     HRVEDALQATRAALEEGIVPGGGVALLRAANAVKVDAIDDADEQTGARIILRALEEPV
                     RQLANNAGLEGSVVVNDVRKAKAGHGLNADTGEIVNLVEAGVIDPAMVTRSALQNAAS
                     IGKNILTTEAIVAEAPEKDGAGGMGGGMPGGMDGMM"
     gene            complement(13800..14090)
                     /gene="groES"
                     /locus_tag="VF517_14495"
     CDS             complement(13800..14090)
                     /gene="groES"
                     /locus_tag="VF517_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX8054198.1"
                     /translation="MKLKPLGDRLIVKAVEEEETTASGIVLPDTAKEKPQRGKVLAVG
                     DGKLSEDGGNRIPLDVAEGDEVLYSKYGGTEINIEGEDLLVLRESDVLAKVA"
     gene            complement(14204..14527)
                     /locus_tag="VF517_14500"
     CDS             complement(14204..14527)
                     /locus_tag="VF517_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054199.1"
                     /translation="MARSERDRKQTSRVLGVLFAALPFALVQGVFASLLGDGVKRRAC
                     DVEDSGGYLVLLAAVAFGAVVLAVLLALPRPRWAAGVLGFAGLASLVFAAAGGWGASN
                     CALGI"
     gene            complement(14543..14941)
                     /locus_tag="VF517_14505"
     CDS             complement(14543..14941)
                     /locus_tag="VF517_14505"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX8054200.1"
                     /translation="MFVLNFYSPVFVDQLKRGRKTATIRLGDKSRKYQKGEVVLITVG
                     FQHSPREKIFEAVIDAVDVKKVSELSPRDIEHDNPEFRRVEETAHFLEQIYGRPVSTD
                     DTVTVVRFSAIYDRPEEITERMRPAAPAQN"
     gene            complement(14955..15374)
                     /locus_tag="VF517_14510"
     CDS             complement(14955..15374)
                     /locus_tag="VF517_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8054201.1"
                     /translation="MIREFSAGGVCVRRMRGRDFVAAVRVKGGTVLALPKGHIDPGES
                     SAEAAAREVREEAGVTGDLVEKLGDVRYWYRRDGDRVMKNVAFFLFRYSSGSVADHDH
                     EVDSAEWVPLEDAPRLLAYRGERDMASAALSALGAAR"
     gene            complement(15371..16024)
                     /locus_tag="VF517_14515"
     CDS             complement(15371..16024)
                     /locus_tag="VF517_14515"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX8054202.1"
                     /translation="MSLRRSATTSTEDARARMVEGQLRERGIADERVLAAMAELPREA
                     FLPEEERDRAYRDGAVPIGAGQTMSQPWIVARMTALLDLTGEELVLEVGTGSGYGAAV
                     LSRCCRGVLTIERHATLAERARAALDETGCANVEVRVGDGSAGAPDRAPFGGISVTAA
                     AEEEPPGPLLDQLAPGAALVCPVRRDGGEELMRFRDGREERVVPVRFVPLVAEPQAE"
     gene            complement(15990..16682)
                     /locus_tag="VF517_14520"
     CDS             complement(15990..16682)
                     /locus_tag="VF517_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8054203.1"
                     /translation="MGSGEDTARLLSQVSLFRELSERELAELAQVAVPRRWLAGESVF
                     REGDSGDTCYVVKNGALRVTRRHSDGRQITLAELREGDIFGELAMFGEGEMRSATVEA
                     VEDSEGVALLAGDLRRTMLAHPSMAVAMLAGLADRIRQVNERLSRQTFQTVDGRVASA
                     LLGQVEAIAGGDGAREVLIRATQAQIAQLSGSSRESASRFLAKLERAGVITTGRGKIL
                     VHEPAALRNYIY"
     gene            16925..17491
                     /locus_tag="VF517_14525"
     CDS             16925..17491
                     /locus_tag="VF517_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054204.1"
                     /translation="MPLRRSIITLCFALALLGAAAPSAGAATKNLWATVNVCDSASHP
                     DDVGIAARMPGGAKGYRMYMRFYVQYLDGEEWRNVSAGGKSPWVLAGSDKFSWIERGW
                     TFSFSPPPAGTSYTMRGFVRFEWRRGKKKVVKRTHRYTSAGHPGTSDAEPKDYSARKC
                     KMTGPPVGTQPQPQPQPEPQPPYQDPIL"
     gene            complement(17511..18293)
                     /locus_tag="VF517_14530"
     CDS             complement(17511..18293)
                     /locus_tag="VF517_14530"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX8054205.1"
                     /translation="MPEGDTIHAAARRVGAALVGQPIESIETPQRRHAMDRWPERLAG
                     RAVRSVDARGKHLFLRFDGDLTVHSHLRMGGWWGVFRRGERWRRSPRRAWLVIRTPSH
                     EVVQFDGPVLELMTDSRTRFDQRLAALGPDILADELDRDEIVRRLRADDPTRGIGDAL
                     LDQRILAGIGNMWKSEGCWVAQVDPWRRVATVSDEELLRVVDATRPLMQASVERGARG
                     FKRDLKVFEKTGRPCERCGTPIRQRGQGDDNRTTYWCPGCQS"
     gene            18376..18933
                     /locus_tag="VF517_14535"
     CDS             18376..18933
                     /locus_tag="VF517_14535"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8054206.1"
                     /translation="MSATASRMTVEDYYDITVEGDRKQLVNGRIVVSDPKPIHDLLRA
                     RVIAALSVWSEAGPGRGLLIAGTDVRIGEHNLLNPDTVWIAEENRPPDLRRYPDRIPD
                     LCVEIRSPSTWRYDIGAKKDAYERGGLPELWLVDDRAERVTVLRRSEPEARTFDVELE
                     LSAGDDLTSPQLPGFALPLEGLFRR"
     gene            complement(18923..>19899)
                     /locus_tag="VF517_14540"
     CDS             complement(18923..>19899)
                     /locus_tag="VF517_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054207.1"
                     /translation="HRGDPPAEPLHAALRERLERGAAFWTDLLVDVGELDGRGVEPVE
                     LQEALWDLVWAGEVTNDAFAPLRAPRLTLAKQREQQARGRRFAGRRRRPGAPQIQGRW
                     SLTGPLFAEAPAHGPRLRAVAELLLERYGIVTRETVLAEGIPGGFAALYGELTNLETL
                     GTARRGYFVEGLGGAQFALPAAVERLRSLRADEPAGALVLAATDPANPYGASLPWPQR
                     DDDSGRRPARTAGAYVVTIDAEPALYLERGGKGIVTLRDGAVDAAGEPADWVREALDA
                     LADHVARGRLQRLALERVDGEPGVGSPYGSLLIELGFRQSPRKLTLTA"
BASE COUNT         2927 a         7348 c         6804 g         2820 t
ORIGIN      
        1 cacccatgca gcatctgtgt gtcggcgaag acgccgagac gccagtacgg gtcgaaccgg
       61 cggaacatgt accgcgccgc gaaaaacacg atcgacgtgc ccgccggcgc gtcgaagtac
      121 tgctgcgacg ccgcgaacgc gggaatcgtt ccgacgttca gcccgttccc ggcccagagc
      181 ccagcggcct cgcgcccggc cgatgggcac cactgcatgg tgcccatctt ctcggaggcc
      241 cgtggcgacc acgacccgtg cgcgccgccc ggcgccgtat cgcaggcgac cacctcgtac
      301 gtcccagccc gggcaacccc cgggccagcc acgagcccgg ccagcgcgag cccgacgact
      361 atcgaaaaac ggcatgcggc agtgcgggcg attccgccca tttgcgcccc ccttcaggtg
      421 cgagtccgtt tgcttccgag gggttgagtc cggggcccgg attcttcgcc ccccgcggtc
      481 agcggagcct gcaccggggt gccgatatac ggcaagcccc ccaaccgggg gcctacccgg
      541 aaactgcggt gatctgagcg gttaacaacc cccaaccggg gagtggcgac accccggtcg
      601 ggtggtctta atcagaccgc gcgcagacgg ccttgaagcc cgcctgcagc cagtcgttcg
      661 cgcccgacac ggcgagcacg ttcgtcacgt tcctccccgc atccgggtag gcgaggcgca
      721 gcccgaccac gccaagcgcg ctccacgcac ccacgcaccg cgtgcacgtg acgagctctc
      781 cgactgcacg gcgcaggccg cggccctccg gcttcgcctc gccctccacg tgttcgacga
      841 acggctcgcg cacccaagtt ccgaccttct ccttcgcgat gaccttcgcc agtgcgaacg
      901 tcgccgccga catcgggatc agctcgtgca gctgcaccgg atccttgcgc gcgcgctcgc
      961 gcgtggcgag gatcaccccg gtgaacaggg ctccgtagac caggttgatc acggcgtagt
     1021 cggacgcgtc cgtcggcggg ctgccatgaa catcgccgcc gttgcgcgcg tgcgcgccga
     1081 gcatgctggt gggctcgcct gccattggct ctggacagta cccggcatcg gtagcgttca
     1141 acacagatgg tcgactggtc gctcgcccgc cagatcgcgc gcttcgcctc tggcgcgggc
     1201 ggctccgccg aggtcgacgc cgacttcccg gccatggtcg ccgacgcgca cggtcacctg
     1261 cgctcctaca cagggctcga gccggtgcag cccatccccc cgccggaggt cgtcgatcgc
     1321 gccgagtggg ccgacgtgaa tctcacctcg ctctgccgga tgctcgagcc ggtctcggag
     1381 ggcctgtcga gccgcctcga gaacgccggg ccgttcgccg gcccgctccg tgcggtgacc
     1441 ggcgccacgc tcgcggccga gtcgggactc gtcatgggct acatgtcgca gcgcgtgctc
     1501 ggccagtacg agctgtcgct gctcgaggcg gaggcgtcgc cgcggctcct gttcgtcggg
     1561 ccgaacctga acaaggcggt ccgcgagctc ggcgccgacc gcgacagctt cctgcgctgg
     1621 atcgtcctcc acgagctcac gcacgtcctg cagttccagg gcgtgccgtg gctgcgcgag
     1681 cacctctcga gcctcatgcg cgagtacatg aggaccgtcg aggtgcggat ggagcgcggc
     1741 gccgcgggtg ggctgccctc gtggccggac ccgcagagga tcgtggaggc gttccgcgag
     1801 ggcggcctag tcgcgctcgt gcagacgcgc gagcagcgaa agatcatgga gaaggtccag
     1861 ccggcgatgt ccgtgatcga gggctactcc gagcacgtga tggacgcggt cggcgagcgg
     1921 gtgctctccg gctacgccgg tctgcgcgac ggcatggagc gccgccgccg cagccgctcc
     1981 gcccccgagc ggatcctcca gcgcctgctc ggcctcgaca tgaagatgcg ccagtacgag
     2041 gagggcaagg ctttctgcga cggcgtggtc gagcgcggcg gcatcgaggc actcaaccgg
     2101 gtgtgggact cgccgcaggc gctgccgacg caggcggagc tgaaggaccc cgcgggctgg
     2161 gtcacgcgcg tgcaggcgct gcccgccgcg gcttaggacc tgcggcagtc ggacggcggg
     2221 cccggggcgg atctcatcga ttggagcctt gctccgtcat ggagtacaga aggcgccagt
     2281 cgatgacgcg gcgggcgggc gggggctaaa ggtctcgccc ccgctgccga tacaccaccg
     2341 gtggccccct ggacccggct gtcgcccctt gcgctggtct tggcgctcgt ctgcctcggc
     2401 gtgacggccg catccgcgtc cgccgccaac agcaaccggc tctacgtaaa cgcgagcgcc
     2461 gaccgccccg ccggcgtcga cgcgtcccgg ctctacgacg tcgccgacca ctccgccagc
     2521 cgctggaagc tgtcggtcga gggcaacgcc agccgcatgc ccggcgtcaa ggacggcacg
     2581 caggtgatcg gcttctcgaa ggccaccgcc gcgcaggcgc tcggcgtgac caccgtctgg
     2641 agccgcacgc tctacaaggt cgtcacgacc cggcgctgct ggcgccagaa cggcaggcgc
     2701 cgctgccaga ccacccgccg ctacgtgaag aacggcgtcg aggtggtcga gaaggacgtg
     2761 cagctcaacc cgtccgtacc gtgggagcag ggcccgtcct acccgtcgcc cacccggttc
     2821 gacctcgagt cgacgatcct ccacgagctc ggccacttcg ccaacccgct gaaggacaac
     2881 cacgtcttcg gctgcgagaa ctcgccgatg atcgactcga tcgcccccgg cgagttctgg
     2941 cgcgacgccg acgactggct gcgctatggc tgctccgcct ccgcgggcgg gcgctccaag
     3001 ctggcggttc ccgccgctcc gcagatgcgg atgctcgtgg tcgagcaccg cctcccgccg
     3061 cgcttcgagc actagcggcg cggaaggctc gcggcgctgg cgagcgcgcg acggagcgtc
     3121 cgcgccggac gacccatgca ccggctgccc gcgcaccgcg acccatgaac cgcgcaccgc
     3181 ggcccgcgca ccccgcccgc ggccagctca gtgccccacg tcacacgcct gaggtgttta
     3241 cgaacacacg ttccggctat attcgtattg tcgttagaac taataacctt agattcgcct
     3301 agcagcccca ccccgggcac cggcagaggg acaaggctca gtccctaccg aagaggcgag
     3361 gagggcaaga caaaatggct cagcagagca ccagcagcaa ccgacgtagc accgccagta
     3421 agcgcagcac gagcagccgc tcgaccagca gcgccaacgg caactccaag cgcaccacga
     3481 ccggcacgca gcgcagcgcc cgcgcgaaga gcgccgcggc cagccgtcag cgcaacaccg
     3541 ccaaccgcca ggccgcccgc acgcgctcga acgcgcgtcg gaccaccacg caggcgcaga
     3601 ccacggcccg tcaggccgcc ggcgctgccg cccagcgggt caaggccgag acgaccgccg
     3661 tccaggccgt cgttcagacc gcccagcgtg gcgtgctgat cccggtcggc gccgctctca
     3721 ccgcgcgtga caacgtggtc gagaccgtcc gcccgtacac ccgcggccgg accagcatcg
     3781 agcgcgaggt cacccggatc cagaagcgcg tgtcgaccaa cctgcgcagg ttcgagcgcc
     3841 gtggcgagac cgcgcgcact cggctccagc gtcaggtcaa gcgcacacgc actcaggtcg
     3901 agcgcgaggc gcgtcagcgt cgcaaccagg tccagcgctc ggtcaagacg gccggcaagg
     3961 acttcggccg cggattcgac cgcatccagg cgggcgtcga cgagctcaac cccgtctcgt
     4021 aggcagcacc acttaacccc cgctccgccg gagagacggc cagcggggga gagctatcgg
     4081 tcctcatacc ccgaccggta gtgcagtatc tctcctccct cgaaccggcg gggcccttgg
     4141 gctccgccgg ttcactttct agagcgatat cgtcacccgc gatgccgtcc gaggaccaga
     4201 tccgcgacgc cctgcgcgtc gtcatcgacc ccgagctgcg ccagaacatc gtggacctcg
     4261 ggatggtccg ctcgatccgc cagccggtgc acggccgcat cgaggtcgtg gtctcgctta
     4321 ccacccccgg ctgcccgatc cgctcgcact tccagacggc cgtggtcgac gccgtgagcg
     4381 ccctcgaggg ggtcgcccag gtggaggtcg gcttcgacgt gctgtcggcc gacgagaagc
     4441 agggcctcca gcagcggctc ggccgccagg ggggcctgcc cagcggcgcc ctcgctcgcg
     4501 tgaagaacgt gatctgcgtc ggctccggca agggcggcgt cggcaagtcc acgatcaccg
     4561 ccaacctcgc cgcggcgctc accgccgagg gccacccggc cgccgcaatg gacgcggacg
     4621 tgtggggcta ctcgatcccc cgcatgctcg gcgtgcacgg caagccgcgc gtgaacgagc
     4681 gccgcaagat cgtgccgctg gtcggcgccg gcggcgttca ggccatctcg atcgagttct
     4741 tcctcgagcg ccaggatcag gcgatcgtct ggcgcgggcc gatgctccac aaggcgatcc
     4801 gtcagttcct cgaggacgtc gagtggggcg agctcgacta cctcctgatc gacctcccgc
     4861 ccggcaccgg cgacgtgtcg atgacgctcg cgcagctcct cccccaggcc cgcttcgtga
     4921 tcgtgacgac gccgcagccg gccgcgcaga acgtcgcgaa gcgcgccgcc gagaccgcca
     4981 tccgcttcga cctcgagatc gccggcgtgg tggagaacat gtcggccttc aacggccccg
     5041 acggcgagcg cgtgacgatc ttcggcgagg gcggcggcca ggtgctcgcc gacgagctcg
     5101 aggtgccgct gctcggcaag gtgccgctgg ccgaggacct gcgcgtcggg gccgacgagg
     5161 gcgagccgct ggtgctcacc gatccggacg ctccggccgc ccaggcgatc ttccacgcgg
     5221 cccgcgggct cgtggccgcc accccgcagg aatcgcccct gctgcaggtg gcgaacgggg
     5281 ggctcgaacc ggccgccgcg ccggtcaccg gcacccccct tccgatggct ccgtaggcac
     5341 cccccgatcg ggggtatttc ggacccccca gattggaccg aaatgcccgc gtagagcgga
     5401 atccgcccgc tcagacggcg gatccgtaga cgaatgctct agctagccgc gaccgggcgt
     5461 tgagcacacg cgcgaagcgc cgatgggaac tccaatgacc tctgttggac gcatgcggcg
     5521 cgaggacggc ttctcggtcg tcgagctcgt catcgcgatg gccgtgatcc tggtcggagt
     5581 gctcggcacg ctggtgctgc tcgaccgcgc gaacgcccag acctcgagcg ccaagacacg
     5641 ccagaccgcg aacgctctgg tgcgcgacat cgtcgagacc gcccagggca cgggctacac
     5701 ccagctcacc ccgacgagcg tcaagtccac gctccagtcg aacggcttcc ccgacgacat
     5761 cccggccagc tcgtcgacct gggagatcaa gcgcaacggg atcgtcttct cggtggccgc
     5821 gacggcctgc atcgtggacg acccgggcga cggcctggcc acgcacgcgg ccgacgccgg
     5881 ctactgctcc gacagccccg gcggcaccgg cgacaacagc ggcgacgact accgccgcgt
     5941 gacgctcaca gtgaccccgc ccaccagcat cgggaccccc gtcacccaga cgaccgtcgt
     6001 cggggccaac cgcatcacca acccgggcgg ctcgggcccg ggcggcggga ccgcgacctc
     6061 caacgacgtc aaggagatga agatcatcag cccgacgctc tactcgggcc aggtcgcgcc
     6121 gtgccagaac aacgcaacct gcaccttccc cgcggccacc accaccgcgg tcacgcccaa
     6181 gtcggtctcg ttccgcgcgg tcaccgccta cacggcacag cgcgtggtgt tcaccgtcga
     6241 cggcctggtc acggccacgc tgaccgggcc agccacgacc ttcgactgga cctggaacct
     6301 gccggattcc cagccggacg gcaactacgt cgtggccgcg cagatcttcg acgccaacgg
     6361 cgtgaacgcg atcagcagcc catcgccgct cgtggtgacg gtcaaccgct acgtgcccga
     6421 ccagaacgcc ttcgctccga ccgccgccgg gcgcaacccg ctctggagca acctgcccga
     6481 gatggagacc tacccgacgg cggacacgtc cgcccgggtg gaccgcgaca tcaccgggtt
     6541 cctggccacc cgcttcctca acggggcggc gaacggaacc gcctgccaga ccttctcccc
     6601 caacgcccgc ggctgcctgg acacgagccc gccgtcgtgc tgcagctcga ccgtgaccta
     6661 ccggatcgcg ccgaccggcg ccaacccgga cggctcgcag cagctgggcc cgaacacggc
     6721 cgcctccccg aacgtgaacg ccgtcaacac gcggcccgtc gcgccggaca acgtgacgat
     6781 cgtgcgcacc ggcaatcgcg tcacgatcac ctgggtcaac ccgtccggct cgggcgaccc
     6841 cgacccgggc gactgcatcg acttctaccg cgtctaccgg agggacctga acgggacgac
     6901 gtgggagtac acggatcgcg ccgaccgcac cacgttcggc aacgccgtgg ctccgtgcgg
     6961 aggtagtggc gagtccagca acaccatcac cctgaccgag gacacgaaca ccgcgaagcg
     7021 ctatcgcgtg accgcggtcg acacccacct cgccgagtcg cccaaggaga acatctcggg
     7081 atgatcgctc gcgcgatcag gaaccggctg cgctctcagc gcggcttcac gctcgtcgag
     7141 gtgctcgtcg cgggctcgct gtccatcatc gtgatcggcg ccgcgctggc gccgttcgag
     7201 gtgctcaacc gcacggggcg cgtcacggcg aaccagaacg actcgcagga caacgcgcgc
     7261 aacgccgtgg tgatgatcac gcgcaacctc cgcaacacga gtggacagaa ccagctcgtg
     7321 aacctggcga gcccgtacga cctcgtgatc gagacggtcg accccacccc gaagccgagc
     7381 ggaagccaga acgcgcgaaa cctgatgcgc gtccgctact gcctcgacac gacgagcggc
     7441 ggctcggtct cgctcacgag cggccgcatc tgggagcaga gcctgcggtg gaccaccgcg
     7501 accccgccca ccacgctgcc ctccgtcaac tgccccgaca actcctgggg caccaagcgc
     7561 gccgtggcga acttcatcac gaaccgggcc acctccacca cgcggcccac cgcggtcccg
     7621 ctgttcagct acttcccgac cggcgcctcg ctcgacatga tcacctcgat ccgggtgagc
     7681 atctactcgg atcgggattg gacccacgct ccacgcgaga ccgagctgac gagcggggtc
     7741 ctgctgcgca atcagaacgg ctcgccgacc gcgtccttca ccgccatccc gggcgtggcc
     7801 ggctcgcgca agatcaccct gaacgcggcg gcctcgaccg acccggaggg gctcccgctc
     7861 acctaccgct ggtgcgacgt gacgacggtc agcacctgcg acgacacgac gcgcatcggg
     7921 acgagcacgc tctacaccta cacggctccc gccgccggca accgcaagat cgtgctccag
     7981 gcgttcgaca tcggcggcct tcagtccacc gccggtcccg tcaccgtggt ggcgccgtga
     8041 ggacgcggat cgtccacgcg ctgcgcgccc gtgcggcgga cgagtcgggc ctggcgctcg
     8101 tgctggcggt catgctcacg atgctgatgc tggcgtccgc gatggcattg ctcgcgttca
     8161 ccgacaacca gaccaagatg accggcaagg agcgcctgcg cgagtccacg ctgacgctcg
     8221 cggagggcgc cctcaacgcg caggccaacc tgctctcggc ggggtggccc gagacctcgg
     8281 acaaggcgtt cgtcccgtgc acgaacaagt cgacgaccgt caggtgtccc gatgccggga
     8341 acctgctgcg cgggttcgcg agcaaggact tcggcaacag cgcgccgatc tcgtggaacc
     8401 tcagcgtgcg cgacaacggg ctcggctcgt tctacgacga cgtggccacc gcaagccagc
     8461 ccgcgtggga ccagagcggc cccgggggcg gcgccccgga cggggtcatg tggctgcggg
     8521 cgcaggccac gatccgcggc gagtcgcgca cgatcgtctc cctgatccgc gccacgccga
     8581 tcgcccgcgc attcccgcgt ggcgtgctca cggccggcca cttccacacc ctcaacaacg
     8641 gcaacaagca gctgctcgac accggcagcg ggccgggggt cctcgcgcgc tgcacggtcg
     8701 gccccggcgg gccggaccgc ggcaacccgt gcctcgacta ccagccgacg aaggggcagg
     8761 tctacccgaa ctcgtgggtc tccgatccgt cgatcccgag cgcgatgagc cccaacgaga
     8821 tcgatctgct ccgtaaccgc gcgaaggccg cgaacacctg gttcagcacg tgcccgtcca
     8881 ccgtgccgac cgccccgctc gtcttcgtgg aggacggttc gtgccagatc aacagcggct
     8941 cgtgggtgaa ctccccggcc gcgccgggga tgctcgtgat gtaccggggc gacctgatca
     9001 ttcagggcag cgccggcttc tacgggctga tctacgcggt gaacgccgac aaccgggccg
     9061 acaacctggt caccgtcacg ggcggcgcgt acgtcagcgg ggcgatcgtg gtcgacggac
     9121 gcggcggcct cgcgatcgat ggaaacggcc tggtgcttca gtacgactcg aacgccttca
     9181 acctcgtgac cacgacgcag accatcaacg tgatcgcgaa cagctggcgc gagctgaacg
     9241 ggcactgacg cccgggcggg cgcccccgcg gcctactgct tggacttgta gtagtccgcg
     9301 ttgacctgga tgtagttctg ccactcgtcg gggagctggt cctcggcgaa gcaggcgtcg
     9361 accgggcatg cctccacgca cgcatcgcag tcgatgcact cgtccgggtc gatgtagagc
     9421 atcttgacgt cgtcgtagtc gggctcgtcc ggcgtcgggt ggatgcagtc gaccgggcag
     9481 acctcgacgc acgaattgtc cttcgtgtcg atgcagggct cggcgatgac gtaggccatg
     9541 tggtcggcgt ttctccttgg ttcggcggga tccccgcgca cgtaaaacgg gcgcgagcgc
     9601 gcatcttatt acgctgcgtg cgtgatcctg ctgaccggcg cgaccggaaa cctcggctcg
     9661 gcgctgctgg cgcgcctcgt ggccgaccgc cagcgcgtcc gctgcttggt ccgcgacccc
     9721 cgcggcctgg ggcccgaccg ggtgcgggtc cagatcgcgc tgggggacct cgccgatccc
     9781 gcctcgttcc gcaacgccct gcgcggggtc aagacggtgg tgcacctggc ggcctcgatc
     9841 cgcgaccagc cgcgcgcctc gatcgaggag ctgaacgccg tcgcgaccct gcggctcctt
     9901 cgggcggcgg agaaggccgg ggtcgagcgg ttcgtgttct tctcggcact cggcgcctcg
     9961 ctgcacgagc gcgcgcgctt cttccgggcc aaggcgctcg ccgagcaggc cgtcgaggac
    10021 gcggagatcc cgagcgtggt cttcgccccc tcgatcgtct actcgccgcg cgacccgtgg
    10081 gtccgcctgc tcgagcgcat gtcgctgctg ccggtgatgc cgatctcggg ctccgggcag
    10141 gctcgctacc agccgatctg ggccgacgac gccgccgcct gcgtcatggc cgcgctggga
    10201 gccgacggcg cggcccacac gcgctacgag ctggccgggc cgcagacgct ctcctacgac
    10261 gacatcgccc tcgagacgct gggcgcgctg cgcaggcgcc ggcggctcgt gcacgtgccg
    10321 ctgccggtgg tccgccgcgg gctgcgggcg gtcgaggcgg tggtgggcgc ccaggcgttc
    10381 gccgtctggg aggaggccga gctgatggag ataccgatga ccacgccgcg cgggacggcc
    10441 gacgccgagg cgctcggcgt cagtccgcga ccgatgcgtg cggtgctggg agcccgttag
    10501 cggtctccga gccgacgtag gcgaccatcc ggttggtcag cgctcccacg aggcggaact
    10561 cgtccgcact tggcggcggc gtgtcctgga ggcgctcgcg gatcgcggag acgatgtcgg
    10621 gcgcgccctg ctctgcgatc cccgcggcga cccgggcggc ctcgcgcacc agctcgggct
    10681 cgacgctcca gacggtctcg aaccggcgct cgtagtcccg gatcgcgtcc tggccaggcc
    10741 gctcccaggc ggcgaggaag gcggagtagt ccggcgcgtc gcacacgagg gcccactcgc
    10801 ggcagagcgg gtgctcgtgc tcgatcggga cctcgtgcgg cccgccgtcg ggatcgcgcc
    10861 gctcgtcgaa ctcggcgaag acgatcgccg cgtgcgcggt gcgcgtgagc tcgcgccagc
    10921 gcgcctcggc gtggcggaaa aagctgggcc gctggaagct gccgaacagg accgggcgct
    10981 cggcgcgggc gacgcactcg tcctcgatcg cgtggctgag cgcgatcagc gtgcgcttgg
    11041 gcagcaggta cgggatcagg tcggggcgcc ggcggcggac gccggcgtag atcgacggct
    11101 cgacctcggc ggtggcgttc agcgcgcggt cgatggccgc ggggatcgag agtccggcgg
    11161 cgcggtcgcg cgcaacgcgg cgcacccgct cgacgtcctc gtcgctgtag cgcctgtggc
    11221 cgctgtcgag gcgccccggc tcgggaaagc cgtagcgctg ctcccacatg cggagggtcg
    11281 cggcgttcac gccggtgcgc gccgcgacct cgcggatggt caggtcgttc gccacccggg
    11341 ctacgccgcc gccgccaggt tctcgcgggt cttgcgcagg ccgagccgca ggcggctctt
    11401 ggcggtgccc agcgggatgg cgctgacctc ggcgatctcg ctcgcggtca tgccgcggcc
    11461 gtaggcgagc agcagggcgt cgcgctgctc ggcggggagg tgcctgagcg ccccggccag
    11521 cgtgcgcgag cgctcgcggc ggatgacctg ctcgtcggcg ctctcctcgg caccgcggcc
    11581 ggtcacgcgg acctcctcct tggtgcgctc cacggcgttg tctcgggcgc tgcgcgtacg
    11641 ccagcggtcg agcgcgcggc tgcgcgccat catcgtcacg tacgcgctga gcgggccccg
    11701 cgcggcgtcg tacgcgctcg gcctcagcca gagctgcatg aacacatcct gcacgacgtc
    11761 ctcggcggcc gcctcgtcgc gcagcacgcg gttggcggcg gcgacggcct gtggtgcgag
    11821 ctggcggtaa gcgtcatcga actggcgcgg gtccttcagg ttgggcgaca tctggattct
    11881 tccccttgtg gcggttttgt tcaacaccgt gttgaagaat atatcgcctt gtggaggaaa
    11941 acctaaacaa gctgtacagg ctggcgcgaa gccgcgccgg gccgcgcgaa gccgcgccgg
    12001 gccgcgcgac accacgcaga ggccgcgcgt gctcgcgcac ccggggacct cggaaccggg
    12061 cccaggaacg acgaaggccc ggcgcaatgc cgggccttcg ccaacagatt ccggtcggtg
    12121 gaccgggagc tacatcatcc cgtccatacc gccgggcatg ccgccgccca tgccgccggc
    12181 gccgtccttc tccggcgcct cggccacgat cgcctcggtc gtcaggatgt tcttcccgat
    12241 cgacgcggcg ttctggagcg ccgagcgcgt gaccatcgcc gggtcgatga ccccggcctc
    12301 gacgaggttg acgatctcgc cggtgtccgc gttgaggccg tgaccggcct tggccttgcg
    12361 gacgtcgttg accacgaccg agccctcgag cccggcgttg ttggccagct ggcggacggg
    12421 ctcctccagg gcgcggagga tgatccgcgc accggtctgc tcgtcagcgt cgtcgatcgc
    12481 gtcgaccttg accgcgttcg ccgcacgcag cagcgcgacg ccgccgcccg gcacgatgcc
    12541 ctcctcgagg gccgcgcggg tggcctgcag cgcgtcctcg acgcggtgct tcttctcctt
    12601 catctcggtc tcggtggcgg caccgacctt cacgaccgca acgccgccgg ccagcttcgc
    12661 gagccgctcc tggagcttct cgcgatcgaa gtccgagtcg gtgttctcga tctcgttctt
    12721 gatctggttg atccggccct tgatcgcctc ggcctcaccg gctccgtcga tcacggtggt
    12781 gttgtccttc gcgaccacga cgcgacgcgc gcggccgagc tgcgagatct gcgtgttctc
    12841 gagcttgagg cccatctcct cggtgatgac ctcgccaccg ctcaggatcg cgatgtcctc
    12901 gagcatccgc ttgcggcgat cgccgaagcc cggcgccttg acggcgacgg cgctgaacgt
    12961 gccgcggagc ttgttcacca cgagggtggc gagggcctcg ccctccacgt cctcggcgat
    13021 gatcagcagc gacttgccgg cctggatgac ctgctccagc acgggcagca ggtcgcggac
    13081 cgagccgatc ttctggttcg cgatcaggat gtacgggtcc tcgaggacgg cctccatgcg
    13141 gtcctggtcg gtgaccatgt acggcgagat gtagcccttg tcgaactgca tgccctcggt
    13201 gaactcgagg tccatgccga acgtctggcc ctcctcgacg ttcacgacgc cgtccttgcc
    13261 gaccttctcg atggcgtcgg cgatgatgtc gccgatctcg tcgtcggcgg ccgagatcgc
    13321 ggccacgcgg gcgatctggt ccttgcccga gatctccttc gcctgcttgc ggatctcgtc
    13381 cacgacctgg tcgacggcct tctcgatgcc gcgcttgagg ccgagcgggt tcgcgccggc
    13441 ggccacgttc ttcaggccgg tgcggacgat ctgctgagcg agcacggtcg cggtggtcgt
    13501 gccgtcgccc gccacgtcgt tggtcgccgt ggcgacctcg cgcacgagct gagcgccctg
    13561 gttctcgaag acgtcctcga cctcgatctc acgagcgatc gtgacgccgt cgttggtgat
    13621 ggtgggggcg ccgaacttct tgtccaggac gacgtagcgt cccttcgggc caagggttac
    13681 cttgaccgcg ttggcgaccg cgtcgacgcc ggcctcgagg gccgagcgtg cgtcctggtc
    13741 gtacttgagc tccttgtgag ccataagttc cgtttcctcc gtctttctcc gtagaagtgc
    13801 taggcgacct tcgcgagtac gtccgactcg cgcaggacga gcaggtcctc gccctcgatg
    13861 ttgatctcag tgccgccgta cttggagtag aggacctcgt ccccctccgc gacgtcgagc
    13921 gggatgcggt tgccgccgtc ctcgctgagc ttgccgtcgc ctacggcgag caccttgccg
    13981 cgctgcggct tctccttggc cgtgtcggga aggacgatgc cgctggcagt cgtctcctcc
    14041 tcctcgaccg ccttgacgat cagacgatcg cccaggggct tgagcttcat agacaaaacc
    14101 tccgtttgag tccctcggaa atgcgtttgg cactctagca acgagagtgc caaagctgca
    14161 acgcgatccc gcgtggggag cggcggtgcg ccctggcggt gccctagatg ccgagcgcgc
    14221 agttgctcgc gccccagccg ccggcagcgg cgaagacgag cgacgcgagg ccggcgaacc
    14281 ccagcacgcc cgcggcccag cgcgggcgcg gcagggccag cagcacggcg agcacgaccg
    14341 ctccgaaggc cacggccgcc agcagcacga ggtacccgcc tgagtcctcc acatcgcagg
    14401 cacggcgctt cacgccgtcg cccaggagcg atgcgaacac gccctggacc agggcgaatg
    14461 gcagcgccgc gaagaggacg cccaggaccc tggacgtctg cttgcgatcg cgctcgctgc
    14521 gcgccaccgc cggccccgcg ggctagttct gggcgggcgc cgcggggcgc atccgctcgg
    14581 tgatctcctc cgggcggtcg tagatcgccg agaagcggac cacggtgacc gtgtcgtcgg
    14641 tggagaccgg gcggccgtag atctgctcga ggaaatgggc cgtctcctcg acgcggcgga
    14701 actcggggtt gtcgtgttcg atgtcgcgcg gggagagctc cgacaccttc ttgacgtcga
    14761 ccgcgtcgat gaccgcctcg aagatcttct cgcgcgggga gtgctggaag cccaccgtga
    14821 tcaggacgac ctcgcccttc tgatacttgc ggctcttgtc gccgagccgg atcgtggcgg
    14881 tcttgcgccc gcgcttgagc tggtccacga agaccggcga gtagaagttg aggacgaaca
    14941 cggcggccct cagactaccg agccgcaccg agcgccgaca gggcggcgct cgccatgtcg
    15001 cgctcgccgc ggtaggcgag cagccgcggg gcgtcctcga gcggcaccca ctcggccgag
    15061 tcgacctcgt gatcgtggtc ggcgacgctt cccgagctgt agcggaacag gaagaacgcc
    15121 acgttcttca tcacccggtc accgtcgcgg cggtaccagt agcgcacgtc gccgagcttc
    15181 tccaccagat cgccggtcac cccggcctcc tcgcgcacct cgcgggcggc ggcctcggcg
    15241 gagctctccc cagggtctat gtggcccttc gggagcgcga gcacggttcc ccccttcacg
    15301 cggaccgccg caacgaagtc gcgcccgcgc atgcggcgca cgcacacccc gccggccgag
    15361 aactcgcgga tcactcggcc tgcggctccg ccacgagcgg cacgaaccgg acgggcacga
    15421 ccctctcctc gcggccgtcg cggaagcgca tcagctcctc gccgccgtca cgcctgacgg
    15481 ggcagacgag ggccgcgccc ggcgcgagct gatcgagcag cgggcccggc ggctcctcct
    15541 ccgcggcggc cgtgacggag attcctccga acggtgcccg gtcgggcgcg ccggccgacc
    15601 cgtcccccac gcgcacctcg acgttggcgc agcccgtctc gtccagcgcg gcgcgggcgc
    15661 gctcggcgag cgtggcgtgg cgctcgatcg tgagcacacc gcggcagcag cgcgacagca
    15721 cggcggcgcc gtagccagag ccggtcccga cctcgagcac cagctcctcg ccggtcaggt
    15781 cgagcagcgc ggtcatgcgg gccacgatcc aaggctgcga catcgtctgc ccggcgccga
    15841 tcggcaccgc gccgtcgcgg taggcgcggt cgcgctcctc ctcgggcaga aacgcctcgc
    15901 gggggagctc cgccatcgcc gccaggacgc gctcgtcggc gattccgcgc tcgcggagct
    15961 ggccctcgac cattcgcgcc cgcgcgtcct cagtagatgt agttgcggag cgccgcaggc
    16021 tcatggacga ggatcttgcc gcgcccggtg gtgatcaccc cggcgcgctc gagcttcgcc
    16081 aggaagcggc tcgcgctctc gcgcgaggag ccggagagct gcgcgatctg tgcctgcgtg
    16141 gctcggatca gcacctcccg cgcgccgtcg ccgcccgcga tcgcctcgac ctggccgagc
    16201 agcgcgctcg cgacgcgccc gtcgacggtc tggaaggtct gccgcgagag ccgctcgttg
    16261 acctggcgga tccggtcggc cagcccggcc agcatcgcca cggccatcga cgggtgcgcg
    16321 agcatcgtgc gccgcaggtc gcccgccagg agcgccacgc cctcgctgtc ctcgaccgcc
    16381 tccacggtgg ccgaccgcat ctcgccctcg ccgaacatcg ccagctcgcc gaagatgtcg
    16441 ccctcgcgca gctcggccag ggtgatctga cgcccgtccg aatggcggcg cgtgacccgc
    16501 agggcgccgt tcttgacgac gtagcaggtg tctccggagt caccctcgcg aaagaccgac
    16561 tcgcccgcga gccagcgccg tggcacggcc acctgagcca gctccgcaag ctcccgctcg
    16621 gacagctcgc ggaaaagcga gacctggctg agcagccttg cggtgtcctc cccgctcccc
    16681 attacgagga ttatcgcgta ccctgaggtc aggtccgcac atggacgtgc ggcgcggaag
    16741 gaggaacgaa tggcacggac gctcggactg cgcgctctcg cgtgggtctc gctgctcggc
    16801 gcggtcgccg ctgtggcgca tacggtccag acgacgctgg cgctctgacg cgcctgcgct
    16861 cacgcaactt agacgcggct taggggtcta ggtagggtcg gcgctccggc ccgacgtact
    16921 gcccatgccc ctacgccgct cgatcatcac gctctgcttc gccctggccc tgctgggggc
    16981 agccgcgccg tccgcgggcg ccgcgacgaa gaacctgtgg gcgacggtga acgtctgcga
    17041 ctccgccagc caccccgacg acgtcgggat cgcggcgcgg atgcccggcg gcgccaaggg
    17101 ctaccggatg tacatgcgct tctacgtgca gtacctggac ggcgaggagt ggcgcaacgt
    17161 gtcggccggc gggaagtcgc cgtgggtgct ggccggctcg gacaagttct cgtggatcga
    17221 gcgcggctgg acgttcagct tcagcccgcc ccccgccggg acctcgtaca cgatgcgcgg
    17281 gttcgtgcgg ttcgagtggc gccgggggaa gaagaaggtc gtcaagcgca cccaccgcta
    17341 cacgagcgcc gggcacccgg gcacgtcgga cgcggagccg aaggactaca gcgcgcgcaa
    17401 gtgcaagatg acgggcccgc ccgtcgggac acagccgcag ccccagcctc agcccgagcc
    17461 gcaaccgccg taccaggacc cgatcctcta gttcggaaga tccgcgccgg tcaggactgg
    17521 cagcccgggc accagtaggt cgtccggttg tcgtcgccct gaccgcgctg gcgaattggc
    17581 gtgccgcagc gctcgcaggg gcggccggtc ttctcgaaca ccttgaggtc gcgcttgaac
    17641 ccgcgcgcgc ccctctccac cgacgcctgc atcagcggcc gcgtcgcatc gacgacgcgc
    17701 agcagctcct cgtcgctcac ggtcgcgacg cgccgccacg ggtccacctg cgcgacccaa
    17761 cacccctccg acttccacat gttcccgatg ccggccagga tgcgctggtc gagcagggcg
    17821 tcgccgatgc cgcgcgtggg gtcgtcggcg cgcaggcggc ggacgatctc gtcgcggtcg
    17881 agctcgtccg cgaggatgtc cggaccgagc gcggcgagcc gctggtcgaa gcgcgtgcgc
    17941 gagtcggtca tcagctcgag taccggtccg tcgaactgga ccacctcgtg gctcggggtg
    18001 cggatcacga gccaggcgcg gcgcggggag cggcgccagc gctcgccgcg gcggaacacc
    18061 ccccaccagc cgcccatgcg caggtgcgag tggaccgtga ggtcgccgtc gaagcgcagg
    18121 aacaggtgct tgcccctcgc gtcgaccgag cgcacggcgc ggcccgcgag ccgctcgggc
    18181 cagcggtcca tcgcgtgccg gcgctgaggc gtctcgatcg actcgatcgg ctggcccacg
    18241 agcgcggccc cgacgcgccg cgcggccgcg tgaatcgtgt cgccctcggg catggcaacc
    18301 gaggttaggg ggacgaaaac ggccgggcag gggggcagaa actcagggcg ggctggctta
    18361 tcgtccgtgt ggagcatgag cgcgacagcg agccggatga cggtcgagga ctactacgac
    18421 atcacggtcg agggagaccg caagcagctc gtgaacgggc ggatcgtcgt gagtgatccg
    18481 aagccgatcc acgacctcct tcgtgcgcgg gtcatcgcag cgctctcggt ctggtcggag
    18541 gccggtcccg gccggggcct gttgatcgct ggcaccgacg tccgcatcgg cgagcacaac
    18601 ctcctgaatc cggacaccgt gtggatcgcg gaggagaatc gtccgccaga cctccgccgg
    18661 tatcccgacc ggatccccga cctctgcgtc gagatccgct ctccgagcac gtggcgctac
    18721 gacatcggcg cgaagaagga tgcgtacgag cgcggcgggt tgccggagtt gtggctcgtc
    18781 gacgaccgtg cggagcgcgt gaccgtgctg cggcgctcag agccggaggc gcgcacgttc
    18841 gacgtcgagc tggagctcag cgccggcgac gatctcacct cgccgcagct acccggcttc
    18901 gccctccctc tggaaggcct gttcaggcgg taagcgtcag cttccgcggc gactggcgga
    18961 agcccagctc gatcagcagc gagccgtagg gcgagccgac ccccggctcg ccgtcgacgc
    19021 gctccagcgc cagccgctgg aggcggccgc gcgcgacgtg gtcggccagc gcgtcgagcg
    19081 cctcgcgcac ccagtccgcc ggctcgccgg ccgcgtcgac cgcgccgtcg cgcagcgtca
    19141 cgatcccctt gccgccgcgc tcgaggtaca gcgccggctc ggcgtcgatc gtgaccacgt
    19201 aggcgccggc cgtgcgggcg ggccgccgcc ccgagtcgtc gtcgcgctgc ggccacggca
    19261 gcgaggcgcc gtacgggttc gcggggtcgg tggccgcgag cacaagcgcg ccggcgggct
    19321 cgtccgcgcg cagcgagcgc agccgctcga ccgccgccgg cagcgcgaac tgggctccac
    19381 cgaggccctc cacgaagtag ccgcggcgag cggtgccgag cgtctcgagg ttggtcagct
    19441 ccccgtacag ggcggcgaag ccgccgggga tcccctcggc cagcacggtc tcgcgggtca
    19501 cgatgccgta gcgctcgagc agcagctcgg ccaccgcgcg caggcgcggc ccgtgcgccg
    19561 gtgcttcggc gaacagcggc ccggtgagcg accagcgccc ctggatctgc ggcgcgcccg
    19621 ggcgtcggcg gcgcccggcg aagcggcgcc cgcgcgcctg ctgctcacgc tgcttggcga
    19681 gggtgagccg cggcgcgcgc agcggggcga acgcgtcgtt ggtcacctcg cccgcccaga
    19741 cgaggtccca gagcgcctcc tggagctcga ccggctccac cccgcgcccg tcgagctcgc
    19801 cgacgtcgac cagcaggtcg gtccagaacg ccgcgccgcg ctcgaggcgc tcgcgcagcg
    19861 ccgcgtgcag cggctcggcc ggcgggtccc cgcggtgcg
//
LOCUS       DASVPL010000264        14173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_70918, whole genome shotgun sequence.
ACCESSION   DASVPL010000264 DASVPL010000000
VERSION     DASVPL010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14173
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70918"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1752)
                     /locus_tag="VF517_14545"
     CDS             complement(34..1752)
                     /locus_tag="VF517_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054208.1"
                     /translation="MSKHAAVAVAALFMVLAAPASAAPTPAWTQKADEIAAPWPGLQE
                     ASGSFRDYVLVRDPSDQRDDYGDAILGYGLLLNATRRNDPALADAGLRALEYSLERAA
                     RSPSTQVFHQLATVSAYNLARQRYADHPVFQRARSRWEDVLRRIEVYRIGRQKVTNKS
                     IVEAILLLELVRSGLTSTQPGAALADPAATLALVKHFLASDLPEAARPFQIDGRTVLG
                     DMPLLPPAYHGLSIGMLARTIELLGDEAPSAARTLLRRGVAASLAMAGPDGEVAYHGR
                     SQAQAWTLTLTAYGAERSAQHGLAERTVGRLIDAYPTGPEGFLVTPSMAQGIDAAIPG
                     VDEYVAAASYSGLTLMALEWAIAAGGGDAGGAQPSGAHVLGTGTGAWATSRRGDVWFA
                     VKRTRTSPRDLRYDFGLMALKVRGGGTWGDAMPLRPRTVSGVDSVGPVLTAGGSVGYP
                     EGTELRLGRAGAIVVRGGFRTRTGRWLRRGVTFTFAPVACGVRLTFAARAGDRFAYSG
                     FFLDTPRRSGRTVRDGDQQLRFDTAPSVGVRAGYASGSDVGLTRAVARWKRTRAGTAS
                     IETCAR"
     gene            1757..3604
                     /locus_tag="VF517_14550"
     CDS             1757..3604
                     /locus_tag="VF517_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054209.1"
                     /translation="MRRATLVPILLAVLAFAPAAAAKAPSTWGPLAEEIAAPWPGLQN
                     ENGTYQDYVYGGDVSFCLVRSCKPGLGNARYGEAVLGYALLRTGVRTGDGALMDTAVR
                     SLTWVAEHPEYRDTLQTNFETWALASAYNLARTRIASRPLFRQNRASWERWLKEVKPL
                     LLRAGSRRYFNHHLVEAVATYELKRTRLRSKARGAVLNPDVYKGVERRADQTVNEEVH
                     LVARPTMLRSGGAVAALLSDRPEYPLAYQGLSMAFYARALELMGSRATERSRELLRRL
                     AHASWLLTAPDGDLAYAGRSQEESWALPMTAYGVEVAARLPGVGAGSAARWRALADRT
                     VERLRTAYPVGPQGMWIIPALARDPQRGLPGVDPYAGAAIFNGLTLIYLEAARDEAER
                     ARRGVGRIASDAGGPRRLGRADDTFVTLRRGGVWFAIRQSRDLQRRLDDLRNDFGLVA
                     LKRLEDGRWRDVIPVRPRVESEDRRAADSAGPRLRGGVPTGERIAADSDSVTVTGGWR
                     LPDGTFVRRGTKFRFEAIGCGVRQRVPVAAGDAIEYDVLAVRDPEGISVDGARIADGQ
                     QVVTFSEAPAEVKMLPGYSSGSHPVITRVRATFPAAAGARTLDITTCDR"
     gene            3635..5119
                     /locus_tag="VF517_14555"
     CDS             3635..5119
                     /locus_tag="VF517_14555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8054210.1"
                     /translation="MTTSTTPQKSSRWAALVVLCTGMLMIVLDATVVNVALPTIQDDL
                     GFSQSSLAWVVNAYLIAFGGLLLLAGRLGDLIGRRRVFLVGLAIFTTASLLCAVAQSQ
                     GVLIGARFVQGIGGALASAVILGMIVTLFPEPREQAKAIGVFGFVASAGGSIGLLAGG
                     VLTEAINWHWIFFINLPVGVATALLARRFVEADEGIGFRQGADVPGAVLVTVALMLGV
                     YTILEVSDRGWVSLHTLGLGGISAALVAVFVARQARIPNPLMPLRLFRSRNVVTANTL
                     QILLVAGMFGMFFLGALYLQRVLGYDPLEVGLAFVPVTLVMGTLSLRFSERAAMRFGP
                     RNTILPSLVLIAAGLALFARAPVEGTYLTDVLPPMLLIGAGGGLGFPALMMLAMSGVN
                     PEDAGLASGLVNTSMQVGGAIGLAVLATLAATHTDSLRADGESTAAALTGGYQVAFVV
                     GAGLAVAALALALVVLRPEPAGAAAGADAEREGQSTSEPAAEAA"
     gene            5119..5808
                     /locus_tag="VF517_14560"
     CDS             5119..5808
                     /locus_tag="VF517_14560"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX8054211.1"
                     /translation="MAAAVAFPNESAEYRAARERLLAAEVEERRAHEALAAARRELPP
                     GGEVPEDYVFERAGADGTPEPVRMSELFARGDTLAVYSFMFGPDRDAPCMGCTSMLDG
                     IDGATRHIVERINFAVVAESPVPRLLEFAERRGWSWLPLLSTAGNSYNRDYHGKDPDG
                     YDQPMLNVFQRTGATIRHFWGSELLYQPMEPGQEMRHLDSIDLFWNMFDFTPGGRGED
                     SPPDLAPASAG"
     gene            complement(5805..7043)
                     /locus_tag="VF517_14565"
     CDS             complement(5805..7043)
                     /locus_tag="VF517_14565"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX8054212.1"
                     /translation="MADVQPLRSLRYDLNSVGSLDAVAAPPYDVIDDALRAELVAKSP
                     FNVVEIDLPQGNGGDPYLHAETTFEAWRQQGVIVKEREPAIWPIEQEFVDPYGQSHVR
                     GGFLCRVRVEDYGPGRIRPHERTQPGPKEDRLRLTRACRANLSPIFSLYAGDPVSFGD
                     LGATWGSYTDGSGATTRVWRCGDEEQLAAVRDALADRELLIADGHHRYETARVYAEEI
                     GGEGEHRYCLMALCAMSDPGLTVLPTHRLLTRIDPEKREALRAAVERDFEVEPIERDD
                     LEPPAGDRIELGYYDGHHKRPLRLRLRDQAIADRALASKPPPYRRLDTAFLEQAILMD
                     TLGLTEDDIARQNGIGYAKRADHAIEAVESGAAEAAFFMGATPVELVQEVAEAGESMP
                     PKSTFFYPKIPTGLVFNPLD"
     gene            complement(7108..7638)
                     /locus_tag="VF517_14570"
     CDS             complement(7108..7638)
                     /locus_tag="VF517_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX8054213.1"
                     /translation="MNLSLRHARIAVLVAAILALLVPATASACPGENEEPTAVSLDVA
                     ERATFCLINRERTTRGIRRFRRNPRLDLASVRHARSMAARNFFEHGNFVGRIRGANYL
                     RGARGWRLGENIAWGSGTLSTPVEIVDAWMHSPGHRANILQRGFREIGIGISRGTPNR
                     SYEDGATYATDFGTRG"
     gene            7807..7880
                     /locus_tag="VF517_14575"
     tRNA            7807..7880
                     /locus_tag="VF517_14575"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7841..7843,aa:Pro,seq:tgg)
     gene            complement(7881..9260)
                     /locus_tag="VF517_14580"
     CDS             complement(7881..9260)
                     /locus_tag="VF517_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX8054214.1"
                     /translation="MTRRPIRLSPADAAAPLFEAIRREADVTAAFPPAVEAEAEAAAR
                     VPVAGERSELPFVTIDPPGSRDLDQALHIERRGAGHRVSYAIADVAAFVGPGGALDRD
                     VHERGVTVYAPDAKVPLHPPVLSEGAASLLPGEWRPAVLWTLDLDATGELVAAAVARR
                     LVRSVAQHTYEDVPPELEPLLREVGERRLAIERERGGVRLAVPEQEVEREGDSWTLRY
                     RVPLPSEEHNAQVSLLTGMAAADLMLRAGVGILRTQPEPEARAVERLREQARALRVEW
                     PAGLSYPEFVRTLDPAVPAHAAVMHEATGVGHGAGYTAFDGAPPEEPEHFAIAAPYAH
                     ATAPLRRLQDRYVSECCLAAAAGGPVPDWVRAGLPELPGAMAAADKRARAVERAVVDV
                     VEALLLQGREGERFEAVVIDEGTVQLRDPAVRGKLDGDCPSVGTELTVRLERADPATR
                     TVEFSPASG"
     gene            9311..9769
                     /locus_tag="VF517_14585"
     CDS             9311..9769
                     /locus_tag="VF517_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054215.1"
                     /translation="MTTKADFNADEWSTITEAPMLAALRTVAAGRGGTIRESIAVGKV
                     YAEAKQGQETNPLVAELVGSPPSLDPQKVQSEGGFEAVSDARLREALRLLGEKATPDE
                     VEAYKGFVMSLARAAAEAHKEGGFIGIGGKAISAEEQAALDQIESLLQGS"
     gene            complement(9842..10363)
                     /locus_tag="VF517_14590"
     CDS             complement(9842..10363)
                     /locus_tag="VF517_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054216.1"
                     /translation="MTLSRQGRLTVLTTILAIAFAAVVGPANAGGGKDLAEGNGTTPD
                     PNASGTDFRFAFNAIGDTTSAKGTLRLKNPSTGTQVRGEVICLQTEGPQRAVIAGRIT
                     SISGPAPYGAPNVEEGDAFLLFAEDNGKNRSESDRLAVFLFLGEPPTTELCDADQSGP
                     APITSGHITIRDR"
     gene            10486..10806
                     /locus_tag="VF517_14595"
     CDS             10486..10806
                     /locus_tag="VF517_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054217.1"
                     /translation="MRRLGAAASVALVAAAPYVIFRSTFEDAATDFRWELVYLISGWG
                     PFVLMGIGVLWFVPVVLSMHRTGYSRLYLRPVTRHACEAWGLVTYTLGFMLAIQTSQI
                     AGLF"
     gene            complement(10803..12461)
                     /locus_tag="VF517_14600"
     CDS             complement(10803..12461)
                     /locus_tag="VF517_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054218.1"
                     /translation="MKRALLATAFAILVAAAPAGAAPLPDVVADDLAPARGTNLVRAI
                     GLPDGSGARMVARGSVTRSLPRRPWSVSVDLRPSDHSTVVVELGADKLVFDRNGDGTT
                     RVRGPGIAPTALPEQGYAGRDWRHLEIAGGSDPLLAIDGQPIENELAATGSTVRVRVV
                     EGSAQATGLVATRRDDRRAALLHRLAELHARTPLGRFPVGIGDDDSLLRFTRDWTDGF
                     WPGMLWLAADMTAPSAPFADLARAATDRHRGRERDAIHDQGFRYLLSSAAAHDRECGG
                     RARSSSACRRLRASAVTAANTLLELAAGNEAVGTIPTVGSEGDCRRCASADESETIID
                     SLMNLGLLGWAWRERGDARYREVALRHARAVERLLVRPDGSTAQSVIASRADGHVVSV
                     GTRQGYSDTSTWSRGQGWALYGFADTGARFRDRGLLAAAERVAGYVAGHLPASGIPPY
                     DYDAPAPAPTDTSAGTITAAGALRLAAACESIARACEDGPRWRRLGLRMLSASLGRVS
                     RLPPIGMLGDQVYSLGGGSTWDDRGEYAFGLYYAFDAIRRARSG"
     gene            12535..12607
                     /locus_tag="VF517_14605"
     tRNA            12535..12607
                     /locus_tag="VF517_14605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12568..12570,aa:Arg,seq:tct)
     gene            complement(12608..13573)
                     /locus_tag="VF517_14610"
     CDS             complement(12608..13573)
                     /locus_tag="VF517_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054219.1"
                     /translation="MSSPTRFSCRANSPLSPKRRTIPGRDFVANNGLAGLLVGFVFKP
                     PIVELTRSPVPTVSWTITASVTVRAGGLASDTKSLSVPVELGQLEIPKFIALFRHKNF
                     AAFEDPDTPGFVLMLIPHHSILQGISEPLNDLLDRIHEALRPLRSLVGIAAFLTGIDT
                     LKQALASQPLVRVKRGSEDNLEDIHMRVDKFLGSTWLEKDLRPNDRVSALILMGPCGT
                     RVGCFDDEDFTTEHGEWGFHVRTGPEMVTIISDLSDGKPPPTFPEEDPNDPRSQRLFI
                     LRRGGASFADNMTSVLFDPEPSDQEKELPDPCLAVEVPGSGGPIL"
     gene            complement(13539..13952)
                     /locus_tag="VF517_14615"
     CDS             complement(13539..13952)
                     /locus_tag="VF517_14615"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8054220.1"
                     /translation="MPGAGDTLENPATGERITFLRTAADTGGDLLEMEDVWVRPDHAT
                     APHLHPRMEETWEVVSGRAGFRIGDDELEAGPGERVVAPRGVVHSAWNLSGGETRLRI
                     TMRPALRWEQFVERLFTAPGPELLREFADEIQLPG"
     gene            13974..>14173
                     /locus_tag="VF517_14620"
     CDS             13974..>14173
                     /locus_tag="VF517_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8054221.1"
                     /translation="MPTSLVTGGAGFLGSHLCDYLIERGHRVLCVDNLETGSLQNIAH
                     LRGPEFEFLHLDITHHYEVAEPV"
BASE COUNT         1838 a         5118 c         5245 g         1972 t
ORIGIN      
        1 gcccggctgc ggtctgccgg tctgcccgct tccctagcgg gcgcaggtct cgatcgaggc
       61 ggtccccgcg cgggtgcgct tccagcgcgc gaccgcccgg gtcagcccga cgtccgaccc
      121 ggaggcgtag ccggcgcgga cgccgacgct cggcgccgtg tcgaagcgga gctgctggtc
      181 gccgtcgcgc accgtgcgcc ccgagcgcct cggcgtgtcc aggaagaacc cggagtaggc
      241 gaaccggtcc ccggcacgcg ccgcgaacgt gagccgcacc ccgcacgcca cgggcgcgaa
      301 cgtgaacgtg accccgcgcc gcagccagcg cccggtgcgc gtgcggaacc cgccgcgcac
      361 cacgatcgcg cccgccctac ccagccgcag ctccgtcccc tccgggtacc cgaccgaccc
      421 gccggcggtg aggacgggcc cgaccgagtc cacccccgac acggtgcgcg gccgcagagg
      481 catcgcgtcg ccccacgtgc cgcctccgcg caccttcaga gccatcagcc cgaagtcgta
      541 gcggaggtcg cgcggcgagg tgcgcgtgcg cttcacggcg aaccagacgt ctccgcgccg
      601 cgaggtcgcc caggcgccgg ttccggtccc gagcacgtgc gcgccggacg gctgcgctcc
      661 acctgcgtcg cccccgcccg ccgcgatcgc ccactcgagc gccatcagcg ttagtccgga
      721 gtacgacgcc gcggcgacgt actcgtccac gcccgggatc gccgcgtcga tgccctgcgc
      781 catcgagggg gtcacgagga agccctcggg cccggtcggg taggcgtcga tgagccggcc
      841 gacggtgcgc tccgcgagcc cgtgctgggc gctgcgctcg gcgccgtagg ccgtgagcgt
      901 gagcgtccac gcctgcgcct gcgagcggcc gtggtaggcg acctcgccgt cggggccggc
      961 catcgcgagc gatgcggcta ccccgcgccg caggagggtg cgcgccgcgg acggcgcctc
     1021 gtcgcccagc agctcgatcg tgcgcgcgag catcccgatc gacagcccgt ggtaggcggg
     1081 cggcagcagc ggcatatcgc ccagcaccgt ccgcccgtcg atctggaacg gccgcgccgc
     1141 ctcgggcagg tcgctcgcga ggaagtgctt cacgagcgcc agcgtggccg cgggatcggc
     1201 gagcgcggcg ccgggctggg tcgaggtcag gccggagcgg accagctcga gcagcaggat
     1261 cgcctcgacg atggacttgt tcgtcacctt ctggcgcccg atccggtaga cctcgatccg
     1321 ccgcagcacg tcctcccagc gcgagcgcgc gcgctggaac accgggtgat ccgcgtaccg
     1381 ctgccgcgcc aggttgtagg ccgagacggt ggcgagctgg tggaagacct gggtcgaggg
     1441 gctgcgcgcg gcgcgctcga gcgagtactc gagggcgcgc aggccggcat cggccagcgc
     1501 gggatcgttg cggcgcgtgg cgttcagcag cagcccgtag ccgaggatcg cgtcgccgta
     1561 gtcgtcgcgc tgatcgctcg gatcgcgcac gagcacgtag tcgcggaagc tcccgctggc
     1621 ctcctggagc cccggccacg gggcggcgat ctcgtccgcc ttctgagtcc aggccggggt
     1681 cggggccgcc gaagctggcg ccgcgagcac catgaatagc gctgcaacgg cgacggctgc
     1741 gtgtttactc acgccgatgc gccgcgccac gctcgtcccg atcctcctcg ccgtgctcgc
     1801 gttcgcgccc gcggcggcgg ccaaggcacc ctccacatgg gggccgctgg ccgaggagat
     1861 cgcggccccc tggcccggct tgcagaacga gaacggcacc taccaagact acgtatacgg
     1921 gggcgacgtt tccttctgtc tcgtgcgcag ctgcaagccc ggcctcggca acgcgcgcta
     1981 cggcgaggcg gtgctgggct acgcgctgct gcgcacgggc gtgcgcaccg gtgacggcgc
     2041 gctgatggac acggctgtcc gctcgctgac atgggtcgcc gagcacccgg agtaccgcga
     2101 cacgctccag accaacttcg agacctgggc cctggccagc gcctacaacc tcgcgcgcac
     2161 ccgcatcgct tcccggccgc tgttccgcca gaaccgcgcg agctgggagc gctggctgaa
     2221 ggaggtaaag ccgcttctgc tgcgcgcggg ctcgcggcgc tacttcaacc accacctggt
     2281 cgaggcggtg gcgacctacg agctgaagcg cacgcgactg cggtcgaagg cccggggcgc
     2341 ggtgctcaac ccggacgtct acaagggggt ggagcggcgg gccgaccaga ccgtgaacga
     2401 ggaggtgcac ctcgtggcgc gcccgacgat gctgcgctcc ggcggcgcgg tggcggcgct
     2461 gctgagcgac cggcccgagt acccgctcgc ctaccagggg ctgtcgatgg cgttctacgc
     2521 ccgcgcgctc gagctgatgg gaagccgcgc gaccgagcgc tcgcgcgagc tcctgcggcg
     2581 cctggcgcac gcctcgtggc tgctcaccgc gccggacggt gacctggcct acgcggggcg
     2641 ctcgcaggag gagtcgtggg cgctgccgat gaccgcctac ggcgtcgagg tggcggcgcg
     2701 gctgccgggc gtgggcgccg gcagcgccgc gcgctggcgg gcgctggccg accggacggt
     2761 ggagcggctg cgcacggcgt atccggtcgg gccgcagggg atgtggatca tcccggccct
     2821 ggcgcgcgat ccgcagcgcg ggctccccgg agtcgacccc tatgccggcg ccgcgatctt
     2881 caacgggctc accctgatct acctcgaggc ggcgcgcgac gaggccgagc gggccaggcg
     2941 gggcgtcggg cgcatcgcgt cggacgccgg cggcccacgc cggctcggcc gcgccgacga
     3001 cacgttcgtg accctccgcc gcggcggcgt ctggttcgcg atccgccagt cacgcgacct
     3061 ccagcgccgc ctcgacgacc tgcgcaacga cttcggcctg gtggccctca agcgcctcga
     3121 ggacgggcgc tggcgcgacg tgatcccggt gcgcccgcgc gtggagagcg aggaccgccg
     3181 agcggccgac agcgccgggc cgcggctgcg cggcggcgtg cccacgggcg agcggatcgc
     3241 ggccgactcc gactcggtca cggtcacggg cggctggcgg ctgcccgacg ggacgttcgt
     3301 ccgccgcggc acgaagttcc gcttcgaggc gatcggctgc ggcgtgcgcc agcgcgttcc
     3361 agtggcggcg ggcgacgcga tcgagtacga cgtgctggcc gtgcgcgatc ccgaggggat
     3421 cagcgtcgac ggggcgcgga tcgcggacgg ccagcaggtc gtcaccttca gcgaggctcc
     3481 ggccgaggtg aagatgctgc ccggctactc gtccggatcg caccccgtaa tcacgcgtgt
     3541 gcgggcaact tttcccgccg ccgcgggggc tcgtacgctc gacatcacca cctgcgaccg
     3601 atgagtcggg gccgcgggcg cggtctttaa ctacatgacc acatcgacca cacctcagaa
     3661 gagctcgcgc tgggccgccc tcgtggtcct gtgcacgggg atgctcatga tcgtgctcga
     3721 cgcgacggtc gtgaacgtcg cgctgcccac catccaggac gacctgggct tctcgcagtc
     3781 gagtctcgcc tgggtcgtga acgcctacct gatcgcgttc ggcggcctgc tgctgctggc
     3841 cgggcggctc ggcgacctga tcgggcgccg gcgcgtgttc ctggtcgggc tggcgatatt
     3901 cacgaccgcg tcgctgctct gcgccgtcgc gcagtcgcag ggcgtgctga tcggcgcgcg
     3961 cttcgtgcag ggcatcggcg gcgcgctcgc gtcggccgtg atcctcggca tgatcgtcac
     4021 gctcttcccg gagccgcgcg agcaggccaa ggcgatcggc gtcttcggct tcgtcgcatc
     4081 ggcgggcggg tcgatcggtc tgctggccgg cggcgtcctc accgaggcga tcaactggca
     4141 ctggatcttc ttcatcaacc tgccggtggg cgtggccacc gcgctgctgg cgcggcggtt
     4201 cgtcgaggcc gacgagggga tcggcttccg ccagggcgcc gacgtgcccg gcgccgtact
     4261 cgtcaccgtc gcgctgatgc tcggcgtcta cacgatcctc gaggtcagcg accgcggctg
     4321 ggtgtcgctg cacacgctcg ggctgggagg catctcggcg gcgctggtgg ccgtgttcgt
     4381 cgcccgccag gcgcgcatcc cgaacccgct gatgccgctg cggctgttcc gctcgcgcaa
     4441 cgtcgtgacc gccaacaccc ttcagatcct gctcgtggcg ggcatgttcg gcatgttctt
     4501 cctcggcgcg ctctacctcc agcgcgtgct gggctacgac ccgctcgagg tcggcctcgc
     4561 gttcgtgccg gtcacgctcg tgatgggcac gctctcgctg cgcttctccg agcgcgcggc
     4621 gatgcgcttc ggccctcgca acacgatcct cccgagcctc gtgctgatcg cggcgggcct
     4681 ggcgctgttc gcgcgcgcgc cggtcgaggg cacctacctg accgacgtcc tgccgccgat
     4741 gctcctgatc ggcgccggcg gcggcctcgg cttcccggcg ctgatgatgc tcgccatgtc
     4801 cggcgtcaat ccggaggacg cgggcctagc gtcggggctc gtgaacacct cgatgcaggt
     4861 gggcggcgcg atcggtctgg cggtgctcgc cacgctcgcc gccacgcaca ccgacagcct
     4921 gcgcgcggat ggagagtcca cggccgcggc gctcaccggc ggctaccagg tggcgttcgt
     4981 cgtgggcgcc ggcctcgcgg tcgcggcgct ggcgctggcg ctcgtcgtcc tgcgcccgga
     5041 gcccgctggg gccgcggccg gggccgacgc ggagcgcgag ggtcagtcga cctccgagcc
     5101 cgcggccgag gcagcctgat ggctgcggct gtcgccttcc cgaacgagtc ggcggaatac
     5161 cgggcggcgc gcgagcgcct gctcgccgcg gaggtcgagg agcgccgtgc gcacgaggcg
     5221 ctcgccgcgg cccggcgtga gctgccgccc ggcggcgagg tgcccgagga ctacgtgttc
     5281 gagcgggccg gtgcggacgg cacgccggag ccggtccgca tgtcggagct gttcgcgcgc
     5341 ggggacacgc tcgcggtcta cagcttcatg ttcgggccgg atcgcgacgc gccgtgcatg
     5401 ggctgcacct cgatgctcga cgggatcgac ggcgccacca ggcacatcgt cgagcggatc
     5461 aacttcgccg tggtcgcgga gtcgccggtg ccgcgcctgc tcgagttcgc cgagcgccgc
     5521 ggctggtcat ggctgccact gctgtcgacg gccggcaaca gctacaaccg cgactaccac
     5581 ggcaaggacc ccgacgggta cgaccagccg atgctgaacg tcttccagcg caccggcgcg
     5641 acgatccggc acttctgggg ctcggagctc ctctaccagc ccatggagcc cgggcaggag
     5701 atgcgccacc tcgactcgat cgacctcttc tggaacatgt tcgacttcac gcccggcggg
     5761 cgtggtgagg actcgccacc ggacctcgcg ccggcctccg ccggctagtc gagcgggttg
     5821 aagacgagcc cggtcgggat cttcgggtag aagaaggtcg acttgggcgg catcgactca
     5881 cccgcctcgg cgacctcctg gacgagctcg accggggtcg cgcccatgaa gaaggcggcc
     5941 tcggcggcgc cgctctccac ggcctcgatc gcgtggtcgg cgcgcttggc gtagccgatg
     6001 ccgttctgcc gcgcgatgtc gtcctctgtg aggccgagtg tgtccatcag gatcgcctgc
     6061 tcgaggaacg cggtgtcgag ccgccggtag ggcggcggct tgctcgcgag cgcacggtcg
     6121 gcgatcgcct ggtcgcgcag tcgcagccgc agcggccgct tgtggtggcc gtcgtagtag
     6181 ccgagctcga tccggtcgcc ggcggggggc tcgaggtcgt cgcgctcgat cggctcgacc
     6241 tcgaagtcgc gctcgacggc ggcgcgcagc gcctcgcgct tttcggggtc gatgcgcgtg
     6301 agcaggcggt gggtggggag cacggtcagc ccggggtcgg acatcgcgca gagggccatc
     6361 aggcagtagc ggtgctcgcc ctcgccgccg atctcctcgg cgtacacgcg cgccgtctcg
     6421 tagcggtggt ggccgtcggc gatcaggagc tcgcggtcgg cgagggcgtc gcgcacggcc
     6481 gcgagctgct cctcgtcacc gcagcgccag acgcgcgtgg tggcgccgct gccgtcggtg
     6541 taggagcccc acgttgcgcc gaggtcgccg aacgagacgg ggtcgccggc gtagaggctg
     6601 aagatggggg agaggttcgc gcggcaggcg cgcgtgagcc gcaggcggtc ctccttgggc
     6661 ccgggctgcg tgcgctcgtg cgggcggatc cggccggggc cgtagtcctc gacgcgcacc
     6721 ctgcagagga agccgccgcg cacgtggctc tgcccgtagg ggtcgacgaa ctcctgctcg
     6781 atcggccaga tcgccggctc gcgctccttg acgatcacgc cctgctggcg ccatgcctcg
     6841 aacgtggtct cggcgtggag gtaggggtcg ccgccgttgc cctgcgggag gtcgatctcg
     6901 accacgttga acgggctctt ggccaccagc tccgcgcgca gcgcgtcgtc gatcacgtcg
     6961 tacggcgggg ccgcgacggc gtcgagcgac ccgacggagt tcaggtcgta gcggagcgag
     7021 cggaggggct ggacgtcggc catcggcggc gggcaggcta gcgagcccgc cgcgccaagt
     7081 gccgatcagg cttggatccg gggtggatca gccgcgcgtg ccgaagtcgg tcgcgtaggt
     7141 cgcgccgtcc tcgtacgagc ggttcggggt cccgcgggag atgccgatgc cgatctcgcg
     7201 gaagccgcgc tgaaggatgt tcgcgcggtg acccgggctg tgcatccagg cgtcgacgat
     7261 ctcgaccggc gtggacagcg tgcccgagcc ccaggcgatg ttctcgccga ggcgccagcc
     7321 gcgcgcgccg cgcaggtagt tcgcgccgcg gatgcggccg acgaagttgc cgtgctcgaa
     7381 gaagttgcgg gcggccatgg agcgggcgtg ccggaccgag gccaggtcga ggcgcgggtt
     7441 gcggcggaac ctgcgaatgc cgcgcgtggt gcgctcgcgg ttgatgaggc agaaggtcgc
     7501 gcgctcggcg acgtccagcg agacggccgt cggctcttcg ttctcgccgg ggcaggcgga
     7561 cgcggtggcg gggaccaaca gggcgaggat cgcggccacg aggacggcga tgcgggcatg
     7621 gcgaagtgag aggttcatgg cggcggggga ggggttggcg gcccccatcc atgagggccg
     7681 gatttccgat ctgacccctc aatcggccgt gtgcggcccc agctgaaccc ccgtccaggt
     7741 ggtctggaca ccctctggac gcaaccgcgg ccggcggctc tagctcgcgt acagttcgcg
     7801 gggcctcggg gcgtagctca gcctggtaga gcactgcgtt tgggacgcag cggccgtcgg
     7861 ttcaaatccg gccgccccga tcagccggaa gccggcgaga actccaccgt ccgcgtggcg
     7921 gggtcggcgc gctccagccg caccgtcagc tcggtcccca cggacgggca gtcgccgtcg
     7981 agcttgccac gcaccgccgg gtcgcgcagc tgcacggtcc cctcgtcgat cacgaccgcc
     8041 tcgaagcgct cgccctcgcg gccctgcagc agcagcgcct ccaccacgtc caccacggcg
     8101 cgctccaccg cccgcgcccg cttgtccgcg gcggccatcg cgcccggcag ctcgggcagc
     8161 ccggcgcgca cccagtccgg caccggcccg ccggccgccg cggcgaggca gcactccgac
     8221 acgtagcggt cctgcagtcg ccgcagcggc gccgtcgcgt gcgcgtacgg cgcggcgatc
     8281 gcgaagtgct cgggctcctc cggcggcgct ccgtcgaagg ccgtgtagcc ggccccgtgg
     8341 cccacgcccg tcgcctcgtg catgaccgcg gcgtgggcgg gcacggcggg gtcgagcgtc
     8401 cgcacgaact ccgggtacga gagcccggcc ggccactcga cccgcagcgc ccgtgcctgc
     8461 tcgcgcagcc gctcgaccgc acgggcctcc ggctcgggct gcgtgcggag gatcccgacc
     8521 cccgcgcgca gcatcaggtc cgccgcggcc atcccggtga gcagcgacac ctgcgcgttg
     8581 tgctcctcgc tgggcagcgg gacgcggtag cggagcgtcc acgagtcgcc ctcgcgctcg
     8641 acctcctgct cgggcacggc caggcgcacc ccgccgcgct cgcgctcgat cgcgagccgc
     8701 cgctcgccca cctcgcgcag cagcggctcg agctccggcg gcacgtcctc gtaggtgtgc
     8761 tgagcgacgc tgcgcaccag ccgccgcgcc accgccgccg cgacgagctc tccggtggcg
     8821 tcgaggtcga gcgtccacag caccgcgggc cgccactcgc cgggcagcag gctggcggct
     8881 ccctccgaca gcaccggcgg gtgcagcggc accttcgcgt ccggcgcgta caccgtcacg
     8941 ccgcgctcgt gcacatcgcg gtcgagcgcg ccgcccggcc ccacgaacgc ggccacgtcc
     9001 gcgatcgcgt agctcacccg gtgccccgcg ccgcggcgct cgatgtgcag cgcctggtcg
     9061 aggtcgcgcg agcccggcgg gtcgatcgtc acgaacggga gctcggagcg ctccccagcg
     9121 acggggactc gcgccgccgc ctcggcctcc gcctcgaccg ccggggggaa tgccgcagtc
     9181 acgtccgcct cgcggcggat cgcctcgaac agcggcgccg cggcgtcagc gggggagagc
     9241 cggatcggac ggcgggtcat cgccagcagg ctacgcgcgt acgacgaggc cgaacgggta
     9301 gtgagcccgc atgacgacca aagcagactt caacgcggac gagtggtcca cgatcaccga
     9361 ggcgcccatg ctcgcggcgc tgcgcacggt ggccgccggc cgcggcggga cgatccgcga
     9421 gagcatcgcg gtcgggaagg tgtacgccga ggccaagcag gggcaggaga cgaacccgct
     9481 cgtggccgag ctggtcggct ctccgccaag cctcgatccg cagaaggtgc agagcgaggg
     9541 cggcttcgag gccgtcagcg acgcgcggct ccgcgaggcg ctccggctgc tgggcgagaa
     9601 ggccaccccc gacgaggtcg aggcctacaa gggcttcgtc atgagcctgg cccgcgccgc
     9661 ggccgaggcg cacaaggagg gcggcttcat cggcatcggt ggcaaggcga tcagcgccga
     9721 ggagcaggcc gcgctcgacc agatcgagtc gctcctgcag ggctcctagc cgctcgagcc
     9781 cggccatccg cgcagggcgg gcgcgcaggg ctcccccgcg cgcccgcctg cctcggagtc
     9841 gctagcggtc gcggatggtg atgtgaccgg aggtgatcgg agccggtccc gactggtcgg
     9901 cgtcacacag ctcggtggtc ggcggttcgc cgaggaacag gaacaccgcg agccggtcgc
     9961 tctcgctgcg gttcttgccg ttgtcctccg cgaacaggag gaacgcgtcg ccctcctcca
    10021 cgttcggcgc accgtaggga gccggcccgg agatcgaggt gatccgaccg gctatcaccg
    10081 cgcgctgcgg accttcggtc tggaggcaga tcacctcgcc gcggacctga gttccggtcg
    10141 aggggttctt gaggcgcagc gtgcccttcg cgctggtcgt gtcgccgatc gcgttgaacg
    10201 cgaagcggaa gtcagttcca ctcgcgttcg ggtcgggcgt ggtgccattg ccctcggcga
    10261 ggtccttgcc gccgccggcg ttggccggcc ccaccaccgc tgcgaaggcg atggcgagga
    10321 tcgttgtcag gactgtgagc cgtccttggc gtgagagtgt cattgcgcca gcgtatgcgc
    10381 accggtgtag acctgtcaat cggcaacgga gagcccgcgg cggcgcgtct gtagcatccc
    10441 ggaccggagc agcgggtgag gacgagtaca gcgggaaatg cgggcatgcg gcgccttggg
    10501 gcggcagctt cggtcgccct cgtggccgcg gcgccgtacg tgatcttccg cagcaccttc
    10561 gaggacgccg cgaccgactt ccgctgggag ctcgtctacc tgatcagcgg ctggggcccg
    10621 ttcgtactga tggggatcgg ggtgctgtgg ttcgtgccgg tggtcctctc gatgcaccgc
    10681 acgggctact cgcgtctcta cctgcggccg gtcacccgcc acgcctgtga ggcgtggggc
    10741 ctggtcacgt acacgctcgg cttcatgctc gcgatccaga cctcgcagat cgccgggctg
    10801 ttctagcccg accgcgcccg cctgatcgcg tcgaacgcgt agtacaggcc gaaggcgtat
    10861 tcgccgcggt cgtcccaggt cgagccgccg ccgagcgagt agacctggtc gccgagcatg
    10921 ccgatcgggg gtagccggct cacgcggccg agcgatgcgg acagcatccg cagcccgagc
    10981 cggcgccagc gcgggccgtc ctcgcacgcg cgcgcgatcg actcgcacgc cgcggcgagg
    11041 cgcagggcgc ccgcggccgt gatcgtcccg gcggaggtgt cggtcggggc gggggcgggg
    11101 gcgtcgtagt cgtacggggg gatgccggac gccggcaggt gccccgccac gtacccggcc
    11161 acccgctcgg ccgccgcgag cagcccgcgg tcgcggaagc gcgcgcccgt gtcggcgaag
    11221 ccgtagagcg cccagccctg gccccgcgac cacgtgctcg tgtccgagta gccctgccgc
    11281 gtgccgaccg agacgacgtg cccgtccgcg cgcgaggcga tcaccgactg ggcggtcgag
    11341 ccgtccgggc gcacgagcag tcgctcgacc gcccgcgcgt gacgcagtgc cacctcgcgg
    11401 tagcgcgcgt cgccgcgctc gcgccaggcc cagccgagca ggccgaggtt catcaggctg
    11461 tcgatgatcg tctcgctctc gtccgccgac gcgcagcgac ggcagtcgcc ctcggacccg
    11521 accgtcggga tggtgccgac cgcctcgttc ccggcggcca gctcgagcag tgtgttcgcg
    11581 gcggtcaccg cgctcgcgcg cagccgcctg cacgccgacg acgagcgcgc gcgcccgccg
    11641 cactcgcggt cgtgcgcggc ggctgaagag agcaggtagc ggaagccttg gtcgtggatc
    11701 gcgtcgcgct cgcggccgcg gtggcggtcg gtggccgcgc gcgccaagtc ggcgaacgga
    11761 gcgcttggcg cggtcatgtc cgcggcgagc cagagcatcc cgggccagaa cccgtcggtc
    11821 cagtcgcgcg tgaagcgcag gagcgagtcg tcgtccccga tcccgaccgg gaagcgcccg
    11881 aggggcgtgc gcgcgtgcag ctccgcgagg cggtggagga gcgcggcgcg gcggtcgtcg
    11941 cggcgggtgg ccacgaggcc ggtcgcctgc gccgagccct cgaccacgcg cacgcgcacg
    12001 gtggagccgg tcgccgccag ctcgttctcg atcggctggc cgtcgatcgc gagcagcggg
    12061 tcgctgccgc cggcgatctc gaggtgacgc cagtcgcggc cggcgtagcc ctgctcgggg
    12121 agcgcggtcg gagcgatgcc ggggccgcgc acgcgcgtcg tgccgtcgcc gttgcggtcg
    12181 aagacgagct tgtcggcccc gagctcgacc accacggtgc tgtggtccga gggccggagg
    12241 tcgaccgata ccgaccacgg ccggcgcggc agcgaacgcg tgaccgaacc gcgggcgacc
    12301 atccgcgcgc cggagccgtc gggcaggccg atcgcccgca cgaggttcgt gccgcgggcg
    12361 ggcgcgagat cgtcggccac cacgtcgggg agcggggcgg caccggccgg ggccgccgcg
    12421 acgagaatcg cgaacgcggt cgcgaggagg gcgcgcttca cagggccgag gctagagatg
    12481 ggaccgggca cagccctctg aacacccgaa cgcggaacta gactcgccgt cccggccccc
    12541 gtagctcagt ggacagagcg ggcgacttct aatcgctagg ccgctggttc gaatccagcc
    12601 gggggcgcta gaggatcgga ccgccggacc cgggcacctc gaccgccagg catgggtcgg
    12661 gaagctcctt ttcctggtcc gacggctcgg gatcgaacag gaccgacgtc atgttgtcgg
    12721 cgaagctggc gccgcccctt ctcaggatga acagccgctg cgaccgcggg tcgttcgggt
    12781 cctcctcggg gaacgtgggc ggtggcttgc cgtcggacag gtcgctgatg atcgtcacca
    12841 tctcggggcc ggtgcggacg tggaaccccc attcgccgtg ctcggtcgtg aagtcctcgt
    12901 cgtcgaagca gcccacgcgc gtgccgcagg ggcccatgag gatcagcgcg ctgacccggt
    12961 cgttcggccg caggtccttc tccagccagg tcgacccgag gaacttgtcc acgcgcatgt
    13021 ggatgtcctc gaggttgtcc tcgctcccgc gcttgacccg gaccagcggc tggctggcca
    13081 gcgcctgctt gagagtgtcg atgccggtga ggaaggcggc gatccccacc agcgaccgca
    13141 gcggccggag tgcttcgtgg atccggtcca gcagatcgtt gagcggctcg ctgatcccct
    13201 ggaggatcga atggtgcggg atcagcatga ggacgaaacc gggcgtgtcg ggatcctcga
    13261 acgccgcgaa gttcttgtgc cggaacagcg cgatgaactt cgggatctcg agctgcccga
    13321 gctcgaccgg gaccgacagg ctcttcgtgt cgctcgccag accgccggcc ctcaccgtga
    13381 ccgacgcggt gatcgtccac gacaccgtcg gcacgggcga gcgcgtgagc tcgactatcg
    13441 gcggcttgaa gacgaagccg acgagcagcc cggcgagccc gttgttggcg acgaagtccc
    13501 ggccggggat tgtcctccgt ttgggggaca gtggggaatt agcccggcag ctgaatctcg
    13561 tcggcgaact cacgcaggag ctcggggcca ggcgccgtga acaggcgctc gacgaactgc
    13621 tcccagcgca gcgccggccg catggttatc cgcaggcggg tctcacctcc gctcaggttc
    13681 cacgcgctgt gcaccacgcc gcgcggggcc accacgcgtt ccccgggacc ggcctcgagc
    13741 tcgtcgtcgc cgatccggaa gccggcgcgc cccgacacga cctcccacgt ctcctccatc
    13801 cgcggatgta ggtgcggcgc cgtcgcatga tccgggcgca cccacacgtc ctccatctcg
    13861 agcaggtccc cgccggtgtc cgcggcggtg cgcaggaacg tgatccgctc ccccgtggcg
    13921 gggttctcca gcgtgtctcc ggcacccggc atgcgcgcta cagtctggcg caggtgccga
    13981 cgtcccttgt caccggcgga gcgggcttcc tcggctccca cctctgcgac tacctcatcg
    14041 agcgtggcca ccgcgtgctg tgcgtggaca acctcgagac cgggtcgctc cagaacatcg
    14101 cgcacctgcg cgggcccgag ttcgagttcc tgcatctcga catcacgcac cactacgagg
    14161 tcgccgagcc ggt
//
LOCUS       DASVPL010000265        38136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_267317, whole genome shotgun sequence.
ACCESSION   DASVPL010000265 DASVPL010000000
VERSION     DASVPL010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 38136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38136
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267317"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VF517_14625"
     CDS             complement(<1..1375)
                     /locus_tag="VF517_14625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX8054222.1"
                     /translation="MEHLEVFIVALFVAVAGLNALARWLTIPYPIPLVVGGLALGFVP
                     GMPEIELDPELVLTIFLPPLLYSAAFFSDLRALREDVRAISMLAIGLVLATIGMVAVV
                     GHEVIGLSWPMAFALGAIVSPTDPVAATTIMRRLGAPRRVVNIVEGESLVNDATALVA
                     YRVAVGAAVGGSFSAADAGLEFVGAAAGGIAVGLAVGFVIGEIRRRLDDPPSENTISL
                     LTAYAAFIPAEQLHASGVLAAVTCGIYMGWRAHTFASPETRLQGFAVWEILTFLLNAA
                     LFVLIGLQLHVILGDLDGRGAGELIGYAALVCAVVIGSRFAWLFTVPYLIRAVDRRPA
                     QQQRRVGPRPRIVTAWAGLRGAVSMAAALALPLETDAGAALPDRDLILFITFALIVVT
                     VVGQGLTLPVLIRRLGIRQDGSEEEAEEIRARVTAAKAALERLDALEAEEWVREVTLV
                     RARRLHQF"
     gene            complement(1406..2893)
                     /locus_tag="VF517_14630"
     CDS             complement(1406..2893)
                     /locus_tag="VF517_14630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8054223.1"
                     /translation="MRQLTSLDSQFLALESARQTGHVAGLAMLDPSTKPTGSLGCGDI
                     QDLISERLPLLPPLRWRLAEIPLGLDYPYWIDDADFDLDFHVRELALPPPGDDRQLAE
                     QVARIVSRPLDRARPLWELYLIHGHESGHVAMLTKIHHAVIDGMSGAEIMGLLLDLSP
                     EGRELPEPPPAQHERKPSDLELLARGVLGVPRYPMRLARSLPTAIPNIEETPLANLPG
                     AGTVGRLGGLARRLVSGDGQIPDRRRLVPPRTSFNGRISPHRRFAFGQLSLDDVKSVK
                     NAHGSTVNDVIVSICAGALRRWLIEHDELTNDPLVAQIPVSVRTDEQVGTYGNRIMLM
                     SAPLHTEVADPVKRLLRTHESLSEMKERHRALPAELLQDANHFIPPAVFSRAAQLTFR
                     LSTNAQIGRPTWNLVVSNVPGPQFPLYCAGARLDANYPVSVITDGMGLNITVMSYCGH
                     LDFGIVADRDQMKDVWCLLDWLGDELALLRPSRTGPPAAEPVPQV"
     gene            complement(2971..4269)
                     /locus_tag="VF517_14635"
     CDS             complement(2971..4269)
                     /locus_tag="VF517_14635"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8054224.1"
                     /translation="MTSSNGSPSPRRAAVIGGNRIPFARSNGPYADASNQDMLTAALD
                     GLVARFALQGERLGEVAAGAVLKHSRDFNLTRECVLGSALDHTTPAYDVQQACDTSLE
                     ATALVANKIALGQIESGIAGGVDTTSDAPVEINDDLRRVLMDMNAEKSTAGRVKLLGR
                     LRPGQIVPAIPRNQEPRTGLSMGEHAAVTARQWEVPRLDQDELAAASHRNLAAAYDRG
                     FMHDLVTPYLGLERDQNMRPDSSAEKLASLRPVFGTAEGGATMTAGNSTPLSDGAAAV
                     LLASDEWAAERRLPVLAHFVDVETHAVDYVHGDDGLLMAPLYAVPRLLDRHGLTLQDF
                     DYYEIHEAFASQVLATLKAWEDPGFCRERLGRGAALGPIDRSRLNVAGSSLAAGHPFA
                     ATGARIVATLAKLLAEKGSGRGLISICAAGGQGVVAILER"
     gene            4311..5717
                     /locus_tag="VF517_14640"
     CDS             4311..5717
                     /locus_tag="VF517_14640"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF006110.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX8054225.1"
                     /translation="MADRYTTFANSGPGSFVTRQLGLPRPAQLRRHQPGDPVVDGAVL
                     LGGALGGRLAVPAAAVTRSIGATTHVPGAAHLREAATAAGPETAAFDPAVDEARRFAA
                     IVYDASGVEDTDSLAQVYDALHPVIRRLQPSGRLVVLATPPENAETPLHAVAQRALEG
                     FVRSAGKEVGANGSTANLVYVAPGGEGGMESTLRFLLSGRSAYVSGQPIRIGAGDAPA
                     PADWERPLDGKVALVTGASRGIGEAIAEVLARDGAHVVALDVPAQGEALAAVANRIEG
                     SSLQLDITAPEAPRRLADHLRERHGGVDVVVHNAGVTRDKTLGRMSADQWNLVLDINL
                     SSQVRINDVLLKDDVLRPGGRIVTVSSVSGIAGNRGQVNYATSKAGVIGLVDALAPVV
                     AGRPATINAVAPGFIETQMTAAMPVATREGGRRLNSLAQGGLPVDVAEAIAWLAAPGS
                     GGVNGNAVRVCGQALIGA"
     gene            5714..6595
                     /locus_tag="VF517_14645"
     CDS             5714..6595
                     /locus_tag="VF517_14645"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX8054226.1"
                     /translation="MTEVRELESQPRMRVLYPKAAAGAARGLLQRLPGVGGGEPVLPD
                     RELRLGGIEVERDHLAEYDRVCGFRLRDVLPPTYPHLLAFPLSMELMTAGDFPFPVIG
                     MVHVRNRIEQLRPIEARERLDVRVWADGLRPHDKGTQFEMRAEVAAAGEPVWRSASTY
                     LRRSGGDSGTKDGGGERPDPPRAQAKWKLAGGVGRDYAGVSGDSNPIHLHPLTARAFG
                     MPRPIAHGMWLKARCLAALDGHLPDALDVDVAFKLPVYIPGTVSFASWLDGSGRGFAL
                     HDAKNEKPHLTGSAQPA"
     gene            complement(6495..7667)
                     /locus_tag="VF517_14650"
     CDS             complement(6495..7667)
                     /locus_tag="VF517_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX8054227.1"
                     /translation="MTGRRRDIDWLRAGAVYLLLLFHSARPFDSGSWHIKDSDRPQAV
                     ETLTGAISAWHMPLLFTLAGWSIVPSLDRRGADGLLRERRQRILVPFAFGTVALIPVC
                     AWVEQRHKGNTEESFLEYLPSFFTSLDQFTWMHLWFLIYLYVFTTLYLPAMRRVHARG
                     WQVERVPPWMIYAAIVPLAAVQVGLRGRWPGYQNLYDDWANFAFYSLFFVGGFLLTCF
                     PEVERTLHGERRRAAVLGISALIGMALLAGNGMPATGSAAWIALHSLSAVAALCIVVA
                     ALGYGAQLLAGSERGLDFARNSAMPVYVLHQPIIILLALPIVGLPAGVPLKLGLLVGA
                     STAATLLAYRLAQRSSTLLWLLGSKPAVRTRSGAASRSSRRGARIRGLTRRATTRS"
     gene            7697..8323
                     /locus_tag="VF517_14655"
     CDS             7697..8323
                     /locus_tag="VF517_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054228.1"
                     /translation="MTTATRESTRDRLITAAMHLFADRGYARTTVGDIESAAGLAPRS
                     GALYQYFKGKRELLDAALERHVEDLDQIQGAIDLLPLGDLRAELTLMARWNLQDLLRR
                     RDLHRFLRKEGDQFPDLVAKVYERMGAGPLRRTAEFLSGRLRDAGAEPIDAEAVVVVM
                     VQTMAAYRTHEDIYGAPPLDVDEERFVQAWVDISLAYARSRGVDVASS"
     gene            complement(8320..9228)
                     /locus_tag="VF517_14660"
     CDS             complement(8320..9228)
                     /locus_tag="VF517_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8054229.1"
                     /translation="MNEEFANVNGVELCYEHFGDPGDPPVLLIMGLGTQMIAWREDFC
                     TQLAERGFFVTRYDNRDMGRSTRFDHVPPPRTQELLTRRPRQLAYTLADMADDAAGLI
                     EELGFESAHIVGASMGGMIGQILAINHPERVRSFTSIMSTTGSRFVGQPAAAVYPFFM
                     KPIPKSKDAYIERGLQLWNVIGSPGFERDEEELRSVLSRTYERGLSPAGTMRQLAAIA
                     AAGNRANDLKRITAPTLVIHGTKDKMIRVSGGKATARAIPGARLELIDGMGHDLPRGV
                     WPRVIDGIEQNAARAADAGERQPAAA"
     gene            9436..10383
                     /locus_tag="VF517_14665"
     CDS             9436..10383
                     /locus_tag="VF517_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8054230.1"
                     /translation="MRGIRIEEFGGPEVLLPAELPDPEAPEGGVVIEVSAAGVNWADT
                     HQAENSYLAPAQLPLVPGGEVVGTAPDGRRVVALVPAGGYAEKAVAFEAAMWEVPDGV
                     SDGQALALVLQGTTAWHLLRTSAQLRPGESVVVHAAAGGVGTIAVQLAKRFGAGRVIG
                     LASSEEKRELATRLGADAVVDSGAEDLTAALREANGGERVDVVLEMVGGRAFEQSLEA
                     LAPFGRLVHFGQAGREGAPAVDPGRLMGTSRGVLGFWLVHMMRRPDLMSEAMEGMFEM
                     VIGGELEPIVGGEYPLAEARRAHEDIRSRGTVGKLVLTP"
     gene            10380..11420
                     /locus_tag="VF517_14670"
     CDS             10380..11420
                     /locus_tag="VF517_14670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX8054231.1"
                     /translation="MSGDLKLGYNTGYWAGGPPPGMQDTILEAERLGYDSIWTAEAYG
                     SDCLTPLAWWGSRTERLRLGTAIVQMSARQPAATAMAAMTMDHLSGGRFALGLGVSGP
                     QVVEGWYGQSFAKPLARTREYIGILRDIWARQGPVTNDGVHYPLPLPGGTGLGKPLKA
                     GIHPLREDIPVYLGAEGPKNVALCAELCDGWLAMLLCPDNQDQFESYLDEGFAREGAR
                     RTKEEFDVAATVPLIVCDDVQDGIDGMRPFYALYFGGMGAKGANFHANVPARMGYEDE
                     VREIQELYLGGKKQEAAAAIPAELIEKMSLVGPLDKIRHDLDAWRESIVTTMLIQGDA
                     NTLRTAAELVLG"
     gene            11420..12487
                     /locus_tag="VF517_14675"
     CDS             11420..12487
                     /locus_tag="VF517_14675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX8054232.1"
                     /translation="MRRILLAGLVAVLALPATASAADPVTISWVGDMSFSRGHGLPRS
                     PDSVFAPLRSSLAADIVTGNLEGTLGRGGPSKCGGGSTNCHAFQAPASYAGVFRRAGF
                     EMLNLANNHSRDFGSSGLAQTRSALHGARIAHTGLRGQVRVLEANGVRVAFLGYAPYP
                     WTGPLLDVGKGRSEVRAARVKAARVRLRARREVAAAASRADVVVVFMHAGAEGSGATH
                     VPHGRETAFGENRGETRRFARTMIDAGADAVLGSGPHVLRGIECRRGRPIAYSLGNFV
                     GYHTLSTSGVLALSGVVRLRIGPDGRFLGGRLVPVELQGPGVPRLDPKRRGVALVRRL
                     SRQDFGSSRCRIEADGKFRAP"
     gene            complement(12501..14699)
                     /locus_tag="VF517_14680"
     CDS             complement(12501..14699)
                     /locus_tag="VF517_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8054233.1"
                     /translation="MPAEERITFCRICEANCGMVATVEDGRVTKLRPDREHPLSQGYA
                     CPKGIAFPEVQEDPDRVIHPLRRRADGEFERVRWDEALTDIGNRLRAVRDEHGGDSIG
                     MYMGNPAAWSFSHTVWGKGLIDALGSPHYYSAGSQDVNNRFAASALMYGSPLTVPIPD
                     VLRTDLLVMFGANPFVSNGSVLSIPRVRDRLREIERRGGRVVVVDPRRTETAREFDHV
                     PIRPDGDARLLLSLLHTIFEEDLVDHDAVREQARGVGVLARLANGFAPEATEPWTGVP
                     AERVRALARDIATAPSAAIYGRTGSCLGRHGTLTAFLIDALAAVTGNLDRPGGIVFGS
                     SPLDLEGAAERAGLATYGKRRSRIGGFPDVLGQMPATLMAPEIETPGPGQLRALIVTA
                     GNPVQSVPDGAALERALGKLDLLVSIDIYMTETGRRADYVLPATTWLEREDVPVAFQQ
                     FFYRPFIQHTEAMVPARGEARPEWEIFDAFARALGVVASSIAPMRLLGRAGVRIGPRR
                     MLDLMLRNGPRGDRFGLRRGGLSLRRLARAPHGLLLGDEWPTGVLRGRVRHRPALVDL
                     APGEIAGEVARLGAEPAPGEGELPLRLIGMRELRSHNSWMHNVPALMRGERRHALRMH
                     PRDALARGLEDGAPARVRSASGEVSVPVLVTDEMTPGVVALPHGWGHRGGWRVANAAG
                     GANANDLMSAEPESLERLAGMSNLNGVPVEVEAAPAEATPAEAAPTAAAG"
     gene            complement(14689..15570)
                     /locus_tag="VF517_14685"
     CDS             complement(14689..15570)
                     /locus_tag="VF517_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8054234.1"
                     /translation="MSRSPLLGEERSVRLSAGELRYRERGRGRPIVFVHGFLTNADLW
                     RNVVPPLAESHRCIAPDWPLGSHTVAMDPDADLTPHGTARLIAEFLDALELDDVVLVG
                     NDTGGALAQMVATRHPDRLGALVLTPCDAFSNFPAWWSKPLRPIGRHPRLMRIAGLPL
                     RARRVQRLPLVYGWVVKRHPPPEIAAGWVEPGLRDEGVRRDFGKAFSTTHPSQTQEAA
                     RRLPEFGKPALVVWSTERTRIYPLAHGERLAKLLGAPLELVDDSYIYVPEDQPRRVAE
                     LIDDFLAEKARPYAHAR"
     gene            complement(15567..16403)
                     /locus_tag="VF517_14690"
     CDS             complement(15567..16403)
                     /locus_tag="VF517_14690"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8054235.1"
                     /translation="MRSGSEQPKGRAPEGRAEQRRTRVEQQRRTRAELVAAATRVFAT
                     KGFHGASVGDVAADAGYTTGAVYSNFGGKEDLFLAAFEHEVARHAREVTEAVAAAGDD
                     PAARTRAAAGQWTAFLDRAPERFLLFVEYWAWSVRDPERRERFGERFAAFRATTERMI
                     AEAAPGLPVDRLAVIANALTYGIAFQRLAEPDAVREDAFAEGLALVFAGAVARGEGAA
                     GGAAAGMGGAAAGGAAAGSGGAAAGSGGAAAGSGGAAARGEGAEARDEGAAAARGEGA
                     VR"
     gene            complement(16422..17123)
                     /locus_tag="VF517_14695"
     CDS             complement(16422..17123)
                     /locus_tag="VF517_14695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX8054236.1"
                     /translation="MEERAPGSPDEEILSAESPAVATVETDEERLALIHDELVTGFES
                     LSGLGPAVSVWGSARSSRDGAEYELGRAVARSIGERGIAVITGGGPGLMEAANRGARD
                     AGVRSVGLRIELPFEQGMNPYVDLPLHFHYFFTRKLMFVRYASGFVALPGGFGTMDEL
                     FEVVTLIQTGRARDFPVVLVGSDYWSGLVDWLRERVAGDEKIGAEDLDILTVTDDPDE
                     VAEIVWRAACAQGLA"
     gene            17149..17595
                     /locus_tag="VF517_14700"
     CDS             17149..17595
                     /locus_tag="VF517_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8054237.1"
                     /translation="MGEDEKLDVKEEVELLQSALRLQYRSAVQYTLAAGSMVGFAYSH
                     FGTSLWEFAQADLADARRLVEKIKTLGADPTTDVAPLHYAADPTELIGQLIEDETECA
                     EALQASIGPTGREARSEAIEHRLEHMIMRKQEQIDLLERARREPGS"
     gene            17696..18580
                     /locus_tag="VF517_14705"
     CDS             17696..18580
                     /locus_tag="VF517_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8054238.1"
                     /translation="MSTKHVDLSAGRIRYRDTGEGPPMVFVHGLLVDGRLWRKVTPAL
                     ESEFRCIVPDLPLGAHTIPMKPDADVSPPGLARLIAEFLEKLDLEDVTLVANDTGGAL
                     TQIAMTQSPERIGRVVLTPCDAFDNFLPPMFQPLQVLARFQPALTAFVQPLRLRPLRR
                     LPLAFGWLTKRPISDPDVEAAWVGAFFKRGDVRRDVTRILRAIDSRHTQEAAERLGSF
                     DKPVLIAWATEDKVFPLEHAHRLAERLPDARVEEIADSYSFVPEDQPERLAELIRGFA
                     GAGASKGSGAETAAVKGG"
     gene            complement(18670..19977)
                     /locus_tag="VF517_14710"
     CDS             complement(18670..19977)
                     /locus_tag="VF517_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEX8054239.1"
                     /translation="MNAELAAAAGSVLDANWLGSSTLPSPRLYPHQWSWDSAFVAIGN
                     AERASDRARCELDTLFAAQWTNGLLPHIVFNPESEAYFPGPETWRTEVSPHASPGRAT
                     SGIVQPPVHAVAAWRVHELAPEPDLDWLARLFPRLAAWHDYLYRERDPEGDGLVAIRH
                     PWESGMDDSPAWDAPLAAIELEPGAVPPYPRKDLTMARADERPTAAAYDQYVHLVETF
                     KRFRYDEREIRARSAFVVEDPCFNAILVAAGQCLARIAEAVGEDGARFREQAARTAAQ
                     VNTRLWNDALHAYVPFDRVARRQVLAPVAGGFVPLFAGIPSARQAQLMCRQLDSAHFW
                     AHGGAGWPVPTCDRVFPGFVPSRYWRGPVWVNVNWLIARGLRRYRFAAYAEAIEAATL
                     RLVRDGGFKEYFHPVTGEGLGSESFAWSAALVLDLLRTSTAST"
     gene            complement(20138..20764)
                     /locus_tag="VF517_14715"
     CDS             complement(20138..20764)
                     /locus_tag="VF517_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8054240.1"
                     /translation="MEPITAALRDGSVVAIRQIEPDDKERLRAAFHRLSDESRRRRFM
                     GPAEELAPEDLVYLTELDHRRHEALVAVDPESGDILGVARWVRQPGRREVAELAVAVV
                     DDRQGQRMGSELVAAANRRAREEGIERYHAIVSTDNVQVIEALERNGAVRRGAQEDPE
                     AIDFEVDVPGEGVGAGLAAGLRAAASGQLRMVGAAADWVRALVPWSRD"
     gene            complement(20778..22610)
                     /locus_tag="VF517_14720"
     CDS             complement(20778..22610)
                     /locus_tag="VF517_14720"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX8054241.1"
                     /translation="MARLRALEQWLLSLVIRRGEGGLLDPAVLQRALGSLFVAGATLG
                     TVSMAFPQPPGTDVPGLFVLFALAYVGGALLLLGQGRLPAWTPHAGLAFASVLVTLGI
                     NFTDQRTSVYAMFYLWVSITAFYFLPWAAAAVQIALITVLFAVVLAVEQPAAYEEQWI
                     ITMGTVVVAGLFVGTLRRGVERLIGDLEEAHRTDHARLYAAEREARLEADRASQSLRR
                     LQAVTDVALSHLKLDELLEELLNRVTEMLEVDFAAIVLSEDDDSLRLRATRGLTTDLG
                     QLRLRPGEGFVGRVAAERRPIVLEQVHEADVIPPLRGSGMESLMGVPLIVEGRVTGVM
                     PLGSYTRRAFTPEEVRLTQLVADRMAIAIEHARLYEREHRIAETLQRSLLPHVVPSVA
                     GVTVATRYLPAKAEAKVGGDWFDVVELSGGGMAFSIGDVAGHGIEAAALMGQLRNSLR
                     LAALEGEDAGRAMASVDRLLQSQRLREDSIATAVVARLNGDGLTLEMASAGHPPPLIV
                     RPDGSVSFIDHPRSVPLGVPAPGGRETGSCTLEPGSLVLLYTDGLVERRGESISDGLD
                     QLAAAAAAAGHEPEAACDHVIEAMLGGEGPADDVALLAVAVDRR"
     gene            22741..23751
                     /locus_tag="VF517_14725"
     CDS             22741..23751
                     /locus_tag="VF517_14725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8054242.1"
                     /translation="MPRTRPANHLIAAALVLVLALAATACGGSGGGSDDRISLVGYST
                     PKEAYAKLTEAFKQTPEGEGVEFSQSFAASGEQSRAVASGLPADIVALSLQPDVDKLV
                     EPKKVAADWNKDRYEGFVTRSVVVLAVRKGNPKNIRRWEDLTRDGIEVIEPNPFTSGG
                     ARWNVMAAYGSQTAQGKSDAEAQQFLKDLFDNVTVQDKSARDSLQTFGSGKGDVLLAY
                     ENEAIAAQKKGVELDYVIPDQTILIENPIAVTEEGKDNEKATAFLKFLRSEAGQRIFV
                     EEGYRPVLESVNDEKRFPKPKQLFEIGEFGGWSEVNDKFFDPDKGVMAEINKAKGVST
                     GG"
     gene            23744..24592
                     /gene="cysT"
                     /locus_tag="VF517_14730"
     CDS             23744..24592
                     /gene="cysT"
                     /locus_tag="VF517_14730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX8054243.1"
                     /translation="MAEAAAIAATGAPRRRSRRLRVAPGSALGIGLITTYLSLIVLIP
                     LAAVVDRSLEEGVGAFWEAVTAPQAVASLKLTFAVSLVVAAVNAVTGTMIAWVLVRDE
                     FRGKRFVNALIDLPFAMPTVVAGLTLLALYGPKGPVGIDVAYTRAAVALALLFVTLPF
                     VVRAVQPVLMEVDREMEEAATSLGASPWTTFRRVVLPSLRPAIVAGSGLAFARALGEF
                     GSIVLISGNLPYKTEVASVFIFGRIETGDVTTAAAVSVVLLAASVLLLAALSRLGRPR
                     WVDDGR"
     gene            24582..25382
                     /gene="cysW"
                     /locus_tag="VF517_14735"
     CDS             24582..25382
                     /gene="cysW"
                     /locus_tag="VF517_14735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX8054244.1"
                     /translation="MAGRRAIRGVALGYLGLLLAAPIGMIFFRAFEHGLGPFFDALTT
                     PGVTHALWLTLLVVAIAVPANTAFGIGCALLLARRRFPGRTLLDRMIDLPFAISPVVV
                     GLALILVYGQDGWLGGALADQGVRIIFSTPGIVLATIFVSLPFVVREVLPVLREIGDE
                     QEQAAHTLGASRWQAFLRITLPAIRPAVIYGVVLSTARALGEFGAVSVVSGRLEGRTE
                     TLTLYVQNRFEGFDLTAAYATAVLLALLGMGVLVTLTVLSRRRSQHGY"
     gene            25372..26334
                     /locus_tag="VF517_14740"
     CDS             25372..26334
                     /locus_tag="VF517_14740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEX8054245.1"
                     /translation="MGIETAGIHKRFGEHVALEDVSVLVPEGSLTAILGPSGGGKSTL
                     LRVIAGLEQPDAGQVLINGRDVTGDPAQEREIGFVFQHYAAFKHMTVRDNVAFGLRIR
                     KRPKAEIARKVDELLDLVQLGTYVDRYPAQLSGGQRQRMALARALAIEPRVLLLDEPF
                     GALDARVRNELRAWLKRLQAEVGVTTLFVTHDQEEAMDLADQIVLVRDGRVEQAGTPH
                     ELYEKPANDFVMSFVGLVTQLDGKLVRPHDLQLLPTPFAGTSDEAVVERVSRLGFEVR
                     VELTLASGEGVVAQLSRAEERELELREGDIIHVRGVDGAASLAA"
     gene            26414..26872
                     /locus_tag="VF517_14745"
     CDS             26414..26872
                     /locus_tag="VF517_14745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8054246.1"
                     /translation="MRVSAKTDYALRASLELARAEGGHPVKADHIALAQQIPVRFLEN
                     ILGELRHAGLVESRRGADGGYMLARPGAAIALADVVRAVDGPLANVGGRRPDALEYPA
                     GAEALRDVWVAVRAGLREVLERTTLADIAAGQLPDAVAALVEPEDAWQPR"
     gene            complement(26958..28904)
                     /locus_tag="VF517_14750"
     CDS             complement(26958..28904)
                     /locus_tag="VF517_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054247.1"
                     /translation="MRRSRLRRLRIPLSALAIAVAAALGVSANDDGPGGESRAAAGQK
                     PVDPLIDAMRARYPEIQRPNGHFRSSIGGGTRYGNAYMGYALLMSGVRTGDDAAVEAG
                     LRGLTNPLQPGRRPNRPSVFESLALAAGYNIAREHAAGEPLFKRFRAAWEDFLRDAPL
                     IRLPAVTYYGNHWLIEAVEIRELLATGLRSESPTSVLGGQRANADRLSSELINKRIPA
                     MARAEAVAAPNGRAFVLSDPPDNPLAYQGLSLGFYARGVELLGDEASDAARRTLAEVA
                     RASLLLAAPDGDVAYFGRNQEQAWGLAGTVYGAYAAADLDETGSAEDGQLRELASRAL
                     QRLLTVHGVTHWGLEITPSVARSRWVPPAGLDSGAGGPSFGGLVLALLEWARDESREP
                     PDRVSIPADRPLRTKLSHAESRFTVIRRGPVWAVIRPTISGKYPYDVRYDFGVVAMKV
                     LRSDRWVDVLRHRPFTAADPDSAGPILRTGGVPAFPFASDVGVAPNGAVTMRGGWRLA
                     PTRVKRVVATLPDGGKVRALGSIPGAPVRTGVVFRFEPTDCGMQLTFPVQAGDAIEYS
                     AFLAPRGLRTGPRRLEDANGAVTFSRPARVATEQGYASGVEPALVRARAGFDALAAGP
                     LTITHCAQPGPLPADPLAPPLPRG"
     gene            28989..29336
                     /locus_tag="VF517_14755"
     CDS             28989..29336
                     /locus_tag="VF517_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054248.1"
                     /translation="MAEEPREEESPSAGEQVKALREDLSPAKLNAKLEEVIEERPKTA
                     HLLDFRIVGQALLAAAIVALILLVLIGPKIGALALVVVFAGAWLLGAQLSYDRRRQTR
                     DARADDGAEASTG"
     gene            29357..30790
                     /locus_tag="VF517_14760"
     CDS             29357..30790
                     /locus_tag="VF517_14760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8054249.1"
                     /translation="MRTARRTWLVLGALTVVGAALRLPFLGLESLWYDETFTLSVVEQ
                     DSLGALLDRVRETESTPPLYYLVTWAFTQLVDSTGDAALRAPAALAGVLCVPASYLAV
                     RRLCGERIGLAVAALTAVSPVLVEYSLNARAYPLVVLFGCLSLWALGEAVERRGGRWL
                     AAWAVFAALALWTHYYAGFLVAAELVALLWLLPGARTRVAMAGAGLVVACLPLLSFVT
                     DQRDERASHIDSLALDERVEQALRQLAAGANPPSRVLEALAIALAIGGIAAGAWFARR
                     LRGPALLGALGAAAVAAPLLLAVTGIDDHFFMRNLLVAWACMAALAAVGLTRARGVPL
                     AAALLVAVALVIGGHADWRHQNADWQGALRSLGREAEVPIVVMPGLNHAIPNAYLRRQ
                     VAFGAVVTDQMWVLVEPGRAGRPDLRELPGFPRSTPPGFRRVETRTYRGFRLVHFVAD
                     GPTQFDPTGFGPDQIGQQTVTLTTRAP"
     gene            complement(30787..31515)
                     /locus_tag="VF517_14765"
     CDS             complement(30787..31515)
                     /locus_tag="VF517_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8054250.1"
                     /translation="MDHCPGPPPSPLRRISSERARSGTFAAVRWACLAQNDDSVTVVI
                     VEDHLALRKGLELLLSGTEFSVIGTADDAEEGQRLIEEHAPAIAIVDIGLPGRSGLEL
                     TRRLLASTPELGVLLYTGIGDQDTLVRALDSGARGFALKAGAPDELVAALRAVAAGSS
                     YVDERLRSLLLARFTTERIGVLSPREREVLDLLAQGLNGEEVAVRLVLSPETVRTHVR
                     NAMEKLEANTRVHAVAIALRQGEI"
     gene            31567..32382
                     /locus_tag="VF517_14770"
     CDS             31567..32382
                     /locus_tag="VF517_14770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEX8054251.1"
                     /translation="MSQLRPADRRRFSDAEALAIHSEYKQTGDPRLRDRLVISYAGIV
                     KHIVYRKVRELPSWCEVDDLLSCGIEALIAAIDRFDPTRGATFEQYAWTRVHGAVLDE
                     LRRQDWAPRPLRRWERDIVRARDHFFTIHARQPTSDELADALGIDPAQLDRIRRDIQT
                     SELTSLNTLVLDDDEGSVERIDTIESHEAGADPERATAVGEAKDKFRRAFGRLSERER
                     ELAVLLYVNNLTLKEIGDVLGVSESRVCRLHGRLKARLRESLRADEQLFREVA"
     gene            complement(32422..33792)
                     /locus_tag="VF517_14775"
     CDS             complement(32422..33792)
                     /locus_tag="VF517_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8054252.1"
                     /translation="MIFRQIVHDDLGCASYFVGDDDAGVAAVIDPRLDIGAYLELARY
                     LGVEIDHVLETHSHADHVSGHGRLAAATGATIHVHRDAEPGYDHAAFDDGWVLELGCV
                     HVTALHTPGHRPEHTAFTLTDTARGDEPWAVLTGDSLFVGDVARPDLAVDPAAGAHAM
                     FRTLHERLLALPDECEVWPAHLGGSLCGGPGMDMKGSSTIGYERRHNELVGIEEEDVF
                     VEHVLAGLAPQPPNFEAIVAINRGPLVTEAAEIMPLTPRQVEQKAAAGALIVDVRTDL
                     QFDEAHIPGSVSNTMLRAGFGTKLAWIADPDQEIVLVGRDDEDGRLAARLAAVVGITR
                     LGGFLAGGATSWRAEERPVARTERIGVDELHDREGEVQILDVRERDEWDAGHIPGSVH
                     APYHDIDAVPEGIDPARPVAAICMSGQRSAVAASLLERHGAERVIHVADGGIGTWRRR
                     GYPIET"
     gene            33817..34689
                     /locus_tag="VF517_14780"
     CDS             33817..34689
                     /locus_tag="VF517_14780"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX8054253.1"
                     /translation="MEIFRTPDERFEGLPGYSFEPHYEEIDGLRLHYVDEGTGDPVVC
                     LHGEPTWSYLYRKMLPPLVEAGNRVICPDLAGFGRSDKPTDRDWYTYDRHVALVTELL
                     GRLDLSGATVVVQDWGGPIGLRWAVENADRVARLVVMNTGLYTGRVSKGFMAWRDFAE
                     RTPDLPIGMIVQGATATEVPDEVVAAYEAPFPTPESKAGAARFPLIVPIDAEQPGAAE
                     MGAVMDELSRWEKPALVAFSDGDPVFPFPQSGERFTETIPSAGEQVRIEGAAHFLQED
                     RGEEIAAHIVRFLG"
     gene            complement(34771..35385)
                     /locus_tag="VF517_14785"
     CDS             complement(34771..35385)
                     /locus_tag="VF517_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054254.1"
                     /translation="MKLKPSLAVLGTAALVAFPAGAMAKSDHSGGKDKGGDKAAETSK
                     GPKKPKVSTYEFKGVVGAVGAGGVQVEVTGGNSRARGFKGQTLTFDVTKAKLKVADAN
                     GDGKRDLADVAAGDRVRVQAKLPRGAVDATGALPARQFMDRGPAAAPKAGDDDAPKAG
                     DDDAPKADDGSKADDDGSKAGDDGSKADDDAPKRGDDDDARPAA"
     gene            complement(35465..37033)
                     /locus_tag="VF517_14790"
     CDS             complement(35465..37033)
                     /locus_tag="VF517_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054255.1"
                     /translation="MSSRLRTCAALVAVIGALGAPGTASAAFDVVPSPNAYSGNNVLN
                     GVSASSPTDAWAVGSLCCSVRNSGTGALLEHWDGQAWTAVLGPDARFLDEVLNGVVAT
                     SPSDAWAVGRVKQSGYTGGTPLILHWDGSSWQPVTPPSGITGELRAVSSDTLGGAWAV
                     GDDGHGHPLALRCDVASCTQAALPQAGAVGHLRGIAAVGPNDVWAVGDSGNMTLAMHW
                     NGADWSVVASPNPDMDVNILHAVAGVSGNDVWAVGHQDQDKANTGVPPGTRTLAMHWD
                     GTGWSLASTPNVGDNDSLRGVAATGTAAITAVGTFQDASGEIPLERTLGVLWGGSDWT
                     TAATPNVGSSDNLLRAAAPIPGTPDVWAVGLHLTSGGPTQTLVLRRSPDAAPGTSDPA
                     PASDPAPASDPAAQPEAGAPLSAPAGPAVQRETTEQDTTEPEATGGPAPPHETCGSIR
                     VTVALGKAGLRASSIRVSANGVRHVLSGRHRRVKVEVPYTGTASTRVTVRIQGRGGER
                     RTITRTVTLCHPAP"
     gene            complement(37169..37252)
                     /locus_tag="VF517_14795"
     tRNA            complement(37169..37252)
                     /locus_tag="VF517_14795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37216..37218),aa:Leu,seq:cag)
     gene            complement(37296..37928)
                     /locus_tag="VF517_14800"
     CDS             complement(37296..37928)
                     /locus_tag="VF517_14800"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8054256.1"
                     /translation="MQTKTRRTDLLKASPRMFDSELLDWLSRVHPAVPVVIFLPAIVV
                     LLVLGAADLSVWATLGLFAGGYAFWTLSEYWIHRGIFHFEPEDGFGARLHWIIHGVHH
                     DHPNDPLRLVMPPSVSVPLSSLFVLAFWAVLGSPLFLPFSAGFLGGYLAYDMTHYALH
                     HHKPKSRLAKRLRELHMRHHFQDHTRGFGISAPYWDRAFGTFPKAAEKDR"
BASE COUNT         5132 a        13761 c        14091 g         5152 t
ORIGIN      
        1 ggaactggtg caggcgccgc gcgcggacga gcgtgacctc gcgcacccac tcctcggcct
       61 ccagcgcgtc gaggcgttcg agggcggcct tcgccgcggt cacccgcgcc cggatctcct
      121 cagcctcctc ctcgctcccg tcctgccgga tgccgagccg cctgatcagc accggcaggg
      181 tgagcccctg ccccacaacg gtcacgacga tcagggcgaa cgtgatgaag aggatcaggt
      241 cgcgatcggg gagggccgcg cccgcatcgg tctcgagcgg cagtgccaga gccgccgcca
      301 tcgacacggc gccgcgcagc ccggcccacg ccgtcacgat ccgcggccgg ggtcccactc
      361 gccgctgctg ctgcgccggg cgccggtcga ccgcgcggat caggtagggg accgtgaaca
      421 gccacgcgaa gcgggagccg atcaccaccg cgcacacgag cgccgcgtac ccgatcagct
      481 cgccggcccc gcggccgtcg aggtcgccga ggatcacgtg gagctgcagg ccgatcagca
      541 cgaacagcgc cgcgttcagc aggaacgtga ggatctccca cacagcgaag ccctgaagcc
      601 gcgtctccgg cgaggcgaac gtgtgggcgc gccagcccat gtagatgccg caagtgaccg
      661 cggccagcac gcccgacgcg tgcagctgct cggccgggat gaacgccgcg tacgcggtga
      721 ggagcgagat cgtgttctcg ctcgggggat cgtccaggcg ccggcggatc tcgccgatca
      781 cgaacccgac ggcgaggccg acggcgattc ccccggcggc cgcgcccacg aactcgagcc
      841 ccgcgtcggc ggcggagaag ctgccgccca ccgcagcgcc caccgccacg cggtacgcga
      901 ccagggccgt ggcgtcgttg acgaggctct cgccctccac gatgttcacc acccgccgcg
      961 gagcgccgag gcggcgcatg atcgtggtgg cggcgaccgg gtcggtgggg gagacgatcg
     1021 ctccgagggc gaacgccatc ggccaggaga gtccgatcac ctcgtgcccg acgacggcca
     1081 ccatgccgat cgtcgcgagc acgagcccga tcgcgagcat cgagatcgcc cgcacgtcct
     1141 cgcgcagcgc ccgcaggtcg gagaagaagg ccgccgagta gagcagcggc gggaggaaga
     1201 tcgtgagcac gagctcgggg tcgagctcga tctcgggcat gccgggcacg aagcccagcg
     1261 ccagcccgcc gacgaccagc ggaatggggt acgggatcgt gagccagcgc gccagcgcgt
     1321 tcaggccggc aacggccacg aacagggcga ctatgaagac ctcgaggtgc tccaccttgc
     1381 gggcgaagct atcgcccgcg ggcgactaga cctgtggcac cggctcggcc gccggcgggc
     1441 cggtgcggct cgggcgcagc agcgccagct cgtccccgag ccagtcgagc aggcaccaga
     1501 cgtccttcat ctggtcgcgg tcggccacga tcccgaagtc gaggtggccg cagtagctca
     1561 tcaccgtgat gttcaggccc atgccgtcgg tgatcaccga caccgggtag ttcgcgtcga
     1621 gccgcgcgcc cgcgcagtag agcgggaact gcgggccggg cacgttcgag accacgaggt
     1681 tccacgtcgg gcggccgatc tgggcgttgg tcgagagcct gaacgtgagc tgcgccgcgc
     1741 gcgagaagac cgccggcggg atgaagtggt tcgcgtcctg gagcagctcc gccggcagcg
     1801 cgcggtggcg ctccttcatc tccgagagcg actcgtgggt ccgcagcagg cgcttgaccg
     1861 ggtcggccac ctccgtgtgc agcggcgcgc tcatcagcat gatccggttg ccgtaggtgc
     1921 cgacctgctc gtcggtgcgc accgacaccg ggatctgcgc gacgagcggg tcgttggtca
     1981 gctcgtcgtg ctcgatcagc cagcgccgca gcgcgccggc gcagatcgag acgatcacgt
     2041 cgttcaccgt ggagccgtgc gcgttcttca cgctcttgac gtcgtcgagc gagagctggc
     2101 cgaacgcgaa gcgccggtgc ggcgagatgc gcccgttgaa cgacgtgcgc gggggcacga
     2161 gccggcggcg gtcggggatc tggccgtcgc ccgagaccag ccggcgcgcc aggccgccga
     2221 gacgcccgac ggttccggcg cccggcaggt tcgcgagcgg ggtctcctcg atgttcggga
     2281 tcgcggtcgg gagcgagcgc gccagccgca tcggatagcg cggcacgccg agcacgccgc
     2341 gggccagcag ctcgaggtcg ctcggcttgc gctcgtgctg ggccggaggc ggctcgggca
     2401 gctccctgcc ctccgggctc aggtcgagca gcaggcccat gatctccgcg ccgctcatcc
     2461 cgtcgatgac cgcgtggtgg atcttggtca gcatcgccac gtggcccgat tcgtggccgt
     2521 ggatcaggta cagctcccag agcgggcggg cgcggtcgag cgggcgcgag acgatccgcg
     2581 cgacctgctc ggcgagttgg cggtcgtcgc cggggggtgg gagcgcgagc tcccgcacgt
     2641 ggaagtcgag gtcgaagtcg gcgtcgtcga tccagtacgg gtagtcgagg cccagaggga
     2701 tctcggcgag ccgccagcgc agcggcggca ggagcgggag ccgctcggag atcaggtcct
     2761 gaatgtcgcc gcagccgagc gacccggtcg gcttggtcga cgggtcgagc atcgcgagcc
     2821 cggccacatg accggtctgg cgcgcgcttt cgagcgcgag gaactgcgag tcgagactgg
     2881 tgagctgtcg cacgtcggat cccctcccgg cgggaagcct acgcgtcacg ggcgcggtgg
     2941 cgcgccgtgg gtgaggtcgc ggctcggcgc ctagcgctcg aggatcgcca cgacgccctg
     3001 gccgccggcc gcgcagatcg agatcaggcc gcggcccgac cccttctccg cgagcagctt
     3061 ggccagcgtc gccacgatgc gcgcgcccgt ggccgcgaac gggtgacccg ccgccagcga
     3121 ggagccggcc acgttcaggc gcgagcggtc gatcgggccg agcgcggcgc cgcgccccaa
     3181 gcgctcgcgg cagaagcccg ggtcctccca cgccttcagc gtcgcgagca cctgcgaggc
     3241 gaacgcctcg tggatctcgt agtagtcgaa gtcctggagc gtcaggccgt ggcggtcgag
     3301 cagccgcggc accgcgtaga ggggcgccat cagcaggccg tcgtcgccgt gcacgtagtc
     3361 gacggcgtgc gtctcgacgt ccacgaagtg cgccagaacg ggcaggcgcc gctcggcggc
     3421 ccactcgtcg ctcgccagca ggacggcggc ggcgccgtcg gagaggggcg tcgagttgcc
     3481 agcggtcatc gtggccccgc cctcggcggt gccgaagacc gggcgcaggc tggcgagctt
     3541 ctcggcgctc gagtccgggc gcatgttctg gtcgcgctcg agcccgaggt agggcgtgac
     3601 gaggtcgtgc atgaaaccgc ggtcgtacgc ggcggcgagg ttgcggtggc tggcggcggc
     3661 cagctcgtcc tggtcgagcc ggggcacctc ccactggcgc gcggtgacag ccgcgtgctc
     3721 gcccatcgag agccccgtgc gcggctcctg gttgcgcggg atcgccggca cgatctgccc
     3781 cgggcgcagc cggccgagca gcttcacgcg ccccgcggtg ctcttctccg cgttcatgtc
     3841 catcaggacg cggcggaggt cgtcgttgat ctcgacggga gcgtcactgg tcgtgtccac
     3901 gccgcccgcg atgccggact cgatctggcc gagcgcgatc ttgttggcta cgagcgcggt
     3961 cgcctcgagg ctcgtgtcgc acgcctgctg cacgtcgtag gcgggcgtcg tgtggtcgag
     4021 cgcgctgccg agcacgcact cgcgtgtgag attgaagtcg cggctgtgct tgagcacggc
     4081 gcccgcggcc acctcgccga gccgctcgcc ctggagcgcg aagcgtgcca ccaggccgtc
     4141 gagggcggcg gtgagcatgt cctggttgga cgcgtcggcg tagggaccgt tcgagcgcgc
     4201 gaacgggatg cggttgccgc cgatcacggc cgcgcggcgc gggctgggac tgccgttgga
     4261 gctcgtcatc ggccgggcag tctagaaagc cgctagtacc ctgcccggcc gtggccgacc
     4321 gctacaccac gttcgccaac tcggggcccg ggagcttcgt caccaggcag ctcgggctgc
     4381 ctcgccccgc gcagctgcgc cgccatcagc cgggggaccc ggtggtcgac ggcgcggtgc
     4441 tgctcggcgg tgcgctcggc ggccggctcg ccgtgcccgc cgccgccgtc acgcgctcga
     4501 tcggggccac cacccacgtg cccggcgccg ctcacctgcg cgaggcggcc accgcggccg
     4561 gcccggagac cgccgccttc gacccggccg tggacgaggc gcgccgcttc gccgcaatcg
     4621 tctacgacgc atccggcgtc gaggacaccg actcgctcgc gcaggtctac gacgcgctcc
     4681 acccggtgat ccgccggctc cagccgtccg gccggctcgt ggtgctcgca accccgccgg
     4741 agaacgccga gacgccgctc cacgcggtcg cccagcgcgc gctcgagggc ttcgtccgct
     4801 cggcgggcaa ggaggtcggc gccaacgggt ccaccgccaa cctcgtgtac gtggcgcccg
     4861 gcggcgaggg cgggatggag tcgacgctgc gcttcctgct ctcgggccgc tccgcctacg
     4921 tgtccggcca gccgatccgg atcggcgcgg gcgacgcgcc ggcgccggcc gactgggagc
     4981 ggccgctcga cgggaaggtg gcgctcgtga ccggcgcctc gcgcgggatc ggggaggcga
     5041 tcgccgaggt gctcgcgcgc gacggcgcgc acgtggtcgc gctcgacgtg cccgcgcagg
     5101 gcgaggcgct cgccgcggtc gcgaaccgca tcgagggttc ctcgctccag ctcgacatca
     5161 ccgcaccgga ggccccgcgc cggctggccg accacctccg cgagcgccac ggcggggtcg
     5221 acgtcgtcgt ccacaacgcg ggtgtcacgc gcgacaagac gctcggccgg atgagcgcgg
     5281 accagtggaa cctcgtgctc gacatcaacc tctcgagcca ggtgcgcatc aacgacgtcc
     5341 tgctgaagga cgacgtcctg cgccccggcg ggcggatcgt cactgtctct tcggtgagcg
     5401 gcatcgcggg caaccgcggc caggtgaact acgcgacgag caaggccggc gtgatcgggc
     5461 tggtggacgc gctggcgccc gtggtcgccg gccgcccggc cacgatcaac gcggttgctc
     5521 ccggattcat cgagacgcag atgaccgccg cgatgccggt cgccacgcgc gagggcgggc
     5581 ggcggctcaa cagcctcgcg cagggcgggc tgccggtgga cgtggccgaa gcgatcgcct
     5641 ggctcgccgc ccccggctcc ggcggcgtga acggcaacgc cgtgcgcgtc tgcggtcagg
     5701 cgctcatcgg ggcatgaccg aggtccgcga actcgagtcg cagccgcgca tgcgggtgct
     5761 ctacccgaag gcggccgccg gtgccgcgcg gggacttctc cagcgcctgc ccggcgtggg
     5821 cggcggcgag ccggtgctgc ccgaccggga gctgcgcctc ggcgggatcg aggtcgagcg
     5881 cgaccacctc gccgagtacg accgcgtgtg cggcttccgc ctgcgcgacg tgctcccgcc
     5941 gacctatccg cacctgctcg cgttcccgct gtcgatggag ctgatgaccg ccggcgactt
     6001 cccgttcccg gtgatcggga tggtccacgt gcgcaaccgg atcgagcagc tccgcccgat
     6061 cgaggctcgc gagcgcctcg acgtgcgcgt gtgggccgat ggcctgcgcc cgcacgacaa
     6121 ggggacgcag ttcgagatgc gcgcggaggt cgccgccgcc ggggagccgg tctggcgcag
     6181 cgcgagcacc tacctgcgcc gcagcggcgg tgactccggc acgaaggacg ggggcggcga
     6241 gcgccccgac ccgccgcgcg cccaggcgaa gtggaagctc gcgggcggcg tggggcgcga
     6301 ctacgcgggg gtttcgggcg acagcaaccc gatccacctg cacccgctca cggcgcgcgc
     6361 cttcgggatg ccgcgaccga tcgcgcacgg catgtggctg aaggcccgct gcctggccgc
     6421 gctcgacggc cacctccctg acgcgctcga cgtggacgtc gccttcaagc tcccggtcta
     6481 catcccgggc acggtcagct tcgcgtcgtg gctcgacggg tcaggccgcg gattcgcgct
     6541 ccacgacgcg aagaacgaga agccgcacct gaccgggtcc gcacagccgg cttagagccg
     6601 agcagccaca acagcgtcga cgaccgctgc gcgagccggt acgcgaggag tgtggccgcg
     6661 gtcgaggcgc cgaccaggag cccgagcttc aggggaacgc ccgcaggcag tccgacgatc
     6721 ggcagcgcca agaggatgat gatcggctgg tggaggacgt agacgggcat cgccgagttg
     6781 cgcgcgaagt cgagaccgcg ctcgctcccg gcgagcagct gggcgccgta gccgagcgct
     6841 gcgacgacga tgcagagggc ggcgaccgcg gagagggagt gcagggcgat ccacgcggcg
     6901 ctgccggtcg ccggcatgcc gttgcccgcg agcagtgcca ttccgatgag cgctgagatg
     6961 ccgagcaccg ccgcgcgcct gcgttcgccg tggagcgtgc gctcgacctc ggggaagcac
     7021 gtgagcagga acccgccgac gaagaagagg ctgtagaagg cgaagttcgc ccagtcgtcg
     7081 tagaggttct ggtagccggg ccagcgcccg cgcagcccga cctggacggc ggcgagcggg
     7141 acgatcgcgg cgtagatcat ccacggcggc acgcgctcca cctgccaccc gcgcgcgtgc
     7201 acgcgccgca tggcggggag gtagagcgtc gtgaacacgt agagatagat caggaaccaa
     7261 agatgcatcc acgtgaactg gtcgaggctg gtgaagaagc tgggcaggta ctcgaggaag
     7321 gactcctcgg tgttgccctt gtggcgctgc tccacccagg cgcacaccgg gatcagcgcc
     7381 acggtgccga aggcgaacgg gaccaggatg cgctggcggc gctcccgcaa caggccgtcg
     7441 gcgccgcggc ggtcgagcga cggcacgatc gaccagccgg cgagggtgaa caggaggggc
     7501 atgtgccacg ccgagatggc gcccgtgagt gtttctacgg cctgcggccg gtcggagtcc
     7561 ttgatgtgcc aggagcccga gtcgaacggg cgggcggagt ggaagagcag cagcaggtag
     7621 acggcgccgg ctcgcaacca gtcgatatcg cgcctccgtc cggtcattgg attaaactat
     7681 caagtcgagc gtattgatga cgaccgccac ccgagagagc acccgcgacc ggctgatcac
     7741 cgctgcgatg cacctgttcg ccgaccgcgg ctatgcgcgc acgaccgtcg gcgacatcga
     7801 gtcggcggcc ggcctcgcac cgcgctcggg cgcgctctac cagtacttca agggcaagcg
     7861 cgagctgctc gacgcggccc tcgagcgcca cgtcgaggat ctcgatcaga tccagggcgc
     7921 catcgacctt ctcccgctcg gcgacctgcg cgcggaactc acgctgatgg cgcgttggaa
     7981 cctccaggac ctactgcgcc gccgcgacct acaccgcttc ctgcggaagg agggcgacca
     8041 gttcccggac ctggtcgcga aggtctacga gcggatgggg gcggggccgc tccgccgcac
     8101 cgcggagttt cttagcgggc gcctacgtga cgcgggcgcc gagccgatcg acgccgaggc
     8161 ggtggtggtc gtgatggtcc agacgatggc cgcctaccgc acccacgagg acatctacgg
     8221 ggcgccgccg ctcgacgtcg acgaggagcg cttcgtgcag gcgtgggtcg acatctccct
     8281 cgcctacgcg cggagccgcg gggtcgacgt ggcgtccagc taggcggcgg ccggctggcg
     8341 ctcgccggca tccgccgcgc gcgcggcgtt ctgctcgatc ccgtcgatca cccgcggcca
     8401 gacgccgcgc ggcaggtcgt ggcccatgcc gtcgatcagc tcgagccggg cgccggggat
     8461 cgcgcgggcg gtcgccttgc cgcccgagac gcggatcatc ttgtccttcg tcccgtggat
     8521 cacgagcgtc ggggcggtga tgcgcttcag gtcgttcgcg cggttgccgg cggcggcgat
     8581 cgccgctagc tggcgcatcg tgccggccgg gctgaggccg cgctcgtagg tgcgcgagag
     8641 cacgctgcgc agctcctcct cgtcgcgctc gaatccgggc gagccgatca cgttccagag
     8701 ctggagaccg cgctcgatgt aggcgtcctt cgacttgggg atcggcttca tgaagaacgg
     8761 gtagaccgcc gccgcgggct ggccgacgaa gcggctgccg gtggtcgaca tgatcgacgt
     8821 gaacgagcgc acgcgctcgg ggtggttgat cgcgaggatc tgcccgatca tcccgcccat
     8881 cgacgcgcca acgatgtgcg cggactcgaa cccgagctcc tcgatcagcc cggcggcgtc
     8941 gtcggccatg tccgccagcg tgtaggcgag ctggcgcggc cggcgtgtca gtagctcctg
     9001 cgtgcgcggc gggggcacgt ggtcgaagcg ggtcgagcgg cccatgtcgc ggttgtcgta
     9061 gcgggtgacg aagaagccgc gctcggcgag ctgcgtacag aagtcctcgc gccaggcgat
     9121 catctgggtg ccgagcccca tgatcagcag gaccggcgga tcaccggggt cgccgaagtg
     9181 ctcgtagcac agctcgacgc cgtttacgtt cgcgaattct tcgttcatcg cggctccagg
     9241 atcatcaaga atggttccgg ttcgtgcggt ctcgacaccc acggtaaccg ccgtggatcg
     9301 ttaccgccat cggccagggc gcatgcgctc ggccttcccc tctcatcggc cagttacccg
     9361 ccggggctcg gagccaaccg gaggaccccc cagacgggcc gtcgtcgcca cgcgcgacac
     9421 cgtagggtcc agtccgtgcg cgggattcgg atcgaggagt tcggggggcc ggaggtgctg
     9481 ctgccggcgg agctgcccga tccggaggcg ccggagggcg gtgtggtgat cgaggtgagc
     9541 gccgccggcg tgaactgggc cgatacccac caggcggaga actcgtacct ggcgccggcg
     9601 cagcttccgc tggtgcccgg cggggaggtc gtcgggaccg cgccggacgg gcggcgcgtc
     9661 gtggcgctcg tgcccgctgg cggctacgcc gaaaaggcgg tcgcgttcga ggcggcgatg
     9721 tgggaggtcc cggacggcgt gagcgacggc caggcgctcg cgctcgtgct ccagggcacc
     9781 accgcgtggc atctgctgcg cacctcggcg cagcttcgcc ccggcgagag cgtggtggtg
     9841 cacgcggcgg ccggcggcgt gggcacgatc gcggtgcagc tcgcgaagcg cttcggcgcc
     9901 gggcgcgtga tcggcctggc ctcctccgag gagaagcgcg agctggccac gcggctcggg
     9961 gccgacgcgg tggtcgactc cggcgccgag gatctcaccg ccgccctgcg cgaggcgaac
    10021 ggcggcgaac gcgtggacgt ggtgctcgag atggtcggcg gccgcgcctt cgagcagtcg
    10081 ctcgaggcgc tcgcgccgtt cgggcggctc gtgcacttcg gacaggccgg ccgcgagggc
    10141 gcgccggcgg tggaccccgg gcggctgatg ggcacgagcc gcggggtgct gggcttctgg
    10201 ctggtgcaca tgatgcgccg gcccgatctg atgagcgaag cgatggaggg gatgttcgag
    10261 atggtcatcg gtggagagct ggagccgatc gtcggcgggg agtacccgct cgcggaggcg
    10321 cggcgggcgc acgaggacat ccgctcgcgc ggcacggtcg ggaagctggt gctcacccca
    10381 tgagcggcga tctgaagctc ggctacaaca ccggctactg ggccggcggc ccgccgcccg
    10441 gcatgcagga cacgatcctc gaggccgagc ggctcggcta cgactcgatc tggaccgccg
    10501 aggcctacgg gtccgactgc ctgacgccgc tcgcgtggtg ggggtcgcgg accgagcgcc
    10561 tgcggctggg caccgcgatc gtgcagatgt cggcgcgcca gcccgccgcg acggcgatgg
    10621 ccgcgatgac gatggaccac ctctcgggcg ggcgcttcgc gctcgggctc ggcgtgtccg
    10681 ggccgcaggt ggtcgagggc tggtacggcc agtcgttcgc gaagccgctc gcgcgcaccc
    10741 gcgagtacat cggcatcctg cgcgacatct gggcgcgtca gggcccggtc accaacgacg
    10801 gcgtccacta cccgctgccg ctgcccggcg gcacgggcct cggcaagccg ctgaaggccg
    10861 gcatccatcc gctgcgcgag gacatcccgg tctacctcgg ggccgagggt ccgaagaacg
    10921 tggccctgtg tgccgagctg tgcgacggct ggctcgcgat gctgctctgc ccggacaacc
    10981 aggaccagtt cgagtcctac ctcgacgagg gcttcgcacg cgagggcgcg cggcgcacca
    11041 aggaggagtt cgacgtggcc gcgaccgtcc cgctgatcgt ctgcgacgac gtgcaggacg
    11101 gcatcgacgg catgcggccg ttctacgcgc tctacttcgg cgggatgggt gccaaggggg
    11161 ccaacttcca cgccaacgtg ccggcgcgga tgggctacga agacgaggtg cgcgagatcc
    11221 aggagctcta cctcggcggc aagaagcagg aggccgccgc ggcgatcccg gcggagctga
    11281 tcgagaagat gtcgctcgtc ggcccgctgg acaagatccg tcacgacctc gacgcctggc
    11341 gggagtcgat cgtcaccacg atgctcatcc agggcgacgc gaacactctg cgcaccgccg
    11401 ccgagctcgt gctcggctag tgcgccggat cctgctggcg ggactggtcg cggtgctggc
    11461 gctgccggcc accgcgtccg cggccgaccc ggtcacgatc tcgtgggtcg gcgacatgtc
    11521 gttctcgcgg gggcacgggc tgccgcgctc gcccgactcc gtgttcgcgc cgctgcgttc
    11581 ctcgctcgcg gcggacatcg tcacgggcaa cctcgagggc acgctcggcc gcggtggacc
    11641 gagcaagtgc ggcggcggga gcacgaactg ccatgcgttc caggcccccg cctcgtacgc
    11701 gggcgtgttc cgccgcgcgg gcttcgagat gctgaacctg gcgaacaacc actcgcgcga
    11761 cttcggcagc tccgggctcg cgcagacgcg cagcgcgctg cacggcgcgc ggatcgcgca
    11821 caccgggctg cgcggacagg tgcgggtgct cgaggccaac ggcgtgcgcg tggcgttcct
    11881 cggctacgcg ccgtatccgt ggaccggtcc gctgctcgac gtggggaagg ggcgcagcga
    11941 ggtgagggcc gcgcgggtga aggcggcccg ggtgcgcctg cgcgcccggc gcgaggtggc
    12001 cgcggcggcc tcgcgcgccg acgtggtcgt cgtgttcatg cacgcggggg cggaggggtc
    12061 cggcgcgacg cacgtgccgc acgggcgcga gacggcgttc ggcgagaatc gcggcgagac
    12121 gcgccgcttc gcgcgcacga tgatcgacgc gggcgccgac gccgtgctcg gctccggccc
    12181 gcacgtcctg cgcgggatcg agtgccgccg cggccggccg atcgcctact cgctgggcaa
    12241 cttcgtgggc taccacacgc tctcgacctc gggcgtgctg gcgctgagcg gcgtggtgcg
    12301 cctgcgcatc ggccccgacg ggaggttcct gggcggccgg ctcgtgccgg tcgagctaca
    12361 ggggccgggc gtgccgcggc tcgatcccaa gcgccgcggc gtggcgcttg tccgccggct
    12421 cagccgtcag gacttcgggt cgagcaggtg caggatcgag gccgacggca agttccgcgc
    12481 gccctagcgc ggggcgctcc ctagccggcg gcggcggtgg gcgcggcctc ggccggcgtg
    12541 gcctcggccg gcgccgcctc gacctccacc ggcacgccgt tcaggttcga catccccgcc
    12601 aggcgctcga gcgactccgg ctcagcgctc atcaggtcgt tcgcgttcgc gccgcccgcc
    12661 gcgttggcga cccgccagcc gccgcggtgg ccccagccgt ggggcagcgc gaccacgccg
    12721 ggcgtcatct cgtcggtcac gagcacgggg accgagacct ccccggacgc ggagcgcacc
    12781 cgggcgggcg cgccgtcctc caggccgcgg gccagcgcgt cgcgtgggtg catcctcagc
    12841 gcgtggcggc gctcgccgcg catcagcgcc ggcacgttgt gcatccacga gttgtgcgag
    12901 cgcagctcgc gcatgccgat cagccgcagc ggcagctcgc cctcgcccgg cgcgggctcg
    12961 gcgcccagcc gcgccacctc gccggcgatc tcgcccggcg cgaggtccac cagcgccggg
    13021 cggtggcgca cccgcccccg cagcacgccg gtcggccact cgtcgccgag caggaggccg
    13081 tgcggcgcgc gcgcgagccg gcggaggctg agcccgccgc gtcgtagtcc gaagcggtcg
    13141 ccgcgcgggc cgttgcgcag catcaggtcg agcatccggc gcggcccgat tcggacccca
    13201 gcgcgcccga gaaggcgcat cggggcgatg ctggaggcca ccacgccgag agcgcgcgcg
    13261 aaagcgtcga agatctccca ctccggccgc gcctcgccgc gcgcgggcac catcgcctcg
    13321 gtgtgctgga tgaacggccg gtagaagaac tgctggaacg cgaccggcac gtcctcgcgc
    13381 tccagccagg tcgtcgcggg caggacgtag tcggcccggc ggccggtctc ggtcatgtag
    13441 atgtcgatcg agaccagcag gtcgagcttg ccgagcgcgc gttcgagcgc ggcgccgtcc
    13501 ggcaccgact ggaccgggtt gcccgccgtc acgatgagcg cccggagctg gccgggcccc
    13561 ggtgtctcga tctccggcgc catcagcgtg gcgggcatct gcccgagcac gtccgggaag
    13621 ccgccgatac gcgagcgccg cttgccgtag gtggcgagac cagcgcgctc ggccgcgccc
    13681 tcgaggtcga gcggggaact gccgaacacg atgccgccgg ggcggtcgag gttgccggtg
    13741 accgcggcga gcgcgtcgat gaggaaggcg gtgagcgtgc cgtggcggcc gaggcacgag
    13801 ccggtccggc cgtagatcgc cgcgctcggc gctgtggcga tgtcgcgcgc gagtgcccgc
    13861 acgcgctcgg cgggcacgcc cgtccagggc tcggtcgcct ccggcgcgaa gccgttcgcc
    13921 agccgggcga ggacgccgac gccgcgcgcc tgctccctca ccgcgtcgtg gtcgaccagg
    13981 tcttcctcga agatcgtgtg cagcagcgag agcagcagcc gcgcgtcgcc gtcggggcgg
    14041 atcggcacgt ggtcgaactc gcgcgccgtc tcggtccggc gcgggtcgac caccacgacg
    14101 cgcccgccgc ggcgctcgat ctcgcggagc cggtcgcgca cgcgcgggat cgacagcacc
    14161 gagccgttcg agacgaacgg gttggccccg aacatcacga gcaggtcggt gcgcagcacg
    14221 tccgggatcg ggacggtgag cggcgagccg tacatcagcg cgctcgcggc gaagcggttg
    14281 ttcacgtcct gcgacccggc tgagtagtag tgcggcgacc cgagcgcgtc gatcagcccc
    14341 ttgccccaga ccgtgtgcga gaacgaccac gccgccgggt tgcccatgta catcccgatc
    14401 gagtcgccgc cgtgctcgtc gcgcacggcc cgcagccggt tgccgatgtc ggtcagcgcc
    14461 tcgtcccacc tgacccgctc gaactcgccg tcggcgcgcc ggcgcagcgg gtggatcacc
    14521 cggtcgggat cctcctggac ctccgggaac gcgatgccct tcgggcaggc gtagccctgg
    14581 gagagcggat gctcgcggtc ggggcgcagc ttggtcacgc gcccgtcctc caccgtggcg
    14641 accatcccgc agttcgcctc gcagatccgg cagaacgtga ttcgttcctc agcgggcatg
    14701 ggcgtatggt cgcgctttct ccgcaaggaa gtcgtcgatc agctccgcga cccgccgcgg
    14761 ctggtcctcg ggcacgtaga tgtaggagtc gtccaccagt tcgagcggcg caccgagcag
    14821 cttcgcgagg cgctcgccgt gggctagggg atagatgcgc gtgcgctcgg tggaccagac
    14881 cacgagcgcc ggcttgccga actcgggcag ccgccgcgcg gcctcctgcg tctggctcgg
    14941 gtgggtggtg gagaacgcct tgccgaagtc gcgccgcacg ccctcgtcgc gcagccccgg
    15001 ctcgacccag ccggcggcga tctcgggcgg tgggtggcgc ttgaccaccc agccgtacac
    15061 gagcggcagc cgctgcactc gccgcgcgcg cagcggcagg cccgcgatcc gcatgagccg
    15121 cgggtggcgg ccgatcggcc gcagcggctt cgaccaccag gccgggaagt tcgagaacgc
    15181 gtcgcagggg gtgagcacga gcgcgccgag ccggtccggg tggcgcgtgg cgaccatctg
    15241 cgccagcgcg ccgccggtgt cgttgccgac gagcacgacg tcgtcgagct cgagcgcgtc
    15301 caggaactcg gcgatgaggc gtgcggtgcc gtgcggggtc aggtccgcgt cggggtccat
    15361 cgcgacggtg tgcgacccga gcggccagtc gggcgcgatg cagcggtgcg attcggcgag
    15421 cggcggcacc acgttgcgcc acaggtcggc gttcgtgagg aacccgtgga cgaagacgat
    15481 cggccgcccg cggccgcgct cgcgatagcg cagctcgcca gcggagaggc gcacggagcg
    15541 ctcctcgccg agcagggggg agcggctcac cggaccgccc cttcgccgcg cgccgcggcc
    15601 gcgccctcgt cgcgcgcctc cgcgccttcg ccgcgcgcgg ctgcgcctcc ggagcccgcg
    15661 gctgcgcctc cggagcccgc ggccgcgcct ccggagcccg cggccgcccc tcccgcggcc
    15721 gcccctccca tgcccgcggc tgcgcctccg gccgcccctt cgccccgcgc gaccgccccc
    15781 gcgaacacga gcgcgaggcc ctccgcgaac gcgtcctcgc gcaccgcgtc cggctccgcc
    15841 agccgctgga acgcgatccc gtacgtgagc gcgttcgcga tcaccgccag ccggtccacc
    15901 ggcagcccgg gcgccgcctc cgcgatcatc cgctcggtcg tcgcgcggaa cgccgcgaag
    15961 cgctcgccga agcgctcgcg ccgctcgggg tcgcggaccg accaggccca gtactccacg
    16021 aacagcagga agcgctcggg ggcgcggtcg aggaacgccg tccactgccc ggcggccgcg
    16081 cgggtccgag ccgccgggtc gtcgcccgcc gcggccacgg cctccgtcac ctcgcgcgcg
    16141 tggcgcgcga cctcgtgctc gaacgccgcc aggaacaggt cctccttgcc gccgaagttc
    16201 gagtagaccg cacccgtcgt gtagcccgcg tcggccgcca cgtccccgac cgacgcgccg
    16261 tggaacccct tcgtcgcgaa cacgcgcgtg gccgccgcaa cgagctccgc gcgcgtgcgg
    16321 cgctgctgct cgacgcgcgt gcggcgctgc tccgcccgcc cctccggcgc ccgccccttc
    16381 ggctgctcgc tcccggatct cagataacga acgttatccg actacgcgag accctgcgcg
    16441 cacgccgctc gccagacgat ctccgccacc tcgtcgggat cgtcggtcac ggtgaggatg
    16501 tcgagatcct cggcgccgat cttctcgtcg cccgcgaccc gctcgcgcag ccagtcgacg
    16561 agccccgacc agtagtcgct gcccacgagg accaccggga agtcgcgtgc gcgcccggtc
    16621 tggatcagcg tgaccacctc gaacagctcg tccatcgtcc cgaacccgcc gggcagcgcc
    16681 acgaatccgc tcgcgtagcg cacgaacatc agcttgcggg tgaagaagta gtggaagtgc
    16741 agcggcaggt ccacgtacgg gttcatgccc tgctcgaacg gcagctcgat gcgcaggccg
    16801 accgaccgca cgccggcgtc gcgcgcgccg cggttcgccg cttccatcag ccccggcccg
    16861 ccgccggtga tcaccgcgat cccgcgctcg ccgatgctcc gcgcgaccgc ccggccgagc
    16921 tcgtactccg cgccgtcgcg cgatgagcgg gccgatcccc acaccgacac cgccggcccc
    16981 aggcccgaga gcgactcgaa gccggtcacc agctcgtcgt ggatgagcgc gagccgctcc
    17041 tcgtcggtct cgacggtggc gaccgcgggg ctctccgcgc tgaggatctc ctcgtccggc
    17101 gaacctggcg cacgctcctc catgcgagcc cagcctagag tgcccccgat gggtgaggac
    17161 gagaagctgg acgtcaagga ggaggtcgag ctcctccagt ccgcgctgcg gctccagtac
    17221 cgctccgcgg tgcagtacac gctggccgcg ggcagcatgg tcggcttcgc ctactcgcac
    17281 ttcgggacgt cgttgtggga gttcgcgcag gccgatctgg cggatgcgcg ccgactcgtc
    17341 gagaagatca agacactcgg cgcggacccg accaccgacg tcgcgccgct gcactacgca
    17401 gccgacccaa ccgagctgat cggccagctc atcgaggacg agaccgagtg cgccgaggcg
    17461 cttcaggcgt cgatcggccc gaccgggcgc gaggcgcgct ccgaggccat cgagcaccgc
    17521 ctcgagcaca tgatcatgcg caagcaggag cagatcgacc tgctggagcg cgcccggcgc
    17581 gagccggggt cgtagcgcgg cgccgcggcc gcggctgcgt gacgcggctc acgcttggcg
    17641 ccgccgcgtt caggcaccat accgacggta cggtcagcgg cagtaggggg agagcatgag
    17701 cacgaagcac gtggacctgt cggccgggag gatccgctac cgcgacacgg gcgaggggcc
    17761 gccgatggtg ttcgtgcacg gcctgctcgt ggacggccgg ctctggcgca aggtcacgcc
    17821 ggcgctcgag agcgagttcc gctgcatcgt ccccgacctc ccgctcggcg cccacacgat
    17881 cccgatgaag cccgacgccg acgtctcacc acccggcctc gcacgcctga tcgccgagtt
    17941 cctggagaag ctcgacctcg aggacgtgac cctcgtggcc aacgacaccg gtggcgcgct
    18001 cacgcagatc gcgatgaccc agagtcccga gcgcatcggc cgcgtcgtgc tcacgccctg
    18061 cgacgccttc gacaacttcc tcccgccgat gttccagccg ctccaggtgc tggcccgctt
    18121 ccagcccgcg ctcaccgcgt tcgtgcagcc gctgcgcctg cgaccgctcc gtcggctccc
    18181 gctcgcgttc ggctggctca ccaagcgccc gatcagcgat cccgacgtgg aggccgcctg
    18241 ggtcggcgcg ttcttcaagc gcggcgatgt gcgccgcgac gtcacccgca tcctccgcgc
    18301 catcgactcc cgccacaccc aggaggccgc cgagcgactc ggctcgttcg acaagccggt
    18361 gctgatcgcc tgggcgaccg aggacaaggt cttccccctg gagcacgccc accggctcgc
    18421 cgagcgcctg ccggacgcac gcgtcgagga gatcgccgac agttactcgt tcgtgccgga
    18481 ggaccagccg gagcggctgg cggagctgat ccgcgggttc gcgggggcgg gcgccagtaa
    18541 gggctcgggc gcggaaacgg cggcagtaaa gggaggctga cccggccgcc gcgcatcgat
    18601 tggagccttg tgtaccccat gacggagaaa ggctccaatc gatgaaaccg cggcgcccaa
    18661 ccggcctccc taagtgctcg ccgttgacgt acgcagcagg tcgagcacca acgccgcgct
    18721 ccacgcgaac gactccgacc ccagcccctc gcccgtcacg gggtggaagt actccttgaa
    18781 cccgccgtcc cggacgagcc gcagcgtggc cgcctcgatc gcctcggcgt aggccgcgaa
    18841 gcggtagcgg cgcaggccgc gggcgatcag ccagttgacg ttcacccaga ccggcccgcg
    18901 ccagtagcgc gagggcacga agccggggaa gacgcggtcg caggtcggca ccggccagcc
    18961 cgccccgccg tgcgcccaga agtgggcgct gtcgagctgg cggcacatga gctgtgcctg
    19021 ccgcgcgctc gggatcccgg cgaacagcgg cacgaagccg ccggccacgg gggcgagcac
    19081 ctgccgccgc gccacccggt cgaacgggac gtacgcgtgc agcgcgtcgt tccagaggcg
    19141 cgtgttcacc tgcgcggcgg tgcgcgcggc ctgctcgcgg aaccgcgctc cgtcctcgcc
    19201 gacggcctcg gcgatccgcg cgaggcactg ccccgccgcc accaggatcg cgttgaagca
    19261 cgggtcctcg accacgaacg cggagcgcgc gcggatctcg cgctcgtcgt agcggaagcg
    19321 cttgaaggtc tcgaccaggt gcacgtactg gtcgtacgcc gcggcggtgg gccgctcgtc
    19381 ggcgcgcgcc atcgtcaggt ccttgcgcgg gtacggcggc accgcgcccg gctctagctc
    19441 gatcgccgcg agcggcgcgt cccaggcggg cgagtcgtcc atgcccgact cccagggatg
    19501 ccggatggcc accaggccgt cgccctcggg gtcgcgctcg cggtagaggt agtcgtgcca
    19561 cgcggccagt cgcgggaaca ggcgggccag ccagtcgagg tccggctcgg gcgccagctc
    19621 gtgcacccgc caggcggcca ccgcgtgcac cggcggctgc acgatcccgg acgtcgcgcg
    19681 gccgggcgac gcgtgcggcg agacctcggt ccgccacgtc tcgggccccg ggaagtaggc
    19741 ctcgctctcg gggttgaaga cgatgtgggg gaggagcccg ttcgtccact gcgcggcgaa
    19801 cagcgtgtcg agctcgcaac gggcgcggtc gctcgcccgc tccgcgttgc caatcgccac
    19861 gaacgccgag tcccagctcc actggtgcgg atagagccgc ggactgggaa gcgtcgagga
    19921 gccgagccag ttcgcgtcga ggaccgagcc ggccgccgca gcgagctccg cgttcacatg
    19981 cggtttctag aaccggcccc ggacagagcg agcgccgccc tctcggacgg cgccgcgctc
    20041 tccccctcgc tctcgcgaca ctgggtcgcg gacaaggtca agcatctacg accgtaaccc
    20101 ggccgccgaa gtcctgtcaa gcgccgcagg ccgtcggtca gtcgcgcgac cacgggacga
    20161 gcgcccgcac ccagtcggcc gccgcgccga ccatgcgcag ctgacccgac gccgccgccc
    20221 gcaggccggc cgcgagtccg gcgcccacgc cctcgcccgg gacatcgacc tcgaagtcga
    20281 tcgcctcggg atcctcctgc gcgccgcgac gcaccgcgcc gttgcgctcg agcgcctcga
    20341 tcacctgcac gttgtcggtc gagacgatcg cgtggtagcg ctcgatcccc tcctcgcgcg
    20401 cgcgccggtt ggccgccgcc acgagctcgc tgcccatgcg ctgtccctgc cggtcgtcca
    20461 ccacggcgac ggccagctcc gccacctcgc gccgccccgg ctggcgcacc cagcgcgcca
    20521 cgccgaggat gtcgccgctc tcgggatcga ccgccaccag cgcctcgtgc ctgcgatggt
    20581 cgagctcggt gaggtagacc aggtcctcgg gcgcgagctc ctcggccggg cccatgaacc
    20641 gccgccgccg ggactcgtcg ctcagccggt ggaaggcggc gcgcaggcgc tccttgtcgt
    20701 cgggctcgat ctggcggatg gcgacgacgc tgccgtcgcg cagcgcggcg gtgatgggct
    20761 ccatgcgccg ctcgacgcta gcgacggtcg accgcgaccg cgagcagtgc gacgtcgtcg
    20821 gcgggcccct cgccgccgag catcgcctcg atcacgtggt cgcacgctgc ctcgggctcg
    20881 tggccggccg ccgcggccgc cgccgcgagc tggtcgaggc cgtccgagat cgactcccca
    20941 cgccgctcga cgagcccgtc ggtgtagagc agcacgagcg agcccggctc gagcgtgcac
    21001 gagccggtct cgcggccgcc gggcgcgggc acgccgagcg ggaccgaacg gggatggtcg
    21061 atgaagctca ccgacccgtc ggggcggacg atcagcggcg gcgggtgccc ggcgctcgcc
    21121 atctcgagcg tcagcccgtc cccgttcagc ctggccacga cggcggtggc gatgctgtcc
    21181 tcgcgcaggc gctggctctg gagcagccgg tccacgctcg ccatcgcgcg gccggcgtcc
    21241 tcgccctcca gcgccgccag gcggagcgag ttgcggagct gtcccatcag cgcggccgcc
    21301 tcgatcccgt gccccgcgac atcgccgatc gagaacgcca tgccgccgcc gctcagctcg
    21361 accacgtcga accagtcgcc gcccaccttg gcctcggcct tcgccggcag gtagcgcgtg
    21421 gcgacggtga cgccggccac ggacgggacg acgtgcggca gcaggctgcg ctggagcgtc
    21481 tcggcgatcc tgtgctcgcg ctcgtagagg cgagcgtgct cgatcgcgat cgccatccgg
    21541 tcggccacga gctgcgtgag ccggacctcc tccggcgtga acgcgcggcg cgtgtaggag
    21601 ccgagcggca tgacgcccgt gacacggccc tcgacgatca gcgggacccc catcagcgac
    21661 tccatcccgc tgccgcgcag cggcgggatc acgtccgcct cgtgcacctg ctcgagcacg
    21721 atcggccgcc gctcggccgc cacgcgcccc acgaacccct cacccggccg cagccgcagc
    21781 tgacccaggt ccgtcgtgag cccgcgcgtg gcacgcaggc gcagcgagtc gtcgtcctcg
    21841 ctaagcacga tcgccgcgaa gtccacctcc agcatctcgg tgacccggtt cagtagctct
    21901 tccagcagct cgtcgagctt caggtgcgag agcgcgacgt cggtgaccgc ctggaggcga
    21961 cgcagcgact ggctggcccg gtcggcctcg agccgcgcct cgcgctcggc ggcgtagagg
    22021 cgcgcgtggt cggtccggtg ggcctcctcg aggtcgccga tgagccgctc cacaccccgc
    22081 ctcagcgtgc cgacgaacag ccccgcgacc acgaccgtcc ccatggtgat gatccactgc
    22141 tcctcgtagg cggccggctg ctcgaccgcc agcacgaccg cgaagagcac cgtgatcagc
    22201 gcgatctgca cggcggccgc ggcccagggc aggaagtaga acgcggtgat gctcacccac
    22261 aggtagaaca tcgcgtagac gctggtccgt tggtcggtga agttgatgcc gagcgtgacc
    22321 agcacgctcg cgaacgcgag gcccgcgtgc ggcgtccagg cgggaagccg tccctgcccg
    22381 agcaggagga gcgcgccgcc gacgtacgcg agggcgaaca gcacgaacag acccggcaca
    22441 tcggtccccg gcggctgcgg gaacgccatc gagacggtcc cgagggtcgc accggcgacg
    22501 aacaggcttc cgagcgcgcg ctggaggacg gccgggtcga gcagcccgcc ctcgccgcgc
    22561 ctgatcacga gcgagagcag ccactgctcg agtgcgcgga gcctggccat cggccaccca
    22621 gcctaacccc ccggggcggc ggcgggaata gggcgcggac cccatggcgc cctcaaatcc
    22681 tggatacccg atccggttta tgtaatatgc acgcctccct actcgaccgg aaaggtgaag
    22741 atgccccgta cacgcccagc caaccacctc atcgccgccg ctctggtgct cgtgctcgcc
    22801 ctggcggcga cggcgtgcgg cggcagcggc ggcgggtcgg acgaccgcat ctcgctggtc
    22861 ggctactcga ctcccaagga ggcctacgcc aagctcaccg aggcgttcaa gcagacgccc
    22921 gagggggagg gcgtggagtt cagccagtcg ttcgcggcct cgggcgagca gtcgcgcgcc
    22981 gtggcctccg ggctgccggc ggacatcgtg gcgctctcgc tccagcccga cgtggacaag
    23041 ctcgtcgagc cgaagaaggt cgccgcggac tggaacaagg accggtacga gggcttcgtg
    23101 acccgctcgg tggtggtgct cgcggtccgc aagggcaacc cgaagaacat ccggcgctgg
    23161 gaggacctca ccagggacgg catcgaggtg atcgagccca acccgttcac gtccggcggc
    23221 gcgcgctgga acgtgatggc cgcgtacggc tcgcagaccg cgcaggggaa gtcggacgcc
    23281 gaggcccagc agttcctgaa ggacctgttc gacaacgtga ccgtgcagga caagtcggcg
    23341 cgcgactcgc tccagacgtt cggatcgggc aagggcgacg tgctcctcgc ctacgagaac
    23401 gaggcgatcg cggcgcagaa gaagggcgtc gagctcgact acgtgatccc cgaccagacg
    23461 atcctgatcg agaacccgat cgccgtgacc gaggagggca aggacaacga gaaggccacc
    23521 gccttcctga agttcctgcg ctcggaggcc ggccagagga tcttcgtaga ggagggctac
    23581 cggcccgtgc tcgagtcggt gaacgacgag aagcgcttcc cgaagcccaa gcagctcttc
    23641 gagatcggcg agttcggcgg ctggagcgag gtcaacgaca agttcttcga cccggacaag
    23701 ggcgtcatgg cggagatcaa caaggcgaag ggcgtctcga ccggtggctg aggcggccgc
    23761 catcgccgcg accggggcgc cgcgacggcg gtcgcggcgc ctgcgcgtcg cgcccggctc
    23821 cgcgctcggc atcgggctga tcacgacgta cctgtcgctg atcgtcctga tcccgctcgc
    23881 ggcggtcgtg gaccgctcgc tcgaggaggg cgtcggcgcg ttctgggagg ccgtcacggc
    23941 cccgcaggcc gtggcctcgc tcaagctcac gttcgccgtc tccctggtcg tggcggcggt
    24001 caacgccgtg accggcacga tgatcgcgtg ggtgctcgtt cgcgacgagt tccgcggcaa
    24061 gcggttcgtg aacgcgctga tcgacctccc gttcgcgatg ccgaccgtcg tggccggcct
    24121 cacgctcctg gcgctctacg ggcccaaggg tccggtcggg atcgacgtgg cctacacgcg
    24181 cgcggcggtg gcgctggcgc tgctgttcgt cacgctgccg ttcgtcgtgc gggcagtgca
    24241 gcccgtgctg atggaggtcg accgcgagat ggaggaggcg gccacctcgc tcggcgcctc
    24301 gccctggacg acgtttcgca gggtggtcct gccgagcctg cggcccgcca tcgtggccgg
    24361 ctcggggctc gcgttcgccc gcgcgctcgg cgagttcggg tcgatcgtcc tgatctccgg
    24421 caacctgccg tacaagaccg aggtcgcgtc ggttttcatc ttcggccgga tcgagacggg
    24481 cgacgtgacg accgccgcgg cggtgtcggt ggtgctgctg gccgcgtcgg tgctgctgct
    24541 cgcagcgctc tcgcggctcg gccggccgag gtgggtggac gatggccggt aggcgcgcga
    24601 tccgcggcgt cgcgctcggg tacctcgggc tgctgctggc cgcgccgatc gggatgatct
    24661 tcttccgcgc gttcgagcac gggctcgggc cgttcttcga cgcgctcacc acgccgggcg
    24721 tcacgcacgc gctctggcta accctgctgg tggtggccat cgccgtcccg gccaacaccg
    24781 cgttcgggat cggctgcgcg ctgctcctgg cgcggcgcag gttccccggg cgcacgctgc
    24841 tcgaccgcat gatcgacctg ccgttcgcga tctcgccggt ggtcgtcggt ctggcgctga
    24901 tcctcgtcta cgggcaggac ggctggctcg gcggcgcgct cgccgaccag ggcgtgcgga
    24961 tcatcttctc gacgcccggg atcgtgctcg ccacgatctt cgtctcgctg ccgttcgtgg
    25021 tgcgcgaggt gctgccggtg ctgcgcgaga tcggcgacga gcaggagcag gccgcgcaca
    25081 cgctcggcgc cagccgctgg caggcgttcc tgcgaatcac gctgcccgcg atccggcccg
    25141 ccgtgatcta cggcgtcgtg ctctcgacgg cccgcgcgct cggcgagttc ggcgcggtga
    25201 gcgtggtctc gggccggctc gagggccgca ccgagacgct caccctctac gtccagaacc
    25261 gtttcgaagg cttcgacctg accgccgcat acgccaccgc cgtgctgctc gcgctgctcg
    25321 gcatgggcgt gctcgtgaca ctcacagttc tatccaggag gcgcagtcag catgggtatt
    25381 gagaccgccg gcatccacaa gcggttcggc gagcacgtcg cgctcgagga cgtgtccgtc
    25441 ctcgtgccgg agggctccct caccgcgatc ctcggcccga gcggaggcgg caagtcgacg
    25501 ctgctgcgcg tgatcgccgg gcttgagcag cccgacgcgg gccaggtcct gatcaacggc
    25561 cgcgacgtga cgggcgatcc ggcgcaggag cgcgagatcg ggttcgtgtt ccagcactac
    25621 gcggcgttca agcacatgac cgtgcgcgac aacgtggcgt tcgggctgcg catccgcaag
    25681 cgcccgaagg ccgagatcgc ccgcaaggtc gacgagctgc tcgacctcgt ccagctcggc
    25741 acctacgtcg accgctaccc ggcccagctc tccggcggtc agcgccagcg gatggccctg
    25801 gcgcgcgcgc tggcgatcga gccgcgcgtg ctgctgctcg acgagccgtt cggagcgctc
    25861 gacgcgcgcg tgcgcaacga gctgcgtgct tggctcaagc gcctccaggc cgaggtgggc
    25921 gtgacgacgc tgttcgtcac gcacgaccag gaggaggcga tggacctcgc tgaccagatc
    25981 gtgctcgtgc gcgacggccg cgtggagcag gcgggcaccc cgcacgagct gtacgagaag
    26041 ccggcgaacg acttcgtgat gagcttcgtc gggctcgtga cgcagctcga cggcaagctc
    26101 gtacgcccgc acgacctgca gctattgccg acgccgttcg cggggacgag cgacgaggcc
    26161 gtcgtcgagc gcgtgtcgcg gctcggattc gaggtccgcg tggagctcac gctggccagc
    26221 ggcgagggcg tggtggcgca gctctcgcgc gcggaggagc gcgagctcga gctgcgcgag
    26281 ggcgacatca tccacgtgcg cggggtggac ggggcggcct cgctggccgc gtaaccggcg
    26341 cggtcccccg cgcggtcggc gcgcgggacg cggcgccccc acgccgccgg cgcgcgctcg
    26401 ccacaatgtc ggcgtgcgcg tctccgccaa gaccgactac gccctgaggg cgtcgctgga
    26461 gctcgcgcgc gccgagggcg ggcaccccgt gaaggccgac cacatcgcgc tggcccagca
    26521 gatcccggtg cgcttcctcg agaacatcct gggggagctg cgccacgcgg ggctggtcga
    26581 gagccggcgc ggggcggacg gcggctacat gctcgcgcgg ccgggcgcgg cgatcgcgct
    26641 ggccgacgtg gtgcgggccg tcgacgggcc gctcgcgaac gtgggcggcc gccggcccga
    26701 cgccctcgag tacccggccg gagcggaggc gctgcgcgac gtgtgggtgg cggtgcgcgc
    26761 cgggctgcgc gaggtgctcg agcggaccac gctcgccgat atcgccgccg ggcagcttcc
    26821 ggacgcggtg gcggcgctgg tcgagcccga ggacgcgtgg cagccgcgct gagcggctgc
    26881 gcgcgggacg gcggcggcac gcggggcggc ggcggcgcgc gggtcggcac gcggggcggc
    26941 ggcggcgcgc gggtcggcta gccgcggggc agcggcgggg ccagcgggtc ggccggcagc
    27001 ggcccgggct gggcacagtg cgtgatcgtc agcgggccgg ccgccagcgc gtcgaagccg
    27061 gctcgcgccc tcaccagcgc gggctcgacc cccgaggcat agccctgctc ggtcgccacc
    27121 cgcgccgggc ggctgaacgt gaccgcgccg ttcgcgtcct ccaggcggcg tggcccggtg
    27181 cgcagcccgc gcggcgccag gaacgcggag tactcgattg cgtcgcccgc ctggaccggg
    27241 aacgtgagct gcatgccgca gtcggtcggc tcgaagcgga acaccacgcc ggtccggacg
    27301 ggcgccccgg ggatcgaccc cagcgcgcga accttgccgc cgtccggcag cgtggcgacc
    27361 acgcgcttga cgcgcgtggg cgcgaggcgc cagccgccgc gcatcgtcac ggcgccgttg
    27421 ggcgcgaccc cgacgtcgga cgcgaacggg aacgcgggga cgccgccggt gcgcaggatc
    27481 ggccccgcgc tgtcgggatc cgcggcggtg aacgggcggt ggcggagcac gtccacccag
    27541 cggtcgctgc gcagcacctt catcgcgacg actccgaagt cgtagcgcac gtcgtacggg
    27601 tacttgccgc tgatcgtcgg gcggatcacg gcccagacgg gcccgcggcg gatcacggtg
    27661 aagcggctct cggcgtggga gagcttcgtg cgcagcgggc ggtcggcggg gatcgacacg
    27721 cggtccggcg gctcgcgcga ctcgtcgcgc gcccactcga gcagggcgag caccaggccg
    27781 ccgaacgatg ggccgccggc gccggagtcg agcccggcgg gcggcaccca gcgcgagcgc
    27841 gcgacgctcg gcgtgatctc gagcccccag tgcgtcacgc cgtgcacggt gagcaggcgc
    27901 tggagggcgc ggctcgcgag ctcgcgcagc tggccgtcct ccgccgagcc cgtctcgtcg
    27961 aggtcggccg cggcgtaggc cccgtagacg gtgccggcca gcccccaggc ctgctcctgg
    28021 ttgcgcccga agtaggcgac gtcgccgtcc ggcgcggcca gcagcagcga ggcgcgcgcg
    28081 acctcggcca gcgtgcgccg ggcggcgtcg ctggcctcgt cgcccagcag ctccactccg
    28141 cgcgcgtaga agccgagcga caggccctgg taggcgagcg ggttgtcggg cgggtcggag
    28201 agtacgaagg cgcggccgtt cggcgcggcg accgcctcgg cgcgcgccat cgccgggatc
    28261 cgcttgttga tcagctcgct gctcagccgg tcggcgttgg cgcgctggcc gcccagcacc
    28321 gaggtggggc tctccgaccg caggccggtg gcgagcagct cgcgaatctc gacggcctcg
    28381 atcagccagt ggttgccgta gtacgtcacc gcggggaggc ggatcagcgg cgcgtcgcgc
    28441 aggaagtcct cccacgccgc gcggaagcgc ttgaacagcg gctcgccggc ggcgtgctcg
    28501 cgggcgatgt tgtagccggc cgcgagcgcg aggctctcga acacgctcgg gcggttgggg
    28561 cggcggccgg gctggagcgg gttggtgagc ccgcgcaggc cggcctcgac cgcggcgtcg
    28621 tcgcccgtgc gcacgccgct catcagcagc gcgtagccca tgtaggcgtt gccgtagcgg
    28681 gtgccgcccc cgatcgagga gcggaagtgg ccgttcgggc gctggatctc ggggtagcgg
    28741 gcgcgcatcg cgtcgatcag agggtccacc ggcttctgac ccgccgccgc gcggctctcg
    28801 cccccgggcc cgtcgtcgtt cgccgagacg ccgagcgcgg cggccacggc gatcgcgagc
    28861 gccgacagcg ggattcgcag acgtctgagg cgggagcggc gcattgcgaa ggagggtatg
    28921 agagcgcggt gtttgccccg ggagcgcccg ggatacctct cgggtgtcga gcgaggagag
    28981 ggacggggat ggccgaggag ccgcgtgaag aggagagccc gagtgcgggc gagcaggtca
    29041 aggcgctgcg cgaggacctc tcgccggcga agctgaacgc gaagctcgaa gaggtcatcg
    29101 aggagcgccc gaagacggcg cacctgctcg acttcaggat cgtgggccag gcgctgctgg
    29161 ccgcggcgat cgtcgctctg atcctgctcg tgctgatcgg gccgaagatc ggggcgctcg
    29221 cgctggtggt cgtgttcgcg ggcgcctggc tgctgggcgc gcagctcagc tacgaccgcc
    29281 gccgccagac gcgcgacgcg cgcgcggacg acggcgccga ggccagcacc ggctagcccg
    29341 tcactaacct cgccgggtgc gaacggcccg gcgcacatgg ctcgtgctcg gcgcgctcac
    29401 ggtcgtcggc gcggcgctca ggctcccgtt cctcgggctc gagagcctct ggtacgacga
    29461 gaccttcacg ctcagcgtgg tcgagcagga ctcgctcggg gcgctgctgg atcgcgtccg
    29521 cgagaccgag tcgaccccgc cgctctacta cctggtgacc tgggcgttca cgcagctcgt
    29581 ggacagcacc ggcgacgccg cgctgcgggc gcccgcggct ctggccggcg tgctctgcgt
    29641 gcccgcctcg tacctcgccg tgcgccgcct ctgcggcgag cggatcggcc tggcggtggc
    29701 cgcgctcacg gcggtgagcc cggtgctcgt ggagtactcg ctgaacgcgc gcgcgtaccc
    29761 gctggtggtg ctgttcggct gcctctcgct gtgggcgctc ggcgaggcgg tggagcgccg
    29821 cggcgggcgc tggctcgccg cgtgggccgt cttcgcggcc ctggcgctct ggacgcacta
    29881 ctacgccggc ttcctcgtgg cggcggagct cgtggcgctg ctctggctac tgccgggcgc
    29941 gcgcacgcgc gtggcgatgg ccggcgcggg cctcgtggtg gcgtgcctgc cgctcctgtc
    30001 gttcgtgacc gaccagcgcg acgagcgcgc ctcgcacatc gacagcctcg ccctcgacga
    30061 gcgcgtcgag caggcgctgc gccagctcgc ggccggggcg aacccgccga gtcgtgtgct
    30121 cgaggcgctc gcgatcgcgc tcgcgatcgg ggggatcgcg gcgggggcct ggttcgcgcg
    30181 gcgcctgcgc gggcccgcgc tcctgggcgc gctcggcgcc gcggcggtgg ccgcgccgct
    30241 gctgctcgcg gtgacgggga tcgacgacca cttcttcatg cgcaacctcc tcgtcgcgtg
    30301 ggcgtgcatg gccgcgctgg cggcggtcgg gctcacgcgt gcgcgggggg tccccctggc
    30361 ggcggcgctg ctcgtggccg tggcgctcgt gatcgggggg cacgccgact ggcgccacca
    30421 gaacgccgac tggcagggcg cgctcagatc gctcggacgc gaggcggagg tgccgatcgt
    30481 ggtgatgccc ggcctcaacc acgccatccc gaatgcctac ctgcgccgtc aggtggcatt
    30541 cggggcggtt gttaccgacc agatgtgggt gctagtcgag ccgggccggg caggccgccc
    30601 ggaccttcgc gagctgccgg ggttcccgcg ctcgaccccg cccggcttcc gccgcgtcga
    30661 gacgcgcacc taccgcggct tccgcctcgt ccacttcgtg gccgacggcc cgacccagtt
    30721 cgacccgacg ggcttcggcc ccgaccagat cggtcagcag acggtgacgc tgaccacgcg
    30781 ggcgccctag atctcgccct ggcgcagggc gatcgcgacc gcgtgcacgc gcgtgttcgc
    30841 ctcgagcttc tccatcgcgt tgcgcacgtg ggtgcggacg gtctccggtg agagcacgag
    30901 ccgcaccgcc acctcctcgc cgttcaggcc ctgggccagc aggtccagca cctcgcgctc
    30961 gcggggcgag aggaccccga tccgctcggt ggtgaagcgc gcgagcagca gcgagcgcag
    31021 ccgctcgtcc acgtagctcg agcccgctgc gaccgcccgc agcgccgcca ccagctcgtc
    31081 cggtgccccc gccttgaggg cgaacccgcg cgcgccgctg tcgagcgccc gcaccagcgt
    31141 gtcctggtcg cctatccccg tgtagagcag cacgccgagc tcaggggtgg acgcgagcag
    31201 gcgccgcgtg agctcgagcc cgctgcgtcc gggcaggccg atgtcgacga tcgcgatggc
    31261 gggagcgtgc tcctcgatga ggcgctgccc ctcctccgcg tcgtccgcgg tgccgatcac
    31321 gctgaactcc gtcccgctca gtaggagctc taaacccttc ctgagtgcga gatggtcttc
    31381 cacgatcaca acggtgacgc tgtcgtcgtt ttgggccaag caggcccaac gtaccgcagc
    31441 aaacgtgccc gaccgcgccc tttccgagga aatacgccga agtggggagg ggggtggccc
    31501 cggacaatgg tccatacccc ccaagtgtcg gtcgagcgta cgtctacgga caccgacgac
    31561 ttgagtgtga gccagctacg gccagcggac aggcgccgct tcagcgacgc ggaggcgctc
    31621 gcgatccaca gcgagtacaa gcagaccggc gacccgcgcc tgcgcgatcg cctggtgatc
    31681 tcctacgccg ggatcgtcaa gcacatcgtc taccgaaagg tgcgcgagct gccctcgtgg
    31741 tgcgaggtcg acgacctgct ctcgtgcggt atcgaggccc tgatcgcggc gatcgaccga
    31801 ttcgacccga cccgcggcgc gacgttcgag cagtacgcct ggacgcgcgt gcacggcgcg
    31861 gtgcttgacg agctgcgccg ccaggactgg gcaccgcgcc cgctgcgccg ctgggagcgc
    31921 gacatcgtgc gcgcccgcga ccacttcttc acgatccacg cccgccagcc cacgagcgac
    31981 gagttggccg acgccctcgg catcgacccc gcccagctcg accggatccg gcgcgacatc
    32041 cagacctccg agctgacgtc gctgaacacg ctcgtgctcg acgacgacga gggctcggtc
    32101 gagcggatcg acaccatcga gtcgcacgag gccggcgccg acccggagcg ggccacggcg
    32161 gtgggcgagg cgaaggacaa gttccgccgc gcgttcggcc gtctgagcga gcgcgagcgc
    32221 gagctggcgg tgctgctcta cgtgaacaac ctgacgctga aggagatcgg cgacgtgctc
    32281 ggcgtctccg agagccgcgt ctgccggctg cacgggcggc tcaaggcgcg cctgcgcgag
    32341 tcgcttcggg ccgacgagca gctctttcgc gaagtggcgt agccccgcgg gtcgcgggtg
    32401 ccgtggcgcc cccggcggcg ctcaggtctc gatcgggtag cccctccgcc gccaggtgcc
    32461 gatgcccccg tcggccacgt ggatcacccg ctcggccccg tggcgctcga gcaggctcgc
    32521 cgccaccgcg ctgcgctggc ccgacatgca gatggccgcg accgggcggg cggggtcgat
    32581 cccctccggc accgcgtcga tgtcgtggta gggcgcgtgg accgatccgg ggatgtggcc
    32641 cgcgtcccac tcgtcgcgct cgcgcacgtc gaggatctgc acctcgccct cgcggtcgtg
    32701 cagctcgtcc acgccgatcc gctcggtgcg cgcgaccggc cgctcctcgg cgcgccagct
    32761 cgtcgcgccg ccggccagga agccgccgag ccgcgtgatg ccgaccacgg cggcgaggcg
    32821 cgcggcgagg cggccgtcct cgtcgtcgcg gcctaccagc acgatctcct ggtcgggatc
    32881 ggcgatccac gcgagcttcg tgccgaagcc cgcccgcagc atggtgttcg acaccgatcc
    32941 ggggatgtgc gcctcgtcga actgaaggtc ggtgcgcacg tccacgataa gcgcgccggc
    33001 ggccgccttc tgctccacct ggcggggcgt gagcggcatg atctcggcgg cctcggtcac
    33061 gagcgggccg cggttgatgg cgacgatggc ctcgaagttc ggcggctgcg gcgccagccc
    33121 ggcgagcacg tgctccacga agacgtcctc ctcctctatc ccgaccagct cgttgtggcg
    33181 gcgctcgtag ccgatcgtcg acgacccctt catgtccatc cccgggccgc cgcagagcga
    33241 gccaccgagg tgggccggcc agacctcgca ctcgtcgggg agcgcgagca gccgctcgtg
    33301 gagcgtgcgg aacatggcgt gggcacccgc ggccgggtcc accgcgaggt ccggccgggc
    33361 cacgtcgccg acgaacagcg agtcgccggt gaggacggcc cagggctcgt cgccgcgtgc
    33421 ggtgtcggtg agcgtgaacg ccgtgtgctc gggccggtgg ccgggggtgt ggagcgcggt
    33481 cacatgcacg cagcccagct cgagcaccca gccgtcgtcg aacgccgcgt ggtcatagcc
    33541 gggctcggcg tcgcggtgca cgtggatcgt cgctccggtc gcggcggcca gccggccgtg
    33601 cccggatacg tggtcggcgt ggctgtgggt ctcgagcacg tggtcgatct ccacgcccag
    33661 gtagcgcgcc agctcgaggt acgcgccgat gtcgagccgc gggtcgatca cggcggccac
    33721 gcccgcgtcg tcgtcaccca cgaagtacga cgcgcagccg aggtcgtcgt ggacgatctg
    33781 gcggaagatc acccgggcag gataagttcc cgggtgatgg agatcttccg cacgccggac
    33841 gagcgcttcg agggtctgcc tggctactcg ttcgagccgc actacgagga gatcgacggg
    33901 ctgcggctgc actacgtgga cgaggggacc ggagacccgg tcgtgtgcct ccacggcgag
    33961 ccgacctggt cgtacctgta ccggaagatg ctgccgccgc tggtcgaggc cggcaatcgc
    34021 gtgatctgcc cggacctcgc cgggttcggg cgctccgaca agccgaccga ccgcgactgg
    34081 tacacctacg accgccacgt ggcgctggtc accgagctgc tcgggcgcct cgacctgagc
    34141 ggtgcgacgg tggtcgtcca ggactggggt gggccgatcg ggctgcgttg ggcggtggag
    34201 aacgccgacc gcgtggcgcg gctcgtggtg atgaacaccg gcctgtacac gggccgggtg
    34261 agcaaggggt tcatggcgtg gcgggacttc gccgagcgca ccccggacct gccgatcggg
    34321 atgatcgtgc agggcgccac ggcgaccgag gtgccggacg aggtggtggc cgcctacgag
    34381 gcgccgttcc cgacgcccga gtcgaaggcc ggggcggctc ggttcccgct gatcgtgccg
    34441 atcgacgcgg agcagccggg ggcggcggag atgggcgcgg tgatggacga gctctcgcgc
    34501 tgggagaagc cggcgctcgt ggcgttctcg gacggcgacc cggtgttccc gttcccgcag
    34561 tcgggcgagc ggttcaccga gacgatcccc tcggccgggg agcaggtccg gatcgagggc
    34621 gcggcgcact tcctccagga ggatcgcggc gaggagatcg ccgcgcacat cgtgcgcttc
    34681 ctgggctgaa cgaccgaggg cccggcgttc ggccggaccc tcggggactt gcgagcgccg
    34741 gggggatcgg cgcccgggtt ggtggggttc ttaggcggcg ggccgagcgt cgtcgtcgtc
    34801 gccgcgcttc ggcgcatcgt cgtcggcctt cgacccgtcg tcgccggcct tcgacccgtc
    34861 gtcgtcggcc ttcgacccgt cgtcggcctt tggggcatcg tcgtcgccgg ccttcggcgc
    34921 atcgtcgtcg ccggccttcg gcgcggcggc cgggcccctg tccatgaact gccgggcggg
    34981 gagcgccccg gtcgcgtcga cggcgccgcg cggcagcttc gcctggaccc tcacgcggtc
    35041 gcccgcggcc acgtcggcga ggtcgcgctt gccgtccccg ttggcgtcgg ccaccttgag
    35101 cttggccttg gtcacgtcga atgtcagcgt ctggcccttg aagccgcggg cgcggctgtt
    35161 gccgcccgtg acctcgacct gcaccccgcc ggcgccgaca gccccgacga cgcccttgaa
    35221 ctcgtacgtc gagaccttgg gcttcttcgg gcccttgctc gtctcggcgg ccttgtcgcc
    35281 gcccttgtcc ttcccgccgg agtggtcgct cttcgccatc gcgcccgcgg ggaaggcgac
    35341 gagggcggcg gtgccgagca ccgcgagcga tggcttgagc ttcatacctc tctcaacggc
    35401 acctgcgcgc gattcctgac gcccgggcgc ggtgctggac gcatgtggga cgccgagctg
    35461 cccgttacgg agcggggtgg cacagggtca cggtccgcgt gatcgtgcgc cgctcgccgc
    35521 cgcgaccctg gatccggacg gtcacccgtg tgctggccgt gccggtgtag ggcacctcga
    35581 ccttcacgcg cctgtgccgg ccggagagca cgtgcctcac gccgttggcg ctcacccgga
    35641 tcgagctggc ccgcaggcct gccttgccga gcgcgacggt gacgcgaatc gagccgcagg
    35701 tctcatgtgg cggtgcggga ccgccggtcg cctccggctc ggtcgtgtcc tgctcggtcg
    35761 tctcccgctg taccgcgggc ccggcagggg cggacagggg cgcgccggcc tcgggttgcg
    35821 ccgcgggatc gcttgccggc gccggatcgc ttgccggcgc cggatcgctt gtcccgggcg
    35881 ccgcgtcggg cgaccggcgc agcaccagcg tctgggtcgg cccgccgctc gtgaggtgca
    35941 gtcctaccgc ccacacgtcg ggcgtgccgg gaatcggcgc cgcggcgcgg aggaggttgt
    36001 cggaggaacc gacgttcggc gtggccgcgg tcgtccagtc cgagccgccc cagagcacgc
    36061 cgagcgtgcg ctcgaggggg atctcgccgc tcgcgtcctg gaaagtcccg acggcggtga
    36121 tcgcagccgt accagtcgcc gcgacgccgc gcaggctgtc gttgtcgccc acgttcggcg
    36181 tgctcgcgag gctccagccg gtcccgtccc agtgcatcgc cagggtgcgg gtgccgggcg
    36241 gaacgcccgt gttggctttg tcctggtcct ggtggcccac cgcccacaca tcgttgccgg
    36301 acacgcccgc cacggcgtgc aggatgttca cgtccatgtc cgggttaggc gacgcgacga
    36361 cggaccagtc cgctccgttc cagtgcatgg cgagggtcat gttgccgctg tccccgacgg
    36421 cccacacgtc gttcggcccg acggctgcga tcccccgcag gtggcccacg gcgccggcct
    36481 gcggaagcgc ggcctgggtg cacgaggcga cgtcgcagcg gagagcgagc gggtggccgt
    36541 gcccatcgtc gcccaccgcc caggcgccgc ccaacgtgtc gctgctcacg gcgcgcagct
    36601 cgccggtgat tccgctcgga ggcgtgacgg gctgccagct cgacccgtcc cagtggagta
    36661 tcaggggcgt tccgccggta tagccggact gcttcacgcg cccaaccgcc cacgcgtccg
    36721 agggcgaggt ggcgacgacg ccgttcagca cctcgtccag gaagcgcgcg tcggggccca
    36781 ggaccgcggt ccaggcctga ccgtcccagt gctcgagcag ggcgccggtg cccgagttgc
    36841 gcacggagca gcagagcgac ccgacggccc acgcgtcggt gggcgagctc gcgctcaccc
    36901 cgttcagcac gttgttaccg ctgtaggcgt tggggctcgg gacgacgtcg aaggccgcgc
    36961 tcgcagtccc cggcgcgccg agcgcgccga ttaccgcgac gagggccgcg cacgtacgca
    37021 gccgtgatga catcagtccc tcctgcccgg tggaaacgtc tgccgactct gagtgatacg
    37081 cgcggtgcgg ccgcgcggtt cgatggacgc cacctgctct gtcgcgtcaa gagcgtctcc
    37141 gaccgctggc gcctgctctc ggtgacagtg cggctgagag gactcgaacc tccacggggt
    37201 tgcccccacg gcgacctgaa cgccgcgcgt ataccaattt cgccacagcc gcgtgcgcgg
    37261 cgagtgtagt ccacgccgcg cgggcgcggc ggggcctagc ggtccttctc ggcggccttc
    37321 gggaaggtgc cgaaggcgcg gtcccagtac ggcgcggaga tgccgaagcc gcgtgtgtgg
    37381 tcctggaagt ggtggcgcat gtggagctcg cgcagccgct tcgccagccg gctcttgggc
    37441 ttgtggtggt gcagcgcgta gtgggtcatg tcgtaggcca ggtagccgcc caggaagccc
    37501 gccgagaacg gcaggaacag cggcgagccc agcaccgccc agaacgccag gacgaagagg
    37561 ctcgacagcg gcacgctcac cgacggcggc atgaccaggc ggagcgggtc gttcgggtga
    37621 tcgtggtgca cgccgtggat gatccagtgc agacgcgcgc cgaacccgtc ctcgggctcg
    37681 aagtggaaga tcccgcggtg gatccagtac tcggacagcg tccagaaggc gtagcccccg
    37741 gcgaacaggc cgagggtggc ccacacgctg aggtccgcgg cgccgagcac cagcaggacc
    37801 acaatcgcgg gcaggaagat cacgacgggc accgcggggt ggacgcgcga gagccagtcg
    37861 aggagctcgg aatcgaacat ccgcggcgag gccttcagca ggtcggtgcg acgcgtcttg
    37921 gtctgcatcg gccgcgatgc tagcgccagc gacggcgggc gaaaaggacc ggggcggacc
    37981 cgcgtccggc cgcgtacgat cctccgggtg aacccgctca cgctgccact cgcgatcgtc
    38041 cgcgacccgc tgcgggccgc ggcgtcgctg gccgggttct ggctcgccgt cccgcaggcg
    38101 ctcgcggcca tcgtccgcgc gaccgagctg atggag
//
LOCUS       DASVPL010000266         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_343195, whole genome shotgun sequence.
ACCESSION   DASVPL010000266 DASVPL010000000
VERSION     DASVPL010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343195"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VF517_14805"
     CDS             complement(<1..313)
                     /locus_tag="VF517_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054257.1"
                     /translation="MTTTQIESPLARLSAEQIEELAREFDAIHDDVYEDLGDRDRRYI
                     TSMIEMHRRLAVISRVLLLASRFKPAWILGTVANSSAKILENMEIGHNVLHGQWDWMN
                     DP"
     gene            complement(330..1424)
                     /locus_tag="VF517_14810"
     CDS             complement(330..1424)
                     /locus_tag="VF517_14810"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX8054258.1"
                     /translation="MAERGATPEVPPVQRRALKAVRSLFSPLLPDDYLELINPLWSTR
                     ELRGRIERIQPETENAVTIFIKPGWEWPGHRPGQYLRIGVIVDGVHHWRAYSITSDPG
                     HPEGWISVTPKLVPEGKVTPSLVRKAKPGTIIRLGGVEGDFTLPDPPPEKLLFVSAGS
                     GITPIMSMVRHLSRCETLTDVVMLHSARTEDDVIFREQLRDLDRKHDGLCVHIQKTGE
                     DGRLSPADLDRLCPDWRAREAFVSGPSEMLDAMIEHWEGNGNPDLLHLERFQPKIGGG
                     EEGEGGKIVFSKSGEETECDGGTPILVAGEEAGLTLPFGCREGICHTCVGKLSSGKVR
                     DLRNGTVHGGDGETIRTCIHAPEGDIEIAL"
     gene            complement(1492..>2754)
                     /locus_tag="VF517_14815"
     CDS             complement(1492..>2754)
                     /locus_tag="VF517_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054259.1"
                     /translation="QDVSLWGPTHLILIGSASFAALGVWILLTEGRRARPLADGDRSG
                     ALWSRIDEFIVPGALLIGLSTFQAEYDFGLPQFRLVLAPVLMMVAAGVALVAARIRSG
                     PGGALAAVGLFLAVRGGIAALVGPALGQTTTHFPLYLAEAAAVELVALRVSTDRPLRF
                     GAIAGAAIGSAGLAAEWGWSHVWGGVPWPSSMLAETLLLAPATAVAAGLVGGFVGRAL
                     LRAPGAGPVRHGAAAAVALAVIVGVLAWGIRMPEPDPAPSAAVTLRDAGRGTVDAEIA
                     LDPPGAARDAAVLEMHAWQGGGLERVGLRPLGGGRYATTRPIPAAGDWKTVIQLEADR
                     AVLALPLYMPADAAIPAPEVPASPRFRRSFEPASEVFMRERRDDVPAGLLGTAYAVVL
                     VLWSAMIAALGWGLVRLAHQRELARSFR"
BASE COUNT          388 a          987 c         1007 g          372 t
ORIGIN      
        1 agggatcgtt catccagtcc cactgcccgt gcaggacgtt gtggccgatc tccatgttct
       61 cgaggatctt cgcgctcgag ttggccaccg tgccgagaat ccacgccggc ttgaagcggg
      121 acgcgagcag cagcacgcgc gagatcaccg ccaggcgcct gtgcatctcg atcatgctcg
      181 tgatgtagcg ccggtcgcgg tcgccgagat cctcgtagac gtcgtcgtgg atggcatcga
      241 actcgcgcgc gagctcctcg atctgctcgg cgctgagccg ggcgagcggg ctctctatct
      301 gggtggtggt catggtcctc ctggtggggt cagagcgcga tctcgatgtc gccctcgggc
      361 gcgtggatgc aggtccggat ggtctcgccg tcgccgccgt gaacggtgcc gttgcgcagg
      421 tcgcgcacct tcccggagga gagcttcccg acgcaggtgt ggcagatgcc ctcgcggcag
      481 ccgaacggca gcgtgaggcc ggcctcctcg cccgctacga ggatcggcgt gccgccatcg
      541 cactcggtct cctcgccgct cttcgagaag acgatcttgc cgccctcgcc ctcctcgccg
      601 ccgccgatct tcggctggaa gcgctcgagg tggagcaggt cggggttgcc gttgccctcc
      661 cagtgctcga tcatcgcgtc gagcatctcg gacgggcccg agacgaacgc ctcgcgcgcg
      721 cgccagtcgg ggcagagccg gtcgaggtcg gcgggcgaca ggcgcccgtc ctcgccggtc
      781 ttctggatgt ggacgcacag gccgtcgtgc ttgcgatcga ggtcgcgcag ctgctcgcgg
      841 aagatcacgt cgtcctcggt gcgcgccgag tgcagcatca cgacgtcggt cagcgtctcg
      901 cagcgcgaga ggtggcggac catgctcatg atcggcgtga tcccgctgcc ggcgctgacg
      961 aacagcagct tctcgggcgg cgggtcgggc agcgtgaagt cgccctcgac cccgccgagg
     1021 cggatgatcg tgccgggctt cgccttgcgc acgaggctcg gcgtgacctt gccctcgggg
     1081 acgagcttcg gcgtcacgct gatccagccc tccgggtgcc ccgggtcgga ggtgatcgag
     1141 tacgcacgcc agtggtgcac gccgtcgacg atcacgccga tgcgcaggta ctggccgggc
     1201 ctgtgcccgg gccattccca acccggcttg atgaagatcg tgaccgcgtt ctcggtctcc
     1261 ggctggatgc gctcgatccg cccgcgcagc tcgcgggtgg accagagcgg gttgatcagc
     1321 tccaggtagt cgtccggcag cagcgggctg aagagcgatc ggaccgcctt cagcgcgcgg
     1381 cgctggaccg gtgggacctc gggtgtagct ccgcgttcgg ccattgcgag cgcggaacgt
     1441 tacacaaaac gatgtagagg ttcggggctg agcgccaagc tggcgcgcgg cctacctaaa
     1501 tgagcgggcc agctcgcgct ggtgcgccag gcgcaccagt ccccagccga gcgccgcgat
     1561 catcgccgac cagaggacca ggaccaccgc gtaggccgtg ccgagcagcc cggccggcac
     1621 gtcgtcgcgc cgctcgcgca tgaacacctc ggaggccggc tcgaacgagc gccggaagcg
     1681 cggcgaggcg gggacctcgg gcgccgggat cgccgcgtcg gcgggcatgt agagcggcag
     1741 tgccaacacg gcgcggtcgg cctcgagctg gatcacggtc ttccagtcgc cggcggctgg
     1801 gatcggccgg gtcgtcgcgt accgcccccc gccgaggggg cgcagcccga ccctctccag
     1861 gccgccgccc tgccaggcat gcatctcgag cacggcggcg tcgcgcgcgg cgccgggcgg
     1921 gtcgagcgct atctccgcgt ccacggtgcc gcgaccggcg tcgcggagcg tgaccgccgc
     1981 cgacggcgcg ggatcgggct cgggcatgcg gatcccccag gccagcacgc cgacgatcac
     2041 cgccagggcg accgccgccg cagcgccgtg gcgcaccggg ccggcgccgg gagcgcgcag
     2101 cagcgcccgg ccgacgaacc cgccgacgag gccggcggcg accgcggttg ccggcgcgag
     2161 caggagggtc tcggccagca tcgagctggg ccacgggacg ccgccccaga catgcgacca
     2221 cccccactcg gccgcgagcc cggcgctgcc gatcgccgcc ccggcgatcg cgccgaagcg
     2281 gagcggccgg tccgtgctca cgcgcagggc gaccagctcg accgcggccg cctccgccag
     2341 gtagagcggg aagtgcgtgg tggtctggcc gagcgcgggc ccgacgagcg ccgcgatgcc
     2401 gccgcgcacc gcgaggaaca gcccgacggc ggcgagtgcg ccgcccggcc cgctgcgaat
     2461 ccgcgccgcc acgagtgcca cgccggcggc gaccatcatc agcacgggtg ccagcacgag
     2521 ccggaattgc gggagcccga agtcgtactc ggcctggaag gtcgagaggc cgatcaggag
     2581 cgcgccgggc acgatgaact cgtcgatccg cgaccagagc gcgccgctgc gatcaccgtc
     2641 ggcgagcggc cgcgcgcgcc tgccctcggt cagcaggatc cagactccca gcgcggcgaa
     2701 cgaggccgag ccgatcagga tcaggtgcgt cgggccccac aggctcacgt cctg
//
LOCUS       DASVPL010000267        19931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_343210, whole genome shotgun sequence.
ACCESSION   DASVPL010000267 DASVPL010000000
VERSION     DASVPL010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 19931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19931
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343210"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VF517_14820"
     CDS             complement(<1..496)
                     /locus_tag="VF517_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8054260.1"
                     /translation="MSEAPEIEGARGEVAAAARRLAAERLVLGTSGNISLRRGDAVAV
                     SPTGAQLAELQEAQVAVTDLDGNQIGGDLAPTSELGLHLGIHAERGDGAVIHTHSPFA
                     TALSCVLDELPAVHYDMLRFGGSVRVAPYATFGSEQLASAVHAALEGRTAALMANHGA
                     VVAAA"
     gene            615..1997
                     /locus_tag="VF517_14825"
     CDS             615..1997
                     /locus_tag="VF517_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054261.1"
                     /translation="MTLRPVLITIAVLLIAASPASATYPGHNGRLALSADLGDGRGLY
                     TMNPDGTELDLLEPAGANVFAPRWTADGAKIVYTRDAALYIANADGSGAVQIPTNGPA
                     LWGALAPGGTKVAFMRYEGGRTDVYTMDVDGTELVSHGRGNAFSGPRFSPDGKWLVFA
                     REAPGTPEPSGYVPQTYRIVTTPVEGGAEQVLTPETRHAHGPSFSPDGKTIVYSTDNE
                     VRVMGAHGGGDVALPATGGELWQSQPVFSPDGTRIAYYGSGRAGSGVYMVDPEGSSLS
                     VVPLTVRNGNLGIGWQPIPVGSPPPGPPAGQPPADLPGPPADPGPGVDPPGPRVDPPA
                     GPPCARTQQGARKRDVLTGTAAGDLLRGMGGNDLLRGGPGNDCLFGGAGHDRLSGGPG
                     NDRLNGFRGSDTLTGGPGRDRFMAGTGDDRVRAADGERDKVDCGLGRDTVVADAFDRI
                     VRCERVRIAR"
     gene            complement(2088..2161)
                     /locus_tag="VF517_14830"
     tRNA            complement(2088..2161)
                     /locus_tag="VF517_14830"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2125..2127),aa:Asp,seq:gtc)
     gene            complement(2139..3356)
                     /locus_tag="VF517_14835"
     CDS             complement(2139..3356)
                     /locus_tag="VF517_14835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX8054262.1"
                     /translation="MRRAEHLAERRRRALVLILCTVLCALITVLRFAVANPVESIGFL
                     YTIPVAMIAVEFGAGAGIGAALATLGLTVMWAETRDVPLGLVGYAARASTLAAIGGIV
                     GTLAKQRERVEQDRRRWFSMSNDLLSVANFDGRYTHVNEAWTTLLGYSRAELLGRAYT
                     DFVHPDDVERTNEEAAALGAGPHVLVNFENRYRAADGSWCWLLWSARSDSEQIYAVAK
                     DITERKRLEQEREELVARLEQAVRRDPLTGLANRRAWDDQFAVELRRAARSGEPLSLV
                     MIDLDNLKTVNDQQGHQAGDRLLRGSAAAWRECIRETDFIARIGGDEFAILLPDCAPE
                     EAQRVLGRMAEGMRDRHSFSAGVALWDGEEPSTELVRRADEALYAAKASGRGCVRVSA
                     ATLLPHSGRSSVG"
     gene            complement(3456..5144)
                     /locus_tag="VF517_14840"
     CDS             complement(3456..5144)
                     /locus_tag="VF517_14840"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8054263.1"
                     /translation="MPTPFVERLGLTEPAFPSVEGCSERGLMARALMYLFAAGGAIMA
                     GSLLLSPEGTDQLRIAITAAAAFGLALGLFLAYDRLPLWGFQVFLAAGTALIEWAIYA
                     SGETTSPFAMFYFWIAIYAFYFLSRARALIQMGFIALAYSVALFVKSDIGNVPVMHWA
                     IVTCTLVVAGALIGVQRSHVDKLIGRLSDAARTDSLTSLLNRRGFEELFETELERARR
                     GGRPLSVIVGDLDAFKAVNDRFGHAAGDRALERLSEILQSVKRRIDTAARIGGEEFAV
                     IAPDTDQHAAYILAERMRREVRDMFASEPFPLTISLGIATFPRHGATAESLIGGADEA
                     LYAAKKLGRDRTVVYNPELADTLLAATGSPTPRSERHSNTVLALAEVIDIRDSGTAAH
                     SETVGHYAGAIARELGLSADLVERVRFGGIVHDVGKIGIPDSVLRKPGWLTDDDWNEM
                     HRHPEIGARILRGANLDDISEWVLAHHERPDGRGYPHGLSGHEIPLEARILAVADAYE
                     AMTSDRVYRPALTPEVAREELQRCAGTQFDSRVVDAFVGVLGREDSAREVRVAS"
     gene            complement(5217..7331)
                     /gene="ppk1"
                     /locus_tag="VF517_14845"
     CDS             complement(5217..7331)
                     /gene="ppk1"
                     /locus_tag="VF517_14845"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX8054264.1"
                     /translation="MEPTQAAEPTEEQRDALDLEDPSLYENREISWLDFNDRVLQLAE
                     DETMPLLERAKFLAIYQSNLDEFFMVRVAGLHDQVDAGIHTPGSDGLSPSETIDRVAE
                     RVAELGARHSRQWAEGVLPELAEQSIRVVSCDACEHDELEAVDRAFDDQIFPALTPLG
                     VGPGRPFPYISNLSLSLAVWLRDPVTEAESFARVKVPKEVLPRFVPLGKRTFVPLENA
                     IARNLGKLFPGMEILSHDVFRVARDADFTVSDEADDLLEAVEQELRRRRFGEVVRLEV
                     GATMEDHLRERLVEWLKVEERQVYDVEGMLDLTDLWELYGVEGYSELRDPPHAPVTPA
                     AFQPAEEGRRADVFAAMRERDVLVHQPYESFSSSVLRFVEQAVEDPDVLAIKLTVYRT
                     DDESSLIPALIRAAEQGKQAVCMLELKARFDERRNIGWARALEEAGAHVVHGVPGLKT
                     HAKALLVVRREGDGVRNYVHVGTGNYNAKTARLYEDFGLFTTERELAADVADLFNSLT
                     GYARPQEFRKALPSPQYLRDGIIREIERTVEAHEAGSDARIVMKMNALVDARCIRALY
                     RASQAGVPVDLNVRGICRLRPGVEGVSENIRVVSVVGRFLEHSRIYGFHRGDESRWFI
                     GSADLMPRNLDSRVELVVPVESAELQVELEDTLERCFGDDTFAWVLSAAGEWQRRTRR
                     ERSVHAELVDRAQQRASGSTDA"
     gene            complement(7335..7877)
                     /locus_tag="VF517_14850"
     CDS             complement(7335..7877)
                     /locus_tag="VF517_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX8054265.1"
                     /translation="MKARRVKGLSPDMPLDEALRRIAGTRLGEVHSFDPAVRDPAAVE
                     ELHDMRIATKRLRYVLEMSEPVLGGPARLGAKRAKKLQDLLGEIHDCDEHLPLVERHI
                     ARLRAEDAAAVRAAAPRGAADLDPAAAREAPNRRRYAGLEALAAYLHARRAVLYERFV
                     RDWARLEKSGFRASLDGEVS"
     gene            complement(7874..8353)
                     /locus_tag="VF517_14855"
     CDS             complement(7874..8353)
                     /locus_tag="VF517_14855"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX8054266.1"
                     /translation="MARARPIPGFAESPTFRDAALRTVAVRAGEVFEHAEHVLDVTDI
                     ERVHDMRVATRRLRAALEIFAACFPKPEHRRVLKEVKRLADALGERRDPDVAIEAFER
                     VRDALPTAERAGVHGLIDELRARQSGANETLAAAIERVRRDGLHERLVALTAEAEPA"
     gene            complement(8356..8787)
                     /locus_tag="VF517_14860"
     CDS             complement(8356..8787)
                     /locus_tag="VF517_14860"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8054267.1"
                     /translation="MIWLLRHGEAADGSPDAERPLTERGERQSRNAGAALKALGVEPA
                     VCLTSPKVRAYDTARLACEALGIDPVHEPKLAGGPFDPEQLAAGLDDADVLLVGHDPD
                     FSMAVHSLTGAQVRMKKGGLAGLEKGELKVLLRPHELEAMA"
     gene            complement(8833..9291)
                     /locus_tag="VF517_14865"
     CDS             complement(8833..9291)
                     /locus_tag="VF517_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054268.1"
                     /translation="MRRLAPALAAVALVVLPLVGCGDDEGAALGWAVEPRVVQHPEIP
                     GDLLVTGAVRNETDDELRLDSEDARVIGSDGRALRATVRFAAGYSHSLYPPRDAPRET
                     PRQERERLGAAATIPPGGTAPLTAAWHRPRGGVRAVRIEIGDESVPLRGP"
     gene            complement(9288..10880)
                     /locus_tag="VF517_14870"
     CDS             complement(9288..10880)
                     /locus_tag="VF517_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054269.1"
                     /translation="MRIRLTVLGSLLLVLLLLPASAFAHAERATFFPDHTLASTPKYR
                     TTGPARVVCKPDSARRIKKAFGPGQKRTTRLRQLKSCKYRHIQQAVDAAVSGDRILIM
                     PGIYREEPSRAIPVKDPKCEGEGFWEASGDNHQADGRVPTFMHQAVCPNSRNLIAIIG
                     DSLDDPDRTCDHKCNLMLHGMGRKPRDVVIQNDRFKQDVIRADRADGFQVRNLTVEQA
                     GYNDINVVETNGFRLTNLVARWAKNYGILSFASDNGLYAHIDAYGNGDSGVYPGSGPE
                     GHCKRYGIEIRHVDSHDNVLGQSGTAGNGTWIHDSKFHHNAAGISADSFAPGHPGMPQ
                     DCSKWEDNEIYSNNVNFFEDQRYCNSVPFDKRPKEKVCPQFLAVVGSGFMLYGVNQNL
                     VQRNYVYDNWRSGMRLFWVPSTLRGDNNANEQTDNSNANRIVNNHFGVTPDGKAAPNG
                     KDVFWDEQGMGNCWEDNETAAGRSLTSDPLLLPTCASGGSLSPVSNPAKTAGDAPCAV
                     WSPRDNDAPPGCSWFTTPDKPE"
     gene            complement(10902..11732)
                     /locus_tag="VF517_14875"
     CDS             complement(10902..11732)
                     /locus_tag="VF517_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8054270.1"
                     /translation="MAPVRLAIVDSDSGFVTVLTKRLDAIGWHHRTLDAPVAPRELVA
                     MRLNVLVLDVTLLGQQAWDYIERVGEGVPGLGLIVCTPQSTVAQRVRGLRLGADDWIN
                     KPCHPEEVIARIEAVIRRRRRASTPAERGPVVAGELEVRADRFQVFVGGHSADLTRRE
                     FELLELLVDAHGQVLEREEIYQRVWGYAMAHGDRSVDVFVRKLRQKLEARSPGWTYIH
                     THFGIGYRFQPEPKDGGGEDLPAPEPAPSPGDEAPAAPREGLRDEPLARGRSLAGAAA
                     "
     gene            11879..12793
                     /locus_tag="VF517_14880"
     CDS             11879..12793
                     /locus_tag="VF517_14880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX8054271.1"
                     /translation="MAACASLLAACGEESGGGGGDLSGSIRTDGSSTVGPLTEAAAEG
                     FQAENSGVKVTVGTSGTGGGFEKFCAKETDVSNASRPIKDDEEVPVCKKNGVEYSELQ
                     VANDGITLVVNPKNDWAECLTVDQLAKIWSKGSKIDNWKDVDPSFPDEKLELFGAGTD
                     SGTFDFFTAEINGEEGNSRTDYNATEDDNVTVQGVAGSEGGLGYYGLEYAEQNADKNK
                     KVKVDGGAGCVEPTKETVQSGEYKPLSRPLFIYPSRQAAARPEVKAFLDFYLQNVAEL
                     ADQARFVPMTEEQLAKAQEKLGALSGGA"
     gene            12780..13694
                     /gene="pstC"
                     /locus_tag="VF517_14885"
     CDS             12780..13694
                     /gene="pstC"
                     /locus_tag="VF517_14885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX8054272.1"
                     /translation="MAALSLRAARPRYGERIVQGVLFLCALVSVAVTVGIVVALVEPT
                     IEFFREVGIGEFLGGSEWSPLFADPQFGVRDLVVGTLVTTFWALVVCIPFGLGAAIWL
                     SEYARPGARAILKPTLEVLAGVPTVVYGFFALTFVTPLLQDVWPGGDGPSIFNALAAG
                     LVMGIMILPTVASLSEDAMSAVPGGLREGALALGGSRMQVATKVVVPAALSGIVASFV
                     LAISRAVGETMIVLIAAGGVPNGGLNPLDPHQTMTGFIGAAGIGDQPTGSTGYLTIFA
                     VGTTLFVATFVMNMLSIRLVRRYREVYE"
     gene            13691..14542
                     /gene="pstA"
                     /locus_tag="VF517_14890"
     CDS             13691..14542
                     /gene="pstA"
                     /locus_tag="VF517_14890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX8054273.1"
                     /translation="MSATLRLREGLFRAALYASVAVGVIALIALVVQVLVKGWPALNW
                     GIVSNFPSSLPSKAGAQSAIVGTIWLIGLVAVTTLPLGVGAAVYLEEYANRDRWWNRA
                     IEVNIQNLAAVPSIVYGILGLAFFVRGPFGQGQVILAGGLTLSLLVLPIVIIAAREAI
                     RAVPPSIREGSLALGATQLQTIRRQVLPAAIPGIATGSILALSRAIGEAAPLVLLGAL
                     TFVTFNPDSLDSQFTALPIQIFNWISRPQEDFKVLAAAAIVVLLLILLAMNATAIWLR
                     NRYERKW"
     gene            14539..15387
                     /gene="pstB"
                     /locus_tag="VF517_14895"
     CDS             14539..15387
                     /gene="pstB"
                     /locus_tag="VF517_14895"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX8054274.1"
                     /translation="MIGIQDIRPAVETGAPATRAETNREVAIACRDVGVSYGDKPAVR
                     GITIDVYEKQITALIGPSGCGKSTLIRVFNRMNDLIPSASVQGEVIYRGEDLYGPAVD
                     PVEVRKRIGMVFQKPNPFPKSIYDNIAFGPRVLGLKDRMDERVENALRQAALWDEVKD
                     RLDSNAFGMSGGQQQRLCIARALAIEPDVILMDEPCSALDPISTGRIEDLMDELKERY
                     SILIVTHNMQQAARVSQMTAFLTVDLDAGETNRIGRLVEYDDTEKVFTNPGDPRTEAY
                     VSGRVG"
     gene            15387..16088
                     /gene="phoU"
                     /locus_tag="VF517_14900"
     CDS             15387..16088
                     /gene="phoU"
                     /locus_tag="VF517_14900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX8054275.1"
                     /translation="MSPETRIRFQEDLQALERQALGAFDMVVHAIDRTREAVQETDIE
                     LAQLVIADDDIIDGRYLEVHQSVLSLLARQAPVATDLRLIAALLDVIKHLERMGDQCV
                     NICKLLPLAGHEPPSHPTILAKIGEMASCARSQVSQAKIAFERRDVDLAEDLVRQDDA
                     IDLLNRECFKLALEIGDDPDTREWAMHMMLVARSLERIGDNAVDVGEQTAFVVTGLFR
                     EFTDASHPGGVPPIG"
     gene            complement(16085..16732)
                     /locus_tag="VF517_14905"
     CDS             complement(16085..16732)
                     /locus_tag="VF517_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054276.1"
                     /translation="MTGPAPSGHITRRSLLLAGAGAAAAAAIPDIALAAKEKVLRRSH
                     LDRSTWEPLVGTTMWVRNRGVAPVQITLVKVADLNTPKQTEAYKKKAFYLVFQGPADQ
                     PLAADTHLIKLDGIGKVPVWFSSARQIPEGWEYVAVFANSKIKARPRKKPRTKGSKEQ
                     GRRSGERAEDRRKKRRKKDTSSAPKAERRAPEPAPSDAAPAPAPAQPAESLTPAG"
     gene            16942..17370
                     /locus_tag="VF517_14910"
     CDS             16942..17370
                     /locus_tag="VF517_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8054277.1"
                     /translation="MSRDLEFELTGGPYAVTAARLALADMDDLVDEGLAFDVRLLVSE
                     LVTNSVRHAEIGPEDSIVLKLRIDDVHVRVEVSDGGPGFDPPEADPTAELARDSGWGL
                     FFVTQLADRWGIDRDDGRVWFEIERASSRAEQPARGQTAA"
     gene            17412..19133
                     /locus_tag="VF517_14915"
     CDS             17412..19133
                     /locus_tag="VF517_14915"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEX8054278.1"
                     /translation="MVLLMAFAFVAGVGTAVSPCVLPVLPLALSAGATGGPRRPLGVV
                     TGLVVTFTFAIVGLVYAIDALGLPDDLLRKVAIGGLIAFGVALTLPPVAARLEAALSR
                     LSSRVARRDARAPAAGRGGFASGLAVGAGLGLVYAPCAGPVLAGVITVSASQPFSGGR
                     LATALAYALGSAVALYALMLGGRRVTRRLSARGPRLQQALGVVMVVTAVLMLADLDLR
                     FQNAIAQDLPGFLVDPSSGLQRNDAVKRRLAELRGGPKSPLPLLAPAPEIAGTQSWFN
                     TPGERPLTLASLRAQHRVVLVDFWTYTCINCIRTLPEVTAWDRRYRRAGLTVIGVHTP
                     EFPFEKDAANVRRAIARNHIRYPVAQDNDFATWNAFSNQYWPAKYLIDAKGQVRYVHF
                     GEGKYEETEHAIRSLLAEATGRPLGPMTRARAEQPDPGLSTPETYLGSKRAEGFVDGP
                     LEPGVRDFGEGRPRLDLNQLALRGRWTIAPEASRAESRARLHLRFRARRVFLVLGSPN
                     GPRDVRVLLDGRPLPARQAGADVRGGRARVERHRLYRLVDLPRAGDHELTLQLDRGIE
                     GYAFTFG"
     gene            19157..19573
                     /locus_tag="VF517_14920"
     CDS             19157..19573
                     /locus_tag="VF517_14920"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8054279.1"
                     /translation="MEVQLMTVTGEGYAVGSLDDMGEGYGFRKVRHELGVKELGVNAI
                     VMPSGFETGNHYHEEQEELYFVHRGSIEMIFGDGSSHRLDAGGFARVDAPTVRRVKNV
                     GEEDAVYVVFGAKGGYVGRDGKLPEGEEHRVRPPGD"
     gene            complement(19729..>19931)
                     /locus_tag="VF517_14925"
     CDS             complement(19729..>19931)
                     /locus_tag="VF517_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054280.1"
                     /translation="RERQPLARALVVDLDLGYAEFLALGYDLRATQRELVAAGLPKGA
                     AHLAPSRARRVRRRLRALARGA"
BASE COUNT         2770 a         7088 c         7170 g         2903 t
ORIGIN      
        1 cggccgcggc cacaacggcg ccgtggttgg ccatcagcgc ggcggtgcgc ccctccagcg
       61 cggcgtggac ggcggacgcg agctgctcgg agccgaacgt ggcgtagggc gcgacccgca
      121 ccgagccgcc gaagcgcagc atgtcgtagt ggacggcggg cagctcgtcg aggacgcagg
      181 agagggccgt ggcgaacggc gagtgcgtgt ggatcaccgc gccgtcgccg cgctcggcgt
      241 ggatgccgag gtggagcccg agctccgagg tgggcgcgag gtcgccgccg atctggttgc
      301 cgtcgagatc ggtcaccgcc acctgcgcct cctgcagctc ggcgagctga gcccccgtag
      361 ggctcacggc gaccgcgtcg ccgcggcgca ggctgatgtt gccggaggtg ccgagcacga
      421 gccgctcggc cgccagccgc cgcgcggcgg ccgccacctc gccgcgcgcg ccctcgatct
      481 cgggtgcctc gctcatgcgc ggaacactaa gcgacggggg acccacgccg gccgcaggtg
      541 cggtgcgatc ggcgcgcctc gcctacccct ttgtacaaat tgcgcatagc ggtcggcgca
      601 tgtaccttcg tgacatgaca cttcgccctg tcctgattac gatcgctgtg ctgctgatcg
      661 ccgcctcgcc cgcgtcggcg acctatccgg gtcacaacgg gcgccttgca ctgagcgccg
      721 acctcggcga cggcaggggg ctctacacca tgaacccgga cgggacggag ctcgatctgc
      781 tcgagcccgc gggggcgaac gtcttcgccc cccggtggac cgccgacggc gcgaagatcg
      841 tctacacccg ggacgcggcg ctctacatcg cgaacgccga cggcagcggc gcagtccaga
      901 taccgaccaa cgggccggcc ctgtggggtg cgctcgctcc cggcggcacc aaggttgcgt
      961 tcatgcgata cgagggcggg cggacggacg tctacaccat ggatgtcgac ggcacggagc
     1021 tcgtgagcca cgggaggggc aacgcgttca gcggaccgcg cttctcgccc gacggaaagt
     1081 ggctggtctt cgcgcgtgaa gccccaggca ctcccgagcc cagcggctac gtcccgcaga
     1141 cctaccgcat cgtgacgaca ccggtcgaag gtggcgcgga gcaggtgctc acgcccgaga
     1201 cacggcatgc ccacggcccg tcgttctccc cggacggcaa gaccatcgtc tacagcaccg
     1261 acaacgaggt gagggtcatg ggcgcccacg gcggcggtga cgtcgcgctg ccggccacgg
     1321 gaggggagct gtggcagagc cagccggtgt tctcgccgga cggaacccgg atcgcctatt
     1381 acggcagcgg ccgcgcgggc agcggcgtct acatggtcga ccccgaaggg tcgagcctga
     1441 gcgtcgttcc gctcacggtc aggaacggaa acctcggcat cggctggcaa cccatcccgg
     1501 tcggcagccc cccgccaggc ccgccggcgg gccagcctcc ggccgatctt ccgggtccgc
     1561 ccgcggatcc cggtccgggg gtcgatccgc ccggtccgcg ggtcgacccg cccgccgggc
     1621 cgccgtgcgc gaggacccaa cagggcgccc ggaagcgcga cgtgctcacc ggcaccgccg
     1681 caggagacct cctccgcgga atgggcggca acgacctgct gaggggcgga cccggcaacg
     1741 actgtctgtt cggcggcgct ggccacgacc gcctctccgg cggccccggg aacgaccgtc
     1801 tcaacggttt ccgcggcagc gacacgctga ccggcgggcc cggccgcgac cgcttcatgg
     1861 ccggcacggg cgacgaccgt gttcgcgccg ccgacggcga gcgggacaag gtcgactgcg
     1921 ggctcggccg cgacaccgtg gtggcggacg cgttcgaccg gatcgtccgg tgcgagcgcg
     1981 tgcgcatcgc gcgctgatcc ggcccctcac ctcccgggac ttccccctct tcccccacag
     2041 cggctggtcg tgccgcactc cgaagccgga tctggctatg gaatcaacgc gagcgacggg
     2101 actcgaaccc gcgacctccg gcgtgacagg ccggcgctct aaccaactga gctacgcccg
     2161 ctgtggggca ggagggtagc ggccgatacg cggacgcagc cgcgcccgct cgccttggct
     2221 gcgtacagcg cctcgtcggc acgccgcacc agctcggtgc tgggctcctc gccgtcccag
     2281 agggcgacgc ccgcggagaa cgagtggcga tcgcgcatcc cctcggccat ccggccgagc
     2341 acgcgctggg cctcctcggg ggcgcagtcg ggcagcagga tcgcgaactc gtcgccgccg
     2401 atccgcgcga tgaagtcggt ctcgcggatg cactcgcgcc acgcggccgc tgacccccgc
     2461 agcaggcggt cgccggcctg gtgcccctgc tggtcgttga ccgtcttcag gttgtcgaga
     2521 tcgatcatca ccagggagag cggctccccg gagcgcgcgg cccggcgcag ctcgacggcg
     2581 aactggtcgt cccaggcgcg gcggttggcc agtccggtga gcgggtccct gcggacggcc
     2641 tgctccagcc gcgccacgag ctcctcgcgc tcctgctcga gtcgcttgcg ctcggtgatg
     2701 tccttcgcca ccgcgtatat ctgctcgctg tcggatcggg cgctccagag cagccagcac
     2761 cagctcccgt cggccgcgcg gtagcggttc tcgaagttca ccagcacatg cggacccgcg
     2821 ccgagggcgg cggcctcctc gttcgtgcgc tccacgtcgt cgggatgcac gaagtcggtg
     2881 taggcccgcc ccagcagctc tgcccgcgag tagccgagga gcgtggtcca cgcctcgttg
     2941 acgtgcgtgt agcggccgtc gaagttcgcc acgctcagga gatcgttcga catcgagaac
     3001 cagcgtctgc ggtcctgctc gacgcgctcg cgctgcttgg cgagggtgcc gacgatgccg
     3061 ccgatcgcgg ccagcgtcga ggcccgtgct gcgtagccca ccaggccgag cgggacatcg
     3121 cgggtctctg cccacatgac cgtcagccca agggtcgcca gcgcggcccc gatcccggcg
     3181 ccggcaccga actcgaccgc gatcatcgcg acggggatcg tgtagagaaa cccgatcgac
     3241 tccaccgggt tcgccaccgc gaagcgaagc acggtgatca gggcgcacag gaccgtgcac
     3301 aggatcagga cgagcgcgcg gcggcggcgc tccgcgaggt gctcggctcg tcgcatcccg
     3361 cggtctatcg gcgttcgctg gacacatatt gagcgtagaa acgcgaagcc ccccgagcgg
     3421 gggtcatccg attcggggag ggcgaggcgg gctggctagc tcgccacgcg cacttcgcgc
     3481 gccgagtcct cgcgaccgag cacgccgacg aaggcgtcca ccacgcggga gtcgaactgc
     3541 gttccggcgc agcgctggag ctcctcgcgc gcgacctccg gcgtgagcgc gggccggtag
     3601 acgcggtcgc tcgtcatcgc ctcgtaggcg tcggccaccg cgaggatgcg cgcctcgagc
     3661 gggatctcgt ggccgctcag cccgtgcggg tagccgcgac cgtcggggcg ctcgtgatgg
     3721 gcgagcaccc actcgctgat gtcgtcgagg ttcgcgccgc gcaggatccg cgcgccgatc
     3781 tcggggtggc ggtgcatctc gttccagtcg tcgtcggtga gccagcccgg cttgcgcagc
     3841 acggagtccg ggatgccgat cttgccgacg tcgtgcacga tgccgccgaa gcggacccgc
     3901 tcgaccaggt cagcggacag cccgagctcg cgggcgatcg cgccggcgta gtggccaacc
     3961 gtctccgagt gcgcggcggt gccggagtcg cggatgtcga tcacctcggc gagcgcgagc
     4021 accgtgttgc tgtggcgctc ggagcgcggc gtcggcgacc cggtcgcggc gagcagcgtg
     4081 tcggccagct cggggttgta gaccaccgtg cggtcgcggc ccagcttctt ggccgcgtag
     4141 agcgcctcgt cggcgccgcc gatcagcgac tcggcggtcg cgccgtggcg cgggaaggtc
     4201 gcgatcccga gcgagatcgt gagcgggaac ggctcggacg cgaacatgtc gcggacctcg
     4261 cggcgcatgc gctcggcgag gatgtacgcg gcgtgctggt cggtgtcggg cgcgatcacg
     4321 gcgaactcct cgccgccgat ccgcgccgcg gtgtcgatcc gccgcttgac cgactggagg
     4381 atctcgctca ggcgctcgag cgcacggtcg ccggccgcgt ggccgaagcg gtcgttcacc
     4441 gccttgaagg cgtcgaggtc accgacgatc acgctgagcg gccggccgcc gcggcgcgcc
     4501 cgctcgagct cggtctcgaa cagctcctcg aagccgcggc ggttgagcag gctcgtgagg
     4561 ctgtcggtgc gcgcggcgtc ggagagccgc ccgatcagct tgtcgacgtg gctgcgctgc
     4621 acgccgatca gcgccccggc gaccacgagc gtgcaggtga cgatcgccca gtgcatgacc
     4681 ggcacgttgc cgatgtcgct cttgacgaac agcgccacgc tgtaggccag cgcgatgaag
     4741 cccatctgga tcagggcccg ggcgcggctc aggaagtaga acgcgtagat cgcgatccag
     4801 aagtagaaca tcgcgaacgg actcgtcgtc tcgccgctgg cgtagatcgc ccactcgatc
     4861 agggcggtcc ccgccgcgag gaatacctgg aagccccaga gcggcaggcg gtcgtaggcg
     4921 aggaacagcc cgagcgcgag gccgaaggcg gccgcggccg tgatcgcgat ccgcagctgg
     4981 tcggtgccct cgggcgagag caggagcgag cccgccatta tcgcgccgcc cgcggcgaag
     5041 agatacatga gcgcccgcgc catcaacccg cgctcggagc agccctcgac ggacgggaag
     5101 gcaggctcgg tgaggccgag gcgttctacg aagggggttg gcatgcgttt gcccactatc
     5161 ggtcccccgt ccgcacggcg ccaccaacat ccgtccagtc gaggttttcg cggcgcctag
     5221 gcgtcagtgc tgccggaggc gcgctgctgg gcgcgatcca ccagctcggc gtgcacgctg
     5281 cgctcgcggc gcgtgcgccg ctgccactcg ccagcggcgc tgagcaccca cgcgaacgtg
     5341 tcgtcgccga agcagcgctc gagcgtgtcc tcgagctcga cctggagctc cgccgactcg
     5401 accggcacca ccagctccac gcggctgtcg aggttgcgcg gcatcaggtc ggcggatccg
     5461 atgaaccagc ggctctcgtc gccgcggtgg aagccgtaga tccgcgagtg ctcgaggaag
     5521 cgcccgacca ccgacacgac ccggatgttc tcgctgacgc cctcgacgcc cggtcgcagg
     5581 cggcagatcc cgcgcacgtt gaggtcgacc ggcaccccgg cctgcgacgc gcggtagagc
     5641 gcgcggatgc agcgcgcgtc cacgagcgcg ttcatcttca tgacgatccg cgcgtcggac
     5701 ccggcctcgt gcgcctcgac cgtcctctcg atctcgcgga tgatcccgtc gcgcaggtac
     5761 tgcggcgacg ggagcgcctt gcggaactcc tgcggccggg cgtagcccgt cagcgagttg
     5821 aacaggtctg cgacgtcggc ggccagctcg cgctcggtcg tgaacagccc gaagtcctcg
     5881 tacagccgcg ccgtcttggc gttgtagttg ccggtcccga cgtgcacgta gttgcgcacc
     5941 ccgtcgccct cgcggcggac cactagcagg gccttcgcgt gggtcttgag ccccggcacg
     6001 ccgtgcacca cgtgcgcccc ggcctcctcg agcgcgcgcg cccagccgat gttgcggcgc
     6061 tcgtcgaagc gggccttcag ctcgagcatg cagacggcct gcttgccctg ctcggcggcc
     6121 cggatgagcg ccgggatcag cgacgactcg tcgtcggtgc ggtacacggt gagcttgatc
     6181 gcgagcacgt ccgggtcctc gaccgcctgc tccacgaacc gcagaacgct cgacgagaac
     6241 gactcgtacg gctggtggac cagcacgtcg cgctcgcgca tcgccgcgaa cacgtccgcg
     6301 cgccggccct cctccgcggg ctggaacgcc gccggcgtga ccggcgcgtg cggcgggtcg
     6361 cgcagctcgg agtagccctc gacgccgtag agctcccaca ggtcggtcag gtcgagcatt
     6421 ccctcgacgt cgtagacctg gcgctcctcc accttcagcc actcgaccag ccgctcgcgc
     6481 aggtggtcct ccatcgtggc gccaacctcg agccgcacga cctccccgaa gcggcgccgg
     6541 cgcagctcct gctcgacggc ctcgagcagg tcgtcggcct cgtcggagac cgtgaagtcg
     6601 gcgtcgcggg ccacgcggaa cacgtcgtgg ctcaggatct ccatccccgg gaagagcttg
     6661 ccgaggttgc gcgcgatcgc gttctcgagc gggacgaagg tcctcttccc cagcgggacg
     6721 aagcgcggca gcacctcctt cggcaccttc acgcgcgcga acgactcggc ctcggtgacc
     6781 ggatcgcgca gccagaccgc caggctcagc gagaggttcg agatgtacgg gaacggccgc
     6841 ccgggcccga cgcccagcgg ggtgagggcc gggaagatct ggtcgtcgaa ggcgcggtcg
     6901 acggcctcga gctcgtcgtg ctcgcacgca tcgcaggaga cgaccctgat gctctgctcg
     6961 gccagctcgg ggagcacgcc ctcggcccac tggcgcgagt ggcgcgcgcc gagctcggcg
     7021 acgcgctcgg ccacgcggtc gatggtctcc gacggcgaga ggccgtcgct gccgggggtg
     7081 tggatgccgg cgtcgacctg gtcgtgcagc cccgcgaccc ggaccatgaa gaactcgtcg
     7141 aggttggact gatagatcgc gaggaacttg gcgcgctcca gcagcggcat ggtctcgtcc
     7201 tcggcgagct ggagcacgcg gtcgttgaag tcgagccacg agatctcgcg gttctcgtag
     7261 agcgacggat cctcgaggtc gagtgcgtcg cgctgctcct cggtcggctc ggcggcctgg
     7321 gtcggctcca tggttcagct cacctccccg tcgagggacg cgcggaaacc ggacttctcg
     7381 agccgcgccc agtcgcgcac gaagcgctcg tacagcaccg cccggcgcgc gtgcaggtag
     7441 gcggcgaggg cctcgagccc ggcgtagcgg cgccggttgg gcgcctcgcg cgcggcggcg
     7501 gggtcgaggt cggccgcgcc ccgcggcgct gcggcgcgca cggcggcggc gtcctcggcg
     7561 cgcagccgcg cgatgtgtcg ctcgaccagc gggaggtgct cgtcgcagtc gtggatctcg
     7621 ccgagcaggt cttggagctt cttcgcgcgc ttcgcgccga gcctggcggg cccgccgagc
     7681 accggctcgc tcatctcgag cacgtagcgc aggcgcttgg tggcgatgcg catgtcgtgc
     7741 agctcctcga cggcggcggg gtcgcggacc gcggggtcga acgagtggac ctcgccgagg
     7801 cgggtgccgg cgatccgccg aagcgcctcg tcgagcggca tgtccgggct gaggcccttg
     7861 accctgcgcg ccttcacgcg ggctcggcct ccgcggtgag ggccacgagc cgctcgtgca
     7921 ggccgtcgcg ccggacgcgc tcgatggcgg cggcgagggt ctcgttggcg ccggactggc
     7981 gggcgcgcag ctcgtcgatc agcccgtgca cgcccgcgcg ctcggcggtc ggcagcgcat
     8041 cgcgcacgcg ctcgaacgcc tcgatagcca cgtcgggatc gcggcgctcg cccagcgcgt
     8101 cggccagccg cttgacctcc ttcagcaccc tccggtgctc cggcttcggg aagcaggccg
     8161 cgaagatctc gagcgccgcg cgcagccgcc gcgtggccac ccgcatgtcg tgcacgcgct
     8221 cgatgtcggt cacgtccagc acgtgctcgg cgtgctcgaa gacctcgccg gcgcgaaccg
     8281 cgacggtgcg cagcgcggcg tcgcgaaagg tgggcgactc ggcgaagccg gggatcgggc
     8341 gcgcccgcgc cacggctacg ccatcgcctc gagctcgtgc gggcgcagca gcaccttcag
     8401 ctcgcccttc tcgagccccg cgaggccgcc cttcttcatc cgcacctgcg ctccggtgag
     8461 cgagtgcacc gccatcgaga agtcggggtc gtggcccacg aggagcacgt cggcgtcgtc
     8521 gaggccggcg gccagctgct cgggatcgaa cggcccgccg gcgagcttcg gctcgtgcac
     8581 cgggtcgatg ccgagggcct cgcacgcgag ccgggccgtg tcgtaggcgc gcaccttcgg
     8641 gctcgtgagg cacacggcgg gctccacgcc gagggccttc agcgccgcgc ccgcgttgcg
     8701 cgactggcgc tcgcccctct cggtcagcgg acgctccgcg tcggggctcc cgtccgcggc
     8761 ctcgccgtgg cggagcagcc agatcatggt ttggactgta cccgccgggg ggccgccttc
     8821 cggcagcgca aactagggcc cgcgcagcgg caccgactcg tcgccgatct cgatccgcac
     8881 cgcgcgcacc ccgccgcgcg ggcggtgcca ggcggcggtc agcggggcgg tgccgcccgg
     8941 cgggatcgtc gcggcggcgc cgagccgctc gcgctcctgg cgcggcgtct cacgcggcgc
     9001 gtcgcgcggc gggtagagcg agtgcgagta gccggccgcg aaccggaccg tcgcgcgcag
     9061 cgcccgtccg tccgacccga tcacgcgcgc gtcctcgctg tcgagccgca gctcgtcgtc
     9121 ggtctcgttg cgaacggcgc cggtcacgag caggtcgccg gggatctccg gatgctgcac
     9181 cacgcggggc tcgaccgccc agccgagtgc cgccccctcg tcatcgccgc agccgacaag
     9241 aggcagcacc acgagcgcga ccgccgcgag ggcgggcgcg agccgcctca ttccggctta
     9301 tcgggagtgg tgaaccagga gcagccgggg ggcgcgtcgt tgtcgcgcgg gctccagacg
     9361 gcgcacggcg cgtcgcccgc ggtcttcgcg ggattcgaca cgggcgagag cgacccgccg
     9421 gacgcgcacg tcggaagcag cagcggatcg gaggtcagcg agcggccggc ggcggtctcg
     9481 ttgtcctccc agcagttgcc catcccctgc tcgtcccaga acacgtcctt gccgttcggc
     9541 gccgccttgc cgtcgggggt cacgccgaag tggttgttca cgatccggtt ggcgttcgag
     9601 ttgtcggtct gctcgttggc gttgttgtcg ccgcgcaggg tcgacggcac ccagaacagc
     9661 cgcatgcccg atcgccagtt gtcgtagacg tagttgcgct ggacgaggtt ctggttcacg
     9721 ccgtagagca tgaagcccga cccgacgacg gcgaggaact gcgggcagac cttctccttc
     9781 gggcgcttgt cgaacggcac cgagttgcag tagcgctggt cctcgaagaa gttcacgttg
     9841 ttcgagtaga tctcgttgtc ctcccacttc gagcagtcct gcggcatgcc gggatgcccc
     9901 ggcgcgaacg agtccgcgga gatgccggcc gcgttgtggt ggaacttcga gtcgtggatc
     9961 caggtgccgt tgcccgccgt gccggactga ccgagcacgt tgtcgtgcga gtcgacgtgc
    10021 cggatctcga tgccgtagcg cttgcagtgc ccctcggggc cagaccccgg gtacacgccc
    10081 gagtcgccgt tgccgtaggc gtcgatgtgc gcgtacaggc cgttgtcgct ggcgaacgag
    10141 aggatcccgt agttcttggc ccagcgcgcg acgaggttgg tgagccggaa gccgttcgtc
    10201 tcgacgacgt tgatgtcgtt gtagcccgcc tgctcgacgg tcaggttgcg gacctggaag
    10261 ccgtcggcgc ggtcggcgcg gatcacgtcc tgcttgaacc ggtcgttctg gatgacgacg
    10321 tcgcgcggct tgcgccccat gccgtgcagc atcaggttgc acttgtggtc gcaggtgcgg
    10381 tcggggtcgt cgagcgagtc gccgatgatc gcgatcaggt tgcgggagtt cgggcacacg
    10441 gcctggtgca tgaacgtggg cacccgcccg tccgcctggt ggttgtcgcc ggacgcctcc
    10501 cagaacccct cgccctcgca cttcgggtcc ttcaccggga tcgcgcggct cggctcctcg
    10561 cggtagatgc cgggcatgat caggatccgg tcgccggaca ccgcggcatc gaccgcctgc
    10621 tggatgtggc ggtacttgca gctcttcaac tggcgcagcc gcgtggtgcg cttctggccg
    10681 ggcccgaagg ccttcttgat ccgccgcgcc gagtccggct tgcagaccac gcgcgcggga
    10741 ccggtggtcc ggtacttcgg cgtcgaggcg agcgtgtggt ccgggaagaa cgtggccctc
    10801 tcggcgtggg cgaacgcgct cgcggggagg agcaggagga cgaggaggag cgacccgagg
    10861 acggtcagcc ttatgcgcac gcgcgcaacc tacccgaagc ctcaggcggc ggcgccagcc
    10921 aggctgcgac cacgcgccag aggctcgtcg cgcagcccct cgcgcggggc cgcgggcgcc
    10981 tcgtccccgg gggagggcgc cggctccggt gccggaaggt cctcgccgcc gccgtccttc
    11041 ggctcgggct ggaagcggta cccgatcccg aagtgcgtgt ggatgtaggt ccacccgggc
    11101 gagcgcgcct cgagcttctg cctgagcttg cgcacgaaca cgtcgaccga gcggtcgccg
    11161 tgcgccatcg cgtaccccca cacgcgctgg tagatctcct cgcgctcgag cacctgccca
    11221 tgcgcgtcga cgaggagctc gagcagctcg aactcgcgcc gcgtgaggtc cgcgctgtgg
    11281 ccgcccacga agacctggaa ccggtcggcg cggacctcga gctcgccggc cacgaccggg
    11341 ccgcgctcgg ctggcgtgct cgcgcgcctg cgccgccgga tgaccgcctc gatgcgggcg
    11401 atcacctcct ccgggtggca cggcttgttg atccagtcgt ccgcgccgag gcgcagcccg
    11461 cgcacgcgct gcgcgaccgt cgactgcggg gtgcacacga tcagcccgag gccgggcacg
    11521 ccctcaccca cgcgctcgat gtagtcccac gcctgctggc cgagcagcgt cacgtcgagc
    11581 acgagcacgt tcagccgcat cgcgacgagc tcgcgcggcg ccaccggcgc gtcgagcgtc
    11641 cggtggtgcc agccgatcgc gtcgagccgc ttggtcagca ccgtcacgaa tccactgtcg
    11701 gaatcgacga tcgcgagccg gacgggggcc atcccggatg agtctatgcc gcaggacccc
    11761 tcgatccggc ggcgttcacg ggttcttcac aactggataa ccgccgggta accggatgcg
    11821 ggccgcgggc ggcgacgctg cggtcatgaa aacaagacag atgaggacat tggcggcaat
    11881 ggccgcatgc gcgtcgctcc tcgcggcgtg cggagaggaa agcggaggcg gagggggcga
    11941 cctctccgga tccatccgca ccgacggctc gagcacggtc ggaccgctga cggaggctgc
    12001 cgccgagggc ttccaggccg agaactccgg agtcaaggtc acggtcggca cgtccggcac
    12061 cgggggcggc ttcgagaagt tctgcgcgaa ggagaccgac gtctcgaacg cgtcgcgtcc
    12121 gatcaaggac gacgaggagg tcccggtctg caagaagaac ggggtcgagt actccgagct
    12181 ccaggtggcg aacgacggca tcacgctcgt agtgaacccg aagaacgact gggccgagtg
    12241 cctgaccgtc gaccagctcg cgaagatctg gagcaagggc tcgaagatcg acaactggaa
    12301 ggacgtcgac ccgagcttcc ccgacgagaa gctggagctc ttcggcgccg gcaccgactc
    12361 gggcacgttc gacttcttca ccgccgagat caacggcgag gagggcaaca gccggaccga
    12421 ctacaacgcg accgaggacg acaacgtgac cgtccagggc gtggccggct cagagggcgg
    12481 gctcggctac tacggcctcg agtacgccga gcagaacgcg gacaagaaca agaaggtcaa
    12541 ggtcgacggc ggcgcgggct gcgtcgagcc gacgaaggag accgtgcaga gcggcgagta
    12601 caagccgctg tcgcggccgc tcttcatcta cccgagcagg caggccgccg cgcggcccga
    12661 ggtcaaggcg ttcctggact tctacctcca gaacgtcgcc gagctggccg accaggcccg
    12721 gttcgtcccg atgaccgagg agcagctggc gaaggcgcag gagaagctcg gggcgctgag
    12781 tggcggcgct tagcctccgg gcggcccgcc cccgctacgg cgagcggatc gtgcaggggg
    12841 tcctgttcct ctgcgcgctc gtctcggtgg cggtgacggt cgggatcgtg gtagccctgg
    12901 tcgagccgac gatcgagttc ttccgcgagg tgggcatcgg cgagttcctc ggcggctcgg
    12961 agtggtcgcc gctgttcgcg gatccgcagt tcggcgtccg cgacctcgtg gtggggacgc
    13021 tcgtcacgac gttctgggcg ctcgtcgtct gcatcccgtt cggactcggc gccgcgatct
    13081 ggctcagcga gtacgcgagg ccgggcgccc gggcgatcct caagccgacg ctcgaggtgc
    13141 tggccggcgt gcccaccgtc gtctacgggt tcttcgcgct cacgttcgtc acgccgctgc
    13201 tccaggacgt ctggccgggc ggcgacgggc cgtcgatctt caacgcgctg gccgcgggcc
    13261 tcgtgatggg gatcatgatc ttgccgaccg tcgcctcgct gtccgaggac gccatgtcgg
    13321 ccgtccccgg gggtctgcgc gagggcgcgc tggcgctcgg cggctcgcgc atgcaggtcg
    13381 cgaccaaggt ggtcgtgccc gccgcgctgt cgggcatcgt cgcctcgttc gtgctcgcga
    13441 tctcgcgcgc ggtcggggag acgatgatcg tcctgatcgc cgccggcggc gtgccgaacg
    13501 gcggactcaa cccgctcgac ccgcaccaga cgatgaccgg cttcatcggc gcggccggga
    13561 tcggcgacca gccgaccggg agcaccgggt acctgacgat cttcgcggtc ggcacgacgc
    13621 tgttcgtggc gaccttcgtc atgaacatgc tcagcatccg gctggtccgc cgctaccgcg
    13681 aggtgtacga gtgagcgcca cgctccgcct gcgcgagggc ctgttccggg ccgccctcta
    13741 cgccagcgtc gcggtcggcg tgatcgccct gatcgcgctg gtcgtccagg tgctcgtgaa
    13801 gggctggccc gcgctgaact gggggatcgt ctcgaacttc ccgtcgagcc tgccgtcgaa
    13861 ggccggtgcg cagtcggcga tcgtgggcac gatctggctg atcggcctcg tggcggtcac
    13921 gacgctgccg ctgggcgtcg gcgccgcggt ctatctcgag gagtacgcca accgggaccg
    13981 ctggtggaac cgcgcgatcg aggtcaacat ccagaacctc gccgcggtgc cgtcgatcgt
    14041 ctacggcatc ctcggcctcg cgttcttcgt gcgcggcccg ttcggtcagg gccaggtgat
    14101 cctcgccggc ggcctgacgc tgtcgctgct ggtgctgccg atcgtgatca tcgccgcgcg
    14161 cgaggcgatc cgcgcggtgc cgccgtcgat ccgggagggc tcgctggcgc tcggagccac
    14221 ccagctccag acgatccgcc gccaggtgct ccccgccgcg atccccggca tcgccacggg
    14281 ctcgatcctc gcgctctcgc gcgcgatcgg cgaggccgca ccgctcgtgc tgctcggcgc
    14341 gctgaccttc gtgaccttca acccggactc gctcgacagc cagttcacgg cgctcccgat
    14401 ccagatcttc aactggatct cgcgccccca ggaggacttc aaggtgctcg ccgccgcggc
    14461 gatcgtcgtg ctcctcctga tcctgctcgc catgaacgcc accgcgatct ggctgcgcaa
    14521 ccgctacgag aggaagtggt gatcggtatc caggacatca ggcccgccgt cgagaccggt
    14581 gcgccggcga cgcgcgcgga gaccaaccgc gaggtcgcga tcgcgtgccg cgacgtgggc
    14641 gtctcctacg gcgacaagcc cgcggtgcgc ggcatcacga tcgacgtcta cgagaagcag
    14701 atcacggcgc tgatcggccc gtcgggctgc ggcaagagca cgctgatccg cgtcttcaac
    14761 cgcatgaacg acctgatccc gtcggccagc gtgcagggcg aggtgatcta ccgcggcgag
    14821 gacctctacg gcccggccgt ggacccggtc gaggtgcgca agcggatcgg gatggtgttc
    14881 cagaagccca acccgttccc caagtcgatc tacgacaaca tcgcgttcgg cccgcgcgtg
    14941 ctcggcctca aggaccgcat ggacgagcgc gtggagaacg cgctacgcca ggccgcgctg
    15001 tgggacgagg tgaaggaccg cctcgattcg aacgcgttcg gcatgtccgg cgggcagcag
    15061 cagcggctct gcatcgcacg cgcgctcgcg atcgagcccg acgtgatcct catggacgag
    15121 ccgtgctccg cgctcgaccc gatctcgacc gggcggatcg aggacctgat ggacgagctg
    15181 aaggagcgct actcgatcct gatcgtgacg cacaacatgc agcaggccgc gcgcgtgtcg
    15241 cagatgacgg cgttcctgac cgtcgacctc gacgccggcg agaccaaccg gatcgggcgg
    15301 ctggtggagt acgacgacac cgagaaggtg ttcaccaacc cgggcgaccc gcgcacggag
    15361 gcgtacgtca gcggacgggt cggctgatgt cgcccgagac gcggatccgc ttccaggagg
    15421 acctccaggc gctcgagcgg caggcgctcg gggcgttcga catggtggtc cacgcgatcg
    15481 atcgcacgcg cgaggccgtg caggagaccg acatcgagct cgcgcagctc gtgatcgccg
    15541 acgacgacat catcgacggc cgctacctcg aggtgcacca gagcgtgctg tcgctcctgg
    15601 cccgccaggc accggtggcg accgacctgc ggctgatcgc cgccctgctc gacgtgatca
    15661 agcacctcga gcggatgggc gaccagtgcg tgaacatctg caagctgctg ccgctcgccg
    15721 gccacgagcc gccctcccac cccacgatcc tcgcgaagat cggggagatg gcgagctgcg
    15781 cgcgctcgca ggtgtcacag gccaagatcg ccttcgagcg ccgcgacgtg gacctggccg
    15841 aggacctcgt ccgccaggac gacgcgatcg acctgctcaa ccgcgagtgc ttcaagctcg
    15901 cgctcgagat cggggacgac cccgacacgc gcgagtgggc gatgcacatg atgctcgtgg
    15961 cgcgctcgct cgagcggatc ggcgacaacg cggtggacgt cggcgagcag accgcgttcg
    16021 tggtcacggg gctgttccgc gagttcacgg acgcctcgca ccccggcggg gtgccgccga
    16081 tcggctagcc ggcgggggtg aggctctcgg cgggctgcgc cggtgcgggt gccggtgcgg
    16141 cgtcgctcgg tgccggctcg ggcgcgcggc gctcggcctt cggcgcgctg ctggtgtcct
    16201 tcttcctgcg cttcttcctg cggtcctcgg cgcgctcgcc gctgcgccgg ccctgctcct
    16261 tcgagccctt cgtgcgcggc ttcttacgcg ggcgggcctt gatcttcgag ttggcgaaga
    16321 ccgcgacgta ctcccaaccc tcggggatct ggcgcgccga cgagaaccac acgggcacct
    16381 tgccgatgcc gtcgagcttg atcaggtgcg tgtccgcggc gagcggctga tcggccgggc
    16441 cctggaagac gaggtagaac gccttcttct tgtacgcctc ggtctgcttt ggcgtgttga
    16501 ggtcggcgac cttcacaagc gtgatctgga ccggagccac gccgcggttg cggacccaca
    16561 tcgtggtgcc caccagcggc tcccaggtgg agcggtccag atggctgcgc cgcagcacct
    16621 tctccttggc cgccagggcg atgtccggga tcgcggcggc ggccgcggcg ccggcaccgg
    16681 cgagcagcag ggaacgtcgt gtgatgtgcc cagagggcgc gggtccagtc attccagtca
    16741 gaacgagcgc cgggggccga gaagacgctc tccatcgacc ctacgtcagg tttctatcgg
    16801 cgtcaatgag ccggaccaca acccggcacg agggcccggc ggaagcgcgc cctgcaagag
    16861 ctcgcccggg gtcggggatt cctctggggc cgctctgggg aataatcctg gcgtgcccat
    16921 ccacggctgg tgccggcgca gttgagtcgg gacctcgagt tcgagctgac cggtggtccc
    16981 tacgcggtca cggccgcgcg cctcgcgctg gccgacatgg acgacctcgt cgacgaggga
    17041 ctcgccttcg acgtgcggct gctcgtgagc gagctcgtga ccaacagcgt gcgccacgcg
    17101 gagatcggcc ccgaggactc gatcgtcctg aagctcagga tcgacgacgt gcacgtgcgg
    17161 gtcgaggtct ccgacggcgg gcccgggttc gacccgccgg aggccgaccc gaccgccgag
    17221 ctcgcccgcg acagcggctg ggggctgttc ttcgtcaccc agctcgccga ccgctggggc
    17281 atcgaccgcg acgacgggcg cgtctggttc gagatcgagc gcgcgagcag ccgcgcggag
    17341 cagcccgcgc gcggccagac ggcggcctga cgccggcgta cccgccggcc ctttcgtaga
    17401 tactcgacgg cgtggtcctc ctgatggcgt tcgcgttcgt cgcgggcgtc ggcaccgccg
    17461 tctcgccgtg cgtcctgccc gtgcttccgc tcgcgctgtc ggccggggcg accggggggc
    17521 cgcgccggcc gctgggcgtc gtgaccggcc tggtggtcac gttcacgttc gcgatcgtcg
    17581 ggctcgtcta cgcgatcgac gcgctcgggc tgccggacga cctgctgcgc aaggtcgcga
    17641 tcggcggatt gatcgcgttc ggggtcgcct tgacgctgcc gccggtcgcg gcccggctcg
    17701 aggcggcgct gtcgaggctc agctcgcggg tggcgcggcg cgacgcgcgt gcgcccgccg
    17761 ccgggcgcgg tggcttcgca tcggggctcg ccgtcggtgc cgggctcggg ctcgtctacg
    17821 cgccgtgcgc gggaccggtc ctcgcgggcg tgatcaccgt gtccgcgtcg cagccgttca
    17881 gcggcggccg cctcgcgacg gccctcgcgt acgcgctcgg ttcggccgtc gccctgtatg
    17941 ccctcatgct cggcggccgt cgcgtgaccc ggcgcctgtc cgcgcgcggg ccccggctcc
    18001 agcaggcgct gggggtcgtg atggtcgtca ccgccgtgct gatgctcgcc gacctcgacc
    18061 tgcgcttcca gaacgcgatc gcacaggacc tgcccggctt cctcgtcgac ccgtcgagcg
    18121 ggctccagcg caacgacgcc gtcaagcgca ggctcgccga gctgcgcggc ggtccgaaga
    18181 gcccgctgcc gctgctggcg ccggcccccg agatcgcggg cacgcagagc tggttcaaca
    18241 cgcccggcga gcgcccgctc acgctcgcga gcctgcgcgc gcagcaccgc gtagtcctcg
    18301 tcgacttctg gacctacacg tgcatcaact gcatccgcac cctgcccgag gtgaccgcct
    18361 gggacaggcg ttaccgccgc gccggcctca ccgtcatcgg ggtccacacg cccgagttcc
    18421 cgttcgagaa ggacgccgcc aacgtgcgtc gcgcgatcgc ccgcaaccac atccgctacc
    18481 cggtggccca ggacaacgac ttcgccacct ggaacgcgtt ctcgaaccag tactggccgg
    18541 ccaagtacct gatcgacgcg aagggccagg tgcgctacgt gcacttcggc gaggggaagt
    18601 acgaggagac cgagcacgcg attcgctcgc tgctcgcgga ggccacaggg cgcccgctgg
    18661 gccccatgac ccgtgcgcga gccgagcagc cggaccccgg cctctccacc cccgagacct
    18721 acctgggctc caagcgcgcg gagggattcg tggacggccc gctcgagccg ggcgtgcgcg
    18781 acttcggcga gggacggccg cggctcgacc tgaaccagct cgcgctgcgc gggcgctgga
    18841 cgatcgcgcc ggaggcctcg cgcgcggagt cgcgcgcgcg gctgcacctg cgctttcgcg
    18901 cgcgccgggt cttcctcgtg ctgggctcgc cgaacgggcc gcgcgacgtg cgcgtactgc
    18961 tcgacggccg gccgctgcct gcgaggcagg cgggcgccga cgtgcgcggc gggcgcgccc
    19021 gggtggagcg ccaccgcctc taccggctcg ttgacctccc gcgtgcgggc gaccacgagc
    19081 tgacgctcca gctcgaccgc ggcatcgagg gctacgcctt cacgttcggg taggttgcgt
    19141 actacgagta cgcaacttgg aggtccagct tatgacggtg acgggcgagg gttacgcggt
    19201 cggcagcctc gacgacatgg gcgagggata cgggttccgg aaggtcagac acgagctggg
    19261 cgtgaaggag ctcggagtca acgcgatcgt catgccgtcc ggcttcgaga cgggcaacca
    19321 ctaccacgag gagcaggagg agctctactt cgtgcaccgc ggctcgatcg agatgatctt
    19381 cggcgacggc tcctcgcacc ggctcgacgc gggcggcttc gcgcgcgtgg acgcgccgac
    19441 ggtccggcgg gtcaagaacg tgggcgagga ggacgcagtc tacgtcgtct tcggcgcgaa
    19501 gggcgggtac gtcgggcggg acggcaagct gccggagggc gaggagcacc gggtccgccc
    19561 acccggagac tgacgcgcgg cgagcgctcg gctcgctacc agtcgccttc ggttgtctcg
    19621 gggtttccgc ccatagcttc cattcgagtc ctaccacctg aactcggcag gtaaccccga
    19681 gtttcggggt tgggcggttt ttccctggcc gcggcgccga acggcgcgtc aggcgccccg
    19741 cgcgagcgcc cggaggcgcc tgcggacgcg gcgcgcacgg ctcggcgcga ggtgcgccgc
    19801 gccctttggc aggccggccg ccaccagctc gcgctgcgtc gcccgcaggt cgtagccgag
    19861 cgccaggaac tccgcgtagc cgaggtcgag gtcgaccacc agcgcgcgag cgagcggctg
    19921 gcgctcgcgc g
//
LOCUS       DASVPL010000268         8940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_110593, whole genome shotgun sequence.
ACCESSION   DASVPL010000268 DASVPL010000000
VERSION     DASVPL010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110593"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1644)
                     /locus_tag="VF517_14930"
     CDS             complement(37..1644)
                     /locus_tag="VF517_14930"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8054281.1"
                     /translation="MGATAAHCPVQFTTAHEGTELVQRTRRRLNRLGILANGLGAFDT
                     FFFLTFLLPPTFDGYDSTSAIAVNAVVFAVLLPTTFVLGTRWGYRASDSLRDWVGVRD
                     PTPAEREQALSIAYVQAAMAARFWILGAVIFSTLNVVGGIAAGAGALGGAIVGLTLIL
                     GGITTSGVHYLAAERMLRPVTTLALSAAPPVAPSGPGVGVRVLMVWLLATGTPLLGLS
                     AVGVSGLFADIDGELLAGGVLFLAMVAGFSGLIATKLTATSLSESLAGMRSALGRIEE
                     GDYDVQVPVDDSSEIGLVQVGLNRMAAGLAERERLQDLFGRHVGRDVARAALDGGVKL
                     GGEVREVGVLLVDLVGSTWMATRLPPEQVVALLNRFFSLVVEVVESYGGMVNKFEGDG
                     ALCVFGAPVAREDPAGAALCAARELRARLTDELPDVDAGIGVSAGEAVAGNVGAEERF
                     EYTVIGDPVNEAARLSELAKRRPERLVASGSAVDCASGEERGRWLAGEEEILRGRDEP
                     TRLAVVATRSGSRGSAAPRDAADAPRR"
     gene            complement(1748..3613)
                     /locus_tag="VF517_14935"
     CDS             complement(1748..3613)
                     /locus_tag="VF517_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8054282.1"
                     /translation="MSRLLHPLAAALVVLAVAAPAAGAAPETWPPPQGPGHLFVHYGE
                     EHWNDDDGMTLLPKVVADTIRYRPKLVTMSGDKANDGTVDQLAMWRQIMSAYDKAGIP
                     YFAGVGNHDRLNAPANQPGFPPGGSTTNYREVFAERPWPMGDAKPYADPSFTVHERPE
                     SDPPGAATHYSVDYGNVRWIFVDNSCWDITFCSNNQQNPADGDARTQLDWLESRALEG
                     TRSGQVVFVVMHMGTRDPRDQSYVDPTSTNHTMGKGATASDIADFERIVSTTEVDGVF
                     VGHIKGQFQYVASDVPYYIDGGAGGELYTEGPVGTDHGYWHGYRLVRVNGRNVITDSV
                     PIFVPGSLKLNGPDTLAPGAGGQFEAFGMQPVYNDPAKVPALELRDPDPIPREGVRAG
                     GLPGALIWGAPLVAFLLFGAVVTRPRGRVRVARALAPGLAGAIAVTGIAFAQRSEPTS
                     TPRDALPNPARIWTSGNPHVLAPAPSDTDDPRRDPATQTQDGRFKAGCPGRTTVRITS
                     GWESKGKRVVVPSAPGPMVKSYGKRARSLRAGTSPTLAKLVLAQPAEVEVRVTRGRRR
                     VAEPLHRCLRSGRGYAVRWNGRIGKRKAARGVYRVETWVRSDRGPSVYVTNVRVR"
     gene            3661..6054
                     /locus_tag="VF517_14940"
     CDS             3661..6054
                     /locus_tag="VF517_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX8054283.1"
                     /translation="MGKRLGSSALVCAVIAMTLAADAAATPVCTDGYKGGPPVSQCGG
                     RIFPEAHLTQGYVQYTPNPQGFAEYRHGLEYLAQKYPRWISVFTLKDRYGKDAVSAGP
                     DGIRPYEPGDTGDGRDILVVKITDHETPDAGKQPLTFSLSVHGNERGGLEGGVRTAED
                     LALAATNGGAVPDGVANYDTTTGRKPAFHTYEVRELLKKEAVYLIDFNLDGWAIGDFF
                     NIPTQPYARGNSKGTDINRQMPTLGSINTTRNPLQESEARSGLQFMNDVAASGPGGKM
                     AYGADVHGELTSQAYVDVMYPAGQFDSVDHRRLMSIAERTKSVIDATLYQGIIDQTEN
                     AGGGNEGEPGGDIPTKPAHWGTVWDTLGYTDTGFIGDYMATDLGVTGMDYEIFLNHTV
                     PDKVWNVYLQENHINATRGIIKTAMAYARTQEQEFNAQNVKVDPKGRVGYVVNPDTVT
                     DKDENGPGTLPGPKKDGKGADGQPVQQRSYDVTNQQWFADTNKLMPQSFARFASGDVA
                     RDAKALNAVDTLVLADVPLPQDTRARKVDDAAYYRNVKSWVESGGNLVLTDRAVHALH
                     AMRVVPQEAVQDVRVYQPYANISDFQHSLVEGLRPNARQLVEAAILGYGIGNNASPMT
                     IVDRAAWEAAGGKTVATTGNNSGDSDDGTRASIGELKLGKGLIRIIGGALPMPTEEND
                     HRYGLRDYGPTYSGLFVLENSLRHDVPELGRTAGAAACIKGSSRTVPISRKVRGERVR
                     STRVNANGPTRLTRRNGRLYAIVDIRKLKGKTLVLRISRRTASGKLVKTKHTRLVCR"
     gene            complement(6092..6385)
                     /locus_tag="VF517_14945"
     CDS             complement(6092..6385)
                     /locus_tag="VF517_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054284.1"
                     /translation="MLLTLLAALAVTGCAEDDGPTPLSEAEACRAVKEKLTLEQLEDR
                     FGKPDASQDFFGDRVVAYQDDEVKWQFQVGTRNGTFRALQVKGQREEVVDCPV"
     gene            6422..7009
                     /locus_tag="VF517_14950"
     CDS             6422..7009
                     /locus_tag="VF517_14950"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8054285.1"
                     /translation="MGEAPIFYYDTNSPYAYLAAERIQDVITEAEWRPIAFGMLLRDI
                     DRVPWSLRPGREEQMADCERRAAERGLPPITWPKGWPAQTWSLAPLRAQIMAEEQGLL
                     VPFALACYRKMFVEARSLAELNTIFDAARVAGLDPPEVRDRIAEPALKERLREYTDEA
                     TARGVVGVPTVAVGDELFWGDDRLEDAAAALARAG"
     gene            complement(7031..7747)
                     /locus_tag="VF517_14955"
     CDS             complement(7031..7747)
                     /locus_tag="VF517_14955"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX8054286.1"
                     /translation="MRGRGVAAAAALALLGAAPAAHADERVYAVPVNQYSSTVITIDQ
                     GEALFFRNIDLQQHDLVAKDQAAGKPLFGSPLLGSGEEALVVGAQQLKGGNYAFFCTV
                     HPFMTGTLTVTGAGAPAPGPSSGPADTTAPSVAVKVLDSSVSKVVKNGKLRVEVTDSE
                     PGNVALTATMRNGKKTVTIAKGARGFARAGKATVAMALTKPGRSALRGKRSAAVSVTA
                     RTADAAGNAGAGSGKRTLKR"
     gene            complement(7737..8135)
                     /locus_tag="VF517_14960"
     CDS             complement(7737..8135)
                     /locus_tag="VF517_14960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8054287.1"
                     /translation="MAESKGIDGVSGFFGMRWESAERIRLTIRPELINAGGLLSGAVA
                     YALVDYGMGSALWKQVDRENERIATINIAINYIQTARSGDIVCESTVDRRNRTVGVMR
                     SEVRHEDGRLLATAVGSFAIVRRRGPEDAR"
     gene            8204..8806
                     /locus_tag="VF517_14965"
     CDS             8204..8806
                     /locus_tag="VF517_14965"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8054288.1"
                     /translation="MGGALWDRIDERCERWNVLRHPFYTRWSEGELTSEELARYAGQY
                     RYAVQALADMTAAAAEQHDELHAHAAEEAAHVSLWDDFARATGNAGDDQPNLETRACM
                     ATWSAEDGLLPALARMYAIEKAQPEIARVKREGLVDLYGYEPGPATAYFELHEHLDVE
                     HADEVRGLIEELGGDADEHALLDAAESAVRANWRLLDGVS"
BASE COUNT         1325 a         3190 c         3212 g         1213 t
ORIGIN      
        1 tcgagggcgt gcgcttcgtg cgcacgttcg tggtgatcac cgccgcggag cgtccgccgc
       61 atcccgcggc gcggcgcttc ctcgacttcc tgaccgagtc gccacgaccg ccagccgggt
      121 gggctcgtcg cggccgcgga ggatctcctc ttcgccagcg agccagcggc cgcgctcctc
      181 gccgctcgcg cagtcgacgg cgctccccga cgccacgagc cgctcgggcc ggcgcttggc
      241 cagctcggag agccgcgccg cctcgttgac cgggtcgccg atcaccgtgt actcgaagcg
      301 ctcctcggcg ccgacgttcc ccgccaccgc ctcacctgcc gacacgccga tgccagcgtc
      361 cacgtccggc agctcgtcgg tgagccgcgc gcgcagctcg cgcgccgcgc acagcgccgc
      421 gcccgcgggg tcctcgcgcg cgaccggtgc gccgaacacg cacagcgcgc cgtcgccctc
      481 gaacttgttg accatcccgc cgtaggactc gaccacctcg acgaccagcg agaagaagcg
      541 gttgagcagg gccacgacct gctccggcgg cagccgcgtg gccatccacg tggagccgac
      601 taggtccacg agcagcacgc ccacctcgcg cacctcgccg ccgagcttca cgccgccgtc
      661 gagcgccgcg cgcgccacgt cgcggcccac gtggcggccg aacaggtcct ggagccgctc
      721 gcgctcggcc agcccggccg ccatgcggtt cagcccgacc tgaacgagcc cgatctcgct
      781 gctgtcgtcc acgggcacct gcacgtcgta gtcgccctcc tcgatgcgtc cgagcgcgct
      841 gcgcatgccc gcgagcgact cgctcagcga cgtcgccgtg agctttgtgg cgatgagccc
      901 gctgaagccc gcgaccatcg cgaggaacag gacgccgccg gcgagcagct cgccgtcgat
      961 gtcggcgaac aggcccgaga ctcccaccgc gctgagaccg agcagcggcg tgccggtggc
     1021 gagcagccag accatcagca ctcgcacgcc gacgccgggt ccgctcgggg ccaccggcgg
     1081 cgcggccgac agcgcgagcg tggtcaccgg ccgcagcatc cgctccgcgg cgaggtagtg
     1141 cacgccggag gtggtgatgc cgccgaggat cagcgtgagg cccacgatcg cgccgccgag
     1201 ggcgccggcg cccgccgcga tcccgcctac gacgttcagc gtgctgaaga tcaccgcccc
     1261 gaggatccag aagcgcgccg ccatcgccgc ctgcacgtag gcgatcgaca gcgcctgctc
     1321 gcgctcggcg ggagtcgggt cgcgcacgcc cacccagtcg cggagcgagt ccgaggcgcg
     1381 gtagccccag cgcgtgccca gcacgaaggt tgtcggcagg agcaccgcga acacgaccgc
     1441 gttcaccgcg atcgcgctgg tcgagtcgta gccgtcgaac gtcggcggca gcaggaacgt
     1501 gaggaagaag aaggtgtcga acgccccgag cccgttggcg aggatgccga ggcgattgag
     1561 ccggcggcgc gtgcgctgca cgagctccgt gccctcgtgt gcggtcgtga actggaccgg
     1621 acagtgcgcc gccgttgccc ccatgcgtgg attgtccccg acgcgccgtg ttccatgacg
     1681 cggctgtgac cgttgtcacg gtcggtcggt tggcgcggtc agtcgcggcc ggaatggcgc
     1741 gcttgcacta gcgcacgcgc acgttcgtca cgtacaccga cggcccacgg tccgagcgga
     1801 cccacgtctc gacgcggtac acgccgcgcg ccgccttgcg cttgccgatg cgcccgttcc
     1861 agcgcacggc atagccgcgc cccgagcgca ggcagcggtg cagcggctcg gccacgcgcc
     1921 tgcgcccgcg cgtgacccgc acctcaacct ccgccggctg cgcgagcacg agcttggcca
     1981 gcgtggggga ggtgccggcg cgcaggctgc gagcgcgctt gccgtagctc ttcaccatcg
     2041 ggcccggcgc cgagggcacc accacgcgct tgcccttcga ctcccacccc gacgtgatcc
     2101 gcacggtggt gcggcccggg cagccggcct tgaagcggcc gtcctgggtc tgggtggccg
     2161 ggtcgcggcg cgggtcgtcc gtgtcggacg gagccggcgc gagcacgtgc gggttgcctg
     2221 aagtccagat gcgcgccggg ttggggagcg cgtcgcgcgg cgtcgaggtc ggctcgctgc
     2281 gctgcgcgaa cgcgatgccg gtgaccgcga tcgcccccgc gagccccgga gccagggccc
     2341 gcgccacccg cacgcgcccg cgcgggcgcg tgaccaccgc gccgaacagc aggaaggcga
     2401 ccagcggcgc gccccagatc agcgcgcccg gcaggccacc cgcgcgcacg ccctcgcgcg
     2461 ggatcgggtc ggggtcgcgc agctcgagcg cgggcacctt ggcggggtcg ttgtagaccg
     2521 gctgcatccc gaacgcctcg aactggccgc ccgcgccggg ggcgagggtg tcgggcccgt
     2581 tcagcttcag ggagccgggc acgaagatcg gcacgctgtc ggtgatcacg ttgcggccgt
     2641 tcacgcgcac gagccggtag ccgtgccagt agccgtggtc ggtgccgacc gggccctccg
     2701 tgtagagctc gccgcccgcg ccgccgtcga tgtagtacgg cacgtcgctc gcgacgtact
     2761 ggaactggcc cttgatgtgg cccacgaaca cgccgtcgac ctcggtggtg gagacgatgc
     2821 gctcgaagtc ggcgatgtcc gaggccgtcg cgcccttgcc catcgtgtgg ttcgtggagg
     2881 tcgggtcgac gtagctctgg tcgcgcgggt cgcgcgtgcc catgtgcatg accacgaaca
     2941 cgacctgacc ggagcgcgtg ccctcgagcg cgcgcgactc gagccagtcg agctgcgtgc
     3001 gcgcgtcgcc gtcggccggg ttctgctggt tgttggagca gaacgtgatg tcccagcacg
     3061 agttgtcgac gaagatccag cgcacgtttc cgtagtccac ggagtagtgc gtggccgcgc
     3121 ccggcggatc gctctcgggc cgctcgtgca ccgtgaagct cggatcggcg tacggcttgg
     3181 cgtcgcccat cggccagggg cgctcggcga acacctcgcg gtagttggtg gtcgagccgc
     3241 cgggcgggaa gcccggctgg ttggccggtg cgttcaggcg gtcgtggtta ccgacgcccg
     3301 cgaagtaggg gatgcccgcc ttgtcgtagg cgctcatgat ctggcgccac atcgcgagct
     3361 ggtccaccgt gccgtcgttg gccttgtcgc ccgacatcgt cacgagcttc ggccggtagc
     3421 ggatggtgtc cgcgacgacc ttcggcagca gcgtcatccc gtcgtcgtcg ttccagtgct
     3481 cctcgccgta gtggacgaac aggtggccgg ggccctgggg cggcggccag gtctccggcg
     3541 ccgcgccggc ggccggagcg gccacggcga gcaccaccag ggcggcggcg agcgggtgga
     3601 gcagacgtga caccggagtc attatcctgc tttgcctcaa tcaggtaggg gggttaccag
     3661 gtggggaaga ggctcggcag ttccgcgctc gtctgcgcgg tcatcgctat gacgcttgcc
     3721 gcggatgccg cggccacgcc ggtctgcacc gacggctaca agggcggccc gcccgtctcg
     3781 cagtgcggcg gccggatctt ccccgaagcc catctcaccc agggttatgt ccagtacacc
     3841 ccgaaccctc agggcttcgc ggagtaccgc cacgggctcg agtacctggc gcagaagtac
     3901 ccgcgctgga tctccgtgtt cacgctgaag gaccgctacg ggaaggacgc cgtgagcgcc
     3961 ggaccggacg gcatccgccc gtacgagccc ggcgacaccg gcgacggccg cgacatcctc
     4021 gtggtgaaga tcaccgacca cgagacgccc gacgcgggca agcagccgct cacgttctcg
     4081 ctctcggtcc acggcaacga gcgcggcggc ctggagggcg gcgtgcgcac ggcggaggac
     4141 ctggcgctgg ccgcgacgaa cggcggcgca gtgcccgacg gcgtggccaa ctacgacacc
     4201 accacgggcc gcaagcccgc cttccacacc tacgaggtgc gcgagctgct gaagaaggag
     4261 gcggtctacc tcatcgactt caacctcgac ggctgggcga tcggcgactt cttcaacatc
     4321 cccactcagc cgtacgcgcg cggcaacagc aagggcaccg acatcaaccg ccagatgccc
     4381 acgctcggca gcatcaacac gacccgcaac ccgcttcagg agagcgaggc gcgctcgggc
     4441 ctccagttca tgaacgacgt ggcggcgagc ggccccggcg gcaagatggc ctacggcgcc
     4501 gacgtgcacg gcgagctgac ctcacaggcg tacgtcgatg tcatgtaccc ggcggggcag
     4561 ttcgactccg tcgaccaccg ccgcctgatg tcgatcgcag agcggaccaa gtcggtgatc
     4621 gacgcgaccc tctaccaggg gatcatcgac cagaccgaga acgccggcgg cggcaacgag
     4681 ggcgagccgg gcggcgacat cccgaccaag cccgcccact ggggcacggt ctgggacacg
     4741 ctcggctaca cggacaccgg gttcatcggc gactacatgg ccaccgacct cggcgtgacc
     4801 ggcatggact acgagatctt cctcaaccac acggtgccgg acaaggtctg gaacgtgtac
     4861 ctccaggaga accacatcaa cgccacgcgg gggatcatca agaccgcgat ggcctacgcg
     4921 cggacccagg agcaggagtt caacgcgcag aacgtgaagg tcgatccgaa ggggcgggtc
     4981 ggctacgtgg tcaaccccga cacggtcacc gacaaggacg agaacgggcc cggcacgctg
     5041 ccggggccga agaaggacgg caagggcgcc gacgggcagc cggtccagca gcgctcgtac
     5101 gacgtgacga accagcagtg gttcgccgac acgaacaagc tgatgccgca gtcgttcgcg
     5161 cgcttcgcct cgggcgacgt ggcgcgcgac gcgaaggcgc tgaacgcggt cgacacgctg
     5221 gtgctcgccg acgtgccgct gccgcaggac acgcgtgcgc gcaaggtcga cgatgccgcg
     5281 tactaccgca acgtgaagtc gtgggtcgag tcgggcggga acctcgtgct gaccgaccgc
     5341 gcggtgcacg ctctccacgc gatgcgcgtg gtgcctcagg aggccgtgca ggacgtcagg
     5401 gtctaccagc cgtacgcgaa catcagcgac ttccagcact cgctggtgga ggggctgcgg
     5461 ccgaacgcgc ggcagctcgt ggaggcggcg atcctgggtt acgggatcgg caacaacgcg
     5521 tcgccgatga cgatcgtgga ccgcgcggcg tgggaggccg cgggcggcaa gacggtcgcc
     5581 acgaccggca acaacagcgg cgacagcgac gacggcacgc gcgcctcgat cggcgagctg
     5641 aagctcggca agggcctgat ccggatcatc ggcggcgcgc tcccgatgcc caccgaggag
     5701 aacgaccacc gctacggcct gcgcgactac ggcccgacct acagcggact gttcgtgctc
     5761 gagaacagcc tgcgccatga cgtgccggag ctcggccgca cggcgggcgc cgcggcctgc
     5821 atcaagggct cgtcgcggac ggtgccgatc tcgcgcaagg tgcgcggcga gcgcgtgcgc
     5881 tccacgcggg tgaacgcgaa cgggccgacg cggctcacac gccgcaatgg ccggctctac
     5941 gcgatcgtcg acatccgcaa gctgaagggc aagacgctcg tgctgcggat ctcgcggcgg
     6001 acggcgtccg gcaagctggt gaagacgaag cacacgcggc tggtctgccg ctaggcgcag
     6061 gcgcaggcgc aggcgccggc gggcggcgcg ctcagacggg gcagtcgacg acttcttcgc
     6121 gctggccctt gacctgaagc gcgcggaacg tgccgttgcg ggtcccgacc tggaactgcc
     6181 acttgacctc gtcgtcctgg taggcgacga ctcggtcgcc gaagaagtcc tgcgatgcgt
     6241 cgggcttgcc gaagcggtcc tcgagctgct cgagcgtgag cttctccttc acggccctgc
     6301 atgcctcggc ctcgctgaga ggcgtcggac cgtcgtcctc cgcgcagccc gtgactgcga
     6361 gggcggccag gagggtgagc agcaaccgac ggcgaggcac gcggaccggt acgttaacca
     6421 cgtgggagaa gcgccgatct tctactacga cacgaactcc ccgtacgcgt acctcgcggc
     6481 ggagcggatc caggacgtga tcacggaggc cgaatggcgg ccgatcgcgt tcggcatgct
     6541 gctgcgcgac atcgaccgcg tgccgtggtc gctgaggccc ggccgcgagg agcagatggc
     6601 cgactgcgag cggcgcgccg ccgagcgcgg gctgccgccg atcacgtggc cgaaggggtg
     6661 gcccgcccag acctggtcgc tcgcgccgct gcgcgcgcag atcatggccg aggagcaggg
     6721 gctgctcgtg ccgttcgcgc tcgcctgtta ccggaagatg ttcgtcgagg cgcgctcgct
     6781 ggcggagctc aacacgatct tcgacgcggc tcgcgtggcc ggcctcgatc cgcccgaggt
     6841 gcgcgaccgg atcgccgagc cggcgctgaa ggagcgcctc cgcgagtaca ccgacgaggc
     6901 gaccgcgcgc ggcgtggtgg gcgtgccgac ggtggccgtc ggcgacgagc tcttctgggg
     6961 cgacgatcgc ctcgaggacg ccgccgccgc gctggccagg gcgggctaga gccggccggc
     7021 gcggggctag ctagcgcttc agcgtgcgct tgccgctgcc ggcgccggca ttgccggcgg
     7081 cgtccgcggt ccgcgcggtc accgacacgg ccgccgagcg cttgccccgc agcgccgagc
     7141 ggccgggctt ggtgagcgcc atcgcaacgg tcgccttgcc cgcccgcgcg aagccgcgcg
     7201 cgcccttcgc gatcgtcacg gtcttcttgc cgttgcgcat cgtggcggtg agggccacgt
     7261 tgccgggctc gctgtcggtg acctccacgc gcagcttccc gttcttcacg accttggaga
     7321 cgctcgagtc gagcaccttc acagcgacgc tcggcgccgt ggtgtcggcg ggtcccgacg
     7381 acggtcccgg agcgggcgcg ccggcgccgg tcacggtgag cgtgccggtc atgaacgggt
     7441 gcaccgtgca gaagaacgcg tagttgccgc ccttgagctg ctgcgcgccg acgaccagcg
     7501 cctcctcgcc gctgccgagc agcggggagc cgaacagcgg cttgccggcg gcctggtcct
     7561 tggccacgag gtcgtgctgc tggaggtcga tgttgcggaa gaagagcgcc tcgccctggt
     7621 cgatcgtgat gaccgtgctc gagtactggt tgaccggcac ggcgtagacg cgctcgtccg
     7681 cgtgtgcggc gggtgcggcg cccagcagcg cgagcgcggc cgcagccgcg actcccctac
     7741 cgcgcatcct caggcccccg tctccgcacg atggcgaatg atcccacagc ggttgccagg
     7801 aggcgtccgt cctcgtggcg gacctcgctg cgcatcacgc cgaccgtgcg gttgcggcgg
     7861 tcgaccgtgc tctcgcagac gatgtcgccc gatcgagcgg tctggatgta gttgatggcg
     7921 atgttgatgg tcgcgatgcg ctcgttctcg cggtcgacct gcttccagag cgccgagccc
     7981 atgccgtagt cgacgagcgc gtaggccacg gcgccggaga gcagtccgcc ggcgttgatg
     8041 agctcgggac ggatggtcag ccggatgcgc tcggcgctct cccagcgcat cccgaaaaac
     8101 ccgctgactc cgtcgatccc cttgctctcg gccacgggcg ggcaggctac agcggttatg
     8161 gttcccgccc tctgagcatg ccgggctaac gagggaggcg gctgtgggcg gtgcgctgtg
     8221 ggaccggatc gacgagcgct gcgagcgctg gaacgtgctg cgccatccct tctacacccg
     8281 ctggtcggag ggtgagctga cgagcgagga gctcgcgcgc tacgcgggcc agtaccgcta
     8341 cgccgtgcag gcgctggccg acatgaccgc ggccgcggcc gagcagcacg acgagctgca
     8401 cgcgcacgcc gccgaggagg ccgcgcacgt ctcgctgtgg gacgacttcg cgcgcgccac
     8461 cggcaacgcc ggcgacgacc agcccaacct cgagacgcgt gcctgcatgg ccacctggtc
     8521 ggccgaggac gggctgctgc ccgcgctcgc gcgcatgtac gcgatcgaga aggcccagcc
     8581 ggagatcgcg cgcgtcaagc gcgaggggct cgtggacctc tatggctacg agccgggtcc
     8641 cgcgaccgcg tacttcgagc tgcacgagca cctcgacgtg gagcacgccg acgaggttcg
     8701 cgggctgatc gaggagctgg ggggcgacgc cgacgagcac gccctcctcg acgccgccga
     8761 gtccgccgtc cgggcgaact ggcggctgct cgacggcgtc agctagcccg ggtcagtcca
     8821 ccgccacgtg cgcagggccg cgacgaacgc gcggcggtcg cctgcatagc gctgctcggc
     8881 gctcgacagg gtgatcctgt acgcctgctc gccgtggacg gccacgaact cgagttggcc
//
LOCUS       DASVPL010000269         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_72166, whole genome shotgun sequence.
ACCESSION   DASVPL010000269 DASVPL010000000
VERSION     DASVPL010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72166"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VF517_14970"
     CDS             complement(<1..669)
                     /locus_tag="VF517_14970"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8054289.1"
                     /translation="MKHPLPIAPELGTRDGLAYALFAPEGEPRAGILILHGAGSAKES
                     HFDFARTAVAHDMVAVAFDQRGHGRSEGSFSSSAVEDALAMCALVRERAPRLALRGSS
                     MGGFMAILAAAADGDADAVVGICPSPEDFLLRGLRADRFEFRADRESLEPWLEAVSLR
                     NAAASLAPRCALLLMHAQGDEQVPYTMSQELFEAAGEPKRLLLMPGGHHRSIQHDLEL
                     QTEAL"
     gene            684..1943
                     /gene="lysA"
                     /locus_tag="VF517_14975"
     CDS             684..1943
                     /gene="lysA"
                     /locus_tag="VF517_14975"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX8054290.1"
                     /translation="MASLEERGGVGAGVSHVYPLGSRINEQGRLEIGGCDVVELAERY
                     GTPAYVYAEDDLRARARAYVDAFRAHTDRFEIVYASKAFPCTAAYRLLAEEGLACDVA
                     SGGELYLALRGGFAPEKIYFHGNNKSEAELRQAHEAGVGTVVVDSFDELELLERVAPG
                     QPIMLRVTPGITPTTHSYITTGQVDSKFGFGLDDMPRAIESVGRLELRGLHAHLGSQI
                     FDLEPFERLGEVLTEIGDYPLLNLGGGLGIAYTREQLPPSIEEYVETLVRSAPDGVTI
                     LCEPGRSIVGNAGVTIYRVGTVKRIPDIRTYVAVDGGMSDNLRPMLYGAQYEAEVADR
                     FGGEDVCTIAGKHCESGDVLIRDVKLDDPHVGDVLVTPATGAYGHAMANNYNGIPRPP
                     VIFCKDGEARVVVRREKLEDLAARDAG"
     gene            1947..>2553
                     /locus_tag="VF517_14980"
     CDS             1947..>2553
                     /locus_tag="VF517_14980"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX8054291.1"
                     /translation="MTPFRVGLLGHGTVGAAFQELLEERAGAIESVTGQRPEVAGVLT
                     RSRGDFDEIVAASDLVVELIGGVEPARDYILGAVRAGRHVVTANKAVLSQHGEEIYDA
                     ARESGVQLRFEAAVGGVVPVIRVIHETLAAAHVDRVHGIVNGTTNFILTEMTRTGATY
                     DEALAEAQRLGFAEADPTEDVSGRDAAAKMAIIARLAFNAPV"
BASE COUNT          372 a          907 c          937 g          337 t
ORIGIN      
        1 cagcgcctcg gtctgaagct ccaggtcgtg ctggatcgag cggtggtggc cgcccggcat
       61 cagcagcagc cgcttcggct ccccggccgc ctcgaacagc tcctgcgaca tcgtgtaagg
      121 cacctgctcg tcgccctggg cgtgcatgag cagcagtgcg cagcgcggcg ccagcgaggc
      181 ggctgcgtta cggagtgaca cggcctcgag ccacggctcg agcgactcgc ggtcggcgcg
      241 gaactcgaag cggtccgcac gcagcccgcg tagcaggaag tcctccggcg acgggcagat
      301 cccgaccacg gcgtccgcgt cgccgtcggc ggcggcggcg aggatcgcca taaagccgcc
      361 catgctcgat ccccgcagcg cgagccgcgg tgcgcgctcc ctgacgagcg cgcacatggc
      421 gagcgcgtcc tcgaccgccg aggagctgaa ggagccctct gagcgcccgt gcccgcgctg
      481 gtcgaacgcc accgcgacca tgtcgtgcgc caccgcggtc cgggcgaagt cgaagtggct
      541 ctccttggcc gagccggccc cgtgcaggat caggatcccc gcccgcggct cgccctcggg
      601 cgcgaacagc gcgtaggcga ggccgtcgcg cgtcccgagc tccggggcga tggggagggg
      661 gtgcttcata ctctgaggcg attatggcct cgctagaaga gcgcgggggc gtgggcgccg
      721 gcgtgtccca cgtgtacccg ctgggttcgc gcatcaacga gcagggacgg ctcgagatcg
      781 gcggctgcga cgtggtcgag ctggccgagc gctacggcac gcccgcgtac gtgtacgccg
      841 aggacgacct ccgcgcgcgg gcgcgcgcgt acgtggacgc cttcagggcc cacaccgacc
      901 gcttcgagat cgtctacgcc agcaaggcgt tcccgtgcac cgcggcctac cggctgctcg
      961 ctgaggaggg gctcgcctgc gacgtggcct ccggcgggga gctctacctc gcgctgcgtg
     1021 gcggcttcgc gcccgagaag atctacttcc acggcaacaa caagagcgag gccgagctgc
     1081 gccaggccca cgaggccggg gtcgggacgg tcgtggtgga ctcgttcgac gagctcgagc
     1141 tgctcgagcg tgtcgcgccg ggccagccga tcatgctgcg cgtgacgccc ggcatcaccc
     1201 cgaccacgca ctcgtacatc accaccggcc aggtcgactc caagttcggc ttcgggctcg
     1261 acgacatgcc gcgggcgatc gagagcgtcg gccggctcga gctgcgcggg ctgcacgccc
     1321 acctcggctc gcagatcttc gacctcgagc cgttcgagcg gcttggcgag gtgctcaccg
     1381 agatcggcga ctacccgctg ctgaacctcg gcggcgggct cggcatcgcg tacacccgcg
     1441 agcagctccc gccctcgatc gaggagtacg tggagaccct ggtgcgctcg gcgccggacg
     1501 gggtcacgat cctctgcgag ccggggcggt cgatcgtggg caacgcgggc gtgacgatct
     1561 accgcgtcgg caccgtcaag cggatcccgg acatccgcac ctacgtggcc gtggacggcg
     1621 gcatgtccga caacctgcgg ccgatgctct acggcgccca gtacgaggcc gaggtggccg
     1681 accgcttcgg cggcgaggac gtgtgcacga tcgccggcaa gcactgcgag tcgggcgacg
     1741 tgctcatccg cgacgtgaag ctggacgacc cgcacgtcgg cgacgtcctg gtcacgcccg
     1801 cgaccggcgc gtacggccac gcgatggcca acaactacaa cggcatcccg cggccgccgg
     1861 tgatcttctg caaggacggc gaggcgcggg tggtcgtgag gcgcgagaag ctcgaggacc
     1921 tggcggcgcg tgacgccggc tagcgcgtga cccccttcag ggtcggcctg ctgggccacg
     1981 ggaccgtcgg ggccgcattc caggagctgc tggaggagcg cgccggcgcg atcgagtcgg
     2041 tgaccgggca gcggcccgag gtcgccgggg tgctgacgcg ctcgcgcggg gacttcgacg
     2101 agatcgtcgc ggccagcgac ctcgtggtcg agctgatcgg cggcgtcgag cccgcgcgcg
     2161 actacatcct cggcgccgtg cgcgccggca ggcacgtggt gaccgcgaac aaggcggtgc
     2221 tctcgcagca cggggaggag atctacgacg ccgcgcgcga gtccggcgtg cagctgcgct
     2281 tcgaggccgc cgtcggcggg gtcgtgccgg tcatccgcgt gatccacgag acgctcgccg
     2341 ccgcccacgt ggaccgcgtg cacgggatcg tgaacggcac caccaacttc atcctcaccg
     2401 agatgacccg caccggggcc acctacgacg aggcgctggc cgaggcccag cggctgggct
     2461 tcgcggaggc ggacccgacc gaggacgtct ccggccgcga cgccgccgcg aagatggcga
     2521 tcatcgcgcg gctggcgttc aacgcgccgg tgc
//
LOCUS       DASVPL010000270        14038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_110670, whole genome shotgun sequence.
ACCESSION   DASVPL010000270 DASVPL010000000
VERSION     DASVPL010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110670"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VF517_14985"
     CDS             complement(<1..546)
                     /locus_tag="VF517_14985"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX8054292.1"
                     /translation="MGARGLRRRDLLRDGAVAAGGVALAPALARLGPLAERAAAAPPA
                     HKGPGPYGPLQASNLGDGVQVPASFSVREIARGNVPVPGTTYRWHVFSDGSGSYALPD
                     GGWLFASNAESPEPGEAFGGASVIRFDRDGNARDAYRILSGTRSNCGGGITPWGTWIS
                     GEEVDTGLVWEADLFGKKEAVP"
     gene            complement(566..638)
                     /locus_tag="VF517_14990"
     tRNA            complement(566..638)
                     /locus_tag="VF517_14990"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(603..605),aa:Arg,seq:cct)
     gene            676..2136
                     /locus_tag="VF517_14995"
     CDS             676..2136
                     /locus_tag="VF517_14995"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8054293.1"
                     /translation="MRPRLQRLRQALALLGVVLATLGGALAAFSAYTAERDISVGTVR
                     LSIEPFDHGALDLYAPIVDWGVRFPVVRMPARVKVDIRSVDRQAVAKVAEGGSVDVRE
                     LRTDARDAIASYLRELIAIVFAAGLLVGALAAFALRSRTAVRWPWLLLTAAVTATAIA
                     VALVVLLPPRGELTEPEYYAHGSDIPRALEAIEAATRSPSVLSEELDAQLVGLARLVV
                     SPSGRPAPAGLPRLTLASDLHNNVLALPTLERAARDGPLLFAGDLTDQGRPFEVALVR
                     RVVAAGRPFLFVSGNHDSDTLVRRLVAEGAIVLTERGRLEPGGSYGPVVFRFGGLRVA
                     GYSDPNPRRRADGYRGPPNPGPTPAQKVAFADWLDGLRGEVDVVMAHAPALLETALER
                     LRRRPPDRPLVLLVGHTHVPNAERIANNVVLVNGGTAGAGGSGNLAEGQRIGLAVVTY
                     QPGPAFEPVAADLVEVDPGDGSARAERHPLGGPPEG"
     gene            complement(2184..2411)
                     /locus_tag="VF517_15000"
     CDS             complement(2184..2411)
                     /locus_tag="VF517_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054294.1"
                     /translation="MTDELDLPLPAESAPAADPVENVASSAEIADSSAEVTVVQEAAT
                     SEAVANTTAEMRVVDLSQDYSAVVFDADLED"
     gene            complement(2404..2562)
                     /locus_tag="VF517_15005"
     CDS             complement(2404..2562)
                     /locus_tag="VF517_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054295.1"
                     /translation="MRIESSERVVEASAEVSDAGDSTSQAVAETTAHLHFEGKGFDPE
                     LEGRYEDD"
     gene            complement(2567..3103)
                     /locus_tag="VF517_15010"
     CDS             complement(2567..3103)
                     /locus_tag="VF517_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054296.1"
                     /translation="MALDYTRLDHKTVAWATENARELVKEEADRLRALDAKSAQLVGF
                     AGVILAVLGSLGKDAFLRDLGYVGDPLFAAAYFLSMLVLTASILWLVFFTLKPQRLVV
                     IDAKELSAYLEDERLLQAEAAALQIRTLRALRDAASWAQRVGKQKASRLSTGMALFAI
                     GLAAALVAVTTMGAGSLG"
     gene            complement(3470..4087)
                     /locus_tag="VF517_15015"
     CDS             complement(3470..4087)
                     /locus_tag="VF517_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054297.1"
                     /translation="MPPNERPIPRFIAEPPQEGYPYGRWAEALTERFLDAARALDSED
                     EHGDPGEVIWFPERTYGGRIYIPATSATTQGFELFGYVSYTREHEGAEARDFQAVADY
                     TDETAEANPDWRLDLSDWEIGHWRGPQGRRGEITLVWGVALEHKGLVVTTELGPTTTD
                     QCALVEDRFTLVSLDNYTGDYVEVVLWGSDGSRIAGESLYEDDEG"
     gene            complement(4100..5116)
                     /locus_tag="VF517_15020"
     CDS             complement(4100..5116)
                     /locus_tag="VF517_15020"
                     /inference="COORDINATES: protein motif:HMM:NF027593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HEX8054298.1"
                     /translation="MPELRIDPLTGLRTIVAAERSTRPGGELRVEPQPPFDPETDPFL
                     EGHEDRTPPELFALRPGGGPPDSPGWRVRVVPNLYPSLAGPSGGDGDAPADPLAAGRG
                     EPQLFAARAATGAHEVIVNTPRAVGSLHDLEPEELEIAMEAWRERMRANSDAACLHLI
                     VNEGRAAGASLPHSHAQLYGLAFVPAAVARERERFTAHSDRTQGRNLLEDLLQEEVRR
                     RERIVVIDREAVAICPFASRTPYHVQLVPRTPRARFEDDGPLGARLLHDVLGRLAGVL
                     GAVPPFNLWVRTAPRGADRFCWRIDLVPRLTNLAGLELGTGLNLNVVAPERAAEQLRG
                     AGDR"
     gene            complement(5109..5534)
                     /locus_tag="VF517_15025"
     CDS             complement(5109..5534)
                     /locus_tag="VF517_15025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8054299.1"
                     /translation="MTTTVTDDPGLVEQALALRERVFCGEQGVALEAERDGHDDEAIH
                     VVCLRTGRVLGTCRVLVRDGVARLGRMAVEPLARGRGIGGEVLAEAERAARVAGATRM
                     RLHAQSAAIGLYERAGYEQVGDPFVEEGIDHVTMERALA"
     gene            complement(5545..6093)
                     /locus_tag="VF517_15030"
     CDS             complement(5545..6093)
                     /locus_tag="VF517_15030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8054300.1"
                     /translation="MRRLVPLLLAAALLAGCNDDPESAAPSRAEAQRQLAGAPAPLAS
                     LHREANRLLPGGAPAFERRLRELRGHPVVINKWASWCPPCRAEFPHFQRLSVELGREV
                     AFVGVDSEDNREDAREFLGDFPVSFPSYEDPDQKVARVFKGHVNFPTTAFYDRSGKLT
                     FIHQGGYATEARLREDIERYAR"
     gene            6310..8874
                     /locus_tag="VF517_15035"
     CDS             6310..8874
                     /locus_tag="VF517_15035"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX8054301.1"
                     /translation="MADSKTLTAASPVAEEDLARVEDFTREPFVHEDEDPKVVLAAGQ
                     PRRRAFDAAVAEIEAGHENPSIAWQQEFALMLGLERLMSQDEPHLADGTLLSAHQVDA
                     LSGTLIALLAEVQSSGRRNGGGNGAVAAEALSELPSGEVELEGDEDIGDEDEPLDWDP
                     DAEAEDEIEDDAPPSDDPGAARRFWFEHATGAGKTVAALGFVEATRTGGVLILTHRRN
                     LVDQFLGELKDRGYKKLISPPLLDGKGEDKAKAPVTVETYQWYVRNAGRISGAYSVVI
                     CDEAHTALGEKTSASIRSWQGPIFVGMTATGALIARHVTDLFPTQTSRFDLAQAARRG
                     VIAPLRCVRIPPGVGVRTIAKVPLRKGEVDQDFDQEELAELLDQAPFNSAIADLYRVR
                     FKDLPGVVYSAGVRHAHNVAKAFVDAGLKAKAVSGETPKRELTRVLAAYERGEIDVLV
                     NAQLLAEGWNSPRATVCMHLAPTASKRIYQQRVGRVTRRNPGKEAGIVVDFVHPATTH
                     DDPVVTLHSLLDRDVYRGGAIVVGPVRRGRGRRLRVERRVLPVTPDPDRRAAVFEREL
                     WRIAVEHLSWQEQHVWAALAGARVASNNWRRAKAMLHFDSTGELRRRFVITCLQRNRN
                     TQLRIRALQEIAAARDAEAFDEAVEIIGGWSRDERREGVKVLLQALAERPIGRRDQAT
                     AWLWRLAEYTREVHEEYAVQRWPETKRLLGLLVNSSGGAHARNARRLVQAARKHDRRL
                     SAALLAAALAHTPEAEEVLRGARTRMARKPSALARELLRNFPKGRRRRGGRRRKRSGG
                     GEEKAGSADEAAVQNAQNGGGEEAEETEADEVEPQADDSSSDAASSDDDDEPAEAA"
     gene            complement(8910..9602)
                     /locus_tag="VF517_15040"
     CDS             complement(8910..9602)
                     /locus_tag="VF517_15040"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX8054302.1"
                     /translation="MIGTPRVHHRVTDSTNQRAKELANAGAPHGTTVTADEQTAGRGR
                     QGRSWVAPPRSAVLLSVVLRDLDESAALLPLATAVAVCEAAESLAPVRCEIKWPNDVW
                     VARRKLCGILVEGRPAAGWAVVGLGVNVTTREFPADLVATSLALSGSSASVDDTASAV
                     LAMLERWLSSPSAEVLDAWRARDALLGSNIGWGEGEGVAAGIDDRGSLLVDTSEGRVA
                     LGAGEVHLRLNS"
     gene            complement(9599..10339)
                     /locus_tag="VF517_15045"
     CDS             complement(9599..10339)
                     /locus_tag="VF517_15045"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX8054303.1"
                     /translation="MIRRIAPDVPIVDIAHGIERHAVRHGALVLAAALPYMPVGVHVA
                     IVDPQVGTERRALALRCADDRLLVGPDNGVLSLAWQRSGGVVEAVDVSRSPHRLEPVS
                     ASFHGRDVFAPVAAHLAAGAELADAGDPLDPGDLVALDLPSARLEGGAVVAHALAVDG
                     FGNVALDLRHEHLLELDLVIGSRALVHARGGRREATVVQTFADVPAGEFLIYEDAWGA
                     VALAVNRGNAAMELRLQPDDELRITPAP"
     gene            complement(10393..11874)
                     /gene="cobA"
                     /locus_tag="VF517_15050"
     CDS             complement(10393..11874)
                     /gene="cobA"
                     /locus_tag="VF517_15050"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX8054304.1"
                     /translation="MAGKAYLIGAGPGDPGLITVRARELLAAADVVLYDRLVPPAVLD
                     GLRAELVYVGKEPGGAAMPQDEINARLVEHTRAGRMVARLKGGDPFVFGRGGEEAEAL
                     AGAGLEFEVVPGVTAGVAAPAYAGIPVTHRDGASAVAFVTGHEDPTKPESVIDWDALA
                     AFPGTLVFYMGVRNLERIAWSLVEAGRSEGEPAAVVERGTTARQRVVTGTLGDIAARV
                     AESGVKPPALTVVGAVADLRERLAWFEAGRPLLGRSVVVTRARDQASALAARLAGLGA
                     EVVEAPVIRTEPLPSVELPPIDGYDLICLTSQNVIEPFFDALRRQGTDARALAGARVA
                     TIGTATAAALAARGIDPDVVPERQMAEGLVAALAGEEVDGRRVLFARAEEGRDVVPDA
                     LRERGAAVDVVALYRTVADSPAAPTLEAAARADFVTFTSSSTVRYFLEAAGDAFPRGA
                     RVISIGPATSATARELGLEVHVEAERHDVDGLVDALLEDARSS"
     gene            11987..12568
                     /locus_tag="VF517_15055"
     CDS             11987..12568
                     /locus_tag="VF517_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054305.1"
                     /translation="MKRIAGLAAALAVASLVSAAPVLAAPATIGSTGMTYANGGISCI
                     SALQGATAPGSPAYVVPFDGTIASWSVQGANQPATARLQVYRPLGPNEWRLVAESAVR
                     QVSATGVTTSAARIAVRQGDIIGRTGLGCVYTSALSADDVRTFGYHPTVGSVATPDGP
                     GASAWRLNLRATVEPDCSKGNRKKPKVCRAARR"
     gene            12664..14007
                     /locus_tag="VF517_15060"
     CDS             12664..14007
                     /locus_tag="VF517_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8054306.1"
                     /translation="MEAGRHLAWGYVDQGPLTPLLARAMTELSEGSLTVLRVPSALMC
                     AGMVLLTGMLARELGGTRRAELIAAGCTAVGVVFLTTGHLLSTTTFDLLVWIAVTYLA
                     VRAVRRGEERLWPVAGVVLGLGLLNKPLPAFLAAGLLAGVAIAGPRRLIRSPWVWAGA
                     AIALVMWMPWLVWQADHGWPQAEVSRAIAEGGSTSSEPRWAIVPFQFLLVSPPLAPVW
                     IAGLVRLFRDPVLRDLRFFAWTWVALAIAFMATGGKPYYLAGLLPLLLAAGAGAVDGW
                     LERGRRGARRVALGAAFALSVAIGVPIALPVLPVEDIEPVLAANDDVGNTVGWPGFVD
                     QVAAVYRRVPGAVILTSNYGQAGAIDRYGPDLRLPRAYSGHNAYHEWGPPPDRGGLVI
                     VIGEVEREGFRGCTLAHRIETVADLDTEERGTEVWTCRGTARPWSELWPELERFG"
BASE COUNT         1919 a         5138 c         5124 g         1857 t
ORIGIN      
        1 cggcacggcc tccttcttcc cgaacaggtc ggcctcccac acgagcccgg tgtcgacctc
       61 ctcgccggag atccaggtgc cccacggcgt gatccctccg ccgcagttcg aacgggtgcc
      121 ggacaggatc cggtaggcgt cccgcgcgtt gccgtcgcgg tcgaagcgga tcacgctcgc
      181 gccgccgaac gcctcgcccg gctcaggcga ctcggcgttc gatgcgaaca gccagccgcc
      241 gtcggggagc gcgtacgagc cggagccatc ggagaacacg tgccaccgat acgtggtgcc
      301 gggcacgggc acgttcccgc gcgcgatctc gcgcacgctg aagctggccg gcacctggac
      361 gccgtcgccg aggttcgagg cctggagggg gccgtacggc ccggggccct tgtgcgccgg
      421 cggcgccgcg gcggcgcgct cggcgagcgg gccaaggcgc gcgagagccg gcgcgagagc
      481 gaccccgccg gcggcgaccg cgccgtcgcg taggagatcc cgcctgcgca gcccccgtgc
      541 tcccatggcg ggcagcctaa ccgagcgccc cgggctggat tcgaaccagc ggcctaccgc
      601 ttaggaggcg gtcgctctat cccctgagct accggggccg cgggcggagg ttagtcgggc
      661 ggtagcgtcc gggccatgcg cccgcgcctc cagcgcctgc ggcaggccct ggcactgctg
      721 ggcgtcgtgc tggccaccct cggcggcgcg ctcgcggcgt tctccgccta caccgccgag
      781 cgcgacatct cggtcggcac ggtgcggctc tcgatcgagc cgttcgacca cggtgccctc
      841 gacctgtacg cgccgatcgt cgactggggc gtgcgctttc cggtcgtgcg catgcccgcg
      901 cgggtgaagg tggacatccg cagcgtcgac cgccaggcgg tcgcgaaggt cgcggagggc
      961 gggagcgtcg acgtgcggga gctgcgcacc gacgcgcgcg acgcgatcgc gtcgtacctg
     1021 cgcgagctga tcgcgatcgt gttcgccgcg gggctcctcg tcggcgcgct cgcggcgttc
     1081 gcgctgcgct cgcgcaccgc ggtccgctgg ccgtggcttc tgctcaccgc agccgtaacc
     1141 gccacggcga tcgcggtcgc gctggtcgtg ctgctcccgc cgcgcggcga gctcaccgag
     1201 cccgagtact acgcgcacgg ctccgacatc ccgcgcgcgc tcgaggcgat cgaggcggcc
     1261 acccgctcgc cgtcggtgct ctccgaggag ctcgacgcgc agctcgtggg cctcgcccgc
     1321 ctggtcgtgt ccccgtccgg ccggcccgcg ccggccgggc tgccccggct gacgctcgcg
     1381 tcggacctcc acaacaacgt gctcgcgctg cccacgctcg agcgcgccgc gcgcgacggg
     1441 ccgctgctgt tcgccggtga cctgaccgat cagggccggc cgttcgaggt cgcgctggtg
     1501 cggcgcgtgg tcgccgccgg ccgcccgttc ctgttcgtgt ccggcaacca cgactccgac
     1561 acgctggtgc ggcggctcgt cgccgagggc gccatcgtgc tcaccgagcg cgggcggctg
     1621 gagccgggcg gcagctacgg cccagtcgtg ttccggttcg gcgggctgcg cgtggccggc
     1681 tactccgacc cgaacccgcg ccgccgcgcc gacggctacc gcggcccgcc gaacccgggc
     1741 cccacgcccg cccagaaggt ggcgttcgcc gactggctcg atggcctgcg cggcgaggtg
     1801 gacgtggtga tggcccatgc gcccgcactg ctcgagacgg cgctcgagcg cctgcgccgc
     1861 aggccgccgg accgtccgct cgtgctcctc gtcgggcaca cgcacgtgcc gaacgccgag
     1921 cgcatcgcca acaacgtcgt gctcgtcaac ggcggcacgg cgggggccgg cggcagcggc
     1981 aacctggccg agggccagcg gatcgggctc gccgtggtga cctaccagcc cggtcccgcc
     2041 ttcgagcccg tggccgccga cctggtcgag gtggacccgg gcgacggctc ggcccgcgcc
     2101 gagcgccacc cgctcggcgg cccacccgag ggctagccgc gtagcacgtc gtcacgagca
     2161 agccgtcgcg tggtggacgc gatctagtct tctaagtcag cgtcgaagac aaccgcagag
     2221 tagtcctggc tgaggtccac aacacgcatc tcagcggtcg tgttggcgac agcctcagac
     2281 gttgcggcct cttgaaccac ggtgacctcg gcggaagagt cagcaatctc agcagaagag
     2341 gctacgttct ctactgggtc cgctgcgggt gcggattccg cggggagagg aagatcgagc
     2401 tcgtcagtca tcctcatacc ggccttccag ttccgggtcg aaacccttgc cctcaaagtg
     2461 cagatgcgcc gttgtctcgg caacagcctg cgaggttgaa tcgccagcat cggatacctc
     2521 cgctgacgcc tccaccacgc gctcgctgga ttcaattctc attgactcat ccaaggctgc
     2581 cagctcccat tgtagtcacc gcgactaagg cggctgcgag gccgatggcg aacaacgcca
     2641 tgcccgtcga gagccggctt gccttttgtt ttcccactcg ctgagcccac gatgcagcgt
     2701 cgcggagggc ccgcaaagtg cgtatctgaa gggccgcagc ctccgcctgt agcaaccgtt
     2761 cgtcctccag gtatgcagac aactccttcg catcgatgac gaccagccgt tggggcttga
     2821 gcgtgaagaa gacgagccag aggatcgacg cggtgaggac cagcatggag aggaagtacg
     2881 ccgcagcgaa gagcggatct ccgacatacc cgagatcgcg gagaaacgcg tcctttccga
     2941 gcgagccgag taccgcgagg atgacccccg cgaagccgac gagctgtgcg gacttcgcgt
     3001 cgagcgcacg tagcctgtcg gcttcttcct tgacgagttc ccgcgcattc tccgttgccc
     3061 atgcaacggt cttatggtcc aggcgcgtgt agtccaatgc catcactcaa atgatgccgc
     3121 gcggtgggga cggcaaggga gatccgccta cgggggcccg gctagccgcg gcggtaaaga
     3181 ccgcgtggtg agcggcgcga acacggcgga ggtcagcgcc gccgccccga gcgcgatcgc
     3241 gatctcactc gtcaccggct cgcgcgccac ccgcgcgacg cggtcgcgag gatcgagatc
     3301 gggttcacgt caacaacgcc tcgagcgcgc tcagcagcgt ttccggctcc acgaaaaacg
     3361 gtcagccgaa agatccccat gaagcccgcg ttcgtcaccg cgttcggcct cggtttcccc
     3421 gaaggacttg gcgaggccag tcgcctcgat cgcgaggctg cgctgcccgc taaccctcgt
     3481 cgtcctcgta gagcgactcg cccgcgatcc ggctgccgtc ggacccccag agcaccacct
     3541 cgacgtagtc gcccgtgtag ttgtcgagcg acaccagcgt gaagcgatcc tcgaccagcg
     3601 cgcactggtc ggtggtggtc gggccgagct ccgtggtgac cacgaggccc ttgtgctcga
     3661 gcgccacgcc ccagacgagc gtgatctccc cgcgcctgcc ctgcgggccg cgccagtggc
     3721 cgatctccca gtcgctcagg tcgagccgcc agtccgggtt ggcctccgcc gtctcgtcgg
     3781 tgtagtcggc cacggcctgg aagtcgcgcg cctcggcgcc ctcgtgctcg cgcgtgtacg
     3841 agacgtagcc gaacagctcg aagccctgcg tggtggccga ggtggccggg atgtagatgc
     3901 ggccgccgta ggtgcgctcc gggaaccaga tcacctcgcc cgggtcgccg tgctcgtcct
     3961 cggagtcgag cgcgcgggcg gcgtcgagga agcgctcggt gagggcctcg gcccagcggc
     4021 cgtacgggta gccctcctgc gggggctcgg cgatgaagcg cgggatcggt cgctcgttgg
     4081 gcggcatgcg gcgctgacgc tagcggtcgc ccgcgccgcg cagctgctcg gcggcccgct
     4141 caggggcgac gacgttcagg ttgaggcccg tcccgagctc gaggccggcg aggttggtga
     4201 gccgcgggac gaggtcgatc cgccagcaga agcggtcggc gccgcgcggt gcggtgcgca
     4261 cccagaggtt gaacggcggc accgcgccga gcacgccggc caggcggccg agcacgtcgt
     4321 gcagaagccg cgcgccgagc gggccgtcgt cctcgaagcg cgctcgcggg gtgcgcggga
     4381 cgagctgcac gtggtacggc gtgcgcgagg cgaacgggca gatcgcgacg gcctcgcggt
     4441 cgatcacgac gatgcgctca cggcggcgga cctcctcctg gagcagatcc tcgagcaggt
     4501 tgcggccctg cgtgcggtcc gagtgtgcgg tgaagcgctc gcgctcgcgg gccacggccg
     4561 ccggcacgaa cgcgagcccg tagagctgcg cgtgcgagtg cgggagcgaa gcgccggccg
     4621 cgcggccctc gttgacgatc aggtgaaggc acgcggcgtc cgagttcgca cgcatccgct
     4681 cacgccaggc ctccatcgcg atttcgagct cctccggttc gaggtcgtgc agcgagccga
     4741 ccgcgcgcgg cgtgttcacg atcacctcgt gcgcgccggt cgccgcgcgg gcggcgaaga
     4801 gctgcggctc gccgcggcct gccgccagcg gatcggcagg cgcgtcgccg tcaccgccgg
     4861 acgggcccgc gaggctcggg tagaggttcg gcaccacgcg cacccgccag cccggcgagt
     4921 cgggcggccc gccgcccggg cgcagcgcga acagctcggg cggggtgcgg tcctcgtggc
     4981 cctcgaggaa cgggtcggtc tccgggtcga acggcggctg cggctccacg cgcagctcgc
     5041 cccctggccg ggtggagcgc tcggccgcga cgatcgtgcg cagccccgtc agcgggtcga
     5101 tgcgcaactc aggcaagcgc gcgctccatc gtcacgtggt cgatgccctc ctcgacgaac
     5161 gggtcgccta cctgttcgta gccggcgcgc tcgtagagac cgatggccgc cgactgcgcg
     5221 tgaaggcgca tccgcgtcgc gcccgcgacc cgggccgcgc gctcggcctc cgcgagcacc
     5281 tcccctccga tcccgcgccc gcgcgcaagg ggctcgaccg ccatgcggcc gaggcgagca
     5341 acaccgtcgc gcaccagcac gcggcacgtc cccagcaccc tcccggtgcg caggcacacg
     5401 acgtggatcg cctcgtcgtc gtggccgtcg cgctcggcct cgagggccac gccctgctcg
     5461 ccgcagaaga cgcgctcgcg cagcgcgaga gcctgctcga cgagcccggg gtcgtcggtg
     5521 accgtcgtgg tcatgcgcac gaagctacct ggcgtagcgc tcgatgtcct cgcgcagccg
     5581 ggcctccgtc gcgtagccgc cctggtggat gaaggtgagc ttcccggagc ggtcgtagaa
     5641 ggcggtggtc gggaagttca cgtggccctt gaacacgcgc gcgaccttct ggtccgggtc
     5701 ctcgtagctc gggaagctga cagggaagtc gccgaggaac tcccgcgcgt cctcgcggtt
     5761 gtcctcggag tcgacgccca cgaacgccac ctcgcgaccg agttcgaccg acagccgctg
     5821 gaagtgcggg aactcggctc ggcaaggcgg gcaccacgag gcccacttgt tgatcaccac
     5881 cgggtgaccg cgcagctccc gcagccgccg ctcgaacgcg ggcgcaccgc cgggcaggag
     5941 ccggttcgct tcgcggtgca gcgacgcgag cggcgcgggg gcgccggcga gctggcgctg
     6001 ggcctccgcg cgcgaaggcg cggcactctc gggatcgtcg ttgcagcccg ccaggagggc
     6061 ggccgcgagc agtaacggga cgagtcgccg cacacgctca ggtttgcaca gccgcacctg
     6121 tagtcccgac cgacggcgtg cgttaagatt gcggtgcatt cgcccctcga aagggggacg
     6181 ggcgccgctc cggcgagcca cgctcgaagg cggcggcaca ggggacgtcc ggccgtgtct
     6241 gttctgcggc ctgctcgcat cgcagacccc cgcgcgtccc gaaccacctc ttcatcagag
     6301 agccctcagt tggccgatag caagacgctt actgccgcct cgccggttgc cgaggaagac
     6361 ctcgcacggg tcgaggactt cacgcgcgag ccgttcgtcc acgaggacga ggacccgaag
     6421 gtcgtgctcg ccgcgggcca gccgcgccgg cgcgcgttcg acgcggcggt cgccgagatc
     6481 gaggccgggc acgagaaccc gtcgatcgcg tggcagcagg agttcgcgct gatgctcggg
     6541 ctcgagcggc tcatgtcgca ggatgagccg cacctggccg acggcacgct gctgtccgcc
     6601 caccaggtcg acgcgctctc cggcacgctg atcgcactgc tcgccgaggt gcagagctcc
     6661 gggcgccgca acggcggcgg caacggggcg gtggccgccg aggcgctgag cgagctgccg
     6721 tccggcgagg tcgagctcga gggcgacgag gacatcggcg acgaggacga accgctcgac
     6781 tgggaccccg acgccgaggc cgaggacgag atcgaggacg atgccccgcc gtccgacgac
     6841 ccgggcgccg cgcgtcgctt ctggttcgag cacgcgacgg gcgccggcaa gacggtcgcc
     6901 gcgctggggt tcgtggaggc cacgcgcacg ggcggcgtcc tgatcctcac gcaccggcgc
     6961 aacctcgtcg accagttcct cggcgagctg aaggaccgcg gctacaagaa gctgatcagc
     7021 ccgcctctgc tcgacggcaa gggcgaggac aaggccaagg cgccggtcac ggtcgagacc
     7081 taccagtggt acgtgcgcaa cgccgggcgc atctcgggcg cctactccgt cgtgatctgc
     7141 gacgaggcgc acaccgcgct cggcgagaag accagcgcgt cgatccgctc gtggcagggc
     7201 ccgatcttcg tgggcatgac cgcgacgggc gccctgatcg cccgtcacgt aaccgacctc
     7261 ttccccaccc agacctcgcg cttcgacctc gcgcaggccg cgcgccgggg ggtgatcgcg
     7321 ccgctgcgct gcgtgaggat cccgccgggc gtgggcgtgc gcacgatcgc caaggtgccg
     7381 ctgcgcaagg gcgaggtcga ccaggacttc gaccaggagg agctggccga gctcctcgac
     7441 caggcgccgt tcaacagcgc gatcgccgac ctctaccgcg tccggttcaa ggacctcccc
     7501 ggcgtcgtct actccgccgg cgtgcgccac gcgcacaacg tggcgaaggc gttcgtcgac
     7561 gccgggctca aggccaaggc ggtctcgggc gagacgccga agcgcgagct cacgcgcgtg
     7621 ctcgccgcct acgagcgcgg cgagatcgac gtgctggtga acgcgcagct cctcgccgag
     7681 ggttggaact ctccgcgtgc gaccgtgtgc atgcacctcg cgcctacggc ctcgaagcgc
     7741 atctaccagc aacgcgtcgg gcgcgtcacg cgccgcaacc ccggcaagga ggccggcatc
     7801 gtcgtggact tcgtccaccc ggccaccacg cacgacgatc cggtcgtgac gctgcactcg
     7861 ctgctcgacc gcgacgtcta ccgcggcggc gcgatcgtgg tggggccggt gcggcgcgga
     7921 cgcgggcggc ggctgcgggt cgagcggcgc gtgctgcccg tgaccccgga cccggaccgg
     7981 cgcgccgcgg tgttcgagcg cgagctgtgg cggatcgccg tggagcacct gtcgtggcag
     8041 gagcagcacg tgtgggcggc gctggccggc gcgcgcgtgg cctcgaacaa ctggcgccgg
     8101 gcgaaggcga tgctgcactt cgactcgacc ggcgagctgc gccggcgctt cgttatcacc
     8161 tgcctgcagc gcaaccgcaa cacgcagctg cggatccgcg cgctccagga gatcgcggcc
     8221 gcacgcgacg ccgaggcgtt cgacgaggcc gtggagatca tcggcggctg gtcgcgcgac
     8281 gagcgccgcg agggcgtgaa ggtgctcctc caggcgctgg ccgagcggcc gatcgggcgc
     8341 cgcgaccagg cgaccgcgtg gctgtggcgg ctcgcggaat acacgcgcga ggtgcacgag
     8401 gagtacgccg tgcagcgctg gccggagacc aagcgcctgc tcggcctgct ggtgaactcg
     8461 tcgggcggcg cgcacgcgcg caacgcacgg cggctggtgc aggcggcgcg caagcacgac
     8521 cggcggctct ccgccgccct gctcgccgcg gcgctcgccc acacgcccga ggccgaggag
     8581 gtgctgcgcg gcgcacgcac gcggatggcg cgcaagccga gcgcgctggc gcgcgagctc
     8641 ctgcggaact tcccgaaggg acggcggcgg cgcggcggcc ggcggcgcaa gcgctccggt
     8701 ggcggggagg agaaggccgg cagcgcggac gaggccgccg tgcagaacgc gcagaacggc
     8761 ggcggtgagg aagcggagga gaccgaagcg gacgaggtcg agccccaggc cgacgattca
     8821 tcctcggacg cagcgtcatc cgacgacgac gacgagccgg ccgaggccgc ctgaccgact
     8881 tctgaacact tagtggggct atagccccat caactgttca gacgaaggtg cacctcaccg
     8941 gcgccgagcg ccacgcggcc ctcggaggtg tcgacgagca gggagccgcg gtcgtcgatc
     9001 cccgcggcaa ctccctcccc ctctccccag ccgatgttcg acccgagcag cgcatcgcgc
     9061 gcccgccagg catcgagcac ctcggcagag ggagaagaga gccaccgctc gagcatcgcg
     9121 agcacggcgc tggcggtgtc gtccacggac gcggacgacc cggacaaagc gagcgacgtc
     9181 gccacgaggt ccgcagggaa ctcccgcgtc gtcacgttca ccccgagccc aacgaccgcc
     9241 cagcccgccg ccggccttcc ctccaccagg atcccgcaga gcttgcgtcg cgcgacccac
     9301 acgtcgttcg gccacttgat ctcgcagcgc acgggcgcca gcgactcggc cgcctcgcac
     9361 acggccaccg cggtcgcgag cggcagcagc gcggcggact cgtcgaggtc gcgcagcacc
     9421 acggagagca gcacggcgga gcgcggcgga gcgacccacg agcggccctg acgcccgcgg
     9481 ccggcggtct gctcgtcggc cgtgacggtg gtgccgtgcg gcgcgcccgc gttcgccagc
     9541 tccttggcgc gctggttggt cgagtcggtc acgcggtggt gcacccgcgg cgtgccgatc
     9601 acggtgccgg tgtgatccgc agctcgtcgt cgggctggag ccgcagctcc atcgccgcgt
     9661 tgccacggtt gaccgcgagg gccaccgcgc cccaggcgtc ctcgtagatc aggaactcgc
     9721 ccgccggtac gtcggcgaag gtctgcacga ccgtggcctc acggcggccg ccgcgggcgt
     9781 ggacgagcgc gcggctgccg atgaccagat cgagctcgag caggtgctcg tggcgcaggt
     9841 cgagcgccac gttgccgaag ccgtccacgg cgagtgcgtg ggcgaccacg gcgccgccct
     9901 ctagccgggc gctggggagg tcgagcgcga cgaggtcgcc cgggtcgagc ggatcgcccg
     9961 cgtctgccag ctcagccccg gcggcgaggt gggcggccac gggcgcgaac acgtcgcggc
    10021 cgtggaagct cgccgacacg ggctcgagac ggtggggcga acgactcacg tccaccgcct
    10081 cgaccacgcc gcccgatcgc tgccacgcga ggctgagcac accgttgtcg ggccccacca
    10141 gcagccggtc gtcggcgcag cgcagcgcga gggcgcggcg ctcggtgccg acctgcgggt
    10201 ccacgatcgc cacgtgaacg ccgaccggca tgtacggcag cgccgccgcg agcacgagcg
    10261 cgccgtgcct gaccgcgtga cgctcgatgc cgtgcgcgat gtccacgatc ggcacgtcgg
    10321 gagcgatccg gcggatcacc ccgtgacaga cgccgacgaa ctcgtcgttg cgcccgtagt
    10381 cgctgagcag cgttacgacg atcgggcgtc ctccagtagg gcgtcgacca ggccgtccac
    10441 gtcgtggcgc tcggcctcca cgtgcacctc gagcccgagc tcgcgtgcgg tggcgctcgt
    10501 ggcggggccg atcgagatca cgcgcgcgcc gcgcgggaac gcgtcgccgg cggcctcgag
    10561 gaagtagcgc acggtggagc tggaggtgaa cgtgacgaag tcggcgcggg cggcggcctc
    10621 gagcgtcggg gccgcgggcg agtcggcaac cgtgcggtac agcgcgacca cgtccaccgc
    10681 ggcgccgcgc tcgcgcagcg cgtcgggcac cacatcgcgg ccctcctcgg cgcgggcgaa
    10741 caggacgcgc ctgccgtcca cctcctcgcc ggccaaagcg gcgaccaggc cctcggccat
    10801 ctgccgctcc ggcaccacgt ccgggtcgat cccgcgggcg gcgagggccg ccgcggtggc
    10861 ggtgccgatg gtcgccacgc gcgcgcccgc cagcgcgcgg gcgtccgtgc cctgccgcct
    10921 gagcgcgtcg aagaacggct cgatgacatt ctggctcgtc aggcagatca ggtcgtagcc
    10981 gtcgatcggc ggcagctcga cgctgggcag cggctcggtg cggatcaccg gcgcctcgac
    11041 cacctccgcc ccgaggcccg cgagccgagc cgcgagcgcg ctcgcctggt cccgcgcgcg
    11101 cgtgaccacc acgctcctgc cgagcagcgg gcgaccggcc tcgaaccacg cgagccgctc
    11161 gcgcaggtcc gccaccgcgc ccaccacggt gagcgcgggc ggcttcacgc cggactcggc
    11221 cacgcgggcg gcgatgtcgc cgagggtgcc ggtcacgacg cgctggcgcg cggtggtgcc
    11281 gcgctcgacc acggctgccg gttcgccctc cgagcgcccg gcctccacga gcgaccacgc
    11341 gatccgctcg aggttgcgca cgcccatgta gaagacgagc gtgccgggga acgcggccag
    11401 cgcgtcccag tcgatcaccg actccggctt ggtcggatcc tcgtggccgg tgacgaacgc
    11461 gaccgcggac gctccgtcgc ggtgggtcac ggggatcccc gcgtacgcgg gcgccgcgac
    11521 gcccgcggtg acacccggca cgacctcgaa ctcgaggccg gcgcctgcca gcgcctcggc
    11581 ctcctcgccc ccgcgaccga acacgaatgg gtccccgccc ttgagccgcg ccaccatccg
    11641 gccggcacgg gtgtgctcga ccaggcgcgc gttgatctcg tcctggggca tcgcggctcc
    11701 gcccggctcc ttgccgacgt agaccagctc ggcccggagg ccgtcgagca cggcgggggg
    11761 cacgagccgg tcgtagagca ccacgtcggc ggcggcgagc agctcgcgcg cgcgaaccgt
    11821 gatcaggccc gggtcgccgg gaccggctcc gatgaggtac gccttgccgg ccacgcggca
    11881 accctagccc cgatcatttg tccagagatc gcccgtctca ttacatccgg gttacggtgg
    11941 aggtccggtc acggatgacc ccctcacacc tcctgaaggg actcgcatga agcgcatcgc
    12001 cggcctcgcg gccgctctcg ccgtcgcctc gctcgtatcc gccgcgcccg tcctggccgc
    12061 gccggccacc atcggctcga ccggcatgac ctacgcgaac ggcgggatca gctgcatctc
    12121 cgcgcttcag ggggcgaccg cgccgggctc gcccgcctac gtcgtgccgt tcgacggcac
    12181 gatcgcgagc tggagcgtgc agggcgcgaa ccagccggcc accgctcggc tccaggtcta
    12241 ccgcccgctc ggtccgaacg agtggcggct ggtggccgag agcgccgtgc gccaggtgtc
    12301 cgcgaccggc gtgaccacct ccgccgcgcg gatcgccgtg cgccagggcg acatcatcgg
    12361 tcgaaccggg ctcggctgcg tctacacgag cgcgctgtcg gcggacgatg tccgcacgtt
    12421 cggttatcac ccgaccgtcg ggtcggtcgc gacaccggac gggccgggcg cgagcgcctg
    12481 gcgcctgaac cttcgagcca ccgtggagcc agactgcagc aaggggaacc gcaagaagcc
    12541 gaaggtctgc agggccgccc gccgctgagg cggtcgccgg cgctgccgct cgcggcgggc
    12601 gtcgcggcgc tgctcgtcct gttcgccggc cgctacgggt accaccgcga cgagctgtac
    12661 ttcgtggagg ccggccggca cctggcgtgg ggctacgtcg accaagggcc gctaacgccg
    12721 ctcctggcgc gcgcgatgac cgagctgtcc gagggctcgc tcacggtcct gagggtcccg
    12781 tctgcgctca tgtgcgcggg gatggtgctg ctcacgggca tgctcgctcg ggagctcggg
    12841 ggaacccgcc gcgccgagct gatcgccgcc ggctgcacgg cggtgggggt cgtgttcctg
    12901 acgaccggcc acctgctcag cacaaccacg ttcgacctgc tcgtctggat cgcggtgacc
    12961 tacctcgcgg tgcgcgccgt gcgccgcggc gaggagcggc tgtggccggt ggccggcgtg
    13021 gtgctcggcc tcggtctgct caacaagccg ctgccagcgt tcctggccgc cgggctccta
    13081 gcgggcgtcg ccatcgccgg gccgaggcgg ctgatccgca gtccgtgggt ctgggcaggt
    13141 gcggcgatcg cgctcgtgat gtggatgccc tggctcgtgt ggcaggcgga ccatggctgg
    13201 ccgcaggcgg aggtctcgcg cgcaatcgcg gagggcggct cgacctcgtc ggagccgcgc
    13261 tgggcgatcg tgccgttcca gttcctgctg gtcagcccgc ccctcgcacc ggtgtggatc
    13321 gccgggctcg tacggctgtt ccgcgacccg gtcctgcgcg acctgcgctt cttcgcgtgg
    13381 acgtgggtcg cgctcgcgat cgcgttcatg gcgaccgggg gcaagccgta ctacctcgcc
    13441 gggctgctgc cgctgctgct cgccgccggc gcgggcgcgg tggacggctg gctcgagcgt
    13501 gggcggcgcg gggcccggcg ggtcgcgctc ggcgcggcgt tcgcgctgag cgtcgcgatc
    13561 ggcgtgccga tcgcgctgcc ggtgctcccg gtcgaggaca tcgagccggt gctcgcggcc
    13621 aacgacgacg tgggcaacac cgtcggctgg cccggtttcg tcgatcaggt cgcggcggtt
    13681 tacaggcgcg tgccgggcgc ggtgatcctc acgagcaact acgggcaggc gggcgcgatc
    13741 gaccgctacg gcccggacct ccgcctcccg cgcgcgtaca gcggccacaa cgcgtaccac
    13801 gagtggggcc cgccgcccga ccgcggcggg ctggtgatcg tgatcgggga ggtcgagcgc
    13861 gaggggttcc gcggttgcac gctcgcgcac cggatcgaga ccgtggcgga cctggacacc
    13921 gaggagcggg gcaccgaagt ctggacgtgc cgggggacgg ccaggccgtg gtccgagctg
    13981 tggcccgagc tcgagcgctt cggctgatca ggcggcggcg cgctcggcgg cggccagc
//
LOCUS       DASVPL010000271         4866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_306310, whole genome shotgun sequence.
ACCESSION   DASVPL010000271 DASVPL010000000
VERSION     DASVPL010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306310"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF517_15065"
     CDS             complement(<1..402)
                     /locus_tag="VF517_15065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054307.1"
                     /translation="MLGDELIELLGDAIPADHHRQTLADDLLIARYGPQAGDRVLDLG
                     CGAGDSVEQFGSLNPNVRWVGVDLEESPEVALRTRTDAEFRTFDGRSIPAGDASVDVV
                     YCKQVLEHVEERAPLIADVARVLRPGGLFLGS"
     gene            complement(396..1109)
                     /locus_tag="VF517_15070"
     CDS             complement(396..1109)
                     /locus_tag="VF517_15070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054308.1"
                     /translation="MKDLYTPDNLLDRLSDPATLQVHYHHLQDREREVLARRLPLGGG
                     DVLSVGCGPNPGRHLFPAPRFRLTGADVDPDYVEQARGQADETVLARAGELPFEDGSF
                     DVVLYRLVLHHVAYQGPLRPVFEEAARLLKPGGAAVAIEPGLWHPVGAALALANRSGA
                     VATRIHGSPDDIPLSPRALTRDARAAGLQPELHALTYTWRRMPPPAQRALQPLDRIGS
                     RPRAALFGHHLLLIARRPC"
     gene            complement(1106..1270)
                     /locus_tag="VF517_15075"
     CDS             complement(1106..1270)
                     /locus_tag="VF517_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054309.1"
                     /translation="MAWDDDEDWVGEPPEGRHSRDRAKPEFWRQNWPVSVTGAGMVIA
                     LIILLIVLLT"
     gene            1301..3616
                     /locus_tag="VF517_15080"
     CDS             1301..3616
                     /locus_tag="VF517_15080"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX8054310.1"
                     /translation="MTLLYSLALLVSAALLFLLEPMVGKFMLPSLGSTPEVWTTTVLF
                     FQVVLLAGYGYAHVTSRLAPRRQALLQLGLLAVAALVLPIGVPDARPPDDGNPVPWLL
                     GVLAVTAGPPFLVLAANGPMLQRWLAGTRHRAGRDPYFLFAASNGGSLIGLLAYPLVV
                     ERVLTLDGQGELWAAAYGAAALLVLASAVVLWRAEPAAEPAEAAPPDAGGSPPAAASP
                     AAAAAPPATAEAAPDAGGLPPAAASPAAAATPSWRRRLTWLALAAVPSSLMLGTTTYL
                     TRDLSPVPLLWVVPLALYLLTLVIAFAPGADAARLTVWGRRLLPGIAILVAYTLAIGA
                     QQPLFGLVALHLIGLVVAGLLCHARLAADRPGAERLTEYYLWVALGGALGGAFNALFA
                     PVAFPGLVEYPLALVAACMLRPAPPKKRPDVLEFFLRDERPTRYMDFAAPVLIGAAIA
                     GILLLAREDDGSVPLEVRGAVIGVALGMIVNSARRPIRFALTLGAIILAAQTGATPGQ
                     DLLERDRSFFGIYRVVESEGGRVHDLYDGSTLHGSQRVNDRDPLTYYSRVGPAGQALA
                     GLPRAATRRVAAVGLGAGALACHARPGDEYTYFEIDPVVARIARDPDYFTYLRDCGNP
                     RVVLGDGRRSLARHRGAPFGLIALDAFNSDAIPVHLVTREAIELYLRRLAPRGALMVH
                     LSNRYLDLEPVMGNLARDLGLACRIERHEPTDAQHERGYTASVWALLVRDPSHLGPLA
                     RDPRWHACEDDPGAETWTDDYSNLLGVVDWS"
     gene            3737..>4866
                     /locus_tag="VF517_15085"
     CDS             3737..>4866
                     /locus_tag="VF517_15085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8054311.1"
                     /translation="MPPTETTTDPPNAPRDLRPRPSREEIEPAALALIRRHGGEVLAV
                     ARRWADSPEDAEDAYQRGLEIMLTKAPSTDPEHLVPWLKTVVKREAWAIRRQRERHTP
                     SAPESEELDGTAPGSAHDHAVRLERLRLGAEAMRRLKPQEVRALTLKAEGLSYREICD
                     ETGWSYTKVNRCLSEGRRRFHDRLAGIESGAECERLAPHLSALADGEARAEDMAALRP
                     HLRTCLVCRARLRDYRAVPARVAAVSPPVATAGSLLGPARELLHGSSGWISERSAALA
                     ARWHQATELALAHKGVAVVASAAVLGGGGAATVATVDGFEPGRHAATASAPERTTLPP
                     LPPATASLAARAADDHDRHASAKRDPQPPAKRDRRAAAATPVT"
BASE COUNT          577 a         1841 c         1813 g          635 t
ORIGIN      
        1 cgagccgagg aacagcccgc ccggccgcag cacgcgtgcc acgtcggcga tcaggggcgc
       61 gcgctcctcg acgtgctcga gcacctgctt gcagtagacg acatccacgg aggcgtcgcc
      121 ggcggggatc gagcgcccgt cgaaggtgcg gaactcggcg tcggttcgcg tgcgcagggc
      181 cacctcaggc gactcctcga ggtccactcc gacccatctg acgttcgggt tgagcgagcc
      241 gaactgctcg accgagtcgc ccgcgccgca gcccaggtcg agcacacggt cgccggcctg
      301 cgggccgtag cgcgcgatca ggaggtcgtc ggcgagcgtc tggcggtggt ggtcggcggg
      361 gatcgcgtcg cctagcagct cgatcagctc gtcgcctagc acggccggcg cgcgatcagg
      421 aggaggtggt ggccgaagag cgcggcgcgc gggcgcgatc cgatccggtc gagcggctgg
      481 agcgcgcgct gggccggcgg cggcatgcgg cgccaggtgt aggtgagggc gtggagctcg
      541 ggctggaggc cggcggcgcg agcgtcgcgt gtgagagcgc gcggcgagag cgggatgtcg
      601 tcgggcgagc cgtggatgcg ggtggcgacg gcgccgctgc ggttggcgag cgcgagcgcc
      661 gcgccgactg ggtgccacag gcccggctcg atcgcgaccg ccgcgccgcc gggcttgagg
      721 aggcgggccg cctcctcgaa gacgggcctc agggggccct ggtaggcgac gtggtggagc
      781 acgaggcggt agagcaccac gtcgaaggag ccgtcctcga agggcagctc gcccgcgcgc
      841 gcgaggacgg tctcgtcggc ctggccgcgc gcctgctcga cgtagtcggg gtcgacgtcg
      901 gcgccggtga gccggaagcg cggcgcgggg aacaggtggc ggccggggtt gggaccgcag
      961 ccgacgctca gcacgtcgcc cccgccgagc ggcagccgcc gggcgaggac ctcgcgctcg
     1021 cggtcctgga ggtggtggta gtggacctgg agggtggcgg ggtcgctgag ccggtcgagc
     1081 aggttgtccg gcgtgtagag gtccttcaag tcagcaggac gatcaggagg atgatcagcg
     1141 cgatcaccat gcccgcgccg gtcacgctca ccggccagtt ctgacgccag aactccggct
     1201 ttgcgcggtc gcgcgagtgc cggccctccg gcggctcgcc gacccagtct tcgtcgtcgt
     1261 cccaggccat tggtcgctcc gggtgcgaca gtagccgacc gtgacgctcc tgtactcgct
     1321 cgcactgctc gtcagcgcgg cgctcctgtt cctgctcgag ccgatggtcg gcaagttcat
     1381 gctgccatcg ctcggctcca cgccggaggt gtggaccacc accgtcctgt tcttccaggt
     1441 cgtgctgctc gcgggctacg gctacgcgca cgtgacgagc cggctcgcgc cgcggcgcca
     1501 ggcgctgctc cagctcgggc tgctcgcggt ggctgcgctg gtgctgccga tcggcgtgcc
     1561 ggacgcgcgc ccgccggacg acgggaaccc ggtgccgtgg ctgctcgggg tgctggcggt
     1621 cacggccggt ccgccgttcc tggtgctcgc ggcgaacggg ccgatgctcc agcgctggct
     1681 cgccggcacc cggcaccgcg cagggcgcga cccgtacttc ctgttcgcgg cgagcaacgg
     1741 cgggagcctg atcgggctgc tcgcgtaccc gctcgtggtg gagcgggtgc tgacgctcga
     1801 cgggcagggc gagctctggg cggcggcgta cggcgcggcg gcgctgctgg tgctggcgag
     1861 cgcggtggtg ctgtggcggg cggagccggc ggctgagccg gcggaggcgg ccccgccgga
     1921 tgccggcggc tcgccacccg ccgccgcttc gccagccgcc gccgctgcgc cacccgccac
     1981 cgcggaggcc gcgccggatg ccggcggctt gccccccgcc gccgcttcgc cagccgccgc
     2041 cgcgacccct agctggcgcc gccgtctcac gtggctcgcc ctcgccgccg tcccgtcgag
     2101 cctcatgctc ggcacgacca cctacctcac gcgcgacctg agcccggtcc cgctgctctg
     2161 ggtggtgccg ctcgcgctct acctcctcac cctggtgatc gcgttcgcgc ccggcgccga
     2221 cgcggcgcgg ctcaccgtct ggggccgcag gctcctcccc ggcatcgcga tcctggtcgc
     2281 ctacacgctc gcgatcggcg cccagcagcc gctgttcgga ctcgtggctc tccacctgat
     2341 cgggctcgtc gtcgccgggc tgctctgcca cgcccggctc gccgccgacc ggcccggtgc
     2401 cgagcgcctg accgagtact acctgtgggt cgcgctcggc ggcgcgctcg gcggggcgtt
     2461 caacgcgctg ttcgcgccgg tggcgttccc ggggctcgtc gagtacccgc tcgcgctcgt
     2521 ggcggcgtgc atgctgcgcc ccgcgccgcc caagaagcgg ccggacgtgc tcgaattctt
     2581 cctccgcgac gagcgcccca cgcggtacat ggacttcgcg gcgcccgtcc tgatcggcgc
     2641 cgcgatcgcc ggcatcctgc tcctcgcgcg cgaggacgac ggcagcgtgc cgctcgaggt
     2701 gcgcggggcg gtcatcgggg tggcgctcgg gatgatcgtc aacagcgcgc ggcgcccgat
     2761 ccgcttcgcg ctcaccctcg gcgcgatcat cctcgccgcg cagaccggcg cgaccccggg
     2821 tcaggacctg ctcgagcgcg accgcagctt ctttgggatc taccgggtgg tcgagagcga
     2881 gggcgggcgc gtgcacgacc tctacgacgg ctcgaccctc cacggctcgc agcgcgtgaa
     2941 cgaccgcgac ccgctcacct actactcgcg cgtggggccg gctggccagg cgctggcggg
     3001 gcttccccgc gccgccacgc ggcgagtcgc cgcggttggc ctcggcgccg gcgcgctggc
     3061 ctgccatgcg cggcccggcg acgagtacac gtacttcgag atcgatcccg tcgtggcccg
     3121 gatcgcccgc gaccccgatt acttcaccta cctgcgcgac tgcggcaacc cgcgcgtggt
     3181 gctcggcgac ggccgccgct cgctggcccg ccaccgcggc gcgccgttcg gcctgatcgc
     3241 gctggacgcc ttcaactcgg acgcgatccc ggtccacctc gtcacgcgcg aggcgatcga
     3301 gctgtatctg cggcggctcg caccgcgcgg cgccctgatg gtccacctct cgaaccgcta
     3361 cctcgatctc gagccggtga tgggcaacct cgcgcgcgac ctcggattgg cctgccgcat
     3421 cgagcgccac gagccgaccg acgcgcagca cgagcgcggc tacaccgcgt cggtgtgggc
     3481 gctgctcgtg cgcgacccct cgcacctcgg gccgctcgcg cgcgaccccc gctggcacgc
     3541 ctgcgaggac gaccccggcg ccgagacctg gaccgacgac tactcgaacc tgctcggcgt
     3601 ggtggactgg agctgatcgg gaggccctga tcggcccccc atccgggggc agtcatccgg
     3661 ccgcggggcg gaatttccgg ggtgaggggg gcgagtccct tgcgccgcgc gctgtacgcg
     3721 gcagaggatc gccacgatgc ccccaaccga gacaaccaca gacccgccga acgccccgcg
     3781 tgacctgcgc ccgcggccgt cgcgcgagga gatcgagccc gccgcgctcg cgctgatccg
     3841 ccgccacggc ggcgaggtgc tggcggtcgc acgccgctgg gcggactcgc cggaggacgc
     3901 cgaggacgcc taccagcgcg ggctcgagat catgctgacc aaggctccgt cgaccgaccc
     3961 cgagcacctc gtcccgtggc tgaagaccgt ggtcaagcgc gaggcgtggg cgatccgccg
     4021 ccagcgcgag cggcacacgc cgagcgcacc cgagtcggag gagctcgacg ggacggcgcc
     4081 cggcagcgcc cacgaccacg ccgtgcgtct cgagcggctc cggctcggcg ccgaggcgat
     4141 gcggcggctc aagccgcagg aggtccgcgc gctcacgctg aaggccgagg gactctcata
     4201 ccgggaaatc tgcgacgaga ccggctggag ctatacgaag gtcaacaggt gcctatcgga
     4261 gggccggcgc cgcttccacg accgcctcgc gggcatcgag agcggcgccg agtgcgagcg
     4321 gctcgctccg cacctctccg cgctggccga cggcgaggcg cgcgccgagg acatggccgc
     4381 ccttcgaccg cacctccgca cctgcctggt gtgccgggca cggctgcgcg actaccgcgc
     4441 ggtgccggcc cgggtggcgg cggtctcccc gccggtcgcc accgccggct cgctgctcgg
     4501 cccggcccgc gagctactcc atggctcgtc aggctggatc tccgagcgca gcgccgccct
     4561 cgcggcgcga tggcaccagg cgaccgagct cgcgctggcc cacaagggcg tcgccgtggt
     4621 cgcgtcggcc gccgtgctcg gcggcggcgg ggcggccacg gtcgccacgg tcgacggatt
     4681 cgagcccggg cgccacgcgg cgacggccag tgcgccggaa cggacgaccc tgcccccgct
     4741 gccgcccgcg accgcgagcc tcgccgctcg cgccgccgac gaccacgacc gccacgcgtc
     4801 cgccaagcgc gacccacagc cgcccgcgaa gcgcgaccgg cgcgccgcgg cggcgacgcc
     4861 ggtgac
//
LOCUS       DASVPL010000272         9988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_228378, whole genome shotgun sequence.
ACCESSION   DASVPL010000272 DASVPL010000000
VERSION     DASVPL010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228378"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /gene="ftsH"
                     /locus_tag="VF517_15090"
     CDS             complement(<1..1451)
                     /gene="ftsH"
                     /locus_tag="VF517_15090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX8054312.1"
                     /translation="MSRFFKSAAFPILIVVILAFFAQKLISPGEEKKAPNYGQFVTQV
                     NAGQVKKATLNTKDNTIDFETTATEGGKRQKFTTAYPDNTEQGLVNTLLSKNVEVDVK
                     GKGGGGWLSVLTYVLPFVIFLIFWLFIINQMQGGGSKVMSFGKSRAKRMSVDSPKITF
                     RDVAGVDEAVEELHEIKEFLENPKKFQALGARIPKGVLLYGPPGTGKTLLARAVAGEA
                     GVPFFSISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFMDEIDAVGRHRGAGLGGG
                     HDEREQTLNQLLVEMDGFEMKDNIILIAATNRPDILDPALLRPGRFDRQIVVDRPDRK
                     GRARILEVHTRGKPLAREIDLDNLAAQTPGFTGADLSNLVNEAALLAARQGKREIDHT
                     HLEEGIMRVIAGPEKKTRVMSEKERKVTAFHEMGHAIVAHFLEHTDPVHKISVISRGQ
                     ALGYTISLPTEDKFLTTRAELSDTMAMTLGGRAA"
     gene            complement(1574..2110)
                     /gene="hpt"
                     /locus_tag="VF517_15095"
     CDS             complement(1574..2110)
                     /gene="hpt"
                     /locus_tag="VF517_15095"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX8054313.1"
                     /translation="MPATDTLIGETLVAEGPLAERVHELGREISADYAGRDLVLVGVL
                     KGAVFFLSDLMRHLDVRCEVDFMAVASYGSSTDSSGVVRILKDLDTSIEGREVLIVED
                     IVDSGLTLSYLLRTMRARNPASLEVCALLTKPERRKVELPIRYTGFEIPNRFVIGYGL
                     DYAEKYRNLPFVAVLNNP"
     gene            complement(2121..3386)
                     /gene="tilS"
                     /locus_tag="VF517_15100"
     CDS             complement(2121..3386)
                     /gene="tilS"
                     /locus_tag="VF517_15100"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX8054314.1"
                     /translation="MERALDAARRSGLIRAGEPLLVMLSGGGDSVCLLDVTVRLGAEV
                     SALHVDYGLRADSGADAELCRELCERLGVGLTVERVELGGAGNLQAEARDARYALAER
                     HARGDYAAAHTASDQAETVLYRLAVSPGRRALLGMQPRRGRLVRPLLAITREEAREHC
                     RAHGLEWREDPSNSDLRFARARVRTEVLPALRELNPAAERNIAETAAQLREEADVLDV
                     ALREALGRPAASPLPLEALRALPAPLARLALRAAAGDVAVSRADAQRILELPLEGTAT
                     VELPGGVRAISEYGSLRFATGGEPEPPPRAVALPIPGAVDFGRWRVEAARGGAGDVLL
                     DPAAASAGLVVRPWRHGDRMRPAGLGGTKTLQDLFTDRKVPRAERGSVPVVEAGGSVA
                     WVAGVAVDERFRADPADAARAVALTARHA"
     gene            complement(3388..4581)
                     /locus_tag="VF517_15105"
     CDS             complement(3388..4581)
                     /locus_tag="VF517_15105"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8054315.1"
                     /translation="MSERLLSLREAADSVGVTQATLRRWAQTGVIPEHDGSAEWTPAA
                     VAHARMVARLRERGHTLQALRKAGEEGRLAHGYVEALLVPENRGSIPFEEAADAVGLE
                     PSMVERIWNTLGFAPRRPETISDEDLKALRYVASVLDAGFPLVAFLQLLRVYGQSLAR
                     IADAETRLFHIYVHEPLMRQGVPGLQMAEEMEDLAGDLLPLASPLMDYLHQRFLAEFV
                     ERDVVGHMEVELDESIDLGRVRVTVAFADLAGYTRFTEEAGEEEALTTVERFIDAVTE
                     TLPDDARVIKTIGDEVMIVATDPGALTDWSVGFQRLWDERPTPRIGIHAGVALYRDGD
                     YYGRDVNLAARVVARARGGEVVVSDAVMDEIREDEHLEFENIGQVKLKGFDEPRRLCR
                     VRGRE"
     gene            4644..6668
                     /locus_tag="VF517_15110"
     CDS             4644..6668
                     /locus_tag="VF517_15110"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8054316.1"
                     /translation="MSYFVTGATGFIGRHLVELLLKREGTIYALVREGSRGRLDELAQ
                     RLDAGDRIVPVVGDLTKEGLGVEGFDERIDHFFHLAAIYDMAADEETMLKSNIEGTRH
                     VAQFANSIDVGTFHHTSSIAVAGKFRGLFREDMFDEEQKLPHAYHQSKFESERVARTE
                     VKAPLRVYRPGIVVGHSLTGEMDKVDGPYYFFKLIQRIRGALPQWFPIVGIEGGKANL
                     VPVDFVARAMDHIAHVDADDLPCHTFHLVDPNPLTVGQAMNEFARAAHAPQFAMRMDS
                     NITDVVPKPVRAGLNAIPTVKNIRETLLRDLHIPPAALENREFEADFDARETQRALSG
                     TGIAVPPLSTYAAKLWDYWERNLDPDLFRERSLTKSIEGKRILVTGASSGIGRDLAMK
                     LADAGGEVILVSRTREKLEEVADAMRKAGGNPHVHPADLSDLEDVERLANEVIEEYGG
                     IDILVNNAGRSIRRSLKASHERFHDYERTMQLNYFGALRLIMSFIPGMRERRAGHIIN
                     VSSIGVQTNTPRFSAYVASKAALDAFSRCVAPEVIGDGVHITTIYMPLVRTPMIAPTN
                     IYDAFPTLSPDEAAQLITDAMIDKPKRVATRLGTFGQILYSVSPKVVDQVMNTAYNIF
                     PDSKAAKGDKKPSAEASGDGDGARDGGRDEKQEMSTEAIAMAYLLRGVHF"
     gene            6900..7544
                     /locus_tag="VF517_15115"
     CDS             6900..7544
                     /locus_tag="VF517_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054317.1"
                     /translation="MTTPIDITDPRLVKAYAHPLRIEILSLLDNRVASPKQIATELET
                     PLPNTAYHVRQLAALGLVELVRRTVRGGAVEHHYTTKVRPTIPDELWSTLPDIVRRAA
                     MGGAVQQALSEMIRAVDQGGFSPDEAHLTRTPARLDAKGWRAVSREMHRALKRVDAIV
                     AETEARLENDPEEETVEAMAVMALFERPEMERDGAAGAPRRTGRTPAQAGRRFS"
     gene            complement(7541..7966)
                     /locus_tag="VF517_15120"
     CDS             complement(7541..7966)
                     /locus_tag="VF517_15120"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8054318.1"
                     /translation="MSFNSARECREVMDRVFEMMSTDPDMGPKLHDADTPQRFEFPDL
                     DLVVNVAPASDAGEDGAHLRWEWSDDVDWEPEVKMTMDSDVANRYFQGKENVAMAIAR
                     RRIKTSGNVKKALALIPITKPVYEQYRALVERDYPHLAA"
     gene            complement(7976..8914)
                     /locus_tag="VF517_15125"
     CDS             complement(7976..8914)
                     /locus_tag="VF517_15125"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8054319.1"
                     /translation="MSDGPLGPAVSFLRALRGGLEGGAAPATAFAGAAMGLPREARES
                     LEAVMRRLGGEYEEDDFGLDEEFVEAALPFLEFLYDRWWRIQVEGVTNVPSHGRALLV
                     ANHAGILPWDAAMIGVALQREHPLPRYPRFTVLDWAFELPYVSVAIRKLGGVPASPYN
                     ALRLLDQEELVAVFPEGTNGAQKLYRDRYQLRRFGRGGFVEIALRTRAPIVPVAIVGS
                     EEVHPKLADAPLLARLAGTPFFPVTPTFPALGPLGAVPLPSRWRMEFCEPIDLSEFGP
                     DAAADRTLVFELSEHVRDVIQKKVWENLAKRRSAFL"
     gene            complement(8911..9879)
                     /locus_tag="VF517_15130"
     CDS             complement(8911..9879)
                     /locus_tag="VF517_15130"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8054320.1"
                     /translation="MPGRRILITGIASYLGTELARRLEDDPGVEYVVGIDTKRPRAPL
                     RFTEFIDADIRDPEIGRLIPPTGVDTVVHNQIVRQPGPGMSARAAHDINVIGSLQLLA
                     ACERIETLRTIVVRGSAGIYGSEPAAPQFFTEDMSRLYPLRTRFQRDVAEIENLFETF
                     RRRHEDVVCTMLRYQLTIGPAHATQITRYLSLPVVPTYLGFDPRIQLIHERDALDAFV
                     AAVRNPVRGAVNVAARGTLGLTRAIRLVRRPTMPVPAPLFGTTSQLGRRLGLFSFSPD
                     FRRLLRYGRGVDTTRLVEEVGFTPRFSTVEAVRDYTAARDSTAAAA"
BASE COUNT         1526 a         3473 c         3567 g         1422 t
ORIGIN      
        1 gcggcgcggc cgccgagcgt catcgccatc gtgtccgaca gctccgcgcg cgtggtgagg
       61 aacttgtcct cggtcggcag cgagatcgtg tagccgagcg cctggccgcg cgagatcacc
      121 gagatcttgt ggaccgggtc ggtgtgctcg aggaagtggg cgacgatcgc gtggcccatc
      181 tcgtggaagg ccgtgacctt gcgctccttc tcgctcatca cgcgggtctt cttctcgggc
      241 cccgcgatca cgcgcatgat cccctcctcg aggtgcgtgt ggtcgatctc gcgcttgccc
      301 tgacgggcgg cgagcagggc ggcctcgttc acgaggttcg agaggtcggc accggtgaag
      361 cccggtgtct gcgcggcgag gttgtcgagg tcgatctcac gcgcgagcgg cttgccgcgg
      421 gtgtgcacct cgaggatgcg ggcgcggccc ttgcggtcgg ggcggtcgac cacgatctgg
      481 cggtcgaagc ggcccgggcg cagcagcgcg gggtcgagga tgtcgggccg gttggtggcc
      541 gcgatgagga tgatgttgtc cttcatctcg aagccgtcca tctccacgag gagctggttg
      601 agcgtctgct cgcgctcgtc gtggccgcct ccgaggccgg cgccgcgatg gcggccgacg
      661 gcgtcgatct cgtccatgaa gatgatgcag gggctgttct gcttggcctg ctcgaacagg
      721 tcgcgcacgc ggctggcgcc gacgcccacg aacatctcga cgaagtccga gcccgagatc
      781 gagaagaacg gcacgcccgc ctccccggcg acggcgcggg cgagcagcgt cttgccggtg
      841 cccggcgggc cgtagagcag gacgcccttc gggatccgcg cgccgagcgc ctggaacttc
      901 ttgggattct cgaggaactc cttgatctcg tggagctcct ccaccgcctc gtccaccccg
      961 gccacgtcgc ggaaggtgat cttcggcgag tcgaccgaca tccgcttcgc gcgcgacttg
     1021 ccgaaggaca tgaccttcga cccgccgccc tgcatctggt tgatgatgaa cagccagaag
     1081 atcaggaaga tcacgaatgg cagcacgtag gtgagcaccg acagccagcc gccgcccccc
     1141 ttgcccttca cgtcaacctc gacgttcttc gagagcagcg tgttgacgag gccctgctcg
     1201 gtgttgtccg ggtaggccgt cgtgaacttc tgacgcttcc cgccctcggt ggcggtcgtc
     1261 tcgaagtcga tcgtgttgtc cttggtgttg agggtcgcct tcttgacctg acccgcgttc
     1321 acctgcgtga cgaactggcc gtaattcggc gccttcttct cctcacccgg ggagatgagc
     1381 ttctgcgcga agaacgccag gatcaccacg atgaggatcg gaaaggcggc gctcttgaag
     1441 aatcggctca caggcctctg tttcctggtc gggggaagaa cccctactac gcgtaacgac
     1501 gcagcgtagc aagggtagtt ggagggtttt gctgcttttc ggctggttcg gaggccagcc
     1561 taaagcggat cactcaaggg ttgttcaaga ccgccacgaa cggaaggttg cggtacttct
     1621 ccgcgtagtc gagcccgtac ccgatcacga accggttcgg gatctcgaag ccggtgtagc
     1681 gaatcggcag ctcgaccttg cgccgctccg gcttggtcag cagcgcgcag acctcgagcg
     1741 acgccgggtt gcgcgcccgc atcgtcctca gcaggtagga cagcgtgagg cccgagtcga
     1801 cgatgtcctc gacgatgagc acctcgcggc cctcgatgga ggtgtcgagg tccttgagga
     1861 tgcgcacgac gcccgacgag tcggtcgagg agccgtagga ggcgaccgcc atgaagtcga
     1921 cctcgcaccg gacgtcgagg tggcgcatga ggtcggagag gaagaacacc gcgcccttca
     1981 ggactccaac gaggaccagg tcgcggccgg cgtagtcggc ggagatctcg cggccgagct
     2041 cgtgcacccg ctcggcgagc ggcccctccg ccacgagcgt ctcgcctatc agggtgtcgg
     2101 tggcgggcac tgcggcgagt ctatgcgtgg cgcgccgtga gtgccacggc ccgcgcggca
     2161 tccgccggat cggcgcggaa acgttcgtcc acggccaccc ccgcgaccca ggcgacgctg
     2221 ccgcccgcct cgaccaccgg gaccgagccg cgctccgcgc gcgggacctt gcggtcggtg
     2281 aacaggtcct ggagcgtctt cgtgcccccc aggccagccg ggcgcatccg gtcgccgtgg
     2341 cgccacgggc gcaccacgag cccggcggac gccgcggccg ggtcgagcag cacgtcgccg
     2401 gcgccgccgc gggcggcctc gacccgccag cggccgaagt cgacggcgcc ggggatgggc
     2461 agcgcgacgg cccgcggagg aggctccggc tccccgccgg tcgcgaagcg cagcgacccg
     2521 tactcggaga tcgcgcgcac gccgccgggc agctcgacgg tcgcggtgcc ctcgagcggg
     2581 agctcgagga tgcgctgggc gtccgccctc gagaccgcga cgtcgcccgc ggccgcgcgc
     2641 agcgcgagcc gcgcgagggg cgccggtagc gcgcgaaggg cctcaagcgg cagcgggctc
     2701 gcggccgggc ggccgagggc ctcgcggagc gcgacgtcga gcacgtccgc ctcctcgcgg
     2761 agctgcgcgg cggtctcggc gatgttgcgc tcggccgcgg ggttcagctc gcggagcgcc
     2821 ggcagcacct cggtcctcac gcgcgcgcgc gcgaaacgaa ggtccgagtt cgacgggtcc
     2881 tcgcgccact cgagcccgtg cgcgcggcag tgctcgcgcg cctcctcgcg cgtgatcgcg
     2941 agcagcggcc gcaccagcct gccgcgccgc ggctgcatcc ccagcagcgc ccgccggccg
     3001 ggcgataccg cgagccggta gagcacggtc tcggcctggt cggaggccgt gtgcgcggcc
     3061 gcgtagtccc cgcgtgcgtg gcgctcggcc agcgcgtagc gggcatcgcg cgcctcggcc
     3121 tggaggttgc ccgcgccacc gagctcgacc cgctccacgg tcaggcccac gccgagccgc
     3181 tcgcacagct cgcggcagag ctcggcgtcc gcgcccgagt ccgcgcgcag tccgtaatcg
     3241 acgtgcagcg cgctcacctc cgcccccagc cgcaccgtca catccagcag gcagacggag
     3301 tcgccgccgc cggagagcat cacgagcagc ggctcacccg cgcggatcag accgctgcgg
     3361 cgcgcggcgt cgagcgcgcg ttccacgtca ctcccggccg cggacgcggc agagccggcg
     3421 cggctcgtcg aaccccttga gcttcacctg gccgatgttc tcgaactcga ggtgctcgtc
     3481 ctccctgatc tcgtccatca ccgcgtcgct caccaccacc tcgccgccgc gcgcccgcgc
     3541 gaccacgcgc gcggcgaggt tcacgtcgcg cccgtagtag tcaccgtcgc gatagagggc
     3601 gacgccagcg tggatgccga tgcgcggggt cgggcgctcg tcccagaggc gctggaaccc
     3661 gacgctccag tcggtcagcg cgccagggtc ggtggcgacg atcatcacct cgtccccgat
     3721 cgtcttgatc acgcgcgcgt cgtcgggaag cgtctcggtc acggcgtcga tgaagcgctc
     3781 cacagtcgtg agcgcctcct cctccccggc ctcctccgtg aagcgcgtgt agccggccag
     3841 gtcggcgaac gccaccgtca cgcgcacgcg acccaggtcg atcgactcgt cgagctcgac
     3901 ctccatgtgg cctaccacgt cgcgctcgac gaactcggcg aggaagcgct ggtgcaggta
     3961 gtccatcagc ggcgaggcga gcggcagcag gtcgcccgcg aggtcctcca tctcctcggc
     4021 catctggagg ccgggcacgc cctggcgcat gagcggctcg tggacgtaga tgtggaacag
     4081 gcgcgtctcg gcgtcggcga tccgggcgag cgactggccg tagacgcgca ggagctggag
     4141 gaaggcgacg agcgggaagc cggcgtccag gacggaggcg acgtagcgca gcgccttgag
     4201 gtcctcgtcg gagatggtct cgggccggcg cggggcgaag ccgagcgtgt tccagatccg
     4261 ctcgaccatc gacggctcga gcccgacggc gtcggcggcc tcctcgaacg ggatcgagcc
     4321 gcggttctcc gggacgagca gcgcctccac gtacccgtgc gcgagccgcc cctcctcgcc
     4381 cgccttgcgc aacgcctgga gcgtgtgccc gcgctcgcgc agccgggcga ccatacgcgc
     4441 gtgcgccacg gccgccggcg tccactcggc ggagccgtcg tgctcgggga tcacgccggt
     4501 ctgcgcccag cgcctgagcg tcgcctgggt gacgccgacg gagtcggcgg cctcgcggag
     4561 gctgaggagc cgctcgctca tgacagccac gctacccggc cataggtaat gtgcgcggcc
     4621 cagcacctag ggagggtcca ggagtgagtt acttcgtcac cggcgcaacg ggcttcatcg
     4681 gccggcatct tgtcgagctg ctgctgaagc gcgagggcac gatctacgcg ctcgtgcgcg
     4741 agggatcgcg cgggcgcctc gacgagctag cccagcgcct tgacgccggc gaccggatcg
     4801 tcccggtggt gggcgacctg accaaggagg gcctcggcgt cgaggggttc gacgagcgga
     4861 tcgaccactt cttccacctc gcggcgatct acgacatggc cgccgacgag gagacgatgc
     4921 tcaagtcgaa catcgagggc acgcgccacg tcgcccagtt cgcgaactcg atcgacgtcg
     4981 gcaccttcca ccacacgagc tcgatcgcgg tggcagggaa gttcaggggc ctgttccgcg
     5041 aggacatgtt cgacgaggag cagaagctcc cgcacgccta ccaccagtcg aagttcgagt
     5101 ccgagcgggt cgcgcgcacc gaggtgaagg cgccgctgcg ggtgtaccgg ccgggcatcg
     5161 tcgtcggcca ctcgctgacc ggcgagatgg acaaggtgga cgggccgtac tacttcttca
     5221 agctgatcca gcggatccgc ggggcgctgc cgcagtggtt cccgatcgtc gggatcgagg
     5281 gcggcaaggc gaacctcgtt ccggtcgact tcgtcgcgcg cgcgatggac cacatcgcgc
     5341 acgtggacgc cgacgacctg ccctgccaca cgttccacct ggtcgacccg aacccgctga
     5401 ccgtcgggca ggcgatgaac gagttcgcgc gggccgccca cgcgccgcag ttcgcgatgc
     5461 ggatggactc gaacatcacc gacgtggtgc ccaagccggt gcgcgcggga ctgaacgcga
     5521 tcccgaccgt gaagaacatc cgcgagacgc tgctgcgcga tctgcacatc ccgccggcgg
     5581 cgctcgagaa ccgcgagttc gaggccgact tcgacgcgcg cgagacgcag cgggcgctgt
     5641 cgggcaccgg catcgcggtg ccgccgctgt ccacctacgc ggcgaagctg tgggactact
     5701 gggagcgcaa cctcgacccc gacctgttcc gcgagcgcag cctcaccaag tcgatcgagg
     5761 gcaagcgcat cctcgtgacc ggcgcgtcga gcggcatcgg gcgcgacctc gcgatgaagc
     5821 tcgccgacgc cggcggcgag gtgatcctcg tctcgcgcac gcgcgagaag ctcgaggagg
     5881 tcgcggacgc gatgaggaag gccggcggga acccgcacgt gcacccggcc gacctgtccg
     5941 acctcgagga cgtcgagcgg ctcgcgaacg aggtgatcga ggagtacggc ggcatcgaca
     6001 tcctcgtgaa caacgccggc cgctcgatcc ggcgctccct gaaggcgtcg cacgagcgct
     6061 tccacgacta cgagcgcacg atgcagctca actacttcgg cgcgctgcgg ctgatcatgt
     6121 cgttcatccc gggcatgcgc gagcgccggg cgggccacat catcaacgtc tcgagcatcg
     6181 gcgtgcagac gaacacgccg cgcttctcgg cctacgtggc gtcgaaggcc gcgctcgacg
     6241 cgttctcgcg ctgcgtggcg ccggaggtga tcggcgacgg cgtgcacatc accacgatct
     6301 acatgccgct cgtgcgcacc ccgatgatcg cgccgaccaa catctacgac gcgttcccca
     6361 cgctgtcgcc cgacgaggcg gcgcagctca tcaccgacgc gatgatcgac aagccgaagc
     6421 gggtcgccac gcgcctcggg acgttcggtc agatcctcta ctcggtctcg ccgaaggtcg
     6481 tcgaccaggt gatgaacacc gcgtacaaca tcttccccga ctcgaaggcc gccaagggcg
     6541 acaagaagcc gagcgccgag gcatcggggg acggcgacgg cgcgcgcgac ggcggcaggg
     6601 acgagaagca ggagatgtcg accgaggcga tcgccatggc ctacctgctg cgcggcgtcc
     6661 acttctagag cacggcgcgg tattgcgcca tcttcgcgga agccgagtta gggacactta
     6721 tcggtaaggc ctcctcgaaa tgaggtgggg tcagaaatga gacggggtgg tcaggggccc
     6781 gcaacggacg agtaaccccc ccgcatcggg gggatcggct gaactgcgga accaggtagc
     6841 ggtacagcga cgtttaagtg ctcgtcccgc taaggggagt acattccggt cggccccgct
     6901 tgaccactcc aatcgacatc accgacccgc ggctcgtcaa ggcctacgcg cacccgctga
     6961 ggatcgagat cctcagtttg ctcgacaacc gggtggccag tccgaagcag atcgccacgg
     7021 agctcgagac gccgctcccg aacaccgcgt accacgtgcg gcagctcgcc gcgctcggtc
     7081 tggtcgagct cgtgcggcgc acggtgcgcg gcggcgcggt cgagcaccac tacacgacaa
     7141 aggtccggcc gacgatcccg gacgagctct ggtccacgct gcccgacata gtgaggcgcg
     7201 ccgccatggg aggagccgtc cagcaggcgc tctcggagat gatccgggcc gtcgaccagg
     7261 gcgggttcag ccctgacgaa gcccacctga cgcgaacgcc cgcgcgcctc gacgcaaagg
     7321 gatggcgagc ggtctcgcgt gagatgcaca gggcgctgaa gcgcgtggac gcgatcgtgg
     7381 cggagaccga ggcgcggctc gagaacgatc ccgaagagga gaccgtcgag gcgatggccg
     7441 tgatggcgct cttcgagcgg ccggagatgg aacgcgacgg cgcggccggc gctccgcggc
     7501 ggaccgggcg caccccggcc caggcgggca ggcgcttcag ctaggcagcc aggtgcgggt
     7561 agtcgcgctc gaccagagcg cggtactgct cgtagaccgg cttcgttatc gggatcagcg
     7621 cgagcgcctt cttcacgttc cccgaggtct tgatgcggcg gcgggcgatc gccatcgcca
     7681 cgttctcctt gccctggaag tagcgattcg cgacgtctga gtccatggtc atcttgacct
     7741 cgggctccca gtccacgtcg tcgctccact cccagcgcag gtgagcgccg tcctcgccgg
     7801 cgtcgctcgc gggcgcgacg ttgactacca ggtcgaggtc cgggaactcg aaccgctgag
     7861 gcgtgtcggc gtcgtgcagc ttcggcccca tgtcggggtc cgtgctcatc atctcgaaca
     7921 ccctgtccat gacctcacgg cactcgcgag ccgagttgaa gctcatgccg ccaccctaaa
     7981 ggaacgcgga ccggcgcttg gcgaggtttt cccagacctt cttctggatc acgtcgcgga
     8041 cgtgctccga gagctcgaac acgagcgtgc ggtcggccgc ggcgtccggg ccgaactcgg
     8101 aaaggtcgat cggctcgcag aactccatac gccagcgcga cgggagcggg accgcgccga
     8161 gcggccccag tgcggggaac gtcggcgtga cggggaagaa cggcgtgccg gcgagccgcg
     8221 cgagcagcgg tgcgtcggcg agcttgggat ggacctcctc gctgccgacg atcgcgaccg
     8281 gcacgatcgg cgcgcgcgtg cgcagtgcga tctccacgaa gccgccccgg ccgaagcggc
     8341 ggagctggta gcggtcgcgg tagagcttct gcgcgccgtt cgtcccctcc gggaagaccg
     8401 ccaccagctc ctcctgatcg agcagccgca gggcgttgta cggggaggcg gggaccccgc
     8461 ccagcttgcg gatcgcgacc gagacgtagg gcagctcgaa cgcccagtcg agcaccgtga
     8521 agcgcgggta gcgcgggagg gggtgctcgc gctggagcgc gacgccgatc atcgcggcgt
     8581 cccacggcag gatcccggcg tggttggcca cgagcagcgc ccgcccgtgg ctcggcacgt
     8641 tcgtcacgcc ctccacctgg atgcgccacc agcggtcgta gaggaactcg aggaacggca
     8701 gcgccgcctc gacgaactcc tcgtcgagcc cgaagtcgtc ctcctcgtac tcgccgccga
     8761 gacgccgcat gaccgcctcg aggctctcgc gcgcctcgcg cggcaggccc atcgccgcgc
     8821 cggcgaaagc cgtcgccgga gccgcaccgc cctcgagccc gccgcgcaga gcgcgcagga
     8881 acgagacggc ggggccgagc gggccgtcgc tcacgccgcg gccgccgtgg agtcgcgtgc
     8941 ggcggtgtag tcgcggacgg cctcgaccgt cgagaagcgc ggcgtgaagc cgacctcctc
     9001 gactagccgc gttgtgtcca ccccgcgccc gtagcgcagc agccgccgga agtcggggga
     9061 gaagctgaac aggccgaggc ggcggccgag ctggctggtg gtgccgaaga gcggggcggg
     9121 caccggcatc gtcggccgcc tgacgagccg tatcgcgcgc gtgagaccca gcgtcccgcg
     9181 ggcggcgacg ttcaccgcgc cgcgcaccgg attgcgcacc gcggcgacga acgcgtcgag
     9241 cgcgtcgcgc tcgtggatga gctggatgcg ggggtcgaag ccgaggtagg tcgggacgac
     9301 cggcagcgag aggtagcgcg tgatctgcgt cgcgtgggcg gggccgatgg tgagctggta
     9361 gcgcagcatc gtgcagacca cgtcctcgtg gcggcggcgg aaggtctcga agaggttctc
     9421 gatctcggcc acgtcgcgct ggaagcgcgt gcgcagcggg tagaggcgcg acatgtcctc
     9481 ggtgaagaac tggggcgccg ccggctccga gccgtagatt ccggccgagc cgcgcaccac
     9541 gatcgtccgc agcgtctcga tgcgctcgca cgccgccagg agctggagcg agccgatcac
     9601 gttgatgtcg tgcgccgcgc gcgcggacat tccgggcccg ggctggcgga cgatctggtt
     9661 gtggacgacc gtgtccaccc cggtgggtgg aatcaaccgg cctatctccg gatcgcggat
     9721 gtccgcgtcg atgaactccg tgaaacgcag cggggcgcgc gggcgcttgg tgtcgatccc
     9781 gacgacgtac tcgaccccgg ggtcgtcctc gagccggcgg gccagctcgg tgccgaggta
     9841 gctggcgatg ccggtgatga ggatgcgccg gccgggcacg tcaggagcgg ccgctgcgcg
     9901 agccgccgcc gcgcttggcg ggggcggcgc gcttggcgcc gccgcccctg gccggcggct
     9961 tcgccggcag gcgctcctcg atcgcgtc
//
LOCUS       DASVPL010000273        26123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_111290, whole genome shotgun sequence.
ACCESSION   DASVPL010000273 DASVPL010000000
VERSION     DASVPL010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 26123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26123
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111290"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VF517_15135"
     CDS             complement(<1..737)
                     /locus_tag="VF517_15135"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8054321.1"
                     /translation="MRTALWLALAAAVLAASPAPAGAAPPPVKHVFVLLLENENADST
                     FGADSKAPYLAKTLRAQGAFVPNYYATGHLSLDNYISLVSGQGPNPFTQADAPFFIDF
                     VPGTPAPDGQFLGSGAVYPSQVKTIADQLDAKGLTWAGYMEDMGNSDTEPKTCRHPAI
                     GAQDRTQSARVGDQYAVRHNPYMYFHSIIDDQAKCDARVVPLDRLPAALASESATPNY
                     TFITPNLCHDGHDEPCVDGQPGGMASA"
     gene            complement(755..1960)
                     /locus_tag="VF517_15140"
     CDS             complement(755..1960)
                     /locus_tag="VF517_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8054322.1"
                     /translation="MAVADPYLEKTDEQRAITEMVRQFVDEQVIPIAEEHDHEDKFPE
                     AVVEQMKELGLFGVTIPEEYGGMGLDLTTYAMIVEELSRGWISISGIVNTHFIGSYLL
                     MKFGTDDQKDRFLPRMATGEIRAAFSLSEPGLGSDVQAITTRAKQDGEAWVINGQKMW
                     VTNGLLSGVVFLLAKTDPEADPPYKGMTCFITEKEPGVAENTGDYKGLDIPPKIKKMG
                     YKGVESTELVYADYRCPTQNILGGEEGLGKGFVQMMDALEVGRVNVAARGVGIAQRAL
                     ELGLRYSQERKTFGKEIAKHQAIQFKLADMATKVEAARLLTMKAARMKDAGQRSDLEA
                     GMAKLFASETGKEVVEDSFRIHGGYGYSKEYEIERLYRDAPLLLIGEGTSEIQRMVIG
                     KKLLERHKI"
     gene            2019..2918
                     /locus_tag="VF517_15145"
     CDS             2019..2918
                     /locus_tag="VF517_15145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8054323.1"
                     /translation="MPEQAEASRTAAYMALFRALETGRRGGLFADPYAVRFLPGGLRA
                     AGAAARVPPLRAGVCRYIDRRWPGARTSAVARTRAIDDLLAEALGTGLRQVVFLGAGY
                     DTRALRLPSLAGVAVFELDQPALLAAKRERLGADAERTGPATARALRRVPIDFARDEV
                     VPTLARAGFDPASPAVFVWEGVTNYLTAEAADSTFRAVASTARGGRLAFTYVHSGVID
                     GSVEFEGAERLRSTVSGVGEPWTFGLHPDELAAYLADRGLRLLGDEGADDYRRRLLGD
                     SPHVLRGYAFYRLAVAEVAPSDA"
     gene            2964..3710
                     /locus_tag="VF517_15150"
     CDS             2964..3710
                     /locus_tag="VF517_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054324.1"
                     /translation="MEGRSKRSDGSGGSVSGFVGTLPAYLGMSLAAIYALGALEARGQ
                     LSESGVRTLDALPVIPVEQLLARGIWAVTEPLPLVLAVVLVTQGLAALFFRVREYRGR
                     EDTTAGLLKGMALAIALLAIALAMMPPAMIPRVLFAGVGYGGVALVLGLRHDWRPESL
                     RFVAVTGSVFAVIFAMTGTYFAPDPLPDASIQTESSVVRGRLIAATDATWYLADRGRV
                     RAIPANHVRSAEVTKRDLHRSESLLRSLLD"
     gene            complement(3745..4293)
                     /locus_tag="VF517_15155"
     CDS             complement(3745..4293)
                     /locus_tag="VF517_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054325.1"
                     /translation="MLTRFHTSLLAAFLAAVCLLPGSALAQSTGGAQYQPPASKAKVV
                     NGKAIAPSDAPPEVKAAIAAANRIVTKPYKYGGGHAKVEDTGYDCSGTVSYALLKAGL
                     LTGSPLDSTGFMGWGAPGKGKWITVYANGGHAFMMIAGLRLDTGYRDKYVAKWGGAPG
                     TGPRWGKSRSANGFTVRHPAGF"
     regulatory      complement(4290..4427)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            4607..6022
                     /locus_tag="VF517_15160"
     CDS             4607..6022
                     /locus_tag="VF517_15160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8054326.1"
                     /translation="MNFARDVVEAAAPGRTALVALARDGSRREIAFGEVADRSARLAG
                     ALAARGVSRGDVVMTVIGNQPDWVLALVACFRIGAVALPATEQLRPKDLRARIDATRP
                     AVVLADPRNAQTVADSGYDGHLTTVADELRTDPVPAPAAELSPTDPALITFTSGTSGE
                     PKPIRHAQRYLSGQAVQAEHWLGARPGDLVWCTAASGWSKSARNVFIAPWLRGASALL
                     HDARFDPDERLALVERERVNVLCMAPTEYRVMAKRTELRPVESLRHLVAAGEALDPEV
                     VRVWREASGLEIHDGYGQTETGSLTGMPIGPPVRPGSMGLPLPGFRLWIEDGELCADP
                     ATIPTFFLDGGEGTWRTGDRVAQDEDGYLWFEGRADDVIISSGYRIGPFEVESALVSH
                     PAVAEAAAVAAPDAERGSIVRAIVVLRDGEEPSDQLARALQDHVKAETAPYKYPRIVE
                     FAGSLPKTASGKIRRAELRGD"
     gene            complement(6019..6642)
                     /locus_tag="VF517_15165"
     CDS             complement(6019..6642)
                     /locus_tag="VF517_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX8054327.1"
                     /translation="MTALVIGFGLGFAVAAQVGPVTLLAVRSVIRGGLAVGIAMAAGV
                     AIVDAFYAGAGAAGAGAALELEPLRVAFGILGALVLAAIGARTLWSAFRVRLGGETEE
                     EVGTPGRAWRTALAATASNPLTIVSWAAIFAAASTAELAASPAAAALMVAGVLAGSFA
                     WMAALALVLTLVRRRIGDRGLRILDAASGTGILVFAGLLGWRTLREG"
     gene            6714..8666
                     /locus_tag="VF517_15170"
     CDS             6714..8666
                     /locus_tag="VF517_15170"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8054328.1"
                     /translation="MPNRLADETSPYLLQHKDTPVDWHPWGEEALTRAREEDRPILLS
                     IGYSSCHWCHVMERESFENDEIAAVMNEHFVPVKLDREERPDLDAIYMEACVAMNGHG
                     GWPLNVFLTPEQVPFYAGTYFPPEEGRGLPSWRRVLEAVARAWDTERDKIREGGERTA
                     QRLAGGARLKPTDELMDPKRLDEAVAVLRGSFDARYGGFGRAPKFPPSSAIEFLLRRG
                     EREMTTQTLWAMASGGMYDQVGGGFARYSVDERWLVPHFEKMLYDNGLLARAYLHAWQ
                     ATGEELFREVACETLDWALREMRAPEGGFYSALDADSEGEEGKFYVWTLDELRSVLGD
                     DADAAIDYFGADHGGNFEGTNILTRGSDPPERLPEIKSKLYGVRKERVWPGLDDKRLT
                     SWNALMISALADAGAALERQDYLDAATACADFVLRELRDTEGRLLRTFKDGQAKLNAY
                     LEDHAYLVEALISLYEATFDPRWFRAARETADAMIERFADDENGGFFETSIDHERLLT
                     RRKDLEDNPIPAGNSSAAYALLRLAALTGEHEYEQRAVSVFRLLHHVAPQHPQAFAHL
                     LQALDFHFASVKEVALVGDDRAPLERVVRSGFRPHIVLAGGDPDGVPLLAERGPVDGR
                     AAAYVCENFACQRPVTEPAELEKLLA"
     gene            8663..9055
                     /locus_tag="VF517_15175"
     CDS             8663..9055
                     /locus_tag="VF517_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054329.1"
                     /translation="MSTRLPWTEALASWQPAAIVERLTDDAVIRVAVHDAPMQGREVA
                     EFLFGVLSEELAAPRVTDEIVEGDRAVVLFETEIRGQAAQGLNVVRHAPDGRVADLTV
                     FFRPLESLATIAEVVGARMAERFGPPPG"
     gene            complement(9293..10477)
                     /locus_tag="VF517_15180"
     CDS             complement(9293..10477)
                     /locus_tag="VF517_15180"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8054330.1"
                     /translation="MSVRLLVAAVLATLALASPAHALPDCSPLPEKRTLLTGQGQLES
                     IISDARGRIFYSDIQNGGRLFRLDAPGAEPKQLVQGINGTGGLAWDTDGSLVLGFNGG
                     NQNAVADGTAGGLLHVNPETGETAPLASGMGMANGIVRGPDGAFYASNDFAGGIDRVK
                     DGKVEDDWSKVQTPNGLVIDTAGRYLYAAQTFKPASVARIDLSNPAKEEPYYEATAPD
                     LQGGPDGMTRDDRDRLFVAVNASGEVWRIDTDRTACRLASGIMNASAVNWGGGAPGFP
                     ARNLYVVGFSGVLVELADATDRPPPAGPPAAKPPRLRLAVEPKRVAAGSSTRWRFAVT
                     AGGRAMPGATVRFAGRRATTNADGRAELTVRIARAGLRKASAALAGYPSARKTVRLLP
                     RR"
     gene            complement(10474..10938)
                     /gene="bcp"
                     /locus_tag="VF517_15185"
     CDS             complement(10474..10938)
                     /gene="bcp"
                     /locus_tag="VF517_15185"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX8054331.1"
                     /translation="MSVEEGAEAPDFELRDQDGEAVRLSSLRGKTVVLYFYPKADTPG
                     CTAQACAIRDRQGEFEGAGAVVLGVSPDPVPALRKFADKYGLPFTLLSDEDHAVAEAY
                     GTWVHKPARGYMKERMGIERSTFVIGSDGRIERVLRNVKAQGHDELVLSGLA"
     gene            complement(10935..12290)
                     /gene="glnA"
                     /locus_tag="VF517_15190"
     CDS             complement(10935..12290)
                     /gene="glnA"
                     /locus_tag="VF517_15190"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX8054332.1"
                     /translation="MADTPQTADDVKALVADRKIRFIRLWFTDILGQLKSFSINAEEL
                     DDAFEGGMGFDGSSITGFNAIEESDMIAMPDASTFAVLPWRPSEGGGGVGRMFCDVVT
                     PDRQPYEGDPRHVLRRALQRAEAMGFDAFNVGPELEFFYFRNSKPESGVPEVLDEGGY
                     FDLTTLDAGSDVRRDTVLALEQLGIHVEYTHHEVGPSQHEIDMRYAPALKMADDCMTY
                     RITVKEYAMKYGWHATFMPKPLFGENGSGMHTHQSLFKEGRNSFYDGDDQYYLSATGK
                     CFIAGQLRHAREISSIFAQWVNSYKRLVPGYEAPVYVAWSRRNRSALVRVPLYHPGKE
                     QATRMELRCPDPACNPYLTFATLLQAGLEGIEKGYELPEPMEQNLYHLSADDRKRRGI
                     EQLPETLGEAIELTAESELVLRTLGEHMFTRYVELKRKEWDDYRVQVSAWELDRYLAV
                     L"
     gene            complement(12344..12673)
                     /locus_tag="VF517_15195"
     CDS             complement(12344..12673)
                     /locus_tag="VF517_15195"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054333.1"
                     /translation="MAVRVTVERRTCCTYYHRAVELIGKRWSGAILAVLLDGPLHFSE
                     LRRAVPDISDRLLSERLKELESEGLVGRRVIDGSPVRTEYSLTAKGQALEPALRTLQA
                     WARSHLR"
     gene            complement(12715..13284)
                     /locus_tag="VF517_15200"
     CDS             complement(12715..13284)
                     /locus_tag="VF517_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8054334.1"
                     /translation="MAKKAKESRFHIHDEMSAPERSVPILKGALSTGGQVSNMLGVLA
                     GAPAVLRAYARFRSELRQGSLPLKSQQRVALGVAQHEGSDYALSMLMRSARDAGLGLD
                     EIGLAREFRSIDRREAALLAFVKGLLEAPDAVPLHLVEEAREAGWEDEQLIEATAHVS
                     LGLFHALITRGGNVPLDGSAELGRVLRAA"
     gene            complement(13498..13827)
                     /locus_tag="VF517_15205"
     CDS             complement(13498..13827)
                     /locus_tag="VF517_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054335.1"
                     /translation="MGEAEHARRRASARDAARPRRRAGERDAVRPALSLVVTLTGEEA
                     PRRDPDTGRRTVTITGQRAAPAAPRRRPDAASRVGPRPDRIAMWAFFMGIFLVGVATA
                     TADAAPL"
     regulatory      complement(13888..14042)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(14101..14589)
                     /locus_tag="VF517_15210"
     CDS             complement(14101..14589)
                     /locus_tag="VF517_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8054336.1"
                     /translation="MAAAAFVLAFLALGVGVLFVAFSGGPTRAREAYLTGGRRAFRFV
                     IPVIYVAIGIAVPVLVIAGRDRAEGGVGDLKDKKPAAQVAQGRDLFRQTCASCHSLAA
                     ANARGVTGPNLDQIGQVTKQRILKAIEIGGTGQGRMPPRLLAGENAEAVGAYLTEVAG
                     RE"
     gene            complement(14638..15096)
                     /locus_tag="VF517_15215"
     CDS             complement(14638..15096)
                     /locus_tag="VF517_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054337.1"
                     /translation="MATTSERTDAHCPHCLLLVEADALPAWPPQPVRCPHCRLLIGAG
                     RARDAGDGAPGARGAAAGVFAREAQRGRDSAEVVSPDEVIEGIRTVAERLGSPPERLL
                     MVDYQQSAAEDDDLPDLTSVFAAFGSWKRARREAALSGDEGELSGDEAAA"
     gene            complement(15181..16227)
                     /locus_tag="VF517_15220"
     CDS             complement(15181..16227)
                     /locus_tag="VF517_15220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8054338.1"
                     /translation="MSEEGLEGRIDDLLRRAEENGYLEFSDVAAAVEGLDVDDDRIEA
                     LYREAERRGIEVLDHNERSGANEARFTHNAVAEATSDSLQLFLRDIAQRPLLTAQEEI
                     ELAKRIERGDQAAKDRMIEANLRLVVANAKRYRGLGLPFLDLIQEGILGLIRAVEKFD
                     HRRGFKFSTYATWWIRQAMQRGVQHQSRTIRIPVHIGQELTKLRTTERKLTAALGREP
                     TEVELAEALGTDPARIEELRSAERVPVSLETPVGGEGEAELGSLLPSDAPSPLEEVAV
                     ELEEESIRRALERLDDTARSVIEMRFGLDGREPKTLREVAKRMGLSPEGVRKLERRAL
                     RKLAEERELQALAV"
     gene            16322..18700
                     /locus_tag="VF517_15225"
     CDS             16322..18700
                     /locus_tag="VF517_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8054339.1"
                     /translation="MRGWFVLGALAVLLALPASAAARVVRAGDILPPGQSGHVTLAGV
                     PSGTGSPHLTDQLERFLAFEFKPHGFDNPAQSIETQRIGVTLERDDWGVPTIRGQNDD
                     DIWFGAGYAVAQDRLFQMELFRRATQGHLAEIVGRSYLEDDIKIRRDFYTGPELDAQV
                     ASLPDNYEARYRSYAAGVNAWIAETRADPNKLPAEFALVNAAPPADWTVRDSAAISVY
                     LARTVPSDDGEEMGNWKALTTFGSKLFDRVLPIRTKGQVPTVPPSEGSFPAQPGRKAK
                     DEAAGRKRSKAFLKSVPMPKEEDVGGTHAAARELGALPMLGQLGQRGSNMWAVRNKNV
                     GATLFNGPQLGFQIPELFVEYEVHRPGYDARGVTAPGVPLVAIGHNGQVAWGFTSGLT
                     DEDDLYVEKLTGPEKYMFEGKERQMECRDETFSYRSPPSDATSILDLEPSPPESGSET
                     RRLCRTIHGPVQGRAEGTAFARRYAIWGREFDTLGGLAGVSESKNVAEVDRAMAKVTW
                     NENLMAADSQGNIGYWHPGLLPIKPRGYDERLPFPGTGEAEWRGFLTPAQRPHSINPK
                     QGWLVNWNNMPSAGWTQGDIPARERGLGPYHRAHLLSKVVARAHANGGGYEQTRAVDR
                     VVGTTAQQRPVAAGPLNSALRGATGNAKTVLETIKRWNGSYHVTDANGTVDPGVAAWR
                     EFANAALKIGFGKLWDSKDLRLFETSAGSSHRQELSNVQVYALRTIGAKGWRQAAKLA
                     FPALAARFGSEDPAKWRDKRLMYNPSAQGAASFDPIPFLDRGTWQQVVELGP"
     gene            18690..20132
                     /locus_tag="VF517_15230"
     CDS             18690..20132
                     /locus_tag="VF517_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEX8054340.1"
                     /translation="MAPRASSRRDLLTPAASKAPVRRRADAVVVGAGFAGLTAARELV
                     RAGRSVIVLEARNRVGGRTLNAPIGGNEVIEVGGQWVGPTQDRLLALGEAMGVPTYKT
                     YNEGDNLYYRGGSLQRFSSTGPLGAVPPDPEGAPQAAAFIQQINDMAARVPRDRPWEA
                     ASAVEWDSQTVETFKQANAPTEGGRFLFDVGVEAVFAAEPRDISLLWLLFYTAAAGNE
                     TTPGDFNRLLNTAGGAQESRYVGGSQRVALRVAKQLGKKRIVLGSPVRRVKQGGKSVT
                     VESDRAVVNAKRVIVAVPPTLCQRIEFAPGLPAARDRLTQNWPMGSVVKCEAVYDKPF
                     WREEGLTGQVVSDASPVKITFDNTPASGTPGVLLGFIEGRESRVWGEKSVGERRAAVL
                     RNFATYFGERALKPARYVEMAWQHETWSGGCYAGNLTPGTLLDFGPAIREPVGRLHWA
                     GTETSTLWAGYIDGAVRSGERAAAEVLARL"
     gene            complement(20157..21119)
                     /locus_tag="VF517_15235"
     CDS             complement(20157..21119)
                     /locus_tag="VF517_15235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054341.1"
                     /translation="MGDLRGSPGGALRGPLPERARRRGGRARAGRRGRLSGEAKPPAG
                     AGPPIAAPSTTAASAAVNPVASSGFGRSARAYDRARPGWPPAAVHAAFDHWSLAPQAA
                     RVVDLAAGTGRLTRELLAAGASVTAIEPLDEMRALISGAETLPGTAESIPLSDAFADA
                     VFVAEAFHWFDAPRALAEIARVLRPGGGLAVLWNVGVTADDEEPWRAEVVELVRDVYY
                     HPEGRRTPSASGNDPRDDPAAWRTGPGWELFEPLTLRSFEHTQRITAEDYVALVSSWS
                     FVGALPDPDRTELLAGVAAVLAEHGVAAFDQRWRTDAYLTSLNS"
     gene            complement(21014..22459)
                     /locus_tag="VF517_15240"
     CDS             complement(21014..22459)
                     /locus_tag="VF517_15240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX8054342.1"
                     /translation="MGELGGFLKIHRVEPNKRPKTERVEDFKEYVVLLPQAGLREQGA
                     RCMDCGIPFCHSGCPLGNLIPDWNDLVYRDRWKEAIEALHATNNFPEFTGRICPAPCE
                     AACTLDINDDSVTIKQIEQAIADRAFEEGWIKPEPPPFRTGRTVAVVGSGPAGMAAAA
                     QLNKAGHSVTLFERNDRIGGLMRYGVPDFKLEKRFVQRRADLMEAEGVEIRTGVNVGV
                     DITADELREQFDAVVLAIGSTIPRDLPVPGRELDGVHFAMEYLEQRNRFVAGDPPPER
                     PISAAGKHVVIIGGGDTGADCLGNSHRERPASVTQFELLPEPPPERPDDRTPWPQWPL
                     VLRTSGAHEEGGERRYSVMTTHLTGEDGRVTHLHGHEVAGPPGFDKVDGTEFTIQADL
                     VLLAMGFLHPQHEGLVDQLGVERDGRGNVSDDDFATSVPGVFACGDARRGQSLVVWAI
                     SEGRQAARSADRYLSGLGGAAGEPELAAAGA"
     gene            complement(22486..>26123)
                     /gene="gltB"
                     /locus_tag="VF517_15245"
     CDS             complement(22486..>26123)
                     /gene="gltB"
                     /locus_tag="VF517_15245"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX8054343.1"
                     /translation="RWQVTSDDFVVLASETGVLEAEPSTILRKGRLQPGKLFFVDVEQ
                     GRVIEDAELKHEIAARKPYGQWFKDCTVHLDDLPAVAPREIPADPVRTRQLMFGYTQE
                     DLRITLARMGGASAEEPIGSMGNDFALAVLSDKSPPLYSYFKQLFAQVTNPAIDPIRE
                     RVVMSLETAVGPQSNLFTETPEHAHQLIIGQPILSSGELEKLRQVDHDVFAAHTLDAT
                     WPPADGPEGMARAMERLCEEASERLAQGDNILILSDRAAGEDRVPIPALLAVSGVHHH
                     LVREGTRLQTGLVVESGEPREVHHVCCLLGYGASAINPYLAFETLHDMFDDGLLPKVE
                     SAADAEKHFVKAMEKAILKTISKMGISTVRSYSGAQIFEAVGLERGLIDKYFTGTTSR
                     IGGIGIDVLARETMERHRRAYPRSSHRLPVGGVHMWRRDGEFHQWNPDTVAKIQHAVR
                     HGGQDSYEEFTKMINEQSKRRATLRGLLDVRDPGEPIDIEEVESAKDIVKRFATGAMS
                     LGSLSAEAHETLAIAMNRLGGKSNTGEGGEDPRRYTPDANGDSRRSAIKQVASGRFGV
                     TAHYLVNADELQIKVAQGAKPGEGGQLPGHKVDEYIAKIRHSTPGVGLISPPPHHDIY
                     SIEDLKQLIFDLRCANPTARISVKLVSEVGVGTVAAGVAKANSDHVVIAGHDGGTGAS
                     PVSSIQSAGVPWEIGLAETQQTLVMNKLRDRIVVQTDGQMKTGRDVIIGALLGAEEFG
                     FSTAPLITMGCIYMRACHLNTCPVGIATQDPELRKRFQGQPEHVVNFFFFIAEEVRAY
                     MAQLGVAKFEDLVGRTDLLEPDAAIDHWKARGVDLSGLLAPPQAPADVPRRCVRKQDR
                     VLDDHLDHELMRLAEPALERGEKVSFSVSAENQHRCVGGYISGEIARRYGIDGLPEGT
                     IDVQLEGSGGQSFGGWLAPGVTLTLTGDANDYTGKGLSGGVLTVRPPADVPFAAEDNV
                     VIGNTVLYGATAGRAFFRGLAGERFCVRNSGAHAVVEGVGDHGCEYMTGGRVVILGPT
                     GRNFAAGMSGGVAYVLDRDYEFASKCNTEMVELEHPDEDDFAEIRALVEEHRERTGST
                     VAERVLADWDSLTSAWVKVMPMDYKRVLRERQERQRIEAGEPAIVAEHGDGDGAGPVR
                     VGQGSPYPVADGDQETTETGEAGQTEDAADAGARAERRAEAAGAGEAAADQAAAAAEE
                     EVLPHGG"
BASE COUNT         3538 a         9290 c         9467 g         3828 t
ORIGIN      
        1 ttcgcgctcg ccatcccacc gggctgaccg tccacgcacg gctcgtcgtg gccgtcgtgg
       61 cagaggttcg gggtgatgaa cgtgtagttg ggagtggccg actcactggc cagagccgcc
      121 ggcagccggt cgagcgggac cacccgcgcg tcgcacttcg cctggtcgtc gatgatcgag
      181 tggaagtaca tgtacgggtt gtgacggacc gcgtactggt cgccgacgcg cgcgctctgc
      241 gtccggtcct gcgcgccgat cgccgggtgg cggcaggtct tcggctcggt gtccgagttg
      301 cccatgtcct ccatgtaacc ggcccatgtg agccccttgg cgtcgagctg gtcggcgatc
      361 gtcttgacct gcgacgggta gacggctccg ctcccgagga actggccgtc cggagcgggc
      421 gtgcccggta cgaagtcgat gaagaacggc gcgtccgcct gcgtgaaggg gttgggcccc
      481 tggccgctca cgagcgagat gtagttgtcg aggctgaggt gcccggtggc gtagtagttc
      541 ggcacgaacg cgccctgggc ccgcagggtc ttcgccaggt agggcgcctt ggagtcggcg
      601 ccgaacgtgg agtcggcgtt ctcgttctcg agcaggagca cgaagacgtg cttgaccggc
      661 ggcggcgcgg ccccggccgg cgcggggctg gcggccagca ctgcggcagc cagggcgagc
      721 cacagcgctg tgcgcatcct cggagcctcc cgcctcagat cttgtgccgc tcgagcagct
      781 tcttgccgat caccatgcgc tggatctcgg aggtgccctc gccgatcagg agcagcggcg
      841 cgtcgcggta gaggcgctcg atctcgtact ccttcgagta gccgtagccg ccgtggatgc
      901 ggaacgagtc ctccacgacc tccttgccgg tctccgaggc gaacagcttc gccatgccgg
      961 cctcgaggtc ggagcgctgg ccggcgtcct tcatgcgcgc ggccttcatc gtcaacaggc
     1021 gcgcggcctc gaccttcgtg gccatgtcgg cgagcttgaa ctggatcgcc tggtgcttgg
     1081 cgatctcctt gccgaacgtc ttgcgctcct gcgagtagcg caggccgagc tcgagcgcgc
     1141 gctgggcgat gccgacgccg cgcgcggcga cgttcacgcg accgacctcg agcgcatcca
     1201 tcatctgcac gaagcccttg ccgagcccct cctcgccgcc gaggatgttc tgcgtcgggc
     1261 agcggtagtc cgcgtacacc agctcggtgg actcgacgcc cttgtagccc atcttcttga
     1321 tcttgggtgg gatgtcgagg cccttgtagt cgcccgtgtt ctcggcgacg cccggctcct
     1381 tctcggtgat gaagcaggtc atgcccttgt agggcgggtc ggcctcaggg tcggtcttgg
     1441 cgagcaggaa gaccactccg gagaggagcc cgttcgtgac ccacatcttc tggccgttga
     1501 tgacccacgc ctcgccgtcc tgcttggcgc gggtggtgat cgcctgcacg tccgagccga
     1561 ggcccggctc ggagaggctg aaggccgcgc ggatctcgcc ggtggccatg cgcggcagga
     1621 agcggtcctt ctggtcgtcg gtgccgaact tcatcagcag gtaggagccg atgaagtgcg
     1681 tgttgacgat gccggagatc gagatccagc cgcgcgagag ctcctcgacg atcatcgcgt
     1741 aggtggtcag gtcgaggccc atcccgccgt actcctcggg gatcgtgacg ccgaacagcc
     1801 cgagctcctt catctgctcg accacggcct cggggaactt gtcctcgtgg tcgtgctcct
     1861 cggcgatcgg gatcacctgc tcgtcgacga actgccgcac catctcggtg atcgcgcgct
     1921 gctcgtcggt cttctccaga tacgggtcgg cgactgccat ctggctcctc ggtcggcggg
     1981 gattggctcg tgattgactc gtgagtcagt ctatctccgt gcctgagcag gccgaggcga
     2041 gccggaccgc cgcctacatg gcgctcttcc gagcgctgga gacggggcgg cgcggcgggc
     2101 tgttcgcgga tccgtacgcg gtgcggttcc tgccgggcgg gctgcgggcg gcgggggcgg
     2161 ccgcccgggt gccgccactg cgcgccgggg tctgccgcta catcgaccgg cgctggccgg
     2221 gagcgcggac ctccgccgtc gcgcgcacgc gggcgatcga cgacctgctc gcagaggcgc
     2281 tcggcaccgg cttgcggcag gtggtgttcc tcggcgccgg gtacgacacg cgggcgctgc
     2341 ggctgccgtc gctggcgggc gtggccgtct tcgagctcga ccagccggcg ctgctggccg
     2401 cgaagcgcga gcggctcggc gcggacgccg aacgcaccgg gccggcgacc gcccgcgccc
     2461 ttcgccgcgt cccgatcgac ttcgcccgcg acgaggtcgt gcccacgctc gcgcgcgcgg
     2521 gcttcgatcc ggcgtccccc gcggtcttcg tctgggaggg cgtgacgaac tacctgacgg
     2581 cggaggcggc ggattcgacg ttccgcgcgg tcgcgagcac ggcgcgcgga ggccggctcg
     2641 ccttcacgta cgtgcacagc ggcgtgatcg acgggagcgt cgagttcgag ggcgccgagc
     2701 gcctgcgctc gaccgtctcg ggcgtgggcg agccgtggac cttcgggctc cacccggacg
     2761 agctggcggc ctacctggcc gaccgcgggc tgcgcctcct cggcgacgag ggagccgacg
     2821 actaccgccg ccgcctgctc ggcgactcac cgcacgtgct gcgcggctac gcgttctacc
     2881 ggctcgcggt ggccgaggtg gctccaagcg acgcctgagt atgtgctcac cgtctgaccg
     2941 cgaccatatg ttggcgcgcc acgatggaag gtcgctcgaa gagatccgat gggtcgggtg
     3001 gctcggtctc tggtttcgtc ggaaccctgc cggcgtacct cgggatgtct ctcgcggcga
     3061 tatacgcgct gggagcgctc gaggcgcgcg gacagctgag cgaatccggc gtccgcacgc
     3121 tcgatgcgct cccggtgatt cccgttgaac agttgctggc gcgggggatc tgggcggtca
     3181 ccgagccgct gccgctcgtc ctcgccgtcg ttcttgtcac ccaggggctc gccgccctgt
     3241 tcttccgcgt ccgggaatac cgtgggcggg aggacaccac ggccggtctg ttgaagggga
     3301 tggcgctcgc gatcgcattg ctcgcgatcg cgctcgcgat gatgcccccg gcgatgatcc
     3361 cgcgggtgct gttcgcgggg gtggggtacg gcggcgtcgc cttggtcctg gggctgcgcc
     3421 acgactggcg cccggagtct ctccgtttcg tggcggtcac cggcagcgtc ttcgccgtca
     3481 tcttcgcgat gaccggcacc tacttcgcac ccgaccctct gccggacgcg agcattcaga
     3541 cggagtcctc ggtggtccgc ggcaggctca tcgcagcgac cgatgcgacg tggtacctcg
     3601 cggaccgggg gcgcgtccgc gcgatcccgg ccaaccacgt ccgtagcgcg gaggtgacga
     3661 agagggacct ccaccgatcg gagagtctgc tcaggagcct tctggactag agcccgcgcg
     3721 acgtccggcg gcggaggtgg ccggctagaa cccggcgggg tgccggacgg tgaagccgtt
     3781 cgcgctgcgc gacttgcccc agcgggggcc ggtcccgggg gcgccgcccc acttcgcgac
     3841 gtacttgtcg cggtagccgg tgtcgagccg gagcccggcg atcatcatga aggcgtgccc
     3901 gccgttggcg tagacggtga tccacttgcc cttgccgggc gcgccccagc ccatgaagcc
     3961 ggtggagtcg agcgggctgc cggtcagaag gccggccttc agcaacgcgt acgagacggt
     4021 gccggagcag tcgtagcccg tgtcctcgac cttcgcgtga ccgccgccgt acttgtaggg
     4081 cttggtcacg atccggttgg ccgcggcgat ggcggccttg acctccggcg gagcgtcgga
     4141 gggggcgatg gccttgccgt tcacgacctt ggccttcgac gccggcggct ggtactgcgc
     4201 gccgcccgtg gactgggcaa gggcgctccc cggcaggagg cagacggccg caaggaatgc
     4261 ggcaagcagc gatgtatgga agcgagtcag cattctcttt cgtacgcctg cggggttagc
     4321 tgacgggcta gcgctaaaag agctcgcgcc tctcctccga agagaagact cgccccaaca
     4381 acctggttcc cccgctccgc acttcgcgga ttcggcgtgg tcggcaccgt agcagacggt
     4441 ggcaacggaa ctttcaatcg gcgttatgtc ccgcgacctt gagtctttaa acgcggaaag
     4501 cgctttctag agccaaacgt tacaagttat ggcgcatcgg ataacgcgtg taccccatgc
     4561 aggcccgcct acgatcagcg cccgtgacac cgatggtgca gaacggatga acttcgcccg
     4621 cgacgtggtg gaggccgccg cgcccggccg cacggcgctc gtcgcgctcg cccgcgacgg
     4681 atcccgccgc gagatcgcgt tcggggaggt cgccgaccgc tccgcgcgcc tcgcgggcgc
     4741 gctcgccgcg cgcggggtaa gccggggcga cgtcgtgatg accgtgatcg gcaaccagcc
     4801 ggactgggtc ctggcgctgg tcgcgtgctt ccggatcggc gccgtcgccc tgcccgccac
     4861 cgagcagctg cgcccgaagg atctccgcgc gcgcatcgac gcgacccgcc ccgccgtggt
     4921 cctcgccgac ccgcgcaacg cgcagaccgt cgccgacagc ggctacgacg gccacctcac
     4981 caccgtcgcc gacgagctgc gcaccgaccc cgtcccggcg cccgcggccg agctctcccc
     5041 caccgacccc gcgctgatca ccttcacctc gggcacctcc ggcgagccga agccgatccg
     5101 ccacgcgcag cgctacctga gcggccaggc cgtccaggcc gagcactggc tcggcgcgcg
     5161 ccccggcgac ctcgtctggt gcaccgcggc gagcggctgg tcgaagtcgg cccgcaacgt
     5221 cttcatcgca ccgtggttgc gcggcgcgag cgctctcctc cacgacgcac gcttcgaccc
     5281 ggacgagcgg ctcgcgctgg tcgaacgcga gcgcgtgaac gtgctctgca tggccccgac
     5341 cgagtaccgc gtgatggcca agcgcaccga gctgcgcccg gtcgagtcgc tgcgtcacct
     5401 ggtggccgcg ggcgaggcgc tcgaccccga ggtggtgcgt gtctggcgcg aggcctccgg
     5461 cctcgagatc cacgacggct acggccagac cgagaccggc tcgctgaccg gcatgccgat
     5521 cggcccgccg gtccggcccg ggtcgatggg cctgccgctg ccgggcttcc ggctgtggat
     5581 cgaggacgga gagctgtgcg ccgacccggc cacgatcccc acgttcttcc tcgacggcgg
     5641 cgagggcacc tggcgcacgg gcgaccgggt ggcccaggac gaggacggct acctgtggtt
     5701 cgagggccgc gccgacgacg tgatcatctc gtcgggctac cggatcgggc ccttcgaggt
     5761 ggagtcggcg ctcgtctccc accccgcggt ggccgaggcc gcggcggtcg ccgcccccga
     5821 tgccgagcgc ggctcgatcg tgcgcgcgat cgtggtgctg cgcgacggcg aggagccgag
     5881 cgaccagctc gcgcgcgcgc tccaggacca cgtgaaggcg gagaccgcgc cgtacaagta
     5941 cccgcgcatc gtcgagttcg ccggctcgct cccgaagacc gcgagcggga agatccgccg
     6001 ggcggagctg cgcggggact agccctcgcg gagcgtccgc cagcccagca gcccggcgaa
     6061 cacgaggatg cccgtgcccg aggcggcgtc gaggatccgc aggccgcggt cgccgatccg
     6121 ccgccgcacc agcgtgagca cgagggcgag cgcggccatc cacgcgaagc tgcccgccag
     6181 gacgcccgcg accatcagcg ccgccgcggc cggactggcg gccagctcgg ccgtcgaggc
     6241 cgccgcgaag atcgcggccc acgacacgat cgtgagcggg ttcgacgcgg tggccgccag
     6301 ggcggtccgc catgcgcggc cgggcgtgcc cacctcctcc tcggtctccc cgccgagccg
     6361 tacgcgaaag gccgaccaca gcgtgcgcgc cccgatcgcc gcgagcacga gcgccccgag
     6421 gatcccgaac gcgacgcgca gcggctcgag ctcgagcgcc gctcccgctc cggcggcgcc
     6481 ggcgcccgcg tagaaggcgt cgacgatcgc caccccggcg gccatcgcga tcccgaccgc
     6541 gagcccgccg cgaatcacgg agcggaccgc gagcagcgtg accggcccca cctgcgccgc
     6601 caccgcgaat cccagcccga atccgatgac tagcgcggtc atgccccgac cctacccggc
     6661 gggcgcggtc acccgcgagc tttgcgctcg cgtcttcggg taggtagatg cccatgccca
     6721 atcgcctcgc agacgagacc tcgccgtacc tcctccagca caaggacacc ccggtggact
     6781 ggcacccctg gggcgaggag gccctcacgc gcgcgcgtga ggaggaccgc ccgatcctgc
     6841 tgtcgatcgg ctactcgtcg tgccactggt gccacgtgat ggagcgcgag tcgttcgaga
     6901 acgacgagat cgcggccgtg atgaacgagc acttcgtgcc cgtgaagctc gatcgcgagg
     6961 agcggccgga cctcgacgcg atctacatgg aggcgtgcgt ggcgatgaac ggccacggcg
     7021 gctggccgct gaacgtgttc ctcacgcccg agcaggtgcc gttctacgcg gggacgtact
     7081 tcccgccgga ggagggtcgc gggctgccga gctggcggcg cgtgctggag gccgtcgcgc
     7141 gcgcgtggga caccgagcgc gacaagatcc gcgagggtgg ggagcggacg gcccagcggc
     7201 tggccggcgg cgcccggctc aagccgacgg acgagctgat ggacccgaag cgcctcgacg
     7261 aggcggtcgc cgtgctccgc ggctccttcg atgcgcgcta cggaggcttc ggtcgtgcgc
     7321 ccaagttccc gccctcctcg gcgatcgagt tcctgctgcg ccgcggcgag cgcgagatga
     7381 ccacgcagac gctctgggcg atggcgagcg ggggcatgta cgaccaggtc ggcggcggct
     7441 tcgcgcgcta ctcggtcgac gagcgctggc tggtccccca cttcgagaag atgctctacg
     7501 acaacggtct gctcgcgcgc gcctacctgc acgcgtggca ggcgaccggc gaggagctct
     7561 tccgcgaggt cgcgtgcgag acgctcgact gggcgctgcg cgagatgcgc gcccccgagg
     7621 gcgggttcta ctccgctctg gatgccgact ccgagggcga ggagggcaag ttctacgtct
     7681 ggacgctcga cgagctgcgc tccgtcctgg gcgacgacgc cgacgcggcc atcgattact
     7741 tcggcgccga ccacggcggc aacttcgagg gcacgaacat cctcacccgc gggtccgatc
     7801 cgcccgagcg gctccccgag atcaagagca agctctacgg cgtgcgcaag gagcgcgtct
     7861 ggcccgggct cgacgacaag cggctgacct cctggaacgc gctcatgatc agcgccctgg
     7921 ccgacgcggg cgccgcgctc gagcgacagg actacctcga cgccgccacg gcctgcgccg
     7981 acttcgtgct gcgtgagctg cgtgacaccg agggccggct gctgcgcacg ttcaaggacg
     8041 gtcaggcgaa gctgaacgcg tacctcgagg accacgccta cctcgtcgag gcgttgatct
     8101 cgctgtacga ggcgacgttc gacccgcgct ggttccgtgc cgcgcgcgaa accgcggacg
     8161 ccatgatcga gcgcttcgcc gacgacgaga acggcgggtt cttcgagacc tcgatcgacc
     8221 acgagcggct gctcacccgc cgcaaggacc tcgaggacaa cccgatcccc gccggcaact
     8281 cgagcgcggc gtacgcgctg ctgcggctcg cggcgctcac gggcgagcac gagtacgagc
     8341 agcgagccgt gtcggtgttc cggctgctgc accacgtcgc gccgcagcac ccgcaggcgt
     8401 tcgcgcacct gctccaggcg ctcgacttcc acttcgcctc ggtgaaggag gtcgccctgg
     8461 tgggcgatga tcgcgccccg ctggagcgcg tcgtgcgcag cggctttcgc ccgcacatcg
     8521 tgctggccgg cggcgatccc gacggggtgc cgctgctggc cgagcgcggg cccgtggacg
     8581 gccgcgcagc cgcctacgtg tgcgagaact tcgcgtgcca gcggcccgtt acggagccgg
     8641 ccgagctgga gaaactgttg gcatgagcac gcggctgccc tggaccgagg cgctcgcctc
     8701 gtggcagccg gccgcgatcg tggagcggct gaccgacgac gccgtgatcc gcgtggccgt
     8761 gcacgacgcg ccgatgcagg ggcgcgaggt ggcggagttc ctgttcggcg tgctctcgga
     8821 ggagctggcg gcgccgcggg tcaccgacga gatcgtggag ggcgaccgag cggtcgtgct
     8881 gttcgagacc gagatccgcg ggcaggcggc gcaggggctc aacgtggtcc ggcatgcgcc
     8941 cgacggccgg gtcgccgacc tcaccgtgtt cttccgcccg ctcgagtcac tcgcgacgat
     9001 cgccgaggtc gtcggcgcgc gcatggccga gcgcttcggc ccgccgccgg gttagcgcgc
     9061 cgcgcgtacg tcaacggcga gcacttaggc ccggggcccg aaccgccggg ttctcatcga
     9121 ctggagtctt aggtctctca taggtacata aggctccaat cggtgcgccc catggaggcc
     9181 ggcggcgcgt gcacctcatc gattggagcc ttgctccgcg tggcggtaca aaaggctcca
     9241 atcgatgcgc ggagccgcgg gcgcgacgcg ggcccccggc gcgggagccg cgtcagcggc
     9301 gcggcagcag gcgcacggtc ttgcgcgcgc tcgggtagcc cgccagcgcg gcgctcgcct
     9361 tgcgcaggcc ggcgcgggcg atgcgcacgg tcagctcggc ccggccgtcg gcgttcgtcg
     9421 tggccctccg ccccgcgaag cgcaccgtcg cgcccggcat cgcgcgcccg ccggccgtga
     9481 ccgcgaagcg ccagcgcgtg gacgagcccg ccgccacccg cttcggctcc accgccaggc
     9541 gcaggcgcgg cggcttcgcg gccggcgggc ccgcgggcgg tgggcgatcg gtcgcgtccg
     9601 ccagctccac cagcacgccg ctgaagccca ccacgtacag gttccgcgcc gggaacccgg
     9661 gcgcgccgcc gccccagttc accgcgctgg cgttcatgat cccgctggcc agccggcacg
     9721 ccgtgcggtc ggtgtcgatc cgccagacct cgccgctcgc gttgaccgcg acgaacagcc
     9781 ggtcgcggtc gtcgcgggtc atgccgtcgg ggccgccctg caggtcgggg gcggtcgcct
     9841 cgtagtacgg ctcctccttc gccgggttgc tcaggtcgat ccgcgcgacc gacgccggct
     9901 tgaacgtctg cgccgcgtac agatagcgcc cggccgtgtc gatcacgagg ccgttcggcg
     9961 tctgcacctt cgaccagtcg tcctccacct tgccgtcctt cacgcggtcg atcccgcccg
    10021 cgaagtcgtt cgaggcgtag aacgcgccgt ccggcccgcg cacgattccg ttggccatcc
    10081 ccatgccgct cgcgagcggc gcggtctcgc cggtctccgg gttcacgtgc agaagcccgc
    10141 ccgcggtccc gtcggccacc gcgttctggt tgccgccgtt gaagccgagc acgaggctgc
    10201 cgtcggtgtc ccaggcgagg ccgccggtcc cgttgatgcc ctgtacgagc tgcttcggct
    10261 ccgcaccggg cgcgtcgagc ctgaacaggc gcccgccgtt ctggatgtcg ctgtagaaga
    10321 tccgaccgcg ggcgtcggag atgatcgact cgagctggcc ctgcccggtg agcagcgtcc
    10381 tcttctcggg cagcggcgag cagtcgggga gggcgtgggc gggcgacgcc agtgccagcg
    10441 tcgccagaac cgccgcgacg agaagacgga cgctcacgcc aggccgctca gcaccagctc
    10501 gtcgtgcccc tgcgccttga cgttgcgcag cacgcgctcg atccgcccgt ccgagccgat
    10561 cacgaacgtc gagcgctcga tccccatgcg ctccttcatg tacccgcgcg cgggcttgtg
    10621 gacccacgtc ccgtacgcct ccgccacggc gtggtcctcg tcggagagca acgtgaacgg
    10681 cagcccgtac ttgtcggcga acttccgcag cgccggcacg gggtcgggcg agacgcccag
    10741 caccaccgcc ccggccccct cgaactcgcc ctggcggtcg cggatcgcac acgcctgcgc
    10801 ggtacacccc ggcgtgtccg ccttggggta gaagtacagg acgaccgtct tcccgcgtag
    10861 cgacgagagc cggaccgcct ccccgtcctg gtcgcgcagc tcgaagtccg gagcctcagc
    10921 cccctcctcg acgctcacag gaccgccagg tagcgatcga gctcccaggc cgagacctgc
    10981 acgcggtagt cgtcccactc cttgcgcttc agctcgacgt agcgcgtgaa catgtgctcg
    11041 ccgagcgtgc gcagcacgag ctcggactcc gcggtcagct cgatcgcctc gccgagcgtc
    11101 tcgggaagct gctcgatccc gcgccgcttg cggtcgtcgg ccgacaggtg gtagaggttc
    11161 tgctccatcg gctcgggcag ctcgtagccc ttctcgatcc cctcgagacc ggcctggagc
    11221 aacgtcgcga acgtcaggta tgggttgcag gccggatccg ggcagcgcag ctccatccgc
    11281 gtggcctgct ccttgcccgg gtggtagagc ggcacccgga cgagcgcgga gcggttgcgc
    11341 cgcgaccagg ccacgtacac aggcgcctcg tagcccggca cgagccgctt gtacgagttc
    11401 acccactggg cgaagatcga cgagatctcg cgcgcgtgac ggagctgccc ggcgatgaag
    11461 cacttgccgg tggccgacag gtagtactgg tcgtcgccgt cgtagaagga gttgcggccc
    11521 tccttgaaga gcgactggtg cgtgtgcatg cccgagccgt tctcgccgaa cagtggcttc
    11581 ggcatgaagg tcgcgtgcca gccgtacttc atcgcgtact ccttgaccgt gatgcggtac
    11641 gtcatgcagt cgtcggccat cttcagcgcc ggcgcgtagc gcatgtcgat ctcgtgctgc
    11701 gaggggccga cctcgtggtg ggtgtactcc acgtggatgc cgagctgctc gagcgcgagc
    11761 acggtgtcgc ggcggacgtc ggagccggcg tcgagcgtgg tgaggtcgaa gtagccgccc
    11821 tcgtcgagca cctcgggcac gccgctctcc ggcttggagt tgcggaagta gaagaactcg
    11881 agctcgggcc cgacgttgaa cgcgtcgaag cccatcgcct cggcacgctg gagcgcgcgg
    11941 cgcaggacgt ggcgcgggtc gccctcgtac ggctggcggt cgggcgtgac cacgtcgcag
    12001 aacatgcgcc ccaccccgcc gccgccctcg gacggccgcc acgggagcac ggcgaacgtg
    12061 gacgcgtcgg gcatcgcgat catgtccgac tcctcgatcg cgttgaagcc ggtgatcgag
    12121 gagccgtcga accccatgcc gccctcgaag gcgtcgtcga gctcctcggc gttgatcgag
    12181 aagctcttga gctgacccag gatgtcggtg aaccagagcc ggatgaagcg gatcttccgg
    12241 tcggctacca gtgccttgac gtcgtcggct gtctgcggcg tgtcggccat gaggacgact
    12301 cctaggtgct cgggatcggc gggctacgcc gcagactaac gagctagcgc aggtggcttc
    12361 gggcccacgc ctggagcgtg cgcagagccg gctcgagcgc ctgccccttg gccgtcagcg
    12421 agtactccgt gcgcaccggc gagccgtcga tcacgcggcg cccgacgagt ccctccgact
    12481 ccagctcctt gagccgctcg gacaggagcc gatcggagat gtccggcacg gcgcgccgga
    12541 gctcggagaa gtggagcggg ccgtcgagga ggacggcgag gatggcgccg gaccagcgct
    12601 tgccgatcag ctcgacggcg cggtggtaat aggtgcagca cgtccggcgc tcgactgtca
    12661 cgcgaaccgc catcgggcgc atcgtagctc cccagcgggt cgcggccgcg cgggtcaggc
    12721 cgcgcggagc acgcgcccaa gctcggcgga gccgtcgagc gggacgttcc cgccgcgggt
    12781 gatcagcgca tggaagaggc cgagcgacac gtgcgcggtg gcctcgatga gctgctcgtc
    12841 ctcccagccg gcctcgcgtg cctcctcgac gaggtgcagc ggcacggcgt ccggagcctc
    12901 cagcaggccc ttcacgaacg cgagcagcgc cgcctcgcgc cggtcgatcg agcggaactc
    12961 gcgggcgagc ccgatctcgt cgaggccgag acccgcgtcc cgggcgctgc gcatgagcat
    13021 cgagagcgcg tagtcgctgc cctcgtgctg ggcgacgccg agcgcgacgc gctgctggga
    13081 cttgagcggc agcgatccct gacgcaactc cgagcggaag cgggcgtagg cgcgcagcac
    13141 ggcgggggcg ccggcgagca cgccgagcat gttcgagacc tggccgccgg tcgagagggc
    13201 gcccttgagg atgggcacgc tgcgctccgg ggcgctcatc tcgtcgtgga tgtggaacct
    13261 gctctccttc gccttcttcg ccacggtcgg cctcttgtca gttgcttacg agaagtaagt
    13321 ataggttgcg gtgtctgccc gagagccggc gcccgcctca cttggaatac gcgcgtcgtc
    13381 ccgtcacgga tgtgtacggg cgtgctgtga acgctcccta aaccgggcgc gcggaagtgt
    13441 gcgatcgggc cgctttgggc gggctagcgg cccggttggg gggtgcgagc gggccgctta
    13501 gagcggcgcg gcgtcggcgg tcgcggtggc gaccccgacg aggaagatgc ccatgaagaa
    13561 cgcccacatg gcgatccggt ccggtcgcgg gcccacgcgg ctcgcggcgt cagggcgacg
    13621 gcgcggcgcg gcgggcgcgg cgcgctggcc ggtgatcgtg accgtgcggc ggccggtgtc
    13681 cgggtcgcgc cgcggggcct cctcgccggt gagggtgacg acaaggctca gcgcgggacg
    13741 tacggcgtcg cgctcgcccg cccggcgtcg cggacgcgcg gcatcgcgcg cgctcgcacg
    13801 ccggcgcgcg tgctcggcct cgcccacggg ggcggtacgc ctatgtacgt cgtcccgcca
    13861 tgcgcgggct gctgcacgat ctgccatgga acctctttcg cgcgcctacg gggttagctg
    13921 acgggctcgc gctgaaagag ctgcgctacg ccgcttcctg cggcgattcg cccctcggcc
    13981 ttctcggcca ctggttcccc cgtgccccgc cactggcggc gggacctcgg ctatcgggtg
    14041 ctgatgctac ccactttcgt ctccgaacga acgtgaatga gagcggacga ggcggacgga
    14101 ctactcgcgg cccgcgacct cggtgaggta cgcgccgacg gcctccgcgt tctcgcccgc
    14161 gagcagccgc ggcggcatcc gcccctggcc ggtgccgccg atctcgatcg ccttcaggat
    14221 ccgctgcttg gtcacctgcc cgatctggtc gaggttcggg ccggtgacgc cgcgggcatt
    14281 ggccgcggcg aggctgtggc agctcgcgca ggtctggcgg aagaggtcgc ggccctgggc
    14341 cacctgcgcg gccggcttct tgtccttcag gtcgcccacg ccgccctccg cccgatcgcg
    14401 gccggcgatc acgagcaccg gcaccgcgat cccgatggcc acgtagatca ccgggatgac
    14461 gaagcgaaac gcccgccggc cgccggtcag gtacgcctcg cgggcgcggg tcggcccgcc
    14521 ggagaacgcg acgaagagca ccccgacccc gagtgcgagg aaggctaaga cgaaggcagc
    14581 ggccgccatg cgggcgggga tcctatctcg cgtgtcggag gagcggcggg gcggccctca
    14641 ggccgcggcc tcgtcgcccg agagctcgcc ctcgtcgccg gacagcgcgg cctcgcggcg
    14701 agcgcgcttc cagctcccga atgcggcgaa gaccgaggtc aggtcgggca ggtcgtcgtc
    14761 ctcggcggcg ctctgctggt agtcgaccat cagcagccgc tcgggcggag agccgagccg
    14821 ctcggccacg gtgcggatcc cctcgatcac ctcgtcgggc gagacgacct ccgcgctgtc
    14881 gcggccgcgc tgggcctcgc gggcgaacac ccccgcggcg gcgccacggg cgcccggggc
    14941 gccgtcaccc gcgtcacgcg cgcggccggc gccgatcagc aggcggcagt gagggcagcg
    15001 caccggctgc ggtggccagg ccggtagggc gtccgcctcc accagcagga ggcagtgcgg
    15061 gcagtgcgcg tcggtccgct ctgaggtcgt tgccatcacc ctcgttgttg cgcaacggcc
    15121 gtgtgggtaa accccgtgcc gttgggcggg cgcgccagcg cgccgaaagg ggctctgcgc
    15181 ctagaccgcg agcgcctgga gctcgcgctc ctcggcgagc ttgcgcaggg cgcggcgctc
    15241 gagcttgcgc acgccctcgg gcgagaggcc catccgcttg gccacctcac gcagcgtctt
    15301 cggctcgcgg ccgtcgagcc cgaagcgcat ctcgatcacg ctgcgggccg tgtcgtcgag
    15361 ccgctcgagc gcgcggcgga tcgactcctc ctcgagctcg accgcgacct cctcgagcgg
    15421 gctgggcgca tccgaaggga gcagggaccc gagctcggcc tccccctccc ccccgaccgg
    15481 cgtctccagg ctcaccggca cccgctccgc cgagcgcagc tcctcgatcc gcgccgggtc
    15541 ggtgccgagc gcctcggcca gctcgacctc ggtcggctcg cgcccgagcg cggctgtgag
    15601 cttgcgctcg gtcgtgcgca gcttggtcag ctcctggccg atgtggaccg ggattcggat
    15661 cgtccgcgac tggtgctgaa cgccgcgctg catcgcctgg cggatccacc aggtggcgta
    15721 ggtggagaac ttgaagccgc gccggtggtc gaacttctcg accgctcgga tcagcccgag
    15781 gattccctcc tggatcaggt cgaggaacgg gagaccgagg ccgcggtagc gcttcgcgtt
    15841 cgccaccacg agccgcaggt tcgcctcgat catgcggtcc ttggccgcct gatcgccgcg
    15901 ctcgatgcgc ttggccagct cgatctcctc ctgcgcggtg agcagcggcc gctgcgcgat
    15961 gtcgcggagg aagagctgga gcgagtcgct cgtggcctcg gccacggcgt tgtgcgtgaa
    16021 gcgcgcctcg ttggcgccgc tgcgctcgtt gtggtcgagc acctcgatcc cgcggcgctc
    16081 cgcctcgcgg tagagcgcct cgatccggtc gtcgtcgacg tccaggccct cgaccgccgc
    16141 ggccacgtcg gagaactcga gatagccgtt ctcctcggcc cggcggagca ggtcgtcgat
    16201 cctcccttcg agtccctcct cgctcatggc tttcagcata cgcccgctca cccgagagcg
    16261 attcgcctcc ggggaccgca tgacggcgcc tccgcggcgg acggccggta ccatcgccgc
    16321 cgtgaggggt tggttcgtcc tgggggcgct cgcggtactg ctcgcgctcc ctgcgagcgc
    16381 cgcggcgcgc gtcgtgcgcg cgggcgacat cctgcccccg ggccagagcg gccacgtgac
    16441 gctggcggga gtgccgagcg gcaccgggtc gccgcacctc accgaccagc tcgagcgctt
    16501 cctggccttt gagttcaagc cgcacgggtt cgacaacccc gcgcagtcga tcgagacgca
    16561 gcggatcggc gtgacgctcg agcgcgacga ctggggcgtg ccgacgatcc gcggccagaa
    16621 cgacgacgac atctggttcg gcgccggcta cgcggtcgcg caggaccggc tgttccagat
    16681 ggagctgttc cggcgcgcga cccagggaca cctcgccgag atcgtcggcc gcagctacct
    16741 cgaggacgac atcaagatcc ggcgcgactt ctatacggga cccgagctcg acgcccaggt
    16801 cgcgagcctc cccgacaact acgaggcgcg ctatcgcagc tacgcggcgg gggtcaacgc
    16861 gtggatcgcg gagacccgcg ccgacccgaa caagctccct gccgagttcg cgctggtgaa
    16921 cgccgcgccg ccggcggact ggacggtgcg cgactcggcc gcgatcagcg tctacctcgc
    16981 gcgcacggtg ccgagcgacg acggcgagga gatgggcaac tggaaggcgc tcaccacctt
    17041 cgggtcgaag ctcttcgaca gggtcctgcc gatccggacg aagggccagg tgccgaccgt
    17101 gccgcccagt gagggctcgt tccccgcgca gcccggccgc aaggcgaagg acgaggccgc
    17161 gggccgcaag cgctcgaagg cgttcctcaa gagcgtgccg atgccgaagg aggaggacgt
    17221 cggcggcacg cacgccgccg cgcgcgagct cggcgcgctg ccgatgctcg gtcagctcgg
    17281 ccagcgcggg tcgaacatgt gggcggtccg caacaagaac gtcggcgcga cgctgttcaa
    17341 cggtccgcag ctcggcttcc agatccccga gctgttcgtc gaatacgagg tgcaccggcc
    17401 cggctacgac gcgcgtggcg tgaccgcgcc cggcgtgccg ctcgtcgcca tcggccacaa
    17461 cggacaggtc gcgtggggct tcacgagcgg actgaccgac gaggacgacc tctacgtcga
    17521 gaagctcacc ggtcccgaga agtacatgtt cgagggcaag gagcggcaga tggaatgccg
    17581 cgacgagacc ttctcctacc ggtcgcctcc ctcggacgcg accagcatcc tcgacctcga
    17641 gccgtccccg ccggagtccg gctcggagac gcgccgcctg tgccgcacga tccacggacc
    17701 ggtgcagggc cgcgccgagg gcaccgcctt cgcgcggcgc tacgcgatct ggggacgcga
    17761 gttcgacacg ctcggcggac tcgccggcgt gagcgagtcg aagaacgtgg ccgaggtcga
    17821 cagggcgatg gcgaaggtca cctggaacga gaacctgatg gccgccgaca gccagggcaa
    17881 catcggctac tggcatcccg ggctgctgcc gatcaagccg cgcgggtatg acgagcgcct
    17941 gccgttcccc ggcaccggcg aggccgagtg gagggggttc ctgacgcccg cgcagcggcc
    18001 gcactcgatc aacccgaagc agggctggct cgtcaactgg aacaacatgc cgtcggccgg
    18061 ctggactcag ggcgacatcc ccgcgcgcga gcgcgggctc ggcccgtacc accgcgccca
    18121 cctgctctcg aaggtcgtcg cgcgtgcgca cgccaacggc ggcggctacg agcagacgcg
    18181 tgcggtggac cgggtcgtcg gcaccaccgc gcagcagcgc ccggtcgccg ccgggccgct
    18241 caactccgcg ctacggggcg cgaccggcaa cgcgaagacc gtgctcgaga cgatcaagcg
    18301 ctggaacggc tcctaccacg tgaccgacgc gaacgggacg gtcgacccgg gcgtggccgc
    18361 ctggcgcgag ttcgcgaacg ccgcgctgaa gatcggcttc gggaagctct gggacagcaa
    18421 ggatctgcgg ctgttcgaga cgagcgcggg cagctcgcac cggcaggagc tctcgaacgt
    18481 gcaggtctac gccctgcgga cgatcggcgc gaagggctgg cgccaggcgg ccaagctcgc
    18541 gttcccggcg ctggctgcgc ggtttggcag cgaggatccg gcgaagtggc gcgacaagcg
    18601 gctgatgtac aacccgagcg cccagggcgc cgcgagcttc gacccgatcc ccttcctcga
    18661 ccgcggcacc tggcagcagg tggtcgagct tggcccctag ggcctcctct cggcgcgacc
    18721 tcctcacccc cgcggccagc aaggcgccgg tccggcggcg cgcggacgcc gtcgtggtcg
    18781 gcgccggctt cgccggcctg accgccgcgc gcgaactcgt gcgcgcgggg cgctcggtga
    18841 tcgtgctcga ggcccgcaac cgggtcggcg ggcgcacgct gaacgcgccg atcggcggca
    18901 acgaggtgat cgaggtcggc ggccagtggg tcgggccgac gcaggaccgc ctgctcgcgc
    18961 tcggcgaggc gatgggcgtg ccgacctaca agacctacaa cgagggcgac aacctctact
    19021 accgcggcgg ctcgctccag cgcttcagct cgaccgggcc gctcggggcc gtgccgcccg
    19081 atcccgaggg tgcgccgcag gcggcggcct tcatccagca gatcaacgac atggccgcgc
    19141 gcgtcccgcg cgacaggccg tgggaagccg cgagcgccgt cgagtgggac tcgcagaccg
    19201 tggagacctt caagcaggcg aacgcgccga ccgagggcgg gcgcttcctc ttcgacgtgg
    19261 gcgtggaggc cgtgttcgcc gccgagccgc gcgacatctc gctgctctgg ctcctcttct
    19321 acacggcggc cgccgggaac gagacgaccc cgggcgactt caaccggctg ctcaacaccg
    19381 ccggcggcgc gcaggagagc cgctacgtgg gcgggtcgca gcgggtggcg ctgcgggtgg
    19441 ccaagcagct cggcaagaaa cggatcgtgc tcggctcgcc ggtgcggcgg gtcaagcagg
    19501 gcggcaagag cgtgaccgtg gagtccgacc gcgcggtggt gaacgccaag cgcgtgatcg
    19561 tcgccgtccc ccccacgctc tgccagcgga tcgagttcgc gccggggctc ccggccgcgc
    19621 gcgaccggct cacgcagaac tggccgatgg gctcggtggt caagtgcgag gccgtgtacg
    19681 acaagccgtt ctggcgcgag gaggggctga ccggccaggt ggtgagcgac gcgagcccgg
    19741 tgaagatcac gttcgacaac acgcccgcga gcggcacgcc cggcgtgctc ctcgggttca
    19801 tcgagggccg cgagtcgcgc gtgtggggcg agaagtccgt cggcgagcgc cgggcggccg
    19861 tgctgcgcaa cttcgccacc tatttcggcg agcgcgcctt gaagccggcg cgctacgtcg
    19921 agatggcgtg gcagcacgag acctggagcg gcggctgcta cgcggggaat ttgacgccgg
    19981 gcacgctgct cgacttcggc ccggcgatcc gggagccggt gggccggctg cactgggccg
    20041 ggaccgagac ctcgacgctg tgggccggct acatcgacgg cgcggtgcgc tcgggggagc
    20101 gcgcggcggc ggaggttctg gcgaggcttt gaacagttga tgaccctatg gggtcattaa
    20161 ctgttcaaag aagtgaggta ggcgtcggtc cgccagcgct ggtcgaacgc cgcgacgccg
    20221 tgctccgcga gcaccgcggc cacgcccgcc agcagctcgg tgcggtccgg gtctgggagc
    20281 gctccgacga acgaccagct gctcaccagg gcgacgtagt cctcggcggt gatgcgctgc
    20341 gtgtgctcga agctgcggag ggtcagcggc tcgaagagct cccagcccgg cccggtgcgc
    20401 caggcggcgg ggtcgtcgcg cgggtcgttg cccgaggcgg acggagtgcg gcgaccctcc
    20461 gggtggtagt agacgtcgcg aaccagctcg accacctcgg cgcgccaggg ctcctcgtcg
    20521 tccgcggtca cgcccacgtt ccacagcacc gcgagcccgc cgcccgggcg cagcacgcgc
    20581 gcgatctcgg ccagcgcacg cggggcgtcg aaccagtgga acgcctcggc gacgaacacg
    20641 gcgtcggcga aggcgtccga gagtgggatc gactcggcgg tgcccggaag ggtctcggct
    20701 ccactgatga gcgcgcgcat ctcgtccagc ggctcgatcg cggtcacgga ggctcccgcg
    20761 gcgagcagct cgcgggtcag gcgccccgtg ccggcggcga ggtccacgac gcgggcggcc
    20821 tgcggggcga gcgaccagtg gtcgaacgcg gcgtgaacgg cggcgggcgg ccaccccggc
    20881 cgcgcgcggt cgtaggcgcg ggcggaacgg ccgaagccgg agctcgcgac gggattgacg
    20941 gcggccgaag ccgcggtggt cgagggcgcc gcgatcgggg gcccggcgcc ggccggtggc
    21001 ttcgcctcgc cgctcaggcg cccgcggcgg ccagctcggg ctcgcccgcc gcgccgccga
    21061 gcccgctcag gtagcggtcc gcggagcgcg ccgcctggcg accctcggag atcgcccaca
    21121 cgaccagcga ctggccgcgg cgtgcgtcgc cgcaggcgaa cacgcccggc accgaggtgg
    21181 cgaagtcgtc gtccgacacg ttgccgcgcc cgtcgcgctc cacgccgagc tggtcgacca
    21241 gcccctcgtg ctgcgggtgc aggaagccca tcgcgagcag gacgaggtcg gcctggatcg
    21301 tgaactccgt gccgtcgacc ttgtcgaacc ccggcgggcc ggccacctcg tggccgtgca
    21361 ggtgagtcac gcgtccgtcc tcgcccgtga ggtgcgtggt catcacgctg tagcggcgct
    21421 cgccgccctc ctcgtgggcg ccggaggtgc gcaggaccag cggccactgc ggccacgggg
    21481 tgcggtcgtc ggggcgctct ggcggcggct cgggcagcag ctcgaattgc gtgacgctgg
    21541 ccgggcgctc gcggtgcgaa ttgccgaggc agtcggcgcc tgtgtcgccg ccgccgatga
    21601 tcaccacgtg cttgccggcg gcgctgatcg gcctctccgg gggcgggtcg ccggccacga
    21661 agcggttgcg ctgctcgagg tactccatcg cgaagtgcac gccgtcgagc tcgcggccgg
    21721 ggaccggcag atcgcgcggg atggtcgagc cgatggcgag cacgacagcg tcgaactgct
    21781 cgcgcagctc gtcggcggtg atgtcgacgc ccacgttcac gcccgtgcgg atctcgaccc
    21841 cctcggcctc catcaggtcc gcgcggcgct gtacgaagcg cttctcgagc ttgaagtccg
    21901 gcacgccgta gcgcatgagg ccgccgatgc ggtcgttgcg ctcgaacagc gtgacgctgt
    21961 ggccggcctt gttgagctgc gccgcggcgg ccatgcccgc cgggcccgac cccacgaccg
    22021 cgaccgtgcg gccagtccgg aacggcggcg gctcgggctt gatccagccc tcctcgaacg
    22081 cgcggtcggc gatcgcctgc tcgatctgct tgatcgtgac cgagtcgtcg ttgatgtcga
    22141 gcgtgcacgc ggcctcgcac ggcgccgggc agatccggcc ggtgaactcg gggaagttgt
    22201 tggtggcgtg gagggcctcg atcgcctcct tccagcggtc ccggtagacg aggtcgttcc
    22261 agtccgggat caggttgccc agcgggcagc ccgagtggca gaacgggatg ccgcagtcca
    22321 tgcagcgggc accctgctcg cgcagcccgg cctgcgggag cagcaccacg tactccttga
    22381 agtcctcgac ccgctcggtc ttcggccgct tgttcggctc gacccggtgg atcttgagga
    22441 agccgccgag ctcacccacg gcgggtggcc tccctggggg tggcgctacc cgccatgcgg
    22501 gaggacctcc tcctccgcgg cggcggcggc ctggtccgcg gcggcctcgc cggcccccgc
    22561 ggcctcggcg cggcgctcgg cacgcgcacc cgcgtccgct gcgtcctcgg tctggccggc
    22621 ctcgccggtc tcggtggtct cctggtcgcc gtcggccacc gggtacggcg agccctggcc
    22681 cacccggacc ggacccgctc cgtcgccatc gccgtgctcg gccacgatcg ccggctcgcc
    22741 ggcctcgatc cgctggcgct cctggcgctc gcggagcact cgcttgtaat ccatcggcat
    22801 caccttcacc caggcggagg tcagggagtc ccagtcggcc agcacgcgct cggccacggt
    22861 ggagcccgtc cgctcgcggt gctcctcgac cagggcgcgg atctcggcga agtcgtcctc
    22921 gtcgggatgc tcgagctcga ccatctcggt gttgcacttg gacgcgaact cgtagtcgcg
    22981 gtcgagcacg tacgcgaccc cgccgctcat gcccgcggcg aagttgcggc ccgtcggccc
    23041 gaggatcacc acgcggccgc cggtcatgta ctcgcagccg tggtcgccga cgccctcgac
    23101 caccgcgtgg gcgcccgagt tgcgcacgca gaaccgctcg cccgcgagcc cgcggaagaa
    23161 cgcgcgcccg gcggtggcgc cgtagagcac ggtgttgccg atcacgacgt tgtcctcggc
    23221 cgcgaacggc acgtccgcgg gcgggcggac cgtgagcacg ccgccggaga ggcccttgcc
    23281 ggtgtagtcg ttggcgtcgc cggtgagggt gagcgttacg ccgggcgcga gccagccgcc
    23341 gaagctctgg ccgccgctgc cctctagctg cacgtcgatc gtgccctcgg ggaggccgtc
    23401 gatgccgtag cggcgcgcga tctcgccgga gatgtagccg cccacgcagc ggtgttggtt
    23461 ctcggcggac accgagaagc tgaccttctc gccgcgctcg agggccggct cggcgagccg
    23521 catcagctcg tggtcgaggt ggtcgtcgag cacccgatcc tgcttgcgga cgcagcggcg
    23581 cggcacgtcg gccggcgcct gcggcggcgc gagcagcccg gagaggtcca ccccgcgggc
    23641 cttccagtgg tcgatggcgg cgtcgggctc gagcaggtcc gtgcggccga ccaggtcctc
    23701 gaacttggcg acgccgagct gcgccatgta cgcgcgcacc tcctcggcga tgaagaagaa
    23761 gaagttcacg acgtgctcgg gctggccctg gaagcgcttg cgcagctccg ggtcctgcgt
    23821 ggcgatcccg accgggcacg tgttcaggtg acacgcgcgc atgtagatgc agcccatcgt
    23881 gatcagcggt gccgtggaga aaccgaactc ctcggcgccg aggagcgcgc cgatgatcac
    23941 gtcgcggccc gtcttcatct ggccatcggt ctgcacgacg atccggtcgc gcagcttgtt
    24001 catcacgagc gtctgctgag tctcggccag gccgatctcc cacgggacgc cggccgactg
    24061 gatcgacgag accggcgagg cgccggtgcc gccgtcgtgg cccgcgatca ccacgtggtc
    24121 ggagttcgcc ttcgcgacgc cggcggccac cgtgccgacc ccaacctccg agacgagctt
    24181 cacactgatg cgcgccgtcg ggttcgcgca gcgcaggtcg aagatgagct gcttgaggtc
    24241 ctcgatcgag tagatgtcgt gatgcggcgg aggcgagatc aggccgacgc ccggcgtcga
    24301 gtgccggatc ttcgcgatgt actcgtcgac cttgtggccg gggagctggc cgccctcgcc
    24361 gggcttcgcc ccctgggcga ccttgatctg gagctcgtcc gcgttgacga ggtagtgggc
    24421 ggtcacgccg aagcggcccg aggcgacctg cttgatcgcc gagcggcgtg agtcgccgtt
    24481 ggcgtcgggc gtgtagcggc gcgggtcctc gccaccctcg ccggtgttcg acttcccgcc
    24541 gaggcggttc atcgcgatcg cgagtgtctc gtgcgcctcg gccgacagcg atccgagcga
    24601 catcgcgccg gtggcgaagc gcttcacgat gtccttcgcc gactcgacct cctcgatgtc
    24661 gatcggctcg ccggggtcgc gcacgtcgag cagcccgcgc agcgtcgcgc gccgcttcga
    24721 ctgctcgttg atcatcttcg tgaactcctc gtaggagtcc tggccgccgt ggcgcaccgc
    24781 gtgctggatc ttcgcgaccg tgtcggggtt ccactggtgg aactcgccgt cgcggcgcca
    24841 catgtgcacg ccgccgacgg gcagacggtg gctcgagcgc gggtaggcgc gccggtggcg
    24901 ctccatcgtc tcgcgcgcga gcacgtcgat gccgatcccg ccgatgcgcg aggtcgtgcc
    24961 cgtgaagtac ttgtcgatca ggccgcgctc gagcccgacg gcctcgaaga tctgcgcgcc
    25021 cgagtagctg cgcacggtgg agatccccat cttcgagatc gtcttgagga tcgccttctc
    25081 catcgccttg acgaagtgct tctcggcgtc ggcggcggac tcgacctttg ggaggagccc
    25141 gtcgtcgaac atgtcgtgca gggtctcgaa ggcgaggtac gggttgatcg ccgacgcgcc
    25201 gtagccgagc aggcagcaga cgtggtggac ctcgcgcggc tcgccggact cgaccacgag
    25261 cccggtctgc aggcgcgtgc cctcgcggac caggtggtgg tgcacgcccg agaccgccag
    25321 gagcgcgggg atcgggacgc ggtcctcgcc cgcggcgcgg tcggagagga tgaggatgtt
    25381 gtcgccctgc gcgaggcgct cggaggcctc ctcgcagagg cgctccatcg cacgcgccat
    25441 gccctcgggg ccgtcggccg ggggccaggt ggcgtcgagc gtgtgcgcgg cgaacacgtc
    25501 gtggtcgacc tggcgcagct tctcgagctc gccgctcgag aggatcggct ggccgatgat
    25561 gagctggtgg gcgtgctcgg gcgtctcggt gaagaggttc gactgcggac cgaccgcggt
    25621 ctcgaggctc atcaccacgc gctcgcggat cgggtcgatc gccgggttgg tgacctgggc
    25681 gaagagctgc ttgaagtacg agtagagcgg cgggctcttg tcggagagga cggccaacgc
    25741 gaagtcgttg cccatcgacc cgatcggctc ctcggcgctc gccccgccca tgcgcgcgag
    25801 cgtgatgcgc aggtcctcct gcgtgtagcc gaacatgagc tggcgggtgc ggaccgggtc
    25861 ggccgggatc tcgcgcggcg ccaccgcggg gaggtcgtcg aggtggacgg tgcagtcctt
    25921 gaaccactgc ccgtacggct tgcgcgccgc gatctcgtgc ttgagctcgg cgtcctcgat
    25981 cacgcggccc tgctcgacgt cgacgaagaa cagcttgccg ggctggaggc ggcccttgcg
    26041 gaggatcgtg ctgggctcgg cctcgagcac gccggtctcc gaggcgagca ccacgaagtc
    26101 gtcgctcgtg acctgccagc ggc
//
LOCUS       DASVPL010000274        28113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_72629, whole genome shotgun sequence.
ACCESSION   DASVPL010000274 DASVPL010000000
VERSION     DASVPL010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 28113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28113
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72629"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VF517_15250"
     CDS             <1..602
                     /locus_tag="VF517_15250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8054344.1"
                     /translation="ERIAPGAGTLHVVPLGIDLDAIKRTTPYDPDGPVVAVGRLVEHK
                     GFADLAAVAGGVGRGVVVAGEGPQRKELEHLAAGAMTFRGALSPAAARDLIETASVLV
                     APSVIATDGSRDGIPMVIKEALALETPVVASDAVGNPEVVKPEHGALHRAGDRQDLAE
                     KIRLTAGREALGKAGRAFAERHADLRTQTARLAELFRRP"
     gene            complement(589..1878)
                     /locus_tag="VF517_15255"
     CDS             complement(589..1878)
                     /locus_tag="VF517_15255"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8054345.1"
                     /translation="MIGSDTGRAAGLAAAVIVGNVMALAFTVVFARILGGAGYGSLAA
                     LLSSFIILMVPGQALQTTVAREISAELAAGDPAAGAGVRRWLGRLLVAGVVIVVVSVL
                     ARDVLAAVIGVHDLPWAAAATLPTGLLWLVLSVERGALLGFQEYKVVGVSLALEQFMR
                     LVLGLLLVAGVSGGEAEITAAFLGTPLALVVVGLCLLIPLRRNLPNARHAHEHPLREL
                     VRRAAVPVVALGLVAWLQDGNVIVVKHLASDDAAGDYAAAAVAAKAIMWVAVGLSLYL
                     VPEAARRTHLGERASGVLARTLGLILAIAAPMVIVYAVAGEIVLRVAFKLTGAAEALP
                     WLGLAMSLLALTYLAVQYQLALHRAAFIGILAAAAAVQPAAMIAIGGEDLRAIALGLL
                     GVNMLLAAGSVALAFRAVGQVDEIPDESGTSQALTGV"
     gene            1981..3081
                     /locus_tag="VF517_15260"
     CDS             1981..3081
                     /locus_tag="VF517_15260"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8054346.1"
                     /translation="MQERALAERLITYDTSELDGIQAAAGFVKGWLESNDVEIKDGTH
                     NDLPVLAATVGPEEGPTVVLHGHIDVVPGAPEQFEPRIDGDRLIGRGAYDMKGGLAGM
                     MCAVRDLKDQTDVRVHFVCVSDEESDEPFSRGSDYVVEKGYIGDFAITGEPTDLRIGV
                     QAKGVLALRIEVTGVSAHGSTPWLGDNAVLKAVDVFRQIQSMPFAREASDLFDRPSIS
                     LGRIAGGDRINTVPGACTVDVDIRYLPGQDPRAILAGIRALPDIEVSVVLQRDPVNVE
                     RDNPFVVALADTLSEWLPADRVSIGRDGASDAISFIDAGVPAVEFGPVGGGHHGPDEW
                     VSIESLERYSRALVTFISELPASAGKGHLRIA"
     gene            3074..4504
                     /locus_tag="VF517_15265"
     CDS             3074..4504
                     /locus_tag="VF517_15265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX8054347.1"
                     /translation="MPDPKRGIFGRYLIGALLIVLASAGATGVAAFEELDSVVNAFRR
                     GQTLELDDELQKADAGKPQTIMLIGSDTRGKGSRDYTGERGLSDTMMLVRLDPSKNQI
                     AVLSLPRDLKVRIPGHGVDKLNAAYALGGAKLTLKTVKDFTGLGVNHVVNVDFRGFRK
                     AVNALGCVWVDVDRRYFNDNSGGDQYATIDINPGYQKICDQDALDYVRYRHTDNDLVR
                     AARQQDFLRSLKSQIGVNRIVRDRKELISIFGKYTQSDIGSRKTVLRLLKLVVASAGL
                     PIHQIHFKANIGASYVTATSEQTHELADEFLGVEGPAPDPTKPGKKRKRKKQADLRLT
                     DSSVMGKQMALQLVNEGLRGIEIAYPRKLPAGASFQAPPRSYTIRAGQRKKKHRSFRM
                     VISAGRIGEYYGLQGTTWKNPPILSGPHENREVGRQKVQVYYDGPRVRLVAWRSGGAT
                     YWLSNSLLQRMNLDEMLAVVRSTRPL"
     gene            4518..7220
                     /locus_tag="VF517_15270"
     CDS             4518..7220
                     /locus_tag="VF517_15270"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8054348.1"
                     /translation="MAEREPIGVIGAGWVGLVTAACFAELGHEVHCRDIVQEKVDTLR
                     RGEVPIYEPGLPELIEKNGERINFTTDMGEVLSGANLLFCCVDTPPTYSGDADLSRVE
                     RVVEELGDSRDHAIVMKSTVPVGTGRSIRRRREDLGYVSNPEFLKEGSAVADFMQPDR
                     VVVGAGDESQGFAHRVAALYEPLDSPIVRTDVASAEMVKLASNAFLATKISFINEIAN
                     VSEELGADVTEVARGMGLDERIGTQFLRAGIGYGGSCLIGEETVMIRRRGMCALIALE
                     ELFEQIADEQDLDHLDPEVLHPDGLEVLSWREGRPRPEFLPVPVLTRRHYEGDVVDVT
                     TKMGRRVTTTADHPFVVADTGGAEVRTKLASELSTEEDWLPIAQGAPITAVEDVQLEP
                     LEGAVMAELQPADVIVRLAEPELAALAAAPRERWVSGLDHPRGVTQRRSDILRRGALR
                     LHEANRLGASTRGAAALTARNGTAIPFGLRLDAAFWRVVGLYIAEGHMSQGHRIHWSF
                     HPTDETDLVEEVHDFWEARGVKASVFRHPTATEVRVSSRLLAGWLEAFGLGRNAYEQR
                     IPDAAWSQDEATKRALVAGMWRGDGSWSFVRGGPSVVLEYGTISRELADGLLRLLGDL
                     GVVARLKVGRTKKSTADTYWLVVSGAQQVERLLDCVPAADRGQIRASLARQAKRIAPA
                     GSRRHQRNAAWVRVAKTERREFSGPVYSLEVPDSHTFVTTGGLTVHNCFPKDVSALKQ
                     LAGNTGYHFQLLTAVIEVNELQKRRTIGKLMKHLGSLVGKEVALLGLAFKPNTDDIRE
                     ATSLVLAARLQSEGAHVRVYDPVATARAAELLGGARVCETAEEALDGADGAVLVTEWP
                     EFRELDWEGAIKDKMARPLVVDGRNFLDAGLLENAGYAYEGIGR"
     gene            7224..8297
                     /locus_tag="VF517_15275"
     CDS             7224..8297
                     /locus_tag="VF517_15275"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX8054349.1"
                     /translation="MQALVLAGGEGTRLRPLTLEVPKPAMPLAGRPFLTFMLDWLAGH
                     GVTEAILSCGYRSDGVRDVLGDIYDGMRLRYVIEEEPLGTAGPVRLAADEGVLTDRFL
                     VLNGDTLTDIDLGAEIAQHAERGAQGTLALYGVDDTASYGVVPTADDGRVIEFLEKIE
                     NPPTNRINAGAYVLELSVAERIEPGRSVSFEREIFPAMAAEGVLYGYMAEGYWIDIGT
                     PERYLEATYDLLARRVGSRLPASDETGSLIHDGSITAGAHIGPQTVVGAHCVVGEGSV
                     VARSVLHQSVRIGAGCHVESAVLAEGARVDDGARIEPGAMVGAGAVIAAGAVVPRDAR
                     VAPRETVAAAEAPAPVAHEDRAL"
     gene            8294..9349
                     /locus_tag="VF517_15280"
     CDS             8294..9349
                     /locus_tag="VF517_15280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEX8054350.1"
                     /translation="MTGDPASAGESGSAEPAPDRDPSGSAEPAPDRDPSGMLDDVLAQ
                     PAQIDDALWRFEAADVPREPRPGGLGVAGLGGAARGGDLAAAAIGDRATAQVRTRRGY
                     GLEPWARPDALVLCASYSGDTEETLATYASARELGAPRVALTTGGELSRLAREDGVPV
                     IGIPSGMQPRAAVVYMTVAALECAAACGASPSLRSEIEASAATLRAVANDDAPARELA
                     AALRDRVPVVYGAGRTAAPAIRWKTQLNENAKVAAFANELPEADHNEVCGTDERLAAI
                     FLDDDDLDPRLRKRIEVTEAVLGGGHRVSARGTSPVERVLSLVLLGDLVSVHLAYERG
                     VDPTPVDVITELKSKLA"
     gene            9452..10150
                     /locus_tag="VF517_15285"
     CDS             9452..10150
                     /locus_tag="VF517_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054351.1"
                     /translation="MKHWLHRLTWWSGLQLLAAITGIAGFVVTLVGDLSRTQRLTLGI
                     ITVCAVTAIALTALLTRRRPLLPVKREEMVAKGRSLLAHEIKYSAVLFAGDMSWAPEY
                     CGPIADAVARGKQVTVIYPRDKTGAAISHNAAMLGEVGARVVPVITDTRLRAMLIDRE
                     NPDQALLFVVNPRRDMHVRDFGHSTTGDVYEGKVYAWKEDRVLIATATKLYEVLDPAG
                     ASSDSSGSPQAPSG"
     gene            11094..11432
                     /locus_tag="VF517_15290"
     CDS             11094..11432
                     /locus_tag="VF517_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8054352.1"
                     /translation="MHQAAETTGWSPRMLRYVERVGLVETSRNGSGYRVYGPGELQRL
                     RTLRELLARFDCTLSDIAFAKRMRGEDGLEEALASWFEAEPRQPEGVAPSDWLRFEQE
                     KHQRLLALAS"
     gene            11474..12940
                     /gene="ahcY"
                     /locus_tag="VF517_15295"
     CDS             11474..12940
                     /gene="ahcY"
                     /locus_tag="VF517_15295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX8054353.1"
                     /translation="MTTTLTPDTASKIDFKVADLGLAEFGRKEIRLAEHEMPGLMKTR
                     EEFAASQPLKGAKIMGSLHMTVQTAVLIETLTALGAEVRWVSCNIFSTQDHAAAAAVV
                     GPDGTPEDPRGVPVYAWKGETLEEYWWCTERALDWPGGDGPNMILDDGGDATLLVHKG
                     AEFEKGGEVPYPAKTDPEEYGYILETLRRTIAEDGQRWTRIAEQIKGVTEETTTGVHR
                     LYQMQEAGELLFPAINVNDSVTKSKFDNLYGCRHSLVDGINRGTDVMIAGKTALICGY
                     GDVGKGCADSLRGQGARVLVSEVDPICALQAAMEGYQVVRLNEVVGDVDIFVTATGNK
                     DIITAEHMASMKHQAIVGNIGHFDNEIDVAGLADRDDVQKINIKPQVDEWVFSDGHSI
                     ILLSEGRLLNLGNATGHPSFVMSNSFTNQTIAQIELWTKNDQYERKVYVLPKHLDEKV
                     ARLHLDSLGVKLTQLSPEQADYIGVPVEGPYKPDHYRY"
     gene            12944..14104
                     /locus_tag="VF517_15300"
     CDS             12944..14104
                     /locus_tag="VF517_15300"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX8054354.1"
                     /translation="MDRTRVQWAGAHMPVLRGVGERFARERPFDGLVVGACLHVTAET
                     ANLVQVLTAGGAEVALCAANPLSTQPDVAAVLEGDGVEVHADPASYERNIDAVLARGP
                     RVTMDDGADLALRVEGDVLGGTEETSTGALRLRAAKDLAFPVIAVHEGRTARLFDTRY
                     GTGQSTLDGILRATNVLLAGRTVVVVGFGGCGRGVALRAKGLGAHVIVCEVDPLAALE
                     AAMEGYRVMPAAAAAAEGDVFITATGTRDALTREHFEAMKDGAILANAGHFDVEIDKP
                     ALAELSGAVREVRPLVAEHVLAGDRRLYLLAEGRVVNLASAEGHPAAVMDVGFATQAL
                     AVERIVRGDLAPGVHGVPDEIDREVARLELAALGVRIDELTDAQREYLHSWG"
     gene            14178..15125
                     /locus_tag="VF517_15305"
     CDS             14178..15125
                     /locus_tag="VF517_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX8054355.1"
                     /translation="MKAMVLAAGLGTRLRPLTYEIPKPMVPVLDRPVMAHILGGLRRQ
                     GFDEVIANLHYFPDAVKGYFGDRVEYRYEEELLGTAGGVRNVADFFGDDPVVVISGDA
                     LTDVDLNALVERHRQAGGIGTLTVKKVPDTREYGVVIHDSEGRIQGFQEKPEPAEALS
                     DLGNCGIYCFSPEIFDYFPEEPFADWAQDVFPALLENDVPFHAYETEDYWNDVGSLAE
                     LREGTWDALAGRLKIEVTGATDGDPPVWTGHDVDIGSDVRLMGPVAIGDRCRIGDGCA
                     LRDTIVFPGTEVAPGTIAIGAIIGRGGIVEAMRPLDELE"
     gene            complement(15246..16226)
                     /locus_tag="VF517_15310"
     CDS             complement(15246..16226)
                     /locus_tag="VF517_15310"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX8054356.1"
                     /translation="MSELLDRLGVERPVVQAGMGGGISRARLAAAVSGAGGLGTIGFL
                     PPGELRREIAAAREQTERPVAANLLLPFAGRAHAEAAAEADAVVTFWGEPRRPGSARV
                     WLHQAGSLAEARAAREAGADGVIVQGREAGGHVRGKLPALELLAQARAELPAPYPLLL
                     AGGIATADDVRTALDAGADAAVLGTRFVMTPESGAHPAYRERLVEARETVLTELFGMG
                     WPDAPHRVVENEAVRRWRRRERPIGALNRLTGRLMPAAPTGLQERLIAAQRPAIPLLS
                     PYAPTDDRGENLLESGPLYAGETVARIGDVRSAAELVRELAPPTAQPRPA"
     gene            complement(16223..17053)
                     /locus_tag="VF517_15315"
     CDS             complement(16223..17053)
                     /locus_tag="VF517_15315"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX8054357.1"
                     /translation="MSAASHPQLHWSLEIGPLAALAVGIFLYARRFRAARREAGGRGA
                     GPLQATCFGLSMLILFAALISPLDRLGEDYLFVAHMAQHILLGDLAPLLLLLGLSRVI
                     MRPATRRLHGVERRLGALASPPAFILLWLALVYTWHIPALYVGAIHHAPVHALEHMTF
                     FTAGTLVWWPLIQPVPMRRRLTGIAPFAYIFSAKIGLAALGIYLTWSSNLLYDHYGTV
                     PRIWGLTPIEDQNVGGAMMMVEQSIVLVIVLVFLFGRMLVQSEEEERRRERLEDAAAA
                     "
     gene            17115..17864
                     /locus_tag="VF517_15320"
     CDS             17115..17864
                     /locus_tag="VF517_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054358.1"
                     /translation="MALARLIGLLAPRLCASCGAVAGDAEPLCGRCRHALAWLGPAAV
                     ELAGVRVWAPVSYAGPARDLVRALKFGGAWRAAETMAAQIAANAPPELLGDRAGRSNA
                     RYTGLGSTSSPAEGRPELLPGPAHAARAEGPAALVPVPLHPRRRRRRGYDQAALLARA
                     LAERTGLPVRACLDRSGAAGAQVGRGRAERLGGPPGEIRLRPGAEAPRAALLVDDVVT
                     TGGTLASCAAALRAAGTADLAALAYARTPGR"
     gene            17907..19544
                     /locus_tag="VF517_15325"
     CDS             17907..19544
                     /locus_tag="VF517_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054359.1"
                     /translation="MRLATITALAAGLALALPASGQAKNLCVGAPGGACDSSHASIQD
                     AMNVASDAGHPGRDTIRIGKGLFEAPPISQPNAAQNPVDVVGSGQDIDGTRLRSTTAQ
                     PAIRLTTASTIDNVRILIPPAGVGGLDLEAADARNVTVFAEPGDGAVTGKTGVYMRKD
                     SSLRNSTVSLVTAPAPSYAVSITDAGNHVIEDALLDGTDLVDLTGYATQATIRRIRNS
                     GRCSSCLYLRPAAPDKTVDISDSLLRVAAEGQGLYAQPGTTINARNNTIVGVPGTNTA
                     GARANGSGQVNVLDSIIRDVNYALFTDGSAHLSIDYSDFDPDKLQKVGVAGLFSIGAH
                     NVNADPKFMNADVGDYRLASGSPVAERASAGAGAGDLDLERKPRLADADGDGTPIRDM
                     GAYEYSGAPYALPPVEYIPHGDGGGGGGGTIQGPVMTFKSKTVKLDRKRRVKVGLTCP
                     HSEIGLCSGRVRLVSTKKIRPKPGKKAVYVGVGAADFKSIPADTTRTVTIKVSKKTAA
                     ILRRFKSVKVTGTIVASWQTGDPRTSTYPLTLKPSTK"
     gene            19588..19932
                     /gene="raiA"
                     /locus_tag="VF517_15330"
     CDS             19588..19932
                     /gene="raiA"
                     /locus_tag="VF517_15330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX8054360.1"
                     /translation="MRIEVKGRNGVVVDDDLRARIEKRFEKVARQVSELAVLEVELSE
                     EKNPSIKDRQVAEATLRLKGTTLRARDASEDLSHAIHLTCDDLARQVKKHRDKRRGRR
                     HAASAEAPPAAP"
     gene            19954..22803
                     /gene="secA"
                     /locus_tag="VF517_15335"
     CDS             19954..22803
                     /gene="secA"
                     /locus_tag="VF517_15335"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX8054361.1"
                     /translation="MSILDRALRIGESKKIKGFQQRVARINDFEAELALDDDHELLER
                     ASSLRERARNGEDLDRLLPESFALTREAAKRALGQRHFDVQLIGGMVLHDGSIAEMRT
                     GEGKTLTATLPVVLNSLPGKGVHLVTVNDYLARRDAEWMRPVYEMLGVSVGVVQSDER
                     DAAKHAAYASDVTYGTNSEFGFDYLRDNLATSLEDQYQRGVHNFAIVDEVDNILIDEA
                     RTPLIISGQPEQAADVYYKFAALAKRMVPGKKPEGMEAKSKEYEADFDFEPDEKHKTV
                     AVTERGVAKAEKFMGIENLYLAEHGNLVNHLIQSLKAEALYKRDVDYAVIDGEVKIID
                     EFTGRILEGRRWSEGLHQAVEAKEGVPIQEENQTVATITYQNYFRRYDKLGGMTGTAL
                     TEATEFMKIYELQVVEVPTNRPMVRKDQNDQIYKTKDGKWAAVVNEIEARHEKDQPVL
                     VGTISVEVSELLSKQLEDRGIEHAVLNAKPEHAEREGEIIAEAGRLGAVTIATNMAGR
                     GVDIKLGGNAEHQTHQELLKLGLDPDSNDWDEEWNALYPRIEAKVEEDREKVKEVGGL
                     YILGTERHESRRIDNQLRGRAGRQGDPGESRFYLSAEDDLVRLFAGDRIYRILDRLGP
                     VGEEGEEQPIEAGMLSKQIENAQKKVEEQNFLIRKRVLEYDDVMNQQREIIYEIRQRI
                     LKGEDMSETARTQIADAIERMTDEYLVGDFVEDWDVDALLTQIEQIYPVAIAPDDLDP
                     NTIERSALIAELREDVVKAYDEREEELGSDLMRQLERFILLQIIDDRWREHLHDMDYL
                     REGIHLRGFAQEEPLVAYKNEGFGMFTTLVNSIWEEFGRYIFNVEVEIEGEEAQGQAS
                     NWQPSASSSSTGNLQYQGGRPEDQPSALQAAAAGALPVAAADAAVSPDSNGAPEEAAA
                     AASVETRRLGEHEQIGRNDPCWCGSGKKFKKCHGA"
     gene            22863..23888
                     /gene="prfB"
                     /locus_tag="VF517_15340"
                     /pseudo
     CDS             22863..23888
                     /gene="prfB"
                     /locus_tag="VF517_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            23891..24709
                     /gene="nadC"
                     /locus_tag="VF517_15345"
     CDS             23891..24709
                     /gene="nadC"
                     /locus_tag="VF517_15345"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX8054362.1"
                     /translation="MQELIDRALAEDVGSGDLTTEALVAPESRSAARIEQKAPGVVSG
                     LRVAESVFRRVDPELRWHGHAAEGEWRDGGLVAEIAGSTASILRGERVALNFLGRLSG
                     VATLTARYVHAVEGTGARILDTRKTTPGLRALEKAAVADGGGVNHRFGLFDAMLVKEN
                     HIAAAGGVGAATRLALTAAGEGVPVEIECASLAEVREALEAGAPRILLDNMTLDELRE
                     AVALTEGRAELEASGGVTLETVRAIAETGVDFISVGALTHSAPALDLSLLLDPL"
     gene            24706..26544
                     /locus_tag="VF517_15350"
     CDS             24706..26544
                     /locus_tag="VF517_15350"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8054363.1"
                     /translation="MIATFLPWRDSVRLIQESRRKALVLAALLIAAVLASAPPASAQS
                     DPLSALRTSCAAKKSPDGARYRICTAQVASFDGTPLDVTLTLPAKRSKRRVPLVAFLH
                     GFLSQKGEYLSETREGTGPDRGADAYKTVRWNNVWFASRGYAVLNYTARGHGDSGGQI
                     GLASRDVEVRDTQYLTGLLVDRAGPLARIDRRRVGVIGGSYGGGQTWLLMTTRPSASL
                     PYGTWLSPAGRRVELAAVVPMYTWSDLLYALVPNGRHLSSGVDPRTAASPIGIAKLTL
                     LDGFLATAGPRLPQQVYGWLARVNAGEPYEGGDPLLEEARRELTVTRSGFYQDGYFEA
                     LRRRKVRRVPVLAAQGWTDPLFPAIEVLRMYRRLKASSPGYPIQLYLGDFEHLTARSK
                     VADLRHFHVLGNRLLDRHLRGRGPKPVLDARAAVTNCDEKRFGPVLKAKTWDGLAGPA
                     LSFDLGGPKQTTARAAGQGPATDPIVLAQQRGRGCITTTAADSPGSARYSIPLTAPAT
                     LLGLPRVTVTYTATAPDFELNAHLWDVAPDGTRTLIDRGAYRGGPVLSGTADYELFGN
                     AWRVEAGHQLELELLQDDSTFLRPHNYPSTVTVQNARLGLHLAAPG"
     gene            26587..27606
                     /gene="nadA"
                     /locus_tag="VF517_15355"
     CDS             26587..27606
                     /gene="nadA"
                     /locus_tag="VF517_15355"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX8054364.1"
                     /translation="MQLPTAPSGATIDAPLALEDIPALQREVRALAEQRNAVILAHNY
                     QVPEVQDVADFVGDSLALSRKAAAAGADTIAFCGVHFMAETASILSPDKTVLLPDLGA
                     GCSLADSITADQLRTWKAQHPGAIVVMYVNTTAEVKAETDYCCTSSNAVQVVEHIRRE
                     HGEDVEILFGPDMFLGAYVERVTGRPMQVWAGECHVHAGIRPSDIAKVRAENPGAEFL
                     IHPECGCSTSVMEYVAAGDVDAENTHMLSTGGMLEHARSSDADTFIVATETGMLHPLE
                     QENPGKRFVAANSCAVCPFMKMITLPKLRDALRDGGPRYTVKVDAELAERARVPIERM
                     VAIGG"
     gene            complement(27729..>28113)
                     /locus_tag="VF517_15360"
     CDS             complement(27729..>28113)
                     /locus_tag="VF517_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054365.1"
                     /translation="SATGTLTINVSGGGTLTGLVDSTTRIECEDRSGVTSASSGPGRD
                     GDGSDDNSGPSSNSGPGSTTSDRGDDDRGDDDARDDDRNDDDRDDDDRVCTTADLTPG
                     TVVHEAEVELRDGKAVFEEVELLKP"
BASE COUNT         4427 a        10059 c         9887 g         3740 t
ORIGIN      
        1 tcgagcgcat cgcgcccggc gcgggcactc tgcacgtggt gccgctcggc atcgacctcg
       61 acgcgatcaa gcgaaccacg ccctacgacc cggacggccc ggtcgtcgcg gtggggaggt
      121 tggtcgagca caagggattc gccgacctcg cggctgtggc cgggggcgtg ggacgaggcg
      181 tggtggtagc gggggagggg ccacagagaa aggagctgga gcacctcgcc gccggtgcga
      241 tgacgttccg cggggcgctc tccccagcag cggcgcgaga cctcatcgag accgcgtcgg
      301 tgctggttgc accgagcgtc atcgcgaccg acggctcccg cgacggcatc cccatggtca
      361 tcaaggaggc cctggcgctg gaaacacccg tcgtggcgag cgacgccgtc ggcaaccccg
      421 aggtcgtgaa gcccgagcac ggtgctctgc acagagcggg ggacaggcag gacctggcgg
      481 agaagatccg gctgacggcg gggcgcgaag ccctgggcaa ggccggccgc gccttcgccg
      541 agcggcacgc cgacctgcgc acccagacgg cccgcctcgc ggagctgttc agacgcccgt
      601 gagcgcctgg ctcgtgccgg actcgtccgg gatctcgtcg acctgcccca ccgcgcggaa
      661 cgccagcgcc accgaccccg ccgccagcag catgttcacg cccagcaagc caagcgcgat
      721 cgcacgcagg tcctcgccgc cgatggcgat catcgccgcc ggctgcaccg ccgcggcggc
      781 cgccaggatt ccgatgaacg cggcccggtg cagggcgagc tggtactgca ccgccaggta
      841 ggtcagcgcc agcagtgaca tcgccagccc gagccatggc agcgcctcgg cggcgcccgt
      901 cagcttgaac gccacgcgca gcacgatctc cccggccacg gcgtacacga tcaccatcgg
      961 cgcggcgatc gccaggatca gcccgagcgt ccgcgccagc acgcccgacg cccgctcgcc
     1021 gaggtgggtg cgccgcgctg cctccgggac caggtacagg gacagcccga ccgccaccca
     1081 catgatcgcc ttggcggcga ccgcggccgc ggcgtaatcg ccggccgcgt cgtccgaggc
     1141 gaggtgcttg acgacgatca cgttcccgtc ctggagccac gccacgagac cgagggccac
     1201 gaccggcacc gccgcccgcc gcaccagctc gcgcagcggg tgctcgtgtg cgtgccgcgc
     1261 gttcggcagg ttgcggcgca gcgggatgag cagacagagc ccgaccacca ccagcgccag
     1321 cggcgtgccg aggaacgccg ccgtgatctc cgcctcgccg ccgctcacgc ccgccacgag
     1381 cagcagcccg agcacgagtc gcatgaactg ctcgagggcg aggctcaccc cgaccacctt
     1441 gtactcctgg aaccccagca gcgccccgcg ctccaccgac aggacgagcc agaggagccc
     1501 cgtgggcagg gtcgcggcgg ccgcccacgg caggtcgtgc acgccgatca cggcggcgag
     1561 cacgtcgcgc gcgagcaccg acaccaccac gatcacgacg cccgcgacga gcagccgccc
     1621 gagccagcgc cgcacccccg ctcccgcggc cgggtccccc gccgccagct cggcgctgat
     1681 ctcgcgcgcc accgtcgtct ggagcgcctg gcccgggacc atcaggatga tgaacgacga
     1741 cagcagcgcc gccagcgagc cgtaccccgc cccgccgagg atccgcgcga agacgaccgt
     1801 gaacgccagc gccatgacgt tgccgacgat caccgccgcg gcgaggccgg ccgcgcggcc
     1861 cgtgtcggag ccgatcatcg tgcccaggcg gcgagcgtct agagaccgga gcgaggcgcg
     1921 caaagcggcg caaggctatc cccgatttcc cctcgggtac cgccgttatc ctccgccgtc
     1981 gtgcaggaac gcgccctggc cgagcggctg atcacgtacg acacgtccga gctcgacggc
     2041 attcaggcgg cggccggctt cgtgaagggc tggctcgaat cgaacgacgt cgagatcaag
     2101 gacggcaccc acaacgatct tccggtgctg gccgcgacgg tcggcccgga ggagggcccg
     2161 acggtcgtgc tgcacggcca catcgacgtc gtgcccggcg cccccgagca gttcgagccg
     2221 cgcatcgacg gcgaccgcct gatcggccgc ggggcctacg acatgaaggg cgggctcgcg
     2281 gggatgatgt gcgcggtccg cgacctgaag gaccagaccg acgtgcgcgt gcacttcgtc
     2341 tgcgtctccg acgaggagtc cgacgagccg ttttccaggg gctccgacta cgtcgtcgaa
     2401 aagggctaca taggcgactt cgcgatcacc ggtgagccga cggacctgcg catcggcgtg
     2461 caggccaagg gcgtgctggc gctgcgcatc gaggtgaccg gcgtctcggc ccacggctcg
     2521 accccctggc tcggcgacaa cgcggtgctg aaggccgtcg acgtcttccg ccagatccag
     2581 tcgatgccgt tcgcgcggga ggcgagcgac ctgttcgacc gcccctcgat cagcctcggc
     2641 cgcatcgccg gcggcgaccg catcaacacc gtcccgggcg cctgcaccgt cgacgtggac
     2701 atccgctacc tgcccggcca ggacccgcgc gcgatcctcg ccggcatccg cgcgctgccg
     2761 gacatcgagg tcagcgtggt gctccagcgc gacccggtga acgtcgagcg cgacaacccg
     2821 ttcgtggtgg ccctcgcgga cacgctctcc gagtggctcc cggccgaccg cgtctcgatc
     2881 ggccgcgacg gcgcctccga tgccatcagc ttcatcgacg ccggcgtccc ggcggtcgag
     2941 ttcggcccgg tcggcggtgg ccaccacggc cccgacgagt gggtctcgat cgagtcgctc
     3001 gagcgctaca gccgcgcgct cgtcacgttc atcagcgagc tcccggccag cgccgggaag
     3061 gggcacctcc gcattgcctg accccaagcg cggcatcttc gggcggtacc tgatcggcgc
     3121 gctgctgatc gtgctcgcct ccgccggcgc gaccggcgtg gcggcgttcg aggagctcga
     3181 ctcggtcgtg aacgcgttca ggcgcggcca gacgctcgag ctcgacgacg agctccagaa
     3241 ggccgacgcc ggcaagccgc agacgatcat gctgatcggc agcgacaccc gcggcaaggg
     3301 ctcgcgcgac tacacgggcg agcgcggcct ctcggacacg atgatgctcg tccgcctcga
     3361 cccgtcgaag aaccagatcg cggtgctctc gctcccgcgc gacctcaagg tgaggatccc
     3421 gggccacggc gtcgacaagc tcaacgccgc ctatgcgctc ggcggtgcga agctcacgct
     3481 gaagacggtc aaggacttca ccggcctcgg cgtgaaccac gtggtcaacg tcgacttccg
     3541 cggcttccgc aaggcggtca acgcgctcgg ctgcgtgtgg gtggacgtcg accgccgcta
     3601 cttcaacgac aacagcggcg gggaccagta cgccacgatc gacatcaacc ccggctacca
     3661 gaagatctgc gaccaggacg cgctcgacta cgtccgctac cgccacaccg acaacgacct
     3721 cgtccgcgcg gctcgccagc aggacttcct gcgctcgctg aagagccaga tcggcgtgaa
     3781 ccggatcgtc cgggaccgca aggagctcat ctcgatcttc ggcaagtaca cgcagtcgga
     3841 catcggctcg cgcaagaccg tgctccggct gctcaagctc gtggtcgcgt cggccgggct
     3901 gccgatccat cagattcact tcaaggcgaa catcggggcg agctacgtga cggccacctc
     3961 cgagcagaca cacgagctcg cggacgagtt cctgggcgtc gagggcccgg cgccggaccc
     4021 gaccaagccc ggcaagaaga ggaagcgcaa gaagcaggct gacctccggc tcacggactc
     4081 ctcggtcatg ggcaagcaga tggccctcca gctcgtgaac gagggcctgc gcgggatcga
     4141 gatcgcgtac ccgcgcaagc tccccgcggg ggcgtcgttc caggccccgc cgcggtcgta
     4201 cacgatcagg gccggccagc ggaagaagaa gcaccgctcg ttccggatgg tgatctccgc
     4261 cggccgcatc ggcgagtact acgggctcca gggcacgacc tggaagaacc cgccgatcct
     4321 ctcggggccg cacgagaacc gcgaggtggg gcggcagaag gtgcaggtct actacgacgg
     4381 gccgcgcgtg cggctggtcg cgtggcgcag cggcggggcg acctactggc tgtcgaactc
     4441 cctgctccag cggatgaacc tcgacgagat gctcgcggtc gtccgctcga cgcggccttt
     4501 gtagcctcat ccgccccgtg gccgagcgcg aacccatcgg agtgatcggg gccggctggg
     4561 tcggcctggt cacggctgcc tgcttcgcgg agctgggcca cgaggtccac tgccgcgaca
     4621 tcgtccagga gaaggtggac acgctgcgcc gcggcgaggt gccgatctac gagcccggcc
     4681 tgcccgagct gatcgagaag aacggcgagc gcatcaactt caccaccgac atgggcgagg
     4741 tgctgagcgg tgcgaacctc ctgttctgct gcgtggacac gccgccgacc tactcgggcg
     4801 acgccgacct ctcgcgcgtc gagcgggtgg tcgaggagct gggcgactcg cgcgaccacg
     4861 cgatcgtcat gaagagcacc gtgccggtcg gcaccgggcg ctcgatccgc cgccgccgcg
     4921 aggacctcgg ctacgtgtcg aacccggagt tcctcaagga gggctccgcg gtcgcggact
     4981 tcatgcaacc cgaccgcgtg gtggtgggcg cgggagatga gtcgcagggc ttcgcccacc
     5041 gcgtggccgc gctgtacgag ccgctcgact ccccgatcgt gcgcaccgac gtggccagcg
     5101 ccgagatggt caagttggcc tcgaacgcct tcctcgccac gaagatctcg ttcatcaacg
     5161 agatcgcgaa cgtgtccgag gagctgggcg cggacgtgac cgaggtcgcg cgcgggatgg
     5221 gcctcgacga gcggatcggc acgcagttcc tgcgcgctgg gatcggctac ggcggctcgt
     5281 gtctcatcgg cgaggaaacc gtgatgatcc ggcggcgcgg catgtgcgcg ctgatcgcgc
     5341 tcgaggagct gttcgagcag atcgccgacg agcaggatct cgatcacctc gatccggagg
     5401 tcctgcatcc cgacgggcta gaggttctat cgtggcgcga gggcaggccg cgaccggagt
     5461 tcctgccggt gccggtgctc acgcgccgcc actacgaggg cgatgtcgtg gacgtcacga
     5521 cgaagatggg gcgccgtgtg accaccacgg cggatcaccc gttcgtcgtg gcggataccg
     5581 gcggcgccga ggtccggacg aagctggcga gcgagctgag cacggaggag gattggctgc
     5641 caattgcgca gggggctcct atcacggccg tcgaggacgt ccagctcgag ccgctcgagg
     5701 gtgccgtcat ggccgagctc cagcccgcgg acgtgatcgt gcggctcgcc gagcccgagc
     5761 tcgctgctct cgcggccgcg ccgcgcgagc gttgggtttc cgggctcgac cacccgcgcg
     5821 gggtcacgca gcgccgctcg gacatactcc gcaggggggc gttgcgactt cacgaggcga
     5881 accgcctcgg ggcctcgacc cgcggcgccg cggcactgac ggcgcgcaac ggcaccgcga
     5941 tcccgttcgg cctgcggctc gacgcggcct tctggcgcgt ggtcgggctc tacatcgccg
     6001 aggggcacat gagccaggga catcgcatcc actggtcgtt ccatccgacc gacgaaaccg
     6061 acctggtcga ggaggtgcac gacttctggg aggcgcgcgg ggtcaaggca tccgtgttcc
     6121 gccaccccac cgccaccgag gtccgggtgt cctcacgtct tctcgccggc tggctcgagg
     6181 cattcggcct cggccgcaac gcctacgagc agcggatacc cgacgccgcg tggagccagg
     6241 acgaggcgac caagcgcgcg ctggtggcag gcatgtggcg cggcgacggc tcgtggtcct
     6301 tcgttcgcgg cgggccgagc gtagtgctcg agtacggcac catcagccgc gagctcgccg
     6361 acggactgct tcggttgctc ggcgacctgg gtgtcgtcgc tcgtctgaag gtcgggcgga
     6421 cgaagaagtc gacggccgat acgtattggc tggtcgtctc gggcgcccag caggtcgagc
     6481 ggctgctcga ctgcgtcccc gcggccgacc gtgggcagat ccgagcgtca ctcgctcgcc
     6541 aggccaagcg catcgcgcct gctggttccc ggcggcatca gcgcaacgct gcgtgggtca
     6601 gggtggcgaa gacggagcgc cgcgagttct ccggtcccgt ctactcgctg gaggttccgg
     6661 actcgcacac gttcgtcacg accggtggcc tgacggtgca taactgcttc cccaaggacg
     6721 tctccgccct caagcagctc gccggcaaca ccggctacca cttccagctc ctgaccgcgg
     6781 tcatcgaggt caacgagctc cagaagcggc gcacgatcgg caagctcatg aagcacctgg
     6841 gatcgctcgt gggcaaggag gtcgcgctgc tggggctcgc cttcaagccc aacaccgacg
     6901 acatccgcga ggccacgagc ctcgtgctcg ccgcgcggct ccagagcgag ggtgcgcacg
     6961 tgcgggtgta cgaccccgtc gccaccgccc gcgcggccga gctgctcggc ggcgcgcgcg
     7021 tctgcgagac cgcggaggaa gcgctcgacg gcgccgacgg cgccgtgctc gtgaccgagt
     7081 ggccggagtt ccgggagctc gactgggagg gggcgatcaa ggacaagatg gcccggccgc
     7141 tcgtggtgga cggccgcaac ttcctcgacg ccgggttgct agagaacgcg ggctatgcgt
     7201 acgagggcat aggtaggtag ttcgtgcagg cgctggttct cgccgggggc gagggaaccc
     7261 ggctgaggcc gctcacgctc gaggtgccga agcctgcgat gccgctcgcg ggccggccgt
     7321 tcctcacgtt catgctcgac tggctcgccg gccacggcgt gaccgaggcg atcctctcgt
     7381 gcggctaccg ctcggacggc gtgcgcgacg tgctcggcga catctacgac ggcatgcggc
     7441 tgcgctacgt gattgaggag gagccgctcg gcaccgccgg cccggtgcgc ctggccgccg
     7501 acgagggcgt gctcaccgac cgcttcctcg tcctcaacgg cgacactctc accgacatcg
     7561 acctcggcgc ggagatcgcg cagcacgccg agcgcggcgc gcaggggacg ctggcgctct
     7621 acggggtgga cgacaccgcc agctacggcg tggtgccgac cgctgacgac ggccgcgtga
     7681 tcgagttcct cgagaagatc gagaacccgc cgaccaaccg catcaacgcg ggcgcgtacg
     7741 tgctcgagct gtcggtggcg gagcggatcg agccgggccg gtcggtctcg ttcgagcgcg
     7801 agatcttccc ggcgatggcc gccgagggcg tgctctacgg ctacatggcc gagggctact
     7861 ggatcgacat cgggacgccg gagcgctacc tggaggcgac ctacgacctg ctcgcgcggc
     7921 gggtggggag ccggctcccg gcgagcgacg agaccggctc gctgatccac gacggatcga
     7981 tcaccgcggg cgcgcacatc gggccgcaga cggtggtggg agcgcactgc gtggtggggg
     8041 agggctcggt ggtggcgcgc tcggtgctgc atcagagcgt gcggatcggg gccggctgcc
     8101 acgtcgagag cgccgtgctg gcggagggcg cgcgggtgga cgacggcgcg cggatcgagc
     8161 cgggcgcgat ggtcggcgcc ggcgcggtga tcgcggcggg cgcggtggtg ccgcgcgacg
     8221 cgcgggtggc gccgcgcgag acggtggccg cggcggaggc gcccgcgccg gtcgcgcacg
     8281 aggatcgggc gctatgaccg gggaccccgc cagcgctggg gagtccggca gcgccgagcc
     8341 cgccccggac cgcgacccct ccggtagcgc cgagcccgcc ccggaccgcg acccctccgg
     8401 catgctcgac gacgtgctcg cccagcccgc ccagatcgat gacgcgctct ggcgtttcga
     8461 ggccgcggac gtcccgcgcg agccgcgccc cggcggcctc ggggtcgccg gcctgggcgg
     8521 cgctgcccgc ggcggcgacc tcgccgccgc ggccatcggc gaccgcgcca ccgcgcaggt
     8581 ccgcacgcgg cgtggctacg gcctcgagcc gtgggcgcgc ccggacgccc tcgtgctctg
     8641 cgccagctac tccggcgaca ccgaggagac cctcgcgacc tacgcctccg cccgcgagct
     8701 gggcgccccg cgcgtggcgc tcaccacggg cggcgagctg tcccgcctcg cccgcgagga
     8761 cggcgtcccg gtgatcggca tcccctcggg catgcagccg cgcgccgcgg tggtgtacat
     8821 gaccgtggcc gcgctcgagt gcgccgccgc ctgcggtgcg agcccgtcgc tgcgctcgga
     8881 gatcgaggcg tccgcggcca ctctgcgcgc ggtcgcgaac gacgacgccc ccgcccgcga
     8941 gctcgccgcc gccctgcgcg accgcgtccc ggtcgtctac ggcgcgggcc gcacggcggc
     9001 gcccgccatc cgctggaaga cccagctcaa cgagaacgcc aaggtggccg cgttcgcgaa
     9061 cgagctgccg gaggccgacc acaacgaggt ctgcggcacc gacgagcgct tggccgcgat
     9121 cttcctcgac gacgacgacc tcgacccccg cctgcgcaag cgcatcgagg tgaccgaggc
     9181 cgtgctgggg ggcggccacc gggtgagcgc acgcggcacg agccccgtcg agcgcgtgct
     9241 ctcgctggtc ctgctgggcg acctggtctc ggtgcacctc gcctacgagc gcggcgtcga
     9301 ccccaccccc gtggacgtca tcaccgagct gaagtcgaag ctcgcttagg cgtgttcgtt
     9361 tccgccagac ccagacagcg ctcgaggcct atatcgcata gcgtcccttc acgatgctgg
     9421 cgggccacac gagacctggg agggagtaaa cgtgaaacat tggctgcacc gcctcacctg
     9481 gtggagcggg cttcagctac ttgcggcgat cacgggtatc gcagggttcg tagtcaccct
     9541 cgtgggcgac ctgtcgagaa ctcagcggct aacgctgggc atcatcaccg tctgcgcagt
     9601 caccgcgata gctctgacgg ctctgctcac gaggcgaagg ccattattgc ccgttaagcg
     9661 agaggagatg gtcgcaaaag gtaggtcgct gcttgcgcat gagatcaagt attccgccgt
     9721 cctcttcgcc ggagacatga gttgggcacc tgagtactgt ggccccatcg ccgatgcggt
     9781 tgcgcgcggc aagcaggtca ccgtcatcta cccccgggac aagaccggtg ccgctatcag
     9841 ccacaacgcc gcgatgcttg gcgaagtcgg cgcgcgagtc gtgcctgtca tcacagacac
     9901 ccgacttcgc gcgatgttga ttgaccgtga gaatcccgac caagctcttc tcttcgtcgt
     9961 caatccgcgg cgcgacatgc acgtcagaga cttcggccat agcacgaccg gcgatgtgta
    10021 tgaaggcaag gtctatgcct ggaaggaaga tcgggttctc atcgccacgg caacgaagct
    10081 ctacgaggtc ttggatcccg cgggtgcttc aagcgacagt tctgggtccc cgcaggcgcc
    10141 ttctgggtaa ctagggcctg gacggatatg ccacctctgg acggatatgc cacgtctcag
    10201 gcgccttctt ccgagtgcct tcttgacggg acgccaagcg gggcgcacat tggagcgatg
    10261 gccaccctgt caggacacgg gttttaacgg tggctcgacc cggccccggc atctcaagtg
    10321 ggtacgccga gtcccctccg cccccagaac tcacagtctc tccgtctagc tacctggcgc
    10381 ccctaagtga cttgatcgat gaagacaccc tgcaccgttt cctggtgcgg tgtacgcagg
    10441 cgatcagccc gttctacgag agggccgaga tccgcttgaa ggtcgtttcg gttgcagaaa
    10501 cgttttcctt ggtgagcagg gtcgaacagc gtaggctcaa gcgtgccggg gaccttctaa
    10561 ggagcatgcg agcccgcggg gtcccgccgt actgcccgat cgcattgggc aatacggcgg
    10621 atagcggggt gtggctgatc tatccgccgc tcgtcgagcc ccaggcgaga cgaacctacg
    10681 tcatcaacgg tcaccaccgc ttgattgcag cacgcgactc caagatccgt cgcgtccagg
    10741 tgatcagcgt cgagagcgtg gtggagcgag cgccggcggc agggtcaagg tgggcggacg
    10801 tcgacactac gggttttctc agttcggctc gaaacgcaag ggttgccgat gtcaaggaag
    10861 gcctagtccg cccatccgca tccttctgca gaagtcgata tttctacttc gagtctcaag
    10921 agcacctcag agattggtgc gagtggttcg cgcgaagcgc agatcgttgg ctctggaatc
    10981 cggaaacaca aaagcttgag cgtgttacgg gtacatcagc cccaaatgca gacgttggcg
    11041 cacgtcaacc ttgacacagc cgtgctaacg ttgctcgcat cgacgggctg acgatccacc
    11101 aggcggcgga gaccacgggc tggtcgccgc gcatgctgcg ttacgtggag cgcgtcggcc
    11161 tcgtcgagac gtcgaggaac ggctccggat accgcgtgta cggaccgggc gagctgcagc
    11221 gccttcgcac cctgcgcgag ctgctggccc ggttcgactg cacgctctcc gacatcgcct
    11281 tcgccaagcg catgcgcggc gaggacggcc tcgaggaggc actcgcgagc tggttcgagg
    11341 cggagcccag gcagccggag ggcgtcgcgc cgagcgactg gctgcgattc gagcaggaga
    11401 agcaccagcg cctgctggcc ctcgcgagct gagcagcacg aacacgaccc gaccgcgtca
    11461 ggagaaacac acagtgacca ccactctcac cccggacacc gcctcgaaga tcgacttcaa
    11521 ggtcgccgac ctcggcctcg ccgagttcgg ccgcaaggag atccgcctcg ccgagcacga
    11581 gatgccgggc ctgatgaaga cgcgcgagga gttcgccgcg tcgcagcccc tcaagggcgc
    11641 gaagatcatg ggctcgctgc acatgacggt ccagaccgcc gtgctgatcg agacgctcac
    11701 cgccctcggc gccgaggttc gctgggtctc ctgcaacatc ttctcgaccc aggaccacgc
    11761 cgccgccgcg gccgtggtcg gccccgacgg caccccggag gacccgcgcg gcgtgccggt
    11821 ctacgcctgg aagggcgaga cgctcgagga gtactggtgg tgcaccgagc gcgcgctcga
    11881 ctggcccggc ggcgacggcc cgaacatgat cctcgacgat ggcggcgacg ccacgctgct
    11941 cgtccacaag ggtgcggagt tcgagaaggg cggcgaggtg ccctacccgg ccaagaccga
    12001 ccccgaggag tacggctaca tcctcgagac cctgcgccgc acgatcgcgg aggacggtca
    12061 gcgctggacg cgcatcgccg agcagatcaa gggcgtgacc gaggagacca ccaccggcgt
    12121 ccaccggctc taccagatgc aggaggccgg cgagctcctg ttccccgcga tcaacgtgaa
    12181 cgactcggtc acgaagtcga agttcgacaa cctctacggc tgccggcact cgctggtgga
    12241 cggcatcaac cgcggcaccg acgtgatgat cgccggcaag acggcgctca tctgcggcta
    12301 cggcgacgtc ggcaagggct gcgcggactc gctccgcggc cagggcgccc gcgtcctcgt
    12361 cagcgaggtg gacccgatct gcgcgctcca ggccgcgatg gagggctacc aggtggtgcg
    12421 gctcaacgag gtggtcggcg acgtggacat cttcgtgacc gccaccggca acaaggacat
    12481 catcacggcc gagcacatgg cctcgatgaa gcaccaggcc atcgtcggga acatcggcca
    12541 cttcgacaac gagatcgacg tggcggggct cgccgaccgc gacgacgtcc agaagatcaa
    12601 catcaagccg caggtcgacg agtgggtctt ctccgacggg cactcgatca tcctcctgtc
    12661 cgagggccgg ctcctgaacc tcggcaacgc gaccggccac ccgtcgttcg tgatgtcgaa
    12721 ctcgttcacg aaccagacga tcgcccagat cgagctctgg accaagaacg accagtacga
    12781 gcggaaggtc tacgtgctgc cgaagcacct cgacgagaag gtggcccggc tgcacctcga
    12841 ctcgctcggc gtgaagctca cgcagctctc gccggagcag gccgactaca tcggcgtgcc
    12901 ggtcgagggc ccgtacaagc cggaccacta caggtactag gcgatcgacc ggacccgcgt
    12961 tcaatgggcg ggcgctcaca tgcccgtcct gcgcggggtc ggtgagcgct tcgcccgcga
    13021 gcggccgttc gacggcctgg tcgtgggcgc ctgcctgcac gtgacggccg agacggccaa
    13081 cctcgtgcag gtgctcacgg cgggtggggc ggaggtggcg ctgtgcgccg ccaacccgct
    13141 ctccacccag ccggacgtgg cggcggtgct ggagggcgac ggcgtggagg tccacgccga
    13201 tccggcctcg tacgagcgca acatcgacgc ggtcctcgcg cgcgggccgc gcgtgacgat
    13261 ggacgacggc gccgacctcg cgctgcgggt cgagggcgac gtgctcggcg ggacggagga
    13321 gacctccacg ggagcgctgc ggctacgcgc ggccaaggac ctggcgttcc cggtcatcgc
    13381 cgtgcacgag gggcgcaccg cgcgcctgtt cgacacccgc tacggcaccg gccagtccac
    13441 gctcgacggc atcctgcgcg ccaccaacgt gctgctcgcc ggccgcaccg tggtggtggt
    13501 cggcttcggc ggctgcgggc gcggcgtggc gctgcgagca aaggggctcg gcgcgcacgt
    13561 gatcgtgtgc gaggtggacc cgctggcggc gctcgaggcc gccatggagg gctaccgcgt
    13621 gatgccggcc gccgcggcgg ccgccgaggg cgacgtgttc atcacggcca cgggcacgcg
    13681 tgacgcgctg acccgcgagc acttcgaggc catgaaggac ggcgcgatcc tcgccaacgc
    13741 cgggcacttc gacgtggaga tcgacaagcc ggcgctggcc gagctgagcg gcgcggtgcg
    13801 cgaggtgcgc ccgctggtgg ccgagcacgt gctggccggc gaccgccggc tctacctgct
    13861 cgccgagggc cgcgtggtca acctcgcgtc agccgagggc cacccggccg cggtgatgga
    13921 cgtgggcttc gcgacacagg cgctggcggt cgagcgcatc gtccgcggcg acctcgcgcc
    13981 gggcgtgcac ggcgtgcccg acgagatcga ccgcgaggtc gcgcgcctgg agctggcggc
    14041 cctgggcgtg cgcatcgacg agctgaccga cgcccagcgg gagtacctgc acagctgggg
    14101 ctagggcgcg agtccttttc ttacgcctat agccgtaaga aatggactct cgccccgctg
    14161 gtagcctcgc gcgccctatg aaggccatgg tcctcgccgc cgggctcggc actcgcctgc
    14221 gcccgctcac ctacgagatc ccgaagccga tggtgccggt cctcgaccgc cccgtgatgg
    14281 ctcacatcct cggcggcctc cggcgtcaag gcttcgacga ggtgatcgcg aacctccact
    14341 acttcccgga cgccgtgaag ggctacttcg gcgaccgggt cgagtaccgc tacgaggagg
    14401 agctgctcgg caccgccggc ggggtccgca acgtggccga cttcttcggc gacgacccgg
    14461 tcgtcgtcat ctccggcgac gccctcaccg acgtggacct gaacgctctg gtcgagcgcc
    14521 accgccaggc cggcgggatc ggcacgctca ccgtcaagaa ggtgccggac acgcgtgagt
    14581 acggcgtggt tatccacgac tccgagggcc ggatccaggg cttccaggag aagccggagc
    14641 ccgccgaggc gctctcggac ctcggcaact gcgggatcta ctgcttcagc ccggagatct
    14701 tcgactactt ccccgaggag ccgttcgccg actgggcgca ggacgtcttc ccggcgctgc
    14761 tcgagaacga cgtcccgttc cacgcctacg agaccgagga ctactggaac gacgtcggct
    14821 cgctcgccga gctgcgcgag ggcacctggg acgcgctcgc ggggcggctc aagatcgagg
    14881 tcaccggcgc gaccgacggg gacccaccgg tctggaccgg tcacgacgtg gacatcggct
    14941 ccgacgtgcg cctgatgggc ccggtcgcga tcggcgaccg ctgccggatc ggcgacggct
    15001 gcgcgctgcg cgacacgatc gtcttcccgg gcaccgaggt ggcgcccggc acgatcgcga
    15061 tcggcgcgat catcggccgt ggcgggatcg tcgaggccat gcggccgctc gacgagctcg
    15121 aatagccccc agaacgacga aaccccgcct tccctagcgg ggcccatcgg ccgcatccct
    15181 gcgacccgtc cgtgcgcgca gtcctgcgct taggcgcgct tggcgcctcc acccttcaca
    15241 acgagctagg cgggccgagg ttgcgcagtt ggcggcgcca actcgcgcac gagttcggcc
    15301 gctgaccgca cgtcgccgat gcgcgccacg gtctcgcccg cgtacagggg gccggactcg
    15361 agcaggttct ccccgcggtc gtcggtgggc gcatacgggc tcagcagcgg gatcgcggga
    15421 cgctgtgccg ctatcagccg ctcctggagc cccgtgggcg cggccggcat gagccgcccg
    15481 gtcaggcgat tgagcgcacc gatcggccgc tcgcgccgtc gccagcgccg caccgcctcg
    15541 ttctccacca cgcggtgggg agcgtccggc cagcccatcc cgaacagctc ggtgagcacc
    15601 gtctcgcgcg cctccacgag ccgctcgcgg taagcggggt gggcgccgct ctcgggcgtc
    15661 atcacgaagc gggtgccgag cacggcggcg tcggcgcctg cgtcgagggc ggtgcgcacg
    15721 tcgtcggcgg tcgcgatccc gcccgcgagc agcagtgggt agggcgccgg cagctccgcg
    15781 cgcgcctgcg cgagcagctc gagcgcgggc agcttgccgc gcacgtggcc gcccgcctcg
    15841 cggccctgca cgatcacgcc gtcggcgccc gcctcccggg ccgcccgggc ctccgcgagg
    15901 ctcccggcct gatgcagcca tacccgcgcc gagcccggcc gccgcggctc gccccagaac
    15961 gtgaccaccg cgtcggcctc cgccgcggcc tcggcgtgcg cacggcccgc gaacggcagc
    16021 aggaggtttg ccgcgaccgg cctctccgtc tgctcgcgtg ccgccgcgat ctcgcggcgc
    16081 agctcgcccg gcggcaggaa cccgatcgtc ccgagcccgc ccgcgccgga gactgccgcc
    16141 gccagccgcg cgcgcgagat gcccccgccc atcccggcct gcacgaccgg gcgctccacg
    16201 ccgaggcggt cgagcagctc gctcacgccg ccgccgcgtc ctcgagccgc tcgcgccggc
    16261 gctcctcctc ctcggactgg acgagcatcc gcccgaacag gaagaccagc acgatcacga
    16321 ggacgatcga ctgctcgacc atcatcatcg ccccgcccac gttctggtcc tcgatcggcg
    16381 tgaggcccca gatgcgcggc accgttccgt agtggtcgta gagcaggttg ctcgaccagg
    16441 tcaggtagat gccgagcgcg gccaggccga tcttggcgct gaagatgtag gcgaacggcg
    16501 cgatgccggt caggcggcgg cgcatcggga ccggctggat cagcggccac cagacgagcg
    16561 tgccggcggt gaagaacgtc atgtgctcga gggcgtgcac cggcgcgtgg tggatggctc
    16621 cgacgtacag cgcgggtatg tgccaggtgt agaccagcgc gagccagagc aggatgaacg
    16681 cgggcgggct ggcgagcgcg ccgagccggc gctcgacgcc gtgcaggcgc cgcgtggccg
    16741 ggcgcatgat cacgcgcgag aggccgagca gcaggagcag gggcgccaag tcgccgagca
    16801 ggatgtgctg ggccatgtgg gcgacgaaca ggtagtcctc gccgaggcgg tcgagcggtg
    16861 agatcagggc cgcgaagagg atcagcatgg agaggccgaa gcaggtggcc tggagcggtc
    16921 cggcgccgcg tccgccggcc tcgcgccgcg ccgcgcggaa gcggcgggca tagaggaaga
    16981 tgccgaccgc gagcgcggcc agcgggccga tctcgaggct ccagtggagt tgtgggtgcg
    17041 aggcggcgct cacctgtcac ccaatcgttg cagccgtgac cctcctgtaa cgcggcagcc
    17101 gcgaagctgg gcccgtggcc ctcgctcggc tgatcggatt gctggcaccg cgactgtgcg
    17161 cctcctgcgg cgcggtggcc ggcgacgccg agccgctgtg cggtcgctgc cgccacgcgc
    17221 tggcgtggct cggcccggcc gcggtcgagc tcgcgggggt gcgcgtgtgg gcgccggtct
    17281 cctacgccgg gcccgcccgc gacctggtcc gcgccctgaa gttcggcgga gcctggcgcg
    17341 cggcggagac gatggcggcg cagatcgccg cgaacgcgcc gccggagctg ctcggtgacc
    17401 gagctggtcg atctaatgcc cgctatacgg gcctaggatc gaccagttcc ccggcggagg
    17461 gccggccgga gctactgccg ggcccggcgc acgcggcgcg cgcggagggg cccgcggcgc
    17521 tcgtccccgt cccgctccac ccccgccgcc ggcgccggcg cggctacgac caggccgcgc
    17581 tgctcgcgcg ggccctcgcc gagcgcacgg gccttcccgt ccgcgcgtgc ctcgaccgca
    17641 gcggcgccgc cggcgcccag gtcggccgcg ggcgcgccga gcggctcggc gggccgcccg
    17701 gcgagatccg gctccgcccc ggcgcggaag ccccgcgcgc cgcgcttctg gtcgatgacg
    17761 tcgtgaccac cggcggcacg ctcgcctcct gcgccgccgc gctccgcgcc gccggcaccg
    17821 cggacctcgc agcgctggcg tatgcgcgca cgccgggccg gtaggccgct tgcgccccac
    17881 ccgcatcgcc gataggttcg atcccgatgc ggctcgccac gatcaccgcc ctcgcggcgg
    17941 gcctggccct cgcgctgccc gcgtccgggc aggcgaagaa cctctgcgtc ggcgcgcccg
    18001 gcggcgcctg cgactcctcc cacgcctcga tccaggacgc gatgaacgtc gcgtcggacg
    18061 cgggccaccc cggccgcgac acgatccgga tcggcaaggg cctgttcgag gcgcctccca
    18121 tctcccagcc gaacgcggcg cagaaccccg tcgacgtggt cggcagcggc caggacatcg
    18181 acggcacccg cctgcgctcc accacggccc agcccgcgat caggctgacc acggccagca
    18241 cgatcgacaa cgtgcgcatc ctgatcccgc cggccggagt gggcgggctc gacctcgagg
    18301 cggccgacgc ccggaacgtg accgtgttcg cggagccggg cgacggcgcc gtgaccggca
    18361 agacgggcgt ctacatgcgc aaggactcgt cgctgcgcaa ctcgacagtg agcctcgtca
    18421 ccgcgcccgc gcccagctac gccgtctcga tcaccgatgc cggcaaccac gtgatcgagg
    18481 acgccctgct cgacggcacc gacctcgtcg acctgaccgg gtacgccacg caggccacga
    18541 tccgccgcat ccgcaacagc ggccgctgct cgagctgcct gtacctgcgc ccggccgcgc
    18601 cggacaagac cgtcgacatc tccgacagcc tccttcgcgt cgcggccgag ggccagggcc
    18661 tctacgcgca gcccggcacc acgatcaacg cccgcaacaa cacgatcgtc ggagtgcccg
    18721 gcacgaacac ggccggcgcc cgcgccaacg gctccgggca ggtcaacgtg ctcgactcga
    18781 tcatccgcga cgtcaactac gccctcttca ccgacggcag cgcccacctg tcgatcgact
    18841 actcggactt cgacccggac aagctccaga aggtcggcgt cgccggcctg ttctcgatcg
    18901 gcgcgcacaa cgtgaacgcc gacccgaagt tcatgaacgc cgacgtcggc gactaccgcc
    18961 tggcgagcgg ctcgccggtc gccgagcgcg cgtcggccgg ggctggcgcc ggcgacctgg
    19021 acctcgagcg caagccgcgc ctcgcggacg ccgacggcga cggcaccccg atccgcgaca
    19081 tgggcgccta cgagtactcc ggcgcgccct acgcgctccc gccggtcgag tacatcccgc
    19141 acggcgacgg tggcggcggt ggcggcggca cgatccaggg cccggtcatg accttcaagt
    19201 cgaagaccgt caagctcgac cgcaagcggc gcgtgaaggt cgggctcacc tgcccgcaca
    19261 gcgagatcgg cttgtgcagc ggccgggtac ggctcgtcag cacgaagaag atccgcccga
    19321 agccgggcaa gaaggccgtc tacgtcggcg tcggcgccgc ggacttcaaa tcgatcccgg
    19381 ccgacaccac gcgcacggtc acgatcaagg tctcgaagaa gaccgcggcg atcctgcggc
    19441 ggttcaagtc ggtgaaggtc acgggcacga tcgtggccag ctggcagacc ggcgacccgc
    19501 gcaccagcac ctacccgctc accctgaagc ccagcaccaa gtagtcggcg tagcatcccc
    19561 cgggtcagcg accggagagg agctcgcatg cggatcgagg tcaaggggcg caacggggtc
    19621 gtggtggacg acgaccttcg agccaggatc gagaagcgct tcgagaaggt cgcacggcag
    19681 gtctccgagc tggccgtcct ggaggtcgag ctgtcggagg agaagaaccc ctcgatcaag
    19741 gaccgccagg tggccgaggc gacgctgcgg ctgaagggca cgacgctccg cgcgcgcgac
    19801 gcctccgagg acctctccca cgcgatccac ctcacctgcg acgacctcgc ccgccaggtg
    19861 aagaagcacc gcgacaagcg gcgcgggcgc cgccatgccg caagcgccga agcacctccg
    19921 gccgctccgt agccaagcgc taacctcgac gcgatgtcga tcctcgaccg cgccctccgc
    19981 atcggcgagt ccaagaagat caagggcttc cagcagaggg tcgcccgcat caacgacttc
    20041 gaggccgagc tcgcgctcga cgacgaccac gagctgctcg aacgcgccag ctccctgcgc
    20101 gagcgcgccc gcaacggcga ggacctcgac cggctgctcc ccgagtcgtt cgccctgacc
    20161 cgcgaggccg cgaagcgcgc cctcggccag cgccacttcg acgtgcagct gatcggcggc
    20221 atggtcctgc acgacggctc gatcgccgag atgcggaccg gcgagggcaa gaccctcacg
    20281 gccacgctgc cggtggtcct gaactcgctg cccggcaagg gcgtccacct cgtcacggtg
    20341 aacgactacc tcgcacgccg cgacgccgag tggatgcgtc ccgtctacga gatgctcggc
    20401 gtgagcgtgg gcgtggtcca gtccgacgag cgcgacgcgg ccaagcacgc cgcctacgcc
    20461 tcggacgtca cctacggcac gaactccgag ttcggcttcg actacctgcg tgacaacctc
    20521 gccacctcgc tcgaggacca gtaccagcgc ggcgtccaca acttcgcgat cgtcgacgag
    20581 gtcgacaaca tcctcatcga cgaggcccgc accccgctca tcatctccgg acagccggag
    20641 caggcggccg acgtctatta caagttcgcg gccctggcca agcgcatggt ccccggcaag
    20701 aagcccgagg gcatggaggc caagtcgaag gagtacgagg ccgacttcga cttcgagccc
    20761 gacgagaagc acaagaccgt cgcggtcacc gagcgcggcg tggcgaaggc cgagaagttc
    20821 atggggatcg agaacctcta cctcgccgag cacggcaacc tcgtcaacca cctgatccag
    20881 tcgctcaagg ccgaggcgct ctacaagcgc gacgtcgact acgcggtgat cgacggcgag
    20941 gtcaagatca tcgacgagtt caccggccgc atcctcgagg ggcggcgctg gtccgagggg
    21001 ctccaccagg cggtcgaggc gaaggagggc gtcccgatcc aggaggagaa ccagacggtc
    21061 gcgacgatca cctaccagaa ctacttccgc cgctacgaca agctcggcgg catgaccggc
    21121 acggccctca ccgaggccac cgagttcatg aagatctacg agctccaggt ggtcgaggtc
    21181 cccaccaacc gcccgatggt ccgcaaggac cagaacgacc agatctacaa gaccaaggac
    21241 ggcaagtggg cggcggtcgt gaacgagatc gaggcgcgcc acgagaagga ccagccggtg
    21301 ctggtcggca cgatctcggt cgaggtctcg gagctcctgt cgaagcagct cgaggatcgc
    21361 gggatagagc acgcggtgct gaacgcgaag cccgagcacg ccgagcgcga gggcgagatc
    21421 atcgccgagg ccggccgcct cggcgccgtc acgatcgcga cgaacatggc cggccgcggc
    21481 gtcgacatca agctcggcgg caacgccgag caccagaccc accaggagct gctgaagctc
    21541 ggcctcgatc ccgactcgaa cgactgggac gaggagtgga acgcgctcta cccgcgcatc
    21601 gaggcgaagg tcgaggagga ccgcgagaag gtcaaggagg tcggcggcct ctacatcctc
    21661 ggcaccgagc gccacgagtc gcgccgcatc gacaaccagc tccgcggccg tgccggccgc
    21721 cagggcgacc cgggcgagtc gcgcttctac ctctcggccg aggacgacct cgtgcgcctg
    21781 ttcgcgggcg accggatcta ccggatcctc gaccgcctcg gaccggtcgg cgaggagggc
    21841 gaggagcaac cgatcgaggc cgggatgctc tcgaagcaga tcgagaacgc ccagaagaag
    21901 gtcgaggagc agaacttcct gatccgcaag cgcgtcctcg agtacgacga cgttatgaac
    21961 cagcagcgcg agatcatcta cgagatccgc cagcggatcc tcaagggcga ggacatgtcg
    22021 gagaccgcgc gcacgcagat cgccgacgcc atcgagcgca tgaccgacga gtacctggtc
    22081 ggcgacttcg tcgaggactg ggacgtcgac gcgctgctca cgcagatcga gcagatctat
    22141 ccggtcgcga tcgcgcccga cgacctcgac ccgaacacga tcgagcgcag cgcgctgatc
    22201 gccgagctgc gcgaggacgt ggtcaaggca tacgacgagc gcgaggagga gctgggctcc
    22261 gacctgatgc gccagctcga gcgcttcatc ctgctccaga tcatcgacga ccgctggcgg
    22321 gagcacctgc acgacatgga ctacctgcgc gaggggatcc acctgcgcgg gttcgcccag
    22381 gaggagccgc tcgtcgcgta caagaacgag ggcttcggga tgttcaccac gctggtgaac
    22441 tcgatctggg aggagttcgg gcgctacatc ttcaacgtcg aggtcgagat cgagggcgag
    22501 gaggcccagg ggcaggcgtc gaactggcag ccctcggcca gctcgagctc gaccggcaac
    22561 ctccagtacc agggcggccg gcccgaggac cagccgagcg cgctccaggc ggccgccgcc
    22621 ggcgcgctcc cggtcgccgc ggccgacgcc gcggtctcgc ccgactccaa cggcgcgccg
    22681 gaggaggccg ctgcggcggc gagcgtcgag acgcgccgcc tcggcgagca cgagcagatc
    22741 ggccgcaacg acccgtgctg gtgcgggtcc ggcaagaagt tcaagaagtg tcacggggcc
    22801 tagcgccatg agcgccaccg cgcccctccg cgaccagctc aagctgctcg cggactacct
    22861 ttgacccagc cgctctagcc gaacgcctcg gcacgctcga gcaggagatg ggcgagcagg
    22921 gcttctggga cgaccaggag cgcgccgccc gcgtgtccgc cgagcactcg cgcgcgcagc
    22981 gcaagctcga ccgctaccgc tcgctcgagt cgcagatcga cgacctcgag accctcgagg
    23041 agctggcggc cgacgacagc tcgatcgccg aggagctcgc cggccagcgc gtcgaggtcg
    23101 aggcgctgct cgccgacctc gaggaggagc ggctgttcgc cgggcgctac gactcgggcg
    23161 acgcggtggt gtcggtccac gcgggcgcgg gcggcaccga ctcgcaggac tgggccgaga
    23221 tgctgctgcg catggagatg cgctgggccg agcggcgcgg gttcggcgcc gagctgaagg
    23281 aggtgagccc gggggaggag gccggcatca agtcggcgac cttcgtggtg aagggggaga
    23341 acgcgtacgg gctctactcg gcggagaagg gcgtgcaccg gctggtgcgg atctcgccgt
    23401 tcgacgcggc gtcgcggcgt cacacggcgt tcgcgctggt ggaggtgtcg ccgctcgtgg
    23461 agggcagcgt cgacgtggag atcgacgaca ccgacctcca gatcgacacc taccgcgcct
    23521 cgggcgccgg cgggcagcac gtgaacaaga ccgactcggc ggtgcggatc acgcaccggc
    23581 cgtccgggat cgtcgtgcag tgccagaacg agcgctcgca gacctcgaac aaggcgacgg
    23641 cgatggcgat gctgcgctcg aagctgctcg agcgcgagga gcagcgccgc cgcgaggaga
    23701 tggcgaagga gaagggcgag tcgcaggacg tggcgtgggg ctcgcagatc cgctcgtacg
    23761 tcctgcaccc gtacacgatg gtcaaggacc accggacgag ccacgagatg ggcgacgcca
    23821 gccgggtgct cgacggcgac ctcgacggct tcgtgcgcgc ggagctgctg cggcgagcgg
    23881 cggcgtagac gtgcaggagc tgatcgaccg cgcgctggcc gaggacgtgg ggtccggcga
    23941 cctgaccacg gaggcgctcg tcgcgccgga gtcgcgctcg gccgcccgga tcgagcagaa
    24001 ggcgcccggc gtcgtgtcgg gcctgcgggt ggcggagtcg gtgttccggc gcgtggaccc
    24061 ggagctgcgc tggcacggcc acgcggccga gggcgagtgg cgcgacggcg ggctcgtggc
    24121 ggagatcgcg ggctcgaccg cgtcgatcct gcgcggggag cgcgtggcgc tgaacttcct
    24181 cgggcggctg tcgggcgtgg cgacgctcac cgcgcgctac gtgcacgcgg tcgaggggac
    24241 cggcgcccgg atcctcgaca cccgcaagac cacgcccggg ctgcgcgcgc tggagaaggc
    24301 tgccgtggcc gacggcggcg gcgtgaacca ccgcttcggg ctcttcgacg cgatgctcgt
    24361 gaaggagaac cacatcgcgg cggcgggggg cgtcggcgcc gcgacccgct tggcgcttac
    24421 ggcggctggg gagggggtgc ccgtggagat cgaatgcgct tcgctcgccg aggtgcgcga
    24481 ggcgctcgag gccggcgcgc cccggatcct cctcgacaac atgacgctcg acgagctgcg
    24541 cgaggccgtg gcgctgacgg agggccgggc ggagctggag gcctcggggg gcgtcacact
    24601 ggagacggtg cgtgcgatag cggagaccgg cgtcgacttc ataagcgtcg gagccctgac
    24661 ccacagcgcc ccggccctcg acctctccct cctcctggac cccctttgat cgcaaccttc
    24721 ctgccctgga gggacagtgt ccgcctaatc caggaaagcc gacggaaagc gctggtactc
    24781 gccgccctcc tcattgccgc cgttctcgcg tcggccccgc cagcctcggc ccagtcggat
    24841 cccctgagcg cgctgcgcac gtcctgcgcc gccaagaagt cccccgacgg cgcgcgctac
    24901 cgcatctgca cggcccaggt cgcgagcttc gacggcaccc cgctcgacgt gaccctcacg
    24961 ctcccggcca agcgcagcaa gcggcgcgtc ccgctcgtcg ccttcctcca cggcttcctc
    25021 agccagaagg gcgagtacct gagcgagacg cgcgagggca ccgggcccga tcgcggcgcg
    25081 gacgcctaca agaccgtccg ctggaacaac gtctggttcg cctcgcgggg atacgcggtg
    25141 ctgaactaca cggcgcgcgg gcacggcgat tcgggcgggc agatcggcct cgcgagccgc
    25201 gacgtcgagg tgcgcgacac gcaatacctc accggcctgc tggtggaccg cgccgggccg
    25261 ctggcgcgga tcgaccggcg cagggtcgga gtgatcggag ggagctacgg cggcggccag
    25321 acctggctgc tgatgaccac gcggccatcc gcctcgctgc cgtatggcac ctggctctcg
    25381 ccggcggggc ggcgggtcga gctggcggcg gtcgtgccca tgtacacgtg gagcgacctg
    25441 ctctacgcgc tcgtgccgaa cggcaggcac ctgtcgagcg gcgtggaccc gcgcacggcg
    25501 gcgagcccga tcggcatcgc caagctcacg ctgctcgacg gctttctcgc caccgccggc
    25561 ccgcggctgc cgcagcaggt atacggctgg ctcgcgcgcg tgaacgccgg cgagccgtac
    25621 gaggggggcg acccgctgct cgaggaggcg cgccgggagc tgactgtcac gcgctcgggc
    25681 ttctaccagg acggctactt cgaggccctg cgccgcagga aggtgcgccg cgtgccggta
    25741 ctcgccgccc agggctggac cgacccgctg tttcccgcga tcgaggtgct gcgcatgtac
    25801 cggcgcctca aggcgtcgag ccccggttac ccgatccagc tctacctcgg ggacttcgag
    25861 cacctcacag cgcgctcgaa ggtcgccgac ctgcgccact tccacgtgct cggcaaccgg
    25921 ctgctcgacc gccacctgcg cggacgcggg cccaagccgg tgctggacgc gcgggcggcg
    25981 gtcacgaact gcgacgagaa gcgcttcggg ccggtgctga aggcgaagac ctgggacggg
    26041 ctggccggcc cggcgctcag cttcgacctc ggcgggccga agcagaccac ggcgcgcgcg
    26101 gcggggcagg gcccggccac cgatcccatc gtgctcgcac agcagcgggg gaggggctgc
    26161 atcaccacga cagcggcaga tagccccggt tcggcccgct acagcattcc cctcactgcc
    26221 ccggcgacgc tgctcggcct gccgcgcgtc acggtgacct acaccgccac cgcacccgac
    26281 ttcgagctga acgcccacct ctgggacgtc gcccccgacg gcaccaggac cctgatcgac
    26341 cgcggcgcct accgcggcgg gccggtgctg agcgggacgg ccgactacga gctgttcggc
    26401 aacgcctggc gcgtggaagc cggccaccag ctcgagctcg agctgctcca ggacgactcg
    26461 accttcctgc gcccgcacaa ctacccgtcg accgtcacag ttcagaacgc gcgtttggga
    26521 ctacacttgg ctgcgcccgg ctaggcccgc gggcttcatt tcgacacctc gaaggaggct
    26581 cgaaacatgc agctgccaac ggcgccctcg ggcgcgacta tcgacgcacc gctggcgctc
    26641 gaggacatcc ctgcgctcca gcgcgaggtt cgcgcgctcg ccgagcagcg caacgcggtg
    26701 atcctcgccc acaactacca ggtgcccgag gtgcaggacg tggccgactt cgtcggcgac
    26761 tcgctcgccc tctcgcgcaa ggccgcggcc gccggggcgg acacgatcgc gttctgcggc
    26821 gttcacttca tggccgagac ggcctcgatc ctgagcccgg acaagaccgt gctgctgccc
    26881 gacctcggtg ccggctgctc gctcgccgac tcgatcaccg ccgaccagct ccgcacctgg
    26941 aaggcccagc acccgggcgc gatcgtcgtg atgtacgtga acacgaccgc cgaggtgaag
    27001 gccgagaccg actactgctg cacgtcctcg aacgcggtgc aggtggtcga gcacatccgc
    27061 cgcgagcacg gggaggacgt cgagatcctg ttcgggcctg acatgttcct cggcgcctac
    27121 gtcgagcgcg tgaccggcag gccgatgcag gtgtgggccg gcgagtgcca cgtccacgcg
    27181 ggcatccggc cgtcggacat cgcgaaggtg cgcgcggaga accccggtgc ggagttcctg
    27241 atccaccccg agtgcggctg ctcgacgtcg gtgatggagt acgtcgccgc cggcgacgtc
    27301 gacgccgaga acacgcacat gctctcgacg ggcgggatgc tcgagcacgc tcgcagctcg
    27361 gacgccgaca cgttcatcgt cgcgaccgag acggggatgc tgcacccgct cgagcaggag
    27421 aacccgggca agcggttcgt ggcggcgaac agctgtgccg tctgcccgtt catgaagatg
    27481 atcacgctgc cgaagctgcg cgacgcgctg cgcgacggcg gcccgcggta caccgtgaag
    27541 gtcgacgccg agctcgccga gcgagcgcgg gtgccgatcg agcggatggt cgcgatcggc
    27601 ggctgacctc cgctgtgtcg ccgggcgggc accgctcgcc ggcttgacac gaaagaaggg
    27661 ggcggccgaa gccgccccct tcgattgccc ggccggcgag gggccgccgg cgggcgctcc
    27721 cgcagggcct agggcttgag gagctcgacc tcctcgaaca cggccttgcc gtcacgcagc
    27781 tcgacctcgg cctcgtggac caccgtgccc ggtgtcaggt cggcggtggt gcacacgcgg
    27841 tcgtcgtcgt cgcgatcgtc gtcgttgcga tcgtcatcgc gagcgtcgtc gtcgccacgg
    27901 tcgtcgtcgc cacggtcgga cgtggtgctg ccgggcccac tgttggagct cggcccgctg
    27961 ttgtcgtcgc tgccgtcccc gtcacggccg gggcccgagc tggcgctggt gacgccgctg
    28021 cggtcctcgc actcgatgcg cgtcgtcgag tcgacgagcc cggtgagcgt gccgccgccc
    28081 gagacgttga tcgtgagcgt gcccgtcgcc gag
//
LOCUS       DASVPL010000275         5572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_188868, whole genome shotgun sequence.
ACCESSION   DASVPL010000275 DASVPL010000000
VERSION     DASVPL010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188868"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..215)
                     /gene="rpmF"
                     /locus_tag="VF517_15365"
     CDS             complement(21..215)
                     /gene="rpmF"
                     /locus_tag="VF517_15365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX8054366.1"
                     /translation="MAVPKQKQSHSRTNKRRSTHKITPPSLRSCPQCHTPRLPHRVCP
                     VCGHYKGEQVYTPGSTDLAE"
     gene            complement(249..2540)
                     /locus_tag="VF517_15370"
     CDS             complement(249..2540)
                     /locus_tag="VF517_15370"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8054367.1"
                     /translation="MSLARRTLLPLAIVLAAFAVSAATAAAAGNLAQLPGQLGCISES
                     GTGGSCFDGAGLVAAKAVATSPDGRHVYVFSNASATIQTFTRDEATGALTRVPGADGC
                     VSDPGTEGCRAVRGLTAGFDIAVSPDGRSVYTAAFSDSAIAVFARDPGTGRLTQPDGA
                     AGCVASTVADGCTDARAVNGIRSVAVSPDGRTVYGAASVDDGVAVFTRNPDTGVLTQP
                     AGAAGCLVENPATGDNAGCTDGRALDTTHGVDVSPDGHQVYASANGIEAVSTLDRNTD
                     NGTISQSIETASCVSETGAGPCLDGSALTNPQTVLASPDGRHVYAAGASADSVVTLVR
                     DAGSGRLTPVAGATGCVSETGSGCENGVALQNPNGLAISPSGQTVYVASLNSDSVVRL
                     DRNAETGELNQPAAPAGCISEDGTGGACGDGVALDSPDDVAVAPDGANVYAVSSTSDA
                     VVAFARGDRPVCSDVAASTAYVTAVTFQLPCADPNGEPLSFAVLSGPAVAGLGLVEAT
                     GAATYTAPAGFFGTVSFTYRVTAEDGRSGVATATVSIAGPTAPCTLVFTGTAAGETLL
                     GTDFGDVIGGLAGGDWIEGLGGDDCLDGGDGNDRLFGDDGADTLRGRGGRDALDGGAG
                     NDVLSGAGDRDKLSGRAGDDTLTGSSGNDSLRGWEGNDKLGGGSGKDVLDGAAGKDTL
                     NGDSGNDVLTGGDGNDKLNGSSGNDRLTGGRGKNAYSAGSGNDDVRANNRRNEKIDCG
                     RGRDRVRADRGDKLKGCEIVVRV"
     gene            complement(2582..3100)
                     /locus_tag="VF517_15375"
     CDS             complement(2582..3100)
                     /locus_tag="VF517_15375"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX8054368.1"
                     /translation="MSAQTDVFELGRLQLSSGSARSIDLQVKLDTFEFGAQQYQVADD
                     LVDVRLDAARTVSGYSLRLRFEAELDGPCMRCLDAAAPLVDVDAREIDQPGEDDEELS
                     SPYVTGDELDLQAWARDALALALPTQIVCGPDCRGLCPVCGENLNRADPGHAHEAEPN
                     PRWAKLSELRFE"
     gene            3147..3758
                     /locus_tag="VF517_15380"
     CDS             3147..3758
                     /locus_tag="VF517_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8054369.1"
                     /translation="MRLYRAPFSTNSERVALALAHKGIEVESVWIDYGDRGAVEAVSG
                     QGLVPVVEDGGEVVFDSPRILAWLEERRPDPPLFPASDPRRAELEVFVDWFNAVWKPW
                     PNGIEAELGKVLPDQELIDAHVASMADALDQFERLLAGRDHLMGDEFSVADCVVFPFV
                     KFALRRDPEDDELFHRVLDDYQSVDGRPRLEAWIERVDARPRA"
     gene            3755..4249
                     /locus_tag="VF517_15385"
     CDS             3755..4249
                     /locus_tag="VF517_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054370.1"
                     /translation="MRRALACLVAVAALAGCGGGGDGGGSTKSEQPAERTGAITKDPA
                     AERVLRDASDAFTKGRADMLASLQTDLRQNDTQTLVDDLWDLRNVIYKFDQALRRIEF
                     APGRAERVSVEILEIDRGAIARIDPILDAKEPPPGLADAVQQAATDAAAIERKTRDLI
                     AGTG"
     gene            complement(4246..4707)
                     /locus_tag="VF517_15390"
     CDS             complement(4246..4707)
                     /locus_tag="VF517_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054371.1"
                     /translation="MDVLVLIDKLDDTVHNAKPVPLTDQVRVDKEEIYDILDQMRATI
                     PEEIKQARWIVKERQEMLAEAKREAERILKESREEQARLIASEEVVRQAERQAEEIIE
                     EARTREREIRLGAEDYADDILNTLEVNLQKFTAAVQRGRDRLAGREEAEVG"
     gene            complement(4715..5374)
                     /locus_tag="VF517_15395"
     CDS             complement(4715..5374)
                     /locus_tag="VF517_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054372.1"
                     /translation="MSLHQLVDQLEAMIRDAKETPILGGARIDRESVYDLVDQMRASI
                     EFVLLDEDHGTYRGAGALVPLDELDTFMYDAKSVPLTGSVRVDRDRLLAIVGRVRQGA
                     AQDRYVTREEFERTAVDPRRARTAFDRLDTLVRKARPVPLTTQVRLDKGAVAKVVEEL
                     RGWGPVALLPAIDALDDLLHNAHPVPLTNEVRVERATLSEIYQQMRAGFSQGTPPALT
                     N"
     gene            complement(5379..>5572)
                     /locus_tag="VF517_15400"
     CDS             complement(5379..>5572)
                     /locus_tag="VF517_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054373.1"
                     /translation="YEFEMNQLNRKMAPDIESIYMFSSPQFTFLSSSGVKELAMFGGD
                     LSGLVPDSVLPRLEEALRR"
BASE COUNT          703 a         1987 c         2043 g          839 t
ORIGIN      
        1 gcccccgggc ccggtcaccg ctactcggct aggtcggtgc tgcccggcgt gtagacctgc
       61 tcgcccttgt agtgcccgca gacggggcag acgcggtgcg gcagccgggg cgtgtggcac
      121 tgcgggcagc tgcgcaggct cggcggcgtg atcttgtggg tcgaccggcg cttgttcgtg
      181 cgcgagtgcg actgcttttg cttggggacc gccatcgggc ggccagtcta gcggcgcccg
      241 gagggcccct agacccggac gacgatctcg cagcccttca gcttgtcgcc cctgtcggcc
      301 cgcaccctgt cgcgcccgcg gccgcagtcg atcttctcgt tgcgcctgtt gttcgcgcgc
      361 acgtcgtcgt tgccggagcc cgcggagtag gcgttcttcc ccctgccgcc ggtcaggcgg
      421 tcgttgcccg aggagccgtt cagcttgtcg ttgccgtcgc cgccggtcag cacgtcgttc
      481 cccgagtccc cgttcagcgt gtccttgccc gcggcgccgt ccagcacgtc cttcccgctg
      541 ccgccgccga gcttgtcgtt gccctcccag ccgcgcagcg agtcgttccc cgaggagccc
      601 gtgagcgtgt cgtcgccggc ccggccggag agcttgtcgc ggtcgcccgc gccgctcagc
      661 acgtcgttgc cggcaccgcc gtcgagcgcg tcgcgcccgc cgcggccgcg gagcgtgtcg
      721 gccccgtcgt cgccgaacag gcggtcgttg ccgtcgccgc cgtcgaggca gtcgtcgccg
      781 ccgaggccct cgatccagtc gccgccggcc aggccgccga tcacatcgcc gaagtcggtg
      841 ccgaggagcg tctcgccggc ggcggtgccg gtgaagacga gggtgcacgg ggcggtcggg
      901 ccggcgatgg agaccgtcgc ggtcgcgacg ccggagcggc cgtcctccgc cgtcacgcgg
      961 taggtgaagc tgacggtgcc gaagaagccg gccggggccg tgtacgtggc cgctcccgtg
     1021 gcctcgacca gcccgagccc ggccaccgcc ggaccggaga gaaccgcgaa gctgaggggt
     1081 tcaccgttcg ggtcggcaca cgggagctgg aacgtcaccg ccgtgacgta cgcggtcgac
     1141 gccgccacgt cgctgcagac cggccggtcc ccgcgagcga aggcgaccac cgcgtcggag
     1201 gtcgaggaga ccgcatagac gttcgcgccg tcgggcgcga ccgccacgtc gtcgggcgag
     1261 tcgagcgcga cgccgtctcc acacgccccg ccggtgccgt cctcgctgat gcacccggcg
     1321 ggcgccgcgg gctggttcag ctccccggtc tcggcgttcc ggtcgagccg gaccacggag
     1381 tcgctgttga ggctcgccac gtagacggtc tgcccactgg ggctgatcgc aaggccgttc
     1441 gggttctgga gcgcgacacc gttctcgcac cccgatcccg tctcgctgac gcagccggtg
     1501 gcgcccgcaa ccggggtcag ccggccgctc cccgcgtcgc gcacgagcgt gacgaccgag
     1561 tccgcggacg cgcccgccgc gtacacgtgg cgcccgtccg ggctggcgag cacggtctgc
     1621 gggttggtca gggcgctgcc gtcgaggcac gggccggcgc cggtctcgct gacgcacgag
     1681 gcggtctcga tggactggga gatcgtgccg ttgtcggtgt tgcggtcgag cgtcgagacc
     1741 gcctcgatcc cgttcgccga cgcgtacacc tggtgaccgt ccgggctcac gtccaccccg
     1801 tgggtggtgt cgagggcgcg gccgtcggtg cagccggcgt tgtcgccggt ggccgggttc
     1861 tcgaccaggc acccggccgc gccggctggc tgggtgagca cgccggtgtc cgggttgcgc
     1921 gtaaagacgg ccacaccgtc gtcgacgctc gccgcgccgt agaccgtgcg gccgtcgggg
     1981 ctgaccgcga cggaccggat gccgttgacg gcgcgagcgt ccgtgcagcc gtccgcgacc
     2041 gtcgaggcca cgcagccggc agcgccgtcg ggctgggtga gccggccggt gcccgggtca
     2101 cgcgcgaaca ccgcgatcgc gctgtcgctg aacgccgccg tgtagacgct gcgaccgtcc
     2161 gggctcacgg cgatgtcgaa gcccgcggtc aggccgcgca cggcgcggca gccctccgtg
     2221 ccggggtcgc tcacgcagcc gtccgcgccg ggcacccgcg tcagcgcgcc ggtcgcctcg
     2281 tcgcgcgtga acgtctggat ggttgcgctc gcgttcgaga agacgtacac gtgccgcccg
     2341 tcgggcgacg tcgccaccgc cttcgcggcc acgaggccgg cgccgtcgaa gcagctgccg
     2401 ccggtcccgc tctcgctgat gcagccgagc tgccccggga gctgcgcgag gttgcccgcg
     2461 gcagcggcgg tcgcggcgct gaccgcgaac gcggcgagca cgatcgcgag cggcaggagg
     2521 gtgcgccggg ccagggacac gcggccaccc taaccgggga gggggcgcgg cgccagtggg
     2581 tctactcgaa gcgcagctcg gacagcttcg cccagcgcgg gttcggctcg gcctcgtgcg
     2641 cgtggccggg atcggcgcga ttcaggttct cgccgcagac cgggcagagc ccgcggcagt
     2701 cggggccgca cacgatctga gtcggcagcg cgagcgccag cgcgtcacgc gcccatgcct
     2761 ggagatcgag ctcgtcgccg gtgacgtagg gcgagctcag ctcctcgtcg tcctcgccgg
     2821 gctggtcgat ctcgcgcgcg tcgacgtcca ccagcggtgc cgcggcgtcg aggcagcgca
     2881 tgcacgggcc gtcgagctcg gcctcgaagc gcagccgcag ggagtagccc gagacggtcc
     2941 gcgcagcgtc caggcgcacg tccaccaggt catcggccac ctggtactgc tgggcgccga
     3001 actcgaacgt gtcgagcttc acctggaggt cgatcgagcg ggccgagccc gagctgagct
     3061 ggagcctgcc gagctcgaag acgtcggtct gcgcggacac tcaggcgagg gtagccccgg
     3121 cggctcctcc tgtaggttgc gcgcggatga ggctctaccg cgcgccgttc tccaccaact
     3181 ccgagcgggt ggcgctcgcg ctcgcgcaca agggcatcga ggtcgagtcg gtctggatcg
     3241 actacggcga ccgcggggcg gtcgaggccg tgagcggcca ggggctcgtg ccggtggtcg
     3301 aggacggcgg cgaggtggtc ttcgactcgc cgcggatcct cgcgtggctc gaggagcgcc
     3361 ggcccgaccc gccgctgttc ccggcgagcg acccgcgccg cgccgagctc gaggtgttcg
     3421 tggactggtt caacgccgtc tggaagccgt ggccgaacgg gatcgaggcc gagctgggca
     3481 aggtcctgcc cgatcaggag ctgatcgacg cgcatgtcgc gtcgatggcc gacgcgctcg
     3541 accagttcga gcggctgctc gccggacgcg accacctgat gggcgacgag ttctccgtgg
     3601 ccgactgcgt ggtcttcccg ttcgtcaagt tcgcgctgcg gcgcgacccc gaggacgacg
     3661 agctcttcca tcgcgtcctc gacgactacc agtcggtcga cggccggccc cggctggagg
     3721 cgtggatcga gcgcgtcgac gcgaggccgc gcgcgtgagg cgcgcgctcg cgtgccttgt
     3781 ggccgtggcg gcgctcgcgg ggtgcggcgg cggcggggac ggcggcggct ccacgaagag
     3841 cgagcagccg gccgagcgga ccggcgcgat caccaaggac cccgcggccg agcgcgtcct
     3901 gcgcgatgcc tccgacgcgt tcaccaaggg ccgcgccgac atgctcgcct cgctccagac
     3961 cgacctgcgc cagaacgaca cgcagacgct tgtggacgac ctctgggacc tccgcaacgt
     4021 gatctacaag ttcgaccagg cgctcaggcg gatcgagttc gcgccggggc gggcggagcg
     4081 ggtctcggtt gagatcctgg agatagaccg cggggcgatc gcgcggatcg acccgatcct
     4141 cgacgccaaa gagcccccgc cgggcctcgc ggatgcggtc cagcaggctg cgacggacgc
     4201 cgccgcgatc gagcgcaaga cccgcgacct gatcgcggga accggctagc cgacttcggc
     4261 ttcttcgcgc ccggcgagcc ggtcgcggcc gcgctgcact gccgcggtga acttctggag
     4321 gttgacctcg agcgtgttga ggatgtcgtc ggcgtagtcc tcggcgccga gccggatctc
     4381 gcgctcgcgc gtgcgcgcct cctcgattat ctcctcggcc tggcgctcgg cctgacgcac
     4441 gacctcctcg gaggcgatca ggcgggcctg ctcctcgcgc gactccttga ggatccgctc
     4501 ggcctcgcgc ttggcctcgg ccagcatctc ctgacgctcc ttcacgatcc agcgcgcctg
     4561 cttgatctcc tcggggatcg tcgcgcgcat ctggtcgagg atgtcgtaga tctcctcctt
     4621 gtcgacacgc acctgatccg tcagcgggac gggcttggcg ttgtgcaccg tgtcgtcgag
     4681 cttgtctatg aggactagga catccatgag agcctcagtt cgtcagcgcg ggcggtgttc
     4741 cttgcgaaaa acccgcgcgc atctgttggt agatctccga gagcgtggcg cgttcgacgc
     4801 gtacctcgtt cgtaaggggc accggatgag cattgtggag gaggtcgtcg agggcgtcga
     4861 tcgccgggag gagggccacc gggccccacc cgcgcagctc ctcgacgacc ttggctacgg
     4921 cgcccttgtc gagccggacc tgcgtggtca gcgggaccgg gcgggccttg cggaccagcg
     4981 tgtcgaggcg gtcgaacgct gtgcgggccc ggcgggggtc gacggcggtc cgctcgaact
     5041 cctcgcgcgt gacgtagcgg tcctgcgcgg ctccctgccg gacccggccg acgatcgcga
     5101 gcagccggtc gcggtccacg cggaccgagc ccgtcagcgg gacggacttc gcgtcataca
     5161 tgaaggtgtc gagctcgtcg agggggacga gcgcgcccgc tccccggtac gtcccatggt
     5221 cctcgtccag gagaacgaac tcgatgctcg cgcgcatctg gtcgacgagg tcgtacacgc
     5281 tctcgcggtc gatgcgcgcc ccgccgagga tcggcgtctc cttcgcgtcg cggatcatgg
     5341 cctcgagctg gtcgacgagc tggtgcagcg acatgcgctc aacgcctcag cgcctcttcc
     5401 aggcgcggca gcacgctgtc cgggaccagc ccggacaggt cgccgccgaa catcgcgagc
     5461 tccttgaccc cgctcgagct caggaacgtg aactgcggcg aggagaacat gtagatgctc
     5521 tcgatgtcag gcgccatctt gcggttgagc tggttcatct cgaactcgta ct
//
LOCUS       DASVPL010000276         9273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_386930, whole genome shotgun sequence.
ACCESSION   DASVPL010000276 DASVPL010000000
VERSION     DASVPL010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386930"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..766)
                     /gene="cpaB"
                     /locus_tag="VF517_15405"
     CDS             complement(50..766)
                     /gene="cpaB"
                     /locus_tag="VF517_15405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX8054374.1"
                     /translation="MTRSARRRRGALLLALALASGGLASSRVERRERGIEARVGEPVP
                     VVVAAADLRQGARIGERQAAEVLAVRQVPAQFAPPDALGDPQQAVGATLAAPVAAGGY
                     LTAGAIAAPSSEGDESQAALAPGQRAVQITAAGGEELANAGPGALVDVVVTTERAEGR
                     GRTYLALERVELLDARPAPEGAAREDGAPATLATLRVTLRQAVFLAAAQTFAREIRLL
                     LRAPAERRRSGPLVVDSGAL"
     gene            complement(763..1536)
                     /locus_tag="VF517_15410"
     CDS             complement(763..1536)
                     /locus_tag="VF517_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8054375.1"
                     /translation="MIAAAGWGVAAAAAARVAVLRRQLRDAERRLDAGADAEHELRGA
                     VTAFGLALDRLARDPRGRRLAMSLGSELERARTALGDLGAARQGEPAAPRSERFALDR
                     LARSAAAAWQPAALAAGRRVELDWRAGPASVRGDRGRLAQALGNLLANAVEHGTGPIR
                     LEARRTGGRVRLEVVNGLREARDEAAAGGPAAGRRLGEAALDVSACAEPSGRRLGEAA
                     PRGRGLRIASRAVAASGGSLSIARGGGRASAAIELPLGG"
     gene            complement(1533..2246)
                     /locus_tag="VF517_15415"
     CDS             complement(1533..2246)
                     /locus_tag="VF517_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8054376.1"
                     /translation="MDTTLQRSVLVVEDDERTQAFLCDQLAADGFRVAAAAGVGEGLR
                     AMEVRRPDVVLLDLMLSDGHGLELLDRVRAADGVASRVDPDTPVVVLSGRAGEADRIR
                     GLTRGADDYVCKPFSYAELLARLRGVLRRASGRHLRGLLRVGDLTVDPATRAVRLGGR
                     PVVLSAKEFALLHALAADPARVHPKAELLRDVWGYRTAGTTRTVDTHACRLRRKLADG
                     EGNFVVNVRGVGYRLTEAA"
     gene            complement(2702..3508)
                     /locus_tag="VF517_15420"
     CDS             complement(2702..3508)
                     /locus_tag="VF517_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054377.1"
                     /translation="MLLDAIRAEPGITRAGMLAVTGLPSGAIDPARLRLWKAGLIEPK
                     DDKGWDDALNHRVKEVGWRFVDDPDRQALVRERAGARTERNAEPSAEEHAIVIVEALK
                     DPVVNRLVLEMTKEGTGSRRAQKRAEQALRAQAAARKRQADEAKREGAANSDFKRNLL
                     RLWEARGAVGAIDQHLIEERARVARGAPRRIADLDWVLALNDVRSIITSFGSMWQNLR
                     DIGGRNEPCPACGTIPAEPNRALGTFVVEGTATELTDVDEDVIDAGEAET"
     gene            complement(3602..4315)
                     /locus_tag="VF517_15425"
     CDS             complement(3602..4315)
                     /locus_tag="VF517_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054378.1"
                     /translation="MNTVASAHTADEIAARYTAADAEFKQAVEDRVYKYAADSDLLDE
                     NGDIDPERIVERAYEELVTKEVVNIDPGNDDRYNPATSSTKEELAASVFTGGPTAADA
                     ERNPVERVAYDKCLSLVWNHTVPTQRGRIQKRLEADKLLLVRGKVYRNGNTIGTGVFV
                     TKNPELVVREYLGPRLEKLRKLTEALEADFDLALERDKSLEGAMSAAIESAVAQAAGR
                     LPVTALGAGGDNGQKALGK"
     gene            5000..5638
                     /locus_tag="VF517_15430"
     CDS             5000..5638
                     /locus_tag="VF517_15430"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8054379.1"
                     /translation="MTVPADITDPRIAKAYAHPLRIHILGLLDERTASPSEIANELGA
                     PLTHVSYHVRQLAGLGLIRLVRTTPRRGAVEHHYTANIRPKVNDDAWGQIPDVVKKNL
                     VAGWVQQTGAHVSDAVKEGGFDRENAHMTRSPFDLDEKAWNAIAKELGKTQERIERIG
                     AEAKKRLEKDPSTETFRATTTMMLFEGPSQAAGLPEDAGIRRHKRRRAKSSA"
     gene            5748..6230
                     /locus_tag="VF517_15435"
     CDS             5748..6230
                     /locus_tag="VF517_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054380.1"
                     /translation="MSDAALGMDTTRRGNYESGSDYVLEYGELRFTFNERDFTERVEQ
                     AAIKLGFVDNRLDGAEREDLVNLAVNGEVSEPASALGEHIVECWGQLAGPSERSLVHW
                     LRRLVFRGAWLDQRVKEGELEVVFDERTHTFGYAQPDRDFEVIELSPEPSWSGVAYRR
                     "
     gene            complement(6250..7449)
                     /locus_tag="VF517_15440"
     CDS             complement(6250..7449)
                     /locus_tag="VF517_15440"
                     /inference="COORDINATES: protein motif:HMM:NF019138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEX8054381.1"
                     /translation="MAILDRAELERSPLADLHAIASELSIEGYRLLRRDELIDALLKQ
                     TGGDTSKPSRSRASSENGDADERPRSGRGRGGRPRTRKPRSDEDDATASDRADEAEAE
                     ADARGETQPLGEDARADDIGDDGPEESRTGVLDVLPNGSGFMRPDPFTHSREDVYVSP
                     AQIRRCELRAGDEIAGPVRAPRRSERYPSLVRVETINGAEADPPPARPRFDELTPVFA
                     TERLPAPDELSSAPFGRGSRVAVGGPPGSGITTLLRRIALALAEVDDLELTVVLAGVR
                     PEEVTEWRREEKLTVVGGAFDRPVEEQAQAAEIAVERGKRAVESGRHAAIVLDSLDAL
                     PPAAARRVFGAARCLEEGGSLTVIASTGLAGEPQRLATTQVILEAGADGPKLATGSGT
                     LHADRLS"
     gene            complement(7486..8313)
                     /gene="folP"
                     /locus_tag="VF517_15445"
     CDS             complement(7486..8313)
                     /gene="folP"
                     /locus_tag="VF517_15445"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX8054382.1"
                     /translation="MTTLMGIVNASPESFSDGARVGDLEGQVRRALELRDDGAGIIDV
                     GGESGVTDRAPVSVEEEIARVVPLVERLVGEGVTVSVDTWKPAVARAALGAGATMVND
                     VSGLRDPELADACAEAGAALVITHTGAPPKQKLFPVYEDVAATVVEFLRERIALARSR
                     GVERLVLDPGPDLGKTPADTVAALRALPRVAELGHPVLLAVSRKDFVGAITGRPPAER
                     AAGTLAAIGAGLDAGASILRVHDVRATADYVAVRAALRGEREVSPELRLDPSLRRAI"
     gene            complement(8310..>9273)
                     /locus_tag="VF517_15450"
     CDS             complement(8310..>9273)
                     /locus_tag="VF517_15450"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8054383.1"
                     /translation="NASGVWADTIRSEEVRDAAEVPRIRPSRGTHVTLAHETLPLNAG
                     AIVPAGGGRSIFALPWLGRTLIGTTDNDYEGGLEHIPADDDDVAYLLNACNEFFGLSL
                     DRGDLTGAYAGVRPLIATGDSKKSVDISRRAELFETRSGLVTITGGKLTTWRRMAKLV
                     VDRVVEREGREAPCRTHEIPLGEAADELEGPEGVDEGSLALLAGRYGYAAHDVLALVA
                     ERPELGRRVVPDLPDLLAEAPFAARYEQAASVADVLLRRTRLGILAAPDLTAEDSPAA
                     RAVAEALGEELGWDAGRVDHELDEWRAVARAERVNVNAEAAPVA"
BASE COUNT         1263 a         3481 c         3256 g         1273 t
ORIGIN      
        1 ggtgacggtc cggtggcggg gtttcgcgcg cgcacgcgcg agcgcgggat cagagggcgc
       61 cgctgtccac gacgagtgga ccgctgcggc ggcgctcggc gggtgcgcgc agcagaaggc
      121 ggatctcgcg ggcgaaggtc tgggcggcgg cgaggaagac ggcctggcgg agcgtgacgc
      181 ggagggtggc cagggtggcg ggcgcgccat cctcgcgggc ggcgccctcg ggggcggggc
      241 gggcgtcgag gagctcgacg cgttcgaggg ccaggtaggt gcgcccgcgg ccctccgcgc
      301 gctcggtggt gacgaccacg tccacgagcg cgccggggcc cgcgttcgcc agctcctcgc
      361 ctccggcggc cgtgatctga accgcgcgct ggcccggcgc gagggctgct tggctctcgt
      421 cgccctcgct tgagggcgcg gcaatggcgc ccgcggtgag gtagcccccg gccgccaccg
      481 gggccgcgag cgtggccccg accgcctgct gagggtcgcc gagcgcgtcg ggcggggcga
      541 actgggcggg cacctggcgc accgcgagca cctccgcggc ctggcgctcg ccgatcctcg
      601 cgccctgccg caggtcggcc gcggcgacca ccaccggaac cggctcgccc acccgcgcct
      661 cgatgccccg ctcgcgccgc tccacgcgcg acgaggcaag cccgcccgag gccagcgcga
      721 gtgcgagcag cagagccccg cgccttcggc gagcggaccg cgtcatccgc ccagcggaag
      781 ctcgatggcg gccgaagcgc gtccgccgcc gcgcgcgatc gagagcgatc cgccggaggc
      841 ggccacggcg cgggaggcga tgcggagccc gcggccgcgg ggggcggctt cgccgagccg
      901 gcgcccggac ggctcggcgc acgcggatac gtcgagggca gcttcgccga gccggcgccc
      961 cgcggcggga ccgcccgcgg cggcttcgtc gcgcgcctcg cggagcccgt tcacgacctc
     1021 cagccgcacc cggcctccgg tgcggcgggc ctcgaggcga atcggcccgg tcccgtgctc
     1081 cacggcgttc gccagcaggt tgccgagtgc ctgcgcgagc cggccgcggt cgccgcgcac
     1141 cgacgccggg cctgcccgcc agtcgagctc cacccgccgc ccggcggcca gcgccgcggg
     1201 ctgccaagcc gccgccgcgg aacgcgcgag gcggtcgagc gcgaaccgct cgctccgcgg
     1261 cgccgctggc tcgccctgcc gcgcggcgcc gaggtcgccg agggccgtcc gagcgcgctc
     1321 gagctcgctg ccgagcgaca tcgccagcct gcgaccgcgc ggatcgcgcg ccagccggtc
     1381 gagcgcaagc ccgaaggcgg tcaccgcacc gcgcagctcg tgctcggcgt cggcaccggc
     1441 gtcgagccgc cgctcggcgt cgcgaagctg gcggcggaga accgcgaccc gcgcggcggc
     1501 ggccgcggcg acaccccacc cggccgccgc gatcacgccg cctccgtgag ccggtagccg
     1561 acgccccgca cgttcaccac gaagttcccc tcgccgtccg cgagcttgcg ccgcagccgg
     1621 caggcgtgcg tgtcgacagt tcgcgtggtc cctgcggtcc tgtagcccca cacgtcgcgc
     1681 agcagctccg ccttcgggtg cacgcgcgcc ggatcggcgg caagtgcatg cagcagcgcg
     1741 aactccttgg ccgacagcac gaccggccgc ccgccgagac gcacggcgcg cgtggctggg
     1801 tcgacggtga ggtcgcccac ccgcagcaag ccgcgcaggt gccgccccga cgcgcgccgg
     1861 aggacgccgc gcaggcgcgc cagcagctcg gcgtaggaga acggcttgca gacgtagtcg
     1921 tcggcgccgc gggtgaggcc gcggatgcga tcggcctccc ccgcccggcc gctcagcacg
     1981 accacggggg tgtccgggtc gacgcgcgag gccaccccgt cggcggcccg tacgcgatcg
     2041 agcagctcga gcccgtgacc gtcggagagc atcaggtcga gcagcaccac gtccggccgg
     2101 cgcacctcca tcgcgcgcag cccctccccc acgccagccg cagccgcgac gcggaacccg
     2161 tcggcggcga gctggtcgca taggaatgcc tgggtgcgct cgtcgtcctc gacgaccaga
     2221 accgacctct gaagggtggt gtccacgcat ccctcagtgg cgccggctcc gttctcgccc
     2281 cccgcgcccg gccgacagag aaggccgaac gtggagagct gcgggacacg accgtccccg
     2341 ttgcggccct gcggagcaag cggctccccc aactaggacg cacgcgcgat gcggcggggg
     2401 tcgacctcta cccacgcgat ctcggcacac cactcgacag ccgcacgccg atgcgctctt
     2461 acgcgcacaa gatcccggtc ggacgaagac cgcgtgttcg acgcctacat acggcaccac
     2521 ttctcggaac gctgttgtgc atgagcgccc gactggcagg tgaggcgccc gccggcgaag
     2581 tcttgacctc gccggcgggt cccatcggcg gcggaggggc caggtctctc ggagacgcgg
     2641 ccgcagcgca tagccggttg cgggaactca ccccacgcgg cgcccttcgg cgccgccagg
     2701 cctaggtctc agcctctccg gcgtcgatca cgtcctcatc gacgtcggtc aactcggttg
     2761 ccgtgccctc gacaacaaaa gtccccaggg cgcggttcgg ctctgcgggg atggtgccgc
     2821 atgcggggca cggctcattc ctcccgccta tgtcacgtag gttttgccac atcgagccga
     2881 agctcgtgat gatcgaccgc acgtcgttga gcgccaggac ccagtcgaga tcggcaatcc
     2941 gccgcggcgc cccacgtgcg acccgtgcgc gctcttcgat taggtgctgg tcgatggcgc
     3001 cgacagcccc tcgcgcctcc cacaaacgaa ggaggtttcg cttgaagtcg gaattcgccg
     3061 ctccttctcg cttcgcctcg tccgcctggc gcttccgcgc tgccgcctgg gcccgcagcg
     3121 cttgctcggc acgcttttgc gcgcgccgtg agccggtccc ttccttagtc atctcaagga
     3181 cgagcctgtt gacaacaggg tccttaagtg cctcgacgat cacgatcgcg tgctcctccg
     3241 cggagggctc ggcgttccgt tcggttcgcg cgccagcccg ctcccggact agggcttgac
     3301 gatccgggtc gtcaacgaaa cgccacccga cctccttgac tcgatggttc agggcgtcgt
     3361 cccatccctt gtcgtccttc ggctcgatta gcccggcctt ccacagccta agacgagcgg
     3421 ggtcgattgc gcctgacgga aggccggtga ccgcgagcat ccctgctcgc gtgatccccg
     3481 gctctgcccg gatcgcatcg aggagcacgt cgcgagttgc tctattgctg gtggtagcca
     3541 tagctcatta cgagagcgaa gagctgacgc gcccgaacgc gcgccagcca gccgcgatct
     3601 cctacttgcc gagagccttc tggccgttgt ctccgcccgc gccgagcgcg gttacgggca
     3661 gcctccccgc agcctgcgcc acagccgact ctatggcggc gctcatggcc ccctcgagcg
     3721 acttgtcacg ctcgagtgcc aggtcgaaat cggcctccag cgcctcggtg agcttgcgca
     3781 gcttctcgag ccgcgggccg aggtactcgc gcacgacgag ctccgggttc ttcgtgacga
     3841 acacacccgt gccgatcgtg ttgccgttgc ggtagacctt cccgcgtacc agaaggagct
     3901 tgtccgcctc tagacgcttc tggatgcggc ccctctgcgt cggcaccgtg tggttccaga
     3961 cgagcgagag gcacttgtcg tacgccaccc tctcgaccgg gttgcgctcg gcgtccgcgg
     4021 cggtcggccc tccggtgaaa accgaggcgg cgagctcctc ctttgttgag ctcgttgccg
     4081 ggttgtaccg gtcgtcgttg ccggggtcga tgttcacgac ctccttcgta acgagttcct
     4141 cgtaggcacg ctctacgatc ctctcgggat cgatgtcccc gttctcatcg agcaggtccg
     4201 agtcggccgc gtacttgtac acgcggtctt cgaccgcctg cttgaactct gcgtccgctg
     4261 cggtgtatcg ggcggcgatc tcgtcggcgg tgtgcgccga ggcgacggta ttcacgtcgc
     4321 atcactctcc ttagccccga gtggctcagg gtcattgcgc gacgagagcc cagggagcgc
     4381 ttggttgagg ggcagcttat accacggccc gggccgagcg aaggacgacg tcgggaagat
     4441 cacgctcgtt gggtgaatgt ccgccctcag gtcggcgctt accccgcccc ggtcgagcgc
     4501 gtatacccct gtcgacgcct agcacgcgct tggcgccggg cgcaacgaac gaccaaggca
     4561 tcgcaggtaa aggctaggtg gagggaacgg gggaccatgg cgcgtcgact gcctagcgcc
     4621 gctagacgcg cccgaactac gcgagctcga agcgctcttt cgacgttgct ttcgcattcg
     4681 tgacacccga atcccttgca agggcacgga gccgaagaac cgcgtcctga cgaggtgatg
     4741 acctcgcggg cccagcgcgg gctagcggtc gtgcttccgc cgccggacca actccgagcc
     4801 cctccgcagc gcttctcacc agcccaccgc agccccgcag gcccgcaatc ccgcttccca
     4861 cctagcgtga acgcgccctt cgccacccca ttcgagcgct aagaagtccc ctcattccgt
     4921 agaacggtcg tgcattggcg cgttcggcga tgcgccacga ccccccccat tagtacgatc
     4981 cgctttgagg taacccacat tgaccgttcc cgcagacata actgacccac gtatcgccaa
     5041 ggcgtacgcg cacccgctcc ggatccacat cctcgggctg ctcgacgaaa ggaccgcgag
     5101 ccccagcgag atcgccaacg aactgggcgc gccgctgacg cacgtgagct accacgtccg
     5161 ccagctcgcc ggtctcggcc tgatcaggct cgtgcggacc accccgcgcc gcggcgccgt
     5221 tgagcaccac tacacggcga acatccgccc gaaggtcaac gacgacgcct ggggccagat
     5281 cccggacgtc gtgaagaaga acctcgtcgc gggctgggtc cagcagaccg gcgcgcacgt
     5341 atcggacgcc gtcaaggagg gcggcttcga tcgcgagaac gcccacatga cgcgcagccc
     5401 gttcgacctc gacgagaagg catggaacgc gatcgccaag gagctcggca agacgcaaga
     5461 gcgaatcgag cggatcggcg ccgaggcgaa gaagcggctg gagaaggatc cgtcgacgga
     5521 gacgttccgc gccaccacca cgatgatgct cttcgagggc ccgtcgcagg cggccggcct
     5581 ccccgaggac gccggcatcc gcaggcacaa gcgccgccgc gctaagtcct ccgcctagcc
     5641 cgtagcgccg acgcgccgtc cccgcggacg tcgtgcaatc tcggttgcac ggacccttcc
     5701 gcgggaggaa tcgaggttac gatgccgacg cgtcgcagcg ggggcccgtg agcgacgcgg
     5761 ccctcggcat ggataccacg cgacgcggaa attacgagtc cggctctgac tacgtactcg
     5821 agtacgggga gctgcgcttc acgttcaacg aacgtgactt caccgagcgc gtggagcagg
     5881 cggcgatcaa gctcggcttc gtggacaacc gcctcgacgg cgccgagcgc gaggatctcg
     5941 tcaacctggc cgtgaacggc gaggtgagcg agcccgcatc ggcgctcggc gagcacatcg
     6001 tggagtgctg gggccagctc gccggcccgt ccgagcggtc actggtgcac tggctgcggc
     6061 ggctcgtctt ccgcggggcc tggctcgacc agcgcgtgaa ggagggcgag ctcgaggtcg
     6121 tcttcgacga gcgcacccac accttcgggt acgcgcagcc ggaccgcgac ttcgaggtga
     6181 tcgagctctc gcccgagccc tcgtggtcgg gcgtcgcgta ccgccgctag tagcgcgagg
     6241 cgccgccggc tagctgagcc ggtccgcgtg cagcgtgccc gagccggtcg cgagcttggg
     6301 cccgtcggcg ccggcctcga ggatgacctg cgtggtggcg agccgctgcg gctcaccggc
     6361 caggccggtc gaggcgatca ccgtcagcga tccgccctcc tccaggcacc gcgccgcgcc
     6421 gaacacccgc cgcgcggccg cggggggcag cgcgtcgagc gagtcgagca cgatcgccgc
     6481 gtggcggccg gactccacag cccgcttgcc gcgctcgacc gcgatctcgg cggcctgcgc
     6541 ctgctcctcc acggggcggt cgaacgcgcc gccgacgacc gtcagcttct cctcgcgacg
     6601 ccactcggtg acctcctccg ggcggacgcc ggcgaggacc accgtcagct cgaggtcgtc
     6661 cacctccgcc agcgcgagcg cgatccggcg tagcagcgtc gtgatgcccg agccgggggg
     6721 tccgccgacg gcgacgcgcg agccgcggcc gaacggcgcc gaggacagct cgtcgggggc
     6781 gggcagccgc tccgtcgcga acaccggcgt cagctcgtcg aagcgcgggc gcgcgggcgg
     6841 cgggtcggcc tccgcgccgt tgatcgtctc gacgcgcacg agcgacgggt agcgctccga
     6901 gcgccggggc gcgcgcaccg ggccggcgat ctcgtcgccg gcgcgcagct cgcagcggcg
     6961 aatctgcgcc ggcgagacgt acacgtcctc gcgcgagtgg gtgaacgggt ccgggcgcat
     7021 gaagccgctg ccgttcggca gcacgtcgag cacgccggtg cgcgactcct cggggccgtc
     7081 gtcgccgatg tcgtcggcgc gagcgtcctc gccgagcggc tgggtctcgc cgcgggcgtc
     7141 ggcctcggcc tcggcctcgt cggcgcgatc ggaggcggtc gcgtcgtcct cgtcggagcg
     7201 cggcttgcgg gtgcgcgggc gcccaccgcg cccgcgccct gagcgcgggc gctcgtcggc
     7261 gtcgccgttt tcggacgagg cacgggagcg actcggcttg ctcgtgtcgc cgccggtctg
     7321 cttgagaagc gcgtcgatca gctcatcgcg tcgcaagagg cggtagccct cgatgctcag
     7381 ctctgaggcg atcgcgtgca ggtcggcgag cgggcttcgc tccagctcgg ctcggtcgag
     7441 gatcgccacg tttcagtctc cttgggttac ggggccggag agggctcaaa tcgcgcgtcg
     7501 aaggctcgga tcgagcctca gttccggcga aacctcccgc tcacccctga gggccgcgcg
     7561 caccgccacg tagtcggcgg tggcgcgaac gtcgtggacc cgcaggatag acgcccccgc
     7621 gtcgagtccc gctccgatcg cggccagcgt cccggcggcg cgctccgcgg gagggcggcc
     7681 ggtgatcgcg ccgacgaagt ccttgcgcga taccgcgagc agcaccgggt gcccgagctc
     7741 ggccacgcgg gggagcgccc gcagcgccgc gacagtgtcc gccggcgtct tcccgaggtc
     7801 cggaccgggg tcgagtacga gccgctcgac cccgcgcgag cgcgcgagcg cgatccgctc
     7861 gcgcaggaac tcgaccaccg tggcggccac gtcctcgtag accgggaaca gcttctgctt
     7921 cggcggcgcg ccggtgtggg tgatcacgag cgccgcgccg gcctcggcgc acgcgtccgc
     7981 cagctcgggg tcgcggagac cgctcacgtc gttgaccatc gtggccccgg cgccgagcgc
     8041 cgcgcgcgcg accgcgggct tccacgtgtc caccgagacc gtgaccccct cccccacgag
     8101 gcgctccacc agcggcacga cgcgcgcgat ctcctcctcg acgctcaccg gcgcgcgatc
     8161 ggtcacgccg gactcgccgc cgacgtcgat gatcccagcg ccgtcgtcgc gcagctcgag
     8221 cgcgcgccgc acctgcccct ccagatcgcc cacgcgcgcg ccgtccgaga acgactcggg
     8281 gctcgcgttc acgatcccca tgagggtcgt catgccaccg gcgcggcctc ggcgttcacg
     8341 ttcactcgtt cggcgcgcgc caccgcgcgc cactcgtcca gctcgtggtc gacccggccg
     8401 gcgtcccagc ccagctcctc gccgagcgcc tcggccaccg cgcgcgccgc cgggctgtcc
     8461 tcggcggtca ggtcgggcgc ggcgaggatc ccgaggcgcg tgcggcgcag cagcacgtcg
     8521 gccacgctcg cggcctgctc gtagcgggcg gcgaacggcg cctcggccag caggtccggc
     8581 aggtccggca cgacccggcg gccgagctcc ggccgctcgg ccacgagcgc gagcacgtcg
     8641 tgggcggcgt acccgtagcg gccggccagc agcgcgagcg atccctcgtc gaccccctcg
     8701 ggcccctcga gctcgtcggc cgcctcgccc agcgggatct cgtgcgtgcg gcacggcgcc
     8761 tcgcgaccct cgcgctcgac cacgcggtcc accacgagct tcgccatccg ccgccaggtg
     8821 gtgagcttgc cgccggtgat cgtcacgagg cccgagcgcg tctcgaacaa ctcggcgcgc
     8881 cgcgagatgt ccaccgactt cttggagtcg ccggtcgcga tcagcgggcg gacgccggca
     8941 taggcgccgg tcaggtcgcc gcgatcgagc gagagcccga agaactcgtt gcaggcgttc
     9001 aggaggtagg ccacgtcgtc gtcgtcggcc gggatgtgct cgagcccgcc ctcgtagtcg
     9061 ttgtcggtcg tgccgatgag cgtgcggccg agccacggca gcgcgaagat cgagcggccg
     9121 ccgccggccg ggacgatcgc gcccgcgttc agcggcagcg tctcgtgcgc gagcgtgacg
     9181 tgggtgccgc ggctcgggcg gatgcgcggg acctccgccg cgtcgcgcac ctcctcggag
     9241 cggatcgtgt cggcccacac gccggacgcg ttc
//
LOCUS       DASVPL010000277         7647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_111931, whole genome shotgun sequence.
ACCESSION   DASVPL010000277 DASVPL010000000
VERSION     DASVPL010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111931"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VF517_15455"
     CDS             complement(<1..164)
                     /locus_tag="VF517_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX8054384.1"
                     /translation="MASLTQNKIRIRLKAYDHSAIETAAKEIVETATRTGATVSGPVP
                     LPTEKNVYCV"
     gene            complement(248..1435)
                     /gene="tuf"
                     /locus_tag="VF517_15460"
     CDS             complement(248..1435)
                     /gene="tuf"
                     /locus_tag="VF517_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX8054385.1"
                     /translation="MAKEKFERDKPHVNVGTIGHIDHGKTTLTAAITKVLSDTYGGQA
                     RSFEEIDNAPEEKERGITIATSHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDDELLELVEVEVRDLLSE
                     YDFPGEDVPFVTGSALKALEGDEAYTAKIIELAGHLDTYIPEPERPLDLPFLMPVEDV
                     FSITGRGTVATGRIEQGIVKTGEEVEIVGITEQVDKTIVTGVEMFRKILDEGQAGDNV
                     GCLLRGTKREEIQRGQVLCKPGSITPHTKFKAEVYCLKKEEGGRHTPFFNGYRPQFYF
                     RTTDVTGVANLPEGTEMVMPGDNVQMQIELIQPIAMDQGLRFAIREGGRTVGSGVVTE
                     IVE"
     gene            complement(1505..3613)
                     /gene="fusA"
                     /locus_tag="VF517_15465"
     CDS             complement(1505..3613)
                     /gene="fusA"
                     /locus_tag="VF517_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX8054386.1"
                     /translation="MPREFPLEKTRNIGIMAHIDAGKTTTTERILYYTGRTHKMGEVH
                     EGAAVMDWMEQEQERGITITSAATTAEWRDYRVNIIDTPGHVDFTVEVERSLRVLDGA
                     IALFDSVAGVEPQSETVWRQADKYRVPRIAYINKMDRIGADFEMGVKTMVDRLGAHPV
                     PVQLPIGAEGDFMGVIDLVAEQAIFYDDDLGKDVRVEAIPDEYKERAHDARTALIEAI
                     AEYDDDLMEAYLAEEEIEVDRLKADIRKATLDISMTPVLCGSSFKNKGVQPLLDAVID
                     YLPSPLDVPPVMGVEPVKGEEEGAPAARPPEEDAPFAALAFKVMSDPFVGKLTYFRVY
                     SGKLSAGGRVLNTKTGRTERAGRILMMHANDREEQDEIYAGDIAAAVGLKQTSTGDTL
                     CAPDAPIVLETINFPDPVVHVSIEPKTKVDQEKMGVALARLAEEDPTFQVRTDEETGQ
                     TVISGMGELHLEVIVDRMRREFNVEAGVGRPQVAYRETVRGEATKVEGKFIKQTGGSG
                     QYGVVYINLEPAPGEGFDFVNKIKGGSVPSEFIPAVEKGIEEALDTGVKAGYPMVDVR
                     ATLTDGKYHDTDSSEMAFKIAGSMALKAAAQKAKPVLLEPVMAVEVVVPQDFLGDVIG
                     DLSKRRGRVEGQEPRGNALAVAAKVPLSEMFGYATDLRSNTQGRANYTMQFDRYEEVP
                     TNIAETIVGERSGEPVAAGA"
     gene            3894..4349
                     /locus_tag="VF517_15470"
     CDS             3894..4349
                     /locus_tag="VF517_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054387.1"
                     /translation="MLAIVSEFSTISYVTVVTATCEDLADPRLADRCQLDGAERHSWA
                     FALLGGFGMLMAWGATVGRSRPAAIALALVGAALLGLGFFRDLPEAGRTGLIGEAYEA
                     TAHKGAGLWLELAAGVLMVVVGLVALLKLLRGPPASAASTEADAEPAAA"
     gene            4419..6578
                     /locus_tag="VF517_15475"
     CDS             4419..6578
                     /locus_tag="VF517_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054388.1"
                     /translation="MASCAAMGLGDYLLGVLLLALIAGPLVLGAICVRARVLPDWDGA
                     PARVAEAVLALAALILVAEALGTAGLLARAPLAVAGIAAGAAAWKWGESPFRSAIGPR
                     NGLAPRPEQAGGRAAAAIALAACAAVAVAWFDRTDFAVHSGMTDPDTLWYHLPVAARF
                     AQEGSTTALHFTSYEPLTPFHPANGSLVYAVGMVAFGNDVLSPFVNLGWLALALVAGW
                     CVGRPFGAGPVTLLGTAIVVGSPILTTMDGGTAKDDVMGVALLLAAVALLVNGGGARA
                     PNAIAAIAGGLLLGVKLSMVVPALLLAVAAVVGAPRARRAATAGTAGTAGTWTIALFV
                     VGGYWYARNLARTGNPAPWVDFGPLGLNKPELAPYTERWVVGSISERAGEIDGFWGNW
                     FAPAAETALGPAWWAILILAVGGGLAALVAGRGALHRLLGAAAVLIAIAYVVTPGSAG
                     GSPDGVPHLVLWNMRHLTPALALGLVCLPLIGRLRGRAQTAVLLLYAAVFAATQFAGG
                     FFELWGARPTAVALLGGAGAVLLVAVAARIELRRLGVAAAIATVAVLGAGYKAQHIYF
                     DHRYAEAGSDLGPAYAWARDLHDARVAIVGFFLQYPLYGKDLSNHVQYVGIDGGDGEF
                     RSVTSCAEWRGRLATGDYDYVVTAPFNYPWGASRAYPREARWTETDPAAQRIGRSGPV
                     AIFRLTGKPDPSTCESDGFGAKTEVEEQQTGTRPPTD"
     gene            6675..>7647
                     /locus_tag="VF517_15480"
     CDS             6675..>7647
                     /locus_tag="VF517_15480"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX8054389.1"
                     /translation="MHTHARAKLGPAGRFALTEAIANGRSQKAAAAAFCVSPATAHRW
                     WHRRLAASEQELRSGSWLFDRPSRPRRSPRLLDGELQERICECRRHTGWGPRLVAGAT
                     GQAHSTVWKVLRRHGISRPQRAPKEPANRYEWPCPGDLLHMDVSRYARFERPGHAVTG
                     DRSQRSRRWMDPDTRVGYDFAHAIVDDHSRLAYVELHPDERAATVTAFVERALAWFER
                     QGIVTRRLMSDNAFSYVKNRSLSELLELRQIKHLRTQAYRPRTNGKVERFHQTMAREW
                     GYGLAYRSHHHRNRALPDWLAYYNERRPHSSIGGLAPISRVHNVRGQD"
BASE COUNT          996 a         2634 c         2740 g         1277 t
ORIGIN      
        1 atcacgcagt acacgttctt ctcggtcggc agcgggaccg gccccgacac cgtcgcgccg
       61 gtgcgcgtcg cggtctcgac gatctccttg gccgccgtct cgatggccga gtggtcgtac
      121 gccttgaggc ggatccggat cttgttttgg gttagggaag ccatggtgaa ttctcgacgg
      181 acgtgccgag ggcggactcc gccggagccc gccccctcgg tcctgcagcg ctattggctg
      241 ctgaggacta ctcgacgatc tcggtgacca cccccgagcc caccgtgcgg ccgccctcgc
      301 ggatggcgaa gcgcaggccc tgatccatcg cgatcggctg gatcagctcg atctgcatct
      361 ggacgttgtc gcccggcatg accatctcgg tcccctcggg gaggttcgcg acgccggtta
      421 cgtcggtcgt acggaagtag aactgcggcc ggtagccgtt gaagaacggg gtgtgacggc
      481 caccctcctc cttcttgagg cagtagacct cggccttgaa cttcgtgtgc ggcgtgatcg
      541 agccgggctt gcagagcacc tggccgcgct ggatctcctc gcgcttggtg cctcgcagca
      601 ggcagccgac gttgtcgccc gcctggccct cgtcgaggat cttgcggaac atctcgacgc
      661 cggtgacgat cgtcttgtcg acctgctcgg tgatgccgac gatctcgacc tcctcgccgg
      721 tcttcacgat gccctgctcg atgcggccgg tggcgacggt gccgcggccg gtgatcgaga
      781 agacgtcctc gacgggcatc aggaacggca ggtcgagcgg gcgctccggc tccgggatgt
      841 aggtgtccag gtggccggcg agctcgatga tcttggccgt gtaggcctcg tcgccctcga
      901 gcgccttgag cgccgagccg gtgacgaacg ggacgtcctc gcccgggaag tcgtactcgc
      961 tcagcaggtc gcggacctcg acctcgacga gctcgaggag ctcgtcgtcg tcgaccatgt
     1021 cggccttgtt gaggaacacg acgatgtacg gcacgccgac ctggcgggcg agcaggatgt
     1081 gctcacgcgt ctgcggcatg gggccgtcgg ccgccgagac gaccaggatc gcgccgtcca
     1141 tctgcgcggc gccggtgatc atgttcttga cgtagtcggc gtggcccggg cagtcgacgt
     1201 gggcgtagtg ccggttctcg gtctcgtact cgacgtgcga ggtggcgatc gtgatcccgc
     1261 gctccttctc ctccggagcg ttgtcgatct cctcgaagga gcgagcctgg ccgccgtatg
     1321 tgtccgacag gacctttgta atggcggcag tcagcgtcgt cttgccatgg tcgatatggc
     1381 cgatggtgcc gacgttcacg tgcggcttgt cgcgctcgaa cttctccttc gccactgtgt
     1441 cgtgtctcct tggtcggaaa gcttcgttcg cccgggggac gggcgaaccg gtgaaatgta
     1501 gctactaggc gcccgctgcg accggttcgc cggatcgctc gccgacgatg gtctccgcaa
     1561 tgttcgtggg gacctcctcg tagcggtcga actgcatggt gtagttggcc cggccctgcg
     1621 tgttcgaacg caggtcggtg gcgtacccga acatctccga gagcgggacc ttcgcggcca
     1681 cggccagcgc gttgccgcgc ggctcctgac cctcgacgcg gccgcgccgc ttcgagaggt
     1741 cgccgatcac gtcgccgagg aagtcctgcg gcacgacgac ctcgacggcc atcacgggct
     1801 cgagcagaac cggcttcgcc ttctgcgcgg ccgccttcag cgccatcgag ccggcgatct
     1861 tgaacgccat ctccgaggag tcggtgtcgt ggtacttgcc gtcggtgagc gtcgcgcgga
     1921 cgtcgaccat cgggtacccg gccttgacgc ccgtgtcgag cgcctcctcg atgcccttct
     1981 cgaccgccgg gatgaactcc gacgggaccg agccgccctt gatcttgttg acgaagtcga
     2041 agccctcgcc gggcgcgggc tcgagattga tgtagacgac gccgtactgg ccggagccac
     2101 cggtctgctt gatgaacttg ccctcgacct tggtggcctc gccgcgcacg gtctcgcggt
     2161 aggcgacctg cgggcggccg acgccggcct cgacgttgaa ctcgcgacgc atgcggtcga
     2221 cgatcacctc gaggtggagc tcgcccatgc cgctgatgac ggtctggccg gtctcctcgt
     2281 cggtgcggac ctggaacgtc gggtcctcct cggcgagccg cgcgagcgcg acgcccatct
     2341 tctcctggtc gaccttggtc ttcggctcga tcgagacgtg gaccaccggg tcggggaagt
     2401 tgatcgtctc gaggacgatc ggagcgtcgg gcgcgcagag cgtgtcgccg gtcgaggtct
     2461 gctttaggcc gaccgcggcg gcgatgtcgc ccgcgtagat ctcgtcctgc tcctcgcggt
     2521 cgttcgcgtg catcatcagg atgcgcccgg cgcgctcggt gcggccggtc ttcgtgttga
     2581 gcacgcggcc gccggcgctg agcttgccgg aatagacgcg gaagtacgtg agcttgccga
     2641 cgaacgggtc ggacatgacc ttgaacgcca gggcggcgaa cggggcgtcc tcctccggcg
     2701 gccgggccgc gggcgcgccc tcctcctcgc ccttgacggg ctccacgccc atgacgggcg
     2761 ggacgtcgag cgggctcggg aggtagtcga tcaccgcgtc gagcaggggc tgcacgccct
     2821 tgttcttgaa gctcgacccg cagaggaccg gcgtcatcga gatgtcgagc gtggccttgc
     2881 ggatgtccgc cttgaggcgg tcgacctcga tctcctcctc ggcgaggtat gcctccatga
     2941 gatcgtcgtc gtactcggcg atggcctcga tcagcgcggt gcgggcgtcg tgcgcgcgct
     3001 ccttgtactc gtccgggatc gcctcgacgc ggacgtcctt gccgaggtcg tcgtcgtaga
     3061 agatggcctg ctcggcgacg aggtcgatga cgcccatgaa gtcgccctcc gcgccgatcg
     3121 gaagctgcac ggggaccggg tgcgcgccga ggcggtcgac catggtcttg acgcccatct
     3181 cgaagtcggc gccgatgcgg tccatcttgt tgatgtaggc gatgcgcggg acgcggtact
     3241 tgtcggcctg gcgccagacg gtctcggact gcggctcgac gccggccacc gagtcgaaca
     3301 gcgcgatggc gccgtcgagc acgcgcaggg agcgctctac ctcgacggtg aagtcgacgt
     3361 ggcccggcgt gtcgatgatg ttgacgcggt agtcgcgcca ctcagcggtg gtcgcggccg
     3421 aggtgatcgt gatgccgcgc tcctgctcct gctccatcca gtccatgacc gcggcgccct
     3481 cgtggacctc gcccatcttg tgggtccggc cggtgtaata gaggatgcgc tcggtggtcg
     3541 tcgtcttacc ggcatcgatg tgcgccatga tcccgatgtt gcgcgtcttc tctagtggga
     3601 attcccttgg catctaaatc tctcgcttcg ccggctattt cgagcagaaa aaaggcccgt
     3661 ccagggcccc tcgtacaacc ttggatgttc cccgagggga gtctggttat tcgagggctt
     3721 tcatgggtgt ggacatgaac aggggacggc gcgaaccgtc cccctcaaga cggggcaact
     3781 ctagcactgg gattcggcgc cgcccacgga gtttcctgcg ttccgatgcg gcctggctgg
     3841 gtcgatcggg cctgctggcg tgggtgcttc tggccgccgg agtgggtgcc ggagtgctgg
     3901 ccatcgtgtc cgagttctcg acgatctcgt acgtgaccgt cgtgaccgcc acctgcgagg
     3961 acctggccga cccgcggctc gccgaccgct gccagctcga cggggccgag cggcactcgt
     4021 gggcgttcgc gctcctgggc gggttcggga tgctgatggc gtggggagcg accgtcgggc
     4081 gctcgcggcc ggcggcgatc gccctcgcgc tggtcggagc ggcgcttctg gggctcgggt
     4141 tcttccgcga cctgcctgag gcggggcgca ccgggctgat cggggaggcg tacgaggcca
     4201 ccgcgcacaa gggggccggg ctgtggctcg agctcgccgc gggggtgttg atggtggtgg
     4261 tggggctcgt cgcgctgctg aagctgctgc gcggcccccc ggcgtccgcg gcctccaccg
     4321 aggccgacgc ggagcccgcg gcggcctaac ggcggcactt agggggctgg tcgcggcgct
     4381 tccgcgcgcc ggggccgcgg cgggccagtc gtcgcggctt ggcatcctgc gcggcgatgg
     4441 ggctcggcga ctacctgctc ggcgtgctgc tgctcgcgct gatcgccggg ccgctggtgc
     4501 tcggcgcgat ctgcgtgcgg gcgcgggtcc tgccggactg ggatggcgcg ccggcccggg
     4561 tggccgaggc cgtgctggct ctcgccgcgc tgatcctcgt ggccgaggcg ctggggaccg
     4621 ccggcctgct cgcccgcgcg ccgctcgccg tggccggcat cgccgccggc gccgccgcct
     4681 ggaaatgggg tgagagtcca tttcggagtg ctataggacc cagaaatgga ctcgcgcccc
     4741 ggccggagca ggccgggggg cgcgcggccg ccgccatcgc gctggcggcg tgtgccgccg
     4801 tcgccgtcgc ctggttcgac cgcaccgact tcgccgtgca ctcgggcatg accgacccgg
     4861 acacgctctg gtaccacctg cccgtcgcgg cgcggttcgc gcaggagggg tccaccaccg
     4921 cgctccactt cacgagctac gagccgctca cgccgttcca ccccgcgaac gggtcgctgg
     4981 tgtacgcggt cgggatggtc gcgttcggga acgacgtcct ctcgccgttc gtgaacctcg
     5041 gctggctcgc gctcgcgctg gtcgccgggt ggtgcgtcgg ccggccgttc ggcgccggcc
     5101 ccgtcacgct gctcggcacc gccatcgtcg tcggctcgcc gatcctcacc acgatggacg
     5161 gggggacggc caaggacgac gtcatggggg tcgcgctgct gctcgccgcg gtggcgctgc
     5221 tcgtgaacgg aggcggcgcg cgggcgccga acgcgatagc cgcgatcgcg ggcgggctgc
     5281 tgctgggagt gaagctctcg atggtcgtgc ccgccttact gctcgccgtt gccgcagtcg
     5341 tcggcgcccc ccgcgcacgc cgagcggcca ccgcgggcac cgcgggcacc gcgggcacct
     5401 ggacgatcgc gctcttcgtg gtgggcggat actggtacgc gcgcaacctg gcgcggacgg
     5461 ggaaccccgc gccatgggtc gacttcggcc cgctgggcct gaacaagccg gagctcgccc
     5521 cgtacaccga gcgctgggtg gtgggctcga tcagcgagcg cgccggcgag atcgacggct
     5581 tctggggcaa ctggttcgcg cccgcggccg agactgcgct cggaccggcg tggtgggcga
     5641 tcctgatcct cgcggtcggc ggagggctcg cagccctggt ggcaggacgc ggagctctgc
     5701 accgcctgct gggtgcggcc gccgtgttga tcgcgatcgc gtacgtggtc acgccgggca
     5761 gcgcgggcgg gtccccggac ggcgtccccc acctggtcct ctggaacatg cgccacctca
     5821 ccccggccct ggccctggga ctggtgtgcc tcccgctgat cgggcggctg cgcggcagag
     5881 cgcaaacggc ggtgctcctc ctatatgccg ccgtgttcgc cgccacccag ttcgcgggcg
     5941 gcttcttcga gctgtgggga gcgaggccga ctgccgtcgc gcttctgggg ggagcgggtg
     6001 cggtgctcct ggtcgcggtg gccgcgcgga tcgagctccg ccgcctgggc gtggcggccg
     6061 ccattgcgac ggtggcggtg ctgggcgccg gctacaaggc gcagcacatc tacttcgacc
     6121 accgctacgc cgaggccggc tccgatctcg gccccgcgta cgcgtgggcc cgcgacctgc
     6181 acgacgcgcg agtcgcgatc gtcggcttct tcctccagta cccgctctac gggaaggacc
     6241 tgtcgaacca cgtgcagtac gtgggcatcg acggaggcga cggcgagttc cgctcggtca
     6301 cgagctgtgc cgagtggcgc gggcggctcg cgaccgggga ctacgactac gtggtcaccg
     6361 cgccgttcaa ctacccgtgg ggagcctccc gcgcctaccc ccgcgaggcg cgctggaccg
     6421 agacggaccc ggccgcgcag cgcatcggcc gctccggccc ggtggcgatc ttccgcctca
     6481 ccggcaagcc cgacccgagc acgtgcgaga gcgacgggtt cggagcgaag acagaggtgg
     6541 aggaacagca gacgggcacg cgcccgccga cggactagct gagccgccca cacaccttgt
     6601 gaacggctga acgacgtccg ggcctctcca gaggctgtgt gtctgcgacg actcaccgcc
     6661 caaggagggc ccggatgcac actcacgcta gagccaagct cggaccggcc ggccgcttcg
     6721 cgctgaccga ggcgatcgcg aacgggagat cgcagaaggc ggccgcggcc gccttctgcg
     6781 tgtcgccagc caccgcccac cgctggtggc accggcggct cgccgcgagc gagcaggagc
     6841 tgcgctccgg tagttggctg ttcgaccgcc cgagccggcc gcggcgcagc ccgaggctgc
     6901 tcgacggcga gcttcaggag cgcatctgcg agtgccgccg ccacaccggc tggggaccgc
     6961 ggctcgtcgc cggcgccacc ggccaggccc actcgacggt ctggaaggtg ctccgccgcc
     7021 atggcatctc gcgaccgcag cgcgcaccga aggagcccgc caaccgctac gagtggccgt
     7081 gccccggcga tctgctgcac atggacgtct cgcgctatgc gcgcttcgag cgccccgggc
     7141 acgcggtcac cggcgatcgc tcgcagcgct cgcgcaggtg gatggacccc gatacgcgcg
     7201 tcggctacga cttcgcccac gcgatcgtcg acgaccactc gcggctcgcc tacgtcgagc
     7261 tgcacccaga cgagcgggcc gcgacggtga cggcgttcgt cgagcgtgcg ctggcgtggt
     7321 tcgagcgcca ggggatcgtg acgcggcggc tgatgagcga caacgcgttc tcctacgtca
     7381 agaaccgctc gttgagcgag ctgctcgagc tccgccagat caagcacctg cgcacgcagg
     7441 cctaccggcc gcgcaccaac ggcaaggtcg agcgcttcca ccagacgatg gcgcgcgagt
     7501 ggggctacgg gctcgcctac cgctcccacc accaccgcaa ccgcgccctg ccagactggc
     7561 tcgcctacta caacgagcgc aggccgcaca gctcgatcgg agggctagcg ccgatcagcc
     7621 gcgttcacaa cgtccgtggg caggaca
//
LOCUS       DASVPL010000278         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_149462, whole genome shotgun sequence.
ACCESSION   DASVPL010000278 DASVPL010000000
VERSION     DASVPL010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149462"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VF517_15485"
     CDS             <1..587
                     /locus_tag="VF517_15485"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OpcA/G6PD domain-containing protein"
                     /protein_id="HEX8054390.1"
                     /translation="QEPDPQGAVERAREHLGNGYVVDLAWLRSAPWRERIASTFDPDV
                     WRPALFDIESVTIRHHPDSAISGLLLIGWLASRLGWETGSVIRRNGDLMARARAKRHE
                     VKLALESEPKLNVPGLDGLSIQCSRGMRIALSRGPGGLSAKRTLRDGSESSWVVMGAS
                     RGEQGILGEGIRQALLRDPTYGPALGAAASMLGT"
     gene            584..1243
                     /gene="pgl"
                     /locus_tag="VF517_15490"
     CDS             584..1243
                     /gene="pgl"
                     /locus_tag="VF517_15490"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX8054391.1"
                     /translation="MIRVVDDPAAEAAELIAAAVRAGGHIALAGGSTPKAAYGIVAGM
                     ELDWSGATVWFGDERCVPADDELSNYGMAREALLDRVEGATVKRMAGEKGPHAGAEDY
                     ERELRETLGEALPRLDLILLGLGPDRHTASLVPGKPAIEVKDRAVVGVEEAGMPPEVP
                     RITLTLPAINAARQVVFLVAGEDKSDAVQAAFGNGEGPAGLVDADVVLLDEGAASRLT
                     R"
     gene            1240..3168
                     /locus_tag="VF517_15495"
     CDS             1240..3168
                     /locus_tag="VF517_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8054392.1"
                     /translation="MTTDAGAPLTRPEVEAYDGQSAFPPIADYAFLSDCETVALVAPS
                     GNIEWFCLPRPDGPSVFGALLDRDAGGFRLGPADMTVPAGRRYIPGTNVLETTWMTRM
                     GWLVVRDALSVGPWHDVDERSHTHRRTPTDHDAEHVLIRTVHCVQGTVELHLDCEPAF
                     DYGRAGAEWRYAGDNYNEAVASADGVDVELRLTTDLRVGFEGRRARARHTLREGESAY
                     AALSWSKHAPPKDFQEAVDRMMRTEDFWRQWLNRGLFPDHPWRLYLQRSALTLKGLTY
                     APTGAMLAAATTSLPETPGGERNWDYRYSWIRDSTFMLWGLYTLGFDWEANDFFYFVA
                     DVAGDEGKDLQIMYGIGGEAELEEEELDHLTGYDGAKPVRVGNGAYTQAQHDVWGAVL
                     DSVYLHTRSRDLLPERIWPMLVHQVEAALENWRKPDRGIWEVRGDPKHFVSSKLMCWV
                     AADRGARLAAIREDDEAAERWAAAAEEIKDDICENGVDKRGVFTQHYETDALDASVLL
                     MPLVRFLPPDDERIVDTVRAVAEELTENGLVLRYRVDETDDGLAGEEGTFAICSFWLV
                     SALAEIGDHKSARQLCERMLSYASSLQLYAEEIDAVTGRHLGNFPQAFTHLALINAVM
                     HVIRADQDESTRRFSRAR"
     gene            complement(3165..3683)
                     /locus_tag="VF517_15500"
     CDS             complement(3165..3683)
                     /locus_tag="VF517_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054393.1"
                     /translation="MEPDPEPGPSPPPPPSADPDPAAEPEPAPEAEPEPAPEPEPEPE
                     PEPDPEPLPEPAPGTVTPGTEGALGAEGALGTEGADGTLGALGTLGAGTLGTLGAEGN
                     EAGAWSSGTALLTTSTLPARATPNASSATAAITNTTDRVTLPGIPAISSPFPVRPGAE
                     RAAAGASRDPAP"
     gene            3751..>4377
                     /locus_tag="VF517_15505"
     CDS             3751..>4377
                     /locus_tag="VF517_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054394.1"
                     /translation="MRAARARAREAAARERRVRRERRLRALVTRLTGCLDFLPESEGR
                     YLALRAGVGSERPQSRRAAARQAGIPAGRAATTERRGLRRLRRAARAGGCGAGSAGAS
                     VALTGATGPQLQPAVLMAPAPALRSAVEMAGGGEERDRQGVKAATASSESAEGAGGAG
                     DGDEQRSVLAPLADSVDEPSATGWYVLLAAVAALSVALLILQRRDRAAH"
BASE COUNT          552 a         1538 c         1714 g          573 t
ORIGIN      
        1 tgcaggagcc ggacccgcag ggggccgtcg agcgcgcgcg cgagcacctc ggcaacggct
       61 acgtcgtgga cctcgcctgg ctgcgcagcg ctccgtggcg cgagcggatc gcctccacgt
      121 tcgacccgga cgtgtggcgc cccgcgctct tcgacatcga gtcggtgacg atccgccacc
      181 atccggactc ggcgatcagc gggctgctgc tgatcggctg gctggcctcg cggctcggct
      241 gggagaccgg ctcggtgatc cgccgcaacg gcgacctgat ggcgcgggcg cgggcgaagc
      301 gccacgaggt gaagctggcg ctcgagtccg agccgaagct gaacgtgccc ggcctcgacg
      361 gcctgagcat ccagtgctcg cgcgggatga ggatcgcgct cagccgcggg cccggcgggc
      421 tgtcggcgaa gcgcacgctg cgcgacggca gcgagtcgag ctgggtggtg atgggcgcct
      481 cgcgcggcga gcagggcatc ctcggcgagg ggatccgcca ggcgctgctg cgcgatccca
      541 cgtacggtcc ggcgctgggc gccgcggcct cgatgctcgg cacgtgatcc gggtggtcga
      601 cgaccccgcc gcggaagcgg cggagctgat cgccgcggcc gtgcgggccg ggggccacat
      661 cgcgctcgcg ggcgggtcga cgccgaaggc ggcctacggg atcgtggcgg gcatggagct
      721 cgactggtcc ggcgccaccg tctggttcgg ggacgagcgc tgcgtgccgg cggacgacga
      781 gctgtcgaac tacgggatgg ccagggaggc gctgctcgac cgggtcgagg gagcgacggt
      841 gaagcggatg gcgggggaga agggcccgca cgcgggggcg gaggactacg agcgcgagct
      901 gcgcgagacg ctgggcgagg ccctgccgcg gctcgacctg atcctgctcg gcctcggccc
      961 cgaccggcac accgcgtcgc tggttccggg caagccggcc atcgaggtca aggaccgcgc
     1021 ggtggtcggc gtggaggagg ccggcatgcc gccggaggtc ccccgcatca cgctgaccct
     1081 cccggcgatc aacgccgcgc gccaggtcgt gttcctcgtc gccggcgagg acaagtcgga
     1141 tgcggtgcag gccgccttcg gcaacggcga gggaccggcc ggtctcgtag acgcggacgt
     1201 cgtgctcctc gacgagggcg ccgcgagccg gctgacgcgg tgaccacgga cgcgggcgca
     1261 ccgctcacgc ggccggaggt cgaggcctac gacgggcaga gcgcgttccc gccgatcgcc
     1321 gactacgcgt tcctgtcgga ctgcgagacc gtggcgctgg tcgcgccgag cgggaacatc
     1381 gagtggttct gcctgccgcg gccggacggg ccgagcgtgt tcggcgcgct gctcgatcgc
     1441 gacgcgggcg ggttccgcct cggtccggcg gacatgacgg tcccggccgg gcgccgctac
     1501 atccccggca cgaacgtcct cgagacgacc tggatgacgc gcatgggctg gctcgtcgtg
     1561 cgcgacgcgc tctcggtggg tccgtggcac gacgtggacg agcgctccca cacgcaccgg
     1621 cgcacgccga ccgaccacga cgccgagcac gtgctgatcc ggaccgtgca ctgcgtgcag
     1681 gggacggtcg agctgcacct cgactgcgag cccgcgttcg actacggacg tgccggagcg
     1741 gagtggaggt atgccgggga caactacaac gaggccgtgg cgagcgccga cggggtggac
     1801 gtggagctgc ggctcaccac cgacctgcgc gtgggcttcg agggccgccg cgcgcgggcg
     1861 cggcacacgc tgcgcgaggg cgagtcggcg tatgcggcgc tgtcctggag caagcacgcg
     1921 ccgccgaagg acttccagga ggcggtcgac cgcatgatgc ggaccgagga cttctggcgc
     1981 cagtggctca accgcgggct gttccccgat cacccgtggc ggctctacct ccagcgcagc
     2041 gcgctcacgc tgaaggggct cacctatgcg cccacgggcg ccatgctcgc ggccgccacg
     2101 acgtcgcttc cggagacccc gggcggcgag cgcaactggg actaccgcta ctcgtggatc
     2161 cgcgactcca cgttcatgct ctggggcctc tacacgctcg gcttcgactg ggaggccaac
     2221 gacttcttct acttcgtcgc ggacgtggcc ggggacgagg ggaaggacct ccagatcatg
     2281 tatgggatcg ggggcgaggc cgagctggag gaggaggagc tcgaccacct gacgggctac
     2341 gacggcgcca agccggtgcg ggtcggcaac ggcgcgtaca cgcaggccca gcacgacgtg
     2401 tggggcgcgg tgctcgactc ggtctacctg cacacccgct cgcgcgacct gctgccggag
     2461 cggatctggc cgatgctcgt gcaccaggtc gaggcggcgc tcgagaactg gcgcaagccc
     2521 gaccgcggca tctgggaggt gcgcggggat cccaagcact tcgtctcctc gaagctcatg
     2581 tgctgggtgg cggccgaccg tggggcgcgc ctggccgcga tccgcgagga cgacgaggcg
     2641 gccgagcgct gggccgctgc cgccgaggag atcaaggacg acatctgcga gaacggggtg
     2701 gacaagcgcg gcgtgttcac ccagcactac gagaccgacg cgctcgacgc ctccgtgctg
     2761 ctgatgccgc tcgtgcgctt cctgccgccg gacgacgagc gcatcgtgga cacggtgagg
     2821 gcggtcgccg aggagctgac cgagaacggg ctcgtgctgc gctaccgggt cgacgagacc
     2881 gacgatgggc tcgccggcga ggagggcacg ttcgcgatct gctcgttctg gctcgtgtcc
     2941 gccctcgccg agatcggcga ccacaagagc gcccggcagc tatgcgagcg gatgctctcg
     3001 tacgccagtt cgctacagct ctacgccgag gagatagacg ccgtcaccgg gcgccacctc
     3061 ggcaacttcc cgcaggcgtt cacgcacctg gccctgatca acgcggtgat gcacgtgatc
     3121 cgcgcggacc aggacgagag cacgcggcgc ttctcgcggg cgcgctaggg cgccgggtca
     3181 cggctggcgc ccgccgcggc gcgttcagcg cccggccgca ccgggaaagg gctggatatg
     3241 gcagggatgc ccggcagggt cactcgatcc gtggtgttcg tgatcgcggc cgtagcgctg
     3301 ctcgcgttcg gtgtcgcgcg ggcggggagt gtcgaggtgg tcagcagcgc cgtgccggag
     3361 ctccaggcgc cggcttcgtt gccctcggcg ccgagcgtgc ccagtgtgcc ggctccgagc
     3421 gtgccgagcg cgccgagcgt tccgtctgcg ccctccgtgc cgagcgcgcc ctcggctccg
     3481 agcgcaccct ccgtgcctgg cgtgaccgtg ccgggtgctg gctcgggcag cggttctgga
     3541 tcgggctcgg gctcgggctc gggctcgggc tcgggtgctg gctctggctc cgcctcgggt
     3601 gccggctccg gttccgccgc ggggtccggg tccgctgacg gcggcggggg aggggagggg
     3661 cccggttccg ggtctggctc cacggacggc tccggttcgg ccggcggatc cggctccggc
     3721 gaacgctccg gttcctccgc atccccgcgc gtgcgcgctg cgcgtgcgcg ggcgcgcgag
     3781 gccgcggcgc gcgagcggcg cgtccggcgc gagcggcggc tgcgcgccct ggtcacgcgg
     3841 ctcaccggct gcctcgactt cctgccggag tccgagggcc gctacctcgc gctgcgcgcg
     3901 ggcgtcggct cggagcggcc gcagtcgcgc cgcgcggccg cgcggcaggc cgggatcccc
     3961 gcaggccgtg cggcgacgac cgagcgccgc ggcctgaggc gcctgcgccg cgccgcgcgc
     4021 gccggcgggt gcggtgccgg ctccgccggc gccagcgtgg ccctgacggg cgcgaccggg
     4081 ccccagctcc agccggccgt gctgatggcg cccgcaccgg cactgcgcag cgcggtcgag
     4141 atggcgggcg gcggcgagga gcgcgaccgc cagggcgtga aggccgcgac cgcgtcgtcc
     4201 gagtcggcgg agggtgccgg cggagccggt gacggcgacg agcagcgctc ggtgctggcg
     4261 ccgctggccg actccgtgga cgagccctcg gccaccggct ggtacgtcct cctggccgcc
     4321 gtcgcggcgc tctcggtggc cctcctaatt ctccagcgcc gcgaccgcgc agcccac
//
LOCUS       DASVPL010000279         9372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_149585, whole genome shotgun sequence.
ACCESSION   DASVPL010000279 DASVPL010000000
VERSION     DASVPL010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149585"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VF517_15510"
     CDS             complement(<1..242)
                     /locus_tag="VF517_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8054395.1"
                     /translation="MSARRSAIVERDGAECVWCGRAPWPRDLTLEHLLPRSRGGRATP
                     ANLTPACRSCNRARRSRPVAAYVRELMAAGRAPRLR"
     gene            300..950
                     /locus_tag="VF517_15515"
     CDS             300..950
                     /locus_tag="VF517_15515"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX8054396.1"
                     /translation="MSPDQSVIDSPVREFLDELASKAPTPGGGSAAALGAALGAGLVS
                     MVCQFTVGREKYAEVDADMRRVLERSEALRAELQRAVDADVAAYGSYSTASGMPRETD
                     DEKRERAAALQAALRESTEVPLGVAERCAELLELAVEAAEKGNVYLISDAAVGAEMAA
                     AARASAELNVRLNAGGLEDRDFARASEERLGAIERRAPGRELAERATRVVEERNAG"
     gene            947..1777
                     /locus_tag="VF517_15520"
     CDS             947..1777
                     /locus_tag="VF517_15520"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX8054397.1"
                     /translation="MSARVLDGRTVAAELKEELRAAAAEAGGDAGLAIVRSADDAAAV
                     YAGRLEKLCAELGVPSRSAELEGDTAGAVATVEELGRDPSIAGILVQLPLPDGVDQDA
                     VVAAVDPAKDVDGVHPLNAGRLFLGLPALAPATPLAVMELLRRHDVEVAGRRAVVVGR
                     SNITGKPVAMLLLHANATVSVCHSRTPDLGAAVREGEIVVACAGRAGLIGGEMIAPGA
                     VVVDVGTNFVDGKLVGDVRYDEVAEVASAASPVPGGVGPLTNLMLVRNLLDAAKRARR
                     "
     gene            complement(1758..2714)
                     /locus_tag="VF517_15525"
     CDS             complement(1758..2714)
                     /locus_tag="VF517_15525"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEX8054398.1"
                     /translation="MPPAHETAEILFEGYPARALISHAAGVEAVFTPKIGMLGCSLTL
                     DGEELLGQRGGLARYEATGSTMGIPLLHPWANRLAGVSYTAAGRTVELPAGSPLIRTD
                     PNGLPIHGVVNASPYWEVMAAGADDTTAGLSARLDFGAHPELLEAFPFPHELRIDVTL
                     RDATLAVRTVLTATGEDPVPIAFGYHPYLALPGVPRADWHVEVPASRRLILDERMIPT
                     GATEEVEPYDGPLGDRAFDDGYAGIEDGTRFALAGGGRRIELELADGYPFAQVYAPAG
                     EELIAFEPMTAPTNALASGQDLRLAEPGTPRSATFAISVARA"
     gene            complement(2720..3418)
                     /locus_tag="VF517_15530"
     CDS             complement(2720..3418)
                     /locus_tag="VF517_15530"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8054399.1"
                     /translation="MTQPERGVKASREPNFLLAKVEKRALQTIARRLPRRVLPDDLTA
                     LGVAAALGICAAYQLTNESKAWLWVASALLVVHWFGDSLDGTLARVRKIERPRYGYYL
                     DHLVDAISTALIGIGLGLSPYLLLAVGTLIVVAYLTLSINVYLESQAFGRFSIGYGFV
                     GPTEVRLILIALNTLLALGIGLDFTLVDLHMTVFDIVGLAIAGTMIALLLGRGVRNLR
                     ELAREEPAGSRRNP"
     gene            complement(3543..3767)
                     /locus_tag="VF517_15535"
     CDS             complement(3543..3767)
                     /locus_tag="VF517_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054400.1"
                     /translation="MLLAFLVGLAMVIAAGAGLAMLIDPETFLAWSGSNCGDTGRCSN
                     TDLRILGGILAFVVGPAGIALFLRAAPWRR"
     gene            3832..3905
                     /locus_tag="VF517_15540"
     tRNA            3832..3905
                     /locus_tag="VF517_15540"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3866..3868,aa:Gly,seq:tcc)
     gene            4425..4498
                     /locus_tag="VF517_15545"
     tRNA            4425..4498
                     /locus_tag="VF517_15545"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4459..4461,aa:Pro,seq:ggg)
     gene            4719..5063
                     /locus_tag="VF517_15550"
     CDS             4719..5063
                     /locus_tag="VF517_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054401.1"
                     /translation="MSLWRRLWADDQARPEGLSDRQRLEQLLYRLADDIADDDHAISR
                     LSWSREVRAAAGLVGAGNPRGLSDFVGLFDPDPRNTFNEQRFVNTRTFDEAGPARVQA
                     VVRARRGGAPQT"
     gene            complement(5353..6207)
                     /locus_tag="VF517_15555"
     CDS             complement(5353..6207)
                     /locus_tag="VF517_15555"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8054402.1"
                     /translation="MAELCVHIDEFTDPLCPWAWSAEPFRRRLRWLYGEAISWAPRMV
                     VLADEASEQVERGFTPERLSEASKRIAREHRMPIDTAVRDYVPGSGPACRAIVAAREH
                     GEPHEGRALLRELRVHYFRGEQIDRPEVIESAARAARIDPGELDRWIADPATKAALAA
                     DRAAAREPKPAARVLDEKLANWSGGRRYTCPSYEITRIEDGVTISVPGFQPFAVYNTI
                     LANLVPGLERRELPGDVSEVLDWSAIPISTQEVAVMCDIDFDEARERLGRVARHEPVG
                     ADGFWTRA"
     gene            6360..6854
                     /locus_tag="VF517_15560"
     CDS             6360..6854
                     /locus_tag="VF517_15560"
                     /inference="COORDINATES: protein motif:HMM:NF023056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HEX8054403.1"
                     /translation="MSDERTADGRYILVGGRRWRATDPTIPEDRRQELVDELMAARRA
                     VAEAKRADDAGAERAARARVHAAKVALGERGPKWWERIEREVLEMVAARGPGRTICPS
                     EVARSLSGDDEVRPLMPHVREAAAQLAERGEIAVTQKGERVDARSAKGPIRLGLINGG
                     EASH"
     gene            6906..7868
                     /locus_tag="VF517_15565"
     CDS             6906..7868
                     /locus_tag="VF517_15565"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8054404.1"
                     /translation="MTGDPPPRPSPTAVVATARTLGLDALTVEVLRAFRTAGCRSVLL
                     KGQSFRRHLYADGAPRSYGDVDLLVAPTDLARAGEVLASLGFAKGLDHSHHPGLVEPH
                     AQEWGRPGGPRVVDLHWRIAGVGAPAERSWEVLSACTELMPVAGEPAESLRAEGVALL
                     VALHAASHGPTVEKVVRDLERAVERLDLETWTAAARLAAELDATEAFASGLRVVPTGD
                     SLANELNLPGVRSPQRVLLDGGSPAGSHGLLRIVQPAPLRERLRALRYALIPPPIYMR
                     ATSSLAARGRVGLVLAYLARAGLRTLQLPAAIRAVRGSRAASRP"
     gene            complement(7858..8841)
                     /locus_tag="VF517_15570"
     CDS             complement(7858..8841)
                     /locus_tag="VF517_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054405.1"
                     /translation="MTHRYEAFGLSIRSNHPLQGLHASETRQIDVDIAFDASGAPEVD
                     RRAADTTSHTGSGSVHECEDGGRMLLYASHGGERAWSLRVSGDGRAIEVRWRGPIELA
                     DVAAFVETTGLPTALLLRAVPLLHGCAVDTGRAAFLVLGPGGAGKSTVAAAAVAGGHA
                     LLTDDIAALDTTGDHVRVHPGGSQLRMNADTAEALGWAPAELRRVFATPTMPPKLYAR
                     LSRADGSLCDGARRVAAIFVLGARRSRSVRIERIAPSAALPVILRNTFGERAVGARTR
                     AHLLPVWARLAREVPVHTVDPPDGLALAPSLVEALVAETAAAGGRLDGLRA"
     gene            complement(8838..>9372)
                     /locus_tag="VF517_15575"
     CDS             complement(8838..>9372)
                     /locus_tag="VF517_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054406.1"
                     /translation="SDAVAERLAARLGEASRQEPEAALSTCRELVALHPAYDQAHLLA
                     ARCLERLDRHEEALAYAERVDPSPAYSDLFAELRARAAARLGRPDRARHADAIRSPFR
                     RARLGAFASPAEQSHAVLEHNPYDHFALASTIRRIERNDADAPELGPLYERLCMVAPE
                     DVPPARRPARLAPFLPA"
BASE COUNT         1292 a         3219 c         3590 g         1271 t
ORIGIN      
        1 tcgcgcagac gcggcgcgcg ccccgccgcc atgagctcgc ggacgtacgc ggcgaccggc
       61 cgggagcggc gggctcggtt gcacgagcga caggcggggg tcaggttcgc cggtgtcgcg
      121 cggccgccgc gcgagcgggg gaggaggtgc tcgagggtga ggtcgcgcgg ccacggcgct
      181 cgcccgcacc agacgcactc ggcaccgtct cgctcgacga tcgctgacct gcgcgcgctc
      241 accgtccgga cgataagagg ccggcttcgg ccgacggccg tgttatacgt cccggccccg
      301 tgagccccga tcagagcgtc atcgattccc ccgtccgcga gttcctcgac gagctcgcgt
      361 cgaaggcccc cacgcccggc ggcggcagcg cggcggcgct gggcgcggcg ctcggggcgg
      421 ggctcgtcag catggtctgc cagttcacgg tcggtcgcga gaagtacgcc gaggtggacg
      481 cggacatgcg gcgcgtgctg gagcgctcgg aggcgctgcg ggcggagctc cagcgggcgg
      541 tcgacgcgga tgtggccgcc tacgggagct actcgaccgc gtcggggatg ccgcgcgaga
      601 ccgacgacga gaagcgcgag cgggcggcgg ccctgcaggc ggcgctgcgc gagagcaccg
      661 aggtgccgct gggcgtggcg gagcgctgcg cggagctgct cgagctggcg gtggaagcgg
      721 cggagaaggg caacgtgtac ctgatcagcg acgccgccgt gggggccgag atggccgccg
      781 ccgcgcgggc gtcggcggag ctcaacgtgc ggcttaacgc gggcgggctc gaggaccgtg
      841 acttcgcgcg cgcgagcgag gagcggttgg gtgcgatcga gcggcgggcg cccgggcgcg
      901 agctggcgga acgcgcgacg cgggtggtcg aggagcgcaa cgccggatga gcgcgcgcgt
      961 gctcgacggc aggacggtcg ccgcggagct gaaggaggag ctccgcgcgg ccgcggcaga
     1021 ggccggcggc gacgccgggc tggcgatcgt gcgcagcgcc gacgacgccg ccgccgtcta
     1081 cgccgggcgg ctcgagaagc tgtgcgcgga gctcggggtg ccgtcgcgct cggcggagct
     1141 ggagggcgat acggccggcg cggtcgcgac cgtggaggag ctcgggcgcg atccgtcgat
     1201 cgccgggatt ctcgtgcagc tcccgctgcc ggacggggtg gaccaggacg cggtggtcgc
     1261 ggcggtggac ccggccaagg acgtggacgg cgtgcacccg ctaaacgcgg gccggctctt
     1321 cctcgggttg ccggcgctgg ctcccgccac tccgctggcg gtgatggagc tgctgcgacg
     1381 ccacgacgtc gaggtggccg gcaggcgcgc cgtcgtggtc gggcgcagca acatcacggg
     1441 caagccggtg gcgatgctgc tgctgcacgc gaacgcaacg gtcagcgtgt gccattcgcg
     1501 tacgccggac ctcggcgccg ccgtgcgcga gggcgagatc gtggtggcgt gcgcggggcg
     1561 ggccggcctg atcggcggcg agatgatcgc gcccggagcg gtggtggtgg acgtgggcac
     1621 caacttcgtc gacggcaagc tcgtgggcga cgtgcgctac gacgaggtgg ccgaggtggc
     1681 gtcggcggcc tcgccggtgc cgggcggggt ggggccgctc acgaacctga tgctcgtccg
     1741 caacctgctc gacgcggcta agcgcgcgcg acgctgatcg cgaacgtggc cgagcgcggg
     1801 gttcccggct cggcgagccg gaggtcctgg ccgctcgcga gcgcgttcgt tggagcggtc
     1861 atcggctcga acgcgatcag ctcctcgccc gccggcgcgt agacctgggc gaacgggtag
     1921 ccgtcggcga gctcgagctc gatgcgcctg ccgccgccgg ccagggcgaa gcgggtgccg
     1981 tcctcgatcc cggcgtagcc gtcgtcgaag gcgcggtccc cgagcgggcc gtcgtagggc
     2041 tccacctcct ccgtcgcgcc cgtcgggatc atgcgctcgt cgaggatgag ccgccggctc
     2101 gcgggcacct ctacgtgcca gtcggcgcgc gggacgcccg gcagcgcgag gtatgggtgg
     2161 tacccgaacg cgatcgggac cgggtcctcg cccgtggcgg tgagcaccgt gcggacggcc
     2221 agggtggcgt cgcgcagggt cacgtcgatg cgcagctcgt gcgggaaggg aaacgcctcg
     2281 agcagctcgg gatgggcgcc gaagtcgagc cgcgcggaga gaccggcggt cgtgtcgtcg
     2341 gcaccggcgg ccatcacctc ccagtacggc gaggcgttca ccacgccgtg gatcggcagc
     2401 ccgttcgggt cggtgcggat cagcggcgag cccgcgggca gctcgacggt gcggccggcg
     2461 gccgtgtagg agacgccagc gaggcggttc gcccacgggt gcaggagcgg gatccccatg
     2521 gtcgagccgg tggcctcgta gcgggcgagg ccgccgcgct gcccgagcag ctcctcgccg
     2581 tcgagggtga gcgagcagcc gagcatcccg atctttggcg tgaagaccgc ctcgacgccc
     2641 gcggcgtgcg agatcagggc gcgggccggg tagccctcga agaggatctc tgcggtctcg
     2701 tgggcgggcg gcactcgcct caggggtttc ggcgcgagcc ggctggctcc tcgcgcgcga
     2761 gctcgcgcag gttgcggacg ccgcggccga gcaggagcgc gatcatcgtg cccgcgatgg
     2821 cgaggccgac gatgtcgaag accgtcatgt ggaggtccac gagcgtgaag tcgagcccga
     2881 tgcccagcgc gagcagcgtg ttcagagcga tcaggatgag gcggacctcg gtggggccta
     2941 cgaatccgta gccgatgctg aagcgaccga acgcctggct ctcgaggtag acgttgatcg
     3001 acagcgtgag gtaggcgacg acgatcagcg tcccgacggc cagcagcagg tagggggaga
     3061 ggccgaggcc gatgccgatc agggcggtcg agatcgcgtc gaccaggtgg tcgaggtagt
     3121 agccgtagcg cgggcgctcg atcttgcgga cgcgggcgag ggtgccgtcg aggctgtcgc
     3181 cgaaccagtg gacgaccagc agggcgctcg ccacccagag ccaggccttc gactcgttcg
     3241 tgagctggta ggcggcgcag atgccgagcg cggcggcgac gccgagcgcg gtgaggtcgt
     3301 cggggaggac gcggcggggg aggcggcggg caatggtctg aagggcgcgc ttctcgacct
     3361 tcgcgaggag gaagttgggc tcgcggctgg ccttgacgcc gcgctcgggc tgggtcactg
     3421 tcgcgcccgc tcggtctccg cctccacgcc ttcggtcccc gcgccggtgc ctccggtccc
     3481 cgcctccacg ggttggttgt accaaggctt ggggagaagg tgacgtgcgt cagcggacgg
     3541 ggtcagcgcc gccagggcgc ggcgcgcagg aacagcgcga tgccggccgg gccgacgacg
     3601 aaggcgagga tgccgccgag gatccgcagg tcggtgttgc tgcaccggcc cgtgtcgccg
     3661 cagttcgacc cgctccaggc gaggaaggtc tcggggtcga tcagcatcgc cagtccggcg
     3721 ccggcggcga tgaccatggc gagtcccacg aggaacgcga ggagcatggc gcgagcgtac
     3781 tctcgccggg gcgccgcggc ggggccggaa ctagactggc ggcgtagcca cgcgggcgtg
     3841 gtgtagttgg tttgcacgcg agccttccaa gctcgaggtc acgggttcga atcccgtcgc
     3901 ccgctcttct gattccagcc tcatcgccct tgcgcgcagc ccatccccgc gatgtcggcg
     3961 ccggtggcag ttcgcgcaga cgacgtcgca cttctcgatc tcggccaaca cggacgccca
     4021 cgcatgcgtc gcgagcttgg tcccgatgtt gaacgccttc gtgctcggat ccacgtgatc
     4081 gaactcgaga acgatcgggt cgctctcccc gcaatcggca cagggatgcg acttgaagaa
     4141 ctcgagtagg taacgcgtgc gttccaacgc gatccgccgc ttcgcgttcg cgacgcgctc
     4201 ggcataccgc tgcctgttgg ccaagtagta cttgcggctg taggcgaccc ggcagggacg
     4261 gcagaacgag tcacggcggc ccttcgcctt caagcgccaa gcgaagtgtt caggcggctt
     4321 cagttcgccg cacatggagc acttccgtag cgggtcgtcc gcgtgcacga acacatgttc
     4381 gcatatcacc cggacggatc cgcggctaag atccggcgca gccacggggc gtggcgcagt
     4441 ctggtagcgc attcggctgg gggccgaaag gtcgctggtt caaatccagt cgccccgatt
     4501 gacgcggaaa gcctcccagg ggcggctttc cgctttcaag ggaactcgct ctttcaaacc
     4561 ggatggccgg cagcgccggc tttcgcccgt ccccacccgc ctgcattacc ccccggcgcg
     4621 ggccacgact cctgctttgc atgtacgcga ttcggccccc gcgtactggt catcatggga
     4681 tgctggctgg catgcgaacg ctctgagctg gaccggcgat gagcttgtgg cgccggctgt
     4741 gggccgacga tcaggcgcgg cccgagggat tgtcggacag gcagcggctc gagcagttgc
     4801 tctaccgact agccgacgac atcgccgacg atgaccacgc catcagtcgc ctttcctggt
     4861 cgcgcgaggt gcgcgcagcc gcgggtctgg tcggtgccgg aaatccccgg ggtctcagcg
     4921 acttcgtcgg cctattcgac cccgacccca ggaacacttt caacgagcag cgcttcgtca
     4981 acacgagaac ttttgacgag gcgggcccgg ctcgcgtgca ggctgttgtc cgagcacgaa
     5041 gaggaggagc gcctcagacg tgaggccgag gaccaggttg tccttcgtcc gtggagagag
     5101 ggcagtaccg gcaaggtggt ggtctacgcg gacgggacgg tcattacgag cgaagacgat
     5161 gccggtggag aaccccaatt cgaaagcatc aaggatgcgc gtcagcgtcg ggccccagtc
     5221 gcgctgatcg ccatcgaagc ggacggctca tgcgacgcac acaccgcaac ctccgagacg
     5281 agcctggctc gctgcccggc tgcacgacca cgacccactg ttgcacctgg gaccaagccc
     5341 tcgggggagc gcctaggcgc gggtccagaa cccgtcggcc ccgacgggct cgtgcctggc
     5401 gacgcggccg agccgctcgc gagcctcgtc gaagtcgatg tcgcacatca ccgccacctc
     5461 ctgcgtggag atcgggatgg ccgaccagtc gagcacctcg ctgacgtcgc cgggcagctc
     5521 acggcgctct aggccgggaa cgaggttcgc gaggatcgtg ttgtagacgg cgaacggctg
     5581 gaagcccggg accgagatgg tcacgccgtc ctcgatcctc gtgatctcgt agctggggca
     5641 cgtgtagcgg cggccgcccg accagttggc gagcttctcg tcgagcacgc gtgcggcggg
     5701 cttcggctcg cgggcggcgg ccctgtcggc cgcgagtgcg gccttggtcg ccgggtcggc
     5761 aatccagcgg tcgagctcgc cggggtcgat ccgcgccgct cgcgctgctg actcgatcac
     5821 ctcggggcgg tcgatctgct caccacggaa gtaatgcacc cgcagctcgc gcagcagtgc
     5881 gcggccctcg tgcggctcgc cgtgctcgcg ggcggccacg atcgcgcggc acgccggccc
     5941 gctgcccggc acgtagtcgc gtaccgccgt gtctatcggc atgcggtgct cgcgggcgat
     6001 ccgcttcgac gcctcggaga gccgctccgg cgtgaagccg cgctccacct gctcggacgc
     6061 ctcgtccgcc aggaccacca tccgcggagc ccagctgatc gcctcgccgt agagccagcg
     6121 caggcgacgc cggaatggct ccgcgctcca cgcccacggg cagagagggt cggtgaactc
     6181 gtcgatgtgg acgcacagct cggccatggg gcgagtgtgg cagagggtgt ggcgacacgc
     6241 ccgggacgcc gccgtgaggc gcgttctact cgcggcgata gcgagtgcag cgtcgcgctg
     6301 agctcgcgcg acctggtgga gtgctagagc caccacggcc gttcactacg gtcagacggg
     6361 tgagtgacga gcggacggct gacggccggt acatcctcgt cggcggccgc cgctggcgcg
     6421 cgaccgaccc gacgatccca gaagatcgtc gccaggagtt ggtcgacgag ctgatggctg
     6481 cgcggcgggc cgtggccgag gcgaagcgcg ccgacgacgc cggggcggag cgagccgccc
     6541 gggctcgcgt gcacgccgcg aaggtggcgc tcggcgagcg aggtccgaag tggtgggagc
     6601 gcatcgagcg cgaggtgctc gagatggtcg ccgcgcgcgg gccgggcagg accatctgcc
     6661 cctcggaggt cgctcggtcc ctgtccggcg atgacgaggt ccgtccgctg atgccgcacg
     6721 tgcgcgaggc ggcggcgcag ctcgcggagc gaggggagat cgccgtgact cagaaggggg
     6781 agagggtcga cgcgcggtcc gccaagggtc cgattcgcct cggcctcatc aacggcggcg
     6841 aggcgagcca ctgagcccca tcaccgccgc gcgcgccggc gagcgccgcg tagagtgagc
     6901 cgcccgtgac cggcgacccg ccgccccgcc catccccgac ggcggtcgtc gcgaccgccc
     6961 ggaccctcgg gctcgacgcg ctcaccgtcg aggtgctgcg tgccttccgc accgcggggt
     7021 gccgcagcgt ccttctcaag gggcagtcgt tcagacgcca cctgtacgcc gacggagcac
     7081 cgcgcagcta cggggacgtc gacctactcg tcgccccgac cgacctcgca cgggccggag
     7141 aggtgctggc gtcgctcggc ttcgcgaagg ggctcgatca cagccaccat ccgggtctgg
     7201 tcgagccgca cgcgcaggag tgggggcggc cgggcggccc gcgcgtggtg gacctgcact
     7261 ggcggatcgc cggagtcggg gcgcccgcgg agcgctcgtg ggaggtcctc tcggcgtgca
     7321 ccgagctgat gccggtcgcc ggcgagccgg ccgagtctct ccgtgcggaa ggcgtcgccc
     7381 tcctggtcgc gctccacgcg gccagccacg gcccgacggt cgagaaggtg gttcgagacc
     7441 tcgagcgtgc cgtcgagagg ctggacctcg agacatggac cgccgccgcg cgtctggccg
     7501 ccgagctcga cgcgaccgag gcgttcgcct ccggactgcg agtcgtgccc accggcgact
     7561 cgctggcgaa cgagctgaac cttccagggg tgcggtcgcc gcagcgcgtg ctgctcgacg
     7621 gcggctcgcc cgccggctcg cacggcctgt tgaggatcgt gcaacccgcg ccgttgcgtg
     7681 agcggctgcg ggcgctgcgc tacgcgctga tccccccgcc catctacatg cgggcgacgt
     7741 cgtcgctggc cgctcgcggc cgggtgggcc tcgtgctcgc ctacctcgcg cgggcggggc
     7801 tgcggaccct ccagctgccc gcggcgatcc gcgccgtccg ggggtcccgc gccgcctcta
     7861 ggccctgagg ccgtccagcc gaccgccggc cgccgccgtc tcggcgacaa gcgcttcgac
     7921 gagcgacgga gccagggcga ggccatccgg cgggtccacc gtgtggacgg gcacctctcg
     7981 cgccagccgc gcccacaccg gcagcaggtg cgcacgcgtc cgcgcgccga ccgcgcgctc
     8041 gccgaaggtg ttgcgaagga tcacggggag cgccgcggac ggcgcgatcc gctcgatcct
     8101 caccgatcgc gagcgccgcg cgccgaggac gaagatcgcc gcgacgcgcc gcgcgccgtc
     8161 acagaggctc ccgtccgcgc gggagagccg cgcgtagagc ttcggcggca tggtgggggt
     8221 cgcgaacacc cggcgtagct cggccggcgc ccatcccagc gcctcggccg tgtccgcgtt
     8281 catccgcagc tggctgccac ccgggtgcac gcgcacgtgg tcgcccgtcg tgtcgagcgc
     8341 cgcgatgtcg tccgtcagca gagcgtgtcc cccggcgacg gccgcggccg cgaccgtcga
     8401 cttgccggcg cctccgggtc cgagcacgag gaacgcggcg cgacccgtgt ccaccgcgca
     8461 cccgtggagc agcgggacgg cgcgcagcag aagggccgtg ggcaggcccg tggtctccac
     8521 gaaggcggcg acgtccgcga gctcgatcgg cccgcgccag cgcacctcga tcgcgcggcc
     8581 gtctccactc acgcgcaggc tccacgcgcg ttcgccacca tgcgatgcgt acaggagcat
     8641 ccggccgccg tcctcgcact cgtgtaccga tcccgacccc gtgtgcgacg tcgtgtccgc
     8701 ggctcgccgg tccacctcag gcgcgcccga cgcgtcgaac gcgatgtcga cgtcgatctg
     8761 gcgtgtctcg ctcgcgtgca ggccctggag cgggtggttc gaccggatcg agagcccgaa
     8821 tgcctcgtaa cggtgggtca tgccgggagg aacggcgcga gtcgagcggg ccgtcgggca
     8881 ggggggacgt cctccggggc gaccatgcac aggcgctcgt agagcgggcc gagctccggc
     8941 gcgtccgcat cgttgcgctc gatgcgcctg atcgtgctcg ccagcgcgaa gtggtcgtac
     9001 gggttgtgct cgagcacggc gtgactctgc tcggccgggg acgcgaacgc gccgagccgg
     9061 gccctcctga agggcgatcg gatcgcgtcg gcgtgacgtg ctcggtcggg acgcccgagg
     9121 cgcgccgcgg cgcgcgccct cagctccgcg aacaggtcgg agtaggcggg cgacggatcg
     9181 acgcgctcgg cgtacgcgag cgcctcctcg tgccgatcga ggcgctccag gcagcgcgcc
     9241 gccagcaggt gcgcctggtc gtaggccgga tggagggcca cgagctcgcg gcaggtcgac
     9301 agcgccgctt ccggctcctg acgagaggcc tcgccgaggc gcgcggcgag gcgctcggcg
     9361 acggcgtcgg ag
//
LOCUS       DASVPL010000280         9119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_307408, whole genome shotgun sequence.
ACCESSION   DASVPL010000280 DASVPL010000000
VERSION     DASVPL010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9119
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307408"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VF517_15580"
     CDS             complement(<1..474)
                     /locus_tag="VF517_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054407.1"
                     /translation="MSPARPIRRTLVAALAVAALAPACAAAAEPVPARFFGVVAEPDR
                     LHDRELGPAGTNLEGELDAMRGAGVGSMRMSFFWARIQPYRSWEDIPPDGRHDFTDVD
                     GRPYAFAETDRLVAGAAARGIEVLPVLLWSPAWAARYRNEFASPPKDPRAFAEFAA"
     gene            710..1459
                     /locus_tag="VF517_15585"
     CDS             710..1459
                     /locus_tag="VF517_15585"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8054408.1"
                     /translation="MDLGIDGRVALVTGASKGIGRGIAAALAAEGVRVAVSSRSRERI
                     DAAAAEIGATGFVHDSSDLDAVPRLVDAVEAELGPVDILVANTGGPPGGPDPLGFPRE
                     QWEAAYRELVLGPMALIERVMPGMRERGFGRVLSVASSSVREPIPPLMLSNAHRPGLV
                     GALKTIARQVARDGVTVNSILPGRIATDRIAENFGSLENAEAAARETIAAGRLGTVEE
                     FAAVAAFLCSARASYVTGASVLVDGGLTQSV"
     gene            1463..2653
                     /locus_tag="VF517_15590"
     CDS             1463..2653
                     /locus_tag="VF517_15590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8054409.1"
                     /translation="MPPGRRVPVQLWCDEFPVASETFVAAEAEELARLGHPVEVVASR
                     RPERPAAAAPAGVPVHYLEDDSTATRLLAAAALPLRHPRAVVRDRRARRRWRVDERVA
                     PLARIAPALLRGGGVAHAHFAAEAALGAMRGARVTGRPWSLTAHAYDIYDEPRNLAEK
                     LRSAALVTSGCDYTVRDLRAIAGPEHAERVVRVVMGVDPERFRRATPHPAARTVLAVG
                     RLVEKKGFVHLVRAAAEPALAGVVERITIVGEGPLREELEREIATLGLEGVVELAGRR
                     EHGEVRELLESAALVAVPCVVAASGDRDSMPVVAKEALAMEVPVVASDEVGLPELIRP
                     EFGRLVPPGDAPALAAALAELLALAPEERAAMGRAGREHVERHANVHVETARLSELLD
                     SLNT"
     gene            complement(2678..3631)
                     /locus_tag="VF517_15595"
     CDS             complement(2678..3631)
                     /locus_tag="VF517_15595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8054410.1"
                     /translation="MSTDRVAVVVPNFNGERLLPGCLGALRAQTRAPDEVVVVDNGSR
                     DGSRELVATLHPQARVLALARNRGFAGGANAGVGETAAERVAVLNTDARPARDWLERL
                     LAAPAPDHVWAWGSVLVAPGGTRVESAGDHWSDEGYAYKLGHGASVGELPGEPYEIFS
                     PPGAAPLFRRDRFLELGGYEESFFLYFEDLDLSYRALLRGWRALLVPGARVEHDLGAS
                     GRGWARRYYVGRNALRCTVRCVPEPDARAIARRALHELRANPRPALAPFELAGRAAAL
                     ARLPLTLRERRAIQAARTLTPAEVRERLREPAATAGLPRGT"
     gene            complement(3636..4718)
                     /locus_tag="VF517_15600"
     CDS             complement(3636..4718)
                     /locus_tag="VF517_15600"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8054411.1"
                     /translation="MTTAAANEPAGAALETRGEGAFWRDSLAPYARPRLGLSLLDLAT
                     SLVPYLVLSVLMYLALDVSYLLTLAIGIPAAGFLLRTYIMFHDCAHGSFVPGRRANVW
                     LGRLLGVIVYSPYQAWRHNHAVHHATAGDLDRRGVGDLPTMTVAEYRSASRGARIGYR
                     LFRNPLVMFGIGPIYALMIQPRLWSKSDRTRTKRSVIGTNVALVTVIGLLCWAIGWRE
                     FWMVQMPTAWAAGAAGVWLFYVQHQFEDVYWESTGTWDYADAALRGSSYLRLPKVLQF
                     FTGNIGLHHVHHLSARIPNYNLQRAHDDNPIFHEVPQLSLWDGIRSIRLKLWDEERQR
                     LVRWSELPPAPAPVHAGSTVQPSQPT"
     gene            4815..5099
                     /locus_tag="VF517_15605"
     CDS             4815..5099
                     /locus_tag="VF517_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX8054412.1"
                     /translation="MIGFLVAGLVIGFLARLILPGRQKIGIGWTLVLGVVGSLIGGTI
                     ANALRSGDIWELNAVGFIAAVAASVVLLAVGERAGLGSGDRGGPRLGQGR"
     gene            complement(5106..7088)
                     /locus_tag="VF517_15610"
     CDS             complement(5106..7088)
                     /locus_tag="VF517_15610"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX8054413.1"
                     /translation="MVDRREMRWLALALAVDAALVAGDVAMGPLVGTFVLTPILLAVV
                     VGPRVVAIGGSIAVVLAAVAGSWSDEGFDDAYAVRLLIVAAGAALAYFAAVRRCRSDL
                     AARDLEAAAAVGGIVSVTPTAVIGMDETGVVTLFNPAAEAIFGIAAADALGCDLAELV
                     IPPELRAAHRDGLARVRATGESRVVGKPLELDALRGDGTRFPVSLTITRMPGAGAATF
                     AGFIRDISERRRSEIAQQLRARTAELLDTASDYHNTLAEAVRLPVPDLADWCWIDIPD
                     PSGRILTVVDSRAGVRPAHGRPPASAEEAAERNPQGLRRLLDTGDSVLYPQVSGARLA
                     EFAHSAEHTRQMHEHGVTSLIVVPLRAGRRLVGAMALAATDPHRAYTADDLELAQELG
                     QRLGTAIDNARLYEERGTTAATLMASLRPPAIPDLPGWRTAAIYQPAGRTDEVGGDFY
                     DVFATRDDWMLVIGDVVGHGPPAAALTSLARYSIRAAATLTGSGKPALEHLNDQLRQD
                     GRLALLSAACVRLSEDEDSAYATVAIAGHPRPILIRDGEPRLVGRTSLVLGAADAIEV
                     VEDRIEMLPGDCLVLYTDGVIDAAGESDRFGEERLLRALAGAADSPDARMGSLRAALA
                     GFQHGVQRDDIAALAVERTRAGALDATAAGTLRVPS"
     gene            complement(7147..8376)
                     /locus_tag="VF517_15615"
     CDS             complement(7147..8376)
                     /locus_tag="VF517_15615"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8054414.1"
                     /translation="MKPILELLKAERSARRFFLVHAQSSLGTGAGYVALLLLAYERFR
                     SPWAITLILLAEFIPAMLLGPIFGAAADRGSRKRLLIAADVLRATAFLALVLVDGFFA
                     TFALALLAGTGNAMFNPTVMAALPGLVSRERFPAATSLYGTIEELGYMVGPALAGAAF
                     VVLAPEAMLLVNAITFLGSAAVLMTLAFASEEARAKERSQMLASVRDGMVAARRARGT
                     LTLLFSSVSCVAFLGMVNVGELLLAREELGASDTQFSILVTAMGLGVAGGTLLGAEGG
                     APPVLKRRYLWGVLMCAGGLFACGLAPSFAVALPAFTALGVGNGLAVVHERLLLQSTL
                     ADEIMGRVFGLRSALVSFGFGGAFLLAGGAAAVLGPRPLFLLAAAGALVAWLAASFAL
                     RATWNEVAGPEPAAAFA"
     gene            8894..>9119
                     /locus_tag="VF517_15620"
     CDS             8894..>9119
                     /locus_tag="VF517_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054415.1"
                     /translation="MARALAALPEYPVHAQARERLRVAITERSAGAQLAAIVRSDAGL
                     AVAALRAAAGELGDECGAERAVEVLGAERLG"
BASE COUNT         1213 a         3303 c         3523 g         1080 t
ORIGIN      
        1 cgcggcgaac tcggcgaacg cgcgcgggtc cttcggcggc gaggcgaact cgttgcggta
       61 cctcgcggcc caggccggcg accacagcag aaccggcagc acctcgatgc cgcgcgcggc
      121 cgcgcccgcc acgagccggt cggtctcggc gaacgcgtac gggcgcccgt ccacgtcggt
      181 gaagtcgtgg cgcccgtcgg gcgggatgtc ctcccagctg cggtacggct ggatgcgcgc
      241 ccagaagaag ctcatgcgca tgctgccgac gccggcgccg cgcatcgcgt cgagctcgcc
      301 ctcgaggttc gtgcccgccg ggccgagctc gcggtcgtgg aggcggtcgg gttccgcgac
      361 gaccccgaag aagcgagcgg ggaccggctc ggcggcggcc gcgcaggcgg gcgcgagcgc
      421 ggccacggcg agggcggcga cgagcgtgcg gcggataggg cgggctggcg acacgagggg
      481 tcatccgagc ctaggggcga aggccaccat ttgcacgcgg tcgttcgcgc gggtggcccg
      541 cggccggaga gcgcgcgagc cgccgccgga gcccgcgcgt ggcccgcggc cggagagcgc
      601 gtgcgggccg ctgccggaga gcgcgtgcgg gccgctgccg gagagcacgt gcgggccgct
      661 gccggagccc gcgtgcggcc cacggccgga gcccgcgtag gctccccgca tggatcttgg
      721 gatcgacggg cgcgtcgcgc tcgtgaccgg cgcgtcgaag gggatcggac gcgggatcgc
      781 cgcggcgctg gccgccgagg gcgtgcgcgt ggccgtcagc tcgcggtcgc gcgagcggat
      841 cgacgccgcg gccgcggaga tcggcgccac cgggttcgtg cacgacagct ccgacctcga
      901 cgccgtcccg cggttggtcg acgcagtaga ggccgagctc ggcccggtcg acatcctggt
      961 ggccaacacg gggggcccgc cggggggccc ggacccgctc ggcttcccgc gcgagcagtg
     1021 ggaggcggcg taccgcgagc tggtgctggg accgatggcg ctgatcgagc gcgtgatgcc
     1081 gggcatgcgc gagcgcggct tcggccgtgt gctgagcgtg gcgtcgagct ccgtgcgcga
     1141 gccgatcccg ccgctgatgc tctcgaacgc ccaccgcccg ggactggtgg gcgcgctcaa
     1201 gacgatcgcg cgccaggtcg cccgcgacgg cgtgacggtc aactcgatcc tgccgggccg
     1261 gatcgccacc gaccggatcg ccgagaactt cggctcgctc gagaacgcgg aggcggcggc
     1321 tcgcgagacg atcgccgccg ggcggctcgg caccgtggag gagttcgcgg ccgtggcggc
     1381 gttcctctgc tccgcgcgcg cgtcgtacgt cacgggcgcg tcggtgctcg tggacggcgg
     1441 gctcacgcag agcgtctagc cggtgccgcc cggccgccgc gtgcccgtgc agctctggtg
     1501 cgacgagttc cccgtggcga gcgagacgtt cgtcgccgct gaggccgagg agctggcgcg
     1561 gctggggcat ccggtcgagg tggtcgcctc gcggcggccc gagcggcccg ctgccgccgc
     1621 gcccgccggc gtgccggtcc actacctcga ggacgactcg acggccaccc ggctgctggc
     1681 ggcggccgcc ctgccgctgc gccacccgcg cgcggtggtg cgcgaccggc gcgcgcgccg
     1741 gcgctggcgg gtcgacgagc gggtggcgcc gctcgcgcgg atcgcgcccg cgctgctgcg
     1801 cggcggcggg gtcgcgcacg cgcacttcgc cgcggaggcg gcgctcggcg ccatgcgcgg
     1861 cgcgcgcgtg accggccgcc cgtggagcct taccgcccac gcctacgaca tctacgacga
     1921 gccgcgcaac ctcgccgaga agctgcgcag cgcggcgctc gtcacgtcgg ggtgcgacta
     1981 cacggtgcgc gacctgcgcg ccatcgcggg gccggagcac gccgaacggg tggtgcgcgt
     2041 ggtcatgggc gtcgatccgg agcgcttcag gcgcgcgacc ccgcaccccg cggcgcggac
     2101 cgtgctggcg gtgggacgac tggtcgagaa gaagggcttc gtgcacctgg tgcgggcggc
     2161 ggccgagccg gcgctggctg gcgtggtcga gcggatcacg atcgtgggcg aggggccgct
     2221 gcgcgaagag ctcgagcggg agatcgcgac gctggggctc gagggcgtgg tggagctggc
     2281 cgggcggcgc gagcacggcg aggtgcgcga gctgctcgag tcggcggcgc tggtggcggt
     2341 cccgtgcgtg gtggcggcga gcggcgaccg cgactcgatg cccgttgtcg ccaaggaggc
     2401 gctggcgatg gaggtgccgg tggtggcgag cgacgaggtc ggcctccccg agctgatccg
     2461 cccggagttc gggcgcctgg tcccgccggg cgacgcgccc gcactggcgg cggcactggc
     2521 ggagctgctg gcgctggcgc cggaggagcg ggcggcgatg ggccgcgcgg gtcgcgagca
     2581 cgtggaacgc cacgcaaacg tgcacgtgga gacggcgcgg ctgagcgagc tgctcgactc
     2641 tctgaacact taatgacccc atagggtcat taagtgttca agtcccgcgt ggaagccccg
     2701 ccgtggccgc tggctcgcgc aggcgctcgc gcacctcggc gggcgtcagg gtgcgcgcgg
     2761 cctggatcgc ccgccgctcg cgcagggtga gtggcaggcg cgccagcgcc gccgcgcggc
     2821 ccgccagctc gaagggcgcc agcgccggtc gcgggttcgc ccgcagctcg tgcagcgccc
     2881 gccgcgcgat cgcccgcgcg tccggctcgg gcacgcagcg gaccgtgcag cgcagcgcgt
     2941 tgcgccccac gtagtagcgc cgcgcccaac cgcgccccga cgcgccgagg tcgtgctcga
     3001 cccgcgcccc gggcacgagc agcgcgcgcc agccgcgcag cagcgcgcgg taggagaggt
     3061 cgaggtcctc gaagtagagg aagaagctct cctcgtaccc gcccagctcc aggaaccggt
     3121 cgcggcggaa cagcggcgcg gcgccgggcg gcgagaagat ctcgtagggc tcccccggca
     3181 gctcccccac gctcgcgccg tgaccgagct tgtacgcgta gccctcgtcg gaccagtggt
     3241 cgcccgcgct ctccacccgc gtgcccccgg gggcgaccaa caccgagccc caggcccaga
     3301 cgtggtccgg cgcgggtgcc gcgaggagcc gctcgagcca gtcgcgcgcc gggcgcgcgt
     3361 cggtgttcag caccgcgacc cgctccgccg cggtctcccc cacccccgcg ttcgccccgc
     3421 ccgcgaaccc acggttgcgc gccagcgcga gcacgcgcgc ctgcggatgg agcgtggcca
     3481 cgagctcgcg cgagccgtcg cgggagccgt tgtcgaccac cacgacctcg tcgggcgcgc
     3541 gcgtctgcgc gcgcagagcg ccgaggcacc cgggcagcag ccgctcgccg ttgaagttcg
     3601 ggacgacgac cgcgacgcgg tcggtgctca tgcggctagg tcggctggga gggctgcacg
     3661 gtcgagccgg cgtgcaccgg cgcgggcgcg ggcggcagct cggaccagcg cacgagccgc
     3721 tggcgctcct cgtcccagag cttgaggcgg atcgaccgga tcccgtccca caggctgagc
     3781 tgcggcacct cgtggaagat cgggttgtcg tcgtgagcgc gctggaggtt gtagttcggg
     3841 atccgcgcgc tcaggtggtg gacgtggtgg agcccgatgt tgccggtgaa gaactggagc
     3901 accttcggca gcctgaggta ggagctgccg cgcagcgccg cgtcggcgta gtcccacgtc
     3961 cccgtgctct cccagtagac gtcctcgaac tggtgctgga cgtagaacag ccacacgccg
     4021 gccgcgccgg ccgcccaggc cgtgggcatc tgcaccatcc agaactcgcg ccagccgatc
     4081 gcccagcaga gcaggccgat aacggtgacg agcgcgacgt tcgtgccgat cacgctgcgc
     4141 tttgtgcggg tccggtcgct cttcgaccac agccgcggct ggatcatcag cgcgtagatc
     4201 gggccgatcc cgaacatgac gagcgggttg cggaagagcc ggtaaccgat acgcgcaccg
     4261 cgggaggccg accggtactc cgccacggtc atcgtcggga ggtcgcccac gccgcgccgg
     4321 tcgaggtcac cggcggtcgc gtggtgcacg gcgtggttgt ggcgccacgc ctggtagggc
     4381 gagtagacga tcacgccgag caaccgcccg agccacacgt tggcgcgccg gcccggcacg
     4441 aacgatccgt gggcgcagtc gtggaacatg atgtaggtgc gcagcaggaa gccggcggcc
     4501 ggtatgccga tcgcgagcgt gagcaggtac gacacgtcga gcgccaggta catcagcacc
     4561 gacagcacca ggtacgggac gagcgaggtc gcgaggtcga gcaggctcag cccgaggcgc
     4621 ggccgggcgt agggcgccag cgagtcgcgc cagaacgctc cctcgccacg cgtctccaag
     4681 gcggccccgg cgggctcgtt cgcggctgcg gtcgtcatga acggggaacc tctacccggg
     4741 tcgtgggggc caaatctgcg ctggcgcgtg gcgactctcg ggttgaggcc gcctccgccg
     4801 ggtagggcat gtgcatgatc ggtttcctcg ttgccgggtt ggtcatcgga tttctcgctc
     4861 gcctgatcct gccgggccgg cagaagatcg ggatcggctg gaccctagtg cttggcgtag
     4921 tcggctcgct gatcggcggg acgatcgcca atgccctgcg cagcggcgac atctgggagc
     4981 tgaacgccgt cggcttcatc gcggcggtcg ccgcgtcggt cgtcctgctc gccgtcggcg
     5041 agcgcgcggg cctcggctcg ggcgatcgcg gcgggccccg gctcggccag ggtcgctagc
     5101 tccgctcaac tcggcacccg cagggtcccg gccgcggtgg cgtccagcgc tccggcccgg
     5161 gtgcgctcga ccgccagggc cgcgatgtcg tcgcgctgca cgccgtgctg gaagccggcc
     5221 agcgccgcgc gcaggctgcc catgcgggcg tcgggcgagt cggcggcgcc ggcgagcgcc
     5281 cgcaagagcc gctcctcgcc gaagcggtcg gactcgcccg cggcgtcgat caccccgtcg
     5341 gtgtagagca cgaggcagtc gccgggcagc atctcgatcc ggtcctcgac cacctcgatc
     5401 gcgtcggctg cgcccagcac gagcgacgtg cggcccacta ggcgcggctc gccgtcgcgg
     5461 atgaggatcg gccgcgggtg acccgcgatg gcgacggtgg cgtaggcgct gtcctcgtcc
     5521 tcgctcaggc gcacgcacgc ggccgacagc agtgcgagcc gcccgtcctg gcggagctgg
     5581 tcgttcaggt gctcgagcgc cggcttgccg ctgccggtga gggtggccgc agcgcggatg
     5641 ctgtagcggg cgagcgatgt gagcgccgcg gccggcggcc cgtgccccac cacgtcgccg
     5701 atcacgagca tccagtcgtc gcgcgtggcg aagacgtcgt agaagtcgcc gccgacctcg
     5761 tcggtgcggc cggcgggctg gtagatcgcg gcggtcctcc agcccggcag gtccgggatc
     5821 gcgggcgggc gcaggctcgc catcagcgtc gccgcggtgg tcccgcgctc ctcgtagagc
     5881 cgcgcgttgt cgatggccgt gcccagccgc tggccgagct cctgggcgag ctcgaggtcg
     5941 tcggccgtgt aggcgcggtg gggatcggtg gccgcgagcg ccatcgcgcc gacgagccgc
     6001 cgccccgctc gcagcggcac cacgatgagc gacgtcacgc cgtgctcgtg catctgccgc
     6061 gtgtgctccg ccgagtgcgc gaactccgcg agccgcgccc cgctgacctg cgggtagagc
     6121 acggagtcgc cggtgtcgag caggcgccgg agcccctgcg ggttgcgctc ggccgcctcc
     6181 tcggccgacg ccggcggccg cccgtgcgcc ggccgcacgc ccgcgcgcga gtcgaccacc
     6241 gtgaggatgc ggccgctcgg atcggggatg tcgatccagc accagtccgc gaggtcgggc
     6301 accggcagcc gcacggcctc cgcgagggtg ttgtggtagt cgctggcggt gtcgagcagc
     6361 tccgccgtgc gcgcgcggag ctgctgcgcg atctccgagc ggcgccgctc ggagatgtcg
     6421 cggatgaagc cggcgaaggt ggcggcgccc gcgccgggca ttcgcgtgat ggtgagcgag
     6481 accgggaacc gcgtgccgtc gccgcgcagg gcgtcgagct ccaacggctt cccgacaacc
     6541 cgcgactcgc cggtcgcgcg cacgcgcgcg agaccgtcgc ggtgggcggc ccgcagctcg
     6601 ggcgggatca ccagctcggc caggtcgcac ccgagggcgt cggcggccgc gatgccgaag
     6661 atcgcctcgg cggcggggtt gaagagcgtc acgacgcccg tctcgtccat cccgataacc
     6721 gccgtcgggg tcaccgagac gatgccgccg accgccgcgg cggcctcgag gtcgcgcgcg
     6781 gccagatcgc tacggcagcg ccggaccgcc gcgaagtagg ccagcgcggc cccggccgcg
     6841 acgatcagca gccggacggc ataggcgtcg tcgaagccct cgtcgctcca gctcccggcc
     6901 accgcggcca ggacgaccgc aatcgatccc ccgatggcca cgacccgcgg gccgaccact
     6961 acggcgagca ggatcggcgt gagcacgaac gtgccgacga gcgggcccat cgcgacgtca
     7021 cccgcgacga gggccgcatc cacggccagc gccagagcca gccatcgcat ctcgcgccgg
     7081 tcaaccacgg aggggatgta cccgggccgg cggtgcgtac ccgtttgggt ggcgaggagg
     7141 tccggactac gcgaacgccg cggccggctc gggcccggcg acctcgttcc aggtcgcccg
     7201 cagcgcgaac gaggccgcca gccaggcgac gagggcgccg gccgcggcga gcaggaagag
     7261 cggacgcggc ccgagcacgg cggccgcgcc gccggccagc aggaacgccc cgccgaaccc
     7321 gaacgacacg agcgcgctcc tgagcccgaa gacccggccc atgatctcgt cggcgagcgt
     7381 ggactggagg agcagccgct cgtgcaccac cgccagcccg tttccgacgc cgagcgcggt
     7441 gaacgccggc agcgcgacgg cgaagctcgg cgcgagcccg cacgcgaaca ggccgcccgc
     7501 gcacatgagg acgccccaca ggtagcggcg cttgagcacc ggcggcgcgc cgccctcggc
     7561 gcccagcagc gtcccgccgg ccacaccgag ccccatcgcg gtcaccagga tcgagaactg
     7621 cgtgtcggag gcgcccagct cctcgcgggc gagcagcagc tcgccgacgt tgaccatccc
     7681 aaggaacgcg acgcacgaga ccgacgagaa gagcagcgtg agggtcccgc gcgcgcgccg
     7741 ggccgccacc atgccgtcgc gcaccgaggc gagcatctgg ctgcgctcct tggcccgcgc
     7801 ctcctcgctc gcgaacgcca gcgtcatgag caccgcggcc gaccccagga aggtgatcgc
     7861 gttgaccagc agcatcgcct cgggcgcgag cacgacgaac gcggcgccgg ccagcgcggg
     7921 cccgaccatg tagccgagct cctcgatcgt cccgtagagc gaggtcgcgg ccgggaagcg
     7981 ctcgcgcgag acgaggcccg gcagcgccgc catcaccgtc gggttgaaca tcgcgttgcc
     8041 tgtgcccgcg agcagcgcga gcgcgaaggt ggcgaagaag ccgtcgacca gcaccagcgc
     8101 gaggaacgcc gtggcccgca gcacgtcggc ggcgatcagc aggcgcttgc gcgacccgcg
     8161 gtcggccgcc gcgccgaaga tcgggccgag cagcatcgcc gggatgaact ccgccagcag
     8221 gatgagcgtg atggcccacg gcgagcggaa gcgctcgtag gcgaggagga gaagggcgac
     8281 gtagccggcg ccggtgccga gcgacgactg cgcgtgcacg aggaagaagc ggcgggcgct
     8341 gcgctccgcc ttcagcagct cgaggattgg cttcatagtc ggtcgaccac agcgcgggag
     8401 acacggcggc aattgccctc cgcctcccgg atgccggtga cggaggggcg atcgccgacg
     8461 aactagctcc agctggcgcg catgcgagtc cctttcgttg aggcaggtcg gagcaagggc
     8521 cctcgcggcc agatgcgaag catccccgag caccggcccc ctgccgattt acccgatgag
     8581 gcgaccctat gtgcggaccc ccgggcccgg ccatagggac attcgtcctg cgtgggcaga
     8641 cggccctccg ccaccggagg gccccggtgg cggaggacgg tcgccgaacg gcctaggtcc
     8701 agctggcacg catgcgagtc cctttcgatc gcttacctcg gctcgcccga gcgtaagttc
     8761 caaggccgcg accggccata gggacattcg tcctccccgc cccgagggac gcgggtatat
     8821 aaccccccat ctgagggagc gcgccgggac cggcaggggt gccgcgccgc cgccaggagg
     8881 tgaggcgggc gcgatggcgc gcgcgctcgc ggccctgccg gagtacccgg tccacgcgca
     8941 ggcccgggag cggctgcggg tggcgatcac cgagcggtcg gcgggagcgc agctcgcggc
     9001 gatcgtccgc tccgacgccg ggctcgccgt cgccgcactg cgcgccgccg ccggcgagct
     9061 gggcgacgag tgcggcgccg agcgcgcggt cgaggtcctc ggtgccgagc ggctcggcg
//
LOCUS       DASVPL010000281         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_387351, whole genome shotgun sequence.
ACCESSION   DASVPL010000281 DASVPL010000000
VERSION     DASVPL010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387351"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VF517_15625"
     CDS             <1..895
                     /locus_tag="VF517_15625"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8054416.1"
                     /translation="NASTPVRVVLESGAGPAADRYGAALRGLEGVVDVRRAALGAART
                     QYDLVPADAPFAEPTRELVRRVRAVPAPAPVAVGGDTAAFLDQSSSVRRHVVPAALVA
                     GAVTLLVLFLMTGSVVLPVKTLLMNALTACATFGVLTLVFQHGWLSGLLGFESRGAFV
                     DPTLLVYVAALAFGLSTDYAVMMLARIREAHGGGARSDRAVALGLERTGRIVTAAALL
                     FAIAVGGAATSELVAVKEAAVGTVVAVILDATIVRSLLVPSLMALLGEWNWWAPAPLR
                     RLHERLASRLGESPRGSLSSV"
     gene            901..1725
                     /locus_tag="VF517_15630"
     CDS             901..1725
                     /locus_tag="VF517_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8054417.1"
                     /translation="MRLYATGDLHLTHRQNVDVAASFPYRPDDWLALVGDVGVTAEHL
                     REAFELVRPRFARVLWVPGNHELWTHPSDPDQDRGEERYLRLVELCRSYDVLTPEDPY
                     AECDLGGERLVVAPLFTLYDYSFRQPGETKEEALDEARRAGAFCADERLLHPDPHASR
                     EEWCHARVRATEARLAALPSDRRTVLIAHFPLRQDVVRLPRIPRFSPWCGTKLTEDWH
                     VRFRAAAVVGGHLHVPGTLWRDGVPFSEVSLGYPQQWSNRPWPHGAPREVQLGAAN"
     gene            1745..2722
                     /locus_tag="VF517_15635"
     CDS             1745..2722
                     /locus_tag="VF517_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054418.1"
                     /translation="MPSPEAVDEVQIAPAGALVSFDRWERQTWTVAELDLAHDRAGWS
                     EMPSFLAAELRFNVDRFFLGEAAVTETLAPLAHSAPEPEWQLYLCTQLSDEARHTMFF
                     VRYLEAVGVREPEGELRDYLQRRWDETPAHFTELLDRQLREVTTRAWSSDDEADWFRA
                     VTLYHLVVEGVLAVTGQRRLLDIIGDRPGLATLQAGILNIARDESRHIGFGVGALREG
                     VRRGFGDAITAQLEQSVPPAARIALSPERPLPGLLPARVQRSIGTDFERRLGKAGEAL
                     AGRVERIGLAGAAAGVRRMWEGALGEALDDYRSSHGREHPLRAAQAVRS"
BASE COUNT          329 a         1043 c         1066 g          389 t
ORIGIN      
        1 caacgccagc accccggtgc gcgtggtcct cgagtcgggc gccggcccgg cggcggaccg
       61 ctacggcgcc gccctgcggg gtctcgaggg cgtggtggac gtgcggcgcg cggcgctcgg
      121 ggcggcgcga acgcagtacg acctcgtccc ggcggacgcg ccgttcgcgg agccgacgcg
      181 tgagctcgtg cgccgcgtgc gagccgtgcc cgccccggcg cccgtggcgg tgggcggcga
      241 tacggcggcc ttcctcgacc agagcagcag cgtgcgccgc cacgtggtcc cggctgcgct
      301 cgtggcgggg gccgtcacgc tgctcgtgct gttcctgatg acgggatcgg tggtcctccc
      361 ggtcaagacc ctgctgatga acgcgctgac cgcgtgcgcg accttcggcg tgctcacgct
      421 ggtgttccag cacggctggc tgtcggggct gctcggcttc gagagccgcg gcgcgttcgt
      481 ggacccgacc ctgctcgtct acgtcgccgc gctcgcgttc ggcctctcca ccgactacgc
      541 ggtgatgatg ctcgcccgga tacgcgaggc gcacggcggc ggggcgcgca gcgaccgggc
      601 ggtggcgctc gggctcgagc ggacgggccg catcgtcacg gccgcggcgc tgctgttcgc
      661 gatcgcggtc ggtggtgccg ccaccagcga gctggtcgcg gtgaaggagg cggccgtggg
      721 gacggtggtc gcggtgatcc tcgacgcgac gatcgtccgc tccctgctcg tgccgtcgct
      781 catggccctg ctcggcgagt ggaactggtg ggcgccggcg ccgctgcggc gcctccacga
      841 gcggctggcc tcgcggctcg gcgagagccc gcgcggctcg ctctctagcg tctgagccgg
      901 atgcggctct acgcgaccgg cgacctgcac ctgacgcaca ggcagaacgt ggacgtggcg
      961 gcctcgttcc cgtaccgccc ggacgactgg ctcgcgctcg tgggcgacgt gggcgtgacc
     1021 gccgagcacc tgcgcgaggc gttcgagctc gttcggccgc gcttcgcccg ggtgctgtgg
     1081 gtgcccggca accacgagct gtggacacac ccgagcgacc ccgatcagga tcgcggcgag
     1141 gagcgctacc tgcgcctcgt cgagctctgc cgctcctacg acgtgctgac gcccgaggac
     1201 ccctacgccg agtgcgacct cggcggcgag cggctggtcg tggcgccgct gttcacgctc
     1261 tacgactaca gctttcgcca gcccggcgag accaaggaag aggcgctgga cgaggcacgc
     1321 cgcgcgggcg cgttctgcgc cgacgagcgc ctgctccacc ccgacccgca cgcgagccgc
     1381 gaggagtggt gccacgcgcg cgtgcgcgcg acggaggccc gcctcgccgc gctgccctcg
     1441 gatcggcgca cggtgctgat cgcgcacttc ccgctgcgcc aggacgtggt ccggctgccg
     1501 cgcatcccgc gcttctcgcc gtggtgcggg accaagctga ccgaggactg gcacgtgcgc
     1561 ttccgcgccg cggcggtggt cggcggccac ctgcacgtgc ccggcacgct gtggcgggac
     1621 ggggtgccgt tcagcgaggt ctcgctgggc tatccgcagc agtggagcaa ccgcccgtgg
     1681 ccgcacggag cgccgcgcga ggtgcagctc ggagcggcga attagccctg gtactgtggc
     1741 gcaggtgccc agcccggagg ccgtcgacga ggtgcagatc gccccggccg gagcgctcgt
     1801 ctcgttcgac cgctgggagc gccagacctg gaccgtcgcg gagctcgacc tcgcccacga
     1861 ccgcgccggc tggagcgaga tgccgagctt cctggcagcc gagctgcgct tcaacgtcga
     1921 ccgcttcttc ctcggcgagg ccgctgtcac cgagacgctc gcgccgctcg cgcactcggc
     1981 gcccgagccc gagtggcagc tctacctctg cacccagctc tcggacgagg cgcggcacac
     2041 gatgttcttc gtgcgctacc tcgaggcggt cggcgtgcgc gagcccgagg gcgagctgcg
     2101 cgactacctc cagcggcgct gggacgagac gcccgcgcac ttcaccgagc tgctcgaccg
     2161 ccagctccgc gaggtgacca cgcgcgcatg gagctcggac gacgaggccg actggttccg
     2221 cgcggtcacg ctctaccacc tggtcgtgga gggggtgctt gccgtgacgg gccagcgccg
     2281 cctgctcgac atcatcggcg accggccggg cctcgccacg ctccaggccg ggatcctcaa
     2341 catcgctcgc gacgagtcgc gccacatcgg cttcggcgtc ggtgcgctgc gcgagggcgt
     2401 ccggcgcggg ttcggcgacg cgatcaccgc gcagctcgag cagtcggtcc cgcccgcggc
     2461 gcgcatcgcg ctctcgcccg agcgcccgct gccgggtctg ctgcccgcgc gcgtgcagcg
     2521 ctcgatcgga accgacttcg agcgccggct cgggaaggcc ggcgaggcgc tcgcggggcg
     2581 cgtcgagcgg atcggactgg ccggtgcggc ggccggggtc cggcgaatgt gggagggcgc
     2641 gctcggcgag gcgctcgacg actaccgctc atcgcacggc cgcgagcacc cgctgcgggc
     2701 cgcccaggcg gtgcgctcgt gagcggccgg gacgtccacg agcgcgtcgt gcgaacggtg
     2761 ctgacctgcg ccccgcgccc ggccgaggag gtcgacgcga gcacggacct cgagaacgac
     2821 ctcggct
//
LOCUS       DASVPL010000282         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_189783, whole genome shotgun sequence.
ACCESSION   DASVPL010000282 DASVPL010000000
VERSION     DASVPL010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189783"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..990)
                     /locus_tag="VF517_15640"
     CDS             complement(34..990)
                     /locus_tag="VF517_15640"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX8054419.1"
                     /translation="MADLQAKDLKLIQYLNEAYGKEKQLETALQAHISMTTRPPYKKR
                     LQQHLKETKGHAREVERRIKKLGGTAETVDVPGPDAVSGAAQAVQNVANRAAALAQGP
                     MHALRGTGEQERLLKNARTEFQDEAEEIATYTMIETLAEAVGDKETAQVARKIRREEE
                     RMRDFLAKLIPTLTKAVAQEEIPAAERRTSSRRSSSRRSSSSRSSGGSSSSSSRSSGS
                     SSRSRSGSSSGGTSRSRSGSSASKSSGSRSSGSSRSSSARKSSGSSRSSSSASKSSGS
                     RSSGSSRAKSSGSGSSRAKSSGSSRAKSSGSRSGGSRSGGSK"
     gene            complement(1121..1945)
                     /locus_tag="VF517_15645"
     CDS             complement(1121..1945)
                     /locus_tag="VF517_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX8054420.1"
                     /translation="MRTAVVGHVEWCEFARVERTPAPGEIVTASSTFVEAAGGGAVAA
                     VQLARLAGDCTLYTALGDDDVGTRAHARLTELGVRIEAAIRPSEPTRRAFTHVDRAGE
                     RTITVMGERLEPRRADDLPWDELAGVDALYFTAGDAASLRAARAARSLVATARVMDVL
                     DAASTRLDAVVASAADPSERYRPIDPPPALVAKTAGGSGGTWSAHEGRTGAWAAAAVP
                     GPIRDAYGCGDSFAAGLTFALGAGHAPDAALAIAAECGAACLAGDGPYGYALARRA"
     gene            complement(1949..2305)
                     /locus_tag="VF517_15650"
     CDS             complement(1949..2305)
                     /locus_tag="VF517_15650"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8054421.1"
                     /translation="MLQHVTLEVPSELVGACAEFWSLLGFERVKPPASLGDRAVWMER
                     EGTQIHLMHVDDPVVPRQGHAAIVVADYGRVFAALTAAGYDPQPRTEHWGAPRCFVRD
                     PAGHRVEVMAASPARG"
     gene            2327..3109
                     /locus_tag="VF517_15655"
     CDS             2327..3109
                     /locus_tag="VF517_15655"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX8054422.1"
                     /translation="MLGRAKRAARRALLARGVEVSRRGGARRTMAAVLDNMKRIGLDP
                     GTVIDVGVGYGTPELWEAFPRARLLLIEPIPDRFGNAAGRAASARAGAELVTAAAGAD
                     AGNATITVHRAPEMSSLLGTWIGDRDGGERREVPVVRVDDAVRDRGLPGPYVLKVDVE
                     GAELDVLDGAAAVLGETEVVLLEVRLFELVPGGAQLHDVVPYMKERGFVAYDLFGGAI
                     RLTDGALAVTNLAFVREDGMFRRDHAFGTPEQVDRMARELGN"
     gene            complement(3228..3680)
                     /locus_tag="VF517_15660"
     CDS             complement(3228..3680)
                     /locus_tag="VF517_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054423.1"
                     /translation="MNGRRLPEAVYWRRRAVAGGLILFFVIVVVAIASGGDDDGGTPE
                     TLPPPAAAPPVEDTAPAEDTDEEDTDDTDAAPSGGGSAPAPSGGGSTPPAGGGGGATP
                     PAGGGGGGSAGGGGGSEGGGGGTPPGRDEGTGGAAPPGSLPSNGGRSP"
BASE COUNT          438 a         1385 c         1493 g          498 t
ORIGIN      
        1 gcccgcgcga ggcgggcgcc gccggcctac gaactacttg ctgccgccgg agcgcgagcc
       61 gccgctgcgc gacccgctcg acttggcccg cgaggagccg ctcgacttgg cccgcgagct
      121 gccgctgccg ctcgacttcg cgcgcgagct gccgctgctg cgcgacccgc tcgacttcga
      181 cgctgacgag ctgctgcgcg acgacccgct cgacttcctc gccgagctcg agcgcgagga
      241 gccgctgctg cgcgacccgc tcgacttcga cgccgaggag ccgctgcgcg accggctggt
      301 gccgccggac gacgagccgc tgcgtgagcg gctggagctg cccgaggacc gcgacgagct
      361 cgacgagctg ccgccgctcg aacgcgagct cgagctccgc cgtgacgagc tgcgccgcga
      421 cgacgtgcgc cgctcggccg ccgggatctc ctcctgcgcc acggccttcg tgagcgtcgg
      481 gatcagcttg gcgaggaagt cgcgcatgcg ctcctcctcg cgccggatct tgcgcgcgac
      541 ctgcgccgtc tccttgtcgc cgacggcctc ggcgagggtc tcgatcatcg tgtaggtcgc
      601 gatctcctcg gcctcgtcct ggaactcggt acgagcgttc ttgagcagtc gctcctgctc
      661 gccggtgccg cgcagcgcgt gcatcgggcc ctgcgcgagc gcggccgcgc ggttcgccac
      721 gttctgcacg gcctgggccg ctccgctcac cgcgtcgggg ccgggcacgt ccacggtctc
      781 cgccgtgccg ccgagcttct tgatccgacg ctcgacctca cgagcgtgtc ccttcgtctc
      841 cttcaggtgc tgctggaggc gcttcttgta gggcgggcgc gtggtcatcg agatgtgcgc
      901 ctggagcgcg gtctcgagct gcttctcctt gccgtacgcc tcgttgaggt actggatgag
      961 cttcaggtcc ttggcttgta ggtcggccac gttggacgac actcccttcc attgggtcaa
     1021 gttcgtaatg agcgtgcggg gggttttccc tccggcgacc ttacccgcat ctactgcaag
     1081 acattcacgc ggcggcgcgc gaggacgcgc gcacgacgga ctacgcgcgg cgcgcgagcg
     1141 cgtaaccgta cggtccgtcg cccgcgagac acgccgcgcc gcactcggcc gcgatggcga
     1201 gcgcggcgtc cggagcgtgg cccgcgccga gcgcaaacgt gaggcccgcg gcgaagctgt
     1261 cgccgcatcc gtacgcgtcg cggatcgggc ccgggacggc ggcggcggcc cacgcgccgg
     1321 tgcgtccctc gtgcgcgctc cacgtgccgc cggacccgcc ggcggtcttc gccacgagcg
     1381 cgggcggcgg gtcgatcggg cggtagcgct cgctcgggtc ggcggcgctc gccacgacgg
     1441 cgtcgagccg cgtggaggcc gcgtcgagca cgtccatcac gcgcgcggtc gcgacgagcg
     1501 agcgcgcggc gcgcgcggcc cggagcgagg cggcgtcgcc cgcggtgaag tagagggcgt
     1561 cgacgccggc gagctcgtcc cacggaaggt cgtccgcgcg gcggggctcg aggcgctccc
     1621 ccatcaccgt gatggtgcgc tcgcccgcgc ggtcgacgtg ggtgaaggcg cgacgcgtgg
     1681 gctcggacgg gcggatcgcg gcctcgatgc gcacgccgag ctcggtcagg cgagcgtggg
     1741 cacgggtgcc gacgtcgtcg tcgccgagcg cggtgtagag cgtgcagtcg ccggcgagcc
     1801 gggcgagctg caccgcggcc accgctcccc cgccggcggc ctccacgaac gtgctcgacg
     1861 cggtcacgat ctccccgggt gccggagtcc tctccacgcg cgcgaactcg caccactcga
     1921 cgtgcccgac caccgcggtc cgcacgagct acccgcgcgc cggcgacgcg gccatgacct
     1981 cgacgcggtg cccggccggg tcgcgcacga agcagcgcgg ggcgccccag tgctcggtgc
     2041 gcggctgtgg gtcgtagccc gcggcggtca gcgccgcgaa gacccgcccg tagtcggcga
     2101 ccacgatcgc ggcgtggccc tgccgcggca cgacgggatc gtccacgtgc atgaggtgga
     2161 tctgcgtgcc ttcgcgctcc atccacacgg cgcggtcgcc gagcgacgcg ggcggcttca
     2221 cgcgctcgaa cccgagcagg gaccagaact cggcgcacgc gccgaccagc tcactcggca
     2281 cctcgagggt cacgtgctgg agcacgcgcg cattatcgtc agggacgtgc tcggacgggc
     2341 taagcgcgcc gcgaggaggg ctctgctcgc gcgcggcgtg gaggtgagcc ggcgcggcgg
     2401 cgcacgccgg acgatggccg cggtgctcga caacatgaag cgcatcgggc tcgacccggg
     2461 caccgtgatc gacgtgggcg tgggctacgg cacgcccgag ctgtgggagg cgttcccgcg
     2521 tgcgcggctg ctgctgatcg agccgatacc ggatcgcttc gggaacgcgg cggggcgggc
     2581 ggcgtcggcg cgggccggtg ccgagctggt cacggcggcc gccggggccg atgccgggaa
     2641 cgcgacgatc acggtgcacc gcgcgccgga gatgtcgtcg ctgctcggca cgtggatcgg
     2701 cgaccgcgac ggcggcgagc ggcgcgaggt gccggtcgtg cgcgtggacg acgcggtgcg
     2761 cgatcgcggc ctgcccgggc cgtacgtgct gaaggtcgac gtcgagggag cggagctgga
     2821 cgtcctggac ggcgccgcgg cggtgctcgg cgagaccgag gtcgtgctgc tcgaggtgag
     2881 gctgttcgag ctggtgccgg ggggcgcgca gctccacgac gtggtcccgt acatgaagga
     2941 gcgtgggttc gtggcctacg acctgttcgg cggggcgatc cggctgaccg acggggcgct
     3001 ggcggtcacg aacctcgcgt tcgtgcgcga ggacggcatg ttcaggcgcg accacgcgtt
     3061 cggcacgccg gagcaggtcg accgcatggc gcgcgagctg gggaactagg gcggcggccg
     3121 cccgcgggcg gcgacgccgg agccaggctg tggccgccgg cgcccagtgg acggcgacgc
     3181 cggagccagg ctgcgaccgc tggcgtccag tgacggcgac gccggagcta ggggctgcgg
     3241 ccgccgttgg agggcagcga gccgggcggg gccgcgccgc cggtgccctc gtcgcggccg
     3301 ggcggcgtgc cgccaccgcc gccttcggag ccgcctccgc cgccggccga accgccaccg
     3361 ccgccgccgg ccggaggggt cgcgccgcca ccgccgccgg cgggaggcgt ggagccgccg
     3421 ccgctcggag cgggcgccga gccgccgccc gacggcgcgg catcggtgtc gtcggtgtcc
     3481 tcctcgtcgg tgtcctccgc gggcgcggtg tcctcgaccg gcggcgcggc cgcgggaggg
     3541 ggaagcgtct ccggcgtgcc gccgtcgtcg tcgccgcctg atgcgatcgc gaccacgacg
     3601 atcacgaaaa acaggatgag gccccccgcg acggcgcgcc ggcgccagta gacggcctcg
     3661 ggcaagcgcc tgccgttcac ggcccgaacg taccacgcgc ggaggcgcgc cacgcgggta
     3721 ggggcggggc cggcggcgcg gcttcggctc agccgggcgg ctgctgcggc tgctggggct
     3781 gctggggctg ctggcccgga ggcgtgcccg gcgg
//
LOCUS       DASVPL010000283        23276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_307904, whole genome shotgun sequence.
ACCESSION   DASVPL010000283 DASVPL010000000
VERSION     DASVPL010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 23276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23276
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307904"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF517_15665"
     CDS             complement(<1..225)
                     /locus_tag="VF517_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054424.1"
                     /translation="MTGDDFNRLLNGSRLLVGVAALAAPGKAFSGVGLDSRRNPQLPF
                     VTRMFGVRDLVLGAGALGTSGGERRRWLQAA"
     gene            complement(222..1295)
                     /locus_tag="VF517_15670"
     CDS             complement(222..1295)
                     /locus_tag="VF517_15670"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEX8054425.1"
                     /translation="MGPLKGLILSGGRGTRLRPITFTRAKQLVPVANKPVLFYGIESL
                     AAAGIEEIGIIIAPETGGEIEAAAGDGSRFGVKITYIEQDAPNGLAHAVLTAADFLGD
                     SPFVMYLGDNLLRDGIVELVQQFRSQEPDALILLTPVPDPQNYGVAELDGERVARLVE
                     KPEQPQSDLALVGVYMFTPAIFEAARAIEPSWRDELEITDAIQRMIDSGLTVDPHIVR
                     GWWKDTGQVQDMLDANRLILDDLGERIEGELIESRVEGRVVIETGAVLERSTVRGPAV
                     IGRGARITDAYIGPYTAIGEDVTIHGSEIEYSIVLAGSSVRDLHGRVEASLIGRNVAI
                     GRSPALPKAYRFVVGDNAEIAIL"
     gene            complement(1296..1739)
                     /locus_tag="VF517_15675"
     CDS             complement(1296..1739)
                     /locus_tag="VF517_15675"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX8054426.1"
                     /translation="MSDEELRDFLAEQMIVSVATVGPSGRPHLVPLWYVPEDGPTLTG
                     WTYAKSQKAKNLERDPRATLQVEDGIQYHELRGVMLECDVQLEHDTEKVTGYGMALFE
                     RYAGGTELPPETRDMVAGQAAKRVGLRFVPTRVVSWDHTKLGGTY"
     gene            1830..2294
                     /locus_tag="VF517_15680"
     CDS             1830..2294
                     /locus_tag="VF517_15680"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX8054427.1"
                     /translation="MRSEREARMQNLGGRVPWFGSAVPRLHARLYRRLGGRFVGRWFG
                     GPVAVLETVGRRSGRPRAVPVIYAWDGEDVVVVAANAGNPRPPAWWLNLREQPRATLV
                     IGGERRGVRAREARGEERERRLAIYKRVYAKVDDYGAFTDREFPVVVLEPEP"
     gene            2381..3364
                     /locus_tag="VF517_15685"
     CDS             2381..3364
                     /locus_tag="VF517_15685"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8054428.1"
                     /translation="MAVAERLLAIQGQDARGFRLAVRARTRGLTAADVERCLGEERSL
                     LVTWVNRGTLHLIRSEDYPWLHALTTPPLLTGCMRRLAQEGVEPGAADRAVDVIERAL
                     IEEGPLAREQLRDRLRSAGLRTEGQALIHLVFLASLRGVCVRGPMIGKQHAYVLVRDW
                     LGEAPPVDRDRALAELARRYLAGHGPAGDRDLAKWAGLPLRDARAGLEAIAGEVVERE
                     DGLVELARGGSGRGGGAAPPPAELPPPVLLGAFDPCLLGWATREPIVGEHDSAIVSGG
                     IFRPFALVKGRVAGVWRLERGAVKLEPLERIARADAEALDADAADVRRFLK"
     gene            3481..5229
                     /locus_tag="VF517_15690"
     CDS             3481..5229
                     /locus_tag="VF517_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8054429.1"
                     /translation="MHRLTRVSLAGFQSYQVRQEISLDPDLTFLVGRNNVGKSAFLRA
                     LRAFADPTEYVQPGLDVTYTWHVTSDVIVNQLDPKLGAAVKEIGSYPAHELVASYVAA
                     QTPGTQQAAMYCYRLELPELGWHAEGQVGYKAGWNSGPWKQSSTGFQELEQLVRRWVT
                     AVELIAPRRVDQGRRGLYPQSRLAPEARNLTEVLFDLQLNKPSGTFRDLIAFMKEAFP
                     EIDTITVRTPSDESSPMGEIHIIYGNTSQSVPLRLCGTGVEQMLALGTAVLNAGSPRL
                     VLIDEPQAYLHPHAERSLLSLVEAHPEHQYVVATHSGLLLNARPISHSRLITIENGSS
                     RISDPDSDSGILTELGIGPADLWLPDRLLWVEGASDETVFRTLLKEEVDPASAAGLRI
                     RPMPESSRFTGRTRRHADAAYRFCKEVADAVSPLSVEMLFLFDRDEKDSSIMDAISEA
                     SSNRARFLPVREIENLFLDVDVLHAAVTHRAETLEQAPPSRSEVQTFLAQLLAAAGDR
                     RLFPSPPRQPESAVVDVRGSAVLDEMFWRYTTSRYDKIRDAKLLTELALQHAPHVLDP
                     LRAVLTEFALQRQTVN"
     gene            complement(5226..7286)
                     /locus_tag="VF517_15695"
     CDS             complement(5226..7286)
                     /locus_tag="VF517_15695"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8054430.1"
                     /translation="MAKDTEKLIRQLSLISFLMAERRPVTALEIKQEVEGYSGMNDDA
                     FARRFYADRAELESLGIELKVDKPAEGYYEAENYSLPPQNFYLPSIKFTDEELGALRT
                     ALSLLDGEFAYAEPLRLALQQLSWGKPSPLSAPENRQVALGVTAGAGGRELSQRLAKV
                     ETAIFRRKTIVFDYYTMGRDTQEQRRVDPYHLLFRGGHFYLVGHSHERDDVRVFRLSR
                     IQGKVGYSSKAEHDFPPPEDFDPRGYATRTDWQLGDTIGTARIWVAPQIDWLVERHFG
                     EAGTIVPAAEVDDAPEDGKVFETAYGDERQIVSWLLGLGERARILGPPELAERASAAV
                     RTIVERHSTPLEPAPAARRKPVVAAPEADSGRGEALIRPERFARLVTLAGILIDAART
                     GSKLDTRQVRESLQLTDIELREDIDVLNVVNFGGGSYVLYAEVHDDTIEVDPEPYGDN
                     FAKPARLLPLEAKALVAAIDLIGDHLPQGSLASARGKIVEALGRDPATDGLQITTAAG
                     DDSEIARIVSQAIADHRLLRLDYYKENEDEFTTRRVEPYQLLNGQEGWYVHCWDLDRD
                     ATRSFRLDRIREAEILDEEFEPRPGIEPDVQGWPRTGEVEASRAARVWISPERARWAR
                     EDRRVVEELRDGAVIVEVHYAGRDWLVRQILEEAGDAVVFEPEDAREAVLQAAEALAG
                     AAAR"
     gene            complement(7505..8587)
                     /locus_tag="VF517_15700"
     CDS             complement(7505..8587)
                     /locus_tag="VF517_15700"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8054431.1"
                     /translation="MDPGPAQPQLDGSPAFIEEAPLAGGLPPAAAALPAKVFVRDLAD
                     GARVVSPFVVREVTRRTKRNGEAFLKLKLGDVTGTVEGVVWDGVESACEVAVLGAVVI
                     ANGAYECDAKYGACLTIRSLRPARAGEYEPADLHDGPVESCDRMVAALRELIGTIQDP
                     HLRLLLDGFFAEDCAVWERWRDAPAAKHYHQAYRHGLMEHCLSVAQGVSAMSSTFPGL
                     DRDVAVTGALLHDIGKIEAYAMEGGAIDLTDAGRLQGEIPLGYYRVRRAIEDIPDFPP
                     DRAEAVLHIILSHHGQLEHGSPVVPCTREATLVHMIDNLGGRLGSFDRIEKGLQNGER
                     WSSFDRALSGPAYFGARGIAGLGEAA"
     gene            8649..9356
                     /locus_tag="VF517_15705"
     CDS             8649..9356
                     /locus_tag="VF517_15705"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8054432.1"
                     /translation="MPDAPAAASAVLVAGGTGALGTAVVRELLDAGRPVTATWLVERE
                     RERVEAELGGDDLLTLVQADLMELEGAEAAVAATDGLGAVVNLVGGFASGPLAHETHP
                     DEFDRMLRLNVRPGCTLVCAAMPRMLEAGGGAYVGVSARPALRPFAGAASYVTAKAAV
                     LAFVQALDADYRKHGIRANAILPSVIDTPANRAAQPDADHSDWVQPAEIARVVSFLVS
                     GDAAVTSGGAIPVYGRA"
     gene            9373..9879
                     /locus_tag="VF517_15710"
     CDS             9373..9879
                     /locus_tag="VF517_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8054433.1"
                     /translation="MTTIPSQGILSEDKQAERDELIALLTKAYWMELETVMSYISNST
                     NPDGVRAQEIIESLKQDVQEELGHAQEFANRIKELYGVVPGSLDFQAEQSYLQPPEQQ
                     TDIVHVIKGVIQAESGAIEHYNRIIEFCEGKDPVTQDMVIAILRDEEGHRRLFEGFLR
                     EYQVEGRA"
     gene            complement(9920..10768)
                     /locus_tag="VF517_15715"
     CDS             complement(9920..10768)
                     /locus_tag="VF517_15715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX8054434.1"
                     /translation="MTTTVAGRTRIEGLRGRGAGGSFGDGSVAWTVHRERVLLLSGGR
                     SLLMQAAHPLALAGFLGHSAYDEEPWGRLWRTAHSMMMAIFGTREQAEAVGERVRAMH
                     TRVNGELTEPLGSFPAGTRYSAEDPELLLWIHANFVHGAIVVYEALVRRMSDRDKDAY
                     HEEMKAVAELFSLPAAAIPATWADLDDYVEDMVASDVVTVTQQAREVARGTVLGPPLP
                     LGLRQGWPLVNFLIAGLLPEPIRRGYGLGWTPAHTALFTATMAAGKRLAIPLVPTPLK
                     RYSPRD"
     gene            10852..11271
                     /locus_tag="VF517_15720"
     CDS             10852..11271
                     /locus_tag="VF517_15720"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8054435.1"
                     /translation="MGGMSSVVRRSSDQTLARVFARRPARRLLVATVVRFIPRRAPEN
                     LDGCVLVELVSPPTRRREKRSEPWVFEFSEGSARARRGEVSEPQLSVSVAVPDFVRIA
                     AGQLDPMSLVFDKRIRMKGSFALARRLFALAPGDEAQ"
     gene            11268..11987
                     /locus_tag="VF517_15725"
     CDS             11268..11987
                     /locus_tag="VF517_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8054436.1"
                     /translation="MTQTAAETEAPVVVETVGLVRVYGEGDNAVQALRGVDLQVRRGE
                     FVAVVGPSGSGKSTLLHLLGGLDRPDAGEVLLDGTSLASLPDKELGLIRRRQLGFVLQ
                     FFNLLPTLDAAENAAFPLSLDGVPDAIDRARTALGLVGLAGREDHRPSQLSGGEQQRV
                     AIARALVTRPSLLLADEPTGNLDSRTGQEIVDLLRRTADEGQTIVMVTHDLRWTGVAD
                     RVVRIEDGQIATSDETASVAP"
     gene            11984..14569
                     /locus_tag="VF517_15730"
     CDS             11984..14569
                     /locus_tag="VF517_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8054437.1"
                     /translation="MRAFAGLFRRINARHFGEQRLRTLITIGGISAGVALTVSIAVIN
                     ATLQVSVKATARDPTGAADVEVQPANLSGLPQSAVSSVASVPGVRDAVPFTRVAARVK
                     GDSGSVRTIVLGGDPGPTARLASRIEQADSLKITQRGSLSGGVLLTPDLADEIGAGVG
                     GRVEVETPHGPVELPVAGTVEGSAVERFNAGRVAFMHIATAQATFDRRGTVDAIYVVA
                     DPGTPIPQLQDAIRERLHGAGIVGDPGEVSRGFENTLGPLAAAGTISGLVALLVALFI
                     VYNTMSMAFAERRWEVSMALALGASRRQLAGAFLLEALVIGIIASAVGVLTGTASAYV
                     MAERAIEDYRGITVGLATPPVIHARDLLLGGLGGVIVTLLGAAIPARRVFGLAPIESL
                     RPEAAHEWAATVRGSSWTRRQIVAGLLAVALGTVGLLLSIRLVQEDWLATVTTALWLA
                     GVALLLPHALPIVGRALQPALARTFGALGRLAGEALTKNPGRTALTTGALIVSSSMAI
                     AVASGLDSYEQRFANTAEGWFGGDLYVVAGKSDNSVGADQPVDPSAADALRRVEGVRA
                     LYPQRYAVTRVGGEPALMYAIPIAQAAREGTTRTLSESGADQDALIRGLGRGEVGVSR
                     FTAARLDLKAGDTIPIATPTGTRRFRIAALFKDLAGFDTIQIEHSTYARYWNDRKVDR
                     FAVLAQPGANLGKLRADLGRAVEAAGLDGRVLEQQQAVEELVGGVEGLVSLARSIQLV
                     ALVVAALAIANTLFAAVIERRWEFGLQRVLGMSRSEVARTLLLEAGVIGLMGSVIGVV
                     LGVAMGYVMVRAMTAGYSWDIPFAMPWELIAFGLVGGVAIAAAAGVTPARIATRTSIV
                     AALRR"
     gene            complement(14570..15370)
                     /locus_tag="VF517_15735"
     CDS             complement(14570..15370)
                     /locus_tag="VF517_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054438.1"
                     /translation="MTVRSRLVPALLIALAVAGCGGDDGSGDAPVRNVDRPDPPTRPS
                     AEECAAVGRVAGGGESRAELVAPPGEYRFRTEGTQTLEGRSQPLPHETRLIVTPARAA
                     GALRCYRVQRLYSPRFGDTALAAVAGSSAYASEFVSQGGGQRAEFRLSPPIPLDTNEP
                     SGSGTFNGTLEAQRGSVSFEGPASGRYRSDFAGRRTITVAGRDVRAVGVRLTLAMAGG
                     EVKATEESERWVSDDNVLVEETVTQTRAIAGERFRMRYHAELKSLDPG"
     gene            complement(15367..17562)
                     /locus_tag="VF517_15740"
     CDS             complement(15367..17562)
                     /locus_tag="VF517_15740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEX8054439.1"
                     /translation="MSGARAVVDEAEFAVVGAGPAGSACAARLAELGHDVLLIDQGSF
                     PREKPCGDGLTPSAVAFLRRYGLDPVIDQSYPIAGLRGVLDYRHSRFRDFGARRGESA
                     CCVPRGRLDDALREAALARGARFMRARAQGPAIVDDGVEGVIVRGDDGEAQVAARYVV
                     AADGATSRMRREAGFTRPPNVPVAYGVRQYFRTERRLEPSFDVYVPVEHEGVPLLGYG
                     WVFPVSEHLANVGVGVWRVPGVDNPPPLRTVLSRFVEDLRLRAGRRVGDMEPTGEPLG
                     SPLATNFTRERCQLRNMLFVGDAASTTDPITGEGIAYALFGGELVANVGHDAARRGDR
                     APDPGLLMARRFPRLGQDISLVTRGGLRYGRELMKGEIELGGNTRLPFVKSIARMALE
                     AEEEPTLTGTPVRRTLADLDQSCADDLDELNNRTLDLLRTRFPFASELLHRELRAHAG
                     PLYGSVLLLANRACGGTPGEVLPAALAIECVRIVGRISTLVADPVRNQQARFNNAMAV
                     LISDFAVSRAALAASEHGQNVVSGLARAACSMCEGGMLDVEDRYDLERTPERYFAASA
                     ATAGAVVSTAARLGAELALAGDDQVAELADYGWHVGMASQVADDVVRLLAGDDQTAPR
                     PGTDLRWGIYTLPVVYGLQRDARLKRMLARGIEKEDLPEAVAAIRESGAIEAAVRDCE
                     QHVASATAIVERLALNGAGDPLVAVAELALERVRTAAGPAGEPSLAANA"
     gene            complement(17559..18524)
                     /locus_tag="VF517_15745"
     CDS             complement(17559..18524)
                     /locus_tag="VF517_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX8054440.1"
                     /translation="MGSYFEGAIATLRPDEIEGVAAGLPEGQTLTNACRHLLATKGKQ
                     LRSTIVLEAARYGPEPDSDVVRRVATATELLHYATLAHDDVVDDGQMRRKITTVAAEY
                     GHPAAAYAGGWLCARAVELVADTEFARDAAEAACAICDGEMLEVQDLGNVARTAESYF
                     DAIAGKTARLFSLASSLGAKASGAPEPVVAALTSFGHDLGMAFQISDDMLDVTGGEES
                     IGKPAGSDLRLGVFTLPVIYAMEEDPGLAPRLAEGVEDDDLAGVIDAIRATDGLERAQ
                     AHCDRHAEAAQAALASLESGDPGAGASLRALVDYTAARARPVGTA"
     gene            18738..19901
                     /locus_tag="VF517_15750"
     CDS             18738..19901
                     /locus_tag="VF517_15750"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX8054441.1"
                     /translation="MESSQSFAGGLKAARAHIGVITRDVWPPRTALLNLRRGLWPPSA
                     AVATFRRAREVWPPLLAIAKMSKVTFWFIWVTPLIFGYIASADSRGSAQDLIWFVLVL
                     AGVCFVESINCIHNELMDQEEDAANQPRRATLIASVGEGPLWLLVLVGYAVVAIGIVP
                     IVLFVGLDVALLITAASVAPVLYNAGPRLKRRAVMAELDIAWAVFFTYLTGWSFNQPI
                     GDVPAIIWLLTFFFGITSFLKDIPDIRGDQQVGAGGLFSIKRDNLRKVATAFVYVSPF
                     AVVAALVAVDELPDRMLWLLALLPLSVWLMVIGERVASLGTLIVAYESSFLYVHVFFL
                     VMFVLYTPTLAAAIAAAVLFAARVLVLYLGLAPRFVEPDFNWRTSFGALVRGA"
     gene            19904..21184
                     /locus_tag="VF517_15755"
     CDS             19904..21184
                     /locus_tag="VF517_15755"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8054442.1"
                     /translation="MAAERHYDIVVIGAGLAGLAGVIALRDAGLSVAVVDNIAKIDGS
                     RMNWGHDLQPNALAALESLGLLDRVRELGAWHDAYQLEKLGGGPLSHWDYSMLDHRHP
                     YAVCIRTHVLRELLREATRELDFVDLHIPATYTGLERTEGGHAVTIETGEGATERVHG
                     RLVVAADGPRSRVREAAGIRARFKRGKRHWLDVIMDNPGDRIKDGYVYFGRGEYLGIV
                     PTRPGELVAFHLTSTPNLDAYKQMIGGIDGFRARYAEMAPILAEPAQSVDSFDRTSHT
                     LGHSMRAETWVDDGLLLLGDSGVTVNPITSSGAGLALEEGVAFGEVVSECFAKGDLSA
                     AALAPYEERCRPRAEKMQDMGDTCTFVFGSRLPVVNGLKLRMLSRIDRDPAMKHRILA
                     YFSGMEPDPIGYRDGLAAAGLWPRGRRPAASMPA"
     gene            complement(21112..22473)
                     /locus_tag="VF517_15760"
     CDS             complement(21112..22473)
                     /locus_tag="VF517_15760"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8054443.1"
                     /translation="MASESQNADVLVVGAGIAGLTAAHHLREQGRDVVVLEADAVPGG
                     RVRTEEWDGCHIELGGVYMAARYDELMRLTAELGLEDELRDLPNAFRTAVRRDGDWSY
                     ADYAHTPNPFTRARDFARFSAVSWRDRASVAKLTGPMARVAARRARFYDVASGARVDR
                     AALQDVVAHDAARFYVSPLIEVFCGYRLEEIGLPMVVLAADPPGKALTPRAGMGSIPA
                     ALAARLDVRCGQRVTRVETGDGGVSVAATGPDGAELEYRATGAVVATPADVTLDLWPG
                     APAAARAFLASTEYSDGFVHFVRTREPFQKADPRGNDLYMEVIPPEPGRALHAVVFAN
                     FLAHDGGGLVALSASPDARARGDEELAAALEAELVELHPELSGAITGRRPVRAERVVP
                     RFPSGRARELAEFRSSLEPGPVQLAGDYLYGACMESAAQAGMDAAGRLPRGQSPAAAS
                     PSR"
     gene            22504..22986
                     /locus_tag="VF517_15765"
     CDS             22504..22986
                     /locus_tag="VF517_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054444.1"
                     /translation="MDGPSLERSLAAFAALSAMLLALDVRMRRTGGGGIVALEWAGSP
                     RRADAVLRRWGPAGRRAALASLVLEFGYAVSFSALLAAGRRRFAPASARTPAAALAPA
                     AGALDVVQTVALLMVLAGRRGTWPRVARWSSFTKFGVMGADVALIAVAAARPSDPSGS
                     "
     gene            complement(22964..>23276)
                     /locus_tag="VF517_15770"
     CDS             complement(22964..>23276)
                     /locus_tag="VF517_15770"
                     /inference="COORDINATES: protein motif:HMM:NF015668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054445.1"
                     /translation="PTPPGERADYCECGWPYNMLLPRATAEGMPFKLAVFATDTGRDS
                     VPHEDRCGSASFCGVRNRRYPDTRPMGYPFDMPFAGGVVDSLRAQPHAAVRDLTIRWD
                     R"
BASE COUNT         3111 a         8292 c         8533 g         3340 t
ORIGIN      
        1 cgcggcctgg agccagcggc ggcgctcgcc gccgctcgtg ccgagcgcgc cggctccgag
       61 caccaggtca cgcacgccga acatgcgggt cacgaacggg agctgggggt tgcggcgcga
      121 gtcgaggccc acgccgctga atgccttgcc tggtgccgcg agtgccgcca cgcccaccag
      181 gagccgggag ccgttcaaga gccggttgaa gtcgtcgcct gtcacaggat cgctatctcc
      241 gcgttgtcgc ctacaacgaa acggtacgcc ttcggcagcg ccgggctccg cccgatcgcc
      301 acgttgcgcc cgataagact tgcctctacc cgcccgtgga ggtctcgcac cgacgagccc
      361 gcgagcacga tcgagtactc gatctcggac ccgtggatgg tcacgtcctc gccgatggcg
      421 gtgtaggggc cgatgtacgc gtcggtgatc cgcgcgccgc gaccgatcac ggccgggccg
      481 cgcaccgtcg agcgttcgag caccgcgccc gtctcgatca ccacgcgtcc ctcgacgcgc
      541 gactcgatca gctcgccctc gatccgctcg ccgagatcgt cgaggatcag ccggttcgcg
      601 tcgagcatgt cctgcacctg gccggtgtcc ttccaccagc cgcgcacgat gtgcgggtcg
      661 acggtgaggc cggagtcgat catccgctgg atcgcgtcgg tgatctcgag ctcgtcgcgc
      721 cacgacggct cgatcgcgcg cgcggcctcg aagatcgccg gggtgaacat gtagaccccg
      781 accagcgcga ggtcgctctg cggctgctcg ggcttctcca cgagccgcgc cacgcgctcg
      841 ccgtccagct ccgctacgcc gtagttctgc ggatccggga cgggcgtgag caggatcagc
      901 gcgtcgggct cctgggagcg gaactgctgc acgagctcga cgatcccgtc gcgcaggagg
      961 ttgtcgccca ggtacatcac gaacggggag tcgccgagga agtcggcggc ggtgagcacg
     1021 gcgtgggcga ggccgttcgg cgcgtcctgc tcgatgtagg tgatcttcac gccgaagcgc
     1081 gacccgtcgc cggccgcggc ctctatctcg ccgccggtct cgggggcgat gatgatcccg
     1141 atctcctcga tcccggccgc ggcaagcgac tcgatcccgt agaagagcac cggcttgttg
     1201 gcgaccggca cgagctgctt cgcgcgcgtg aaggtgatcg ggcgcaggcg cgtgccgcgg
     1261 ccacccgaga ggatgagtcc cttcaggggg cccacctagt aggtcccccc gagcttggtg
     1321 tgatcccagc tcaccacgcg agtaggaaca aaccgcaggc ccactcgctt cgctgcctgc
     1381 cccgcgacca tgtcgcgcgt ctccggcggc agctcggtcc cgccggcgta gcgctcgaac
     1441 agcgccatgc cgtagccggt gaccttctcg gtgtcgtgct cgagctggac gtcgcattcg
     1501 agcatcacgc cgcgcagctc gtggtactgg atgccgtcct cgacctggag ggtggcgcgc
     1561 gggtcgcgct cgaggttctt ggccttctgc gacttggcgt aagtccagcc ggtcagggtg
     1621 gggccgtcct cgggcacgta ccagagcggc acgaggtgcg gccggccact cgggccgacg
     1681 gtcgcgacgc tcacgatcat ctgctcggcg aggaagtcgc gcagctcctc gtcgctcatg
     1741 cgtatctggt cgcgtcgcga cgccaaggcg gcgcaagggt atgcggtcgt gcacacaccg
     1801 cggcgggcgg cgggcatagt ctgccgcgtg tgcggagcga gcgcgaggcg cggatgcaga
     1861 acctcggcgg gcgcgtgccg tggttcggca gcgccgtgcc gcggctgcac gcgcggctct
     1921 acaggcggct cggcgggcgc ttcgtggggc gctggttcgg cggaccggtg gccgttttgg
     1981 agacggtcgg gcgccgcagc gggaggccgc gcgcggtgcc cgtgatctac gcgtgggatg
     2041 gcgaggacgt ggtggtcgtg gccgcgaacg cgggcaaccc gcggccgccg gcgtggtggc
     2101 tgaacctgcg cgagcagccg cgcgcgacgc tcgtgatcgg cggcgagcgc cggggcgtgc
     2161 gcgcgcggga ggcacgcggt gaggagcgcg agcggcggct ggcgatctac aagcgcgtgt
     2221 acgcgaaggt ggacgactac ggcgcgttca ccgaccgcga gttcccggtc gtggtgctgg
     2281 agccggagcc gtaggccgcg cgggcggagc cgggggcggc gcgccggtcg ccgagcggct
     2341 gcgcgcgcag ctcctctcgg gcgagccggc ccgcgatccg gtggcggtcg ccgagcggct
     2401 gctcgccatc cagggtcagg acgcgcgcgg attccggctc gcggtgcgcg cgcgcacgcg
     2461 cgggctcacg gcggcggacg tcgagcgctg cctgggcgag gagcgctcac tgctcgtcac
     2521 ctgggtgaac cgcggcacgc tccacctgat ccgctccgag gactatccgt ggctgcacgc
     2581 gctgaccacc ccgccgctgc tgaccggctg catgaggcgg ctcgcgcagg agggggtcga
     2641 gccgggcgcg gccgatcgcg ccgtggacgt gatcgagcga gccctcatcg aggagggccc
     2701 gctcgcgcgg gagcagctgc gcgaccggct tcgctcggcg gggctgcgca ccgaggggca
     2761 ggcgctgatc cacctggtgt tcctcgcgtc gctgcgcggc gtgtgcgtgc gcgggccgat
     2821 gatcggcaag cagcacgcct acgtgctggt gcgcgactgg ctcggggagg cgccgccggt
     2881 ggatcgcgac cgggcgctgg ccgagctggc gcggcgctac ctggccggcc acggacccgc
     2941 cggcgaccgg gacctcgcga agtgggccgg gctgccgctg cgcgacgccc gggccgggtt
     3001 ggaagcgatc gccggggagg tcgtggagcg cgaggacggc ctggtcgagc tcgcgcgcgg
     3061 cggttccggg cgcggcggag gcgcggcacc cccgcccgcg gagctaccgc ccccggtcct
     3121 cctcggcgcc ttcgacccgt gcctgctcgg ctgggccacg cgcgagccga tcgtcgggga
     3181 gcacgactcc gcgatcgtgt ccggcgggat cttccgcccg ttcgcgctgg tgaagggacg
     3241 cgtggcgggg gtgtggcggc tcgagcgcgg cgcggtgaag ctggagccgc tcgagcggat
     3301 cgcccgggcg gacgcagagg cgctcgacgc cgacgcggcc gacgtgaggc ggttcttgaa
     3361 atagcaccgc atcgccggcc ccgtcgggcc gcagctgctt cgcgggcccg acggctgtac
     3421 gccgatgaag tcgcgctcgc ggtcagattg ccaagcgcca ccgtgcgtca gaatgggctc
     3481 gtgcatcgcc tgacacgagt ttccttggct gggttccaga gctatcaggt ccgccaagag
     3541 atctcactcg acccggacct aacgttcctc gttggccgca acaacgtcgg gaagtccgca
     3601 ttccttcgag cgctgagggc ttttgctgac ccgacagaat acgtgcagcc tggtctggac
     3661 gtaacctaca cgtggcacgt aaccagcgat gtgattgtca atcagctgga tccaaagcta
     3721 ggtgctgcag tcaaggaaat tggatcctac ccagcgcatg agctcgtcgc gtcgtatgtc
     3781 gccgcacaga cgcctggcac tcagcaagcg gccatgtact gctatcggct tgaactgccg
     3841 gagctcggct ggcacgcaga aggacaggta ggatacaaag cagggtggaa ctctggaccc
     3901 tggaagcagt cgagtacagg ctttcaggaa ctcgaacagc tggtacggcg gtgggtgacg
     3961 gcggttgagc tgatagcgcc tcgtcgcgta gaccaaggac gtcgcggcct ctaccctcag
     4021 tcacgccttg cccctgaggc gcgcaacctg accgaggtcc tcttcgacct tcagttgaat
     4081 aagccgtcgg gaacctttcg ggacctcatc gcgttcatga aagaggcgtt ccccgaaatc
     4141 gacaccataa ccgtgcggac accgtcggat gagtcatcgc cgatggggga gatccacatc
     4201 atttacggca acacttcaca gtcggtaccg ctccgactgt gtgggaccgg tgtggagcaa
     4261 atgctggcgc tgggtaccgc cgtgctcaac gcaggctcgc cgcggctagt gctgatcgac
     4321 gaaccgcaag cctatttaca ccctcacgcc gagcgaagcc tgctctcact tgttgaagcg
     4381 cacccggagc atcaatacgt cgtggctact cactccgggt tgctactcaa tgcacggcca
     4441 atttcgcact cgagattgat tacgattgag aatgggtcca gccgtatttc ggatcctgac
     4501 agcgatagcg gcatccttac ggaactcggt atcggtccag cggacctctg gcttccagac
     4561 cgcctactgt gggtggaggg agcgtcggac gagacagtgt ttcgcacgct gctgaaggag
     4621 gaggtcgacc ctgcttcggc ggcaggactt cgtatcaggc ccatgcccga gtcctcccgt
     4681 ttcacggggc gtacgcgtcg ccacgcagat gcggcttacc gcttctgcaa ggaagtcgcg
     4741 gatgccgtga gcccattgtc tgttgagatg ctgtttctct tcgatcgcga cgagaaggat
     4801 tcatcgataa tggacgcgat tagtgaagca agcagtaatc gtgctcgttt tctcccagtg
     4861 cgagagatcg agaacctctt cctagacgtg gacgtacttc acgcggcggt aacccatcga
     4921 gccgagacac ttgaacaggc cccgccgtcg cgctcggaag tccagacgtt tcttgcgcag
     4981 cttctcgcag cggccggtga tcgtcggctg tttcccagcc ccccacgtca gccagagagc
     5041 gcggtggtcg atgtacgggg ctctgccgtc ctcgacgaga tgttctggcg gtacaccacg
     5101 agtcggtacg acaagatccg cgacgcgaag ctcctcaccg aactagccct tcaacacgcg
     5161 ccccatgtgc tggacccctt gcgggcagtc ttgaccgagt tcgccttaca gcgtcaaact
     5221 gtgaactagc gcgccgcggc gccggccagc gcctcggccg cttgcagcac tgcctcgcgc
     5281 gcgtcctcgg gctcgaacac gaccgcgtcg ccggcctcct cgaggatctg gcgcacgagc
     5341 cagtccctgc cggcgtagtg cacctcgacg atcacggccc cgtcgcgcag ctcctccacc
     5401 acgcggcggt cctccctcgc ccagcgcgcg cgctccggtg agatccacac gcgcgccgcg
     5461 cgcgaggcct ccacctcgcc ggtgcgcggc cacccctgca cgtcgggctc gatccccgga
     5521 cgcggctcga actcctcgtc gaggatctcc gcctcgcgga tgcggtcgag ccggaagctg
     5581 cgggtcgcgt cgcggtcgag gtcccagcag tgcacgtacc agccctcctg cccgttgagg
     5641 agctggtagg gctccacgcg ccgggtcgtg aactcgtcct cgttctcctt gtagtagtcg
     5701 aggcggagca ggcggtggtc ggcgatcgcc tggctgacga tgcgcgcgat ctcggagtcg
     5761 tcgcccgcgg cggtggtgat ctggagcccg tcggtggccg ggtcgcgccc gagcgcctcg
     5821 acgatcttcc cgcgcgccga cgccagcgag ccctgcggga ggtggtcgcc gatcaggtcg
     5881 atcgcggcca cgagcgcctt ggcctccagc ggcagcaggc gggccggctt ggcgaagttg
     5941 tcgccgtacg gctccgggtc gacctcgatc gtgtcgtcgt gcacctcggc gtagagcacg
     6001 tagctgccgc caccgaagtt cacgacgttc agcacgtcga tgtcctcgcg cagctcgatg
     6061 tcggtgagct ggaggctctc gcgcacctgg cgcgtgtcga gcttcgagcc ggtgcgggcg
     6121 gcgtcgatca ggatccccgc gagcgtgacc aagcgggcga agcgctcggg gcggatcagg
     6181 gcctcgccgc ggccgctgtc ggcctcgggg gccgccacca cgggcttccg gcgcgccgcc
     6241 ggcgccggct cgagcggagt ggagtggcgc tcgacgatcg tgcggaccgc cgcggaggcg
     6301 cgctcggcca gctcgggcgg gccgaggatg cgcgcgcgct cgcccaggcc gagcagccac
     6361 gagacgatct ggcgctcgtc gccgtaagcg gtctcgaaga ccttgccgtc ctcgggggcg
     6421 tcgtcgacct cggcggcggg cacgatcgtc ccagcctcgc cgaagtggcg ctcgacgagc
     6481 cagtcgatct gcggcgcgac ccagatccgc gccgtgccga tcgtgtcgcc gagctgccag
     6541 tcggtgcggg tggcgtagcc gcgcgggtcg aagtcctcgg gcggcgggaa gtcgtgctcg
     6601 gccttcgacg agtagccgac cttgccttgt atgcgcgaga gccggaacac gcgcacgtcg
     6661 tcgcgctcgt gcgagtgccc gacgaggtag aagtgaccgc cgcggaagag caggtggtac
     6721 gggtccacgc ggcgctgctc ctgcgtgtcg cggcccatcg tgtagtagtc gaagacgatc
     6781 gtcttgcggc ggaagatcgc ggtctcgacc ttggcgagcc gctgcgagag ctcgcgcccg
     6841 cccgcgcccg ccgtgacgcc cagcgcgacc tgccggttct ccggcgccga gagcggcgag
     6901 ggcttgcccc acgagagctg ctggagcgcg agccgcagcg gctcggcgta ggcgaactcg
     6961 ccgtcgagca gcgagagcgc cgtgcgcagc gcgccgagct cctcgtcggt gaacttgatc
     7021 gacggcaggt agaagttctg cggcggcagc gagtagttct cggcctcgta gtagccctcg
     7081 gcgggcttgt cgaccttcag ctcgataccg agcgactcga gttcggcacg gtcggcgtag
     7141 aagcggcgcg cgaacgcgtc gtcgttcatg cccgagtagc cctcgacctc ctgcttgatc
     7201 tcaagcgcgg tgaccgggcg gcgctcggcc atgaggaacg agatcagcga aagctgacgg
     7261 atgagcttct cggtgtcctt ggccatgcgg gatcacctta atgagccgcg tcgcgaagaa
     7321 ttacgccccc cgcgccggaa gtcgggcggt ttgccgcact ttgcggccta gtctccgcgt
     7381 tccgacggcg gtttgccttg cgaggctgcg aaaaccaagc agcgcaagga cgcagggagc
     7441 gtggcgtgcg ctagcacgcg agcgaccgag gacgccgcga tgcgccggtt tgcagcagcc
     7501 gccgctaggc ggcttcgccg agtcccgcga taccgcgagc gccgaagtac gccgggcccg
     7561 acagggcgcg gtcgaagctc gaccaccgct cgccgttctg gaggcccttc tcgatccggt
     7621 cgaacgagcc caggcggccg cccagattgt cgatcatgtg cacgagggtc gcctcgcgcg
     7681 tgcacggcac gaccgggctg ccgtgctcga gctgaccgtg gtgggagagg atgatgtgga
     7741 gcaccgcctc ggcgcggtcg ggcgggaagt cggggatgtc ctcgatcgcg cggcggaccc
     7801 ggtagtagcc gagcggtatc tcgccctgga ggcggccggc gtcggtgagg tcgatggcgc
     7861 cgccctccat cgcgtacgcc tcgatcttcc cgatgtcgtg cagtagggcg ccggtgaccg
     7921 ccacgtcgcg gtcgagcccc gggaacgtgg acgacatcgc gctcacgccc tgcgccaccg
     7981 acaggcagtg ctccatcagc ccgtgcctgt aggcctggtg gtagtgcttg gcggccggcg
     8041 cgtcacgcca gcgctcccac accgcgcagt cctcggcgaa gaagccgtcg agcagcaggc
     8101 gcaggtgggg atcctggatc gtgccgatca gctcccgcag cgcggccacc atccggtcgc
     8161 acgactcgac cgggccgtcg tgcagatctg cgggctcgta ctcgcccgcg cgcgcggggc
     8221 ggagggagcg gatcgtgagg caggcgccgt acttcgcgtc gcactcgtag gccccgttcg
     8281 cgattaccac ggcgccgagc acggccacct cgcaggcgga ctcgaccccg tcccacacca
     8341 cgccctcgac cgtgccggtc acgtcgccga gcttgagctt caggaacgcc tcgccgttgc
     8401 gcttcgtgcg cctggtgacc tcgcgcacga cgaacggcga gacgacgcga gcgccgtcgg
     8461 cgaggtcgcg gacgaagacc ttggcgggca gcgcggcggc ggccggcggc agcccgccgg
     8521 ccagcggcgc ttcctcgatg aatgcagggc ttccatcgag ctgcggctgt gcgggaccgg
     8581 ggtccatggg agggtcaagg tagatcgagc gccggacgac tcggcctgat cgttagggtc
     8641 cggaggcgat gcccgacgcg cccgcagcag cctccgccgt gctcgtcgcg ggcggcaccg
     8701 gagccctcgg cacggccgtg gtgcgcgagc tgctcgacgc gggccgcccg gtcaccgcca
     8761 cctggctcgt cgagcgcgag cgcgagcggg tcgaggcgga gctcgggggc gacgatctgc
     8821 tgaccctcgt ccaggccgac ctgatggagc tcgagggtgc ggaggcggcg gtggcggcca
     8881 ccgacgggct cggcgcggtg gtgaacctgg tcggcggatt cgcctccggg ccgctcgcgc
     8941 acgagaccca ccccgacgag ttcgaccgca tgctgcgcct gaacgtgcgc cccggctgca
     9001 ctctcgtgtg cgcggcgatg ccgaggatgc tcgaggcggg tggcggtgcg tacgtcggcg
     9061 tgtcggcgcg cccggcgctc aggccgttcg cgggcgccgc ctcgtacgtc accgcgaagg
     9121 ccgccgtgct cgccttcgtc caggcgctcg acgccgacta ccgcaagcac gggatccgcg
     9181 ccaacgcgat cctcccgagc gtgatcgaca cccccgcgaa ccgcgccgcg cagccggacg
     9241 cggaccactc cgactgggtc cagccggccg agatcgcacg cgtggtgagc ttcctggtgt
     9301 cgggcgatgc cgccgtcacg tcaggcggcg cgatcccggt gtacggccgc gcctgacggg
     9361 tacagcgagg gcatgacgac gatacccagc caaggaatcc tcagcgagga caagcaggcg
     9421 gagcgcgacg agctgatcgc cctcctaacc aaggcgtact ggatggagct cgagacggtc
     9481 atgagctaca tctcgaactc gacgaacccc gacggcgtgc gtgcccagga gatcatcgag
     9541 tcgctcaagc aggacgtcca ggaggagctg ggccacgccc aggagttcgc caaccggatc
     9601 aaggagctct acggcgtggt gccgggctcg ctcgacttcc aggccgagca gagctacctc
     9661 cagccgcccg agcagcagac cgacatcgtg cacgtcataa agggcgtgat ccaggccgag
     9721 tccggcgcga tcgagcacta caaccggatc atcgagttct gcgagggcaa ggacccggtg
     9781 acgcaggaca tggtcatcgc gatcctgcgc gacgaggagg gccaccggcg gctgttcgag
     9841 ggtttcctgc gcgagtacca ggtggagggc cgggcctagc gggtccacgc cgggacgcgg
     9901 tggccggccc gggcggcgct cagtcgcgcg ggctgtagcg cttcagcggt gtcggcacca
     9961 gcgggatcgc cagccgcttg ccggcggcca tcgtggccgt gaacagcgcc gtgtgcgcgg
    10021 gcgtccaccc aaggccgtag cccctgcgga tcggctccgg cagcagcccg gcgatcagga
    10081 agttgacgag cggccagccc tgccgcaggc cgagcggcag cggcgggccg aggaccgtgc
    10141 cgcgggccac ctcgcgcgcc tgctgggtca cggtgaccac gtcgctcgcg accatgtcct
    10201 cgacgtagtc gtccaggtcc gcccaggttg cggggatcgc ggccgcgggg agggagaaga
    10261 gctccgccac ggccttcatc tcctcgtggt aggcgtcctt gtcgcgatcg gacatccggc
    10321 ggacgagcgc ctcgtagacc acgatcgcgc cgtggacgaa gttggcgtgg atccagagca
    10381 ggagctcggg gtcctccgcg ctgtagcggg tgcccgccgg gaacgacccg agcggctcgg
    10441 tcagctcgcc gttgacccgg gtgtgcatcg cccgcacgcg ctccccgacg gcctcggcct
    10501 gctcgcgcgt gccgaagatc gccatcatca tcgagtgagc cgtccgccac agcctccccc
    10561 acggctcctc gtcgtaggcg gagtgcccca ggaagccggc cagtgcgagc gggtgcgcgg
    10621 cctgcatcag cagcgagcgc ccaccgctaa gcagcagcac gcgctcgcgg tggaccgtcc
    10681 acgcgacgct gccgtcaccg aagctcccgc ccgcgccgcg cccgcgcagg ccctcgatgc
    10741 gcgtccgtcc tgcgactgtc gtggtcacca gatcgagcct aggcgggcgg acaccggggc
    10801 gtcatccctc cattgggggg gttagagggg gacggctgta cgatccggcc gatggggggc
    10861 atgagctcgg tcgtgaggcg atcgtcggac cagacgctgg cgcgcgtgtt cgcccgccgg
    10921 ccggcccggc ggctgctcgt cgcgacggtc gtgcgcttca tcccacggcg cgcgcccgag
    10981 aacctcgacg gctgcgtgct ggtcgagctg gtctcgcccc ccacgcggcg gcgcgagaag
    11041 cgcagcgagc cgtgggtgtt cgagttcagc gagggcagcg cgcgcgcccg ccgcggcgag
    11101 gtgagcgagc cgcagctgag cgtgtcggtc gcggtgccgg atttcgtgcg gatcgccgcc
    11161 ggccagctcg acccgatgag cctcgtcttc gacaagcgga tccgtatgaa gggcagcttc
    11221 gcgctggcgc ggcggctgtt cgcgctcgcg ccgggggacg aggcgcaatg acgcagaccg
    11281 ccgccgagac cgaggcgccc gtcgtggtcg agaccgtcgg gctcgtgcgc gtgtacgggg
    11341 agggagacaa cgcggtccag gccctgcgcg gagtcgacct ccaggtgcgg cgcggggagt
    11401 tcgtcgcggt cgtcggcccg tccgggtccg gcaagtccac cctcctgcat ctgctcggcg
    11461 ggctcgaccg ccccgacgcc ggggaggtcc tgctcgacgg gacctcgctc gcttcgctgc
    11521 ccgacaagga gctcggcctg atccggcgcc gccagctcgg cttcgtgctc cagttcttca
    11581 acctgctgcc cacgctcgac gccgccgaga acgcggcgtt cccgctcagc ctcgacgggg
    11641 tgcccgacgc catcgaccgc gcccgcaccg cgctcgggct cgtcggcctg gccggccgcg
    11701 aggaccaccg gccctcgcag ctctccggcg gcgagcagca gcgcgtggcg atcgcgcgcg
    11761 cgctcgtgac ccgcccgagc ctgctgctcg cggacgagcc caccggcaac ctcgactcgc
    11821 gcacgggtca ggagatcgtt gacctcttgc ggcgcaccgc cgacgagggc cagaccatcg
    11881 tgatggtcac gcacgacctg cgctggaccg gcgtggcgga ccgcgtggtg cgcatcgagg
    11941 acgggcagat cgcgacctcg gacgagaccg cgtccgtcgc cccttgaggg ccttcgccgg
    12001 cctcttccgc cgcatcaacg cgcggcactt cggcgagcag cgcctgcgca cgctgatcac
    12061 gatcggcggg atctcggccg gcgtggccct gacggtgtcg atcgcggtca tcaacgccac
    12121 cctccaggtg tcggtgaagg ccaccgcgcg cgatccgacc ggcgccgccg acgtcgaggt
    12181 gcagccggcg aacctgagcg gcctgccgca gtcggcggtg agcagcgtgg cctccgtgcc
    12241 cggcgtccgc gacgcggtgc cgttcacgcg cgtggccgcg cgcgtgaagg gcgattcggg
    12301 cagcgtgcgc acgatcgtgc tcggcggcga ccccgggccc accgccaggc tggcgagccg
    12361 catcgagcag gcggactcgc tgaagatcac ccagcgcggg tcgctcagcg gcggcgtgct
    12421 gctgacgccc gaccttgccg acgagatcgg cgccggcgtg ggcggccgcg tcgaggtcga
    12481 gacgccgcac gggccggtcg agctgcccgt ggcgggcacg gtcgagggca gcgcggtcga
    12541 gcgcttcaac gcggggcgcg tggccttcat gcacatcgcc acggcgcagg ccacgttcga
    12601 ccgccgcggc accgtggacg cgatctacgt cgtcgccgac ccgggcacac cgataccgca
    12661 gctccaggac gcgatccgcg agcgcctgca cggagcgggg atcgtcgggg acccgggcga
    12721 ggtgagccgg gggttcgaga acaccctcgg cccgctcgcc gcggccggca ccatctcggg
    12781 tctcgtggcg ctgctggtcg cgctgttcat cgtctacaac acgatgtcga tggcgttcgc
    12841 cgagcgccgc tgggaggtgt cgatggccct ggcgctcggc gcctcgcgcc ggcagctcgc
    12901 gggggcgttc ctgctcgagg cgctggtgat cggcatcatc gcgtcggcgg tcggggtgtt
    12961 gaccggaacc gcgtccgcgt acgtgatggc cgagcgcgcg atcgaggact accggggcat
    13021 cacggtcggc ctcgcgacgc cgccggtcat acacgcgcgc gacctgctcc tcgggggcct
    13081 cggcggcgtg atcgtgacgc tgctcggcgc cgcgatcccc gcgcgccggg tcttcggcct
    13141 cgcgccgatc gagtcgctgc ggccggaggc ggcacacgag tgggcggcga ccgtccgcgg
    13201 cagcagctgg acccggcgcc agatcgtggc gggcctgctc gccgtggcgc tcggcacggt
    13261 cgggctgctg ctctcgatcc gcctggtgca ggaggactgg ctcgccacgg tcaccaccgc
    13321 gctctggctc gcgggcgtgg cgctgctgct gccgcacgcg ctgccgatcg tcggccgcgc
    13381 actgcagccc gcgctcgcgc ggaccttcgg cgccctcggc cggctggccg gcgaggcgct
    13441 caccaagaat ccggggcgga cggcgctcac cacgggcgcg ctcatcgtca gctcgtcgat
    13501 ggcaatcgcc gtggccagcg ggctcgactc ctacgagcag cgcttcgcga acaccgccga
    13561 gggctggttc gggggcgacc tgtacgtggt ggccgggaag agcgacaaca gcgtcggcgc
    13621 cgaccagccc gtggacccga gcgcggcgga tgcgctgcgc cgcgtcgagg gcgtgcgcgc
    13681 gctctatccg cagcgctacg cggtgacgcg cgtgggcggc gagcccgcgc tgatgtacgc
    13741 gatcccgatc gcgcaggccg cgcgcgaggg caccacccgg acgctcagcg agtcgggcgc
    13801 cgaccaggac gcgctgatcc gcgggctcgg gcgcggcgag gtcggggtgt cgcgcttcac
    13861 ggccgcgcgg ctagacttga aggcgggcga cacgatcccg atcgccacgc ccaccggcac
    13921 ccgccgcttc cggatcgcgg ccctgttcaa ggacctggcg ggcttcgaca cgatccagat
    13981 cgagcactcg acctacgcgc gctactggaa cgaccgcaag gtggaccgct tcgcggtact
    14041 cgcgcagccc ggcgcgaacc tcggaaagct gcgggccgac ctcgggcggg cggtggaggc
    14101 ggccggcctc gacggccggg tgctcgagca gcagcaggcg gtggaggagc tggtcggggg
    14161 cgtcgagggc ctcgtatcgc tcgcgcgctc gatccagctc gtggcgctgg tggtggccgc
    14221 cctcgcgatc gccaacacgc tgttcgccgc cgtgatcgag cggcgctggg agttcgggct
    14281 gcagcgcgtg ctcgggatga gccggtcgga ggtggcccgc acgctgctgc tcgaggccgg
    14341 cgtgatcggt ctgatgggaa gcgtgatcgg cgtggtgctg ggggtcgcca tgggatacgt
    14401 gatggtgcgc gcgatgacgg ccggctactc gtgggacatc ccgttcgcga tgccgtggga
    14461 gctgatcgcg ttcgggctcg tcggcggggt ggcgatcgcg gcggcggcgg gcgtcacgcc
    14521 cgcgcggatc gccacacgca cgtcgatcgt cgcggcgctg cggcgctgat cagcccgggt
    14581 cgagcgactt cagctcggcg tggtacctca tgcggaagcg ctcgcccgcg attgcgcggg
    14641 tctgcgtgac ggtctcctcc acgaggacgt tgtcgtccga gacccagcgc tccgactcct
    14701 cggtcgcctt cacctcgccg cccgccatcg cgagcgtgag ccgcacgccc acggcgcgca
    14761 cgtcgcgccc ggcgaccgtg atcgtgcggc ggcctgcgaa gtcggacctg tagcgaccgc
    14821 tcgccggtcc ctcgaagctc accgagccgc gctgcgcctc gagcgtgccg ttgaacgtgc
    14881 cgctcccgga gggctcgttg gtgtcgagcg ggatcggcgg gctcaggcgg aactccgcgc
    14941 gctggccacc cccctgcgac acgaactcgg acgcgtacgc gctcgacccg gcgaccgcgg
    15001 ccagcgcggt gtcgccgaaa cgcggcgagt agagccgctg cacgcggtag cagcgcagcg
    15061 cgccggcggc acgcgcgggg gtcacgatca ggcgggtctc gtgcggcagc ggctggctgc
    15121 ggccctcgag cgtctgcgtg ccctcggtcc ggaagcggta ctcgcccggc ggggcgacca
    15181 gctcggcgcg gctctcgccc ccgcccgcca cgcggccgac ggccgcgcac tcctccgccg
    15241 aggggcgggt cgggggatcg ggccggtcga cgttccgcac cggggcatcg ccggagccgt
    15301 cgtcaccgcc gcagccggcc acggccagcg cgatcagcag cgccggcacc agccgcgagc
    15361 ggacggtcac gcgttggcgg cgaggctcgg ctcgccggcg ggcccggccg cggtcctcac
    15421 gcgctcgagc gccagctcgg ccaccgccac gagcgggtcg ccggcgccgt tgagcgccag
    15481 ccgctcgacg atcgcggtgg cactcgccac gtgctgctcg cagtcgcgca ccgccgcctc
    15541 gatcgccccg ctctcgcgga tcgccgccac ggcctccggc aggtcctcct tctcgatccc
    15601 gcgcgcgagc atgcgcttga gtcgcgcgtc gcgctggagc ccgtagacca cggggagcgt
    15661 gtagatgccc cagcgcaggt cggtgcccgg ccgcggcgcg gtctggtcgt cgcccgcgag
    15721 caggcgcaca acgtcgtcgg ccacctggct ggccataccg acgtgccacc cgtagtcggc
    15781 cagctccgcg acctggtcgt ccccggcgag cgcgagctcg gcgccgagcc gcgcggccgt
    15841 cgacaccacc gcgcccgccg tggccgccga tgccgcgaag tagcgctccg gcgtgcgctc
    15901 gaggtcgtag cgatcctcga cgtcgagcat cccgccctcg cacatgctgc acgccgcccg
    15961 ggccaggccg ctgaccacgt tctgaccgtg ctcggacgcg gccagcgccg cgcggctgac
    16021 cgcgaagtcg ctgatcagga cggccatcgc gttgttgaac cgggcctgct ggttgcgcac
    16081 cgggtcggcc acgagcgtgg atatccggcc gacgatcctc acgcactcga tcgccagcgc
    16141 cgcgggcagc acctcgcccg gcgtgccgcc gcaggcgcgg ttcgcgagca gcagcacgct
    16201 gccgtagagc ggccccgcgt gcgcgcgcag ctcgcggtgg agcagctccg acgcgaacgg
    16261 gaagcgcgtg cgcagcaggt cgagcgtccg gttgttcagc tcgtccaggt cgtccgcgca
    16321 ggactggtcg aggtcggcga gcgtgcggcg gacgggcgtg cccgtgagcg tcggctcctc
    16381 ctcggcctcg agggccatcc gcgcgatcga cttgacgaac ggcaggcgcg tgttcccgcc
    16441 cagctcgatc tcgcccttca tcagctcgcg cccgtagcgc aggccgccgc gcgtgacgag
    16501 cgatatgtcc tgcccgagcc gggggaagcg gcgcgccatc agcaggccgg ggtctggcgc
    16561 gcggtcgccc cgccgcgcgg cgtcgtgccc cacgttcgcg accagctcgc cgccgaacag
    16621 cgcgtaggcg atcccctcgc cggtgatcgg gtcggtcgtg gacgcggcgt ccccgacgaa
    16681 gagcatgttc cggagctggc agcgctcgcg ggtgaagttc gtggccagcg gcgagccgag
    16741 cggctcgccg gtcggctcca tgtcgcccac gcgccgcccc gcccggagcc gcaggtcctc
    16801 cacgaagcgg ctcagcacgg tgcgcagcgg cggcgggttg tccacgccgg gcacccgcca
    16861 cacccccacg ccgacgttgg cgaggtgctc gctgaccggg aacacccagc cgtagccgag
    16921 caggggcacg ccctcgtgct cgaccggaac gtagacgtcg aacgacggct cgagccggcg
    16981 ctcggtccgg aagtactggc gcacgccgta ggcgaccggc acgttcgggg gccgcgtgaa
    17041 gcccgcctcg cgccgcatgc gcgaggtggc gccgtcggcg gccaccacgt agcgcgccgc
    17101 gacctgcgcc tcgccgtcgt cgccgcgcac gatcacgccc tcgaccccgt cgtccacgat
    17161 cgccggcccc tgcgcgcggg cgcgcatgaa gcgcgccccc cgcgccagcg ccgcctcgcg
    17221 cagcgcgtcg tcgagccggc cgcgcggcac gcagcaggcg ctctcgccgc gccgggcgcc
    17281 gaagtcgcgg aagcgcgagt ggcggtagtc gagcactccg cgcaggcccg cgatcgggta
    17341 gctctggtcg atcaccggat cgaggccgta gcggcgcagg aaggccaccg cggacggcgt
    17401 caggccgtcg ccacacggtt tctcgcgcgg gaacgagccc tggtcgatca gcagcacgtc
    17461 gtggccgagc tcggcgagcc tggccgcgca cgcgctgccg gccgggcccg ctccgacgac
    17521 cgcgaactcg gcctcgtcga cgaccgcgcg cgcgccgctc acgccgtccc cacgggccgc
    17581 gcgcgggccg ccgtgtagtc gacgagcgcg cgcagtgacg cgccggcccc cggatccccg
    17641 gactcgagcg acgccagcgc ggcctgggcc gcttcggcgt ggcggtcgca gtgcgcctga
    17701 gcgcgctcca ggccgtcggt ggcccgtatc gcgtcgatga cgcccgctag gtcgtcgtcc
    17761 tcgacgccct cggccaggcg cggggcgagc cccgggtcct cctccatcgc gtagatgacc
    17821 ggcagcgtga agacgccgag ccgcagatcg ctgccggcgg gcttgccgat cgactcctcg
    17881 ccgcccgtga cgtccagcat gtcatcggag atctggaacg ccatcccgag gtcgtgcccg
    17941 aagctcgtga gcgcggccac caccggctcg ggcgcgccgg acgccttcgc cccgagcgac
    18001 gaggccagcg agaagagccg ggccgtcttg cccgcgatcg cgtcgaagta gctctcggcg
    18061 gtgcgggcga cgttgccgag atcctggacc tcgagcatct ctccgtcgca gatggcgcag
    18121 gcggcctccg cggcgtcgcg ggcgaactcg gtgtccgcca cgagctcgac cgcccgcgcg
    18181 cagagccagc cgcccgcgta ggcggcggct gggtgcccgt actcggccgc gacggtcgtg
    18241 atcttccgcc gcatctggcc gtcgtccacc acgtcgtcgt gggcgagcgt ggcgtagtgc
    18301 agcagctcgg tcgcggtcgc cacgcgccgg accacgtcgg agtccggttc gggcccgtag
    18361 cgcgcggcct cgagcacgat cgtcgagcgg agctgcttgc ccttcgtcgc gagcaggtgc
    18421 cggcaggcgt tcgtgagcgt ctggccctcc ggtaggccgg cggccacgcc ctcgatctcg
    18481 tcggggcgaa gcgtcgcgat ggccccctcg aagtagctgc ccacgtaccc cccgtcgtcg
    18541 ctcgcgtccc tccgggcctc cccggcacga ggtagtcgga tcctacgcgc cggggccgcc
    18601 cggctcaagc ggtactcgac ggaagcgcac cgggaagggg tgccggagcg gtcagcactc
    18661 acttgcgcgc gggcgcgcgc cgtggcaggc tgtgcacggt cgacgggggc agtgcccgtc
    18721 cagcatcggg gggtgtcgtg gagagcagtc agagcttcgc cggcgggctg aaggcggccc
    18781 gggcgcacat cggtgtcatc acgcgcgacg tgtggccgcc gcgcacggcg cttctcaacc
    18841 tgcgccgcgg cctgtggccg ccgagcgccg ccgtcgccac gttccggcgg gcgcgcgagg
    18901 tctggccgcc cctgctcgcg atcgcgaaga tgtcgaaggt caccttctgg ttcatctggg
    18961 tcacgccgct gatcttcggc tacatcgcct cggcggacag ccgcggctcg gctcaggacc
    19021 tgatctggtt cgtgctggtg ttggccggcg tgtgcttcgt ggagagcatc aactgcatcc
    19081 acaacgagct gatggaccag gaggaggacg ccgccaacca accccggcgc gcgacgctga
    19141 tcgcgtcggt cggcgagggg ccgctgtggc tcctcgtgct cgtggggtac gcggtcgtgg
    19201 ccatcgggat cgtcccgatc gtgctgttcg tcggcctcga cgtggccctc ctgatcacgg
    19261 ccgcgtcggt ggccccggtc ctctacaacg cgggaccgcg gctgaagcgc cgcgcggtga
    19321 tggcggagct cgacatcgcc tgggccgtgt tcttcacgta tctcaccgga tggtcgttca
    19381 accagccgat cggggacgtg ccggcgatca tctggctgct cacgttcttc ttcgggatca
    19441 cgtcgttcct caaggacatc ccggacatca ggggcgacca gcaggtcggc gccggcgggc
    19501 tgttcagcat caagcgcgac aacctgagga aggtggccac ggcgttcgtg tacgtgagcc
    19561 cgttcgccgt cgtggcagcg ctcgtcgcgg tggacgagct gccggaccgg atgctctggc
    19621 tgctcgcgct gctgccgctg tcggtgtggc tgatggtgat cggtgagcgc gtcgcgtcgc
    19681 tgggcacgct gatcgtggcc tacgagtcgt cgttcctcta cgtgcacgtg ttcttcctgg
    19741 tgatgttcgt cctgtacacg ccgacccttg cggcggcgat cgcggcagcg gtgctgttcg
    19801 ccgcgcgggt cctcgtgctc tacctgggtc tcgcgccgcg cttcgtggag ccggacttca
    19861 actggcgcac gtcgttcggc gcgctggtgc ggggggcgta gccgtggcgg cggagcggca
    19921 ctacgacatc gtggtcatcg gggccgggct cgcgggcctg gcgggcgtga tcgccctgcg
    19981 cgacgcgggg ctgagcgtgg ccgtggtcga caacatcgcg aagatcgacg ggtcgcggat
    20041 gaactggggc cacgacctcc agccgaacgc gctggccgcg ctcgagtccc tcggcctgct
    20101 ggaccgggtg agggaactcg gcgcctggca cgacgcgtac cagctcgaga agctcggcgg
    20161 cgggccgctc tcgcactggg actactcgat gctcgaccac cgccacccgt acgccgtgtg
    20221 catcaggacg cacgtgctgc gcgagctgct gcgcgaggcc acgcgggagc tcgacttcgt
    20281 cgacctccac atcccggcca cgtacacggg gctcgagcga accgagggcg gccacgcggt
    20341 cacgatcgag accggcgagg gggcgaccga gcgggtgcac gggcgcctcg tggtggcggc
    20401 ggacggtccg cgctcgcgcg tgcgcgaggc ggccgggatc agggcgcgct tcaagcgcgg
    20461 caagcgccac tggctcgacg tgatcatgga caaccccggc gaccggatca aggacggcta
    20521 cgtctacttc gggcgcggcg agtacctcgg gatcgtcccg acgcgtcccg gcgagctggt
    20581 cgcgttccac ctgacctcga cgccgaacct cgacgcctac aagcagatga tcgggggcat
    20641 cgacggcttc cgcgcgcgct atgcggagat ggcgccgatc ctcgccgagc cggcgcagag
    20701 cgtggactcg ttcgaccgca cctcgcacac gctcggccac agcatgcgcg ccgagacctg
    20761 ggtcgacgac ggcctgctgc tgctgggcga ctcgggcgtg acggtgaacc cgatcacgag
    20821 ctcgggcgcc gggctggcgc tcgaggaggg tgtcgcgttc ggcgaggtgg tcagcgagtg
    20881 cttcgcgaag ggcgacctct cggccgcggc gctcgcgccg tacgaggagc gctgccgccc
    20941 gcgcgccgag aagatgcagg acatgggcga cacctgcacg ttcgtgttcg gttcgcggct
    21001 gccggtggtc aacgggctga agctgcggat gctcagccgg atcgaccgcg acccggcgat
    21061 gaagcaccgg atcctcgcgt acttctccgg catggagccg gacccgatcg gctaccgcga
    21121 cgggctcgcg gcggccgggc tctggccgcg cggcaggcgc cccgcggcgt ccatgcccgc
    21181 ctgagccgcg ctctccatgc aggcgccgta caggtagtcg ccggcgagct gcacgggccc
    21241 cggctcgagg ctcgaccgga actcggccag ctcgcgcgcg cggccggagg gaaagcgcgg
    21301 cacgacccgc tcggcgcgca ccggccggcg ccccgtgatc gcgccgctca gctcgggatg
    21361 cagctccacc agctcggcct cgagcgccgc ggccagctcc tcgtcgccac gagcgcgcgc
    21421 gtcggggctc gccgataggg cgaccaggcc gccgccgtca tgcgccagga agttcgcgaa
    21481 gacgaccgcg tggagggcgc ggccgggctc cggggggatc acctccatgt agaggtcgtt
    21541 gccacgcggg tcggccttct ggaacggctc gcgggtgcgc acgaagtgca cgaagccgtc
    21601 ggagtactcg gtggaggcga ggaacgcgcg cgcggccgcg ggcgcacccg gccacaggtc
    21661 gagcgtcacg tcggcgggcg tggccacaac cgcgcccgtc gcccggtact ccagctcggc
    21721 cccgtcgggg ccggtcgcgg cgacgctcac cccgccgtcc cccgtctcca cgcgagtgac
    21781 ccgctggccg cagcgcacgt cgagccgcgc cgccagcgcc gccggaatgg agcccatccc
    21841 cgcgcgcggc gtgagcgcct tgcccggcgg gtcggcggcg agcaccacca tcggcaggcc
    21901 gatctcctcg agccggtagc cgcagaagac ctcgatcagc ggcgagacgt agaagcgcgc
    21961 cgcgtcgtgg gccaccacgt cctgaagcgc cgcgcggtcc acgcgcgcgc cgctcgccac
    22021 gtcgtagaag cgcgcccgcc gcgcggcgac cctggccatc gggccggtga gcttcgccac
    22081 cgaggcccgg tcgcgccagc tcaccgccga gaagcgcgcg aagtcgcggg cgcgcgtgaa
    22141 cgggttcggg gtgtgggcgt agtcggcgta gctccagtcg ccgtcgcggc gcaccgccgt
    22201 gcggaacgcg ttcgggaggt cgcgcagctc gtcctccagg ccgagctcgg cggtcagccg
    22261 catcagctcg tcgtagcgcg cggccatgta gacgccgccc agctcgatgt ggcagccgtc
    22321 ccactcctcg gtgcgcacgc gcccgcccgg cacggcgtcg gcctcgagca ccaccacgtc
    22381 cctcccctgc tcgcgcaggt ggtgcgcggc ggtgagaccg gcgatgcccg ccccgaccac
    22441 gagcacgtcg gcgttctgcg attccgaggc cacggagcgg gcgcgagtgt accttggcgg
    22501 tccgtggacg gaccgtcgct cgaacgctcc ctcgccgcgt tcgcggcgct cagcgccatg
    22561 ctgctcgcgc tcgacgtgcg catgcggcgc accgggggcg gtggcatcgt cgcgctcgag
    22621 tgggccggca gcccgcggcg cgccgacgcg gtgctgcgcc gctgggggcc cgccggccgg
    22681 cgcgcggcgc tggcgtcgct ggtgctcgag ttcggctacg ccgtgagctt cagcgcgctg
    22741 ctggccgcgg ggcggcggcg cttcgcgccg gccagcgccc ggaccccggc cgcggcgctg
    22801 gccccggcgg cgggcgcgct cgacgtggtc cagaccgtgg cgctgctgat ggtgctcgcc
    22861 ggccgccgcg gcacatggcc gcgcgtggcg cgctggtcgt cgttcaccaa gttcggcgtg
    22921 atgggcgccg acgtcgcgct gatcgcggtg gccgccgcca ggcctagcga tcccagcgga
    22981 tcgtgaggtc gcgcaccgcc gcgtgcggct gcgcgcgcag cgagtccacc accccgccgg
    23041 cgaacggcat gtcgaacggg tagcccatcg gccgcgtgtc cgggtagcgg cggttgcgca
    23101 cgccgcagaa gctggccgag ccgcagcgat cctcgtgcgg cacgctgtcg cgccccgtgt
    23161 ctgtcgcgaa gaccgccagc ttgaacggca tcccctcggc cgtcgcgcgc ggcaggagca
    23221 tgttgtacgg ccagccgcac tcgcagtagt cggctcgctc gccgggcggg gtgggg
//
LOCUS       DASVPL010000284        22254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_150168, whole genome shotgun sequence.
ACCESSION   DASVPL010000284 DASVPL010000000
VERSION     DASVPL010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22254
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150168"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VF517_15775"
     CDS             <1..305
                     /locus_tag="VF517_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8054446.1"
                     /translation="NATRGCEDQLGRVEQVRREFPGVAFVGVVSKRSRAEAEALARAN
                     GWGFPVVVDRDAQLFNLYGIGDCPTTIFARRGGIANRSVRGELGAARLRAELAEIR"
     gene            302..955
                     /locus_tag="VF517_15780"
     CDS             302..955
                     /locus_tag="VF517_15780"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEX8054447.1"
                     /translation="MTEEPRLEPGWIAEELRIEFPELRLVTTTIDGGPARSPNAVKDR
                     LRVLSNRYTGQKAIALRQQPIPWAYRVFFRQVGIDPDDHRTPVEAYALERMRAGGFQS
                     QNLLLDALTIATVETGVPVVAFDETTLDGPLGLRLSEPGELLGGDGRPLSSRQVVIAD
                     AERSVAVLFADFAEGVEPRSRTRRLRLASVQVKGVPDVSVEEALWTVAETVTGTELP"
     gene            1060..1527
                     /locus_tag="VF517_15785"
     CDS             1060..1527
                     /locus_tag="VF517_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054448.1"
                     /translation="MPLRPGTLPDEAAARRTLREQIARLESELGALFCSTFPRTGFDW
                     DVPSQGGGPRFLSLSELEQVRDRLADRLAHNRGLLGDRTYVEELNRRRIEEMLLNPAE
                     HKWVRVQNEDIGERGCKQWHVRPRFGILGMLMNWWRVRISSGCPLAMPATAGP"
     gene            1533..2084
                     /locus_tag="VF517_15790"
     CDS             1533..2084
                     /locus_tag="VF517_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054449.1"
                     /translation="MGKRSRKRGASGTDVSPSGTSRAERDEARRRRAQAAERARAASP
                     GATGRPRRRGWPTIDERPPAPWGNLPLVEIAVLLAIVLFVAGLVIGGERGQILLTGAL
                     GLGSLAGLELSIREHFAGYRSHTTLIAAVFAFVAMAVTFFAAGKGDAARALILPVAGI
                     TFMATFYWLREVFKRRSGGLGFR"
     gene            2081..2461
                     /locus_tag="VF517_15795"
     CDS             2081..2461
                     /locus_tag="VF517_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054450.1"
                     /translation="MIRNVVRSGESAVLMLTIMFVGSLGLWVGIPVGALFVGSRVQEA
                     SDSIALAMVAMTLMVIGSLVVLVPILGWLNHKYMEIRVARGRQDLGNAPLEGVIVVSA
                     AVAVVAFVIWVAFFAGTAPFTGSQ"
     gene            2458..2928
                     /locus_tag="VF517_15800"
     CDS             2458..2928
                     /locus_tag="VF517_15800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8054451.1"
                     /translation="MSLETTNPGGTPAPEPRRLAIRGIHHITLICANAERSAAFYRDL
                     LGLRLLESGVNQDDPGARHFYFGDAAGSPGTVITVMEYAQMDEGDVGRGSTHHFALTV
                     DSAEELEGWRQYLTSRGVGCTHVMDRQFFRSIYLRDPDGHIVELATRGPGFPEG"
     gene            complement(3002..3364)
                     /locus_tag="VF517_15805"
     CDS             complement(3002..3364)
                     /locus_tag="VF517_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054452.1"
                     /translation="MGETATTTAAPQHLRALHHANRVRLARAELKRKVASQQMSAAEV
                     VMACPWEAASMEVSDLLMSQRRWGRARCRRLLLSLGLPENKQIGTLTARQRQALAAVL
                     SAKAGANRILEAEALSTA"
     gene            3651..5063
                     /gene="pyk"
                     /locus_tag="VF517_15810"
     CDS             3651..5063
                     /gene="pyk"
                     /locus_tag="VF517_15810"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX8054453.1"
                     /translation="MRSTKIVATIGPASRDAPVLEAMIEAGLDVARLNFAHGDPEHHA
                     ETVGMVRAAAERVGRQVAVLQDVPGPKLRIGPVADEVANLEVGSSVVLTSERIEGDST
                     RLPVAWPGFAELLGTGDIVYLADGAIRLRVQDSRDGEVDVKVEVGGTVGSRQGMNLPN
                     VTMSLPAVSEDDIKLVDAGVRMGVDLMALSFIKTADDLRPVRERLATHDSDIPVLAKI
                     EKPQAAENVEEIVAAADGIMVARGDLGIELPIEEVPLVQKRLLYVAGQLAKPSITATQ
                     MLESMVQSTRPTRAEVTDVANAIFDGTDAVMLSQETAIGRDPAGVVRMMAAIAEATER
                     ELPYWRMLTERGLRGGDVDASVVFGAVGAVYQLGLKALVCPTMTGRTARLIASHRPQV
                     PTLALSPRPEIVRRCCLYWGVQGALHEEPEDTIGLLDTCVEAARNAGLATSGDRIGIT
                     AGVPMGVEGGSNLFNVHTLE"
     gene            complement(5082..6299)
                     /locus_tag="VF517_15815"
     CDS             complement(5082..6299)
                     /locus_tag="VF517_15815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8054454.1"
                     /translation="MAETVTDLFEKARNHERLEILRAAREHDLMPYFRLLEGEAGPVV
                     EMEGAERVMLGSNNYLGLNRDPRVKQAAADALERYGTALTGSRFLNGTIPLHLELERE
                     LAEWMGDEAALVFTTGYQANVGAIATLLSPTDTVICDSGDHASIMDAVAMSHAKVRPF
                     RHNRLDKLETMLSRATGDGGGVLVVVDGVFSMEGDVCDVPRVAELCRAHGARLMVDEA
                     HGVGVMGARGAGACEALGVEDSVDLRMGTFSKSLASCGGFISGPADVIDFLRVQSRAF
                     MFTAAAVPAAVGAALGALRIIRSPEGPDLLARLRENALYLENGLQELGFTVIESGGEH
                     VTPVVPVVIGDDWQAALMWKALFDAGVYVNVALYPAVERGAALLRTSVMATHEREHLD
                     RALEAFDRCRPAE"
     gene            6319..7476
                     /locus_tag="VF517_15820"
     CDS             6319..7476
                     /locus_tag="VF517_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054455.1"
                     /translation="MSLEVRPVRSRADLMRFIRLPWRIYSNEPLWVPPLVFERKRFLN
                     PRKNPWFEHAEAELFIAWRGRRPVGRISAQVDHDFNDYHGNNWGMFGFFECEDDPEAA
                     RALLAAAEDWLRGRGRDRMVGPMDFSMNDEAGLLIEGYDMAPMVKQPYHPRYYKPLLE
                     GAGLAKAIDLYMWTLNVTDKENMLPVMFDLAEKLEPEHGITIRHVKVGDLIKREMERF
                     VEVYNEAWKDNWGFVPLRVDEMVHAAKESRPVLHSDWLMVAEDRSGEIVAFALTALDV
                     NQVLAPMNGRLLPLGWLRFLWGVRRIDWVRVGFLGVRPELQHTGVAAGLYVEHFETAE
                     RHKYLKGGETGWILETNTAMNRGMEAMNGRIVKKYRVYERELATPSRNGSI"
     gene            7576..7863
                     /locus_tag="VF517_15825"
     CDS             7576..7863
                     /locus_tag="VF517_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054456.1"
                     /translation="MEDAAYTPPEVVDEDGRPLPVPVRSRAVAERPDGVLAAPMAATA
                     GGIVAGLATMMIVRGLRHRPKRVRVGGRRRGKALEVTATRSFLVDVHMLKR"
     gene            7863..8921
                     /locus_tag="VF517_15830"
     CDS             7863..8921
                     /locus_tag="VF517_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054457.1"
                     /translation="MATAAVRAAEPAAAPRGGEAAPPRRGAAAVRAAQPAAAPHRGPS
                     VVELDVIPPGPYRLPPPGRDGVMRRRDGALVRVLHCPEDREARVCAWIANGAVRLRAE
                     APTRECALYAVDRMRFALGLDHDIRPFLRRFRWDPLIGPIVRRRPWIRPLRRPEPFEA
                     LAWAITEQLIESERASAIQRVLVRRFGRRSDCGTLRDAPSAARLARCGQAELQACDLS
                     AGRSQALIRVSREVAAGRVDLSEHEPTWRRLRTIREIGPWTLECLALHGQGRDDQIPA
                     GDLAYVKLVGNLAGLGRRATVDEVYEFFEPYAPYAALAGQYMLNASYLKGPAAGTPTS
                     RSRSWPPRRGPSPRSSGR"
     gene            complement(8818..9486)
                     /locus_tag="VF517_15835"
     CDS             complement(8818..9486)
                     /locus_tag="VF517_15835"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX8054458.1"
                     /translation="MARRERRLSGRTALVVLFLVVGGLTLAGTGLLPRPEEFRENVES
                     WGALGAVAVFGIAVAHAVVSFPSEILNIAAGYAYGFWAALVLMMAAWTASCLVAYWLA
                     RSFGDRIANRFVEPAALDRLRDWAADAPVPVLLAVRAIPIMPINLLSYAAGLARMPLW
                     RFTWTTVAGLTPQFGLPIYAGSQARDVSITDPTIWGSGLAWVAMIAIGWWAYRRLAPS
                     DTTR"
     gene            complement(9496..10047)
                     /locus_tag="VF517_15840"
     CDS             complement(9496..10047)
                     /locus_tag="VF517_15840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX8054459.1"
                     /translation="MRILAISGSLRIGSHNTALVRTARDLAPAGVEIELFDGLDSIPA
                     FNEDRESVLPEPVQELNRRIREANALLVSTPEYNGGMPGALKNALDWASRPHGEAALV
                     AKPAAVISASTTAYGAKWAQEQVRRALTLSGAMVIDGELAVDRANDKVVGREVVDSAT
                     RAALASVVEDLAEAASAFESMAA"
     gene            complement(10052..10615)
                     /locus_tag="VF517_15845"
     CDS             complement(10052..10615)
                     /locus_tag="VF517_15845"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX8054460.1"
                     /translation="MSTATQPQAGAVEIPAGTWTVDPVHSTIGFEVKHFGISTFRGRF
                     TGYEGTLETGDGTLERVEGKVDVTSVDVRDEQLASHLQSEDFFDSANHPQIAFASTGV
                     RQVEDGYELDGDLTIRGVTKPVTLAVEVDGGGEDPFGGYRLSLVGRGEIDRFDYEIPF
                     NAKSPKGVLIAGEKVRLVLSVEAVKEA"
     gene            10714..11202
                     /locus_tag="VF517_15850"
     CDS             10714..11202
                     /locus_tag="VF517_15850"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8054461.1"
                     /translation="MANSVSQPKLTALHSLRRARHVLLDRLENALRADDLPPLAWYDV
                     LYALEGAGEHGLRPRDLAFEVALTPSGLTRLLDRIAAAGLVERSVCPSDRRGFNTTLT
                     PAGRDTLALMRPVYMRELEAGFGSLITDAEAEQISAILQRVSSSACTLAGEEAAPQAS
                     AA"
     gene            11250..12605
                     /locus_tag="VF517_15855"
     CDS             11250..12605
                     /locus_tag="VF517_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054462.1"
                     /translation="MRKALPLILLALTGLAPSAASAAEEELTLYSPPVRTLPYVHDTH
                     QLQLPADGIHAPKKPGYVVGVKEQVLVDSKDPDAKPLGNAQMMIHHLVYFAPGRAEDS
                     EGSCWRGAGFITARGEEHPEGNFDFMPADSRDRYGIVNKRPDGSAPDWHLVAMIMNHV
                     KKPKTVYVRTKIRYTEEERQPMYPTIVGCPVASGMAYDVPGGGQKGSQFVDQSTWTVP
                     YSARILLGTSHQHGGGIHQTLTSDTCNRRIFKANVYHGTPDHIYNTIRPILHEPGPVA
                     TGTFSSKAGIPVRRGEVLRRRAVHDNSNLHVASMGFWVLMLAKDEGVGDCDPMPTDVR
                     ELRRPKRFDKTPNFGLKVPQLDKPKGVFEPFTGGPVAVADDYFRPGLLDAKVGEQLTW
                     EFKGSKPHTVTVANGPRGFSSVYSGVRVGRFSFTPTVPGTYRLTCLVHPTTMGQTLRV
                     R"
     gene            complement(12610..13611)
                     /locus_tag="VF517_15860"
     CDS             complement(12610..13611)
                     /locus_tag="VF517_15860"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8054463.1"
                     /translation="MRKILSSMACAVLVAGAAAGCGEREEGGKPGGTEKLRLMLDFFP
                     NADHTPIYAAEAGGHFKNVGLDVEVRAPSDPAAPLRQVAAGRADIAVSYTPEVMRARD
                     KGAKVVAVGALVQRPLTSIISLPGARIAGPKDLRGKEIGTAGIDYQSAFLQAIVEKAG
                     VDPADVKERNVGFDLSPALLTKKVDATLGGFWNYEGTDLRLKKRDPRIIRVDQAGVPP
                     YDELVFVASEEAVEEKGDAIRGFLGAVARGTNDLRRDPRRGLDALLRANRDLDPRLQS
                     EVLKVTLPLFAPPKGRPFGWQEPREWQAFGKFMRDAGLIGRGADGAFTNDLLPGSGL"
     gene            complement(13608..14363)
                     /locus_tag="VF517_15865"
     CDS             complement(13608..14363)
                     /locus_tag="VF517_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8054464.1"
                     /translation="MLRRYAPTALLLAAAVAVWQGVASLGSVDDLTLASPVETVRAFG
                     DDASLLADNALITLAEVLLGLAIAVALGAGAAVAMHLQRPVREAAYPLLIASQAIPIV
                     VVAPLLILAFDYGIGPKIAIVALICFFPVTVNLLDGLRSVEPDLVKLMRSLGASRAAT
                     LWKVELPSSLPFLFSGLKIAATVSVIGAVFGEWAGADEGLGRMVLLGINQLETPRVYA
                     GVVLLTVIALALFVLVSLAERAAVPWTRREVRS"
     gene            complement(14357..15133)
                     /locus_tag="VF517_15870"
     CDS             complement(14357..15133)
                     /locus_tag="VF517_15870"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8054465.1"
                     /translation="MPAEGAMTTDLGAPSRLRTEEVLALRGVARRFGAVTALESLDLS
                     AHEGEILALVGPSGCGKSTLLELVAGLQRPDEGTVAVAGSAAYMPQRDLLLPWRDALG
                     NAALAPESAGVPRGEARRRAAPLFERLGLGQFENARPHELSGGMRQRVAFVRTLLSGR
                     PILLLDEPFGALDAITRTSMQEWLASALAAEPRTVLLVTHDVTEAILLADRVAVLSPR
                     PGRIVTTLCVDLPRPRVRRELVGDPAFAEFERRALEALGC"
     regulatory      complement(15101..15216)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15225..16730)
                     /locus_tag="VF517_15875"
     CDS             complement(15225..16730)
                     /locus_tag="VF517_15875"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8054466.1"
                     /translation="MRGAGLGTGAQPRAPGSVAASVGAAGGATPKRGGRALYGGLPEA
                     GPPGFAPRVLELADELRKEGLAVGTSELLDAFQALAELSWTAQEDFREALAATLAKSQ
                     EDRRVFELVFDRFFFRAVEREAVERGLTEQRYEGGERLDFDQLREAIRQAVRAGSDGE
                     MRDMARLAIAAFGRQGEGSGVIGVDVQRIRRALDLKGEVGARDPDQEPAPDTVPQERL
                     REFERHLRRELERALIERTQSLPPSKPLREFDRALPSNPSQDLASVHRIVAQLKRRLA
                     SQGNDPRGRRRSAVVDVRRTMRASLETGGVPLRLRYRSKRPKRPELYVLCDVSTSVTS
                     ASVFFLSVLHALHDSFRRLRTFVFVERVSEVTEVFESERSFKAISHAIATDAGVADVS
                     GYTDYGRVWIEFLADIVDDLDPRSTVIVLGDARTNGREPQAKAFEALSARAGRMFWLN
                     PEPKLYWNYGDSVMQVYEPYCDGVFECWSTKQLEAFVNALADTRARPAAAR"
     gene            complement(16759..17658)
                     /locus_tag="VF517_15880"
     CDS             complement(16759..17658)
                     /locus_tag="VF517_15880"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8054467.1"
                     /translation="MGDPGQSVDRMRDGLGEVGYLASESAALVAFLAVKLGKPVLVEG
                     PAGVGKTELAKALARSTERKLIRLQCYEGLDEAKALYEWNYRKQLLRIQTEAADTGWR
                     EVKDDIFGPDFLLTRPLLQAIAEDDPVVLLIDEIDKTDQEFEAMLLEVLSDFQISIPE
                     LGVVEAKSRPLVLLTSNNSRELTEALKRRCLYLWLDYPELERELEIVRLHAPELDETV
                     ARKLVEIVHMVRNLDLKKPPSIAESIDWARALIMLGADDIDSAVFERTLSVIVKHRTD
                     LDVVAERVGVKLDGGVLGRAAGQ"
     gene            17684..17920
                     /locus_tag="VF517_15885"
     CDS             17684..17920
                     /locus_tag="VF517_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054468.1"
                     /translation="MLAALLALLADFDLPDRPNIDPDKRFGTDPTPYLILFGVGFLTA
                     VLGHIVKVKVMVAAGILMVFLATVILPLVARSGY"
     gene            complement(17819..18628)
                     /locus_tag="VF517_15890"
     CDS             complement(17819..18628)
                     /locus_tag="VF517_15890"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8054469.1"
                     /translation="MRVGGSWASKGAAPPERAESRAAGRGTPLVRAGDERHRTHSILR
                     SVERSSDSRSGSRASRLARRAQAGDDLAFAELYVEFFDRVHRYLLIALKSPDDAQEVA
                     QEVFARALRRLDRFDHRRGEFRDWLFSMVRSVAIDHLRQGARAEEVEFRSIHAHALPV
                     ADRAASLLDRLDPESGVRSLIDGLPELQRRVLTLRFVFGFSTAEIGDVVGSTPDAVRH
                     IQHRALKTLGSGMESVAQLVTRAGHERQDHRREEDHQDPRGDHHLDLDDVA"
     gene            18922..>22254
                     /locus_tag="VF517_15895"
     CDS             18922..>22254
                     /locus_tag="VF517_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054470.1"
                     /translation="MRAELRRLVLAAALALAIFAAALGTATGETSDRESRSSRASIEG
                     SEARDAWAEARDSEQRRRAHRDSADGREERSRSRTAFRNQPDDQALAIARKKFPRLVD
                     APALEWPDLGPGRKLERYLSSNRALVDEPGGRKSVIESTLPLIGESPSGDKAPIDLAL
                     EDRGSSFAPKSSGAAIRIPKGARGALRFLDQDFGIALDGARAGDAKLSSNKAFYANAL
                     PDADLVIEPVPVGAEISLVLRSPDSPTKPALAFDLPPDARLRLVAHADSDSVAPGSAE
                     VVRDGKRVALVPPAQALDSQGESVPVGYEVDGDRLVMRVDTSGEIAWPVAVDPTTYIY
                     DNNGQSTCPPGGPLPCDGPPTGFNWPGWTPWSSRAQTNPCPYPSSQWFYECKSSDGAL
                     YVWGFTGFNYAANDRALWQKTARPGSYIFRFDAANMSHTASASQRFSGICGGPGCTGW
                     AASGQWFSNDPNSWANTPYRYYGASEMSRTEYYCVNSVHGQACTPQQAATDSSLLNNS
                     VIFGMIMNGGYASSQPHVAMGGAATYSSDNVAPNLTVVAHDPELPAGWVEGYTGTVRL
                     RAVDGAGANNGLGMGTLALTGGIGQNPLSTCTTNNAYDTCPTTWEPTAGLPYTTPPTD
                     GTYSYTASATDIVGNRNTAQDKGWSIKVDRTPPGAYATERARWVKDGVHQFTATSSDS
                     LSGVRQSRIWVARASDRFERTASNGWGTADQGGAWTVDEGPASDFSASPSTGGKIDFP
                     SGGGFRSASLYGRQVRNADVSARIKLGQPTSGTGVGSFANLMLRRQPNGGDNYRVGLI
                     GTNDGRLLVRGARNAGTFIDQDTGLGFTPGDSYRLRVVALSDAAGTATTVYVRYWRDG
                     DPEPATWNITTAPDSAGPQGAGAVGVRVNGGSTTAQQFYFDEIKLVDRDTTQTKDATK
                     ADGTPCDSVSGCPQSLAHTYQLNTAGYSEGPIEIVGSPQDPLAGAPNTSYDRHQRWDD
                     WQMKLDRTGPTFDAAPSPNPPYANLRPGENYEFTVDSRDNLSGVEKIELYVNGVLTGP
                     SVPGDNCSSAGCDTNPSARGMSYQIPADYSGSNVTFKLIATDNAGNESAPHVWRVNTD
                     DDPPTAPVISGALRAAQGKAVKG"
BASE COUNT         3256 a         8109 c         7838 g         3051 t
ORIGIN      
        1 ccaacgccac gcgcgggtgc gaggaccagc tcggccgggt cgagcaggtc cgccgcgagt
       61 tccccggggt ggccttcgtg ggggtcgtca gcaagcgcag ccgcgctgag gcggaggccc
      121 tcgcgcgcgc aaacggctgg ggcttcccgg tcgtggtgga ccgcgacgcg cagctcttca
      181 acctctacgg gatcggcgac tgcccgacga cgatcttcgc ccgccggggt ggcatcgcca
      241 accgctccgt gcgcggcgag ctcggcgcgg cgcggctgcg cgccgagctc gccgagatcc
      301 gatgacggag gagccgcggc tcgaacccgg ctggatcgcc gaggagctgc ggatcgagtt
      361 cccggagctg cgcctcgtca cgaccacgat cgacggcggg ccggcccgca gcccgaacgc
      421 ggtgaaggac cggctgcgcg tgctgagcaa ccgctacacg ggccagaagg cgatcgccct
      481 gcgccagcag ccgatcccgt gggcctaccg cgtgttcttc cgccaggtgg ggatcgaccc
      541 cgacgaccac cgcacgcccg tggaggccta cgcgctcgaa cgcatgcgcg cgggcggctt
      601 ccagagccag aacctgctgc tcgacgcgct gacgatcgcg accgtcgaga ccggcgtgcc
      661 ggtggtcgcg ttcgacgaga cgaccctcga cgggcccctc ggcctgcgcc tgtcggagcc
      721 aggggagctg ctcgggggag acggccgccc gctgtcgtcg cgccaggtgg tgatcgcgga
      781 cgcggagcgc tccgtggcag tcctgttcgc ggacttcgcg gagggagtcg agccgcgctc
      841 gcggacccgc cgcctgcggc tcgcctccgt gcaggtgaag ggcgtgccgg acgtgagcgt
      901 cgaggaggct ctgtggacgg tcgccgagac cgtgacgggg accgagctgc cgtagcccgg
      961 gggccgatcc cggtgctacg atcgcgacag cgacggcgag gaggaccgat ggccgaagta
     1021 ttcgaactgc gcgcgggagt accggccccc gtctctccga tgccgcttcg cccggggacg
     1081 ctccccgacg aggcggccgc gcggcgcacg ctgcgcgagc agatcgcccg gctcgagtcc
     1141 gagctcggcg cgctcttctg ctccaccttc ccgcgcacgg gcttcgactg ggacgtcccg
     1201 tcgcagggcg gcggcccgcg cttcctctcg ctgagcgagc tggagcaggt gcgcgatcgc
     1261 ctcgccgacc ggctcgcgca caaccgcggc ctgctcggcg accgcaccta tgtcgaggag
     1321 ctgaaccgcc gccgcatcga ggagatgctg ctcaaccccg ccgagcacaa gtgggtgcgc
     1381 gtccagaacg aggacatcgg cgagcgcggc tgcaagcagt ggcacgtccg cccgcgcttc
     1441 ggcatcctcg ggatgctgat gaactggtgg cgggtccgca tctcctcggg ctgcccgtta
     1501 gccatgccgg cgacggccgg tccctgatcc aggtgggtaa gcgaagccgc aagcgcgggg
     1561 cctccggcac cgacgtctcg ccctcgggca cgagccgtgc cgagcgcgac gaggcgcgcc
     1621 gccgccgcgc gcaggccgcc gagcgcgcgc gggcggctag cccgggcgcg acgggccgcc
     1681 cgcgtcgccg cggctggccc accatcgacg agcgcccgcc ggctccgtgg ggaaacctcc
     1741 cgctggtcga gatagcggtg ctgctcgcga tcgtcctgtt cgtggctggg ctcgtgatcg
     1801 gcggcgagcg cggtcagatc ctgctgaccg gcgcgctcgg cctggggtcg ctggccgggc
     1861 tcgagctctc gatccgcgag cacttcgccg ggtaccgctc gcacaccaca ctgatcgccg
     1921 cggtcttcgc gttcgtggcg atggcggtca ccttcttcgc cgccggcaag ggcgacgccg
     1981 cgcgcgcgct gatcctcccg gtcgcgggca tcaccttcat ggcgaccttc tactggctgc
     2041 gcgaggtgtt caagcgccgg tccggagggc tcgggttccg gtgatccgca acgtcgtacg
     2101 cagcggcgag agcgccgtgc tcatgctgac gatcatgttc gtcggcagcc tcgggctgtg
     2161 ggtcggcatc ccggtcgggg cgctcttcgt cggctcgcgc gtgcaggagg cgagcgactc
     2221 gatagcgctc gcgatggtcg ccatgacgct gatggtgata ggaagccttg tcgtgctcgt
     2281 cccgatcctc ggctggctga accacaaata catggagatc cgcgtcgccc gcgggcgcca
     2341 ggacctgggc aatgcgccgc tcgagggcgt catcgtggtg agcgcggcgg tggccgtcgt
     2401 ggccttcgtg atctgggtgg ccttcttcgc cggcacggcg ccgttcacgg ggtcgcagtg
     2461 agcctcgaga ccaccaaccc cggcggcacg cccgcccccg agccgcgccg gctcgccatt
     2521 cgcggcatcc accacatcac gctgatctgc gccaacgcgg agcgctcggc ggcgttctac
     2581 cgcgacctgc tcgggctgcg gctgctcgag tcgggggtca accaggacga ccccggcgcg
     2641 cggcacttct acttcggcga cgcggccggc agccccggca cggtcatcac ggtgatggag
     2701 tacgcgcaga tggacgaggg cgatgttggc cgcggctcga cccaccactt cgccctgacc
     2761 gtggattccg ccgaggagct cgagggctgg cgccagtacc tcacctcgcg cggagtcggc
     2821 tgcacgcatg tcatggaccg ccagttcttc aggtcgatct acctgcgcga ccccgacggg
     2881 cacatcgtcg agctggcgac ccgcgggccc ggttttcccg aaggctgagg cgcaggcgcc
     2941 ggctcgggcg gccgggcggg ggcggcgagg ggaccgccaa cccgcccggc gcgcaccggt
     3001 tctaggccgt cgagagcgcc tccgcttcca ggatgcggtt ggcgcccgcc ttggccgaga
     3061 ggaccgccgc gagggcctgt cgctgcctcg cggtgagagt tccgatctgc ttgttctcgg
     3121 ggagcccgag cgagaggagc agccgccggc agcgcgcgcg tccccagcgc cgctggctca
     3181 tcaggaggtc gctcacctcc atgctcgccg cctcccacgg gcaggccatc acgacctctg
     3241 cggcagacat ctgctgcgac gccaccttcc gcttcagctc cgcgcgcgcg agccgcacgc
     3301 ggttggcgtg atggagtgcc cgcagatgtt gtggcgcagc agtggtcgtg gcggtctcgc
     3361 ccattcgtcc tccccagaag acagttgttc gcgcttgccg atcgcagcaa ccatccagaa
     3421 cgcggttggt gtcccgaacg acaccccctc gcacggcctc ccttggtccg tctgcccgtc
     3481 tggtcgctgc ggcggatgaa acctaagccg agtgatcccc ggtggtcaac cggccaaacg
     3541 aagctctgcc gctaagagcg ggcttttcct cagaagggct cgcgcaacgc ctaccccatg
     3601 cggcggacag ctcaacttga ggtcccccgt gaggtgtata cctcccctcg atgcggtcaa
     3661 cgaaaatcgt cgccacgatc ggtccggcgt cgcgtgatgc gccggtgctc gaggcgatga
     3721 tcgaggccgg gctcgacgtg gcgcggctga acttcgcgca cggggacccg gagcaccacg
     3781 cggagaccgt cggcatggtc cgggccgccg ccgagcgggt cgggcgccag gtggcggtcc
     3841 tccaggacgt gcccgggccg aagctgcgga tcgggccggt cgcggacgag gtggccaatc
     3901 tcgaggtggg cagcagcgtg gtgctcacga gcgagcggat cgagggcgac tcgacgcgcc
     3961 tgccggtggc ctggcccggg ttcgccgagc tgctcggcac cggcgacatc gtctacctcg
     4021 ccgacggtgc gatccggctg cgcgtgcagg actcgcgcga cggcgaggtc gacgtgaagg
     4081 tcgaggtggg ggggaccgtc ggctcgcgcc aggggatgaa cctgcccaac gtgacgatgt
     4141 cgctcccggc ggtgagcgag gacgacatca agctggtcga cgccggcgtg cggatgggcg
     4201 tcgacctcat ggcgctgtcg ttcatcaaga ccgccgacga cctgcgtccc gttcgcgagc
     4261 gcctggccac ccacgacagc gacatcccgg tgctggccaa gatcgagaag ccgcaggccg
     4321 cggagaacgt ggaggagatc gtcgcggccg ccgacgggat catggtcgcg cgcggcgacc
     4381 tcgggataga gctgccgatc gaggaggtgc cgctcgtcca gaagcgcctg ctctacgtcg
     4441 ccggccagct cgccaagccc tcgatcacgg ccactcagat gctcgagtcg atggttcagt
     4501 ccacgcgacc gacccgtgcg gaggtgaccg acgtcgccaa cgcgatcttc gacggcaccg
     4561 acgcggtgat gctctcccag gagaccgcga tcgggcgcga ccccgcgggc gtggtgcgga
     4621 tgatggccgc gatcgcggag gcgaccgagc gcgagctgcc gtactggcgg atgctcaccg
     4681 agcgcggcct gcgcggcggc gacgtcgacg cgtcggtggt gtttggcgcc gtcggtgcgg
     4741 tctaccagct cggcctcaag gcgctcgtgt gccccacgat gaccgggcgc accgcgcggc
     4801 tgatcgcctc gcaccgcccg caggtgccca cgctcgcgct ctcgccgcga ccggagatcg
     4861 tgcgccgctg ctgcctctac tggggcgtgc agggggcgct gcacgaggag cccgaggaca
     4921 cgatcggcct gctcgatacg tgtgtcgagg ctgcgcgcaa cgccgggctc gccacgtcgg
     4981 gcgaccggat cgggatcacg gccggcgtgc cgatgggcgt cgagggcggc tcgaacctct
     5041 tcaacgtcca caccctcgag tagcgcgccg gcgcgcggcg gctactcggc gggccggcag
     5101 cggtcgaacg cttcgagcgc ccggtccagg tgctcgcgct cgtgcgtggc catcacgctg
     5161 gtccgcagca gcgccgcgcc gcgctcgacc gcggggtaga gcgccacgtt cacgtacacg
     5221 cccgcgtcga agagcgcctt ccacatcagc gccgcctgcc agtcgtcgcc gatcaccact
     5281 ggcacgaccg gggtcacgtg ttccccgccg ctctcgatca cggtaaaacc gagctcctgg
     5341 aggccgttct cgaggtagag ggcgttctcg cgcaggcgcg ccagcaggtc cgggccctcc
     5401 ggcgagcgga tgatcctgag cgcgccgagc gccgcgccga ccgcggccgg gaccgccgcg
     5461 gcggtgaaca tgaacgcgcg cgactggacg cgcaggaagt cgatcacgtc ggctgggccg
     5521 gagatgaagc cgccgcagct cgcgagcgac ttcgagaagg tgcccatgcg caggtccacg
     5581 gagtcctcga ccccgagcgc ctcgcacgcc ccggccccgc gggcgcccat gacgccgacg
     5641 ccgtgggcct cgtcgaccat cagccgcgcg ccgtgggcgc ggcagagctc ggccacccgc
     5701 ggcacgtcgc agacgtcgcc ctccatcgag aagacgccgt cgaccaccac gagcacgccg
     5761 ccgccgtcgc ctgtggcgcg ggagagcatc gtctcgagct tgtcgagccg gttgtggcgg
     5821 aacggccgca ccttcgcgtg cgacatcgcg accgcgtcca tgatcgaggc gtggtcgccg
     5881 gagtcgcaga tcacggtgtc ggtgggggag aggagcgtgg cgatcgctcc cacgttcgcc
     5941 tggtagccgg tggtgaagac gagcgcggcc tcgtcgccca tccactccgc cagctcgcgc
     6001 tcgagctcga ggtggagcgg gatcgtgccg ttcaggaagc gcgagccggt gagcgcggtg
     6061 ccgtagcgct cgagcgcgtc cgccgcggcc tgcttcacgc gcggatcgcg gttgagcccg
     6121 aggtagttgt tcgacccgag catcacgcgc tcggcgccct ccatctccac gaccggcccg
     6181 gcctcgccct cgagcagccg gaagtacggc atgaggtcgt gctcccgcgc ggcgcgcagg
     6241 atctcgaggc gctcgtggtt gcgcgccttc tcgaacagat cggtgacggt ctcggccatt
     6301 cgagtagggt caggaagcgt gtcgctcgag gtacgcccgg tccgctcgcg tgcggacctg
     6361 atgcgcttca tacgcctgcc gtggcgcatt tactctaacg agccgctctg ggtccccccg
     6421 ctcgtgttcg agcgcaagcg gttcctgaat ccgcgcaaga acccgtggtt cgagcacgcc
     6481 gaggccgagc tgttcatcgc ctggcgcggg cggcgcccgg tgggccggat cagcgcccag
     6541 gtcgaccacg acttcaacga ctaccacggc aacaactggg ggatgttcgg gttcttcgag
     6601 tgcgaggacg atcccgaggc ggcacgcgcg ctgctcgccg ccgcggagga ctggctgcgt
     6661 gggcgcggcc gcgaccggat ggtcggcccg atggacttct cgatgaacga cgaggccggc
     6721 ctgctgatcg agggctacga catggccccg atggtcaagc agccctacca cccgcgctac
     6781 tacaagccgc tgctcgaggg cgcggggctg gcgaaggcga tcgacctcta catgtggacg
     6841 ctgaacgtca ccgacaagga gaacatgctc ccggtgatgt tcgatctggc cgagaagctc
     6901 gagccggagc acggcatcac gatccgccac gtgaaggtgg gcgacctgat caagcgcgag
     6961 atggagcgct tcgtcgaggt ctacaacgag gcctggaagg acaactgggg gttcgtgccg
     7021 ctgcgggtgg acgagatggt ccacgcggcg aaggagtcgc ggccggtgct gcactcggac
     7081 tggctgatgg tcgccgagga ccgctccggc gagatcgtcg ctttcgcgct caccgcgctc
     7141 gacgtcaacc aggtgctggc gccgatgaac gggcgcctgc tgccgctcgg ctggctgcgc
     7201 ttcctgtggg gggtgaggcg gatcgactgg gttcgggtcg gcttcctcgg cgtgcggccg
     7261 gagctccagc acaccggggt ggcggcgggg ctgtacgtgg agcacttcga gacggccgag
     7321 cgccacaagt acctgaaggg cggcgagacc ggctggatcc tcgagaccaa caccgccatg
     7381 aaccggggca tggaggcgat gaacggccgg atcgtgaaga agtaccgggt gtacgagcgc
     7441 gagctcgcga ccccgtcccg gaacggcagc atctaggttt tccacgacgt tcctgactta
     7501 tgggtcgtat ggacccacaa gtcaggaatg tcggatgaaa cggggcgccg accgcggggg
     7561 tagactcgcc cgctcgtgga ggacgccgcc tacacgccgc ccgaggtcgt cgacgaggac
     7621 ggccggccgc tgccggtccc cgtccgcagc cgcgctgtgg ccgagcgccc ggacggggtg
     7681 ctcgccgccc cgatggccgc tacggccggc ggcatcgtgg ccgggctcgc gacgatgatg
     7741 atcgtccgcg ggctgcgcca ccgccccaag cgtgtccgcg taggcgggcg ccgccggggc
     7801 aaggcgctcg aggtcacggc cacgcggtcg ttcctcgtcg acgtgcacat gctcaagcgc
     7861 tagtggcgac cgccgccgtg cgcgcagccg agccggccgc cgctccgcgc gggggagagg
     7921 ccgcaccgcc gcgccgcggg gccgccgccg tgcgcgcagc ccagccggcc gccgctccgc
     7981 accgcggtcc ctccgtcgtc gagctcgacg tgatcccgcc cggcccgtac cggctccctc
     8041 cgcccgggcg cgacggcgtg atgcgccgcc gtgacggcgc gctcgtgcgg gtgctccact
     8101 gcccggagga ccgggaggcg cgggtctgcg cctggatcgc caacggggcc gtgcggctcc
     8161 gcgccgaggc gcccacgcgc gagtgcgcgc tctacgcggt cgaccggatg cggttcgccc
     8221 tcgggctcga ccacgacatc cggccgttcc tgcgccgctt ccgctgggat ccgctgatcg
     8281 gtccgatcgt gcgccggcgc ccgtggatcc gcccgctgcg ccggccggag ccgttcgagg
     8341 cgctggcgtg ggcgatcacc gagcagctca tcgagtccga gcgcgcttcg gcgatccagc
     8401 gcgtcctggt gcgccgcttc ggccgccgca gcgactgcgg cacgctgcgc gacgcgccgt
     8461 cggcggcacg gctcgcgcgc tgcggtcagg ccgagctcca ggcgtgcgac ctctcggccg
     8521 gccgctccca ggcgctgatc cgggtgagcc gcgaggtcgc ggcgggtcgc gtggacctga
     8581 gcgagcacga gcccacctgg cggcgcctgc gcaccatccg cgagatcggc ccgtggacgc
     8641 tcgagtgcct cgccctccac ggccagggcc gcgacgatca gatcccggcg ggcgacctcg
     8701 cgtacgtgaa gctggtcggc aacctggccg gcctcggccg gcgcgccacg gtcgacgagg
     8761 tctacgagtt cttcgagccc tacgcgccct acgccgcgct tgcgggccag tacatgctca
     8821 acgcgtcgta tctgaagggg ccagccgccg gtacgcccac cagccgatcg cgatcatggc
     8881 cacccaggcg aggcccgagc cccagatcgt cgggtcggtg atcgacacgt cgcgcgcctg
     8941 gctgccggcg tagatcggca gcccgaactg cggggtgagc cccgccaccg tcgtccaggt
     9001 gaagcgccag agcggcatcc gcgcgaggcc ggccgcgtac gagaggaggt tgatcggcat
     9061 gatcgggatc gcgcgcacgg cgagcagcac cggcaccggc gcgtccgccg cccagtcgcg
     9121 caggcggtcg agcgccgcgg gctccacgaa ccggttcgcg atccggtcgc cgaacgaccg
     9181 cgccagccag tacgcgacca gacaggacgc cgtccacgcg gccatcatca gcaccagcgc
     9241 cgcccagaac ccgtacgcgt agccggcggc gatgttgagg atctcggagg ggaacgagac
     9301 gacagcatga gccaccgcga tcccgaacac cgccaccgcg ccgagcgcgc cccacgactc
     9361 gacgttctcg cggaactcct ccggccgcgg cagcagcccg gtgccggcga gcgtgagccc
     9421 gccgacgacc aggaacagaa cgacgagcgc ggtccgtccg ctcaaccgcc gctcacgccg
     9481 ggccacggcg cgatcctacg cggccatcga ctcgaacgcg ctcgctgcct cggccaggtc
     9541 ctccaccacg ctcgccagcg ctgcgcgggt ggccgagtcc acgacctcgc ggcccacgac
     9601 cttgtcgttg gcgcgatcga ccgcgagctc gccgtcgatc accatcgcgc cggacagcgt
     9661 cagcgcgcgc cggacctgct cctgcgccca cttcgccccg taggcggtgg tgctcgcgct
     9721 gatcacggct gccggcttcg cgacgagggc cgcctcgccg tgcgggcgcg aggcccagtc
     9781 gagcgcgttc ttcagcgcgc ccggcatccc gccgttgtac tccggggtgg agacgagcag
     9841 ggcgttcgcc tcccggatcc ggcggttcag ctcctgcacc ggctccggca gcaccgactc
     9901 gcggtcctcg ttgaaggccg ggatggagtc gagcccgtcg aagagctcga tctccacccc
     9961 cgccggagcg agatcccgcg cggtacggac gagggccgtg ttgtgggagc cgatccgcag
    10021 gcttcccgat atcgcgagga tccgcatcgt cctacgcctc cttgactgcc tcgaccgaca
    10081 ggaccaggcg gaccttctcg ccggcgatga gcacgccctt gggcgacttc gcgttgaacg
    10141 ggatctcgta gtcgaaccgg tcgatctcgc cacgcccgac caggctcagg cggtacccgc
    10201 cgaacgggtc ctcaccgccg ccgtcgacct cgaccgccag cgtgaccggc ttggtgacgc
    10261 cgcggatggt caggtcgccg tcgagctcgt agccgtcctc gacctgccgc acgcccgtgc
    10321 tcgcgaacgc gatctgcggg tggttggcgg agtcgaagaa gtcctcgctc tggaggtggc
    10381 tcgccagctg ctcgtccctg acgtcgacgc tcgtgacgtc gaccttgccc tcgacccgct
    10441 cgagcgtccc gtcgccggtc tcgagcgtgc cctcgtagcc ggtgaaccgg ccgcggaacg
    10501 tcgagatgcc gaagtgcttg acctcgaacc cgatcgtgga gtggaccgga tcgaccgtcc
    10561 aggtgcccgc cgggatctcc accgctcccg cctgcggctg cgttgccgtg ctcattctcg
    10621 gtgcctcctt gttgattcag cagtagttga ctgcgcaagg aacagactag cagcgtttgt
    10681 tgacacgtca acctttgtgc atacactgaa ggcatggcca attccgtctc tcaacccaag
    10741 ctcacggcgc tccactcgct gcgccgtgcc cggcacgtgc tcctcgaccg gctcgagaac
    10801 gcgctgcgcg ccgacgacct gccaccgctc gcgtggtacg acgtgctcta cgcgctcgag
    10861 ggcgcgggag agcacggcct gcgcccgcgc gacctcgcgt tcgaggtggc gctcacgccg
    10921 agcggcctca cccgcctgct cgaccgcatc gccgcagccg ggctggtcga gcgcagcgtg
    10981 tgcccgagcg accgccgcgg gttcaacaca acgctgacgc ccgccggccg cgacacgctc
    11041 gcgctgatgc ggcccgtcta catgcgcgag ctcgaggccg gcttcggcag cctgatcacc
    11101 gacgccgagg ccgagcagat cagcgcgatc ctccagcgcg tgagcagcag cgcgtgcacg
    11161 ctcgccggcg aggaggccgc gccgcaggcc agcgcggcct agccaagcgg aggtcgagca
    11221 tctaggctgg cggtgccggc ggactcgcaa tgcgcaaggc cctacccctc atcctcctgg
    11281 ccctgaccgg gctcgcaccc tcggctgcct ccgcggcgga ggaggagctg acgctctact
    11341 cgccgccggt caggacgctg ccgtacgtgc acgacacgca ccagctccag ctcccggccg
    11401 acggcatcca cgcgccgaag aagccgggct acgtggtcgg cgtgaaggag caggtgctgg
    11461 tggacagcaa ggatcccgac gcgaagccgc tcggcaacgc gcagatgatg atccaccacc
    11521 tcgtctactt cgcgcccggc cgcgccgagg actccgaggg aagttgctgg cgcggcgccg
    11581 gcttcatcac cgcgcgcggt gaggagcacc ccgagggcaa cttcgacttc atgcccgccg
    11641 acagccgcga ccgctacggc atcgtcaaca agcgcccgga cggctcagcc cccgactggc
    11701 acctggtcgc gatgatcatg aaccacgtca agaagcccaa gacggtctac gtgcgcacga
    11761 agatccgcta caccgaggag gagcgccagc cgatgtaccc gacgatcgtc ggctgcccgg
    11821 tcgcgagcgg catggcgtac gacgtcccgg gcggggggca gaagggctcg cagttcgtcg
    11881 accagtccac ctggacggtg ccgtacagcg cccggatcct gctcggcacc tcgcaccagc
    11941 acggcggcgg catccaccag acgctcacga gcgacacctg caaccgccgc atcttcaagg
    12001 cgaacgtcta ccacggcacg cccgatcaca tctacaacac gatccgcccg atcctgcacg
    12061 agcccgggcc cgtcgcgacc ggcacgttca gctcgaaggc cggcatcccg gtccggcggg
    12121 gcgaggtgct gcggcgcagg gccgtgcacg acaactcgaa cctgcacgtg gcctcgatgg
    12181 gcttctgggt cctcatgctc gcgaaggacg agggcgtggg tgactgcgac ccgatgccga
    12241 ccgacgtgcg cgagctgcgc cgcccgaagc gattcgacaa gacgcccaac ttcggcctga
    12301 aggtgccgca gctcgacaag cccaagggcg tgttcgagcc gttcaccggc ggcccggtcg
    12361 cggtcgccga cgactacttc cgccccggat tgctcgatgc caaggtgggc gagcagctga
    12421 cctgggagtt caagggctcg aagccgcaca cggtcaccgt cgccaacggg ccgcgcgggt
    12481 tctcgtcggt ctacagcggc gtgagggtcg gccgcttcag cttcacgccg accgtgcccg
    12541 gcacctaccg cctgacctgt ctggtccatc cgaccacgat gggccagacg ctcagggttc
    12601 gctgaccgcc tagagcccgg agcccggcag caggtcgttc gtgaacgcgc cgtcggcgcc
    12661 gcggccgatc agtccggcgt cgcgcatgaa cttgccgaac gcctgccact cgcgcggctc
    12721 ctgccagccg aacgggcgcc ccttcggcgg cgcgaacagc ggaagcgtga ccttcaggac
    12781 ctcgctctgg agccgcgggt cgaggtcgcg gtttgcccgc aggagcgcgt cgaggcccct
    12841 gcgcgggtcg cgccgcaggt cgttcgtccc gcgggccacc gcgccgagga agccgcggat
    12901 cgcgtcgccc ttctcctcga cggcttcctc gctcgcgacg aagaccagct cgtcgtacgg
    12961 cggcacgccg gcctggtcca cgcggatgat ccgcggatcg cgcttcttca gccgcaggtc
    13021 ggtcccctcg tagttccaga agccgccgag cgtggcgtcg accttcttgg tgagcagggc
    13081 cgggctcagg tcgaacccca cgttgcgctc cttcacgtcg gcggggtcca cgcccgcctt
    13141 ctcgacgatc gcctggagga acgccgactg gtagtcgatc ccggcggtgc cgatctcctt
    13201 gccgcgcagg tccttcgggc ccgcgatcct cgcgcccggc agcgagatga tcgacgtgag
    13261 cgggcgctgc accagcgcgc cgacagccac gaccttcgcg cccttgtcgc gcgcgcgcat
    13321 gacctcgggc gtgtacgaga ccgcgatgtc ggcgcggccc gcggccacct ggcgcagcgg
    13381 cgccgctggg tcggacggcg cgcgcacctc gacgtcgagc ccgacgttct tgaaatgccc
    13441 gcccgcctcg gcggcgtaga tcggcgtgtg gtcggcgttc gggaagaagt cgagcatcag
    13501 ccgcagcttc tccgtgccgc ccggcttgcc gccctcctcg cgctcgccgc atcccgccgc
    13561 ggccccggcc acgagcaccg cgcaggccat cgacgacagg atcttcctca cgagcgcacc
    13621 tctcttcgag tccaggggac ggccgcgcgc tcggcgagcg acacgagtac gaacagcgcg
    13681 agcgcgatga cggtcagcag caccacgccc gcgtacacgc gcggggtctc gagctggttg
    13741 atcccgagca ggaccatccg gccgaggccc tcgtcggcgc cggcccactc tccgaagacg
    13801 gcgccgatga ccgagaccgt ggccgcgatc ttcaggccgc tgaacaggaa cgggagcgac
    13861 gacggcagct cgaccttcca gagcgttgcc gcgcgggagg cgccgaggct gcgcatcagc
    13921 ttgaccaggt cgggctcgac cgagcgcagc ccgtcgagca ggttcacggt caccgggaag
    13981 aagcagatca gcgcgacgat cgcgatcttc ggtccgatgc cgtagtcgaa cgcgaggatc
    14041 aggagcggcg cgaccaccac tatcgggatc gcctgcgagg cgatcagcag cgggtaggcc
    14101 gcctcgcgga ccgggcgctg gaggtgcatc gccaccgccg caccggcgcc cagcgccacc
    14161 gcgatcgcca gtccgagcag cacctcggcc agcgtgatga gcgcgttgtc ggccagcagc
    14221 gaggcgtcgt cgccgaaggc gcgcacggtc tcgaccggcg aggcgagcgt gaggtcgtcg
    14281 accgagccca ggctggccac gccctgccac acggcgacgg cggcggcgag caggagcgcg
    14341 gtgggggcgt agcgcctcag cacccgagcg cctccagcgc gcggcgctcg aactcggcga
    14401 aggcggggtc gccgaccagc tcgcggcgca cgcgcggccg cgggaggtcc acgcacagtg
    14461 tcgtgacgat ccggccgggg cgcggcgaga gcacggccac gcgatcggcc agcaggatcg
    14521 cctcggtcac gtcgtgggtc acgagcagga ccgtgcgcgg ctcggccgcg agcgcgctgg
    14581 cgagccactc ctgcatcgac gtgcgcgtga tggcgtccag cgcaccgaac ggctcgtcga
    14641 ggagcaggat cgggcgtccg gagagcaggg tgcgcacgaa ggccacacgc tggcgcatgc
    14701 cgcccgagag ctcgtggggg cgggcgttct cgaactgccc gaggccgagg cgctcgaaga
    14761 gcggagccgc ccggcgccgc gcctccccgc gcggcacgcc cgcggactcg ggcgcgaggg
    14821 cggcgttgcc gagcgcgtca cgccagggga gcaggaggtc gcgctggggc atgtaggcgg
    14881 cgctgccggc gaccgccacg gtcccctcgt cgggccgctg gagcccggcg acgagctcga
    14941 gcagggtgga cttgccgcag ccggacgggc cgacgagcgc gaggatctcg ccctcgtgcg
    15001 cggagaggtc gagcgactcg agcgcggtca cggcgccgaa ccttcgggcc accccccgca
    15061 gcgcgagaac ctcctcggtt cgcaggcggc tcggggcccc caggtctgtc gtcatcgctc
    15121 cctccgcggg cattacccca caggttcgaa gggtcagcgc cgggatccgt ggcgctatct
    15181 cagcccgccg tcccgcgagc ccccctggtt tccgtacggc gatgctaccg agcggcggcc
    15241 ggccgcgcgc gcgtatcggc cagcgcgttc acgaacgcct cgagctgctt ggtcgaccag
    15301 cactcgaaca cgccgtcgca gtacggctcg tagacctgca tcaccgagtc cccgtagttc
    15361 cagtagagct tcggctccgg gttcagccag aacatgcggc cggcgcgcgc ggacagcgcc
    15421 tcgaacgcct tggcctgggg ctcgcggccg ttcgtgcggg cgtcgccgag gacgatcacg
    15481 gtcgagcgcg gatcgaggtc gtccacgatg tcggcgagga actcgatcca gacccgcccg
    15541 tagtcggtgt acccggacac gtcggccacg ccggcgtcgg tggcgatggc gtgcgagatc
    15601 gccttgaacg agcgctcgct ctcgaacacc tcggtcacct cggacacgcg ctccacgaac
    15661 acgaacgtgc gcaaccgccg gaacgagtcg tgcagcgcgt ggagcacgct caggaagaac
    15721 acgctcgcgc tggtcacgct cgtggacacg tcgcagagca cgtacagctc cgggcgcttc
    15781 ggacgtttcg agcggtagcg cagccgcagc ggcacgcctc ccgtctcgag cgacgcgcgc
    15841 atggtgcgcc gcacgtccac cacggccgag cggcgccgcc cgcgcgggtc gttgccctgc
    15901 gaggccagcc ggcgcttgag ctgcgccacg atcctgtgca ccgacgccag gtcctgggac
    15961 gggttgctcg gcagcgcgcg gtcgaactcg cgcaggggct tcgagggcgg gagcgactgg
    16021 gtgcgctcga tcagcgcgcg ctcgagctcg cggcgcaggt ggcgctcgaa ctcgcgcagg
    16081 cgctcctgcg ggaccgtgtc cggcgccggc tcctggtcgg ggtcgcgcgc cccgacctcg
    16141 cccttgaggt cgagtgcccg cctgatccgc tgcacgtcca cgccgatcac gccggagccc
    16201 tcgccctggc ggccgaaggc ggcgatcgcg aggcgcgcca tgtcgcgcat ctcgccgtcg
    16261 gaccccgccc gcacggcctg gcggatcgcc tcacggagct ggtcgaagtc gagccgctcg
    16321 ccgccctcgt agcgctgctc ggtgagcccg cgctcgacgg cctcgcgctc gacggcgcgg
    16381 aagaagaagc ggtcgaagac gagctcgaac acgcgccggt cctcctgcga tttcgcgagg
    16441 gtcgcggcga gggcctcgcg gaagtcctcc tgcgccgtcc agctcagctc ggcgagcgcc
    16501 tggaaggcgt cgagcagctc gctcgtgccg acggcgaggc cctccttgcg cagctcgtcg
    16561 gcgagctcga gcacccgcgg ggcgaagccg ggcggccccg cctcgggaag cccgccgtag
    16621 agggcgcgcc cgccgcgctt cggcgtggcc ccgccggcgg cgccgacgct cgcagcaacg
    16681 ctgccagggg cgcgcggctg cgcgccggtt ccgagacccg ccccgcgcat cgcgcggcgg
    16741 gtcgcgtcct cgcccggcct actgcccggc ggcccgcccg aggacgccac cgtcgagctt
    16801 cacccccaca cgctcggcca cgacgtcgag gtcggtccgg tgcttcacga tcacgctgag
    16861 cgtccgctcg aagaccgcgc tgtcgatgtc gtcggcgccg agcatgatca gcgcgcgcgc
    16921 ccagtcgatc gactcagcga tcgagggcgg cttcttcaga tcgaggttgc gcaccatgtg
    16981 cacgatctcg acgagcttgc gcgccacggt ctcgtcgagc tccggcgcgt gcagccgcac
    17041 gatctcgagc tcgcgctcga gctccgggta gtcgagccac aggtagaggc agcggcgctt
    17101 gagggcctcg gtcagctcgc gcgagttgtt cgaggtcagc agcacgagcg gccggctctt
    17161 cgcctcgacc accccgagct ccgggatcga gatctggaag tccgacagga cctcgagcag
    17221 catcgcctcg aactcctggt cggtcttgtc gatctcgtcg atgagcagca cgacggggtc
    17281 gtcctcggcg atcgcctgga gcagcggccg cgtgagcagg aagtccggtc cgaagatgtc
    17341 gtccttgacc tcgcgccagc cggtgtcggc ggcctcggtc tggatccgca agagctgctt
    17401 gcggtagttc cactcgtaca gggccttggc ctcgtcgagc ccctcgtagc actgaagcct
    17461 gatcagcttg cgctcggtcg agcgcgcgag cgccttcgcc agctcggtct tgcccacgcc
    17521 ggccgggccc tccacgagca ccggcttgcc gagcttgacc gccaggaacg ccacgagcgc
    17581 cgccgactcg gaggcgagat agccgacctc gcccagcccg tcgcgcatcc gatcgacgct
    17641 ctgccccggg tcgcccaccc cgggaaggat actttcggtc cgaatgctcg ccgccctact
    17701 ggccctgctc gcggacttcg acctcccgga ccggccgaac atcgacccgg acaagcggtt
    17761 cgggaccgac ccgacgccct acctgatcct gttcggggtc ggcttcctca cggccgtgct
    17821 aggccacatc gtcaaggtca aggtgatggt cgccgcgggg atcctgatgg tcttcctcgc
    17881 gacggtgatc ctgccgctcg tggcccgctc gggttactag ctgggcgacc gactccatcc
    17941 cggagccgag cgtcttgagc gcacggtgct ggatgtgccg caccgcatcg ggcgtggacc
    18001 cgacgacgtc gccgatctcg gctgtgctga acccgaacac gaagcgcagg gtcagcacgc
    18061 gccgctgcag ctcgggcagt ccgtcgatca gcgaccggac gccgctctcc ggatcgagcc
    18121 ggtcgagcag cgaggccgcg cgatcggcca ccggcagcgc gtgggcgtgg atcgagcgga
    18181 actcgacctc ctcggcgcgg gcgccctgcc tcaggtggtc gatggccacg ctgcggacca
    18241 tgctgaacag ccagtcgcgg aactcgccgc gccggtggtc gaagcggtcg agccggcgca
    18301 gcgcccgggc gaagacctcc tgcgcgacct cctgggcgtc gtcggggctc ttgagtgcga
    18361 tcagcaggta gcggtgcaca cggtcgaaga actcgacgta gagctcggcg aacgcgaggt
    18421 cgtcgcccgc ctgcgcgcgg cgagccagcc gcgacgcacg cgagccggag cgggagtcgg
    18481 aggagcgctc gacggagcgc aggatcgaat gggtccggtg gcgctcatcc ccggcgcgga
    18541 cgagtggagt cccccggccg gcggccctcg attccgcgcg ctcgggaggt gcggcgccct
    18601 tcgacgccca gcttcccccg acccgcatgc ctgactgatc ttcgctcaag acggctccgg
    18661 cgctacaggc actgtgcctg gagctttccc ggcagccgcg ccgcgcaatc agggtgtaac
    18721 gcgtggtgac ccgcagcggg gacaggggaa cccgcaaatg gcgtcgtccg aaaaaaaatc
    18781 gcgtggtcgc gtcatgaatc gagcctggcc cacgtacttc ggatcagttc gcactactag
    18841 gggggcttcg attctggact cgctcgagcc gccgcgggac gtcgcacgtt cccgggcggt
    18901 ccatccacgc ctgctcgcgc catgagggcg gagctgcgca ggctcgtact cgcggccgcg
    18961 ctggcgctcg cgatcttcgc cgccgcgctc ggcacggcca ccggcgagac gagcgaccgc
    19021 gaatcgcgat cgagccgcgc gagcatcgag ggatccgagg cacgcgacgc gtgggcggag
    19081 gcccgcgact ccgagcagcg ccgccgcgct catcgcgact cggccgacgg gcgcgaggag
    19141 cggtcgcggt cgcggaccgc gttccgcaac cagccggacg accaggcgct ggccatcgcg
    19201 cgcaagaagt tcccccggct cgtggacgcg ccggcgctgg agtggccgga cctcgggccg
    19261 gggcgcaagc tcgagcgcta cctctcgtcc aaccgcgcgc tcgttgacga gcccggcggc
    19321 cggaagagcg tcatcgagag caccctgccg ctcatcggcg agagcccgag cggcgacaag
    19381 gctccgatcg acctcgccct cgaggaccgc ggcagctcgt tcgctccgaa gagcagcggt
    19441 gccgcgatcc gcatcccgaa gggtgcgcgc ggtgcgttgc gcttcctcga ccaggacttc
    19501 ggcatcgcac tcgacggcgc gcgcgccggt gacgcgaagc tcagctccaa caaggcgttc
    19561 tacgcgaacg cgctgccgga cgccgacctg gtgatcgagc cggtcccggt gggtgccgag
    19621 atatcgctcg tcctgcgctc gccggacagc ccgaccaagc ccgcgctggc gttcgacctt
    19681 ccgcccgacg cgcggctgcg gctcgtcgcg catgcggact ccgactccgt ggcgccgggc
    19741 tcggccgagg tcgtgcgcga cggcaagcgc gttgccctcg tcccgcccgc gcaggcgctc
    19801 gactcgcagg gcgagtcggt gccggtgggg tacgaggtcg acggcgaccg gctggtcatg
    19861 cgcgtcgaca ccagcggcga gatcgcatgg cccgtggccg tcgatccgac cacctacatc
    19921 tacgacaaca acggccagag cacgtgcccg cccggcggtc cgctcccgtg cgacgggccc
    19981 ccgaccggct tcaactggcc gggctggacc ccgtggagca gcagggcgca gacaaacccc
    20041 tgcccgtacc ccagctcgca gtggttctac gagtgcaagt ccagcgacgg cgcgctctac
    20101 gtgtggggct tcaccggctt caactacgcg gcgaacgacc gcgcgctctg gcagaagacg
    20161 gctcgcccgg gctcctacat cttccgcttc gacgcagcca acatgagcca tacggccagc
    20221 gcgagccagc gcttctcggg catctgcggc gggcccgggt gcaccggctg ggcggcgtcg
    20281 ggccagtggt tctcgaacga cccgaactcc tgggcgaaca ccccgtaccg ctactacggc
    20341 gcctccgaga tgagcaggac ggagtactac tgcgtcaact cggtgcacgg ccaggcctgc
    20401 accccgcagc aggcggccac cgactcgagc ctgctcaaca actcggtcat cttcgggatg
    20461 atcatgaacg gcgggtacgc ctcctcgcag ccccatgtcg cgatgggcgg ggcggcgacg
    20521 tactcgtccg acaacgtggc cccgaacctg acggtggtcg cccacgaccc cgagctgccg
    20581 gccggctggg tcgagggcta cacgggcacg gtgcgcttgc gtgcggtcga cggcgcgggg
    20641 gcgaacaacg gcctgggcat gggcacgctc gcgctcaccg gcgggatcgg tcagaacccg
    20701 ctctccacct gcacgaccaa caacgcctac gacacctgcc cgaccacctg ggagcccacc
    20761 gcggggctcc cctacacgac gccgccgacc gacggcacct acagctacac cgcgagcgcg
    20821 accgacatcg tgggcaaccg caacacggcg caggacaagg ggtggtcgat caaggtcgac
    20881 cgcaccccgc cgggtgccta cgccaccgag cgcgcccgct gggtcaagga cggggtccac
    20941 cagttcacgg ccacctccag cgacagcctc tccggcgtgc gccagagccg gatctgggtc
    21001 gcgcgcgcct ccgaccgctt cgagcggacc gcctccaacg gctgggggac ggccgaccag
    21061 ggcggggcct ggaccgtgga cgagggcccg gcgagcgact tctcggccag cccgtccacg
    21121 ggcggcaaga tcgacttccc ctccggcggc gggttccgct cggcgtcgct ctacgggcgg
    21181 caggtcagga acgcggacgt gagcgcgcgg atcaagctcg gacagccgac ctccgggacc
    21241 ggggtaggga gcttcgccaa cctgatgctg cgccggcagc cgaacggcgg cgacaactac
    21301 cgcgtcgggc tgatcggcac caacgacggc cgcctgctcg tgaggggagc gcgcaacgcg
    21361 ggcaccttca tcgaccagga caccgggctc gggttcacgc ccggcgactc ctaccggctg
    21421 cgcgtggtcg ccctgagcga tgcggccggc accgccacga cggtctacgt caggtactgg
    21481 cgcgacggcg atccggagcc ggcgacctgg aacatcacca ccgcgccgga cagcgccggg
    21541 ccgcagggcg cgggcgccgt cggcgtgcgc gtgaacggcg gctcgacgac cgctcagcag
    21601 ttctacttcg acgagatcaa gctcgtcgat cgcgacacga ctcagacgaa ggacgcgacc
    21661 aaggccgacg gaaccccgtg cgattcggtc tccggctgcc cgcagtcgct cgcccacacc
    21721 taccagctca acaccgcggg ctattccgag ggcccgatcg agatcgtcgg cagcccccag
    21781 gacccgctcg cgggcgcgcc caacacgtcg tacgaccgcc accagcgctg ggacgactgg
    21841 cagatgaagc tcgaccggac cgggccgacg ttcgacgccg ctccgtcgcc gaacccgccg
    21901 tacgccaacc tccggcccgg cgagaactac gagttcacgg tcgattccag ggacaacctg
    21961 tcgggcgtcg agaagatcga gctctacgtg aacggcgtgc tgacgggccc gtccgtcccg
    22021 ggcgacaact gcagctccgc cggctgcgac acgaaccctt cggccagggg gatgagctac
    22081 cagatcccgg ccgactactc cggctcgaac gtgacgttca agctgatcgc caccgacaac
    22141 gccgggaacg agagcgcccc gcacgtatgg cgcgtgaaca ccgacgacga cccgccgacg
    22201 gctccggtca tcagcggcgc gctgcgggcc gcgcagggca aggcggtcaa gggc
//
LOCUS       DASVPL010000285        25420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_269918, whole genome shotgun sequence.
ACCESSION   DASVPL010000285 DASVPL010000000
VERSION     DASVPL010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 25420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25420
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269918"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VF517_15900"
     CDS             <1..510
                     /locus_tag="VF517_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054471.1"
                     /translation="AHEHRHGSIEHRHLHPHGGHGAGAGHGAGAGHGAGAEHGAAHEH
                     GGSGHEHAHAHAAHDHRHEPEARLGRTRLQAFGIGLVHGAGGSAGLGVLMLAAIPGQA
                     EAVAALMVLALGTAISMALVSSAFGYAITRGPVLRRMLVLAPAMGVVTMALGAWYLLG
                     AVGAVPYGL"
     gene            complement(529..1527)
                     /locus_tag="VF517_15905"
     CDS             complement(529..1527)
                     /locus_tag="VF517_15905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEX8054472.1"
                     /translation="MTLERARAGDEGAFRELTDPHRRELQLHCYRILGSIQDAEDLVQ
                     ETLLAAWRSLAAFEGRASVRSWLYQIATNRCLNALRDRSRRPREELQNMTLPEPTRRT
                     EPIRLDPYPDALLEQLPDSSPGPAARYETRESIELAFVAALQHLPSRQRAALVLRDVL
                     GFHTAEVAEMLDTGEASIKGVLQRARATTRAGSPASDRERAPQPSSARERHLVGRFAD
                     ALQSGAVDDVVALLTDDALLTMPPQPLEYEGREAIAAFIRNREEVRGAPLRLVPTRAN
                     TQPAFGCYIPDAQAAVARPAGMFVLTLAGDAVAALTWFADTSVFPRFGLPQTLPNP"
     gene            1663..3129
                     /locus_tag="VF517_15910"
     CDS             1663..3129
                     /locus_tag="VF517_15910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8054473.1"
                     /translation="MVLVLACAAQFMVVLDIAIVNVALPSIQRDLGIGQSTLQWIVIA
                     YGLMLGGFLLLGGRMADLLGRRRMLLTGLTLFSAASLLAGVAGSAALLIGARALQGFG
                     AALIPPAALSILAVTFAEGAERNRALGLYGAVTGVSASVGVIASGLLTDGPGWHSVFL
                     INVPVGLVLIAMAAVFLSVDARDRRATERFDIAGAVTVTGGLLLLVYALNRAADAGWA
                     SGSTLGLFAASVVMLAAFVRIEARSRSPLVPGSAVRNRTLVAANLSAFFTFGAFFAFI
                     FLGSLLMQQVLGYSPAQTGVAWLATTATSFVAAAITGGKLVAVVGVRRLLVAGQSLLA
                     ISVLLMTRVPAGGDFATDLLPALLLAGLAGGIAAPAAQIGALSGVTHSMTGLASGLVE
                     TMREIGGAVGVAAVSTVLVSRAGEIAGAADPAAREAAAANGFHVAFWVIFGVAALGAL
                     AAALAFPLTAPDNRRTEHGKDRDQRERLARRSRPGPNR"
     gene            3065..3637
                     /locus_tag="VF517_15915"
     CDS             3065..3637
                     /locus_tag="VF517_15915"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8054474.1"
                     /translation="MGKIVISENVSLDGVVQDPTGDEGFRHGGWVGLIKDRPELGKLT
                     LDEALGAEALLLGRRSYEWFGARWPSRSGELADRLNSLPKHVVSSTLQDPAWNNSTVL
                     RGDAVDEVTRLKQELDGEIVVYASFQLVRVLMEHDLIDEVRLKVFPVVLGDGERLFGA
                     TSDRKPMRLLETRTLDGGFAYLTCELVRET"
     gene            complement(3653..4717)
                     /locus_tag="VF517_15920"
     CDS             complement(3653..4717)
                     /locus_tag="VF517_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8054475.1"
                     /translation="MSLFNGGGGGRDLAIDLGTANTLVYARGRGIALSEPSVVAIDSD
                     TGEVHAVGDEAKRMIGRTPASISAIRPLRHGVIADFEVTERMLHYFIHRVQSSRFGRS
                     RVIMCVPSSVTEVEKRAVEEACMSAGASRVHLIEEPVAAAIGAELPIAEPRGSMIVDI
                     GGGTSEVAVLSLGAIVVSESLRIGGYDFDDAITLHMKRAHGMAIGQRTAEDMKLELGS
                     ALELQEELSGDVRGRDLVSGLPRTVKITSQELRVAYAEQLQAIVDTVHETLERTPPEL
                     ASDISESGIVLAGGGALLKGFDVLLADETQLPVRLADSPLTCVAVGAGRSLEEFDTLE
                     RMGNGRRRQLGRTGRTSFAR"
     gene            4761..5726
                     /locus_tag="VF517_15925"
     CDS             4761..5726
                     /locus_tag="VF517_15925"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8054476.1"
                     /translation="MPGRRIFISYRRGATTIYAGRLHDSLCMRLGDEEVFMDVDAIEP
                     GADFVDHIEREVGSCEILIALIGRDWIDATDDEGRRRLEDPDDWVRLELETGLNRDIR
                     VIPVLVQGATMPRAQELPGDLAKLARRNALEIGDTRWRYDFDRLMEVVEKVLEGNSVP
                     KAIELSGVRNFVPPPAGWQAGDRGAREPDARSPDAEPEPGAGAADDDEGIALVFVQEK
                     PVAGGEHRIEDGMTIGRTDGDVLLPDPQVSRRHAVIRLDDDSHPVIEDLGSTNGTYVN
                     EEPVTGPRRLEVGDVVRFGAVAWRLKPAPEGTKVGPRPQGSAGCA"
     gene            complement(5702..7408)
                     /locus_tag="VF517_15930"
     CDS             complement(5702..7408)
                     /locus_tag="VF517_15930"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HEX8054477.1"
                     /translation="MRWTVLAGLTVVAAVLGYVGFERLPDGDAWTFWDSFHRTVQLFV
                     LESGGVSPPVPWQLDVARFLAPALTVAAAAVALLSLFREQARLLMVRLRTRDHAVVAG
                     LGSKGWALARQLPEGGVVVVEQDPAAPAIAGCRERGIPVVVGDATDPAVLARAALGRA
                     RRLFVVCGEDGVNADVASAARGAGGDLGVHVHLDDLDLWRHLVGHELAEGARSSVRVE
                     FFNLADAAARMLLEAHPPFAPGAAEAHVVVSGVSGAGESVVLHAARAWLGGRPDADAR
                     MRVTACGPAAEADAARLLARHPRLAGVCDIRTWTLHSAEGATPPAGLTAVTAAYACDS
                     PDARAVSIALALRASGALGEVPAVAMVRDESAGIASALHEGGGVVAFGLLDRAPLAEV
                     ATQGTTEVLARAKHEQYARDERRRGRTPEENPSMAPWHELGESLRQSNRRFAEGVGEK
                     LRAARIAVVPAPLADPGAPATALPEEQVEELAVAEHDRWCADLVADGWRHGPGAKDPE
                     RKLHPSLVPWEELSEEEREKDREPVRALPEMLAHVGFELRPAGPAEAPAGAGRYAQPA
                     LP"
     gene            complement(7437..7919)
                     /locus_tag="VF517_15935"
     CDS             complement(7437..7919)
                     /locus_tag="VF517_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054478.1"
                     /translation="MKLLAAILIGLALVTAGCGGDEKDEPEQPAQTQTQTAPEPATTD
                     SDPAPAPADASEDDREAVQDVVKKWLIEGGCERMTDKFLEDQLLGLGDNRKERCDLFE
                     KQHTKPQYGEDQIKISAVQVSGTKASLVVGSDNAPDITSKYELVKTGEQWQIDSAEIQ
                     "
     gene            7964..8803
                     /locus_tag="VF517_15940"
     CDS             7964..8803
                     /locus_tag="VF517_15940"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8054479.1"
                     /translation="MPAPFTKTIRGAEVPTVGLGVFEVERHEAADTVADAIAAGYRHV
                     DTATAYGNEREVGEGIRRSGVDRAEVWVTTKVWIDDLPPDRLRRSAEGSLRRLGLDRV
                     DLLLIHWPPADPGAVPPALDELRRLREEGLVREFGVSNFPGYMLREALGHAPEIFADQ
                     VEYHAKLSQGELRELAAEHDLLLEAYAPLGGSASKMVGEPLLKEVAAEHDATPAQVAL
                     AWLARQERVVLLPRSTDPNRRRENLAALELDLGDEDVRRLDELSERRERYFDPSFAPD
                     WRD"
     gene            8868..9695
                     /locus_tag="VF517_15945"
     CDS             8868..9695
                     /locus_tag="VF517_15945"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX8054480.1"
                     /translation="MRRHGEVESSGGLGLHAHGCWTYGDDAEFRAGVFDFLVDGLELG
                     QRLLYVGPGGVAKLRRDIDDIPGVESLLADGTMRIMPLETAYAVGTPIDPIAQLTMYA
                     AATETALADGFAGLRVAADVTPLVTNPALWEAHLRWEGVADRYAARHPLAGLCCFDRR
                     QLPDEIVADLGCVHRATHGAAALAPFRLFAGREGLSLAGQVDCFCADDLRRLLRLASP
                     QEGPLVLELDELDFIDHHGVFAIADHAEELTRSGRWMSVRGAPPSFDRLVELLRVEL"
     gene            9692..10621
                     /locus_tag="VF517_15950"
     CDS             9692..10621
                     /locus_tag="VF517_15950"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8054481.1"
                     /translation="MSATAQRFHHEALLYEGIDGFMASTLPFVREGVARREPTLVAVD
                     ESKIALLKEALGEDARSVQFAEMRRLGRNPSCIIPAWRQFVIDNAGDAVPMRGVGEPI
                     WPGRSAAELDECHRHESLLNVAFDDGPAWTLICPYDTAALPADVVEHARTTHPLVGAD
                     GHSHPSHAYCDPYADPLGGELPAPAAPVEEVEFRLADLRTVRDLVSAWARSAGLGEKR
                     AADVVLAVSELAANSVRHGGGRGLVRSWREPGRFFCEVRDTGRIGDPLAGRGVPLEET
                     AGGRGLWIVNQVCDLVQLRSGESGSVARLHLAL"
     gene            10709..11947
                     /locus_tag="VF517_15955"
     CDS             10709..11947
                     /locus_tag="VF517_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054482.1"
                     /translation="MRTRTRNRTIAAACALAACALGGAAASSPAQDRADLQTTDLISR
                     SLSGGVPNGPSTNAVISGDRRYARLIAFESTASDLVAGDTNGQKDVFAIERTGSYSNV
                     GEEWRPGGTRLVSRGLGGAPANGPSWGAAVDGDFDNGQASCVAFLSSASNLVKGDTNG
                     RVDAFLVKGGGSPRRVSLPGNEQSGADTTAVDVSGDCSRTAFVIDGRLYVRSSRTKKL
                     RHSKPAADPAFAEGRSNDIVFGAPEGVFLARGGAYKPSLVAPGGRNPVFNNIKRQVVA
                     YERSLGGSTQVVFKDLGQNEVVASARDGRHGNHDSHNPVIGNSGYYITFESHADNLGT
                     TSTSRLLDDNDALDVYLYTDVRKITLAQSVFEKGVPVSGGGQSPSMSFYANYIVFHAN
                     SPLGSRRGTPQIYMRYLGPV"
     gene            complement(11952..12857)
                     /locus_tag="VF517_15960"
     CDS             complement(11952..12857)
                     /locus_tag="VF517_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054483.1"
                     /translation="MKVGVIVLAIGVIAVVGAIAFYMLAMPGRLAADYKEDAEPAQKE
                     LRSAMRPVFRTLSPETFGFDARSVRKAKTPDQYVRAIDKATSSSLRRLEPAHTAIRRA
                     KRVLEDADEEALYETPDWPLLGGRGDLEDAEAIAEDERRYVSEARAFLKHYDRLLSYT
                     LDMANFVKGFGVAFGRGFASIPDRPTSPGQVTGPLGRTVSRLDREMRTLRRLKAPPEL
                     RADHKTQVAFVRFVIRQIDSLSSAVKARDLQRIEAWDNEFARGTRRYDTTVSLRRLVK
                     RSSYAKSVRKLRGTERRLLRGFERL"
     gene            complement(12910..13407)
                     /locus_tag="VF517_15965"
     CDS             complement(12910..13407)
                     /locus_tag="VF517_15965"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX8054484.1"
                     /translation="MLAESRTHDLSRLELRDLVHDLAGRPELWAHLVRHDPSERVYCE
                     LLRDDHVAVYLICWMDDHDTGYHDHDVSAGALAVASGQVREERLAIGNEPASTLAGPG
                     DVLDFAPEDIHRVTHAGRLPAVTIHAYSPPLRGMGAYDIADDGRLRRHAQSYEEELRP
                     RAALG"
     gene            13519..15009
                     /locus_tag="VF517_15970"
     CDS             13519..15009
                     /locus_tag="VF517_15970"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8054485.1"
                     /translation="MDDHAQSRREFLARTAYTAGLAGASSVLPANLLLKEAAAAQARR
                     VPLPRPRDVPIDHIVIVMMENRSFDHYFGWLGGVADATQRATFPNEQGQQIATRHAGQ
                     LEAPWQGCGHPDPGHGWNSGRAQLKGGFLAKGSGNDEFALTYYNEDDCGFIHPAGKAF
                     TVYDRFFCSTLTSTWPNRYYKWSAQAGGRKTNDPPVDTLGNQWETIFDRALASGVSAR
                     YYNSDLPFSVVWGPRAVPWTNKLERFYADCATGTLPNISIVDPPFRDGGGGEGNSADE
                     HPHGDVRLGQAWMSDIVHAFMASPNYKRGALFLVYDEWGGFWDHVRPPRVPDVRGDYK
                     NPDEDWGQMGFRIPAVAISPYTRKSGNARARVSHATLGFESIIKFISYRFGLGNLTIR
                     DRYAKNIGRTFDWGKPQFEPPDLPDPAQVASAPCPTGGDQTPVPETTAESKQEHANDL
                     AGLEALAYRFGVPVGDGKVEDIFTKPDALKRAIRKRQISPSGAIRR"
     gene            15047..15721
                     /locus_tag="VF517_15975"
     CDS             15047..15721
                     /locus_tag="VF517_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054486.1"
                     /translation="MKVRLLLTVCAVAGAIVALPAAPASAASKCSGKGAQGLASSGSS
                     RVFSLPSKGERKIYACLYSQNKRRFLGWFQECQNMTAAREFRLVGPYVAYVEYSCGLI
                     AGNDSVVVLNIKTGKVVHSAAAATGTEAPGGEATTTVTGLELAATASVVWIADFDADS
                     GGLGNNAQDDRQVRKFETGEAGPVTVDSGLTIDPESLSLAKRYGTTNRFYWMKGGSPF
                     TALLSE"
     gene            complement(15741..16556)
                     /locus_tag="VF517_15980"
     CDS             complement(15741..16556)
                     /locus_tag="VF517_15980"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX8054487.1"
                     /translation="MTTLEAIVLGLVQGLTEFLPISSTGHLRIVPAFLGWDDPGAAFT
                     AVTQLGTLTAVLLYFRNDLWRIAIAFLAGLRDPLIRKSTDSRLGWYILLGTIPISLFG
                     LVFNNQIEDEARSLRLIGTTLIVLGLVLLAAERVGKRERDLDDLGARDGVAIGFAQAL
                     ALIPGVSRSGATLTAGLFLNLERAAAARYSFLLSVPAVVLSGLYELKDIGGDGGAGAF
                     PTAVATLLAFVSGYFAIAFMLRFLATNSTIVFVVYRVALGALVLALVGTGAID"
     gene            16582..16860
                     /locus_tag="VF517_15985"
     CDS             16582..16860
                     /locus_tag="VF517_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054488.1"
                     /translation="MTRAIRYGAPGVLALAGLVLVLASDSRGADSAGYTLLGSSALLV
                     LSIVIFRAGLASNDDRDREEAARVFFDEHGRWPRRGELDALSGKDPGR"
     gene            16857..18374
                     /locus_tag="VF517_15990"
     CDS             16857..18374
                     /locus_tag="VF517_15990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8054489.1"
                     /translation="MRQLTSLDAGFLAMESPRTYGHVGGFAVHDPTTAPGGTVTRADL
                     CRLVGERIHLLPPFRWKLAPVPLGLDNPYWIEDADFDLDFHVRETAVPPPGGARQLGD
                     QIARIFARPLDRTRPLWELYLVHGLEGGKVGILTKIHHAAVDGMSGAEIMGILLDSSP
                     DGREVAPPDRDCGERAPGQLEMLGRGLLGVPRQPLRALRALPSALPGLDDVPTIRTIP
                     GVGQLGRAAFQAQSWARGSRDGGVLERSAVRAPRTRFNGRISPHRRFAFGSLPLDKVK
                     AIKNELGITVNDVVMALCATALREWLIERDELPRDPLVAMVPVSVRTPEEQGTYGNRV
                     STMIVPIPTNEPDPRARLMLMHDSMRAAKDRHSALPASLMQDATQFVPPALMSRAARV
                     IAELSEVGPPPLNLVISNVPGPQDPLYLAGSRLVANYPVSVIMDGVGLNITVLSYNGR
                     LDFGIVADREQIEDAWPLMRKLGESLDEIDETICRSGKTVSPVTHGPRSGSVAVS"
     gene            18376..18705
                     /locus_tag="VF517_15995"
     CDS             18376..18705
                     /locus_tag="VF517_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054490.1"
                     /translation="MRESWFRALVLYPLLFVGPWVALSWLLAEPEEDGLVAVLGVGFW
                     GVIAGSFASLVAAVLVATRRPMPGRERVLLVVLGVLVSGAALVLGMVGWFQAAKYDCA
                     DQYECPI"
     gene            complement(18710..19153)
                     /locus_tag="VF517_16000"
     CDS             complement(18710..19153)
                     /locus_tag="VF517_16000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8054491.1"
                     /translation="MPEAREIPAGESLLAAAALLELRPQYESAEAIARRVDEQRAEGY
                     RLAASFEPGDEDAAAVAGFRLSHSLSWGRHVYVDDLVTRAALRGRGHADAVMAWVEEE
                     ARRQGCEQLHLDSGLGLDRADAHRFYFRHGLVIASHHFTKRLTPG"
     gene            complement(19333..20367)
                     /locus_tag="VF517_16005"
     CDS             complement(19333..20367)
                     /locus_tag="VF517_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054492.1"
                     /translation="MEREESDLYGLPLDEFIPARTALEKQLRKEGERERAAAVKKLPK
                     PSVAAWAVNQASRCRPKERRALMDASAALREVQERLLTREATAADLERATTAQREAIG
                     GLLEAASGLLTSEGKSMGEATLTRVRETFAAVATDPELAELVEAGVLDRERQATGLGF
                     ALGAGPAAPARSKGSRGGKSSGKAAPNGSGKARGGGLAKAGSAKAGSAKAGSARSKGS
                     AGAGNGGTKGGGSKAAGKAGVKRETARAEAAEREAAERERREAAARAARERLKAAKKE
                     ESEAERRVEAAERATRKAAEELDKRERQVEEARARADSAREELEAASAARDEAAQARE
                     SAAAAAAELE"
     gene            complement(20460..22313)
                     /locus_tag="VF517_16010"
     CDS             complement(20460..22313)
                     /locus_tag="VF517_16010"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX8054493.1"
                     /translation="MATTPIAPTFKTFGGDGPQRASASHSRILEDRDDGTRILLERGM
                     SSAAVVEVQQELKVAEDGIFGPITERAVKRYQAKHGLPPTGKVDVRTWLTLFPDGMVV
                     YDPSNQSAQLAAQAPKQAVQGLAGANPAAPVSAASVGGPTGLAANGPAESDQDLRTAR
                     NQARDGGAPESAEADGGILPDRDAERSERKQRKRERRGSGHDRSANVTEGVTVDRGSR
                     GGSSGGGGGNAGSRGGSSGGGGGGGGGPVGGPINVSPGLGSAQEMIAAMVAAANKIDR
                     AHYSYRWGGGHNPSFSGPYDCSGAVSAVLHAAGLLNSPMVSGGFMRWGRPGPGAVTIY
                     ANAGHVYMSINGRFFGTTRANPGGGAGWFNGSRRAGFVVVHVPFEAMKFRKSTKRTVK
                     SASTTRTQTRAATASQEPSSSKASGSRDAQSGGATYQSTAPAEGGTTAEAQAAPEQGT
                     APAPAPEAAPAEAAPAAAAPAQAAPAPAAAPAEAAPAQAAAPEAAPAAAAPAPAAAPA
                     EAAPAAPAEAEPAAAAPAPAAPAAPAPAAEAPAPAAEAPAAPAPEAAAPAPAAPEAAA
                     PAEAPAPEAPAADPAPEAAAPADSTTDAAGSATESKDGSQDEQSGSAATSG"
     regulatory      complement(22467..22627)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            22871..>25420
                     /locus_tag="VF517_16015"
     CDS             22871..>25420
                     /locus_tag="VF517_16015"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEX8054494.1"
                     /translation="MRRSLLTILALALTLAAPASAQERDLASYADPMVGTGPPGFVFP
                     GAVVPFGMVQNSPDTRGEFAYSGYLYADPQIQGFSLVHISGPGVRKGGDIPLMPTVGP
                     VNSNDPVQYGSPFDHAREEAEPGYYRVHLAKYATDVELTASTRAAMQRYTFPPSPQSN
                     VIVDVGRSVEGVHSGQIRFTGPDEISGYSEGRYRVYFVAKFSRPFASTGTFKPDGEGA
                     GGWATFNTLTDRQVTARIGISFVDAAGARRNLEADAPTFDFDRMRARAREAWNRELGR
                     LNVSGGTDLERTTFYSALYHSSLHPNVFSDVDGRYMGFDKQPHRAEGRTQYANFSSWD
                     LYKSTNQLLATLQPRRYRDMLLTLLANYREWGRLPRWGEHYFDANHMSGDPAIPMIAD
                     GLCRGGILSHGEAEDLLEASNALYRRREASLQQLGYLPDRPGTTLEFGVADFALALLA
                     DAVGHDSEAKARLTDSLRYRTVLDPETRWVRPRHADGSWYEPFDPTDTTGFQEGNSWQ
                     YSWLAPHDARGLYDRMGGDAAAIERLDQYFSAPPEAANQLTFFGIVYKAPWHAPGNEH
                     DLQVPWMYPFVGQPWKTMSEHREIQRLFRPEPNGLPGNDDLGGLSSWLTWSMLGIGPV
                     TPGAPFYVIGSPTFERAVITPDGGSRFAIEAPGASPLRPYVTAASVGGRRLGSAWLYD
                     ASVRRGETVKLTVAEQPNTEFGSAPPVRPPSVSDSPLARFGCLPTAPQPDPVAPGAAE
                     RPAGFEDPRTAPVEPGPVLADGSAPPSLVPLRLRVSPRTVRTSRRTLYRLTVHAKRHG
                     RWVPVRGARVRLAGVRARTSGKGVAKIRATIRHHGTHRANATAAAARPAYRS"
BASE COUNT         3396 a         9289 c         9189 g         3546 t
ORIGIN      
        1 gctcacgagc accgtcacgg gtcgatcgag caccgccacc tgcacccgca cggcgggcac
       61 ggcgctgggg ccgggcacgg agccggggcc gggcacggcg ctggggcaga gcacggcgcg
      121 gcgcacgagc acggcggctc cgggcacgag cacgcgcacg cgcacgcggc gcacgaccac
      181 cgccacgagc cggaggcgcg gctcggccgc acgcggctcc aggcgttcgg catcggcctg
      241 gtgcacggcg ccggcggctc ggcggggctg ggggtcctga tgctcgccgc aatcccgggg
      301 caggccgagg cggtcgccgc gctgatggtg ctcgcgctcg gcaccgcgat ctcgatggcg
      361 ctcgtctcct cggcgttcgg ctacgcgata acgcgcgggc cggtcctcag gcggatgctg
      421 gtgctcgcgc cggcgatggg cgtggtgacc atggcgctcg gcgcctggta cctgctcggc
      481 gcggtgggag cggtccccta cgggctgtga cccgcggagc tatcaggctc aggggttcgg
      541 caacgtctga gggagtccga agcgtgggaa gacactcgtg tcggcgaacc acgtgagcgc
      601 cgcgacggcg tccccggcga gcgtgaggac gaacatgccg gcggggcggg ccaccgccgc
      661 ctgtgcatcc gggatgtagc agccgaacgc cggctgggtg ttggcacggg tgggaacgag
      721 acggagcggg gcgccgcgca cctcctcccg gttgcgaatg aaggccgcga tggcctcgcg
      781 tccctcgtac tcgagcggct gcgggggcat cgtgagtagc gcgtcgtcgg tgagcagggc
      841 cacgacgtcg tcgacggcac cgctctggag tgcgtcggcg aagcgcccga ccaggtggcg
      901 ttcgcgggcg gagctcggct gcggcgcgcg ttcgcggtcg gaggccgggg aaccggctcg
      961 cgtggtcgcg cgggcccgtt gcaggacgcc cttgatcgag gcctcgcccg tgtcgagcat
     1021 ctcggcgacc tctgcggtgt ggaagccgag aacgtcgcgc agcacgagcg ccgcgcgctg
     1081 gcgggacggc aggtgttgga gggcggcgac gaacgcgagc tcgatcgact ccctggtttc
     1141 gtagcgcgcg gccggcccgg gggagctgtc cggaagctgc tcgagcagag cgtcggggta
     1201 ggggtcgagc cggatcggtt cggtgcggcg ggtgggctcc ggcaaggtca tgttctgcag
     1261 ctcctcccgg gggcggcgcg atctgtcgcg gagtgcgttc agacagcggt tggtcgcgat
     1321 ctggtagagc cacgagcgca cggaggctcg gccctcgaac gcagcgaggc tccgccacgc
     1381 cgccagcagc gtctcctgca ccaggtcctc ggcgtcctgg atcgacccga ggatgcggta
     1441 gcagtgcagc tgcaactcgc gccggtgagg gtcggtgagt tcccgaaacg ccccttcgtc
     1501 gcccgcccgc gcgcgctcga gcgtcacctt gcctggctcc gtcggtccca tcaccactaa
     1561 ggacaccacc ttctcccgaa acggaccggc cgttccgttc tcaccgccgc cggtgtctca
     1621 gtcgacatgc acctggcaac gaaggcacac acgagcaaac gaatggtcct cgtactggcg
     1681 tgcgcggcgc agttcatggt cgttctcgac attgcgatcg tcaacgtcgc cctgccctcg
     1741 atccagcggg atctcggcat cgggcagagc accctgcagt ggatcgtcat cgcgtacggg
     1801 ctcatgctcg ggggcttcct gttgctcggc ggcaggatgg ccgacctcct ggggcgccgc
     1861 cgcatgctgc tgacggggct gacgctgttc tcggccgcct cactgctcgc gggcgtggcg
     1921 gggtccgccg cgcttctcat cggcgcccgg gccctgcagg gcttcggggc tgctctcatc
     1981 cccccggccg cgctctcgat cctcgccgtg acgttcgcgg aaggcgccga gcgcaatcgc
     2041 gcgctggggc tctacggcgc ggtcaccggg gtctcggcgt cggtcggcgt gatcgcgagc
     2101 ggtctgctga ccgacggccc aggctggcac tcggtctttc tgatcaacgt cccggtcggc
     2161 ctcgttctga tcgcgatggc ggccgtcttc ctgtccgtcg acgcgcgtga ccgccgcgca
     2221 acggaacgct tcgacatcgc cggcgcggtc accgtgaccg gaggtctgct cctgctcgtc
     2281 tacgcgctca accgcgccgc cgacgccggc tgggcgtccg ggtcgacgct cggactgttc
     2341 gccgcgtccg tcgtgatgct ggccgccttc gtgcggatcg aagcgcggtc gcgatcgccg
     2401 ctggtgcccg gctccgcggt ccgcaaccga acgctggtgg cggcgaacct ctcggcgttc
     2461 ttcacgttcg gagcgttctt cgccttcatc ttcctcggct cgctgctgat gcagcaggtc
     2521 ctcggttact caccggcgca gacgggcgtc gcctggctgg ccacgacggc cacgtcgttc
     2581 gtggcggctg cgatcaccgg aggcaagctg gtcgcggtgg tgggcgtgcg gcggctgctc
     2641 gtcgcgggcc aatcgcttct cgcgatctcc gtactgctga tgacgcgggt cccggctgga
     2701 ggggacttcg caaccgacct gctgccggcg ctgctcctgg ccggcctggc cggcggaatc
     2761 gcggctccgg ctgcacagat cggcgctctg tccggtgtca cgcactcgat gaccggtctg
     2821 gcgtccgggc tcgtggaaac catgcgcgag atcgggggcg ccgtcggcgt ggctgccgtg
     2881 tcgaccgtcc tcgtctcgcg ggccggtgag atcgccgggg cggccgaccc ggcggcccgc
     2941 gaagcggcgg cggcgaacgg tttccacgtc gcgttctggg tgatcttcgg cgtcgcggcc
     3001 ctcggtgcgc tggcggccgc gctcgccttt ccactgacag caccagacaa caggagaacc
     3061 gaacatggga aagatcgtga tcagcgagaa cgtctcgctc gacggagtcg tccaggaccc
     3121 aaccggtgac gagggtttca ggcacggcgg ctgggtgggc ctgatcaagg accgcccgga
     3181 gctcggaaag ctcacgctcg acgaggccct gggcgccgag gccctgctgc ttggccggcg
     3241 aagctacgaa tggttcggcg cgcggtggcc gtcccggagt ggcgagctgg cggacaggct
     3301 gaacagcctg cccaagcacg tcgtgtcctc gaccctccag gatcccgcct ggaacaactc
     3361 gaccgtgctc cggggtgatg cggtggacga ggtcaccagg ttgaagcagg agctggacgg
     3421 ggagatcgtg gtctacgcca gcttccagct cgtgcgcgta ctgatggagc acgacctgat
     3481 cgacgaggtg cggctgaagg tcttcccggt cgtgctcggg gacggcgagc gcctcttcgg
     3541 cgcgacgagc gacaggaagc caatgcgcct cctcgagact cggaccctcg atggcggctt
     3601 cgcctacctc acctgcgagc tcgtccggga gacctagcgg ccgcccgacc gtctagcgcg
     3661 cgaaggacgt gcgaccggtc cggccgagct ggcgcctgcg cccgttgccc atgcgctcga
     3721 gcgtgtcgaa ctcctcgagc gagcgccccg cgcccaccgc cacgcacgtc agcggcgagt
     3781 ccgccagtcg caccggcagc tgagtctcgt cggcgagcag cacgtcgaag cccttcagca
     3841 gcgctccgcc gccggcgagc acgatgccgc tctcggagat gtccgaggcc agctccggcg
     3901 gcgtccgctc cagcgtctcg tgcacggtgt ccacgatcgc ctggagctgc tcggcgtagg
     3961 ccacgcgcag ctcctgcgag gtgatcttca cggtgcgcgg gagcccggag acgagatcgc
     4021 ggccgcgcac gtcgccggag agctcctcct ggagctccag cgccgagccc agctctagct
     4081 tcatgtcctc cgcggtccgc tggccgatcg ccatgccgtg cgcgcgcttc atgtgcagcg
     4141 tgatcgcgtc gtcgaagtcg tagccgccga tccgcagcga ctcgctcacc acgatcgcgc
     4201 cgagcgacag gaccgcgacc tcggaggtgc cgccgccgat gtccacgatc atcgacccgc
     4261 gcggctcggc gatcggcagc tcggccccga tggccgcggc cacgggctcc tcgatcaggt
     4321 gcacccggct cgcgccggcc gacatgcagg cctcctcgac cgcccgcttc tcgacctcgg
     4381 tgaccgacga gggcacgcac atgatcacgc gcgagcgccc gaagcggctg ctctgcacgc
     4441 ggtggatgaa gtagtggagc atccgctcgg tcacctcgaa gtcggcgatc acgccgtggc
     4501 gcagcggccg gatcgccgag atcgaggccg gcgtgcggcc gatcatccgc ttggcctcgt
     4561 cgccgacggc gtggacctcg ccggtgtcgg agtcgatcgc cacgaccgac ggctccgaca
     4621 gcgcgatccc gcgcccgcgc gcgtagacca gcgtgttcgc ggtgccgagg tcgatcgcca
     4681 gatcgcgtcc tccgcccccg ccgttgaaca gggacacctg acgagcatag gtgtatgcgc
     4741 ccgacttaga ctcccctgca atgcccggac ggcgcatctt catcagctac cggaggggcg
     4801 cgacgaccat ctacgccggt cgcctccacg acagcctgtg catgcgcctg ggcgacgagg
     4861 aggtgttcat ggacgtcgac gcgatcgagc ccggtgccga cttcgtggac cacatcgagc
     4921 gcgaggtcgg gtcctgcgag atcctgatcg cgctgatcgg gcgcgactgg atcgacgcca
     4981 ccgacgacga gggccggcgc cggctcgagg atcccgacga ctgggtccgg ctggagctcg
     5041 agaccggcct caaccgcgac atccgagtga tcccggtgct cgtgcagggc gccaccatgc
     5101 cgcgcgcgca ggagcttccg ggcgacctcg ccaagctcgc ccgccgcaac gcgctcgaga
     5161 tcggcgacac ccgctggcgc tacgacttcg accgcctcat ggaggttgtc gagaaggtgc
     5221 tggagggcaa ctccgtgccg aaggcgatcg agctgagcgg cgtgcggaac ttcgtgccgc
     5281 cgcccgccgg ctggcaggcg ggggaccgcg gtgcccgcga gcccgacgct cgctccccgg
     5341 acgcggagcc ggagcccggc gcgggcgcgg cggacgacga cgaggggatc gcgctcgtgt
     5401 tcgtgcagga gaagccggtc gccggcggtg agcaccggat cgaggacggc atgacgatcg
     5461 gccgcaccga cggcgacgtc ctgctccccg acccgcaggt ctcgcgccgc cacgccgtga
     5521 tccgcctcga cgacgactcg caccccgtga tcgaggacct cggctccacc aacgggacct
     5581 acgtgaacga ggagcccgtg accggcccgc gccggctcga ggtcggcgac gtggtgcgct
     5641 tcggcgccgt ggcctggcgc ctcaagcccg cgcccgaggg gacgaaggtc ggcccccggc
     5701 ctcagggcag cgcgggctgc gcgtagcgcc cggcgcccgc gggagcctcg gccggccccg
     5761 ccggccgcag ctcgaagccc acgtgcgcca gcatctccgg cagcgcccgc acgggctcgc
     5821 ggtccttctc acgctcctcc tccgacagct cctcccacgg cacgaggctc ggatggagct
     5881 tgcgctcggg gtccttggcg ccgggcccgt gccgccagcc gtcggcgacg aggtcggcgc
     5941 accagcggtc gtgctcggcc accgccagct cctcgacctg ctcctccggc agcgccgtgg
     6001 ccggggcgcc gggatccgcc agcggcgcgg gcaccacggc gatccgcgcg gcgcgcagct
     6061 tctcgcccac gccctccgcg aagcggcggt tcgactggcg caggctctcg cccagctcgt
     6121 gccagggcgc catcgacggg ttctcctccg gagtgcgccc gcgccgccgc tcgtcgcgcg
     6181 cgtactgctc gtgcttcgcg cgcgcgagca cctcggtggt gccctgcgtc gccacctcgg
     6241 ccagcggggc gcggtcgagc agcccgaatg ccaccacgcc gccgccctcg tgcagggccg
     6301 aggcgatccc ggcgctctcg tcgcgcacca tcgccaccgc gggcacctca ccgagcgcgc
     6361 ccgacgcgcg cagggccagc gcgatcgaga ccgcgcgcgc gtccgggctg tcgcaggcgt
     6421 aggccgccgt gaccgctgtg agccccgccg ggggcgtggc gccctcggcc gagtgcagcg
     6481 tccaggtgcg gatgtcgcac acgcccgcga gtcgcgggtg gcgcgcgagg aggcgcgcgg
     6541 cgtcggcctc ggcggcgggg ccgcacgccg tcacgcgcat gcgcgcgtcc gcgtccgggc
     6601 gcccgccgag ccacgcgcgc gcggcgtgga gaaccacgct ctcgccggcc ccgctcaccc
     6661 cggagaccac cacgtgcgcc tcggccgcgc caggcgcgaa cggcgggtgc gcctcgagca
     6721 gcatccgcgc ggcggcgtcg gcgaggttga agaactccac ccgcaccgag gagcgcgcac
     6781 cctccgccag ctcgtggccc acgaggtgcc tccagaggtc gaggtcgtcg aggtgtacgt
     6841 gcacgccgag atcgccgccg gccccgcgcg ccgccgacgc cacgtcggcg ttcacgccgt
     6901 cctcgccgca gaccacgaac agccgccgtg cgcgcccgag cgccgcacgc gccagcaccg
     6961 cgggatcggt cgcgtcgccc acgaccaccg gtatcccgcg ctcgcggcag ccggcgatcg
     7021 cgggggcggc gggatcctgc tcgaccacca caacgccgcc ctcggggagc tggcgcgcca
     7081 gcgcccagcc cttgctgccg agcccggcca ccaccgcgtg gtcgcgcgtg cgcagccgca
     7141 ccatcagcag gcgggcctgc tcgcggaaga gcgacagcag cgccaccgcc gccgcggcca
     7201 ccgtcagcgc gggcgccagg aagcgcgcca cgtcgagctg ccacggcacc ggcggcgaca
     7261 ccccgcccga ctcgagcacg aagagctgca ccgtgcggtg gaacgagtcc cagaacgtcc
     7321 acgcgtcgcc gtccgggagc cgctcgaacc ccacgtagcc gagcacggcg gcaacgacgg
     7381 tcagccccgc gaggacggtc cagcgcatgg gcgaaagcta gcggcgccct acgtcgctac
     7441 tggatctcgg cagagtcgat ctgccactgc tcgccggtct tcacgagctc gtacttcgag
     7501 gtgatgtcgg gcgcgttgtc ggaccccacg acgagcgacg ccttggtgcc cgagacctgc
     7561 accgcgctga tcttgatctg gtcctcgccg tactgcggtt tcgtgtgctg cttctcgaag
     7621 aggtcgcagc gctccttgcg gttgtcgccg agcccgagga gctggtcctc gaggaacttg
     7681 tcggtcatcc gctcgcagcc gccctcgatc agccacttct tcacgacgtc ctggaccgcc
     7741 tccctgtcgt cctcggaggc atcggccggc gccggcgcgg ggtcggagtc ggtcgtggcc
     7801 ggctccggag ccgtctgcgt ctgcgtctgc gccggctgct cgggctcgtc cttctcgtcg
     7861 ccgccgcagc cggcggtgac gagggcgagg ccgatcagga tggcggcgag caacttcatc
     7921 ggcgggcgaa tgtagcgagg ccgttgcggg tagaggctgg ggcatgcccg cccccttcac
     7981 taagaccatc cgcggcgccg aggtgcccac cgtcggactt ggcgtgttcg aggtcgagcg
     8041 ccacgaggcc gccgacacgg tcgccgacgc catcgccgcc ggctaccgcc acgtggacac
     8101 cgccacggcc tacggcaacg agcgcgaggt gggggagggg atccgccggt cgggcgtgga
     8161 ccgggccgag gtgtgggtca cgacgaaggt ctggatcgac gacctcccgc ccgaccgcct
     8221 gcgccgcagc gccgagggga gcctgcgccg gctcgggctc gatcgcgtcg acctgctgct
     8281 gatccactgg ccgcccgcgg acccaggcgc ggtgccgccg gcgctcgacg agttgcgtcg
     8341 gctgcgcgag gagggcctcg ttcgcgagtt cggcgtgagc aacttccccg gatacatgct
     8401 gcgcgaggcg ctcgggcacg cgccggagat cttcgccgac caggtcgagt accacgcgaa
     8461 gctctcccag ggcgagctgc gcgagctcgc ggccgagcac gacctcctgc tcgaggcgta
     8521 cgcgccgctc ggcggcagcg cgagcaagat ggtcggcgag ccgctgctga aggaggtcgc
     8581 cgcggagcac gacgcgaccc cggcccaggt ggcgctcgcg tggctcgcac gccaggagcg
     8641 cgtggtgctc ctcccgcgct caacagaccc gaaccggcgg cgcgagaacc tcgccgcact
     8701 cgagctcgac ctgggcgacg aggacgtccg gcgcctggat gagctgtccg agcggcgcga
     8761 gcgctacttc gacccgagct tcgcgcccga ctggcgcgac tgagcgccgc cgcgccgcgc
     8821 cgccggggac gtcgccacag ggcgttggcg ctgccatact cgccgcgatg cgccgtcatg
     8881 gggaggtcga gtcgtctggt ggcctcgggc ttcacgcgca cgggtgctgg acgtacggcg
     8941 acgacgccga gttccgcgcc ggcgtcttcg acttcctggt ggacgggctc gagctcggac
     9001 agcgactgct gtacgtcggg cccggcggcg tggcgaagct gcgccgcgac atcgacgaca
     9061 tcccgggcgt cgagtcgctg ctcgccgacg ggaccatgcg gatcatgccg ctcgagaccg
     9121 cctacgcggt cggcactccg atcgatccga tcgcgcagct cacgatgtac gcggccgcca
     9181 ccgagaccgc actcgcggac ggcttcgccg gccttcgcgt ggccgccgac gtgacaccgc
     9241 tcgtcaccaa ccccgccctg tgggaggcgc acctgcgctg ggagggcgtg gccgatcggt
     9301 acgccgcgcg gcaccccctg gcgggactct gctgcttcga tcgccgccag ctccccgacg
     9361 agatagtcgc cgacctcggc tgcgtgcacc gcgccacgca cggggccgcg gcgctcgcgc
     9421 cgttccggct gttcgccggg cgcgagggcc tcagcctggc cggtcaggtg gactgcttct
     9481 gcgcggacga cctgcgccgt ctcctgcgcc tggcatcacc gcaggagggc ccgctggtgc
     9541 tggagctgga cgagctcgac ttcatcgacc accacggcgt gttcgcgatc gccgatcacg
     9601 ccgaggagct cacgcgcagc ggccgctgga tgtccgtccg cggcgctccc ccgagcttcg
     9661 accggctcgt cgagctgctc cgagtcgagc tatgagcgca acggcgcaac gcttccacca
     9721 cgaggcgctg ctctacgagg gcatcgacgg cttcatggcg tccacccttc cgttcgtgcg
     9781 cgagggcgtg gcgcgccgcg agcccacgct ggtggcggtc gacgagagca agatcgcgct
     9841 gctgaaggag gcgctcggcg aggacgcgcg ctcggtgcag ttcgccgaga tgcgccggct
     9901 cggccgcaac ccctcctgca tcatccccgc ctggcgccag ttcgtgatcg acaacgccgg
     9961 cgacgcggtg ccgatgcgcg gcgtgggcga gccgatctgg cccgggcgca gcgccgccga
    10021 gctcgacgag tgccaccgcc acgagtcgct gctgaacgtt gcgttcgacg acggcccggc
    10081 ctggacgctc atctgtccgt acgacacggc cgcgctgccg gcggacgtgg tcgagcacgc
    10141 gcgcaccacc cacccgctgg tcggcgcgga cggccacagc caccccagcc acgcctactg
    10201 cgacccgtac gccgacccgc tcggcggcga gctgccggcc cccgcggcgc cggtcgagga
    10261 ggtcgagttc aggctggcgg atctccgcac ggtgcgcgac ctcgtctccg cctgggcgcg
    10321 cagcgccggg ctgggcgaga agcgcgcggc ggacgtcgtg ctcgcggtga gcgagctggc
    10381 cgccaacagc gtgcgccacg gtggcgggcg cggcctggtg cggtcctggc gcgagccggg
    10441 ccgcttcttc tgcgaggtgc gcgacacggg ccggatcggc gacccgctcg cggggcgcgg
    10501 ggtgccgctc gaggagacgg cgggcggccg cgggctctgg atcgtgaatc aggtctgcga
    10561 cctcgtccag ctccgctcgg gcgagtcggg ctccgtcgcg cgcctgcacc tggcgctcta
    10621 ggccaggcat tacctcctca caacggcgtc cttgccacgt cttaacgaag gacgccgatg
    10681 ctcctgccga taaaggagtg gaccgccgat gaggactcgc acccgcaatc gaacgatcgc
    10741 cgccgcctgc gcactcgccg cctgcgcgct gggcggggct gccgccagct cgcccgcgca
    10801 ggaccgcgcc gatctccaga ccaccgacct gatctcgcgc tcgctcagcg gcggcgtgcc
    10861 gaacggcccg tccaccaacg ccgtgatctc cggcgaccgc cgctacgcgc ggctgatcgc
    10921 gttcgagtcg accgcgagcg acctcgtcgc cggcgacacc aacgggcaga aggacgtgtt
    10981 cgcgatcgag cgcaccggga gctactcgaa cgtcggggag gagtggcggc cgggcggcac
    11041 acggctggtc tcgcgcggcc tcggcggcgc gcccgccaac ggcccatcgt ggggcgccgc
    11101 cgttgacggc gacttcgaca acgggcaggc gagctgcgtg gcgttcctct cgtccgcctc
    11161 gaacctcgtg aagggcgaca ccaacggcag ggtcgacgcg ttcctcgtca agggcggcgg
    11221 cagcccgcgg cgcgtgtcgc tgccgggcaa cgagcagtcc ggcgcggaca cgacggcggt
    11281 cgatgtctcg ggcgactgct cgcgcaccgc gttcgtgatc gacggccgcc tctacgtgcg
    11341 cagcagccgc accaagaagc tgaggcactc gaagccggcc gcggatcccg cgttcgccga
    11401 gggccgttcc aacgacatcg tcttcggcgc gcccgagggc gtgttcctcg cgcgcggtgg
    11461 cgcctataag ccgagcctcg tcgcacccgg cgggcgcaac ccggtcttca acaacatcaa
    11521 gcggcaggtg gtcgcctacg agcgctcgct cggcgggagc acgcaggtcg tgttcaagga
    11581 cctcggccag aacgaggtgg tggcgagcgc gcgcgacggc cgtcacggca accacgactc
    11641 gcacaacccc gtgatcggaa actccggcta ctacatcacc ttcgagtcgc acgccgacaa
    11701 cctcggaacc acctcgacca gccggctgct cgacgacaac gacgcgctcg acgtctacct
    11761 ctacaccgac gtccgcaaga tcacgctggc gcagtcggtc ttcgagaagg gtgtccccgt
    11821 gagcggcggc ggccagagcc cgagcatgag cttctacgcg aactacatcg tgtttcacgc
    11881 gaactcgccg ctcggctcgc gccgcggcac gccgcagatc tacatgcggt acctcgggcc
    11941 ggtctgagcc gctacaggcg ctcgaacccg cgcagcaacc ggcgctcggt cccgcgcagc
    12001 ttgcgcacgg acttggcgta gctcgagcgc ttgacgagcc gtcgcagcga gacggtggtg
    12061 tcgtagcgcc gcgtgccgcg cgcgaactcg ttgtcccacg cctcgattcg ctggaggtcg
    12121 cgcgccttca cggccgagga gagcgagtcg atctgccgga tcacgaaccg cacgaaggcg
    12181 acctgggtct tgtggtcggc gcgcagctcc ggcggtgcct tcaggcgccg cagggtccgc
    12241 atctcgcgat cgaggcggct gacggtgcgt ccgagcggtc ccgtcacctg cccgggcgag
    12301 gtcgggcggt cgggaatgga agcgaacccg cgcccgaacg cgacgccgaa tcccttgacg
    12361 aagttcgcca tgtcgagcgt gtagctgagg aggcggtcgt agtgcttgag gaaggcgcgc
    12421 gcctcgctca cgtagcggcg ctcgtcctcc gcgatggcct cggcgtcctc gaggtcgcca
    12481 cggccgccga gcagcggcca gtccggcgtc tcgtagagcg cttcctcgtc ggcgtcctcg
    12541 agaacgcgct tcgcgcgtct gatcgccgtg tgcgcgggct caagccgccg cagcgaggac
    12601 gacgtggcct tgtcgatcgc gcgcacgtac tggtcggggg tcttcgcctt ccggacgctg
    12661 cgggcgtcga acccgaaggt ctccggcgac agcgtccgaa acaccggccg cattgcggag
    12721 cgcagctcct tctgcgccgg ctcggcgtcc tccttgtagt ccgcggcgag ccgtccgggc
    12781 atcgcgagca tgtagaacgc gatcgcgccc accaccgcaa tcacgccgat cgccaacacg
    12841 atcaccccca ccttcacggc tggcgtataa ccccaaatcg cggtgactaa cccgcggatt
    12901 ggcgcagcgc tagccgagcg ccgcgcgcgg cctgagctcc tcctcgtacg actgcgcgtg
    12961 gcggcggagg cgaccgtcgt cggcgatgtc gtacgcgccc atcccgcgca ggggcgggga
    13021 gtaggcgtgg atcgtgaccg ccgggaggcg ccccgcgtgg gtaacccgat ggatgtcctc
    13081 gggcgcgaag tcgagcacgt cgccgggtcc cgccagggtg ctcgccggct cgttcccaat
    13141 cgcgagccgc tcctcgcgca cctgccccga cgccacggcc agcgcgccgg ccgacacgtc
    13201 gtggtcgtgg tagccggtgt cgtgatcgtc catccagcag atcaggtaga cggcgacgtg
    13261 gtcgtcgcgc agcagctcgc agtagacccg ttcgctcggg tcgtggcgga cgaggtgggc
    13321 ccagagctcc ggacggccgg cgaggtcgtg caccaggtcg cgcagctcga gccgtgacag
    13381 gtcgtgggtg cgggactcgg cgagcatgac ggggacatat tatcgacaaa accggtcggg
    13441 attaaagagt ttcctgcatc gcgggcgctg tgacggcccg ttagcgcctc cggcggagca
    13501 gtcctgtaga acccgcacat ggacgaccac gcgcagagca ggcgcgagtt cctcgcgcgc
    13561 accgcctaca cggccgggct cgccggcgca tcctcggtgc tgccggccaa cctgctcctc
    13621 aaggaggccg ccgcggcaca ggcgcggcgc gtgccgctgc cgcgcccgcg cgacgtgccg
    13681 atcgaccaca tcgtgatcgt gatgatggag aaccgctcgt tcgaccacta cttcggctgg
    13741 ctcggcggcg tggccgacgc cacccagcgc gcgaccttcc cgaacgagca gggccagcag
    13801 atcgcgacgc ggcacgccgg gcagctcgag gcgccgtggc agggctgcgg ccaccccgac
    13861 cccggccatg gctggaacag cggccgcgcc cagctcaagg gcggcttcct cgccaagggc
    13921 agcggcaacg acgagttcgc gctgacctac tacaacgagg acgactgcgg gttcatccac
    13981 cctgccggca aggcgttcac cgtctacgac cgcttcttct gctcgacgct gacctcgacc
    14041 tggccgaacc gctactacaa gtggtcggcg caggcgggcg gccgcaagac caacgacccg
    14101 ccggtcgaca cgctcggcaa ccagtgggag acgatcttcg accgggcgct cgcgagcggg
    14161 gtgagcgcgc gctactacaa ctccgacctg ccgttctcgg tggtctgggg gccgcgcgcg
    14221 gtgccgtgga ccaacaagct cgagcgcttc tacgccgact gcgcgaccgg cacgctgccg
    14281 aacatctcga tcgtggaccc gccgttccgc gacggcggcg ggggcgaggg caactcggcc
    14341 gacgagcacc cgcacggcga cgtccggctc gggcaggcgt ggatgtcgga catcgtccac
    14401 gcgttcatgg cctcgcccaa ctacaagcgc ggcgcgctgt tcttggtgta cgacgagtgg
    14461 ggcggcttct gggaccacgt ccgcccgccg cgcgtgcccg acgtgcgcgg ggactacaag
    14521 aaccccgacg aggactgggg ccagatgggc ttccgcatcc cggccgtcgc gatctccccg
    14581 tacacgcgca agagcggcaa cgcccgcgcg cgcgtgagcc acgcgacgct cggcttcgag
    14641 tcgatcatca agttcatcag ctaccgcttc gggctcggga acctcacgat ccgcgaccgc
    14701 tacgcgaaga acatcgggcg cacgttcgac tggggcaagc cgcagttcga gccgcccgac
    14761 ctgccggacc cggcgcaggt ggcctcggcg ccgtgcccga ccggcggcga ccagacgccg
    14821 gtcccggaga ccaccgccga gtcgaagcag gagcacgcga acgacctcgc gggactcgag
    14881 gccctggcgt accggttcgg cgtcccggtc ggcgacggca aggtggagga catcttcacg
    14941 aagcccgacg cgctcaagcg agcgatccga aagcgccaga tttcgcccag cggcgcgatt
    15001 cgccggtagt ctgccggccc tcttcaggcg accaaggggg cgggggatga aggtgcggtt
    15061 gctgttgacg gtgtgcgctg tcgcgggggc gatcgtcgcg ttgccggcgg cgccggcttc
    15121 ggcggcgtcc aagtgcagcg ggaagggcgc gcaggggctc gcctcgagtg ggagctcgag
    15181 ggtgttctcg ctgccctcga agggcgagcg caagatttac gcgtgcctct actcgcagaa
    15241 caagcgccgc ttcctggggt ggttccagga gtgccagaac atgacggcgg cgcgcgagtt
    15301 caggctggtc gggccgtacg tggcgtacgt cgagtactcg tgcgggctga tcgcgggcaa
    15361 cgactcggtg gtggtgctca acatcaagac cgggaaggtc gtgcactcgg ccgccgcggc
    15421 gaccgggacg gaggcccccg gcggggaggc gaccacgacg gtgacgggcc tggagctcgc
    15481 ggcgacggcc tcggtcgtgt ggatcgcgga cttcgacgcc gacagcggcg ggctcggcaa
    15541 caacgcccag gacgaccgcc aggtgcgcaa gttcgagacc ggcgaggccg gcccggtgac
    15601 ggtcgactcg gggctcacca tcgaccccga atcgctgtcg ctcgccaagc gctacggcac
    15661 gacgaaccgg ttctactgga tgaagggcgg gtcgccgttc acggcgctgc tctcggagta
    15721 ggcctggggc gccggcgcgg tcagtcgatc gcgccggtgc cgaccagcgc cagcaccagc
    15781 gcgccgagcg ccacgcggta gaccacgaac acgatcgtcg agttcgtcgc gaggaagcgc
    15841 agcatgaagg cgatcgcgaa gtagcccgag acgaacgcga gcagcgttgc gaccgcggtc
    15901 gggaacgctc ccgccccacc gtcgcccccg atgtccttca gctcgtagag tccgctgagc
    15961 accaccgcgg gcaccgacag caggaacgag tagcgcgcgg cggcggcgcg ctcgaggttg
    16021 aggaacagcc cggcggtgag ggtggcgccc gaccgcgaga cgccggggat cagggcgagc
    16081 gcctgcgcga agccgatggc gacgccgtcg cgggcgccga ggtcgtccag gtcgcgctcg
    16141 cgcttgccga cgcgctcggc cgccagcagc accagcccga gcacgatcag ggtggtgccg
    16201 atcagccgca ggctgcgcgc ctcgtcctcg atctggttgt tgaacacgag tccgaagagc
    16261 gagatcggga tcgtgccgag caggatgtac cagccgaggc gggagtcggt gctcttgcgg
    16321 atgagcgggt cgcgcaggcc ggcgaggaag gcgatcgcga tgcgccagag gtcgttgcgg
    16381 aagtagagga ggacggccgt gagggtgccc agctgggtca cggcggtgaa ggccgcgccc
    16441 gggtcgtccc agccgaggaa cgccgggacg atccggagat gcccggtgct ggagatcggg
    16501 aggaactccg tgagcccctg gacgagaccg aggacgatgg cttcgagcgt ggtcatggcg
    16561 ccggtgcgag agactacgag cgtgacgcgg gcgatcagat acggcgcgcc cggcgtgctg
    16621 gcgttggcgg gcctcgtgct ggtgctcgcc tccgactcgc gcggggccga ctcggccggc
    16681 tacaccctgc tcggctcctc cgcgctgctc gtcctctcga tcgtgatctt ccgggcgggc
    16741 ctcgcaagca acgacgaccg cgaccgcgag gaggccgcgc gcgtgttctt cgacgagcac
    16801 ggccgctggc cgcggcgggg tgagctggac gcgctatcgg ggaaggatcc agggagatga
    16861 ggcagctcac gagccttgac gcggggttcc tggcgatgga gtcgccgcgg acgtacgggc
    16921 acgtcggcgg cttcgccgtg cacgacccga ccacggcgcc gggcgggacg gtcacgcggg
    16981 cggacctgtg ccggctcgtc ggcgagcgca tccacctgct gccgccgttt cgctggaagc
    17041 tcgccccggt gccgctcggg ctcgacaacc cgtactggat cgaggacgcc gacttcgacc
    17101 tcgacttcca cgtgcgcgag accgcggtgc cgccgccggg cggcgcgcgc cagctcggcg
    17161 accagatcgc gcggatcttc gcgcggccgc tcgaccgcac gcgtccgctc tgggagctct
    17221 acctggtcca cgggctcgag ggcgggaagg tcgggatcct cacgaagatc caccacgccg
    17281 ccgtggacgg catgtcgggc gccgagatca tgggcatcct gctcgactcc tcgccggacg
    17341 ggcgcgaggt ggcgccgccc gatcgcgact gcggcgagcg cgcgccgggg cagctcgaga
    17401 tgctgggccg cgggctgctc ggcgtgccgc gccagccgct gcgggcgctg cgggcgcttc
    17461 cctcggccct cccggggctc gacgacgtgc cgacgatccg cacgatccct ggcgtcggcc
    17521 agctcgggcg cgcggccttc caggcgcagt cgtgggcgcg cggctcgcgc gacggcggcg
    17581 tgctggagcg ctcggcggtg cgcgctccgc gcacgcgctt caacgggcgg atctcgccgc
    17641 accggcgctt cgcgttcggg tcgctcccgc tcgacaaggt gaaggcgatc aagaacgagc
    17701 tcggcatcac ggtcaacgac gtggtgatgg cgctctgcgc cacggcgctg cgcgagtggc
    17761 tgatcgagcg cgacgagctg ccgcgcgacc cgctcgtcgc gatggtgccc gtgtccgtcc
    17821 gcacgcccga ggagcagggc acctacggca accgggtctc gacgatgatc gtcccgatcc
    17881 cgaccaacga gcccgacccg cgcgcgcggc tgatgctcat gcacgactcg atgcgcgccg
    17941 cgaaggaccg ccacagcgcg ctgcccgcga gcctgatgca ggacgcgacg cagttcgtcc
    18001 cgccggcgct catgtcgcgc gcggcgcgtg tgatcgcgga gctgtcggag gtcggtccgc
    18061 cgccgctcaa cctcgtgatc tcgaacgtgc cagggccgca ggacccgctc tacctggccg
    18121 gctcgcggct ggtcgcgaac tacccggtgt cggtgatcat ggacggcgtg gggctgaaca
    18181 tcacggtgct gagctacaac ggccggctcg acttcgggat cgtcgccgac cgcgagcaga
    18241 tcgaggacgc ctggccgctc atgcgcaagc tgggggagtc gctcgacgag atcgacgaga
    18301 cgatctgccg ctcgggcaag acggtcagcc cggtcacgca cgggccgcgc agcggttccg
    18361 tcgccgtgag ctgagttgcg cgagagctgg ttccgggcgc tcgtcctcta cccgctgctg
    18421 ttcgtgggac cgtgggtggc gctgagctgg ttgctcgccg agccggagga ggacggcctc
    18481 gtggcggtgc tcggcgtcgg gttctggggg gtcatcgcgg gatcgttcgc gagcctcgtc
    18541 gcggccgtgc tcgtcgcgac gcggcggccg atgcccgggc gcgagcgcgt gctgctcgtg
    18601 gtgctcggcg tgctggtgag cggggcggcg ctcgtgctcg ggatggtcgg ctggttccag
    18661 gcggcgaagt acgactgcgc cgaccagtac gagtgcccga tctgagcctc tagcccggcg
    18721 tgagccgctt cgtgaagtga tgcgaggcga tcacgaggcc gtggcggaag tagaagcggt
    18781 gcgcgtcggc gcggtcgagt ccgaggccgg agtcgaggtg gagctgctcg catccttgcc
    18841 ggcgcgcctc ctcctcgacc caggccatga cggcgtcggc atgtccgcgg ccgcgcaggg
    18901 cggcgcgggt gacgaggtcg tcgacgtaga cgtgacgacc ccaggagagg ctgtggctta
    18961 ggcggaagcc cgcgacggcc gcggcgtcct cgtcgccggg ctcgaacgag gcggcgaggc
    19021 ggtagccctc cgcgcgctgc tcgtcgacgc gccgtgcgat cgcctcggcg ctctcgtatt
    19081 gcggccgcag ctcgagcagc gcggcggcgg cgagcagcga ctcgccggcc gggatctcgc
    19141 gcgcctctgg catccggcgg atcgtaatcg cccgaatctg cggtctctga acagttaacg
    19201 gggtcctagc cccggtaagt gttcaaaacg ccggccactc atcgcgcggc ggccgcgtca
    19261 ttgttcactc accgggcgcc cgcggcttgg cgagggcgcg gtgggggttg gcggggggcc
    19321 ggccgggggg cgctactcga gctcggcggc cgccgcggcg gcggactcgc gcgcctgggc
    19381 ggcttcgtcc cgagcggcgg atgccgcctc gagctcctcg cgcgcggagt ccgcgcgggc
    19441 gcgcgcttcc tcgacctgcc gctcgcgctt gtcgagctcc tccgccgcct tgcgtgtggc
    19501 tcgctcggcc gcctcgacgc ggcgctcggc ctcgctctcc tccttcttcg ccgccttgag
    19561 ccgctcgcgc gcggcccgag cggcggcctc ccgccgctcg cgctccgcag cctcccgctc
    19621 ggcagcttcc gcccgcgcag tctcacgctt gacgcccgcc ttgcccgcgg ccttggaccc
    19681 gccacccttt gtgccgccgt tcccggcgcc ggcggatccc ttggagcggg cggagccggc
    19741 cttcgcggag ccggccttcg cggagccggc cttcgcgagg ccgccaccgc gcgccttgcc
    19801 ggagccgttg ggcgcggcct tgccggagct ctttccgccc ctgctcccct tcgatcgtgc
    19861 gggcgcggca ggtccggcgc ccagggcgaa gcccaggccc gtggcctggc gctcgcggtc
    19921 gagtacgccg gcttcgacca gctcggcgag ctcggggtcg gtggccacgg ccgcgaaggt
    19981 ctcgcggacg cgggtgagcg tcgcctcgcc catcgacttc ccctcgctcg tcaggaggcc
    20041 agacgccgct tccagcaggc cgccgatcgc ctcgcgctgc gcggtggtcg cgcgctcgag
    20101 gtcggcggcg gtcgcctcgc gggtcagcag ccgctcctgc acctcgcgca gtgcggccga
    20161 ggcgtccatc agcgcgcgcc gctccttcgg gcggcagcgg ctcgcctggt tcacggccca
    20221 cgccgccacg ctcggcttcg gcagcttctt gaccgccgcc gcacgctcgc gctcgccctc
    20281 tttgcgcagc tgcttctcga gcgccgtgcg cgccgggatg aactcgtcga gcggcagtcc
    20341 gtagaggtcc gactcctcgc gctccacgac tgagcgttac cccccgcggc gggcggggga
    20401 acgtgccggg cgtcccccct cggtcgaggg gtgacgcccg gacgtgaatt cgttcgcgat
    20461 cagccggagg tcgcggcgct gccggactgc tcgtcctgtg agccgtcctt cgactcggtc
    20521 gcggagccgg cggcgtccgt ggtcgagtcg gccggggccg cagcctccgg agccgggtcg
    20581 gccgccggtg cctcgggggc cggggcctcc gcgggagccg ctgcctcggg ggccgccggg
    20641 gccggagccg cggcctcggg tgccggagcc gcgggggcct ccgcggcggg ggccggagcc
    20701 tcagcggcgg gagccggggc ggcgggcgcc gccggagccg gtgccgccgc agcgggctcc
    20761 gcctcggccg gagcggccgg agcggcctcc gccggagcgg ccgcgggcgc cggagccgcc
    20821 gcagcgggcg ccgcctccgg agctgccgcc tgcgccggag ccgcctcggc cggcgccgcc
    20881 gcgggtgccg gagcagcctg cgccggtgcc gccgcagcgg gcgccgcctc cgccggagcg
    20941 gcctcgggtg ccggcgccgg cgccgtcccc tgctccggag ccgcctgggc ctccgcggtc
    21001 gtgccgccct ccgcgggcgc cgtcgactgg tacgtggcac cgccgctctg cgcgtcgcgc
    21061 gagccgctcg ccttcgacga cgagggctcc tgggacgccg tcgcggcgcg ggtctgcgtc
    21121 cgcgtggtcg acgcggactt cacggtccgc ttggtcgact tgcggaactt catcgcctcg
    21181 aacgggacgt gcaccacgac gaagccggcc cgccgcgagc cgttgaacca gcccgcgcct
    21241 ccgcccgggt tggcccgggt cgtgccgaag aagcggccgt tgatcgacat gtagacgtga
    21301 ccggcgttgg cgtagatcgt cacggcgccg gggcccggcc tgccccaccg catgaagccg
    21361 ccggacacca tcgggctgtt cagcagtccc gccgcgtgca gcacggccga gacggcaccg
    21421 gagcagtcgt acggacccga gaaactcggg ttgtgaccgc cgccccagcg gtacgagtag
    21481 tgcgcgcggt cgatcttgtt cgccgcggcg accatcgccg cgatcatctc ctgcgcgctg
    21541 cccaggccgg gggagacgtt gatcggcccg cccacgggcc ccccaccacc gccgccacca
    21601 ccgccggagg atccgccgcg cgagccggcg ttgccgccgc caccgccgga ggacccaccg
    21661 cgcgagccgc ggtcgaccgt cacgccctcg gtgacgttcg ccgagcgatc gtgcccagag
    21721 ccacgccgct cgcgcttgcg ctgcttgcgt tcgctgcgct cggcgtcgcg gtcggggagg
    21781 atcccgccgt cggcctcggc gctctcgggc gctccgccgt cacgcgcctg gttgcgcgcg
    21841 gtgcgcaggt cctggtcgct ctcggccggt ccgttggccg cgagccccgt gggccctccg
    21901 accgaggccg ccgagaccgg cgcggccggg ttcgccccgg cgagcccctg cacggcctgc
    21961 ttcggcgcct gcgcagcgag ctgcgcgctc tggttcgacg ggtcgtagac gaccatgccg
    22021 tcggggaaca gcgtcagcca cgtgcgcacg tcgaccttgc cggtcggcgg gagcccgtgc
    22081 ttcgcctggt agcgcttgac ggcacgctcc gtgatcggcc cgaagatgcc gtcctcggcc
    22141 accttcagct cttgctgcac ctcgacgacc gcggcgctcg acatgccgcg ctcgagcagg
    22201 atccgggtcc cgtcgtcgcg gtcctcgagg atccgcgagt ggctggcgga ggcccgctgg
    22261 gggccgtcgc cgccgaaggt cttgaaggtc ggggcgatag gggtagttgc catcgcggcg
    22321 gtgaccgcca gcgacgcgct cttctggcgg gactggtgct tgcgtgcgag ctcactgagc
    22381 tcacggcggc gctgcgaacg ctcgagcgac tcatgccaaa gctgagtcga ggacagatct
    22441 cggggcgaag gggccaacga cgtctccttc caattcgggt aacgcctacg aggtgagctg
    22501 acgggctcgc gcgtggaatc gcgctacgcc gcgcaccggc ggcgattcgc ccctccgacc
    22561 attgcggccg gtttggttcc cccgctcccg ggcttcatgg aagaccggga ctcggcgtct
    22621 atgtcaaacg aaaaatacct gctcgttgca agaactttcg cagcgcagaa ccttaacggg
    22681 cctgtcaacc gggcggacgg cgaacaatcg gtttgcgcgc caccacccgt ggaatacgcc
    22741 ggatgacggg ctggcggggg agtccgttct ttaggggggc agcaagcgcg cgcctcccgg
    22801 cttcacacaa gccgggaggc gtcgcgcgtt tggcgccctg acggtgttgg tacccctcga
    22861 aatgcacgcc atgcgcaggt cgctcctaac catcctcgcg ctcgcgctga ccctcgcggc
    22921 gcccgcgagc gcgcaggagc gcgacctcgc gtcctacgcc gacccgatgg tgggcacggg
    22981 cccgcccggg ttcgtcttcc cgggggccgt ggtcccgttt gggatggtcc agaactcgcc
    23041 ggacacccgc ggcgagttcg cctacagcgg ctacctctac gcggacccgc agatccaggg
    23101 cttcagcctc gtgcacatat ccggcccggg cgtgcgcaag ggcggcgaca tcccgctgat
    23161 gccgacggtg gggccggtca actcgaacga cccggtccag tacggctcgc cgttcgacca
    23221 cgcgcgggag gaggccgagc ccggctacta ccgcgtgcac ctcgcgaagt acgccaccga
    23281 cgtcgagctg acggcgtcca cccgcgccgc catgcagcgc tacacgttcc cgccctcgcc
    23341 gcagtcgaac gtgatcgtcg acgtgggacg cagcgtcgag ggcgtgcact ccgggcagat
    23401 ccgcttcacc ggccccgacg agatcagcgg ctacagcgag gggcgctacc gcgtctactt
    23461 cgtggcgaag ttctcgcggc cgttcgcgtc gaccggcacg ttcaagcccg acggcgaggg
    23521 cgcgggcggc tgggccacgt tcaacacgct caccgaccgc caggtcaccg cccgcatcgg
    23581 catctcgttc gtcgacgcgg cgggcgcgcg gcgcaacctc gaagccgacg cgcccacgtt
    23641 cgacttcgac cgcatgcgcg cgagggcgcg cgaggcctgg aaccgcgagc tcggccggct
    23701 gaacgtgagc ggcgggaccg acctcgagcg cacgaccttc tacagcgcgc tgtaccactc
    23761 gtcgctgcac ccgaacgtct tcagcgacgt ggacggccgc tacatgggct tcgacaagca
    23821 gccgcacagg gccgagggcc gcacccagta cgcgaacttc tcctcctggg acctctacaa
    23881 gagcaccaac cagctcctcg cgacgctcca gccgcgccgc taccgcgaca tgctcctgac
    23941 gctgctcgcg aactaccgcg agtgggggcg gctgccgcgc tggggcgagc actacttcga
    24001 cgccaaccac atgtccggcg acccggccat cccgatgatc gccgacggtt tgtgccgcgg
    24061 cggaatcctc agccacggcg aggccgagga cctgctcgag gcctcgaacg cgctctaccg
    24121 ccggcgcgag gcgagcctcc agcagctcgg ctacctgccg gaccgcccgg ggaccacgct
    24181 cgagttcggt gtcgccgact tcgcgctggc gctgctcgcg gacgccgtcg gccacgactc
    24241 cgaggccaag gcgcggctga ccgactcgct gcgctaccgc accgtgctcg accccgagac
    24301 ccgctgggtg cgcccgcgac acgccgacgg aagctggtac gagcccttcg atcccacgga
    24361 cacgacgggc ttccaggagg gcaactcgtg gcagtactcg tggctcgccc cgcacgacgc
    24421 gcgcggcctc tacgaccgca tgggcggcga cgcggccgcg atcgagcgcc tcgaccagta
    24481 cttctcggcc ccgcccgagg cggccaatca gctcacgttc ttcgggatcg tctacaaggc
    24541 gccctggcac gcgcccggca acgagcacga cctccaggtg ccgtggatgt acccgttcgt
    24601 gggccagccg tggaagacga tgtccgagca ccgcgagatc cagcggctct tccggcccga
    24661 gccgaacggc ctgcccggca acgacgacct cggcgggctc tcctcgtggc tcacgtggtc
    24721 gatgctcggc atcggtccgg tcacgccagg cgcgccgttc tacgtgatcg gcagccccac
    24781 cttcgagcgc gccgtgatca cgcccgacgg cggcagccgg ttcgcgatcg aggcaccggg
    24841 cgcgtccccg ctgcgcccgt acgtgaccgc ggcgagcgtc ggcgggcgcc ggctcggcag
    24901 tgcctggctc tacgacgcct cggtccgccg cggtgagacg gtgaagctga ccgtcgccga
    24961 gcagccgaac acggagttcg gctccgcgcc gccggtccgg ccgccctcgg tgagcgactc
    25021 gccgctcgcg cgcttcggct gcctgcccac ggcgccccag cccgacccgg tggcgcccgg
    25081 cgcggccgag cgcccggccg gcttcgagga tccccgcacg gcgccggtgg agccgggacc
    25141 ggtgctggcc gacggcagcg cgccgcccag cctcgtcccg ctgcggctgc gcgtctcgcc
    25201 gcgcacggtg cgcacctcgc ggcgcacgct ctaccggctc accgtccacg cgaagcgcca
    25261 tggccgctgg gtgccggtgc gcggcgcgcg ggtgcgcctg gcgggagtcc gggcgcgcac
    25321 gtcgggcaag ggcgtggcca agatccgggc cacgatccgc caccacggca cccaccgcgc
    25381 caacgcgacc gccgccgcgg cgcgcccggc gtaccgctcg
//
LOCUS       DASVPL010000286         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_33877, whole genome shotgun sequence.
ACCESSION   DASVPL010000286 DASVPL010000000
VERSION     DASVPL010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33877"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..387)
                     /locus_tag="VF517_16020"
     CDS             complement(16..387)
                     /locus_tag="VF517_16020"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8054495.1"
                     /translation="MSARQRVSGIGGLFFRAHDPERLRRWYEEYLGVVAPPSNHVEEP
                     WEQQAGPTVFAPFRQETEYFGRPEQGWMVNFRVEDLDAFVAQLRAEDVAVEVDPEEYP
                     HGRFARLHDPEGNPVELWEAV"
     gene            complement(384..>2376)
                     /gene="recG"
                     /locus_tag="VF517_16025"
     CDS             complement(384..>2376)
                     /gene="recG"
                     /locus_tag="VF517_16025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX8054496.1"
                     /translation="ESLGLANVGDLLEHVPFRHEDRRDARPVASLTPGEEATVIVDVK
                     RISNRRTRYGKTLQEATVTDETGPMKAVWFNQPWLADKLQPGTRVVLRGKAGSGKKGF
                     SVSSYEIAPGASALQVKGEGMTPIYSTTEGMSSDRLRELAKAARGAERDTVETLPAWM
                     RVAEGLPERAAALSAVHFPEDEQETEHARRRLAFEELLALELAFVARKRARELTVRAA
                     ELEPTGDLVGPWLASLPFAPTGDQRRAMEEVDRDVRSGRPMQRLLMGEVGSGKTVVAL
                     HAMLRAVETGRQAALMAPTETLAEQHLATLDRLLGGHVPIELLTGSTTAARRRDLLAR
                     LETGELGLVVGTHALIEPTVRFRDLALVVVDEQHRFGVRQRAALEAKAPGELVPHVLH
                     LTATPIPRTLQLTAYGDLDVTILKELPAGRRPVETFVGDTASWRARAYERAREEIAAG
                     RQVFVVCPLVEESEALQAAAATVEAERLRTTEFSRHSVELIHGQMPSKQKSEAMRAFA
                     ERKADVLVATSVIEVGIDVPNATVMVIEAAGRYGISQLHQLRGRVGRGEHAGLCILFG
                     DPKQPRLAALAEERDGFRLAEVDLEIRGAGDLLGTRQHGLPTLRVARLPADADLLERT
                     RERAFEALDADPGLTGPEHALMRAAATARFGGEREPIPA"
BASE COUNT          293 a          883 c          874 g          326 t
ORIGIN      
        1 ccacgcgcat cggcgctaga ccgcctccca gagctcgacc gggttgccct ccgggtcgtg
       61 caggcgagcg aagcgcccgt gcgggtactc ctcgggatcg acctccaccg ccacgtcctc
      121 ggcgcggagc tgcgccacga aggcatcgag gtcctcgacc cggaagttga ccatccaccc
      181 ctgctccggg cggccgaagt actccgtctc ctggcggaac ggcgcgaaga ccgtcggccc
      241 ggcctgctgc tcccagggct cctcgacgtg gttgctcgga ggcgcgacaa cgccgaggta
      301 ctcctcgtac cagcgcctca ggcgctcggg gtcgtgcgcc cggaagaaca ggccgccgat
      361 gccgctgacg cgctggcgcg cgctcacgcc gggatcggct cgcgctcgcc gccgaagcgc
      421 gccgtggcgg ccgcccgcat cagcgcgtgc tcggggcccg tgaggccggg gtcggcgtcg
      481 agcgcctcga acgcgcgctc gcgggtgcgc tcgagcaggt cggcgtcggc ggggaggcgc
      541 gccacgcgga gagtgggcag cccgtgctgg cgggtgccga gcaggtcgcc ggcgccgcgg
      601 atctcgaggt ccacctcggc gagccggaag ccgtcgcgct cctcggcgag cgcggccaga
      661 cggggctgct tcgggtcgcc gaacaggatg cagagcccgg cgtgctcacc gcgcccgacg
      721 cggccgcgga gctggtgtag ctgcgagatc ccgtagcggc cggccgcctc gatcaccatg
      781 acggtcgcgt tcggcacgtc gattccgacc tcgatcacgc tggtggcgac cagcacgtcg
      841 gccttgcgct cggcgaacgc gcgcatcgcc tcggacttct gcttcgatgg catctggccg
      901 tggatcagct cgacgctgtg gcggctgaac tcggtcgtcc gcaggcgctc ggcctcgacg
      961 gtcgccgcgg cggcctggag cgcctcggac tcctccacga gcgggcagac cacgaacacc
     1021 tggcgcccgg cggcgatctc ctcgcgcgcg cgctcgtagg cgcgagcccg ccacgaggcg
     1081 gtgtccccca cgaacgtctc gaccggccgc cggcccgcgg gcagctcctt caggatcgtc
     1141 acgtcgaggt cgccgtaggc ggtgagctgg agcgtgcgcg ggatcggcgt ggcggtgagg
     1201 tggagcacgt gcgggaccag ctcgccgggc gccttcgcct cgagcgccgc ccgctggcgg
     1261 acgccgaagc ggtgctgctc gtccaccacg accagcgcga ggtcgcggaa gcgcacggtc
     1321 ggctcgatca gcgcgtgcgt gcccacgacc aggccgagct cgccggtctc gagccgcgcg
     1381 agcaggtcgc gccggcgcgc ggccgtggtc gatccggtca gcagctcgat cggcacgtgg
     1441 ccgccgagca ggcggtcgag cgtggcgagg tgctgctcgg cgagcgtctc ggtgggcgcc
     1501 atgagcgcgg cctggcgacc ggtctcgacg gcgcgcagca tcgcgtggag cgccacgacg
     1561 gtcttgccgg agccgacctc gcccatcagc agccgctgca tcggccgccc cgagcgcacg
     1621 tcgcggtcga cctcctccat cgcccgcctc tgatcgcccg tcggggcgaa cgggagcgag
     1681 gccagccacg gaccgaccag atcgccggtg ggctcgagct cggcggcccg cacggtgagc
     1741 tcgcgggcgc gcttgcgggc gacgaacgcg agctcgagcg cgagcagctc ctcgaacgcg
     1801 agccggcggc gggcgtgctc ggtctcctgc tcgtcctccg ggaagtgcac ggccgacagc
     1861 gcggcggcgc gctcgggcag gccctcggcc acgcgcatcc aggcgggcag cgtctcgacc
     1921 gtgtcgcgct cggcgccgcg cgcggccttg gccagctcgc gcaaccggtc cgaggacatc
     1981 ccctcggttg tcgagtagat cggggtcatg ccctccccct tgacctggag tgcgctggcg
     2041 ccgggagcga tctcgtaact gctcacggag aagcccttct taccgctgcc ggccttgccg
     2101 cggagcacca cgcgggtacc cggctgcagc ttgtccgcca gccacggctg gttgaaccaa
     2161 accgccttca tcggcccggt ctcgtcggtg acggtcgcct cctgcaacgt cttcccgtag
     2221 cgggtccggc ggttgctgat ccgcttcacg tccacgatca ccgtggcctc ctcgccaggt
     2281 gtgaggctcg cgaccggtcg cgcgtcgcgg cggtcctcgt ggcggaacgg cacgtgctcg
     2341 agcaggtcgc cgacgttggc gagcccgagc gactcg
//
LOCUS       DASVPL010000287        13219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_113322, whole genome shotgun sequence.
ACCESSION   DASVPL010000287 DASVPL010000000
VERSION     DASVPL010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13219
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113322"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..2883
                     /locus_tag="VF517_16030"
     CDS             109..2883
                     /locus_tag="VF517_16030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX8054497.1"
                     /translation="MTLSRTHTQTDFAPLEVERFEHVPGGRELALLRVEGRYRSKLAR
                     PLLEAALLVDDGLAIHRHDPLPESFDMREGLGDDDWLWRSAFAVSCAALEDEDTTFSI
                     QVAPNLVLEVGRPATWAAPRATRRRSRPAAATSRRAVAASILLALSLTPVNINGLADA
                     AVLKLKKADGTMEAIVTQTPPLDHPPYNDLCAQPENAGACDQAPQTVNSQPAPAPAPA
                     EPAQQEPKDPAPSGSEEKPDKAPSGDRGRSQDGDHGDEAKKPKKPKKHHSSSGRSGGR
                     SAGHRNSHPSRRSTPRKPVRHRDGSPTRSAPGYFDALPTPEKAEGVPNFVIRKFRVPV
                     FLLPIYQAAGTEYGIRWEVLAAINEIETDYGRNLNISSAGALGWMQFMPPTWKSYGVD
                     ANKDGKKDPFNPVDAIFAAARYLNASGGEKDIRRAIFAYNHADWYVDSVMLRAKLLSG
                     VPTDVVGSLTGLTEGRFPVAARARYADDIDAQNATHRLKRGPNANNVVHDNPARDGIE
                     IFSERGAPVVAVNDGEVERIGHTRKHGNYVVLRDVYGNEYLYAGLGNVADFYPVPKKN
                     PGPSGNEAKAVSARTDAGRPQPKVTSKRRVFAHPSRARSKTAGGLEQIFDAEARDGGK
                     FQTYGHVFTPGLGLDRDNATLRRLKPGARVIGGTVLGRVGRPDPKKAAHMNFMVRPAG
                     RGAPRIDPKPILDGWKLLESTAIYRAKGKNVLKDSSSASIGQILMMAKPLLERRVLND
                     ERIELDASGRNDIRTGQIDRRVLAVLAYLAESGLKPTVSSLRSDHGTYTSSGNVSEHS
                     TGTAVDIAAINGIPIVGHQERGGITHQAVKRLMALQGTMRPHQIISLLDLGQNTVAMG
                     DHNDHIHVGFRPNAGDNRKTGRAAMAVLDPDQWTDLMKRLGEIENPVVPTEPSRFALP
                     VKPKHRGHGD"
     gene            2902..5052
                     /locus_tag="VF517_16035"
     CDS             2902..5052
                     /locus_tag="VF517_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8054498.1"
                     /translation="MDATVKQKRRWLIEFSLVSLIPMILLGYFLAQTLGDQARERSVA
                     SARSEARLVSDIAVRRALGSEIEASRALTREGQDALGRAVVSARAGTDVARITVRDRT
                     GTVLWSDDRLKIGESDKPPTEARDALYGNTVSKVVDMSKNPITQDASLGRVLKIYVPL
                     RAEPGGPPVGSSEVWIPYAGIASQIEKDTRTLYVAIGVGLLLVWGCIVGVVAGASQRL
                     RRQAAEKEEQALSDGLTGLPNRTMFASLLEKTISATGRRKKLGVVMLMDLDRFKDVND
                     TLGHHNGDLLLQRIGSRLHSVLRESETVARLGGDEFAILLPDVPDRQSVVPVVRRVLK
                     VLEEPVVVGGLALQCEGSIGIAIFPEHGKTVDSVMRAADVAMYMAKENRSGYEFYDAR
                     RHEHRHDAGRLALIGELRRAMDETELVLYYQPKVNMRTGRAEGVEALARWHHPERGLL
                     SPDEFIPLAERSNLLRPMTLYLLDSALRQANAWRTRGLEVSVSVNLSMQNLIDMRLPN
                     DLARLLTSWRLPPGSLELEITESTIMADHRRAMTILTRLNKMGVALSVDDFGTGYSSL
                     AYLQNLPVDSIKIDKSFCISMSEDAGNATIVQSTIDLGHNLGLQVVAEGVETIEAYNK
                     LRELGCDYAQGYFLSRPLAPEKATIWLETMMAGEEPREIEPAEPVPVDEDLDALSEWS
                     LEAPASALALQAEAEAEAEADVESDTELDAETAS"
     gene            complement(5049..7889)
                     /locus_tag="VF517_16040"
     CDS             complement(5049..7889)
                     /locus_tag="VF517_16040"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8054499.1"
                     /translation="MLLERESELRDLEREIGEACAGRGRVVLVEGEPGIGKTRLLEVV
                     RERSREHGMTVLSARASELDRDFPLGVVRQLFEPLLAGADAATRRALLHGAAAAAAPL
                     LGLAAPEPGGAPAADDPSLPHFHALYWLAANLTERGPAALVIDDLHWADASSLRFLQF
                     LLPRLDELPVLVALAARPDEAGSDRRSIDALATDARAVVLRPPPLSEDAVPALVAARL
                     GPDPDRAFCDACRAATGGNPFLLRELLRELAADGVAPAAAAAPLVRQLAPPTVGRAVL
                     LRVARLGPDAAALARAVAVLGDGAPLRRAAALAELGEDLAAAAAEHLAGAGILAAARP
                     LAFAHPILRSAVYADIAAGERADAHRHAAEVLAAEGAGADAVAVHLLATEPAAEPRVV
                     ATLREAARDALARGAAPAAAACLRRALAEPPATDDRAALTLELASAELHAGEPVAAAE
                     HFEEGARLAGDPRVRASCAVEWSAALQAAGRHQESYAVRERAIEDVADADPELALLLE
                     TTLIATASLDLARLEWARARLRRHDAAAEPATPAGWRLIATQAYVDAMYGDSPAATLA
                     AAAERALGAREPAGAPSGFAGTPFFTAVEVLALADANGPAREALDQAVADARRQGSAL
                     AFACSSGWRCMLLARLGELAEAEADARSCAELSLAQGWFALAPPMLGYVLDVLIARGE
                     LDDAERILIEAGAAERAAGDDLTFYPVIHARARLRAARGDTGGAHRDMTELARRRARW
                     NTDLTLVPPLLAAPELAHDDADAARSETAAMLGEARAWGTPRAIGMALRASGLAEGGT
                     RGVELLEEAAAVLGESPARLEHARALADLGAALRRANRRSAAREPLRRALDLADACGA
                     GPLAERARQELRAAGGRPRRPRISGVGALTASERRIAAMAAEGLSNPEIAQALFVTKK
                     TVETHLGNAYRKLGIRSRGALGAALGGSERED"
     gene            8025..8219
                     /locus_tag="VF517_16045"
     CDS             8025..8219
                     /locus_tag="VF517_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054500.1"
                     /translation="MTNAEQPRRLALEVRFDAEPIEGRVSEGDGTARLDRPFSGWLGL
                     MAAIDAARGTDAREQRGEQR"
     gene            8216..9802
                     /locus_tag="VF517_16050"
     CDS             8216..9802
                     /locus_tag="VF517_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054501.1"
                     /translation="MRRRNLWLTVGLAAAAVGLAPGSAGAAGFAETAGCDEQQAFVEG
                     DEAAVRARLPERYAPVRTPAGRPLLFVRALRCDAVTLGERSRQAVMASYGVVVESPDG
                     EGCASDAPVAGTVKGDEPPVCNWYTLAWLSNDRRVVDWLREGTPSFPAFHVEQLAFDL
                     TAFGAGRAGAAFSFSAASPSPSPFAMEATTRPRPGPLSVRGGYWFDTPQGTVKVALST
                     DDLTSGDATGVARAGRGSELAALLGAEERPYVPGYSAFAAERWDHASYRKQLYRAPAG
                     PRAGAGDGFAGSCSVQGTVTFKPPASYYRQPLDYAYESAGTCSGTLNGREIADVPVKL
                     RQAGRSEGSCASARTISPGTGSIVFAGGELIRYTLDFSSTGTEIDFTLYGERSGQASG
                     KGSFLTQRTPPDVTARCASEGASEVPMDMTLTTESQLVSEPPARTHPGTPVRPQLRLA
                     VSPRAVRAGRGMRYAFRVTAAGRPVRGALVRFAGRAIRSDAAGRAALRVRLHRPGPHP
                     ARAVKPGFLSARSAVTVTRR"
     gene            complement(9799..10707)
                     /gene="uvsE"
                     /locus_tag="VF517_16055"
     CDS             complement(9799..10707)
                     /gene="uvsE"
                     /locus_tag="VF517_16055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEX8054502.1"
                     /translation="MKILGAGGMRTSDTRRWQSGPHLSRSIELLDGAFDHLHANGVSV
                     FRLSSSTVPYGTHPDLPQLDYRRQIDDCAAALAELGAKARAYGLRLSTHPGQYSVLNS
                     QSEDVAAKARLDLEQDTALLDALGTGPEAVVVIHVGALGGDRTAALDRFARSFELLSE
                     RARARLAVEHDERLFDVADVLELHRRLGCTVIYDHHHHRCNVAPGYEDPARAIEEVAA
                     TWPAGVRPKLHLSSPRTELRAGGRAPLLDQHADFVTPFDLREVLDAAARPVDVMIEAK
                     AKDLAVAWLRTQLERLWPGYAAAEER"
     gene            complement(10779..11243)
                     /locus_tag="VF517_16060"
     CDS             complement(10779..11243)
                     /locus_tag="VF517_16060"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8054503.1"
                     /translation="MSTIEESIDLEVPVRNAYNQWTQFEEFPQFMEGIEAVEQLDDTH
                     LHWTAEIAGQRREWRAEVTEQTPDQRIAWRSIDGTQHAGVVTFHRLDDGQSRIMLQLD
                     AEPEGVVEKAGDALGLLKRRVKADLERFKELIESRGAESGAWRGEVAQDPAA"
     gene            11325..12425
                     /locus_tag="VF517_16065"
     CDS             11325..12425
                     /locus_tag="VF517_16065"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX8054504.1"
                     /translation="MAGRIVLFGATGYTGRLTAEALAARGARPLLAARNEERLRSLAD
                     ELGGGFDVAVADVARPDSVRDLVERGDVLVSTVGPFARWGDPAVEAAIATGAHYIDSN
                     GEPAFIRRVFERFGPGAEAAGCGLVTAFGYDWVPGNLAGALALREAGDAAVRVAIGYY
                     AIGRSQFSGGTQASAAGALAEPAFAFRGGMLQTERGAKRVANFMVEGERRPGISVGSS
                     EHFGLPRVAPGLREVDVYLGWFGPMSRPMQVGAAVTAAATRIPGVKTGLNALTARVSK
                     GSTGGPGAEERGESRSYVVAQALDAGGTCLAEVRIEGVNGYDFTARMIAWGAERAAAS
                     GLEGTGALGPVDGFGLDELERGAAEAGLARAG"
     gene            complement(12422..13114)
                     /gene="rpiA"
                     /locus_tag="VF517_16070"
     CDS             complement(12422..13114)
                     /gene="rpiA"
                     /locus_tag="VF517_16070"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX8054505.1"
                     /translation="MSTDAAREAAARAAVERVEPGMTLGLGSGRAVWRTIELIAERWP
                     DGPPVRSAFASDRTAALAAEHGIEPVELDGATGLDLAIDGADEIDPQLALIKGGGAAL
                     LREKIVVEAARRFVVVAEERKRVSRLGESFRLPVEVVRFGWPDTRRRVLDLLPAAELR
                     TDDSGAPVVTDEGHHLLDCEIGPGTDPGDLGRALKAVTGVVEHGLFVGLADEALLGRD
                     DGSLEVARPQAG"
BASE COUNT         1763 a         4946 c         4893 g         1617 t
ORIGIN      
        1 gaccgtcacc gcgctcccag cctggcgggt ggcgggtccg ccattccggc gcgtcggctc
       61 cccttaaggc tccacgagcg actgccgaca aagctcaaac agtcgtccat gaccctctcc
      121 cggacccaca cccagaccga cttcgccccg ctcgaggtgg agcgcttcga gcacgtcccc
      181 ggcgggcgcg agctcgcgct gctgcgtgtc gagggtcgct accgctcgaa gctcgcccgc
      241 ccgctgctcg aggccgcgct gctggtcgac gacggcctcg cgatccaccg tcacgacccc
      301 cttccggagt cgttcgacat gcgcgagggc ctcggcgacg acgactggct ctggcgctcc
      361 gccttcgccg tctcgtgcgc cgcgctcgag gacgaggaca caaccttctc catccaggtc
      421 gctccgaacc tcgtcctgga ggtcggtcgc cccgccacgt gggcggcacc gcgcgccacc
      481 cggcgccgct cgcgcccggc cgccgccacg agccgccgcg cagtggccgc ctcgatcctg
      541 ctggcgctct cgctgacccc cgtcaacatc aacggcctcg ccgacgcggc cgtgctcaag
      601 ctcaagaagg ccgacggcac gatggaggcg atcgtcaccc agaccccgcc gctcgatcac
      661 ccgccgtaca acgacctctg cgcccagccc gagaacgcgg gtgcctgcga ccaggcgccg
      721 cagaccgtca actcgcagcc cgcgccggcg cccgccccgg ccgagcccgc gcagcaggag
      781 cccaaggacc cggccccgtc cggcagcgag gagaagcccg acaaggcccc ctccggcgac
      841 cgcggccgca gccaggacgg cgaccacggc gacgaggcga agaagcccaa gaagcccaag
      901 aagcaccaca gctcctccgg ccgctccggt ggccgcagcg ccgggcatcg caacagccac
      961 ccgagccgca gaagcacccc gcgcaagccg gtccgccacc gcgacggcag cccgacccgc
     1021 tcggcccccg gctacttcga cgccctcccg actcccgaga aggccgaggg cgtccccaac
     1081 ttcgtgatcc gcaagttccg cgtgccggtc ttcctgctcc cgatctacca ggccgccggc
     1141 accgagtacg gcatccgctg ggaggtcctc gcggcgatca acgagatcga gaccgactac
     1201 ggccgcaacc tcaacatctc ctccgccgga gcgctcggct ggatgcagtt catgccgccg
     1261 acgtggaagt cctacggcgt cgacgcgaac aaggacggca agaaggaccc cttcaacccg
     1321 gtcgacgcca tcttcgccgc ggcccgctac ctgaacgcca gcggtggcga gaaggacatc
     1381 cgccgcgcga tcttcgccta caaccacgcc gactggtacg tcgactcggt gatgctgcgc
     1441 gcgaagctgc tgtcgggcgt tccgaccgac gtggtcggct cgctcacggg cctgaccgag
     1501 ggccgcttcc cggtcgcggc ccgcgctcgc tacgccgacg acatcgacgc gcagaacgcg
     1561 acccaccgcc tcaagcgggg ccccaacgcc aacaacgtcg tgcacgacaa cccggctcgc
     1621 gacggcatcg agatcttctc cgagcgcggc gcccccgtgg tcgcggtcaa cgacggcgag
     1681 gtcgagcgca tcggccacac ccgcaagcac ggcaactacg tggtcctgcg cgacgtctac
     1741 ggcaacgagt acctctacgc cggcctcggc aacgtcgccg acttctaccc ggtgcccaag
     1801 aagaaccccg gcccctcggg caacgaggcc aaggccgtct cggcgcgcac cgacgcaggc
     1861 cgcccgcagc cgaaggtcac ctcgaagcgc cgcgtgttcg cccacccgag ccgcgcccgc
     1921 tcgaagacgg ccggcggcct cgagcagatc ttcgacgccg aggcgcgcga cggcggcaag
     1981 ttccagacct acggccacgt cttcaccccg ggcctcgggc tcgaccgcga caacgccacg
     2041 ctgcgccgcc tgaagcccgg cgcgcgcgtg atcggcggca cggtgctcgg ccgcgtcggc
     2101 cgcccggacc cgaagaaggc cgcccacatg aacttcatgg tccgcccggc cggcaggggc
     2161 gcgccgcgga tcgacccgaa gccgatcctc gacggctgga agctgctcga gtcgacggcg
     2221 atctaccgcg ccaagggcaa gaacgtcctc aaggacagct cgagcgcgtc gatcggccag
     2281 atcctgatga tggccaagcc gctgctcgag cgccgggtgc tgaacgacga gcggatcgag
     2341 ctcgacgcga gtggccgcaa cgacatccgc acgggtcaga tcgaccgccg cgtgctcgcc
     2401 gtcctcgcgt acctcgccga gtccggcctg aagcccacgg tcagctcgct gcgctcggac
     2461 cacggcacct acacgagctc gggcaacgtc tcggagcact cgacggggac cgccgtcgac
     2521 atcgccgcga tcaacggcat cccgatcgtc ggccaccagg agcgcggcgg gatcacgcac
     2581 caggcggtca agcgtctgat ggccctccag gggaccatgc gcccgcacca gatcatctcg
     2641 ctgctcgacc tcggccagaa caccgtggcg atgggcgacc acaacgacca catccacgtc
     2701 ggctttcgcc cgaacgccgg tgacaatcgc aagacgggcc gcgccgccat ggccgtcctc
     2761 gaccccgatc agtggaccga cctgatgaag cgcctcggcg agatcgagaa cccggtcgtg
     2821 ccgaccgagc cctcgcgctt cgccctgccg gtcaagccca agcaccgcgg gcacggcgac
     2881 tagcccgcat agaggcccct cgtggacgcg accgtcaagc agaagcggcg ctggctgatc
     2941 gagttcagcc tcgtcagcct gatcccgatg atcctgctcg ggtacttcct ggcgcagacg
     3001 ctcggtgacc aggcccgcga gcgcagcgtc gcctcggccc gctccgaggc ccgcctcgtc
     3061 tccgacatcg ccgtgcggcg cgcgctcggc agcgaaatcg aagcctcacg ggcgctcacc
     3121 cgcgagggcc aggacgcgct cggccgcgcg gtcgtctcgg ctcgcgccgg gaccgacgtg
     3181 gcccggatca ccgtgcgcga tcgcacaggc accgtcctct ggtccgacga ccgcctgaag
     3241 atcggcgagt ccgacaagcc gccgaccgag gcgcgcgacg ccctctacgg caacacggtc
     3301 tcgaaggtcg tcgacatgag caagaacccg atcacccagg acgcgagcct cggccgcgtg
     3361 ctgaagatct acgttccgct gcgcgccgag ccgggcggac cgccggtcgg ctcctccgag
     3421 gtgtggatcc cgtacgcggg catcgccagc cagatcgaga aggacacgcg cacgctctac
     3481 gtggcgatcg gcgtgggcct cctgctcgtg tggggctgca tcgtcggcgt ggtcgccggc
     3541 gcgtcgcagc gcctgcgccg ccaggccgcc gagaaggagg agcaggcgct ctccgacggc
     3601 ctcaccggcc tcccgaaccg cacgatgttc gccagcctgc tcgagaagac gatctccgcc
     3661 accggccgcc gcaagaagct gggcgtggtg atgctgatgg acctcgaccg tttcaaggac
     3721 gtcaacgaca cgctcggcca ccacaacggc gacctgctgc tccagcggat cggctcgcgg
     3781 ctgcacagcg tgctgcgcga gagcgagacg gtcgcccgcc tcggcggcga cgagttcgcg
     3841 atcctgcttc ccgacgtgcc cgaccgccag tcggtcgtgc cggtggtccg ccgcgtgctc
     3901 aaggtgctcg aggagccggt cgtcgtgggc ggcctcgcgc tccagtgcga gggctcgatc
     3961 ggcatcgcga tcttccccga gcacggcaag accgtggaca gcgtcatgcg cgccgccgac
     4021 gtggcgatgt acatggccaa ggagaaccgc tcgggctacg agttctacga cgcgcgccgc
     4081 cacgagcacc gccacgacgc gggccggctc gcgctgatcg gcgagctgcg ccgcgcgatg
     4141 gacgagaccg agctggtcct ctactaccag ccgaaggtga acatgcgcac gggtcgcgcg
     4201 gagggcgtcg aggcgctcgc gcgctggcac caccccgagc gcggcctgct ctcgccggac
     4261 gagttcatcc cgctcgcgga gcgctcgaac ctgctgcgcc cgatgacgct ctacctgctc
     4321 gactccgccc tgcgccaggc gaacgcgtgg cgcacccgcg gcctagaggt gagcgtgtcg
     4381 gtgaacctgt cgatgcagaa cctgatcgac atgcggctgc cgaacgacct cgcgcgcctg
     4441 ctcacgagct ggcggcttcc cccgggctcg ctcgagctgg agatcaccga gtcgacgatc
     4501 atggccgacc accgccgcgc gatgacgatc ctcacgcgcc tcaacaagat gggcgtggcg
     4561 ctctcggtgg acgacttcgg caccggctac tcgtcgctcg catacctcca gaacctgccg
     4621 gtggactcga tcaagatcga caagtccttc tgcatctcga tgagcgagga cgcgggcaac
     4681 gcgaccatcg tgcagtcgac gatcgacctc ggtcacaacc tcgggctcca ggtcgtagcc
     4741 gagggcgtgg agacgatcga ggcctacaac aagctgcgcg agctcggctg cgactacgcg
     4801 cagggctact tcctgagccg cccgctcgcg ccggagaagg ccaccatctg gctcgagacg
     4861 atgatggccg gcgaggagcc gcgcgagatc gagccggccg agccggtgcc ggtcgacgag
     4921 gacctcgacg cgctcagcga gtggtcgctc gaggcgccgg cgtccgcgct cgcgctccag
     4981 gccgaggctg aggccgaggc cgaggccgac gtcgagtccg acaccgagct cgacgccgag
     5041 accgcgtcct agtcctcccg ctccgagccg ccgagcgcgg cgccgagcgc tccgcgggag
     5101 cggatgccga gcttgcggta cgcgttgccg aggtgggtct cgacggtctt cttcgtaacg
     5161 aacagcgcct gcgcgatctc ggggttcgac agcccctccg cggccatcgc ggcgatgcgg
     5221 cgctcgctcg cggtgagcgc gccgacccct gagatgcgcg gccggcgcgg gcgcccgccc
     5281 gccgcgcgca gctcctgacg cgcgcgctcg gccagcggcc cggcaccgca cgcgtcggcg
     5341 aggtcgagcg cccggcgcag cggctcgcgc gcggccgagc gccggttcgc gcgccgcagg
     5401 gcggcgccca ggtccgccag cgcccgcgcg tgctcgaggc gcgccggcga ttccccgagc
     5461 acggcggccg cctcctccag cagctcgacg ccgcgcgttc cgccctcggc cagcccggac
     5521 gcgcgcagcg ccatgccgat cgcgcgcggg gtgccccagg cgcgcgcctc gcccagcatc
     5581 gccgcggtct ccgaccgggc tgcatccgcg tcgtcgtggg ccagctccgg agccgccagc
     5641 agcggcggca cgagcgtcag gtcggtgttc cagcgcgcgc gccggcgggc cagctccgtc
     5701 atgtcgcggt gggcgccgcc cgtgtcgccg cgggcggcgc gcagccgcgc ccgcgcgtgg
     5761 atgacggggt agaaggtgag gtcgtcgccg gcggcgcgct cggccgctcc ggcctcgatg
     5821 agaatccgtt cggcgtcgtc gagctcgcca cgcgcgatca ggacgtcgag cacgtagccg
     5881 agcatcggcg gcgcgagcgc gaaccagccc tgcgcgagcg acagctcggc gcagctgcgc
     5941 gcgtcggcct cggcctcggc cagctctccg aggcgcgcca gcagcatgca gcgccagccg
     6001 gagctgcacg cgaacgcgag ggccgagccc tgccgccggg cgtccgctac cgcttggtcg
     6061 agcgcctcgc gggcggggcc gttggcgtcg gcgagcgcca gcacctcgac cgcggtgaag
     6121 aacggagtgc ccgcgaaccc gcttggcgcg ccggccggct cgcgcgcgcc gagcgcgcgc
     6181 tcggcggcgg ccgccagggt cgcggcgggg gagtcgccgt acatcgcgtc cacgtaggcc
     6241 tgggtcgcga tcagccgcca gccggcgggc gtggccggct ccgcggcggc gtcgtggcgg
     6301 cgcagccgtg cgcgcgccca ctcgaggcgc gcgaggtcga gcgacgcggt cgcgatcagc
     6361 gtcgtctcga gcagcagcgc cagctccggg tccgcgtccg cgacgtcctc gatcgcgcgc
     6421 tcgcgcaccg cgtaggactc ctggtggcgg ccggccgcct ggagcgcggc cgaccactcg
     6481 accgcgcagc tcgcgcgcac ccggggatcg ccggccagcc gcgcgccctc ctcgaagtgc
     6541 tcggccgccg ccacgggctc cccggcgtgc agctccgccg aagcgagctc gagcgtgagc
     6601 gcggcgcggt cgtccgtggc gggcggctcc gccagggcac gccgcaggca tgccgcggcg
     6661 gcgggagcgg ccccgcgggc gagcgcgtcg cgtgcggcct cgcgcagggt cgccaccacc
     6721 cggggctccg ccgccggctc ggtcgcgagg aggtgcaccg cgaccgcgtc ggcgcccgcc
     6781 ccctcggcgg caagcacctc agccgcgtgc cggtgcgcgt cagcccgctc gcccgccgcg
     6841 atgtcggcgt acaccgccga gcgcaggatt ggatgggcga acgccagggg ccgcgccgcg
     6901 gcgaggatgc ccgcgccggc gaggtgctcg gccgcggcag ccgcaagatc ctcgcccagc
     6961 tcggcgagcg cggccgcgcg ccgcagcgga gcaccgtcgc cgagcacggc cacggcgcgg
     7021 gcgagcgcgg ccgcgtcggg gcccagccgc gccacgcgca ggagcaccgc acggcccacc
     7081 gtcggcggcg cgagctggcg cacgagcggc gcggccgccg ctgccggcgc cacgccgtcg
     7141 gcggccagct cgcgcagcag ctcgcggagc aggaacgggt tgccgccggt cgccgcgcgg
     7201 cacgcgtcgc agaacgcgcg atcggggtcc ggcccgagcc gcgcggccac cagcgcgggc
     7261 accgcgtcct cgctcagcgg cggcggccgc aggacgaccg cccgcgcgtc ggtcgcgagc
     7321 gcgtcgatcg agcgccggtc ggagcccgcc tcgtccgggc gggccgccag cgccacgagc
     7381 acgggcagct cgtccagccg cgggagcagg aactggagaa agcgcagcga gctcgcgtcg
     7441 gcccagtgga ggtcgtcgat cacgagcgcg gcgggaccgc gttcggtcag gttcgcggcg
     7501 agccagtaga gcgcgtggaa gtgcggcagc gacgggtcgt ccgcggcggg agcgccgccg
     7561 ggctcgggcg ccgcgaggcc cagcagcgga gcggcggcgg cggcggcgcc gtgcagcagc
     7621 gcgcggcggg tggccgcgtc ggcgcccgcg agcagcggct cgaagagctg gcggaccacc
     7681 ccgagcggga agtcgcgatc gagctcggat gcgcgcgcgg acagcacggt catgccgtgc
     7741 tcgcgcgagc gctcgcgcac cacctcgagc aggcgtgtct tgccgatccc gggctcgccc
     7801 tcgacgagca cgacccgccc gcgaccggcg cacgcctcgc cgatctcgcg ctccaagtcg
     7861 cgcagctcgc tctctcgctc gagcaacatc tcgccgccgc ctcgctccat cgcacgcacc
     7921 cttgcacggc tcgcgggcac ctgtgcgcgg aacccgaaac ctcggggtgc tggttcaggg
     7981 tgctccccgt tgtgagcgct ctggccgggt gggactctcg gtccatgacg aatgcggagc
     8041 agccgaggcg gctcgccctc gaggtgcggt tcgacgccga gccgatcgag ggccgggtgt
     8101 cagagggcga cgggacggcg cggctcgacc gcccgttctc cggctggctc gggctgatgg
     8161 cggcgatcga cgccgcgcgc ggcacggatg cgcgagagca gaggggggag cagcgatgag
     8221 gcgacggaac ctgtggctca cggtggggtt ggcggcggcg gccgtggggc tcgcaccggg
     8281 gagcgccggg gcggcggggt ttgcggagac cgcgggctgc gacgagcagc aggcgttcgt
     8341 ggagggcgac gaggcggcgg tgcgcgcccg gttgcccgag cgctacgcgc ccgtgcgaac
     8401 gccggccggc cggccgctcc tgttcgtgcg cgcgctccgc tgcgacgcgg tcacgctcgg
     8461 cgagcgctca cggcaggctg tcatggccag ctacggcgtg gtcgtggagt cgccggacgg
     8521 agagggctgc gcctcggacg cgccggtggc cggcaccgtg aagggcgacg agccgccggt
     8581 ctgcaactgg tacacgctgg cctggctctc caacgacagg cgggtcgtgg actggctgcg
     8641 cgagggcacc ccgagcttcc cggcgttcca cgtcgagcag ctcgcgttcg acctcaccgc
     8701 gttcggcgcg ggccgcgccg gcgccgcgtt ctccttcagc gccgcgtcgc cctcgccgtc
     8761 gccgttcgcc atggaggcga ccacgcgacc gcggccgggg cccctgtcgg tccgcggcgg
     8821 ctactggttc gacaccccgc aggggaccgt caaggtcgcg ctgtccaccg acgacctcac
     8881 gtcgggcgac gcgacgggcg tggcgcgcgc gggccgcggc tcggagctgg cggcgctgct
     8941 cggcgccgag gagcgcccgt acgtgcccgg ctactcggcg ttcgcggccg agcgctggga
     9001 ccacgcgtcc taccgcaagc agctgtaccg cgctccggct ggaccccgcg cgggggccgg
     9061 cgacggcttc gcgggctcgt gctcggtgca gggaacggtg accttcaagc cgcccgcgag
     9121 ctactaccgg cagccgctcg actacgccta cgagtccgcc ggcacgtgca gcgggacgct
     9181 caacggccgg gagatcgcgg acgtgcccgt caagctgcgc caggccggcc gcagcgaggg
     9241 gagctgcgcg tcggcacgga ccatctcgcc gggcaccggg tcgatcgtgt tcgcgggcgg
     9301 cgagctgatt cgctacacgc tggacttcag ctcgaccggg accgagatcg acttcacgct
     9361 ctatggcgag cgctcgggcc aggcgagcgg gaaggggagc ttcctgacgc agcgaacgcc
     9421 gcccgacgtg acggcgcgct gcgcgagcga gggcgcgtcc gaggtgccga tggacatgac
     9481 gctgaccacg gagagccagc tcgtgagcga gccgccggcg cggacgcacc ccggcacgcc
     9541 ggtgcgcccg cagctgcggc tcgccgtctc gccgcgcgcc gtgcgcgcgg gccgcggcat
     9601 gcgctacgcc ttccgcgtga cggcggccgg gcggccggtc cgcggcgcgc tggtccgctt
     9661 cgcggggcgg gcgatccgct cggacgcggc gggccgggcg gcgctgcggg tgaggctgca
     9721 ccggcccggg ccgcacccgg cgcgcgccgt gaagccgggc tttctgagcg cgcggagcgc
     9781 cgtgacggtg acgcgccgct agcgctcctc cgccgccgcg tagccgggcc agagccgctc
     9841 gagctgcgtg cgcagccagg ccaccgccag gtccttcgcc ttcgcctcga tcatcacgtc
     9901 caccggccgg gccgcggcgt cgagcacctc gcggaggtcg aacggggtca cgaagtcggc
     9961 gtgctggtcg agcagcggag cgcggccgcc cgccctcagc tcggtccgcg ggctcgagag
    10021 gtgcagcttc gggcgcaccc cggcgggcca ggtggcggcc acctcctcga tcgcccgcgc
    10081 ggggtcctcg tagccggggg cgacgttgca gcggtgatgg tggtggtcgt agatgaccgt
    10141 gcagccgagg cggcggtgca gctcgagcac gtccgccacg tcgaagagcc gctcgtcgtg
    10201 ctcgaccgca aggcgcgccc gggcgcgctc ggagagcagc tcgaacgagc gggcgaagcg
    10261 atcgagcgcg gcggtgcggt cgccgccgag ggcgccgacg tggatcacga cgacggcctc
    10321 gggcccggtc ccgagcgcgt ccagcagcgc cgtgtcctgc tcgaggtcga ggcgcgcctt
    10381 cgcggcgacg tcctcgctct gcgagttcag cacgctgtac tgacccgggt gggtggagag
    10441 ccggaggccg tacgcgcgcg ccttcgcgcc gagctccgcg agggcggcgg cacagtcgtc
    10501 gatctggcgg cggtagtcga gctgcgggag gtccgggtgc gtgccgtacg ggaccgtcga
    10561 gctggacagc cggaacacgc tcacgccgtt cgcgtggagg tggtcgaagg cgccatcgag
    10621 cagctcgatc gaccgggaca ggtgcggacc gctctgccag cggcgcgtgt cggaggtgcg
    10681 catgccgccg gcgcccagga tcttcacggc gaagccgagg cggaaggggg gtgcggggcg
    10741 ggcgccaggg gtcgcgcccg ccccgcgtgt ggcggcgatc aggccgctgg gtcctgagcg
    10801 acctcgccgc gccaggcgcc gctctcggcg ccgcgcgact cgatcagctc cttgaagcgc
    10861 tcgaggtcgg ccttgacccg gcgcttcagc aggcccagcg cgtcgcccgc cttctccacc
    10921 acaccctcgg gctcggcgtc gagctggagc atgatccggc tctggccgtc gtcgaggcgg
    10981 tggaaggtca ctacaccggc gtgctgggtg ccgtcgatgc tccgccaggc gatccgctgg
    11041 tcgggggtct gctcggtcac ctcggcgcgc cactcgcggc gctgcccggc gatctcggcg
    11101 gtccagtgga ggtgcgtgtc gtcgagctgc tcgacggcct cgatgccctc catgaactgt
    11161 gggaactcct cgaactgggt ccactggttg tacgcgttgc ggaccgggac ctctaggtcg
    11221 atcgactcct cgatcgtgga catgctggct gcctccgtgg tcgctctgtt ggcgtgcata
    11281 cggccgcgcg cggttggacg gatcacaccg gtagggtccg gcgcatggcg gggcggatcg
    11341 tgctcttcgg ggctacgggc tacacgggtc ggctgacggc cgaggcactc gccgcgcgcg
    11401 gcgcgcggcc gctgctggcc gcgcgcaacg aggagcggct gcgctcgctc gcggacgagc
    11461 tcggcggggg gttcgacgtg gcggtggccg acgtcgcgcg gcctgactcg gtgcgcgacc
    11521 tggtcgagcg cggcgacgtg ctcgtgtcca ccgtcggccc gttcgcccgc tggggcgacc
    11581 cggcggtcga ggccgcgatc gccaccggcg cgcactacat cgactcgaac ggcgagccgg
    11641 cgttcatccg gcgggtgttc gagcgcttcg gccccggcgc ggaggccgcc ggctgcggcc
    11701 tcgtcaccgc gttcggctac gactgggtgc cgggcaacct ggccggtgcg ctggcgctgc
    11761 gcgaggcggg cgacgcggcg gtgcgggtgg cgatcggtta ctacgcgatc ggccgcagcc
    11821 agttcagcgg cggcacccag gcctcggccg cgggggcgct ggccgagccc gcgttcgcgt
    11881 tccgtggggg gatgctccag accgagcgcg gggcgaagcg ggtcgcgaac ttcatggtcg
    11941 agggcgagcg caggccgggg atctcggtgg ggtcgtccga gcacttcggg ctgccgcggg
    12001 tggcgccggg gctgcgcgag gtggacgtgt acctcggctg gttcgggccg atgtcacggc
    12061 cgatgcaggt gggcgcggcc gtcaccgccg ccgccacgcg gataccgggc gtgaagaccg
    12121 gtctgaacgc gctgaccgcg cgggtttcga aggggtcgac cggcgggccg ggggccgagg
    12181 agcgcggtga gagccgctcg tacgtcgtcg cgcaggcgct cgacgcgggc ggcacgtgcc
    12241 tggccgaggt gcgaatcgag ggcgtcaacg gctacgactt caccgcgcgc atgatcgcct
    12301 ggggcgcgga gcgggccgcg gcgtccgggc tcgaggggac cggcgcgctg gggccggtcg
    12361 acggtttcgg gctcgacgag ctcgagcgcg gcgcggccga ggccggcctg gcgcgcgccg
    12421 gctagccggc ctgcggccgg gcgacctcga gcgagccgtc gtcgcggccg agcagcgcct
    12481 cgtcggccag gccgacgaac agaccgtgct cgaccacgcc cgtgacggcc ttgagcgcgc
    12541 ggccgaggtc gccgggatcg gtgcccggtc cgatctcgca gtcgagcagg tggtggccct
    12601 cgtcggtgac caccggcgcg ccggagtcgt cggtgcgcag ctcggcggcg gggaggaggt
    12661 cgagcacgcg ccgccgcgtg tcgggccagc cgaagcgcac gacctcgacc ggcagcctga
    12721 acgactcccc gagcctgctc acgcgcttgc gctcctccgc cacgaccacg aagcgccggg
    12781 cggcctccac gacgatcttc tcgcgcagaa gcgccgcgcc gccgcccttg atcagggcga
    12841 gctgcggatc gatctcgtcg gcgccgtcga tcgcgaggtc gaggccggtc gcgccgtcga
    12901 gctcgacggg ctcgatgccg tgctcggcgg cgagcgcggc ggtgcggtcg gaggcgaagg
    12961 ccgatcgcac cggcgggccg tcgggccagc gctcggcgat cagctcgatc gtccgccaga
    13021 cggcacggcc ggagccgagg ccgagggtca tccccggctc gacccgttcg acggccgcgc
    13081 gcgcggcggc ctcacgtgcg gcgtcggtgc tcacggcgcg caggatgtca ggccgcgcta
    13141 gcggttgatc gcgacgcgac tggtgtcggc cgccggcggt gctcccgcgg tgcgcgcggt
    13201 cacgtcgatc gtcagcggc
//
LOCUS       DASVPL010000288        11086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_34006, whole genome shotgun sequence.
ACCESSION   DASVPL010000288 DASVPL010000000
VERSION     DASVPL010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34006"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..2098
                     /locus_tag="VF517_16075"
     CDS             149..2098
                     /locus_tag="VF517_16075"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX8054506.1"
                     /translation="MTTQILREHLVDGDLKPGSPIGLRIDQTLLQDATGTMAFMQFEQ
                     LGVPRVRVERAVQYVDHNVIQLDFKNPDDHRMLQSMARKFGAEFSRPGNGICHYVHIE
                     RYARPGGILVGADSHTTTSGALGMIAIGAGGLDVAVAMAGYPYEIACPEVVEVRLEGR
                     LDRPWVQGKDIILELLRRRGVSGGKNRIFEFTGPGTGDLTIPERGTIANMIAELGATS
                     AVFPPDDVTREWLRRQEREDDFVDVGPEEGASYDDVEEIDLGALGPLVAKPSSPGNVV
                     PVEEVAGTELGQVCIGSSVNSGYADLALPGAVLADRDGQIVHPGIAATATPGSRQILS
                     AIAESGVYRQLVEGGVRMLEPVCGPCVGMGQAPPSDSNSLRTMNRNFPGRSGTPEDSV
                     YLCSPAVAAVSMVEGKIADPREYGDPPELCPEPELKPYVEDIHIFQPASEEEAERIEI
                     PRGPNIKTPPEHEPLPESIEARIATVQPDDISTGDLAPDGAEVMAYRSNIPAIAEFTF
                     RHRDPEFRKRLAEWGSGFIVGGENYGQGSSREHAALAPLQLGVKAVFAKSFARIHRRN
                     LVAQGILALTFREDSDYDRAEVGDTWSLPRVREELAEGADEITVQIEGGDEFKVTHDL
                     APKELEILVEGGLLHWLQEHGEQAR"
     gene            2308..3009
                     /locus_tag="VF517_16080"
     CDS             2308..3009
                     /locus_tag="VF517_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8054507.1"
                     /translation="MPLRRTEPDQPGHWTEQPPGESPVPRGRGGREHPQRGISRARRG
                     RRGDILDAALTLFNSTGYARTGVQDIAWEAKASVGSIYHHFGGKEEIAAALYVEGLRD
                     YHRGLLRELELRERESAQVAVERLVVNHLRWVERNRELARFLLTSRDPEVVGASDSEL
                     AGMNARIFEAVGSWIERWVAAGAIRPMPLGLFHAVVLGPSQEFARHWVAGRMKVTIDE
                     AETVLAEAAWKAVEA"
     gene            3006..3833
                     /locus_tag="VF517_16085"
     CDS             3006..3833
                     /locus_tag="VF517_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8054508.1"
                     /translation="MTTYSSLVADARATERALVRVERADAHAVVTMDDPGTLNALSAP
                     MTVQLLQALAELVEDDAVRAVVLTGTDPGFSAGGDLRLMRDHAHPMVDDSDGGATETW
                     RWIRRQFGGVSRTIVKADKPFVAALNGAAAGVGLAFALACDVIVASERAHIVPAFGRI
                     GLVPEVGNSWLMTRRLGYQRAFELFAEGRTLTGAEAAELGLVNEVVAHDELMPAALAH
                     VERMAKLPPHVVPMTKSLLRQTADMTWDQAIAMEEFAEPMCFTTTGHRDAVNELLSR"
     gene            3864..4544
                     /locus_tag="VF517_16090"
     CDS             3864..4544
                     /locus_tag="VF517_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054509.1"
                     /translation="MLKRFVLLTLVLALVAPASASAEVTIKVGGSKYREVLYPGSFRI
                     KGKTGTYRGAVKLEVDEFPFDGAYADGGTVNTTDKGEYVFPNVAPTRNARIRARAGSE
                     VSKATTVFVHPGVKRKERLVADFRVKVSFAYLGHPGFAPPADSFFVYMIKNREKRITR
                     LGGARTMAQIGDGRWRFSGIVKLPSGRNYKYGLLYCTRGLSAAGYGRFYRIDRSCGDK
                     VIPFPNGY"
     gene            complement(4519..4935)
                     /locus_tag="VF517_16095"
     CDS             complement(4519..4935)
                     /locus_tag="VF517_16095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEX8054510.1"
                     /translation="MGRRAGGRDARRHLARAPRGALPARAGLELAIATADLTVIALGR
                     PDPSAGEYIAEIQRRLAAQDAVRYRMHAMGTSLEGSTEDILRVVGELHAVPFEMDVPR
                     VYTVLKLDERRDRPDQTLDDKVASIERRLSTRSGTE"
     gene            complement(4839..6341)
                     /locus_tag="VF517_16100"
     CDS             complement(4839..6341)
                     /locus_tag="VF517_16100"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX8054511.1"
                     /translation="MTVATRRIGVVGAGTMGSGIAQLGCTAGIETWLHDPVPEALERG
                     AERLRAGIAKGAEKGRWSLDADQWLRLAPTLEDLSRCDLVIEAAPERPELKRELFEQL
                     SAIAPEAILATNTSSILITSLAGAAARPENVVGMHFFNPPPLMALVEVIPAEQTGDEA
                     MAVAWEVVERMGKTPIRAYDGPGFLVNRCNRPFGAESLRLLQEGVATHEQIDRIVRLG
                     GGFRMGPFELMDLVGIDVGFEVAKSFTEQSFGEPRWKPSPLQARMVAAGRLGRKTGRG
                     WYEYPPGRPDDPPAPEPGGGEGRVVAIEGAGPLARELRERAARAGFDARQLVAVGGLE
                     EPELIVDASVPAPVTDAGDLPQGPPMIAVCADRSLAARGNPHACGFHAIVPLGDLVEL
                     TRLPTTPDPVADAADDFFRALGFHREWVRDAPGLVLGRIVAQLVNEAGFAVAEGVGSI
                     EDVDLGLKLGMNHPRGPSEWDGEQAAVTLDGIWRERREERYRPAPAWSSP"
     gene            6473..7993
                     /locus_tag="VF517_16105"
     CDS             6473..7993
                     /locus_tag="VF517_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054512.1"
                     /translation="MLAVVMLALAATPAGASKTQESILQDDGLLLTDDAGQRDRSLDE
                     LKALGVDTIRVNALWARIAPERDATVKPNFDSSDPGAYPAANWSALDGLVQGAAARGI
                     GILMTATGPAPAWASPCGDRRTCDPNRDEFKAFVTALGRRYSGSYTVGGSAASGGGGG
                     TLPLPKTNTDLDAIVAQANGEQPIQAAQAGTALPRVARWSIWNEPNQGSWLTPQYTGS
                     SKKPVPASARIYRGLLYAAMDALAASGHGSDQVMIGETAPLGRTGGALATRHRSPLAF
                     YRDLLCINSRGSAISTSSLGCKGTFRRVPAAVAHHPYARAAIGSPTSKAGRDDLPLAS
                     IRNLYRVLDQGAKRKRIGKSAPVFLTEYGIQTKPPDRFGVSFGTQARYINQADWMAYR
                     DGRIRSVSQYELVDPADPDTFNTGLRTSSGDAKPALAAYRLPIWVSRGRVWGQVRAAS
                     GGEAEIQWRSSSKKAFKTYRTVKLNAQGYFDQKVSPSRGQWRLVSGGVAGRTASGY"
     gene            complement(7980..9368)
                     /locus_tag="VF517_16110"
     CDS             complement(7980..9368)
                     /locus_tag="VF517_16110"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8054513.1"
                     /translation="MTDADSARLRELGYTQELARRLGGLDNLALSFAGISPVTGLYAA
                     VLIGTIVAGPGWVWVLPVVLAGQCLLLAVYAELAADFPIAGGAYQWSRRLIGGAYGWF
                     AGWMAICAYAVANTTIAYLAAPWALTLLEIDPTPRAIVITGALLVLFCTLSGMLGIRV
                     LTGAVKAGITAEVIATVGLGLALLLVFREQELAIFTDSLAVPGMLAALAVGGWVFIGF
                     DVSVGHAEETQGAARKVPRAIWISLLAVGALVILNAVAAGLAHPDPALIASGEDLDPI
                     TTAVVTSFGSWSAKPFAAVVLVAFVACGMAAQSLTARTMYSIARDGVLPGSRYLRRAD
                     SRSVPVVATAVTGVIAALGLLLGLHSAAVGTLIAFGTAAIYVSFLLTAVAALVARLRG
                     TWGSGRWRLAVNVAAVVWLAFETVNIAWPRESIAPPGAPIYQVWAAPMVLAAITAIGL
                     AYLLLARPLSNR"
     gene            complement(9365..>11086)
                     /locus_tag="VF517_16115"
     CDS             complement(9365..>11086)
                     /locus_tag="VF517_16115"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8054514.1"
                     /translation="PGDEESIEVLLAAGATAASRAPETAARWFSAALRLLPAGDGERQ
                     VEVRVALANALRAIGELERCRTNLLEAIELLPPDATTRRVELTTFCAGVEHWRGLHDD
                     AHRRLLRAWEELPERGTPAAAALQIELGVDGLHALDIDQALTMGEGALETSRALGDTM
                     LTAAAASALGLIKATAGRFAEAREHRDEAVPIVDAASDEELAPRLEALFHLGWTENYS
                     EQYDASIAHAERGMEIARATGQGRLLGSLQLVRGFPFQMQGRLAECLETCEQAVEAAR
                     LSANPNSLYWALYELAWAHYYAGDLEAAMAAADASLDVDNRLLGGTMPSAGGGPGWVR
                     ACCHNELGNPAAALDALNDLNRPDFHVALQRCFDLEVMALARIALGDLAGARADADAA
                     ETDAAQLDMCLARCVAGRAPRRGAAGRVRRAGGGGRRRRRRGRRGADRCAAPGRVRAR
                     APGPWAGGRGRAVRGDRGTARRRAHARRVRLGARARRGAPRAAQARGALGAARPGRGR
                     GRRDRLAVEARARDRRADRRPHDQPADRGPAVPQQEDGRVPHPQPVLQARRIVARGCR
                     ADRRARA"
BASE COUNT         1636 a         4013 c         3989 g         1448 t
ORIGIN      
        1 ggcccgctcg tgaaccgcga cgcgaagctc gtgggcatga actcggccca gcgcaccgac
       61 gtgcagggac agttctacgc ggtcgggatc gaccgcatca acgagatcgt cccgaagctg
      121 atcgagggcg acgacgtctg ctgagagcct gaccacccag atcctccgcg agcatctcgt
      181 ggacggggac ctgaagcccg gctcgccgat cgggctgcga atcgaccaga cgctgctgca
      241 ggacgccacc ggcacgatgg cgttcatgca gttcgagcag ctcggcgtgc cgcgcgtgcg
      301 cgtggagcgc gcggtccagt acgtggacca caacgtcatc cagctcgatt tcaagaaccc
      361 ggacgaccac cgcatgctcc aatcgatggc ccgcaagttc ggcgcggagt tctcgcggcc
      421 gggcaacggg atctgccatt acgtgcacat cgagcgctac gcgcggcctg gcgggatcct
      481 cgtgggggcg gactcgcaca ccaccacgag cggcgcgctc gggatgatcg cgatcggcgc
      541 cggcggactc gacgtggcgg ttgcgatggc cggctacccg tacgagatcg cgtgtccgga
      601 ggtggtcgaa gtgcgcctcg agggccggct cgaccggccc tgggtgcagg gcaaggacat
      661 catcctcgag ctgctgcgcc ggcgcggggt cagcggtggg aagaatcgga tcttcgagtt
      721 caccgggccg gggaccgggg atctgacgat ccccgagcgc gggacgatcg cgaacatgat
      781 cgccgagctg ggcgcgacct cggcggtgtt cccgcccgac gacgtgacgc gcgagtggct
      841 gcgccggcag gagcgcgagg acgacttcgt cgatgtgggg cccgaggagg gcgcgtcgta
      901 cgacgacgtc gaggagatcg acctcggcgc gctcgggccg ctggtggcga agccctcgtc
      961 cccggggaac gtggtgccgg tggaggaggt cgccggtacc gagctggggc aggtgtgcat
     1021 cggctcgtcg gtgaactcgg gctacgcgga tctggcgttg ccgggagctg tgctggccga
     1081 ccgcgacggg cagatcgtcc atccgggcat cgcggcgacc gccacgcccg gctcgcggca
     1141 gatcctgtcc gcgatcgccg agtccggcgt ctaccgccag ctcgtggagg gcggcgtgcg
     1201 gatgctcgag ccggtctgcg gcccgtgcgt cgggatgggc caggcgccgc cgagcgactc
     1261 gaactcgctg cgcacgatga accgcaactt ccccggccgc agcggcacgc cggaggactc
     1321 cgtgtatctc tgctcgccgg cggtggcggc ggtctcgatg gtggagggca agatcgccga
     1381 cccgcgcgag tacggcgacc cgcccgagct gtgccccgag cccgagctca agccgtacgt
     1441 ggaagacatc cacatcttcc agccggcgtc cgaggaggag gccgagcgga tcgagatccc
     1501 gcgcggtccg aacatcaaga cgccgcccga gcacgagccg ctgccggagt cgatcgaggc
     1561 gcgcatagcc accgtccagc ccgacgacat ctcgaccggc gacctggcgc ccgacggcgc
     1621 cgaggtgatg gcctaccgct cgaacatccc ggcgatcgcg gagttcacgt tccgccaccg
     1681 ggacccggag ttccgcaagc ggctggcgga gtgggggagc ggcttcatcg tgggcggcga
     1741 gaactacggg cagggctcgt cacgcgagca cgcggcgctg gcgccgctgc agctcggcgt
     1801 gaaggccgtg ttcgcgaagt cgttcgcgcg gatccaccgg cgcaacctcg tggcgcaggg
     1861 catcctcgcg ctgacgttca gggaggactc cgactacgac cgggccgagg tgggggacac
     1921 gtggtcgctg ccgcgcgtgc gcgaggagct ggccgagggg gccgacgaga tcaccgtgca
     1981 gatcgagggc ggcgacgagt tcaaggtcac ccacgacctc gccccgaagg agctcgagat
     2041 cctcgtcgag ggcgggctgc tgcactggtt gcaggagcac ggcgagcagg ctcgctgacc
     2101 gcggcgcgcg gccgcgcccg aagtcacttc cgtccccaat ggggacgctt ttgaccaccc
     2161 acggcacccg aagtcggttc cgtgaccagg ggatcgggcg ggtcgctgcc ggtgccgggc
     2221 ggcgcaagcg gtgcggtacg cgcactctcc ccttcactag aacgatattc tagaatctga
     2281 ttctagtgaa agtgagtgtg cgacacgatg ccgctgcggc gaaccgagcc tgatcagccg
     2341 ggccactgga ccgagcaacc gcccggtgag agcccggtcc cgcgcgggcg cggcggtcgt
     2401 gagcatccgc agcgcgggat ctcgcgcgcg cggcgcggcc ggcgtggcga catcctcgac
     2461 gccgccctca cgctcttcaa ctcgaccggc tacgcgcgaa ccggcgtgca ggacatcgcc
     2521 tgggaggcga aggcgagcgt cggcagcatc taccaccact tcggcggcaa ggaggagatc
     2581 gccgcggccc tgtacgtcga gggcctgcgc gactatcacc gcggtctgct gcgcgagctc
     2641 gagctgaggg agcgggagag cgctcaggtg gcggtcgagc ggctggtcgt gaaccacctg
     2701 cgctgggtcg agcgcaaccg ggagctcgcg cgcttcctgc tgaccagtcg cgaccccgag
     2761 gtcgtcggcg ccagcgactc ggagctcgcg gggatgaacg cgaggatctt cgaggcggtc
     2821 ggaagctgga tcgagcgctg ggtcgccgcg ggtgcgatcc gcccgatgcc gctcggcctc
     2881 tttcacgcgg tggtcctcgg gccgagtcag gaattcgcgc ggcactgggt cgccggacgt
     2941 atgaaggtga ctatcgacga ggccgagacc gtcctggcgg aggcggcctg gaaggcggtg
     3001 gaggcatgac gacctactca tccctggtgg ccgacgctcg cgcgacggag cgcgcgctcg
     3061 tccgcgtcga acgcgcggac gcgcacgcgg tggtgacgat ggacgaccca gggacgctca
     3121 acgcgctcag cgcgccgatg acggtccagc tcctccaggc gctcgccgag ctggtcgagg
     3181 acgacgccgt ccgcgcggtc gtcctcaccg ggaccgaccc cggcttctcg gccggcggcg
     3241 acctgcgcct gatgcgcgac cacgcgcacc cgatggtcga cgactccgac ggcggcgcca
     3301 ccgagacgtg gcgctggatc cgccgccagt tcggcggcgt gagccggacg atcgtgaagg
     3361 ccgacaagcc gttcgtggcc gcgctgaacg gtgcggcggc cggcgtcggc ctcgcgttcg
     3421 cgctcgcctg cgacgtgatc gtcgcctccg agcgcgcgca catcgtgccg gcgttcgggc
     3481 ggatcggcct cgtgcccgag gtcgggaact cctggctgat gacgcgccgg ctcggctacc
     3541 agcgcgcgtt cgagctgttc gccgagggcc gcacgctcac cggcgccgag gcggccgagc
     3601 tgggcctcgt gaacgaggtg gtcgcgcacg acgagctgat gccggccgcg ctcgctcacg
     3661 tcgagcggat ggcgaagctc ccgccgcacg tcgtgccgat gacgaagtcg ctgcttcgcc
     3721 agaccgcgga catgacctgg gaccaggcga tcgccatgga ggagttcgcc gagccgatgt
     3781 gcttcacgac gaccggtcac cgggatgcgg taaacgagct gctttcgcgt taaccgggcg
     3841 cgctcgcggg gtacgcccgc gccatgctca agcgattcgt actgctcacg ctggtcctcg
     3901 cactcgttgc gcccgcgagc gcgagcgccg aggtcacgat caaggtcggc ggcagcaaat
     3961 accgcgaggt gctctacccc ggcagcttca ggatcaaggg caagaccggc acctaccggg
     4021 gtgccgtaaa gctcgaggtc gacgagttcc cgttcgatgg cgcctacgcc gacggcggca
     4081 ccgtcaacac gaccgacaag ggcgagtacg tgttcccgaa cgtcgcgccc acgcgcaacg
     4141 cgcggatccg ggcccgtgcc ggcagcgagg tgagcaaggc cacgacggtg ttcgtccacc
     4201 cgggcgtgaa gcgtaaggag cgactcgtgg ccgacttccg cgtgaaggtg agcttcgcgt
     4261 acctcggcca cccgggcttc gcgccgcccg ccgacagctt cttcgtctac atgatcaaga
     4321 accgcgagaa gcggatcacg cgtctcggcg gcgcgcgcac gatggcgcag atcggcgacg
     4381 ggcggtggcg cttctcaggg atcgtgaagc tcccgtcggg acgcaactac aagtacgggc
     4441 tcctctactg cacgcgcggg ctgtccgcgg cggggtacgg ccgcttctac cggatcgacc
     4501 gctcctgcgg cgacaaggtc attccgttcc cgaacgggta ctgagccggc gctcgatcga
     4561 ggcgaccttg tcgtcgagcg tctggtccgg ccggtcgcgg cgctcgtcga gcttcagcac
     4621 ggtgtagacc cgcggcacgt ccatctcgaa cggcacggcg tgcagctcgc cgaccacgcg
     4681 aaggatgtcc tcggtcgagc cctcgagcga cgtgcccatc gcgtgcatcc ggtagcgcac
     4741 ggcgtcctgc gccgccaggc ggcgctggat ctcggcgatg tactcgcccg cgctcgggtc
     4801 cgggcggccg agggcgatca cggtgaggtc cgcggtggct atggcgagct ccaggccggc
     4861 gcgggccggt agcgctcctc gcggcgctcg cgccagatgc cgtcgagcgt cacggccgcc
     4921 tgctcgccgt cccactcgga cggtccgcgc gggtggttca tgccgagctt gagcccgagg
     4981 tcgacgtcct cgatcgaccc gacgccctcc gccacggcga accccgcctc gttcacgagc
     5041 tgcgccacga tccggccgag cacgagcccc ggcgcgtcgc gcacccactc gcggtggaag
     5101 ccgagcgcgc ggaagaagtc gtcggccgcg tcggcgaccg ggtccggggt ggtgggcagg
     5161 cgggtgagct caactaggtc gccgagcggc acgatcgcat ggaacccgca cgcgtgcgga
     5221 ttgccgcgcg cggccaggct gcggtccgcg cagacggcga tcatcggcgg cccctggggg
     5281 aggtcgccgg cgtcggtcac gggcgcgggc acgctcgcgt cgacgatcag ctccggctcc
     5341 tcgagtccgc cgaccgcgac gagctgacgc gcgtcgaagc ccgcgcgcgc ggcacgctcg
     5401 cgcagctcgc gcgccagcgg tcccgcgccc tcgatcgcca cgacgcggcc ctcgccgccg
     5461 cccggctcgg gcgcgggcgg gtcgtcgggg cgcccgggcg ggtactcgta ccagccgcgc
     5521 cccgtcttcc gcccgagccg gcccgcggcg accatccgcg cctgaagcgg gctcggcttc
     5581 cagcgcggct cgccgaagct ctgctcggtg aacgacttgg ccacctcgaa gccgacgtcg
     5641 atcccgacca ggtccatcag ctcgaacggg cccatccgga aaccgccgcc gaggcgcacg
     5701 atgcggtcga tctgctcgtg agtggcgacc ccctcctgga gcagtcgcag cgactccgcg
     5761 ccgaacgggc ggttgcagcg gtttacgagg aagccggggc cgtcgtaggc acggatcggc
     5821 gtctttccca tccgctccac gacctcccag gccacggcca tcgcctcgtc gccggtctgc
     5881 tccgcgggaa tcacctcgac gagcgccatc aacggcggcg ggttgaagaa gtgcattccg
     5941 acgacgttct ccggccgcgc agccgcgccg gcgagcgacg tgatgaggat cgaggacgtg
     6001 ttcgtggcga ggatcgcctc gggcgcaatc gcggagagct gctcgaagag ctcccgcttc
     6061 agctccggtc gctccggtgc ggcctcgatg acgaggtcgc accgagagag gtcctcgagg
     6121 gtgggcgcga ggcgcagcca ctggtcggca tccagactcc agcgcccctt ctcggccccc
     6181 ttggcgatcc cggcacgcag acgctcggcc ccgcgctcga gcgcctctgg gaccggatcg
     6241 tgcagccacg tctcgatgcc cgcggtgcag cccagctgcg cgatcccgga gcccatcgtc
     6301 ccggctccga cgaccccgat tcggcgcgtt gctacggtca ttcgctcagc ggatccaagg
     6361 cggccaggat cataagcccc cgattaagcc gctcggttcg ccgcaactcg cgccgccggc
     6421 gcgtgtcctc cgcggacaac cccctcctat gcacagactc ccactcacat acatgctcgc
     6481 ggtcgtaatg ctcgccctcg cggcgacccc ggccggggcc tccaagacgc aggagtcgat
     6541 ccttcaggac gacggtctgc tgctgaccga cgacgccggc cagcgggacc gctcgctcga
     6601 cgagctgaag gcgctcggcg tggacacgat ccgcgtgaac gcgctctggg cgcgcatcgc
     6661 gccggagcgc gacgcgaccg tcaagccgaa cttcgactcg tccgatccgg gtgcctaccc
     6721 ggccgccaac tggtcggccc tagacgggct ggtccagggc gccgccgcgc gcggcatcgg
     6781 gatcctcatg accgccaccg gaccggcgcc ggcgtgggcc tcgccgtgcg gcgaccgccg
     6841 cacctgcgac ccgaaccgcg acgagttcaa ggcgttcgtc accgccctcg ggcgccgcta
     6901 ctcgggcagc tacaccgtcg gcggctcggc ggcgagcggt ggcggcggcg ggacgctccc
     6961 gctgccgaag accaacacgg acctcgacgc gatcgtcgcc caggccaacg gcgagcagcc
     7021 gatccaggcc gcgcaggccg gcacggcgct gccgcgggtg gcgcgctggt cgatctggaa
     7081 cgagccgaac caggggtcgt ggctcacgcc gcagtacacg ggctcgagca agaagccggt
     7141 gccggcgtcc gcgcggatct accgcgggct gctgtacgcc gcgatggacg cgctcgcggc
     7201 gtccggccac ggcagcgatc aggtcatgat cggcgagacc gcgccgctcg gccgcaccgg
     7261 tggagcgctc gcgacccgcc accgctcgcc gctcgccttc taccgcgacc tgctctgcat
     7321 caacagcagg ggcagcgcga tctcgacgtc ctcgctcggc tgcaagggga cgttccgccg
     7381 cgtcccggcc gccgtggcgc accacccgta cgcgcgtgcc gcgatcggga gcccgacctc
     7441 gaaggccggc cgcgacgacc tgccgctcgc ctcgatccgc aacctctacc gcgtgctcga
     7501 ccagggcgcc aagcgcaagc gcatcggcaa gagcgctccg gtcttcctga ccgagtacgg
     7561 gatccagacg aagccgccgg accgcttcgg cgtctccttc ggcacgcagg cgcgctacat
     7621 caaccaggcc gactggatgg cctaccgcga cgggcgcatc cgctcggtct cgcagtacga
     7681 gctggtcgac cccgccgacc cggacacgtt caacaccggc ctgcgcacga gcagcggcga
     7741 cgccaagccg gcgctcgcgg cctaccggct tccgatctgg gtcagccgcg gccgggtctg
     7801 ggggcaggta cgcgccgcca gcggcggcga ggccgagatc cagtggcgca gctcgtccaa
     7861 gaaggcgttc aagacgtaca ggaccgtgaa gctgaacgcg cagggctact tcgaccagaa
     7921 ggtcagcccc agccgcgggc agtggcggct ggtgtccggc ggcgtcgccg ggcgcaccgc
     7981 tagcggttac tgagcgggcg cgccagcagc aggtaggcga gcccgatcgc ggtgatcgcg
     8041 gcgagcacca tcggtgcggc ccacacctgg tagatgggcg cgcccggcgg cgcgatcgac
     8101 tcgcgcggcc aggcgatgtt caccgtctcg aacgcgagcc agacgacggc cgcgacgttg
     8161 accgcgagtc gccaccgccc ggagccccac gtgccgcgca ggcgcgcgac cagcgcggcg
     8221 accgcggtga gcaggaacga gacgtagatc gcggccgtgc cgaacgcgat cagcgtgccg
     8281 accgccgcgg agtgcagccc gagcagcagc ccgagagcgg cgatcacccc ggtgaccgcg
     8341 gtcgcgacca cgggcacgct gcggctgtcg gcccgccgta ggtagcgcga gccagggagc
     8401 acgccgtcgc gcgcgatcga gtacatcgtc cgcgcggtga gcgactgcgc cgccatgccg
     8461 cacgccacga acgccaccag gacgaccgcg gcgaacggct tcgccgacca ggagccgaac
     8521 gacgtgacga ccgccgtggt aatcgggtcg aggtcctcgc cggaagcgat cagggcgggg
     8581 tcaggatggg cgagtccggc ggccacggcg ttgaggatca cgagcgcgcc caccgcgagc
     8641 agggagatcc agatcgcgcg cgggaccttc cgcgccgcgc cctgcgtctc ctcggcgtga
     8701 ccgaccgaca cgtcgaagcc gatgaacacc cagccgccca ccgcgagcgc ggcgagcatc
     8761 cccggcaccg ccagcgagtc ggtgaagatc gcgagctcct gctccctgaa gaccaggagc
     8821 agcgcgagcc cgagccccac ggtcgcgatc acctcggccg tgatgccggc cttgaccgcg
     8881 ccggtcagca cccggatgcc gagcatgccc gacagcgtgc agaacagcac gaggagcgcc
     8941 cccgtgatca cgatcgcgcg cggcgtcggg tcgatctcga gcagcgtgag cgcccagggc
     9001 gcggcgaggt acgcgatcgt ggtgttagcc accgcgtagg cgcagatcgc catccacccg
     9061 gcgaaccagc cgtacgcgcc gccgatcagg cgccggctcc actggtatgc ccctccggcg
     9121 atggggaagt cggcggccag ctccgcatag acggccagca ggaggcactg tccggccagc
     9181 accacgggga gcacccagac ccagcccggg ccggccacga tcgtcccgat gaggaccgcg
     9241 gcgtacagcc cggtcaccgg cgagatgccc gcgaagctga gcgcgaggtt gtcgaggccg
     9301 cccagccttc gcgccagctc ctgcgtgtag ccgagctcgc gcagccgcgc gctgtcggcg
     9361 tcagtcacgc gcgcgctcga cgatccgcgc gacatccacg cgcgacgatg cgccgagctt
     9421 gaagaacagg ttgcggatgt gggactcgac cgtcttcttg ctgaggaaca gccggtccgc
     9481 gatctgctgg ttggtcatgc ggtcggcgat cagctcggcg atctcgagct cgcgcttcga
     9541 cagcgagccg atcccggcgt ccccggccgc gcccgggccg cgcggctccg agcgcgcccc
     9601 gagcttgcgc agctcgcggc gcgcctcgtc gcgcacgcgc accgagccgc actcgtcgag
     9661 cgtgcgctcg gcgtcgcgca gtacctcgat cgcctcggac cgcgcgcccg cggccgccag
     9721 cccacggccc tggagcacgc gcgcgaacgc gacctggagc cgcgcaccga tcagctccgc
     9781 ggcgtccgcg gcggcggcgc cggcggccgc cgccccccgc ccgtcgcact cggccagcag
     9841 caccgcggcg cggggcccgg ccggccacgc agcgcgcgag gcacatgtcg agctgggcgg
     9901 cgtccgtctc ggccgcgtcg gcgtcggcgc gggcacccgc gaggtcgccc agcgcgatcc
     9961 tggccagcgc catcacctcg aggtcgaagc agcgctggag cgcgacgtgg aagtccgggc
    10021 ggttcaggtc gttgagcgcg tcgagcgcgg cggccggatt gcccagctcg ttgtggcagc
    10081 atgcccgcac ccaccccggt ccccctcccg ccgagggcat cgtcccgccg agcaggcgat
    10141 tgtcgacgtc gaggctggca tccgccgcgg ccatggcggc ctcgaggtcg ccggcgtagt
    10201 agtgagccca ggccagctcg tagagcgccc agtagagcga gttggggttg gccgagaggc
    10261 gcgccgcctc caccgcctgc tcgcaggtct cgaggcactc cgccagccgc ccctgcatct
    10321 ggaaggggaa cccacggacg agctggagcg atccgagcag gcggccctga ccggtcgcgc
    10381 gcgcgatctc catgccgcgc tcggcgtggg cgatcgacgc gtcgtactgc tccgagtagt
    10441 tctcggtcca gccgaggtgg aagagcgcct cgagccgcgg cgccagctcc tcgtcgctcg
    10501 ccgcatcgac gatgggcacg gcctcgtcgc ggtgctcgcg cgcctcggcg aagcgtcccg
    10561 ccgtcgcctt gatgagcccg agggcggacg ccgcggccgc ggtcagcatc gtgtcgccga
    10621 gcgcgcgtga ggtctcgagc gcgccctcgc ccatcgtgag cgcctgatcg atgtcgaggg
    10681 cgtggagacc gtccacgccg agctcgatct ggagcgcggc ggcggccggt gtcccgcgct
    10741 cgggcagctc ctcccacgcg cgcagcagcc ggcggtgcgc gtcgtcgtgc aggccgcgcc
    10801 agtgctcgac cccggcgcag aaggtcgtta gctcgactcg ccgggtggtg gcatcgggcg
    10861 gcagcagctc gatcgcctcg agcaggttgg tgcggcagcg ctcgagctcg ccaatcgcgc
    10921 gcagcgcgtt cgcgagcgca acgcgcacct cgacctggcg ctcgccgtcg ccggccggaa
    10981 gcaggcgcag cgcggcgctg aaccagcgcg ccgccgtctc cggcgcgcga gacgccgccg
    11041 tcgctccggc cgcgagcagg acctcgatcg actcctcgtc gcccgg
//
LOCUS       DASVPL010000289         7496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_230486, whole genome shotgun sequence.
ACCESSION   DASVPL010000289 DASVPL010000000
VERSION     DASVPL010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230486"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="trpB"
                     /locus_tag="VF517_16120"
     CDS             <1..1022
                     /gene="trpB"
                     /locus_tag="VF517_16120"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX8054515.1"
                     /translation="RDFVGRPTPLYYAERLSARVGREVWLKREDLTHTGSHKINNALG
                     QSLLARRMGKRRIIAETGAGQHGVATATVCALLDLECVVYMGTEDMRRQRPNVERMRL
                     LGAEVAAVEAGARTLKEAVSAAIRDWVANVGDSHYIIGSAVGPAPYPALVRDLQRVIG
                     DEARAQFLEQAGRLPGRAIACVGGGSNAIGTFVPFVDDPVVLVGVEAAGEGIESGRHG
                     APLATGARGILHGALSAVLQDENGQVLEAHSISAGLDYPGSGPQHAHLRDSGRVEYVA
                     VDDDAAVDAFRWFTEAEGIIPALEPAHAIAWLLAEPPGDGGVDLLTLSGRGDKDLAEV
                     LERSG"
     gene            1019..1798
                     /gene="trpA"
                     /locus_tag="VF517_16125"
     CDS             1019..1798
                     /gene="trpA"
                     /locus_tag="VF517_16125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX8054516.1"
                     /translation="MSAPAARIDAAFAQNGKSAALMPYLMGGFPDVETSLAAGLACAD
                     AGADLIELGIPFSDPLADGPTIHAAGTRALAAGVTPKDVLGVCERIAERIPVVFMVYA
                     NLALQDGFADRALAAGASGLIVPDLPHDEAGQLRAACDERGLALVPLVAPTTTGERMD
                     AIGADARGFVYTVSLTGTTGERDELPPGLAGTVERVRAAASVPVAVGFGISTPAHARA
                     VAELADGVIVGSRVVRAAGEGGAAAVGELVGELAGALTAST"
     gene            complement(1795..2598)
                     /locus_tag="VF517_16130"
     CDS             complement(1795..2598)
                     /locus_tag="VF517_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054517.1"
                     /translation="MRRTLLTLLAAALLTAGCGGGDDGPQSPQERLEAALPDYERALA
                     EQDCRAFARYAHSAVRPPGRGTGDPPDAAECRSLGVGYTRLEGFKAARTKVFGNAALV
                     EGTIDGQLVALVWVVDVDGRWKQVQATPPSISPQITGPPRPQNRFAPNAAAFVAAMRG
                     GDCAKVFRLLSPAAPFLEDENEEEGPFCTRFRRSFRAPQRLAAQLSAAPGAKPIDMGG
                     TADFHFYRLDTGGDRHWTLIMSKLSPATTAGEHLDDSVLDYYPTGLAPR"
     gene            2641..3153
                     /locus_tag="VF517_16135"
     CDS             2641..3153
                     /locus_tag="VF517_16135"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8054518.1"
                     /translation="MTQADTGARAAGEVAQAAFERFIAHDTEGCLAYWHPEGIQDWVA
                     LGVFRGHDEIRALFGEVFAAVPDLEMVVEHVTSDDRRCAIQWRSRGTHDGGPLNGVEP
                     TGRTIEMRGVDVMQVEDGLIVRNTVYYDGAAFARGFGMLPAQDSGAERMMIGAFNGVT
                     RLRRAIAERT"
     gene            3163..3327
                     /locus_tag="VF517_16140"
     CDS             3163..3327
                     /locus_tag="VF517_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054519.1"
                     /translation="MGTVAAFTVGLIVWLVLWANGAKAFDAFLIPVALLLAGATARMV
                     VPYVRERLLP"
     gene            complement(3336..4367)
                     /locus_tag="VF517_16145"
     CDS             complement(3336..4367)
                     /locus_tag="VF517_16145"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX8054520.1"
                     /translation="MRIGVLTGGGDCPGLNAVIRAVVRKGVDRYGHAIVGFRDGWRGV
                     IETSFEELTLESVRGILPRGGTILGSSRTNPFKGDDGATRVRESLAGLNLDGLIAIGG
                     EDTLGAAAKLHAEGVNVVGVPKTIDNDLGGTDRTFGFDTAVQIASDAIDRLHTTAESH
                     HRNLVVEVMGRHAGWIALHCGLAGGADAILIPERPFDIDEVCRLIQRRHSRGRYFAIV
                     VVAEGATPIEGTMATTGAGGTDEFGHVRLGGIGQRLEREIEERTGYETRTTVLGHVQR
                     GGTPTAYDRVLATRFGLAAVDAVSEGRWGTMAALRGEQIEMVSLADAVSQLRTVPAAD
                     YDQAGVFFG"
     gene            4369..5598
                     /locus_tag="VF517_16150"
     CDS             4369..5598
                     /locus_tag="VF517_16150"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8054521.1"
                     /translation="MTATYPARWLASLALLVLAATGAGCGGGDDPSPPRDGALTIYSS
                     LPRQGILARAAGAVAAGQRLALADARGRAGGRAVRLVELDSAGGEDGPWDPDAIEENA
                     RRAGEDQSAIAYLGELDLGGSAVSVPVTNAEGLLQVTPGDGLPSLTRTAPSGGEPPVR
                     YYPEGRRNFIRVVPHAGLQAQALVAWARERGARSIAIVRDESVFGRELAEWLLDLAER
                     ARLPAETERISAEIDDHEAVARDVAERRPGAVLLTALAGRDTDLTVAALRRVLPAAPL
                     LATSAVAPDPPDGVDFLDPRLPAREYGAAAGRILRRLERAAGAPVGPAALYGYEAMRL
                     VLDAVDRAGARPGDRAAVLRAAAGPRPVDDVLGRYFLAPDGDVSTSAFGAYRSERGRV
                     RALGVRSAEAGGTRPPP"
     gene            5595..6515
                     /locus_tag="VF517_16155"
     CDS             5595..6515
                     /locus_tag="VF517_16155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8054522.1"
                     /translation="MRVGYGLAALAATLWAINASMASSLMDRGLGWAPLSQLRVAGAF
                     AILLVGLAIVRRDQLRVERRQVRELAFLGVFALAPVQATYFLAIDRLQIGVALTIQYL
                     APLIVLVWLRLWLGRRIASGLWGAVALSAAGCFLVVRAYDASSLDALGVLVAFASAFT
                     FAGYMVGSERAGRDHSPFTTLVYGFGFASLAWAVAQPWWNFPFDELSAPRDALLALGV
                     VVLGTLFPFLAMVAALRHIPTPRAGIVATLEPVLAALFAWVLVDESLVALQVGGMALV
                     VVAVAWVQGHRPDLEAEAAPGALDQRSKPA"
     gene            6624..>7496
                     /locus_tag="VF517_16160"
     CDS             6624..>7496
                     /locus_tag="VF517_16160"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8054523.1"
                     /translation="MIAALIAAFTMGVAACGGDDKGGGGGKIEGGTLTIYSSLPEQGA
                     SGGQTKAMENGATLAVETKGGKVGKYTVKYKPLDDSLASTGAADEGKARQNARTAVSD
                     KTTAGYIGEYNSGISKVTIPILNRAGIAQISPANTYVGLTTNKPGSEPGEPDKYYPTG
                     KRTYARVVPADTIQGAALAQASQQAGCKSIHIWNSKTTYSTGLARNVTMAAEKIGLEV
                     QGNDGIDPKAPNYRSLAENVKADCFILTGEIESNGVQAVKDVASANPNAKLFAGDGMC
                     LGDTADPKKGLPADI"
BASE COUNT          951 a         2755 c         2763 g         1027 t
ORIGIN      
        1 tgcgcgactt cgtcggccgg ccgacgccgc tctactacgc ggagcgcctg tcggcccgcg
       61 tgggccgcga ggtgtggctc aagcgcgagg acctcaccca cacgggctcg cacaagatca
      121 acaacgcgct cggccagtcc ctgctcgcgc gccgcatggg caagcggcgc atcatcgccg
      181 agaccggcgc cgggcagcac ggggtcgcga ccgcgaccgt gtgcgcgctg ctcgacctcg
      241 aatgcgtggt ctacatgggc accgaggaca tgcggcgcca gaggccgaac gtcgagcgga
      301 tgcgcctgct gggggcggag gtcgccgccg tcgaggccgg ggcgcggacg ctcaaggagg
      361 ccgtgagcgc ggccatccgc gactgggtcg cgaacgtggg cgactcgcac tacatcatcg
      421 gatcggccgt cggtcccgcg ccgtatccgg cactcgtgcg cgacctccag cgcgtgatcg
      481 gcgacgaggc ccgagcgcag ttcctggagc aggcggggcg ccttccgggc cgcgcgatcg
      541 cgtgcgtcgg cgggggctcc aacgcgatcg ggacgttcgt gcccttcgtg gacgacccgg
      601 tcgtgctcgt cggggtcgag gcggcgggtg aggggatcga gtcgggtcgc cacggtgcgc
      661 cgctcgcaac gggcgcgcgc gggatcctgc acggcgcgct gtcggccgtg ctccaggacg
      721 agaacgggca ggtgctcgag gcgcactcga tctccgccgg gctcgactac ccgggctcgg
      781 ggccgcagca cgctcacctg cgcgacagcg gccgggtcga gtacgtcgcc gtggacgacg
      841 acgcggccgt ggatgcgttc cggtggttca ccgaggccga ggggatcatc ccggcgctcg
      901 agccggctca cgcgatcgcg tggctgctcg cggagccccc cggcgacggc ggcgtggacc
      961 tcctcacgct ctcgggccgc ggcgacaagg acctcgccga ggtgctggag cgcagcggtt
     1021 gagcgccccc gcggcgcgca tagacgcggc cttcgcccag aacggcaagt cggcggctct
     1081 gatgccctat ctcatgggcg gctttccgga cgtggagacc tcgctggccg ccgggctcgc
     1141 gtgcgccgac gccggcgccg acctgatcga gctgggcatc ccgttctcgg acccgctcgc
     1201 cgacggcccg acgatccacg ccgccggcac ccgggccctc gccgcgggcg tcacccccaa
     1261 ggacgtgctc ggggtctgcg agcggatcgc cgagcgcatc ccggtcgtgt tcatggtcta
     1321 cgccaacctc gcgctccagg acggcttcgc cgaccgcgcg ctcgccgccg gcgcgagcgg
     1381 cctgatcgtc cccgacctgc cgcacgacga ggccggccag ctgcgcgccg cctgcgacga
     1441 gcgcggcctc gccctggtcc ccctggtggc gccgaccacc accggtgagc ggatggacgc
     1501 gatcggggcg gacgcgcgcg gcttcgtcta cacggtctcg ctcacgggca cgaccggcga
     1561 gcgcgacgag ctgccgcccg gcctggccgg cacggtcgag cgcgtgcgcg cggccgcttc
     1621 ggtgccggtc gcggtcggct tcgggatctc cacccccgcc cacgcgcgcg cggtggccga
     1681 gctggccgac ggagtgatcg tcggcagccg cgtggtgcga gcggcgggcg agggcggcgc
     1741 ggcggcggtg ggggagctcg tgggcgagct ggccggagcg ctcacggcta gcacctagcg
     1801 cggggccagg cccgtcgggt agtagtcgag cacggagtcg tccaggtgct cgcccgcggt
     1861 cgtggccggc gagagcttcg acatgatcag cgtccagtgg cggtcgccgc cggtgtcgag
     1921 gcggtagaag tggaagtcgg ccgtgccgcc catgtcgatc ggcttcgccc ccggcgccgc
     1981 cgagagctgt gccgccaggc gctggggcgc tcggaacgag cgccggaagc gggtgcagaa
     2041 ggggccctcc tcctcgttct cgtcctcgag gaacggcgcc gcggggctca gcagcctgaa
     2101 caccttcgcg cagtcgccgc cgcgcatggc ggcgacgaac gccgccgcgt tcggggcgaa
     2161 ccggttctgc ggccgcggcg gccccgtgat ctgcggggag atcgagggcg gcgtggcctg
     2221 cacctgcttc cagcgtccgt ccacgtccac gacccacacg agcgcgacca gctgcccgtc
     2281 gatcgtcccc tccaccagcg ccgcgttgcc gaacaccttg gtccgcgcgg ctttgaagcc
     2341 ctcgagccgg gtgtatccga cgccgaggct gcggcactcc gcggcgtccg gcgggtcgcc
     2401 ggtcccgcgt cccggcggcc ggaccgcgct gtgcgcgtag cgcgcgaagg cccggcagtc
     2461 ctgctcggcc agcgcgcgct cgtagtcggg cagcgccgcc tcgagccgct cctgcggcga
     2521 ctgcggcccg tcgtcgcccc cgccgcagcc ggccgtcagc agcgccgccg cgaggagcgt
     2581 gaggagggtc cgccgcacag gccgtgaggg tacgcggccg ttgatagggt ccggcccggt
     2641 atgacgcagg cggatacagg ggcgcgcgcg gccggcgagg tcgcccaggc cgcgttcgag
     2701 cgcttcatcg ctcacgacac cgagggctgc ctcgcgtact ggcaccccga gggcatccag
     2761 gactgggtcg cgctcggcgt gttccgcggc cacgacgaga tccgcgcgct gttcggcgag
     2821 gtcttcgcgg ccgtgcccga cctcgagatg gtcgtggagc acgtgaccag cgacgatcgc
     2881 cgctgcgcga tccagtggcg ctcccgcggc actcacgacg gcgggccgct caacggcgta
     2941 gagcccaccg gccgcacgat cgagatgcgc ggggtcgacg tgatgcaggt cgaggacggc
     3001 ctgatcgtgc gcaacaccgt ctactacgac ggagccgcgt tcgcgcgcgg cttcgggatg
     3061 ctgccggccc aggactccgg tgccgagcgg atgatgatcg gcgccttcaa cggcgtcacg
     3121 aggctgcggc gggccatcgc cgagcggacc taggaggctc tagtgggaac cgtcgccgca
     3181 ttcaccgtcg gcctgatcgt ctggctcgtc ctgtgggcca acggggccaa ggcgttcgac
     3241 gccttcctga tccccgtcgc cctgctcctg gcgggtgcca cggcgcggat ggtcgtgccg
     3301 tacgtgcgcg agcgcctgct gccgtaggcg gccggctagc cgaagaagac gccggcctgg
     3361 tcgtaatcgg ccgccggcac ggtgcgcagc tgggagacgg cgtccgcgag gctcaccatc
     3421 tcgatctgct cgccgcgcag cgcggccatc gtgccccagc gtccctccga gacggcgtcc
     3481 acggccgcga gcccgaagcg cgtggccagc acgcggtcgt aggccgtcgg cgtgccaccg
     3541 cgctgcacgt gcccgagcac ggtggtgcgc gtctcgtagc cggtccgctc ctcgatctcg
     3601 cgctcgagcc gctgcccgat cccgcccagc cggacgtgcc cgaactcgtc ggtgccgccg
     3661 gcgccggtgg tggccatcgt gccctcgatc ggcgtcgcgc cctcggccac cacgacgatc
     3721 gcgaagtagc gcccgcgcga gtggcggcgc tggatcagcc ggcagacctc gtcgatgtcg
     3781 aacggccgct ccgggatcag gatcgcgtcg gccccgccgg cgagcccgca gtggagcgcg
     3841 atccagcccg cgtgacggcc catcacctcg accacgaggt tgcggtggtg gctctcggcg
     3901 gtcgtgtgca ggcggtcgat cgcgtcgctc gcgatctgga ccgccgtgtc gaacccgaag
     3961 gtccggtcgg ttccgccgag gtcgttgtcg atcgtcttcg gcacgcccac cacgttcacg
     4021 ccctcggcgt gcagcttggc cgcggcgccc agcgtgtcct cgccgccgat cgcgatcagc
     4081 ccgtcgaggt tcagccccgc gagcgactcg cgcacgcgcg tggcgccgtc gtcgcccttg
     4141 aacgggttcg tccgcgacga gcccaggatc gtccccccgc gcggcaggat gccgcggacc
     4201 gactcgagcg tgagctcctc gaacgaggtc tcgatcacac cgcgccagcc gtcgcggaac
     4261 cctacgatcg catggccgta gcggtcgacc cccttgcgga ccacggcgcg aatcaccgcg
     4321 ttgagcccgg ggcagtcgcc gccccctgtg agcactccga tgcgcaagat gaccgcaaca
     4381 taccccgcgc ggtggctggc ttcgctggcg ctgctggtgc tcgccgcgac gggcgccggc
     4441 tgcggcggag gggacgaccc gagcccgccc cgcgacggcg cgctcacgat ctactcgagc
     4501 ctgccgcgcc agggcatcct cgcgcgcgcg gccggcgccg tggctgccgg gcagcggctg
     4561 gcgctggctg acgcgcgcgg tcgcgcgggc gggcgcgcgg tgcgcctcgt cgagctcgac
     4621 agcgcgggcg gcgaggacgg gccgtgggat cccgacgcga tcgaggagaa cgcccgccgc
     4681 gccggcgagg accagagcgc gatcgcctac ctcggcgagc tcgacctcgg ggggtcggcc
     4741 gtgtccgtgc ccgtcacgaa cgcggagggg ctgctccagg tgaccccggg cgacggtttg
     4801 ccgagcctca cccgcaccgc cccgagcggc ggcgagccgc ccgtccgcta ctaccccgag
     4861 ggcaggcgca acttcatccg cgtggtcccg cacgcggggc tccaggcgca ggcactggtc
     4921 gcgtgggcgc gcgagcgcgg tgcccgctcg atcgcgatcg tgcgcgacga gagcgtgttc
     4981 gggcgcgagc tggccgagtg gctgctcgac ctcgccgagc gcgcgcgcct gcccgcggag
     5041 accgagcgca tctccgccga gatcgacgac cacgaggccg tggcgcgcga cgtggccgag
     5101 cgccgccccg gcgcggtgct gctcaccgcg ctcgccggcc gcgacaccga cctgacggtc
     5161 gcggctctgc gccgtgtgct gccagccgcg ccgctgctgg ccaccagcgc ggtcgccccg
     5221 gacccgcccg acggcgtcga cttcctcgac ccgcggcttc ccgcgcgcga gtacggcgcc
     5281 gccgccggcc ggatcctgcg ccggctcgag cgggccgcgg gcgcgccggt gggcccggcg
     5341 gcgctgtacg gctacgaggc gatgcggctc gtgctcgacg cggtcgaccg ggccggtgcg
     5401 cggccgggcg accgcgccgc ggtgctgcgc gcggcggccg gcccgcggcc ggtcgacgac
     5461 gtgctcgggc gctacttcct cgcgcccgac ggcgacgtgt ccacctcggc cttcggcgcc
     5521 taccgatccg agcgcggccg cgtgcgcgcg ctaggcgtgc gcagcgccga ggccggcggg
     5581 acccggcccc cgccgtgagg gtcggctatg ggctcgccgc gctcgcggcg acgctctggg
     5641 cgatcaacgc gagcatggcc tcctcgctga tggaccgcgg tctcggctgg gcgccgctct
     5701 cgcagctccg cgtggccggg gcgttcgcga tcctcctggt ggggctggcg atcgtccggc
     5761 gcgaccagct cagggtcgag cgccgccagg tccgcgagct ggccttcctc ggcgtcttcg
     5821 cgctcgcgcc ggtgcaggcc acctacttcc tcgccatcga ccgcctccag atcggcgtgg
     5881 cgctcacgat ccagtacctc gcgcccctga tcgttctcgt ctggctgcgg ctctggctcg
     5941 ggcgccggat cgcctcggga ctctggggcg cggtcgccct gtcggcggcg ggctgcttcc
     6001 tcgtcgtccg cgcctacgac gccagctcgc tcgacgcgct cggggtcctg gtcgcgttcg
     6061 ccagcgcgtt caccttcgcg ggctacatgg tcggctccga gcgcgccggg cgcgaccaca
     6121 gcccgttcac cacgctggtc tacggcttcg gcttcgcctc gctcgcgtgg gcggtcgcgc
     6181 agccgtggtg gaacttcccg ttcgacgagc tgtccgcgcc ccgcgacgcg ctgctggcgc
     6241 tcggcgtcgt cgtgctcggc acgctgttcc cgttcctcgc gatggtggcc gctctgcgtc
     6301 acatccccac accccgcgcg ggcatcgtcg cgacgctcga gccggtgctc gccgcgctct
     6361 tcgcgtgggt cctggtggac gagtcgctcg tcgcgctcca ggttggcggg atggcgctgg
     6421 tggtggtcgc ggtcgcctgg gttcagggcc atcggccgga cctggaggcc gaagccgcgc
     6481 ccggcgcatt ggaccagcgg tccaaacccg catgagagag gggttgcgtc tatgtcatgt
     6541 ctatgacgcc ggaggtgtac gatcccgccg tctccgagct acggaaatca ggaggggagc
     6601 acaacttgcg agcacgatgg tttttgatcg cggcactaat cgccgcgttc accatgggcg
     6661 ttgccgcctg cggtggcgac gacaagggcg gcggtggtgg aaagatcgag ggcggcacgc
     6721 tcacgatcta ctcgagcctc ccagagcagg gcgcgtccgg tggccagacg aaggccatgg
     6781 agaacggcgc cacgctcgcc gtagagacga agggcggcaa ggtcgggaag tacacggtca
     6841 agtacaagcc gctcgacgac tcgctggcct cgaccggcgc cgccgacgag ggtaaggcgc
     6901 gtcagaacgc gcgcacggcg gtgtccgaca agacgaccgc cggttacatc ggcgagtaca
     6961 actcgggcat ctcgaaggtc acgattccga tcctcaaccg ggccgggatc gcccagatca
     7021 gccccgcgaa cacgtacgtg ggcctgacga ccaacaagcc gggctcggag ccgggcgagc
     7081 cggacaagta ctacccgacg ggcaagcgca cgtacgcgcg cgtcgtcccg gcggacacga
     7141 tccagggtgc tgcccttgct caggcatctc agcaggctgg ctgcaagagc atccacatct
     7201 ggaactccaa gacgacctac agcacgggtc tcgcccgtaa cgtgaccatg gcggccgaga
     7261 agatcggtct cgaggtccag ggcaacgacg gaatcgaccc gaaggcgccg aactaccgct
     7321 cgctcgccga gaacgtgaag gctgattgct tcatcctcac gggcgagatc gagtcgaacg
     7381 gcgtacaggc cgtcaaggac gtcgcgtccg ccaacccgaa cgcgaagctg ttcgcgggcg
     7441 acggcatgtg cctgggcgat acggcggacc cgaagaaggg cctgccggcg gacatc
//
LOCUS       DASVPL010000290        21258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_270677, whole genome shotgun sequence.
ACCESSION   DASVPL010000290 DASVPL010000000
VERSION     DASVPL010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 21258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21258
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270677"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VF517_16165"
     CDS             <1..346
                     /locus_tag="VF517_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054524.1"
                     /translation="PAWPARLGRFPIAVDASGDHGGLHTAIRSTAANGTCTSVAVYFE
                     PATPVPLLEMYTRGCTFHTSRVHARALIPEVLALIVAGRLDPQPVTSAVVGFDDAPEA
                     LADPPTKLILTP"
     gene            458..1384
                     /locus_tag="VF517_16170"
     CDS             458..1384
                     /locus_tag="VF517_16170"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX8054525.1"
                     /translation="MTTAQQSTPPPPEPQPRRLVRSRKRMIAGVGGGLGRYFGIDPVI
                     FRIALVALLFFGGSGIFVYAAMWLFVPAEDATRPPLGVRFFAGDRAVWKRVGLILAVV
                     VGSALAAAGSFWATGTGSGAIVAGVVIVLGLALVAAALRGGARWLILPALAVALPAGV
                     VSAADVDLHGGTGERTHRPHSIQDMREGYRLGMGDMTVDLRDVKFPPGDRKLDLEIGT
                     GHIELIVPADVCVVTKARVGAGYVGALDRDSGGLDVEWDDAPVPPAGVPRLVVDADVG
                     IGAIMIGNRPMGDDFQPGRFGSNEACSRQPAR"
     gene            1381..1569
                     /locus_tag="VF517_16175"
     CDS             1381..1569
                     /locus_tag="VF517_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054526.1"
                     /translation="MSRRALDLTSLVAGVAIAVLGALLLLDQEDAIDLGFAYLAPAVT
                     ATVGAILLASGLSRSRRG"
     gene            1599..2846
                     /locus_tag="VF517_16180"
     CDS             1599..2846
                     /locus_tag="VF517_16180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX8054527.1"
                     /translation="MGAPASPAAPARRAEVRRDSQHGIVAGVGAGIARALGVDPVIVR
                     VVFGVAAVVGGIGLIAYALGWLLIPASDGGTLAGRVLSRRGSVEVATGAGLLGLSAML
                     LLRALGWWFDDAVAWPVVAAAVGVALLWQQSAGGSAEAATADEPVRGEIRREPLPFLG
                     GRARSTVALGAALVLGAALVFLWANGAFDAAGDVVLAAVVVVVALGLILAPLWWRLGR
                     SLATERAERIRSEERAELAAHLHDSVLQTLALVQRRADQPREVAALARRQERELRAWL
                     LRRPPAGEGTSLSAALEEAAAEVESAHGAAIEVVTVGDCPLDARIEPVVAAAREAMLN
                     AAKFAGASGPIDAFAEVSADRVVVFVRDRGDGFDPAAVPEERRGVRESIVGRMERNGG
                     SAVVRSSPGAGTEVELSVARGAP"
     gene            2843..3508
                     /locus_tag="VF517_16185"
     CDS             2843..3508
                     /locus_tag="VF517_16185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8054528.1"
                     /translation="MSAIRVVIVDDHAIFRAGVRSELGERVKVVGEAGTVADAVGCIG
                     DLEPDVVLLDVHMPDGGGVEVIRQVTAADEPAATRFLALSVSDAAEDVIAVIRAGARG
                     YVTKTIAGSELAEAIERVHGGDAVFSPRLAGFVLDAFAGEATPTEADPELDQLTARER
                     EVLQHIARGYMYKEIAHRLGISAKTVEAHVSAVLRKLQLSSRHELSRWAMDRRLVDPG
                     GEP"
     gene            complement(3565..4191)
                     /locus_tag="VF517_16190"
     CDS             complement(3565..4191)
                     /locus_tag="VF517_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054529.1"
                     /translation="MLQRLRGKLTYANVMSSIAVFMAMSGVAWAATLPKNSVGTAQLK
                     SNSVTSAKIKNGQVASSDVKDKALVGADLRDDSVTGDDVNEGTLGTVPKASAADNATN
                     ATNATTADSAQPMAFATVDGATGNISDAKGIIQANIVRVNNGIYCVNNLAFAVKGASV
                     ITQFTGTAGSTAQFSKGPTGNCPSGAQILTYNSSGAAADQTFYVMLWG"
     gene            complement(4333..4426)
                     /locus_tag="VF517_16195"
     tRNA            complement(4333..4426)
                     /locus_tag="VF517_16195"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4391..4393),aa:Sec,seq:tca)
     gene            complement(4643..6238)
                     /locus_tag="VF517_16200"
     CDS             complement(4643..6238)
                     /locus_tag="VF517_16200"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX8054530.1"
                     /translation="MGVRLGDEGNGASAGGRSGISPAPGGRESPLRARRDGMLWRTVS
                     WRTSLLVTLGAALMITVSLGPMAEDLGRFSAVVWLVIALVGALQCLLLAELAARMPGR
                     AGGTATYVHAAVGERAPILGAASSWGYWFAWTPGIAVNLILAATYLRDTVWSGISTLG
                     FALVIGAVLYALNALGLRASMRAAALAALLAVGPLIALVIGVIVQPSLIDLDRLTPLS
                     VPDHSWTAGATWLLIAKWMFVAAWAAYGAEMASTVVAEMREPAEHAPRAMKIAASMGV
                     VVFGALPFFVLALVGVGGLTEDPMAIFLPVAESVFGDAGRTIIGLMLTAALVLGAQAF
                     MVGSSRTIYQMTRDGYLPRFFARLNSRGVPIGSIGCDAVVILLVLLVFGTDVVDVVAS
                     ANVGYLIVFVLLPIAYIVLRRGEAVGGGDAVGDGDGPAGAVSGASGDIGAGGGYRLGR
                     GAVALAAALSAFNAVLLVVGGSQWGAKVVLTGAIVMAAIVPVMMLRRWEDHRGGRTEL
                     LRFPAGAPHDPAEDTSLQPVAGA"
     gene            6345..7760
                     /locus_tag="VF517_16205"
     CDS             6345..7760
                     /locus_tag="VF517_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HEX8054531.1"
                     /translation="MGRGDASHAHERGHITDSRFHGHMYATEASRAIFCDTCRLQRWL
                     DIEVALSASQAELGMIPASAAERIAAAARIELIDLDALQADIRRTHHSLVPLIRALQA
                     GCEEGAGEFVHYGATTQDIQDTGQALEMRDALDELTRVGQSIARTVVALAREHGDAVI
                     IGRTHAQPALPTTFGLKLAGWADELLRDLERIEAMRPRVLTIQLFGGVGTMSAWGEGG
                     SALVERVAERLGLSVPAIAWHASRDRVAEYASTVAMAAATFARIADEIRTLSRPEFGE
                     LELGWHEGQVGSSTMPHKRNPESVQQVVALARLAAGQVPGTLHAMIVEHERDSRTLRM
                     EWPLVADVSHYALAAGAILDTVLGELVVHPEAMLRNAERTAALLATESLMLALAGRMG
                     KQSAYKLVYGLSQAATSAGRHVRDELAADEQVREHLTEQELDEVFDPARHVGDAHAMV
                     ERALAHAEERLGVADEVAVGS"
     gene            7757..8347
                     /locus_tag="VF517_16210"
     CDS             7757..8347
                     /locus_tag="VF517_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054532.1"
                     /translation="MSSSPAAAGALDLARLRELRLEREHYDTIVAHGRRKLEGPYLDE
                     ETHERQAFGLLAGHIEGDAARTIQVFPLLRNMRHDPRFRPAMDETVNELGVPSTTPLP
                     RRGWIADPRELLHAHARCEEDGSLIFANYHMHKVGWEHDPLRDTPTALDAALAEGQGV
                     WTLILSLVDPERPLLRAFYEGMKDREARILVAGAPL"
     gene            complement(8358..8675)
                     /locus_tag="VF517_16215"
     CDS             complement(8358..8675)
                     /locus_tag="VF517_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054533.1"
                     /translation="MHEIVLIEDGCCHAGEQDDFVAYLEKEFEGRATVVKYGVGGALG
                     FSAVPAELSNRLLAQGAKAVPVLAVDGNLLFNGEIPERERSVELIEAQMAEPAEREVA
                     AQS"
     gene            complement(8690..9745)
                     /locus_tag="VF517_16220"
     CDS             complement(8690..9745)
                     /locus_tag="VF517_16220"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054534.1"
                     /translation="MFLGNQVPWFAFVVHARDLRDLLTFPGATLIREHSSSDEEFVEK
                     LSTNPPVVLDDVRFGTAAIRGEIIGVPLLPEAIVTPDGHRAVIDAVKLAVQRGSHFVG
                     LGALTSPATAGGRAVVRHVPPTVTVTNGNGLTAAIAHQNVHEARGERAPERTRVAVVG
                     ATGSVGVPLSELLADDGFDLVLIGSSPARAAKVLGGLAERAELAGDLGPVGEADVVVT
                     LTNDPAAKLRPEHLRPGAVVIDVAQPPNVEDSEREELARHGVSMARGGAVRIPGYSCE
                     QDFRLPDRGQTFACLAETYALAKESVRETSVGRPSADYARRMAELAGRQGIATCKLDV
                     EVGTDPDLKTGAMSLST"
     gene            complement(9770..9940)
                     /locus_tag="VF517_16225"
     CDS             complement(9770..9940)
                     /locus_tag="VF517_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054535.1"
                     /translation="MSSSLEDSLEVLREGRSVNSIYNTTFLLKWPDHLGGEATHVTFE
                     GSLVIIPTLGMA"
     gene            complement(9950..10393)
                     /locus_tag="VF517_16230"
     CDS             complement(9950..10393)
                     /locus_tag="VF517_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054536.1"
                     /translation="MATEQISPTIEEIQARRELPEPTQKVAFQIGGLYGISREGLRLP
                     LSKTESIDSGQVAMTLDPQAPSASNFGMVDFENRKLRVQYHVQCLFPGLHDLVTEGKY
                     DRNLLHPIRATATDDCDVYEDYSGWHARGRMDFLPGSMWSGAGGG"
     gene            complement(10420..11349)
                     /locus_tag="VF517_16235"
     CDS             complement(10420..11349)
                     /locus_tag="VF517_16235"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8054537.1"
                     /translation="MSNQAYRRYMLATVLVPPLALLVAVVAFWPRVMTLEDAIVFATM
                     FTISGFGVTIGFHRLLTHRAFKTHKPVRLALATFGTFAAEGPAIVWVAHHRKHHALSD
                     EPGDPHSPHLSGSGNGAALRGLWHAHVGWLLGDRLTSEPLRYAPDLSREREMRWISMH
                     FIHIVIAGVLLAGLLDLALTGTFEGFVRGVLWGGVVRIAAVNHITYSVNSICHCFGSR
                     RFGTADRSRNVPWLALLTFGESWHNNHHAFPTSARHGLRWYELDPSAMVIRAMEMLGL
                     AWDVVKIPKHRVATKESAPRPPGEAAPSGGMVR"
     gene            complement(11349..11609)
                     /locus_tag="VF517_16240"
     CDS             complement(11349..11609)
                     /locus_tag="VF517_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX8054538.1"
                     /translation="MPRTVTIDEATAIIRSKLGSRGARTEIRGSSRLDDLGLSSLEVA
                     DTFFELEELVGHELDATVVADVQTLDEMLAAVNGQADRLQAG"
     gene            11719..12054
                     /locus_tag="VF517_16245"
     CDS             11719..12054
                     /locus_tag="VF517_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054539.1"
                     /translation="MSTGPTAQNDAPAACSLDAAGAARQLDTFAALCERALVDFERGD
                     TSVAFRFRDEEDVRRSIDEYVGVERGCCGFLDFTVEDREGLPVLRIVAGEGTPAAVLH
                     SMYGPPGRA"
     gene            12061..13347
                     /locus_tag="VF517_16250"
     CDS             12061..13347
                     /locus_tag="VF517_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX8054540.1"
                     /translation="MTAAPGDGSTVVHTYSDPEEGFEGYLAYSGEGRPLAAGGFRVQR
                     GLSARTIRELAEAMKLKEGVLQLNVDGAKCGIDYDPGSSGKHAAMRRFLRFLAPHLAG
                     RLSLGPDMGTTFPEIEALAREEGIASVKGAIARAQGLPEEQVLRRLATLDLDSGPLTV
                     GSRRAGHALAHATFAAMRSVGLSSPPATCALEGFGTLGRGAALTLHEAGMVVTAVADE
                     HGCISRAGGLDLDEMLRSPHATPVPELAPPGAAVEDSAAVLALPADVLVLAAREDALS
                     EEDVERVQARVVVVGANNGLRPSVEEFLFERGVLVVPDFVGGSGGSASMDALFGPPLC
                     PDPVTVLTRVAMTVRALVEDIAATSERMQLSPRTAALALAADHPLSPDAKPYGMRLLR
                     DIERVDALSASDVRDRASRVAAGCAWQAQLEHAGLE"
     gene            complement(13351..13599)
                     /locus_tag="VF517_16255"
     CDS             complement(13351..13599)
                     /locus_tag="VF517_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8054541.1"
                     /translation="MTDSSDAAILARIRDHLAVIDPDAAIEAVTLHATIADLDVESMT
                     VMNVVAEIEDDLDVRVPEDELGMVETVRDLVRLFQRQL"
     gene            complement(13596..15254)
                     /locus_tag="VF517_16260"
     CDS             complement(13596..15254)
                     /locus_tag="VF517_16260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX8054542.1"
                     /translation="MAYRRKRVEREGARAEVDGESVATVADVVEALGHEDRGFTFLAG
                     RTERPIAFAELRERARAMARRLRGAGLAPGDRAVLILPSEEEFVQVFLGAVLAGVVPV
                     PLYPPFAGQRMEAYADRIRAVMRVSEAAAVITLPAVAARLAPNAVPSFSFEDLEAMRP
                     AADLPAEPRGEIAFLQFTSGSTADPKGVMITHDAVVANARAISAHLEIHGDRDRVVSW
                     LPMFHDMGLVGMVITPLLEETPTWFMSPLAFARRPPDWCALMSRVGGTVTYAPNFGYD
                     IVAERVRDEDLAGWDLSSWRVAGCGAEPIRADSLRRFAKRLEPAGFSPDALLPSYGLA
                     ESTMVVTATPPGRGMRTLDAGAEGVEPVSCGPALAGHEVTVVGDDGAPLPEGREGSIV
                     VRGPSAAKGYYRDPERSAETFRDGSVVTGDLGFLDGGELFVTGRLKDLLVLRGRNHHP
                     QDLEWCAAELDDVRVAGVVAFSCPASDGAEGAVIVAETDRFRGHGELADAIRKRVLDQ
                     LGVRAADVLVVERGTLPKTTSGKLRRGELRSRYLAGALEPAAAR"
     gene            15317..16309
                     /locus_tag="VF517_16265"
     CDS             15317..16309
                     /locus_tag="VF517_16265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8054543.1"
                     /translation="MSVIETRDLSRRFAGGVAAVDGLTMRVEAGEIFAFLGPNGAGKT
                     TTIRILVTLLKPTGGEATVAGHDVVREAGRVREKIGIALQEAALDPLMTGVECLDLQA
                     SLYGVPDATARQRRDELLESVELVDAASRRVGTYSGGMRRRLDLAMALIHRPEVLFLD
                     EPTTGLDPGSRQTIWERVRELAAAGTTIFLTTQYLEEADRLAGRVMIIDSGNAIAEGT
                     PAQLKAEFGAPRIEITIEERVAEAVLPRLARFGTARVERPGVISLATEGELPSVGAIA
                     GAFEGESACVRSIEVVEPTLDDVFLARTGHQLKESAAEGAAPPDERREAQLPGG"
     gene            16275..17054
                     /locus_tag="VF517_16270"
     CDS             16275..17054
                     /locus_tag="VF517_16270"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8054544.1"
                     /translation="MSGAKRNFRAGSRLARRATAQTLRRPPLVGPILILPTVVLIAFS
                     GGGASAVDLPGFPEVPSFFDFELPGIVTMSAALAGVSGALALAGDLEMTFMDRVLAAP
                     LWRGVPVVGRLAGSAALAVVSCVWFFTLGLIFGDWPSEGVLGLLVIAGLAALTAAAVA
                     SVIATIAIRTRQSSVIQGFFPLMLFFLLLSSAYFPRHLLHEPARTIAGFNPLTAVADA
                     MRDAWIGELSVGATVGAAGAVAALALVGAVGWGLALVGTRR"
     gene            17051..17818
                     /locus_tag="VF517_16275"
     CDS             17051..17818
                     /locus_tag="VF517_16275"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8054545.1"
                     /translation="MRPVGVKRLVRLLRRSLNEVVRVPGVTVQNVLAPTLIMLGTTAV
                     FGTLVEGGGFGTDDYMTFLLPWGFLQGAAFTGAATGVNFARDIERGFVDRLLVAPIAR
                     GLLIVVNALSAAVRTVLPLALLVPVALAFGAELSGPGGVAVAALVAAGFAMLASAWGT
                     FVALVTRTQEAGPMMQAPVLLTIMLTTSFAPEERLAGWMRDIADVNPVTPILELARQA
                     ILSDVLWSDVRPALIAFAGIGVVLYALTWNQMRRALV"
     gene            17828..18583
                     /locus_tag="VF517_16280"
     CDS             17828..18583
                     /locus_tag="VF517_16280"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX8054546.1"
                     /translation="MKPILYVIPGTHPCMTARLMLEHKGIDYRRIDLLPPMHRPFLRA
                     LRFPDETVPALHIGGAKITNTRRISRALEERVPNPPLFPSDPERRAAVEEAERWGDQE
                     FWEGPNRIAWWAVAHDQPSIPSLMEEARVPIRLAPVLAPPIARMGVKRNSADDEHTRP
                     DIEALPAQLDKIEAWIDEGVLGGDELNAADYQIAPSLRIMMCLDDLRPLFDARPVGPY
                     ARRAAPYFPGQIKPVVPEEWLAPLREAAPAAAG"
     gene            complement(18771..19526)
                     /locus_tag="VF517_16285"
     CDS             complement(18771..19526)
                     /locus_tag="VF517_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054547.1"
                     /translation="MKPVWILTCATALALGVTSVATGAVGDPVLIGKRNAALGQTAMV
                     GTSTTYATRQSNNQEGDGGAASYGCRAPLAAEPCLFVLNHRGGEAFYFRSRGGESAGF
                     FEVIPPAGKTADDVRPFTTNATGVATGLNADEVDGKSAADLVSDAVAASQPKLAMVNA
                     AGTEATGRGLAQAAAVQKTGAGTYTVTFASDVSKCALSATEATTTDAGAAAAAPVSGS
                     PNAVTVVTRAGGGADGTEPTARADRPFHLVVNC"
     gene            19668..20303
                     /locus_tag="VF517_16290"
     CDS             19668..20303
                     /locus_tag="VF517_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8054548.1"
                     /translation="MSTQPNQRSVKIADDERGGWSSDRPRKGPPREADMSVRCRVLKP
                     EPQMRYSPGSLLMVVSGSPEERDRFTARVLEGPGLLLSLDKVRGLLTGRGAAEDVAAE
                     RAPELLDTAVMKRLEANETVVVAADGLEAGERERFVRMAAQHRRPRHLILIEAARDSV
                     SDEARPALNDLRRRLDAGELGAEGFQTALRLGGQSVGELKRIVFRPEPRDD"
     gene            20547..21038
                     /locus_tag="VF517_16295"
     CDS             20547..21038
                     /locus_tag="VF517_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054549.1"
                     /translation="MPGIRTTIATAGTLAALGALTAFASSSGQKPESQPAKSSAVPTE
                     VRTKVITRTIRRRERASGGGGSAAPRSGGGGGGGGGARSAGPAPPAPISRAAVSAPPV
                     TAPSGGPSSSGAGQSGGGDDHGRGRGRGRGGDEDRSGHGGGGGGDDHSGHGGGGHGGS
                     GDD"
     gene            21031..>21258
                     /locus_tag="VF517_16300"
     CDS             21031..>21258
                     /locus_tag="VF517_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054550.1"
                     /translation="MTEPTLTDAPAPAPETRVAEHAPTVALPPAAEPAVPAAAQAAEP
                     VEPAAAAAAEAPPAAAAPAAEPVEPAAAAPAA"
BASE COUNT         2791 a         7844 c         7696 g         2927 t
ORIGIN      
        1 gcccgcctgg ccggcgcgcc tcggccgctt tccgatcgcg gtcgacgcca gcggcgacca
       61 cggcgggctg cacaccgcga tccgctccac cgccgcgaac ggaacgtgca cgagcgtggc
      121 ggtctacttc gagccggcca cgccggtgcc gctgctcgag atgtacacgc gcgggtgcac
      181 gttccacacg agccgcgtgc acgcccgcgc gctgatcccg gaggtgctcg cgctgatcgt
      241 cgcggggcgc ctcgacccgc agccggtcac gagcgcggtg gtcggcttcg acgacgcgcc
      301 ggaggcgctc gccgacccgc cgacgaagct gatcctcacg ccgtagcggg cgtcccgccg
      361 gtgggcgggc ggcggatccc gtaggggaca ttccggattg tcggcggacc tcgtcgcacg
      421 gagactcgtc cgtgtcaacg agactgagga gacagacatg acgaccgcac agcaatccac
      481 cccaccgccc cccgagccgc agccgcgccg cctggtgcgt tcgcgcaagc gcatgatcgc
      541 cggcgtcgga ggcggcctcg gtcgctactt cggcatcgac ccggtcatct tccggatcgc
      601 cctcgtggcg ctgctcttct tcggcggctc gggcatcttc gtgtacgccg cgatgtggct
      661 cttcgtcccc gcggaggacg cgaccaggcc gccgctcggc gtgcgcttct tcgccggcga
      721 ccgggcggtg tggaagcgcg tcgggctgat cctggccgtc gtggtcggga gcgccctcgc
      781 ggcggccggc agcttctggg cgaccggcac cgggtccggg gcgatcgtcg cgggcgtggt
      841 gatcgtgctc ggactcgcac tcgtcgcggc ggcccttcgc ggcggcgcgc gctggctgat
      901 cctccccgcg ctggccgtcg cactgcccgc cggagtcgtc tcggcggccg acgtggacct
      961 ccacggcggc accggcgagc ggacccatcg cccgcactcg atccaggaca tgcgcgaggg
     1021 ctaccggctc ggcatggggg acatgaccgt cgacctgcgc gacgtcaagt tccccccggg
     1081 cgaccgcaag ctcgacctgg agatcgggac cggccatatc gagctgatcg tgcccgcgga
     1141 cgtctgcgtc gtgaccaagg cgcgggtcgg cgcgggctac gtcggcgcgc tcgaccgcga
     1201 cagcggcggg ctcgacgtgg agtgggacga cgcgccggtg ccgccggcgg gcgtgccgcg
     1261 cctggtggtc gacgccgacg tcggcatcgg ggcgatcatg atcggcaacc gcccgatggg
     1321 cgacgacttc cagccgggac gcttcggctc taacgaggcg tgcagcaggc agccggcgcg
     1381 atgagccgcc gcgcgctcga tctcacctcg ctggtcgcgg gcgtcgcgat cgccgtgctc
     1441 ggcgcgctgc tgctgctcga ccaggaggac gcgatcgacc tcggcttcgc gtacctcgcc
     1501 cccgcggtca ctgcgaccgt aggggcgatc ctgcttgcca gcgggctgag ccgctcacgc
     1561 cgcggatgag cccggaccgc gtgggatcat cggacccgat gggcgctcca gcgagcccgg
     1621 cagcacccgc tcgccgcgcc gaggtgcggc gcgactcgca gcacggcatc gtcgccggcg
     1681 tcggcgcggg catcgcccgc gcgctcggcg tcgatcccgt gatcgtgcgc gtcgtcttcg
     1741 gggtggccgc ggtcgtcggc ggcatcggcc tgatcgccta cgcgctgggc tggctcctca
     1801 tccccgcctc cgatggtgga acgctggccg gccgcgtgct ctcccgccgc ggcagcgtgg
     1861 aggtggcgac cggcgccggg ctgctcggcc tgagcgcgat gctcctgctg cgcgcgctcg
     1921 ggtggtggtt cgacgacgcg gtcgcctggc ccgtcgtggc cgccgcggtc ggggtcgcgc
     1981 tgctctggca gcagtcggcg ggcgggtccg ccgaggccgc gacggcggac gagccggtgc
     2041 gcggcgagat ccggcgcgag ccgcttccgt tcctcggcgg gcgtgcgcgc agcaccgtcg
     2101 cgctcggcgc ggcgctcgtg ctcggcgcag ctctcgtgtt cctctgggcc aacggcgcgt
     2161 tcgacgcggc cggtgacgtg gtgctcgccg ccgtcgtggt cgtggtggcg ctcggcctga
     2221 tcctcgcgcc gctctggtgg cggctcggcc gcagcctcgc gaccgagcgg gccgagcgga
     2281 tcaggtccga ggaacgggcc gagctcgccg cccacctcca cgactccgtg ctccagaccc
     2341 tggccctggt ccagcgccgc gccgaccagc cgcgcgaggt ggcggcgctc gcgcggcgcc
     2401 aggagcgcga gctgcgggcc tggctgctgc gccggccgcc ggccggtgag ggcacgagcc
     2461 tctcggccgc gctcgaggag gccgccgcgg aggtcgagtc ggcgcacggt gccgcgatcg
     2521 aggtcgtgac ggtgggcgac tgcccgctcg acgcccgcat cgagccggta gtcgcggccg
     2581 cacgcgaggc gatgctgaac gcggcgaagt tcgcgggcgc gagcggtccg atcgacgcgt
     2641 tcgccgaggt gagcgccgac cgggtcgtgg tgttcgttcg cgaccgcggt gacggtttcg
     2701 atcctgccgc ggtcccggag gagcgccgtg gcgtacgcga gtcgatcgtc gggcggatgg
     2761 agcgcaacgg ggggagcgcg gtggtgcgat cgagcccggg cgcgggcacc gaggtggagc
     2821 tgtcggtcgc gaggggagcg ccgtgagcgc gatacgcgta gtgatcgtcg acgaccacgc
     2881 gatcttccgc gccggcgtgc gcagcgagct cggtgagcgg gtgaaggtgg tgggggaggc
     2941 gggcacggtc gcggacgccg tcgggtgcat cggcgacctc gagcccgacg tggtgctgct
     3001 cgacgtgcac atgcccgacg gcggcggggt cgaggtgatc cgccaggtga ccgccgcgga
     3061 cgagcccgcg gcgacccggt tcctcgcgct ttcggtcagc gacgccgccg aggacgtgat
     3121 cgccgtgatc cgcgccggcg cgcgcggcta cgtgacgaag acgatcgccg gctcggagct
     3181 ggccgaggcg atcgagcgcg tgcacggcgg cgatgccgtc ttctcgcccc ggctggccgg
     3241 cttcgtgctc gacgcgttcg ccggcgaggc gacgcccacc gaggccgatc ccgagctcga
     3301 tcagctcacc gcgcgcgagc gcgaggtcct ccagcacatc gcgcgtggat acatgtacaa
     3361 ggagatcgcc caccgcctcg gcatctccgc gaagaccgtc gaggcgcacg tctcggcggt
     3421 gcttcgcaag ctccagctat cgagccgcca cgagctgagc cggtgggcga tggacaggcg
     3481 gctggtggat ccgggcggcg agccctagtc gaggggcggg ccgtagcccg cccctcgaaa
     3541 ggcaatgctg cgtcgtgccg ggtctcagcc ccagagcatg acgtagaacg tctggtccgc
     3601 cgcggctccc gagctgttgt aagtcaggat ctgcgcgccg ctcgggcagt tgcccgtcgg
     3661 gcccttgctg aactgggcgg tcgagcccgc ggtgccggtg aactgggtga tgaccgacgc
     3721 gcccttgacg gcgaacgcga ggttgttcac gcagtagatg ccgttgttca cgcggacgat
     3781 gttcgcctgg atgatcccct tggcgtcgga gatgttgccg gtggcgccgt cgacggtcgc
     3841 gaacgccatc ggctgcgcgc tgtccgcggt ggtggcgttg gtcgcgttgg tggcgttgtc
     3901 ggcggccgac gccttgggaa ccgtgccgag ggtgccctcg ttcacgtcgt cgccggtcac
     3961 cgagtcgtcc cgcaggtcag cgccgacgag agccttgtcc ttcacgtcgc tcgaggcgac
     4021 ctgcccgttc ttgatcttcg cgctcgtcac cgagttgctc ttgagctgag ccgtgccgac
     4081 gctgttcttg ggaagcgtcg cggcccaggc gacgccgctc atcgccatga agacggcgat
     4141 gctcgacatc acgttcgcgt aggtgagctt cccccgaagt cgttgaagca tttgcgcgga
     4201 tcctcctccg tttgggttgt gtgggggcac cccaaccggc tacaggggat cttgccgggg
     4261 gtttggtgcc aagcgcgggg ggcgatcgcc cgcggctgac agcgcgcatc cggaaccgtg
     4321 cgtttccagc cagcggagcg gcgatggaat cgaaccatcc aagcgggggg ctgccccgcc
     4381 ctgccggttt tgaagaccgg atgggccacc agacccctgc cgctccaggt cgcggcagtc
     4441 taagtccgcg gcgcggccgc ttcacgcacc ccttcgcgcg agcggaccgg accatccgct
     4501 ccccctgtcc gcgctacccc ccaaacgggg gggagatacc tggaggcgca gagccccgtc
     4561 cagtgcggct cccgcttccc tcctgcccta gtcgccagcg tggcccgcta tcgaccaccc
     4621 cccaaatggg ggataacgct cgctaggccc cagccaccgg ctgcagcgac gtgtcttcgg
     4681 ccgggtcgtg cggcgcgccg gccgggaagc gcaggagctc cgtgcgcccg ccgcggtggt
     4741 cctcccagcg gcgcagcatc atcaccggga cgatcgccgc catcacgatc gcgccggtca
     4801 gaacgacctt cgctccccac tggctgccgc cgaccacgag cagcaccgcg ttgaaagccg
     4861 acagcgcggc cgccagcgcg acggccccgc ggccgaggcg gtagccgccg ccggccccga
     4921 tgtccccgct ggccccgctg accgctcccg cgggcccgtc gccgtcgccg accgcgtccc
     4981 caccgcccac cgcctccccc cgccgcagca cgatgtacgc gatcggcagc agcacgaaca
     5041 cgatcaggta gccgacgttg gccgaggcga ccacgtccac gacgtcggtc ccgaagacca
     5101 gcagcaccag caggatcacc accgcgtcgc agccgatcga gccgatcggc acgccccgcg
     5161 agttcaatcg cgcgaagaac cgcggcaggt agccgtcgcg ggtcatctgg taaatcgtgc
     5221 gcgacgagcc caccatgaac gcctgcgccc ccaggaccag cgcggcggtc agcatcaggc
     5281 cgatgatcgt gcggccggcg tcgccgaaga ccgactccgc gaccggcagg aagatggcca
     5341 tcgggtcctc ggtcagcccg ccgacgccga ccagcgcgag cacgaagaac ggcagggcac
     5401 cgaacacgac cacccccatc gacgccgcga tcttcatcgc gcgcggggcg tgctccgccg
     5461 gttcgcgcat ctcggccacc accgtcgagg ccatctccgc cccgtacgcg gcccacgcgg
     5521 ccacgaacat ccacttggcg atcaagagcc aggtggcccc cgccgtccac gagtggtcgg
     5581 ggaccgacag cggcgtcagc cgatcgaggt cgatcagcga cggctgcacg atcacgccga
     5641 tgacgagcgc gatcagcggc ccgaccgcaa ggagcgcggc cagcgccgcc gcgcgcatgc
     5701 tcgcccgcag cccgagcgcg ttcagcgcgt agagcacggc cccgatcacc agcgcgaagc
     5761 cgagcgtgct gatcccgctc cagaccgtgt cgcgcaggta ggtcgccgcc aggatcaggt
     5821 tcacggcgat gcccggcgtc catgcgaacc agtagcccca gctcgacgcc gctcccaaaa
     5881 tcggagcgcg ctcgcccacg gccgcgtgca cgtacgtcgc cgtgcccccc gcgcgtcccg
     5941 gcatccgcgc ggccagctcg gccagcagca ggcattgcag cgcaccgacg agcgcgatca
     6001 ccagccacac caccgccgag aagcgcccga ggtcctcagc catcggcccc agcgagaccg
     6061 tgatcatcag cgccgctccg agcgtgacca gcagcgacgt ccgccagctc accgtgcgcc
     6121 acagcatccc gtcgcggcgg gcgcgcagcg gcgactcgcg cccgccgggc gccggcgata
     6181 tccccgaccg tccccccgct gacgccccgt tcccctcgtc ccccaagcgc acgcccacca
     6241 gatccccccc cggccagggg ggctaacccc ccggattagt gactgggcgt cgagtattcc
     6301 accacacgcg cccctcgcaa actccggtaa cgtgccttcg acgcatgggc cgcggcgacg
     6361 catcgcacgc gcacgagcgc gggcacatca cggactcgcg tttccacgga cacatgtacg
     6421 cgacggaggc gagccgcgcg atcttctgcg acacgtgccg gctccagcgc tggctcgaca
     6481 tcgaggtcgc cctctccgcg agccaggccg agctcgggat gatccccgcc tccgccgcgg
     6541 agcggatcgc cgccgccgcg cgcatcgagc tgatcgacct ggacgcgctc caggccgaca
     6601 tccgccgcac ccaccactcg ctcgtcccgc tgatccgcgc gctccaggcc ggatgcgagg
     6661 agggcgcggg cgagttcgtc cactacggcg cgaccacgca ggacatccag gacaccggcc
     6721 aggcgctcga gatgcgcgac gccctcgacg agctgacccg cgtcggccag tcgatcgcgc
     6781 gcaccgtggt agcgctcgcg cgcgagcacg gggacgccgt gatcatcggt cgcacgcacg
     6841 cgcagcccgc gctgccgacc acgttcggcc tgaagctcgc aggctgggcc gacgagctgc
     6901 tccgcgacct cgagcggatc gaggccatgc gcccgcgcgt gttgacgatc cagctcttcg
     6961 gcggtgtggg cacgatgtcg gcctggggcg aggggggctc cgcgcttgtg gaacgggtgg
     7021 ccgaacgcct cgggctgagc gttccggcga tcgcctggca cgcgagccgc gatcgcgtgg
     7081 ccgagtacgc gagcacggtg gcgatggccg cggccacctt cgcgcgcatc gccgacgaga
     7141 tccgcacgct ctcacggccc gagttcggcg agctcgagct cggctggcac gagggccagg
     7201 tcgggagcag cacgatgccg cacaagcgca accccgagtc ggtccagcag gtcgtggccc
     7261 tggcgcgcct cgcggccggc caggtgccgg gcaccctgca cgcgatgatc gtcgagcacg
     7321 agcgcgactc ccgcacactg cggatggagt ggccgcttgt cgcggacgtg tcgcactacg
     7381 ccctcgccgc gggcgcgatc ctcgacacgg tgctgggcga gctggtcgtg cacccggagg
     7441 cgatgctgcg caacgccgag cgcacggccg cgctgctggc caccgagtcg ctgatgctcg
     7501 ccctcgccgg ccgcatgggc aagcagtcgg cctacaagct cgtgtacggc ctcagccagg
     7561 ccgcgaccag cgccggcagg cacgtgcgcg acgagctggc cgcagacgag caggtccgcg
     7621 agcacctgac cgagcaggag ctcgacgagg tcttcgaccc cgcgcgccac gtcggcgacg
     7681 cgcacgcgat ggtcgagcgc gcgctcgcgc acgcggagga gcgcctcggc gtcgcggacg
     7741 aggtcgcggt cgggtcatga gctcgagccc cgccgcggcc ggggcgttgg acctcgcgcg
     7801 gctccgcgag ctgcggctcg agcgcgagca ctacgacacc atcgtcgccc acgggcgtcg
     7861 caagctggag gggccctatc tcgacgagga gacgcacgag cgccaggcct tcgggctgct
     7921 ggccggccat atcgagggcg acgccgcgag gacgatccag gtcttccccc tgctgcgcaa
     7981 catgcgccac gacccgcgct tcaggccggc catggacgag acggtcaacg agctgggcgt
     8041 cccctcgacc accccgctcc cgcgccgcgg ctggatcgcg gacccgcgcg agctgcttca
     8101 cgcgcacgcc cgctgcgagg aggacggctc gctgatcttc gccaactacc acatgcacaa
     8161 ggtcggctgg gagcacgacc cgctgcgcga caccccgacc gcgctcgacg ccgcgctggc
     8221 cgagggccag ggcgtctgga cgctgatcct gtcgctggtg gacccggagc gcccgctgct
     8281 gcgcgccttc tacgagggca tgaaggaccg cgaggcccgg atcctcgtgg ccggcgcacc
     8341 gctctgagcg gtgccgctca gctctgggcg gcgacctcgc gctcggcggg ctcggccatc
     8401 tgcgcctcga tcagctccac ggagcgctcg cgctccggga tctcgccgtt gaagagcagg
     8461 ttgccgtcga ccgccagcac cggcaccgcc ttggccccct gagcgagcag gcggttgctg
     8521 agctcggccg gcaccgccga gaatccgagc gccccgccga ccccgtactt cacgaccgtc
     8581 gcgcggccct cgaactcctt ctcgaggtag gcgacgaagt cgtcctgctc ccccgcgtga
     8641 cagcagccgt cctcgatcaa cacgatctca tgcattccct gatcctcctt taagtggaga
     8701 gtgacatagc accggtcttc aggtcggggt ctgtcccgac ctccacgtcc agcttgcagg
     8761 tggcgattcc ctggcgcccg gccagctcgg ccatgcgccg cgcgtagtcg gccgacggcc
     8821 ggccgaccga cgtctcgcgc acgctctcct tcgcgagcgc gtacgtctcc gccaggcagg
     8881 cgaacgtctg gccgcggtcg ggcagccgga agtcctgctc gcaggagtag ccggggatcc
     8941 gcacggcgcc gccgcgggcc atgctcacgc cgtggcgggc gagctcctcg cgctccgagt
     9001 cctcgacgtt cggcggctgc gcgacgtcga tcaccaccgc gccgggccgc aggtgctccg
     9061 ggcgcagctt ggccgcgggg tcgttggtca gcgtgaccac gacgtcggcc tcgcccaccg
     9121 ggcccaggtc gccggccagc tcggcccgct cggcgagccc gccgagcacc ttcgccgcac
     9181 gcgcgggcga cgacccgatc aggacgaggt cgaagccgtc gtcggccagg agctccgaca
     9241 gcgggacgcc gaccgagccc gtggcgccga ccaccgcgac gcgcgtccgc tccggcgcgc
     9301 gctccccacg ggcctcgtgc acgttctggt gggcgatcgc ggcggtgagg ccgttgccgt
     9361 tcgtcacggt gacggtgggc gggacgtgcc gcaccaccgc ccgtccgccc gccgtggcgg
     9421 gcgaggtgag cgcgccgagc ccgacgaagt gcgacccgcg ctgcaccgcg agcttcacgg
     9481 cgtcgatcac cgcccggtgc ccgtccggcg tgacgatcgc ctccggcagc agcgggaccc
     9541 cgatgatctc gccgcggatc gccgccgtac cgaaccgcac gtcatcgagg acgaccggcg
     9601 ggttcgtcga gagcttctcg acgaactcct cgtcgctcga gctgtgctcg cggatgagcg
     9661 tcgccccggg gaacgtcagc aggtcgcgca ggtcgcgcgc gtgcacgacg aacgcgaacc
     9721 acgggacctg gttaccgagg aacaacggcg cccttcggcg cggccgtcgc tatgccatcc
     9781 ccagcgtggg gatgatcacg agcgagccct cgaacgtgac gtgtgtggcc tctccgccga
     9841 gatggtcagg ccacttcaac aggaaggtcg tgttgtagat cgagttgacg gaacgcccct
     9901 cgcggagcac ttccaggctg tcctccaaac tggaggacac gcgaccgtct cacccgcctc
     9961 cggcgccgga ccacatggag cccggcagga agtccattcg cccgcgggcg tgccacccgg
    10021 agtagtcctc gtagacgtcg cagtcgtcgg tcgcggtggc gcggatcgga tgcagcaggt
    10081 tgcggtcgta cttgccctcg gtgacgaggt cgtgcagccc cgggaagagg cactggacgt
    10141 ggtactggac gcgcagcttg cggttctcga agtccaccat cccgaagttg ctcgcggacg
    10201 gggcctgcgg atcgagcgtc atcgcgacct gcccggagtc gatcgactcg gtcttgctca
    10261 gcgggagcct gagaccctcg cgcgagatgc cgtagaggcc gccgatctgg aacgcgacct
    10321 tctgggttgg ctccggcaac tcccggcggg cctggatctc ctcgatcgtc gggctgatct
    10381 gctcggtcgc catgcactcc tccaaagggt cgtggaccgc taccggacca taccaccgct
    10441 tggggcagct tcaccaggcg gtcgcggcgc gctctccttg gtcgccacac ggtgcttcgg
    10501 gatcttgacg acgtcccacg cgaggcccag catctccatc gcccggatga ccatcgccga
    10561 ggggtcgagc tcgtaccagc gcagcccgtg gcgggccgac gtggggaagg cgtggtggtt
    10621 gttgtgccag gactcgccga acgtcaggag ggcgagccag ggcacgttgc gcgagcggtc
    10681 ggcggtgccg aagcgccggc tgccgaagca gtggcagatc gagttgaccg agtacgtgat
    10741 gtggttgacg gcggcgatgc gcacgacgcc gccccacagg acgccgcgca cgaagccctc
    10801 gaacgtgccg gtcagcgcca ggtccaggag gcccgcgagc agcacgccgg cgatcacgat
    10861 gtggatgaag tgcatgctga tccagcgcat ctcgcgctcg cggctcaggt ccggggcgta
    10921 gcgcagcggc tccgaggtga gccggtcgcc gagaagccag ccgacgtggg cgtgccacag
    10981 tcccctgagt gcggccccgt tcccgcttcc ggacaggtgc gggctgtgcg ggtcgcccgg
    11041 ctcgtcggac agcgcgtggt gcttgcggtg gtgggcgacc cagacgatcg ccgggccctc
    11101 ggcggcgaac gtcccgaacg tggcgagcgc cagccgaacc ggcttgtggg tcttgaacgc
    11161 acggtgggtc agcagccggt ggaagccgat cgtgacgccg aagccggaga tcgtgaacat
    11221 ggtcgcgaag acgatcgcgt cctcgagcgt catcacccgc ggccagaacg cgaccaccgc
    11281 gaccagcagc gcgagcggcg ggacgagcac cgtcgcgagc atgtagcggc ggtaggcctg
    11341 gttcgacatc agccggcctg gagcctgtcg gcctggccgt tgacggcggc cagcatctcg
    11401 tcgagggtct ggacgtcggc gacgacggtg gcgtccagct cgtgccccac cagctcctcg
    11461 agctcgaaga acgtatccgc cacctcgaga ctcgacaggc cgaggtcgtc gaggcgcgag
    11521 ctgccgcgta tctcggtcct cgccccgcgc gacccgagct tggaccggat gatcgccgtg
    11581 gcttcgtcga tggtcacggt ccgcggcatc cggcgcacct tagcgttaag agcatgtcgg
    11641 gccggggcga gcgcgggggc gcccgaacgc gcgctgtaac atcggcttgc ggcccgtttg
    11701 cgagccggca gaggcacgat gagcactgga cccacagccc agaacgacgc cccggcggcc
    11761 tgctccctgg acgcggccgg cgccgcccgc cagctcgaca ccttcgcggc gttgtgcgag
    11821 cgcgcgctgg tggacttcga gcggggcgac acgagcgtcg cgttccggtt ccgtgacgag
    11881 gaggacgtgc ggcgctcgat cgacgagtac gttggcgtcg agcgcggctg ctgtggcttc
    11941 ctcgacttca cggtcgagga ccgcgaggga ctgcccgtcc tgaggatcgt cgccggcgag
    12001 ggcaccccgg ccgccgtcct gcactcgatg tacgggcctc ccggccgcgc gtgacccggg
    12061 atgaccgcgg ctccgggcga cggctccacc gtcgtccaca cctatagcga ccccgaggag
    12121 gggttcgagg gctacctcgc ctactcgggc gagggccggc cgctggccgc cggcgggttc
    12181 cgggtgcagc gcggtctctc ggcgcgcacg atccgtgagc tggcagaggc gatgaagctg
    12241 aaggaggggg tcctccagct caacgtggac ggggccaagt gcggcatcga ctacgacccc
    12301 ggctcctccg gcaagcacgc agcgatgagg cggttcctgc gcttcctggc gccgcacctc
    12361 gccggccggc tcagcctcgg gcccgacatg ggcaccacct tcccggagat agaggcgctc
    12421 gcgcgcgagg aggggatcgc ctcggtcaag ggggcgatcg cccgcgccca agggctgccg
    12481 gaggagcagg tgctgcggcg gctcgcgacg ctcgacctcg actccggtcc gctcaccgtc
    12541 ggctcgcgcc gcgccggcca cgcgctcgcg cacgccacct tcgccgccat gcgcagcgtg
    12601 ggcctgtcga gcccgccggc gacctgtgcc ctcgagggat tcggcacgct cggccgcggc
    12661 gccgcgctga cgctgcacga ggcggggatg gtcgtgaccg cggtggcgga cgagcacggc
    12721 tgcatcagcc gcgccggcgg actcgacctg gacgagatgc tgcgctcgcc ccacgcgacg
    12781 cccgttccgg agctggcccc gcccggggcc gccgtcgagg acagcgccgc ggtgctcgcg
    12841 ctccccgccg acgtgctcgt gctcgccgcg cgcgaggacg cgctcagcga ggaggacgtc
    12901 gagcgcgtcc aggcccgcgt ggtcgtggtc ggcgccaaca acgggctgcg cccgtccgtc
    12961 gaggagttcc tgttcgagcg cggtgtgctc gtggtgccgg acttcgtggg cggctccggc
    13021 ggctccgcct cgatggacgc tctgttcggg ccgccgctct gcccggaccc ggtcacggtg
    13081 ctcacgcgcg tcgcgatgac cgtgcgggcg ctggtcgagg acatcgccgc gacctccgag
    13141 cggatgcagc tctcgccgcg caccgccgcg ctcgccctgg cggcggatca cccgctgtcg
    13201 ccggacgcga agccctacgg catgcggctc ctgcgcgaca tcgagcgcgt ggacgcgctc
    13261 agcgcctccg acgtgcgcga ccgcgccagc cgcgtcgccg ccggctgcgc atggcaggcc
    13321 cagctcgagc acgccgggct cgagtagccg ctagagctgg cgctggaaga gccggacgag
    13381 gtcgcggacg gtctcgacca tgccgagctc gtcctcgggc acgcgcacgt cgagatcgtc
    13441 ctcgatctcc gcgacgacgt tcatcacggt catcgactcg acgtcgaggt cggcgatcgt
    13501 cgcgtggaga gtcaccgcct cgatggccgc gtccgggtcg atcacggcca ggtggtcgcg
    13561 gatgcgcgcg aggatcgcgg cgtcggagga gtcggtcatc gcgccgccgc cggctcgagg
    13621 gcgccggcga ggtagcggct gcgcagctcg ccgcggcgca gcttgccgct cgtcgtcttc
    13681 ggcagcgtcc cccgctcgac cacgagcacg tcggcggctc tcacgcctag ctggtcgagc
    13741 acgcgcttgc ggatcgcgtc ggcgagctcg ccgtggccgc ggaagcggtc ggtctcggcc
    13801 acgatcaccg ctccctccgc gccgtcgctc gccgggcacg agaacgcgac caccccggcg
    13861 acgcgcacgt cgtcgagctc cgcggcgcac cactcgaggt cctggggatg gtggttgcgc
    13921 ccgcgcagca cgagcaggtc cttcagccgc ccggtcacga acagctcgcc gccgtcgagg
    13981 aagcccaggt cgcccgtcac gacggagccg tcgcggaacg tctcggcgct gcgctcgggg
    14041 tcgcggtagt agcccttggc ggcgctcggg ccgcggacga cgatcgagcc ctcgcggccc
    14101 tcggggagcg gtgccccgtc gtcgcccacc acggtcacct cgtggcccgc cagcgcgggc
    14161 ccgcagctca ccggctcgac cccctccgcg cccgcgtcca gtgtgcgcat gccgcggccg
    14221 ggcggggtgg cggtcacgac catcgtcgac tccgcgagtc cgtacgacgg cagcagggca
    14281 tcgggcgaga agcccgcggg ctccaggcgc ttcgcgaagc ggcgcagcga gtcggcgcgg
    14341 atcggctcgg cgccgcagcc cgccacgcgc cacgacgaca ggtcccaccc ggccaggtcc
    14401 tcgtcgcgca cgcgctccgc gacgatgtcg tagccgaagt tcggtgcgta ggtcaccgtc
    14461 ccgccgaccc tgctcatcag cgcgcaccag tccggggggc ggcgcgcgaa cgcgagcggg
    14521 gacatgaacc aggtcggggt ctcctccagc aggggagtga tgaccatccc gacaaggccc
    14581 atgtcgtgga acatcgggag ccagctcacg acgcggtcgc ggtcgccgtg gatctcgagg
    14641 tgagcgctga tcgcgcgtgc gttcgcaacc acggcgtcgt gggtgatcat cacccccttc
    14701 gggtcggccg tgctgccgga tgtgaactga aggaacgcga tctcgccgcg gggctcggcg
    14761 gggaggtcgg cggcgggacg catggcctcg aggtcctcga agctaaacga cgggaccgcg
    14821 ttgggcgcga gccgcgccgc gaccgccggc aacgtgatga cggccgccgc ctcggacacg
    14881 cgcatgaccg cgcggatgcg gtccgcgtag gcctccatgc gctggcccgc gaacggcggg
    14941 tacaggggga cgggcacgac gcccgccagc accgcgccga ggaacacctg cacgaactcc
    15001 tcctcgctgg ggaggatcag caccgcgcgg tcgccgggtg cgagccccgc gccgcgcagc
    15061 cgccgcgcca tcgcgcgggc ccgctcgcgc agctcggcga acgcgatcgg tcgctcggta
    15121 cgcccggcga ggaaggtgaa cccgcggtcc tcgtgaccga gcgcctcgac cacgtcggcc
    15181 accgttgcga cgctctcgcc gtccacctca gccctcgccc cctcgcgctc gacccgcttc
    15241 ctacggtatg ccataccgca ctgcgcgcgg ttatattccc gcgcctgacg acccggccca
    15301 cggctctcct cgaagcatgt cggtgatcga gacccgcgat ctgtcgcggc gcttcgccgg
    15361 aggagtcgcc gccgtcgacg ggctcacgat gcgtgtcgag gccggcgaga tcttcgcgtt
    15421 cctcggaccc aacggggccg gcaagaccac cacgatccgc atcctcgtga cgctgttgaa
    15481 gccgaccggc ggtgaggcga cggtggccgg ccacgacgtg gtgcgcgagg ccgggcgcgt
    15541 gcgcgagaag atcggaatcg cgcttcagga ggccgccctc gacccgctca tgaccggcgt
    15601 cgagtgcctc gacctccagg cgtccctcta cggggttccg gacgcgaccg cccgccagcg
    15661 ccgcgacgaa ctgctcgaga gcgtcgagct cgtggatgcc gcgagccggc gagtcggcac
    15721 ctactcgggc gggatgcgcc gccggctcga cctggcgatg gcgctcatcc accgcccgga
    15781 ggtgctcttc ctcgacgagc cgacgacggg cctcgacccc ggcagccgcc agacgatctg
    15841 ggagcgggtg cgcgagcttg cggccgccgg caccacgatc ttcctgacca cgcagtacct
    15901 cgaggaggcc gaccggctgg ccgggcgggt gatgatcatc gacagcggca acgcgattgc
    15961 cgaggggacg cccgcccagc tcaaggcgga gttcggcgcg ccgcggatcg agatcacgat
    16021 cgaggagcgg gtcgccgagg cggtgctgcc cagactggcg cgcttcggga ccgcgcgggt
    16081 cgagcgcccc ggggtgatct cgctcgccac ggagggggag ctgccgtcgg tcggcgcgat
    16141 cgccggcgcg ttcgagggag agagcgcgtg cgtgcgctcg atcgaggtgg tcgagccgac
    16201 gctcgacgac gtgttcctcg ccaggaccgg tcaccagttg aaggagtccg ccgccgaggg
    16261 agcggccccg ccggatgagc ggcgcgaagc gcaacttccg ggcgggtagc cggctcgccc
    16321 gcagggcgac cgcccagacg ctgcgccggc cgccgctggt cggcccgatc ctgatcctgc
    16381 cgaccgtcgt gctgatcgcg ttctccggag ggggcgccag cgcggtcgac ctgcccgggt
    16441 tcccggaggt gccgagcttc ttcgacttcg agcttccggg gatagtcacg atgtcggccg
    16501 cgctcgccgg ggtgagcggc gcgctggccc tggccggcga cctcgagatg acgttcatgg
    16561 accgcgtgct ggcggcgccg ctgtggcggg gcgtgcccgt ggtcgggcgc ctggccggct
    16621 cggccgcgct ggccgtcgtg tcctgcgtgt ggttcttcac gctcggcctg atcttcggag
    16681 actggccgtc ggagggcgtg ctcgggctgc tcgtcatagc cgggctcgcc gcgctcacgg
    16741 cggccgcggt cgcgtcggtg atcgcgacca tcgcaatacg cacgcgccag tcgagcgtga
    16801 tccaggggtt cttccccctg atgctgttct tcctgctgct ctcgtcggcg tacttcccac
    16861 gccacctgct gcacgagccc gcgcggacga tcgcgggctt caacccgctc accgccgtcg
    16921 ccgacgcgat gcgcgacgcc tggatcggcg agctcagcgt cggcgcgacc gtcggagcgg
    16981 ctggcgcggt ggccgcgctg gcactcgtcg gggccgtcgg ctggggcctg gcgctcgtcg
    17041 ggacacgcag gtgaggccgg tcggggtcaa gcggctcgtg cgactgctgc ggcggtcgct
    17101 gaacgaggtg gtgcgcgtgc cgggcgtgac ggtgcagaac gtgctcgcgc cgacgctgat
    17161 catgctcggc acgacggcgg tcttcggcac gctcgtggag ggcggcggct tcgggaccga
    17221 cgactacatg acgttcctgc tcccctgggg cttcctccag ggcgcggcct tcaccggcgc
    17281 cgcgacgggc gtgaacttcg cgcgcgacat cgagcgcggg ttcgtggacc gcctcctggt
    17341 ggcgccgatc gcgcgcgggc tcctgatcgt ggtgaacgcc ctgtcggcgg ctgtgcgcac
    17401 ggtgctgccg ctggcgctgc tcgtcccggt ggcgctcgcc ttcggggcgg agctgtccgg
    17461 gccgggcggg gtggccgtgg cggcgctcgt cgcggccggg ttcgcgatgc tcgcctcggc
    17521 ctgggggacg ttcgtcgcgc tcgtgacccg gacgcaggag gccggtccga tgatgcaggc
    17581 cccggtgctg ctcacgatca tgctcaccac ctcgttcgct ccggaggagc ggctcgcggg
    17641 atggatgcgc gacatcgccg acgtgaaccc cgtcacgccg atcctcgagc tcgcgcggca
    17701 ggcgatcctc tccgacgtcc tctggtccga cgtgcgcccg gcgctgatcg ccttcgctgg
    17761 gatcggcgtc gtgctctacg cgctcacctg gaaccagatg cggcgcgcgc tcgtataggg
    17821 tcggctcgtg aagcccattc tctacgtcat cccggggacg catccgtgca tgaccgcgcg
    17881 gctcatgctc gagcacaagg gcatcgacta ccggcgcatc gatctgctgc cgcccatgca
    17941 ccgcccgttc ctcagggcgc tgcggttccc ggacgagacc gtccccgccc tgcacatcgg
    18001 cggggccaag atcacgaaca cgcgcaggat ctcgcgtgcg ctggaggagc gggtaccgaa
    18061 cccgccgctg ttcccgagcg accccgagcg gcgcgctgcc gtcgaggagg ccgagcgctg
    18121 gggcgaccag gagttctggg agggccccaa ccggatcgcc tggtgggcgg tcgcgcacga
    18181 ccagccgtcg atcccgagcc tgatggagga ggcccgcgtt ccgatccggc tcgcgccggt
    18241 cctggcgccg ccgatcgcga ggatgggcgt gaagcgcaac agcgccgacg acgagcacac
    18301 gcggccggac atcgaggcgt tgcccgccca gctcgacaag atcgaggcgt ggatcgacga
    18361 gggcgtgctc ggaggggacg agctcaacgc ggccgactac cagatcgccc cgagcctgag
    18421 gatcatgatg tgcctcgacg acctgcgccc gctgttcgac gcccgcccgg tgggcccgta
    18481 cgcgcgccgg gccgcgccgt acttcccggg acagatcaag ccggtggtgc cggaggagtg
    18541 gctggctccg ctgcgcgagg ccgctccggc cgccgccggc tgacgctccc ggcctccgcc
    18601 ggctgacgct cccggcctcc cgccggctga cgctcccggc ttcccgccgg ctgacgcctc
    18661 ctttccattt ctcttcgcgc gcgcacgcgc gcgcgaggga ccggacgggt ctgcgcccgc
    18721 ccggcccgca cgctccggcc gaggtcggct ctacgcggcc acgcgatcgg tcagcagttc
    18781 accaccaggt ggaacggccg gtccgcccgc gccgtcggct cggtgccgtc ggccccaccg
    18841 cccgcgcgcg tcacgacggt gaccgcgttc gggctgccgc tcacgggtgc ggccgcggcg
    18901 gcgccggcat cggtcgttgt ggcctctgtc gcgctgagag cgcacttcga gacgtcgctc
    18961 gcgaacgtca ccgtgtacgt accggcgccc gtcttctgga ccgccgcggc ctgggcgagg
    19021 ccgcggccgg tcgcctcggt cccggcggcg ttcaccatcg cgagctttgg ctgggaggcg
    19081 gcgaccgcat cgctcacgag gtcggccgcg ctcttgccgt cgacctcgtc ggcgttcagc
    19141 cctgtcgcga cgccggtggc gttcgtggtg aacggccgca cgtcgtcggc cgtcttgccc
    19201 gccggcggga tcacctcgaa gaaaccggcg ctctcgcccc cgcggctgcg gaagtagaac
    19261 gcctcgccgc cgcggtggtt cagcacgaac aggcacggct cggcggcgag cggcgcccgg
    19321 cagccgtagc tcgcggctcc gccgtcgccc tcctggttgt tcgactggcg ggtggcgtag
    19381 gtcgtgctcg taccgaccat cgccgtctgg ccgagggccg cgttgcgctt gccgatcagc
    19441 acggggtcgc cgaccgcgcc ggtggcgacc gaggtcacgc cgagggcgag cgcggtcgcg
    19501 caggtcagga tccagactgg cttcattggt gcctcctggg attcgggtcg tggggactcg
    19561 aacccggtag ccggggtcgc gctgcggggc tgcaagccgg gtcgcaggat cgtgtcgccg
    19621 tgctgacagc ggggtgcggc tccgggcggc ggataccctc ttcggggatg tcgacgcaac
    19681 ccaaccagcg cagcgtcaag atcgccgacg acgagcgcgg cggctggtcc tcggaccgcc
    19741 cacgcaaggg tccgccgcgc gaggcggaca tgtcggtccg ctgccgggtg ctcaagccgg
    19801 agccgcagat gcgctactcg cccgggagcc tgctgatggt cgtgtcgggc tcgcccgagg
    19861 agcgcgatcg cttcaccgct cgcgtgctcg aggggccggg gctgttgctg tcgctcgaca
    19921 aggtgcgcgg gctgctgacc gggcgcggtg ccgcggagga cgtggccgcc gagcgtgcac
    19981 ccgagctgct cgacaccgcc gtgatgaagc gactcgaggc gaacgagacc gtggtggtcg
    20041 ccgccgacgg gctcgaggcg ggggagcgcg agcgcttcgt gcgtatggcc gcgcagcacc
    20101 gccgcccgcg ccacctgatc ctcatcgagg ccgcccgcga ctccgtctcc gacgaggccc
    20161 gcccggccct gaacgacctg cgccggcgcc tcgacgcggg ggagctcggc gccgagggct
    20221 tccagaccgc cctgcgcctg ggcggccagt cggtcggcga gctcaagcgg atcgtcttcc
    20281 gccccgagcc ccgcgacgac tagcgcctag cgcgcgcggc ctccgcgcgc cccgcccacg
    20341 acgtcctggg ccatattgac cccgctatac ggggcaaaga tggcccagtt gcccgaacca
    20401 ccgcggacag cgccgcccac gccgccgtcc ttgggagaac ccgcgcggtc tccttgccaa
    20461 ctccttagcc gcggcttgcc ggtcgctgac ggcggcctcc gatgctctgg acgtccagtc
    20521 gatttcccga cgaggagtcc ttccagatgc ccggcatccg caccacgatc gcaaccgcag
    20581 gaacgctcgc cgcactcggc gcgctgaccg cgttcgcgtc cagctccggc cagaagcccg
    20641 agtcccagcc cgccaagtcc agcgcggtgc cgaccgaggt ccgcaccaag gtcatcaccc
    20701 gcactatccg ccggcgcgag cgcgcgtcgg gtggcggtgg ctcggcggct ccgcggtccg
    20761 ggggcggcgg cggaggcggc ggcggtgccc ggtccgctgg cccggctccg cctgcaccga
    20821 tttcgcgcgc cgcggtgtcc gcaccgccgg tgaccgcccc gagcggcggc ccctccagct
    20881 ccggcgccgg ccagtccggc ggcggcgacg atcacggccg cggccgtggc cgcggtcgcg
    20941 gcggcgacga ggaccgctcc ggccacggcg gtggcggtgg cggcgacgac cactccggcc
    21001 acggcggtgg cggccacggg ggctccggcg atgactgagc ccaccctgac cgacgcaccg
    21061 gccccggcgc cggagacgcg cgtggcggag cacgcgccga ctgttgcgct tccgcccgcg
    21121 gcggagcccg ccgtgcccgc ggcggcccag gcggcggagc ccgtcgagcc agcagccgcc
    21181 gctgcggcgg aggctccgcc cgcggccgcc gctccggcgg cggagcccgt cgagcccgcg
    21241 gccgccgctc cggcggcg
//
LOCUS       DASVPL010000291         6164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_34128, whole genome shotgun sequence.
ACCESSION   DASVPL010000291 DASVPL010000000
VERSION     DASVPL010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34128"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(145..273)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            362..434
                     /locus_tag="VF517_16305"
     tRNA            362..434
                     /locus_tag="VF517_16305"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:396..398,aa:Glu,seq:ctc)
     gene            537..869
                     /locus_tag="VF517_16310"
     CDS             537..869
                     /locus_tag="VF517_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054551.1"
                     /translation="MLDEVEWSGDGVTALVLLVLGLTFVAFGFAWGRSRAITVVAFVL
                     TVASVGLDALYFLFDVNLAGYCEEPKCDPGPIPMSIMLIVLPVPLALTYIGVHVRRVR
                     REARSEGA"
     gene            923..1327
                     /locus_tag="VF517_16315"
     CDS             923..1327
                     /locus_tag="VF517_16315"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8054552.1"
                     /translation="MPFTLKNLKEDLEDLGSNFDGPPDLEFRHASKALGLERSGLSYQ
                     RIPPGYRFPYGHTHREQEEVYVVVRGSGRMKLDDEIVEVREWDVVRVPPGTWRGYEAG
                     PEGLEILVIGAPSLGENPREDVEGRRDWWAGE"
     gene            1401..2063
                     /locus_tag="VF517_16320"
     CDS             1401..2063
                     /locus_tag="VF517_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054553.1"
                     /translation="MTRKALVAALAVTATMLLAPAAQAKHRSASCYPHGSRTVTANNF
                     VRVYGVPPTDPDAQGSRRLVACSLTSGRRKALGGYEACQFAGQYLFNIRLTGRMVGYV
                     SAECLGFSSSVYVRDMTTGRVLWIAETAPGRGHTVTDLEVARNGSAAWIAAWKNAEYE
                     RDYEGRVLRTVEVSGDRQPVTRDDSLDIKPGSLALGRTDGSGATPFYWSKGGQVFSAT
                     LR"
     gene            complement(2081..2779)
                     /locus_tag="VF517_16325"
     CDS             complement(2081..2779)
                     /locus_tag="VF517_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054554.1"
                     /translation="MLEHKGLPYRCVDIVPLTHPFVARLHGFDAGSQKRTAGDRRSVA
                     IRFGDRLGTVPGLAAGDERISTNHAIARFLEDRHPEPPLFPDDPARREAVEEVERWGN
                     DEFQMAARRIGLAWAAREPAQASRSVADGRMGPVLYRHQLTRRLMIPLIGRLVFNAGP
                     GLVEELLAGLPQMLDRIDAWIADGVLGGEQLNAADFMVAPSLALILYRPDVMHLFEGR
                     PALELVDRLLPEPA"
     gene            2866..3558
                     /locus_tag="VF517_16330"
     CDS             2866..3558
                     /locus_tag="VF517_16330"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054555.1"
                     /translation="MAQTSPHSRGRPGAKDSTRLDQRTRAARAQGRGAREELLAAALR
                     VFARRGYTQAGVDEIAAEAGYSKGALYWHFAGKEDLLLSLLDERIDAPMRSMLALLET
                     APPERDMSLEANGVFARLMGGQRDAVLLEREYWSLAIRDPELRARYAERQTEFRTVFA
                     RALEARARRLGTPDLPMDSEDVARIVMGVFAGLAVDELIEPGSVSPELLGEALALIYA
                     GLIARAAPPASA"
     gene            3588..3914
                     /locus_tag="VF517_16335"
     CDS             3588..3914
                     /locus_tag="VF517_16335"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX8054556.1"
                     /translation="MVPSETERLLRARLDELQARIAGLAKTPEPGSGIGFGKRVGDGT
                     TEAISRRNEIGIGTSLEVSEQRLLRALAKVEEGSYGVCDRCGEQIAPARLEVAPESVL
                     CIGCAR"
     gene            complement(3918..5477)
                     /locus_tag="VF517_16340"
     CDS             complement(3918..5477)
                     /locus_tag="VF517_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054557.1"
                     /translation="MGAARRGGWLAIALALLAAGPAAAQDERDCFWQGGTRSTNIAYP
                     DLGARYWVSSFPLPPGAELILRGRYPHARYMSFNVYDAAAQPTDGLADIDIAPDPGAA
                     NPFPPGAQRDLERREYTVRVVGGARPEKREPNTIYLNAAGQGTPVGVIMYRVYVPDAG
                     RDPAGGVGLAEVSMRLPDGQVLDQPLVCQARADTGANQAVEAFHSGTDGPDSSNPTSE
                     DPIRWEAFFNYFQAFSYPAGATPAGSGRAVLPRERLGGFLSNIDNAYTLATANRGIGP
                     VAVLEGLAPTTPRTLDGRRVMEGGQVRYWSLCENEFASQRVIDCVFDEQVRTRADGRF
                     TLVMSEPGERPANARPECGVNWIAWGAQPDGLLILRHMLPSPDFAQAIQRVAELGTEQ
                     GVIGEYLPVGRHTTRADFEARGCPAPEAAASQPRTAPRPAIRLSVSPAQARAGRRVAF
                     RFRARYRAAGRLLPVVGARVRFANKVGVTDSRGRVTIVQLFTHARAYRPRACRRGFEC
                     GRVAVRALARG"
     gene            complement(5513..6046)
                     /locus_tag="VF517_16345"
     CDS             complement(5513..6046)
                     /locus_tag="VF517_16345"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX8054558.1"
                     /translation="MPRHIALLRAINLGSRNRVSMPELRELLAGHGYGDVKTLVQSGN
                     IVLTSRLSPARLERDLQRQIAEGLGVDTPVVVRTRDELAQVIERDPFGSDVDNPKLYQ
                     VTFLSEEPSDELAQELAEMDVAPERIALIGRELYTWHPDGIQRSKVDRLRTSKRMPTG
                     TARNWTTVTGLLALADE"
BASE COUNT          847 a         2238 c         2201 g          878 t
ORIGIN      
        1 ccacgcgcac gttgaccttg accgcgtcgg ccgtggccgg gacagccgcg agcgtgccga
       61 gtgcggccgc gacggcgaga gtacggagct tcatctgatt agcctcccct tccgcgaggg
      121 tgttgtggct tccggccgga gacgggtctc ctggctcccg gttatggccc ccatccgcgc
      181 cttcccaggg agcgggagct ccccagtggc ctcgtgcggc gggcctagcc ggtcacagtg
      241 gcgggaccgc gccggattct caccggactt ccctcgacac cggccgctta cgggggtgag
      301 cataggcgac ggacgcccgc ccggccaagc cgcggacgcc gcctataatc cgcgcccgct
      361 ggccgccatc gtctagggga ctaggacgcc gccctctcac ggcggaaacc ggggttcgaa
      421 tccccgtggc ggtacttgct ggagcccgcc ttcccgcgac cgcgaggggt tggcttgcga
      481 cggcaacgac ggctgccggg cttgcgatca gcgtcacggc tttttacttt gcgggggtgc
      541 tcgacgaggt cgagtggagc ggcgacgggg tcactgcgct cgttctcttg gttctcggct
      601 tgaccttcgt cgccttcggc ttcgcctggg gacggtcgcg agctatcacg gtcgtcgcgt
      661 tcgttctgac ggtggcctcc gtcggcctcg acgcgctcta tttcctcttc gacgtgaacc
      721 tcgccggcta ctgcgaggag cccaaatgcg atccggggcc gatcccgatg agcatcatgc
      781 tgatcgtcct gccggtgcct ctcgcgctga cctacatcgg cgtgcatgtt cgccgcgtcc
      841 gtcgcgaggc ccgaagcgag ggcgcgtgag gatcgcccga ggttcgcggg agcgtttcgc
      901 cgtggcgtcc taggatcgcg ccgtgccctt cacgctcaag aacctcaagg aggatctgga
      961 ggacctcgga tccaacttcg acggcccgcc ggacctcgag ttccgccacg cgagcaaggc
     1021 gctcgggctc gagcggtccg gcctcagcta tcagcgcatc ccgcccggct atcgcttccc
     1081 ctacggccac acgcacaggg agcaggagga ggtgtacgtg gtggtgcgtg ggagcgggcg
     1141 gatgaagctc gacgacgaga tcgtcgaggt cagggagtgg gacgtggtgc gcgtcccacc
     1201 cggtacgtgg cgaggctacg aggccgggcc ggagggcctc gagatcctcg tcatcggcgc
     1261 gcccagtctc ggcgagaatc cacgcgaaga cgtggagggt cggcgcgact ggtgggctgg
     1321 cgagtagccc ggcgatcccc gatacggagg accccctgta gcctcgcggc cgcaaggtaa
     1381 ctccacggag ggatgctcga atgactcgca aggccctggt ggcagcgctc gccgtcaccg
     1441 cgacgatgct gctcgcaccc gcagcgcagg cgaagcaccg ctcggcctcc tgctacccgc
     1501 acgggtcgcg gacagtgacg gccaacaact tcgtgcgcgt gtatggagtg ccgccgacgg
     1561 accccgacgc gcaggggagc cgccgtctcg tcgcgtgctc actcacgagt ggtcggcgga
     1621 aggcgctcgg cgggtacgag gcctgccagt tcgcgggtca gtacctcttc aacattcgcc
     1681 tcaccggccg gatggtcggc tacgtctccg cggagtgcct cggcttctcg tcatcggtct
     1741 acgtccgcga catgacgacc ggccgcgtgc tctggatcgc ggagacggca ccgggccgcg
     1801 gacacaccgt tacggacctg gaggtcgcgc gcaacgggtc ggcggcctgg atcgcagcct
     1861 ggaagaacgc cgagtacgag cgcgattacg agggccgcgt gctcaggacg gtggaggtct
     1921 ccggcgaccg ccaacccgtc acgcgcgacg acagcctcga catcaagccc gggtcgctcg
     1981 cactggggcg caccgacggg agcggagcga cgcccttcta ctggtcgaag ggcgggcagg
     2041 tcttctcggc gacgctgcgg taggcgtcag ccgcgggccc ctacgcgggc tccggcagca
     2101 gccggtccac gagctccagg gccggccggc cctcgaacag atgcatcacg tcgggccggt
     2161 agaggatcag cgcgagactc ggcgcgacca tgaagtccgc cgcgttgagc tgctccccgc
     2221 cgaggacgcc gtcggcgatc cacgcgtcga tccggtcgag catctggggc aggccggcga
     2281 gtagctcctc gaccaggccg gggccggcgt tgaacacgag gcggccgatc agcgggatca
     2341 tcaggcgccg cgtgagctgg tggcggtaga ggacggggcc catccggccg tctgccaccg
     2401 aacggcttgc ctgcgccggc tcgcgcgctg cccacgcgag gccgatgcgg cgggcggcca
     2461 tctggaactc gtcgttcccc cagcgctcga cctcctccac cgcctcccgc cgcgcgggat
     2521 cgtcagggaa cagcggcggc tccgggtggc gatcctccag gaagcgcgcg atcgcgtggt
     2581 tggtcgagat ccgctcgtcg ccggcggcga gacccggcac cgtgccgagg cgatcgccga
     2641 agcggatcgc gaccgagcgg cggtcgccgg cggtgcgctt ttgcgacccc gcgtcgaacc
     2701 cgtgcagccg ggccacaaag ggatgggtga gcggcacgat gtcgacgcat cggtacggca
     2761 gccccttgtg ctcgagcatg agcatcgccg agcgacaggc atgtgaagcg gggatcacgt
     2821 agagcttgta catactgacc ggtcagtcgg taccctaccg gaggcatggc tcaaacgtca
     2881 ccgcatagcc gcggccgccc cggcgctaag gacagcaccc gcctcgacca acgcacccgc
     2941 gccgcgcgcg ctcagggccg cggggctcgc gaggagctgc tggcggcggc gctgcgtgtc
     3001 ttcgcccgcc gcggctacac gcaggcgggc gtcgacgaga tcgccgccga ggcgggctac
     3061 tcgaaggggg cgctctactg gcacttcgcc ggcaaggagg acctcctcct gtcgctgctc
     3121 gacgagcgca tcgacgcgcc gatgcgctcg atgctcgcgc tactcgagac ggcgcccccc
     3181 gagcgcgaca tgtcgctgga ggccaacggc gtgttcgccc ggctgatggg cgggcagcgc
     3241 gacgccgtgc tgctcgagcg cgagtactgg agcctcgcga tccgggatcc cgagctccgc
     3301 gcccgctacg cggagcgcca gaccgagttc cgcaccgtct tcgcacgcgc gctcgaggca
     3361 cgcgcgcgcc gtctgggcac gcccgacctt cccatggaca gtgaggacgt ggcccggatc
     3421 gtgatgggcg tcttcgccgg cctcgcggtc gacgagctga tcgagcccgg gtcggtctcc
     3481 cccgagctgc tcggcgaggc gctcgccctc atctacgccg gactgatcgc gcgcgcggcg
     3541 ccgccggcca gcgcctgact cggcggtcgg gcgactacgc tcgcgcaatg gtcccgagtg
     3601 agaccgagcg cctcctccgc gcccgcctcg acgagctcca ggcccgcatt gccggcctgg
     3661 ccaagacgcc cgagccgggg tcaggcatcg ggttcggcaa gcgcgtcggc gacggcacga
     3721 ccgaggcgat cagccgtcgc aacgagatcg ggatcggcac cagcctggag gtgtcggagc
     3781 agcggctcct gcgcgcgctc gcgaaggtcg aggagggcag ctacggggtc tgcgaccgct
     3841 gcggagagca gatcgcgccc gcgcggctcg aggtggcccc cgagtcggtg ctctgcatcg
     3901 gctgcgcgcg ctaggcgcta cccccgcgcc agcgcccgaa ccgcgacccg accgcactcg
     3961 aacccgcgcc ggcacgcgcg cggcctgtac gcccgcgcgt gcgtgaagag ctgcacgatc
     4021 gtgacccggc cgcgcgagtc ggtcacgccg accttatttg cgaaccggac gcgcgcaccc
     4081 accaccggca gcagccgtcc cgcggcccgg tagcgggcgc ggaagcggaa cgccacgcgc
     4141 cgccccgccc gcgcctgcgc gggcgacacg ctgagccgga tcgccggccg tggcgccgtt
     4201 cgcggctgcg acgccgcggc ctccggcgcc gggcatccgc gcgcctcgaa gtcggccctc
     4261 gtcgtgtgcc gcccgaccgg caggtactcg ccgatcaccc cctgctcggt gccgagctcc
     4321 gccacccgct ggatcgcctg cgcgaagtcc ggcgagggca gcatgtgacg caggatcagc
     4381 aggccatccg gctgcgcgcc ccacgcgatc cagttcacgc cgcactcggg cctggcgttg
     4441 gccggacgct cgcccggctc gctcatgacg agcgtgaacc gcccgtccgc gcgcgtgcgc
     4501 acctgctcgt cgaacacgca gtcgatcacg cgctggctgg cgaactcgtt ctcgcacagc
     4561 gaccagtacc gcacctggcc gccctccatg acgcggcggc cgtcgagcgt ccgcggagtg
     4621 gtcggcgcaa ggccctcgag caccgcgacc gggccgatcc cccggttggc cgtggccagc
     4681 gtgtacgcgt tgtcgatgtt cgagaggaag ccgccgagcc tctcgcgcgg cagcacggca
     4741 cgccccgacc ccgccggcgt cgccccggcg gggtagctga acgcctggaa gtagttgaag
     4801 aacgcctccc agcggatcgg gtcctccgac gtcgggttgg acgagtccgg tccgtcggtt
     4861 cccgagtgga acgcctcgac ggcctggttc gcgccggtgt cggcgcgcgc ctggcagacc
     4921 agcggctggt cgagcacctg accgtccggg agccgcatcg agacctccgc cagccccacg
     4981 ccgccggccg ggtcgcgccc cgcgtccggc acgtagacgc ggtacatgat caccccgaca
     5041 ggcgtcccct gccccgccgc gttcaggtag atcgtgttcg gctcgcgctt ctccggccgc
     5101 gcgcccccga ccacccgcac cgtgtactcg cgccgctcca ggtctcgctg cgcgccgggc
     5161 gggaacgggt tcgccgcccc ggggtcgggc gcgatgtcta tgtctgcgag cccgtcggtc
     5221 ggctgggccg cggcgtcgta gacgttgaac gacatgtagc gcgcgtgcgg atagcggccc
     5281 ctgaggatca gctcggcgcc gggcggcagc gggaacgacg acacccagta gcgcgcgccg
     5341 aggtcggggt aggcgatgtt ggtggagcgg gtgccgccct gccagaagca atcgcgctcg
     5401 tcctgagccg ccgccggccc ggcggcgagc agcgcgagcg cgatcgcaag ccaaccgccc
     5461 ctccgcgccg cccccatgcg gcgattatgc cgggcggccg gtgccgaccg ggctactcgt
     5521 cggccagcgc cagcagaccg gtcacggtgg tccagttgcg cgccgtgccg gtcggcatgc
     5581 gcttgctggt gcgcagccgg tccaccttcg agcgctggat cccgtccggg tgccacgtgt
     5641 agagctcgcg cccgatcagc gcgatgcgct cgggcgcgac gtccatctcg gccagctcct
     5701 gcgcgagctc gtcgctcggc tcctccgaga ggaaggtcac ctggtagagc tttgggttgt
     5761 ccacgtccga cccgaacggg tcgcgctcga tcacctgcgc gagctcgtcg cgcgtgcgga
     5821 ccaccaccgg tgtgtccacc ccgaggccct cggcgatctg gcgctggaga tcgcgctcca
     5881 ggcgcgccgg cgagagccgg ctcgtgagca cgatgttgcc gctctggacg agcgtcttca
     5941 cgtcgccgta gccgtgcccg gcgagcagct cgcgcagctc gggcatgctc acccggttgc
     6001 ggctgccgag gttgattgcg cgcagtagcg cgatgtggcg cggcacgtcg cgatggatag
     6061 caagccgcgc cggcgcacgg cgcccgcctc aggcgtagcc ggtcgcgctc aggcaggcat
     6121 cgatgccgcc ggtgtcggcg tcgggcggcg cggccaccac cgcc
//
LOCUS       DASVPL010000292         4268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_74609, whole genome shotgun sequence.
ACCESSION   DASVPL010000292 DASVPL010000000
VERSION     DASVPL010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74609"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VF517_16350"
     CDS             <1..617
                     /locus_tag="VF517_16350"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX8054559.1"
                     /translation="TREMEALDALLWQLGLATKVPGWLITDRGSRIKFNRSFYRSYEH
                     LPAAELRDQTKDSLAEFILPRIQNEAVRRIRAHRRRGDRVVLMTGGLDFLMEPLRHLG
                     DDLVAARLIEERGAFTGELAEPPLTADGRASFAAQMAADHGSELADCYAYGDSLSDLP
                     LLDTVGHPYAVNADSGLTREARRRRWPLLEWKTEQRARVLEAAR"
     gene            614..1768
                     /locus_tag="VF517_16355"
     CDS             614..1768
                     /locus_tag="VF517_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8054560.1"
                     /translation="MIDALEYRPSLPKLVASRALRRPVHGALRLTQQTIPRRPAPEWV
                     NVRPRLSGICGSDQALLAGHASPYLGALTSAPFVLGHEVVGDVLSGPNAGARVVVEPA
                     LGCEVRGITPPCVECLEGRYALCRNTVSGNIAAGLQTGVCRDTGGGWSEGLVAHERQL
                     HAVPDALPDEDAVLVEPLACAIHAVRETDPAPGSHIAVIGAGTIGLLVLAALRERASG
                     TEVTCVAKYESQKLQTLRFGADHVTTPDRATLDAARITAARRLVGRFGHELMLGGFDA
                     VLDCVGSASSLRTALSIARPRGRVMLVGMPGRIGIDLSLAWQRELEVRGVYAYRDDFA
                     EALRMAGEIRPGRLIADGWRLRDHGRALQEARRGARNGHPKTVFDLREAA"
     gene            1765..3408
                     /locus_tag="VF517_16360"
     CDS             1765..3408
                     /locus_tag="VF517_16360"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX8054561.1"
                     /translation="MTINATPPPVVERGWRSAAPARARRAGTILEIHEKSPPTLFFDG
                     PSMRLERLPVGTRVIYPQPSLRGLADVDAAISAALGNPSDMEPLEALLKPGMRLTISF
                     DDVSLPLPQMRPPDIRGRIIEEIVARAYRAGVDDIHLIGATAFHRRMTEAELKHLLGP
                     NVFAEFYPDRLYSHDAEDPDNIAFLGETEKGEKVELNRRAAESDLLIHVSVKLTPMQG
                     GRKSVPVGLGTYQSLRAHHTAHALKQSRSFLDPENSELHHSSGRQGDVVASAVRLMQI
                     EVTLNNDSYPRSLSFLGKPEPMWTRREQLTFASMARAMRRLPANRRSAAIQGMRAPYQ
                     VTGIFAGEVNAVNAAGAESCAAQLEVPVEGQTDILTTGVHDIGPYNVNSTMNPILVMC
                     MALGYYFNLYHGKPLVREGGVLIFTHPVRREFHPVHHPSYIDFYEEVLPQAKDPAEVE
                     RFEQSYAEDEWYRHLYRTSYSFHGAHPFYMWYWGAHALDHVGAVIFVGGDPEVCRHMG
                     FRSASTMQDAIEMAKDVVGTSSPSMTQLHFPPAMYCKVT"
     gene            3405..4211
                     /locus_tag="VF517_16365"
     CDS             3405..4211
                     /locus_tag="VF517_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8054562.1"
                     /translation="MRVAGGGRGLERLAGTQRRHARPKPRWRASAEGADAWSREADDE
                     AWLHGRVPSLAREALQQTLLFPLVRTIARPAIMGADDLLHVPQPAVLAPNHSSDIDTP
                     LVLSALPRSWRAKTVVGAAADRFYRTRKIALATELWINTFPFDRSGELRGLAAASIFL
                     RDGYNVLLYPQGTRSRGQLNGFRTGVGRLCVATQVPLIPIHVGGTSLLMPKGRGLERR
                     GHTTVQFGCPLFPGEDETPAEFTRRASEAIAELSANSVRLRRVQSALQNV"
BASE COUNT          637 a         1518 c         1495 g          618 t
ORIGIN      
        1 ggacgcgcga gatggaagcg ctggacgcgc ttctctggca gctcggcctc gcgacgaagg
       61 tgcccggctg gctgatcacc gaccgtgggt cgcgaatcaa gttcaaccgg tcgttctacc
      121 gcagctatga gcacctcccc gccgccgagc tgcgcgatca gaccaaggac tctctcgcgg
      181 agttcatcct gccgcggatc cagaacgagg cggtcaggcg aatccgtgcc caccggcggc
      241 ggggcgaccg ggtcgtgctg atgacggggg gcctcgactt cctcatggag ccgctccgcc
      301 acctcggcga cgacctggtg gcggcgcggt tgatcgagga acgcggcgcg ttcaccggcg
      361 agctcgcgga accaccgctg acggcggacg gccgcgcgtc gttcgccgcc cagatggcgg
      421 ccgaccacgg atcggagctc gccgactgct acgcctacgg cgacagcctg agcgacctgc
      481 cgctcctcga caccgtgggg catccgtacg cggtcaacgc ggactctggg ctgacgcgcg
      541 aggcgcgccg gcgccgctgg ccgctgctgg agtggaagac cgagcaacgc gcgagggtcc
      601 tggaggccgc gcggtgatcg acgcgctcga gtaccggccg tcgctcccca agctcgtggc
      661 ctcgcgagcg ctgcgccggc cggtccacgg agcgctgagg ctgacccagc agacgatccc
      721 gcgccgcccc gcgccggagt gggtcaacgt gcggcccagg ctcagcggta tctgtggctc
      781 ggaccaggcg ctgctggccg gccacgcgtc cccgtacctc ggggcgctga cgtcggcgcc
      841 gttcgtgctc ggccacgagg tggtcgggga cgtcctcagc ggcccgaatg cgggcgctcg
      901 cgtcgtggtc gagccggcgc tcggctgcga ggtgcggggc atcacgcccc cgtgtgtcga
      961 gtgcctcgag gggcgctacg cgctgtgccg gaacaccgtg tcgggcaaca tcgcggcagg
     1021 cctgcagacg ggtgtctgcc gggacaccgg cggcggctgg agcgagggcc tggtcgcgca
     1081 cgagcgacag ctccacgctg tgcccgacgc ccttccggac gaggatgccg tgctggtcga
     1141 gccgctcgcg tgcgcgatcc atgccgttcg cgagacggac cccgcaccgg gctcgcacat
     1201 cgcggtcatc ggcgcgggga cgatcgggct cctggtgctc gccgcgctgc gggagcgcgc
     1261 gtccgggacc gaggtcactt gcgtggccaa gtacgagtcg cagaagctcc agacgctccg
     1321 gttcggcgcc gaccacgtga cgacaccgga ccgcgccacg ctggatgccg cccggatcac
     1381 cgcagcacgc cggctcgtcg gccgcttcgg gcacgagctg atgctcggcg gcttcgatgc
     1441 ggtcctcgac tgcgtgggca gcgccagctc gctcaggacc gcgctctcga tcgcgcggcc
     1501 gcgcggtcgg gtcatgctcg tcgggatgcc ggggcgcatc gggatcgacc tctccctggc
     1561 gtggcagcgc gagctcgagg tgcgcggcgt ctacgcgtac cgcgacgact tcgccgaagc
     1621 cctcaggatg gcgggcgaga tccggccggg gcggctgatc gccgacggct ggcggctgcg
     1681 cgaccacggg cgcgcgctcc aggaggcgcg gcgcggcgcc cgcaacggac atccgaagac
     1741 cgtcttcgac ctgcgagagg ccgcatgacc atcaatgcca ccccgccccc tgtggtcgaa
     1801 cgcggctggc gcagcgcggc gcccgcacgc gcgagacgcg ccggcacgat cctcgagatc
     1861 cacgagaagt ccccgccgac gctgttcttc gacggcccgt cgatgcggct cgagcgcctc
     1921 cccgtcggga cgcgcgtgat ctacccgcag ccgtcgctca ggggacttgc ggatgtcgac
     1981 gcggccatat ccgcggcgct cggcaacccg agcgacatgg aaccgctcga ggcgctgctg
     2041 aagcccggga tgcggctgac gatctcgttc gacgacgtgt cgctgccgct cccgcagatg
     2101 cgcccccccg acatccgcgg gcggatcatc gaggagatcg tcgcgcgtgc gtaccgtgcc
     2161 ggtgtcgacg acatccacct gatcggtgcc accgcgttcc accgtcgcat gaccgaggcc
     2221 gagctgaagc atctcctggg gccgaacgtc ttcgccgagt tctatccgga ccgcctctac
     2281 agccacgacg ccgaggatcc cgacaacatc gccttcctcg gcgagacgga gaagggggag
     2341 aaggtcgagc tgaacaggcg ggcggcggag tccgacctgt tgatccacgt ctcggtgaag
     2401 ctgactccga tgcagggcgg tcgtaagtcc gtgccggtcg ggctcggtac ctaccagtcg
     2461 ctgcgcgcgc accacaccgc tcacgcactg aagcagtcgc gctcgttcct cgatcccgaa
     2521 aacagcgagt tgcaccattc gtcgggccgg cagggggatg tcgtcgcgtc cgccgtgcgg
     2581 ctgatgcaga tcgaggtgac gctcaacaac gacagctatc cccgctccct ctccttcctg
     2641 ggcaagccgg agccgatgtg gacgcgccgc gagcaactca cgttcgcgag catggcgcgc
     2701 gcgatgcggc gtctgccggc gaacaggcgc agcgctgcga tacagggcat gcgcgcgccc
     2761 taccaggtca caggcatctt cgccggagag gtcaacgcgg tgaacgcggc gggcgcggag
     2821 agctgcgcag cccaactcga ggttcccgtc gagggccaga ccgacatcct cacgaccggt
     2881 gtccacgaca tcgggcccta caacgtcaac tcgaccatga acccgatcct cgtgatgtgc
     2941 atggcgctcg ggtactactt caacctctac cacggcaagc cgctcgtgcg ggagggtgga
     3001 gtcctgatct tcacccaccc ggtccggcgc gagttccacc ccgtccacca cccctcgtac
     3061 atcgacttct acgaggaggt gctgccgcag gccaaggacc cggcggaggt ggagcgcttc
     3121 gagcagagct acgcggagga cgagtggtac aggcatctgt accggacgtc gtactcgttc
     3181 cacggcgccc acccgttcta catgtggtac tggggagccc acgcgctcga ccacgtgggg
     3241 gccgtcatct tcgtcggcgg cgatccggag gtgtgcaggc acatggggtt ccgcagcgca
     3301 tcgacgatgc aggacgcgat cgagatggcg aaggacgtgg tcgggaccag cagcccctcg
     3361 atgacccagc tgcacttccc gccggccatg tactgcaagg tgacgtgagg gtcgcgggcg
     3421 gcgggcgcgg gctcgagcgg cttgcgggca cgcagcggcg tcacgcgcgg cccaagccac
     3481 gctggcgagc gtcggcggag ggcgccgacg cgtggagtcg cgaggcggac gacgaagcct
     3541 ggctgcacgg ccgggttccg tcgctcgcgc gggaggcgct ccagcagacg ctcctgtttc
     3601 cgctcgtacg cacgatcgcg cgccccgcga tcatgggtgc cgacgacctg ctccacgtgc
     3661 cacagcccgc ggtgctcgcg ccgaatcact cgagcgacat cgacacaccg ctcgtgctgt
     3721 cggcacttcc gcgctcgtgg cgggcgaaga ccgtcgtagg cgctgccgcc gatcgcttct
     3781 accgcacccg caagatcgcg ctcgccacgg agctgtggat caacaccttc ccgttcgacc
     3841 gcagcggcga gctgcgcggg ctggcagccg cctcgatctt tctgcgcgac ggctacaacg
     3901 tgctgctcta cccgcagggg acccggtcgc gcggccagct caacggcttc cggaccggtg
     3961 ttggccgcct ctgcgtcgcc acacaggtcc cgttgatccc gatccacgtg ggcggtacgt
     4021 cgctgctgat gccgaagggc cgcggcctcg agcggcgcgg acacacgacg gtccagttcg
     4081 gctgtccgct cttcccgggc gaggacgaga cgccggccga gttcacgcgg cgcgcgagcg
     4141 aggcgatcgc cgagctgtcc gccaactcgg ttcggctccg ccgcgtgcag agcgctctgc
     4201 agaacgtttg aggagacccg cgcgagccgc taccgcttcg ccatcaaccg tttcgcgagc
     4261 ggcagcgt
//
LOCUS       DASVPL010000293        30763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_230895, whole genome shotgun sequence.
ACCESSION   DASVPL010000293 DASVPL010000000
VERSION     DASVPL010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 30763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30763
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230895"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1723
                     /locus_tag="VF517_16370"
     CDS             173..1723
                     /locus_tag="VF517_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054563.1"
                     /translation="MAITIFAAIRLRDTYVGDAAVYLPYARNAGDGHPFQFNLGEFSS
                     GSTSPLWSLILGVPFLAGFELMGAKAMAALFAAIGFLTTLLAARHFSRSWAAAGVAAL
                     FALGTMTFPAVSLYESGLVVTLSALALLAGDHVLGAWRATDAPRAPAWRTLAPLVAVW
                     AALPLARPDAVILVGAHALALLAFAPVERRRALRTLAPGLAVAALPALAYFGYSVAEL
                     DTFSTSSQARTFALREGARDWLGPLFLEDGAVRELFASPWIFGLLPALAGLWLLWRRA
                     ETRWAAAYGALAVVGYVLLLTFVAPGIYDTPRYLLPLVPVVACGVAVLLARPAQPLLA
                     WAALGLAALAIGGSAADELRDNVRLARSIGIDRDEVFERDVVARIDALAEPGDAVLAY
                     EVQLRYFLRQDVKVFSQDGITDPRVAAYREDKDMTGYLMRYRPEFWIADRNAQTRQYT
                     DGTILERALVAYRHSPRPAVRVFDGIRFELVARRNRPLALGFGGWEFLFRLSYPPPRG
                     GRSHLPSQ"
     gene            complement(1746..3326)
                     /locus_tag="VF517_16375"
     CDS             complement(1746..3326)
                     /locus_tag="VF517_16375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8054564.1"
                     /translation="MRVLHVGDDNAVLRPCGLTFYAEALMDAQRAAGHEVAYLFSGRY
                     FPRLERPRLKRSRRRGVRTFELIGSPINTHTERGTRHPERDIDEPVGDAAFAAALREA
                     RPDVVHVQELLRLPSSILERAREAGVPVVMTLHDYKPLCASVRLIDADGARCMRREVG
                     EDCARNCAGAPEGNNHLIEATLAHYRRRAKRLVPGGEDLDFGRVAPLVGTVNRLLVRA
                     QPHGLPEVDVRSHGVEPAAGPVPDEPTLPPLDPEPFQRRRDVNVGRLSMCDRLVAPSP
                     RVAEIYAELGVDPGRIVVQRLTLPHLAELAPRRGAEPGAPLTFVTLGAAASETKGSRA
                     ILDAVRALEAAGRGGSYRLVVHGGVERSIHDELEAIGSVELAGPYRPEDLDGLLDAAD
                     VGIVPSVWEEVHGFVGIEMLAKGLPLIGSALGGIPEYVREGETGWLNRSASGAELAEL
                     MARAIDDPAEVERLRRSVRERRAELVRPMADHAAEVEALYRELTGGVGGVGGEGGEGG
                     EGGEGGGGGEGGARTLHH"
     gene            3351..4070
                     /locus_tag="VF517_16380"
     CDS             3351..4070
                     /locus_tag="VF517_16380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054565.1"
                     /translation="MSGSHMERFWDARAREDALWYVDSRLEFGDGDEEAFWGGGEEAL
                     DRLFEVLGARPDPGAVIVDIGCGVGRLTRPLARDAQRVIGIDVSSEMLDRARELNAQL
                     DNVEWLHGDGESLRPIEDASADVCVSHVVFRHIPDPAITLGYVREIGRVLGLGGFAAI
                     EFSNDPEPHKRRAVRDAKTKLAAAVGRKPRGIADAEWLGSYIDLADLRRAAAGAGMEV
                     ERVVGEGTEYCAALLRKGAPA"
     gene            4067..4777
                     /locus_tag="VF517_16385"
     CDS             4067..4777
                     /locus_tag="VF517_16385"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054566.1"
                     /translation="MSTVDNYYDQFWSGEKERHYQPESVLEELILEGVTSTTAVLDVG
                     CGSGNSYAGELNRRAASYVGVDVSANAVEAARGLGLDARTIDAAASLPFEDESFDLVV
                     CIEVMEHLFAPHEAAAEIRRVLKPGGRLVCSTPNVAYWRLRANMVFGLWNPLGDELAL
                     ERPWRDPHIRFFTPKTLARMLRMSGFRDVRTGAHGGRFLDHASSRPTDFGQGRAYAAI
                     EPLLPSLLGLTIHAVAVK"
     gene            4807..5355
                     /locus_tag="VF517_16390"
     CDS             4807..5355
                     /locus_tag="VF517_16390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8054567.1"
                     /translation="MRWFATATRPYWRRRFAEFGSESLLYRPAWVHRPELMAIGSRVW
                     ISQDAWFEVGGEPGGPPRIRLGEGVVLRHHVTLSAFESVTIEPEVLIAAYTSIYDSDH
                     TIGRDRNAIWYPHRVEPVRIGFGSWLGERVQVLKGADVGRHCIIGANSVVRGTIPDYS
                     VAVGAPARVVGDTREMVERSEP"
     gene            5352..6689
                     /locus_tag="VF517_16395"
     CDS             5352..6689
                     /locus_tag="VF517_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054568.1"
                     /translation="MIRGLLRTPPRPLSGPVRPPLLPAAAYVVAMTALQMLRRPHDPP
                     AWDSLFVEDGEIFLQGAISQGLVSSFTTAYQGYLHVVPRTIAEVAGQLPLERAPLVMA
                     LLATGLVSLLSVYVYCASAAWIGSRVLRGALAIAFVLLPAGLLDMNGVTSYVSWYMLY
                     AAFWALASPWLTRGWIAVSSVLVLLTALSNPLLGFFLPVAIVMALRSGERRAWIVPGM
                     ASAGLFVQLLAREPPETFGETMPGEIPRIFADRVPTSLLTGERFLEDLLGGTSGSFFA
                     WASLAVVAAAVGFGLVRLRGRRAWLLGGCALAAVVIFTTAALIRGTEQFLPDDPWLGF
                     ASRYVYPAVLFLLTGLLAAVDRAEAAPRLGTPREWAAAVALLAVIAVNLPVDHHAVRG
                     PRWGPQLEAARERCDRPARERPREVVIPTAPYTPPGTERGPFTWELRVSCDAL"
     gene            complement(6670..7818)
                     /locus_tag="VF517_16400"
     CDS             complement(6670..7818)
                     /locus_tag="VF517_16400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8054569.1"
                     /translation="MPNTRPLRVLLICSGLDHAHRGFESFARECFDALRDDPALDMKL
                     VKGSGAAGDRERSVPTLTRDSAPVRALARLSGRQAFRFEHVAFAFSVMPVLLRERPDV
                     VFLSEWHTGKVLAPLRDAAGRSFALVLSNGTMALEGFGHLDRVQELSPAALEEILARG
                     ADPERHVMLPLGFWIDPQVEPVTDDDRRAARERLGLPLDRRILLSSAALNRHHKRLDY
                     LIEEVASLPEADRPYVLMAGQVEEETEGLRALARERLGPDGHDMRTVPQRQVPELLRS
                     ADEFVLASLGEGLPRALIEALAAGLPCLTHDYGVTRYALGEHGHFADFSRPGGLAGLI
                     AGAGFDGSPDAQRARHRYAYEKYSWEGLRPRYVELFRDAARAARARRS"
     gene            7863..9344
                     /locus_tag="VF517_16405"
     CDS             7863..9344
                     /locus_tag="VF517_16405"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8054570.1"
                     /translation="MALAQEDAPTNLRARTARGTLINAIFMVAVSMLALVQGVALARL
                     LSPETYGLWGMLMAAFITLLTLGSVGIDDKYIQQDDEDQQRAFEIAFTIQILLAPVFV
                     VLLLIGIPLLALVYGETRIIGPGLAIAFVIPALALQMPLWAHYRRMDFLRQRLLQSID
                     PVMTFIATITLVLAGLELWGLVLGAMIGSFAASAAMVATSPYKLRWRFDRDALREYRK
                     FSTPLFVAAGSTVLLVQVPVIVGSNVLGVTAVAAMALAGNISQFTNKVEDLVTGSMYP
                     AICRVKDRTDLLFEAFWKSNRLALLWAAPLGAAAPLFVGDFVHHVIGEKWRFAAPLIG
                     IYGFLAVFSQIAFNWTAFFRAVGDTKPIATASVVNTAAVIAFGVPLLVLEGVTAFGVG
                     VAVATVIGVGLRLWYVRRLFPGMPFVSHIARGILPTVPGVVAVLALRLADPGGRTVGR
                     VALEAGLYTVLIVLTTWFSERSLLRESVSLLRGGARLREAPVA"
     gene            9341..10261
                     /locus_tag="VF517_16410"
     CDS             9341..10261
                     /locus_tag="VF517_16410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX8054571.1"
                     /translation="MSAIGIVIPTLGRHALLRRVLDRLDRQTAPQGSFEVVVVADAKE
                     ERLDEIDRALEGRSFPARRLRGPRPGASAARNEGWRALDSPLVLFVDDDILPEPELVA
                     QHLGWHDRHPQEEIGVLGHVRWADELDVTPFMRWLEHGIQFDYPNIDGEDAGWARFYT
                     ANASVKRTVIERVGGFEEERLPFGYEDLDLALRMHEQTGFHLLYNRAAVAEHVHPMDL
                     EFWKRRVARIAISEREFVRMHPDFRPYFYELFSKAASDPRANGRGERLARFVPRSLPV
                     LGERVWSSADAVYRQALAGPFLEAWEAAGG"
     gene            complement(10204..11445)
                     /locus_tag="VF517_16415"
     CDS             complement(10204..11445)
                     /locus_tag="VF517_16415"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8054572.1"
                     /translation="MRLGAPDGAADAPPEAREHATQPSDAAPRLDRFLAGDGLRLLAA
                     VGVLVFHALVVASTTHPDATPDDSIDARIANLGRIGLWVFFVLSGYLLARPFVAAFVE
                     RRELPSVRNYMRNRLLRIVPALWLAAILTLIVFGRQGAGLGQIAAVFLFVQEADSSAF
                     SRHMLHAWSVDVELAFYLLLPLAAAALFWIARRTRVPRAGLVWLLLAGVWLASLAFQR
                     ALESNTLLSDGWQRSPPAVFYAFVPGLVLALVEVQFGARLRGLRGARVLVTALLAGGL
                     AIATVHTFQLPADSAWKPSITAALAAGAIVGSALMLQWTTGGAWRALTNRPIQWLGER
                     SYGIYLFHLGAMLWIDDRIDVSHGARRAILIAGAFALATALAALSYRFVEQPFLRLRH
                     GWRQPPAASHASRKGPASAWR"
     gene            complement(11442..12104)
                     /locus_tag="VF517_16420"
     CDS             complement(11442..12104)
                     /locus_tag="VF517_16420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8054573.1"
                     /translation="MKELLKTVLPPRVRDRLRPLPQRLGYYPGPGPRLMSWLRMQWVI
                     LRHPQANISFGHGCYLGPGFSLHIPAGGTFEAGDGVEFRRGFRAEVDGDGRITIGSHS
                     HMTYYVLMQCSTLIEIGEHVQFGQSTIVVDGQHRFRDLTKPMLEQGYDFAHVKIEDHA
                     TITTKCSIMADIGTRSFIGANAVVSKPIPPYCVAVGAPAKPIDYFGPPGQEPEELRST
                     TA"
     gene            complement(12101..13315)
                     /locus_tag="VF517_16425"
     CDS             complement(12101..13315)
                     /locus_tag="VF517_16425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8054574.1"
                     /translation="MRVLTVGNLYPPHHFGGYEQVWRSAVRHLRANGHTARVLATDWR
                     LDGVADGDEPDVHRELRWYWAGHDFPRIPLAQRPPIERHNAHVLARHLDELEPDVVSF
                     WSMGGMSHSLIERVRRRGLPMVAFVHDQWLDYGRSFDQWMRLFHAPRRRLAARAVDRL
                     TGIPTRVDYAGAGRYVFVSDFVRRRALKLPEPVRDTAVAHSGIDPALVGPLPPEHDWS
                     WRLLYVGRIHHDKGIHESVDALAHLPGEATLTFAGKWDERDEDALVERARGLGVESRV
                     TMLGQRSPAEVAELMRAHDALLFPVLWEEPWGLVPIEAMAQGLPVVATGRGGGGEYMR
                     DGENCLIVPARDPEAIARAVERLAADPELRRTLRAGGERTAPLHTETVFNEQVEGHLL
                     EVAGHAWEPVAA"
     gene            13349..14296
                     /locus_tag="VF517_16430"
     CDS             13349..14296
                     /locus_tag="VF517_16430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8054575.1"
                     /translation="MPPRVVIAVVSWNTRELLDRCLLSMKPHADAGAASVWVVDNAST
                     DGSPEMVRECHPWVELEARDDNLGYGRALNLVAERTESEWFAISNADVHLREGALERL
                     LAAGDADPGAGAVAPRLILPDGSTQHSVWAFPTLAATAVQNLGPRLSPGDLAERMAIR
                     GSWNPDRAGRVPWAIGAFMLIRRRAWDDVGGFDPGQWMSAEDLELGWKLRETGWATYY
                     EPAAVVDHEESAATGEVWGEDLPLHWQRCAYAWMVRRRGRARTAAVGTMNFAGSGARW
                     ARGMARARGRPDPGLRALGRWTLVHRYAFAPRRVLGRYR"
     gene            14296..15057
                     /locus_tag="VF517_16435"
     CDS             14296..15057
                     /locus_tag="VF517_16435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054576.1"
                     /translation="MRGAERLRRVARRAYYSRRGVRELAALGRLPLLRREGPDLAHLA
                     FYEEIAEGPVQRDEALFLHALIRVVRPQTVVELGFLRGHSTFNFLRALDPGARVYSFD
                     IDPACEDFARERFSHDPRLTYRTKSQDELTPGDVDGRSAEFVFIDAAHELSLNQATFE
                     RLLPLLAPDAIVAIHDTGTVPRRFVAPDHFTQGIPQRWVGDAVEHQPDERAFVNWILA
                     AHPEFAQLHLHSRHALRWGLTLLQRSAPLPRPAGI"
     gene            complement(14983..16119)
                     /locus_tag="VF517_16440"
     CDS             complement(14983..16119)
                     /locus_tag="VF517_16440"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8054577.1"
                     /translation="MLSARAVKPGTSRGNNFDALRLVAATTVLVLHCWPVVGQSTVPR
                     VHGLGLNAAAVMVFFVISGFLLAQSWTLEPRLGRFLAKRALRIYPALILVLVLSTFVL
                     GPVVTTLPVREYLSHPETWGYLYNNLVFETRYSLPGVFPDNPIPLAVNGPLWTLSPEV
                     RSYAMLALVGLVGFLRSRVLTAGLLAVLLVGPELAPERAGDLMVAPLYVQCFAIGSFL
                     YVFRQIVPWHWAIAAGLVAAWLVVGGDAAPAAGGSPTTLLAALAIPYTTIFLAYNTPK
                     RIQLLTDRGDLSYGIYIWGSPVQETVQHLWGRGLSPLGLLAMALPITVLCAAGSWFLV
                     ERPALRFKARRVSKRPMAEDLDPGRSRQRRAALKQGETPAESVP"
     gene            complement(16135..17271)
                     /locus_tag="VF517_16445"
     CDS             complement(16135..17271)
                     /locus_tag="VF517_16445"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEX8054578.1"
                     /translation="MPRVMIVGGGLTACTVAHRLAREGIESVILEREDVHGGLVRSDH
                     MNGVLYEPHGSHIFHTEDKEVWDLANEMTPFHDYRHRVDIVVEGKILNWPILLSDIDA
                     QSKSEQIKKELDERRDVDAAGRAQSANFEEWCLELMGPTLYERYIRPYTQKQWGRPPR
                     ELSAGWAPRRVSVRWDNDPYLFPDPYQGWPSGPNGYTDLLDGLLASDGIEVQYGHDVT
                     LENVEEHAARVKPDAVVLTCPLDEFSGFKLGRLDWRGILVRSVYVPHMEYAQGAMVVN
                     YPGLEYPFIRIHETKHASRQKVDGTVLGFEFTGAPTRYYPIELPENRELNNRYQADLT
                     ERLSALNVFYAGRLSNYVYIDMDDCMRQALDTAARMLEELPAPA"
     gene            complement(17291..18223)
                     /locus_tag="VF517_16450"
     CDS             complement(17291..18223)
                     /locus_tag="VF517_16450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8054579.1"
                     /translation="MTPAVSVVVPTHNRPDGLATVLAALGRQTLPHDRFEVIVADDGS
                     DPPARPAAEDVALRVVRHERPRGPAAARNSGWRAASAPLVAFVDDDCEPTDGWLAALV
                     EAATDAGEAVVIQGPVQPPPNQLAELTPLSHTIVVDGPDRLFATCNIAYSRPLLERLG
                     GFDETFRRSAEDVDMGARARAAGARALWAAGALVHHEVRRLGLRDLIRHTTKWTDSVR
                     AVGAHPELRELLVARVFWKPTHPRLLLALAGLATRRPLLAALAALPYLDHYRRLYAHD
                     PSAGARALPTHLAVDLTEIGTAVAGSIEHRTLML"
     gene            complement(18220..18957)
                     /locus_tag="VF517_16455"
     CDS             complement(18220..18957)
                     /locus_tag="VF517_16455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054580.1"
                     /translation="MDTTFYSEYYEIEDRHWWFVGRREVLMRVLADHLEPGRDRRILD
                     VGCGTGTMLAHLGRFGTAQGVDASEEAVAFCHQRGLDDVGLLDDGPLPFGDDTFDLVT
                     AFDVIEHVDDDLGLLRDFRRVVAPGGHVMVSVPAYRFLWGPQDEISHHKRRYVRRELT
                     ARFEEAGLTVLRSTYFNTLLFPPIAAIRVLRPAKPAEELESDFTLTKPGRVNSLLGRV
                     FASEARLVGRTDLPFGVSILALARNDG"
     gene            complement(18960..20327)
                     /locus_tag="VF517_16460"
     CDS             complement(18960..20327)
                     /locus_tag="VF517_16460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8054581.1"
                     /translation="MALAAALYLWQLGSSSLFVDEVPTHRFIRGDLGDLFDGVARGET
                     SPPTYWLVLNLWNELFGSSEDWIPRLLSVVFAVALVGVVYRLGVLLGGRWAGIAAGLL
                     TALSPLVLGYAQQARQYALLMLVATFAVLALVEAEQRGSRRWLIAAGVAACGAVSLHY
                     VALLVVGPLCAWVLLRSSFGLRARAVFCAVPALVFFAWVPLMLHQYDLHPNGGLGEFG
                     ELSRGHAVRVTGSPFDIRTADEATIVQILGALVVMAAAGVLAWRLVRERRRDHELVLL
                     IAVAAPLGLLAAGIAGKDMVATRYAAVAVPTMIVMIGVAIAAAPRVPRAALVAAALVA
                     AVLGTVRTHQEDGLFPDARGAMETIASDWREGDAVVDQSGSFGVDLPLMFYATRELPP
                     GAPVVAASDPRAERLTADGRRVWIVTSDPTPALARANHLPAGRRLAMARHFPDGPGIT
                     LIRAE"
     gene            complement(20383..21165)
                     /locus_tag="VF517_16465"
     CDS             complement(20383..21165)
                     /locus_tag="VF517_16465"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8054582.1"
                     /translation="MVRGLSIVLPAYNEEQNVRTAVERALGVLEQVAEDYEVIVVDDG
                     SSDGTSDVVRSLVDEHGDRVRLIVHETNIGYGAAIRTGFDCSRYEFVFYTDSDNQFDV
                     AELEYFLPFVEEHDVVIGFRVYRYDTVLRSIMSWVYNRLVGVLFRTRVRDVDCAFKLM
                     RRQVLDHLSLECDNFFIDTELVARARKWNYRIAQKGVRHYPRLAGATTVRPSDVPNTL
                     REVLRMWQRIYFPTRAQLEQYAAHEQHRRARTSEYVPAAVGS"
     gene            21221..22267
                     /locus_tag="VF517_16470"
     CDS             21221..22267
                     /locus_tag="VF517_16470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8054583.1"
                     /translation="MPLPTVSICILVYNRREELRETLQRMLEQSDYPRELLEPIVVDN
                     ASSDGSGDMVREEFPDVKLIARDENIGASAWNDSFRIATGDYVLVLDDDCYMPPDGLT
                     RAVAAAQEHEADLVSFAVESSVMPGYYFSRAYKTGLFSFWGCAWLIKRSVVQELGGYD
                     PEIFIWANELEFTMRFFDHGYKHLHDPTIVAQHMKHPGDDTLDIEERGYKINANHWGY
                     IAGKLFQPRDAAEALLALALRCVREAVRLDAVAIKGVPETFKGFRHGLRHRRPVRPEV
                     SRFYRHNFETFASPWWMARPVRELVLELPRELVRERTTRNPPKHRGRRDEYYEKRAHL
                     YPLERPEALQLRQS"
     gene            complement(22253..23158)
                     /locus_tag="VF517_16475"
     CDS             complement(22253..23158)
                     /locus_tag="VF517_16475"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8054584.1"
                     /translation="MSTLLVAATGGHLSQLVRLEPRLGLREEPTWVTFDTGQSRELLA
                     GREVVHARYTRPRDYAAVARNVPLALRLLRSGRYERVVSTGSGIALSFLPQAALLRRE
                     SHYVESAARAAGPSVTGRALARVPGVRLYTQYPAWAAGRWRYAGSVFDAYEHVPAAAE
                     DRPLRVALSVGTLFPFPRLVERLRAVLPEDAEVLLWQSGEDTTVPPAELRAAFRAADA
                     VVVHSGVGAALEALDAGRRPLLVPRLKEHGEHVDDHQLQVAGELARRGLAVVCRPEEL
                     TADHLHEAASGRVTAVADPPPLRLT"
     gene            complement(23155..23913)
                     /locus_tag="VF517_16480"
     CDS             complement(23155..23913)
                     /locus_tag="VF517_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054585.1"
                     /translation="MALLTVLLTHLGRAQVEAQLAQMRALAPDGRFVAVHGGRRSDFD
                     GLDPADALFVEDPSLRGPHFDKSLNDTLRAIHDRWVRDDPDVDLVYLVEYDHVILRGD
                     FEAALRDVVARTGAGLVAKNATPRNDTNWSHFLKVRGDERLDRYIESITTRDDPLVRW
                     GCLGTGLLLTRDALSAFRALDEPPPHYVEMFVPTAVHHLGFHVVDMDAVSDLYADVRW
                     LPEFGVEEALAAKRAGRHFVHPFKRLDELAALSA"
     gene            complement(23918..24187)
                     /locus_tag="VF517_16485"
     CDS             complement(23918..24187)
                     /locus_tag="VF517_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054586.1"
                     /translation="MRALRARVARSRIGRGAEAALLRALGRPLRCAECDRVLFRGIPF
                     VWRGRLRIVGARESHVRIAFTAKDTLELRHVELDQCPSPDRPWVR"
     gene            complement(24184..25128)
                     /locus_tag="VF517_16490"
     CDS             complement(24184..25128)
                     /locus_tag="VF517_16490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8054587.1"
                     /translation="MEEHPLPVSVVIPAYRRPDLVERAIRSVQAQTAAPAELIVVDDA
                     SGDGTGSRAADLGARVVTHERNQGEGAARNSGIEAARHEWVALLDSDDEWLPEHLETL
                     WAAHDGHVLVGTAILGMGSGANHHRVFGWTGRRPRVLRGPADVAVPDSKLTASSVLVR
                     REAALRAGGFRPLKRASDLDMWLRLLQLGSALAIPRVTALYHVHDDQVSADPRRMHES
                     HKSVLDEHAREPWCTPALRRRHEGVLAWDTARGALAAGAPRWRTLAGLARELAHPQRA
                     AGVAQLLAGRLSRRRAAAAAVRELGDAVRELGSAEPRA"
     gene            25197..26360
                     /locus_tag="VF517_16495"
     CDS             25197..26360
                     /locus_tag="VF517_16495"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8054588.1"
                     /translation="MRAPWGRPPNVEKLARKENVKGLVRALRYEDPISDTGGRTADLG
                     VEVRAAAAAALGHLDTPAANAGLMSALEDPEERVRVAAVRALRERGGTTAVAPLMSAA
                     ASWTDPAREEAREEAVEALASAHHPDVLREMVAALLARPEDLEEPDADVVRRLAEATG
                     APAALRDTIEDLIAHLVEPATAPRARTLLVWLAPESVEPLIAAAGDETAAPEAARALG
                     AIRDSRAVVPLSSLVVSSEDPLVRAAAAWALGEIRDPSAVGALLAATSDGDYAVRAEA
                     LEGFDKLGNAAIAVAMSMLVQPMLENGARRAEEVEGDRTEELAAAAPGELPAEASGAG
                     AAADASGAGPTEPEPSAPVQEPGVAVAAPAAPSPAQQPLATRAAPYIRRLLGR"
     gene            complement(26357..27724)
                     /locus_tag="VF517_16500"
     CDS             complement(26357..27724)
                     /locus_tag="VF517_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054589.1"
                     /translation="MGGPNRAFVVVALVAAALTVAAISSARAPRTVPVDASLPAAGQS
                     DLLARGAVPVELRHRSGGRVRLAAALRGADGSTQALGAARVVTLARGRQRTVKLPLGD
                     AGRALLESCPAGKLLVTVEGKGYSKARRASAPLRLDPPDCERFFSPRAFWNEALPADA
                     PLDPSSAEVTGELIRSVEAGPGQGRPATINTDAYTPPVYTVPADQPRVKVELDQPAGI
                     ADDLRAALASVPLPADARAAPGTDAEIVVWQPSTDTLWEFWQLRRKSGRWHARWGGRL
                     AGVSTGPAVFGSNWGTSASGLPLAGGLITPRELRSGRIDHALALGVPDVRAWEFAVPA
                     RRTDGVSTCRHAVPEGARFRLDPALDVDALGLPPAVATIARAAQRYGIVVREQAGAVA
                     FYAQNASTFRADPYADLFQGRPPWELLASFPWRHLQLTRMQLVGTEGQERPLPRPEDV
                     LRGCT"
     gene            complement(27739..28755)
                     /locus_tag="VF517_16505"
     CDS             complement(27739..28755)
                     /locus_tag="VF517_16505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8054590.1"
                     /translation="MSQPARLAQIDLLKGAAVLGVIALHGLTAHELDDAWEGLHFGQA
                     VPIFLVLMAMNAASSLRRRGDAPLRDLFTRSYFAGRFERLLAPFAVVWVASLVLGLAL
                     SEDVGFGPTTAVGVTPLTGPGNYFVTIAFEFVLVFPLLFLAFRRAPLVTIAACFAIDA
                     AFELIAPHVFSGSYPFVYDAAILRYFGQIALGLWIAEHSGLADRRNRWILGLALASVA
                     YLVFEHQSDGGFDWLRHDFGATTNFLAAPYAAALVLLGLRFLPSRETAAPVRVLAAIG
                     RASWHIFLVQMIWFAVVDARGLDAFVLNAGGACAIGYSLYRVMALPAATARIRRGSAW
                     LARA"
     gene            28821..30194
                     /locus_tag="VF517_16510"
     CDS             28821..30194
                     /locus_tag="VF517_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054591.1"
                     /translation="MPRPLRTLALCVAFAMLFACSGAGPGAGPVQGVEAAAAAKRVPT
                     GVAAKRRAAARRAAAKKKAAAKRKAAAKRKKKKPVKPPRPPAAPSPVVPVAPSPAVRT
                     PAVPTPALPAVPSPAPAAPAPVPPTAPVPAAPSAPAPTLPTATAPAAPAPGAIGALDV
                     VTPFPTGMRLFASSSFWNAPLPADAPLNPASPRLAGALAAEVQREAAARKGPWINTDQ
                     WSVPVYTVGADVAPVRVALDVNKPALQRGFEAVPIPPGARPARGFDGTLVIYQPATDT
                     LWEFFVARQDASGWHARWGGKMTNVSSNPGYFDAPYGGSGTSLALLGGLMTIEEMRAR
                     KIDHALALAIPNTAAGEVVWPAQRGDGTTTGTEAIPEGTHFRIDPRVDLDSLGLTPLG
                     LSIARAAQRYGMVVRDTAGNVALYGEDPVTTTGDPYGEIYAGRYPSDHLRNFPWDRLQ
                     VVAPRGG"
     gene            complement(30199..>30763)
                     /locus_tag="VF517_16515"
     CDS             complement(30199..>30763)
                     /locus_tag="VF517_16515"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8054592.1"
                     /translation="VGVLVAFAAIPGLAGDVNARQNDKTPVYDRENTNAAGLRLVAHN
                     PFVGVGWDRPNAELEPYFRMDETIPLTGGRAGLHNIYLYYVAGLGLIGFGLWLIALLF
                     SFSRALSPSDSLLLVPWKVGLKALLVAWLVVGLFSPAHYPFTTYLVWAWAGVACGTPV
                     GAPALSRFLSRNGGSRAPLAAAGLQSR"
BASE COUNT         4172 a        11255 c        11302 g         4034 t
ORIGIN      
        1 ctccggcctc gaggccggtg tcgtggccgc tgcgggcggc gctctcacgg cggccggcgc
       61 tgcgctgcgc aggctcgtcc ggcgcgggtc agagtcctag acccctcgtc cagccctggc
      121 gctccggcga aagctcgaca tcccggtcgc gctcgcgctt gccctcctcg gggtggcgat
      181 cacgatcttc gcggccatcc ggctgcgcga cacctacgtc ggcgacgccg ccgtctacct
      241 cccgtacgcg cgcaacgcgg gtgacggcca cccattccag ttcaatctgg gcgagttctc
      301 ctccggcagc acctcgccac tctggtcgct gatcctgggc gtcccgttcc tcgcggggtt
      361 cgagctgatg ggggcgaagg cgatggcggc tctgttcgcg gcgatcggct tcctgacgac
      421 gctgctcgcc gcccggcact tcagccgctc ctgggcggcg gcgggcgtgg ccgcgctgtt
      481 cgcgctcggg acgatgacct tccccgcggt ctccctgtac gagtcggggc tggtggtcac
      541 gctgagtgcg ctcgcgctgc tggcggggga ccacgtgctg ggggcctggc gcgcgacgga
      601 cgctccgcgc gcgcccgcct ggcggaccct cgcgccgctc gtggccgtct gggccgcgct
      661 gccgctcgcg cgccccgatg cggtgatcct cgtcggcgcc cacgcgctgg cgctgctcgc
      721 cttcgcgccg gtcgagcgcc gccgcgcgct gcgcaccctc gcgcccggcc tggccgtggc
      781 cgcgctcccg gcgctcgcgt acttcggcta ctcggtcgcc gagctcgaca ccttctcgac
      841 ctcctcccag gcgcgaacgt tcgccctgcg cgagggcgcc cgcgactggc tcggcccgct
      901 cttcctcgag gacggcgcgg tgcgagagct gttcgccagc ccgtggatct tcggcctgct
      961 gccggcgctc gccgggctct ggctcctctg gcggcgggcc gagacgcgct gggccgccgc
     1021 ctacggggcc ctcgcggtgg tcggctacgt gctcctcctg accttcgtcg cgccgggcat
     1081 ctacgacacg ccgcgctacc tgctcccgct cgtgccggtg gtggcctgcg gcgtggccgt
     1141 gctcctggcg cgccccgcgc agccactgct cgcgtgggcc gcgctcgggc tcgcggcgct
     1201 cgcgatcggc ggatccgccg ccgacgagct gcgcgacaac gtgcggctgg cgcgctcgat
     1261 cgggatcgac cgggacgagg tgttcgagcg cgacgtggtc gcccgcatcg acgcgctggc
     1321 cgagcccggc gacgccgtac tggcctacga ggtgcagctc cgctacttcc tgcgccagga
     1381 cgtgaaggtg ttcagccagg acgggatcac cgacccgcgc gtggccgcct accgcgagga
     1441 caaggacatg accggctacc tgatgcgcta ccggccggag ttctggatcg ccgatcgcaa
     1501 cgctcagacc cgccagtaca ccgacggcac gatcctcgag cgtgccctgg tggcctaccg
     1561 gcactcgccg cgccccgcgg tgcgggtgtt cgacgggatc cggttcgagc tggtcgcccg
     1621 gcgcaaccgg ccgctcgcgc ttgggttcgg cggctgggag ttcctcttcc gcctctcgta
     1681 cccgccgccg cgcggagggc gttcgcacct cccctcccag tagaacgaag ttctagaatc
     1741 tcgttctagt ggtggagggt gcgcgcgccg ccctcgccgc ccccgccgcc ctcgccgccc
     1801 tcgccgccct cgccgccctc gccgccgacg ccgccgacgc cgccggtcag ctcgcggtag
     1861 agcgcctcga cctccgcggc gtggtcggcc atcgggcgga ccagctcggc gcggcgctcg
     1921 cgcaccgagc gccgcaggcg ttccacctcg gccgggtcgt cgatcgcacg cgccatcagc
     1981 tccgcgagct ccgcgccgct cgcgctgcgg ttgagccagc cggtctcgcc ctcgcgcacg
     2041 tactcgggga tcccgcccag ggcgctgccg atcagcggca ggcccttcgc gagcatctcg
     2101 atcccgacga agccgtggac ctcctcccac acggacggga cgattcccac gtcggccgcg
     2161 tcgagcagcc cgtcgaggtc ctcgggccgg taggggccgg ccagctcgac cgagccgatc
     2221 gcctccagct cgtcgtggat cgagcgctcc acgccgccgt gaacgaccag ccggtacgag
     2281 ccgccgcggc cggcagcctc gagcgcgcgc accgcgtcga ggatcgcgcg cgagcccttg
     2341 gtctccgagg ccgccgcgcc gagggtcacg aacgtgagcg gcgcgcccgg ctccgctccg
     2401 cgccgcggcg ccagctcggc gaggtgcggc agggtgagcc gctgaaccac gatccgaccc
     2461 gggtcgacgc ccagctccgc gtagatctcc gccacgcgcg gcgacggcgc caccagccgg
     2521 tcgcacatcg agagccggcc cacgttcacg tcgcggcggc gctggaacgg ctccgggtcg
     2581 agcggcggca gcgtcggctc gtccggcacg ggccccgcgg ccggctccac gccgtgcgag
     2641 cgcacgtcca cctcgggcag gccgtgcggc tgcgcgcgca cgagcagccg gttcaccgtc
     2701 cccacgagcg gcgccacgcg cccgaagtcg aggtcctcgc cgcccggcac gagccgcttc
     2761 gcgcgcctgc ggtagtgcgc gagggtcgcc tcgatcaggt ggttgttgcc ctccggtgcc
     2821 cccgcgcagt tgcgcgcgca gtcctcgccg acctcacgcc gcatgcagcg agcgccgtcc
     2881 gcgtcgatca gccgcaccga cgcgcacagc ggcttgtagt cgtgcagcgt catcacgacc
     2941 ggcacgcccg cctcgcgcgc gcgttcgagg atcgacgagg gcagccgcag cagctcctgc
     3001 acgtgcacca cgtcggggcg cgcctcgcgc agcgcggccg cgaacgccgc gtccccgacg
     3061 ggctcgtcga tgtcgcgctc cgggtgccgc gtgccgcgct cggtgtgcgt gttgatcggc
     3121 gagccgatca gctcgaaggt gcgcaccccg cgccggcgcg agcgcttgag ccgcgggcgc
     3181 tcgagtcgcg ggaagtagcg gcccgagaac aggtacgcga cctcgtggcc ggccgcgcgc
     3241 tgggcgtcca tcagggcctc ggcatagaac gtcaggccac acggccgcag gacggcgttg
     3301 tcgtccccga cgtgtagcac tcgcaccggg gccaatgtac agttccgccc gtgagcggct
     3361 cccacatgga gcgcttctgg gacgcccgtg ctcgcgagga cgccctctgg tacgtcgaca
     3421 gccggctcga gttcggcgac ggcgacgagg aagcgttctg gggagggggg gaggaggcgc
     3481 tcgaccgcct gttcgaggtc ctcggtgcgc gccccgaccc gggcgctgtg atcgtcgaca
     3541 tcggctgcgg cgtcggccgc ctgacgcgcc cgctggcacg cgacgcccag cgcgtgatcg
     3601 ggatcgacgt gtcgagcgag atgctcgacc gcgcccgcga gctcaacgcc cagctcgaca
     3661 acgtcgagtg gctccacggc gacggcgagt cgctgcgccc gatcgaggac gcgagcgccg
     3721 acgtgtgcgt ctcgcacgtc gtcttccgcc acatccccga cccggcgatc acgctcggct
     3781 acgtgcgcga gatcggacgc gtgctggggc tcggcggctt cgccgcgatc gagttctcga
     3841 acgatcccga gccccacaag cgccgcgcgg tgcgcgacgc gaagacgaag ctcgccgccg
     3901 ccgttggccg caagccgcgc ggcatcgcgg acgccgagtg gctcggctcc tacatcgacc
     3961 tggccgacct gcgcagagcc gcggccggcg ccggcatgga ggtcgagcgc gttgtcgggg
     4021 agggcaccga gtactgcgcg gcgctgctcc ggaagggagc gccggcatga gcacggtcga
     4081 caactactac gaccagttct ggtccgggga gaaggagcgc cactaccagc cggagagcgt
     4141 gctcgaggag ctgatcctcg agggcgtgac gagcaccacc gcggtgctcg acgtgggttg
     4201 cggcagcggc aactcgtacg cgggggaact gaaccgccgc gccgcctcgt acgtcggcgt
     4261 ggacgtgtcc gccaacgccg tggaggccgc gcgcgggctc gggctcgacg cccggaccat
     4321 cgacgccgcc gcctcgctcc cgttcgagga cgagagcttc gacctcgtcg tctgcatcga
     4381 ggtgatggag cacctgttcg cgccgcacga ggccgccgcc gagatccggc gcgtgctgaa
     4441 gcccggcggg cggctggtct gctcgacccc gaacgtcgcc tactggcggc tgcgcgcgaa
     4501 catggtcttc ggcctctgga acccgctcgg cgacgagctg gcgctcgagc gcccgtggcg
     4561 cgacccgcac atccgcttct tcacgcccaa gacgctcgcg cggatgctgc ggatgagcgg
     4621 cttccgcgac gtgaggaccg gcgcgcacgg ggggcgcttc ctcgaccacg cgagctcgcg
     4681 ccccacggac ttcggtcagg ggcgcgctta cgcggcgatc gagccgctcc tgccgtcgct
     4741 gctcggcctg acgatccacg cggtggcggt caaatagcga gccggctact cgaccccacg
     4801 ttcaggatgc gctggttcgc gacggccacg cggccgtact ggcgcaggcg cttcgcggag
     4861 ttcggcagcg agtcgctgct ctaccggccg gcgtgggtgc accggccgga gctgatggcg
     4921 atcggtagcc gcgtgtggat ctcgcaggac gcgtggttcg aggtgggcgg cgagcccggc
     4981 ggcccgccgc gcatcaggct cggcgaaggc gtcgtgctgc gccaccacgt cacgttgtcg
     5041 gcgttcgagt cggtgacgat cgagccggag gtgctcatcg ccgcctacac gtcgatctac
     5101 gactccgacc acacgatcgg ccgtgatcgc aacgcgatct ggtacccgca ccgcgtcgag
     5161 ccggtccgga tcggcttcgg ctcgtggctc ggcgagcgcg tgcaggtgct caagggggcc
     5221 gacgtcggcc gtcactgcat catcggcgcg aacagcgtgg tgcgcggcac gatcccggac
     5281 tactcggtgg ccgtgggcgc ccccgcgcgg gtggtggggg acacgcgcga gatggtcgag
     5341 cgctccgaac cttgatccgg gggctgctgc gcacaccgcc gcgcccgctg tccgggccgg
     5401 tgcgcccgcc gctcctcccc gctgccgcct acgtcgtggc catgacggcg ctccagatgc
     5461 tgcgccggcc gcacgaccca cccgcctggg actcgctctt cgtcgaggac ggggagatct
     5521 tcctccaggg tgcgatctcg cagggcctcg tcagctcctt caccaccgcc taccagggct
     5581 acctgcacgt cgtgccgcgc acgatcgccg aggtggccgg gcagctcccc ctcgagcgcg
     5641 ctcccctcgt gatggcgctg ctggccaccg gcctcgtgtc gctgctgtcg gtctatgtgt
     5701 attgcgcgtc ggcggcgtgg atcggctcgc gcgtgctgcg cggcgcgctg gcgatcgcgt
     5761 tcgtgctgct tccggccggg ctgctcgaca tgaacggcgt cacgtcgtac gtgagctggt
     5821 acatgctcta tgcggcgttc tgggcgctcg catccccgtg gctcacgcgc ggctggatcg
     5881 ccgtctcgtc ggtgctggtg ctgctcaccg cgctcagcaa cccgctgctc gggttcttcc
     5941 tcccggtcgc gatcgtgatg gcgctgcgat cgggggagcg cagggcgtgg atcgtccccg
     6001 gcatggcatc ggcgggactg ttcgtgcagc tcctcgcacg cgaacccccc gagacgttcg
     6061 gcgagacgat gcccggcgag atcccgcgga tcttcgccga ccgcgtgccc acctcgctcc
     6121 tgaccggaga gcgcttcctc gaggacctgc tcggcggcac gagcggctcg ttcttcgcct
     6181 gggcgtcgct ggcggtcgtc gccgccgcgg tcgggttcgg cctggtccgc ctgcggggcc
     6241 gccgcgcctg gctgctcggt ggctgcgccc tcgcggcggt cgtgatcttc accacggccg
     6301 cgctgatccg gggcaccgag cagttcctgc cggacgaccc gtggctcggg ttcgcctcgc
     6361 gctacgtgta tcccgcggtc ctgttcctcc tcaccgggct gctcgcagcg gtcgaccgcg
     6421 ccgaggccgc gccacgtctc ggaacgccgc gcgagtgggc cgcggcggtg gcgctgctcg
     6481 cggtcatcgc cgtgaacctg ccggtcgacc accacgccgt ccgtggaccg cgctggggac
     6541 cgcagctcga ggcggcgcgc gagcgctgcg accgcccggc gcgagagcgg ccgcgcgagg
     6601 tcgtgatccc cacggcgccg tacacgccgc cggggaccga gcggggcccg ttcacatggg
     6661 aactgcgcgt cagctgcgac gcgctctagc ggcgcgcgcg gcatcgcgaa acagctcgac
     6721 gtagcgcggc cgcaggccct cccacgagta cttctcgtag gcgtagcggt gccgcgcacg
     6781 ctgagcgtcg gggctgccgt cgaatccggc gcccgcgatc agcccggcga gcccgccggg
     6841 gcgcgagaag tcggcgaagt ggccgtgctc gccgagcgcg tagcgggtta cgccgtagtc
     6901 atgggtgagg caggggagtc cggccgccag cgcctcgatg agcgcacgcg gcagcccctc
     6961 gccgagcgac gcgagcacga actcgtcggc cgaacgcagg agctccggca cctgccgctg
     7021 cgggaccgtg cgcatgtcgt gcccgtccgg tccgagccgc tcgcgcgcga gcgctcgcag
     7081 gccctcggtc tcctcctcga cctggcccgc catcagcacg taggggcggt cggcctccgg
     7141 cagcgacgcg acctcctcga tcaggtagtc gagccgcttg tggtggcggt tgagcgcggc
     7201 ggagctcagc aggatccgcc ggtcgagcgg cagccccagg cgttcccgcg cggcgcgcct
     7261 gtcgtcgtcg gtgacgggtt cgacctgcgg atcgatccag aacccgagcg ggagcatcac
     7321 gtggcgctcg gggtccgcgc cgcgggcaag aatctcctcg agcgccgccg ggctgagctc
     7381 ctgcacccgg tcgaggtggc cgaacccctc gagcgccatg gtcccgttcg agagcaccag
     7441 tgcgaagctg cgtccggcag catcgcgcag cggcgcgagg accttcccgg tgtgccactc
     7501 gctcaggaac acgacgtcgg ggcgctcccg cagcagcacg ggcatgacgc tgaacgcgaa
     7561 cgcgacgtgc tcgaacctga acgcctgacg gccgctgagc cgcgcgagcg ccctgaccgg
     7621 cgccgagtcg cgcgtgagcg tcggcaccga cctctcgcga tcgcccgccg cgccgcttcc
     7681 cttgacgagc ttcatgtcga gcgcggggtc gtcgcgcagc gcgtcgaagc actcgcgggc
     7741 gaacgactcg aagccccggt gggcgtggtc gagccccgag cagatcagca ggacgcgtag
     7801 gggtctggtg ttcggcatgc agatgcggcg ggggacggta acgcgcccat aacatgaccg
     7861 cgatggcgct cgcccaggag gacgcaccca ccaacctgcg tgcccgcacc gcgcgcggca
     7921 cgctcatcaa cgcgatcttc atggtggccg tcagcatgct cgcgctcgtc caaggggtcg
     7981 cgctggcgcg gctgctctcg ccggagacct acggcctctg gggaatgttg atggcggcgt
     8041 tcatcacgct gctcacgctc ggctcggtcg gcatcgacga caagtacatc cagcaggacg
     8101 acgaggacca gcagcgggcg ttcgagatcg cgttcacgat ccagatcctg ctcgcgccgg
     8161 tgttcgtggt gctgctgctg atcgggatcc cgctgctggc cctcgtctac ggcgagacgc
     8221 gcatcatcgg gcccggcctc gcgatcgcgt tcgtgatccc ggcactcgcc ctccagatgc
     8281 cgctctgggc gcactaccgg cgcatggact tcctgcgcca gcgcctgctc cagtcgatcg
     8341 acccggtgat gacgttcatc gcgacgatca cgctggtcct ggcgggcctc gagctgtggg
     8401 gcctcgtgct cggcgccatg atcggctcgt tcgccgcgag cgccgcgatg gtggcgacgt
     8461 cgccgtacaa gctgcgctgg cgcttcgacc gcgacgccct gcgcgagtac cgcaagttct
     8521 ccacgccgct gttcgtcgcc gcgggcagca ccgtgctgct ggtgcaggtg ccggtgatcg
     8581 tcggctcgaa cgtgctgggc gtaacggccg tggccgccat ggcgctggcc ggcaacatct
     8641 cgcagttcac gaacaaggtc gaggatctgg tgaccgggtc catgtacccg gcgatctgcc
     8701 gcgtgaagga ccggaccgac ctgctgttcg aggcgttctg gaagtcgaac cgcctggcgc
     8761 tcctgtgggc cgcgccgctc ggcgccgccg cgccgctgtt cgtcggggac ttcgtgcacc
     8821 acgtgatcgg cgagaagtgg cgcttcgcgg cgccgctgat cgggatctac gggttcctcg
     8881 ccgtcttcag ccagatcgcg ttcaactgga ccgcgttctt ccgcgcggtg ggcgacacaa
     8941 agccgattgc caccgcgagc gtcgtgaaca ccgccgccgt gatcgcgttc ggggtgcccc
     9001 tgctggtgct cgagggagtc accgcgttcg gcgtcggcgt ggcggtcgcg acggtgatcg
     9061 gcgtgggcct gcggctctgg tacgtgcgcc gcctgttccc cgggatgccg ttcgtgagcc
     9121 acatcgcgcg cggcatcctg ccgaccgtgc cgggcgtggt ggccgtgctg gcgctgaggc
     9181 tggccgaccc cggcggccgg acggtcgggc gggtcgcgct ggaggccggc ctctacaccg
     9241 tgctgatcgt gctcaccacg tggttctccg agcggtcgct gctgcgcgag tcggtctccc
     9301 tgctgcgagg aggcgcgcgc ctgcgcgagg ccccggtggc gtgagcgcga tcgggatcgt
     9361 catccccacg ctcggccgcc acgcgctgct gcggcgggtg ctcgaccggc tcgaccgcca
     9421 gaccgcgccg caggggtcgt tcgaggtcgt ggtcgtggcg gacgcgaagg aggagcgcct
     9481 cgacgagatc gaccgggcgc tcgagggccg tagcttcccc gcccggcggc tgcgcgggcc
     9541 gagaccgggc gcctcggcgg cgcgcaacga gggctggcgc gcgctcgact cgccgctggt
     9601 cctgttcgtc gacgacgaca tcctccccga gccggagctg gtcgcccagc acctcgggtg
     9661 gcacgaccgc cacccgcagg aggagatcgg cgtgctcggc cacgtgcgct gggccgacga
     9721 gctcgacgtc acgccgttca tgcgctggct cgagcacggg atccagttcg actacccgaa
     9781 catcgacggc gaggacgccg gctgggcgcg cttctacacc gccaacgcgt cggtcaagcg
     9841 cacggtgatc gagcgcgtcg gcggcttcga ggaggagcga ctgccgttcg gctacgagga
     9901 tctcgacctg gcgctccgga tgcacgagca gaccgggttc cacctgctct acaaccgcgc
     9961 ggcggtggcc gagcacgtgc acccgatgga tctcgagttc tggaagcggc gcgtggcgcg
    10021 gatcgcgatc tccgagcgcg agttcgtgcg catgcacccg gacttccgtc cctacttcta
    10081 cgagctgttc tcgaaggcgg cgtcggaccc gcgcgccaac ggccgcggcg agcgcctcgc
    10141 gcgcttcgtg ccgcgctcgc tcccggtgct gggcgagcgc gtgtggagca gcgccgacgc
    10201 cgtctaccgc caggcgctgg cggggccctt cctggaggca tgggaggcgg ccgggggctg
    10261 acgccagccg tggcgcagcc gcaggaacgg ctgctccacg aagcggtagc tgagcgcggc
    10321 cagcgcggtc gcgagcgcga acgcgccggc gatcaggatc gcccgccggg cgccgtggct
    10381 cacgtcgatg cggtcgtcga tccagagcat cgcgccgagg tggaacaggt agatcccgta
    10441 tgagcgctcg ccgagccact ggatcggccg gttggtgagc gcgcgccacg cgccgccggt
    10501 ggtccactgg agcatcagcg cggagccgac gatggcgccc gcggcgagcg ccgccgtgat
    10561 cgacggcttc caggccgagt cggccgggag ctggaacgtg tggaccgtcg cgatcgcgag
    10621 gccgccggcg agcagcgcgg tcaccagcac gcgcgcgccg cgcaggccgc gcaggcgcgc
    10681 gccgaactgc acctcgacca gcgcgagcac gaggcccggc acgaacgcgt agaagacggc
    10741 cggggggctg cgctgccagc cgtcgctcag aagcgtgttc gactcgagcg cacgctggaa
    10801 ggcgaggctg gcgagccaga cgcccgcgag caggagccag accaggccgg cgcgcggcac
    10861 ccgtgtgcgg cgggcgatcc agaagagcgc cgccgccgcg agcgggagga ggaggtagaa
    10921 cgccagctcc acgtccaccg accaggcgtg cagcatgtgg cggctgaacg cgctcgaatc
    10981 ggcctcctgc acgaacagga agacggccgc gatctggccg aggccggcgc cctggcggcc
    11041 gaagacgatc agcgtgagta tcgccgccag ccacagcgcc ggcacgatcc gcagcaggcg
    11101 gttgcgcatg tagttgcgga ccgacggcag ctcgcggcgc tcgacgaacg ccgcgacgaa
    11161 cgggcgcgcc agcaggtagc ccgacaggac gaagaagacc cacaggccga tccgcccgag
    11221 gttggcgatg cgcgcgtcga tcgagtcgtc gggcgtcgcg tccgggtggg tcgtggaggc
    11281 gacgaccagc gcgtggaaga cgagcacgcc gacggcggcc aggaggcgga ggccgtcgcc
    11341 ggccaggaag cgatcgagcc gcggtgccgc gtcggacggc tgggtcgcgt gctcgcgggc
    11401 ctcgggtgga gcgtcggccg cgccgtccgg cgcgccgagc ctcacgcggt ggtgctccgc
    11461 agctcctccg gctcctggcc ggggggcccg aagtagtcga tcggcttcgc gggtgcgccg
    11521 accgcgacgc agtagggcgg gatcggcttc gagaccaccg cgttcgcccc gatgaacgag
    11581 cgcgttccga tgtcggccat gatcgagcac ttcgtcgtga tcgtggcgtg gtcctcgatc
    11641 ttcacgtggg cgaagtcgta gccctgctcg agcatcggct tcgtcaggtc gcggaagcgg
    11701 tgctggccgt cgaccacgat ggtcgactgc ccgaactgca cgtgctcgcc gatctcgatc
    11761 agcgtcgagc actgcatcag cacgtagtag gtcatgtgcg agtgcgagcc gatcgtgatg
    11821 cggccgtcgc catccacctc ggcgcggaag ccccgccgga actccacgcc gtcgccggcc
    11881 tcgaaggtgc cgcccgcggg gatgtggagg ctgaagccgg gaccgaggta gcagccgtgg
    11941 ccgaagctga tgttcgcctg cggatggcgc aggatcaccc actgcatgcg cagccacgac
    12001 atcagccgcg ggccggggcc cgggtagtag ccgagccgct gcggcagcgg gcgcaggcgg
    12061 tcgcgcactc tcggggggag caccgtcttc aagagctcct tcacgcggcc accggctccc
    12121 acgcgtggcc ggcgacctcg agcaggtgtc cctcgacctg ctcgttgaag acggtctcgg
    12181 tatggagcgg cgccgtgcgc tcgccgcccg cgcgcagggt gcggcgcagc tcggggtcgg
    12241 ccgccaggcg ctcgacggcg cgcgcgatcg cctccgggtc ccgcgccgga acgatcaggc
    12301 agttctcgcc gtcgcgcatg tactcgccgc cgccgccgcg gccggtggcg accacgggga
    12361 ggccctgcgc catcgcctcg atcgggacga gtccccacgg ctcctcccac aagaccggga
    12421 acagcagcgc gtcgtgggcg cgcatgagct cggcgacctc cgccggcgag cgctggccga
    12481 gcatcgtcac gcgcgactcc accccgagcc cgcgcgcgcg ctccaccagg gcgtcctcgt
    12541 cgcgctcgtc ccacttgccg gcgaacgtga gggtggcctc cccgggaagg tgagcgagcg
    12601 cgtcgaccga ctcgtggatg cccttgtcgt ggtggatgcg cccgacgtac agcagccgcc
    12661 agctccagtc gtgctcgggc ggcagtgggc cgacgagcgc cgggtcgatg ccggagtggg
    12721 ccacggcggt gtcgcggacc ggctcgggca gcttcagcgc ccgccggcgt acgaagtcgc
    12781 tcacgaacac gtagcgcccg gcgcccgcgt agtcgacgcg ggtggggatg ccggtgaggc
    12841 ggtcgaccgc acgggccgcg agccggcgtc gcggagcgtg gaagagccgc atccactggt
    12901 cgaaagaacg gccgtagtcg agccactggt catgcacgaa cgcgaccatc gggagcccgc
    12961 gccggcgcac gcgctcgatc agcgagtgcg acatgccgcc catcgaccag aagctcacga
    13021 cgtcgggctc gagctcgtcg aggtggcggg cgagcacgtg cgcgttgtgg cgctcgatcg
    13081 ggggacgctg cgcaagcggg atgcgcggaa agtcgtgccc agcccagtac cagcgcagct
    13141 cgcggtgcac gtcgggctcg tcgccgtcgg cgacgccgtc gaggcgccag tcggtggcga
    13201 gcacgcgcgc ggtgtggccg ttggcgcgga ggtgcctcac ggccgacctc catacctgtt
    13261 cgtagccgcc gaagtggtgc ggcgggtaga ggttccccac cgtcaggacg cgcaccggcg
    13321 gcactctagt gacggatacg ctggcgccgt gccgccacgc gtggtcatag ccgtcgtgtc
    13381 ctggaacacc cgcgagctgc tcgaccgctg cctgctgtcg atgaagccgc acgcggacgc
    13441 cggggccgcg tcggtgtggg tggtggacaa cgcctccacg gacggctcgc cggagatggt
    13501 gcgcgagtgc cacccgtggg tcgagctcga ggcccgcgac gacaacctcg gctacgggcg
    13561 ggcgctgaac ctggtggccg agcggaccga gtcagagtgg ttcgcgatct ccaacgccga
    13621 cgtgcacctg cgcgagggcg cgctcgagcg gctgctcgcg gcgggcgacg ccgacccggg
    13681 ggcgggcgcg gtggcgccgc ggctgatcct gcccgacggc tcgacgcagc actcggtctg
    13741 ggcgttcccg acgctcgcgg ccaccgccgt gcagaacctc ggcccgcggc tcagcccggg
    13801 cgacctggcc gagcggatgg cgatccgcgg cagctggaac cccgaccgcg ccgggcgcgt
    13861 gccgtgggcg atcggcgcgt tcatgctgat ccgccggcgc gcgtgggacg acgtgggtgg
    13921 cttcgacccg ggtcagtgga tgtcggcgga ggacctcgag ctgggctgga agctgcgcga
    13981 gacgggctgg gcgacgtact acgagcccgc cgccgtggtc gaccacgagg agtcggccgc
    14041 gaccggcgag gtgtggggcg aggacctgcc gctgcactgg cagcgctgcg cctacgcctg
    14101 gatggtgcgc cgccgcggac gcgcgcggac cgcggccgtg gggacgatga acttcgcggg
    14161 gtccggggcg cgctgggcgc gcggcatggc gcgggcgcgc ggacggcccg accccggcct
    14221 gcgcgccctg ggacgctgga cgctggtgca ccgctacgcc ttcgcgcccc ggcgcgtgct
    14281 cgggaggtac cgctgatgcg cggggccgag cgccttcgcc gggtcgcccg gcgcgcttac
    14341 tactcgcgcc gcggcgtgcg cgagctggcg gccctcgggc gcctgccgct gctgcgccgc
    14401 gagggccccg acctcgccca cctggcgttc tacgaggaga tcgccgaggg cccggtgcag
    14461 cgcgacgagg cgctgttcct gcacgcgctg atccgcgtgg tccggccgca gaccgtggtc
    14521 gagctgggct tcctccgcgg ccacagcacg ttcaacttcc tgcgcgcgct cgaccccggc
    14581 gcgcgggtct actcgttcga catcgacccg gcgtgcgagg acttcgcccg ggagcgcttc
    14641 tcgcacgacc cgcggctgac ctaccgcacg aagtcgcagg acgagctgac ccccggcgac
    14701 gtcgacggcc gcagcgccga gttcgtgttc atcgacgccg cccacgagct gtcgctcaac
    14761 caggccacct tcgagcggct gctgccgctg ctcgcccccg acgcgatagt ggcgatccac
    14821 gacaccggca cggtcccgcg ccgcttcgtc gcgccggacc acttcaccca gggaatccct
    14881 cagcgctggg tcggcgacgc ggtcgagcac cagcccgacg agcgcgcctt cgtcaactgg
    14941 atcctcgccg ctcaccccga gttcgcgcag ctgcacctgc actcacggca cgctctccgc
    15001 tggggtctca ccctgcttca acgcagcgcg ccgttgccgc gaccggccgg gatctaggtc
    15061 ctcggccatc ggccgcttgc tcacccgccg ggccttgaac cgtagggccg ggcgctcgac
    15121 gaggaaccac gacccggccg cgcacaggac cgtgatcggc agggccatcg ccagcagccc
    15181 cagcggcgag agcccgcgcc cccacaggtg ctggaccgtc tcctgcaccg gcgagcccca
    15241 gatgtagatg ccgtacgaga ggtcgccgcg gtcggtgagc agctggatcc gcttgggggt
    15301 gttgtaggcg aggaagatcg tggtgtacgg gatcgcgagc gccgcgagca gcgtcgtcgg
    15361 gctaccgccc gcggcgggtg cggcatcgcc gccgacgacg agccacgcgg cgaccagccc
    15421 ggccgcgatg gcccagtgcc agggcacgat ctggcggaac acgtagagaa acgatccgat
    15481 ggcgaagcac tgcacataca gcggcgcgac catgagatcc ccggcacgct ccggcgccag
    15541 ctccgggccg acgagcagca cggccagcag cccagcggtc aggacgcggc tgcgcaggaa
    15601 gccgacaaga ccgacgagag cgagcatcgc gtacgagcgc acctcgggcg acagcgtcca
    15661 gagcggaccg ttcacggcga gcgggatcgg gttgtccggg aagacgccgg gcagcgagta
    15721 tcgcgtctcg aagacgaggt tgttgtacag gtagccccag gtctccgggt ggctcagata
    15781 ctcgcgcacc ggcagtgtcg tgacgacggg gccgagcacg aacgtcgaca gcacgagcac
    15841 gaggatcagc gccgggtaga tccgcagcgc tcgcttcgcg aggaagcgcc cgaggcgcgg
    15901 ctcgagcgtc cagctctgcg cgagcaggaa gccgctgatc acgaagaaga ccatcaccgc
    15961 cgcggcgttc aggcccagcc cgtgcacgcg cggaaccgtg ctctggccga cgaccggcca
    16021 gcagtggagc accagcaccg tggtcgcggc gaccaggcgc agcgcgtcga agttgttgcc
    16081 gcgggaggtc ccgggcttga ccgctcgtgc ggagagcatg ccctccccct tgggctatgc
    16141 cggggccgga agctcctcga gcatccgcgc ggcggtgtcc agcgcctgtc gcatgcagtc
    16201 gtccatgtcg atgtatacgt agttggacag gcggccggcg tagaagacgt tcagcgcgct
    16261 caggcgctcg gtcaggtcgg cctggtagcg gttgttcagc tccctgttct cgggcagctc
    16321 gatggggtag tagcgcgtcg gcgcgccggt gaactcgaag ccgagcacgg tgccgtcgac
    16381 cttctggcgc gacgcgtgct tggtctcgtg gatccggatg aaggggtact cgagccccgg
    16441 gtagttcacg accatcgcgc cctgggcgta ctccatgtgc ggcacgtaca cgctgcgcac
    16501 gaggatcccg cgccagtcga gcctgccgag cttgaagccg gagaactcgt cgagcgggca
    16561 ggtgagcacc accgcgtcgg gcttgacgcg cgcggcgtgc tcctcgacgt tctcgagcgt
    16621 cacgtcgtgg ccgtactgga cctcgatgcc gtcgctcgcc agcagcccgt cgagcaggtc
    16681 ggtgtagccg ttcggccccg acggccagcc ctggtagggg tcggggaaga ggtacgggtc
    16741 gttgtcccag cgcaccgaca cgcgccgcgg cgcccagccg gccgacagct cgcgcggcgg
    16801 gcggccccac tgcttctgcg tgtacgggcg gatgtagcgc tcgtacagcg tcgggcccat
    16861 cagctcgagg caccactcct cgaagttcgc cgactgcgcg cggccggcgg cgtcgacgtc
    16921 gcggcgctcg tcgagctcct tcttgatctg ctcgctcttc gactgggcgt cgatgtcgct
    16981 cagcaggatc ggccagttga ggatcttgcc ctcgaccacg atgtccacac ggtggcggta
    17041 gtcgtggaac ggcgtcatct cgttcgccag gtcccagacc tccttgtcct cggtgtggaa
    17101 gatgtgcgac ccgtgcggct cgtagagcac gccgttcatg tggtcggaac gcacgagccc
    17161 accgtggacg tcctcgcgtt cgaggatcac ggactcgatc ccctcacggg cgaggcggtg
    17221 ggcgacggtg caggctgtga gccctccgcc gacgatcatc acgcgaggca cggcgcgcga
    17281 tcctaaccgt ctagagcatc agcgtgcgat gctcgatgct gcctgcaacg gctgtcccaa
    17341 tctcggtgag gtcgacggcc aggtgggtcg gcaacgccct cgccccggcg ctcgggtcgt
    17401 gcgcgtagag gcggcggtag tggtcgaggt aggggagggc ggccagcgcg gcgaggagcg
    17461 gccggcgggt ggcgaggccg gccagcgcca gcagcagccg cgggtgggtc ggcttccaga
    17521 agacgcgcgc cacgagcagc tcacgcagct ccgggtgggc acccacggcc cgcaccgagt
    17581 cggtccactt ggtggtgtgg cgaatcaggt cgcgcagacc gagccggcgc acctcgtggt
    17641 ggacgagcgc tccggcggcc cacagcgccc gcgctccggc cgcccgcgcc cgcgccccca
    17701 tgtccacgtc ctcggcgctg cgcctgaagg tctcgtcgaa gccgccgagg cgctcgagca
    17761 gcgggcgcga gtaggcgatg ttgcaggtcg cgaacaaccg gtcggggcca tcgacgacga
    17821 tcgtgtggct gagcggggtc agctcggcga gctggttcgg cggcggctgc accggcccct
    17881 ggatcacgac cgcctcgccg gcgtccgtgg ccgcctccac gagcgccgcg agccagccgt
    17941 cggtgggctc gcagtcgtcg tcgacgaaag cgaccagcgg ggcgctcgcc gcccgccagc
    18001 cgctgttgcg cgcggcggcg ggcccgcgtg ggcgctcgtg ccggacgacc cgtagcgcga
    18061 cgtcttccgc cgccggccgc gctggcgggt cggacccgtc gtcggccacg atcacctcga
    18121 agcggtcgtg cggcagggtc tggcgcccga gggccgccag caccgtggcg aggccatccg
    18181 ggcggttgtg ggtcgggacg acgacgctta ccgcgggcgt catccgtcgt tgcgcgccag
    18241 tgccaggatc gagaccccga acgggaggtc ggtacgcccg acgagccggg cctcggaggc
    18301 gaagacccgc cccagcagcg agttcacgcg ccccggcttg gtcagcgtga agtccgactc
    18361 gagctcctcg gccggcttgg ccgggcgcag cacgcggatc gccgctatcg gcgggaacag
    18421 cagcgtgttg aagtaggtcg agcgcagcac ggtgaggccc gcctcctcga aacgcgcggt
    18481 cagctcccgc ctcacgtagc ggcgcttgtg gtggctgatc tcgtcctgcg ggccccacag
    18541 gaagcggtag gccggcacgc tcaccatcac gtgcccgccg ggagcgacca cgcgccggaa
    18601 gtcacgcagc agtccgaggt cgtcgtccac gtgctcgatc acgtcgaacg cggtcacgag
    18661 gtcgaaggtg tcgtcgccga acggaagcgg cccgtcgtcc agcagcccga cgtcgtcgag
    18721 gccacgctgg tggcagaagg cgacggcctc ctcgctcgcg tcgacgccct gcgccgtgcc
    18781 gaagcggccg aggtgggcga gcatcgtgcc ggtgccgcag ccgacgtcga ggatcctgcg
    18841 gtcccggccc ggctccaggt ggtcggcgag cacgcgcatg agcacctcac ggcggccgac
    18901 gaaccaccag tggcggtcct cgatctcgta gtactcgctg tagaaggtcg tgtccacggc
    18961 tactcggcgc ggatcagcgt gatcccgggc ccgtcgggga agtgccgcgc catcgccagg
    19021 cggcggcccg cggggaggtg gttggcccgc gcgagcgccg gcgtcgggtc ggacgtgact
    19081 atccagactc gccgcccgtc cgcggtgagc cgttcggcgc gcgggtccga ggccgccacc
    19141 acgggcgcgc ccggcggcag ctcgcgcgtg gcgtagaaca tgagcggcag gtccaccccg
    19201 aagctgccgc tctgatcgac cacggcatcg ccctcccgcc agtcggaggc gatcgtctcc
    19261 atcgccccgc gcgcgtcggg gaagagtccg tcctcctggt gcgtgcgcac cgtgcccagg
    19321 accgcggcga ccagggcggc ggcgaccagc gcggcacgcg gcacgcgtgg cgcggcggcg
    19381 atcgccaccc cgatcatcac gatcatcgtc ggcaccgcga cggccgcgta gcgggtggcg
    19441 accatgtcct tgcccgcgat gcccgcggcg agcagcccga gcggcgcggc gacggcgatc
    19501 agcagcacca gctcgtggtc gcgccggcgc tcgcgcacga gccgccacgc gagcacgccc
    19561 gccgcggcca tgaccacgag cgcgccgagg atctgcacga tcgtcgcctc gtcggccgtg
    19621 cgtatgtcga acggcgagcc ggtcacgcgc acggcgtgcc cgcgcgagag ctcaccgaac
    19681 tctcccagcc cgccgttggg gtggaggtcg tactggtgga gcatgagcgg cacccacgcg
    19741 aagaagacca gcgccggcac cgcgcagaag accgcccgtg cgcggagccc gaacgagctg
    19801 cgcaggagca cccaggcgca cagcggcccc acgaccagca gggccacgta gtgcagcgag
    19861 accgcaccgc acgcggccac gccggccgcg atcagccagc ggcgcgagcc gcgctgctcg
    19921 gcctcgacca gcgcgagcac ggcgaacgtc gcgacgagca tcagcagcgc gtactggcgc
    19981 gcctgctgcg cgtacccgag caccagcggg ctcagagcgg tgagcagccc cgcggcgatg
    20041 cccgcccagc ggccgccgag cagcacgccg agccggtaca cgacgccgac cagcgcgacg
    20101 gcgaacacca ccgagagcag ccgtgggatc cagtcctcgc tcgagccgaa cagctcgttc
    20161 cagaggttca gcacgagcca gtaggtcggc gggctcgtct cgccgcgcgc gaccccgtcg
    20221 aagaggtcgc cgaggtcgcc acggatgaag cggtgggtcg gcacctcgtc cacgaacagg
    20281 ctggacgagc cgagctgcca caggtacagc gcggcggcga gcgccatgag cccggccgcc
    20341 tccgcccagc gtgtgcgagc gggctcggcc ccgctcggtg cctcagctgc caacggccgc
    20401 cgggacgtac tcgctcgtcc gcgcgcgccg gtgctgctcg tgcgcggcgt actgctcgag
    20461 ctgggcgcgc gtggggaagt agatgcgctg ccacatgcgc agcacctcgc gcagggtgtt
    20521 cggtacgtcg ctcgggcgca ctgtcgtggc gccggccagg cgcgggtagt ggcgcacgcc
    20581 cttctgagcg atgcggtagt tccactttcg cgctcgggcc accagctccg tgtcgatgaa
    20641 gaagttgtcg cactcgaggc tgaggtggtc gagcacctgc cggcgcatca gcttgaaggc
    20701 gcagtccacg tcgcggaccc gcgtgcggaa cagcaccccg acgaggcggt tgtagaccca
    20761 cgacatgatg ctgcgcagca cggtgtcgta gcggtacacg cggaagccga tcaccacgtc
    20821 gtgctcctcc acgaacggga ggaagtactc gagctcggcc acgtcgaact ggttgtcgga
    20881 gtccgtgtag aagacgaact cgtagcggga gcagtcgaag cccgtccgga tcgcggcgcc
    20941 gtagccgatg ttggtctcgt ggacgatcag ccggacgcgg tccccgtgct cgtccacgag
    21001 tgagcggacg acatccgagg tgccgtcgct gctgccgtcg tcgacgacga tcacctcgta
    21061 gtcctccgcg acctgctcga ggacgcccag cgcgcgctcc acggccgtcc ggacgttctg
    21121 ctcctcgttg tacgcaggga ggacgatgga caggccccgc accatggcgc gcgatgctaa
    21181 ccgaagcgtc actggcacgc ccccgctaga ttgccgcgcc gtgcccctgc ccacggtctc
    21241 gatctgcatc ctcgtctaca accgccgcga ggagctgcgc gagacgctcc agcgcatgct
    21301 ggagcagtcc gactacccgc gtgagctgct cgagccgatc gtggtcgaca acgcctcgag
    21361 cgacggctcg ggcgacatgg tccgcgagga gttcccggac gtgaagctga tcgcccgcga
    21421 cgagaacatc ggcgccagcg cctggaacga cagcttcagg atcgcgaccg gcgactacgt
    21481 gctcgtgctc gacgacgact gctacatgcc gccggacggc ctgacccgcg ccgtcgccgc
    21541 cgcgcaggag cacgaggccg atctcgtgtc cttcgcggtc gagagcagcg tgatgccggg
    21601 ctactacttc agccgcgcct acaagaccgg gctgttcagc ttctggggct gcgcctggct
    21661 gatcaagcgc tcggtggtgc aggagctcgg cggctacgac ccggagatct tcatctgggc
    21721 gaacgagctc gagttcacga tgcgcttctt cgaccacggc tacaagcacc tgcacgaccc
    21781 gacgatcgtc gcccagcaca tgaagcaccc gggcgacgac acgctcgaca tcgaggagcg
    21841 cggctacaag atcaacgcca accactgggg ctacatcgcg ggcaagctgt tccagccgcg
    21901 cgacgccgcg gaggcgctgc tcgcgctggc gctgcgctgc gtgcgcgagg ccgtgcggct
    21961 cgacgccgtc gccatcaagg gcgtgcccga gaccttcaag ggcttccgcc acgggctgcg
    22021 ccaccggcgg ccggtgcgcc cggaggtctc gcgcttctac cgccacaact tcgagacctt
    22081 cgcgagcccg tggtggatgg cccgcccggt gcgggagctg gtgctcgagc tgccgcgcga
    22141 gctcgttcgc gagcgcacca cccgcaaccc gccgaagcac aggggccgcc gcgacgagta
    22201 ctacgagaag cgcgcgcacc tgtacccgct ggagcgcccc gaggcgctcc agctacgtca
    22261 gtcgtagcgg cggcgggtcg gccaccgccg tcacgcggcc cgacgccgcc tcgtgcagat
    22321 ggtccgccgt gagctcctcg ggccggcaca cgaccgcgag gccgcggcgc gcgagctccc
    22381 cggccacctg gagctggtgg tcgtccacgt gctcgccgtg ctccttgagc ctcggcacga
    22441 gcagcggccg gcgccccgcg tcgagcgcct cgagcgcggc gcccacgccc gagtggacga
    22501 ccaccgcgtc cgccgcgcgg aaggcggcgc gcagctcggc cgggggtacg gtcgtgtcct
    22561 cgcccgactg ccagagcagc acctccgcgt cctccggcag gaccgcgcgc aggcgctcga
    22621 ccaggcgcgg gaacgggaac agcgtgccga ccgacagcgc cacgcgcagc gggcggtcct
    22681 ccgccgccgc cgggacgtgc tcgtacgcgt cgaagaccga gccggcgtag cgccagcgcc
    22741 cggccgccca ggccgggtac tgcgtgtaga gccggacgcc cgggacgcgc gccagcgcgc
    22801 gcccggtcac cgacggcccg gcggcgcggg cggccgactc gacgtagtgc gactcgcggc
    22861 gcagcagcgc cgcctgcggc aggaacgaca gcgcgatgcc ggagcccgtg ctgaccaccc
    22921 gctcgtagcg gccgctgcgc agcagccgca gcgccagcgg gacgttgcgc gccaccgcgg
    22981 cgtagtcgcg cggccgggtg tagcgcgcgt gcaccacctc gcggcccgcc agcagctcgc
    23041 gcgactgccc ggtgtcgaag gtgacccacg tgggctcctc gcgcaggccg aggcgcggct
    23101 cgagccgtac gagctggctg aggtgccccc ccgtcgccgc gaccaggagc gtgctcacgc
    23161 cgacagcgcg gccagctcgt cgagccgctt gaaggggtgc acgaagtggc ggccggcgcg
    23221 cttcgccgcc agcgcctcct cgacgccgaa ctcgggcagc cagcgcacgt cggcgtagag
    23281 gtcgctcacc gcgtccatgt cgaccacgtg gaagccgagg tggtggacgg cggtcgggac
    23341 gaacatctcg acgtagtggg gcggtggctc gtcgagcgcg cggaaggcgg acagcgcgtc
    23401 gcgggtgagc agcaggcccg tgccgaggca gccccagcgc acgagcgggt cgtcgcgggt
    23461 cgtgatcgac tcgatgtagc ggtcgagccg ctcgtcgccg cggaccttga ggaagtgcga
    23521 ccagttcgtg tcgttgcgcg gcgtcgcgtt cttcgccacg agccccgcgc cggtgcgggc
    23581 gacgacgtca cgcagcgcgg cctcgaagtc gccgcgcagg atcacgtggt cgtactcgac
    23641 caggtagacc aggtcgacgt ccgggtcgtc gcggacccag cggtcgtgga tcgcgcgcag
    23701 ggtgtcgttg agcgacttgt cgaagtgggg cccgcgcagc gacgggtcct cgacgaagag
    23761 tgcgtcggcc gggtcgagcc cgtcgaagtc ggagcgcctg ccgccgtgga cggcgacgaa
    23821 gcgcccgtcc ggggcgagtg cgcgcatctg ggcgagctgt gcctcgacct gcgcgcggcc
    23881 gaggtgggtg aggaggacgg tgagcagcgc catgcggtca gcgcacccat ggacggtcgg
    23941 gcgacgggca ctggtcgagc tcgacgtggc gcagctcgag cgtgtccttg gccgtgaacg
    24001 cgatgcgcac gtgcgactcg cgcgcaccga cgatccgcag ccgcccgcgc cacacgaacg
    24061 ggatcccgcg gaacagcacc cggtcgcact cggcacagcg cagcggccgg ccgagcgcgc
    24121 gcagcagggc cgcctcggcg ccgcggccga tgcgcgagcg ggccacgcgg gcgcgcagag
    24181 ccctcacgcg cgcggctccg ccgagccgag ctcgcgcacc gcgtcgccga gttcgcgcac
    24241 cgcggccgcc gccgcgcggc ggcgcgagag cctccccgcc aggagctgcg ccacgcccgc
    24301 cgcgcgctgc gggtgggcga gctcgcgcgc gagcccggcc agggtcctcc agcgcggagc
    24361 gccggccgcc agcgcgcctc gcgcggtgtc ccaggccagc acgccctcgt ggcggcggcg
    24421 cagcgcgggg gtgcaccagg gctcccgggc gtgctcgtcg agcaccgact tgtgcgactc
    24481 gtgcatgcgc cgcgggtcgg cggagacctg gtcgtcgtgc acgtggtaga gcgcggtcac
    24541 gcgcgggatc gcgagcgccg atccgagctg gagcagccgc agccacatgt cgaggtcgga
    24601 cgcccgcttc agcgggcgaa agccgccggc gcggagcgcg gcctcgcggc ggaccagcac
    24661 ggagctggcg gtgagcttcg agtcgggcac cgccacgtcg gccggcccgc gcagcacgcg
    24721 cggccgccgc ccggtccagc cgaagacccg gtggtggttc gccccgctgc ccattccgag
    24781 gatcgccgtg cccaccagca cgtgcccgtc gtgcgcggcc cagagcgtct cgaggtgctc
    24841 cggcagccac tcgtcgtcgg agtcgagcag cgccacccac tcgtgccggg cggcctcgat
    24901 gccgctgttg cgcgccgcgc cctcgccctg gttgcgctcg tgcgtgacca cgcgcgcccc
    24961 caggtccgcg gcgcgcgagc ccgtgccgtc gccggaggcg tcgtccacca cgatcagctc
    25021 cgcgggcgcg gcggtctgcg cctgcacgct gcggatcgcg cgctccacga ggtccggccg
    25081 gcggtaggcc gggatcacga ccgagacggg cagcgggtgc tcctccatga gaccgcgtga
    25141 gcctattcgt gcacgccgcc gccatgcgga tccgctcatt tatcctggcg ccgacagtga
    25201 gagcgccatg gggcaggccc ccgaacgtcg agaagcttgc tcgcaaggag aacgtcaagg
    25261 gcctcgtcag ggcgctgcgc tacgaggatc cgatcagcga cacgggcggg cggacggccg
    25321 acctcggcgt ggaggtgcgc gccgcggcgg cggccgcgct agggcacctc gacacgccgg
    25381 ccgcgaacgc ggggctgatg agcgcgctcg aggaccccga ggagcgcgtg cgggtcgcgg
    25441 cggtgcgcgc gctgcgcgag cgcggcggca cgaccgcggt ggcgccgctc atgtcggcgg
    25501 cggcgagctg gaccgacccg gcgcgagagg aggcgcgcga ggaggcggtg gaggcgctcg
    25561 cctccgcgca ccacccggac gtgctgcgcg agatggtggc ggcgctgctc gcgcgaccgg
    25621 aggacctcga ggagccggac gcggacgtgg tccgccgcct cgccgaggcc acgggcgcgc
    25681 ccgccgcgct gcgcgacacg atcgaggacc tgatcgcaca cctcgtggag cccgccacgg
    25741 ctccgcgggc acgcacgctg ctcgtgtggc tcgcgcccga gagcgtcgag ccgctgatcg
    25801 cggccgcggg cgacgagaca gcggcgccgg aggccgcacg cgccctcggc gcgattcgcg
    25861 actcgcgcgc cgtggtcccg ctcagctcgc tcgtcgtttc gagtgaggat cccctggtgc
    25921 gggcggccgc tgcctgggcg ctcggggaga tcagggatcc ctcggccgtg ggcgcgctgc
    25981 tcgccgcgac cagcgacggc gactacgcgg tgcgcgccga ggcgctggag ggcttcgaca
    26041 agctcggcaa cgcggcgatc gcggtcgcga tgagcatgct cgtgcagccg atgctcgaga
    26101 acggcgcccg gcgggccgag gaggtcgagg gcgatcgcac cgaggagctg gccgccgccg
    26161 ctcccggtga gctgcccgcg gaggcgtccg gcgcaggggc ggccgccgac gcatccggcg
    26221 cggggccgac cgagcccgag ccgagcgcgc cggtgcaaga gcccggcgtc gcggtcgcgg
    26281 ctcccgccgc gccgagcccc gcgcagcagc cgctcgccac acgcgcggcg ccctacatca
    26341 ggcgcctgct gggccgctag gtgcagccgc gcagcacgtc ctcggggcgc ggcagcgggc
    26401 gctcctgccc ctccgtcccg acgagctgca tccgcgtgag ctggaggtgg cgccagggga
    26461 agctggcgag cagctcccac ggcggtctcc cctggaagag atcggcgtag gggtcggccc
    26521 ggaacgtcga cgcgttctgc gcgtagaacg ccaccgcgcc ggcctgctcg cgcaccacga
    26581 tcccgtagcg ctgcgccgca cgcgcgatcg tggcgaccgc cggcgggagc cccagggcgt
    26641 cgacgtcgag cgccgggtcg agccggaagc gcgcgccctc cggaaccgcg tggcggcagg
    26701 tcgagacgcc gtcggtgcgc cgcgcgggga ccgcgaactc ccaagcccgc acgtcgggca
    26761 cgccgagcgc gagcgcgtgg tcgatccgcc ctgagcgcag ctcgcgcggc gtgatcagcc
    26821 cgcccgccag cggcagcccg ctggcgctcg tgccccagtt cgagccgaac acggccgggc
    26881 cggtcgagac cccggcgagc cggccgcccc accgcgcgtg ccagcgaccg ctcttgcggc
    26941 gcagctgcca gaactcccag agcgtgtcgg tggagggctg ccacaccacg atctccgcgt
    27001 cggtgcccgg ggccgcgcgc gcgtcggcgg gcagggggac ggaggccagc gcggcgcgaa
    27061 ggtcgtccgc gatgccggcc ggctggtcga gctcgacctt cacgcgcggc tggtccgccg
    27121 gcaccgtgta gaccggcggg gtgtaggcgt ccgtgttgat agtcgcgggc cggccctgac
    27181 cggggcccgc ctcgaccgac cggatcaact cgccggtcac ctcggctgag gacgggtcga
    27241 gcggcgcgtc cgccggcagg gcctcgttcc agaacgcccg cggcgagaag aagcgctcgc
    27301 agtccggcgg gtcgagccgc agcggcgcgc tcgcgcgcct ggccttcgag tagcccttgc
    27361 cctcgaccgt caccagcagc ttgccggccg gacagctctc gagcagggcg cgcccggcgt
    27421 cgcccagcgg cagcttcacg gtgcgctggc gcccgcgggc gagcgtgacg acgcgcgcgg
    27481 cgcccagcgc ctgtgtgctg ccgtcggccc cgcgcagcgc cgcggccagc cgcacgcgcc
    27541 cgccggagcg gtggcgcagc tcgaccggaa cggccccgcg cgccagcagg tcggactggc
    27601 cggcggccgg gagcgaggcg tccacgggca ccgtgcgcgg ggcgcgcgcg gacgagatcg
    27661 cggccaccgt gagagcggcg gcgacgagag cgacaactac gaacgcacgg ttcggccccc
    27721 ccatgggcgc aatctagctc aggcgcgggc cagccatgcg ctaccgcggc ggatgcgcgc
    27781 cgtggccgcc ggcagggcca tcacgcgata cagcgagtag ccgatcgcgc aggcaccgcc
    27841 ggcgttcagg acgaacgcgt cgagcccgcg cgcgtcgacc acggcgaacc agatcatctg
    27901 cacgaggaag atgtgccagg acgcacggcc gatcgcggcg agcacgcgga ccggagcggc
    27961 ggtctcgcgg ctcggcagga atcgcagccc gagcagcacc agggcggccg cgtacggcgc
    28021 cgcgaggaag ttggtggtgg cgccgaagtc gtggcggagc cagtcgaacc cgccgtccga
    28081 ctggtgctcg aagaccaggt acgcgacgct tgcgagcgcg agcccgagga tccagcgatt
    28141 gcggcggtcg gcgagaccgg agtgctccgc gatccagagc ccgagcgcga tctggccgaa
    28201 gtagcgcagg atcgcggcgt cgtagacgaa ggggtagctc ccgctgaaca cgtgcggcgc
    28261 gatcagctcg aacgccgcgt cgatcgcgaa gcaggctgcg atcgtgacga gcggcgcgcg
    28321 ccggaaggcc aggaacagca gcgggaagac gagcacgaac tcgaacgcga tcgtcacgaa
    28381 gtagttcccc gggcccgtca ggggagtgac gccgaccgcg gtggtcggac cgaagccgac
    28441 gtcctcgctg agcgcgagcc cgagcacgag gctcgccacc cacacgacgg cgaagggcgc
    28501 cagcagccgc tcgaaccggc ccgcgaagta cgagcgggtg aagaggtcgc gcagtggagc
    28561 gtcgccgcgg cggcgcagcg acgacgcggc gttcatcgcc atcagcacga ggaagatcgg
    28621 gacggcctgg ccgaagtgga ggccctccca ggcgtcgtcc aactcgtgcg cggtcagtcc
    28681 gtgcagcgcg atgacgccga gcacggcggc ccccttcagg aggtcgatct gcgcgagtcg
    28741 cgcgggctgc gacatgcgtc caagtctcgc gaagcggctc acacgccgcc gtgcggaggc
    28801 cgacgcagac cgccccgacc atgccgcgcc ccctccgcac gctcgctctc tgcgtcgctt
    28861 tcgcgatgct cttcgcatgc tcgggagccg gtcccggagc gggtcccgtg cagggcgtgg
    28921 aggccgccgc ggcggcgaag cgggtcccga ccggagtcgc ggcgaagagg cgcgccgccg
    28981 cccgccgcgc ggccgcgaag aagaaggccg ccgcgaagag gaaggccgcc gcaaagcgca
    29041 agaagaagaa gccggtgaag cccccgcggc cgccggccgc gccgagcccc gtcgtgccgg
    29101 tcgcgccgag cccggccgtg cggaccccgg ccgtgcccac cccggcgctg ccggccgtgc
    29161 caagccccgc tccggccgcg ccggcccccg ttccgcccac cgcgcccgtg cccgccgctc
    29221 ccagcgcccc ggcgcccacg ctccccaccg ccaccgcccc ggcggcgccc gccccggggg
    29281 ccatcggcgc gctcgatgtc gtcaccccgt tcccgaccgg catgcgcctg ttcgcgtcct
    29341 cgagcttctg gaacgccccg ctcccggccg acgccccgct caaccccgcc tcgccgcgtc
    29401 tcgcgggagc cctcgcggcg gaggtgcagc gcgaggccgc ggcgcgcaag gggccgtgga
    29461 tcaacacgga tcagtggagc gtgccggtct acacggtcgg cgccgacgtc gcgcccgtgc
    29521 gcgtcgcgct cgacgtcaac aagccggcgc tccagcgggg cttcgaggcc gtcccgatcc
    29581 cgccgggcgc gcggcccgcg cgcggcttcg acggcacgct ggtgatctat cagcccgcca
    29641 cggacacgct gtgggagttc ttcgtggccc gccaggacgc gagcggctgg cacgcgcgct
    29701 ggggcggcaa gatgacgaac gtctcctcca acccgggcta cttcgacgcg ccctacggcg
    29761 ggtccgggac gagcctcgcg ctcctcggcg gcctgatgac gatcgaggag atgcgcgctc
    29821 ggaagatcga ccacgcgctc gccctcgcga tcccgaacac cgcggccggc gaggtcgtct
    29881 ggcccgccca gcgcggggac ggcacgacca ccggcacgga ggcgatcccg gaggggacgc
    29941 acttccgcat cgacccgcgg gtcgatctcg actcgctcgg cctgacgccg ctcggcctgt
    30001 cgatcgcgcg cgccgcgcag cgctacggga tggtcgtgcg cgacacggcc ggcaacgtcg
    30061 ccctctacgg cgaggacccg gtgaccacga ccggcgaccc gtacggcgag atctacgccg
    30121 ggcgctaccc gagcgatcac ctgcgcaact tcccctggga ccggctccag gtggtcgcgc
    30181 cgcgcggcgg ctgaccgatc agcgcgactg gagccccgcg gccgcgagcg gcgcccgtga
    30241 gcctccgttg cgcgagagga accgcgacag cgccggcgct ccgaccggcg tcccgcacgc
    30301 gacgccggcc cacgcccaca cgaggtaggt cgtgaacggg tagtgcgccg ggctgaacag
    30361 ccccaccacg agccacgcga cgagcaacgc cttcagcccg accttccagg gcacgagcag
    30421 caacgaatcg gaggggctca gcgcacggct gaacgagaac agcagcgcga tgagccacag
    30481 cccgaagccg atcagcccga gtccggccac gtagtacagg tagatgttgt gcaggccggc
    30541 gcgcccgccg gtcagcggga tggtctcgtc catccggaag tacggctcga gctctgcgtt
    30601 cgggcggtcc cagccgacgc cgacgaacgg gttgtgcgcg acgagccgca ggcccgcggc
    30661 attcgtgttc tcgcggtcgt agacgggcgt cttgtcgttc tggcgcgcgt tgacgtcgcc
    30721 cgccagcccc gggatcgcgg cgaacgcgac cagcaccccc acc
//
LOCUS       DASVPL010000294         4000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_309008, whole genome shotgun sequence.
ACCESSION   DASVPL010000294 DASVPL010000000
VERSION     DASVPL010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309008"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VF517_16520"
     CDS             <1..1055
                     /locus_tag="VF517_16520"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8054593.1"
                     /translation="RERAVADFVAGELRAAGLHVTEDASASETGSDCGNLLARVAGPP
                     GSRTILLCAHLDTVPLNPKDGFVEVVESEGVLSNRHESILGADNKSAVAPLLGVARRY
                     AAEPPPVGIELLFTTAEEPGLIGAKAFDRDSLQAEFGYVFDHASPWGELITAAPTYYT
                     IDAQFRGQSAHAGIRPEAGASAIAAAARAIARLELGRLDAKTTANAGTIEGGSARNVV
                     PERCDVRLEARSLDHDRANGAVHDMVDVLTEGASDGSCDLELTVEEVCRAYRIAQTAP
                     VVEIASAALRGLGIEAECIPTGGGSDASVFQARGLPCLNVANGTQANHQPDERVTIDA
                     LELGLDVALGIVSHAA"
     gene            1052..2089
                     /locus_tag="VF517_16525"
     CDS             1052..2089
                     /locus_tag="VF517_16525"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX8054594.1"
                     /translation="MSALALRRGTVSSVETAGPATRLTVDVDGEQRPAMSYERLTGPV
                     EQGDDVVVNVAARDLGLGSGGFDIVHVNLTRGLSGTGIEGAHVMKLNYTSMQHAVHPV
                     EEQAGPAGQQPSLDLPLRKPVAVISLHGQLPCVAWAAAQARPGLKVGFVQTAGGALPG
                     ALSDVVRELVERGLLCGQVTASPAFDGEHEAISAPGAIHAGLTTLGWDACIAGPGPGI
                     LGSATALGHGGLAGLDTAHAAMALGCSVTLVPRMSSGDARPRHAGLSHHTATVLELLL
                     KPALVAIPAGAIVSLPGEHEARAAEVDLDGYANSGLPARTMGRSIEEDELFFRSALAG
                     GVVLALESDGL"
     gene            2079..2615
                     /locus_tag="VF517_16530"
     CDS             2079..2615
                     /locus_tag="VF517_16530"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8054595.1"
                     /translation="MAFRRTGGEEAWSGKILAVRTDRFVHDDDGEEVEREIVVHPGAV
                     AMVAHDGESLYLVRQPREATGEQALLEVPAGKLDAEGEQPLDTAKRELIEEIGKGARE
                     WKHLVTAWSSPGFTNEQYHVYLATDLYDAEPDEDPEENERIEIVRWPLDDLDGLIEQT
                     TDAKSLIGLLWLRAYRAG"
     gene            complement(2804..>4000)
                     /locus_tag="VF517_16535"
     CDS             complement(2804..>4000)
                     /locus_tag="VF517_16535"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8054596.1"
                     /translation="VTRLENVFVPEENLIGKEGMGLKIALSTLNTGRLALPAICVGNA
                     KWATKVSREWSTERVQWGQPVGKHDAVAQKIAYIAASAFGLEAVVDVSGRLADDKKND
                     IRIEAAIAKLYGSEMGWKAIDELVQVRGGRGYETAESLRARGEKPIPVEQVLRDMRIN
                     RIFEGSTEIMHLLIAREAVDKHLEVAGEILEGDGDLKEKAKVAVQAGKFYAKWLPQLV
                     VGEGQKPGSYDELGSLATHIRFVEANSRKLARTTFYAMTRYQAGLERKQAVLGRIVDI
                     GAELFAIASAVVYADTIKREQPERGESAYELADLFAQQARRRVDDLFDAVWRNEDDAN
                     YRTAQRVLEGRFEWLEEGILEQSADKPQVAGQPDPAEAEADSSREATAEHANGNGATP
                     VPTPVQ"
BASE COUNT          544 a         1481 c         1410 g          565 t
ORIGIN      
        1 tccgcgagcg cgccgtcgcc gacttcgtgg cgggcgagct gcgcgccgcg gggctccacg
       61 tcaccgagga cgccagcgcc tccgagaccg gctccgactg cggcaacctg ctcgcgcgcg
      121 tggccggccc gcccggctcc cgaacgatcc tcctctgtgc gcacctcgac accgtcccgc
      181 tcaacccgaa ggacggcttc gtggaggtgg tcgagtccga gggcgtcctc agcaaccgcc
      241 acgagtcgat actcggcgcc gacaacaaga gcgcggtggc gccgctcctg ggcgtcgcgc
      301 ggcgctacgc cgccgagccg ccgccggtcg gcatcgagct gctcttcacc acggcggagg
      361 agccgggcct gatcggggcc aaggcgttcg accgcgactc gctccaggcc gagttcggct
      421 atgtgttcga ccacgcgtcg ccctggggtg agctgatcac cgccgcgccc acctactaca
      481 cgatcgacgc gcaattccgc ggccagtccg cgcacgccgg catccgcccg gaggccggcg
      541 ccagcgccat cgcggcggcg gcgcgcgcga tcgcgcggct cgagctcggg cgtctcgacg
      601 ccaagaccac cgcgaacgcc ggcacgatcg agggcggcag cgcccgcaac gtggtgcccg
      661 agcgctgcga cgtgcggctc gaggcgcgca gcctcgacca cgaccgcgcg aacggtgccg
      721 tgcacgacat ggtcgacgtg ctcaccgagg gtgcgagcga cgggagctgc gacctcgagc
      781 tgacggtcga ggaggtctgc cgcgcctatc ggatcgcgca gaccgctccg gtggtcgaga
      841 tcgcgtcggc tgcgctgcgc gggctcggca tcgaggcgga gtgcatcccg accggcgggg
      901 gcagcgacgc gagcgtcttc caggcccgcg ggctgccgtg cctgaacgtc gccaacggca
      961 cccaggcgaa ccaccagccg gacgagcgcg tgaccatcga cgcgctcgag ctcggcctcg
     1021 atgtcgcgct gggcatagtc tcccacgccg catgagcgcc ctggccctcc gccgcgggac
     1081 ggtctcctcc gtcgagaccg ccggcccggc aacccgcctg accgtcgacg tggacggcga
     1141 gcagcgaccc gccatgtcgt acgagcgcct caccggaccg gtcgagcagg gcgacgacgt
     1201 ggtggtgaac gtcgccgcgc gcgacctcgg cctgggcagc ggcggcttcg acatcgtcca
     1261 cgtgaacctc acgcgcggcc tctccggcac gggcatcgag ggcgcgcacg tgatgaagct
     1321 gaactacacg tcgatgcagc acgccgtcca tccggtcgag gagcaggccg ggcccgccgg
     1381 ccagcagccg tcgctcgacc tgccgctgcg caagccggtg gcggtgatct cgctgcacgg
     1441 tcagctcccg tgcgtcgcct gggccgccgc gcaggcccgg ccggggctga aggtcggctt
     1501 cgtccagacc gcgggcggcg cgctgccggg cgccctctcc gacgtcgtgc gcgagctggt
     1561 cgagcgcggc ctgctgtgcg ggcaggtcac ggcctcgccc gcgttcgacg gcgagcacga
     1621 ggcgatcagc gcgccgggcg ccatccacgc cggtctcacc acgctcggct gggacgcctg
     1681 catcgcgggc cccgggccgg ggatcctcgg ctcggccacg gcgctcggcc acggcggcct
     1741 cgccgggctc gacaccgccc acgccgcgat ggcgctcggc tgctcggtca ccctcgtgcc
     1801 gcgcatgtcc tccggcgacg cccgcccgcg ccacgcgggc ctctcgcacc acacggcgac
     1861 ggtgctggag ctgctgctca agcccgcgct ggtcgcgatc cccgcgggcg cgatcgtgag
     1921 cctgcccggc gagcacgagg cgcgcgccgc cgaggtcgac ctcgacggtt acgcgaactc
     1981 gggcctgccc gcccggacga tgggccgctc gatcgaggag gacgagctgt tcttccgctc
     2041 cgccctggcc ggcggggtgg tgctggcgct ggagagcgat ggcctttagg cgcaccggcg
     2101 gggaggaggc ctggagcggc aagatcctgg cggtccgcac cgaccgcttc gtgcacgacg
     2161 acgacggcga ggaggtcgag cgcgagatcg tggtccaccc cggcgcggtc gcgatggtcg
     2221 cacacgacgg ggagagcctc tacctggtgc gccagccgcg cgaggccacc ggcgagcagg
     2281 cgctgctcga ggtcccggcg ggcaagctcg acgccgaggg cgagcagccg ctcgacaccg
     2341 ccaagcgcga gctgatcgag gagatcggca agggcgcgcg cgagtggaag cacctcgtca
     2401 ccgcctggtc ctcaccgggc ttcacgaacg agcagtacca cgtctacctg gcgacggacc
     2461 tctacgacgc ggagcccgac gaggaccccg aggagaacga gcgcatcgag atcgtccggt
     2521 ggccgctgga cgatctcgac gggctcatcg agcagaccac cgacgcgaag tcgctgatcg
     2581 gcctgctctg gttgcgcgcg taccgggcgg gctgaggcga cctagccctg ctcgtcccac
     2641 gcgggagcac ccgcccggcc acacggttct caagccctcc cggccgtcgc gcatcgattg
     2701 gagggtttct ccgctatgcg ggacacaagg ctccaatcga tgccgcaact ggtcgacctt
     2761 gaccaccgca tgggtggctt aggtcgacca gttgggctga ccgctactga acgggcgtcg
     2821 gcaccggcgt cgcgccgttg ccgtttgcgt gctcggccgt ggcctcgcgt gaggagtcgg
     2881 cctcggcctc cgcgggatcc ggctggccgg ccacctgcgg cttgtcggcg gactgctcga
     2941 ggatgccctc ctcgagccac tcgaagcggc cttcgagcac gcgctgcgcc gtcctgtagt
     3001 tggcgtcgtc ctcgttgcgc cagaccgcgt cgaagagatc gtcgacgcgg cgccgcgcct
     3061 gctgggcgaa caggtcggcc agctcgtagg cggactcccc ccgctcgggc tgctcgcgct
     3121 tgatggtgtc ggcgtagacg acggccgacg cgatcgcgaa cagctcggcg ccgatgtcca
     3181 cgatccgccc gagcacggcc tgcttgcgct cgagccccgc ctggtagcgg gtcatcgcgt
     3241 agaacgtggt ccgggccagc ttgcgcgagt tcgcctccac gaagcggatg tgcgtggcga
     3301 gcgatccgag ctcgtcgtag gagccgggct tctgcccctc accgaccacg agctgcggaa
     3361 gccacttcgc gtagaacttg ccggcctgca cggcgacctt ggccttctcc ttcaggtcac
     3421 cgtcgccctc gaggatctcg cccgccacct cgaggtgctt gtccacggcc tcgcgcgcga
     3481 tcagcaggtg catgatctcg gtcgagccct cgaagatgcg gttgatccgc atgtcgcgca
     3541 ggacctgctc gacggggatc ggcttctcgc ctcgggctcg cagcgactcc gcggtctcgt
     3601 agccgcggcc gccgcgcacc tgcacgagct cgtcgatggc cttccagccc atctcggagc
     3661 cgtagagctt cgcgatggcg gcctcgatcc ggatgtcgtt cttcttgtcg tccgcaaggc
     3721 ggccggagac gtccacgacg gcctcgaggc cgaacgcgct ggccgcgatg tacgcgatct
     3781 tctgcgccac ggcgtcgtgc ttgccgacgg gctggcccca ctgcacgcgc tccgtggacc
     3841 actcgcgcga gaccttggtc gcccacttgg cgttgccgac gcagatcgcc ggcagcgcca
     3901 ggcgacccgt gttgagcgtg gagagcgcga tcttgaggcc catgccctcc ttgccgatca
     3961 ggttctcctc cggcacgaag acgttctcga gccgggtgac
//
LOCUS       DASVPL010000295        29737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_114062, whole genome shotgun sequence.
ACCESSION   DASVPL010000295 DASVPL010000000
VERSION     DASVPL010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 29737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29737
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114062"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..519
                     /locus_tag="VF517_16540"
     CDS             184..519
                     /locus_tag="VF517_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054597.1"
                     /translation="MTRTRLFAERFLVEVGDEGPLPPSGEYDEARSITVAEDGTPAVE
                     GDTEQTKTYDQRAAAFYDTDDPSSCAPWASTTTQTFVREEEPADRDHHITAWAGTVTD
                     IKGPAIDAD"
     gene            521..1480
                     /locus_tag="VF517_16545"
     CDS             521..1480
                     /locus_tag="VF517_16545"
                     /inference="COORDINATES: protein motif:HMM:NF044697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054598.1"
                     /translation="MVLVVTNRRDLTADWLILELERRGAGYVRFNTEDYPASTSLTWE
                     LDGPTTLSIGGAEHRLEPPLAIWYRRPLPPRLGDGLPGDAAEWAAGEAEAALTGVWRT
                     RDAVWVNHPDANDRAGSKPEQLRTAARLGLDVPRSVVTDDPEAVRRLAAEVGGELVCK
                     PLRSGHLSIDGEEGLFFTSRLTSLDGELDALGPEPYLFQELVPKLYDVRVTVIGEETF
                     ATRIESQSEQDARIDWRRVDAGRLEHRVEALPPEIAERCVALVRHYGLRFGAIDFARE
                     PSGRYRFFELNPNGQWAWVEQFTGAPMRSCLADLLLQGDAALA"
     gene            1464..2198
                     /locus_tag="VF517_16550"
     CDS             1464..2198
                     /locus_tag="VF517_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054599.1"
                     /translation="MPHWRDDIAELAALLGFSDRRPPPPEPDVFEEKTEELAAKLAVK
                     PELADALLEEAKDHHEYANERAESAERRATTLQGAVAIASSLTLAGGSLLLDSSKLQG
                     IGWKIALGLGLVGVTLCLIMAGYRAVTATSRLYQWAAPDPVDIFVHAGLDSPEAVKVA
                     RAADLLKASGINQAVASRKVVAMGEAAMWFTRALFLILALAVLLAVYGAVGRHEPAKK
                     DEAAQGTKGDRGAGVRSARVARGSRS"
     gene            2472..2545
                     /locus_tag="VF517_16555"
     tRNA            2472..2545
                     /locus_tag="VF517_16555"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2506..2508,aa:Arg,seq:acg)
     gene            2555..9235
                     /locus_tag="VF517_16560"
     CDS             2555..9235
                     /locus_tag="VF517_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8054600.1"
                     /translation="MPDRGACIRRHARIAWRGADPVERSEYISHSARLWQSREAGRVT
                     RISWLHLSDWHQTVNGFGRSVVRDALLDDLADRTALDENLDEIDFVVFSGDLASKGHA
                     DELSAARGEFLDPVLARVEVDRQRLFLVPGNHDVDRDAFELLPKALLAPFSDESAVEK
                     WLTDDERRDLVLRPTRAYSELAAEFGFDGNPAYAAHGHLNVRGVEVGLVGLNSALMAG
                     RDPGPRTPGRDEDYGLLVVGEPQAREALRHTEGADVQIAVVHHPIDWLQSFDRNRLEP
                     ALASTAHFLLHGHRHLAHCDKVRGTSGDHVVIPAGASFTERSASDQRFANAYTLVTLD
                     LERGTGVAYLRRWGDRRNKWIADEDTTPGGASSFVLPAALCVSPPTSPGRGESSGRRQ
                     QGQDDRTDLAHTRYLRAVVKDNAELEPRGVSQAREGQALPLEEIYLQLSAVADKPDVD
                     RRVMQEEFEEVGRQLDLIEDPAEREREFERWAQQATLLHKTLDMTVPSQRLHDIAREQ
                     RAAVILGDPGSGKTTLLRYVALTFAQAILANPGAVLDPTDPWDPGSATQAGDLGTVRL
                     PILVRVSEFAEARRGNPQLSLVDFLTDYFTGLQIPDAHELAPLFHTLLDAGRCIVLLD
                     GLDEIPDPAERIAVAMAVRRFIAAYSEKGIPVDASSPRAGPQHLRSLLTDARVIHTGN
                     HFLVTSRIAGYHIAPVGGVELFTIRDLEQPQIEEFLGRWCHAVARHAPLGRETGAAEE
                     RARREVAGVLRAIAESPGVRRLASNPLLLRILATVHQTKGHLPQRRVQLYETAALTLL
                     QDWNLAKGDAGTAIDEDEAVRLLGPLAFHMHEAHVTPFVTLAEARRLLARERARQSGE
                     EPEKPSARTEREVNELLERVRQHAGLFLERGEGLYGFMHLTFQEYFAARELTSSARTA
                     KRLILKRLHEPRWREPILLAVGILSRQFYDDTREVLLAIRDAPAEHEDLLHRNLLFVA
                     HCIGDCVAAPDALVRDVAGRLLELWSDRTGSGRYKLLQRQVRAALELLAGEGRGAVDE
                     ALAESLRRCAANGGACPPVDLVEPLSARGAPVAPTLQAAVGQADACVHELIDRLVALR
                     FAQSPSTRIHDAMLREPAFARLVGLLRVSGWEHRIALLLRAAGDFEDENASELAFLMT
                     AADSVDEIRMAVALARSSARASFANVFLARMYKSAPPDAELKAALDDVSNALTLPGRD
                     RAEVARVDDAVGGLEEAARRVREEVLRRPAADTVERLGRPMLDAARPVSAVVPHLAAV
                     GALLLGSGEAPPPSALDAKHAIVARLHKALAATGDPLEYHDITAFLLSLQDEGREGVV
                     DASAVAASLVRDATRSGARQRVALATMCSEEVQSHVQLTHDQRYALLSAEGPEGTAIA
                     RLQASVHGTLDGRSLRVCFDLLCRSDAALHETLLSRLGTARAQGSRAGLGVLEGALDD
                     AAVRPVALDLMKRIEWVDGETLAWTLAAVESDDFEMRAHAAVLLARQEHVYHEVRLAL
                     LQPPASPGVTGDGDSWRQLANETTVVRLLALLMTNGWHDALSYALTGHAAGHKDVIAR
                     GHEFKRALRIERDAEERIRRALRYDQHVLTRRHHATLVAALRGAAEAAGLRDTTGWQN
                     PEIVQRAESALVANIRRTPHLIGILPLGELRRLAQERTGRVADWVATAVASAGGGDAS
                     ASGLAALLRAPSAAVRRGAALSLVAEDLHAKVMPVLAEAMDIADDRLRRSARDAFMSL
                     GTQLSGYNESAAVDWLRSRDYDELPGYASGAFAAALGDVYCVRPQRLEQQLSDDRIKF
                     DSMSPALVPLLRRHLSEASGSAAEEGHSVLAAICTTNSAPYEPGFHRVPLDLMDSTDS
                     DARALGAYTLRWSADDDAAEAIARLLASAETSDDESLVAAALGSVGTLAARAPKGGDS
                     HTVETLRKFARRDAERVAAAARMAIGRIELARDVAPADMTVGTTEQVLAAALGGVTTA
                     QSGSKPRVVEVSKAVAGWVTGNEEASAQPSIDLLLDWLDESLRNRPDEGPTGGERAAG
                     IVQREFGWPFRCAVACTLAELSERLTWRSFTAKRDLVDVIDLLTDAGSDRMSWFVRQQ
                     AIRALGNLQLLTPEVVSVLFDACRDSSEVYAQARTAVTNFRRFSPQTLARLSRATADE
                     SVMVAEHTARLLGALGTSRSEELGAKGRKEIARELVKVLGSPHCERAVYNASGAKTGQ
                     LFDVVFEALVRVVDPSVMIHSRPAPGGFEGLPLGLR"
     gene            complement(9286..9960)
                     /locus_tag="VF517_16565"
     CDS             complement(9286..9960)
                     /locus_tag="VF517_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8054601.1"
                     /translation="MIAATRVKVDFKGLRATVAGRPVRDPARRLRVLVVDDQDLALWG
                     LRTLFIAESWVECCLTADNPGQALEVTRRYEPHVALVEMLLGTESGTNLCAKLREISP
                     MTHVLLTSATGRITGHSAQELGASGFVPKGWRVQDIASATRMVGLGMSVFAPEPKRMR
                     HVLSERELDVLQLIARGSTNREIADALFLSPHTIKDHASAIYRKMKAKNRADAIQRAQ
                     RLGLLV"
     gene            complement(9957..10190)
                     /locus_tag="VF517_16570"
     CDS             complement(9957..10190)
                     /locus_tag="VF517_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054602.1"
                     /translation="MRDLKAGGRRSFVRLLEVDPEFAVDLDEQESGLASKYLLARSAT
                     LPGGQWDPAREPPRQSARALGVLMMRTYRGRIA"
     gene            10588..13842
                     /locus_tag="VF517_16575"
     CDS             10588..13842
                     /locus_tag="VF517_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8054603.1"
                     /translation="MPDAMERAVGVHREIVSEAVARGGGVELAAEGEGSGAAATFRSP
                     AAALRAALDVQRAVRAREWPDGLAPRVRIALHAADLKHGLERNDAALALGRCSRLAAI
                     ACGGQTLVPRAIRELVVDRLPDGVELVDLGLHRLPDLGLPEHVYGVADGPQPEAPDAL
                     RSLDTLPNNLPYGLTSFVGRERELAEVAGVLGGARVLTLTGAGGCGKTRLALQVAADA
                     LDRFPDGVWRVELAPVSDTERVGQAVAEAVGVRPHRGQTALEAAAAHLKERRTLILLD
                     NCEHVLEAAAEAAETLLRGCPGATVLATSRAPLDLSAETTWRVPSLSLPEDGSGESPH
                     ALAASDAVRLFIERATKVRPEFTEATAPVLAKICSELDGIPLAIELAAARVRLLSVEQ
                     IATGLSDRFRILTGGTRTALPRHRTLRASVDWSHALLSDEDRTLLRRLGVFVGGFTLE
                     AAEQVCAVDERERCAIIDVLASLVDKSLVVVEERGRVARYRLLETVREYELELLREAD
                     ELEAIRDRHCDFFLALAERVAPALLTSSAHQELDALDEDRANLDAALGWATETDGERA
                     LRLCAALIGGGLQRRRFTARDAACARALEKAGPEPTRLRAQVLSGRVLLLFYAGRHEE
                     ATAVAQEALEVAEEVGDPAEIGRALEALGKNAIATSDPAAVRPLLKRACALLRASGDD
                     FSLVTTTQTMAWGHMYRGEYEEGEALLEEVFPIVERLGNPELLWWHWVGMALRHQHDG
                     ETERYFEFCERGLEAAREIGGPGESFIHSQIAIVELAQGRTAQALARLEASRERAVAA
                     GAGLAFLSTELLLASAEAVLGDLDGARARLEALAASTADGIQLGWMAAQLADFLRVGG
                     DAAGAEDWARKGLQVNERVAFPHCLSWCKEVLGRLAADRGDWSEAEAFFHEALALRAE
                     KQQRLWLAQGLDGLAVVAAGLEAHGEATRLLGASARARSDLGTVRWALDQPHFEALER
                     DLRVELGDEAFGAAWAEGSRLTIEEAVAWIRRARGSRKRPAAGWESLTPTELEVTRHA
                     AEGLTNAEIGKAMFVTGGTVKTHLSHIYAKLGLRNRAELTAEATRRRSGNSAEV"
     gene            13973..14431
                     /locus_tag="VF517_16580"
     CDS             13973..14431
                     /locus_tag="VF517_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054604.1"
                     /translation="MSYVAGEARQELLDTLGDAIDEIAAALAALGAAYELLSTAPADR
                     LEEELFRPVQAAYGRAKKTHAGFAQRQGIPPRTFTPATPGPPSTGIRGFVDSAVDSAA
                     DADAVLVELQDSMAPVEVGDPELRAGLSEVRELLGDVRARAHEFVRTLGR"
     gene            complement(14456..15283)
                     /locus_tag="VF517_16585"
     CDS             complement(14456..15283)
                     /locus_tag="VF517_16585"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX8054605.1"
                     /translation="MSNETFVNYWIGQEPTPPSPGLNEMPPEVDVAPLAFVTIDDQYA
                     LDFDFLCTTNSASVIQGWIKDVRANGTKVLFSINDTKLGTVPDVDAFVGGVAQQAVEW
                     GVDGVDLDYEPSYLEPSQTLLDVTKALRPALTDALGRDPLLTAPIWGLWDSQLEFLGQ
                     FAAELDFVTTMDYTPWPGVDETISNFDVYAGAIGSSEKIAIGLSCMGPPGSPPSEDGA
                     NFTPIDEVETICKWEPDGGTKKGVMLYTYSYDVESRKGSGTGLPDGTFTKTIIANLP"
     gene            complement(15377..16873)
                     /locus_tag="VF517_16590"
     CDS             complement(15377..16873)
                     /locus_tag="VF517_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8054606.1"
                     /translation="MTVSWEAHLPAGVRAEDVDLTAKGSLPAAWAEVWASAPSAPILH
                     DRERGWIDAHQLEDATRRVAGRLQAAGLEPGDRMLFSAESSLELVVAHVAALRSGIVV
                     VPTNTAYREREVTHVVSDSRPKAALVDDPDRAGWIRAAAGQETVVVGPEVDLPDREPA
                     VLDAADPGDPALLGYTSGTTGSPKGAVLTHSNLLAGSESVGLAWRWTAADRLVLALPL
                     FHAHGLCVGLHGTLLAGASAVLLPRFDVDAVLDAAGDQDATMFFGVPTMYHRLAQSPR
                     VGELARLRLCVSGSAPLPAELHGTLAERGGQQVLERYGMTETIMNVSNPYDGERRAGS
                     VGLPLPGVELRLAGGEDGEIQLRGPNVFGGYWERPEANAESFEDGWFRTGDLGGLDAD
                     GYLRILGRSKELIISGGFNVYPREVEDVLLGHPDVAEVAVVGTPSDEWGEVVTAFVVP
                     AGERPAADSLVDFAAERLAGFKRPRVVHFVDTLPRNALGKVLKHELAP"
     gene            complement(16920..18287)
                     /locus_tag="VF517_16595"
     CDS             complement(16920..18287)
                     /locus_tag="VF517_16595"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEX8054607.1"
                     /translation="MNGHHEMPTSGRALSSVALSATLHCLTGCAIGEILGMVIGTALG
                     FSNLGTILLAVALAFVFGYTLTSVPLLRAGLALGAVVPIALASDTASIAVMEVVDNAL
                     MVVIPGAMEAGLDELLFWASLSVALVIAGSFAFPLNRWLIARGKGHSAVHETGIHGGP
                     DVRVVGALAAVAFVFGSAVLIGDLVSGDDSGGGHGGGHSAMSGERGHEQEQDAVRGLA
                     VSEDGMKLELERAEMPRGTGSRLSYRILGSDGAAVRDFEVEHEKRMHLIVVRRDMTGF
                     QHLHPTMAADGTWSTPLTLDEAGSYRVFADFKRDGRNHTLASDLAVDGATAWEPIPAP
                     RAEASAGGGFEVMLASGASSAGEESELEFEVMHGGKPVEVDPYLGARGHLVALREGDL
                     AYLHVHPVEGGAGHEGGGHGGGSTGGPIAFATEFPTDGRYRLFLQFKVDGIVRTAAFT
                     RDVAR"
     gene            complement(18284..20512)
                     /locus_tag="VF517_16600"
     CDS             complement(18284..20512)
                     /locus_tag="VF517_16600"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX8054608.1"
                     /translation="MPETAEHLELPIAGMTCASCANRIERRLNKLDGVSASVNYATER
                     ATVDYDPAAVETTDLVAAVEAVGYQAVLPEDEPGADEPERDDTAPLRRRLIVTALLSA
                     PVLALSMIPALQFDRWQWLVLQLATPVVLWGAWPFHRAAWANLRHFAATMDTLVSVGV
                     LTAWTWSLYALFLGDAGETGMTMSLDLIPDARGGTHEIYLEVASVATTFILAGRYFEA
                     RAKRRAGAALEALLELGAKDAALLEADGSERRVPVEQLRPGSRFVVRPGEKVATDGVV
                     EEGRSAVDMSMLTGESVPVEVEPGAEVAGATVNAGGRIVVRATRVGADTALAQIARLV
                     TDAQSGTAPVQRLADRVAGVFVPGVILLAIGALGFWLGAGEGLGFAMTAAVAVLIVAC
                     PCALGLATPTALMVGTGRGAQLGLLIKGPEVLEATRRVDTIVLDKTGTVTTGRMTLVD
                     VALAEGVDRAEALRVAGAVEDASEHPIARAIADAARGELGELAPVRDFANVAGLGVEG
                     TVEDRAVIVGRPALLAERGLALPAALTTALRDAEARGRTAVVAGWAGAARAVLVVADS
                     VKPTSAGAIRRLRELGLRPILLTGDNETTARAIAAEVGIEDVIAEVLPSGKAEVVERL
                     RAEGHVVAMVGDGVNDAPALAAADLGLSIGTGTDVAIEASDLTLVSGDLRAAVDAIRL
                     SRRTLTVIKQNLGWAFGYNVAALPLAAAGLLNPLIAGAAMALSSVSVVLNALRLRRFG
                     SA"
     gene            complement(20512..20715)
                     /locus_tag="VF517_16605"
     CDS             complement(20512..20715)
                     /locus_tag="VF517_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX8054609.1"
                     /translation="MTSDTTTYSVPGVSCGHCSTAITAELTQVAGVEGVDVDLDAKTV
                     TVRGEVSRDAVVAAIDEAGYDVA"
     gene            20821..21123
                     /locus_tag="VF517_16610"
     CDS             20821..21123
                     /locus_tag="VF517_16610"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX8054610.1"
                     /translation="MATESSTPSRGYSATKDQLVKRLRRIEGQVRGIERMVEDDRYCI
                     DVVTQITAIQAALDKVSLGLLDQHARHCVVDGHGEGGPEELTDELMGAVARLMRRG"
     gene            complement(21145..23778)
                     /locus_tag="VF517_16615"
     CDS             complement(21145..23778)
                     /locus_tag="VF517_16615"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054611.1"
                     /translation="MLKRRGRIVIVGLMCAASAVGVTAVSGAPLPQSGTLDLLDGADV
                     VIDGLPAGSETGYAVGAAGDVNGDGRPDMLVGARRASAAGRERAGAVYVVFGRTDKAP
                     IDLANLGDGGFRIDGAAAGDEAGSAVASAGDVNGDGLADVVVGAPNADRATDATDSGA
                     AYVVFGKKGAGPVDLAALAGAGYRIAGAAAGDGAGFAVSSVADLNGDSVRELLVGAPR
                     VDVGGRADAGTAYVVFGSKVPADVDLASLGSGGYRLEGAQNGNAGWSVAGSPDMNGDG
                     RDEVLLGAPYTSPDESGFAGTAYAVWGKSSTATVDLANLGDQGYELHGGETGGPGDQP
                     STEAAGGAVAPVGDTNADGIPDFAVGGQLADRGALRNSGSVYIVYGRKEAGSLPLDAV
                     QGGNGWRIDGANAGDQTGATIASAGDFDGDGLGDIVIAAPLANPFSRQNAGASYVVFG
                     RAENSDVDLSALGPRGVRIAGAEPGDVTSRSVAGIGDMNGDGGPDVLLGSVYNRNGQP
                     PARDPATGEPGEPEAGAPEREPTGAAYVVYGPRPEPDAPPPDPGETEEVQEDKCVAAR
                     NVEAIIDDSGSMEATDPEVLRRLALEILLTKPRNQGETLGAVEFGSTADQLFPPLEVE
                     AGQEQDELLALIERELQADNGGTNYNAAFAVMADENPGAAARIFLTDGGHNEGTYALG
                     HLNGPPTYVIGLGIGRRGGDAGRLQRIASETGGRYFPSVDEGELQQVLNGIDSVLNCD
                     IDIATLDAIVTDDPEADEDEDELAEWDEELDEDAHSADVVVSWDDEDDDIDVDEMAVE
                     DDDGDVVQRIRAKSLRRLLKSGKRGGRHGLRVQGFAGDTYQSFRLTGLKPGTLKLRVR
                     AREVRGKTRVTAQISQSRRRR"
     gene            23902..24120
                     /locus_tag="VF517_16620"
     CDS             23902..24120
                     /locus_tag="VF517_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054612.1"
                     /translation="MDDDQMERFGEAVEQKKQESRERSEQTRDEPGSPGAVDGDQPDL
                     VEQGPQDVRDERKKNSGHGKVTADKWNQ"
     gene            complement(24145..24519)
                     /locus_tag="VF517_16625"
     CDS             complement(24145..24519)
                     /locus_tag="VF517_16625"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HEX8054613.1"
                     /translation="MAQQQHDVPGEVSHSLQMQISNEMVHMYKELFGRGPTKARTSYA
                     GPDAILCTLEDSLTPAERSLADLGEHQRLRDVRMFFQHAREADFRAAIERITGRQVRA
                     FVSGMDTEADVSAELFYLTPEA"
     gene            24669..25448
                     /locus_tag="VF517_16630"
     CDS             24669..25448
                     /locus_tag="VF517_16630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX8054614.1"
                     /translation="MAPRSIWNGTIAFGLVAVPIKLYSATESKTVHFHELHRRDEARI
                     EHRRFCSKEDKEVDNEEIVKGYEVSEGEYVVLSKDEVAGADPSRGKVIEIEHFVAADE
                     IDPVYYDRAYYVGAQDDGEAAYRVLHGALERAERVGIGRFVFHNKEQLVALRALDGLL
                     GLHTMRFDDEVVDGKDLDVPSPQKKPSKREVEMAGTLVSSLETKFKPEKHEDTYRDAV
                     MKVIDRKASGKEVELPDRHHPELAPDLLMAALEASVSKGKG"
     gene            25450..26247
                     /locus_tag="VF517_16635"
     CDS             25450..26247
                     /locus_tag="VF517_16635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX8054615.1"
                     /translation="MARSIWNGSLSFGLVNVPVALFSAVRDEGVHFRQLHEKDGAPID
                     TRRYCSDEDVEVPWEEIVHGYEVSKGKWVTLSDEELASAEPRKTRTIDIEEFVDLAEV
                     DPIYFDHPYFLVPPDSEGAARAYRLLVEVMEGSDKAAIGRFVLRTKEYLVALRVRDGA
                     LTLTTMLFHDEVRPTKGIAPSKAKKTAPAKEELDQAVALIEALTTEFDPSSYRDRHRE
                     RLLKIIRDKKKGRKIKLPEPDGEPEAIPDLMAALEESLAKAKSKAKA"
     gene            26304..26915
                     /locus_tag="VF517_16640"
     CDS             26304..26915
                     /locus_tag="VF517_16640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054616.1"
                     /translation="MAGLNGGDPAAAGRIRAAAMEAFAAYGDAVSIRAIADAAGVSPG
                     QVQHHFPTKAELREAVNEHVLSIAAGAFAGLDGENGDVFENLAQRVTSIMREHPDALL
                     YVARAAVDGDPGGLGIFDGFMAVATTQLEGLAADGRLDPDLDLGWAALHVVIFNLATV
                     LFEHAIESHLPEPLRSPEGVARWHAADTELFRRGFLRAKAARG"
     gene            27088..29232
                     /locus_tag="VF517_16645"
     CDS             27088..29232
                     /locus_tag="VF517_16645"
                     /inference="COORDINATES: protein motif:HMM:NF023853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEX8054617.1"
                     /translation="MPKIRQHNVTAARWSANGGSLGAVDLTKDASYPFGQNVFSIEVQ
                     RERLPQDVCDRLQGTLEKGAALDPSLADEVARGMKEWALERGATHYTHVFQPLTGLTA
                     EKHDSFFSPTGDGSALAKFSGKELIQGEPDASSFPTGGVRATFEARGYTAWDPTSPAF
                     ILENPNGALLCIPTAFASWTGEALDAKIPLLRSMDALSRSAIRALRLLGDDQAERVFT
                     TVGPEQEYFLVDEQYFFERPDLVTTGRTLFGAQPAKGHELDDHYFGAIPERVLAFMMD
                     SELELAKLGVPVKTRHNEVAPGQYEIAPIFENSNVGSDHQQLTMQVLQNVARKYGLVC
                     LLHEKPFAGVNGSGKHNNWSMGTDTGHNLMEPGDTPAENLNFLFFCAAVVKAVDKHQG
                     LLRASVANIGQDHRLGANEAPPAIISIFLGGELERIFDAITSGEGDPNTPASFLNLGT
                     DVLPPLPKHGGDRNRTSPFAFTGNKFEFRALGSSMSLAFPNTVLNTIVAEAIDELADQ
                     LEPKLSGGDLGAAVTEVVKDAYSGSKRIIFGGDNYADEWHAEAESRGLLNLRTTPEAL
                     PEVISEQTIGTFEKYDVLSHRELDSRFEVWVEQYTIRANIEAETAATIARTMLLPAAL
                     RHIALIEAAGIDTLAAETRALTERFANSIADLEAANAYPDGVEGIQLAEYARDSQLTA
                     LAAVREIADLLEKVVADDLWPLPKYSEILFIK"
     gene            complement(29272..29592)
                     /locus_tag="VF517_16650"
     CDS             complement(29272..29592)
                     /locus_tag="VF517_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054618.1"
                     /translation="MTPRLLAILCALMLSIAAGCGEDGDGSDSPATLADAKKALVDDC
                     HKGHEGDDKDLALCRCIADQLEKKHGYDTPEKFEKTRKRVADEDVPPEVQAAAAAPEC
                     QKTQ"
BASE COUNT         4314 a        10574 c        10659 g         4190 t
ORIGIN      
        1 tcgcgggccg atcctaacct ggcacctgga cgctcgataa cccccctgtt tggggggtcg
       61 gtgagacggc ctcccccgcc gactcgcgcg tcccccgtcc ggcacccccc cgggcgctgc
      121 cgggagcgta cgatgcgtcg aacgcgccac ctgggcgcga cgtcggacca ggaggactga
      181 acgatgaccc gcacccggct cttcgcagag cgcttcctcg tcgaagtggg ggacgagggg
      241 ccgctcccgc cgagcggcga gtacgacgag gcgcggtcga tcaccgtcgc ggaggacggc
      301 acgcccgcgg tggagggcga caccgagcag accaagacct atgaccagcg ggccgcggcc
      361 ttctacgaca ccgacgaccc ctcgtcgtgc gcgccgtggg catccacgac gacgcagacg
      421 ttcgtgcgcg aggaggagcc ggccgaccgc gaccaccaca tcacggcgtg ggccggcaca
      481 gtgaccgaca tcaaggggcc ggcgatcgac gccgactagc ttggtcctgg tagtcaccaa
      541 tcgccgcgac ctcaccgcgg actggctgat actggagctg gaacggcgcg gggccggcta
      601 cgtccgcttc aacacggagg actatccggc gagcacgtcg ctcacctggg agctcgacgg
      661 cccgacgacg ctgtcgatcg gaggcgccga gcaccggctc gagcccccgc tggcgatctg
      721 gtaccggcgc ccgctgccac cgcggctggg ggacgggctg cccggcgacg cggcagagtg
      781 ggcggccggc gaggccgagg cggcgctgac cggcgtctgg cgtacgcggg acgcggtgtg
      841 ggtcaaccac ccggacgcca acgaccgtgc cgggtcgaag ccggagcagc tgcgcacggc
      901 cgcacggctg ggcctggacg tgccgcgctc ggtcgtgacc gacgaccccg aggcggtccg
      961 gcggctggcg gcggaggtcg gcggcgagct ggtctgcaag ccacttcgca gcggccacct
     1021 ctcgatcgac ggcgaggagg gtctcttctt cacgtcgcgc ctgacgagcc tcgacggcga
     1081 gctggatgct ctcgggcccg agccgtacct cttccaggaa ctggtgccca agctctacga
     1141 cgtccgcgtg acggtgatcg gcgaggagac gttcgcgacg cgcatcgaga gccagtccga
     1201 gcaggacgcg cggatcgact ggaggcgcgt cgacgcgggg cgcctcgagc accgggttga
     1261 ggcgctcccg cccgagatcg ccgagcgctg cgtcgcgctc gtgaggcact acggcctgcg
     1321 cttcggcgcg atcgatttcg cgcgcgagcc gagtggtcgc taccgattct tcgagctgaa
     1381 cccgaacggt cagtgggcat gggtggagca gttcacgggc gccccgatgc gctcgtgcct
     1441 ggccgacctg ctgctgcaag gcgatgccgc actggcgtga cgacatcgcc gagctcgcgg
     1501 cgctgctcgg gttcagcgac cggcggccgc cgccgccgga gccggacgtg ttcgaggaga
     1561 agaccgagga actggcggcc aagctcgcgg tcaagcctga gttggccgac gcgctgctcg
     1621 aggaggccaa ggaccatcac gagtacgcca acgagagggc cgagagcgcc gagcgccgcg
     1681 cgaccaccct ccagggcgcg gtggcgatcg cgtcgagcct gacgctggcg ggtgggagtc
     1741 tgctgctcga ctcgtccaag ctccagggga tcgggtggaa gatcgccctc ggactcggcc
     1801 tggtcggagt cacgctctgc ttgatcatgg ccggctaccg cgcggtcacg gcgacctcgc
     1861 ggctgtacca gtgggcggct ccggacccgg tggacatctt cgtgcacgcc gggctcgaca
     1921 gccccgaagc cgtcaaggtc gcgcgcgcgg cggacctcct caaggcgtcc gggatcaacc
     1981 aggcggtcgc ctcgcgcaag gtcgtcgcga tgggcgaggc cgcgatgtgg ttcacgcgcg
     2041 cgctgttcct gatcctcgcg ctggccgtgc tgctcgcggt ctacggggcg gtcggccggc
     2101 acgagccggc caagaaggac gaggccgcgc agggcacgaa gggtgaccgt ggggccgggg
     2161 tccgatcggc gcgcgtggca cgaggttccc gctcctagcg gacgggggcg gccgcggtcg
     2221 tggtcgccct ggggtgccgc gccagagtgt gatctcgccg cctcgcagcg gcgtagaaga
     2281 cccgatgcac acgttccgat gcacccgctg ccgcgcgctc gcctacagcg ccacctcgct
     2341 cgagcgcctg agcggcggcg acctctgcag cgagtgcggc ggcgagctcg agccggccgc
     2401 gcgccagccg gcgttcaggg cctccagggc agcggcggag gcggcatccc gccgctaccc
     2461 tctgcgcccg agcgcccgta gctcagctgg atagagcgtc ggtctacgga accgaaggtc
     2521 acaggttcga atcctgtcgg gcgcgcttcc gcggatgccg gaccgcggag cttgcattcg
     2581 tcgtcacgcg cgcatagcgt ggcgaggtgc cgatcccgtc gagcggtctg aatacatctc
     2641 gcattcagcg aggctttggc agtccaggga ggcggggcgg gtgacacgca tctcgtggct
     2701 ccacctgtcc gattggcacc agaccgtcaa cgggttcggg cgctccgtcg tgagggacgc
     2761 cctgctggac gacctggctg atcgcacggc cctggacgag aatctcgacg agatcgactt
     2821 cgtcgtcttc agcggcgatc tggcctcgaa ggggcacgcg gacgagctca gcgccgccag
     2881 aggtgagttc ctggatcctg tcctcgcccg agtggaggtc gacaggcagc gcctgttttt
     2941 ggttccgggc aaccacgacg tggaccgcga cgccttcgag ctgctgccga aggcgctctt
     3001 ggcgccgttc tcggacgagt ccgccgtcga gaagtggctc acggacgacg agcgacgcga
     3061 cctggtcctg cgtccgacgc gcgcgtactc ggagctcgcg gccgagttcg ggttcgacgg
     3121 caacccggcc tacgccgcac atgggcacct gaatgttcgc ggggtcgagg tgggcctcgt
     3181 gggactcaac tcggcgttga tggccggccg cgatcccgga ccgcgcacgc ccgggcgcga
     3241 cgaggactac ggcctactcg tggtcggtga gccgcaggcg cgcgaggcgc tgcgccacac
     3301 ggagggcgcg gacgtccaga tcgcggtggt ccaccacccg atcgactggc ttcagtcctt
     3361 tgatcgcaac cggctggagc cggcgctcgc gagcacagcg cacttccttc tgcatggcca
     3421 ccggcacctc gcgcattgcg acaaggtcag gggcacctcg ggagaccacg tggtcatccc
     3481 ggccggggcg agcttcacgg agcgatcggc gagcgaccag cgtttcgcga acgcgtacac
     3541 cctggtcacg ctcgacctcg agcggggaac cggcgtcgca tacctacgcc gctggggcga
     3601 cagacggaac aagtggatcg cggacgagga caccacgccc ggaggagcga gctcgttcgt
     3661 gttgccggcc gcgctgtgcg tctctccccc gacgtccccg ggtcgtggcg agtcgagcgg
     3721 ccgtcgccag cagggtcagg acgaccgtac ggatctcgcg cacacgcgat acctccgtgc
     3781 cgtcgtgaag gacaacgccg agctcgagcc gcgcggcgtc agccaggcca gggagggcca
     3841 ggcgctgcca ctcgaggaga tctacctgca gttgagcgcc gtggcagaca agcccgacgt
     3901 cgaccgccgt gtcatgcagg aggaattcga ggaggtgggc cggcagctcg acctgatcga
     3961 ggaccccgcg gagcgggagc gagagttcga acgctgggct cagcaggcga cgctcctcca
     4021 caagaccctc gacatgaccg tccccagtca gaggctccac gacatcgcgc gcgagcagcg
     4081 ggccgcggtc atcctcggag atccgggctc gggcaagacg actctgctgc ggtacgtggc
     4141 tcttaccttc gcccaggcga tcctcgccaa ccccggcgcc gtcctggacc cgaccgatcc
     4201 gtgggatccg ggcagcgcga cgcaggccgg tgacctcggg actgtccgtc tgcccatcct
     4261 cgttcgcgtc tccgagttcg ccgaggcccg acgcggcaac ccgcagcttt cgctcgtcga
     4321 tttcctgacc gactacttca ccgggctgca gatcccggac gcgcacgagc tggcgccgct
     4381 gttccacacc ctcctcgacg cggggcggtg catcgtcctg ctcgacggcc tcgacgagat
     4441 tccggatccg gccgagcgga tagcggttgc catggcggtc cggcgcttca tcgcggcgta
     4501 ctcggagaag ggaattccgg tcgacgcgtc ctccccgcgc gccggcccgc agcacctgcg
     4561 aagcctgctc acggacgcgc gtgtgatcca caccggcaac cacttcctcg tgaccagccg
     4621 gatcgccggg taccacatcg cgccggtcgg gggggtcgag ctgttcacga tccgcgacct
     4681 ggaacaaccg caaatcgagg agttcttggg gcgctggtgc cacgcggtcg cgcgacacgc
     4741 gcccctcggg cgtgagaccg gcgccgccga ggagcgcgcg cggcgggagg tggccggcgt
     4801 gcttcgcgcc atcgcggagt cgcccggtgt ccgccgcctc gcgtccaacc cactgctgct
     4861 gcggattctc gcgacggtcc accagacgaa gggacacctc ccgcagaggc gcgtacagct
     4921 gtacgagacg gcggcgctca cgctgctcca ggactggaac ctcgcgaagg gcgacgcggg
     4981 aaccgcgatc gatgaggacg aggcggtgcg gctactgggc ccccttgctt tccacatgca
     5041 cgaagcgcac gtgacgccgt tcgtgacgct cgccgaggcg cgccggctcc ttgcccgcga
     5101 gcgggcacgc cagagcggcg aggagccgga gaagccgtca gcacgaaccg agcgcgaggt
     5161 caacgagctc cttgagcggg tgcgccagca cgccgggctg ttcctcgaac gcggcgaggg
     5221 gctgtacggg ttcatgcatc tcacgttcca ggagtacttc gccgcacgcg agctgacgag
     5281 cagcgcccgc acggcgaagc gcttgatcct gaagcggctt cacgagcccc ggtggcggga
     5341 gccgattctc ctcgccgtcg gcatcctcag ccgccagttc tacgacgaca ctcgcgaggt
     5401 gctcctggcg attcgagacg cgccggccga gcacgaggac ctgctgcacc gcaacctgct
     5461 gttcgtcgca cattgcatcg gcgactgtgt tgcggcgccc gacgccctgg tccgtgacgt
     5521 ggccggtcgg ctgttggagc tctggtcgga ccgtacgggc agcgggaggt acaagctcct
     5581 gcaacggcag gttcgcgcag ccctggagct cctcgcgggg gaggggcgcg gcgcggtcga
     5641 cgaggcgctc gccgagtcgc tgcgtcgctg tgccgccaac gggggcgctt gcccacccgt
     5701 ggatctcgtc gagcccctga gcgcgcgcgg cgctcccgtg gctccgaccc tccaggccgc
     5761 ggtcggtcag gccgacgcct gcgtacacga gctgatcgat cgcttggtgg cgctgcgctt
     5821 cgcccagagc ccctccaccc ggatccacga cgccatgctg cgcgagcccg cgttcgcgag
     5881 gctcgtcggt ctcctgcgcg tgagcgggtg ggagcatcgg atcgctctcc tgctccgggc
     5941 cgcaggcgat ttcgaggacg aaaacgcgtc tgagctcgcg ttcctgatga ccgctgccga
     6001 ctccgtggac gagattcgca tggcggtcgc cctggctcgt tcgagcgcgc gggcgtcgtt
     6061 cgcgaacgtc ttcctcgcgc ggatgtacaa gagcgcgccg ccggacgcgg agctgaaggc
     6121 cgcgttggac gatgtgagca acgcgctcac ccttccgggc cgggatcgtg cggaagtggc
     6181 gcgcgtcgat gacgccgtag gaggtcttga ggaagcggcc aggcgcgtgc gtgaggaagt
     6241 cctccgtcgc ccggccgcgg acaccgtcga acggttgggg cggcccatgc tcgatgcggc
     6301 ccggccagtc tccgcggtgg tgccgcacct ggccgccgtg ggcgccttgc tgctcggctc
     6361 gggggaggcg ccgcccccgt cggcgctcga cgcgaagcac gcgatcgtcg cgcgcttgca
     6421 caaggcgctg gcggcgaccg gcgatcccct cgagtaccac gacatcacgg cgttcctgct
     6481 ctcgcttcag gacgagggtc gcgagggagt cgtggacgcc tcggcggtcg ccgcgtccct
     6541 cgtgcgggac gcaacgcggt ccggtgcgag gcagcgcgtc gccctcgcga ccatgtgttc
     6601 ggaagaggtc cagtcacacg tccagctgac gcacgaccag cggtacgcgc tgctcagcgc
     6661 ggaagggccg gagggcacgg caatcgctcg cctccaagcg agtgtgcatg ggacgctcga
     6721 tggccggtca cttcgcgtct gcttcgacct gctgtgtcgg tccgatgcgg cgctgcacga
     6781 gaccctcctg tcccgactgg gcaccgcgcg agcgcaggga tcgcgcgcag gactcggcgt
     6841 cctcgagggc gctcttgacg acgcggccgt tcggcccgtc gcccttgacc tcatgaagcg
     6901 aatcgagtgg gtggacggcg agacgctcgc gtggactctg gcggcggtcg agagcgacga
     6961 cttcgaaatg cgcgcccacg ccgccgtgct gctggcacgc caggagcacg tctaccacga
     7021 ggtccggctc gcactcctcc agccacccgc ctcacccggc gtcacagggg acggcgactc
     7081 gtggcggcag ctcgcaaacg agaccacagt tgtgcgcctt ctcgcgctgc tgatgaccaa
     7141 cgggtggcac gacgccctgt cgtacgccct cacgggccat gcggctggtc ataaggacgt
     7201 catcgcacgc ggacacgagt tcaaacgcgc gctccggatc gaacgagacg cagaggaacg
     7261 catccgccgc gcgcttcgat acgaccaaca tgttctgacg aggaggcacc acgcaacact
     7321 cgtcgcggcg ctgcgtggag cggctgaggc ggcgggtttg cgcgacacga ccggctggca
     7381 gaacccggag atcgtgcagc gggcggaatc ggcgctggtc gcgaacatcc gccgcacacc
     7441 gcatctcatt gggatcctgc cgctcggcga gctccgtagg ctcgcgcagg agcgaaccgg
     7501 acgcgtcgcg gactgggtcg cgacggccgt ggcttccgcc ggcggtggcg acgcatccgc
     7561 aagcggcctc gccgcgctgc tccgggcccc gtccgccgcg gtgcgacgcg gagccgcgtt
     7621 gagtctcgtc gccgaagacc tccacgcgaa ggtcatgccg gtcctggcgg aggccatgga
     7681 catcgcggat gaccgcctgc gacgctccgc gcgggacgcg ttcatgtcac tcggcaccca
     7741 gctttcgggc tacaacgaat ccgccgccgt ggactggctt cgctcgaggg actacgacga
     7801 gctacccggc tacgcgagcg gcgccttcgc cgcggccctc ggcgacgtgt actgcgtgcg
     7861 accgcagagg ctcgagcagc aactctccga tgaccgcatc aagttcgact cgatgtcacc
     7921 ggcgctggtc ccgctgctgc ggcgccatct gagcgaggcg agcggttccg ctgccgagga
     7981 ggggcattcg gtgctcgcgg cgatttgcac gaccaacagc gcgccatacg agcctggatt
     8041 tcatcgcgtt ccgctcgacc tgatggactc gacggactcc gacgcacgcg cgctgggcgc
     8101 gtacacactt cgttggagcg ccgacgatga cgcggccgag gcaatcgcgc gcctgctcgc
     8161 ctcggcggaa acgtcggacg acgagtctct cgtcgccgcg gcgcttgggt cggtggggac
     8221 cctcgcggca cgcgcgccga aggggggcga ctcgcacacc gtcgagacgc ttcgcaagtt
     8281 cgcccgccgc gacgccgagc gggtcgcggc ggcggcgcgg atggcgatcg ggcggatcga
     8341 gctcgcacgc gacgtggcgc cggcggacat gacggtcggc acgacggagc aggtcctcgc
     8401 cgccgcgctg ggaggcgtca ccactgcgca gagcggatct aagccacggg tggtcgaggt
     8461 atccaaggcg gtggccggct gggttaccgg gaatgaggag gcctccgccc aaccgtccat
     8521 cgacctgctt ctggactggc tcgacgagtc tctccgcaat aggcccgacg aggggccgac
     8581 tgggggtgag cgggccgcgg gaatcgtgca gcgggagttc ggatggccgt tccggtgcgc
     8641 cgtggcgtgc accctggcag agctcagcga gagactgacc tggaggtcgt tcacggcgaa
     8701 acgcgacctc gtagacgtca tcgatctgct caccgacgcg ggatccgaca ggatgagctg
     8761 gttcgtccgg cagcaggcga tcagggcact tggcaacctg caactgctca cccccgaagt
     8821 cgtgagcgtg ctcttcgacg cgtgccgtga ctcatcggag gtgtacgcgc aggcgcgcac
     8881 cgctgtcacc aacttccggc gcttcagccc gcagacgctc gcgcgcctgt cgcgggccac
     8941 cgccgacgag agcgtgatgg tcgcggaaca cacggcgcgg ctgctcggcg ccctcgggac
     9001 gagcaggtcg gaggagctcg gggccaaggg acggaaggag atagcccgtg agctcgtcaa
     9061 ggtgctgggg tcacctcact gcgagcgagc cgtatacaac gcatctgggg cgaaaacggg
     9121 gcagctgttc gatgttgtgt tcgaggcgct cgtccgtgtg gtagacccga gcgtgatgat
     9181 ccacagtcgg cccgcgccgg gcggcttcga ggggctccct ctggggctta ggtgacgttc
     9241 gggtgcacgg ccggccgggg cctacgtccg tccggcgcgg aggcgtcaga cgagcaggcc
     9301 gaggcgctgg gcgcgctgga tggcgtcggc tcggttcttc gctttcatct tgcgatagat
     9361 cgcgctggcg tggtccttga tggtgtgggg cgagaggaag agcgcatccg cgatctccct
     9421 gttggtcgac ccccgcgcga tcagttggag gacgtcgagt tcgcgctcgg agagaacatg
     9481 ccgcattcgc ttcggctccg gggcgaagac gctcatcccg agtcccacca tccgcgtcgc
     9541 ggaggcgatg tcctggactc gccagccctt cggaacgaac cccgaggcgc cgagctcctg
     9601 cgccgagtga cctgtgatgc ggccggtcgc ggacgtcagc aacacgtgcg tcatgggcga
     9661 gatctcgcgc agcttggcgc acaggttggt gcccgactcg gtaccgagaa gcatctcgac
     9721 gagcgcgacg tgcggctcgt agcggcgggt gacctcaagc gcctggccgg ggttgtccgc
     9781 cgtcaagcag cactcgaccc acgactctgc gatgaagagg gttcgcagcc cccacagcgc
     9841 cagatcctgg tcgtccacga ccaaaacccg aaggcggcgc gccgggtcgc gaacgggccg
     9901 cccggcgacc gtcgcacgca gccctttgaa gtcgactttc accctcgtcg ccgcgatcat
     9961 gcgatccgtc cacgatatgt gcgcatcatc agcacgccga gggcgcgagc gctctggcgc
    10021 ggaggttcgc gcgctgggtc ccactggccg ccgggaaggg ttgccgagcg ggccagcagg
    10081 tacttgctcg cgagacccga ctcctgctcg tcgagatcga cagcgaactc agggtccacc
    10141 tcgagaaggc ggacgaacga ccgccggccg ccggccttga ggtcacgcac cggccgaccc
    10201 tatgcgccgg ccgtggtcag aacatctgcc gtggggagga aatctgcgct cgccgggcgt
    10261 ctccacgcta cccacggacc ccccttcggc tgacctcccg cacgggacta ttcgcgagtc
    10321 ggcccggggc ggccacaagt ttctctcccg ttcgggagat gttgtggggt ccatgttttc
    10381 ctacgatggc cccgtgaagg ggcgcaccgc ccgcctcgct caggaatcct ggattgatga
    10441 gctattttta gctcaggcgg ttgagggggg gtttcgacag gagtcgtgat gacagcgatc
    10501 gcggccgata gtgcgcatgc gcttcgcgcg ggaacgctga ccttcctgtt cagcgatttc
    10561 aaggatgctg tgcggctgag ggacgccttg cccgacgcga tggagcgggc ggtaggtgtc
    10621 caccgcgaga tcgtctcgga ggcggtagcg cggggcggcg gcgtagagct cgcggcggag
    10681 ggcgagggaa gcggggctgc cgccacgttc cgctctccgg cagccgctct gcgcgccgcg
    10741 ctggacgtcc agcgtgccgt acgcgcgcgc gagtggccgg acgggctcgc cccgcgtgtg
    10801 cgcatcgcgc tgcacgcggc cgatctgaag cacggtctcg agcgaaacga cgccgccctg
    10861 gcgctcggcc gctgctcgcg cctcgccgcg atcgcctgcg gcggccagac actggtgccg
    10921 cgcgccatcc gcgagctcgt cgtcgaccgg ctaccggacg gggtcgagct ggtggacctc
    10981 ggcttgcacc ggctgcccga cctgggcctg cctgagcacg tctacggcgt ggccgacggg
    11041 ccccagccgg aagcgcctga cgcgctgcgc tcgctcgaca cactgccgaa caacctcccg
    11101 tacgggctga ccagcttcgt cggccgcgag cgcgagctgg ccgaggtcgc cggcgtcctc
    11161 ggcggagcgc gggtgctgac gctgaccggg gcgggcggct gcggcaagac ccgccttgcg
    11221 ctgcaggtgg cggccgacgc actcgatcgg ttcccggacg gggtctggcg ggtggagctc
    11281 gcgcccgtct ccgacaccga gagggttggc caggcggtgg ccgaggcggt cggcgtacgg
    11341 ccgcatcgcg gccagaccgc gctcgaggcg gcggcggcac acctgaagga gcgacggacg
    11401 ctgatcctgc tcgacaactg cgagcacgtg ctggaggctg cggcagaggc cgcggagacg
    11461 ctgctgaggg gctgtccggg tgcgaccgtc ctggccacga gccgtgctcc gctcgacctt
    11521 tccgccgaga cgacctggcg cgtcccgtcg ctttcacttc ccgaggacgg cagcggcgag
    11581 agcccgcacg cgctggccgc ctcggacgcg gtgcggttgt tcatcgagcg cgcgaccaag
    11641 gtgcggccgg aattcaccga ggcgaccgcg ccggtgctcg ccaagatctg ctctgagctt
    11701 gacgggattc cgctcgcgat cgagctggcc gcggcccgcg tccgcctgct gtcggtcgag
    11761 cagatcgcca cgggcctatc cgaccgcttc cggatactga ccggtgggac gaggaccgcg
    11821 ttgccgcgcc accgcacgct gcgcgcgtcc gtcgattgga gtcacgcgct gctctccgac
    11881 gaggaccgca cgctgctgcg ccggctgggc gtcttcgtgg gcggcttcac gctcgaggcc
    11941 gcggagcaag tctgcgcggt ggacgagcgc gagcgctgcg ccatcatcga cgtgctcgcc
    12001 tcgctcgtcg acaagtcgct cgtcgtcgtc gaggagcgcg gccgggtggc gcgctaccgg
    12061 ctgctcgaga ccgtgcgcga gtacgagctc gagcttctgc gcgaggcgga cgagctcgaa
    12121 gcgatccgcg accgacattg cgacttcttc ctcgcgctcg cggagcgcgt cgcccctgcc
    12181 ctcctgactt cgagcgcgca ccaagagctc gacgcactgg acgaggaccg cgcgaacctg
    12241 gacgcggcgc tcggctgggc gaccgagacc gacggcgagc gggcgctgag actgtgcgcc
    12301 gcgctgatcg ggggcgggct ccagcgcagg cgcttcaccg cccgtgacgc cgcctgtgcg
    12361 cgggcgctcg aaaaggccgg cccagagccg acccggctgc gcgctcaggt cctctccggg
    12421 cgtgtgctgc tgctctttta tgccgggcgc cacgaggagg cgacggcggt ggcgcaggaa
    12481 gccctcgagg tcgccgagga ggtcggcgac cccgctgaga tcgggcgggc gctcgaggcc
    12541 ctcgggaaga acgcgatcgc tacgtcggac ccggctgcgg tgcgtcccct cctgaagcgc
    12601 gcctgcgcgc tcctgcgggc gagcggggat gacttctcgc tcgtcacgac gacgcagacc
    12661 atggcctggg ggcacatgta ccgcggcgag tacgaggagg gcgaggccct gctcgaggag
    12721 gtcttcccca tcgtcgagcg attgggcaac ccggagctcc tgtggtggca ctgggtcggc
    12781 atggcgcttc ggcaccagca cgacggggag accgaacgat atttcgagtt ctgcgagcgg
    12841 gggctcgagg ccgcgaggga gatcggcggc cccggcgaga gctttatcca ctcccagata
    12901 gcgattgtgg agctcgctca gggccggacg gcgcaggccc tcgctcgcct ggaggcgagc
    12961 cgcgagcgag ccgtcgcggc tggtgcaggc ctggccttcc tctcgacgga gctcttgctc
    13021 gcgtcggccg aggccgtgct cggcgacctc gacggcgcgc gggcgcggct cgaggcgctc
    13081 gccgcatcga ccgcggacgg catccaactc ggttggatgg cggcgcagct cgccgacttc
    13141 ctgcgtgtcg gcggcgacgc ggcaggcgcc gaagactggg cccgcaaggg gctccaggtg
    13201 aacgagcggg tggcgttccc acactgcctc agctggtgca aggaggtctt gggccggctc
    13261 gcggccgatc gtggcgactg gagcgaggcc gaggcgttct tccacgaggc cctcgcgttg
    13321 cgcgccgaaa agcagcagcg gctgtggctg gcgcagggcc tagatgggct cgcggtggtc
    13381 gcggcggggc ttgaggcgca cggggaggcg acgcgcctgc tcggtgcctc cgcgcgggcg
    13441 cgctccgacc tcgggaccgt ccgctgggcg ctcgaccaac cgcacttcga ggcgctcgag
    13501 cgagatctgc gcgtcgagct gggcgacgag gcgttcggcg ccgcatgggc cgaggggtcg
    13561 cggctcacca tcgaggaggc ggtggcctgg atccgtcgtg cacggggatc gcgcaagcgc
    13621 ccggcggccg ggtgggagag ccttacgccg acggagctcg aggtgacccg ccacgctgcg
    13681 gaagggctga ccaatgcaga gatcgggaag gcgatgttcg tcaccggcgg gaccgtgaag
    13741 acccacctct cgcacatcta cgcgaagctc ggcctgcgca accgcgcgga gctcaccgcg
    13801 gaggcgacga ggcgccgctc gggcaactcc gcagaggtct aggggggtcg cgggaaccgc
    13861 aggatccgcg gggatcgcgg tgacagcggc aagcgccaga ggtgcagcgt ttgcggggcc
    13921 cagcgaggtc atcacacggg cgcgcttcgc cgctgccagt accctcgctc cggtgagcta
    13981 cgtcgccggc gaggccaggc aggagctgct cgacaccctc ggcgacgcga tcgacgagat
    14041 cgctgccgcg ctcgccgccc tcggtgcggc ctacgagctg ctcagcacgg cccccgccga
    14101 ccggctcgag gaggagctgt tccggcccgt ccaggccgcg tacgggcgtg cgaagaagac
    14161 tcacgccggc ttcgcgcagc ggcagggcat acctccccgg acgttcactc ccgccactcc
    14221 gggtcccccg tcgacgggca tcagggggtt cgtcgacagc gccgtcgact cagccgcgga
    14281 tgccgatgcc gtgctcgtcg agctccagga ctcgatggcg ccggtggagg tcggcgatcc
    14341 cgagctgcgc gccgggctct ccgaggtccg tgagctcctg ggtgacgtgc gcgcgcgtgc
    14401 gcacgagttc gtacgcacgc tcggccgcta gcgcgacctc gtcgccagag gggacctacg
    14461 gcaggttcgc gatgatcgtc ttcgtgaacg tcccgtccgg cagcccggtg ccggacccct
    14521 tccggctctc cacgtcgtac gagtacgtgt agagcatcac gcccttcttg gtgccgccgt
    14581 ccggctccca cttgcagatc gtctcgacct cgtcgatcgg cgtgaagttg gccccgtcct
    14641 cgctcggcgg cgaccccggc ggtcccatgc agctcagccc gatcgcgatc ttctccgacg
    14701 agccgatcgc gcccgcgtac acgtcgaagt tcgagatcgt ctcgtcgacc ccgggccatg
    14761 gcgtgtagtc catcgtcgtc acgaagtcga gctcggccgc gaactggccc aggaactcga
    14821 gctgcgaatc ccacagcccc cagatcggag cggtcaggag cgggtcgcgc ccgagcgcgt
    14881 cggtcagggc cggccggagg gccttcgtca cgtcgagcag cgtctggctc ggctcgaggt
    14941 agctcggctc gtagtcgagg tccaccccgt cgacccccca ctcgaccgcc tgctgcgcca
    15001 ctccgcccac gaacgcgtcc acgtccggca ccgtcccgag cttggtgtcg ttgatcgaga
    15061 acagcacctt cgtcccgttc gcgcgcacgt ccttgatcca cccctggatg accgaggccg
    15121 aattcgtggt gcagaggaag tcgaagtcga gcgcgtactg gtcgtcgatc gtcacgaacg
    15181 cgagcggcgc gacgtcgacc tccggcggca tctcgttcag cccgggcgac ggcggcgtgg
    15241 gctcctgccc gatccagtag ttgacgaagg tctcgttgct cacggaccgt ctcctctcgc
    15301 gactcgggaa gcccgatcct cgcgcctggc gcacggcgcg cgcaaccccc catgtggcgg
    15361 caccgcgcgg ccgcgtctac ggcgcgagct cgtgcttcag caccttgccg agagcattcc
    15421 gcggcagggt gtcgacgaag tgcacgacgc ggggacgctt gaagccggcc agtcgctccg
    15481 cggcgaagtc gacgagcgag tcggccgcgg ggcgctcgcc ggcggggacg acgaacgcgg
    15541 tgaccacctc gccccactcg tccgagggcg tgcccacgac cgcgacctcg gccacgtcgg
    15601 gatggccgag cagcacgtcc tcgacctcgc gcgggtagac gttgaagccg ccgctgatga
    15661 tcagctcctt ggagcgcccg aggatgcgca ggtacccgtc cgcgtcgagg ccgccgagat
    15721 cgcccgtgcg gaaccagccg tcctcgaagc tctcggcgtt ggcctccggc cgttcccagt
    15781 acccgccgaa gacgttcggc ccgcggagct ggatctcgcc gtcctccccg cccgcgagcc
    15841 gcagctcgac gccgggcagc ggaaggccga cgctgcccgc gcggcgctcg ccgtcgtacg
    15901 ggttggagac gttcatgatc gtctcggtca tgccgtagcg ctcgagcacc tgctggccgc
    15961 cgcgctcggc gagcgtgccg tgcagctccg ccggcagcgg tgccgatccc gacacgcata
    16021 gacggagccg cgcgagctcg cccacccgcg gggactgcgc gagacggtgg tacatcgtcg
    16081 gcacgccgaa gaacatggtc gcgtcctggt cgccggccgc gtcgagcacg gcatcgacgt
    16141 cgaagcgggg caggaggacc gccgaggctc ccgccagcaa cgtgccgtgc agcccgacgc
    16201 acagcccgtg ggcgtggaac aacggcaggg cgagcacgag ccggtcggcc gccgtccagc
    16261 gccacgccaa cccgacggac tcactccccg cgagcaggtt ggagtgagtt aggaccgctc
    16321 ccttgggact accggtcgtg cccgacgtgt agccgagcag cgcggggtcg ccggggtcgg
    16381 cggcgtcgag cacggcgggc tcgcgatccg gcaggtcgac ctcgggcccg accaccaccg
    16441 tctcctgtcc ggcggcggcg cggatccagc cggcgcggtc ggggtcgtcc acgagcgcgg
    16501 ccttcggacg cgagtcgctg accacgtgcg tgacctcccg ctcccggtag gcggtgttcg
    16561 tcggcaccac gacgatgccg gagcgcagcg cggccacgtg cgcgacgacc agctccagcg
    16621 aggactcggc gctgaacagc atccggtcgc ccggctcgag ccccgccgcc tggagccggc
    16681 cggcgacccg ccgggtcgcg tcctcgagct gatgcgcgtc gatccagccg cgctcgcgat
    16741 cgtgcaggat cggagccgac ggggcgctcg cccacacctc agcccaggcg gcgggcaggc
    16801 tccccttggc ggtgaggtcg acgtcctcgg cgcgaacgcc cgcgggcagg tgcgcctccc
    16861 agctgacggt catcgcgcgg cactctacgc ccgtgggacg cggtgcggcc gggccgggct
    16921 cagcgcgcga cgtcgcgcgt gaacgccgcg gtgcgcacga tcccgtcgac cttgaactgg
    16981 aggaacaggc ggtagcggcc gtcggtgggg aactcggtcg cgaaggcgat cggtcccccg
    17041 gtggagcccc cgccgtgccc gccgccctcg tgacccgcgc cgccctcgac ggggtgcacg
    17101 tgcaggtagg cgaggtcgcc ctcgcgcagc gccacgaggt gcccgcgcgc gccgaggtag
    17161 ggatcgacct cgacgggctt gccgccgtgc atgacctcga actcgagctc cgactcctcg
    17221 ccggccgacg aggccccgct cgccagcatc acctcgaagc cgccgcccgc cgaggcctcc
    17281 gcgcgcggcg ccgggatcgg ctcccacgcc gtcgcaccgt cgaccgccag gtcggacgcg
    17341 agcgtgtggt tgcggccgtc gcgcttgaag tcggcgaaga cgcgatagct gccggcctcg
    17401 tcaagcgtga gcggcgtcga ccacgtgccg tcggcggcca tggtgggatg gaggtgctgg
    17461 aagccggtca tgtcgcggcg gaccacgatc aggtgcatgc gcttctcgtg ctcgacctcg
    17521 aagtcgcgca cggcggcacc gtcggagccg aggatccggt agctcagccg cgaccccgtg
    17581 ccgcgcggca tctccgcgcg ctccagctcg agcttcatgc cgtcctcgct cacggcgagc
    17641 ccgcggacgg cgtcctgctc ctgctcgtgc ccccgctcgc cgctcatcgc cgagtgccct
    17701 ccgccgtggc cgccgccgga gtcgtcgccg ctcaccaggt cgccgatcag caccgcggag
    17761 ccgaacacga acgcgaccgc cgcgagcgcg ccgaccaccc gcacgtccgg cccgccgtga
    17821 atcccggtct cgtgcaccgc cgaatggccc ttgccgcgtg cgatcagcca gcggttgagc
    17881 gggaaagcga agctccccgc gatcacgagc gcgaccgaga gggaggccca gaacaggagc
    17941 tcgtcgagcc cggcctccat cgcccccggg atcacgacca tgagcgcgtt gtccacgacc
    18001 tccatcaccg cgatgctggc cgtgtccgat gcgagcgcga tcggcaccac cgcgccgagc
    18061 gcgaggcccg cgcgcagcag cggcacgctc gtgagcgtgt agccgaacac gaaggcgagc
    18121 gcgacggcca gaaggatcgt gccgaggttc gagaagccga gcgcggtccc gatgaccatg
    18181 cccaggatct cgccgatcgc gcagcctgtg aggcagtgga gcgtggcgct cagggccacg
    18241 ctggacaggg cgcggccgga ggtcggcatc tcgtggtgcc cgttcatgcc gacccgaagc
    18301 ggcgcagccg cagcgcgttg aggaccaccg acacgctcga gagcgccatc gccgcgcccg
    18361 cgatcagcgg gttgagcagg ccggccgcgg cgagtggcag ggcggcgacg ttgtagccga
    18421 acgcccagcc gaggttctgc ttgatcacgg tcagcgtccg gcgcgagagc cggatcgcgt
    18481 cgacggccgc gcgcaggtcg cccgagacga gcgtcaggtc cgaagcctcg atcgcgacgt
    18541 cggtcccggt gccgatgctg aggccgaggt cggctgccgc cagcgccggc gcgtcgttga
    18601 cgccgtctcc gaccatcgcc accacgtggc cctcggcccg gaggcgttcg acaacctcgg
    18661 ccttgccgga cgggagcacc tccgcgatca cgtcttcgat cccgacctcc gcggcgatcg
    18721 cccgtgcggt cgtctcgttg tcgccggtca ggaggattgg ccgcagtccg agctcgcgca
    18781 gccggcggat cgccccggcg ctcgtgggct tcaccgagtc ggccaccacg agcacggcgc
    18841 gcgccgcgcc cgcccagccg gccacgaccg cggtgcgccc gcgcgcctcg gcgtcgcgca
    18901 gcgccgtcgt gagggcggcg ggaagcgcga gtccgcgctc ggcgagcagc gccgggcgcc
    18961 cgacgatcac ggcgcggtcc tcgaccgtcc cctcgacccc gagcccggcg acgttcgcga
    19021 agtcccgcac gggcgccagc tcgccgagct cgccgcgagc ggcgtccgcg atcgcgcggg
    19081 cgatggggtg ctcggacgcg tcctcgacgg cacccgcgac gcgcagcgcc tccgcccgat
    19141 cgacgccctc cgcgagcgct acgtccacga gggtcatccg gccggtcgtg acggtgccgg
    19201 tcttgtcgag cacgatcgtg tcgacccgcc gggtggcctc gagcacctcg ggccccttga
    19261 tcagcaggcc gagctgcgcg ccgcgcccgg tccccaccat gagcgcggtg ggagtcgcca
    19321 gcccgagtgc gcacgggcag gccacgatga gtacggccac cgcggcggtc atcgcgaagc
    19381 cgaggccctc gcccgcgccg agccagaagc cgagcgcgcc gatcgcgagc aggatcacgc
    19441 ccggcacgaa cacgcccgcg acgcggtcgg cgagccgctg caccggagcc gtccccgact
    19501 gcgcgtcggt gaccagccgg gcgatctggg cgagcgcggt gtccgcgccg acccgcgtgg
    19561 cgcgcacgac gatgcgcccg ccggcgttca ccgtcgcgcc cgcgacctcc gcgccgggct
    19621 cgacctccac gggcaccgac tcgccggtga gcatcgacat gtccaccgcg gagcggccct
    19681 cctcgaccac gccgtcggtc gcgaccttct cccctggacg cacgacgaag cggctgccgg
    19741 gccggagttg ctcgaccggc acgcggcgct cggagccgtc ggcctccagc agcgcggcgt
    19801 ccttggcgcc gagctcaagc agcgcctcga gtgcggcgcc cgcgcggcgc ttggcgcgcg
    19861 cctcgaagta gcgccccgcg aggatgaagg tggtcgcgac cgacgccacc tcgaggtaga
    19921 tctcgtgcgt gccgccgcgc gcgtcgggga tcaggtcgag cgacatcgtc atcccggtct
    19981 ccccggcgtc gccgaggaag agggcgtaga gcgaccaggt ccaggccgtg agcacgccga
    20041 cgctgacgag cgtgtccatg gtcgccgcga agtggcggag gttcgcccac gccgcgcggt
    20101 ggaacggcca ggcgccccac aggacgaccg gggtcgcgag ctggagcacg agccactgcc
    20161 agcgatcgaa ctggagcgcg gggatcatcg acagcgccag gaccggggcc gacaggagcg
    20221 ccgtcacgat cagccgccgg cgcaggggcg cggtgtcgtc gcgctccggc tcgtcggcgc
    20281 cgggctcgtc ctcgggcagc acggcctggt agccgacggc ctccaccgcc gcgacgaggt
    20341 cggtggtctc gacggccgcg gggtcgtagt cgaccgtggc ccgctccgtc gcgtagttca
    20401 cgctcgcgct gacgccgtcg agcttgttga gcctgcgctc gatgcggttc gcgcacgacg
    20461 cgcaggtcat gcccgcgatc ggcagctcga ggtgttcggc ggtctccggc atcaggctac
    20521 gtcgtagccc gcctcgtcga tggccgccac gaccgcgtcg cggctcacct cgccgcgcac
    20581 cgtcacggtc ttcgcgtcga gatccacatc cacgccctcc acgcccgcga cctgcgtcag
    20641 ctcggcggtg atggcggtgc tgcaatgccc acagctcacg ccggggacgc tgtaggtggt
    20701 ggtgtcgctc gtcatctcgg gctcctatac ggggaggggg tatgaccggg gagcggagac
    20761 tagcataccc cccacccgta tgctaggttc gactcccacg ccccctcgga gagaactcga
    20821 atggccactg aatccagcac tcccagccgc ggctacagcg ccacgaagga ccagctcgtg
    20881 aagcgcctgc ggcgcatcga gggccaggtg cgcggcatcg agcgcatggt cgaggacgac
    20941 cgctactgca tcgatgtcgt cacgcagatc accgcgatcc aggcggcgct cgacaaggtc
    21001 tcgctcggcc tgctcgacca gcacgcgcgc cactgcgtcg tggacggcca cggcgagggc
    21061 ggcccggagg agctcaccga cgagctgatg ggcgcggtgg cgcggctgat gcgccgcggc
    21121 tgagccgcgg cgcccgcccg ccgcctagcg cctgcgccgg ctctggctga tctgcgccgt
    21181 cacgcgggtc ttcccgcgta cctcgcgcgc gcgaacgcgt agcttcagcg tccccggctt
    21241 caggccggtg agccggaagc tctggtacgt gtcgcccgcg aagccctgca cgcgcaggcc
    21301 gtgccgcccg ccgcgcttgc cgctcttcag cagccgccgc agcgacttcg cccggatccg
    21361 ctggaccacg tcgccgtcgt cgtcctcgac ggccatctcg tccacgtcga tgtcgtcgtc
    21421 ctcgtcgtcc cagctcacca ccacgtcggc ggagtgcgcg tcctcgtcga gctcctcgtc
    21481 ccactcggcg agctcgtcct cgtcctcgtc ggcctcgggg tcgtcggtga cgatcgcgtc
    21541 gagggtggcg atgtcgatgt cgcagttgag caccgagtcg atcccgttca gcacctgctg
    21601 gagctcaccc tcgtcgaccg acgggaagta gcgcccgccg gtctcgctcg cgatccgctg
    21661 gagccgcccg gcgtcgccgc cgcggcggcc gatgccgagc ccgatcacgt acgtcggcgg
    21721 gccgttcagg tgcccgagcg cgtacgtccc ctcgttgtgg ccgccgtcgg tcaggaagat
    21781 ccgcgcggcg gctcccgggt tctcgtcggc catcacggcg aacgcggcgt tgtagttcgt
    21841 gccgccgttg tcggcctgga gctcgcgctc gatcagcgca agcagctcgt cctgctcctg
    21901 gccggcctcg acctcgagcg gcgggaagag ctggtcggcg gtcgagccga actccaccgc
    21961 gccgagcgtc tcgccctggt tgcgcggctt ggtcagcagg atctcgaggg cgagccggcg
    22021 cagcacctcc gggtcggtcg cctccatcga gcccgagtcg tcgatgatcg cctcgacgtt
    22081 gcgggcggcc acgcacttgt cctcctggac ctcctcggtc tcgcccgggt cgggcggcgg
    22141 agcgtcgggc tcgggccgcg gcccgtagac cacgtaggcc gcaccggtgg gctcgcgctc
    22201 gggcgcgccc gcctccggct cgccgggctc gcccgtggcg gggtcgcgcg ccggcggctg
    22261 gccgttgcgg ttgtagacgc tgccgagcag cacgtccggg cccccgtcgc cgttcatgtc
    22321 gccgattccc gcgaccgagc gcgatgtgac gtcgcccggc tccgcacccg cgatccgcac
    22381 gccgcgcggc ccgagtgccg acaggtcgac atccgagttc tcggcgcggc cgaagaccac
    22441 gtacgacgcg cccgcgttct gccgcgaaaa cgggttggcg agcggggcgg cgatgacgat
    22501 gtcgccgagc ccgtcgccgt cgaagtcgcc cgccgacgcg atcgtcgcgc cggtctggtc
    22561 gccggcgttg gcgccgtcga tccgccagcc gttgccgccc tgcaccgcgt cgagcggcag
    22621 cgagccggcc tccttgcggc cgtagacgat gtagacggag ccggagttgc gcagggcgcc
    22681 gcggtcggcg agctggccgc cgacggcgaa gtcggggatg ccgtcggcgt tcgtgtcgcc
    22741 cacgggtgcg accgcgccgc ccgcggcctc ggtcgagggc tggtcgccgg ggccgccggt
    22801 ctcgccgccg tgcagctcgt agccctggtc gccgaggttg gcgaggtcga ccgtcgcggt
    22861 cgacgacttg ccccagaccg cgtacgcggt gccggcgaag ccgctctcgt ccgggctcgt
    22921 gtacggcgcg ccgagcagga cctcgtcgcg gccgtcaccg ttcatgtccg gcgatcccgc
    22981 gaccgaccag ccggcgttgc cgttctgcgc gccctccagc cggtagccgc cggagccgag
    23041 cgaggcgagg tcaacgtcgg ccggcacctt cgagccgaac accacgtacg cggtgccggc
    23101 gtcggcgcga ccgcccacgt ccacgcgcgg ggcgcccacg agcagctcgc gaaccgagtc
    23161 gccgttgagg tcggccacgc tcgagaccgc gaagccggcg ccgtcgcccg ccgcggctcc
    23221 ggcgatccgg tagcccgcgc cggcgagcgc ggccaggtcg accgggccgg cgcccttctt
    23281 gccgaagacc acgtaggccg cgcccgagtc ggtcgcgtcg gtggcgcggt cggcgttcgg
    23341 ggcgccgacg acgacgtcgg ccaggccgtc gccgttgacg tcgcccgcgg aggcgaccgc
    23401 cgagccggcc tcgtcgcccg cggccgcgcc gtcgatgcgg aagccgccgt cgccgaggtt
    23461 cgcgaggtcg atgggggcct tgtcggtgcg gccgaacacc acgtacaccg cgccggcgcg
    23521 ctcgcgccct gcggcggacg cgcgccgcgc gcccaccagc atgtcggggc ggccgtcgcc
    23581 gttcacgtcg cccgcggcgc ccaccgcgta gccggtctcg ctgcccgcgg gcaggccgtc
    23641 gatcaccacg tcggcgccgt cgagcaggtc gagcgtgccc gattgcggca gcggcgcgcc
    23701 actcaccgcc gtcaccccga cggccgaggc cgcgcacatc agcccgacga tcacgatccg
    23761 gccacgccgc ttcaacattc gattcccccg ttccccgtcc gcccttggcg ggcaatgtac
    23821 gcctccgaac accactttgg cgcattagat gcggtgaccc gcaccgtttt accgcgcgcg
    23881 ccgagcgggt acgggagcgg catggacgac gatcagatgg agcgcttcgg cgaggcggtc
    23941 gagcagaaga agcaggagtc gagggagcgg tcggagcaga cgcgggacga gccgggctcg
    24001 cctggcgccg tcgacggcga ccagccggat ctcgtcgagc agggtcccca ggacgtgcgc
    24061 gacgagcgca agaagaacag cggccacggc aaggtgaccg ccgacaagtg gaaccagtag
    24121 ccccgcgcgg ctactgcggc tggatcaggc ctcgggcgtg aggtagaaca gctcggccga
    24181 gacatcggcc tcggtgtcca tcccgctgac gaacgcgcgg acctggcgcc cggtgatgcg
    24241 ctcgatggcc gctcggaagt ccgcctcgcg cgcgtgctgg aagaacatgc ggacatcgcg
    24301 cagccgctgg tgctcgccga ggtcggccag gctgcgctcg gcaggggtga gcgagtcctc
    24361 cagggtgcag aggatggcgt ccggacccgc gtagctggtg cgcgccttgg tcggcccgcg
    24421 tccgaacagc tccttgtaca tgtgcaccat ctcgttcgag atctgcatct ggagcgagtg
    24481 tgagacctcg cccgggacat cgtgctgctg ctgtgccatg gccgtcctcc cgcgcatccc
    24541 tctgaggtga gccgcgcgga agggggaggg actgagagcc aggcgagcgg ttcgtgcacc
    24601 tccacggtag tcgctctcgc gacccgtgtc ccggtttccg cggcccgcgc gcgggtaccg
    24661 gcggagcgat ggctccgcgc tcgatctgga acggaacgat cgccttcggg ctggtggcgg
    24721 tcccgatcaa gctctacagc gccaccgagt ccaagaccgt ccacttccac gagctccacc
    24781 ggcgcgacga ggcgcgcatc gagcaccgcc gcttctgttc gaaggaggac aaggaggtcg
    24841 acaacgagga gatcgtcaag ggctacgagg tctccgaggg cgagtacgtc gtgctcagca
    24901 aggacgaggt ggccggggcc gacccgagcc gcggcaaggt gatcgagatc gagcacttcg
    24961 tcgcggcgga cgagatcgac cccgtctact acgaccgcgc ctactacgtc ggcgcgcagg
    25021 acgacggcga ggccgcctac cgcgtgctcc acggggcgct cgagcgcgcg gagcgcgtcg
    25081 ggatcgggcg cttcgtcttc cacaacaagg agcagctcgt ggcgctgcga gcgctcgacg
    25141 gcctgctcgg cctgcacacc atgcgcttcg acgacgaggt ggtcgacggc aaggacctcg
    25201 acgtgccgag cccgcagaag aagccgagca agcgggaggt ggagatggcg gggacgctcg
    25261 tctcgtcgct cgagaccaag ttcaagccgg agaagcacga ggacacctat cgcgacgcgg
    25321 tcatgaaggt gatcgaccgc aaggcgagcg gcaaggaggt cgagctgccg gaccgccacc
    25381 atccggagct cgcgcccgac ctgctgatgg ccgcgctcga ggcgagcgtc tcgaagggca
    25441 agggctgacg tggcgcgctc gatctggaac ggctcgctca gcttcgggct ggtgaacgtg
    25501 ccggtcgcgc tgttcagcgc cgtgcgcgac gagggcgtgc acttccgcca gctccacgag
    25561 aaggacggcg cgccgatcga cacgcgccgg tactgctcgg acgaggacgt cgaggtgccg
    25621 tgggaggaga tcgtccacgg ctacgaggtg agcaagggca agtgggtcac gctgagcgac
    25681 gaggagctcg cgtccgcgga gccgcgcaag acgcgcacca tcgacatcga ggagttcgtg
    25741 gacctcgccg aggtggaccc gatctacttc gaccacccgt acttcctggt cccccccgac
    25801 agcgagggag ccgcgcgggc ctatcggctg ctcgtggagg tgatggaggg cagcgacaag
    25861 gccgccatcg gccgcttcgt gctgcgcacc aaggagtacc tggtcgcgct gcgcgtgcgc
    25921 gatggcgcgc tcaccctcac cacgatgctc ttccacgacg aggtgcggcc caccaagggg
    25981 atcgcgccgt cgaaggccaa gaagacggcg ccggccaagg aggagctcga ccaggctgtt
    26041 gcgctgatcg aggcgctgac gacggagttc gacccgtcga gctaccgcga ccgccatcgc
    26101 gagcggctgc tgaagatcat ccgcgacaag aagaaggggc ggaagatcaa gctgccggag
    26161 cccgacggcg agccggaggc gatcccggac ctgatggccg cgctcgagga gagcctggcg
    26221 aaggcgaagt cgaaggcgaa ggcgtagggc gaggccggcg gagctggcgt gcgcggcccg
    26281 cacggtccgt acgatacgcc tgcatggccg gtctgaacgg gggtgatccg gccgctgcgg
    26341 gaaggattcg cgcggcggcc atggaggcgt tcgcggcgta cggcgacgcc gtgtcgatcc
    26401 gcgccatagc ggacgccgcc ggggtctccc cgggccaggt ccagcaccac ttcccgacga
    26461 aggccgagct gcgcgaggcc gtcaacgagc acgtgctctc gatcgcggcg ggcgcgttcg
    26521 ccggcctcga cggcgagaac ggcgacgtgt tcgagaacct cgcgcagcgc gtcacgagca
    26581 tcatgcgcga gcaccccgac gcgctcctct acgtcgcgcg cgcggccgtg gacggcgacc
    26641 cgggcggcct cggcatcttc gacgggttca tggcggtcgc gacgacccag ctcgaggggc
    26701 tcgcggccga cggccggcta gaccccgacc tcgacctcgg atgggccgcc ctgcacgtcg
    26761 tgatcttcaa cctcgccacc gtgctgttcg agcacgcgat cgagagccat ctccccgagc
    26821 cactgcgctc gccggaggga gtcgcgcgct ggcacgcggc cgacaccgag ctgttccggc
    26881 gcggcttcct gcgcgccaag gcagcgcgcg gctagcgctg cgcgccgccg ccctcctacc
    26941 gcatggacaa acgccgtccg cgatttttgg acacccgcac aagggcgcac gacccccgct
    27001 ggcggtagct tccaggcgac acttgaatcc agcacccgta cgcgcgcctg gcctgcccgg
    27061 gcgcgctccg cgtcaggagc cgcctctatg ccgaagattc gccagcacaa cgtcaccgca
    27121 gccaggtgga gcgccaacgg cgggagcctg ggcgcggtcg acctgaccaa ggacgcgagc
    27181 tacccgttcg ggcagaacgt cttctcgatc gaggttcagc gcgagcgcct gccgcaggac
    27241 gtctgcgatc gcctccaggg caccctcgag aagggcgccg ccctcgatcc ctcgctcgcc
    27301 gacgaggtcg cgcgcgggat gaaggagtgg gccctcgagc ggggcgccac ccactacacg
    27361 cacgtcttcc agccgctcac cggcctcacg gccgagaagc acgactcgtt cttctccccg
    27421 accggcgacg gcagcgcgct cgcgaagttc tccggcaagg agctgatcca gggcgagccc
    27481 gacgcgtcct cgttcccgac cggcggcgtg cgcgcgacct tcgaggcccg cggctacacg
    27541 gcctgggacc cgaccagccc ggccttcatc ctcgagaacc cgaacggcgc cctgctctgc
    27601 atcccgacgg cattcgcgtc gtggaccggc gaggcgctcg acgcgaagat cccgctgctg
    27661 cgctcgatgg acgcgctgtc gcgctccgcg atccgggctc tgcggctgct cggcgacgac
    27721 caggccgagc gcgtgttcac cacggtcggc ccggagcagg agtacttcct cgtcgacgag
    27781 cagtacttct tcgagcgccc cgacctcgtc acgaccggcc gcacgctgtt cggcgcgcag
    27841 ccggccaagg gccacgagct cgacgaccac tacttcgggg ctatccccga gcgcgtgctc
    27901 gcgttcatga tggactcgga gctcgagctg gcgaagctcg gcgtgccggt caagacccgc
    27961 cacaacgagg tggcacccgg ccagtacgag atcgccccga tcttcgagaa ctcgaacgtc
    28021 ggctccgacc accagcagct cacgatgcag gtgctccaga acgtggcccg caagtacggc
    28081 ctcgtctgcc tgctgcacga gaagccgttc gcgggcgtga acggctcggg caagcacaac
    28141 aactggtcga tgggcaccga cacgggccac aacctgatgg agcccggcga caccccggcg
    28201 gagaacctga acttcctgtt cttctgcgcc gcggtggtca aggcggtgga caagcaccag
    28261 ggcctgctgc gcgcgtcggt cgcgaacatc ggccaggacc accggctcgg cgcgaacgag
    28321 gcgcccccgg cgatcatctc gatcttcctc ggcggcgagc tggagaggat cttcgacgcg
    28381 atcacgagcg gcgaggggga cccgaacacg ccggcctcgt tcctcaacct cggcaccgac
    28441 gtgctgccgc cgctgccgaa gcacggcggc gaccgcaacc gcacctcgcc gttcgcattc
    28501 accggcaaca agttcgagtt ccgcgcgctc gggtcgagca tgtcgctcgc gttcccgaac
    28561 accgtgctga acacgatcgt ggccgaggcg atcgacgagc tggccgacca gctcgagccg
    28621 aagctctcgg gcggcgacct cggtgcggcc gtgacggagg tcgtgaagga cgcgtactcg
    28681 gggtcgaagc ggatcatctt cggcggcgac aactacgccg acgagtggca cgccgaggcc
    28741 gagtcgcgcg ggctgctcaa cctgcggacc acgcccgagg ctctgcccga ggtgatctcc
    28801 gagcagacga tcggcacgtt cgagaagtac gacgtgctga gccaccgcga gctcgactcg
    28861 cgcttcgagg tctgggtcga gcagtacacg atccgggcga acatcgaggc ggagacggcg
    28921 gccacgatcg cgcgcacgat gctgctgccg gcggcgttgc ggcacatcgc gctgatcgag
    28981 gcggcgggca tcgacacgct cgcggccgag acgcgcgcgc tgaccgagcg gttcgccaac
    29041 tcgattgccg acctcgaggc agcgaacgcc taccccgacg gggtggaggg catccagctc
    29101 gcagagtacg cccgcgacag ccagctcacc gcgctcgccg cggtgcgcga gatcgcggac
    29161 ctgctcgaga aggtggtggc cgacgacctc tggccgctgc cgaagtactc cgagatcctg
    29221 ttcatcaagt agccgctgcg gcggggcgtc cgggcgggcg ccccgctact gctactgcgt
    29281 tttctggcac tcgggagcag cggcggccgc ctggacctct ggcggaacgt cctcgtcggc
    29341 cacgcgtttg cgggtcttct cgaacttctc cggggtgtcg tagccgtgct tcttctcgag
    29401 ctggtccgcg atgcagcggc acagcgcgag gtccttgtcg tcgccctcgt ggcccttgtg
    29461 gcagtcgtcg accagcgcct tcttggcgtc agcgagcgtg gcggggctgt cgctgccgtc
    29521 gccgtcctcg ccgcagccgg cggcgatcga cagcatgaga gcgcagagga tggcgagcag
    29581 cctgggcgtc acggtgagaa gaagttcgcc aggagggcgg cgatctcctc cggcgcctcc
    29641 tcctggagga agtgcccgca ctgcggcagc gccgtgacct cggcgccagg cacgtcgcgc
    29701 ttcacgcgcg ccatggtctc ggcgacgtcc ggcagga
//
LOCUS       DASVPL010000296        11730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_190986, whole genome shotgun sequence.
ACCESSION   DASVPL010000296 DASVPL010000000
VERSION     DASVPL010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11730
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190986"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VF517_16655"
     CDS             complement(<1..655)
                     /locus_tag="VF517_16655"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX8054619.1"
                     /translation="MAGPERLRELLERLGPTFVKVGQYLALRPDLIPQEYCDELLRLL
                     DRVPPFPWEEARAIIEDELGEIGHAFAEIDAKPLAAGSLSQAHRARLHGGGEVVVKVQ
                     RPGVREAVRRDLGRARTIARVLELARVPLVVEPRELVTELRAWMLQELDLERELANMQ
                     KLRELAADSEIMRIPRAYPDLSGKRVLTAEYIHGMPLSTLLGSLRTGREDPGSLSART
                     "
     gene            747..884
                     /locus_tag="VF517_16660"
     CDS             747..884
                     /locus_tag="VF517_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054620.1"
                     /translation="MRAAVWRGALFGCDPANRTARSYFVSDNLKRWAPLPICGVTPCT
                     S"
     gene            872..2071
                     /locus_tag="VF517_16665"
     CDS             872..2071
                     /locus_tag="VF517_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054621.1"
                     /translation="MHVLIVLAPIRPEKESRLREVLTEMQSDPAGNPYLRLGESPSTH
                     FARWVIVDEEDTGPRLLFVANYDGVIGPYLDELVAAGPGMDRLWSACEGYAGKAHFQS
                     FVREHYLKPRGVYIAFPGRTVEQIHNAIAIRQQIESFLDLPEVGSYLSGIGIERFLQR
                     LEQQPKSIPPADRVARAVNDIAANVGGAVHATALRWFLDFAAAFARMGQAKTWPKVHG
                     GCSPEPQRRRETEQAIQGVPVSGDVVQNGMTTVTHIRPEKLARARLALLGTSALSRFG
                     WPPGEFANVGTLHWFAWALPDNGKRLLFISTFDGSWQNYMQDFMDKLVWGLDALYGNT
                     DGYPAAGMKDTAAFTDYILAHQYAPQVVYGAYPQESVMNLIRDEDIASAAPGPAMPAV
                     RRWLQWL"
     gene            2062..3606
                     /locus_tag="VF517_16670"
     CDS             2062..3606
                     /locus_tag="VF517_16670"
                     /inference="COORDINATES: protein motif:HMM:NF044706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054622.1"
                     /translation="MAVSNGGGGTARVAVSDAPSVAAALLPEADRREIQGMITTGWGH
                     LPRAGFVFLRLGEPQRGRDWLQTILPQVTTAAPWTKDESGAKLRPESVLNVAFSYAGL
                     ERLGLPEEGLATFPTEYAVGMPARAAILGDHGESAPSRWQFGGSETEPLHAALVVYAT
                     DEGTLDQRLEELRADIASHDLEEVRVERGARHDRSREHFGFANDGISQPGIEGVRSED
                     VPGPYVVRTGEMILGHLNERDVYPVSPAVAARHDPKGLLPEFPGGALPDCRDLGRNGS
                     FLVYRKLEQDVAGFWRFLQAHCSAAGSGRTRLEHEMVELASKFMGRWPTGAPLVLSPD
                     SDDPSLAEANDFMYRADDPTGLSCPIGAHIRRANPRDSQLHISDTPDESMRATNQHRI
                     IRRGIPYGPFLYPEREIELGNAPVDLEGDGEERGLHFFALNVNTKLQFEFVQQTWIDN
                     AQFCGLFDEKDPIVGDNDGTRGMTIQRLPVRDAVRGIPRFVHVRAGGYFFLPGGSALR
                     YLAELG"
     gene            3621..4595
                     /locus_tag="VF517_16675"
     CDS             3621..4595
                     /locus_tag="VF517_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054623.1"
                     /translation="MPRLDGDSGEYLRTLQSEGYRLAPAATKRDIVWDIVERTRYGTL
                     PKIEFSGFSVVLKALYIQILRRPYLGEYDVRPPRRKLFHPFGVVTKVRFDADGDSPYT
                     GLFATGATGLARLSLAMNEEAYVSGMALKLFVDGQPSQNIAAVAGLDPQPARDFFAPP
                     IETIIPAPRHPPFSNGWSLAYWWLMFVADPLAQTVDKVATVERSGATVPEPHAPHKLV
                     FRPPEGLRTGPDPSDDFRDLLARIPPETEIYRVFAEPGENGGEEVYVGRVTTESEVIA
                     SSFADGMLSFRHERHPARYERRYRWLKLATVALVILVVVAIVSALAAC"
     gene            complement(4598..5038)
                     /locus_tag="VF517_16680"
     CDS             complement(4598..5038)
                     /locus_tag="VF517_16680"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX8054624.1"
                     /translation="MPHTLNAWAPVPIRVIAGSCLAYRGGPELFSRHAHENIVHLTRE
                     MGGPAPEVMAWPIAALQLFGGLGLIGGVRVRPIAGAVLGMMGFHLFQGLRRGFAQSLP
                     GCQPPPSPEAALLFCGCSAALLLTGPGRWSLRPTAADGAVSRLI"
     gene            5652..>11730
                     /locus_tag="VF517_16685"
     CDS             5652..>11730
                     /locus_tag="VF517_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054625.1"
                     /translation="MVEATAPGLSVELAVYEGDVVFDLRRRTQAEAWSTRVKFTARAG
                     VTYHVAAFADRGPPDGAFELRLDLGDTPANDSFASSAPLSGDSPTVAGDTTWSTIEAG
                     EPRPDYTHGTGSNWYTWTATQDGGVTIATGGSELDATVGVYRGDALGRLDHVATDPDP
                     DADDPDGRLSFRATAGVTYRIAVNGTDTQDAGRYSLSLLARPVPPNDAFDAPTPLGSA
                     RSVAVAGRNDGAVAEPFEPAHFFYMHIRPPGPSVWYEWTAPANGSLTVRTDGAGTPLA
                     YTGATLIDLERVRSESAGHHSDGSWVRIRAQAGVTYRIVVFSRWDAGDFVLSLNLRDP
                     PGNDDLANPTELTGSPAQTAGTTTDATAQPCEPAPGVEPLEETSAWYSWTAPASGPVT
                     IDATSGSFEPLLGIYAEAPACERYGGAPAPRTFRATAGHRYWIAVAAWDGRGGEFDLS
                     IDFVAPPPNDRFDDARPMAGSFLDERGTLMGATVEPGEPSPAGEVGSSVWYSWTAPTT
                     GETDITFESWPHPARIAVHTGDEVDELTQVAVGGSVRFRSIEGTTYRIAVDGGAVASY
                     LDFTIRLTHRQSPANDAFANAESLAGIAAETSGTTAGATREPNEPETGLRGATVWYSW
                     TAMSDGGVTVDVDTPDSAVGVYTGDDVATLAQVTRARSSFDPLRVQFRARAGTTYRIA
                     VDRADEYRWGAPFKLSLKLRPLPPNDAFALATPLAPAPAASASGTTVAATAEAGEPAG
                     GDGQSVWYSWTAPAGGSLTLEATGDRPVKIAAYTGSSVSALTPMPVEELRGGGWTRVR
                     VEAGVTYRIAVWTTRTPADFDLSLRLAQRPANDDFAEAAVLSGSEAGTSGTTVDATRE
                     RCDYPSETVWYRWTAPHTGGVILDTAGSETPVRVDVHTGDSACSLEDASPALLSDGGT
                     TERVSFRAVASTTYRIAVHPLWDRTGPFQLDLRLHAPPPNDAFADAIELTGSSDAVTG
                     TTFGATEELGEESRAASVWYTWTAPSGGLAEVGFPPAGRVPGWNVRVYTGDAPGALTH
                     VATSNSNGLAHFNAMAGRTYRIAVRGFGGTILEPFRLYLATAGAPPNDGFAAAERIDG
                     DSGEVRASTLGAGSDPGEPEGPGWRQRASVWYLWTPARSGLAKLELPSEDRAVTGVYR
                     DSPMNQLSLVASGGRTVGLRVVAGTTYRIGVYGRQSVSYGAFKLRWRTLPAPANDDFA
                     NAAALSGESVDHYGSTIGADSEPGEPPPPTQSEGGEASVWHAWRAPASGLVSVRTTSG
                     AADVFTGSDMGSLQRLTVAPDWAETAYFRAVAGTTYWIRVSNDLTATTYGDFRLRLAL
                     AQPAPNDDFERAQQLVDRAVGTTAGAGDQPGEPLRDQESRATVWYTWTPETTGKGRLA
                     FARSSRGLSVYTGARLDTLRRVTSASHGDAAEFRAIAGTTYHVAVDDWLGIAGGFVLD
                     AAVLPGPPNDDLAQATVLTGGSASATGPLANATLEPGEASTTDNYAESSTWYRWTAPS
                     NGRVTFEASRRTILAATGTSIGDLKRLAQSSGRVSVPVRAGTTYLFGLYGSAEQEGDV
                     EVALSHSPAPANDEFANAEALQDWPARASGSNQSATREPGEPRSTGGDDDRSGTVWYS
                     WTAPQNGWAELDLTGTAFAPTAGVYTGDTLAGLSKVAAASAGAGEAPPKLRWRIAAGV
                     TYRIAIGGVRGAAGSIELAIRKVETRPNDDFADAEVLSGRSARVPGSNVGATTEPGEQ
                     GRYGPGYSVWYRWTSEVTGNVTVHAANTDARLWLEAFTGTELQSLSSVSAGSQRVSFY
                     AVAGTTYTVRVAADIYVPMTGSYELRLDQVAAPANDDFAAPSTLEGASSTTTGTTVGA
                     TPQGCDPIELGNGSAPSVWYSWTAPASGAVTLTTGDGVSPPFLGVYTGTEHCWLSAVP
                     TSRPNASTVTFAAQAGVRYRIIVSARRGATGPFALALSVAPSPPPPPPDEPRNPPGDP
                     PAPPDSGSGDQPSPPDPGPADPPSAPDPGPLAPADPPADSRPSPERSAPTEPRTSPAE
                     PSPGGDPAPA"
BASE COUNT         1726 a         4275 c         4152 g         1577 t
ORIGIN      
        1 ccgtccgggc gctcaggctt ccggggtcct cgcgccccgt cctcagcgac ccgagcagcg
       61 tgctgagcgg catgccgtgg atgtactccg ccgtgagcac gcgcttcccg ctgaggtccg
      121 gatacgcgcg cgggatgcgc atgatctcgc tgtccgccgc gagctcgcgg agcttctgca
      181 tgttggccag ctcgcgctcg aggtcgagct cctggagcat ccaggcgcgc agctcggtca
      241 ccagctcgcg cggctcgaca acgagcggta cacgcgcgag ctcgagcacc cgcgcgatcg
      301 tccgcgcgcg gcccaggtcg cgccgcaccg cctcgcgcac gccgggccgc tgcaccttga
      361 cgaccacctc accgccaccg tggagccggg cgcggtgcgc ctgcgacagc gagccggccg
      421 cgagcggctt cgcgtcgatc tcggcgaagg cgtggccgat ctcgccgagc tcgtcctcga
      481 tgatcgcgcg cgcctcctcc cacgggaacg gcgggacccg gtcgagcagc cgcaggagct
      541 cgtcgcagta ctcctgcggg atgaggtcgg gccgcagcgc gaggtactgc ccgaccttca
      601 cgaaggtcgg gcccaggcgc tcgagcagct cgcggaggcg ctcggggccg gccatcgggc
      661 agagcgtacg cgcgcccctc gcgcgcgcgc ccgcgcgcga gagagattgc agcttttgtt
      721 cagcccgggc gcgtaggagc acccggatgc gcgcggcggt gtggcgtggg gcgctcttcg
      781 ggtgtgatcc ggccaaccgc accgcaagga gctacttcgt gagcgacaac ctgaagcgct
      841 gggccccgct gccgatctgc ggagtgacgc cttgcacgtc ctgatcgtcc tcgctccgat
      901 ccggcccgag aaggagagca ggctgcgcga ggttctgacc gagatgcaga gcgatccggc
      961 cggcaacccg tacctgcggc tcggcgagag cccgagcacg cacttcgcgc gctgggtgat
     1021 cgtcgacgag gaggacaccg ggccgcgtct cctgttcgtg gcgaactacg acggcgtgat
     1081 cgggccgtac ctcgacgagc tcgtggccgc gggccccggg atggacaggc tctggagcgc
     1141 ctgcgagggc tacgccggca aggcgcattt ccagtcgttc gtccgcgagc actacctcaa
     1201 gccgcgcggc gtctacatcg ccttcccggg gcgcacggtc gagcagattc acaacgcgat
     1261 cgccatcagg cagcagatcg agtcgttcct ggacctgccc gaggtcggct cgtacctgag
     1321 cggcatcggg atcgagcggt tcctccagcg gctcgagcag cagccgaagt cgatcccgcc
     1381 ggcggatcgg gtggcgcgcg ccgtcaacga catcgccgcc aacgtcggag gcgcggtgca
     1441 cgcgacggcg ctgcgctggt tcctcgactt cgcggccgcg ttcgcccgga tggggcaggc
     1501 gaagacctgg ccgaaggtcc acggcggctg ctcgccggag ccgcagcgca ggcgcgagac
     1561 cgagcaggcg atccagggcg ttcccgtgag cggcgacgtg gttcagaacg gaatgaccac
     1621 ggtcacccac atccgcccgg agaagctggc ccgcgcgcgc ctcgccctcc tcgggacgag
     1681 cgccctgagc cggttcggct ggccgccggg cgagttcgcc aacgtgggca cgctgcactg
     1741 gttcgcgtgg gccctgccgg acaacggcaa gcggctgctg ttcatcagca ccttcgacgg
     1801 ttcctggcag aactacatgc aggacttcat ggacaagctc gtctggggac tggatgccct
     1861 ctacgggaac acggacggct acccggcggc ggggatgaag gacaccgccg cgttcacgga
     1921 ctacatcctc gcccaccagt acgcgccgca ggtcgtgtac ggcgcctatc cccaggagag
     1981 cgtgatgaac ctgatccgcg acgaggacat cgccagcgcg gcgcccggcc cggccatgcc
     2041 ggcggtcagg aggtggctcc agtggctgtg agcaacggcg gcggggggac ggctcgggtg
     2101 gccgtgagcg acgcgccgag cgttgccgcc gcgctcctgc cggaggccga ccgccgcgag
     2161 atccagggga tgatcacgac cggctggggg catctgcccc gcgcgggctt cgtgttcctg
     2221 cgcctcggtg agccgcagcg cgggcgcgac tggctgcaga cgatcctgcc gcaggtcacg
     2281 accgccgcgc cgtggacgaa ggacgaatcg ggcgcgaagc tgcgccccga atcggttctg
     2341 aacgtggcgt tcagctacgc cgggctcgag cgactcggcc tgccggagga gggcctcgca
     2401 acgttcccga ccgagtacgc ggtcgggatg cccgcgcgtg cggcgatcct cggcgaccac
     2461 ggtgagagcg ctccgtcgcg ctggcagttc ggcgggtccg agacggagcc cctccacgcc
     2521 gcgctcgtcg tctacgcgac ggacgagggc acgctcgacc agcgcctcga ggagctgcgc
     2581 gcggacatcg cgtcgcacga cctcgaggag gtcagagtcg agcgcggcgc gcgccacgac
     2641 cgcagccgtg agcacttcgg cttcgccaac gacggcatct cgcagccggg gatcgagggg
     2701 gtccgcagcg aggacgtgcc cggtccgtac gtggtgcgta ccggcgagat gatcctcggt
     2761 cacctgaacg agcgcgacgt ctatccggtc agcccggcgg tcgcggcgcg ccacgatccg
     2821 aaggggctcc tgccggagtt ccccggcggc gcgctgcccg actgccgtga cctcggccgc
     2881 aacggctcgt tcctggtcta ccgcaagctc gagcaggacg tggcgggctt ctggcgcttc
     2941 ctccaggcgc actgctcggc cgccgggtcg ggccgcacgc ggctcgagca cgagatggtc
     3001 gagctcgcct ccaagttcat gggccgctgg ccgaccggcg cgccgctggt cctgtcgccg
     3061 gactccgacg acccgtcgct cgccgaggcg aacgacttca tgtaccgggc ggacgacccc
     3121 accggcctct cctgcccgat cggtgcgcac atcaggcgcg ccaacccgcg cgactcgcag
     3181 ctccacatca gcgacacgcc ggatgagtcg atgcgggcca cgaaccagca ccggatcatc
     3241 cgccgcggca tcccgtacgg cccgttcctc tacccggagc gcgagataga gctgggcaac
     3301 gcgccggtcg atctcgaggg cgacggcgag gagcgcgggc tgcacttctt cgcgctcaac
     3361 gtcaacacca agctccagtt cgagttcgtc cagcagacct ggatcgacaa cgcgcagttc
     3421 tgcggcctgt tcgacgagaa ggacccgata gtgggagaca acgacggcac gcgcgggatg
     3481 acgatccagc gcctgccggt gcgcgacgcc gtgcgcggca tcccacgctt cgtgcacgtg
     3541 cgcgcgggcg ggtacttctt cctccccggg ggtagcgcgc tgcgctacct ggccgagctg
     3601 ggctagcggc ttcgtccgtg atgcccaggc tcgacggcga cagcggcgag tacctgcgca
     3661 ccctccagtc cgagggctat cgcctggctc cggcggcgac caagcgcgac atcgtgtggg
     3721 acatcgtcga gcggacgcgc tacgggacgc tgccgaagat cgagttcagc gggttctcag
     3781 tcgtcctcaa ggcgctctac atccagatcc tccgcaggcc gtacctcggt gagtacgacg
     3841 tacggccgcc gcgccggaag ctcttccatc cgttcggcgt ggtgaccaag gtgcgtttcg
     3901 acgccgacgg cgactcgccg tacaccggcc tgttcgcgac cggggccacc gggctggcgc
     3961 gcctgtcgct cgcgatgaac gaggaggcgt acgtctcggg catggcgctg aagctgttcg
     4021 tcgacggcca gccgtcgcag aacatcgcgg cggtcgcggg cctcgatccg cagccggcca
     4081 gggacttctt cgcgccgccg atcgagacga tcatcccggc tccgcggcac ccgccgttct
     4141 cgaacggctg gtcgctggcg tactggtggc tgatgttcgt ggccgatccc ctggctcaga
     4201 cggtcgacaa ggtcgcgacc gtcgagcgct cgggcgcgac ggtgcccgag ccgcacgccc
     4261 cgcacaagct cgtcttccgg ccgccggagg gcctgagaac ggggccggac ccgtccgacg
     4321 acttccgcga cctgcttgcg cggatcccgc cggagacgga gatctaccgg gtgttcgccg
     4381 agcccggcga gaacggcggc gaagaggtct acgtgggccg cgtcacgacc gagtccgagg
     4441 taatcgcctc gtcgttcgcc gacgggatgc tctcgttccg gcacgagcgc cacccggcaa
     4501 ggtacgagcg gcgctaccgc tggctgaagc tggccacggt ggcgctggtg atcctcgtcg
     4561 tggtcgccat cgtctccgcg ctcgccgcgt gctgagctca gatcagccgc gaaactgccc
     4621 catcggctgc ggtgggccgc aacgaccagc gcccggggcc ggtgaggagg agcgccgccg
     4681 agcagccgca gaagaggagc gccgcctccg ggctcggcgg cggctggcag cctggaagcg
     4741 attgcgcgaa gcccctccgc aggccctgga agagatggaa gcccatcatc ccgagcaccg
     4801 ctcctgcgat gggccggacg cgaactcctc cgatcagccc caggccgccg aagagctgca
     4861 gcgccgcgat cggccaggcc atcacctcgg gggcggggcc gcccatctca cgcgtcaggt
     4921 gcacgatgtt ctcgtgggcg tgccgcgaga acagctcggg ccccccgcgg tacgcgaggc
     4981 agctcccggc gatgacacgt atcggtaccg gcgcccaggc attcagggtg tgcggcatgt
     5041 gcgttctcgc tttcgtcgac ctggcctgcg agccgatttc acccgaattc gacccgtgtt
     5101 cgcgagcgtc gcgcgcatcg gtgtgcacag cgcggacgca ctgaacaaaa gctgaacgtc
     5161 tgcccccggg ggcggttgcg tcaccaccgc ggcgacgggc ttcacggggt tcggcgcgct
     5221 ggtcgctgtg gcgacgtagt cggggcgacg cagggccgcc gacgccggga cgctgcgtcc
     5281 gaagctgagg gttgagccca tctggcgaca gttacctcaa ataggcgatt acgcgggcac
     5341 gcgttggggt ctaccgttgc gtcaccatgc cggggggctc gcaatccgac ccgtgaggta
     5401 acgccgtcct gcgcactctg gcccgacgtg cgctcctcgt cctgacggca ttccttctgc
     5461 tgccggcggc tttcgcacgc gccgcgccgc cccccaacga cgacttcgcc gacgccgagc
     5521 tgctcagcgg cgcccccgta tccacgactg gaaccctcgc ggacgccacg gccgagcggg
     5581 gcgagcccaa ccacggcggc ttcgcacgca gcgtctggta ccgctgggtc gcgccggccg
     5641 acggcactgt cgtggtagag gcgaccgccc ccggcctcag cgtcgaactc gcggtctacg
     5701 agggagacgt ggtgttcgac ctcaggcgtc gcacgcaagc cgaggcctgg tccacgcggg
     5761 tgaagttcac cgcccgtgca ggcgtgacgt accacgtcgc ggccttcgcc gatcgtgggc
     5821 cgccggacgg cgcgttcgag ctgcggctcg acctcggtga cacgccggcg aatgactcct
     5881 tcgccagctc ggcgccactc agtggggaca gcccgaccgt cgccggcgac accacgtggt
     5941 ccaccatcga ggcgggcgag ccgcgccccg actacacaca cggcacgggc tccaactggt
     6001 acacgtggac cgccacgcag gacggcggcg tgacgatcgc gaccggcggt agcgagctcg
     6061 acgccacggt gggcgtctac cgcggagacg cgctcggacg cctcgaccac gtcgccacgg
     6121 accccgaccc cgatgcggac gacccggatg gacggctcag cttccgtgcg accgccggcg
     6181 tcacgtacag gatcgccgtg aacggaaccg acacgcagga cgcgggccgc tacagcctga
     6241 gcctgctcgc ccggcccgtc ccgcccaacg acgccttcga cgcaccgaca ccgctcggct
     6301 cggcccgctc ggtcgcggtc gcggggcgca acgacggcgc cgtcgcggag ccgttcgagc
     6361 ccgcccactt cttctatatg cacatccggc cacccggccc gagcgtctgg tacgagtgga
     6421 cggcgcccgc gaacggctcg ctgacggtgc gcaccgacgg cgcgggcacg ccactcgcct
     6481 acacgggagc cacgctgatc gacctcgagc gtgtccgcag cgagagcgcg gggcaccact
     6541 cggatggatc gtgggtgcgc atccgggcgc aggccggagt cacctatcgg atcgtcgtct
     6601 tcagccgctg ggacgccggc gacttcgtcc tctcgctcaa ccttcgcgac cctcccggaa
     6661 acgacgacct cgcaaacccg accgagctca ccggatcgcc tgcacagacc gcggggacga
     6721 ccacggacgc gaccgcccag ccgtgtgagc cggcacccgg tgtcgaaccc ctggaggaga
     6781 cgtccgcctg gtactcgtgg accgctcccg ccagcggccc ggtcacgatc gacgcgacgt
     6841 ccggttcgtt cgagccgctg ctcggaattt acgcggaggc gcccgcgtgc gagcgctacg
     6901 gcggcgcgcc cgcgccgcga accttcaggg cgaccgcggg ccacaggtac tggatagccg
     6961 tggccgcgtg ggacggtcgt ggcggcgagt tcgacctctc gatcgacttc gtcgcgccac
     7021 ctcccaacga ccgcttcgac gacgcgcgcc cgatggccgg gagcttcctc gacgaacgcg
     7081 ggacgctgat gggcgccacc gtcgagcccg gcgagccatc gcccgcgggc gaggtcgggt
     7141 cgagcgtctg gtactcctgg acggcgccca cgaccggcga gacggacatc acgttcgaga
     7201 gttggccgca ccccgctcgg atcgcggtgc ataccggcga cgaggtcgac gagctcacgc
     7261 aggtcgccgt ggggggttcg gtccgcttca ggtcgatcga gggcacgacc taccggatcg
     7321 ccgtcgacgg cggcgcggtc gcaagctacc tcgatttcac gatccgcctc acccaccggc
     7381 aatcgcccgc aaacgacgcc ttcgcgaacg ccgagtctct cgcgggcatc gcggccgaga
     7441 cgagcgggac gaccgcaggg gcgacccgcg agccgaacga gcccgaaacc ggactgcgcg
     7501 gggcgaccgt ctggtactcg tggacggcca tgtcggatgg cggtgtgacc gtcgacgtgg
     7561 acacccccga cagcgccgtc ggcgtctaca cgggcgacga cgtggccacg ctcgctcagg
     7621 tcacgcgcgc gcgctcgtcg ttcgacccgc ttcgcgttca gttccgcgcg cgagccggca
     7681 cgacctaccg gatcgcggtc gaccgggcgg atgagtaccg atggggcgcc ccgttcaagc
     7741 tgtcactcaa actgcgtccg ctcccgccga acgacgcctt cgcgctggca actccactcg
     7801 cgccggcacc ggcggcgagc gcctccggca ccacggtcgc cgcgaccgcc gaggcgggtg
     7861 aaccggcggg gggtgacggc cagtcggtgt ggtactcgtg gaccgcgccg gccggtggat
     7921 cgctgacgct cgaggcgacg ggggaccgcc cggtgaagat cgccgcctac accggctcgt
     7981 cggtctccgc gctcacgccg atgccggtcg aggaattgcg cggcggcggg tggacccgcg
     8041 tccgggtcga ggccggcgtc acctaccgga tcgcggtgtg gacgacgcgc accccggcgg
     8101 acttcgacct ctccctgcgg ctcgctcagc gccccgcgaa cgacgacttc gcggaggcgg
     8161 cggtgctctc gggtagtgag gcaggcacgt ccggcaccac ggtcgacgcc acgcgcgagc
     8221 gatgcgacta tccgtcggag accgtctggt accggtggac ggcgccccac accggcgggg
     8281 tgatcctcga cacagccggc agcgagacgc cggtccgagt ggacgtgcac acgggcgact
     8341 cggcctgctc gctcgaggac gcgtcgccag cattgctgag cgatgggggc acgaccgagc
     8401 gggtcagctt ccgcgccgtc gccagcacca cctatcgcat cgccgtgcac ccgttgtggg
     8461 accgcaccgg tccgttccaa ctcgacctgc gactccacgc gccgcctccg aacgacgcgt
     8521 ttgcggacgc gatcgagctg accggttcca gcgatgccgt taccggcacg acgttcgggg
     8581 ccacggagga gctcggcgag gagagtcgcg cggccagcgt ctggtacacg tggaccgcgc
     8641 cctccggcgg gctggccgag gtcggcttcc cacccgccgg gcgcgttccg ggctggaacg
     8701 tgcgcgtata caccggcgac gcgcccggcg cgctgacgca cgtcgcgacg agcaactcga
     8761 acggcctcgc tcacttcaac gcgatggcgg ggcgcactta tcggatcgcg gtgaggggct
     8821 tcggcgggac catcctggag ccgttcaggc tctacctcgc gacggccggc gccccgccga
     8881 acgacgggtt cgccgcggcc gagcgcatcg acggcgactc tggagaggtg cgcgccagca
     8941 cgctcggtgc gggcagcgac ccgggcgagc cggaggggcc gggctggcgc cagcgcgcgt
     9001 ccgtctggta cctgtggacc ccggcacgat ccggcctggc gaagctggag ctgccttcgg
     9061 aggacagggc cgtgacgggg gtgtaccgcg actccccgat gaaccagctc agcctcgtgg
     9121 cctccggcgg ccggaccgtt gggttgcggg tcgtcgcggg cacgacctac cggatcggcg
     9181 tctacggccg tcagtcggtg agctacggag cgttcaagct caggtggcgc acgctgcccg
     9241 ccccggcgaa cgacgatttc gcgaacgccg ccgcactctc gggcgagagc gtggaccact
     9301 acggatcgac catcggtgcg gacagcgagc ccggcgagcc gcccccgccg acccagtcgg
     9361 agggcggtga ggcgagcgtc tggcacgcat ggagggcgcc ggcgtcgggc ctcgtctcgg
     9421 tgaggacgac gagcggggcc gccgacgtgt tcacgggaag cgacatgggc tcgctccaaa
     9481 ggctgaccgt cgcgcccgac tgggccgaga ccgcgtactt ccgcgccgtc gccggcacga
     9541 cctattggat cagggtgagc aacgacctca ccgcgacgac ctacggggac ttccgcctgc
     9601 gactcgccct cgcccagccg gcgccgaacg acgacttcga gcgggctcag cagctcgtgg
     9661 accgggcggt cgggaccacc gccggagccg gcgaccaacc gggcgagccg ctgcgcgacc
     9721 aggagagccg agccaccgtc tggtacacgt ggacgccgga aacgacgggc aagggccgac
     9781 tggcctttgc gcggtcgagc cggggcctga gcgtgtacac gggtgcgcgc ctcgacacgc
     9841 tgcggcgcgt gacgtcagcg agccacggcg acgcggcgga gttccgggcg atcgcaggca
     9901 cgacctacca cgtcgcggtg gacgactggc tcggcattgc gggcgggttc gtcctggacg
     9961 cggccgtgct tccagggccg ccgaacgacg atctcgcgca ggcgaccgtg ctgaccggtg
    10021 gcagtgccag cgcgacgggt cctctcgcga acgcgacgct cgaaccgggt gaggcctcga
    10081 ccaccgacaa ctacgcggag tcctcgacct ggtaccgctg gacggcgcct agcaacggac
    10141 gggtgacctt cgaggcgagc cgacgcacca tcctcgccgc caccggaacg tcgatcggcg
    10201 acctgaagcg cctcgcgcag tcgagcggcc gggtctcggt gccggtgcgc gcgggcacga
    10261 cctacctgtt cggtctctac ggcagcgccg agcaggaggg ggacgtcgag gtcgcgctct
    10321 cgcactctcc cgcgcccgcg aacgacgagt tcgcgaacgc ggaggccctg caggactggc
    10381 cggcgcgggc gagcgggtca aaccaatcgg ccacgcgtga gccgggcgag ccccgcagca
    10441 ccggcggcga cgacgaccgc agcgggacgg tctggtactc gtggaccgcc ccgcagaacg
    10501 gctgggcgga gctcgacctg acgggcaccg ccttcgcccc caccgcaggc gtctacaccg
    10561 gcgacacact cgccgggctg tcgaaggtcg ccgccgcatc ggcgggcgcg ggcgaagcgc
    10621 cgccgaagct gcgctggcga atcgccgccg gggtcacgta ccggatcgcg atcggggggg
    10681 tccgcggcgc ggccggctcg atcgagctgg cgatccggaa ggtcgagacc agaccgaacg
    10741 acgacttcgc cgacgccgag gtcctgtccg gccggtcggc gcgcgtgccg ggctccaacg
    10801 tgggagcgac gaccgagccc ggtgagcaag ggcgctacgg cccgggctac tccgtctggt
    10861 accgctggac ctcggaggtg acggggaacg tcaccgtgca cgccgcgaat accgatgccc
    10921 gcctctggct cgaagccttc accggcaccg aacttcagag tctgagcagc gtcagcgcgg
    10981 gctcccagcg agtcagcttc tacgccgtcg ccggcaccac gtacacggtg cgggtcgcgg
    11041 cagacatcta tgtcccgatg accggttcct acgagctgcg gctcgaccag gtggcagcgc
    11101 ccgcgaacga cgacttcgcc gcgccttcga cactcgaggg cgcctcctcc accacgaccg
    11161 ggaccaccgt gggcgccacg ccgcagggct gcgacccgat cgagttgggc aacggaagcg
    11221 cgccttcggt ctggtactcg tggacggccc ccgcaagcgg cgcggtgact ctgacgaccg
    11281 gagacggggt gtcgccgccg ttcctcggcg tctacaccgg aaccgagcac tgctggctct
    11341 cggcggtccc gacgagccgg ccgaacgcct cgaccgtcac ctttgctgcg caggccggcg
    11401 ttcgctaccg catcatcgtg tccgcccgcc gcggcgcgac cggtccgttc gcgctcgcgc
    11461 tgagcgtcgc gccgtctccg ccgccgcctc ctccggacga gccgcgcaac ccgcccggcg
    11521 atccgccagc gccgcccgat tccgggtcgg gcgaccagcc aagcccgccc gatcccggcc
    11581 ccgccgatcc gccaagcgcg cccgatcccg gccccctggc tcccgccgac ccgccggcgg
    11641 attcgcgccc gagcccagag cggtccgccc cgaccgagcc gcggacctct cctgcggagc
    11701 cgtcgccggg cggcgatccg gcgccggcgg
//
LOCUS       DASVPL010000297        18336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_151145, whole genome shotgun sequence.
ACCESSION   DASVPL010000297 DASVPL010000000
VERSION     DASVPL010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18336
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151145"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VF517_16690"
     CDS             complement(<1..413)
                     /locus_tag="VF517_16690"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054626.1"
                     /translation="MSAIYGLVRLDGRPVEAGELETMRGPMAYWGPDGGGVWREGGAG
                     LGQLVAFRTPADEHETGPLRLPSGTIVSPAGRLDYRDELCAELGVPEEERARTSDGRL
                     IALAYERWGERAFRHLLGDWALAAWHPRERRLVLAR"
     gene            complement(410..850)
                     /locus_tag="VF517_16695"
     CDS             complement(410..850)
                     /locus_tag="VF517_16695"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX8054627.1"
                     /translation="MRRGWSSRARRLLSRDGARVAEAVAYLALARVAVVVLPFRVLAR
                     RLGVRHAETPASATDNPGLGGIAWAIGAAARRAPWRSECLEQAIAAKAMLRRRGVAST
                     LYLGMARDPVGAHAWVRVGDVNVTGGRDVARYAVVASFADVDGR"
     gene            complement(822..1097)
                     /locus_tag="VF517_16700"
     CDS             complement(822..1097)
                     /locus_tag="VF517_16700"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX8054628.1"
                     /translation="MIDRSTVLRRTDDHVSAPLNEWLVMMDIDAGKYYMFDDIASFVW
                     TRLAAPTSVADLVDELCSHYDVPPARCEADILPFLIELDEKGLVQQG"
     gene            1203..2063
                     /locus_tag="VF517_16705"
     CDS             1203..2063
                     /locus_tag="VF517_16705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054629.1"
                     /translation="MEGKAIDAGEFRAGQRRSWNSAASGWEKWMSFTEAAAAPVSRRL
                     VELAGVAPGHRVLDVACGYGEPSFTAARAVAPGGHVVATDISAEMLAAGRERAEREGV
                     ANVEFVESAAAALDLEPQSFDAAVSRWGLIFEPDGEAVATHLRAFLKPGARFAISSWG
                     SPERVPWIGLPMRTVLGRLHAPPPPPGAPGPLSRPTVEAIGGLLEGGGFEGVAAEEIE
                     IDFEWESPDQFTEFCHDIIAPLRMLIDQHPEDVQRDAWAAVTDAAREVAGGDRPLTMT
                     NVVLFASGRA"
     gene            2119..3831
                     /locus_tag="VF517_16710"
     CDS             2119..3831
                     /locus_tag="VF517_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054630.1"
                     /translation="MPRRALLLTLALAALAAAPPAGAQTPPEPPANPTPRAHCKPGSK
                     PEPDIQGRVPKEEVDSGRAADGYWCNIRRIGHSGETGGFRVHRYVDKAGHECAYYDTA
                     LLFPTNALNFSLDLTGVAVLDMSDPRNPVRTQTLVTPAMQTPHESVNISARRGVLAAV
                     MGNPSQYPGGVDVYDISQDCRNPVPMAAAFPATPFGHESGMAPDGMTFYPAAPGSSLS
                     AVDISDERVPKRLWTSEEYSSHGLTISEDGRRAYIGSTAHGLIILDTSEIQDRKPDPQ
                     VREVSRLTWSNKTIPQNAIPVTIKGKPYVVEVDEYSKNEDGDGVAAHGDRVGAARIID
                     ISDERKPFVVSNIRLEVHQKQNRPAIAGDYGAQSPVQGYAAHYCNVPKPVDPGIVACS
                     MILSGLRVFDIRDPRNPKEIAYHVAPPSTISATGAPVIDERANWAMSQPDFAPERGEI
                     WYSDGTSGFYALKVDESVWPFKEDAPGGGGCVRQPSAAFKIHRIEGTRVVRVEAFVNG
                     KRRLLQTGRDIRRITLKGLKRSGKLTVRIVATHNTGSKVVSTRSWNGCTKGKPRVRRI
                     PRPR"
     gene            complement(3847..5736)
                     /locus_tag="VF517_16715"
     CDS             complement(3847..5736)
                     /locus_tag="VF517_16715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8054631.1"
                     /translation="MRISRALGSAAAIAAMAACPTAFAASAPDAGRAFKPPPLPQAKY
                     EVSAPTQDNGQIRIVRAADGVDLYTETWLPAPKDGAAPPKRVPIVIQYSPYSTKGSPE
                     NPKRMQLLVSRGYAYTQAHVRGSGGSGGCIEQTAENEVDDGARIVEDAGERAPWSARR
                     VGMYGISYRGGTQIATATGPDRDRLETLRAIVVGAPVASAYEFFAHDGVPHLAQGSAN
                     VLAYSLALSTPWDSPERLPERPGCQPQILAGSLDLSGNYTPHFAARDHTRNLRRLRAA
                     TLMWHGHADTRVSEHGQIGLFDGIPRGVPRRGFFGVWEHEHPDEFRGNTPEPRRDWER
                     MDWDAAVVAWYERYLRGRRNRVESWPVAQVQGTDGQWRTARDWPREPGGSARELLLGP
                     DATLGARNPAGATSYTEAGLPELSLDGYTHPPGTAAVFTTPPLADRLELYGAPELRLW
                     LELDRPDAHVAVKVEALDAAGNRTMPEARTVGARSMQHLDPFDAGRFRQAQGTPAPVG
                     QPFAVTVRLDPTDLVVPRGGRLRVTITGSVQAYDGLDGLAEGAGAFVQGPTAPSGSAT
                     GVRILHSCAHPSALRFTTPDSRSALIDVRERDQETARLGTSPAAPRPPVTGGGLAAKP
                     GRACG"
     gene            complement(5818..7581)
                     /locus_tag="VF517_16720"
     CDS             complement(5818..7581)
                     /locus_tag="VF517_16720"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX8054632.1"
                     /translation="MDSRARLLPLIAAAALAVLVALAVQLTGALDSLERQSLGARFQV
                     RGEQRPDEFVVVGIDDATFDDLGVSWPFRRSLHARAVDRLHEAGARTIVYDVQFTEGT
                     KDSEDMALYEALDRAGGAVLATSESDSHGATNVLGGDENLRAIDSRAAGSDLTNDSTG
                     AVERFPYSVSGLESVGVVAAERASGRPVDPELFEDGQARIDFRGGPGTVRTVSFSDLL
                     RGRVDPAVLRGKIVVVGATAPTLRDVHNTPVHGDELMSGPEAQANAIWTALHGVPLHD
                     APPFLDYAAILLLGVLAPLARMRWRILAAAGAGIGAAAAYVVANQLAFESGTVLAVTA
                     PITAALVGTGGMTVVSHLRETLERRRVARELRVSQLEVIHRLSAAVESRDEETGLHVE
                     RISWFAQRLALRSGSNAAEAELIGRASALHDVGKISIPDHVLLKPGKLDPAEWELMKS
                     HTTAGAAILAGSSSPFVQMAETIARTHHERWDGSGYPAGLAGEEIPLAGRICGICDVF
                     DALLSRRPYKDPWRLDRALAEIERLSGSHFDPRLVVDFLEIARELHGEWRYEPEEAIS
                     FDAPMDFVSWRAADAENSKLP"
     gene            complement(7597..8856)
                     /locus_tag="VF517_16725"
     CDS             complement(7597..8856)
                     /locus_tag="VF517_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054633.1"
                     /translation="MRPFLTLASAALLTAVVGVSSASAGFLDLTGVSLVKTDPAAAGL
                     PGAETRPASNIGDDTARMNGKVDPHERPTTYHFDWGQTSGYGSRTTDTAAGRGDSGVS
                     AAATIGGLRPGTTYHFRIVAENDAGTTRGADRTFTTTGAAAPDDPAGTPGDPGTGASD
                     PGTGASDPGTAAGDPGSSPDGTPGDPDGGASTPGDGTTPQGDGALGSVPPDTSPGAEQ
                     DAADPFVIGGQAKKHREFAVAPTAGHISVNPPGPTGFQPLVEGVSVPVGSIVDARNGT
                     AAITTELDSGRKQTAEFWGERFKVRQPVEEGGLTEIRVRDAVRACDGKSISAVDKVVS
                     ARKKRRRGGLWGRDAKGRWRTHGRGSQATTRGTIWFSEERCDGTYTKVVKGSVLVRDH
                     YLKRNVVVNAGGTYLAHPHRKHKPSLR"
     gene            8969..10741
                     /locus_tag="VF517_16730"
     CDS             8969..10741
                     /locus_tag="VF517_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054634.1"
                     /translation="MPPAHSLRRLLAAFLVCLASIAGAGCLGGGDDGKDAKKPPIGAR
                     GDFQLKKSLVPKGDSAAAEGAPESYVPEGEIVADSGFRPEVDGFAFENYGNEGDPRNL
                     GPAEMEALFGDQVCLSGTGDDCQLIPTAQEWMDNQNEGMAGGHCEGFSIAALRMFSEA
                     LDQEDFGGDTTAGLDIVGNDALQGSIAEHFVYQVLPPIVENRVSGTPSEVLDKLTEAL
                     DSGEEQYTLGVYKADGTAGHAITPFAIEDKGDGQFAILVYDNNFPGLTRAVEVDTNDD
                     SWSYVSGTNPDDLGQVYQGDADTQTLELDPTTPGEEQQPCPFCDETAGDGGEETKGSL
                     LPKDERYTEITLSGDPRNHPHLVFEDDDGNRTGIVDGRMLSEIEDVEVVKTFATQNWE
                     GGPEPRYRLPEGADYTISVDGTDLDAPAKIDIDLVGSGLVIEIEDVKIAPGQTDAMAL
                     PGGYGITYGTDSKEEEAPNLYASLVEDDYAYTFAATAVGIKRGSTLSLVVEQENKVAY
                     IDSTGGKGADGGTGVYILNLTRADAEGNIAQWQNGEVMLDGSKDEKAGFEYDESPEPG
                     KPLPLVLLDANGDPTDTIEMQPQK"
     gene            10797..11807
                     /locus_tag="VF517_16735"
     CDS             10797..11807
                     /locus_tag="VF517_16735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX8054635.1"
                     /translation="MACVAAGLCFPSGAAALPAKGTRVTIAATGDLLIHSPVWQRALS
                     YGGGRRYDFRPMFRKIGPLIAGADLALCHVETPLTEGAPHGYPSFRTPAALATAVRAT
                     GWDVCSTASNHTLDGGQRGVNSTLDILDKAGVRHAGSYRTRSAAREPTILEAHGVRVA
                     FLSYTALTNGQRVPHEWSVNMARAPRILKDAKRARQAGADAVVVNVHWGDEFRSGVTR
                     SQRSLAATLTRSKYVTAVVGQHVHVVQPIRWVNGKPVVFGEGNLISNQTAACCPAASQ
                     DGLIALLDLRVGAAGVRAKRVRYLPTYVRHPDYTVVRAGGRSRARTIRVAGRSKRVVP
                     IR"
     gene            11897..12520
                     /locus_tag="VF517_16740"
     CDS             11897..12520
                     /locus_tag="VF517_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8054636.1"
                     /translation="MEVALDSVHPKPPRSETAERLLDAAERLLVEVGHGGITTRRLAA
                     EAGANQGLVHYYFGSIDELFVQVLERFTERLIARQREMYGTDAPFIEKWRTAWRFQEE
                     DLRGGYSKIWLELQARSWSRPELRPRVARVDAEWRAVLREAYRRAAAEYGLDEEEFPV
                     EALVALTMTFGQGFALERLEGIDEGHAELLGWIERWIESLEDRRGSR"
     gene            12517..14688
                     /locus_tag="VF517_16745"
     CDS             12517..14688
                     /locus_tag="VF517_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8054637.1"
                     /translation="MTVTAPDEVRCRDEQSRARYPDETGFVERDGVRVFWESYGQGEQ
                     TVLFLPTWTLNHSRIWKAQIPYFARHFRVLCFDPRGNGRSDRPTDSAAYAEHEFAQDA
                     IDVMDACGVERAVAVGLSRGAQRGLLLAAEHPERVTGLALIAPLFPVSRVRSVQQRMA
                     FHPRAAAFWKRRPPTTRGQGKFSPYYWTHGGYADFVQWWAERMLTEPHSTKQIEDAVA
                     WSHETDGDCLAASLTAPLAAPATRRDQIALAERVRCPVLVIHGTKDRVMPYPDGKALA
                     GVTGGRLETVEAAGHLPHARKPVQVNLALREFVEGAPLRDPTVHRADGRKRALYISSP
                     IGLGHAQRDIAIARELRKLVDGLEIDWLAQDPVTRVLEREGETIHAASDHLANESQHI
                     ESESAEHDLHCFHAWRRMDEILINNFMVFHDVVREEKYDLWVGDEAWELDYYLHENPR
                     EKRARYAWLTDFVGWLPMPDGGEREAFLTADYNAEMIEHIEGHPDLRDRAIFVGNPDD
                     IVDERLGPQLPMIRDWTERNFEFTGYVTGFDPSTLGDRDELRHELGYRPDEKVCIVSV
                     GGSGVGGHLLRRVIASFDAAKRQVPELRMIVVAGPRIDPDSLPQHDGLEVVAYVHNLY
                     RHLAACDLAVVQGGLTTGMELTASGRPFLYFPLRHHFEQNMHVAHRLDRYGAGRRMDF
                     DTADPDVIAGAIAAEIGRRVEYRPVETDGARRAAEKLAELL"
     gene            14872..15090
                     /locus_tag="VF517_16750"
     CDS             14872..15090
                     /locus_tag="VF517_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054638.1"
                     /translation="MKPIAGTDSCEAPHFQYHVSGQLAIRMDDGTEFVAKAGDITSLP
                     SGHDAWVVGEEPVVVVDWYGASSYAKGG"
     gene            complement(15109..15744)
                     /locus_tag="VF517_16755"
     CDS             complement(15109..15744)
                     /locus_tag="VF517_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054639.1"
                     /translation="MRRCSIHLCAVLAAVAYAITAPAPAAAAGNSCRAESLKSGNTVT
                     VANRTGVIFESRRLQQTVACTYKHRRLVKLRGVCCQFARFQLGSRYLGFSYRLDEAFN
                     EVDEMGVFDLKTGKRVPFSKQSGPATVDTGGYVESFQVTAKGGLVWVQGFKNGDDEPD
                     PNDVAVRTIARGETPQTLDRGKIDPDSFAVSSGGKTVYWSNDGTVKSAPIG"
     gene            complement(15851..16246)
                     /locus_tag="VF517_16760"
     CDS             complement(15851..16246)
                     /locus_tag="VF517_16760"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8054640.1"
                     /translation="MEITRNSLETATGPSDWFTGTVHIDQVAAAPAPSRLNAAMVHFT
                     PGARTAWHTHPLGQTIYVTEGVGRCQRRGGPVETIRPGDRVFFEPDEDHWHGAAPDRL
                     MTHLAMQQADESGSPVTWGEHVTDAEYGQ"
     gene            complement(16252..17289)
                     /locus_tag="VF517_16765"
     CDS             complement(16252..17289)
                     /locus_tag="VF517_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX8054641.1"
                     /translation="MRAAIFNETRSITVGDRPDASLVEPTDAVVRVVLACVCGSDLWY
                     YRGDTPFEPGPIGHEFVGVVEDVGSSVQNVSKGDFVIAPFAYSDGTCPHCQHGITTAC
                     SNGGFFPMNGDGGQGEAVRVPLADGTLVTVPGSGHSEEMLASLLTLSDVMATGHHAAV
                     CARVKEGDTVAVVGDGAVGLSAVLAANRLGAERVIALSRHEKRQELAREFGAADVVEQ
                     RGREATEAVMELTQGIGVDAALECVGTGQAMKTALSIARPGSVVGYVGVPHGVEFSVD
                     QMFYRNKGVLGGPAPARAYIPELLGDVLEGRIDPGRVFDYETGLDGIADAYAAMDERR
                     AIKSLVRVGTV"
     gene            17330..17986
                     /locus_tag="VF517_16770"
     CDS             17330..17986
                     /locus_tag="VF517_16770"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054642.1"
                     /translation="MSSPLADHGTCRPLRRDAERNRQRILTAAAEVFAARGLDVTLDD
                     IAAHAGVGVGTVYRRFANKDELIDAVFGEQLDEVAAIVSDALEAPDAWTGLTTYLERS
                     LRMQLEDRGFTEIFYGRSGRRLDVARERLAPLTEMVVDRARREGAVREDLERTDLVFV
                     QLALAAMMDATRGAEPELYRRYLALFLDGLRPAREGPSALPVPALSGEQTHAVMTRGG
                     "
     gene            18119..18292
                     /locus_tag="VF517_16775"
     CDS             18119..18292
                     /locus_tag="VF517_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054643.1"
                     /translation="MSDSEAVRTALREAAARRRARSAVAEEVQRLADDVADREEMRLI
                     REQMSELAPDLPD"
BASE COUNT         2669 a         6500 c         6629 g         2538 t
ORIGIN      
        1 cgggccagga ccagccgccg ctcgcgcggg tgccaggccg cgagcgccca gtcgcccagc
       61 aggtgccgga atgcccgctc gccccagcgc tcgtacgcca gggcgatgag ccggccgtcg
      121 gacgtgcgcg cccgctcctc ctccggcacc ccgagctcgg cgcacagctc gtcgcggtag
      181 tcgagccgcc cggcggggct cacgatcgtt ccactcggga gccgcagcgg gccggtctcg
      241 tgctcgtccg ccggcgtgcg gaaggcgacg agctgcccga ggcccgctcc gccctcgcgc
      301 cagacccctc cgccgtcggg accccagtac gccatcggcc cccgcatcgt ctcgagctcg
      361 ccggcctcga cgggacggcc gtcgaggcgc acgaggccgt agatcgcgct catcgaccgt
      421 cgacgtctgc gaacgaggcc acgaccgcgt accgggcgac gtcacgcccg cccgtcacgt
      481 tcacgtcgcc cacgcgcacc caggcatgag cccccaccgg gtcgcgcgcc atgccgagat
      541 agagcgtgct ggcgacaccg cgccgccgca gcatcgcctt cgccgcgatc gcctgctcga
      601 ggcattccga ccgccagggc gcccgccgcg ccgcggcgcc gatcgcccat gcgatcccgc
      661 ccagaccggg gttatcggtc gcgctcgccg gtgtctcggc gtgccggacc ccgagtcgtc
      721 gcgcgagcac tcggaagggc aggacgacca ccgcgacccg cgcgagcgcc aggtacgcaa
      781 ccgcctcggc gactcgcgcg ccgtcgcgcg acaggagccg cctagccctg ctggaccagc
      841 cccttctcat cgagctcgat caggaagggc aggatgtcgg cctcgcagcg cgcgggaggg
      901 acgtcgtagt gggagcacag ctcgtcgacg aggtcggcga cggacgtcgg cgcggccagt
      961 cgcgtccaga cgaaggaggc gatgtcgtcg aacatgtagt acttccccgc gtcgatgtcc
     1021 atcatcacga gccactcgtt gaggggcgca ctcacgtggt cgtcggtacg gcgcaggacg
     1081 gtggaccgat cgatcatgcg cgtaggttag gtgcggccgg cgattgcaga cagcggtagg
     1141 gtcgggcttg cgcgggcgcc gtatctcggc gctcgtggtt cacggcgact gaggaggcag
     1201 cggtggaggg caaggccatc gacgccggtg agttccgcgc cggacagagg cgctcctgga
     1261 actcggccgc gagcggctgg gagaagtgga tgtcgttcac cgaggccgcg gcggccccgg
     1321 tcagccgccg gctcgtggaa ctggcgggcg tcgcccccgg ccaccgcgtc ctggacgtcg
     1381 cgtgcggcta cggtgagccg tcgttcaccg cggcccgcgc ggttgccccg ggcgggcacg
     1441 tggtcgcgac cgacatctcg gccgagatgc tcgccgccgg ccgcgagcgc gcggagcgag
     1501 agggcgtcgc caacgtcgag ttcgtcgagt cggccgcggc ggctctcgac ctcgagccgc
     1561 agagcttcga cgccgccgtc tcgcgctggg gcctgatctt cgagcccgac ggcgaggcgg
     1621 tggcgacgca cctgcgcgcg ttcctgaagc cgggcgcgcg cttcgcgatc tcgtcctggg
     1681 gatcgccgga gcgggtgccg tggatcggtc tgccgatgcg caccgtgctg ggccggctgc
     1741 acgcgccgcc gccgcccccc ggggcgccgg gcccgctgtc gcgcccgacg gtggaggcca
     1801 tcggcggcct gctcgagggc gggggcttcg agggcgtcgc ggccgaggag atcgagatcg
     1861 acttcgagtg ggagtcgccc gaccagttca ccgagttctg ccacgacatc atcgccccgc
     1921 tccggatgct gatcgatcag catccggagg acgtccagcg ggacgcctgg gcggcggtca
     1981 ccgacgccgc gcgggaggtc gccggcggcg atcgcccgct gaccatgacg aacgtggtgc
     2041 tgttcgcgtc gggtcgcgcc tagcccgcgc tggggtgcag cacgagcgcg gattttggtg
     2101 ccacactcgc gagaaccgat gcctcgccgc gccctgctcc tgaccctcgc cctcgccgcg
     2161 ctcgccgcgg ccccgcccgc cggcgcgcag acgccgcccg agccgcccgc gaacccgacc
     2221 ccgcgcgccc actgcaagcc cggctcgaag cccgagcccg acatccaggg gcgcgtgccc
     2281 aaggaggagg tcgactccgg gcgggccgcc gacggctact ggtgcaacat caggcgcatc
     2341 ggtcacagcg gcgagaccgg cgggttccgc gtgcatcgct acgtggacaa ggccggccac
     2401 gagtgcgcct actacgacac cgcgctgctg ttcccgacca acgccctcaa cttcagcctc
     2461 gacctcaccg gcgtcgccgt gctcgacatg tcggacccgc gcaacccggt gcggacgcag
     2521 acgctcgtga ccccggccat gcagaccccg cacgagtcgg tgaacatcag cgcgaggcgc
     2581 ggcgtgctcg ctgccgtgat gggcaacccc tcgcagtacc cgggcggcgt cgacgtctac
     2641 gacatcagcc aggactgccg caacccggtg ccgatggcgg cggcgtttcc ggcgacgccg
     2701 ttcggccacg agagcggcat ggcgccggac ggtatgacgt tctacccggc cgcgcccggc
     2761 tcgagcctct cagccgtcga catctccgac gagcgcgtgc cgaagcgcct gtggacgagc
     2821 gaggagtact cgtcgcacgg gctgacgatc tccgaggacg gccggcgtgc atacatcggc
     2881 tcgaccgcgc acggcctgat catcctcgac acctccgaga tccaggaccg caagccggac
     2941 ccgcaggtgc gcgaggtgag ccgtctgacg tggtcgaaca agacgatccc gcagaacgcg
     3001 atcccggtga ccatcaaggg caagccgtac gtggtggagg tcgacgagta ctcgaagaac
     3061 gaggacggcg acggcgtggc ggctcatggc gaccgcgtcg gcgccgcgcg gatcatcgac
     3121 atctcggacg agcgcaagcc gttcgtagtg tcgaacatcc gcctcgaggt ccaccagaag
     3181 cagaaccggc cggcgatagc gggcgactac ggcgcgcaga gccccgtcca gggctacgcc
     3241 gcgcactact gcaacgtgcc gaagcccgtc gaccccggca tcgtggcctg ctcgatgatc
     3301 ctctcggggc tgcgcgtgtt cgacatccgc gaccccagga atccgaagga gatcgcctac
     3361 cacgtggcgc cgccgtcgac catcagcgcg accggcgccc ccgtgatcga cgagcgtgcg
     3421 aactgggcga tgtcacagcc ggatttcgcg cccgagcgcg gggagatctg gtactcggac
     3481 gggacgagcg gcttctacgc gctgaaggtc gacgagagcg tctggccgtt caaggaggac
     3541 gcccccggag gcggcggctg cgtgcgccag ccgtcggccg cgttcaagat ccaccgcatc
     3601 gaggggacgc gcgtcgtacg ggtcgaggcg ttcgtgaacg gcaagcgccg cctgctccag
     3661 accggtcgcg acatcaggcg catcaccctg aaggggctga agcgcagcgg caagctgacc
     3721 gtccggatcg tcgcgaccca caacaccggc tcgaaggtcg tgagcacgcg cagctggaac
     3781 ggctgcacca agggcaagcc gcgcgtgcgc cggatcccgc ggccgcgctg acgctctcgg
     3841 cgcggctcag ccgcaggcgc ggccgggctt agccgccagg ccgccgccgg tcaccggcgg
     3901 gcgcggcgct gcgggcgagg tccccagcct cgccgtctcc tgatcgcgct cccggacgtc
     3961 gatcagcgcg gacctcgagt cgggcgtggt gaagcgcagc gcgctcgggt gcgcgcacga
     4021 atgcaggatc ctcacgcccg tcgcgctgcc cgacggagcg gtcgggccct ggacgaaggc
     4081 gcctgcgcct tcggcgaggc cgtcgagacc gtcgtacgcc tggaccgagc cggtgatcgt
     4141 cacgcgcagt cgcccgccgc gcgggaccac gagatcggtc gggtcgaggc ggaccgtgac
     4201 ggcgaacggc tggccgaccg gcgccggcgt gccctgcgcc tgccggaagc gaccggcgtc
     4261 gaacgggtcg aggtgctgca tcgaccgcgc gccgacggtg cgcgcctcgg gcatcgtgcg
     4321 attgcccgcc gcgtcgagcg cctcgacttt gaccgccaca tgcgcgtcgg ggcggtcgag
     4381 ctcgagccac aggcgcagct ccggcgcgcc gtagagctcg agccggtcgg cgagcggcgg
     4441 agtggtgaac accgctgccg tgccgggagg atgggtgtag ccgtcgagcg agagctccgg
     4501 cagcccggcc tccgtgtacg acgttgcgcc ggccgggttc ctggccccga gcgtggcgtc
     4561 cggtccgagc agcaactcgc gagcggaccc gcccggctcg cgcggccagt cgcgtgccgt
     4621 ccgccactgg ccgtcggtcc cctgcacctg cgcgaccggc cacgactcca cacggttgcg
     4681 gcgcccgcgc aagtagcgct cgtaccacgc gacgacggcc gcgtcccagt ccatccgctc
     4741 ccagtcccgg cgcggctcgg gcgtgttccc gcggaactcg tccgggtgct cgtgctccca
     4801 cacgccgaag aagccgcgcc tgggaacgcc gcgcggaatg ccgtcgaaca acccgatctg
     4861 gccgtgctcg gagacgcggg tgtccgcgtg gccgtgccac atcagggtgg cggcgcggag
     4921 ccgccggagg ttgcgcgtgt ggtcgcgtgc cgcgaagtgc ggcgtgtagt tcccgctcag
     4981 gtccagcgag cccgccagga tctgcggctg gcagcccggc cgctcgggga gccgctcggg
     5041 cgaatcccac ggagtcgaca gcgcgagcga gtaggcgagc acgttggcgg agccctgcgc
     5101 caaatgcggg acgccgtcgt gcgcgaagaa ctcgtacgcg ctcgcgaccg gcgcgccgac
     5161 gacgatcgcc ctgagcgtct cgagccggtc gcggtcgggg cccgtcgccg tcgcgatctg
     5221 cgtgccgccc cggtaggaga tgccgtacat cccgacgcgc cgggccgacc acggcgcccg
     5281 ctcgccggcg tcctcgacga tgcgcgcgcc gtcgtcgacc tcgttctcgg cggtctgctc
     5341 gatgcagcca ccggagccgc ccgacccgcg gacgtgcgcc tgcgtgtagg cgtagccgcg
     5401 gctcacgagc agctgcatgc gtttcgggtt ctcgggcgag cccttggtgg agtacggcga
     5461 gtactggatc acgatcggaa cgcgcttcgg cggcgccgcg ccgtccttgg gcgcggggag
     5521 ccacgtctcg gtgtagaggt caacgccgtc ggcggctcgc acgatccgga tctgcccgtt
     5581 gtcctgggtg ggagcggaaa cctcgtactt cgcctgcggc agcggcggcg gcttgaacgc
     5641 ccgcccggcg tccggcgcgc tcgcggcgaa cgcggtgggg cacgccgcca ttgcggcgat
     5701 ggcggcggcg gaaccgagcg cgcgcgagat cctcacgcgg tggaacgtac ctcgccggcg
     5761 gtcatctcac acgcctcatg cggcccgcgc ggcggaaggg ccaggccgat cgggcgacta
     5821 cggaagcttc gaattctcgg cgtcggccgc ccgccagctc acgaagtcca tcggggcgtc
     5881 gaaactgatc gcctcctcgg gctcgtatcg ccactcgccg tggagctcgc gcgcgatctc
     5941 gaggaagtcc actaccagcc gcgggtcgaa gtggcttccg ctcaggcgct cgatctccgc
     6001 gagcgctcgg tcgagccgcc acgggtcctt gtagggccgc ctcgagagca acgcgtcgaa
     6061 cacgtcgcat atcccgcaga tccgcccggc cagcgggatc tcctcgccgg cgagtcccgc
     6121 cggatatccg ctgccgtccc agcgctcgtg gtgggtgcgc gcgatcgtct cggccatctg
     6181 cacgaacggc gagctcgatc cggcgagaat tgccgcaccg gcggtggtgt gcgacttcat
     6241 cagctcccac tcggccgggt ccagcttgcc cggcttgagc aggacatggt cgggaatgga
     6301 gatctttccg acgtcgtgca gcgcgctcgc gcggccgatc agctcggcct ccgcggcgtt
     6361 cgacccgctc ctgagcgcaa gccgctgtgc gaaccagctg atccgctcga cgtggagtcc
     6421 cgtctcctcg tcgcgtgact cgacggccgc gctgagccgg tggatcacct cgagctggct
     6481 gacgcgcagc tcacgagcca cgcgccggcg ctcgagggtc tcacgcaggt ggctcacgac
     6541 cgtcattcca ccggttccca cgagcgcggc cgtgatcggc gccgtgacgg cgaggacggt
     6601 gccgctctcg aaggccagct ggttggcgac gacgtaggca gcagccgcgc cgatgccggc
     6661 gccggcggcg gccaggatcc gccagcgcat ccgcgcgagc ggcgccagca cgccgagcag
     6721 gaggatcgcg gcgtagtcga ggaacggcgg cgcgtcgtgc agcggcacgc cgtgcagcgc
     6781 ggtccagatc gcgtttgcct gagcctccgg acccgacatc agctcgtcgc cgtggaccgg
     6841 cgtgttgtgg acgtcgcgga gcgtgggcgc cgtggcgccg accaccacga tcttcccgcg
     6901 cagcaccgcc gggtcgaccc tgcctcgcag caggtcggag aacgagaccg tgcggacggt
     6961 gcccgggcca ccgcggaagt cgatccgggc ctgcccgtcc tcgaagagct ctgggtccac
     7021 cggacggccg ctcgcgcgct ccgcggcgac cacgcccacg ctctcgaggc cactgacgga
     7081 gtaggggaag cgctcgaccg ctccggtcga gtcgttcgtc aggtcgctgc ccgccgcgcg
     7141 cgagtcgatc gctcgcaggt tctcgtcgcc gccgagcacg ttggtggcgc cgtggctgtc
     7201 gctctcgctg gtggccagca cggctccgcc ggcgcggtcg agagcttcat agagggccat
     7261 gtcctcagag tccttggtcc cttcggtgaa ctgcacgtcg tagacgatcg tgcgcgcccc
     7321 ggcctcgtga agccggtcga ccgcgcgcgc gtgaagcgag cgcctgaagg gccaagagac
     7381 gccgaggtcg tcgaaggtcg cgtcgtcgat ccccaccacc acgaactcat cggggcgctg
     7441 ctcgccacgt acctgaaagc gcgcgccgag cgactggcgc tcgagcgagt cgagcgcgcc
     7501 ggtgagctgg accgcgaggg ccacgagcac cgccagggcc gccgcggcga tcaggggcag
     7561 cagcctggct cgagagtcca tcgcggagcc ctgtccctag cggaggctgg gcttgtgctt
     7621 gcggtggggg tgagcgaggt acgtcccgcc ggcgttcacc acgacgttgc gcttgaggta
     7681 gtggtcgcgc accaggaccg agcccttgac gaccttggtg taggtgccgt cgcagcgctc
     7741 ctcgctgaac cagatcgtcc cgcgcgtggt ggcctggctg ccgcgcccgt gggtgcgcca
     7801 gcggcccttg gcgtcccggc cccagaggcc gccgcggcgg cgcttcttgc gggcggagac
     7861 gaccttgtcg accgcggaga tggacttgcc gtcgcaggcc cgcacggcgt cgcggaccct
     7921 gatctcggtg aggccgcctt cctcgacggg ctgacgcacc ttgaagcgct cgccccagaa
     7981 ctcggcggtc tgcttgcggc ccgagtcgag ctcggtggtg atcgcggcgg tgccgttgcg
     8041 tgcgtcgacg atgctgccga ccggcacgct cacgccctcg accagcggct ggaagccggt
     8101 ggggccgggc gggttgacgc tgatgtgccc ggcggtgggg gcgacggcga attccctgtg
     8161 cttcttggcc tggccaccga tcacgaacgg gtcggcggcg tcctgctcgg caccggggct
     8221 cgtgtccggg gggacgctgc cgagggcgcc gtcgccctgc ggcgtcgtgc cgtcaccggg
     8281 ggtcgatgcg ccgccatcgg gatcgccggg ggtgccgtcg gggctcgatc cggggtcgcc
     8341 cgcagccgtg ccggggtcgc ttgcacccgt accggggtcg ctcgcacccg taccggggtc
     8401 gccgggcgtg cctgccggat cgtcgggcgc ggccgcgccg gtggtggtga aggtcctgtc
     8461 ggctccacgg gtggtgccgg cgtcgttctc ggcgacgatg cggaagtggt acgtggtgcc
     8521 cggccggaga ccgccgatcg tggccgcggc ggacacgccg ctgtcgcccc ggcccgccgc
     8581 ggtgtccgtg gtcctggacc cgtagccgct cgtctggccc cagtcgaagt ggtaggtcgt
     8641 gggccgctcg tgcggatcga ccttgccgtt catccgtgcg gtgtcgtccc cgatgttcga
     8701 ggccggccgg gtctctgcgc ccgggaggcc tgcggcggca gggtccgtct tcacgagtga
     8761 cacgccggtg aggtcgagga acccggccga cgcggacgac acgcctacca ccgccgtgag
     8821 cagggcggca gaggccaggg tgaggaaggg gcgcacccgg ttcctgtcgg atttccggcg
     8881 gatgacttga tggccctgga cgactgttcg cccacttcgg cgcgccggcg cgtaggaatg
     8941 tccgtcgcgc acgcgaagct cctgcgccat gccaccagcc cacagcctgc gccgcctgct
     9001 cgccgcgttt ctcgtctgcc tagcctcgat cgccggcgcc ggctgcctcg gcggtggcga
     9061 cgacggcaag gacgcgaaga agccgccgat cggcgcccgc ggcgacttcc agctcaagaa
     9121 gagcctcgtc cccaagggcg acagcgccgc cgccgaaggt gctcccgaga gctacgtgcc
     9181 cgagggtgag atcgtggcgg acagcggctt ccggccggag gtcgacggct tcgccttcga
     9241 gaactacggc aacgagggcg acccgcgcaa cctcggtccg gcggagatgg aggcgctctt
     9301 cggcgatcag gtctgcctgt ccggcacggg cgacgactgc cagctcatcc cgaccgccca
     9361 ggagtggatg gacaaccaga acgagggcat ggcaggcggc cactgcgagg gcttctccat
     9421 cgccgccctt cggatgttca gcgaggcgct cgaccaggag gacttcggcg gcgacaccac
     9481 cgccggtctc gacatcgtcg gcaacgacgc gctccagggc tcgatcgcgg agcacttcgt
     9541 ctaccaggtc cttccgccga tcgtggagaa ccgtgtgtcc ggcacgccga gcgaggtgct
     9601 cgacaagctg accgaggcgc tcgacagcgg cgaggagcag tacacgctcg gcgtctacaa
     9661 ggccgacggc accgccggac acgcgatcac gccgttcgcg atcgaggaca agggcgacgg
     9721 ccagttcgcg atcctcgtct acgacaacaa cttccccggg ctcacgcgcg cggtcgaggt
     9781 ggacacgaac gacgactcct ggagctacgt gagcgggacg aaccccgacg acctcggaca
     9841 ggtctaccag ggcgacgccg atacgcagac gctcgagctc gatccgacca cccccggtga
     9901 ggagcagcag ccgtgcccgt tctgcgacga gaccgccggg gacggcggag aggagacgaa
     9961 ggggtcgctg ctgccgaagg acgagcgcta caccgagatc accctgagcg gcgacccgcg
    10021 caaccacccg catctggtgt tcgaggacga cgacggcaac cgcaccggga tcgtcgacgg
    10081 ccgcatgctg agcgagatcg aggacgtcga ggtcgtcaag acgttcgcga cgcagaactg
    10141 ggagggcggt cccgagccgc gctaccgcct ccccgagggc gccgactaca cgatcagcgt
    10201 ggacggcacc gacctcgacg caccggcgaa gatcgacatc gacctcgtcg gcagcgggct
    10261 ggtgatcgag atcgaggacg tcaagatcgc gccgggtcag accgacgcga tggcgctgcc
    10321 cggcggctac gggatcacct acggcaccga cagcaaggag gaggaggcgc cgaacctcta
    10381 cgccagcctg gtcgaggacg actacgcgta caccttcgcc gcgaccgcgg tgggcatcaa
    10441 gcgcggctcg acgctcagcc tggtcgtgga gcaggagaac aaggtcgcct acatcgacag
    10501 caccggcggc aagggagccg acggcggcac cggcgtctac atcctcaacc tcacgcgcgc
    10561 ggacgccgag ggcaacatcg cgcagtggca gaacggcgag gtcatgctcg atgggtcgaa
    10621 ggacgagaag gccgggttcg agtacgacga gtcgccggag ccgggcaagc cgctgccgct
    10681 ggtgctcctc gacgccaacg gcgacccgac cgacacgatc gagatgcagc cgcagaagtg
    10741 accgcggccg cgccggcgcg ccggcccccg ccatgagtcg cgcggcgaga ctgatcatgg
    10801 cgtgcgtcgc cgccgggctc tgcttcccgt ccggcgcggc ggccctgccg gccaagggca
    10861 cgcgcgtgac gatcgccgcc accggcgatc tgctgatcca cagcccggtc tggcagcggg
    10921 cgctctccta cggcggcggc cgccgctacg acttccgccc gatgttccga aagatcgggc
    10981 cgctgatcgc gggcgcggac ctcgcgctct gtcacgtgga gacaccgctc acagaaggcg
    11041 cgccgcacgg ctacccgagc tttcgcaccc ccgcggcgct ggccacggcc gtgcgagcca
    11101 ccgggtggga cgtgtgcagc acggcctcga accacaccct cgacggcggc cagcgcggcg
    11161 tgaacagcac gctggacatc ctcgacaaag cgggtgtgcg gcacgcgggc tcctaccgca
    11221 cgcgctcggc ggcgcgtgag ccgaccatcc tcgaggcgca cggggtcagg gtcgccttcc
    11281 tctcgtacac ggcgctcacc aacggccagc gggtgccgca cgaatggtcg gtcaacatgg
    11341 cccgcgcgcc ccggatcctg aaggacgcca agcgcgctcg gcaggcgggc gccgacgcgg
    11401 tggtcgtcaa cgtccactgg ggcgacgagt tccgcagcgg cgtgacgcga tcgcagcgtt
    11461 cgctcgccgc cacgctcacg cgctcgaagt acgtgaccgc ggtcgtgggc cagcacgtgc
    11521 acgtggtcca gccgatccgc tgggtcaacg gcaagccggt ggtgttcggc gagggcaacc
    11581 tgatctcgaa ccagaccgcg gcctgctgcc cggcggcctc gcaggacggg ctgatcgcgc
    11641 tgctcgacct gcgcgtcggc gcggcgggcg tcagggccaa gcgcgtgcgc tacctgccca
    11701 cgtacgtgcg gcacccggac tacacggtgg tccgcgcggg cggacgctcg cgtgcgcgga
    11761 cgatcagggt ggccgggcgc tcgaaacgcg tcgtcccgat ccgctgatcg gccggagcag
    11821 ccggtgacgg ccggcggccc ttgacaacgc gcgtccgcgc tgttattcat ccgccttagg
    11881 cgatcgccta agtcacatgg aagtcgcact cgacagcgtc caccccaagc cccctcgctc
    11941 cgagacggcc gagcggctgc tcgacgccgc cgagcgcctg ctcgtggagg tcgggcacgg
    12001 cgggatcacc acccgccggc tcgcggccga ggccggcgcc aaccagggcc tggtgcacta
    12061 ctacttcggc tcgatcgacg agctcttcgt gcaggtgctc gagcgcttca ccgagcgcct
    12121 gatcgctcgg cagcgcgaga tgtacggcac ggacgccccg ttcatcgaga agtggcggac
    12181 cgcgtggcgc ttccaggagg aagacctccg cgggggctac tcgaagatct ggttggagct
    12241 gcaggcgcgc tcgtggagcc ggccggagct tcggccgcgg gtcgcacggg tcgacgcgga
    12301 gtggcgcgcc gtcctgcgcg aggcataccg gcgtgccgcc gccgagtacg gcctcgacga
    12361 ggaggagttc ccggtcgagg ccctcgtggc gctcacgatg acgttcggcc agggcttcgc
    12421 gctggagcgc ctcgagggca tcgacgaggg ccacgccgag ctgctcggct ggatcgagcg
    12481 ctggatcgaa tcactcgaag atcgaagggg gtcccgatga ccgtcacagc acccgacgag
    12541 gtacgttgcc gcgacgagca gagccgagcg cgctatcccg acgagacggg gttcgtcgag
    12601 cgagacggcg tgcgggtctt ctgggagtcc tacgggcagg gcgagcagac cgtgctcttc
    12661 cttcccacct ggaccctcaa ccactcgcgc atctggaagg cgcagatccc gtacttcgcg
    12721 cgtcatttcc gggtcctgtg cttcgacccg cgcggcaacg gccgctcgga tcgtccgacg
    12781 gactccgccg catacgccga gcacgagttc gctcaggacg ccatcgacgt gatggacgcc
    12841 tgcggcgtgg agcgcgccgt cgccgtgggg ctgtcgcgtg gcgcgcagcg tggcctgctg
    12901 ctcgccgcgg agcacccgga gcgcgtgacc ggcctggcgc tcatcgcccc gctcttcccg
    12961 gtcagccgcg tgcggtcggt gcagcagcgg atggcgttcc acccgcgagc ggcggcgttc
    13021 tggaagaggc gcccgccgac cacgcgcggc cagggcaagt tcagccccta ctactggacc
    13081 cacgggggct acgccgactt cgtgcagtgg tgggcggaac ggatgctgac ggagccgcac
    13141 tcgaccaagc agatcgagga cgccgtggcg tggtcccacg agaccgacgg cgattgcctc
    13201 gcggcgagct tgaccgcgcc gctcgcggcg cccgccaccc ggcgcgacca gatcgcgctc
    13261 gccgaacgag tgcgctgtcc cgtactcgtg atccacggaa cgaaggaccg ggtcatgccg
    13321 taccccgacg ggaaggcgct cgcgggcgtc accggcgggc gcctcgagac ggtcgaggcg
    13381 gccgggcatc tgccgcatgc gcgcaagccc gtacaggtga acttggcgct gcgggagttc
    13441 gtggagggcg cgccgctgcg cgatccgacg gttcaccgcg ccgacgggcg caagcgagcg
    13501 ctctacatct cctcgccgat cggcctcggc cacgcgcagc gcgacatcgc gatcgcccgc
    13561 gagctgcgga agctggtcga cgggctcgag atcgactggc tcgcgcagga cccggtcacc
    13621 cgcgtgctcg agcgcgaggg cgagaccatc cacgccgcga gcgaccacct cgcgaacgag
    13681 tcgcagcaca tcgagtcgga gtccgccgag cacgacctgc actgcttcca cgcctggcgg
    13741 cgcatggacg agatcctgat caacaacttc atggtcttcc acgacgtagt gcgcgaggag
    13801 aagtacgacc tgtgggtggg cgacgaggcc tgggagctcg actactacct gcacgagaac
    13861 ccgcgcgaga agcgggcgcg ctacgcctgg ctgaccgact tcgtcggctg gctgccgatg
    13921 cccgacggcg gtgagcgcga ggcgttcctg acggccgact acaacgccga gatgatcgag
    13981 cacatcgagg gccatcccga cctgcgcgac cgggcgatct tcgtgggcaa tcccgacgac
    14041 atcgtcgacg agcggctcgg tccccagcta ccgatgatcc gcgactggac cgagcggaac
    14101 ttcgagttca ccggctacgt gaccggcttc gacccgagca ccctcggcga ccgtgacgag
    14161 ctgcgtcacg agctcggtta ccggccggac gagaaggtct gcatcgtcag cgtcggcggg
    14221 tccggggtgg gcggtcacct gctgcgccgc gtgatcgcct cgttcgacgc cgcgaagcgc
    14281 caggtgcccg agctgcgcat gatcgtggtg gccgggccgc ggatcgatcc cgactcgctg
    14341 ccgcagcacg acggcctcga ggtggtggcg tacgtccaca acctctaccg ccacctcgcg
    14401 gcatgcgacc tcgccgtagt gcagggcggg ctgaccaccg gcatggagct gaccgcgagc
    14461 gggaggccgt tcctctactt cccgctgcgc caccacttcg agcagaacat gcacgtggcc
    14521 catcggctcg accggtacgg cgccgggcgg cggatggact tcgacacggc cgaccccgac
    14581 gtgatcgccg gcgcgatcgc cgcggagatc ggccggcgcg tggagtaccg gccagtcgag
    14641 accgacggcg cgcgccgcgc cgcggagaag ctggcggagc tcttgtaggg agggaccgat
    14701 agtgcggtcg acagggaggg gtacgcatga cgcaggtgac ggagcacagg ggcttcaccg
    14761 agcccgacga ggtgcgcgag ttcccgaacg gcagggcgga gatcctgtcg gtgggcgatt
    14821 cggaggtggg ggaggctgac tctcgagccg ggctggcgct ggtcgaacga cgtgaagccg
    14881 atcgccggca ccgacagctg cgaggcgccg cacttccagt accacgtgag cggtcagctc
    14941 gcgatacgca tggacgacgg gaccgagttc gtcgcgaagg cgggcgacat cacctcgcta
    15001 ccgagcgggc acgacgcgtg ggtggtgggg gaggagcctg tggtggtggt ggattggtac
    15061 ggggccagta gctacgcgaa ggggggttga gcgcgcgagg gcgcccggct acccgatcgg
    15121 cgccgacttc accgtgccgt cgttgctcca gtagaccgtc ttcccgccgc tgctgacggc
    15181 gaaggagtcc gggtcgatct tgccccggtc gagggtctgg ggggtctcgc cccgcgcgat
    15241 ggtgcgcacg gcgacgtcgt tcgggtccgg ctcatcgtcg ccgttcttga agccctgcac
    15301 ccagacgagc ccgcccttcg cggtcacctg gaagctctcg acgtatccac ccgtgtcgac
    15361 ggttgccggc ccgctctgct tggagaaggg cacccgcttg ccggtcttca ggtcgaagac
    15421 gcccatctcg tcgacctcgt tgaaggcctc gtcgagccgg taggagaacc cgaggtagcg
    15481 cgagccgagc tggaatcgcg cgaactggca gcagacgccg cggagcttga cgaggcgcct
    15541 gtgcttgtac gtgcaggcca cggtctgctg gaggcggcgg ctctcgaaga tcaccccggt
    15601 gcggttcgcg acggtgaccg tgttgcccga cttcaacgac tcggcccggc acgagttgcc
    15661 ggcggccgcg gccggcgcgg gggcggtgat cgcgtaggcg accgcggcga gaaccgcgca
    15721 gaggtggatg gagcaacggc gcatcggcat gggagaacct ccggcggggt caggggggga
    15781 gcgggcgagt ccgcgggagc acgaccctaa cgggtcggac ccccggcgag cggggcgccg
    15841 aaagggccgc ctactgtccg tactcggcgt cggtcacgtg ctctccccag gtgaccgggc
    15901 tgcccgactc gtcggcctgc tgcatcgcga ggtgggtcat gaggcggtcg ggtgccgcac
    15961 cgtgccagtg gtcctcgtcc ggctcgaaga agacgcggtc gccggggcgg atcgtctcga
    16021 ccggcccgcc gcggcgctgg cagcgcccga cgccctcggt cacgtagatc gtctgcccga
    16081 gcgggtgcgt gtgccacgcg gtccgggcgc cgggggtgaa gtggaccatc gccgcgttca
    16141 ggcgcgacgg cgccggcgcc gcggcgacct ggtcgatgtg gaccgtcccc gtgaaccagt
    16201 cgctcggccc ggtcgcggtc tcgagggagt tgcgcgtgat ctccattgcg ctcacaccgt
    16261 cccgacgcgc acgagcgact tgatcgcgcg ccgctcgtcc atggccgcgt aggcgtcggc
    16321 gatcccgtcg aggccggtct cgtagtcgaa gacgcggccc ggatcgatcc tgccctcgag
    16381 gacgtcgccg agcagctccg ggatgtaggc gcgcgcgggc gcgggcccgc cgagcacgcc
    16441 cttgttgcgg tagaacatct ggtccacgga gaactccacg ccgtgcggca cgccgacgta
    16501 gccgacgacc gatccgggtc gcgcgatcga gagggccgtc ttcatcgcct gcccggtgcc
    16561 gacgcattcg agcgccgcgt ccacgccgat gccctgcgtc agctccatca cggcctcggt
    16621 tgcctctctg ccgcgctgct cgacgacgtc cgccgccccg aactcgcgcg cgagctcctg
    16681 ccgcttctcg tgacggctca gggcgatcac gcgctcggcg ccgaggcggt tcgccgcgag
    16741 aaccgcggac agcccgaccg caccgtcgcc gacaaccgcg accgtgtcgc cctccttcac
    16801 gcgcgcgcag acggccgcgt ggtggcccgt cgccatcacg tcggagagcg tcagcagcga
    16861 cgcgagcatc tcctcggagt ggcccgagcc gggcacggtc acgagcgtgc cgtccgcgag
    16921 cggcacccgc acggcctcgc cctggcctcc gtcgccgttc atcgggaaga acccgccgtt
    16981 cgagcacgcc gtcgtgatgc cgtgctggca gtgggggcag gtgccgtcgc tgtaggcgaa
    17041 cggcgcgatc acgaagtcgc ccttgctcac gttctgcacc gacgaaccga cgtcctcgac
    17101 gacgcccacg aactcgtgcc cgatcggccc gggctcgaag ggggtgtccc cgcggtagta
    17161 ccagaggtcg gagccgcaca cacacgcgag cacgacgcgc acgaccgcgt cggtcggctc
    17221 gacaagagag gcgtcaggtc gatcgccgac ggtgatggag cgggtttcgt tgaagatggc
    17281 tgcgcgcatt cgtcagagcc ttcggtcgag gtcacggggc gagcggaggg tgtcgagtcc
    17341 gctcgctgat cacggtacct gccgtcctct tcgcagggac gccgaacgca accggcaacg
    17401 gatcctcacc gcggccgccg aggtcttcgc cgcgcgcggc ctcgacgtca ccctcgacga
    17461 catagccgct catgccggcg tcggcgtggg gaccgtctac cggcgcttcg ccaacaagga
    17521 cgagctgatc gacgcggtgt tcggagagca gctcgacgag gtggccgcca tcgtctccga
    17581 cgctctcgag gctcccgatg cgtggacggg cctgaccacg tacctcgaac ggtcgctgcg
    17641 catgcagctc gaggatcgcg ggttcacgga gatcttctac gggaggtcgg ggcggcggct
    17701 cgacgtggcg cgcgagcggc tcgctccgct caccgagatg gtggtcgacc gggcccgccg
    17761 cgagggggcg gtccgcgagg acctcgagcg cacggacctc gtgttcgtcc agctcgcgct
    17821 ggccgcgatg atggacgcca cgcggggcgc cgagccagag ctctaccgcc gctacctggc
    17881 gctgttcctc gacggtctcc gcccggcccg ggagggtccg tccgcgctgc cggtcccggc
    17941 gctgagcggc gagcagacgc acgccgtcat gacgcgcgga gggtgagcgc cgcctggctg
    18001 ggcgcaggta gtctgtagca catatcgtgc tacgctcacg tgcgctgtga gccggtcgat
    18061 tcacgttcgt ctcgacgaca gttctcttgc tgcgctccgt gccgtgacgg gaaccggaat
    18121 gagcgactcc gaggctgtcc gaactgcgct gcgggaagcg gctgcaaggc gtcgagcccg
    18181 atcggccgtc gccgaagagg tccagcgtct cgcggatgac gtggccgatc gcgaggagat
    18241 gcgcctcata cgcgagcaga tgtcggaact cgcgcccgac ctgcccgact agtggtccgc
    18301 ggcgaggtct ttcggctgcg agcacctcgt ggagtg
//
LOCUS       DASVPL010000298        43747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_75232, whole genome shotgun sequence.
ACCESSION   DASVPL010000298 DASVPL010000000
VERSION     DASVPL010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 43747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43747
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75232"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VF517_16780"
     CDS             complement(<1..1264)
                     /locus_tag="VF517_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054644.1"
                     /translation="MLRLLSGALTLLALAAAAPVAPAQEPRLARIVYSADGRIQTIAA
                     DGSDRRLVAAEGAGEAAWSPDGDTLAWTRFEVDEDSEGSRVWLSAPDGGGARPLTAAP
                     TTGGLELSPTWSPDGRRVAFVRVRYGEERIVTTLVSAAAGGGDEHRVARIASSRYVGM
                     LAADWSPDGARILVTVGDLSGESSDPPELHVVDAAKGTRRRLLRRAGEGRWSPAGDRI
                     VYVGVRPRCEEDACEELYVANADGGGRRRLTTNEARESTPTWAPSGDRIAFVSDRNYP
                     RGGSLEVYSMGADGSCLTWLTNGTASPFAPDFEPGAGLSSDPGACGATPREPLIETDT
                     SELASYRDVPIWWLGPRFGDLLLSDAEEESGNADLSYDDCARYEPSDCPPPIELSNLP
                     ACSTSFGFGGGASLVRHGGALLDTQRFGD"
     gene            complement(1258..2961)
                     /locus_tag="VF517_16785"
     CDS             complement(1258..2961)
                     /locus_tag="VF517_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054645.1"
                     /translation="MGRGSRAGGVVVVLIALATVATASAQLPATPTEPSPMTTPAKAR
                     IVFNAGYGLRTLAADGSDMRQLTTRSDEGGDSNAAWSPNGESIAFVRSQLAADHEDAE
                     DLRVHVIRADGTGTRPITAKASTVRWEHDPAWSPDGRQLAFVQVREGRTRVVASIIAV
                     NLDDGQRRTVYSETYESSDEEGAPYLQTPAWSPNGETVLFTRSTFGGRDEEDYRPSVY
                     AVPAAGGEARLLIRDAAHGAWSPDGGRIAYSGMHDRLGRSCREECIVAGEIYVASADG
                     SNRVRLTESRADDDDPSWSGDGQRIVFASDRNAVQAETEEAGREIYSIRPDGSCLTWL
                     TNGTADSYAPSVQPGTGLSGDPGGCGPSAREPLVETDMRAIDSPRPFRVWWLGAVAPN
                     GLLVTDVQASKSSVEVAYSDCGRFDPDECGEFLLLMNDDLCFAGRILGGARPGGIETF
                     HGALLYTERDTELSWSHVYAHRTRVEGITESGRAVPPAVLDHLRPVGGERAAGAPFPA
                     ARLPNRYWRLLTSVANAYRRTRSLSAVSRRLRLKRSEVKRRLAVGRRLAQLGVRRRLG
                     C"
     gene            complement(3001..3969)
                     /locus_tag="VF517_16790"
     CDS             complement(3001..3969)
                     /locus_tag="VF517_16790"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX8054646.1"
                     /translation="MNSILVAPDSFKGTLAASEVAAAVAAGLRDGGVEAVELPVGDGG
                     EGTMDALLATLGGERRRARVSDPLGRPLEAEYALIDGGERAIVEMARASGLGLVAEGE
                     RDAFAASTRGTGELIAAAAADGAREVIVTAGGSATTDGGAGALEALDDAGAAPKLTVV
                     CDVRTPWELAPRVFAPQKGADPETVKRLERRLARLAKAAPRDPRGVPMTGAAGGLAGG
                     LWAHLGASLVPGATFVLDTIGFDDAMRAARFVVTGEGSIDHQTLAGKVVFEVGTRCRQ
                     AGVACHAVAGRNELAPFEARLIDLATITEAGTKRRLRSAGRRLADL"
     gene            complement(3966..4463)
                     /locus_tag="VF517_16795"
     CDS             complement(3966..4463)
                     /locus_tag="VF517_16795"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX8054647.1"
                     /translation="MPELEVIQGDVTKLAVDAIANAANTRLRHGGGVAAAIARAAGPE
                     LERESRERAPIGLGEAVETSGGELPARWVIHAATMELGGPTSAEIIERATDSTLAKAD
                     ELGCRSLALVAFGTGVGGFPVGDAARIMVGAVRRWEARSLQRVVFAVHGDEAERAFRA
                     AVDAG"
     gene            complement(4466..5428)
                     /locus_tag="VF517_16800"
     CDS             complement(4466..5428)
                     /locus_tag="VF517_16800"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX8054648.1"
                     /translation="MSLPLKPPIKPQLARPAKDLPAGDGWFFEPKWDGFRTVVFRDGD
                     EVHLQSRNGKPMNRYFPEIVPQVLSLSPERIVLDGELIVVVDGIQEFDLLGQRIHPAK
                     SRVEMLAEQTPAGFVAFDLLAIGDETLLELPYTTRRERLAEAVAGSEVQLTPATDDAE
                     AARIWLTGQSEGVIAKVADAPYLPGERTGMRKIKRVRTADVVVQAFRFGKEPNTVGSL
                     ILSLYDEDGQLHVVGHTSGFKAKEKRELIDLLEPYRTYERGAGEASRWKSDEELVWEG
                     LRPELVCEIGFDHITGNRIRHGAKFHRWRDDKSPEECTMEQLRA"
     gene            5454..6167
                     /locus_tag="VF517_16805"
     CDS             5454..6167
                     /locus_tag="VF517_16805"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX8054649.1"
                     /translation="MSSQQATVAEEEYLQTIYWLFEAGLPMTAANVVRAMQVSAPTVH
                     EMVGRLERDGYIVRGDDRSLAFTDEGREHAAGAVRRHRLIERFLTDVLGIPWDEVHEE
                     AERLEHAMSPVLEERMLAAIGDARTCPHGHPIEVGARLPGVPLADVEEGARVRVLRFE
                     NEAEDLLHYLKGTGLFPGLEGTVAVSDDEEVVVEAGGERRVLTRSVAETVTVTADPSP
                     PPRVALPEQLVLARERYGR"
     gene            6259..8163
                     /locus_tag="VF517_16810"
     CDS             6259..8163
                     /locus_tag="VF517_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8054650.1"
                     /translation="MTRLARAVCCSLLLLFVLAPASHAAIAFEPCGERTELQCGTLTV
                     PIDRSGSVPGTIKLQVTRVPAVKPSGKAVFAFAGGPGQGATPFADSFAAQLGRARRTH
                     DIVVFDQRGTGASAPIDCTDVDRVRDIRDAAAQIRACGEKLGPTAPFYTTADTVQDIE
                     SIRAELGYEKIGLFGVSYGTKVELAYASTFPQRVSRMVLDSVVQADGPDPLLRDSFRA
                     VPRVLRELCRSAYCRGVTTDPVADMAAAVARMSGGPLTGSTYSVKGKREAASISSSTL
                     FTLLLIGDFETALRGAIPSAVRSAALGDPAPLLRMAKLIEPPPNPQAPELSEFSTGLW
                     LATVCAELPFPWDPDAPESERRAQAQQRSAALDPAQFDPFDPGTALSWDIVEMCQHWP
                     VARRPATGDLSKVEAPVLLVNGRIDLRTPLENAERMAALLPNATVLPIPEVGHSVSDA
                     DPTGCAGLATDDFLAGRTLVPCQNAPPRMRIRPFAPTDLNALSPSKGTSGKRGRTAKA
                     VDLTIHDVIAELDLFYADRGGLRGGSFTLGDEDIVFRGLVYVPGVKVSGRLNAFSYGG
                     TLRISGSKAARGTLRVGRKGAVTGVLDGRRISTRIKLPKPPEGLGGGGGEEGPGEPRV
                     PTWLGPLLGR"
     gene            complement(8182..9282)
                     /locus_tag="VF517_16815"
     CDS             complement(8182..9282)
                     /locus_tag="VF517_16815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX8054651.1"
                     /translation="MESTAPITFARGAPSLDIVAVEDLKQAAQRAFENDPSGAFMYGT
                     SGGYPALRDWLAQKHQIAREQVIATNGSMQADAFLFQREVKSGDTVVIEAPTYDRTLL
                     VLRNMGADLLPVPVESDGLDVEYLARELEAGKRPKLAHVIPNFHNPAGVTLSLEKRRR
                     LLELAGHYDFMIFEDDPYGELRFEGESLPTMFDLDDAERVVYASSFSKTVCPGIRVGY
                     LAGPPAVIGDIERRATSTYISPSMVSQSIVAEFCTSGAIDRSVETVKNALRERRDALA
                     ETLQSELPDARFTPPEGGYFLWVELPEGADVDALAAAAKDRGVIFVKGTDFLIEGGLN
                     TLRLAYSGVGPDQIRAGIGRLADAYKNLSVTA"
     gene            9397..9882
                     /locus_tag="VF517_16820"
     CDS             9397..9882
                     /locus_tag="VF517_16820"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8054652.1"
                     /translation="MEQKIVPNLWFDGQAEEAANFYISVFKDGRILSKAHYTEDSPGE
                     PGTVMVVEWELNGQRFVGINGGSQFKFDEAVSFQISCEDQEEIDYYWDKLSEGGSEGP
                     CGWLKDRYGLSWQVTPKGMDELFSGRDEERANRAMKAMLKMSKLDIAELRAAADGVPA
                     A"
     gene            complement(9879..10487)
                     /locus_tag="VF517_16825"
     CDS             complement(9879..10487)
                     /locus_tag="VF517_16825"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8054653.1"
                     /translation="MAVGSDLVRHRIPLPGGELTILQPYEAAELPDDGPVEWAPLLPY
                     WSVLWRSGLALARELEGAELEGLRVVELGCGLGVPSLVAARAGATVLATDADAEALEL
                     VRQNAAENDLDLTTLRVEWAAADSLVALGPFDLVLAADVLYERASAAPLQSLLARLGG
                     EAWLADPGRPAAEPFVKQARRHWSVATSVRDEVEIHRLRSRR"
     gene            10571..10930
                     /locus_tag="VF517_16830"
     CDS             10571..10930
                     /locus_tag="VF517_16830"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8054654.1"
                     /translation="MNYMLLIHQGDTPTPQTPDEWAKLSEDERNAVYADYKAINETPG
                     VTPGLGLQPADTATTVRVADGETLTTDGPFVTVKEALGGWLMYEADDLDAAIELAARI
                     PAARMGGAVEVRPTQEW"
     gene            10924..12102
                     /locus_tag="VF517_16835"
     CDS             10924..12102
                     /locus_tag="VF517_16835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8054655.1"
                     /translation="MVAIALEDVFRDEWGRVLASLIGFLGDFELAEEAAQEAFAIAAE
                     RWPTAGTPANPRAWLVATARNRAIDRIRRERVGAEKARLLETVPQAMDDPFAHDDTTI
                     RDERLELIFTCCHPALAVEAQVALTLRSLGGLRTEEIARAFLVPEATMTKRLVRARRK
                     IRDAGIPFRVPADHLLPERLAAVLAVVYLIFNEGYGGRGELATEAIRLGGALAELMPD
                     EPEAHGLLALMLLNDARRDARFAGGAVVLLADQDRSLWDARQIELGRTALDRAVALSG
                     SGPYVVQAAIAALHVDEHPDWAKIAALYGTLAQMTGSPVVELNRAAAIAEAGDAEAAL
                     ALVEGLDLDGYHYLHTTRGELLCRLGRVEEAGVAYRRALDLVHSDAERRFIAGRLAEL
                     "
     gene            12129..12509
                     /gene="tsaA"
                     /locus_tag="VF517_16840"
     CDS             12129..12509
                     /gene="tsaA"
                     /locus_tag="VF517_16840"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX8054656.1"
                     /translation="MESPLTDLASAPRQGDEGAPDAWLVFEPAVLDALDGVGVGDHVI
                     VLTWLDRADRSVLRVHPRGDPSRAEQGVFGTRSPDRPNPIGLHRVEVLSIEGARVRVS
                     GLEALDGTPIVDLKPVLGSDPGER"
     gene            complement(12530..12841)
                     /locus_tag="VF517_16845"
     CDS             complement(12530..12841)
                     /locus_tag="VF517_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054657.1"
                     /translation="MTERPVEGINITEVGDRVVIEVDNGRWEGTAAQAEQLLTRLHYV
                     LARARPDTNIASIFLNGELWGGKFTETDSQAAIRKMRHTIDQLADYVASLESGAGGTQ
                     T"
     gene            complement(12861..13136)
                     /locus_tag="VF517_16850"
     CDS             complement(12861..13136)
                     /locus_tag="VF517_16850"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX8054658.1"
                     /translation="MSALSVEPGTEFPLVREPDRVLRNEYLVVIEGGDTGWSAYVPDL
                     PGVIAAAGSEQEVRSLIDEAVEFHLEGMREAGLPIPAPMSQAHSVFG"
     gene            13416..14267
                     /locus_tag="VF517_16855"
     CDS             13416..14267
                     /locus_tag="VF517_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054659.1"
                     /translation="MPSVRASPLPSRPLAIVPERLESCVRRLDPADRALLDLSLNRGI
                     PDAAMAPILRTDPLRLAWKRARAIERVASRLGLTHPADLGLVRTALADLPSRAWLPLE
                     LQPAKQPRVLEPPEPVGEEPRVMKPSVQPAATVAPPEPAGAEPRVTRPAADERPGGGT
                     APATPPEPPGAEPVVFRAREPEAVRASLRAAAQPGTALAPARPAQAAAGLVAKPVSTY
                     GRGLATPARAEDSGVQFGRAASFTLGGLSERAKRAYPRGGMTTRARRAAGALAVGAAA
                     LLALRRS"
     gene            complement(14254..15135)
                     /gene="sucD"
                     /locus_tag="VF517_16860"
     CDS             complement(14254..15135)
                     /gene="sucD"
                     /locus_tag="VF517_16860"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX8054660.1"
                     /translation="MSILVDKETKLVVAGLTGREGSFHGLNNRSYGTDLVAGVTPGKG
                     GQDVEGVPIFDTMHAAVDEAGANTAMIFVPPRFAADSILEAADAGIGLVICITEGIPV
                     HDMLRVYTHLRRGSTRLVGPNCPGILSPGKANVGIIPAHFFSEGNVGLVSRSGTLTYQ
                     IGNELAQRGFGNSSIVGIGGDPIAGSSFIEIITLFEADPETELIVMCGEIGGEEEERT
                     AEYVRENVSKPVLAYIAGFTAPPGKTMGHAGAIISGSKGTAAAKAEALEAAGIRVGRT
                     PTQVAELAAEVAAATTA"
     gene            complement(15132..16286)
                     /gene="sucC"
                     /locus_tag="VF517_16865"
     CDS             complement(15132..16286)
                     /gene="sucC"
                     /locus_tag="VF517_16865"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX8054661.1"
                     /translation="MDLLEYQGKQLFRKHGVPTPDGKPASSVEEAVEAAEALGYPVVI
                     KAQVLIGGRGKAGGIKLAKDRGEAEEHSKAILGMDIRGFTVHELYVEKASDIDEEYYA
                     AILLDRTAKKPLAMLSRMGGMDVEEVAEKDPKALVRVHIDPLVGFSDFHARRLAFEAG
                     IADDVVRPVGALLAKLYDAFVAEDAVLMEVNPLLVTGDRQVVALDAKVTVDGNSLFRH
                     EDTAAFRNVSDEDPQERMAKERGLTYVKLDGDIGILGNGAGLVMSTLDVVAQAGGKPA
                     NFLDAGGGSKADAIVDAVEVILSDEKVRAVLFNIFGGITRCDEVARGLVTAFGQIDVK
                     VPFVVRLDGTNDVEGREILAEAALPNVTVEKTMDGAAAKVVELAKAGAPA"
     gene            16390..17070
                     /locus_tag="VF517_16870"
     CDS             16390..17070
                     /locus_tag="VF517_16870"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX8054662.1"
                     /translation="MPIPKDVRFVTFDCYGTLIDWESGAYDAFQKEADRDGFTIDKNS
                     LAPKFIEAQHEIERGSYELYAEVLRRTAVRCAKELEWDLEPSRSGFLPSSVPYWPPFR
                     ETNAQLERFAKKFEIGILSNVDDKLLGATRRHFRVDFDLVVTAQQVRSYKPDPAHFKE
                     CARRVGSKSGWVHIASGYDTDIEPILKMRVPVIWVNRRGVKLEPGQKKPTEEVKTLRD
                     AAKLLKVA"
     gene            17105..17857
                     /locus_tag="VF517_16875"
     CDS             17105..17857
                     /locus_tag="VF517_16875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8054663.1"
                     /translation="MVTSRLWQTNATAIRRGEECLLIDSPYFPDELELLPALLAQAGF
                     EPDGLLATHADYDHLLGRLAFPGLALGVGWPTAERLRSEPGAAQRELRNADAEHYVAR
                     PAPLALGQYQELPVPGSIEIGDAELELHATDGHAPDGTAFVARFAGLLVCGDYLSDVE
                     IPLIARAGSLDDYRATLARLTPLVEAAETIVPGHGSPHDRETALRILDEDADYLDALA
                     AGEERPPLPASRDTSRQRAIHADNLAKHGPAA"
     gene            complement(17854..19998)
                     /locus_tag="VF517_16880"
     CDS             complement(17854..19998)
                     /locus_tag="VF517_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054664.1"
                     /translation="MAICAVASAAPVLTSQSGWSWGNPRPQGNELKAVDFAGGRGYAA
                     GDFGTVLRTDDGGTTWSGVRTGLVDDLSKLRVIDASTFLVGGGCTLLRSDDSGQTLRR
                     LRFNPSANCGSPLAAMHFATKDIGFLIRSDGSVLRTEDGGARFASRTALPATGGGPPN
                     DAWFTSPDNGLVVTGSDTLGRIYRTTDGGSSWKDVATSGALRGLYFVSATTGYAVGQN
                     AVLKTVDGGANWTTTDASVSSPLRSVRCADELHCVVIGGNGLVLHTDDGFETLEPSTY
                     EPPPGGPSGTLPIPGLAASYNSATRVAVVGRRGEAATSDNAGDTFTPIGSTLQGAYNR
                     LRLTSQSTVFAPGSGGSVARSTDAGATWSRVGAPTTNDVVDVAFPTATLGYAVDTEGA
                     VFRTDNGGTSWAILGDAGVRPRSITASGDGNVVMLIGPRGMVRSANGGANFDPVDAAP
                     VGPKVGLDDVDRTPDGGVYVSGPKALAFSGDEGAKWAALRRPSRSTILEVDFVDKKLG
                     YVLTADGRVWQTANRGGKWKELLTVGSADAYEMAFGDKNTGYLAVRDFGNFNGGWVLR
                     TSDGGASWRPQLISNDNITSGLGSLVAAPGDIAHALVGTSDLFSTASGGDAASGSTIT
                     LTTKTKRLRRKGKVRLDGKLAPAAPGARVQVSVRELGSGRWTRQFVTVRSDGTFITNW
                     TVKRSSSFVAQWAGDQALNGDGSPALRVTVGG"
     gene            complement(20147..20596)
                     /locus_tag="VF517_16885"
     CDS             complement(20147..20596)
                     /locus_tag="VF517_16885"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEX8054665.1"
                     /translation="MTDYEAMRAGLQEAIPFNKHLGLEVVEIAEGRGVVRLPDDGELH
                     NHVGSQHAGGLFSAGEAASGAAFVSVFAEHMGSLTALAKSAKIDYRKLAKGPITATGT
                     LGGDAGGLLEQIESDGKVEFPVEIEMKDEDGQVVASMSVRWHVRRNS"
     gene            complement(20609..21784)
                     /locus_tag="VF517_16890"
     CDS             complement(20609..21784)
                     /locus_tag="VF517_16890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8054666.1"
                     /translation="MPRRRDPRALIPLVLATLAAQASIMVLAPIMVEIGHGLDASVSA
                     VGQARTVMAAVAAVASLRVGPLIDRLGVRPVITWGAVLAVTGHAITSTAAGLPVFYLG
                     HAFIGLGVACLLSGGFAGAGAWFDEEDAPWALGYVVGSQSIAWIVGNPVIGVLTDAVS
                     WRLAYAVPGAAALAALATALLTRVRRGEEQTESTPSGMTDVARDPSARAWTIAEFVAY
                     SAWTAELTYIGAFYVETYDVQEALVGVLLAIGSLAFLISTLGTDRLVRRLGGRRRRLV
                     VTAALAMGSLVALIMNVTPAVAFTVAMFFLMALCAGVRSTGSSALALGLMPAQPGAMM
                     AARTTSAQLGYTIGAMFGGLVLAVGDFGTLGFLLFGGMAFSAVLIARVAEPQETVSR"
     gene            complement(21813..22856)
                     /locus_tag="VF517_16895"
     CDS             complement(21813..22856)
                     /locus_tag="VF517_16895"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX8054667.1"
                     /translation="MPDYNPEQIEVPGWGSEFPPEQPRRDVPESLDAMPTAAAVAAAS
                     VTGEDVRVNPRLEDRRRLDRRRLFAHVIDEIVLALGALVGFFLFAGAAVGMLMTYLAL
                     KLSYYMVLETAFGQTLGKKAMRLRVVGLDGNGVPANRIAARTIFRFVDDALAVAVFPI
                     VGGISLLLTGPRRARIGDIAAGTCVRDNDRPFKAAPDSPLIVVYPVLWIGAALAAGAA
                     LPDKPLLEYFRSEHPYMAKIDKICEKRVRQTEAMKSLEGNNILTFRIFFRQEQRKIEQ
                     LPKPPADVRADVKEVIRLHSQLNRALDRALRDARSAPNPDAALATHVPQIQLLAENAA
                     ERFAELGLPYCTQ"
     gene            complement(22892..25354)
                     /locus_tag="VF517_16900"
     CDS             complement(22892..25354)
                     /locus_tag="VF517_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054668.1"
                     /translation="MPAPSTQPAPSGVDANQPPPAKRDTEPVILRGSDLPLWSARSNQ
                     TFRLPLVDFQCQANQLPKEFPKPPNTPPCPHNYNPQPDADTRDFLGAGPAVDSLLGYR
                     YDAAKKTWKQIPFQVDEVFTRYLNNSASGFAIYSGEDQHTTYAYEREGFRWFEEDPKN
                     PCLARPADEPAKDPVPGLDDNDELAFMYTDAGDRIGANTPLPAGIDGAPYEVVVNDPT
                     GREGTKYVYVMQASPSGPKPAYTAENGYVEYKRDKVSDTFERSVSSYDDYGNAREGVY
                     CDKDGNVVIDPKKGKPAIDKRRPRDYATIKTDRYRFRYDGRWLMTEINISPDKGKTYA
                     PDVVDRWKARAFAQDPGSETPCCGFEEEDTNWGGSSTLLGEKVGPVRAIRETWGADSG
                     TNVVRRESFYRHEMRQKTWLRVHVIPPLDGIYAQWDFNANRMKRFYNSSRLDGVDVDG
                     RNDEVFGNFDDPCNSNWDQNETSQLDEGYRTIYQELDVCNGAIDCPPEAGQFPDGPQG
                     VNPVESLCGRPYHQSIDAFDPTFSKANVALEWNEIAGPYGTIVDRITTDVRDLSPGGT
                     AQSVVATPYYRDDSCFDDGTGSDPGPKVKLRSGDEPRTTSTGEQRRCWKPGDGEPNSA
                     EKFFQGSIGTHGLHLLFLVDSDNARQTVPLNEIVSEWRMVMLPGDQTANGRGPEAGQE
                     YGETFDNPAQPGSQPVPPAEGGGGSGQPGAGPSFVDRTAPTVRLKARRHPSHHRIHLT
                     YSVFDTGGSGLKTLKIQVKSGKKWKTIRTGTKRRKWNYKARKSGRYTFRLTAQDNAGN
                     TSKPVYARVSIKNSRFRGQHSH"
     gene            complement(25489..26079)
                     /locus_tag="VF517_16905"
     CDS             complement(25489..26079)
                     /locus_tag="VF517_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054669.1"
                     /translation="MSAGSSAAGAAVGLALLAGLASGCGSNDAPAQGAADVSAARQFK
                     PFALYYAGPSFEELPLTNGGGDDVEDSPRGPISFVYGTCEPEPGAETSCAPPASIQNF
                     PICKENLSRYRGRARPRMLSLRGAPVAVFSDGSGAFEKVEVFAGRTTVALWVDDVETA
                     KRMIGELEPINGRRRGRRLPPPPRGAVEGRRPACGD"
     gene            complement(26063..26776)
                     /locus_tag="VF517_16910"
     CDS             complement(26063..26776)
                     /locus_tag="VF517_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054670.1"
                     /translation="MPVISRAPKRSAVAWVTLVVTALALSAAVSPSDSHAFWRGGSWS
                     YPDGSDCKSSRQRIDPIGAIFHGTAAHWGFIGSYDAGTKVHHGHIEKHTGWDYNTKGV
                     QKMAHSGGCYKMIGTMGKHKSDTLDYHARFWEHLDPPKDKYGKHSVVPATPHMDEHDH
                     GKCQGDHVPRHMDLPVGGTGSGYDYARQRLYLALKTGEHKHFARTEYWGHTAPVNQCG
                     GKYQVHGSGHVAIIRVGRK"
     gene            complement(26767..29304)
                     /locus_tag="VF517_16915"
     CDS             complement(26767..29304)
                     /locus_tag="VF517_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054671.1"
                     /translation="MEQPDWWVQPVYDQGFGTFAWDEACEGVLIDPGQFNHCPAFVSA
                     ATGGGDLFVSTSYPDARVEVYDGATLAHKLTISDAALGEPRGITYYRGEVFVMDGSGA
                     DILVFDAASGSLRRRFTVPAGFGEYVWQLMPTGVDAAWGELWITYMGGLGGGAVVVLD
                     SQTGAEKGASWHVQRLDSTAGELWWDIATVPEMNGVLAQCRFASRVEAVQPVPLDEVQ
                     ADEATGGNACSSKGEQGGIDAVWGMRWFLSVQEPRDGGPLAVNEFAIEPGGATAARLS
                     PLRRSWRPRMNEPNGGEFVHHDVAYQPRDARVTWTGALTKSDWMHGTRCADYVVSDAD
                     IYVVGTHAERWYEPARGFQQIDLLVDGAVRGTQTTPTGQFCFDTNSVPSGTHNVQLRA
                     KVNNGAKTVHVTNPSANWDHSPPDGGLDSLPRFVSGDVTVSGPMTDPHSGPMDWQAQV
                     QRVGGSWQDLCAPVPTAGAADGKYSCPWSTTSFSDGDYNVRAQKRDLVSGAWGGANVG
                     WTPEAVVGVDNSPPSSIGLSGELRDAEDYRPNADGDRPFLDVAVSDAGSGGTGADLLV
                     DGVSVASASGACPGGGCTFTHRFEFVPENFSDGEHTISVTGRDALGHTRTSESWIIDV
                     ERVAGEAPDHGDPGTESDDPAGGAAASRSDSAAADEVAAALGLLPCTSEDEPANFPTY
                     SLGSSFDGMPITAINRICEVPSPHELPRPNFVEFIYGTCTPDPVLGACQPPVVVQSWP
                     ACERNLSSYAQSGIEDFQLDYVLTTLRGVPGALFDTALRAEVYTEDATLVVFGETADQ
                     TSRAVSALRQEPAGAAPGALAPINRLDIGGKLPPPVPGALKGRLPCQ"
     gene            complement(29474..31255)
                     /locus_tag="VF517_16920"
     CDS             complement(29474..31255)
                     /locus_tag="VF517_16920"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX8054672.1"
                     /translation="MFSKVLVANRGEIAIRVMRALEEMGVATVGVYSESDRDALHVRR
                     AGESYLLGPGPATESYLVIEKIIDAAQKSGAEAVHPGYGFLAENAPFAKACEEAGIVF
                     IGPPASAIEAMGSKTRAREIMKNAGVPIVPGTTDPVDTVDDAAKIAEEIGYPIAVKAA
                     GGGGGKGFRVALEASELEKAFDGAAREGEKFFGDATVYLERYLPDPRHVEVQILADSH
                     GNVIHLGERDCSVQRRHQKLIEESPAPAVDEALRERIGSIGTEAARAVGYRSAGTVEG
                     LLQDGEYFFLEMNTRVQVEHCVTEMVTGVDIVKEQVRIAAGEELSLGQEDVRLDGHAI
                     ECRVNAEDASKTFAPAPGRIAAYREPAGPGVRVDSGVEAGSEITPLYDPMIAKLIVWD
                     VDREAATRRMIRALREYELAGLKSLINFHVALLDTPQWAAGETCRDLVEDKKWLKENA
                     APDPPAAPPAAGEDEEEKVEREYAVEVSGRRFDVKVIGAGLGGGNGGAPAAAATGARK
                     APKRRERGGGAAAGAPGELVSPIQGTVLKVAVEKGAEVTEGALICVIEAMKMENEITA
                     PAAGQVEELNVSEGGAVATGDTIAVIK"
     gene            31468..32478
                     /locus_tag="VF517_16925"
     CDS             31468..32478
                     /locus_tag="VF517_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054673.1"
                     /translation="MLSGRPLQGTSHDLDLFVDREGVMDRIHDSAARRLNTLVVGERR
                     SGKTSLLRVLQGRLASRFHVVMLDAGLSHDAASFLSLFLFSLEREAALGRAGAVSNPR
                     LDLDYSFALPATETERVLTLLDAVRHAIERRERGVVAIVDDLRTAELAHRLFGRMRDE
                     LWMLGCTWIVAADTQDRSALLRPPADAFFETVIELGPLDHRDAAELVRRRLADEDADP
                     DLVERLARAGEGNPGRTIELAREVVVDGIPLGEATSRRTDVRFRAGKLGDSARRLVED
                     LLTYGPASASDESLLRRLNWTRARATQVFKQLEAEGIVGAVPARSGGGRKVYGLNLNG
                     EA"
     gene            32562..33536
                     /locus_tag="VF517_16930"
     CDS             32562..33536
                     /locus_tag="VF517_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054674.1"
                     /translation="MPFDTLNGDERTESALARAAVAMERIALIGDGGVGKSSVISHVF
                     RLGSGFAPVVVPVFDERAAVVSDPGAFAAHAVQVLARYADQVELTAPRERDRYLRAIA
                     ERTHLPSTERGLRAGIKLAPWIAQFDVGADLKRVTPPAEIQPSASQYVAALDDMIAVV
                     KRTELTPVLIIDDSDRWLRLDDGEPRNDVVEPFLAHVLRMLADRAIGFVVAIHRTYVD
                     LAGYASAVEQGTLETFIDVPALGSGVALEALLQHRVQLHAPEASLADVFTGAALSELW
                     TVYRGGADGQIRKTLQVAHGALADADASTLEGPIDRPLVQASAVAALR"
     gene            complement(33539..34480)
                     /locus_tag="VF517_16935"
     CDS             complement(33539..34480)
                     /locus_tag="VF517_16935"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8054675.1"
                     /translation="MDPLIILFGLGVGILVGMTGMGGGSLMTPILILVFGFKPVTAVG
                     TDLAYGAVTKTVGGWRHFRQKTVNSSLSTWMALGSVPAAISGVYALAAIERASGRNFD
                     GAMLAVLAGALLFTGVAVLARALFLPKLVERERESFVLERRHKISAVVLGVFVGFVLG
                     VTSAGSGSLIAVGLILLFHLAPRQVVGTDVFHAALLLWAAAIAHVVAGNVDYALAGTI
                     LLGSVPGVWIGTSWSVRVPVATLRTVLGVVLIGAGLALAAKAGAGIPTPVIAGIPAVL
                     AAIVCGPPLRAWLRERRQAAARIQVPAPPRHVTAPER"
     gene            complement(34492..35559)
                     /gene="sat"
                     /locus_tag="VF517_16940"
     CDS             complement(34492..35559)
                     /gene="sat"
                     /locus_tag="VF517_16940"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX8054676.1"
                     /translation="MSSLKLTARQTADFELLANGGFAPLTGFQGSAAWETVTETMRLP
                     DGAPWPIPVTLATDQGEVGETLTLVGSDGTELGTLAVEEVYERDLAREAQQVYRTTDE
                     SHPGVAALHREGARTIAGPIEAKALPGHIDAVAPYVLSPKETRRTFAERGWNTVVGFQ
                     TRNPIHRAHEYITKCALEIVDGLFIHPIVGETKDDDIPADTRMRCYEVLIEGYYPSDR
                     VLLGVNPAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVGDYYGTYDAQHIFDEF
                     EPGELGIEPLKFEHSFWCKLTSGMATAKTSPSGPEDRVFLSGTKVREMLGRGERPPEE
                     FTRPDVADVLIAAYAGEPTPA"
     gene            complement(35556..36143)
                     /gene="cysC"
                     /locus_tag="VF517_16945"
     CDS             complement(35556..36143)
                     /gene="cysC"
                     /locus_tag="VF517_16945"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX8054677.1"
                     /translation="MPSPSAGCTANPLRRSDQMSEGLTLWFTGLSGAGKSTIAQIVEH
                     QLAERGQRVEVLDGDVVRTNLSKGLGFSKEDRDTNVRRIAFVADLLSRNGVVAITAAI
                     SPYREIRDEARGTMGERFVEIYVKASVDECARRDVKGLYEKAFKGEIKEFTGVSDPYE
                     EPSAPEIVLDTENETPEESAGKVLAFVEERLGVPA"
     gene            complement(36112..36744)
                     /locus_tag="VF517_16950"
     CDS             complement(36112..36744)
                     /locus_tag="VF517_16950"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX8054678.1"
                     /translation="MTTATAPDLEHASAEGVLRYAVEQYHPRLVLASSFQKESSVITD
                     MLLKIEPSTRIVTIDTGVLFEETHATRRAIEERYGIEIEVYDASAPDGLPWTGGERCC
                     GDFKVAALRSALGDAEAWVTGLRREHSPERASTGKVHWDERNQLWKVAPLADWSERDV
                     WRYIAENDVPYNKLHDQGYDSIGCVPCTQPGHGREGRWAGSAKSECGLHG"
     gene            complement(36741..38486)
                     /locus_tag="VF517_16955"
     CDS             complement(36741..38486)
                     /locus_tag="VF517_16955"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX8054679.1"
                     /translation="MIPTIEKEFDDFDTQAGRFLRGEVQEDQFIGFRLKQGVYGQRQA
                     NVQMIRVKLPFGGVSPDQMEAFAEVAERFTPLVKGHITTRQNIQYHHVPLPDAAKAIR
                     LLSEAGLSSREACGNTVRNVTGDPWAGITDELYDITPYAGAFVRYFVRNEVCQLMPRK
                     FKVAFTANDDDRAITKIHDLGFIPRVREIDGREVKGVEINTGGGTSIMARVGSTMYDF
                     VEVENGDYLKVAEAVMRIFNRQDWLRANRQRARLKFLVDKIGIDAFREQVDEELKGDW
                     VRERNFDPTLLLFQHDEEANAPLPPAVVASPNGDRSEFDAFRASNVRPQRQEGFVSVE
                     VKVTRGDLTPEMFRGLAAIMREYCGGYARTTVHQNLVLRWVREEATYDVYRRLVELGL
                     GDAGADQVTDVLSCPGTDSCKMGITSSMGLNRAIQDRVEGMGIEDPLTKRIHIKMSGC
                     PNGCGQHHLGTIGFYGASMKMGGKQVPAYIAHVGGRYEGGELAYGQRVKVRLPAKRVP
                     DAVERWIRLYERERQEGEEFNAFVDRVGTEPFEKEAKELSLPVEFNLENIQHFIDWSR
                     HEPYQVIRGEGECAV"
     gene            complement(38638..39000)
                     /locus_tag="VF517_16960"
     CDS             complement(38638..39000)
                     /locus_tag="VF517_16960"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8054680.1"
                     /translation="MTEETTLQLDPARARELIDAGAQVVDVRTAGEHAESHISGAAHI
                     PLDRLDKESAEALSADDPVLVYCRGGNRSEMAAEALRNSGFDAHSIDGGLVAWDEAGL
                     PLEPEGAAVAPPQNLPPR"
     gene            complement(39019..39207)
                     /locus_tag="VF517_16965"
     CDS             complement(39019..39207)
                     /locus_tag="VF517_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054681.1"
                     /translation="MAGPEEAAAIAAAIEQFLRDTAPAPAPTDEPADPWLRAALFEGV
                     GLAADAPSPWGDANPWDR"
     gene            39787..40725
                     /locus_tag="VF517_16970"
     CDS             39787..40725
                     /locus_tag="VF517_16970"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX8054682.1"
                     /translation="MPEIPVYDHATVLAAISAADAVERVRDGFVQFAHGDWEMPPKVY
                     VDAPPRGDFRAMPARGGGHAILKWVTSFTGNPAHGLPVVQGAILVSSAETGTPLALVD
                     VGAVTSLRTGAVAAVAAQELAREDARTVGIIGCGVNGSWAGRCLAAAEYGPGVCFDPD
                     PDRAGALAGELGWEAGSREDALGCDVVTCITPGHEPVVHATDLRPGMHLSMLGADAAG
                     KAEADVDAVTRCVLFCDEWRQASHGGELTQAVEQGLVARDDVTDLGAVITGTAPGRPD
                     DDAITLFDSTGLAIQDLALCLALVRDPPPATQTIRL"
     gene            complement(40747..41235)
                     /locus_tag="VF517_16975"
     CDS             complement(40747..41235)
                     /locus_tag="VF517_16975"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8054683.1"
                     /translation="MAKIVGPNDGDRVDLGGLGVRFMIDGETAGGGFSLVEHPIAPRT
                     LAAPLHRHVNEDEYSYVLEGRMGAQLGDETVEAGPGELVFKPRNQWHAFWNPGDEPAR
                     VLEIISPAGFERYFEEIAPLLPPNRPEPDLATLLQVAARYGLEMDPESIGPLMEKHGL
                     TG"
     gene            41273..41881
                     /locus_tag="VF517_16980"
     CDS             41273..41881
                     /locus_tag="VF517_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054684.1"
                     /translation="MARRVQQPRAYRKNVRAEHEAATRRRITEATVALHREVGPARTT
                     ISAVAERAGVQRLTVYRHFPDEAALFGACSSHWLTAHPIPDPAPWARVADPEERLRAA
                     LGALYEWYRGDEAMIANVGRDAPGIPALAAIVANGLPAYLEAVHELLMPGWGARGRRR
                     RMLSAALGHALDFETWRSLERRQGLEPENAIELMVRAVRAAF"
     gene            complement(41878..42576)
                     /locus_tag="VF517_16985"
     CDS             complement(41878..42576)
                     /locus_tag="VF517_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8054685.1"
                     /translation="MGAPQTRTSVVIPCLDEAAAIADCVRVARGAAGPGGEVIVADNG
                     STDGSAELAAAAGARVVSEPRRGYGRAYLAGLAAARGELIVMGDGDLSYDFGEIPRFV
                     RELEDGADLVLGNRMGAIAPGAMPVLNRYLGNPLTTGLVRALFRPGIGDVWCGLRAIR
                     RDALERLRLRSGGMEFALEMVVRAAQEGLDIRELDIELHPRGGESKLAPLRDGMRGVS
                     FLLAARAEPLRRSR"
     gene            complement(42589..>43747)
                     /locus_tag="VF517_16990"
     CDS             complement(42589..>43747)
                     /locus_tag="VF517_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054686.1"
                     /translation="LKALSLVSYGALGVAAYVLGARLFSRAVGVLFAALLLTRPSLVN
                     QALIASIDVPFLALIVAAAAIEVRRPWAGVPVLILLGVAGLIRPEAWLITGVYTLLLL
                     RDARGRERAKLIALAALPPLAWIASDLVITGDALWSFHQARATSERLADAERYGDGGP
                     AATIEWAARSWKGILHLVPALVATAGLAIVAWLWRRRAQVPLALLALGTASFLVIGFA
                     GLPLLTRYFFMPATMLCLLAAAGTLAWRELPEASRERGVARVAGLAMGVALLVHVPFL
                     VDHMSGALEKVDRAQRLQDDLRAIAEEPRVREQIPRCQPVQTRLFRARPTLLWARRDD
                     PPARIVATRRLELSAGMLLIYSLEERPLRPPGFERVAANRTWTVLARCPRG"
BASE COUNT         6164 a        15692 c        15408 g         6483 t
ORIGIN      
        1 cgtcgccgaa gcgctgcgtg tccaggagtg ctcccccgtg gcgcacgagg ctcgccccgc
       61 ctccgaagcc gaacgaggtc gagcaggcag gcaggttgct caactcgatt ggcggaggac
      121 agtcggacgg ctcgtagcgc gcgcagtcgt cgtacgagag gtcggcgttg ccggactcct
      181 cctcggcgtc cgatagcagc aggtcgccaa agcgcgggcc gagccaccag atcggcacgt
      241 ccctgtagga ggccagctcg ctcgtgtcgg tctcgatcag cggctcgcgc ggcgtcgcgc
      301 cgcacgcgcc cggatcgctg gagagccccg cacccggctc gaagtcaggc gcgaaggggg
      361 aggccgtgcc gttggtgagc caggtcaggc acgagccgtc ggcgcccatc gagtacacct
      421 cgaggctccc gccgcgcggg tagttgcggt cgctgacaaa ggcgatccgg tcgccgctcg
      481 gcgcccaggt cggcgtggac tcgcgcgctt cgttcgtggt caggcgccgg cgcccgccgc
      541 cgtccgcgtt cgcgacgtag agctcctcac aggcgtcctc ctcgcaccgc gggcgcacgc
      601 cgacgtagac gatccggtcc ccggccggag accagcggcc ctccccggcc ctgcgcagca
      661 gccgcctgcg cgtgcctttc gccgcgtcga ccacgtgcag ctcgggcggg tcggacgact
      721 cgcccgacag gtcccccacc gtcacgagga tccgcgcgcc gtcgggcgac cagtccgccg
      781 cgagcatgcc gacgtagcgc gacgaggcga tccgcgcgac gcggtgctcg tcgccgccgc
      841 cggccgccgc gctgaccagg gtggtcacga tcctctcctc gccgtagcgc acgcgcacga
      901 aggcaacgcg ccggccgtcc ggcgaccagg tgggcgatag ctcgagaccc cccgtcgtcg
      961 gcgcggcggt caggggccgg gcgccaccgc cgtcgggcgc gctgagccag acgcgactgc
     1021 cctcggagtc ctcgtcgacc tcgaagcgcg tccaggcgag cgtgtcgccg tccggggacc
     1081 aggccgcctc gcccgcgccc tcggcggcga cgaggcggcg gtccgagccg tcggcggcga
     1141 tcgtctggat ccggccgtcc gcgctgtaga cgatgcgcgc caggcgcggc tcctgcgccg
     1201 gcgccaccgg cgccgcggcg gcgagcgcca gcagggtcag cgcgccggag aggagcctca
     1261 gcatcccagt cgtctcctca cgccgagctg cgcgagccgc cggcccacgg ccaggcggcg
     1321 cttcacctcg ctgcgcttca ggcgcagccg tcgcgagaca gcgctcaggc tcctggtccg
     1381 ccggtacgcg ttggccacgc tcgtgagcag gcgccagtag cgattcggca gcctcgcggc
     1441 ggggaacggc gcgcccgccg cgcgctcgcc accgacggga cgcagatggt cgaggacggc
     1501 gggtggaacg gcgcggccgc tctcggtgat cccctccaca cgcgtccggt gcgcgtagac
     1561 gtgtgaccag ctgagctccg tgtcgcgctc cgtgtacagg agcgcgccgt ggaaggtttc
     1621 gatgccgccg ggacgcgcgc cgccgaggat gcgtcccgcg aagcagaggt cgtcgttcat
     1681 cagcagcagg aactcgccgc actcgtccgg gtcgaaacga ccgcagtcgg agtaggcgac
     1741 ctccacggag ctcttgctcg cctgcacgtc ggtgacgagc agcccgttag gcgcgacggc
     1801 accgagccac cagacccgga acgggcgcgg cgagtcgatc gcgcgcatgt cggtctcgac
     1861 cagcggttcg cgcgcgctcg ggccgcaccc gccgggatcg ccgctgagac cggtgccggg
     1921 ctgaaccgac ggtgcgtaac tgtcggccgt tccgttggtg agccacgtga ggcagctccc
     1981 gtcaggccgg atcgagtaga tctcgcgacc cgcttcctcg gtctccgcct gcacggcgtt
     2041 gcgatcgctt gcgaagacga tccgctggcc atcgccggac caggacgggt cgtcgtcgtc
     2101 ggcgcggctc tccgtcagcc gcacgcggtt cgagccatct gcgctcgcga cgtagatctc
     2161 gcccgcgacg atgcactcct cacggcagct ccgaccgagc cggtcgtgca taccggagta
     2221 ggcgattcgg ccgccgtcgg gtgaccaagc tccgtgcgcc gcatcgcgta tcagcaggcg
     2281 cgcctcgccc ccggcggcgg ggacggcgta caccgacggg cggtagtcct cctcgtcacg
     2341 gccgccgaag gtgctgcgcg tgaacaggac cgtctcaccg ttgggcgacc acgcgggcgt
     2401 ctgcaggtac ggtgccccct cttcgtcact gctctcgtag gtctcgctgt agaccgtgcg
     2461 acgctgcccg tcgtcgaggt tcacggcgat gatcgacgcg acgacgcggg tgcggccctc
     2521 gcgcacctgg acgaacgcga gctggcggcc gtcgggggac caggcggggt cgtgctccca
     2581 acgcacggtg ctcgccttcg cggtgatggg acgagtgccg gtgccatccg cacggatgac
     2641 gtgcacgcgc aggtcctcgg catcctcgtg atcggcggcg agctgggacc tgacgaaggc
     2701 gatcgactcc ccgttcggcg accaggcggc attgctgtct ccgccctcgt cgctgcgcgt
     2761 ggtcagctgc ctcatgtccg agccgtcggc cgcgagggtg cgcaggccgt agccggcgtt
     2821 gaagacgatc cgcgccttcg ccggagtggt catcgggctc ggctcggtcg gagtggccgg
     2881 aagctgcgcg gaagcggtcg cgacggtggc gagcgcgatc agcacgacga ccaccccgcc
     2941 cgctcgactc cccctgccca cccggtcagg ctagcgtcaa cgtgccaggc cgcgcgccgc
     3001 tcagaggtcc gcgaggcgcc ggccggcgct gcgcaggcgg cgcttcgtcc cggcctcggt
     3061 gatcgtcgcg aggtcgatca gccgagcctc gaacggcgcg agctcgttgc gcccggccac
     3121 cgcgtggcag gccaccccgg cctggcggca gcgggtgccc acctcgaaga cgaccttccc
     3181 cgcgagcgtc tggtggtcga tgctgccctc gcccgtaacg acgaaacgcg ccgcgcgcat
     3241 ggcgtcgtcg aagccgatcg tgtcgagcac gaaggtggcg cccggaacga ggctcgcgcc
     3301 gaggtgggcc cagagccccc cggcgagccc gccggcggcg ccggtcatcg gcacgccgcg
     3361 cgggtcgcgc ggcgcggcct tcgcgagccg agcgaggcgg cgctcgaggc gcttcacggt
     3421 ctccgggtcg gcgcccttct gcggcgcgaa cacgcgcggc gcgagctccc acggcgtgcg
     3481 cacgtcgcac acgaccgtga gcttcggcgc ggcccccgcg tcgtccagcg cctcgagggc
     3541 tccggcgccg ccgtcggtgg tcgcggagcc gccggcggtc acgatcacct cgcgcgctcc
     3601 gtccgcggcc gccgccgcga tcagctcgcc cgtgccgcgg gtggacgcgg caaaggcgtc
     3661 gcgctcgccc tcggccacga ggccgaggcc gctcgcgcgg gccatctcca cgatcgcgcg
     3721 ctctccgccg tcgatcagcg cgtactccgc ctccagcggc cggccgagcg gatcgctcac
     3781 gcgcgcgcgc cgccgctcgc cgccgagcgt cgccagcagt gcgtccatgg tcccctcgcc
     3841 gccgtccccg accggtagct cgaccgcctc caccccgccg tcccgcagcc ccgccgcgac
     3901 cgcggcggcc acctcggaag ccgccagcgt ccccttgaag gagtccggcg cgacgaggat
     3961 cgagttcacc cggcgtcgac cgctgcccgg aacgcgcgct cggcttcgtc cccgtgcacg
     4021 gcgaacacga cccgctgcag ggaccgcgcc tcccaccggc gcaccgcacc gaccatgatg
     4081 cgcgccgcgt cgccgaccgg gaagcccccg actccggtcc cgaacgccac gagcgccagc
     4141 gagcggcagc cgagctcgtc ggccttcgcc agcgtcgagt cggtcgcgcg ctcgatgatc
     4201 tccgccgacg tcgggccgcc gagctccatc gtcgccgcgt ggatcaccca gcgcgcgggc
     4261 aactcaccgc cgctggtctc gaccgcctcg ccgagcccga tcggcgcccg ctcgcgcgac
     4321 tcgcgctcga gctccggccc cgccgcccgg gcgatcgccg ccgccacgcc accgccgtgc
     4381 cgcaggcgcg tgttcgccgc gttggcgatc gcgtcgacag ccagcttcgt gacgtcgccc
     4441 tggatgacct cgagctcggg cacgctcagg cgcgcagctg ctccatcgtg cactcctcgg
     4501 ggctcttgtc gtcgcgccag cggtggaact tcgccccgtg ccggatccgg ttgccggtga
     4561 tgtggtcgaa cccgatctca cagacgagct ccggtcgcag cccctcccac acgagctcct
     4621 cgtcggactt ccagcggctc gcctcgccgg cgccgcgctc gtaggtgcgg tagggctcca
     4681 ggaggtcgat cagctcgcgc ttctccttcg ccttgaaccc cgacgtgtgg ccgaccacgt
     4741 ggagctgccc gtcctcgtcg tagaggctca ggatcagcga gccgaccgtg ttcggctcct
     4801 tgccgaagcg gaacgcctgc acgaccacgt cggctgtgcg gactcgcttg atcttccgca
     4861 tcccggtgcg ctcacccggc aggtagggcg cgtcggccac cttcgcgatc acgccctcgg
     4921 actggcctgt gagccagatg cgcgcggcct ccgcgtcgtc ggtcgccggc gtgagctgga
     4981 cctcggagcc cgcgaccgcc tcggccagcc gctcgcggcg cgtggtgtat gggagctcga
     5041 gcagcgtctc gtcgccgatc gcgaggagat cgaaggccac gaacccggcc ggcgtctgct
     5101 ccgcgagcat ctcgacccgc gacttggcgg ggtggatgcg ctgcccgagc aggtcgaact
     5161 cctggatccc gtcgaccacg acgatcagct ccccgtcgag cactatccgc tcgggggaga
     5221 gcgacagcac ctgcgggacg atctccggga agtagcggtt catcggcttg ccgttgcggc
     5281 tctggaggtg cacttcgtcg ccgtcgcgga agacgacggt gcggaagccg tcccacttcg
     5341 gctcgaagaa ccagccgtcg ccggcgggca gatccttcgc ggggcgggcc agctgcggct
     5401 tgatgggcgg cttcagcggg agggacatcg ggtgagggat agcatggccg gggatgtcgt
     5461 cccagcaggc aaccgtcgcg gaggaggagt atctccagac gatctactgg ctgttcgagg
     5521 ccggactgcc gatgaccgcg gcgaacgtcg tccgcgccat gcaggtctcg gcgccgaccg
     5581 tgcacgagat ggtcggccgc ctcgagcgcg acggctacat cgtgcgcggc gacgaccgct
     5641 cgctcgcctt cacggacgag ggccgcgagc acgccgccgg cgcggtccgc cgccaccggc
     5701 tgatcgagcg cttcctcacc gacgtgctcg ggatcccctg ggacgaggtg cacgaggagg
     5761 ccgagcggct cgagcacgcg atgtcgccgg tgctcgagga gcggatgctc gccgcgatcg
     5821 gcgacgcgcg cacctgcccg cacgggcacc cgatcgaggt cggggcgcgg ctgccgggcg
     5881 tgccgctggc ggatgtcgag gagggtgctc gcgtgcgcgt gctgcgcttc gagaacgagg
     5941 ccgaggacct gctgcactac ctcaagggga ccgggctgtt cccgggcctg gaggggacgg
     6001 tcgccgtctc ggacgacgag gaggtggtgg tcgaggccgg cggcgagcgc cgcgtgctca
     6061 cccgcagcgt ggccgagacc gtgaccgtca cggccgaccc ctcgccgccg ccgcgcgtgg
     6121 cactgcccga gcagctcgtg ctcgcgcgcg agcgctacgg gcgctagcgc agccgcgcgc
     6181 gacacggcaa tgcgccagaa ccccttttgg gcggggaccc cctacccgtc cgggacccga
     6241 gcggttagtt tgggcggcat gacccgcctg gcccgcgccg tctgctgctc cctgctgctc
     6301 ctgttcgtcc tcgcgccggc gagccacgcc gcgatcgcgt tcgagccgtg tggggaacgg
     6361 accgagctcc agtgcggcac cctgacggtc ccgatcgacc ggtccggctc ggtgccgggc
     6421 acgatcaagc tccaggtcac ccgcgtcccc gccgtcaagc cgagcggcaa ggccgtgttc
     6481 gcgttcgccg gcggcccggg tcagggcgcc accccgttcg ccgacagctt cgcggcccag
     6541 ctcggccgcg cgcgccgcac ccacgacatc gtcgtcttcg accagcgcgg caccggcgcc
     6601 tccgcgccga tcgactgcac cgacgtcgac cgcgtgcgcg acatccgcga cgccgccgcg
     6661 cagatccgcg cctgcggcga gaagctcggc ccaaccgcgc cgttctacac gaccgcggac
     6721 accgtccagg acatcgagtc gatccgcgcc gagctcggct acgagaagat cgggctgttc
     6781 ggcgtctcat acgggaccaa ggtcgagctg gcctacgcga gcacgttccc gcagcgggtg
     6841 tcgcggatgg tgctcgactc cgtggtccag gccgacgggc ccgacccgct gctgcgcgac
     6901 agcttccgcg ccgtgccgcg agtgctgcgc gagctgtgcc gcagcgccta ctgccgcggc
     6961 gtcacgaccg accccgtcgc cgacatggcc gcggccgtcg cacgcatgtc cggcgggccg
     7021 ctcaccggca gcacctacag cgtgaagggc aagcgcgagg cggcgtcgat cagcagctcg
     7081 acgctgttca cgctgctcct gatcggggac ttcgagacgg ccctgcgggg cgctatcccc
     7141 tcggcggtgc gctcggccgc gctgggcgat cccgcgccgc tgctgcgcat ggccaagctg
     7201 atcgagcccc cgccgaatcc gcaggcgccg gagctgagcg agttcagcac gggcctctgg
     7261 ctggcgacgg tctgtgccga gctgccgttc ccgtgggatc ccgacgcacc ggagtccgag
     7321 cgccgcgcgc aggcccagca acgctcggcc gcgctcgacc ccgcgcagtt cgacccgttc
     7381 gacccgggca cggcgctgtc ctgggacatc gtcgagatgt gccagcactg gccggtggcg
     7441 cgtcgcccgg cgacgggcga cctcagcaag gtggaggcgc cggtgctgct ggtcaacggc
     7501 cgcatcgacc tgcgcacccc gctcgagaac gccgaacgga tggcggccct gctcccgaac
     7561 gccaccgtgc tgccgatccc cgaggtcggc cactcggtgt ccgacgccga cccgaccggc
     7621 tgcgccgggc tggcgaccga cgacttcctc gccggccgca cgctcgtgcc gtgccagaac
     7681 gcgcccccgc ggatgcggat ccgtccgttc gcaccgaccg acctgaacgc gctgtcgccc
     7741 tcgaagggca cgtccggaaa gcgcggtcgc acggccaagg cggtcgacct caccatccac
     7801 gacgtgatcg ccgagctcga cctcttctac gccgaccgcg gcggcctgcg cggcggctcg
     7861 ttcacgctcg gcgacgagga catcgtgttc cgcggcctcg tgtacgtgcc cggggtgaag
     7921 gtgagcggcc ggctgaacgc gttcagctac gggggcacgc tgcggatctc cggctcgaag
     7981 gccgcgcgcg gcacgctgcg ggtgggccgc aagggcgccg tgaccggcgt gctcgacggg
     8041 cgcaggatca gcacccggat caagctgccg aagccgcccg agggcctcgg cggcggcggg
     8101 ggcgaagagg gtccgggcga gccgcgggtg ccgacctggc tcggcccgct gctcggtcgc
     8161 tagcggaggc gggtcgccgc cctaggcggt gacgctgagg ttcttgtagg cgtcggccag
     8221 ccggccgatg cccgcgcgga tctggtccgg cccgacgccg gagtacgcca ggcggagcgt
     8281 gttgaggccg ccctcgatca ggaagtcggt gcccttgacg aagatcacgc cgcggtcctt
     8341 ggcggcggcg gcgagggcgt cgacgtcggc gccctcgggc agctcgaccc acaggaagta
     8401 gccgccctcg gggggcgtga agcgcgcgtc cggcagctcg ctctgaagcg tctcggcgag
     8461 ggcgtcacgg cgctcgcgga gcgcgttctt gacggtctcg accgagcggt cgatggcacc
     8521 ggaggtgcag aactcggcga cgatcgactg cgacaccatg ctcggggaga tgtaggtgct
     8581 ggtggcccgg cgctcgatgt caccgatcac ggcgggcggc cccgcgaggt acccgacccg
     8641 gatcccggga cagaccgtct tcgagaacga ggacgcgtag accacgcgct cggcgtcgtc
     8701 gaggtcgaac atcgtcggca gcgactcgcc ctcgaagcgc agctccccgt agggatcgtc
     8761 ctcgaagatc atgaagtcgt agtggccggc cagctcgagc aggcggcggc gcttctccag
     8821 cgagagcgtg acgccggcgg ggttgtggaa gttggggatg acgtgggcga gcttcggccg
     8881 cttgccggcc tcgagctcgc gggcgaggta ctcgacgtcg aggccgtcgg actcgacggg
     8941 caccgggagg agatcggccc ccatgtttcg caggacgagc agggtgcggt cgtaggtcgg
     9001 cgcctcgatg accacggtgt cgccggactt gacctcgcgc tggaacagga acgcgtcggc
     9061 ctgcatcgag ccgttggtgg cgatcacctg ctcgcgagcg atctggtgct tctgggcgag
     9121 ccagtcgcgc agcgccgggt agccgccgga ggtgccgtac atgaacgcgc ccgaggggtc
     9181 gttctcgaac gcgcgctgcg cggcctgctt gaggtcctcg accgcgacga tgtcgagcga
     9241 cggagcgccg cgcgcgaacg tgatgggcgc cgttgactcc acgcggatga agataacgca
     9301 cgcctcgcga ccgattagtt tccgccggcc ccccggtctt gacatgcact gagaacattc
     9361 gcgcggacct tccgccgcaa cggagaggat gcagccatgg agcagaagat cgtccccaac
     9421 ctgtggttcg acggccaggc cgaggaggcc gcgaacttct acatctccgt cttcaaggac
     9481 gggcgcatcc tgagcaaggc gcactacacc gaggactcgc cgggcgagcc cggcacggtg
     9541 atggtcgtcg agtgggagct gaacggtcag cgcttcgtcg gcatcaacgg cggctcgcag
     9601 ttcaagttcg acgaggccgt ctcgtttcag atctcctgcg aggaccagga ggagatcgac
     9661 tactactggg acaagctgtc cgagggtggc tccgagggcc cgtgcggctg gctgaaggac
     9721 aggtacggcc tgtcgtggca ggtcacgccg aagggcatgg acgagctgtt ctcgggccgg
     9781 gacgaggagc gcgccaaccg cgcgatgaag gcgatgctga agatgtcgaa gctcgacatc
     9841 gccgagctgc gcgcggcggc cgacggggtg ccggcggcct aacggcgcga ccgcaggcgg
     9901 tggatctcga cctcgtcgcg gacgctcgtg gcgaccgacc agtggcggcg ggcctgcttg
     9961 acgaagggtt ccgccgccgg gcggccgggg tcggccagcc acgcctcgcc gccgaggcgg
    10021 gcgagcagcg actggagcgg agcggcgctg gcgcgctcgt agagcacgtc ggcggcgagc
    10081 accaggtcga acgggccgag cgctacgagg gagtcggccg cggcccactc gacgcggagg
    10141 gtggtcaggt cgaggtcgtt ctcggccgcg ttctgccgga cgagctcgag cgcctcggcg
    10201 tccgcgtcgg tggcgagcac cgtggcccca gcacgagccg cgacgaggct cggcaccccc
    10261 agcccgcagc cgagctcgac cacgcgcagg ccctcgagct cggccccctc gagctcgcgc
    10321 gcgagcgcca ggccgctgcg ccacagcacc gaccagtacg ggagcagcgg agcccactcg
    10381 accgggccgt cgtcgggcag ctcggcggcc tcgtagggct ggaggatcgt cagctcgccg
    10441 cccgggagcg ggatgcggtg acggacgagg tctgagccca ctgccatggg cggcgaagat
    10501 aacgggggcg tgctgtcctc ttccccgtgc ggcgttcgtc tacaggcgac catcccgaca
    10561 aggaggcccc atgaactaca tgctcctgat ccaccaaggc gacactccga cgccgcagac
    10621 gccggacgag tgggcgaagc tctccgagga cgagcgcaac gccgtctacg cggactacaa
    10681 ggcgatcaac gagaccccgg gcgtgactcc cgggctcggt ctccagccgg ccgacacggc
    10741 caccaccgtg cgcgtggcgg acggcgagac gctgaccacc gacggaccgt tcgtcaccgt
    10801 caaggaggcg ctcggcggct ggctgatgta cgaggccgac gacctcgacg ccgcgatcga
    10861 gctggcggcc cgcatcccgg cggcccgcat gggcggcgcg gtggaggtac gtccgaccca
    10921 ggagtggtag cgatcgccct cgaggacgtc ttccgcgacg agtggggtcg cgtcctcgcc
    10981 agcctgatcg gtttcctcgg cgacttcgag ctcgccgagg aggccgctca ggaggctttc
    11041 gcaatcgccg ccgagcgctg gcccacggcc ggcacgcccg ccaacccgcg cgcgtggctc
    11101 gtggcgacgg cgcgcaaccg cgcgatcgac cgcatccgcc gcgaacgcgt gggcgcggag
    11161 aaggcacgac tgctcgagac ggtgccccaa gccatggacg accccttcgc acacgacgac
    11221 acgacgatcc gcgacgagcg gctcgagctg atcttcacct gctgccaccc ggcgctggcg
    11281 gtcgaggcgc aggtcgccct gacgctgcgg agcctgggcg ggctgcggac cgaggagatc
    11341 gcccgcgcgt tcctcgtgcc ggaggcgacg atgaccaagc ggctggtgcg cgcgcggcgc
    11401 aagatcaggg acgcggggat cccgtttcgc gtgccggccg accatctgct gcccgagcgg
    11461 ctcgccgcgg tgctcgcggt cgtgtacctg atcttcaacg agggctacgg cgggcgcggg
    11521 gagctggcga ccgaggcgat caggctcggc ggtgcgctgg ccgagctgat gccggacgag
    11581 cccgaggcgc atgggctgct cgcgctgatg ctgctgaacg acgcccggcg cgatgcgcgc
    11641 ttcgccggcg gggcggtggt gctgctcgcg gaccaggacc ggtcgctgtg ggacgcccgg
    11701 cagatcgagc tcggccgcac cgcgctcgat cgcgcggtcg ccctgagtgg gagcggtccg
    11761 tacgtggtgc aggccgcgat cgccgcgctg cacgtggacg agcaccccga ctgggcgaag
    11821 atcgcggcgc tgtacggcac gctcgcgcag atgaccggct cgccggtcgt ggagctgaac
    11881 cgcgccgcgg ccatcgccga ggccggtgac gcggaggccg cgctcgcgct ggtggagggg
    11941 ctcgacctgg acggctacca ctacctccac acgacacgcg gggagctgct gtgccggctc
    12001 ggacgcgtcg aggaggcggg cgtcgcttac cgccgcgcgc tggacctggt tcactcggac
    12061 gcggagcggc gcttcattgc gggccggctc gccgagctat gacctacgcg cttgagccaa
    12121 tagggcgggt ggaatccccg ctgacggatc tcgcctccgc gccgaggcag ggcgacgagg
    12181 gcgcgccgga cgcgtggctg gtcttcgagc cggccgtgct cgacgcgctc gacggggtcg
    12241 gcgtgggaga ccacgtgatc gtgctgacct ggctcgaccg agcggaccgc tcggtgctgc
    12301 gggtgcaccc gcgcggcgat ccctcgcggg cggagcaggg ggtgttcggc acgcgctcgc
    12361 cagaccgtcc gaacccgatc gggctgcacc gggtggaggt gctgtcgatc gagggcgcgc
    12421 gcgtacgagt gagtgggctc gaggcgctcg acgggacgcc gatcgtggac ctgaagccgg
    12481 tgctgggcag cgacccgggt gagcgctgag ccgatccgcg gcggtcaagt cacgtttgcg
    12541 tgccgcccgc tccagactcc agcgaagcga catagtcagc aagctgatcg atcgtgtggc
    12601 gcatcttgcg tattgccgcc tggctgtctg tctcggtgaa cttgccaccc caaagctcgc
    12661 cattcaggaa gatgctggcg atgttggtgt cgggccgggc gcgcgcgagc acgtagtgca
    12721 gacgcgtgag gagctgctca gcctgggccg ccgtcccttc ccagcggccg ttgtccacct
    12781 cgatgacgac ccgatcgcct acttcggtga tgttgatgcc ctcgacgggc cgctccgtca
    12841 tcggccctcc ttccgcacgt tcatccgaat acggagtgtg cctgcgacat gggcgccgga
    12901 atgggcagcc cggcttcacg cataccttcg agatggaact cgacggcttc gtcgatcagg
    12961 gaacggactt cttgttcaga ccccgccgcc gcgataaccc ccggaagatc aggcacgtat
    13021 gcgctccatc cggtgtcacc gccctcgatc acgacgagat actcgttccg cagcacgcga
    13081 tccggctcgc gcaccaacgg aaactcggtg cctggctcta cggacaaagc gctcatcgca
    13141 actcctccaa ttgggcctgt ctgcggatgc ttgccaacgt tccagggcgt acttcgtcgc
    13201 tccgatttcc cgaaaccgtt accaggccct tcttgtgcgg gtgcttgtac tgccggtggc
    13261 tgcccctcgt tcgatcgagc cgccatccgt ccttttcgat cgctcgcatg acatcagcca
    13321 ccttcacgca gttacggtaa acgaagatcg caggggcggc tccggccgcc actgcgttgg
    13381 ctccggccga cgccgtcttt agcatcgccc acgcgatgcc cagcgtgcgc gcatcacccc
    13441 tgccgtcgcg cccgctcgcg atcgttcccg agcggctcga gagctgcgtg cggcggctcg
    13501 atccggctga ccgggcgctg ctcgacctgt ccctgaaccg cgggatcccc gacgccgcca
    13561 tggcgccgat cctgcgcacc gatccgctgc gtctcgcgtg gaagcgtgcg cgcgcgatcg
    13621 agcgcgtggc ctcgcgcctc gggctgacgc atcccgcgga cctcgggctc gtgcgcaccg
    13681 cgctggccga tctcccctcc cgcgcctggc tccccctgga gctccagccg gcgaagcagc
    13741 cccgcgtgct ggagcccccg gagccggtgg gcgaggagcc gcgagtgatg aagccgagcg
    13801 tgcagcccgc ggcgacagtc gcgccgccgg aaccggccgg cgcggagccg cgggttacaa
    13861 ggccggctgc ggacgaaagg cccgggggtg gcaccgcgcc agcgacgccg cccgagccgc
    13921 cgggcgcgga gcccgtcgtg ttccgcgcgc gcgagccgga ggccgtgcgc gcgagcctgc
    13981 gcgcggcggc gcagccgggc accgcgctcg cacccgcgcg ccccgctcag gccgcggcag
    14041 gactcgtcgc gaagcccgtg agcacttacg gaagagggct cgccacgccg gcccgagccg
    14101 aagacagcgg cgtccagttc gggcgtgcgg cgtcgttcac actcgggggg ctctctgagc
    14161 gagcgaagcg agcgtacccc cgagggggaa tgacgacgcg cgcccgccgc gcggccgggg
    14221 ccctggcagt tggagccgcc gcgctcctag cgctcaggcg gtcgtagccg cagccacttc
    14281 ggcggccagc tcggccacct gcgtgggcgt acgaccgacg cgaatccccg cggcctccag
    14341 ggcctcggcc ttcgcggccg ccgtcccctt cgaccccgag atgatcgccc cggcgtgccc
    14401 catcgtcttg cccgggggcg ccgtgaatcc cgcgatgtag gcgagcaccg gcttcgagac
    14461 gttctcgcgc acgtactcgg ccgtgcgttc ctcctcctcg cccccgatct cgccgcacat
    14521 gacgatcagc tcggtctcgg ggtccgcctc gaagagcgtg atgatctcga tgaacgacga
    14581 gcccgcgatc gggtcgccgc cgatcccgac gatcgacgag ttcccgaatc cgcgctgggc
    14641 cagctcgttg ccgatctggt aggtcagcgt gccggagcgc gacacgaggc cgacgttgcc
    14701 ctcggagaag aagtgcgccg ggatgatccc gacgttcgcc ttgcccggcg acaggatccc
    14761 cgggcagttc ggcccgacga ggcgcgtgga cccgcggcgc aggtgcgtgt acacccgcag
    14821 catgtcgtga acggggatcc cctcggtgat gcagatgacg agcccgatgc cggcgtcggc
    14881 ggcctccagg atcgaatccg cggcgaagcg cggcggcacg aagatcatcg cggtgttggc
    14941 gcccgcctcg tccaccgccg cgtgcatcgt gtcgaagatc ggcacgccct cgacgtcctg
    15001 gccgcccttg ccgggcgtca caccggcgac gaggtcggtg ccgtacgagc ggttgttcag
    15061 cccgtggaac gagccctcgc gcccggtcag gcccgcgacg acgagcttgg tctccttgtc
    15121 gacgaggatg ctcacgccgg agcccccgcc ttcgccagct ccacgacctt cgcggcagcc
    15181 ccgtccatcg tcttctcgac ggtcacgttc ggaagcgcgg cctccgcgag gatctcgcgg
    15241 ccctccacgt cgtttgtccc gtcgaggcgc accacgaacg gcaccttcac gtcgatctgc
    15301 ccgaacgcgg tcacgagccc gcgcgcgacc tcgtcgcagc gcgtgatccc gccgaagatg
    15361 ttgaacagca ccgcgcggac cttctcgtcg gagaggatta cctcgaccgc gtccacgatc
    15421 gcgtcggcct tcgagccgcc gccggcgtcg aggaagttcg cgggcttgcc gccggcctgc
    15481 gccaccacgt cgagcgtgga catcacgagc ccggcgccgt tgccgaggat cccgatgtcg
    15541 ccatcgagct tcacgtaggt gagcccgcgc tccttggcca tccgctcctg cgggtcctcg
    15601 tcggagacgt tccggaacgc cgccgtgtcc tcgtggcgga acagcgagtt gccgtccacg
    15661 gtgaccttcg cgtcgagcgc caccacctgg cggtcgcccg tcacgagcag cgggttgacc
    15721 tccatcagca ccgcgtcctc ggccacgaac gcgtcgtaga gcttcgcgag cagcgcgccc
    15781 accgggcgca ccacgtcgtc ggcgatcccg gcctcgaagg ccagccgccg cgcgtggaag
    15841 tccgagaagc ccacgagcgg gtcgatgtgc acccgcacga gcgccttcgg gtccttctcg
    15901 gcgacctcct cgacgtccat gccccccatc cgcgagagca tcgccagcgg cttcttcgcg
    15961 gtgcggtcga gcaggatcgc ggcgtagtac tcctcgtcga tgtcggatgc cttctcgacg
    16021 tagagctcgt gaacggtgaa tccgcggatg tccatcccga ggatcgcctt ggagtgctcc
    16081 tcggcctcgc cgcggtcctt ggccagcttg atgccgcccg ccttgccgcg cccgccgatc
    16141 agcacctggg ccttgatcac gaccggatat ccgagcgcct cggccgcctc gaccgcctcc
    16201 tcgacgctcg aagcgggctt tccatccggc gtcggaacgc cgtgctttcg gaagagctgc
    16261 ttgccctgat attcgaggag gtccatgaaa acctagaatg tggcccgccc cgcgcttcaa
    16321 aggccgattg cagggccacg gccggcgggc accctatcga aaaccgaacc gcttccccct
    16381 aggagacact tgccgatccc caaggacgtc cgcttcgtca cgttcgactg ctacggcacc
    16441 ctgatcgact gggagtccgg cgcatacgac gccttccaga aggaggccga ccgcgacggc
    16501 ttcacgatcg acaagaactc actggcgccg aagttcatag aggcgcagca cgagatcgag
    16561 cgcggctcgt acgagctcta cgccgaggtg ctgcgccgca ccgccgtgcg ctgcgccaag
    16621 gagctcgagt gggacctcga gccctcgcgc tcgggcttcc tcccgtcgag cgtcccctac
    16681 tggccgccgt tccgcgagac caatgcgcag ctcgagcgat tcgccaagaa gttcgagatc
    16741 gggatcctct cgaacgtcga cgacaagctc ctgggcgcga cccggcgcca cttccgcgtc
    16801 gacttcgacc tggtggtcac cgcgcagcag gtccgcagct acaagcccga cccggcgcac
    16861 ttcaaggagt gcgcgcggcg cgttggcagc aagtccggct gggtgcacat cgcgagcggc
    16921 tacgacaccg acatcgagcc gatcctgaag atgcgcgtgc ccgtgatctg ggtgaaccgc
    16981 cgcggggtca agctcgagcc ggggcagaag aagccgaccg aggaggtcaa gaccctccgc
    17041 gacgcggcca agctactgaa ggtcgcctga cctgagagcg gtagccgtcc accacgacgt
    17101 cgtggtggtc acgagccggc tctggcagac gaacgccacc gccatccggc gtggcgagga
    17161 gtgcctgctg atcgactcgc cgtacttccc cgacgagctc gagctgctgc cggcgctgct
    17221 cgcgcaggcg ggcttcgagc ccgacggcct gctcgcgacc cacgccgact acgaccacct
    17281 gctcgggcgc ctggcgttcc ccgggctggc cctcggcgtc ggctggccca ccgccgagcg
    17341 gctgcgctcg gagccgggcg cggcccagcg cgagctgcgc aatgccgacg cggagcacta
    17401 cgtggcccgc ccggcgccgc tggccctcgg ccagtaccag gagctgccgg tgcccgggtc
    17461 gatcgagatc ggcgacgcgg agctcgagct gcatgcgacc gacggccacg cgcccgacgg
    17521 caccgccttc gtggcccgct tcgccgggct gctcgtctgc ggcgactacc tctcggacgt
    17581 cgagatcccg ctcatcgcca gggccgggtc gctcgacgac tacagggcga ccctcgcccg
    17641 cctcacgccc ctggtcgagg cggctgagac gatcgtcccc ggccacggct cgccgcacga
    17701 ccgcgagacc gccctgcgca tcctcgacga ggacgccgac tacctggacg cgctggcggc
    17761 cggcgaggag cgtcccccac tccccgccag ccgcgatacc tcacgccagc gcgccatcca
    17821 tgcggacaac ctggccaagc acggcccggc tgcctagccc cccacggtca cccgcagggc
    17881 cggcgatccg tcgccgttca gcgcctggtc cccggcccac tgggccacga acgaggagct
    17941 gcgcttcacc gtccagttcg tgatgaacgt tccgtcggag cggaccgtga cgaactgcct
    18001 ggtccagcgg cccgacccga gctcgcgcac gctcacctgg acgcgggcgc ccggggccgc
    18061 gggagcgagc ttgccgtcca ggcgcacctt gcccttgcgg cgcaggcgct tcgtcttggt
    18121 cgtcagcgtg atcgtcgacc cggatgccgc gtcgccgccg ctcgccgtgc tgaacaggtc
    18181 ggacgtgccg accagcgcgt gtgcgatatc gcccggcgcc gccaccagcg agccgaggcc
    18241 cgaggtgatg ttgtcgttcg agatgagctg cggccgccag ctcgctccac cgtcggaggt
    18301 gcgcagcacc cacccgccgt tgaagttgcc gaagtcgcgg accgcgaggt agcccgtgtt
    18361 cttgtcgccg aaggccatct cgtaggcgtc cgcgctgccg accgtcagca gctccttcca
    18421 cttcccgccg cggttggccg tctgccagac gcggccgtcg gcggtgagca cgtagccgag
    18481 cttcttgtcg acgaagtcga cctcgaggat cgtgctccgg ctcggacgcc gcagggcggc
    18541 ccacttcgcg ccctcgtcgc cgctgaacgc cagggccttg ggcccgctga cgtacacgcc
    18601 gccgtcgggg gtgcgatcga cgtcgtcgag cccgaccttc gggccgaccg gcgccgcgtc
    18661 gaccgggtcg aagttcgcgc cgccgttggc ggagcggacc atcccgcgcg gaccgatcag
    18721 catgaccacg ttgccgtcac cggacgccgt gatcgaccgc gggcgaacac ccgcgtcgcc
    18781 aaggatcgcc cagctcgtgc cgccgttgtc ggtgcggaag accgcgccct ccgtgtcgac
    18841 cgcgtagccc agcgtggcgg tcgggaacgc gacgtccacc acgtcgttcg tggtcggcgc
    18901 acccacccgg gaccacgtgg cccccgcgtc ggtcgagcgc gccaccgagc cgcccgagcc
    18961 cggtgcgaag accgtgctct gcgaggtgag ccgcaggcgg ttgtaggcgc cctggagcgt
    19021 cgagccgatc ggcgtgaacg tgtcgcccgc gttgtcggag gtcgcggcct cgccgcgccg
    19081 gccgacgacc gccacgcgcg tcgccgagtt gtagctcgcc gccagccccg ggatcgggag
    19141 cgtgccgctc gggccacccg gaggcggctc gtaggtgctc ggctcgagcg tctcgaagcc
    19201 gtcgtcggtg tgcagcacga ggccgttgcc gccgatcacg acgcagtgca gctcgtccgc
    19261 gcagcgcacc gagcgcagcg gcgacgagac ggacgcgtcg gtcgtggtcc agttggcccc
    19321 gccgtcgacg gtcttcagca ccgcgttctg accgaccgcg tagccggtcg tcgcgctcac
    19381 gaagtacagg ccgcgcagcg caccggaggt ggccacgtcc ttccaggacg agccgccgtc
    19441 ggtggtgcgg tagatgcgcc cgagcgtgtc ggagccggtc acgaccagtc cgttgtccgg
    19501 gctcgtgaac cacgcgtcgt tcggcgggcc gcctccggtg gcgggaagcg ccgtgcgcga
    19561 ggcgaagcgt gcgccgccgt cctcggtgcg cagcaccgag ccgtcggagc ggatgaggaa
    19621 gccgatgtcc ttggtggcga agtgcatcgc ggccagcggc gagccgcagt tcgccgaggg
    19681 gttgaagcgc agccgccgca gcgtctgacc gctgtcgtcg gagcgcagca gcgtgcagcc
    19741 gccaccgacc aggaacgtgc tcgcgtcgat gacgcgcagc ttgctcaggt cgtcgacgag
    19801 accggtgcgc acgccgctcc aggtcgtgcc gccgtcgtcg gtgcggagca ccgtgccgaa
    19861 gtcgcccgcg gcgtagccgc gaccgccggc gaagtccacc gccttcagct cgttgccctg
    19921 cggacgtggg ttgccccagc tccagccgga ctggctggtc aggaccgggg cggccgacgc
    19981 cacggcgcag atggccatgg tgaaaacggc agccgcgatc gacgcgattg cgcagcgttg
    20041 catcatcctg cgagtcctct ccttcgaatt cccccggacg cgctcccgcg cgtcgcatgt
    20101 cgagagcgct aaaccgccgt agaggaagaa agttgcggag ccgcgcctac gagttgcggc
    20161 gaacgtgcca ccgcacgctc atcgaggcga ccacctggcc gtcctcgtcc ttcatctcga
    20221 tctcgaccgg gaactcgacc ttgccgtcgg actcgatctg ctcgagcagg ccgccggcgt
    20281 cgccgccgag cgtgccggtg gcggtgatcg ggcccttggc gagcttcctg tagtcgatct
    20341 tggcgctctt ggcgagcgcg gtcaggctgc ccatgtgctc ggcgaagacg ctcacgaacg
    20401 ccgcgcccga cgccgcctcg cccgccgaga acaacccgcc cgcgtgctgc gagccgacgt
    20461 ggttgtgcag ctcgccgtcg tccggcaggc gaaccacgcc gcgcccctcg gcgatctcga
    20521 ccacctcgag cccgaggtgc ttgttgaacg ggatcgcctc ctggaggccg gcgcgcatcg
    20581 cttcgtagtc ggtcacggtg gctgcaacct accggctgac ggtctcctgc ggctcggcga
    20641 cgcgcgcgat caggaccgcc gagaaggcca tgccgccgaa gaggaggaac ccgagcgtgc
    20701 cgaagtcgcc caccgcgagc acgagcccgc cgaacatcgc gccgatcgtg tagccgagct
    20761 gcgcgctggt ggtccgcgcg gccatcatcg cgccgggctg ggcgggcatc agccccaggg
    20821 cgagcgcgct cgagccggta gagcgcacgc cggcgcagag agccatgagg aagaacatgg
    20881 cgaccgtgaa ggccaccgct ggggtcacgt tcatgatcag cgcgaccagg ctgcccatcg
    20941 cgagcgcggc ggtcacgacc agccgccgcc ggcgcccgcc gagacggcgc acgagccggt
    21001 cggtgccgag cgtcgagatc agaaacgcca gcgagccgat cgccagcagc actccgacga
    21061 gcgcctcctg cacgtcgtag gtctccacgt agaaggcgcc gatgtaggtg agctccgcgg
    21121 tccaggcgga gtaggccacg aactcggcga tcgtccaggc ccgcgcggag gggtcgcggg
    21181 ccacgtccgt catcccgctg ggcgtcgact ccgtctgctc ctcgccgcgg cgcacccgcg
    21241 tgagcagcgc ggttgccagc gcggcgaggg cggccgcccc cggcaccgcg tacgcgagcc
    21301 gccaagacac cgcgtcggtg agcacgccga tgaccgggtt ccccacgatc caggcgatcg
    21361 actgcgatcc caccacgtag ccgagcgccc acggcgcgtc ctcctcgtcg aaccacgcgc
    21421 ccgcgcccgc gaagccgccc gagagcaggc acgcgacccc gagcccgatg aacgcgtgcc
    21481 ccaggtagaa gaccggcagc cccgccgccg tcgaggtgat cgcgtgaccg gtcacggcga
    21541 gcaccgcgcc ccacgtgatc accggccgca cgccgagccg gtcgatcagc ggtccgaccc
    21601 gcagcgacgc gacggccgcc accgccgcca tcaccgtccg ggcctgccca accgcgctca
    21661 ccgacgcgtc caggccgtgc ccgatctcga ccatgatcgg ggcgagcacc atgatgctcg
    21721 cctgcgcggc gagggtcgcc aggacgagcg ggatcagcgc gcgggggtcg cgacggcgcg
    21781 gcacgcccta gaggatcgca gcgggcgtgc ggctactgcg tgcagtacgg caggccgagc
    21841 tcggcgaagc gctcggcggc gttctcggcg aggagctgga tctgcggcac gtgggtcgcg
    21901 agcgccgcgt ccgggttcgg cgcgctgcgc gcgtcgcgca gggcgcggtc cagcgcgcga
    21961 ttgagctgac tgtggaggcg gatgacctcc ttcacgtcgg cccggacgtc cgcgggcggc
    22021 ttcgggagct gctcgatctt gcgctgctcc tggcggaaga agatgcggaa ggtgaggatg
    22081 ttgttcccct cgagcgactt catcgcctcg gtctggcgca cgcgcttctc gcagatcttg
    22141 tcgatcttgg ccatgtacgg gtgctcgctg cggaagtact cgagcagcgg cttgtcaggc
    22201 agcgccgcgc ctgcggccag cgccgcgcca atccacagga cggggtagac cacgatcagg
    22261 ggcgagtcgg gcgccgcctt gaacgggcgg tcgttgtcgc ggacgcaggt gccggcggcg
    22321 atgtcgccga tccgtgcgcg gcgcgggccc gtcaggagca gcgagatgcc gcccacgatc
    22381 gggaacacgg cgaccgcgag cgcgtcgtcg acgaagcgga agatcgtgcg cgccgcgatc
    22441 ctgttggccg gcacgccgtt gccgtcgagg ccgaccacgc gcaggcgcat cgccttcttg
    22501 ccgagcgtct ggccgaacgc ggtctcgagg accatgtagt aggacagctt cagcgccagg
    22561 tacgtcatca gcatccccac ggcggcgccg gcgaagagga agaaacccac cagcgcgccg
    22621 agggcgagca cgatctcgtc gatcacgtgg gcgaacaggc ggcggcggtc gaggcggcgg
    22681 cgatcctcga ggcgcgggtt cacgcgcacg tcctcgccgg tcacggacgc cgccgccacc
    22741 gcggcggcgg tgggcatggc gtcgagcgac tccggcacat cgcgcctcgg ctgctcgggc
    22801 gggaactcgg agccccagcc cggcacctcg atctgttcgg ggttgtaatc cggcactacc
    22861 ggggctgtat cgagcgccgg cgtgccgagc tttagtgcga gtgctgtccc cggaagcggg
    22921 agttcttgat ggaaacgcgc gcgtagaccg gcttgctggt gttcccggcg ttgtcctgag
    22981 ccgtcagccg gaacgtgtag cgcccgctct tgcgcgcctt gtagttccac ttccggcgct
    23041 tggtgccggt ccggatcgtc ttccacttct tgccgctctt gacctggatc ttgagcgtct
    23101 tcaagccgct tccgcccgta tcgaagaccg agtaggtgag gtggatgcgg tggtggctcg
    23161 ggtggcgtcg ggccttgagc ctcacggtcg gcgccgtgcg atcgacgaaa ctcgggccgg
    23221 caccgggctg gccggacccg cccccgccct cggccggcgg cacgggctga gaccccggtt
    23281 gcgcgggatt gtcgaaggtc tcgccgtact cctgccccgc ctccggcccg cggccgttgg
    23341 cggtctggtc acccggaagc atgaccatcc gccactcgga gacgatctcg ttgagcggga
    23401 ccgtctgacg cgcgttgtcg gagtccacga ggaacagcag gtgcagcccg tgcgttccga
    23461 tgctgccctg gaagaacttc tcggccgagt tgggctcgcc gtcaccgggc ttccagcagc
    23521 ggcgctgctc gccggttgag gtcgtgcgcg gctcgtcgcc ggagcgcagc ttgaccttgg
    23581 gaccggggtc actgcccgtg ccgtcgtcaa agcacgagtc gtcgcggtag tacggcgtgg
    23641 cgaccaccga ctgcgccgtg ccgccgggcg agaggtcacg cacgtcggtc gtgatccggt
    23701 cgacgatcgt gccgtacggc ccggcgatct cgttccactc gagcgccacg ttggccttgc
    23761 tgaacgtcgg atcgaacgcg tcgatcgact ggtggtatgg gcgcccgcag agcgactcga
    23821 cgggattcac gccctgcggc ccgtccggga actgacccgc ttccggcgga cagtcgatcg
    23881 cgccattgca cacatccagc tcctgataga tcgtgcggta gccctcgtcg agctgactcg
    23941 tctcgttctg gtcccagttg ctgttgcacg gatcgtcgaa gttcccgaac acctcgtcgt
    24001 ttctgccgtc gacatcgacc ccgtcgaggc gcgacgagtt gtagaagcgc ttcatccggt
    24061 tcgcgttgaa gtcccactgc gcgtagatgc cgtcgagcgg cgggatcacg tgcacgcgca
    24121 gccaggtctt ctggcgcatc tcgtggcggt agaagctctc gcgccggacg acgttggtgc
    24181 cggagtcggc gccccaggtc tcgcggatcg cgcgcacggg accgaccttc tcgccgagca
    24241 gcgtgctcga gccgccccag ttcgtgtcct cctcctcgaa cccacagcag ggcgtctccg
    24301 agccggggtc ctgggcgaac gcgcgcgcct tccagcgatc cacgacatcc ggcgcgtagg
    24361 tcttcccctt gtccggcgag atgttgatct cggtcatcag ccaacggccg tcgtagcgga
    24421 agcggtagcg gtcggtcttg atcgtcgcgt agtcccgcgg ccggcgcttg tcgatcgcgg
    24481 gcttgccctt cttggggtcg atgacgacgt tgccgtcctt gtcgcagtac acgccctcgc
    24541 gcgcgttgcc gtaatcgtcg tagctcgaga cggagcgttc gaaggtgtcc gagaccttgt
    24601 cgcgcttgta ctcgacgtaa ccgttctccg cggtgtacgc gggcttcgga ccgctcggcg
    24661 aggcctgcat gacgtagacg tacttcgtgc cctcgcggcc ggtggggtcg ttcacgacga
    24721 cctcgtaggg agcgccgtcg atgccggccg gcagcggtgt gttcgcgccg atccggtcgc
    24781 ccgcgtccgt gtacatgaac gcgagctcgt cgttgtcgtc gaggcccggg acgggatcct
    24841 tcgcgggctc gtccgctggg cgcgcgaggc acgggttctt cgggtcctcc tcgaaccagc
    24901 ggaagccctc gcgctcgtac gcgtaggtcg tgtgctggtc ctcgcccgag tagatggcga
    24961 agcccgacgc ggagttgttc aggtagcggg tgaagacctc gtccacctgg aacgggatct
    25021 gcttccacgt cttcttggcg gcgtcgtagc ggtagccgag cagggagtcc accgcgggac
    25081 cggcacccag gaagtcacgc gtgtccgcgt ccggctgcgg gttgtagttg tgcgggcacg
    25141 gcggcgtgtt cggcggcttc gggaactcct tgggaagctg atttgcctga cattggaagt
    25201 ccaccaaagg caggcggaac gtctggttcg agcgcgccga ccacagcggg aggtccgacc
    25261 cgcgcaggat cacgggctcg gtgtcgcgct tggctggggg cggctggttg gcgtccacgc
    25321 cgctgggcgc gggctgcgtg gacggcgcgg gcaactgcgc ggatgccgac gcgggtagcg
    25381 cgagtacgac gagtatgagc gcgagtcgct tcagaagatc cgtcccccgg ggcctgaagg
    25441 tagtaccaca ggagggggcc gaccactcgc gaactttcgc ggcgcgcctc agtctccaca
    25501 tgcggggcgg cgcccctcga cggcaccgcg cggcggcggc gggagtcgcc ggcctcgccg
    25561 ccggccgttg atgggctcga gttccccgat catgcgcttc gcggtttcga cgtcgtcgac
    25621 ccagagcgcg accgtcgtgc gtccggcgaa aacctcgacc ttttcaaacg ctccgctccc
    25681 gtccgagaag acggcgaccg gcgctccgcg aagcgagagc atgcgcggcc gtgcgcggcc
    25741 ccggtagcgc gacaggttct ccttgcagat cgggaagttc tggatcgacg ccggcggcgc
    25801 gcaggacgtc tctgcccccg gctcgggctc acaggtcccg tacacgaagc tgatcggccc
    25861 tcgggggctg tcctcgacat cgtcaccacc gccgttcgtc agcggaagct cctcgaacga
    25921 gggccccgcg tagtagaggg cgaacggctt gaactggcgc gcggcgctga cgtcggcggc
    25981 accctgcgct ggcgcatcgt tggagccaca tccgctcgcc aggccggcga gcagggcgag
    26041 accgacggca gcgccggcag cgctacttcc tgccgacacg gatgatcgcc acgtgcccgc
    26101 ttccgtggac ctggtacttg ccgccgcact ggttgaccgg cgcggtgtgg ccccagtact
    26161 cggtacgcgc gaagtgcttg tgctcaccgg tcttcagcgc cagatacaga cgctgccgcg
    26221 catagtcgta gccgctgccc gttcctccca cgggcaggtc catgtggcgc ggtacgtgat
    26281 cgccttggca cttcccgtga tcgtgttcgt ccatgtgcgg cgtcgccgga acgacgctgt
    26341 gcttgccgta cttgtccttg ggcgggtcga ggtgctccca gaagcgtgcg tggtagtcga
    26401 gcgtgtcgct cttgtgcttc cccatggtcc caatcatctt gtagcacccg cccgagtggg
    26461 ccatcttctg cacacccttg gtgttgtagt cccagccggt gtgcttctcg atgtgcccgt
    26521 ggtggacctt ggtgcccgcg tcatagctcc cgatgaagcc ccagtgggct gcggtgccgt
    26581 ggaagatcgc gccgatcgga tcgatccgct gacgtgagct cttgcagtcg gacccgtctg
    26641 ggtacgacca cgatcctccg cgccagaacg catgcgaatc cgaaggcgag accgccgccg
    26701 acagcgcaag cgcagttacc accagggtga cccaggcgac ggcggaccgc ttcggtgcgc
    26761 ggctgatcac tggcatggga gtcgtccttt caaagcgcca ggcaccggcg gcgggagctt
    26821 tccgccgatg tcgaggcggt tgattggtgc tagcgcgccc ggcgcggccc cggcgggctc
    26881 ctgtcgcagc gcgctcacgg ctcgcgacgt ctggtcggcg gtttcgccga agacgacgag
    26941 ggttgcatcc tcggtgtaga cctcagcgcg cagggctgtg tcgaacagcg cgccgggcac
    27001 tccgcgcagc gtggtcaaca cgtagtcgag ctggaagtcc tcgatcccgc tctgcgcgta
    27061 cgaggacagg tttcgctcac acgccggcca cgactgcacc acgacgggcg gctggcaggc
    27121 gccgagcacc gggtccggcg tgcaggtgcc gtagatgaac tccacgaagt tcgggcgcgg
    27181 cagctcgtgc ggggacggga cctcgcagat ccggttgatc gcggtgatgg gcatgccatc
    27241 gaacgacgag ccgagcgagt aggtggggaa gttggctggc tcgtcctcgg aggtgcacgg
    27301 caacaggccc agagccgccg ccacctcgtc ggcggccgcg gaatcgctgc ggctcgccgc
    27361 cgcgccgccc gcgggatcgt cggactccgt cccggggtcg ccgtggtcgg gcgcctcgcc
    27421 cgccacccgc tcgacgtcga tgatccagct ctccgacgtc cgggtgtgcc ctagcgcgtc
    27481 gcggcccgtc accgagatcg tgtgctcacc gtcgctgaag ttctcgggaa cgaactcgaa
    27541 tcgatgggtg aatgtgcaac cgccgccggg acaggcccca gacgccgacg cgacggaaac
    27601 cccgtcgacg aggaggtcgg cgccggtgcc gcccgacccc gcgtcgctca ctgccacgtc
    27661 gaggaacggg cggtccccgt cggcgttcgg ccggtagtcc tccgcgtcgc ggagttcgcc
    27721 gctcaagccg atcgacgagg gcggagagtt gtcgacgccg acgacggcct ctggggtcca
    27781 gccgacattc gcgccgcccc aggcgcctga gaccaggtcg cgcttctgag cgcggacgtt
    27841 gtagtcgccg tcagagaagc tcgtcgtgga ccacgggcat gagtacttgc cgtctgcggc
    27901 acctgcggtc ggcaccgggg cgcacaggtc ctgccaggag cctccgacgc gctgcacctg
    27961 cgcctgccag tccatcgggc cggagtgcgg gtcggtcatg ggaccactca ccgtcacgtc
    28021 gcccgacacg aagcgcggaa gcgagtcgag cccgccgtcg ggcggactgt ggtcccagtt
    28081 cgcgctcggg ttggtcacgt gaactgtctt cgcgccgttg ttcaccttgg cacgcagctg
    28141 gacgttgtgt gtaccgctgg ggaccgagtt cgtgtcgaag cagaactggc cggtcggggt
    28201 ggtctgggtg cctctcacag ctccatccac gagcagatcg atctgctgga accctcgagc
    28261 cggctcgtac caccgctccg catgcgtgcc tacgacgtag atgtccgcgt cgctgaccac
    28321 gtaatcggcg cagcgcgttc cgtgcatcca gtcggacttg gtgagcgctc ccgtccaggt
    28381 gacgcgtgcg tcgcgaggct ggtaggcgac gtcgtggtgg acgaactcgc ccccgttggg
    28441 ctcgttcata cgcggtctcc agctccggcg cagaggtgac aggcgggccg cggtcgcgcc
    28501 gcctggctcg atcgcgaact cgttgacggc gagcggtccc ccgtcgcgcg gctcttgaac
    28561 ggacaggaac cagcgcatcc cccataccgc gtcgatgccg ccttgctcgc ccttcgacga
    28621 gcacgcgttg ccgccggtgg cctcgtcggc ctgaacctcg tcgagcggca ccggctgaac
    28681 ggcctcgaca cgagaggcga agcggcactg cgcgaggacc ccgttcatct ccgggacggt
    28741 cgcgatgtcc caccagagct ctccggcggt cgagtccaac ctctggacgt gccaggacgc
    28801 acccttctcc gcgcctgtct gcgaatcgag tacaaccaca gcgccgccac cgagaccgcc
    28861 catgtaggtg atccacaact cgccccaggc tgcgtccact cccgtgggca tgagctgcca
    28921 cacgtactcg ccgaagcctg ccggcaccgt gaacctgcgc ctcagtgagc cgctggcggc
    28981 atcgaagacg aggatgtccg cccccgagcc gtccatgacg aagacctcgc cgcggtaata
    29041 ggtgattccg cggggctcgc cgagagccgc gtcggagatc gtcagcttgt gcgcgagagt
    29101 ggcgccatcg tagacctcga cacgggcgtc ggggtagctc gtcgagacga agaggtcgcc
    29161 accgcctgta gcggctgaga cgaacgcggg gcagtggttg aactggcccg gatcgatcag
    29221 gaccccttca caggcctcgt cccacgcgaa ggtcccgaag ccttggtcgt agaccggttg
    29281 gacccaccag tcgggctgct ccacggtcgg gaccggttct tcagcgcacc cgcctatcgc
    29341 cgccatgacc agcaggagga gcccggtcct ggccagccgg ttcagccgat ttgcacgcga
    29401 gccccccatt gctcgtgggg catacctcgt ggcgcaaaaa cgctaacgag aatttttagc
    29461 gcttggcggc cgcttacttg atgacggcga tcgtgtcgcc cgtggcgacc gcgccgccct
    29521 ccgacacgtt gagctcctcg acctggccgg ccgcgggtgc ggtgatctcg ttctccatct
    29581 tcatcgcctc gatcacgcag atgagcgcgc cctcggtgac ctcggcgccc ttctcgaccg
    29641 ccaccttgag cacggtgccc tggatcggcg agaccagttc gcccggcgcg ccggcggcgg
    29701 cgcccccgcc gcgctcgcgg cgcttggggg ccttgcgcgc gcccgtcgcc gctgcggccg
    29761 gggcgccccc gttcccgccg ccgaggcccg cgccgatcac cttcacgtcg aagcgccgcc
    29821 cggagacctc gaccgcgtat tcgcgctcga ccttctcctc ctcgtcctcg ccggcggccg
    29881 gaggcgctgc cggggggtcg ggagcggcgt tctccttcag ccacttcttg tcctcgacga
    29941 ggtcgcggca ggtctcgccc gccgcccact gcggcgtgtc gagcagcgcg acgtggaagt
    30001 tgatgagcga cttgagcccg gccagctcgt actcgcgcag cgcgcggatc atccggcgcg
    30061 tggcggcctc gcggtccacg tcccagacga tcagcttcgc gatcatcggg tcgtagagcg
    30121 gggtgatctc ggagccggcc tcgacgcccg agtccacgcg cacgcccggg cccgcgggct
    30181 ctcggtaggc ggcgatccgg ccgggtgccg gagcgaaggt cttcgacgcg tcctcggcgt
    30241 tcacgcggca ctcgatggcg tggccgtcca gccgcacgtc ctcctggccg agcgagagct
    30301 cctcgcccgc cgcgatccgg acctgctcct tcacgatgtc cacgccggtg accatctcgg
    30361 tcacgcagtg ctccacctgc acgcgcgtgt tcatctcgag gaagaagtac tcgccgtcct
    30421 ggagcaggcc ctcgaccgtg ccggcggagc ggtagccgac cgcgcgggcc gcctcggtgc
    30481 cgatcgagcc gatgcgctcg cgcagcgcct cgtccacggc gggcgcgggg ctctcctcga
    30541 tcagcttctg gtggcggcgc tggaccgagc agtcgcgctc gccgaggtgg atgacgttgc
    30601 cgtgcgagtc ggcgaggatc tggacctcga cgtggcgcgg gtcgggcagg tagcgctcga
    30661 ggtacacggt cgcgtccccg aagaacttct cgccctcgcg cgcggcgccg tcgaaggcct
    30721 tctcgagctc gctcgcctcg agcgcgacgc ggaagccctt gcccccgccg ccgcccgccg
    30781 ccttgacggc gatcgggtag ccgatctcct cggcgatctt cgcggcgtcg tcgaccgtgt
    30841 cgaccgggtc ggtcgtgccc ggcacgatcg gaacgccggc gttcttcatg atctcgcgcg
    30901 cgcgggtctt cgaccccatc gcctcgatgg ccgaggcggg cgggccgatg aacacgatcc
    30961 ccgcctcctc gcacgccttc gcgaacggag cgttctcggc gaggaagccg tagcccggat
    31021 ggacggcctc ggcgccggac ttctgcgcgg cgtcgatgat cttctcgatc accaggtagc
    31081 tctcggtggc cggtccgggg cccagcaggt aggactcgcc ggcgcgcctg acgtgcagcg
    31141 cgtcgcggtc ggactcggag tagacgccga ccgtggcgac gcccatctcc tcgagcgcgc
    31201 gcatcacgcg aatggcaatc tcaccgcggt tcgcgaccag gaccttggag aacatcggcg
    31261 cggaagccta ccgggggcac gcaactcccg cgcgccgcgc ccgctgccat ggcggaccgc
    31321 ccgtgccggc cggggtgtct ccaccttcgg cggggtcgcc agcatgtctt actatcctaa
    31381 ctgttagaga cgttaacgag acgggtgccg cttggcgcag ggggctggta gcggtttcct
    31441 aactatcata actgttactt tagttaggtg ctgtccggcc ggccccttca aggcacatcg
    31501 cacgatctcg atctcttcgt cgatcgcgag ggcgtgatgg accgaattca cgattcagcc
    31561 gcacgccggc tgaacaccct ggtggtgggc gaacgtcgct ccgggaagac ctcgctcctg
    31621 cgcgtgctgc aggggcggct ggcttcccgt ttccacgtgg tgatgctgga tgcgggcctg
    31681 tcgcacgacg cagcttcgtt tctgtcgctg ttcctcttct cgctcgagcg cgaagcagcg
    31741 ctcgggcggg cgggcgctgt gtccaacccg cggctcgacc tcgactactc gttcgccctg
    31801 cccgccaccg agacagagcg tgtgctcacg ctgctcgatg ccgtccgcca cgcgatcgaa
    31861 cgtcgcgagc gtggcgttgt cgcgatcgtc gatgacttgc gtacggccga gctggcccat
    31921 cgcctgttcg gccggatgcg cgacgagctc tggatgctcg ggtgcacctg gatcgtggct
    31981 gctgacactc aggacagaag tgcgttgctg cgtcctcccg ccgacgcctt cttcgagacc
    32041 gtcatcgaac tggggccact ggaccatcgc gacgcggccg agctcgtccg ccggcgactc
    32101 gccgacgagg acgccgatcc cgacttggtg gagcgactcg cacgcgccgg ggagggaaac
    32161 ccggggcgca cgatcgagct cgctcgcgag gtcgtggtcg acggcatacc gctcggtgag
    32221 gcgacttcgc gccggaccga cgtccggttc cgagcgggca agctcggcga ctcggcacgg
    32281 cgactcgtcg aggacctact cacctacggg ccggcaagcg cctctgacga gagcctgctg
    32341 cgccggctga attggacgcg ggcgcgcgcc acgcaggtct tcaagcagct cgaggcagag
    32401 ggcatagtcg gcgctgtccc agcgcggagc ggtggggggc gaaaggtcta cgggctgaac
    32461 ctgaacggtg aggcatgacc gcccgcgatc tcatagccac gctccgcgag cgacacgcac
    32521 tggacccccg gccgctaccc acggatctat cggcctacca cgtgccattc gacactctga
    32581 acggcgacga gcggacggag tccgcgctcg cgcgcgccgc ggtcgccatg gagcgcatcg
    32641 ccctgatcgg cgacggtggt gtcggtaagt cgagcgtgat ctcacacgtc ttccggctcg
    32701 gttccggctt cgcgcctgtg gtcgtgcccg tgttcgacga gcgcgccgcg gtcgtgtccg
    32761 acccgggcgc attcgcagct cacgcggtcc aggtcctggc gcggtacgcc gaccaagtcg
    32821 agttgacagc tccccgcgag cgcgatcgct atctgcgggc catcgccgag cgcacccacc
    32881 tcccgagcac ggagaggggt ctgcgggcgg gcatcaagct cgcgccgtgg atcgcgcagt
    32941 tcgatgtagg agccgacctg aagcgggtca ccccgcctgc cgagatccag ccgagcgcga
    33001 gtcagtacgt ggccgctctc gacgacatga tcgcggtcgt caaacggacg gagctgacgc
    33061 cggtcctcat catcgacgac tcggatcgct ggcttcggct cgacgacggg gaaccgcgca
    33121 acgacgtcgt agagccgttc ctcgcgcacg tgcttcggat gctggcggat cgcgcgatcg
    33181 ggttcgttgt cgcgatccac aggacgtatg tcgatctcgc gggctatgcg tccgcggtcg
    33241 agcagggcac gctcgagacg ttcatcgacg taccagccct ggggtccggc gtcgctctcg
    33301 aagcgctcct ccaacatcgc gtgcagctcc atgcgccgga ggcgtcgctt gcggacgtgt
    33361 tcaccggtgc ggcgctgtcc gagttgtgga ccgtctaccg gggaggggcg gacggtcaga
    33421 tccgcaagac actccaggtc gctcacggtg cgctggctga cgccgacgcg tcgacgcttg
    33481 aggggccgat cgatcggcca ctcgtacagg caagcgccgt ggcggctctg cgctagggct
    33541 agcgctcagg cgcggtgacg tgccgcggcg gggcgggcac ctggatgcgc gccgccgcct
    33601 ggcgccgctc acgcagccac gcccgcagcg gcgggccgca gacgatcgcg gcgagcaccg
    33661 cggggatgcc cgcgatcacc ggggtcggga ttcccgcgcc cgccttggcc gccagcgcca
    33721 ggccggctcc gatcagcacc acgccgagca ccgtgcgcag cgtcgccacc ggcacgcgca
    33781 ccgaccagct cgtgccgatc cagacgcccg ggacggagcc gagcaggatc gtgcccgcca
    33841 gcgcgtaatc gacgttcccg gcgaccacgt gcgcgatggc agcggcccac agcaggagcg
    33901 ccgcgtggaa cacgtcggtg ccgaccacct gccgcggagc caggtggaac aggaggatca
    33961 ggccgaccgc gatcagcgat ccgctgcccg ccgacgtcac gccgagcacg aacccgacga
    34021 acacgccgag cacgacggcc gagatcttgt ggcggcgctc gagcacgaac gactcgcgct
    34081 cacgctcgac cagcttcggc aggaacagcg cccgcgccag caccgccacg cccgtgaaca
    34141 gcagcgcgcc cgcgagcacc gcgagcatcg ccccgtcgaa gttgcgtcca ctggctcgct
    34201 cgatcgccgc cagcgcatac acgccggaga tggccgcggg cacggagccg agcgccatcc
    34261 aggtcgagag cgacgagttg acggtctttt ggcggaagtg ccgccagccg ccgacggtct
    34321 tcgtcaccgc gccgtaggcg aggtcggtcc ccacggccgt gaccggcttg aagccgaaca
    34381 cgaggatcag gatcggcgtc atgagcgacc cgccgcccat gcccgtcatt ccgacgagga
    34441 tcccgacgcc cagcccgaac aggatgatca ggggatccat aggaactctc cctacgccgg
    34501 agtcggctcc ccggcgtacg ccgcgatgag cacgtcggcc acgtccggcc gcgtgaactc
    34561 ctcgggcggg cgctcgccgc gcccgagcat ctcgcgcacc ttcgtgcccg acaggaacac
    34621 ccggtcctcg gggcccgacg gcgaggtctt cgccgtcgcc atcccgctgg tgagcttgca
    34681 ccagaacgag tgctcgaact tcagcggctc gatgccaagc tcgccgggct cgaactcgtc
    34741 gaagatgtgc tgagcgtcgt aggtgccgta gtagtcgccg acgcccgcgt ggtcgcggcc
    34801 gacgatgaag tgcgtgcagc cgtagttctt gcgcacgagc gcgtggaaga tcgcctcgcg
    34861 cggaccggcg tagcgcatcg ccgctgggtt cacgccgagc agcacccgat cgctcgggta
    34921 gtagccctcg atcagcacct cgtagcaccg catccgcgtg tcggcgggga tgtcgtcgtc
    34981 cttggtctcg ccgacgatcg ggtggatgaa cagcccgtcc acgatctcga gcgcgcactt
    35041 ggtgatgtac tcgtgcgcac ggtggatcgg gttgcgggtc tggaagccga cgacggtgtt
    35101 ccagccgcgc tcggcgaagg tccggcgggt ctccttgggc gagagcacgt acggcgcgac
    35161 cgcgtcgatg tggccgggca gcgccttggc ctcgatcggg cccgcgatcg tgcgcgcacc
    35221 ctcgcggtgc agcgcggcga cgcccgggtg cgactcgtcg gtggtcctgt agacctgctg
    35281 cgcctcgcgg gcgaggtcgc gctcgtagac ctcctcgacc gcgagcgtgc cgagctcggt
    35341 gccgtcgctg cccacgagcg tgagcgtctc gcccacctcg ccctggtcgg tcgcgagcgt
    35401 gaccgggatc ggccacggcg ctccgtcggg caggcgcatc gtctccgtga cggtctccca
    35461 tgcagcggat ccctggaagc cggtcagcgg cgcgaagccg ccgttggcga gcagctcgaa
    35521 gtcggcggtc tggcgggcgg tgagcttgag gctgctcacg cgggcacccc gaggcgctcc
    35581 tcgacgaacg cgagcacctt gccggccgac tcctcgggcg tctcgttctc ggtgtcgagc
    35641 acgatctccg gtgccgacgg ctcctcgtac ggatccgaga ccccggtgaa ctccttgatc
    35701 tcgcccttga acgccttctc gtagaggccc ttcacgtcgc gccgcgcgca ctcgtcgacc
    35761 gacgccttca cgtagatctc cacgaagcgc tcgcccatcg tgccgcgcgc ctcgtcgcgg
    35821 atctcgcggt acggggagat cgcggccgtg atcgccacca cgccgttgcg cgagagcagg
    35881 tcggcgacga acgcgatccg gcgcacgttc gtgtcgcggt cctccttcga gaacccgagc
    35941 cccttcgaga ggttcgtgcg caccacgtcg ccgtcgagca cctcgaccct ctgaccgcgc
    36001 tcggcgagct ggtgctcgac gatctgggcg atcgtcgact tacccgcccc cgagagaccg
    36061 gtgaaccaca gcgtcaatcc ttcgctcatc tgatctgacc tccgtagggg gttagccgtg
    36121 cagcccgcac tcggacttgg cactgccggc ccagcggccc tcgcggccgt ggccgggctg
    36181 ggtgcagggc acgcagccga tcgagtcgta gccctggtcg tgcagcttgt tgtagggaac
    36241 gtcgttctcg gcgatgtaac gccagacgtc gcgctcgctc cagtcggcga gcggagcgac
    36301 cttccagagc tggttgcgct cgtcccagtg gaccttgccg gtgctcgcgc gctcgggcga
    36361 gtgctcgcgg cgcaggcccg tgacccacgc ctcggcgtcg ccgagcgcac tgcgcagtgc
    36421 cgccaccttg aagtcgccgc agcagcgctc gccgccggtc cagggaagcc cgtcgggcgc
    36481 cgaggcgtcg tagacctcga tctcgatccc gtagcgctcc tcgatcgcgc gccgggtggc
    36541 gtgcgtctcc tcgaagagga cgccggtgtc gatcgtgacg atccgcgtcg aaggctcgat
    36601 cttcaggagc atgtccgtga tgaccgagga ctccttctgg aaggaggagg cgaggaccag
    36661 ccgcgggtgg tactgctcga ccgcgtagcg cagcacgccc tccgcggacg cgtgctcgag
    36721 gtcgggtgca gtggcggtcg tcaaaccgcg cactcgccct cgccgcggat cacctggtac
    36781 ggctcgtggc ggctccagtc gatgaagtgc tggatgttct ccaggttgaa ctccaccggc
    36841 agcgagagct ccttcgcctc cttctcgaac ggctcggtcc cgacccggtc gacgaacgcg
    36901 ttgaactcct cgccctcctg gcgctcgcgc tcgtagaggc ggatccagcg ctcgacggcg
    36961 tcgggcaccc gcttggccgg aaggcgcacc ttcacgcgct ggccgtacgc gagctcgccg
    37021 ccctcgtagc ggccgccgac gtgggcgatg taggcgggca cctgcttgcc gcccatcttc
    37081 atcgacgccc cgtagaagcc gatcgtgccg aggtggtgct ggccgcagcc gttcgggcag
    37141 ccggacatct tgatgtggat ccgcttggtg agcggatcct cgatgcccat gccctcgacc
    37201 cggtcctgga tcgcacggtt gaggcccatc gagctggtga tgcccatctt gcagctgtcg
    37261 gtgccggggc agctcaggac gtcggtgacc tggtcggcgc cggcgtcgcc gaggccgagc
    37321 tcgaccagcc ggcggtacac gtcgtaggtg gcctcctcgc gcacccagcg gagcacgagg
    37381 ttctggtgca ccgtcgtgcg ggcgtagccg ccgcagtact cgcgcatgat cgcggcgagg
    37441 ccgcggaaca tctccggcgt gaggtcgccg cgcgtgacct tcacctcgac gctgacgaag
    37501 ccctcctgac gctgcgggcg gacgttcgag gcgcggaagg cgtcgaactc ggagcggtcg
    37561 ccgttcggcg aggccacgac cgcgggcggc agcggcgcgt tcgcctcctc gtcgtgctgg
    37621 aagagcagaa gcgtcgggtc gaagttccgc tccctgaccc agtcgccctt gagctcctcg
    37681 tcgacctgct cgcggaacgc gtcgatcccg atcttgtcga ccaggaactt gagccgggcg
    37741 cgctggcggt tcgcgcgcag ccagtcctgg cggttgaaga tccgcatcac ggcctcggcg
    37801 accttcaggt agtcgccgtt ctcgacctcg acgaagtcgt acatcgtgct gccgacgcgg
    37861 gccatgatcg aggtgccgcc gcccgtgttg atctcgacgc ccttgacctc gcggccgtcg
    37921 atctcgcgca cgcgcgggat gaagccgagg tcgtggatct tcgtgatcgc gcggtcgtca
    37981 tcgttcgcgg tgaacgcgac cttgaacttg cgcggcatga gctggcagac ctcgttgcgg
    38041 acgaagtagc ggacgaacgc gccggcgtag ggcgtgatgt cgtagagctc gtcggtgatg
    38101 ccggcccacg gatcgccggt cacgttgcgg acggtgttgc cgcaggcctc gcggctcgag
    38161 agcccggcct cggagagcag gcggatcgcc ttggcggcgt cgggcagcgg gacgtggtgg
    38221 tactggatgt tctgccgcgt ggtgatgtgg cccttgacga gcggcgtgaa gcgctcggcg
    38281 acctcggcga aggcctccat ctggtccggc gagacgccgc cgaacggaag cttcacgcga
    38341 atcatctgga cgttcgcctg gcgctgcccg tacacgccct gcttcaggcg gaaaccgatg
    38401 aactggtcct cctgcacctc accgcgcagg aagcggccgg cctgggtgtc gaagtcgtcg
    38461 aactccttct cgatggtcgg gatcacgtgt cccgggacat cgacgacctc ggggtttttc
    38521 agggagccgg agcggccccg cggctctgtc gcagtcggtt ccatcgcgcg aatgtaccaa
    38581 aacccgccgg ggtttaggta ttttcgcgcg aggcgggccg ggtgcgccgg ccgcggtcta
    38641 gcgcgggggc aggttctgag gcggcgctac ggccgcgccc tcgggctcga gcggtaggcc
    38701 cgcctcgtcc cacgcgacca gaccgccgtc gatgctgtga gcgtcgaacc ctgagttgcg
    38761 cagcgcctcc gctgccatct ccgagcggtt gccgccgcgg cagtagacga gcacggggtc
    38821 gtccgcgctc agcgcctcag cggactcctt gtcgaggcgg tcgagcggaa tgtgcgctgc
    38881 acccgagatg tggctctcgg cgtgctcgcc cgctgtccgc acgtccacca cctgcgcgcc
    38941 ggcatcgatc agctcgcgtg cccgcgcggg gtccagttga agggtcgttt cctcggtcat
    39001 ggcacgccag ggtatagctc agcggtccca cgggttggcg tcgccccacg ggctcggagc
    39061 gtcggccgcg aggcccaccc cctcgaacag cgcggcgcgc agccatgggt cggcgggctc
    39121 gtcggtcggg gcgggcgccg gcgcggtgtc gcgcaggaac tgctcgatcg cggcggcgat
    39181 ggccgcagcc tcctcgggtc ccgccacgga ctgccgaacc tcgatgcgcg gccgcggcgg
    39241 cgcgtgcttt ccgttccgtc ccatcgccgg cccggagggt acaggcgccg ctagaccggg
    39301 tcggtagtcc cctcgttcag gagagtgagg tactcgtcgt acacgtacaa cttcccgcga
    39361 cgccgcccgg tcgcctcgcg aaggatccca atatcttcga gcctggcgat cgcggaatag
    39421 accggaggcc ccgtcaagcc gagcacaccg gccgcagtcg tagctcggat cacgacgtaa
    39481 cggacagcgt gctcatggat gcgtagggcc gttgcggcac cgcgtccgag ggtctgaatc
    39541 ctgcgccgat cgcgctcgac catctcgacg attcgctgtg tagcgtcggt agtcgaagca
    39601 gcgaccgtaa cgacgccctc tagcgaagtg aacgattagt agtcggcgac cttaacaagc
    39661 gaaaaagctg gatcgctttc ttatatagga cgaaggaagc gatagctgtc ttaatgacct
    39721 tccctcgagg acggggcgaa ccggggcggg gccgacgatg gcaggcccgc gttatcgtcg
    39781 gcgggcgtgc cagagatacc ggtgtacgac cacgcgacgg tcctggcggc gatctccgcc
    39841 gccgacgcgg tcgagcgcgt gcgcgacggc ttcgtccagt tcgcgcacgg cgactgggag
    39901 atgccgccga aggtctacgt cgacgcgccg ccgcgcggcg acttccgagc catgccggcg
    39961 cgcggcggcg ggcacgcgat cctcaagtgg gtcacgtcgt tcaccggcaa cccggcgcac
    40021 gggctgcccg tggtgcaggg agcgatcctc gtgtcgagcg ccgagaccgg gacgccgctg
    40081 gccctcgtgg acgtgggggc cgtgacgtcg ctgcgcaccg gcgcggtggc ggcggtcgcg
    40141 gctcaggagc tcgcgcgcga ggacgcccgc acggtgggga tcatcggctg cggcgtgaac
    40201 gggagctggg ccgggcgctg cctcgcggcc gccgagtacg ggcccggcgt ctgcttcgac
    40261 ccggaccccg accgcgcggg agcccttgcg ggtgagctcg gctgggaggc cggcagccgc
    40321 gaggacgcgc tcgggtgcga cgtggtcacc tgcatcacgc ccgggcacga gccggtggtg
    40381 cacgcgaccg acctgcgccc cgggatgcac ctctcgatgc tcggcgccga cgccgcgggc
    40441 aaggccgagg ccgacgtgga cgccgtcacg cgctgcgtgc tcttctgcga cgagtggcgc
    40501 caggccagcc acggcggcga gctgacgcag gcggtcgagc agggactcgt cgcgcgcgac
    40561 gacgtgaccg acctcggcgc cgtgatcacc ggcacggcac ccggacggcc ggacgacgac
    40621 gcgatcacgc tgttcgactc gaccgggctc gcgatccagg acctcgcgct ctgcctcgcg
    40681 ctcgtgcggg acccgccgcc ggccacacag acgatcaggc tttgacggcg ggcgccgggg
    40741 cggcgctcag ccggtcaggc cgtgcttctc catgagcggc ccgatgctct ccgggtccat
    40801 ctcgagcccg tagcgcgcgg ccacctgaag gagcgtcgcg aggtccggct ccgggcggtt
    40861 cggcgggagc agcggcgcga tctcctcgaa gtagcgctcg aagcccgccg ggctgatgat
    40921 ctccaggacg cgcgccggct cgtcgccggg gttccagaac gcgtgccact ggttgcgcgg
    40981 cttgaacacc aactcgccgg gcccggcctc gacggtctcg tccccgagct gggcccccat
    41041 gcgcccctcg agcacgtagg agtactcgtc ctcgttgacg tggcgatgca gcggcgcggc
    41101 gagcgtgcgc ggcgcgatcg ggtgctcgac gagcgagaaa ccgccgccgg ccgtctcgcc
    41161 gtcgatcatg aagcggacgc cgaggccgcc gaggtcgacg cggtcgccgt cgttgggtcc
    41221 cacgatcttc gccatcgggt ccgcctttcg ctgtacagtt gtgttcactg gaatggcgag
    41281 gagagtacag cagccacgtg cctatcgcaa gaacgtgcgc gccgagcacg aggcggccac
    41341 acgccggcgg atcaccgagg cgaccgtggc gctgcatcgc gaggtgggtc ccgctcggac
    41401 caccatctcc gccgtcgccg agcgggcggg cgtccagcga ctgaccgtgt accgccactt
    41461 cccggacgag gccgctctgt tcggggcctg ctcgtcgcac tggctcaccg cgcacccgat
    41521 ccccgatccg gcgccgtggg cgcgcgtcgc cgatccggag gagcgactgc gagcggcact
    41581 gggcgcgcta tacgagtggt atcgcggcga cgaggcaatg atcgccaacg tcgggcgcga
    41641 cgcgcccggg ataccggcgc tcgccgcgat cgtcgccaac ggcctccccg cctacctgga
    41701 ggcggtgcac gagctgctga tgcctggctg gggagcccgt ggccggcgtc gccggatgct
    41761 ctccgccgcg ctcgggcacg cgctcgactt cgagacctgg cgttctctcg agcggcgtca
    41821 gggcctcgag ccggagaacg cgatcgagct catggtccgc gccgtgcgcg cagcgttcta
    41881 gcgcgagcgc cgcagcggct cggcgcgcgc cgccagcaag aacgacaccc cgcgcatccc
    41941 gtcgcgcagc ggcgcgagct tggactcgcc cccgcgcgga tgcagctcga tgtcgagctc
    42001 gcggatgtcc aggccctcct gcgccgcgcg gacgaccatc tcgagcgcga actccatgcc
    42061 gccggagcgc aggcgcagcc gctcgagcgc gtcgcggcgg atcgcgcgca ggccgcacca
    42121 gacgtcgcct atccctgggc ggaacagggc gcgcaccagg ccggtcgtga gcgggttgcc
    42181 gaggtagcgg ttgagcacgg gcatcgcgcc gggcgcgatc gcgcccatcc ggttgccgag
    42241 gacgagatcg gcgccgtcct cgagctcgcg cacgaaccgg gggatctcgc cgaagtcgta
    42301 ggagaggtcg ccgtcgccca tcacgatcag ctccccgcgc gccgccgcca ggccggccag
    42361 gtaggcgcgg ccgtagccgc ggcgcggctc gctcaccacc cgcgcgcccg cggcggcggc
    42421 cagctcggcg ctgccgtcgg tcgagccgtt gtcggccacg atcacctcgc cacccgggcc
    42481 cgccgcgcct cgcgcgacgc gcacgcagtc cgcgatggcc gcggcctcgt ccaggcacgg
    42541 gatgaccacc gacgttcgtg tctgcggcgc ccccaccggc gcggcactct acccgcgcgg
    42601 gcagcgggcg agcacggtcc aggtgcggtt ggccgccacg cgctcgaagc ccggcgggcg
    42661 cagcgggcgc tcctcgagcg agtagatcag cagcatgccg gcggacagct cgagcctgcg
    42721 ggtggcgacg atccgcgcgg gcgggtcgtc gcggcgcgcc cagagcaggg tgggccgcgc
    42781 gcggaacagg cgggtctgga cgggctggca gcgcgggatc tgctcgcgga cgcgcggctc
    42841 ctcggcgatc gcgcgcagat cgtcctggag gcgctgcgcg cgatcgacct tctcgagcgc
    42901 gccgctcatg tggtcaacaa ggaacggcac gtgcacgagc agcgcgactc ccatcgccag
    42961 cccggccacg cgcgccacgc cgcgctcgcg gctcgcctcg ggcagctcgc gccaggcgag
    43021 cgtgccggcg gccgccagca ggcagagcat cgtcgcgggc atgaagaagt agcgcgtgag
    43081 cagtggcagg ccggcgaacc cgatcacgag gaacgacgcc gtgccgagcg cgagcagcgc
    43141 gagcggcacc tgcgcgcgcc gccgccacag ccacgcgacg atcgcgagcc cggccgtggc
    43201 gacgagcgcc gggaccaggt gcaggatccc cttccacgac cgcgcggccc actcgatcgt
    43261 cgccgcgggg ccgccgtcgc cgtagcgctc ggcgtcggcg agccgctcgc tggtggcgcg
    43321 cgcctggtgg aacgaccaca gggcgtcgcc ggtgatcacg agatccgagg cgatccacgc
    43381 gagcggcggc agcgcggcga gcgcgatcag cttcgcgcgt tcgcggcccc gcgcgtcacg
    43441 caggagcagc agcgtgtaga cgcctgttat gagccacgcc tcgggacgga tcagccccgc
    43501 gacgccgagc aggatgagca ccggcacgcc cgcccacggc cggcgcacct cgatcgccgc
    43561 cgccgcgacg atcagggcga ggaacggcac gtcgatcgac gcgatcagcg cctggttcac
    43621 gagcgagggg cgcgtgagga gcagggccgc gaacagaacc ccgaccgcgc gcgagaacag
    43681 ccgcgcgccg agcacgtagg ccgcgactcc gagcgccccg taggacacga ggctcagcgc
    43741 cttgagc
//
LOCUS       DASVPL010000299         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_232187, whole genome shotgun sequence.
ACCESSION   DASVPL010000299 DASVPL010000000
VERSION     DASVPL010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232187"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF517_16995"
     CDS             <1..241
                     /locus_tag="VF517_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054687.1"
                     /translation="LDRARRLHQFRIRRFKARGGYLEDGDGIEDRSQAYQRLMRDLLG
                     VQRDALVRLRNGGEISNDVMHRVVRELDLEESRLA"
     gene            255..1793
                     /locus_tag="VF517_17000"
     CDS             255..1793
                     /locus_tag="VF517_17000"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8054688.1"
                     /translation="MRRPRPLQRLIDGRPDDSGRRIAWRSTLLIAPAMAAANAVGALV
                     VFVLATYVVPSPEFSDRDEAVVWNLVAAAIYVLAACAVGALWGGRILRPVRAWLREER
                     DPDQREQRMILRSPLRLLFVNASLWTLGVAVFTALNLLWSGALAVKVALTVALGGLTT
                     SATTYLLTERISRPVTARALRSGAPERPVLPGVTTRALLAWALGSAVPVLGLLLVAIA
                     SLAGRDVSEREVAITTLGLGGVVLATGLLVTWQAARAVSAPVVAVREALRRVEAGDYE
                     TEVPVFDGSELGLLQAGFNRMEAGLRERERMRDVFGRQVGEEVARSALERGIELGGEE
                     LDVAVLFVDLVGSTTLASERSPTEVVELLNRFFDVVVDVVESRDGMVNKFEGDAALAV
                     FGAPIPRDDAAACALAAARDLARRLEVADIGLEAGIGVSAGRAVAGNIGAERRFEYTV
                     IGDPVNEASRLCELAKSRDGRVLASERAVEAAGESEAAHWRLGEEVELRGRTRPTRLA
                     VPAG"
     gene            complement(1790..>3003)
                     /locus_tag="VF517_17005"
     CDS             complement(1790..>3003)
                     /locus_tag="VF517_17005"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8054689.1"
                     /translation="RVLLLASRFKPAWILGTVANSSAKILENMEIGHNVLHGQWDWMN
                     DPYIHSTTWDWDTASTSEAWKHSHNYIHHTYTNIRGKDKDLGYEIMRIDPHQKWHPVY
                     LLQPIYNILLASLFEWGVALHDMDLEAIRAGEKSHKEALEDLKGIAGKARTQIVKDYV
                     AWPLISALAAGAVSVALEPRRMTRREKLAARARGLLPNRRGRTEIAKELAVRVAAGGA
                     RTFRATVTANVTANIVRNFWSYAIIFCGHFPDQTYTYSQKEVEDETRGGWYVRQLTGA
                     ANIEGSPFFHVASGNLGYQVEHHLFPDMPSTRYAEIAPRVKDICERYELPYNSGPFGQ
                     QLFQVQRTIARLAFPGGKPRPKPGPYRGGDGEASSNGHVNGNRRKKRSQESSGPEQPS
                     DGVRVSIPSQN"
BASE COUNT          356 a         1060 c         1110 g          477 t
ORIGIN      
        1 gctcgaccgc gcgcggcgcc tgcaccagtt ccggatccgc cgcttcaagg cgcgcggcgg
       61 gtatctggag gacggcgacg ggatcgagga ccgatcgcag gcctaccagc gcctgatgcg
      121 cgacctcctg ggcgtccagc gcgacgcgct cgtccggctg cgcaacggcg gcgagatctc
      181 gaacgacgtg atgcaccgcg tggtgcgcga gctcgacctc gaggagtcgc ggctcgcctg
      241 acaggcttat ctgcgtgcgc cgaccccgcc cccttcagcg cctgatcgac ggccggcccg
      301 acgactcggg ccgccgcatc gcctggcgct ccaccctgct gatcgcgccg gcgatggccg
      361 ccgccaacgc ggtcggcgcg ctggtcgtct tcgtcctcgc gacctacgtc gtccccagcc
      421 cggagttctc cgatcgggac gaggccgtcg tgtggaacct cgtcgcggcc gcgatctacg
      481 tgctggcggc ctgcgccgtc ggggccttgt ggggcgggcg gatcctgcgg cccgtgcgcg
      541 cgtggctacg cgaggagcgc gaccccgacc agcgcgagca gcggatgatc ctgcgctcgc
      601 cgctgcggct cctcttcgtg aacgcgtcgc tgtggaccct cggggtggcg gtcttcaccg
      661 cgctgaacct cctctggtcg ggcgccctgg ccgtgaaggt cgcgctcacc gtcgcgctcg
      721 gcgggctgac cacttcggcc acgacctacc tgctcacgga gcggatcagc cggccggtca
      781 ctgcgcgcgc gctgcgctcc ggcgcacccg agcggccggt gctgccgggg gtgaccacgc
      841 gggcgctgct ggcgtgggcg ctcggcagcg cagtgccggt gctcggcctg ctgctggtgg
      901 caatcgcgtc gctcgcgggc cgcgacgtga gcgagcgcga ggtcgcgatc accacgctcg
      961 ggctcggcgg ggtggtcctg gcgaccgggc tgctcgtgac ctggcaggcc gcgcgcgcgg
     1021 tgtcggcccc ggtcgtcgcg gtgcgcgagg ccctgcggcg cgtcgaggcc ggcgactacg
     1081 agaccgaggt gccggtattc gacggcagcg agctcgggct gctgcaggcc gggttcaacc
     1141 ggatggaggc gggcctgcgc gagcgcgagc ggatgcgcga cgtcttcggc cgccaggtcg
     1201 gcgaggaggt ggcccgctcg gcgctcgagc gcggcatcga gctgggcggc gaggagctcg
     1261 acgtcgcggt gctgttcgtg gacctcgtcg gctcgaccac gctcgcttcg gagcgttcgc
     1321 cgaccgaggt cgtggagctg ctcaaccgct tcttcgacgt ggtggtggac gtcgtcgaga
     1381 gccgcgacgg catggtcaac aagttcgagg gcgacgcggc cctcgcggtc ttcggagcgc
     1441 cgatcccgcg cgacgacgca gccgcctgcg cgctcgcggc cgcgcgcgac ctggcgcggc
     1501 ggctcgaagt ggcggacatc ggtctcgagg cgggcatcgg cgtctccgcg gggcgcgccg
     1561 tcgccggcaa catcggggcc gagcgccgct tcgagtacac cgtcatcggc gaccccgtga
     1621 acgaggcgtc gcgcctgtgc gagctggcca agtcgcgcga cggtcgcgtg ctcgcgtcgg
     1681 agcgcgcggt tgaggcggcc ggcgagagcg aggccgcgca ctggcggctc ggcgaggagg
     1741 tcgagctgcg cggccgcacg cggccgaccc gcctcgccgt gccggccggc tagttctgcg
     1801 agggaatcga gacgcgcacg ccgtccgacg gctgctcggg ccccgagctc tcctgcgagc
     1861 gcttcttgcg ccggttgccg ttcacgtgcc cgttcgacga cgcctcgccg tcgccgccgc
     1921 ggtacgggcc cggcttgggc cgcggcttcc cgcccgggaa cgccagccgt gcaatcgtcc
     1981 gctgcacctg gaagagctgc tgcccgaacg gccccgagtt gtacgggagc tcgtagcgct
     2041 cgcagatgtc cttcacgcgc ggcgctatct ccgcgtagcg cgtgctcggc atgtcgggga
     2101 acaggtggtg ctcgacctgg tagccgaggt tgccgctcgc cacgtggaag aacgggctgc
     2161 cctcgatgtt ggccgcgccg gtgagctggc gcacgtacca gccgccgcgg gtctcgtcct
     2221 cgacctcctt ctggctgtac gtgtaggtct ggtcggggaa gtggccgcag aagatgatcg
     2281 cgtaggacca gaagttgcgg acgatgttcg cggtcacgtt cgccgtcacc gtggcgcgga
     2341 acgtgcgcgc gccccccgcc gccacgcgga ccgccagctc cttggcgatc tcggtccggc
     2401 cgcgccggtt cggaagcagc ccgcgcgccc gcgccgcgag cttctcgcgc cgcgtcatgc
     2461 gccgcggctc gagcgccacg ctcacggccc cggccgccag cgcgctgatc aggggccacg
     2521 ccacgtagtc cttgacgatc tgcgtgcgcg ccttgcccgc gatgccctta aggtcctcga
     2581 gcgcctcctt gtgcgacttc tcgccggcgc ggatcgcctc gagatccatg tcgtgcaggg
     2641 ccacgcccca ctcgaacagg ctcgccagca ggatgttgta gatcggctgg aggaggtaga
     2701 ccgggtgcca cttctgatgc gggtcgatcc gcatgatctc gtagccgagg tccttgtcct
     2761 tgccgcggat gttcgtgtac gtgtggtgga tgtagttgtg ggagtgcttc cacgcctcgg
     2821 aggtcgacgc ggtgtcccag tcccacgtcg tcgagtggat gtagggatcg ttcatccagt
     2881 cccactgccc gtgcaggacg ttgtggccga tctccatgtt ctcgaggatc ttcgcgctcg
     2941 agttggccac cgtgccgaga atccacgccg gcttgaagcg ggacgcgagc agcagcacgc
     3001 gcg
//
LOCUS       DASVPL010000300        13908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_75515, whole genome shotgun sequence.
ACCESSION   DASVPL010000300 DASVPL010000000
VERSION     DASVPL010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13908
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75515"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1344
                     /locus_tag="VF517_17010"
     CDS             34..1344
                     /locus_tag="VF517_17010"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8054690.1"
                     /translation="MPERAQLEQRSVDLLRKLIATNTVNPPGNEEAVQVQLRDLLEAA
                     GFECELLAAVEGRPNLVARLRGGSDGPRLCYLGHADTVLADARDWSVDPWSGELRDGW
                     VWGRGAVDMKGQVAAEVAAVTALAEEGWRPASGELLVVITADEEAGATHGARWLCETH
                     PDKVRCDYVVNEGGGHAFEFEGRPVYTVEVAEKGTFRFTLTTNGHAGHGSIPKIADNA
                     LTKMAPILTAFDERRAELDPSPEPIALMEVLGIDPSDLEAALKEACERDPRMALLLEP
                     TLSVTFAPTMMRASEKINVIPGHAELRVDCRVPPGLGADHARGRLEQVLGDTDGYDVR
                     FDEEVVGNRSRIDSPLMDRIRAFVEREEPGAAVAPVILPGFTDSHWFRKAFPDCVAYG
                     FCPHRKIDLFMAAALMHSADERVPVEDLGLAASFFAELAPELLG"
     gene            1341..2177
                     /locus_tag="VF517_17015"
     CDS             1341..2177
                     /locus_tag="VF517_17015"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX8054691.1"
                     /translation="MTAPVPEDKLRLGGMALRNGLLVHGPTHWAAAVRTRGGEIKVAS
                     GRKPAIGASVGVPGVRGIAKLAEAFAVIPLVKRALPEARLPMQDPKTIGTMAAAAAAG
                     QGLRRAGTRTLARETVIAGISLLPALLALRGGDLASYHGVEHKAIAAYEQDDDAAHAQ
                     KEHDRCGSNLVAPMLASSVAGSVAVRRAGIQGPAADGAVALGSMAIAIELFGWSERHP
                     DSRLTRALRRPGYEIQRAVGTREPSHEQLEVGRAALEEILRVEGNRAIPGSSGDAVPS
                     EG"
     gene            2211..4106
                     /locus_tag="VF517_17020"
     CDS             2211..4106
                     /locus_tag="VF517_17020"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8054692.1"
                     /translation="MRCRLHLNALRALTVAAIAFAFAAPGTASATSYCVAPATGCEHS
                     FKTYNEAVDAADKSPGLDAIRQEKNGTTSERNFSDPSSPAATPAAPQPAANPGVSKPS
                     FFDNFKTWLLTWMPLIFMGLIAVVLGFTLKSMPRTKPQEIKPEGNGSILWEDVAGADE
                     AKAELREVVDFLRDPKRFKKLGAKVPKGILLHGPPGTGKTLLAKAVANEAKAKFYAQS
                     AASFVEMFAGLGAARIRRLFRVARKAAPAIIFIDELDAVGATRGKDVSGEKDQTLNQL
                     LVEMDGFGSTDDVVVIAASNLLDKLDPALLRPGRFDRQILVSPPDLKGRIEILKVHTA
                     NKPVRGVDFDIVARQTSGLTGAELANICNEAAIFAGRLQRDHIVTEDFEKALERVVAG
                     MESRKLMNDHEKRVIAYHEAGHALAAELLDSQEKTHRISIIPRGKALGYTLHLPSEDR
                     YLKTRDEMLDWMVVGLAGRVAEAIVFGSITTGAANDLEKVYSVSRSMVTDYGMGTSIT
                     SRRLPVDDYSVSEHSRRIVDEEQQEMTDRAYRRARDMILANRPLLNAFAEHLLTQEVL
                     ERDDIERLVQLHLEGKLEDAHDVSEPDAVIEGAVVRELEPGALGLAAAERFEADPEPP
                     SSPDDAE"
     gene            4130..4537
                     /gene="mce"
                     /locus_tag="VF517_17025"
     CDS             4130..4537
                     /gene="mce"
                     /locus_tag="VF517_17025"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX8054693.1"
                     /translation="MLFGQIDHIGVVVPDLDEAADLYRSQFQMAEQHRETVEEQGVEA
                     MLLEVGAQHVELIAPLDPESGVGKFLAKNGPGFHHVAYACEDIDSALDTVRDSGLRVI
                     DKKPRRGIRGSRVAFLHPKATGGVLTELVEPAH"
     gene            4537..4776
                     /locus_tag="VF517_17030"
     CDS             4537..4776
                     /locus_tag="VF517_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054694.1"
                     /translation="MEASGKPRRVDIGFNGGQAFSVRLAEAEYRALREALESDRSERW
                     HSISSDDSEVLIDLAQVVYVKLDTEQRGVGFSGPP"
     gene            4804..5079
                     /locus_tag="VF517_17035"
     CDS             4804..5079
                     /locus_tag="VF517_17035"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX8054695.1"
                     /translation="MCRNIRQLHNFDPPASEDEIRDAALQYVRKISGSTKPSKANEEA
                     FNRAVDAVTAASAELLASLTTNAPPKDRETEAEKARARFQKRQAAAA"
     gene            complement(5092..6396)
                     /locus_tag="VF517_17040"
     CDS             complement(5092..6396)
                     /locus_tag="VF517_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8054696.1"
                     /translation="MKHVQLAAVVAAVSLALPASASASKVDLLLGGNEDPSTIEYTAA
                     AGEQNKLDVKVAADGRSAEISDPGAGTVTPGDNCTAQNAKKVTCTVTGTNPKLLTVYA
                     TLLDGNDTFDITGSGSSVNGGDGNDELRGGPLDDGLRGGGGTDELRGNAGSDALYDGD
                     TSGAANKDTLDGGPGDDTVVYERTAGVVVDLADPAGDGEPGENDALVAIENVGGGSGN
                     DQLRGDAGPNRLGGALGDDTIDGRAGDDWVTGFEGNDTLIGGPGRDDIEGGDGDDTLR
                     LENPAGQYDRLLTCSAGKDTIVGITAAPSVEAGCETGDFGFGFVAGLRPKKVTTSSVT
                     IKIPCPDAYRAGSACKGSVVVEPKGAYAKDAATRAKQRYGVKKFSIGGSSAKVTIALN
                     SAGRKQLKKSAFKLQFSIRMKETATKTKRQFEWTSYVVRGTR"
     gene            complement(6487..6702)
                     /locus_tag="VF517_17045"
     CDS             complement(6487..6702)
                     /locus_tag="VF517_17045"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX8054697.1"
                     /translation="MAESVYRVTEVIGVSTESWDAAARNAVETAGKSVRDLRIAEVIR
                     QDVSIQDGKVANFRVRLAISFKYESGE"
     gene            6770..7876
                     /locus_tag="VF517_17050"
     CDS             6770..7876
                     /locus_tag="VF517_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8054698.1"
                     /translation="MRMRAAVLEEFGEPLVVDELELADPRAGEVLVRLGACGVCHTDM
                     YTWSGVDPSGYAPTVLGHEGAGVVEAVGEGVSSLAVGDHVVTLFSPQCRECENCRSGK
                     TNICTAIREEQNRGFLPDGTARLSRGGEPIRHFMGTSTFAEYTVMPEIALAKVSPEAP
                     LEAACLFACGLSTGLGAAMYKAEVEAGSTCVVFGAGMVGLGAVVGCRLQGAARIVCVD
                     MSEERLALARRHGATETWTGGEHVVERVLEETGGMGADYTFEATGLVGVMRQAVEAAR
                     MGWGLATIAGVAGRGEVLEVVPRYLITGRRVIGASFGGVKGRDQVPELVDRYMAGDID
                     VDSFISHRMPLDDVNRGFELMEAQDGIRSVIRFD"
     gene            8200..8514
                     /locus_tag="VF517_17055"
     CDS             8200..8514
                     /locus_tag="VF517_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054699.1"
                     /translation="MARVSRAPRSDGPRTTLRLTPAIVETAERLAGELGVSRNDALLR
                     LAARGAHLYEEERRIAERRDERWAAVVAAGADAGQAGFPSADEAWDAVALAREGFDAP
                     EA"
     gene            8530..9045
                     /locus_tag="VF517_17060"
     CDS             8530..9045
                     /locus_tag="VF517_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054700.1"
                     /translation="MHFISMPAAWDTTLLSRVQPAGDAVEYVLGRAAAGEPVRVAAPA
                     VLEVTYGYELRAGRDPRFRSLLDWFTHLMASGTFRVVPLDGRAAVVAGRVRAAMPHPP
                     GGRKRDPRSKTMRQAAWLLDIQIAASAFAAGLDVATANRADFDEIAAVLATLFPAAPR
                     LEVTDEPEGSA"
     gene            complement(9042..9698)
                     /locus_tag="VF517_17065"
     CDS             complement(9042..9698)
                     /locus_tag="VF517_17065"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HEX8054701.1"
                     /translation="MSDELRDRLRALRVFEGDLPEFEPGETPEEPVPLFLAWLDDAIE
                     RDVRAPHAMTLANLDGYGRPDSRVLLLKGVAGGRLEFATSRTSRKGRGLTLTPWAAAT
                     FHWPEIARQVRVRGRVADAGREAAARDFLARPKESRAESLHGRQSERLDDPAKLDRAF
                     EEGLERVAAEPELVPDHWVVYQLVPDEIEFWQGRPDRRHVRLRYRHDGERWTRALLWP
                     "
     gene            complement(9695..10507)
                     /locus_tag="VF517_17070"
     CDS             complement(9695..10507)
                     /locus_tag="VF517_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054702.1"
                     /translation="MARDVQRPPTRAELNRLLIGNAVTKTWPNVLLPAGLAVAGLLIG
                     LNIAVLLPVAIVAWLALSAVTYFDTDEAEAVAQRDRARRRGALDTGRRTDLRALAPPV
                     AAHLQQVLTQEAHIRGAIERADLPFGEVSDEVDTFVRAAEKTAGRAQLLYEYLAEQDP
                     RQVEYRYQQVRAEADPSKKALAEALWTQLQAMNRAHKELESFYTEMERIAVELGNIRG
                     QLLSVSAASEGASQRQLASDVRDLREQMGAVAEGMNEALAETGAAPSAAGPA"
     gene            complement(10507..12201)
                     /locus_tag="VF517_17075"
     CDS             complement(10507..12201)
                     /locus_tag="VF517_17075"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX8054703.1"
                     /translation="MKRSQAIIAAIAVVAVIALAFGRGGGDDGGGGGGNGKAPSKAPS
                     GSVAVSFAYSPEKEKLLEPLIDRFNGERKKVGGKQVFVEGQVVSSGEAQSKIARGTLK
                     PVAWSPASSLWGRLLNFEADRPYAPADNPSIVRTPLVIAMWEPMAQALGYPRKKIGFD
                     DILGLARSDRGWADFGRPQYGDFKLVHTNPDFSTSGLSAVVAEYYAATGKKEGLVEKD
                     IQGSRARAIVKDIERSIVHYGDTTLFIADQMRKAGPGYASAVAMEEVTLLDFNKNRGG
                     QPKLVAIYPEEGTFYSDNPFIVLDAPWVNPQQRDGAREFAKFLEEEIDAERAGESGFR
                     PPDLKEKPAAAISAANGADPAQPARVLGLPEPRVLSHVKAAWREDRKPANVLLVVDTS
                     GSMVEENRLERAKEGLKAFLREVSPNDRVGLTAFSDEINPLVPIAPVRQNESKLRSTI
                     DTLIADGGTAFHDAAIRGLADVQQLRDRDRINAVVLLTDGEDTDSSATIDQAVQRMEA
                     QGDTENPVRVFTIAYSAGAEGAEEGLKRLAEGSGGQAYSGDTEDIELVYRSISSFF"
     gene            12253..12738
                     /locus_tag="VF517_17080"
     CDS             12253..12738
                     /locus_tag="VF517_17080"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8054704.1"
                     /translation="MPAALDTALLRLLRAHGHSPRVERAVSAFSKLGEHAAIWLGLAA
                     AGAALDRRRRDEFVRAGRTVVLSYVVNQAIKQTVRRPRPDIEGLPQLVGTPTQLSYPS
                     THACTSFAGAGALSGLLPAAPLYVLAVPLALSRLYLGVHYPSDTLAGGVVGAAVAELA
                     R"
     gene            12735..13817
                     /gene="ychF"
                     /locus_tag="VF517_17085"
     CDS             12735..13817
                     /gene="ychF"
                     /locus_tag="VF517_17085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX8054705.1"
                     /translation="MRIGIVGLPNAGKSSLFNALTRAGIEAAPYPFTTVEPNVAIVPV
                     PDERLDRVAETVSAKPVVHETIAFHDIAGLVRGAHQGEGLGNRFLANIRETDAIVHVV
                     RAHDDEQVPHPEGRVDPAGDIEAIETELLYADLEQVERRLERVTKQARSLDKVAIAEE
                     AWLREVVEALQAGRPVRSVPAPDDAPDAVRSLQPLTSKPVLYLANVGEDAGDEVPAAV
                     AERAAEAGAGAVAVSARLESELTELDPDEAAAMREDLGVPESGLARVVHAAFSLLELI
                     SFFTAGQDKEARAHAIRDGTTAWEAAGQIHSDIQRGFVRAEVVGWEELVAAGGYSGAR
                     DKGQLRSEGRDYRMRDGDVFHVKFTP"
BASE COUNT         1864 a         5101 c         4922 g         2021 t
ORIGIN      
        1 gctgttggtc cacgggcata tagtggcggc accgtgcccg agcgcgctca actggagcaa
       61 cgcagcgtcg atctgctgcg caagctgatc gccacgaaca ccgtcaatcc gcccgggaac
      121 gaggaggcgg tgcaggtcca gctacgcgac ctgctcgagg cagcgggctt cgaatgcgag
      181 ctgctggcgg cggtcgaggg gcggccgaac ctcgtcgcgc gcctgcgcgg gggctcggac
      241 ggcccgcggc tctgctacct cggccacgcc gacaccgtgc tcgccgacgc gcgcgactgg
      301 agtgtggacc cgtggtcggg cgagctgcgc gacggctggg tctgggggcg cggcgcggtg
      361 gacatgaagg gccaggtggc ggccgaggtc gcggcggtca cggcgctcgc cgaggagggc
      421 tggcgcccgg cgtcgggcga gctgctcgtg gtgatcaccg ccgacgagga ggccggcgcc
      481 acgcacggcg cgcgctggct ctgcgagacg caccccgaca aggtccgctg cgattacgtg
      541 gtcaacgagg gcggcgggca cgcgttcgag ttcgagggcc ggcccgtgta cacggtcgag
      601 gtggcggaga agggcacctt ccggttcacg ctcaccacca acgggcacgc cggccacggc
      661 tcgatcccga agatcgccga caacgcgctc accaagatgg ccccgatcct cacggccttc
      721 gacgagcggc gcgcggagct cgacccgtcg cccgagccga tcgcgctgat ggaggtgctg
      781 gggatcgacc cgagcgatct cgaggccgcg ctgaaggagg cctgcgagcg tgacccgcgc
      841 atggcgctgc tgctcgagcc gacgctgagc gtgaccttcg cgccgacgat gatgcgcgcc
      901 tccgagaaga tcaacgtgat ccccggccac gcggagctgc gcgtggactg ccgcgtaccg
      961 ccagggctcg gcgccgacca cgcccgcggg cgactcgagc aggtgctggg cgatacagac
     1021 ggctacgacg tgcgcttcga cgaggaggtg gtcggcaacc gctcgcggat cgactccccg
     1081 ctgatggatc gcatccgcgc cttcgtggag cgcgaggagc cgggcgccgc ggtcgcgccg
     1141 gtgatcctgc ccggcttcac cgactcccac tggttccgca aggcgttccc cgactgcgtg
     1201 gcgtacggct tctgcccgca tcgcaagatc gacctgttca tggccgccgc gctcatgcac
     1261 agcgccgacg agcgcgtacc cgtggaggac ctcggcctgg ccgcgagctt cttcgcggag
     1321 ctggctccgg agctgctcgg atgaccgcgc cggtacccga ggacaagctg cgcctcggcg
     1381 gcatggcgct tcgcaacggg ctgctggtcc acgggccgac gcactgggcc gccgcggtgc
     1441 gcacgcgcgg cggcgagatc aaggtcgcgt cggggcgcaa gcccgccatc ggggcgagcg
     1501 tcggcgtgcc cggcgtgcgc gggatcgcga agctcgccga ggcgttcgcg gtgatccccc
     1561 tcgtcaagcg cgccctgccc gaggcgcggc tgccgatgca ggacccgaag acgatcggga
     1621 cgatggcggc cgccgccgcg gccggccagg gattgaggcg cgcgggcacg cgcacgctcg
     1681 cgcgcgagac ggtcatcgct gggatcagcc tgctgcccgc gctgctggcg ctgcgcggcg
     1741 gcgacctcgc ctcctaccac ggggtcgagc acaaggcgat cgccgcctac gagcaggacg
     1801 acgacgccgc gcacgcccag aaggagcacg accgctgcgg ctcgaacctc gtggccccga
     1861 tgctcgcctc gtcggtcgcc ggcagcgtgg cggtgcgccg ggccggcatc cagggccccg
     1921 ccgccgacgg cgcggtggcg ctcggcagca tggcgatcgc gatcgagctg ttcggctggt
     1981 ccgagcgcca tccggactcg cgcctcaccc gcgcgctgcg ccgcccgggg tacgagatcc
     2041 agcgcgccgt gggcacccgc gagccctcgc acgagcagct cgaggtgggc cgggccgcgc
     2101 tggaggagat cctgcgggtg gaggggaatc gcgccatccc gggaagttcc ggggatgccg
     2161 tgccctccga gggctaaagc ccccgcccgc atcgggcgat aggaccgcag atgcgctgcc
     2221 gtctgcacct gaatgccctc cgcgccctga ccgtcgccgc gatcgccttc gcgttcgccg
     2281 cgcccggcac cgcctcggcc accagctact gcgtcgcccc cgcgaccggc tgcgagcaca
     2341 gcttcaagac ctacaacgag gcggtcgacg ccgccgacaa gagccccggc ctcgacgcga
     2401 tccgccagga gaagaacggg accacgtcgg agcgcaactt cagcgaccca tcgtccccgg
     2461 ccgccactcc cgccgcgccg cagcccgccg cgaacccggg cgtctccaag cccagcttct
     2521 tcgacaactt caagacctgg ctcctcacct ggatgcctct gatcttcatg ggcctcatcg
     2581 ccgtcgtgct cggcttcacc ctgaagagca tgccgcgcac caagccgcag gaaatcaagc
     2641 cggagggcaa cggctcgatc ctctgggagg acgtggccgg cgccgacgag gccaaggccg
     2701 agctgcgcga ggtcgtcgac ttcctgcgcg accccaagcg cttcaagaag ctcggcgcca
     2761 aggtgcctaa gggcatcctg ctgcacggcc cccccggcac cggcaagacg ctgctcgcca
     2821 aggccgtcgc caacgaggcc aaggccaagt tctacgccca gtccgccgcc tcgttcgtcg
     2881 agatgttcgc cggcctcggc gcagcccgca tccggcgctt gttccgcgtg gcccgcaagg
     2941 ccgccccggc gatcatcttc atcgacgagc tcgacgctgt cggcgccacg cgcggcaagg
     3001 acgtctcggg cgagaaggac cagacgctca accagctcct cgtcgagatg gacggcttcg
     3061 gctcgaccga cgacgtggtc gtgatcgcgg cctcgaacct gctcgacaag ctcgacccgg
     3121 cgctgctgcg ccccggccgc ttcgaccgcc agatcctcgt ctcgccgccc gacctcaagg
     3181 gccgcatcga gatcctcaag gtgcacacgg cgaacaagcc cgtgcgcggc gtcgacttcg
     3241 acatcgtcgc gcgccagacc agcggcctga ccggcgccga gctggccaac atctgcaacg
     3301 aggccgcgat cttcgccggc cgcctccagc gcgaccacat cgtcaccgag gacttcgaga
     3361 aggcgctcga gcgggtcgtc gccggcatgg agtcgcgcaa gctgatgaac gaccacgaga
     3421 agcgcgtgat cgcctaccac gaggccgggc acgcactcgc cgcggagctg ctcgactcgc
     3481 aggagaagac gcaccgcatc tcgatcatcc cgcgcggcaa ggcgctcggc tacacgctgc
     3541 acctgccgtc cgaggaccgc tacctgaaga cgcgcgacga gatgctcgat tggatggtcg
     3601 tgggcctcgc cggccgcgtg gccgaggcga tcgtgttcgg ctcgatcacc accggcgccg
     3661 ccaacgacct cgagaaggtc tactcggtat cgcgctcgat ggtcacggac tacggcatgg
     3721 gcacgtcgat cacctcgcgc cgcctcccgg tggacgacta ctcggtctcc gagcacagcc
     3781 gccggatcgt ggacgaggag cagcaggaga tgaccgaccg ggcctaccgc cgcgcccgcg
     3841 acatgatcct ggccaaccgc ccgctgctga acgccttcgc cgagcacctg ctcacgcagg
     3901 aggtgctgga gcgcgacgac atcgagcggc tcgtgcagct gcacctcgag ggcaagctcg
     3961 aggacgcgca cgacgtgtcc gagcccgatg ccgtgatcga gggcgccgtt gtacgcgagc
     4021 tcgagcccgg tgcgctcggc ctcgccgccg ccgagcgctt cgaggcggac cccgagccgc
     4081 ccagctcgcc cgacgacgcc gagtaggtcg cctcgtacac tccctgcccg tgctgtttgg
     4141 gcagatcgac cacatcggcg ttgtagtccc cgacctcgac gaggccgcgg acctctaccg
     4201 ctcgcagttc cagatggcgg agcagcaccg cgagaccgtc gaggagcagg gcgtcgaggc
     4261 catgctgctc gaggttggag cccagcacgt ggagctgatc gcgccgctcg acccggagtc
     4321 gggcgtcggc aagttcctcg cgaagaacgg cccgggcttc catcacgtcg cctacgcgtg
     4381 cgaggacatc gactccgcgc tcgacaccgt gcgtgattcc ggcctgcggg tgatcgacaa
     4441 gaagccgcgc cgcggcatcc gcggcagccg ggtcgctttc ctgcacccga aggccaccgg
     4501 cggcgtgctc accgagctcg tggagccggc gcactgatgg aagcatcggg caagccgcgc
     4561 cgcgtcgaca tcggcttcaa cggcgggcag gcgttctccg tccgcctcgc cgaggccgag
     4621 taccgcgccc tgcgcgaggc gcttgagtcc gaccgcagcg agcgctggca ctccatttcg
     4681 agcgacgact ccgaggtgct gatcgacctc gcccaggtgg tctacgtcaa gctcgacacc
     4741 gagcagcgcg gcgtgggctt ctccggccct ccctgatcgc agcgcgcggc tacgctgtcg
     4801 ggcatgtgcc gaaacatccg acagctccat aacttcgatc cacccgccag cgaggacgag
     4861 atccgcgacg cggcccttca gtacgtccgc aagatcagcg gctcgaccaa gccttcgaag
     4921 gccaacgagg aggcgttcaa ccgcgccgtg gacgccgtga cggcggcctc ggccgagctc
     4981 ctcgcctcgc tgacgaccaa cgctcccccg aaggatcggg agaccgaggc cgagaaggcg
     5041 cgggctcgct tccagaagcg ccaggccgcc gccgcttagc gggtccgccg cttagcgggt
     5101 cccccgcacc acgtaagagg tccactcgaa ctggcgcttg gtcttcgtcg cggtctcctt
     5161 catccggatc gagaactgga gcttgaacgc ggacttcttg agctgcttgc ggccggctga
     5221 gttgagcgcg atcgtgacct tcgcgctcga gccgccgatc gagaacttct tcactccgta
     5281 gcgctgcttg gcccgcgtcg ccgcatcctt cgcgtaggcg cccttcggct cgaccacaac
     5341 cgagcccttg caggcactcc ccgcccggta ggcgtccggg cacgggatct tgatcgtcac
     5401 cgagctggtc gtgaccttct tcggccgcag gccggcgacg aacccgaacc cgaagtcgcc
     5461 ggtctcgcag ccggcctcca cgctcggcgc cgccgtgatc ccgacgatcg tgtccttgcc
     5521 ggcgctgcac gtgagcagcc ggtcgtactg gccggccggg ttctcgagcc ggagcgtgtc
     5581 gtcgccgtcg ccgccctcga tgtcgtcgcg tcccgggccg ccgatcagcg tgtcgttgcc
     5641 ctcgaaaccg gtgacccagt cgtcgcccgc gcggccgtcg atcgtgtcgt cgcccagcgc
     5701 gccgccgagg cggttggggc ccgcgtcgcc gcggagctgg tcgttcccgg agccgccacc
     5761 cacgttctcg atcgcgacga gcgcgtcgtt ctcgcccggc tcgccgtcgc ccgccgggtc
     5821 ggcgaggtcg accaccacgc cggcggtgcg ctcgtagacg acggtgtcgt cgccggggcc
     5881 gccgtcgagc gtgtccttgt tcgcggcgcc ggaggtgtcg ccgtcgtaga gagcgtccga
     5941 gccggcgttg ccgcgcagct cgtcggtgcc gccgccaccg cggaggccgt cgtcgagcgg
     6001 gccgccgcgc agctcgtcgt tgccgtcacc gccgttcaca ctgctgcccg agcccgtgat
     6061 gtcgaacgtg tcgttgccgt cgagcagcgt cgcgtagacg gtcaggagct tcgggttcgt
     6121 gcccgtgacc gtgcacgtca ccttcttcgc gttctgcgcc gtgcagttgt cgccgggcgt
     6181 gaccgtgccg gcgccggggt cgctgatctc ggcgctcctg ccgtccgccg cgaccttcac
     6241 gtcgagcttg ttctgctcgc ccgccgccgc cgtgtactcg atggtcgacg gatcctcgtt
     6301 gccgcccagc agcaggtcga ccttcgatgc cgatgcgctg gccggcagcg cgagagagac
     6361 cgcggccacg accgcggcga gctgaacgtg cttcatgttg gcccctgtcg ttcgtgaagt
     6421 tcctacccgt accgacccgc gagcggcccc gctgttgcgc gcggccgccc gtaagtcggc
     6481 atgttgctac tcgcccgact cgtacttgaa cgagatcgcg aggcgcaccc ggaagttcgc
     6541 gacctttccg tcctggatcg agacgtcctg acggatgacc tcggcgatgc gcaggtcgcg
     6601 cacgctcttg ccggccgtct ccacggcgtt cctcgcggcc gcgtcccagg actccgtgct
     6661 caccccgatc acctcggtta ctcggtagac gctctcggcc atgtgtacct ccatcgttgc
     6721 tgacgttggc gcaaccctct catgtttccg gtgtgtaggg tcggttgcga tgcgcatgcg
     6781 cgccgccgtg ctggaggagt tcggggagcc gctcgtcgtg gacgagctgg agctggccga
     6841 cccgcgggcc ggcgaggtgc tcgtgcggct cggcgcctgc ggcgtctgcc acaccgacat
     6901 gtacacctgg tcgggggtgg acccgtccgg ctacgcgccg accgtgctgg gccacgaggg
     6961 cgccggcgtg gtggaggcgg tgggcgaggg cgtatcgtcg ctcgcggtcg gcgaccacgt
     7021 ggtcacgctg ttctcgccgc agtgccgcga gtgcgagaac tgccgcagcg gcaagaccaa
     7081 catctgcacc gcgatacgcg aggagcagaa ccggggcttc ctgcccgacg gcaccgcccg
     7141 gctctcgcgc ggcggcgagc cgatccgcca cttcatgggc acctccacct tcgcggagta
     7201 cacggtgatg ccggagatcg cgctggcgaa ggtctcgccg gaggcgcccc tggaggccgc
     7261 gtgcctgttc gcctgcggac tctcgaccgg cctcggcgcg gccatgtaca aggccgaggt
     7321 cgaggccggg tcgacctgcg tggtgttcgg tgcggggatg gtcgggctcg gcgcggtggt
     7381 cggctgccgg ctccaggggg cggcgcggat cgtctgcgtg gacatgagcg aggagcggct
     7441 cgcactcgcg cgccggcacg gcgcgaccga gacgtggacc ggcggcgagc acgtggtcga
     7501 gcgggtgctc gaggagacgg gcgggatggg cgccgactac accttcgagg cgaccgggct
     7561 tgtcggcgtg atgcgccagg ccgtcgaggc cgcgcgcatg ggctgggggc tcgcgacgat
     7621 cgcgggcgtg gccggccgcg gcgaggtgct ggaggtcgtg ccgcgctacc tgatcaccgg
     7681 gcggcgcgtg atcggcgcgt ccttcggcgg cgtgaagggc cgcgaccagg tgcccgagct
     7741 ggtcgaccgc tacatggccg gcgacatcga cgtggacagc ttcatctcac accggatgcc
     7801 gctcgacgac gtgaatcgcg gcttcgagct gatggaggcc caggacggca tccgcagcgt
     7861 gatccgcttc gactgagggc tcgcgggccg ggcgggccgg cggagctact tcgcgcggcc
     7921 cttgcgcggg cggttctgcg gcgggttggc cttgcgctcc tcaggcgtca tctgcctgat
     7981 ctgaacggtg ccctcgtcga cctggcggtc gaactcggcc acgctcttct cacgcttgat
     8041 gtcctgctgt cgccgcgtgg gcgacggcgg ctccttggcc ttcttgtcgt ccggcttctt
     8101 gtctgtcggg gcgtcttcgg cgatacagcc agcctatcga cccgccgagt cgtctcgtgg
     8161 ccgcattgaa cgcatcactt gatgcgttag cctgtgggca tggcacgagt cagccgagcg
     8221 ccacgcagcg acgggccgcg gacgacactg cggctgacgc cggccatcgt cgagaccgcc
     8281 gagcggctcg ccggcgagct cggggtatca cgcaacgacg cactgctgcg cctggccgcg
     8341 cgcggcgcgc acctgtacga ggaggagcgc cgcatcgccg agcgccgcga cgagcgctgg
     8401 gccgcggtgg ttgcggccgg cgccgatgct ggccaggcgg gcttcccgtc cgcggacgag
     8461 gcgtgggacg cggtcgctct cgcgcgcgaa gggttcgacg cgccggaggc ctagcgggcc
     8521 cgggcgcccg tgcacttcat ctcgatgccc gcggcgtggg acacgaccct gctctcccgc
     8581 gtccaaccgg ccggggacgc cgtcgagtac gtcctcggcc gggcggcggc gggcgagccg
     8641 gtccgcgtcg ccgctcccgc cgtcctcgag gtgacctacg gctacgagct gagggctgga
     8701 cgcgatccgc gctttcgcag cctgctcgac tggttcacac acctcatggc gtcgggaacg
     8761 ttccgcgtcg tcccactcga cggccgggcg gcggtggtcg cggggcgcgt ccgagcggcc
     8821 atgccgcacc cacccggcgg caggaagcgc gacccgcgca gcaagaccat gcggcaggcc
     8881 gcctggctcc tcgacatcca gattgcggcg agcgcgttcg ccgcggggct cgacgtggcc
     8941 acggcgaacc gcgccgactt cgacgagatc gccgcggtgc tcgcgacgct gtttccggcc
     9001 gctccacgac tcgaggtcac ggacgagccg gagggatctg cctagggcca gagcagcgcg
     9061 cgcgtccagc gctcgccgtc gtggcggtag cgcaggcgga cgtggcggcg gtccggccgg
     9121 ccctgccaga actcgatctc gtcgggcacg agctggtaga cgacccagtg gtccgggacc
     9181 agctccggct cggccgccac gcgctccagc ccctcctcga acgccctgtc gagcttggcg
     9241 ggatcgtcca gccgctcgct ctggcggccg tggagcgatt cggcgcggga ttcctttggg
     9301 cgcgctagga agtcgcgcgc cgccgcctcg cgcccggcgt cggccacgcg gccgcgcacg
     9361 cggacctggc gggcgatctc gggccagtgg aacgtggccg cggcccacgg cgtcagggtc
     9421 agcccgcggc ccttgcggct cgtgcggctg gtggcgaact cgagccgccc gccggccacg
     9481 cccttcagca gcagcacgcg cgagtccggc cggccgtagc cgtcgaggtt cgcgagcgtc
     9541 atggcgtgcg gcgcgcgcac gtcgcgctcg atcgcgtcgt cgagccaggc gaggaacagc
     9601 gggacgggct cctccggcgt ctcacccggc tcgaactccg gcaggtcgcc ctcgaacacg
     9661 cgcagagcgc gcagccggtc gcgcagctcg tcgctcatgc cggccccgcc gcgctcggtg
     9721 ccgcgcctgt ctccgccagc gcctcgttca tgccctcggc caccgcgccc atctgctcgc
     9781 gcaggtcgcg cacgtcgctc gcgagctgtc gctgcgaggc gccctcgctc gccgccgaca
     9841 ccgacagcag ctggcccctg atgttgccga gctccaccgc gatgcgctcc atctccgtgt
     9901 agaaggactc gagctccttg tgggcccggt tcatcgcctg gagctgcgtc cagagcgcct
     9961 cggcgagcgc cttcttcgac gggtccgcct cggcgcgcac ctgctggtag cggtactcga
    10021 cctgtcgcgg gtcctgctcg gcgaggtact cgtagaggag ctgcgcgcgg ccggcggtct
    10081 tctccgcggc gcgcacgaag gtgtcgacct cgtccgagac ctcaccgaac gggaggtcgg
    10141 cccgctcgat cgccccgcgg atgtgagcct cctgcgtgag cacctgctgg aggtgcgcgg
    10201 cgaccggcgg cgcgagtgcc ctcaggtcgg ttcgcctgcc cgtgtcgagg gcgccgcgcc
    10261 ggcgggcgcg gtcgcgctgc gcgacggcct cggcctcgtc cgtgtcgaag taggtgaccg
    10321 ccgagagcgc cagccacgcc acgatcgcca ccggcagcag gaccgcgatg ttcaggccga
    10381 tcagcagccc ggcgaccgcg agcccggccg gcaggagcac gttcggccac gtcttcgtga
    10441 ccgcgttccc gatcaggagc cggttgagct cggcccgcgt gggcggccgc tggacgtcgc
    10501 gagccatcag aagaacgacg agatgctccg gtagacgagc tcgatgtcct cggtgtcgcc
    10561 cgagtaggcc tggccgccgg agccctcggc gagccgcttc agcccctcct ccgccccctc
    10621 ggccccggcc gagtacgcga tcgtgaacac gcgcacgggg ttctccgtgt cgccctgggc
    10681 ctccatgcgc tgcaccgctt ggtcgatcgt cgccgacgag tccgtgtcct cgccgtcggt
    10741 gagcagcacc accgcgttga tgcgatcgcg gtcgcgcagc tgctgcacgt cggcgagccc
    10801 gcgtatggcc gcgtcgtgga acgcggtccc accgtccgcg atcagcgtgt cgatcgtcga
    10861 gcgcagcttc gactcgttct gccgcaccgg cgcgatgggc acgagcgggt tgatctcgtc
    10921 ggagaacgcg gtcagtccga cgcggtcgtt cggcgagacc tcgcgcagga aggccttcag
    10981 cccctccttc gcgcgctcga ggcggttctc ctcgaccatc gagccggagg tgtccaccac
    11041 aagcagcacg ttcgccggct tgcggtcctc gcgccaggcc gccttcacgt ggctcagcac
    11101 gcgcggctcg ggcagcccga gcacgcgcgc aggctgcgcc gggtccgcgc cgttcgcggc
    11161 ggagatcgcg gcggccggct tctccttcag gtccggcggg cggaacccgg actcgccggc
    11221 gcgctcggcg tcgatctcct cctcgaggaa cttcgcgaac tcgcgcgcgc cgtcgcgctg
    11281 ctgcgggttc acccacggcg cgtcgagcac gatgaacggg ttgtccgagt agaacgtccc
    11341 ctcctcggga tagatcgcga cgagcttcgg ctggccgccg cggttcttgt tgaagtcgag
    11401 cagcgtcacc tcctccatcg cgaccgcgga cgcgtagccc ggacccgcct tgcgcatctg
    11461 gtcggcgatg aacagcgtgg tgtcgccgta gtggacgatc gagcgctcga tgtccttgac
    11521 gatcgcccgc gcccgcgagc cctggatgtc cttctcgacc agcccctcct tcttgcccgt
    11581 ggcggcgtag tactcggcca ccaccgccga gagcccggag gtggagaagt cggggttcgt
    11641 gtggacgagc ttgaagtcgc cgtactgcgg gcggccgaag tcggcccagc cgcggtcgga
    11701 gcgcgcgagc ccgaggatgt cgtcgaagcc gatcttcttg cgcgggtagc cgagcgcctg
    11761 cgccatcggc tcccacatcg cgatcacgag cggcgtgcgc acgatcgacg ggttgtccgc
    11821 gggcgcgtag gggcggtcgg cctcgaagtt caggaggcgt ccccagagcg acgaggcggg
    11881 cgaccaggcg accggcttga gcgtgccgcg cgcgatcttc gactgcgcct cgcccgacga
    11941 gacgacctgt ccctccacga acacctgctt gccgccgacc ttcttgcgct cgccgttgaa
    12001 gcggtcgatc agcggctcga gcagcttctc cttctcgggc gagtaggcga acgagaccgc
    12061 caccgagccg gagggcgcct tgctgggggc cttgccgttc ccgccgccgc caccgccgtc
    12121 gtccccgccg ccgcgcccga acgcgagggc gatgacggcg accaccgcga tcgcggcgat
    12181 gatggcctgg ctgcgcttca cgggagcatc gtacgggggc cgcccgaccg gccgacccta
    12241 tcctcggccg ccgtgcccgc cgccctcgac accgcgctgc tgcgcctgct gcgcgcgcac
    12301 ggccactcgc cgcgcgtcga gcgggccgtg tccgcgttct cgaagctcgg cgagcacgcg
    12361 gccatctggc tcgggctggc cgccgcgggc gcggcgctcg accgccgccg gcgcgacgag
    12421 ttcgtgcgtg cgggtcggac cgtcgtcctc tcctacgtcg tcaaccaggc gatcaagcag
    12481 accgtgcgcc gcccgcggcc ggacatcgag gggctgccgc agctcgtcgg caccccgacg
    12541 cagctctcct acccctcgac gcacgcctgc acgagtttcg cgggagctgg cgccctgagc
    12601 ggcctgctgc cggccgcgcc gctctacgtg ctcgcggtcc cgctcgcgct ctcgcggctc
    12661 tacctcggcg tgcactaccc gtccgacacc ctggccggcg gcgtcgtggg cgcggcggtc
    12721 gcggagctgg cgcgatgagg atcggcatcg tcgggcttcc gaacgcgggc aagtcctcgc
    12781 tcttcaacgc gctcacgcga gctggcatcg aggcggcgcc gtacccgttc accaccgtcg
    12841 agccgaacgt ggcgatcgtg ccggtcccgg acgagcgcct cgaccgggtg gccgagaccg
    12901 tgtcggcgaa gccggtcgtg cacgagacca tcgcgttcca cgacatcgcc gggctcgtgc
    12961 gcggcgccca ccagggcgag gggctcggca accgcttcct cgcgaacatc cgcgagaccg
    13021 acgcgatcgt gcacgtggtg cgcgcccacg acgacgagca ggtgccccac cccgagggcc
    13081 gcgtcgatcc ggccggcgat atcgaggcga tcgagaccga gctgctctac gccgacctcg
    13141 agcaggtcga gcggcggctc gagcgggtca cgaagcaggc gcggtcgctc gacaaggtgg
    13201 cgatcgcgga ggaggcctgg ctgcgcgagg tggtcgaggc gctccaggcg ggcaggccgg
    13261 tgcgctcggt gcccgcgccc gacgacgcgc ccgacgccgt tcgctcgctc cagccgctca
    13321 cctcgaagcc ggtcctctac ctcgccaacg tgggcgagga cgccggcgac gaggtgccgg
    13381 ccgccgtcgc cgagcgggcc gccgaggcgg gagcgggggc cgtggccgtg agcgcgcggc
    13441 tcgagtccga gctgaccgag ctcgaccccg acgaggccgc cgccatgcgc gaggacctcg
    13501 gcgtgcccga gtccgggctc gcgcgcgtgg tgcacgccgc cttctccctg ctcgagctga
    13561 tctcgttctt caccgccggc caggacaagg aggcccgcgc ccacgcgatc cgcgacggca
    13621 ccaccgcctg ggaggccgcg gggcagatcc actccgacat ccagcgcggg ttcgtgcgcg
    13681 cggaggtggt cgggtgggag gagctggtgg ccgcgggtgg ctactccggc gcgcgcgaca
    13741 aggggcagct ccgctccgag ggccgcgact accgcatgcg cgacggcgac gtgttccacg
    13801 tgaagttcac gccgtagcgc gagctagtcg cgttcgaagc gccgcgagcc ggccggctcg
    13861 ggctccgacc cggggccgtc ggcggccacg cccacgggcg tacgctcc
//
LOCUS       DASVPL010000301        20130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_75679, whole genome shotgun sequence.
ACCESSION   DASVPL010000301 DASVPL010000000
VERSION     DASVPL010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 20130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20130
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75679"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VF517_17090"
     CDS             <1..671
                     /locus_tag="VF517_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX8054706.1"
                     /translation="EAYATELGGRLVDQGAKLLVVACNSATAAALPSLRATLGLEVPI
                     VAVVEPDARLAAQVTQNGQVGLLATEATVASRAYDHALAQHAPDATLHAVACPELAPL
                     IQAGEEIDVRVLRLIEGYCDPLRRAGVDTVILGCTHYPLVRPMLQRELGRDVMLVSSA
                     EAVADAVERELDAGGLSAPLDRRGSYRFMCSGDPDEFRRVGTRFLQLPMEDVEHVDVA
                     GVAA"
     gene            668..1405
                     /gene="rph"
                     /locus_tag="VF517_17095"
     CDS             668..1405
                     /gene="rph"
                     /locus_tag="VF517_17095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX8054707.1"
                     /translation="MSGGTRNDGRSAAELRPTTITPDFMRTATGSALIEQGGTRVICT
                     ASAVEDVPRWMRGKGTGWVTAEYGMLPASTGDRKQRDVSKGRPDGRTVEIQRLIGRSI
                     RGIVDFAALGERTIYLDCDVLEADGGTRCASITGAYVALHLACARMVDEKKLSAIPLT
                     QSVAAVSCGMVGGIPLLDLDYSEDSTAEVDANVVMTGDGGLVEVQATAERTPLSRASL
                     DDLLALAAAGIERLRRAQLEAVGAPVA"
     gene            1415..2104
                     /gene="rdgB"
                     /locus_tag="VF517_17100"
     CDS             1415..2104
                     /gene="rdgB"
                     /locus_tag="VF517_17100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX8054708.1"
                     /translation="MQLVLATRNEHKLRELRELLPGVELLALPADVVMPPETGDTFVA
                     NAIIKAEAAVAATGLPAVADDSGIEASALGGAPGVYSARFAGEHASDQENLDKLLREV
                     TPDSDPRLAYACVLAYLPAAGAEPVLFEGRCTGTITHDPRGEHGFGYDPAFVPDDRDD
                     GLTMAELDPRDKDAISHRGRAARAFASWLETAGARAGDGEATDRGGSGGEATDRGADG
                     RATDRGEGEAA"
     gene            2101..2991
                     /locus_tag="VF517_17105"
     CDS             2101..2991
                     /locus_tag="VF517_17105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8054709.1"
                     /translation="MIVAVENAAHADRKTSAARLSIASNAALIVLKLVAGAITGSIAI
                     ITEAVHSSIDLMASIVAYFSIRKAEEPADESHPYGHEKVENLAAALEGVLILVGAGII
                     VFESSRRLVDPPEVESLGFGIAAIAVSVVVNLVVSSYLYRRAKQTHSPALEGDAAHLR
                     TDAVTSVAVLLGLALVAITDTKALDPIVALAVAAAIVWAGVRLITRSSRVLVDEALPE
                     DELAAIALAVESSGAPEVAGFHKLRARRAGSRRYIDLHVQFRPGTTLERAHSVSHELQ
                     ADIRRRVHRADVLIHIEPES"
     gene            complement(2992..3132)
                     /locus_tag="VF517_17110"
     CDS             complement(2992..3132)
                     /locus_tag="VF517_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054710.1"
                     /translation="MPLSRIAWMVTVGICLISAVLVLLSGYVGYFFVLIAVGASAAVN
                     LR"
     gene            3169..3504
                     /locus_tag="VF517_17115"
     CDS             3169..3504
                     /locus_tag="VF517_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054711.1"
                     /translation="MTPSLALDYLAELSTDIRTGVLLDSAGGLVSAWNGDEERGERVR
                     ELTTRLLERVDAEAGGEPPPQVEVSTGAGEVFCVRDEAWTIAVVTGRFALASLMFYDL
                     RKVLEDLSG"
     gene            3501..3725
                     /locus_tag="VF517_17120"
     CDS             3501..3725
                     /locus_tag="VF517_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054712.1"
                     /translation="MREPRNQFAKIADTITGWSNRRQRSREPRVLVYDTAGHPRLVRP
                     ESDAHDGIVEVAEQLVALTTAEPPAEAGGE"
     gene            3722..5110
                     /locus_tag="VF517_17125"
     CDS             3722..5110
                     /locus_tag="VF517_17125"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8054713.1"
                     /translation="MTLIGPDLASRAIERALRHGGDFAEVYAEESSGMSVSLDDRRIE
                     RPQTGVERGASVRVVAGDATYFGHVDGLNEDDLLRVADSVAQAARAGNAKPAGIGAPA
                     DPRGLHPVELAPGGVEAARKAELLRACDEAARAAGNEVVQVSIGYAESHRLVEVFNSD
                     GISAADDRTRVRLGVQVVARRDGTVETGAETRGGHAGFELVAERPEQIAEEAARKALT
                     LLDARDAPSGQMPVVVGNGFGGVLLHEAVGHGLEADAIQKSASIYAGRIGQRIAPPFV
                     TAYDDGSRRGEWGSDGIDDEGFPTQRTTIIEDGELTSYLYDLVRARKDGVASTGNGRR
                     ESFRHLPVPRMTNTFFAEGNATPEQLIEATDRGFYAVSFGGGQVEPATGDFVFGVSEG
                     YLIEGGKVTAPVRGATLIGNGLGALQAIDGVAADLEIATGFCGKAGQRVAAGVGQPHV
                     RIAAMTVGGTGS"
     gene            5107..6465
                     /locus_tag="VF517_17130"
     CDS             5107..6465
                     /locus_tag="VF517_17130"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8054714.1"
                     /translation="MSEPLDLEAVARRAVEAALDAGADDADAYAERSVEREIRVYDGG
                     VESLTDASSRGVGVRAFVGGRWGHAYGTDLGDDGLRQLAAAAHAAAAIADPDEHAGLP
                     EESGSADVGDLRSAELASWSTERKIELALEVERGARNNPEVSQVEQVVYADSDAEAAL
                     SGSRGFTGSFATSAAWAYASAFAGEGEDLMTGLGLGIGRGPEALDARAIGAEAGERAA
                     SLSGARQPSSRRCPVVFDAFVSASFLGFIGGMLSADAVQRGRSLFADREGDEIASPLL
                     RLADDGTAPDGLATAPFDGEGSPRRRVGLIEDGRLLTFLFDARTARKAGRATTASAVR
                     ASYRTPPSVGTGNLVVEPGEGTLEDLLRQAGDGLYVTDVVGLHSGVNPVSGTFSVGAT
                     GRLIENGELGAPVREITIASDLVSMLRAVEGVGSEARWVPFGGSVRAVPLLLREMAVS
                     GS"
     gene            6606..6932
                     /locus_tag="VF517_17135"
     CDS             6606..6932
                     /locus_tag="VF517_17135"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX8054715.1"
                     /translation="MLASPTYPATKGEQPVTKQEFVDQVASSSGLGKGEATKAVDAVL
                     DTISETLARGGEISFTGFGKFTVAERGARQGVNPQTGERIQIAASRVPRFSAGSALKN
                     AVKGGG"
     gene            6994..7920
                     /gene="pyrF"
                     /locus_tag="VF517_17140"
     CDS             6994..7920
                     /gene="pyrF"
                     /locus_tag="VF517_17140"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX8054716.1"
                     /translation="MSRGFPDRLAALVEERRSQIVLGLDPDPAALWPVALEDVPEPSE
                     DPEQIASLTAAAVASHCRAAIAAAGPACVAVKLQAACFERLGSPGWRALGESIGHARE
                     AGLLAIVDGKRGDVPVTARAYAQALVGETPGPFGPVRGLGADAFTANPLLGRDSLEPL
                     TEAADAAGAGVFCLVRTSNPGAAAIQDEPADRPLHDTLARLVDDLGAGSVGDCGLAAV
                     GAVTGATRPELLGRLRELMPRAILLLPGVGAQGGDVADLAAAFEPHPAAGLVTASRSI
                     VNAHADRGGDPAEAARAAAEDLRAVAWGLAGG"
     gene            complement(7981..8643)
                     /locus_tag="VF517_17145"
     CDS             complement(7981..8643)
                     /locus_tag="VF517_17145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054717.1"
                     /translation="MAATRTDESYLPALAYDRLTPLFDAFVRWPMREQRFKTRLVEQA
                     RVGPGQRVLDIGCGTGTLAIMVKRGQPAAEVVGLDADPTILERGRAKVAASGLDVRLD
                     QGMSFDLPYEDGSFDRVLSSLFFHHLKPGAKESTAREIARVLRPGGELHVADFGRPAG
                     PVQAAAFAAVRRFDGDDETRDNMLGRLPGFFTSAGLADARVNGALRTMMGTLEFVSAR
                     RA"
     gene            8730..13109
                     /locus_tag="VF517_17150"
     CDS             8730..13109
                     /locus_tag="VF517_17150"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8054718.1"
                     /translation="MTCGSCGYENPSDARFCAGCGRPLAARPAETDATAARSESRAAP
                     TELAGGRYVVKGFLGEGARKLVYLAHDTALDRDVAIAMLKTEGLDQTARLRLEREAQA
                     MAQLGDHPNVVAVFDIGEEDGEPYIVSQYMAGGSLDRRLSEAPGNRLTVAETFEVAES
                     VAAALEHAHSRGIVHRDLKPANVWLTAEGLAKLGDFGLAFSVERSRLTRAGSIVGTVS
                     YMAPEQALGKAPDACSDLYSLGALLYEALTGRPPFVADSAVAVISQHTSAEPVAPSWH
                     NPEVPGELEQIVLKLLEKDPDARHRDAAELRAALHDARNAAERSDGVAADHHDPVSGL
                     AEGVFVGREKEVGRLHAALDDALAGRGRLVMLVGEPGIGKTRTAQELVTYARLRGARV
                     LIGRAYEGDGAPAYWPWVQIARTYIQDRPRDELAADMGPGATDIAQVIADVRGVIPEN
                     GFAEPSAAADSEQARFRFFDSVTTFLRNASRREPLVIVLDDLHWADAPSLLLLEFMAR
                     ELGGSRLLVVGTYRDVELSRKHPLSGVLAELARESLVERVLLRGLAQPDVARFIEMTA
                     SIRPPERLVRTVHEETEGNPFFVSEVVSLLASEGHLESGADISDVLRTIPQGVREVVG
                     RRLDRLSADCNWVLAAACVVGREFAVDVLAPVVCDEATEAFPEKDPGELTRQRLLEVL
                     DEAVEARVIEAAAVGRFTFSHALVREALYEELGVTRRVRLHRRVGEMIERVYGADSEQ
                     HLDQLAHHFLEAQELEKAVDYSIRAADRAMSLMAYEDGAALYERALQALELRGGASGR
                     QRAELLIALGDAQVRAGEGQRGKETFYRAADVARAAGAHDQLARAALGVANKYEIGRV
                     ERELIELVEEALTGCGEEPSALRAELMASLAVTLYFEDYRRADELSEEAVEMARAAGD
                     SHTLAAALAARHFAVWEPERLDERLAVASELIEVAGRSGDRELKVDGHGLRIIDLLEH
                     GDIRAVDVEMEAYEREARELRQPNYLRVAAIRKAMRSVLAGRFDEAERIFEDTPSFER
                     AKRLLEPNTVQAGAVVLFELRRLQGRLDQMSNAFEQFASAYPAVVAWRAAVAVAWSEN
                     GMEDRARAEIRELARDDFAGMQRDANWLIGMVCVSLVSVRVGERAVAAAAYRLLEPYA
                     DRNVVVGGGWACEGSASLYLGYLAAHLGRYERAERHFQAALAMNERIGARPFVAETQV
                     AYAELLADRDGTAGERRAAELVDLGLQTAQEIGMAGLVERAFTLKLRLQGIEGTDVGT
                     SIDAVAAAIEDERPDLSTAAAPDGTVTIMFSDIEDSTAMTERLGDLRWMEVLREHNRI
                     VREQVAAYGGFEVKSQGDGFMVAFASASRAVECAAAVQRAFVQAAEEDPEAAIRVRIG
                     LHTGEAIRERDDFFGRNVILAARIAAQAAGGEVLVSSLLKELTESAADVRFGEPRELS
                     LKGLSGTYQVHAVEWEGLVAGAA"
     gene            13185..13553
                     /locus_tag="VF517_17155"
     CDS             13185..13553
                     /locus_tag="VF517_17155"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX8054719.1"
                     /translation="MQTTKRTTARVLAPLALVAFGLALLLIVASGGGGGDGSSNRSAT
                     DQEQRDLELAKDKRRRQRSSNRDEGQGKVSSATYVVKTGDTLGAIAEKTGVPVEKLQE
                     LNPELDPQALVSGQKIKLRE"
     gene            13550..14752
                     /locus_tag="VF517_17160"
     CDS             13550..14752
                     /locus_tag="VF517_17160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX8054720.1"
                     /translation="MTRGRWGAALAALWMLLAAAPAGAAERPVVSAPSAIVVDERTGD
                     VLFQKAPDSERSIASTTKLMTALLTLERAAPTDVFRAADYRAGAIESKIGLRPGERMR
                     VQDLLVALLLESANDAAVTLAEGVSGSREQFVADMNARAAALGLDGTSYANPIGLDDP
                     DNHSSARDLATLARLLLRNERFAEIVDRPVATLRSGARRRTLANRNRLVRTYPFVDGV
                     KTGHTHSAGYVLVGSATRGGASVVSVVLGTPSEAARDADSLTLLDWGLDRFRRVRAVR
                     AGRALRSAAVKHFDGRRVGLEAGRSVALTVRRGARVTTRVDAPDELEGPLPAGRRVGT
                     AVVLVNGKPAARVPLVTASAVPEAGFIRRSGHAIGWAVAFAVLAAAVLVTVLYRGSRS
                     RRRGGVAT"
     gene            14749..15720
                     /locus_tag="VF517_17165"
     CDS             14749..15720
                     /locus_tag="VF517_17165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEX8054721.1"
                     /translation="MIVTVTLNAALDRTLSVPNFRLGRRHRAVDSTTMAGGKGVNVAR
                     ALKTLGQPVIATGVAGGQTGTRLIEQLTGEAILNDFVRIQGESRTSTAVIDPTSGAQT
                     EINERGPSVAEAELELFVDKLLYLAKGAGVVVICGSLPAGVPVETYARLVKELKATGV
                     TTVVDSEGEPLRLATRAEPEVVTPNVLEAEELVGHEFSDYEDDLPAAAREVVDMGARE
                     AIVTHETGCVAVVSEEGERRLCRVTIDPTHVDVSSGVGSGDAFLAGYVASRYTGSPAE
                     ECLRFAVACGAESVQHFGAGVIDPREAERLMGHVSLEPLPEPALHAS"
     gene            15805..17130
                     /locus_tag="VF517_17170"
     CDS             15805..17130
                     /locus_tag="VF517_17170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX8054722.1"
                     /translation="MEIEIGRGKKGRRAYGFDDIAIVPSRRTRDPDDVDITWTLGPYR
                     FELPLLAAAMDGVVSPRTAAIVGKLGGLAVLNLEGIFTRYEDAEEQLARISKYPPEKA
                     TAEMQRIYAEPVKEELVAQRVREIKEAGVVCAASLTPQRVTKYYETALEAGLDILVIQ
                     GTVISAEHVSVTSEPLNLKEFIREVPVPVIVGGCASYSTGQHLMRTGAVGVLVGVGPG
                     AACTTRGVLGIGVPQATAIADVQAARSQHMLETGEYVNVIADGGMGTGGDISKAFACG
                     ADAVMIGSPLARAYEAPGHGFHWGMATFHPSLPRGARVRTTQNGTIEQILLGPAHEND
                     GTFNLMGSLRTSMATCGYEDIPSFHRAEVMVAPALQTEGKLLQRSQEIGMGATRAAAA
                     APTNGSTPPSADGVVEVPAQPDTPSADPAIAPAADTVATPSADPALVGE"
     gene            17127..18737
                     /gene="guaA"
                     /locus_tag="VF517_17175"
     CDS             17127..18737
                     /gene="guaA"
                     /locus_tag="VF517_17175"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX8054723.1"
                     /translation="MTGAAPATGTASEEAAAAREPAPTRSQEVLVLDFGGQYSQLIAR
                     RVRECGVFSELLPHTVEIEEIRKRAPRGLILSGGPASVYSDGAPKLPLELLELGVPVL
                     GICYGMQAMVLELGGRVEGAPVGEFGRSTLRVQEHGRLLAGLPAEQSCWMSHRDTVFE
                     PPPGFTALASSNESPVAALEDAERGLYGIQFHPEVVHTPYGTAILKTFLDDVCGCDMS
                     WNAESVAEEQIRRIREQVGDGKVICGLSGGVDSSVAALLVHRAVGDQLTCVFVDHGLM
                     RKNEGDQVVAAFRDHFKVPLVAVDAEERFLARLAGVTEPEAKRKIIGTEFIRVFEEQA
                     REIGDPRYLVQGTLYSDVIESGGGHGAATIKSHHNVGGLPEDLEFELVEPLRSLFKDE
                     VREVGLALGLPERLVWRQPFPGPGLAIRIVGDEVNKERLEILRDCDFVLQDEIRGAGL
                     YRELWQSFCVLPAGLRTVGVQGDERSYGYAIAIRAVTSDDAMTADWARLPYDLLEKIA
                     SRMINEIPAVNRVVLDITSKPPGTIEWE"
     gene            complement(18739..19128)
                     /locus_tag="VF517_17180"
     CDS             complement(18739..19128)
                     /locus_tag="VF517_17180"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8054724.1"
                     /translation="MLVVDASAALWVCGHSHGFDRLGEDLVAPHLMWSEAAAALRAWA
                     YRGAVSDEVALDHLDRVESSPIARRDHPDLRREAWRIAKGFGWKRTYDAEYVALAMLL
                     DARVVTADGRLRRGTDRLRLVIGVDEV"
     gene            complement(19587..>20130)
                     /locus_tag="VF517_17185"
     CDS             complement(19587..>20130)
                     /locus_tag="VF517_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054725.1"
                     /translation="AIYEDLEEHELLELRAEIRIHPLHQVINAARSFVKVAATYGASK
                     DEIRGVRETLQILELMAQPGPGEDSTFLVFAETTGTRDGYRLVLPVHEKHLLVPLDDF
                     AGRATFVAQVDTIIPKHDQVLAIRLIRNAPQLAMERQGLEEALPELIEAFDELGIETQ
                     HDEFFLTAPAVILKPICIYK"
BASE COUNT         2811 a         7308 c         7359 g         2652 t
ORIGIN      
        1 tcgaggcgta cgccaccgag ctgggcgggc ggctggtcga ccagggggcg aagctgctcg
       61 tcgtcgcgtg caactcggcc acggccgcgg cgctgccgtc gctgcgcgcg acgctcggcc
      121 tcgaggtgcc gatcgtcgct gtcgtcgagc ccgacgcgcg cctggcggcg caggtcaccc
      181 agaacgggca ggtcggtctc ctcgccacgg aggccaccgt cgccagccgc gcctacgacc
      241 acgcgctcgc ccagcacgcc cccgacgcca cgctgcatgc cgtcgcctgc cccgagcttg
      301 cgcccctcat ccaggcgggc gaggagatcg acgtgcgcgt gctgcggctg atcgagggct
      361 actgcgaccc gttgcgccga gcgggtgtcg acaccgtgat cctcggctgc acccactacc
      421 cgctcgttcg gccgatgctc cagcgcgagc tggggcgcga cgtgatgctc gtcagctcgg
      481 ccgaggccgt ggccgacgcc gtcgagcgcg agctcgacgc cggcgggctg tccgcgccgc
      541 tcgaccgccg cggcagctac cgcttcatgt gctcgggcga cccggacgag ttccggcgcg
      601 tcggcacgcg ctttctccag ctcccgatgg aggacgtgga gcacgtcgac gtggcggggg
      661 tcgcggcatg agcggcggca cgcgcaacga cggccgctcc gccgccgagc tgcgccccac
      721 gacgatcaca ccggacttca tgcgcacggc caccgggtcg gcgctgatcg agcagggcgg
      781 cacccgcgtg atctgcaccg cctcggccgt cgaggacgtg ccgcgctgga tgcgcgggaa
      841 gggcaccggc tgggtcaccg ccgagtacgg gatgctcccg gcctcgacgg gggaccgcaa
      901 gcagcgcgac gtctcgaagg gccgccccga cggccgcacc gtcgagatcc agcgcctgat
      961 cggccgctcg atccgcggaa tcgtcgactt cgccgccctc ggcgagcgca ccatctatct
     1021 cgactgcgac gtgctcgagg ccgacggcgg cacccgctgc gcctcgatca ccggtgccta
     1081 cgtcgccctc cacctggcct gtgcccgcat ggtcgacgag aagaagctca gcgcgattcc
     1141 gttgacgcag agtgtcgcgg ccgtctcttg cgggatggtc ggcggcatcc cgctgctcga
     1201 cctcgactat tccgaggact cgaccgcgga ggtcgacgcc aacgtggtca tgaccggcga
     1261 cggcggcctc gtcgaggtcc aggccaccgc cgagcgcacc ccgctctcgc gcgcctcgct
     1321 cgacgacctc ctcgcgctcg ccgcggccgg catcgagcgc ctgcggaggg cgcagctcga
     1381 ggccgtcggc gcgccggtcg cctgaggtag cggcgtgcag ctggtgctgg ccacccgcaa
     1441 cgagcacaag ctgcgcgagc tgcgcgagct tctgcccggc gtcgagttgc tcgcgctgcc
     1501 cgccgacgtg gtgatgccgc ccgagaccgg cgacaccttc gtggcgaacg cgatcatcaa
     1561 ggccgaggcg gccgtcgcgg ccaccggcct ccccgccgtc gccgacgact cgggcatcga
     1621 ggcttccgcc ctcggcgggg cccccggcgt gtactcggcc cgcttcgccg gcgagcacgc
     1681 gtccgaccag gagaacctcg acaagctcct gcgcgaggtg acccccgact ccgacccgcg
     1741 cctggcctac gcctgcgtgc tcgcctacct gcccgcggcc ggcgccgagc cggttctgtt
     1801 cgagggccgc tgcacgggga cgatcaccca cgacccgcgc ggcgagcacg ggttcggcta
     1861 cgacccggcc ttcgtccccg acgatcgcga cgacggcctc acgatggccg agctcgaccc
     1921 gagggacaag gacgcgatca gccaccgcgg ccgtgcggcg cgcgcgttcg ccagctggct
     1981 ggagacagcg ggcgcgcgcg cgggcgacgg cgaggccacg gaccgcggcg ggagcggcgg
     2041 cgaggccacg gaccgcggcg cggacggccg ggccacggac cgcggcgagg gcgaggcggc
     2101 gtgatcgtcg ccgtcgagaa cgccgcgcac gccgaccgca agaccagcgc ggcgcgcctg
     2161 tcgatcgcgt cgaacgccgc gctgatcgtc ctgaagctcg tggcgggcgc catcaccggc
     2221 tcgatcgcga tcatcaccga ggcggtccac tcgagcatcg acctgatggc gtcgatcgtc
     2281 gcctacttct cgatccgcaa ggccgaggag cccgccgacg agtcgcaccc ctacggccac
     2341 gagaaggtcg agaacctggc cgccgccctc gagggcgtgc tcatcctcgt gggcgcgggg
     2401 atcatcgtct tcgagtcctc gcgccggctg gtggacccgc cggaggtcga gtcgctcggc
     2461 ttcggcatcg ccgcgatcgc ggtctcggtg gtggtgaacc tcgtggtgtc gagctacctg
     2521 taccggcgcg cgaagcagac gcactcaccg gcgctcgagg gcgacgcggc ccacctgcgc
     2581 acggacgcgg tcacctccgt ggccgtcctg ctcggcctcg cgctcgtcgc gatcacggac
     2641 acgaaggcgc tcgacccgat cgtcgcgctg gcggtcgcgg ccgcgatcgt ctgggccggg
     2701 gtccggctga tcacgcgctc ctcgcgcgtc ctcgtcgacg aggccctccc cgaggacgag
     2761 ctggccgcga tcgccctggc cgtcgaaagc agcggcgccc ccgaggtggc cggcttccac
     2821 aagctccgcg ctcgccgcgc cggcagccgc cgctacatcg acctccacgt ccagttccgc
     2881 ccgggcacca cgctcgagcg cgcgcacagc gtcagccacg agctccaggc cgacatccgc
     2941 cgccgcgtcc accgcgccga cgtgctcatc cacatcgagc ccgagagctg atcagcgcag
     3001 gttcactgcg gccgacgcgc ccacggcgat cagcacgaag aagtacccca cgtagccgct
     3061 gagcagcacg aggaccgcgg agatcaggca gatccccacg gtcaccatcc acgcgattcg
     3121 ggacaggggc acggcgctat tctgccccgt caggtggccg gacccgcatt gacccccagt
     3181 ctggctctcg attacctggc ggagctgtcc accgacatcc ggaccggagt gctcctcgac
     3241 tcggccggtg gactggtctc cgcctggaac ggcgacgagg aacgcggcga acgcgtccgc
     3301 gagctcacga cccggttgct cgagcgcgtg gacgccgagg ccggcggcga gccgcccccc
     3361 caggtggagg tctcgaccgg cgccggcgag gtcttctgcg tgcgcgacga ggcctggacg
     3421 atcgcggtcg tgaccggccg cttcgcgctc gcctcgctga tgttctacga cctgcgcaag
     3481 gtcctggagg acctcagcgg atgagggagc cacgcaacca gttcgcgaag atcgcggaca
     3541 cgatcacggg ctggagcaac cgccgccagc gcagccgcga gccgcgcgtg ctcgtctacg
     3601 acaccgccgg tcacccgcgg ctggtgcggc ccgagagcga cgctcacgac ggcatcgtcg
     3661 aggtcgccga gcagctcgtg gcgctcacga ccgcggaacc ccccgcggag gcgggcggcg
     3721 aatgacgctg atcgggcccg acctcgcctc acgcgcgatc gagcgcgccc tgcgccacgg
     3781 cggcgacttc gcggaggtct acgccgagga gagcagcggg atgtcggtat cgctcgacga
     3841 ccggcggatc gagcgccccc agacgggggt cgagcgcggg gcgtccgtgc gcgtggtggc
     3901 cggcgacgcc acctacttcg gccacgtgga cgggttgaac gaagacgacc tgctgcgcgt
     3961 ggcggactcg gtcgcccagg ccgcgcgcgc ggggaacgcc aagccggccg ggatcggcgc
     4021 gccggcggac ccgcgcggcc tgcacccggt cgagctggcg ccgggcggtg tcgaggccgc
     4081 ccgcaaggcc gagctcctgc gcgcctgcga cgaggccgcc cgtgccgccg ggaacgaggt
     4141 ggtccaggtc tcgatcggct acgcggagag ccaccggctg gtggaggtct tcaactccga
     4201 cgggatctcc gcggccgacg accgcacacg cgtgcggctc ggcgtgcagg tggtcgctcg
     4261 ccgcgacggc accgtcgaga ccggcgccga gacccgcggc gggcacgccg gcttcgagct
     4321 ggtggccgag cggcccgagc agatcgccga ggaggccgcc cgcaaggccc tcacgctgct
     4381 cgacgcgcgc gacgcgccga gcggccagat gcccgtcgtg gtcggcaacg gcttcggcgg
     4441 cgtgcttctg cacgaggccg tcgggcacgg cctcgaggcc gacgcgatcc agaagagcgc
     4501 gagcatctac gcgggcagga tcggccagcg catcgcgccg ccgttcgtca ccgcctacga
     4561 cgacggctcg cgccgtggcg agtgggggag cgacggcatc gacgacgagg gcttcccgac
     4621 ccagcgcacc acgatcatcg aggacggcga gctcacctcg tacctgtacg acctcgtgcg
     4681 cgcgcgcaag gacggcgtcg cctccaccgg caacgggcgg cgcgagtcgt tccgccacct
     4741 gccggtgccg cgcatgacca acacgttctt cgccgagggc aacgccaccc ccgagcagct
     4801 gatcgaggcc accgaccggg ggttctatgc ggtgtcgttc ggtggcggcc aggtcgagcc
     4861 ggcgaccggg gacttcgtgt tcggcgtctc ggagggctac ctgatcgaag gcggcaaggt
     4921 caccgcgccc gtgcgcggcg ccacgctgat cggcaacggc ctcggcgcgc tccaggcgat
     4981 cgacggcgtg gcggcggacc tcgagatcgc caccggcttc tgcggcaagg cgggccagcg
     5041 ggtggccgca ggcgtcggcc agccacacgt gcgcatcgcg gcgatgacgg tcggcgggac
     5101 cgggtcgtga gcgagccact cgacctggag gcggtcgcgc gccgcgcggt cgaggccgct
     5161 ctcgacgcgg gcgccgacga cgccgacgcg tacgccgagc gctccgtcga gcgcgagatc
     5221 cgcgtctacg acggcggcgt cgagagcctg accgacgcct cgagccgcgg ggtcggcgtc
     5281 cgagcgttcg tcggcggccg ctggggtcac gcctacggca ccgacctcgg cgacgacggc
     5341 ctgcgccagc tcgccgccgc cgcgcacgca gcggcggcca tcgccgaccc cgacgagcac
     5401 gccggcctcc ccgaggagtc cggctcggcc gacgtgggcg acctccgctc ggccgagctc
     5461 gcgagctgga gcaccgagcg caagatcgag ctggcactcg aggtcgagcg gggcgcgcgg
     5521 aacaacccag aggtgtcgca ggtcgagcag gtcgtctacg ccgacagcga cgcggaggcg
     5581 gcgctgtcgg gctcgcgcgg cttcaccggc tcgttcgcca cctccgcggc ctgggcctac
     5641 gcgagcgcgt tcgcgggcga gggcgaggac ctgatgaccg ggctcggcct cggcatcggc
     5701 cgcgggccgg aggcgctcga cgcgcgcgcg atcggcgccg aggccggcga gcgcgcggcg
     5761 tcgctgtccg gcgcgcgcca gccgtcgagc cggcgctgcc cggtcgtctt cgacgccttc
     5821 gtgtcggcca gcttcctcgg cttcatcggc ggcatgctct cggccgacgc agtccagcgc
     5881 gggcgctcgc tgttcgccga ccgcgagggc gacgagatcg ccagcccgct gctgcgcctg
     5941 gccgacgacg gcaccgcgcc cgacggcctc gccacggcgc cgttcgacgg cgagggctcg
     6001 ccgcgccgcc gcgtcgggct gatcgaggac gggcggctgc tcacgttcct gttcgacgcg
     6061 cgcaccgcgc gcaaggcggg ccgggccacc accgcgagcg cggtacgcgc gtcctaccgg
     6121 acgccgccct cggtgggcac gggaaacctg gtcgtcgagc cgggcgaggg gaccctcgag
     6181 gacctcctgc gccaggccgg cgacggcctc tacgtgaccg acgtggtcgg cctgcactcg
     6241 ggggtgaacc cggtgtcggg caccttttcg gtgggcgcga ccggccgcct gatcgagaac
     6301 ggcgagctgg gcgctccggt gcgcgagatc acgatcgcga gcgacctcgt gtcgatgctg
     6361 cgggcggtcg agggcgtcgg ctcggaggcc cgctgggtcc cgttcggggg cagcgtccga
     6421 gccgtcccgc tgctgctgcg ggagatggcg gtctccggct cttagagccg tgagcaaagg
     6481 ccgcgcgggc ggcccgcggg cgccgcgccg cccgtttcac gcccgtttca cacgccgaaa
     6541 aacccgcttc ctcggcaaaa cccgcattcc agagcggaat cgggcgcgga agtcgtccgt
     6601 gaccgctgtt agcttcgccg acgtacccag ccacgaaggg ggaacaaccc gtgaccaagc
     6661 aggaattcgt cgatcaggtg gccagctcgt ccggcctcgg caagggcgag gccaccaagg
     6721 ccgtagatgc cgttctcgac acgatctcgg agacgctcgc ccgcggcggg gagatcagct
     6781 tcaccggctt cggcaagttc accgtcgccg agcgtggggc ccgtcaggga gtgaacccgc
     6841 agaccggcga gcggatccag atcgccgcgt cgcgcgtccc gcgcttcagc gccggctcgg
     6901 cgctcaagaa cgcggtcaag ggcggcggat agcccaccgg gccgtccgcg gccggggagc
     6961 cgcggcccag gcgtcccctg cgaggggcga cgcgtgagcc gcggctttcc cgatcgcctc
     7021 gcggcgctcg tcgaggagcg ccgcagtcag atcgtcctcg gactcgaccc ggacccggcc
     7081 gcgctgtggc cggtcgcgct cgaggacgtc ccggagccgt ccgaggatcc ggagcagatc
     7141 gcgtcgctca ccgccgcggc ggtcgcgagc cactgccgcg ccgccatcgc ggcggcgggc
     7201 cccgcctgcg tcgccgtgaa gctccaggcc gcctgcttcg agcggctcgg ctcgcccggc
     7261 tggcgggcgc tcggcgagtc gatcgggcac gcccgcgagg cgggcctgct cgcgatcgtg
     7321 gacggcaagc gcggcgacgt gccggtgacc gcgcgcgcct acgcgcaggc gctggtgggc
     7381 gagaccccgg ggccgttcgg ccccgtccgc gggctcggcg ccgatgcctt caccgcgaac
     7441 ccattgctcg ggcgcgactc gctcgagccg ctcaccgagg ccgccgacgc ggccggcgcg
     7501 ggcgtcttct gcctcgtgcg cacctcgaac cccggcgcgg cggcgatcca ggacgagccg
     7561 gccgaccggc ccctgcacga cacgctggcc cgcctggtcg acgacctcgg cgcgggcagc
     7621 gtcggcgact gcgggctcgc tgcggttggg gccgtgaccg gcgcgaccag gccggagctg
     7681 ctcggccgcc tgcgcgagct gatgccgcgc gcgatcctgc tgctgccggg cgtgggggcg
     7741 cagggcggcg atgtggcgga cctggcggcg gcgttcgagc cccacccggc ggccgggctc
     7801 gtcaccgcct cgcgctcgat cgtgaacgcg cacgccgacc gcggcggcga tccggccgag
     7861 gccgcgcgcg cggcggcgga ggacctccgc gccgtggcgt gggggctcgc gggcggctag
     7921 gcccgcgccc cgccgcggct agccccgcgc tcccgcgggg cccgcggcta cgccgcgccg
     7981 ctacgcccgg cgcgccgaga cgaactcgag cgtgcccatc atcgtgcgga gcgcgccgtt
     8041 cacgcgcgcg tccgccagac ccgccgaggt gaagaacccg ggcaggcgcc cgagcatgtt
     8101 gtcgcgcgtc tcgtcgtcgc cgtcgaagcg ccgcacggcg gcgaacgccg ccgcctggac
     8161 cggcccggcc ggcctcccga agtccgccac gtgcagctcg ccccccggcc tcagcacccg
     8221 cgcgatctca cgcgcggtgc tctccttcgc gccgggcttc aggtggtgga agaacaggct
     8281 cgacagcacg cggtcgaacg acccgtcctc gtagggcagg tcgaacgaca tgccctggtc
     8341 gagccgcacg tcgagtccgc tcgcagccac cttcgcgcgc ccgcgctcga ggatcgtggg
     8401 atctgcgtcg agcccgacca cctcggccgc cggctggccg cgcttgacca tgatcgcgag
     8461 cgtgccggtg ccgcacccga tgtcgagcac ccgctgcccc ggtcccaccc gggcctgctc
     8521 gacgagccgc gtcttgaagc gctgctcgcg catcggccag cgcacgaacg cgtcgaacag
     8581 cggcgtgaga cggtcgtacg cgagggcggg aaggtaggac tcgtctgtgc gcgtcgccgc
     8641 cacgcggccg aacgctactc ctcgtccgcg cacgcggggt gtgacatagc cctctttccc
     8701 acagaggcgc cgcctgcgaa gatcgtcaca tgacctgcgg gtcatgcggc tacgagaacc
     8761 cgtccgacgc tcgcttctgc gccggttgcg gacggccgct cgcagcccgg cccgccgaga
     8821 ccgacgcgac cgccgcccgg agcgagtccc gcgcggcgcc gacggagctg gccggcggcc
     8881 gctacgtcgt caagggcttc ctgggcgagg gcgcccgcaa gctcgtctac ctcgcccacg
     8941 acaccgccct cgaccgcgac gtggcgatcg cgatgctgaa gaccgagggc ctcgaccaga
     9001 ccgcccgcct gcggctcgag cgcgaggcgc aggcgatggc gcagctcggc gaccacccca
     9061 acgtcgtcgc ggtcttcgac atcggcgagg aggacggcga gccctacatc gtcagccagt
     9121 acatggccgg cggctcgctc gaccggcggc tctccgaggc gcccggcaac cggctcacgg
     9181 tcgcggagac gttcgaggtc gccgagtcgg tggccgccgc gctcgagcac gcgcacagcc
     9241 gcggcatcgt gcaccgcgac ctcaagcccg cgaacgtctg gctcaccgcc gagggcctgg
     9301 cgaagctggg cgacttcggc ctcgcgttct cggtcgagcg ctcgcgcctc acgcgcgccg
     9361 gatccatcgt gggcacggtc tcctacatgg cccccgagca ggcgctcggc aaggcgccgg
     9421 acgcatgcag cgacctctac tcgctcggcg cgctgctcta cgaggccctc accgggcgcc
     9481 cgccgttcgt cgccgacagc gccgtcgccg tgatctcgca gcacacgagc gcggagccgg
     9541 tggcgccgag ctggcacaac ccggaggtgc cgggcgagct cgagcagatc gtcctcaagc
     9601 tcctcgagaa ggatccggat gcgcgccaca gggacgcggc cgagctgcgc gccgccctgc
     9661 acgacgcccg caacgccgcc gagcgctcgg acggcgtcgc ggccgaccac cacgaccccg
     9721 tcagcggcct ggccgagggc gtcttcgtcg gccgcgagaa ggaggtgggc cgcctgcacg
     9781 ccgcgctcga cgacgcgctc gccggccgcg gccgcctcgt catgctcgtc ggcgagcccg
     9841 gcatcggcaa gacgcgcacg gcgcaggagc tcgtcaccta cgcgcgcctg cgtggcgcgc
     9901 gggtgctgat cggccgcgcc tacgagggcg acggcgcccc ggcgtactgg ccgtgggtcc
     9961 agatcgctcg cacctacatc caggaccgcc cgcgcgacga gctcgccgcc gacatgggcc
    10021 cgggcgcgac ggacatcgcc caggtgatcg ccgacgtgag gggcgtgatc cccgagaacg
    10081 gcttcgccga gccctcggcg gccgccgact ccgagcaggc gcgcttccgc ttcttcgaca
    10141 gcgtgaccac gttcctgcgc aacgcctcgc gccgtgagcc gctcgtgatc gtgctcgacg
    10201 accttcactg ggccgacgcg ccgtcgctcc tgctgctcga gttcatggcg cgagagctgg
    10261 gcgggtcgcg gctgctcgtg gtcggcacct accgggacgt ggagctgagc cgcaagcacc
    10321 cgctctccgg cgtcctcgcc gagctggcgc gcgagagcct cgtggagcgc gtcctgctgc
    10381 gcgggctcgc acagccggac gtcgcccgct tcatcgagat gaccgccagc atccggccgc
    10441 cggagcgcct ggtgcgaacg gtgcacgagg agaccgaggg caacccgttc ttcgtgtcgg
    10501 aggtggtgag cctgctcgcg tccgagggcc acctcgagag cggcgcggac atctcggacg
    10561 tgctgaggac gatcccccag ggcgtgcgcg aggtcgtggg ccgccggctc gaccggctct
    10621 ccgccgactg caactgggtg ctggcggcgg cgtgcgtggt cggccgcgag ttcgcggtgg
    10681 acgtgctcgc gccggtcgtc tgcgacgagg ccaccgaggc gttcccggag aaggacccgg
    10741 gcgagctgac ccgccagcgg ctgctcgagg tgctcgacga ggcggtcgag gcccgcgtga
    10801 tcgaggcggc cgcggtcggg cgcttcacgt tttcgcacgc cctcgtgcgc gaggcgctct
    10861 acgaggagct cggcgtgacc cgccgggtcc gcctgcaccg ccgcgtcggc gagatgatcg
    10921 agcgggtcta cggcgctgac tccgagcagc acctcgacca gctcgcgcac cacttcctcg
    10981 aggcgcagga gctcgagaag gccgtcgact actcgatccg cgccgccgac cgggcgatgt
    11041 cgctgatggc ctacgaggac ggcgccgcgc tgtacgagcg cgcgctccag gccctcgagc
    11101 tgcgcggcgg cgcctcgggc cgccagcggg cggagctgct gatcgcgctc ggcgacgccc
    11161 aggtgcgcgc cggcgagggc cagcgcggca aggagacctt ctaccgcgcg gccgacgtgg
    11221 cccgcgcggc cggggcgcac gaccagctcg cgcgggctgc gctcggggtc gcgaacaagt
    11281 acgagatcgg gcgcgtcgag cgcgagctga tcgagctggt cgaggaggcg ctgaccggct
    11341 gcggcgagga gccgagcgcg ctgcgcgcgg agctgatggc gagcctcgcc gtgacgctct
    11401 acttcgagga ctacaggcgc gcggacgagc tgagcgagga agcggtggag atggcccgtg
    11461 ccgccggcga ctcgcacacg ctcgcggccg cgctcgccgc gcggcacttc gcggtctggg
    11521 agccggagcg cctcgacgag cggctggcgg tggcgagcga gctgatcgag gtggcggggc
    11581 gctcgggcga ccgtgagctg aaggtcgacg gccacggcct gcggatcatc gacctgctcg
    11641 agcacggcga catccgggcc gtcgacgtcg agatggaggc ctacgagcgc gaggcgcgcg
    11701 agctgcgcca gcccaactac ctgcgcgtgg cggcgatccg caaggcgatg cgctcggtgc
    11761 tggcggggcg cttcgacgag gcggagcgca tcttcgaaga cacgccctcg ttcgagcgcg
    11821 ccaagcggct gctcgagccg aacacggtcc aggccggggc ggtcgtgctg ttcgagctgc
    11881 gccggctcca gggccgcctc gaccagatga gcaacgcgtt cgagcagttc gcgagcgcct
    11941 atccggcggt ggtcgcgtgg cgggcggccg tggcggtcgc ctggagcgag aacggcatgg
    12001 aggaccgcgc gcgggccgag atccgggagc tggcgcgcga cgacttcgcc gggatgcagc
    12061 gcgacgcgaa ctggctgatc ggcatggtct gcgtctcact cgtctccgtc cgcgtcggcg
    12121 agcgcgcggt ggccgccgcc gcctaccggc tgctcgagcc ctacgcggac cgcaacgtcg
    12181 tcgtcggcgg cggctgggcc tgcgagggct cggcgtcgct ctacctcgga tacctcgccg
    12241 cgcacctggg ccgctacgag cgcgccgagc gccacttcca ggccgccctc gcgatgaacg
    12301 agcgcatcgg cgcgcggccg ttcgtcgccg agacccaggt cgcgtacgcc gagctgctcg
    12361 ccgaccgcga cggcaccgcc ggcgagcgcc gcgccgccga gctggtggac ctcggcctcc
    12421 agaccgcgca ggagatcggg atggcggggc tcgtcgagcg cgcgttcacg ctgaagctcc
    12481 gcctccaggg catcgagggg accgacgtcg gcacctcgat cgacgcggtc gccgccgcca
    12541 tcgaggacga gcgcccggac ctgagcacgg cggccgcgcc cgacggcacc gtcacgatca
    12601 tgttcagcga catcgaggac tcgaccgcga tgaccgagcg cctcggcgac ctccgctgga
    12661 tggaggtgct gcgcgagcac aaccggatcg tgcgcgagca ggtggcggcg tacggcggct
    12721 tcgaggtgaa gtcgcagggc gacggtttca tggtcgcgtt cgccagcgcg agccgggcgg
    12781 tggagtgcgc cgcggccgtc cagcgcgcct tcgtgcaggc cgccgaggag gaccccgagg
    12841 cggcgatccg ggtgcggatc ggcctccaca ccggcgaggc gatccgcgag cgcgacgact
    12901 tcttcggccg caacgtgatc ctcgccgccc gcatcgccgc gcaggccgcc ggcggcgagg
    12961 tgctggtctc ctcgctgctc aaggagctga ccgagtccgc ggccgacgtg cgcttcggcg
    13021 agccgcgcga gctgagcctc aaggggctct ccggcaccta tcaggtgcac gcggtcgagt
    13081 gggaggggct cgtggcgggc gcggcctagc cgcacgccga ccgcacgcaa tccccgctgc
    13141 acacgcaggg aaatcgcctc cgcgcgggta tcctcaggac ggcgatgcag acgaccaagc
    13201 gcaccactgc gcgcgttctc gcgccgctcg cgctggtggc gttcggcctc gcgctgctgc
    13261 tgatcgtagc cagcggcggg ggcggcggcg acggctcctc gaaccgctcg gccaccgacc
    13321 aggagcagcg cgacctcgag ctggccaagg acaagcgcag gcgccagagg tcctcgaacc
    13381 gcgacgaggg ccagggcaag gtgtcctcgg ccacctacgt cgtgaagacg ggcgacacgc
    13441 tcggcgcgat cgccgagaag accggcgttc cggtggagaa gctccaggag ctgaacccgg
    13501 agctcgaccc gcaggcgctc gtgtccggac agaagatcaa gctccgcgag tgacccgcgg
    13561 gaggtggggg gccgctctcg cggccctgtg gatgctgctc gccgccgcgc cggcgggggc
    13621 tgccgagcgg ccggtcgtga gcgcgccgtc ggcgatcgtc gtggacgagc ggaccggcga
    13681 cgtgctgttc cagaaggcac cggactccga gcgctcgatc gccagcacca ccaagctgat
    13741 gaccgcgctg ctcacgctcg agcgcgcggc gcccaccgac gtgttccgcg cggccgatta
    13801 ccgcgccggc gcgatcgagt cgaagatcgg cctgcggccg ggcgagcgga tgcgcgtgca
    13861 ggacctgctc gtcgcgctgc tgctcgagag cgccaacgac gccgcggtga cgctggccga
    13921 gggcgtgtcg ggctcgcgcg agcagttcgt cgcggacatg aacgcgcgcg cggcggcgct
    13981 ggggctcgac ggcacgagct acgccaaccc gatcgggctc gacgaccccg acaaccactc
    14041 gagcgcgcgc gacctcgcca cgctcgcgcg gctgctcctg cgcaacgagc gcttcgcgga
    14101 gatcgtcgac cgcccggtcg cgaccctgcg cagcggcgcg cggcggcgca cgctcgccaa
    14161 ccgcaaccgg ctcgtgcgca cgtatccgtt cgtggacggc gtgaagaccg gccacacgca
    14221 ctcggcgggc tacgtgctcg tcggctcggc cacgcgcggc ggggcgagcg tcgtcagcgt
    14281 cgtgctcggc acgccgagcg aggcggcgcg cgacgccgac agcctgacgc tgctcgactg
    14341 ggggctcgac cgcttccggc gcgtgcgtgc ggtccgcgcc gggcgggcgc tgcggagcgc
    14401 cgccgtcaag cacttcgacg gccggcgcgt ggggctcgag gcgggccgct cggtcgcgct
    14461 gacggtccgc cgcggcgcgc gcgtgaccac gcgggtggac gctccggacg agctcgaggg
    14521 gccgctgccg gccgggcggc gcgtgggcac ggcggtcgtg ctcgtgaacg gcaagcccgc
    14581 ggcccgcgtc ccgctcgtca ccgcctccgc ggtccccgag gccggcttta tccgccgttc
    14641 aggtcacgcc ataggttggg cggtagcctt tgcggtgctc gcggcggcgg tgctcgtcac
    14701 ggtgctctac agagggtctc gatcgcgtcg acgtgggggg gttgcaacat gatcgtcacc
    14761 gtcacgctca acgccgctct cgacaggacc ctgtcggtgc cgaacttccg ccttggccgt
    14821 cgccaccggg ccgtggacag caccacgatg gccggcggga agggcgtcaa cgtcgcgcgg
    14881 gcgctgaaga cgctcggcca gcccgtgatc gccacggggg tcgcgggcgg ccagaccggc
    14941 acgcgcctga tcgagcagct caccggggag gcgatcctca acgacttcgt gcggatccag
    15001 ggcgagtcgc gcacctcgac cgccgtgatc gaccccacct ccggggccca gaccgagatc
    15061 aacgagcgcg ggccgtcggt ggccgaggcc gagctcgagc tgttcgtcga caagctcctg
    15121 tacctggcca agggcgccgg ggtcgtcgtg atctgcgggt cgctgccggc gggggtgccg
    15181 gtcgagacct acgcacggct cgtgaaggag ctgaaggcga ccggcgtgac gaccgtggtc
    15241 gactccgagg gcgagccgct gcggctggcc acgcgggccg agcccgaggt ggtcacgccg
    15301 aacgtgctcg aggccgagga gctggtgggg cacgagttct ccgactacga ggacgacctc
    15361 ccggccgccg cgcgcgaggt ggtcgacatg ggcgcgcgcg aggcgatcgt cacgcacgag
    15421 acgggctgcg tcgcggtggt ctccgaggag ggtgagcgca ggctctgccg cgtcacgatc
    15481 gaccccacgc acgtggacgt gagctcgggg gtcgggtcgg gcgacgcgtt cctcgccggc
    15541 tacgtggcct cgcgctacac gggcagcccc gccgaggagt gcctgcgctt cgcggtcgcc
    15601 tgcggagccg agtccgtgca gcacttcggg gcgggcgtga tcgacccgcg cgaggccgag
    15661 cggctgatgg ggcacgtgag cctcgagccg ctgcccgagc cggcgctgca cgcgtcgtaa
    15721 aacctccgca aagagcggcc aggaggccct gagggccgct gctaacgtcc tctttcagtc
    15781 cgccgccgaa cctaggatgc cgccatggag attgagatcg ggcgcgggaa gaagggccgc
    15841 cgcgcgtatg gcttcgatga catcgcgata gtgccctcgc gcagaacgcg cgacccggac
    15901 gacgtcgata tcacctggac gctcgggccc taccgcttcg agcttcccct gctggccgct
    15961 gccatggacg gggtcgtctc gccgcgcacg gccgcgatcg tcggcaagct cggcgggctc
    16021 gccgtgctca acctcgaggg gatcttcacc cgctacgagg acgccgagga gcagctcgcg
    16081 cggatctcga agtacccgcc ggagaaggcc accgccgaga tgcagcggat ctacgccgag
    16141 ccggtgaagg aggagctcgt cgcccagcgc gtgcgcgaga tcaaggaggc cggcgtcgtg
    16201 tgcgcggcct cgctcacgcc gcagcgggtc acgaagtact acgagaccgc gctcgaggcg
    16261 gggctcgaca tcctcgtgat ccagggcacg gtcatctcgg ccgagcacgt gtcggtcacc
    16321 tcggagccgc tcaacctcaa ggagttcatc cgcgaggtgc cggtgccggt gatcgtcggc
    16381 ggctgcgcct cgtactcgac cgggcagcac ctgatgcgca cgggcgcggt cggcgtgctg
    16441 gtgggcgtcg ggccgggcgc cgcgtgcacc acgcgcggcg tgctcgggat cggcgtgccg
    16501 caggccaccg cgatcgcgga cgtgcaggcc gcgcgctcgc agcacatgct cgagacgggc
    16561 gaatacgtga acgtgatcgc cgacggcggc atgggcaccg gcggcgacat ctcgaaggcg
    16621 ttcgcctgcg gggccgacgc cgtgatgatc ggctctccgc tcgcgcgcgc ctacgaggcg
    16681 cccgggcacg ggttccactg gggcatggcc acgttccatc cgtcgcttcc gcgcggggcg
    16741 cgcgtgcgca ccacccagaa cggcacgatc gagcagatcc tgctcggccc ggctcacgag
    16801 aacgacggca cgttcaacct gatggggagc ctgcgcacgt cgatggccac ctgcgggtac
    16861 gaggacatcc cctcgttcca ccgcgcggag gtcatggtcg cgccggcact ccagaccgag
    16921 ggcaagctgc tccagcgctc gcaggagatc gggatggggg ccacgcgggc agccgcggcc
    16981 gcgcccacca acgggtcgac gccgccgtcg gccgacggag tcgtcgaggt gccggcgcag
    17041 cccgacaccc cgtccgccga cccggccatc gcgccggccg cggacaccgt cgccacgccg
    17101 tcggccgacc cagctctcgt aggggagtga cgggggccgc gcccgcgacg gggaccgcct
    17161 ccgaggaggc ggccgccgcg cgcgagcccg ctcccacgcg cagccaggag gtcctggtcc
    17221 tcgacttcgg gggccagtac tcgcagctga tcgcgcgccg cgtgcgcgag tgcggggtgt
    17281 tcagcgagct gctgccgcac acggtcgaga tcgaggagat ccgcaagcgc gcgccgcgcg
    17341 ggctgatcct ctccggcggc cccgcctcgg tctactcgga cggcgcgccg aagctgccac
    17401 tcgagctgct cgagctcggg gtccctgtgc tgggcatctg ttacggcatg caggcgatgg
    17461 tgctcgagct gggcggtcgc gtcgagggcg cgcccgtcgg cgagttcggg cgctccacgc
    17521 tgcgggtgca ggagcacggc cggctgctcg ccgggctgcc ggccgagcag agctgctgga
    17581 tgagccaccg cgacacggtg ttcgagccgc cgcccgggtt caccgcgctg gcgtcctcga
    17641 acgagtcccc ggtggccgcc ctggaggacg ccgagcgcgg gctctatggc atccagttcc
    17701 acccggaggt cgtgcacacg ccctacggca cggcgatcct gaagacgttc ctcgacgacg
    17761 tctgcggctg cgacatgagc tggaacgccg agtcggtggc ggaggagcag atcaggcgga
    17821 tccgcgagca ggtgggcgac ggcaaggtga tctgcgggct ctcgggcggg gtcgactcct
    17881 cggtggcggc gctgctcgtc caccgcgcgg tgggcgacca gctcacgtgc gtgttcgtcg
    17941 accacgggct gatgcgcaag aacgagggcg accaggtggt ggcagccttc cgcgaccact
    18001 tcaaggtgcc gctcgtggcg gtggacgccg aggagcgctt cctcgcgcgc ctagccgggg
    18061 tcaccgagcc ggaggccaag cgcaagatca tcggcaccga gttcatccgg gtcttcgagg
    18121 agcaggctcg cgagatcggc gacccgcgct acctggtcca gggaacgctc tactcggacg
    18181 tgatcgagtc gggcggcggc cacggagcgg ccacgatcaa gtcgcaccac aacgtgggcg
    18241 ggctcccgga ggacctcgag ttcgagctgg tcgagccgct gcggtcgctg ttcaaggacg
    18301 aggtgcgcga ggtcgggctg gcgttgggcc tgccggagcg gctcgtgtgg cgccagccgt
    18361 tcccggggcc gggactggcg atccgcatcg tcggcgacga ggtcaacaag gagcgcctcg
    18421 agattctgcg cgactgcgac ttcgtgctcc aggacgagat ccgcggcgcc gggctctacc
    18481 gcgagctgtg gcagtcgttc tgcgtgctgc cggccgggct gcggacggtg ggcgtgcagg
    18541 gcgacgaacg ctcgtacggc tacgcgatcg cgatccgggc ggtcacgagc gacgacgcga
    18601 tgacggccga ctgggcccgc ctgccctacg acctgctcga gaagatcgcc tcgcggatga
    18661 tcaacgagat ccccgcagtg aaccgggtgg tgctggacat cacgtccaag ccgccgggca
    18721 cgatcgagtg ggagtgactc agacttcgtc gacgccaatc acaaggcgca ggcgatccgt
    18781 gccccgacgc agacggccat ccgcggttac aacccttgcg tcgagcagca tcgcaagcgc
    18841 gacgtactct gcgtcatacg ttcgtttcca gccgaagccc ttggcgatcc tccatgcttc
    18901 tcggcgcaga tcaggatgat ccctgcgagc aatcggactc gattcgacgc gatctagatg
    18961 gtcgagagcg acctcgtctg agacggcgcc gcggtatgcc caagcgcgaa gtgcggccgc
    19021 ggcttccgac cacatgaggt gcggggcaac caagtcctca ccgagccgat cgaagccgtg
    19081 cgagtgacca caaacccaga gagccgcgct ggcgtcgaca acaagcaact aatgcgattc
    19141 gcgctcagct cggatgactt ccgcccagtt tggcgctggc gttccgtcgc tgaaggtccc
    19201 ccagcctccc ggtgggtgga ggtcccgtgc acgccgaccc gctgctctac ggacatcagc
    19261 gcgatgaacc aatgtccgcc ttggaacacg gaccgcgacc aactcgccgg ccttgatcca
    19321 gcgccggaca gtgctcggcg agcggtcgac gagtactgcg gcatcggtga tcgataggaa
    19381 tccaagccgc tcaggcgaga tcgttacggg cggcgccaat ttcttaattg tcatggcttg
    19441 agacgcgcgg agcttgcgtt cggccccacc tcgtcgggac ggtacgggcg gcgccgcaag
    19501 ccatttactc ctccgaactc tctcgctgaa ggcgacgagg tccggagatg tcgacccgcc
    19561 atcccgtgtg ctcgctggag cgctcatcac ttgtaaatgc agattggctt gagaattact
    19621 gcgggggccg tcaggaagaa ctcgtcatgc tgggtttcga ttccgagctc atcgaatgcc
    19681 tcaatcagtt ccggaagcgc ctcctctaag ccctgtcgct ccatcgccag ttgtggtgcg
    19741 ttcctgatga gccggatcgc gagaacctgg tcatgcttcg ggatgatggt gtctacctga
    19801 gccacaaagg tggccctacc ggcaaagtcg tcgagaggca caagcaagtg cttctcgtgc
    19861 acggggagca caagccgata tccatctcgc gtgcccgtgg tctcggcaaa aactaggaac
    19921 gtgctgtcct cgccagggcc aggctgcgcc atgagttcaa ggatctggag cgtttctcgc
    19981 accccccgta tctcatcctt cgaggcgcca taagtagcgg cgaccttcac gaagctccgg
    20041 gccgcgttga ttacttggtg gagtggatga atccgaatct ctgcccgaag ttcgagcagc
    20101 tcgtgctctt caagatcttc gtaaatggcg
//
LOCUS       DASVPL010000302        39482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_309854, whole genome shotgun sequence.
ACCESSION   DASVPL010000302 DASVPL010000000
VERSION     DASVPL010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 39482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39482
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309854"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..546)
                     /locus_tag="VF517_17190"
     CDS             complement(94..546)
                     /locus_tag="VF517_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054726.1"
                     /translation="MRDCLAGYETELTRETCLSVWREWVAETIAPDAEFVAIRERTGH
                     NYHSWKSVFWKFIEEGRAPGWSAGEDRWLDREGTLFGRRVDGRHKLYGGSSVVVLLAG
                     ASLRPRRVQGRWLSDGHTVDERRERGGDLRAAMLACVDRATEAVDHAT"
     gene            1204..1617
                     /locus_tag="VF517_17195"
     CDS             1204..1617
                     /locus_tag="VF517_17195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX8054727.1"
                     /translation="MTESSTIGRDTAIAEVRAVFSEIYAAWADNDAEAFAAGYREDAT
                     VVMPGVRHIGKSAIRDSMAAAFQGPLKGSKAVDEPVDFRVVGDDTAIVISKAGILMAG
                     ETEVPAEREATATWVLTKQDGEWLIAAYANAPAVT"
     gene            1653..1991
                     /locus_tag="VF517_17200"
     CDS             1653..1991
                     /locus_tag="VF517_17200"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8054728.1"
                     /translation="MKYALLIHEGVGAYEQLSADELAAVTAQYVEIVQDRRVLSAEQL
                     EPPTTATTVRVRDGELLLTDGPFADTKEIFGGYYIVEARDLDDALELAGRMPAARLGG
                     SVEVRPVVER"
     gene            1997..3214
                     /locus_tag="VF517_17205"
     CDS             1997..3214
                     /locus_tag="VF517_17205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8054729.1"
                     /translation="MERVFRDEWGRVLASLVGYLGDFELAEDAAQEAFAIAAARWAHD
                     GPPDNPGGWLVMTARNRAIDRLRREHTLARKTCQLELPEPVELTLDDPMIPDERLELI
                     FMCCHPALATEVQVALTLRALGGLTTPEIARAFVVSDETMKRRLSRARTKIKAAGIPF
                     EVPADRVLPDRLRTILAVVYLIFNQGYGGGRVDLADEAIRLGRVLVDLTPDEPEAAAL
                     LALMLLHDARRDARFRDGEIVVLGDQDRTLWNAEQIIAGRAVLDRAIALRGRGPYTVQ
                     AAIASLQVEPAIDWPAVVALYGRLGELTGSPIVALNRAVAVAEVEGPHAALAIVDALE
                     IPDDYRYLHSTRGELLRRLGDHGAARDAYQRALAFTVTDPERRFLQRRLRDLSAGQPL
                     RGGGDEFRPNSGS"
     gene            3288..4025
                     /locus_tag="VF517_17210"
     CDS             3288..4025
                     /locus_tag="VF517_17210"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX8054730.1"
                     /translation="MSEHKTGTREEWQAARDDLAKLEAEHAERNEEIKRKRRELPWVA
                     VEKEYEFDTEDGKKKLADLFDGRSQLLAYNIMYGPDYELGACPGCTSLADGFDGTLVH
                     LNHADVTFVGFSRAPIDRLVAYKERMGWEFPYVSTYGSDFAFDFGLALSEEQARQIPE
                     VQQMVDDPPEWLQFWSTQVGADLKDGLREAPGYIAFALENGTVYHTYTVTAPDPFVSP
                     FFGFMLDRTPKPQPEEPRTFRKDEYPD"
     gene            4038..4766
                     /locus_tag="VF517_17215"
     CDS             4038..4766
                     /locus_tag="VF517_17215"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX8054731.1"
                     /translation="MPEHRVETQEKWQAERDALLAREKELTKLSDELAERRRELPWVA
                     VEEDYSFETTEGTKSLAELFDGRSQLLVYHFMFGPPYESGCPVCSSIADNLDPNAVHL
                     AARDTTLVLSSRAPLEKLQAFKERMGWGLNWVSTAGSGFHRDLGFARTREELAPFLDK
                     GAIPATVEQNARMCGTDAAGYVAEGPGLSAYVMSEGIVYRTYVTTARGLEPVMGYYGL
                     LDRTARGRAEAGPQPHWVRRHDEY"
     gene            4769..5533
                     /locus_tag="VF517_17220"
     CDS             4769..5533
                     /locus_tag="VF517_17220"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX8054732.1"
                     /translation="MSAERGTELRLTALLLLGGAVAAWLVTVQRMHGMDMGPGTDLGG
                     LGWFAGVWAVMMAAMMLPSLVPMAGAYAGEARARPEAGEPGALVQTTLFVGGYLVTWV
                     LVGLAAWALVTTVDSLDVSFLAWHDGGRYLAGAVIAAAGLYELTPLKASCLRRCRDYG
                     LLVADWRGGPLGAARMGLAQGAYCIGTSWALMAALFALGVMSIAWMVVIAVVIAMEKL
                     LPRASVTSRVAAALMLALAAGVALVPEHVPGLTIPM"
     gene            complement(5732..6046)
                     /locus_tag="VF517_17225"
     CDS             complement(5732..6046)
                     /locus_tag="VF517_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX8054733.1"
                     /translation="MADKLRFTAVFEPADEGWIYAHVPELPEVHTQGRDLNEARMMLE
                     DAIRLVIADRHDRGEPIPDTSWAITETIDVSAALSDATSSGTSQLTGAARNVKAAGTH
                     SG"
     gene            6312..7388
                     /locus_tag="VF517_17230"
     CDS             6312..7388
                     /locus_tag="VF517_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054734.1"
                     /translation="MATFDVLGALPVTVDSCRFEHLRQVVATRRFERVTTLVRLGGGG
                     HEGIGEDVSLNAPDHDGLPDADALPVGGGPRPLREWLAALTPDAMWSSPPATDTGKRS
                     RQWAFESAALDLALRQAGLSLAAALEREPRPVRFVASFRLGEPPTLDPVRIRLDRYPA
                     LRLKLDPTTEWDESIFTSLADLDVVDVVDLKGAYKGTMVDQPADPRMYERALAAFPRS
                     LIEDPGLTPETEPILAPHRERVSWDAPIIGADDVRALDGRPAGINVKPSRIGSLERLC
                     GVYDHCRAEAIPMYGGGMFELGPGRRQIQALAALFHPDGPNDVAPSGYNEPEPPPGLP
                     VSPLPVALDAPGLGAEPPPGGALQ"
     gene            7403..8902
                     /locus_tag="VF517_17235"
     CDS             7403..8902
                     /locus_tag="VF517_17235"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8054735.1"
                     /translation="MTRAAEQVGERAAIKLGDRVTTYRGLDEASARAAALLRARGAVP
                     GDRVVLALPNSPWFPAFYYGALRMGAIAVPLNVLMREQELVACLTHCEARLLVAWHEA
                     LEHARAARASVDFDLIEVNPATADELLAAHPPERALADVAPDATAVLQYSSGTTGESH
                     AAELTHANLDASSRLMLEALGSVDGEVVLSAAPFAHSYGQNVGMSTILRGHGTLALMT
                     RFDAAEALEVVDRERVSIAVGVPTMCARMLELDPLPSRMPSLRLFLAAGGRMPVRLLH
                     EFERAFGCEVLEGYGMSEAPRIAVNRPGRRKPGSVGLPLRELEVRLLDANGEPAPPGE
                     AGELSVRGPNVMKRYWRRPAATAEMMRDGWLHTGDLALIDEDGYIHIVGRLKDTIVRG
                     GYTIYAAEVERVMATHPRVAEVAVVRVPDAEMGEEVGAAVVLVRGGDPPDPEELKGFV
                     RERLAPYKYPRVLWFAESLPRTATGKVIKRAIAIPPELVRSGEGAGGTS"
     gene            complement(8835..9728)
                     /locus_tag="VF517_17240"
     CDS             complement(8835..9728)
                     /locus_tag="VF517_17240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054736.1"
                     /translation="MKLQALPAAPYRIAREVLRRAPDPRVPEPTMLMEDPEAVEQFHA
                     SGPVNQVPLYQLCSRSTSMLAPAGATVLDLGSGSGRYLAHLARRRPDLKIVGLDLSEP
                     MLATGRRMLAEEGLADRVRLEQGDITEFAASAPEDVAVVSCIQALHHLPTDDLIRRCL
                     GQIAALRERTGCAVWIFDIGRAKHPMSYRGFISTTSPMPPVLEREAVQSERAALTVDE
                     LTAELERSGLGDLNHTHMRVLRVFQAHWAPHRDLPAGHENWEDVPLPDGTGMNTRMFL
                     TMFPRLPRPNGRAPAGSRSPA"
     gene            complement(9733..11370)
                     /locus_tag="VF517_17245"
     CDS             complement(9733..11370)
                     /locus_tag="VF517_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8054737.1"
                     /translation="MLLGAARREARDAAEARIGTILDEAAENHADRPMIFDRPPDIDP
                     EGPTSIDYARFAEFIAAVSGWLKAAGVEPWDRVAIFKGNHPDVLALTLACARIGAIPA
                     QLAWTHPPAVAHELLRRLERPVLITDRARLESAGLDDATLRELTKRTIVVDGEGEEGE
                     HVTPLAPFRGAPTPPPALRGDDEPMAIVHTSGTTGVPKLVVHSATTLGRAARYERKRI
                     PLLRARRDDTVAICDPYFHARLLGGLAAIAAAGPKLVLMSELDESSAKPVLDEHRPTI
                     VSTSPNAYLRWEWMTDEPSRPFKNVRLYFSTYDAIHARTVRRFLGASGHRFPVWFQAL
                     GQSEVGPVAVALHTRRSVRRHGEREPATQEFSIPVSFVGSRLRAVDPETGKPLRRGQA
                     GLLEVRREPCVDYLGEHGRHEHKIKETWWNLGDMGVVRRTGGVRLVDREVDRTPGASC
                     IGLEDVLLDRIPTLTETVVLGVKDGEPVPVVCTARDEPLDGDAWAEATRDLPALAEPI
                     VVPWDDIPRTATLKVQRSKLRERVLPNSAPAGSGLWT"
     gene            11498..12448
                     /locus_tag="VF517_17250"
     CDS             11498..12448
                     /locus_tag="VF517_17250"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX8054738.1"
                     /translation="MTAPASAPARGFRERFVAYKDLAKLEMFYPFLAVPLVWTLLESE
                     VANDSTTLVIVVLAVVVVVGVRTAESALDDVAGIRDGIDTFNYEQPSSDRRRTRKPLL
                     DNRLTEQQAINYARIALIASLASFVAAGIIVDFDPLWAPIAAVVAAFAVVAYSWGPKL
                     SYIGAQELIAVLGVAAVVCVTYAYATGGLTWSAAIEGLLLGLWLDQTVVLANLHDLEG
                     DRRAGRGTMAVRLAARPYQRYVIAVFALAWTLLLAAVILGELPWWLLLLQIPVVALQV
                     RILVVGIRHDDPLSGWRLGRRTFPIAWMTLVVANLIAFHW"
     gene            12514..13314
                     /locus_tag="VF517_17255"
     CDS             12514..13314
                     /locus_tag="VF517_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8054739.1"
                     /translation="MEASSKQLAVVTGASSGIGLELAKLFAANDFDLIVVAEDPDIQV
                     AAQGIRDSGAGARAVQVDLATPAGVEELHKEIQAEGRPVDAIALNAGVGAGGAFATDT
                     DLEDELRLIDLNVRSTVHLAKYVVRDMVARGQGRVLVTSSIGATMPGSFHAVYNASKS
                     FVQSFALALRNELKDTGVTVTTLMPGPTDTEFFERADLEDTPVGSGSQDDPADVARGG
                     FDALMAGEERSVPGSLSTKLQGRFSRVLPDSAKAAMHRRMAEPGSGDE"
     gene            complement(13342..13677)
                     /locus_tag="VF517_17260"
     CDS             complement(13342..13677)
                     /locus_tag="VF517_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054740.1"
                     /translation="MSSNAVADLVGAIGSMLLRRGAWCVQVGGESSVRKGTPRILACY
                     GGRFIAVEVKAPRGPGPTQTQRVQLEALDRAGAITLVARSVCEVEAVLEDLDRHPPEM
                     LALERMITA"
     gene            13746..14312
                     /locus_tag="VF517_17265"
     CDS             13746..14312
                     /locus_tag="VF517_17265"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX8054741.1"
                     /translation="MAEPELTDAVSIVLAALAVVLQALLAGLLALGLAALASPRARRW
                     LAELRATLAGGELWAAWAVALVATLGSLFFSEVSDFVPCRLCWFQRIAMYPLAVVLLL
                     AAVRRDVRGGALYALPFPVAGALIAIWHVYVEINPDAESEGCRVAAPCSLKWIDELGY
                     VTLPVLSLTAFAAILALLLMARSRPADA"
     gene            14305..14919
                     /locus_tag="VF517_17270"
     CDS             14305..14919
                     /locus_tag="VF517_17270"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX8054742.1"
                     /translation="MPDLLLPYLAVIAVLTITPGPDMLLVLRNGVRDGTAAAWATGLG
                     CCTGIAVHAALAVLGLSAILAASSEAFTVVRLAGAAYLVALGVQAIVRSRGAHAVAEE
                     RAEEPGSAYRQGLLTNLLNPKIALLFLTLLPQFVADGEPRVATTAVLAAIFLAGALVW
                     WRAFSLLVGPLGRFLSRPRVVAWFDRGTGALLVAIGVRVALERR"
     gene            complement(14815..15147)
                     /locus_tag="VF517_17275"
     CDS             complement(14815..15147)
                     /locus_tag="VF517_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054743.1"
                     /translation="MAALRERDLTGVPALDLIGNALQLAHDFGQPHPEPVPKSGGGGG
                     ASAALVGGGVAATLAVAGGLIWVRERTRRADASTALEGDAHADRDEQRAGAAVEPRDN
                     PRAGQEPA"
     gene            complement(15120..16544)
                     /locus_tag="VF517_17280"
     CDS             complement(15120..16544)
                     /locus_tag="VF517_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054744.1"
                     /translation="MTARRAALPVAAAAVILAAVPQPALAHGIQVKRDLPVPTWLFVW
                     GAALVLLISFVALAVLCPKPRLEDGEWRPLGRAGRLLASRPVEVACGAIGALLLALVV
                     YSGLAGEETDNLAPTWVFVVFWVALVPVSVVFGDVFRAFNPWRAVARAASWAFRRAGR
                     EPSEPFDYPDRLGHWPAAAGLLAFLFMELVVTAGNEPRSLAVAALVYSAATWFAMSVY
                     GIECWLDRGEAFSVYFGLFARLSVFERRGDAVGRRRLLSGLGGVLPLAGTVPLLAVMI
                     GSTTFDGLTTKGVWRQDLEPELIDAFSSLGMSGATAGQFAAAVGLFVVIGLVLGFYRL
                     GIVGVRTAGGDLSRERLARAFVHSLVPIGVAYVLAHYVSLLLIQGQAVPALLSDPLGS
                     GSDLFGFAGWTVDSNWVASETIWYLQVTFVVLGHVAALALAHDRALALYDRASVAVRS
                     QYWMLGVMVGFTCLALWLLSESGT"
     gene            complement(16541..16996)
                     /locus_tag="VF517_17285"
     CDS             complement(16541..16996)
                     /locus_tag="VF517_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054745.1"
                     /translation="MDGRRATILIVTAVLAVAAFLVLRPSGDDDDDSKRGTPTAQPAP
                     TTTTTTPAETQPAPEAQPKEPAVKLIRVRGGRPVGGVQTIEASEGDTVRFRVQTEAPQ
                     EIHLHGYDISEETTADRPAEFQFKANESGIFEIEVEDTATQIAELKVQP"
     gene            17131..17436
                     /locus_tag="VF517_17290"
     CDS             17131..17436
                     /locus_tag="VF517_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054746.1"
                     /translation="MIGYLIALALTGLIVGALARLALPGKDPMSIPMTMLVGIAGSLI
                     AGLVYYAITDDRGGLGILPAVLVSTGIVYLIRRSRGGTLTQAAPRDGVGGRQRPLTR"
     gene            complement(17485..17958)
                     /locus_tag="VF517_17295"
     CDS             complement(17485..17958)
                     /locus_tag="VF517_17295"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8054747.1"
                     /translation="MAENHAYIDAPPDAVFDVLADAPSYADWVVGASQIRDVEGDWPE
                     AGTKFHHTQGAFGIGLKDSTTAVAANRPRQLVIVVRFRPFMIGKVEFRLRPRGRGTHV
                     TMIEYPIGGPVAKVNNPLIDRALWARNVETLRRLRRISEQRAARTGEVPARAAAA"
     gene            complement(17993..18928)
                     /locus_tag="VF517_17300"
     CDS             complement(17993..18928)
                     /locus_tag="VF517_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054748.1"
                     /translation="MRRLLPALSLAAAALVIAVPASGGPVKGAPKCRILPADNHWNMR
                     VDKLPVLPNSDAMVRGIGADGTLHPDFGSGLYEGRPIGIPYTTVSKSQKRVKVSFEYA
                     DESDRGTYPIPPKAPIEGGSSADGDRHVIVVDRDRCKLYELYAAYPRDGGRSWRAGSG
                     AIFDLRSNRVRPAGWTSADAAGLPILPGLARYEDVKRGKIDHALRFTVSRSRRAYVYP
                     ARHFAASSTDPDLPAMGQRLRLKASFDTSGYPRQARVILNALKRYGMIVADNGSDWFI
                     SGAPSKGWDNDELNALKGVEGSNFEVVDSTRLPRP"
     gene            18961..19140
                     /locus_tag="VF517_17305"
     CDS             18961..19140
                     /locus_tag="VF517_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054749.1"
                     /translation="MPEREESDQLPEEGQGGEVHDDDGGQGASREDAEDQAGAADDDG
                     DEGEGQATGNPRSAG"
     gene            19085..20353
                     /locus_tag="VF517_17310"
     CDS             19085..20353
                     /locus_tag="VF517_17310"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX8054750.1"
                     /translation="MTATRARARPPGTRAAPASRVPLASRLDPSAAREAWRAFVESRV
                     VVLVAAVLGVALFPLSGRAQAGHPDIVHPFEGWPLGELLDSLVAPLIRGDASWYMMIA
                     HDGYVDEGIQTGGLVQGRPAFFPLYPLLMRALGGFGSFGFAALAGTLISLASLLGALY
                     LLYRLTELELGPGAARATVRLIAFAPVAYFFSAPYTESLFLLLSVASIYAARRGGWAA
                     AGLLAAAASGTRNVGALLIVPLALMYLREHGRPGPRMAWVGLAPAGLVAFSVYLRADV
                     GDWQAWRHAQVYFGRPETVNPFEGARQGVAAAWDALAGSAPDDLQIPMLVAFGFLVFA
                     LVALVGVFRSLPLAYGAYSLALVIPALCLPVLDNPLYGFPRYTLVAFPLFMWLGLRCE
                     RAGVTDRVVLAFACLLAVFTAGYATWQPIG"
     gene            complement(20350..21078)
                     /locus_tag="VF517_17315"
     CDS             complement(20350..21078)
                     /locus_tag="VF517_17315"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8054751.1"
                     /translation="MKPVRVGCSGWNYQSWRELVYPKGCPPSRWLEHYATLFDTVEVN
                     STFYRLASRDAVARWVEQTPPDFVFTLKMSRYITHIKRLTSLDTGIDKFYERVEPLTR
                     TPKMGPVLWQLPENFHRDDDRLAAALKALPSGRHTFEFRHTSWFVPEVYELLREHGVA
                     LTIGDHPERPFQSYEMTADWTFVRFHYGHRGRRGNYSESELAEWAERIAEWRRRVEVF
                     AYFNNDWEGFAVRNGLWLRERLGV"
     gene            complement(21100..21333)
                     /locus_tag="VF517_17320"
     CDS             complement(21100..21333)
                     /locus_tag="VF517_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054752.1"
                     /translation="MSIGLGILGLIILGGLVGSLAFGAPYVGIPLALIFIGAVIGKEQ
                     MERQNRIMRMKRFRREARAQPVDFDARDKTTVV"
     gene            complement(21334..21786)
                     /locus_tag="VF517_17325"
     CDS             complement(21334..21786)
                     /locus_tag="VF517_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054753.1"
                     /translation="MFRKLSLLALLGGATAFLLKKRSGGGGQEAQQSAPMPSTSSAQA
                     PDVQVNEQQAPDTTAEDLAEVRPAQTEETIEAPIAGIGDDEQAVIPDISNDDQLVREQ
                     EAAAAADAGSIGGDADTVTADAPEEMRPVVEGAGDAPETFETTDEQGR"
     gene            21879..22496
                     /locus_tag="VF517_17330"
     CDS             21879..22496
                     /locus_tag="VF517_17330"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX8054754.1"
                     /translation="MLETLKPILRAAAIAGFTVGLILFLVNPPSLSGIQGLLVWTAGA
                     VAMILMGAWAAVALTGNESMTEEEVERLAVRAEDLAGRGAGGGGGAGSASAGGDGERG
                     PTDFDTLVADAIDRLPPEFLDLLARVPVIVSHRGREFRAYGHHYGGPYTRGDYEHRIV
                     IYQDTLERDFGHDPELLAAQVERTLRHEVAHHIGWDEPGVRGLGL"
     gene            22631..22719
                     /locus_tag="VF517_17335"
     tRNA            22631..22719
                     /locus_tag="VF517_17335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22665..22667,aa:Ser,seq:gga)
     gene            22981..23484
                     /locus_tag="VF517_17340"
     CDS             22981..23484
                     /locus_tag="VF517_17340"
                     /inference="COORDINATES: protein motif:HMM:NF025381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEX8054755.1"
                     /translation="MLSDRLREKVRISFPQDPGREERPEAFDDWLRLRFQVPLERDAL
                     AGPAFGKSATALGVATIGAGLASSAITTASGGDWATVVVASLGIIVGVLATINQIWRP
                     SERSVARYQAAFALRREGWAYLQDRGRYERVAPEDRLPAFIDEVTRIHRAVELVDEAT
                     SASQTGG"
     gene            complement(23780..24421)
                     /locus_tag="VF517_17345"
     CDS             complement(23780..24421)
                     /locus_tag="VF517_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054756.1"
                     /translation="MSDEQPVEPQPEAMSTEQRLEQVLYQLADEIDDHGDDPKLRWSR
                     QVREAADLIGRGEAWGYRLFFALFEEVGRPGDHWPPIPNPFTEQPFARTGTCDEALRL
                     ADEVLRENRERSRDAPEREIVLRPWTEGSSGKAVVYEDGTVVATQDDAGGGPRIPDIK
                     AASRPDEDPVAELAIRPNGLCATYRHRCDRPWLTARLREHDPGLRLEQKPPRG"
     gene            25438..25710
                     /locus_tag="VF517_17350"
     CDS             25438..25710
                     /locus_tag="VF517_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054757.1"
                     /translation="MPDTYAQKFADQGSVALAFDYSHYGESAGEPRQLELPAEKLSDL
                     EAAVSVPARERALPPAGEVVLDVPALAADQAARGTTVVGPPMGAGD"
     gene            complement(25760..25906)
                     /locus_tag="VF517_17355"
     CDS             complement(25760..25906)
                     /locus_tag="VF517_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054758.1"
                     /translation="MTSPMGRVIVEMLVDALTTAAVPAHVAGYRPELHTPSTAWVVLG
                     EQDG"
     gene            complement(25903..26310)
                     /locus_tag="VF517_17360"
     CDS             complement(25903..26310)
                     /locus_tag="VF517_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054759.1"
                     /translation="MHALVRLAVREGDAATARWLLQDAERRYPDAPARALISVAPDVL
                     GPRLRLDGESPEFDEHPGTGTFTVITPHAQRAAAALGPVLPRLLHVDERGTVMSWEER
                     EGTEHEEVYTPNHVFDAEWSEHGARVSLDRRDR"
     gene            complement(26470..27363)
                     /locus_tag="VF517_17365"
     CDS             complement(26470..27363)
                     /locus_tag="VF517_17365"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8054760.1"
                     /translation="MGLLRRSVTICALAGAFLLCTGASASADTPLRFWHFNMCGAVIK
                     APCNGGAEQPVADAIKNSIQNWKPDGVSLNEACQSQWATTYWNLNAAGLWSMGSRFVT
                     TRYLSAPTSTSTINCNLYDNDRRFGNALFSRQTILMEPSATSLPKTQSTAETRKLWCM
                     VVDLSRDTVYCSVHITSYDADIKAQIDRVASTVNQWVDSGTPVVLMGDFNVEPNDDRL
                     DPIYHSVLGGGATGRFEEVDEYNGSTTRCRCGEATHGDRKIDYIFVSARDFYAVDGDA
                     TSSSYSDHDPLRGSATLDDTP"
     gene            27447..27659
                     /locus_tag="VF517_17370"
     CDS             27447..27659
                     /locus_tag="VF517_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054761.1"
                     /translation="MATDQTVLAKTGRVTGRVAPGTVGEVVLPIRGGSEAFYAYAAEP
                     DQVIEPGTRVVVIDFEEPRTVRVLPV"
     gene            27672..29144
                     /locus_tag="VF517_17375"
     CDS             27672..29144
                     /locus_tag="VF517_17375"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8054762.1"
                     /translation="MSVLITIGLIAAVPLVLAAVVALIVKLTWRVAEPNEALIISGLG
                     ASSRQALDSGLGFKIIVGKGTLSLPGLQTVRRLGLELHKADLTVDCVTTQGIPVHVRG
                     VVVYKVGDGTAEIANAARRFLEKEHEMDANVHEVFAGHLRQIVGAMTVEDMIRAREKL
                     AAETRSSSAIEMEKLGLVIDSLQIQQVEDPTGYIENLAKPFQAAVEADARIAAAERNR
                     EATEREMAAAAVAAKAVSDSEIEQARLRAAANTATAQADQAGPLAEAESRKSVVVQET
                     EVAELEAARTEKRLQTDVVKPAEADRDARIAQAEAEKREVELAAAAHAEKVKIEAAAN
                     AEQVKLKAGADAEQTRLNADANAIATERTGAAEAKATTLKGEADASAVKAKGLAEAEA
                     IDARAQALERNQDAVIGQQVAEQLPEIVRAASESFRGIDNLTVLNGAAGMNDMMTQIM
                     SMGASAIPMFRSMLAGNSAAPDDNGAGTAALGTSKGDDKT"
     gene            29171..30193
                     /locus_tag="VF517_17380"
     CDS             29171..30193
                     /locus_tag="VF517_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8054763.1"
                     /translation="MDQRTLAGTGVHVSPLCLGAMMFGAWGETDHDAGIQVIHRALDA
                     GINFIDTADVYSRGESEEIVGKALAGGRRDNVVLATKFHGTMGDDPNEAGNSRRWIFR
                     EVEASLKRLQTDWIDLYQVHRWDPWTDHDETLGALSDLVAQGKVRYIGSSTYPAAQIV
                     RAQWVARERGYQRFVCEQPPYSLLVRGIEADVLPTCLEHRMGTIVWSPLAGGWLSGRW
                     RKGADDLTSRRSAMIPQRYDLSIPANQAKLDAADALGRLADEAGITLVHMAIAFAIRH
                     PGVTAAIIGPRTMEQLESQLGAVDVQLDDELLDRIDAIVAPGTTINPADAGWTNPALA
                     AEARRR"
     gene            30289..32373
                     /locus_tag="VF517_17385"
     CDS             30289..32373
                     /locus_tag="VF517_17385"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX8054764.1"
                     /translation="MGRRARFGFATAIVCAAIAAAPAAAGAATPAPTPVTGTSSAFAD
                     SIGINAADFYPDTLYANHALVKQLLLDLGIRHLRGGIRLGRSAQEYADVRDLAAAGLK
                     KMWITGRPDSTQGDPTNFSQVRDILTDPNRLGGTTEALEGPNEFDSRASEDSLWADKL
                     HVFQTQTYTWANSDSTFSNLPFYGPSFLSQSGRQTYASKPGANNVMDAPNAHPYAGGR
                     MPEDRIAAEISELAGLFGNRKPVISETGYHTALNGGNWWHYGVSERAQSIYLMRTLLT
                     AFALGAPRTYIYHLLDLKPNPAQDDLEMNFGIVATEGDPAQSAQPTWTARPKQAYNSL
                     KEVFDLTASSGSVDAGPTRLSYALTGAPSTLRKVILSRRDGSTDLVLWNAVPVYNDRS
                     YDCNDPRVQQYPAQDRCFWAMLLYSQDQGDAFPADVPVTLDLDDTARVSTERPHSQTS
                     FTALGEGKTFNLNVGADPVFVRIEPRYGMAVRADQPAAWLRLGESSGAPGDSAGRGAT
                     TTGWTSAPTYGRPGAVGDASTAVGVSGSQRATVNLASPVSTDTGTTVEMWVRPDTTTP
                     DFTEFLTSNSPDVRVRMRAYNRHDSRDWGSTYGSRPGATGEMIFPGFAYGQWHHVVLT
                     MAAGGSVTYLDGKKVGETTGGEALNVSAFTVGANGGNGGFKGDVDELAVYPAALSAGR
                     VCAHYRAAGGSC"
     gene            32460..32951
                     /locus_tag="VF517_17390"
     CDS             32460..32951
                     /locus_tag="VF517_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX8054765.1"
                     /translation="MSSVDRLLENSRAYAAGFAKGDLAAPPAKRVAVLACMDARLDPA
                     RLLGLEEGDAHVVRNAGGAVTDDAIRSLAISQHLLGTEEIVLIQHTHCGMQGASEDEM
                     AEALERASGERPPWPLHAFEDLETNLRESLRTLRESPFILDSATVRGFVYDVKTGELR
                     EVQ"
     gene            33002..36232
                     /locus_tag="VF517_17395"
     CDS             33002..36232
                     /locus_tag="VF517_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054766.1"
                     /translation="MPPVRAVLLAAMLALALAAPAAAAPFQLTPTGGDPDVAVDSSGT
                     AHVVWNQPSSEGSDVTVYCQLPRGATTCSRFQNLIPAGEKLFDAAGPQVVLAPDGRIA
                     VATGRCCSQVWRFVSADGGQTFDSRLVGDLTGVSDWRAAFGPGERISLVNAGTYQAPP
                     LDGSTNAKAELLPGSIYGTGLTMLDSNTPLVVASMTGERDQLGWRRYDGSGDPNDAGN
                     WTPAQPLGPGTDPHLVTGPRGTYLIYEAGVESNAAYVIRRWNGAGFDPVAATVISAGG
                     QQRSPAFHQDPTGTFHAVFGSNDTMPQRLLYSSSPDGTTWSAPREIAPYRESGYFHNE
                     IAAAGPDDGLAVNDVNESAGNVEAAPLSAQAAEPERPDDPSCRTRAEFRPVKVVLPEG
                     CFKQVSGRLETSDKVKVNGLLIEPRAGATVVIDQAKRRIHSRGVTTVRAGLIPVQVGV
                     LDWSIKDAAKSSVDTFDLGDAGGKVFGFPLTATGEVDFDSGTTTIAAHVGLPKFLGGV
                     TGDITLRTDNDNGLRLDGLVIKVPSALIGPVQVKGLVLSYMAAGSVWEGSATLLLPPS
                     PPGPKLDAAIGFREGGFDYGRGEYTFTSPGLGLGAGVFLNKIRFAVNLQPAPPRIAGG
                     VTLSAGPTVAGQKALGIEGDISYTFPNPPAPAVLRADGTATLSSIPFLKAYAEYRSSG
                     FFAFGAELNYFFVKDLVGINAAVDGWFTSSAFNIEGKGTVCVGYCFGAEAVLSSEGIA
                     ACGRVPVWGTEVALGFGYKWSGGLSIMLLSCDVGPFRAVQSAQAGGESEFTLGSGLPV
                     ATVAFRGATAPPKVVLEGPGGERVETPAAADGKVLDERFLLFQNPDDKTTYVAINRPS
                     AGRWKASVAGAAVSPRLDVRAAQSSPIADIQVATGLPKPSVKARVSGSGHSRRLTYSV
                     KSIPGQKVHFEEVAATGGGRRIGTAKGKTGTLRFSPADGPKGKRRIVALIESNGIPRE
                     NIDVATYTAPAPARPARPAGVKLQRKGSRVTVTWRPAARSARYLVRANLSDGRRLLLF
                     ATAKKRSVRFTGLPTSARGTITVVGVKPDGTPGSAARAKLAPRKRR"
     gene            36323..36856
                     /locus_tag="VF517_17400"
     CDS             36323..36856
                     /locus_tag="VF517_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054767.1"
                     /translation="MAGLRGVAIASGAAALVLAAGCGGGGAKKQEKPATPAETPVNDP
                     PVQAKESGAPSDLLGQYTVKLPKADLPKDAPEELTEGSETWTVTLAETGGPGEGPAFT
                     IANDQLGALESSNFEVKGDRILLHEEECAQTGEPVESEWTYELKGERLTLKEAKGACP
                     DEVASTILTTSPLQRKK"
     gene            36869..37027
                     /locus_tag="VF517_17405"
     CDS             36869..37027
                     /locus_tag="VF517_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054768.1"
                     /translation="MRIPWKLIGLAGLAGVAATGVVVVRRRRRAQRDYSPDELRARLH
                     QRLAEVPH"
     gene            37042..37617
                     /locus_tag="VF517_17410"
     CDS             37042..37617
                     /locus_tag="VF517_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054769.1"
                     /translation="MAEAATGDIWLFRGRSLADRAIRTVTNSPVNHVGMVVAIDDLPP
                     LLWHAELGRSLPDVWTGEHQRGVQLHRLGEAVRTWNERYSQRAWFRPLEGTVERSHED
                     RLMEVIERFDGRPFPTTLGLARQWVNGRFRRSSSREAIYCAELVATTYQHMGLLPSRR
                     PASWYDPGRFWSGDRIELVAPFALAGEVAVD"
     gene            complement(37693..38157)
                     /locus_tag="VF517_17415"
     CDS             complement(37693..38157)
                     /locus_tag="VF517_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054770.1"
                     /translation="MLLFVALAVMAIAATPVVAGAAPTFEKADGTGESPEYGAVHVNA
                     KDQGDGPRGHWFVSDAVGTPFQGDVTCLRQEGNTALVGGLGRNEVAPGVLLPILIEVV
                     DNGQPGHGADQHRWRFAFPDEVADPDCADFGDNPPRANIVRGNYVVPTGSGG"
     gene            complement(38254..38532)
                     /locus_tag="VF517_17420"
     CDS             complement(38254..38532)
                     /locus_tag="VF517_17420"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054771.1"
                     /translation="MDMRHEDIILETDRYRIEGKLTLPREGYRSRLSDYVNQRDREFF
                     ALNDATVTALDAPEQTRTASFLMVARSHVRIVALAAEESFGAGIGEAA"
     gene            complement(38543..>39482)
                     /locus_tag="VF517_17425"
     CDS             complement(38543..>39482)
                     /locus_tag="VF517_17425"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8054772.1"
                     /translation="TEGGRFLIDVATTAIFSFEPRDMSMLFVLFYIAAAGTEGTPGTL
                     DRLVNTAGGAQELRFHGGSQRVPLELARRLGKRIRLNAPVRRIVQRKGSVEVHANGVT
                     ATGQRVVVAIPPHLAGRIDYSPKLPGMREQLTQRMPMGSVIKVQAVYERPFWRDRGLN
                     GQVVSDTGPVQVTFDNSPPDGSRGVIMGFVEATTARRLDRTGPEERRRLVLENYASYF
                     GEEALNATGYVEALWDSELWTRGCPVCAAPPGVLLDYGEAIRAPVGRIHWAGTETSTY
                     WNGYMDGAVRSGERVARELLPRLGRSVCTPVRSGRR"
BASE COUNT         5639 a        13939 c        14183 g         5721 t
ORIGIN      
        1 cgactactcc ggctaggtca gtcggggctt cgcaacgcat tggacggacc aacgcgttgc
       61 ttgcgcgcag cggggcccaa tgcgacctca tgctcaggtt gcgtgatcga ccgcttcggt
      121 cgcgcgatcg acgcacgcca gcattgcggc gcgcagatcg ccgccccgtt cgcggcgctc
      181 gtcgaccgtg tggccgtcgc taagccatcg cccttgaacc cgtctcggcc gtaatgacgc
      241 accggccagg agtaccacga ccgaggaccc gccgtagagc ttgtgccgac cgtcgacgcg
      301 gcgcccaaag agcgtcccct cacggtcgag ccagcgatcc tcgccggcgc tccaacccgg
      361 ggcgcggccc tcctcgatga acttccagaa gacgctcttc cagctgtggt agttgtggcc
      421 ggtgcgctcg cggatggcga cgaactcagc gtccggggca atcgtctcgg cgacccactc
      481 gcgccagacg gacagacacg tctcccgcgt gagctccgtc tcgtaccccg cgaggcaatc
      541 cctcacctcg tcaagggaag cggcttcgcg gagcgccgcg tgtgcctcct tgaccgccgc
      601 acgccggcct gcggctgctg ccgcctcatg ccgccggact cgctcggcgt gctcggcttg
      661 gcaccagggg cagatgcccg cgtgggagta gcagaggcgc cgaccacatc cgccgcggca
      721 ggtcgacagg gaatcgaagc cgcagccacc cacagcgcac ggaatccctg acgcacggcc
      781 gtgctgaaag cgctggccgc atgtgcgagc gccgtggacc cattgaccac cggggccccc
      841 gggatgggac atgtccacca agctggtgca ctcgaacagg cccggcgcga cctgcttact
      901 agagccctgg aagacgggca tgcggcagcg caagcagagt tccatcgcgc caccctaacc
      961 cggcgtcgcc gccgcgcgcg gctcgaatgc gccgcgggtg tcgccgtgcc ccgcgtgacg
     1021 ggctaggagt tcgctggggg tcagccgcct gcgagctgcc tcacgtcggt agcgcgtctg
     1081 tgacgcctga actacagctt tctgcgcacc ttttcgttgg ctgtcccctg cgctccggtc
     1141 cgttcgtcgt atcgacgacg gcgcttgacc gccgtgatcc ggcaccgacg agaaaggcat
     1201 gacatgaccg agagctccac catcgggcgg gacaccgcga tcgcggaggt ccgcgccgtg
     1261 ttctccgaga tctatgccgc gtgggctgac aacgacgccg aggcgttcgc ggccggctat
     1321 cgcgaggacg cgacggtcgt catgcccggc gtgcggcaca tcggcaagtc ggcgatccgc
     1381 gactccatgg ccgccgcctt ccagggtccg ctgaagggct ccaaggcggt cgacgagccg
     1441 gtggacttcc gcgtggtcgg cgacgacacc gcgatcgtca tcagcaaggc tgggatcctg
     1501 atggccggag agaccgaggt gcccgccgag cgcgaagcga ccgcgacgtg ggtcctgacc
     1561 aagcaggacg gcgagtggct gatcgccgcc tacgcgaacg ccccggctgt cacctagtct
     1621 cgaacgtcga tgcgcgaccc gaagagacga tgatgaagta cgccctgctc atccacgagg
     1681 gtgtcggcgc gtacgagcag ctgagcgcgg acgagctcgc cgccgtcacg gcccagtacg
     1741 tggagatcgt ccaggacagg cgagtgctca gcgccgagca gctggagccc ccgacgaccg
     1801 cgaccaccgt ccgtgtgcgg gacggtgagc tgctgctgac ggatgggccg ttcgccgaca
     1861 ccaaggagat cttcggcggg tactacatcg tcgaggcgcg cgacctcgac gatgcgctgg
     1921 agctcgcggg ccggatgccc gccgcgcggc tcggtggttc ggtcgaggtg cggccggtgg
     1981 tggagcgcta gcactgctgg agcgggtctt ccgtgacgag tgggggcgtg tcctcgcgtc
     2041 gctggtcggg tacctcggcg actttgagct ggccgaggac gccgcgcagg aggcgttcgc
     2101 catcgcggcc gcgcgctggg cgcacgacgg acccccggac aacccggggg gctggttggt
     2161 gatgaccgcg cgcaaccgcg cgatcgatcg cctccgtcgt gagcacaccc tcgcacggaa
     2221 gacctgtcag ctggagctgc ccgaacccgt ggagctcacg ttggacgacc cgatgattcc
     2281 cgacgagcgc ctcgagctga tcttcatgtg ctgccatccg gcgctggcga cagaggtaca
     2341 ggtcgcgttg acgctgcgtg cgctcggcgg gctgactacc cccgagatcg cgcgcgcgtt
     2401 cgtggtctcc gacgagacga tgaagcggcg cctgtcgcgg gcgcgcacga agatcaaggc
     2461 ggccgggatc cctttcgagg tcccggccga ccgcgtgcta cccgaccgcc tgcgcaccat
     2521 cctggccgtg gtgtacctca tcttcaacca gggctacggc ggcggccgcg tcgacctcgc
     2581 cgacgaggcg atccggctgg gtcgcgtcct cgtggacctc acgcccgacg agcccgaggc
     2641 ggcagcgctg ctggccctca tgctgctgca cgacgcgcgc cgcgacgcgc gcttccgtga
     2701 cggcgagatc gtcgtgctgg gtgaccagga ccgcacgcta tggaacgccg agcagatcat
     2761 cgcgggccgt gcggtgctcg atcgcgcgat cgcgctccgc ggacgcgggc cctacacggt
     2821 ccaagcggcg atcgcctcgc tgcaggtcga gccggcgatc gactggccgg ccgtcgtcgc
     2881 gctctacggc cggcttggcg agctgaccgg atcgcccatc gtcgcgctca accgcgcggt
     2941 cgccgtcgcc gaggtagaag gcccacacgc cgcgctggcg atcgtcgacg cactcgagat
     3001 ccccgacgac taccgctacc tccactccac acgcggggag ctgctgcgac gcctcggcga
     3061 ccatggcgcc gcacgagacg cctaccagcg ggcgctcgca ttcaccgtca ccgaccccga
     3121 gcggcgcttc ctccagcgtc ggctgcgcga cctctcggcg gggcagcctc ttcgaggcgg
     3181 cggcgatgaa tttcggccca actccgggtc gtagctctag atccgtagtt ttccggcgac
     3241 ggcgatccga ccgggccgaa gcggtcgagc gtgaaaggag cggtcaggtg tccgagcaca
     3301 agactggaac ccgtgaggag tggcaggccg cgcgcgacga tctcgccaag ctcgaggccg
     3361 agcacgccga acgcaacgag gagatcaagc gcaagcggcg tgagctgccc tgggtcgcgg
     3421 tggagaagga gtacgagttc gacaccgagg acggcaagaa gaagctggcc gacctgttcg
     3481 acggccgctc acagctgctc gcctacaaca tcatgtacgg gcctgactac gagctcgggg
     3541 cgtgtccggg atgcacgagc ctcgccgacg ggttcgatgg gacgctggtt catctgaacc
     3601 acgccgacgt gacgttcgtc ggcttctcgc gcgccccgat cgatcggctc gtcgcctaca
     3661 aggaacggat gggctgggag ttcccatacg tctcgacgta cgggagcgac ttcgcgttcg
     3721 acttcggcct ggccctgagc gaggagcagg cccggcagat cccagaggtc cagcagatgg
     3781 tcgacgaccc acccgagtgg ctccagttct ggtcgacgca ggtcggcgcc gacctgaagg
     3841 acggactccg ggaggccccg ggctacatcg cgttcgcgct cgagaacggg accgtctacc
     3901 acacctacac cgtgacggcc ccggacccat tcgtgtcgcc gttcttcggc ttcatgctcg
     3961 accgcacacc caagccccag cccgaagagc cccggacctt ccgcaaggac gaatacccgg
     4021 actgacagga gcagccgatg cccgaacacc gagtggaaac gcaggagaag tggcaggcgg
     4081 agcgcgacgc gttgcttgcc agggagaagg agctgaccaa actcagcgac gagctggcgg
     4141 agcggcgtcg tgagctccct tgggtggccg tggaggagga ctacagcttc gagaccacgg
     4201 agggcaccaa gtcgctagcg gagctcttcg acgggcgctc gcagctgctc gtctaccact
     4261 tcatgttcgg tccgccctac gagtccggct gcccggtgtg ctcctcgatc gccgacaacc
     4321 tggaccccaa cgccgtccac ctggcggcgc gcgacaccac cctggtgctc agctcgcggg
     4381 cgccgctcga gaagctccag gccttcaagg agcggatggg ctgggggctg aactgggtct
     4441 ccaccgccgg cagcggcttc caccgcgatc tcggatttgc gcggaccagg gaggagctcg
     4501 cgccgttcct ggacaagggc gcgatcccgg cgaccgtgga gcagaacgct cgcatgtgcg
     4561 gcacggatgc ggcgggctac gttgccgagg gccccgggct gagcgcgtat gtgatgtcgg
     4621 aggggatcgt gtaccgaacc tatgtgacga ccgcgcgcgg gctcgagccg gtgatgggct
     4681 actacggcct gctcgaccgc accgccaggg gtcgcgcgga ggccggaccg cagccgcact
     4741 gggtgcggcg gcacgacgag tactaggcgt gtcggccgag cgcggaacag agctccggct
     4801 gaccgccctc ctcctgctgg gcggagccgt cgccgcctgg ctcgtgacgg tgcaacgcat
     4861 gcacgggatg gacatggggc cggggactga tctcggcggc ttgggctggt tcgccggggt
     4921 ctgggcggtg atgatggccg ccatgatgct cccgtcgctg gtgccgatgg cgggcgccta
     4981 cgcgggggag gcacgggccc ggccggaagc gggagagccg ggggcgttgg tgcagacgac
     5041 gctgttcgta ggtggctatc tcgtcacctg ggtcctcgtc ggtctcgcag cctgggccct
     5101 ggtgacgacg gtcgattcgc tggacgtctc gtttctcgcc tggcacgacg gtggccggta
     5161 cctcgccgga gcagtgatcg cggcggccgg cctctacgag ctcaccccgc tgaaggcgag
     5221 ctgcctgcgg cgctgccgcg attacgggct gctggtcgcc gactggcgtg gaggaccgct
     5281 cggcgctgcg cgaatgggcc tcgcccaggg cgcgtactgc atcgggacct cctgggcact
     5341 gatggccgcc ctcttcgcgc tcggcgtcat gagcatcgcc tggatggtgg tcatcgccgt
     5401 ggtgatcgcg atggagaagc tcctaccgcg ggcctcggtc accagccgcg tcgcggcggc
     5461 cctgatgctt gccctcgccg cgggcgtcgc tctcgtcccc gagcacgtgc cgggtctgac
     5521 gatcccgatg tagcgaacct gcaccggctc ggcgctctac ccctgctggc gcttgagact
     5581 gggcccgcag agtccgaatc cgagcatccg cggcgatgcg cctaccgaaa cggcggcggc
     5641 gggacaccta gctggcggca gatccccctg cccgtcggca tcttgatttc gctgtggcga
     5701 gggagcacgg cgcgttggcc tgtcaccgca ttcagccaga gtgtgtgcct gccgccttca
     5761 cgttccgggc ggcacccgtg agctgcgagg tgccgctcga ggtcgcgtcg cttaaagcgg
     5821 cgctgacgtc gatcgtctcg gtgattgccc agcttgtgtc gggaatgggt tcgccgcgat
     5881 cgtgccggtc tgcgatcacc agtcggattg cgtcctccag catcatccgc gcctcgttga
     5941 gatcgcgccc ctgggtatga acctcgggga gctccggcac gtgggcatag atccagccct
     6001 cgtcggctgg ctcgaaaaca gccgtgaagc ggagcttgtc ggccatcgct caagagagta
     6061 cgcccgagcg gcggcaagca caaagcacgc gcccgtactc gggcgtcgcc cccgaccagc
     6121 cgctgcgcgt ccgcccgcgg tctccgcgac cggggtttac gcccattccg gcttggggta
     6181 gagccaacgg aggggaaccc ggcgcctgtt cacggccggg atggggggag gtagctcggg
     6241 agtgctcgac cggctgacgg tttcacgcgg atccgacctg gggtatcccg gtcggctcta
     6301 gcccgcagtc gatggcgacg ttcgacgtgc tcggcgcgct tccggtcacg gtcgactcgt
     6361 gccgcttcga gcacctgcgg caggtcgtcg cgacgcgccg cttcgagcgc gtgaccacgc
     6421 tcgtgcggct cggcggcggc gggcacgagg ggatcggcga ggacgtatcc ctcaacgcgc
     6481 ccgaccacga cggcctcccc gacgcagacg cgctgccggt cggaggcggc ccccgaccgc
     6541 tgcgcgagtg gctcgcggcg ctgacgccgg acgccatgtg gtcgagcccg ccggcgaccg
     6601 acaccggcaa gcgctcgcgg cagtgggcgt tcgagagcgc cgcgctcgac ctcgcgctgc
     6661 gccaggcggg cctctcgctc gccgccgcgc tcgagcgcga gccgcggccg gtccgcttcg
     6721 tcgcctcctt ccggctcggc gaaccgccca cgctcgaccc cgtccggatc cggctcgacc
     6781 gctacccggc cctgcgcctg aagctcgacc cgacgaccga atgggacgag tcgatcttca
     6841 cgtcgctcgc cgatctcgac gtggtcgacg tggtcgacct caagggcgca tacaagggca
     6901 cgatggtcga ccagcccgcg gacccgcgga tgtacgagcg cgccctcgcc gcgttcccgc
     6961 gctcgttgat cgaggatccg gggctaaccc ccgagaccga gccgatcctc gcgccgcacc
     7021 gagagcgcgt ctcctgggac gcgccgatca tcggcgccga cgacgtgcgc gcgctggacg
     7081 ggcggccggc ggggatcaac gtgaagccct cgcggatcgg gtcgctggaa cggctctgcg
     7141 gcgtctacga ccactgccgg gccgaggcga tcccgatgta cggcggcggc atgttcgagc
     7201 tcggtccggg ccgccggcag atccaggcgc tcgccgcgct cttccacccc gacggcccga
     7261 acgacgtggc accgagcggc tacaacgagc ccgagccgcc gccgggcctg ccggtgagcc
     7321 cgctgccggt ggcgctcgac gcgccggggc tcggggcgga gccgccgccg gggggcgcct
     7381 tgcagtgaac ctggcggcga tcgtgacgcg cgcggcggag caggtgggcg agcgcgccgc
     7441 gatcaagctg ggcgatcggg tgacgaccta ccgcgggctc gacgaggcgt ccgcccgcgc
     7501 ggcggcgctg ctgcgggcgc gcggcgccgt gccgggcgac cgcgtcgtgc tggccctgcc
     7561 gaacagtccc tggttccccg cgttctacta cggcgcgctg cgcatgggcg cgatcgcggt
     7621 cccgctcaac gtgctgatgc gcgagcagga gctcgtcgcc tgccttacgc attgcgaggc
     7681 gcggctgctc gtcgcctggc acgaggcgct cgagcacgcc cgcgccgcgc gcgcctcggt
     7741 cgatttcgac ctgatcgagg tgaaccccgc cacggccgac gaactgctgg ccgcgcaccc
     7801 gcccgaacgg gcgctggccg acgtcgcccc ggacgccacc gcggtcctcc agtacagctc
     7861 gggcacgacc ggggagtcgc acgccgccga gctgacccac gcgaacctcg acgccagcag
     7921 ccgcctgatg ctcgaggcgc tcgggtccgt cgacggcgag gtcgtgctct cggcggcacc
     7981 gttcgcccac agctacggcc agaacgtcgg catgagcacg atcctccgcg gccacggcac
     8041 gctcgccctc atgacccgct tcgacgcggc ggaggcgctc gaggtcgtcg accgcgagcg
     8101 cgtgtcgata gccgtgggcg tcccgacgat gtgcgcgcgc atgctcgagc tcgacccgct
     8161 gccgagccgg atgccgtcgc tgcggctgtt cctcgcggcg gggggacgga tgccggtcag
     8221 gctgctgcac gagttcgagc gggcgttcgg atgcgaggtg ctcgagggct acgggatgag
     8281 cgaggcgccg cgcatcgccg tcaaccggcc cgggcggcgc aagccgggct cggtcggcct
     8341 gccattgcgc gagctcgagg tgaggctcct ggacgcgaac ggcgaaccgg ctccgcccgg
     8401 cgaggccggc gagctgtccg tgcgcggccc gaacgtgatg aagcgctact ggcggcgccc
     8461 ggcggcgact gccgagatga tgcgcgacgg ctggctccac accggggacc tcgccctgat
     8521 cgacgaagac ggctacatcc acatcgtcgg ccggctcaag gacacgatcg ttcgcggcgg
     8581 atacacgatc tacgcggcgg aggtggagcg cgtgatggcc acccacccgc gggtcgccga
     8641 ggtcgcggtg gtgcgcgtgc cggacgcgga gatgggcgag gaggtcggcg ccgcggtggt
     8701 cctcgtgcgc ggcggcgacc cgcccgaccc cgaggagctg aaggggttcg tgcgcgagcg
     8761 gctcgcgccc tacaagtacc cgcgcgtcct ctggttcgcc gagtcgctgc cgaggaccgc
     8821 gaccggcaag gtcatcaagc gggcgatcgc gatcccgccg gagctcgtcc gttcgggcga
     8881 gggagccggg ggaacatcgt gaggaacatc cgcgtgttca tcccggtccc gtccgggagc
     8941 gggacgtcct cccagttctc gtggcccgcg gggaggtcgc gatgcggcgc ccagtgcgcc
     9001 tggaagaccc gcaggacgcg catgtgcgtg tggttcaggt caccgaggcc gctgcgctcg
     9061 agctccgccg tcagctcgtc caccgtgagc gccgcgcgct cgctctgaac cgcctcgcgc
     9121 tcgagcacgg gcggcatggg cgacgtcgtg gagatgaagc cccggtacga catcggatgc
     9181 ttcgcgcgcc cgatgtcgaa gatccacacc gcgcagccgg tgcgctcgcg cagcgccgcg
     9241 atctggccga ggcaacggcg gatcaggtcg tcggtcggca ggtggtggag cgcctggatg
     9301 cagctcacga ccgccacgtc ctcgggcgcg gacgcggcga actcggtgat gtccccctgc
     9361 tcgagccgca cccggtccgc cagcccctcc tcggcgagca tgcggcgacc ggtggcgagc
     9421 atcggctccg agaggtcgag tccgacgatc ttcaggtccg gccggcgccg cgcgaggtgc
     9481 gccaggtagc gcccggaacc gctcccgagg tcgagcacgg tcgcgcccgc gggcgccagc
     9541 atgctcgtcg agcgggagca gagctggtag agcggaacct ggttgaccgg gcccgacgcg
     9601 tggaattgct cgaccgcctc gggatcttcc atcagcatcg tcggctcggg cacgcgcgga
     9661 tcgggcgctc gcctgaggac ctcgcgcgcg atccggtacg gcgcggccgg cagcgcctgg
     9721 agcttcacgc gctcaagtcc acagcccgct cccggccggg gcggagttcg ggagcacgcg
     9781 ctcgcgcagc ttgctgcgct gcaccttcag ggtggcggtg cgcggaatgt cgtcccacgg
     9841 caccacgatc ggctcggcga gcgccgggag gtcgcgcgtg gcctcggccc acgcgtcccc
     9901 gtcgagcggc tcgtcgcgtg ccgtgcagac gaccggcacc ggctccccgt ccttcacgcc
     9961 gagcacgacc gtctcggtga gggtcgggat ccggtcgagc agcacgtcct cgagcccgat
    10021 gcagctcgcg cccggtgtcc gatcgacctc gcggtcgacg agccggacgc ccccggtgcg
    10081 gcgcaccacg cccatgtcgc ccaggttcca ccacgtctcc ttgatcttgt gctcgtgacg
    10141 accgtgctcg ccgaggtagt cgacgcacgg ctcgcgtctg acctcgagca acccggcctg
    10201 cccgcggcgc agcggcttgc cggtctcggg gtcgaccgcg cgcaggcgcg acccgacgaa
    10261 cgagaccggg atgctgaact cctgcgtggc cggctcgcgc tcgccgtggc ggcgtaccga
    10321 gcggcgcgtg tgtagcgcga ccgcgaccgg cccgacctcg ctctgaccga gcgcctggaa
    10381 ccagaccggg aagcggtggc ccgaggcccc gaggaagcgc cgcacggtcc gcgcgtggat
    10441 cgcgtcgtag gtcgagaagt agagccgcac gttcttgaac ggccggctcg gctcgtcggt
    10501 catccactcc cagcgcagat acgcgttcgg cgaggtcgag acgatcgtcg gacggtgctc
    10561 gtcgagcacc ggcttcgcgc tgctctcgtc gagctcggac atcagcacga gcttcggccc
    10621 tgccgccgcg atcgcggcga ggccgccgag caggcgggcg tggaagtagg ggtcgcagat
    10681 cgcgaccgtg tcgtcgcggc gtgcgcgcag cagcgggatg cgcttgcgct cgtatcgggc
    10741 ggcgcggccg agcgtggtgg cggagtggac cacgagcttc ggcacgccgg tcgtgcccga
    10801 cgtgtggacg atcgccatcg gctcgtcgtc cccgcgcagg gcggggggcg gggtcggcgc
    10861 gccacggaac ggggcgagcg gcgtcacgtg ctcgccctcc tcgccctctc cgtcgacgac
    10921 gatcgtgcgc ttggtcagct cgcggagggt cgcgtcgtcg agcccggcgg actcgagccg
    10981 ggcgcggtcg gtgatcagca ccggccgctc gaggcggcgc agcagctcgt gcgcgacggc
    11041 gggcgggtgc gtccacgcga gctgggccgg gatcgcgccg atccgggcgc aggcgagcgt
    11101 gagggcgagg acgtccgggt ggttgccctt gaagatcgcc acgcggtccc acggctcgac
    11161 tccggcggcc ttgagccagc cggacaccgc ggcgatgaac tcggcgaagc gcgcgtagtc
    11221 gatcgacgtc ggaccctccg gatcgatgtc cggcggccgg tcgaagatca tcggccggtc
    11281 ggcgtggttc tcggcggcct cgtcgaggat cgtgccgatc cgtgcctcgg ccgcgtcccg
    11341 ggcctcgcgc cgtgcggctc ccagcagcac cgtcaggcac caccctcgga gcgacggaca
    11401 gcgggtgtga agcggttaac gagcagagca ccggtaatgt gccagatcga tcgggatgac
    11461 ggggggcgag gggataacga ttcgggactc aggccaggtg acggcacccg cctccgcgcc
    11521 cgcgcgcggc ttccgcgagc gcttcgtcgc gtacaaggac ctcgcgaagc tggagatgtt
    11581 ctatccgttc ttggcggtcc cactggtgtg gacgcttctc gagtcggagg tcgccaacga
    11641 ctcgaccacc ctggtgatcg tcgtgcttgc cgtcgtcgtc gtggtcggcg tgcgcacggc
    11701 ggagtcggcg ctcgacgacg tggccgggat ccgcgacggg atcgacacgt tcaactacga
    11761 gcagccgagc agcgacaggc gacgcacccg caagcccctg ctcgacaacc ggctcaccga
    11821 gcagcaggcg atcaactacg cgcgcatcgc gctgatcgcg tcgctcgcct cgttcgtcgc
    11881 cgcgggcatc atcgtcgact tcgacccgct ctgggcaccg atcgctgctg tcgtggcggc
    11941 gttcgcggtg gtcgcgtact cgtggggccc caagctcagc tacatcggtg cgcaggagct
    12001 gatcgcggtc ctcggcgtgg cggccgtcgt gtgcgtcacg tacgcgtacg cgaccggcgg
    12061 cctcacctgg tccgcggcga tcgagggact gctgctcggg ctgtggctcg atcagacggt
    12121 ggtgctcgcc aaccttcacg acctcgaggg cgaccggcgc gcggggcggg gcacgatggc
    12181 ggtgcggctc gcggcccgcc cgtaccagcg ctacgtgatt gcggtgttcg cgctggcgtg
    12241 gacgctgctg ctggcggccg tgatcctcgg cgagctgccg tggtggctgc tgctgctcca
    12301 gatcccggtc gtcgcgcttc aggtgcgcat cctggtcgtg gggatccgcc acgacgaccc
    12361 gctgtcgggc tggcggctgg ggcggcgcac gttcccgatc gcgtggatga cgctggtcgt
    12421 cgcgaacctg atcgcttttc actggtagcg ggcacccgcg ccaacggtgg ccgcctgctg
    12481 tttcgaggcg cgcgcagcgg gcagctagcg cgcatggaag cttcctccaa acagctcgcg
    12541 gtcgtcaccg gcgcctcgag cggcatcggc ctcgagctgg ccaagctgtt cgccgccaac
    12601 gacttcgacc tgatcgtggt cgccgaggat cccgacatcc aggtcgctgc gcagggcatc
    12661 cgcgactccg gcgcgggcgc gcgggccgtc caggtcgacc tcgccacccc ggcgggggtc
    12721 gaggagctgc acaaggagat ccaggccgag ggccggcccg tcgacgcgat cgcgctgaac
    12781 gccggcgtgg gcgccggcgg cgcgttcgcg accgacaccg acctggagga cgagctgcgc
    12841 ctgatcgacc tgaacgtgcg ctcgaccgtc cacctcgcga agtacgtggt gcgcgacatg
    12901 gtcgcccgcg gccagggccg cgtgctcgtc acgtcgtcga tcggcgcgac gatgcccggc
    12961 tcgttccacg ccgtatacaa cgcgtcgaag tcgttcgtgc agagcttcgc gctcgcgctg
    13021 cgtaacgagc tgaaggacac cggcgtgacg gtgaccacgc tcatgccggg ccccacggac
    13081 acggagttct tcgagcgggc cgacctcgag gacacgcccg tgggcagcgg cagccaggac
    13141 gacccggccg acgtggcgcg cggcggattc gacgcgctca tggccggcga ggaacgctcg
    13201 gtcccggggt cgctctcgac caagctccag ggacgcttca gccgcgtgct gccggacagc
    13261 gccaaggccg cgatgcaccg ccgcatggcg gagcccggct ccggcgacga gtagcgcccg
    13321 ccgtgccgcg ggcgcccgaa cctaggcggt aatcatccgc tccagggcga gcatctcggg
    13381 cgggtgccgg tcgaggtcct cgagcaccgc ctcgacctcg cacaccgacc gcgccaccag
    13441 cgtgatcgcc cccgcccggt cgagtgcctc gagttggacg cgttgcgtct gcgtcggccc
    13501 gggaccgcgc ggtgccttga cctcgacggc gatgaagcgt ccgccgtagc aggccaggat
    13561 gcgcggggtc cccttccgga cgctgctctc gccaccgacc tgaacgcacc aggcgccgcg
    13621 cctaagcagc atgctcccga tcgcgcccac aaggtcggcg acagcgttgc tgctcactca
    13681 gcctctcctc ccgttcagag acagaaagtg cgaaacctac atcggtgcgc aagtaggttc
    13741 ttttcgtggc agagcccgag ctcaccgacg ccgtatcgat cgtccttgcg gcgctcgccg
    13801 tggtgctcca ggcgctgctc gcgggactgc tcgccctggg tctggccgca ctcgcgtcgc
    13861 cgcgcgcccg gcgctggctg gcggagctgc gcgcgacgct cgccggcggc gagctgtggg
    13921 cggcctgggc cgtcgccctc gtggccacgc tcggaagcct gttcttctcc gaggtctccg
    13981 acttcgtgcc gtgccggctc tgctggttcc agcggatcgc gatgtacccg ctggcggtgg
    14041 tgctgctgct ggccgccgtg cgccgcgacg tgcgcggggg cgcgctctac gcgctgccgt
    14101 tcccggtcgc cggcgccctg atcgcgatct ggcacgtcta cgtggagatc aaccccgacg
    14161 ccgagagcga gggctgcagg gtggccgcgc cgtgctcgct caagtggatc gacgagctcg
    14221 gctacgtgac gctgccggtg ctgtcgctca cggcgttcgc cgcgatactc gccctcctcc
    14281 tgatggcgcg ctcgcgaccg gccgatgcct gacctgctgc tgccctacct cgcggtgatc
    14341 gcggtgctga cgatcacgcc cgggcccgac atgctgctcg tgctgcgcaa cggggtgcgc
    14401 gacgggaccg cggcggcgtg ggcgaccggc ctcggctgct gcaccggcat cgcggtgcac
    14461 gcggcgctgg ccgtgctcgg gctctcggcg atcctcgccg cctcgtccga ggcgttcacg
    14521 gtcgtgcggc ttgccggcgc ggcctatctc gtcgcgctcg gcgtgcaggc gatcgtgcgc
    14581 agccgcggcg ctcacgccgt ggcggaggaa cgcgcggagg agcccggctc cgcataccgc
    14641 cagggcctcc tgacgaacct gctcaacccg aagatcgcgc tgctgttcct gacgctgctg
    14701 ccgcagttcg tcgccgacgg cgagccgcgc gtggcgacga cggcggtgct cgccgcgatc
    14761 ttcctggccg gcgcgctggt ctggtggcgg gcgttctcgc tgctggtcgg cccgctaggc
    14821 cggttcctgt cccgcccgcg ggttgtcgcg tggttcgacc gcggcaccgg cgcgctgctc
    14881 gtcgcgatcg gcgtgcgcgt cgccctcgag cgccgttgac gcgtcggccc tgcgggtgcg
    14941 ctcgcgcacc cagatcagac cgccggccac ggcgagcgtc gcggccacgc ccccgcccac
    15001 gagcgcggcg ctcgctccgc cgccgccgcc ggacttcggc accggctccg ggtgcggctg
    15061 gccgaagtcg tgcgcgagct ggagggcgtt gccgatgagg tcgagcgcgg gcacgccggt
    15121 caggtcccgc tctcggagag cagccacagc gcgaggcagg tgaagcccac catcacgccg
    15181 agcatccagt actgcgagcg aacggccacg gacgcccgat cgtagagcgc cagcgcgcgg
    15241 tcgtgggcca gggccagcgc cgccacgtgg ccgagcacga cgaaggtgac ctggaggtac
    15301 cagatcgtct cgctcgcgac ccagttggaa tcgacggtcc agccggcgaa cccgaagagg
    15361 tccgagccgc tgccgagcgg gtccgacagc agcgccggga cggcctgccc ctggatcagc
    15421 aacaggctca cgtagtgggc gagcacgtac gcgactccga tcgggacgag ggagtgcacg
    15481 aatgcgcgcg ccaggcgctc gcgcgagagg tcgccgccgg ccgtgcgcac gccgacgatg
    15541 ccgagccggt agaagccgag cacgagcccg atcacgacga acaggccgac cgccgccgcg
    15601 aactgcccgg ccgtggctcc gctcatgccg agcgagctga acgcgtcgat cagctccggc
    15661 tcgaggtcct gtcgccacac gcccttggtg gtcagcccgt cgaacgtggt gctcccgatc
    15721 atcaccgcga gcagcgggac cgtcccggcg agcgggagga cgccgcccag gcccgacagc
    15781 agccggcggc ggccgaccgc gtcgccgcgc cgctcgaaca ccgacagacg cgcgaacagg
    15841 ccgaagtaga ccgagaacgc ctcgccgcgg tcgagccagc actcgatccc gtacaccgac
    15901 atcgcgaacc aggtggcggc gctgtacacg agcgccgcga ccgccagcga ccgcggctcg
    15961 ttccccgccg tgaccaccag ctccatgaag aggaaggcca gcagccccgc ggcggcgggc
    16021 cagtggccga ggcgatccgg gtagtcgaac ggctccgacg gctcgcgccc cgcgcggcgg
    16081 aacgcccagc tcgccgcgcg cgcgaccgcg cgccacgggt tgaacgcgcg gaacacgtcg
    16141 ccgaacacga cgctcaccgg aaccagcgcg acccagaaga ccacgaagac ccacgtgggg
    16201 gcgaggttgt cggtctcctc gcccgccagg ccgctgtaga cgaccagcgc gagcagcagg
    16261 gctccgatcg cgccgcacgc gacctccacc ggacggctcg cgagcagccg gccggcgcgg
    16321 ccgagcgggc gccactcacc gtcctcgagc cgcggcttgg gacacagcac ggccagggcc
    16381 acgaacgaga tcagcaggac cagcgcggcg ccccagacga acagccaggt cgggaccggc
    16441 aggtcgcgct tgacctggat cccgtgggcc agcgccggct gcgggacggc ggccaggatg
    16501 acggcggccg cggccaccgg cagcgcggcg cgccgcgcgg tcacggctgt accttcagct
    16561 ccgcgatctg cgtcgcggtg tcctcgacct cgatctcgaa gatgccgctc tcgtttgcct
    16621 tgaactggaa ctcggccgga cgatcggccg tggtctcctc gctgatgtcg tagccgtgca
    16681 ggtggatctc ctgcggcgcc tcggtctgca cgcggaagcg gaccgtgtcg ccctcgctcg
    16741 cctcgatcgt ctgaacgcct cccaccggcc gccccccgcg cacgcggatc agcttcaccg
    16801 ccggctcctt cggctgcgcc tcgggggccg gctgggtctc cgccggggtc gtggtggtgg
    16861 tcgtcggcgc gggctgggcg gtcggggtgc cgcgcttcga gtcgtcgtcg tcatcgccgc
    16921 tcgggcgcag gacgaggaac gccgcaacgg cgaggaccgc tgtgacgatg aggatcgtgg
    16981 cgcgccggcc gtccatgcgg cgggcagtat gccaagcgct ctttatgcgt tcgattaacg
    17041 cgcctcgcgc cgcctggtcc acgagcccgg cgcggttcgc gagccgggtt tttgggtaat
    17101 tcccagtacc cctacaggaa ggaccctcgc atgattggtt acctgatcgc cctcgccctg
    17161 acgggcctca tcgtcggcgc cctcgcgcgg ctcgcgctgc ccggcaagga cccgatgtcc
    17221 atccccatga ccatgctcgt cggcatcgcc ggctcgctga tcgccgggct cgtgtactac
    17281 gcgatcaccg acgaccgtgg cggtctcggc atcctgccgg ccgtgctcgt gtcgacgggc
    17341 atcgtctacc tgatccggcg ctcgcgcggc ggcaccctga cgcaggccgc tccgcgcgac
    17401 ggcgtcggtg gccggcagcg cccgctgacc cgctagcagc cggcaccgag acggcgcgga
    17461 cgcgaggcgg gtgccgcgtc cgctctaggc ggcggcggcg cgtgcgggga cttccccggt
    17521 gcgcgccgcc cgctgctcgc tgatcctgcg cagccgccgc agcgtctcca cgttgcgcgc
    17581 ccacagcgcg cggtcgatca gcgggttgtt caccttggcg accggaccgc cgatcggata
    17641 ctcgatcatc gtcacgtgag tgccgcgccc gcgcgggcgc agccggaact cgaccttccc
    17701 gatcatgaac ggacggaagc ggacgacgat cacgagctgg cgcggccggt tcgccgcgac
    17761 cgccgtggtc gagtccttca ggccgatgcc gaaggcgccc tgggtgtgat ggaacttggt
    17821 cccggcctcg ggccaatcgc cctcgacgtc gcggatctgg ctggcgccca cgacccaatc
    17881 cgcgtagctg ggggcatcgg cgagcacatc gaagacggcg tccggcgggg cgtcgatgta
    17941 ggcgtggttc tcagccatcg cactgagagt acggagagcc gcgccgtgcg gatcaggggc
    18001 gcggcaggcg cgtcgagtcg acgacctcga agttgcttcc ctcgacgccc ttgagcgcgt
    18061 tcagctcgtc gttgtcccag cccttcgagg gcgcgcccga gatgaaccag tcggacccgt
    18121 tgtcggccac gatcatcccg tagcgcttga gcgcgttcag gatcacgcga gcctgccgcg
    18181 ggtagccgga ggtgtcgaag ctcgccttca atcgcagccg ctggcccatc gccggcaggt
    18241 ccgggtcggt gctgctcgcg gcgaagtggc gggccgggta gacgtacgcg cggcggctgc
    18301 gggagacggt gaagcgcagc gcgtggtcga tcttcccgcg cttgacgtcc tcgtagcgcg
    18361 cgaggcccgg gaggatcgga agcccggccg catcggccga ggtccagccc gcggggcgga
    18421 cccggttcga gcgcaggtcg aagatcgcgc cggagccggc gcgccacgag cgcccgccgt
    18481 cgcgcgggta cgccgcgtac agctcgtaga gcttgcagcg gtcgcggtcg acgacgatca
    18541 cgtggcggtc gccgtcggcg ctcgagccgc cctcgatcgg ggccttgggc gggatcgggt
    18601 aggtgccgcg gtcggactcg tcggcgtact cgaagctcac cttcacgcgc ttctggctct
    18661 tcgagacggt cgtgtacggg atgccgatcg gccggccctc gtagagcccg gagccgaagt
    18721 cgggatggag ggtgccgtcg gctccgatcc cgcgcaccat cgcgtcggag ttcggcagca
    18781 cggggagctt gtccacgcgc atgttccagt ggttgtcggc cgggaggatc cggcacttcg
    18841 gcgcgccctt gaccgggccg ccggaggcgg gcaccgcaat gacgagggcg gcggcggcga
    18901 ggctcagcgc gggcagcagt cggcgcatct gaccaggcta tcggcggtat gctcgctttc
    18961 atgcctgagc gcgaggaatc ggaccagctt cccgaggagg gtcagggggg cgaggtgcac
    19021 gacgacgacg gaggtcaggg cgcctcgcgt gaggatgcgg aggaccaggc cggcgccgcc
    19081 gacgatgacg gcgacgaggg cgagggccag gccaccggga acccgcgcag cgccggctag
    19141 ccgcgtaccg ctcgcgagcc ggctcgaccc gagcgccgcg cgcgaggcgt ggcgcgcgtt
    19201 cgtcgaatcc cgggtcgttg tgttggtcgc cgcggtgctg ggcgtcgcgc tctttccgct
    19261 cagcgggcgt gcccaggcgg gccacccgga catagtccac ccgttcgagg gctggccgct
    19321 gggcgagctg ctcgactctc tcgtcgcgcc tttgattcgc ggcgacgcga gctggtacat
    19381 gatgatcgcc cacgacggct acgtcgacga gggcatccag accggcgggc tggtgcaggg
    19441 gcggccggcg ttcttcccgc tctacccgct cctgatgagg gcgctcggcg ggttcggcag
    19501 cttcgggttc gcggcgctcg cgggcacgct gatctcactg gcgtcgctcc tcggcgcgct
    19561 gtatctgctg taccggctca ccgagctcga gctcgggccg ggtgccgcgc gggccacggt
    19621 gcggctgatc gcgttcgccc cggtggcgta cttcttcagc gcgccgtaca ccgagagtct
    19681 gttcctgctg ctgtcggtcg cctcgatcta cgcggcgcgg cgcggcggct gggctgcggc
    19741 gggactgctc gccgcggcgg cgtcgggtac gcgcaacgtg ggggcgctgc tgatcgtgcc
    19801 gctcgcgctc atgtacctgc gcgagcacgg gcgcccgggt ccgcggatgg cctgggttgg
    19861 gctggcgccc gcggggctcg tcgcgttcag cgtctacctg cgcgcggacg tgggcgactg
    19921 gcaggcatgg cggcacgcgc aggtctactt cggccggccc gagaccgtga acccgttcga
    19981 gggcgcgcgc cagggcgtgg ccgcggcgtg ggatgccctc gcgggcagcg cgcccgacga
    20041 cctccagatc ccgatgctgg tcgcgttcgg gttcctcgtg ttcgcgctcg tcgcgctggt
    20101 cggcgtgttc cgcagcctgc cgctcgccta cggtgcgtat tcgctcgcgc tggtgatccc
    20161 ggcactctgc ctgcccgtgc tcgacaaccc gctctacggg ttcccgcgct acacgctcgt
    20221 cgcgttcccg ctgttcatgt ggctcgggct gcgctgcgag cgggcggggg tgaccgatcg
    20281 ggtggtgctc gcgttcgcct gcctgctcgc ggtgttcacc gccgggtacg cgacctggca
    20341 gccgatcggc tagacgccga gccgttcgcg caaccagagc ccgttgcgca ccgcgaagcc
    20401 ctcccagtcg ttgttgaagt aggcgaagac ctccaccctg cggcgccact cggcgatccg
    20461 ctcggcccac tccgcgagct cggactccga gtagttcccg cgccggccgc ggtggccgta
    20521 gtggaagcgc acgaaggtcc agtcggcggt catctcgtag ctctggaacg gacgctcggg
    20581 gtgatcgccg atcgtcagtg ccacgccgtg ctcgcgcagc agctcgtaca cctcgggcac
    20641 gaaccagctc gtgtggcgga actcgaacgt gtggcggccg gacggcagcg ccttcagcgc
    20701 ggcggccagg cgatcgtcgt cgcggtggaa gttctccggg agctgccaga ggaccggccc
    20761 cattttcggc gtgcgggtga gcggctccac gcgttcgtag aacttgtcga tgccggtgtc
    20821 gagcgacgtg agccgcttga tgtgcgtgat gtagcggctc atcttcaggg tgaagacgaa
    20881 gtccggcggg gtctgctcga cccaccgcgc gacggcgtca cgtgaggcaa gccggtagaa
    20941 ggtcgagttg acctcgaccg tgtcgaacag cgtggcgtag tgctcgagcc atcggctcgg
    21001 cgggcagccc ttcgggtaga ccagctcacg ccagctctgg tagttccagc ccgagcaacc
    21061 gacgcgtacg ggcttcacga tcgcgccggg cgccgtggct cagacgacgg tggtcttgtc
    21121 ccgggcgtcg aagtcgaccg gctgcgcgcg cgcctcgcgg cggaagcgct tcatccgcat
    21181 gatgcggttc tggcgctcca tctgctcctt gccgatgacc gcgccgatga agatcagcgc
    21241 gagcgggatg cccacgtatg gcgcgccgaa ggcgagcgag ccgacaagcc cgccgaggat
    21301 gatgaggccg aggatcccga gcccgatgct catctagcgc ccctgctcgt cggtcgtctc
    21361 gaatgtctcg ggcgcgtcgc cggcgccctc gaccaccggg cgcatctcct cgggcgcgtc
    21421 cgcggtgacc gtgtcggcgt cgccgccgat cgagccggcg tcggccgccg cggcggcctc
    21481 ctgctcgcgc acgagctggt cgtcgttcga gatgtccggg atcacggcct gctcgtcgtc
    21541 gccgatgccc gcgatcgggg cctcgatggt ctcctcggtc tgggcggggc ggacctcggc
    21601 gaggtcctcc gcggtcgtgt cgggggcctg ctgctcgttg acctggacgt ccggcgcctg
    21661 cgcgctgctg gtggacggca tcggcgcgga ctgctgcgcc tcctggccgc caccgccgct
    21721 gcgcttcttg aggaggaagg cggtggctcc gccgagtagc gcgagaaggg agagcttgcg
    21781 gaacatggtc tgtttctcca gggagtcgaa gggggggctg gtcggcgtct acccgaagat
    21841 acggggagcg aaccggtcgg cagcgtagcc tgaacgtcgt gctggagacc ctcaagccga
    21901 tactccgcgc ggccgcgata gcgggcttca ccgtcgggct gatcctgttc ctggtgaacc
    21961 cgccgtcgct gtcgggcatc caggggctgc tcgtgtggac ggcgggcgcg gtcgccatga
    22021 tcctgatggg cgcttgggcg gcggtcgcgc tgaccgggaa cgagtcgatg accgaggagg
    22081 aggtcgagcg gctcgccgtg cgggcggagg atctggcggg gcggggggct ggtgggggcg
    22141 ggggtgccgg ctcggcttcg gccgggggcg acggcgagcg cgggccgacc gacttcgaca
    22201 cgctggtcgc cgacgcgatc gaccgcctgc cgccggagtt cctcgacctg ctggcgcgcg
    22261 tgccggtgat cgtctcccac cgcgggcgcg agttccgcgc ctacggccac cactacggcg
    22321 ggccgtacac ccgcggcgac tacgagcacc ggatcgtgat ctaccaggac acgctggagc
    22381 gcgacttcgg ccacgacccc gagctgctgg ccgcgcaggt ggagcggacg ctgcgccacg
    22441 aggtggcgca ccacatcggc tgggacgagc cgggcgtgcg ggggctgggg ctctgagcgt
    22501 gccgcggccg ccggggccac gggcggccgc cggcgtcctc gcggcgcggc cggccaccga
    22561 ttggagcctt actccgccat acggtccata accctccaat cgatgcgcac ggctagcctt
    22621 cgcgattcct ggagaggtgt ccgagcggct taaggagcgc gactggaaat cgcgtgggcg
    22681 gggaaacctg cctcgagggt tcgaatccct ccctctccgc tgaagcagcg agcatcggcc
    22741 tcgtttgaac ctggggcgcg aaggctgacc agcacgtccg ccttccatcc gcgcggagac
    22801 atctcctcga tccggcccag tgcccgccag agcggcgcgc cgcccgcgcg cggggtgtcg
    22861 tagtgtcgcc gggtcgtgat tccttccgag ccggaggccg agtcgtctcg acagcagcgc
    22921 tggcttccgt ggcgctggcc gcgcggcgga atgcaggttc tcgcggaggg ggaggcgcaa
    22981 gtgctgagcg atcgccttcg agagaaggtg cgtatcagct ttccgcagga tcccgggcgt
    23041 gaggagaggc cagaggcgtt cgacgactgg ttgcgtctcc gcttccaagt gcccttagag
    23101 cgagatgcgt tggcagggcc tgcgttcggc aagtcagcca ctgcgcttgg agtcgcgacg
    23161 attggcgcgg gcctcgcttc ctcggcgatt acgacggcct ccggcgggga ctgggcaacg
    23221 gtcgtcgtgg cttcgcttgg cattattgtc ggcgtcctag cgacgatcaa tcagatatgg
    23281 cggccctcgg agcgatctgt cgcgcgatac caggcggcgt tcgcgcttcg tcgggagggc
    23341 tgggcctacc tccaggaccg cggccggtac gagcgggtgg ctccggagga caggctcccg
    23401 gcgttcatcg acgaagtgac gcgcatccac cgcgcggtgg agctggttga cgaggcgacc
    23461 tctgcgagtc agaccggcgg ttgattcggc tgatgcgggc ccgacggtgc aggccctcga
    23521 ggaacacacc gcagccggcg gtgcagccgt tcgatccgcg cgcggactac ttggttcaaa
    23581 taccgtccct cccaccccgc tgaagcaggc gagcatgtcg cttatgcgaa cccgtgtcgc
    23641 ggcgggtgcg ctcgaaccga gagccgcggc tacctcatga gcagccgctt cgtctattcg
    23701 gctgagggca ctttgccggt ggaagacgca cgactgcctg cgtcagcgct gcgcctcctg
    23761 gaactacgtt gaggtcagtt caacctcgtg gaggtttctg ctccaggcgc agccctggat
    23821 cgtgctcgcg caatcgggcg gtgagccacg ggcgatcaca tctgtggcgg taggtggcgc
    23881 ataagccgtt cggtctgatg gcaagctccg ccactgggtc ctcatccggc cgggacgcag
    23941 ctttgatatc aggaatgcgc gggcctccac cggcatcgtc ttgggtcgcg acgaccgtcc
    24001 cgtcttcgta gaccacggct ttgccgctgc tgccctccgt ccacggacgg aggacgatct
    24061 cgcgctcggg ggcgtctctg ctgcgctcgc ggttctctcg caacacttcg tcagcgagcc
    24121 gcaacgcctc gtcacacgtg cccgtgcgcg cgaagggttg ctcggtgaag gggttcggga
    24181 tcgggggcca gtggtcgccc ggccggccga cctcttcgaa tagggcgaag aagagcctgt
    24241 agccccaggc ttctcctcga ccgatcaggt cggccgcctc acgcacctgg cgcgaccagc
    24301 ggagcttcgg gtcgtcgccg tgatcgtcga tctcgtcggc cagctggtag agcacctgct
    24361 ccagccgctg ctcggttgac atagcctcgg gctgcggctc caccggctgc tcgtctgaca
    24421 cagcctcggg ctgcggctga tcactgcgcc gcttacgacg ccataggctc attcgcggtc
    24481 cggttcccgg gccaaagcgt tccacatggt cgcgagcttg tcaggaaggc cctgcccgcg
    24541 ggcgcagccc gcaccgccgc accgctgcct cgcatggaaa gcagcagtcc ggcgcgctca
    24601 cacgacgacg ctgctggcag gcgaaagcaa cactcaccag tcgagcgtca gactgcggca
    24661 tagatgatgg agcacccggc ctcagaggcc ctacgggcac aatcgccgca gctgctgcat
    24721 gacgccctgg tcgcggacct ccgcaacggg gagtccgagt ggctgacgga cccacgcgac
    24781 ctcatggtcg cccttgcgcc gtttcacgac tgcgctgcga ggctcgggct caacgtgagc
    24841 gatgcgttcc ggcgcgcagc agacgacggg ccgagaagcc tccgcgacct cgtggcgacg
    24901 tttggcgcac ggggtgacgt gacgccagat gcattcggct tcgtgctgac cgagggtccg
    24961 gatgggctgt cgtaccgctg tcgctgaagc tgctgctttg aggcaggagc caccgcgctg
    25021 gcacgcggtc ggcggactgt cgcaacggac gagacgcaga agcggcgagc aggcgtactt
    25081 gcgctgctgc ttttcgatca aaccagcagt cgccggtcgc gcgggcggcc tgcgcgtgca
    25141 atttgcgatg gagtcgcccg attcgctcgc gggcctcggg gttcgcaaac cgtctcaccc
    25201 agtccgctga agcaggcgag catgtcgctt gtgcgaaccc gtggcggacg ggggcgagag
    25261 caggccactc acgtaccctt cacgcctcga tcaaggaacg caaattgaag cgcaaagtca
    25321 agttcagccg cgacggcttg acccccgtgg gcagcctctt cgctcccacg ggcttcgatg
    25381 agaacgagca ctacaaggca gtgacgtcga ggctcgttca catcggtcaa ggagcagatg
    25441 ccggatacct acgcccagaa gttcgcggac cagggctctg tggccctcgc cttcgactac
    25501 agccactacg gcgagagcgc aggggaacct cggcagcttg agttgccggc cgagaagctg
    25561 agcgatctgg aggccgctgt gagcgtgccc gcgcgggagc gggccctgcc gccggccggc
    25621 gaggtggtgc tggacgttcc cgcccttgcc gccgatcagg ccgcgcgcgg gacgacggtc
    25681 gtcgggccgc ccatgggcgc cggcgattag gcagcaaggg cgggagcggc cgggcgcggg
    25741 agacgccagg cggctcccgt cagccgtcct gctcgccgag caccacccag gcggtcgacg
    25801 gcgtgtgaag ctcgggccgg tagccggcga cgtgcgccgg gacggcggct gtcgtcagag
    25861 cgtcgacgag catctcgacg atcacgcgac ccatcggcga cgtcatcgat cccttctgtc
    25921 gagggagacg cgcgcgccgt gctcggacca ctcggcgtcg aagacgtggt tcggggtgta
    25981 gacctcctcg tgctccgtcc cctctcgttc ctcccaggac atgacggtgc cccgttcgtc
    26041 gacgtggagg aggcgcggca gcaccggacc gagtgcggcg gcggcgcgct gcgcgtgtgg
    26101 cgtgatgacc gtgaacgtgc ccgttcccgg gtgctcgtcg aactcagggc tctcgccatc
    26161 gaggcggagt ctgggcccga gcacgtccgg cgccacgctg atcagcgcgc gcgccggggc
    26221 atcggggtag cgcctctcgg cgtcctgcag cagccaccgt gcggtggcgg cgtcgccctc
    26281 gcgaaccgcg aggcgcacga gcgcgtgcac ggcatcgagc gaccctgacg ggcactctgc
    26341 tcgtaccact gccgcgcctc ggctcgggct cgctctgcgg tggcctccaa ccgcgccgcc
    26401 agctcgaaca tcgccgaggc gtcctgctcc tccgacgcgc ggcgccgcag ctcgtttcgc
    26461 tcgagtcgtc tacggcgtgt cgtccagcgt cgccgacccg cgcagcggat cgtggtcgga
    26521 gtagctcgag gacgtggcgt cgccgtcgac ggcatagaag tcgcgcgcgc tcacgaagat
    26581 gtagtcgatc ttccggtccc cgtgagtcgc ctcgccgcag cggcatcggg tggtgctgcc
    26641 gttgtactcg tcgacctcct cgaaccggcc ggtcgcgccg ccgcccaaca ccgagtggta
    26701 gatcggatcc agccggtcgt cgttcggctc cacgttgaag tcgcccatca gcacgacggg
    26761 ggtcccgctg tcgacccact ggttcaccgt gctggcgacc ctgtcgatct gggccttgat
    26821 gtccgcgtcg taggacgtga tgtgaaccga gcagtacacg gtgtcgcggg agaggtccac
    26881 gaccatgcac cacagcttgc gtgtctccgc cgtgctctgt gtcttcggca gcgacgtggc
    26941 cgacggctcc atgaggatcg tctgcctgct gaacagggcg ttgccgaagc gccggtcgtt
    27001 gtcgtagagg ttgcagttga tcgtcgacgt cgaggtgggc gcgctcaggt accgcgtcgt
    27061 gacgaagcgc gaacccatgc tccacaagcc ggcggcgttg aggttccagt aggtggtcgc
    27121 ccactgggac tggcacgcct cgttgaggct gaccccgtcg ggcttccagt tctggatcga
    27181 gttcttgatc gcgtccgcca ccggctgctc ggcgccaccg ttgcacggag ccttgatcac
    27241 cgccccgcac atgttgaagt gccagaagcg cagcggcgtg tcggctgaag ccgacgctcc
    27301 ggtgcagagg aggaaggcgc ccgcgagggc gcagatcgtg acggaccggc gtaggagacc
    27361 catgtgcaac gcctacccgg atcgcgccgc cgctagccgc ctgcacggta taaggaaatg
    27421 gcgcgttggc gccgaagtag atcctgatgg cgaccgatca aaccgtcctc gcgaagaccg
    27481 gccgggtcac cggccgcgtc gcccccggca ccgtcggcga agtcgtgctt ccgatccgcg
    27541 gaggctcgga ggccttctac gcctacgccg ccgagccgga tcaggtgatc gagcccggta
    27601 cccgagtcgt cgtcatcgac ttcgaggagc cgcgcaccgt gcgcgtcctg cccgtctaag
    27661 gagaaccacg aatgagcgtc ctgatcacca tcggcctgat agcggcggtg cctctcgtcc
    27721 tggcggccgt cgtcgccctg atcgtcaagc tcacctggcg cgtcgcggaa ccgaacgagg
    27781 ccctgatcat ctccgggctc ggcgcgagct cgcggcaggc gctcgactcc ggtctcggct
    27841 tcaagatcat cgtcggcaag ggcacgcttt cgctccccgg cctccagacc gtccgccgac
    27901 tcgggctcga gctccacaag gccgacctca ccgtcgactg cgtgaccacg cagggcatcc
    27961 ccgtgcacgt ccgcggcgtc gtcgtctaca aggtcggcga cggcaccgcc gagatcgcga
    28021 acgccgcgcg gcgcttcctc gagaaggagc acgagatgga cgcgaacgtg cacgaggtgt
    28081 tcgcgggtca cctgcgccag atcgtcggcg cgatgacggt ggaggacatg atccgcgccc
    28141 gcgagaagct cgcggccgag acgcgcagct cgtccgccat cgagatggag aagctcgggc
    28201 tcgtgatcga ctccctccag atccagcagg tcgaggaccc gaccggctac atcgagaacc
    28261 tcgcgaagcc gttccaggcg gccgtcgagg ccgacgcccg gatcgccgct gctgagcgca
    28321 accgtgaggc gaccgagcgc gagatggcgg cggccgccgt ggcggccaag gcggtctcgg
    28381 actccgagat cgagcaggcg cgcctgcgtg cggcggccaa cacggcgacc gcgcaggccg
    28441 accaggccgg accgctcgcg gaggccgagt cgaggaagag cgtggtcgtg caggagacgg
    28501 aggtcgccga gctcgaggcc gcccggaccg agaagcggct tcagaccgac gtggtcaagc
    28561 ccgccgaggc cgatcgcgac gcgcgcatcg cccaggccga ggccgagaag cgcgaggtgg
    28621 agctcgccgc cgcggcccac gccgagaagg tgaagatcga ggcggccgcc aacgcggagc
    28681 aggtcaagct gaaggcgggc gccgacgccg agcagacgcg cctgaacgcc gacgcgaacg
    28741 cgatcgcgac cgagcgcacc ggagcggcgg aggccaaggc gacgacgctc aagggcgagg
    28801 ccgatgccag cgccgtcaag gccaagggcc tcgccgaggc cgaggcgatc gacgcccgcg
    28861 cccaggcgct cgagcgcaac caggacgcag tcatcggcca acaggtcgcc gagcagctcc
    28921 ccgagatcgt cagggccgcg tcggagtcgt tccgcggcat cgacaacctg acggtgctca
    28981 acggcgccgc cggcatgaac gacatgatga cccagatcat gagcatgggc gcgtcggcga
    29041 ttccgatgtt ccgctcgatg ctggccggca actccgccgc acccgacgac aacggcgccg
    29101 gcacggccgc gctcggcacc tcgaagggcg acgacaagac ctgagccggg cgggggagta
    29161 gcctcgcggg atggaccagc gaacacttgc cggtaccggc gtccacgtga gcccgctctg
    29221 cctcggcgcg atgatgttcg gcgcctgggg ggagaccgac cacgacgccg gcatacaggt
    29281 catccaccgg gcgctcgacg ccggcatcaa cttcatcgac accgccgacg tctactcgcg
    29341 cggcgaatcg gaggagatcg tcggcaaggc gctcgccggc ggtcgccgcg acaacgtggt
    29401 actcgcgacc aagttccacg gcacgatggg tgacgacccc aacgaggcgg gcaactcgcg
    29461 ccgctggatc ttccgcgagg tcgaggcctc gctcaagcgg ctccagaccg actggatcga
    29521 cctctaccag gtgcaccgct gggatccctg gaccgaccac gacgagacgc tgggcgcgct
    29581 ctcggacctc gtggcgcagg gcaaggtgcg ctacatcggc tcctccacct acccggccgc
    29641 gcagatcgtc agggcccagt gggtcgcgcg cgagcgcggg taccagcgct tcgtctgcga
    29701 gcagccgccg tactccctgc tcgttcgcgg gatcgaggcc gacgtgctgc ccacctgcct
    29761 cgagcaccgg atggggacga tcgtgtggag tccgctcgcc ggcggctggc tgtcgggccg
    29821 ctggcgcaag ggcgccgacg acctgacctc gcggcgctcg gcgatgatcc cgcagcgcta
    29881 cgacctctcg attcccgcga accaggccaa gctcgacgcc gccgacgcgc tcggccggct
    29941 cgccgacgag gcggggatca cgctggtcca catggcgatc gcgttcgcga tccgccaccc
    30001 gggggtgacc gcggcgatca tcggcccgcg gacgatggag cagctcgagt cgcagctcgg
    30061 cgcggtcgac gtgcagctcg acgacgagct gctcgaccgg atcgacgcga tcgtcgcgcc
    30121 ggggacgacg atcaacccgg ccgacgccgg gtggacgaac cccgcgctcg ccgcggaggc
    30181 ccgccgccgc tgagcgccgc cgacgcggcg caggcgcagt cgcgctaaat tccgttcgca
    30241 ccgctggggg acccgtcgcg ggggcgggct gcggtcaagg gggcaagcat ggggcgcagg
    30301 gcacggttcg gtttcgcgac ggcgattgtc tgcgcggcta tcgcggcagc tccggcggcg
    30361 gcgggggcgg cgaccccggc tccgacaccg gtcaccggca cctcgtcggc gttcgccgac
    30421 tcgatcggca tcaacgcggc cgacttctat cccgacacgc tctacgcgaa ccacgcgctg
    30481 gtcaagcagc ttctgctcga cctcggcatc aggcacctgc gtggcgggat tcgccttggt
    30541 cgcagcgcgc aggagtacgc ggacgtccgc gacctggccg cggccggcct gaagaagatg
    30601 tggatcaccg gccggcccga ctcgacccag ggcgacccga ccaacttcag ccaggtccgc
    30661 gacatcctca cggacccgaa ccggctcggc ggcacgaccg aggcgctcga gggtcccaac
    30721 gagttcgact cgagggcctc cgaggactcg ctctgggcgg acaagctcca cgtgttccag
    30781 acccagacct acacgtgggc gaactcggac tcgaccttct cgaacctacc gttctacggc
    30841 ccgagcttcc tcagccagag cggtcgccag acgtacgcgt ccaagccggg cgcgaacaac
    30901 gtgatggacg cgccgaacgc acacccatac gcgggcgggc ggatgcccga ggaccggatc
    30961 gcggccgaga tctcggagct ggcgggcctg ttcggcaacc gcaagcccgt catctcggag
    31021 accgggtacc acaccgcgct gaacggcggc aactggtggc actacggcgt ctccgagcgc
    31081 gcgcagtcga tctacctgat gcgcaccctg ctcaccgcgt tcgcgctcgg cgccccgcgc
    31141 acctacatct accacctgct cgacctcaag ccgaatccgg cgcaggacga cctcgagatg
    31201 aacttcggca tcgtcgccac cgagggcgac ccggcccagt ctgcgcagcc gacctggacg
    31261 gcccggccga agcaggcata caactcgctc aaggaggtct tcgacctcac cgcgagcagc
    31321 ggcagcgtcg acgcgggtcc gaccaggctc agctacgcgc tgaccggcgc gccctcgacc
    31381 ctgcgcaagg tgatcctctc gcgccgcgac gggtcgaccg acctcgtcct ctggaacgcg
    31441 gtccccgtgt acaacgaccg cagctacgac tgcaacgacc cgcgcgtcca gcagtacccg
    31501 gcccaggacc gctgcttctg ggcgatgctc ctctactccc aggaccaggg ggacgcgttc
    31561 ccggccgacg tgcccgtcac gctcgacctc gatgacactg cgcgcgtgag caccgagcgc
    31621 ccacacagcc agacgagctt caccgcgctg ggcgagggta agacgttcaa cctgaatgtg
    31681 ggggccgacc cggtgttcgt ccggatcgag ccgcgctacg gaatggcggt gcgggccgac
    31741 cagccggcgg cgtggctgcg gctcggcgag tcgtcaggcg cgccggggga ctccgccgga
    31801 cgcggggcca ccacgaccgg gtggacctcg gcgcccacgt acggccgccc cggcgccgtc
    31861 ggggacgcga gcacggcggt cggagtgagt gggagccagc gcgcgacggt gaacctcgcg
    31921 agcccggtgt cgacggacac cggcacgacc gtcgagatgt gggtcaggcc cgacacgacg
    31981 acgccggact tcaccgagtt cctcacctcg aactcacccg acgtccgcgt gcgcatgcgc
    32041 gcgtacaacc ggcacgacag ccgcgactgg ggatcgacct acggctcgcg gccaggcgcc
    32101 accggcgaga tgatcttccc cggcttcgcc tacggccagt ggcaccacgt cgtgctcacg
    32161 atggcggcgg gcggctcggt cacgtacctc gatggcaaga aggtcggcga gaccaccggt
    32221 ggcgaggcgc tcaacgtgag cgcgttcacc gtcggggcga acggcgggaa cggagggttc
    32281 aagggcgacg tcgacgagct cgcggtctat ccggccgcgc tgagcgcagg ccgcgtctgc
    32341 gcgcactaca gggcagcggg cgggagctgc tgaggctcga ctggcccgcg gccgaagacg
    32401 cgcctcgctc gcatgccgcg ctgacgctcg ccggccgatc tgatagccgt tagggccgaa
    32461 tgtcctccgt cgaccgactc ctcgagaaca gccgcgcgta cgcggccggg ttcgcgaagg
    32521 gcgacctcgc cgcgccgccc gcgaagcggg tggcggtgct cgcatgcatg gacgcgcggc
    32581 tcgaccccgc gcgcctgctc ggcctcgagg agggcgacgc gcacgtggtc cgcaacgccg
    32641 gcggtgccgt gaccgacgac gcgatccggt cgctggcgat ctcgcagcac ctgctcggta
    32701 ccgaggagat cgtgctgatc cagcacaccc actgcgggat gcagggggcc tcggaggacg
    32761 agatggccga ggctctcgaa cgcgcctcgg gtgagcgccc gccgtggccg ctgcacgcct
    32821 tcgaggacct ggagacgaac ctgcgcgagt cgctgcgcac tctccgggag agcccgttca
    32881 tcctggacag cgcgacggtg cgcgggttcg tctacgacgt gaagacgggc gagctgcgcg
    32941 aggtgcagta gaggccgtcg cgcgcggctc ggcgctccgc ccgcggcggt aggctgcgcc
    33001 ggtgcccccc gtccgcgccg tgcttctggc ggcgatgctc gccctggcgc tcgccgcgcc
    33061 ggccgccgcc gctccgttcc agctcacgcc caccggcggc gaccccgacg tggccgtcga
    33121 ctcgagcgga accgctcacg tcgtctggaa ccagccgagc tccgagggct ccgacgtgac
    33181 ggtctactgc cagctcccgc gcggcgccac cacgtgctcg cggttccaga acctgatccc
    33241 cgcgggggag aagctgttcg acgcggcggg gccgcaggtg gtgctcgcgc cagacggccg
    33301 gatcgcggtg gcgaccgggc gctgctgcag ccaggtgtgg cggttcgtct cggccgacgg
    33361 cgggcagacg ttcgactcgc ggctggtcgg cgacctgacg ggcgtctccg actggcgggc
    33421 cgccttcggc cccggggagc ggatctcgct cgtcaacgcc ggtacctacc aggcgccgcc
    33481 gctcgacggc tcgacgaacg cgaaggcgga gctactgccg ggctcgatct acggcacggg
    33541 cctcacgatg ctcgactcga acacgccgct cgtggtggcg tcgatgaccg gcgaacgcga
    33601 ccagctcggc tggcgccgct acgacgggtc gggcgacccc aacgacgcag gcaactggac
    33661 ccccgcgcag ccgctcggcc ccggcacgga cccgcacctg gtgaccggcc cgcgcggcac
    33721 ctacctgatc tacgaggccg gcgtcgagag caacgccgcg tacgtgatcc gccgctggaa
    33781 cggcgcgggt ttcgacccgg tcgcggcgac ggtgatttcg gccggcggcc agcagcgctc
    33841 gcccgccttc caccaagacc cgaccggcac cttccacgcg gtgttcggga gcaacgacac
    33901 gatgccgcag cgcctcctct actcgagctc gcccgacggg acgacctggt ccgcaccgcg
    33961 cgagatcgcg ccctaccgcg agtccggcta cttccacaac gagatagccg ccgcggggcc
    34021 cgacgacggc ctggccgtga acgacgtgaa cgagagcgcc ggcaacgtcg aggcagcccc
    34081 gctgtcggcg caggccgccg agcccgagcg ccccgacgat ccctcctgcc gcacgcgcgc
    34141 ggagttccgg ccggtgaagg tcgtgctccc cgagggctgc ttcaagcagg tgagcggacg
    34201 gctcgagacg agcgacaagg tgaaggtgaa cggcctcctg atcgagccgc gcgccggcgc
    34261 gaccgtcgtg atcgaccagg ccaagcggcg gatccactcg cgcggcgtca cgacggtgcg
    34321 cgccggcctg attccggtgc aggtcggggt gctcgactgg tcgatcaagg acgccgcgaa
    34381 gtcgagcgtc gacacgttcg acctcggcga cgccgggggc aaggtgttcg gcttcccgct
    34441 gaccgccacc ggcgaggtcg acttcgactc cggcacgacc acgatcgcgg cccacgtcgg
    34501 cctgccgaag ttcctcggcg gggtgaccgg tgacatcacg ctgcgcaccg acaacgacaa
    34561 cggcctgcgg ctcgacgggc tcgtcatcaa ggtgccgagc gcgctgatcg gcccggtcca
    34621 ggtcaagggc ctcgtgctca gctacatggc ggccggcagc gtctgggagg gctcggccac
    34681 gctgctcctg ccgccgagcc cgcccggccc gaagctcgac gccgccatcg gcttccgcga
    34741 gggcggcttc gactacggcc gcggcgagta caccttcacg agtcccggcc tcgggctggg
    34801 cgccggcgtg ttcctcaaca agatccgctt cgcggtgaac ctccagccgg cgccgccgcg
    34861 aatcgcgggc ggagtcacgc tcagcgccgg gccgaccgtg gccggccaga aggcgctcgg
    34921 gatcgagggc gacatctcgt acacgttccc gaacccgccc gcgcccgcgg tcctgcgcgc
    34981 tgacggcacc gccacgctca gctcgatccc gttcctgaag gcgtacgcgg agtaccggtc
    35041 gagcggcttc ttcgcgttcg gcgccgagct caactacttc ttcgtgaagg acctcgtggg
    35101 gatcaacgcc gccgtggacg gttggttcac gagcagcgcg ttcaacatcg agggcaaggg
    35161 aaccgtgtgc gtcggctact gcttcggggc ggaggcggtg ctctcgagcg aggggatcgc
    35221 cgcgtgcggg cgggtgccgg tctgggggac ggaggtcgcg ctcggcttcg gctacaagtg
    35281 gagcggcggg ctcagcatca tgctgctgtc gtgcgacgtc gggccgttcc gcgccgtcca
    35341 gtcggcgcag gcgggcggcg agtcggagtt cacgctcggg tcggggctgc cggtggcgac
    35401 cgtcgcgttc cgcggcgcga ccgcgccgcc gaaggtcgtg ctcgagggcc ccggcggcga
    35461 gcgcgtcgag accccggccg cggccgacgg caaggtgctc gacgagcgct tcctgctctt
    35521 ccagaatccc gacgacaaga cgacctacgt cgcgatcaac cgcccgtcgg cggggcgctg
    35581 gaaggccagc gtcgccggcg ccgcggtgtc ccctcggctc gacgtgcggg ccgcgcagtc
    35641 gtcgccgatc gccgacatcc aggtggcgac cggcctgccg aagccgtcgg tcaaggcgcg
    35701 cgtgagcggc agcggccact cgcgcaggct cacctacagc gtgaagtcga tccccggcca
    35761 gaaggtgcac ttcgaggagg tggccgcgac cgggggtggg cgccggatcg gcaccgcgaa
    35821 gggcaagacc gggacgctcc gcttcagccc cgccgacggg ccgaagggca agcgcaggat
    35881 cgtcgcgctc atcgagtcca acggcattcc gcgcgagaac atcgacgtgg ccacctacac
    35941 cgcccccgca ccggcgcgcc cggcgcggcc cgccggcgtg aagctgcaac gcaagggctc
    36001 gcgcgtgacc gtcacgtggc ggccggccgc ccgctcggcg cgctacctcg tgcgcgcgaa
    36061 cctctccgac gggcgcaggc tgctcctgtt cgccaccgcg aagaagcgca gcgtgcggtt
    36121 caccgggctg ccgaccagcg ctcgcgggac gatcacggtc gtgggcgtga agcccgacgg
    36181 cacgcccggc tcggccgcga gggcgaagct cgccccgcgc aagcgccgct gatcgcggct
    36241 cgctgccgcg cgcgcaaggg ggctcgctgc cgcgcgcgcg ggccatgtag tctcgcgccg
    36301 tcttcggatc gggagggtgc gaatggcggg actcagggga gtggcgatcg cgagcggcgc
    36361 agccgcgctg gtgctggcgg ccggctgcgg aggcggcggc gcgaagaagc aggagaagcc
    36421 ggcgacccca gccgagaccc ccgtgaacga cccgccggtg caggccaagg agtcgggcgc
    36481 gcccagcgac ctgctcggcc agtacacggt gaagttgccc aaggccgacc tcccgaagga
    36541 cgcgcccgag gagctcaccg agggcagcga gacctggacc gtcacgctcg ccgagaccgg
    36601 cggccccggc gaggggcccg ccttcaccat cgcgaacgac cagctgggcg cgctcgagag
    36661 ctcgaacttc gaggtcaagg gcgatcggat cctcctccac gaggaggaat gcgcccagac
    36721 gggcgagccg gtcgagagcg agtggaccta cgagctgaag ggcgagcggc tgacgctgaa
    36781 ggaggcgaag ggcgcgtgcc cggacgaggt ggcgtccacg atcctcacga cgagcccgct
    36841 ccagcgaaag aagtaacgcg gcgcccgcgt gcgcatcccc tggaagctga tcgggcttgc
    36901 cgggctggcg ggcgtggccg cgaccggtgt cgtcgttgtg cgcaggcgcc gccgcgccca
    36961 gcgcgactac tcgcccgacg agctgcgcgc ccggctgcac cagcggctcg ccgaggtgcc
    37021 gcactgaacc tcgacgaggc cgtggccgag gcggccaccg gcgacatctg gctcttccgc
    37081 gggcgctcgc ttgccgaccg cgcgatccgt acggtcacga acagcccggt gaaccacgtc
    37141 gggatggtgg tggcgatcga cgacctgccg ccgctcctgt ggcacgccga gctcggccgc
    37201 tcgctgcccg acgtgtggac cggcgagcac cagcgcgggg tgcagctcca ccgcctcggc
    37261 gaggcggtcc gcacctggaa cgagcgctac agccagcggg cgtggttccg gccgctcgag
    37321 gggacggtcg agcgcagcca cgaggaccgg ctcatggagg tgatcgagcg cttcgacggg
    37381 cgcccgttcc cgaccacgct cggcctggcc cggcagtggg tcaacggccg cttccgccgc
    37441 tcgtcctcgc gcgaggcgat ctactgcgcc gagctggtgg cgaccaccta ccagcacatg
    37501 gggctgctgc cgagccgccg gccggcgagc tggtacgacc cgggtcgctt ctggagcggc
    37561 gaccggatcg agctggtcgc gccgttcgcg ctggcgggcg aggtggcggt ggactgaggg
    37621 gcggcgccca caacgacgag ggggagcccg atcggctccc cctcgaagcg ttgcgttctg
    37681 accgcggccg gtctagccgc ccgagcccgt cggcacgacg tagttgccgc gcacgatgtt
    37741 ggcccgcggc gggttgtcgc cgaagtccgc gcagtctggg tctgcgacct cgtccgggaa
    37801 cgcgaagcgc cagcggtgct ggtcggcgcc gtggccgggc tgaccgttgt cgacgacctc
    37861 gatcaggatc ggcagcagca cacccggtgc cacctcgttg cggccgagcc cgccgacgag
    37921 cgcggtgttg ccctcctgcc tcaggcaggt gacgtcaccc tggaacggcg tgcccacggc
    37981 gtcggacacg aaccagtgac cgcgcggccc gtcgccctgg tccttcgcgt tcacgtgcac
    38041 cgcgccgtac tccgggctct cgcccgtgcc gtcggccttc tcgaacgtcg gcgccgcgcc
    38101 cgcgacgacc ggtgtcgcag cgatcgccat gacggcgagt gcgacgaaca gaagcattcg
    38161 cttcatgctc ttgccccctc ttgagttagg aactacatac ccctgcggga ggcacatccc
    38221 caccgggtgc gggaggcaaa ccccgcgcgg gcgtcaggcg gcttcgccga tgcccgcgcc
    38281 aaagctctcc tcggctgcca gggcgacgat ccgcacatgc gagcgggcga ccatcaggaa
    38341 cgacgcggtg cgggtctgct ccggggcgtc cagcgcggtg accgtcgcgt cgttcagcgc
    38401 gaagaactcg cgatcgcgct ggttgacgta gtccgagagg cggctgcggt agccctcgcg
    38461 cggaagcgtg agcttgccct cgatccggta acggtcggtc tcgaggatga tgtcctcgtg
    38521 gcgcatgtcc atggcgcgga ctctatcggc gcccggagcg gacgggcgtg cagaccgacc
    38581 tgccgagccg cggcagcagc tcgcgcgcga ctcgctcgcc cgacctgacg gcgccgtcca
    38641 tgtagccgtt ccagtacgtc gaggtctcgg tgccggccca gtgaatccgg ccgaccggtg
    38701 cgcggatcgc ctcgccgtag tcgagcagca cgccgggcgg cgcggcgcag accgggcagc
    38761 cgcgcgtcca gagctcgctg tcccagagcg cctccacgta gcccgtcgcg ttcagcgcct
    38821 cttcgccgaa gtagctcgcg tagttctcga gcacgagccg gcggcgctcc tcggggccgg
    38881 tcctgtcgag ccgccgcgcg gtggtcgcct cgacgaagcc catgatcacg ccgcgcgagc
    38941 cgtccggggg cgagttgtcg aaggtgacct gcaccgggcc ggtgtcgctg accacctgcc
    39001 cgttcaggcc gcggtcgcgc cagaagggcc gctcgtagac cgcctggacc ttgatgaccg
    39061 atcccatcgg catccgctgg gtgagctgct cgcgcatccc cggcagcttc gggctgtagt
    39121 cgatccggcc ggcgaggtgc ggcgggatcg cgacgaccac gcgctgaccg gtggcggtga
    39181 cgccgttcgc gtggacctcg acggacccct tgcgctgcac gatgcgcctg accggcgcat
    39241 tcaggcggat ccgcttgccg agccggcggg ccagctcgag cggcacgcgc tgcgagccgc
    39301 cgtggaagcg cagctcctgc gcgccgccgg cggtgttcac aagccggtcg agggtgccgg
    39361 gcgtcccctc ggtgcccgcc gccgcgatgt agaagaggac gaacagcatc gacatgtcgc
    39421 gcggctcgaa cgagaagatc gcggtggtgg ccacgtcgat caggaagcgc ccgccctcgg
    39481 tg
//
LOCUS       DASVPL010000303         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_389660, whole genome shotgun sequence.
ACCESSION   DASVPL010000303 DASVPL010000000
VERSION     DASVPL010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389660"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VF517_17430"
     CDS             complement(<1..502)
                     /locus_tag="VF517_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054773.1"
                     /translation="MTKYLIAALAAGLLVVGTAVAALAPGGSESPATTQEDVTSTTEQ
                     PGEVSGPCDEAEHANDPRCAGDGTARREDDDARREDRVDDDDDAGEVRGPCDEAEHAN
                     DPRCTGAAGEQRGNDGSRRSNSGPGSAHRRGRGRGRGRGGDDAPQAPAPSGDDSASRS
                     GSGSGSG"
     gene            complement(634..1551)
                     /locus_tag="VF517_17435"
     CDS             complement(634..1551)
                     /locus_tag="VF517_17435"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8054774.1"
                     /translation="MAPNPLRALARATPAPFDGIVELGAAGIDDLVAAARVIDRGLPG
                     NSLDEWDPEHIRRTLPLNKALVRTYFRSEVTGMENIPDGPSLLVGNHSGGTLIADTFA
                     FAYEFYDHFGPDRRFHQLAHDVAARMPGFGMLRRYGTLAASHENAKKAFAAGAPVLVY
                     PGGDWETFRPSWHSDRIEFGGRKGFIKLALDEGVPIVPVVAIGGQETALFITRGRTLA
                     KITGLDRFARIKVLPIAIAPPFGISVLDLPTRLPLPAKIKVEVLPPIDLRERFGPNPD
                     PDDVYIEITDEMQDSLSSLSEERTAPVLG"
     gene            complement(1612..2016)
                     /locus_tag="VF517_17440"
     CDS             complement(1612..2016)
                     /locus_tag="VF517_17440"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEX8054775.1"
                     /translation="MPTGPIVPDTSAWIEFIRGTGSVANARLRAFQQFGADLVVTGPV
                     VMEVLAGAQREAQRSRARDLLGRCRYIAVEDPSDYEHAAALYRACRVAGQTLRGQLDC
                     LIAAVAIRIGAAVLHADSDFDVIARHSPLRIV"
     gene            complement(1991..2227)
                     /locus_tag="VF517_17445"
     CDS             complement(1991..2227)
                     /locus_tag="VF517_17445"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX8054776.1"
                     /translation="MARITVEVDDHVLRNAMAMLRCKTEQEAVDQALRRVAYNPLRGN
                     PMSLEEALAMEGTGWEGDLDEMRFNEHADWTDRP"
     gene            complement(2266..>2959)
                     /locus_tag="VF517_17450"
     CDS             complement(2266..>2959)
                     /locus_tag="VF517_17450"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX8054777.1"
                     /translation="AEQARLFAQRPDPRWVAPPGRSLHRLGTELDLGPAGAYGWLARN
                     APRFGFIKRYSWEPWHFGFTRSPGTASVGFGVRGGDGRATAALQSFVPARYAPMIARA
                     AQRWSVSAQLLAAQIYVESNFNPFARSPAGAQGIAQFMPGTAHGMGLRNPFNPAQAID
                     AQAHLMRDLLRRFGSVPLALAAYNAGPGAVARCGCIPPYPETRGYVSKILGLLGGAGE
                     VLAGGFTVRLVK"
BASE COUNT          404 a         1000 c         1137 g          418 t
ORIGIN      
        1 agccgctgcc gctgcctgag ccggagcgcg aggcggagtc gtcaccgctg ggcgcggggg
       61 cctgcggggc gtcgtcgcca ccgcggccac ggccacggcc cctgccgcga cggtgggcgc
      121 taccggggcc ggagttcgag cgacgcgagc cgtcgttacc gcgctgctcg ccggcggcgc
      181 cggtgcagcg ggggtcgttg gcgtgctcgg cctcgtcgca cgggccgcgc acctcacccg
      241 cgtcgtcgtc gtcgtcgacg cgatcctcgc ggcgagcgtc gtcgtcctcg cggcgcgcgg
      301 tgccgtcgcc cgcgcagcgc gggtcgttgg cgtgctcggc ctcgtcgcac gggccgctca
      361 cctcgccggg ctgctcggtc gtgctggtca cgtcctcctg cgtggtcgcc gggctctcgc
      421 tgccgcccgg ggcgagcgcc gcgacggcgg tccccaccac gaggagcccg gcggcgagtg
      481 ctgcgatcag gtacttggtc atcgggtcca acctctcttc cggagtgccg tgccgtgact
      541 gtgcccggcc gagaccagcg cgcgggcagg cgtgtccgag gggacgggaa ggcttcggtt
      601 aggagttggt aaagccgccg tgtggcggcg cgatcagccc aggacggggg cggtgcgctc
      661 ctcggagagc gagctcagcg agtcctgcat ctcgtcggtg atctcgatgt agacgtcgtc
      721 cggatccggg ttcgggccga agcgctcgcg gaggtcgatc ggcggcagga cctcgacctt
      781 gatcttcgcc gggagcggga gccgggtcgg cagatcgagg acactgatcc cgaacggcgg
      841 ggcgatggcg atcggcagga ccttgatgcg ggcgaagcgg tcgaggccgg tgatcttcgc
      901 gagggtgcgc ccgcgcgtga tgaagagcgc ggtctcctgg ccgccgatgg cgacgaccgg
      961 cacgatcggc acgccctcgt cgagcgcgag cttgatgaag cccttgcggc cgccgaactc
     1021 gatccggtcg gagtgccagc tggggcggaa ggtctcccag tcgccgccgg ggtacacgag
     1081 cacgggcgcg cccgcggcga acgccttctt cgcgttctcg tgggaggccg cgagggtgcc
     1141 gtagcggcgc agcatgccga agccgggcat ccgcgccgcg acgtcgtgcg ctagctggtg
     1201 gaagcggcgg tcgggcccga agtggtcgta gaactcgtag gcgaaggcga atgtgtccgc
     1261 gatcagcgtg ccgccggagt ggttgccgac cagcagcgac ggcccgtccg ggatgttctc
     1321 catgcccgtg acctcggagc ggaagtacgt gcgcacgagc gccttgttga gcggcagcgt
     1381 gcggcggatg tgctcgggat cccactcgtc gagcgagttg ccgggcaggc cgcggtcgat
     1441 gacgcgtgcg gcggcgacga ggtcgtcgat gccggcggcg ccgagctcga cgatgccgtc
     1501 gaacggcgcg ggggtcgcgc gcgcgagcgc gcggagtggg ttcggagcca tgtcaggttc
     1561 gtccccggag gggcggtcgg gcaaacggcg cgcgggcttc cccgcccacc gctagacgat
     1621 ccggagaggc gagtgccggg cgatgacgtc gaagtcgctg tcggcgtgga ggacggcggc
     1681 gccgatgcgg atggcaacgg cggcgatcag gcagtccagc tggccgcgca gggtctggcc
     1741 cgccacgcgg catgcgcggt agagcgcagc cgcgtgctcg tagtcgctcg gatcctcgac
     1801 cgcgatgtaa cggcagcggc cgagaaggtc gcgggcgcgg gaacgctgcg cctcccgctg
     1861 ggcccccgcc agcacctcca tcactacagg cccggtgacg accagatccg cgccgaactg
     1921 ttggaacgcg cgtagacgcg cattcgcgac gctacccgtc ccccggatga actcgatcca
     1981 ggccgacgtg tcagggacga tcggtccagt cggcatgctc gttgaaacgc atctcgtcca
     2041 ggtcgccctc ccacccggtg ccctccatcg cgagagcctc ctccagagac atagggttgc
     2101 cgcgcaaggg gttgtaggcg actcgtcgca gcgcctggtc cacggcctcc tgctcggtct
     2161 tgcagcggag catcgccatg gcgttgcgca ggacgtggtc gtcgacctcg accgtgatgc
     2221 gtgccatcga gtccatcgta cgcctcgatt cgcgccgcaa ccccatcact tcaccagccg
     2281 cacggtgaag ccaccggcga gcacctcacc ggctccgccc agcaagccga ggatcttcga
     2341 gacgtagccg cgcgtctccg gatagggcgg gatgcagccg cagcgcgcga ccgcgcccgg
     2401 cccggcgttg tatgcggcca gcgcgagcgg tacggagccg aagcggcgta gtaggtcgcg
     2461 catcagatgc gcctgggcgt cgatcgcctg cgccgggttg aatggattgc gcagacccat
     2521 gccgtgggct gtgccgggca tgaactgggc gatgccctgc gcgcctgcgg gcgagcgcgc
     2581 gaacgggttg aagttcgact cgacatagat ctgcgccgcg agcagctggg ccgacacgct
     2641 ccagcgctgc gcggcgcggg cgatcatcgg cgcgtagcga gcggggacga aggactggag
     2701 cgcggccgtg gcacggccgt cgccgcccct gacgccgaag ccgaccgagg cggtgccggg
     2761 gctgcgcgtg aagccgaagt gccagggctc ccacgagtag cgcttgatga acccgaaccg
     2821 cggagcgttg cgcgcgagcc agccgtacgc gccggccggg ccgaggtcga gctcggtgcc
     2881 gagccggtgc aacgagcgcc cgggcggcgc cacccagcgc ggatcggggc gttgtgcgaa
     2941 cagccgggcc tgctcggcg
//
LOCUS       DASVPL010000304         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_389699, whole genome shotgun sequence.
ACCESSION   DASVPL010000304 DASVPL010000000
VERSION     DASVPL010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389699"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..271)
                     /locus_tag="VF517_17455"
     CDS             complement(47..271)
                     /locus_tag="VF517_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054778.1"
                     /translation="MISDISPLEWSLGIAGTITLICYVVFIVAPAWASYGRVWEKFAA
                     SVLTLFILASLLMVGVLLGAAVVWTYDTWA"
     gene            complement(304..1131)
                     /gene="ispE"
                     /locus_tag="VF517_17460"
     CDS             complement(304..1131)
                     /gene="ispE"
                     /locus_tag="VF517_17460"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX8054779.1"
                     /translation="MIEERAPAKLNLVLHVGARRADGLHELASLFASLDLADVVALEE
                     APTAPDEVICPGVEEPNLAGAAIDALRAQLGGDLPPLRVAIEKRIPVAAGLGGGSADA
                     AAVLRAANHLAGDPLDASALRRLGATIGADVPSQIEPRHALVTGAGEHVEPVELAPMA
                     VVLVPAPVGLSTAAVYAEADRIGSTRASLDPAALRALADLPPAELAPRLENDLEPAAC
                     SLRPELEDTLTAVERTGALAARVTGSGPTVFGVYRDAVTAAEAAAALPGAIRTTLQP"
     gene            complement(1128..2147)
                     /gene="rsmA"
                     /locus_tag="VF517_17465"
     CDS             complement(1128..2147)
                     /gene="rsmA"
                     /locus_tag="VF517_17465"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX8054780.1"
                     /translation="MTATPDRDAAAPRQASLRRLREFGVRPNRELGQNFLIDDNVLGV
                     IGRAAELDTSDVVLEVGGGLGVLSEYLAPRVGHLHVVEIDAALEPALRDALGPHANVT
                     LHLADAVRLDLTALAPEPTKVVANLPYGVAATVILRVLAELPAVERVVAMVQREVAER
                     FAAGPGSKTYGGTSVLAQLSAEVRVERRVPASVFHPAPRVESAIVVMRRTAPPPPRAL
                     VDLVHAAFAHRRKALSGSLALAADAEPAIRDRARAALEELGHPADARAERLAPADFAA
                     LAARLAPGAADPRGSAAADPPGSAAADPQGSAADPPGFPADLPGSPAGSAADIPRSRS
                     PEAPS"
     gene            complement(2144..>2759)
                     /locus_tag="VF517_17470"
     CDS             complement(2144..>2759)
                     /locus_tag="VF517_17470"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX8054781.1"
                     /translation="AAREHEEVFAIVGHHPNDASSFDDASLAAIDAAGRTDHAVAIGE
                     TGLDYYRDYSPRDVQMRAFEAQIDVARELALPLVIHTRAAEDDTFAVLRDRADGVTVV
                     MHCFSAPARVDECVERGYVCSFAGNVTYPKAQDLQAAARDVPAELLMVETDAPYLAPQ
                     PVRGKPNEPANVVRTAEYVAELRGVSYAELEASVESTAARVFGW"
BASE COUNT          372 a          964 c         1104 g          319 t
ORIGIN      
        1 ggccgctcgc gccggggaca gctcctttga ccccggcgcg ccggggtcag gcccaggtgt
       61 cgtacgtcca gacgaccgcc gcgccgagca gcactccgac catcagcaac gaggcgagga
      121 tgaacagcgt gaggacgctg gcggcgaact tctcccagac ccggccgtag gaggcccagg
      181 ccggagcgac gatgaacacg acgtaacaga tcagcgtgat cgtgcccgcg attccgagcg
      241 accactcgag cggcgagatg tccgagatca cggttggcga ttctgacgca tgcgggcgac
      301 gagtcagggc tggagggtgg tccggatcgc gcccggcagg gcggcggcgg cctccgccgc
      361 ggtgacggcg tcacggtaga caccgaagac cgtggggccc gagccggtca cgcgcgccgc
      421 gagcgcgccg gtgcgctcga cggcggtgag agtgtcctcc agctccggcc gcagcgagca
      481 cgccgcgggc tcgaggtcgt tctcgagcct cggggcgagc tcggcgggag ggaggtccgc
      541 aagggcgcgt agggcggcgg ggtcgaggct cgcgcgcgtg gagccgatcc ggtccgcctc
      601 ggcgtagacg gcggcggtcg agaggcccac gggcgcgggc acaaggacca cggccatcgg
      661 cgcgagctcg accggctcga cgtgctcgcc ggccccggtg acgagggcgt ggcgcggctc
      721 gatctggctc ggcacgtcgg cgccgatggt ggcgccgaga cggcgcagcg cggacgcgtc
      781 gagcgggtcg cccgcgaggt ggttggcggc gcgcaggacc gcggcggcgt cggcgctccc
      841 gccaccgaga ccggcggcga ccgggatgcg cttctcgatg gccacgcgca gtggcggcag
      901 gtcgccgccg agctgggccc ggagcgcgtc gatcgccgcg ccggcgaggt tcggctcctc
      961 caccccggga cagatgacct cgtcgggagc ggtcggcgcc tcctcgaggg cgaccacgtc
     1021 ggcgaggtcg agcgacgcaa acagcgatgc gagctcgtgg agcccgtcgg cgcggcgggc
     1081 gccgacgtgg aggacgaggt tgagcttcgc gggcgcgcgc tcctcgatca cgagggggcc
     1141 tcgggggagc gggagcgcgg gatgtccgcg gcggagccgg cgggggaccc gggcaggtcg
     1201 gcggggaacc cgggcgggtc ggcggcggag ccttgcgggt cggcggcggc ggagccgggc
     1261 gggtcggcgg cggcggagcc gcgcgggtcg gcggcgcctg gcgcgaggcg cgccgcgagg
     1321 gcggcgaagt cggccggggc gagtcgctcc gcgcgtgcgt ccgccggatg gcccagctcc
     1381 tcgagtgcgg cgcgcgcacg gtcgcggatc gccggctccg catccgccgc gagagcgagc
     1441 gagcccgaca gcgccttgcg cctgtgcgcg aacgcggcgt ggaccaggtc gaccagcgcg
     1501 cgcgggggcg gcggggcggt ccggcgcatc acgacgatcg cggactcgac gcgcggcgcc
     1561 ggatgaaaga cggatgccgg gacgcggcgc tccacccgca cctcggccga gagctgcgcg
     1621 agcacggacg tcccaccgta ggtcttcgag cccggccccg cggcgaaccg ctcggccacc
     1681 tcgcgctgga ccatcgccac gacgcgctcc acggccggca gctccgccag cacgcgaagg
     1741 atcacggtgg ccgcgacgcc gtacggcagg ttggccacga ccttggtcgg ctccggggcg
     1801 agggcggtga ggtccagccg cacggcgtcg gcgaggtgga gcgtgacgtt cgcgtgcggt
     1861 ccgagcgcgt cgcgcagcgc cggctcgagc gccgcgtcga tctccaccac gtgcagatgc
     1921 ccgacgcgcg gcgcgaggta ctccgacagc accccgaggc cgccacccac ctcgagcacc
     1981 acgtccgacg tgtcgagctc ggccgcccgg ccgatcacgc cgaggacgtt gtcgtcgata
     2041 aggaagttct ggccgagctc gcgattcggt cgcaccccga actcgcgcag ccggcggagg
     2101 ctggcctggc gaggcgcggc ggcgtcgcga tccggcgtcg cggtcaccac ccgaacacgc
     2161 gggcggcggt cgactcgacc gacgcctcga gctccgcgta cgacacgccg cgcagctccg
     2221 cgacgtactc cgccgtgcgc accacgttcg ccggctcgtt cggctttccg cgaacgggct
     2281 ggggagccag gtatggcgcg tcggtctcga ccatcagcag ctcggcgggg acgtcgcggg
     2341 ccgcggcctg gaggtcctgt gccttcggat aggtgacgtt gccggcgaag gagcagacgt
     2401 agccgcgctc gacgcactca tccacgcgcg cgggagcgga gaagcagtgc atcacgacgg
     2461 tgacgccgtc ggcgcggtcg cggagaacag cgaaggtgtc gtcctccgcc gcgcgcgtgt
     2521 gtatgacgag cggcagggcc agctcgcgcg ccacgtcgat ctgcgcctcg aacgcgcgca
     2581 tctgtacgtc gcgcggcgag tagtcgcggt agtagtcgag tcccgtctcg ccgatcgcaa
     2641 ccgcgtgatc ggttcggccg gcggcgtcga tcgccgccag cgaggcgtcg tcgaagctcg
     2701 acgcgtcgtt cgggtggtgg ccgacgatcg cgaacacctc ctcgtgctca cgcgcggcg
//
LOCUS       DASVPL010000305         4712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_75965, whole genome shotgun sequence.
ACCESSION   DASVPL010000305 DASVPL010000000
VERSION     DASVPL010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75965"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..2424
                     /locus_tag="VF517_17475"
     CDS             127..2424
                     /locus_tag="VF517_17475"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX8054782.1"
                     /translation="MRAHLALDRARRGYGAEGSLALRLAAFWALLAFAAGHWVGFVAD
                     PPVGRAVLAVAIATAAGGALAASARVSAPRPLRVALRVLIAVVGLNAGLLAIGLEPRF
                     LLPGHWDELAVGVDGGLAGTASAGWPYDGDDAWLRLTVLLGLPTAGTLAAVLAFWPAR
                     RGTGVLRAAALILIVALYALAVTERGLGEPVGRGLVLLGLAAAWLWLPRLRGRDAAAA
                     AVALLVAAAVAIPVSTGLHEREGWVAYENWALFGNDGRGDGSTFDWSHNYGPIDWPRD
                     GKTLLRVRSEHSHYWKAETLDHFDGLRWLHSTATTDIDAQSDVPEDHRRSWDERIGFE
                     VEALRSGFLVTAGTVWRVGGDLLTAESGDGTIELVDEELGSGDNYGVYAYVPDPSARQ
                     MRRAPRVFPSQFLSYTTLELPEPGETALGEGAPKGPYTPDPAQVVRSFLPGTPLGADP
                     ETRARIEASPYARTYALARRLAAGQPTSYDVVKRIERYFEHGFVYNERPPVRAIPLDG
                     FLFRDRNGYCQQFSGAMALLLRMNGIPARVAAGFSPGIRDQETGEYRVRDYDAHSWVE
                     VFFTGIGWVPFDPTPSLSPAAAQASDARQASASQGGSDRGDTAGISRPVHDVDLSGGG
                     AEEGGKSSLWIALALVLALPLAVLGGLWVVALVRARRVRRSGGDPDVRELVWALDRLG
                     HPVAPGTTLLGLERRLAGIGGPAAARHARALRQRRFAPPGPVRDAAGLDRRALRRALT
                     RGRGPLTRVRALMALPPRRPPFTQG"
     gene            2451..3701
                     /locus_tag="VF517_17480"
     CDS             2451..3701
                     /locus_tag="VF517_17480"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8054783.1"
                     /translation="MDRRASSASHLVSGALGGLVAVVLGAILIATGVIDTGDETVVRQ
                     GDTARPVAGGNGRDAATVNEIYRRTDAGVAFIQASGGSTGASPIPGLPQPPEGQGRST
                     GSGFALDDEGFILTNAHVVEGADEVRVRFGDQDSVKVQVRGADPSTDIALLKVDPGKV
                     KLRPIPLGDSSKVRVGDQAIAIGNPFGLEHTVTTGIVSALQRSIDAPNGFSIENAIQT
                     DASINPGNSGGPLLDGDGRVIGINAQIETGGGSRGSVGIGFAIPINLAKRVIPQLKEK
                     GKVEHAYIGVTTAPVTEGTEGIDLPADKGALVQDVAPESPAAKAGLRAGKTQTDEGLV
                     VGGDLIVKVDGIAIDEPPDVARAIADNKPGDRIAIEYYRGKKLRTVVLTLGERPKRVP
                     QQGGRGQEPDQPDEPKPDKPFQLP"
     gene            3721..4365
                     /locus_tag="VF517_17485"
     CDS             3721..4365
                     /locus_tag="VF517_17485"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX8054784.1"
                     /translation="MTRVKVCGVAELEDARLAVELGAWAVGMVFHPESPRACDPGTAE
                     LIGGELRRRAELVGVFVNWPLEELEHMADRASLTMLQLHGDEGPAYCREAARRTGAKV
                     MKAMQAKDAAAVRKLASYREVDLHMLDAHTDDLRGGTGRTFEWQLAANRRSHVPLVLS
                     GGLNPDNVGAAIEAVRPYAVDSASGTEAEPGRKDPAKLRALFGAVAQADAEQAA"
     gene            4362..>4712
                     /locus_tag="VF517_17490"
     CDS             4362..>4712
                     /locus_tag="VF517_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054785.1"
                     /translation="MSTHAVEHRFGPYGGRYVPETLVPALDELERAWVSAREDAASGE
                     ELGAIAGVLAHADPGRLELGERRHQRLGDVLAAVGPVAVLDARLDAAHDATSSSTAGA
                     GSARASGAGSGPAAG"
BASE COUNT          589 a         1828 c         1714 g          581 t
ORIGIN      
        1 gatcgtcacg ccgtcggacg gacctccgcc gccgggcggc ttcagcgtcg ccgggtgcac
       61 cgggcgcctg ctcggggccg ccgcgtcgag ggcggcatga gcgcggcggc acacggcctc
      121 ggccgcgtgc gtgcccatct cgcactcgac cgcgcgcgcc gtggctacgg ggccgagggc
      181 tcgctcgccc tccggctggc cgcgttctgg gcgctgctcg cgttcgcggc cgggcactgg
      241 gtcggcttcg tggccgaccc gcccgtgggg cgcgcggtcc tggccgttgc catcgccacc
      301 gcggctggtg gcgccctcgc tgcctccgcg cgggtgagcg ccccgcggcc gctgcgggtc
      361 gcgctccgcg tgctgatcgc cgtcgtcggg ctgaacgccg ggctcctcgc catcggcctc
      421 gagccccgct tcctcctgcc gggccactgg gacgagctcg ccgtcggtgt cgacggcggc
      481 ctcgccggca ccgccagcgc cggctggccc tacgacggcg atgacgcgtg gctgcggctg
      541 accgtgctcc tcggcctgcc cacggccggc acgctcgccg cggtcctcgc cttctggcct
      601 gcacgccgcg gcaccggtgt cctgcgcgcg gccgcgctga tcctgatcgt ggccctctac
      661 gcgctcgcgg tgaccgagcg cggcctgggt gagcccgtcg gccgcggcct ggtgctgctc
      721 gggctcgccg ccgcctggct gtggctcccg cgcctgcgcg ggcgcgacgc cgccgcggcc
      781 gccgtcgcgc tcctcgtggc ggccgccgtc gcgatccccg tctccaccgg attgcacgag
      841 cgcgagggct gggtcgccta cgagaactgg gccctgttcg ggaacgacgg ccgcggcgac
      901 ggctcgacgt tcgactggtc gcacaactac ggcccgatcg actggccgcg cgacggcaag
      961 acgctgctgc gggtgcgttc ggagcactcg cactactgga aggccgagac cctcgaccac
     1021 ttcgacggcc tgcgctggct gcactcgacc gcgaccaccg acatcgacgc ccagtccgac
     1081 gttcccgagg accaccggcg gtcctgggac gagcgcatcg gcttcgaggt cgaggcgctg
     1141 cgcagcggct tcctcgtgac cgccgggacc gtctggcgcg tgggcggcga cctgctcacc
     1201 gccgagagcg gcgacgggac gatcgagctg gtcgacgagg agctcggcag cggcgacaac
     1261 tacggcgtgt acgcctacgt ccccgatccc agcgcgcgcc agatgcgccg cgcgccgcgc
     1321 gtcttcccgt cgcagttcct ctcgtacacg accctcgagc tgcccgaacc gggggagacg
     1381 gcgctcggcg agggcgcccc gaagggcccg tacacgccgg accccgcgca ggtcgtgcgc
     1441 tcgttcctcc cgggcacgcc cctcggggcc gaccccgaga cgcgcgcccg gatcgaggcc
     1501 tccccgtacg cgcgcaccta cgcgctcgcc cggcgcctcg ccgccggtca gccgacgagc
     1561 tacgacgtgg tgaagcggat cgagcgctac ttcgagcacg gcttcgtcta caacgagcgc
     1621 ccaccggtgc gcgcgatccc gctcgacggc ttcctgttcc gcgacaggaa cggctactgc
     1681 cagcagttct ccggcgcgat ggcgctgctc ctgcggatga acggcatccc cgcgcgggtg
     1741 gcggcgggct tctcgccggg gatccgcgac caggagaccg gcgagtaccg cgtgcgcgac
     1801 tacgacgcgc actcgtgggt cgaggtcttc ttcacgggca tcggctgggt cccgttcgac
     1861 ccgacgccgt cgctctcgcc ggccgcagct caggcctccg acgcacggca ggcgagcgcg
     1921 tcgcagggcg ggagcgacag gggcgacacc gccgggatct cccggcccgt ccacgacgtc
     1981 gacctcagcg gcggcggcgc cgaggagggc gggaagtcgt cactgtggat cgccctggcc
     2041 ctggtgctcg cgctgccgct ggcggtcctc ggcgggctct gggtcgtcgc gctcgtgcgc
     2101 gcgcgccgcg tgcggcgctc gggcggcgac ccggacgtgc gcgagctcgt gtgggcgctc
     2161 gaccggctcg gccatccggt cgcgcccggc accacgctgc tcggcctcga gcgccggctc
     2221 gcgggcatcg gcgggcccgc cgccgcccgg cacgcgcgcg ccctgcgcca gcgccgcttc
     2281 gccccgccgg gaccggtgcg cgacgccgcc ggcctcgacc gccgagcact gcgccgcgcg
     2341 ctcacgcgcg gccgcggccc gctcacgcgc gtgcgcgcgc tgatggcgct gccgccgcgc
     2401 cggcccccgt ttacacaggg ctaacctcgc ccccgtagaa ccccggcccc atggaccgtc
     2461 gcgcctcctc cgcatcccac ctcgtctccg gcgcgctcgg cgggctcgtc gcggtcgtgc
     2521 tcggcgccat cctcatcgcc accggagtga tcgacaccgg cgacgagacg gtggtccgcc
     2581 agggcgatac ggcgcgcccc gtggccggcg gcaacggccg cgacgccgcc acggtcaacg
     2641 agatctaccg ccgcaccgac gccggcgtgg cgttcatcca ggcgagcggc ggcagcaccg
     2701 gcgcgtctcc gatccccggg ctgccccagc ccccggaggg ccagggccgc tcgaccggct
     2761 cgggcttcgc gctcgacgac gaggggttca tcctcacgaa cgcgcacgtg gtcgagggcg
     2821 ccgacgaggt gcgcgtgcgc ttcggcgacc aggactccgt gaaggtccag gtcaggggcg
     2881 ccgacccctc gaccgacatc gcgctgctga aggtcgatcc cggcaaggtg aagctgaggc
     2941 cgatcccgct cggcgactcg agcaaggtcc gcgtgggcga ccaggcgatc gcgatcggca
     3001 acccgttcgg gctcgagcac acggtcacga cgggcatcgt ctccgcgctc cagcgctcga
     3061 tcgacgcacc caacggcttc tcgatcgaga acgcgatcca gaccgacgcc tcgatcaacc
     3121 cgggcaactc gggcggcccg ctgctcgacg gagacggccg cgtgatcggg atcaacgccc
     3181 agatcgagac tggcggcggc agccgcggct cggtcggcat cggcttcgcg atcccgatca
     3241 acctcgcgaa gcgggtcatc ccgcagctca aggagaaggg caaggtcgag cacgcctaca
     3301 tcggcgtgac caccgcgccg gtcaccgagg gcacggaggg aatcgacctg ccggccgaca
     3361 agggcgcgct cgtccaggac gtggcgccgg agagcccggc ggccaaggcg ggcctgcggg
     3421 ccggtaagac ccagaccgac gaggggctcg tcgtcggcgg cgacctgatc gtgaaggtcg
     3481 acggcatcgc catcgacgag ccgccggacg tcgcccgcgc gatcgccgac aacaagccgg
     3541 gtgacaggat cgcgatcgag tactaccggg gcaagaagct gcgcactgtc gtgctgacgc
     3601 tcggcgagcg ccccaagcgc gtcccgcagc agggcggccg cggccaggag cccgaccagc
     3661 cggacgaacc caagccggac aagcccttcc agctcccctg acgcacctac tattgccccg
     3721 gtgacacggg tcaaggtatg cggcgtcgcc gagctcgagg acgcgcggct ggcggtcgag
     3781 ctcggcgcct gggcggtggg gatggtcttc caccccgaga gtccgcgcgc gtgcgacccg
     3841 ggcaccgcgg agctgatcgg cggcgagctc aggcgcaggg ccgagctcgt gggggtgttc
     3901 gtcaactggc cgctcgagga gctcgagcac atggccgacc gcgcctcgct cacgatgctc
     3961 cagctccacg gcgacgaggg ccccgcgtac tgccgcgagg ccgcgcggcg cacgggcgcg
     4021 aaggtgatga aggcgatgca ggccaaggac gccgcggccg tgcgcaagct cgcctcctac
     4081 cgcgaggtcg acctccacat gctcgacgcg cacaccgacg acctccgcgg cggcaccggc
     4141 cgcacgttcg agtggcagct cgccgcgaac cgccgctcgc acgtgccgct cgtgctgagc
     4201 gggggactga acccggacaa cgtcggcgcg gcgatcgagg cggtgcgccc gtacgccgtc
     4261 gacagcgcca gcggcaccga ggccgagccc ggccgcaagg acccggcgaa gctgcgcgcc
     4321 ctgttcgggg ccgtggcgca ggccgacgcc gagcaggccg cgtgagcacc cacgcggtcg
     4381 agcaccgctt cggcccgtac ggcggccgct acgtgcccga gaccctcgtg cccgcgctcg
     4441 acgagctcga gcgggcctgg gtctcggccc gtgaggacgc cgcgtccggg gaggagctcg
     4501 gcgcgatagc cggggtcctc gcgcacgccg acccaggtcg cctcgagctc ggcgagcgcc
     4561 ggcatcagcg tctcggggac gtactggccg ccgtagggcc cgtagcggtg ctcgacgccc
     4621 gcctcgacgc ggctcacgac gccacctcct cctcgacggc cggcgcgggc tcggcgaggg
     4681 cctccggcgc gggctcgggg cccgccgccg gc
//
LOCUS       DASVPL010000306        13872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_192140, whole genome shotgun sequence.
ACCESSION   DASVPL010000306 DASVPL010000000
VERSION     DASVPL010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13872
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192140"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VF517_17495"
     CDS             <1..464
                     /locus_tag="VF517_17495"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX8054786.1"
                     /translation="LTALVLGAGGAGRAAAWALSDAGAAEVAVWNRTEDRAAALAGEL
                     GVAHAARPRQADLLVNATSVGLDPATSEADALAQLDLTGVDPPPLVVDLVYRAGSATP
                     VQAWAERAGATVVDGLEVLVRQGARSFELWTGQPAPVEAMRAAARKTSGNT"
     gene            498..1052
                     /locus_tag="VF517_17500"
     CDS             498..1052
                     /locus_tag="VF517_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054787.1"
                     /translation="MALPRPVLIALVGLAALMALFLATRHGAQNESVTPPSTAAKPAT
                     TKAPAADKPARPESRQAAAPKAAEPVTPRKPKLDDGVPPQVRKAAEALGRDQVVVFFF
                     TKPGAADDTGARIAVRSIEGMKGVALFKAGIEEVGAYRPMLSQLEISQVPATAIVRPG
                     KKAVLLQGFVDAGTLRQNVADARR"
     gene            1049..2905
                     /locus_tag="VF517_17505"
     CDS             1049..2905
                     /locus_tag="VF517_17505"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX8054788.1"
                     /translation="MNEPQAPAELRSLGLDELGEELPAVDLAGTAASAGDSDLPAVDA
                     SEDGAADAFTGITPPRRRGGAARFITDVIVELGFLPQAKVDAAVEEGKATGRSPEDVL
                     LQAGVLSADELARATAERFGLDHVDLTVYKPDIAAVNLLSPQAARRYNAVPVGFHTNG
                     HLLVAMADPSNVLAIDDLKLMTGYEIRATVSSTDDIAGLIVKMNRLDEAVAEAVETDE
                     DEDDQALVAEIRESATDAPTIKLVNSIIAQAVEDGASDIHFEPHNKDMRVRFRVDGVL
                     HETTTVPRRMVPGVVSRVKIMADLDIAEKRLPQDGRVTLQVEGHHVDVRIVTMPQAGG
                     EAIVMRLLDKESVMLTLDALGIRDGARGRFEDGVRKSHGAVLVTGPTGSGKSTTLYAA
                     LNTVNSIEKNIITIEDPVEYQIPGINQMQVNLKHGLTFAAGLRSMLRADPDIIMVGEI
                     RDAETARIAIEAALTGHLVLSTLHTNDAPSAITRLSEMGIAPFLTASALDVVVAQRLA
                     RRLCNYCKRRTVLTAEVLKAANFHEAAFDIEAYEPVGCARCSHTGYKGRVGIYEVMKV
                     TDEIREMTIERRDASHIGTKAVEQGMRLLRDDGLEKVRLGVTSIAEVARVAS"
     gene            2938..4104
                     /locus_tag="VF517_17510"
     CDS             2938..4104
                     /locus_tag="VF517_17510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX8054789.1"
                     /translation="MNFDFADILIEVIESEASDLHLTAGSPPMVRKKGKLSALDYPRL
                     SSQDTREIVYSILNNEQRKRLEVDWQIDLSYSVPNRARFRVNAFFQRASIGAVMRTIP
                     TVIPALADLGLPPVLEDLTKKPRGFVLVTGPTGSGKSTTLAAMIDLINKQRHEHILTI
                     EDPIEFLHPHQNCIVNQRELGTDASSFAAGLKAALRQDPDVILVGEMRDLETMATALT
                     AAETGHLVFATLHTQDTAQTVDRIVDAFPPEQQHQVRVQLSIALQGIVTQQLLPTRDG
                     KGRCCATEVLVPTAGVRNLIREGKTHQIYSALQTGAAHGMQTMDASLVDLVRQGKIDQ
                     AMAERRSSTPAELKRLIGSNGGGGGMIGGMGGNGNGGMGSMQPTMGAPAQGAMR"
     gene            4108..5316
                     /locus_tag="VF517_17515"
     CDS             4108..5316
                     /locus_tag="VF517_17515"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8054790.1"
                     /translation="MGTFTFKAVDLQGVPSRGELDAESKQSVSQQLRSKGLIVLDIEE
                     LKNSDISFGRFKKVKAQELTIATRQLATMIQSGMSLLRALYVIEEQARSDKFREAITS
                     VRQDVEAGISLSDALGRFPDIFNGLYIAMVSAGEVGGILEQTLDRIADQLEKDDSLRR
                     QVKAAMMYPCLIGGFAMVVLIALVVFLVPVFEKVFADFGGELPPITKFTVGMSNLITG
                     RWYLCIAVVFGVVYGFKKWKRTDKGLAQWDRFKLRIPWKIGDLVQKVCLARFSRTYSA
                     LIGAGVPMLQAIEITGKTSGNKVIENAMRQVGESVRAGGSIATPMRAEPNAFPIMVTQ
                     MIGVGEETGALEQMLSKIADFYEDEVAAGLKALTSILEPLMIIVVGGIVGFIVVAMYM
                     PMFKVYDSIK"
     gene            5357..5707
                     /locus_tag="VF517_17520"
     CDS             5357..5707
                     /locus_tag="VF517_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054791.1"
                     /translation="MDGRTLEWRDEQGRLHRTDGPARVFASGREEWYRHGRLHRVGGP
                     AVLHANGSEKWYEDGVRHRDGAPACVYVNGTEKWYRRGLRHRDDGPAASYPDGRRIWF
                     VDGVKVREERVAPG"
     gene            5721..7106
                     /locus_tag="VF517_17525"
     CDS             5721..7106
                     /locus_tag="VF517_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054792.1"
                     /translation="MRVCVAIAALTLLIGSVPTYDPWAWMVWGRELAALDLDTSVGPA
                     FKPLPVLVGVFGAPWLWLVIARAGALVAVAMAWRLGHRLGGGSLLAAAVAGAGVLLCA
                     GWVWNGMLGNAEGLMLALVLVAFARALDGRHRQAFALGFLAALLRTEVVPFLALYAAW
                     LWRREPGSQMRPLIVAAFAALPVLWLGPDLIGSGDLLRSSERARIPNPGAPALADRPA
                     LASLGRAIALAPTMVWGGAALAVLGAARGELARAAALPAAAGVAWMALVAAMSELGYS
                     GEERYALPGVALVAVSAGAGAAWAVGRVAARAAREVTSVPNRDAFDFGGGSGRPFAVA
                     RWLPVAVGLVLLGAAAGESAGALRGHVRDLRYESRLYGTLDGAVAAAGGREAVLRCGP
                     VHTAPYSRPALAWRLRVPMSALSTEPASRGIAFRARPHRSAAIGPRLEGQGWSTRGRP
                     GAWTVVERCRP"
     gene            7103..8632
                     /locus_tag="VF517_17530"
     CDS             7103..8632
                     /locus_tag="VF517_17530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8054793.1"
                     /translation="MRPRLDTRTVSAAALVLLLGASALLRTRAIGAGYWIDEGISVGI
                     AGHAFADIPGLLAQDGSPPLYYLLLSLWTSVAGSGETATHALSLIAALATVPAALWAG
                     RALFGTRAGWAAAAIAATLPLLTVHAQETRMYALIALLSLLLSATFVLAFMRGSGRAL
                     IAFGVLAVLTAYTHNWGLYLLAGLAAALPLAVRERGARPVLRDAALAGGATALAYAPW
                     IPTLIDQARHTGAPWSTTPDLGGLVNPVSVPLGEGVAAVLVVAVAVAALVRARQSAAA
                     ALALALVVAGALAWGLAQVEPGWADRYMTVFAGPVILLAGAGLAAAGRAGMAVLAVAV
                     ILWALDPAPESKSNAREVARVLAPGDLVVVTHPEQVPVLRHYAPSAAIRWASALGPVA
                     DPRVFDWRDAPARLNEAEPRAVLGRLLDGTGPAGAEAAPRRRAGEGKAGAAPRRLVLI
                     APARPRTKGPEWIDLVARTAAEWSALVAADPRLARVASAEPEPIRGTSLKLDAYRLRA
                     P"
     gene            8657..10099
                     /locus_tag="VF517_17535"
     CDS             8657..10099
                     /locus_tag="VF517_17535"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8054794.1"
                     /translation="MPPSTPPVAVLGGGPAGLTAGLMLARRGIPVVVLEAERQVGGLA
                     KTVERDGYRFDLGGHRFFTKSTEVSALWHELLGDELLLRPRLSRIRWRGRFLDYPLRA
                     GDVVRKLGPVELSRALGSYARAAAVPNAREESFEEWVVNRFGRRLFELFFRSYTEKVW
                     GVPTSDLRAEWAAQRIRNLSFATAARAAVLGNGGGIKSLIEEFHYPRFGPGQMWETMA
                     DEIVLAGGELRLGTPVTGIELDPDGGAVAAVHAGGERIEARAAISSLPLSALPRLAGA
                     PERVTRAAGGLRHRDFLTVALVLDGPDPFPDTWVYVHDPSVHVARIQNFRAWSPWMVP
                     EPDRSCVGLEYFCFEDDELWRRPDGELVELAAAELERTGLASAARVVRGHVVRVPRAY
                     PVYAGDYAERVATIRAWLDGLGNLQQVGRGGLHRYNNSDHSMLTAMRAVENLLDGAEH
                     DVWAVNADGAYHEEYGPDEQPYREPVAAAP"
     gene            10096..11184
                     /locus_tag="VF517_17540"
     CDS             10096..11184
                     /locus_tag="VF517_17540"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8054795.1"
                     /translation="MKRALALAAALAIAGCGGGGDGGGGPKAPNGGAPAGGPVKPGDL
                     FELKEVAAGFDQPLGLVQAPGEDTLYVVEQPGRVVSLDRRKTLIDIADRVQSGGEQGL
                     LGLAFDPGYARNGRFYLHYTNKDGDTRVDEYTGTARRELLAVDQPFPNHNGGQLSFGP
                     DRRLYLGLGDGGSANDPHDNGQDLGARLGKILRFDGTRWEVAAYGLRNPWRFSWDRST
                     SSLWIADVGQDQREEIDVVPGLGGPPPNFGWPAFEGGNDPDDRKPSGPGRLVFPVAEY
                     DHDEGCSVTGGYVYRGRAVPGLRGRYVYGDVCSGRLWSLRAGRDSATDVRREDDELEQ
                     LSSFGEDAAGELYAVSLDGRVFRLGASD"
     gene            11234..12226
                     /locus_tag="VF517_17545"
     CDS             11234..12226
                     /locus_tag="VF517_17545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8054796.1"
                     /translation="MTATAPMIELDRVTKRFGAVDAVSATRLTVERGELLAVLGPSGC
                     GKTTMLRLIAGFEFPDGGTVTIDGRIVSGRDWVPPERRQVGMVFQDYALFPHLSVAEN
                     VGYGLPRKRRAPRVAEVLALVGLSGLEERRPHELSGGQQQRVALARALAPEPSIVLLD
                     EPWSNIDPLLRGAMRDDIARILRTAGVTVVLVTHDQEEAFSLADRIALMDAGSIVQVG
                     TPEEMYYQPVSRRVAEFVGATNFVAAESPLGRDLVQGEAGEVLVRPELVGLTIDEAGG
                     GTVVGREFRGHDVFYRVELGDGGSICSQRPSNEVVPLGARVRVELHHGPVKVFS"
     gene            12226..13248
                     /locus_tag="VF517_17550"
     CDS             12226..13248
                     /locus_tag="VF517_17550"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEX8054797.1"
                     /translation="MKRLLALALALTALALSACGGDDGGGDGVSGGPDKLTIYSGRNE
                     ALIGGLFDQFEKDAKIDVEVRYGDSAELAATLSEEGGNSPADLFFSQDAGALGAVEKQ
                     LRTLPAALVNRVDPRFRDDQRRWVGVSGRARVLAYSTERLKPADLPKSVFDMTDPRWK
                     GKLGIAPPNASFQAFVSAMRIDVGDDRARKFLEGIKANDPHLYENNLQAVEAIADGEI
                     DVALVNHYYLYEIRRERGDVPVDNHFFQTGDPGSLVNAAGVGVLKSSDSASAAQRLVD
                     YVLSKKGQTYFAEKTAEYPLAAGVKPGPELRPLNSIHGPFIDLGMLGEKLPSTLEMIE
                     EAGLTN"
     gene            13248..>13872
                     /locus_tag="VF517_17555"
     CDS             13248..>13872
                     /locus_tag="VF517_17555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX8054798.1"
                     /translation="MPAAAAPAAPARRRPPVVLGLVAAAVAAMVCLPLVYLAIRAGGG
                     GADAWDVLWRERTGLLLWRTVVLVGAVTAAAVAIGVPLAWLVTRTDLPGRRIWAVAAA
                     LPLVIPSYVTALALLSAFGAGGVIESVPDITGFTGSFAALTLATYPYVFLLSAGALRQ
                     LDGSLEEASRALGRTRTYTFFRVTLPVIRPSVAAGALLVALYAISDFG"
BASE COUNT         1877 a         5180 c         4892 g         1923 t
ORIGIN      
        1 ggctgaccgc gctggtgctc ggcgcgggcg gcgcggggcg cgccgcggcc tgggccctga
       61 gcgacgcggg cgccgccgag gtggccgtct ggaaccgcac ggaggaccgc gccgcggccc
      121 tcgcgggcga gctcggcgtc gcgcacgccg cgcgcccgcg ccaggccgac ctgctcgtga
      181 acgcgacgtc ggtgggtctc gacccggcca cgagcgaggc cgacgcgctc gcgcagctcg
      241 acctcaccgg ggtcgatccg cctccgctgg tggtcgacct ggtctaccgc gccgggtccg
      301 cgaccccggt ccaggcctgg gccgagcgcg ccggcgcgac cgtggtggac ggcctcgagg
      361 tgctcgtccg ccagggcgcc cgcagcttcg agctgtggac cgggcagccc gctccggtgg
      421 aagccatgcg ggccgccgcg agaaaaacct cgggcaacac ctaaacaacc gtcgtagccg
      481 tgccgataca gcgggcgatg gctcttcctc gccccgtcct gatcgcccta gtcggcctcg
      541 cagcactgat ggcgctcttc ctcgcgaccc gccacggcgc gcagaacgag tcggtcaccc
      601 cgccgtccac cgccgccaag cccgccacca ccaaggcccc ggctgccgac aagcccgcgc
      661 ggcccgagtc gcgccaggcc gcggccccga aggccgccga gcccgtcacc ccgcgcaagc
      721 cgaagctcga cgacggcgtg ccgccgcagg tgcgcaaggc cgccgaggcg ctcggtcgcg
      781 accaggtcgt cgtcttcttc ttcaccaagc ccggtgccgc agatgacacc ggtgctcgca
      841 tcgccgttcg ctcgatcgag gggatgaagg gcgtcgcgct gttcaaggcg ggcatcgaag
      901 aggtcggcgc ataccggccc atgctctccc agctcgagat ctcgcaggtg cccgccacgg
      961 cgatcgtccg ccccggcaag aaggccgtcc tgctccaggg cttcgtcgac gccggcaccc
     1021 tccgccagaa cgtcgcggac gctcggagat gaacgagcca caggccccgg ccgagctccg
     1081 gtccctaggg ctcgacgagc tcggtgagga gctgcccgcg gtcgacctcg ccggcaccgc
     1141 cgcgtccgcc ggcgactccg atctcccggc cgtggacgcc tccgaggacg gcgccgctga
     1201 cgccttcacc ggcatcaccc cgccgcgccg ccgcggcggt gccgcccgct tcatcaccga
     1261 cgtcatcgtc gagctcggct tcctgccgca ggcgaaggtc gacgcagccg tcgaggaggg
     1321 caaggccacc ggccgctcgc ccgaggacgt gctgctccag gccggtgtcc tgagcgccga
     1381 cgagctcgcc cgcgcgaccg ccgagcgctt cggcctcgac cacgtcgacc tcaccgtcta
     1441 caagcccgac atcgccgcgg taaacctgct cagcccgcag gcggcccgcc gctacaacgc
     1501 ggttccggtc ggcttccaca cgaacggtca cctgctggtg gcgatggccg atccgtcgaa
     1561 cgtcctcgcg atcgacgacc tcaagctgat gacgggctac gagatccgcg cgacggtctc
     1621 ctcgaccgac gacatcgccg gcctgatcgt gaagatgaac cgcctcgacg aggcggtcgc
     1681 cgaggccgtg gagaccgacg aggacgagga cgaccaggcg ctcgtcgcgg agatccgcga
     1741 gtcggccacc gacgcgccga cgatcaagct cgtcaactcg atcatcgccc aggcggtcga
     1801 ggacggcgcc tcggacatcc acttcgagcc ccacaacaag gacatgcgcg tccgcttccg
     1861 cgtcgacggc gtgctccacg agaccacgac cgtgccgcgc cggatggtgc cgggcgtcgt
     1921 ctcccgcgtg aagatcatgg ccgacctcga catcgccgag aagcgcctgc cgcaggacgg
     1981 ccgcgtgacg ctccaggtag agggccacca cgtcgacgtc cgcatcgtca cgatgccgca
     2041 ggccggcggc gaggcgatcg tcatgcgtct gctcgacaag gagtcggtga tgctcacgct
     2101 cgacgcgctg ggcatccgcg acggcgcccg cgggcgcttc gaggacggcg tccgcaagtc
     2161 gcacggcgcg gtgctcgtca ccggcccgac cggctccggc aagtcgacca cgctctacgc
     2221 cgcgctgaac acggtcaaca gcatcgagaa gaacatcatc acgatcgagg acccggtcga
     2281 gtaccagatc cccggcatca accagatgca ggtgaacctc aagcacggcc tgacgttcgc
     2341 ggccggcctg cgctcgatgc tgcgcgccga ccccgacatc atcatggtcg gcgagatccg
     2401 ggacgccgag accgcgcgca tcgcgatcga ggccgccctc accggccacc tcgtcctctc
     2461 caccctccac acgaacgacg cgccgtccgc gatcacgcgg ctcagcgaga tgggcatcgc
     2521 gccgttcctc acggcctcgg ccctcgacgt cgtcgtcgcc cagcgcctcg cgcgccggct
     2581 ctgcaactac tgcaagcgcc gcaccgtcct caccgcagag gtgctgaagg ccgccaactt
     2641 ccacgaggcc gcgttcgaca tcgaggccta cgagccggtc ggttgcgcgc gctgctcgca
     2701 caccggctac aagggccggg tcggcatcta cgaggtcatg aaggtcacgg acgagatccg
     2761 cgagatgacc atcgagcgca gggatgcctc gcacatcggc accaaggccg tcgagcaggg
     2821 aatgcgcctt ctgcgcgacg acggcctcga gaaggtgcgc ctcggcgtca cctcgattgc
     2881 ggaggtcgct cgcgtcgcgt cctaaaggga cgcccgcgaa cgccgaaaga acccctgatg
     2941 aacttcgatt tcgccgacat cctcattgag gtcatcgagt ccgaggcatc ggacctgcac
     3001 ctgaccgcgg gctcgccgcc gatggtgcgc aagaagggca agctcagcgc gctcgactat
     3061 ccgcggctgt cctcgcagga cacgcgcgag atcgtctact cgatcctgaa caacgagcag
     3121 cgcaagcgcc tcgaggtcga ctggcagatc gacctctcct actcagtccc gaaccgcgcc
     3181 cgcttccgcg tcaacgcctt cttccagcgc gcctcgatcg gcgccgtgat gcggacgatc
     3241 ccgaccgtga tccccgcgct cgcggacctc ggcctgccgc cggtgctcga ggatctcacc
     3301 aagaagccgc gcggcttcgt gctcgtcacg ggcccgacag gctccggcaa gtccacgacc
     3361 ctcgccgcga tgatcgacct gatcaacaag cagcggcacg agcacatcct cacgatcgag
     3421 gatccgatcg agttcctgca tccgcaccag aactgcatcg tgaaccagcg cgagctgggg
     3481 accgacgcgt cgtcgttcgc cgcgggcctc aaggccgcgc tgcgccagga ccccgacgtg
     3541 atcctcgtcg gcgagatgcg cgacctcgag acgatggcga ccgcgctgac ggccgccgag
     3601 accgggcacc tcgtcttcgc cacgctccac acccaggaca ccgctcagac ggtggaccgg
     3661 atcgtcgacg ccttcccgcc cgagcagcag caccaggtgc gcgtgcagct ctcgatcgcc
     3721 ctccagggaa tcgtcaccca gcagcttctc ccgacgcgcg acggcaaggg ccgctgctgc
     3781 gccaccgagg tgctcgtccc gaccgcgggc gtgcgcaacc tgatccgcga gggcaagacc
     3841 caccagatct actcggcgct ccagaccggc gccgcgcacg gcatgcagac gatggacgcg
     3901 tcgctcgtgg acctcgtccg ccagggcaag atcgaccagg cgatggccga gcgccgctcg
     3961 tccaccccgg ccgagctcaa gcggctcatc ggcagcaacg gcggcggcgg cggaatgatc
     4021 ggcgggatgg gcggcaacgg caacggcggc atgggctcca tgcagccgac catgggcgcg
     4081 cccgcgcagg gagcgatgag gtaggtcatg ggcacgttca cgttcaaggc ggtggacctc
     4141 cagggcgtgc cgtcgcgcgg tgagctcgac gccgagagca agcagtcggt cagccagcag
     4201 cttcgctcga aggggctgat cgtcctcgac atcgaggagc tgaagaacag cgacatcagc
     4261 ttcggccgct tcaagaaggt caaggcccag gagctgacga tcgcgacgcg ccagctcgcg
     4321 acgatgatcc agtcgggcat gtcgctcctc cgcgcgctgt acgtgatcga ggagcaggcc
     4381 cgcagcgaca agttccgcga ggcgatcacc agcgtgcgcc aggacgtcga ggccggcatc
     4441 tccctctccg acgcgctcgg ccgcttcccc gacatcttca acggcctcta catcgccatg
     4501 gtctcggccg gcgaggtggg cggcatcctc gagcagacgc tcgaccggat cgccgaccag
     4561 ctcgagaagg acgactcgct gcgccgccag gtgaaggccg cgatgatgta tccgtgcctc
     4621 atcggcggct tcgccatggt ggtgctgatc gcgctcgtgg tcttcctggt gccggtgttc
     4681 gagaaggtct tcgcggactt cggcggcgag ctgcccccga tcacgaagtt cacggtcggg
     4741 atgtcgaacc tcatcacggg caggtggtac ctctgcatcg ccgtggtctt cggcgtggtc
     4801 tacggcttca agaagtggaa gcggaccgac aagggcctcg cgcagtggga ccgcttcaag
     4861 cttcgcatcc cgtggaagat cggcgacctg gtccagaagg tctgcctcgc gcgcttctcc
     4921 cgcacctact cggcgctgat cggagccggc gtgccgatgc tccaggccat cgagatcacc
     4981 ggcaagacct cgggcaacaa ggtcatcgag aacgcgatgc gccaggtggg cgagtcggtc
     5041 cgcgccggtg ggtcgatcgc gaccccgatg cgcgccgagc cgaacgcctt cccgatcatg
     5101 gtcacgcaga tgatcggcgt cggtgaggag accggcgcgc tcgagcagat gctctcgaag
     5161 atcgcggact tctacgagga cgaggtggcc gccggactga aggccctgac ctcgatcctc
     5221 gagcccctca tgatcatcgt cgtgggcggc atcgtcggct tcatcgtcgt ggcgatgtac
     5281 atgccgatgt tcaaggtcta cgactcgatc aagtagcgcc cgcggggctt gatcgcccgc
     5341 cggtaccgtc ctggagatgg acggccgcac cctcgaatgg cgtgacgagc agggccgcct
     5401 gcatcgcacg gacggcccgg cgcgggtgtt cgcgtccggg cgcgaggagt ggtaccggca
     5461 cgggcggctt caccgcgtcg gcgggcccgc cgtattgcac gcgaatggct cggagaagtg
     5521 gtacgaggac ggcgtgcgcc accgcgacgg cgcgcccgcc tgcgtgtacg tgaacgggac
     5581 cgagaagtgg taccggcgcg ggcttcgcca tcgcgacgac gggccggccg cctcgtaccc
     5641 cgacgggcgg cgcatctggt tcgtggacgg ggtgaaggtg cgcgaggagc gcgtcgcgcc
     5701 gggctagcct gcgcgccgtg ttgcgcgtgt gcgtagcgat cgctgccctg acgctgctga
     5761 tcggctcggt ccccacctac gacccgtggg cgtggatggt gtgggggagg gagctcgccg
     5821 cgctcgacct cgacacgtcg gtgggcccgg cgttcaagcc gctgccggtg ctcgtcggcg
     5881 tcttcggcgc cccgtggctg tggctcgtca tcgcccgggc gggcgccctg gtggccgtgg
     5941 cgatggcgtg gcggctgggg caccggctcg gcggcggatc gctgctcgcg gccgcggtgg
     6001 ccggcgcggg cgtcctgctg tgcgcggggt gggtgtggaa cggcatgctg gggaacgcgg
     6061 aggggctgat gctcgcgctc gtgctggtcg cgttcgcgcg cgcgctcgac gggcgccacc
     6121 ggcaggcgtt cgcgctgggg ttcctcgccg cgctgctgcg gaccgaggtg gtcccgttcc
     6181 tcgcgctcta cgcggcgtgg ctgtggcggc gggagcccgg gtcgcagatg cgaccgctca
     6241 tcgtcgccgc cttcgcggcg ctccccgtcc tgtggctcgg gcccgacctg atcggctcgg
     6301 gcgacctgct ccgctcgtcc gagcgcgcac ggatccccaa ccccggggcc ccggccctcg
     6361 cggaccgccc cgcgctcgcg tcgctcggcc gcgcgatcgc gctggcgccc accatggtct
     6421 ggggcggcgc ggcgctggcg gtcctcggcg ccgcgcgcgg cgagctcgcc cgcgcggccg
     6481 cgctaccggc ggcggccggg gtcgcctgga tggcgctggt cgcggcgatg agcgagctcg
     6541 gctactcggg cgaggagcgc tacgcgctgc ccggcgtggc gctggtcgcg gtgtcggccg
     6601 gagccggcgc tgcgtgggcg gtggggaggg tggccgcgcg ggcggcacgc gaagtcactt
     6661 ccgtccctaa tagggacgct tttgacttcg ggggcggatc gggccgcccg ttcgcggtcg
     6721 cgcgctggct ccccgtcgcc gtcgggctcg tgctcctggg tgctgcggcc ggggagagcg
     6781 cgggcgctct gcgcggccac gtgcgcgacc tgcgctacga gtcgcgcctg tacggcacgc
     6841 tcgacggcgc ggtcgccgcc gcgggcggcc gcgaggccgt gctgcgctgc gggccggtgc
     6901 acaccgcgcc ctactcgcgc ccggcgctcg cctggagact gcgcgttccg atgagcgcgc
     6961 tgtcgaccga acccgcgagc cgcggcatcg cgtttcgcgc gcggccccac cgcagcgcgg
     7021 cgatcgggcc gcggctcgag ggccaggggt ggagcacgcg cggtcgtccg ggcgcgtgga
     7081 ccgtcgtgga gcgctgccgc ccgtgaggcc gcggctcgac acgcgcacgg tctcggccgc
     7141 cgcgctcgtc ctgctgctcg gagcgtccgc gctgcttcgc acccgcgcga tcggcgcggg
     7201 ctactggatc gacgagggga tctcggtcgg catcgccggg cacgcgttcg cggacattcc
     7261 cggcctgctg gcccaggacg gctcgccgcc gctctactac ctgctgctct cgctgtggac
     7321 gagcgtcgcc ggcagcggcg agacggccac ccacgccctc tcgctcatcg ccgcgctcgc
     7381 gaccgtcccc gccgccctct gggccgggcg cgcgctgttc ggcacccggg ccgggtgggc
     7441 cgccgcggcc atcgccgcca cgctgcctct gctcacggtc cacgcgcagg agacgcgcat
     7501 gtacgcactt atcgcactcc tgtccctcct cctgagcgcg acgttcgtgc tcgccttcat
     7561 gcgcgggagc ggccgcgcgc tgatcgcatt cggcgtgctg gccgtcctca ccgcctacac
     7621 ccacaactgg ggcctctacc tgcttgccgg gctggccgcg gcgcttcccc tggccgtgcg
     7681 cgagcgcggc gcgcggccgg tgctgcgcga cgcggcgctc gccggcggcg cgaccgccct
     7741 cgcgtacgcg ccgtggatcc ccaccctgat cgaccaggcg cgccacaccg gcgcgccgtg
     7801 gtcgaccacg cccgacctcg gcggcctcgt gaacccggtg tcggtgccgc tgggcgaggg
     7861 cgtcgcggcc gtgctggtgg tcgccgtcgc cgtcgccgcg ctcgtgcgcg cgcggcagtc
     7921 cgcggcggcg gcgctcgccc tggccctggt cgtggcgggc gcgctcgcgt ggggcctcgc
     7981 ccaggtcgag cccggctggg ccgaccgcta catgacggtc ttcgccggac cggtgatcct
     8041 gctcgccggc gccggcctgg ccgcggcggg ccgcgccggc atggcggtgc tcgcggtggc
     8101 cgtgatcctc tgggcgctcg accccgcgcc cgagtcgaag agcaacgcgc gcgaggtggc
     8161 tcgcgtgctc gcgcccggtg acctcgtggt ggtcacgcac cccgagcagg tgccggtcct
     8221 gcgccactac gcgccgagcg cggcgatccg ctgggcgagc gcgctcggcc cggtcgcgga
     8281 cccgcgcgtg ttcgactggc gcgacgcgcc cgcgcgcctg aacgaggcgg agccgcgcgc
     8341 ggtgctcggg cggttgctgg acgggacggg accggcgggt gcagaagccg ctccgcgtcg
     8401 gagagctggg gaggggaaag ccggcgccgc gccgcgccgt cttgtcctaa ttgctccggc
     8461 gcggccccgc accaaaggcc ccgaatggat cgacctcgtg gccagaaccg ccgccgagtg
     8521 gagcgcactg gtcgccgcgg acccgcgact cgcgcgcgtg gcgagcgccg agccggagcc
     8581 gatccgcggg acctcgctga agctcgacgc ctatcgactt agggcaccct aagtaccccg
     8641 tatactgccg cggccgatgc ctcccagcac gccacccgtc gccgtactcg gcggcggccc
     8701 ggccggactg accgcggggc tgatgctcgc ccgccgtggc atcccggtcg tggtgctgga
     8761 ggccgagcgc caggtcggcg gcctcgccaa gaccgtcgag cgcgacggct accgcttcga
     8821 cctgggcggc caccgcttct tcaccaagag caccgaggtg agcgcgctct ggcacgagct
     8881 gctcggcgac gagctgctcc tgcgcccgcg cctctcgcgc atccgctggc gcggccgctt
     8941 cctcgactac ccgctccgcg cgggcgatgt cgttcgcaag ctcggacctg tcgagctgag
     9001 ccgcgcgctc ggctcctatg cccgcgccgc cgccgtcccg aacgcgcgcg aggagtcctt
     9061 cgaggagtgg gtcgtgaacc gcttcggccg ccgcctgttc gagctcttct tccgctccta
     9121 caccgagaag gtctgggggg tcccgacctc cgacctgcgt gccgagtggg ccgcgcagcg
     9181 gattcgcaac ctctcgttcg cgaccgccgc gcgggcggcc gtgctcggca acggcggcgg
     9241 gatcaagagc ctgatcgagg agttccacta cccccgcttc ggcccgggcc agatgtggga
     9301 gacgatggcg gacgagatcg tgctggcggg cggcgagctg cggctcggca cgccggtcac
     9361 ggggatcgag ctcgacccgg acggcggcgc cgtggccgcg gtccacgcgg gcggcgaacg
     9421 catcgaggcc cgcgccgcga tctcctcgct gccgctgagc gcgctgccac gcctggccgg
     9481 cgcgccggag cgcgtgacgc gcgcagccgg cggcctgcgc caccgcgact tcctgaccgt
     9541 cgcgctcgtg ctcgacggac cggacccgtt ccccgacacc tgggtctacg tgcacgaccc
     9601 gtccgtgcac gtcgcccgca tccagaactt ccgcgcctgg agcccgtgga tggtccccga
     9661 gccggaccgc tcctgcgtcg ggctcgagta cttctgcttc gaggacgacg agctgtggcg
     9721 ccggccggac ggcgagctcg tggagctggc cgccgccgag ctcgagcgga ccggtctcgc
     9781 gagcgctgcc cgcgtggtcc gcgggcacgt ggtgcgcgtg ccgagggcgt acccggtcta
     9841 cgcgggcgac tacgccgagc gggtcgcaac gatccgcgcc tggctcgacg gcctcggcaa
     9901 cctccagcag gtcggccgcg gcggcctcca caggtacaac aactccgacc actcgatgct
     9961 gaccgcgatg cgcgcggtcg agaacctgct cgacggcgcc gagcacgacg tctgggcggt
    10021 caacgccgac ggcgcctacc acgaggagta cgggccggac gagcagccct accgcgagcc
    10081 cgtcgcggcc gctccgtgaa gcgggcccta gcgctcgccg ccgccctggc gatcgccggg
    10141 tgcggcgggg gcggcgacgg cggcggggga ccgaaggccc cgaacggcgg cgcgcccgcg
    10201 ggcggcccgg tcaagcccgg cgacctcttc gagctgaagg aggtcgccgc ggggttcgac
    10261 cagccgctcg gcctcgtcca ggcgcccggc gaggacaccc tctacgtggt cgagcagccc
    10321 ggccgggtcg tctcgctcga ccgccgcaag accctgatcg acatcgccga ccgcgtgcag
    10381 agcggcggcg agcagggcct gctcggcctc gccttcgatc ccggctacgc acgaaacggc
    10441 cgcttctacc tccactacac caacaaggac ggcgacaccc gcgtcgacga gtacaccggc
    10501 accgcccgcc gcgagctgct cgccgtcgac cagccgttcc ccaaccacaa cggcggccag
    10561 ctcagcttcg ggcccgaccg gcggctctac ctcggcctcg gcgacggggg ctcggccaac
    10621 gacccgcacg acaacggcca ggacctggga gcgcgcctgg gcaagatcct gaggttcgac
    10681 ggcacgcgct gggaggtggc cgcctacggc ctgcgcaacc cctggcgctt ctcgtgggac
    10741 aggtccacca gctcgctctg gatcgccgac gtcggccagg accagcgcga ggagatcgac
    10801 gtcgtcccgg gactcggcgg gccgccgccc aacttcggct ggcccgcctt cgagggcggc
    10861 aacgaccccg acgaccgcaa gccgtccggc cccggccgcc tggtcttccc ggtggccgag
    10921 tacgaccacg acgagggctg ctcggtgacc ggcggctacg tgtaccgggg gagggcggtg
    10981 ccggggctgc gcgggcgcta cgtctacggc gacgtctgca gcggccggct ctggtcgctg
    11041 agggccggac gcgactccgc gaccgacgtt cgccgcgagg acgacgagct cgagcagctc
    11101 tcctcgttcg gcgaggacgc ggccggcgag ctctacgcgg tttcgctcga cggacgcgta
    11161 ttccgactcg gcgcatcgga ttagacgaat gttcgttaga tttccggtac ctagttaggg
    11221 caccctaaga gccatgaccg ccaccgcacc catgatcgag ctcgaccgcg tgaccaaacg
    11281 cttcggcgcg gtcgacgccg tgagcgccac gcggctcacc gtcgagcgcg gcgagctgct
    11341 cgccgtgctc ggcccgagcg gctgcggcaa gaccacgatg ctgcgcctga tcgcgggctt
    11401 cgagttcccc gacggcggca ccgtcaccat cgacgggcgc atcgtgagcg gccgcgactg
    11461 ggtgccgccg gagcgccgcc aggtgggcat ggtgttccag gactacgcgc tcttcccgca
    11521 cctctcggtg gcggagaacg tcggctacgg gctgccgcgc aagcgccgcg ccccccgcgt
    11581 cgccgaggta ctcgcgctgg tcggtctgag cggcctcgag gagcgccgcc cgcacgagct
    11641 ctccggcggc cagcagcagc gcgtcgcgct cgcgcgcgct ctcgcgcccg agccgtccat
    11701 cgtcctgctc gacgagccgt ggagcaacat cgacccgctc ctgcgcggcg ccatgcgcga
    11761 cgacatcgcc cgcatcctgc gcaccgccgg cgtcacggtc gtgctggtca cacacgacca
    11821 ggaggaggcg ttctcgctcg ccgaccgcat cgcgctcatg gacgcgggct cgatcgtgca
    11881 ggtcgggacg ccggaggaga tgtactacca gcccgtctcc cggcgcgtgg ccgagttcgt
    11941 cggcgccacc aacttcgtcg ccgcggagag cccgctcggc cgcgacctcg tgcaggggga
    12001 agcgggcgag gtgctggtcc gccccgagct cgtcggcctc acgatcgacg aggcgggcgg
    12061 cggcaccgtg gtcgggcgcg agttccgcgg ccacgacgtc ttctaccggg tcgagctcgg
    12121 cgacggcggg tcgatctgct cgcagcgtcc ctcgaacgaa gtggtgccgc tgggcgcgcg
    12181 ggtgcgcgtt gagctccacc acggaccggt caaggtcttc tcgtagtgaa gcggctgctg
    12241 gccctggccc tcgccctcac ggcgctggcg ctctccgcct gcggtggcga cgacggcggc
    12301 ggggacggcg tctccggcgg gcccgacaag ctcacgatct actcgggccg caacgaggcg
    12361 ctcatcggcg gcctcttcga ccagttcgag aaggacgcca agatcgacgt cgaggtgcgc
    12421 tacggcgaca gcgccgagct ggcggccacg ctgtccgagg agggcggcaa cagccccgcc
    12481 gacctgttct tctcccagga cgccggcgcg ctcggggccg tcgagaagca gctcaggacg
    12541 ctgcccgccg ccctcgtgaa ccgcgtcgac ccgcgcttcc gcgacgacca gcggcgctgg
    12601 gtcggcgtct cgggccgcgc ccgggtgctc gcctacagca cggagcgcct gaagcccgcc
    12661 gacctcccga agagcgtctt cgacatgacc gacccgcgct ggaaggggaa gctcggcatc
    12721 gcgccgccca acgcctcgtt ccaggcattc gtgagcgcca tgcggatcga cgtcggcgac
    12781 gaccgcgcgc gcaagtttct cgagggcatc aaggccaacg acccgcacct ctacgagaac
    12841 aacctccagg cggtcgaggc gatcgccgac ggcgagatcg acgtggcgct cgtcaaccac
    12901 tactacctct acgagatccg ccgcgagcgc ggcgacgtcc ccgtggacaa ccacttcttc
    12961 cagaccggtg accccggctc gctcgtcaac gcggcgggcg tcggcgtgct gaagtccagc
    13021 gacagcgcct ccgcggcgca gcggctcgtg gactacgtcc tgtcgaagaa ggggcagacc
    13081 tacttcgccg agaagaccgc cgagtacccg ctcgccgcgg gcgtgaagcc cgggcccgag
    13141 ctcaggccgc tcaacagcat ccacggcccg ttcatcgacc tcggcatgct cggcgagaag
    13201 ctgccctcga cgctcgagat gatcgaggag gccgggctca ccaactgatg ccggcggccg
    13261 cggcacccgc cgccccggcg cggcgccggc cgcccgtcgt gctcggactg gtggccgcgg
    13321 cggtggccgc catggtctgc ctgccgctcg tctacctggc gatacgcgcg ggtgggggag
    13381 gggcggacgc ctgggacgtg ctctggcgcg agcgcaccgg tctgctgctc tggcgcacgg
    13441 tcgtcctggt cggtgccgtg accgcggcgg ccgtcgcgat cggcgtgccg ctcgcgtggc
    13501 tcgtgacccg caccgacctg ccgggccggc ggatctgggc cgtggcggcc gcgctcccgc
    13561 tggtgatccc cagctacgtg accgcgctgg cgctcctgtc ggcgttcggc gccggcggcg
    13621 tgatcgagag cgtgcctgac atcaccggct tcacgggctc gttcgccgcg ctcacgctcg
    13681 ccacctaccc atacgtgttc ctgctgagcg cgggggcgct gcgccagctc gacggctcgc
    13741 tcgaggaggc ctcgcgcgcg ctcggccgca cgcgcaccta cacgttcttc cgcgtcacgc
    13801 tgccggtcat ccgcccgtcg gtcgccgccg gtgcgctgct cgttgcgctc tacgcgatct
    13861 ccgacttcgg cg
//
LOCUS       DASVPL010000307        31056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_115296, whole genome shotgun sequence.
ACCESSION   DASVPL010000307 DASVPL010000000
VERSION     DASVPL010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 31056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31056
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115296"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VF517_17560"
     CDS             complement(<1..394)
                     /locus_tag="VF517_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054799.1"
                     /translation="MKRLTLVAAAAAAAVLACAAPAAADNIAYVGADGDVWISSVDGA
                     NKKQVTTDGTPDAAYRQPTQTNDGRILVSKDKFFMLLRQDGSTIHAWQAPTGGSFYKS
                     PGGSQISPDGGLVTWWYYHNTWVYDPIIQ"
     gene            555..1721
                     /locus_tag="VF517_17565"
     CDS             555..1721
                     /locus_tag="VF517_17565"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8054800.1"
                     /translation="MDEAREVIETLAARERGSASEGERWAAHWIADRLTDLGCDAAIE
                     EERVRPDTWRNLIALNAAGALAGLAAARGGRAIPALTATATLAAIADDIQNGLHLARR
                     VTVPELPTWNVTAECGDRDARRTVVVLAHHDAAHSGLVFHQGPQKALWRRRPDVIERS
                     NTAFPIWWPVLAAHALTAAGALTGRRRLARAGAILCGVATAAFADIASRGVVPGANDN
                     LTAVACLAVLARRLREEPVAGVRVLLVSAGSEETFQEGIRAWGRRHFDSLPRESTSFV
                     TIDMVGSPELALIEGEGPIRMEDYDGPLRELVGECAAAESIGLRRKLRARTSTDGVIP
                     MRAGYPTVLLGSVEAWKAPSNYHWPTDTPDRVDYGTVAEASRLVEAVVRRLART"
     gene            1732..2322
                     /locus_tag="VF517_17570"
     CDS             1732..2322
                     /locus_tag="VF517_17570"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054801.1"
                     /translation="MPRTQRQRTEATTADLLDAARLLFAADGYNATSLEDVVAAAGVT
                     KGALYHHFDSKRDLFRAVFENEERALARACHEAYRRKSDPWDGFHAGCTAFLEESLDP
                     GVQRIALLDGPAVLGWETVRTIQAECSLSMIESGLRQAIESKRIAARPTGPLAHMLFG
                     ALCEGAMTVARAGGDQRAAMGEVARELRSLLAGLER"
     gene            2380..4653
                     /locus_tag="VF517_17575"
     CDS             2380..4653
                     /locus_tag="VF517_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054802.1"
                     /translation="MLRGTGTSGRRFGAVAGALASALAIPAVALAHLDHPAPSFVPQA
                     PGSLTAQQGGAGAKWEFLSSFATGNPHTDLDFFRRGDKTYVSVGTLGIGPNGGGQSIF
                     QVMDKGQIAQQYVGSAPTASCVSDPSAATGLQHDVEATPKGGTILNTAWNDADQRDAQ
                     LLVDATDASGRCHDQGAAGITDAPQGGLEIIDITDPAHPVTIGLTSHIGESHTVNIDP
                     RRPHIAYSVTSDNVGVDAQGKRGNESSAEKYDLDGFEVVDLSSCMNFPASATVEQKRA
                     TCRPQVWRYRYPSIAMAQGHTNKTGSNGVFGCHELEVYPDDRLACASGNASILFDLKD
                     TFDDNGTPTNYTDDKVRGNPLPCRVRDSTSVGGFATGAKVTDCVTGAGTAPANDLGVE
                     QWLKNGAPSLAGVKWIGSAFHQGRETTQNSATPDFSSSEDIDFSHEAELSQSGRFLFA
                     TDERGGGIAPPGASCSPGIDNPTGNGGIHAYRVDKLLNRAPTSADDAFTSYAKTPEGN
                     KAIFRAQVRTEPQAALCTAHVFQQIPGQNRIFMGWYSQGTRVIDYTENPDGTISFKEA
                     GYFIPENANQWVSAIYRVDHNNDGSFTYYGTSGDFYVGDGGRGTVDFFKVTLPPPPAP
                     AARLPGTGPGLAPDQFLFNRQALRVSRTGNATLRVSCISASPCKGNMVVRTSQKFRRA
                     DRQNKTFLIAKRSFNVPAGRRDFPLSLRVSSAGRKLAARPRGSSRGAGVQVKAKASVV
                     FGTGRAFIRTKTLRAVR"
     gene            4668..6260
                     /locus_tag="VF517_17580"
     CDS             4668..6260
                     /locus_tag="VF517_17580"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8054803.1"
                     /translation="MVGAGSSGCVLANRLSEDPSVRVLLLEAGGSDRKMNIRVPVGFA
                     KQFRTDLDWNYVSEPEPTLIGRSIYLPRGRSLGGSSSMNAMIYLRGHRSDFDGWAASG
                     APGWSYPEVLPYFKRSEHNERLRDDYHGQGGELNISDPVWLSSLAPFFAESAAGVGIR
                     PNGDFNGVEQEGAGPVQVTQKRGRRWSAADAFLHPVKDRPNLTVATGSHVRRVLLEGG
                     RAVGVQYGEATARAEREVIVSAGAYNSPQVLMLSGIGPGEHLRSVGVEPLVDSPHVGS
                     HLADHPMSTVTYECRDAITLHDATNPRYLLEYLVGRGRGKLSSNVGEAAAHVRVASGA
                     EAPDFQILMGAAYYFDNGFRSYPQPAYTLAPCFIRPLSEGEVRLRSADPHDKPLIRLG
                     WLSDPSEMRAMVDAVRLCREIAETGPLAKATVRNIDPGPGVLSDEQIEAWVRAELQHE
                     YHASCTCRMGRDGDGVLDEELRVRGVDGLRVVDCSSMPRIVAANTNAAAIMLAERASD
                     LILGRATLAAEDPAAEPAAAPA"
     gene            complement(6405..7277)
                     /locus_tag="VF517_17585"
     CDS             complement(6405..7277)
                     /locus_tag="VF517_17585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX8054804.1"
                     /translation="MAPKPAADEGYFPRGRSVLRRVHGERVVGRLYGQRALLLQACHP
                     LAFAGLNANTRGREAPFRRLGHTAKTMETVFFGSRAEADRETERVRTMHAHVRGSIEK
                     PAGPHPAGSEYRADDPEFLLWILACLADSAQATYERFARRLTRPEREAFWQDYLLLGE
                     LFGLDRAHAPANYAAYRGYMAERLASDDLHVTDEAREIGRTVAFDIPLPRQRQPALLA
                     INLAVAGLLPHRVRKLYGIGWSPAHELALEALALSLRRARPLTPRGMRRGPCADEYDL
                     VARTEAARLGRAAA"
     gene            7444..8274
                     /locus_tag="VF517_17590"
     CDS             7444..8274
                     /locus_tag="VF517_17590"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8054805.1"
                     /translation="MPLRRIARATAATLALAAMLAPAAPGAVSGGRSVPSGSATPGRT
                     AAPPSKPAAPPKAPNAPKAPRTDRDTDRPTGPTGGARPGVRRPPAAEDRKDPEPARPA
                     HPPRDEPSERDRDDSDDSSSGANNADIPSEYMRLYRSAGAAEGVSWRLIAAIGKLESD
                     HGRSTLPGVRSGVNGAGCCAGPMQMCTEKSCGNTWQAYARDGDGDGRKSVYAAADAIG
                     AASALLSNLHRMFGEHPAHILAGYNAGPGNVQRYHGVPPFAETQSYVTRGLAYMRGLR
                     "
     gene            complement(8284..8754)
                     /locus_tag="VF517_17595"
     CDS             complement(8284..8754)
                     /locus_tag="VF517_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054806.1"
                     /translation="MHTSRPTRYLTVAAMAIALVGAGCGGDSDEDKAKDYRESLTDAS
                     KKFDQELTQAGTTMRQAGQAKSREQYGQGAEALQEAVDDFKKELEELETPSDAENEEE
                     AVTEAVDDFASSVGRINAAVQAKDDDAVRAEAATVQAKGAEVDQAIDTLKEAVE"
     gene            8853..10775
                     /locus_tag="VF517_17600"
     CDS             8853..10775
                     /locus_tag="VF517_17600"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054807.1"
                     /translation="MPPRQDPEPHHSLRERLRYAFDNTMARGTPALIGWLVVATALLV
                     ALFSIVVLLGDFAPESDDGDRPGVVRQFFNSLLHAMDPGTVAGDDGKWPFLALMLLLT
                     IAGLLIVSALIGVLATGLDRKIEELRKGRSAVLERDHTLVLGWSETVFTVLSELAIAN
                     ENEPNPSVVILADHDRVEMEDAIRAKVPDLRNTRVICRTGSPVDLTDLSVASPQSARS
                     VIILSPEAEGDPDSQVIKAILALTRGPNRRDTPFRIVAEIHDPSNLEAARLVGGDEAV
                     LVDKRETISRLLVQSSRQKGASLVYNDLLDFEGDEVYMRRDPSLDGRTFGDALLAYED
                     CAVIGLRTFGEIALNPPADTEILPGSEIVAIAEDDERLATARPMASPVEEAAIVAAPR
                     RPDQPAAVLILGWNGRTPSVINEFARYLAPGSRVEVLADYAPAREAIGRECSDCGDLE
                     LAVEIGSTTDRRTLDAAGVSEFDHVIVMPYSDLLDHQRADARTLVTLLHLRDIVAGED
                     GHAPTLTSEMLDDRNRELAQVTRVDDVIVSDRILSLMLTQLSENERLAAVFGELFAAE
                     GSEIYLRPAGDYVLLGREVSFATVVEAARRRGEVAVGVHAAAAAEDVHGYDLNPPKSR
                     RITFEATDRVIVLAEE"
     gene            10830..10902
                     /locus_tag="VF517_17605"
     tRNA            10830..10902
                     /locus_tag="VF517_17605"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10863..10865,aa:Thr,seq:ggt)
     gene            10983..11267
                     /locus_tag="VF517_17610"
     CDS             10983..11267
                     /locus_tag="VF517_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054808.1"
                     /translation="MNSERAQAYGRVTKLVDDLSGSKLHATEQQIIRDAADSLFFCED
                     LAADPEARRALDAFEQLVDSMVENERFTPETAGRLQADVEGCGPLVPAAS"
     gene            11328..12635
                     /locus_tag="VF517_17615"
     CDS             11328..12635
                     /locus_tag="VF517_17615"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8054809.1"
                     /translation="MRIAIVGAGPSGLAALRALTNAGFDAVAFERGSRVGGVWTLERR
                     PTAAYRSLHLITSKARTEFAEHPMPAAAPDYPSRDTVGRYIEDYAGAFGLTDRIRLNS
                     AVVDARRADGGGWEVELEGGATERADALVVANGHNEVPKSPDPPYPGEFAGRQLHALD
                     YDDPDELRGRHVMVVGMGNSAMDIVTDASYVAERALLSVRHGSWVIPKRLLGQPADQV
                     VRPWVAVNVPWWIRQPLSEALLRITVGPPDRYGLPKPDGGLFQSHPTITDTVLSRISH
                     GEITPKGPIERFEGDRVRFADGSDERVDTIVWCTGYAVEIPFLDESIVGPDPRELQLY
                     KRVFHLDHDDLFFVGLMQSTGSAFPIVERQSFMLAEHLAGRWALPSRAEMRAECERRR
                     RRALRRWGPHGRPSMRVDFDIYMHELAEEIDAGRQRAAARVAA"
     gene            12632..13435
                     /locus_tag="VF517_17620"
     CDS             12632..13435
                     /locus_tag="VF517_17620"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8054810.1"
                     /translation="MSRRALVTGASGAFGTALRADLRARGWKVAGLDLRPDPGDPEVL
                     ACDVTSDEAVPPAVDAAIARLGGGLDLLVNNAGIGGPASAGEPPSERVRTMLDVNLLG
                     AWRVTAAAIDRLAESRGRVVMIASRMSFMGLPLGAAYGVSKRALVAYADALRAEYGTH
                     VSVTCVHPAYVKTPIHDSTREAGLRLEGFSREEPLERVVAAIVRAAESPRPPRDLAVT
                     RGGSLQIAVARHLPRVVDRAVARTLAKRVAAGEFDGAPVAEGLRARHRR"
     gene            13559..14005
                     /locus_tag="VF517_17625"
     CDS             13559..14005
                     /locus_tag="VF517_17625"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8054811.1"
                     /translation="MLRNLRTRMSDEQGFTLIELLVVILIIGILAAIALPAFLGQQEK
                     GQDADAKSNARNLVSQIESCFATEQTYVSCDTAAELPNTGLSYGTGAGQVQVSASTAS
                     TYTISAVSKAGGATPHTFTIEKTSTGDSVRACTPTGKGGCPNSGAW"
     gene            14123..14590
                     /locus_tag="VF517_17630"
     CDS             14123..14590
                     /locus_tag="VF517_17630"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX8054812.1"
                     /translation="MPQVTVNGIDDLRALMGKEVGPTEWRDVTQDDIDLFARLSGDDQ
                     WIHVDVERAKTESPFGTTVAHGNLTLSMIDGLRRDLIESKGFKLGVNYGWNKVRFPAP
                     VPAGSRVRARAEVTDVDEVGGGWYQVVTKFTVEVEGSEKPACVGESVGRALPG"
     gene            14603..15817
                     /locus_tag="VF517_17635"
     CDS             14603..15817
                     /locus_tag="VF517_17635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8054813.1"
                     /translation="MRTPPESLAALIERWDPEAFDAPGGRARVRLIVKGEGEWDAVVH
                     GAAVTLRDPRGEPDATIAAPVPTWRRIAADPAGGVEEHAAGRLAVRKNMHIGVGFLAA
                     AAGRRDGIGLRFARVKTPNGRMAIQQLGEGPPVLMLHGLGGTKVSFMPTQAALAQTHR
                     TIALDLPGFGDSDKPVGAYDAKFFTRWVVGLMDTLELDRAQVIGHSMGGRVAIELGMR
                     HPDRVECLALMTPALAWLRDKRWTPFLKLLRPELALLQPAPRAMVERFVRSAVPGANG
                     GWSAAGIDEFLRAYLTPRGRMAFYAAARNIYLDESDPFWTRLESVEARSLFIWGRRDQ
                     IIPIGFANHVRRALPAAEHVELDCGHIPQFERPAQTHAAISRFLRGVSTHGGRTRTRT
                     TRAASRTQSGRG"
     gene            15855..16682
                     /locus_tag="VF517_17640"
     CDS             15855..16682
                     /locus_tag="VF517_17640"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8054814.1"
                     /translation="MKALYMKGYEDNVTGLSGMVAYNLLLSLFPLALILLFVAGSILH
                     SPDIEERVLSDLRDLFPDAAESTISDTLTRVRENATSFGVFALLASIWFGSSFWGALD
                     TAFCRIYHVECRKWLEQKRFGLVMLVVIFVFMAATVLLPTLQTTLVRGARNLPFGLAD
                     YGDEVFLVTLAVGLMILFGVLCVIYWAVPNRLVPWRAIWPGALAATAAIGVVDYAFPA
                     YLDESPLTKISSTLLFVMIVLVWFYAIAFIILGGGLINALRFERHDTGRLAIEGRSE"
     gene            16679..18004
                     /gene="rfbH"
                     /locus_tag="VF517_17645"
     CDS             16679..18004
                     /gene="rfbH"
                     /locus_tag="VF517_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HEX8054815.1"
                     /translation="MSDRPREEIRAEILGLVREYHDAEAPGGFVAGESAVPVSGRVYD
                     AEEMVELVDSALDFWLTTGRFAKKLERGLARYVGTRHALLCNSGSSANLLALSALTSP
                     RLGRRRLVDGDEVITVAAGFPTTVNPIFQNRLKPVFVDVDPATGNVVPEALAGAVGPR
                     TRAVMLAHTLGNPFDVDAVRTLCAEHGLWLVEDNCDALGSTYDGQRTGTFGDLATVSF
                     YPAHHITTGEGGCVLVRGGKLKKIVESFRDWGRDCWCDPGDENTCGKRFEWQLGGLPL
                     GYDHKYTYSHVGYNLKMTDLQAAVGVAQLAKLPGFVEARKRNWRRLRDGLAPYADVLM
                     LPEPTPRSDPSWFGFSMCVRPGAPFTRAELLAWLEEAGIATRQVFAGNLTRQPAYRDA
                     DYRIAGELTGTDLVMHQGFWIGVYPGLTDEMVDYMVDAIGRFREQRAAA"
     gene            18113..18277
                     /gene="rpmG"
                     /locus_tag="VF517_17650"
     CDS             18113..18277
                     /gene="rpmG"
                     /locus_tag="VF517_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX8054816.1"
                     /translation="MARGDVRIAVTLACEDCKRRNYQSEKSKRNDPNRIELRKFCRWC
                     GKHTPHKETR"
     gene            18296..18952
                     /gene="secE"
                     /locus_tag="VF517_17655"
     CDS             18296..18952
                     /gene="secE"
                     /locus_tag="VF517_17655"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX8054817.1"
                     /translation="MARDRQKAKQRQEQRRQERLRARGEDPGGQDGAPAPGGDGKVDA
                     DEPAEVGRLAGETGAPPENIGHSDATAESPPPHGVVDEDAIPAGDAGGDPRGRGGPDR
                     ARDLTPDEEDELFLDEEDFEVDQDELAEAEGGEVAYAPRGRRGEKSPASGEVTKAGGG
                     ARVVAFLRACWAELQRVQWPDRKQTTQLTAIVLVFIVIMGGYLGLLDAIVSRVVQEIL
                     "
     gene            18962..19483
                     /gene="nusG"
                     /locus_tag="VF517_17660"
     CDS             18962..19483
                     /gene="nusG"
                     /locus_tag="VF517_17660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX8054818.1"
                     /translation="MFRWYAVNTYSGHENKVKQNLEHRVQSLGQQRNVRSIVIPTEQV
                     SEMKDGQKVQIERRTMPGYVLVNMNLDEDSWTVVKGTPGVTGFVGASQKPVPLSQPEV
                     DRLLNREVAERPRTRAQFSIGESVKVVSGPLSDFSGEISEINEDAAKLKVLVSIFGRE
                     TPVEVGFDQVKKI"
     gene            19546..19968
                     /gene="rplK"
                     /locus_tag="VF517_17665"
     CDS             19546..19968
                     /gene="rplK"
                     /locus_tag="VF517_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX8054819.1"
                     /translation="MAKKVMTLIKLQVPGGAANPAPPVGPALGQHGVNIMEFCKAFNA
                     QTTEEAGRIIPVEITVFEDRSFTFITKTPPAAVLIKEVAGIEKGSGEPNREKVGRITK
                     AQAREIAETKLQDLNASDLDQAAKIIEGTARSMGVEVV"
     gene            20031..20705
                     /gene="rplA"
                     /locus_tag="VF517_17670"
                     /pseudo
     CDS             20031..20705
                     /gene="rplA"
                     /locus_tag="VF517_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
     gene            complement(20807..21625)
                     /locus_tag="VF517_17675"
     CDS             complement(20807..21625)
                     /locus_tag="VF517_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054820.1"
                     /translation="MIRLTRERTEAAVPKGFRGAGRLELEAELLRRRKEGSKPRSAVW
                     KDAKPQLHDESDGKCGYCEGKASHVAHGDVEHYRPKDVYWWLAYCYDNYIYSCQICNQ
                     SYKGINFPAGGTVLTGPAVDPSTSDEEIEALVGTLGPDPLDESSVELHLRASAGEQPG
                     IPDPYALDPEPLFRWNADDVVREVKIEPRDETPAAALAFAAADQYLGLNRDELLRWRY
                     ETYDNARLFVDSLPKLEGDLRARTVEKLNRMMSVHGEFAGMVRYFVRDVWQAPL"
     gene            complement(21643..22917)
                     /locus_tag="VF517_17680"
     CDS             complement(21643..22917)
                     /locus_tag="VF517_17680"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8054821.1"
                     /translation="MIATAESRLFLERIVLENVRSIEHLDLPVTRADGIRRWTFLLGE
                     NGSGKTTLLRSIALALAGSEAMPELLGTPADWVRRGKDEARIIVWLRTAAGEPRHGAL
                     VIHREDTIRDVFSRNVGLLEELDAAFRHTHRSYFTAAYGVSRKPAEDEASVRPKSRFR
                     DPRAQSVASLFWPDYALTPLETWAMDLDYRHPRGYDLVREAIDTLMPGIRLERIDRAN
                     RELIFHTPDGEIPYRLLSDGYQNVAAWAGDLLYQITNVFQDYENPFAARGLLLIDEID
                     LHLHPIWQRGVMRFVEDRFPHLQVVATTHSPLTVHQAGEGELFFLRRRAANAPAQLFS
                     YDGAPRDLMLHQLLTSPVFGLTTLDSRPIEEMKDEYRYLRDLRTRSTAETDRLRELSA
                     ELEDLPDWTDGIQGQDEVKALMRDIRDQLARG"
     gene            23108..24127
                     /gene="rplJ"
                     /locus_tag="VF517_17685"
     CDS             23108..24127
                     /gene="rplJ"
                     /locus_tag="VF517_17685"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX8054822.1"
                     /translation="MNRDEKAAVIDEVAERIEGSQAIFAIDYRGLSVKQAVELRQQLT
                     EAGTHFQVVKNRLTIRAADKAGAEDLKTFLEGPTALAFVQGDAALAAKAIATFRRANN
                     LLEFKGGTLDGDVLSIDQIESIARLPAREVLQGQFVGVLASPITGLVRGLASMISGLA
                     SQLQQIAEQGLVTGEAPAAAADAAPGDDAAPADDAAPAADTAPPEEASAGEPASDASP
                     AGEDAASDDAPSEDAPSDAAPSDAAASDNAPTDDAPTDDAPSGPTPDEMEPAAAAEAS
                     ETVKAEEPDAPAETATEEAPAEEPTPAEEAPGSPTAEAETAEQEETSETSEEPSGAEN
                     QEQEG"
     gene            24128..24511
                     /gene="rplL"
                     /locus_tag="VF517_17690"
                     /pseudo
     CDS             24128..24511
                     /gene="rplL"
                     /locus_tag="VF517_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
     gene            complement(24607..25788)
                     /gene="dinB"
                     /locus_tag="VF517_17695"
     CDS             complement(24607..25788)
                     /gene="dinB"
                     /locus_tag="VF517_17695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX8054823.1"
                     /translation="MSISVLHADLDAFYASVEQRDSPALRGRPVVVGGGVVLACSYEA
                     KARGVRTAMGGSVARRLCPDAVVVPPRMAAYSEASRAVFDVFREFAPHVEGLSIDEAF
                     LDVRGLERITGTAAEIGARLRREVRARVGLPITVGGASTKFLAKVASAVAKPDGLLIV
                     PAGGELAFLHPLPVERLWGVGPVTAGKLHAAGLRSVAQVAAVSEDELVRLLGRGAGRH
                     LHALAHNRDPRRVRTGVRRRSMGAQRALGRWNGSVAELDATVVALADRLARRLRAAGR
                     VCRTVTLRLRFGDYSRATRSHTLAEPTAHTQVILAEGRALLRAALPLVHERGLTLVGL
                     SLGNLADEVPIQLALRPASRADALDAALDDVRDRFGSGAIKRGVLVGRRDDPSVPLLP
                     D"
     gene            25857..26498
                     /locus_tag="VF517_17700"
     CDS             25857..26498
                     /locus_tag="VF517_17700"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX8054824.1"
                     /translation="MSVRVESVNVGEPREVEANGHTVLTAIWKSPVAGRIPVRGVNLR
                     GDDQADRTVHGGPDKAVYAYAAEEIELWAAELGETLGHAPFGENLTVRGLPVSEAVIG
                     ERWSIGTALLEVAQPRLPCFKLGLRMGDPRFPKRFAAADRPGAYLRILREGDIGAGDA
                     IEVQGRPDHGVTCALVARALLREPQLLPAALRAPELPGGLREWMTERAAAHGA"
     gene            26750..30316
                     /locus_tag="VF517_17705"
     CDS             26750..30316
                     /locus_tag="VF517_17705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX8054825.1"
                     /translation="MARVDAPRQRRSFARLETPLELPNLIDIQRRSFEWMVDTEKGGL
                     RETIDDVSPIEDYTGNLAVVFEQIAFDEPTASIDECREKDLTYARPLTVKVAFQNRET
                     GEIREQSVFMGDFPWMTEWGTFIINGTERVVVTQLVRSPGAYVMEPKDREKQVFIANL
                     MPARGSWLELEIDKKGRVYVRIDRKRKLPVTVLLRAMGYVEDQQILDLFDNSLYVRNT
                     LDADTEVTRSEEGALIELFKKQRPGEPPSVDNARALLNTLFFDPKRYDLTRVGRYKLN
                     SRLNLDEDLDTRTLTHRDIIELIKELVSLPRELGIPESGEFDEGEPIKDYALEAQMIP
                     RDPIADRLDEYEHFGNRRLRTVGELIQEAFRIGLYRMERVVRERLTTEDADTITPQTI
                     INIRPVVAALKEFFGSSQLSQFMDQTNSLSGLTHRRRLSALGAGGLTRERAPIEVRDV
                     HPSHYGRMCPIETPEGPNIGLIGSLSSFAEVSEHGFVTTPYRVVEEGVVTDRIVHLDA
                     NEEDEKVIAQANAEFDSKTQKLKGPSVTCRSRDGELTLAAPKDVDLMDVSPEQIWSVA
                     TALIPFLEHDDANRALMGSNMQRQAVPLLKTEAPLIGTGMETRAAIDSGDVLLARRKG
                     TITHVDAERIVIEDGSEKDEYTLHKFMRSNQGTIIHQKPRVSEGQRVKQGDVLADGSS
                     TDQGELALGKNCMVAFMSWEGYNFEDAIIISERLVKDDELTSIHIEEYEIDARTTKLG
                     DEEITRDIPNRSEESLRNLDERGIVRIGAEVGSGDLLVGKVTPKGETELTAEEKLIRA
                     IFKEKAREVRDTSLKVPHGEGGVVIDVLTFRREEGDDLAPGVNELVRVFVAKKRKIAE
                     GDKLAGRHGNKGVISKIVPEADMPHLADGTPVDVILNPLGVPSRMNIGQIMETHLGWV
                     AGEGWYDDGSEAYKQSRNGGDGRVYVATSVFDGASVEQVDEALVRWQEEHHERGVHFE
                     VDKSERPGWRSSGKVQLYNGRTGEPYEETVTVGYMYILKLLHLVDDKIHARSTGPYSL
                     VTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTIKSDDTVGRVKAYEAIVKG
                     ENIAEPSIPESFKVLLKEMQSLALDVNVKSEEGAGVEIRDEDDDLLRAAEELGIDLSG
                     VRAPTAEAADEAEEKIEAEGGGGQTAESPAEQEADYVEDSKE"
     gene            30326..>31056
                     /locus_tag="VF517_17710"
     CDS             30326..>31056
                     /locus_tag="VF517_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054826.1"
                     /translation="MIDINNFDAISIGLASSKQIRSWSSGEVTKPETINYRTLKPEKD
                     GLFCERIFGPTKDWECYCGKYKRVRYKGIVCERCGVEVTRSKVRRERMGHIDLAAPVS
                     HIWFFKGVPSRIGYLLDMAPKELEKILYFAASIVTWVDEEAREKDTAKLEKEVNKVLD
                     AYAAEKEERLLELRESLERREEYLKTGSQTGFIDDDYLWAEALDVDVSKLDEDERQKM
                     LRDLRKTFDSDIADTEAYIEDAAER"
BASE COUNT         4749 a        11286 c        10815 g         4206 t
ORIGIN      
        1 gctggatgat cgggtcgtag acccaggtgt tgtggtagta ccaccacgtc acgagcccgc
       61 cgtccggcga gatctgcgat ccgcccggcg acttgtagaa cgagcccccg gtcggggcct
      121 gccaggcgtg gatcgtcgat ccgtcctggc ggagcagcat gaagaacttg tccttcgaga
      181 cgaggatccg cccgtcgttg gtctgggtcg gctgccggta ggcggcgtcc ggcgtgccgt
      241 cggtcgtgac ctgcttcttg ttggcgccgt cgacggacga gatccacacg tctccgtcgg
      301 cgcccacgta ggcgatgttg tcggcggcgg cgggcgccgc gcaggccagt acggcggctg
      361 cggcggcggc cgccacgagg gtcaggcgct tcatccgggt ctccttgggt gcgtcagggg
      421 taagcatctg ccgccatgct cgcccggaaa gaggcccctc acatcgtgga gacccctaaa
      481 cctgaacccc taaatccatc ccgggggccc taggggggtg tgacccgggt cacgcctcta
      541 acctggcgcg cccggtggac gaggcacgcg aggtcatcga gacgctcgcc gcgcgcgagc
      601 gcggctccgc ctccgagggc gagcgctggg ccgcgcactg gatcgccgac cggctcaccg
      661 acctgggctg cgacgcggcg atcgaggagg agcgcgtgcg gccggacacc tggcgcaacc
      721 tgatcgccct gaacgcggcg ggcgcgctcg ccgggctcgc ggcggcgcgc ggcggccgcg
      781 ccatcccggc cctgaccgcc acggccacgc tcgccgcgat cgccgacgac atccagaacg
      841 gcctccacct tgcccgccgg gtcacggtgc ccgagctgcc cacctggaac gtgaccgcgg
      901 agtgcggcga ccgcgacgcg cgccgcaccg tggtcgtgct cgcccaccac gacgccgcgc
      961 acagcgggct cgtctttcac cagggcccgc agaaggcgct ctggcgccgc cgcccggacg
     1021 tgatcgagcg cagcaacacc gcgttcccga tctggtggcc ggtgctcgcg gcgcacgcac
     1081 tgacggccgc cggcgcgctg accggccgcc gccgcctcgc gcgcgcggga gcgatcctct
     1141 gcggcgtcgc caccgccgcg ttcgccgaca tcgccagccg cggcgtcgtt ccgggggcca
     1201 acgacaacct caccgccgtg gcctgcctcg ccgtgctcgc gcgccgcctg cgcgaggagc
     1261 ccgtagcggg agtgcgcgtt ctgctcgtct cggccgggtc cgaggagacc ttccaggagg
     1321 gcatccgcgc ctggggccgg cgccacttcg actcgctccc ccgcgagagc acgagcttcg
     1381 tgacgatcga catggtcggc tcgcccgagc tcgcgctgat cgagggcgag gggccgatcc
     1441 ggatggagga ctacgacggt ccgctgcgcg agctggtggg ggagtgcgcg gcagcggagt
     1501 cgatcgggct gcgccgcaag ctccgcgcgc gcacgagcac cgacggggtg atcccgatgc
     1561 gggccggcta cccgaccgtg ctgctcggct cggtggaggc atggaaggcg ccgagcaact
     1621 accactggcc gaccgacacg ccggaccgcg tcgactacgg cacggtcgcc gaggcgagtc
     1681 ggcttgtgga ggccgtggtg cgccgcctcg cgcgtaccta acatcccgcc cgtgccgcgg
     1741 acccagcgcc agcgcaccga ggcgaccacg gccgacctgc tcgacgcggc ccgcctcctg
     1801 ttcgcggccg acggctacaa cgcgacctcg ctcgaggacg tcgtggccgc cgcgggcgtg
     1861 accaagggcg cgctctacca ccacttcgac tcgaagcgcg acctgttccg cgcggtcttc
     1921 gagaacgagg agcgagccct ggcgcgtgcg tgccacgagg cctaccgccg caagtccgac
     1981 ccgtgggacg ggttccacgc gggctgcacg gcgttcctcg aggagagcct cgacccgggc
     2041 gtgcagcgga tcgcgctgct cgacggcccc gccgtgcttg gctgggagac cgtgcgcacg
     2101 atccaggcgg agtgctcgct gtcgatgatc gagagcgggc tgcgccaggc gatcgagagc
     2161 aagcggatcg cggcgcggcc gaccggccct ctcgcccaca tgctgttcgg cgcgctctgc
     2221 gagggcgcca tgacggtcgc ccgcgcgggc ggcgaccagc gcgcggcgat gggcgaggtg
     2281 gcgcgcgagc tgcggtccct gctcgccgga ctcgaacgct gatccccccg accgggtagt
     2341 ttgcggtcgc gtggaacccg ggtacaggtc gcgctcagca tgctgagggg gacagggacg
     2401 agtggacgcc ggttcggcgc ggtcgcgggg gcgctcgcct cggctctcgc catccccgcc
     2461 gtcgccctcg cgcacctcga ccaccccgcc ccgagcttcg tgccccaggc cccgggctcg
     2521 ctcaccgcac agcagggcgg cgcgggcgcg aagtgggagt tcctgtcgag cttcgcgacc
     2581 ggcaacccgc acaccgacct cgacttcttc aggcgggggg acaagaccta cgtgtccgtc
     2641 gggacgctcg gcatcggacc gaacggcggc ggccagtcga tcttccaggt gatggacaag
     2701 ggccagatcg cccagcagta cgtgggcagc gcgccgaccg cctcctgcgt cagcgacccg
     2761 agcgccgcga ccggcctcca gcacgacgtc gaggccaccc cgaagggcgg cacgatcctc
     2821 aacaccgcgt ggaacgacgc ggaccagcgc gacgcccagc tcctggtcga cgcgaccgat
     2881 gccagcgggc gctgccacga tcagggcgcc gccggcatca cggacgcgcc ccagggcggc
     2941 ctcgagatca tcgacatcac cgacccggcg caccccgtca cgatcgggct cacgagccac
     3001 atcggcgagt cgcacacggt gaacatcgac ccgcgccgcc cgcacatcgc ctactcggtc
     3061 acctccgaca acgtcggggt ggacgcacag ggcaagcgcg gtaacgagtc ctcggcggag
     3121 aagtacgacc tcgacgggtt cgaggtggtg gacctctcga gctgcatgaa cttccccgcg
     3181 agcgccaccg tcgagcagaa gcgcgcgaca tgccgcccgc aggtctggcg ctaccgctac
     3241 ccctcgatcg cgatggccca ggggcacacc aacaagacgg gttcgaacgg cgtgttcggc
     3301 tgccacgagc tcgaggtcta ccccgacgac cggctcgcct gcgctagcgg caacgcctcg
     3361 atcctgttcg acctgaagga caccttcgac gacaacggga cgcccacgaa ctacaccgac
     3421 gacaaggtgc gcggaaaccc gctgccctgc cgggtccgcg acagcacctc ggtgggcggg
     3481 ttcgccaccg gcgcgaaggt gaccgactgc gtgaccggcg cgggcaccgc tcccgccaac
     3541 gacctcggcg tcgagcagtg gctcaagaac ggggccccct cgctcgcggg cgtgaagtgg
     3601 atcggcagcg cctttcacca gggccgtgag accacgcaga actcggccac tcccgacttc
     3661 tcctcgagcg aggacatcga cttcagccac gaggccgagc tgtcgcagtc gggccgcttc
     3721 ctgttcgcga ccgacgagcg gggcggcggc atcgcgcccc cgggcgcctc gtgctcgccg
     3781 ggcatcgaca acccgaccgg caacggcggc attcacgcct accgggtgga caagctcctg
     3841 aaccgcgcgc cgacgagcgc cgacgacgcg ttcacctcct acgcgaagac gcccgagggg
     3901 aacaaggcga tcttccgcgc gcaggtgcgc acggagccgc aggcggcgct ctgcaccgcg
     3961 cacgtcttcc agcagatccc gggccagaac cgcatcttca tgggctggta ctcgcagggc
     4021 acgcgcgtga tcgactacac ggagaacccc gacgggacca tctccttcaa ggaagccggg
     4081 tacttcatcc cggagaacgc gaaccagtgg gtgtcggcga tctaccgcgt cgatcacaac
     4141 aacgacggct cgttcaccta ctacggcacg agcggcgact tctacgtcgg cgacggcggc
     4201 cgcggcacgg tggacttctt caaggtcacg ctcccgcccc cgccggcacc ggccgcccgg
     4261 cttccgggga ccggccccgg ccttgccccc gaccagttcc tgttcaaccg ccaggcgctg
     4321 cgggtcagcc gcaccggcaa cgccacgctg cgggtctcgt gcatctcggc ctccccgtgc
     4381 aagggcaaca tggtcgtccg gacgagccag aagttccgcc gggcggaccg ccagaacaag
     4441 accttcctga tcgccaagcg cagcttcaac gtgccggcgg gccggcgcga cttcccgctc
     4501 agcctgcggg tgtcgagcgc cggccgcaag ctcgccgcgc ggccgcgggg cagcagccgg
     4561 ggggcgggcg tccaggtgaa ggcgaaggcg tcggtcgtgt tcggtaccgg acgcgccttc
     4621 atccgcacga agacgctgcg cgcggttcgc tgacctacga ctacgtcatc gtcggggcgg
     4681 gctcgtcggg gtgcgtgctc gcgaaccggc tgagcgagga cccgagcgtg cgcgtgctcc
     4741 tgctcgaggc gggcgggtcg gatcgcaaga tgaacatccg cgtgccggtc ggcttcgcca
     4801 agcagttccg caccgacctc gactggaact acgtgtcgga gcccgagccc acgctgatcg
     4861 gccgctcgat ctacctcccc cgcgggcgct cgctcggcgg ctcgagctcg atgaacgcga
     4921 tgatctacct gcgcgggcac cgctcggact tcgacgggtg ggcggcgagc ggcgcgcccg
     4981 gctggagcta ccccgaggtg ctcccgtact tcaagcgctc cgagcacaac gagcgcctgc
     5041 gcgacgacta ccacggccag ggtggcgagc tcaacatctc cgacccggtc tggctcagct
     5101 cgctcgcgcc gttcttcgcc gagtcggcgg cgggggtcgg gatcaggccg aacggcgact
     5161 tcaacggggt cgagcaggag ggcgcggggc cggtgcaggt cacgcagaag cgcgggcggc
     5221 gctggagcgc ggccgacgcg ttcctgcacc cggtcaagga ccgcccgaac ctgaccgtgg
     5281 cgaccggctc gcacgtgcgc cgcgtgctgc tcgagggcgg gcgcgcggtt ggggtccagt
     5341 acggcgaggc cacggcgcgc gccgagcgcg aggtgatcgt ctcggccggc gcctacaact
     5401 cgccccaggt gctgatgctg tcggggatcg gcccggggga gcacctgcgc tcggtcgggg
     5461 tcgagccgct ggtcgactcg ccgcacgtcg gctcccacct ggccgaccac ccgatgagca
     5521 cggtcaccta cgagtgccgc gacgcgatca cgctgcacga cgcgaccaat ccgcgctacc
     5581 tgctcgagta cctggtcggg cgcgggcgcg ggaagctcag ctcgaacgtg ggggaggcgg
     5641 ccgcgcacgt gcgcgtcgcg tcgggggccg aggcgcccga cttccagatc ctcatgggcg
     5701 ccgcgtacta cttcgacaac ggctttcgca gctacccgca gcccgcgtac acgctggcgc
     5761 cgtgcttcat ccggccgctc agcgagggtg aggtgcggct gcgctcggcg gacccgcacg
     5821 acaagccgct gatccggctc ggctggctgt cggacccgag cgagatgcgg gcgatggtgg
     5881 atgccgtgcg gctgtgccgc gagatcgccg agaccggtcc gctcgcgaag gcgaccgtgc
     5941 gcaacatcga tccggggccg ggcgtgctga gcgacgagca gatcgaggcg tgggtgcgcg
     6001 cggagctcca gcacgagtac cacgcgtcgt gcacctgccg gatgggccgc gacggggacg
     6061 gcgtgctgga cgaggagctg cgcgtgcgcg gcgtggacgg cctgcgcgtg gtggactgct
     6121 cgtcgatgcc gcgcatagtc gcggccaaca cgaacgccgc ggcgatcatg ctcgccgagc
     6181 gcgcctccga cctgatcctc ggccgcgcaa cgctggcggc ggaggacccg gcggcggagc
     6241 cggccgccgc gccggcttag cggcccgccc ggcgcgcggt ccgcggcgcg cggctcgtcc
     6301 ggcgcgcgcc cgacccggcg cgcgcccgac ccggcgcgcg cccgacccgg tgcgcgcccg
     6361 acccggcgtg cgcccgaccc ggtgcgcggc tccgcccgcc ccgcctacgc cgcggctcga
     6421 cccaggcgcg ccgcttcggt gcgcgccacc aggtcgtact cgtccgcgca cgggccgcga
     6481 cgcattccgc gcggggtcag cggccgcgct cggcggagcg agagcgccag cgcctcgagc
     6541 gccagctcgt gcgcggggct ccatccgatc ccgtagagct tgcgcacccg gtgcggcagg
     6601 agcccggcca ccgcgaggtt gatcgcgagc agcgccggct ggcgctggcg cggcagcggg
     6661 atgtcgaacg ccacggtccg cccgatctcg cgcgcctcgt cggtcacgtg gaggtcgtcg
     6721 ctcgcgagtc gctcggccat gtagccgcgg taggcggcgt agttggcggg cgcgtgcgcg
     6781 cggtcgagcc cgaagagctc gccgagcagc aggtagtcct gccagaacgc ctcgcgctcg
     6841 ggacgtgtga gccggcgcgc gaaccgctcg taggtcgcct gcgccgagtc ggccaggcag
     6901 gcgaggatcc agagcaggaa ctcaggatcg tccgcgcggt actccgaccc ggccgggtgc
     6961 gggcccgccg gcttctcgat cgacccgcgg acgtgcgcgt gcatcgtgcg gacgcgctcg
     7021 gtctcgcgat ccgcctcggc acggctcccg aagaagaccg tctccatcgt cttcgcggtg
     7081 tgaccgagcc tgcggaacgg agcctcgcgc ccccgcgtgt tcgcgttcag acccgcgaac
     7141 gcgagcgggt ggcacgcctg gagcagcagc gcgcgctgcc cgtagaggcg accgaccact
     7201 cgctcgccgt gcacccgccg cagaaccgac ctcccacgtg ggaagtagcc ctcatcggcg
     7261 gccggttttg gcgccatacc gcgcacccta cccgcgccgg cggcgcgaag gcgcaactgg
     7321 tcgatcctga gcccgcatac ggtgtacaag gtcgaccacc ccccgatgcg gcgcgccgcg
     7381 cgacccgctg caagccgttc gacggggcta acgccgccgc gccccgcgcc gataggacgc
     7441 cggatgccgc tgcgaaggat cgcaagggcc accgccgcca cgctcgcgct cgccgcgatg
     7501 ctcgcccctg ccgcgccggg cgcggtctcg ggcgggcgct ccgtgccgag cggctcggcg
     7561 acacccggcc gcaccgccgc gcccccgtcc aagccggcgg cgccgccgaa ggccccgaac
     7621 gccccgaagg ccccgcgcac cgaccgcgac accgaccgcc ccaccggccc gaccggaggc
     7681 gcgcgccccg gcgtgcgccg cccgcccgcc gccgaggacc gcaaggaccc cgagcccgcg
     7741 cgccccgcgc acccgccgcg cgacgagccg agcgagcgcg atcgcgacga cagcgacgac
     7801 tcctcctccg gcgccaacaa cgccgacatc ccgagcgagt acatgcgcct ctaccgctcc
     7861 gccggcgccg ccgagggcgt gagctggcgg ctaatcgcgg ccatcggcaa gctcgagtcc
     7921 gaccacggcc gctcgacgct cccgggcgtg cgctccggcg tgaacggcgc cggctgctgc
     7981 gccggcccca tgcagatgtg caccgagaag agctgcggca acacgtggca ggcctacgcc
     8041 cgcgacggtg acggcgacgg gcgcaagtcc gtctacgccg ccgccgacgc catcggcgcc
     8101 gcgagcgccc tgctgagcaa cctccaccgg atgttcggcg agcacccggc ccacatcctc
     8161 gcgggctaca acgccggccc gggcaacgtc cagcggtacc acggcgttcc gccgttcgcg
     8221 gagactcagt cctacgtcac gcgcggcctc gcctacatgc gcggcctgcg ctaacgcgcg
     8281 gccctactcc accgcctcct tcagcgtgtc gatcgcctgg tcgacctcgg cgcccttcgc
     8341 ctgcaccgtc gccgcctcgg cgcgcacggc gtcgtcgtcc ttcgcctgaa cggccgcgtt
     8401 gatcctcccg accgaggacg cgaagtcgtc caccgcctcg gtcacggcct cctcctcgtt
     8461 ctcggcgtcg ctcggcgtct ccagctcctc gagttctttc ttgaagtcgt ccaccgcctc
     8521 ctggagcgcc tccgcgccct gcccgtactg ctcgcgcgac ttggcctggc cagcctggcg
     8581 catcgtcgtc cccgcctggg tcagctcctg gtcgaacttc ttggacgcgt cggtgagcga
     8641 ctcgcggtag tccttggcct tgtcctcgtc gctgtcgccc ccgcagccgg ctccgaccag
     8701 cgcgatcgcc atcgcggcga cggtgaggta gcgagtgggg cgggacgtgt gcatgagcaa
     8761 ggttgaacct cctgagcggt gagaaggtgg aaaggatgca tcaccgccag gacggctgca
     8821 tcgccgcgcg cgggtagcct tgcccggcgc ccgtgccgcc ccgccaggat cccgagccac
     8881 atcacagcct gcgcgaacgg cttcgctacg cgttcgacaa cacgatggcg cggggcacgc
     8941 ccgccctgat cgggtggctc gtggtcgcca ccgccctgct cgtcgcgctc ttctcgatcg
     9001 tcgtgctgct cggtgacttc gctcccgaga gcgacgacgg cgaccgcccg ggagtcgtcc
     9061 gccagttctt caacagcctg ctccacgcca tggaccccgg caccgtcgcg ggcgacgacg
     9121 gcaagtggcc gttcctcgcg ctcatgctgc tgctcacgat cgccggcctt ttgatcgtta
     9181 gcgcgctgat cggcgtgctg gccaccggac tcgaccgcaa gatcgaggag ctccgcaagg
     9241 gtcgctcggc ggtgctcgag cgcgaccaca ccctggtgct cggctggtcg gagaccgtgt
     9301 tcacggtcct gagcgagctg gcgatcgcga acgagaacga gccgaacccg tcggtcgtga
     9361 tcctggccga ccacgaccgg gtggagatgg aggacgcgat ccgcgcgaag gtgcccgacc
     9421 tgcgcaacac ccgcgtgatc tgccggaccg gcagccccgt cgacctcacc gacctgagcg
     9481 tggccagccc gcagtcggcg cgctcggtga tcatcctctc gccggaggcc gagggcgacc
     9541 cggactcgca ggtcatcaag gcgatcctcg ccctcacgcg cggtccgaat cgccgcgaca
     9601 cgccgttccg gatcgtcgcc gagatccacg acccgtcgaa cctcgaggcc gcgcggctcg
     9661 tcggcggcga cgaggccgtg ctggtcgaca agcgcgagac catcagccgc ctgctcgtgc
     9721 agtcctcgcg gcagaagggc gcctcgctcg tctacaacga cctgctcgac ttcgagggcg
     9781 acgaggtcta catgcgccgc gacccgtcgc tcgacggccg caccttcggc gacgcgctgc
     9841 tcgcgtacga ggactgcgcg gtgatcggcc tgcggacgtt cggcgagatc gcgctgaacc
     9901 cgcccgccga caccgagatc ctgccgggct ccgagatcgt cgccatcgcg gaggacgacg
     9961 agcggctcgc cacggcgcgg ccgatggcct cgcccgtgga ggaggccgcg atcgtggcgg
    10021 cgccgcggcg cccggaccag cccgcggcgg tgctgatcct cggctggaac ggccgcacgc
    10081 cctcggtgat caacgagttc gcgcgctacc tggcgccggg ctcgagggtc gaggtgctcg
    10141 ccgactacgc gccggcacgc gaggcgatcg gccgcgagtg cagcgactgc ggcgacctcg
    10201 agctcgccgt cgagatcggc agcacgaccg accgccgcac gctcgacgcc gcaggcgtga
    10261 gcgagttcga ccacgtgatc gtgatgccgt actcggacct gctcgaccac cagcgcgccg
    10321 acgcgcgcac gctcgtgacc ctcctgcacc tgcgcgacat cgtcgcgggc gaggacggcc
    10381 acgcgcccac gctcacgagc gagatgctcg acgaccgcaa ccgcgagctc gcacaggtca
    10441 cgcgcgtcga cgacgtgatc gtgagcgacc ggatcctcag cctgatgctc acccagctct
    10501 ccgagaacga gcgcctggcg gcggtgttcg gggagctgtt cgcggccgag gggtcggaga
    10561 tctacctgcg cccggcgggc gactacgtgc tgctcgggcg cgaggtgtcg ttcgcgaccg
    10621 tggtcgaggc ggcgcgccgg cgcggggagg tggccgtggg cgtgcacgcg gcggccgcgg
    10681 cggaggacgt tcacggctac gacctcaacc cgccgaagtc gcgccgcatc acgttcgagg
    10741 cgaccgaccg cgtgatcgtg ctcgccgagg agtagccgct caggccgggc ggcgggcgtg
    10801 ccgatcacgg gctaagctca gcgcgtcacg cccagttagc tcagttggta gagcacttcc
    10861 atggtaagga aggggtcgtc ggttcgagtc cgacactggg ctctcctccc caccgggcgc
    10921 ccccgcgcca gtgatgggtc tcacgtaatc atccgccgct gccgacacta ccctcggtag
    10981 ctatgaactc agagcgcgca caggcatacg gtcgcgttac gaagctggtg gacgacctgt
    11041 ccggcagcaa gctgcacgcg accgagcagc agatcatccg cgacgcggcg gactcgctgt
    11101 tcttctgcga ggacctcgcg gcggaccccg aggcccggcg cgcgctcgac gccttcgagc
    11161 agctcgtcga ctcgatggtc gagaacgagc gcttcacgcc cgagacggcc ggccgcctcc
    11221 aggccgacgt cgagggctgc ggcccgctcg tccccgccgc cagctagcgc caacttacgc
    11281 gagcggccgc ccgcgcgacg cggggggcgg cttaggatgc gcccggcatg cgcatcgcga
    11341 tcgtcggcgc cggaccgtct gggctcgccg ccctgcgcgc gctcacgaac gcgggcttcg
    11401 acgccgtcgc gttcgagcgc ggcagccggg tcggcggcgt ctggacgctc gagcgccggc
    11461 ccaccgccgc gtaccgctcg ctgcacctga tcaccagcaa ggcgcgcacc gagttcgccg
    11521 agcatccgat gcccgccgca gcgccggact acccgagccg cgacaccgtc ggccgctaca
    11581 tcgaggacta cgcgggcgcg ttcggcctca cggaccggat ccggctgaac agcgcggtgg
    11641 tcgacgcgcg ccgcgcggac ggcggcggct gggaggtcga gctcgagggc ggagcgaccg
    11701 agcgcgccga cgcgctggtc gtcgcgaacg ggcacaacga ggtcccgaag tcgcccgacc
    11761 cgccctatcc cggcgagttc gccggccgcc agctccacgc gctcgactac gacgacccgg
    11821 atgagctgcg cggccgccat gtgatggtgg tcggcatggg caacagcgcg atggacatcg
    11881 tcaccgacgc ctcgtacgtg gccgagcgcg ccctgctgtc ggtgcgccac gggagctggg
    11941 tgatccccaa gcgcctgctc ggtcagccgg ccgaccaggt cgtgcgcccg tgggtggcgg
    12001 tgaacgtccc gtggtggatc cgccagccgc tgtcggaggc gctcttgcgc atcacggtcg
    12061 gcccgccgga ccgctacggg ctccccaagc ccgacggcgg tctgttccag tcgcacccga
    12121 cgatcacgga caccgtcctg agccggatca gccacggtga gatcacgccc aaggggccca
    12181 tcgagcgctt cgagggcgac cgcgtgcgct tcgccgacgg cagcgacgag cgcgtggaca
    12241 cgatcgtgtg gtgcaccggc tacgccgtcg agatcccgtt cctggacgag tcgatcgtcg
    12301 gacccgaccc gcgcgagctc cagctctaca agcgcgtgtt ccacctcgac cacgacgacc
    12361 tcttcttcgt cggcctgatg cagtccaccg gcagcgcgtt cccgatcgtc gagcgccagt
    12421 cgttcatgct ggccgagcac ctcgccggtc gctgggcgct cccgtcgcgg gccgagatgc
    12481 gcgccgagtg cgagcggcgg cggcgccggg cgctgcgccg ctggggaccg cacgggcgcc
    12541 cgtcgatgcg cgtcgacttc gacatctaca tgcacgagct ggccgaggag atcgacgccg
    12601 ggcgccagcg cgcggcggcg cgggtggccg cgtgagccgc cgggcgctgg tgaccggcgc
    12661 gagcggcgcg ttcggtaccg ccctgcgcgc cgacctgcgc gcgcgtggat ggaaggtcgc
    12721 gggtctcgac ctgaggcccg acccgggcga ccccgaggtt ctcgcctgcg acgtgacgag
    12781 cgacgaggcg gtgccgcccg cggtcgacgc cgcgatcgcg cgcctcggcg gcgggctcga
    12841 cctgctcgtg aacaacgccg ggatcggcgg tcctgcgagc gccggcgagc cgccgagcga
    12901 gcgcgtgcgc acgatgctcg acgtgaacct gctgggcgcc tggcgcgtga ccgccgccgc
    12961 gatcgaccgg ctggccgagt cccgcgggcg ggtggtgatg atcgcctcgc ggatgtcgtt
    13021 catggggctc ccgctggggg ccgcctacgg cgtgtcgaag cgtgcgctcg tcgcctacgc
    13081 ggacgcgctg cgcgcggagt acggcaccca cgtgtcggtc acctgcgtgc atcccgcgta
    13141 cgtgaagacg ccgatccacg actcgacgcg cgaggccggg ctgcggctcg agggcttctc
    13201 gcgcgaggag ccgctcgagc gcgtggtggc ggcgatcgtc agggcggccg agtcgccgcg
    13261 cccgccgcgc gacctcgccg taacgcgcgg cggctcgctc cagattgcgg tcgcgcggca
    13321 cctcccgcgc gtggtggacc gggccgtcgc gcgcacgctc gccaagcgcg tcgccgcggg
    13381 cgagttcgac ggcgcgccgg tggccgaggg cctgcgcgcc cgccaccggc gctagacatc
    13441 tgtcccattc cgggcgttca cctccgactg ggggctaaag cccctgcacg ttgcgcccga
    13501 tacctggaac cagttagggc cagccctgct ggtcacacca agtcccagga gaaacaacat
    13561 gctgcggaac ctccgtacgc ggatgtcgga cgagcagggc ttcacgctta tcgagcttct
    13621 cgtcgtcatc ctgatcatcg gcatccttgc cgcgattgct ctgccggcct tcctcggcca
    13681 gcaggagaag ggccaggacg cggacgcgaa gtcgaacgcc cgcaacctcg tctcgcagat
    13741 cgagtcgtgc ttcgcgaccg agcagaccta cgtgtcctgt gacacggccg ccgagctgcc
    13801 caacacgggc ctctcgtacg gcactggcgc tggtcaggtc caggtcagcg cctccaccgc
    13861 cagcacctac acgatctcgg ccgtctcgaa ggcgggtggc gccacgccgc acaccttcac
    13921 catcgagaag accagcacgg gcgacagcgt ccgcgcctgc accccgaccg gcaagggcgg
    13981 ctgccccaac agcggcgcct ggtaagaacc gaaaggttca accggcagtt tccgagaggc
    14041 gggcccccgg gcccgcctct cgccttttcc gtgccccctc gcacggctcc ccggcggggc
    14101 agatcgatag cctttgcctc ccgtgcccca ggtgaccgtc aacgggatcg acgacctgcg
    14161 tgccctcatg ggcaaggaag tcggtcccac cgagtggcgc gacgtcacgc aggacgacat
    14221 cgacctcttc gcccgcctgt cgggggacga ccagtggatt cacgtcgacg ttgagcgggc
    14281 caagaccgag agcccgttcg ggacgaccgt cgcgcatggg aacctgacgc tgtcgatgat
    14341 cgacggcctg cgacgtgacc tgatcgagtc gaagggcttc aagctcggcg tcaactacgg
    14401 ctggaacaag gtgcgcttcc ccgcgcccgt gcccgccggg tcgagggtgc gcgcccgcgc
    14461 cgaggtgacc gacgtcgacg aggtcggcgg cggctggtac caggtcgtga cgaagttcac
    14521 cgtcgaggtg gagggctcgg agaagcccgc ctgcgtcggc gagagcgtcg gacgcgcgct
    14581 gccggggtag gacccgcctc ccgtgcgcac ccctcccgag agcctcgccg cgctgatcga
    14641 gcgctgggac cccgaggcct tcgacgcgcc cggcggccgc gcgcgggtgc ggctgatcgt
    14701 gaagggcgag ggcgagtggg acgccgtggt gcacggcgcc gccgtgaccc tgcgcgaccc
    14761 gcgcggcgag cccgacgcga cgatcgccgc cccggtgccc acctggcggc ggatcgccgc
    14821 ggaccccgcc ggcggcgtgg aggagcacgc ggcgggccgg ctggccgtgc gcaagaacat
    14881 gcacatcggc gtgggcttcc tcgcggccgc cgccgggcgc cgcgacggca tcggcctgcg
    14941 cttcgcgcgc gtgaagacgc cgaacgggcg catggcgatc cagcagctcg gcgaggggcc
    15001 gccggtcctg atgctccacg gcctcggcgg cacgaaggtc tcgttcatgc cgacccaggc
    15061 cgcgctggcc cagacccacc ggacgatcgc cctcgacctg cccgggttcg gcgacagcga
    15121 caagccggtg ggtgcctacg acgccaagtt cttcacgcgc tgggtggtcg gcctgatgga
    15181 cacgctcgag ctcgaccgcg cgcaggtgat cggccacagc atgggcggcc gcgtggcgat
    15241 cgagctcggc atgcgccacc ccgaccgcgt cgaatgcctg gcgctgatga cgccggcgct
    15301 cgcctggctg cgcgacaagc gctggacgcc gttcctcaag ctgctgcgcc ccgagctcgc
    15361 gctgctccag cccgcgccgc gcgcgatggt cgagcgcttc gtgcgctccg cggtcccggg
    15421 cgctaacggc ggttggagcg cggcgggcat cgacgagttc ctgcgcgcgt acctcacccc
    15481 tcgcggccgc atggccttct acgccgccgc ccgcaacatc tacctcgacg agagcgaccc
    15541 gttctggacg cggctcgagt ccgtcgaggc gcgctcgctg ttcatctggg gccggcgcga
    15601 ccagatcatc ccgatcggct tcgccaacca cgtgcgccgg gcgctgccgg cggccgagca
    15661 cgtcgagctc gattgcggcc acattccgca gttcgagcgt ccggcccaga cccatgcggc
    15721 gatcagccgc ttcctgcgcg gggtatccac ccacggtgga cgcacacgca cgcgaacaac
    15781 ccgcgccgcc agccggacgc agtccgggcg cggctagcga ggcgcgccgc agggcgcgcg
    15841 gggcctggcg ctacgtgaag gccctctaca tgaagggcta cgaggacaac gtcacgggcc
    15901 tgtccgggat ggtcgcctac aacctgctgc tctcgctgtt cccgctcgcg ctgatcctgc
    15961 tgttcgtggc gggcagcatc ctgcacagcc ccgacatcga ggagcgcgtt ctctccgacc
    16021 tgcgcgacct gttccccgac gccgccgaga gcacgatctc ggacacgctc acgcgcgtgc
    16081 gcgagaacgc cacgagcttc ggcgtgttcg cgctgctcgc gagcatctgg ttcgggtcgt
    16141 cgttctgggg cgcgctcgac accgcgttct gccggatcta ccacgtcgag tgccgcaagt
    16201 ggctcgagca gaagcggttc ggcctcgtga tgctcgttgt gatcttcgtc ttcatggccg
    16261 cgacggtgtt gctgcccacg ctccagacca ccctggtccg cggcgctcgg aacctgccgt
    16321 tcgggctcgc cgactacggc gacgaggtct tcctcgtaac gctcgccgta gggctgatga
    16381 tcctgttcgg cgtgctgtgc gtgatctact gggccgtgcc gaaccgcctc gtgccgtggc
    16441 gggcgatctg gcctggcgcg ctggccgcca ccgcggcgat cggcgtggtc gactacgcgt
    16501 tccccgccta cctcgacgag tcgccgctca cgaagatctc gtccacgctg ctgttcgtga
    16561 tgatcgtgct ggtctggttc tacgcgatcg cgttcatcat cctcgggggc gggctgatca
    16621 acgccctgcg cttcgagcgc cacgacacgg ggcgcctcgc gatcgagggg aggagcgagt
    16681 gagcgaccgc ccgcgcgagg agatcagggc ggagatcctc gggctggtac gcgagtacca
    16741 cgacgccgag gcgcccggcg ggttcgtggc cggggagtcg gcggtgcccg tgtcggggcg
    16801 cgtctacgac gccgaggaga tggtcgagct cgtggactcg gcgctcgact tctggctcac
    16861 cacgggacgc ttcgcgaaga agctcgagcg cgggctggcg cgctacgtgg gcacgcgcca
    16921 cgcgctgctc tgcaactcgg gctcctcggc gaacctgctc gccctgtcgg cgctcacctc
    16981 gccgcgcctc ggccggcgcc ggctcgtgga cggcgacgag gtgatcaccg tcgcggccgg
    17041 gttcccgacc accgtcaacc cgatcttcca gaaccgcctc aagccggtgt tcgtggacgt
    17101 ggatcccgcc accggcaacg tcgtgccgga ggcgctggcg ggtgcggtcg gcccgcgcac
    17161 ccgcgcggtc atgctcgcgc acacgctcgg caacccgttc gacgtcgacg ccgtgcggac
    17221 cctctgcgcc gagcacggcc tctggctcgt cgaggacaac tgcgacgcgc tcggctccac
    17281 ctacgacggt cagcgcaccg gcaccttcgg cgacctcgcg acggtctcgt tctaccccgc
    17341 ccaccacatc acgaccggcg agggcggctg cgtgctcgtg cgcggcggca agctcaagaa
    17401 gatcgtcgag tcgttccgcg actgggggcg cgactgctgg tgcgaccctg gtgacgagaa
    17461 cacctgcggg aagcggttcg agtggcagct cggcggcctg cccctcggct acgaccacaa
    17521 atacacgtac tcgcacgtgg gctacaacct gaagatgacc gacctccagg cggcggtcgg
    17581 cgtggcgcag ctcgcgaagc tgcccgggtt cgtggaggcg cgcaagcgca actggcgccg
    17641 gctgcgcgac gggctcgcgc cctacgcgga cgtgctgatg ctcccggagc cgaccccccg
    17701 cagcgacccg agctggttcg gcttctcgat gtgcgtgcgc cccggcgcgc cgttcacccg
    17761 cgccgagctg ctggcctggc tcgaggaggc cggcatcgcc acgcggcagg tcttcgcggg
    17821 caacctcacg cgtcagcccg cctaccggga cgccgactac cggatcgcgg gcgagctgac
    17881 cggaaccgac ctcgtcatgc accaggggtt ctggatcggc gtctacccgg gcctgaccga
    17941 cgagatggtc gactacatgg tcgacgcgat cgggcgcttc cgggagcagc gggccgcggc
    18001 ctagcgccag ctttcagcgc ctgcgcgccg ctacactgcg gcacttgacc tcgcggcccc
    18061 tgcggggtcg ttcttcgcag ggggccgtcc accgtttgac cgtcagggag ccatggcccg
    18121 cggagacgtc cgaatcgccg tgacgctcgc ttgcgaggac tgcaagcggc gcaactatca
    18181 gtcagagaag tcgaagcgca acgatcccaa ccggatcgag ctgcgcaagt tctgccgctg
    18241 gtgcgggaag cacaccccgc acaaggaaac gcgctaggag ccgggccctc gtctgatggc
    18301 tagggaccgc caaaaggcga agcagcgtca ggagcagcgg cgccaggagc gtctgcgcgc
    18361 gcgcggcgag gacccgggcg gccaggacgg cgccccggcc cccggcggcg acggcaaggt
    18421 cgacgccgac gagcccgccg aggtcggccg cctagcgggc gagaccggcg cgccgcccga
    18481 gaacatcggc cacagcgacg cgaccgccga gtcgccgcct ccgcacgggg tcgtcgacga
    18541 ggacgcgatc cccgcgggcg acgccggcgg cgacccccgc ggccgcggag gcccggaccg
    18601 cgctcgcgac ctgacccccg acgaggagga cgagctcttc ctcgacgagg aggacttcga
    18661 ggtagaccag gacgagctgg ccgaggccga gggcggcgag gtggcctacg cgccccgcgg
    18721 ccgccgcggc gagaagtccc cggcgtcggg tgaggtcacc aaggcgggcg gtggcgctag
    18781 ggttgtcgcg ttcctgcgcg cctgctgggc cgagctccag cgtgtgcagt ggccagatcg
    18841 caagcagacc actcagctga ctgcgatcgt cctcgtcttc atcgtcatca tgggcggcta
    18901 cctggggctg ctcgacgcga tcgtctcgcg ggtcgtccag gagatcctct agagagagcc
    18961 aatgttccgc tggtacgccg tcaacaccta ttcagggcac gagaacaagg tcaagcagaa
    19021 cctcgagcat cgtgtccagt cgctcggtca gcagcgcaac gtgcgctcga tcgtcatccc
    19081 caccgagcag gtctcggaga tgaaggacgg tcagaaggtc cagatcgagc gccgcacgat
    19141 gcccggctac gtgctcgtga acatgaacct ggacgaggac tcgtggaccg tcgtgaaggg
    19201 cacgcccggc gtgaccggct tcgtgggggc gtcgcagaag cccgtgccgc tgagccagcc
    19261 cgaggtcgac cggctgctca accgcgaggt cgccgagcgc ccgcgcacgc gcgctcagtt
    19321 ctcgatcggg gagtcggtca aggtcgtctc cggtccgctc tcggacttct ccggtgagat
    19381 ctccgagatc aacgaggacg ccgccaagct caaggtgctg gtctcgatct tcggccgcga
    19441 gactcccgtc gaggtcggct tcgaccaggt gaaaaagatc tgagacgtga agtcgaagac
    19501 caacatcaga acgcggggct ggaagccccg cggaggtaat cgctagtggc caagaaggtc
    19561 atgacactca tcaagctcca ggtccccggc ggggccgcga atcccgcgcc gccggtcgga
    19621 cccgcgctcg gtcagcacgg cgtgaacatc atggagttct gcaaggcgtt caacgcgcag
    19681 acgaccgagg aggccgggcg catcatcccg gtcgagatca cggtcttcga ggaccgctcg
    19741 ttcaccttca tcacgaagac gccgccggcg gcggtgctca tcaaggaggt cgcggggatc
    19801 gagaagggct ccggcgagcc gaaccgcgag aaggtcgggc gcatcaccaa ggcgcaggcg
    19861 cgcgagatcg cggagaccaa gcttcaggac ctgaacgcgt ccgacctcga ccaggcggcg
    19921 aagatcatcg agggcaccgc gcgctcgatg ggcgtggagg ttgtttagag aggcgtgatg
    19981 ccgaaccacg acatcaagac gcgggcgtgg atgcctcgcg gaggtaacta atggcgaagc
    20041 atggcaagcg ctaccgcgag ctctactccc gcgtcgaccg cgagcgcgcc tacacgcccg
    20101 ccgaggcggt gcagctcgtg aagagccttc agtccacgaa ggccaccgag accgtcgaga
    20161 tccacgtgcg caccgggctc aacgtgcgcc acgcggagga gcagctccgc ggcacgatca
    20221 cgcttccgaa cagcgagggc acggacgtga ccgtggccgt gttcgcgcgc ggcgacaagg
    20281 cgcgtgaggc cgaggccgcc ggcgccgacg tcgtcggcga cgaggacctc gccaagcggg
    20341 tcgaggaagg ctggaccgac ttcgacgtgg cgatcgcgac gccggacatg atgccggtgg
    20401 tgggtcgcct cggccgggtt ctcgggccgc agggcaagat gccgaacccg aagctcggca
    20461 ccgtcaccca ggacgtgggg caggccgtga ccgaggccaa ggccggcaag gtcgaatacc
    20521 gcaccgaccg ctcggcgatc gtccacctgg cgatcggcaa gacctcgctc gacgagcgcc
    20581 agctcctcga gaactacgcc gcgctgatcg acgagctcgt ccgcgcgaag cccgccgccg
    20641 ccaaggggcg ctacctgcgc acgatcacgc tcgccacgag ccactcgccg ggcatccgcg
    20701 tggacccggc gcgcacgcgc gacatcctcg aggaggccat ccccggcgat ggcgcctcgg
    20761 agaacggagc cggcgacgac gccgccgctc cggtcgacgc caccgcctag agcggtgcct
    20821 gccagacgtc cctgacgaag taccggacca ttccggcgaa ctcgccgtgg acggacatca
    20881 tcctgttgag cttctcgacg gtcctggccc ggaggtcgcc ctccagcttc gggagggagt
    20941 cgacgaacag gcgggcattg tcgtaggtct cgtaacgcca gcgcagcagc tcgtcgcggt
    21001 tgagcccgag gtactggtca gcggccgcga acgccagcgc tgcagccggc gtctcgtctc
    21061 gcggctcgat cttgacctct ctcaccacgt cgtctgcgtt ccacctgaag agcggctccg
    21121 ggtcgagcgc atacgggtcg gggatcccgg gctgctcacc cgccgacgcc cgcaggtgca
    21181 gctcgacgga ggactcgtcg agcgggtcgg gacccagcgt gccgacgagc gcctcgatct
    21241 cctcgtcgga ggtcgacggg tctacggccg gccccgtcag aacagttccg ccggctggga
    21301 agttgatgcc cttgtagctc tggttgcaga tctggcagga gtagatgtag ttgtcgtagc
    21361 agtacgcaag ccaccagtag acgtccttcg gccggtagtg ctccacgtcg ccgtgggcca
    21421 cgtggctggc cttgccctcg cagtagccgc acttgccgtc ggactcgtcg tgaagctggg
    21481 gcttggcgtc cttccagacc gccgatcgcg gcttgctccc ctccttgcgc cgccggagca
    21541 gctcggcctc gagctcgagt cgccccgctc cgcggaagcc cttcggaacc gccgcctcgg
    21601 tccgctctct ggtcagtcgg atcacgtcgg tctacgagat ggtcagcccc tggcgagctg
    21661 gtccctgatg tcacgcatca gggccttcac ctcgtcctgt ccctggatcc cgtccgtcca
    21721 gtcgggcaga tcctcgagct ccgccgagag ctccctgagg cgatccgtct cggcggtgct
    21781 gcgcgtgcgc aggtcccgca agtatcggta ctcgtccttc atctcctcga tcggccgtga
    21841 gtcgagcgtc gtcaggccga acaccggact ggtcaggagc tgatggagca tcaggtcacg
    21901 gggcgcgccg tcgtacgaga agagctgcgc gggtgcgttg gcggcgcgcc tccggaggaa
    21961 gaacagctca ccctcgcccg cttggtggac cgtgagcggg ctgtgcgtcg tcgcgaccac
    22021 ctggaggtgg gggaagcggt cctccacgaa ccgcatcacc ccgcgttgcc agatcggatg
    22081 gaggtggagg tcgatctcgt ctatgagcag gagaccgcgc gcggcgaatg ggttctcgta
    22141 gtcctggaag acgttggtga tctggtagag caggtccccc gcccaggcgg cgacgttctg
    22201 gtacccgtcg ctcagcaggc ggtaggggat ctcgccgtcc ggcgtatgga agatgagctc
    22261 gcgattcgcc cgatcgatcc gctccagcct gatccccggc atgagcgtgt cgatcgcctc
    22321 ccggacgagg tcgtagccgc gcgggtggcg gtagtcgagg tccatcgccc acgtctccag
    22381 cggggtcagc gcgtaatccg gccagaacag ggaggccacg ctctgcgcgc gcgggtcgcg
    22441 gaagcgagac ttgggccgca cgctcgcctc gtcctcggcc ggcttgcgcg acacgccgta
    22501 ggcggcagtg aagtagctgc ggtgcgtgtg tctgaaggcg gcgtcgagct cctcgagcag
    22561 cccgacgttc cggctgaaca cgtcacggat cgtgtcctca cggtggatga cgagcgcgcc
    22621 gtgacgcggt tcgccggcgg cggtcctgag ccacacgatg atgcgcgctt cgtccttgcc
    22681 tcgccggacc cagtcggcgg gggtgccgag cagctccggc atggcttcgc ttccggccag
    22741 tgcgagcgct atcgagcgca gcagcgtcgt cttgccgctg ccgttctcgc cgaggaggaa
    22801 ggtccagcgc ctgatcccgt ccgcgcgcgt gaccggcaga tcgaggtgct cgatcgatct
    22861 gacgttctct aggacgatcc gctcgaggaa caaacgcgac tccgctgtcg cgatcatacg
    22921 cctccggacc ccggccgccc gaccgcgcct cgccgctcgc gcgggcgccc gctaccctca
    22981 ccgccgcaca accgagctgc cccagacccc cggcaggcgc gagccgtaag acctgggcag
    23041 gcgattccgt cggagaccgt tcctcggagc ccgcctgcgt gcgggctttg tctttggaga
    23101 cgaccgagtg aacagagatg agaaggccgc ggtcatcgac gaggtggccg agcggatcga
    23161 gggctcgcag gcgatcttcg cgatcgacta tcgcgggctg tcggtcaagc aggcggtcga
    23221 gctgcgccag cagctcaccg aggccgggac gcacttccag gtggtcaaga accgcctcac
    23281 gatccgcgcc gccgacaagg cgggcgccga ggatctgaag accttcctcg agggcccgac
    23341 cgcgctcgcg ttcgtgcagg gcgacgcggc actggcggcc aaggccatcg cgaccttccg
    23401 ccgggccaac aacctgctcg agttcaaggg cggcacgctc gacggcgacg tcctgtcgat
    23461 agaccagatc gagtcgatcg cgcggcttcc ggcccgcgaa gtgctccagg gccagttcgt
    23521 cggcgtgctc gcctcgccga tcaccggcct ggtgcgcggc ctggcctcga tgatctcggg
    23581 gctggcgagc cagctccagc agatcgccga gcaggggctg gtgacgggcg aggccccggc
    23641 ggcggccgcc gatgcggccc ccggggacga cgcggctccg gcggacgacg cggctccggc
    23701 cgccgacacg gccccccccg aggaggcttc cgccggcgag ccggcttcgg acgcttcccc
    23761 cgctggggag gacgccgctt ccgacgacgc cccttcggag gacgctcctt cggacgccgc
    23821 tccttcggac gccgctgctt cggacaacgc ccctacggac gacgccccta cggacgacgc
    23881 cccttcgggc cccacgcccg acgagatgga gccggccgcg gcggcggagg cttcggagac
    23941 ggtgaaggcc gaggagccgg acgctcccgc cgagaccgcg acggaggagg ctcccgccga
    24001 ggaacccacc cccgcggagg aggcgcccgg ctcgccgacc gccgaggccg agactgccga
    24061 gcaggaagag acatcggaga cttcggagga gccctccgga gccgagaacc aggaacagga
    24121 gggctagatg cctacgacac aggaatggat cgacgagctg aagggcattt cggtgctcga
    24181 gctcagtgag cggatctagg cgctcgagga ggagttcggc gtatcggcga ccgcggtggc
    24241 cgccgcggcc ccggccggcg gcggagccgc cgccgacggc gctgccgagg aggaggtctc
    24301 gaccacggtc gacgtcgtgc tcaccggctc cggcgacaag aagatccagg tcatcaaggt
    24361 cgtccgcgcg gcgaccggcc tcggcctcaa ggaggccaag gcgctcgtgg acgaggcccc
    24421 caagccggtc aaggagggca tcgaccgcga ggaggccgag aagctgaagg gcgagatcga
    24481 ggaggccggc ggcagcgtcg agctgaagta gcgcccgagc ggccgcggcc gcgatgagtc
    24541 ttccttcaac cgtcctgtcc tagggggaca ccgtcccctc aaggcaggac ggtcgtcgtt
    24601 ttcgcctcag tccggcagca gtgggaccga ggggtcgtcg cggcggccga cgagcacccc
    24661 tcgcttgatc gcgccgctgc cgaagcggtc gcgcacgtcg tcgagggccg cgtcgagggc
    24721 gtccgcgcgg gaggccggcc gcagcgccag ttggatcggc acctcgtcgg ccaggttccc
    24781 gagcgagagc cccacgagcg tcaacccgcg ctcgtgcacc agcggcagcg ccgcgcgcag
    24841 cagcgcccgc ccctcggcga ggatcacctg cgtgtgcgcg gtcggctcgg cgagcgtgtg
    24901 cgagcgggtc gcccgcgagt agtcgccgaa gcgcagccgc aacgtgaccg tgcggcagac
    24961 gcggcccgcc gcccgaagcc ggcgcgcgag ccggtccgcg agagccacca ccgtcgcgtc
    25021 cagctccgcg accgagccgt tccagcgccc aagtgcccgc tgcgccccca tggagcggcg
    25081 acgaacgccg gtccgcactc gtcgcgggtc gcggttgtgc gcaagcgcgt gcaggtgccg
    25141 tcccgccccg cgccccagca gcctcacgag ctcgtcctcc gacacggccg ccacctgagc
    25201 cacgctccgc agtccggccg cgtggagctt gcccgccgtc accggcccca ctccccagag
    25261 ccgttcgacg ggcagcgggt ggaggaacgc gagctcgcct cccgccggca cgatcagcag
    25321 accgtccggc ttcgcgacgg cgctcgccac cttcgcgagg aacttggtgg acgcgccgcc
    25381 gaccgtgatc ggcagtccca cccgcgcccg cacctcacgc cgcagccgcg ccccgatctc
    25441 cgcggccgtg cccgtgatcc gctccaaccc gcgcacgtcg aggaacgcct cgtcgatcga
    25501 cagcccctcc acgtgcggcg cgaactcgcg gaacacgtcg aacaccgccc ggctcgcctc
    25561 cgagtaggcc gccatccgcg gcggcacgac cacggcatcg gggcagagcc ggcgagcgac
    25621 ggagccgccc atcgcggtcc gcaccccgcg cgccttcgcc tcgtacgagc aggccagcac
    25681 cacacctccg cccacgacga ccggccgccc gcgcagcgcg ggcgagtcgc gctgctcgac
    25741 cgacgcgtag aacgcatcga gatcggcgtg gagaacggag attgacacga acacatgttc
    25801 gcattcggcg acgacgcgtt gcaacgccgc ccgcaccttc gccagactca accgcgatgt
    25861 ccgtaagggt cgagtccgtc aacgtcggcg agccgcgcga ggtcgaggca aacggccaca
    25921 cggtcctcac ggcgatctgg aagagcccgg tcgcggggcg gatccccgtg cgcggcgtga
    25981 atctgcgcgg cgacgaccag gccgaccgga ccgtgcacgg cggacccgac aaggccgtct
    26041 acgcgtacgc cgccgaggag atcgagctgt gggcggccga gctgggggag acgctcggcc
    26101 acgctccgtt cggcgagaac ctgacggtgc gcggcctccc ggtctcggag gcggtgatcg
    26161 gcgagcgctg gagcatcggc accgcgctgc tcgaggtcgc ccagccgcgc ctgccgtgct
    26221 tcaagctcgg cctgcggatg ggcgacccac ggtttccgaa gcgcttcgcc gccgcggaca
    26281 ggcccggcgc gtacctccgc atcctccgcg agggcgatat cggagccggc gacgcgatcg
    26341 aggtgcaggg ccgcccggac cacggcgtca cctgcgcgct cgtcgcccgg gcgctcctgc
    26401 gcgagccgca gctcctcccg gccgcgctca gagctcccga gctcccgggc ggcctgcgcg
    26461 agtggatgac cgagcgcgcc gccgcgcacg gcgcctagca ccagcaagcc cccgcgcggt
    26521 ggtttttcgc cgctcacccg ggtaacatcg ccgcaggcca atagccgcag ccgtccctca
    26581 gtagctgctc cgcccacata tcgcggagcc tcgggacggg gtccgtgacg gccgctcagc
    26641 gccccgaaag gcggcgctgt tcgtcgtgct atattgaccg gtcgcgtcag cgcgccccag
    26701 cgcgcagcgt ccagccctca cctccgtccg acctcaagga gccgccgtct tggcccgtgt
    26761 tgatgccccc cgtcagcgcc gcagttttgc gcgcctcgaa acacccctcg agcttccgaa
    26821 cctgatcgac atccagcgcc gctcgttcga gtggatggtg gacaccgaga aggggggcct
    26881 gcgcgagacc atcgacgacg tctccccgat cgaggactac accggcaacc tcgccgtcgt
    26941 cttcgagcag atcgccttcg acgagcccac cgcctcgatc gacgagtgcc gtgagaagga
    27001 cctcacctac gcgcgcccgc tgaccgtcaa ggtcgccttc cagaaccgcg agaccggcga
    27061 gatccgcgag cagtccgtct tcatgggcga cttcccctgg atgaccgagt ggggcacgtt
    27121 catcatcaac gggaccgagc gcgtggtcgt cacgcagctc gtccgctcgc ccggcgccta
    27181 cgtcatggag ccgaaggacc gcgagaagca ggtcttcatc gcgaacctga tgccggcccg
    27241 cggctcgtgg ctcgagctcg agatcgacaa gaagggccgc gtgtacgtcc ggatcgaccg
    27301 caagcgcaag ctgccggtca cggtcctcct gcgcgcgatg ggctacgtcg aggaccagca
    27361 gattctcgac ctgttcgaca actcgctcta cgtccggaac acgctcgacg ccgacaccga
    27421 ggtcacccgc tccgaggagg gcgcgctgat cgagctgttc aagaagcagc gccccggcga
    27481 gccgccgtcg gtcgacaacg cccgcgcgct cctgaacacg ctgttcttcg acccgaagcg
    27541 ttacgacctc acgcgcgtcg gccgctacaa gctgaacagc cgcctgaacc tcgacgagga
    27601 cctcgacacc cgcacgctca cgcaccggga catcatcgag ctcatcaagg agctcgtctc
    27661 gctgcccagg gagctgggca tccccgagtc cggcgagttc gacgagggcg agccgatcaa
    27721 ggactacgcc ctcgaggcgc agatgatccc gcgcgacccg atcgccgacc ggctcgacga
    27781 gtacgagcac ttcggcaacc gccgcctgcg caccgtcggc gagctgatcc aggaggcgtt
    27841 ccggatcggc ctctaccgca tggagcgcgt ggtccgcgag cgcctcacca ccgaggacgc
    27901 ggacacgatc acgccgcaga cgatcatcaa catccgcccg gtcgtggccg cgctgaagga
    27961 gttcttcggc tcctcgcagc tctcgcagtt catggaccag acgaactcgc tgtccggcct
    28021 gacgcaccgc aggcgcctca gcgccctcgg cgccggcggc ctcacccgcg agcgcgcgcc
    28081 catcgaggtc cgcgacgtgc acccgagcca ctacggccgc atgtgcccga tcgagacgcc
    28141 ggagggcccg aacatcggcc tgatcggctc gctctcgagc ttcgccgagg tctccgagca
    28201 cggcttcgtc accacgccct accgggtggt cgaggagggc gtggtcaccg accggatcgt
    28261 ccacctcgac gccaacgagg aggacgagaa ggtcatcgcc caggcgaacg ccgagttcga
    28321 ctcgaagacg cagaagctca agggcccgag cgtcacctgc cgttcacgcg acggcgagct
    28381 gaccctcgcc gcgccgaagg acgtcgacct gatggacgtc tcgcccgagc agatctggtc
    28441 ggttgccacg gcgctgatcc cgttcctcga gcacgacgac gccaaccgcg cgctgatggg
    28501 ctcgaacatg cagcgccagg ccgttccgct gctcaagacc gaggcgccgc tgatcggcac
    28561 cgggatggag acccgtgccg cgatcgactc cggcgatgtc ctgctagccc gccgcaaggg
    28621 caccatcacg cacgtggacg ccgagcggat cgtcatcgag gacggctccg agaaggacga
    28681 gtacacgctc cacaagttca tgcgctcgaa ccagggcacg atcatccacc agaagccgcg
    28741 cgtgtccgag gggcagcggg tcaagcaggg tgacgtgctc gcggacgggt cctcgaccga
    28801 ccagggcgag ctggcactcg gcaagaactg catggtcgcc ttcatgtcct gggagggcta
    28861 caacttcgag gacgcgatca tcatctcgga gcggctggtc aaggacgacg agctcacctc
    28921 gatccacatc gaggagtacg agatcgacgc ccgcaccacc aagctgggcg acgaggagat
    28981 cacgcgcgac atcccgaacc gctcggagga gtcgctccgc aacctcgacg agcgcggcat
    29041 cgtccgcatc ggcgccgagg tcggctcggg cgacctgctg gtcggcaagg tcacgccgaa
    29101 gggcgagacc gagctcaccg ccgaggagaa gctgatccgc gcgatcttca aggagaaggc
    29161 gcgcgaggtc cgcgacacat cgctcaaggt gccccacggc gagggcggcg tcgtcatcga
    29221 cgtgctcacc ttccgccgcg aggagggcga cgacctcgcc cccggcgtca acgagctcgt
    29281 ccgcgtgttc gtggcgaaga agcgcaagat cgccgagggc gacaagctcg cgggccgcca
    29341 cggcaacaag ggcgtgatct cgaagatcgt gcccgaggcc gacatgccgc accttgcgga
    29401 cggcaccccg gtcgacgtga tcctcaaccc gctcggcgtg ccgagccgga tgaacatcgg
    29461 ccagatcatg gagacgcacc tcgggtgggt cgccggcgag ggctggtacg acgacggctc
    29521 cgaggcctac aagcagtcgc ggaacggtgg cgacggtcgc gtgtacgtgg ccacgtcggt
    29581 gttcgacggc gcgtcggtcg agcaggtgga cgaggcactc gtgcgctggc aggaggagca
    29641 ccacgagcgc ggcgtccact tcgaggtcga caagtcggag cggcccgggt ggcgctcgag
    29701 cggcaaggtc cagctctaca acgggcggac cggcgagccg tacgaggaga ccgtcacggt
    29761 cggctacatg tacatcctga agctgctcca cctcgtggac gacaagatcc acgcacggag
    29821 cacgggcccc tactcgctcg tcacccagca gccgctgggc ggcaaggcgc agttcggcgg
    29881 ccagcgcttc ggcgagatgg aggtgtgggc gctcgaggcg tacggcgcgg cgtacacgct
    29941 ccaggagatg ctcacgatca agtccgacga caccgtcggg cgcgtgaagg cgtacgaggc
    30001 gatcgtcaag ggcgagaaca tcgccgagcc gtcgatcccc gagtccttca aggtgctgct
    30061 gaaggagatg cagtcgctgg cgctcgacgt caacgtcaag tcggaggagg gagcaggcgt
    30121 cgagatccgc gacgaggacg acgacctgct gcgcgccgcg gaggagctgg gcatcgacct
    30181 gtccggcgtc cgcgcgccca ccgcggaggc ggccgacgag gccgaggaga agatcgaggc
    30241 cgagggcggt ggcgggcaga ccgccgagtc gccggccgaa caggaagctg actacgtcga
    30301 agactcgaag gagtagaggc cccacatgat cgacatcaac aacttcgacg caatctcgat
    30361 cggcctcgct tcctcgaagc agatccgctc gtggtcaagc ggtgaggtca ccaagccgga
    30421 gacgatcaac taccgcacgc tcaagccgga gaaggacggc ctcttctgcg agcgcatctt
    30481 cggtcccacc aaggactggg agtgctactg cggcaagtac aagcgcgtcc gctacaaggg
    30541 catcgtctgc gagcgctgcg gcgtcgaggt cacgcgctcc aaggtgcgcc gcgagcgcat
    30601 gggccacatc gacctcgcgg cccccgtgag ccacatctgg ttcttcaagg gcgtcccgag
    30661 ccgcatcggc tacctcctcg acatggcgcc gaaggagctc gagaagatcc tctacttcgc
    30721 cgcgtccatc gtgacctggg tcgacgagga ggcgcgcgag aaggacacgg ccaagctgga
    30781 gaaggaggtc aacaaggtcc tcgacgccta cgcggccgag aaggaggagc gcctgctcga
    30841 gctgcgcgag tcgctcgagc gccgcgagga gtacctcaag accggctccc agaccggctt
    30901 catcgacgac gactacctgt gggcggaggc gctcgacgtc gacgtctcca agctcgacga
    30961 ggacgagcgc cagaagatgc tgcgcgacct gcgcaagacc ttcgactcgg acatcgccga
    31021 cacggaggcg tacatcgagg acgccgccga gcggat
//
LOCUS       DASVPL010000308         5072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_310626, whole genome shotgun sequence.
ACCESSION   DASVPL010000308 DASVPL010000000
VERSION     DASVPL010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310626"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3166
                     /locus_tag="VF517_17715"
     CDS             <1..3166
                     /locus_tag="VF517_17715"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX8054827.1"
                     /translation="SIGLAVDVERKDGSRSLVVPVVKGADALDLAGFVERYDELVAGA
                     RDNTLPPEAYSGANITLTNPGGLGTVASVPRLMPGQGTIIATGSIGLPPGLKGVDPSR
                     VRELGIAKVMTMTSTYDHRVIQGAESGAFLRRIDELLQGEDDFYTSAFAALGLGDAPI
                     APEAKADEAGAGPVTAHEPAPSEVKPDITGAADEALLQAVQAATSVVKAHRMHGHLAA
                     DLDPLRSEPMGDPALNPATVNLTPELMRQIPASVLRVAVPGETFADALPHLQDTYCGT
                     IAYEIEHISDHKQRVWLRQVIESGDHRWPPNAELRRRLLRRLSQVEALEQYLHKAFLG
                     RKQFSIEGLDMLVPMLDETIELASSAGAREVVIGMAHRGRLNVIAHTVGRGYRTILVE
                     FEGEQNLEADTALPSGGTGDVKYHYGANGTYKTEGGRNVKVTLSPNPSHLEFIDPVVE
                     GRARADQSSRGSRELSHDASLVLPVLIHGDAAFPAQGVVAETLNLQALEGYNTGGTVH
                     IITNNQLGFTTDPSEARSTRYASDLAKGFDVPIIHVNADDVEGCIAAVRLAMAFRDRF
                     GRDMLIDLIGYRRFGHNEQDEPAYTQPLIYERIKRHPPVRKLYADKLVAQGIVSQEEA
                     DGVAEQAYAELADAHQELKAALQQGSDTEEHELDRTASQEPRTTVAQDTLRALNEQLL
                     RVPEGFSVHRKLKPLFERRRSAVDEGTIDWAHAEQLAWASLLAQGVPIRLTGQDTERG
                     TFSQRHLVLHDAQTGDRWAPIQNLPKAEVPFELHNSPLSETACLGFEYGYSAQAPDAL
                     VLWEAQFGDFVNGAQVIVDQFMVSGLAKWGQATRLTLLLPHGYEGSGPEHSSGRLERF
                     LQLGAEGNIRVANCSTPAQYFHLLRRQALIQKRRPLVVMTPKSLLRNPAATSDLDELA
                     DGHFQPVLDDPTLAGPREKVTRLVLCAGKVYYDLVGHERRPELDHLAVCRVELLYPFA
                     ENELRELMASYPNLKTVVWAQEEPKNMGARHVMEGRLSWILPSGVGYEYIGRQLRASP
                     GEGYAAAHRTEQARIVREALGVRE"
     gene            complement(3184..3981)
                     /locus_tag="VF517_17720"
     CDS             complement(3184..3981)
                     /locus_tag="VF517_17720"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8054828.1"
                     /translation="MNLSGKTTLITGASGGLGNAIARALHSRGAIVKLTSRRAEVLEA
                     LAGELGDRAEVLPADLSSADDVRRLASEAGAVDILIANAGLPGTGRLGEYEPEQIDRV
                     LDVNLGSAIHLTHALLPRMLERRSGHLIYVSSMSGKVASPRASLYNATKFGLRGFSLA
                     MHDDLRGTGVGCTTIFPGFILDAGMFADSKLEAKPGSGPRRTEDVTDAVLRALAKNPY
                     EIDVAGFVPRSGGWLTGMAPALVSALQRAAGGEKAAEDLAGTQLDKR"
     gene            complement(3978..4898)
                     /locus_tag="VF517_17725"
     CDS             complement(3978..4898)
                     /locus_tag="VF517_17725"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX8054829.1"
                     /translation="MKAVCLGAHVLDVLARPVTEIPEGQGGALLEEIRMAPAGSAGGT
                     AVVLAKLGAEVVSAGAIGRDPLGDTLIKLLQEHGVDTGSLARKADVQTSASVLPIRPN
                     GDRPALHVIGANGALTEDDIPWQAIETATHVHLGAPEIAGPELCAKVAERARAGGAVT
                     SADMLAPGEPGILEWVAPALAHVDHLLVNEEQASGLAGEQDVAVACAKLRERGPEVIA
                     ATLGAEGAVVVSGDGEWRVPAHEVEVVDTSGCGDAFSAGYLRGLADGRPLAEAAALGC
                     AAATFVAGGLGSDAGEFDLAAAERLAFGGS"
BASE COUNT          735 a         1921 c         1772 g          644 t
ORIGIN      
        1 gagcatcggg ctcgccgtgg acgtggagcg caaggacggc agccgcagcc tcgtggtgcc
       61 ggtcgtcaag ggggccgacg ccctcgacct cgccggcttc gtcgagcgct acgacgagct
      121 cgtggcgggc gcccgcgaca acacgctccc gcccgaggcg tactccggcg cgaacatcac
      181 cctcacgaat ccgggggggc tcggcaccgt cgcctccgtg ccgcgcctga tgcccgggca
      241 gggcacgatc atcgccacgg gctcgatcgg cctcccgccc ggcctcaagg gggtcgaccc
      301 gagcagggtc cgggagctcg gtatcgcgaa ggtcatgacg atgacgagca cctacgacca
      361 ccgcgtgatc cagggcgcgg agtcgggggc gttcctgcgg cggatcgacg agctgctcca
      421 gggcgaggac gacttctaca cgtccgcctt cgcggcgctc ggcctcggcg acgccccgat
      481 cgcgcccgag gcgaaggccg acgaggcggg cgccggtccg gtcaccgccc acgagcccgc
      541 gccgtccgag gtcaagcccg acatcaccgg cgcggcggac gaggcgctgc tccaggcggt
      601 ccaggccgcc acctccgtgg tcaaggcgca ccgcatgcac ggccacctgg ccgcggacct
      661 cgatcccctc cgctccgagc cgatgggcga cccggcgctg aacccggcca ccgtgaacct
      721 caccccggag ctgatgcggc agatccccgc gtcggtcctg cgcgtggcgg tccccggcga
      781 gacgttcgcg gacgcgctcc cccacctcca ggacacctac tgcggcacga tcgcctacga
      841 gatcgagcac atctccgacc acaagcagcg cgtctggctg cggcaggtca tcgagtcagg
      901 cgaccaccgc tggccgccga atgccgagct gcgccgccgg ctgttgcggc gcctgtcgca
      961 ggtcgaggcg ctcgagcagt acctgcacaa ggcgttcctc ggccgcaagc agttctcgat
     1021 cgaaggcctc gacatgctcg tgccgatgct cgacgagacg atcgaactcg cgtccagcgc
     1081 gggcgcgcgc gaggtcgtca tcggcatggc ccaccgcggc cgcctgaacg tgatcgcgca
     1141 caccgtcggg cgcggctacc gcacgatcct cgtggagttc gagggcgagc agaacctcga
     1201 ggccgacacc gccctgccgt ccggcggcac cggcgacgtg aagtaccact acggcgcgaa
     1261 cgggacctac aagaccgagg gtggccgcaa cgtgaaggtc acgctgtcgc cgaacccgag
     1321 ccacctcgag ttcatcgacc cggtggtcga gggccgcgcg cgcgccgacc agtcgagccg
     1381 cggcagccgc gagctctcgc acgacgcctc gctcgtcctg cccgtgctga tccacggcga
     1441 cgccgccttc ccggcccagg gcgtggtggc cgagacgctc aacctccagg cgctcgaggg
     1501 ctacaacacg ggcggcacgg tccacatcat caccaacaac cagctcggct tcaccacgga
     1561 cccgagcgag gcgcgctcga cccgctacgc ctcggacctc gccaagggct tcgacgtccc
     1621 gatcatccac gtgaacgccg acgacgtgga gggctgcatc gccgccgtgc ggctcgcgat
     1681 ggcgttccgc gaccgcttcg gccgcgacat gctgatcgac ctgatcggct accgccgctt
     1741 cggccacaac gagcaggatg aacccgccta cacgcagccg ctgatctacg agcggatcaa
     1801 gcgccacccg ccggtccgca agctctacgc ggacaagctg gtggcgcagg ggatcgtctc
     1861 ccaggaggag gccgacgggg tggccgagca ggcgtacgcc gagctggccg acgcccacca
     1921 ggagctgaag gccgccctcc agcagggctc cgacaccgag gagcacgagc tcgaccgcac
     1981 ggcctcgcag gagccgcgca cgaccgtcgc gcaggacacg ctgcgtgcgc tcaacgagca
     2041 gctgctgcgc gtgcccgagg gcttctcggt gcaccgcaag ctcaagccgc tgttcgagcg
     2101 ccgccgcagc gccgtggacg agggcacgat cgactgggcg cacgccgagc agctcgcgtg
     2161 ggcctcgctg ctcgcgcagg gcgtgccgat ccgcctcacc ggtcaggaca ccgagcgcgg
     2221 cacgttcagc cagcgccacc tcgtgctcca cgacgcgcag accggcgacc gctgggcgcc
     2281 gatccagaac ctgcccaagg ccgaggtccc gttcgagctc cacaacagcc cgctgtcgga
     2341 gacggcctgc ctcgggttcg agtacggcta ctcggcgcag gcgcccgacg cgctggtgct
     2401 gtgggaggcg cagttcggcg acttcgtgaa cggcgcgcag gtgatcgtgg accagttcat
     2461 ggtctccggg ctcgccaagt ggggccaggc cacgcggctc acgctgctcc tcccccacgg
     2521 gtacgagggg tcggggccgg agcactcgag cgggcggctc gagcgcttcc tccagctcgg
     2581 tgccgagggc aacatccgcg tggcgaactg ctcgaccccc gcgcagtact tccacctcct
     2641 gcgccggcag gcgctgatcc agaagcgccg cccgctcgtg gtgatgacgc cgaagagcct
     2701 gctgcgcaac cccgcggcca cctccgacct cgacgagctg gccgacggcc acttccagcc
     2761 ggtgctcgac gacccgacgc tggcgggccc gcgcgagaag gtcacgcggc tcgtgctctg
     2821 cgccggcaag gtgtactacg acctcgtcgg ccacgagcgc cgtcccgagc tcgaccacct
     2881 ggcggtctgc cgcgtcgagc tgctctaccc gttcgcggag aacgagctgc gcgagctgat
     2941 ggcgagctac ccgaacctga agaccgtcgt ctgggcccag gaggagccga agaacatggg
     3001 cgcccgccac gtgatggagg gccggctctc gtggatcctc ccctcgggcg tcggctacga
     3061 gtacatcggc cgccagctcc gcgccagccc cggcgagggc tacgccgccg cgcaccgcac
     3121 cgagcaggcg cgcatcgtgc gcgaggcgct cggcgtccgc gagtagcgct ccgcggtcgc
     3181 gctctagcgc ttgtcgagct gggtgccggc gaggtcctcg gcggccttct cgccaccggc
     3241 cgcgcgctgg agcgcggaca cgagcgccgg cgccatgccg gtgagccagc cgcccgagcg
     3301 cgggacgaag cccgccacgt cgatctcgta cgggttcttc gcgagcgcgc gcaatacggc
     3361 gtcggtcacg tcctcggtgc ggcgcgggcc gctgcccggc ttggcctcga gcttggagtc
     3421 cgcgaacatg cccgcgtcca ggatgaaccc ggggaagatc gtggtgcagc cgacgccggt
     3481 cccgcgcagg tcgtcgtgca tcgcgagcga gaagccacgc aggccgaact tggtggcgtt
     3541 gtagagcgag gcgcgcgggc tcgcgacctt gccggacatc gacgagacgt agatcaggtg
     3601 accgctgcgg cgctcgagca tccgggggag gagcgcgtgg gtgaggtgga tcgcggagcc
     3661 gaggttcacg tcgagcacgc ggtcgatctg ctcgggctcg tactcgccga gacgccccgt
     3721 gccgggaagc cccgcgttgg cgatcaggat gtcgacggcg cctgcctcgg acgcgagccg
     3781 gcgcacgtcg tccgcgctcg acaggtcggc ggggagcacc tcggcgcggt cgcccagctc
     3841 gccggcaagc gcctcgagca cctcggcccg cctgctcgtg agcttcacga tcgcgccacg
     3901 ggagtgcagg gcacgtgcga tcgcgttgcc gaggccgccg ctggcgcccg tgatgagagt
     3961 ggtcttgccg ctgaggttca tgagcctccg aaggcgagcc gctcggcggc cgccaggtcg
     4021 aattcgccgg cgtcgctacc gaggccaccg gccacgaacg tcgccgccgc gcagccgagg
     4081 gcggccgcct cggcgagcgg gcgaccgtcg gccagcccgc gcaggtagcc ggcgctgaac
     4141 gcgtcgccgc agcccgaggt gtccacgacc tccacctcgt gggccggcac gcgccactcg
     4201 ccgtcgccgg agaccacgac cgccccctcc gcgcccaggg tggccgcgat cacctccggc
     4261 ccgcgctcgc gcagcttcgc gcacgccacc gccacgtcct gctcgccggc gaggcccgac
     4321 gcctgctcct cgttcaccag caggtggtcg acatgcgcca gtgcgggggc cacccactcc
     4381 aggatcccgg gctcgcccgg cgccagcatg tcggccgagg tgaccgcacc gcccgcacgg
     4441 gcgcgctcgg ccaccttcgc gcacagctcc ggcccggcga tctcgggcgc gccgaggtgg
     4501 acgtgcgtgg cggtctcgat tgcctgccac gggatgtcgt cctcggtgag cgcgccgttg
     4561 gcgccgatca cgtggagcgc gggccggtcg ccgttcgggc gaatcggaag gaccgaggcc
     4621 gaggtctgga cgtccgcctt gcgcgcgagt gagccggtgt cgacaccgtg ctcctggagc
     4681 agcttgatca gcgtgtcgcc cagcgggtcg cgcccgatcg ccccggcgct caccacctcg
     4741 gcgccgagct tcgccagcac cacggccgtt ccgccggccg agccggccgg cgccatccgg
     4801 atctcctcga gcagcgcgcc gccctgcccc tccgggatct cggtgaccgg gcgcgcgagc
     4861 acgtcgagca cgtgggcgcc gaggcagacg gccttcatcg cgccacctcg cgcgtggtgc
     4921 cgtagccgag cgtcgcgacc tgatcgacca cggcccgcag gtcgtcgtcg gtcaggctgc
     4981 gcggctcgcc gagctgcgcc gcgcgccagt agacctcgca ggcccactcc agcagcagcg
     5041 ccagcccgac cgccgactgc atgtcggccg cg
//
LOCUS       DASVPL010000309         7147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_233324, whole genome shotgun sequence.
ACCESSION   DASVPL010000309 DASVPL010000000
VERSION     DASVPL010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233324"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VF517_17730"
     CDS             <1..696
                     /locus_tag="VF517_17730"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8054830.1"
                     /translation="FRFFGEALRQAGVLILGSTMVIWSLAFILGLQCGIEGAYFNRSV
                     GAPAYAGVFSAWCDLREIMPYAFGYMMSAKVGTGLVAEIGAMRITDEIDALEVMGISS
                     MTFLCATRLLAAFMVLPFMYIAAVGAGFFASYLAVVEQIGEVSSGGFFLIFWMFQNPP
                     DLLYSLIKGMAMATVIVIVGTYYGYTASGGPVGVGTATAKSMVLNTVMVHLVGMLGTQ
                     IFWGANPRAPIGG"
     gene            719..1867
                     /locus_tag="VF517_17735"
     CDS             719..1867
                     /locus_tag="VF517_17735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8054831.1"
                     /translation="MIDERGNDDQATPPSGYDVPAGEQETVSWESPSAETREAQAPPP
                     PPTQEDRVPTPWELQQQQGGQETVAAEQPNPVGGAAVTNEPESSRGAWEAAETRRAGE
                     LEGEAAPPSGQDDDEYKWHTTRTGRSESAESDAVEFIDVKKSFGRNAVLNGLNLGIPE
                     DQISMILGPSGTGKSVCIKHMVGLLYPDEGDVLVHGESVPNMRDDDLFEMRKKFGVLF
                     QDGALFGSMNLFDNVAFPLRQHTDKGEEEIVEICTRRLREVGLAGAGEKMPNELSGGM
                     RKRAGFARALVLDPQIVLFDEPDSGLDPVRTALLCELIKEIHEENGGAYVVITHDIMS
                     ARRVAEYIAILWKGRIVESGPASELFNSDNQFVRQFLSGEARGPLGME"
     gene            1949..2233
                     /locus_tag="VF517_17740"
     CDS             1949..2233
                     /locus_tag="VF517_17740"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8054832.1"
                     /translation="MNDYKLAVVKAKGEFVWHKHDDTDDFFLVVDGHLTIQLRDRDVE
                     LGPGDLYVVPRGVEHCPRADEEAHVLLIEPRGTPNTGDAGGELTAEEREI"
     gene            complement(2208..3167)
                     /locus_tag="VF517_17745"
     CDS             complement(2208..3167)
                     /locus_tag="VF517_17745"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054833.1"
                     /translation="MRVGIAAVPRCWDSGLTTLLDVLRTANDVRALVDRSIEPVDVRL
                     VGSARHVTTGGGLTLATDLLVGDDGALAELDVFVVPGLGLATPASLAEGLASAPVRRL
                     RNWLARSEGGPALAAACSGTFVLADAGVLDGHAATTSWWLAGEFRRLYPRVELDMSRM
                     VVHSGAVTTAGAAFAHIDLGMSLVSRVSPKLADTVARFLLVDERPAISLEAATGHLAT
                     ADSLVTEFEDWVRERLDGDVAISAAAAEIGTTRRTLERHCRTRTGLTPHDLVKRLRVE
                     RANHLRRTTNLSYDQIAPMVGYRNGSTLRALLRRQARSPAPPR"
     gene            3228..3959
                     /locus_tag="VF517_17750"
     CDS             3228..3959
                     /locus_tag="VF517_17750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX8054834.1"
                     /translation="MKIEMLDGGAFTSPAGLWRRDEDMDRRMRFPIPAYLIETADERI
                     LVDTGLHPEAAADTARRYPGAEQLAIFELELARGVGEQVDLATITRVVLTHLHFDHAG
                     GLALLPPSVPIVVQRREWEAAHDADAVARNFYLPADYADVRDRVVLVDGDHDLFGDGS
                     VELLLTPGHTPGHQSVRVGERVVIGGDVTHFASGLDDHRFPMFADDFAAQAASADRLR
                     ALRDAGAAVRPGHHPGVLAPGPVAV"
     gene            4061..6982
                     /locus_tag="VF517_17755"
     CDS             4061..6982
                     /locus_tag="VF517_17755"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8054835.1"
                     /translation="MLKRLFGWRPDVAGGLGRVARTVTGHPIATVAVVVALALVGLAL
                     ALRLEPSASTDTLVGRDTATFKATEKFRDRFGDDAVIIMVRGDLQRTILTEDLQRLVT
                     LEQCLAGNAPAAARAELPPVCDKLARAKPAQVVYGPGTFINVAVTEIQKGFQTRQAQA
                     AAQGKAAGANAVKLAKRQGLPRAEQERLRKAAEELAINRFTQEALQLGLRYGLALANP
                     AIDNPEFVSQLVFDTSKGPGIPKPRFAFLFPSPNAAMVQFRLRPNLTDAERTNAIALV
                     REAVAEPQFKLQRGGKYVVTGIPVVVAGLTDAVQRSIFILLAAAVLLMAITLLVVFRT
                     RLRTRLLPLGTALAAAAMTYGCMKLAGFDLTMASIAALPVLIGLAVDYAIQLQARYDE
                     NRYEEDPDGSIAARAAAARGGPLITGAALATAAGFLVLLLSPVPMIHGFAVTVIAGIV
                     LALVCALTAGLATLSRWSEPKPGVPAPLPRARELGARTWDRIALSRPGRLAGRIGARA
                     AAAAARAARGALGFALDRPRNVLVAGLALAAIGWAADTQTEFVSDVRKLVPQDLPALR
                     DINELERETGVSGEIDVMISGNDLTEPHVISWMTNFQKRVLEQHDWQTGKTCRQEKDP
                     PQLCPALSLTDLFRQTPGTGTDARSLLSAVPPYFSQAVITQDRRTANLAFGIRLQPLD
                     QQEQVVEQIRKTLEGRGGPPPGVQADIVGLPVLAAESNGKLAADWRRALMLLAGLAAV
                     FLVLLAIRRRFAAAAVPLIPIALATGWSSLILFLIGIPLNPMSATLGALVIAISTEFS
                     VLLSARYHSEREEGADPEEALELTYASTGAAVLASGATAIAGFAALIASDVRMLQQFG
                     IVTVVDLTVSLLGVMVVLPAALVWAEERSTVRWSDLRPRRLLREARWAFGDATADMQR
                     PALPRGGIPRPGLPSARALRRGGGRGWRPRVPGLAGRRPRMPGLRLPRRPRPGRRRTR
                     A"
BASE COUNT          993 a         2587 c         2572 g          995 t
ORIGIN      
        1 ttccgcttct tcggcgaggc gctgcgccag gccggcgtcc tgatcctcgg ctcgacgatg
       61 gtgatctggt cgctcgcctt catcctcggc ctccaatgcg ggatcgaggg cgcctacttc
      121 aaccgctcgg tcggcgcccc cgcctacgcc ggggtcttca gcgcctggtg cgacctgcgc
      181 gagatcatgc cctacgcgtt cggctacatg atgagcgcca aggtcgggac cgggctcgtc
      241 gccgagatcg gcgcgatgcg gatcaccgac gagatcgacg ccctggaagt catgggcatc
      301 agctcgatga ccttcctctg cgcaacgcgc ctgctggccg ccttcatggt gctgccgttc
      361 atgtacatcg ccgccgtcgg agccggcttc ttcgcgagct acctggcggt ggtcgagcag
      421 atcggcgagg tctcatcagg cggcttcttc ctgatcttct ggatgttcca gaacccgccc
      481 gacctcttat acagcctgat aaagggcatg gcgatggcga ccgtcatcgt gatcgtcggc
      541 acctactacg gctacaccgc ctcgggcgga ccggtcggcg tcggcaccgc caccgccaag
      601 tcgatggtgc tgaacaccgt gatggtgcac ctcgtcggca tgctcggcac gcagatcttc
      661 tggggagcca acccgcgggc gccgataggc ggctgacctt ccaagggagg agtacgactt
      721 gatcgacgaa cgcggaaacg acgaccaggc aacgccgccg tccggctacg acgtcccggc
      781 gggcgagcag gagacggtct cctgggagtc ccccagcgcg gagacgcgcg aggcccaggc
      841 cccgccgccc ccgcccacgc aggaggatcg cgtgccaacg ccgtgggagc tccagcagca
      901 gcagggcggg caggagacgg tggccgccga gcagccgaac cccgtggggg gagcggccgt
      961 cacgaacgag cccgagtcga gccgaggcgc gtgggaggcc gcggagactc gccgcgccgg
     1021 tgagctcgag ggcgaggccg caccgccgtc cggtcaggac gacgacgagt acaagtggca
     1081 caccacccgc accggccgca gcgagagcgc cgagtccgac gccgtcgagt tcatcgacgt
     1141 gaagaagtcg ttcggccgca acgcggtcct caacgggctc aacctcggca tccccgagga
     1201 ccagatctcg atgatcctcg ggccctcggg caccggcaag tcggtctgta tcaagcacat
     1261 ggtcggactg ctctacccgg acgagggcga cgtgctggtg cacggcgaat cggtcccgaa
     1321 catgcgcgac gacgacctgt tcgagatgcg caagaagttc ggcgtgctgt tccaggacgg
     1381 cgcgctgttc ggctcgatga acctgttcga caacgtcgcg ttcccgctgc gccagcacac
     1441 ggacaagggc gaggaggaga tcgtcgagat ctgcacccgc cgcctccgtg aggtcggcct
     1501 ggccggcgcc ggcgagaaga tgccgaacga gctctcgggc gggatgcgca agcgcgccgg
     1561 gttcgcgcga gcgctggtgc tcgacccgca gatcgtgctc ttcgacgagc ccgactcggg
     1621 cctcgacccg gtccgcaccg ccctcctctg cgagctcatc aaggagatcc acgaggagaa
     1681 cggcggcgcc tacgtggtga tcacgcacga catcatgagc gcgcgccgcg tcgccgagta
     1741 catcgcgatc ctctggaagg gccgcatcgt ggagtcgggc cccgcgtccg agctgttcaa
     1801 ctcggacaac cagttcgtgc gccagttcct ctccggcgag gcccgcggcc cgctggggat
     1861 ggagtagggc ccgctggacg acaaggtcaa cctcgccgac aagctcgccg cgctctcgga
     1921 gcacttcagc ccgggtatcg tcggctacct gaacgactac aagctcgcgg tcgtaaaggc
     1981 caagggcgag ttcgtgtggc acaagcacga cgacaccgac gacttcttcc tggtcgtgga
     2041 cgggcacctg acgatccagc tccgcgaccg cgacgtggag ctcgggcccg gcgacctcta
     2101 cgtggtcccg cgcggggtcg agcactgccc gcgggccgac gaggaggcac acgtcctgct
     2161 gatcgagccg cgcggcacgc ccaacaccgg cgacgccggc ggcgagctca ccgcggagga
     2221 gcgggagatc tagcctgccg gcgcagcagc gcccgcaggg tcgagccgtt gcggtagccg
     2281 accatcggcg cgatctggtc gtagctcagg ttggtcgtgc gcctcaggtg gttggcccgc
     2341 tcgacccgca ggcgcttgac caggtcgtgc ggggtcagcc ccgtgcgggt gcggcagtga
     2401 cgctcgagcg tccgccgcgt ggtcccgatc tcggccgccg ccgcggagat ggcgacgtcg
     2461 ccgtcgaggc gctcgcgcac ccagtcctcg aactcggtca cgagcgagtc ggcggttgcc
     2521 aggtggccgg tggcggcctc gaggctgatc gccggccgct cgtcgacgag caggaagcgc
     2581 gcgaccgtgt cggccagctt cgggctcacg cgggacacga ggctcattcc caggtcgatg
     2641 tgggcgaagg ctgcaccggc ggtggtgacc gccccgctgt ggacgaccat gcgcgacatg
     2701 tccagctcca ccctcgggta gagccggcgg aactcgccgg ccagccacca ggaggtggtg
     2761 gcggcgtggc cgtccagcac cccggcgtcg gccagcacga acgtgccgct gcacgcggcc
     2821 gccagcgcgg gcccgccctc gctgcgcgcg agccagttgc gaaggcgccg gacgggggcc
     2881 gacgccagac cctcggccag cgaggccggc gtggccaggc cgaggccggg gaccacgaac
     2941 acgtcgagct cggccagcgc gccgtcgtcg cccaccagga ggtcggtggc gagggtcagc
     3001 ccgccgccgg tcgtgacgtg cctagcgcta cccacgaggc ggacgtcgac gggctcgatc
     3061 gaccggtcca ccagggcgcg aacgtcgttg gccgtgcgga ggacgtcgag cagcgtcgtc
     3121 aggccggagt cccagcacct gggcactgct gcgatcccga cgcgcatgtc gcgaaccgta
     3181 accgacgggt cgcatctcgt gccagggctg gacttagcct cgggagcatg aagatcgaga
     3241 tgctcgacgg cggcgcgttc acgtcacccg ccggactctg gcgccgggac gaggacatgg
     3301 accggcggat gcgctttccc attccggcct acctgatcga gaccgccgac gagcggatcc
     3361 tcgtcgacac cggcctccac cccgaggccg ccgccgacac ggcacgccgc taccccggcg
     3421 ccgagcagct cgccatattc gagctcgagc tcgccagggg cgtcggcgag caggtcgacc
     3481 tcgcgacgat caccagggtg gtgctcacgc acctccactt cgatcacgcg ggcggcctgg
     3541 cgctgctgcc gccctcggtt ccgatcgtcg tccagcgccg cgagtgggag gcggcccatg
     3601 acgcggacgc tgtggcgcgg aacttctacc tgcccgccga ctacgcggac gtgcgcgatc
     3661 gggtcgtgct cgtggacggc gaccacgacc tgttcgggga cgggtccgtc gagctgctgc
     3721 tcacgcccgg ccacacgccg gggcaccagt cggtgcgagt cggcgagcgg gtggtgatcg
     3781 gcggcgacgt cacgcatttc gcgtcgggcc tcgacgacca ccgattcccg atgttcgccg
     3841 acgacttcgc ggcgcaggcg gcgtcggccg atcggctgcg cgcgctgcgc gacgccggag
     3901 cggccgtccg gcccgggcac caccccggcg tgctcgcgcc cggcccggtg gcggtttgac
     3961 ccgcgcgggg cccggctgag cgcggacgcg tcgcgttaag cgccggttgc gctgcccgcg
     4021 tgcggagcgg gccgacgtgc cggttgccta agctgagcgt gtgctgaaga ggttgttcgg
     4081 gtggcggccc gatgtcgccg gtgggctcgg gcgggtcgcg cgtacggtga ccggccatcc
     4141 gatcgcgact gtggcggtcg tggttgcgct cgcgctcgtc gggctggcgc tcgcgctgcg
     4201 gctggagccg agcgcttcga ccgacacgct ggtcggtcgc gacaccgcga cgttcaaggc
     4261 caccgagaag ttccgcgacc gcttcggcga cgacgccgtg atcatcatgg tgcgcggcga
     4321 ccttcagcgc acgatcctca ccgaggacct ccagcggctc gtcacgctcg agcagtgcct
     4381 ggccggcaac gcgccggccg cggcccgggc cgagctgccg cccgtctgcg acaagctcgc
     4441 gagggccaag ccagcccagg tggtctacgg gcccgggacg ttcatcaacg tcgccgtcac
     4501 cgagatccag aagggcttcc agacgcgcca ggcgcaggcc gccgcgcagg gcaaggccgc
     4561 gggtgcgaac gcggtcaagc tcgccaagcg ccaggggctc ccgagggccg agcaggagcg
     4621 gctgcgcaag gcggccgagg agctcgcgat caacaggttc acgcaggagg cgctccagct
     4681 cggcctgcgc tacggcctcg cgctcgccaa tccggcgatc gacaaccccg agttcgtctc
     4741 gcagctcgtg ttcgacacga gcaagggccc cggcatcccg aagccgcgct tcgcgttcct
     4801 gttccctagc ccgaacgcgg cgatggtcca gttccgcctg cgccccaacc tgaccgacgc
     4861 ggagcgcacc aacgcgatcg ccctcgtgcg cgaggcggtg gcggagccgc agttcaagct
     4921 ccagcgcggc ggcaagtacg tcgtgaccgg gatcccggtc gtggtggcgg ggctcaccga
     4981 cgcggtccag cgctcgatct tcatcctgct cgcggccgcg gtgctgctga tggcgatcac
     5041 gctgctcgtc gtcttccgca cgcgcctgcg cacgcggctg ctcccgctcg gcaccgccct
     5101 ggccgcggcg gcgatgacct acggctgcat gaagctcgcc gggttcgacc tgacgatggc
     5161 gtcgatcgcc gcgctgccgg tgctgatcgg cctggccgtc gactacgcga tccagctcca
     5221 ggcgcgctac gacgagaacc gctacgagga ggacccggac ggcagcatcg ccgcgcgcgc
     5281 cgctgcggcc cgcggtggcc cgctcatcac cggcgcggcc ctcgccaccg ccgcgggctt
     5341 cctcgtgctg ctgctctcgc ccgtgccgat gatccacggc ttcgcggtca ccgtgatcgc
     5401 cgggatcgtg ctcgcgctcg tgtgcgcgct caccgcgggc ctcgccacgc tctcgcgctg
     5461 gtcggagccg aagccggggg tgccggcgcc gctcccgcgc gcgcgtgagc tgggcgcccg
     5521 gacctgggac cggatcgcgc tgagccgccc gggccgcctg gccggccgca tcggcgcccg
     5581 cgcggccgcg gccgccgccc gcgcagcgcg cggcgcgctg gggttcgcgc tcgaccgccc
     5641 ccgcaacgtg ctggtcgcgg gcctcgcact cgcggcgatc ggatgggccg cggacacgca
     5701 gaccgagttc gtgtcggacg tgcgcaagct cgtcccgcag gacctccccg cactcaggga
     5761 catcaacgag ctcgagcgcg agaccggcgt ctccggcgag atcgacgtga tgatcagcgg
     5821 caacgacctg accgagccgc acgtgatctc ctggatgacc aacttccaga agcgcgtgct
     5881 cgagcagcac gactggcaga cgggcaagac gtgccgccag gagaaggacc cgccacagct
     5941 ctgcccggcg ctctcgctca ccgacctgtt ccgccagacg cccggcacgg ggaccgacgc
     6001 gcgcagcctg ctctcggccg tgccgcccta cttctcgcag gccgtgatca cccaggaccg
     6061 ccgcaccgcg aacctcgcgt tcggcatccg gctccagccg ctcgaccagc aggagcaggt
     6121 ggtcgagcag atccgcaaga cgctcgaggg gcgcggcggc ccgccgccgg gggtccaggc
     6181 ggacatcgtc ggcctgcccg tgctcgcggc cgagtcgaac ggcaagctcg ccgccgactg
     6241 gcgccgcgcg ctcatgctgc tggcgggcct ggccgcggtc ttcctcgtgc tgctggcgat
     6301 ccgtcggcgg ttcgccgccg cggccgtacc gctgatcccg atcgcgctcg cgaccggctg
     6361 gtcgtcgctg atcctgttcc tgatcggcat accgctgaac ccgatgtcgg ccacgctcgg
     6421 cgcgctggtg atcgcgatct cgaccgagtt cagcgtgctc ctctcggcgc gctaccacag
     6481 cgagcgcgag gagggggccg acccggagga ggcgctcgag ctgacgtacg cctcgaccgg
     6541 cgcggcggtg ctggcctcgg gcgcgaccgc gatcgcgggt ttcgcggccc tgatcgcgtc
     6601 ggacgtgcgg atgcttcagc agttcggcat cgtcacggtg gtggacctga cggtctccct
     6661 gctcggcgtg atggtggtgc tgccggccgc gctcgtgtgg gcggaggagc gcagcacggt
     6721 tcgttggagc gatctgcggc cgcggcggct gctgcgcgag gcgcgctggg cgttcggcga
     6781 cgcgaccgcc gacatgcagc gcccggcgct cccgcgcggg gggatcccgc gcccggggct
     6841 gccgtcggcg cgtgcgctgc gccgcggcgg cgggcggggc tggcgaccgc gcgtgccggg
     6901 gctcgccggg cggcgcccgc ggatgccggg gctccgcctc ccgcgccgcc cgcgcccggg
     6961 ccggcgccgc acccgtgcct gaggaccgct tcgcggacct cggtccggag cgcgggaaga
     7021 agtccgccgc cgagcgcttc gccgagctcg atgagcgcga gccggagcag gagccccggg
     7081 ggccgcgcga gccgcagcgc ccgggcggtc gctacggctg ggtggtcggc atcgcgttcg
     7141 tgatcgt
//
LOCUS       DASVPL010000310        22598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_115708, whole genome shotgun sequence.
ACCESSION   DASVPL010000310 DASVPL010000000
VERSION     DASVPL010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22598
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115708"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF517_17760"
     CDS             <1..342
                     /locus_tag="VF517_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8054836.1"
                     /translation="AIDAFAAHVCGPQALDLLGRARTEASHAAARAFAADFGASARWS
                     LPPRALRAIEAPVTIVVGERSERVWHDAARALEETLPRAELVVAAASGHLVPIEAPDV
                     LADAIRRLAQP"
     gene            complement(333..839)
                     /gene="def"
                     /locus_tag="VF517_17765"
     CDS             complement(333..839)
                     /gene="def"
                     /locus_tag="VF517_17765"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX8054837.1"
                     /translation="MAVRGVLHVPHPTLKQTARPLGEGERAEAERVAGDLLDTMRSFP
                     RCVGIAAPQIAELVRLVVVDVSEHPKATVRSGELVLVNPRVVESSGATVAREGCLSIP
                     ELTANVRRATHISVEAVRPDGSPVALETEGFEARCVLHELDHLDGVLFLDRVDSLATD
                     VFRRKSYG"
     gene            complement(846..1391)
                     /locus_tag="VF517_17770"
     CDS             complement(846..1391)
                     /locus_tag="VF517_17770"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX8054838.1"
                     /translation="MRRLALTAIGHDRPGIVAAVSRVLLAHRANVEDSQMTILRGHFT
                     MMLVVALADDADVNTLRDDLDDVREELALEAILLSPLEEVDPGGEPVPTHMVTLYGAD
                     HPGIVHAATSVLAERGVDITDLTTKLVNEAGGAPPLYALMMEIAPPSEAPEDELEDAL
                     RRVAEDQGVEVSLRRLESEAL"
     gene            1433..1636
                     /locus_tag="VF517_17775"
     CDS             1433..1636
                     /locus_tag="VF517_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054839.1"
                     /translation="MALTPEQRRRRDQVETLIRLMAPGLNLMLAAGERLSRMVAPDDD
                     EYYPIRTGTADPELPPRTGAADR"
     gene            1765..2559
                     /locus_tag="VF517_17780"
     CDS             1765..2559
                     /locus_tag="VF517_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054840.1"
                     /translation="MVDSYKEIDSNRGMLIVAFVFQSLSALVVALVLRYLHRASAYRA
                     PDDEPRIAWRLATIAPIVLAIAAILLAFMQLGIVDKVIDALPLSEQGVENIETDQRST
                     TTSLIVQGIATAAALSLAVAFILISRYARRAGLLSPFMGILGIIVGVILVLGPLLGQV
                     LGALPVVQWFWLGSLAMIFVGRWPGGRGPAWESGEAEPWPSAAELRAQAQADAGDDEL
                     ARPMRRREAAEPERGAEPQLDGAELDAEPAAPAHPRSKKRKRKRRR"
     gene            2724..3479
                     /locus_tag="VF517_17785"
     CDS             2724..3479
                     /locus_tag="VF517_17785"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8054841.1"
                     /translation="MYGRGAAYELNPHLAPDSGSDYACPYRECDGTGFVIDDETDSAV
                     PCTCRAGRIASARARNLAHEIPKRFRDLALDRNPAREILAGLGAQAMEYREFCERIGE
                     RLDQGKGLWFKGDRGTGKTTLAMLISIHAMRARRTVGIYTAPKLLESIRATYADDSPV
                     TLTQLTDRLRTVDLLHIEDLAVPRTNDWVLEQLYTIVNDRYQDQRSIVFTADIESLGD
                     LGERIGHRTASRLIDICGEPILMHGEDYRLRPT"
     gene            complement(3512..5485)
                     /gene="acs"
                     /locus_tag="VF517_17790"
     CDS             complement(3512..5485)
                     /gene="acs"
                     /locus_tag="VF517_17790"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX8054842.1"
                     /translation="MSDSPEQREDLEVLLRDEDRFAPPEEFAKRAKFSDPAVYDEAEE
                     DFEAWWERWAKELDWFEPWETVLDWDPPWAKWFKEGKLNASHNCLDRHVAAGNGDKVA
                     YHWVGEDGLTLDLTYAQLLADTQRFANVLKGIGVGPGDVVGIYMPMVPQTPAAMLACA
                     RIGAVHNVVFGGFSVESVKERMEVSDAKVLVTADETLRRGKPNPMKAKVDEILGDLPE
                     LQHVVVVKRTGGECPMTEGRDIWWHEALEEAEPECPAEPLDAEHPLYILYTSGSTAKP
                     KGIVHTTGGYLTGVAATHKVVFDLEPDTDVYWCAADVGWVTGHSYIVYGPLANGCTSV
                     LYEGAPDYPDKDRWWEIVEHYGVTILYTAPTAIRACIKWGDEYPEKHDLTSLRLLGSV
                     GEPINPRAWAWYHKVIGGERCPVVDTWWQTETGHIMITPLPGITETKPGSATKVFPGI
                     EAAVVNRDGEIVEEGGGFLTLRRPWPGMLRTLYKEDERYVETYWDKWGRDVYVVGDAA
                     KIDEDGYFWIVGRTDDVINVSGHRMSTMEIESAIVSHDKVAEAACVGAKDEDTGQAIV
                     AFVTIKGSEEEEEGFDEVIREHVAQKIGKLARPKRIVYADDLPKTRSGKIMRRLLRDI
                     AEGRELGDVTTLRDPSVTEIIRDKVASGQADEG"
     gene            complement(5493..6179)
                     /gene="rpe"
                     /locus_tag="VF517_17795"
     CDS             complement(5493..6179)
                     /gene="rpe"
                     /locus_tag="VF517_17795"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX8054843.1"
                     /translation="MSVATDLLRDRRVAPSILAADYTRLGGQVREVMDAGARVIHVDV
                     MDGHFVPPITIGPLIVSALADQVHGAGGILDVHLMVERPERHVDEFVRAGADSISVHF
                     EATPHVHYAVKAIHEAGCLAGVAITPSTPIDAFDEVASDIEIALCMTVNPGWGGQPFI
                     EATTGKIERMRTRLDPGIKIQVDGGIDATTGPRAAVSGARLFVAGSSVFSDPEPGVAY
                     TKLARAVDAV"
     gene            complement(6303..7145)
                     /locus_tag="VF517_17800"
     CDS             complement(6303..7145)
                     /locus_tag="VF517_17800"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8054844.1"
                     /translation="MRRTRILTTLVVVGTLGLTLVAVAFAATITGTDRGEKLRGTNAG
                     DTIDGNGGRDKIYAFAGDDIVSGGSGGDFIRGGSGVDNVSGDSGWDLILGGDGNDAVL
                     NGGDGRDRVHGGDGDDSLDGGAHRDLLWGGDGTDTTNGGDGNDRIAGGRGNDTSNGGA
                     GNDLIFANLGVDTTDGGPGNDDLWALARGDVNRDVQLDTTGDTLVGGEGDDRFHLRDG
                     EQDTVNCGPGTDLALLDTADVIADATPENANGSCEDVRRAAPKSKDQKQGKQQDNDKG
                     SKRD"
     gene            7281..8189
                     /locus_tag="VF517_17805"
     CDS             7281..8189
                     /locus_tag="VF517_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054845.1"
                     /translation="MTRKVTLAAFALALVLALVPAGGAAAAPQGEQISSASAFPPLTE
                     ASARRLALKLARSVAHKRNVRSWQLSEALSVRPNRVVFGYADRNRKEVFCTARLVVER
                     SATRRRAFISAPECAGIPGEALEIERATSAVVRAVAGQRPDARLSVRRYEKDLRDCEG
                     LVVPRGRHAEVELLYDAGATLASVDPLLTHLDAFVTRLQDIQPEDTELASGVVWWRRL
                     ITSLGSLPRTTLRPCTAILEWANDNYSTDTAPVDFAELQRTLDTLKRENRGILRAAEH
                     LAELGVAPRLSAGFTPDVLIATALGR"
     gene            8240..9379
                     /locus_tag="VF517_17810"
     CDS             8240..9379
                     /locus_tag="VF517_17810"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8054846.1"
                     /translation="MDFDSLYDELRDPLTRRIERVVGDPRTAEDLRQEAFARAWTSAP
                     RDAGHGQMRAWLHRTAHNLAVDELRRRRVRDWVPYDDELAPAFEDGDADERIAAREAL
                     AALTPHDRFLLLMRFEGGLSHAEIGQLLGIGADAARKRVARARTALAAAHRKVTPRER
                     PLVLVLVGDEDLAPYESWINAAGGEARPLDTTRFERQLASADALVITGSETDIHPGLY
                     GEPVRGSVGDLDLARDRRDLAAVRAALLQDVPLVGVCRGHQLLNIALGGTLHQDIART
                     PHTDLGGRRGRALCHRGEEHPIRTGSRTLARRVLGRGATVASCHHQAAKRVGDGLRAT
                     SVSPDGVVETLELPQRRFAIGVQWHPEVGGSASESLASALVEAAR"
     gene            9376..9678
                     /locus_tag="VF517_17815"
     CDS             9376..9678
                     /locus_tag="VF517_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054847.1"
                     /translation="MSGALGIRWACMHDAPALARLAALDSAAPLRGEVLVAEVDGVLR
                     AALAPAEGRAIADPFEPTAELVALLRLRAEQAARAEPRPGRLRRLRSPPAPQTAST"
     gene            complement(9790..11379)
                     /locus_tag="VF517_17820"
     CDS             complement(9790..11379)
                     /locus_tag="VF517_17820"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX8054848.1"
                     /translation="MDYPVERFTEEERDRLAPHFTNLDRPVFGLVNLPETVKGALFAR
                     YSRYPGTLRRLYLEEFADDVASGGGGYDGAEGERAAKLYERVFVGYGDDSVAQLGGAH
                     IACEWVSNVLTKVLQRGRLAAYLEQSTRYIPYDGAMPGGGYRYWRDDSLGPEYARAMD
                     ALFDAYAEALPRVEAWADERFPRSEGEPEAAHRRSIRAKALDLLRGLLPAASLSHMGI
                     FATGQAYEQLVMRLLASPLPEAREVGAMVLTELKAMMPSFVSRVDRPERGGEWIRVIG
                     ERREAAGRLAHRMGLDRPREEDAEGPSVRLLDVQGGEDDLLAAMLFEQAAVSEEELRS
                     AVSVLPPDERAALIAELVGDRANRRHRPGRGFEAIRYRFEVVSDYGAFRDLQRHRLLT
                     IQWQPLTPYLGAGVPAELEEAGVADLYRAGLDRSREEYERLVDAGMADLAPYALCLGF
                     RIRYVMDMNAREAMHTIELRSGREGHPGYRAVAHEMHAQIARVHPAVAAAMTHVDHDV
                     EPRLERILSEIRTHARRAEPV"
     gene            complement(11451..11648)
                     /locus_tag="VF517_17825"
     CDS             complement(11451..11648)
                     /locus_tag="VF517_17825"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX8054849.1"
                     /translation="MYVKPECPYCEAARGQMRQHGIEWEERDATTNAEWKSELFMYSP
                     RGVVPTIVEGGAVTVGHDGKG"
     gene            11687..13018
                     /locus_tag="VF517_17830"
     CDS             11687..13018
                     /locus_tag="VF517_17830"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX8054850.1"
                     /translation="MAVETELREVGRALAAAFPSNARRPLRALDERAMELTTRDQELR
                     AALFRLVDVTPACNSLDELARHLSGYLEDVDERPQPLRAAMRMADSAPGRRALGAAAA
                     AGVRHMAHRFIVAESPKAARRELRTLWGEGIGASLDLLGEATVTAEEADRYAARCADA
                     LDVLAADTARWPERPLLEHDSAGPIPRANLSVKVSALTPLMRPESPERGRDDAARRLR
                     PLLDRARDLGAHLHIDMESVDALETTLELVVDLLGEDAFHDGPSAGVVLQAYLRDSAA
                     ELDRILDWAERSPRATPLVVRLVKGAYWDHEVVEARQHGWTPPVFEDKAECDRNFEAL
                     TRRLLEARPLVRVAIASHNLRSVANAIATNRELGGDDADLELQVLRGLGDDLADALAA
                     SGFRVRSYCPVGELVAGMAYLVRRLLENTANESFLHVQASGASLDELLAPP"
     gene            13120..14631
                     /locus_tag="VF517_17835"
     CDS             13120..14631
                     /locus_tag="VF517_17835"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8054851.1"
                     /translation="MPVLVGDGARGPGEAAGGAGASGGGSASGGDPFESTDPGEPDRV
                     VAVAARATEADADAAVREAARAFPAWRDLGAPARAEVLVKAAAILRERRLELSALQVR
                     ECAKPWAEADADVCEAIDFLEYYAREAVRLAEGPELLQAPGERNTMRWEPRGVVAVIS
                     PWNFPLAIPMGMVAAGLATGNAVVLKPAEQSPGSALMLVEALRAAGVPPGALALLPGF
                     GDVGAALVRHPGVHTIAFTGSGKVGLEIVRAAAETPDGQGHVKRVIAEMGGKNSVIVD
                     SDADLDEAVPALVRSAFVYAGQKCSAAARVLAHERIAATLVERLAGATSVLEVGQAEG
                     FGIDVPPVIEREARDRVEGYAERAAGEGRIAARADAPRGAGWFVAPAVAAELPPDSPV
                     LTEEIFGPLLAVEPVPSVSAACDLLDASPFALTGGLFSRDPRAIEEVSRRTPVGNLYV
                     NRHITGAMVGRQPFGGNRRSGVGFKAGGPGYLLQFVEPRVITENTMRHGLVVE"
     gene            14618..15439
                     /locus_tag="VF517_17840"
     CDS             14618..15439
                     /locus_tag="VF517_17840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8054852.1"
                     /translation="MSSSRRLAEAARFRARLAAGTALDLLDVIAGRGDPLAPPRRLLG
                     DDYSEFERIGNEFLGHFVKLGGLEPHHRVLDIGCGLGRMAVPLTRYLTTAGSYEGFDV
                     ARLEIDWCRRTIGSRHPNFQFRVLDVRNERYNPGGSAQPTAWPFAGATFDFAFAASVF
                     THLRPDDASAYIAEAARVLRPGGTLFATFFLLDEETRPRIERGDSHFSFRAATDGPAA
                     AQDAGSFEAAVAYDLGWVRDRLGSAGLDVTDLRHGYWSAADDHLTWQDVIVARRR"
     gene            15489..17129
                     /locus_tag="VF517_17845"
     CDS             15489..17129
                     /locus_tag="VF517_17845"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8054853.1"
                     /translation="MPQLVLGPLLRYVGETEATLWVETSEPCEVAILSSRTPTFCVRG
                     RHYAIAYVKGLEPGCRYEYTVELDGEAVWPDPGSAMPAPSIRTPKEGERHKLIFGSCR
                     VGYPHEPPYNLTKDESPLGREVDALRAYALRMRDLPESEWPHLLLMIGDQVYADEVSP
                     HVKSFIRNRRDTTEGAGEQVADFEEYVRLYHESWGEPVIRWLLSTVSTAMIFDDHDVH
                     DDWNSSQAWLDEYRAKSWWKERIAGAFMSYWLYQHLGNLSPRDHDADELLQRARRVPD
                     LGDHLHEFALRADRDPAAHRWTFCRDVAKARVVVVDSRAGRVLDGTRKMLDDDEWRYL
                     EEHARGGFNHLFVASSLPFLLPRAVHDLEAWNEAVCDGAWGRPMIDVGERIRQGIDLE
                     HWAAFGDSFERVEGLLRAVASGERGDPPGTITMLSGDVHYAYLAEVGFPRGTGAKSPV
                     YQATCSPFRNPLDSKERTAVRFSCGRTAEAMARALARSAGARKPEIGWELREGPVFDN
                     QVATIEWEGPHAELILERAIPDGSVDPRLEETFRRTLS"
     gene            complement(17155..18972)
                     /locus_tag="VF517_17850"
     CDS             complement(17155..18972)
                     /locus_tag="VF517_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054854.1"
                     /translation="MNLFLVGWSGDGGVEPGAAAEALRRLLARMPFFPGRPVETWAAP
                     SGSLGAAWVTHGPEETGGIEYAATEPARLALFSGRPIRWTGEREADGRAPLDPAFYLG
                     QPDAWAPALDGRFAALRYDDADRTLAVVSDALGAYPLYEAHAGGVRWTSNNAELLRGL
                     IDGRELDPAVLATVLGGGWSLSGDPVWSGVRRLTYGCVRRLEPGRPAASAPLLPLGQI
                     APLIGAGFDAERAAGVLTAELAALADWPQRPNVVPVTGGRDSRVVLGAALHAGVDFES
                     TTGGDESSPDVQVGRRLAEVGGIGHSILAHDPHGSVWDDWRRAAAIVDMTSSGTASLA
                     DAAGFPLGPREGPLVLWHSGQGGEIARAYYGLGDGAAPDALTERLTRTFLGRRPGRTD
                     VLGDAGRELVRDQIAAWVERQLDAGVAALDVPDMFYLQRRMGTWAGPSHGCVEFVRDT
                     TSPLWSRRLLPDLLGLPATERARELFHLRVLEELAPELVEVPFEDGRGWPARRSGAAH
                     RMARARALAGKAAAEARRRALARRSAIAAAPDDPFARILLEIRDTVLSQPDHQAWAVL
                     DRPRVERLLGSPAAALDTMSRYYAWRLATVFGAMEPARR"
     gene            19029..19784
                     /locus_tag="VF517_17855"
     CDS             19029..19784
                     /locus_tag="VF517_17855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8054855.1"
                     /translation="MLNRLLPSLAAFSRGFALASPGGDVHEADGVMAAIVPELADRSV
                     VNCVVYDHAASLGARLGAIAAAYDEAGVRAWTVWTPESDHAAIRTLEAAGNVLDAEPT
                     AMGRELDGIEAPGAGELDLLPEPAPGDVADVLAPVYGWDTAARALVRWHPGYHPYLAL
                     ADGRPAVTLAVHDHDGDAHVTLVGTLEAARGRGLATLLLRRALADARDRGCTTTTLVA
                     TKMGRPVYARLGYRDLGRIQMWERRKPDTADAA"
     gene            19781..21202
                     /locus_tag="VF517_17860"
     CDS             19781..21202
                     /locus_tag="VF517_17860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8054856.1"
                     /translation="MSALRARPPVALAILLLLGVAARVWLMVDYRPAYLSSNDSARFL
                     HFAHIDDGMFEDTFGPTGYAAFLKVVRVVSDQIEFTIALQHLLGVLAAVLLYAALRRL
                     GAPVWAACVPAGATLLSGDQLYMEHTLLSEGPFLPLLAGGLYAGVRALEGERSMRWLA
                     LAGVLFAGTALFRNVGLILPPVLVAWAFVAMPGGVRGRLRSAAVAAGAAAAVLLVYAA
                     IAGLDGGNTGWTATSGWNAYGRSAPFADCDRFDPPAGTEELCEDTPPDRRPGSLFYLW
                     FDGSPARAKFKHPPLGQETLGKFGRRAILAQPLDYVKTVAADLPRFLNPELNKKRYSG
                     GGYFLMSRRGDVAERNVLYQLSRDYDHGRFSPGSSGDTIAEWQRLQRLGGVVPGLFVA
                     FALLGLAAARGVALRGLALFGLAGAGLVVMPAATLTLLGRYTVPPMPIVAGAGAIGAW
                     AIAARLRERRHARRGPAGENPSP"
     gene            21199..21591
                     /locus_tag="VF517_17865"
     CDS             21199..21591
                     /locus_tag="VF517_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054857.1"
                     /translation="MTTLRILVGALLVSTAIHYTDNWLSIEHYAPREGFVAENPGLVP
                     VAWVLFTVLGIAAYREYRRAPSLRAHLMLAGYSLAGITTFAHLLYEGNDFAAWQWASV
                     LADGITGSAVLGFALWSAARVRPAATVA"
     gene            21605..>22598
                     /locus_tag="VF517_17870"
     CDS             21605..>22598
                     /locus_tag="VF517_17870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8054858.1"
                     /translation="MSVELALKPPLFNPRTYDGAGLDEESRRLMLATIEFFESRGKAV
                     LKEHDRDLTWYADFLEFVEREGLFAKLLTPAAEGGGDPDKRWDTARICAFNEITGFYG
                     LAYWYTWQVSILGLGPIWQSENADAKRRAAELLDQGAIFAFGLSEKEHGADIYSTDMV
                     LTPDGEGGFRASGGKYYIGNGNLAGMVSVFGRRDDVEGADGYVFFAADSRHPSYELVK
                     NVVNSQSYVSQFRLNDYPVRAGDVLHTGKDAFYAALNTVNVGKFNLGFASIGICEHAY
                     YEAITHADARILYGNRVTDFPHVRRAFVDAYARLVAMKLFGDRSIDYMRSATPED"
BASE COUNT         2878 a         8408 c         8167 g         3145 t
ORIGIN      
        1 gcgatcgacg cgttcgccgc gcacgtgtgc gggccgcagg ctctcgacct cctcggccgc
       61 gcgcgcaccg aggcctccca cgccgccgcc cgggcgttcg cggcggactt cggggcctcg
      121 gcgcgatggt cgctgccccc gcgcgcgctg cgcgcgatcg aggcgccggt cacgatcgtg
      181 gtcggggagc gcagcgagcg cgtgtggcac gacgcggccc gcgcgctcga ggagacgctg
      241 ccgcgggcgg agctggtcgt ggccgccgcc tcggggcacc tcgtgccgat cgaggcaccc
      301 gatgtcctgg cggacgcgat ccgccgtctc gctcagccgt agctcttgcg ccggaacacg
      361 tcggtcgcga gcgagtcgac gcggtcgagg aacagcacgc cgtcgaggtg gtcgagctcg
      421 tgcagcacgc agcgcgcctc gaacccctcg gtctcgagcg cgaccggcga gccgtccggc
      481 cgcacggcct cgacggagat gtgcgttgcg cggcgcacgt tcgcggtcag ctcggggatg
      541 ctgaggcagc cctcgcgcgc gaccgtcgcg cccgaggact cgaccacccg cgggttcacg
      601 agcaccagct cgccgctcct cacggtcgcc ttcgggtgct ccgacacgtc cacgaccacc
      661 agccggacca gctcggcgat ctgcggtgcc gcgatgccca cgcagcgcgg aaacgagcgc
      721 atggtgtcga gcaggtcgcc cgccacgcgc tcggcctcgg cccgctcgcc ctcgccgagc
      781 ggccgggcgg tctgcttgag cgtcgggtgc ggtacgtgga ggacgccgcg gacggccatg
      841 gccgtctaga gcgcctcgga ctccagccgg cgcagcgata cctccacgcc ctggtcctcg
      901 gccacccgcc gcagcgcgtc ctccagctcg tcctccggcg cctccgacgg cggggcgatc
      961 tccatcatca gcgcgtagag cggcggcgct ccgccggcct cgttcacgag cttggtggtg
     1021 aggtccgtga tgtcgacgcc gcgctcggcg aggaccgacg tggccgcgtg gacgatgccc
     1081 gggtgatccg cgccgtagag ggtgaccatg tgcgtgggca ccggctcgcc gccggggtcc
     1141 acctcctcga gcgggctcag caggatcgcc tcgagcgcca gctcctcgcg tacgtcgtcc
     1201 aggtcgtcgc gcagcgtatt cacgtcggcg tcatcggcca gcgccaccac gagcatcatc
     1261 gtgaagtgac cgcgcaggat cgtcatctgc gagtcctcca cgttcgcgcg gtgcgcgagc
     1321 agcacgcggc tcacggcggc gactatcccg ggacggtcgt ggccgatcgc ggtcagcgcg
     1381 aggcgtcgca tcgcgccgga ccttaccggc gcccttccct agactcggcg ccgtggcact
     1441 gacccccgag cagcggcgcc gccgcgacca ggtcgagacg ctcatccgcc tgatggcgcc
     1501 ggggctgaac ctcatgctcg ccgcgggcga gcggctgtcg cgcatggtcg ccccggacga
     1561 cgacgagtac tacccgatcc ggaccggcac ggccgatccc gagctgccgc ccaggaccgg
     1621 ggcggcggac cgctgagcca caccgagcaa ctcgagtggg aagcgcgggc cgggcgcgtc
     1681 gcgtcggcgg ccgcgctcgc gtcgatcgcg ttcggcctcc tcgggctgtt cctcgccact
     1741 gcggcggctg gcagccacga gcacgtggtc gacagctaca aggagatcga ctcgaaccgc
     1801 gggatgctca tcgtcgcgtt cgtgttccag tcgctgagcg cgctcgtcgt cgccctggtg
     1861 ctgcgctacc tccaccgcgc ctcggcgtac cgagcccccg acgacgagcc gcggatcgcg
     1921 tggcgtctcg ccacgatcgc cccgattgtg ctcgccatcg cggcgatcct gctggccttc
     1981 atgcagctcg ggatcgtgga caaggtgatc gacgcgctgc cgctgagcga gcagggcgtg
     2041 gagaacatcg agaccgacca gcgctcgacc acgacgtcgc tgatcgtcca ggggatcgcg
     2101 accgccgcgg ccctgtcgct ggccgtggcg ttcatcctga tctcgcgcta cgcgcggcgg
     2161 gccgggctgc tctcgccctt catgggcatc ctcgggatca tcgtgggcgt gatcctcgtg
     2221 ctcgggccgc tgctcgggca ggttctgggc gcgctgccgg tcgtccagtg gttctggctc
     2281 ggatcgctcg cgatgatctt cgtcggccgc tggccgggcg ggcgcgggcc ggcgtgggag
     2341 agtggggagg ccgaaccctg gccctcggcg gcggagctgc gcgcgcaggc gcaggcggac
     2401 gcgggcgacg acgagctggc gcggccgatg cgcaggcgcg aggcggccga gccggagcgc
     2461 ggcgccgagc cgcagctcga cggggccgag ctcgacgccg agccggcggc cccggcgcac
     2521 ccgcgctcga agaagcgcaa gcgcaagcgc cgccgctgac gcgccggctc ccccgcgcac
     2581 gcgccgcgcg agccgccgcg ccggctccgc cgctgacgcc gcgccgccgc cgccgcgggt
     2641 cgctcgcgaa aactccgctg ccagcgggaa ccctcgcgcc agggccctgg caggcgtctg
     2701 atcgcgctgc ttgactggcg ggcgtgtacg gacgcggagc cgcctacgag ctgaaccccc
     2761 acctcgcgcc ggattcgggc agcgactacg cctgcccgta ccgcgagtgt gacggcaccg
     2821 ggttcgtgat cgacgacgag acggactcgg ccgtcccgtg cacctgccgc gcgggccgga
     2881 tcgccagcgc ccgcgcccgc aacctcgccc acgagatccc caagcgcttc cgcgacctgg
     2941 cgctcgaccg caacccggct cgcgagatcc tcgccggcct cggcgcgcag gccatggagt
     3001 accgagagtt ctgcgagcgc atcggcgagc ggctcgacca gggaaagggg ctgtggttca
     3061 agggcgaccg cggcaccggc aagaccacgc tggcgatgct gatctcgatt cacgcgatgc
     3121 gcgcgcgccg gaccgtcggc atctacacgg cgccgaagct gctcgagtcg atccgcgcca
     3181 cctacgccga cgactcgccc gtcacgctca cccagctcac cgaccggctc cgcacggtgg
     3241 acctgctcca catcgaggac ctcgccgtac cgcgcaccaa cgactgggtg ctcgagcagc
     3301 tctacacgat cgtcaacgac cgctaccagg accagcgctc gatcgtcttc accgccgaca
     3361 tcgagagcct gggcgacctg ggcgagcgga tcgggcaccg caccgcctcc cggctcatcg
     3421 acatctgcgg agagccgatc ctcatgcacg gcgaggacta ccggctcagg ccgacctagc
     3481 agccccccgg gccgggcgca gcagccgtcg cctaaccctc gtcggcctgc ccgctcgcca
     3541 ccttgtcgcg gatgatctcg gtgacgctcg ggtcgcgcag ggtcgtcacg tcgcccagct
     3601 cgcggccctc ggcgatgtcg cgcagcagcc ggcgcatgat cttgccggag cgcgtcttcg
     3661 ggaggtcgtc ggcgtagacg atccgcttcg gccgcgcgag cttgccgatc ttctgcgcca
     3721 cgtgctcgcg gatgacctcg tcgaagccct cctcctcttc ctcggacccc ttgatcgtca
     3781 cgaacgcgac gatggcctgg ccggtgtcct cgtccttcgc acccacacaa gcggcctccg
     3841 ccaccttgtc gtgggagacg atcgcggact cgatctccat cgtcgacatc cggtgccccg
     3901 agacgttgat cacgtcgtcg gtgcgcccga cgatccagaa gtagccgtcc tcgtcgatct
     3961 tcgcggcgtc gccgaccacg tacacgtcgc ggccccactt gtcccagtag gtctcgacgt
     4021 agcgctcgtc ctccttgtag agcgtgcgca gcatgccggg ccacggccgg cgcagcgtga
     4081 ggaagccgcc gccctcctcg acgatctcgc cgtcgcggtt caccaccgcc gcctcgatgc
     4141 ccgggaacac cttcgtggcc gaccccggct tggtctcggt gattcccggc agcggcgtga
     4201 tcatgatgtg gccggtctcg gtctgccacc aggtgtcgac gaccgggcag cgctcgccgc
     4261 cgatgacctt gtggtaccag gcccaagcgc gcgggttgat cggctcgccg accgagccga
     4321 gcaggcgcag cgacgtgagg tcgtgcttct ccgggtactc gtcgccccac ttgatgcagg
     4381 cgcggatcgc ggtcggcgcc gtgtagagga tcgtcacgcc gtagtgctcg acgatctccc
     4441 accagcggtc cttgtcgggg tagtcgggcg cgccttcgta gagcaccgac gtgcagccgt
     4501 tggcgagcgg tccgtagacg atgtacgagt ggccggtgac ccagccgacg tcggccgcgc
     4561 accagtacac gtcggtgtcc ggctcgaggt cgaacacgac cttgtgcgtc gccgccacgc
     4621 cggtcaggta gccgcccgtg gtgtggacga tgcccttcgg cttcgccgtc gagcccgagg
     4681 tgtagaggat gtagagcggg tgctcggcgt cgagcggctc ggccgggcac tcgggctcgg
     4741 cctcctcgag cgcctcgtgc caccagatgt cgcggccctc cgtcatcggg cactcgccgc
     4801 cggtccgctt gaccaccacc acgtgctgga gctccggaag gtcgccgagg atctcgtcga
     4861 ccttcgcctt catcgggttc ggcttgccgc gccgcagggt ctcgtcggcg gtgacgagca
     4921 ccttcgcgtc cgagacctcc atccgctcct tcacggactc gacgctgaag ccgccgaaga
     4981 ccacgttgtg gaccgccccg atccgcgcgc acgcgagcat cgcggcgggg gtctgcggga
     5041 ccatcggcat gtagatcccg accacgtcgc ccgggccgac cccgattccc ttcagcacgt
     5101 tggcaaagcg ctgcgtgtcg gcaaggagct gcgcgtaggt gaggtcgagc gtgagcccgt
     5161 cctcgcccac ccagtggtag gcgaccttgt cgccgttgcc ggccgcgacg tgacggtcga
     5221 ggcagttgtg cgaggcgttg agcttgccct ccttgaacca cttcgcccac ggcgggtccc
     5281 agtcgagcac ggtctcccac ggctcgaacc agtcgagctc cttggcccat cgctcccacc
     5341 acgcctcgaa gtcctcctcg gcctcgtcgt agaccgccgg atccgagaac ttcgcccgct
     5401 tggcgaattc ctccggcggg gcgaagcggt cctcgtcgcg gagcagtacc tcgaggtcct
     5461 cgcgttgttc aggagagtcg ctcatgccag ctctataccg catccacggc gcgcgccaac
     5521 ttcgtgtacg caacgcccgg ttcggggtcc gagaacaccg acgatcccgc cacgaacagc
     5581 cgcgcgcccg acaccgcggc gcgggggccg gtcgtggcgt cgatcccgcc gtcgacctgg
     5641 atcttgatgc cggggtcgag ccgcgtgcgc atgcgctcga tcttgccggt ggtggcctcg
     5701 atgaacggct ggccgcccca gcccgggttc acggtcatgc agagcgcgat ctcgatgtcg
     5761 gaggcgactt cgtcgaacgc gtcgatcggc gtcgaggggg tgatcgcgac acccgccagg
     5821 cagccggcct cgtggatcgc cttgacggcg tagtgcacgt gcggagtcgc ctcgaagtgg
     5881 acggagatcg agtcggcgcc ggcgcgcacg aactcgtcga cgtggcgctc cgggcgctcg
     5941 accatcaggt gcacgtcgag gatgccgccg gcgccgtgca cctggtcggc cagcgcgctg
     6001 acgatcagcg gcccgatcgt gatcgggggg acgaagtgac cgtccatcac gtccacgtgg
     6061 atcacccgcg cgccggcgtc catgacctct cgcacctgcc cgccgagccg cgtgtagtcg
     6121 gcggccagga tcgacggcgc cacccgtcgg tcgcgcagca ggtcggtcgc gacgctcacc
     6181 ggcgtgaagc taccggaacc cgccgcccgg cctccggccg gggcccggaa cgagacgggg
     6241 ccccatcgct ggggccccgt ccgctcggac tgccgctctc ggggagcggc tggggagctg
     6301 cgctagtccc tcttgctgcc cttgtcgttg tcctgctgct tgccctgctt ctggtccttc
     6361 gacttcggtg cggcgcgccg cacgtcctcg caggagccgt tcgcgttctc gggcgtggcg
     6421 tcggcgatca cgtccgccgt gtccagcagc gccaggtcgg tgcccggacc gcagttgacg
     6481 gtgtcctgct cgccgtcgcg gaggtggaag cggtcgtcgc cctcgccacc gaccagcgtg
     6541 tcgccggtcg tgtcgagctg cacgtccctg ttgacgtcac cgcgggcgag cgcccacagg
     6601 tcgtcgttgc ccgggccacc gtccgtggtg tcgacgccca ggttggcgaa gatcaggtcg
     6661 ttgcccgccc cgccgttcga ggtgtcgttg cccctgccgc cggcgatgcg gtcgttgccg
     6721 tcgccgccgt tggtggtgtc ggtcccgtcg ccgccccaca gcaggtcgcg gtgcgcgccg
     6781 ccgtcgagcg agtcgtcgcc gtcgccgccg tggacgcggt cgcggccgtc gccgccgttc
     6841 agcacggcgt cgttgccgtc accgccgagg atcaggtccc agccgctgtc gccgctgacg
     6901 ttgtcgacgc cgctgccccc gcggatgaag tccccgccgg agccgccgct gacgatgtcg
     6961 tcgcccgcga acgcgtagat cttgtcccgg ccgccgttgc cgtcgatggt gtcgccggcg
     7021 ttggtcccgc gcagcttctc gccgcgatcg gtgccggtga tggtcgccgc gaatgccacg
     7081 gccacgagcg tcagcccgag cgtgcccacg acgaccaggg tggtgagaat gcgtgttcta
     7141 cgcatgaggg ggtctgcgcc tccttggtcg cttgatggtc ctaacgcatg ggcggcgcca
     7201 tggttgcggc gtccgcggag ccccgcggta ggctttgcca ccaatgaggg gtagatggat
     7261 ccgctaggag ggtcacgatc atgaccagga aggtcacact cgccgcgttc gcgctggcgc
     7321 tcgtacttgc actcgttccc gctggagggg ccgctgccgc gccccagggc gagcagatca
     7381 gctcggcgtc ggcgttccct ccgctcaccg aggcctccgc gcggcgcctg gcgctgaagc
     7441 tggcgcgctc ggtcgcccac aagcgcaacg tccgctcgtg gcagctctcg gaggccctca
     7501 gcgtgaggcc gaaccgggtg gtcttcgggt acgccgatcg caaccgcaag gaggtcttct
     7561 gcacggcgag gctcgtcgtg gagcgctcgg ccaccaggcg gcgtgccttc atctcggccc
     7621 ccgagtgcgc gggcatcccc ggcgaggcgc tcgagatcga gcgtgccacg agcgcggtgg
     7681 tgcgcgccgt cgccggccag cgccccgacg cgcggctgtc ggtccgccgc tacgagaagg
     7741 acctccgcga ctgcgagggg ctcgttgttc ctcgcggccg ccatgccgag gtcgagctgc
     7801 tctacgacgc gggcgcgacg ctcgcctcgg tcgacccgct gctcacgcac ctcgacgcct
     7861 ttgtcacgcg actccaggac atccagccgg aggacacgga gctggcgagc ggagtcgtgt
     7921 ggtggcgccg gctcatcacc tcgctcggat cgctgccccg cacgacgctg cggccgtgca
     7981 cggccatcct cgagtgggcg aacgacaact actcgaccga caccgccccg gtggacttcg
     8041 cggagctcca gcgcacgctg gacacgctga agcgtgagaa ccgcggcatc ctgcgggcgg
     8101 cggagcatct ggccgagctc ggggtcgccc cgcggctgtc ggccggattc acgcccgacg
     8161 tgctgatagc gacggccctg ggtcgttaga tccggacggg cgtgtccggt tggggcacgc
     8221 ccgtacgttg tagagggtga tggacttcga cagcctctac gacgagcttc gcgacccgct
     8281 cacgcgcagg atcgagcgcg tcgtcggcga tccgcgcacc gcggaggacc tgcgccagga
     8341 ggcgttcgcc cgggcgtgga ccagcgcccc acgtgacgcg ggccacggcc agatgcgcgc
     8401 ctggctgcac cggaccgcac acaacctcgc cgttgacgag ctgcgccgcc gccgcgtgcg
     8461 cgactgggtg ccgtacgacg acgagctggc gccggccttc gaggacggcg acgccgacga
     8521 gcgcatagcc gcacgcgagg cgctcgccgc gctcacgccg cacgaccgct tcctgctgct
     8581 gatgcgcttc gagggcgggc tctcgcacgc cgagatcggg cagttgctgg ggatcggcgc
     8641 cgacgccgcg cgcaagcgcg tggcccgggc gcgcaccgcg ctggcggcgg cgcaccgcaa
     8701 ggtcaccccg cgcgagcgcc cgctcgtcct ggtgctggtc ggcgacgagg acctggcccc
     8761 gtacgagagc tggatcaacg cggccggcgg cgaggcgcgc ccgctcgaca cgacccgctt
     8821 cgagcgccag ctcgcctcgg cggacgcgct cgtgatcacc ggcagcgaaa ccgacatcca
     8881 tcccggcctg tacggcgagc ccgtgcgggg ttcggtcggc gacctggacc tcgcgcggga
     8941 ccgccgtgac ctggccgcgg tgcgcgcggc gctgctccag gacgtgccgc tcgtcggggt
     9001 ctgccgcggt caccagctcc tgaacatcgc gctcggcggg acgctgcacc aggacatcgc
     9061 gcggacgccg catacggacc ttggcgggcg ccgcgggcgc gcgctctgcc accgcgggga
     9121 ggagcacccg atccggaccg gctcgcgcac gctcgcgcgg cgcgtcctcg gtcgtggcgc
     9181 gacggttgcg agctgccacc accaggcggc gaagcgggtc ggcgacggcc tgcgcgcgac
     9241 gtccgtgtcg cccgacggcg tggtcgagac gctcgagctt ccgcagcggc gcttcgcgat
     9301 cggcgtgcag tggcacccgg aggtcggcgg gagcgccagc gagagcctgg catcggcgct
     9361 cgtggaggcc gcgcgatgag cggggcgctg gggattcggt gggcctgcat gcacgacgcg
     9421 ccggcgctgg cgcggctcgc cgcgctcgat tccgcggcgc cgctgcgcgg cgaggtgctc
     9481 gtggcggagg tcgacggcgt gctgcgggcg gcgctggcgc ccgcggaggg gcgggcgatt
     9541 gcggacccgt tcgagccgac cgcggagctc gtggcgctgc tgcggcttcg ggcggagcag
     9601 gcggcgcgcg cggagccgcg ccccggccgc ctccggcgct tgcgctctcc gcccgcgccg
     9661 caaacggcga gcacctaggg cactgggtcc gagccccggc gtctccgaga acgaggtgtc
     9721 cggctttagt ggcgcatagc gccacaaagt acgggcacct cgcgctgggg agccgttcgc
     9781 ggcgcggctc tagaccggct cggcccggcg cgcgtgcgtg cggatctcgc tgaggatccg
     9841 ctccagacgg ggctcgacgt cgtggtcgac gtgcgtcatc gccgccgcca ccgccggatg
     9901 cacgcgcgcg atctgcgcgt gcatctcgtg tgcaaccgcg cggtagccgg ggtggccctc
     9961 gcgacccgag cgcagctcga tcgtgtgcat cgcctcgcgc gcgttcatgt ccatcacgta
    10021 ccggatccgg aagccgaggc agagcgcgta cggcgcgagg tccgccatgc cggcgtcgac
    10081 caggcgctcg tactcctcgc gcgagcggtc gagcccggcg cggtagaggt cggcgacgcc
    10141 cgcctcctcc agctcggcag gcacgcccgc cccgaggtac ggcgtgagcg gctgccactg
    10201 gatcgtgagc aaacgatggc gctgcaggtc gcggaaggcg ccgtagtcgg agacgacctc
    10261 gaagcggtag cggatcgcct cgaacccgcg gccgggccgg tggcgcctgt tcgcgcggtc
    10321 gccgaccagc tcggcgatca gcgccgcgcg ctcgtccggc ggcagcacgc tcacggccga
    10381 gcgcagttcc tcctcggaca cggcggcctg ttcgaagagc atcgcggcca ggaggtcgtc
    10441 ctcgccgccc tgcacgtcga gcaaccgcac cgacggcccc tcggcgtcct cctcgcgtgg
    10501 gcggtcgagg cccatgcggt gggcgagccg gcccgcggcc tcgcgccgct cgccgatcac
    10561 ccggatccac tcgccgccgc gctcggggcg atccacccgc gagacgaagc tcggcatcat
    10621 cgccttcagc tcggtgagca ccatcgcgcc cacctcgcgc gcctccggca gcggcgacgc
    10681 cagcaggcgc atcacgagct gctcgtaggc ctgcccggtg gcgaagatcc ccatgtgcga
    10741 gagcgacgcc gccggcagga ggccgcgcag gaggtcgagg gccttcgcgc ggatcgagcg
    10801 ccggtgggcg gcctcgggct cgccctcgga gcgcgggaag cgctcgtccg cccaggcctc
    10861 cacccgcggc agcgcctcgg cgtacgcatc gaacagcgcg tccatcgcgc gggcgtactc
    10921 ggggccgagc gagtcgtcgc gccagtagcg gtagccgccg cccggcatcg cgccgtcgta
    10981 cgggatgtag cgggtggact gctcgaggta ggccgcgagc cggccgcgct ggagcacctt
    11041 ggtcagcacg ttcgagaccc attcgcacgc gatgtgcgcc ccgccgagct gggccacgga
    11101 gtcgtcgccg tagccgacga agacgcgctc gtagagcttg gccgcgcgct cgccctcggc
    11161 accgtcgtac ccaccgccgc ccgacgcgac gtcgtcggcg aactcctcca ggtaaagccg
    11221 ccgcagcgtg cccgggtagc gcgagtagcg ggcgaacagg gcgcccttga cggtctccgg
    11281 caggttcacc aggccgaaca ccggccggtc caggttggtg aagtgaggcg ccagccggtc
    11341 gcgctcctcc tccgtgaatc gttcgaccgg gtagtccatc ggcggcgcga gtctagaccc
    11401 ggccccggcg gctcgcgtga cggccgaccg gccgccggtc agtggacgcg tcagcccttg
    11461 ccgtcgtggc cgaccgtgac ggcgccgccc tcgacgatcg tgggcaccac gccgcgcggc
    11521 gagtacatga acagctcgct cttccactcg gcgttggtgg tcgcgtcgcg ctcctcccac
    11581 tcgatcccgt gctggcgcat ctggccgcgc gcggcctcgc agtaggggca ctcgggcttc
    11641 acgtacatga tccggtcggc gatggtctga gagtaccgtt cgccgggtgg cggtcgagac
    11701 cgagctgcgc gaggtgggca gggcactcgc ggcggcgttc ccgtcgaacg cccggcgccc
    11761 cctgcgggcg ctcgacgagc gtgcgatgga gctcacgacg cgcgaccagg agctgcgcgc
    11821 cgcgctgttc cggctcgtgg acgtgacgcc cgcctgcaac tcgctcgacg agctcgcgcg
    11881 gcacctgtcc ggctacctgg aggacgtgga cgagcgtccg cagccgctgc gcgccgcgat
    11941 gcggatggcc gacagcgctc cgggccgccg cgcgctcggc gccgccgccg cggccggcgt
    12001 gcggcacatg gcccatcgct tcatcgtggc cgagtccccg aaggccgcgc ggcgcgagct
    12061 gcgcacgctg tggggcgagg gcatcggcgc ctcgctggat ttgctcggcg aggcgaccgt
    12121 caccgccgag gaggccgacc gctacgccgc gcgctgcgcc gacgcgctcg acgtgctcgc
    12181 cgcggacacc gcgcgctggc ccgagcgacc gctgctcgag cacgactcgg ccggaccgat
    12241 cccgcgggcc aacctgtccg tgaaggtctc cgcgctcacg ccgctgatgc gtcccgagtc
    12301 gccggagcgc gggcgcgacg acgccgcgcg gcggctgcgc cccctgctcg accgcgctcg
    12361 cgacctcggt gcgcacctgc acatcgacat ggagtcggtc gacgccctcg agaccacgct
    12421 cgagctggtg gtggacctgc tcggcgagga tgctttccac gacgggccgt ccgcgggcgt
    12481 ggtgctccag gcgtacctgc gcgactcggc cgcggagctc gaccgcatcc tcgactgggc
    12541 ggagcgctcc ccgcgcgcca cgccgctcgt ggtccggctc gtgaagggcg cgtactggga
    12601 ccacgaggtc gtggaggccc gccagcacgg ctggacgccg ccggtgttcg aggacaaggc
    12661 cgagtgcgac cgcaacttcg aggcgctcac gcggaggctg ctcgaggcgc ggccgctcgt
    12721 gcgggtcgcg atcgcctcgc acaacctccg ctcggtcgcg aacgcgatcg cgaccaaccg
    12781 cgagctgggc ggcgacgacg ccgacctcga gctccaggtg ctgcgcggcc tgggtgacga
    12841 cctcgccgac gcgctcgcgg ccagcggttt ccgcgtgcgc tcgtactgcc cggtgggcga
    12901 gctggtggcc ggcatggcgt acctggtgag acgcctcctg gagaacaccg cgaacgagtc
    12961 gttcctgcac gtgcaggcga gcggcgcgtc gctcgacgag ttgctggcgc cgccctgagg
    13021 ccgttcgcga acgagccgac gcttgagctg cgccgcgcgc ccgcgcgcga gtcgctgctg
    13081 ggggcgctgc gcgagctgga gccgcgcctg ccgctgcgcg tgcccgtgct ggtcggcgac
    13141 ggcgcgcgcg ggcccggcga ggccgccggc ggagccggcg cgtccggcgg ggggtccgcc
    13201 tccggcggcg accccttcga gtccaccgac ccgggtgagc ctgatcgcgt cgtggccgtc
    13261 gccgcccgcg ccaccgaggc cgacgccgac gccgccgtcc gcgaggcagc ccgcgccttc
    13321 ccggcgtggc gcgacctcgg tgcgcccgcc cgcgccgagg tgctcgtcaa ggccgccgcc
    13381 atcctccgcg agcgccgcct cgagctgtcg gccctccagg tgcgcgagtg cgcgaagccg
    13441 tgggccgagg cggacgcgga cgtgtgcgag gcgatcgact tcctcgagta ctacgcgcgc
    13501 gaggcggtcc ggctggccga gggccccgag ctgctccagg cccccgggga gcgcaacacg
    13561 atgcgctggg agccgcgcgg cgtggtggcc gtcatctcgc cgtggaactt cccgctggcg
    13621 atcccgatgg gcatggtcgc cgccggactc gcgacgggca acgccgtcgt gctgaagccg
    13681 gccgagcagt cgcccggctc cgcgctgatg ctcgtggagg cgctgcgcgc ggcgggggtc
    13741 ccgccgggcg ccctcgcgct gctgcccggg ttcggcgacg tgggcgcggc cctggtgcgc
    13801 cacccgggcg tgcacacgat cgcgttcacc ggctcgggca aggtcggcct cgagatcgtc
    13861 cgcgccgcgg ccgagacccc cgacggccag ggccacgtga agcgcgtgat cgccgagatg
    13921 ggcggcaaga acagcgtgat cgtcgactcc gacgccgacc tcgacgaggc ggtccccgcg
    13981 ctcgtccgct ccgcgttcgt ctacgcgggc cagaagtgct cggcggccgc gcgcgtgctc
    14041 gcgcacgagc ggatcgccgc cacgctggtc gagcggctcg ccggggcgac gtcggtgctc
    14101 gaggtcggcc aggccgaggg cttcgggatc gacgttccgc cggtgatcga gcgtgaggca
    14161 cgcgaccgcg tggagggcta cgccgagcga gccgccggcg aaggccggat cgcggcgcgc
    14221 gccgacgcgc cccggggcgc gggctggttc gtcgcccccg ccgtggcggc cgagctgccg
    14281 ccggactccc cggtgctgac cgaggagatc ttcggcccgc tgctcgcggt cgagcccgtg
    14341 ccgagcgtga gcgccgcctg cgacctgctc gacgcctcgc cgttcgccct caccggcggc
    14401 ctcttctcac gcgacccgcg cgcgatcgag gaggtctcgc ggcgcacgcc ggtcggcaac
    14461 ctctacgtga accgccacat caccggagcg atggtcggcc gccagccgtt cggcggcaac
    14521 cgccgcagcg gcgtcggctt caaggcgggc ggaccgggct acctgctcca gttcgtcgaa
    14581 ccgcgcgtta tcaccgagaa cacgatgcgc cacgggcttg tcgtcgagta ggcggctggc
    14641 cgaggcggca cgcttccgtg cgaggctcgc cgccggcacg gcgctggacc tgctggacgt
    14701 catcgccggc cgcggcgacc cgctcgcgcc gccgcgccgc ctgctcggcg acgactacag
    14761 cgagttcgag cggatcggga acgagttcct cggccacttc gtgaagctcg gcggcctcga
    14821 gccgcaccac cgcgtgctcg acattggctg cggcctcggc cggatggcgg tcccactcac
    14881 gcgctacctc accacggccg gcagctacga gggcttcgac gtcgccaggc tcgagatcga
    14941 ctggtgccgc cgcacgatcg gctcgcgtca cccgaacttc cagtttcgcg tgctcgacgt
    15001 gcgcaacgag cgctacaacc ccggcggcag cgcgcagccc acggcctggc cgttcgccgg
    15061 cgccaccttc gacttcgcgt tcgcggcatc ggtcttcacg cacctgcgtc cggacgacgc
    15121 gtccgcttac atcgccgagg ccgcccgcgt gctgcgcccc ggcggcaccc tcttcgcgac
    15181 cttcttcctg ctcgacgagg agacccgccc gcggatcgag cgcggagaca gccacttctc
    15241 gttccgcgcc gcgaccgacg gtcccgccgc cgcccaggac gccggcagct tcgaggccgc
    15301 cgtcgcctac gacctcggct gggtccgcga ccgcctcggg agcgccggcc tggacgtgac
    15361 cgacctgcgc cacggctact ggagcgccgc cgacgaccac ctcacctggc aggacgtgat
    15421 cgtggcgcgc cgccgctagt acccgacctg gtgcgtttcg ccgcgttgca ccgggtacgt
    15481 tgcctgccgt gccccagctc gtccttggac ccctcctgcg ctacgtcggc gagaccgagg
    15541 cgaccctgtg ggtcgagacc agcgagccct gcgaggtcgc gatcctcagc tcccggacgc
    15601 cgacgttctg cgtgcgcggg cgccactacg cgatcgccta cgtcaagggc ctcgagccgg
    15661 gctgccgcta cgagtacacc gtcgagctcg acggcgaggc cgtctggccc gaccccggct
    15721 cggccatgcc ggcgccgtcg atccgcaccc ccaaggaggg cgagcgccac aagctgatct
    15781 tcggctcctg ccgggtcggc tatccgcacg agccgccgta caacctcacg aaggacgaga
    15841 gcccgctcgg ccgcgaggtc gacgccctcc gcgcctacgc cctgcgcatg cgcgaccttc
    15901 cggagagcga gtggccgcac ctgctgctga tgatcggcga ccaggtctac gcggacgagg
    15961 tctcgccaca cgtcaagagc ttcatccgga accgccgcga caccaccgag ggggccggcg
    16021 agcaggtcgc cgacttcgag gagtacgtgc ggctctacca cgagtcgtgg ggcgagccgg
    16081 tgatccgctg gctgctctcg acggtctcga ccgcgatgat cttcgacgac cacgacgtgc
    16141 acgacgactg gaactcgtcc caggcgtggc tcgacgaata ccgcgccaag tcgtggtgga
    16201 aggagcgcat cgccggcgcg ttcatgtcgt actggctcta ccagcacctc ggcaacctct
    16261 ccccgcgcga ccatgacgcc gacgagctgc tccagcgcgc gcgccgggtg cccgacctcg
    16321 gcgaccacct gcacgagttc gccctgcgcg ccgaccgcga ccccgccgcc caccgctgga
    16381 ctttctgccg cgacgtggcc aaggcgcgcg tcgtggtggt cgactcgcgc gccgggcgcg
    16441 tgctcgacgg cacgcgcaag atgctcgacg acgacgagtg gcgctacctc gaggagcacg
    16501 cgcgcggcgg cttcaaccac ttgttcgtgg ccagctcact gccgttcctg ctgccacgcg
    16561 cggtgcacga cctcgaggcc tggaacgagg ccgtctgcga cggcgcctgg ggccggccga
    16621 tgatcgacgt gggggagagg atcaggcagg ggatcgacct ggagcactgg gccgcgttcg
    16681 gcgactcgtt cgagcgcgtc gaggggctgc tgcgcgcggt cgccagcggc gagcgcggcg
    16741 acccgcccgg cacgatcacg atgctctccg gcgacgtgca ctacgcctac ctcgccgagg
    16801 tgggcttccc gcgcggcacc ggggcgaaat cgccggtcta ccaggcgacc tgctcgccgt
    16861 tccgcaaccc gctcgactcg aaggagcgca ccgcggtgcg cttctcgtgc gggcgcacgg
    16921 ccgaggcgat ggcccgcgcc ctcgcgcgca gcgccggcgc ccgcaagccc gagatcggct
    16981 gggagctgcg cgagggcccc gtgttcgaca accaggtggc gacgatcgag tgggaggggc
    17041 cgcacgcgga gctgatactc gagcgcgcca taccggacgg ctccgtggac ccgcggctcg
    17101 aggagacgtt ccgccggacg ctgtcctaga ggctcgaccg gaggcggggc gcgctcaccg
    17161 ccgcgcgggt tccatcgccc cgaacacggt cgcgagccgc caggcgtagt agcggctcat
    17221 cgtgtcgagc gcggcggctg gcgagccgag caggcgctcg acgcgcgggc ggtccagcac
    17281 ggcccaggcc tggtggtccg gttgggagag gaccgtgtcg cggatctcga ggaggatgcg
    17341 cgcgaagggg tcgtcgggcg cagccgcgat cgcgctgcga cgcgccagcg cccgccgccg
    17401 cgcctcggcg gcggccttcc ccgccagcgc ccgtgcgcgc gccatccggt gcgccgcccc
    17461 ggagcggcgc gcgggccagc cccgaccgtc ctcgaacggc acctcgacca gctccggcgc
    17521 cagctcctcg agcacgcgca ggtggaacag ctcgcgcgcg cgctcggtcg cgggcaggcc
    17581 gagcaggtcg ggcaggaggc gccgcgacca cagcggcgag gtggtgtcgc gcacgaactc
    17641 gacgcacccg tgcgacggcc ccgcccaggt gcccatgcgg cgctggaggt agaacatgtc
    17701 gggcacgtcg agcgcggcca cgccggcgtc gagctggcgc tcgacccacg cggcgatctg
    17761 gtcgcgcacc agctcgcggc cggcgtcgcc gagcacgtcg gtgcggcctg ggcggcggcc
    17821 gaggaacgtg cgcgtgagcc gttcggtgag cgcgtcgggc gccgcgccgt cgcccaggcc
    17881 gtagtaggcg cgggcgatct cgccgccctg ccccgagtgc cacagcacga gcgggccctc
    17941 gcgcgggccg agcgggaagc ccgccgcgtc ggccaggctg gcggtgccgg aggaggtcat
    18001 gtccacgatc gcggccgcgc ggcgccagtc gtcccagacg ctgccgtggg gatcgtgggc
    18061 gaggatcgaa tgcccgatgc cgcccacctc cgccagccgc cggccgacct gcacgtcggg
    18121 cgaggactcg tcgccgcccg tggtggactc gaagtcgacc ccggcgtgga gggccgcgcc
    18181 gagcaccacg cgcgagtcgc ggcccccggt caccgggacg acgttggggc gctgcggcca
    18241 gtcggccagg gcggccagct cggcggtgag caccccggcg gcgcgctcgg cgtcgaaccc
    18301 ggcgccgatc agcggcgcga tctgcccgag cgggagcagc ggcgcgctgg ccgccggccg
    18361 ccccggctcg aggcgccgga cgcagccgta ggtcagccgc cgcacgccgc tccagaccgg
    18421 gtcgcccgac agcgaccagc ccccgccgag caccgtcgcc agcaccgcgg ggtcgagctc
    18481 gcgtccgtcg atcagaccgc gcagcagctc cgcgttgttc gaagtccagc gcaccccgcc
    18541 ggcgtgggcc tcgtagagcg ggtacgcgcc gagtgcgtcc gagaccaccg cgagcgtgcg
    18601 gtcggcgtcg tcgtagcgaa gcgcggcgaa gcggccgtcg agcgccgggg cccaggcgtc
    18661 gggctgcccg aggtagaagg cggggtcgag cggtgcccgc ccgtcggcct cgcgctcgcc
    18721 ggtccagcgg atcgggcgcc ccgagaacag cgcgaggcgc gccggctcgg tggcggcgta
    18781 ctcgatccca cccgtctcct ccggcccgtg tgtgacccac gctgccccga gggatcccga
    18841 cggtgccgcc cacgtctcga cgggccgccc cgggaagaac ggcatccgcg cgagcagccg
    18901 ccggagcgcc tccgcggcgg ccccgggctc gacccctccg tcccccgacc agccgaccag
    18961 gaacagattc accgcggaag tgttacgcgc ggcgcaaggt cggcgagggc gatgtgcatc
    19021 attgccgggt gctcaaccgg ctcctcccct cgctggcggc gttctcccgc ggcttcgccc
    19081 tggcctcgcc cggcggcgac gtgcacgagg cggacggcgt gatggccgcg atcgtgcccg
    19141 agctggccga tcgctcggtc gtgaactgcg tcgtgtacga ccacgcggcg tcgctggggg
    19201 cgcggctcgg cgcgatcgcc gcggcctacg acgaggccgg cgtgcgggcg tggacggtgt
    19261 ggaccccgga gagcgaccac gcggcgatcc gcacgctcga ggccgcgggc aacgtgctcg
    19321 acgcggagcc gacggcgatg gggcgcgagc tcgacgggat cgaggcgccg ggcgccggag
    19381 agctcgacct gctgccggag cccgcgccgg gcgacgtggc ggacgtgctc gcgcccgtct
    19441 acgggtggga caccgccgcg cgggccctgg tgcgctggca ccccggctac cacccgtacc
    19501 tggcgctcgc cgacgggagg cccgccgtca cgctcgcggt gcatgaccac gacggcgacg
    19561 cgcacgtcac gctcgtcggc accctggagg ccgcgcgcgg gcgcgggctc gcgacgctgc
    19621 tgctgcgccg cgcgctcgcg gacgcccgtg accgcggctg caccaccacc acgctcgtgg
    19681 ccacgaagat gggccgcccc gtgtacgcgc gcctcggcta ccgcgacctc ggccgcatcc
    19741 agatgtggga gcgccgcaag ccggacaccg ccgacgccgc gtgagcgcac tgcgcgcccg
    19801 cccgcccgtc gccctggcga tcctcctgct gctgggggtc gccgcacgcg tgtggctgat
    19861 ggtcgactac cgcccggcct acctgtcgag caacgactcc gcgcgctttc tccacttcgc
    19921 acacatcgac gacggcatgt tcgaggacac cttcggcccg accgggtacg cggcgttcct
    19981 gaaggtcgtg cgcgtcgtct cggaccagat cgagttcacg atcgccctcc agcacctgct
    20041 cggcgtgctc gcggcggtgc tgctgtacgc ggcgctgcgg cggctcggcg ctccggtgtg
    20101 ggccgcgtgc gtgccggccg gcgccacgct cctctccggc gaccagctct acatggagca
    20161 cacgctcctg tcggagggac cgttcctgcc cctgctcgcg ggcgggctct acgcgggggt
    20221 gcgcgcgctg gagggcgagc gctcgatgcg ctggctcgcg ctcgccggcg tgctgttcgc
    20281 cggcacggcg ctgtttcgca acgtcgggct gatcctgccg ccggtactgg tggcgtgggc
    20341 gttcgtcgcg atgccggggg gcgtgcgcgg gaggctcagg tcggcggcgg tcgcggctgg
    20401 cgcggcggcg gcggtgctgc tcgtctacgc ggcgatcgcc gggctcgacg gcggcaacac
    20461 cggctggacc gcgaccagcg gctggaacgc gtacggccgc tcggccccgt tcgccgactg
    20521 cgaccggttt gatccacccg ccggcaccga ggagctctgc gaggacacgc cgcccgaccg
    20581 gcgccccggc tcgctgttct acctctggtt cgacggctcg ccggcgcgcg cgaagttcaa
    20641 gcatccgccg ctcgggcagg agaccctcgg caagttcggg cgccgggcga tcctcgcgca
    20701 gccgctcgac tacgtgaaga cggtcgccgc cgacctgccg cgcttcctca accccgagct
    20761 gaacaagaag cgctactcgg gaggcgggta cttcctgatg tcgcgtcgcg gcgacgtggc
    20821 cgagcggaac gtcctctacc agctctcgcg cgactacgac cacgggaggt tcagcccggg
    20881 ctcgagcggc gacacgatcg cggaatggca gaggctccag cgcctcggcg gggtggtgcc
    20941 cgggctgttc gtggcgttcg ccctgctcgg cctggccgcg gcgcgcgggg tggcgctgcg
    21001 gggcctggcg ctgttcggcc tcgcgggcgc gggcctcgtt gtgatgccgg cggccacgct
    21061 cacgctgcta ggccgctaca ccgtgccgcc gatgccgatc gtggccgggg cgggtgcgat
    21121 cggcgcgtgg gctatcgccg cgcgcctgcg tgagcgacgc cacgcgcggc gcggcccggc
    21181 aggggagaat ccgtccccat gaccactttg cggatcctgg tcggcgcgct gctcgtctcg
    21241 acggcgatcc actacacgga caactggctg tcgatcgagc actacgcgcc gcgtgagggc
    21301 ttcgtcgcgg agaacccggg gctcgtgccg gtcgcgtggg tgctgttcac ggtgctgggg
    21361 atcgccgcct accgcgagta ccgccgcgca ccgtcgctgc gcgcccacct catgctcgcc
    21421 ggctattcgc tcgccgggat cacgacgttc gcgcacctcc tctacgaggg gaacgacttc
    21481 gcggcgtggc agtgggcctc cgtgctcgcg gacggcatca ccggcagcgc ggtgctcggc
    21541 ttcgcgctct ggtccgcggc gcgcgtgcgg cccgcggcga ccgtggcgta gagtccgccg
    21601 caccgtgtcg gtagagctcg cactcaagcc gccgctgttc aacccgcgaa cctatgacgg
    21661 cgcggggctc gacgaggagt cgcgccggct gatgctcgcc acgatcgagt tcttcgaatc
    21721 gcgcggcaag gcggtgctca aggagcacga ccgcgacctc acctggtacg cggacttcct
    21781 cgagttcgtg gagcgcgagg gcctcttcgc caagctgctc acgccggcgg ccgagggcgg
    21841 cggggatccc gacaagcgct gggacaccgc gcggatctgc gcgttcaacg agatcacggg
    21901 gttctacggg ctcgcctact ggtacacgtg gcaggtctcg atcctcgggc tcggaccgat
    21961 ctggcagagc gagaacgcgg acgcgaagcg ccgcgccgcc gagctgctcg accagggcgc
    22021 gatcttcgcg ttcggcctgt ccgagaagga gcacggcgcg gacatctact cgaccgacat
    22081 ggtgctcacg cccgacggcg agggcggctt ccgcgcgagc ggcggcaagt actacatcgg
    22141 caacggcaac ctcgcgggca tggtctcggt tttcgggcgc cgcgacgacg tcgagggcgc
    22201 ggacggctac gtgttcttcg cggccgacag ccggcacccg tcgtacgagc tggtgaagaa
    22261 cgtcgtcaac tcgcagtcgt acgtgagcca gttccggctg aacgactacc cggtgcgcgc
    22321 cggggacgtg ctgcatacgg gcaaggacgc gttttacgcg gcgctgaaca cggtgaacgt
    22381 ggggaagttc aacctgggct tcgcgtcgat cgggatctgc gagcacgcgt actacgaggc
    22441 gatcacgcac gccgacgcgc gcatcctcta cggcaaccgg gtcacggact tcccgcacgt
    22501 gcgccgcgcg ttcgtggacg cctacgcgcg gctcgtggcg atgaagctct tcggcgaccg
    22561 ctcgatcgac tacatgcgct cggccacccc ggaggacc
//
LOCUS       DASVPL010000311         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_76806, whole genome shotgun sequence.
ACCESSION   DASVPL010000311 DASVPL010000000
VERSION     DASVPL010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76806"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VF517_17875"
     CDS             complement(<1..1646)
                     /locus_tag="VF517_17875"
                     /inference="COORDINATES: protein motif:HMM:NF009472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEX8054859.1"
                     /translation="MAAERGSVVVVGAGIAGLVATLELLDHGRDVVLLDRCHPEEIGG
                     LAREAFGGMFMVDTEEQRRGGVKDSVELALDDWLRVADFEPGDEWPRRWAEQYVARAR
                     DEVGNWLRTKGVKFFPVVNWAERGVYGDGNSVPRFHITWGCGKGLVDGVWGSIEKHPR
                     RASLEIRTRTRVTGLIEEDGRVTGVRTASEDGGGAEGELRGAAVVVAAGGVGGNLDIV
                     RREWPEELGEAPEHILMGSHYYADGAMHEEVERIGGNVTHLNRMWNYADAVRHPMPKR
                     PLHGLKLIPPRSGLALDPTGRRYGPVPVMPTFDAYFALERMCESDPKYTWLVCNQKIA
                     RRELDVSGSQHNPAIRERRLVRFVLGILLGKPALVQHFVDRCPDFVTGRTLPELAARM
                     SEVTGDGVLDAGLMEREVRRYDDMLARGKSLWNDDQVRRIASLRNWRGDRLRTAKFQP
                     ITDPDAGPMIAIRLQPMSRKSLGGIQTDLGARVLREDGSAIDGLFAAGEAAGFGGGNL
                     HGKRSLEGTFLGGCVFSGRIAAQTIAGAAAEPVRPARPAYG"
     gene            1673..2029
                     /locus_tag="VF517_17880"
     CDS             1673..2029
                     /locus_tag="VF517_17880"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX8054860.1"
                     /translation="MAELTVYEKPTCTTCRKLSALLTERGIDFERVDYHVEPLPEEKL
                     RELVARAGVPARELLRSKEPGGKELREREAPDDEVIAAMAANPALLERPVIERGDRAV
                     LGRPVERALELLDGGG"
     gene            complement(2026..3003)
                     /locus_tag="VF517_17885"
     CDS             complement(2026..3003)
                     /locus_tag="VF517_17885"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8054861.1"
                     /translation="MAERTAFVTGGSGFIGGRLIRRLVRDGWSVRALARSERSAAAVE
                     AAGADSVLGNLDNGGAILDGAGGCDVFFHAAAFVGEWGSRADYERGNVDGTQNAIEAG
                     YSAGVRRFVHVGTEAALLAGDPLVNVDETAPLRPDSRADYARTKARAEQIVRDFDRLE
                     TVVIRPRLVWGVGDTTILPGVEKAVRAGRFSWIGGGRHRTSTTHVDNVVEGLVLGAER
                     GRPGEAYFVTDGEPVVFREFITELLATQGIEVPDRNAPRAVAGALAAAGETAWRLLPL
                     RGGPPVTRMAFWLSALETTIDISKARRELGYEPVVTRAEGMEELRRAAG"
     gene            3040..>3848
                     /locus_tag="VF517_17890"
     CDS             3040..>3848
                     /locus_tag="VF517_17890"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX8054862.1"
                     /translation="MTTATRVDEQRRALPDAPGVYLFRDSKRRVLYVGKASSIKKRVA
                     SHFSNPSTKGARGMLDEIANIEFIATETESEALLAEQNFIRQYRPRYNVRLRDDKSYP
                     YIGISLDEEFPRVYFTREKHRRNRAYFGPFSNAKRVRETLDLLGKVFQHRSCDGVEPG
                     RASGSPCLDYFIKRCGAPCVGYVSSEEYRESIDSIVAFLSGRYRQIERDLEHLMQEAA
                     AEQEFEQAALLRNRLKAVRSLLERQRIANEAVGTLDAIAVAAEGSDANAQV"
BASE COUNT          550 a         1503 c         1290 g          505 t
ORIGIN      
        1 ccgtaagcag gacgcgcggg ccgcaccggc tccgccgcag cgccagcgat cgtctgcgcc
       61 gcgatccggc cgctgaagac gcagccgccc aggaacgtgc cctccagcga gcgcttcccg
      121 tgcaggttcc cgccgccgaa gccggccgcc tcgccggcgg cgaacaggcc atcgatcgcc
      181 gacccgtcct cgcgcaggac ccgggcaccc aggtcggtct gaatcccgcc gagcgacttg
      241 cggctcatcg gctggaggcg gatggcgatc atcggccccg cgtcgggatc ggtgatgggc
      301 tggaacttcg ccgtccgcag ccggtccccg cgccagttgc gcagcgaggc gatgcgccgc
      361 acctggtcgt cgttccaaag cgacttgccg cgcgcgagca tgtcgtcgta gcggcgcacc
      421 tcgcgctcca tcagcccggc gtcgagcacg ccgtcgccgg tcacctcgct catgcgcgcg
      481 gccagctccg gcagcgtcct gccggttacg aagtcgggac agcgatcgac gaagtgctgc
      541 accagggcgg gcttgccgag caggatcccc agcacgaagc gcaccaaccg ccgctcgcgg
      601 atggccgggt tgtgctgcga gccggacacg tccagctcgc gccgcgcgat cttctggttg
      661 cagacgagcc acgtgtactt cgggtcgctc tcgcacatgc gctcgagcgc gaagtacgcg
      721 tcgaaggtgg gcatcaccgg gaccgggccg tagcggcggc cggtcgggtc gagcgccagc
      781 cccgatcgcg gcgggatcag cttcagcccg tgcagcggcc gcttcggcat ggggtggcgc
      841 accgcgtcgg cgtagttcca catgcggttc aggtgcgtca cgttgccgcc gatccgctcg
      901 acctcctcgt gcatcgcgcc gtcggcgtag tagtgcgagc ccatgaggat gtgctccggc
      961 gcctcgccca gctcctccgg ccactcgcgc cggacgatgt cgagattccc gccgaccccg
     1021 ccggccgcga ccacgaccgc cgcaccgcgc agctcgccct cggccccgcc gccgtcctcg
     1081 gacgcggtgc gcacgccggt cacgcggccg tcctcctcga tcagcccggt cacccgcgtg
     1141 cgcgtgcgga tctcgaggga ggcccgccgc gggtgcttct cgatcgaccc ccacacgccg
     1201 tcgaccagcc ccttgccgca gccccacgtg atgtggaagc gcggcacgga gttgccgtcg
     1261 ccgtagaccc cgcgctcggc ccagttgacg accgggaaga acttcacgcc cttcgtgcga
     1321 agccagttgc cgacctcgtc gcgcgcccgc gccacgtact gctcggccca gcggcgcggc
     1381 cactcgtcgc cgggctcgaa gtccgcaacg cgcagccagt cgtcgagcgc cagctcgacg
     1441 ctgtccttca cgccgccgcg cctctgctcc tccgtgtcca ccatgaacat cccgccgaac
     1501 gcctcgcgcg cgagcccgcc gatctcctcg gggtggcagc ggtcgagcag caccacgtcg
     1561 cggccgtggt cgagcagctc gagcgtcgcc accagcccgg cgatgccggc gcccaccacc
     1621 acgacggatc ccctctcggc ggccatccgc gcgcagggta taccttcgtc ccatggccga
     1681 gctcaccgtc tacgagaagc ccacctgcac gacctgccgc aagctctcgg cgctcctcac
     1741 cgagcgcggg atcgacttcg agcgggtcga ctaccacgtc gaaccgctgc cggaggagaa
     1801 gttgcgcgag ctggtcgcga gggccggggt cccggcgcgc gagctgctgc ggagcaagga
     1861 gccgggtggg aaggagctgc gcgagcggga ggcgccggac gacgaggtga tcgccgcgat
     1921 ggccgccaac cccgcgctgc tcgagcgccc cgtgatcgag cgcggtgacc gagccgtgct
     1981 cggccgcccg gtggagcgcg cgctcgagct gctcgacggc ggcggctagc ccgccgcccg
     2041 cctcagctcc tccatcccct cggcccgcgt gaccacgggc tcgtaaccga gctcgcgacg
     2101 cgccttcgag atgtcgatcg tcgtctcgag cgccgacagc cagaacgcca tgcgcgtgac
     2161 cggcgggccg ccccgcaggg gcagcagccg ccacgcggtc tcgcccgcgg cggcgagcgc
     2221 cccggccacg gcgcgcggcg cgttgcggtc gggcacctcg atgccctgcg tcgccagcag
     2281 ctcggttatg aactcgcgga acacgaccgg ctcgccgtcg gtcacgaagt acgcctcgcc
     2341 cggccgcccg cgctcggcgc cgagcacgag gccctccacg acgttgtcga catgcgtggt
     2401 cgacgtgcgg tggcgcccgc ccccgatcca cgagaagcgc ccggcccgga ccgccttctc
     2461 gacccccggc aggatcgtcg tgtcgccgac tccccagacc agccggggcc ggatgaccac
     2521 tgtctcgagc cggtcgaagt cgcgcacgat ctgctcggcg cgcgccttcg tgcgcgcgta
     2581 gtcggcccgc gagtccggac gcagcggcgc cgtctcgtcc acgttcacca gcgggtcgcc
     2641 ggccagcagc gcggcctcgg tgcccacgtg cacgaaccgc cgcacccccg cggagtagcc
     2701 ggcctcgatc gcgttctgcg tgccgtcgac gttcccgcgc tcgtagtcgg cgcgcgagcc
     2761 ccactcgccg acgaacgcgg ccgcgtggaa gaagacgtcg cagccgccgg cgccgtcgag
     2821 gatcgccccg ccgttgtcca ggttcccgag caccgagtcg gcgccggccg cctcgaccgc
     2881 cgccgccgag cgctccgagc gcgcgagcgc ccggacggac cagccgtcgc gcaccagccg
     2941 gcggatcagc cgcccgccga tgaaccccga tccccccgtg acgaatgccg ttcgctccgc
     3001 gatccgcgca gcctaaaggc ccagacctat cctcgaagcg tgaccaccgc gacccgcgtg
     3061 gacgagcagc gcagagccct cccggacgca ccgggcgtct acctgttccg cgactcgaag
     3121 cggcgcgtgc tgtacgtcgg caaggccagc tcgatcaaga agcgcgtggc ctcgcacttc
     3181 tcgaacccga gcaccaaggg cgcgcgcggg atgctcgacg agatcgcgaa catcgagttc
     3241 atcgccaccg agaccgagtc ggaggcgctg ctcgccgagc agaacttcat ccgccagtac
     3301 cgcccgcgct acaacgtccg gctccgcgac gacaagtcct acccctacat cgggatctcg
     3361 ctcgacgagg agtttccgcg cgtgtacttc acgcgcgaga agcaccgccg caaccgcgcc
     3421 tacttcgggc cgttctcgaa cgccaagcgc gtgcgcgaga cgctcgacct gctcggcaag
     3481 gtcttccagc accgcagctg cgacggggtc gagccaggcc gcgcgtccgg cagcccctgc
     3541 ctcgactact tcatcaagcg ctgcggcgcc ccgtgcgtcg gctacgtgag ctcagaggag
     3601 taccgcgagt cgatcgactc gatcgtcgcg ttcctctcgg gccgctaccg ccagatcgag
     3661 cgcgacctcg agcacctgat gcaggaggcg gcggccgagc aggagttcga gcaggcggcc
     3721 ctcctgcgca accggctgaa ggcggtgcgc agcctgctgg agcgccagcg catcgcgaac
     3781 gaggcggtcg gcacgctcga cgcgatcgcg gtggccgcgg agggctcgga cgccaacgcg
     3841 caggtctt
//
LOCUS       DASVPL010000312         9023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_348546, whole genome shotgun sequence.
ACCESSION   DASVPL010000312 DASVPL010000000
VERSION     DASVPL010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348546"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1272)
                     /gene="hisS"
                     /locus_tag="VF517_17895"
     CDS             complement(49..1272)
                     /gene="hisS"
                     /locus_tag="VF517_17895"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX8054863.1"
                     /translation="MPAGRLQAPRGTFDVLPEDGARRLALRRLTDDLLSGYGVVETPV
                     FESTELFERGVGDTTDIVEKEMFTFDDQGGRSVSLRPEGTAGVCRAYLEHGMHKLPQP
                     VKLRYWGPFFRYEAPQAGRYRQFTQVGIEAIGSDDPSLDAEVILLLAELLEKARVRGV
                     RLRISSLGTPESRRAYSDELRAHLREREDQLSDDVRGRLDRNPLRAFDSDHPGTQAAI
                     ADAPRLLDRLDEDDAAHFAAVRALLDAAGQQYEVDPTLVRGLDYYTRTVFEFSSDALG
                     AQSQLGGGGRYDGLIEQLGGAPTPGVGWAAGIERILLAGDLAADEPGTHVFVAGTAGT
                     GKSFALLRFLVGAGMSAEMEQAGRSLKGQLKQANRLGAAYTVIVGDDGFEVKDMATGE
                     QRAVDSPEAVLEAVR"
     gene            complement(1259..1900)
                     /locus_tag="VF517_17900"
     CDS             complement(1259..1900)
                     /locus_tag="VF517_17900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8054864.1"
                     /translation="MDVRMFTVGPVAENCFIFRRDGSDRALIVDPGDEAPKLLKAIEE
                     LGVTLDGILLTHTHFDHVGAVAEVARATGAEVWVPEIEKPVLADINRFQPFPGIGPFE
                     SYDAEHTVAGGEQLKLAGFDIDVIFTPGHSPGHVTYSIADEQALFSGDVLFQGSVGRT
                     DLPGGDWPTLLESIRKLIDSHPEETAVFPGHMGTTTLGRERATNPFLVELARG"
     gene            complement(1910..2266)
                     /locus_tag="VF517_17905"
     CDS             complement(1910..2266)
                     /locus_tag="VF517_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054865.1"
                     /translation="MAQTKRKRRRKHRGTQAGTVEARGRTSRPRTAADAKQVRQQRRD
                     ERLDTPPSWRSSLNRAVLSAIAFGVLAVLVLKVPVASAIALTAVMVLLYLPLSYYTDR
                     FIYNRRQRRKPAGRGR"
     gene            complement(2392..3264)
                     /locus_tag="VF517_17910"
     CDS             complement(2392..3264)
                     /locus_tag="VF517_17910"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054866.1"
                     /translation="MQLLAVPNVSEGSDRAAIAAIGDAFAPARVLDVHSDPDHGRSVF
                     SLAGEQGELAAALAAGARAARERIDLREHTGRHPHVGALDVAPVVYLDHEQRGAACAE
                     ALTAAALIGDAGLPVFLYGDLATAPGNRERANLRAGGTTTLAERVASGDLAPDYGPAH
                     VDPAAGAVLVTARPPLIAFNVDLATDDLDLAQAIAADLRESNGGAPGVRAIGLHLPHR
                     NRAQVSFNVHDHRAAPLRDLIAAVAARAPVAEAELVGLAPAEALDGLPGDLNLRGFDL
                     DRHVIENALRSPQS"
     gene            3283..3356
                     /locus_tag="VF517_17915"
     tRNA            3283..3356
                     /locus_tag="VF517_17915"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3317..3319,aa:Met,seq:cat)
     gene            3395..3751
                     /locus_tag="VF517_17920"
     CDS             3395..3751
                     /locus_tag="VF517_17920"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX8054867.1"
                     /translation="MPKKPTGTIVVYHPEAVDDFQGIKDKQARKKVLTIVSILRQAGT
                     SITEPHVKKVQGEAKLYELRPGGGKTLLRPLYAQIDAYLVILAIGPKAEVNASGFAAA
                     VKRAKRRAKEEFGVEI"
     gene            3816..4214
                     /locus_tag="VF517_17925"
     CDS             3816..4214
                     /locus_tag="VF517_17925"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8054868.1"
                     /translation="MARKKLTKMKTHGELVADQQLVDPDFNREWERLAISRAVAARVI
                     AYRGDNKLSQRDLAKLLEMPQPQVTRLESGEYHPSNETLSRLASKLGMEFTISIAPAK
                     SEPKQITKRTREHADAMVEAGDSITRFSVA"
     gene            complement(4227..4781)
                     /locus_tag="VF517_17930"
     CDS             complement(4227..4781)
                     /locus_tag="VF517_17930"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8054869.1"
                     /translation="MSVIVIEFITVDGIVSDPDGSLGTPGGGWAFRFGPEAIAGDKFR
                     LDSVLDDGVMLLGRKTWQLFSGIWPGRDDPFSTRMNAVPKLVVTRTLTDASAWANSQP
                     IDGELLDVVRRERRDVIITGSLSVVHALMAEDLIDEYRLLTFPVVLGAGERLFAGSPP
                     LDMECLAAERSGAAVLTRWRRAAG"
     gene            complement(4847..5794)
                     /locus_tag="VF517_17935"
     CDS             complement(4847..5794)
                     /locus_tag="VF517_17935"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8054870.1"
                     /translation="MREEGLVDFDPYRGELVAYCYRMLGSFDEAEDLVQETLLRAWRA
                     RDRYDGARASVRTWLYRIATNACLSALEGRARRPLPSGLFAPSDDPGAPLTPALDVPW
                     LQPFPDARFDAGVRVDVRLALVAALQTLPPRQRAVLILREVLGFSASEVAAQLDTTVA
                     AVNSALQRARGALAETRDVDEVTEPDDPRARAIVDRYVDAFEAADVPGLVRLLADDAV
                     LEMPPVPLWYRGSREYGLFMRRVFDMRGLGWAMRQLTANGQPALAAYAPEPGGGHALH
                     TLQVLTVTGGRVARNVVFAHPSVLAAFGLPERISSDALR"
     gene            5964..6224
                     /locus_tag="VF517_17940"
     CDS             5964..6224
                     /locus_tag="VF517_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054871.1"
                     /translation="MTRTRSRALALVAALALIVAAPAGADSSATETAAITAAKSCSSG
                     WKHAVLPNGHKCLRAGQYCKKSWDSRYHHYGFHCHNGRLKRK"
     gene            complement(6227..6733)
                     /locus_tag="VF517_17945"
     CDS             complement(6227..6733)
                     /locus_tag="VF517_17945"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8054872.1"
                     /translation="MERDELRAVQAPLKERYASEPEAALVTLSASGTLGENVSCTVQS
                     GRALAEAGLHPATGGDGSLLCSGDMLLEALVACAGVTLQAVATSLGIEVASGTVRAEG
                     DLDFRGTLAVDREAPVGFASIRLAFELDTEADEAQLATLRKLTERYCVVYQTLAASPE
                     LTSSFSVA"
     gene            6807..7274
                     /locus_tag="VF517_17950"
     CDS             6807..7274
                     /locus_tag="VF517_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054873.1"
                     /translation="MKRLALAAAAAAALALPAPAGAMIQFDQGIAGARIGNSQAEVQA
                     ALGSPSLVKTGTNEFGPWRQFYFAGGLRVFFQGDEDVTSVTLLGRGDRTTKGIGVGNT
                     EKALKQKHPSLTCKTNSGFRSCYTGDFKPGERVTDFWISKGRITRVVVGIVID"
     gene            7331..7576
                     /locus_tag="VF517_17955"
     CDS             7331..7576
                     /locus_tag="VF517_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054874.1"
                     /translation="MPALTTLLLIVALFATIGIWKQAGATSDEADATEKRACIESAEA
                     RYPAVSVSAFVTRDRSASGPLKLSFVRERQRAVAACD"
     gene            7678..>9023
                     /locus_tag="VF517_17960"
     CDS             7678..>9023
                     /locus_tag="VF517_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054875.1"
                     /translation="MLPWTRSKWFRLAVTGFSTIFVAIVLATSLMAEGSALKRADVEY
                     DAVDCVKDKNTKGRARPATGCKRKKRVVIPIDDKQQISEEELERALDELPRLSGPRGL
                     TGHRGANGRRGPTGPRGTAGVGRAGRRGATGARGRSAQGVRGAEGARGADGERGARGD
                     DGARGAEGEAGPAGPTGETGPAGPQGLQGDTGAQGPQGDTGATGAQGDVGPQGVQGDV
                     GPQGIQGDTGAQGPQGDTGAQGPTGPAGPQGDVGPTGPQGPTGPAGPQGDVGPQGATG
                     ATGPAGPQGPQGDIGPAGAQGPQGVTGATGATGATGAQGPQGDIGPTGPAGPQGNTGP
                     AGPAGPQGTTGATGATGATGATGAQGPQGPQGPQGPQGPTGATGATGATGPPAAKYWA
                     VVDSTGSLARSSAGVNSSRITTGQYRVDFGTDVTQCAYIPQIGGTGTTAPSVGMIHS"
BASE COUNT         1376 a         3171 c         3280 g         1196 t
ORIGIN      
        1 gcaggtcgcc ggcccaggcg gagcggtagc ggttcgcgcg cgggggcttc accggaccgc
       61 ctccaggacg gcctcgggcg agtcgaccgc ccgctgctcg cccgtcgcca tgtccttcac
      121 ctcgaagccg tcgtcgccca cgatcaccgt gtacgccgca ccgaggcggt tggcctgctt
      181 tagctggccc ttgagcgagc ggccggcctg ctccatctcg gcgctcatgc cggcaccgac
      241 gaggaaccgc agcagcgcga acgacttgcc ggttccggcc gtccccgcca cgaacacgtg
      301 ggttcccggc tcgtccgcgg ccaggtcgcc cgcgagcagg atccgctcga tgccggccgc
      361 ccagccgacg cccggcgtgg gcgcgccgcc gagctgctcg atcaggccgt cgtagcggcc
      421 gccgccgccg agctggctct gtgcgccgag cgcgtcgctc gagaactcga acaccgtgcg
      481 cgtgtagtag tcgagtccgc gaacgagcgt cgggtcgacc tcgtactgct ggcccgccgc
      541 gtcgagcaga gcgcgcacgg cggcgaagtg ggcggcgtcg tcctcgtcga ggcggtcgag
      601 caggcgcggg gcgtcggcga tcgccgcctg cgtgccgggg tggtcggagt cgaaggcgcg
      661 cagcgggttg cggtcgagcc ggccccgcac gtcgtcggag agttgatcct cgcgctcgcg
      721 caggtgggcg cgcagctcgt ccgagtacgc gcggcggctc tccggcgtgc cgaggctcga
      781 gatgcgcagg cgcacgccgc ggacgcgggc cttttcgagg agctcggcca ggagcaggat
      841 cacctcggca tcgagcgagg gatcgtcgga cccgatggcc tcgatcccca cctgcgtgaa
      901 ctggcggtag cggccggcct gcggcgcctc gtagcggaag aacgggcccc agtagcgcag
      961 cttcaccggc tgcggcagct tgtgcatgcc gtgctcgagg tacgcgcggc agacgcccgc
     1021 ggtcccctcc gggcgcaggc tcaccgagcg gccgccctgg tcgtcgaagg tgaacatctc
     1081 cttctcgacg atgtcggtgg tgtcgccgac gccgcgctcg aacagctcgg tcgactcgaa
     1141 gacgggcgtc tcgaccacgc cgtagccgct cagcaggtcg tcggtcaggc ggcgcagggc
     1201 gagccggcgc gcgccgtcct cgggaaggac gtcgaacgtc ccccgcgggg cttggagcct
     1261 acccgcgggc aagctcgacg aggaacgggt tcgtcgcgcg ctcgcgcccg agggtcgtgg
     1321 tgcccatgtg gcccgggaac accgcggtct cctccgggtg cgagtcgatc agcttgcgga
     1381 tcgattcgag cagggtcggc cagtcgccgc cgggcaggtc ggtgcgcccg accgagccct
     1441 ggaacagcac gtcgccggag aacagggcct gctcgtcggc gatcgagtag gtcacgtgcc
     1501 cggggctgtg gccgggcgtg aagatcacgt cgatgtcgaa cccggcgagc ttgagctgct
     1561 cgccgcccgc gacggtgtgc tcggcgtcgt agctctcgaa cggaccgatg ccggggaacg
     1621 gctgaaagcg gttgatgtcc gcgaggacgg gcttctcgat ctccggcacc cagacctcgg
     1681 cgccggtcgc gcgcgcgacc tcggccacgg cgccgacgtg atcgaagtgc gtgtgcgtga
     1741 ggaggatccc gtcgagcgtc accccgagct cctcgatcgc cttcaggagc ttgggagcct
     1801 cgtcgccggg gtcgacgatc agggccctgt cggagccgtc gcggcggaag atgaagcagt
     1861 tctcggccac cgggccgacg gtgaacatgc ggacgtccat ggcctcaggc taccggcccc
     1921 ggccggctgg cttgcgccgc tggcggcggt tgtagatgaa gcgatcggtg tagtagctga
     1981 gcggcaggta gagcaggacc atcacggctg tgagcgcgat cgcggaggcc accgggacct
     2041 tcagcacgag cacggcgagc acgccgaagg cgatggcgct gagcacggcg cggttgagtg
     2101 agctgcgcca gctcggcggc gtgtcgagcc gctcgtcgcg gcgctgctgg cgaacctgct
     2161 tggcgtcggc ggcggtgcgc ggccggctgg tgcgcccgcg ggcctcgacc gtcccggcct
     2221 gggtgccgcg gtgcttgcgg cggcgcttgc gcttggtctg ggccatcaga tggtcggctc
     2281 caggtccgcg tgccgctggg gccgcgaaaa tcgagtgggg cgagcccgag gacgacgccc
     2341 cgcgaagatt tgcagcggca tccagcggtg cgggggcttg gagtcggcca tctagctctg
     2401 cggcgagcgt aacgcgttct cgatcacgtg ccggtcaagg tcgaagccgc gcaggttcag
     2461 gtcgcccggc aggccgtcga gcgcctcggc cggggcgagg ccgacgagct ccgcctcggc
     2521 gaccggagca cgtgcggcca cggctgcgat taggtcgcgc agcggggcgg cgcggtggtc
     2581 gtggacgttg aacgagacct gcgcgcggtt gcggtgcggg aggtggagcc cgatcgcccg
     2641 gacgccgggg gcgccgccgt tcgactcgcg gaggtcggcg gcgattgctt gcgccaggtc
     2701 gaggtcgtct gttgccaggt cgacgttgaa ggcgatcaga ggcgggcgag cggtgacgag
     2761 cacggcgccg gcggcggggt cgacgtgcgc ggggccgtag tcgggggcga ggtcgcccga
     2821 cgccacgcgc tcggcgaggg tggttgtgcc gcccgcccgg aggttcgctc gctcgcgatt
     2881 gccgggggcg gtggcgaggt cgccgtagag gaagacgggc aggccggcgt cgccgatcag
     2941 cgccgcggcg gtaagcgcct cggcgcaggc ggcgccgcgc tgctcgtggt cgaggtagac
     3001 caccggtgcg acgtcgagtg cgccgacgtg cgggtggcgt cccgtgtgct cgcgcaggtc
     3061 gatccgctcg cgcgcggccc gggcgccagc cgcgagcgcg gcggccagct ctccctgttc
     3121 gcccgcgagg ctgaacacgc tgcgaccgtg gtcggggtcc gagtgcacgt cgagcacgcg
     3181 cgcgggcgcg aacgcgtcgc cgatcgcggc gatagcggcg cgatccgatc cctcgctgac
     3241 gttgggaacc gcgagaagct gcatctagac tggggcgcgc tcggctccgt agctcagtcg
     3301 gttagagcgc agcactcata atgctgaggt cgctggttcg agtccagccg gagctatcag
     3361 ttgttgcgac acacccggcc ttatcgcccc agctgtgccc aagaagccga ccgggacgat
     3421 cgtcgtgtac cacccggagg ccgtcgacga cttccagggc atcaaggaca agcaggcgcg
     3481 caagaaagtc ctgacgatcg tctcgatcct tcggcaggcc ggcacgagca tcaccgagcc
     3541 gcatgtgaag aaggtgcaag gcgaggcgaa gttgtacgag ctgcgtccgg gcggtggcaa
     3601 gacactgctg cggccgctgt atgcccagat cgacgcctac ctggtgatcc tggccatcgg
     3661 gcctaaagcg gaggtcaacg cgtccggttt tgctgcggcc gtcaagcggg cgaagcgtcg
     3721 agcgaaggaa gagttcggcg tcgagattta gtttctgtgt gagggtccga taacatgcgt
     3781 gctatcgcca tcgcggatcg acgactggag caggtatggc ccgtaagaag ctgaccaaga
     3841 tgaagaccca cggcgagctc gtggccgatc agcagctggt cgatcccgat ttcaaccgcg
     3901 aatgggagcg gctcgcaatc tcgcgtgccg tcgccgcccg cgtaatcgcc taccggggcg
     3961 acaacaagct gagccagcgc gacctcgcca agctcttgga gatgccccag ccacaggtca
     4021 cgcgcttgga gagcggcgag taccacccct cgaacgagac cctctcccga ctcgcaagca
     4081 agctggggat ggagttcacg atcagcatcg cacccgcgaa gagcgaaccg aagcagatca
     4141 cgaagaggac gcgcgagcat gccgacgcca tggtcgaagc gggcgacagc atcacgcgct
     4201 tcagcgtcgc ctaatcccac gcggcctcat cccgcggccc tccgccaccg ggtgagcacc
     4261 gcggcgccgg agcgctcggc cgccaggcat tccatgtcca ggggcggact gccggcaaag
     4321 agccgctcgc cggcaccgag cacgacgggg aacgtcagca gccggtactc gtcgatcaga
     4381 tcctcggcca tcaacgcgtg cacgacgctg aggctgccgg tgatgatcac gtcacgccgc
     4441 tcgcgcctga ccacgtcgag cagctcgccg tcgatgggtt gcgagttcgc ccacgccgag
     4501 gcgtcggtca gggtgcgggt gacgaccagc ttggggacgg cgttcatccg cgtggagaac
     4561 gggtcgtcgc gtcccggcca gatcccggag aacaactgcc aggtcttgcg tcccagcagc
     4621 atgaccccgt cgtccagcac gctgtcgagc cggaacttgt cccccgcgat cgcctcggga
     4681 ccgaaccgga acgcccagcc gccgcctggc gtgcccaggg acccgtccgg gtcggacacg
     4741 atcccatcca cggtgatgaa ctcgatgacg attacgctca cgatcacggc ccccttgcgt
     4801 cggtttggcc ggtacatacc ggcgccaccg cccggactca tcgcggtcag cgaagcgcgt
     4861 cagacgagat tcgctcaggt aggccaaacg ccgccagcac ggacggatgg gcgaagacga
     4921 cgttgcgcgc aacgcggccg ccggtgaccg tgaggacctg gagggtgtgg agcgcatgcc
     4981 cgccaccggg ctcgggcgcg taggccgcga gtgcgggctg cccgttcgcg gtgagctgcc
     5041 tcatcgccca gccgagaccc cgcatgtcga acacgcggcg catgaagagc ccgtactcgc
     5101 ggctgcctcg gtaccacagt ggcaccggcg gcatctcgag caccgcgtcg tcggcgagca
     5161 gccgcaccag gccgggcacg tccgcggcct cgaatgcgtc cacgtaccga tcgacgatcg
     5221 ctcgcgcccg cggatcgtcc ggttcggtga cctcgtccac atcacgtgtc tccgcaaggg
     5281 ccccacgcgc tcgctgcaga gcgctgttga cggccgccac cgtggtgtcg agttgcgccg
     5341 ctacctctga agcgctgaac ccgagcacct cgcggaggat cagcaccgcg cgctgtcgcg
     5401 gcggcagggt ctggagcgcg gccacgagcg ccaaccgcac atcaacccgc acgcccgcgt
     5461 cgaaccgcgc atcgggaaac ggctgcagcc aggggacatc caacgcgggc gtcagcggcg
     5521 cgccgggatc gtcgctcggt gcgaacagcc cggacggcaa cggccgccgc gcccggccct
     5581 ccaacgccga caggcacgcg ttagtcgcga tccgatagag ccaggtccgc accgacgccc
     5641 gggcgccgtc gtagcgatcg cgcgcccgcc acgcccgcag cagggtctcc tgcaccaggt
     5701 cctcggcctc gtcgaaggac cccagcatcc ggtagcagta cgccaccagc tcacctcgat
     5761 agggatcgaa atccaccagg ccctcctcgc gcacacgtcc ctcccgttgg atcatcgctc
     5821 cgtttagtac cggagggccg cacgaggctc atcgggcatc gggtccggtt gcgacgcggg
     5881 agcgcgccag ctgcgcttga caaatggcgc gcttgcacac acggttgaga ttcggaacct
     5941 ttctaggtag cagggggact cgcatgactc gcacgcgttc gcgcgcgctg gccctggtgg
     6001 ccgcgctcgc gctgattgtt gctgcaccgg ccggcgcgga cagctcggca acggagacgg
     6061 ccgcgatcac ggcggcgaag agctgctcga gcggctggaa gcacgccgtc ctgccgaacg
     6121 ggcacaagtg cctccgcgcg ggccagtact gcaagaagtc gtgggacagc cgctaccacc
     6181 actacggctt ccactgccac aacggccgcc tcaagaggaa gtagcgctac gcgacgctga
     6241 agctgctcgt cagctcgggc gaggcggcca acgtctggta gacgacgcag tagcgctcgg
     6301 tgagcttgcg gagcgtcgcg agctgcgcct cgtcggcctc ggtgtcgagc tcgaaggcga
     6361 gccggatcga cgcgaacccc accggagcct cgcggtccac cgccagcgtg ccgcggaagt
     6421 cgaggtcgcc ctcggcccgc acggtgccgg acgcgacctc gatgccgagc gaggtcgcga
     6481 ccgcctggag cgtcacgccc gcgcacgcga cgagcgcctc gaggagcatg tcgcccgagc
     6541 agaggagcga gccgtcgccg ccggtcgccg ggtgaaggcc ggcctccgcg agcgcccgcc
     6601 cgctctgcac cgtgcacgac acgttctcgc cgagcgtgcc cgacgccgac agcgtcacga
     6661 gcgccgcctc cggctcgctc gcgtaccgct ccttcagcgg ggcctgcacc gccctgagct
     6721 cgtcgcgctc cacagaggcg gatgctaggg accggcggcg tcggtaggtt gcgcatccga
     6781 cccgatccct cgtccaggag accgccttga agagactcgc cctcgccgcc gccgcggcag
     6841 ccgccctcgc cctgcccgcg ccggccggag cgatgatcca gttcgaccag ggcatcgccg
     6901 gtgcccggat cggcaactcg caggcggagg ttcaggccgc gctcggctcg ccctcgctcg
     6961 tcaagaccgg cacgaacgag ttcggcccgt ggcgccagtt ctacttcgcg ggcggtctgc
     7021 gcgtgttctt ccagggcgac gaggacgtga cctccgtgac gctgctcggt cgcggcgacc
     7081 gaaccaccaa gggcatcggc gtgggcaaca ccgagaaggc gctcaagcag aagcacccca
     7141 gcctcacgtg caagaccaac agcggcttcc gctcctgcta caccggcgac ttcaagcccg
     7201 gcgagcgggt gacggacttc tggatctcga aggggaggat cacccgcgtg gtcgtcggca
     7261 tcgtcatcga ctaggaggat cgtccactgg agcgttgaag aggcccggca tgcagcctcg
     7321 cgatcgactc atgccggccc tcacgacgct cctgctgatc gtggccctgt tcgccacgat
     7381 cggcatctgg aagcaggccg gggcgacctc cgacgaggcc gatgcgaccg agaagcgcgc
     7441 ctgcatcgag tcggctgagg cgcgctaccc ggcggtgtcg gtgtcggcgt tcgtcacgcg
     7501 cgatcgctcc gcctcgggcc cgctcaagct gtcgttcgtt cgcgagcgcc agcgggccgt
     7561 cgccgcctgc gattgatccc gtaacccgtc gggccgacca gttcccccat atgggggtca
     7621 aggtcgcgtc tgccggaccg ataaaggcag ccgcccgccc gaccgataca gggctggatg
     7681 cttccttgga cacgctccaa gtggttccgc ctagccgtta ccgggttctc gacgatcttc
     7741 gtcgcgatcg tgctcgccac gtcgctgatg gccgagggca gcgccctcaa gcgagcggac
     7801 gtggagtacg acgccgtcga ctgcgtcaag gacaagaaca cgaagggacg cgcgcggccg
     7861 gccaccggct gcaagcgcaa gaagcgagtc gtcatcccga tcgacgacaa gcagcagatc
     7921 tcggaggagg agctggagcg cgcgctcgac gagctgccgc gcctcagcgg cccgcgtggc
     7981 ctcaccggcc atcgcggcgc gaacggacgg cgaggcccga ccggtccgcg cggcacggct
     8041 ggtgtcggac gcgcaggccg gcgcggagcc accggggcgc gcggcaggtc ggcgcagggt
     8101 gttcgcgggg ctgagggcgc gcgcggggcc gacggcgagc gcggcgccag gggcgacgac
     8161 ggtgcgcgcg gcgccgaggg cgaggccggt ccggccggtc cgaccggcga gaccgggccc
     8221 gcgggcccgc agggacttca gggcgacacc ggcgcgcagg ggccgcaggg cgacaccggc
     8281 gccaccggcg cgcagggcga tgtcgggccg cagggcgtcc agggcgatgt cgggccgcag
     8341 ggaattcagg gcgacaccgg cgcacagggg ccgcagggcg acacaggtgc gcagggcccg
     8401 accggccccg ccgggccgca gggcgatgtc gggcccaccg gaccgcaggg cccgaccggc
     8461 cccgccggac cgcagggcga tgtcggcccg cagggcgcca ccggcgccac cggtccggcc
     8521 ggcccgcagg gaccgcaggg agacatcggg cccgcgggcg cgcagggtcc gcagggcgtg
     8581 accggcgcaa cgggcgcgac cggcgcgacc ggtgcccagg gcccgcaggg cgacatcggc
     8641 cccacggggc ccgcgggtcc gcagggcaac accggacccg ccggacccgc aggcccgcag
     8701 ggcaccacgg gcgcaactgg tgccaccggt gccaccggcg ccacgggtgc gcagggaccg
     8761 cagggtcctc agggccccca gggaccgcag ggtccgaccg gcgccacagg cgccaccggc
     8821 gcgaccggtc cgcccgcggc gaagtactgg gcagtcgtcg acagcaccgg ctcgctcgcg
     8881 cgctcgagcg ccggcgtcaa ctcctcgcgc atcacgaccg gccagtaccg cgtcgacttc
     8941 ggaaccgacg tgacccagtg cgcgtacatc ccccagatcg gcgggaccgg gaccaccgcc
     9001 ccgagcgtcg ggatgatcca ctc
//
LOCUS       DASVPL010000313         6088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_153333, whole genome shotgun sequence.
ACCESSION   DASVPL010000313 DASVPL010000000
VERSION     DASVPL010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153333"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VF517_17965"
     CDS             complement(<1..899)
                     /locus_tag="VF517_17965"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX8054876.1"
                     /translation="MSQRPAPSGSPRGGFPLTDEQRAAVAGRDEPRFVRAGAGSGKTR
                     VLVERFLAAVREDGVPVDRILAITFTEKAAAELRSRVRERLLAAGDREGARQAESAAI
                     STIHGFCSRLLRSHALDAGLDPDFRVLDQVEAARIALDAFDFALERFLESAADEERLD
                     LAAAYSPDRLRTMVVTVHGRLRSRGEREPRLPPVPAPEPGGERERLADAAARAGRELN
                     GESKSIDAARAALERCGTFFVRLPEGAIPDPEDLKPLEVKRGNAKALKTPAFDELAEA
                     YEAYVNFCAASRAAAHNVLLSELL"
     gene            complement(896..3820)
                     /locus_tag="VF517_17970"
     CDS             complement(896..3820)
                     /locus_tag="VF517_17970"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX8054877.1"
                     /translation="MPLKLVTGPANAAKAGVVFGDYRAALDREPILVVPAFRDVAHSQ
                     RELAERGAVFGTRVVRFRWLFEIVAERCGSESLGARKAAPLQRELIVERAVRDARLEV
                     LVDSARRPGFVRAAERLIRELERSMVTPARFGQALAAWAGDGARRPYADEVAAIYRSY
                     RATLERAGLVDDELYAWHALDALRARAADWGGGAVFVYGFDDFTPIERGLIEALADDP
                     DTQVVVSLPYEEGRAAFEAVEPLVRDLAERASEHEVLPASAEHYAPESREALNRVERL
                     LFAPDGGEADPAGAVRLLSSGGERAEVELVAAEVLRLLRAGTPAGQVAVVFREVAPYA
                     SLIEQVLSAYGIPFALERWVPLGHTGLGRGALALLRCAGPEGTADDLLTYLRTAGRLD
                     QPHLADRLEHEVRRLGARTAAAAREIWERDRWQLGEIDRIARARDAATLAQQLDEEVE
                     RLFARPYERTAHVFADEEGEDPRAREALRGALSGIQRLARADAGLAPPRERLHDLLTT
                     VEVRTGAAPAPDRVQIARPQDIRARRFDAVFVCGLQEGEFPKPPRPEPFLSDEDRRAI
                     AQATGLALPLREERPARERQLFYVCASRAERCLALSWRETDEEGSPQLRSFLVDDVRA
                     LFGERLREPDATRPLSSVTWAAEQAPTEVEWERAAALAGPRRSPPRPERIEAEDVLAD
                     LASTTRLSPAALESYADCPVKWLVERRLRPDALEPDPEPMVRGRYAHAVLELTYRRLG
                     ERTGSRRVTHKSLAEAERILLGALRERQSEFRLSPQATRVRAAVRRLEVDLLRYLRAE
                     ADAGSVFEPAELELDFGLEGSLQPPLVLDDGLEVGGRIDRVDVWDGHAIVRDYKGGSK
                     VEGVAKWGERNRLQVALYMLAVREVLGLRPAGGVYQPLKGRDRRPRGVLLKGLEDELG
                     TGYVGNDFRDEEEIERELDAARERLLELAARMRSGEVRPCPETCAWNGGCSYPSICRI
                     ER"
     gene            complement(3925..4329)
                     /locus_tag="VF517_17975"
     CDS             complement(3925..4329)
                     /locus_tag="VF517_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054878.1"
                     /translation="MSADGVRHARMEDVPAERYAAGLDWRPVRHHLGIEAFGAGTWHG
                     DTGGEVIEEHTETEGNADGHEELYYVISGRATFTVAGEEIDAPAGTFVAVADPELERR
                     AVAQEDGTAILAIGAPRGRVYEVSPWERRRLG"
     gene            complement(4326..4637)
                     /locus_tag="VF517_17980"
     CDS             complement(4326..4637)
                     /locus_tag="VF517_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054879.1"
                     /translation="MQAESHGISIRLAAPADGEAVRQLALLDSAERAEGETLVAEVDG
                     RIRAALPLAHGRVIADPFVPSAELVSLLELRARQLRAETVEERPLRALAHALPVVGRL
                     A"
     gene            4732..5313
                     /locus_tag="VF517_17985"
     CDS             4732..5313
                     /locus_tag="VF517_17985"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX8054880.1"
                     /translation="MAAGTKPVDNNSSRELAPGELATLQQFLNTRDLEDGIELLQAPA
                     DLRRWLTHHELVAPSTRIGSAELRRALDVREAFRAVLWAKDGGPVDRAAIETLDAAAR
                     RAELTVRWHESGEAGLAPARSGLDGAFGRLLAIAYRARVEGTWERLKVCPDDSCGWAF
                     YDRSRNRSSTWCSMAVCGNRAKARAFRERRARR"
     gene            complement(5392..6024)
                     /locus_tag="VF517_17990"
     CDS             complement(5392..6024)
                     /locus_tag="VF517_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054881.1"
                     /translation="MTSLFCRHNRFTADCSICSKGTVLDSARAGERPRAKSASGSRAA
                     RGSGGRGAQGGGTRTFSGPHVQAGPYEREEDTYHVRLERVPGGLRLAQWSRGSLERRA
                     PELPEGDLAGLVVAALGKELIEGRDAERLRDALGQNGNEPADGASPGRAGDMREELRV
                     ERLSTGRVRVGRWLFWPGRGWELQEAPPMFPAARYAEALAAAGVAAQGDA"
BASE COUNT          757 a         2385 c         2221 g          725 t
ORIGIN      
        1 agcagctcgg agaggagcac gttgtgggcg gccgcgcggc tggcggcgca gaagttcacg
       61 tacgcctcgt acgcctccgc cagctcgtcg aaggccggcg tcttcagggc cttggcgttg
      121 ccacgcttga cctcgagtgg cttgaggtcc tcggggtcgg ggatcgcgcc ctccgggagg
      181 cggacgaaga aggtgccgca gcgctccagc gctgcgcggg cggcgtcgat ggacttgctc
      241 tcgccgttca gctcccgccc cgcccgggca gccgcatcgg ccagccgctc gcgctcgccg
      301 cccggctcgg gggcggggac cggcggcagc cgcggctcgc gctcgccccg gctgcgcagg
      361 cgaccgtgca ccgtcaccac catcgtccgc aggcggtccg gcgagtaggc cgcggcgagg
      421 tcgagccgct cctcgtcggc cgccgactcc aggaaccgct cgagggcgaa gtcgaacgcg
      481 tcgagcgcga tgcgcgccgc ctcgacctgg tcgagcacgc ggaagtcggg atcaaggccc
      541 gcgtccaggg cgtgcgagcg cagcagtcgc gaacagaagc cgtgaatcgt cgagatcgcc
      601 gccgactcgg cctgccgtgc gccctcgcgg tcgcccgccg ccaggagccg ctcgcgcacg
      661 cgcgagcgca gctccgccgc ggccttctcg gtgaaggtga tcgcgaggat ccggtccacc
      721 ggcacgccgt cctcgcgcac ggccgccagg aagcgctcga ccagcacgcg cgtcttgccg
      781 ctgcccgcgc ccgcgcgcac gaagcgcggc tcgtcgcgcc ccgcgacggc cgcgcgctgc
      841 tcgtccgtga gcgggaagcc gccgcgcggc gatccggagg gtgcggggcg ctggctcacc
      901 gctcgatcct gcagatcgac gggtacgagc agccgccgtt ccaggcgcac gtctcgggac
      961 acggccgcac ctcgcccgag cgcatccgcg ccgccagctc caggaggcgc tcgcgcgcgg
     1021 cgtcgagctc gcgctcgatc tcctcctcgt cgcggaagtc gttgcccacg tagcccgtgc
     1081 cgagctcgtc ctcgagcccc ttcagcagca ccccgcgcgg gcggcggtcg cgccccttca
     1141 gcggctggta gacgccgccg gccggccgca ggccgagcac ctcgcgcacg gccagcatgt
     1201 agagcgccac ctggaggcgg ttgcgctcgc cccacttcgc gaccccctcg accttcgacc
     1261 cgcccttgta gtcgcggacg atcgcgtggc cgtcccagac gtccacgcgg tcgatcctcc
     1321 cccccacctc gaggccgtca tccagcacaa gggggggttg cagcgatccc tcgagtccga
     1381 agtcgagctc cagctcggcc ggctcgaaca cgctccccgc gtcggcctcc gcacgcaggt
     1441 agcggagcag gtccacctcg agccgccgca cggccgcgcg cacccgtgtg gcttgcggcg
     1501 agagccggaa ctcggactgg cgctcgcgca gcgcgccgag caggatgcgc tcggcctcgg
     1561 ccaggctctt gtgcgtgacc cgccgcgagc cggtgcgctc gccgagccgg cggtaggtca
     1621 gctcgagcac cgcgtgcgcg tagcggccgc ggaccatcgg ctcggggtcg ggctcgagcg
     1681 cgtccgggcg cagccgccgc tcgacgagcc acttgaccgg gcagtccgcg taggactcga
     1741 gcgcggccgg ggagagcctc gtcgtcgagg cgaggtccgc cagcacgtcc tcggcctcga
     1801 tccgctccgg ccgcggcggc gagcgccgcg ggccggccag ggcggcggcg cgctcccact
     1861 cgacctcggt gggcgcctgc tccgccgccc aggtgaccga cgacagcggg cgcgtggcgt
     1921 ccggctcgcg cagacgctct ccgaacagcg cgcgcacgtc gtccacgagg aacgagcgga
     1981 gctggggcga gccctcctcg tcggtctcgc gccagctcag cgccaggcag cgctcggcgc
     2041 gcgaggcgca gacgtagaag agctggcgct cgcgcgcggg ccgctcctcg cgcagcggca
     2101 gcgcgaggcc ggtggcctgc gcgatcgcgc ggcggtcctc gtcggagagg aacggctcgg
     2161 gccgcggcgg cttggggaac tcgccctcct ggagcccgca gacgaacacg gcgtcgaagc
     2221 gccgggcgcg gatgtcctgg gggcgcgcga tctgtacacg gtcgggcgcc ggggcggcgc
     2281 ccgtgcgcac ctcgacggtc gtaagcaggt cgtgcaggcg ctcacgtggc ggggccagcc
     2341 cggcgtcggc gcgcgctagc cgctggatgc ccgacagggc gccgcgcagc gcctcgcgcg
     2401 ccctcgggtc ctcgccctcc tcgtcggcga acacgtgagc ggtgcgctcg tagggccgcg
     2461 cgaacagccg ctcgacctcc tcgtcgagct gctgcgcgag cgtggcggcg tcgcgcgccc
     2521 gcgcgatccg atcgatctcg ccgagctgcc agcggtcgcg ctcccagatc tcccgtgccg
     2581 cggccgccgt gcgcgcgccc agccggcgga cctcgtgctc gagccggtcg gcgaggtgcg
     2641 gctggtcgag ccggccggcc gtcctcaggt aggtcagcag gtcgtcggcg gtgccctcgg
     2701 gacccgcgca gcgcagcagg gccagcgcgc cgcggccgag gcccgtgtgg ccgagcggca
     2761 cccagcgctc gagcgcgaac gggatcccgt aggcggagag cacctgctcg atcagcgacg
     2821 cgtagggcgc gacctcgcgg aacaccaccg ccacctggcc cgccggggtg ccggcgcgca
     2881 gcagccgcag cacctccgcc gcgacgagct ccacctcggc gcgctcgccg ccggacgaga
     2941 gcagccgcac cgcacccgcc gggtccgcct cgccgccgtc gggcgcgaac agcagacgct
     3001 cgacgcggtt gagcgcctcg cgcgactcgg gtgcgtagtg ctccgccgag gccggcagca
     3061 cctcgtgctc gctcgcgcgc tctgccaggt cgcggacgag cggctcgacc gcctcgaacg
     3121 ccgcacgccc ctcctcgtag gggagcgaga cgacgacctg ggtgtccggg tcgtcggcga
     3181 gggcctcgat cagcccgcgc tcgatcggcg tgaagtcgtc gaacccgtag acgaacacgg
     3241 cgccgccgcc ccagtccgcg gcccgggcgc gcagggcgtc gagtgcgtgc caggcgtaga
     3301 gctcgtcgtc cacgagcccg gcgcgctcca gagtggcgcg gtaggagcgg tagatcgccg
     3361 cgacctcgtc ggcgtagggg cggcgcgcgc catcgcccgc ccaggcggcc agcgcctgac
     3421 cgaagcgcgc cggcgtgacc atcgagcgct cgagctcgcg gatgaggcgc tccgccgcgc
     3481 gcacgaagcc cggccgccgg gccgagtcca ccagcacctc gagccgcgcg tcgcgcacgg
     3541 cccgctccac gatcagctcg cgctgcagcg gggcggcctt gcgcgcgccg agcgactccg
     3601 acccgcagcg ctcggccacg atctcgaaca gccacctgaa gcgcaccacg cgcgtgccga
     3661 agaccgcgcc gcgctcggcc agctcgcgct gggagtgggc gacgtcgcgg aacgccggga
     3721 ccacgaggat cggctcgcga tcgagggccg cgcggtagtc gccgaagacg acgcccgcct
     3781 tggccgcgtt ggcgggcccg gtgaccagct tgaggggcac ccggccattc tggggccggg
     3841 atccgacgcc gcgggaggct cgctccggct ttgcccgcta tgtgggcaga tccgcgcgct
     3901 tcgaggcgcg gggtggcgcc gggctcagcc caggcggcgg cgctcccacg gcgagacctc
     3961 gtacacgcgc ccgcgcgggg cgccgatcgc gaggatcgcg gtgccgtcct cctgagccac
     4021 cgcccggcgc tcgagctccg ggtccgcgac cgcgacgaac gtgcccgccg gggcgtcgat
     4081 ctcctcgccg gcgaccgtga acgtggcccg gccggagatc acgtagtaga gctcctcgtg
     4141 cccgtcggcg ttcccctccg tctcggtgtg ctcctcgatc acctcgccgc cggtgtcgcc
     4201 gtgccaggta ccggcgccga acgcctcgat gccgaggtgg tggcgaaccg gccgccagtc
     4261 gaggccggcc gcgtagcgct cggccggcac gtcctccatc cgcgcgtggc gcacgccgtc
     4321 cgcgctcacg cgagcctccc gaccaccggc agcgcgtggg ccagcgcccg cagcggccgc
     4381 tcctccacgg tctccgcgcg gagctgtcgc gcacgcagct cgagcagcga gaccagctcg
     4441 gcgctcggga cgaacgggtc ggcgatcacg cgcccgtgcg cgagcggcag cgcggcccgg
     4501 atccgcccgt ccacctccgc gaccagcgtc tcgccctccg cgcgctcggc cgagtcgagc
     4561 agcgcgagct gccgcaccgc ttcgccgtca gccggcgccg ccagcctgat cgagatcccg
     4621 tgtgactccg cctgcatgac cgacctcccc gaaacccgtg taaccgtcat aagaactata
     4681 ccagttacgt acctagtgaa acgagttgac tggttaccct aagcttcagc catggcggca
     4741 gggacgaagc cagtggacaa caactcgagc cgcgagctcg cccccggtga gctcgccacg
     4801 ctccagcagt tcctcaacac gcgggacctc gaggacggca tcgagctgct ccaggcgccg
     4861 gcggacctgc gccgctggct cacccaccac gagctggtcg cgccgagcac gcgaatcggc
     4921 tccgccgagc tgcggcgcgc gctcgacgtc cgcgaggcgt tccgcgcggt gctctgggcc
     4981 aaggacggcg ggcccgtgga ccgcgccgcg atcgagaccc tcgacgccgc cgcccgccgc
     5041 gccgagctga cggtgcgctg gcacgagagc ggcgaggccg ggctggcccc cgcccgcagc
     5101 gggctcgacg gggcgttcgg gaggctgctg gcgatcgcgt accgggcgcg tgtggaggga
     5161 acctgggagc gcctgaaggt ctgtcccgac gacagctgcg gctgggcctt ctacgaccgc
     5221 tcgcgcaacc gttcgagcac gtggtgctcg atggcggtgt gcggcaaccg cgcgaaggcg
     5281 cgcgcgttcc gcgagcggcg ggcgcggcgc taggggcgca cgtccggcgc taggggggcg
     5341 cgtccgggtc aagcgtcgcc aggcgcggcg ctagtggcgg cgcgtccggc gctacgcgtc
     5401 gccctgcgcg gccacgccgg ccgccgccag cgcctccgcg tagcgcgccg ccgggaacat
     5461 cgggggcgcc tcctggagct cccagccgcg gcccggccag aagagccagc ggccgacccg
     5521 cacgcgaccc gtggagagcc gctcgacgcg cagctcctcg cgcatgtcgc cggcgcggcc
     5581 gggcgaggcg ccgtccgccg gctcgttccc gttctgcccg agcgcgtcgc gcagccgctc
     5641 ggcgtcgcgg ccctcgatca gctccttccc cagcgcggcc accaccagcc cggcgaggtc
     5701 gccctccggc agctcgggcg cccgccgctc gagcgacccg cgcgaccact gggcgagcct
     5761 cagcccgccg ggcacccgct cgaggcgcac gtggtaggtg tcctcctcgc gttcgtacgg
     5821 ccccgcctgc acgtgcggcc cgctgaaggt ccgcgtaccc ccgccctggg ctccccgccc
     5881 accggacccg cgcgccgcgc gcgacccgct cgcgctcttc gcccgcggtc gctcgccagc
     5941 gcgcgcggaa tcgagcaccg tgcccttcga gcagatcgag cagtcggcgg tgaagcggtt
     6001 gtggcggcag aagagcgatg tcatttagcg ggcgaccaga gtaccgctgc tttcatcccg
     6061 gcccagcatg cgcaccgccg tcatctcg
//
LOCUS       DASVPL010000314        10410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_311768, whole genome shotgun sequence.
ACCESSION   DASVPL010000314 DASVPL010000000
VERSION     DASVPL010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311768"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VF517_17995"
     CDS             <1..560
                     /locus_tag="VF517_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054882.1"
                     /translation="LFHVAKLETTAKLTDAVLKGQDIVLLLKLARDDGDWTMRSLGEA
                     LDLDPASVHRALRRLDEARLIDIKRRRVNRGRAEEFLLHGFQYVFPVHQKGTSRGLPT
                     AWAAPPLSDELALPDDPPPVWPDAKGKTRGVAVEPIHASVPALARRDPEMGERLALLD
                     GIRLGDARVRKLASQHLVRRLDAGN"
     gene            557..1243
                     /locus_tag="VF517_18000"
     CDS             557..1243
                     /locus_tag="VF517_18000"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX8054883.1"
                     /translation="MSIGLLERAGADLGALVDEVVFVGGATLALWITDPGAPPIRPTK
                     DVDVIVEVTTRGGYYEFEERLRAASFAPEGSVICRWIQRDTALIVDAMPADPAILGFS
                     NRWQRAALPFAAEVQLLSGARIRAVPPPFLLATKVEAFGGRGQDDFLGSRDFADIVSL
                     VDGREQIVDEVRTAPPKVRSYLADELSSLMSRARFAEGVSAQLPPDEASQERADLVVA
                     PRLRAIIDAA"
     gene            complement(1403..1960)
                     /locus_tag="VF517_18005"
     CDS             complement(1403..1960)
                     /locus_tag="VF517_18005"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8054884.1"
                     /translation="MRNDIVSRAELATGRLDGTEEVAVCFADLVGFTRLGEEIPPEDL
                     SRVAGRLTDLAGDVARPPVRLVKTIGDAAMLVGRETDAVVEVALELVERAAEAGDEFP
                     ALRSGIARGPALERAGDWYGSPVNTASRVTGVARPGSVLCTADAREAAGDGFRWSPAG
                     RRKLKGVGEPVRLFRARRETDKPAE"
     gene            complement(1976..2503)
                     /locus_tag="VF517_18010"
                     /pseudo
     CDS             complement(1976..2503)
                     /locus_tag="VF517_18010"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
     gene            complement(2524..3414)
                     /locus_tag="VF517_18015"
     CDS             complement(2524..3414)
                     /locus_tag="VF517_18015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8054885.1"
                     /translation="MEREEITLHGHRVSYRQAGWGPVIVLIHGITGSSDTWLDVIDEL
                     AEDHTVVAPDLLGHGESAKPRGDYSLGAYASGIRDLLAALGHDRATVVGHSLGGGVAM
                     QMAYQFPERVERLVLVSSGGLGREVNILLRAAALPGSEFVLPLLTARPIVEWTNSIAG
                     AVGGLGLRAGADLEEIWRGFSSLCDVEARAAFIHTLRGIIDPGGQRVSATDRLYLAAE
                     MPSLIVWGERDPVIPFAHGLRASDSMPGSRLAAFPNAGHFPHRDDPRRFTHELLDFMA
                     ETDPSVLPEERLRELLREGA"
     gene            complement(3448..5247)
                     /locus_tag="VF517_18020"
     CDS             complement(3448..5247)
                     /locus_tag="VF517_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054886.1"
                     /translation="MTQIIPLAAATEPAGGAATGEAIGATVGALVLTALMLAVLVPHR
                     NGRDGVLARAADFTARQTGLPGWAALPSMISGVSLVVALLGMYWDISLHIDNGRDPGP
                     LANPAHYLILAGLFGVLFAGLVAVCLPREKPSDVAVRIAPGWHAPLGGVLMVGCGVFS
                     LAGFPLDDFWHRLFGQDVTLWGPTHLMLIGGAALATISAWVLFVEGTARRGPRVRRLP
                     LLKLRQAAVAGGLLVGLSTFQAEFDFGVPQFRLLFHPILLMLAAGIALVVARVRIGRG
                     GALIAALSYLAVRGLVSLLVGPVFGQTTPHFPLYLGAALAVELVALRVSTDRAVRFGA
                     LAGVGIGTLGLAAEWGWSHVWMPIEWPTALLPEAALLGFVSAVAAGALGGAMGRALSN
                     DIANPVTRPERFALAAAAVAAVAALGYTLPVSEGTPTTARVTLDEVRAAPNREVVATA
                     TLDPPDAADGAEWLNVTAWQGGGSRIVRMREVGPGTYRSAGPIPVDGSWKAILRLHSG
                     NRIVGVPIFMPRDTAIPAAEVPASPRFERAFALDRKNLQREQKDGVPGWLTLAGYLTV
                     LAIASAMIAALTVALARLGGARARSGRGAATPA"
     gene            5295..6056
                     /locus_tag="VF517_18025"
     CDS             5295..6056
                     /locus_tag="VF517_18025"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8054887.1"
                     /translation="MSSSTEDTRAAKAARGTRSGAKPARGGASAKAPPAGGDKRGGSA
                     PPRRRLSAEQRRAAILDAALEVFAKHGYHAASIDDIAGVATVSKALIYEHFPSKRHLH
                     AALLERHVGELFGLLVESAATSEPGEVRLRAGVDAFLAFVETRPDAFRMLFRDAVEPE
                     VAEVLRTLGQQAARQVAELIATEPRERRADDEEEDEAIEMLGALLTGAVQSLALWWDE
                     HPKVPRERLVERTMEFAWLGFERLRAGERLKETAA"
     gene            6053..7801
                     /locus_tag="VF517_18030"
     CDS             6053..7801
                     /locus_tag="VF517_18030"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8054888.1"
                     /translation="MRFGLFYEHQEPRPWEEGSTERLLNDALDQVELADRVGFDYVWE
                     VEHHFLEEYSHSSAPEVFLAAASQRTERIRLGHGIVQLPPAFNHPVRVVERAATLDLI
                     SGGRVDLGTGEASSQVELGGFGVDRNAKRAQWEESIDAVARMFVEEPFAGLDGEHVSI
                     PPRNVMPKPKQKPHPPLWVACSRRETIIMAARRGIGALSFAFIEPEAAKEWVDEYYAL
                     IESDECVPAGFDVNADFAVVLPMMLHEDEATAIERGIDGAHFFGYSLAHYYVFGEHVP
                     GRTNIWEEFQRDRSEKGFAREIVSPDDEPLGVKLLQQGMGSLRGAVGTPGQVEELCRR
                     YEAAGVDQVIFVLQAGRNRHEHVCESIELFGERVLPGFAERAGAREAEKAERLAGAVE
                     RANARREPPRRAPDGYVITPQSEPAPADAMRAAKQPARPADAVTGGGGVAQRLGRIGE
                     AAFAAMVRGRSEEQLERALGNDVALRLVFGGMERAFVPERAEGFEGAIQYELESQRGT
                     RRWHVEIADGRAVARPGATSDPEVTLRAQVPVFARIFARELNPAQALLAGDLAVEGDF
                     MVAGRIGYMFGEDSPF"
     gene            7846..8208
                     /locus_tag="VF517_18035"
     CDS             7846..8208
                     /locus_tag="VF517_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054889.1"
                     /translation="MRGIARDVQHRTEIYGTGTDGNVSSRHVLTFRVERPGSTPVPVQ
                     MKGMRIEGVLADGDEVSVEGEQRPGKVMKTKRVENLTTGVPVVAGVPLYVKIVAIPVV
                     AAVIAMFSYVAYTFATGP"
     gene            complement(8205..9374)
                     /locus_tag="VF517_18040"
     CDS             complement(8205..9374)
                     /locus_tag="VF517_18040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8054890.1"
                     /translation="MTTPPALQLVPSDEEARIRETVAAICTGYGPAYSRQKRLAGEPP
                     TELWDDLASRGYLGVNIPEEYDGGGLGMSALAAVGEEISAAGVGLLLIVVSPAIVGSI
                     LVRHGTPDQKERWLRGIGRGTTKVAFAITEPDAGSNSHNISTAAVRTDGGYALKGQKT
                     FISGVEDADAILVVARMREPDGSLGLPVIALVDSDAPGLERQEVVTVASGPDKQWLLF
                     FDDVEIERDRLVGGETAGLKAVFDGLNPERIMGAAICNGASRRALALAADYARERQVW
                     KTPIGAHQGISHPLAEAKIELELARLMMQKAAALYDAGAPAGEASNMAKFAAAEAGIH
                     CVDRAIQTHGGNAFTHEYGLPDLWWAVRIARTAPVSREMVLNYVAEHSLGLPRSY"
     gene            complement(9371..>10410)
                     /locus_tag="VF517_18045"
     CDS             complement(9371..>10410)
                     /locus_tag="VF517_18045"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX8054891.1"
                     /translation="RPALGVFSHEYACVSESDRRVYLTEDQSGGGFYRFTPRRWGDLS
                     EGVLEIATLVANGVVEWRVIPDPLAQSTPTRQQVASSRMARPEGLCVHRGTGLVYFVE
                     SSAGRVFQYDPRTETYERIYSESDFPEPELTDTDNIAVSELSRDLFVCEDAGDLDICL
                     ITPDGEVSRFVHLDGVQHGDPNTDASSETTGPSFDPSGTRLYFSSQRAFGFGVIYEVT
                     GPFRRLPGPAPAPGGAAPLELRIHAYRRTLKSRLASQGFRVGVALTKAADVTVVLRAP
                     VGAKGAPVTLAVGSRRGMAAGKGKLTLSLTRKGKGYLKRVGRPVTATIVATATTAGGE
                     RDVVERPMALR"
BASE COUNT         1516 a         3644 c         3845 g         1405 t
ORIGIN      
        1 ggttgtttca tgttgcgaaa ctagaaacaa cggctaaact gacggacgcc gtgttgaagg
       61 gccaagacat agtgctgctg ttgaagctcg cgcgcgacga cggagactgg accatgcggt
      121 cactgggcga ggccctagac ctcgacccgg cttcggtcca ccgagcactg cgacgcctcg
      181 atgaggcacg cctgattgac atcaaacgcc ggcgagtcaa tcgtggaagg gctgaagagt
      241 ttctgctgca tgggttccag tacgtctttc ccgtccacca gaaaggcacg agccgaggac
      301 tccctacggc ctgggcggcg ccaccgctca gcgacgaact ggctttgccg gacgacccac
      361 cgcctgtctg gccagacgcc aagggaaaga cgcgaggcgt ggcggtcgag ccgatccacg
      421 cgagcgtgcc ggccctcgca cgtcgcgatc ccgaaatggg cgaacgactc gcgttgctcg
      481 acgggattcg cctgggagac gcgcgggtgc ggaaactggc gagccagcat ctcgtgcggc
      541 gtttggacgc gggcaattga gcatcgggct gctagagcgc gccggcgcgg acttgggtgc
      601 cctcgttgac gaggtcgtgt tcgtcggtgg ggcgaccctc gccctgtgga tcactgatcc
      661 gggtgcgccg ccgatccggc cgaccaaaga cgtggacgtc atcgtcgaag tcaccacacg
      721 cggtggctac tacgaatttg aagaacgcct acgcgcggcg agctttgcgc cggaagggag
      781 cgtgatctgt cgctggattc aacgtgacac ggcactcatc gtcgatgcga tgcccgccga
      841 cccggcaatc ctcgggttct caaaccggtg gcagagagcg gcactcccat tcgcggcgga
      901 ggtgcagttg ctctcaggtg cgcgaattcg cgccgttcca ccgccgttcc tcctcgccac
      961 gaaagtcgag gcgttcggtg ggcgcgggca ggacgatttc ctcggtagcc gcgatttcgc
     1021 cgacatcgta agcctcgtgg acggccgcga gcagatcgtc gacgaagtac ggacagcgcc
     1081 gccgaaagtt cgttcgtatc tggcggacga gctgagcagc ctaatgagtc gtgcgcggtt
     1141 cgccgaggga gtctccgcgc agctgccacc ggatgaggcg agccaagagc gggccgatct
     1201 ggtggttgct ccgcggctgc gggcgatcat cgacgcggca taggacaggg gacgactcaa
     1261 aggccaacgc gtcactgcga acgggcactc gaacgcgatg accgaggtcg cgaacgagcg
     1321 cctgaccgac gacgtcgcgc ggtcctgccg gttctacgtc gagaagctca tcagtccctg
     1381 cggcgacgag cggcggtagc gcctactcgg cgggcttgtc ggtctcgcgc cgcgcgcgga
     1441 acagccgcac cggctcgccc acgcccttca gcttgcgacg ccctgccggc gaccacctga
     1501 agccatcgcc agctgcctcg cgtgcgtcgg cggtgcagag cacgctgccc ggccgcgcca
     1561 cgccggtcac gcggctggcc gtgttcaccg ggctgccgta ccagtcgccc gcgcgctcga
     1621 gcgccggtcc gcgcgcgatg ccggagcgca gcgccgggaa ctcgtcgccg gcctcggcgg
     1681 cgcgctcgac cagctcgagc gcgacctcga ccacggcgtc ggtctcgcgc cccaccagca
     1741 tcgcggcgtc gccgatcgtc ttcaccagcc gcaccggcgg gcgggcgacg tcacccgcga
     1801 gatccgtcag ccgcccggcc acgcggctga ggtcctccgg cgggatctcc tcgcccagcc
     1861 gcgtgaagcc gacgaggtcg gcgaagcaca ccgccacctc ctcggtgccg tcgagcctgc
     1921 ccgtggcaag ctcggcgcgg ctgacgatgt cgttgcgcac gagctcgcgc aggtgcaggc
     1981 cgtagacgta ctggagctcc gacccgatga ccgggatgag gtgctcggcc gcggcggcgt
     2041 agcgcagacc ggcgtcgcgc tcggtgtcgc ccggccgggc cagcgcgcgg ccgacgatct
     2101 ccaccgacgc gtcggcaacc cggcgcatgc cctcgcccat gatccgggcg atctgggtga
     2161 tgccctcctc cggcagcccg gcgtcgatga actgcttgag ctgccgcgcc ccctcgaggt
     2221 cgctgtcgcc gaagatcttc gcgtcctggt ccgacaccgg gagccccagt gcctgccact
     2281 ggcgctcgag cacctcgatc gggagcccga cctccgcggc gatctcgttg ccgctgaact
     2341 tcccctgccc gccgagcacc tgctcgaccg gcagcagggt gagccgctcc tcggccaccg
     2401 ccttcttcat ctcgtcgagc tcgacgcccg attcgtgaag ctgttcgagc aggtcgagcc
     2461 gggccgcccg ggcgccgtct tcgagcccgt cgagcagccc ctcggcctcg aagtcggggg
     2521 cggctacgcg ccctcccgca gaagctcgcg cagacgctcc tccggcagca ccgagggatc
     2581 cgtctcggcc atgaagtcga gcagctcgtg cgtgaagcgg cgcgggtcgt cgcggtgcgg
     2641 gaagtgcccc gcgttcggga acgccgcgag gcggctgccg ggcatcgagt cgctcgcccg
     2701 caggccatgc gcgaacggga tgaccgggtc gcgctcgccc cagacgatca gcgacggcat
     2761 ctcggccgcg agatagagcc ggtcggtcgc gctcacgcgc tggccgccgg ggtcgatgat
     2821 gccgcgcagc gtgtggatga aggccgcgcg cgcctcgacg tcgcatagcg agctgaagcc
     2881 acgccagatc tcctcgaggt cggcgccggc acgcaggccg aggccgccga cggcgccggc
     2941 gatcgagttt gtccactcga cgatcggccg cgcggtgagc agcgggagca cgaactccga
     3001 gccgggcagc gccgctgcgc gcagcaggat gttgacctcg cggccgaggc cgccgctcga
     3061 gacgagaacg aggcgctcga cgcgctcggg gaactggtag gccatctgca tcgccacgcc
     3121 gccgccgagc gagtgaccga ccaccgtggc gcggtcgtgg ccgagcgcgg cgagcaggtc
     3181 gcggatgccg ctggcgtagg caccgagcga gtagtcgccg cgcggcttgg cggactcgcc
     3241 gtggccgagc aggtcgggcg cgaccaccgt gtggtcctcg gccagctcgt cgatcacgtc
     3301 gagccaggtg tcggagctgc cggtgatgcc gtggatcagg acgatcaccg ggccccagcc
     3361 ggcctgccgg tagctgaccc ggtggccgtg cagagttatc tcttcgcgtt ccattggtct
     3421 cctagagggg aagcgtacgc cggtcggtca ggcgggtgtc gcggccccgc ggccgctgcg
     3481 tgcgcgagct ccaccgaggc gcgcgagcgc gacggtgagc gcggcgatca tcgcgcttgc
     3541 gatggccagc acggtcaggt agccggcgag cgtgagccag cccggcacgc cgtccttctg
     3601 ctcgcgctgg aggttcttcc tgtcgagcgc gaaggcacgc tcgaagcggg gcgacgcggg
     3661 cacctccgcg gccgggatcg cggtatccct gggcatgaag atcggcacgc cgacgatccg
     3721 gttgccgctg tgcaggcgaa ggatcgcctt ccagctcccg tccaccggga tcgggccggc
     3781 gctgcgatac gtgccgggcc cgacctcgcg catgcggacg atcctgctgc cgccgccctg
     3841 ccaggcggtg acgttgagcc actcggcgcc gtcggcggcg tccggcgggt cgagggtggc
     3901 ggtggccacg acctcgcggt tgggtgcggc acgcacctcg tcgagcgtga cgcgcgccgt
     3961 tgtcggggtg ccctccgaga ccggcagcgt gtagccgagc gcagcgacgg cggccacagc
     4021 cgcggcggcc agcgcgaatc gctcgggccg ggtcacgggg ttggcgatgt cgttgctcag
     4081 cgcgcggccc atggcgccgc cgagcgcgcc cgcggcgacc gccgaaacga acccgagcag
     4141 ggcggcctcc ggcagcagcg cggtgggcca ctcgatcggc atccagacgt gcgaccagcc
     4201 ccactcggcg gcgaggccga gcgtgccgat gcccacgccg gcgagggcgc cgaagcggac
     4261 cgcgcgatcg gtgctcacgc gcagcgcgac gagctcgacc gcgagcgcgg cgccgaggta
     4321 gagagggaag tgcggggtgg tctgtccgaa gacgggcccg acgagcaggg acacgaggcc
     4381 gcggaccgcg aggtacgaga gcgccgcgat cagcgcgccg ccacggccga tccggacgcg
     4441 tgccacgacg agggcgatcc cggccgcgag catcagcagg atcgggtgga agaggaggcg
     4501 gaactgcggc acgccgaagt cgaactcggc ctggaacgtg gagaggccga cgagcagtcc
     4561 gccggcgacc gcggcctggc ggagcttcag gagcggcagc ctgcgtaccc gcgggccgcg
     4621 gcgggccgta ccctccacga agaggaccca ggccgagatg gtcgccagcg cggccccgcc
     4681 gatcagcatc aggtgagtgg ggccccagag ggtcacgtcc tggccgaaga ggcggtgcca
     4741 gaagtcgtcc agcgggaagc cggcgagcga gaagacgccg cagccgacca tcagcacgcc
     4801 gcccagcggc gcgtgccagc ccggggcgat ccgcaccgcg acgtcgctcg gcttctcacg
     4861 cggcaggcag acggcgacga ggccggcgaa gagcacgccg aagaggccgg cgaggatcag
     4921 gtagtgcgcg gggttggcga gcggcccggg gtcgcggccg ttgtcgatgt gcagcgagat
     4981 gtcccagtac atgccgagca gggcgacgac cagcgagacc cccgagatca tcgaagggag
     5041 ggcggcccag cccggaaggc cggtctgccg cgcggtgaag tcggcggcgc gggcgagcac
     5101 gccgtcgcgg ccgttgcggt ggggtacgag gacggcgagc atcagcgccg tgagcacgag
     5161 cgcgccgacg gtggccccga tggcctcgcc ggtcgcggcg cccccggcgg gctcggtggc
     5221 cgccgcaagc gggatgatct gcgtcatggc cgacatcgta gtatctaact gaccgttcag
     5281 tcagttcagg aaacgtgagt tcgagcacgg aagacaccag ggcggcgaag gccgcacgcg
     5341 ggacccggtc gggcgcgaag cccgcgcggg gtggagcgag cgcgaaggcg ccgccggccg
     5401 gtggcgacaa gcgcggcggc tccgccccac cccgccgccg gctctccgcc gagcagcggc
     5461 gcgcggcgat cctcgacgcc gctctcgagg tcttcgccaa gcacggctac cacgcggcct
     5521 cgatcgacga catcgccggc gtggcgaccg tctccaaggc cctcatctac gagcacttcc
     5581 cctccaagcg ccacctgcac gccgcgctgc tcgagcgcca cgtcggcgag ctgttcgggc
     5641 tgctggtcga gagcgcggcc acctcggagc ccggcgaggt gcgcctgcgc gcgggcgtgg
     5701 acgcgttcct ggccttcgtc gagacgcggc ccgacgcgtt ccggatgctg ttccgcgacg
     5761 ccgtcgagcc ggaggtggcc gaggtcctgc gcacgctcgg gcagcaggcg gcccgccagg
     5821 tggccgagct gatcgccacc gagccgcgcg agcgccgcgc cgacgacgag gaggaggacg
     5881 aggcgatcga gatgctgggc gcgctgctca ccggcgccgt ccagtcgctg gcgctctggt
     5941 gggacgagca cccgaaggtg ccgcgcgaac ggctcgtcga gcgcaccatg gagttcgcgt
     6001 ggctcgggtt cgagcgcctg cgcgcgggcg agcgcctgaa ggagacggca gcatgaggtt
     6061 cggcctcttc tacgagcacc aggaaccgcg gccctgggag gagggctcca ccgagcggct
     6121 gctcaacgac gcgctcgacc aggtcgagct ggccgaccgc gtggggttcg actacgtctg
     6181 ggaggtcgag caccacttcc tcgaggagta ctcacactcg agcgcgcccg aggtcttcct
     6241 cgcggccgcg agtcagcgaa ccgagcgcat ccgcctcggt cacgggatcg tccagctccc
     6301 gccggccttc aaccaccccg tgcgcgtggt cgagcgcgcc gccacgctcg acctgatctc
     6361 gggcgggcgc gtggacctgg gcacgggcga ggccagctcg caggtcgagc tgggcggctt
     6421 cggcgtcgac cgcaacgcca aacgggcgca gtgggaggag tcgatcgacg ccgtcgcgcg
     6481 catgttcgtc gaggagccgt tcgcgggcct cgacggcgag cacgtgagca tcccgccgcg
     6541 caacgtgatg cccaagccga agcagaagcc gcacccgccg ctgtgggtcg cgtgcagccg
     6601 ccgcgagacg atcatcatgg ccgcgcggcg cggcatcgga gcgctctcgt tcgcattcat
     6661 cgagcccgag gccgcgaagg agtgggtgga cgagtactac gcgctgatcg agtccgacga
     6721 gtgcgtgccc gctggcttcg acgtgaacgc ggacttcgcg gtcgtgctcc cgatgatgct
     6781 ccacgaggac gaggccaccg cgatcgagcg cggcatcgac ggcgcccact tcttcggcta
     6841 ctcgctcgct cactactacg tgttcgggga gcacgtcccc gggcgcacga acatctggga
     6901 ggagttccag cgcgaccgct cggagaaggg cttcgcccgc gagatcgtct cgccggacga
     6961 cgagccgctc ggcgtgaagc tgctccagca ggggatgggc tccctgcgcg gggcggtcgg
     7021 cacgcccggc caggtcgagg agctgtgccg ccgctatgag gccgccggag tggaccaggt
     7081 gatcttcgtg ctccaggccg ggcgcaaccg gcacgagcac gtgtgcgagt cgatcgagct
     7141 gttcggcgag cgggtgctgc ccggcttcgc cgagcgcgcg ggcgcgcgtg aggcggagaa
     7201 ggccgagcgg ctggcaggcg cggtcgagcg agcgaatgcg cgccgggagc cgccgcgccg
     7261 ggctccggac ggctacgtga tcacgccgca gtccgagccg gcgccggccg acgcgatgcg
     7321 ggcggcgaag cagcccgccc gtcccgcgga cgcggtcacc ggcggcggcg gtgtcgccca
     7381 gcgcctcggc aggatcggcg aggcggcgtt cgccgcgatg gtgcgcggtc gcagcgagga
     7441 gcagctcgag cgagcgctcg gcaacgacgt ggcgctgcgg ctcgtgttcg ggggcatgga
     7501 gcgcgcgttc gtgcccgagc gcgccgaggg cttcgagggc gcgatccagt acgagctcga
     7561 gtcgcaacgc ggcacccgcc gctggcacgt cgagatcgcc gacggccgcg cggtcgcgcg
     7621 gcccggcgcc accagcgacc ccgaggtgac actgcgcgcg caggtgcccg tcttcgcccg
     7681 catcttcgcg cgcgagctga accccgcgca ggcgctgctc gccggcgacc tggccgtgga
     7741 gggcgacttc atggttgccg gccggatcgg ctacatgttc ggcgaggact cgccgttctg
     7801 agcgagagcg cgcgcggtcg cctcgaccgg tagcttctac gcgccatgcg aggcatcgcg
     7861 cgcgacgtcc agcaccgcac cgagatctac ggcaccggca cggatgggaa cgtcagctcg
     7921 cgccacgtgc tcacgttccg ggtcgagcgc ccgggctcga ccccggtccc ggtgcagatg
     7981 aagggcatgc ggatcgaggg cgtgctcgcc gacggcgacg aggtctcggt cgagggcgag
     8041 cagcggcccg gcaaggtgat gaagaccaag cgcgtggaga acctgaccac cggggttccg
     8101 gtggtcgcgg gcgtgccgct ctacgtgaag atcgtcgcga tcccggtggt cgcggccgtc
     8161 atcgcgatgt tctcctacgt cgcgtacacc ttcgcgaccg ggccctagta cgagcgcggc
     8221 aggccgagcg agtgctcggc cacgtagttg aggaccatct cgcgcgagac cggcgcggtg
     8281 cgcgcgatgc ggaccgccca ccagaggtcc ggcaggccgt actcgtgcgt gaaggcgttg
     8341 ccgccgtggg tctggatcgc gcgatcgacg cagtggatgc cggcctcggc cgccgcgaac
     8401 ttggccatgt tgctcgcctc gccggccggc gcgcccgcgt cgtagagcgc ggcggccttc
     8461 tgcatcatca accgggccag ctcgagctcg atcttcgcct cggcgagcgg gtgcgagatc
     8521 ccctgatgcg cgccgatcgg cgtcttccac acctgccgct cgcgggcgta gtcggcggcg
     8581 agcgcgagcg cacggcgcga ggcgccgttg cagatcgcgg cgcccatgat gcgctcgggg
     8641 ttgagcccgt cgaacaccgc cttcagcccc gcggtctcgc cgccgaccag gcggtcgcgc
     8701 tcgatctcga cgtcgtcgaa gaacagcagc cactgcttgt ccgggccgga cgcgactgtc
     8761 acgacctcct gccgctcgag cccgggcgcg tcgctgtcga ccagcgcgat caccggcagg
     8821 ccgaggctgc cgtcgggctc gcgcatgcgc gcgaccacga ggatcgcgtc tgcgtcctcc
     8881 acccccgaga tgaacgtctt ctggcccttc agcgcgtagc cgccgtcggt gcgcacggcg
     8941 gccgtcgaga tgttgtgcga gttcgagccg gcgtcgggct ctgtgatcgc gaaggccacc
     9001 ttggtcgtgc cgcggccgat gccgcgcagc cagcgctcct tctggtcggg cgtgccatga
     9061 cggacgagga tgctgcccac gatcgcgggc gagaccacga tcaggagtag gcctacgccg
     9121 gccgcgctga tctcctcgcc caccgccgcg agcgccgaca tcccgagccc gccgccgtcg
     9181 tactcctcgg ggatgttcac cccgaggtag ccgcggctgg cgaggtcgtc ccacagctcg
     9241 gtgggcggct cgccggccag ccgcttctgg cgcgagtagg cggggccgta gcccgtgcag
     9301 atggccgcga ccgtctcgcg gatccgcgcc tcctcgtcgg aggggacgag ctggagtgcg
     9361 ggcggggtgg tcatcgcagt gccatgggac gctcgaccac gtcgcgctcg ccgccggcgg
     9421 tggtggccgt ggccacgatg gtcgcggtca cgggccggcc cacgcgcttc aggtagccct
     9481 tgcccttgcg cgtgagggag agcgtgagct tgcccttacc cgccgccatg cccctgcgcg
     9541 acccgaccgc gagggtgacc ggcgcgccct tggcgccgac cggtgcgcgc agcacgaccg
     9601 tcacgtcggc ggccttcgtg agcgcgacgc cgacgcggaa gccctgactc gcgaggcggc
     9661 tcttcagcgt gcgccggtag gcgtggatgc gcagctcaag cggcgccgca ccgccgggcg
     9721 ccggggccgg cccgggcagc cgccggaagg ggccggtcac ctcgtagatc actccgaagc
     9781 cgaacgcgcg ctgcgacgag aagtagagcc gcgtgccgga cgggtcgaac gaggggccgg
     9841 tggtctcgga gctggcgtcc gtgttcggat cgccgtgctg gacgccgtcg aggtgcacga
     9901 agcgcgagac ctcaccgtcc ggggtgatca ggcagatgtc gaggtcgccg gcgtcctcgc
     9961 aaacgaacag gtcgcgcgag agctcggaca ccgcgatgtt gtcggtgtcg gtcagctccg
    10021 gctcggggaa gtcgctctcc gagtagatgc gctcgtacgt ctctgtgcgc gggtcgtact
    10081 ggaacacgcg cccagcgctc gactcgacga agtagacgag cccggtgccc ctgtggacgc
    10141 acaacccctc gggccgggcc atcctgctgc tcgccacctg ctggcgggtg ggggtgctct
    10201 gggccagcgg gtccgggatc acgcgccact cgaccacgcc gttcgcgacg agggtcgcga
    10261 tctcgagcac gccctcgctc aggtcgcccc agcggcgcgg cgtgaagcgg tagaagccgc
    10321 cgcccgactg gtcctcggtc aggtacacgc gccggtcgct ctcgctgacg cacgcgtact
    10381 cgtgcgagaa cacgccgagc gcgggccgcg
//
LOCUS       DASVPL010000315        15605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_37773, whole genome shotgun sequence.
ACCESSION   DASVPL010000315 DASVPL010000000
VERSION     DASVPL010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15605
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37773"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VF517_18050"
     CDS             <1..1243
                     /locus_tag="VF517_18050"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8054892.1"
                     /translation="ARAERRARIASLAERAERDAETAAAAERAAAALAGAREAVAARR
                     DAAAAELAADEERGRATADELRACAREEAEIQGRLRRASEAVTATEVRAQQARDTNQE
                     RATELTRVAEALGHDPAAEIAAPLTEPLNPDERTELEVKVDRLARRREQLGPVNPLAQ
                     QEYEEAVEHVEELESQRSDLEQALAELETLIKETDRRIRESFEETFEAAARNFEDVVA
                     HLFPGGRGRLRLVKPDAGPRPVLGGEGAEPAESAEVGETVATEGPAVEAVTEADETAP
                     EPGDPGVEIEVTPAGKSTKRLSLMSGGEKSLVALAFMFAVFLAKPCPFYILDEVEAAL
                     DDANIDRFLQLLRRYCDRAQFIVVTHQKRTMDAADCLYGVSMAGNGVSKVISRRMPRA
                     EPGEDGAVSLAPADAEERSAA"
     gene            1254..2117
                     /locus_tag="VF517_18055"
     CDS             1254..2117
                     /locus_tag="VF517_18055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8054893.1"
                     /translation="MAAPMPQVDGVEHHYAQVNGFRMHYAEAGSGEPLILQHGWPQHW
                     WMWRHQIPPLAERYRVIVPDLRGYGWSEAPPRGYDKPQFAHDVVALMDELGIERARYA
                     GHDWGAVAGYHLAIDHAERFERIACLGAPPPWRKSPPPAQVVAVTIAYQGIISAPLLG
                     SLAVRNGFAETILKAGRQLGEFSAEEIRIYRDPLHEPGHDNASVQTYRTALTRELPGS
                     LRGGPSGRIGVPMLVMMGGAELFVKVLDQEVVLRHADAEVTFDIVEGSGHFLPEEVSA
                     HVTHALLDLFA"
     gene            2117..3109
                     /gene="ftsY"
                     /locus_tag="VF517_18060"
     CDS             2117..3109
                     /gene="ftsY"
                     /locus_tag="VF517_18060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX8054894.1"
                     /translation="MARSWRELFVLDAPAPEAEPDEDGAEQRRGFFRRLRENMSKSRQ
                     ALGAELQASVFQTLDDETWERLEETLIYADVGAKTTARIVERLETEAGSGDLAGGEDL
                     TRRLTELLADAARVGEDRIDLRPDPAVILVVGVNGTGKTTTIGKVAWHLKHELGQSVL
                     LAAGDTFRAAAVEQLVEWGERAGVEVVRAKEGSDPGAVAYDAVEAARARGHDVVIIDT
                     AGRLHTQQHLMDELRKVRKVIGRQIPEAPHETLLVIDATTGQNGLRQALLFGDAVKVD
                     GIVLTKLDGTAKGGIALAIAEEIGVPVKLIGIGEAIEDLRPFDPDDFARALLET"
     gene            complement(3130..5019)
                     /locus_tag="VF517_18065"
     CDS             complement(3130..5019)
                     /locus_tag="VF517_18065"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEX8054895.1"
                     /translation="MRGVIAAAGALVALACAGPAGAQAASEAPPRLKSIDSCGALARY
                     ARRHTQATIVARDAKQHGDRSLDPRAGEQGGGAGGGGDDAPDFSETNVQEQGIDEPDR
                     VKTNGVHLFVVGGDDGNVIHALDARSDRPGVVGSVELPGFDGEILLSGNRILALTWDD
                     GDPESWAGATILSEIDVSNPAAMRVLRTEKIDGEAISARLHGRVARVAIWTPPREVVV
                     EQPAGARWSAAYRRTRMRRAVRKAPLDTWRPSVVTADRTTGTRSERGAVPCGSVRRPR
                     EYSGSGMLTVRTVDLDRGLPAVDADGIFAEGETVYGGADGLYVATEEWIDPDTDFEDL
                     PSESVTAIHKFAIAGAETEYRASGSVPGYMLSQWSMSEHAGYLRTATTSEPPWFDEEP
                     RGESESQVVVLAERDGALVEVGRARGLGRGERIQAVRFMGDQGFVVTFREVDPLFTLD
                     LSDPHAPAVRGELKIPGYSAYLHPIAPGLLLGVGQDATARGELRGFQLSLFDVSDLAQ
                     PRRVAVHTIPGSSGSAAEWDHHAFLHWPATGLTLLPIEQYADDEDERASFSGAIGFKV
                     DPDARSIAEVGRVTRPAPEDELDAMPPERNVVIGERVFSVWDDGVRTSRLDDLSQTAW
                     VDLPR"
     gene            5083..6204
                     /locus_tag="VF517_18070"
     CDS             5083..6204
                     /locus_tag="VF517_18070"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF005758.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HEX8054896.1"
                     /translation="MSSRERTGDGTRAVHAGVPHPRQAAPPVPGPVFASYYHLEGDPE
                     GVDYVYGRYGNPTWTLLETALADLEGGPAVSFASGMAAASAVLLPLLRAGDVLVIPAD
                     GYPAVRALANGQLAECGVEVRAVPTAERISDEVLAGAKLVWLESPSNPGLEVTDIADV
                     VRRAGDALVAVDNTLATPLGQRPLELGADFSVGSDSKHLTGHGDLILGHVAAREQARA
                     DALAAWRTATGAIPGPFEAWLAHRSIATLELRLERQCGTAQALAERLLFRADVDAVRY
                     PGLTADPAHELARRQMHNHGTVVSFTLSDREHAERFLAACRLVAQGTSFGGVRSSAER
                     RARWGGNDVPDGFIRFSVGCETPEDLLADVEQALDASAP"
     gene            6219..6644
                     /locus_tag="VF517_18075"
     CDS             6219..6644
                     /locus_tag="VF517_18075"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX8054897.1"
                     /translation="MPDWALWLVIAAALVGGEILTLGFFLGPIAVAALLACLVAVVGG
                     SAGLQLAAFVAGSIASLAIVRPIAKRHMRTPPSIRTGTAALIGERAVVLERVDGDGGR
                     VKISGEVWSARAFDEDEVMEPGVRVEVMKIEGATALVAE"
     gene            complement(6700..9372)
                     /locus_tag="VF517_18080"
     CDS             complement(6700..9372)
                     /locus_tag="VF517_18080"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX8054898.1"
                     /translation="MRPPEAEHDDDSRLAVQVAVARVLAEATSVDDSSRAMLASIGST
                     LDWALGAMWEARDGLLHCDHVWHGDGVTASAFELASRGVAFEPGVGLPGRVWESASPA
                     WIRDVVDDANFPRAAVAAECGVHGAFAFPIRARRGVIGVVEFFTREVAEPDRYLLDLM
                     ETIGYQLGQLIERGRAEEAVRASEARKGAMLEASLDCIVSMDHDGNVVEFNAAAERTF
                     GYSSEEVVGLEMCDLIIPPALRDAHRNGLKRYLETGEGAVIGNRIEISAVSRDGREFP
                     VELTIVRAPLDGPPLFTGFIRDISERKRRQSFDRFMAEAGALLGASLDYETTLEAVAG
                     LAVPAIADWCAIDLLTDDGSVRRVAVAHSEPARAALLEEVARRWPTRLSDPYGSGKVI
                     RSGDPEHLAEVRESVLVSIAAGDHEHLEALRALGASSSVTVPLQTLPGRILGSLTLAG
                     TTARARFDAIDVALATELGRRCAVAVENARLYRERDRIARTLQQSLLPPDLPAIPGVA
                     IEARFRPAGAGIDMGGDFYDAFEVGDDSWAVTIGDVCGKGPDAAALTALVRYTLRAVT
                     MHERRPDRVLELVNRAVLRNRIDDRFCSAALAIVENRPQGVCVEIASAGHPLPLLLRP
                     DGTVEAIGARGHLLGLWPDFETEPLSLALEPGDALILYTDGVTDARAPERILDTDDLA
                     ELVRSCAGAGARATAEHIERAVSESLTVEPRDDIAVLVIEALGARAGADAAPADELDG
                     ALQLSIPSSTQAPARARRAIEALSGSLDPTLVDQLKLLATELVTNSCRHAAAKGDPIG
                     VVLAMDEQVVRLSVTDTGPGFSLPAGVAEPDDESGRGLFIVDALADRWGVTGDGGSCV
                     WIEMSLGGDATRTEASDDAWGADRSGGDRTGAPG"
     gene            9416..10339
                     /locus_tag="VF517_18085"
     CDS             9416..10339
                     /locus_tag="VF517_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8054899.1"
                     /translation="MDAGLIVLLVIAVVVLYTLARSIRIVPQARAGIVERLGRFYRTL
                     DAGLALVVPFIDRVRPLIDLREQVVSFPPQPVITEDNLVVSIDTVIYHQVTDPKAATY
                     EIADYIQGIEQLTVTTLRNVIGSMTLEDTLTSRDEINAQLRHVLDEATGKWGIRVNRV
                     EIKAIDPPASIQDAMEKQMRAERDKRAAILQAEGYKQSQILTAEGEKQSAILRAEGQR
                     ESQVLYAEGQSKAIETVFQAIHNGDADPKLLAYQYLQMLPQIAQGESNKVWIIPSEFT
                     SALGQLSSAIGSVGDGGDEPPRQVQSPPPGP"
     gene            10360..10872
                     /locus_tag="VF517_18090"
     CDS             10360..10872
                     /locus_tag="VF517_18090"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8054900.1"
                     /translation="MEDLDAIEALMAESIRAHFPAFYAEERVETAVRYIGKPDRMLVE
                     DGTYFVAEDETGLVACGGWSRRYRLYAGSGGDAEDARLLDPASEPAPVRAMFVRGDRS
                     RRGLGSRILELCEEAARAEGFTELTLMATLPGVPLYERHGFRRVQELDIPLEDGTLME
                     GVRMERAIVV"
     gene            10886..12217
                     /gene="ffh"
                     /locus_tag="VF517_18095"
     CDS             10886..12217
                     /gene="ffh"
                     /locus_tag="VF517_18095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX8054901.1"
                     /translation="MFDTLSDRLQSALSDVKGRGALTEQDVAKAMRQVRLALLEADVN
                     FQVVKQFTSQVKERASGAEVLDALNPGQQVVKLVDEELTALMGGAGRDLVFSSKPPTV
                     ILMAGLQGSGKTTACGKLARHLREEHGKSIGIAACDVYRPAAVDQLVKVGARAGATVY
                     EQGTERDPVDIASWALEQAKADGRDVLIVDTAGRLHIDADLMEELTRIRKAVKPHNVL
                     LVVDAMTGQDAVNVAERFAEVANFDGVILSKLDGDARGGAALSVKAVTGKPIFYASTG
                     EKLDAFERFHPNRMAQRILGMGDVLTLIEKAEQQFDEKEAVELERKIRKEQFTLDDFL
                     KQMQQVRKMGPLTNLLKMMPGAAGMGLGNLKVDERDLDRIQAIITSMTPEERAKPEII
                     KGSRRRRIAQGSGTTVQAVNQLLKQFGQMKKMMRQLQSGRMPDLQQLAGGR"
     gene            12281..12538
                     /gene="rpsP"
                     /locus_tag="VF517_18100"
     CDS             12281..12538
                     /gene="rpsP"
                     /locus_tag="VF517_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX8054902.1"
                     /translation="MRLTRVGSKKNPVWRVVVADQRSPRDGRVIEHVGRYNPQTEPSE
                     IVLDEDRLQHWIARGAQPTDTVKKLMKIHARGAAESPSAEE"
     gene            12546..12779
                     /locus_tag="VF517_18105"
     CDS             12546..12779
                     /locus_tag="VF517_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX8054903.1"
                     /translation="MKDLVEYLARALVDLPDEVVVEEFEEDDGTVVYEVTVAGDDVGK
                     VIGRNGRTVNALRTVIRAAAVRENRRVLVDVVD"
     gene            12776..13201
                     /locus_tag="VF517_18110"
     CDS             12776..13201
                     /locus_tag="VF517_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054904.1"
                     /translation="MRPAGRVGRAHGYDGSFYVNGADSEFAVGTAIRIGSVDAVIDRR
                     AGTDARPLVRAPGVDPRDHVHQAILVDEPLAEDEYAAPDLVGCEVPGVGTVARVVNGP
                     SCDVLEVGDVLIPFIADAVRSVDLERRVIEVDRGFLGLP"
     gene            13198..13917
                     /locus_tag="VF517_18115"
     CDS             13198..13917
                     /locus_tag="VF517_18115"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054905.1"
                     /translation="MNIDIFTLFPEWFEWFGGQRHVRNSGVRIDAVDLRVSTPLKGGR
                     VDDTPYGGGAGMVVRVDVIEAALHARYGEDPRGRRVIALAAGGRQFDDALAGELAGEL
                     AAAAPAEELEFPPDRGLTLLCGRYEGFDERVHTGLATDVVSIGPYVLAGGELAAMVVC
                     DALIRKLPGALGDESSAVEESFSEALEGAPEYPHYTRPLEWRGERVPEVLLSGNHAQI
                     AAWRREQSRARGAARGGPDRV"
     gene            13986..14318
                     /gene="rplS"
                     /locus_tag="VF517_18120"
                     /pseudo
     CDS             13986..14318
                     /gene="rplS"
                     /locus_tag="VF517_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            14672..15328
                     /gene="lepB"
                     /locus_tag="VF517_18125"
     CDS             14672..15328
                     /gene="lepB"
                     /locus_tag="VF517_18125"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8054906.1"
                     /translation="MTSGQDEDREQEEGGGRKRSGAGSAIEFVLIVVVALGLALGIQA
                     FLVKPFRIPSASMEPTLDIGQRVLVSRVNYHVGEPDRGEVVVFKPPAGADRNECGVPG
                     FEPASRNQSCPRPTPGRSDQNFIKRIVAVGGDRVRIERGRVILNGKPVDEPFIRPSED
                     CQICNLPKEIRIPPKHYFMMGDNRGESADSREWGPVPEDQLVGQAFFTYWPPKRIGTL
                     "
     gene            15407..>15605
                     /locus_tag="VF517_18130"
     CDS             15407..>15605
                     /locus_tag="VF517_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054907.1"
                     /translation="MMDPAAATSPAALEEAPEMGALPATAPAPLAEAAAPAEVPPAPA
                     EAAPPSVAEAAPSAEAPPAPAA"
BASE COUNT         2195 a         5688 c         5695 g         2027 t
ORIGIN      
        1 cgcccgcgcc gagcgccgcg cccgcatcgc ctcgctggcc gagcgcgcgg agcgcgacgc
       61 cgagacggcc gccgccgccg agcgcgccgc cgccgctctg gccggcgccc gcgaggccgt
      121 ggccgcccgg cgcgacgccg ccgcggccga gctggcggcc gacgaggagc gcggccgcgc
      181 gaccgccgac gagctgcgcg cctgcgcccg cgaggaggcg gagatccagg gccgcctgcg
      241 cagggcgagc gaggcggtaa ccgccaccga ggtccgcgcc cagcaggcgc gcgacacgaa
      301 ccaggagcgg gccaccgagc tcacgcgcgt cgcggaggcg ctcggacacg accccgcggc
      361 cgagatcgcg gcccccctaa ccgagccgtt gaacccagac gaacgcaccg agctcgaggt
      421 caaggtcgat cgcctggccc gccgccgcga gcagctcggc ccggtcaacc cgctcgctca
      481 gcaggagtac gaggaggccg tcgagcacgt cgaggagctc gagtcgcagc gctcggacct
      541 cgagcaggcc ctcgccgagc tcgagaccct gatcaaggag accgaccggc gcatccgcga
      601 gagcttcgaa gagaccttcg aggccgccgc gcggaacttc gaggacgtcg tggcgcacct
      661 cttccccggc ggccgcggcc gcctgcgcct cgtgaagccg gacgccggcc cgcgccccgt
      721 gctcggcggc gagggcgccg agcccgccga gtcggccgag gtcggcgaga ccgtggccac
      781 cgagggcccc gcggtcgagg cggtcaccga ggccgacgag accgctcccg agcccggcga
      841 ccccggcgtg gagatcgagg tcacgcccgc cggcaagtcg acgaagcgcc tgtcgctgat
      901 gtcgggcggc gagaagtcgc tcgtcgccct ggcgttcatg ttcgccgtgt tcctcgcgaa
      961 gccgtgcccg ttctacatcc tcgacgaggt cgaggccgcg ctcgacgacg ccaacatcga
     1021 ccgcttcctc cagctcctgc gccgctactg cgaccgcgcc cagttcatcg tcgtcaccca
     1081 ccagaagcgc acgatggacg ccgccgactg cctgtacggg gtgagcatgg ccggcaacgg
     1141 cgtctcgaag gtgatctcgc ggcggatgcc gcgcgcggag ccgggcgagg acggcgccgt
     1201 cagcctcgca ccggcggacg cggaggagcg cagcgccgcg taaggtctgg ggcatggccg
     1261 cgcccatgcc ccaggtcgac ggcgtagagc accactacgc ccaggtgaac ggcttccgca
     1321 tgcactacgc ggaggccggc agcggcgagc cgctgatcct ccagcacggc tggccgcagc
     1381 actggtggat gtggcgccat cagatcccgc cgctcgccga gcgctaccgg gtgatcgtcc
     1441 ccgacctccg tggctacggc tggtcggagg ccccgccgcg cggctacgac aagccccagt
     1501 tcgcgcacga tgtggtggcg ctcatggacg agctcgggat cgagcgggcg cgctacgcgg
     1561 gccacgactg gggcgcggtc gccggctacc acctcgcgat cgaccacgcc gagcgcttcg
     1621 agcgcatcgc ctgcctgggc gcgccgccgc cgtggcgcaa gagcccgccg ccggcgcagg
     1681 tggtcgccgt caccatcgcc taccagggga tcatctcggc gccgctgctc ggctcgctgg
     1741 cggtgcgcaa cgggttcgcc gagacgatcc tcaaggccgg caggcagctc ggcgagttca
     1801 gcgccgagga gatccggatc taccgcgatc cgctccacga gcccggccac gacaacgcgt
     1861 cggtgcagac ctaccgcacg gccctgacgc gcgagctgcc ggggtcgctg cgcggcggac
     1921 ccagcggccg catcggcgtg ccgatgctcg tgatgatggg gggcgcggag ctgttcgtga
     1981 aggtgctcga ccaggaggtg gtcctgcgcc atgccgacgc cgaggtcacc ttcgacatcg
     2041 tggagggctc ggggcacttc ctgcccgagg aggtctcggc ccacgtcacg cacgcgctgc
     2101 tggacctctt cgcctgatgg cgcgtagctg gcgcgagctg ttcgtcctcg acgcgccggc
     2161 ccccgaggcc gagccggacg aggacggcgc cgagcagcgc cgcggcttct tccgccggct
     2221 gcgcgagaac atgtccaaga gccgccaggc gctgggcgcg gagctccagg cgagcgtctt
     2281 ccagacgctg gacgacgaga cctgggagcg cctggaggag acgctgatct acgcggacgt
     2341 gggggcgaag acgaccgcgc ggatcgtcga gcggctcgag accgaggctg ggtccggcga
     2401 cctcgcgggc ggcgaggacc tgacgcgccg ccttaccgag ctgctcgccg acgcggcccg
     2461 cgtgggggag gaccgcatcg acctgcggcc ggatcccgcc gtgatcctcg tcgtcggcgt
     2521 gaacgggacc ggaaagacca ccacgatcgg gaaggtcgcc tggcacctca agcacgagct
     2581 cggccagtcg gtcctgctcg ccgccgggga cacgttccgc gcggcggcgg tcgagcagct
     2641 cgtggagtgg ggcgagcgcg ccggggtcga ggtggtcagg gcgaaggagg gatcggaccc
     2701 gggcgccgtc gcgtacgacg ccgtcgaggc cgcccgcgcg cgcgggcacg acgtcgtgat
     2761 catcgacacc gccggccgac tgcacactca gcagcacctg atggacgagc tgcgcaaggt
     2821 ccgcaaggtg atcggccgcc aaatcccgga ggcgccgcac gagacgctgc tcgtgatcga
     2881 cgcgaccacc gggcagaacg gtctgcgcca ggcgctgctg ttcggcgacg cggtgaaggt
     2941 ggacggcatc gtgctcacga agctcgacgg cacggccaag ggcggcatcg cgctggccat
     3001 cgccgaggag atcggcgtgc cggtgaagct gatcgggatc ggcgaggcga tcgaggacct
     3061 gcggccgttc gaccccgacg acttcgcccg cgcgctgctt gagacctgat cagcgcggct
     3121 ggagcctgat cagcgcggaa ggtcgaccca ggcggtctgc gacaggtcgt cgaggcgact
     3181 cgtccgcacg ccgtcgtccc acacgctgaa cacccgctcg ccgatcacga cgttgcgctc
     3241 cggtggcatg gcgtcgagct cgtcctctgg ggccggcctc gtgacgcgcc cgacctcggc
     3301 gatgctgcgc gcgtccggat cgaccttgaa cccgatcgcc ccggagaagc tcgcccgctc
     3361 gtcctcgtcg tcggcgtatt gctcgatcgg cagcagggtc agtccggtcg ccggccagtg
     3421 gagaaaggcg tggtggtccc actcggcggc ggacccggag ctacccggga tggtgtggac
     3481 cgctacacgc cggggctgtg ccaggtccga gacgtcgaag agcgatagct ggaacccgcg
     3541 cagctcgccg cgcgccgtcg cgtcctgtcc gacgccgagc agcaatcccg gcgcgatcgg
     3601 gtggaggtac gccgagtagc cagggatctt cagctcgccc cgaaccgccg gggcatgcgg
     3661 gtccgacagg tcgagcgtga agagcggatc gacctccctg aacgtcacga cgaacccctg
     3721 gtcgcccatg aagcgaaccg cctggatccg ctcgccgcgg cccaggccgc gggcgcggcc
     3781 gacttccacc agcgcgccgt cgcgctcggc gagcaccacg acctggctct cggactcccc
     3841 gcgcggctcc tcgtcgaacc agggtggctc gctcgtcgtg gcggtgcgca ggtacccggc
     3901 gtgctccgac atcgaccact ggctgagcat gtagccgggg accgagccgc tcgccctgta
     3961 ctcggtctcg gcgccggcga tggcgaactt gtggatcgcc gtcaccgatt cgctcggcag
     4021 atcctcgaag tccgtgtccg ggtcgatcca ctcctccgtg gcgacataga gaccgtcggc
     4081 gcccccgtac acggtctcgc cctccgcgaa gatgccgtcg gcgtcaacgg ccggcaggcc
     4141 gcggtcgagg tccaccgtcc gcacggtgag catcccgctg ccggagtact cgcgcggccg
     4201 cctcaccgac ccgcacggca cggctccccg ctcgctgcgc gttccggtgg tgcgatcggc
     4261 cgtcacgacg ctcgggcgcc aggtgtcgag cggcgccttg cgcaccgcgc gccgcatgcg
     4321 cgtacggcgg tacgcggcgc tccagcgcgc gccggccggc tgctcgacca cgacctcgcg
     4381 cggcggcgtc cagatggcca cgcgcgccac ccggccgtgc agccgagcgc tgatcgcctc
     4441 gccgtcgatc ttctcggtgc gcagcacgcg catcgcggcg gggttcgaga cgtcgatctc
     4501 ggagaggatc gtggcccccg cccagctctc cgggtcgccg tcgtcccacg tgagcgcgag
     4561 gatgcggtta ccgctgagca ggatctcccc gtcgaagccg ggcagctcga ccgatccgac
     4621 gaccccgggg cggtcggagc gcgcgtcgag tgcgtggatc acgttgccgt cgtctccgcc
     4681 gacgacgaac agatggaccc cgttcgtctt cacgcggtcc ggttcgtcaa tgccctgctc
     4741 ctgcacgttg gtctccgaga agtcgggcgc gtcgtcaccg cctcctcccg caccgccgcc
     4801 ctgctcgccg gcacgcgggt ccagggaccg atccccatgt tgcttcgcgt cgcgggccac
     4861 gatcgtggcc tgcgtgtggc ggcgggcgta gcgcgcgagc gctccgcacg agtcgatcga
     4921 cttgagccgc ggcggcgcct cgctcgccgc ctgcgcgccc gccggcccgg cgcacgcgag
     4981 cgcgaccagc gccccggcag cagcgatcac ccccctcata ccccggtgaa caccgcatcg
     5041 gcgcgagggg tgcggtgtcg gccaagcggc tttactgcac gcatgagctc acgcgagagg
     5101 accggcgacg gcacccgtgc cgtgcacgcg ggcgtgccgc atccgcgcca ggccgcaccg
     5161 ccggtcccag gcccggtgtt cgcgagctac taccacctgg agggcgaccc ggagggcgtc
     5221 gactacgtct acgggcgcta cggcaacccg acctggacgc tgctcgagac cgcgctggcc
     5281 gacctcgagg gcggcccggc ggtgtcgttc gcgagcggca tggcggcggc atcggcggtg
     5341 ctgctgccgc tgctgcgcgc gggcgacgtt ctcgtgatcc cggccgacgg ctatccggcc
     5401 gtccgcgcgc tcgcgaacgg tcagctcgcc gagtgcggcg tcgaggtccg cgccgtgccc
     5461 acggccgagc ggatatccga cgaggtgctg gcgggcgcga agctcgtatg gctcgagtcc
     5521 ccgtcgaacc ccggcctcga ggtgaccgac atcgccgacg tcgtgcgccg cgctggcgat
     5581 gcgctcgtgg ccgtcgacaa caccctcgcc acgccgctcg gccagcgccc gctcgagctg
     5641 ggcgccgact tctcggtggg cagcgattcg aagcacctca ccggccacgg cgaccttatc
     5701 ctcggccacg tggccgcgcg cgagcaggcg cgggcggatg ccctggccgc gtggcggacc
     5761 gccaccggcg ccatcccggg accgttcgag gcgtggctgg cgcaccgctc gatcgcgacg
     5821 ctcgagctgc gtctcgagcg ccagtgcggc acggcgcagg cgctggccga gcggctgctg
     5881 ttccgagcag acgtggacgc ggtccgctac cccggcctga ccgccgaccc cgcgcacgag
     5941 ctcgcgcggc gccagatgca caaccacggc acggtggtct cgttcacgct gtcggaccgc
     6001 gagcacgccg agcgcttcct ggcggcgtgc cggctggtgg cccagggcac gagcttcggc
     6061 ggcgtgcgct cgagcgccga gcgccgtgcc cgctggggcg gcaacgacgt gccggacggc
     6121 ttcatccgct tcagcgtcgg ctgcgagacg ccggaggacc tgctggccga cgtggagcag
     6181 gcgctcgacg cgtcggctcc ctgacatacg attcccgcat gccggactgg gcactgtggc
     6241 ttgtgatcgc ggcggcgctc gtgggcgggg agatcctcac gctcgggttc ttcctcggcc
     6301 cgatcgcggt ggctgcgctg ctcgcgtgcc tcgtggcggt ggtcggcggc tcggccgggc
     6361 tccagctcgc cgcgttcgtg gcggggtcga tcgcatcgct cgcgatcgtg cggccgatcg
     6421 ccaagcgcca catgcgcacc ccgccgtcga tccgcacggg cacggccgcg ctgatcggcg
     6481 agcgcgcggt cgtgctggag cgcgtcgacg gcgacggcgg ccgcgtgaag atcagcggcg
     6541 aggtgtggag cgcccgcgcc ttcgacgagg acgaggtgat ggagcccggc gtgcgcgtcg
     6601 aggtgatgaa gatcgagggc gcgaccgcgc tcgtcgcgga gtagccgcgc agagcggcgc
     6661 cgccgcggcc cccgcgccgc tcggttcggc gaccgcgccc taacccggcg cgccggtccg
     6721 gtcgccgccg ctgcgatcgg cgccccaggc gtcgtcgctc gcctcggttc gcgtcgcgtc
     6781 cccgccgagc gacatctcga tccacacgca gctcccgccg tccccggtca cgccccagcg
     6841 gtcggcgagc gcgtccacga tgaacagccc tcgccccgac tcgtcgtcgg gctcggccac
     6901 gccggcggga aggctgaacc ccggcccggt gtcggtcacc gacagtcgca cgacctgctc
     6961 gtccattgcg agcaccacac cgatcgggtc acccttcgcc gccgcgtggc ggcagctgtt
     7021 ggtcacgagc tcggtggcga gcagcttgag ctggtcgacg agggtggggt cgagcgatcc
     7081 cgacagcgcc tcgatcgcgc ggcgggcgcg cgccggcgcc tgcgtgctgc tcgggatcga
     7141 gagctggagc gcgccgtcga gctcgtcggc cggcgcagcg tccgctccgg cccgcgcgcc
     7201 cagggcctcg atcaccagca cggcgatgtc gtcgcgcggc tcgaccgtca gcgactccga
     7261 caccgcgcgt tcgatgtgct cggccgtcgc ccgcgcgccg gcccccgcgc aggagcgcac
     7321 cagctcggcc aggtcgtcgg tgtcgaggat gcgctcgggg gcgcgcgcgt cggtcacgcc
     7381 gtccgtgtag aggatcagcg cgtcgccggg ctcgagcgcg agcgacagcg gctcggtctc
     7441 gaagtcgggc cagagcccga gcaggtgccc gcgggcaccg atcgcctcga ccgtgccgtc
     7501 cgggcgcagg agcagtggga gcgggtggcc ggcgctcgcg atctcgacgc acacgccctg
     7561 cggccggttc tccacgatgg ccagcgccgc cgagcagaag cggtcgtcga tgcggttgcg
     7621 cagcacggcc cggttgacca gctcgagcac gcgatcgggg cggcgctcgt gcatggtcac
     7681 cgcgcgcagg gtgtagcgga cgagcgcggt gagcgccgcg gcgtcggggc ccttgccgca
     7741 cacgtcgccg atcgtgacgg cccacgagtc gtcgcccacc tcgaaggcgt cgtagaagtc
     7801 gccgcccatg tcgatgcccg cgcccgcggg acggaagcgg gcctcgatcg ccacgcccgg
     7861 gatcgccggc aggtccggcg gcaggaggct ctgctggagc gtgcgcgcga tccggtcgcg
     7921 ctccctgtag aggcgcgcgt tctcgaccgc cacggcgcag cggcggccga gctcggtcgc
     7981 gagcgccacg tcgatcgcgt cgaaccgcgc gcgcgcggtg gtgcccgcga gcgtgagcga
     8041 cccgaggatc ctccccggca gcgtctggag cgggacggtc accgacgagc tcgcgccgag
     8101 tgcgcgcagc gcctcgaggt gctcgtggtc gccggcggcg atcgagacga gcacgctctc
     8161 gcgcacctcg gcgaggtgct cgggatcgcc gctgcggatc accttgcccg agccgtacgg
     8221 atcgctcagg cgcgtcggcc agcggcgcgc gacctcctcg agcagcgcgg ccctggccgg
     8281 ctccgagtgc gccaccgcca cgcgccggac cgagccgtcg tcggtcagca ggtcgatcgc
     8341 gcaccagtcg gcgatcgcgg gcaccgccag ccccgccacg gcctcgagcg tggtctcgta
     8401 gtcgagcgac gcccccagca gcgcccccgc ttcggccatg aagcggtcga acgactggcg
     8461 gcgcttgcgc tcgctgatgt cgcggatgaa gccggtgaag agcggcggcc cgtcgagcgg
     8521 cgcgcgcacg atcgtcagct cgacggggaa ctcgcggccg tcgcggctca cggcggatat
     8581 ctcgatccgg ttgccgatca ccgcgccctc gccggtctcg aggtagcgct tcagcccgtt
     8641 gcggtgcgcg tcgcgcagcg ccggcgggat gatcaggtcg cacatttcca acccgacgac
     8701 ctcctccgag gagtagccga acgtgcgctc ggcggcggcg ttgaactcga cgacgttgcc
     8761 gtcgtggtcc atcgacacga tgcagtcgag cgaggcctcg agcatggccc ccttgcgcgc
     8821 ctcgctcgcg cgcacggcct cctcggcgcg gccgcgctcg atgagctggc cgagctggta
     8881 gccgatcgtc tccatcaggt cgagcaggta gcggtccggc tcggcgacct cgcgcgtgaa
     8941 gaactcgacc accccgatca cgccgcgccg cgcgcggatc gggaacgcga acgcgccgtg
     9001 cacaccgcac tcggccgcga ccgccgcgcg cgggaagttc gcgtcgtcca ccacgtcgcg
     9061 gatccaggcc gggctggcgc tctcccacac gcgcccgggg agcccgacgc ccggctcgaa
     9121 ggcaacaccg cggctggcca gctcgaaggc gctagcggtc acgccgtcgc cgtgccagac
     9181 gtggtcgcag tgcagcaggc cgtcgcgcgc ctcccacatc gcgccgagcg cccagtcgag
     9241 cgtgctgccg atcgacgcga gcatcgcgcg actcgaatcg tcgacgctcg tggcctcggc
     9301 gagcacccgc gccacggcca cctggaccgc gagccggctg tcgtcgtcgt gctcggcctc
     9361 aggtggtctc actacgcggc gggattgtgt cacgcgcagg tatgctgcgc gcacgatgga
     9421 tgccggcctg atcgtcctgc tcgtaatcgc cgtcgtggtc ctctacacgc tggcgcgctc
     9481 gatacggatc gtgccccagg cgcgagccgg gatcgtggag cgcctcggcc gcttctaccg
     9541 gaccctcgac gcgggcctcg cgctggtcgt cccgttcatc gaccgggtcc gcccgctgat
     9601 cgacctccgc gagcaggtcg tctcgttccc gccccagccg gtgatcaccg aggacaacct
     9661 ggtcgtctcg atcgacacgg tgatctacca ccaggtgacc gacccgaagg cggccacgta
     9721 cgagatcgcc gactacatcc aggggatcga gcagctcacg gtcaccacgc tgcgcaacgt
     9781 gatcggctcg atgacgctcg aggacacgct cacctcgcgc gacgagatca acgcccagct
     9841 ccgccatgtg ctcgacgagg ccaccggcaa gtgggggatc cgcgtcaacc gcgtcgagat
     9901 aaaggcgatc gacccgcccg cctcgatcca ggacgcgatg gagaagcaga tgcgcgccga
     9961 gcgcgacaag cgcgccgcga tcctccaggc cgagggctac aagcagtcgc agatcctcac
    10021 cgcagagggc gagaagcaga gcgcgatcct gcgcgccgag ggccagcgcg agtcgcaggt
    10081 gctctacgcc gagggccagt cgaaggcgat cgagaccgtc ttccaggcca tccacaacgg
    10141 cgacgccgac ccgaagctgc tcgcctacca gtacctccag atgctgcccc agatcgcgca
    10201 gggcgagtcg aacaaggttt ggatcatccc gagcgagttc acctcggcgc tgggccagct
    10261 ctcctcggcg atcgggtccg tcggcgacgg cggggacgag ccgccgcggc aggtccagtc
    10321 gcccccgccg ggcccctgac cgagctccgc cgcgcgcgga tggaggacct cgacgcgatc
    10381 gaggcgctga tggcggagtc gatccgcgcc cacttcccgg ccttctacgc cgaggagcgg
    10441 gtcgagaccg cggtccgcta catcggcaag cccgaccgaa tgctggtcga ggacggcacg
    10501 tacttcgtgg ccgaggacga gaccggtctg gtcgcgtgcg gcggctggag ccgcaggtac
    10561 aggctctacg ccgggtcggg cggcgacgcc gaggacgcgc gcctgctcga cccagcttcc
    10621 gagccggcgc cggtgcgggc gatgttcgtg cgcggcgacc gctcgcgccg cggcctcggg
    10681 tcgcgcatcc tcgagctctg tgaggaggcc gcccgcgccg agggcttcac ggagctcacg
    10741 ctgatggcga cgctgccggg cgttcccctc tacgagcgtc atggcttccg ccgggtccag
    10801 gagctcgata tcccgctgga ggacgggacg ctgatggagg gcgtccggat ggagcgggcg
    10861 atcgtcgtct agcctctccc gcggcgtgtt cgacaccctc tcagaccggc ttcagtccgc
    10921 gctctcggac gtcaagggcc gcggcgcgct caccgagcag gacgtggcga aggcgatgcg
    10981 ccaggtgcgg ctcgcactgc tcgaggccga cgtcaacttc caggtcgtca agcagttcac
    11041 ctcccaggtc aaggagcggg cttcgggcgc cgaggtgctc gacgccctga acccgggcca
    11101 gcaggtcgtc aagctcgtgg acgaggagct caccgcgctc atgggcggcg ccgggcgcga
    11161 cctcgtcttc tcgagcaagc cgcccaccgt gatcctgatg gccgggctcc agggctccgg
    11221 caagacgacc gcctgcggca agctcgcccg ccacctgcgc gaggagcacg gcaagagcat
    11281 cgggatcgcc gcctgcgacg tctaccgccc ggcggccgtc gaccagctcg tcaaggtggg
    11341 cgcgcgcgcc ggggcgacgg tctacgagca ggggaccgag cgcgaccccg tggacatcgc
    11401 gtcgtgggcg ctcgagcagg cgaaggccga cggccgcgac gtgctgatcg tcgacaccgc
    11461 cggccgcctc cacatcgacg cggacctgat ggaggagctg acgcggatcc gcaaggcggt
    11521 gaagccgcac aacgtgctgc tggtcgtgga cgcgatgacc ggccaggacg cggtgaacgt
    11581 ggccgagcgc ttcgcggagg tcgcgaactt cgacggcgtg atcctctcga agctcgacgg
    11641 cgacgcccgc ggcggcgccg cgctgtcggt caaggcggtc acgggcaagc cgatcttcta
    11701 cgcctctacg ggcgagaagc tcgacgcgtt cgagcgcttc cacccgaacc ggatggccca
    11761 gcgcatcctc ggcatgggcg acgtgctcac gctgatcgag aaggccgagc agcagttcga
    11821 cgagaaggag gccgtcgagc tcgagcgcaa gatccgcaag gagcagttca cgctcgacga
    11881 cttcctcaag cagatgcagc aggtccgcaa gatgggcccg ctcaccaacc tgctgaagat
    11941 gatgccgggc gctgccggga tggggctcgg gaacctgaag gtcgacgagc gcgacctcga
    12001 ccggatccag gcgatcatca cgtcgatgac ccccgaggag cgggccaagc cggagatcat
    12061 caagggctcg cggcggcgcc ggatcgcgca ggggtcgggg accacggtcc aggccgtcaa
    12121 ccagctcctc aagcagttcg gtcagatgaa gaagatgatg cgccagctcc agtccgggcg
    12181 gatgcccgac ctccagcagc tcgccggcgg ccgctagccc gccgcgcccc ggctcctcgc
    12241 cggcggccgc taggcttctg cccctcttat gagcgtccga gtgagactga cccgcgtggg
    12301 cagcaagaag aacccggtct ggcgcgtggt cgtggccgac cagcgctcgc cgcgcgacgg
    12361 ccgcgtgatc gagcacgtcg gccgctacaa cccgcagacc gagccgtcgg agatcgtcct
    12421 cgacgaggat cgtctccagc actggatcgc gcgcggcgct cagccgaccg acacggtcaa
    12481 gaagctgatg aagatccacg cgcgcggcgc ggccgagagt ccctccgccg aggagtagcg
    12541 cgaccgtgaa ggacctcgtc gagtacctcg ccagggcgct cgtcgacctt cccgacgagg
    12601 tggtcgtgga ggagttcgag gaggacgacg gcaccgtcgt gtacgaggtg accgtggccg
    12661 gcgacgacgt cggcaaggtg atcgggcgca acggccggac ggtgaacgcg ctgcgcacgg
    12721 tgatccgcgc cgcggccgtc cgcgagaacc gccgcgtgct cgtcgacgtc gtcgattgag
    12781 gcccgcgggc cgcgtcgggc gcgcgcacgg gtacgacggg agcttctacg tgaacggcgc
    12841 ggattcggag ttcgcggtcg gcaccgcgat ccgcatcggc tcggtcgacg ccgtgatcga
    12901 tcgccgggcc gggaccgacg cgcgcccgct cgtgcgcgca ccgggtgtgg acccgcgcga
    12961 ccacgtccac caggcgatcc tggtcgacga gccgctagcc gaggacgagt acgcggctcc
    13021 ggacctcgtc gggtgcgagg tgcccggcgt cgggacggtc gcgcgggtgg tcaacggacc
    13081 gtcgtgcgac gtcctcgagg tgggggacgt cctgatccca ttcatcgccg acgccgtccg
    13141 gtcggtggac ctcgagcggc gcgtgatcga ggtcgatcgc gggttcctgg gcctgccgtg
    13201 aacatcgaca tcttcactct gttcccggag tggttcgagt ggttcggcgg gcagcgccac
    13261 gtgcgcaact cgggggtgcg catcgacgcc gtcgacctcc gggtttccac ccccctgaag
    13321 gggggacgag tcgacgacac gccgtacggc gggggagcgg gcatggtcgt gcgggtggac
    13381 gtgatcgagg ctgccctgca cgcgcgctac ggggaggatc cgcgcgggcg ccgggtcatc
    13441 gccctcgcgg ccgggggccg gcagttcgac gacgcgctcg cgggcgagct ggcgggcgag
    13501 ctcgcggcgg ccgcgccggc ggaagagctc gagttcccgc ccgaccgcgg cctcacgctc
    13561 ctctgcggcc gctacgaggg cttcgacgag cgcgtgcaca ccggcctcgc caccgacgtg
    13621 gtctcgatcg ggccctacgt gctggccggg ggcgagctgg ccgcgatggt cgtctgcgat
    13681 gccctgatcc gcaagctgcc cggggcgctc ggggacgagt cgagcgcggt ggaggagtcg
    13741 ttcagcgagg cgctcgaggg ggcgccggag tacccgcact acacgcggcc gctcgagtgg
    13801 cgcggcgagc gcgtgcccga ggtgctgctc tcgggcaacc acgcgcaaat cgcggcctgg
    13861 cggcgggagc agagccgcgc ccgcggggcc gcgcgcggcg ggcccgatcg ggtctaggcg
    13921 cctcgctacc attgcgccgc tgagcgcgga cgcctcctcg taggtggtcg cgccctcttt
    13981 ttgccatgag ttcagtcatc gacagcatcg agcgcgccca gctgcgcacg gtcccccggt
    14041 tccaggccgg ggaccgcctc aaggtccact tccaggtggt ggagggcacg cggcgcagga
    14101 cgcaggtctt cgagggcgtc gtgctgaagc gccagggcag cggcgcgcgg gagacgttca
    14161 ccgtccgcaa gcagtcgttc ggcgtggggg tggagcgcac gttccccctg cattcgccga
    14221 agatcgagcg gatcgaggtg gccagccgcg gcgacgtgcg gcgggcgaag ctgtactacc
    14281 tgcgcggtcg cgtggggcgg cgcgcgcggg tgcgcgagcg ccagtacacg ggcccggagg
    14341 ccgacgccgc ggcgcgcgag gtcgcgctgg cgtccgacga ccagggcacg caggcggagg
    14401 ctcccgccga ggcgaacccg gacgcgacgg tcccggccga cggcacgacc gccgacggcg
    14461 aggcgcccca ggcccccgcc gacgacgcgc ccgacgcggc tccggccggc gaggccgccg
    14521 ccgattcgag cgacgacgcc acctcggtgc cgcctgactc ggaggccaag gagccacgcg
    14581 aggagccggc ccccgagggt gacgccgccg accaggcgct cgccgaggac ggctccgcct
    14641 cggaggactc gtcggaagag tccgccgggg catgacgtcg ggtcaggacg aggaccgcga
    14701 gcaggaggag ggcggcggcc gcaagcgctc aggcgctggg tccgctatcg agttcgtcct
    14761 gatcgtggtg gtggcgctgg ggctggcgct cggcatccag gcgttcctgg tgaagccgtt
    14821 ccggatcccg agcgcgtcga tggagccgac gctcgacatc ggccagcgcg tgctcgtctc
    14881 gcgggtgaac taccacgtgg gcgagccgga tcgcggcgag gtcgtggtct tcaagccgcc
    14941 cgccggcgcg gaccgcaacg agtgcggcgt accgggcttc gagccggcgt cgcgcaacca
    15001 gtcctgcccg cggcccaccc cgggccggtc cgatcagaac ttcatcaagc ggatcgtggc
    15061 cgtcgggggc gaccgggtga ggatcgagcg cgggcgcgtg atcctcaacg gcaagccggt
    15121 cgacgagccg ttcatccggc cgagcgagga ctgccagatc tgcaacctcc ccaaggagat
    15181 ccgcattccg ccaaagcact acttcatgat gggcgacaac cgtggggaga gcgcggacag
    15241 ccgcgagtgg ggcccggtgc cggaggatca gctcgtcggc caggccttct tcacgtactg
    15301 gccgccgaag cgcatcggca cgctctgagc gcggcaaagc cgcacgttcc gcacaaagcg
    15361 ctgcgaggtc gccggaccag cctctaggct cgcgcgcggc aagcagatga tggaccccgc
    15421 cgccgccact tcgcccgcag ccctggagga ggcgcccgag atgggcgcgt tgccggccac
    15481 ggcgccggcg ccgctcgccg aggccgccgc gccagcggag gtcccgccgg cccccgccga
    15541 ggccgccccc ccctcggttg ccgaggccgc cccgtccgcg gaggctccgc ccgcgcccgc
    15601 cgctc
//
LOCUS       DASVPL010000316         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_37894, whole genome shotgun sequence.
ACCESSION   DASVPL010000316 DASVPL010000000
VERSION     DASVPL010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37894"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VF517_18135"
     CDS             complement(<1..724)
                     /locus_tag="VF517_18135"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8054908.1"
                     /translation="MSPIRPTVQLVGRRLEPGHHTMRDMLTRAAQPHEWLEAGTPEAD
                     ALLARHGLADPELPVLIDGDEVHEGITPARLGEAWRMSAPPSKEHYDFAVIGAGPAGL
                     AAAVYAASDGLSTVVFERDLPGGQASHTSMIENFFGFPEGIGGAELARLAGRQAEGFG
                     AELVLLRGVEGGGPDGDRPRFQIAGGYEVTADVGLASPGMEWRRLEADGVDELIGRGV
                     YYGAGRSEAAQCGNDDVVVVGAG"
     gene            complement(773..1738)
                     /locus_tag="VF517_18140"
     CDS             complement(773..1738)
                     /locus_tag="VF517_18140"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8054909.1"
                     /translation="MAGARRFSLGSESSEDGSFTRQESRFRDWVSADGSTEFPAEAGR
                     YHLYVSWACPWAHRTIIGRRLKGLEDAIGLSVVDPIRDERGWAFSGGEYVDRVNGFRY
                     LSEAYDATDPEFVGRYSVPVLWDKVGGRIVNNESGDILRMLNSGFGELADDSVDLYPA
                     EHRAEIDQLGEHIYDTVNNAVYKAGFTTSQAVYEREVTTMFETLDQLDARLAGQRFLF
                     GDRPVETDWRLFTTLVRFDAVYFIHFKCSIRRLVDFENLWPYARDLYQQPGIAETVRF
                     DEIRRHYYETHPMINPSGLVALRPAGDFGAPHGRERLARRPVRAA"
     gene            1783..>2994
                     /locus_tag="VF517_18145"
     CDS             1783..>2994
                     /locus_tag="VF517_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054910.1"
                     /translation="MPTEGAVRRGIAAISIVASLALAAQAGAALPSVKSGPRPGPDAL
                     YAPPPTEVPQLENAAPWRAEPILVSGAQAYRDGEWLYQDYLHDDHGAAGARDPNDPFG
                     IDAHLFSPAAGSMTYPTDPVYAHNAADLVELRVRPGAGETLFRVTLNTLKDPARTAFT
                     LALGNSAGPRPWPHGAGVISPAEVFVTVHGANADAQNATTGGPVSGATASVDARRRQV
                     EVRVPHSAWSPGTGKARVSVGVGLWDPEAGAYLKPGGTEATATRPGGVAPSNAALFNV
                     GPRLAEPQPEVSQYGAGVTIGDAAAGAAVQGAWWREKAQADALRIGDVTPFAAEVDFG
                     KLAAGTDDDSGVPRTGPINRILASRHQFGQGIEPGRVCYDLAAGFSAGARCEGRFVGQ
                     LQPYALYVPAKP"
BASE COUNT          405 a         1142 c         1063 g          384 t
ORIGIN      
        1 tgcccgcgcc gacaaccacc acgtcgtcgt tgccgcactg ggcggcctcg ctgcgccccg
       61 cgccgtagta gacgccgcgc ccgatcagct cgtccacgcc gtccgcctcg agccgccgcc
      121 actccatgcc agggctggcc agccccacgt cggcggtcac ctcgtagccg ccggcgatct
      181 ggaagcgcgg gcggtcgccg tccggtccgc ctccctcgac gccgcgcagc agcacgagct
      241 ccgcgccgaa gccctcggcc tggcggccgg cgagccgcgc cagttccgcg ccgccgatgc
      301 cctccgggaa gccgaaaaag ttttcgatca tcgaggtgtg cgacgcctgc ccgcccggca
      361 ggtcgcgctc gaagaccacg gtcgagaggc cgtcggacgc cgcgtagacc gctgccgcca
      421 gcccggccgg cccggcgccg atcaccgcga agtcgtagtg ctccttcgac ggaggggccg
      481 acatccgcca cgcctcgccg agccgagccg gcgtgatccc ctcgtgcacc tcgtcgccgt
      541 cgatcagcac cggcagctcc gggtcggcga gcccgtggcg cgcgagcagc gcgtcggcct
      601 cgggcgtgcc ggcctcgagc cactcgtgcg gctgagccgc ccgcgtgagc atgtcgcgca
      661 tggtgtggtg gccgggctcg agccgccggc cgacgagctg cacggtgggg cggatcgggc
      721 tcacgggttg agcctaaccg gcgcgaccaa ggcggcccgg gggccgcgcc gcctacgccg
      781 cgcggaccgg gcgccgcgcc agccgctcgc gcccgtgcgg tgccccgaag tcgcccgcgg
      841 gccgcagcgc caccaacccg ctcgggttga tcatcgggtg cgtctcgtag tagtgccttc
      901 ggatctcgtc gaatcgcacc gtctcggcga tcccgggctg ctggtagagg tcgcgcgcgt
      961 acggccagag gttctcgaag tccaccagcc ggcggatgct gcacttgaag tggatgaagt
     1021 agaccgcgtc gaagcggacc agcgtcgtga acaggcgcca atcggtctcg accggccggt
     1081 cgccgaacag gaagcgctgc ccggcgaggc gcgcgtcgag ctggtcgagc gtctcgaaca
     1141 tggtcgtgac ctcgcgctcg tacaccgcct gcgaggtcgt gaagcccgcc ttgtagaccg
     1201 cgttgttgac ggtgtcgtag atgtgctcgc cgagctggtc gatctcggcg cggtgctcgg
     1261 ccgggtagag gtcgacggag tcgtcggcga gctcgccgaa gccggagttg agcatccgga
     1321 ggatgtcgcc ggactcgttg ttgacgatcc gcccgccgac cttgtcccac agcacgggca
     1381 cgctgtagcg gcccacgaac tcggggtccg tcgcgtcgta ggcctcgctc aggtagcgga
     1441 agccgttcac ccggtcgacg tactcgccgc cgctgaaggc ccagccgcgc tcgtcgcgga
     1501 tcgggtccac cacggacagg ccgatcgcgt cctcgagccc cttcagccgc cgcccgatga
     1561 tcgtccggtg ggcccacggg caagcccacg agacgtagag gtggtagcgg ccggcctcgg
     1621 cggggaactc ggtcgagccg tcggccgaca cccagtcgcg gaagcgactc tcctgacgcg
     1681 tgaaggaacc gtcctcggag gactccgatc cgagcgagaa cctgcgtgcg ccggccatgt
     1741 ccaaacgctg cccggagcgc tgcgcgcgca aacggtcgta ccatgcccac cgagggagca
     1801 gtgcgcaggg gaatcgccgc catctcgatc gtcgcgtcgc tcgcgctcgc agcgcaggcg
     1861 ggtgccgcgc tgccgtcggt gaagagcggc ccgcgccccg gccccgatgc cctgtacgcg
     1921 cccccgccga cggaggtgcc gcagctcgag aacgccgcgc cgtggcgcgc cgagccgatc
     1981 ctcgtctccg gcgcgcaggc ctaccgcgac ggcgagtggc tctaccagga ctacctgcac
     2041 gacgaccacg gcgccgccgg agcgcgcgat ccgaacgacc cgttcggcat cgacgcccac
     2101 ctgttctcgc cggccgcggg ctcgatgacc tacccgaccg accccgtcta cgcccacaac
     2161 gccgccgacc tggtcgagct gcgggtcagg cccggcgccg gggagacgct cttccgggtc
     2221 acgctgaaca cgctgaagga cccggcgcgc acggcgttca cgttggcgct gggcaactcc
     2281 gccggcccgc ggccgtggcc gcacggggcg ggcgtgatct cgcccgcgga ggttttcgtg
     2341 accgtccacg gcgcgaacgc cgacgcccag aacgcgacca cgggtgggcc cgtgagcggc
     2401 gccacggcct ccgtcgacgc gcgccgcagg caggtcgagg tccgcgtgcc gcactccgcc
     2461 tggagtcccg gcaccggcaa ggcgcgcgtc tcggtcggcg tggggctctg ggatcccgag
     2521 gccggcgcct acctcaagcc cggagggacc gaggcgaccg cgacccgccc cgggggcgtc
     2581 gcgccctcga acgccgcgct cttcaacgtc gggccgcgtc tcgccgagcc ccaaccggag
     2641 gtcagccagt acggcgcggg cgtgacgatc ggcgacgccg cggccggcgc cgccgtgcag
     2701 ggcgcctggt ggcgcgagaa ggcgcaggcc gacgcgctgc ggatcggcga cgtcacgccg
     2761 ttcgccgccg aggtcgactt cgggaagctc gccgccggca ccgacgacga ctccggcgtg
     2821 ccgaggaccg gcccgatcaa ccgcatcctc gccagccgcc accagttcgg ccaggggatc
     2881 gagccgggcc gcgtctgcta cgacctcgcc gccggcttct ccgccggggc caggtgcgag
     2941 ggccgcttcg tcggccagct tcagccgtac gcgctctacg tgcccgccaa gccg
//
LOCUS       DASVPL010000317        22815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_77836, whole genome shotgun sequence.
ACCESSION   DASVPL010000317 DASVPL010000000
VERSION     DASVPL010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22815
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77836"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VF517_18150"
     CDS             complement(<1..647)
                     /locus_tag="VF517_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054911.1"
                     /translation="MELVQEIGSYAGFAAVVGLAILSALYFSQARDVKRLREWAGRAP
                     ERAAELEEGSRAQAAVPVAARPQPQVVRGEGAGTAVRPAPGGAAGGAAAGGASGVGGA
                     AAGGAAAAGGAAAGGAAAGAAAPAGAAAASAGASPATASAGAPALAGADTADDDDLDA
                     AEPVSAGAVGAATAAGSAANGSEEAADADAGAQPVGTPAAATPAGAVASAAGTAER"
     gene            complement(701..2617)
                     /locus_tag="VF517_18155"
     CDS             complement(701..2617)
                     /locus_tag="VF517_18155"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX8054912.1"
                     /translation="MRTRILDSVALRRTAQVAIDACLVALAYCMAYVLRFDPGIPGRY
                     EDLLAESVALVVLGKLLVFALFGLYHKLWRFVDQQDFETILKAVVVSSMVLVGVFFLI
                     PTSAATDPPRGVIALDFLLTLGLVGGVRFVVRAVIERPYRGAVARRGAREVLIVGAGN
                     GGQLVAAELRRNPELGIPIGFVDDDPRKQGMRIAGVKVEGTTEELDRVLDAAEPGEVI
                     IAIPSAPGVVRQKVVTACRSRGIPVRTLPTTFELLSGGVELMRQVREVKVEDVLGREP
                     VRMEIDRVGGYLAGEVVLVTGAGGSIGSELCRQIARVGPSRLVLVDNAENSLFEIRRE
                     LEHDRHFTRAIAILADCKDAVRMEEIFGEQRPAVVFHAAAYKHVPLMEENPVEAVRNN
                     AVATRIVAAAAGGAGARRFVLVSTDKAVTPATVMGASKALAEWAVEAAQHRFPHTRFL
                     TVRFGNVLGSSGSVVPIFRRQIAAGGPVTVTDPNMTRYFMTIPEAVQLIIRSGDVGRG
                     GEVFVLEMGDPVKIVDLARNMIRLSGREPDTEIAIDIVGRRPGEKIHEELFNPDERPQ
                     PTPAEKIVAATRPALEPDWVEGAFSRVEELVYSGDAHGLAAMVTGLAEERAASASWNA
                     TADESTIPAPERAS"
     gene            2676..3338
                     /locus_tag="VF517_18160"
     CDS             2676..3338
                     /locus_tag="VF517_18160"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX8054913.1"
                     /translation="MLVLASLTDPLVEFATNVVEEIGLAGVFVLMALESACIPIPSEA
                     TMLFAGFNVSEGEYTLFAATAVGSLANLAGSWVAYWVGRAGRVDILEKHGRKLHIKKS
                     HLEWADRWFERHGDATVFFTRMLPIIRTFISLPAGVARMPFWRFSVLTLAGCIPWVLL
                     LTFIGKEAGKNWEDWKDSLHYVDYFVAACIVVGVVYVLMRRRGGGGGAESEASDPATE
                     PA"
     gene            3365..4129
                     /locus_tag="VF517_18165"
     CDS             3365..4129
                     /locus_tag="VF517_18165"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX8054914.1"
                     /translation="MGAVQGPAELLPVSSSGHLVLVPALLGWSYGELDPEVRKAFEVA
                     LHAGTAAALLIGLRREVAEAVRELDPGRVLHHALGFAPPALVAATLERTIERRLSAPR
                     TAALAQVVAGAALGLADRAPAERARDDARPLDALAIGVAQAAALSPGVSRNGATLTAT
                     RLLRFERMAANRLSRHAALPIILAAAGLKGVRLAQRGLDRELAAPFAIGALASFASTL
                     LATRLVRTVDEAPSYAPFAIYRVGLGTAALAILRRT"
     gene            4139..5230
                     /gene="purM"
                     /locus_tag="VF517_18170"
     CDS             4139..5230
                     /gene="purM"
                     /locus_tag="VF517_18170"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX8054915.1"
                     /translation="MSESAYAAAGVDTSRAGSAVAGLVAVLRGIDPGRPSRAVLGSGH
                     YANVLRIDERTGIALSTDGVGSKVIVAEQLGRFDTVGIDCIAMNVNDVICVGAEPLAV
                     LDYIAVEDADPDMLRAIAEGLKRGAEDAGVEIPGGELAQLPELIRGHPSPRGFDLVGA
                     CFGTIALDSIVTGASIEPGDAIVGLPASGVHSNGLTLARSALTDLDERPPELGGASVG
                     ETLLEPTVIYVRAVLDLLRSDDVDVRGLAHITSGGMLNLLRLEAEVGYSIDSPLPRLP
                     VFELIAARSGAEEAELQEVFNMGCGFCVVVPAEQADAATAIAAARHPGAARIGTVTAN
                     GGVVELPSLGLVGRDGAGFAPTASSSGRR"
     gene            5276..5605
                     /locus_tag="VF517_18175"
     CDS             5276..5605
                     /locus_tag="VF517_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054916.1"
                     /translation="MDEHDLALDASLGEAVDFAQEQAADLATRAAALDSLARWLCAHA
                     ADLRDAQDPDGAVPPFLPFVVHEVVASAAEARAAAGRLHAVAPPEGSQAAQGLRGLTL
                     RTPDDAG"
     gene            complement(5617..5799)
                     /locus_tag="VF517_18180"
     CDS             complement(5617..5799)
                     /locus_tag="VF517_18180"
                     /inference="COORDINATES: protein motif:HMM:NF018946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054917.1"
                     /translation="MAEPVPAGSDVSAGTYSCTSCGNTIDVGSTQSLPPCPNCGNGSW
                     NTQSGGDSAKDPYPDR"
     gene            complement(5853..6671)
                     /locus_tag="VF517_18185"
     CDS             complement(5853..6671)
                     /locus_tag="VF517_18185"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8054918.1"
                     /translation="MPVQVRYFTDPGCPWSWAAEPSVRRLMVEFGDSLSWSWLMVGLS
                     REITADTKGLALHWLGVAASSGMPIDPLLWKDSPIRSTYPACMAVKAAAEQGEDAATR
                     YLRVLREGLMCRRRKLDTTEALVEAARFAGLDVERMRVDLGSHAIVEAFGADMEAARA
                     VPDEARAADKVAQGEGGDRVSIPCAEIEGQWWFGGSYDELRAAAIAAGAEPTGAERPS
                     IEDALRRFGRMATLEVQEVCGLPGPRAHAELWRLASEWQVKPVSALTGYLWEHA"
     gene            6692..8185
                     /locus_tag="VF517_18190"
     CDS             6692..8185
                     /locus_tag="VF517_18190"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8054919.1"
                     /translation="MIGTLINGRFRLDEQVGAGGMSTVYRAFDETLQRTVAIKLMHRS
                     LSGDPDQLERFRREARAAARLSHPHVVTVIDAGEDDGHPYIVFEFVAGETLKGRIKRL
                     GRLPVAEAVAYGIEIGRALAAAHAERLVHRDVKPQNVLIDPDGRAKVTDFGIARSLED
                     GGLTATGRVLGTTDYVSPEQALGEDVTAESDVYALGIVLYEMLTGELPFKAESQVGVA
                     MKHVQDPMPDVQLRRPEVSASLAAVIDRATSKQVENRYGSADDMVWELEQALAIEVAR
                     AGEATGEATTVLRALPDETGEMAPERLRHPRRVLALSVVVIALLAGAAVFALTNARRG
                     DPEEPAVAAAPPPAPVAIRAAHDHDPYGTDRSEHPEQARFAIDADNESVWSTEEYESG
                     QLAPKDGVGIYLDTGTPVAARRLELRTPTPGFEVQVYGSNQAPFAEPGPETFQHWGEP
                     LAQKQVDAKRSIAIDTGGRALRYYLVWIVKLPPSGGKAEIATLRLLR"
     gene            complement(8189..10027)
                     /locus_tag="VF517_18195"
     CDS             complement(8189..10027)
                     /locus_tag="VF517_18195"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX8054920.1"
                     /translation="MAEVVEGALVDDRYEVGRRLGSGGMADVYLAHDRQLGRDIALKV
                     LHRRFARDGQFVERFKREAQSAAGLQHPHVVGIYDRGEHDGTYYIAMENLPGRTLRQV
                     VNEEAPLDQERTIAYGLQILDAAGFAHRNGIVHRDFKPHNVICGPDGQLKVTDFGIAR
                     AGASEMTETGSIMGTAQYLSPEQAQGQPVDAASDLYSIGVMLYEMLVGRVPFDGDTAV
                     AIALRHVSDAPAPIRELRPDVHPALEAAIMRALQKDPAARYASAEEFAAALGMARDAI
                     HSGDPGQDTAAWGALIAPTELTEEQDGRRRRWPWVALVLLLLLGAAALAFALGGDDRV
                     EVPNLVGKPAPQAVTELARLGLKSDVKPVESEAPEESVVSHDPAAGEEVEEGATVTLR
                     VSSGPGTKIVPEVRGKTEKQAAKLLADAGFEYTRQDEASASIPAGSAIRTDPAGGTEQ
                     RTGARVQLFISTGPKRVDVPDVVGLEVDDARAELSDRGLRAVVQRVESDEPEGQVTGQ
                     NPGEGTEVNEGTRVTISVSKGTDEVRVPNVEGDEAEAARAELEREGFKVVDHDEPGPP
                     EDEGKVVRQQPGGGRAPEGSTVTIWVGVPEEEEEPPPEDTDAEPSP"
     gene            complement(10020..11456)
                     /locus_tag="VF517_18200"
     CDS             complement(10020..11456)
                     /locus_tag="VF517_18200"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX8054921.1"
                     /translation="MNKPLRRLFVVFVLMFGVLVGFTSYWTVFGAEKLEEKPSNRRPI
                     LKAQKIPRGLILASDGTKLATNRRTGNRETKRYYRVYPQSRLFSHAIGYSFVSRGSAG
                     IERSYNDELIGEHDELESVIDQLGGGVKEGDDIRTHLDAQAQRVAIQQLAGRRGSIVA
                     LDPDTGAVKVMVSIPDFDPNSIPQQFSQLNRDASSPLFNRATQARYPPGSTFKVVTAA
                     AALDSGRYNPGSLVDGRNNKPISGVPLANSGGADFGTINLTDALTQSVNTVWGEVGEK
                     LGKGTMLKYMERFGFLSEPPLDYPKAQITPSGIYSGQRLLDEDDDIDIGRVAIGQERL
                     QVTPLQMAMVAATVGNGGILMKPRIVDRVVEPDGSVRQRVRPSRQGRVISRKAADELR
                     GMMSKVVEEGTGTASALAGIRSGGKTGTAEVQNGASNQAWFIAFAPLDDPEVAIAVTV
                     ERTQGQGGTIAAPIAKQVMQSLLGGDGG"
     gene            complement(11453..12763)
                     /locus_tag="VF517_18205"
     CDS             complement(11453..12763)
                     /locus_tag="VF517_18205"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX8054922.1"
                     /translation="MSARTRELFALVPVSLLVAAGFAAILITRQDAGYQINELTATYG
                     GVFLAACVLGHLFIRARLPDADPYLFPLVALLAAFGLVEIYRISPTRAGQQAIWFGVG
                     LALFCVTILFLRDHRVLERYRYLIALGGIGFLMLPRVPGIGQQTNGAYLSVGAGPVQF
                     QPAEFAKLAIIVFLASYLSETRDVLVRGRLPRLSMKHLGPLLVIWGLAMLMLIVIRDL
                     GSSLMFFGGFLALVYVATSRISLVVGGLGMFLGGAWFLSQRISHVQDRVDVWIDPFKP
                     ELVEKEGYQISQGLYAQADGGLFGEGLGQSLIQQSDGTPILPAAHTDLIYAVITSELG
                     LFGAVGVLLVALMIVARAFKTAVLATDGFSKLLAAGLGAVFALQVFVIVGGVVKAIPL
                     TGVTMPFVSYGGSSIVANFVLIALLMIVSDKARRDPAAQRGGLV"
     gene            complement(12763..14088)
                     /locus_tag="VF517_18210"
     CDS             complement(12763..14088)
                     /locus_tag="VF517_18210"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX8054923.1"
                     /translation="MSLRIVEKASLTDVGRQRQSNEDAFFERAPLFAVADGMGGARAG
                     EVASRMAVEELAEMEDPGARAEESLRTVARSANRRIYDMAQSDSEHAGMGTTFTAVLV
                     SGREVAVGHVGDSRLYRLRDGAFERLTDDHSLVEELVRQGKLTPQEAEVHPQRSIITR
                     ALGPESDVKVDTFTHTARDGDVYLLCSDGLSGMASDDQMAEILRGSRSLEDAAQGLID
                     AANAAGGKDNITAVLFRVAEDEGAEPESDTLGDGATQAGVTTAAVREAVAEDETRRGA
                     SVADPREAGTMTLSAEEAARARAQAGGAARTEAGARAGPARDRPAALTHGDDSSRPPR
                     RPARPRLGGGTALFLLALALVAVGLYAGSRQVYFIGADDRGAVSLFRGLPYELPLGVD
                     LYTHEYSSSVPVSAVEDRRQRSRLLNHELRGRDDAEERIRELERAETPR"
     gene            complement(14085..14564)
                     /locus_tag="VF517_18215"
     CDS             complement(14085..14564)
                     /locus_tag="VF517_18215"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8054924.1"
                     /translation="MTDPIAVLLKFGFLAVLYLFLLWVVRSALKDMAGAGRMPDEDDE
                     GAASGRRDRGSAMDLRSGVRPRLEVVAALGYEPGTAFEIGDSATLGRSPTADVQIDDP
                     YASSAHARIFPRGQFMYVEDMGSTNGTFLNGRQLRKPEQLRVADTVRIGDTEYRYQE"
     gene            complement(14584..15354)
                     /locus_tag="VF517_18220"
     CDS             complement(14584..15354)
                     /locus_tag="VF517_18220"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX8054925.1"
                     /translation="MSVLRNLEAKLEGLVGGAFSRAFRSKVEPVEIARKLAKEMQDNQ
                     TRSISHTYVPNLYTVWLSPEDRGHFEGYEDGLKKELSDYLLEHARTEGLALVTRPTIE
                     FETDDRLSLGEFGIQAQLIQAPEEEAPDWPADAGAAAGDFGHTMVYSPASSPRKLAPR
                     DVPRGGRALFIGEDKRAVLSGSRLVIGRSRDCDITVEDANVSRRHAELRNEDGRWVVA
                     DLGSTNGVKVNGRRVEHAALEPGDEVVFGLARFRFELE"
     gene            complement(15367..17229)
                     /locus_tag="VF517_18225"
     CDS             complement(15367..17229)
                     /locus_tag="VF517_18225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8054926.1"
                     /translation="MTTEQLGPPDERTRVVGEAPASPPRRFEPAIEATEPLSGGGRRA
                     PTDRLVLGRYRLERKLGAGGFGVVWLAFDEKLEREVAVKVIQADGDDPVTHRAAREAR
                     VAARLNHPGIVSLYELGEDDEAVYLVSELVPGRTLAELVRAGAVSDRDVARLGVALCD
                     ALAHAHDRKVVHRDVKPQNVIVVAQPAAGAGFAKLADFGVAQLAVGDPLTRTGDVVGT
                     LAYMSPEQAEGARVSAAADVYSLALTLYEAWAGVNPVRGPTPAATARRLGRRLPLLSA
                     YRPDLPPDMCAWIDAALDPVAERRPELGALRDALVREADHLGDSGGLAAPETLDRVGI
                     PRTEVLPAAVALPSERTLDLVTRALAGTAAAALVLAGIETLGPDPTFPVAAWAAATAV
                     AVALLPRLGWLAAAIGVTGWLGSELLDGTALVVGLTLAAVPVLLLRGGRLWSAPVAAP
                     LLGVIGIGPAFVGAAGLASTAWRRAGLGAAGFLWLAAAELLSSDELLFGPPQDASSRG
                     EWAGSLSGAMTDAIGPLVLSPLILVALAWGAFAAALPLVVRGRSLPLDAIGALVWAAA
                     LVAAHRGIAELAAGDVPLDEARGAAAGAAIGAAVALATVWSGLRPRPGDSPPVP"
     gene            17462..18463
                     /locus_tag="VF517_18230"
     CDS             17462..18463
                     /locus_tag="VF517_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX8054927.1"
                     /translation="MVRRAIAAAGGLVLIILFFLFVKSCRENAREDAFRNYGRDVAAL
                     VQESDQASQQLFGLLTRPGRRSPLELQQAVNGYHSDAAQLVDRARDTDHPDELDTAQR
                     WFVESLQFRRDGIERIARELPTALGDQGREEATARITVTMRSFLTSDVLYSDRFIPHL
                     TNEVKKQKLTGEVNIPRSRFLPDLQWLRPTVVAERIDAIRGGGGGTGTAAPGLHGTAL
                     VSVTAKPGGKALVPGGATDIAVSPRLSFDVAVSNGGEHEERDVTVSLSIAGAGKPLVR
                     DQRLPSIKPGEQKTVSIPLAATPTTGRPVTITVEVKPVPGEKKVDNNKQRYPAIFTR"
     gene            18486..18998
                     /locus_tag="VF517_18235"
     CDS             18486..18998
                     /locus_tag="VF517_18235"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX8054928.1"
                     /translation="MDDLSTTEGIAALAAGGVALLALILATVLAFKLRRMRQAQTAVL
                     GEYGSRDLVSHAERLESGFTQLRDWVEESMHSIDSRMGQVEGRVDGCIAYHSLIRYDA
                     YNELSGHQSSSMALLDSHRNGLVLSAIVHRDQARLYVKQVQGGEGEVALSPEEQEAVE
                     TALASPLRAA"
     gene            19004..19840
                     /gene="pheA"
                     /locus_tag="VF517_18240"
     CDS             19004..19840
                     /gene="pheA"
                     /locus_tag="VF517_18240"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX8054929.1"
                     /translation="MRVAFLGPAGTYTEEALRHSAPAGTEFEEVPYATVYETVMAVQS
                     GAAERAVVPIENSLEGSVTATLDALAGEADRVRILAEVVRPVSHCLVARGGVEVEAVE
                     RVLSHPQALAQCSELLRGRLAHAEPASAPSTADAVRTVAEGSEPWAAIGTRLAAEMYG
                     CEVLAEGVEDRSDNLTRFVWLAPEGTEPPHEPAKTSIVFWGFNDESPGALVGVLAELA
                     DRGINLTKIESRPRRVQLGHYMFFADLDGGVSEPPVRDALAALGERVQALKVLGSYPI
                     GG"
     gene            19854..20393
                     /locus_tag="VF517_18245"
     CDS             19854..20393
                     /locus_tag="VF517_18245"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8054930.1"
                     /translation="MAIASSTGSGSNSRVLVLNASYEPINVCTVRRAVVLVLKQRAEV
                     LERSDRDLHAESLTVARPAVIRLMTYVRVPRDAHSRKITRRAIFARDGWACQYCGAGH
                     GTLTVDHVIPRSKGGHSGWDNIVTCCAPCNRRKGDQLPKVVGMHPRRAPTPPSPTIFV
                     HVAVTRVPDTWQQYLPAAA"
     gene            complement(20390..21535)
                     /locus_tag="VF517_18250"
     CDS             complement(20390..21535)
                     /locus_tag="VF517_18250"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8054931.1"
                     /translation="MISPELTDFQDRIVGSWTRDSHRDAALRYVTGLLRTGPKRRSTL
                     RGIQEAIDGDRALYERLQGFLVDATWDPVVVMRGLARYAAESFDDIQAWMFTETVVPK
                     RGDKSPGVALQYSPASGRQMNCQRVISLHAVGRRRGRAVSLPVGWSMFLPHTWLSVPE
                     HLAQVPAEVHRTHFDSIRYGCWRSRLLERVRDALDRAGLSEASPWKSALHAPVVSDRL
                     YVDEVDECEDDLRRIEDVRGLSFVLPVPRHSVELSEFEEGTVVMQPFGANHETRLALR
                     WRIPHADGWYQPERARNRSLLAEWPLGVDRPSGYWLVGGPLRQSPSTPMWELGRCALI
                     PRNVDLGWEQLRELGIDDYEGRSLSGWHRHCALVSLAYAYKLEQQRG"
     gene            21669..>22815
                     /locus_tag="VF517_18255"
     CDS             21669..>22815
                     /locus_tag="VF517_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054932.1"
                     /translation="MRRVQVVGLLVCVLALAWAPAARAEQAIDDDPTVSGPSNTVETV
                     IGAGWEGDLLRTLAEGQGREEVNGAEWNAPLSGGTYEVSVWIPRGYGGTYVRYLIAHK
                     GGVSEVRLRQAAHEDVWVTLGTFEIDGPTATVRSTDAGGIAGQSMVWDAVRWTKVDPA
                     VSTPDPAADEHLVDDPVIIGPAEYVTTFVGVGYDGDLLRTYAEGSDHRQINGAEWRTP
                     LTSGRFRVQVYIPSEHGEGPVRYFVPHVGGEREILIDQSGYSNVWVDLGEFDLAGPEG
                     VVRSTDATGTRGNEVAWDAVRWVRVPSAPQAPATDPGGAPSSDQPGSDQPQTGPPATA
                     NSDGAADPDGDGVPNVLDACDGEHRGALDSNGDGCPGPTKVLARQTRR"
BASE COUNT         3111 a         8388 c         8245 g         3071 t
ORIGIN      
        1 cgctccgcgg tgcccgccgc ggaggcgacc gcgccggcgg gggtggcggc cgcgggcgtg
       61 ccgacgggct gcgcgccggc gtccgcgtcg gcggcctcct cggatccgtt cgcggccgac
      121 ccggcggcgg tggcggcccc caccgcgccg gcggagacgg gctcggccgc atcgagatcg
      181 tcgtcatcgg cggtgtcggc tccggcgagc gcgggcgcgc cggccgatgc cgtcgccggg
      241 ctcgcgccgg ccgacgcggc ggcggctcct gccggggcgg cggctcccgc tgcagctcca
      301 ccggccgcgg caccgccggc cgctgcagct ccaccggccg cggcgccgcc gaccccggag
      361 gctccgcccg ccgcggctcc gcccgcggca ccacccggcg cgggtcgaac cgcggtccct
      421 gccccctccc cgcgtaccac ctgcggctgc gggcgcgccg ccaccggcac ggcggcctgg
      481 gcgcggctcc cctcctccag ctcggcggcg cgctcggggg cgcgaccggc ccactcgcgc
      541 agccgcttaa cgtcgcgcgc ctgcgagaag tagagcgccg acaggatcgc gaggccgacc
      601 acggccgcga atcccgcgta ggaaccgatc tcctgaacga gctccactcg ggctggacct
      661 taccccggca cgcggccgct acgcgggcgc gagggccgca ctacgacgca cgctcggggg
      721 ccgggatcgt gctctcgtcg gcggtcgcgt tccacgacgc ggaggccgcg cgctcctcgg
      781 cgaggcccgt gaccatcgcc gcgagcccgt gcgcgtcgcc cgagtagacc agctcctcga
      841 cgcgcgagaa cgccccttcg acccagtccg gctccagcgc gggccgggtc gcggccacga
      901 tcttctcggc gggagtgggc tgcgggcgct cgtccgggtt gaacagctcc tcgtggatct
      961 tctcgccggg ccggcgcccg acgatgtcga tcgcaatctc cgtgtccggc tcgcgacccg
     1021 acaggcggat catgttgcgc gcgaggtcga cgatcttcac cgggtcgccc atctcgagca
     1081 cgaagacctc gccgccgcgg ccgacgtcac ccgagcggat gatgagctgg accgcctccg
     1141 ggatcgtcat gaagtagcgc gtcatgttcg ggtcggtgac cgtcaccggg ccgccggcgg
     1201 cgatctggcg gcggaagatc ggcaccacag atccggacga gccgagcacg ttgccgaagc
     1261 gcacggtcag gaagcgcgtg tgcgggaagc gatgctgcgc ggcctcgacc gcccactccg
     1321 ccagcgcctt cgacgcgccc atgaccgtcg ccggggtgac cgccttgtcg gtcgagacga
     1381 gcacgaagcg gcgcgcgccg gccccgcccg ccgcggcggc gacgatccgc gtggccaccg
     1441 cgttgttgcg cacggcctcg accgggttct cctccatcag cggcacgtgc ttgtaggcgg
     1501 cggcgtggaa gacgaccgcg gggcgctgct cgccgaagat ctcctccatc cggaccgcgt
     1561 ccttgcagtc ggcgaggatc gcgatcgcgc gggtgaagtg gcggtcgtgc tcgagctcgc
     1621 ggcggatctc gaacaggctg ttctcggcgt tgtccacgag cacgagccgc gacgggccca
     1681 cgcgcgcgat ctggcggcac agctccgatc cgatcgaacc gccggcgccg gtcaccagca
     1741 ccacctcgcc ggccaggtag ccgccgacgc ggtcgatctc catccgcacg ggctcgcggc
     1801 cgagcacgtc ctcgaccttc acctcgcgca cctggcgcat cagctcgacg ccgccggaga
     1861 gcagctcgaa cgttgtcggc agcgtgcgca cggggattcc gcgcgagcgg cacgcggtga
     1921 ccaccttctg ccgcacgact cccggcgccg aggggatcgc gatgatcacc tcgccgggct
     1981 cggcggcgtc gagcacccgg tcgagctcct cggtcgtgcc ctcgaccttc acgcccgcga
     2041 tccgcatgcc ctgcttgcgc gggtcgtcgt cgacgaaccc gatcgggatg ccgagctcgg
     2101 ggttgcgccg cagctcggcc gcaacgagct ggccgccgtt gccggcgccg acgatcagca
     2161 cctcgcgcgc gccgcgcctg gccaccgcgc cgcggtaggg ccgctcgatc acggcacgca
     2221 ccacgaaccg cacgccgccg acgaggccga gcgtgagcag gaagtcgagc gcgatcacgc
     2281 cgcgcggggg gtcggtggcg gccgaggtcg ggatcaggaa gaagaccccc accaggacca
     2341 tgctcgacac gacgaccgcc ttgaggatcg tctcgaagtc ctgctggtcc acgaagcgcc
     2401 agagcttgtg gtagagcccg aacagcgcga acacgagcag cttgccgagc accacgaggg
     2461 cgaccgactc ggcgagcagg tcctcgtagc gacccgggat gccgggatcg aagcgcaaca
     2521 cgtacgccat gcagtaggcg agcgccacca ggcaggcgtc gatcgccacc tgcgcggtac
     2581 ggcgcagcgc cacggagtcg aggatgcgag ttctcactct cggagggtca ccgtggagat
     2641 cccagtctag gccgtcgcta gcctaacccg ccgccatgct ggtactcgcc tcattgaccg
     2701 atccgctggt cgagttcgcc accaacgtgg tcgaggagat cgggctcgcc ggggtgttcg
     2761 tgctgatggc gctcgagagc gcctgcatcc cgatcccgag cgaggcgacc atgttgttcg
     2821 cggggttcaa cgtgagcgag ggcgagtaca ccctcttcgc cgcgacggcg gtcggatcct
     2881 tggccaacct ggccggctcg tgggtggcgt actgggtcgg ccgcgcgggt cgcgtggaca
     2941 tcctcgagaa gcacggccgg aagctccaca tcaagaagag ccacctcgag tgggccgacc
     3001 gctggttcga gcgccacggc gacgccaccg tgttcttcac ccggatgctg ccgatcatcc
     3061 gcaccttcat ctcgctgccc gccggggtcg cgcggatgcc gttctggcgc ttcagcgtgc
     3121 tcacgctcgc cgggtgcatc ccgtgggtcc tgctcctgac cttcatcggc aaggaggcgg
     3181 gcaagaactg ggaggactgg aaggactccc tgcactacgt cgactacttc gtggccgcgt
     3241 gcatcgtcgt cggggtggtc tacgtgctga tgcggcgccg cggcgggggc ggcggcgctg
     3301 agtccgaggc gagcgacccc gcgacggagc cggcgtaggc ctctcggccg ggcaggccgc
     3361 cgcactgggt gccgtgcagg ggcccgcgga gctgctcccg gtgtcgagct cgggccacct
     3421 cgtgctggtc ccggcgctgc tcggctggag ctacggcgag ctcgatcccg aggtgcgcaa
     3481 ggcgttcgag gtggcgctgc acgccgggac cgccgcagcg ctgctgatcg ggctgcggcg
     3541 ggaggtcgcg gaggcggtgc gcgagctcga cccgggccgc gtgctccacc acgcgctcgg
     3601 gttcgcgccg ccggcgctcg tggcggccac gctggagcgg acgatcgagc ggcggctgag
     3661 cgcgccgcgc acggcggcgc tggcccaggt cgtggccggg gccgcgctgg gactcgccga
     3721 ccgcgcgccc gccgagcgcg cccgcgacga cgcgcggccg ctcgacgcgc tggcgatcgg
     3781 cgtggcgcag gcggccgcgc tctcgcccgg cgtctcgcgc aacggcgcca cgctcacggc
     3841 cacccggctg ctgcgcttcg agcgcatggc cgcgaaccgc ctctcgcgcc acgcggccct
     3901 cccgatcatc ctcgccgccg cggggctcaa gggcgtccgg ctggctcagc gcggactgga
     3961 ccgcgagctg gcggcgccgt tcgcgatcgg cgcgctcgcg tcgttcgcct cgacgctcct
     4021 cgccacgcgg ctcgtgcgga cggtcgacga ggccccgtcc tacgcgccgt tcgcgatcta
     4081 ccgggtcggc ctcggcaccg cggcgcttgc gatcctgcgc cggacgtaga gtgaacccgt
     4141 gagcgagagc gcctacgcgg ccgccggggt cgacacctcg cgtgcgggga gcgccgttgc
     4201 cgggctcgtg gccgtgctgc gcgggatcga cccggggcgg ccgtcgcgcg cggtgctcgg
     4261 ctcggggcac tacgccaacg tgctgcggat cgacgagcgc acggggatcg cgctctcgac
     4321 cgacggcgtc gggtcgaagg tgatcgtcgc cgagcagctc gggcgcttcg acaccgtcgg
     4381 catcgactgc atcgccatga acgtgaacga cgtgatctgc gtcggggccg agccgctcgc
     4441 cgtgctcgac tacatcgcgg tcgaggacgc cgacccggac atgctgcgcg cgatcgccga
     4501 gggcctcaag cgtggcgccg aggacgcggg cgtggagatc cccggcggcg agctggcgca
     4561 gctcccggag ctgatccgcg ggcacccgtc gccgcgcggg ttcgacctcg tcggcgcgtg
     4621 cttcggcacg atcgccctcg actcgatcgt caccggcgcg agcatcgagc ccggtgacgc
     4681 gatcgtcggg ctccccgcga gcggcgtcca ttcgaatggc ctcacgctgg cgcgcagcgc
     4741 actgaccgac ctcgacgagc gcccgccgga gctcggcggc gcgagcgtcg gcgagacgct
     4801 gctcgagccg accgtgatct acgtgcgcgc ggtgctcgac ctgctgcgct ccgacgacgt
     4861 cgacgtgcgc gggctcgcgc acatcacgag cgggggcatg ctgaacctgc tgcgcctgga
     4921 ggccgaggtg ggctactcga tcgacagccc gctgccgcgg ctgccggtgt tcgagctgat
     4981 cgccgcgcgc tcgggcgccg aggaggccga gctccaggag gtcttcaaca tgggctgcgg
     5041 cttctgcgtg gtggtcccgg ccgagcaggc cgacgcggcg accgcgatcg ccgccgcgcg
     5101 ccaccccggc gccgcgcgga tcggcacggt caccgcgaac ggcggcgtgg tcgagctgcc
     5161 gtcgctcggg ctcgtcggcc gcgacggcgc gggcttcgcg cccaccgcct cgtcgtcggg
     5221 ccgccgctag agcgcgttga tcggctcctc cagggtgcag cgtagccttc gccctgtgga
     5281 cgagcacgat ctcgcgctgg acgcgagcct cggcgaggct gtcgatttcg cacaggagca
     5341 ggcggcggac ctggcaacgc gagccgcagc gcttgactca ctcgcccgct ggctgtgcgc
     5401 gcatgctgct gacctgcgtg acgcccaaga cccggacggt gccgtcccgc cctttctccc
     5461 gttcgtggtg cacgaggtgg tggcgtcggc ggctgaggcg cgcgcggcgg cgggcaggct
     5521 gcacgccgtt gcgccgcctg aggggagcca ggcggcacag ggtctgcggg gactgaccct
     5581 gcgcaccccg gacgacgccg ggtaggccaa cggcgcctag cggtccgggt acggatcctt
     5641 cgcgctgtcg ccgcccgact gggtgttcca cgaaccgttg ccgcagttcg ggcacggggg
     5701 cagcgactgc gtcgagccca cgtcgatcgt gttaccgcag ctcgtgcagg agtacgtgcc
     5761 cgccgagacg tcgcttcctg ccgggaccgg ttcagccatg acgctctcct aatccggggt
     5821 ggtagatggg cgctacccgg cgaccgagcc gcctaagcgt gttcccacag gtacccggtc
     5881 agcgcggaga ccggcttgac ctgccactcc gacgccagcc gccacagctc ggcgtgcgcg
     5941 cgcgggccgg gcaggccgca tacctcctgg acctcgagcg tggccatgcg gccgaagcgg
     6001 cgcagggcgt cctcgatcga cgggcgctcc gcgcccgtgg gctccgctcc cgccgcgatc
     6061 gcggccgcgc gcagctcgtc gtaggagccg ccgaaccacc actgcccctc gatctcggcg
     6121 cacgggattg agacgcggtc tccgccctcg ccctgcgcga ccttgtccgc cgcgcgcgcc
     6181 tcgtcgggca cggcgcgcgc cgcctccatg tcggccccga acgcctccac gatcgcgtgc
     6241 gacccgaggt ccacgcgcat gcgctccacg tcgagccccg cgaagcgggc cgcctcgacc
     6301 agcgcctcgg tggtgtcgag cttgcggcgc cggcacatca gcccctcgcg cagcacgcgc
     6361 aggtaccgcg ttgcggcgtc ctcgccctgc tccgcggccg ccttcacggc catgcaggcc
     6421 gggtaggtcg atcggatggg cgagtccttc cagagcagcg ggtcgattgg catccccgac
     6481 gaggcggcca cgcccagcca gtgcagcgcg agccccttcg tatcggctgt tatctctcgc
     6541 gagagcccga ccatgagcca ggaccaggac agggagtcgc cgaactccac catcagacgc
     6601 cgtaccgacg gctcggccgc ccaggaccac gggcagcccg ggtcggtgaa ataacgcact
     6661 tggaccggca cgccccgcaa aataacgaca gatgatcggg acgctcatca acggccgctt
     6721 ccggctcgac gagcaggtcg gcgccggcgg catgtcgacg gtctaccgcg cgttcgacga
     6781 gacgctccag cgcacggtcg cgatcaagct catgcaccgc tcgctgtcgg gcgacccgga
     6841 ccagctcgag cgcttccgcc gcgaggcgcg tgcggccgcc cggctctcgc atccgcacgt
     6901 cgtgaccgtg atcgacgccg gcgaggacga cggccacccg tacatcgtct tcgagttcgt
     6961 ggcgggcgag accctgaagg gccggatcaa gcgtctcggg cggctgccgg tggccgaggc
     7021 ggtggcctat gggatcgaga tcggccgcgc gctcgccgcc gcccacgcgg agcggctcgt
     7081 gcaccgcgac gtgaagccgc agaacgtcct gatcgacccc gacggacgcg ccaaggtgac
     7141 cgacttcggc atcgcgcgct cgctcgagga cggcgggctc accgcgaccg gtcgcgtgct
     7201 cggcacgacc gactacgtgt cgccggagca ggcgctcggc gaggacgtga ccgccgagtc
     7261 cgacgtctac gcgctcggga tcgtgctcta cgagatgctc accggcgagc tgccgttcaa
     7321 ggcggagagc caggtgggag tcgcgatgaa gcacgtgcag gacccgatgc cggacgtgca
     7381 gctccgccgg cccgaggtgt ccgcgtcgct ggcggccgtg atcgaccggg ccacgtcgaa
     7441 gcaggtcgag aaccgctacg ggagcgccga cgacatggtg tgggagctcg agcaggcgct
     7501 cgccatcgag gtcgcgcggg cgggcgaggc caccggcgag gccaccaccg tgctgcgcgc
     7561 gcttcccgac gagaccggcg agatggcgcc cgagcggctg cgccacccac gccgcgtgct
     7621 cgcgctgtcg gtggtcgtga tcgcgctgct cgccggggcg gcggtgttcg cgctcacgaa
     7681 cgcgcggcgc ggggatcccg aggagccggc cgtggccgcc gctcctccgc cggcgcccgt
     7741 cgcgatccgg gccgcgcacg accacgatcc atacggcacc gaccgcagcg agcatcccga
     7801 gcaggcgcgc ttcgcgatcg acgccgacaa cgagagcgtg tggtccaccg aggagtacga
     7861 gagcggccag ctcgcgccga aggacggcgt cgggatctac ctcgacacgg gcacgccggt
     7921 ggcggcgcgg cggctcgaac tgcgcacgcc cacgcccggc ttcgaggtgc aggtgtacgg
     7981 ctcgaaccag gcgccgttcg ccgagccggg gccggagacg ttccagcact ggggcgagcc
     8041 gctggctcag aagcaggtgg acgccaagcg cagcatcgcg atcgacaccg gcgggcgtgc
     8101 gctgcgctac tacctcgtct ggatcgtgaa gctgccgccg agcggcggca aggcggagat
     8161 cgccacgctc cgcctgctgc gctgagccct agggcgaggg ctccgcgtcc gtgtcctccg
     8221 gcggcggctc ctcttcctcc tcgggcacgc ccacccagat cgtcacggtc gagccctccg
     8281 gcgcgcgccc gccgcccggc tgctggcgca ccaccttccc ctcgtcctcc ggcggcccgg
     8341 gctcgtcgtg gtcgaccacc ttgaacccct cgcgctcgag ctcggcgcgc gcggcctcgg
     8401 cctcgtcacc ctcgacgttc ggcacccgca cctcgtcggt gcccttcgac accgagatcg
     8461 tgacccgcgt gccctcgttc acctcggtgc cctcacccgg gttctggccg gtcacctggc
     8521 cctccggctc gtccgactcg acgcgttgca ccaccgcccg caggcccctg tcggacagct
     8581 cggcgcgcgc gtcgtcgacc tcgaggccga ccacgtccgg cacgtccacc cgcttcggcc
     8641 cggtgctgat gaagagctgc acgcgcgcgc cggtccgctg ctcggtgccg ccagccgggt
     8701 cggtgcggat cgcgctgccc gcggggatcg acgccgaggc ctcgtcctgg cgggtgtact
     8761 cgaagcccgc gtcggcgagc agcttcgccg cctgcttctc ggtcttgccg cgcacctcgg
     8821 gcacgatctt cgtcccgggc ccgctcgaca cccgcagcgt gaccgtcgcg ccctcctcga
     8881 cctcttcccc cgccgccggg tcgtgggaca ccacgctctc ctccggcgcc tcgctctcga
     8941 ccggcttcac gtcgctcttg aggccgagcc gggcgagctc ggtgactgcc tgcggcgccg
     9001 gcttgccgac gaggttgggc acctcgaccc tgtcgtcgcc gcccagtgcg aaggccagcg
     9061 ccgcggcgcc cagcaggagc agcagcacca gcgcgaccca cggccagcgc cggcggcggc
     9121 cgtcctgctc ctcggtgagc tcggtgggag cgatcagcgc accccaggcc gccgtgtcct
     9181 ggcccgggtc gccggagtgg atcgcgtcgc gcgccatccc cagcgcggcg gcgaactcct
     9241 cggcgctcgc gtagcgtgcg gccgggtcct tctggagcgc gcgcatgatc gccgcctcga
     9301 gcgcggggtg cacgtcgggg cgcagctcgc ggatcggcgc cggcgcgtcg ctcacgtggc
     9361 gcagcgcgat cgccaccgcc gtgtcgccgt cgaacggaac ccgcccgacg agcatctcgt
     9421 agagcatcac gccgatcgag tagaggtccg acgcggcgtc caccggctgc ccctgcgcct
     9481 gctccggcga gaggtactgc gccgtgccca tgatcgagcc cgtctcggtc atctccgacg
     9541 cgcccgcgcg ggcgatcccg aagtcggtga ccttgagctg cccgtcgggg ccgcagatca
     9601 cgttgtgcgg cttgaagtcg cggtgcacga tcccgttgcg gtgcgcgaag ccggccgcgt
     9661 cgaggatctg gagcccgtag gcgatcgtgc gctcctggtc gagcggcgcc tcctcgttga
     9721 ccacctggcg cagggtccgc ccgggcaggt tctccatcgc gatgtagtag gtgccgtcgt
     9781 gctcgccgcg gtcgtagatc ccgaccacgt gcgggtgctg tagccccgcc gccgactgcg
     9841 cctcgcgctt gaaacgctcg acgaactggc catcgcgcgc gaagcggcgg tgcagcacct
     9901 tcagcgctat gtcgcggccg agctggcggt cgtgcgcgag gtagacgtcg gccatcccgc
     9961 ccgagccgag ccggcggccg acctcgtagc ggtcgtcgac gagggcgccc tcgacgacct
    10021 cagccaccgt cacccccgag cagcgactgc atgacctgct tcgcgatcgg cgccgcgatc
    10081 gtgccgccct ggccctgcgt gcgctcgacc gtcacggcga tcgccacctc ggggtcgtcc
    10141 agcggcgcga acgcgatgaa ccaggcctgg ttcgaggcgc cgttctgcac ctcggccgtg
    10201 ccggtcttgc ctccggagcg gatgcccgcg agcgcgctcg cggtgccggt cccctcctcc
    10261 accaccttgc tcatcatccc gcgcagctcg tcggccgcct tgcggctgat cacacgcccc
    10321 tgccggctcg ggcgcacgcg ctggcgcacg ctgccgtccg gctccaccac gcggtccacg
    10381 atccggggct tcatcaggat gccgccgttg ccgaccgtcg cggccaccat cgccatctgg
    10441 agcggcgtga cctggagccg ctcctgcccg atcgcgacgc gcccgatgtc gatgtcgtcg
    10501 tcctcgtcga gcagccgctg gccgctgtag atgccgctcg gggtgatctg tgccttcggg
    10561 tagtcgagcg gcggctccga caggaagccg aagcgctcca tgtacttcag catcgtgccc
    10621 ttgccgagct tctcgccgac ctcgccccag accgtgttca ccgactgcgt gagggcgtcc
    10681 gtgaggttga tcgtgccgaa gtcggcgccg ccggagttgg ccagcggcac gccgctgatc
    10741 ggcttgttgt tgcgcccgtc cacgagcgag ccggggttgt agcggccgct gtcgagcgcg
    10801 gccgcggcgg tcacgacctt gaacgtcgag cccggcgggt agcgcgcctg cgtggcgcgg
    10861 ttgaagagcg gcgaggaggc gtcgcggttg agttgcgaga actgctgcgg gatcgagttc
    10921 gggtcgaagt ccgggatgct gaccatcacc ttcaccgcgc cggtgtcggg atcgagtgcg
    10981 acgatcgacc cgcgccgtcc ggcgagctgc tggatcgcca cgcgctgcgc ctgcgcgtcg
    11041 aggtgggtgc gaatgtcgtc gccctccttc acgccgccac cgagctggtc gatcaccgac
    11101 tcgagctcgt cgtgctcgcc gatcagctcg tcgttgtagg agcgctcgat gccggcgctg
    11161 ccgcgcgaga cgaacgagta gccgatcgcg tgcgagaaca gccgcgactg cgggtagacg
    11221 cggtagtagc gcttggtctc gcggttgccg gtgcgccggt tggtggcgag cttggtcccg
    11281 tcgctcgcca ggatcagccc gcgcgggatc ttctgcgcct tcaggatcgg ccgccggttc
    11341 gacggcttct cctcgagctt ctcggccccg aacacggtcc agtacgacgt gaacccgacc
    11401 agcacgccga acatcagcac gaacaccacg aacaggcgcc gcagcggctt gttcatacga
    11461 gccctccgcg ctgcgcggcc gggtcgcggc gtgccttgtc ggagacgatc atgagcagcg
    11521 cgatcaggac gaagttcgcg acgatcgagc tgccgccgta ggagacgaac ggcatcgtca
    11581 cgccggtgag cgggatcgcc ttcacgactc cgccgacgat cacgaacacc tggagcgcga
    11641 acaccgcccc caggccggcg gccagcagct tcgagaagcc gtcagtggcg aggacggccg
    11701 tcttgaacgc gcgcgcgacg atcatcagcg caaccaacaa cacgccgacc gccccgaaca
    11761 gaccgagctc gctcgttatg accgcgtaga tcaggtcggt gtgcgcggcc ggcaggattg
    11821 gcgtgccgtc ggactgctgg atcagcgact ggccgaggcc ctcgccgaac agaccgccgt
    11881 cggcctgcgc gtagaggccc tgcgagatct ggtagccctc cttctcgacc agctccggct
    11941 tgaacgggtc gatccacacg tcgacccggt cctgcacgtg ggagatgcgc tgcgagagga
    12001 accaggcgcc gccgaggaac atgccgagcc cgcccaccac gagcgagatc cgcgacgtcg
    12061 ccacgtagac cagcgcgagg aagccgccga agaacatcag cgagctgccg aggtcgcgga
    12121 tcacgatcag catcagcatc gcgaggcccc agatcacgag cagcgggccg aggtgcttca
    12181 tcgacaggcg cggcaggcgg ccgcggacca gcacgtcgcg cgtctcgctc aggtagctcg
    12241 ccaggaacac gatgatcgcg agcttggcga actccgcggg ctggaactgg accggaccgg
    12301 cgcccaccga caggtacgcg ccgttcgtct gctggccgat cccgggcacg cgcgggagca
    12361 tcaggaagcc gatgccgccg agcgcgatca ggtagcggta gcgctcgagc acgcggtggt
    12421 cgcgcaggaa caggatcgtg acgcagaaca gcgcgaggcc gacgccgaac cagatcgcct
    12481 gctggcccgc gcgcgtgggc gagatccggt agatctcgac gagcccgaac gccgcgagca
    12541 gggcgaccag cgggaacagg tacgggtcgg cgtcaggcag gcgcgcacgg atgaacaggt
    12601 ggccgagcac gcaggccgcg aggaagacgc cgccgtaggt ggcggtcagc tcgttgatct
    12661 ggtagccggc gtcctggcgc gtgatgagga tcgccgcgaa gccggcggcg accagcaggg
    12721 acaccggaac cagggcgaag agctcgcgcg tgcgggcgga catcagcgcg gcgtctccgc
    12781 gcgttcgagc tcgcggatgc gctcctccgc gtcgtcgcgg ccgcgcagct cgtggttcaa
    12841 cagacgcgag cgctggcggc ggtcctcgac cgcgctgacc gggacgctgc tcgagtactc
    12901 gtgcgtgtac aggtcgacgc cgagcggcag ctcgtacggc agcccgcgaa acaggctgac
    12961 ggcgccgcgg tcgtcggctc cgatgaagta gacctgccgg ctgccggcgt agagcccgac
    13021 ggcgacgagg gcgagagcga ggaggaagag ggcggtgccg ccgccgagcc ggggtcgggc
    13081 ggggcggcgc ggggggcgcg aggagtcgtc gccatgcgtg agcgcggccg ggcggtcgcg
    13141 cgccggtccg gcgcgggcac ccgcctcggt gcgggccgct ccgccggcct gcgcgcgcgc
    13201 ccgagcggcc tcctccgcgg agagagtcat cgtgccggcc tcgcgcggat cggcgacgct
    13261 ggcgccgcgg cgcgtctcgt cctcggccac cgcctcgcgc acggccgcgg tggtcacccc
    13321 cgcctgcgtg gcgccgtcgc cgagcgtgtc cgactcgggc tcggcgccct cgtcctcggc
    13381 cacccggaac agcaccgccg tgatgttgtc cttgccgccc gcggcgttgg ccgcgtcgat
    13441 caggccctgg gccgcgtcct cgagcgagcg cgagccgcga aggatctccg ccatctggtc
    13501 gtccgaggcc atcccggaca ggccgtcgga gcagagcagg tagacgtcgc cgtcgcgggc
    13561 ggtatgcgtg aaggtgtcga ccttcacgtc cgactccgga ccgagcgcgc gcgtgatgat
    13621 cgagcgctgc ggatgcacct cggcctcctg cggggtgagc ttgccctggc gcaccagctc
    13681 ctccaccagc gagtggtcgt cggtcagacg ctcgaacgca ccgtcgcgca gccggtacag
    13741 gcggctgtcg ccgacgtggc ccaccgccac ctcgcgcccc gagaccagga ccgcggtgaa
    13801 cgtggtgccc atcccggcgt gctcgctgtc gctctgcgcc atgtcgtaga tgcgccggtt
    13861 cgcggagcgc gcgaccgtgc gcaggctctc ctccgcgcgc gcacccgggt cctccatctc
    13921 cgccagctcc tcgaccgcca tccgcgacgc gacctcgccc gcgcgcgcgc cgcccatccc
    13981 gtccgcgacc gcgaacaggg gcgcacgctc gaagaacgcg tcctcgttgc tctggcgctg
    14041 gcgccccacg tcggtgaggc tcgccttctc gactatccgg aggctcattc ctggtagcgg
    14101 tactcggtgt cgcctatgcg aacggtgtcg gccacgcgca gctgctcggg cttgcgcagc
    14161 tgccggccgt tgagaaaggt gccgttggtg gatcccatgt cctcgacgta catgaactgg
    14221 ccgcgcggga agatccgtgc gtgcgcggac gaggcgtaag gatcgtctat ctgcacgtcg
    14281 gcggtcggcg agcggccgag cgtggcgctg tcgcctatct cgaaggccgt cccgggctcg
    14341 tagccgagcg ccgccaccac ctcgaggcgg ggccggacgc cgctgcgcag gtccatcgcc
    14401 gagccgcggt cgcggcggcc gctggcggcc ccctcgtcgt cctcgtcggg catccgcccg
    14461 gcaccggcca tgtccttcag cgcgctgcgc acgacccaga ggaggaacag gtagagcacg
    14521 gccaggaagc cgaacttgag cagcacggcg atcgggtctg tcacgcggcg gcagctccca
    14581 tggctattcg agctcgaacc tgaaccgggc gaggccgaac acgacctcgt cgccgggctc
    14641 gagcgccgcg tgctccacgc gccggccgtt caccttcacg ccgttcgtgg agccgaggtc
    14701 cgcgaccacc cagcggccgt cctcgttgcg cagctccgcg tgccggcgcg agacgttcgc
    14761 gtcctcgacc gtgatgtcgc agtcgcgcga gcgcccgatg accaggcggc tgcccgagag
    14821 cacggcccgc ttgtcctcgc cgatgaacag cgcgcggccg ccgcgcggga cgtcgcgcgg
    14881 cgcgagcttg cgcggcgagc tcgcgggcga gtagaccatc gtgtggccaa agtcgccggc
    14941 ggcggcgccc gcgtcggcgg gccagtccgg cgcctcctcc tccggggcct ggatgagctg
    15001 cgcctggatg ccgaactcgc cgaggctcag gcggtcgtcg gtctcgaact cgatcgtggg
    15061 ccgcgtgacc agcgcgagcc cctcggtgcg cgcgtgctcg agcaggtagt cggagagctc
    15121 cttcttcagc ccgtcctcgt agccctcgaa gtgaccacgg tcctcggggg agagccagac
    15181 ggtgtagagg ttcggcacgt aggtgtgcga gatcgaccgc gtctggttgt cctgcatctc
    15241 cttggcgagc ttgcgcgcga tctcgaccgg ctcgaccttc gagcggaacg cgcggctgaa
    15301 ggctccgccg acgagcccct ccagcttcgc ctcgaggttc ctgaggacac tcacgggcgc
    15361 atgatcctag ggaacgggtg ggctgtcgcc ggggcgggga cggaggcccg accagaccgt
    15421 cgcgagcgcc acggcggcgc cgatggccgc ccccgcggcg gcgccccggg cctcgtccag
    15481 aggcacgtcg ccggcggcca gctcggctat cccgcggtgg gccgcgacca atgcggcggc
    15541 ccagaccagc gcaccgatcg cgtcaagcgg gagggagcgg ccgcgcacga caagcggcag
    15601 cgcggcggcg aaggcccccc acgcgagcgc cacgaggatg aggggcgaga gcaccagcgg
    15661 cccgatcgcg tccgtcatcg cgccggacag cgaccccgcc cactccccgc gcgagctggc
    15721 gtcctgcggc ggcccgaaca gcagctcgtc ggagctgagc agctcggcgg cggcgagcca
    15781 caggaagccc gccgcgccca ggccggcgcg ccgccaggcc gtcgaggcga gccccgcggc
    15841 gccgacgaac gcggggccga tcccgatcac gccgagcagc ggagcggcca ccggcgccga
    15901 ccacaggcgc ccgccgcgca ggagcagcac cggcacggca gccagcgtga gcccgaccac
    15961 cagcgcggtg ccgtcgagca gctcggagcc gagccagccc gtgaccccga tggcggccgc
    16021 cagccagccg agccgcggca gcagcgcgac ggcgaccgcg gtggccgccg cccacgcggc
    16081 gaccgggaag gtcgggtcgg ggccgagcgt ctcgattccg gccagcacga gcgccgcggc
    16141 ggcggttccc gcgagcgcgc gcgtgacgag gtccagggtg cgctcggacg ggagcgcgac
    16201 cgccgccggc agcacctcgg tcctcgggat gccgacccgg tccagcgtct cgggcgcggc
    16261 caggccgccg ctgtcgccga ggtggtcggc ctcgcgcacc agcgcgtcgc gcagggcgcc
    16321 cagctcgggg cggcgctcgg cgaccgggtc gagcgcggcg tcgatccagg cgcacatgtc
    16381 gggcgggagg tcggggcggt aggccgagag cagcgggagg cggcggccca ggcggcgcgc
    16441 ggtggcggcc ggcgtcgggc cgcgcacggg gttcacgccg gcccaggcct cgtacagggt
    16501 gagcgccagc gagtacacgt cggcggcggc gctcacgcgc gcgccctcgg cctgctcggg
    16561 ggacatgtac gcgagcgtgc ccaccacgtc gcccgtgcgc gtgagcgggt cgcccaccgc
    16621 gagctgggcc acgccgaagt cggcgagctt cgcgaacccg gcccccgccg ccggctgcgc
    16681 caccacgatc acgttctggg gcttcacgtc gcggtggacg accttgcggt cgtgcgcgtg
    16741 ggcgagcgcg tcgcagagcg ccacgccgag ccgcgccacg tcgcggtccg agaccgcccc
    16801 cgcgcgcacc agctccgcca gcgtgcgtcc gggcacgagc tccgagacca ggtagaccgc
    16861 ctcgtcgtcc tcgcccagct cgtagagcga gacgatcccg gggtggttga gccgagcggc
    16921 cacgcgcgcc tcgcgagcgg cccggtgggt gaccgggtcg tccccatcgg cctggatgac
    16981 cttcacggcc acctcgcgct cgagcttctc gtcgaacgcc agccagacca ccccgaaccc
    17041 gcccgcgccg agcttgcgct cgagccggta gcggcccagc acgagccggt ccgtgggcgc
    17101 acggcgtccg ccgccggaca gcggctcggt cgcctcgatc gcaggctcga agcggcgcgg
    17161 cgggctcgcg ggggcctccc cgaccacgcg cgtgcgctcg tcgggcgggc caagttgctc
    17221 tgtggtcacg ggacgttcca ctgcggcttt cgtgttcggg atggcagggc ggctacctcc
    17281 cgctgggagc ccgcgcccgg cttgcggccg aggctcccgt aatactgaac caatagcctg
    17341 ccacctaggc gcgggctgga gcactccttt gtccttcttc gacgaccgag aagacaggcc
    17401 cggcaggacg gcaccgcggc ggcctccggc ccgcgggccc ggcaccgatc gccagaccct
    17461 gatggtgcgg cgcgccatcg cggccgccgg cggcctcgtc ctgatcatcc tgttcttcct
    17521 cttcgtgaag agctgccgcg agaacgcccg cgaggacgcc ttccgcaact acggccgcga
    17581 cgtggccgcg ctggtgcagg agtccgacca ggcgagccag cagctcttcg gcctcctcac
    17641 gcggcccggt cggcgcagcc cgctcgagct gcagcaggcc gtcaacggct accacagcga
    17701 cgccgcgcag ctcgtcgacc gcgcccgcga caccgaccac cccgacgagc tcgacacggc
    17761 gcagcgttgg ttcgtcgagt cgctccagtt ccgccgcgac ggcatcgagc ggatcgcgcg
    17821 cgagctgccc accgcgctcg gcgaccaggg gcgcgaggag gcgaccgcgc gcatcacggt
    17881 caccatgcgc agcttcctca cgagcgacgt cctctacagc gaccgcttca tcccgcacct
    17941 cacgaacgag gtgaagaagc agaagctgac cggtgaggtc aacatcccgc gcagtcgctt
    18001 cctccccgac ctccagtggt tgcggccgac cgtggtggcc gagcggatcg acgcgatccg
    18061 cggcgggggc ggcgggacgg gcacggcggc gccgggcctg cacggcacgg cgctcgtgag
    18121 cgtgaccgcc aagccgggcg gcaaggcgct cgtcccgggg ggcgcgacgg acatcgccgt
    18181 gtcgccgcgg ctctcgttcg acgtggcggt ctcgaacggc ggcgagcacg aggagcgcga
    18241 cgtgaccgtc agcctctcga tcgccggcgc gggcaagccg ctcgtgcgcg atcagcgcct
    18301 gccgtcgatc aagccgggcg agcagaagac cgtcagcatc ccgctcgcgg ccacgccgac
    18361 caccggccgc cccgtcacca tcaccgtcga ggtgaagccg gtgccgggcg agaagaaggt
    18421 cgacaacaac aagcagcgct acccggccat cttcacgcgc tagggcgacg gtagcctcgg
    18481 caccgatgga cgatctcagc acgacagagg gcatcgccgc gctggccgcc ggcggcgtgg
    18541 cgctcctcgc gctgatcctc gcgacggtgc tcgccttcaa gctgcggcgg atgcgccagg
    18601 cccagacggc ggtgctcggc gagtacggat cgcgcgacct cgtgtcgcac gcggagcggc
    18661 tcgagagcgg gttcacgcag ctccgcgact gggtcgagga gtcgatgcac tcgatcgact
    18721 cgcgcatggg gcaggtggag gggcgcgtcg acggctgcat cgcctaccac tcgctgatcc
    18781 gctacgacgc ctacaacgag ctgtccggcc accagtcgag ctcgatggcg ctgctcgaca
    18841 gccaccgcaa cgggctcgtc ctatcggcga tcgtgcaccg cgaccaggcg cggctgtacg
    18901 tgaagcaggt gcagggcggc gagggcgagg tggcgctctc gccggaggag caggaggccg
    18961 tcgagacggc gctcgcctcg cccctgcgcg ccgcctgagc cgcatgcggg tcgcattcct
    19021 cggtccggcc gggacgtaca ccgaggaggc gctgcgccac tccgccccgg cggggactga
    19081 gttcgaggag gtgccgtacg caacggtcta cgagaccgtc atggcagtcc agtccggtgc
    19141 cgccgagcgc gcggtggtgc cgatcgagaa ctcgctcgag ggatccgtca ccgcgacgct
    19201 cgacgcgctc gccggcgagg ccgatcgggt gcggatcctc gcggaggtcg tgcggccggt
    19261 ctcccactgc ctggtcgcac gcggcggcgt ggaggtcgag gcggtcgagc gcgtgctctc
    19321 gcacccccag gcgctcgcgc agtgctcgga gctgctgcgc gggcggctcg cgcacgcgga
    19381 gccggcgtcg gcgccgtcga cggctgacgc ggtgcgcacg gtcgccgagg ggtccgagcc
    19441 gtgggcggcg atcggcacgc ggctggcggc cgagatgtac ggctgcgagg tgctggccga
    19501 gggggtcgag gaccgctccg acaacctgac ccgcttcgtc tggctcgcgc cggaggggac
    19561 cgagccgccg cacgagccgg cgaagacgtc gatcgtgttc tgggggttca acgacgagtc
    19621 gccgggcgct ctcgtcggag tcctcgccga gctggccgat cgcggcatca acctgaccaa
    19681 gatcgagtcg cgcccacggc gcgtgcagct cggtcactac atgttcttcg ccgacctcga
    19741 cggcggggtg tccgagccgc ccgtgcgcga cgcgctcgcg gcgctcgggg agcgggttca
    19801 ggcgctcaag gtgctgggtt cctaccccat cggtggttag actggatcgc gtcgtggcga
    19861 tcgcgtcgag taccggctct gggtccaaca gccgcgtgct cgtcctgaac gcgtcgtacg
    19921 agccgatcaa cgtgtgcacc gtccgccgcg ccgtcgtgct cgtgctcaag cagcgcgccg
    19981 aggtgctcga gcgctcggat cgcgacctcc acgcggagag cctgacggtg gcccggcccg
    20041 cggtgatccg cctgatgacc tacgtgcgcg tgccgcgcga cgcacattcg cgcaagatca
    20101 cgcggcgggc gatcttcgcg cgcgacggct gggcgtgcca gtactgcggc gccggccacg
    20161 gcacgctgac ggtggaccac gtgatcccgc gatcgaaggg cggccactcg ggctgggaca
    20221 acatcgtcac ctgttgcgcg ccgtgcaaca ggcgcaaggg cgaccagctc cccaaggtgg
    20281 tcgggatgca cccgcgccgg gcgccgacgc cgccgagccc cacgatcttc gtgcacgtcg
    20341 cggtcacgcg cgtgccggac acatggcagc agtacctgcc cgccgcggcc tagccgcgct
    20401 gctgctcgag cttgtacgcg taggcgagcg acacgagcgc gcagtggcgg tgccacccag
    20461 atagcgagcg cccctcgtag tcgtcgatgc cgagctcgcg cagctgctcc cagccgaggt
    20521 cgacgttccg cgggatgagc gcgcaccggc cgagctccca catcggcgta gacgggctct
    20581 ggcgcagcgg tccgcccacg agccagtagc cggagggacg gtcgacgccc agcggccact
    20641 cggccagcag cgaccggttc cgtgcacgct cgggctgata ccagccgtcc gcgtgcggaa
    20701 tgcgccagcg cagcgccagc cgcgtctcgt ggttcgcgcc gaacggctgc atgacgaccg
    20761 tgccctcctc gaattccgac agctccaccg agtggcgggg cacgggcagc acgaacgaga
    20821 gcccgcggac gtcctcgatg cgcctgaggt cgtcctcgca ctcgtcgacc tcgtcgacgt
    20881 acagccggtc gctgaccacg ggcgcgtgaa gggcgctctt ccagggggat gcttccgaga
    20941 gccccgcgcg gtcgagcgcg tcgcggaccc gctcgagcag gcggctgcgc cagcacccgt
    21001 agcggatgct gtcgaagtgc gtgcggtgca cctcggccgg gacctgcgcc aagtgctcgg
    21061 gcaccgagag ccaggtgtgc gggaggaaca tcgaccatcc caccgggagg gagacggccc
    21121 ggccgcggcg gcgcccgacg gcgtgcaggg agatgacgcg ctggcagttc atctgcctac
    21181 cgctcgccgg cgagtactgc agcgcgacgc ccggcgactt gtcgcctcgc ttcgggacga
    21241 cggtctcggt gaacatccac gcctggatgt cgtcgaagct ctcggcggcg tagcgcgcga
    21301 ggccgcgcat cacgacgacc ggatcccagg tcgcgtcgac gaggaagccc tggaggcgct
    21361 cgtagagagc gcggtcgcca tcgatcgcct cctggatccc gcgcagcgtc gagcgccgct
    21421 tcggccctgt cctcagaagt ccggtcacgt agcggagcgc cgcgtcgcgg tggctgtcgc
    21481 gcgtccagct tccgacgatc cgatcttgga agtcggtcag ttccggcgag atcaccaccc
    21541 tgcggatcgt agtcccaggt ccaactttgc acgacattgg cgactttgaa ggcccttact
    21601 tcctctccga gaatcgtaag tgcggtagtg tggcgcgctg cggcacttct tttgggagac
    21661 ttaggtcgat gcgcagggtt caggtggtcg gattgctcgt atgcgtgctc gcgctggcgt
    21721 gggcgccggc ggcgcgcgcc gagcaggcca tcgacgacga ccccaccgtg agcggtccgt
    21781 ccaacaccgt cgagaccgtc atcggggccg gctgggaggg cgacctgctc cgcacgctcg
    21841 ccgagggaca gggtcgcgag gaggtcaacg gcgccgagtg gaacgcgccc ctgagcggcg
    21901 gaacctacga ggtgtccgtg tggatcccgc gcggctacgg cgggacctac gtccgctacc
    21961 tgatcgccca caagggcggc gtatccgagg tccgcctgcg gcaggctgcg cacgaggacg
    22021 tctgggtcac gctggggacg ttcgagatcg acggcccgac ggccacggtc cgctcgaccg
    22081 acgcgggcgg gatcgccggc cagagcatgg tctgggacgc ggttcgatgg accaaggtcg
    22141 accccgcggt cagcacgccg gatccggccg ccgacgagca cctcgtcgac gaccccgtga
    22201 tcatcggccc agccgagtac gtgaccacct tcgtcggggt ggggtacgac ggcgacctgc
    22261 tgaggacata cgcagagggt tccgatcacc gccagatcaa cggagccgag tggcgcacgc
    22321 ctctgacgtc gggtcgcttc cgggtccagg tctacatccc gagcgagcac ggcgagggac
    22381 ccgtgcggta cttcgtcccg cacgtgggcg gtgagcgcga gatcctgatc gaccagagcg
    22441 gctactcgaa cgtgtgggtc gacctgggcg agttcgacct cgccggcccc gagggcgtgg
    22501 tccggtcgac ggacgcgacg ggcacgcgcg ggaacgaggt ggcctgggac gccgtccgct
    22561 gggtccgggt gcccagcgcg ccgcaggcgc ccgcgaccga tccgggcggc gctccctcgt
    22621 cagaccaacc cggttccgat cagccgcaga cgggcccccc ggcgacggcc aactccgacg
    22681 gcgcggccga cccggacggc gacggcgtcc cgaacgttct cgacgcgtgc gacggcgaac
    22741 accgcggcgc gctcgactcg aacggagacg gctgcccggg gccgaccaag gtcctggcgc
    22801 gtcagacgcg ccgga
//
LOCUS       DASVPL010000318        11986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_38222, whole genome shotgun sequence.
ACCESSION   DASVPL010000318 DASVPL010000000
VERSION     DASVPL010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38222"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..7373
                     /locus_tag="VF517_18260"
     CDS             54..7373
                     /locus_tag="VF517_18260"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8054933.1"
                     /translation="MEAEGAEELVAALGELGCEAQVAACDVADRAALEAVIAGIPDEH
                     PLTAVVHAAGVLDDGVIDSLDGERLRAVMTPKVDGAIALHELTAGLGLTQFVLFSSVA
                     STFGNQGQGNYAAANAFLDALAQRRRAEGLPATAIAWGAWEATGMAARSSDADRSRLR
                     RFGIAELPDEQGLALLDAARFAPQPLFAAVRLDASTLRSQAKSGTLPAILRGLVRVPA
                     RRASTGSLARRLAAAPEAEWQAIVLEVVRGHVASVLGHSSAQSIDPERPFKELGFDSL
                     SAVELRNRLGQATDLKLTATLVFDHPTPAAVAEYLRSQVEDAERAAPAVRRSAAHTDE
                     PIAIVGMSARYPGGVRSPEDLWELVAEGRDAIGEFPDDRGWELERLFDPDPDHPGTSY
                     AREGGFLYDAGEFDAGFFSISPREALALDPQHRLALESAWEAFEHAGIDPARLKGSQT
                     GVFTGIMYSDYGSTGMPPELEGFLGVGASLLSGRLAYTFGLEGPAVSVDTACSSSLVA
                     LHMAAQALRSGECELALAGGATVLATPSTFIQFSRQRGLSPDGRCKAFGADADGVGWS
                     EGAGLLVLERLSDARANGHEVLAVVRATAVNQDGASNGLTAPNGPSQERVIREALASA
                     GLSPSDVDVVEGHGTGTTLGDPIEAQALLATYGRDRTDGPLRLGSIKSNIGHTQAAAG
                     VAGVIKMVEAMRHGELPPSLHCEEPSPHVDWSSGEVRLLTEAEPWERGDRPRRAGVSS
                     FGVSGTNAHVILEEAEAAEEAPAERSEPPALPFLVSARGEIALRTQAERLRAWLDERP
                     ALEPLDVAFSLAETRARLERRAVVVGSSREELAAGLHALASGQPAEGVVEGVARGGRS
                     VFVFPGQGSQWEGMALELMDAAPVFAESLRACGEALSKYVDWSLEDVLRGADGAPSFE
                     RVDVVQPALFAVMVSLAALWRSFGVEPSAVVGHSQGEIAAAHVAGALSLDDAARVVAL
                     RSQAVRDDLAGHGGMASVALSPEACEERIERFGERVSLAAVNGPASVVVSGEDAALDE
                     LLAECESDGTWARRIPVDYPSHSAAVEALEERLDRDLGPIEPQPGTVPFFSTVTAEVV
                     EGDTLDAAYWYRNLRNRVRFNDAVAALIEDGAGAFLEMAPHPALTVGIAAAAEAADAA
                     ERVAAVASLRRDEGGLGRFLTSLAEAHVHGVPVDWAPLYAGTGARRVDLPTYAFQRER
                     FWIESVRGVGDLAAAGLGTVDHPLLSAELALPGDRGTSFTGQVSLSQQPWLRDHAVFE
                     RVLFPATAFAELLLAAGRALGCEVVEELTLEAPLGLGESDELALHVLVGEDDGQGRRE
                     VEVHSRRSVDPEANGNGNGSKWSRHAVGVVSHDASPAGDAVSGLATEAWPPADAEPID
                     VDSMYDRLADAGFGYGPAFQGVVAAWRRGEETFCELALAEEVAQEGAGFGVHPALLDA
                     SFHAVVDRLGSESDGRSLPLPFALSGVRVHRAGASALRVGLRVAGDGSAVSLAAVDGD
                     GQPVLEIESVAVRPIDAERLGAAARVGADSLFRGAWVELALDGDARPPERYAVLGGGV
                     ELPGDVPDHPDLDALIAAIEGGAEVPDVVFVSAADVVAIDAASNVDEAAAAGESVVGL
                     LSFLQAWLAADALSESRLTLVTRGAMGVRDGEAPDPARSAWWGLARSAQSEHPGRLVL
                     VDLDEHADTREVDWSALLGADEPQLALRRGGVYGFRLVRLQTSLALVPPAGEDAWQLA
                     TPRHGTLEDLALVESERAKRALEPDEVRIAVRAAGVNFRDVLIAIGQVDDPEPIGGEG
                     AGVVLEVGDAVTDLAVGDRVVGVMPQSLGPVAVSDRRVLAKLPEDWSFVEGASVPIAF
                     MTAYYGLLELGELGEGQRVLVHAGAGGVGMAAIQMAQDRGAEVYATASPAKWDVLRGL
                     GLDDDHIASSRDLDFRDRFLAATDGEGVDVVLNALAREFVDATLELLPRGGRFVEMGK
                     TDIRDADQVRRDHPGVEYRVFDIIRDISSEYAGKVLGGVMELFERGALRHLPIRAWDV
                     RHAASAFRHLAEGRNVGKVVLTIAQSLDPEGTVLITGGTGDLGGRVARHFAGEHGVRR
                     LLLTSRRGPDAPGATELVEELRELGADASVVACDAADRDDLSALLASIDAEHPLTAVI
                     HTAGVLDDAVIESLTGEQIERVMRPKVDAALLLDELTRGSELAEFVLFSSQSGTMGAP
                     GQGNYAAANVFLDALAERRRAQGLPAKSLAWGLWSDARGMAGQLDEADAARLARLGVA
                     VMRDELQLLDAAREAAESVVVPTRFDMSGLRSAAEAGTLPPLLQDLVRRPQRARETGR
                     SLDQELAGVPEDEREDRVLAVVSEQVAAVLGHSSAEAIDPERTFKGLGFDSLGAVELR
                     NRLMRVSGQRLPSTLIFDHPSPVAVAQYVLEKLALPPAPDAEPSREAGDEIEIREESD
                     GLDELDADELIRMAKES"
     gene            7373..>11986
                     /locus_tag="VF517_18265"
     CDS             7373..>11986
                     /locus_tag="VF517_18265"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8054934.1"
                     /translation="MSPSHEELTDALRSSIRDREQLREENRRLAAKEAEPIAVVGMAC
                     RFPGGVRSPDDLWRVLVSGTDTISEFPTDRGWDLDRIYDPDPDARGKVYSRYAGFADD
                     VTDFDADFFEISPNEALAMDPQQRVLLEVMWEAIENAGIDPASLTRTPSGVFVGASAP
                     DYAGRLAAEVEGYRMTGSLPSVISGRVAYELGLEGPAVTVDTACSSSLVATHLACQAL
                     RRGETSLALAGGVTILATPWMLVEFSRQRGLARDGRCKSFADSADGTGMSEGAGVLVL
                     ERLSDAHRNGHRVLAVIRGSAVNQDGASNGLSAPNGPSQERVIRDALAAADLSAAEVD
                     AVEAHGTGTTLGDPIEAQALIATYGQERSDGPLRLGSIKSNIGHPLAAAGVAGVMKLV
                     LALRHGLLPPTLHVDEPSSHIDWTEGEIDLLTEAQPWPRGERPRRAGVSSFGISGTNA
                     HAIIEEAPELEEPEAASSEATEPPVMAWLVSAKSEEALRAQARELRAHVEERPELSPV
                     DIAFTLATGRARLLKSAVAIGADRDALLAGLDAIERGERPAGELRRGKTAFLFTGQGA
                     QRAGMGRELAEAFPAFAEALDEVCAELDRHGDRPLKELLFAEPGSDAAALLDRTEYTQ
                     TALFAVEVALFRLVESLGMKPDYLAGHSIGELTAAHVSGVLSLPDACALVAARGRLMG
                     ALPDGGGMLAIRAAEDEVAESLEGFEGRLSIAAVNGPRAIVVSGEAEALDELEPVWKE
                     RGADTKRLRVSHAFHSPLMEPMLDEFRKVAEGRRFSRPRIPIVSALTGERIASEKISD
                     PDFWVRHVREPVRFADAVTALHGAGVTRFLELGPDGVLTAMAGESLDPDDRERAVLVP
                     ALRSKRGDGETFVAFLAAAHAAGAAVDWRAFFDGSGARPVDLPTYAFQRKRYWLVPAA
                     GAGDLAAAGQLAAGHPLLGATVRLAGERDGWLLTGRLSLETHPWLADHAVMDTVLLPG
                     TAFVELALAAARRVGAGGIEELTLVAPLVLDGERALQVAVAEPGDDGRRAVEVYSRAQ
                     GGADDGADDEEWTLHASGLLGAAGAEGAHAALEAFAAASWPPDGAEELDVEDLYDRLA
                     EAGYDYGPAFQGLQSAYRAGEELYAEVALDGEEGEGGGFGAHPALLDAALHAMVLGRE
                     ADASPEVPFSFSGVRLEHEGAASLRVRIETSGDGETTRIGLQALDETGAPVLAIDALE
                     MRPVDRAALRAGASVGRDGLFALGWVEVEAADPGGAAPGLALIGAADELGSQLDATGY
                     SDLATLERAIADGAEAPAVVLARAGAADGPLAGSVHELAERTLELLQDWLDSEALADA
                     RLVLLTDRALAVTEGEAPNLAHAALAGLLRSAQSENPGRFALVDLDGSDASATGLHAA
                     LASEEPELALRDGTLHAPRLSRSGALAVPAGGSAWRLAIERKGSLEDLALVASEAAEA
                     PLGEGQVRVAMRAAGLNFRDVLIALGMYPGEAPLGSEGAGVVVEVGPGVERFAPGDRV
                     MGFVEEAFGSHAVGDERTLVSIPEAWSYTEAAAVPIAFLTAYYG"
BASE COUNT         1455 a         4314 c         4614 g         1603 t
ORIGIN      
        1 acctcgcctc cgagcacggt gcgcgccacc tcgtcctgac cagccgccgg ggcatcgagg
       61 ccgagggcgc cgaggagctc gtcgcggcgc tcggcgagct cggctgcgag gcccaggtgg
      121 ccgcatgcga cgtcgccgac cgcgcggcgc tcgaggcggt gatcgcaggg atccccgacg
      181 agcatccgct gaccgcggtc gttcacgccg ccggcgtgct cgacgacggg gtgatcgact
      241 cgctcgacgg cgaacgcctg cgagcggtga tgaccccgaa ggtcgacggg gcgatcgccc
      301 tgcacgagct gaccgccggc ctcgggctca ctcagttcgt gctgttctcc tcggtcgcct
      361 ccaccttcgg gaaccagggg cagggcaact acgcggcggc caacgccttc ctcgacgcgc
      421 tcgcgcagcg ccggcgcgcg gaggggctgc ccgccacggc gatcgcctgg ggtgcgtggg
      481 aggccaccgg gatggccgcc cgctcgagcg acgcggaccg ctcgcgcctg aggcgcttcg
      541 gcatcgcgga gctacccgac gagcagggcc tcgcgctgct ggacgccgct cgcttcgcgc
      601 cgcagccgct gttcgcggcg gtccgcctcg acgcatccac cctgcgctcg caggcgaagt
      661 ccggcacgct gccggcgatc ctccgcggac tggtgcgcgt gcccgcgcgc cgggcctcga
      721 cgggttcgct ggcgcgacgg ctggccgccg cccccgaggc cgagtggcag gcgatcgtgc
      781 tcgaggtcgt ccgcggccac gtggcgagcg tcctgggcca cagctcggcc cagtcgatcg
      841 atccggagcg ccccttcaag gagctcgggt tcgactcgct gagcgccgtc gagctgcgca
      901 accgcctggg ccaggcaacc gacctgaagc tgaccgccac gctcgtgttc gaccatccca
      961 cccccgcggc ggtggccgag tacctgcgct cgcaggtcga ggacgcggag cgcgccgcgc
     1021 cggcggtccg gcgctcggcc gcgcacaccg acgagccgat cgcgatcgtg ggcatgagcg
     1081 cccgctaccc gggtggggtg cgatcgcccg aagacctctg ggagctcgtc gccgaggggc
     1141 gcgatgcgat cggcgagttc cccgacgatc gcggctggga gctcgagcgc ctgttcgacc
     1201 cggaccccga tcatccgggc acgagctacg cccgcgaggg cggcttcctg tacgacgccg
     1261 gcgagttcga cgccgggttc ttctccatct cgccgcgcga ggcgctggcg cttgacccgc
     1321 agcacaggct ggcgctggag agcgcgtggg aggcgttcga gcacgccgga atcgacccgg
     1381 cgcgcctcaa gggcagtcag acgggcgtgt tcaccggcat catgtactcc gactacggca
     1441 gcacggggat gccgccggag ctcgaggggt tcctcggtgt cggcgcgagc cttctctccg
     1501 gtcgcctggc ctacaccttc ggtctcgagg gcccggccgt ctcggtcgac accgcgtgct
     1561 cgtcgtcgct cgtggcgctc cacatggccg cccaggcgct gcggagcgga gagtgcgagc
     1621 tcgcgctggc cggcggcgcc acggtgctcg ccacgccgag caccttcatc cagttctcgc
     1681 gccagcgcgg gctatcgccg gacgggcgct gcaaggcctt cggggcggac gcggacggcg
     1741 tcggctggtc cgagggcgcg ggtctcctgg tgctcgagcg gctgtcggat gcgcgggcga
     1801 acgggcacga ggtgctggcc gtggtgcgcg cgaccgccgt caaccaggac ggcgcgtcga
     1861 acgggctgac ggcgccgaac gggccgtcgc aggagcgcgt gatccgcgag gcgctcgcga
     1921 gtgccggcct gtcgccatcg gacgtggatg tggtcgaggg gcacggcacg ggcaccacgc
     1981 tcggcgaccc gatcgaggcg caggcgctgc tcgcgacgta cgggcgcgac cggaccgacg
     2041 gcccgctgcg gctcgggtcg atcaagtcga acatcggcca cacccaggcg gcggccggcg
     2101 tcgccggcgt gatcaagatg gtcgaggcca tgcgccacgg cgagttgccg ccgtcgctcc
     2161 actgcgagga gccctcgccg cacgtggact ggtcgtcggg cgaggtgcgg ctgctgacgg
     2221 aggccgagcc ctgggagcgg ggcgatcgcc cgcgccgcgc gggggtctcg tcgttcgggg
     2281 tctcgggcac gaacgcccac gtcatcctgg aggaggccga ggccgcggag gaggcaccgg
     2341 cggagcgctc cgagcccccg gcgctcccct tcctcgtctc ggcccgcggc gagatcgcgc
     2401 tgcgcaccca ggccgaacgc ctgcgggcct ggctcgacga gcggccggcg ctcgagccgc
     2461 tcgacgtcgc cttctccctg gccgagaccc gggctcggct cgagcgccgg gcggtggtgg
     2521 tgggctcgag tcgcgaggag ctcgcagccg ggctccatgc gctggcgagc ggccagccgg
     2581 ccgagggcgt cgtcgagggc gtggcccggg gcggccggag cgtcttcgtc ttccccggcc
     2641 agggctccca gtgggagggc atggcgctcg agctgatgga cgcggcgccc gtgttcgccg
     2701 agtcgctgcg ggcctgcggc gaggccctct ccaagtacgt cgactggtcg ctcgaggacg
     2761 tcctgcgcgg agccgacggg gcgccctcgt tcgagcgcgt cgacgtggtc cagccggcgc
     2821 tcttcgcggt gatggtctcg ctggcggcgc tgtggcgcag cttcggtgtc gagccctccg
     2881 cggtcgtggg ccactcccag ggcgagatcg ccgccgccca cgtggccggg gccctgtcgc
     2941 tcgacgacgc cgctcgcgtc gtggctctca gaagccaggc cgtccgcgac gatctcgccg
     3001 ggcatggcgg gatggcctcc gtggcgctct cgcccgaggc ctgcgaggag cggatcgagc
     3061 gcttcggcga gcgcgtctcg ctcgccgccg tgaacggccc ggcctcggtc gtcgtctccg
     3121 gcgaggacgc ggccctcgac gagttgctcg ccgaatgcga gagcgacggg acctgggcgc
     3181 ggcggatccc ggtcgactac ccctcgcact ccgcggcggt cgaggcgctc gaggagcggc
     3241 tcgaccgcga cctcgggccg atcgagccgc agccgggcac ggtcccgttc ttctcgacgg
     3301 tcaccgccga ggtggtcgag ggcgacaccc tcgacgccgc ctactggtac cgcaacctgc
     3361 gcaacagggt gcgcttcaac gacgccgtcg cggcgctgat cgaggacggt gccggagcgt
     3421 tcctcgagat ggctccgcac cccgcgctca ccgtcgggat cgccgcggcg gccgaggccg
     3481 ccgacgccgc cgagcgggtg gccgccgtgg cctcgctgcg ccgcgacgag ggcggcctcg
     3541 ggcgcttcct cacgtcgctg gccgaggccc acgtccacgg cgtcccggtc gactgggccc
     3601 ccctctacgc gggcaccggc gcgcgccggg tcgacctccc cacctatgcc ttccagcgcg
     3661 agcggttctg gatcgaatcc gtccggggtg tcggcgacct ggcggcggct gggctgggga
     3721 ccgtcgatca cccgctgttg agcgcggagc tggcgcttcc gggcgaccgg gggacgtcgt
     3781 tcaccggtca ggtctcgctg tcgcagcaac cgtggttgag ggatcacgcg gtgttcgagc
     3841 gcgtgctgtt cccggcgacg gccttcgccg agctgctgct ggccgccggc cgcgcgcttg
     3901 gctgcgaggt ggtcgaggag ctgacgctgg aggcgccgct cggcctgggc gagagcgacg
     3961 agctggccct ccacgtgctg gtcggggagg acgacggcca ggggcggcgt gaggtcgagg
     4021 tgcactcgcg tagatccgtc gaccccgagg ccaacggcaa cggcaacggg agcaagtggt
     4081 cgcgtcacgc cgtcggcgtc gtcagccacg acgcgagccc ggccggtgac gcggtgagcg
     4141 gcctggccac ggaggcgtgg ccgccggcgg acgccgagcc gatcgacgtg gactcgatgt
     4201 acgaccggct ggccgacgcc gggttcggtt acgggccggc gttccagggg gtcgtcgcgg
     4261 cctggcggcg cggtgaggag acgttctgcg agctggcgct cgccgaggag gtcgcgcagg
     4321 aaggcgccgg cttcggcgtg catccggcgc tgctcgacgc ctccttccac gcggtggtcg
     4381 accgcctggg gtcggagtcg gacggccgca gtctgccgtt gcccttcgcg ctgagcgggg
     4441 tgcgggtgca tcgcgccgga gcgtcggcgc tgcgggtggg gctcagggtg gcgggcgacg
     4501 ggagcgcggt gagcctcgcg gccgtcgacg gcgacggcca gccggtgctg gagatcgagt
     4561 ccgtggccgt gcgcccgatc gacgcggagc gtctgggcgc ggctgcgcgc gtgggcgccg
     4621 actccctgtt caggggcgcg tgggtcgagc tcgccctcga cggggacgcg cggccgccgg
     4681 agcggtatgc ggtgctcggc ggcggcgtcg agctgcccgg tgacgtgccg gaccaccccg
     4741 acctcgacgc gctgatcgcg gcgatcgagg ggggcgccga ggtccccgac gtcgtgttcg
     4801 tctccgcggc cgacgtcgtg gcgattgacg cggcctcgaa cgtcgacgag gccgcggcgg
     4861 caggtgagag cgtggtcggg ctgctgtcgt tcctccaggc gtggctggcc gcggatgcgt
     4921 tgagcgagtc gcgcctgacg ctcgtcacgc gcggcgcgat gggcgtcagg gacggcgagg
     4981 cgcccgatcc ggctcgctcc gcgtggtggg gcctcgcgcg cagcgcccag tccgagcacc
     5041 ccgggcggct cgtcctcgtg gacctcgacg agcacgccga tacgcgcgag gtcgactggt
     5101 ccgcgctgct gggggccgac gagccgcagc tggcgttgcg gcgcggcggc gtgtacgggt
     5161 tccgcctcgt ccgtctccag acgagcctcg ccctggtccc gccggctggt gaggacgctt
     5221 ggcaactcgc cacgccgcgg cacgggacgc tcgaggatct cgccctcgtc gagagcgagc
     5281 gcgcgaagag ggcgctggaa ccggacgagg tccggatcgc cgtacgcgcg gccggcgtca
     5341 acttccgcga cgtgctgatc gccatcgggc aggtcgacga ccccgagccg atcggcggcg
     5401 agggggccgg cgtcgtgctc gaggtggggg acgcggtgac ggacctcgcg gtcggcgacc
     5461 gggtcgtggg tgtgatgccc caatcgctcg gacccgtcgc ggtgagcgac cgccgggtcc
     5521 tcgccaagct gccggaggac tggtcgttcg tcgagggggc gtcggtcccc atcgccttca
     5581 tgacggctta ctacggcctg ctcgagctcg gcgagctcgg cgagggacag cgggtcctcg
     5641 tgcacgcggg cgcgggaggc gtcgggatgg ccgcgatcca gatggcgcag gaccgcggcg
     5701 ccgaggtcta cgccacggcc agccccgcga agtgggacgt cctccgcggc ctggggctcg
     5761 acgacgacca catcgcctcg tcccgcgacc tcgacttccg cgaccgcttc ctggcggcga
     5821 cggacggcga gggcgtcgac gtggtgctga acgcgctcgc gcgtgagttc gtcgacgcca
     5881 ccctcgagct cctcccgcgc ggtggccgat tcgtcgagat gggcaagacc gacatccgcg
     5941 acgccgacca ggtccggcgt gaccatccgg gcgtcgagta ccgcgtattc gacatcatcc
     6001 gcgacatcag ctcggagtac gccgggaagg ttctcggcgg cgtcatggag ctgttcgagc
     6061 gcggcgcgct gcgccacctg ccgatccgcg catgggacgt ccgccacgct gcctcggcct
     6121 tccgccacct cgccgagggg cgcaacgtcg gcaaggtcgt gctgaccatc gcgcaatcgc
     6181 tcgatccgga gggcacggtc ctgatcactg gcggcaccgg cgatctcggc gggcgcgtgg
     6241 cgcgccactt cgcgggtgag cacggcgtcc gccgcctgct gctcacgagc cgccgcgggc
     6301 cggacgcgcc gggcgccacg gaactcgtcg aggagctgcg cgagctgggg gccgacgcga
     6361 gcgtcgtggc ctgcgacgcc gccgaccgcg acgacctctc cgccctgctt gcctcgatcg
     6421 acgccgagca tccgctgacg gcggtgatcc acaccgcggg cgtgctcgac gacgcggtga
     6481 tcgagtcgct gaccggcgag cagatcgagc gcgtgatgcg gccgaaggtc gacgcggccc
     6541 tgttgctcga cgagctgacc cgcgggagcg agctggccga gttcgtgctg ttctcctcgc
     6601 agtcgggcac gatgggcgcg ccgggccagg gcaactacgc ggcggcgaac gtcttcctgg
     6661 atgcgctcgc cgagcgccgc cgcgcgcagg ggctgccggc caagtcgctg gcctgggggc
     6721 tgtggagcga cgcacgtggg atggccggcc agctcgacga ggccgacgcc gcacgcctgg
     6781 cgcgcctcgg cgtggcggtg atgcgcgacg agctgcagct gctcgacgcc gctcgcgagg
     6841 ccgccgagtc ggtcgtcgtc cccacccggt tcgacatgtc gggcctgcgc tccgcggccg
     6901 aggcggggac cctcccgccg ctcctgcagg acctcgtccg gcggccgcag cgcgcgcgcg
     6961 agaccggccg gtcgctcgac caggagctcg ccggagtgcc cgaggacgag cgcgaggaca
     7021 gggtgctggc tgtcgtgtcg gagcaggtcg cggccgtgct cgggcacagc tcggcggagg
     7081 ccatcgaccc cgagcgcacc ttcaaggggc tcggtttcga ctcgctcggc gcggtcgagc
     7141 tgcgcaaccg cctcatgcgg gtcagcgggc agcggctgcc ctcgaccctg atcttcgatc
     7201 acccgagtcc cgtggccgtg gctcagtacg tgctggagaa gctcgccctg ccgccggccc
     7261 ccgacgccga gccgagccgt gaggcgggcg atgagatcga gatccgcgag gagtccgacg
     7321 gcctcgacga gctggacgcg gacgagctga tccgcatggc gaaggagagc tgatgagccc
     7381 ctctcacgaa gagctcaccg atgcgctccg cagctcgatc agggatcgcg agcagctgcg
     7441 cgaggagaac cggcggctgg ccgcgaagga ggccgagccg atcgccgtgg tcggcatggc
     7501 ctgccggttc cccggcggcg tgcgatcgcc ggacgacctg tggcgggtcc tcgtctcggg
     7561 aaccgacacg atctcggagt tcccgaccga tcgaggctgg gatctcgacc gcatctacga
     7621 ccccgacccg gacgcgcgcg ggaaggtcta ctcacgttac gccggtttcg cggacgacgt
     7681 caccgacttc gacgccgact tcttcgagat cagtcccaac gaggcgctcg cgatggaccc
     7741 gcagcagcgc gtgctgctgg aggtcatgtg ggaggcgatc gagaacgccg gcatcgatcc
     7801 agcatcgctg acgcgaacgc ccagcggtgt cttcgtcggc gccagcgcgc ccgactacgc
     7861 ggggcggctc gcggccgagg tcgagggcta ccgcatgacg ggctcgctcc cgagcgtcat
     7921 atccgggcgc gtcgcgtacg agctcggcct cgagggcccg gcggtgaccg tcgacaccgc
     7981 ctgctcgtcg tcgctcgtgg ccacgcacct cgcctgccag gcactccgac gcggcgagac
     8041 atcgctcgcc ctcgccggcg gcgtgacgat cctggccacc ccctggatgc tggtggagtt
     8101 cagccgccag cgcggcctgg cacgcgacgg ccgctgcaag tcgttcgccg acagcgcgga
     8161 cgggaccggc atgtcggagg gcgcgggcgt gctcgtgctc gagcgcctgt cggacgcaca
     8221 tcgcaacggg catcgcgtgc tggcggtgat ccgcggtagc gcggtcaacc aggacggcgc
     8281 gagcaacggt ctgtccgcgc cgaacggacc gtcgcaggag cgtgtgatcc gcgacgcgct
     8341 ggccgccgcg gatctgtcgg ccgccgaggt ggacgcggtc gaggcgcacg gtacgggcac
     8401 cacgctcgga gatccgatcg aggcgcaggc gttgatcgcc acctacggcc aggagcgctc
     8461 ggacgggccg ctccggctcg ggtcgatcaa gtcgaacatc ggtcatccgc tggcggcggc
     8521 cggcgtcgcc ggggtgatga agctggtcct ggcgctgcgc cacgggctgc tgccgccgac
     8581 gctgcacgtc gacgagccct cgtcgcacat cgactggacc gaaggcgaga tcgacctcct
     8641 gaccgaggct cagccgtggc cgcgcggcga gcgcccacgg cgggcgggcg tgtcgtcctt
     8701 cgggatcagc gggaccaacg cccacgcgat catcgaggag gcgcccgagc tggaggagcc
     8761 ggaggcggcg tcctccgagg cgaccgagcc gccggtgatg gcgtggctcg tgtcggccaa
     8821 gagcgaggag gcgctccggg cccaggcgcg cgagctgcgc gcgcacgtgg aggagcggcc
     8881 cgagctgtcg ccggtggaca tcgcgttcac gctggccacc ggccgcgcgc ggctcctcaa
     8941 gagtgccgtc gcgatcggcg cggaccgcga cgcgctgctg gccgggctcg acgccatcga
     9001 gcgcggcgag cggccggcgg gcgagctgcg tcgcggcaag accgcgttcc tgttcaccgg
     9061 acagggggcg cagcgggcgg ggatgggccg cgagctcgcc gaggcgttcc cggcgttcgc
     9121 ggaggccctc gacgaggtct gcgcggagct cgatcgccac ggcgaccgcc cgctcaagga
     9181 gctgctgttc gcggagcccg gctccgatgc cgccgcgctg ctcgaccgga ccgagtacac
     9241 ccagaccgcg ctgttcgccg tcgaggtcgc gctattccgc ctggtcgagt cgctcgggat
     9301 gaagcccgac tacctcgccg ggcactcgat cggcgagctg accgcggccc acgtgtccgg
     9361 ggtgctctcg ctccccgacg cctgcgccct ggtggccgcc cgcgggcggc tgatgggcgc
     9421 gctgcccgac ggcggcggga tgctcgcgat cagggccgcc gaggacgagg tggcggaatc
     9481 gctcgagggc ttcgagggcc ggctgtcgat cgccgccgtc aacgggccgc gggcgatcgt
     9541 cgtctccggc gaggcggagg cgctcgacga gctcgagccg gtgtggaagg agcgcggggc
     9601 cgacaccaag cgcctgcgcg tgagccacgc cttccactcg ccgctgatgg agccgatgct
     9661 cgacgagttc cgcaaggtgg ccgaggggcg gcgcttctcg cggccgcgga tcccgatcgt
     9721 gtccgccctc accggcgagc gcatcgcttc ggagaagatc tccgaccccg acttctgggt
     9781 ccgtcacgtg cgcgagccgg tccgcttcgc cgacgccgtg acggcgctcc acggcgccgg
     9841 cgtcacgcgc ttcctcgagc tcgggccgga cggggtcctg acggcgatgg ccggcgagag
     9901 cctcgacccg gacgaccggg agcgcgcggt gctcgtaccc gcgctgcgct cgaagcgcgg
     9961 cgacggcgag acgttcgtcg ccttcctcgc cgcggctcac gccgccggcg ccgcggtgga
    10021 ctggagggcg ttcttcgacg gcagcggcgc ccggcccgtc gatcttccga cctatgcgtt
    10081 ccagcgcaag cgctactggc tcgtgccggc ggccggcgcg ggcgatctcg ccgccgccgg
    10141 ccagctcgcg gccgggcacc cgctgctcgg cgcgacggtg cggctcgccg gcgagcggga
    10201 cggctggctc ctcaccggcc gcctgtcgct cgagacgcac ccgtggctcg cggaccacgc
    10261 ggtcatggac accgtgctcc ttccgggcac cgcgttcgtc gagctggcgc tcgccgccgc
    10321 gcgccgcgtc ggcgcgggag ggatcgagga gctgacgctc gtcgcgccgc tcgtactcga
    10381 tggcgagcgg gcgctgcagg tggcggtggc ggagcccggc gacgacggcc gccgcgcggt
    10441 cgaggtctac tcgcgcgcgc agggtggcgc ggacgacggc gcggacgacg aggagtggac
    10501 gctgcacgcc tcgggcctcc tcggcgcggc cggtgcggag ggcgcccacg cggcgctcga
    10561 ggccttcgcc gcggcgagct ggccgccgga cggcgccgag gagctcgacg tcgaggacct
    10621 ctacgaccgc ctggccgagg ccggctacga ctacggcccc gccttccagg gcctccagag
    10681 cgcctaccgc gcgggtgagg agctgtacgc cgaggtggcg ctcgacggcg aggagggcga
    10741 gggcggcggc ttcggcgccc acccggcgct cctcgacgcg gcgcttcacg cgatggtcct
    10801 cggccgcgag gcggacgcgt cacccgaggt gcccttctcc ttctccggcg ttcgcctcga
    10861 gcacgagggc gccgcatcgc tgcgggttcg gatcgagacg agcggcgacg gcgaaaccac
    10921 caggatcggg ctccaggcgc tcgacgagac cggcgcgccg gtgctcgcga tcgacgctct
    10981 cgagatgcgc cccgtcgacc gtgcggcgct cagggcaggg gcgagcgtgg ggcgggatgg
    11041 gctcttcgcg ctcgggtggg tcgaggtcga ggccgcggac cccggtggtg cggcgcccgg
    11101 cctggcgctg atcggcgccg ccgacgagct cggctcgcag ctcgacgcga ccggctactc
    11161 cgacctggcg accctcgaaa gggcgatcgc cgacggggcg gaggcacccg cggtggtgct
    11221 cgcccgcgcc ggcgcagccg acgggccgct cgccgggtca gtccacgagc tcgccgagcg
    11281 gacgctcgag ctgctgcagg actggctcga ctcggaggcg ctggccgacg cccggctcgt
    11341 gctgctcacc gaccgcgccc tggccgtcac cgagggcgag gccccgaacc tggcccacgc
    11401 cgccctggcc ggactcctgc gcagcgcgca gtcggagaac ccggggcgct tcgccctggt
    11461 cgacctcgac ggaagcgacg cctccgcgac cggcctccac gcggcgctgg ccagcgagga
    11521 gccggagctc gcgctcaggg acggcacgct ccatgcgccg cgcctttccc gctccggcgc
    11581 gctcgccgta ccggccggcg gctccgcctg gcggctcgcg atcgagcgca agggctcgct
    11641 cgaggacctc gcgctcgtgg cctccgaggc cgccgaggct ccgctgggcg agggccaggt
    11701 ccgcgtggcc atgcgcgccg ccggactcaa cttccgcgac gtgctgatcg ccctcgggat
    11761 gtaccccggc gaggccccgc tcggcagcga gggagcggga gtggtcgtcg aggtcggacc
    11821 gggggtggag cgattcgcgc cgggcgacag ggtgatgggc ttcgtcgagg aggcattcgg
    11881 ctcgcacgcg gtcggcgacg agcgcacgct cgtctcgatc ccggaggcct ggtcctacac
    11941 cgaggcggcc gccgtcccga tcgcattcct gaccgcgtac tacggg
//
LOCUS       DASVPL010000319         9111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_349359, whole genome shotgun sequence.
ACCESSION   DASVPL010000319 DASVPL010000000
VERSION     DASVPL010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349359"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VF517_18270"
     CDS             <1..906
                     /locus_tag="VF517_18270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8054935.1"
                     /translation="RAGADRSRAAAAPPVTGAALALVIPSAAAHAGWNLLAKQSGGGL
                     PYIWLGGVLSLGVLIPASAIATLAAGEELGLSATAAAWMLGAAAIHGTYFFTLQSGYR
                     AADLSVVYPLARGSGPLLSFIAAILLLGESPGVLQVLGAATIVGAVLALGLSAARRAP
                     HDRSGIAWALTTGGLIATYTLWDKHGVDGLDVQPIAYLCGVEIGRALIFLPLVMRRRD
                     ELAAEWRHKRRNLLGFAAMAPLSYVLFLFALSLAPVTLVAPLRELSILFGVALGGQFL
                     AEGHTRTRALAAVAIVAGVSAVSIG"
     gene            923..1306
                     /locus_tag="VF517_18275"
     CDS             923..1306
                     /locus_tag="VF517_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054936.1"
                     /translation="MPLEPGISRTDEFTVEGPLLTDVGGTIGVSVLSTPGMIAMMERN
                     AAVLAFENLPEGKATVGFEVCVKHVAGAGEGSVCTASAVLREVIDGRKLRFDVEVKEG
                     ERTIGVGTHERRAIDVAAHAESTGA"
     gene            1299..3230
                     /locus_tag="VF517_18280"
     CDS             1299..3230
                     /locus_tag="VF517_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054937.1"
                     /translation="MLRAALIAALLAAVLAPVASAVPRDTTVRTIVDRDGDNRLEFGP
                     GEDYTYQGDGAPPRVEGSILNFLQLSDFQVVDEESPGRVEFLDETQQPPLNPVGAAYR
                     PQEALSPFFVEAMVRQARNSVSPLTHEQLELALLTGDNADSQQYNETRWFIDMLDGQK
                     KIDPNSGIEGVCDPTPGQLYDGVRDGGREQGYYEPDASAGGEDGRGYTPNRQENQAET
                     GRDVTVRDFPGLLEAAQHRFESVGLDMPWYSAFGNHDALIQGNSPEAYIGPDGPSGEQ
                     SNDAFQRLVTGCVKPSKLPPGVAADDIASIFGDPAAAAPLTVPPDFRRCYLAKDASAP
                     LFGGAPPPCGASPLVPGPLGAPPGFENTFTPTGGWIQQHFITTGTPVGHGFSNRPPTA
                     VANHDGYYSFSPRPGLRFAVLDTVTDDCGAGVQVCAEGSIDDQQFEWLRGEIEAAAAA
                     GQYVITFSHHTMRTTRSPDGDATEQPVHYGEVADRRDGQPLPPSPSETLEQLHCEHPN
                     VLAHVAGHEHQNFVEHHECAADQPPTPGGGDFWEISTAAHLDWPQQARMIELIDNPNG
                     RMSLALTMLDHDGPPEPGGAPPDPNANGASGEQVLKLASIGRELAYNDYQASRAANGT
                     PADRNTILPLAQPWPYGSD"
     gene            complement(3227..3961)
                     /locus_tag="VF517_18285"
     CDS             complement(3227..3961)
                     /locus_tag="VF517_18285"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEX8054938.1"
                     /translation="MHRALGATAIAIVAIGLLAAGPSPAWADLPAGRHVLLGHSTEGR
                     AIRAVRVGDTGSPRKALVVGAIHGNELAGLRVTRALRELGGGADVDLWVVDTVNPDGR
                     AHGTRRNARGVDLNRNFPRRWRRSSRGSSYYSGSRRLSEQESRIVVRWVERLRPAVTI
                     WYHQPWGAVLLPCSGPAPIERHYMRLARFPGERCRGAGLFGTATSWQNHALPGTRAFV
                     VELPAAGITARDAARHARAALGVAAG"
     gene            complement(3954..4439)
                     /locus_tag="VF517_18290"
     CDS             complement(3954..4439)
                     /locus_tag="VF517_18290"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX8054939.1"
                     /translation="MSLPAWQRPLVKIASSRGGSWYFLNVATPIDRRLIPATRGRLSS
                     APGKPVCVVETVGARSGQPRRIPLLYAHEGDDLILIASSTGAKKNPAWFHNIRKNPSV
                     RVWAPRGRSGEYVARVAEGAERERLWNRANELYPGYETYDVRSGSREIPVVALSPRRS
                     A"
     gene            complement(4436..6040)
                     /locus_tag="VF517_18295"
     CDS             complement(4436..6040)
                     /locus_tag="VF517_18295"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX8054940.1"
                     /translation="MGIRGVLALAAALALAVAAPAGAAEWLAGDGHVHTCYSHDAYCG
                     PGDDEGQDAFYSFGGSVTERFAEAALKGMDFLVISDHDDIRAHTDPAFGSQGVTGIHA
                     YEASLSGGHAQMIGARKTYPKGDGLAPATIAMADLLRADGGIFQANHPGYRLTGASIS
                     SCAEAAGTRMHWQYGYSVLPDTIEVWNATSLLRPAELFWECWLQRGARISALAGSDSH
                     GGNHVNIGLPTTWAFAASKGEADVLAALRAGRTTLSRVAPNQGAVRLILEGDKDGDGR
                     FEAMIGDEVPPRTPMRVRTEGAPLAGLLSVRANGKPVESDTALPPGGQRVFRAPAEPG
                     WVRATLYLQDGVSDVDPSCAVPEPFPSPPLSTCSEDLAVAAMTSPIWVGEAAAPGQQP
                     PPPPPPRVDPPPATPPQERPESQEPDRQEPVPPAQQGGREPMPDVPPQGERPPPVSKL
                     RATWFRAAAARHRSVRVRLRWKASAGPFDVQVRRPGARKWRKIARRTQKRQVIVRLAR
                     GRWELRARAVPAFAPAGPWRSLKVRL"
     gene            complement(6083..6934)
                     /locus_tag="VF517_18300"
     CDS             complement(6083..6934)
                     /locus_tag="VF517_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8054941.1"
                     /translation="MEVGIGLPNAVQGVTGKQLVDFGRAADEAGFPSLGALDRVVYHS
                     YEPLVTFSAIAAVTERIRLTTAVLLAPLRSTAILAKQAAAVHDISGGRLVLGMGLGAR
                     EDDYGTSEIDTSPKGKRFSGQLDELKRLWGGEERGHAGAVGPTLPTPPSVIVGGGVEA
                     SFKRAAKYGDGWIMGGGTPDQFREGAAATREAWRAEGREGEPRLMALAYYSLGANSDQ
                     GVENVRHYYDWLGEYGDQIASSVAADEETVKGYVAAFADAGCDELLLFAGTPDLDQVS
                     LLREAVG"
     gene            6984..7412
                     /locus_tag="VF517_18305"
     CDS             6984..7412
                     /locus_tag="VF517_18305"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX8054942.1"
                     /translation="MTPAGLEDVPGWARELIEHAPVAHLGLIDGRARPRVLPVTFALC
                     GEDLVSAVDHKPKRVEGRELARVRWLRREPSAALTVDRYDDDWSRLAWVQALGRAEVL
                     DKAPPGALDALGAKYEQYRERPPAGPFIRLVPDRFVCWTA"
     gene            7493..>9111
                     /locus_tag="VF517_18310"
     CDS             7493..>9111
                     /locus_tag="VF517_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054943.1"
                     /translation="MKLAFLVMAVAVAVTGALTAAAPAGAADRKLLSEFQDDRVFTRE
                     GPDRMRKALDELQLMGVDMIRTVVNWNRLAPASLQKDVPQGFDLTDPAAYSRQDWDAF
                     DDLVREATARGIQVHMNPAGHTPEWASRCEVNLYRACLPDPQLFQQFVTAVARRYDGT
                     YVDETDGQVLPRVSTWSLWNEPNINSWVNPQTVGSGKRQRRTAARIYRDLFHAGHDAL
                     KATGHGSDTVFLGETAPIGAPPKRTSPIQFYRDLFCIDSRGRKLRGQAAKKLGCTKPR
                     PFKVTGISHHPYVKGAGPPLPAKLVEGAASVGTLTALEKVLDQGARRGMIRRKAPIYI
                     TEFGISTNPPDTKYGVPLERAAAHLNQADRYMWLDKRVRMVSQFEYEDDVALNPPTFQ
                     TGLRYGSTGLPKPSLGAYRLPVFVLPDPKKSKTRAIVWGWVRAVKNQRQQVVIQNRRE
                     TYGEWSNVRTVTTDAYGFLNVRVERLKGSWRLAWVHPSGEIVTSRVARVDRFDRAPSP
                     GFSPGDQDVPAPGGGDAGGAGGGGTGGGGTGT"
BASE COUNT         1237 a         3471 c         3210 g         1193 t
ORIGIN      
        1 cgcgccgggg cggaccggag ccgcgccgcg gcggccccgc ccgtgaccgg cgcggccctt
       61 gccctcgtca tcccgagcgc tgccgctcac gccggctgga acctgctcgc caagcagtcc
      121 ggcggtgggc ttccgtacat ctggctcggg ggagtgctca gcctcggcgt cctgatcccg
      181 gcctccgcca tcgccacgct cgcggccggc gaggagctcg gcctcagtgc cacggcggcg
      241 gcctggatgc tcggcgccgc cgccatccac ggcacctact tcttcaccct ccagagcggc
      301 taccgcgccg ccgacctgtc ggtcgtctac ccgctcgcgc gcggcagcgg cccgctgctc
      361 tcgttcatcg cggcgatcct gctgctcggc gaatcgcccg gcgtgctcca ggtgctcggc
      421 gccgcgacga tcgtcggcgc cgtgctggcg ctcggcctat cggccgcgcg gcgcgccccg
      481 cacgaccgct ccggcatcgc gtgggcgctc acgaccggcg ggctcatcgc gacgtacacg
      541 ctgtgggaca agcacggcgt cgacggcctc gacgttcagc cgatcgcgta cctctgcggt
      601 gtggagatcg ggcgcgcgct gatcttcctg ccgctcgtga tgcgccgccg cgacgagctg
      661 gccgccgagt ggcgccacaa gcgccgcaac ctcctcggct tcgccgcgat ggcgcccctc
      721 tcgtacgtgc tgttcctgtt cgcgctgtcg ctcgctcccg taacgctggt cgcgcccctg
      781 cgcgagctga gcatcctgtt cggcgtggct ctaggcgggc agttcctggc cgagggacac
      841 acccgaacgc gcgcgctcgc ggccgtggcg atcgtcgcgg gcgtctcggc cgtctcgatc
      901 ggctgacgcg tacgatgcgc gcgtgccact cgagcccggc atctcgcgca ccgacgagtt
      961 caccgtcgag gggccgctgc tgacggacgt gggcggcacg atcggcgtca gcgtgctctc
     1021 gacgccgggc atgatcgcga tgatggagcg caacgccgcg gtcctcgcct tcgagaacct
     1081 ccccgagggc aaggccaccg tcggcttcga ggtctgcgtg aagcacgtgg cgggtgcggg
     1141 ggagggctcg gtctgcaccg cgagcgcggt gctgcgcgag gtgatcgacg ggcgtaagct
     1201 gcgcttcgat gtggaggtca aggagggtga gcggacgatc ggggtcggca cgcacgagcg
     1261 ccgcgcgatc gacgtcgccg cccatgccga gagcacgggt gcttagagcc gcgctgatcg
     1321 cggcgctgct cgccgcggtc ctcgctcccg tggcgtcggc cgtgccgcgc gacacgacgg
     1381 tccgcacgat cgtcgaccgt gacggcgaca accgcctcga gttcggcccc ggcgaggact
     1441 acacctacca gggcgacggc gccccgcccc gggtcgaggg ttcgatcctc aacttcctcc
     1501 agctctccga cttccaggtc gtggacgagg agtcgccggg ccgggtcgag ttcctcgacg
     1561 agacccagca gccgccgctc aacccggtcg gggccgcgta ccggccgcag gaggcgctca
     1621 gcccgttctt cgtagaggcg atggtgcgcc aggcgcgcaa ctcggtctcg ccgctgaccc
     1681 acgagcagct cgagctcgcg ctcctcaccg gcgacaacgc cgactcgcag cagtacaacg
     1741 agacgcgctg gttcatcgac atgctcgacg gccagaagaa gatcgacccg aactcgggca
     1801 tcgagggcgt gtgcgacccg acccccggcc agctctacga cggtgtgcgc gacggcggtc
     1861 gggagcaggg ctactacgag cccgacgcgt cggcgggtgg cgaggacggg cgcggctaca
     1921 cgcccaaccg ccaggagaac caggccgaga ccggccgcga cgtgaccgtc cgcgacttcc
     1981 ccgggctgct cgaggccgcg cagcaccggt tcgagtcggt cggcctcgac atgccgtggt
     2041 actcggcgtt cggcaaccac gacgccctca tccagggcaa cagccccgag gcatacatcg
     2101 ggccggacgg tccgagcggc gagcagtcga acgacgcgtt ccagcgactc gtgaccggct
     2161 gcgtgaagcc ctcgaagctg ccgcccggcg tcgcggccga cgacatcgcg agcatcttcg
     2221 gcgaccccgc cgccgcggcg ccgctcaccg tcccgcccga cttccgccgc tgctacctcg
     2281 cgaaggacgc gagcgcgccc ctgttcggag gcgcgccgcc gccgtgcggc gcgagcccgc
     2341 tggttcccgg tccgctcggc gccccaccgg ggttcgagaa cacgttcacg cccacgggcg
     2401 ggtggatcca gcagcacttc atcacgaccg gtacaccggt cggccacggc ttctccaacc
     2461 gcccgccgac ggccgtggcc aaccacgacg gctactactc cttctcgccg cgccccggcc
     2521 tgcgcttcgc cgtgctcgac acggtcaccg acgactgcgg tgccggagtg caggtctgcg
     2581 ccgagggatc gatcgacgac cagcagttcg agtggctgcg cggtgagatc gaggcggccg
     2641 cggccgcggg tcagtacgtg atcacgttct cccaccacac catgcgcacg actcgttccc
     2701 ccgacggcga cgcgaccgag cagccggtgc attacggcga ggtcgccgac cgccgcgacg
     2761 gccagccgct gccgccctcc cccagcgaga cgctcgagca gctccactgc gagcacccga
     2821 acgtgctcgc gcacgtggcg ggccacgagc accagaactt cgtcgagcac cacgagtgcg
     2881 ccgccgacca gcctccgacc cccggcggcg gcgacttctg ggagatctcg acggccgccc
     2941 acctcgactg gccgcagcag gcgcggatga tcgagctgat cgacaacccg aacggccgca
     3001 tgtcgctcgc cctcacgatg ctcgaccacg acggcccgcc cgagccgggc ggggcgccgc
     3061 cggacccgaa cgcgaacggt gcctccgggg agcaggtgct gaagctcgcg tcgatcggcc
     3121 gcgagctcgc ctacaacgac taccaggcca gccgcgccgc caacggcacg ccggcggacc
     3181 gcaacacgat cctcccgctc gcccagccct ggccgtacgg ctcggactag ccggcggcca
     3241 cgcccaaggc cgcgcgcgcg tggcgcgcgg cgtcccgcgc cgtgatcccc gcggccggta
     3301 gctccaccac gaacgcccgc gtgcccggca gagcgtggtt ctgccagctc gtggccgtgc
     3361 cgaacagccc ggcgccgcgg cagcgctcgc ccgggaagcg agcgagccgc atgtagtggc
     3421 gctcgatcgg cgcggggccc gagcaaggca gcaggacggc gccccacggc tggtggtacc
     3481 agatcgtgac cgccggccgc agccgctcga cccagcgcac cacgatccgc gactcctgct
     3541 ccgacaggcg ccggctgccc gagtaatagg agctcccgcg ggagctgcgg cgccagcggc
     3601 gcgggaagtt gcggttgagg tccaccccgc gcgcgttgcg acgggtgccg tgcgcgcgcc
     3661 cgtccgggtt gaccgtgtcg accacccaca ggtccacgtc cgcgccgccg cccagctcgc
     3721 gcagcgcgcg cgtgactcgc aggcccgcca gctcgttgcc gtggatggcg ccgaccacca
     3781 gcgccttgcg cggggagccg gtgtcgccca cccgcacggc gcggatggcg cggccctcgg
     3841 tggagtgccc gagcagcacg tgccggccgg ccgggaggtc cgcccaggcc gggctcggtc
     3901 cagccgcgag caggccgatc gccacgatgg cgatcgccgt cgcgccgagg gctctatgca
     3961 ctccgccgcg gcgacaacgc aaccaccggg atctcccgcg agccggacct gacgtcgtag
     4021 gtctcgtagc ccgggtagag ctcgttcgcg cgattccaga ggcgctcgcg ctcggcgccc
     4081 tcggccacgc gcgcgacgta ctcgccgctg cggccgcgcg gcgcccacac gcgcacgctc
     4141 gggttcttgc ggatgttgtg aaaccaggcc gggttcttct tcgcgccggt ggacgacgcg
     4201 atcaggatca ggtcgtcgcc ctcgtgcgcg tagagcagag ggatgcgccg cggctgcccg
     4261 ctcctcgccc cgacggtctc gacgacgcac accggcttgc cgggcgcgct gctcaaccgc
     4321 ccgcgcgtgg ccgggatcag gcggcggtcg atcggggtcg ccacgttcag gaagtaccac
     4381 gacccaccgc gcgaggacgc gatcttcacg agcggccgct gccaggccgg caggctcaca
     4441 ggcgcacctt caaggagcgc cacgggcccg cgggcgcgaa cgccggcacc gcgcgagcgc
     4501 gcagctccca gcggccgcgc gcgagccgca cgatcacctg ccgcttctgc gtgcgtcgcg
     4561 cgatcttccg ccacttccgc gcgccggggc ggcgcacctg cacgtcgaac ggccccgcgc
     4621 tcgccttcca gcgcaggcgc acgcgcaccg agcgatgccg ggcggcggcg gcgcggaacc
     4681 acgtggcgcg cagcttcgac acgggcggcg ggcgctcgcc ctgcggcggc acgtcgggca
     4741 tcggctcgcg gccgccctgc tgcgccggcg gcaccggctc ctggcggtcc ggctcctgcg
     4801 actcgggccg ctcctgcggc ggcgtggccg gcggcgggtc cacccgcggg ggcggcggcg
     4861 ggggcggctg ctgaccggga gcggcggcct cgccgaccca gatcggcgag gtcatcgccg
     4921 ccaccgcgag atcctcggag caggtcgaga gcggcggcga agggaacggc tccgggaccg
     4981 cgcagctcgg gtccacgtcg ctcacgccgt cctggaggta gagcgtggcc cgtacccagc
     5041 ccggctcggc cggggccctg aacacgcgct ggccgccggg cggcagcgcg gtgtcgctct
     5101 cgaccggctt gccgttggcg cggacgctca ggagccccgc gagcggcgcg ccctcggtcc
     5161 gcacgcgcat cggcgtccgc ggcggcacct cgtctccgat catcgcctcg aacctgccgt
     5221 cgccgtcctt gtcgccctcg aggatcagtc gcaccgcgcc ctggttggga gctacccgcg
     5281 acagggtggt gcgtcccgcg cgcagcgccg ccagcacgtc ggcctcgccc ttcgaggccg
     5341 cgaacgccca cgtggtcggc agcccgatgt tcacgtggtt gccgccgtgc gagtcgctgc
     5401 ccgcgagcgc ggagatgcgc gcgccgcgct ggagccagca ctcccagaag agctcggccg
     5461 ggcgcagcag cgaggtcgcg ttccagacct cgatcgtgtc cggcagcacc gagtagccgt
     5521 actgccagtg catgcgggtg ccggcggcct ccgcgcacga cgagatcgac gcgccggtga
     5581 gccggtagcc ggggtggttg gcctggaata tgccgccgtc ggcgcgcagc aggtccgcca
     5641 tcgcgatcgt cgccggcgcg agcccgtcgc ccttcggata ggtcttgcgc gcgccgatca
     5701 tctgcgcgtg gccgccgctc agcgacgcct cgtacgcgtg gatgccggtg accccctgcg
     5761 agccgaaggc cgggtccgtg tgcgcgcgga tgtcgtcgtg gtcgctgatc acgaggaagt
     5821 ccatcccctt cagagcggcc tcggcgaaac gctccgtcac gctcccgccg aacgagtaga
     5881 acgcgtcctg cccctcgtcg tcgccgggac cgcagtaggc gtcgtgcgag tagcacgtgt
     5941 gcacgtgccc gtcgcccgcg agccactcgg ccgcgccggc cggtgccgcc accgccagtg
     6001 ccagcgccgc agccagcgca agaacccccc ggatccccat cacgcgggag gatggcacgc
     6061 gcgcgcccgc gtgtcgccgg ccctaaccga cagcctcgcg cagcagcgac acctgatcga
     6121 ggtccggcgt gcccgcgaac agcagcagct cgtcgcagcc ggcgtccgcg aaggcggcga
     6181 cgtagccctt caccgtctcc tcgtcggccg ccacgctgga cgcgatctgg tcgccgtact
     6241 cgccgagcca gtcgtagtag tggcgaacgt tctcgacgcc ctggtcggag ttcgcgccga
     6301 gcgagtagta ggccaacgcc atcagccgcg gctcgccctc gcgcccctcc gcccgccagg
     6361 cctcgcgcgt ggcggccgcg ccctcgcgga actggtcggg cgtgccgccg cccatgatcc
     6421 agccgtcgcc gtacttggcg gcgcgcttga acgaggcctc cacgccgccg ccgacgatca
     6481 cgctcggggg cgtggggagc gtcggcccga ccgcaccggc gtggccgcgc tcctctcccc
     6541 cccagagccg cttcagctcg tcgagttgcc cactgaagcg cttgcccttc gggctggtgt
     6601 cgatctcgct ggtcccgtag tcgtcctcgc gcgcgccgag gcccatgccg agcacgagcc
     6661 gcccgccgga gatgtcgtgc accgcggcgg cctgcttggc gaggatcgcg gtgctgcgca
     6721 gcggagcgag caggacggcg gtggtcaggc ggatccgctc ggttacggcg gcgatcgccg
     6781 agaaggtcac cagcggctcg tagctgtggt agacgacccg gtcgagcgcg ccgagcgacg
     6841 ggaagcccgc ttcgtcggcg gcgcggccga agtccaccag ctgcttgccg gtcacgccct
     6901 gcacggcgtt cggcagtccg attcccactt ccatttcagt tctcccttgg cgtcgagtcg
     6961 cggcggcgta tgctcgcaga gcgatgacgc ccgccggtct cgaagacgta cccgggtggg
     7021 cgcgggagct aatcgagcac gcgccggtgg cgcacctggg actgatcgac gggcgcgcac
     7081 gcccgcgcgt gctgccggtc acgttcgcgc tctgcggcga ggacctcgtg agcgccgtgg
     7141 accacaagcc caagcgggtg gagggacggg agctggcccg cgtccgctgg ctgcggcgcg
     7201 agccgtccgc cgcgctgacg gtggaccgct acgacgacga ctggtcgcgg ctggcctggg
     7261 ttcaggcgct ggggcgcgcg gaggtgctgg acaaggcgcc gcccggcgcg ctcgacgccc
     7321 tgggcgcgaa gtacgagcag taccgcgagc gcccgccggc cgggccgttc atccggctcg
     7381 tgccggaccg cttcgtgtgc tggaccgcgt gaccggcgtc tccgcttaac aatccgtcag
     7441 ttactttggc gccgcggcgg ggaatccgcc gcgtacccct atgcaccgca ggttgaagct
     7501 cgcattcctc gtgatggccg tcgccgtcgc ggtcaccggc gcgctcaccg ccgccgcgcc
     7561 ggccggggcc gccgaccgca agctgctctc cgagttccag gacgaccgcg tgttcacgcg
     7621 cgaaggcccg gatcgcatgc gcaaggcgct cgacgagctc cagctcatgg gcgtcgacat
     7681 gatcaggacc gtcgtcaact ggaaccggct cgcgcccgcg tcgctccaga aggacgtccc
     7741 gcaggggttc gacctcaccg acccggccgc gtactcgcgg caggactggg acgcgttcga
     7801 cgacctggtc cgcgaggcca ccgcccgcgg gatccaggtc cacatgaacc ccgccggcca
     7861 cacgcccgag tgggcgtcga ggtgcgaggt caacctctac cgcgcctgcc tcccggaccc
     7921 gcagctcttc cagcagttcg tgaccgccgt cgcccgccgc tacgacggca cctacgtcga
     7981 cgagaccgac ggtcaggtgc tcccgcgcgt gagcacgtgg tcgctctgga acgagccgaa
     8041 catcaacagc tgggtcaacc cccagacggt cggcagcggc aagcgccagc gccgcaccgc
     8101 ggcgaggatc taccgcgacc tcttccacgc cgggcacgac gcgctgaagg cgaccggcca
     8161 cggctccgac acggtgttcc tcggcgagac cgcaccgatc ggcgctccgc ccaagcgcac
     8221 ctcgccgatc cagttctacc gcgacctctt ctgcatcgac tcgcgcgggc gcaagctgcg
     8281 cgggcaggcc gcgaagaagc tcggctgcac caagccgcgc ccgttcaagg tgaccgggat
     8341 cagccaccac ccgtacgtga agggcgccgg cccgccgctc cccgcgaagc tcgtcgaggg
     8401 cgcggcctcc gtgggcacgc tcaccgctct cgagaaggtg ctcgaccagg gcgcccggcg
     8461 cgggatgatc cgccgcaagg cgccgatcta catcaccgag ttcgggatct ctacgaaccc
     8521 gcccgacacc aagtacggag tcccgctcga gcgcgccgcc gcgcacctca accaggccga
     8581 ccgctacatg tggctagaca agcgcgtgcg gatggtctcg cagttcgagt acgaggacga
     8641 cgtcgcgctc aacccgccga cgttccagac cggcctgcgc tacgggtcga ccggcctgcc
     8701 gaagccgagc ctcggcgcgt accggctgcc ggtcttcgtg ctgccggatc cgaagaagag
     8761 caagacccgc gcgatcgtgt ggggctgggt gcgcgctgtc aagaaccagc gtcagcaggt
     8821 ggtgatccag aaccgccgcg agacctacgg cgagtggagc aacgtgcgca cggtgaccac
     8881 cgacgcgtac ggattcctca acgtgcgggt cgagcggctc aagggctcat ggcgcctggc
     8941 ctgggtgcat ccgagcgggg agatcgtgac gagccgcgtc gcgcgcgtgg accgcttcga
     9001 ccgcgcgccg tcgccgggct tcagcccggg cgaccaggac gtgcccgctc cgggcggtgg
     9061 cgacgcgggc ggggcgggcg gcggcggcac gggcggcggc gggaccggca c
//
LOCUS       DASVPL010000320        15294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_349678, whole genome shotgun sequence.
ACCESSION   DASVPL010000320 DASVPL010000000
VERSION     DASVPL010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15294
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349678"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1304
                     /locus_tag="VF517_18315"
     CDS             39..1304
                     /locus_tag="VF517_18315"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8054944.1"
                     /translation="MSPRTENPAGSAFLDAFGDVSAAVESGAGLPEVARATARALNAS
                     VAVVDDTASILAVAAQSPDDEHAVLSGAPGSELLLLRVADTTVGELRYRPRGEPPPQA
                     LVRMVGTLIALEVERSRAPERASEAAVAGFVSDLLERRVTDRDNIVARGTELGSDLSA
                     GASVIVTRAHPHRPQEGDWRARVLAMAERGARGVAPGALAAPVQVSVARSGQTPGAAE
                     LVVIVPAKDPDLTDRVATALQRELESNLQSFSTVIGVSRPATDPVDVHRAGFEALLAA
                     NVAEAQGRALLSFEDTGAYRLLLPAMSDDPGELERFHQETVAPLLAYDEQYETELVRT
                     LETFLDEDGSVARTSEKLFTHRHTVRYRLERVRELTGLDVSSTDGRERLGLGLKAMRV
                     LGIMPARGPAHEPGTEAGTVPRDGADRRG"
     gene            1383..1568
                     /locus_tag="VF517_18320"
     CDS             1383..1568
                     /locus_tag="VF517_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054945.1"
                     /translation="MLYAALICCDEDCTDVFEAYGSLEELETLACDCGCALQVISISE
                     YSGVPSRDRGPELLPVG"
     gene            complement(1590..1660)
                     /locus_tag="VF517_18325"
     tRNA            complement(1590..1660)
                     /locus_tag="VF517_18325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1626..1628),aa:Gln,seq:ttg)
     gene            1713..3092
                     /gene="glmU"
                     /locus_tag="VF517_18330"
     CDS             1713..3092
                     /gene="glmU"
                     /locus_tag="VF517_18330"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX8054946.1"
                     /translation="MARDLTVLIMAAGHGTRMRSSLPKVLHPVCGRPMIEWVIAAARE
                     AGADRVVCITRPGEGVAEHLDGSVEIAEQTEGEGTGAAVLAAREALAASQTAVVLSGD
                     HPLVSPELIKSLIDAHVRAEAAATLLTTEQLDPAGYGRVVRGADGSVERIVETKHTDG
                     VSPEELAIREINIGTYAFGAADLLDALDAVGEERGERYLTGVIPIIREKGKNLAAHVT
                     ADTRSAMGVNTRADLLEIERLARLDLIERHATAGVAFTAPGTVAIDAGVEIGADTTIG
                     SGVTLTGATRVGGGCTIGPQATIADSTIEEGATVLHAVLTEAHVGPNASVGPFAYLRP
                     GAKLAEGSKVGTFVEVKNSNIGPGSKVPHLSYVGDADVGEGSNLGASTITANYDGKRK
                     HRTAIGDGVKTGIHTSLVAPLTVGDGAYTGAGSVIRKDVPDGSLAYSDAKQRNIEGYN
                     PKPAEDRPE"
     gene            3089..4120
                     /locus_tag="VF517_18335"
     CDS             3089..4120
                     /locus_tag="VF517_18335"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX8054947.1"
                     /translation="MTLETQAQSTPRTSVPHGYDKRMMVVSGRASRELGLKIADKVGV
                     DPVEADLRTFSDGEVYCRYEESIRGADIFIVQSIAGNEVEGMTANDALMELLVMIDAA
                     VGASAHRVIAVAPWYGYSRQDKKSAPREPITARLVAKCLEAAGIDRLLTMDLHAGQLQ
                     GFFQKTVDHMTAMPILTQFVKDQLGDATDLVIISPDAGRVKLTRKFAQKVGAPYALLE
                     KERPAQQVAEIGYVIGEVKDKVAVIVDDIIDTGGTLSAAAQTVLDEGASAVYAVATHG
                     IFSGKAYETLGNSPLSGIVVTDTVPLRSGAPDNIKVLTCTDILADSVRRIFTDESVSE
                     IFAGENQLF"
     gene            4138..4575
                     /locus_tag="VF517_18340"
     CDS             4138..4575
                     /locus_tag="VF517_18340"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX8054948.1"
                     /translation="MTLHILAAIIWLGGSWMLLILGLTLRGADLQRRIDFTRMTEKVP
                     SIVFAVSSLVVILAGSWLVHEAGYDYSDTWVTLGYVGWFISFLFGVGFYGPEGKRRDR
                     TIEAEGAAAPGVVKSLNRVLTVAAIDTAIITLVVVDMTTKPGL"
     gene            complement(4572..5999)
                     /locus_tag="VF517_18345"
     CDS             complement(4572..5999)
                     /locus_tag="VF517_18345"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX8054949.1"
                     /translation="MARDDETQIPTGRVQRTAKVGTVIGGQGARYAGTKAANLVRSKE
                     SADERLDARHLEAAERMVDALGNMKGAAMKIGQLASFIDTEFLPPEYAEMYQEKLSEL
                     RTSAPPMPWDKVKKVLDEEWEGEPLEDIFDEFDQTAAAAASIGQVHRATLCDGRKVAV
                     KIQYPGVADAVRADMQNAGMIMRLAKALAPGLDAKAAAAELKERVLEELDYEYEAQNQ
                     RSFSRGYRGHPFIYVPDVITRLSRRRVLVTEWVDGVGFDEIKALPQEEKDRFGEIVFR
                     FCFGSIYHLQHFNADAHPGNYVLMADGRVAFLDFGMTKQLDMEQIELEIAALEAVFDE
                     DPERLRVALHDLGFLNNPKKIDAEKLMEHVKTVGGWYMQDRDVRISPRYVMKVITAIS
                     DPRSEFYQLMRRENVPANELMGRRMETGLMAVLGQLEASRNWYRIGCEWWFADEPATE
                     LGREEWAFFESRGERRVRKFSARSG"
     gene            6028..6717
                     /locus_tag="VF517_18350"
     CDS             6028..6717
                     /locus_tag="VF517_18350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX8054950.1"
                     /translation="MAAQPQRPSLGVEERSDNGSRASRRLRREGLIPGIVYGGKDCSS
                     VKINARELRSALISSSAVIDLKVGDSKVVPVIVKDTQLHPVRDEIMHIDLLEVDLTEK
                     IQTVVPLELEGAEEAPGAKEGGVIEHVTRELNIESLPTSIPERIVADVSHMEAAATMH
                     LSELTAPEGVVFLDDPEETIVATVTIPTEVVEPDVEEETELVGDAAADVVGDADGEAG
                     GDDSEGGGEGS"
     gene            6753..7286
                     /gene="pth"
                     /locus_tag="VF517_18355"
     CDS             6753..7286
                     /gene="pth"
                     /locus_tag="VF517_18355"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX8054951.1"
                     /translation="MIVGLGNPGSKYEGTRHNVGFEVANLLAQRWGLSRAKKKYVGLY
                     TDGRTGPGGPRVGVLLPQTYMNEAGNSVGPARGALGAELDRIVVLHDEIDLPFGRIEE
                     RLGGGLAGHNGLKSLKAGLGSPDFRRVRVGVGRPDSTDPEVVSSWVLGRFSEPRDDVR
                     DLIERAADATERLISAA"
     gene            7365..10715
                     /gene="mfd"
                     /locus_tag="VF517_18360"
     CDS             7365..10715
                     /gene="mfd"
                     /locus_tag="VF517_18360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX8054952.1"
                     /translation="MPSSLRQLLPFASDDPAFGRLTSALTPGAAERAFVSQSLRPYLI
                     AAVLDDEPERPGLIVVGDDRQARDLAADLKTFLAPRTVRLYPARGVQYESHLAPPPHL
                     VGLRVAALDALLAEPDEGGAAVVVASAAALCEKVPDPELRPHGFTLERGGLVDLDEAS
                     AQLAACGYERVDQVEERGQFAIRGDILDVYPATEERAVRCELFDVEIERLTFFSTFTQ
                     RSLEEAERIEIAPAAEIGPEHRELAEMVASDEERPDIAEILPVDRFTTFLSLLPQSAL
                     VAVAAEEEIEPALTDLWHDVTLSFHDKDAHHLYVEPREELRERTALSISALAGDQPHQ
                     FRAQGADTSAKNLREAEPELEKLVRSGYRAVVAWARKGEAERAAYNLARTRANFLDGQ
                     PPEPGLTFAVASLREGFLSPQLKLAVLPEHRLLRRKRGERRSGPGAGSTAIASFTDLR
                     TGDAVVHEDHGIARFSGFETKTVGGVTRDYLELEYRGGDRVFVPSDQLFKISRYVGAE
                     ASDPPLSKLGGKQWEQMKMRARRAAQALAGELINLYAERKRRAGYGFPQDDEWQLQFE
                     AAFPYQETPDQLEAIEQVKADMEEARPMDRLICGDVGYGKTEVALRAAFKSAGDGKQV
                     MFLVPTTVLAQQHFGTFTERLRDYPFTIEVASRFRSGKETGDAVKGFQEGKVDILIGT
                     HRLLSRDVRPKDLGLLIVDEEQRFGVKQKELLRQLRLRVDVLSLSATPIPRTLQMSLA
                     GLRDISVIETPPEGRRAVRTYVGEYDEDLVREGIDREIARGGQVYFLHNRVETIDETA
                     ERVRAMCPNARVRVAHGQMAEGELEKVMLEFLRGGADVLVCTTIVEAGLDVPTANTLI
                     VERADQLGLAQLYQIRGRVGRSRERAYAYLLYPSSAVLTEQAAARLATLSDYTELGSG
                     FKIAMRDLEIRGAGNLLGDEQSGHVAAVGFELYMGMLDQAVGQLGGDASEEAPEPVRV
                     DVPVDAYVPGDYVPYEAAKIEVHRRVAGAREIAELILLRDELEDRFGPVPEPLDNLIK
                     LQDARIKLGRAGAKDVGFAGGKLRVSPVELASTAVKELREALPGAVYESGRSTVAVRM
                     PDEAAERFAALVTAAEHILRVATEPPTD"
     gene            10809..12158
                     /locus_tag="VF517_18365"
     CDS             10809..12158
                     /locus_tag="VF517_18365"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8054953.1"
                     /translation="MLRTLRLTALLAAIAAVALVATGCGNDVPAGAVAKVGDKEIKKS
                     EFNRWLQAAARQQAQSQPGQAAAPVSVPDPPNFTKCIDAKRKSTPPAQGAAPPNNEQL
                     KAQCKQEFDGLKEQTMQFLISAEWLQQEAKKRGVDASPAEVQKTFQEQKKQAFPKEAE
                     YQKFLKESGRTEQDLLFQVKLSVLTNQLQAKVVEGKGDPKPDEIEDYYNENKKRFSTP
                     ESRDLLVVLTKDEADAKKALAELKKGKPWKDVVKKYSEDTASKAQGGKLPGVTKGTQE
                     KAFDDAIFKAPKGQVQGPVKTQFGFYVFQVEKVTPATQQTLQQATDTIKNLLKSQSQQ
                     KALSDFVEDFQKRYREETVCAEDYKTSQCDNGPKPPEQQQQQQQSQPQGAPPTKDSGQ
                     PAQPVVPGGVPGQPGAPGQVPQGQVPQGGAPPQGAQGAPPQQAPPQQAPPQQAPPGG"
     gene            12169..12930
                     /locus_tag="VF517_18370"
     CDS             12169..12930
                     /locus_tag="VF517_18370"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX8054954.1"
                     /translation="MAEADSAADPGRAAEADRAAEAVRQLDELTRRLRVECPWDREQD
                     ERSIVPHTIEEAYELADAAQRGDDSKLLDELGDVLFQVHFLSLLLEERGAGGLADVAD
                     HCRTKLIRRHPHVFGEVEVEGAAEVLRNWDQIKQGEAGREAGIFGEVPEMLPGPLYAR
                     KLLRRAGGAGHEWPPEGARERLRAAAERIEGVDATPGTEPGVILDGGRDERFDLVGDL
                     LLAAVALARELRVDPELALRAAAEDMRAEIEGGGR"
     gene            12927..14201
                     /gene="eno"
                     /locus_tag="VF517_18375"
     CDS             12927..14201
                     /gene="eno"
                     /locus_tag="VF517_18375"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX8054955.1"
                     /translation="MSQIEQIHARQVLDSRGNPTVEVEVVLDSGASGRAIVPSGASTG
                     EFEAVELRDGGEDWGGKGVATAVGNVNGELAESLRGSDAADQQGLDQRMIDLDGTPNK
                     GRLGANAILGVSLAAAKAAANENGLTLWRYLGGEAARIVPVPMMNVLNGGAHADNRVD
                     FQEFMVVPVGASSFSAGLKTGVEVFHALKRTLHDRGLSTAVGDEGGFAPDLDSNEAAL
                     GMLIDGIEAAGYRPGDDVAIALDPATSELHDAGVYDLEHEGRKLSSEQMADYWAGIAS
                     RYPVLSIEDGMDEEDWDGWKALTDRIGQTVQLVGDDLFVTNTERLRRGIDSGTGNSIL
                     IKVNQIGTLTETLDAVRTAQEAGYTAVMSHRSGETEDVTIADLAVATGCGQIKTGAPS
                     RSDRTAKYNQLLRIEEALGSDAEYLGRSAFRS"
     gene            14237..14578
                     /locus_tag="VF517_18380"
     CDS             14237..14578
                     /locus_tag="VF517_18380"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX8054956.1"
                     /translation="MARAAAAPAPSRRSRTTTAPRRVGAGIHWERVGRLALLGVLGII
                     VLLYIPPVAHWFQQSSTADRSEQQLRELETERARLRSRLHELSGPGAIEREARKLGMT
                     KRDERPYVIRP"
     gene            14729..15022
                     /locus_tag="VF517_18385"
     CDS             14729..15022
                     /locus_tag="VF517_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054957.1"
                     /translation="MSYALENAIFQWEEGERHLRDAEPAERVRLQHAADRVLDRLRQR
                     LGSEFTIQELSDLYASEPDWTTDSTPPAAIDAAFARYAREAADFGGGRPRARF"
     gene            complement(15019..>15294)
                     /locus_tag="VF517_18390"
     CDS             complement(15019..>15294)
                     /locus_tag="VF517_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054958.1"
                     /translation="GDAAGGAAGGSGSGEDAGSGEDAATSDADASTSDDDAAALGGDA
                     PVGEDAAPLGEDDLPLGEDAAAGGTGEPGAEGDEAAGGGLDPDSFDA"
BASE COUNT         2309 a         5691 c         5289 g         2005 t
ORIGIN      
        1 gagctgtccc cggcgcgagc ggccctacga ttggccccgt gagcccgcgc accgagaacc
       61 ccgccggcag cgccttcctc gacgcgttcg gtgacgtctc ggccgccgtc gagtcgggag
      121 ccgggctgcc cgaggtcgcc cgcgcgaccg cccgtgcgct gaacgccagc gtggccgtgg
      181 tggacgacac cgcgagcatc ctggccgtcg ccgcccagtc gcccgacgac gagcacgccg
      241 tcctgtccgg cgcccccggg agcgagctcc tgctcctgcg cgtggccgac acgaccgtcg
      301 gcgagctgcg ctaccgcccg cgcggcgagc cgccaccgca ggcgctcgtg cgcatggtgg
      361 gcacgctgat cgcgctcgag gtcgagcgct cacgcgcgcc ggagcgcgcc agcgaggccg
      421 ccgtggccgg cttcgtgagc gacctcctcg agcgccgcgt gaccgatcgc gacaacatcg
      481 tcgcccgcgg gaccgagctc ggctccgacc tctcggcggg cgccagcgtc atcgtcaccc
      541 gcgcccaccc acaccggcca caggagggcg actggcgcgc gcgtgtgctc gccatggccg
      601 agcgcggcgc ccgcggcgtg gccccgggcg cgctcgcggc gccggtccag gtctccgttg
      661 cgcggagcgg ccagacgccc ggcgccgccg agctcgtggt gatcgtgccc gcgaaggacc
      721 ccgacctcac cgaccgggtg gccaccgccc tccagcgcga gctcgagagc aacctccagt
      781 cgttctcgac ggtgatcggc gtgagccgtc ccgccacgga cccggtggac gtgcaccgcg
      841 ccggcttcga ggcgctgctg gccgccaatg tcgccgaggc ccagggccgc gcgctgctct
      901 cgttcgagga caccggcgcc taccgccttc tgctccccgc gatgagcgac gaccccggcg
      961 agctcgagcg cttccaccag gagaccgtcg cgcccctgct cgcctacgac gagcagtacg
     1021 agaccgagct ggtccgcacg ctggagacgt tcctcgacga ggacggcagc gtcgcccgca
     1081 cctcggagaa gctcttcacc caccgccaca ccgtccgcta ccggctcgag cgtgtccgcg
     1141 agctcaccgg cctcgacgtc tcctccaccg acggccgcga gcgcctcggg ctggggctga
     1201 aggcgatgcg cgtgctcggc atcatgcccg cgcgcggtcc cgcccacgag ccgggcaccg
     1261 aggcgggcac cgtgccgcgc gacggcgcgg accgccgcgg ttagcgcgcc cgccgccccg
     1321 acgcgcggtg ccccgcaacc gatcctgcaa cccgtccggg cccgcccgca ccatcccggg
     1381 caatgctcta cgccgccctc atctgctgcg acgaggactg caccgacgtc ttcgaggcgt
     1441 acggctccct ggaggagctc gagaccctcg cgtgcgactg cggctgcgcg ctccaggtca
     1501 tctcgatctc cgagtactcc ggcgtcccat cgcgcgaccg cggccccgag ctgctcccgg
     1561 tcggctgacc ccgcgcgccg cgcccagagc tgggggggga ggaatcgaac ccccattccg
     1621 tggaccaaaa ccacgtgtct ttccgttaga cgacccccca gggccggccg tcgcgcgacg
     1681 gtaccaccgc ccggcgttag gctgatcgag gaatggcccg cgacctgacg gtcctcatca
     1741 tggcggccgg ccatggcacg cgcatgcggt cgtcgctgcc gaaggtgctg caccccgtct
     1801 gcgggcgccc gatgatcgag tgggtcatcg cggccgcgcg cgaggccggt gccgaccgcg
     1861 tcgtctgcat cacccgcccg ggcgagggcg tggccgagca tctcgacggc agcgtcgaga
     1921 tcgcggagca gaccgagggc gagggcaccg gcgccgccgt gctggccgcg cgcgaggcgc
     1981 tcgcggcgtc gcagaccgcg gtggtgctct ccggcgacca cccgctcgtc tcgcccgagc
     2041 tgatcaagag cctgatcgac gcgcacgtga gggcggaggc cgcggccacc ctcctcacca
     2101 ccgagcagct cgacccggcc ggctacggac gcgtcgtccg gggcgccgac gggagcgtcg
     2161 agcgcatcgt cgagaccaag cacaccgacg gcgtctcgcc cgaggagctc gcgatccgcg
     2221 agatcaacat cggcacctac gccttcggcg cggccgacct cctcgacgcc ctcgacgcgg
     2281 tgggggagga acgcggcgag cgctacctca ccggcgtcat cccgatcatc cgcgagaagg
     2341 gcaagaacct cgccgcgcac gtcaccgccg acacgcgctc ggcgatgggc gtcaacaccc
     2401 gggccgacct cctcgagatc gagcgcctcg cgcgcctcga cctgatcgag cgccacgcca
     2461 cggcgggcgt cgccttcacg gcgccaggca ccgtcgcgat cgacgccggg gtggagatcg
     2521 gcgccgacac caccatcggc tccggcgtga ccctcacggg cgcgacgcgc gtcggcggcg
     2581 gctgcacgat cggcccgcag gccacgatcg ccgactcgac catcgaggaa ggcgccaccg
     2641 tgctgcacgc cgtgctgacc gaggcgcacg tcggcccgaa cgccagcgtc ggcccgttcg
     2701 cctacctgcg cccgggcgcg aagctcgccg agggctcgaa ggtcggcacc ttcgtcgagg
     2761 tcaagaactc gaacatcggc cccggctcga aggtcccgca cctcagctac gtgggcgacg
     2821 ccgatgtggg cgagggcagc aacctgggcg ccagcaccat caccgcgaac tacgacggca
     2881 agcgcaagca ccgcaccgcc atcggcgacg gcgtgaagac cggcatccac acctcgctgg
     2941 ttgccccgct gaccgtcggc gacggggcgt acactggcgc tggctcggtg atccggaaag
     3001 acgtgccgga cggctcgctc gcctactccg acgcgaagca gcggaacatc gagggctaca
     3061 accccaaacc cgccgaggac cgacccgagt gaccctcgag acccaggccc agtcgacccc
     3121 gcgcacaagc gtcccgcacg gctacgacaa gcggatgatg gtcgtgtccg ggcgcgccag
     3181 ccgcgagctc ggcctgaaga tcgccgacaa ggtcggggtg gacccggtcg aggccgacct
     3241 ccgcaccttc tccgacggcg aggtctactg ccgctacgag gagtcgatcc gcggcgccga
     3301 catcttcatc gtccagtcga tcgccggcaa cgaggtggag ggcatgaccg cgaacgacgc
     3361 gctcatggag ctcctcgtga tgatcgacgc ggcggtcggc gcctcggcgc accgcgtgat
     3421 cgccgtcgct ccgtggtacg gctactcgcg ccaggacaag aagtcggccc cgcgcgagcc
     3481 catcaccgcc cgcctcgtgg ccaagtgcct cgaggccgcg ggcatcgacc gcctgctcac
     3541 catggacctc cacgccgggc agctccaggg cttcttccag aagaccgtcg accacatgac
     3601 ggcgatgccg atcctcaccc agttcgtcaa ggaccagctc ggcgacgcca ccgacctcgt
     3661 gatcatctcg cccgacgccg gccgtgtgaa gctcacccgc aagttcgctc agaaggtggg
     3721 cgccccgtac gcgctgctcg agaaggagcg ccccgcccag caggtcgccg agatcggcta
     3781 cgtcatcggt gaggtcaagg acaaggtcgc cgtgatcgtc gacgacatca tcgacaccgg
     3841 cggcaccctc tccgccgcgg cgcagacggt cctcgacgag ggtgcctccg ccgtgtacgc
     3901 cgtcgccacg cacgggatct tctccggcaa ggcctacgag actctcggca actcgcctct
     3961 gtcgggcatc gtcgtcaccg acacggtccc gctgcgcagc ggcgcgcccg acaacatcaa
     4021 ggtcctcacc tgcaccgaca tcctcgccga ctcggtccgc cgcatcttca ccgacgagtc
     4081 cgtctcggag atcttcgccg gggagaacca gctcttctga gcctctacga gctcctcctg
     4141 accctccaca tcctcgccgc catcatctgg ctcggcggga gctggatgct gctgatcctc
     4201 gggctcacgc tgaggggcgc ggacctccag cggcgcatcg acttcacgcg catgaccgag
     4261 aaggttccga gcatcgtctt cgcggtctcc tcgctcgtcg tgatcctcgc cggctcgtgg
     4321 ctcgtccacg aggccggcta cgactactcc gacacctggg tcacgctcgg ctacgtgggc
     4381 tggttcatca gcttcctgtt cggggtcggc ttctacggcc ccgagggcaa gcgccgcgac
     4441 cggacgatcg aggccgaggg cgccgcggcg cccggcgtcg tgaagagcct caaccgcgtg
     4501 ctcaccgtcg cggcgatcga caccgcgatc atcacgctcg tcgtcgtcga catgacgacg
     4561 aagcccggtc tctagcccga gcgcgccgag aacttccgca cgcggcgctc gccgcgcgat
     4621 tcgaagaagg cccactcctc gcgcccgagc tcggtcgcgg gctcgtcggc gaaccaccac
     4681 tcgcagccga tccggtacca gttgcgcgag gcctcgagct ggccgagcac ggccatcagc
     4741 ccggtctcca tccgccgccc catcagctcg ttggcgggca cgttctcgcg gcgcatcagt
     4801 tggtagaact cggagcgcgg gtccgatatc gccgtgatca ccttcatcac gtagcgcggg
     4861 ctgatccgca cgtcgcggtc ctgcatgtac cagccgccga cggtcttcac gtgctccatc
     4921 agcttctcgg cgtcgatctt cttcgggttg ttgaggaagc cgaggtcgtg cagcgcgacc
     4981 cgcaggcgct cgggatcctc gtcgaacacc gcctcgagcg cggcgatctc cagctcgatc
     5041 tgctccatgt cgagctgctt ggtcatcccg aagtcgagga acgccacgcg gccgtcggcc
     5101 atcagcacgt agttgccggg gtgcgcgtcg gcgttgaagt gctggaggtg gtagatcgag
     5161 ccgaagcaga agcggaagac gatctcgccg aagcggtcct tctcctcctg cgggagcgcc
     5221 ttgatctcgt cgaacccgac gccgtcgacc cactcggtca cgagcacgcg ccgccgcgag
     5281 agccgcgtga tcacgtcggg cacgtagatg aacgggtgcc cccggtaccc gcgcgagaac
     5341 gagcgctggt tctgggcctc gtactcgtag tcgagctcct cgagcacgcg ctccttcagc
     5401 tcggccgccg ccgccttggc gtcgagcccg ggcgccagcg ccttcgcgag ccgcatgatc
     5461 atcccggcgt tctgcatgtc ggcgcggacg gcgtcggcca ccccggggta ctggatcttc
     5521 acggcgacct tgcgcccgtc gcagagcgtg gcgcggtgga cctgtccgat cgaggcggca
     5581 gcggccgcgg tctggtcgaa ctcgtcgaag atgtcctcga gcggctcgcc ctcccactcc
     5641 tcgtcgagca ccttcttcac cttgtcccac ggcatcggcg gcgcggaggt gcgcagctcg
     5701 ctgagcttct cctggtacat ctccgcgtac tcgggcggca ggaactcggt gtcgatgaac
     5761 gacgcgagct ggccgatctt catggcggcg cccttcatgt tcccgagcgc gtcgaccatc
     5821 cgctcggccg cctcgaggtg gcgcgcgtcg agcctctcgt ccgcggattc cttcgagcgg
     5881 accaggttcg ccgccttcgt gccggcgtac cgggcgccct ggccgccgat caccgttccc
     5941 accttcgccg tcctctggac gcggccggtc gggatctggg tctcgtcgtc gcgggccacc
     6001 gtgcgcaggc tacactcgtc cgccgtcatg gcagcccagc cccagcgtcc cagcctcggt
     6061 gtcgaggagc gctccgacaa cggttcccgc gcctcccgcc gcctccgccg tgaggggctg
     6121 atccccggca tcgtctacgg cggcaaggac tgctcgtcgg tgaagatcaa cgcgcgcgag
     6181 ctgcgcagcg ccctgatctc gagctccgcc gtgatcgacc tcaaggtcgg cgactccaag
     6241 gtcgtcccgg tgatcgtcaa ggacacgcag ctccaccccg tgcgcgacga gatcatgcac
     6301 atcgatctgc tcgaggtgga cctcaccgag aagatccaga cggtcgtgcc gctcgagctc
     6361 gagggcgccg aggaggcgcc cggggcgaag gagggcggcg tgatcgagca cgtcacgcgc
     6421 gagctgaaca tcgagtcgct gccgacgtcg atcccggagc gcatcgtcgc cgacgtctcg
     6481 cacatggagg ccgccgcgac gatgcacctc tccgagctga ccgcgcccga gggcgtcgtg
     6541 ttcctcgacg atccggagga gacgatcgtc gccacggtca cgatcccgac cgaggtggtc
     6601 gagcccgacg tcgaggagga gaccgagctg gtcggcgacg cggccgcgga cgtcgtgggc
     6661 gacgcggacg gcgaggccgg cggcgacgac tccgagggcg gcggcgaggg ctcctgagcc
     6721 tcttccggcg cggcgacggc cgcgtcgact ggttgatcgt cgggctcggg aaccccgggt
     6781 cgaagtacga ggggacgcgc cacaacgtcg gcttcgaggt cgcgaacctg ctcgcgcagc
     6841 gctggggcct ctcgcgcgcg aagaagaagt acgtggggct ctacacggac ggtcgcaccg
     6901 ggcccggcgg tccgcgcgtg ggcgtgctgc tgccgcagac ctacatgaac gaggcgggca
     6961 attccgtcgg tccggcgcgc ggggcgctgg gagcggagct cgaccggatc gtcgtgctcc
     7021 atgacgagat cgacctgccg ttcggcagga tcgaggaacg gctcggggga gggcttgcgg
     7081 gccacaacgg gctgaagtcc ctgaaggccg gcctgggctc gccggacttc cgccgcgtgc
     7141 gggtcggcgt ggggcggccg gacagcaccg acccggaggt ggtgagctcc tgggtcctgg
     7201 gccgcttcag cgagccgcgc gacgacgtgc gcgacctgat cgagcgcgcc gccgacgcca
     7261 ccgagcggtt gatcagcgcg gcctagactc gggtgcgagt ccttttgtgt gtcctatagg
     7321 acccagaaat ggactctcac ccggccgcat cgcggtcgct ccgcatgccc agttcgctac
     7381 gccaactgct gccgttcgcc tcggatgacc cggccttcgg gaggctcacc agcgcgctga
     7441 cgccgggcgc cgcggagcgc gcgttcgtat cgcagagcct gcgcccctac ctgattgccg
     7501 ccgtactcga cgacgagccg gagcggcccg gcctcatagt cgtcggtgac gaccgacagg
     7561 cgcgcgacct cgccgccgac ctcaagacct tcctcgcccc gcgcacggtg cgcctctacc
     7621 ccgcccgcgg cgtgcagtac gagtcgcacc tcgcgccgcc gccgcacctg gtcgggctgc
     7681 gcgtcgccgc gctcgacgcc ctcctcgcgg agccggacga gggcggtgcg gccgtggtgg
     7741 tcgccagcgc cgctgcgctc tgcgagaagg ttcccgaccc cgagctgcgc ccgcacggct
     7801 tcaccctcga gcgcggcggc ctggtcgacc tcgacgaggc ctccgcccag ctcgccgcct
     7861 gcggctacga gcgcgtcgac caggtcgagg agcgcggtca gttcgccata cggggcgaca
     7921 tcctcgacgt ctaccccgcc accgaggagc gcgccgtccg ctgcgagctc ttcgacgtcg
     7981 agatcgagcg cctcacgttc ttctccacgt tcacgcagcg cagcctcgag gaggccgagc
     8041 gcatcgagat cgcccccgcc gccgagatcg gccccgagca ccgcgagctc gcggagatgg
     8101 tcgcctcgga cgaggagcgt cccgacatcg cggagatcct ccccgtcgat cgcttcacca
     8161 ccttcctctc cttactgccg cagtcagcgc tggtggcagt cgcggcagag gaggagatcg
     8221 agccggcgct caccgatctt tggcacgacg taacgctgag cttccacgac aaggacgccc
     8281 accacctcta cgtggagccg cgcgaggaat tgcgcgagcg caccgccctg agcatcagcg
     8341 ccctcgccgg cgaccagccg caccagttcc gcgcccaggg cgccgacacc tccgcgaaga
     8401 acctgcgcga ggccgagccc gagctcgaga agctcgtccg ctccggctac cgcgccgtgg
     8461 tcgcctgggc ccgcaagggc gaggccgagc gcgccgcgta caacctcgcg cggacccgcg
     8521 cgaacttcct cgacggccag ccgcccgagc ccgggctgac gttcgcggtg gccagcctcc
     8581 gcgagggctt cctctctccc cagctgaagc tggccgttct gcccgagcac cgcctgctcc
     8641 ggcgcaagcg cggcgagcgc cgctcgggac ccggcgccgg ctcgaccgcc atcgcgagct
     8701 tcaccgacct gcgcaccggc gacgcggtgg tgcacgagga ccacggcatc gctcgcttct
     8761 ccggcttcga gacgaagacc gtcggcggcg tcacgcgcga ctacctggag ctcgagtacc
     8821 gcggcggcga ccgcgtcttc gtgccgagcg accagctctt caagatcagc cgctacgtcg
     8881 gggccgaggc gtcggacccg ccgctgtcga agctcggcgg caagcagtgg gagcagatga
     8941 agatgcgcgc ccgccgcgca gcgcaggcgc tcgcgggcga gctgatcaac ctctacgccg
     9001 agcgcaagcg ccgcgcgggc tatggcttcc cgcaggacga cgaatggcag ctccagttcg
     9061 aggcggcgtt cccgtaccag gagacgccgg accagctcga ggccatcgag caggtcaagg
     9121 ccgacatgga ggaggcgcgg ccgatggacc gcctgatctg cggcgacgtc ggctacggca
     9181 agaccgaggt ggcgctgcgc gcggccttca agtccgccgg cgacggcaag caggtgatgt
     9241 tcctggtccc cacgaccgtg ctcgcgcagc agcacttcgg cacgttcacg gagcgcctgc
     9301 gcgactaccc gttcacgatc gaggtggcct cgcgcttccg gtcgggcaag gagacgggcg
     9361 acgcggtgaa gggcttccag gaggggaagg tcgacatcct gatcggcacc caccgcctcc
     9421 tttcgcgcga cgtgcgcccg aaggacctcg gcctgctgat cgttgacgag gagcagcgct
     9481 tcggcgtgaa gcagaaggag ctgctgcgcc agctccgcct gcgcgtggac gtgctctcgc
     9541 tctcggccac cccgatcccg cgcacgcttc agatgtcgct cgccgggctg cgcgacatca
     9601 gtgtgatcga gaccccgccc gagggccgcc gcgccgtgcg cacctacgtg ggcgagtacg
     9661 acgaggacct cgtgcgcgag ggcatcgacc gcgagatcgc ccgcggcggt caggtctact
     9721 tcctgcacaa ccgcgtcgag acgatcgacg agaccgccga gcgcgtgcgc gcgatgtgcc
     9781 cgaacgcccg cgtgcgggtg gcccacggtc agatggccga gggcgagctc gagaaggtga
     9841 tgctcgagtt cctgcgcggc ggggccgacg tgctcgtctg caccacgatc gtcgaggccg
     9901 gcctcgacgt gcccaccgcg aacacgctga tcgtcgagcg cgccgaccag ctcggcctcg
     9961 cccagctcta ccagatccgc ggccgcgtcg gccgctcgcg cgagcgcgcc tacgcgtacc
    10021 tgctctaccc gagctcagcc gtgctcaccg agcaggccgc ggcccgcctc gccacgctct
    10081 cggactacac cgagctgggc tccgggttca agatcgcgat gcgcgacctc gagatccgcg
    10141 gcgccggcaa cctgctgggc gacgagcagt ccggccatgt ggcggccgtc ggcttcgagc
    10201 tctacatggg gatgctcgac caggcggtcg gccagctcgg tggcgacgcg tccgaggagg
    10261 cccccgagcc ggtccgcgtg gacgtgcccg tggacgccta cgtgcccggt gactacgtcc
    10321 cctacgaggc ggcgaagatc gaggtgcacc gccgcgtggc gggcgcgcgc gagatcgcgg
    10381 agctgatcct gctgcgcgac gagctcgagg accgcttcgg gcccgtgccc gagccgctcg
    10441 acaacctcat caagctccag gacgcgcgga tcaagctcgg gcgcgccggc gcgaaggatg
    10501 tcggcttcgc cggcggcaag ctgcgcgtct cgccggtgga gctcgcctca accgcggtga
    10561 aggagctgcg cgaggcgctc cccggggccg tgtacgagtc cggtcgcagc accgtggcgg
    10621 tgcgcatgcc cgacgaggcg gcggagcgct tcgcggcgct cgtgacggcg gcggagcaca
    10681 tcctcagggt cgccaccgag ccgccgacgg actagaggcg ttccgctagg atcgtgcgct
    10741 cgcgccgcag ggggatgctc cgcgctggcg cttagacgcg cttgaacctc agcgacttga
    10801 ctgcccgaat gctcagaacc cttcgtctca ccgccctgct cgcggccatc gcggccgtag
    10861 ccctcgtcgc caccggctgc ggcaacgacg tgcccgccgg cgccgtcgcc aaggtcggcg
    10921 acaaggagat caagaagtcg gagttcaacc gctggctcca ggccgccgcg cgccagcagg
    10981 cgcagagcca gccgggccag gcggccgccc cggtctcggt tcccgacccg cccaacttca
    11041 cgaagtgcat cgacgcgaag cgcaagtcca cgccgcccgc gcagggcgcc gctccaccga
    11101 acaacgagca gctcaaggcc cagtgcaagc aggagttcga cgggctcaag gagcagacga
    11161 tgcagttcct gatctccgcc gagtggctcc agcaggaggc caagaagcgc ggcgtcgacg
    11221 cctcgccggc cgaggtgcag aagaccttcc aggagcagaa gaagcaggcg ttcccgaagg
    11281 aggccgagta ccagaagttc ctcaaggagt ccggccggac cgagcaggat ctgctcttcc
    11341 aggtcaagct ctcggtgctc acgaaccagc tccaggcgaa ggtcgtcgag ggcaagggcg
    11401 acccgaagcc ggacgagatc gaggactact acaacgagaa caagaagcgc ttctccacgc
    11461 ccgagagccg cgatctgctc gtcgtcctca ccaaggacga ggccgacgcg aagaaggccc
    11521 tcgccgagct gaagaagggc aagccctgga aggacgtcgt caagaagtac tccgaggaca
    11581 ccgcctcgaa ggcgcagggc ggcaagcttc cgggcgtcac caagggcacg caggagaagg
    11641 cattcgacga cgcgatcttc aaggccccca agggccaggt ccagggcccc gtcaagaccc
    11701 agttcggctt ctacgtcttc caggtcgaga aggtcacgcc ggccacgcag cagacgctcc
    11761 agcaggcgac cgacacgatc aagaacctgc tcaagtcgca gtcccagcag aaggcgctga
    11821 gcgacttcgt cgaggacttc cagaagcgct accgcgagga gacggtctgc gccgaggact
    11881 acaagacgag ccagtgcgat aacgggccca agccgccgga gcagcagcaa cagcagcagc
    11941 agagccagcc gcagggcgcg cctccgacca aggactcggg ccagcccgcg cagccggtcg
    12001 tgccgggtgg cgttccgggc caaccgggcg ctccgggcca ggtcccgcag ggccaggtgc
    12061 cccagggcgg tgctccgccg cagggtgcgc agggcgcccc gccgcagcag gcgccgccgc
    12121 agcaggctcc accgcagcag gccccgccgg gcggctagcc cgagccgggt ggccgaagcc
    12181 gatagcgccg ccgacccagg ccgggcggcc gaagccgacc gcgccgccga ggcggtacgc
    12241 cagctcgacg agctgacccg ccgcctgcgg gtcgagtgcc cctgggaccg cgagcaggac
    12301 gagcgctcga tcgtcccgca cacgatcgag gaggcctatg agctggcgga cgccgcccag
    12361 cgcggcgacg actccaagct gcttgacgag ctgggcgacg tgttgttcca ggtgcacttc
    12421 ctgtcgctcc tgctcgagga gcgcggcgcc ggcggcctcg ccgacgtggc cgaccactgc
    12481 cgcaccaagc tgatccgccg ccacccgcac gtgttcgggg aggtggaggt cgagggcgcc
    12541 gccgaggtcc tgcgcaactg ggaccagatc aagcagggcg aggcgggccg ggaggccggc
    12601 atcttcggcg aggtgccgga gatgctgccc gggcccctct acgcgcgcaa gctgctgcgc
    12661 cgcgcgggcg gcgccggtca cgagtggccg cccgagggcg cgcgcgagcg cctgcgcgcc
    12721 gcggccgagc ggatcgaggg cgtcgacgcg acgcccggca ccgagccggg ggtgatcctg
    12781 gacggcgggc gcgacgagcg cttcgacctg gtgggcgacc tgctgctggc cgccgtcgcg
    12841 ctcgcgcgcg agctgagggt ggacccggag ctggcgctcc gcgcggccgc cgaggacatg
    12901 agagccgaga tcgaaggggg agggcgatga gtcagatcga gcagatccac gcgcggcagg
    12961 tgctggacag ccgcggcaac ccgacggtcg aggtggaggt cgttctcgac tccggcgcct
    13021 ccgggcgggc gatcgttccc tcgggcgcat cgacgggcga gttcgaggcg gtcgagctgc
    13081 gcgacggcgg cgaggactgg ggcggcaagg gagtcgcgac cgcggtcggc aacgtcaacg
    13141 gcgagctggc cgagtcgctg cgcggctccg acgccgcgga ccagcagggc ctcgaccagc
    13201 ggatgatcga tctcgacggc acgcccaaca aggggcggct cggcgccaat gcgatcctcg
    13261 gggtctcgct ggccgccgcg aaggcggccg cgaacgagaa cggcctcacg ctctggcgct
    13321 acctgggcgg cgaggccgcc cgcatcgtcc cggtcccgat gatgaacgtg ctgaacggcg
    13381 gcgcgcacgc cgacaaccgc gtcgacttcc aggagttcat ggtcgtcccg gtcggggcga
    13441 gctcgttctc cgcgggcctg aagacgggcg tcgaggtctt ccacgcgctc aagcgcacgc
    13501 ttcacgaccg cgggctgtcc acggccgtgg gcgacgaggg cggtttcgcg cccgacctcg
    13561 actcgaacga ggcggcgctc gggatgttga tcgacggcat cgaggccgcc ggctaccggc
    13621 ccggcgacga cgtggcgatc gcgctcgacc ccgctaccag cgagctgcac gacgccgggg
    13681 tctacgacct cgagcacgag ggccgcaagc tctcctccga gcagatggcc gactactggg
    13741 ccggcatcgc ctcgcgctac cccgtgctgt cgatcgagga cggcatggac gaggaggact
    13801 gggacggctg gaaggcgctc accgaccgca tcgggcagac cgtgcagctc gtcggcgacg
    13861 acctgttcgt gaccaacacc gagcggctgc gccgcggcat cgacagcggc accggcaact
    13921 cgatcctgat caaggtcaac cagatcggca cgctcacgga gacgctcgac gccgtgcgca
    13981 ccgcgcagga ggcgggctac acagcggtga tgtcgcaccg ctcgggcgag accgaggacg
    14041 tgacgatcgc ggacctcgcc gtcgcgaccg gctgtggcca gatcaagacg ggcgctccgt
    14101 cgcgctcgga ccggaccgcg aagtacaacc agctcctgcg catcgaggag gcgctggggt
    14161 ccgacgccga gtacctgggg cggtcggcct ttcgcagcta ggaggagggc cgggacgagc
    14221 ggcgaagctc gccgcgatgg cacgcgccgc agccgccccg gcaccctcac gccgcagccg
    14281 cacgaccacc gcgccgcgcc gcgtgggcgc cggcatccac tgggagcggg tgggccggct
    14341 ggcgctgctc ggggtgctcg ggatcatcgt cctgctctac atcccgccgg tggcgcactg
    14401 gttccagcag tcgagcaccg ccgaccgcag cgagcagcag ctccgggagc tagagaccga
    14461 gcgcgcgcgg ctcaggagcc gcctgcacga gctcagcggg cctggggcga tcgagcgcga
    14521 ggcgcgcaag ctgggcatga cgaagcgcga cgagcgcccg tacgtgatca ggccctagcg
    14581 cccgccgtct gccgcggccg agcgccccgc cgtgcgcggc gcggcccctc gctaccttcc
    14641 gcggcgccga gcgccccgcc gcccgccgct accttccgcg gccgagcgcc ccccgtgcgc
    14701 ggcgccgccc gccgctacct tccgcggcgt gtcctacgcg ctcgagaacg cgatcttcca
    14761 gtgggaggag ggcgagcgcc acctgcgcga cgccgagccc gccgagaggg tgcggctcca
    14821 gcacgccgcc gaccgcgtcc tcgaccggct gcgccagcga ctcggctcgg agttcacgat
    14881 ccaggagctg agcgacctct acgcgtccga gccggactgg acgacggact ccaccccgcc
    14941 agcggccatc gacgccgcgt tcgcgcgtta cgcgcgggag gccgccgact tcggcggcgg
    15001 gcgcccgcgg gcgcgcttct aggcgtcgaa ggagtctggg tcgagaccgc caccagcggc
    15061 ctcgtcgccc tcggcgccgg gctcgccggt gccgcccgcg gcggcatcct cgccgagcgg
    15121 cagatcgtcc tcgccgagcg gtgcggcgtc ctcgccgacc ggagcgtccc cgccaagcgc
    15181 cgcggcgtcg tcgtccgaag tcgaggcgtc cgcgtcggaa gtcgccgcgt cctcgcccga
    15241 gcctgcgtcc tcgccggagc ccgacccacc ggccgcgccg cccgcagcgt cccc
//
LOCUS       DASVPL010000321        21047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_117194, whole genome shotgun sequence.
ACCESSION   DASVPL010000321 DASVPL010000000
VERSION     DASVPL010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 21047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21047
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117194"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1904
                     /locus_tag="VF517_18395"
     CDS             180..1904
                     /locus_tag="VF517_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX8054959.1"
                     /translation="MTLVTRPNAWVAVEDDPATQLTYAPRVDPAPLTVSVPGRDATLG
                     SLVIVITNQTATDLAVASITFTITVGQPGVAGTPLMPTTAGSHALVSDTTTWSLAWPG
                     PAAAPAGTVDCALTPATGSTATLAAGASVYVQIYDFQTVQVPSTSTVLVTETIGTSDP
                     AFTNIGVSTFPDGFFFDSLTVNAGPSNALVPVAQVDRGTGVTLTWNSSLADTKDQTVY
                     WSSATAGQQHATPTKLGEWSTPPDAPLTSDTVFVVVVKASGAGDEPLTASLATAVSVR
                     NPALVASSATVSGDVHSATGTVDGLLHAGTAKVDGDLHAATGTVDGLLHAGSGKVDGD
                     LHAATGTVDGLLHAGSGKIDGDMHAATGTVDGLLHAGSASVGGNVQAGSLSAGTVTVT
                     SKLDVSKASVSILGSAITLAKLGTYSVNADSLVMVTTPFFQQYPSPNTSYYITGTCGP
                     TTMIAVSGDWSPLGAGIRVSGSFVLPVPAGQQFTLAVGQNGWVYASYAKLTLVFLGTP
                     PPGDEPAVELVAEAGPPDPEIEKAMTEAAAQQERRADEFVGLLEEAIGKPVGDELKQK
                     LVATLRGA"
     gene            1923..3386
                     /locus_tag="VF517_18400"
     CDS             1923..3386
                     /locus_tag="VF517_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX8054960.1"
                     /translation="MIARPNAWVAVDDGSATQLTYAPRVAPAPLTVSVPGRDPTLGSL
                     VVVITNQTVADVAVASVTFTIVVGESGVGGAALMPATAQERELVSDPGTWSFGGPGQQ
                     VTAGTADYTLAPATGDAAVLAAGESVVVTLSGFETLDTPGTSTIVVAESVEGTDPAYT
                     SIAVSTFPEGFFFDRLTVSVPSAGNLVPVAQVPSGSGVTLTWNSSLVDTEKQTVYWSS
                     PTGGQQQATPQQLGEWSTPADAPLTSDTVFVVVVEAAGGDSGPMTASLSTAVAVQDPE
                     LVASSASVSGALSAGSATVGGSIAARGDISAGGRVEAASAAVAGTLEAGSAHVTGDVQ
                     ADTLTAAGATITSSLSAQEGTVSVLTNAETISVPRNRGFGGSAAFLAHTDGLVVAAVT
                     WPPLEDLGAGPVSVVRASGTSGILALRAGGEQRDPNVYEPFGDSFVLPVAAGAQCVLE
                     WAQLPTEPSLVSFSWVALGSPPPGKQSLEEQQQGGQP"
     gene            3383..4810
                     /locus_tag="VF517_18405"
     CDS             3383..4810
                     /locus_tag="VF517_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054961.1"
                     /translation="MMIARPNAWVSVDEEPATVLTYAPRVDPAPLTVSVPGRDPTLGA
                     LVVVITNGTPADVQVSSVTFTITVGQTGPDGPPLTPTGEGVKSLVSDTTNWSFDGPTS
                     QVTSGSADYVLGPQTGSTVTLAAGASVYVEIYDFQTAESPSTSTVTVEEVIADGDPTF
                     TNIGVSTFPDGFFFDSLAVTVPSDNALTPVAQVAGGTGVTLTWNTSLVDARNQTVYWS
                     SPTSGQQQATPTKLGEWSTPPDQQLTSDTVFVVVVRAQGVRNEWMTASLATAVSVLDP
                     ALVASSASVSGALSAGSVQAASATVSGGLEAGTLTAGTGTFGVLLAAGSATVNMLGGP
                     DTIPLQPSGPYPSFTNYVANTDGMVIGAVTWPDIDPSVGTAWAMACGTSGPLQLYTGG
                     TRSDDSGPKLAGDSFVLPVAAGAPFSLWSMLQGRAGSGSARFTWMSLGSPPAGQPPYE
                     QIGTAEPVDLAALGLPNLPPQEGTD"
     gene            4813..6585
                     /locus_tag="VF517_18410"
     CDS             4813..6585
                     /locus_tag="VF517_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054962.1"
                     /translation="MMITRPNAWVSVEDDPTTQLTYAPRVDPAPLTVSVPGRDPTLGS
                     LVVVITNGTPADVEVSSVTFTIVVGTTGPEGPPLTPTTEGVKSLVSDTTNWSFNGPPS
                     PRTSGTADYVLGPATGSTVKLAAGASVYVEIFDFQTVQVPSTSTITVAEAIGGGDPQF
                     TNIGVSTFPDGFFFDGLTVNVGSGSALAPAAQVVNGTGVTLTWNSSVVDTKNQAVLWS
                     SATGGQQHAIPTKLGEWPTPAGLTSDTVFVVLVTAQDVGGEPMTASLATAVSVQNPAL
                     VASSIQTGQSTVSGNETVGGTLTATGGIAANGGLTATAATVNGALNANGGLTTNAAMV
                     NGALMASGGIAANGGITASAAAVNGALTASGVVNANGGLNATGLSVSGEARVTGFTTL
                     SYLNLTRLFMNTDVMNSIYAINNSAQYPTGYFQNNVGRSGNQVTGVYAKVAQGSDWGL
                     YTNGRSGSDNSAVTHVETRDGHRVVTSPLVLEAEVQVSGQARLRKGRATVELGADGAD
                     MILHGEEHSYRVLVTPTARCNGLAVTSKDDACFEVEELLDGDSDAEFDWLVIGRKRAA
                     LGSSHADVLPESLPVVEEPPPMDH"
     gene            complement(6634..7410)
                     /locus_tag="VF517_18415"
     CDS             complement(6634..7410)
                     /locus_tag="VF517_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054963.1"
                     /translation="MCIDGVAAVDLAVWRLIPYQAQWPEFYSYARAAFDLTDDSWNPA
                     GGGAPDDARLPYGKMINSIYLLTYAIRNEYLPQFHSREDYMLAANGADNAFHDDYEYQ
                     FRNRNDGLSAKAIESGSRTEMYCPVFNVGVYVDDPARRASVMVHEGWHHWQYKYNWQS
                     HHEFGGAITGGQEGDKFYFHGTGAFDFGQLHTIDLGANPIKFHSPYQVEAEFLADLAE
                     YSFGWIPIAVTDSAKAFGNDILANRFQNATSYRIGQPRPF"
     gene            complement(7400..8167)
                     /locus_tag="VF517_18420"
     CDS             complement(7400..8167)
                     /locus_tag="VF517_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054964.1"
                     /translation="MKGPEEMDLGRGKTSAYAYPPARQEIEPKTESPTLPLPPPRDRE
                     ALSALIEAVASTPNGRRDDVRRMIGEWGERSAVADVLHQELHSLPTSDVGRHGLLLSI
                     IGELAEPSSVDALERFLWMSKQEIYGPSPEPADGDGCGFEPVGILEARAAEMLAWVLK
                     TDQEDRVMKIVLDHPLGAVRAAAIDAYLFNRDDDPDAAAELAKRVRSADAWAVGLPRR
                     FDGMYAEDFERLAAEHAAQHGHPPEMPHRNERGHGVH"
     gene            8280..9257
                     /locus_tag="VF517_18425"
     CDS             8280..9257
                     /locus_tag="VF517_18425"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054965.1"
                     /translation="MRLFTALRRPALALFGAALVACLALALASTAQARTDDRAKDVYW
                     IHGFSHNESIDCAKEFAVMKERFRSWGFTGRWATVGYYTGDKNCTMAEGHGSYHSRHA
                     GGHSRDRHTVNDPLEHIAYHLAWLIYDLSTKHGRTVDVVAHSMGGLLIRYAIAQTRNH
                     DPDFPPSLRIEDVVTMGTPHGGVRNSWLTIGCGTFAYQCREMSAGSDFLKWLEDDDRN
                     SNGWNPQSSVPTDWSTFGSDDDDFAAADRAAGTARDRSPSHDYIGSCHKTWYKTADDI
                     EHGDFKTDASSALTADVYRYNCNGGGWRSDPDSHWPIRRAARALTLGTE"
     gene            9281..9796
                     /locus_tag="VF517_18430"
     CDS             9281..9796
                     /locus_tag="VF517_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054966.1"
                     /translation="MGTMFDTVGDPKETFSGLEVEAVAAYGNGSYANYTRAKEEFPHV
                     PVLEIDVSGEGIGDAGDFERGDMSYADAGPWAKRRIAAGVHRPVVYFSVSNWQAVKQS
                     LSEAGIHPSDVRLWTAHYTGEAHLCSSACGFGDKPTDHADATQWTDPDTAPAPYRGRN
                     IDVSETAANFF"
     gene            complement(9818..10591)
                     /locus_tag="VF517_18435"
     CDS             complement(9818..10591)
                     /locus_tag="VF517_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054967.1"
                     /translation="MVAAACIATALWSAAPAAAQVPSLTGEQLVATGEVFAFPAAATP
                     GEFTTTGTCTPDGTSTFSFTATGPAFGPYPGTFEEQGTFSLVPDPNAPNGFSLSAFHA
                     TFTIDSVNGEVTGTKEFAELVPGAGPSCREADQYQVSSGASLTNYEAVIRPATGGTYT
                     TEGQAESSSLYNSDAPGEPANFADFSEMFLTGTPPVSQGDPDPDPDPEPPRPTTPEEC
                     KAGGFRDFAELGFSSQGDCVAFVQTGGKNEPGRDVPRSR"
     gene            10712..13549
                     /gene="leuS"
                     /locus_tag="VF517_18440"
     CDS             10712..13549
                     /gene="leuS"
                     /locus_tag="VF517_18440"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX8054968.1"
                     /translation="MSAPDSQTTSTPAGHRYDAALANAIEAKWRERWATDGVFLSPNP
                     RGELAEPNHGLTERPKLYVLDMFPYPSGEGLHVGHPLGYIATDIYARFKRMAGFNVLH
                     TMGYDAFGLPAERYAVDTGQHPEVTTRNNVANMGRQLHRIGLGHDERRGVSTTDVEFY
                     RWTQWVFLQIYDSWYDEEADRARPVEELVAELDAGRRGLPDGRDWRSLDERERRRAID
                     AQRLAYLDEVTVNWCPALGTVLANEEVTVDGRSEEGNFPVYRRPLRQWMLRITAYADR
                     LVEDLAELEWSDSLKAMQTNWVGRSPGAVIRFGLEAPGADELEVFTTRPDTLFGATFV
                     AIAPEHPLLEGDLPAETAEACAAYREEATRRSDRQRQVETEPSGVYTGLDAIHPLTGE
                     RVPIYAADYVLIQYGSGAVMGVPAHDSRDHAFARAFGLPIPVVVQPPPDWLAEHGLSE
                     DTPAAEWPDAYTGAGTSVNSGPAFDGRPTEEAHRLIVEALVEAGRGERRVGYKLRDWL
                     FSRQRYWGEPFPIVYDEHGDPHAIPDSMLPVELPELEDFAPVVDEDSTAEPEPPLARA
                     ADWVEVELDLGDGPKRYRRETNTMPQWAGSCWYYIRYVDPRNHEAFADPDNERYWLSS
                     EGQIGGVDLYVGGVEHAVLHLLYARFWHKVLYDLGHVSSPEPFQRLFNQGMVQAAAYT
                     DERGFYVNAAEVEERDGRHFHGGGEVTRHYGKMGKSLKNGVSPDEIIDSYGADALRLY
                     EMFLGPLDQNRPWETQGITGVARFLRRVWRMVVDPETGDVVVGEGAPPDATLRALHRT
                     IATVRSGIDRLHFNTGIAALMELSNDLRDTYAEGAVPRQVAEWFVLALAPFAPHMCEE
                     LWERLGHTTSLVYATYPDPDPALLEREVVTIAVQVNGKLRGSFDAAPNTDRESLEETA
                     RGLPNVASHLSGRTVKRAVVVPGRLVNFVTD"
     gene            13627..13950
                     /locus_tag="VF517_18445"
     CDS             13627..13950
                     /locus_tag="VF517_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054969.1"
                     /translation="MDTTQGAVLELVVFKLKDGAAREQLLGTVDAVSAWVAEQPGFVG
                     RELAHDRGGDRWIDIVRWRSLEDAHAAAELAMSSESCAPMFGLINMESMLMLHAEPAI
                     APVHA"
     gene            13947..14414
                     /locus_tag="VF517_18450"
     CDS             13947..14414
                     /locus_tag="VF517_18450"
                     /inference="COORDINATES: protein motif:HMM:NF039682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HEX8054970.1"
                     /translation="MTARRALEAEEILSELLGLDPGLTRERYWGEDAVFYNPGRAAPL
                     GVILAAIKDHDGPNDRSAQLDRPGVYRFAFGMAPDSYERRFGAHPGRPPKGGVIDLPH
                     YDPARVGRLMPHPVYGWMSWVQILAPTARTFAPLRPLLLESLEIARAKWSRRA"
     gene            14425..15141
                     /locus_tag="VF517_18455"
     CDS             14425..15141
                     /locus_tag="VF517_18455"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8054971.1"
                     /translation="MPLPPDIDSLVARAQRGDAAALEAVCARARPLLVRHARRLLGRS
                     ADAEDAVQEVLILTCARIGTYRWEGSFAGWLYAIATRSILRRAARTPRDAALAAELAD
                     RALVSDYEPMTEAEWRLVEQDVHMACTVGVLTELSPETRRLYLLGEVLAVPDRVGAEV
                     ARTTPAAYRKRLERARRAVAAAVRAHAQPSEDDGRVAAAARELDDLLRLGELHRAHAR
                     PGTPAGALRALERVAPTLAS"
     gene            complement(15153..16124)
                     /locus_tag="VF517_18460"
     CDS             complement(15153..16124)
                     /locus_tag="VF517_18460"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054972.1"
                     /translation="MSRAQSPQVIVASDEGSPTVASLVECVASLGGQAHVLSTRRLPR
                     EGCVALDACGAEIRLGGDPIRLDACRGIWNWHAEPPLASDDDASTARYVRHEWELTLR
                     GLAATAPESIWINHPLRASWLDGNKLAQMKLAARAGFDVPPTLLSNDPDRIVRFAQQF
                     DQVAVKSQGGAWRETPDGAMAVAYTQRCTSAELDSSRTGLTRAPVLVQPYLEKACELR
                     VTVVDRTVFVCRIDSQASARTEVDWRRDVNAVSHELVEAAPDEVDRIRALVDAAGLRY
                     AAIDLACTHDGKTYFLDLNPAGQFGWIETRTGAPILRTLAEALLRPA"
     gene            complement(16121..16315)
                     /locus_tag="VF517_18465"
     CDS             complement(16121..16315)
                     /locus_tag="VF517_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054973.1"
                     /translation="MAERSSDEIATGIESATAAEPFLMSKAEFVSHLEAQGGTGCGGY
                     STVEHADGKRIVAWDYACKD"
     gene            complement(16393..17451)
                     /gene="ligD"
                     /locus_tag="VF517_18470"
     CDS             complement(16393..17451)
                     /gene="ligD"
                     /locus_tag="VF517_18470"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8054974.1"
                     /translation="MPDATTVEAGGRDLRVTSPDRVLFPATERSPALTKLDVVNYYVA
                     VGDGIMRALARRPTTLERWPKGVHPGMVLSTREKGGGDAFFQKRIPKGAPDYVETARI
                     QFPSGRHADEICPTEVAVVGWAAQMGTITFHPWPVRSDDVDHPDELRLDLDPQPGTDF
                     DDAVRVAATARALLDDLGYVGFPKTSGGRGVHIYVRIEPRWTFTDVRHAAIAFGRELE
                     RRLPGEVTTKWWKEERGERIFVDYNQNARDRTIASAYSIRPKPGAPVSAPVTWDELPE
                     VAPEDFTVATMPARFAAVGDLHAAIDDAAHSLEPLLEMYERDEAEGEADMPYPPDYPK
                     MPGEPKRVQPSRDRDRPR"
     gene            17626..18036
                     /locus_tag="VF517_18475"
     CDS             17626..18036
                     /locus_tag="VF517_18475"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8054975.1"
                     /translation="MPIGFILRAATRDAQAAAQAALDPAGITLREYGALTVLREDGPL
                     SQQELAQRLGIDRTSTVFLIDRLEELKLVKRRRSPADRRAYALELTRTGTRVQGRAQA
                     AMAEQESRFLSGLTQRERQQLVDLLMRLVEAPSD"
     gene            complement(18058..18342)
                     /locus_tag="VF517_18480"
     CDS             complement(18058..18342)
                     /locus_tag="VF517_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054976.1"
                     /translation="MTNRRMTQSGPERPADGPSGGDEGAAGEARQVTPGTTLWLLAMA
                     GERIGCEPAAEAHPSAEPPLGARPPLLRGLGRRLAEVAALEGLLVRWRRL"
     gene            complement(18362..19690)
                     /locus_tag="VF517_18485"
     CDS             complement(18362..19690)
                     /locus_tag="VF517_18485"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX8054977.1"
                     /translation="MTELAPTAPPPSGRRSEPASDGWVIDAIRRHLSPGLALGYKMAG
                     RDAVEVEADGATVTLSDGRRAVDFGSYGVTLLGHRHPAVVRAVERQLGSMPTATRTLA
                     NPAVAAFAVALLDRFDGAFPRLWIGTDGADAVEVAVKLARRRTGRLRVLALQGAFHGK
                     TLGALALTASPAFRTGLEPLLGHVTRVGRDDVAGVEAELERGDVAALVFEPVQGEGGV
                     RRLEPDVMRRWSAAAHDAGAYVISDEIQCGLRRCGPFSIARAEGAEPDAVLLGKALGG
                     GVLPLSAMVATDDLYSPLARDPTWHTATFGGHPLACAAGTAALDAIDAAAVRAREIAA
                     RCERELDDLAASRHAVVSEVRGRGLLWGIQLRSAGLAGTVLVDLAEQGLLVSPCLSAP
                     ETIRLLPPMVATDAELDFAVDALGHALDEAESVGDDDAARTESGSALRRA"
     gene            complement(19706..20167)
                     /locus_tag="VF517_18490"
     CDS             complement(19706..20167)
                     /locus_tag="VF517_18490"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8054978.1"
                     /translation="MPEVALDMEIRAPAAVVWDAIVAIDRYPASMENVRSIEILDDER
                     EGKRRSVWSVLLKGSILEWEEVETIDSQRLTMTFEQVAGDMERLDGEWVVTPEGDALT
                     SVRLRVSFDIGIPLLADMLNPVAQRALRDNCAEMLRGVEHTALERPAPAPR"
     gene            complement(20160..20678)
                     /locus_tag="VF517_18495"
     CDS             complement(20160..20678)
                     /locus_tag="VF517_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054979.1"
                     /translation="MRTIDVDARRYAWTHGHAMWRMGLPIVALLEDMRGGLAAEQDGL
                     VRHTARTVGQNCAVVLNMVLNHERPLPPPRMRVAWALERLSGHELGGECWELVRSSYE
                     LTPAELVARSERLVERVREVVGEIPDPLSPEGYYPALAFARDWLTFIDAMGEEGFLPK
                     EWTRSQQGEARA"
     gene            complement(20675..>21047)
                     /locus_tag="VF517_18500"
     CDS             complement(20675..>21047)
                     /locus_tag="VF517_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054980.1"
                     /translation="SDVGGFVAGATELAERMTGMELAPERLAPLFAWSPAGLRSFALG
                     GERVLVATEPGMVWRIEEAEAAAWGRLVDRYGEGGPWPAAECDPAEASLAVELYERGV
                     VAMVWSRGVTAAELTHVGEGS"
BASE COUNT         3090 a         7602 c         7451 g         2904 t
ORIGIN      
        1 gacttcgtgc cgctaccgaa gccgaggcgg gccgtgcgga cgggggcgga ggaggcggtc
       61 accctgctcg agccgttcct cgcgaagccg ctcgacgaca gcacgaagga gaagctcgcc
      121 gacgcactcc gcagcgcgta gcgctcgcgg ttcaccccta caccccggaa gggagcagca
      181 tgacgctggt cacccgcccc aacgcatggg tcgccgtaga ggacgatccc gccacccagc
      241 tcacttacgc cccgcgcgtc gatccggcgc cgctgacggt cagcgtgccc ggccgcgacg
      301 cgacgctcgg gtcgctcgtc atcgtgatca cgaaccagac cgcgacggac ctggccgtcg
      361 cgtcgatcac gttcacgatc acggtgggtc agccgggcgt cgcgggaacc ccgctcatgc
      421 cgaccacggc cggctcgcac gcgctggtgt ccgataccac cacgtggtcg ctggcctggc
      481 cggggccggc ggcagcgccc gcgggcacgg tcgactgcgc gctcacccct gcgaccggca
      541 gcaccgcgac gctcgccgcc ggtgcgtcgg tctacgtcca gatctacgac ttccagacgg
      601 tgcaggtgcc gagcacctcg accgtgctcg tgaccgagac gatcgggacc agcgacccgg
      661 ccttcacgaa catcggcgtg agcacgttcc ccgacggatt cttcttcgac agcctcacgg
      721 tgaacgcggg gccctcgaac gcgctcgtcc cggtcgcgca ggtggaccgc gggacggggg
      781 tcacgctgac ctggaactcg tcgctggccg acacgaagga ccagaccgtc tactggtcga
      841 gcgccaccgc cggtcagcag cacgccaccc cgacgaagct cggcgagtgg agcaccccgc
      901 cggacgcccc gctcaccagc gacaccgtgt tcgtggtggt cgtgaaggcg agcggggccg
      961 gggacgagcc gctcaccgcg tcgctggcga ccgccgtctc ggtgcggaac ccggcgctgg
     1021 tcgcctcgtc tgcgaccgtg agcggcgacg tccactccgc caccgggacg gtcgacggcc
     1081 tgctccacgc gggaaccgcg aaggtcgacg gcgacctcca cgctgcgacc gggaccgtgg
     1141 acgggctgct ccacgccggc agcggaaagg tcgacggcga cctgcacgcc gcgaccggga
     1201 ccgtggacgg cctgctccac gcgggcagcg ggaagatcga cggcgacatg cacgccgcga
     1261 ccgggaccgt ggacgggctg ctgcacgccg gcagcgccag cgtcggaggc aacgtccagg
     1321 ccgggtcgct ctccgccggc accgtcacgg tcacctccaa gctcgacgtg agcaaggcga
     1381 gcgtgtcgat cctcggtagt gccataacgc tcgccaagct cggcacctac tcggtcaacg
     1441 cggactcgct cgtgatggtg accaccccgt tctttcagca gtacccgtcg ccgaacacct
     1501 cgtactacat caccggcacc tgcggtccga cgacgatgat cgccgtcagc ggcgattggt
     1561 cgcctctggg agccgggatc agggtgtccg gctcgttcgt cctaccggtc cccgcgggcc
     1621 agcagttcac gctcgcggtg ggccagaacg gttgggtcta cgcgtcgtac gccaagctca
     1681 ccttggtatt tctggggacg cccccgcccg gagacgagcc ggcggtcgag ctcgtggccg
     1741 aggcgggacc acccgacccg gagatcgaga aggcgatgac cgaggccgcc gcccagcagg
     1801 aacggagggc ggacgagttc gtcgggttgc tcgaggaggc gatcggcaag cccgtcggcg
     1861 acgagctcaa gcagaagctc gtcgcgaccc tgcgcggcgc ctgacgagga gccacgcccg
     1921 cgatgatcgc ccgcccgaac gcctgggtcg ccgtcgacga cggctccgcc acccagctca
     1981 cctacgcgcc gcgggtggcc cccgctccgc tcaccgtgag cgttcccgga cgcgatccga
     2041 cgctcggctc gctggtcgtc gtgatcacca accagacggt cgcggacgtg gcagtcgcgt
     2101 cggtgacgtt cacgatcgtc gtcggcgagt cgggggtcgg cggtgcagcg ctcatgcccg
     2161 cgaccgcaca ggagcgcgag ctcgtttccg acccggggac gtggtcgttc ggcgggccag
     2221 gccagcaggt cacggccggc acggccgact acacgctcgc gcccgcgacc ggcgacgccg
     2281 ccgtcctggc ggccggcgag tccgtcgtcg tgacgctctc cggcttcgag accctcgaca
     2341 cgccgggcac ctcgacgatc gtggtcgccg agagcgtcga ggggaccgac ccggcctaca
     2401 cgagcatcgc cgtcagcacg ttcccggagg ggttcttctt cgaccgcctc accgtgagcg
     2461 tgccgagcgc gggcaacctc gtcccggtcg cgcaggtgcc gagcgggtcg ggcgtcacgc
     2521 tcacctggaa ctcgtcgctc gtcgataccg agaagcagac ggtctactgg tcgagcccga
     2581 caggcggcca gcagcaggcg acgccgcagc agctcggcga gtggagcacg cccgccgatg
     2641 cgccgctcac gagcgacacg gtgttcgtgg tggtggtcga ggcggccgga ggcgacagcg
     2701 gccccatgac ggcgtcgctg tccacggcgg tcgcggtcca ggacccggag ctcgtggcgt
     2761 cgtcggcctc ggtgagcggc gcgctcagcg ccgggagcgc gaccgtcggc ggctcgatcg
     2821 ccgcgcgcgg cgacatctct gccggcggcc gcgtcgaggc agcttcggcc gcggtagccg
     2881 ggacgctgga ggccggctcc gcgcacgtga ccggagacgt ccaggccgac accctgaccg
     2941 cggctggcgc gacgatcacc tcgtcgctca gcgcgcagga gggcacggtc tcggtcctga
     3001 ccaacgccga gaccatctcc gtcccgcgga acaggggctt cggaggctcg gcggccttcc
     3061 tcgcgcacac cgacgggctg gtggtcgcgg ccgtgacctg gcctcccctc gaggacctgg
     3121 gcgccggtcc ggtctccgtg gtgagggcat ccggcacgag cgggatcctc gcgctccgcg
     3181 ccggcggtga gcagcgtgac ccgaacgtct acgagccgtt cggcgactcg ttcgtgctgc
     3241 cggtggcggc cggggcgcag tgcgtgctcg agtgggctca gcttcccacc gagccgtcgc
     3301 tggtcagctt ctcgtgggtc gccctcggat cgccgccgcc cggcaagcaa tcgctcgaag
     3361 agcaacagca aggaggccaa ccatgatgat cgcccgcccg aacgcctggg tctccgtcga
     3421 cgaagagccg gcgacggtgc tcacgtacgc cccccgcgtg gacccggcgc cgctgaccgt
     3481 cagcgtgccg ggtcgcgacc cgacgctcgg tgcactggtc gtcgtgatca cgaacgggac
     3541 gcccgcggac gtgcaggtga gctccgtgac gttcacgatc acggtcggcc agacgggccc
     3601 ggacggacca ccgctgaccc ccaccggaga gggggtcaag tcgctcgtat cggacacgac
     3661 caactggtcg ttcgacggcc ccacgagcca ggtcacgagc ggctcggccg actacgtgct
     3721 cggcccccag accggaagca ccgtgacgct ggcggcgggg gcgtcggtct acgtcgagat
     3781 ctacgacttc cagacggccg agtccccgag cacctccacc gtgacggtcg aggaggtgat
     3841 cgccgacggc gatccgacgt tcacgaacat cggcgtgagc acgttccccg acgggttctt
     3901 cttcgacagc ctcgccgtaa ccgtcccgag cgacaacgcg ctcacaccgg tcgcgcaggt
     3961 ggccggcggg accggcgtga cgctgacctg gaacacgtcg ctcgtcgacg cgaggaacca
     4021 gaccgtctac tggtcgagcc cgaccagcgg gcagcagcag gcgaccccga cgaagctcgg
     4081 cgagtggagc accccgccgg accagcagct cacgagcgac acggtcttcg tggtcgtggt
     4141 cagggcgcag ggcgtccgca acgagtggat gaccgcgtcg ctcgccacgg cggtgtcggt
     4201 cctggatccc gccctcgtgg cgtcgtcggc ctcggtgagc ggcgccctga gcgccgggag
     4261 cgtgcaggcc gcctcggcga ccgtcagcgg cggcttggaa gcgggcacgc tcacggccgg
     4321 gaccggcacc ttcggcgtgc tgcttgccgc gggcagcgcg acggtgaaca tgctcggcgg
     4381 gccggacacg attccgctcc agccgagcgg cccgtacccg tcctttacga actacgtcgc
     4441 gaacaccgac ggaatggtta tcggggcggt gacgtggccg gacatcgacc cctcggtcgg
     4501 taccgcttgg gcgatggcct gcgggaccag cgggcctctc cagctgtata ccggcggcac
     4561 tcggtcggac gacagcggtc ccaagctcgc cggcgattcg ttcgtgcttc ccgtcgcggc
     4621 gggcgcgccg ttcagcctct ggtcgatgct gcaggggagg gccgggtcgg ggtccgcgag
     4681 gttcacctgg atgtcgctgg gatcgccgcc ggcgggccag ccgccctacg agcagatcgg
     4741 gaccgcggag ccggtcgacc tcgccgcgct cggcctaccg aacctcccac cccaagaagg
     4801 gaccgactga cgatgatgat cacccgcccc aacgcctggg tctccgtcga ggacgacccc
     4861 acgacgcagc tcacctacgc cccgcgcgtg gaccccgcgc cgctgaccgt cagcgtgccc
     4921 ggccgcgacc cgacactcgg ctcgctcgtg gtcgtgatca ccaacgggac gccggcggac
     4981 gtggaggtga gctcggtgac gttcacgatc gtggtcggga cgaccggccc ggagggcccg
     5041 ccgctgaccc cgaccaccga gggcgtcaag tcgctcgtct ccgacacgac caactggtcg
     5101 ttcaatgggc cgcccagccc gcgcacgagc ggcacggccg actacgtgct cggcccggcg
     5161 acggggagca ccgtgaagct ggcggcgggg gcgtcggtct acgtcgagat cttcgacttc
     5221 cagaccgtgc aggttccgag cacctccacg atcacggtcg cggaggcgat cggcggcggc
     5281 gatccacagt tcacgaacat cggcgtgagc accttccccg acggcttctt cttcgacggc
     5341 ctcaccgtga acgtcgggag cggcagcgcg ctcgcgccgg ccgcgcaggt ggtgaacggg
     5401 acgggcgtga cgctgacctg gaactcgtcg gtcgtcgaca cgaagaacca ggccgtcctc
     5461 tggtcgagcg ccaccggcgg gcagcagcac gcgattccga cgaagctcgg cgagtggccc
     5521 acgcccgcgg gcctgacgag cgacacggtg ttcgtcgtgc tggtgaccgc gcaggacgtg
     5581 ggcggcgagc cgatgaccgc gtcgctggcg acggcggtgt cggtgcagaa ccccgcgctc
     5641 gtggcgagct cgatccagac cgggcagtcg acggtgagcg gcaacgagac ggtcggggga
     5701 acgctcacgg cgaccggcgg catcgccgcc aacggcgggc tcaccgcgac cgcggcgacc
     5761 gtgaacggtg cgctgaacgc caacggcggg ctgacgacca acgccgccat ggtcaacggc
     5821 gcgctcatgg cgagcggcgg gatcgccgcc aacggcggca tcacggcgag tgccgccgcc
     5881 gtgaacggcg cgctgaccgc cagcggcgtc gtcaacgcca acggcgggct gaatgccacc
     5941 ggcctcagtg tgagcgggga ggcgcgggtc accggcttca ccacgctctc gtacctgaac
     6001 ctgacacgcc tgttcatgaa cacggacgtc atgaactcga tctacgccat caacaactcg
     6061 gcccagtatc cgaccgggta cttccagaac aacgtcggac ggtcgggaaa ccaggtcacc
     6121 ggcgtgtacg ccaaggtcgc ccagggcagc gactgggggc tctacaccaa cgggcgctcg
     6181 ggcagcgaca actcggcggt cacgcacgtg gagacgcgcg acggccaccg cgtggtgacc
     6241 tcgccgctgg tgctcgaggc ggaggtgcag gtgtccggcc aggcgcggct ccgcaagggc
     6301 cgggcgaccg tggagctggg ggcggacggg gcggacatga tcctccacgg cgaggagcac
     6361 tcgtaccgcg tgctggtcac gccgacggcg cgctgcaacg ggctcgccgt cacgtcgaag
     6421 gacgatgcgt gcttcgaggt cgaggagctg ctcgacggcg acagcgacgc ggagttcgac
     6481 tggctcgtga ttggccgcaa gcgcgcggcg ctgggatcct cgcacgccga cgtcctgccg
     6541 gagagcctgc cggtcgtgga ggagccgccg ccgatggacc actagggggt ccgcaggccc
     6601 acgggccgca ggtagttacc gcccggccgg ccctcagaac ggccgcggct gcccgatccg
     6661 gtacgacgtc gcgttctgga acctgttcgc gaggatgtcg ttgccgaagg ccttcgccga
     6721 gtccgtgacc gcgatcggga tccagccgaa cgagtactcc gcgaggtccg cgagaaactc
     6781 ggcctccacc tggtaggggg agtggaactt gatcgggttg gccccgaggt cgatcgtgtg
     6841 cagctggccg aagtcgaacg cgcccgtgcc gtggaagtag aacttgtccc cctcctggcc
     6901 acccgtgatc gccccaccga actcgtggtg ggactgccag ttgtacttgt actgccagtg
     6961 gtgccagccc tcgtggacca tcacggaggc ccggcgcgcc ggatcgtcca cgtacacgcc
     7021 gacgttgaag accgggcagt acatctcggt cctgctgccg gactcgatcg ctttggcgga
     7081 caggccatcg ttgcggttcc tgaactggta ctcgtagtcg tcgtggaagg cgttgtccgc
     7141 gccgttcgcc gcgagcatgt agtcctcgcg actgtggaac tgcgggaggt actcgttccg
     7201 gatcgcgtag gtgagcaggt agatcgagtt gatcatcttg ccgtagggga gccgagcgtc
     7261 gtccggcgcg ccgccgccgg cgggattcca cgagtcgtcc gtgaggtcga acgcggcgcg
     7321 cgcgtagctg tagaactcgg gccactgcgc ctggtagggg atgagccgcc acacggccag
     7381 gtcgaccgcc gcgaccccgt caatgcacac cgtggccccg ctcgtttcgg tgcggcatct
     7441 cgggcgggtg gccgtgctgc gccgcatgct cggcggccag ccgctcgaag tcctcggcgt
     7501 acatgccgtc gaagcgccgc gggaggccga cggcccacgc gtcggccgag cggacacgct
     7561 tggcgagctc cgcagcggca tccgggtcgt cgtcgcggtt gaacaggtac gcgtcgatgg
     7621 ccgccgcccg cacggcgccc agtgggtggt cgaggacgat cttcatcacg cggtcctcct
     7681 gatccgtctt gaggacccac gcgagcatct ccgcggcgcg cgcctcgagg atcccgacgg
     7741 gctcgaagcc gcacccgtcg ccgtcggcgg gctcggggga cggtccgtag atctcctgct
     7801 tgctcatcca caggaagcgc tcgagggcgt ccaccgagga gggctcggcg agctctccga
     7861 tgatcgacag gagaaggccg tggcgcccca cgtccgaggt cgggagcgag tggagctcct
     7921 ggtgcagcac gtcggcgacg gcggaccgct caccccactc gccgatcatc cggcgcacgt
     7981 cgtcgcggcg cccgttcgga gtcgaggcga cggcctcgat gagcgcgctc agcgcctctc
     8041 ggtcgcgcgg cgggggcagg ggcagggtcg gggactcggt cttgggctcg atctcctgcc
     8101 gcgcgggcgg gtacgcgtag gccgaggtct tgcctcgccc gagatccatc tcctccggtc
     8161 ccttcatggg cgcactctcg cggctgaggc atccgcaatg taacccccct agatgggggg
     8221 ttaatgcgca tgaatccgct catgaagtct tgcgcgatca ggtgtaggat ccgacgcgct
     8281 tgcggctctt cactgcgctt cgccgccccg ccctcgccct gttcggcgca gcgctggtcg
     8341 cctgcctcgc cctggccctc gcgagcaccg cgcaggcccg gacggacgac cgcgcgaagg
     8401 acgtctactg gatccacggg ttcagccaca acgagagcat cgactgcgcg aaggagttcg
     8461 cggtcatgaa ggagcgcttc cggtcatggg gcttcacggg ccggtgggcc accgtcggct
     8521 actacacggg cgacaagaac tgcacgatgg ccgagggcca cgggtcgtac cactcgcgcc
     8581 acgccggcgg tcactcgcgc gaccggcaca cggtgaacga cccgctcgag cacatcgcct
     8641 accacctcgc gtggctgatc tacgacctga gcacgaagca cggcaggacg gtcgacgtgg
     8701 tcgcgcactc gatgggcggc ctgctcatcc ggtacgcgat cgcccagacg aggaatcacg
     8761 acccggactt cccgccgtcg ctgaggatcg aggacgtggt gacgatgggc acgccgcacg
     8821 gaggcgtgcg caacagctgg ttgacgatcg gctgcgggac cttcgcgtac cagtgccgcg
     8881 agatgtcggc cgggtcggac ttcctgaagt ggctcgagga cgacgaccgc aacagcaacg
     8941 gctggaatcc gcagtcgagc gtccccacgg actggtcgac cttcggctcc gacgacgacg
     9001 acttcgccgc cgcggaccgc gcggcgggga ccgcgcgaga tcgcagcccg tcccacgact
     9061 acatcggctc gtgccacaag acctggtaca agacggccga cgacatcgag cacggcgatt
     9121 tcaagaccga cgcgagcagc gcgctgaccg ccgatgtcta ccgctacaac tgcaacggcg
     9181 gcggctggag gagcgacccc gactcgcact ggccgatccg acgggcggct cgcgctctga
     9241 cgttggggac cgagtagcaa cggaggagga gacgagcgac atgggaacga tgttcgacac
     9301 ggttggcgac ccgaaagaga ccttctcggg gctcgaggtg gaggccgtcg cggcctatgg
     9361 aaacggcagc tacgccaact acacgagggc gaaggaggag ttcccccacg tgccggtcct
     9421 cgagatcgac gtgagcggcg aggggatcgg ggacgccggc gatttcgagc gcggtgacat
     9481 gtcgtatgcg gatgcggggc cctgggccaa gcgccggatc gccgcggggg tgcaccggcc
     9541 ggtggtctac ttctcggtgt cgaactggca ggcggtgaag cagtcgctca gcgaagccgg
     9601 gatccacccg agcgacgtcc ggctgtggac cgcccactac accggcgagg cccacctctg
     9661 ctcgtcggcg tgcgggttcg gcgacaagcc gaccgatcac gccgacgcca cgcagtggac
     9721 cgacccggac acggcgccgg ctccgtacag gggccgcaac atcgacgtct cggagaccgc
     9781 ggccaacttc ttctgagccg ggtggagggc tggagggcta gcgcgagcgc ggcacgtccc
     9841 tgcccggctc gttcttcccg ccggtctgga cgaacgccac gcagtcgccc tggctactga
     9901 agcccagctc cgcgaagtcc ctgaagccgc ctgccttgca ctcctcaggc gtcgtcggcc
     9961 gcggcggctc cgggtcgggg tccgggtccg ggtcgccctg ggacacgggc ggcgtgccgg
    10021 tcaggaacat ctcggagaag tccgcgaagt tggccggctc gccgggcgcg tcgctgttgt
    10081 agaggctgct gctctcggcc tgtccctcgg tggtgtacgt cccgccggtc gccggccgta
    10141 tcacggcctc gtagttcgtg aggctcgcgc cggaggacac ctggtactgg tccgcctccc
    10201 tgcacgacgg gcccgcgccc gggacgagct cggcgaactc cttcgtcccg gtcacctccc
    10261 cgttgacgga gtcgatggtg aacgtcgcgt ggaaggcgga gagcgagaag ccgttcggcg
    10321 cgttcgggtc cggcaccagg ctgaacgtgc cctgctcctc gaacgtgccg gggtagggac
    10381 cgaaggcggg gccggtcgcc gtgaacgaga acgtcgacgt cccgtccggg gtgcaggtgc
    10441 ccgtggtcgt gaactcgccg ggcgtggcgg ccgccgggaa cgcgaacacc tcgcccgtcg
    10501 ccaccagctg ctcgccggtg aggctcggca cctgcgcggc ggccggtgcg gccgaccaca
    10561 gcgcggtcgc gatgcacgca gccgcgacca atccccgcct acctgcccac atcgctcccc
    10621 ctcgatcgga aattcggacc cgctcaacct aagcgctgtc tcgcggatgc gccagccccc
    10681 ttcgtcccgc gggttcggta gcttcgacgc cgtgagcgcg cccgactcgc agaccacctc
    10741 gacgccggcg ggacaccgtt acgacgcggc gctcgccaac gccatcgagg ccaagtggcg
    10801 cgagcggtgg gcgaccgacg gcgtgttcct ctcgcccaac ccgcgcggcg agctcgccga
    10861 gcccaaccac gggctgaccg agcgcccgaa gctctacgtg ctcgacatgt tcccctaccc
    10921 cagcggggag ggcctgcacg tcggacaccc cctcggctac atcgccacgg acatctacgc
    10981 ccgcttcaag cgaatggccg gcttcaacgt tctccacacg atggggtacg acgcgttcgg
    11041 cctgccggcg gagcggtacg cggtcgacac gggtcagcac cccgaggtca ccacgcgcaa
    11101 caacgtggcg aacatggggc gccagctgca ccggatcggc ctcgggcacg acgaacggcg
    11161 cggggtgtcc acgaccgacg tcgagttcta ccgctggacg cagtgggtgt tcctccagat
    11221 ctacgactcc tggtacgacg aggaggcgga tcgtgcccgg cccgtcgagg agctcgtcgc
    11281 cgagctggac gccggacgcc gcggactgcc cgacgggcgt gactggcggt cgctggacga
    11341 gcgcgagcgg cgcagggcga tcgacgcgca gcggctcgcc tacctcgacg aggtgacggt
    11401 caactggtgc cccgcgctgg gcacggtgct cgccaacgag gaggtgacgg tcgacgggcg
    11461 cagcgaggag gggaacttcc ccgtctaccg gcgtccgctg cgtcagtgga tgctccggat
    11521 caccgcctac gccgaccgcc tggtcgagga cctcgcggag ctcgagtggt ccgactcgct
    11581 gaaggccatg cagaccaact gggtcgggcg cagccccgga gccgtgatca ggttcgggct
    11641 cgaggcaccg ggcgcggacg agctcgaggt cttcaccacc cgccccgaca cgcttttcgg
    11701 agccacgttc gtcgcgatcg cgccggagca tccgctgctc gagggcgacc tcccggcgga
    11761 gacggcggag gcgtgcgcgg cctaccgcga ggaggccaca cgccgcagcg accggcagcg
    11821 acaggtcgag acggagccgt ccggcgtcta caccggcctc gacgccatcc acccgctcac
    11881 gggcgaacgg gtaccgatct acgccgccga ttacgtgctg atccagtacg gcagcggcgc
    11941 ggtcatgggc gtaccggcgc acgacagccg cgaccacgcg ttcgcgcgcg cgttcggcct
    12001 tccgatcccg gtcgtcgtgc agccgccgcc ggactggctc gccgagcacg gcctgtcgga
    12061 ggacaccccc gcggccgagt ggcccgacgc gtacacgggc gccggcacct cggtcaactc
    12121 cggcccggcg ttcgacgggc ggcccaccga ggaggcgcac cggctgatcg tggaagcgct
    12181 ggtcgaggcg ggccgcggcg agcgacgggt cggctacaag ctgcgcgact ggcttttcag
    12241 ccggcagcgg tactggggcg agccgttccc gatcgtctac gacgagcacg gcgacccgca
    12301 tgcgattcca gactcgatgc tgccggtcga gctgcccgag ctcgaggact tcgcgcccgt
    12361 cgtcgacgag gactccacgg ccgagcccga gcctcccctc gcccgcgcgg cggactgggt
    12421 cgaggtcgag ctcgacctcg gggacgggcc caagcgctat cgccgcgaga ccaacacgat
    12481 gccgcagtgg gccggctcct gctggtacta catccgctac gtcgacccgc gcaaccacga
    12541 ggcgttcgcc gaccccgaca acgagcgcta ctggctctcg agcgagggcc agatcggcgg
    12601 cgtcgacctg tacgtcgggg gtgtcgagca cgcggtgctg cacctcctgt acgcgcgctt
    12661 ctggcacaag gtcctgtacg acctcggcca cgtctcgagc cccgagccgt tccagcggct
    12721 gttcaaccag gggatggtcc aggcggcggc ctacaccgac gagcgcggct tctacgtcaa
    12781 cgccgccgag gtcgaggagc gcgacggccg tcacttccac ggcggaggcg aggtcacacg
    12841 ccactacggc aagatgggga agagcctgaa gaacggcgtc tccccggacg agatcatcga
    12901 ctcgtacgga gcggatgcgc tgcggctcta cgagatgttc ctggggccgc tcgaccagaa
    12961 ccggccgtgg gagacgcagg ggatcaccgg cgtcgcgcgg ttcctgcggc gcgtctggcg
    13021 gatggtcgtt gatcctgaga cgggagacgt ggtcgtcggc gagggggcgc cgccggacgc
    13081 gaccctgcgc gcgctccacc ggaccatcgc gaccgtccgc agcggaatcg accggctgca
    13141 cttcaacacc gggatagcgg cgctgatgga gctctcgaac gacctgcgcg acacgtatgc
    13201 cgagggcgcc gtgccgcgcc aggtggcgga gtggttcgtg ctcgcgctcg cgccgttcgc
    13261 tccgcacatg tgcgaggagc tgtgggagcg gctcggccac accacctcgc tcgtctacgc
    13321 aacgtacccc gatcccgacc cggcgctcct cgagcgcgag gtggtcacga tcgcggtgca
    13381 ggtgaacggc aagttgcgcg gctcgttcga cgcggcgccg aacaccgacc gcgagagcct
    13441 cgaggagacg gcgcgtgggc tgccgaacgt ggcctcgcac ctgagcggtc gcaccgtgaa
    13501 gcgggccgtg gtcgtgcccg ggcggctcgt gaacttcgta acggactgac gccgcgcacg
    13561 tcacagtcgg cccccgcggg gcctctcaac ggttgacacg cagatcgacc cggaggagag
    13621 acgcggatgg acacgaccca aggcgcagtg ctcgagttgg ttgtcttcaa gctgaaggac
    13681 ggcgctgccc gcgagcagct gctcgggacg gtcgacgcgg tgtccgcctg ggtggcggag
    13741 cagccgggct tcgtcgggcg cgagctggcc cacgaccgag gcggcgaccg ttggatcgac
    13801 atcgtccgct ggcggagcct cgaggacgcc cacgccgccg ccgagctggc gatgagctcg
    13861 gaatcgtgtg cgccgatgtt cggcctgatc aacatggagt cgatgctgat gctccacgcc
    13921 gagccggcga tcgcaccggt ccacgcgtga cggcgcggcg cgcgctcgag gccgaggaga
    13981 tcctgagcga gctcctcggc ctcgacccgg gcctcacgcg cgagcgctac tggggcgagg
    14041 acgcggtctt ctacaacccg ggccgagcgg ccccgctcgg cgtcatcctc gccgcgatca
    14101 aagaccacga cgggccgaac gaccgatcgg cgcagctcga tcggcccggc gtctaccgct
    14161 tcgccttcgg catggccccc gactcctacg agcgccgctt cggcgcgcac ccgggccggc
    14221 cgccgaaggg cggcgtgatc gacctgcccc actacgaccc ggcgcgggtg gggcggctca
    14281 tgccgcatcc ggtctacggc tggatgagct gggtgcagat cctggcgccc accgcgcgca
    14341 cgttcgcgcc gctgcgcccg ctcctgctcg agtcgctgga gatcgcgcgc gcgaagtgga
    14401 gccgccgcgc gtaatctcga ggcgatgccc ctgccgcccg acatcgacag cctggtcgcc
    14461 cgcgcgcagc ggggcgacgc cgcggcgctc gaggcggtgt gcgcccgcgc gcgcccgctt
    14521 ctcgttcggc acgcgcgccg gctactcggg cgctccgcgg acgccgagga cgccgtgcag
    14581 gaggtgctca tcctgacctg cgcccggatc gggacctacc gctgggaggg cagcttcgcc
    14641 ggctggctct acgcgatcgc cacccgcagc atcctgcgtc gcgccgcccg cacgccacgc
    14701 gacgccgccc tcgccgcgga gctcgccgac cgcgccctcg tgtcggatta cgagccgatg
    14761 accgaggccg agtggcggct ggtcgagcag gacgtgcaca tggcgtgcac cgtcggcgtg
    14821 ctcaccgagc tgtcgcccga gacgcgcagg ctgtacctgc tcggcgaggt cctggcggtc
    14881 ccggaccgcg tgggcgccga ggtggcgcgg acgacgcccg ccgcctaccg caagcgcctg
    14941 gagcgcgcgc gccgagcggt ggccgccgcg gtccgtgccc atgcccaacc ctccgaggac
    15001 gacggccgcg tggccgccgc cgcccgcgag ctggacgacc tgctccgcct cggtgagctc
    15061 caccgtgccc acgcccgccc cggcacgccg gccggcgctc tgcgcgcgct cgagcgcgtg
    15121 gcgccgacgc tggcgagctg agcggccgcg ggctaagcgg gtcgcagaag cgcctcggcc
    15181 agcgttctga ggatgggggc accggtgcga gtctcgatcc acccgaactg cccggccggg
    15241 ttgagatcca ggaagtacgt cttgccgtcg tgcgtacagg cgaggtcgat cgccgcgtac
    15301 cggagtcccg ccgcgtccac gagcgctcgg atccggtcga cctcgtccgg agcggcctct
    15361 acgagctcgt gggagaccgc gttcacgtcc cgacgccaat cgacctcggt ccgggcgctc
    15421 gcctgtgagt cgatccggca gacgaacacg gtgcggtcga cgacggtgac tcgcagctcg
    15481 catgccttct cgagatacgg ctgcaccagc acgggcgctc tcgtcagccc ggtgcggctg
    15541 ctgtcgagct cggcgctcgt gcaccgttgc gtgtaggcga cagccatggc cccgtcgggc
    15601 gtttcacgcc acgcgccgcc ctgggacttg acagcgacct ggtcgaactg ctgagcgaag
    15661 cggacgatcc tgtcggggtc gttggagagc agcgtcggtg ggacgtcgaa gcccgcacgg
    15721 gcggcgagct tcatctgcgc cagcttgttc ccgtccaacc agctcgccct caggggatgg
    15781 ttgatccaga tcgattcggg ggcggtcgcc gccaggccgc ggagcgtcag ctcccactcg
    15841 tggcggacgt accgagctgt ggacgcgtcg tcgtcgcttg caagtggcgg ctcggcgtgc
    15901 cagttccaga tgccccggca cgcgtcgagc cggatgggat cgccgccgag cctgatctcc
    15961 gcgccacacg cgtcgagcgc cacgcagccc tcacgcggaa gccgccttgt cgagagtacg
    16021 tgcgcctggc cgccgagcga cgcaacacat tcgacgagag aagcgacggt gggcgagccc
    16081 tcgtcgctcg cgacgatgac ttgaggcgac tgcgcccggc tcaatccttg caggcgtagt
    16141 cccaggcgac gatgcgcttg ccgtcggcgt gctcgaccgt cgaatagccg ccgcaaccgg
    16201 ttccgccctg cgcctcgagg tgcgagacga actccgcctt gctcatcaga aacggctccg
    16261 ccgcggtcgc ggattcgatc ccggtggcga tctcgtcgct cgaacgctct gccatcagtc
    16321 agtccttggt ccggggatga attttcagct tacctcggca gggcccgcct cgcaggtggg
    16381 gaggctcggc gctcagcgtg ggcggtcgcg gtcgcgcgag ggctggacgc gcttcggctc
    16441 gccgggcatc ttcgggtagt ccggcgggta cggcatgtcg gcctcgccct cggcctcgtc
    16501 gcgctcgtac atctcgagca gcggctccag cgagtgggcg gcatcgtcga tcgcggcgtg
    16561 gaggtccccc accgcggcga agcgcgccgg catcgtcgcg accgtgaagt cctccggcgc
    16621 gacctccggc agctcgtccc aggtcaccgg ggccgagacc ggcgcacccg gcttcgggcg
    16681 gatgctgtag gccgaggcga tggtccgatc gcgggcgttc tggttgtagt cgacgaagat
    16741 gcgctcgccg cgctcctcct tccaccactt ggtggtcacc tcgcccggca ggcggcgctc
    16801 gagctcgcgt ccgaacgcga tcgcggcgtg gcggacgtcg gtgaaggtcc agcgcggctc
    16861 gatccggacg tagatgtgca ccccgcggcc acccgaggtc ttggggaagc ccacgtagcc
    16921 gaggtcgtcg aggagcgcgc gcgccgtggc cgcgacgcgc acggcgtcgt cgaagtccgt
    16981 ccccggctgc gggtcgaggt cgaggcgcag ctcgtccggg tggtcgacgt cgtcgcttcg
    17041 gaccggccag ggatggaagg tgatcgtgcc catctgggcg gcccacccga cgaccgccac
    17101 ctcggtcggg cagatctcgt cggcgtgacg gccggacggg aactggatgc gggcggtctc
    17161 gacgtagtcc ggagcgccct tcgggatgcg cttctggaag aaggcgtcgc cgccgccctt
    17221 ctcgcgcgtc gacaggacca tcccggggtg cacacccttg ggccagcgct cgagcgtggt
    17281 cgggcgccgc gcgagcgcgc gcatgatgcc gtccccgacc gcgacgtagt agttgaccac
    17341 gtcgagcttc gtgagagcgg gcgaacgctc ggtcgcgggg aagagcacac ggtcgggact
    17401 ggtgacgcgc aggtcacggc caccggcttc cacggtcgtt gcgtcgggca cggggggagc
    17461 gtaggggaag gcaagcgcgc ccccacggct caaaatacgt gtgggtcagc cgatagtctg
    17521 ccaagcgacg tatcggcgag caggggcaag tgcgtccagc gcatccgccg gcggagcagc
    17581 ccacccaaga gacctacggc catgaccgag cctgtcacct ccgcaatgcc catcggattc
    17641 atcctgcgag ccgcgacgcg cgacgcgcag gccgcggctc aggccgccct cgatccggcg
    17701 ggcatcacgc tgcgcgaata cggcgcgctg acggtgctgc gcgaggatgg gccgctgtcg
    17761 cagcaggagc tcgcgcagcg gctgggcatc gaccggacct cgacggtgtt cctcatcgat
    17821 cgcctcgagg agctgaagct cgtgaagcgg cgacgcagtc cggccgaccg ccgggcgtac
    17881 gcactcgagc tgacccgcac gggcacgcgc gtccagggcc gcgctcaggc agcgatggcc
    17941 gagcaggaga gcaggttcct gtcggggttg acgcagcggg agcgccagca gctggtcgat
    18001 ctgctcatgc ggctggtcga ggcgcccagc gactgagcgc ccccggccag ccgcgtctca
    18061 cagccggcgc cagcgcacca gcagcccctc gagcgccgcg acctccgcga gccgcctgcc
    18121 gagcccgcgc agcagcggcg ggcgcgcacc caggggcggc tcggcgctcg ggtgagcttc
    18181 ggccgccggc tcgcacccga tccgctcgcc cgccatcgcc agcagccaga gggtcgtgcc
    18241 cggcgtcacc tgccgcgctt ccccggcggc accctcgtca ccgcccgagg gcccatccgc
    18301 gggccgctcc ggcccggact gcgtcatccg cctgttggtc atgatgacct cccttccgtg
    18361 gtcacgctct gcggagagcc gatccggact cggttctggc ggcgtcgtcg tcgcccaccg
    18421 attccgcttc gtcgagggcg tgcccgagcg cgtccacggc gaagtcgagc tcggcgtcgg
    18481 tcgccaccat ggggggcagc agccgaatcg tctcgggcgc gctgaggcac ggggacacga
    18541 gcagcccctg ctcggcgaga tcgacgagca cggtgccggc cagcccggcg ctgcgtagct
    18601 ggatccccca gaggagcccc cgtcccctga cctccgacac gaccgcatgg cggctggccg
    18661 cgagatcgtc gagctcccgc tcgcaacgtg ccgcgatctc tcgcgcgcgc accgccgcgg
    18721 cgtcgatcgc gtcgagcgct gcggtgccgg cggcgcaggc cagcgggtgc ccgccgaagg
    18781 tggccgtgtg ccaggtcggg tcgcgcgcga gcgggctgta gaggtcgtcg gtcgcgacca
    18841 tggccgagag cggcagcacc cctccgccga gagccttccc gagcaggacg gcgtcgggct
    18901 cggcgccctc ggcccgcgcg atcgagaacg ggccgcacct gcgcagccca cactggatct
    18961 cgtcgctgat cacgtacgcg ccggcgtcgt gggccgcggc ggaccagcgc cgcatcacgt
    19021 ccggctccag gcggcgcacg ccgccctcgc cctgcaccgg ctcgaatacg agggcggcga
    19081 cgtcacctcg ctcgagctcc gcctcgactc ccgcgacgtc gtcgcgtccc acgcgggtga
    19141 cgtggccgag cagcggctcg agaccggtgc ggaacgctgg gctcgcggtg agcgcgaggg
    19201 ctcccagcgt cttcccgtgg aaggccccct gcagggctag caccctcagg cggccggtcc
    19261 gccgccgggc cagcttgacc gcgacctcga ccgcatcggc gccgtcggtg ccgatccaga
    19321 gtcgcgggaa agcgccgtcg aagcggtcga gcagcgccac ggcgaaggcc gccacggcgg
    19381 gattcgcgag cgttcgcgtg gccgtcggca tcgacccgag ctggcgctcc acggcgcgca
    19441 cgaccgccgg gtggcggtgg ccgagcagcg tgacaccgta cgacccgaag tcgaccgccc
    19501 gccgcccgtc cgacagcgtg acggtcgccc cgtcggcctc gacctcgacg gcgtccctcc
    19561 ccgccatctt gtagccgagc gcgagtccgg gggagagatg ccgcctgatc gcgtctatga
    19621 cccagccgtc gctcgccggc tccgaccgcc gccccgatgg cggcggcgcc gtgggggcca
    19681 gttcggtcac gcgcccacgg accctctacc gcggcgccgg cgccgggcgc tcgagcgccg
    19741 tgtgctcgac cccccgcagc atctcagcgc agttgtcgcg cagcgcgcgc tgcgcgaccg
    19801 ggttcagcat gtccgcgagc agcgggatgc cgatgtcgaa ggagacgcgc aacctcacgc
    19861 tcgtcagcgc gtcgccctcg ggggtcacca cccactcgcc gtccagcctc tccatgtcgc
    19921 cggcgacctg ctcgaaggtc atggtcaggc gctgcgagtc gatcgtctcc acctcctccc
    19981 actcgaggat cgatcccttc agcagcaccg accacacgct ccgccgcttc ccctcgcgtt
    20041 cgtcgtccag gatctcgatc gagcgcacgt tctccatcga cgcggggtag cggtcgatgg
    20101 cgacgatggc gtcccacacg accgccgcgg gggcgcggat ctccatgtcc agcgcgacct
    20161 caggcacggg cctctccctg ctggctccgc gtccactcct tggggaggaa gccctcctcg
    20221 cccatcgcgt cgatgaacgt cagccagtcg cgcgcgaagg cgagggccgg gtagtagccc
    20281 tccgggctga gtggatcggg gatctcgccg acgacctcgc ggacgcgctc gaccaggcgc
    20341 tcggaccggg cgacgagctc ggcgggcgtc agctcgtagc tcgagcgcac gagctcccag
    20401 cactcccccc cgagctcgtg cccgctcagg cgctcgagcg cccacgcgac gcgcatccgt
    20461 ggcggcggca gcggccgctc gtggttcagc accatgttga ggacgacggc gcagttctgc
    20521 cccaccgtgc gggccgtatg gcggacgaga ccgtcctgct cggcggccag cccgccgcgc
    20581 atgtcctcga gcagggcgac gatcggcagg cccatccgcc acatcgcgtg cccgtgagtc
    20641 cacgcgtacc gccgcgcgtc gacgtcgatc gtcctcacga cccctccccc acgtgcgtca
    20701 gctcggccgc ggtgaccccg cgcgaccaga ccatcgccac gacgccgcgc tcgtagagct
    20761 ccaccgcgag gctcgcctcg gcggggtcgc attccgcggc gggccacggg cccccctcgc
    20821 cgtagcggtc gacgagccgc ccccaggcgg cggcctccgc ctcctcgatc cgccacacca
    20881 tgccgggctc ggtcgccacg agaacccgct cgccgccgag cgcgaacgag cgtagccccg
    20941 cgggcgacca cgcgaacagc ggcgcgaggc gctccggcgc cagctccatg ccggtcatgc
    21001 gctcggccag ctccgtggcg ccggcgacga agccgcccac gtcgctc
//
LOCUS       DASVPL010000322        18210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_154693, whole genome shotgun sequence.
ACCESSION   DASVPL010000322 DASVPL010000000
VERSION     DASVPL010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18210
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154693"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VF517_18505"
     CDS             <1..853
                     /locus_tag="VF517_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054981.1"
                     /translation="VTLVAGNHDHQLAAPLLDAHRLDGAAPLALDAAAPPPATGPVAS
                     IAEWLGPAELRLAYPGTWIRDDVYATHGHYLDLHNTVPALECVAVSAVERVLGRSEDG
                     RRTPDDYEAALAPFYALAFELAQARKTTRQTTGGASVAIWRRTGGAGSPTTLTGRLLA
                     DVAIPAAVGALNRTGIGPYRPQLDGPALRRAGLRGIAEVTSHLGVDAPYVLFGHTHRS
                     GPWPGDDASEWSLPSGGRLLNTGSWIFDEAFGTAGPYVPGTCVFVDADGPPRLERLLG
                     EETATRT"
     gene            complement(840..2357)
                     /locus_tag="VF517_18510"
     CDS             complement(840..2357)
                     /locus_tag="VF517_18510"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8054982.1"
                     /translation="MWVTIGSGEGEGLSVRVEGERFLLGSGEECQLMVRGGDVRPLHA
                     YFQVRPDGVVELHPLEGETYVNGHLLSGPAHITGGEEIRIGDSLLKPSVDDPAEEARA
                     LAGAEGEGPAEPAPVVRVETEGQTVEVVPGAGSDGGPEDPVSKVRVTTEGEAVEVVPA
                     RERRRMLRMTRRATMLAAGAAVLGLAALLAVLLLRGDDEKSVAQIVRDAKSQTVLVKV
                     QAAQGDGAGSGVVIDAGEGYVITNYHVVNGAQKLQVAVDEDSRDAKVVAAAPCDDLAL
                     IKVDDNEGMKSMELASQDDIKQGDEVVALGFPANASLQDNLASTAGTVSVVKSSFNLP
                     DPQAPSLDNVVQIDVALSPGNSGGPLVNKEGKLVGVNTAILTQLGGAPIQGQGYAIGV
                     DRLKEVAEDLRDEKSQGWPGLALIVPKKAELDKLKLPEGIVAGSPSPGSDAAAQHLEE
                     VLITEIDGNAVTANMTSYCRAVEDVESGQRVPITLIEAPSKGGAARERKTRLKFE"
     gene            complement(2413..4617)
                     /locus_tag="VF517_18515"
     CDS             complement(2413..4617)
                     /locus_tag="VF517_18515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEX8054983.1"
                     /translation="MGKTLVIAEKPSVGRDLAAALPGSFKNSKDKTYIEGDDYVITWA
                     VGHLVGLAAPDEYDPKLKKWRFADLPILPDKFKLVPNDERSRKQLKAVHGLIAREDVD
                     RIVNACDAGREGELIFAYTYETSGIEKPVQRLWLSSMTRKAIEEAFARLRPGEEMHSL
                     EDAARSRSEADWVVGMNATRAASIRLRAAFDGAVSLGRVQTPTLALVARREEEIRAFK
                     PEPYWLVEAAFLADPDRAYRGRYLGGKRIDEELAARIVAEAREGTGEITKLEKKEERE
                     RPQLLYDLTSLQRHGNTLFGFSARRTLAAAQRLYEEHKAITYPRTSSRYLPSDQIEEI
                     KPTAELVGRNDQYRRAAEYVVSLDKLPLGRVVNDDKVDDHHALIPTKSEHDLERMGSD
                     ELKIYDLVTKRFLSVFHPEAVFERTRVETTVAEHVFRTSGRRLLEAGWKAVYGAEVEG
                     ERADDDTGGDQLLPKLDQGESVKVTEMESLRKETQPPRRFTEASLLGAMETAGKDIED
                     AELREAMKESGIGTPATRAAIIERLIDVGYIERDGRALVATEKGIQVIRLLGSHPLTS
                     PELTGDWERRLGLIEQGEDSRPSFMSDIAKFATETVAELDKLKDVKIERANLGPCPVC
                     GRDVIENRKGYSCWSREDPGCGFVIWKKKAGKILPASVVKELMATNKTEKQVTGFRGR
                     SGRTFRAKLKLEQGDDGKWRVEFDEEWAKEPRAKPPETEADETATAETPTAA"
     gene            complement(4673..5479)
                     /locus_tag="VF517_18520"
     CDS             complement(4673..5479)
                     /locus_tag="VF517_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054984.1"
                     /translation="MRTPLALLSACVLALGLATAAQGQGGTPVPGAGVIDNPCLGLDA
                     AELLCPNLRMQPPSDLMVSKTRSGRRLLHATNSIDSRGEGPAELRGKRYGKHYMEANQ
                     RILLRGGGRRTVETPARLGFKNIPGQGGYWKYKDAGRFELWTLDENDNPVERVRTGPK
                     QYYCLRDLVRTQPMAGSPRGRVYPACNQNRHANAVTLGTSVGWSDVYPSTYHEQYIEI
                     TGLTGRYGFIHVADPGNGIWENDEDDNAAMTIVSLPSGKRLGSRGPMPRP"
     gene            complement(5609..6757)
                     /locus_tag="VF517_18525"
     CDS             complement(5609..6757)
                     /locus_tag="VF517_18525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX8054985.1"
                     /translation="MTAIDWIIVAFTVLMAVWGYAQGLLVGALSLAGFVGGGFVGSRL
                     GPLLLDDGSHSPYAPVFALVGAFLIGGVLASVLETVGFQLRRRVRGGLGIVADGVGGA
                     LLLACAGLGLAWMLGAIALQTPGAREFRRDIQRSAILTRLNDAFPPRSLLNTLARFDP
                     SPTFRGPAPEVAPPTSRIARDPDIRRAARSTVKILGTACGLGVEGSGWVAGDGIVVTN
                     AHVVAGQDDTTVQLGGEGPRHDADAIWYDSRNDLAILRSTGLAGTPALTLNEDAEVGA
                     SAAVVGYPGNGPLSIRAARLGATTTVLSQDSYGRGPVRRRVTALRGVVRSGNSGGPLV
                     DARGRVLATVFASTTGGGPRGGYGVPDSIVREGLARANERVDTGPCTH"
     gene            6869..8494
                     /locus_tag="VF517_18530"
     CDS             6869..8494
                     /locus_tag="VF517_18530"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX8054986.1"
                     /translation="MPLLAAAATVMLLLAVLNASCGASEEEVTAGRFATAWERGDWEA
                     MHRQLTPAAQRRFPPDALRRAYEAAAATATSTAVTTGEPRGDGDGAVVEVTARTRVFG
                     PVRGELRLPVEDGRVDWAPHLAFPGLKEGELLTRRSEPPRRAPILARDGTRIVTGPGE
                     QRAIVFVPAAGIAGEVAAAAPGPEREAVYARGFPRTWPIGRNGLERAVEDRVAGRPGG
                     TLLAGPRPIARAAPRPAAAVRTTIDPGVQAAAVQALAGRLGGIVALDARRAEVRALAG
                     LAYSAPQPPGSTFKIVTASAALEARRVKAKTRFPVSTRAVIEGVDLENANGESCGGTF
                     ENSFVHSCNSVFAPLGVRVGAGPLVAMAERFGFNRPPPLAGATQSTLPQAEAIGSDLD
                     VGSTAIGQGKVLATPLTLAQVAQTIAGGGVQHTPTVVRGAPAARPRRVVSARTARTVR
                     RFMVGVVRSGTGTAAAIPGTVVAGKTGTAELGTTQGDTGADAGSTDTDAWFAAFAPAR
                     KPRLAVAVMLVRAGAGGQTAAPAARVVLQAGLD"
     gene            complement(8510..9025)
                     /locus_tag="VF517_18535"
     CDS             complement(8510..9025)
                     /locus_tag="VF517_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054987.1"
                     /translation="MALATAASGCQREAPQDEPRREGLGTEVAGLKYTVYITRQINQH
                     LASDRGFYNGPEPKPGFTYYGVFMRVCNESAGFKRPVEHFVVKDSQGNEFEPLEVEPD
                     NVFAYRARRLSKHACVPEAGSPAATGPTAGALLIFEFPVQTLENRPLELEIEETTPEG
                     KVEKGIIELDL"
     gene            complement(9084..9662)
                     /locus_tag="VF517_18540"
     CDS             complement(9084..9662)
                     /locus_tag="VF517_18540"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8054988.1"
                     /translation="MVPLTVHTAIAAPREEIFDFVADLANRTAWMDHWQSELRLTHPR
                     SSGVGAAARYRLSQRNRQWAETHIVEADRPRRIVEATRGGRLNLTLGEIVFEFSRQGR
                     TLTRVEMTVWTEPGTVRERFFARFGAKSQAKRQAKVALQRLRAVFEEQREEPLARATV
                     AGWEPQKAPRFGVELADQPDVHTGSGRRASSG"
     gene            9734..9979
                     /locus_tag="VF517_18545"
     CDS             9734..9979
                     /locus_tag="VF517_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054989.1"
                     /translation="MPRDIASILLRNSLGALHTDRPSCSRCRRLPLTGELMHELESGR
                     EVCSLCLSQSDGRDGEPVRSERVRATDKPLSVVPRAA"
     gene            10111..10731
                     /locus_tag="VF517_18550"
     CDS             10111..10731
                     /locus_tag="VF517_18550"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8054990.1"
                     /translation="MGFVIIRIIFGGETDTVHAGTLAGAGCFRCDSCGYAVALHELDE
                     VPACPHCEGRDFRRSSIFAELSLAEPNGSMDDRPAWLAEAREALVTRGDYIAYDDGDR
                     VRVVALQDGWTRIGRSLAAHVRFDDPTVSRRHALLHRQDEGARILDDRSLNGVFVNGA
                     RVDMRELEDGDQISIGRFSLHFMRLGAGAKPAANEAQPDRDQVAVG"
     gene            10909..11700
                     /locus_tag="VF517_18555"
     CDS             10909..11700
                     /locus_tag="VF517_18555"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX8054991.1"
                     /translation="MATTIAVLSQKGGTGKTTTTRTLTDVLGRIGLDVLAVDLDPQGN
                     LSDYFDVPADADPTIADVLGGKVKAADAVHNGVIPANLGLAEAELILSGKMGREMTLR
                     RALRDLGRRHDVILVDCPPALGLLTINAVVAADYALISTEAQYFSLQGVEQALEIIEL
                     AKENLHPDLEWLGVVLNIADMRTVHSREALAQLKERFGEKLFDAVIRQSIRYAESAER
                     GVSIIDYRPELGDDYLALAGEVLARIGMTEESGRVAELRAELVPA"
     gene            11820..12257
                     /locus_tag="VF517_18560"
     CDS             11820..12257
                     /locus_tag="VF517_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054992.1"
                     /translation="MKRWALAVSVGAIVGAFGAVDGAAAACSTDARDRAASPSGTTVG
                     AAGQSAWVNPSTSAGEVGTSTSAGSNEGGGGLSGGSAYYSGGDAVTGSYTEGSGSGSA
                     STLSGGASSDGAAGGISSSGDLSGSPAGLAVDDVQAGAVCATP"
     gene            12635..13009
                     /locus_tag="VF517_18565"
                     /pseudo
     CDS             12635..13009
                     /locus_tag="VF517_18565"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            complement(13036..13494)
                     /locus_tag="VF517_18570"
     CDS             complement(13036..13494)
                     /locus_tag="VF517_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054993.1"
                     /translation="MAELPQNFLEMLDEANSIVTADYPQAELFEASINLTLLGAPWQF
                     VFGDPATSPASTVILKNFEGQFQTPPQYIDSPWGDDNAIPLPITLDLQEALSLCEQKG
                     CGSGNPDYITLRWPLYPGDTEPFYIVTWVSANQRCFVGVNTQNVQCDAVN"
     gene            13595..15373
                     /locus_tag="VF517_18575"
     CDS             13595..15373
                     /locus_tag="VF517_18575"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX8054994.1"
                     /translation="MREDVRIGCWAGFWGDTREAAARIVRGAPIDYLVADHLAEITMA
                     LLARARARGPAAGYVADAVEALAPLLGEIHERSIRVVTNAGGLDPRGCVDALRAAAAA
                     AGVSMRVACVTGDDVLPLAGTLRERGAELPEQPLTMNAYLGARPIATALDMGADVVVT
                     GRCADSAVVLGPLLHEFGWGDGDHDLLSAGSLVGHIVECGPQCLGGLFTDWEEVPGWD
                     DMGHPIAECRPDGSATITKPPGTGGMVTPATVAEQILYEIGDPAAYVMPDVVCDWRDV
                     RLEQEGHDRVRVTGARGRAATATYKATATRLDGFRLVASAMFAGGDAAGRARRVGEAI
                     LVRAGRIAAEAGHGPFTETSVEVVGAGEAVVKIAARHPERAALEVLAREIAPMGLVAQ
                     GMTGLFAGRPRPAPVVSLTHVLVPKADVAVTVELDGETVAVRPAATPEGAAAKPERIA
                     PDRAAPARAAPEPAPLPEPPPPAVADPVVVPLRTIAYARSGDKGNDANIGVIARRPEL
                     ADWIRDQVTAARVAEVFGRWLDGPVRRYALPGLDAINIVLSDVLGGSGGTSSLRFDPQ
                     GKTYAAILLDMPVEVPADLLARSSGE"
     gene            15387..16607
                     /locus_tag="VF517_18580"
     CDS             15387..16607
                     /locus_tag="VF517_18580"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8054995.1"
                     /translation="MEASDAPDRSSIGLCFSGGGFRASFYALGIMRYLAEARLLDRVS
                     AISAISGGSIAAAAAADRWTEFQTAGGGVEAFLEKIDGPFRSTVTSKNITRRWLIASL
                     AGVIPGTGGRGGAYARTLAHNLYEHDRVADLPREPEFIFTSTDMSKGRAFRIAPGFVG
                     SWDYDYVEPAPRSISLGTAVAASAAFPPSLTVVDVRTKGLGFPKPAPPVLSLVDGGVY
                     DNLGLEWFQGEGSRRPASAKEKPPFTIVANASGLFAVKNKRFWSLTSLGRDLSIQYEQ
                     SLSVRIRWWVETLQEGEPGVYVATKTDPRKEPHLDPAVVRAALPSELVLPLARLRTDL
                     DRFSTEEADLLSYHAYWTVHARLRTYAADLALAEPAWTDYADLSAAETERLRKLLELG
                     SHRFFRRVRAALRR"
     gene            complement(16621..17478)
                     /gene="thrB"
                     /locus_tag="VF517_18585"
     CDS             complement(16621..17478)
                     /gene="thrB"
                     /locus_tag="VF517_18585"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX8054996.1"
                     /translation="MKRRRLVRVPASSANLGPGYDALATALGIYLELEVEETGGELAV
                     ECDVPGVPLGPENLCVRAFQALRDDVGGLTFRIRSEIPLGAGLGSSAAAIVAGLVAAD
                     SMYELDAPLFELAAALEGHPDNVAAALMGGFVICAGDTPARFDPAPGLEGVLAIPPDP
                     VPTEDARAALPDEVPVADAVHNIAHASLLILGLTRGDLSQVGRGLSDRVHQPRRAALY
                     PRTMELVGRAAELGAVGATVSGAGPAVLFWCHWQATGTVLDAVRTAAPDCEVRRAQFV
                     PGGADVRAL"
     gene            complement(17475..>18210)
                     /gene="thrC"
                     /locus_tag="VF517_18590"
     CDS             complement(17475..>18210)
                     /gene="thrC"
                     /locus_tag="VF517_18590"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX8054997.1"
                     /translation="AARAGLRGAVIVPEGKIATGKLAQALMHGARVVALRGNFDEALR
                     LVRELVDRNPIALVNSVNPYRIEGQKTGAFEVCDQLGEAPDVLCIPVGNAGNVTAWWK
                     GFGEYGVAPRLHGYQAEGAAPLVNGAPVENPETVASAIRIGNPARWEEAMDAFTSSRG
                     EVRAVSDDEILAAYALLGEREGVFCEPASAASVAGLLKYGAEGRVVCVLTGHGLKDPT
                     TALARATPVVPCEPRIDDVERAVLGS"
BASE COUNT         2350 a         6761 c         6555 g         2544 t
ORIGIN      
        1 ggtcacgctc gtggcgggca accacgacca ccagctcgcc gccccgctgc tcgacgcgca
       61 ccgcctcgac ggcgcggccc cgctggcgct ggacgccgcg gcaccgccgc ccgccaccgg
      121 ccccgtcgcc tcgatcgccg agtggctcgg gcccgcggag ctgcggctcg cctaccccgg
      181 cacctggatc cgcgacgacg tctacgccac ccacggccac tatctcgacc tccacaacac
      241 cgtccccgcg ctcgagtgcg tcgcggtgtc cgcggtcgag cgcgtgctcg gtcgctcgga
      301 ggacgggcgg cgaacgccgg acgactacga ggccgcgctc gcgccgttct acgcgctggc
      361 gttcgagctg gcccaggcgc gcaagacgac ccgccagacg accggcggcg cgtcggttgc
      421 gatctggcgg cgcaccggcg gcgccggcag ccccaccacg ctcaccggcc ggctgctcgc
      481 ggacgtcgcc atcccagcgg ccgtcggggc tctcaaccgc accgggatcg gcccgtaccg
      541 gccccagctc gacgggccgg ccctgcgacg cgccgggctg cgcgggatcg ccgaggtgac
      601 gagccatctc ggggtcgacg ccccgtacgt gctgttcggc cacacgcacc gctccggccc
      661 gtggcccggc gacgacgcga gcgagtggtc gctgcccagc ggcgggcgcc tgctcaacac
      721 cggtagctgg atcttcgacg aggccttcgg cacggccggc ccctacgtcc cgggcacgtg
      781 cgtgttcgtg gacgccgacg gccccccgcg cctcgagcgc ctgctgggcg aggaaaccgc
      841 tactcgaact tgagccgggt cttgcgctcc ctggccgcgc cgcccttgct gggcgcttcg
      901 atcagggtga tcggcacgcg ctgcccgctc tcgacgtcct ccaccgcgcg gcagtagctc
      961 gtcatgttgg cggtcacggc gttgccgtcg atctcggtga tcagaacctc ctcgaggtgc
     1021 tgcgccgccg cgtcggagcc cgggctcggc gagcccgcca cgatcccctc ggggagcttc
     1081 agcttgtcga gctccgcctt cttggggacg atcagcgcga gcccgggcca gccctgcgac
     1141 ttctcgtcgc gcaggtcctc agcgacctcc ttgaggcggt cgacgccgat ggcgtagccc
     1201 tgcccctgga tcggcgcgcc gccgagctgg gtgaggatcg ccgtgttcac gccgacgagc
     1261 ttgccctcct tgttcacgag cggcccgccc gagttgcccg ggctgagcgc gacgtcgatc
     1321 tgcacgacgt tgtcgagcga cggcgcctgc gggtcgggca ggttgaacga gctcttcacg
     1381 accgacacgg tcccggcggt gctcgcgagg ttgtcctgga ggctcgcgtt cgcggggaag
     1441 ccgagcgcga ccacctcgtc gccctgcttg atgtcgtcct gcgacgcgag ctccatcgac
     1501 ttcatgccct cgttgtcgtc caccttgatc agcgccaggt cgtcacacgg tgcggccgcc
     1561 accaccttcg cgtcgcgcga gtcctcgtcg accgccacct ggagcttctg cgcgccgttg
     1621 accacgtggt aattggtgat cacgtacccc tcgccggcgt cgatcaccac gccgcttccg
     1681 gcaccatcgc cctgcgcggc ctggaccttc acgagcaccg tctgcgactt cgcgtccctc
     1741 acgatctggg ccacgctctt ctcgtcgtcg cccctcagca gcagcaccgc gagcagcgcg
     1801 gccaggccga gcacggccgc gccggccgcc agcatcgtcg cgcgccgcgt catccgcagc
     1861 atgcgccggc gctcgcgggc gggcacgacc tcgaccgcct cgccctcggt ggtgacgcgg
     1921 accttcgaga ccgggtcctc cgggccgccg tcgctgcccg caccgggcac gacctcgacg
     1981 gtctgcccct cggtctccac gcgcacgacc ggcgccggct cggccggccc ctcgccctcc
     2041 gcgccggcca gcgcgcgcgc ctcctcggcc gggtcgtcca cgcttggctt gaggagcgag
     2101 tcgccgatcc ggatctcctc gccgccggtg atgtgggcgg gcccgctgag cagatgcccg
     2161 ttgacgtagg tctcgccctc gagcgggtgc agctcgacca ccccgtcggg gcgcacctgg
     2221 aagtaggcgt gcagcggcct gacatccccg ccgcggacca tgagctgaca ctcctcgccg
     2281 gaccccagca ggaagcgctc gccctcgacc cgaaccgaga gaccctcgcc ctctccggaa
     2341 ccgatcgtga cccacatgcc gctccgcctc tcctacttgt aggcggcccg cccgcctccg
     2401 gggcgcggca gtttacgcgg ctgtcggcgt ctccgctgta gcggtttcgt ccgcttcagt
     2461 ctcgggtggc ttggcgcgcg gctccttggc ccactcctcg tcgaactcga cgcgccactt
     2521 gccgtcgtcg ccctgctcga gcttgagctt ggctcggaac gtgcgcccgc tgcggccccg
     2581 gaaacccgtg acctgcttct cggtcttgtt cgtggccatc agctccttga ccacgctcgc
     2641 ggggaggatc ttgcccgcct tcttcttcca gatcacgaag ccgcagccgg gatcctcgcg
     2701 cgaccagcac gagtagccct tgcggttctc gatcacgtcg cggccgcaga ccgggcacgg
     2761 gccgaggttg gcccgctcga tcttcacgtc cttcagcttg tcgagctcgg cgacggtctc
     2821 ggtggcgaac ttggcgatgt ccgacatgaa cgacggccgc gagtcctcgc cctgctcgat
     2881 cagcccgagc cggcgctccc agtcgccggt cagctcgggc gaggtgagcg ggtgggagcc
     2941 gagcaggcgg atcacctgga tgcccttctc ggtggccacc agcgcgcggc cgtcgcgctc
     3001 gatgtagcca acgtcgatca gccgctcgat gatcgccgcg cgcgtggccg gcgtgccgat
     3061 gccggactcc ttcatggcct cgcgcagctc ggcgtcctcg atgtccttgc cggccgtctc
     3121 catcgcgccg agcagcgacg cctccgtgaa gcgccgcggc ggctgcgtct ccttgcgcag
     3181 cgactccatc tcggtgacct tcaccgactc gccctggtcg agcttcggca ggagctggtc
     3241 gccgccggtg tcgtcgtcgg cgcgctcgcc ctcgacctcg gccccgtaca cggccttcca
     3301 gccggcctcg agcaggcgcc tgccgctcgt gcggaagacg tgctcggcaa cggtcgtctc
     3361 gacccgggtg cgctcgaaca cggcctccgg gtggaagacc gacaggaagc gcttggtgac
     3421 caggtcgtag atcttcagct cgtccgagcc catccgctcg aggtcgtgct cggacttggt
     3481 cgggatgagc gcgtggtggt cgtcgacctt gtcgtcgttg accacgcgcc cgagcgggag
     3541 cttgtcgagc gacacgacgt actccgcggc gcggcggtac tggtcgttgc ggccgacgag
     3601 ctcggcggtc ggcttgatct cctcgatctg gtcgctcggc aggtagcgcg agctggtgcg
     3661 cggataggtg atcgccttgt gctcctcgta aaggcgctgc gcggcggcga gcgtgcgccg
     3721 tgccgagaag ccgaacaggg tgttgccgtg gcgctgtagc gaggtcaggt cgtagaggag
     3781 ctgcgggcgc tcgcgctcct ccttcttctc gagcttcgtg atctcgccgg tgccctcgcg
     3841 ggcctcggcg acgatgcgcg ccgccagctc ctcgtcgatc cgcttgccgc ccaggtagcg
     3901 gccgcggtag gcgcggtcgg ggtcggcgag gaacgcggcc tcgacgagcc agtacggctc
     3961 gggcttgaac gcgcggatct cctcctcgcg gcgggcgacg agcgcgaggg tgggcgtctg
     4021 cacgcgcccc agcgacacgg cgccgtcgaa cgcggcccgc agccggatcg aggcggcgcg
     4081 ggtcgcgttc atccccacga cccagtcggc ctccgagcgc gagcgcgcgg cgtcctcgag
     4141 gctgtgcatc tcctcgccgg ggcgcaggcg ggcgaaggcc tcctcgatgg ccttgcgggt
     4201 catcgaggac agccagagcc gctgcacggg cttctcgatg ccggacgtct cgtaggtgta
     4261 ggcgaagatc agctcaccct cgcggcccgc gtcgcaggcg ttgacgatcc ggtcgacgtc
     4321 ctcgcgcgcg atgagcccgt gtaccgcctt gagctgcttg cgcgagcgct cgtcgttggg
     4381 cacgagcttg aacttgtcgg gcaggatcgg caggtcggcg aagcgccact tcttcagctt
     4441 cgggtcgtac tcgtcgggcg cggcgagacc aacgaggtgg ccgaccgccc acgtgatcac
     4501 gtagtcgtcg ccctcgatgt aggtcttgtc cttgctgttc ttgaaggagc cgggcagcgc
     4561 ggccgcgagg tcgcggccca cggacggctt ctctgcgatg acgagcgtct tccccatggc
     4621 tagctcggga cggtagcggt tggtgtcgac gcggccaagc cgctcggggc cgctagggcc
     4681 gcggcatcgg cccgcggctg ccgagccgct tgccggaggg gagcgagacg atcgtcatcg
     4741 ccgcgttgtc gtcctcgtcg ttctcccaga tcccgttgcc cgggtcggcg acgtggatga
     4801 agccgtagcg gccggtcagg ccggtgatct cgatgtactg ctcgtggtag gtcgacggat
     4861 agacatctga ccagcccacc gaggtcccga gggtgaccgc gttcgcgtgg cggttctggt
     4921 tgcaggccgg gtacacgcgg ccgcgcggcg agcccgccat cggttgggtc cggacgaggt
     4981 cgcgcaggca gtagtactgc ttcgggccgg tgcggacccg ctcgacgggg ttgtcgttct
     5041 cgtcgagcgt ccagagctcg aagcgcccgg cgtccttgta cttccagtag ccgccctggc
     5101 ccgggatgtt cttgaagccg aggcgcgcgg gcgtctcgac ggtgcgccgg cctccgccgc
     5161 ggagcaggat ccgctggttg gcctccatgt agtgcttgcc gtatcgcttg ccgcgcagct
     5221 cggcgggacc ctcgccgcgg ctgtcgatcg agttggtggc gtgcagtagc cgcctgccac
     5281 tgcgcgtctt cgagaccatc aggtccgagg gcggctgcat gcgcaggttc gggcagagca
     5341 gctcggccgc gtcgaggccg aggcacgggt tgtcgatcac gcccgcgccg ggtacggggg
     5401 tgccgccctg accctgggcc gcggtggcca gacccagcgc gagcacgcac gcggacagga
     5461 gtgcgagggg ggtacgcacg atcgaccggt atagcaccgg cgtgtaagca tcgatgcgct
     5521 ccgcttcccg cccccaccca ctcggctttc ctgacttatg ggtccatggc gcccataacc
     5581 caggaaagtc gaacgaaagg ccgcgtgctc agtgcgtgca ggggccggtg tccacccgct
     5641 cgtttgcgcg ggccagcccc tcgcggacga tcgagtccgg cactccgtag ccgccgcgcg
     5701 ggccgccgcc ggtggtcgag gcgaagaccg tcgccagcac gcggccgcgc gcgtcgacca
     5761 gcggaccgcc cgagttgccg gagcggacca cgccgcgcag cgccgtcacg cgtcggcgca
     5821 ccggaccgcg gccataggag tcctgggaga gcaccgtcgt ggtggcgccc aggcgcgcgg
     5881 cgcggatgct gagcggcccg ttgcccggat agccgaccac ggcggccgag gcgcccacct
     5941 cggcgtcctc gttcagggtg agcgccggag tgcccgcgag cccggtcgag cgcaggatcg
     6001 ccaggtcgtt gcgcgagtcg taccagatgg cgtcggcgtc gtggcgcgga ccctcaccgc
     6061 cgagctggac ggtcgtgtcg tcctggccgg cgaccacgtg cgcgttcgtc acgacgatcc
     6121 cgtcgccggc cacccagccc gagccctcga cgccgagccc gcatgccgtc ccgaggatct
     6181 tcactgtgct ccgcgcggcg cggcggatgt ccgggtcgcg cgcgatgcgg gaggtcggcg
     6241 gagccacctc cggggccggg cccctgaagg tcggggacgg gtcgaagcgc gcgagggtgt
     6301 tcagcagcga gcgcggcggg aacgcgtcgt tgagccgcgt gaggatggcc gagcgctgta
     6361 tgtcgcgccg gaactcgcgc gcgccgggcg tctgaagcgc gatcgccccg agcatccacg
     6421 cgagcccgag ccccgcgcag gcgagcagca gcgcgccgcc gacgccgtcg gcgacgatcc
     6481 cgagcccccc gcgcacgcgc cgacggagct ggaatcccac cgtctccagc acggaggcga
     6541 gcacgccgcc gatcaggaac gcgccgacga gcgcgaacac cggcgcatac ggcgagtgcg
     6601 acccgtcgtc cagcagcagc gggcccagtc gcgacccgac gaagccgccg ccgacgaaac
     6661 cggcgagcga aagagcgcca acaagcagcc cctgcgcgta accccatacg gccatgagca
     6721 ccgtaaaggc cacgatgatc caatcgattg cggtcatgcg cgggtgcgcg ggcggcgccg
     6781 gccgccgggc ggacggtcac actagccgct gacggtggcg ttccaggacc tatcagcgga
     6841 gcgccggcgg cgtctgaggc gccggaccgt gccgctcctg gccgccgccg cgacggtgat
     6901 gctgctcctc gcggtgctga acgcgagctg cggagcctcc gaggaggagg tcaccgccgg
     6961 gcgcttcgcg accgcgtggg agcgcggcga ctgggaggcc atgcaccgcc agctcacgcc
     7021 ggccgcgcag cgccgcttcc cgcccgatgc gctgcggcgt gcttacgagg cggccgccgc
     7081 caccgcgacc tccaccgccg tcaccaccgg agagccgcgc ggcgacggcg acggcgccgt
     7141 ggtcgaggtc accgcgcgca cgcgcgtgtt cggcccggtc cgcggtgagc tgcgactgcc
     7201 ggtggaggac ggccgcgtgg actgggcgcc gcacctcgcc ttcccggggt tgaaggaggg
     7261 cgagctgctg acccgccgca gcgagccgcc gcgccgcgcg ccgatcctcg cccgcgacgg
     7321 cacgcggatc gtcaccggcc caggggagca gcgcgcgatc gtcttcgtcc cggctgcggg
     7381 catcgccggc gaggtcgccg ccgcggcgcc cggccccgag cgcgaggccg tctacgcgcg
     7441 cgggttcccg cgcacatggc cgatcggccg caacgggctc gagcgggcgg tcgaggaccg
     7501 ggtcgccggg cgcccgggtg ggacgctgct cgccggcccg cgtccgatcg cccgcgccgc
     7561 cccccgcccc gccgccgccg tgcgcaccac gatcgacccc ggtgtgcagg ccgccgccgt
     7621 ccaggcactc gccgggcgcc tcggcgggat cgtcgcgctc gacgcacgcc gcgcggaggt
     7681 gcgcgcgctc gcgggcctcg cctactccgc cccgcagccg cccggctcca ccttcaagat
     7741 cgtgaccgcc tccgccgccc tagaggcccg gcgggtgaag gcgaagacga ggttcccggt
     7801 ctcgacccgc gccgtgatcg agggcgtcga cctcgagaac gcgaacggcg agtcctgcgg
     7861 cgggaccttc gagaactcgt tcgtccactc gtgcaactcc gtgttcgcgc cgctcggcgt
     7921 gcgcgtcggc gccggcccgc tcgtggcgat ggccgagcgc ttcggcttca accgaccgcc
     7981 gcccctggcc ggcgcgacgc agagcacgct cccgcaggcc gaggcgatcg ggtccgacct
     8041 cgacgtcggg tcgacggcga tcggccaggg caaggtgctc gccacgccgc tcacgctcgc
     8101 gcaggtcgcc cagacgatcg ccggcggcgg cgtgcagcac acgccgacgg tcgtccgcgg
     8161 ggcgcccgcc gcgcgcccgc ggcgcgtggt ctcggcgcgt acggcgcgga cggtgcgccg
     8221 cttcatggtc ggggtggtgc ggagtggcac cggtacggcg gcggcgatcc cggggacggt
     8281 cgtcgccggc aagaccggga ccgccgagct cggcacgacc cagggcgaca ccggcgccga
     8341 cgccggctcg accgacaccg acgcctggtt cgctgccttc gcccccgcgc gcaagccgcg
     8401 gctcgccgtc gcggtgatgc tcgtcagggc gggggcgggc ggccagaccg cggcgcccgc
     8461 cgcgcgggtg gtgcttcagg ccgggctcga ctaggagcgc cggggcctcc tacaggtcga
     8521 gctcgatgat gcccttctcg accttgccct cgggggtggt ctcctcgatc tcgagctcca
     8581 gcggccggtt ctcgagcgtc tggacgggga actcgaagat gaggagcgcg ccggcggtcg
     8641 gaccggtggc cgccgggctg ccggcctccg gcacgcaggc gtgcttgctg agccgccggg
     8701 cgcggtaggc gaagacgttg tccggctcga cctcgagggg ctcgaactcg ttgccctgcg
     8761 agtccttcac gacgaagtgc tcgaccgggc gcttgaagcc ggcgctctcg ttgcagacgc
     8821 gcatgaacac gccgtagtac gtgaagccgg gcttcggctc ggggccgttg tagaagccgc
     8881 ggtcggaggc gaggtgctgg ttgatctgcc gggtgatgta gaccgtgtac ttcagcccgg
     8941 ccacctcggt gccgagtccc tcgcgcctcg gctcgtcctg cggggcctcg cgctggcagc
     9001 ccgacgcggc ggtcgcgagg gccaccaaaa gacaggccag gagggcgctc cggaggctgg
     9061 gaaggcgcat cggcggcgga actctatccg ctcgacgcgc ggcgcccgga cccggtgtgg
     9121 acgtccggct ggtccgccaa ctcgactccg aagcgcggcg ccttctgcgg ctcccagccg
     9181 gccaccgtgg ctcgcgccag cggctcctcg cgctgctcct cgaagaccgc ccgcagccgc
     9241 tggagcgcga ccttcgcctg ccgcttcgcc tggctcttgg ccccgaaccg cgcgaagaac
     9301 cgctcgcgaa cggtgcccgg ctcggtccag accgtcatct cgacccgcgt cagggtgcgg
     9361 ccctggcgcg agaactcgaa cacgatctcc cccagggtca ggttgagccg cccgccgcgc
     9421 gtggcctcga cgatccgccg cggccggtcc gcctccacga tgtgcgtctc ggcccactgg
     9481 cggttgcgct ggctcagccg gtagcgcgcc gccgcgccca ccccgctcga gcgcgggtgt
     9541 gtcaggcgca gctccgactg ccagtggtcc atccacgcgg tccggttcgc caggtcggcg
     9601 acgaagtcga agatctcctc gcgcggggcg gcgatcgcgg tgtgaacggt gagcgggacc
     9661 atgtgcggcg tgatcgtatg caatggcgcg cccgcccgga ggtatccggc ccatcccgca
     9721 gaatgtgtgg gccatgccgc gcgacatcgc atcgatcctg ctgcgcaaca gcctcggcgc
     9781 ccttcacacg gaccgcccct cctgctcgcg ctgcaggcgc ctgccgctca ccggcgagct
     9841 gatgcacgag ctcgagtccg gccgcgaggt ctgctcgctg tgcctctcgc agtcggacgg
     9901 ccgcgacggc gagccggtcc gctccgagcg tgtacgcgcc acggacaagc cgctgtcggt
     9961 cgttccccgc gccgcctgag cgaccgcgcc cgaacgcgct ccgcgccgcc tgagcggcca
    10021 cgcctgaacg cgctccgcgc cgcccgcggg accccgcaaa ttgcgctgaa aagcctatgc
    10081 gatgggggct atccccgata tggggccctt gtggggttcg ttatcatccg cattatcttc
    10141 ggaggagaga ccgatacggt ccacgccggt acgctcgccg gcgcagggtg cttcagatgt
    10201 gactcgtgcg gctatgcagt cgccctgcac gagctcgacg aggtgcccgc ctgcccgcat
    10261 tgcgaggggc gcgatttccg gcgctcgtcg atcttcgcgg agctgtccct ggccgagccg
    10321 aacggctcga tggacgaccg gccggcctgg ctcgccgagg cgcgcgaggc gctcgtcacg
    10381 aggggcgact acatcgccta cgacgacggc gaccgggtcc gcgtggtcgc gctccaggac
    10441 gggtggacgc ggatcggccg cagtctcgcg gcgcacgtgc gcttcgacga cccgaccgtc
    10501 tcccgccgcc acgcactgct tcaccgccag gacgagggcg cccgcatcct cgacgaccgc
    10561 agcctgaacg gcgtgttcgt caatggcgcg cgcgtggaca tgcgcgagct cgaggacggc
    10621 gaccagatct cgatcggacg cttcagcctg catttcatgc gcctcggcgc cggcgccaag
    10681 ccggcggcga acgaggcgca gcccgatcgc gaccaggtcg cggtcggcta agtccgcagg
    10741 ttcgagggag ggagtcgaac gggcccgcca tgcgggcccg ttcgcgtttc cggggccgcg
    10801 cgcacccgcg ctcgccgccg cttccggcag ccgcccgctc gcgcacccgc cgcccggcca
    10861 cacgcgcacc cgccgcccgc ttgggcgggc gcgtatattc ggccttcgat ggccacgacg
    10921 atcgcggtgc tctcacagaa gggcggaacg gggaagacga ccaccacccg caccctcacg
    10981 gacgtgctcg gccggatcgg gctcgacgtg ctggcggtgg acctcgaccc gcagggcaac
    11041 ctgtccgact acttcgacgt cccggccgac gccgacccga cgatcgccga cgtgctgggc
    11101 ggcaaggtca aggccgccga cgccgtccac aacggcgtga tccccgccaa cctcggactg
    11161 gccgaggccg agctgatcct cagcggcaag atgggccgcg agatgacgct gcgccgcgcg
    11221 ctgcgcgacc tcggccgccg ccacgacgtg atcctggtcg actgcccgcc ggcgctcggg
    11281 ctcctcacga tcaacgccgt cgtggccgcg gactacgccc tgatctccac cgaggcgcag
    11341 tacttctcgc tccagggcgt cgagcaggct ctcgagatca tcgagctcgc caaggagaac
    11401 ctgcacccgg acctcgagtg gctcggggtg gtgctgaaca tcgccgacat gcgcaccgtc
    11461 cactcgcgcg aggcgctcgc ccagctcaag gagcgcttcg gcgagaagct cttcgacgcg
    11521 gtcatccgcc agtcgatccg ctacgccgag tcggccgagc gcggcgtgtc gatcatcgac
    11581 taccgccccg agctgggcga cgactacctc gcgctcgccg gcgaggtgct cgcccgcatc
    11641 ggcatgaccg aggagagcgg gcgggtggcc gagctgcggg ccgagctggt cccggcttag
    11701 ccgcgccccg accccccgct cgggggaaat tccaccgccc gcgttcgctg actcgcccgg
    11761 tggggaacct ggcgggcatc aggcgcggcc gtcgcgcccg gctcgaacag ggggaagaca
    11821 tgaagaggtg ggcccttgcg gtttcggtgg gagcgatcgt gggcgcgttc ggtgctgtcg
    11881 acggcgccgc ggcggcgtgc tcgacggacg ctcgggacag ggccgcgagc ccgtccggga
    11941 cgaccgtggg cgccgccggt cagagcgcgt gggtcaatcc ctcgacctcg gccggcgagg
    12001 tgggcacgtc gaccagcgca ggatcgaacg agggcggtgg cgggctcagc ggcggcagcg
    12061 cctactactc cggcggcgac gcggtgaccg gcagctacac agagggcagc ggctccggca
    12121 gcgccagcac cctctcgggc ggagcctcga gcgacggtgc cgcgggcggc atctcgagca
    12181 gcggcgacct gagcggctcg ccggccgggc ttgcggtcga tgacgtgcag gccggcgcgg
    12241 tctgcgcgac gccctagtcg cgagtcagag caggggcccg ggcggcatga acgcccgggc
    12301 ccaccggtac gcgagcccgc ggagtcctag gatcgcggca tgcacgacgg gacgccgccg
    12361 agcctgctcg cggggctgcg ggtcgtcgag ctctcgcggt tgatcgccgc gcccctctgc
    12421 ggcctgacgc tcgccgacct cggggccgac atcgtgaagg tcgagtcccc gcgcggcgac
    12481 gacacgcgaa cgttccctcc gctcctcgcc gggggagaga gcgcgtactt tcaagccctg
    12541 aaccgcggca agcgcggcgt cgtgctcgac ctggacgacg gcgttcgccg ccgcgaacgg
    12601 cgagcacgtc caggtcgtcg cgctcaacga gcggcactgg cacgcgctct gcgaagcgct
    12661 cgaacgaccg gaatggctgg acgacccgcg ctgcgccgac aacgaggcgc ggttggccaa
    12721 ccgcgacttc gggaacgagc ggatcggcgc ggcgatgacg acgcgcacgg cggcggagtg
    12781 ggtcgagcga atcacccgcg ccggtggcct gtgcgagcgg gtgcgcgagg tcgaggaggc
    12841 gtgggcggac ccgctgctcg cggagcgcgg cctggtcggg cagacatcgg acggcgccgc
    12901 gctgccgctg gtctcgctcg cgcgggcggc cagcccgcgc gcgctggccg ccgcgcccgg
    12961 gctgggcccg cacagcgagg aggtcagggc ggagctgtct ggcggctagc cgcggcgctc
    13021 agcgcggcac ctgcgtcagt tcaccgcgtc gcactgcacg ttctgcgtgt tcacgccgac
    13081 gaagcagcgc tggttcgccg agacccacgt gacgatgtag aagggctccg tgtcgccggg
    13141 gtagaggggc cagcgcagcg tgatgtagtc cgggttgccg ctgccacagc ccttctgctc
    13201 gcagagcgag agcgcctcct ggaggtcgag cgtgatcggc agggggatgg cgttgtcgtc
    13261 gccccagggc gagtcgatgt actgcggggg cgtctggaac tgaccctcga agttcttgag
    13321 gatcaccgtg ctggccgggg acgtggcggg gtcgccgaag acgaactgcc acggggcccc
    13381 gaggagggtg aggttgatgg aggcctcgaa gagctccgcc tgcgggtagt cggccgtgac
    13441 gatcgagttc gcctcgtcga gcatctccag gaagttctgc ggtagctctg ccatgttctc
    13501 ctccaggtcg cgtgagtcgg ggcgggcgac acgttaactg gagatggaca agcttggttg
    13561 attggtatcg aagacctgtc cggagggagt ggcgatgcgt gaggacgtga ggatcggctg
    13621 ctgggcgggc ttctggggcg acacgcgcga ggccgcggcg cggatcgtgc gcggcgctcc
    13681 gatcgactac ctggtcgccg accacctcgc cgagatcacg atggcgctgc tcgcgcgtgc
    13741 tcgcgcgcgg ggtccggccg cgggctacgt ggcggacgcg gtcgaggcgc tcgcgccact
    13801 gctcggcgag atccacgagc gctcgatccg ggtcgttacc aacgcaggcg ggctcgatcc
    13861 gcgcggctgc gtggacgcgc tgcgcgcggc cgcagccgcg gcgggcgttt cgatgcgcgt
    13921 cgcgtgcgtg acgggcgacg acgtgctgcc gctggcgggc acgctgcgcg agcgcggcgc
    13981 ggagctcccg gagcagccgc tgacgatgaa cgcctacctc ggcgcgcggc cgatcgcgac
    14041 ggcgctcgac atgggcgcgg acgtggtggt caccggccgc tgcgcggact cggcggtggt
    14101 gctcggcccg ctcctccacg agttcggctg gggcgacggc gaccacgacc tgctctcggc
    14161 gggcagcctc gtgggccaca tcgtcgagtg cgggccgcag tgcctcggag ggctgttcac
    14221 cgactgggag gaggtcccgg ggtgggacga catgggccac cccatcgccg agtgccggcc
    14281 cgacgggagc gcgacgatca caaagccgcc cgggaccggc ggcatggtca cgccggcgac
    14341 cgtggccgag cagatcctct acgagatcgg ggatccggcc gcatacgtga tgccggacgt
    14401 cgtctgcgac tggcgcgacg tgcggctcga gcaggagggg cacgatcgcg tacgcgtcac
    14461 gggcgcccgc gggcgggcgg cgacggcgac ctataaggcg acagcgaccc ggctcgacgg
    14521 gttccggctc gtcgcgagcg cgatgttcgc gggcggggac gccgccggtc gcgcgcgccg
    14581 cgtgggcgag gcgatcctcg tgcgcgccgg ccggatcgcc gcggaggccg ggcacggccc
    14641 gttcaccgag acctcggtgg aggtggtggg agccggcgag gcggtcgtga agatcgcggc
    14701 ccggcatccc gagcgcgcgg cactggaggt cctcgcgcgc gagatcgcgc cgatggggct
    14761 cgtggcgcag ggcatgaccg gcctgttcgc cgggcgcccg cggcccgcgc cggtcgtgag
    14821 cctgacgcat gtgctcgtcc cgaaggcgga cgtggcggtg acggtcgagc tcgacggcga
    14881 gacggtcgcg gtccggcccg cggccacacc ggagggcgcc gcggccaagc cggagcgcat
    14941 cgcgcccgat cgcgccgcgc cggcccgcgc cgcgccggag cccgcgccgc tacccgagcc
    15001 cccgccgccg gccgtcgccg acccggtcgt cgtgccgctg cgcacgatcg cctacgcccg
    15061 cagcggagac aagggcaacg acgcgaacat cggcgtgatc gcccgccggc ccgagctcgc
    15121 cgactggatc cgcgaccagg tcacggccgc gcgggtagcg gaggtattcg gacgctggct
    15181 cgacggcccg gtgcgccgct acgcgctgcc cggcctggac gcgatcaaca tcgtgctcag
    15241 cgacgtgctc ggcggctccg gcggcacatc gtcgctgcgc ttcgacccgc agggcaagac
    15301 ctacgcggcg atcctgctcg acatgccggt cgaggtgccc gccgatctcc ttgcgcgcag
    15361 cagcggcgag tgagagggtg agcccggtgg aggcttccga cgcgcccgac cgctcgtcga
    15421 tcgggctgtg cttctccggc ggcggcttcc gggcctcgtt ctacgcgctc ggcatcatgc
    15481 gctacctcgc cgaggcgagg ctgctcgacc gcgtgagcgc gatctcggcc atctcgggcg
    15541 gctcgatcgc cgctgccgcc gccgccgaca ggtggaccga gttccagacg gccggcggcg
    15601 gcgtcgaggc attcctcgag aagatcgacg ggccgttccg gagcaccgtc acctccaaga
    15661 acatcacccg gcgctggctg atcgcctccc tcgccggcgt gatccccggc accgggggcc
    15721 gcggcggggc gtacgcgcga acgctcgccc acaacctgta cgagcacgac cgcgtcgccg
    15781 acctgccgag agagccggag ttcatcttca cctcgacgga catgtcgaag ggccgcgcgt
    15841 tccggatagc gccgggattc gtcgggagct gggactacga ctacgtcgag cccgcgccgc
    15901 ggtcgatctc gctcggcacg gcggtcgcgg cctccgcggc cttccccccg tcgctgaccg
    15961 tggtcgatgt gcggaccaag ggccttggct tccccaagcc cgctccgccc gtcctgtcgc
    16021 tggtcgacgg cggcgtgtac gacaacctcg gcctcgagtg gttccagggc gaggggtcgc
    16081 ggcgcccggc gtcggcgaag gagaagccgc cgttcacgat cgtcgccaac gcctcggggc
    16141 tgttcgccgt gaagaacaag cggttctggt cgctcacctc gctcgggcgc gacctgtcga
    16201 tccagtacga gcagtcgctg agcgtgcgca tccggtggtg ggtcgagacg cttcaggagg
    16261 gcgagcccgg cgtctacgtc gcgaccaaga ccgacccccg caaggagccg catctcgacc
    16321 ccgccgtggt cagggcggcc ctgccgagcg agctggtcct gccgctcgcg cggctgcgca
    16381 cggacctcga ccggttctcc accgaggagg ccgacctgct ctcctaccac gcgtactgga
    16441 ccgtgcacgc gcgcctgcgc acgtacgccg ccgacctcgc gctcgccgag ccggcgtgga
    16501 ccgactacgc ggacctctcg gccgccgaga ccgagcgcct gcgcaagctc ctcgagctcg
    16561 gctcccaccg cttcttccgc cgcgtgcggg cggcgctcag gcgctagccg ggcaccaggg
    16621 ctacagcgcc cgcacgtccg cgccgcccgg cacgaactgc gcgcgtctga cctcgcagtc
    16681 aggcgccgcc gtgcgcacgg cgtcgagcac cgtgccggtc gcctgccagt ggcaccagaa
    16741 gagcaccgcg gggccggcgc ccgacaccgt cgcgcccacc gcgcccagct cggcggcgcg
    16801 gcccaccagc tccatcgtcc gcgggtagag cgcggcgcgg cgcggctggt ggacccggtc
    16861 cgagagcccc cgccccacct gcgacaggtc cccgcgcgtg aggccgagga tcagtagaga
    16921 cgcgtgcgcg atgttgtgca ccgcgtcggc caccggcacc tcgtccggga gcgcggcgcg
    16981 cgcatcctcg gtcgggaccg ggtccggcgg gatcgcgagc accccctcga gccccggggc
    17041 cgggtcgaag cgcgcgggag tgtcccccgc gcagatcacg aagccgccca tcagcgcggc
    17101 cgcgacgttg tccggatggc cctccagcgc agcggccagc tcgaaaagcg gcgcgtccag
    17161 ctcgtacatc gagtccgccg ccacgagtcc ggccacgatc gcggcggcgc tcgacccgag
    17221 ccccgcgccg agcgggatct ccgagcggat gcggaaggtc agcccgccga cgtcgtcccg
    17281 aagcgcctgg aacgcccgca cgcagaggtt ctcgggcccc agcggcacgc ccggcacgtc
    17341 gcactcgacc gccagctcgc cgcccgtctc ctccacctcc agctcgagat agatcccgag
    17401 ggcggtcgcc agcgcgtcgt agcccggccc gaggttggcg ctcgacgcgg gcacgcgcac
    17461 gagccgccgc cgcttcacga ccccagcacc gcccgctcga cgtcgtcgat ccgcggctcg
    17521 cacggcacca ccggcgtcgc acgcgccagc gcggtcgtcg ggtccttcag cccgtgcccg
    17581 gtcagcacgc atacgaccct gccctccgcg ccgtacttca ggagccccgc caccgacgcc
    17641 gccgaggccg gctcgcagaa cacgccctcc cgctctccca gcagcgcgta ggccgcgagg
    17701 atctcgtcgt cggagaccgc tcgcacctcc ccgcgcgagg aggtgaacgc gtccatcgcc
    17761 tcctcccagc gcgccgggtt gccgatcctg atcgccgagg cgacggtctc cgggttctcg
    17821 accggcgcgc cgttcacgag cggcgccgcc ccctcggcct ggtagccatg cagccgcggc
    17881 gccaccccgt actcgccgaa gcccttccac caggcggtca cgttgccggc gttgccgacc
    17941 gggatgcaga gcacgtccgg cgcctcgccg agctggtcgc agacctcgaa cgcgcctgtc
    18001 ttctggccct cgatccggta cgggttgacc gagttgacca gcgcgatcgg gttgcggtcc
    18061 accagctcgc gcaccagccg cagcgcctcg tcgaagttgc cgcgcagcgc cacgacgcgc
    18121 gcgccgtgca tgagcgcctg agccagcttg ccggtcgcga tcttgccctc gggcacgatg
    18181 accgcgccgc gcaggcccgc ccgcgccgcg
//
LOCUS       DASVPL010000323         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_349980, whole genome shotgun sequence.
ACCESSION   DASVPL010000323 DASVPL010000000
VERSION     DASVPL010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349980"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VF517_18595"
     CDS             <1..191
                     /locus_tag="VF517_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8054998.1"
                     /translation="RRIGLSATGKIDSATWTALLSYSLESGRRAKAAAAPAVRVTPRS
                     ARLPARRYEIPPPAERAP"
     gene            complement(188..658)
                     /locus_tag="VF517_18600"
     CDS             complement(188..658)
                     /locus_tag="VF517_18600"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8054999.1"
                     /translation="MKGFPPDHAIRAARGDDFDGVTALLEGLGRPTVTDDTRDDARAV
                     YRAQVLDPNTHHMVAVAPGGDLVGFCSLHYRTRLNHASEEAWVPDLFVAEHARYRGIG
                     RGLLEEAERRAIARGCWHLTLESGHQRAEAHALYRGFRMRDSGRQFSKLLREGG"
     gene            714..1583
                     /locus_tag="VF517_18605"
     CDS             714..1583
                     /locus_tag="VF517_18605"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX8055000.1"
                     /translation="MAQIDWTDRPRLRSPLVVSAFKGWNDAGEAATAALGFLVESFDA
                     TEVARIDPEEFYDFTAVRPTVRLSEGRTRVVEWPENTLHAAHVSGAEHDLVLVQGVEP
                     SLRWRHFTGLVVEAARELGAHMVITLGALLADVPHTRPVPMTGIASDDALVGRLGYER
                     STYEGPTGIVGVLHHECARAGLSSVSLWASVPHYVAAAPNPKAALALIRSFEGVAGVA
                     VEARDLEESAEDYERQVSAAVASDPEVKSFVERLEQTLDEAESELPPDKIPSADAIAR
                     DFQRFLRQRGPGA"
     gene            complement(1609..1818)
                     /locus_tag="VF517_18610"
     CDS             complement(1609..1818)
                     /locus_tag="VF517_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8055001.1"
                     /translation="MDDWRRTARTDGSQRLFDDAAAARRFQRRLREVAPSDGHGRPAL
                     HYDESGYPIEDRASLAARVRRLITG"
BASE COUNT          244 a          722 c          730 g          259 t
ORIGIN      
        1 agcgccggat cggcctgagc gcgaccggca agatcgactc cgcgacctgg acggcgctcc
       61 tcagctactc gctcgagagc ggcaggcgcg ccaaggccgc cgcggcgccg gcggtacgcg
      121 tcacgccgcg cagcgcgcgg ctcccggcgc ggcgctacga gatccccccg ccggccgagc
      181 gcgcgcccta gccgccctcg cgcagcagct tcgagaactg ccgaccggag tcgcgcatcc
      241 ggaagccgcg gtagagggcg tgcgcctcgg cgcgctggtg gcccgactcg agggtcaggt
      301 gccagcagcc gcgcgcgatc gcccgccgct cggcctcctc gagcagcccg cgcccgatgc
      361 cgcggtaccg cgcgtgctcg gccacgaaca ggtccggcac ccaggcctcc tcgctcgcgt
      421 ggttgaggcg cgtgcggtag tgcagcgagc agaagcccac gaggtcgccc ccgggagcga
      481 cggcgaccat gtggtgggtg ttggggtcga gcacctgggc gcggtagacc gcgcgggcgt
      541 cgtcgcgcgt gtcgtcggtc accgtgggcc ggccgagtcc ctccagcagg gcggtcacgc
      601 cgtcgaagtc gtcgccgcgc gcggcgcgga tcgcgtgatc gggcgggaac cccttcatcg
      661 gccggacatt gtgaccctgt tggcgcgggg cgtcgttcgg gtaccgtcct ccaatggctc
      721 aaatcgactg gacggaccgg ccgcgcctgc gaagcccgct ggtcgtgtcg gcgttcaagg
      781 gatggaacga cgcgggcgag gcggcgaccg ccgcgctcgg cttcctcgtc gagagcttcg
      841 acgccaccga ggtggctcgg atcgaccccg aggagttcta cgacttcacg gccgtgcggc
      901 ccaccgtgcg cctgagcgag ggccgtacgc gcgtggtcga gtggcccgag aacacgctgc
      961 acgcggcgca cgtgagcggc gctgagcacg acctcgtgct cgtgcagggg gtcgagccgt
     1021 cgctgcgctg gcggcacttc acgggcctgg tcgtcgaggc ggcgcgcgag ctgggcgccc
     1081 acatggtgat cacgctcggc gcgctgctcg ccgacgtccc gcacacgcga ccggtgccga
     1141 tgaccgggat cgcgtcggac gacgcgctgg tggggcgcct cggctacgag cgctcgacct
     1201 acgaggggcc gaccgggatc gtgggcgtgc tccaccacga gtgcgcgcgg gcggggctgt
     1261 cgtccgtctc gctgtgggcg tccgtcccgc actacgtcgc ggccgcgccg aacccgaagg
     1321 ccgcgctcgc gctgatccgc agcttcgagg gcgtggcggg cgtcgccgtc gaggcccgcg
     1381 acctggagga gtccgccgag gactacgagc ggcaggtcag cgccgccgtg gcgagcgacc
     1441 ccgaggtgaa gagcttcgtc gagcggctgg agcagaccct cgacgaggcg gagtccgagc
     1501 tcccgccgga caagatcccc tcggcggacg cgatcgcgcg ggacttccag cgcttcctgc
     1561 gccagcgcgg gcccggcgcg tagcgcgcgc caccgccttc gtacgcgctc agccggtgat
     1621 cagcctccgc acgcgcgccg ccaggctcgc gcggtcctcg atcgggtagc cgctctcgtc
     1681 gtagtggagc gccgggcggc cgtggccgtc gctcggcgca acctcccgca gccggcgctg
     1741 gaaccgccgc gcggcggcgg cgtcgtcgaa caggcgctgc gacccgtcgg tacgggcggt
     1801 ccgtcgccag tcgtccatct ctcctcctac tgctcggggc cggggctccc cctcgaccgc
     1861 gaagtttccc gcgcgccgcg cgcatgtcca ggccgagcgc ctcaacatcc cgcattttgc
     1921 gcggaaagct cccgcgctca aggtggatcg tgcag
//
LOCUS       DASVPL010000324         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_194694, whole genome shotgun sequence.
ACCESSION   DASVPL010000324 DASVPL010000000
VERSION     DASVPL010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194694"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VF517_18615"
     CDS             <1..659
                     /locus_tag="VF517_18615"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX8055002.1"
                     /translation="RPGDTGTGAKVLPARRATPPVRPRPTIPPTRAPAGRPGQSSILT
                     QPPGSRPAARRGARTWPAPRYIALIIAGIVILGLGGTVGLMQLTKDEEQQPATRPPVT
                     DLEDPTRAPDPPDDTPAIDPGSVTVAVLNATTTNGLAVRVGSRLEAAGFRTGNVTNAS
                     ERARTESVVMYKPGKTAEARTVARRLDIGSTAPIDAASQALVGDAQVVVVLGADKAQQ
                     "
     gene            662..1450
                     /locus_tag="VF517_18620"
     CDS             662..1450
                     /locus_tag="VF517_18620"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX8055003.1"
                     /translation="MPDFLELPSRPAKPRDSGLTHVIDTGLSTAEAQGLVASAGDYVD
                     LVRLGWGSAYVTHDLEAKLDAYRAGGVTVMLGGTLTELAWLHGRVDELRAWLGELGIE
                     HVEVSSGTVAIPEEEKFALIESFAGDFTVFAEVGEKDPAALLAPYRWVSLIRSALDAG
                     AAWVTCEGRATGDAGLYRPDGEPRTGLIDEIVHEVDAARLIFEAPQKDQQVWFIRQLG
                     GAVNLGNVLPADVLSVETLRTGLRADTLELFHGRASTADPPAGA"
     gene            1447..1992
                     /locus_tag="VF517_18625"
     CDS             1447..1992
                     /locus_tag="VF517_18625"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8055004.1"
                     /translation="MILLARHGETDDNAPPRRVQGRLDPPLNERGREQAHALAREVAD
                     GHEVAKLWVSNQRRARETAAIVGTAIGLEAEVDERFAEADWGRWQGQLVDDIERDEPE
                     LWAAFLSSRPDFRFPGGESLEEHIARTWAALDDVARGPLPALVVCHGGTIRCALARTH
                     PDGIAGIGPYRIANGALIALP"
     gene            2021..3778
                     /locus_tag="VF517_18630"
     CDS             2021..3778
                     /locus_tag="VF517_18630"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX8055005.1"
                     /translation="MSSSAVARVVFALLVAATGAAFFVTQRLKTQDPVVKRLALQRFF
                     SPNGDGRKEEAGISFQLPEGDRVTVEVVSGAGDRIRRLADDRPLSAGKHRLRWDGRDD
                     RGRVPRDGTYYVRVALREQARAVTGPRGIVLTTAAPQPRIVSVGPGRIAAASRVPVTV
                     RFVGPAVVRPVFRVWRTDGRVREVARFTVPRGQHVGSWDGRVNGRAAPEGSYAFSVTV
                     QNRALIEGTAPGRLPPRRDGARQGTGVRIAGPEAILPVEAVPAGTVARIPVVGASGAL
                     SWRFARVGSRRVLRRGRGRAGQVAFRVPARVPAGEYVVRIAAGSRPIRVPLTIRKPTR
                     ASVLVILPVITWQGRNAVDDDLDGFADTLDVSRSVPLTRPLAGGRLPSGFGDEVEPLL
                     GFLQRRGLRYDIDTDVGMVRQGGVDPKARSGIVLAGDERWLPAAAGRRLREYVEQGGT
                     VVSLGTDSLRREVDLDRDALADPTGPRRTTLFGEDTRPLESLAAPLVATQDELGLFSG
                     TGSVVGLFTRFEQAERPAAGGRLVTNAGRDPAKPAFAAYRLGRGLFLRVGTPQWSRAL
                     RSRPEVAAVMRRIWTQLER"
     gene            3778..>4506
                     /locus_tag="VF517_18635"
     CDS             3778..>4506
                     /locus_tag="VF517_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8055006.1"
                     /translation="MAPLATGTALKGSLAIAAALVVAGTFLLRAPRARALAAVVALAL
                     APVLVIAELWDSAQIHSLRDNPTRAAVAALAAAIAVFVLAAVLRRRPGLLPLLAVLAL
                     PIRIPLETGGGSANLLVPLYGVVAAGVLAYALDRLRGGGSWSERRPTAPELALLAFVA
                     LYAIQSLYSADFETAVKNIAFFYIPFALLLKLLTVVPWSPQLVTRCFAAAAAMAVLFV
                     LVGFYEYATRDLLWNSKVIESNQFE"
BASE COUNT          527 a         1710 c         1688 g          581 t
ORIGIN      
        1 cacgcccggg tgacacgggc accggcgcca aggtccttcc ggcgcgccgc gccacgccgc
       61 cggtgcgccc gcgtccgacc atccccccga cgcgcgctcc ggccggccgc cccggccagt
      121 cctcgatcct cacccagccg cccggctccc gcccggccgc ccgccgcggc gcccgcacct
      181 ggcccgcccc gcgctacatc gcgctgatca tcgcgggcat cgtgatcctc ggcctcggcg
      241 gcaccgtggg cctcatgcag ctcaccaagg acgaggagca gcagccggcc acgcgcccgc
      301 cggtcaccga cctcgaggac ccgacccgcg cgcccgaccc gcccgacgac acccccgcga
      361 tcgacccggg aagcgtcacc gttgcggtgc tgaacgcgac gacgaccaac ggactcgcgg
      421 tccgtgtcgg ctcgcggctc gaggcggccg gcttccggac cggcaacgtc accaacgcca
      481 gcgagcgcgc ccgcaccgag tcggtcgtga tgtacaagcc cggcaagacg gccgaggcgc
      541 gcacggtcgc gcgccggctc gacatcggct cgacagcgcc gatcgacgcg gcgtcgcagg
      601 cgctggtggg cgacgcgcag gtcgtcgtcg tgctcggtgc cgacaaggcc cagcagtagg
      661 ggtgcccgac ttcctcgagc tgccgtcgcg gcccgcgaag ccgcgcgaca gcggcctcac
      721 gcacgtgatc gacaccggcc tctcgaccgc cgaggcgcag ggactcgtgg cgagcgcggg
      781 cgactacgtg gacctggtcc ggctcggctg gggctccgcc tacgtgaccc acgacctcga
      841 ggccaagctc gacgcctacc gcgcgggcgg cgtgacggtg atgctcggcg gcacgctcac
      901 cgagctggcc tggctgcacg gccgcgtcga tgagctgcgg gcgtggctcg gcgagctggg
      961 catcgagcac gtggaggtgt cgagcggcac cgtcgcgata ccggaggagg agaagttcgc
     1021 gctgatcgag tcgttcgcag gcgacttcac ggtctttgcc gaggtggggg agaaggaccc
     1081 cgccgcgctg ctcgcgcctt accgctgggt gagcctgatc cgctccgcgc tcgacgccgg
     1141 cgcggcctgg gtcacgtgcg agggccgagc caccggcgac gcgggtctct accgccccga
     1201 cggcgagccg cgcacggggc tgatcgacga gatcgtccac gaggtggacg ccgcccggct
     1261 gatcttcgag gcgccgcaga aggaccagca ggtctggttc atccggcagc tcggcggcgc
     1321 ggtgaacctc ggcaacgtgc tccccgccga cgtgctgtcg gtcgagacgc tgcgcactgg
     1381 cctgcgcgcg gacacgctcg agctgttcca cggccgcgcg tccacggcgg acccgccggc
     1441 cggcgcgtga tcctgctcgc gcgccacggc gaaaccgacg acaacgcgcc gccgcggcgg
     1501 gtgcagggac gcctcgaccc gccgctgaac gaacgcgggc gtgagcaggc gcatgcgctc
     1561 gcgcgcgagg tggcggacgg gcacgaggtc gcgaagctgt gggtgagcaa ccagcgccgc
     1621 gcccgcgaga ccgccgcgat cgtgggcacg gccatcgggc tcgaggccga ggtggacgag
     1681 cgcttcgccg aggccgactg ggggcgctgg caggggcagc tggtcgatga catcgagcgc
     1741 gacgagcccg agctgtgggc ggcgttcctc tcctcgcggc cggacttccg ctttccgggc
     1801 ggcgagtcgc tcgaggagca catcgcccgc acctgggccg cgctcgacga cgtagcccgc
     1861 ggtccgctcc ccgccctcgt cgtctgtcac ggcggcacga tccgctgcgc cctcgcgcga
     1921 acgcaccccg acggcatcgc cggcatcggc ccgtacagga tcgccaacgg cgcgctgatc
     1981 gcgctcccgt gaccgcagcg cgcccgggta cgctccccgg gtgtcgagca gcgcagtcgc
     2041 ccgcgtggtc ttcgcgctgc tcgtggccgc caccggcgcg gccttcttcg tcacccagcg
     2101 gctgaagacg caggaccccg tcgtaaagcg cctggcgctc cagcggttct tctcgccgaa
     2161 cggcgacggg cgcaaggagg aggccgggat ctcgttccag cttccggagg gcgaccgggt
     2221 gacggtggag gtggtgagcg gagccggcga ccgcatccgc cggctcgccg acgaccgccc
     2281 gctgagcgcc ggcaagcacc ggctgaggtg ggacggccgc gacgatcgcg gacgcgtgcc
     2341 gcgcgacggc acctactacg tgcgcgtggc gctgcgcgag caggcccggg cggtcaccgg
     2401 gccgcgcggc atcgtgctca ccacggcggc cccgcagccg cggatcgtgt cggtcggtcc
     2461 gggccggatc gccgcggcga gccgcgtgcc ggtcaccgtg cgcttcgtcg ggccggcggt
     2521 cgtgcgcccg gtcttccgtg tctggcgcac ggacggccgc gtgcgcgagg tcgcgcgctt
     2581 cacggtgccg cgcgggcagc acgtgggaag ctgggacggg cgcgtgaacg ggcgcgcggc
     2641 gccggagggc tcgtacgcgt tctcggtcac cgtgcagaac cgggcgctga tcgagggaac
     2701 ggctcccggc cggctgccgc cgcggcgcga cggggcgcgc cagggcaccg gcgtgcgcat
     2761 cgcggggcca gaggcgatcc ttcccgtgga ggcggtgccc gcgggcacgg tggcgcggat
     2821 ccccgtggtc ggggcgagcg gcgcgctgtc gtggcgcttc gcgcgggtcg gctcgcggcg
     2881 ggtgctgcgc cgcgggcgcg gccgggctgg gcaggtcgcc ttccgcgttc ccgcgcgcgt
     2941 gccggcgggc gagtacgtgg tccgcatcgc cgccggctcg cgaccgatcc gcgtgccgct
     3001 cacgatccgc aagccgaccc gcgcgagcgt cctcgtgatc ctgccggtga tcacctggca
     3061 gggccgcaac gcggtcgacg acgacctcga cggcttcgcc gacacgctcg acgtctcgcg
     3121 ctccgtcccg ctcacgcggc cgctcgccgg cggccgcctg ccgtccgggt tcggggacga
     3181 ggtcgagccg ctgctcgggt tcctccagcg gcgcgggctg cgctacgaca tcgacaccga
     3241 cgtggggatg gtgcgccagg gcggcgtcga cccgaaggcg cgcagcggga tcgtcctggc
     3301 cggcgacgag cgctggctgc ccgcggcggc cgggcggcgg ctgcgcgagt acgtggagca
     3361 gggcggcacg gtcgtctcgc tcggcaccga ctcgctgcgc cgcgaggtgg acctcgaccg
     3421 cgacgccctc gccgacccca ccgggccgcg gcgcaccacg ctgttcggcg aggacacgcg
     3481 gccgctcgag agcctggccg cgccgctcgt cgcgacccag gacgagctgg ggctgttcag
     3541 cggaaccggc agcgtggtcg ggctgttcac gcgcttcgag caggcggagc ggcccgcggc
     3601 gggcgggcgg ctggtgacga acgcgggccg cgacccggcc aagccggcgt tcgcggcgta
     3661 ccggctcggc cgcgggctgt tcctgcgggt ggggacgccc cagtggagcc gcgcgctgcg
     3721 ctcgcgcccc gaggtggccg cggtgatgag gcggatatgg acgcagctgg agcggtaatg
     3781 gcccccctcg cgacgggcac cgccctcaag gggagcctcg ccatcgcggc cgcgctcgtc
     3841 gtcgcgggca cgttcctgct gcgcgccccc cgcgcccgcg cgctcgccgc cgtggtcgcc
     3901 ctcgccctcg cgccggtgct cgtgatcgcc gagctctggg actcggctca gattcacagc
     3961 ctgcgcgaca acccgactcg cgcggcggtc gcggccctcg cggcggccat cgcggtcttc
     4021 gtgctcgcgg ccgtcctgcg gcgccgtccc ggcctgctgc cgctgctcgc cgtgctcgcg
     4081 ctgccgatcc gcatcccgct cgagaccggc ggcggctcgg cgaacctgct ggtgccgctc
     4141 tacggggtcg tggcggccgg ggtgctcgcc tacgcgctcg accggctgcg cggcggcggc
     4201 tcctggagcg agcggaggcc gaccgcgccc gagctggcgc tgctcgcgtt cgtcgccctc
     4261 tacgcgatcc agtcgctcta ctcggccgac ttcgagaccg ccgtcaagaa catcgcgttc
     4321 ttctacatcc cgttcgcgct cctcctgaag ctgctcaccg ttgtcccgtg gtcgccgcag
     4381 cttgtcacgc gctgcttcgc cgcggccgcc gcgatggccg tgctgttcgt gctcgtcggc
     4441 ttctacgagt acgcgacccg cgacctgctc tggaacagca aggtgatcga gtcgaaccag
     4501 ttcgag
//
LOCUS       DASVPL010000325         5164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U3178
            k141_350727, whole genome shotgun sequence.
ACCESSION   DASVPL010000325 DASVPL010000000
VERSION     DASVPL010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959806
            Sequence Read Archive: SRR10946703
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:16:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,730
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350727"
                     /isolate="SMAG_U3178"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3843
                     /locus_tag="VF517_18640"
     CDS             <1..3843
                     /locus_tag="VF517_18640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HEX8055007.1"
                     /translation="GAAAERLRTHLAERPAPLGALAASLAGGRAALAERACLVASTPD
                     ELDGALGALAAGRPGPRTARGRAGPARARVAFLVPGQGAGAAGALAGLLDEEPVARTL
                     AALAEPLGPLERLPLAALLEPGPDSEAALARTEHAQPALYALALALGAWWAAHGVEPD
                     AVLGHSVGAYAAAALAGALDPQAGARLVAARGRLMGALPDGGAMAALAAPRERVERAL
                     GALGAPGLAIAAENAPEETVVAGPAAALEALCAQLGASGVRARRLAVSHAFHSPLVEP
                     ALAALAGELGRAGLRAPSGTLVSDHSGALAGAELATPGYWLEHARAPVRFAAALRTLG
                     ELGADCLVELGPGQTLLGLARRTLGAGPAALASLRPGEPGPLSLRRALAGAWCRGAAL
                     DWAAAAPPAGPALELPTYPFERRRHWLPEGGAPRAGGPGRGPAHGEGLRPEPVATPLA
                     AAIYASELCPARLGLLREHRVYGLVVVPGVAYLELILAAAERDGAERPAVSALELVAP
                     LVLGDEEARAVQVIVTDAGDGTRAAELHGQEPGGGWRLHARARVTPLSPDRPAEPVAL
                     ERLREHCGAALGGEEFYASIWHPDFELGDSFRLIEQVHRGETEALALLRVPDGATGVR
                     PELLVLDACVQTLVAPAGAHVDHQLPVALGTGHGRIEVHGPLGGERLWCWARLDGASG
                     AAGEIAGEVRILDEAGTPLAEVDGLRFRRVGRETLKRLAADPAADAPDARSTRHPAAP
                     DRAQLAAADGPARREALGRYLATVLGAVVGMSAEELDPAQPLTVLADSLMIAELKLRV
                     ETDFDVEVPIERFFDDAGLDDLAAWLADEALAEPAPEPARTNGHRRAPAAMTVEQLTA
                     AAELDPAIRPAAPADPRSAADPRAVLLTGATGFVGAFLLAELLERTTADVLCLVRADT
                     PAEGEERIRANLLRHCLDDHAAGRIVPVLGDLTQPRLGLSDSAFKGLAASADAVYHCG
                     AVVKWTYPYSALAPPNVDGTREMLRLATAARTLPFHFVSTVGVFSSLEYDRASVAEDE
                     PLERSGPLTVGYAQTKWVAERMATAARERGVPVSIHRPNVGAHSATGAFNPQDHICLA
                     LKGCIELGAAPESSVPVQMAPVDFVAAALVDRALACDHRTFHLVNPEPIRWTALFQLV
                     RELGYPLATMSFEDWAERVGRTAGSLGALASGPLQQAALPRFDCAQTLAALPPERRRC
                     PRIDLDLLRTYFARFAETGFVETPPRGGLIEGDRHKSPHPAVAAAGTDQGARGHPGPV
                     AGTAPANDRERGEEHGYS"
     gene            3830..4906
                     /locus_tag="VF517_18645"
     CDS             3830..4906
                     /locus_tag="VF517_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8055008.1"
                     /translation="MATASREERLEERIRDLEDELHRRSSGREGRGRRQSSGRWGEYE
                     DTREDFADFTDRKVDEISRLVTGSFRAGLEALRVAAESTDYFVDDVLERNLPNADEDS
                     TDVARRLPRDLGRGFSRSLERGLDIPGRASERFNRTYTYEEGGQPWHSRRGRRGGRGR
                     RGRGRRDEYTRRRAPSGEDYERWSQRELYDEAAERGIDWSDLTREELVEELRKDERGY
                     EDWPTPELYRRARDLEIRDRADMDRDELIDAIRRREERANPARSPLERRRGGSGDGES
                     TEEDYDKWSRSELEERAAACGIENASEKSREELVEAVKRHEPPPEDQSRTELYQRARE
                     LDIEGRSQMSREELVEAIKSHEKS"
     gene            4903..>5164
                     /locus_tag="VF517_18650"
     CDS             4903..>5164
                     /locus_tag="VF517_18650"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEX8055009.1"
                     /translation="MNYGDGGSGPGGVASATPWVVRFDPRPTAAVRLICLPHAGAGAS
                     MYLRWSRALPDWVEVVAVQLSGRETRRREPLRHAAGELVEDLA"
BASE COUNT          592 a         2037 c         1962 g          573 t
ORIGIN      
        1 ggcgccgcgg ccgagcgcct gcgcacccac ctggccgagc gcccggcgcc gctcggcgcg
       61 ctcgccgcga gcctggccgg cgggcgcgcc gcgctggccg agcgcgcctg cctggtggcg
      121 agcacgccgg acgagctcga cggcgccctc ggcgccctcg ccgccgggcg cccgggcccg
      181 cggaccgcgc gcgggcgcgc cggaccggcg cgcgcccggg tcgcctttct cgtgcccggc
      241 cagggagccg gggcggccgg ggcgctcgcc ggcctgctcg acgaggagcc ggtcgcccgc
      301 acgctcgcgg cgctggccga gccgctcggc ccgctcgagc gcctgccgct ggcggccctc
      361 ctcgagcccg gcccggactc cgaggcggca ctcgcgcgca ccgagcacgc ccagcccgcc
      421 ctctacgcgc tggcgctggc gctcggggcc tggtgggccg cccacggcgt cgagcccgac
      481 gccgtgctcg gccactcggt cggcgcctac gccgccgccg cgctggccgg cgcgctcgac
      541 ccgcaggccg gggcgcgcct cgtggccgcg cgcgggcggc tgatgggcgc cctgcccgac
      601 ggcggggcga tggccgcgct cgccgccccg cgcgagcggg tcgagcgcgc gctcggcgcg
      661 ctcggcgccc ccgggcttgc gatcgccgcc gagaacgccc ccgaggagac cgtcgtggcc
      721 ggcccggcgg cggcgctcga ggcgctctgc gcgcagctgg gcgcgagcgg cgtgcgcgcg
      781 cgccggctgg ccgtctcgca cgccttccac tcgccgctcg tcgagcccgc cctggcggcg
      841 ctcgccggcg agctcgggcg ggccggcctg cgcgccccgt cgggcacgct cgtctcagac
      901 cacagcggcg cgctcgccgg ggccgagctg gccacgcccg gctactggct cgagcacgcc
      961 cgcgccccgg tccgcttcgc cgccgcgctg cgcaccctgg gcgagctcgg cgccgactgc
     1021 ctggtcgagc tcggcccggg ccagacgctg ctcggcctgg cccggcgcac gctcggcgcc
     1081 ggccccgccg cgctggcctc gctgcgcccc ggcgagcccg gcccgctcag cctgcgccgc
     1141 gcgctcgccg gggcctggtg tcgcggcgcg gcgctcgact gggccgccgc cgccccgccg
     1201 gccgggcccg ccctcgagct gccgacctac ccgttcgagc gccgccgcca ctggctgccg
     1261 gagggcggcg cgccgcgcgc gggcggcccc ggccggggcc ccgcccacgg cgaggggctg
     1321 cgccccgagc ccgtggcgac cccgctggcg gcggcgatct acgccagcga gctctgcccg
     1381 gcgcgcctcg ggctcctgcg cgagcaccgc gtctacgggc tcgtggtggt gccgggcgtc
     1441 gcctacctcg agctgatcct ggccgccgcc gagcgcgacg gggccgagcg cccggcggtc
     1501 agcgcactcg agctcgtggc gccgctcgtg ctgggcgacg aggaggcgcg cgcggtgcag
     1561 gtgatcgtca ccgacgccgg cgacggcacc cgggcggccg agctccacgg ccaggagccc
     1621 ggcggcggct ggcggctgca cgcgcgcgcg cgggtcacgc ccctgtcccc cgatcgcccg
     1681 gccgagcctg tggcgctcga gcgcctgcgc gagcactgcg gcgcggcgct cgggggcgag
     1741 gagttctacg cgtcgatctg gcaccccgac ttcgagctgg gcgactcgtt ccgcctgatc
     1801 gagcaggtgc accgcgggga gacggaggcg ctcgccctgc tgcgcgtgcc cgacggggcg
     1861 acgggcgtgc gccccgagct gctggtgctc gacgcctgcg tccagaccct cgtcgccccg
     1921 gcgggcgccc acgtcgacca ccagctgccg gttgcgctgg gcaccggcca cggccgcatc
     1981 gaggtccacg ggccgctcgg cggcgagcgc ctctggtgct gggcccgcct ggacggtgcg
     2041 agcggcgccg cgggcgagat cgccggcgag gtgcggattc tcgacgaggc ggggacgccg
     2101 ctggccgagg tggacggcct gcgcttccgc cgtgtcgggc gggagaccct caagcggctg
     2161 gccgccgatc cggctgcgga cgcgccggac gctcggagca cccgccaccc cgcggcgccc
     2221 gaccgcgccc agctcgccgc ggccgacggg ccggcccgcc gcgaggcgct cggccgctac
     2281 ctcgccacgg ttctcggcgc cgtcgtcggg atgtccgccg aggagctgga cccggcgcag
     2341 ccgctgaccg ttctcgccga ctcgctgatg atcgccgagc tgaagctgcg cgtggagacc
     2401 gacttcgacg tcgaggtgcc gatcgagcgc ttcttcgacg acgccgggct cgacgacctc
     2461 gcggcctggc tcgccgacga ggccctcgcg gagccagccc ccgagcccgc ccgcacgaac
     2521 ggccaccggc gcgcccccgc cgcgatgacc gtcgagcagc tcaccgccgc ggcggagctc
     2581 gacccggcca tcaggccggc cgctcccgcc gatccacgct ccgccgccga cccccgcgcc
     2641 gtcctcctca ccggcgcgac cggcttcgtg ggcgccttcc tcctcgccga gctgctcgag
     2701 cgcacgaccg ccgacgtgct ctgcctcgtg cgcgccgaca ccccggccga gggggaggag
     2761 cgcatccgcg cgaacctcct ccgccactgc ctcgacgacc acgccgccgg ccgcatcgtc
     2821 cccgtcctgg gcgacctgac ccagccgcgc ctgggcctga gcgactccgc cttcaagggc
     2881 ctcgccgcca gcgccgacgc cgtctaccac tgcggcgccg tcgtcaagtg gacctacccg
     2941 tactccgcgc tcgcgccccc caacgtcgac ggcacgcgcg agatgctgcg cctggcgacc
     3001 gcggccagga cgctcccgtt ccacttcgtc tccaccgtcg gcgtcttctc ctcgctggag
     3061 tacgaccgcg cgagcgtcgc cgaggacgag ccgctcgagc gcagcggccc cctgaccgtc
     3121 ggctacgccc agaccaagtg ggtggccgag cggatggcga ccgccgcgcg cgagcgcggc
     3181 gtgcccgtct cgatccaccg cccgaacgtc ggtgcgcaca gcgccaccgg cgcgttcaac
     3241 ccgcaggacc acatctgcct ggcgctgaag ggctgcatcg agctgggcgc cgcgcccgag
     3301 tcgagcgtgc cggtgcagat ggccccggtg gacttcgtcg ccgccgcgct cgtcgaccgc
     3361 gcgctcgcgt gcgaccaccg caccttccac ctggtcaacc ccgagccgat tcgctggacg
     3421 gcgctcttcc agctggtccg ggagctcggc tacccgctcg cgaccatgtc gttcgaggac
     3481 tgggccgagc gcgtcggccg caccgccggc tccctcggcg cgctcgcctc gggcccgctc
     3541 cagcaggcgg ccctgccgcg cttcgactgt gcgcagaccc tcgccgcact gcctccggag
     3601 cgccgccgct gcccgcgcat cgacctcgac ctgcttcgca cctacttcgc ccgcttcgcc
     3661 gaaaccgggt tcgtcgagac accaccacga ggaggactga ttgaagggga tcggcacaaa
     3721 tcgccgcatc cggccgtcgc cgcggccggg acggaccagg gagcgcgggg ccacccaggt
     3781 ccggtcgcgg gcacggctcc ggcaaacgac agggaacgag gagaggaaca tggctacagc
     3841 tagccgagag gaaaggctgg aggagcggat ccgcgacctc gaggacgagc tccaccggcg
     3901 gtcgagcggc agagagggcc gcggccgcag gcagagcagc ggtcgctggg gcgagtacga
     3961 ggacacccgc gaggacttcg cggacttcac cgatcgcaag gtcgacgaga tctcgcggct
     4021 ggtcaccggc tcgttccggg ccgggctgga ggcgctgcgg gtggccgccg agtcgacgga
     4081 ctacttcgtc gacgacgtgc tcgagcgcaa cctgcccaac gcggacgagg actcgaccga
     4141 cgtggcgcgg cggctgccgc gcgacctcgg ccggggcttc tcgcggtcgc tggagcgcgg
     4201 gctcgacatc ccgggccgcg cgtccgagcg gttcaaccgc acgtacacgt acgaggaggg
     4261 cgggcagccc tggcactcgc ggcgcgggcg gcggggcggg cgcggcaggc gtggccgggg
     4321 tcgtcgggac gagtacacaa ggcggcgcgc gccgtccggc gaggactacg agcgctggtc
     4381 gcagcgtgag ctctacgacg aggcggccga gcgcggcatc gactggtcgg acctcacccg
     4441 tgaggagctg gtcgaggagc tccgcaagga tgagcgcggg tacgaggact ggccgacgcc
     4501 ggagctctac cgccgggccc gcgatctcga gatccgggac cgcgccgaca tggaccgcga
     4561 cgagctgatc gacgcgatcc ggcggcggga ggagcgcgcc aacccggccc ggtcgccgct
     4621 cgagcggcgg cgcggtggct ccggcgacgg agagtccacg gaggaggatt acgacaagtg
     4681 gtcgcggagc gagctcgagg agcgggcggc cgcatgcgga atcgagaacg cgtcggagaa
     4741 gagccgcgag gagctggtcg aggcggtcaa gcggcacgag ccgccgccgg aggaccagtc
     4801 ccggacggag ctctaccagc gggctcgcga gctcgacatc gagggccgct cccagatgtc
     4861 gcgcgaggag ctcgtcgagg cgatcaagag ccacgagaag tcatgaacta cggcgatggc
     4921 ggatcggggc ccggcggggt cgcgagcgcc acgccgtggg tcgtgcggtt cgacccccgg
     4981 cccacggctg cggtcaggct gatctgcctg ccgcacgccg gggccggggc gtcgatgtac
     5041 ctccgctgga gccgggcgct gcccgactgg gtcgaggtgg tcgcggttca gctgagcggg
     5101 agggagacgc ggcgccggga gccgttgcga catgccgccg gggagctggt cgaggacctg
     5161 gccg
//
